LOCUS       DAOONL010000001       114678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_53_length_114678_cov_20.710355, whole genome shotgun sequence.
ACCESSION   DAOONL010000001 DAOONL010000000
VERSION     DAOONL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 114678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 114678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_114678_cov_20.710355"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..971
                     /locus_tag="PLP50_00005"
     CDS             138..971
                     /locus_tag="PLP50_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA49952.1"
                     /translation="MLRRPLVSRLGCGAAILAAVALPAVMLTPLVRSMIFPASQAPFP
                     EPRPQDGWSVARYETADGVRLAGAFFPAPSPDAPVLLYFHGNAEAAAHNLPLADDLRA
                     RGFGVFLMEYRGYGGLAGSPSEEGLYADGEAALAELGRRGISPERVVLVGRSLGSGVA
                     VELATRHRVAAVVLVSAYTSLVDMGWIVAGPLAPLVVRDRFDSLAKIGRLEAPLVLLH
                     GALDDVVPVAMGRRLAAARPDARWVEVPGTSHNDFPGLAALVAREVDAALADARNAPP
                     P"
     gene            1024..1587
                     /locus_tag="PLP50_00010"
     CDS             1024..1587
                     /locus_tag="PLP50_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49953.1"
                     /translation="MGTVEPPPGQPAPYAAPAVAASPGRSPWTIALLVLGALAAFLWF
                     RRPSYLPDMPLPSASAEGVSAGPDASPVFCRGSKGCLVVYVAPWCGPCRSSLPGDVAL
                     ADHIAPMGYETVFVVGMDDVRACLEMTKSIGRSARIDSERKWARAARISGVPHFLVTT
                     PTGKIMKRQAGAYLAPPDQVAQLLGIS"
     gene            1623..1919
                     /locus_tag="PLP50_00015"
     CDS             1623..1919
                     /locus_tag="PLP50_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49954.1"
                     /translation="MSALSTRLDDPGAIPYILWDDPMTVSELEERLRTASEPERLRLV
                     ALILREARDADVWRFVTPEEVIRLWPRLAGRLGLRFAFWEFLLDRWKRLGLVEA"
     gene            1923..2573
                     /locus_tag="PLP50_00020"
     CDS             1923..2573
                     /locus_tag="PLP50_00020"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPA49955.1"
                     /translation="MSRLSRLQEDVLHGFFRRTRSFFLTGGAALAAFHLGHRATDALD
                     LFTSSADLDEGVSALRSVAEELGASIQALRTSPAFRRFLIGVGAESVIVDLVRDRAPQ
                     IHVEKLDRDGVLVDPPAEILANKLCTLLSRGEVRDLVDVLFLERAGFRAEDAMGPASS
                     KDGGLTPAQLAWVLSQITVGPDARIPAGITPDELRAFLENRVSRLTRLAWPGAPAS"
     gene            complement(2574..4679)
                     /locus_tag="PLP50_00025"
     CDS             complement(2574..4679)
                     /locus_tag="PLP50_00025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPA49956.1"
                     /translation="MPPEAPPPAPKRYVLRKAGPAKSYRIDYRRELNAEQQAVVFAPD
                     GPTVVVAGAGSGKTRTLVYRVSRLVEDGVAPEAILLLTFTNRAAREMTRRVERLLGAD
                     LARMTAGTFHSVGARLLRPHAELLGYRPNFSILDSEDSKDLLESATSDLGIPITERRF
                     PKGDLLRAIVSFAANTGQKLDAVIGRDYPHLLSQLEPVKAVIRRYLERKVLANAMDYD
                     DLLLNWRRLLETHPEVRKALAFRFRAILVDEFQDVNRLQSEIVDLVAQDSEAKNVMVV
                     GDDAQSIYSFRGADAEALLGFPDRYPGATVYRLETNYRSTPEILRLADASIAHNSRRY
                     EKTLVPTRESGVPVAVVGAKDLLQQAEFVAQRVLELRDEGTPLSEIGVLYRAHFQALE
                     LQIELTRRGIPYEVRSGLRFFEQAHVKDVLAHLKLLVNPKDEMSFKRALKLLPKVGER
                     TAAVLWEAVSDSHEPLAAFLALELGKAPAGARAGLLRFRETMRTLKKPSYVSSPAEAI
                     RYVVEDGGYGDVAKGKFPNHSARLDDLEALAQFALAYDDVGRFLEEVTLYGDPSGEDQ
                     VAGEEDDERLVLSSVHQAKGLEWKAVFVIGLQEDRFPNLRACKTPEGLEEERRLFYVA
                     ATRAKDELTLVHPLTAFDRYGVVIVTEPSQFLRELDATLYERWVLDESVDASLSPAPE
                     RPRLAADPTLVPDDEEPVN"
     gene            4766..5452
                     /locus_tag="PLP50_00030"
     CDS             4766..5452
                     /locus_tag="PLP50_00030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HPA49957.1"
                     /translation="MSKPIPEGLWELLPLLFRPHPWHGVSVGDEAPDKVTAYIEIVPT
                     DPVKYELDKTTGYLKIDRPQKFSNVYPSLYGFIPQTFCAEENAAFCALKTKREGIVGD
                     GDPLDICVLSEKDISHGDVLMDVIPIGGLRMIDNLEADDKIVAVLEGDAVFGQLREIY
                     QLPPALVDRLKHYFLTYKQAPDSLQPHKVEITHVYGREEAHEVIRRSRADYDKRYGKL
                     RELLTAALRA"
     gene            6278..7078
                     /locus_tag="PLP50_00035"
     CDS             6278..7078
                     /locus_tag="PLP50_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49958.1"
                     /translation="MGDAFDDFLAGKPAGSGRLRVPQASVSPQSAETVMAPAEASEAT
                     RLGPAGQVGVPLPAPTPDPPASTRGRRPRRFGVLGLAGAVVGLVVLLGVVTLLVGPGR
                     DREPDPSRTTPSPRSTPSAPAAAAPTPAVADRSVAEPPEAHATPTAAPNAESVPAAPS
                     RRRAPAEPLPEPPPIRRPVTTPERLDPEPPFAGPPPAACSGARVRIGFAVDETGRITA
                     PRLFPSEAPPECGRFVLDALPAWKWRPAVDASGVPVASARLIVEIQLP"
     gene            7128..7805
                     /locus_tag="PLP50_00040"
     CDS             7128..7805
                     /locus_tag="PLP50_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPA49959.1"
                     /translation="MTRSRPLLLLVVASTALLPACTPKNALRMAIEVVSEPDAAIVSF
                     KGKDVGTTPTALRVRTFDELMTIAARKDDAPVAEKRIRILSPDHAQLVFRFGTEPSEV
                     AKRLGLTRVVVFDYSEKVSFDVSKSELKPEGLPILDRQAEILARYFPNVDVYVCGHTD
                     ATGGDDFNLQLSVDRARVVADRLSGQGVPPERLKVQGFGKEFPNDTNETPAGRANNRR
                     TELILPQ"
     gene            7886..8152
                     /locus_tag="PLP50_00045"
     CDS             7886..8152
                     /locus_tag="PLP50_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPA49960.1"
                     /translation="MSIDAIRLFGEAVRKDPALLEKVKAVGSDVDGMVALARESGFSF
                     TAEELREAAGAGKGELSEEQLQKLSGGGSWVQVQTVAVHVAVVG"
     gene            8244..8513
                     /locus_tag="PLP50_00050"
     CDS             8244..8513
                     /locus_tag="PLP50_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HPA49961.1"
                     /translation="MSKEAIAKFQEAVRTQPALLEKVKAVGSDVDALVALAGEAGFVF
                     TRDELMDAAQAKKGELSEEELQKVAGGTAVGVLTVAVAVVAVAAT"
     gene            8527..10026
                     /locus_tag="PLP50_00055"
     CDS             8527..10026
                     /locus_tag="PLP50_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family RiPP maturation amino acid
                     epimerase"
                     /protein_id="HPA49962.1"
                     /translation="MPGRGAAGPEPPGLPACSGEPVAASTGPSTDGSPFDFHRRHVGR
                     FSPSERSELAHTKRFLECVLGDGEFRAALGAGEEARLQACRQRGLQAKPADLEPLWRH
                     GTVFEVPTEEAGRYPAVALWIEWLGGLLRFRDLLREHGRTTACHPAFDAWRLRQIARG
                     RRELGHASAAITHPVIAFELSKGCTFGCWFCGISAASYEGHLPYEGAGRALWRDVLAS
                     ATDFLGPAMQTGTCYWATDPSDNPDYTRFLDDFREVTGALPQTTTAGAIRDVRWTREV
                     LAQAATGPGTPTRFSVLSNGMLRRIHETFTAEELLTVELVLHNPESALGVARSGRALG
                     REARRMSDTQGTIACLSGYLVNMVDRSVALLSPCNASERWPLGYRVHARGRFASAEEF
                     RAFLEETAAECMPLHLRGEDPLAFRPGIEHAATADGFALSSRFTRQEFSGGATARRLG
                     PLVAAGGSTVGEVIGTLAAEGLDVLAATTLLQSLYDCGVLDDELSGSWG"
     gene            10030..12003
                     /locus_tag="PLP50_00060"
     CDS             10030..12003
                     /locus_tag="PLP50_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HPA49963.1"
                     /translation="MDVLFVNMPWIGLARPSIALGTLKSVLSAGGVRSEVVYANLLFA
                     SEIGVEAHQAVSLVYANHLLGEWLFSSVAFPGASSGSDAYLDRVLSRDIFHKAYWKPE
                     RVRELVLAARARAEPFVSRLAEDVVARRPRIVACSSTFQQHVASLALLRRVKELDPGI
                     VTLLGGANCETVMGRTTHRLFDQVDFVVSGEADDVLVPLVRAIERDGREVSPEELPVG
                     VLGPAHRRSGYPRVADAQGADDTPRAFARDLEAIPPPDYTEYFETFRSLDALVPAVTP
                     TLLVETSRGCWWGEKRTCSFCGLNGRENAYRKRSPGRVLTELDDLSRRYSLATFETVD
                     NILPMSAFEDLLPALVEAGSPYQLFFETKANLTRDQVRLLAEAGVRWTQPGIESLSTE
                     LLRLLGKGCEAWQNIQLLKWSRQFGIRLSWNLLSEVPGDRDEWYGEMAEFLPLLEHLQ
                     PPSGVAPIQYHRFSSYHRDPARFGLRLRPAPGYSAVYPLPEEELADLVYLFEDGWRDT
                     VRKSPVLKMLLDGPGLPRLRQRFLAWAGRWVEGPAPVLAATYTESAIHVSDTRSVAPK
                     AERTVTGLDRLVLLEADRAPLEKGLVARLVADGHEAKFVAESVERLLSQRLALRVDGR
                     LVGLVLQDPVAPLPALEEGPFGRELPRERPAET"
     gene            12000..13091
                     /locus_tag="PLP50_00065"
     CDS             12000..13091
                     /locus_tag="PLP50_00065"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPA49964.1"
                     /translation="MIVTPHAATLPGPFRLDGGGVLEDVEVAYEEYGEPCPGRETVLV
                     CHGLTTDAHAAGRHTWIDRSAGWWDAAIGPSKAVDTDRYRVLCANVLGGAGGTTGPGS
                     IDPRTGRPFGLRFPVVTVGDMVAVQAALADHLGIERFHAVVGGCLGGFQVLEWMARFP
                     ERLERAVVISATPRTSAHNLAFFEVLRQAITRDPRFVGGDYYGGEVPADGLALAAMFG
                     AVVWLGRETMERKLGVTLLEGTKPRFTLGPEFTAQAFLAQIAENAPARLDPNSFIYLT
                     RATDYFDLARDAGSLAAALAGFRGRSLLVSYASDWRYPPSGVDEIRVALEANGAPVRH
                     ETLESEFGHGAFVYDVEGVGDLLRRFLAD"
     gene            complement(13105..14736)
                     /locus_tag="PLP50_00070"
     CDS             complement(13105..14736)
                     /locus_tag="PLP50_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49965.1"
                     /translation="MTGPGDPLLAGLYASVWRTVCWHLDGAAIGTSVEAMESRGLFEA
                     LREASAPLAADELASRAGARPGLLDVALRLLADQGFVSREGGGEPGRSRVRLTATGLD
                     RLRTLGTYSGAAARIDRARALLRAFAGDGAARPLVGADDLAIEPGGELIERRARAHRH
                     GPLVAAAVTGLDRCGALARLAEAGARGCPDEELPGRGRAVSAALEVLAARGWLKREAG
                     RTSLTFEGRVVSEVAPQYWYAASYLPTLGRAGRRIFTAPAARESGVDDHDGVERHVDR
                     ALDIAFSGVVFRRACLEPLLSAVLPLFESEPLESQPAAIVDTGSGDGTLLVELYRAIR
                     ERTARGRRLDAVPIRMVGVEYNEVARVETERRLRAEGVPGGSARGDIADPDAIAATLR
                     ERGLDPHDVLHVSKSVIHNRRFVPPGLDDGLPTFGDPDGVFVDAAGERIAVRRLELDL
                     VQLFRRYRPFTARHGMVVVEAHAVPPEAIAAAIGRAPLTLVDACHALSGQLLVEAPVF
                     RAAARAAGLASAFSRDLDTAAVGRPLLTIDHFRPV"
     gene            complement(14780..17692)
                     /locus_tag="PLP50_00075"
     CDS             complement(14780..17692)
                     /locus_tag="PLP50_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HPA49966.1"
                     /translation="MPDAIDPRAALDAIATAAGGAGPSRRGGPLSAFSIAGGTRAWLV
                     REGSVDVFFEPAEGRGPRRHVCRVEAGDALLALPALEGEGGVRAVPGPETALFEVELA
                     RLLPLAGDAAIGGAVVSLVEGWLQALAAALSEDRLPPRTSRTPAEEAAVAGEGEWLVA
                     QRGVRWVGVRAGELLPYGEPGVAPFGAGAAAPLCAGFWLKVARDAELEWLGTAEVAGR
                     PGGLPNALASFGRSVVELLERRQARAAEEARAQLLRRARSVRALRSEAEERLASVLGT
                     SLPIASAGDDALAVACRLVAEAGGVAIRPAPAGAPEPRSLAAIAADSGVRTREVLLRD
                     GWWTRDGGPVLTFAKDDRRPVAILPDGPGRHVLVDPVRGARTRVDAAVAATLEPHAHV
                     LYRALPPGTPGRRALLRFLGFGSGPDLGRLFGLGAVAGALALLTPIGTGLLFGSVIPS
                     DSRSQLAYLVAALVAAGFAVAGLQVVQSFAQLRISGRAGGAVQAGLVDRLLALPPSFF
                     RRFATGDLANRALAVGFVQERLTGAASMSVLSALFGLFNVGLLFWYSPRLTWFALLPV
                     ALAVVVAAVLFRLLMHHQVALYEVAGRIQGLTLQLTEGIAKLRVAGAEERAFNRWARA
                     FAEQKRLDLGVRKVLAGVVVLGAGLPPLATGFVFALVGLSTDRSLTSLSLGTFVAFLA
                     ALGAVVSSLTMTLSLLFPVLDVVPVWKRSRPILETPPEPSSRRADPGELSGAVEVSRV
                     SFRYHADGPLVLEDVSFRAEPGEFVALVGPSGSGKSTLYRMLLGFESPETGAVLYDGL
                     DLAGLDLAAVRRQLGAVLQNSQLLNGTVYDNIAGTSRMTMDEAMEAARLCGIEDDVKA
                     MPMGLHTMVGVGGAGLSGGQRQRILIARAIARRPRILLFDEATSALDNHTQAVVSRTL
                     EQLRATRVVIAHRLSTIRNADRIVVLVDGRVAEAGTFDELMASGREFASLARRQIA"
     gene            complement(17685..19877)
                     /locus_tag="PLP50_00080"
     CDS             complement(17685..19877)
                     /locus_tag="PLP50_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HPA49967.1"
                     /translation="MPAEDVTRRARPARRRTPTVIQMEAAECGAASLGMVLAYHGRFE
                     PLEKLRLACGVSRDGSNAGNVVRAARHFGLEARGFAMGPAELRDLAAPFVVFWEFNHF
                     LVVEGHGRKGWWVNDPASGPRLVPDADFDTSYTGVVLTFRPGPDFQPGGTGPSLFRSL
                     ARRARGLGGPLAVAAATGFALAVLGLVLPSLTRVFVDEILVGGHRSWTTPLLWIMGTA
                     IAMLLALTALQQRTLYRLQAKLSLTSSSRFLSHLLRLPMDYFGQRFGGEVGARVAIND
                     RVAQLLSGQLATNVLGALMAVLYLLVLAQFDVVLTAVVAVLAATSLLVLAAVSRRRRD
                     LAKRVLQEQGKLLGVTLNGLQIIETLKANGSEPAFFSRWSGQHARASNSGQELAAATQ
                     VLNAVPALLTTLNTAVVLGVGGLRVMQQQLTLGELVAFQALVAAFLLPIGTLVALGSQ
                     LQQVDGDLTRLDDVLENPVDLQWYGPEVPALDAASAADPPKLSGHLELRDVTFGYSPL
                     AEPLIKDLSLTVRPGGRVALVGVSGSGKSTVAKLVAGLYRPWSGEILFDGKPRTARPR
                     EVLAASVASVDQEIFLFEGTFRENLTLWDGTVPDSDVVDAARDACVHDVVTARAGGYG
                     GAVEEGGRNLSGGQRQRLEIARALHANPGLLVLDEATSALDPLTEKRIDDALRRRGCS
                     CLIIAHRLSTIRDCDEIVVLERGRVVERGTHDELVLSGGAYSRLIQDA"
     gene            complement(19896..21143)
                     /locus_tag="PLP50_00085"
     CDS             complement(19896..21143)
                     /locus_tag="PLP50_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HPA49968.1"
                     /translation="MSGLFRREALEKLQSPDRLDELLVVTGPRSWIAVAALSTLLLAA
                     TIWGIWGRVTTSVDGMGIFIRGGGLVRVVSVGSGTLWQLRAAPGAVVREGEVVAVLSR
                     PDLTARLRNMQSQLAHLEEQERRAVAFAQEEASRQRDVLRRRIREAEQQAGAARTRLE
                     WLETRIVGARKLVDAGLTTRQPLAELEHTRDATRIELEQAVTAAGALLAQIGDLDEQE
                     EMKVFRIRQELDDERNEVAQLELEHRTNTEVVSPYAGRVTESLVNPGSLVSPGQAILA
                     LERDAGTLRVGVAVPALEARSIRPGMEVRLTPTTVKLEEYGYMLARVTDVSDYPASRD
                     GLTSLLSNEALVEVLTAAGPVTIVSAEPVLDLAAKNGFRWSARKGRTLSVSSGTLCTA
                     QVMVRETAPITLVVPKLKELLGL"
     gene            complement(21324..22028)
                     /locus_tag="PLP50_00090"
     CDS             complement(21324..22028)
                     /locus_tag="PLP50_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49969.1"
                     /translation="MSLSRILSRAALGALTAGAVITLFAPLFPFERTAQAGDGTEETT
                     ITIATSKDKEATVLKLDPMKPGETKTLTSESGKPVVVTRTEDGYTLKVGEKEFTVKTL
                     ADGEGPHVLLPGGKEVHVVRVGEGDGPAMVFTGDDGKRVVVKKRAYAYTLGEGTERPS
                     AADVLEKAAPKSLESLDVRTRGAVEQVLQELIESGAVFAPGAMPMTWAAKEGEDGDAV
                     KVMVIRKKQEDRAPEK"
     gene            complement(22180..23547)
                     /locus_tag="PLP50_00095"
     CDS             complement(22180..23547)
                     /locus_tag="PLP50_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA49970.1"
                     /translation="MPEARVLLVEDEIDQRKIVAGILRGEGYSVAETGSVDAALAELA
                     ASPVDLVLSDWKLPGRDGTELLAEVKARFPETGFLMATAYGTIAHAVTAVRAGADDYL
                     TKPFERAALLLAVERTLRARRLADENRRLSEEVRDRDRLVDLVGKSPSMERLYRQVEK
                     LAGTDATVLLTGESGTGKELAARALHALSRRRAGPFVAVNAAAIPDTLVESEFFGAEK
                     GSYTGADRTRKGRLELAQGGTLFLDEVGELPLPMQPKLLRALQEGVVTRIGGADEIRT
                     DARIVAATNRDLALEVSSGRFREDLYYRLNVVSVTLPPLRERREDVPLLVRHFVARTE
                     RRYGIRVEPFPSALLTRLVDHSWPGNVRELGNVVERLVLLSEGGRVSAGDLPEPLGGR
                     PAADAGWKLPPGGLAWEEHEKDALRQALELAGGNRARAARLLDLPYKAFLYRLEKFGL
                     APADE"
     gene            complement(23540..24952)
                     /locus_tag="PLP50_00100"
     CDS             complement(23540..24952)
                     /locus_tag="PLP50_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA49971.1"
                     /translation="MTLRVKLFALMGGLLALMVGAEWLLVEALTRDLRVEVTAVATSV
                     GKDVVKVLQRRDGKLLELGGDAGVRRIVVRDEAHLVTLPPRPAADAPAKDRAAGPEAV
                     PKEGEGPRFVVTTVQTDGNRTTSSTKTWATRGGTGFIFLEGPVEKVKIPIPGAGVKDA
                     VGRFRTRLVLGSLGIVAVGLLAAAFVAHRVTRPLAALARAARTVGEGEFGARVETREG
                     GEVGAAIASFNLMSSRLQELEREALALKEREHLGEMGEVARGMAHALRNPLHAVGLSV
                     DELAARLPDDPDAAAMAAAARRQIAGVDGTIRSFLALAAEGGAEEEVDVRALAEEVAL
                     AALQEARGRVRVSVAPGGAARLTAVAPELKAALHALVVNAVEASPDGGEVVVSVAGEE
                     GRAVVRVEDEGSGLPGEVRARLFTPHVTTKPSGSGMGLFLAHRIATTRYGGSLALEAR
                     APRGTRAVLAVGHRGGGARA"
     gene            complement(24949..25701)
                     /locus_tag="PLP50_00105"
     CDS             complement(24949..25701)
                     /locus_tag="PLP50_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49972.1"
                     /translation="MPRYASWTAKALLLAATSALAAPPADPLDAKYGAAVPPAYVKQY
                     EADRSGRVLLNPYLQVATRDFPALLEPGDGTYKWVIDVAGRVAILREVPHPLGRTYPN
                     GFHRPEDGSDREPGYVETYGHVSALGGAPGRIGGEILWDADSRAFTVNNKSGRYTKHE
                     ADRTPEQLVEAARLIREVVDPGGASWGEVFYLLDYAPPDVRRKLLDDPRLEYDLPETK
                     GRPYVVVLGGGTSPLAGREGGDGTDLVSSSPR"
     gene            26037..26534
                     /locus_tag="PLP50_00110"
     CDS             26037..26534
                     /locus_tag="PLP50_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPA49973.1"
                     /translation="MAQEIERKFLVAGPFKEQATRQTRIVQGYLSSVPERTVRVRIKG
                     DKGFLTVKGIGNASGASRYEWEREVPVAEVEQLLAICEPGVIDKVRWLVPAGEHTFEV
                     DEFHGENEGLVVAEVELGAEDEAYARPAWLGTEVTGDPRYFNSMLMKNPFTRWERPAD
                     GAEGA"
     gene            26531..28135
                     /locus_tag="PLP50_00115"
     CDS             26531..28135
                     /locus_tag="PLP50_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006986974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPA49974.1"
                     /translation="MSGPDRPAAAADGTPRAPTAVDPLDMRNYGLEKLQEIKPGPFMA
                     RAKLAGFPLGILAFLFFHFRWCGPIGFFEAATGVANVGHLYTVTGIFFFSLVLWMTEA
                     IPSYLTSFIVIVSTVLFGILKMRPTFAYLGEPVMILNIASFILASALVASGLAKRIAL
                     LLVTRWGNHITGIFWAFIALNLLLGAFISATSAKTAILLPLFMVIAAMYGAVGGVSRN
                     NVGRNLVLQNLLANNVSASAFITGSAANLLAAQLLENAGHKVMYSEWLMALLPLAVIQ
                     CAIAWFTGTKVIFPVRHEDAQPKVDGGMDRLRAELAALGRPSLRELRAAVVFVAVLAL
                     WSTEPLHGIRAEVVAMCGAVLVLLPPFSGLPRFGVLTWNEADIPWHMLMFSWGAYVLG
                     GIMESTHIVDLAVEAMFGHLGITAATPKVAVFLVLASLFAFTTLINESKTARTIVLFP
                     VMIATATKFGWDVVGFCLPMAFLINQVYVLYFNSKPANISYLSNMYSTGESFKFGITQ
                     LVLILVVLTLWTQYVMPLMGFQSRLW"
     gene            complement(28208..29254)
                     /locus_tag="PLP50_00120"
     CDS             complement(28208..29254)
                     /locus_tag="PLP50_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012140721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPA49975.1"
                     /translation="MSWLFLSSGLFLGWSLGANDAANVFGTAVATKMVRFRTAALVAG
                     LFVVLGSVAGVSGAAGTLGRLGAIDAPAGAFTVALAAGIAVLLLLKRSGLPVSTTQAI
                     VGAIVGWNLYTGRATDPAVLGRIVSSWLLCPLLSGALAALLFVAARAAMPHVRIHLLE
                     VDAWTRAGLLAVGAFGAYSLGQNNIANVMGVFLRVSPFPEEVALGPLRASSVEILFLA
                     GGLAIATGIATYSHRVMKRLGAGLTALSPLAALIVVAATATVLWVFASPSLSQLLRAH
                     GLPAPPLVPVSSSQAVVGAILGISLVRRSPVKWRTVGGIVAGWVLAPAVAALLSIVGL
                     FVLANVFLVPVVAP"
     gene            complement(29277..30368)
                     /locus_tag="PLP50_00125"
     CDS             complement(29277..30368)
                     /locus_tag="PLP50_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPA49976.1"
                     /translation="MSWLFLTSGLFLGWSLGANDAANLFGTAVATRMLKFRTAALVGG
                     VFVVIGAWAGAHGTSKTLGRLGAIDAPAGAFVVALAAAAAVVLLLTRSGLPVSITQAI
                     VGAILGWNLFNGKATDLRTLTQLLSSWLLSPVLAGLIAAGLFVVSRRVLQRRRVHLLE
                     VDAWTRTALLVAGAFGAFSLGLNDIANVMGVFVRVSPFPAETAIGPFRFTSVELLFLL
                     GGIAIAVGIATYSGRVMVRLGSGLTSLSPLAGLIVVLAAALVLFLFGSPALQRLLRAA
                     GLPGWPLVPVSATQAVVGAIVGISLVRKSGIRLKPLGQIAIGWVAAPAAALLFALVGL
                     FVVQNVFELPVSAMPRPAAGASAVAGSAS"
     gene            complement(30385..31029)
                     /locus_tag="PLP50_00130"
     CDS             complement(30385..31029)
                     /locus_tag="PLP50_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPA49977.1"
                     /translation="MTLLFRRTRALEAQIDQFLDAVSEGCLVFEKGVDDYLRHDEPSF
                     EERRKAIGVFEGKADALRREVETRLYAESLIPDHRGDVLGLLENLDNVIDQAKQTLGQ
                     FEVERPEIPEELAPDYLALARACVQATQHLVDSCRAFFREITAVRSHIHKVYFFEKEA
                     DRLADGLKRKIFAMDLELARKIHLRYFCLHVETLSDRAEGVADRLVIYTIKRTI"
     gene            complement(31026..31256)
                     /locus_tag="PLP50_00135"
     CDS             complement(31026..31256)
                     /locus_tag="PLP50_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49978.1"
                     /translation="MKWTPESGRPPLLLVVVALLLLLAAVLTGPALGSDREKGDEPDV
                     EGDRRGPASRPAQTVLAGAKPLLVSVPAGGAA"
     gene            complement(31498..32100)
                     /locus_tag="PLP50_00140"
     CDS             complement(31498..32100)
                     /locus_tag="PLP50_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49979.1"
                     /translation="MSNAAPVIIPRWEYRTFGESFGEAESNIRSHGEAKARSSAETYI
                     VSAESPNNTKIRDGLMDIKKLENVNGDTLEQWNPVLKSGFPLTKEVLSQVAAAWGVAL
                     PELARAGYSLDEFLAEVVGAHGKLRAIAVGKERFGLTVDGCIVELANLTFDGTPIRTA
                     AVEHEDPALVIATVRKLGLDKFPNTNYIKALKRFAGLPVG"
     gene            complement(32208..33563)
                     /locus_tag="PLP50_00145"
     CDS             complement(32208..33563)
                     /locus_tag="PLP50_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPA49980.1"
                     /translation="MKRQFGIPVLVALLAAPGAALAQSDAERIADLEKRVESLVKKDK
                     ERSAAEAKANSAKGTTLRSVSDKGKLEWASADGAYKFRLAGRAQLDGVFFSGDENRNG
                     NALGLRRGRIGFKATVAKDWTAEFDVDFADNAVDIKDAYVGYEGIDKSAIRLGQFKIP
                     YGYDQLVSSKDIWFVERAYVDAFAPGRRLGVGYFYGADRFSAAATLFTQGIGEDVSGL
                     DQGWGWAARGTWAPVMPAETRAIHVGVAATGWQPDAAVGDGSTRPPAAYVVEFSSRPE
                     VSKIAKAKYLNTGDLEDVDQVRQYGLELAGVWDAFAWQAEYNKTSVDRLSGAVDLSDH
                     DFDGWYAQVSYVLGGKRKYAADEGLVDKVSPGKGGAWEIAARYSTMDLNDLTAVDPIK
                     GGSAENYTLGLNYYPNFNIRLMLDWSIVDNDEYAKPKKLYGAIAGDDFSVLQFRVQYA
                     F"
     gene            complement(33600..34364)
                     /locus_tag="PLP50_00150"
     CDS             complement(33600..34364)
                     /locus_tag="PLP50_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPA49981.1"
                     /translation="MKKTPFAALSLALVLVAAALAPAVAAQGVTPHVSVLRGESNFLT
                     DFDPINLDGTINAVIEIPAGTTAKYETTKDTGMLELEQKNGKPRFVQYLGYPCNYGLI
                     PRSLLPKSKGGDGDPLDVCVLGPTVPIGSVVKARPIGILTLLDNGEIDDKVLLVMQSG
                     PFAKVSSLADLDKRFPGVTTILQTWFTHYKGYGKDGKLQLTSPGFKGRAVAIKTIGDA
                     ILDFETSVVTEADKRPLDEKGNPYTYRWPGARNIGE"
     gene            complement(34407..36152)
                     /locus_tag="PLP50_00155"
     CDS             complement(34407..36152)
                     /locus_tag="PLP50_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49982.1"
                     /translation="MHKRAVSGLLALALVIGCAGVAVAADEPGPLYVQTIQKYRAPVL
                     DDVGGREYKFLIDPAKMKAKPAEAFKDIWKRVRAAAEKNGFEVTEKEKNPFKVELATK
                     EYLDTPDQLLWKAGYQIRVTNKYADGKPARDVAVNVKCVKEDARLAMAAPLAVTGGEK
                     VKIEAEGNVGIGPGGSLREYIEKGATFTVPVEALGKMTLADFGRFVPELLSIGLPADT
                     ALVGKKAYSSRVKPGAVVLPGTEPCGVSMEAFSDKEGGAPYLYDFSYGYSDLYFYDIA
                     KVHEAGERFLTKVLHGELAAIAMPESEKWGGSKVRKLMNRPLGAAAVAAGPLVDPLDE
                     LYGVASQPAYIKHYEADRDGKVLISPYLQVATKEFPAILDAKMAPYNYVIDAEGRVTI
                     IPEAAHPLGRVYPNGFFRPEDQSEKKPGTRENYGHVSSLAGGPGRISGEILYDKETGT
                     YTVNNKSGRYSKHNKDRTPQQLANAARLIRQVVDTAGVAWGPALYLLEYAPADVREQL
                     MKSPDLRYDDEKKKKRPHVAVLAGAPSTLSTDAPIAVAAAPVAAAAAVEAPKAAAPAA
                     PEKKKAKAAHNDDPS"
     gene            complement(36365..37714)
                     /locus_tag="PLP50_00160"
     CDS             complement(36365..37714)
                     /locus_tag="PLP50_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA49983.1"
                     /translation="MPEPKVLLIEDDPAVRHGMAAFLRANGLDVDEAETCQRAMERFR
                     LVGHDVVVADYSLPDGTSLDILPQVKKLSEETPFVILTAHGSIDLAVRAIKDGAEQFL
                     TKPVESKALLVLLKRLLQQQRLRKRQEIVARTQPGLLDPFVGTSPQVRRLDERARLML
                     EWDSPVLILGETGSGKGVLARWIHANGPRREEAFVNLNCAGLTRELLESELFGHERGA
                     FTGAVNPKPGLLEVGHRGIVFLDEIGDMDPAIQPKLLKALEEKTFRRLGDVRDRHVDI
                     RLVASTNLDLEAMVREKRFRDDLYYRISTLTLRIPPLRERPEDILVLAREILAGVCGR
                     MGRPSARLTEDAEAALRRYSWPGNIRELANVIERSMILVKGETLDAVGLGLDAVAAPP
                     PRDHDRLLTMKEMERLHVERVLATTGGNVTEAAAILGMARRTLYDRMKSLGLGGARE"
     gene            complement(37707..39590)
                     /locus_tag="PLP50_00165"
     CDS             complement(37707..39590)
                     /locus_tag="PLP50_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA49984.1"
                     /translation="MTETVGQRSGWRIFTMGLSSRTAQALARSWTSAPLVVDVGGWTE
                     SELEAVACGPDDTLVAGADACRTHAEKVRTFRAAHPRVPALAHLPRSRSGLLAEALRA
                     GFLEAFPGVAGFAAALERARARRDALAAPQAAAASGFPSEGGEAPLETAVPNAFDWIY
                     VVGVGEDGALGFETVNPPLNAGAGFLSPGFAGRPVRECLGEPAFGRFVPYLSRVLEQG
                     LPLPFEEEHVVDGKTRAFQTVLTPVRNKWGRIHRVAGISRDVTALKEALAELRASEER
                     LNHALEGTQQGLWDWDLSTGKVYRSPRWFGMIGLTPGGVEDTFDAGLRLIHSDDRRQI
                     EAAMSAHLEGRVPRFEAEYRLRTRTGEWIWVFDAGKVVAWDGDRPARVAGMCTDITER
                     RRAEEALRALVVGVVHEIRNPVYGILINLDALEATFGEDPRYVSFVAALRESAERIAS
                     LVNDLRDYGEPRTLNPEPHLVRSLVENAVRACDKLAADRSCDVAVDLEEPTLVLPLNP
                     RRMHQVFRNLLENALLHSPEGSTVRIAGRRTFADGFYWWTFDVEDSGAGFDAEALSRG
                     FEPFFTRRKGGTGLGLSIVKRVVEEHGGGVEAANRPEGGARVTVRLPAPHQPLQSAGG
                     ARA"
     gene            39811..41622
                     /locus_tag="PLP50_00170"
     CDS             39811..41622
                     /locus_tag="PLP50_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49985.1"
                     /translation="MTDRAAHPRGATSYLRAVLAALVVASAGCGGGETLPAPLPSGTP
                     RPLGGASALELFPALSPSGDELASSVMTNGKLEIHIRSLSGGPGRPLTRDGRNNVQPA
                     WSPDGRLIAFHSKDRGGIWIVPASGGSPRLLASFGSQPSFSPDGRRVAFQSTPLTDVA
                     ATAAPGLAPSTIWVVDAAGGAALAVTNPGSPIGGHGAPAWSPDGDRVYFVTSEPRLTQ
                     AEIWSVKPDGSDLVRLHAGTRIYGVCVPRGAKALWFAGQGKASLYALHRISLDERGRT
                     SVGPAEAMTPPGSAVPHSPSFSADGSRLAWSELTTTAAIWSLPLDPATSLAAGPPAPL
                     TGGDGRATWPVFSPDGSTVAFGWVPTGGTNDVWLMEPDGNHQRPVVEGPSIDYASDFF
                     PDGRRLLFLSNREGGTFAPWELEIATRTFRRIPAELHDAIALRLSPDGKRIAYHSKRD
                     SITINAWVVDLATRTSQQMTFDREFVGYPCWSPDGRELALEVRRGDDIFIGVIPSAGG
                     TPELLTQKRGLSWAFSYSPDGRRIAFAGYRDDTWNLWWIDRTTREERRLTENLRLDVY
                     IRYPAWSPKGDRILYEQAETRGEVFLLDGRAPSAAAR"
     gene            41642..43144
                     /locus_tag="PLP50_00175"
     CDS             41642..43144
                     /locus_tag="PLP50_00175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA49986.1"
                     /translation="MKLESFRRRFFELLPRYEWEGTLGQGGIGVVFRALDRELGESVA
                     IKVLSPNLARDENALLARFKREVALNRKIKHPNVARMFDYGIAGDYPFITMELIEGED
                     LWTMIGNRQRFPAAEAVPVVRQIARGVAAIHRLGILHRDLKSQNVLVDRHGAAVILDF
                     GLARGSFDPGLTLASVLLGTPEYMSPEQALGDPLDERADLYSIGVIAWEMLSGQLPFV
                     GDSPVATAMKHVHEPLPDLRELVPGLPADLFGVLLRVLAKKREDRFRSAQELDDALAL
                     CDTGPASPLPGERLLPEAEAFLREVEVAIDSIILPREARPSSRSDAPKTLVTARPRRR
                     QPVVLVVEEDVRGLLKLATTLCMTGCRTLEVRSAEEALETALSRQVDLVLMSTRLPGM
                     DGFDVARILKSIPRAAHIPVLLLAPGPDRAKHAFALQSGAADLLPEPVPQALLEERTW
                     DLLRRRGFSRPSESGPGREKGPTSRPASRPGSRPSPRSSSRSRERSRSRS"
     gene            43185..43772
                     /locus_tag="PLP50_00180"
     CDS             43185..43772
                     /locus_tag="PLP50_00180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPA49987.1"
                     /translation="MSDAPRILALAGSLRAGSYNRRILALAADGARAAGADVTRIDLR
                     DFPLPVYDADLESASGLPSAALALKERFRAAEGLLFASPEYNSAISGTLKNAFDWVSR
                     PAPGEKPLACFKGKVAALCAASTGALGGIRGLASVRLILGNLGVVLLPDQVALPKAAE
                     AFDDDGDVVDPARRKSLMELGAALARATRALRSPS"
     gene            43769..44245
                     /locus_tag="PLP50_00185"
     CDS             43769..44245
                     /locus_tag="PLP50_00185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPA49988.1"
                     /translation="MTDTPAFQDLYPDHWAHCYGCGRLNEHGLKIRSFWDGDETVATI
                     HPRPHHTSVPGFVYGGFIASVIDCHSTGSAAAAAHRAAGGDPAIGPYPRFLTGTLKVE
                     YLKPTPIDAPVELRSTIVEVKERKVVLTTTLTSKGTLCARGEAILVKVPETWEPRA"
     gene            44359..46323
                     /locus_tag="PLP50_00190"
     CDS             44359..46323
                     /locus_tag="PLP50_00190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPA49989.1"
                     /translation="MNRARRTAALAAALALLAVPAVAQPTAYQGHGADSVPPEVLAKY
                     VAPPLPSELSRKIQNLMDVRAPSGGRLSPDGARLYFGWAVTGTAQVWRLDGPNTFPIQ
                     MTGGEDRTSLADVTPDGKTLILSRDRKGEENPGLYLMPADGGPMKVVQHLPKVQTSFA
                     FVSADGKWLYFRSNDVKPSSYAVYRYELATGKKETVFDEPGLWFVVDNRPDDRILLMK
                     ATGSMTFEYWEWDPAKRTKTPMLGTEKPGRYIARYGPKEGQLVVVTPEFGEFARGYLF
                     EVGKWTPITPELSWDVERAGMDDKRTRALYTVNEGGYSKVFGLDAATWKPLEMPKLPA
                     ADQTTFGSSTPDGRFTVFAVGTPTAPPQSWVYDWQEKRLTRWVVPSAPEVDTKSFAVA
                     TLEHYPARDGTKIPAFVRRPKSCDPAPCPVVVLFHGGPEGQATPGFSPMAQLWVDEGY
                     VLVEPNVRGSEGYGKTWLDADNGPKRLDVLTDIEDAAHWARKAFAVDGKAPKLGVMGG
                     SYGGYSTLIAMTKFAGAYDAGVSNVGISSLVSFLQNTAPYRRILRASEYGDLEKDREA
                     LEKLSAVTYIDRLKDPLLLIQGATDPRVPVGEAIQMYEATTKRGVPTGLIIFPDEGHG
                     AQKRENRVLQTGHTIQWFDRHLKGKGLPAK"
     gene            46477..48435
                     /locus_tag="PLP50_00195"
     CDS             46477..48435
                     /locus_tag="PLP50_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA49990.1"
                     /translation="MPVSRRLAPLAALLLALPALGEPQTAYKGHGADSVPAEVIARYA
                     PKPLPPDLARRIQSMMDVRAPGMGIVSPDGSKLFFGWSVTGTAQVWRLDGPNTFPVQL
                     TGGEDRTTIVDVFPDGKTLVLQRDRKGEENPGLYLMPAAGGPLTPIQHLPGIQTFHQF
                     VSADGRWVYFASNDVKPDSYAVYRWNVETRQKEALVTEPGLWSVSDHRSDGTLLLAKW
                     TGSLTSEYWEWSPATRKLTPLLGQDSPEEYTAVYGAAPGQLLVLTPKLGEFRRLCRWE
                     KGTLTPLTGDLTWDVSGFSLDEAKSRVLYTVNEAGYTKLFALDAKTFQPVHLPKLPEG
                     ADHVYAGASTRDGRFTTFGVETATAPRTSYVYDWQSGALTRWVVPSAPEIDTTTFAAA
                     TLEHYPARDGTKIPAFVRRPKSCDPAPCPVVVEFHGGPEGQAQPGFSTWAQVFVDAGF
                     VFVEPNVRGSDGYGRTWLDADNGRKRLDVWTDIEDAATWARKAFAANGKEPKVGVTGG
                     SYGGYSTLVAMTKFAGAYDAGASIVGISNLLTFLENTAPYRRILRTTEYGDPAKDREA
                     LLELSATTHLGKLKAPLQIQQGATDPRVPVGEAIQMYEAATKTGVPTELIVYADEGHG
                     AGKRDNQVLMLGHALRFLETHLKGVPAK"
     gene            48689..50197
                     /locus_tag="PLP50_00200"
     CDS             48689..50197
                     /locus_tag="PLP50_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPA49991.1"
                     /translation="MDIRIDRQSAVPVYLQIRGGIRDLILSGRLPDGFRLPPERTLAK
                     ALGVNRTTILSAYRELKTDGLVEAHVGRGTEVASQQVLKSEAEPAGPLPWRQLARDGG
                     VREPDPLVRDVLALTERHDVISLQAGLPAAELLPLAELRRVQEEILVRKGPEALLHSP
                     TEGVTAFREALCRHMVPRGIRTSPEEVLVTSGSQQGLDLVARTLLSPGDLVVVEEPSY
                     FGALEAFRSARVRLLGVPVDAEGMRTDILEALLARQKPRLVYTLPTFQNPSGAVLSPA
                     RRRHLLELSWRHQVPVLEDDTYSDLRYEGSPLPSLKALDPYGHVIHLSSFSKVLFPGL
                     RLGWIAAPRPLVRQLALVKQTVDLHSGTLGQWIVEGFLSSGLLSAHLARVRAAYRRRR
                     DVLLEALAKEAVPGLSWSRPEGGFYLWCRLPDDVSPASLVAHAAAERVAYLPGAASFV
                     AEPSESSIRLNFTFAPEDDLREGARRLGSALRAAQAEPRRPSRSVVGTPPIV"
     gene            50250..51473
                     /locus_tag="PLP50_00205"
     CDS             50250..51473
                     /locus_tag="PLP50_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPA49992.1"
                     /translation="MTTRFARRMEALKASEIREILKVTQRPEVISFAGGLPAPELFPV
                     KRLAAATQQVFEAEGPRALQYSTTEGHPPLRRKIAERMKATWAAQVTPEQVLVTSGSQ
                     QGLDLTGKLFLDEGDVVLCESPTYLGALSAWNVMRPRWVEVATDDDGMDPTALEAKLR
                     AHPNAKAIYVIPTFQNPSGRTWSLDRRRRFMEVVDRHDVWVVEDNPYGELRLEGESVP
                     SLKSLDTKGRVVALGTFSKVFCPGLRIAWVAAELSVIERLVILKQGADLQTATLSQLQ
                     VNRYLEENDLDADIANIIPVYRERRDAMLAALEKEMPAGVRWTRPEGGLFLWMTLPAN
                     LNARTLLDECVRQNVAFVPGGAFFPNGGNENTLRLNYSNMPIERIREGVKRLAASLVK
                     EMERAGVLQEVPATP"
     gene            51611..52423
                     /locus_tag="PLP50_00210"
     CDS             51611..52423
                     /locus_tag="PLP50_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPA49993.1"
                     /translation="MRRTILATSLALLAFTLSAAAQDSLILATTTSTQDSGLLDDLLP
                     RFEKATGIRVKTIAVGSGEALAMGRRGDADVLLVHSKAAEDEFMAGGFGALRLDVMYN
                     DFVLVGPPSDPAGVRGRPVFEAMTRLAGTGNPFASRDDRSGTHARELDLWKQARVDPQ
                     GKTWYVATGQGMGETARIASEKKAYTLADRGTYLALRKTLDLDILVEGDASLRNSYRV
                     IVVSPEKHPKVRAAEARRFAEWLVSEEAQRAIGAFGKERFGQPLFVPDAKKG"
     gene            52420..53103
                     /locus_tag="PLP50_00215"
     CDS             52420..53103
                     /locus_tag="PLP50_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA49994.1"
                     /translation="MTGVLASLGALLPAPGELLGIVLLSLVVSGVAIVISMLVGVPVG
                     IFLGLHRFPGRAFLVTLVNTGMGLPPVVVGLGVFLLLARSGPLGGLDLLYTPGAMVLA
                     QVVIATPLVVGITLAAIQGLDERLHLQILSLGASRTQLYLKLVKEARLSLVAALAAGF
                     GSIISEVGAVMMVGGNIRGQTRVMTTAIVLETRQGEFASAIVLGVVLLALALGINWLF
                     TWVQQRKPA"
     gene            53100..54212
                     /locus_tag="PLP50_00220"
     CDS             53100..54212
                     /locus_tag="PLP50_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA49995.1"
                     /translation="MSLVALRGVEVVRDGKTILSLDGLALREGELLALLGPTGAGKST
                     LLRVLHLLDRPAAGVIEWRGVPVAWPAPIDLRRRIAMAFQDPLLFSGTTLENVLYGLS
                     VRGVPRSAAREKAEEALRLLRVDHLSHRRARTLSGGEAQRTALARALVLRPELLLLDE
                     PFAALDAPIRRHLLEELTQVVKAHGITCVYVTHEQGEAFSVADRIAVLRRGQLLQAGA
                     PEDVLLRPASRAVARFMQTGNVLAGVVSRRTEALSEVVVGERLLFSRTVLAKGTPVDA
                     CVRREEILVEPPAGGAPAPGLNRFPGAVESVVDHGATLHVRIDAGFPAGPLQALVTRR
                     VAHDLALRPGHPVEATFSASSVHLIPREGSPDDDLS"
     gene            54196..55425
                     /locus_tag="PLP50_00225"
     CDS             54196..55425
                     /locus_tag="PLP50_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPA49996.1"
                     /translation="MTTCREALDLILASAAPLSARPTGLFDALGLVLADRVVSPETVP
                     PFTNSAMDGYAVRHDDLSPASVASPVLLDVLADLPAGSIAAHEVRSGTAIRIMTGAPL
                     PAGADSVVPVESTLSEGNRVRITAPVKRGANVRLAGEDLREGEELLPAGFVLTPASLG
                     ILASVGIAEVAVRPRVRVAILTTGDELVDASAKPGPGQIRDSNVQTMCGQALAFGATP
                     VPIPRVPDTREAVTAALARARAEADVIVTNGGISVGDYDFVKAVLAEAGAREVFWKVK
                     QKPGGPLGFWLWNGAPVFGVPGNTVAAMVCMEEYVRPALRKMMGRSKLLRPEATATFE
                     GDFRKSAPDGKVHLIRVRARRDGDRVVVSPSGPQGSGILSSMLRSNALALVPADATEL
                     KSGDEVLVHLIEEPEDR"
     gene            complement(55654..56787)
                     /locus_tag="PLP50_00230"
     CDS             complement(55654..56787)
                     /locus_tag="PLP50_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA49997.1"
                     /translation="MTRASKSVLLVAAVLVAAASLRPLRAQEPATAAVPAAPKGIALS
                     GSVKKAGVVDGARLAALSRHAVLAKVPGASRYRGTFETEGVSLKELLESAEVAKATND
                     NFDRPLDLAVVVTGRDGKKALFSWGELFLLGDAGAALLVERMRPLVPHHHEAIEDPRF
                     APGSWFGVEAREKLDVSGCAGCHDGGKLGKIDVPRGLCLVPTRDATGRRFVEDVVSIE
                     VRQAGFPAPPRKRDLKDPWVEAPAFVLPDGSSTPLTVAALKAVPRAGGADDGIGLGRG
                     FHGHRSFEGASLAALLKSKLPAGTDPGLLFVVVTASDGYRSLYSGGEVLLSRLPENVL
                     LVDTEGGKPLLRKSGRLESYARADFFVDRSVRNLAEVRVLIAR"
     gene            complement(56849..57091)
                     /gene="thiS"
                     /locus_tag="PLP50_00235"
     CDS             complement(56849..57091)
                     /gene="thiS"
                     /locus_tag="PLP50_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPA49998.1"
                     /translation="MGADPGAPPAADRDTRLAVNGEPLDWREGMTVRDVLQAKNYVFK
                     LLIVTVNGTLAPRGSYDDFVVPAGADVKVVHLISGG"
     gene            complement(57096..59237)
                     /locus_tag="PLP50_00240"
     CDS             complement(57096..59237)
                     /locus_tag="PLP50_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPA49999.1"
                     /translation="MRAAHKVLLEVPFTPSEPERGYNMRSLHVDLTAMKVTEKKVTQQ
                     MKDLFVGGRGFGLWYLWNATTPATRWNDPENEIVIAPGPIAGITQYPGTGKSLVVSIS
                     PLTDIPIDCNVGGYYGPLLKFCGFDAIEIQGKAAAEVVVVVDGPRGVIRIEECPLEPP
                     DSHVLSEIFTHMYATGPEDYKNVSVVSAGKGSDHSYIGCLNFSFWDLRRKAARLKQAG
                     RGGVGTVFRDKKLKALVVRGPVIKADLNHVADRARIQKVGSVINKEIHDLDSSQCNMR
                     RQGTAHLVEVMDAYDLLPVKNFQYGNHPDTKNIASWVWDKRFTQGIPDGCWYGCTMSC
                     SKGADGHVVKTGPYKGDVVTVDGPEYENAAGLAANCGIFDPDWLLESNFYCDTYGVDT
                     ISFGTLCAFVMECWQRGILNAERTGGLDLTWGNAEAQMELLHQMARGEGFGVIAGKGI
                     RQMKRIFAENGWGDPKLMNDIGMEVKGLEYSQYQSKESLAQQGGYALTNKGPQHDEAW
                     LIFMDMVNKHLPTFEAKAEALHYFPLFRTWFGLNGLCKLPWNDIEPANNAEQAEPAKV
                     PEHVHNYCEIASGVTGIEVTKEKIIEQSEKVYNFQRIFNLRRGYGKREHDWPPYRSVG
                     PVTVEEYESRAERYDKQMREEIGVDPEGKTTEEKIAITRQYREDRYSKLCDAVYKRRG
                     WTTDGVPTVATLEKLGIALPELVELVKQHGG"
     gene            59470..60495
                     /locus_tag="PLP50_00245"
     CDS             59470..60495
                     /locus_tag="PLP50_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPA50000.1"
                     /translation="MSPPHGDRYSRQVLFRPFGAEGQARVRAARVLVAGCGGLGAESA
                     SLLARAGVGFLRVVDRDVVELSNLQRQALFDEADVREGLPKAIAAARRIGRVNADVAV
                     EPVVADVGPGNVRSLLDGIDLVIDGFDSFEARFLLNDACVEKGLPWVYGACIAATGLA
                     ALLVPGVTPCLRCLLGEIPEPGSSATCDTAGILGPAAHLAASMQVGRALRFLATREAP
                     PEAELVYADVWEARFTRMAVPRDPACPCCAGRRFDFLSVETSPASALCGRDSVQVRPL
                     VPSRPDFAALAERLRPLGEVVSNDYLLRYRADDVEMTLFSDGRAIVKGTTDPARARSL
                     VARTFGV"
     gene            60507..61235
                     /locus_tag="PLP50_00250"
     CDS             60507..61235
                     /locus_tag="PLP50_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50001.1"
                     /translation="MRPWLVYGFLLALKGLARLFYRVEMTWVGDPPPDRWEHLRLLVF
                     LHHTSLYEPIFLGGAPNGFIRQVAFHGVLPAAEKTTARPIVGMLFRMIAAQVVPISRA
                     RDHTWFEVLRKIDPESMVVLLPEGRMMRTNGLDAHGRPMTVRGGIADVLEAVGEGRML
                     VAYSGGLHHVQAPGERVPRVFRTVRMRLELLAIAPYVREALDGRGPDELREAVCADLE
                     RRRWLHCPVAGPRPWPEPANPIQT"
     gene            complement(61341..62219)
                     /locus_tag="PLP50_00255"
     CDS             complement(61341..62219)
                     /locus_tag="PLP50_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50002.1"
                     /translation="MRRVSLSARCLVPIALFAFVTIAAAGVPPNDTVTVRRSSIRLVA
                     PATVVPYRGGSRCSYLFGPSLQAGPYTFQQQGSGLHLRDERTGEERWIAISELAESAG
                     WPLDPGSHRMLDDTSGMAVGNEELWVGTKGAGILAYSPHAGTWRRYDCKEAMLPGHHM
                     IVSYADDSFVFATNLYHRDLPTTIAEPSLHVYSYAEEAWFEVLTVPSAGGTFTKCPPE
                     MRAILQRAWDHTALANEPAVALKAAGLVHPRVVWVPEEGTYILEFPHRVKELELVTRL
                     RLARADLDGALLRCRE"
     gene            complement(62319..62771)
                     /locus_tag="PLP50_00260"
     CDS             complement(62319..62771)
                     /locus_tag="PLP50_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50003.1"
                     /translation="MREGERRRAGAGVREGTGRSGLFPWPPRDLRWARPVQVALRTLH
                     VAAMAVVLGGLAFDVPDARLHTAGIVTLASGAGLLAVEIARSGVYLYMLAGAVTLLKL
                     ALLVIGQAYPAARFELYLAATLLASVGSHMSSSWRHYSLLERRTLTRE"
     gene            63009..63602
                     /locus_tag="PLP50_00265"
     CDS             63009..63602
                     /locus_tag="PLP50_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA50004.1"
                     /translation="MRTHGTDAPAHGPLSLRAEMKERTGKNTARCYQCGKCSAGCPMA
                     GETRLRPHDVMRLVNQDARARLLADESIWLCLTCETCTERCPNGCDPARTMDALREMA
                     LEASPGSPPRALLAFHRAFLDQVRWTGRSFELGLVADYKLRTGALLQDVATAPGLVAR
                     GKLGFVPHPIDGVKEVRRIFEACEEATRAASGKKEGA"
     gene            63599..64546
                     /locus_tag="PLP50_00270"
     CDS             63599..64546
                     /locus_tag="PLP50_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPA50005.1"
                     /translation="MRVGYYPGCSLHGTARELDESLRAIAPPLDLELVEVNDWSCCGA
                     TSAHATSHLLSVALPARNLALAEEQGHDQLLAPCAACYNRLASARHAVACDAALAETI
                     EDVLGRPFPSTVAPRNLVEVLRARLDLLRERAVRPLTGLKVACYYGCLLVRPSEVCSF
                     DDPEDPVSMEEVVAATGATPVAWRRRLDCCGGAFSVARTGSVVRLSRAILDDARSAGA
                     DAIAVACPMCHSNLDFRQKALVRRGETELPVLYLSELVGLALGLDAGSLGLGRHFVET
                     KGFVERALAPKPAAKPPAGAAKPATPKPAAEATAGKETV"
     gene            64546..66570
                     /locus_tag="PLP50_00275"
     CDS             64546..66570
                     /locus_tag="PLP50_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPA50006.1"
                     /translation="MARIGVFVCHCGENIGRTVDCARVAEELGKLPGVAHAVDYKYMC
                     SDPGQALIKQAIAEKGLTGVVVSACSPHMHERTFRRAAGGAGLNPFLCEMANIREHCS
                     WIHEDREAATDKAIDLARVIVEKVKRNVPLETIRIPVEKRALVIGGGIAGIQAALDLA
                     DGGAEVVLVEKEPSIGGHMSQLSETFPTLDCSQCILTPRMVEVYQHPRIRLMAYCEVD
                     AVEGYIGNFKVTIRQKARSVDETKCNGCGACQKACVVKNVPSEFDERLGKRTAIYVPF
                     PQAVPNIPVIDREHCAYFKGRARGMKKDACGKCKEACARGAIDFAQEDRFVTEKVGAI
                     VVATGYELYSIGKDQPAAGLKGYGEYGYGTVPDVIDGLQFERLASASGPTAGKLLRPS
                     DGKVPKKIVFLQCVGSRDPAKGIDYCSKVCCMYVAKHTMLYRHKVHDGEATVFYMDVR
                     AAGKGYDEFTRRAVEEDGAVYVRGRVSRVYRDGDVVKVLGFDTLNGTPVEIDADMVVL
                     ATAMRPRKGIEKLAQTLSVSYDRHGFINEAHPKLRPVETNTAGVFVAGACQAPRDIPD
                     SVAMASAAAAKVLGLFSNEMLEREPTVARVDAALCAGCFHCERVCPYGAVEHKEVRDR
                     KGNLLKVVADVNPGVCQGCGTCQATCPSKSVELMGFTDEQIYAEVNALAL"
     gene            66581..67084
                     /locus_tag="PLP50_00280"
     CDS             66581..67084
                     /locus_tag="PLP50_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPA50007.1"
                     /translation="MNATLDKTSFEPRLTAFVCNWCTYTGADLAGTSRLQMDANVRIV
                     RLPCTGRIDPLFIVKAFERGADGVIVSGCHPADCHYTSGNYHARRRFAFFKELIEFLG
                     LPEGRITFSWVSASEGQKWREVVNDATARVRALGPFSGYPGTELPAWPAAGAPAPNPA
                     PGGGGER"
     gene            67081..67953
                     /locus_tag="PLP50_00285"
     CDS             67081..67953
                     /locus_tag="PLP50_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA50008.1"
                     /translation="MKELRDLATKLLAEGTVNVVLGWEEGPRGVRPAFATDAAAAGRL
                     VFDPRCTQNLVPYLSPRRTQVKKLGKAAVVVKGCDARAVAGLVRETQLAREDVVVIGV
                     RCGGVIADPASPEPLSPATVADRCPGCNSREPKLADHTVGELPAAPPGESRRDVRIAD
                     LEARTADERWAFWQDELGRCVRCHACREACPMCFCERCVADKTQPQWVDSSPHPRGAF
                     AWNVTRALHLAGRCVDCGECERACPVDIPLGLLNLKLARVASERFGYTATDDPAVPSP
                     VGSFQKDDAQEFIL"
     gene            67950..69005
                     /locus_tag="PLP50_00290"
     CDS             67950..69005
                     /locus_tag="PLP50_00290"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA50009.1"
                     /translation="MNGRTISRSQLAALVDDLVGSGVGVVAPVRAPDGRLDYRPVARL
                     EEAVLDGGLPRLSLKSLFLPPTEPLFSFRKSGPDVAIDSVPTTFPPRLVLGAVPCDAA
                     GVAALDKVMGWDYRDELWFGRREATTIVSVACGKGDASCFCGATGNGPAGTRGADLLL
                     LPLDDGFAVEVVTPKGEALLSAHAGRFGEPPNEADVARRRAGLLEGRVEPVGSLAPVT
                     AWLGGHFDDPVWDGIALRCHGCGACASVCPTCHCFDIVDEPEGVTRGTRRRNWDTCQT
                     ARFTLHASGHNPRSVQDARYRQRVLHKFSIYPERFGELLCTGCGRCSRACPGGMDLPD
                     VLGAIAARAAAPAGGAA"
     gene            69002..69832
                     /locus_tag="PLP50_00295"
     CDS             69002..69832
                     /locus_tag="PLP50_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPA50010.1"
                     /translation="MNLYEPYRVKIAGLVDETPDTRTFRLEFLDPEVARTFDFKTGQF
                     GEYSAFGAGECTFCIASSPTRRGYIECSFKTVGKATTALRDLNVGDTMGFRGPYGNHF
                     PLERMEGSSLVFIAGGIGLAPVRSVIWNALDLRDRFKDVTIVYGARSVSDLVYKRELE
                     EWAARADVRLHQTVDPGGETPDWAGEVGFVPTIVEKSAPSATNAYAIVCGPPIMIKFT
                     LPVLKKLGFADDRIYTTLENRMKCGIGKCGRCNIGPVYVCKDGPVFTAEELAKLPPEF
                     "
     gene            complement(69905..70075)
                     /locus_tag="PLP50_00300"
     CDS             complement(69905..70075)
                     /locus_tag="PLP50_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50011.1"
                     /translation="MAEEQITAGNIAKELGASPAQVKKAIEALKLAPVAKKGACSYYS
                     REQVKKIKGALK"
     gene            70293..70598
                     /locus_tag="PLP50_00305"
     CDS             70293..70598
                     /locus_tag="PLP50_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50012.1"
                     /translation="MTSAIARPPKSLPLHRGGEASAPTTLDDDDGIHIAFVTRAGSPE
                     APILRVVVSWWEEGAWVEKAVDLVDTPPGEVDFPIRDAAPGPAGLSAPSRTPSRPTS"
     gene            71049..73265
                     /locus_tag="PLP50_00310"
     CDS             71049..73265
                     /locus_tag="PLP50_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 Nuclease"
                     /protein_id="HPA50013.1"
                     /translation="MPKLAAPSPSLPPRFRAHPGYSSLSVRDLLDAREAYHVHLSNMA
                     HVVATAIGRYRIRKGDWYETHPPEIPRPRGRKEKKDARDLFNSVTKAWSWPCVLVFVD
                     EWVKREDWKRGKAGRGLEPDQFIPRSLYLPDGRVVPTCVVLLRKSDVRPEAPPPRRFP
                     EPLVGGGYPLLTKVQGTSRVGSVGCLVTDGRQTFVLTNRHVTGEEGDAVFSLLGGKEV
                     RVGRASPLVLGKRRFGEIYDPWPGSRVFVNLDVGLVKVDDVSMLTTQVLGLGPLGSPI
                     DLNPDTISLDLVEKPVRGYGAASGPLSGRICALFFRYHAIGGFEYVSDLLIAPDAGRE
                     THPGDSGTLWCLDTRDAKGQGGLYPIALQWGGQVIEEGPSGGRARGFTLATFVSTVLR
                     ELQLDLVRDWNIGLPEYWGALGHYSIATFACGFVTGRLKTLMGKNVDRVSYPPSRLEP
                     STFPGLSKKDFIPLADVPDYYWKIPGSPGRRATDNPSHFADMDKPLPDAPHAGKTLLD
                     LCIEDPANVDPAVWKDYYERVDDRSKGVLPFRVGLIYEEMVASVAAKDLDRFVCAAGI
                     LSHYVGDACQPLHISSLFDGEPLPGGGKRGAGVHHAYEADMLNANAAALVAGIDAGLA
                     AAAPLPNVSGVREATLAAVDLMRKTFENLSPKAIVDAFADGEDLWDLFGDATIATCVE
                     GARTLAMLWESAWAEGNGDAIPKSKLTARNKDDLVTLYSDEEFIPSLTLKQLIEGT"
     gene            73488..74276
                     /locus_tag="PLP50_00315"
     CDS             73488..74276
                     /locus_tag="PLP50_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50014.1"
                     /translation="MISEILYRSEKSFIFLDPADRLQCEFDEHACIDGDDSMAQASDL
                     VCQARLNHFSRRIRRRAFGLVASGLTRALDTRTAGDKTDFFRDAREFLRFRRREGLQF
                     VYLLSALLSVVAISAVSLLSQLLPIEANGRSILLAAHFGSLGALISISQRSRKIAIPR
                     YASRFYAVAGGGSRIFFGAVFGAALLLLQKAGLVLNLLDSNPYALYAASLVAGFSERL
                     IPELIGQFEKQLRPDESPSVPSGAPSPPPAETGDSAGSPSPSRE"
     gene            74374..75411
                     /locus_tag="PLP50_00320"
     CDS             74374..75411
                     /locus_tag="PLP50_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPA50015.1"
                     /translation="MSRSDQALTETQLNSLWEPKDGHSIDLDLEAGLKLAILSDLHLG
                     DGTASDDFRDNEPALRAALRRYRDDGVRLVLLGDIEDFWKFDLDPVERRYRDTVYKDL
                     RAFPTPALRIFGNHDIEWRQLRDPAAALPDPTRDGFPEGVRLIVDRVPRALLVHGHQG
                     TLDSDRGAWFSRFFVHIWGSLEPFVHALGLHPTNPAATASDVLGDFERTLYSWGKTHG
                     RVLICGHSHRAYFASRSRAEQLRDEQDRLSRSPAPARSSARKSSTLKQLEGLRAELED
                     ERKKGRDIGPLDAGGAPGAWYYNTGCGLYSNGVTAIEIERPAGAASQPVIRLVFWDRQ
                     GSKTTWNTGPL"
     gene            75590..76147
                     /locus_tag="PLP50_00325"
     CDS             75590..76147
                     /locus_tag="PLP50_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50016.1"
                     /translation="MANAAAARRPDTVPANDFPRTLDQIASAAATPPAPAEPSPGPGR
                     LAVGSSGSTDTALHVLFGYIPTEVLTLYVAVLAALHRESEVTRADWLAFWIFLAATPV
                     IVWLVYGAKLKALQKPLPLRIGTWPVWEMFAATAAYSAWAFALPNSPFSTYPWYSSAL
                     SGLAVLIVSTLLGLIAPFLQRPLAT"
     gene            76194..77114
                     /locus_tag="PLP50_00330"
     CDS             76194..77114
                     /locus_tag="PLP50_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPA50017.1"
                     /translation="MPTYRVLSLDGGGIRGLVTTVLLERLAATPGLENLLDKTDLVSG
                     TSTGGLLALAIANGLSLAEMRDMYVKKGPQIFDDSWLDDLVDLGQLRGADYDTGPLRR
                     ELRRLFGDTTLGKLRKKVLVTAFDLDNEDPAHRTWKPKLFHNFQGKGSDGALPAWKVG
                     LYTSAAPTYFPSVDGFVDGGVYAGNPSMCALAQTQDARYSPTPPMNQVRLLSLGTGTN
                     LQYVAGTTLDWGYVQWARPLISLMLDGTAGIADYQCSQLLGDRYHRLAPTFPNDVNIP
                     LDDVKKIPYMLDFAAKVPLDVTIDWLKRFW"
     gene            77728..78102
                     /locus_tag="PLP50_00335"
     CDS             77728..78102
                     /locus_tag="PLP50_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50018.1"
                     /translation="MKRLTLLSLFAAAFAVFCSLPSRAEDTTIRFLDGSVAKPGDTWI
                     ADIKQKDGSVLAYRKNDDDIAKLVAALRSIPKVKKEMLTTAFWAARECAKTAGNQCSS
                     GSCPSGKSCKQTGTTFTYCSCQ"
     gene            78243..78671
                     /locus_tag="PLP50_00340"
     CDS             78243..78671
                     /locus_tag="PLP50_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50019.1"
                     /translation="MIKRRRTPTATGRLSGPLASDFDRSVRDLAAAVDALVPVAFALA
                     WSPTENSPVFIQVVAVNGNPRTPPSGEVDPASPYLLALGAFPDGAEITISWVIRAFAD
                     LHGIRSFARTGSRPWERLDDKVPLQYQGQWKGESRLRVTP"
     gene            78668..79702
                     /locus_tag="PLP50_00345"
     CDS             78668..79702
                     /locus_tag="PLP50_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50020.1"
                     /translation="MRHTRLLCVIACLAFPATSTAQPVDGPVFEPPTSRTDSEDWPPA
                     QSRLPADAGPGFLPATPDLLAAHFSLKREGTAIAITSHPFRVGSQRALWGIRTTGGYD
                     SGEKTTGFGLGYRFEWRWLFAAQWTSRTVDAHRRAAEMTSSVSDEKRAAEVDRAMLRI
                     ADLETLSLIPILTVGYQIDLFPARLGGTDASSMARGLASLNLSWRYGVYVDVESNLSI
                     GHQRADETSPLVGLLSAGAVLLVTIPELLGATAYDDYYRRNLLQRGVSVGPAFSYQEC
                     RADAAEQAACPKGLIRDRLVGAAIELRVADKVTPRFTVGKRRLWTRKSDTSTGEDFTE
                     GSVSLTYTFR"
     gene            complement(79884..80882)
                     /locus_tag="PLP50_00350"
     CDS             complement(79884..80882)
                     /locus_tag="PLP50_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50021.1"
                     /translation="MRNARYAAQPTVTKLAEDRAEQLKRDPDKVAQELEDRLRADLRK
                     TGDFSRIHPLPRSGADVPDDLETRLVVLSAEHPYGKEPSSPAEVAAKAILASRGSAPR
                     LYQNTLVFLAADRVRLEDLDEALRKFLAWESIVADTNTLNLDPRQVRQAETQKQAAAF
                     AYAESFDEAASRYRGLECGQVVAVPAESTGLLVKADVARKEIDEETQAAAAAAAATGA
                     GSASASGTAGSAVAGQASSSAPGASPAPATLPTGPVPPRRYHGTVRLDPARAGRDASR
                     IADEVIAHLAGLQGATVTVTLEIEATIPGGASEQLVRTVTENGRTLKFESFGFEEE"
     gene            81060..81737
                     /locus_tag="PLP50_00355"
     CDS             81060..81737
                     /locus_tag="PLP50_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50022.1"
                     /translation="MSPDRPGWAPPAPSADGFPEAVKLIVDNAAKAILVYGHQETVDS
                     DRGARFSRFFVHIRGSLEPLLHALGLLPANTDATASEVLGDLERTLYLWIGTLGRSVA
                     HGHSYRAFVAFQSRAECPRDEQDRPSRSIAAERSVAVNATNGIALEDLRVELENEPKR
                     GRDIGPLNASGAPEASYQDAGCDLYSGSTTALEIGRPQDTSAPPVFRLVFWDRRGLRA
                     TSRTGQL"
     gene            82335..83432
                     /locus_tag="PLP50_00360"
     CDS             82335..83432
                     /locus_tag="PLP50_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50023.1"
                     /translation="MAKPPAPADGGRSSGHQTTKPYCRNLPRSAPALPPAVTADPARA
                     AAIVLGRSKWVNGTVLHYAFFSGSSRYSVPHGQADAVRDAFAKWKAIGIGLEFKEVPL
                     LAQAEVRVGYSLVDGTSASGVGRDILNISLNEPTTVYGWDLTSPYGRGTALHELGHVL
                     GMEHEHQNPYAGIKWHEQAVYDALAKPPNRWDRSTTYHNILEKLSPQQVQGSRWDPDS
                     IMEYEFEPGLIDEPQEYDVNGLTPPGTLSADDMAWVRKWYPPVDHTLPLLAPFESRAV
                     DLAAGEQVDFAVAPAVSQKYTIETKGATDTLLVLFEDVNGEPVYLSGDDDSGEERNAM
                     ITYRLLAGRRYIVRVRLYYPGRSGKTHLMIL"
     gene            83705..85099
                     /locus_tag="PLP50_00365"
     CDS             83705..85099
                     /locus_tag="PLP50_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPA50024.1"
                     /translation="MKVKELRSILNASKAQWAIPADIADDTELEAISRPFAMGGMPPA
                     PGAPTARSPRMRRPADAAVALWQPGGNRLTRPFVTALPRTWDWRDVHGHTWISPPKNQ
                     GGCGSCVAFGVSAALEAHQRIEANDASLPFDTSEANLFFVNERQCNLGDPRYGWSIPP
                     ALDYLVDQGACPEEDYPYRDVNQNAELVLGTQGTWKIKGYDSTSNVRQAKRWLCEDGP
                     LVTRFTVYQDFMTYWSLGANGVYTHHAGSTLGGHAVAVIGYSDEQSCWICKNSWGSTH
                     GNDGCFRIGYGQCGIDDRMYLIQDVYDVLTRDEIAYNPRTLRIVDEGARGWLLTDGVS
                     RMKILDSKEDARNALCVARRYTRQGFVGRDNRRANRIDYITEYWAGNSGLPHEPLTRE
                     DAIPYDPSKVVAEDIDADGWRLREGSHWMLLADDLNDALAVLQIVERYTKMCFIGRGN
                     HRPDRKSYIMTYWE"
     gene            complement(85302..88667)
                     /locus_tag="PLP50_00370"
     CDS             complement(85302..88667)
                     /locus_tag="PLP50_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HPA50025.1"
                     /translation="MALTNQERVGKAMDLLKQGLAPFVERELTNAHGDRARAEALKLV
                     GEEPNVAKRPLADWDVAALLKVMRGAWNDVFGRVLGRAERTLVQELGDWRNDWAHQKA
                     LSSDDADRALDSMARLLTAISAPQADEVGKMKLELRRLVYDEQVRSEKRKAGASLIDV
                     AAGGTLKPWREVVTPHADVASGGYQQAEFAADLWQVHLGEGTPEYRDPTEFFRRTYLT
                     ESLRALLVNGIRRIYGKGGDPVVQLQTNFGGGKTHSMLALYHLFSGVSAKSLPGVEAI
                     LAEAGVKGVPAAKRVVLVGNKISPGNPVTKPDGTVVRTLWGELAWQLGGKKAFAKVKA
                     DDEKATSPGDALRELFKEYGPCLVLIDEWVAYARQLHDQRDLPAGTFETQFSFAQVLT
                     ESAKLAKNCLLVISLPASDTTGSPHTQTDDVEVGGIRGREALDRLRNVVGRLEASWRP
                     ATSDEGFEIVRRRLFEPLEGPASFTQRDLTARAFGDTYRAQSAEFPPECRTADYEKRI
                     QAAYPIHPEIFDRLYADWSTLAKFQRTRGVLRLMAAVIHSLWEKGDKNPLILPSMIPI
                     DDGRVRDELTRYLSDNWVPILEKDVDGPGSLPLKLDGDVPNLGKLHATRRVARTIYLG
                     SAPTTSAAHRGLEDRRVKLGCVMPGEPPAVFGDALRRLAAAATYLYQDGPRAWYSTQP
                     TVTKLAEDRAEQLKRDPDKVAQELEDRLRADLRKTGDFTRIHPLPRSGADVPDDLETR
                     LVVLSAEDPYGKEPSSPAEVAAKAILASRGSAPRLYQNTLVFLAADRVRFEDLDEALR
                     KFLAWESIVADTNTLNLDPHQVRQAETQKQAADGAVTARLPETYQWLLAPGQANPQAP
                     VKWEATRLTGTDALAVRASKKLKSDEWLVTTLGSTVLRKHLDDVPLWRGDRVAIRQLV
                     DDFARYLYLPRLLGPEVLAHAVTDGVRLLTWQVDTFAYAESFDEAGPRYRGLKCGQVV
                     AVSPESTGLLVKADVARKQIDEETQAAAAAAAAGAGSASAPGAVAGGVSGRASSSAPG
                     ASPVPATVPAGPIPPRRYHGTVRLDPARAGRDASRIADEVIAHFAGLEGADVTVTLEI
                     EATIPDGASEQLVRTVTENGRTLKFESFGFEEE"
     gene            complement(88684..90024)
                     /locus_tag="PLP50_00375"
     CDS             complement(88684..90024)
                     /locus_tag="PLP50_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50026.1"
                     /translation="MNELSASDLKSVLLAKLKESLTEPIPEGTPRRLHGAASLPGKVS
                     SVVGVRRSGKTTFVHQERREQLAAGAAREHVPYVNFEDERLSEMTAAQLGLLLEEYGR
                     LFPEARERARVLWSFDEIQLVKGWERFVRRLLDEGRNDILVTGSSAALLSREIATALR
                     GRAWEVPIYPFSFAEALLFQGRPIPARPGFLGGAERGRIERAFLDWLSTGGFPEVQKL
                     DAASREKMLSDYVDVAMLRDVMERHGVGNVVGLRWMVRQLLGNAGALFSVEKFHAALK
                     SQGVAIARDTVHQLLAWLEDCFLVRPVWMESASERQRMVNPRKCYPVDTALIALFDRT
                     GRANTGHALETAVLVELQRRQLAVTYVKTPEGHEVDFLARGAGGQADLIQVCADATDP
                     ATAERELRALVEAGRIHPKAKKRLLTLTSDGRPAEVPRGVEVQPAYEWMLSAPD"
     gene            complement(90076..92973)
                     /locus_tag="PLP50_00380"
     CDS             complement(90076..92973)
                     /locus_tag="PLP50_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HPA50027.1"
                     /translation="MSREISRKKLIEVALPLQAINEASAREKSIRHGHPSTLHLWWAR
                     RPLAAARAVIFAQMVDDPSSCPDLFPTEKKQEKERQRLFKIIEELVKWENTTNEEVLE
                     AARAEIWQSWRRACADNADHPRAKELFDRHKLPAFHDPFAGGGSLPLEAQRLGLEAYA
                     SDLNPVAVLINKAMIEIPPKFAGRPPVNPEARANGELIAREWKGAQGLAEDVRYYGKW
                     MRDEAEKRIGHLYPKVEVTAEMAKGRPDLKPYVGKKLTVIAWLWARTVKSPNPAFRKV
                     DVPLASTFMLSTKEGKEAYVEPVIEDGGYRFTVKVGKPKKAEEAKNGTKLARGANFRC
                     LMSGTAIEGDYIKAEGKAGRMGARMMAIVAEGERSRVYLPPTSEHEAIARKAKPDWRP
                     EGDVPARLTGGTCVPYGLSVWGDLFTPRQLVALTTFSNLVQDAKERVERDSVTAGPPY
                     CNGHQREGEGGPIGYPEAVASYLSFVLSKSIDGSSTICRWMTQRDSLFSTFSRQALPM
                     TWDFAEVDLLADCTRSLSESTAWTAESVAALSVSEGTKPGTAFQADCATEVAADGRVV
                     STDPPYYNNIGYAELSDFFYVWLRRTLQRVFPELFRTLAVPKIGELVAAPYRHGGVEQ
                     AEAFFLDGMTSAMSRLSAQAHSAFPVTIYYAFKQAESPDDLGTSSTGWETFLDAVIRA
                     GLGVSGTWPMRTERQGRSVEVASNALASSIVLVCRPRPADAPTATRRELVNALKAELP
                     AALAHLQRGNIAPVDLAQAAIGPGMAVYTRYSKVLDAEGKPVSVREALALINQVLDEA
                     LAEQEGDFDADSRWALAWFEQHGFGEGEFGVGETLSKAKNTSVAGLVDAGILKSSRGK
                     VRLLKPAELSPKWDPESDTRLTEWEMVHHLIRVLEAEGEAAAAALAAKLGAKAEVARE
                     LAYRLYTICERKKRAPEALSYNALVQSWPEIVRLAREGGAREAGQADLFSS"
     gene            complement(93037..93381)
                     /locus_tag="PLP50_00385"
     CDS             complement(93037..93381)
                     /locus_tag="PLP50_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50028.1"
                     /translation="MLEHARREGGLDLGPLLPFWNAVCDRYRSERYDAQGELFGDEGK
                     ARLDAWNRFVYHELFPALCREDEIVRNVLRAEALLPSRSPRGAAEALVRYAEDMVLPE
                     GLPLRDPANPDD"
     gene            complement(93527..94132)
                     /locus_tag="PLP50_00390"
     CDS             complement(93527..94132)
                     /locus_tag="PLP50_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HPA50029.1"
                     /translation="MKPIYIAPIVEGHGEKEAVPALLYRLASAAGIERSVRVNHPIRV
                     KAGSFLHDGAYLKRHATLAAEKAAQAGGLVLVFLDCDDGCPATLGPDLLRRVRAVRSD
                     VEWLIVLAQREYESWFLAAARSLRDRHGLPSDLDRPPQHESIRDAKGWLGDRMAGGYD
                     PLTHQFAFTKHFDLDEAAGNRSFRRFRDRIGTVLVSRTETC"
     gene            complement(94129..95352)
                     /locus_tag="PLP50_00395"
     CDS             complement(94129..95352)
                     /locus_tag="PLP50_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA50030.1"
                     /translation="MNLTGPLLRRVTLRNYKSISACRVDLGPLVFLVGPNGAGKSNFL
                     DALRLVSESLSTSLDHALRDRGTIKEVRRRSGGHPNHFAIRLDFSLPNGRVGHYSFRV
                     AARSAGGYEVQTEECRVAEGAALEGGDHFRVDRGKVTSASFSPQPAAIPDRLFLVAAS
                     GTPQFRPVFDALSRIRIYNLIPRELAAMQKPDPGDLLRRDGSNAASVLQRLTPAVRER
                     VDEHLSRIVAGLSSADPKTLASQETIEFRQTVRGQKHPWRFLASSMSDGTLRAFGVLL
                     AAFQAASRREGAIPPLVVGVEEPEVALHPAAAGVLLGALREASRTCQILVTSHSPDLL
                     DNPDIPIDALRAVDNRDGLTRIGPIDAAGRNVLREKLFTAGELLRQNQLAPDETALQD
                     VDDERQLELFELQSR"
     gene            complement(95438..96682)
                     /locus_tag="PLP50_00400"
     CDS             complement(95438..96682)
                     /locus_tag="PLP50_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50031.1"
                     /translation="MVRRHEPPVLPRAAGAAIERALKIMPVVVVLGARQAGKRTLAQT
                     LPALARRPYFSLDDIDLRTHADADGEAFVQRAPLPVLGEVQRSPDLLIAVKRAVDLDD
                     QRTPGMFVLTGSANLLLMEKVSESLAGRAVYVTLWPFTRRERHGEGATGPWSELLAAS
                     FSRWPQVLDAHPGPEESWSSAARLGGFPAPAHRIADESLRSSWYGGYVQTYLERDLQT
                     LRAVESLADFRRLMGAASLRIGSLLNQAELGRDIGISQPQVHRFLNILEVSYQAVRLP
                     AYSANRTKRLIKAPKLYWCDTGLALHLAGETEPRGAHLENLVLVDLLAWRDLTVGRPE
                     VLYWRTASGQEVDFVVETPRRLLPIEVKASSRVTRSDVAGLEAFLDEYPDLADGGLLL
                     YGGRETYKVTKRVLVAPWWRVI"
     gene            complement(96723..100298)
                     /locus_tag="PLP50_00405"
     CDS             complement(96723..100298)
                     /locus_tag="PLP50_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPA50032.1"
                     /translation="MKLEDLQPQGAVRGILPDGLVTVVSTEWHGSDALTLIYRGPTGG
                     VAEEVLYRHDEPRLTLVEAGRPWSFDGDGALYRLVAEAHRIRLAHLFDPVLAVHTSLV
                     DPLPHQITAVYEEMLPRQPLRFLLADDPGAGKTIMAGLFIKELIARGDLKRCLIVCPG
                     SLAEQWQDELYRRFHLPFEILTNDKLEAARTGNWFLENDLAIARLDKLSRNEDVQARL
                     AAPDAGWDLVVCDEAHKLSATFFGGEVKLTKRYKLGQLLSGLTRHFLLMTATPHNGKE
                     EDFQLFLALLDGDRFEGRFRDGVHQVEVSDLMRRMVKESLLKFDGRPLFPERIAYTVP
                     YKLSDAEARLYKDVTDYVREEFNRAEKLANDKRAGTVGFALTILQRRLASSPEAIYQS
                     LRRRKERLEKRLRELELQQRGGALPAVIAAGPDLDPDDVDDLEEAPENEVEEAEEEIL
                     DQATAARTIVELKTEIATLARLEGLAAEVRRSGRDTKWRELSRLLGEIFTPAAFGESV
                     AEESAPYGSGPLPKPVPSPRQKLVVFTEHRDTLNYLARQIGTLLGRPQAVVVIHGGMG
                     RDERRKAQETFLHDPMVQVLLATDAAGEGINLQRAHLMVNYDLPWNPNRLEQRFGRIH
                     RIGQTEVCHLWNLVADETREGDVYRTLLEKLEEARSALGGQVFDVLGKLQFDGKPLRD
                     LLIEAIRYGDQPEVRARLTRAIEQTIDRPHLEELIDERALAHDAMDTSRVARVREEME
                     RADARRLQPHYVESFFLEAFRRLGGTVRQREPRRYEVTHVPAPVKNRDRQVGMGEPVL
                     ARYERIAFEKDLIAPAGEPLAAFLCPGHPLLDATLDLTLERHRDLLRRGTVLVDERDP
                     GTRPRVLFTLEHAIQDASLLPSGERRTISRRMLYVELDADGDARHLQYAPYLDYRPLA
                     PGEPAIDALFGRPEMAWITRDLEKTALGHAIATVVPGHVGEVRDRRLSWVEKTRAAVK
                     DRLTKEIAWWDHRAEELKAREHAGRPGARLNSQEARRRADDLQSRLQKRLAELDREAQ
                     LSALPPVLLGGLVVVPMGLLVAMQGKPAAALAASPDTQASAARAREIVMAVERGLGFD
                     PIDRETEKLGYDVESRVPGTGRLRFLEVKGRVSGADTITVTKNEILYSLNKPDDFILA
                     IVEFLPDGAHGVHYVRKPFQREPDFGACSVNYAFGDLIARAEAPA"
     gene            complement(100930..102345)
                     /locus_tag="PLP50_00410"
     CDS             complement(100930..102345)
                     /locus_tag="PLP50_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPA50033.1"
                     /translation="MIAAHPDDFRLLVYLEGELDELEAARLKRHLAVCPACAAACRDI
                     ERLDRTLKSTLPDLEEALAEPALPQGDPFRVQPDGTPRGAARPAGPTEIPAVLAASRE
                     AIGVKSRLLDAAAAPEEDLRSALEALDLLGLRDRYGLGYALDEALYGMVDGPARWDRF
                     GRLSADRVLAARADGRALDAPAERAYPLDDLLGLSLLVSGTACNWTGEFLRGGRDLRR
                     AYGAFARGSVSERRLASVELAEAQRRAFLDRADEALHLAGRARLTFQSLGMEDEAARA
                     AAAHAIALSYLARDEEALEEFRSILPVLAQHERWSPYVSTLNGIGFCLLRLGRVEEAR
                     REYARALRLVGREARPAVHAFIRANLARTLFEGKRYGDAARAFDGAARLFASQGATGD
                     ELTMLLLRVEALARSGSAVLARGLLGEVSRRVRALGAVDAAVLESLEAALRGDLPDVD
                     LLEVLRTRAEVQISERLRRAV"
     gene            complement(102342..102785)
                     /locus_tag="PLP50_00415"
     CDS             complement(102342..102785)
                     /locus_tag="PLP50_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50034.1"
                     /translation="MESYLRHRFRFSAPEVEDLMQDVLLELMRHEEPVREAEGLAFRI
                     LQLRSLQFLRRRRARPELPLETGAGAEGNLEAPSGTDPDVLVLLRQTLAQASPACRRL
                     IRAHYLEGRTLKETAESLAYASPNVVWTLLDRCIRRLRAAVGAAR"
     gene            complement(103025..103756)
                     /locus_tag="PLP50_00420"
     CDS             complement(103025..103756)
                     /locus_tag="PLP50_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA50035.1"
                     /translation="MTGPSADRISLFFPVYRDEKTVERVATKARRVLDELAAEWEIIV
                     VNDGSPDRAGEIAEELARQDPRVRVVHHGANLGYGRALRTGLAAARYGWIAFTDGDDE
                     YEVDDLRKLWRLRDRYDLVLTFRYAKRYGSARIFISWVYNNVLRWLFRTHYRDISCGL
                     RLVRREVASELPLRSTSPFIGAEIAIKTMLKGFRVGEVGIQTFPREFGKGSSTSLANI
                     LATMRDMRAVYREVFSSEYDRPLNH"
     gene            complement(103753..104451)
                     /locus_tag="PLP50_00425"
     CDS             complement(103753..104451)
                     /locus_tag="PLP50_00425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA50036.1"
                     /translation="MTTARVDERRSPPGEVLAELYRRRFPPAELEEMRAVWRVLVRDF
                     FGPRIPERATVVDVGAGPCLFINEVRAARRIAVDASPALAEAAGPGVEPIVDDDLDLP
                     GIPDAAADTVFMSNFLEHLASPAELLRALAAARRVLKPGGELLVLQPNFRLCPRRYFD
                     FVDHSLAVTDRGLVEGLRATGFEVVELRVRFLPFTSKSRLPKWPWAVSLYLKLRPLQW
                     LLGGQTFVRARVRG"
     gene            104508..106334
                     /locus_tag="PLP50_00430"
     CDS             104508..106334
                     /locus_tag="PLP50_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50037.1"
                     /translation="MSGTVPVLAAAATNAVFAAALLLGLLFLGAATVPRHLRPSDGPT
                     RFAAFLSLGASLFGATTWVAGALVGTRAFGPLWVALGLLALLGGARSGLSLLARLARR
                     LTALLRASPLLSAALAVAALPLAFPLSIPLVDNDGIRYHVALPKLYLLTGEVFLYRWD
                     VTGAYPQLGNMLALAGLAAGRADVAKFVNAGFLAASAALLALALHRGRASRAASLAGP
                     LLLLAAPVVGVVAGSGFIDHVALFHLAAALVLVSRNGSPLLSGLALGAALATKLTLAP
                     GAVAVGLAAALLRPRGERWRAFLVVPAAAAAVLAPFAIRNALALGDPFYPVLTLAAGR
                     PTPGVSRTVFTHFTSFNAGNAGPLGIVWTTPSSGADDETAGIHNVAGLVLLLFGFRDR
                     LVRLASLLVLPFLAYAALSAPPTRYLLPLLWGLSLVAAAVLGRLREGRLAWLAIPLAL
                     PGLVLSWSYQARNFGAADYLLGRVSREELLARTVPGYRATSFVNGLPPGGVMAADLPG
                     PLYLDRPWIVSGFINEAPLTLWIREGDDSDRLLGRLREHGIRWLLATPGYGGGTPLSL
                     LPYAPDPSKGHVMAALRAKLRLVATVDGVDVWEVPGATSARP"
     gene            106581..107738
                     /locus_tag="PLP50_00435"
     CDS             106581..107738
                     /locus_tag="PLP50_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA50038.1"
                     /translation="MFEPWFTKLEQVHEAKSREERDAVYAFRYKIYVEELGRELGGVD
                     HVRKVVCDGDDEQECSVLLYTGRLDDISATVRLRIWQPGRIPRHEFEMLSMSKFPGIE
                     ERTISELGRLMIRPTLRGKLLLPSLVRSVYERGVVAGVDLGFCYCAPGLVPHYRKLGF
                     RPYAAPLVPTVDGLEVPLVGIASDAKYLKQMGSPVASLVSRFYGTGPGKRPPLDLSTL
                     GDLLDPGGSSVELERDQVWQEVQDGLLQDAGASSILDGLSEAAVRKLTAKGFLLDVAR
                     DTLVTRAGHGQREMYIVLEGTLEVLSAEGRRLAVLGRGDLFGELAFFRDEGRRTASVR
                     AASDCHLLVLRRHFLTELGKSDPDVALQLHFNLGRILSERLVRVERKLAAG"
     gene            107751..109973
                     /locus_tag="PLP50_00440"
     CDS             107751..109973
                     /locus_tag="PLP50_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA50039.1"
                     /translation="MPESGRTREAGALLAESETFGSLAEPLRSRLAGAMRRRTVAAGE
                     VLLRQGEVSDSLFVIAAGRAEVRLRRDGGPEVAIDTIGPGGAVGEMQLVVGGVASATV
                     VAAEETQVFLLRRQTFDALCDEFPELLEAVARVAGRRVRRHRMLEVLPSLFGPLGDGV
                     LAAIEGQVEWLTLRSGEVLFHKGDPGDAWYVVTSGRMAVVEPASGGQADRLLAEVGQG
                     EALGELSLLTGEPRSATAYALRDAELVRFPMDRFAELVSAWPQVTNGILRTLARRLVT
                     RGDASRRPTEAGLTLALLPATPGVDVAAFAERLAVALSRFGPTLQASSSRLVEIGIQS
                     ETASASGSHPAWIRVGAWLEAQAASHRFLLLVADATPSAWSARAVGHADQVILVADAN
                     GDPSPGALEHALRPSAPARRQPRRLLALLHRDGSRPPRDTARWLDARAVDAHVHVRLD
                     REDDVRRVARGIAGRGVALALSGGGARCFAHLGVVRAMREQGIPIDLVTGTSAGAMSG
                     YLVATDSSDDDLRRSALRFHQARPFGAYTLPLFSLIRGERLSRALADECGPTRIEDLW
                     LPFVAVSSNLTRKAVELHTRGPAWAALRASCSLPGIVEPWIRDGELLVDGGLIDNLPV
                     GVARERIVGRVIAVDVSSAQRLTFAGSAYPSPWTRLVTRFGRRQGHPSPPGVFDVLLH
                     SMLLASLANVERMRLDADVCLRPELAGFGMLSTGLHAQIIDAGYRHAREHLKSFDPSL
                     "
     gene            complement(110114..111241)
                     /gene="alr"
                     /locus_tag="PLP50_00445"
     CDS             complement(110114..111241)
                     /gene="alr"
                     /locus_tag="PLP50_00445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPA50040.1"
                     /translation="MSLLYATHARIHLGNVRDNLRAIRAAVGAERKVLVAVKANGYGH
                     GAVEVSRMAEATGTADWLGVATVPEGLQLREAGLRLPILKLGPTFPAEMEASVAAGIT
                     LAVCSREEADAAAEAARAAGRPAAVHLKVDTGMGRVGVTQEDAPALALHLAALPGVEL
                     GGVFTHLPVSDAADPAWTREQIARFRAAAAAVQEAAGRRLLVHCANSGAVLGHEAAWL
                     DMVRPGIMIYGFRPDPATPDTIPLKPGMSLVTRVSFLKRVPAGTSIGYGRTWVAPRDT
                     FIATIPAGYADGFNRLFSNRGRVLIGGRSYPLVGRVCMDQSMVDLGPETDVRVGDEVV
                     LMGRSGDAEIRCEEWAEKLGTITYEVTCQVNPRVTRVYDPY"
     gene            complement(111238..112317)
                     /locus_tag="PLP50_00450"
     CDS             complement(111238..112317)
                     /locus_tag="PLP50_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPA50041.1"
                     /translation="MFIRTPRLEIYPDRIAQNARAVIALCHEHGAQVAAVTKVVCAHP
                     AVVNALVAGGADMVADSRIRNLQAMADTGIDRPLMLLRIPAPSRAADVVRCANVTLNS
                     NLGTIELLSEAAVLHGVTHQVIVMVDVGDLREGVWPDRAIEVVRGASKLRNVEVIGLG
                     CNLACYGGVIPTKANMAALVEVRNRCREETGLDLNVLSGGNSANLPLLASGQMPWEIN
                     HFRIGEAIVLGRNVIDRSPWPGTRQDTFKMVAEVVEVERKPSMPIGERGQDAFGGTPE
                     FEDRGVRLRAICNLGRQDVVVDGIEPLLPGVTVLGGSSDHLILDVEDAAENVRVGDEL
                     EFWPGYGALLALSTSPYVQNVVIRG"
     gene            complement(112326..113678)
                     /locus_tag="PLP50_00455"
     CDS             complement(112326..113678)
                     /locus_tag="PLP50_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPA50042.1"
                     /translation="MQIEVLTLEVGSTITKANGFVRLAAGGFDHVAQGFAPTSVGAGD
                     VGIGVDAALADLRARSGLDPSGAETFVSSSAAGGLRMTVHGLTMSMTARAAREAALGA
                     GGIVKLVTAGELESWELDEIRAISPNLVLLAGGVDHGEKGIVLKNAEKLAALVAGGLR
                     VPVLYAGNAALRRPVTEIFRLAGVELHVAQNVFPDVDVLNVDPLRRLVHEAFNRHIVN
                     APGMQRLAGLTRHEVLPTPGAVLRGAELFAEVAGDCLVFDVGGATTDVHSVTEGSADW
                     HARLVEPEPRAKRTVEGDLGVYVNARNVVALADEPDGEERLADLSALPVTERQKALTR
                     WLCDRAVSIGARRHAGVVSDLYTPTGRKQVVKGKDLTAVRWVVATGGALTKVEGAAEC
                     LRAVCTGAGRHLLPDPSATILVDAEYRFSALGTLAHAYPDDVRATFRRWVEAHAPARP
                     "
     gene            complement(113761..>114678)
                     /locus_tag="PLP50_00460"
     CDS             complement(113761..>114678)
                     /locus_tag="PLP50_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPA50043.1"
                     /translation="AGSLVPRDADYLAPVCDHFGTNHLPAGAAKACDPIGGCTLCDPS
                     KIVFIDELDAEDNCHRRLEKTLPYRKGTAIKPEAEWAGDGTVLVQLFVPENEDIAPVY
                     AVEMARKMGLVDPEVINTMVLHPAEGCFVEVKGKVAFDVDRTTLKIPEKETILPEQEL
                     RDAVKKIGLKVVAGTVGEDEHSVGLREILDIKHGGIEKYGVKYHYLGTSVPVGKLVDA
                     AVETGAHAILISTIISHNDVHRAQMKKLAELCREKGIRDRVVLVAGGTQVNREMAKET
                     GLDATFGRGTKGIHVVNAMVKTLRARGAA"
BASE COUNT        16205 a        42044 c        39452 g        16977 t
ORIGIN      
        1 ccgaggagga gggtcccgag gagccggagg ttcaccgcgg ggttgtgcgc gcgggagggc
       61 cggaagtcaa ttcgggaggg cccgccgggg cggttcgtcg aacggcgcgg gcgcggcgcc
      121 cgccctacgc tcgccccgtg ctccgccgcc cgctcgtctc caggctcggc tgcggagccg
      181 ccatactcgc cgccgtcgcg ctccccgccg tcatgctgac tccgctcgtc cgctcgatga
      241 tcttccccgc ctcgcaggcg ccgttcccgg agccgcggcc gcaggacggg tggagcgtcg
      301 cgcggtacga gacggcggac ggcgttcggc tcgcgggcgc gttcttcccg gcgccgtcgc
      361 cggacgcccc ggtcctcctc tacttccacg ggaacgccga ggcggcggcg cacaacctcc
      421 cgctcgccga cgacctccgc gcccgcggct tcggcgtctt cctcatggag taccgcgggt
      481 acggcggact cgccggctcg ccgtccgagg aggggctcta cgccgacggc gaggcggccc
      541 tcgccgagct cgggaggcgc ggcatctctc ccgagcgcgt cgtcctcgtc ggccgctcgc
      601 tcggctcggg ggtcgccgtc gagctcgcga cgcgccaccg ggtcgccgcc gtcgtcctcg
      661 tctcggccta cacgtcgctc gtcgacatgg ggtggatcgt cgccgggccg ctcgcgccgc
      721 tcgtcgtccg ggaccggttc gactcgctgg cgaagatcgg gcgcctcgag gcgccgctcg
      781 tcctcctcca cggggcgctc gacgacgtcg tcccggtggc gatggggcgg cgtctcgcgg
      841 cggcgcggcc cgacgcgcgg tgggtcgagg tcccggggac gagccacaac gacttcccgg
      901 gcctcgcggc gctcgtcgcc cgcgaggtcg acgcggctct cgccgacgcc cggaacgcgc
      961 cgccgccgta gccgcctccc ccgggcccga gctccgcggt ccgccacgct acgatcctcc
     1021 gccatgggaa ccgtcgagcc cccgcccgga cagccggccc catacgccgc cccggccgtc
     1081 gccgcctccc cgggccgctc gccgtggacg atcgcgctcc tcgtcctcgg cgccctcgcc
     1141 gcgttcctct ggttccggcg gccgagctac ctgcccgaca tgccgctccc ctcggcctcc
     1201 gccgagggcg tctccgccgg cccggacgcc tcgccggttt tctgccgcgg ctccaagggc
     1261 tgcctcgtcg tctacgtcgc cccgtggtgc ggcccctgcc gctcctccct gccgggcgac
     1321 gtcgccctcg ccgaccacat cgccccgatg gggtacgaga ccgtcttcgt cgtcgggatg
     1381 gacgacgtgc gcgcctgcct cgagatgacg aagtcgatcg gccgctccgc gcggatcgac
     1441 tccgagcgga agtgggcgcg ggccgcgcgg atcagcggcg tgccgcactt cctcgtgacg
     1501 actccgaccg ggaagatcat gaagcggcag gcgggcgcgt acctggcccc gccggaccag
     1561 gtcgcgcagc tcctcgggat ctcctgacgg ccgcccagac ggggcgcggg tagagttctt
     1621 ccgtgtccgc gctctccacg aggctcgacg atccgggagc aataccctac atcctctggg
     1681 acgatccgat gaccgtctcc gagctcgaag agcgactgcg cacggcgtcg gagcccgagc
     1741 ggctgaggct cgtcgccctg atcctccgcg aggcccggga cgccgacgtc tggcgattcg
     1801 tgacgcccga ggaggtcatc cgactctggc cgcggctcgc cggacgtctc ggcctccgct
     1861 tcgcgttctg ggagttcctc ctcgaccgct ggaagcggct cgggctggtc gaggcgtagc
     1921 ggatgtcgcg tttgagccgg cttcaggagg acgtccttca cggcttcttc cgacggacgc
     1981 gctcgttctt cctgacgggc ggcgcggcgc tcgccgcctt ccacctcggg cacagggcga
     2041 ccgacgccct cgacctcttc acgtcgtccg ccgatctcga cgaaggggtt tcggcgctcc
     2101 gctccgtcgc cgaggagctg ggcgcctcga tccaggccct gcggacttcg cccgccttcc
     2161 ggcgattcct gatcggcgtc ggcgccgaga gcgtcatcgt cgacctcgtg cgcgaccggg
     2221 cgcctcagat ccacgtcgag aagctcgacc gtgacggcgt cctcgtcgac ccgcccgcgg
     2281 agatcctcgc gaacaagctc tgcacgctcc tgtcccgggg cgaggtacgc gacctcgtcg
     2341 acgtcctgtt cctggagagg gccggattcc gcgcggagga cgccatgggt ccggcttcga
     2401 gcaaggacgg gggcctcacg cccgcgcagc tcgcatgggt cctgtcgcag atcaccgtcg
     2461 ggcccgatgc acgcattccg gccgggatca cgcctgacga gctccgggcc ttcctcgaga
     2521 accgcgtctc ccgcctgacc cgtctcgcct ggccgggcgc gcccgcctcc tagctagttc
     2581 acaggctctt cgtcgtccgg gacgagcgtc ggatcggcgg cgaggcgggg ccgctcgggg
     2641 gcgggggaaa gcgacgcgtc gacgctctcg tcgaggaccc agcgctcgta gagcgtcgcg
     2701 tcgagctcgc ggaggaactg gctcggctcc gtgacgatca cgacgccgta ccggtcgaac
     2761 gccgtcaggg ggtggacgag cgtcagctcg tccttcgcgc gggtcgccgc gacgtagaag
     2821 aggcgccgct cctcctcgag cccctcgggg gtcttgcagg cgcggaggtt cgggaagcgg
     2881 tcttcctgga ggccgatgac gaagaccgcc ttccactcga gccccttcgc ctggtggacg
     2941 gaggagagga cgagccgctc gtcgtcctcc tctcccgcga cctggtcctc gccggagggg
     3001 tcgccgtaga gcgtcacctc ctcgaggaag cggccgacgt cgtcgtaggc gagcgcgaac
     3061 tgcgcgagcg cctcgaggtc gtcgaggcgc gcggagtggt tcgggaactt ccccttcgcg
     3121 acgtcgccgt agccgccgtc ctccacgacg tagcggatcg cctcggcggg cgaggagacg
     3181 tacgacggct tcttcagcgt ccgcatcgtc tcgcggaagc ggaggaggcc cgcgcgcgcg
     3241 ccggccggcg ccttgccgag ctcgagcgcg aggaaggccg cgagcggctc gtgggagtcg
     3301 gagacggcct cccagaggac ggccgccgtc cgctccccca ccttcgggag gagcttcagc
     3361 gcccgcttga acgacatctc gtccttcggg ttcacgagga gcttcaggtg cgcgaggacg
     3421 tccttgacgt gcgcctgctc gaagaaccgg agccccgagc ggacctcgta cgggatgccg
     3481 cggcgggtca gctcgatctg cagctccagc gcctggaagt gggcgcggta gaggacgccg
     3541 atctcggaga ggggcgtccc ctcgtcgcgc agctcgagga cccgctgcgc cacgaactcg
     3601 gcctgctgga ggaggtcctt cgcgccgacg acggccaccg gcacgccgct ttcgcgcgtc
     3661 ggaacgagcg tcttctcgta gcggcgcgag ttgtgcgcga tggaggcgtc ggcgagccgc
     3721 aggatctcgg gcgtcgagcg gtagttcgtc tcgaggcggt agaccgtcgc gcccgggtag
     3781 cggtcgggga acccgaggag cgcctcggcg tcggcgccgc ggaaggagta gatcgactgg
     3841 gcgtcgtcgc cgacgaccat gacgttcttc gcttcggagt cctgcgcgac gagatcgacg
     3901 atctccgact gaagccggtt cacgtcctgg aactcgtcga cgaggatcgc gcggaagcgg
     3961 aacgcgagcg ccttgcgcac ctccgggtgc gtctcgagga gcctcctcca gttcaggagg
     4021 aggtcgtcgt agtccatcgc gttcgcgagg accttccgct cgaggtagcg gcggatcacc
     4081 gccttcaccg gctcgagctg cgagaggagg tgcgggtagt cgcgcccgat cacggcgtcg
     4141 agcttctgcc ccgtgttcgc cgcgaaggag acgatcgccc ggaggaggtc ccctttcggg
     4201 aagcgccgct cggtgatcgg gatgccgagg tcggaggtgg ccgactcgag caggtccttc
     4261 gagtcctcgg aatcgaggat cgagaagttg ggtcggtagc cgaggagctc ggcgtgaggg
     4321 cgcaggaggc gcgcgccgac ggaatggaac gtgccggcgg tcatccgggc gaggtcggcg
     4381 ccgaggaggc gctcgaccct ccgggtcatc tcccgggcgg cccggttcgt gaacgtcagg
     4441 aggaggatcg cctcgggggc gaccccgtcc tcgacgaggc gggagacgcg gtagacgagg
     4501 gtccgggtct tccccgagcc ggcccccgcc acgacgaccg tcgggccgtc cggggcgaag
     4561 acgaccgcct gctgctcggc gttcagctcc cggcggtagt cgatccgata gctcttcgcc
     4621 ggccccgcct tccggaggac gtagcgcttc ggggcggggg gcggggcctc gggcggcatc
     4681 ggggcggagt atctcgcgcc ggagcgcgtc tcggctcagg cgcctccggc cgtcctccgc
     4741 ccggcgattc tggtagcgtt gccgcatgag caagccgatc cccgagggcc tctgggagct
     4801 cctcccgctc cttttccgtc ctcacccctg gcacggcgtc tcggtgggcg acgaggcccc
     4861 cgacaaggtg accgcctaca tcgagatcgt cccgaccgac ccggtcaagt acgagctcga
     4921 caagaccacg ggctacctga agatcgacag gccgcagaag ttctcgaacg tctacccgtc
     4981 gctctacggc ttcatcccgc agacgttctg cgccgaggag aacgcggcgt tctgcgcgct
     5041 gaagacgaag cgcgagggaa tcgtcggaga cggcgacccg ctcgacatct gcgtcctctc
     5101 cgagaaggac atctcccacg gcgacgtcct gatggacgtg atcccgatcg ggggcctccg
     5161 gatgatcgac aacctcgagg ccgacgacaa gatcgtcgcc gtcctcgagg gggacgccgt
     5221 cttcggccag ctccgggaga tctaccagct gccgccggcg ctcgtcgacc ggctcaagca
     5281 ctacttcctc acctacaagc aggcgcccga ctccctccag ccgcacaagg tcgagatcac
     5341 gcacgtctac ggccgcgagg aggcgcacga ggtgatccgc cgcagccggg ccgactacga
     5401 caagaggtac ggaaagctcc gcgagctcct gaccgccgcg ctccgggcct gacacgcccg
     5461 ccacgccccg tcgcatggag agagacctcg agagcctccg cgcgctcctc gccgatcgat
     5521 accggatcga cggcgagctc ggccgcgggg gatacgcggt cgtctaccgc gcctggaacc
     5581 tccggctcga gcgcgcggag gccctgaagg tcctctcgcc gaagcggggg gacgacgacg
     5641 atttcgcccc gcgcttcctg caggaggtgc gcctcgcggc gggcctcgag cacccgtcga
     5701 tcgtcaaggt ctacgacttc ggcgaatcgg gggggggcga tctggtactc gatgcagctc
     5761 gtcgacgggc agacgctcgc cgtgcggctt cgccacgccg gcgccttctc cgagccggag
     5821 gcggcgcgcc tcgccgtccc gctcctcgac gccctcgcct acagccacga acgcggcatc
     5881 gtccaccgcg acatcaagcc ggagaacgtc atcctcgacg cggacggccg gccgtacctc
     5941 accgacttcg gcatcgcgaa gtcggccggg tcgctcgtca agacccagac cggctttctg
     6001 atggggacgc ccgcctacgt ctcccccgag caggcgcagg cgaagccgct cgacggccgg
     6061 accgaccttt acgccctcgg cgtcacgctc taccggatcg tctcgggccg ctacccgttc
     6121 gaggcgcccg acccgctggg ggccgtcgtc ctccgcctca cccagccccc cccgccccct
     6181 cgtcgaggtc ctcccttcgg tcgaccccgc gttcgcggcg atcgtcatgc gggcgctcga
     6241 gcgggacgcg gccctgcgtt ggaagtcggc tcgcgcaatg ggagacgcct tcgacgactt
     6301 cctcgccggg aagccggcgg gaagcgggcg gctccgggtc ccgcaggctt ccgtgtcgcc
     6361 gcaatctgca gaaacggtca tggcgcccgc cgaggcttcc gaggcgaccc gcctcggacc
     6421 agccggacag gtcggcgtcc cgctcccggc tccgacaccc gaccctcccg cctcgacccg
     6481 cgggaggaga cctcgaaggt tcggcgtcct cgggctcgcc ggcgcggtcg tcggactcgt
     6541 cgtcctcctc ggggtcgtga cgctcctcgt cggccccggc cgcgatcgcg agccggaccc
     6601 ctcccgaacg accccttcgc cgcggtcgac cccctccgct ccggccgcgg ccgccccgac
     6661 gcccgccgtc gccgatcggt cggtcgccga gcctccagag gcgcacgcga cgccgacggc
     6721 ggcgccgaac gccgaatcgg tgccggccgc gccttcccgc cgtcgggcgc cggccgagcc
     6781 tcttccggag cctccaccga tccgccgccc cgtcacgacc cccgagcgcc tcgacccgga
     6841 gccccccttc gccgggccgc ctcccgccgc ctgctcgggg gccagggtga ggatcggctt
     6901 cgctgtggac gagaccggaa gaatcaccgc cccgcgcctc ttcccctcgg aggcgccgcc
     6961 ggagtgcggc cgcttcgtcc tcgacgcgct ccccgcctgg aagtggaggc ccgcggtgga
     7021 cgcctccggc gttccggtcg cgtcggcgcg gctcatcgtc gaaatccagc tgccctgagc
     7081 tcgcgcgact atcatcggct gatctcgatc cgaagggaga tgcccagatg acacggtccc
     7141 ggcccctcct tctcctcgtc gtcgcctcga ctgcgctcct cccggcctgc accccgaaga
     7201 acgcccttcg gatggcgatc gaggtcgtct cggagccgga cgccgcgatc gtctccttca
     7261 aggggaagga cgtcgggacg acgcccacgg ccctccgcgt gaggacgttc gacgagctga
     7321 tgacgatcgc ggcccgaaag gacgacgcgc cggtcgccga gaagcggatc cggatcctct
     7381 cccccgacca cgcccagctc gtcttccgct tcggcacgga gccctcggag gtggcgaaac
     7441 gcctcggact gacccgtgtc gtcgtcttcg actactcgga gaaggtctcc ttcgacgtca
     7501 gcaagagcga gctgaagccg gaagggcttc cgatcctcga ccgccaggcc gagatcctcg
     7561 cccgctactt cccgaacgtc gacgtctacg tctgcggcca caccgacgcg accggaggcg
     7621 acgacttcaa cctgcagctc tccgtcgacc gcgcgcgggt cgtcgccgac cgcctctccg
     7681 gccagggagt ccctcccgag cggctgaaag tccagggctt cgggaaggag ttcccgaacg
     7741 acacgaacga gacgcctgcc ggccgcgcga acaacaggcg gacggagctg atcctgccgc
     7801 agtagcggag cccgggacgg acggctcttc cgtcgctctc tccgcggctc cctgtgagag
     7861 actggccggt ggaggagttc agaccatgag catcgatgcg atccgtctgt tcggcgaagc
     7921 ggtccgcaag gacccggcac tgctcgagaa ggtcaaggcc gtcggctcgg acgtcgacgg
     7981 gatggtggcg ctcgcgcgcg aaagcggctt ctccttcacg gccgaggagc tccgggaagc
     8041 cgcgggcgcc ggcaagggcg agctctccga ggagcagctc cagaagctca gcggcggggg
     8101 ctcgtgggtc caggtccaga ccgtcgcggt tcacgtggcg gtggtcggct agccgaacga
     8161 atcccgtacg gggggaccgc cgcgaggggc tgtcctcttc gatgacaacg aatctcgaca
     8221 cccgatacgg aggaggaatc ccgatgagca aggaagccat tgcgaagttc caggaagccg
     8281 tccggacgca gccggccctt ctcgagaagg tcaaggccgt cgggtccgac gtcgacgcgc
     8341 tcgtcgcgct cgccggcgag gccggcttcg tcttcacgcg ggacgagctg atggacgccg
     8401 cccaggcgaa gaagggcgag ctctccgagg aggagctcca gaaggtggcg ggcggcacgg
     8461 ccgtcggcgt cctgacggtc gcggtcgcgg tggtcgcggt cgccgcgacc tgacggccgg
     8521 atcccgatgc cgggccgcgg ggcggcggga ccggagccgc cgggtctccc ggcgtgctcc
     8581 ggtgaacccg tcgccgcatc gacggggccg tcgacggacg gctccccgtt cgacttccat
     8641 cgccgacacg tcggccgctt cagcccttcc gagcgatcgg agctggccca cacgaagcgg
     8701 ttcctcgagt gcgtgctcgg ggacggggag ttccgcgccg cgctcggcgc cggcgaggag
     8761 gctcgcctgc aggcgtgccg tcagcgcggc ctgcaggcga agccggccga cctcgagccg
     8821 ctctggcgcc acggcaccgt gttcgaggtc ccgaccgagg aggccgggcg ctacccggcg
     8881 gtggcgctct ggatcgagtg gctcggcggc ctcctgcgct tccgcgacct gctccgcgag
     8941 cacgggagga cgacggcgtg ccaccctgcg ttcgacgcgt ggcgcctccg ccagatcgcc
     9001 cggggccggc gggagctggg ccacgcgagc gcggcgatca cacatccggt gatcgcgttc
     9061 gagctgtcga aggggtgcac cttcggctgc tggttctgcg gaatctcggc cgcgagctac
     9121 gagggccatc tcccctacga gggcgcggga cgcgcgctct ggcgcgacgt cctcgcgtcc
     9181 gcgaccgact tcctcggacc ggcgatgcag acggggacct gctactgggc cacggacccc
     9241 tcggacaatc ccgactacac gcggttcctc gacgacttcc gcgaggtcac cggcgccctt
     9301 ccccagacga cgaccgccgg cgcgatccgc gacgtgcgct ggacgcgcga ggtgctcgca
     9361 caggcggcca ccggtccggg gacgccgacc cgcttctcgg tcctctcgaa cgggatgctc
     9421 cggcgcatcc acgagacctt cacggcggag gagctcctga ccgtggagct ggtcctccac
     9481 aacccggagt cggccctcgg cgtcgctcgc tccgggaggg cgctcgggcg cgaggcgcgc
     9541 cggatgagcg acacgcaggg gacgatcgcg tgcctctcgg ggtacctcgt caacatggtc
     9601 gaccgcagcg tcgcgctcct cagcccgtgc aacgcctccg aacggtggcc gctcgggtac
     9661 cgggtccacg cgcggggccg cttcgcctca gcggaagagt tccgagcgtt cctggaggag
     9721 accgccgccg aatgcatgcc gctccacctg cgcggcgagg acccgctcgc gttccgtccc
     9781 ggtatcgagc acgccgcgac cgcggacggc ttcgcgctgt cgtcccgctt cacgaggcag
     9841 gaattctcgg gaggagcgac ggcccgccgc ctcggtccgc tcgtcgcggc cggaggctct
     9901 accgtcgggg aggtgatcgg cacgctggcc gccgagggcc tcgacgtcct cgcggcgacg
     9961 acgctcctgc agagcctcta cgactgcggc gtcctcgacg acgagctctc ggggagctgg
    10021 ggctgaagct tggacgtcct cttcgtcaac atgccctgga tcggcctggc ccggccgtcg
    10081 atcgccctcg gaacgctgaa gtccgtcctg tcggccggcg gggtgcgctc ggaggtcgtc
    10141 tacgcgaacc tcctcttcgc gtccgagatt ggggtcgagg cccaccaggc cgtgtcgctc
    10201 gtctacgcga accacctcct gggggaatgg ctcttctcct ccgtcgcgtt cccgggcgcg
    10261 tcgagcggct ccgacgccta cctcgaccgg gtcctctcgc gcgacatctt ccacaaggcg
    10321 tactggaagc cggagcgggt ccgcgagctc gtcctcgcgg cccgcgcccg cgcggagccg
    10381 ttcgtctcgc gcctcgccga ggacgtcgtc gcccgccgcc cccggatcgt cgcgtgcagc
    10441 tcgacgttcc agcagcacgt cgcctccctc gccctgctcc ggcgggtaaa ggagctcgac
    10501 cccgggatcg tgacgctcct cgggggagcg aactgcgaga cggtcatggg gcggacgacg
    10561 caccgcctgt tcgaccaggt ggacttcgtc gtctccggcg aggcggacga cgtcctcgtg
    10621 cccctggtcc gggccatcga gcgcgacggc cgggaggtct cgcccgagga gctccccgtg
    10681 ggcgtcctcg ggccggccca tcgccggagc ggctacccgc gcgtcgcgga cgcccagggc
    10741 gcggacgaca cgccccgggc cttcgcccgc gacctcgagg cgatcccgcc gcccgactac
    10801 acggagtact tcgagacgtt ccggtcgctc gacgcgctcg tccccgccgt cacgcccaca
    10861 ctcctcgtcg agacgtcccg cgggtgctgg tggggcgaga agcgcacctg ctcgttctgc
    10921 ggcctcaacg gacgggagaa cgcctatcgc aagcgctctc ccgggcgggt cctcacggag
    10981 ctcgacgacc tctcgcggcg ctactctctc gcgacgttcg agaccgtcga caacatcctg
    11041 ccgatgtcgg cattcgagga cctccttccg gcgctcgtcg aagcgggaag cccctatcag
    11101 ctcttcttcg agacgaaggc gaacctgacg cgcgaccagg tccggctgct cgccgaggcc
    11161 ggcgtgcggt ggacgcagcc cgggatcgaa agcctgagca cggagctcct ccggcttctc
    11221 gggaagggct gcgaggcgtg gcagaacatt cagctgctca agtggtcgcg gcagttcggc
    11281 atccggctca gctggaacct cctctccgag gtccccggcg accgcgacga gtggtacggg
    11341 gagatggccg agttcctgcc gctcctcgag cacctccagc cgccgtccgg cgtggcgccg
    11401 atccagtacc accgcttcag ctcgtatcac cgcgaccccg cgcgcttcgg gctgcggctc
    11461 cgccccgccc ccggctattc cgccgtctac ccgctgcccg aggaggagct cgccgacctc
    11521 gtctacctct tcgaggacgg gtggcgggac accgtccgca agagcccggt gctgaagatg
    11581 ctcctcgacg gccccggcct cccccggctg cgccagcggt tcctcgcctg ggccgggcgc
    11641 tgggtcgaag gcccggcacc ggtcctcgcg gcgacgtaca cggagagcgc gatccacgtg
    11701 agcgacacgc gctccgtcgc gccgaaggcc gagcggaccg tcaccggcct cgatcgcctc
    11761 gtcctcctcg aggccgaccg cgccccgctc gagaagggcc tcgtcgcccg cctcgtcgcc
    11821 gacggccacg aggcgaagtt cgtggccgag tccgtcgagc ggctcctttc gcagcgcctc
    11881 gccctgcgcg tcgacggccg cctcgtcggc ctcgtcctgc aggacccggt ggcgccgctc
    11941 ccggcgctcg aagaaggacc cttcggccga gagctgccgc gcgagaggcc ggccgagacg
    12001 tgatcgtcac accgcacgcg gcgacgctcc ccggcccctt ccgcctcgac ggcggcggcg
    12061 tcctcgagga cgtcgaggtg gcgtatgagg agtacggaga gccgtgcccg gggcgcgaga
    12121 ccgtactcgt ctgccacggc ctgacgaccg acgcccacgc ggccgggcgc cacacgtgga
    12181 tcgaccgcag cgccgggtgg tgggacgcgg cgatcgggcc gagcaaggcc gtcgacaccg
    12241 accgctatcg ggtcctctgc gcgaacgtcc tcggcggcgc cggcgggacg accggaccgg
    12301 ggtcgatcga tccgcggacc ggccgcccct tcggccttcg ctttccggtc gtgacggtcg
    12361 gcgacatggt cgcggtccag gcggccctcg cggaccacct cgggatcgag cgcttccacg
    12421 ccgtcgtcgg cgggtgcctc ggcggcttcc aggtcctcga gtggatggcg cgcttccccg
    12481 agcgcctcga acgcgccgtc gtcatcagcg ccaccccgcg gacctccgcg cacaacctcg
    12541 cgttcttcga ggtcctccgg caggcgatca cgagggaccc gcgcttcgtc ggcggcgact
    12601 actacggcgg cgaggtcccc gccgacggcc tggcgctcgc cgcgatgttc ggcgccgtcg
    12661 tctggctcgg gcgcgagacg atggagcgaa agctcggcgt caccctcctc gagggaacga
    12721 agccccgctt cacgctcggt cccgagttca cggcccaggc gttcctcgcg cagatcgccg
    12781 agaacgcccc cgcccgcctc gaccccaact cctttatcta cctgacccgg gcgaccgact
    12841 acttcgacct ggcgcgcgac gcggggagcc tcgccgccgc cctggccggc ttccggggac
    12901 ggagcctcct cgtgagctac gcctccgact ggcgttatcc gccctccgga gtcgacgaga
    12961 tccgcgtcgc gctcgaagcg aacggcgccc cggtccggca cgagacgctc gagagcgaat
    13021 tcggccacgg cgcgttcgtc tacgacgtcg agggcgtcgg cgacctgctc cggcgcttcc
    13081 tggccgactg atcccggcgc ctcgtcagac cggccggaag tggtcgatcg tcaggagcgg
    13141 ccggccgacg gccgcggtgt cgaggtcccg ggagaacgcg gaggcgagcc ccgcggcgcg
    13201 agccgccgcg cggaagacgg gggcctcgac gaggagctgg cccgagaggg cgtggcaggc
    13261 gtcgacgagc gtgagcggcg cgcgcccgat cgcggcggcg atcgcctccg gcgggaccgc
    13321 gtgggcctcg acgacgacca tcccgtggcg cgccgtgaag gggcggtagc gccggaagag
    13381 ctgaacgagg tcgagctcga gccggcgcac ggcgatccgc tcccccgcgg cgtcgacgaa
    13441 gacgccgtcc gggtcgccga acgtcgggag gccgtcgtcg agaccgggag ggacgaagcg
    13501 gcggttgtgg atcacggact tgctgacgtg caggacgtcg tgagggtcga ggcctctctc
    13561 ccggagcgtc gcggcgatcg cgtcggggtc ggcgatgtcg ccgcgcgcgg acccgccggg
    13621 gacgccctcg gcccggagcc ggcgctcggt ctcgacgcgc gcgacctcgt tgtactcgac
    13681 gccgaccatc cggatcggca cggcgtcgag acgccggccg cgcgccgtcc gctcgcggat
    13741 cgcgcggtag agctcgacga ggagcgtgcc gtcgcccgag cccgtgtcga cgatcgcggc
    13801 gggctgcgac tcgagcggct cgctctcgaa cagggggagg accgccgaga ggagcggctc
    13861 gaggcaggcg cgccggaaga cgacgccgga gaacgcgatg tcgagcgcgc ggtcgacgtg
    13921 gcgctcgacg ccgtcgtggt cgtcgacgcc gctctcccgc gccgccggcg cggtgaagat
    13981 ccgccggccc gcccgaccga gcgtcgggag gtagctcgcc gcgtaccagt actgtggcgc
    14041 cacctccgag acgacacgcc cttcgaacgt gagcgaggtc cgcccggcct ctcgcttcag
    14101 ccaccccctg gccgcgagga cctccagcgc cgcggagacc gcccgacccc gtccgggcag
    14161 ctcctcgtcc gggcagccgc gcgcaccggc ctccgcgaga cgggccagcg caccgcagcg
    14221 gtcgagcccg gtcaccgcgg ccgcgacgag aggcccgtgc cggtgggcgc gcgcgcgcct
    14281 ttcgatgagc tcgccccccg gctcgatcgc gagatcgtcg gcgcccacga ggggacgcgc
    14341 ggccccgtcg cccgcgaacg cgcggagcag cgcccgggcc cggtcgatcc gcgccgccgc
    14401 gccgctgtac gtcccgagcg tccgcagccg gtcgaggccc gtcgccgtca gccggacgcg
    14461 gctccggccg ggctcccctc cgccctcgcg ggagacgaag ccctggtcgg cgaggaggcg
    14521 cagcgcgacg tcgaggagtc cgggccgagc ccccgcccgc gaggccagct cgtcggcggc
    14581 gagaggcgcc gaagcctcgc ggagcgcctc gaagagcccg cggctctcca tcgcctccac
    14641 gctcgtgccg atggccgcgc cgtcgaggtg ccagcagacc gtccgccaga ccgaggcgta
    14701 gagaccggcg aggagcggat cgcccggccc cgtcacgacg gcgggcccgc cgagccgtga
    14761 cggccgccgc gcgccgccgt cacgcgatct gcctccgcgc gaggctcgcg aactcgcgtc
    14821 ccgaggccat cagctcgtcg aaggtccccg cctcggcgac cctcccgtcc accaggacca
    14881 cgatgcggtc ggcgttccgg atcgtcgaca gccggtgcgc gatcacgacg cgcgtggcgc
    14941 ggagctgctc cagcgtccgg ctcacgacgg cctgcgtgtg gttgtcgagc gcgctcgtcg
    15001 cctcgtcgaa gaggaggatc cgcgggcgac gggcgatcgc ccgggcgatg aggatgcgct
    15061 ggcgttgccc tccggaaagc ccggcgccgc cgacgccgac catcgtgtgc aggcccatcg
    15121 gcatcgcctt cacgtcgtcc tcgatgccgc agagccgcgc ggcctccatc gcctcgtcca
    15181 tcgtcatccg cgaggtgccg gcgatgttgt cgtagacggt tccgttgagg agctggctgt
    15241 tctggaggac cgcgccgagc tggcggcgga ccgccgcgag gtcgagcccc gcgaggtcga
    15301 gcccgtcgta gaggacggcg ccggtctccg gcgactcgaa acccaggagc atccggtaga
    15361 gcgtcgactt gccggagccg gacggcccga cgagcgccac gaactctccc ggctccgccc
    15421 ggaacgagac gtcctcgagg acgagcggac cgtcggcgtg gtaccggaag ctcacgcggc
    15481 ttacctcgac ggcgccggag agctcgcccg ggtccgcgcg gcgcgacgag ggctccgggg
    15541 gcgtctcgag gatcgggcgc gaccgcttcc agaccggcac gacgtccagc accgggaaga
    15601 ggagcgagag cgtcatcgtg agcgacgaga cgaccgcgcc gagcgcggcc aggaacgcca
    15661 cgaacgtccc gagagacaag ctggtcaggc tgcggtccgt ggagaggccg acgagcgcga
    15721 agacgaaccc cgtcgccagc ggcggcagcc cggccccgag gacgacgacg ccggcgagca
    15781 ccttcctcac gccgaggtcg agccgcttct gctcggcgaa ggcgcgcgcc caccggttga
    15841 aggcgcgctc ctcggcgccc gccacccgga gcttcgcgat cccttccgtc agctggagcg
    15901 tcagcccctg gatgcgcccc gcgacctcgt agagggccac ctggtggtgc atcaggagcc
    15961 ggaagaggac cgccgcgacg acgaccgcga gcgcgaccgg caggagcgcg aaccaggtca
    16021 gcctcgggct gtaccagaag aggagcccga cgttgaagag gccgaagagc gccgagagga
    16081 cgctcatcga cgccgcgccg gtgaggcgtt cctggacgaa cccgaccgcg agggcgcggt
    16141 tcgcgaggtc gccggtcgcg aagcggcgga agaaggaggg gggcagggcg agcagccggt
    16201 cgaccaggcc ggcctgcacg gcgccgccgg cccgcccgct gatgcgcagc tgggcgaacg
    16261 actggacgac ctggagcccc gccaccgcga accccgccgc gaccagcgcc gccaccaggt
    16321 acgcgagctg gcttcgcgag tcgctcggga tgacggagcc gaacaggagg ccggtgccga
    16381 tcggcgtcag gagggccaga gcccccgcga cggcccccag cccgaagagc cttccgaggt
    16441 ccggcccgga gccgaacccg aggaagcgca gcagggcgcg gcggcccggc gtccccggcg
    16501 ggagcgcgcg gtagaggacg tgcgcgtgcg gctcgagcgt cgcggcgacg gcggcgtcca
    16561 ccctcgtcct cgctccccgc acggggtcca cgagaacgtg acggccgggc ccgtccggca
    16621 ggatcgccac cggcctccgg tcgtccttcg cgaaggtgag cacgggcccc ccgtcgcgcg
    16681 tccaccaccc gtcccgcagg agcacctctc gggtccgcac cccggagtcg gcggcgatcg
    16741 ccgcgaggct ccgaggctcg ggcgctcccg cgggggcggg tcggatcgcc acgcctcccg
    16801 cctcggcaac gagccggcag gcgacggcga gcgcgtcgtc gccggcgctc gcgatcggga
    16861 gcgacgtccc gaggacggac gccagccgtt cctcggcctc ggagcgcagt gcccggacgc
    16921 tcctcgcgcg ccggaggagc tgcgcgcggg cctcctcggc ggcccgcgcc tgccggcgct
    16981 cgaggagctc gacgacggag cggccgaacg aggcgagcgc gttcggcagg ccgccgggcc
    17041 ggccggcgac ctcggccgtg ccgagccact ccagctccgc gtcccgcgcg accttcagcc
    17101 agaagccggc gcagagcggc gccgccgctc cggcgccgaa cggcgcgacg cccggctctc
    17161 cgtacggcag gagctcgccc gcgcggaccc cgacccagcg aacgcccctc tgggccacga
    17221 gccactcccc ctcgccggcg acggccgcct cctcggccgg cgtcctcgag gtccgcggag
    17281 gcagccggtc ctccgagagc gccgcggcga gcgcctgcag ccacccttcg acgagcgaca
    17341 cgaccgcccc tccgattgcc gcgtccccag cgagagggag tagtcgcgcg agctccacct
    17401 cgaagagcgc cgtctcgggc ccgggcacgg cgcgcacgcc gccctcgccc tcgagcgccg
    17461 gcagcgcgag gagagcgtct ccggcctcga cgcggcagac gtgccggcgc gggccgcgcc
    17521 cctctgccgg ctcgaagaag acgtcgacgc tcccctcgcg gacgagccag gcgcgcgtcc
    17581 cgccggcgat cgagaacgcc gagaggggcc ctccgcgccg tgacgggccg gcgcctccgg
    17641 ccgccgtcgc gatcgcgtcg agcgcggcgc ggggatcgat cgcgtcaggc atcctggatc
    17701 agcctcgagt aggccccgcc ggagaggacc agctcgtcgt gggtgccgcg ctccacgacc
    17761 cgtccgcgct cgaggacgac gatctcgtcg cagtcgcgga tcgtcgagag ccggtgcgca
    17821 atgatcaggc agctgcagcc ccggcgccgg agcgcgtcgt cgatcctctt ctcggtcagg
    17881 gggtcgagcg cggaggtcgc ctcgtcgagg accaggaggc cggggttggc gtgcagcgcc
    17941 cgcgcgatct ccaggcgctg ccgctggccg ccggagaggt tgcgccctcc ctcctcgacc
    18001 gcgccgccgt atcccccggc gcgcgccgtg acgacgtcgt ggacgcacgc gtcgcgggcc
    18061 gcgtcgacga cgtccgaatc cgggaccgtc ccgtcccaga gcgtgaggtt ctcccggaac
    18121 gtcccctcga agaggaagat ctcctggtcc accgacgcga ccgaggccgc gaggacctcg
    18181 cggggccgcg cggtgcgggg cttcccgtcg aacaggatct cgccgctcca cgggcggtag
    18241 agacccgcca cgagcttcgc gaccgtcgac ttcccgctgc cggagacgcc gacgagcgcg
    18301 acgcgccccc cgggccggac cgtcagggaa aggtccttga tgagcggctc ggccagcggg
    18361 ctgtagccga acgtgacgtc gcggagctcc aggtgccccg agagcttcgg cgggtcggcc
    18421 gcgcttgccg cgtcgagcgc ggggacctcg gggccgtacc actgcaggtc gaccgggttc
    18481 tcgaggacgt cgtcgagacg ggtgaggtcg ccgtcgacct gctgaagctg cgaaccgagc
    18541 gcgacgagcg tcccgatcgg gaggaggaac gcggccacga gggcctggaa cgccacgagc
    18601 tcgccgagcg tgagctgctg ctgcatcacc cgcaggccgc ccacgccgag gacgaccgcg
    18661 gtgttcaggg tcgtcaggag ggccggcacg gcgttgagga cctgggtcgc ggcggccagc
    18721 tcctggccgg agttgctcgc cctcgcgtgc tggccgctcc agcgggagaa gaacgccggc
    18781 tcgctcccgt tcgccttgag cgtctcgatg atctggagcc cgttgagcgt gacgccgagg
    18841 agcttgccct gctcctgcag gacgcgcttg gcgaggtcgc gccgccggcg ggagacggcc
    18901 gcgaggacga ggaggctcgt cgcggcgagc acggccacca cggccgtgag gacgacgtcg
    18961 aattgcgcga ggaccaggag gtagaggacg gccatgaggg cgccgaggac gttcgtcgcg
    19021 agctgtccgg agaggagctg cgcgacgcgg tcgttgatcg cgacgcgggc gccgacctcc
    19081 ccgccgaacc gctgcccgaa gtagtccatc gggagccgca gcaggtggga gaggaaccgg
    19141 ctcgaggagg tgagggagag cttcgcctgc aggcggtaga gcgtccgctg ctgcagggcc
    19201 gtcagggcca ggagcatcgc gatcgccgtc cccatgatcc agaggagcgg cgtcgtccac
    19261 gagcgatggc ccccgacgag gatctcgtcg acgaagaccc gcgtcaggct cggcaggacg
    19321 aggcccagga cggccagcgc gaaacccgtg gcggccgcga cggcgagagg cccgccgagg
    19381 ccccgggcgc gccgggccag ggagcggaac aggctcggcc cggttccgcc cggctggaaa
    19441 tcgggccccg gccggaacgt caggacgacg cccgtgtacg acgtgtcgaa gtccgcgtcg
    19501 gggacgagcc gcggcccgct cgccggatcg ttcacccacc atcccttccg cccgtgcccc
    19561 tcgacgacca ggaaatggtt gaactcccag aagacgacga acggcgccgc cagatcccgg
    19621 agctcggccg gacccatcgc gaagccgcgg gcctcgaggc cgaagtggcg cgccgcccgg
    19681 accacgttcc ccgcgttgct gccgtcccgg gagacgccgc aggcgaggcg cagcttctcg
    19741 agcggttcga agcggccgtg gtaggcgagg accatcccga gcgacgcggc gccgcactcc
    19801 gccgcctcca tctggatgac ggtcggcgtc cggcgcctcg cggggcgggc tctgcgggtg
    19861 acgtcctcgg ccggcacgct ccctcgatcg ccggcctaga ggcccagcag ctccttgagc
    19921 ttcgggacga cgagggtgat cggcgccgtc tcgcgcacca tcacctgggc cgtgcagagg
    19981 gtcccgctcg agacggagag cgtccggccc ttccgggccg accaccggaa cccgttcttc
    20041 gccgccaggt cgaggaccgg ctccgcggag acgatcgtga ccgggcccgc ggcggtcagg
    20101 acctcgacga gcgcctcgtt cgagaggagg ctcgtcagcc cgtcccgcga cgccgggtag
    20161 tcgctgacgt ccgtgaccct cgcgagcatg tagccgtact cctcgagctt gacggtcgtc
    20221 ggcgtcaggc gcacctccat tcccgggcgg atgctccgcg cctccagcgc cgggacggcg
    20281 accccgacgc gaagcgtccc cgcgtcgcgc tccagggcca ggatcgcctg acccggcgag
    20341 acgaggctcc cggggttgac gagggactcc gtgacccgcc cggcgtaggg gctcacgacc
    20401 tcggtgttcg tccggtgctc gagctcgagc tgcgcgacct cgttcctctc gtcgtcgagc
    20461 tcctgccgga tgcggaagac cttcatctcc tcctgctcgt ccaggtcccc gatctgggcg
    20521 aggagggcgc cggccgccgt caccgcctgc tcgagctcga tccgcgtggc gtcccgcgtg
    20581 tgctccagct cggcgagcgg ctgacgcgtg gtgaggccgg cgtcgacgag cttccgcgcg
    20641 ccgacgatcc gcgtctcgag ccactcgagg cgcgtccgcg ccgcgcccgc ctgctgctcc
    20701 gcctcgcgaa tccggcggcg aaggacgtcg cgctggcgag acgcctcctc ctgggcgaac
    20761 gccacggcgc gccgctcctg ctcctcgagg tgggcgagct gcgactgcat gttccggagg
    20821 cgcgccgtga ggtcggggcg cgacaggacg gcgacgacct cgccttccct cacgacggcg
    20881 ccgggcgccg cgcgcagctg ccagagcgtg ccgctcccca ccgagacgac gcgcacaagg
    20941 ccgccgccgc ggatgaagat ccccatcccg tcgacggacg tcgtgacgcg gccccagatc
    21001 ccccagatcg tcgcggcgag aaggagcgtc gagagcgcgg cgacggcgat ccacgacctc
    21061 ggaccggtga cgacgaggag ctcgtcgagc cggtcggggc tctggagctt ctcgagcgcc
    21121 tcgcggcgaa agagcccgct cacttcggac cgcccgcgcg gaccggccga gcggcccggt
    21181 ccggtcctcc ggcacgtcgt cggttcattc cgctcccccg ctccggaccg cgagatccgg
    21241 tccccgaaag gaccggggcc acccgtcagt gtagcagccg cgaacgggtg gaccccggtc
    21301 gggaagcgct ccggggatct tcgctacttc tccggcgccc ggtcctcctg cttcttccgg
    21361 atcaccatca ccttcaccgc gtcgccgtcc tcgccttcct tcgcggccca ggtcatcggc
    21421 atcgccccgg gcgcgaagac cgccccgctc tcgatcagct cctgcaggac ctgctcgacg
    21481 gccccccgcg tccggacgtc gaggctctcg aggctcttcg gcgccgcctt ctcgaggacg
    21541 tccgcggcgc tcggcctttc agtaccctcg ccgagcgtgt aggcgtacgc gcgcttcttc
    21601 acgacgaccc tcttcccgtc gtcgcccgtg aagaccatcg ccgggccgtc cccttcgccg
    21661 accctcacga cgtggacttc cttgccgccc gggaggagga cgtgaggccc ttcgccgtcc
    21721 gcgagggtct tcacggtgaa ctccttctcg ccgaccttca gcgtgtagcc gtcctctgtg
    21781 cgcgtcacga cgaccggctt gcccgactcg gaggtcagcg tcttcgtctc gcccggcttc
    21841 atcgggtcga gcttcaggac ggtcgcctcc ttgtccttgg aggtcgcgat ggtgatcgtc
    21901 gtctcttccg tcccgtcgcc cgcctgggcg gttctctcga acgggaagag cggggcgaag
    21961 agggtgatca cggcgccggc cgtgagagcg ccgagggcgg cgcgggagag gatgcgggaa
    22021 aggctcatcg ggtcctccgt ctgcttcgtc cggcatctcg ccggctttcc ccgtcagagc
    22081 gcaagcgccg ggccagccgt tcggggcttc cggccgggcg cccggctccc caaatgggga
    22141 aaccgctccc gaaagcgggc gttcccccgc gcggagggac tactcgtccg cgggggcgag
    22201 gccgaacttc tcgaggcggt agaggaaggc cttgtaggga aggtcgagga gccgcgcggc
    22261 ccgggcgcgg ttcccgcccg cgagctcgag cgcctgccgg agggcgtcct tctcgtgctc
    22321 ctcccaagcg aggcctcccg gcgggagctt ccaccccgcg tcggcggcgg gtcttcctcc
    22381 gagcggctcg gggaggtcgc ccgccgagac gcgccccccc tccgagagga ggacgagccg
    22441 ctccacgacg ttccccagct cgcggacgtt cccgggccag gagtggtcga cgagccgggt
    22501 caagagagcg gacgggaacg gctcgacgcg gatgccgtaa cgccgctccg tccgggcgac
    22561 gaagtgccgg acgaggagcg ggacgtcctc gcgcctctcg cggagcggcg ggagcgtcac
    22621 ggagacgacg ttcagcctgt agtagaggtc ctcgcggaag cgcccggagg acacctccag
    22681 cgcgaggtcg cggttcgtgg ccgcgacgat ccgcgcgtcc gtccggatct catccgcccc
    22741 gccgatccgc gtcacgacgc cttcctggag cgcccggagg agcttcggct gcatcggaag
    22801 cggcagctcg cccacctcgt cgaggaagag cgtccctccc tgggcgagct ccagcctccc
    22861 cttccgcgtc cggtcggcgc cggtgtacga acccttctcc gcgccgaaga actccgactc
    22921 gacgagcgtg tccgggatgg cggccgcgtt cacggcgacg aacggccccg cgcggcggcg
    22981 ggagagggcg tggagcgccc gcgcggcgag ctccttgccg gtgccgctct cccccgtcag
    23041 gaggacggtc gcgtcggtcc cggcgagctt ctcgacctgg cggtagagcc gctccatcga
    23101 ggggctcttc ccgacgaggt cgacgagacg gtcgcggtcc cggacctcct cggagaggcg
    23161 ccggttctcg tcggcgaggc gccgcgcgcg aagcgtccgc tcgacggcga ggaggagcgc
    23221 ggcccgctcg aacggcttcg tcaggtagtc gtcggccccg gccctcacgg cggtgacggc
    23281 gtgggcgatc gtcccgtagg ccgtcgccat caggaagccc gtctccggga agcgggcctt
    23341 cacctcggcg aggagctccg tcccgtcgcg tccggggagc ttccagtcgg agaggacgag
    23401 gtcgacgggc gaggccgcga gctcggcgag cgcggcgtcg acggagccgg tctcggcgac
    23461 ggagtacccc tcgccgcgga gaatcccggc cacgatcttt cgctggtcga tctcgtcctc
    23521 gacgaggagg acccgcgcct caggcacgcg cccccccgcc gcgatggccg accgccagga
    23581 ccgcccgcgt cccgcgcggg gcgcgggcct cgagagcgag gctcccgccg tagcgcgtcg
    23641 tggcgatccg gtgcgcgagg aagagcccca tcccggagcc gctcggcttc gtcgtcacgt
    23701 gcggcgtgaa gagccgggcg cggacctccc cggggaggcc cgacccttcg tcctccacgc
    23761 ggacgacggc gcgcccctcc tccccggcga ccgagacgac gacctcgccg ccgtcggggc
    23821 tcgcctcgac ggcgttgacg acgagagcgt ggagggcggc cttcagctcc ggcgcgacgg
    23881 ccgtcaggcg cgcggcgccg cccggcgcca ccgacacccg gacccgcccg cgcgcctcct
    23941 ggagcgccgc gagggccacc tcctcggcga gggcgcggac gtcgacctcc tcctcggcgc
    24001 cgccctccgc agcgagcgcg aggaaggagc ggatcgtccc gtcgaccccg gcgatctgcc
    24061 gccgcgcggc cgcggccatc gcggcggcgt ccgggtcgtc gggaaggcgc gcggcgagct
    24121 cgtcgaccga gaggccgacg gcgtggagcg ggttccggag cgcgtgcgcc atcccgcgcg
    24181 cgacctcgcc catctcgccg aggtgctcgc gctccttgag cgcgagcgcc tcgcgctcga
    24241 gctcctgcag gcgcgaggac atgaggttga acgaggcgat cgccgctccg acctcgcccc
    24301 cctcgcgagt ctcgacccgc gcgccgaact ccccctcgcc caccgtccgc gccgcgcggg
    24361 cgagcgcggc gagcggccgc gtcacgcggt gcgcgacgaa ggccgccgcg aggaggccga
    24421 cggcgacgat cccgagggaa ccgaggacga gccgtgtccg gaagcggccg acggcgtcct
    24481 tcacgccggc tcccgggatc gggatcttca ccttctccac cggcccttcc aggaagatga
    24541 agccggtccc ccctctcgtc gcccaggtct tcgtcgagga ggtcgtccga ttcccgtcgg
    24601 tctggaccgt cgtcacgacg aatcgcggcc cctcgccctc cttcggaacg gcctccggtc
    24661 cggccgcccg gtccttcgcg ggcgcgtccg ccgcgggccg cggaggaagg gtcacgaggt
    24721 gggcctcgtc gcggacgacg atcctccgga cgcccgcgtc gccgcccagc tcgaggagct
    24781 tcccgtcacg gcgctggagg accttcacga cgtcctttcc gaccgacgtc gcgaccgccg
    24841 tcacctcgac ccgcaggtcg cgggtgagcg cctccaccag gagccattcg gcgccgacca
    24901 tcagggcgag caggccgccc atcagggcga agagcttcac gcggagcgtc atcggggact
    24961 cgaagataca aggtccgtgc cgtcgccccc ctcccggccg gcgagtggcg acgtcccgcc
    25021 gccgaggacg accacgtacg gccgtccctt cgtctccggc aggtcgtact cgagccgcgg
    25081 atcgtccagg agcttccgcc ggacgtcggg cggcgcgtag tcgaggagat agaagacctc
    25141 tccccacgac gctccgcccg ggtcgacgac ctcccggatg agccgcgccg cctcgacgag
    25201 ctgctccggg gtccggtcgg cctcgtgctt cgtgtagcgc cccgacttgt tgttgaccgt
    25261 gaacgcgcgc gagtcagcgt cccagaggat ctcccccccg atccgtcccg gcgccccccc
    25321 gagcgccgag acgtgcccgt acgtctcgac gtaccccggc tcgcggtccg agccgtcctc
    25381 cgggcgatgg aagccgttcg ggtaggtgcg accgagcggg tgcggcacct cgcgcaggat
    25441 cgcgacccgt ccggcgacgt cgatgaccca cttgtacgtc ccgtctcccg gctcgaggag
    25501 cgcggggaag tcccgcgtcg ccacctggag gtacgggttc aggaggaccc ggccgctccg
    25561 gtccgcctcg tactgcttga cgtaggcggg cggaacggca gccccgtact tcgcgtcgag
    25621 cgggtcggcg ggcggcgcgg cgagcgccga cgtcgccgcg aggaggagcg ccttcgccgt
    25681 ccaggacgcg taccggggca tccgggcctc ctgcgcgcgg gagagggaga ggccgcgcgc
    25741 gacgcgagtc tagcggtccc gccctccgcg tccggccgat ccaggccgat tcggcgaaag
    25801 cgcccccggt cgggcggacc gacgcccgat ccctcccggc gcgggtttcc gcactcggcc
    25861 gcgcgggaac ccgcacaaca cagcctcccg tcgtccggac gcagaggtca agcctttgga
    25921 gcagaatcgt ttcgagacga acggcgtccg cggcacgacg cttgcgccct gtccgtcgtg
    25981 gagcccgcgc gggctccacg atctcgagct tcaagacgca agggaggagc cctcacatgg
    26041 cccaggagat cgaacggaag ttcctcgtcg ccggtccctt caaggagcag gcgacccgac
    26101 agacccgaat cgtgcagggg tatctctcct cggtcccgga gcggaccgtg cgggtgagga
    26161 tcaagggcga caagggcttc ctgaccgtga aggggatcgg gaacgcttcc ggcgcgtccc
    26221 ggtacgaatg ggagagggag gtgccggtgg ccgaggtcga gcagctgctc gcgatctgcg
    26281 agccgggcgt catcgacaag gtccgctggc tcgtcccggc gggcgagcac acgttcgagg
    26341 tggacgagtt ccacggcgag aacgaggggc tcgtcgtcgc cgaggtcgag ctcggcgccg
    26401 aggacgaggc gtacgcccgc ccggcctggc tggggacgga ggtcaccggc gacccgcgtt
    26461 acttcaacag catgctcatg aaaaacccct tcacgcgctg ggagcgcccc gcggacggcg
    26521 ccgagggcgc gtgagcgggc cggaccggcc ggcggccgcg gcggacggga ccccgcgggc
    26581 cccgacggcg gtcgatccgc tcgacatgcg gaactacggc ctcgagaagc tgcaggagat
    26641 caagcccggc ccgttcatgg cgcgagcgaa gctggccggc ttccccctcg ggatcctcgc
    26701 gttcctcttc ttccacttcc gctggtgcgg cccgatcggc ttcttcgagg cggcgaccgg
    26761 cgtcgcgaac gtcggccacc tctacaccgt caccgggatc ttcttcttct cgctcgtcct
    26821 ctggatgacg gaggcgattc cgagctatct gacgtcgttc atcgtcatcg tctcgaccgt
    26881 cctcttcggc atcctgaaga tgcggccgac cttcgcctac ctcggcgagc cggtcatgat
    26941 cctgaacatc gcgagcttca tcctggcgag cgccctcgtg gcgagcgggc tcgcgaagag
    27001 gatcgcgctc ctcctcgtca cacggtgggg gaaccacatc acggggatct tctgggcctt
    27061 catcgccctg aacctcctcc tcggcgcgtt catcagcgcg acgtcggcga agaccgcgat
    27121 cctcctgccc ctcttcatgg tcatcgcggc gatgtacggc gccgtcggcg gggtctcgcg
    27181 caacaacgtc gggcggaacc tcgtcctcca gaacctcctc gcgaacaacg tctcggcgag
    27241 cgccttcatc accggctcgg cggcgaacct cctcgccgcg cagctcctcg agaacgccgg
    27301 ccacaaggtc atgtactccg agtggctcat ggcgctcctg ccgctcgccg tcatccagtg
    27361 cgccatcgcg tggttcaccg ggacgaaggt catcttcccg gtccggcacg aggacgcgca
    27421 gccgaaggtc gacggaggca tggaccgcct ccgggccgag ctcgcggcgc tcggccgccc
    27481 gagcctccgc gagctccgcg ccgccgtggt cttcgtggcc gtcctcgcgc tctggtcgac
    27541 ggagccgctg cacgggatcc gggccgaggt cgtcgcgatg tgcggggccg tcctcgtcct
    27601 cctcccgccg ttctccgggc tcccccgctt cggcgtgctg acctggaacg aggccgacat
    27661 cccctggcac atgctgatgt tcagctgggg cgcctacgtc ctcggcggca tcatggagtc
    27721 gacgcacatc gtcgacctgg ccgtcgaggc gatgttcggc catctcggga tcaccgcggc
    27781 gacgccgaag gtggcggtct tcctcgtcct cgcctccctc ttcgccttca cgacgctcat
    27841 caacgagagc aagacggccc ggacgatcgt cctcttcccg gtcatgatcg cgacggcgac
    27901 gaaattcggg tgggacgtcg tcggcttctg cctcccgatg gcgttcctga tcaaccaggt
    27961 ctacgtcctc tacttcaact ccaagccggc gaacatcagc tacctctcga acatgtactc
    28021 gaccggcgag tcgttcaagt tcgggatcac gcagctcgtc ctcatcctcg tcgtcctcac
    28081 gctctggacc cagtacgtca tgccgctcat gggtttccag agccggctct ggtgagccgg
    28141 tagctctccc gtcgatctcg agcccgctcc ccggatcgcg ccggggggcg ggctcttccg
    28201 tctcccgtca gggggcgacg acgggaacga ggaagacgtt cgcgaggacg aagagcccga
    28261 cgatggagag cagggccgcg accgccggcg cgaggaccca ccccgcgacg atccccccga
    28321 cggtccgcca cttcacgggg ctcctcctca cgagggagat gccgaggatc gccccgacga
    28381 ccgcctgcga gctcgagacg gggacgagcg gcggggccgg caggccgtgc gcccgcagga
    28441 gctgcgacag gctcggggag gcgaagaccc agagcaccgt cgccgtcgcg gcgacgacga
    28501 tcagggccgc gagcggcgag agcgccgtca ggcccgcgcc gagccgcttc atgacgcggt
    28561 gggagtaggt cgcgatgccg gtcgcgatcg cgaggccgcc ggcgaggaag aggatctcga
    28621 cgctcgaggc gcggagcggg ccgagcgcga cctcctccgg gaacggcgag acgcggagga
    28681 agacccccat cacgttcgcg atgttgttct gcccgaggct gtaggcgccg aaagccccga
    28741 cggcgaggag cccggcgcgc gtccaggcat cgacctcgag gaggtggatc cgcacgtgcg
    28801 gcatcgccgc ccgcgccgcg acgaagagga gcgccgcgag cgcgcccgaa aggagcgggc
    28861 agaggagcca cgaggagacg atccgcccga ggaccgccgg gtccgtcgcg cggcccgtgt
    28921 agaggttcca tccgacgatc gcgccgacga tcgcctgcgt cgtcgagacc ggcaggccgc
    28981 tccgcttcag gaggagcagg acggcgatcc cggccgcgag ggcgaccgtg aaggcccccg
    29041 ccggagcgtc gatcgccccg agccgcccga gcgtcccggc cgctccggag acgcccgcca
    29101 ccgagccgag gacgacgaag aggccggcga cgagggccgc cgtccggaag cggaccatct
    29161 tcgtggcgac ggccgtcccg aagacgttcg cggcgtcgtt cgcgccgagc gaccagccga
    29221 ggaagagacc gctcgagagg aagagccagc tcaacgcgcg gggacgtccg gaggcttcag
    29281 gaggccgagc cggccacggc gctcgccccg gcggccggtc gcggcatggc gctcacgggg
    29341 agctcgaaga cgttctgaac gacgaagaga cccacgagcg cgaacaggag cgcggccgcc
    29401 ggcgcggcga cccacccgat cgcgatctgc ccgagaggct tgagccgaat gccgctcttc
    29461 ctcacgaggg agatcccgac gatcgcgccg acgacggcct gggtcgccga gaccgggacg
    29521 agcggccagc cggggagccc cgccgcccgg aggaggcgct ggagcgcggg agagccgaag
    29581 aggaagagga ccagggccgc cgcgaggacg acgatgagcc cggcgagcgg cgagagcgac
    29641 gtcagccccg atccgagccg gaccatgacc cggcccgagt aggtcgcgat ccccacggcg
    29701 atcgcgatac ctccgagcag gaagagcagc tcgacgctcg tgaagcggaa cggcccgatc
    29761 gccgtctccg cggggaaggg cgagacccgg acgaagaccc ccatgacgtt cgcgatgtcg
    29821 ttcagcccga ggctgaaggc tccgaacgcc ccggcgacga ggagagccgt gcgggtccag
    29881 gcgtccacct cgaggaggtg gacccgtcgc cgctggagga ctcgacggga gacgacgaag
    29941 agtcccgccg cgatgagacc cgccaggacg gggctgagga gccaagagga gaggagctgc
    30001 gtcagcgtcc tcaggtccgt cgcctttccg ttgaagaggt tccagccgag gatcgcgccg
    30061 acgatcgcct gggtgatcga gacggggagc cccgaccgcg tcaggaggag gaccacggcc
    30121 gccgccgccg cgagggcgac gacgaacgcc cccgcggggg cgtcgatggc gccgaggcga
    30181 ccgagcgtct tcgaggtccc gtgggcgccg gcccacgccc cgatcacgac gaagacaccc
    30241 ccgaccaggg ccgccgtccg gaacttcagc atccgggtcg cgacggcggt cccgaagagg
    30301 ttcgccgcgt cgttggcgcc gagcgaccag ccgaggaaga ggccgctcgt caggaagagc
    30361 cacgacatcc cgcccgcctc ccgttcagat cgtccgcttg atcgtgtaga tcacgagccg
    30421 gtcggcgaca ccctccgccc ggtccgagag cgtctcgacg tggaggcaga agtagcggag
    30481 gtggatcttc cgcgcgagct cgaggtccat cgcgaagatc ttccgcttca gcccgtccgc
    30541 gagccggtcg gcctccttct cgaagaagta gaccttgtga atgtggctcc tgaccgccgt
    30601 gatctcccgg aagaaggccc ggcaggagtc gacgaggtgc tgcgtcgcct ggacacaggc
    30661 ccgggccagc gcgaggtagt ccggggcgag ctcctccggg atctccggcc gctccacctc
    30721 gaactgcccg agggtctgct tggcctggtc gatcacgttg tcgaggttct cgaggaggcc
    30781 gaggacgtcg ccccggtggt ccgggatgag cgactcggca tacagccgcg tctcgacctc
    30841 gcgccgcagc gcgtccgcct tcccctcgaa gacgccgatc gccttccggc gctcctcgaa
    30901 gctcggctcg tcgtgtcgga ggtagtcgtc gacccccttc tcgaagacga gacacccctc
    30961 gctcacggcg tcgaggaact ggtcgatctg cgcctccagg gcgcgtgtcc ggcgaaagag
    31021 gagagtcacg ctgcacctcc ggcggggacg gagacgagga gaggtttcgc tccggcaagg
    31081 accgtctggg cgggtcggga agccggtcca cgacggtccc cctcgacgtc aggctcgtcg
    31141 cccttctcgc ggtccgatcc tagcgccgga ccggtcagga ccgcggcgag gagcaggagg
    31201 agggccacga cgacgaggag gagcggcggg cgcccggact ccggcgtcca tttcacgagg
    31261 gccggcctct ccgttcggcc cgccgggacg gagcggcgca cgtcagcccc gacgcccgcg
    31321 agcggggatc gccgcccgac acccagagca ccgatccggc gcccgccgtt ctctctggct
    31381 taacatgtcg gcctccgacg cccctcgtcg aaagaaaggg cgggcgcgga cgacccgcgc
    31441 ccgccggaag gaggaggggc ggaggggcga cgaaccccct ccaccggaac gcgccgatca
    31501 gccgaccggc agaccggcga accgcttgag cgccttgatg tagttcgtgt tcgggaactt
    31561 gtcgaggccg agcttgcgca cggtcgcgat gacgagcgcg gggtcctcgt gctccacggc
    31621 ggccgtccgg atcggcgtgc cgtcgaacgt caggttcgcc agctcgacga tgcagccgtc
    31681 gacggtgagt ccgaagcgct ccttgccgac ggcgatcgcc cgcagcttcc cgtgagcgcc
    31741 gacgacctcg gcgaggaact cgtcgagcga atagcccgcg cgggccagct ccgggagcgc
    31801 gacgccccac gccgcggcga cctgagagag gacctccttc gtcaggggga agcccgactt
    31861 caggaccggg ttccactgct cgagcgtgtc gccgttgacg ttctcgagct tcttgatgtc
    31921 catcaggccg tcgcggatct tggtgttgtt cgggctctcg gccgagacga tgtacgtctc
    31981 ggcgctcgag cgcgccttcg cttcgccgtg ggaccgtatg ttcgactcgg cctcgccgaa
    32041 gctctcgccg aaggtcctgt actcccagcg ggggatgatg acgggggccg cgttgctcat
    32101 gtcgatactc ctggcacggg cggcgggaag gcccgaccgc gggttctgtg acggggcgcc
    32161 gggaggcgct ccttcgtgaa gcgcctcccg gcggccggcc gggctaccta gaaggcgtac
    32221 tggacgcgga actggaggac gctgaagtcg tcgccggcga tggcgccgta gagcttcttc
    32281 ggcttggcgt actcgtcgtt gtcgacgatc gaccagtcca gcatcaggcg gatgttgaag
    32341 ttcgggtagt agttcagccc gagggtgtag ttctcggcgc tgccgccctt gatcgggtcg
    32401 acggcggtga ggtcgttgag gtccatcgtg ctgtagcggg cggcgatctc ccaggccccg
    32461 cccttcccgg gagacacctt gtcgacgaga ccctcgtcgg ccgcgtactt ccgcttgccg
    32521 ccgaggacgt acgagacctg ggcgtaccag ccgtcgaagt cgtggtcgga gaggtcgacg
    32581 gcgcccgaga gacggtcgac gctcgtcttg ttgtactcgg cctgccaggc gaaggcgtcc
    32641 cagacgcccg cgagctcgag cccgtactgc cggacctggt cgacgtcctc gaggtcgccc
    32701 gtgttcaggt acttcgcctt cgcgatcttc gagacctccg gccggctcga gaactccacg
    32761 acgtaggccg ccggcggccg ggtgctgccg tcgccgaccg cggcgtccgg ctgccagccc
    32821 gtcgccgcca cgccgacgtg gatcgcgcgg gtctccgcgg gcatgaccgg cgcccacgtc
    32881 ccgcgggccg cccagcccca gccctggtcg aggccgctca cgtcctcgcc gatcccctgg
    32941 gtgaagaggg tcgccgccgc ggagaagcgg tccgcgccgt agaagtagcc gacgccgagc
    33001 cgccgccccg gggcgaacgc gtcgacgtag gcccgctcga cgaaccagat gtccttcgac
    33061 gagacgagct ggtcgtagcc ataggggatc ttgaactggc cgagacggat cgcgctcttg
    33121 tcgatcccct cgtagccgac gtaggcgtcc ttgatgtcga ccgcgttgtc ggcgaagtcg
    33181 acgtcgaact cggcggtcca gtccttcgcg accgtcgcct tgaagccgat gcggccgcgg
    33241 cggaggccga gggcgttccc gttccggttc tcgtccccgg agaagaacac gccgtcgagc
    33301 tgcgcacggc cggcgagccg gaacttgtac gcgccgtccg cgctcgccca ctcgagcttg
    33361 cccttgtcgc tcacggagcg gagcgtcgtc cccttcgcgg aattcgcctt cgcctcggcg
    33421 gccgagcgct ccttgtcctt cttgacgagg ctctcgaccc tcttctcgag gtcggcgatc
    33481 ctctccgcgt ccgactgcgc cagggccgcg cccggggccg cgaggagggc caccaggacc
    33541 gggatcccga actgtctctt cattgcgatc tcctgcgttt cttcacgccg ctgccgtggt
    33601 cattcgccga tgttccgggc gcccggccag cggtaggtgt aggggttccc cttctcgtcg
    33661 agggggcgct tgtcggcttc cgtcacgacc gacgtctcga agtcgaggat cgcgtcgccg
    33721 atcgtcttga tcgcgacggc ccgccccttg aagcccggcg acgtgagctg gagcttcccg
    33781 tccttgccgt aacccttgta gtgcgtgaac caggtctgga ggatcgtcgt cacgccgggg
    33841 aagcgcttgt cgaggtcggc gaggctgctc accttcgcga acggccccga ctgcatgacg
    33901 aggaggacct tgtcgtcgat ctcgccgttg tcgaggagcg tcaggatccc gatggggcgt
    33961 gccttgacga cggagccgat cgggaccgtc gggccgagga cgcagacgtc gagcgggtcg
    34021 ccgtcgcccc ccttgctctt cgggaggagg gaccggggaa tcaggccgta gttgcacggg
    34081 tagccgaggt actggacgaa gcggggcttg ccgttcttct gctcgagctc gagcatcccc
    34141 gtgtccttcg tcgtctcgta cttggcggtg gtccccgccg ggatctcgat gacggcgttg
    34201 atcgtgccgt cgaggttgat cgggtcgaag tccgtcagga agttcgattc gcccctgagg
    34261 accgacacgt gcggtgtgac gccctgggcc gcgacggccg gggcgagcgc ggcggccacg
    34321 aggacgagcg ccagcgagag cgcggcgaac ggggtcttct tcatggtcta tcggctcctt
    34381 gtcctgtctc tgctcgtcgc cgggcctcag gacggatcgt cgttgtgcgc cgccttggcc
    34441 ttcttcttct ccggggcggc gggggcggcg gctttcggcg cctcgacggc ggcggcggcc
    34501 gcgaccgggg cggcggccac ggcgatcggg gcgtcggtgc tcagagtcga gggggcaccc
    34561 gccaggacgg cgacgtgggg gcgcttcttc ttcttctcgt cgtcgtaccg gaggtccggg
    34621 ctcttcatca gctgctcacg gacgtcggcc ggcgcgtact cgaggaggta gagggcgggc
    34681 ccccaggcga cgcccgccgt gtcgacgacc tgccggatca gcctcgcggc gttcgccagc
    34741 tgctgcggcg tccggtcctt gttgtgcttg ctgtagcggc ccgacttgtt gttcaccgtg
    34801 taggtgcccg tctccttgtc gtagaggatc tcgccgctga tccggccggg gccgccggcg
    34861 agggaggaga cgtggccgta gttctcgcgg gtcccgggct tcttctccga ctggtcttcc
    34921 ggacggaaga atccgttcgg gtagacgcgg ccgagcggat gggccgcctc ggggatgatc
    34981 gtgacccggc cctcggcgtc gatgacgtag ttgtacgggg ccatcttcgc gtcgaggatg
    35041 gcggggaact ccttcgtcgc gacctggagg taggggctga tgaggacctt gccgtcccgg
    35101 tcggcctcgt agtgcttgat gtacgccggc tgagaggcga cgccgtagag ctcgtcgagc
    35161 ggatcgacga gcgggccggc cgccacggcc gcggcgccga ggggccggtt catcagcttg
    35221 cggaccttgg agccgcccca cttctcgctc tcgggcatgg cgatcgccgc gagctcgccg
    35281 tggaggacct tcgtcaggaa ccgctcgccg gcctcgtgga ccttcgcgat gtcgtagaag
    35341 tagaggtcgg agtagccgta ggagaagtcg tagaggtacg gggcgccgcc ttccttgtcg
    35401 gagaaggcct ccatggagac gccgcacggc tccgtccccg ggaggacgac cgcgcccggc
    35461 ttcacgcggc tcgagtacgc cttcttcccg acgagggcgg tgtcggccgg gaggccgatc
    35521 gagaggagct cggggacgaa ccggccgaag tcggcgagcg tcatcttccc gagggcctcg
    35581 accgggaccg tgaacgtcgc gcccttctcg atgtactcgc ggaggctccc gccggggccg
    35641 atgccgacgt tcccctcggc ttcgatcttg accttctcgc cgcccgtgac ggcgagcggg
    35701 gcggccatcg cgaggcgggc gtcctccttg acgcacttca cgttgacggc gacgtccctc
    35761 gcgggctttc cgtcggcata cttgttcgtg acgcggatct ggtagccggc cttccagagg
    35821 agctggtcgg gggtgtcgag gtactccttc gtcgcgagct cgaccttgaa ggggttcttc
    35881 tccttctcgg tcacctcgaa gccgttcttc tccgcggccg cccggactct cttccagatg
    35941 tccttgaagg cctccgccgg cttcgccttc atcttcgccg ggtcgatgag gaacttgtac
    36001 tcgcggcctc cgacgtcgtc gagcaccggg gcccggtact tctggatcgt ctggacgtag
    36061 agcggcccgg gttcgtcggc ggcgaccgca acgcccgcgc agccgatgac gagtgccagg
    36121 gccagaaggc ccgatacggc tctcttgtgc attcgatacc ctcctcgcga ctctctcccg
    36181 gcctcccgcg gccccgggcg attcccggct cccgggaggc atccggtctc ccggtccttc
    36241 tgcaaccgcc gggccatccg cccggccgcg ccgggaccgc gtagctctgc cctccaggac
    36301 gctgccgcgc gggccggaac gcgcgccgcg tgcgggatcc cgcacgcggc gtgcggagaa
    36361 ccgctcactc cctcgcgccg ccgagtccga ggctcttcat ccggtcgtag agcgtccggc
    36421 gggccatccc gaggatcgcg gcggcctccg tgacgttccc ccccgtcgtc gcgaggacgc
    36481 gctcgacgtg gagacgctcc atctccttca tcgtcaggag ccgatcgtgg tcgcgcggcg
    36541 gcggcgccgc gacggcgtcg agcccgaggc cgaccgcgtc gagcgtctcg cccttcacga
    36601 ggatcatcga gcgctcgatg acgttcgcga gctcgcggat gttcccgggc cacgagtacc
    36661 gccggagggc ggcctcggcg tcctccgtca ggcgcgccga cggccgtccc atccgaccgc
    36721 agacgcccgc gaggatctcg cgcgcgagga cgaggatgtc ctcgggccgc tcgcgaagcg
    36781 gcgggatccg gagagtgagg gtcgagatcc ggtagtagag gtcgtcacgg aacctcttct
    36841 cgcgcaccat cgcctcgagg tcgaggttcg tgctcgcgac gagccggatg tcgacgtgcc
    36901 ggtcgcggac gtcgccgagg cgacggaacg tcttctcctc gagcgccttc aggagcttcg
    36961 gctggatcgc cgggtccatg tcgccgatct cgtcgaggaa gacgatcccg cggtgcccca
    37021 cctcgaggag cccgggcttc gggttcaccg cgccggtgaa ggcgccgcgc tcgtggccga
    37081 agagctccga ctcgaggagc tcgcgcgtca ggcccgcgca gttgaggttc acgaacgcct
    37141 cttcgcggcg cgggccgttc gcgtggatcc accgggcgag gacccccttg cccgagccgg
    37201 tctcgccgag gatgaggaca gggctgtccc actccagcat caggcgcgcc cgctcgtcga
    37261 gcctcctcac ctgcgggctc gtcccgacga acgggtcgag gaggccgggc tgcgtcctcg
    37321 cgacgatctc ctgccgcttt cggagccgct gctgctggag gagacgcttc aggaggacga
    37381 ggagcgcctt cgactcgacc ggcttggtga ggaactgctc ggcgccgtcc ttgatggcgc
    37441 ggaccgcgag gtcgatcgag ccgtgggccg tgaggatgac gaacggcgtc tcctcggaga
    37501 gcttcttgac ctgcgggagg atgtcgagcg aggtgccgtc ggggaggctg tagtcggcga
    37561 cgacgacgtc gtgccccacg agccggaagc gctccatcgc cctctggcac gtctccgcct
    37621 cgtcgacgtc gaggccgttc gcgcggagga aggcggccat cccgtggcgg accgcggggt
    37681 cgtcctcgat caggaggacc ttcggctcag gcacgcgcgc ctcccgccga ctgtagcggc
    37741 tggtgcgggg cgggcaggcg gaccgtgact cgggccccgc cctccgggcg gttcgccgcc
    37801 tcgacgccgc cgccgtgctc ctcgacgacc ctcttgacga tggagagccc gagcccggtc
    37861 ccccccttcc ttcgggtgaa gaacggctcg aagccgcgtg agagggcctc cgcgtcgaag
    37921 ccggcgccgg agtcctccac gtcgaacgtc caccagtaga agccgtccgc gaacgtcctg
    37981 cggccggcga tgcgcaccgt cgagccctcc ggcgagtgga ggagcgcgtt ctcgagcagg
    38041 ttccggaaga cctggtgcat ccgtcgtggg ttgagcggga ggacgagcgt cggctcctcg
    38101 aggtcgacgg cgacgtcgca cgagcggtcg gcggcgagct tgtcgcacgc ccgcacggcg
    38161 ttctcgacga ggctcctcac gaggtggggc tccgggttca gcgtccgagg ctcaccgtag
    38221 tcgcgaaggt cgttcacgag gctcgcgatc cgctccgcgc tctcccggag cgccgcgacg
    38281 aaggagacgt agcgcgggtc ctcgccgaac gtcgcctcga gcgcgtcgag gttgatcagg
    38341 atgccgtaga cggggttgcg gatctcgtgg accacgccga ccacgagcgc gcgcagcgcc
    38401 tcctcggcgc gtcgcctctc cgtgatgtcg gtgcacatgc cggccacccg ggccggacgg
    38461 tcgccgtccc aggcgacgac cttccccgcg tcgaagaccc agatccactc gccggtccgc
    38521 gtcctcaggc ggtactccgc ctcgaagcgc gggacccgcc cctccaggtg agcgctcatc
    38581 gccgcctcga tctgcctccg gtcgtcggag tggatcagcc ggagcccggc gtcgaacgtg
    38641 tcctcgacgc cgccgggcgt caggccgatc atcccgaacc agcgggggct cctgtagacc
    38701 ttcccggtcg agaggtccca gtcccacagc ccctgctgcg tcccctcgag cgcgtggttc
    38761 agccgctcct cgctcgcccg gagctccgcg agcgcttcct tgagcgccgt cacgtcgcgc
    38821 gagatgccgg ccacacggtg gatgcgcccc cacttgttcc ggaccggcgt aaggacggtc
    38881 tggaacgcgc gcgtctttcc gtcgacgacg tgctcctcct cgaaggggag cgggagcccc
    38941 tgctcgagga cgcggctgag gtaggggacg aaccggccga aggccggctc gccgaggcac
    39001 tcccggaccg ggcgcccggc gaagccgggg ctgaggaacc cggccccggc gttgagcggc
    39061 gggttcaccg tctcgaagcc gagcgcaccg tcttcgccga ccccgacgac gtagatccag
    39121 tcgaaggcgt tcgggacggc cgtctcgagc ggcgcctcgc cgccctccga agggaacccc
    39181 gacgccgcgg ccgcctgcgg cgcggccaga gcgtccctcc tcgcgcgagc cctctccagc
    39241 gccgccgcga agcccgcgac gcccgggaac gcctccagga agccggcccg aagcgcctcg
    39301 gcgagaaggc ccgaccggct cctcggcagg tgcgccaggg cgggcacccg agggtgagcc
    39361 gcacggaacg tccggacctt ctccgcgtgc gtgcggcacg cgtcggctcc ggcgacgagc
    39421 gtgtcgtccg gtccgcaggc caccgcctcg agctcggact cggtccaccc gccgacgtcg
    39481 acgacgagag gggccgaggt ccaggaccgc gccagggctt gcgccgtccg gctgctgagg
    39541 cccatcgtga agatccgcca cccgcttcgc tgtcctaccg tctcggtcat cggtctcctc
    39601 tgtcggtgcc tcccggtcgt tcccggtctc accccgtcgc ccctcgcctg ccgcaaggag
    39661 cggaccagcg gcacgacggc ccggtccgcg ggtcgaccgt ccatcgtagc gccggcccga
    39721 gcccggggtc ggagatcacg cgcggacctc cgtcgtctcg gcgccccgcc cggcctgccg
    39781 ggggctaaga tgccggctgc gggaatcccg atgacagacc gagccgctca cccgcgcggg
    39841 gcgacgtcgt accttcgggc cgtcctcgcc gccctcgtcg tcgcgtccgc cggttgcggc
    39901 ggcggtgaga cgttgccggc cccgctcccc tccggcaccc cgaggccgct cggcggggcg
    39961 agcgctctcg agctcttccc ggcgctctcg ccctcgggcg acgagctcgc ctcgtcggtg
    40021 atgacgaacg gcaagctcga gatccacatc cggtcgctct ccggcgggcc gggccgcccc
    40081 ctcacccgtg acggccggaa caacgtccag cccgcctggt cccccgacgg gcggctcatc
    40141 gcgttccact cgaaggaccg cggcgggatc tggatcgttc cggcctccgg aggctcacca
    40201 cgcctcctcg ccagcttcgg ctcccagccc tccttctccc ctgacgggcg gcgcgtcgcg
    40261 ttccagtcga cgccgctcac ggacgtcgcg gcgacggccg cgccggggct cgctccgtcg
    40321 acgatctggg tcgtcgacgc ggccggcggg gcggccctcg ccgtgacgaa cccggggagc
    40381 ccgatcggcg gccatggcgc gcccgcctgg tcgcccgacg gcgaccgcgt ctacttcgtc
    40441 acgtccgagc cgcgcctcac gcaggcggag atctggtcgg tgaagcccga cggcagcgac
    40501 ctcgtacggc tccacgccgg cacccggatc tacggagtct gcgtcccgcg cggagcgaag
    40561 gccctctggt tcgcgggcca ggggaaggcg tcgctctacg ccctccaccg gatctccctc
    40621 gacgagaggg ggcgcacctc cgtcggcccg gccgaggcga tgacgccgcc gggctcggcc
    40681 gtcccgcaca gcccgagctt ctccgcggac ggctcccggc tggcctggag cgagctgacg
    40741 acgaccgcgg ccatctggag ccttccgctc gaccccgcga cctcgctcgc cgccggaccg
    40801 cccgcccccc tcaccggcgg cgacggccgc gcgacctggc ccgtcttctc ccccgacggg
    40861 agcaccgtcg cgttcggctg ggtcccgacg ggcgggacga acgacgtctg gctgatggag
    40921 ccggacggga accaccagcg accggtggtc gagggcccgt cgatcgacta cgcgagcgac
    40981 ttctttccgg acggccgccg gctcctcttc ctctcgaacc gggagggagg aacgttcgct
    41041 ccgtgggagc tcgagatcgc cacgcgcacg ttccggcgca tcccggccga gctgcacgac
    41101 gccatcgctc tgcgcctctc gcccgacggg aagcggatcg cctaccactc gaagcgggac
    41161 agcatcacga tcaacgcctg ggtcgtcgac ctcgcgaccc ggacctcgca gcagatgacg
    41221 ttcgaccggg agttcgtcgg ctatccgtgc tggtcgccgg acggacgcga gctcgccctc
    41281 gaggtccggc ggggagacga catcttcatc ggcgtcatcc cctcggcggg agggaccccg
    41341 gagctcctca cccagaagcg ggggctctcc tgggcgttca gctactcgcc ggacggacgg
    41401 cggatcgcgt tcgcgggcta tcgcgacgac acctggaacc tctggtggat cgaccggacg
    41461 acgcgcgagg agcggcgcct caccgagaac ctgcggctcg acgtctacat ccgctacccc
    41521 gcctggtcgc cgaagggcga ccggatcctc tacgagcagg ccgagacgcg gggagaggtc
    41581 ttcctcctcg acggccgcgc gccgtccgcg gccgctcgct gaagccgccg cggggaaggc
    41641 cttgaagctc gagtcgttcc ggcgccggtt cttcgagctc ctcccccgct acgaatggga
    41701 ggggacgctc gggcagggag ggatcggggt ggtcttccgg gcgctcgatc gcgagctcgg
    41761 ggagagcgtc gcgatcaagg tcctctcccc gaacctggcg cgcgacgaga acgcgcttct
    41821 cgcccggttc aagcgcgagg tcgcgctcaa ccggaagatc aagcacccga acgtcgcccg
    41881 gatgttcgac tacggcatcg ccggcgacta cccgttcatc acgatggagc tgatcgaggg
    41941 ggaagacctc tggacgatga tcgggaaccg gcagcgtttc cccgcggcgg aagcggtccc
    42001 cgtcgtccga cagatcgctc gcggcgtggc cgcgatccac cgtctcggga tcctccaccg
    42061 cgacctcaag tcgcagaacg tcctggtcga ccggcacggt gccgccgtca tcctcgactt
    42121 cggcctcgcg cgcggctcgt tcgacccggg cctcacgctc gcgtcggtcc tcctcgggac
    42181 tcccgagtac atgtcccccg agcaggcgct cggcgacccc ctcgacgagc gggccgacct
    42241 ctactcgatc ggagtcatcg cctgggagat gctctccggc cagctcccgt tcgtcggcga
    42301 ctcccccgtc gcgacggcga tgaagcacgt ccacgaaccg ctgccggacc tgcgggagct
    42361 cgtcccgggt cttccggcgg acctcttcgg cgtcctcctg cgcgtcctcg cgaagaagcg
    42421 ggaggaccgc ttccgatcgg cccaggagct ggacgacgcc ctcgccctct gcgacaccgg
    42481 tccggcctcg ccgctccccg gggaacgcct cctgccggag gcggaggcgt tccttcgcga
    42541 ggtggaggtc gccatcgact ccatcatcct gccccgcgag gcgcggccct cctcgcgctc
    42601 cgacgcaccg aagacgctcg tcacggcgcg cccccgacgc cgacagccgg tcgtcctcgt
    42661 cgtcgaggag gacgtacgcg gcctcctgaa gctcgcgacg acgctctgca tgaccggctg
    42721 tcggacgctc gaggtccgca gcgccgagga ggccctcgag acggctctct cccgccaggt
    42781 cgacctcgtc ctgatgagca cgcgccttcc cggcatggac ggtttcgacg tggcccggat
    42841 cctgaagtcg attccgcgcg ccgcgcacat ccccgtcctc ctcctggctc ccggcccgga
    42901 ccgcgccaag cacgcgttcg cccttcagtc cggcgccgcg gatctcctgc ccgagccggt
    42961 cccgcaggcg ctcctcgagg agcggacctg ggacctcctc cgccgccgcg gcttctcccg
    43021 tccgagcgag agcggcccgg gccgcgagaa gggccccacc tcccgtcccg cgtcacggcc
    43081 cggctcccgt ccctctccgc gctcctcctc ccgctcgcgc gagcgctccc gaagccggag
    43141 ctgagggggg agccccgtat catccgcgcc gtccggagga cctgatgagc gacgccccgc
    43201 ggatcctcgc cctggccggc agcctgcgcg ccggctccta caaccggcgg attctggccc
    43261 tcgcggccga cggcgcccgt gccgcgggag ccgacgtgac gcggatcgac ctccgcgact
    43321 tcccgctgcc cgtctacgac gccgacctgg agtcggcttc gggtctccct tcggccgcgc
    43381 tcgcgctgaa ggagcgcttc cgcgcggccg aggggctgct cttcgcgagc cccgagtaca
    43441 actcggcgat cagcgggacg ctgaagaacg cattcgactg ggtctcgcgg cccgctccgg
    43501 gcgagaagcc gctcgcgtgc ttcaagggga aggtcgcggc cctctgcgcc gcctcgacgg
    43561 gcgcgctcgg cggaatccgc ggtctcgcct ccgtccgtct catcctcggg aacctgggcg
    43621 tcgtcctcct ccccgaccag gtcgccctcc cgaaggccgc ggaggcgttc gacgacgacg
    43681 gcgacgtcgt cgacccggcc cgcaggaagt ccctcatgga gctcggcgcc gccctcgcgc
    43741 gcgcgacccg cgccctcagg agtccgtcgt gaccgacact cccgccttcc aggacctcta
    43801 ccccgaccac tgggcccact gctacggctg cggccggctc aacgagcacg gcctgaagat
    43861 ccgcagcttc tgggacggcg acgagaccgt cgcgacgatc cacccgcgtc cgcaccatac
    43921 ctcggtcccc ggcttcgtct acggcggctt catcgcttcg gtcatcgact gccactcgac
    43981 cggcagcgcc gcggcggccg cgcaccgggc agccggaggc gacccggcga tcggccccta
    44041 cccgcgcttc ctgaccggga ctctgaaggt cgagtacctg aagccgaccc cgatcgacgc
    44101 gccggtggag ctccgctcca cgatcgtcga ggtgaaggag cgcaaggtcg tcctcaccac
    44161 gacgctcacc tcgaagggga ccctctgcgc acgcggcgag gcgattctcg tgaaggtccc
    44221 ggagacctgg gagccgagag cctgagtccc cgcgccgccg tcaactttcc ggcgccgcgc
    44281 acgtatttcc gggaagacgg acccggtgag aggtttgccc cgcctccggc gcggccccac
    44341 cacacgacag gaggatccat gaatcgagcc cgacggaccg cggccctcgc agccgcgctc
    44401 gcccttctcg ctgtcccggc cgtcgcccag ccgacggcct accaggggca cggcgccgac
    44461 agcgtcccgc ccgaggtcct cgcgaagtac gtcgcccccc cgctcccgtc ggagctctcc
    44521 cggaagatcc agaacctgat ggacgtccgc gccccgagcg gcggccggct ctcgcccgac
    44581 ggcgcgcgcc tctacttcgg ctgggccgtg accgggacgg cccaggtctg gcgcctcgac
    44641 ggcccgaaca ccttcccgat ccagatgacc gggggcgagg accgcacgtc gctcgccgac
    44701 gtcacccccg acgggaagac gctcatcctc tcgcgcgacc gcaagggcga ggagaacccc
    44761 ggcctctacc tgatgccggc ggacggcggc ccgatgaagg tcgtccagca ccttccgaag
    44821 gtccagacct ccttcgcgtt cgtgagcgcg gacgggaagt ggctctactt ccgatcgaac
    44881 gacgtcaagc cgagctcgta tgccgtctac cgctacgagc tcgccaccgg gaagaaggag
    44941 accgtcttcg acgagccggg gctctggttc gtcgtggaca accggcccga tgaccggatc
    45001 ctcctgatga aggcgaccgg ctccatgacg ttcgagtact gggagtggga cccggcgaag
    45061 cggacgaaga ccccgatgct cgggaccgag aagcccggcc ggtacatcgc ccggtatggc
    45121 ccgaaggagg gccagctcgt cgtcgtgacg cccgagttcg gcgagttcgc gcgcggctac
    45181 ctcttcgagg tgggcaagtg gactccgatc actcccgagc tgagctggga cgtcgaacgc
    45241 gcgggaatgg acgacaagcg gacgagggcc ctctacacgg tcaacgaggg aggctactcg
    45301 aaggtcttcg gcctcgatgc cgcgacgtgg aagccgctcg agatgccgaa gctccccgcc
    45361 gccgaccaga ccaccttcgg ctcgtccacg cccgacgggc ggttcaccgt cttcgccgtc
    45421 ggcaccccga ccgcgcctcc tcagagctgg gtctacgact ggcaggagaa aaggctgacg
    45481 cggtgggtcg tcccgagcgc gcccgaggtc gacacgaagt cgttcgcggt cgcgacgctc
    45541 gagcactacc cggcgcgcga cgggacgaag atcccggcgt tcgtccggcg accgaagagc
    45601 tgcgacccgg ctccctgccc ggtcgtcgtc ctcttccacg gcggccccga gggacaggcg
    45661 acccccggct tctccccgat ggcccagctc tgggtcgacg aggggtacgt cctcgtcgag
    45721 ccgaacgtcc gcggcagcga gggctacggg aagacctggc tcgacgccga caacggcccg
    45781 aagcggctcg acgtcctgac cgacatcgag gacgcggcac actgggcgcg caaagcgttc
    45841 gccgtcgacg gcaaggcccc gaagctgggc gtcatgggcg gcagctacgg cggctactcc
    45901 acgctcatcg cgatgacgaa gttcgcgggg gcctacgacg ccggggtctc gaacgtcggc
    45961 atctcgagcc tcgtctcgtt cctgcagaac accgctccct atcgccggat actccgcgcc
    46021 tcggagtacg gcgacctcga gaaggaccgg gaggccctcg agaagctctc ggccgtgacg
    46081 tacatcgacc gcctgaagga cccgctcctc ctcatccagg gggccactga cccgcgcgtc
    46141 cctgtcggcg aggcgatcca gatgtacgag gcgacgacga agaggggcgt cccgaccggc
    46201 ctcatcatct tccccgacga agggcacggc gcgcagaagc gtgagaaccg cgtcctccag
    46261 acgggtcaca cgatccagtg gttcgacagg cacctgaagg gaaagggcct cccggcgaag
    46321 taggccgcgc ctccctcgac ggacggaagg ccccggggct cccgctccgg ggccttctcc
    46381 ctttccggcc ccgtccgcgc ccccccgccc cgaacccacc ccccccgcct ccccgtaaca
    46441 ttccggtgca cacccgacga acctgaagga ggaccgatgc ccgtctcccg ccgcctcgcc
    46501 cctctcgccg cgttgctcct cgcgctcccc gccctcggtg agccgcagac cgcctacaag
    46561 ggccacggcg ccgacagcgt ccccgccgag gtgatcgccc gctacgcgcc gaagcccctc
    46621 ccgcccgacc tcgcccggcg catccagtcg atgatggacg tccgcgcgcc cggaatggga
    46681 atcgtctcgc ccgacggctc gaagctcttc ttcggatggt ccgtcaccgg gaccgcgcag
    46741 gtctggcgcc tcgacggccc gaacacgttc cccgtccagc tcacgggcgg cgaggaccgg
    46801 acgacgatcg tcgacgtctt ccccgacggg aagactctgg tcctccagcg ggaccgcaag
    46861 ggggaggaga atcccggcct ctacctgatg ccggccgccg gcgggccgct cacgcctatc
    46921 cagcacctgc cggggatcca gactttccac cagttcgtca gcgccgacgg gaggtgggtc
    46981 tacttcgcct cgaacgacgt caagcccgac tcctacgccg tctaccgctg gaacgtcgag
    47041 acgcggcaga aggaggctct cgtcaccgag ccgggcctgt ggagcgtctc cgaccaccgt
    47101 tccgacggca cgctccttct cgcgaagtgg accggctcgc tgacgtccga gtactgggaa
    47161 tggtcccccg cgacgcggaa gctgacgcct ctcctcggcc aggacagtcc cgaggagtac
    47221 acggccgtct acggcgccgc gccgggccag ctcctcgtcc tgacgccgaa gctcggcgag
    47281 ttccgccggc tctgccgatg ggagaagggg acgctcacgc cgctcaccgg cgacctcacc
    47341 tgggacgtct ccggattcag cctcgacgag gcgaagtcgc gtgtcctcta cacggtgaac
    47401 gaggccggct acacgaagct cttcgccctc gacgcgaaga cgttccagcc ggtccacctc
    47461 ccgaagctcc ccgagggagc ggaccacgtc tacgccggcg cctcgacccg cgacgggcgc
    47521 ttcacgacgt tcggcgtcga gacggccacc gcgccgcgca cctcgtacgt ctacgactgg
    47581 cagagcggcg ccctcacccg ctgggtcgtc ccgagcgccc cggagatcga cacgacgacc
    47641 ttcgccgccg cgacgctcga gcactacccg gcgcgcgacg ggacgaagat cccggcgttc
    47701 gtccggcggc cgaagagctg cgacccggcc ccctgcccgg tcgtcgtcga gttccacggc
    47761 ggcccggagg ggcaggccca gcccggcttc tcgacctggg cgcaggtctt cgtcgacgcc
    47821 ggcttcgtct tcgtcgagcc gaacgtccgc ggcagcgacg gctacgggcg gacctggctc
    47881 gacgcggaca acgggcggaa gcgcctcgac gtctggaccg acatcgagga cgccgcgacc
    47941 tgggcccgga aggccttcgc cgcgaacggg aaggagccga aggtcggcgt gacggggggc
    48001 agctacggcg gctactccac tctggtggcg atgacgaagt tcgcaggcgc gtacgacgcc
    48061 ggggcctcca tcgtcggcat ctcgaacctc ctcacgttcc tcgagaacac cgccccctac
    48121 cgccgcatcc tccggacgac ggagtacggc gaccccgcga aggaccggga ggcgctcctc
    48181 gagctctccg cgacgacgca cctcgggaag ctgaaggcgc cgctccagat ccagcagggg
    48241 gcgaccgatc cgcgcgtccc ggtgggcgag gcgatccaga tgtacgaggc ggcgacgaag
    48301 accggcgtcc cgaccgagct gatcgtctat gccgacgaag gccacggcgc cggaaagcgc
    48361 gacaaccagg tcctcatgct cggccacgcc ctccggttcc tcgagaccca cctgaagggg
    48421 gtgccggcga agtagcgccc cgaagaaccg ggggaattgg ggtctggtct acactctaca
    48481 ctctgctcgg agcagagtgt agagtgtaga ccagacccct tcccatcgac cgggccccac
    48541 tcatgtccgc ggctccttcc gcccgaattg gatggcctgc ccgtccctga aatggctctt
    48601 gaaaacgaaa atggatgact catactgtca ttcagatcag gccaatctga agattggccc
    48661 gtcgcagagg ccgaaaggga ggcggagctt ggacatccgg atcgatcgcc agagcgccgt
    48721 ccccgtctac ctccagatcc ggggcggcat tcgcgacctg atcctctccg gacgcctccc
    48781 cgacggcttc cggctccctc ccgagcggac gctcgcgaag gcgctcggcg tcaaccggac
    48841 gacgatcctc tccgcctacc gggagctgaa gaccgacggc ctcgtcgagg cgcacgtcgg
    48901 gcgcggcacg gaggtcgcct cgcagcaggt tctgaagagc gaagcggagc cggccggacc
    48961 gctcccctgg cggcagctcg cccgcgacgg gggcgtgcgc gagcccgatc ccctcgtccg
    49021 cgacgtcctc gcgctgaccg agcggcacga cgtcatcagc ctccaggccg ggcttcccgc
    49081 ggccgagctc ctcccgctcg cggagcttcg gcgcgtgcag gaggagatcc tcgtccggaa
    49141 ggggcccgag gctctgcttc acagcccgac ggaaggggtg acggcgtttc gcgaggcgct
    49201 ctgccgccac atggtcccgc gcggaatccg gacgagcccc gaggaggtcc tcgtcacgtc
    49261 gggctcccag cagggtctcg acctcgtcgc gcgcaccctc ctctccccgg gcgacctcgt
    49321 cgtcgtcgag gagccgtcct acttcggggc gctcgaggcg ttccgctcgg cgcgcgtccg
    49381 cctcctcggc gtccccgtgg acgccgaggg gatgcggacc gacatcctcg aggcgctcct
    49441 ggcccggcag aagccccgcc tcgtctacac gctcccgacg ttccagaacc cgtcgggcgc
    49501 cgtcctctcg cccgcacgac ggcgccacct cctggagctc tcgtggcgcc accaggtgcc
    49561 cgtcctcgag gacgacacct actccgacct ccgctacgag ggttcgccgc tcccgtcgct
    49621 gaaggcgctc gacccgtacg ggcacgtgat ccacctgagc tccttctcga aggtcctctt
    49681 cccgggcctc cgcctcggct ggatcgccgc tccgcggccg ctcgtccgcc agctcgcgct
    49741 cgtgaagcag acggtcgacc tccactccgg gactctcggg cagtggatcg tggaggggtt
    49801 cctctcctcg gggctcctgt cggcccacct cgcccgggtc cgcgccgcct accggcggag
    49861 gcgcgacgtc ctcctcgagg cgctcgcgaa agaggccgtc cccgggctct cctggagccg
    49921 gccggagggg ggcttctacc tctggtgccg cctgccggac gacgtctcgc cggcgagcct
    49981 cgtcgcccac gccgcagccg agcgggtcgc ctacctcccg ggagcggcct cgttcgtggc
    50041 ggagccgtcc gagagctcga tccgcctcaa cttcacgttc gcccccgagg acgacctgcg
    50101 cgagggggcg cggcgcctcg ggagcgcgct ccgcgccgcg caggccgagc cgcgccggcc
    50161 gagccggtcc gtggtgggca cgccgcccat cgtctgagct tcaatcgaac gccgggggcc
    50221 gcgaggccgg cggcgacaag ggggacccca tgacgacccg attcgcacga cggatggaag
    50281 ccctgaaggc ctccgagatc cgcgagatcc tgaaggtgac gcagcggccc gaggtgatct
    50341 cgttcgccgg cgggctgccg gcgcccgagc tctttcccgt caagcgcctc gccgccgcga
    50401 cgcagcaggt cttcgaggcg gaagggccgc gcgctctcca gtactcgacg accgagggac
    50461 acccgccgct ccgccggaag atcgccgaga ggatgaaggc gacctgggca gcgcaggtga
    50521 cgcccgagca ggtcctcgtc acgtcgggct cccagcaggg gctcgacctg accggcaagc
    50581 tcttcctcga cgagggggac gtcgtcctct gcgagagccc gacctacctc ggcgccctct
    50641 cggcctggaa cgtgatgcgc ccgcggtggg tcgaggtcgc gaccgacgac gacgggatgg
    50701 accccaccgc gctcgaagcg aagctccgcg cccacccgaa cgcgaaggcg atctacgtca
    50761 tcccgacctt ccagaaccct tcggggcgca cctggtcgct cgatcgccgc cggcgcttca
    50821 tggaggtcgt cgaccggcac gacgtctggg tcgtcgagga caacccctac ggcgagctgc
    50881 ggctcgaagg ggagtccgtc ccgtcgctga agtcgctcga cacgaaggga cgcgtcgtcg
    50941 ccctcggcac gttctcgaaa gtcttctgcc cgggcctacg gatcgcctgg gtcgccgccg
    51001 agctctccgt catcgagagg ctcgtcatcc tcaagcaggg ggccgacctc cagacggcga
    51061 ccctctccca gctccaggtg aaccgctatc tcgaggagaa cgacctcgac gccgacatcg
    51121 cgaacatcat cccggtctac cgcgagaggc gcgacgcgat gctcgccgcc ctcgagaagg
    51181 agatgccggc cggcgtccgc tggacgcgtc ccgagggcgg cctcttcctc tggatgacgc
    51241 tccccgcgaa cctgaacgcc cggacgctcc tcgacgagtg cgtgcggcag aacgtcgcct
    51301 tcgtccccgg cggcgccttc ttcccgaacg gcggcaacga gaacaccctc cggctgaact
    51361 actcgaacat gccgatcgag cggatccgcg aaggggtgaa gcgcctcgcg gcctccctcg
    51421 tgaaggagat ggagcgagcc ggagtcctcc aggaggtccc ggcgactcct tagcttcgtc
    51481 gcgttccggc gcgcgtcccc gcgtccctcc cctcgagccc cccggcggca ccaccggggg
    51541 gctcttcccg ttccggcgct gaagtacgat gcttcctccc gcgccggccc cggtgcggga
    51601 ggagccgacg atgcgccgca cgatcctggc gacctccctc gccctcctcg ccttcacgct
    51661 ctccgcggcc gcgcaggatt ccctgatcct cgccacgacg acgagcacgc aggacagcgg
    51721 tctcctcgac gacctcctcc cccgcttcga gaaggcgacc ggaatccgcg tgaagacgat
    51781 cgccgtcggc tccggcgagg cgctcgcgat gggacgccgc ggcgacgccg acgtcctcct
    51841 cgtccactcg aaagccgccg aggacgagtt catggcgggc ggtttcggcg cgctccgcct
    51901 cgacgtcatg tacaacgact tcgtcctcgt cgggcctccg tccgatccgg ccggcgtccg
    51961 cggccggccg gtcttcgagg cgatgacacg gctcgccggg acgggcaacc ccttcgcctc
    52021 gcgcgacgac cgctccggga cccacgcgcg cgagctcgac ctctggaagc aggcccgggt
    52081 cgacccgcag gggaagacct ggtacgtcgc gacgggccag gggatgggcg agaccgcgcg
    52141 gatcgcctcg gagaagaagg cgtacacgct cgccgatcgc gggacctacc tggctctccg
    52201 gaagacgctc gacctcgaca tcctcgtcga gggggacgcg tcgctccgga actcctaccg
    52261 cgtcatcgtc gtcagccccg agaagcaccc gaaggttcgc gccgccgagg cccggcgctt
    52321 cgcggagtgg ctcgtctccg aagaggcgca gcgggcgatc ggcgcgttcg gaaaggagcg
    52381 gttcggccag ccgctcttcg tccccgacgc gaagaaggga tgacgggcgt cctcgcctcc
    52441 ctcggcgcgc tcctcccggc gccgggagag ctcctcggga tcgtcctcct ctccctcgtc
    52501 gtctccggcg tcgcgatcgt catctcgatg ctcgtcggcg tccccgtcgg gatcttcctc
    52561 ggcctccacc gcttcccggg gcgcgccttc ctcgtgacgc tcgtgaacac cgggatgggc
    52621 ctcccgcccg tcgtcgtcgg tctcggcgtc ttcctcctcc tcgcccgctc cgggccgctc
    52681 gggggcctcg acctcctcta cacgccgggc gcgatggtcc tcgcgcaggt cgtcatcgcc
    52741 acgcccctcg tcgtcggaat cacgctcgcg gcgatccagg ggctcgacga gagactccac
    52801 ctccagatcc tctcgctcgg cgcctcccgg acgcagctct acctgaagct cgtgaaggag
    52861 gcgcggctct ctctcgtcgc ggcgctcgcc gccggcttcg gctcgatcat ctcggaggtg
    52921 ggcgccgtga tgatggtcgg cgggaacatc cgggggcaga cgcgcgtcat gacgacggcg
    52981 atcgtcctcg agacgcgcca gggggagttc gcgagcgcga tcgtcctcgg cgtcgtcctc
    53041 ctggcgctcg cgctcgggat caactggctc ttcacgtggg tccagcagag gaagccggcg
    53101 tgagcctcgt cgcgctccgg ggcgtcgagg tcgtccgcga cgggaagacg atcctctcgc
    53161 tcgacgggct cgcgctgcgc gagggggagc tcctcgccct cctcggcccg accggcgccg
    53221 gcaagagcac cctcctgcgc gtcctccatc tcctcgatcg gcccgccgcg ggggtcatcg
    53281 agtggcgtgg cgtccccgtg gcgtggccgg ctccgatcga cctccggcga cggatcgcga
    53341 tggccttcca ggacccgctc ctcttctcgg ggacgacgct cgagaacgtc ctctacggcc
    53401 tctcggtgcg aggcgtcccg cgcagcgccg caagggagaa ggcggaggag gcgctccggc
    53461 tccttcgggt cgaccacctc tcccaccgaa gggcccggac gctgtcgggg ggcgaggccc
    53521 agcggacggc gctcgcccgg gctctcgtcc tccgccccga gctcctcctc ctcgacgagc
    53581 cgttcgcggc gctcgacgcc ccgatccgcc ggcacctcct cgaggagctg acgcaggtcg
    53641 tgaaggcgca cgggatcacc tgcgtctacg tgacgcacga gcagggagag gccttctccg
    53701 tcgccgaccg gatcgccgtt ctccgacgcg ggcagcttct ccaggcgggc gcgccggagg
    53761 atgtcctgct tcgcccggcg agccgtgccg tcgcgcgctt catgcagacc ggaaacgtcc
    53821 tcgccggcgt cgtctcccgc cggaccgaag ccctttccga ggtcgtcgtc ggcgagcggc
    53881 tcctcttctc gcgcacggtc ctcgcgaaag ggacgcccgt cgacgcctgc gttcggcggg
    53941 aggagatcct cgtggagccg cccgccggcg gagcccccgc gccaggcctg aaccgatttc
    54001 ccggcgccgt ggagagcgtc gtcgaccacg gcgcgacgct ccacgtccgg atcgacgccg
    54061 gcttccccgc cggcccgctc caggccctcg tcacgcgccg cgtcgcgcac gacctcgccc
    54121 tccgaccggg ccatcccgtc gaggcgacct tctccgcatc ctcggtccac ctcatccctc
    54181 gcgaagggag ccccgatgac gacctgtcgt gaagccctcg acctgatcct cgcctcggcc
    54241 gctccgctct cggcccggcc aacgggtctc ttcgacgccc tcggcctcgt cctcgccgac
    54301 cgcgtcgtct cccccgagac cgtccctccg ttcacgaact cggcgatgga cggctacgcc
    54361 gtgaggcacg acgacctctc tcccgcgtcg gtcgcgtcgc ccgtcctcct cgacgtactc
    54421 gccgaccttc ccgccgggag catcgcggcg cacgaggtga ggtccggcac cgcgatccgg
    54481 atcatgaccg gcgcgccgct tccggccggc gccgacagcg tcgtccccgt ggaatcgacg
    54541 ctctccgagg ggaaccgcgt gcggatcacc gcccccgtga agcggggcgc gaacgttcgg
    54601 ctcgccggcg aggacctccg cgaaggcgag gagctcctcc cggccggttt cgtcctcacc
    54661 cccgcctcgc tcggcatcct cgcctccgtc gggatcgccg aggtcgccgt gaggccgcgc
    54721 gtccgcgtcg cgatcctgac gacgggcgac gagctcgtcg acgcctcggc gaagccgggg
    54781 ccgggacaga tccgcgactc gaacgtccag acgatgtgtg gccaggccct ggccttcggc
    54841 gcgacccccg tcccgatccc gcgtgtcccc gacacgcgcg aggcggtgac ggccgccctc
    54901 gcccgcgccc gcgcggaggc cgacgtgatc gtcacgaacg gcgggatctc cgtcggcgac
    54961 tacgatttcg tcaaggcggt cctcgccgaa gccggcgccc gcgaggtctt ctggaaggtg
    55021 aagcagaagc cgggcggccc gctcggattc tggctctgga acggcgctcc ggtcttcggc
    55081 gtccccggca acaccgtcgc cgccatggtc tgcatggagg agtacgtgag gccggcgctc
    55141 cggaagatga tggggcgctc gaagctcctc cgccccgagg cgacggcgac ctttgagggg
    55201 gatttccgca agtcggcgcc cgacggcaag gtccacctga tccgcgtccg cgcccgccgc
    55261 gacggcgatc gcgtcgtcgt ctccccctcc ggcccgcagg gttccggcat cctctcctcg
    55321 atgctccgct cgaacgccct cgccctcgtc cccgccgacg cgacggagct gaagtcgggc
    55381 gacgaggtcc tcgtccacct catcgaggag cccgaggacc gctgacgccc cggggctggt
    55441 gcccgccccc aggggaatgg tgccacgggg aatggtgcca ggcgtgaaat cgtgtattgt
    55501 tagaagcaat acacgatttc acgcctggca ccgagcgggg gggggcaccg agcggggcgg
    55561 tccgccgggg ccgggaaggg gggtaccctg ctcctgtcgt cccgccggga cggcccgggg
    55621 ccggctcgcg ccgacccctg ccgcccgcgc acctcagcgc gcgatgagga cgcggacctc
    55681 ggcgaggttc cgcaccgagc ggtcgacgaa gaagtcggcc cgcgcgtacg actcgagccg
    55741 ccccgacttc cgaaggagcg gcttgccccc ctcggtgtcg acgaggagga cgttctccgg
    55801 gagacgcgag aggaggacct cgccgccgga gtagagcgag cggtagccgt cgctcgccgt
    55861 gacgacgacg aagaggagcc ccgggtcggt cccggcgggg agcttcgact tgaggagcgc
    55921 cgcgaggctg gccccttcga aggaccggtg cccgtgaaag ccccgcccga ggccgatccc
    55981 gtcgtccgct cccccggcgc gcgggacggc cttcagggcc gcgacggtga gcggcgtcga
    56041 cgagccgtcc gggaggacga atgccggcgc ctcgacccac gggtccttga ggtcacgctt
    56101 ccgcggcggc gccgggaagc cggcctgccg gacctcgatc gagacgacgt cctcgacgaa
    56161 gcggcgtccc gtcgcgtcgc gcgtcgggac gaggcagagg ccgcgcggga cgtcgatctt
    56221 cccgagcttg ccgccgtcgt ggcaccccgc gcagccggag acgtcgagct tctcgcgcgc
    56281 ctcgacgccg aaccagctcc ccggcgcgaa gcgtgggtct tcgatcgcct cgtggtggtg
    56341 cgggacgagc ggccgcatcc tctcgacgag gagcgccgcg cccgcgtcgc cgaggaggaa
    56401 gagctcgccc cacgagaaga gcgccttctt cccgtcccga ccggtcacca cgaccgcgag
    56461 gtcgagcgga cggtcgaagt tgtcgttcgt cgccttcgcg acctcggcgc tctcgaggag
    56521 ctccttcagg gagacccctt cggtctcgaa cgtcccgcgg taacgcgatg cccccggcac
    56581 cttcgcgagg acggcgtggc gcgacagggc ggcaagccgg gcgccgtcga cgacgccggc
    56641 cttcttcacc gagccggaga gggcgatccc cttcggcgcc gccgggaccg ccgccgtggc
    56701 cggctcctgg gcgcgaagcg gacggagcga tgccgccgcg acgaggacgg cggcgacgag
    56761 aaggacgctc ttcgaggctc gggtcacttc ggacctcccg cgggcggccg cgccgccccg
    56821 cgatgtcgct gctttcgaga cggcggcctc agccgccgct gatgagatgg acgaccttga
    56881 cgtcagcgcc ggccggaacg acgaagtcgt cgtacgaacc acgcggggcg agggtgccgt
    56941 tcaccgtgac gatgaggagc ttgaagacgt agttcttcgc ctgcagcacg tcgcggacgg
    57001 tcatcccctc gcgccagtcg agcggctcgc cgttcacggc gagccgcgtg tctcgatccg
    57061 ccgccggcgg cgcgccgggg tccgcgccca ttcgctcagc ccccgtgctg cttcacgagc
    57121 tcgacgagct cgggaagggc gatgccgagc ttctcgagcg tcgcgacggt cgggacgccg
    57181 tccgtcgtcc agccgcgacg cttgtagacc gcgtcgcaca gcttcgagta gcggtcctcg
    57241 cggtactgcc tcgtgatcgc gatcttctcc tcggtcgtct tcccctccgg gtcgacgccg
    57301 atctcctcgc gcatctgctt gtcgtagcgc tccgcgcgcg actcgtactc ctcgaccgtc
    57361 accgggccga cggagcggta cggcggccag tcgtgctcgc gcttcccgta gccgcgccgc
    57421 aggttgaaga tcctctggaa gttgtagacc ttctccgact gctcgatgat cttctccttc
    57481 gtgacctcga tcccggtcac gccggaggcg atctcgcagt agttgtggac gtgctcgggc
    57541 accttggccg gctcggcctg ctcggcgttg ttcgccggct cgatgtcgtt ccaggggagc
    57601 ttgcagagcc cgttcaggcc gaaccacgtc cggaagagcg ggaagtagtg gagcgcctcc
    57661 gccttcgcct cgaacgtcgg caggtgcttg ttgaccatgt ccatgaagat gagccacgcc
    57721 tcgtcgtgct gggggccctt gttcgtcagg gcgtagcccc cctgctgcgc gagcgactcc
    57781 ttcgactggt actgcgagta ctcgagcccc ttgacctcca tgccgatgtc gttcatcagc
    57841 ttcgggtcgc cccagccgtt ctccgcgaag atccgcttca tctggcggat ccccttcccc
    57901 gcgatcacgc cgaagccctc gccccgcgcc atctggtgga ggagctccat ctgcgcctcg
    57961 gcgttccccc atgtcaggtc gaggccgccc gttcgctcgg cgttcaggat gccccgctgc
    58021 cagcattcca tgacgaacgc gcagagcgtg ccgaaggaga tcgtgtcgac gccgtaggtg
    58081 tcgcagtaga agttcgactc gaggagccag tcggggtcga agatgccgca gttcgccgcg
    58141 aggccggcgg cgttctcgta ctcggggccg tctaccgtca cgacgtcgcc cttgtagggc
    58201 cccgtcttca cgacgtgccc gtccgctccc ttcgagcacg acatcgtgca gccgtaccag
    58261 cagccgtccg ggatcccctg cgtgaagcgc ttgtcccaga cccacgacgc gatgttcttc
    58321 gtgtcgggat ggttgccgta ctggaagttc ttcaccggga ggaggtcgta ggcgtccatc
    58381 acctcgacga ggtgggccgt cccctggcgg cgcatgttgc actgcgagga gtcgaggtcg
    58441 tggatctcct tgttgatgac gctcccgacc ttctggattc gagcccggtc ggcgacgtgg
    58501 ttcaggtcgg ccttgatgac cggcccgcgg acgacgaggg ccttgagctt cttgtcgcgg
    58561 aagaccgtcc cgaccccgcc gcgccccgcc tgcttcaggc gcgcggcctt cctgcggagg
    58621 tcccagaagg agaagttgag gcagccgatg tacgagtggt ccgagccctt ccccgccgag
    58681 acgacggaca cgttcttgta gtcctcgggg cccgtcgcgt acatgtgcgt gaagatctcg
    58741 gagaggacgt ggctgtcggg cggctcgagg gggcactcct cgatccggat gacgccgcgc
    58801 ggaccgtcga cgacgacgac gacctcggcg gccgccttcc cctggatctc gatcgcgtcg
    58861 aagccgcaga acttcaggag cggcccgtag tagccgccga cgttgcagtc gatcgggatg
    58921 tccgtcaggg gcgagatcga gacgacgagg gacttgccgg tccccgggta ctgcgtgatg
    58981 ccggcgatcg gcccgggggc gatgacgatc tcgttctccg ggtcgttcca gcgagtcgcc
    59041 ggcgtcgtcg cgttccagag gtaccagagg ccgaagccgc gcccgccgac gaagaggtcc
    59101 ttcatctgct gcgtgacctt cttctcggtc accttcatgg ccgtcaggtc gacgtggagc
    59161 gacctcatgt tgtagccgcg ctccggctcg ctcggcgtga acggcacctc gaggaggacc
    59221 ttgtgggccg ccctcatctc ctcgaggccc tgcgtgtagc tcgtgcggac ggtgtagtcg
    59281 cgcgcggtcg aggtcgtcgg ggaatcggtc gcagggcttg tcgacatgct cgcctcgtcg
    59341 cggccggccg gcgcgccccg aaggggcgtc gggaagggcc gaccgctttg gaacggtagc
    59401 atcgggcatc gaagcggggc aaggaaaggc cggattgcac cggacgttct cgcgagagaa
    59461 tccgtcgcga tgagtccccc gcacggcgac cgatattccc gccaggtcct cttccgcccc
    59521 ttcggggccg aaggccaggc ccgcgtccgg gccgcccgtg tcctcgtcgc cggctgcggc
    59581 ggcctcgggg ccgagtcggc gagcctcctc gcccgggccg gcgtcggctt cctccgggtc
    59641 gtcgaccggg acgtcgtcga gctctcgaac ctccagaggc aggcgctctt cgacgaggcc
    59701 gacgtccgcg aggggctccc gaaggcaatc gccgccgcac ggcggatcgg ccgcgtcaac
    59761 gcggacgtcg cggtcgagcc ggtcgtcgcc gacgtcggcc cggggaacgt ccggagcctc
    59821 ctcgacggaa tcgacctcgt catcgacggc ttcgactcgt tcgaggcccg cttcctcctg
    59881 aacgacgcct gcgtcgagaa gggcctgccg tgggtctacg gcgcctgcat cgccgcgacc
    59941 ggactcgcgg cgctcctcgt cccgggcgtc acgccgtgcc tccgctgcct cctcggcgag
    60001 atccccgagc cgggctcgtc cgccacctgc gacacggcgg ggatcctcgg ccccgcggcc
    60061 cacctcgccg cctcgatgca ggtcggtcgc gccctccgct tcctcgcgac gcgcgaggca
    60121 ccgcccgagg ccgagctcgt ctacgccgac gtctgggagg cccgcttcac ccggatggcg
    60181 gtgccccgcg acccggcgtg cccctgctgc gccggccgcc gcttcgactt cctgtcggtc
    60241 gagacgtccc ccgcctcggc cctctgcggc cgggactcgg tgcaggtccg cccgctcgtc
    60301 ccgtcgaggc ccgacttcgc cgcgctcgcc gagcgcctcc gccctctcgg ggaggtcgtg
    60361 tcgaacgact acctgctgcg ctaccgcgcg gacgacgtcg agatgaccct cttctccgac
    60421 ggccgcgcga tcgtgaaggg gacgacggac cccgcacgcg ccaggagcct cgtggcgagg
    60481 acgttcggag tgtgaggcgc gccaggatgc gtccctggct cgtctacggg ttcctcctcg
    60541 cgctcaaggg gctcgcccgc ctcttctacc gcgtcgagat gacttgggtc ggcgaccctc
    60601 cgcccgaccg gtgggagcac ctgcggctcc tcgtcttcct ccaccacacg agcctctacg
    60661 aaccgatctt cctcggaggg gcgccgaacg gcttcatccg ccaggtcgcc ttccacggcg
    60721 tcctgccggc cgccgagaag acgaccgccc gcccgatcgt cgggatgctc ttcaggatga
    60781 tcgccgcgca ggtcgtgccg atctcccgcg cccgggacca cacgtggttc gaggtcctcc
    60841 ggaagatcga ccccgagtct atggtcgtcc tcctccccga gggccggatg atgcggacga
    60901 acgggctcga cgcgcacggc cggccgatga ccgtccgcgg cgggatcgcc gacgtcctcg
    60961 aggcggtcgg cgagggccgg atgctcgtcg cctacagcgg gggcctccac cacgtccagg
    61021 ctcccgggga gcgggtcccg cgcgtcttcc ggactgtccg gatgaggctc gagctcctcg
    61081 cgatcgctcc gtacgtccgg gaggccctcg acgggcgcgg cccggacgag ctgcgcgagg
    61141 ccgtctgcgc ggacctggaa cggcgccgct ggctccactg ccccgtcgcc ggcccaaggc
    61201 cctggccgga gcccgcgaac ccgatccaga cctgagacgg cgccctccgc ccggggagcg
    61261 acccggcccc gcctctccct cgcgcgcggg gcgccgagcg ccgccgcgag gccctgcgag
    61321 gcccgaagag gagccgcccg tcactcgcgg cagcgcagga gggccccgtc gaggtcggcg
    61381 cgcgcgaggc gaaggcgggt gacgagctcg agctccttca cgcggtgcgg gaactcgagg
    61441 atgtaagttc cctcctcggg cacccaaacg acgcgagggt gcaccagccc ggcggccttg
    61501 agcgcgacgg cgggctcgtt cgcgagcgcc gtgtgatccc aggcccgctg gaggatggcg
    61561 cgcatctcgg gagggcactt cgtgaacgtc ccgcccgccg acggcacggt gaggacctcg
    61621 aaccacgctt cttccgcgta gctgtagacg tggagggacg gctccgcgat cgtcgtcggg
    61681 aggtcccggt ggtagaggtt cgtcgcgaag acgaacgagt cgtccgcgta cgagacgatc
    61741 atgtgatggc cgggaagcat cgcttccttg cagtcgtagc gtcgccacgt gcccgcgtgg
    61801 ggagagtagg cgaggatgcc ggcgcccttc gtcccgaccc agagctcctc gttccccaca
    61861 gccatcccgc tcgtgtcgtc gagcatgcgg tgcgaccccg ggtccagcgg ccagcccgcg
    61921 ctctcggcga gctcggaaat cgcgatccac cgttcctcac cggtccgctc gtcgcggagg
    61981 tggaggccgg agccctgctg ctgaaacgtg taggggccgg cctgcaacga cgggccgaac
    62041 aggtacgagc accgggagcc gccgcggtag ggcacgaccg tcgccggggc gacgaggcgg
    62101 atcgagctcc ttcgcacggt gaccgtatcg ttcggcggca cccccgccgc cgcgatcgtc
    62161 acgaaagcga agagcgcgat cggaacgagg catcgtgcgg agagcgacac ccgtcgcatg
    62221 tgctcatgat gctcctctcg gccggctcgg cgcaccggga gaacgcgggg ccggagcgcg
    62281 aggagccgcc ccgggcacgg cgccgggcgc cgggtccctc actcccgggt gagcgtcctc
    62341 cgctcgagaa gcgagtagtg ccgccacgag ctgctcatgt gcgaaccgac ggaggcgagg
    62401 agcgtcgcgg cgaggtagag ctcgaagcgc gcggcgggat acgcctgccc gatcacgagg
    62461 agtgcgagct tgaggagggt gacggcgccg gcgagcatgt agaggtagac gcccgaccgc
    62521 gcgatctcga ccgcgaggag cccggcgccg ctcgcgagcg tgacgatccc ggcggtgtgg
    62581 agcctcgcgt ccgggacgtc gaacgcgagg ccgccgagga cgaccgccat cgccgcaacg
    62641 tggagcgtcc ggagcgcgac ctgcacgggc cgggcccagc ggaggtcccg cggcggccag
    62701 gggaagaggc cgctcctccc ggtcccctcc cgcaccccgg cgcccgcacg ccgcctctcg
    62761 ccctctcgca ttccggctcc tcgctggcga gccggcgcct cggagccggc tcgaccgggc
    62821 ggatcgtctc gcgaggcgtg acccgctggc aaaggggccc cggccggtcc ggaagcgggc
    62881 acttcattcg agccgctccg gtcccgtacg gattctccga gcttcactcc gtacgcggcg
    62941 gggactacga ttcccggtcg gactccacga gcccgcgccc ccgcgagaga accggaggac
    63001 cgtcggaaat gaggactcac ggaacggacg ctcccgcgca cggcccgctg tcgctgcgcg
    63061 cggagatgaa ggagcggacg gggaagaaca ccgcccgctg ctaccagtgc gggaagtgct
    63121 ccgccggctg cccgatggcc ggagagaccc ggctccgccc gcacgacgtc atgcgcctcg
    63181 tgaaccagga cgcgcgcgcg cgcctcctcg cggacgagtc gatctggctc tgcctgacgt
    63241 gcgagacctg tacggagcgc tgcccgaacg gctgcgaccc ggcgcggacg atggacgccc
    63301 tccgcgagat ggcgctcgag gcctcgcccg gctcgccgcc gcgcgccctc ctcgccttcc
    63361 accgcgcctt cctcgaccag gtgcggtgga cgggccgctc cttcgagctc ggcctcgtcg
    63421 ccgactacaa gctccgcacg ggcgcgctcc tccaggacgt cgcgacagcg cccggtctcg
    63481 tcgcccgcgg gaagctcggc ttcgtcccgc acccgatcga cggcgtgaag gaggtccggc
    63541 ggattttcga ggcgtgcgaa gaggcgaccc gcgccgcctc cgggaagaag gagggcgcgt
    63601 gagagtcggc tactaccccg gctgctcgct ccacggaacg gcccgggagc tcgacgagag
    63661 cctccgggcc atcgccccgc cgctcgacct cgagctcgtc gaggtgaacg actggtcgtg
    63721 ctgcggcgcg acctcggccc acgcgacgag ccatctcctc tccgtggcgc tcccggcccg
    63781 caacctcgcg ctcgccgagg agcaggggca cgaccagctc ctcgccccct gcgccgcctg
    63841 ctacaaccgg ctcgcgagcg cccgccacgc cgtcgcgtgc gacgcggcgc tcgcggagac
    63901 gatcgaggac gtcctcggcc gccccttccc ctccacggtc gcgccgagga acctcgtcga
    63961 ggtcctccgc gcgcggctcg acctactccg cgagcgcgcc gtccggcccc tcacggggct
    64021 gaaggtcgcc tgctactacg gctgcctcct cgtccgcccc tccgaggtct gctccttcga
    64081 cgacccggag gacccggtct cgatggagga ggtcgtcgcc gcgacgggag cgacgcccgt
    64141 cgcctggcgg cgccgcctcg actgctgcgg cggcgccttc tccgtcgcgc ggaccggctc
    64201 cgtcgtccgc ctctcgcgcg cgatcctcga cgacgcgcgc tccgccggcg ccgacgccat
    64261 cgccgtcgcc tgcccgatgt gccactcgaa cctcgacttc cggcagaagg ctctcgtgcg
    64321 gcgcggcgag acggagctcc ccgtcctcta cctctccgag ctcgtcggcc tcgcgctcgg
    64381 cctcgacgcc ggctcgctcg gcctcgggag gcacttcgtc gagacgaagg gcttcgtgga
    64441 gcgcgccctc gcgccgaagc ccgccgcgaa gccgccggcc ggcgcggcga agcccgcgac
    64501 gccgaagccg gccgcggaag cgacggccgg gaaggagacc gtctgatggc tcgcatcggc
    64561 gtcttcgtct gccactgcgg cgagaacatc ggccggacgg tcgactgcgc ccgcgtcgcc
    64621 gaggagctcg ggaagctccc gggcgtcgca cacgccgtcg actacaagta catgtgctcc
    64681 gacccggggc aggcgctcat caagcaggcg atcgccgaga aggggctcac gggcgtcgtc
    64741 gtctcggcct gctcgcccca catgcacgag aggacgttcc gccgcgccgc aggcggggcg
    64801 ggcctcaacc ccttcctctg cgagatggcg aacatccgcg agcactgctc gtggatccac
    64861 gaggaccgcg aagcggcgac ggacaaggcg atcgacctcg cgcgcgtcat cgtcgagaag
    64921 gtcaagagga acgtcccgct cgagacgatc cggatcccgg tcgagaagcg ggccctcgtc
    64981 atcggcggcg gcatcgccgg catccaggcg gcgctcgacc tggcggacgg cggcgccgag
    65041 gtcgtcctcg tcgagaagga gccctccatc ggcgggcaca tgagccagct ctcggagacc
    65101 ttcccgacgc tcgactgctc gcagtgcatc ctgacgccgc ggatggtgga ggtctaccag
    65161 cacccgcgga tcaggctgat ggcctactgc gaggtcgacg ccgtcgaagg gtacatcggc
    65221 aacttcaagg tcacgatccg gcagaaggcg cggagcgtcg acgagacgaa gtgcaacggc
    65281 tgcggcgcct gccagaaggc atgcgtcgtg aagaacgtcc cgagcgagtt cgacgagcgg
    65341 ctcgggaaga ggacggcgat ctacgtcccg ttcccgcagg ccgtcccgaa catcccggtc
    65401 atcgaccgcg agcactgcgc gtacttcaag gggagagcgc gcggcatgaa gaaggacgcc
    65461 tgcggcaagt gcaaggaggc ctgcgctcgc ggggcgatcg acttcgccca ggaggaccgc
    65521 ttcgtcaccg agaaggtcgg ggcgatcgtc gtcgcgaccg gctacgagct ctactccatc
    65581 gggaaggacc agcccgccgc cggcctgaag ggctacggcg agtacggcta cgggaccgtc
    65641 cccgacgtca tcgatggcct ccagttcgag cggctcgcct cggcttccgg gccgacggcg
    65701 gggaagctcc tccgcccctc ggacggcaag gtgccgaaga agatcgtctt cctccagtgc
    65761 gtcgggtcgc gcgacccggc gaaggggatc gactactgct cgaaggtctg ctgcatgtac
    65821 gtcgccaagc acacgatgct ctaccggcac aaggtgcacg atggcgaggc gacggtcttc
    65881 tacatggacg tccgggctgc cgggaaggga tacgacgagt tcacgcgccg ggccgtcgag
    65941 gaggacgggg ccgtctacgt ccgcgggcgc gtctcccgcg tctaccggga cggtgacgtc
    66001 gtgaaggtcc tcggcttcga cacgctcaac ggcacgccgg tcgagatcga cgccgacatg
    66061 gtcgtcctcg cgacggcgat gcggccgcgg aaggggatcg agaagctcgc ccagacgctc
    66121 tcggtctcct acgaccggca cggcttcatc aacgaggcgc acccgaagct ccgtcccgtc
    66181 gagacgaaca cggccggcgt cttcgtcgcg ggcgcctgcc aggcgccgcg cgacataccc
    66241 gactccgtcg ccatggcgag cgcggcggcc gcgaaggtcc tcggcctctt ctcgaacgag
    66301 atgctcgagc gggagccgac cgtcgcccgc gtcgacgccg cgctctgcgc cggctgcttc
    66361 cactgcgaga gggtctgccc ctacggcgcg gtggagcaca aggaggtccg cgaccggaag
    66421 gggaacctcc tgaaggtcgt cgccgacgtc aaccccggcg tctgccaggg ctgcggcacc
    66481 tgccaggcga cctgcccgtc gaagagcgtg gagctgatgg gcttcaccga cgagcagatc
    66541 tacgccgaag tgaacgccct cgcgctctga ggacaccgaa atgaacgcca ccctcgacaa
    66601 gacctccttt gagccccgcc tcacggcgtt cgtctgcaac tggtgcacct acaccggcgc
    66661 cgacctcgcc gggacgagcc gcctccagat ggacgcgaac gtcaggatcg ttcggctccc
    66721 ctgcacgggg cggatcgacc cgctcttcat cgtcaaagcg ttcgagaggg gggccgacgg
    66781 cgtcatcgtc agcggctgcc accccgccga ctgccactac acctccggca actaccatgc
    66841 gaggcgccgc ttcgcgttct tcaaggagct gatcgagttc ctcggcctgc ccgaggggcg
    66901 gatcacgttc tcgtgggtct cggcctccga ggggcagaag tggcgcgagg tcgtgaacga
    66961 cgcaacggcc cgggtccggg ccctcggccc gttctccggc tacccgggga cggagcttcc
    67021 cgcctggccc gcggcgggtg cgcccgcgcc gaatcccgcg ccgggcggcg gaggtgagcg
    67081 atgaaggagc tccgcgacct cgccacgaag ctccttgccg aggggacggt gaacgtcgtc
    67141 ctcgggtggg aggaggggcc gcgcggcgtc cgccccgcct tcgcgacgga cgcggccgcc
    67201 gccgggcgcc tcgtcttcga cccccgctgc acgcagaacc tcgtcccgta cctctcgcca
    67261 cgacggacgc aggtgaagaa gctcggcaaa gccgccgtcg tcgtgaaggg gtgcgacgcc
    67321 cgggccgtcg ccggcctcgt ccgcgagacg cagctcgcgc gcgaggacgt cgtcgtcatc
    67381 ggcgtgcggt gcggcggcgt catcgccgac cccgcctctc ccgagccgct ctcccccgcg
    67441 accgtcgcgg accgctgccc cggctgcaac tcgagagagc cgaagctcgc cgatcacacg
    67501 gtcggcgagc ttcccgccgc ccctcccggc gagagccgac gcgacgtgcg gatcgccgat
    67561 ctcgaggcga ggacggccga cgagcgctgg gccttctggc aggacgagct cgggcgctgc
    67621 gtccgctgtc acgcctgccg cgaggcctgc ccgatgtgct tctgcgagcg gtgcgtcgcc
    67681 gacaagacgc agccgcagtg ggtcgactcg tcgcctcacc cgcgcggcgc cttcgcctgg
    67741 aacgtgacgc gcgcccttca cctcgcgggg cgctgcgtcg actgcggcga gtgcgagcgg
    67801 gcctgccccg tcgacatccc gctcggcctc ctgaacctga agctcgcccg agtcgcctcc
    67861 gagcggttcg gatacaccgc gacggacgac cccgccgtcc cgtcgcccgt cggctccttc
    67921 cagaaggacg acgcccagga gttcatcctg tgaacggacg cacgatctcc cgcagccagc
    67981 tcgccgccct cgtcgacgac ctcgtcggct ccggcgtcgg cgtcgtggcg cccgtccgcg
    68041 cgcccgacgg ccgcctcgac taccgccccg tcgcgcgcct cgaggaggcc gtcctcgacg
    68101 gcggcctgcc gcgcctctcc ctgaagtcgc tcttcctccc gccgaccgag ccgctcttct
    68161 cgttccgcaa gagcggaccc gacgtcgcga tcgactccgt cccgacgacg ttcccgcccc
    68221 gcctcgtcct cggcgccgtg ccgtgcgacg ccgcgggcgt cgcggcgctc gacaaggtca
    68281 tgggctggga ctaccgcgac gagctctggt tcggccggcg cgaggcgacg acgatcgtct
    68341 cggtcgcctg cggcaagggc gacgcctcgt gcttctgcgg cgcgaccggg aacggccccg
    68401 ccggcacccg cggcgccgac ctcctcctcc tgcccctcga cgacggcttc gccgtcgaag
    68461 tcgtcacgcc gaagggggag gcgctcctct ccgcccacgc gggacgtttc ggcgagcccc
    68521 cgaacgaggc cgacgtcgcg aggcgccgcg ccggcctcct cgaagggagg gtcgagccgg
    68581 tcggatcgct cgcccccgtc actgcctggc tcggcgggca cttcgacgac cccgtctggg
    68641 acggcatcgc cctccgctgc cacggctgcg gagcctgcgc ctccgtctgc cccacctgcc
    68701 actgcttcga catcgtcgac gagcccgagg gggtgacgcg cgggacgcgc cgccgcaact
    68761 gggacacctg ccagacggcc cgcttcacgc tccacgcctc gggccacaac ccgcgctccg
    68821 tccaggacgc ccgctaccgg cagagggtcc tgcacaagtt ctcgatctac cccgagcgct
    68881 tcggcgagct cctctgcacc ggctgcggcc gctgcagccg cgcctgcccc ggagggatgg
    68941 acctccccga cgtcctcggg gcgatcgccg cccgcgccgc cgcccccgcc ggaggtgccg
    69001 cttgaacctt tacgagccgt accgcgtgaa gatcgccggc ctcgtcgacg agacccccga
    69061 cacgcgaacc tttcgcctcg agttcctcga tcccgaggtc gcccggacgt tcgacttcaa
    69121 gacgggccag ttcggcgagt actccgcctt cggggccggc gagtgcacct tctgcatcgc
    69181 ctcctccccg acccgccggg gctacatcga gtgcagcttc aagaccgtcg gcaaggcgac
    69241 gacggccctg cgcgacctga acgtcggcga cacgatgggc ttccgcggcc cctacgggaa
    69301 ccacttcccg ctcgagagga tggaggggtc gagcctcgtc ttcatcgccg gcggcatcgg
    69361 tctcgcgccc gtccgctccg tcatctggaa cgccctcgac ctcagggacc gcttcaagga
    69421 cgtcacgatc gtctacggcg cccgctccgt ctccgacctc gtctacaagc gcgagctcga
    69481 ggagtgggcc gcgcgcgcgg acgtgaggct ccaccagacc gtcgaccccg gcggcgagac
    69541 gccggactgg gcgggcgagg tcggcttcgt cccgacgatc gtcgagaagt cggccccgtc
    69601 cgcgacgaac gcctacgcga tcgtctgcgg gccgccgatc atgatcaagt tcacgcttcc
    69661 cgtgctgaag aagctcggct tcgccgacga tcgcatctac acgacgctcg agaaccggat
    69721 gaaatgcggc atcggaaagt gcggccgctg caacatcggc cccgtctacg tctgcaagga
    69781 cgggccggtc ttcaccgcgg aggagctcgc gaagctgccg ccggagttct gacggaggag
    69841 ccggaaggcc cgctccccgg aggcggagcg gggcctctcg ctccctcggg ccggccggcg
    69901 aggcctactt cagtgcgccc ttgatcttct tcacctgctc gcgcgagtag tagctgcagg
    69961 cgcctttctt cgcgacgggc gcgagcttca gcgcctcgat cgccttcttc acctgcgcgg
    70021 gggaggcccc cagctccttg gcgatgttcc ccgcggtgat ctgctcttcg gccatcctct
    70081 gctcctcccg ggcacggcgg gcccgacccg gtaggctacc gccgaagccg gccgggcgca
    70141 tccgccctcg ccatctccca accgcggggc ggcgccctgc ccccctcgct tccgcagaat
    70201 cctgctcgcc gggtgtcgta cttttcgccc tctcgcggag cctagccgcg gaaggatccc
    70261 gggccccgcc cggcggttct ccatgagcgc gcgtgacgtc cgccatcgct cggccgccca
    70321 agagtctccc cttacacagg ggcggagagg cctccgcccc cacgaccctc gacgacgacg
    70381 acgggatcca catcgccttc gtgacccgcg ccggctcccc cgaggcgccg atcctccggg
    70441 tcgtcgtctc ctggtgggag gaaggggcct gggtcgagaa agcggtcgac ctcgtcgaca
    70501 ccccgccggg cgaggtcgac ttcccgatcc gcgacgcggc tcctggtcct gcgggcctct
    70561 ccgctccgag ccggacgccg tccaggccga ccagctgatc ctcggcctcg ccctcgcgcc
    70621 gtcccgaacc ttcgcccgct gagtgcggag ggctccaggc acgacgccga ctgcgctgaa
    70681 gggcgctcgg ggggggcatt cgggctcgga cccgacaacc gatgaacgca cttttccgct
    70741 cccgtcctgt aggagttccg accctctcgc ggagcttcgt ttcaggaaag gtcgccattc
    70801 cgttgcctcg acccggggaa ctctcgggtg ggttgtcctc gtccgctcgt accgcgccga
    70861 agaagtccat ccacttcgac tcggaggcgc ggcgagacac gacggcacga gagcgaacgc
    70921 gacaccgaag ggcgcggagc gtcgacagcg cgcgagcccg ctcccacgca acccattcac
    70981 acataacgga atacggggaa gcccccgacc cccgggaacg caggtccctt cgtcttcgag
    71041 gagccaccat gcccaagctc gccgcgccct ccccttccct ccctccgcgc ttccgcgcgc
    71101 atccgggcta cagctcgctt tccgtgcgcg acctcctcga cgcgcgcgag gcctaccacg
    71161 tccacctgag caacatggcg cacgtcgtgg cgacggcgat cggccggtac aggatccgga
    71221 agggggactg gtacgagacg catccgcctg agatcccgcg gccaaggggc cgaaaggaga
    71281 agaaggacgc gagggacctg ttcaactcgg tcacgaaggc ctggtcgtgg ccgtgcgtcc
    71341 tcgtcttcgt cgacgagtgg gtcaagcgcg aagactggaa gagagggaag gcggggcgcg
    71401 gcctggagcc ggaccagttc atcccgcgga gcctctacct ccccgacggc cgggtcgtcc
    71461 cgacgtgcgt cgtcctcctc cggaagagcg acgtccggcc ggaggcgccc cctccgcggc
    71521 ggttccccga gccgctcgtc ggcggcggct acccgctcct gacgaaggtg caggggacga
    71581 gccgggtcgg atccgtcggc tgcctcgtca ccgacggccg ccagaccttc gtcctgacga
    71641 accgccatgt gacgggcgaa gagggagacg ccgtcttctc cctccttggc ggcaaggagg
    71701 tgagggtcgg ccgggcgagc ccgctcgtcc tggggaagag acgattcggg gagatctacg
    71761 atccgtggcc gggaagcagg gtcttcgtca acctcgacgt cggcctcgtg aaggtcgacg
    71821 acgtgtcgat gctcacgacc caggtcctcg gcctcggccc gctcggctcc ccgatcgacc
    71881 tgaaccccga cacgatctcg ctcgacctcg tcgagaagcc cgtccggggg tacggcgccg
    71941 cctcggggcc gctctccgga cgcatctgcg ccctcttctt ccgctaccac gccatcggcg
    72001 ggttcgagta cgtctccgac ctcctcatcg cgcccgacgc gggccgcgag acgcacccgg
    72061 gcgactcggg caccctctgg tgcctcgaca cgcgcgacgc gaaggggcag ggcggcctct
    72121 acccgatcgc cctccagtgg ggcgggcagg tcatcgagga gggcccgtcc ggcggccggg
    72181 cgcgcggctt caccctcgcg acgttcgtca gcacggtcct gcgcgagctg cagctcgacc
    72241 tcgtccgcga ctggaacatc ggcctcccgg agtactgggg agcgctgggg cactactcca
    72301 tcgcgacctt cgcctgcggg ttcgtcacgg gccgactgaa gaccctcatg gggaagaacg
    72361 tcgaccgggt ctcgtacccg ccctcgcgcc tcgagccgtc gaccttcccg ggcctctcga
    72421 agaaggactt cattcccctc gccgacgtcc cggactacta ctggaagatc cccggcagcc
    72481 ccggccgccg ggcgaccgac aacccctccc acttcgccga catggacaag ccgctccccg
    72541 acgcgccgca cgcggggaag acgctcctcg acctctgcat cgaggacccg gcgaacgtcg
    72601 accccgccgt ctggaaggac tactacgagc gcgtcgacga ccggagcaag ggcgtcctcc
    72661 ccttccgcgt cggcctcatc tacgaggaga tggtcgcttc cgtcgcggcg aaagacctcg
    72721 atcgcttcgt ctgcgcggcc ggcatcctct cccactacgt cggcgacgcg tgccagccgc
    72781 tccacatctc cagcctcttc gacggcgagc cgcttcccgg cggcgggaag aggggcgcgg
    72841 gcgtccacca cgcgtacgag gcggacatgc tcaacgcgaa cgcggcggcg ctcgtcgccg
    72901 ggatcgacgc cggcctcgcg gccgccgctc ccctgccgaa cgtgtcgggc gttcgcgagg
    72961 cgaccctcgc cgccgtcgac ctgatgcgga agaccttcga gaacctctcc ccgaaggcga
    73021 tcgtcgacgc cttcgccgac ggcgaggacc tctgggacct cttcggcgac gccacgatcg
    73081 ccacgtgcgt cgagggcgcc cgcaccctcg cgatgctctg ggagagcgcc tgggcggaag
    73141 ggaacggcga cgccatcccc aagtcgaagc tcacggcgcg aaacaaggac gacctcgtga
    73201 cgctctactc cgacgaggag ttcatcccgt cgctcacgct caagcagctg atcgagggga
    73261 cgtagggaga gcgcgggcgc ccgcgacacc cgctccacga cctgaacgct caggaggaga
    73321 cgcgaatggc cgaaacggat tcccgtccta ccgggcctcg cgatgccgac ggcgcgaggg
    73381 gcgcccccgc cccggagtcg cagccggcga ctcctccggg cccgagtccc cgttcgccct
    73441 cctggctgtc ctacaaggtc ggggacgtct gccggtccac cggcgccgtg atctccgaga
    73501 tcctctacag gagcgagaag agcttcatct tcctggatcc ggcggaccgg ctccagtgtg
    73561 aattcgacga gcacgcgtgc atcgatggcg acgactccat ggctcaggcg agcgacctcg
    73621 tctgccaggc tcgactgaac catttcagca ggcgcattcg acgccgggcc ttcggcctcg
    73681 tcgcctccgg gctcacccgg gccctcgaca cgcgtacggc gggagataag accgacttct
    73741 tccgcgacgc ccgcgagttc ctccggttcc ggcgccgcga ggggcttcaa ttcgtctatc
    73801 tcctgtccgc ccttctgagc gtcgtcgcca tctccgccgt ctccctcctg agccagctcc
    73861 tcccgatcga ggcgaacggc cgatccatcc tcctcgccgc ccacttcggc agcctgggtg
    73921 cattgatctc gatctcgcag aggtcccgga agatcgctat ccctcgatac gcctcacgct
    73981 tctacgccgt cgcgggcggc ggctcgagga tcttcttcgg cgcggtcttc ggcgccgccc
    74041 tcctcctcct tcagaaagcc gggctcgtgc tgaacctcct cgactcgaac ccgtacgcgc
    74101 tctatgccgc atcgctcgtc gccggcttca gcgaacggct catccccgag ctcatcggcc
    74161 agttcgagaa gcagctgcgc ccggacgagt ccccgagcgt gccgtccggc gcgccatcgc
    74221 cgcctcctgc cgagaccggg gactccgccg ggtctccgag cccgtctcgc gagtgaccct
    74281 ccccatcgtc ctcgtcctct ttcctcgttt ccgtccagtc gcttttcggc cggccgctcg
    74341 ccgaagccac ccgccgaaga aaggaacgcc ctcatgagcc ggtccgacca ggccctgaca
    74401 gagacacaac tgaactcgct ctgggagccg aaagacggcc attcgatcga tctcgacctc
    74461 gaggccggcc tcaagctggc gatcctctcc gatctccacc tcggcgacgg cacggcctcc
    74521 gatgacttcc gcgacaacga gcctgcactc cgggctgccc ttcgccgcta cagggacgac
    74581 ggcgtgcgcc tcgttctcct cggcgacatc gaggacttct ggaagttcga cctcgacccc
    74641 gtcgagcgac ggtaccggga taccgtctac aaagaccttc gcgcctttcc gacgccggcc
    74701 ctgcgcatct tcggcaatca cgacatcgag tggcgccagc tccgcgatcc cgcggcggcc
    74761 ctcccggacc cgacccgcga cggattcccc gagggcgttc gcctcatcgt cgaccgcgtg
    74821 cctcgggctc tcctcgtcca cggccatcag ggcactctcg attccgaccg cggtgcctgg
    74881 ttctcgaggt tcttcgtcca catctgggga tcgctcgagc ccttcgtcca cgcactcggc
    74941 cttcatccga cgaaccccgc cgccaccgcc tcggacgtcc tgggcgactt cgagcggacg
    75001 ctctactcgt gggggaagac gcacgggcga gtcctcatct gcgggcattc tcatcgagcc
    75061 tacttcgcgt cgaggtcccg ggcggaacag ctccgtgacg agcaggatcg tctctcccgc
    75121 tcgccggccc ccgctcgatc ctcggcccgg aagtcctcca ccctgaagca gctcgagggt
    75181 ctgcgcgccg agctggagga cgaacggaag aaggggcgag acatcggacc cctcgacgcc
    75241 ggcggcgccc cgggagcctg gtactacaac accggctgcg gtctctacag caacggagtg
    75301 accgcgatcg agatcgagag acctgcaggc gccgcgtcgc agccggtcat ccgcctcgtc
    75361 ttctgggacc gccagggttc gaagacgacg tggaacaccg gccccctttg agcggcagtg
    75421 gcgccgaccg tctcgccgac ggaacgacgt cgacgacacc tgctgccacg cggatcacct
    75481 cgcggcgttc gagccgtcgc cgattccgtg aacgcggctc cgggctccag cgctcaagtc
    75541 tcgcccggtc catagccgca gaagggggcg tcccctgagc atcaatgcca tggcgaacgc
    75601 cgcggccgca cgccgcccgg acacagtccc ggccaacgac ttcccgagga ccctggacca
    75661 gatcgccagc gcagccgcga cgccaccagc gcctgccgag ccgtcgcccg gcccgggcag
    75721 gctcgcggtc ggctcctccg ggtcgacgga cacggcgctc cacgtcctct tcgggtacat
    75781 ccctaccgaa gtcctcacac tctacgtcgc ggttctcgcc gccctccatc gcgagtccga
    75841 agtcacgcgc gccgactggc tcgcgttctg gatcttcctg gccgcgacgc cggtcatcgt
    75901 gtggctcgtc tacggggcga agctgaaggc tcttcagaag ccccttcccc tccgcatcgg
    75961 cacctggccg gtgtgggaga tgttcgcggc tacggccgcg tactcggcct gggctttcgc
    76021 gcttccgaac tccccgttca gtacctaccc ctggtactcg tcggcgctgt cgggcctcgc
    76081 cgtcctgatc gtctcgaccc tgctcgggct catagcgccg ttccttcagc ggccgctggc
    76141 cacctgaccg ccggccttcc gcccttccca acgccgccgg aacggaggtc tgcatgccca
    76201 cataccgcgt cctctccctg gacggaggcg ggatccgggg tctcgtcacg accgtcctcc
    76261 tcgaacggct cgcggcgacg cccggtctgg agaacctgct cgacaagacc gacctggttt
    76321 cgggcacgtc gacgggcggg ctcctcgcgc tcgcgatcgc gaacggcctc agcctcgccg
    76381 agatgcgaga catgtacgtg aagaagggcc cgcagatctt cgacgactcc tggctcgacg
    76441 acctggtcga cctcggccag ttgcggggag cggactacga caccgggccg ctccgccgcg
    76501 agctgcggag gctgttcggc gacacgacgc tcgggaagct ccggaagaag gtcctcgtga
    76561 ccgccttcga cctcgacaac gaagatcccg cccaccgaac gtggaagccg aagctcttcc
    76621 acaacttcca gggcaagggg tccgacggcg cccttccggc gtggaaggtc gggctctaca
    76681 cgagcgccgc gccaacctac ttcccttccg tggacggctt cgtggatggc ggggtctacg
    76741 ccgggaaccc ctccatgtgc gccctcgcgc agacccagga cgcacgctac tcgccgacac
    76801 cgccgatgaa ccaggtccgg ctcctctccc tcgggacggg caccaatctc cagtacgtcg
    76861 ccggcacgac cctcgactgg ggatacgttc agtgggcccg accgctcatc agcctcatgc
    76921 tcgacggaac cgccgggatc gccgactacc agtgcagcca gctcctcggc gaccggtacc
    76981 accgcctcgc gcccacgttc ccgaacgacg tcaacatccc gctggacgac gtgaagaaga
    77041 tcccctacat gctcgacttc gccgcgaagg tcccgctcga cgtcacgatc gactggctga
    77101 agcggttctg gtagtgcccg aaccgcgggc ggctcgaagg cgcctcaacc agcaagagat
    77161 cgagactttc cgtccccgcc ggcagccccg cacaggtctt ccccggaggc cccttccgaa
    77221 acgagcgacc ccccgcctcc gacccgtccc ggaggcatca gcctcgtccc gttcctcgag
    77281 aacacatccc cgagggcagc ttccgctagc cagtcgcaac aggcagagca tgtcgcccga
    77341 gcgctcaccg gtacggaggc gatcgcaccg agtcccctga cgtggcgcgg atcctcgaaa
    77401 cccgcctcgg gcctcctcgg cgctgacacc acccgtccgg gccaccatcg aacttcctcg
    77461 aggccgacct ccggtcatgc gcgtcgtcag cgggcctcgt gcgctggggg aacgcgctcg
    77521 atccggcatt cacaaacttg tatctcacac tcgtacccgc ctcattcgac ttcgccggga
    77581 ccctgtgcac gatcgtcgtg gcgctccttc gaggtccccc gagggcgccg cccctctcca
    77641 tccccgggtg ctccggcccc tccggtccgc tgcctccctg tccgcctcat ccaatactgc
    77701 gccattgccc ccacaaggag acgtcccatg aagcgactga cactcctctc tctgttcgct
    77761 gcggcgttcg cggtcttctg ctccctgccg agcagagccg aggacacgac aattcgattc
    77821 ctggatggct cggtggccaa gcccggtgac acgtggatcg ccgacatcaa acagaaggac
    77881 gggtccgtac ttgcgtacag gaagaacgac gacgacatcg cgaagctcgt cgccgccctg
    77941 cgatcgattc caaaggtcaa gaaggagatg ctgactacag ccttttgggc tgctcgcgag
    78001 tgcgcaaaga cagcgggcaa tcaatgcagc agtggctcgt gcccctcggg caagtcctgc
    78061 aaacagacgg gcacaacctt cacctactgc agttgtcagt aggcctcccg tcactcgggc
    78121 caagcccgcg gccggctcgc gtcggccgcg ggtcccttct gcggcgcccc ggctgagtca
    78181 acggtctcct cgccgactct cgttccacac acttgactgg gccccttgga ggagctcgac
    78241 gcatgatcaa gcgcaggagg acacccacgg cgactggacg gctttccgga ccactcgcca
    78301 gtgacttcga tcgatccgtc cgcgacctgg cggccgcggt cgatgcgctc gtgccagtcg
    78361 cctttgcgtt ggcgtggtct ccaaccgaga actcaccggt cttcatccag gtggtcgccg
    78421 tgaacggcaa cccacggacg cctccttccg gtgaggtcga ccccgcaagc ccctatcttc
    78481 tcgcgctcgg cgcgttcccg gatggcgcgg aaatcaccat ctcctgggtg atacgggcct
    78541 tcgcggacct gcacggcatc cgctcgtttg cgcggaccgg aagccgcccc tgggaacggc
    78601 tagatgacaa ggtgccactc cagtaccagg gacagtggaa gggcgagtcg cgccttcggg
    78661 tgacaccgtg aggcacacgc gtctcctctg cgtcatcgcg tgcctggcct tcccggcaac
    78721 atccaccgca caaccggtcg acggccccgt attcgaacct ccgacctcga ggacggattc
    78781 ggaggactgg ccgcccgctc agtcaaggct cccggctgac gcagggccgg gcttcctccc
    78841 ggctactcca gacctcctcg cagcacactt ctccctgaag cgagagggga ctgcgatcgc
    78901 catcaccagc catcccttca gggtcggctc gcagagggcg ctctggggta tccggacgac
    78961 aggcggctac gacagtggag agaagacgac tgggttcggt cttggctatc gtttcgaatg
    79021 gcgctggctc ttcgctgcgc agtggacgag taggaccgtc gacgctcatc ggcgcgcggc
    79081 ggagatgacc agctcggtat ccgacgagaa gcgagcggcc gaagtcgacc gcgcgatgct
    79141 ccgcatcgcc gatctcgaga cactcagcct catccccata ctcaccgtcg gctaccagat
    79201 cgacctgttc cctgcacggc tcggcggcac cgacgcgagc agcatggccc gcggactggc
    79261 atccctgaac ctctcctggc gatatggtgt ctacgtcgac gtcgaatcga atctgtcgat
    79321 cggccaccaa cgggctgacg agaccagccc tctcgtcggg ttgctctcag cgggcgccgt
    79381 gctcctcgtg acgattccgg agctgcttgg cgccaccgca tacgacgatt actacagaag
    79441 gaacctcctg cagcgtggcg tctcggttgg ccccgcattc tcgtaccagg aatgccgcgc
    79501 ggatgccgcg gagcaggccg catgcccgaa gggcctgatc cgagacagac tcgtcggggc
    79561 ggcgatcgag ctccgcgttg cagacaaggt gacgcctcgg ttcactgtgg ggaagagacg
    79621 tttatggacg agaaagtcgg acacatccac tggtgaggac ttcacggaag gaagcgtctc
    79681 cctcacctac acctttcggt aagcggcatc tagcgctgtc gcgccgtcga tcactcctgc
    79741 cgaatcgcgc tcggctctcg cccgctcctc cggtcgcttc tccgaccggg cgtcggacaa
    79801 gcgcgattgc cttggtcttt cattcaggtg ctcgtgtgcc gatctggcag gtaccgcgac
    79861 accgttagcc cacggcgttc ggcctactcc tcctcgaaac cgaagctctc gaacttcagc
    79921 gtccggccgt tctcggtcac cgtcctcacg agctgctcgg aggcgccgcc aggaatcgtg
    79981 gcttcgatct cgagcgtcac ggtgacggtg gcgccttgca ggccggcgag atgggcgatg
    80041 acctcgtcgg cgatccggct cgcgtccctt ccggcgcgag ccggatcgag cctcaccgtg
    80101 ccgtgatagc ggcgcggggg gacgggcccc gtgggaagag tcgccggcgc ggggctggca
    80161 ccgggagcag aagacgaggc ttggccggcc acggcgcttc cggcagtacc tgacgctgag
    80221 gccgaacccg cccccgtcgc ggcggcggcc gccgccgcgg cctgcgtctc ctcgtcgatc
    80281 tccttccgcg cgacgtccgc cttcacgaga agtcccgtgc tctcggccgg cacggcgacg
    80341 acctgcccgc actccagccc ccggtagcgg gacgccgcct cgtcgaagct ctccgcgtac
    80401 gcgaaggcgg cggcctgctt ctgcgtctcg gcctgccgca cctggcgcgg gtcgaggttg
    80461 agggtgttcg tgtccgcgac gatggactcc caggcgagga acttccggag cgcctcgtcg
    80521 aggtcctcga ggcggacacg gtcggccgcg agaaagacga gagtgttctg gtagaggcgc
    80581 ggcgcgctgc cgcgtgacgc caggatcgcc ttcgccgcga cctcggccgg gctcgaaggc
    80641 tccttgccgt aggggtgctc ggccgagagg acgacgaggc gcgtctcgag gtcgtccggt
    80701 acgtcggcgc cggaacgcgg gagcgggtgg atgcgcgaga agtcgcccgt cttccggagg
    80761 tcggcccgaa gtcggtcctc cagctcctgc gcgaccttgt cggggtcgcg cttcagctgc
    80821 tcggcgcggt cctcggcgag cttcgtcacg gtgggctgcg ccgcgtacct ggcgttgcgc
    80881 accgcgcttc agagccacag gggcggcgcc ttccgcctga tccttctcgg cgacatcgag
    80941 gacttctgga agttcgatct cgatccagtg gcctggcggc atagcgactc catttgcgcc
    81001 gatctgcgcc catccgcgcc ccggccgccg cgcgcccttc ggcaaccacg acatcgaatg
    81061 tgtcgcctga ccgaccaggc tgggcacccc ccgcgccgag cgccgacggc ttccctgagg
    81121 ccgtcaaact gatcgtcgac aatgcagcca aagccatcct cgtttacggc catcaggaaa
    81181 cggtcgattc tgaccgcgga gcgcggttct cgaggttctt cgttcacatc cgaggatcgc
    81241 tcgaacctct ccttcacgct ctcggcctgc tccctgccaa tacggacgcc accgcgtcgg
    81301 aggtcctggg cgacctcgag cgaacgctct acctctggat cgggacactc ggtcgttcgg
    81361 tcgcccacgg gcactcttat cgggcgttcg tcgcatttca gtctcgcgct gagtgccccc
    81421 gcgacgaaca agaccgcccc tctcgatcga tcgccgccga acggtcggtc gccgtgaacg
    81481 ccacgaacgg aatcgcactc gaggacctac gagtcgagct ggagaacgag ccgaagaggg
    81541 gccgggacat cggcccgctg aacgccagcg gcgctcctga agcctcgtac caggacgctg
    81601 gctgtgacct ctacagcggc agcaccacag ccctggagat cgggcgtccc caagatacct
    81661 cggccccgcc ggtctttcgg ctcgtcttct gggaccggcg gggtctgagg gcgacctcga
    81721 ggacgggtca gctgtagctc cccgccagcg gcaggtcctt ctcggaacca gacactctcc
    81781 gtcggcggtc gttgggcggt ccttcctccc gcgagacggg ctctcgcgcg caggccctca
    81841 tgacaacgtc gcgctgcccc gcacggtttc cgcgcacccc accaacgggt actgcagcga
    81901 cgctcccgtc aaccggcggg gaccggttcg tccggcagcc cgccgaagcg agtcgcaggc
    81961 gcccgtaggg cactggcgac gagacgcgtc gccttcgcat ctctccctcc cgtggcgcgt
    82021 acggcgcccc gagcccgcga ccggccggat aaccaaggcc cttcatgcga cacgttcacg
    82081 atcctggcgg ttctcctcga ggacccccgt gtctacgcga ctcacctcgc gccggatccc
    82141 ctcttccggt cactcctccc ggacctccgc ctgccgctac gcccatcgac gcgtaccgcg
    82201 tggtgccgtc gcagacgtcc agacctccgg gccaggcgct ttcgacacga gcattcccgc
    82261 acctcgtttc aggcccgcgc gatccggctc gaccgggccg tcgcgagctt ctgcagtcct
    82321 caccaggaga tcccatggca aagcccccag cacccgccga cggcgggaga tcatccggac
    82381 atcaaacgac gaagccctat tgtcggaacc tcccgcggag cgctcccgcc ctcccgccag
    82441 ccgtcaccgc ggaccctgcg cgcgcggcgg caatagtcct cggacggtcg aagtgggtca
    82501 acggaaccgt cctgcactac gccttcttct caggaagctc tcgctactcc gtgccacatg
    82561 gtcaagccga cgcagtgcga gacgcattcg cgaagtggaa agccatcggc atcggcctgg
    82621 aattcaagga agttccgctg ctcgcgcagg ccgaggttcg ggtgggatac tccctcgtcg
    82681 acggaacgtc cgcgtccggg gtcggtcgcg acatcctcaa catctcgctc aacgagccga
    82741 cgaccgtcta cggatgggac ctaacctcgc catacggtcg cggcaccgca cttcacgagc
    82801 tcggccacgt cctcggaatg gagcacgaac accagaaccc ctatgccggc atcaagtggc
    82861 atgagcaggc ggtctacgat gctcttgcga aaccaccaaa tcgctgggac aggtccacga
    82921 cgtatcacaa catcctggag aagctttccc cgcagcaggt ccagggctcg cgatgggatc
    82981 ccgactcgat catggagtac gagttcgagc ccggcctcat cgacgagcct caggaatacg
    83041 acgtgaacgg gctcacgccg cccggcacac tgtccgctga cgacatggcg tgggtacgca
    83101 aatggtatcc gccggtagac cacaccctgc cgcttctcgc tccattcgag tcccgcgccg
    83161 ttgacctggc ggcgggcgaa caggtggact tcgcggttgc tcccgccgtc tcccagaagt
    83221 acacaatcga gacgaaaggt gcgacggaca cgctcctcgt tctcttcgag gacgtgaacg
    83281 gcgagcctgt gtatctctcc ggcgacgacg acagcggcga agagcggaac gcgatgatca
    83341 cctacaggct cctcgccgga cggcggtaca tcgtgcgcgt ccgcctctac tatccgggtc
    83401 gatccggcaa gacccatctg atgatcctct agctcgcccc gtcacaccga cgtagactcc
    83461 tcgagggttc ccgtccccat cgttgagccc tcgctgtgag cccgttcgcc ggccacgtga
    83521 ggcaccgact ctccctgtcc ggagatgcgg cgaccgtccc ttcctcttca cctgctccgc
    83581 catccctcgc tgcccccggg ttcggcgcgg ccgacggcgg tgatgccatc gccccatcgt
    83641 cccgcgccgc gtcggagata acgccaatcc aacctgacgc gacactcata cagggaggcg
    83701 tcccatgaaa gtcaaggaac tccggagcat cctcaacgct tccaaagccc agtgggccat
    83761 accggccgac atcgccgacg acaccgagct cgaagccatc tcgagaccgt tcgccatggg
    83821 cggcatgcca cctgcccctg gagcacccac ggctcgatca ccgcgaatgc gccggcccgc
    83881 cgatgcggcc gtcgccctct ggcagcccgg cgggaaccgt ctgacacgac cgttcgtcac
    83941 cgcgcttccc aggacgtggg actggcgcga tgtccatgga cacacttgga tctcgcctcc
    84001 gaagaaccag ggtggctgcg gctcatgcgt cgcctttggc gtctccgccg cgctcgaggc
    84061 tcaccagcgc atcgaggcga acgacgcttc cctacccttc gacacgtccg aagccaatct
    84121 cttcttcgtc aatgagcggc agtgcaatct gggtgatccc agatacggct ggtccatacc
    84181 gcccgcactg gattacctcg tcgaccaggg cgcctgtccg gaagaggact atccctaccg
    84241 cgacgtcaat cagaatgccg agctggtcct gggcacgcag ggcacgtgga agatcaaggg
    84301 ctacgactcc acgagcaacg tcaggcaggc gaagcgatgg ctgtgcgagg acggccccct
    84361 cgtcacgaga ttcaccgtgt atcaggactt catgacgtac tggagcctcg gtgcaaacgg
    84421 cgtctacacg catcacgctg gaagcacgct tggcggacac gccgtcgcag tgatcggcta
    84481 cagcgacgag caatcctgct ggatctgcaa gaacagctgg ggctcgaccc acggcaacga
    84541 cggctgcttc cggatcggat acggccagtg cggaatcgac gatcgaatgt acttgatcca
    84601 ggatgtgtac gacgtcctca cacgagacga gatcgcatac aacccgcgca cgctgcgaat
    84661 cgtcgacgaa ggcgcaagag gctggttgct gaccgacggc gtgagccgaa tgaagattct
    84721 cgacagcaag gaggacgctc gcaacgctct ctgcgtcgct cgcagataca cgcgacaggg
    84781 cttcgtgggg cgggacaacc gcagagcgaa tagaatcgac tacattacgg aatactgggc
    84841 cgggaacagc ggtctcccgc acgagccgct gacgcgagag gatgcgattc catacgatcc
    84901 ctcgaaggtc gtcgccgagg acatcgatgc cgacggttgg cggctcagag agggaagcca
    84961 ctggatgctg ctggcagacg atctcaacga tgcattggcc gttcttcaga tcgtcgagcg
    85021 ctacacgaag atgtgcttca tcgggagggg caatcatcgc cccgacagga agtcctacat
    85081 catgacctat tgggagtgag ccgctcggcc ttctacgacc aggctggcca tcgcacggtg
    85141 agcgacctgc gacggccacc gcgtcgcgcc cgaatgagcc cggcgcggcc ggaacacgct
    85201 gcaggcggca cgcaccgcgg gagcggatgc cagccccgcc caggtctcgc gagcgcctcg
    85261 ccgtccggcg aagcgacctc gcgagatgcg tgtcgtcccg cctactcctc ctcgaagccg
    85321 aagctctcga acttcagcgt tcggccgttc tcggtcaccg tcctcacgag ctgctcggaa
    85381 gcgccgtccg gaatcgtcgc ttcgatctcg agcgtcacgg tcacgtccgc gccttcgagg
    85441 ccggcgaagt gggcgatgac ctcgtcggcg atccggctcg cgtcccttcc ggcgcgagcc
    85501 ggatcgagcc tcacggtgcc gtgatagcgg cgcggcggaa tcggacccgc gggcaccgtt
    85561 gccggtacgg ggctcgcacc gggagccgaa gacgaagccc ggccggagac tcctcctgcc
    85621 accgcacccg gcgctgatgc agagcccgcc cccgccgcgg cggcggccgc cgcggcctgc
    85681 gtctcctcgt cgatctgctt ccgcgccaca tcggccttca cgagaagccc cgtgctctcc
    85741 ggcgacacgg cgacgacctg cccgcacttg agcccgcgat accgcggccc ggcctcgtca
    85801 aagctctccg cgtacgcgaa tgtatcgacc tgccaggtca ggaggcgcac gccgtcggtg
    85861 acggcgtgcg ccaggacctc cgggccgagg agccgcggaa ggtagaggta ccgcgcgaag
    85921 tcgtcgacga gctggcggat ggcgacgcga tcgcctcgcc agaggggaac gtcgtcgagg
    85981 tgcttgcgaa ggaccgtcga gccgagggtc gtgacgagcc actcgtcgct cttgagcttc
    86041 ttgctcgcac ggacggcgag ggcgtcggtg ccggtgaggc gtgtggcctc ccacttcacg
    86101 ggcgcctggg ggttggcctg gcctggcgcg aggagccact ggtaggtctc gggaaggcgc
    86161 gcggtcacgg caccgtcggc ggcctgcttc tgcgtctcgg cctgccgtac ctggtgcggg
    86221 tcgaggttca gggtgttcgt gtccgcgacg atggactccc aggcgaggaa cttccggaga
    86281 gcctcgtcga ggtcctcgaa acggaccctg tccgccgcga ggaagacgag ggtgttctgg
    86341 tagaggcgcg gcgcgctgcc gcgtgacgcg aggatcgcct tcgccgcgac ctcggccgga
    86401 ctcgaaggct ccttgccgta ggggtcctcg gccgagagga cgacgaggcg cgtctcgagg
    86461 tcgtccggca cgtcggcgcc cgaccgcggg agcgggtgga tgcgcgtgaa gtcgcccgtc
    86521 ttccggaggt cggcgcggag tcgatcctcc agctcctgcg cgaccttgtc ggggtcgcgc
    86581 ttcagctgct cggcgcggtc ctcggcgagc ttcgtcacgg tgggctgcgt cgaataccag
    86641 gcgcgcgggc cgtcctggta gaggtacgtc gccgccgcgg cgagccgcct caatgcgtca
    86701 ccgaagacgg ccggaggctc gcccggcatg acgcagccga gcttcacccg ccggtcctcg
    86761 aggccgcgat gcgcggcgga ggtcgtcggg gccgagccga ggtagatcgt ccgcgccacg
    86821 cggcgggtcg cgtggagctt gccgaggttc gggacgtcgc cgtcgagctt cagcgggagc
    86881 gaccccgggc cgtccacgtc cttctcgagg atggggaccc agttgtcgga gaggtagcgg
    86941 gtcagctcgt cccgcacgcg cccgtcgtcg atcgggatca tcgacggcag gatgagcggg
    87001 ttcttgtccc ccttctccca gaggctgtgg atgacggctg ccatcagccg caacacgccg
    87061 cgcgtccgct ggaacttcgc gagcgtcgac cagtcggcgt agagccggtc gaagatctcg
    87121 gggtggatcg ggtaggccgc ctggatccgc ttctcgtagt cggccgtccg gcactccggc
    87181 gggaactccg cgctctgggc gcggtaggtg tcgccgaagg cgcgcgccgt caggtcgcgc
    87241 tgcgtgaacg aggcggggcc ttcgagcggc tcgaagaggc gccgcctcac gatctcgaac
    87301 ccctcgtccg acgtcgccgg ccgccacgac gcctcgagcc ggcccacgac gttccgcagc
    87361 cggtccagcg cctcgcgccc gcggatccct cccacctcaa cgtcgtcggt ctgcgtgtgg
    87421 ggcgagccgg tcgtgtcgga ggcggggagc gagatgacga ggaggcagtt cttcgcgagc
    87481 ttcgccgact cggtgaggac ctgcgcgaac gagaactgcg tctcgaacgt ccccgccggg
    87541 aggtcgcgct ggtcgtgcag ctgccgggcg taggcgaccc actcgtcgat caggacgagg
    87601 cacgggccgt actccttgaa cagctcgcgg agcgcgtctc ccgggctcgt cgccttctcg
    87661 tcgtccgcct tcaccttcgc gaaggccttc ttcccgccca gctgccaggc gagctcgccc
    87721 cagagcgtcc tcacgaccgt gccgtcgggc ttcgtgaccg ggttgccggg cgagatcttg
    87781 ttccccacga ggacgacgcg cttcgccgcc gggaccccct tgacccccgc ctcggcgagg
    87841 atcgcctcga cccccggcag gctcttggcc gagacgccgg agaagaggtg gtagagcgcg
    87901 agcatcgagt gcgtcttgcc gccgccgaag ttcgtctgga gctggacgac cgggtcgccc
    87961 cccttgccgt agatccgccg gatgccgttc acgaggagcg cccggaggct ctcggtgagg
    88021 tacgtcctgc ggaagaactc cgtcgggtcg cggtactccg gcgtcccctc gccgaggtgc
    88081 acttgccaga ggtcggccgc gaactcggcc tgctggtagc cgccgctcgc cacgtcggcg
    88141 tgcggcgtca cgacttcgcg ccacggcttc agagtcccgc ccgcggcgac gtcgatgagc
    88201 gaggcccccg ccttccgctt ctcgctccgc acctgctcgt cgtagacgag gcggcgcagc
    88261 tcgagcttca tcttccctac ttcgtcggcc tgtggcgccg agatcgccgt caggaggcgg
    88321 gccatcgagt cgagcgcgcg gtcggcgtcg tcgctcgaga gcgccttctg gtgcgcccag
    88381 tcgttccgcc agtcgcccag ctcctggacg agcgtccgct cggcgcggcc gaggacccgg
    88441 ccgaagacgt cgttccacgc cccgcgcatc accttgagga gcgccgccac gtcccagtcg
    88501 gcgagcggcc gtttcgcgac gttcggctcc tcgcccacga gcttcagcgc ctccgcgcgc
    88561 gcccggtcgc cgtgcgcgtt cgtcagctcc cgctcgacga acggggccag cccctgcttg
    88621 aggaggtcca tcgccttccc gacgcgttcc tggttggtca gtgccatgtc cttggttcct
    88681 tcttcagtcc ggagcggaga gcatccactc gtaggccggc tggacctcga cccctcgcgg
    88741 gacctccgcc ggtctgccgt cgctcgtcag ggtgaggagc cgcttcttcg ccttcggatg
    88801 gatccggccg gcctccacga gcgctcgcag ctcccgttcc gccgtcgccg gatcggtcgc
    88861 gtcggcgcag acctggatca ggtcggcctg gccacccgca ccgcgcgcga ggaagtcgac
    88921 ctcgtgcccc tcgggcgtct tcacatacgt cacggcgagc tgccgccgct gaagctccac
    88981 gaggacggcc gtctcgagcg catgccccgt gttcgcgcga cccgtccggt cgaagagggc
    89041 gatgagcgcc gtgtcgaccg ggtagcactt ccgcgggttc accatccgct gccgctccga
    89101 ggccgactcc atccagacgg gcctcacgag gaagcagtcc tcgagccagg cgaggagctg
    89161 atggacggtg tcccgcgcga tggcgacccc ctggctcttc agcgcggcgt ggaacttctc
    89221 cacgctgaag agcgcccccg cgttgccgag gagctgcctc accatccagc gaagcccgac
    89281 gacgttcccg accccgtggc gctccatcac gtcgcgcagc atcgcgacgt cgacgtagtc
    89341 gctcagcatc ttttcccggg aagcggcgtc gagcttctgc acctccggga agccgcccgt
    89401 cgagagccag tcgaggaacg cccgctcgat gcgcccgcgt tcggcgccgc cgaggaagcc
    89461 gggtctcgcg ggaatcggcc gcccctgaaa gaggagcgcc tcggcgaagc tgaacgggta
    89521 gatcggcacc tcccaggcgc ggcccctgag cgccgtcgcg atctcgcgcg aaaggagcgc
    89581 cgccgaggac cccgtgacga ggatgtcgtt tcgcccttcg tcgaggagcc gcctcacgaa
    89641 ccgttcccac cccttcacga gctggatctc gtcgaaggac cagaggacgc gcgcccgttc
    89701 gcgcgcctcc gggaagagcc ggccgtactc ctcgaggagg aggccgagct gcgccgccgt
    89761 catctccgac aggcgctcgt cctcgaagtt cacgtacggg acgtgctccc gcgccgcccc
    89821 ggccgcgagc tgctcgcgcc tctcctggtg gacgaacgtc gtcttcccgg accggcggac
    89881 gccgacgacg gagctgacct tccccggcag gctcgcggcg ccgtggagcc tgcgcggcgt
    89941 cccctccggg atcggctccg tcagcgactc cttcagcttc gcgaggagga cgctcttgag
    90001 gtccgaagcg gagagctcgt tcatgcccgg catctctcct tcctacaagg agagtttaga
    90061 gcgtgtttcg ccgtctcagg aggagaaaag gtcggcctgg ccggcctccc tcgctccgcc
    90121 ctcccgcgcc agccggacga tctcgggcca gctctggacg agagcgttgt aggagagggc
    90181 ctcgggggcc cgcttcttcc gctcgcagat cgtgtaaagg cggtacgcga gctcgcgcgc
    90241 gacctcggcc ttcgccccga gcttcgccgc gagcgccgcc gccgccgcct cgccctcggc
    90301 ctcgaggacg cggatcaggt ggtggaccat ctcccactcg gtcagccgcg tgtcgctctc
    90361 cgggtcccat ttcggcgaca gctcggccgg cttcaggagc cgtaccttgc cgcggctcga
    90421 cttcaggatc cccgcgtcga cgagtcccgc gacactcgtg ttcttcgcct tcgagagcgt
    90481 ctcgccgacg ccgaactcgc cttctccgaa gccgtgctgc tcgaaccagg cgagcgccca
    90541 ccggctgtcg gcgtcgaagt cgccctcctg ctccgcgagc gcctcgtcga ggacctggtt
    90601 gatgagggcg agcgcctcgc ggacgctgac cggcttcccc tccgcgtcga ggaccttcga
    90661 atagcgcgtg tagaccgcca tccccggccc gatcgccgcc tgcgcgaggt cgacgggtgc
    90721 gatgttcccg cgctggaggt gggcgagggc ggcggggagc tcggccttga gggcgttcac
    90781 gagctcgcga cgggtggcgg tgggcgcatc ggcggggcgg gggcggcaga cgaggacgat
    90841 ggaggaggcg agggcgttgg aggccacttc gacggagcgc ccctgccgct cggtccgcat
    90901 gggccaagta ccgctcacgc ccaatccggc cctaatgacc gcgtcgagaa acgtctccca
    90961 gccggtgctc gaagtcccga gatcatccgg gctttccgcc tgtttgaagg cgtagtagat
    91021 cgtgacggga aaggccgagt gcgcctgcgc agaaagacgg gacatggcag acgtcatgcc
    91081 gtcaaggaag aaggcctcgg cctgctccac cccgccatgg cggtacgggg cggccaccag
    91141 ctctccaatc tttggcactg caagcgtcct gaagagctcc gggaagaccc tctgcagagt
    91201 ccttcgaagc cagacataga agaaatccga cagctccgca tatccaatgt tgttgtagta
    91261 cggaggatct gtggacacga ccctgccgtc cgcggcgacc tccgtcgcgc agtccgcctg
    91321 aaaggccgtc cccggcttcg tgccctcaga gaccgagagg gccgctacgc tctcggctgt
    91381 ccacgcggtc gactcggaca aagacctcgt gcagtctgcc agcagatcta cttcagcaaa
    91441 atcccaagtc atcggaaggg cttgccggct aaacgtgcta aagagggagt ctcgctgcgt
    91501 catccatcgg cagatcgtcg aactcccatc gatcgacttg ctgagaacga aagagaggta
    91561 gctcgcgacg gcttccggat agcctatggg ccctccctcg ccttccctct ggtgtccatt
    91621 gcagtacggc ggccccgccg tcacagaatc ccgctcgacg cgttccttcg cgtcctgcac
    91681 aaggttggag aaggtggtca gtgccacaag ctggcgcggc gtgaagagat cgccccatac
    91741 cgagagaccg tatggcacac acgttccacc ggtaagcctc gcgggcacat cgccctccgg
    91801 tctccagtct ggcttggcct tccgcgcaat cgcctcgtgc tccgacgtcg gcgggagata
    91861 caccctgctc cgttccccct ccgcgacgat cgccatcatc cgggcaccca tccggccggc
    91921 cttcccctcc gccttgatgt agtcgccttc gatcgccgtc cccgacatta agcaccgaaa
    91981 gttcgccccc cgcgccagct tcgtcccgtt cttggcctcc tccgccttct tcggcttccc
    92041 caccttcacc gtgaaccggt agccgccatc ctcgatgacg ggctcgacgt aggcctcctt
    92101 cccctccttc gtcgagagca tgaaggtcga ggcgagcggc acgtccacct tccggaaggc
    92161 cgggttcggg cttttcaccg tccgcgccca gagccaggcg atgacggtca gcttcttgcc
    92221 gacgtacggc ttcagatcgg gccgcccctt cgccatctcc gccgtcacct cgaccttcgg
    92281 gtagaggtgg ccgatccgct tctcggcctc gtcgcgcatc cacttgccgt agtagcgaac
    92341 gtcctcggcg agcccctgcg cccccttcca ctcgcgggcg atcagctccc cgttcgcgcg
    92401 cgcctcgggg ttcacgggcg gccgccccgc gaacttcggc gggatctcga tcatcgcctt
    92461 gttgatgagg acggcgaccg ggttcaggtc gctcgcgtac gcctcgaggc cgagccgctg
    92521 cgcctcgagc gggagcgagc cgcccccggc gaacgggtcg tggaaagcgg gcagcttgtg
    92581 ccggtcgaag agctccttcg cccgcgggtg gtccgcgttg tcggcgcagg cccgccgcca
    92641 gctctgccag atctctgccc gcgccgcctc gaggacctcc tcgttcgtcg tgttctccca
    92701 cttcacgagc tcttcgatga tcttgaagag ccgctgccgc tccttctcct gcttcttctc
    92761 cgtcgggaag agatcgggac acgacgacgg gtcgtcgacc atctgcgcga agatgaccgc
    92821 ccgcgccgcc gcgagcggcc gccgcgccca ccaaaggtga agcgtcgacg ggtgcccgtg
    92881 ccggatcgac ttctcccgcg ccgacgcctc gttgatcgcc tgcagcggca gcgccacctc
    92941 gatcagcttc ttccggctga tttccctgct catgctctcc tcgacctcat cggctctcga
    93001 gctctcccga ctcccgaagg cgcacgcgcc ggccgctcag tcgtccgggt tcgccggatc
    93061 cctgagagga agtccctccg gcaagaccat gtcctcggcg tagcggacga gcgcttcggc
    93121 cgcccctcgt ggcgatcgtg acgggaggag cgcttcggct cgcaagacgt ttcgcacgat
    93181 ctcgtcctcc cggcagagcg ccggaaacag ctcgtggtag acgaagcggt tccacgcgtc
    93241 gagacgcgcc ttcccctcgt cgccgaagag ctccccctgc gcgtcatacc gttcgcttcg
    93301 ataccgatcg cagacggcgt tccagaacgg gaggagaggc cccaggtcga ggccgccctc
    93361 acggcgggcg tgctcgagca cggcggcagg ctcaccgcga atctgaagca gccgaagcgc
    93421 gcccaggtac cggccaatgc agctgcgcac gagactggac agggccgggc tcgtccattc
    93481 ctgctgcctt cgagcgaact cctcgcgtgt catcccagcc tccgagtcag cacgtctccg
    93541 tccgagatac gagcacggtc cctattcgat ctcggaagcg gcgaaacgat cgattccctg
    93601 cggcctcgtc gaggtcgaag tgcttagtga aggcgaactg atgcgtgaga gggtcgtacc
    93661 ctccggccat ccggtcaccg agccagccct tcgcgtcacg aatcgactcg tgctgcggcg
    93721 ggcgatcgag gtccgacggc aggccgtgcc ggtcgcgcag ggaccgggcc gccgccaaga
    93781 accagctctc gtactcgcgt tgggcgagaa cgatgagcca ttcgacgtcg gatcggacgg
    93841 ccctgactct tcgaaggaga tccgggccga gcgtggccgg gcagccgtcg tcgcagtcga
    93901 ggaagacgag gacgaggccg cccgcctgcg ccgctttctc ggctgcgaga gtggcgtggc
    93961 gcttaaggta ggccccgtcg tgcagaaagg aaccggcttt cacgcgtatt gggtgattga
    94021 ctcgcactga gcgctcgatt ccagcggcgc tcgcgagcct gtagaggaga gctggcacgg
    94081 cttccttctc gccgtgcccc tccacgatcg gagcgatgta gatcggcttc atcgtgattg
    94141 aagctcgaag agctcgagct gacgctcgtc gtccacatcc tggagagctg tctcgtcggg
    94201 cgcgagctgg ttctggcgaa gcagctctcc ggcggtgaac agcttctccc gaagcacgtt
    94261 acggccggcc gcgtcgatag gccctatgcg tgtaagcccg tcccggttgt cgacggcgcg
    94321 gagggcgtcg atcggaatat ccgggttgtc gaggaggtcc gggctgtggc tcgtcacgag
    94381 gatctggcag gttcgcgaag cctcgcggag ggctcccaga agaacgccag ccgctgccgg
    94441 atgaagcgcg acctccggct cctccacgcc cacgaccagc ggaggtatag ccccttcgcg
    94501 ccgcgacgcc gcctggaacg cggcgagcag gactccgaaa gctcggagcg tcccgtcgga
    94561 catagaagac gcgagaaacc gccaggggtg cttctggccc cgcaccgtct gacggaactc
    94621 gatcgtctcc tgcgaagcga gcgtcttcgg atcggcagac gagagcccgg ctacgatgcg
    94681 ggagaggtgc tcgtccaccc gctcgcgcac cgccggcgtc agccgctgca agacgctcgc
    94741 cgcgttgctg ccatcccgcc ggagaaggtc tcccggatcg ggcttctgca tggcggcaag
    94801 ctcacggggg atgaggttgt agatgcgtat ccgcgagagt gcgtcgaaga ccggtcggaa
    94861 ctgcggcgtc cccgacgccg ccacgaggaa cagtcgatcc ggaatcgccg ccggctgcgg
    94921 ggagaaggac gcggaagtca cctttccccg gtcgaccctg aagtgatctc cgccctcgag
    94981 cgccgcacct tcggcgaccc gacattcctc cgtctggact tcgtagccgc ccgcggatcg
    95041 ggcggcgacg cgaaagctgt agtgaccgac cctcccgttc gggagcgaga aatcgaggcg
    95101 tatcgcgaag tggttcggat gtccccccga ccgcctccgg acctccttga tggtcccccg
    95161 gtcgcgcaac gcgtggtcca gggaggtcga cagcgactcg gagacgagcc ggagggcatc
    95221 gagaaagttg ctctttcctg ctccgttcgg gcccacgagg aagacgagcg gtccgaggtc
    95281 gaccctgcac gcggagatgc tcttgtagtt ccgaagggtt accctgcgaa gtagcggacc
    95341 ggtcaggttc atgttccctc cggtgatctc cgaaacccgg gctcgacgtc tggaaatgca
    95401 tgtcgtgctc gagccccggc gaggtgcaag gcgggcatca gatcaccctc caccagggcg
    95461 ccacgaggac gcgtttcgtg accttgtagg tctccctccc gccgtagagg aggaggcccc
    95521 cgtcggcgag gtcggggtac tcgtcgagaa acgcctcgag cccggcgacg tcggagcgcg
    95581 tgacgcgcga tgaagccttc acctcgatcg ggaggaggcg gcgcggcgtc tcgacgacga
    95641 agtcgacctc ctgcccgctc gccgtcctcc agtagaggac ttccggccgg ccgaccgtga
    95701 ggtcgcgcca ggcgaggagg tcgacgagga cgaggttctc gaggtgggcg ccgcggggct
    95761 cggtctcccc cgcgaggtga agggcgagac cggtgtcgca ccagtagagc ttcggcgcct
    95821 tgatgaggcg cttcgtccgg ttggccgagt aggccgggag gcggacggcc tggtagctca
    95881 cctccaggat gttgaggaag cggtggacct ggggctggga aatcccgatg tccctcccca
    95941 gctcggcctg gttcaggagc gagccaatgc gcaggctcgc ggcgcccatg agacgccgga
    96001 agtcggccag gctctcgacg gcgcggagcg tctggaggtc gcgctcgagg tacgtctgga
    96061 cataaccgcc gtaccagctc gaccgcaggg actcgtcggc gatgcggtgc gccggggccg
    96121 ggaagccgcc gagccgagcc gccgagctcc aggactcctc gggcccgggg tgcgcgtcga
    96181 ggacctgcgg ccagcgcgag aacgaggcgg cgagcagctc gctccagggg ccggtcgcgc
    96241 cctcgccgtg ccgttcccgt cgcgtgaacg gccaaagcgt cacgtagacg gcgcgccccg
    96301 cgagcgactc gctgactttc tccatgagga ggaggttcgc cgagccggtc aggacgaaca
    96361 tcccgggcgt ccgctggtcg tcgaggtcga cggcccgctt cacggcgatg aggagatccg
    96421 gcgaccgctg gacctcgccg agaacgggga ggggcgcacg ctgcacgaac gcctcgccat
    96481 ccgcgtcggc gtgcgtcctc aggtcgatgt cgtcgagcga gaaatagggc cggcgggcga
    96541 gagccgggag cgtctgggcg agggtgcgct ttccggcctg tcgcgccccg aggacgacga
    96601 cgaccggcat gatcttcaga gcccgctcga tcgcggcccc cgccgcgcgc gggaggacgg
    96661 gcggctcgtg tcgcctcacc atcgcagtat gataatcata ccgcaaagga attccacaaa
    96721 ggtcacgccg gcgcctccgc ccgcgcgatg agatcgccga acgcgtagtt cacgctgcac
    96781 gcgccgaagt cgggctcgcg ctggaacggc ttgcgcacgt agtggacgcc gtgcgcgccg
    96841 tcggggagga actcgacgat ggcgaggatg aagtcgtccg gcttgttgag gctgtagagg
    96901 atttcgttct tcgtgacggt gatcgtgtcg gcgccggaga cgcgtccctt gacctcgagg
    96961 aagcggaggc ggccggtgcc ggggacgcgg ctctcgacgt cgtagccgag cttctcggtc
    97021 tcgcggtcga tcgggtcgaa gccgaggccg cgctcgacgg ccatgacgat ctcgcgggcg
    97081 cgggcggcgg aggcctgcgt gtcgggcgag gcagcgaggg cggcggcggg cttcccctgc
    97141 atcgcgacga ggaggcccat cgggacgacg acgaggccgc cgaggaggac cggcgggagg
    97201 gcggaaagct gggcttcgcg atccagctcg gcgaggcgct tctggagccg ggactggagg
    97261 tcgtcggcac ggcggcgcgc ctcctgggag ttgaggcggg cgccgggcct gccggcgtgc
    97321 tcgcgcgcct tcagctcctc ggcgcggtgg tcccaccagg cgatctcctt cgtgaggcgg
    97381 tccttcacgg cggcgcgcgt cttctcgacc caggagagcc tccggtcacg cacctcgccg
    97441 acgtgccccg ggacgaccgt ggcgatggcg tggccgagcg cggtcttctc gaggtcgcgg
    97501 gtgatccagg ccatctcggg gcggccgaag agggcgtcga tcgccggctc gccgggcgcg
    97561 agcgggcgat agtcgaggta gggggcgtac tgaaggtgcc gggcgtcgcc gtcggcgtcg
    97621 agctcgacgt agagcatccg ccgcgagatc gtccgccgct cgcccgaagg gaggaggctc
    97681 gcgtcctgga tggcgtgctc gagcgtgaag aggacgcggg ggcgcgtgcc gggatctcgc
    97741 tcgtcgacga ggacggtgcc gcggcggagg aggtcccgat gccgctcgag cgtcaggtcg
    97801 agagtcgcgt cgaggagcgg gtggccgggg cagaggaagg cggcgagagg ctcgccggcg
    97861 ggggcgatga ggtccttctc gaaggcgatc cgctcgtagc gcgcgaggac gggctcgccc
    97921 atgccgacct gccggtcgcg gttcttcacc ggggccggga cgtgggtgac ctcgtaccgg
    97981 cgcggctccc gctggcggac ggtgccgccg aggcgccgga acgcctcgag gaagaacgac
    98041 tcgacgtagt ggggctggag gcgccgcgcg tcggcccgct ccatctcctc gcggacgcgg
    98101 gcgacgcggc tcgtgtccat ggcgtcgtgg gcgagcgccc gctcgtcgat cagctcttcg
    98161 aggtgcggcc ggtcgatcgt ctgctcgatg gcccgcgtga ggcgcgcgcg cacctcgggc
    98221 tggtcgccgt agcggatcgc ctcgatgagg aggtcgcgga gcggcttgcc gtcgaactgg
    98281 agcttgccga ggacgtcgaa gacctggccg ccgagggcgc tccgggcctc ctcgagcttc
    98341 tcgaggaggg tgcggtagac gtcgccctcg cgcgtctcgt cggccacgag gttccagagg
    98401 tggcagacct cggtctggcc gatgcggtgg atccgcccga accgctgctc gaggcggttg
    98461 gggttccagg ggagatcgta gttgaccatc aggtgggcgc gctggaggtt gatcccctcc
    98521 cccgccgcgt cggtcgcgag aaggacctgg accatcgggt cgtggaggaa ggtctcctgc
    98581 gccttgcgcc tctcgtcgcg tcccatgccg ccatggatga cgacgacggc ctgcggccgg
    98641 ccgaggagcg tgccgatctg ccgcgcgagg tagttgagcg tgtcgcggtg ctcggtgaag
    98701 acgacgagct tctgccgggg tgacgggacg ggcttcggga gcggcccgga gccgtagggc
    98761 gcgctttcct ccgcgacgct ctcgccgaac gccgcgggcg tgaagatctc gccgaggagg
    98821 cgcgagagct cgcgccactt cgtgtcgcgg ccgctccggc ggacctcggc cgcgaggcct
    98881 tcgagccgcg cgagagtggc gatctcggtc ttcagctcga cgatcgtccg cgccgcggtc
    98941 gcctggtcga ggatctcctc ctccgcctcc tcgacctcgt tctccggagc ctcctcgagg
    99001 tcgtcgacgt cgtccgggtc gaggtcgggg ccggcggcga tcaccgcggg gagcgcgccg
    99061 ccgcgctgct ggagctccag ctcgcggagg cgcttctcga gccgctcctt ccggcggcga
    99121 agcgactggt agatcgcctc gggggaagaa gcgaggcgcc gctggaggat ggtcagcgcg
    99181 aagccgaccg tcccggcccg cttgtcgttc gcgagcttct cggcccggtt gaactcctcg
    99241 cggacgtagt cggtcacgtc cttgtagagg cgcgcctcgg cgtcggagag cttgtagggc
    99301 accgtgtagg cgatccgctc ggggaagagc ggccggccgt cgaacttgag gaggctctcc
    99361 ttgaccatcc ggcgcatcag atcggagacc tccacctggt ggacgccgtc gcggaaccgc
    99421 ccctcgaacc ggtcgccgtc gaggagcgcg agaaagagct ggaagtcctc ctctttgccg
    99481 ttgtgcggcg tcgcggtcat caggaggaag tggcgcgtga ggccggagag gagctgcccc
    99541 agcttgtagc gcttcgtgag cttgacctcg ccgccgaaga aggtggccga tagcttgtgc
    99601 gcctcgtcgc agacgacgag gtcccagccg gcgtccggcg cggcgagccg ggcctggacg
    99661 tcttcgttgc gggagagctt gtcgaggcgg gcgatcgcga ggtcgttctc gaggaaccag
    99721 ttcccggtac gggccgcctc gagcttgtcg ttcgtgagga tctcgaacgg gagatggaag
    99781 cggcgataga gctcgtcctg ccactgctcg gcgaggctcc cggggcagac gatgaggcag
    99841 cgcttcaggt cgccgcgggc gatcaactcc ttgatgaaga gccccgccat gatcgtcttc
    99901 cccgcccccg ggtcgtcggc gaggaggaag cggagcggct ggcgcgggag catctcctcg
    99961 tagacggccg tgatctggtg cggcaagggg tcgacgagcg aggtgtggac ggccaggacg
   100021 gggtcgaaga ggtgcgcgag gcggatgcgg tgcgcctcgg ccacgaggcg gtagagcgct
   100081 ccgtcgccgt cgaagctcca ggggcggccg gcctcgacga gcgtcaggcg cggctcgtcg
   100141 tgccggtaga ggacctcctc ggcgacgccg ccggtgggcc cgcggtagat gagggtgagg
   100201 gcgtccgagc cgtgccactc ggtgctgacg acggtgacga gaccgtcggg caggatgccg
   100261 cgaacggccc cctgcggctg taggtcttcg agcttcatgg gcacccctga gaccgaattc
   100321 gggctcacgc ctccggaaac gcgaccgccg cgggcattct gcgccgcggg gagctctcag
   100381 ggaacgtccc gaggcacgag cgcacgaccg gtagtgtggt gcctgcgcat gacagatgat
   100441 gtccgtgtgc tggcggagca ggtccgcctg cccaacgacc gccgtatccg tgtcagattc
   100501 gggtcaaccc accgggcagt gccctcgctc cgggacgcag acggaatcgg gcccgacggt
   100561 cgcgggctcc gtctcgccgg agggctccgg gtcttcaggg agcacgacgg gaaccggggc
   100621 gatgagagac gcggattcca gcaggcgcct cgcgagccag cgggcgagat cgagcgacga
   100681 gggagcggac gttctctcgg gccggattcc ccaatgtggc tccgcggaac gacgcggcgc
   100741 ggcagacgcg acgccgctgg cggtgagcag ggcgatgccc agggcgacgg ataccaggga
   100801 cttcttcacg acggcctcct ccaacctcac tgcggggctt gacgagggcg tcggggagcg
   100861 ctctccgatg tacctctgcg cataggctgc gcggggagag gattcttcca acagggaagg
   100921 caggttttct cagacggcgc ggcggagacg ctccgagatc tggacctccg cccgggttcg
   100981 cagcacctcg aggaggtcca cgtcggggag gtccccgcgg agggccgcct ccaacgactc
   101041 gagcacggcc gcgtcgaccg ccccgagggc ccggaccctg cgggacacct cgcccaggag
   101101 gccgcgggcg aggacggcgg agcccgacct cgcgagagcc tcgacgcgca ggagcagcat
   101161 cgtcagctcg tcgccggtcg cgccctggct cgcgaagagc cgggccgccc cgtcgaaggc
   101221 ccgcgcggcg tccccgtagc gcttcccttc gaagagggtc cgggcaaggt tcgcccgaat
   101281 gaacgcgtgg acggccggcc gggcctcccg cccgaccagc cggagggcgc gcgcgtactc
   101341 ccgtcgcgcc tcctcgacgc gtccgagccg gagcaggcag aagccgatcc cgttcagcgt
   101401 cgagacgtag gggctccatc gttcgtgctg cgcgaggacg ggcaggatcg agcggaattc
   101461 ctcgagggcc tcttcgtccc gggccaggta cgagagcgcg atggcatgcg cggccgcggc
   101521 cctcgccgct tcgtcctcca tcccgagcga ctggaacgtg agcctcgcgc gaccggcgag
   101581 gtggagggcc tcgtcggcgc gatcgaggaa ggcccggcgc tgggcttccg ccagctcgac
   101641 ggaggcgagg cggcgctcgg agacggagcc gcgcgcgaac gcgccgtagg cgcggcggag
   101701 gtcgcgtccg ccccgaagga actcgcccgt ccagttgcag gcggtgccgg agacgaggag
   101761 cgagaggccg aggaggtcgt cgagggggta ggcgcgctcg gcgggggcgt cgagcgcccg
   101821 gccgtcggca cgcgccgcga ggactcgatc ggcggagagg cgcccgaagc ggtcccaccg
   101881 cgccgggccg tcgaccatgc cgtagagcgc ttcgtcgagg gcgtaaccga gaccgtagcg
   101941 atcgcggagg cccaggaggt ccagggcttc gagggccgag cggagatcct cctcgggggc
   102001 cgcggcggcg tcgaggaggc ggctcttcac cccgatcgcc tcgcggctcg cggcgaggac
   102061 cgcgggaatc tccgtcggcc cggcggggcg cgcggcgccg cgcggggtgc cgtcgggctg
   102121 aacccgaaag gggtcgccct gcggaagagc cggctcggcg agggcttcct cgagatcggg
   102181 gagggtcgac ttcagcgtcc ggtcgagccg ctcgatgtcg cggcaggccg ccgcgcaggc
   102241 cggacacacg gcaaggtgac gcttcagacg cgccgcctcg agctcgtcca gctcgccctc
   102301 caggtagacg aggagccgga agtcgtcggg gtgtgcggcg atcatcgagc cgcccccacc
   102361 gccgcgcgaa gccgccggat gcagcgatcg agaagggtcc agacgacgtt cggagaggcg
   102421 taggcgaggg actccgccgt ctccttgagg gtcctcccct cgaggtagtg cgcccggatg
   102481 aggcgccgac acgcgggcga cgcctgggcc agggtttgcc ggaggaggac gaggacgtcg
   102541 gggtccgtgc ccgacggagc ctcgaggttc ccctccgccc ccgcgccggt ctcgagcggg
   102601 agctcgggac gcgcgcgcct cctgcgaagg aactggagcg agcggagctg gaggatccgg
   102661 aatgcgagcc cctcggcctc gcggaccggc tcttcgtggc gcatcagctc gaggagaacg
   102721 tcctgcatca ggtcttcgac ctccggagcc gagaaccgga accggtggcg gagataagac
   102781 tccatccggc ggcgaaggtc ccccggcgac gcgatggatt ccgcgagctc caaccgtgtc
   102841 cggggagtcc gaggttcggc atccatgagt caagctccct gaatgccgga attctccaac
   102901 aaccggccgc tgtcgatgga aggaatctga ccaggcgacg gacggacggc gtccagccgc
   102961 gtctccggga accggctcgg ggcggcggac ctcccgaggg ggcggctcgg cccccgctcg
   103021 cccctcagtg gttcagcggc cggtcgtact cggacgagaa cacctcccgg tagaccgcgc
   103081 gcatgtcgcg catggtggcg aggatgttcg ccaggctcgt cgaggagccc ttcccgaact
   103141 cccgcggaaa cgtctggatc ccgacctcgc cgacgcggaa gcccttgagc atcgtcttga
   103201 tcgcgatctc cgctccgatg aacgggctcg tcgagcgcag ggggagctcc gaggcgacct
   103261 ctctccggac gagccgcagg ccgcagctga tgtcgcggta gtgcgtccgg aagagccatc
   103321 gaaggacgtt gttgtagacc caggagatga agatccgcgc gctcccgtag cgcttggcgt
   103381 accggaacgt gaggaccagg tcgtaccggt cgcgcagacg ccagagcttc cggaggtcgt
   103441 cgacctcgta ctcgtcgtcg ccgtccgtga aggcgatcca gccgtagcgc gcggcggcga
   103501 ggcccgtccg gagggcccgt ccgtacccga ggttcgcccc gtggtggacg acgcgcaccc
   103561 gcgggtcctg ccgcgccagc tcttcggcga tctcgcccgc gcggtcgggc gatccgtcgt
   103621 tgacgacgat gatctcccac tcggccgcga gctcgtcgag gacgcgccgc gccttcgtcg
   103681 cgacgcgctc gaccgtcttc tcgtcccggt agaccgggaa gaagaggctg atgcggtcgg
   103741 cggagggacc ggtcaccccc gcaccctcgc ccggacgaac gtctggccgc ccaggagcca
   103801 ctggagggga cggagcttca ggtagagcga gacggcccac ggccacttcg ggagacgcga
   103861 cttcgacgtg aacgggagga agcggacgcg cagctcgacg acctcgaagc ccgtcgcccg
   103921 gagcccttcg acgaggccgc ggtccgtgac ggcgagggaa tggtcgacga agtcgaagta
   103981 gcgccggggg cagaggcgga aattcggctg gagcacgagg agctctccgc cgggcttcag
   104041 gacgcgccgg gccgcggcga gcgcccggag gagctccgcg ggcgaggcga ggtgctcgag
   104101 gaagttgctc atgaagaccg tgtcggctgc cgcgtcgggg atcccgggaa ggtcgaggtc
   104161 gtcgtccacg atcggctcga cacccggtcc ggccgcctcg gcgagggccg ggctcgcgtc
   104221 gacggcgatc cggcgcgccg cccgcacttc gttgatgaag aggcacggcc ccgcgccgac
   104281 gtcgacgacc gtcgcccgct ccgggatcct cgggccgaag aagtcccgga cgaggacgcg
   104341 ccagaccgcc cgcatctcct cgagctccgc cggcgggaag cgccgccgat agagctccgc
   104401 gagaacctct ccgggagggg accgcctctc gtcgacccgg gccgtcgtca cgcgagagct
   104461 atcgtacgac gccgttcgcc cggcgcggca gacctatact cgcgcgagtg tcgggcacgg
   104521 tgccggtcct cgcggccgca gcgacgaacg cggtcttcgc ggcggccctc ctcctcgggc
   104581 tccttttcct cggcgcggcg acggttcccc ggcaccttcg tccgtccgac ggaccgacgc
   104641 ggttcgcggc cttcctctcc ctcggagcga gcctcttcgg cgcgacgacc tgggtcgccg
   104701 gcgccctggt cggcacacgc gcgttcggtc cgctctgggt cgccctcggc ctcctcgcgc
   104761 tcctcggcgg cgcgcgctcc ggtctctccc tcctcgcgcg cctcgcccgc cggctgaccg
   104821 ccctcctccg cgcctcgccg ctcctctcgg cggccctggc cgtcgcggcg ctccctctcg
   104881 ccttccctct ctccatccct ctggtcgaca acgacggaat ccgctaccac gtcgccctcc
   104941 cgaagctcta ccttctgacg ggggaggtct tcctctatcg ctgggacgtg accggggcct
   105001 acccgcagct cgggaacatg ctcgccctcg ccggcctcgc cgccgggcgg gcggacgtgg
   105061 ccaagttcgt gaacgccggc ttcctcgcgg cgtccgcggc cctgctcgcc ctcgccctcc
   105121 atcgcggtcg cgcctcgcga gcggcctccc tcgccggccc gctcctgctc ctcgcggcgc
   105181 cggtcgtcgg cgtcgtcgcc ggctccggct tcatcgacca cgtcgcactc ttccacctgg
   105241 cggcggcgct cgtgctcgtc tcgaggaacg ggtcgccgct gctctcgggt ctcgcgctcg
   105301 gagccgcgct cgcgacgaag ctgacgcttg ccccgggcgc ggtggccgtc gggctcgccg
   105361 cggcgctcct gcggcctcgg ggcgagcgtt ggcgggcgtt cctcgtcgtc ccggcggcag
   105421 ccgctgcggt cctcgccccg ttcgcgatcc ggaacgcgct ggcgctcggc gaccccttct
   105481 accccgtcct gaccctcgcc gccggccgac cgacgccggg cgtctcgcgg acggtcttca
   105541 cgcatttcac gagcttcaac gccgggaacg ccggccctct cggcatcgtc tggacgacgc
   105601 cttcgtcggg agccgacgac gagaccgccg ggatccacaa cgtcgccggc ctcgtcctgc
   105661 tcctcttcgg cttccgggac cggctcgtcc gcctcgcctc cctcctcgtc ctcccgttcc
   105721 tcgcctacgc cgcgctgagc gcccctccga cccgctacct cctcccgctc ctctggggcc
   105781 tctccctcgt cgcggccgcg gtcctcgggc ggctccgcga gggccgcctc gcgtggctcg
   105841 cgatcccgct cgcgctgccg ggactcgtcc tctcgtggtc gtaccaggcg cggaacttcg
   105901 gcgcggccga ttacctcctc gggcgcgtct cgcgcgagga gctcctggcg cggacggttc
   105961 ccggataccg cgccacgtct ttcgtcaacg gacttccgcc ggggggtgtc atggccgcag
   106021 acctcccggg ccccctctac ttggaccggc cctggatcgt ctccggcttc atcaacgagg
   106081 cccctctcac gctctggatc cgcgagggcg acgactccga ccgcctcctc gggcggcttc
   106141 gggagcacgg catccgctgg ctcctcgcga cgcccggcta cggcggaggg acgccgctct
   106201 ctctcctccc gtacgccccc gatccttcga aagggcacgt catggccgcg ctccgggcga
   106261 agctccgcct cgtcgccacc gtcgacggcg tcgacgtctg ggaggtcccg ggcgcgactt
   106321 cagcgcgccc ttgatcgcct tcttcacctg cgccggcgag gtgcccctcc tcgagcgtgt
   106381 tgtcgacctc cacgcgcacc ggtggcctcg accggagacc gcgacccgca ggcgacaccg
   106441 ccgggcatga cctcgagggc gaatccgccg cttcgctcgg gacctggact ctctttgatg
   106501 acttccggat cgcccgcggc gcgtctttcg ggcgagcacc gcgacctgct attttgaacc
   106561 cgtctcaacc aggaggcccc ttgttcgaac cgtggttcac gaagctggag caggtccacg
   106621 aagcgaagag tcgcgaggag cgggacgccg tctacgcctt ccgctacaag atctacgtcg
   106681 aggagctcgg ccgcgagctc ggcggagtgg accacgtccg caaggtcgtc tgcgacggcg
   106741 acgacgaaca ggagtgctcc gttctgctct acacgggccg gctggacgac atcagcgcaa
   106801 ccgtgcgcct tcggatctgg cagcccggcc ggatccccag gcacgagttc gagatgctct
   106861 cgatgtcgaa gttcccgggc atcgaggaga ggacgatctc cgagctcggc cgtctcatga
   106921 tccgcccgac gctgcggggc aagctgctgc ttccgtcgct cgtgcgatcg gtctacgagc
   106981 gcggcgtcgt cgcgggggtc gacctcggct tctgctactg cgcgcccggc ctcgtgccgc
   107041 actaccgaaa gctcggcttt cgcccctacg ccgcgccgct cgtccccacc gtggacggcc
   107101 tggaagtgcc gctggtcggg atcgcgtcgg acgcgaaata cctcaagcag atgggctcgc
   107161 ccgtggcgtc gctggtctcg cgcttctacg gcacgggccc cgggaagagg ccccctctcg
   107221 acctctcgac cctgggcgac ctgctcgacc ccggcggatc ctcggtcgag ctcgagcgcg
   107281 accaggtctg gcaggaggtc caggacgggc tcctgcaaga cgccggcgcg agctcgatcc
   107341 tcgacgggtt gagcgaggcg gccgtccgga agctgaccgc gaagggcttc ctgctcgacg
   107401 tcgcgcgcga cacgctcgtc acccgggccg gccatggcca acgcgagatg tacatcgtcc
   107461 tggaaggaac gctggaggtg ctttcggccg agggccgccg actggcggtg ctggggcgtg
   107521 gcgacctgtt cggcgagctc gcgttcttcc gggacgaggg caggcggacg gcgtcggtcc
   107581 gggccgcgag cgattgtcac ctgctcgtcc tgcggcggca tttcctgacc gagctgggca
   107641 agtccgatcc ggacgtggcg ctccagctcc acttcaacct cggccggatc ctgtccgagc
   107701 ggctggtccg ggtggaacgg aagctggcgg ccgggtaggc cgggaccgcc atgcccgagt
   107761 cgggccgcac gcgggaggcg ggcgcgctcc tcgcggaatc agagaccttc gggtcgctcg
   107821 ccgagccgct ccgttcccgg ctcgcaggcg cgatgcgccg gcggaccgtc gctgccggcg
   107881 aggtcctgct gcgccagggc gaggtcagcg attcgctgtt cgtcatcgcc gccggtcgag
   107941 ctgaggtccg actgcggcgg gacggcggac ccgaggtcgc catcgacacg atcggacccg
   108001 ggggtgccgt cggcgagatg cagctcgtcg tcggaggcgt cgcctcggcg acggtcgtgg
   108061 cggccgagga aacgcaggtg ttcctgctgc ggcggcagac cttcgacgcg ctctgcgacg
   108121 agttccccga gctgctggag gcggtcgccc gggtggccgg gcggcgcgtg cggcgacacc
   108181 ggatgctgga ggtcctgccc tccctcttcg ggcccctcgg cgacggcgtg ctggctgcga
   108241 tcgagggcca ggtcgagtgg ctcacgctgc gcagcggcga ggtgctcttc cacaagggcg
   108301 atcccggcga tgcgtggtac gtggtgacca gcggccgcat ggcggtcgtg gagcccgcga
   108361 gcggcggcca ggccgaccgg ctcctcgccg aggtggggca gggcgaagcc ctcggcgagc
   108421 tgtcgttgct gaccggtgag ccgcggtcgg cgaccgccta cgcgctgcgc gacgccgagc
   108481 tcgtccgctt cccgatggac cggttcgccg agctggtctc ggcgtggccg caggtcacaa
   108541 acggcatcct gcgcaccctg gctcggcgcc tcgtgacgcg gggagacgcg tcgcgccgcc
   108601 cgacggaggc cgggctcacg ctggccctgc tgccggccac tcccggcgtg gacgtcgcgg
   108661 ccttcgcgga gcggctcgcc gtcgcgctct cccggttcgg cccgaccctc caggcgagca
   108721 gctcccgtct ggtcgagatc ggcatccaga gcgagacggc gagcgcctcc ggctcgcacc
   108781 cggcctggat ccgagtgggc gcctggctcg aggcgcaggc cgcctctcac cgcttcctgc
   108841 tcctcgtggc cgacgcgaca cccagcgcct ggagcgcccg tgccgtcggc cacgccgacc
   108901 aggtgatcct cgtcgccgat gcgaacggcg acccgagccc gggcgcgctg gagcacgcgc
   108961 tccggccgtc ggcgccggcc cgacgccagc cccggcgcct gctggccctg ctgcaccgtg
   109021 acggctcgag gcctccgcgt gacacggcgc gctggctcga cgcccgggcc gtcgatgcgc
   109081 acgtccacgt gcgcctcgac cgcgaggacg acgtccggcg cgtggctcgc gggatcgccg
   109141 gacggggagt ggcgctcgcg ctgtcggggg gcggcgcgcg ctgcttcgct cacctggggg
   109201 tggtgcgcgc gatgcgcgag cagggcatcc ccatcgacct cgtcaccgga acgagcgccg
   109261 gtgccatgtc gggttacctc gtcgcgaccg actcgagcga cgacgacctt cgacgttccg
   109321 ccctccgctt ccaccaggct cgccccttcg gcgcctacac cctcccgctc ttctccctga
   109381 tccgtggtga acggctgagc cgcgccctcg cggacgagtg cggcccgacc cggatcgaag
   109441 acctctggct ccccttcgtg gccgtctcga gcaacctgac ccgcaaggcg gtcgagctcc
   109501 acacgagggg ccctgcctgg gccgcgctgc gcgccagctg ctccctgccg ggaatcgtcg
   109561 aaccctggat ccgggacggc gagctgctcg tggacggcgg cctgatcgac aaccttccgg
   109621 tcggtgtcgc acgcgaacgg atcgtcggtc gcgtgatcgc cgtcgacgtc agctcggccc
   109681 agcgcctgac cttcgccggc agcgcctatc cctcgccctg gacgcggctg gtgacccgct
   109741 tcggccgccg acagggccac ccttcgccgc ccggtgtgtt cgacgtgctg ctgcacagca
   109801 tgctcctggc cagcctcgcg aacgtcgagc ggatgcggct cgatgccgac gtttgcctgc
   109861 ggcccgagct cgcagggttc ggcatgctct cgaccggcct gcatgcccag atcatcgacg
   109921 ccgggtaccg ccacgccagg gagcacctga agtccttcga cccctcgctc tgatcccgcg
   109981 aacgccgggc gtcggaggcc gccgcgacgc tccgcgtcgg cggcggggag ggccttgagc
   110041 cggttcccct cggaccggcg tcccgcggcg gcgtgaggcc gcgcggcgga gcctccgctc
   110101 cgggccgagg gggtcagtac gggtcgtaga cccgggtcac ccgcgggttg acctggcagg
   110161 tcacctcgta cgtgatcgtg ccgagcttct cggcccattc ctcgcaccgg atctcggcgt
   110221 cgccgctgcg ccccatcagg acgacctcgt cgccgacgcg cacgtcggtc tccggcccga
   110281 ggtcgaccat gctctggtcc atgcagacgc gcccgacgag cgggtagctc cgcccgccga
   110341 tcaggacgcg gccgcggttc gagaagaggc ggttgaagcc gtcggcgtag ccggcgggaa
   110401 tcgtggcgat gaaggtgtcg cgcggggcca cccaggtgcg cccgtagccg atcgaggtcc
   110461 cggccgggac ccgcttcagg aacgagacgc gtgtgacgag gctcattccg ggcttcagag
   110521 ggatcgtgtc gggcgtggcg gggtcggggc ggaagccgta gatcatgatc ccggggcgga
   110581 ccatgtcgag ccacgcggct tcgtggccga ggacggcgcc ggagttcgcg cagtggacga
   110641 ggagccgccg gccggccgcc tcctggaccg ccgcggcggc cgcgcggaag cgggcgatct
   110701 gctcccgagt ccaggccggg tcggccgcgt cgctgacggg gagatgcgtg aagacgccgc
   110761 cgagctccac gcccgggagc gccgcgaggt ggagcgccag cgcgggcgcg tcttcctgcg
   110821 tcacgccgac ccgccccatg ccggtgtcga ccttgaggtg gacggcggcg gggcggcctg
   110881 cggcgcgggc ggcctcggcc gccgcgtccg cctcctctcg cgagcagacc gcgagcgtga
   110941 tgccggccgc gacggaagcc tccatctccg cggggaacgt cggcccgagc ttgagaatcg
   111001 ggaggcggag gcccgcctcc cggagctgga ggccctcggg aacggtcgcg acgccgagcc
   111061 agtccgccgt cccggtcgcc tcggccatcc gcgagacctc gacggcgccg tggccgtagc
   111121 cgttcgcctt gacggcgacg aggaccttgc gctcggcccc gaccgcggcg cggatcgcgc
   111181 ggaggttgtc gcggacgttc ccgaggtgga ttcgcgcgtg cgtggcgtag aggaggctca
   111241 tccgcggatc acgacgttct ggacgtaggg gctcgtcgag agcgcgagga gcgcgccgta
   111301 gccgggccag aactcgagct cgtcgcccac ccgcacattc tccgccgcat cctcgacgtc
   111361 gaggatcaga tggtcgctgg agccgccgag gacggtgacc ccgggcagga ggggctcgat
   111421 gccgtcgacg acgacgtcct gtcggccgag gttgcagatg gcccggagcc gcacgccccg
   111481 gtcctcgaac tccggcgtcc cgccgaaggc gtcctggccg cgctccccga tcggcatcga
   111541 cggcttccgc tccacctcca cgacctccgc gaccatcttg aacgtgtcct gccgcgtccc
   111601 cggccagggg ctccggtcga tgacgttgcg gccgaggacg atcgcctcgc cgattcggaa
   111661 gtggttgatc tcccacggca tctgcccgct cgcgaggagc gggaggttcg cgctgttgcc
   111721 gccggagagg acgttcaggt cgagccccgt ctcctcccgg caccggttcc tcacctcgac
   111781 gagcgcggcc atgttcgcct tcgtcgggat gacgccgccg tagcaggcga ggttgcagcc
   111841 gaggccgatc acctcgacgt tccggagctt cgaggcgccg cggacgacct cgatcgcccg
   111901 gtcgggccag accccttcgc gcaggtcgcc gacgtcgacc atgacgatca cctggtgcgt
   111961 cacgccgtgc aggaccgccg cctccgagag gagctcgatc gtcccgaggt tgctgttgag
   112021 cgtgacgttc gcgcagcgga cgacgtcggc ggcccggctc ggcgccggga tccggaggag
   112081 catgaggggc cggtcgatcc cggtgtcggc catcgcctgg aggttccgga tgcggctgtc
   112141 ggcgaccatg tcggctccgc ccgccaccag ggcgttgacg accgccgggt gggcgcagac
   112201 gaccttcgtc accgccgcca cctgcgcccc gtgctcgtgg cagagggcga tgacggcacg
   112261 cgcgttctgg gcgatccggt cggggtagat ctcgaggcgg ggggtccgga tgaacatggg
   112321 gctcctcagg gccttgccgg cgcgtgcgct tcgacccacc gtcggaaggt cgcgcggacg
   112381 tcgtcggggt aggcgtgggc gagagtgccg agcgcgctga agcggtactc ggcgtcgacg
   112441 aggatcgtgg cggacgggtc ggggaggagg tgtcggcccg cgccggtgca gacggcgcga
   112501 aggcactccg ccgccccctc caccttcgtg agggcgccgc cggtcgcgac gacccaccgg
   112561 acggcggtga ggtccttccc cttcacgacc tgcttccggc cggtcggggt gtagaggtcg
   112621 gagacgaccc ccgcatggcg gcgcgcgccg atcgagacgg cccgatcgca gagccagcgc
   112681 gtgagcgcct tctggcgttc ggtgacgggg agagcggaga ggtcggcgag gcgctcctcg
   112741 ccgtccggct cgtccgcgag cgcgacgacg tttcgggcgt tcacgtagac gccgaggtcc
   112801 ccctctacgg tccgcttcgc gcgcggctcc ggctcgacga gacgggcgtg ccagtcggcg
   112861 ctcccctccg tcacggagtg gacgtcggtc gtcgcgccgc cgacgtcgaa gacgaggcag
   112921 tcgcccgcga cctcggcgaa gagctcggcg ccgcggagga cggcgcccgg cgtcgggagg
   112981 acctcgtggc gcgtcagccc cgcgaggcgc tgcatccccg gcgcgttcac gatgtgacgg
   113041 ttgaacgcct cgtgaacgag ccgacggagc gggtcgacgt tcaggacgtc gacgtcgggg
   113101 aagacgttct gggcgacgtg gagctcgacg cccgcgaggc ggaagatctc ggtgaccggg
   113161 cgccggagcg cggcgttccc ggcgtagagg accgggacgc ggaggccgcc cgcgaccagc
   113221 gcggcgagct tctcggcgtt cttcaggacg atccccttct cgccgtggtc gacccctccg
   113281 gcgaggagga cgaggttcgg ggagatcgcg cggatctcgt cgagctccca gctctcgagc
   113341 tcgcccgccg tcaccagctt cacgatcccg ccggcgccga gcgccgcctc gcgggccgcc
   113401 cgcgccgtca tcgacatcgt caggccgtgg acggtcatcc ggaggccgcc ggccgccgag
   113461 ctgctcacga aggtctcggc gccggagggg tcgaggccgg accgggcccg aaggtccgcc
   113521 agcgccgcgt cgacgccgat cccgacgtcc cccgcgccga cgctcgtcgg ggcgaagccc
   113581 tgcgcgacgt ggtcgaagcc tccggccgcg aggcggacga agccgttcgc cttcgtgatc
   113641 gtcgagccga cctcgagcgt caggacctcg atctgcatct gaagcctcgg gccgcgcctc
   113701 ccgatgggtg gatcgcgggg cgcggccggt tcccggccgc gccccgccgc tgcgttccgc
   113761 tcaggccgct ccgcgagccc ggagcgtctt caccatcgcg ttgacgacgt ggattccctt
   113821 cgtgccgcgc ccgaacgtcg cgtcgaggcc ggtctccttc gccatctcgc ggttcacctg
   113881 cgtcccgccg gcgacgagga cgacgcgatc gcggatcccc ttctcgcggc agagctcggc
   113941 gagcttcttc atctgggcac ggtggacgtc gttgtggctg atgatcgtgg agatcaggat
   114001 ggcgtgagcc ccggtctcga cggcggcgtc gacgagcttg ccgacgggga ccgacgtccc
   114061 gaggtagtgg tacttcacgc cgtacttctc gatgccgccg tgcttgatgt cgaggatctc
   114121 gcggaggccg acgctgtgct cgtcctcgcc gacggtaccg gcgacgacct tcagaccgat
   114181 cttcttcacc gcgtcgcgca gctcctgctc ggggaggatc gtctccttct ccgggatctt
   114241 cagcgtcgtc cggtcgacgt cgaacgcgac cttccccttc acctcgacga agcacccctc
   114301 cgccgggtgg aggaccatcg tgttgatgac ctccgggtcg acgagcccca tcttcctcgc
   114361 catctcgacg gcgtagaccg gggcgatgtc ctcgttctcg gggacgaaga gctggacgag
   114421 gaccgtcccg tcgccggccc actcggcctc gggcttgatg gccgtcccct tgcggtacgg
   114481 cagggtcttc tcgaggcgac ggtggcagtt gtcctcggcg tcgagctcgt cgatgaagac
   114541 gatcttcgac gggtcgcaga gcgtgcagcc gccgatcggg tcgcaggcct tcgccgcgcc
   114601 cgcggggagg tggttcgtcc cgaagtggtc gcagacgggg gccaggtagt ccgcgtcgcg
   114661 cgggacgagg gagccggc
//
LOCUS       DAOONL010000002        86350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_122_length_86350_cov_17.733102, whole genome shotgun sequence.
ACCESSION   DAOONL010000002 DAOONL010000000
VERSION     DAOONL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 86350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_86350_cov_17.733102"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLP50_00465"
     CDS             complement(<1..607)
                     /locus_tag="PLP50_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPA50044.1"
                     /translation="MTHADRPLAAGRRTSGGPLALLLLLPLLGTLASCSTGRPGPGSS
                     GAARAGSLPRRAPAVAALTLREKAAQVVMVASRGVYRAEDDPEYLELARLVSEEGVGG
                     VIWFLGTPLETADLNHRLNAKAKLPLLVAADLEAGPGMRFPDLVLGPSAMAVAATGDL
                     ALAARRARATAQAARALGIRVVFAPVADVNNDPDNPVINVRS"
     gene            complement(647..4969)
                     /locus_tag="PLP50_00470"
     CDS             complement(647..4969)
                     /locus_tag="PLP50_00470"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA50045.1"
                     /translation="MQHRRRFLLVASALLFAADSAMAAGPTTPPVLDGPIPFSGKRNL
                     PNLVLLGAPTERAHEKEATHEGRRYWEARREWERLERAEIPAAPILRSVAQEMYRERL
                     RFRTPDGGPQPLNATLAAAGWKSLGPTTDAGRVRDYAFTRDGSKLYVATANGGIWLLT
                     RQGGPGADYANPVNLTDDLPLLTFGAVAVAPSNPNVVYAATGEQSPLSGSQVIGLGTL
                     RSNDGGSTWSFNTISVAGGAFDVIPSVYSYDLDVHPSNPEDVVLGTANGIFRSTDGGR
                     TWSNRLPATGEGLEWRRQGVNIARSPANPNVLWAGLWGGLAVSTDAGSTWQVAFEDIA
                     QQVGFNGIPIRSLVAIAPSDPNRIYWLVAGYDQQRGYSQVGVLRSDNGGQNWGVALGP
                     PAGQGYPLISGTQGWTFLGLAVDPTNRDRLIAGGLDTWRSEDGGLSWTQISQWTLPER
                     HPQFCHADVNVIAFEPGLSSFWMGTDGGLFRSTNGGRSFIWKNDGVVARMFSSLAQHP
                     TDPYRLYAGTQDNGTMRLSGDSTSAWKRIFYGDGYDCAVNPQNPNIVYATNFNGYTSR
                     ADDGGESEESFRLTTCPADAQTEDQCSVPPVTSFRSRLAMDPVDPRVLYTMTDRIYRT
                     SNGAATPSDWQPVFAEYFCSDGVSAQPCPNVQKGWASCSSISIRPTDRNRVAFGTAAG
                     YVVYTLNGFQNGSLVNIGSQVNAIAWDPADGNALYVGLESATEVVQGQGRHVIWRIAG
                     LNQQQQTASPASSGIGVPITYAGGSFTFFAPVDSLVFSPTDPNLMFAGTKYGIFRSTN
                     KGQSWSRFGDDFPATWVSYLLFSPDGSRLRAATWGRGMWEINPAGGTPSPTSPPTPDF
                     SFAPAQPKPGQSVSFSDRTQGGAATWSWSFGDGTTSTAQNPTKVWGNPGTYSVTLTAA
                     NGAGSRSVTRGVTVSYGSTGTGDAYTYLLPIVLTSSGAGGAFFTSELTLTNRSGRTLN
                     LTFRVKGSFEASSTYSLPPGQQIHPDIFAFLQASTGMSVPAGNKTASLRIEVRGADNL
                     VQFGAQVRVTTPATADLRAKGVIGRFGLAFPATPLGRAASGEAFVFGLQQTSAAGSAG
                     TRSNLACVNAGGGGLSAVRLEVTYYNGDTGAAHPTKDFLDLAPFQWEQKSTPLASRGL
                     TYGYAVVRRTSGSDQFVCYGVLNDNLNGDGAFVPMVPNDTPSRTSAALVPVVLEAAGY
                     SSEMTFANRTSRDISGLFALVPRTTPVPDWGYFDLPARSQFTVPNVVAELRRIGFSAP
                     AGTVASVFAQFLDGQFREDQSDTQAPIPTSDGFLGVRTTTTRAGGLLGLAYGYTPIGD
                     AADTEAWVYGLQQAGTRGQEGGTRANLAVGHALGGQVEPLQLEVTYFGPSGSELGKEP
                     QCSPCTLAPGQFLQFDSALARFGVTQGYARIRRVAGSDQFLAYGVLNDQGNDDGSFVP
                     MIVP"
     gene            complement(5148..6452)
                     /gene="lysA"
                     /locus_tag="PLP50_00475"
     CDS             complement(5148..6452)
                     /gene="lysA"
                     /locus_tag="PLP50_00475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPA50046.1"
                     /translation="MTLRDGLLRIRAGEAAHPEAVSALTRLAAGHGTPLYVYDAETIR
                     ERARALHAAFAPRFPRLALRYALKANTNVEIVRQLLAEGLAPEVVSEGEIRAALRAGA
                     TGHDILFTSSSKSPSEIDFALGHDVLLNVDNLDELEQVSAAALRLGTTARISFRINPG
                     VDPDTLHQINTGIPESKFGVHLDGGHARAAYAKARALPALAIAGVHCHIGSQITETAG
                     YEKTARRMLAFVRELKDELGLTLSFVDLGGGLGIPFADGQEVMTPEDLARALKPIWDD
                     EVSRLGYEPELWIEPGRYLVAQAGLMLARVNSVKTTPVKTFVNVDAGFNTLMRPALYG
                     AAHRVRIVGRDGEPMLLDVAGDVCETGDILAEARLLPRPAAGDLVAFLDAGAYGFVMA
                     SEYNARPLPAEVLVDGDAVRVIRRRGTWEELHRSEPEGVPEA"
     gene            6600..7658
                     /locus_tag="PLP50_00480"
     CDS             6600..7658
                     /locus_tag="PLP50_00480"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPA50047.1"
                     /translation="MRTRRSLLSEAALASAFLALPRSARAAAPPSGPVRPKSLAPGAR
                     VALVSPASPGAEAESAVIAEEVVASLGLLPKRMPAAARQTMYLAGTDAERAADLNAAF
                     RDPSIDAVWCIRGGYGSGRLLPLLDWETIRRNPKPLLGFSDITALLNGFYAKTGLVTF
                     HAPTCSENLSDYTLAELKRVVFTPEAAGVVAAAPPAEPKEGFVDREDRLRRIAPGSGR
                     GRLVGGNISVFSMLVGTPWEPPLAGSILFLEEVGEEPYRIDRWLTHLVLTGRLAKCAG
                     IVFGKFKDCGPGDEGSYQGTWTWQEVVADRLGKLGIPVLAGLRFGHVADKSTLPVGVL
                     AELDVAAGTLSLLEPAVS"
     gene            7655..9373
                     /locus_tag="PLP50_00485"
     CDS             7655..9373
                     /locus_tag="PLP50_00485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPA50048.1"
                     /translation="MREGRRARRAGRAARALLLAVSLAPSVASAGPDRTPAGLPRVLD
                     GGWRAADADPPDGIAGIDRLDFRPADPVSDQAPRGEVRWYRILVDLSPFVGQPLAFAA
                     DGLRDVDEAWFDGVPIGGFGAFPPGPETAHFVSRVYPLPTDRVDEAGPRELVLRVWHG
                     KRDGSVFRSPPVIDRLDRLERSRSLRDQSLVLFVSAAFVVAGLLFLFAFHARSPADYL
                     LFAGFAVALGLWVTTGHSRWGELPFPLSTVFRAGLVALSATAVCYGAAMLRFLGGMRP
                     VHRVLLPALGLLAPLAVFLPDAEDLVLPVFVFRWVFAALLVDLLVRFGIAAFGRRRLA
                     RTDLLGHVAFVLAAVPVAGLLPGAELPRIDLSGRVLMFGLLFLGLASTVLLRMSEEIR
                     RYRVAGLTDPGTRLWNREALYDEIAERGEAVRRGKGAGFGLLLADLDRFREWNDTKGR
                     VAGDRVLLRAARALIDASRPNDLVARYGGDEFAVVVGDVDRVSLPALSERLRSAIGEA
                     VKDESGGLLTSASIGTELYDPERHRTPEDLLREADRALWEARERSRGTTPTVRQTPSG
                     KWSLRL"
     gene            9397..10824
                     /locus_tag="PLP50_00490"
     CDS             9397..10824
                     /locus_tag="PLP50_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA50049.1"
                     /translation="MTKHVRRLALPLSALLLSIPIRADGPLPVDTRAAVGALAARGAS
                     PTRAVEWVRRLTDEVGARPSGSPGDRAAIAWGQALLRELGFVRVRAEEVPVSVWERGV
                     ETAEVVAPFRQPLVVTALGGSVGTPAEGIAASVVRMESLEALEEAVKADPDVVRGRIV
                     FFARRTMKAGQGPGYGANVPMRTTGAAKAGPHGAVAVLVRSVGTSRSRFAHTGSTRYE
                     AGGPRIPAAAVSNADADLLERLLARGPVTVRLVLGCRPLPDGTSANVLGEVRGRERPD
                     EVVVLGAHLDSWDLGTGAVDDGAGCAIAIEAARLIAEAPRRPRRTIRVVLFANEESGG
                     AGGRAYAERHAAELPKHAAAFEADSGTGTPLGFSWNAAGTLEPALAAVAELLRPLGAE
                     GLRKGGYGGVDVAPMAPAGVPLFGLRQDSSTYFDVHHTADDTFDKIEPASLDRSAAAM
                     AAMAYALADLPRIHARPAEPATEGR"
     gene            10821..13112
                     /locus_tag="PLP50_00495"
     CDS             10821..13112
                     /locus_tag="PLP50_00495"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPA50050.1"
                     /translation="MTLPSLPSLPAAGGAPAAAPPPAGPEVELAIAGMHCASCVGRIE
                     TALGRVPGVSLAVVDLLSGRARVRLSDPSLPVERLLEAVVSAGYEAKPAASGESTGLE
                     ELVDAEQAERASQLAALTRKAAFAGVVAVPVALLSMAEVTFPGRDVLFLVLTALVLAV
                     PARDILVSGARAAARLAPDMNTLVALGTTAAFLTSAAATLFPGALHAEGEHAPVYYEA
                     AVVVVALVLLGRFLEERAKGRAGEAIRALARLGARKARLVRDGGDVEVPLERVRAGDL
                     LRVLPGEKVPTDGVLVEGSTAVDESLVTGESIPLEKEPGDPLLGATVNGNGAVVMKAT
                     RVGKETALAQVVKLVRQAQATKAPVQRLVDRVAAVFVPAVVAVALLSFAGWMLFGPEP
                     RLLRALLAVTSVLLVACPCALGLATPTALVVGTGRGARMGLLLRDAGVLEKAARVTAV
                     VFDKTGTLTAGRPAVRAVRPVDGTGEADLLGALAALESGSEHPLGRAIVAEANARGLA
                     FERATDVKAVPGGGVTGTVGGAPALAGSEAFLASRGVSVPEALLGEADGGTLVLLAVG
                     GALRGAVTLADPLKAHAREALAGLSARGLSLHLLSGDRPAAVAAAAREAGIPEANVAA
                     RLSPSEKAERVRALRASGAVVAMVGDGINDAPALAAADLGVAIGSGTDVARAASDVTL
                     VGGALAGIGTAIDLSNATLRNVRQNLFLAFAYNVVLIPLAAGAFYPFTGWTLNPMLAG
                     FAMAASSVSVVTNALRLRRFGKG"
     gene            complement(13121..13918)
                     /locus_tag="PLP50_00500"
     CDS             complement(13121..13918)
                     /locus_tag="PLP50_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPA50051.1"
                     /translation="MALPLHLVDAFTDRPFAGNPAGVCLLREPLPDAVLQSVAMEMNQ
                     AETAFLLPEEGEWRLRWFTPEVEENLCGHATLASAHVLFEAGLLAPDATARFRTKSGL
                     LTAVREGATIVLDFPATPAEPFPPLEKAIRALGISPDFSGKTRFDLFFEVAEEAEVRT
                     LRPDLALLEEVPYRGVIVTARAEAGSPHDFVSRFFAPGCGVPEDPVTGSAHCALGPYW
                     GEKLGKDDLVGYQASARGGTVRVGRRGERVTLAGSAVTVVRGELALP"
     gene            14029..14604
                     /locus_tag="PLP50_00505"
     CDS             14029..14604
                     /locus_tag="PLP50_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA50052.1"
                     /translation="MSTVPDDDADLMGRVKAGDPAGAEGLFSRYAGPILGFTERMLGS
                     RAEAEEVTQDVFLKMITRCEQYDGRASVSTWLFAIAANACRDRLRSAWRTTSVPLEAV
                     PEPKGEGSIEGGLLQKEQRRVVRAALDRLTREQREALLLARYQGMPYAEVARTLGISE
                     GAVKTRVFRAVETLRALFAEEGGPSWTAARS"
     gene            14583..15560
                     /locus_tag="PLP50_00510"
     CDS             14583..15560
                     /locus_tag="PLP50_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPA50053.1"
                     /translation="MDCREVLETALARTAGEPADTRQAELEHHLERCESCRRAAAALD
                     ETWERIGLPEPDVSIAFRERTIGRMQEALARRNVVSFRPRTWWRPVLQAAALLVTGVG
                     GFLLARASAPPADFPAAAVERAGTPSVAVVSERQLDASRVALDLSGNPRLLNVAFRPS
                     AGDGRLSISFDVTTRYHVSGRAEDRAVAALLAYVVDAGAATEGARGRAIDLVSRELGE
                     RAAASPEIVEVLVRTLKDDGNPGVRKKAAEALVQLPPSPAIRDALALALRRDTNPAVR
                     MIAVEGLAKAATTLRDPLSIETLREKAGDETEPGFVRVKAASALREIPL"
     gene            15732..17666
                     /locus_tag="PLP50_00515"
     CDS             15732..17666
                     /locus_tag="PLP50_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPA50054.1"
                     /translation="MNTETTMTTMSTRSVSRTWTVFWILLIGAFLVVSLAWPARADRL
                     GATASRTETFTAAAGVKSLTIDGVSGDVEVAAGASFAATVEVTVKADGDARAREILGK
                     TRIAFENSGGELTLATVEPGAEYSREGRRTRLRVSHDGDRRWRVEARYRVTLPAGASA
                     TVSLVNGGIVTKGLDGVQELTTVNGRVAASDARRSATLKSVNGTVEGAFTVLPRDTKV
                     VAETVNGGVKLTLPSDAAFDLKASAINGGIRSTFTLPVRGGADDDVAVREIEAAAHAE
                     AERARAAADRARERSRKARAAGSSADESADSWDREWKEFGREMAEFGREMARLSREIS
                     RSVSGSLNRSYEGTHRGGGAAVKCSTINGGVLVLSSGSAESAAKSLVPKRGEGWAGAR
                     VAPVPPAPPVPPVPPVHVGRVHGREDGSVVKGDVSGDLHLSLPTGDVRAGRVSGRATV
                     RTSSGDIRIGAVGKGADLSSSGGDVRVERVDGGLTASTLGGDIVVGDASGEVRLRTMG
                     GDVRLRSASGPVTAKTAGGDVRVLRALGAVRAETAGGEVYCEIASKDAGPVELSTGGG
                     DVTVVLPANFRGDVEVVVRSVDADGEYVLSDFSEIGILRQRGQGRGVVRAEGRLNGGG
                     AKVTVSASSGTVHIRKGPPA"
     gene            17803..18327
                     /locus_tag="PLP50_00520"
     CDS             17803..18327
                     /locus_tag="PLP50_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA50055.1"
                     /translation="MKRFFPAALAALAMLVVAPASVAAIPEKGSISIFAGVGPAIPFE
                     GDYKVGFHLEAGGEYYLSNVLALRGTLGLTRSNADGGSGVTLGGLEASAVYYARRGKW
                     SPYVLAGVGIHTVDPPGRSASQRLSAHVGGGTEYYLDRRTALTGEITGRFIGSAGDRT
                     SSFASLAVGIKYHF"
     gene            18493..18840
                     /locus_tag="PLP50_00525"
     CDS             18493..18840
                     /locus_tag="PLP50_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50056.1"
                     /translation="MALVAALLTLLGVQLYLPTAVAACGLAETDCCCAAEAAGSAEKA
                     PADDCDCSISRPAPAPAADVAPAAAFAPPALSAETVDERRTEGPATGCRLPTPPSRAR
                     SAPTQALLETFRI"
     gene            18912..20300
                     /locus_tag="PLP50_00530"
     CDS             18912..20300
                     /locus_tag="PLP50_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA50057.1"
                     /translation="MKLVPWFPRRFAPATLAVAVLSLSPDVPARAAVAAPSPAPVPAP
                     VRETASTVEALVAEALERSPALAAVRASVAAARERIAPAGTLPDPMLRLSYENDGASL
                     SLGTEAMTRLSVMAEQPILWPGKLSLAARIAEADADVAEAAIARLGLGVEAQTRRAAA
                     RLLEARAAGELTRELSENWDRIESVARSRYAAGLGAQQDVFRAQAERTRLLQQLHRDE
                     GAVHAAEAELRRLLLREPGTPVELEERLHETRPEPPDADVELARAEAESPEIEVARRA
                     AEREALAIDLARRDRMPDLVASVGYMNRGSLPMMWAVGLGVTVPLFSGRKQEPRLREA
                     QARLAAVRAAEADVRLRLRARTEAQLALLAQLAEEARLDREALLVQDRMAVEAALASY
                     ASGSVPFVTVLEAISTEFADRRAALGRLAAFLVAEADLYERSLDGAPMAAASALPSAP
                     APPAAMNAAGGM"
     gene            20312..21766
                     /locus_tag="PLP50_00535"
     CDS             20312..21766
                     /locus_tag="PLP50_00535"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA50058.1"
                     /translation="MKHSRIVLVALVALAVPTVFLAAGCGGTKSQIAVAKTWICPMHP
                     DVVKDGKGSCPICGMDLVPKDDATPVPQAASHETWICPMHPEIVKDRKESCPICGMDL
                     VKREEDASAGGAGEAGVPGFATVTIDARKRQLLSLKTARVDRGTFGTGIRTVGRVAFD
                     ERRVHHVHTRYEAYVEHVAADFTGKYVRRGEVLAHVYSPELFATQQELLLALRAVRAL
                     GPSADATAREGADRLLDAARQRLRLWEVTEKDLAALETRGEPLRSVPIYAPTSGYVTA
                     RTAFHGMKVMPADTLFDIVDLSSVWVLADVYESELPRVSVGQTGRMTLAYRPGRSWTG
                     RVTYIYPSLDEKTRTAKVRLEFANPREELKPEMFADVVLESAPRTALKVPDDAVLDSG
                     TRKVVFVAKGEGVLEPREVKVGEKGGGAWEVLSGLEEGEEIALGASFLVDSESRLAAA
                     LAGMRDGATPAAGHEGHAPAPSPSPSSPGTGHDH"
     gene            21777..25109
                     /locus_tag="PLP50_00540"
     CDS             21777..25109
                     /locus_tag="PLP50_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA50059.1"
                     /translation="MIRKIIRFSAENKYLVLALWAVAVLLAFRSLRNVPLDAIPDLSD
                     TQVIVLSKWDRSPDILEDQVTYPIVTALLGAPKVKTIRGFSDFGYSFVYVIFEDGTDL
                     YWARSRVLEYLSKIQGSLPQGVKTEIGPDATGVGWVYQYALVDETGQTSLDELRSYQD
                     WFLRYQLQSVPGVAEVATVGGLARQYQVTVDPNRLAAYGLSLETVSDALRRSNDEVGG
                     RLIEISGREYMVRGRGYLKGTADIEKVVVKTDERGTPVTIKDLGSVALGPDLRRGLAE
                     LDGKGETVGGIVVMRSGENALHVIERVKERIDELRPSLPPGVKLVTTYDRSTLIESAI
                     DNLKGKLVEEIVIVSIVILIFLWHVPSALVPILTIPVSVLLAFIPLHLMGITVNIMSL
                     GGIAISIGVLVDGAIVEVENAYKKLQIWNDSGRKGDFHAVRLEALMEVGPSVFFSLLV
                     IAVSFLPIFTLVDQEGRLFTPLAWSKNFAMALAAVLAITLDPAMRMLFARMDPFRFGF
                     AHDGHGALARLVRLPARLVEKAVNAVAVGTYHAEERHPVSKVLFRLYDPVCRAVLRHP
                     KKVVAASLLVLVSTVPVYLSLGSEFMPPLHEGTILYMPTTLPGISVTEAAKLLQTQDR
                     VLSTFPEVERVFGKAGRAETSTDPAPLSMMETTVILKPENEWRPKERWFSPWAPEWLS
                     AVLRHVWRDRITYEDLIGEMDAALKIPGSTNAWTMPIKGRIDMLTTGIRTPIGVKVFG
                     SDLAEIERIGAEVEAAVRTLPATRSAFAERVAGGYFVDFDLKRDALARYGLTAADANM
                     AVMTAIGGETVTTTVEGRARYSVNVRYPRELREDLSSLSRVLVPTMSGAQVPLGQLAD
                     IRLVTGPSMIRNENGLLSGYVYVDFDTSKEDVGRFVEKAKAAVAGRVSVPTGYSLVWS
                     GQWENMVRVRERLQVVIPVTLVLIFLLMYANTKSVTKTLIIVLAVPFSVVGAVWYLWL
                     LDYNMSIAVWVGMIALMGLDAETGIFMLLFLDLAHDEAKKKGLLRDLPDLVEAIVHGA
                     VKRIRPKAMTVLSAFFGLLPIMWSTGVGADMMKRIAAPMVGGLVTSFALELLVYPAVY
                     LLWKKRELPGPGAPGGEAAAAA"
     gene            25184..26242
                     /locus_tag="PLP50_00545"
     CDS             25184..26242
                     /locus_tag="PLP50_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPA50060.1"
                     /translation="MTASPAASAGGHGDEGPASLERRLRSWRPFPHEVAAAVYIAAGI
                     FILERLPVTYPREALLLAASFPIAWIAGFTAVLLAGREVARRLRGRSRPVAFATEALI
                     LVRAALVLAPVLSVHFLLKSFIWLVNPRTWDAQLWELDRIVHFGLSPTLFLTVAFADG
                     GFLRFLDVVYSGAYFFLIVVSVPVLLVVPQPSRRMAFLAAYTFIWMAGSVLYLALPSW
                     GPVFVVSDLFSGVLRHMPATVSVQEVLFGEISSLVKQPLAPRIVRFGSVAAFPSLHLA
                     VVTVFTVASRWVSGRWFLANLVLVVLMLVGSVVTGYHYLVDSWAGIALGLAACSAGSR
                     LFPSPCAATCGPDSPQRP"
     gene            complement(26230..27762)
                     /locus_tag="PLP50_00550"
     CDS             complement(26230..27762)
                     /locus_tag="PLP50_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HPA50061.1"
                     /translation="MIPKPPFVSELTLERELEEFDRLKPRLREVWDSFTMRDEEPHTS
                     IVVPSLTLDQEELLKLEGSPYYEERLLFLLIRLRNPRARVVFVTSQPVHPLVLEYYFQ
                     LLVGIPASHARARLTLLCAYDDSPRSLTEKILERPRLIERIRAGIADPARSYLTVFNS
                     TPLERKLAVLLGVPLNGVDPSLRHLGTKSGSRKVFREAGVPLPEGFEDLRSLEDVEEA
                     LLELHRRRPRTRKAVVKLDDSFSGEGNALFRYPTTVGRETVREAMQRFEYAVPWETNE
                     DYFSRFARMGGIVEEFLEADQKTSPSVQLRVDPHGAVVPISTHDQVLGGVGGQVFLGC
                     LFPAADGYRLEAQAAAHRIGEVLADRGVVSRFGVDFLATRTDDASHWSLHALEINLRM
                     GGTTHPYLALQFLSGGRLDPGSGLFFSPRGLPKFYRATDNLKSEAYRGLLPEDLIEIL
                     TINGLHYSHNTESGVLFHLIGALSQYGKIGVTAIGNSREEVEHLYARTLSILDRETSY
                     GR"
     gene            27840..28331
                     /locus_tag="PLP50_00555"
     CDS             27840..28331
                     /locus_tag="PLP50_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPA50062.1"
                     /translation="MSNRRRAGFTLIEVLVVVAIVGIVVAIGVVNYLNAIERARQRRS
                     MADMRGISTAIEAYASDLNRYPPASAFVLPAGLDLPTENLVAVRGYLQPTYIRALPLV
                     DGWNSWFTYGTSSSFSDYVLRSSGRLGVAQTDPPFAVTTDFRDDIILVNGRFVQWPEG
                     VQH"
     gene            28503..28994
                     /locus_tag="PLP50_00560"
     CDS             28503..28994
                     /locus_tag="PLP50_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPA50063.1"
                     /translation="MKRNTKGFTLIELLIVVAIIGIIVAIAIPNLLNAIQRAKQRRSM
                     GDMRTTATAIEAYAVDLNRYPPAAAYTLPDTVIPDATLAGISEYLSPTYIKQVPLADG
                     WNSWFLANVDSSGSLYSLGSAGRDGKYTAKTATLLGPTTDFNADIVYVNGSFIQWPEG
                     VQR"
     gene            complement(29091..29504)
                     /locus_tag="PLP50_00565"
     CDS             complement(29091..29504)
                     /locus_tag="PLP50_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50064.1"
                     /translation="MPHELKTSPDEMELLVTSPDREGLFRDALAGALEAVYGAPLPEG
                     ENQGQVVPVQASAGDDESLLVELVHDVLRAVRAVDGALRPPRWLAFDDRRVTANLPVH
                     LPKVDVRLLQVSGAAVETGDGGWIARIELLPRVEA"
     gene            29575..30681
                     /locus_tag="PLP50_00570"
     CDS             29575..30681
                     /locus_tag="PLP50_00570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPA50065.1"
                     /translation="MSDARAPSLTDRLRDWIVREGPLRFDRFQNAALYDHEAGYYERP
                     GRVGRGGDFVTGASWHPAFARCLVRAATGVGQELGEAVDLVDVGSGEGELLAFLEEAS
                     AGNGWRLRGVEASAARRTAAHRRAPGATFHSTVDELPWGLRGLVVAYELIDALPVRAF
                     RLGADGALRERCVGLDGEGRFAWAEQPCPDGPSILAALARRGAAPEPDQLLEVRPGTA
                     ALVRAIASRLDRGLLLFFDYGAPTRALYGPARVNGTLEAFLAHGVSRDVLTDPGSRDI
                     TAWVDFGEVEEVLRTEGLVVHGLVSQSRFLLATGIAEELALVPGEGVDAHRVAERNAV
                     AKLVSPGGMGESIRVLVAERETGLGKSLISGPLV"
     gene            30716..31141
                     /gene="ndhC"
                     /locus_tag="PLP50_00575"
     CDS             30716..31141
                     /gene="ndhC"
                     /locus_tag="PLP50_00575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPA50066.1"
                     /translation="MTTRTTQGRKRPRGGACAGGAEAVQSYIPILFFAAIAALFPLAI
                     LVVMALLRAKAPNPTKLSPYECGIASATDAHDHRFSVRYYLIAVLFVVFDVETVFLFP
                     WAVMYDKLLLFGLIEMAVFLYILVLGYVYVWKRGALSWA"
     gene            31132..31614
                     /locus_tag="PLP50_00580"
     CDS             31132..31614
                     /locus_tag="PLP50_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPA50067.1"
                     /translation="MGLIKDLPDNVITTSVDAVIGWGRKNSLWPLQFGLACCAIEMMA
                     VTDSRHDMARFGAEVFRGSPRQADLMLVAGTVTEKMAPIVRRLYDQMADPKWVIAMGS
                     CATCGGPYRTYAVTQGVDRLIPVDVYVPGCPPRPEALLYGLMELQKKIQKSRTLRRAS
                     "
     gene            31628..32368
                     /locus_tag="PLP50_00585"
     CDS             31628..32368
                     /locus_tag="PLP50_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPA50068.1"
                     /translation="MSEDKTISPETPAPEPVSKAPETPAPAAPEPRPVPAAAPAPAAA
                     AAAAPKPAVPAVPAAPPKPAGPKPTDASGKPLVAALAAAVPGSVLDAYEFVGETTVKV
                     DPGKLVEACRHLKDAEGFTYLVDLTAVDWKERDPRFDVLLLLHSFSRGQERLRLKAAV
                     GESCPSVTGVWLTANWMEREVFDMYGVRFDGHPDLRRILTWDGFQGHPLRKDFPVEGI
                     DTGAAIYPERYPEGGGPGPKDPNRKVLS"
     gene            32440..33528
                     /locus_tag="PLP50_00590"
     CDS             32440..33528
                     /locus_tag="PLP50_00590"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPA50069.1"
                     /translation="MELNFGPQHPSTHGVLRLVLKVDGERIVSARPDIGYLHRGTEKL
                     FETETFPMVVPHTDRMDYVASATNNHAYVLVVEKLLGVEVPKRAQYIRVLLDEMQRIS
                     SHVLWLATAALDLGAITPFFYTFREREVILDLFEEYCGARLTLNCMRIGGLPVDLTPG
                     WLEKVRAFTEPFEKNVDEYEELLTENRIWKQRTIGIGVLDEKRCIEWGMTGPVLRAAG
                     KAWDIRRAIPYEVYPELEFDIPVGKNADTYDRYLVRVAELRQSRRIVLQCLDWLEKNP
                     GTEIRGKVPRVIKPPAGEAYAAVEAPKGELGFHLVASGGNKPYRLHVRPPSFINLQAL
                     PEMAQGHLVGDLVAVIGTIDIVLGEVDR"
     gene            33536..34702
                     /gene="nuoH"
                     /locus_tag="PLP50_00595"
     CDS             33536..34702
                     /gene="nuoH"
                     /locus_tag="PLP50_00595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPA50070.1"
                     /translation="MSESLVHYVLVPFVKGLLTVILVATAAGILTWIERRGLGRLQIR
                     KGPNRVGPAGFFQWIADVVKLVFKEDVVPSRAEKVIHLLGPLLLFTPAVVVLGLVPWG
                     PELTLFGVTTPLHAAADLNVALLLILAVSSIGVYGIILAGWSSNSKYPLLGGLRSAAQ
                     LISYEVPMGFAIVAAVLMAGSLSMVEIIEAQKEAGIWFVFPGLVAFFLYFVSGVAETN
                     RTPFDLPEAESELVGGFHTEYSGFRWAIFFMSEYGNMISIGAIATTLFFGGWLRPFPN
                     VAWLSFLDVIPPFFWFAGKVFLFLLVYIWFRATFPRYRFDQLMALGWKALLPISLANL
                     FLVALAALWGAAGLKVLGAVLVLLVAGGLVAAWRRPARASVTTRMAAEKVEVRA"
     gene            34699..35196
                     /locus_tag="PLP50_00600"
     CDS             34699..35196
                     /locus_tag="PLP50_00600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPA50071.1"
                     /translation="MSFLDRVLLLDIFAGLRVTARHAGRKADTIEYPEQSKEPSERFR
                     GMFRLDEERCIKCTLCAIECPINIIYIDWHTEKTPEGKAEKKLDRFDLDMKRCMFCGL
                     CEEACPTNPRSIWLATKTYELGVYERDEALYLDKSKLTKWDGNVKPYPGVDPDKAPVN
                     PAKEK"
     gene            35198..35752
                     /locus_tag="PLP50_00605"
     CDS             35198..35752
                     /locus_tag="PLP50_00605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPA50072.1"
                     /translation="MEPGLFFFWLFSAVVVVGAVGVVVMRQPIHSALFLLLSFLGVAC
                     LYVQARAELVAAVQVLVYAGGVMVLFLYTIMLVNVKRQRTERYLHRQAVPALGFGFLF
                     LVVVGGVVVNEARALAGKAPAADPAALATTVLGSETVLGNTQAVGWVLYRDYLLPFEI
                     ASLFLLVAMIGAVVLGKRTMERFD"
     gene            35753..36055
                     /gene="nuoK"
                     /locus_tag="PLP50_00610"
     CDS             35753..36055
                     /gene="nuoK"
                     /locus_tag="PLP50_00610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPA50073.1"
                     /translation="MITLAHFVGLSFVVFTIGVVGVLVRRNLLTVLMSIELMLNAVNI
                     NLVAFSRQLGDLTGQVFSVFVIIVAAGEAAIGLAIVLSLYRLKSSANLEDAAEMRG"
     gene            36060..38294
                     /gene="nuoL"
                     /locus_tag="PLP50_00615"
     CDS             36060..38294
                     /gene="nuoL"
                     /locus_tag="PLP50_00615"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPA50074.1"
                     /translation="MSAADFLPWIPVFPLVGFLVNGLVYLVAHSKLGGKDAPLGAHGG
                     GHGEAHAAHAAGPDDHGHGGHVDIPFKAVHTWVGTLASGLSFVFALLVILSWWKETGG
                     HREVVTTLWTWIPMGTNAGWFGAPDFTVDVAFRLDPLSALMLGFVTLIGTLIHVYSVG
                     YMGHDEGYGRFFAYLNLFMFSMLTLVLGANLAILFVGWEGVGLCSYLLIGYYHTKDFA
                     ADAGKKAFVMNRIGDWGFLMGLFACVAMFGTVDFNGMFSAVSTNPAIYAAGGIVTFTC
                     LALFVGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARCNVFFRVSH
                     EWALKTLGEGATVWGLLTHDASFVVAVVGGVTAFVAATIGLAQTDIKKVLAYSTVSQL
                     GYMFLGCGVLAFGAGMFHVFTHAWFKACLFLGSGSVIHALSGEQEMTAMGGLRSKIPH
                     TYRTMLIATLAIAGVPGLAGFFSKDLILGETFIQGHPFLWALAALTAGLTAFYMFRLV
                     KMTFFGEYRGPKETWEHVHESPATMTVPLWILAIGAVLVGWWGIPNFIVPGGNRFEAF
                     LHPAFPVLPPDWTPHPHHASHALEYGLMFGSIAIAGLGILLATTFFYKGSAPWVLPAK
                     VVAAFPNAYRWVKNKYFVDEAYETVFVNGLVKKGGRLLWEVDARVVDGLVNGVRHLTV
                     GFSHVSSWFDKTFVDGAVNGVGDTFQAAWKGYGKLQSGQTQGYAFVMAAGAFALVCIY
                     IIFA"
     gene            38311..39948
                     /locus_tag="PLP50_00620"
     CDS             38311..39948
                     /locus_tag="PLP50_00620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPA50075.1"
                     /translation="MNGLLSLLILLPTAGALAILFLPREKTGPIKALATAVAGLTFFA
                     SLPLWFARGFRESAGFLFREDLSWIPSLGVRYSLGVDGIAALLILLTTLLSVISIYAS
                     YTAVKTREKEYYIFLLLLETGMIGVFCALDFFLFYVFWEVMLVPMYFLIGVWGGPRKL
                     YAAIKFFLYTLFGSVIMLVGIIALYFFNTTGFLWWKGLGNAPTFDILHFHAIAPQIPP
                     DLQLWLFWAFFLGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFS
                     LPIFPSAASTRWVQLTVVVLAIVGILYGSLVAMVQKDIKKLVAYSSVAHLGFVMLGFF
                     ALNNAGMQGGVLQSINHGLSTGGLFLLVGIIYERRHTRQIADFGGLSQVMPIYATLFM
                     IIALASLGLPLLNGFVGEFTILVGAFLADWKWALFAGVGVILGAAYLLWLYQRVFFGE
                     LKHEENKGLQDVNLREVLTLAPLIVCCVWIGVYPKPFFAMTAPASAKIVAALDAAKGA
                     PPALAAALSAAPTAPVPSATPVVPAGAPLPESAPRGN"
     gene            39952..41430
                     /locus_tag="PLP50_00625"
     CDS             39952..41430
                     /locus_tag="PLP50_00625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPA50076.1"
                     /translation="MALPFPLSDLLVILPELILSELALGILLWAAVVKRERERALAWA
                     SLASLAIAAALLPVAASFAGELPRSAFGGMIALDGFSIFFKALTLLAAAVTILFSLRF
                     VGTSPYPGGEYYALLLLTTVGMMFMASGTHLVSIYIALELMALSQYVLAGYFKQEAKS
                     VEAAAKYFVLGAFSSGVLLYGLSLVYGATGTLSLAGVSSALEAAPRSTLLMVGVVLVA
                     CGMLFKIASAPFHVWAPDVYEGAPTPVSAFFAVGPKLAAYAIFARVFFDGFGPRSDDW
                     APVLAASAALTMVVGNVGALMQTNVKRLLGYSSIGHAGYALLGLLAYRAGGAAGQPEA
                     FGLWAILFYLTAYLLMTFGAFGLVVLLEAKGYATESVDDFNGLWKRNPLAAGAMVVFL
                     LSLAGIPPTAGFVGKYLLFSAALKAGWPALVVLGVLMSAVSLFYYFRIVRAMYLVDGE
                     GEVRWREEPAVAVAVGLCFAGTLAFGVLPQPLIALAKSCLLP"
     gene            41438..41713
                     /locus_tag="PLP50_00630"
     CDS             41438..41713
                     /locus_tag="PLP50_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPA50077.1"
                     /translation="MPEPRREPDPILRLLGDVLSFGWVLPACLAAGGGLGWLVDRWLG
                     SFPAATAVLGLLGFAGGIRQLLREADRLEGRRKDTPGSGPGPAGDGT"
     gene            41710..42135
                     /locus_tag="PLP50_00635"
     CDS             41710..42135
                     /locus_tag="PLP50_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50078.1"
                     /translation="MSLALLPQERRVFRVAAWAALLAALALGALGRFREAAALTAGAA
                     VAIVSGLWLSDVAGRLLVPRPRASTRLDWKFALRALLRYAFLGLAAYGSVRLFPDEVP
                     WLLGGLSAVVLGVLADGFRGGAAGRSTPEEEEEGTTPRE"
     gene            42137..42934
                     /gene="atpB"
                     /locus_tag="PLP50_00640"
     CDS             42137..42934
                     /gene="atpB"
                     /locus_tag="PLP50_00640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPA50079.1"
                     /translation="MQHHHSILFGPVNALLTAIFGSPENAPSWWLHGVTIGGVHVAGV
                     APVDHATGAVLGWIPDHVIMALVVLLVFAVVLPVLARRFREGVPGGAQNVLEVFVDFL
                     RGLIKENIPHTGEKYLPVVGAFFFFITVSNLVGQLFFLQPPTSSLNTTFALSLSCLVF
                     FNAAGIKAHGVFGYLKTFAGPSAALAILVFPIEMIGNFARALSLAMRLFGNIYGEHTA
                     TNQFVELVPLLVPMPMNALGIFGSFLQAYVFTLLTTVYIGGAIAHEH"
     gene            42972..43301
                     /locus_tag="PLP50_00645"
     CDS             42972..43301
                     /locus_tag="PLP50_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPA50080.1"
                     /translation="MIKKLLPAVAVAGLAFLLPNLAFAQEAAEAAKATSGGAGWGHLS
                     AALVLAIGATAGATAQGRTAVAACDGMARNPGAAGQIRGIAFLGLVLIESLVIYTLVI
                     AFLLQGK"
     gene            43411..44073
                     /locus_tag="PLP50_00650"
     CDS             43411..44073
                     /locus_tag="PLP50_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50081.1"
                     /translation="MALFGFRRDRAPNAWDLRSGELPYADALFGTALRLTRNRQDAED
                     LLQETYLRAFAHYDSFREGTNLKAWLFRILKNGFINRYRQQKAGPREVDLERGGATFE
                     SALEEVVPPAATPEDELLGRSLDGDVARALTSLPEDFRLVVELVDLQDFSYREVADIL
                     EIPLGTVMSRLYRGRRLLEEALLAYGKRRGYFGATASPARSRRAAGEAAGAAEYPPRR
                     RR"
     gene            44106..44789
                     /locus_tag="PLP50_00655"
     CDS             44106..44789
                     /locus_tag="PLP50_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPA50082.1"
                     /translation="MDCETASPLIEALCDDELDVATAARLLAHLDTCAHCAALRRDAE
                     TRNAALREARPDDQIPEAVRTRLARAVAQAPGPRRTRRRGFLAVAAAAAGAAAVGAVL
                     FVRSSPPPAPTLVEASEVRELTGEVFCLRCALARLFPETPVLDPRHLPVLRTDDGEVV
                     TLLDGAVTEAVLARKGCSGRRVALTVRLFPRQELAEVLEARELGPASGPVPGEVVATA
                     VRRSSSSPP"
     gene            44814..46541
                     /locus_tag="PLP50_00660"
     CDS             44814..46541
                     /locus_tag="PLP50_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPA50083.1"
                     /translation="MAHEEPDRFIPILSLVAGLAAFVAGGAASVSAAWAAAGFGNPFR
                     QTLVLDLGLAASAVAGLLAGAAVAVFLQRPAARLGRTLAAARQMAEGDLASRAPEGRD
                     LAGRIGRFLNAVAGRGAHLLSSVRREHGQLNRQVAVLRAASIRNRERAAQERVRLSSA
                     TAAVGGLDGTIRAIAESVETLAAGSEETAAAVAEVDGSLTQLLARSEGLLADSTDGAG
                     AAASLAEGAAVLDATLSDLARRADDLAEGSTQAEKAHGAVLASAREAAEQAAHAATEA
                     RSGRAVVEEARASVAAIRLSSGTVRDAVGRVEARSREVGRILEMLEDIARQTNLLALN
                     ASFLATRAGEHGRGFAVVAAEIRRLSERTSEGARGIGGHVAGMREEVDAARAAAEEET
                     RLVTRGVETATRAGEALAGIEAAAARAEEAVAAIREVSRAQAATVAASVASVGEMREG
                     LRALAEEGRRNTREAERIRELVGRIRELAGFVERTVQEQKGAAGQIAVAAERSLGLMR
                     DIQDAVGRQTADSHRILGLLSEVEAMSLDTLASAASVDDTTAALETLAGSLEDEVGRF
                     RVGAELQAT"
     gene            46538..47017
                     /locus_tag="PLP50_00665"
     CDS             46538..47017
                     /locus_tag="PLP50_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPA50084.1"
                     /translation="MTLRRVVAFRAGGVACAADPADVERILRPASLLPAPGAPSWIAG
                     LVSGPRGLLAVVDVGRRLFPGRDTPGPRDLLAGTFDGIPAALGAEVVEGILTVDGAEG
                     LPPEGLAPAVADCLKGVVVAGGERRVLLDVRRLVAGEPARLAAQIASEATERRDQSS"
     gene            complement(46972..47520)
                     /locus_tag="PLP50_00670"
     CDS             complement(46972..47520)
                     /locus_tag="PLP50_00670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPA50085.1"
                     /translation="MLAGASVRLRPVKADDLDWLVAACGKPSAFGEFEPFFVGEAESL
                     RRRFEQDGLLSEDLTRLVIEDRAGRRVGLAGVDDVDLHSRVARITATILEPAERGKGL
                     GTDAHRALVSYLFLHRGLLRIEAFVAAGNVAARAVLKRLGFVEEGTLRSRVFAHGQRH
                     DVVVLGLLYEDWSRRSVASLAI"
     gene            complement(47520..49589)
                     /gene="argS"
                     /locus_tag="PLP50_00675"
     CDS             complement(47520..49589)
                     /gene="argS"
                     /locus_tag="PLP50_00675"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPA50086.1"
                     /translation="MIPGLKNHVEAAVKDACRRLWDVEPPRLVLETPPKVELGDLALP
                     IAFELAKVLRRPPRKIAEELAPALELPSIVARRSVEGGGYVNLFLDRPKALAAMLVAA
                     PEAAKASGKVIVEHTNINPNKAAHIGHLRNAVLGDVLVHLFRRLGKHVEVQNYLDDTG
                     VQVADVVVGLEHADELAEPTRAEELASEIREVLGAFPDDAPGTEPRRLGDLTWDLYAL
                     VTRSYAADPSLEERRKATLHAIEGAALGHELSGEARATAALASRLAEAVVRCHLKTME
                     RIGVSYDLLPRESDILGRRFWQKAFDLLKAAGATQLETEGKNAGCWVMKLEGAKEFEG
                     MEDADKVLVRSNGTVTYTGKDVAYQLWKLGDLGIDFEYEKAPYVNPFGEAEFFASDGT
                     SPLYRTRHDAEGADPSARGRFGGGDSVINVIDVRQSYPQKVVKEAVRRAGFPEGADRS
                     VHFAYEMVALTPASAELLGVTLSDEDRGRAYVEMSGRKGLGVKADDLVERLVEKAIEQ
                     ILDRQPGERPDLSRAEAIAIGALRVYMTRYSRNKVIAFDFDEALAFEGDTGPYLQYAA
                     VRTANIFRKLEEKGLPGLLDAEEAASVGALDAAHLDDGLWDVVRTCGRTQETFEKAAE
                     TFEVSLLVRHALAVGAAFHHLYHTHPILQAENDESRRARRAALQLVARTLEDVLGVLG
                     VPIPERM"
     gene            49793..50923
                     /locus_tag="PLP50_00680"
     CDS             49793..50923
                     /locus_tag="PLP50_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA50087.1"
                     /translation="MTRPPVRPWRSGALLVLVLGAFAGGCRTTDETPVLRLTPERWRE
                     AVAARGVDPGDVPMPMMVTPQIRAAAEQLAGPAGDDGERLRRLQAAILDRKSYAFEYD
                     TVATFTAAEAFAFRRGNCVSFTNLFIAFGRALGIPLQAALVFRRARSEKEGDLVMVYN
                     HMVAVLPKLRQLTVFDFYMTRDESRVDLRLIDDLAVAAISASNRGVEALRAGDLSRAR
                     TLLERATKLQRSLPDLHSNLGIVRWKQGDIEGAYEAFREGLDLDPHRPALLHNLAALY
                     IEQGRATEARAALAAANTRDASAYLFVVQGDLELAGGNFKQARTAYKRAHREGKDLVE
                     ALLGLARVERALGNEPAAQRALRKALKIRPDDPQVQALLGSP"
     gene            51021..52634
                     /locus_tag="PLP50_00685"
     CDS             51021..52634
                     /locus_tag="PLP50_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPA50088.1"
                     /translation="MRKLRLLATLVPLVALALPAAAQLDKFKDWESSPEFVYYATEDE
                     KKAWAGVTNDEEAQKFFNLFWGKRHPDYQKTAQNVFRTKFDALVAEADKRFTLGKKRG
                     ALTERGKVLILLGPPKAIASKVESADAGSAGAEGEAGSFLSRGNVVVIQFQYDKEQLP
                     EWAGMKSFRVNFTVDERAERETPDKTADIKKLWKKAIAAAVVNPKMTEPPVYKTREEY
                     EAEMKAAAEAAAEAAKGPVLSAPVRASLEALLAKEPQGGLSLFPLAYGDKATRLMVQL
                     YVPASVAPAGAAGAAPEGMKVALLVRGKDGKDAARREEPAVLQKVREDWFVDVALPVD
                     AGEYQVAALLLDASGAEKVSAHRPVTVTPLPSELAMSPLLLACTDIAADGAKAEEPFV
                     FSVRKFVARGGDVLEKTDGLAYVARVYNPAVDPATKKLHLKRSISIKPKNGSTIDVPQ
                     PADEPMIVPEQTGSATALVIDLAGSIVDVNLGDYFRPGEYVMTLKITDVISGKSVEAK
                     APFTLAAPPAPAKAPAAKAPAPKAPAAKK"
     gene            complement(52722..54356)
                     /locus_tag="PLP50_00690"
     CDS             complement(52722..54356)
                     /locus_tag="PLP50_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA50089.1"
                     /translation="MFARTASLALLAFALVLSPSPAMRGEEPPQEPLPVGVRAAVEVR
                     ALDLDVVATKDGRPVTDLTRDEFVVRIDKKVVPIDYFARVEAGTVHGPDLATASPDLV
                     LDTLRNDAGETYVPRQFLVYFDDERLLPQDRKPVLEGLRDFVMRLSPSDRASIVSYNG
                     ATRVLVPFTSSKEDLLGGLSKLEAVAPRGLALESEYQRTVREIRISPRSRASLIRNWS
                     TEAAHRERWALRELARSVSALAARSGKRTLLLVTNGYEWHPGRALALAYGPRLVSQFA
                     EDVGEEIEKVLAEANGAGITIQVFDAKGLGAGADASERSNPAISPFYRAQGFRDSMVT
                     LATGTGGTLVENRNEFRADLDRVYRESSGYYSVGVTLAGLAGAGERKVEVRTTRPGVV
                     VRARTHFVPRSADEAAVDRIEMALLTPGVQGDFPAALRIGSLSPAGGLARRTGPYEVE
                     FSLADLTFRDEGDRKTAVVEITLAAIQDTGARSDVTPEKKTLSIPVSEWERASSQPFV
                     YRGQVKSGKGNIRFVAGVRDVATGRMALASADVRVD"
     gene            complement(54421..55857)
                     /locus_tag="PLP50_00695"
     CDS             complement(54421..55857)
                     /locus_tag="PLP50_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA50090.1"
                     /translation="MSKSLSGTLDTFALADLLQWIEINRLSGRLTISRGEDRRMIDVK
                     DGAIVFVSSVRPEERLGTFLVARGVLPEPVVYELLADSFLTGRSLTRLVFETGLLSRE
                     ALAGAVEELALKVLLDLFHWRGARFVYDPTVVTEDLLRIQLSLRGQLLALEGARAVDD
                     TARIRAVRSEDLEEPEAEGDELSARRVARSFFSVVEGLDLESPPPSVWQERFELFGRF
                     SEKVAASLRKPFRPFPLFEDTVERCRAALASGTEGEETFRLAATDPFLTVDLLFVGNA
                     LRTPATGLLSTVDGALSAIGPGALRRTLELLTAPGVAVVSGRDRLERALRSAAVSTAV
                     AASHVAPSLGEDPGLAWSLALLEPVGGYELLKLLLAEDFDPGPFRAATLDWYRATSGR
                     ALARKLNLPVAFADVLGSDGQVSSRSSVAEQLIFFAKQMVAAEQVGREWTSDDPELAD
                     RYASLAVDEALADRIGRDASALRELLSL"
     gene            56010..57596
                     /locus_tag="PLP50_00700"
     CDS             56010..57596
                     /locus_tag="PLP50_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPA50091.1"
                     /translation="MSTTKTDTVTTSGIPVRPSYAPGDVPQGWDPGVPGAFPYTRGAM
                     AGGYRKKLWTMRQYAGFSTARESNARYRYLLAQGQTGLSVAFDLPTQIGFDSDAALAR
                     GEVGRVGVAIDSIEDMETLLDGIPLGEVSISMTINATASILLGFLLAVARRRGTEWKA
                     LSGTIQNDILKEYAARGTYAFPPKPSMRLITDIFGFCAENVPRWNTISISGYHIREAG
                     STAVQEVAFTLADGVEYLSAAVRAGLDAKAIAPRLTFFFNVHNDFLEEIAKFRAARTL
                     WARLLKDRFGIDDPRAQALRFHTQTAGSTLTAQQPDVNVVRVAIQALAAVLGGTQSLH
                     TNSRDEALALPTTEAARLALRTQQVIAYETGVARVADPLGGSWAIEALTAEIVEKASA
                     LIGRIDEMGGALAAIEAGYFQREIQEAAYQAQKAIESKGQVVVGVNEFVLEEENPIPT
                     MSIDPKVETEQVERLAAFRARRDGAKVERLRADLVAAAKEGRNLMPPIVEAVDGNVTL
                     GEVVESLKSVFGDHKDTVSL"
     gene            complement(57953..58546)
                     /locus_tag="PLP50_00705"
     CDS             complement(57953..58546)
                     /locus_tag="PLP50_00705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPA50092.1"
                     /translation="MLPGSGSIEVICGSMFSGKSEELIRRVTRAKIARRRVQVFKPKL
                     DDRFSEVEVVSHTGMRLTALSVATSAEVLARTEDRTEVVGIDEAQFFDAGIVEVASRL
                     ADLGKRVVVAGLDQDYLGRPFEPMPTLMALAEEVVKTRAICILCGGPASRTQRLVASK
                     ERVVVGAAGMYEARCRRCFDPGVVEVDGVLPFPEENA"
     gene            complement(58546..59280)
                     /gene="nth"
                     /locus_tag="PLP50_00710"
     CDS             complement(58546..59280)
                     /gene="nth"
                     /locus_tag="PLP50_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPA50093.1"
                     /translation="MPVRPKPKPAPAPAPEVLARLRAAFPGADCSLEHADAFQLLVST
                     ILSAQCTDARVNAVTPALFRALPTAAAMAAAGPELEELIRSTGFFNAKARSLRGACRA
                     IVEQHGGEVPRTMEELNALPGVGRKTANVVLGNAFGAPDGVVVDTHVGRLARRLGWSG
                     ATDPEKVERDLNALLPREEWVFAGHALILHGRRTCTSRSPRCSGCLLEEICPKVGVTS
                     ATKPGVDRQGTPGSRKPGAARAGRRR"
     gene            59357..61033
                     /locus_tag="PLP50_00715"
     CDS             59357..61033
                     /locus_tag="PLP50_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPA50094.1"
                     /translation="MSIRRPLLLLLLALVLAVPALAAERVTLTVLQTSDLHANVLPWN
                     YARGEAADWGLARVATRVREIRKSSKHVLLLDGGDTIQGAPAGWLEARRPKGEPHFMA
                     AAMSALRYDAMAVGNHEFNFGLEVLRRAQKDSSFPWLSANTRHAAGGGPAFAEYVVKT
                     LGGVRVGVLGLTTPNIPGWEPEANRPGLEWENPVESAARLVPLLRQRERCDVVVVLFH
                     SGLEIDPATGEANGTDYENHVVRLAKTVPGIDLILTGHSHRRLPLTRVHGVPVTQPGR
                     WGEVLARVDLVVERAKGIARVVEARGELLPSDGTVSVDPEIAAIARPHHERALAYLDE
                     PIATAAGPFPADRARLEDSALLDLINETQLAATGAQLSMTSLLPYRHDGWKEGPVTVR
                     DVYSIYPFENQLVVVEVDGKRLRALLEHAASFYESAEWKDGRLLLTPRAGMRPYNFDV
                     VQGVSYRIDPTAPAGRRVREVRYRGREVRDEDVFTLAVNSYRAQGAGGYAALRGTRVV
                     RAYNDEIRDLLVERLRSAGTIHPVTDRNWVLAPETSWAPPPAAADAPAPR"
     gene            complement(61124..63766)
                     /locus_tag="PLP50_00720"
     CDS             complement(61124..63766)
                     /locus_tag="PLP50_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50095.1"
                     /translation="MTRLFRMAALAAGVAATALPLFAQTPTPTPTPTPTATPTPTPIP
                     STTIEASVASLGIPNGGRYDASNMILEADGTIWTASANENVLARISADEKKVRKWTMP
                     RDAAPSHLLKEPDGKLWVAQLGGFQVSLFDPQTAELTEWADGARRPTAFVRKGDGTLW
                     LPETNGTLANFDPATGTFVYWRSTDAANPISSLTYPFLDADGSLWSADFLRGALLRFA
                     PDGSKVTRWALPDATAQPSKIIRGPDGHLWFSFYGTAQLARFDPATRELKRYAIGVFS
                     QPFDMKVYKDRIVYTEQLIGEVGVFDPFGSTPAATDTLEEKEIVLTQTTATVAPTKTV
                     LETVELDVAPADAAAVTGSGLPGLARYTAVSGVPYALVLDETRKRFLVGATAEIAEVL
                     PPIPASLDDHFFPAASSIGGRGGVRWATQVVTWNRGSADTAGVRVDATVNERLLPTDW
                     IIGLSPTANLTIGPGKIVSQADPIGEEMKGPDTSGALRLTSGATTTRFGDVHSWIRVY
                     RARDDGGTYGFARNYVKGAEALATGETGFLFTPPDGGASRVNAGLLVVEAAEGTVSVV
                     SAAGTPLSAPFAFRWPAGYHLQASTVFSAFGIPPSPSARIVYTVAKGRVLPFGTSIDA
                     VSSDPIDLPFFGPRATATNQWVFGVERGGGPLGPTSRTDLQLFNGAAGDASVSLVLRA
                     ARLAGSPGAAPPARAVSLTVPAGKVVALDDVVRETFGVDVFAGSLDVVSDPPVHAFAR
                     VTAADASGGRHGYGMPALRADSTAAAGSRAVFIQAADAGWDVMESELQLTNPTDTAGE
                     VTLRAFDTEGVAAGEPVTLPLGPKESVRVPAAFYSLAGFGTPIGRIDVSPVAGSQPVF
                     ALLVRRDQKTGDADAVVPYVVPAS"
     gene            63837..64967
                     /gene="amrS"
                     /locus_tag="PLP50_00725"
     CDS             63837..64967
                     /gene="amrS"
                     /locus_tag="PLP50_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPA50096.1"
                     /translation="MAPADSVFERLAGLTAKATLVREETGKLRCLACGHRCLLAEGGQ
                     GVCRVRFVRDGELRAPWGYVASVAADPIEKKPFFHVLPGSTALSFGMLGCDLRCPYCQ
                     NWEISQTLRDPNADDWAHPSPATPEGIVAAALEAGAQVVTSTYNEPLITAEWAAAVFA
                     EARRAGLLTSFVSNGHATPEVLDFLRPLVDAYKVDLKGMREAAYREVGGRLSVVLDAL
                     AGLVARGFWVEVVTLLVPGRNDSDEELRDAARFLATLSPDLPWHVTAFHPDYRLGDVR
                     ATPAATLLRAVDIGRGEGLRHVYAGNLPGGVGDAETTVCPGCSERLVERRGFRVVSRR
                     IDAEGRCPRCERLIPGIWSPGGRVVLRAPGGRPAEGEDVPTG"
     gene            65208..66602
                     /gene="rho"
                     /locus_tag="PLP50_00730"
     CDS             65208..66602
                     /gene="rho"
                     /locus_tag="PLP50_00730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPA50097.1"
                     /translation="MDEFDPKEPVEADRPSVETTVVEELPEGDDDAEALDEGDGETEA
                     VASEGGSVPGEPSRKRRRRRRRRGRSKAAGSEGAPGAESSGEGAPRRELAPQAAPPVT
                     VRGLVKLHGDHSGLVVSPETFFAEKGDPFLPKYLVEGEDLEDGLLIEAEALVRGPKGP
                     MVQKILSIEGMTPAEYRSKYVQFHKGISIDPNRRLRMETSADENSGRVLDLVVPLGRG
                     QRCLIVAPPKAGKTFLLKTMANAIAKNDPDVIIFMLLVDERPEEVTDMQRGITGQVIA
                     SSADMNAAHHIAVAEATFERAKRLVEIGKDVVIFCDSLTRMSRAYNNEQRGSGKILSG
                     GIDARTMEKPRRFFGGARNHEDGGSLTIIATCLVDTGSRMDDVIFEEFKGTGNCEIIL
                     DRGLFDRRVFPCINIPASGTRKEEKLYTAEELPKIHKLRRALSSVKPMDAMELLLSKL
                     TRTKTNAEFLKSIA"
     gene            complement(66758..67567)
                     /locus_tag="PLP50_00735"
     CDS             complement(66758..67567)
                     /locus_tag="PLP50_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA50098.1"
                     /translation="MPFAGVRDQRFYYEVGGEGDLVILLHGALANADLMEAPATGLAS
                     GFRALRFDRRGCGRTTPPTEGPVPLPEEADDVLGILDWFSVEKAHFLAHDEGAEVALE
                     FALRNPGRTGSIALLAPSLEGFSSSPEAAAAAADLRAALRSDAPKALAEKLLPSPIFD
                     AAREREGIFERIADIFNRFPQSPGRFERLPRAGAALVGRLGAVSARTFVLVGERDEED
                     RIRCARTIAAGIPGAELLVFPDTSRFLHVEESRAIMRRLTDFFLPEPEIEP"
     gene            complement(67616..69364)
                     /locus_tag="PLP50_00740"
     CDS             complement(67616..69364)
                     /locus_tag="PLP50_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA50099.1"
                     /translation="MSSSPPGTGDYLSDVFFTDLPLPHAVRAGIAACGFVRATPVQAS
                     TLPLLLAGKDVAAQAQTGTGKTAAYLVSILTRLVEAPSSANRKPGAPRALIVAPTREL
                     AVQIEHDAILLSQFVRPRIVTVFGGLDYVRQRSLLQAGCDILIGTPGRLLDYEGQGAT
                     SFGSVESLVIDECDRLFDMGFLPDLRRILRRCPPPRRRQSMMFSATLSWRVTELAWEH
                     MNEAVRVEIAPEHVTADRVVQSLFHVGNSEKLSLLVGLLQREGNATRTMLFVNTKRFA
                     ERLVERLERHGLRCGAISGDIPQAKRLKILADFKSGKLPILVATDVASRGLHIDGVTH
                     VINVDLPMDPEDYVHRIGRTARAGHSGRAISLACENYVQSLSSIEKLIGMKIPVEHPD
                     DALFVRRPAPLREPVRVSGGPRSAAPVAGDEEEDTKAPPPPRASRPRRERAAAPPRPE
                     PVPAAPAPRVEKPQAPPATAPEPFVPDIPVAPVAPVVRAEAVVLAEAAAPLAVARVEA
                     AKSGAPAEPAADLPGAVEPPPGDVAPTDFWTCAAFELEIPEGGFGVSASPAAPGGASR
                     RKRHRRRKPASAPSAS"
     gene            69440..70948
                     /locus_tag="PLP50_00745"
     CDS             69440..70948
                     /locus_tag="PLP50_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPA50100.1"
                     /translation="MSAGASLRIVSPSTGELLADLPAAGRAEVAAAVDAAREAAPAWA
                     ATPLPERVAALKRWRRAVVDDEELLRTLVRESGKPRHEAEGVELLYFCELTRFVAAAA
                     RRALAPERRGTLLFAVRRTDVHRVPVGVVGVIGPWNFPILNNAADALAPLAAGNTVVL
                     KPSEVTPLTSLRMEELWRKAGNPPGVFRVVAGRGDAGAALVDLVDGVMFTGAVETGRR
                     VAARCGERLVPCVTELGGKSPFVVLSGADLDRAAQSAAWSGFFHSGQACIRSERILVE
                     ERVADEFERLFLERVRALRQEVPAPDAAREHDLGAVTFPRQIEVVTRQLEDAVSKGAR
                     LLAGGRPRADLGGAFFEPTVLAGVTAEMAVFREETFGPLVPIVRVADADEALRLANDS
                     HLGLSASVWGPPRAARRLAERIEAGSVAVNGALVHYLVVSAPLGGAKASGVGVRHGVE
                     GIRAFTRSRAVSVPRALLAPLDRLLLSRLAFPYDSRVLGLLRRAVRLLYRRG"
     gene            70993..72132
                     /locus_tag="PLP50_00750"
     CDS             70993..72132
                     /locus_tag="PLP50_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50101.1"
                     /translation="MKATSRAVPALLLVMFAAPLLAQAPKPAPTAPAGGAIVGTNPQG
                     PPGSRPAEIQDVRVLGASPRDPWQMERLGVAAVQKNELGKAREFFEESWKAGELPTAP
                     YNLACIDAREGKADAAFRQLDRAIAAGFDDEATLAKDTDLASLRGRPEFARIVQGARR
                     NRLAGDEAVVKEGVFVSPGRPPKGILLLLHEVNSDPMSIAGPFVAEAKARDLFVAAPR
                     GPARSGRKRFGWGSPDRALEALKRTIAEAQTRAGGLPIPVAVVGAGRGGKLGLEIAAR
                     NPGIFSAVASIGGIFDPGPTGAAGVAGLRGVPVFLGVAKSAPPELLRAMQRGRENMRK
                     LGVKVRWGEWPGTGEGLPADAAAAAKDVLDAIRSPRPGAAGSSAK"
     gene            complement(72149..73924)
                     /locus_tag="PLP50_00755"
     CDS             complement(72149..73924)
                     /locus_tag="PLP50_00755"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPA50102.1"
                     /translation="MRRSLPVSLLVALLSGGAAVNAWSDHEGHERLARAGHPEGRAKR
                     ARALVSELARSRSGRGALSPEGAARGYDVLTYDLRFELDPAVVALEGSAVIRLAAIRP
                     GVSEVRLDLDDAMSVRGVERDGSPVTTFTAAGDVLTVPLTPPLGAEERTTLVVRWGGT
                     PLAGGALTFWTAPASGPAISSLAEPFDARTFWPCVDDPADKAVVTLSATVPSGYVAVS
                     AGRGASTSEPDGRVTWRWRLPQPISTYLVSVAVGRFEEIAVEYRTADGRTMPVVGWVV
                     PEHAAPNRARVASMVRHLEVLSSLFGDYPYLDTKYGIVEGSFSGGMEHPTITLIGASL
                     LGNASRDLTDLLVHELAHQWWGDEVTMRTWDDIWLNEGFATYAEVLYRERTSSFSPGL
                     LLASRYDDGLYSGQLGPAVVAPPSDPFRHTGAVYSKGAWVLHMLRRRIGDDAFFAAVR
                     EYRRRHERGNATRGDLRAVFEEVSGRDLKQHFDQWVETPYRPILRATFRNVSGGAEVN
                     VRQVQTHSVVHPRPGPGDVPHYVFPLTLRVFTANGGSKAATVEVTRADETFFVPLDGG
                     AAAVGVALDPDGDLLEIVEAVGPAL"
     gene            complement(73936..75303)
                     /gene="hemG"
                     /locus_tag="PLP50_00760"
     CDS             complement(73936..75303)
                     /gene="hemG"
                     /locus_tag="PLP50_00760"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPA50103.1"
                     /translation="MRRIVILGGGVTGLALGYLRNRCPAPGDDVLILEEGPSPGGTVR
                     TIREEGLVLEAGPVTLRTTPASDRLVEALGLEDDVLVADPRSPRWIVRGGRMRRVTPG
                     PSLLLGGALPLAARLRALAEPFVARRPADLADESVHDFFERRFGPGVARWGAEPLVSG
                     VWADDPRTLSVRSAFPALWDAEGRAGSVLRGLAGAPRPRSRRTISFRGGLGTLAERLA
                     EQGVRAGVRIELDAEIAGIEGPFDGEGNGGVWKVQIADGTVYPADTLVSTLDAPSFAE
                     ALGPRLPRSGARLATLPYSRLAVVLQAFRADSPDAAPRGFGLLAPRGEGLRSLGVLYL
                     SSLFPSRVPAGVALTTSFFGGALDPSALDLCETDLLRIAEAEVRRLHPDLGPRLHGRA
                     LKWPSALPRLPVGHHETLRRLDADLDDLNAGRERPRLVVTGTWRDGLGLGERIARAEE
                     LAPLL"
     gene            complement(75300..76298)
                     /gene="hemH"
                     /locus_tag="PLP50_00765"
     CDS             complement(75300..76298)
                     /gene="hemH"
                     /locus_tag="PLP50_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPA50104.1"
                     /translation="MAAGKTGILLVQLGGPTKREELKGFLYRLFADPEILGIPFAPLR
                     KAIAWTIATTREAESAKTYEKIGWSPIRCWTERSAMLLEAALAPTWPGEPPLVRAAMT
                     CSAPLVEDVLPDLRAAGVTRLFVLPLYPQYSVTTTRSTFARVDQALSRMGWSPEKAHA
                     PDSWYAEPRFLDAHAERILAAAATLPDPDPAATVLLYSAHSLPVSTVEKKKDPYPKQI
                     EECCALVDERLGRRFRSRLGYQSKLGPVAWLGPATGEVLARLASEGVKQVVVCPIAFV
                     SDHVETLYEIRMLFAEEARAAGITHYVAADGLNDHPAFVDALADVCRKALLAPGAR"
     gene            complement(76298..77728)
                     /gene="hemN"
                     /locus_tag="PLP50_00770"
     CDS             complement(76298..77728)
                     /gene="hemN"
                     /locus_tag="PLP50_00770"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPA50105.1"
                     /translation="MPAIPIRLQGETLPMTTELLSKYNVQGPRYTSYPTAPEWTDTIG
                     PADFEAACERANAAGRPISLYVHLPFCDEQCWFCGCFMKVVPKPDRQGEERGEIEGYL
                     ADLHREIDLLAARVDRSRPVVQVHWGGGTPTYLTPKQADALAEHLLAAFRPAPGAEIS
                     VEVDPRVTTREHLAVLRRHGWNRVSMGVQDFDPEVQELVNRVQPFEMTKALVGASREL
                     GYESLNLDLIYGLPGQKLAGFSRSVEQVLTLRPDRIAMYSYAHVPWLKKPQRVLAAHL
                     PEGTEKFAIFVSGIEKFSEAGYVYIGMDHFALPHDEIARAQAERTLWRNFQGYTTHAG
                     VDLYALGVSAIAQIDDCYVQNTKDYAAYHAACAEGRPSTIKGWRLTSEDHLRRTVITN
                     LLCHCVIDKREIARQFGLASFDETFAREVAALPAFVADGLVEPRTDEVRVTPLGRIFI
                     RNVAMLFDPYLLRRPADQPKVFSRTL"
     gene            77843..79705
                     /locus_tag="PLP50_00775"
     CDS             77843..79705
                     /locus_tag="PLP50_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPA50106.1"
                     /translation="MPEESRPPFSWVNVPPSEPVRSEAARGLLRALCDPGRLETAFQP
                     VFRARTGELEGCEALLRIPAGSGFSGPGEAFTVALAEGLGGDLEAAAIQRALRDAEPF
                     AGDRLVFLNLLATSLSDSRLGARWLVERAVAHGRVPSQLILELGQISRIVDFAALARA
                     LEPYREEGFRIALDDFGSGYTNLRMITDLAPDFVKLDRVYVENVFVHARKRILVESVV
                     SLCHRINCGVIAEGIETPDDLETCLGAGVDLLQGFLLARPGAPEEAFLAEDLTVEAAG
                     GHGRGEIAAAVSHGPTLAVEAPLAAADDLFRAESDLDVVPVLQRGRAVGLLARTRAET
                     FRFVSPGSGADTVAAAIADAPWDQIEEGTPVEEVAAFVARRPRARRFEPIVVVGPGRV
                     YRGLLRLDDLLGLFARLYVENGLESHPLTDLPGRGRLEAEVDRRLTRGASFALARLNI
                     LRLRAFNDRYGIRRGDRLLAHVAAVLRTLATEEPGSFLAHYGADDFGFLLSPERVVET
                     LRRAVQGIASTVGEFYDPEDLSAGGIAGHDARGGVILVAPAALVAGVASWGGGGAPTL
                     RTVVTAAEAALREARRGGTQPVVRTVGPAAAPAATPERSEQPPRTQASLPKPFR"
     gene            complement(79713..80768)
                     /gene="hemE"
                     /locus_tag="PLP50_00780"
     CDS             complement(79713..80768)
                     /gene="hemE"
                     /locus_tag="PLP50_00780"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPA50107.1"
                     /translation="MLTSRAMSVPGPSRQRFVDAAFGRPVDVPPVWLMRQAGRYLPEY
                     REVRKRLAFLDLCADVPSAVEVSMQPFRRFGMDGVILFSDILIPLPPMGIEVKLDEGG
                     PKLPTPVRTAAQVEALRGFDPTVGTGFVPAILRELKKEVAGRAAVIGFCGAPWTLATY
                     AVEGGGSKSFQHTKTMMWREPKLLEALLGKLADAVGDYLAAQVEAGADVVQVFDSWSG
                     ELSKADYDRFARPATERLLARLPRRGEVPVIHFASGSAHLIDSYAEMASDVISVDWKL
                     PLDEARRRACGKALQGNVDPGVLLGSPEDVKAAVRTARAAAGEGGHVVNLGHGILPPT
                     PPENVAAFVEAARESRA"
     gene            80779..82212
                     /gene="murF"
                     /locus_tag="PLP50_00785"
     CDS             80779..82212
                     /gene="murF"
                     /locus_tag="PLP50_00785"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPA50108.1"
                     /translation="MATPIPQNTARFTPAEVARATGGVLVRDGRPLAGVSTDTRTLAP
                     GALYVALRGETFDGHRFLPQATAAGAGGVLISTAGDLPPNGAVIRVPDTRVALGDLAR
                     FHRDRWASTDGRKVLAVGGSAGKTTTTRAVAALLDVLRPGEVQTTPGNLNNDVGLPMT
                     LLLLDEHHHLAAVEVGTSHRGEIARLARIARPDAALITLIAPEHTEGIGTLEDVADEE
                     GDLFAALGADGFAIGNGDDPRVAAQIARAPGERRILYGFGESCDVRALAREVRGLDGS
                     VLQVAREGAEAVAYEVPLAGEAGAYAALAALAAVRGLLGVDAPPEALREALLRVASVK
                     DGRFAPETLADGTVLLDDTYNSNTGSALSSLKTARELADALGRRLVLVLGEMRELGEL
                     APAEHDVVAAAAVEARPAALVAVNGEAQRFARAAEAAGIPAAFFATGEAAAPRVVEAV
                     RPGDVVLVKASRGVRLETVAAALRVAR"
     gene            82235..82750
                     /gene="purE"
                     /locus_tag="PLP50_00790"
     CDS             82235..82750
                     /gene="purE"
                     /locus_tag="PLP50_00790"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPA50109.1"
                     /translation="MSTPLVGIVMGSASDHETMAKAEEVLRDFGVPYETSVVSAHRMP
                     EEMFDYAKGAAARGLKVIIAGAGGAAHLPGMVSALTTLPVLGVPVRSRALSGLDSLLS
                     IVQMPGGVPTATFAIGSAGATNAGLFAVKILALSDPQLAAKLETHRELLREKARAGNE
                     EVRRVRGGTVP"
     gene            82747..83940
                     /locus_tag="PLP50_00795"
     CDS             82747..83940
                     /locus_tag="PLP50_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPA50110.1"
                     /translation="MTTVGVLGGGQLGAMLASSLQDLGADVHVFDPDPNAPALRRAAR
                     AVTAPWSDSARLQDLFDACDVVTYEFENVETEGLARVSGLAKLAPSLDVLRTTQDRAF
                     EKEFFRSHGLPHVAFAAGRDADDLRAAARSFGLPAILKTARGGYDGKGQWKLASEAEL
                     EALLASPARAALESGGWVLEEPIEIALEASAIVARERNGDEVVFPLFENVHRDHVLDV
                     TLVPARLPEDLEAEAKSIALAAARALGVTGLLTTELFVGRSTRHRPAEVRVFTNELAP
                     RPHNSGHVTRKACTYSQFDALARVLLGIPLVPPRLVSAGAFCMANLLGEVWQAQGTEG
                     RLDLSAWEGSPDLLEVVLYGKSGVATKRKMGHLVARGAGADEAIAAARGFRRALSRRG
                     PQGSL"
     gene            83953..84795
                     /gene="ccsA"
                     /locus_tag="PLP50_00800"
     CDS             83953..84795
                     /gene="ccsA"
                     /locus_tag="PLP50_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPA50111.1"
                     /translation="MSGTGLSNLLFGLLALVCYGGATLLYVTWLRHPRVSAGRLATLL
                     AAAGAVFNFVFLYVRSLALKTVPYSDLLGSMALFGLFLAAMSLILETRHHDRSLGAFL
                     MPVSLFFLLHSFLLPGGTPPPPEMRGSIFAFHVTLNMLSYSAFAVACALSLLYLVLGR
                     RLRSKRHVLNGPVSRLPSLSYLERAARTSIGVGVVSLATGLTMGFYWAYRVWHEQHPD
                     WLLDAKVWGSVVTLAFYLWVFVRAHRGAAPGSTAKLSIAGFVLVLLSYTAVNLFFSKV
                     HSFA"
     gene            84795..86069
                     /gene="hemA"
                     /locus_tag="PLP50_00805"
     CDS             84795..86069
                     /gene="hemA"
                     /locus_tag="PLP50_00805"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPA50112.1"
                     /translation="MPPEIVFVGWSHQGADLDLRARLAFTPEKAREALEGLFRERILT
                     EGAVVSTCNRAEVYGVAEAPGSIDALAEFFSRFHGVEASVLREKAFSGQGEATVRHLF
                     RVASGLDSMVLGEAQILGQIREAHRRAAEAGTARAVTSRLFQSALECGKRVRTETTLG
                     ARPVSVPGIALSLVGRIFESVSDCKVLLLGAGETVELTARLLADDGVKQITFTNRSPE
                     KAKALAGCFHGWTVPWEERAKAFAEVDIVLSATGATEPVVTFDMLKPVLGRGRRRGPL
                     LILDVAVPRDIDPKVEEFSDVYRYDMDALGELACDNAKDRMADVPQAERIVEESTERF
                     LGWLAGLSHVDTLKTLRGRLEKARLDELERYSGKLSHLTAEDRAVVERMTETLIHKIL
                     HNPTIGLKEGDASERLTRAAVVRALFRLDDGE"
     gene            86203..>86350
                     /locus_tag="PLP50_00810"
     CDS             86203..>86350
                     /locus_tag="PLP50_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPA50113.1"
                     /translation="MSHGRPLRMGTRASLLARTQSGHVAAALTKATGRAVEEVLVRTE
                     GDQLS"
BASE COUNT        11712 a        30726 c        31307 g        12605 t
ORIGIN      
        1 agctccggac gttgatgacc gggttgtcgg ggtcgttgtt gacgtcggcg accggagcga
       61 agacgacacg gatgccgagg gcgcgtgcgg cctgcgccgt cgcgcgggcc cgtcgcgcgg
      121 cgagtgcgag gtcacccgtc gccgcgaccg ccatcgccga cggcccgagg acgaggtcgg
      181 ggaatcgcat cccgggccct gcctcgaggt ccgcggcgac gaggagcggg agcttcgcct
      241 tcgcattcag ccggtgattc aggtcggcgg tctcgagcgg cgtcccgagg aaccagatga
      301 cgccgccgac cccctcctcc gagacgagac gggcgagctc gaggtactcg gggtcgtcct
      361 cggcgcggta gacgccgcgc gaggcgacca tcacgacctg cgcggccttc tcgcgcagcg
      421 tcagcgcggc gacggcgggg gcgcgccggg ggagggatcc ggctcgggcg gcgccggagg
      481 acccggggcc gggccggccc gtcgagcagg acgcgagcgt cccgaggagc gggaggagga
      541 ggaggagggc gagcgggccg ccggaggtcc gacggcccgc cgcgagaggt ctgtcggcgt
      601 gcgtcacggc ggccgaatca taggctcggc cgccgcggcg cggggctcac ggcacgatca
      661 tcggaacgaa gctcccgtcg tcgttcccct ggtcgttcag gacgccgtag gcgaggaact
      721 ggtcggaacc cgcgacgcgc cggatgcgcg cgtaaccctg ggtcacgccg aagcgtgcga
      781 gcgcggagtc gaactggagg aactgccccg gcgcgagcgt gcagggagag cactgcggct
      841 ccttcccgag ctcgctgccg ctcggcccga agtaggtcac ctcgagctgg agcggctcga
      901 cctgccctcc gagcgcgtgt ccgacggcga ggttcgcccg ggtgccacct tcctggccgc
      961 gggtgccggc ctgctggagg ccgtagaccc aggcttccgt gtcggctgcg tcgccgatcg
     1021 gcgtgtagcc gtaggcgagg ccgaggaggc cgccggctcg cgtcgtcgtc gtgcggacgc
     1081 cgaggaagcc gtcgctcgtc gggatcggcg cctgcgtgtc gctctggtcc tcccggaact
     1141 gcccgtcgag gaactgcgcg aagacgctcg cgacggtccc tgcgggagcg ctgaagccga
     1201 tgcgccggag ctccgcgacg acgttcggga cggtgaactg agagcgcgcg gggaggtcga
     1261 agtagcccca gtcggggacc ggggtcgtcc gcgggacgag ggcgaagagc ccggagatgt
     1321 cacggctcgt ccggttcgcg aacgtcatct cggaggagta gccggccgcc tcgaggacga
     1381 cggggacgag ggccgcggag gtccgggagg gcgtgtcgtt cgggaccatc gggacgaagg
     1441 cgccgtctcc gttcaggttg tcgttgagga cgccgtagca gacgaactgg tccgagcccg
     1501 acgtccgccg gacgaccgcg tagccgtacg tcaggccgcg cgaagcgagc ggcgtcgact
     1561 tctgctccca ctggaacgga gcgaggtcga ggaagtcctt cgtcgggtgc gcggcgcccg
     1621 tgtcgccgtt gtagtaggtc acctcgagcc ggacggcgct taagccgccc ccgccggcat
     1681 tgacgcacgc gaggttcgag cgggtcccgg cgcttccggc cgcggaggtc tgctggaggc
     1741 cgaagacgaa cgcctcgccc gaggcggctc gcccgagcgg cgtggcgggg aaggcgaggc
     1801 cgaagcggcc gatgaccccc ttcgcgcgga ggtccgccgt cgccggcgtc gtcacccgga
     1861 cctgcgcgcc gaactgaacg aggttgtcgg cgccgcgcac ctcgatccgc agagaggcgg
     1921 tcttgttccc ggccggcacg ctcatgccgg tcgaggcctg gaggaacgcg aagatgtccg
     1981 ggtggatctg ctgcccgggc ggcagcgagt aggtggacga cgcctcgaac gagcccttca
     2041 cccggaaggt gaggttgagc gtccggccgg agcggttcgt cagggtcagc tcggaggtga
     2101 agaaggctcc gccggcaccg ctcgacgtca ggacgatcgg caggaggtag gtgtaggcgt
     2161 ccccggtccc ggtcgagccg taggagacgg tcactcccct cgtgacggac cggctccccg
     2221 ccccgttggc ggcggtcagc gtgacggagt aggtgcccgg gtttccccag accttcgtcg
     2281 ggttctgggc cgtggaggtg gtcccgtccc cgaaagacca gctccaggtg gcggcccctc
     2341 cctgcgtccg gtcggagaag gagaccgact gcccgggctt gggctgcgcg ggggcgaagg
     2401 agaagtcggg cgtggggggc gaggtcggcg acggcgtgcc gccggcgggg ttgatctccc
     2461 acatcccgcg gccccaggtc gccgcgcgga ggcggctccc gtcgggcgag aagagaaggt
     2521 aggagaccca ggtcgcgggg aagtcgtcgc cgaaccggct ccacgactga cccttgttcg
     2581 tcgagcggaa tatcccgtac ttcgtccccg cgaacatcag gttcgggtcg gtcggggaga
     2641 agacgaggct gtcgacgggt gcgaagaagg tgaaggagcc cccggcgtac gtgatcggga
     2701 cgccgatccc cgagctggcc ggggaggcgg tctgctgctg ctggttgagg ccggcgatcc
     2761 gccagatgac gtgccggcct tgaccctgga cgacctccgt ggcgctctcg agcccgacgt
     2821 agagagcatt gccgtcggcg gggtcccacg cgatcgcgtt cacctggctg ccgatgttga
     2881 cgagcgatcc gttctggaag ccgttcaggg tgtagacgac gtagccggcc gccgtcccga
     2941 aggcgacgcg gttccggtcc gtcgggcgga tcgagatgga ggagcagctc gcccagccct
     3001 tctgcacgtt cggacagggt tgcgcggaga cgccgtcgga gcagaagtac tcggcgaaga
     3061 ccggctgcca gtcggacggg gtcgcggcgc cgttcgaggt ccggtagatc cggtcggtca
     3121 tggtgtagag gacccgcggg tcgaccgggt ccatcgcgag ccgggagcgg aacgaggtga
     3181 ccggcgggac ggagcactga tcctcggtct gcgcgtcggc gggacaggtc gtcaggcgga
     3241 acgactcttc gctctctcct ccgtcgtcgg cgcgggaggt gtagccgttg aagttcgtcg
     3301 cgtagacgat gttcgggttc tgggggttga cggcgcagtc gtagccgtcg ccgtagaaga
     3361 tccgcttcca ggcgctggtg ctgtcgccgg agaggcgcat cgtcccgttg tcctgcgtcc
     3421 ccgcgtagag ccggtacggg tcggtcgggt gctgcgcgag cgaggagaac atccgcgcga
     3481 cgacgccgtc gttcttccag atgaaggagc ggcctccgtt cgtggagcgg aagaggccgc
     3541 cgtcggtccc catccagaag ctcgaaaggc ccggctcgaa ggcgatgacg ttcacgtccg
     3601 cgtggcagaa ctgcgggtgc cgctcgggga gggtccactg cgagatctgg gtccaggaga
     3661 ggccgccgtc ctcggacctc caggtgtcga ggccccccgc gatgagccgg tcgcggttcg
     3721 tcgggtcgac ggcgaggccg aggaaggtcc agccctgcgt gccggaaatg agcggatagc
     3781 cctgccccgc gggggggccg agcgcgacgc cccagttctg cccgccgttg tcgctccgga
     3841 ggacgccgac ctgcgagtag ccgcgctgct ggtcgtagcc ggccacgagc cagtagatcc
     3901 ggttcgggtc ggacggggcg atggcgacga gggagcggat cgggatgccg ttgaagccga
     3961 cctgctgggc gatgtcctcg aacgccacct gccaggtgct gccggcgtcc gtcgagacgg
     4021 cgaggccacc ccagagcccg gcccagagga cgttcgggtt cgccgggctg cgggcgatgt
     4081 tgacgccctg acggcgccac tcgagcccct cgccggtcgc cgggaggcgg ttcgaccagg
     4141 tccggccgcc gtcggtcgag cggaagatcc cgttcgccgt ccccaggacg acgtcctccg
     4201 ggttcgacgg gtgaacgtcg aggtcgtagg agtagaccga cgggatgacg tcgaaggctc
     4261 ctccggccac ggagatggtg ttgaagctcc aggtcgagcc gccgtcgttc gagcggagcg
     4321 tcccgaggcc gatgacctgc gagccgctca gcggcgactg ctcgccggtc gccgcgtaga
     4381 cgacgttcgg gttcgacggg gcgacggcca cggcgccgaa cgtcaggagc ggcaggtcgt
     4441 ccgtcaggtt gacggggttc gcgtagtcgg cccccggccc gccctggcgc gtcaggagcc
     4501 agatcccgcc gttcgccgtc gcgacgtaga gcttcgaccc gtcgcgcgtg aaggcgtagt
     4561 cgcgcacccg tcccgcgtcc gtcgtcggcc cgagcgattt ccagccggcc gccgcgagcg
     4621 tcgcgttgag cggctgcggc cccccgtcgg gcgtccggaa gcggagacgt tcccggtaca
     4681 tctcctgcgc caccgaccgg aggatcggcg cggcggggat ctccgcacgt tcgagccgct
     4741 cccattcgcg tcgcgcctcc cagtagcgcc gtccctcgtg ggtcgcttcc ttctcgtgcg
     4801 ccctctcggt cggggcgccg aggaggacga ggttcgggag gttcctcttg ccggagaacg
     4861 ggatcggccc gtccaggacc gggggcgtcg tcggcccggc cgccatggcc gagtcggcgg
     4921 cgaagagcag ggcgctggcg acgaggagga aacgacggcg atgctgcatg gaaacctccc
     4981 gcatcggggc cgccccgggc ggcccccttc cgatcgaacg gcttcggatt tggcgggatg
     5041 ctaacggaga cgggtcgcgg aggggagcac gagctgcgtc gggcgatgac gcagatcacc
     5101 ctccccggac gtggccgtcc ttcgggcggc cgacccccgg cgacggttca ggcttcggga
     5161 acgccctccg gctcgctccg gtggagctcc tcccacgtgc cccgcctccg gatcacccgg
     5221 accgcgtcgc cgtcgacgag gacctcggcc gggagcggtc gggcgttgta ctcgctcgcc
     5281 atgacgaagc cgtaggcgcc ggcgtcgagg aaggcgacga ggtcgcccgc ggcgggccgc
     5341 gggaggaggc gcgcttcggc gaggatgtcg cccgtctcgc agacgtcgcc ggccacgtcg
     5401 aggagcatcg gctccccgtc gcgaccgacg atccggaccc ggtgcgccgc gccgtagagc
     5461 gccggacgca tcagcgtgtt gaagccggcg tcgacgttga cgaaggtctt cacgggagtg
     5521 gtcttcacgg agttgacgcg cgcgagcatc agcccggcct gcgcgacgag gtagcgcccc
     5581 ggctcgatcc agagctcggg ctcgtaaccc aggcgggaga cctcgtcgtc ccagatcggc
     5641 ttcagggcgc gcgcgaggtc ctccggagtc atgacctcct gcccgtccgc gaaggggatg
     5701 ccgaggccgc cgccgaggtc gacgaaggag agcgtcagcc cgagctcgtc cttcagctcg
     5761 cgcacgaacg cgagcatccg gcgggccgtc ttctcgtacc ccgcggtctc ggtgatctgc
     5821 gagccgatgt ggcagtggac tccggcgatc gcgagggccg ggagcgcccg ggccttcgcg
     5881 taggccgcgc gggcgtgccc gccgtcgagg tggacgccga acttcgactc ggggatgccg
     5941 gtgttgatct ggtgaagggt gtcggggtcg actccgggat tgatccggaa ggagatccgc
     6001 gcggtcgtcc cgaggcgaag cgcggcggcg gagacctgct cgagctcgtc gaggttgtcg
     6061 acgttgagga ggacgtcgtg cccgagggcg aagtcgatct ccgagggact cttggaggag
     6121 gaggtgaaga ggatgtcatg gcccgtggcg ccggcccgga gcgccgcccg gatctcacct
     6181 tccgacacga cctcgggcgc gagcccctcg gcgaggagct gccgcacgat ctcgacgttc
     6241 gtgttcgcct tcagagcgta ccgcagcgcg aggcgcggga atcggggcgc gaacgccgcg
     6301 tggagcgcgc gcgccctctc gcggatcgtc tcggcgtcgt agacgtagag cggcgtgccg
     6361 tgcccggcgg cgagccgggt cagcgccgac acggcctcgg ggtgggccgc ttcgcccgcg
     6421 cggatgcgga ggaggccgtc gcgcagcgtc atcgggggcc tcttcgagcg cccgtgcggt
     6481 cgagggcggg gagcggaggg acggccgggg gcgcgggcgc tgcgttcttt ctcatggggc
     6541 cgggcgattc tgcggcgccc cggccccgcg gtccagccga cgcggcctag aatcggactg
     6601 tgagaacacg ccgcagcctg ctctccgagg ccgccctcgc gtcggccttc ctggcgctgc
     6661 cccgatccgc ccgcgcggcg gcgccgccat ccggcccggt ccggccgaag tctctcgctc
     6721 cgggcgcgcg ggtcgcgctc gtctcgccgg cgagccccgg ggcggaggcg gagtccgccg
     6781 tcatcgccga ggaggtcgtc gcctcgctcg gcctcctccc gaagcggatg ccggcggccg
     6841 cccggcagac gatgtacctc gccgggacgg acgcggagcg ggccgccgac ctgaacgccg
     6901 cgttccgcga cccgtcgatc gacgccgtct ggtgcatccg cgggggttac ggctcggggc
     6961 ggctgctgcc gctcctggac tgggagacga ttcgcaggaa cccgaagccg ctgctcggat
     7021 tcagcgacat caccgcgctc ctgaacggct tctacgcgaa gacggggctc gtcacgttcc
     7081 acgcgccgac ctgctcggag aacctctccg actacacgct cgccgagctc aagcgcgtcg
     7141 tcttcacgcc ggaagcggcc ggtgtcgtcg ccgccgcccc gcccgccgag ccgaaggagg
     7201 gtttcgtcga ccgagaggac cggctgcgcc ggatcgcccc cgggagcggc cggggccgcc
     7261 tcgtgggcgg gaacatcagc gtcttctcga tgctcgtcgg gacaccctgg gagccgccgc
     7321 tcgccggctc gatcctcttc ctcgaggagg tgggggagga gccgtaccgg atcgatcgct
     7381 ggctgacgca cctcgtcctg acgggacgcc tcgcgaagtg cgccgggatc gtcttcggca
     7441 agttcaagga ctgcggtccg ggcgacgagg ggagctacca ggggacctgg acctggcagg
     7501 aggtcgtcgc cgaccgcctc gggaagctcg gcattccggt cctcgcgggc ctccgcttcg
     7561 ggcacgtcgc cgacaagtcg accctcccgg tcggcgtcct cgccgagctc gacgtcgccg
     7621 ccgggaccct gtcgctcctc gagcccgccg tctcgtgagg gaggggcgcc gggcacgccg
     7681 ggcggggcgg gccgcacggg cgctcctcct cgcggtctcc ctggcgcctt cggtcgcgag
     7741 cgcggggccg gaccgcaccc cggccgggct cccgcgcgtc ctcgacggcg gctggcgcgc
     7801 ggccgacgcc gatccccccg acggaatcgc ggggatcgac cggctcgact tccgtcccgc
     7861 cgacccggtc tcggaccagg ccccgcgcgg cgaggtccgc tggtatcgga tcctcgtcga
     7921 cctctcgccg ttcgtggggc agccgctcgc gttcgcggcg gacggcctcc gcgacgtcga
     7981 cgaggcgtgg ttcgacggcg tcccgatcgg cggcttcgga gcgttcccgc ccggccccga
     8041 gacggcgcac ttcgtctcgc gcgtctaccc gctgccgacc gaccgcgtcg acgaggccgg
     8101 gccgcgggag ctcgtcctca gggtctggca cgggaagagg gacggaagcg tcttccgttc
     8161 gcctcccgtg atcgaccggc tcgaccggct cgagaggagc cgatcgctcc gcgaccagag
     8221 cctcgtcctc ttcgtctccg cggccttcgt cgtcgccggc ctcctcttcc tcttcgcgtt
     8281 ccacgcgcga agccctgcgg actacctgct cttcgccggc ttcgccgtcg ccctcgggct
     8341 ctgggtgacg acggggcact cgcgctgggg tgagctcccg ttcccgctct cgaccgtctt
     8401 ccgggcggga ctcgtcgcgc tctcggccac cgcggtctgc tacggggcgg cgatgctccg
     8461 gttcctgggc gggatgcgcc ccgtccaccg ggtcctcctc ccggcgctcg gcctcctcgc
     8521 gcccctcgcg gtcttcctgc ccgacgccga ggacctcgtc ctgcccgtct tcgtcttccg
     8581 ctgggtcttc gcggcgctcc tcgtcgacct cctcgtccgg ttcggcatcg cggcgttcgg
     8641 acggcgacgg ctcgcgcgga cggatctcct cggccacgtc gccttcgttc tcgccgcggt
     8701 ccccgtcgcc ggtcttcttc ccggagctga gctgccccgg atcgacctgt cgggccgcgt
     8761 cctcatgttc ggcctcctct tcctcggcct cgcctcgacc gtcctgctgc gcatgtcgga
     8821 ggagatccgg cggtatcgcg tcgcggggct gacggatccc gggacgcgcc tctggaaccg
     8881 cgaggcgctc tacgacgaga tcgccgagcg gggagaggcg gttcgccggg gcaagggggc
     8941 gggcttcggt ctcctcctcg ccgacctcga ccggttccgc gagtggaacg acacgaaggg
     9001 gcgggtcgcg ggggaccggg tcctccttcg ggcggcccgg gccctgatcg acgcctccag
     9061 gccgaacgac ctcgtcgcac ggtacggcgg cgacgagttc gccgtggtcg tcggggacgt
     9121 ggatcgcgtg tcgctccccg cgctctccga gaggctgcgc agcgcgatcg gggaggcggt
     9181 gaaggacgag agcggcgggc tcctcacgag cgcctcgatc ggcacggagc tctacgaccc
     9241 ggagcgtcac cggacgcccg aggacctcct ccgcgaggcg gaccgggctc tctgggaggc
     9301 ccgggagaga tcgcgcggga cgacgccgac ggttcgccag acgccgtccg gcaagtggtc
     9361 cctgcgtctc tgatcggttc cgtagactcc gtcaccgtga cgaagcacgt ccggcgactc
     9421 gccctgccgc tctccgcgct cctcctctcg attccgatcc gggccgacgg cccgctcccc
     9481 gtcgacacgc gggcggccgt cggggccctc gccgcgaggg gagcctctcc gacccgagcg
     9541 gtcgagtggg tccggcggct gacggacgag gtgggcgccc ggccctccgg ctcgcccggc
     9601 gaccgggccg cgatcgcctg gggccaggcg ctcctccggg agctcgggtt cgtgcgcgtt
     9661 cgagccgaag aggtcccggt gagcgtctgg gagaggggcg tcgagacggc ggaggtcgtt
     9721 gcgccgttcc gccagccgct cgtcgtcacg gcgctcgggg gaagcgtcgg cacgccagcg
     9781 gaagggatcg cggcgtccgt cgtccgcatg gagtcgctcg aggcgctgga ggaggccgtg
     9841 aaggccgacc cggacgtcgt ccggggtcgg atcgtcttct tcgcgcggcg gacgatgaag
     9901 gcaggccagg gtccgggcta cggcgcgaac gtcccgatgc ggacgaccgg ggccgcgaag
     9961 gcgggtccgc acggcgccgt cgccgtcctc gtccgctccg tcgggacgtc acggagccgc
    10021 ttcgctcaca cgggctcgac gcgctacgag gctggcgggc cgcgcatccc ggcggcggcg
    10081 gtctcgaacg cggacgcgga cctcctcgag aggctcctcg cgcgcgggcc tgtgacggtg
    10141 cggcttgttc tcggctgccg gccgctcccc gacgggacct cggcgaacgt cctgggggag
    10201 gtgagggggc gggaacgccc cgacgaggtc gtcgtcctcg gcgcccacct cgactcctgg
    10261 gacctcggga ccggggccgt cgacgacggc gccggctgcg ccatcgcgat cgaggcggcg
    10321 cgcctgatcg cggaggctcc gaggcggccg cgccggacga tccgcgtcgt cctcttcgcg
    10381 aacgaggaga gcggcggggc gggcgggcgc gcgtacgcgg agagacacgc ggcggagctg
    10441 ccaaagcacg cggcggcttt cgaggccgac agcgggacgg gaacgccgct cggattctcg
    10501 tggaacgccg cgggaacgct cgagccggct ctcgccgccg tcgccgagct ccttcgtccg
    10561 ctcggcgccg aagggcttcg aaagggcggg tacggcggag tcgacgtggc gcccatggcc
    10621 ccggccggcg tccctctctt cgggcttcgg caggactctt ccacctattt cgacgtccac
    10681 cacacggccg acgacacgtt cgacaagatc gagcccgcct cgctcgaccg gtccgccgcg
    10741 gcgatggccg cgatggccta cgcgttggcc gacctgcccc ggatccacgc gcggccggcg
    10801 gagccggcga cggagggccg gtgaccctcc cgtcgctccc ctctctcccg gcggccggcg
    10861 gcgcccccgc cgccgccccg cccccggccg gtcccgaggt cgagctcgcc atcgccggca
    10921 tgcactgcgc ctcgtgcgtc gggcggatcg agaccgccct cgggagggtg ccgggagtct
    10981 cgctcgcggt cgtcgatctc ctctcggggc gggcgcgcgt tcgcctctcc gacccgtcgc
    11041 tccccgtcga gcggctcctc gaggcggtcg tctccgcagg ctacgaggcg aagccggcgg
    11101 cgagcggaga gtcgacgggg ctcgaggagc tcgtcgacgc cgagcaggcg gagcgggcgt
    11161 ctcagctcgc ggcgctgacg cggaaggccg cgttcgccgg cgttgtcgcc gtccccgtcg
    11221 cgctcctctc gatggccgag gtgacgttcc cggggcggga cgtcctcttc ctcgtcctca
    11281 cggcgctcgt cctcgccgtc cccgcgcgtg acatcctcgt ctccggagcc cgcgccgccg
    11341 cgcgcctcgc ccccgacatg aacacgctcg tcgccctcgg cacgaccgcg gcgttcctga
    11401 cctccgcggc ggcgacgctt ttcccgggcg cgctccacgc ggaaggggag cacgcgccgg
    11461 tctactacga ggcggccgtc gtcgtcgtcg ctctcgtcct cctcggccgc ttcctcgagg
    11521 agcgggcgaa ggggagggcg ggggaggcga tccgcgcgct cgcgcggctc ggggcgcgaa
    11581 aggcccgcct cgtccgtgac gggggcgacg tcgaggtgcc gctcgagcgg gtccgcgcgg
    11641 gcgacctcct gcgcgtcctg ccaggcgaga aggtgccgac ggacggcgtc ctcgtcgagg
    11701 gctcgacggc ggtcgacgag tcgctcgtga cgggagagtc gatccctctc gagaaggagc
    11761 cgggcgaccc gctcctcggc gccaccgtga acgggaacgg cgccgtcgtc atgaaggcga
    11821 cgcgcgtcgg gaaggagacg gcgctcgcgc aggtcgtgaa gctcgtgcgg caggcgcagg
    11881 cgacaaaggc gccggtccag aggctcgtcg accgcgtcgc cgcggtcttc gtcccggcgg
    11941 tcgtcgccgt cgcgctcctc tccttcgcgg ggtggatgct cttcggcccc gagccgcggc
    12001 tcctgcgtgc cctcctcgcg gtcacgtcgg tcctcctcgt cgcctgcccc tgcgccctcg
    12061 gcctcgcgac gccgacggcg ctggtcgtcg ggacggggcg gggcgcccgg atggggctcc
    12121 tcctgcgcga cgccggcgtc ctcgagaagg cggcgcgggt cacggcggtc gtcttcgaca
    12181 agacggggac gctcaccgcg gggcgaccgg cggtgagggc ggtccgcccg gtcgacggca
    12241 ctggcgaagc cgatctcctc ggcgccctcg cggccctcga gagcggctcg gagcacccgc
    12301 tcggaagggc gatcgtcgcg gaggcgaacg cgcgcggcct cgcgttcgag cgcgcgacgg
    12361 acgtgaaggc ggtgccggga ggcggcgtga ccgggacggt cggcggcgcg ccggcgctcg
    12421 ccgggtccga ggcgttcctc gcctcgcgcg gcgtctccgt gccggaggcg ctcctcgggg
    12481 aggcggacgg cgggacgctc gtcctcctcg ccgtcggcgg cgcgctccgc ggcgccgtga
    12541 cgctcgccga tccgctcaag gcgcacgcgc gcgaggcgct cgcggggctc tcggcacgcg
    12601 gcctgtcgct ccacctcctc tcgggcgacc ggccggccgc ggtggcggcc gcggcgcgcg
    12661 aggcgggtat cccggaggcg aacgtcgccg cacgcctctc cccgtcggag aaagccgagc
    12721 gggtgagagc gctgcgcgcc tcgggcgcgg tggtggcgat ggtcggcgac gggatcaacg
    12781 acgcccccgc tctcgccgcc gccgacctcg gcgtcgcgat cggctccggg accgacgtcg
    12841 cccgggccgc ctccgacgtt accctcgtcg gcggggcgct cgcggggatc gggacggcga
    12901 tcgacctctc gaacgcgacg ctccgcaacg tgcggcagaa cctcttcctc gcgttcgcct
    12961 acaacgtcgt cctgatcccg ctcgccgccg gggcgttcta ccccttcacc gggtggaccc
    13021 tgaacccgat gctcgcgggc ttcgcgatgg ccgcttcgtc cgtcagcgtc gtgacgaacg
    13081 cgctccggct ccggcgcttc gggaaaggct gaggcggggc ctacgggagc gccagctcgc
    13141 cgcggacgac ggtgacggcc gagccggcga gggtgacccg ctcgccgcgg cggccgaccc
    13201 ggacggtccc tccgcgagcc gaggcctggt agccgacgag gtcgtccttc ccgagcttct
    13261 ccccccagta cggcccgagc gcgcagtggg ccgagccggt gaccgggtct tccgggacgc
    13321 cgcagcccgg cgcgaagaag cgcgagacga agtcgtgcgg gctcccggcc tcggcgcggg
    13381 ccgtgacgat gacgccccgg taggggactt cctcgaggag ggccaggtcg gggcggagcg
    13441 tccgcacctc ggcctcttcg gcgacctcga agaagaggtc gaatcgtgtc ttccccgaga
    13501 agtcggggga gatgccgaga gcgcggatcg ccttctccag cggcgggaac ggctcggccg
    13561 gagtggccgg gaagtcgagg acgatcgtcg ccccctcgcg gacggccgtc aggaggccgc
    13621 tcttcgtccg gaaacgcgcc gtggcgtccg gcgcgaggag gccggcctcg aagaggacgt
    13681 gggccgaggc gagcgtcgcg tggccgcaga ggttctcctc gacctcgggc gtgaaccaac
    13741 gtagccgcca ctcgccctcc tcggggagga ggaaggccgt ctcggcctgg ttcatctcca
    13801 tcgccacgct ctgcaggacg gcgtcaggga gcggctcgcg aaggaggcag actccggcgg
    13861 ggttgccggc gaacgggcgg tcggtgaagg cgtcgacgag gtggagcggc agcgccatcg
    13921 ggcgacgata cgcccgatag gacggacggg cgatcccggg ggcggggagg cgccgcgaaa
    13981 cctccggccc tcccgctcgt ctgacccgtc gtgagaccct tgcgggcggt gagcaccgtt
    14041 cccgacgacg atgccgacct gatgggccgg gtgaaggccg gggacccggc cggggcggag
    14101 ggcctcttct cccgctacgc gggcccgatc ctcggcttca cggagcggat gctcgggagc
    14161 cgggccgagg ccgaggaggt cacgcaggac gtgttcctga agatgatcac ccgctgcgag
    14221 cagtacgacg gccgcgcgtc ggtctcgacc tggctcttcg cgatcgctgc caacgcctgc
    14281 cgggaccgtc tccggagcgc ctggaggacg acctcggtcc cgctcgaggc cgtgccggag
    14341 ccgaaagggg aagggtcgat cgagggaggg ctcctgcaga aggagcagcg ccgggtcgtt
    14401 cgggcggctc tcgatcgcct cacgcgagag cagcgggagg cgctcctgct ggcccgctat
    14461 caggggatgc cctacgccga ggtcgcccgg acgctcggca tcagcgaggg ggcggtcaag
    14521 acgcgcgtct tccgcgccgt ggagacgctg cgcgccctct tcgccgagga aggaggtccg
    14581 tcatggactg ccgcgaggtc ctagagaccg ccctcgcccg caccgcgggt gagccggcgg
    14641 acacccgtca ggccgagctg gagcatcacc tcgagcggtg cgagagctgt cggagggcag
    14701 ccgcggccct cgacgagacg tgggagagga tcggcctgcc ggagccggac gtctccatcg
    14761 cctttcgcga acggacgatc ggacggatgc aggaggcgct cgcccgccgg aacgtcgtgt
    14821 cgttccggcc gcggacctgg tggaggcccg tgcttcaggc cgccgcgctc ctcgtcacgg
    14881 gggtcggcgg tttccttctg gcgcgagcga gcgccccgcc ggcggacttc cccgcagccg
    14941 cggtcgaacg agcggggacg ccctccgtcg ccgtcgtctc cgagaggcag ctcgacgcgt
    15001 cgcgcgtcgc gctcgacctg tccggaaacc cccggctcct gaacgtcgcg ttccgtccgt
    15061 ccgcggggga cgggcgcctc tcgatctcct tcgacgtcac gacccgctac cacgtctccg
    15121 gccgggccga ggaccgggcc gtcgccgcgc tcctcgccta cgtcgtggac gccggcgcgg
    15181 cgaccgaggg cgcgcgcggc cgtgccatcg acctcgtctc ccgcgagctc ggcgagcgtg
    15241 ccgccgcctc gcccgagatc gtcgaggtcc tcgttcgcac gctgaaggac gacgggaacc
    15301 cgggcgtgcg gaagaaggcg gccgaagcgc tggtccagct gccgccgtcg cccgcgatcc
    15361 gtgacgccct ggccctggcg ctccggcggg acacgaatcc cgccgtgcgg atgatcgccg
    15421 tcgaggggct cgcgaaggcg gccacgacgc tgcgcgaccc gctcagcatc gagacgctgc
    15481 gcgagaaggc gggcgacgag acggagccgg gcttcgtccg cgtgaaggcg gcctcggcgc
    15541 tgcgggagat cccgctctga cgtggatcgg ggggacccgt accaccgggt ccccccgcgg
    15601 cgccgccggc gcctccccct ccgcgcggat cggagggggg cgaagtgcgt ggatcgggga
    15661 acccgttctt ccgggtcccc tcgcggcgcc gccggcgcgt gacctttgac ggactcgaac
    15721 gtcagaccgg catgaacacg gagaccacca tgacgacgat gagcacccgt tccgtctccc
    15781 gcacctggac ggtcttctgg atcctcctga tcggagcctt cctcgtcgtg tcgctcgcct
    15841 ggcccgcgcg ggccgaccgc ctcggggcga ccgcgagccg gacggagacg ttcacggcgg
    15901 ccgccggggt gaagtcgctg acgatcgacg gcgtcagcgg tgacgtcgag gtcgccgccg
    15961 gtgcgtcgtt cgcggcgacc gtcgaggtga cggtcaaggc ggacggcgac gcccgcgcgc
    16021 gggagatcct cggaaagacc aggattgcct tcgagaacag cggcggggag ctgacgctcg
    16081 ccacggtcga gccgggggcg gagtactccc gcgaggggcg gaggacgcgc cttcgcgtct
    16141 cccacgacgg cgaccggcgc tggcgcgtgg aagcccgcta ccgggtgacc ctccccgcgg
    16201 gggcgagtgc gacggtctcg ctggtgaacg gggggatcgt cacgaagggc ctcgacggcg
    16261 tccaggagct gacgacggtc aacgggcgcg tcgcggcgtc cgacgcccgc cggagcgcga
    16321 cgctcaagag cgtgaacggc accgtggaag gagcgttcac cgtccttccc cgcgacacga
    16381 aggtggtcgc ggagacggtg aacggcggcg tgaagctgac gcttccctcc gacgcggcgt
    16441 tcgacctgaa ggcctcggcg atcaacggcg gcatccgttc gactttcacc ctgccggtgc
    16501 ggggcggagc ggacgacgac gtcgcggtcc gcgagatcga ggcagcggcg catgccgagg
    16561 cggagcgggc ccgggccgcc gccgaccggg cgcgcgagag gtcgcggaag gcgcgggccg
    16621 ccggctcctc cgcggacgag agcgcggact cctgggaccg cgagtggaag gagttcggcc
    16681 gcgagatggc ggagttcggc cgcgagatgg ctcgcctgag ccgcgagatc tcccgctcgg
    16741 tctccggcag cttgaaccgc tcctacgaag ggacccaccg agggggcggg gccgccgtga
    16801 agtgctcgac gatcaacggc ggcgtcctcg tcctctcctc cgggagcgcg gagtcggcgg
    16861 cgaagtccct cgtgccgaag cgcggggagg gctgggccgg tgcgcgcgtc gccccggtgc
    16921 cgcccgcccc gccggtgccg ccggttcctc ccgtgcacgt gggccgcgtt catgggcgcg
    16981 aggacggctc ggtcgtgaag ggggacgtga gcggagacct ccacctctcc ctcccgaccg
    17041 gcgacgttcg agccggaagg gtctcgggcc gagcgaccgt gcggacgagc tcgggcgaca
    17101 ttcggatcgg ggccgtcggg aagggggccg acctctcgag ctcgggcggg gacgttcggg
    17161 tcgagcgggt ggacggaggg ctcacggcgt cgacgctcgg cggcgacatc gtcgtgggcg
    17221 acgcctcggg ggaggtccga ctccggacga tgggcggcga cgtgaggctc cgctccgcga
    17281 gcgggccggt caccgcgaag acggcggggg gcgacgtccg cgtcctgcgg gctctcggcg
    17341 ccgttcgggc cgagaccgcc ggaggggagg tctactgcga gatcgcgtcg aaggacgcgg
    17401 ggccggtgga gctctcgacg ggcggcggcg acgtgaccgt ggtcctcccg gcgaacttcc
    17461 gcggcgacgt cgaggtagtc gtccggagcg tcgacgcgga cggggagtac gtcctctccg
    17521 acttctccga gatcgggatc ctccggcagc ggggacaggg gcgcggggtg gtccgcgcgg
    17581 agggccgcct gaatggaggc ggcgcgaagg tcaccgtcag cgcctcctcg gggaccgtcc
    17641 acatccgaaa gggaccgccc gcctgacgcc ccgacgtgtg gcctcgagcg gtcgcgtccg
    17701 cgccccctcc tcggcggggg cgcacccgtt tgctaccctc cggccgggcg ccggcgcctc
    17761 gcgccggagc cggccccgcg aatccagctc ccggaggtac ccatgaagag attcttcccc
    17821 gcagccctcg ccgccctcgc gatgctcgtc gtcgcccccg cgtcggtcgc cgcgatcccg
    17881 gaaaagggct ccatctcgat tttcgcgggc gtcgggcccg cgatcccgtt cgaaggtgac
    17941 tacaaggtcg gcttccacct cgaggccgga ggcgagtact acctctcgaa cgtcctcgcc
    18001 ctccgcggaa ccctcggcct gacccgctcc aacgcggacg gaggctcggg cgtcacgctc
    18061 ggtggtctcg aagcgtccgc cgtctactac gcgcgtcgcg ggaagtggag tccgtatgtc
    18121 ctcgccgggg tcggcatcca cacggtcgac ccgccgggaa ggagcgcctc tcagcgcctg
    18181 agcgcccacg tcggcggcgg cacggagtac tacctcgacc gccgaacggc tctgaccggc
    18241 gagatcaccg gccgcttcat cggcagcgca ggcgaccgga cgtcgagctt cgcctccctc
    18301 gcggtcggga tcaagtacca tttctaatgc aagatattca cggagaacga cttcggtctc
    18361 tgcggcgagg ccagaccgaa gcgtacccgg acgagatcgg cgggtaccca tgaaatcgga
    18421 cttgacaggt ccgaattctc gcttagcttg tcaaggatga tgcacccccg tcgccgtccg
    18481 gcggctcgag ccgtggccct cgtagcggcc ctcctgactc tcctcggggt gcagctctat
    18541 cttccgacgg cggtcgctgc ctgtggtctc gcggagacgg actgctgctg tgcggccgaa
    18601 gccgccggct cggccgaaaa ggccccggcg gacgactgcg actgctcgat ctcgcggccg
    18661 gcacccgcgc cggctgcgga cgtcgccccg gccgcggcat tcgcgccgcc cgccctctcg
    18721 gccgagaccg tggacgaacg gcgaacggag gggccggcga ctggctgccg gctcccgact
    18781 cctccgtccc gggcccgctc ggcgccgact caggcgctcc tcgagacctt ccggatctga
    18841 gacctcgggc gttcgcgctc tcgtccgtcc cgttcgggac ggcccgatga ccgcatcccc
    18901 gaggtgtcga gatgaagctg gtcccgtggt ttccgcggag gttcgccccg gcgacgctcg
    18961 ccgtcgccgt cctctcactc tctccggacg tgcctgcccg cgcagccgtt gcggctccgt
    19021 cccccgctcc cgttcccgcc ccggtgcggg agaccgcttc gaccgtcgag gccctcgtcg
    19081 ccgaggcgct cgaacggagt ccggccctcg ccgccgttcg agcctccgtc gcggccgctc
    19141 gcgagcggat cgccccggcc gggacgctcc ccgacccgat gctccggctc tcgtacgaga
    19201 acgacggggc gtccctctcc ctcggcacgg aggcgatgac ccggctctcc gtcatggccg
    19261 agcagccgat cctgtggccg ggaaagctga gtcttgcggc acgaatcgcg gaggccgacg
    19321 cggacgttgc cgaggcggcg atcgcccgcc tcgggctcgg cgtggaggcc cagacccggc
    19381 gggcggcggc acgcctcctc gaggcgcggg cggcgggcga gctgacccgg gagctctcgg
    19441 agaactggga tcggatcgag tcggtcgcgc ggagccgcta cgcggcgggc ctcggggcgc
    19501 agcaggacgt cttccgcgct caggccgagc ggacgcgcct gcttcagcag ctccaccgcg
    19561 acgagggggc cgtgcacgcg gccgaggcgg agctccggcg tctcctcctt cgcgagccgg
    19621 ggacgccggt cgagctcgag gagcggctcc acgagacgag gcccgagccg cccgacgcgg
    19681 acgtcgagct ggcgcgggcg gaagccgaga gtcccgagat cgaggtggcg cgaagggcgg
    19741 ccgaacgcga ggctctcgcg atcgacctcg cgcgacgcga ccggatgccc gacctcgtcg
    19801 cctcggtcgg ctacatgaac cgcggctctc tcccgatgat gtgggccgtc ggcctcgggg
    19861 tgacggtgcc gctcttctcc gggaggaagc aggagccgcg tctccgggag gcccaggcgc
    19921 ggctcgcggc cgtgcgggcg gccgaggccg acgtccgtct ccggctccgc gcgcggacgg
    19981 aggcgcaact cgccctcctc gcccagctcg ccgaagaggc ccggctcgac cgggaggcgc
    20041 tcctcgtcca ggaccggatg gcggtcgagg cggctctcgc gagctacgcc tccggctccg
    20101 tccccttcgt caccgtcctc gaggcgatct cgaccgagtt cgccgaccgg cgcgcggccc
    20161 tcgggcggct cgccgcgttc ctcgtcgcgg aagcggacct ttacgagcgc tccctcgacg
    20221 gcgctccgat ggccgccgcc tccgctcttc cttccgcccc cgcgccgccg gccgcgatga
    20281 acgccgccgg cgggatgtga ggagaccccc gatgaagcac tcccgaatcg ttctcgtcgc
    20341 gctcgtcgcc ctggccgttc cgaccgtctt cctcgcggcg ggctgcggag gaacgaagtc
    20401 gcagatcgcg gtcgcgaaga cctggatctg ccccatgcac ccggacgtcg tgaaggacgg
    20461 gaaggggagc tgcccgatct gcggcatgga cctcgttccg aaggacgatg cgacgcccgt
    20521 gccgcaggcg gcttcccacg aaacgtggat ctgcccgatg cacccagaga tcgtgaagga
    20581 ccgcaaggag agctgcccga tctgcggcat ggacctcgtg aagcgggagg aggacgcgtc
    20641 ggcgggcggc gcgggagaag cgggcgttcc cggcttcgcg accgtgacga tcgacgcccg
    20701 caagcggcag ctcctctcgc tgaagacggc gagagtcgat cgggggacct tcggcaccgg
    20761 gatccggacg gtcggccggg tcgctttcga cgaacggcgc gtccaccacg tccatacccg
    20821 ctacgaggcc tacgtcgagc acgtcgccgc cgacttcacg gggaagtacg tgaggagggg
    20881 agaggtcctc gcccacgtct acagcccgga gctcttcgcg actcagcagg agctcctcct
    20941 cgctcttcgt gccgtccgcg ccctcgggcc ttcggccgac gccacggcgc gggagggggc
    21001 cgatcgtctc ctcgatgccg cccggcagcg cctccgcctc tgggaggtga cggagaagga
    21061 cctcgccgcg ctcgagacgc gcggcgagcc gctccggagc gtcccgatct acgcgcccac
    21121 ctccggctac gtgacggcgc ggacggcctt ccacggcatg aaggtcatgc cggctgacac
    21181 gctcttcgac atcgtcgacc tctcgtccgt ctgggtcctc gccgacgtct acgagtcgga
    21241 gctgccccgc gtgagcgtgg ggcagacggg ccggatgacg ctcgcctacc gcccggggcg
    21301 gagctggacg ggcagggtga cctacatcta cccgtcgctg gacgagaaga cccggacggc
    21361 gaaggtgcgg ctcgagttcg cgaacccccg cgaggagctg aagccggaga tgttcgcgga
    21421 cgtcgtcctc gagagcgcgc cgcggacggc gctgaaggtg ccggacgacg ccgtcctcga
    21481 cagcgggacg cggaaggtcg tcttcgtcgc gaagggggag ggagtcctcg agccccggga
    21541 ggtgaaggtc ggcgagaagg ggggcggggc gtgggaggtc ctctccgggc tcgaggaagg
    21601 ggaggagatc gccctcgggg cgagcttcct cgtcgactcc gagtcgcgcc tcgccgcggc
    21661 cctcgccggg atgagggacg gcgcgacgcc ggccgcgggg cacgagggcc acgctcccgc
    21721 gccgtcgccc tccccgtcgt cacccggcac gggccacgac cactgagggg ccggccatga
    21781 tccggaagat catccgcttc tcggcggaga acaagtacct cgtcctcgcg ctctgggcgg
    21841 tcgcggtcct cctcgcgttc cgctccctcc gcaacgtccc gctcgacgcg atccccgacc
    21901 tctccgacac gcaggtcatc gtcctctcga agtgggaccg gagccccgac atcctcgagg
    21961 accaggtcac ctacccgatc gtcaccgccc tcctcggcgc gccgaaggtg aagacgatcc
    22021 ggggcttctc cgacttcggc tactcgttcg tctacgtcat cttcgaggac gggaccgacc
    22081 tctactgggc ccgctcgcgc gtcctcgagt acctctcgaa gatccagggg agtcttccgc
    22141 agggcgtgaa gacggagatc ggccccgacg cgacgggcgt cgggtgggtc taccagtacg
    22201 ccctcgtcga cgagacgggg cagacctccc tcgacgagct ccgctcctac caggactggt
    22261 tcctgcgcta ccagctccag tcggtgccgg gggtcgcgga ggtcgcgacg gtcggcggcc
    22321 tggcgcggca gtaccaggtc accgtcgacc cgaaccggct cgcggcgtac gggctctcgc
    22381 tggagaccgt gtcggacgcg ctccggagga gcaacgacga ggtcggcggc cggctcatcg
    22441 agatctccgg ccgcgagtac atggtgcggg ggcgcggcta cctgaagggg acggccgaca
    22501 tcgagaaggt cgtcgtgaag accgacgagc gcgggacgcc ggtgacgatc aaggacctcg
    22561 gcagcgtcgc gctcgggccc gacctgcgtc gcgggctcgc cgagctggac gggaaggggg
    22621 agaccgtcgg aggcatcgtc gtcatgcgct cgggcgagaa cgccctccac gtcatcgagc
    22681 gggtgaagga gcggatcgac gagctccggc cgtccctccc gcccggcgtg aagctcgtga
    22741 cgacctacga ccgctcgacg ctcatcgagt cggcgatcga caacctgaag gggaagctgg
    22801 tcgaggagat cgtcatcgtc tcgatcgtca tcctcatctt cctctggcac gtcccgagcg
    22861 cgctcgtccc gatcctgacg atcccggtct cggtcctcct cgcgttcatc ccgctccacc
    22921 tgatggggat caccgtcaac atcatgtcgc tcggcggcat cgcgatctcg atcggcgtcc
    22981 tcgtcgacgg cgcgatcgtc gaggtggaga acgcctacaa gaagctccag atctggaacg
    23041 actcggggcg gaagggggac ttccacgccg tccgcctcga ggcgctgatg gaggtcggcc
    23101 cgtcggtctt cttctccctc ctcgtcatcg ccgtctcgtt cctcccgatc ttcacgctcg
    23161 tcgaccagga ggggcgcctc ttcacgcctc tcgcctggtc gaagaacttc gcgatggcgc
    23221 tcgcggccgt cctcgcgatc acgctcgacc cggcgatgcg gatgcttttc gcgcggatgg
    23281 acccgttccg gttcggcttc gcgcacgacg ggcacggcgc gctcgcgcgg ctcgtccgcc
    23341 ttcccgcccg cctcgtcgag aaggccgtca acgccgtcgc cgtcgggacg taccacgccg
    23401 aggagaggca tcccgtctcg aaggtcctct tccggctcta cgaccccgtc tgccgggccg
    23461 tcctccgcca cccgaagaag gtcgtcgcag cctcgctcct cgtcctcgtc tcgaccgtgc
    23521 cggtctacct ctccctcggg tcggagttca tgccgccgct ccacgagggg acgatcctct
    23581 acatgccgac gacgctcccc ggcatctcgg tcaccgaggc cgcgaagctc ctccagacgc
    23641 aggaccgggt cctgtcgacg ttccccgagg tggaacgggt cttcggcaag gcggggcgcg
    23701 cggagacgtc gaccgacccg gcgcccctct cgatgatgga gacgacggtc atcctgaagc
    23761 ccgagaacga gtggcggccg aaggagcgct ggttctctcc gtgggcgccg gagtggctct
    23821 cggcggtcct ccggcacgtc tggcgcgacc ggatcacgta cgaggatctc atcggcgaga
    23881 tggacgccgc gctgaagatc cccggctcga cgaacgcctg gacgatgccg atcaaggggc
    23941 ggatcgacat gctcacgacc ggcatccgga cgccgatcgg cgtgaaggtc ttcggctcgg
    24001 acctcgccga gatcgagcgg atcggggccg aggtcgaggc ggcggtgagg acgctcccgg
    24061 cgacgcgctc ggccttcgcc gagagggtcg cgggaggcta cttcgtcgac ttcgacctga
    24121 agcgtgacgc gctcgcccgc tacggcctca ccgccgccga cgcgaacatg gcggtcatga
    24181 cggcgatcgg cggcgagacc gtgacgacga ccgtcgaagg gcgggcgcgc tacagcgtca
    24241 acgtccgcta cccgcgcgag ctgcgcgagg acctctcgag cctctcgcgc gtcctcgtcc
    24301 cgacgatgtc gggcgcccag gtgccgctcg ggcagctcgc cgacatccgc ctcgtgacgg
    24361 ggccgtcgat gatccgcaac gagaacgggc tcctctcggg ctacgtctac gtcgacttcg
    24421 acacgtcgaa ggaggacgtc ggccgattcg tcgagaaggc gaaggctgcc gtcgcggggc
    24481 gggtgagcgt ccccaccggg tactccctcg tctggagcgg ccagtgggag aacatggtgc
    24541 gggtgcggga gcggctccag gtcgtcatcc cggtcaccct cgtcctgatc ttcctcctca
    24601 tgtacgcgaa cacgaagtcg gtgacgaaga cgctcatcat cgtcctggcc gtgccgttct
    24661 ccgtcgtcgg cgccgtctgg tacctctggc tcctcgacta caacatgtcg atcgcggtct
    24721 gggtcgggat gatcgccctc atgggtctcg acgccgagac ggggatcttc atgctcctct
    24781 tcctcgacct cgcgcacgac gaggcgaaga agaaggggct cctgcgggac ctcccggacc
    24841 tcgtcgaggc gatcgtccac ggcgccgtga agcggatccg tccgaaggcg atgaccgtcc
    24901 tctcggcgtt cttcggcctc ctgccgatca tgtggtcgac gggggtcggg gccgacatga
    24961 tgaagaggat cgccgcgccg atggtgggcg gcctcgtcac gtcgttcgcc ctcgagctcc
    25021 tcgtctaccc ggccgtctac ctgctctgga agaagcggga gctgcctggg ccgggggcgc
    25081 ccggcgggga ggccgccgcc gcggcgtgag gaggcctgcg acgtgagctt ccagatggcg
    25141 tgtgatactc gccacagagg gaagtgtgcc cggtctggag aaggtgacgg cttcgcccgc
    25201 ggcttcggcc gggggccacg gcgacgaagg cccggccagc ctcgagcggc gcctccggtc
    25261 ctggaggccc ttcccgcacg aggtcgcggc ggccgtctac atcgccgcgg gcatcttcat
    25321 cctcgagcgc ctccccgtga cctacccgcg cgaggcgctg ctcctggccg cctcgtttcc
    25381 gattgcctgg atcgcagggt tcacggccgt cctcctggcg ggccgtgagg tcgcacggag
    25441 gctccggggc cggtcgcggc cggttgcatt cgccacggag gcgctcatcc tcgtccgggc
    25501 cgcactcgtc ctcgctccgg tcctctccgt ccacttcctg ctcaagagct tcatctggct
    25561 cgtgaacccg aggacgtggg acgcgcagct ctgggagctg gaccggatcg tccacttcgg
    25621 actcagcccg actctcttcc tgacggtcgc gttcgcggat ggggggttcc tccgcttcct
    25681 cgacgtcgtc tattcgggcg cttacttctt cctgatcgtc gtctcggtcc ccgttctcct
    25741 cgtcgtcccg cagccgagtc ggcggatggc gttcctggcg gcgtacacgt tcatctggat
    25801 ggcgggcagc gtcctctacc tcgcgttgcc gtcgtggggg cccgtcttcg tcgtctcgga
    25861 tctcttctct ggcgtgcttc ggcacatgcc cgcgaccgtg tcggtgcagg aggttctctt
    25921 cggcgagatc tcctccctcg tgaagcagcc tctcgcgccg cggatcgtcc gcttcggatc
    25981 cgtcgcggcc ttcccgagcc tgcacctcgc cgtcgtcacg gtcttcacgg tggcgagccg
    26041 atgggtctcg ggacgctggt tcctcgcgaa cctcgtcctc gtcgtcctca tgctcgtcgg
    26101 ctcggtcgtc accggctacc actacctcgt cgacagctgg gccggaatcg ccctcggact
    26161 cgcggcctgc tcggccggaa gccgcctctt cccgagccct tgcgccgcga cctgcggacc
    26221 cgactctcct cagcggccgt aggacgtctc tcggtcgagg atcgacaggg tccgcgcgta
    26281 gaggtgctcg acctcctcac ggctgttgcc gatcgcggtg acgccgatct tcccgtactg
    26341 ggagagggcg ccgatgaggt ggaagaggac gcccgactcc gtgttgtggg aatagtggag
    26401 gccgttgatc gtcaggatct cgatcaggtc ctcgggaagc aggccccggt aggcctccga
    26461 cttcaggttg tcggtcgcgc ggtagaactt cggaagccct cgcggcgaga agaagagccc
    26521 ggagccggga tcgagccggc cgcccgacag gaactggaga gcgaggtagg ggtgggtcgt
    26581 cccgcccatc cggaggttga tctcgagggc gtggagcgac cagtgggatg cgtcgtccgt
    26641 ccgcgtcgcg aggaagtcga cgccgaaccg gctgacgacg ccccggtccg cgaggacctc
    26701 gccgatgcgg tgcgcggccg cctgcgcctc gaggcgatag ccgtcggccg ccgggaagag
    26761 gcacccgagg aagacctgtc cgccgacgcc gccgagcacc tggtcgtggg tcgagatcgg
    26821 gacgacggcg ccgtgagggt cgacccggag ctggacgctc ggcgaggtct tctgatcggc
    26881 ctccaggaac tcctcgacga tgccccccat ccgcgcgaaa cgggagaagt agtcctcgtt
    26941 cgtctcccac gggacggcgt actcgaaccg ctgcatcgcc tcgcgcacgg tctcccggcc
    27001 caccgtcgtc gggtagcgga acagggcgtt cccttctccc gagaagctgt cgtccagctt
    27061 cacgaccgcc ttccgcgtcc ggggtcgtcg gcgatggagc tcgaggagcg cctcctcgac
    27121 gtcctcgagc gaccggaggt cctcgaagcc ttccggcaga gggacgccgg cctcgcggaa
    27181 gaccttccgg cttccggact tcgtcccgag gtggcgaagg gagggatcga ctccgttgag
    27241 cggcacgccg agaagaacgg cgagcttccg ttcgagcggc gtcgagttga agacggtgag
    27301 gtaggagcgc gccgggtcgg cgatgccggc ccggatcctc tcgatgaggc gagggcgctc
    27361 gaggatcttc tccgtcagcg atcgcgggga gtcgtcatag gcgcagagga gggtcagccg
    27421 ggcccgggcg tggctggccg ggatcccgac gaggagctgg aagtagtact cgaggacgag
    27481 cgggtggacc ggctgggacg tcacgaagac gacccgcgcg cgcgggttcc gcaggcggat
    27541 cagaaggaac aggaggcgtt cctcgtagta gggcgagccc tccagcttga ggagctcctc
    27601 ctggtccagc gtcagggagg ggacgacgat cgacgtatgg ggctcttcgt ccctcatcgt
    27661 gaaggaatcc cagacctcgc ggagcctcgg cttgaggcga tcgaactcct cgagctcgcg
    27721 ctcgagcgtc agctcgctca cgaagggcgg tttcgggatc acgcctgcgc attctagagg
    27781 cgtcggacgg agagccggca cggatcttga tccgagggcg cgaaagggag tacggaacga
    27841 tgagcaaccg gcggagggcg ggcttcacac tgatcgaggt tctcgtcgtg gtcgcgatcg
    27901 tcgggatcgt cgtcgcgatc ggggtcgtga attacctgaa cgcgatcgag cgggcacggc
    27961 agcgccgatc gatggcggac atgcggggga tctccaccgc catcgaggcc tatgcttccg
    28021 acctcaaccg ctaccctccg gcgagcgcct tcgttctccc cgccgggctc gatcttccaa
    28081 cggagaacct cgttgccgtc cggggttacc ttcagccgac ctacattcgg gcgttgcccc
    28141 tcgtggacgg atggaactcc tggttcacgt acgggacgag ttcgtcgttt tcggattacg
    28201 tcctacggtc gagcgggagg ttgggggtcg cccagacgga tccaccgttt gcggtgacga
    28261 cggactttcg ggacgacatc atcctcgtga acgggcggtt cgtgcagtgg ccggaagggg
    28321 ttcagcattg atcctcggcg acggatgggc gacacttttc gtgggcgagg cgggggtgcc
    28381 gcgcgacact ttttgtcggc accattcccg cgattgccaa ggcaggaccg ggataggcca
    28441 ccgcagcggc gtcaggacac tggcgcggga tttgcgggag atcacatagg aggctttctc
    28501 acatgaagcg gaacacgaaa ggcttcaccc tcatcgagct gctgatcgtc gtcgcgatca
    28561 tcggcatcat cgtcgcgatc gcgattccga acctgctgaa cgcgatccag cgtgccaagc
    28621 agcgccggtc gatgggcgac atgcggacga cggcgaccgc catcgaggcg tatgccgtcg
    28681 acctcaaccg gtacccgccg gcggccgcct acacgcttcc cgacacagtc atccccgacg
    28741 ccacgctcgc cggaatctcg gaatacctct ccccgaccta catcaagcag gtcccgcttg
    28801 ccgacggctg gaactcctgg ttcctggcca acgtcgacag ctcgggcagt ctctactccc
    28861 tcggttcggc aggtcgggat ggcaagtaca cggccaaaac ggccaccctc cttggcccga
    28921 cgaccgactt caacgccgac atcgtctacg tgaacggctc cttcattcag tggcctgagg
    28981 gcgtccagcg ctaaagcgct cggtcataaa ggtttgaaaa gcccccgtgg tttcggcccc
    29041 gggggctttt tcgtttcgtc gcaggggccg gggcggtcat ccccaaccgg tcatgcctcg
    29101 acccggggca agagctcgat ccgggcgatc cagcccccgt cgcccgtctc gacggccgcg
    29161 cccgagacct ggagcagccg gacgtcgacc ttcgggaggt ggaccgggag gttggcggtg
    29221 acgcgcctgt cgtcgaacgc gagccagcga ggcggccgaa gagccccgtc cactgcgcgc
    29281 acagcccgga ggacgtcgtg gacgagctcg acaaggaggg actcgtcgtc tcccgccgat
    29341 gcctggacgg gaacgacctg cccctggttt tctccttctg gaagcggtgc gccgtagacg
    29401 gcctcgaggg cgccggccag ggcgtctctg aagagaccct cgcggtcggg cgaggtcacc
    29461 aggagctcca tctcgtccgg actcgtcttc agctcgtgcg gcatggcgca actccttccg
    29521 cggccgagga tcttagcgct gacgctcgcc cgcggagacg gtcagaatcg ggggatgagc
    29581 gacgcacgcg ccccctccct gaccgaccgc ctccgcgact ggatcgtccg agaggggccg
    29641 ctccgattcg accggttcca gaacgcggca ctctacgacc acgaggctgg ctactacgaa
    29701 cgaccagggc gcgtgggccg gggtggcgac ttcgtcacgg gggcctcgtg gcacccggcc
    29761 ttcgcgcgct gcctcgtgcg ggcggccacc ggcgtcggcc aggagctcgg ggaggcggtc
    29821 gatctcgtcg acgtcggatc cggggaaggg gagctcctag cgttcctgga ggaggcgtcg
    29881 gcaggcaacg gatggcgcct tcgcggagtg gaggcctcgg ctgcacggcg aacggcggcg
    29941 catcgtcggg cgcccggcgc cacgttccac tccaccgtcg acgagctgcc gtgggggctc
    30001 agggggctcg tcgtcgctta cgagctgatc gacgccctgc ccgtccgggc ctttcggttg
    30061 ggcgcggacg gcgcgctccg ggagagatgt gtcggactgg acggggaggg ccggttcgcg
    30121 tgggcggagc agccctgccc ggacggcccc tccatcctcg ccgccctcgc gagacgggga
    30181 gcggcgcccg agccggacca gcttctcgag gtccggccgg ggacggcggc gctcgttcgg
    30241 gcgatcgcct cccggctcga ccgagggctt ctcctcttct tcgactacgg ggccccgaca
    30301 cgcgcgctct acggcccggc gcgcgtgaac ggaaccctcg aagcgttcct ggcccacggc
    30361 gtctcccgcg acgtcctgac cgatccgggg agccgcgaca tcacggcgtg ggtcgacttc
    30421 ggcgaggtgg aggaagtgct ccgcaccgag ggtctcgtcg tccacgggct cgtctcgcag
    30481 tcccgtttcc tcctcgcgac cggcatcgcg gaggagctcg cgctcgttcc gggcgaaggc
    30541 gtcgatgccc accgggtcgc cgagaggaac gccgtggcca agctcgtcag ccccggcgga
    30601 atgggggagt cgatccgcgt cctcgtcgcc gaacgcgaaa cgggactcgg gaagtccttg
    30661 atttccggcc ccttggtttg acttcgagac atcccgaccg gacaatccgc cgcatgtgac
    30721 gacgcgcaca acccaggggc ggaagcgccc ccggggaggc gcgtgcgcgg ggggggccga
    30781 ggccgtgcag agctatatcc cgatcctctt cttcgccgcg atcgcggcgc tctttccgct
    30841 ggccatcctc gtcgtgatgg cgcttctccg ggcgaaggcg ccgaacccga cgaagctgtc
    30901 gccctacgag tgcggcatcg cgtcggcgac cgacgcccac gaccaccggt tctcggtccg
    30961 ttactacctc atcgccgtcc tcttcgtcgt cttcgacgtc gagacggtct tcctcttccc
    31021 gtgggcggtc atgtacgaca agctcctcct gttcgggctg atcgagatgg cggtcttcct
    31081 ctacatcctg gtgctgggat acgtctacgt ctggaagcgg ggggcgctct cgtgggcctg
    31141 atcaaggacc tgccggacaa cgtcatcacg acctcggtcg acgccgtgat cggctggggg
    31201 cgcaagaact ccctctggcc gctgcagttc ggcctcgcct gctgcgccat cgagatgatg
    31261 gcggtgaccg acagccgcca cgacatggcg cgcttcggcg ccgaggtctt ccgcggctcg
    31321 ccgcgccagg ccgacctgat gctggtcgcc gggacggtga ccgagaagat ggccccgatc
    31381 gtccgccgcc tctacgacca gatggcggac ccgaagtggg tcatcgccat gggctcctgc
    31441 gcgacctgcg gcggcccgta ccggacctac gcggtgacgc agggggtcga ccggctgatc
    31501 cccgtcgacg tctacgtccc cggatgtccc ccgcggccgg aggccctcct gtacggcctc
    31561 atggagctcc agaagaagat ccagaagtcg cgcaccctcc ggcgcgcttc gtgaacgggc
    31621 gagcgcgatg agcgaagaca agacgatctc ccccgagact cccgcgccgg agccggtctc
    31681 gaaggcgccc gagaccccgg ccccggcggc gcccgagccg aggcccgtcc ctgcggcggc
    31741 gccggccccg gcggctgcag cagcggccgc gccgaagccg gcagtccccg cagtccccgc
    31801 cgccccgccg aagccggccg gcccgaagcc gaccgacgcg agcggcaagc ccctcgtcgc
    31861 cgctctggcc gccgcggtcc cgggctcggt cctcgatgcg tacgagttcg tcggggagac
    31921 gaccgtgaag gtcgatccgg gcaagctcgt cgaggcctgc cggcacttga aggacgcgga
    31981 ggggttcacc tacctcgtcg acctgacggc cgtcgactgg aaggagcgcg atccgcgctt
    32041 cgacgtcctc ctcctccttc actccttctc gcgtggtcag gagcgcctcc gcctgaaggc
    32101 ggcggtcggc gagagctgcc cgtcggtgac gggggtctgg ctgacggcga actggatgga
    32161 gcgcgaggtc ttcgacatgt acggggtccg cttcgacggt cacccggacc tccgccggat
    32221 cctgacctgg gacggcttcc aggggcaccc gctccggaag gacttccccg tcgaggggat
    32281 cgacacgggc gcggcgatct acccggaacg ctaccccgaa gggggggggc cggggcccaa
    32341 ggacccgaac cggaaggtgc tttcatgagc gagacgacgc ccggcaccgc cgcgcagttt
    32401 cccccgctgg aggggccgcc gctcctcggg ctccacgaga tggagctcaa cttcgggcct
    32461 cagcatccct cgacccacgg cgtcctccgc ctcgtcctga aggtcgacgg cgagcggatc
    32521 gtctcggccc gtcccgacat cgggtacctc caccgcggga cggagaagct cttcgagacc
    32581 gagacgttcc cgatggtcgt cccccacacg gaccggatgg actacgtcgc ctcggcgacg
    32641 aacaaccacg cctacgtcct cgtcgtcgag aagctcctgg gcgtcgaggt gccgaagagg
    32701 gcccagtaca tccgcgtcct cctcgacgag atgcagcgga tctcctcgca cgtcctctgg
    32761 ctcgcgaccg cggcgctcga cctcggggcg atcacgccgt tcttctacac gttccgcgag
    32821 cgggaggtga tcctcgacct cttcgaggag tactgcggcg cgcgcctcac gctgaactgc
    32881 atgcggatcg gcggcctccc ggtcgacctg acgccgggtt ggctcgagaa ggtccgcgcg
    32941 ttcaccgagc cgttcgagaa gaacgtcgac gagtacgagg agctcctgac cgagaaccgc
    33001 atctggaagc agcggacgat cgggatcggc gtcctcgacg agaagcgctg catcgagtgg
    33061 gggatgaccg gccccgtcct ccgggccgcc gggaaggcct gggacatccg ccgggcgatc
    33121 ccctacgagg tctacccgga gctcgagttc gacatccccg tcgggaagaa cgcggacacc
    33181 tacgaccgct acctcgtccg cgtcgccgag ctccgccaga gccggcggat cgtcctccag
    33241 tgcctcgact ggctcgagaa gaacccgggg acggagatcc gcgggaaggt gccgcgcgtc
    33301 atcaagccgc cggcgggcga ggcctacgcc gccgtcgagg cgccgaaggg ggagctcggc
    33361 ttccacctcg tcgcgagcgg cgggaacaag ccgtaccgcc tccacgtgag acccccgagc
    33421 ttcatcaacc tccaggccct ccccgagatg gcgcagggcc acctcgtggg cgacctcgtg
    33481 gccgtcatcg ggacgatcga catcgtcctc ggcgaagtcg accggtagga acgcgatgtc
    33541 cgagagcctc gtccactacg tcctcgtccc cttcgtgaag gggctcctga ccgtcatcct
    33601 ggtcgcgacc gcggccggga tcctgacctg gatcgagcgc cggggcctcg gccggctcca
    33661 gatccgcaag gggccgaacc gcgtcgggcc ggcgggcttc ttccagtgga tcgccgacgt
    33721 cgtgaagctc gtcttcaagg aagacgtcgt cccgtcccgg gccgagaagg tgatccacct
    33781 cctcgggccg ctcctcctct tcacgcccgc ggtcgtcgtc ctcggcctcg tcccctgggg
    33841 cccggagctg acgctcttcg gcgtgacgac gccgctccac gccgcggcgg acctgaacgt
    33901 cgcgctcctc ctgatcctcg cggtctcctc gatcggcgtc tacgggatca tcctcgcggg
    33961 ctggtcgtcg aactcgaagt acccgctcct cggcggcctc cgctccgcgg cgcagctgat
    34021 cagctacgag gtcccgatgg gcttcgcgat cgtcgccgcg gtcctgatgg ccgggtcgct
    34081 ctcgatggtc gagatcatcg aggcgcagaa ggaagcgggg atctggttcg tcttcccggg
    34141 gctcgtcgcg ttcttcctct acttcgtctc cggtgtcgcc gagacgaacc ggaccccgtt
    34201 cgacctcccc gaggccgaga gcgagctcgt cggtggcttc cacaccgagt actcgggctt
    34261 ccgctgggcg atcttcttca tgtccgagta cgggaacatg atctcgatcg gcgcgatcgc
    34321 cacgacgctc ttcttcggcg gctggctccg cccgttcccg aacgtcgcct ggctttcgtt
    34381 cctcgacgtg atcccgccct tcttctggtt cgcggggaag gtcttcctct tcctcctcgt
    34441 ctacatctgg ttccgggcca cgttcccgcg gtaccggttc gaccagctca tggcgctcgg
    34501 ctggaaggcg ctcctgccga tctcgctcgc gaacctcttc ctcgtcgccc tcgcggccct
    34561 ctggggcgcc gccgggctga aggtcctcgg ggcggtcctg gtcctcctcg tcgccggggg
    34621 gctcgtcgcg gcttggaggc gccccgcccg ggcgtccgtc acgacgcgga tggccgcgga
    34681 gaaggtggag gtgcgcgcgt gagcttcctc gaccgcgtcc tcctcctcga catcttcgcc
    34741 ggcctgcgcg tcacggcccg ccacgcgggc cgcaaggccg acacgatcga gtacccggag
    34801 cagagcaagg agccgtcgga gcgcttccgc gggatgttcc ggctcgacga ggagcgctgc
    34861 atcaagtgca cgctctgcgc gatcgagtgc ccgatcaaca tcatctacat cgactggcac
    34921 acggagaaga cgcccgaggg gaaggccgag aagaagctcg accgcttcga cctcgacatg
    34981 aagcggtgca tgttctgcgg cctctgcgag gaggcctgcc cgacgaaccc gcgctcgatc
    35041 tggctcgcca cgaagacgta cgagctcggc gtctacgagc gcgacgaggc gctctacctc
    35101 gacaagtcga agctcacgaa gtgggacggg aacgtgaagc cgtaccccgg cgtcgacccc
    35161 gacaaggcgc cggtgaaccc ggccaaggag aagtgacgtg gaaccgggcc tcttcttctt
    35221 ctggctcttc tccgcggtcg tcgtcgtcgg cgccgtcggc gtcgtggtca tgcgccagcc
    35281 gatccactcg gcgctcttcc tgctcctgtc gttcctcggc gtcgcctgcc tctacgtcca
    35341 ggctcgggcc gagctcgtcg ccgccgtcca ggtcctcgtc tacgcgggcg gcgtcatggt
    35401 cctcttcctg tacacgatca tgctcgtgaa cgtgaagcgg cagcggacgg agcgctacct
    35461 ccaccggcag gccgtcccgg cgctcgggtt cggcttcctc ttcctcgtcg tcgtcggcgg
    35521 ggtcgtcgtg aacgaggcgc gggcgctcgc ggggaaggcc ccggcggccg acccggccgc
    35581 gctcgccacg acggtcctcg ggagcgagac ggtcctcggg aacacgcagg cggtcggctg
    35641 ggtcctctac cgcgactacc tcctcccgtt cgagatcgcc tccctcttcc tgctcgtcgc
    35701 gatgatcggc gccgtcgtcc tcggcaagcg gacgatggag aggttcgact agatgatcac
    35761 cctggcccac ttcgtcggac tctcgttcgt cgtcttcacg atcggcgtcg tcggcgtcct
    35821 cgtgaggagg aacctcctga ccgtcctgat gtcgatcgag ctgatgctga acgcggtgaa
    35881 catcaacctc gtcgccttct cgaggcagct cggcgacctg accggccagg tcttctcggt
    35941 cttcgtcatc atcgtggcgg cgggagaggc ggcgatcggc ctcgcgatcg tcctctcgct
    36001 ctaccggctg aagtcgtcgg cgaacctcga ggacgccgcg gagatgaggg gctgagccga
    36061 tgagcgccgc ggacttcctc ccctggatcc cggtcttccc tctcgtcggc ttcctcgtca
    36121 acggtctcgt ctacctcgtc gcccactcga agctcggcgg aaaggacgcc ccgctcggcg
    36181 cccacggagg tggccacggc gaggcccacg ccgcgcacgc ggccggtccc gacgatcacg
    36241 gccacggggg gcacgtcgac atcccgttca aggccgtcca cacctgggtc gggaccctgg
    36301 cctcgggcct ctcgttcgtc ttcgccctcc tcgtgatcct ctcctggtgg aaggagacgg
    36361 gcggccaccg cgaggtcgtc acgaccctct ggacctggat cccgatgggg acgaacgcgg
    36421 gctggttcgg cgccccggac ttcacggtcg acgtcgcctt ccggctcgac ccgctctccg
    36481 ccctgatgct cgggttcgtg acgctcatcg ggacgctcat ccacgtctac tccgtcggct
    36541 acatggggca cgacgagggg tacgggcgct tcttcgcgta cctgaacctc ttcatgttct
    36601 cgatgctgac gctcgtcctc ggggcgaacc tggcgatcct cttcgtcggg tgggagggcg
    36661 tcggcctctg ctcctacctc ctcatcggct actaccacac gaaggacttc gcggcggacg
    36721 ccggcaagaa ggcgttcgtc atgaaccgga tcggcgactg gggcttcctg atggggctct
    36781 tcgcctgcgt cgcgatgttc gggacggtcg acttcaacgg gatgttctcg gcggtgtcga
    36841 cgaaccccgc gatctacgcg gcgggcggca tcgtcacctt cacctgcctc gccctcttcg
    36901 tcggggcgac gggcaagagc gcccagatcc cgctctacgt ctggctgccg gacgcgatgg
    36961 ccggcccgac gcccgtctcg gccctcatcc acgccgcgac gatggtcacc gccggcgtct
    37021 acatggtggc ccgctgcaac gttttcttcc gggtctccca cgagtgggcg ctgaagacgc
    37081 tcggcgaggg cgccacggtc tgggggctcc tgacgcacga cgcctcgttc gtcgtggccg
    37141 tcgtcggcgg cgtgacggcg ttcgtcgcgg cgacgatcgg cctcgcgcag accgacatca
    37201 agaaggtcct cgcctactcg accgtctcgc agctcggcta catgttcctc ggctgcggcg
    37261 tcctcgcgtt cggcgcgggc atgttccacg tcttcaccca cgcctggttc aaggcctgcc
    37321 tcttcctcgg ctccggctcc gtcatccacg ccctctcggg cgagcaggag atgacggcga
    37381 tgggcggcct ccggtcgaag atcccgcaca cctaccggac gatgctcatc gcgacgctcg
    37441 cgatcgcggg cgtgccgggc ctcgcgggct tcttctcgaa ggacctgatc ctcggcgaga
    37501 ccttcatcca ggggcacccg ttcctctggg cgctcgcggc gctcacggcc ggcctgaccg
    37561 cgttctacat gttccgcctc gtgaagatga cgttcttcgg cgagtaccgc gggccgaagg
    37621 agacgtggga gcacgtccac gagtcccccg ccacgatgac ggtcccgctc tggatcctcg
    37681 cgatcggcgc ggtcctcgtc ggctggtggg ggatcccgaa cttcatcgtc cccggcggca
    37741 accggttcga ggcgttcctc cacccggcct tccccgtgct gccgcccgac tggacgccgc
    37801 acccgcacca cgcctcgcac gcgctcgagt acggcctcat gttcggctcg atcgcgatcg
    37861 cgggtctcgg catcctcctg gcgacgacgt tcttctacaa ggggtcggcg ccgtgggtgc
    37921 tcccggcgaa ggtcgtcgcc gcgttcccga acgcctaccg ctgggtgaag aacaagtact
    37981 tcgtcgacga ggcgtacgag acggtcttcg tgaacgggct ggtcaagaag ggcggccgcc
    38041 tcctctggga ggtcgacgcc cgggtcgtcg acggcctcgt gaacggcgtg cgccacctga
    38101 cggtcggttt ctcccatgtc tcgagctggt tcgacaagac gttcgtcgac ggcgccgtga
    38161 acggcgtcgg agacaccttc caggcggcct ggaagggcta cgggaagctc cagtcgggtc
    38221 agacgcaggg ctacgccttc gtgatggccg cgggcgcctt cgccctcgtc tgcatctaca
    38281 tcatcttcgc gtgacgtgta aggacgccga atgaacggac tcctctccct cctgatcctc
    38341 ctcccgacgg ccggggcgct ggcgatcctc ttcctcccgc gggagaagac ggggccgatc
    38401 aaggccctgg cgacggccgt ggcggggctg accttcttcg cctcgctccc gctctggttc
    38461 gcccgcggtt tccgggagtc ggcgggcttc ctcttccgcg aggacctctc ctggattcct
    38521 tcgctcggcg tgaggtactc gctcggcgtc gacgggatcg cggccctcct gatcctcctg
    38581 acgacgctgc tcagcgtcat ctcgatctac gcctcgtaca cggcggtgaa gacccgggag
    38641 aaggagtact acatcttcct gctcctcctc gagacgggga tgatcggggt cttctgcgcc
    38701 ctcgacttct tcctcttcta cgtcttctgg gaggtcatgc tcgtcccgat gtacttcctc
    38761 atcggggtct ggggcggccc gcggaagctc tacgcggcga tcaagttctt cctctacacg
    38821 ctcttcgggt cggtgatcat gctcgtcggg atcatcgccc tctacttctt caacacgacc
    38881 gggttcctct ggtggaaggg gctcgggaac gccccgacgt tcgacatcct ccacttccac
    38941 gcgatcgcgc cgcagatccc gcccgacctc cagctctggc tcttctgggc gttcttcctc
    39001 gggttcgcga tcaaggtgcc gatgttcccg ttccacacgt ggctccccga cgcgcacgtc
    39061 gaggccccca cggcgggctc ggtgatcctc gccggcgtcc tcctcaagat ggggacgtac
    39121 gggttcctga ggttctcgct cccgatcttc ccgtcggccg cctccacgcg ctgggtgcag
    39181 ctgacggtcg tcgtcctcgc gatcgtcggg atcctctacg gctccctcgt ggcgatggtc
    39241 cagaaggaca tcaagaagct ggtcgcctac tcctcggtcg cccacctcgg cttcgtgatg
    39301 ctcggcttct tcgcgctgaa caacgcgggg atgcaggggg gcgtcctcca gtcgatcaac
    39361 cacggactct cgacgggcgg gctcttcctc ctcgtcggga tcatctacga gcgccgccac
    39421 acgcgtcaga tcgccgactt cggcggcctc tcgcaggtga tgccgatcta cgcgacgctc
    39481 ttcatgatca tcgccctcgc ctcgctcggg ctccccctcc tgaacggctt cgtcggcgag
    39541 ttcacgatcc tcgtcggcgc gttcctcgcc gactggaagt gggccctgtt cgccggcgtc
    39601 ggcgtcatcc tcggcgcggc gtacctcctc tggctctacc agcgggtctt cttcggggag
    39661 ctgaagcacg aggagaacaa ggggctccag gacgtgaacc tccgcgaggt cctgacgctg
    39721 gcgccgctga tcgtctgctg cgtctggatc ggcgtctacc cgaagccgtt cttcgcgatg
    39781 accgcccccg cctcggcgaa gatcgtcgcg gccctcgacg cggcgaaggg ggcgccgccg
    39841 gccctggccg ccgcgctctc cgcggcgccg acggcgccgg tcccgtccgc caccccggtc
    39901 gtcccggccg gcgctcccct ccccgagtcg gctcccaggg ggaactgacc cgtggccctc
    39961 ccgttcccgc tctccgacct cctggtcatc ctgcccgagc tgatcctctc cgagctcgcg
    40021 ctcgggatcc tgctctgggc ggccgtcgtc aagagggagc gggagagggc gctcgcctgg
    40081 gcgtcgctcg cgtcgctcgc gatcgcggcc gccctgctgc ccgtcgcggc gtcgttcgcc
    40141 ggggagctcc cccggtcggc gttcggggga atgatcgcgc tcgacggctt ctcgatcttc
    40201 ttcaaggcgc tgacgctcct cgcggccgcg gtcacgatcc tcttctcgct ccgcttcgtc
    40261 gggacctcgc cctaccccgg aggcgagtac tacgcgctcc tcctcctgac gacggtcggc
    40321 atgatgttca tggcgtcggg gacgcatctc gtctcgatct acatcgccct cgagctgatg
    40381 gccctctccc agtacgtcct ggcgggctac ttcaagcagg aggcgaagtc ggtcgaggcc
    40441 gcggcgaagt acttcgtcct cggggcgttc tcctccggcg tcctcctcta cggcctctcc
    40501 ctcgtctacg gcgcgaccgg gacgctctcc ctcgccggcg tctcctcggc cctcgaggcc
    40561 gcgccccgct cgacgctcct catggtcggc gtcgtcctcg tcgcgtgcgg gatgctcttc
    40621 aagatcgcct cggcgccgtt ccacgtctgg gcgcccgacg tgtacgaagg cgcgccgacg
    40681 ccggtctcgg cgttcttcgc ggtcggcccg aagctcgcgg cctacgccat cttcgcgcgc
    40741 gtcttcttcg acggcttcgg accgcgatcc gacgactggg cgcccgtcct cgcggcctcg
    40801 gcggcgctca cgatggtcgt gggtaacgtc ggtgccctga tgcagacgaa cgtcaagagg
    40861 ctcctcggct actcctcgat cgggcacgcg ggctacgcgc tcctcgggct cctcgcctac
    40921 cgggcggggg gcgccgcggg ccagcccgag gcgttcggcc tctgggcgat cctcttctac
    40981 ctgaccgcct acctcctgat gacgttcggg gcgttcggcc tggtcgtcct cctcgaggcg
    41041 aagggctacg cgacggagag cgtcgacgac ttcaacggcc tctggaagcg gaacccgctc
    41101 gcggcgggcg cgatggtcgt cttcctcctc tcgctcgccg gcatcccgcc gacggccggc
    41161 ttcgtcggga agtacctcct cttctcggcg gcgctgaagg cgggctggcc cgccctcgtc
    41221 gtcctgggcg tcctgatgtc ggccgtctcg ctcttctact acttccggat cgtccgggcg
    41281 atgtacctgg tcgacgggga gggggaggtc cgctggcgcg aggagccggc cgtggccgtc
    41341 gcggtcggcc tctgcttcgc cgggacgctc gcgttcggag tcctccccca gccgctcatc
    41401 gccctggcga agtcctgcct cctgccgtag ggctgcgatg cccgagccgc ggagggagcc
    41461 cgacccgatc ctccgcctcc tcggcgacgt cctgagcttc ggctgggtcc tccccgcctg
    41521 cctcgcggcg ggcggggggc tcggctggct cgtcgaccgg tggctcggga gcttcccggc
    41581 cgccaccgcc gtcctcggcc tcctcggctt cgccggcggc atccggcagc tcctgcgcga
    41641 agcggacagg ctcgaaggcc ggcggaagga cactcctggg tccgggccgg ggccggccgg
    41701 ggacgggacg tgagcctcgc gctcctcccc caggaacggc gggtcttccg ggtggccgcc
    41761 tgggccgcgc tcctcgccgc cctcgcgctc ggagccctcg ggaggttccg ggaagccgcc
    41821 gccttgacgg ctggagcggc cgtagctatc gtctccggcc tctggttgtc ggacgtggcc
    41881 gggagactgc tcgttccccg tccacgggcg tcgacccggc tcgactggaa gttcgcgctc
    41941 cgcgctctcc tccgctatgc cttcctgggg ctcgcggcgt acggctcggt tcgcctcttc
    42001 ccggacgagg tcccgtggct cctcggaggt ctctccgcgg tcgtcctcgg ggtcctcgcc
    42061 gacgggttcc ggggaggcgc ggccggccgg tccactccgg aggaagagga agaaggaacg
    42121 accccgcggg agtgagatgc agcaccatca ctcgatcctg ttcggacccg tcaacgcgct
    42181 cctgacggcg atcttcggat cgcccgagaa cgcgccgtcg tggtggctcc acggagtgac
    42241 gatcggcggc gtccacgttg cgggcgtcgc cccggtcgac cacgcgaccg gggccgtcct
    42301 cggctggatc cccgatcacg tgatcatggc gctcgtcgtc ctcctcgtct tcgcggtcgt
    42361 cctgccggtc ctcgcccgcc gcttccggga gggcgttccg ggcggggcac agaacgtcct
    42421 cgaggtcttc gtcgacttcc tccgcggcct gatcaaggag aacatccccc acaccggcga
    42481 gaagtacctc ccggtggtcg gggcgttctt cttcttcatc acggtctcga acctggtcgg
    42541 ccagctcttc ttcctccagc cgccgacctc cagcctcaac acgacgttcg ccctctcgct
    42601 ctcgtgcctc gtcttcttca acgcggccgg gatcaaggcg cacggcgtct tcggctacct
    42661 gaagacgttc gcgggcccga gcgcggcgct cgcgatcctc gtcttcccga tcgagatgat
    42721 cggcaacttc gcccgggcgc tctccctggc gatgcgtctc ttcgggaaca tctacggcga
    42781 gcacacggcg acgaaccagt tcgtcgagct cgtgccgctc ctcgtgccga tgccgatgaa
    42841 cgccctcgga atcttcgggt cgttcctcca ggcgtacgtc ttcaccctcc tgaccaccgt
    42901 ctacatcggg ggcgcgatcg cccacgagca ctgaaacgcg gaacaccgaa cccgaaagga
    42961 gcccaaagga catgatcaag aagcttctcc ccgccgtcgc cgtcgccggc ctcgccttcc
    43021 tgctcccgaa cctcgccttc gcgcaggagg ccgcggaggc cgccaaggcg acgagcggtg
    43081 gcgcgggctg gggccacctg tcggccgcgc tcgtcctggc gatcggcgcg accgccggcg
    43141 ccaccgcgca gggccggacg gccgtcgccg cctgcgacgg catggcccgc aaccccggcg
    43201 ccgccggcca gatccgcggc atcgcgttcc tcggcctcgt cctgatcgag tcgctcgtca
    43261 tctacacgct cgtcatcgcc ttcctcctcc aggggaagtg acccggcggg cccgcgcggc
    43321 ccggaccggt gcggagggcc ccggcgcctg ccggggcctt gccgtatcgg ccgcaatccg
    43381 gcgggcccgc gccgtcgttg aagggagaag atggccctct tcggattccg ccgcgaccgg
    43441 gccccgaacg cctgggacct ccgatcgggg gagctgccgt acgcggacgc cctcttcggg
    43501 accgcccttc gcctgacgag gaaccggcag gacgcggagg acctcctcca ggagacgtac
    43561 ctgcgggcct tcgcccacta cgacagcttc cgcgagggga cgaacctgaa ggcgtggctc
    43621 ttccggatcc tgaagaacgg gttcatcaac cgctaccgcc agcagaaggc ggggccccga
    43681 gaggtcgacc tcgagcgcgg aggggcgacg ttcgagtcgg ccctcgagga agtggtccct
    43741 ccggccgcga cgcccgagga cgagctcctc ggccgttcgc tcgacgggga cgtcgcgcgc
    43801 gcgctgacga gccttccgga ggacttccgg ctcgtcgtcg agctcgtcga cctccaggac
    43861 ttctcctacc gcgaggtcgc ggacatcctc gagatcccgc tcgggaccgt catgtcgcgg
    43921 ctctatcgag gtcgtcgcct gctcgaggag gcgctccttg cgtacgggaa gcgccggggc
    43981 tacttcggag cgacggcttc gccggcacgc agccggcgtg cggccggaga ggccgccggg
    44041 gcggcggaat acccgccacg gcgtcggcgt tagacctcgc tggagaggcc cccgcgggcc
    44101 gcgcgatgga ctgcgaaacc gccagccccc tgatcgaggc gctctgtgac gacgagctcg
    44161 acgtcgcgac ggcggcccgc cttctggcgc acctcgatac gtgcgcgcac tgcgccgctc
    44221 tccggcgcga cgccgagacc cggaacgcgg ccctccggga ggcgcggccg gacgaccaga
    44281 tcccggaggc ggtccggacg cgcctcgccc gcgcggtcgc gcaggcgccg gggccgcgtc
    44341 ggacccggcg ccggggattc ctcgccgtcg cggcggccgc cgcaggggcg gcggcggtcg
    44401 gggcggtgct cttcgttcgg agctccccgc cgccggcgcc cacgctcgtc gaggcgtccg
    44461 aggtccggga gctcaccggc gaggtcttct gcctgcgctg cgccctcgcc cggctcttcc
    44521 cggagactcc ggtcctcgac ccccgccacc tcccggtcct caggaccgac gacggggagg
    44581 tcgtcacgct cctcgacggc gctgtgacgg aggcggtcct cgcgcggaag ggctgctccg
    44641 gccgccgggt cgcgctgacg gtccgcctgt tcccgcggca ggagctcgcc gaggttctcg
    44701 aagcgcgcga gctggggccc gcgtcgggcc cggttccggg cgaggtcgtg gcgacggcgg
    44761 tccggcgctc ctcgtcctcc ccgccctgag cggcctggtc tagactgacc gtcgtggcgc
    44821 acgaggagcc cgaccgcttc attccgatcc tctccctcgt cgcggggctc gcggccttcg
    44881 tggccggcgg cgcggcatcg gtgtcggccg cgtgggcggc cgctggcttc ggtaatccgt
    44941 tccggcagac tctcgtcctc gacctgggac tcgccgcctc ggcggtcgcg gggctgctcg
    45001 ccggcgccgc ggtcgcggtc ttcctccagc gtcccgcggc ccgcctcggc cgaaccctgg
    45061 ccgcggcccg gcagatggcc gaaggcgacc tcgcctcgcg agccccggag ggacgcgacc
    45121 tggcgggacg gatcggccgg ttcctgaacg cggtcgccgg ccggggcgcg cacctcctgt
    45181 cgtcggttcg tcgggagcac ggacagctca accggcaggt cgcggtcctg cgcgcggcgt
    45241 cgatccgcaa ccgcgagcgg gctgcccagg aacgggtgcg gctctcgagc gccaccgccg
    45301 ccgtcggcgg gctggacggg acgatccgcg cgatcgccga gagcgtcgag acgctcgcgg
    45361 cgggctccga ggagacggcg gccgccgtcg cggaggtgga cgggtcgctc acgcagctcc
    45421 tcgcgcgatc ggaagggctc ctcgccgact cgacggacgg ggccggggcg gctgcctccc
    45481 tcgccgaagg cgccgcggtc ctggacgcga cgctcagcga cctcgcgcgg cgggcggacg
    45541 acctcgccga gggctcgact caggccgaga aggcgcacgg cgcggttctc gcgtcggcgc
    45601 gcgaggcggc ggagcaggcc gcgcacgcgg ccaccgaggc ccgctcgggg cgggccgtcg
    45661 tcgaggaggc ccgcgcttcc gtcgcggcga tccgcctctc gtccggtacg gtccgtgacg
    45721 cggtcgggcg tgtcgaggcg cgctcccgcg aggtggggcg catcctcgag atgctcgagg
    45781 acatcgcgcg gcagacgaac ctcctcgccc tgaacgcctc gttcctcgcg acgcgcgcgg
    45841 gggagcacgg gcgcggcttc gccgtcgtcg ccgccgagat ccgtcgcctc tccgagcgga
    45901 cctccgaagg ggcccgtggg atcggcggcc acgtcgcggg aatgcgggaa gaggtcgacg
    45961 cggcgcgggc ggcggccgag gaggagacgc ggctcgtcac ccgcggcgtc gagacggcga
    46021 cgcgggcggg tgaggcgctc gccggcatcg aggccgcggc ggcgcgggcc gaggaggccg
    46081 tcgccgcgat ccgcgaggtg tcgcgcgccc aggccgcgac ggtggcggcg agcgtcgcct
    46141 cggtcggcga gatgcgcgag gggctccggg cgctcgcgga ggaaggacga cgcaacaccc
    46201 gcgaggcgga gcggatccgc gagctcgtcg ggcggatccg ggagctcgcc ggcttcgtcg
    46261 agcggaccgt ccaggagcaa aagggggcgg cgggccagat cgcggtcgcg gcggagcgct
    46321 ccctcggcct catgcgcgac atccaggacg ccgtcggccg ccagacggct gacagccacc
    46381 gaatactcgg cctcctctcc gaggtcgagg cgatgagcct cgacaccctc gcgtccgccg
    46441 cttccgtcga cgacacgacg gcggcgctcg agaccctcgc cggctcgctc gaggacgagg
    46501 tcgggcggtt ccgggtcggc gcggagcttc aggccacgtg acgcttcgcc gcgtcgtcgc
    46561 gttccgggcg ggcggcgtgg cgtgcgcggc cgatcccgcc gacgtcgagc ggattctccg
    46621 tcccgcgtcg ctcctcccgg ccccgggggc gccctcctgg atcgcggggc tcgtctcggg
    46681 gccccgggga ctcctcgccg tcgtcgacgt cggccgccgc ctctttccgg gacgggacac
    46741 ccccggaccc cgcgacctcc tcgccggaac gttcgacgga atccccgcgg cgctcggcgc
    46801 cgaggtcgtg gagggaatcc tgacggtgga cggggccgag gggctcccgc cggaggggct
    46861 agcgccggcc gtcgcggact gtctgaaggg agtggtcgtg gccggaggcg agaggcgcgt
    46921 cctcctcgac gtccgccggc tcgtggcggg cgagccggcc cgcctcgcgg ctcagatcgc
    46981 gagcgaagcg acggagcgcc gggaccagtc ctcgtagagg agaccgagga cgacgacgtc
    47041 gtgcctctgg ccgtgcgcga agacgcggct ccggagcgtc ccttcctcca cgaagccgag
    47101 gcgcttcagg acggcgcgcg cggcgacgtt ccccgccgcg acgaaggcct cgatccggag
    47161 gaggccgcgg tggaggaaga gataggagac gagggcgcgg tgcgcgtccg ttccgagccc
    47221 cttcccgcgc tcggccggct cgaggatcgt ggcggtgatc cgggcgacgc gggagtgcag
    47281 gtcgacgtcg tcgacaccgg cgagcccgac ccggcgtccc gcacggtcct cgatgacgag
    47341 gcgcgtcagg tcctccgaga gcaggccgtc ctgctcgaac cgccgccgga ggctctcggc
    47401 ctcgccgacg aagaacggct cgaactcgcc gaaggcggag ggcttcccgc aggcggcgac
    47461 gagccagtcg aggtcgtccg ccttcaccgg gcggagtcgg acggaggccc cggccagcac
    47521 tacatcctct ccgggatcgg gacgccgagg acgccgagga cgtcttcgag cgtccgggcg
    47581 acgagctgga gcgcggcccg ccgggcccgc cggctctcgt cgttctccgc ctggaggatc
    47641 gggtgcgtgt ggtagaggtg gtggaaggcg gcaccgacgg cgagggcgtg ccggaccagg
    47701 agcgacacct cgaacgtctc ggcggccttc tcgaacgtct cctgcgtccg cccgcaggtc
    47761 ctcacgacgt cccagaggcc gtcgtccagg tgcgcggcgt cgagcgctcc gacggaggcg
    47821 gcctcctccg cgtcgaggag acccgggagc cctttctcct ccagcttgcg gaagatgttc
    47881 gccgtcctca cggccgcgta ctgaaggtac ggtcccgtgt ccccctcgaa cgcgagcgcc
    47941 tcgtcgaagt cgaaggcgat caccttgttc cgcgagtagc gggtcatgta gacgcggagc
    48001 gcgccgatcg cgatcgcctc ggcccgggag aggtcgggac gctcgcccgg ctgccggtcg
    48061 aggatctgct cgatcgcctt ctcgacgagc cgctcgacga ggtcgtcggc cttcacgccg
    48121 agccccttgc ggcccgacat ctcgacgtag gcgcggccgc ggtcttcgtc cgagagggtc
    48181 acgccgagga gctcggccga cgccggcgtc agcgcgacca tctcgtacgc gaagtggacc
    48241 gaccggtcgg cgccctccgg gaagccggcc cggcggaccg cctccttgac gaccttctgc
    48301 gggtacgact gacgcacgtc gatgacgttg atgacggagt cccctccgcc gaagcggccg
    48361 cgcgcggagg ggtccgcgcc ctcggcgtcg tggcgggtcc ggtagagcgg cgacgtcccg
    48421 tcggaggcga agaactcggc ctcgccgaac gggttcacgt agggcgcctt ctcgtactcg
    48481 aagtcgatcc cgaggtcgcc cagcttccag agctggtagg cgacgtcctt gcccgtgtag
    48541 gtgacggtgc cgttcgagcg gacgaggacc ttgtcggcgt cctccatccc ctcgaactcc
    48601 ttcgccccct cgagcttcat gacccagcag cccgcgttct tcccctcggt ctcgagctgc
    48661 gttgcgccgg cggccttcag gaggtcgaac gccttctgcc agaagcggcg gccgaggatg
    48721 tcgctctcgc gcgggaggag gtcgtaggag acgccgatcc tctccatcgt cttcaggtgg
    48781 cagcggacga cggcctccgc gaggcgggag gcgagggcgg ccgtggcccg agcctccccg
    48841 gagagctcgt gtccgagggc cgccccctcg atggcgtgga gcgtcgcctt ccggcgctcc
    48901 tcgagcgacg ggtcggcggc gtagctccgg gtgacgaggg cgtagaggtc ccacgtcagg
    48961 tcgccgagcc ggcgcggctc ggtcccgggg gcgtcgtcgg ggaaggcgcc gaggacctcg
    49021 cggatctccg aggcgagctc ctcggcccgg gtcggctcgg cgagctcgtc ggcgtgctcg
    49081 aggccgacga cgacgtcggc gacctggacc cccgtgtcgt cgagatagtt ctgcacctcg
    49141 acgtgcttcc cgaggcggcg gaagaggtgg acgaggacgt ccccgaggac ggcgttccgg
    49201 agatgaccga tgtgggcggc cttgttcggg ttgatgttcg tgtgctcgac gatgaccttc
    49261 ccggacgcct tcgccgcctc gggcgcggcg acgagcatcg cggcgagcgc cttcggccgg
    49321 tcgaggaaga ggttcacgta gccgcctccc tcgacgctcc ggcgggcgac gatcgaaggc
    49381 agctcgagcg ccggggcgag ctcctcggcg atcttccggg gcgggcgtcg gaggaccttg
    49441 gcgagctcga acgcgatcgg gagggcgagg tccccgagct cgaccttcgg gggcgtctcc
    49501 aggacgagcc ggggcggctc gacgtcccag aggcgacggc aggcatcctt cacggcggct
    49561 tcgacatggt tcttcaggcc tgggatcacg gcgacctcgc gaaattgcgt ttgaaggcgg
    49621 gacgcgggag cctcccgcgc ccaaacctcg attcttgcac gccgggcgct ccggagccga
    49681 cggggccggg gggccgccac cggcgctggg ccacccccgc ggcgtggcgc cggacgaccg
    49741 ggcgaatcgg gacttgaacc cagacgcggt tgcgggcgta atcagcggta ccgtgaccag
    49801 accccccgtc cgaccctggc ggagcggcgc cctcctcgtc ctcgtcctcg gggcgttcgc
    49861 gggaggctgc cggacgaccg acgagacgcc cgtcctccgc ctcacgccgg agcggtggcg
    49921 cgaggccgtt gccgcccgcg gcgtcgaccc cggggatgtc ccgatgccga tgatggtcac
    49981 gccgcagatc cgggccgcgg cggagcagct cgccgggccg gcgggcgacg acggcgagag
    50041 gctccgccgg ctgcaggcgg ccatcctgga ccggaagagc tacgcgttcg agtacgacac
    50101 cgtggcgacg ttcaccgcgg cggaagcgtt cgcgttccgg cgcgggaatt gcgtttcgtt
    50161 cacgaacctc ttcatcgcct tcggccgggc cctcgggatc cccctccagg cggccctcgt
    50221 cttccggcgg gcccggagcg agaaggaggg cgacctcgtg atggtctaca accacatggt
    50281 cgccgtcctg ccgaagctcc ggcagctgac cgtcttcgac ttctacatga cccgcgacga
    50341 gtcccgggtc gacctgaggc tgatcgatga cctggccgtc gccgcgatct cggcgagcaa
    50401 ccggggcgtg gaagccctcc gcgcgggaga cctctcccgg gcccgcacgc tcctcgagag
    50461 ggcgacgaag ctgcagcgct ccctcccgga cctccactcg aacctcggga tcgtgaggtg
    50521 gaagcagggc gacatcgagg gcgcctacga agcgttccgg gaggggctcg acctcgaccc
    50581 ccaccgaccc gccctcctgc acaacctggc ggcgctctac atcgagcagg ggagggccac
    50641 cgaggcgcgg gccgccctcg cggccgcgaa cacccgggac gcctcggcct acctcttcgt
    50701 cgtccagggc gacctggagc tcgcgggcgg gaacttcaag caggctcgga ccgcctacaa
    50761 acgggcccac cgcgaaggga aggacctcgt cgaggcgctc ctcggcctcg cgcgcgtcga
    50821 gcgggcgctc gggaacgagc ccgcggcgca gcgtgcgctt cggaaggcgc tgaagatccg
    50881 gccggacgac ccgcaggtcc aggcgctcct cgggagcccc tgaccgggcc ggctttccgg
    50941 cgcgacgccc ccgagcgcgg tgcgatatag tcccaaggat gcgccggtcc cggcgaaccc
    51001 cccctgaaag gaccacacgg atgagaaagc tccggcttct cgcgaccctc gtcccgctcg
    51061 tggcgctcgc gctccccgcg gccgcccagc tggataagtt caaggactgg gagagctccc
    51121 ccgagttcgt ctactacgcc acggaggacg agaagaaggc ctgggccggc gtcacgaacg
    51181 acgaagaggc ccagaagttc ttcaacctct tctggggcaa gcgccacccc gactaccaga
    51241 agaccgccca gaacgtcttc cggacgaagt tcgacgccct cgtcgccgag gccgacaagc
    51301 gcttcaccct cgggaagaag cgcggcgccc tgaccgagcg cggcaaggtc ctgatcctcc
    51361 tcggcccgcc gaaggcgatc gcctcgaagg tcgagtcggc cgacgccggg tcggccggag
    51421 cggagggcga ggcgggctcc ttcctgtccc gcggcaacgt cgtggtgatc cagttccagt
    51481 acgacaagga gcagctcccg gagtgggccg ggatgaagtc gttccgcgtc aacttcacgg
    51541 tcgacgagcg ggccgagcgg gagacgcccg acaagaccgc cgacatcaag aagctctgga
    51601 agaaggcgat cgcggccgcc gtcgtcaacc cgaagatgac ggagccgccg gtctacaaga
    51661 cgcgcgagga gtacgaggcg gagatgaagg ccgcggccga ggccgccgcc gaggccgcaa
    51721 agggtccggt cctctcggcg cccgtccgcg cttcgctcga agcgctgctc gcgaaggagc
    51781 cccagggcgg gctctctctc ttcccgctcg cctacggcga caaggcgacc cgcctgatgg
    51841 tccagctcta cgttccggcc tcggtagcgc ccgcaggcgc tgccggcgcg gccccggagg
    51901 gaatgaaggt cgccctcctc gtccgcggca aggacgggaa ggacgccgcc cggcgcgagg
    51961 agcccgcggt cctgcagaag gtgcgcgagg actggttcgt cgacgtggct ctcccggtcg
    52021 acgccggtga gtaccaggtc gcggcgctgc tcctcgacgc gtcgggcgcc gagaaggtct
    52081 cggcccaccg gcccgtcacg gtgactccgc tgccgtccga gctcgcgatg tccccgctcc
    52141 tgctcgcctg caccgacatc gccgctgacg gagcgaaggc ggaggagccg ttcgtcttct
    52201 cggtccggaa gttcgtcgcg aggggcggcg acgtcctcga gaagaccgac ggtctcgcct
    52261 acgtggcccg ggtctacaac cccgcggtcg accccgcgac gaagaagctc cacctgaaga
    52321 ggtcgatctc gatcaagccg aagaacggct ccacgatcga cgtgccccag cccgcggacg
    52381 agccgatgat cgtgccggag cagaccggct cggcgaccgc cctggtcatc gatctggccg
    52441 gctccatcgt cgacgtcaac ctcggcgact acttccgccc cggcgagtac gtcatgacgc
    52501 tgaagatcac cgacgtcatc tccggcaaga gcgtcgaggc gaaggcgccg ttcacgctcg
    52561 cggcgccgcc ggccccggcg aaggcgccgg ccgccaaggc cccggctccc aaggcgccgg
    52621 ccgcgaagaa gtagcgcctc ccgcacgccc ccgtacgatc gacggccccg gcgaaagccg
    52681 gggccgtcgt gtttcgggga gcggcgcgcc gacgggcgtt cctagtcgac gcggacgtcc
    52741 gcggacgcga gcgccatccg gccggtggcg acgtcgcgca cgcctgccac gaagcggatg
    52801 ttcccctttc cgctcttgac ctggccgcgg tagacgaacg gctggctgct ggcacgctcc
    52861 cactccgaga ccgggatcga gagcgtcttc ttctccgggg tgacgtcgga tcgggcgccc
    52921 gtgtcctgga tggcggcgag ggtgatctcg acgaccgccg tcttccggtc gccttcgtcg
    52981 cggaaggtca ggtccgcgag ggagaactcg acctcgtacg gacccgtccg ccgggcgagc
    53041 ccgcccgccg gcgagaggct cccgatccga agcgccgccg ggaagtcacc ctggacgccg
    53101 ggcgtcagga gggccatctc gatgcggtcg acggccgcct cgtcggcgct ccgggggacg
    53161 aagtgggtgc gcgcgcgaac gaccacgccc ggccgggtcg tccgcacctc gaccttcctc
    53221 tcgcccgcgc cggcgaggcc ggcgagcgtg accccgaccg agtagtagcc gctcgattcc
    53281 cggtagacgc ggtcgaggtc cgcccggaac tcgttccggt tctcgacgag cgtcccgccc
    53341 gtacccgtcg ccagcgtcac catcgagtca cggaaaccct gcgcgcggta gaacgggctg
    53401 atcgcggggt tcgaccgctc ggaggcgtcg gcgccggcgc cgagcccctt cgcgtcgaag
    53461 acctggatgg tgatccccgc gccgttggcc tcggcgagga ccttctcgat ctcctcgccg
    53521 acgtcttcgg cgaactgcga gaccagtctc ggcccgtagg cgagggcaag ggcccgtccg
    53581 gggtgccatt cgtacccgtt cgtcaccagg aggagcgttc tcttccccga gcgggcggcc
    53641 agggccgaca cgctccgcgc gagctcgcgg agggcccacc gctcgcggtg cgcggcctcc
    53701 gtcgaccagt tacggatcag ggaggcccgg gagcgaggcg agatccggat ctcccgcacc
    53761 gtcctctggt actccgactc gagcgcgagg cccctgggcg cgacggcctc cagcttcgaa
    53821 aggccgccga ggaggtcctc cttgctgctc gtgaagggga cgaggacccg ggtcgccccg
    53881 ttgtaggaga cgatcgaggc ccggtcggac ggcgagagcc gcatcacgaa gtcccggagc
    53941 ccctcgagga ccggcttgcg gtcctgcggg aggagccgct cgtcgtcgaa atagacgagg
    54001 aactgcctcg ggacgtaggt ctctcccgcg tcgttgcgaa gggtgtcgag gacgaggtcc
    54061 ggcgaggcgg tcgcgaggtc gggcccgtgg acggtgccgg cctccacccg ggcgaagtag
    54121 tcgatcggga cgaccttctt gtcgatgcgg acgacgaact cgtcccgggt caggtccgtg
    54181 accggccggc cgtccttcgt cgcgacgacg tcgaggtcca gcgcgcggac ctccacggcc
    54241 gcgcgtactc cgaccgggag cggctcctgc ggcggctcct cgccgcgcat cgcggggctc
    54301 ggagagagga cgagggcgaa tgcgagaaga gcgagtgaag cggttcgggc gaacatggga
    54361 tacctccgtt cggagctgac cgggactgcc aggtactacg gcgggagccg cgcggacgga
    54421 tcagagactg aggagctcgc gaagggccga ggcgtcccgg ccgatccggt ccgccagcgc
    54481 ttcgtcgacg gcgagggagg cgtaccggtc ggcgagctcc gggtcgtcgc tcgtccactc
    54541 gcggccgacc tgctccgccg cgaccatctg cttcgcgaaa aagatcagct gctcggcgac
    54601 ggagctccgg ctcgagacct gaccgtccga accgaggacg tcggcgaacg cgacgggcag
    54661 attcagcttt cgtgccagcg cacggccgct tgtcgcgcgg taccagtcga gggtcgcggc
    54721 ccggaacggt ccggggtcga agtcctccgc gaggagcagc ttgaggagct cgtagccgcc
    54781 caccggctcg aggagcgcca gggaccaggc gagcccgggg tcctctccga gcgacggggc
    54841 gacgtgggat gcggccacgg ccgtggacac ggcggcgctc cggagcgccc tctcgaggcg
    54901 atcccgcccc gagaccaccg cgacgcccgg ggcggtgagg agctcgaggg tccgccggag
    54961 cgctcccggc ccgatcgcgg agagggcgcc gtcgacggtc gacaggagtc ccgtggccgg
    55021 agtgcgaagc gcgtttccga cgaagaggag gtcgaccgtg agaaacgggt ccgtcgcggc
    55081 gaggcggaac gtctcctcgc cctccgtgcc ggacgcgagg gcggcgcggc atcgttcgac
    55141 ggtgtcctcg aagagcggga agggccggaa cggcttccga agcgaggccg cgaccttctc
    55201 cgagaacctc ccgaagagct cgaagcgctc ctgccacacg gacgggggcg ggctctcgag
    55261 gtcgagcccc tcgacgacgg agaagaacga gcgcgcgacg cggcgtgccg agagctcgtc
    55321 gccctccgcc tccggctcct cgaggtcctc cgagcggacg gcgcgaatgc gggccgtgtc
    55381 gtcgacggcc cgcgcccctt cgagcgccag gagctggccg cgcagcgaaa gctggatccg
    55441 gaggaggtcc tcggtgacga cggtggggtc gtagacgaac cgggcgccgc gccagtggaa
    55501 gaggtcgagg aggaccttca gcgcgagctc ctcgacggcc ccggccagcg cctcgcgcga
    55561 aagcaggccc gtctcgaaga cgagcctcgt gaggctccgg cccgtcagga agctgtcggc
    55621 gaggagctcg tagacgaccg gttccgggag gacgccgcgc gcgaccagga acgtcccgag
    55681 ccgctcctcc ggccggaccg acgagacgaa gacgatcgcg ccgtccttga cgtcgatcat
    55741 cctccggtcc tccccgcgcg agatcgtcaa tcggccggag agccggttga tctcgatcca
    55801 ctgcaggagg tcggcgaggg cgaaggtatc gagcgttccg ctgagcgact tcgacaaggc
    55861 tgcacgaagt gtgacacgaa gcgggtccgg agggagccgg ccgtcgcacg cggaggcggc
    55921 cggccgggcg gtcgcccctc cggcgcgcgt gattcgagat gcggtctcgc acggtgtcct
    55981 cgcgtgccat ttccattagc atcggcctca tgtccaccac gaagaccgac accgtcacga
    56041 cctcagggat cccggtccgt ccctcctatg ccccggggga cgtcccgcag ggctgggatc
    56101 cgggcgtgcc cggtgcgttc ccgtacaccc gcggcgcgat ggccggcggc taccggaaga
    56161 agctctggac gatgcgccag tacgccggct tctcgaccgc ccgcgagtcc aacgcccgct
    56221 accgctacct cctcgcccag gggcagaccg gcctttccgt cgcgttcgac ctcccgaccc
    56281 agatcggctt cgactccgac gccgcgctcg cgcgcggcga ggtcggccgt gtcggcgtcg
    56341 ccatcgattc gatcgaggac atggagaccc tcctcgacgg catcccgctc ggcgaggtgt
    56401 cgatctcgat gacgatcaac gcgaccgcgt cgatcctcct cggcttcctt ctcgccgtcg
    56461 cgcggcggcg cgggacggag tggaaggcgc tgtcggggac gatccagaac gacatcctca
    56521 aggagtacgc ggcccgcggg acctacgcgt tcccgccgaa gccgtcgatg cgcctcatca
    56581 ccgacatctt cggcttctgc gcggagaacg tcccccgctg gaacaccatc tccatctcgg
    56641 gctaccacat ccgcgaggcg ggctcgacgg cggtgcagga ggtcgccttc acgctcgccg
    56701 acggcgtcga gtacctctcg gccgcggtgc gggcgggcct cgacgcgaag gcgatcgccc
    56761 cgcggctcac cttcttcttc aacgtccaca acgacttcct cgaggagatc gcgaagttcc
    56821 gggcggcgcg gacgctctgg gcgcgcctgc tgaaggaccg cttcggcatc gacgacccgc
    56881 gcgcgcaggc gctccgcttc cacacgcaga cggcgggctc gacgctcacg gcgcagcagc
    56941 ccgacgtgaa cgtcgtccgc gtcgccatcc aggcgctcgc ggccgtcctc ggcggaacgc
    57001 agtcgctcca cacgaactcg cgggacgagg cgctcgccct gccgacgacc gaggcggccc
    57061 gcctcgcgct ccggacgcag caggtcatcg cgtacgagac gggggtcgcg cgggtcgccg
    57121 acccgctcgg cggatcgtgg gcgatcgaag ccctcaccgc ggagatcgtg gagaaggcct
    57181 cggcgctcat cgggaggatc gacgagatgg gaggggcgct cgccgccatc gaggcgggct
    57241 acttccagcg cgagatccag gaggcggcgt accaggcgca gaaggcgatc gagtcgaagg
    57301 gccaggtcgt cgtcggcgtg aacgagttcg tcctcgagga ggagaacccg atcccgacga
    57361 tgtcgatcga cccgaaggtc gagacggagc aggtcgagcg gctcgccgcg ttccgcgccc
    57421 ggcgcgacgg cgcgaaggtc gaacgcctcc gtgccgacct cgtcgccgcg gcgaaggagg
    57481 ggcggaacct gatgccaccg atcgtcgagg ccgtcgacgg gaacgtcacg cttggcgagg
    57541 tcgtcgagtc gctgaagtcg gtcttcggcg accacaagga cacggtcagt ctctgagcgg
    57601 gcggcgccgg cacccgggag cccctcgggg gctccgggtg ccggccggcc ccggaaaccg
    57661 gccaccggcg gtcctcgctt cgcggcaaaa ccgctgccag aacgaacccc ctcctgtgta
    57721 aatcgtttcg ggccgctccg gaagcggctt cacgcggacc ttacatttct cgcgtggtgt
    57781 caagtccctg tggtgcagat acttgacgtg cgtgcaccgt ccggagtgca ttccgtgcgc
    57841 ggcccgcttc cgatgcggct tcctggactt ttcgacagcc gaatccacag gcttatgcac
    57901 agctcgtctg ggaaaggcgg taagcctctt tgctggaagg tcttcggagc cgtcaggcgt
    57961 tctcttcggg gaacggaagg accccgtcga cctcgacgac gcccgggtcg aagcaccgtc
    58021 ggcatcgggc ctcgtacatc ccggccgcgc cgacgacgac ccgttccttc gaggcgacga
    58081 gccgctgggt gcggctcgcg ggacctccgc agaggatgca gatggcccga gtcttcacga
    58141 cttcctcggc gagcgccatg agcgtcggca tcggctcgaa cggccggccg aggtagtcct
    58201 ggtcgaggcc ggcgacgacg acgcgcttgc cgaggtcggc gagccggctc gcgacctcca
    58261 cgatcccggc gtcgaagaac tgcgcctcgt cgatcccgac gacttccgtg cggtcttcgg
    58321 tgcgggccag gacctcggcc gacgtcgcga cggacagggc cgtgagccgc atccccgtgt
    58381 gcgagacgac ctcgacctcc gagaacctgt cgtcgagctt cggcttgaag acctggaccc
    58441 ggcgccgggc gatcttcgcg cgggtgacgc gacggatcag ctcctcggac ttgcccgaga
    58501 acatcgagcc gcagatgacc tcgatcgagc cgcttcccgg gagcatcaac gtctcctccc
    58561 cgcgcgcgcc gctccgggct tccgcgatcc cggcgtcccc tgcctgtcga cgcccggctt
    58621 cgtcgcggac gtcacgccga ccttcgggca gatctcctcg aggaggcagc cggagcagcg
    58681 cggcgaacgc gacgtgcagg tccggcgacc gtgaaggatg agcgcgtgcc ccgcgaagac
    58741 ccactcctcc cgcggcagga gcgcgttgag gtcccgctcg accttctccg ggtccgtggc
    58801 gcccgaccag ccgaggcgcc gcgcgaggcg gccgacgtgc gtgtccacga cgactccgtc
    58861 gggagcgccg aacgcgttcc cgaggacgac gttcgcggtc ttccgcccga cgccggggag
    58921 cgcgttcagc tcctccatcg tccgcggcac ctcgccgccg tgctgctcga cgatcgcccg
    58981 gcaggcgccg cggagggagc gcgccttggc gttgaaaaag cccgtggagc ggatcagctc
    59041 ctcgagctcg gggccggccg cggccatcgc ggccgccgtc gggagcgccc ggaagagagc
    59101 gggagtcacg gcgttcacgc gcgcgtcggt gcactgggcg gagaggatcg tcgagacgag
    59161 gagctggaag gcgtcggcgt gctcgagcga gcagtccgcg ccggggaacg cggcccggag
    59221 acgggcgagg acctcgggcg cgggcgcggg cgcaggcttc ggcttcgggc ggacgggcat
    59281 gccggcggag gataccgccg ggcggcaccc gccgcgcctt gattccggcg ggggcgggtg
    59341 gaagactccg ccgcgcgtga gcatccgacg cccgcttctc ctcctcctcc tcgccctcgt
    59401 cctcgccgtt cccgccctcg cggcggagcg ggtgaccctc acggtcctcc agacctcgga
    59461 cctccacgcg aacgtcctgc cctggaacta cgcgcgcgga gaggcggctg actggggcct
    59521 cgcgcgcgtc gcgacgcgcg tgcgcgagat ccggaagagc tcgaagcacg tcctcctcct
    59581 cgacggtggc gacacgatcc agggggcgcc ggccggctgg ctcgaggccc ggcgaccgaa
    59641 gggggagccg cacttcatgg cggccgccat gagcgccctc cggtacgacg ccatggccgt
    59701 ggggaaccac gagttcaact tcgggctcga ggtgctccgc cgcgcgcaga aggactcgag
    59761 cttcccgtgg ctctcggcga acacccggca cgccgcggga ggaggccccg ccttcgccga
    59821 gtacgtcgtg aagacgctcg gcggggtccg ggtcggggtc ctcgggctga cgacgccgaa
    59881 catccccggg tgggagccgg aggcgaaccg gcccggtctc gagtgggaga accccgtcga
    59941 gtcggcggcg cgcctcgtcc cgctcctgcg gcagagggag cggtgtgacg tcgtcgtcgt
    60001 cctgttccac agcggcctcg agatcgaccc cgcgaccgga gaggccaacg ggaccgacta
    60061 cgagaaccac gtcgtccgcc tcgcgaagac cgttccgggg atcgacctga tcctcaccgg
    60121 gcactcgcac cggcgcctcc cgctcacccg cgtccacggc gtccccgtga cgcagcccgg
    60181 ccgctggggc gaggtcctcg cgcgggtcga cctcgtcgtc gagcgcgcga aggggatcgc
    60241 gagagtcgtc gaggcccgcg gagagctcct cccgtcggac gggaccgtct ccgtcgaccc
    60301 cgagatcgct gcgatcgcgc gcccgcatca cgagcgggcg ctggcctacc tcgacgagcc
    60361 gatcgcgacc gccgccggcc ccttcccggc cgaccgggcc cgcctcgagg actccgcgct
    60421 cctcgacctg atcaacgaga cccagctcgc cgcgacgggc gcgcagctct cgatgacgtc
    60481 gctcctcccg taccggcacg acggctggaa ggaaggcccg gtcaccgtcc gcgacgtcta
    60541 ctccatctat ccgttcgaga accagctcgt cgtcgtggaa gtcgacggga agcgcctccg
    60601 ggcgctcctc gagcacgccg cctcgttcta cgaatcggcg gagtggaagg acggccgcct
    60661 cctgctgacg ccccgcgccg ggatgcggcc gtacaacttc gacgtggtcc agggggtgag
    60721 ctacaggatc gaccccacgg ctcccgccgg aaggcgggtc cgtgaggtgc ggtatcgagg
    60781 ccgcgaggtc cgagacgagg acgtcttcac cctcgcggtg aacagctatc gcgcgcaggg
    60841 cgcgggaggg tacgcggcgc tccggggcac ccgcgtcgtc cgggcctaca acgacgagat
    60901 ccgagacctc ctcgtcgagc ggctccggag cgccgggacg atccacccgg tgaccgaccg
    60961 gaactgggtc ctcgctccgg agacgtcgtg ggcgccgccg ccggcggcgg cggacgcacc
    61021 ggcgccgcgc tagcgcgccg gaaggagcca cgggaagggc cccctcgcct cgcgggcgag
    61081 ggggcccggg gggcctttcc tcgagggccg gccgcgcggc gtctcagctc gccgggacga
    61141 cgtacgggac gacggcgtcc gcgtccccgg tcttctggtc ccgccgaacg aggagagcga
    61201 agacgggctg cgacccggcg accggagaga cgtcgatccg gccgatcggc gtgccgaagc
    61261 cggcgagcga atagaacgcc gcggggaccc tcaccgattc cttcgggccg agcgggagcg
    61321 tgaccggctc gcccgccgcg accccttccg tgtcgaatgc ccggagcgtc acttcgccgg
    61381 ccgtgtcggt ggggttcgtg agctggagct ccgactccat gacgtcccac ccggcgtcgg
    61441 ccgcctggat gaagacggcg cgcgagccgg ccgcggcggt cgagtcggcg cggagggcgg
    61501 gcatgccgta gccgtggcgg ccgccggacg cgtccgcggc cgtgacccgc gcgaacgcgt
    61561 ggacgggcgg atcggagacg acgtcgaggg acccggcgaa cacgtcgacg ccgaacgtct
    61621 cccggacgac gtcgtccagc gcgacgacct tccccgcggg gaccgtgagg gagacggccc
    61681 gcgccggagg cgcggccccg ggcgaaccgg cgaggcgggc cgcgcggagg acgagcgaga
    61741 cggaggcgtc gcccgccgcg ccgttgaaga gctgcaggtc ggtgcgcgac gtcggaccga
    61801 gcgggccgcc gcccctctcg acgccgaaca cccactggtt cgtggccgtg gccctcgggc
    61861 cgaagaaggg aaggtcgatc gggtcggacg acaccgcgtc gatcgaggtg ccgaacggca
    61921 ggacgcgtcc cttcgccacg gtgtagacga tcctggcgga cggggagggc ggaattccga
    61981 aggccgagaa gaccgtcgag gcctgaaggt ggtatccggc cggccagcgg aaggcgaacg
    62041 gggcggagag cggagtgccg gcggcgctca cgaccgagac cgtcccctcg gccgcctcga
    62101 cgacgaggag gcccgcgttc acgcgggagg ctcctccgtc aggcggcgtg aagaggaatc
    62161 cggtctcgcc ggtcgcgagg gcctcggccc ccttcacgta gttccgcgcg aagccgtacg
    62221 tcccgccgtc gtcgcgcgcc cggtagacgc ggatccacga gtggacgtcg ccgaaccgcg
    62281 tcgtcgtcgc gcccgacgtg agccggagcg cgccggaggt gtccggcccc ttcatctcct
    62341 ccccgatcgg gtcggcctgg gagacgatct tccccgggcc gatcgtcagg ttcgccgtgg
    62401 gggagagacc gatgatccag tccgtcggca ggagtcgctc gttcacggtc gcgtcgaccc
    62461 ggaccccggc cgtgtcggcg ctcccgcgat tccaggtcac gacctgcgtg gcccagcgca
    62521 cgccgccgcg gccgccgatc gaagaggcgg ccgggaagaa gtggtcgtcc aaggacgcgg
    62581 ggatgggggg gaggacctcg gcgatctcgg ccgtggcgcc gacgaggaac cgcttgcgcg
    62641 tctcgtcgag gaccagggcg tacgggactc cggagacggc ggtgtagcgg gcgagcccgg
    62701 ggaggcccga tcccgtcacc gcggccgcgt cggcgggggc gacgtccagc tcgaccgtct
    62761 cgaggaccgt cttcgtcggc gccaccgtcg cggtcgtctg ggtgaggacg atctccttct
    62821 cctcgagcgt gtcggtcgcc gcgggtgtcg acccgaacgg gtcgaagacg ccgacctccc
    62881 cgatcagctg ttcggtgtag acgatccggt ccttgtagac cttcatgtcg aacggctggg
    62941 agaagacgcc gattgcgtat cgcttcagct cccgggtcgc cgggtcgaac cgggcgagct
    63001 gcgccgtgcc gtagaaggag aaccagaggt ggccgtcggg gccgcggatg atcttcgacg
    63061 gctgggcggt cgcgtccgga agcgcccagc gggtcacctt cgacccgtcc ggcgcgaagc
    63121 gcaggagggc gccgcggagg aagtcggcgg accagagcga gccgtcggca tcgaggaacg
    63181 ggtaggtgag gctcgagatc gggttcgcgg cgtcggtcga gcgccagtag acgaacgtcc
    63241 ccgtggcggg gtcgaagttc gcgagcgtcc cgttcgtctc ggggagccag agcgtcccgt
    63301 cgcccttccg gacgaacgcc gtcggccggc gggccccgtc ggcccactcc gtcagctcgg
    63361 ccgtctgcgg gtcgaacagg gagacctgga agccgccgag ctgcgcgacc cagagcttcc
    63421 cgtccggctc cttcaggaga tggctcggcg ccgcgtcgcg cggcatggtc cacttccgga
    63481 ccttcttctc gtcggccgag atccgcgcca ggacgttctc gttcgccgag gcggtccaga
    63541 tggtgccgtc ggcctcgagg atcatgttcg aggcgtcgta ccggccgccg ttcggtatcc
    63601 cgaggctggc gaccgacgct tcgatcgtcg tcgaggggat cggcgtcggg gtgggcgtcg
    63661 ccgtgggagt cggcgtcggt gtgggcgtcg gcgtctgggc gaagagggga agggccgtcg
    63721 ccgcgacgcc agcggcgagc gcggccatcc ggaagagtcg tgtcacgcgg cgcattctaa
    63781 cggctcggat gcaggaaccg ttccggcgct gtcggcgcgc ctagaatggg agggcgatgg
    63841 cgccagccga ctcggtcttc gagcgcctcg ccggcctgac ggcgaaggcg acgctcgtcc
    63901 gggaggagac cgggaagctt cgctgtctcg cctgcggtca ccgctgcctc ctcgccgagg
    63961 gggggcaggg ggtctgccgg gtccgattcg tccgcgacgg cgagctccgc gcgccgtggg
    64021 gatacgtggc ctcggtcgcg gccgacccaa tcgagaagaa gccgttcttc cacgtcctcc
    64081 ccggctcgac ggcgctttcc ttcgggatgc tcggctgcga cctgcgctgc ccgtactgcc
    64141 agaactggga gatcagccag acgcttcgcg acccgaatgc ggatgattgg gcgcacccga
    64201 gcccggccac cccggagggg atcgtcgccg ccgcgctcga ggcgggagcg caggtcgtca
    64261 cgtcgaccta caacgagccg ctgatcaccg ccgagtgggc ggcggccgtc ttcgccgagg
    64321 cccgccgggc gggcctcctg acgagcttcg tctcgaacgg gcacgcgacg cccgaggtgc
    64381 tggacttcct ccggcccctc gtcgacgcct acaaggtcga cctgaaagga atgcgggagg
    64441 ccgcctaccg ggaggtcggc gggcggctgt cggtcgtcct cgacgcgctg gcggggctcg
    64501 tggcgcgagg cttctgggtg gaggtggtca cgctcctcgt gccggggaga aacgactcgg
    64561 acgaggagct ccgcgacgcg gcccggttcc tcgcgacgct ctctcccgat ctgccctggc
    64621 acgtcaccgc gttccatccc gactacaggt tgggcgacgt ccgggccacg cctgccgcga
    64681 cgctccttcg cgccgtcgac atcggacgcg gggagggcct gcggcacgtc tacgcgggga
    64741 acctgccggg gggcgtcggg gacgccgaga cgacggtctg ccccggctgc tccgagcggc
    64801 tcgtcgagcg gcggggattc cgggtcgtct cgcgccggat cgacgccgaa ggaaggtgcc
    64861 cgcgatgcga gcggctcatc ccgggaatct ggtcgcccgg aggacgggtc gttctgcggg
    64921 cccccggcgg ccggccggcc gaaggtgaag acgtcccgac gggctgacgg tgccgaattg
    64981 acggacggtt cccttccggc atagcattcc cgggtctggc ccggcgcggc gcggccctgc
    65041 ggaagcgggt ggcgtgaacg gggcgccggg cgagggagtc gcgaggatca cgacccgccc
    65101 tccccccttc ggaccgagta ggaaatgccg ttcgcccgag ctgaacgctc gcgtgggcgg
    65161 cgcacaggaa acggaacggc ctcgatcagc ccgttccgga ggtcagcttg gacgaattcg
    65221 acccgaaaga gcccgtagaa gcggatcggc cttccgtcga gaccacggtc gtcgaggagc
    65281 tcccggaggg agacgacgac gccgaggccc tcgacgaggg cgacggcgag acggaggccg
    65341 tcgcctcgga gggaggcagc gttcccggcg agccatcgcg gaaacggcgc cggcgccgcc
    65401 gccgccgcgg ccgcagcaag gccgccggct cggaaggggc gcccggagcc gaatcgtcgg
    65461 gcgaaggggc gccgcggcgg gagctcgctc cccaggcggc tcctccggtc accgtccgag
    65521 gcctcgtcaa gctccatgga gaccactccg gcctcgtcgt ctcgcccgag acgttcttcg
    65581 cggagaaggg ggaccccttc ctgccgaagt acctcgtcga gggcgaggat ctcgaggacg
    65641 gcctcctgat cgaggccgaa gcccttgtcc gcggtccgaa ggggccgatg gtccagaaga
    65701 tcctctcgat cgagggaatg acgccggccg agtaccgttc gaagtacgtc cagttccaca
    65761 aggggatctc gatcgacccg aaccggcgcc tccggatgga gacctccgcc gacgagaact
    65821 cgggacgcgt cctcgacctc gtcgtcccgc tcggccgcgg ccagcgctgc ctgatcgtgg
    65881 cgccgccgaa ggcgggaaag acgttcctcc tgaagacgat ggcgaacgcc atcgcgaaga
    65941 acgaccccga cgtgatcatc ttcatgctgc tcgtcgacga gcggccggag gaggtcaccg
    66001 acatgcagcg cggcatcacc ggacaggtca tcgcctcgtc cgccgacatg aacgccgcgc
    66061 accacatcgc cgtcgccgag gcgacgttcg agcgggccaa gcgcctcgtc gagatcggga
    66121 aggacgtcgt catcttctgc gactcgctga cccggatgag ccgcgcctac aacaacgagc
    66181 agcgcgggag cggcaagatc ctctccggcg gcatcgacgc gcggacgatg gagaagccgc
    66241 ggcgcttctt cggcggcgcg cggaaccacg aggacggcgg ctcgctgacg atcatcgcga
    66301 cctgcctcgt cgacaccggc agccggatgg acgacgtcat cttcgaggag ttcaagggga
    66361 cggggaactg cgagatcatc ctcgaccgcg gcctcttcga ccggcgcgtc ttcccctgca
    66421 tcaacatccc ggcctcgggc acgcggaagg aagagaagct ctacacggcc gaggagctgc
    66481 cgaagatcca caagctccgc cgcgccctct cgtcggtcaa gccgatggac gcgatggagc
    66541 tcctcctctc gaagctgacc cggacgaaga cgaacgccga gttcctgaag tcgatcgcct
    66601 gaccgtttcc cggggcacgg gcacgaaatc gggggccgcg agccccccga ctcgctttcg
    66661 cggccgtccc ctcccacccc tctcccgtct tcatcagggc gcgcgcgccg gcggcgcgcg
    66721 cgcatgaggg cggtgggtgg ggggagaggg gtgggttcta gggctcgatc tcgggctcgg
    66781 ggaggaagaa gtcggtcagc ctgcgcatga tcgcgcggct ctcctcgacg tggaggaagc
    66841 gggaggtgtc ggggaagacg aggagctccg cgcctgggat tcccgcggcg atcgtccgcg
    66901 cgcagcggat ccggtcctcc tcgtcgcgct cgccgacgag aacgaacgtc cgcgccgaga
    66961 ccgcgccgag gcgcccgacg agcgcggcgc cggcgcgagg aaggcgctcg aagcggcccg
    67021 ggctctgcgg gaagcggttg aagatgtcgg cgatcctctc gaagatcccc tcccgctcgc
    67081 gggccgcgtc gaagatcggg gaggggagga gcttctccgc gagcgccttc ggagcgtcgc
    67141 tccggagagc ggcccgaagg tccgccgcgg cggccgccgc ctcgggagag gaggagaagc
    67201 cctcgagcga cggcgccagg agcgcgatcg acccggtgcg gccgggattg cggagggcga
    67261 actcgagggc gacctcggcc ccctcgtcgt gcgcgaggaa gtgggccttc tcgacggaga
    67321 accagtcgag gatcccgagg acgtcatcgg cctcctcggg gagcggcacg ggaccctcgg
    67381 tcggcggcgt cgtccggccg cacccgcgtc gatcgaagcg caacgcgcga aaccctgacg
    67441 ccaggcccgt cgcgggggcc tccatcaggt cggcgttcgc gagggcgccg tggaggagga
    67501 tgacgagatc tccctcgcct ccgacctcgt agtagaaacg ctggtcccga actccggcaa
    67561 acggcatggc gttcctcccg gaggaggaat cataagccgc cggagacgcc gggggtcagg
    67621 aggcggatgg agcggacgcg ggcttgcgcc ggcggtggcg cttccgccgc gaggcgccgc
    67681 cgggcgccgc cggggaggcc gagaccccga accccccttc ggggatctcc agctcgaacg
    67741 ccgcgcaggt ccagaagtcg gtcggcgcga cgtcgcccgg aggcggctcg acggccccgg
    67801 ggaggtcggc tgcgggctcg gcaggggcgc cggacttcgc ggcctcgacc ctcgcgacgg
    67861 cgagcggagc ggccgcctcg gcgaggacga ctgcctcggc gcgcacgacg ggtgcgaccg
    67921 gcgcgacagg gatgtcgggc acgaagggct cgggggccgt ggccggcgga gcctgcggct
    67981 tctcgacgcg cggggccggc gcggcgggca cgggctcggg gcgcgggggc gcggcggccc
    68041 gctcccgccg cgggcgcgag gccctcgggg ggggcggggc tttcgtgtcc tcctcctcgt
    68101 cccccgcgac gggggcggcg gagcgcggcc ctcccgagac gcgcaccggc tcgcgaagcg
    68161 gtgcggggcg acggacgaag agcgcgtcgt cgggatgctc gaccgggatc ttcatcccga
    68221 tcagcttctc gatggaggag agcgactgga cgtagttctc gcaggccagg gagatggccc
    68281 ggcccgagtg gccggcgcgc gcggtccggc cgatccggtg gacgtagtcc tccgggtcca
    68341 tcggcaggtc gacgttgatg acgtgcgtga cgccgtcgat gtggagcccg cgggaggcca
    68401 cgtccgtcgc gacgaggatc gggagcttcc ccgacttgaa gtcggcgagg atcttcagcc
    68461 gcttcgcctg ggggatgtcg cccgagatgg cgccgcagcg gaggccgtgc ctctcgaggc
    68521 gctccacgag ccgctcggcg aagcgcttcg tgttcacgaa gagcatcgtc cgggtcgcgt
    68581 tcccctcacg ctgcaggagg ccgacgagga gcgagagctt ctcggagttc ccgacgtgga
    68641 agaggctctg gacgacgcgg tcggccgtca cgtgctccgg agcgatctcg acccggacgg
    68701 cctcgttcat gtgctcccag gcgagctccg tcacccgcca ggagagcgtc gccgagaaca
    68761 tcatcgactg gcgccggcgc gggggggggc accgccggag gatccgccgg aggtcgggga
    68821 ggaagcccat gtcgaagaga cggtcgcact cgtcgatgac gagcgactcg accgacccga
    68881 acgaggtcgc cccctgcccc tcgtagtcga ggagccggcc gggagtgccg atcaggatgt
    68941 cgcagccggc ctggaggagg ctccgctgcc tcacgtagtc gaggccgccg aagacggtca
    69001 cgatgcgggg cctcacgaac tgcgaaagga ggatcgcgtc gtgctcgatc tggacggcga
    69061 gctcgcgcgt cggagcgacg atcagggccc ggggcgctcc cggcttccgg ttcgcggagg
    69121 agggcgcctc gacgaggcgg gtgaggatcg agacgaggta cgccgccgtc ttgccggtcc
    69181 cggtctgcgc ctgcgcggcc acgtccttgc cggcgaggag gagcggcagg gtgctggcct
    69241 ggaccggcgt cgcccggacg aaaccacagg cggcgatccc ggcccggacc gcgtgcggga
    69301 ggggaaggtc ggtgaagaag acgtcgctga ggtagtcgcc ggtgcccggc ggagaggagg
    69361 acatccgggg ggattgtagc cggacccggg aaggccgcgc cggagggacg ggaggcgcgc
    69421 ccggcagtag catggggcga tgagcgccgg cgcctccctc cggatcgtct cgccctcgac
    69481 gggcgagctc ctcgcggacc ttcccgcggc gggccgggcc gaggtcgccg cggccgtcga
    69541 cgcggcgcgc gaggcggccc ccgcctgggc cgcgaccccg cttccggagc gggtcgccgc
    69601 gctgaagcgc tggcgacggg ccgtcgtcga cgacgaggag ctcctccgga cgctcgtccg
    69661 cgagagcgga aagccgcgac acgaggccga aggggtcgag ctcctctact tctgcgagct
    69721 gacccggttc gtcgccgccg cggcccggcg ggcgctcgct ccggagaggc gcggaacgct
    69781 cctcttcgcc gtccggagga ccgacgtcca ccgcgtcccg gtcggcgtcg tcggcgtgat
    69841 cggtccctgg aacttcccga tcctgaacaa cgccgccgac gccctcgcgc ccctcgccgc
    69901 cgggaacacg gtcgtcctga agccgtccga ggtgacgccg ctgacctctc tccggatgga
    69961 ggagctctgg cggaaggcgg ggaatccgcc cggcgtcttc cgtgtcgtcg cggggcgcgg
    70021 cgacgccggg gcggcgctcg tcgacctcgt cgacggtgtc atgttcacgg gagccgtcga
    70081 gaccgggcgg agggtggcgg cgcgctgcgg ggagcggctc gtcccgtgcg tcacggagct
    70141 gggcgggaag tccccgttcg tcgtcctttc gggcgcggac ctcgaccggg cggcgcagtc
    70201 ggccgcctgg tcgggtttct tccacagcgg ccaggcctgc atccggtcgg agcggatcct
    70261 cgtcgaggag cgcgtcgcgg acgagttcga gaggctcttc ctcgaaaggg tgagggcgct
    70321 gcgacaggag gtgcccgccc cggacgccgc gcgcgagcac gacctggggg ccgtcacgtt
    70381 cccccgccag atcgaggtcg tgacgcggca gctcgaggat gccgtctcga agggtgcccg
    70441 gctcctcgcg ggcggccgcc cgcgagccga cctcggtgga gcgttcttcg agccgacggt
    70501 cctcgcgggc gtgacggcgg agatggcggt cttccgggag gagacgttcg gcccgctcgt
    70561 cccgatcgtc cgcgtcgccg acgcggacga ggcgctccgc ctcgcgaacg actcccacct
    70621 cggcctgagc gccagcgtct gggggccgcc ccgcgcggcc cggcgcctgg cggagcggat
    70681 cgaggcgggg agcgtcgccg tgaacggcgc gctcgtccac tacctcgtcg tctccgcccc
    70741 gctgggcggc gcgaaggcga gcggcgtcgg cgtccggcac ggcgtcgagg ggatacgtgc
    70801 cttcacccgc tcgcgcgccg tgagcgtccc gagggccctc ctcgcgccgc tcgaccggct
    70861 ccttctctcc cgcctggcgt ttccctacga cagccgcgtc ctcggcctcc tccggcgtgc
    70921 ggtccggctc ctgtaccgga ggggatgagg agccgggggc gccccggtcc cacgcgtcta
    70981 caatccgccg ccatgaaggc gacttctcgc gccgtgccgg cgctgctcct cgtaatgttc
    71041 gcggcccccc tcctcgcgca ggcgccgaag ccggcgccga ccgcccctgc cggaggggcc
    71101 atcgtcggga cgaacccgca ggggcctccc ggctcccggc cggccgagat ccaggacgtc
    71161 cgggtgctcg gggcctcgcc gagagacccc tggcagatgg agcggctcgg cgtcgccgcc
    71221 gtccagaaga acgagctcgg gaaggcgagg gagttcttcg aggagtcgtg gaaggcggga
    71281 gagctcccga ccgcgcccta caacctcgcc tgcatcgacg cgcgcgaggg gaaggccgac
    71341 gccgcattcc gacagctcga ccgcgcgatc gccgccggat tcgacgacga ggcgacgctc
    71401 gcgaaggaca ccgacctcgc gtcgctccgg gggcgccccg agttcgcccg gatcgtccag
    71461 ggggcgaggc ggaatcgcct ggccggagac gaggcggtcg tgaaggaggg ggtcttcgtc
    71521 tctcccggga ggccgcccaa gggaatcctc ctcctcctgc acgaggtcaa ctccgacccg
    71581 atgtcgatcg cgggcccctt cgtcgcggag gcgaaggcgc gggacctctt cgtcgccgcg
    71641 ccgcgagggc cggcgcggtc cggccggaag cgtttcggct ggggctcgcc cgaccgcgcg
    71701 ctcgaggcgc tgaagcggac gatcgccgag gcgcagacgc gggcgggcgg cctcccgatc
    71761 ccggtcgccg tcgtcggcgc gggccgtggc gggaagctcg ggctggagat tgccgcgcgg
    71821 aaccccggca tcttctccgc ggtggcctcg atcggaggga tcttcgaccc gggtccgacg
    71881 ggggccgctg gcgtcgccgg cctgcggggc gtgccggtct tcctcggcgt cgcgaagagc
    71941 gcccctcccg agctcctgcg ggcgatgcag cggggtcggg agaacatgcg gaagctcggc
    72001 gtgaaggtgc ggtggggcga gtggccgggg acgggcgaag gccttccggc ggacgcggcg
    72061 gcggcggcga aggacgtcct cgacgccatc cgctcgcccc gccccggggc cgcaggctcg
    72121 tcggcgaagt agcgggcgtc ggcgcggctc agagagccgg gccgacggcc tccacgatct
    72181 cgaggaggtc gccgtcgggg tcgagcgcga cccccacggc ggccgccccg ccgtccagcg
    72241 ggacgaagaa cgtctcgtcg gcgcgtgtca cctcgacggt cgccgccttc gagccgccgt
    72301 tcgccgtgaa gacgcggagg gtgagcggga agacgtagtg cggcacgtcg ccgggacccg
    72361 gccgggggtg gacgacggag tgcgtctgca cctgcctcac gttcacctca gcgccgccgg
    72421 aaacgttccg gaaggtcgct cgcaggatcg gccggtaggg cgtctcgacc cactggtcga
    72481 agtgctgctt caggtcgcgc cccgagacct cctcgaatac ggcccgcagg tcaccccgag
    72541 tcgcgttccc cctctcgtgc cggcgccggt actcgcggac ggcggcgaag aacgcgtcgt
    72601 cgccgatccg ccttcggagc atgtggagga cccacgcccc cttggaatag acggctcccg
    72661 tgtggcggaa cgggtccgac gggggggcga cgaccgcggg gccgagctgg ccggagtaga
    72721 gcccgtcgtc gtaccgggag gcgaggagca ggccgggaga gaagctcgag gtccgctccc
    72781 ggtagaggac ctccgcgtat gtcgcgaagc cctcgttcag ccagatgtcg tcccaggtcc
    72841 gcatcgtcac ctcgtcgccc caccactggt gagccagctc gtggacgagg aggtccgtca
    72901 ggtcgcggga ggcgttcccg aggagcgagg cgccgatgag agtgatcgtc gggtgctcca
    72961 tcccgccgga gaagctcccc tcgacgatcc cgtacttcgt gtcgagatac gggtagtccc
    73021 cgaacaggga agagaggacc tccaggtggc ggaccatcga ggcgacgcgg gcccggttcg
    73081 gagcggcgtg ctcggggacg acccatccga cgaccggcat cgtccgcccg tcggccgtcc
    73141 ggtattccac ggcgatctcc tcgaatcggc cgaccgccac cgacacgagg taggtcgaga
    73201 tcggctgcgg gagcctccag cgccaggtca cccgcccgtc cggctcgctc gtcgaggcgc
    73261 cgcggccggc ggagacggcg acgtacccgg aagggaccgt cgcggagagc gtgacgaccg
    73321 ccttgtcggc cgggtcgtcg acgcagggcc agaaggtccg cgcgtcgaaa ggctcggcga
    73381 gggaggagat cgccggtccg ctcgcggggg cggtccagaa cgtcagggcc ccgcccgcga
    73441 gcggggtccc gccccagcgg acgacgaggg tcgtccgctc ttcggccccg agcggcgggg
    73501 tgagcgggac cgtgaggacg tcccccgccg cggtgaaggt cgtcacgggc gagccgtcgc
    73561 gctcgacgcc gcggacgctc atcgcgtcgt ccaggtcgag ccggacctcg gagacgccgg
    73621 gccggatggc ggcgagccgg atcacggcgc ttccttcgag ggcgacgacg gcggggtcga
    73681 gctcgaagcg gaggtcgtag gtgaggacgt cgtagcccct cgccgcgcct tccggagaga
    73741 gcgcgccccg tccggaccgg ctccgggcga gctccgagac gagggcgcgg gcgcgcttcg
    73801 cccggccttc ggggtgtccg gcgcgggcga ggcgctcgtg cccctcgtgg tcggaccagg
    73861 cgttcacggc ggcgccgccc gagagaagcg cgacgaggag cgagaccggg agagaacgac
    73921 gcatcggcct gatcctcaga gcagcggggc gagctcctcg gcgcgggcga tcctctcgcc
    73981 gagcccgagt ccgtcacgcc aggttccggt gacgacgagg cgcggccgct cgcgtccggc
    74041 gttcaggtcg tcgaggtccg cgtccagccg tcgaagggtc tcgtggtggc cgacgggcag
    74101 acgcggaagc gccgacggcc acttcagcgc ccttccgtgg agccgtggtc cgaggtccgg
    74161 gtgcaggcgc cggacctcgg cctccgcgat ccggagcagg tcggtctcgc agaggtcgag
    74221 ggccgacggg tcaagcgccc ctccgaagaa cgacgtcgtc agcgcgaccc cggccggaac
    74281 gcgggaaggg aagagagagg agaggtagag caccccgagc gagcggagcc cctctccgcg
    74341 gggggcgagg aggccgaacc cgcggggggc cgcgtcgggg gaatcggccc ggaacgcctg
    74401 gaggacgacc gcgaggcgcg agtacggaag cgtcgcgaga cgggcgcccg agcgcgggag
    74461 ccgcggtccg agcgcctccg cgaagctggg cgcgtcgagg gtcgagacga gggtgtcggc
    74521 cgggtagacg gtcccgtctg cgatctggac cttccagacg ccgccgtttc cctctccgtc
    74581 gaatggtcct tcgatgccgg cgatctcggc gtccagctcg atcctcactc ccgcgcggac
    74641 cccctgctcg gcgagacgct cggcgagcgt tccgagcccg ccccgaaagc tgatcgtcct
    74701 ccgcgaccgg ggtctcggcg ctcccgcgag gccgcgcagg acgctcccgg cccggccttc
    74761 ggcgtcccag agggcgggga acgcgctccg gaccgaaagg gtccgcgggt cgtcggccca
    74821 gacccccgag acgagcggct cggcgcccca gcgggcgacg ccggggccga agcgcctctc
    74881 gaagaagtcg tggaccgatt cgtcggccag gtccgcgggg cgtcgggcga cgaacggctc
    74941 agccagggct cggagccggg ccgcgagcgg gagcgccccg ccgagaagga gcgacggccc
    75001 cggggtcacg cgacgcatcc tgccgccccg gacgatccag cgcggggacc tggggtcggc
    75061 caccaggacg tcgtcctcga ggccgagcgc ttcgacgaga cggtcggaag cgggcgtcgt
    75121 ccggagcgtg accggcccgg cctccaggac gagcccctct tcgcggatcg ttcggacggt
    75181 cccgcccggg ctcggtccct cctccaggat caggacgtcg tcgcccggag ccgggcaccg
    75241 gttgcggaga tagccgaggg cgagcccggt gacgcccccg ccgaggatga cgatccgcct
    75301 caacgagctc ccggcgcgag gagcgccttg cggcagacgt cggcgagcgc gtcgacgaac
    75361 gcggggtggt cgttcaggcc gtccgcggcg acgtagtgcg tgatgccggc cgccctcgcc
    75421 tcctcggcga agagcatccg gatctcgtag agcgtctcga cgtggtcgga gacgaaggcg
    75481 atcgggcaga cgacgacctg cttcaccccc tcgctcgcga gccgggcgag gacctcgccg
    75541 gtcgccggtc cgagccaggc gaccggaccg agcttgctct ggtagccgag gcgcgagcgg
    75601 aagcgcctgc cgaggcgctc gtcgacgagc gcgcagcact cctcgatctg cttcgggtac
    75661 gggtccttct tcttctctac cgtcgagacg gggagcgagt gggccgagta gaggaggacc
    75721 gtcgccgccg ggtcggggtc ggggagcgtg gccgccgcgg cgaggatcct ctccgcgtgc
    75781 gcgtccagga acctcggctc ggcgtaccag gagtccggcg cgtgcgcctt ctcgggcgac
    75841 caccccatcc gcgagagcgc ctggtcgacg cgcgcgaacg tgctccgcgt cgtcgtcacc
    75901 gagtactggg ggtagagcgg gaggacgaag aggcgcgtca cgcccgcggc gcggaggtcg
    75961 ggcaggacgt cctcgacgag cggggcgctg cacgtcatcg cggctcggac gagaggtggc
    76021 tcgccgggcc acgtcggcgc gagggccgcc tcgaggagca tcgccgacct ctccgtccag
    76081 cagcggatcg gcgaccagcc gatcttctcg tacgtcttcg ccgactcggc ttcgcgcgtc
    76141 gtcgcgatcg tccaggcgat cgccttccgg agcggcgcga aggggatgcc gaggatctcc
    76201 gggtcggcga agagtcggta gaggaagccc ttcagctcct cccgcttcgt cggaccgccg
    76261 agctggacga ggaggatccc ggtcttcccg gcggccatca gagcgtcctg gagaagacct
    76321 tcggctggtc ggccggacgc ctcaggaggt aggggtcgaa gagcatcgcg acgttccgga
    76381 tgaagatccg gccgagcggc gtgacgcgga cctcgtccgt ccggggctcg acgaggccgt
    76441 ccgcgacgaa ggcggggagc gccgcgacct cgcgcgcgaa cgtctcgtcg aaggaggcga
    76501 ggccgaactg ccgggcgatc tcgcgcttgt cgatgacgca gtggcagagg aggttcgtga
    76561 tgaccgtccg ccggaggtgg tcctcgctcg tcagccgcca ccccttgatc gtcgaggggc
    76621 gcccctcggc gcaggcggcg tggtaggcgg cgtagtcctt cgtgttctgg acgtagcagt
    76681 cgtcgatctg ggcgatcgcc gagacgccga gggcgtagag gtcgacgccc gcgtgcgtcg
    76741 tgtagccctg gaagttccgc cagagcgtcc gctccgcctg cgcgcgcgcg atctcgtcgt
    76801 gcggcagggc gaagtggtcc atcccgatgt agacgtagcc cgcctcggag aacttctcga
    76861 tcccggagac gaagatcgcg aacttctccg tcccttccgg caggtgcgcg gcgaggacgc
    76921 gctgcggctt cttcagccac gggacgtggg cgtaggagta catcgcgatc cggtcgggcc
    76981 ggagcgtcag cacctgctcg acgcttcgcg agaagccggc gagcttctgc cccgggaggc
    77041 cgtagatgag gtcgaggttc agcgactcgt agccgagctc gcgcgaggcg ccgacgagcg
    77101 ccttcgtcat ctcgaacggc tggacgcggt tcaccagctc ctggacctcg gggtcgaagt
    77161 cctggacgcc catcgagacc cggttccagc cgtggcgccg gaggaccgcc aggtgctccc
    77221 tcgtcgtgac gcgcgggtcg acctcgaccg agatctcggc gcccggcgcc ggacggaacg
    77281 ccgcgaggag gtgctccgcg agggcgtcgg cctgcttcgg cgtcaggtag gtcggggtgc
    77341 ctcctcccca gtgaacctgg acgacggggc gcgaccggtc gacgcgggca gcgaggaggt
    77401 cgatctcccg gtggaggtcg gcgaggtagc cctcgatctc cccccgctcc tctccctgac
    77461 ggtcgggctt cggcacgacc ttcatgaaac agccgcagaa ccagcactgc tcgtcgcaga
    77521 acgggaggtg gacgtagagc gagatcggcc gcccggccgc gttggcacgc tcgcaggcgg
    77581 cctcgaagtc ggcgggcccg atcgtgtccg tccactcggg cgccgtcggg taggaggtgt
    77641 agcggggtcc ctggacgttg tacttggaga gcagctccgt cgtcatcggg agcgtctcgc
    77701 cctggaggcg gatcgggatc gcgggcacga gcggagatta cacggacggg agggtacggt
    77761 cgcgggcggc gcgcgtcggg gacggaccgg tcggcctaga attccccatc ggcgacgggg
    77821 acgccgggaa aggcgcagga ccatgccgga agagagccgc cctccgttct cctgggtgaa
    77881 cgtcccgccc tccgagccgg tccggtcgga ggcggcccgc gggctcctcc gggccctttg
    77941 cgatcccggc cggctcgaga cggccttcca gccggtcttc cgggcccgga ccggagagct
    78001 cgaggggtgc gaggcgctgc tgcgcatccc cgccggctcc gggttctcgg gccccggcga
    78061 ggcgttcacc gttgcgctcg ccgaggggct cggaggagac ctcgaggccg cggcgatcca
    78121 acgagccctc cgggacgccg agccgttcgc cggggaccgc ctcgtcttcc tgaacctcct
    78181 cgcgacctcc ctctcggact cccgcctcgg cgcccgctgg ctcgtcgagc gggcggtggc
    78241 ccatggccgc gttccctccc agctcatcct ggagctcggc cagatcagcc ggatcgtcga
    78301 cttcgccgcg ctcgcccgcg cgctcgagcc gtatcgcgag gaggggttcc ggatcgcgct
    78361 cgacgacttc ggctccggct acacgaacct ccggatgatc accgacctcg cccccgactt
    78421 cgtgaagctc gaccgcgtct acgtcgagaa cgtcttcgtc cacgcccgaa agcggatcct
    78481 cgtcgagtcg gtcgtctcgc tctgtcaccg gatcaactgc ggcgtcatcg ccgaggggat
    78541 cgagacgccg gacgacctcg agacgtgcct cggcgccggg gtcgacctcc tgcaggggtt
    78601 cctcctcgcc cggccggggg cgccggaaga ggcgttcctg gcggaggacc tgacggtcga
    78661 agccgcgggc ggccacggcc ggggcgagat cgccgccgcc gtctcccacg ggccgacgct
    78721 ggcggtcgag gcgcccctgg cagccgcgga cgacctcttc cgggccgagt ccgatctcga
    78781 cgtcgtcccg gtcctccagc gcggccgggc ggtcggtctc ctggcgcgga cccgcgcgga
    78841 gacgttccgg ttcgtctcgc ccggctccgg ggccgacacc gtcgccgccg cgatcgccga
    78901 cgcgccctgg gaccagatcg aggagggaac gcccgtcgag gaggtcgcgg cgttcgtcgc
    78961 gcggaggccg cgtgcgcggc gcttcgagcc gatcgtcgtc gtcgggccgg ggcgtgtcta
    79021 ccgcggcctc ctgcgcctcg acgatctcct cggtctcttc gcgaggctct acgtcgagaa
    79081 cgggctggag tcgcatccgc tcaccgacct ccccggccgg ggccggctcg aggcggaggt
    79141 ggaccggcgc ctgacgcggg gagcgtcctt cgcgctcgcg cggctcaaca tcctccggct
    79201 gcgggcgttc aacgaccggt acggcatccg gcgtggcgac cggctcctcg cgcacgtcgc
    79261 ggccgtcctc cgaaccctcg cgaccgagga gccgggctcg ttcctcgccc actacggagc
    79321 cgacgacttc ggcttcctgc tgtcgcccga gagggtcgtc gagacgctgc gccgggccgt
    79381 ccaggggatc gcctcgacgg tgggggagtt ctacgacccg gaggatcttt cggccggcgg
    79441 gatcgccggg cacgatgcgc gcgggggggt gatcctcgtg gcccccgccg cgctcgtcgc
    79501 cggggtcgcc tcctggggcg gcggcggggc accgacgctc cggacggtcg tcaccgcggc
    79561 ggaggcggca ctccgggaag cgcgccgcgg agggacgcag ccagtcgtcc ggacggtcgg
    79621 cccggccgcg gcgcccgcgg cgactccgga gcggtcggag cagccgccgc ggacgcaggc
    79681 gagccttccg aagccgttcc gctgaagggg cgtcaggccc gggattcgcg ggcggcttcg
    79741 acgaaggcgg cgacgttctc gggcggcgtc ggcgggagga tgccgtggcc gaggttcacg
    79801 acgtgcccgc cctcgcccgc cgcggccctc gcggtgcgga ccgcggcctt cacgtcctcg
    79861 ggcgagccga ggaggacgcc cgggtcgacg ttcccctgga gcgccttgcc gcaggccctt
    79921 cgccgcgcct cgtcgagcgg gagcttccag tcgacggaga tcacgtccga ggccatctcc
    79981 gcgtacgagt cgatgaggtg cgccgagccg gaggcgaagt ggatgacggg gacctcaccc
    80041 ctcctcggga ggcgggcgag gagccgctcc gtcgcgggcc gcgcgaagcg gtcgtagtcg
    80101 gccttcgaca gctcgccgga ccaggagtcg aagacctgga cgacgtcggc gccggcctcg
    80161 acctgcgcgg cgaggtagtc gccgaccgcg tccgcgagct tcccgaggag cgcctcgagg
    80221 agcttcggct cgcgccacat catcgtcttc gtgtgctgga aggacttgct tccgcccccc
    80281 tcgaccgcgt acgtcgcgag ggtccagggc gcgccgcaga agccgatgac ggccgcgcgc
    80341 cccgcgacct ccttcttcag ctcgcggagg atcgccggga cgaacccggt cccgacggtc
    80401 gggtcgaacc cgcggagcgc ctcgacctgc gccgccgtcc ggaccggcgt cgggagcttc
    80461 ggcccccctt cgtcgagctt cacctcgatc cccatcggcg gaagggggat caggatgtcg
    80521 gagaagagga tgacgccgtc catcccgaag cgccggaagg gctgcatcga gacctcgacg
    80581 gccgacggca cgtcggcgca gaggtcgagg aacgcgaggc gcttgcgcac ctcgcggtac
    80641 tccggaaggt agcggccagc ctggcgcatg agccagacgg ggggcacgtc cacggggcgg
    80701 ccgaaggcgg cgtcgacgaa gcgctggcgg gaggggccgg gaaccgacat ggcgcgggag
    80761 gttagcatcg gcccctcgat ggctacgccg atcccccaga acaccgcccg attcaccccc
    80821 gccgaggtcg cccgcgccac cggcggcgtc ctcgtccgcg acggtcggcc cctcgccggg
    80881 gtctcgacgg acacccggac gctcgcccct ggcgccctct acgtcgcgct tcgcggcgag
    80941 acgttcgacg ggcaccgctt cctgccccag gcgaccgccg ccggcgcggg cggcgtcctg
    81001 atctcgacgg ccggcgacct gccgccgaac ggcgccgtca tccgcgttcc ggacacgcgg
    81061 gtcgccctcg gcgacctcgc ccggttccac cgcgaccgct gggcctcgac cgacgggagg
    81121 aaggtcctcg cggtcggcgg ctcggccggc aagacgacga cgacgcgcgc ggtcgccgcg
    81181 ctcctcgacg tcctccgccc cggcgaggtc cagacgacac ccggaaacct gaacaacgac
    81241 gtcggcctcc cgatgacgct cctcctcctc gacgagcacc accacctcgc cgccgtcgag
    81301 gtcgggacga gccaccgcgg ggagatcgcg cggctggccc ggatcgcgcg gcccgacgcg
    81361 gcgctcatca cgctgatcgc cccggagcac acggagggga tcgggacgct cgaggacgtc
    81421 gcggacgagg agggggacct cttcgccgcg ctcggggccg acggcttcgc gatcggcaac
    81481 ggcgacgacc cgcgcgtcgc ggcgcagatc gcccgcgctc ccggggaacg ccggatcctc
    81541 tacggcttcg gggagagctg cgacgtccga gccctcgcgc gcgaggtccg ggggctcgac
    81601 ggcagcgtcc tccaggtcgc ccgggagggg gcggaggcgg tcgcgtacga ggtccccctc
    81661 gccggggagg cgggggccta cgcggcgctc gcggcgctcg ccgccgtgcg cggcctcctc
    81721 ggagtcgacg cgccccccga ggcgctccgc gaggcgctcc tccgggtcgc gtccgtgaag
    81781 gacggccgat tcgcgccgga gacgctcgcc gacgggaccg tcctcctcga cgacacttac
    81841 aactcgaaca ccggctcggc gctctcctcg ctgaagacgg cgcgcgagct cgccgacgcg
    81901 ctcggacgaa ggctcgtcct cgtcctcggc gagatgcgcg agctcgggga gctcgccccc
    81961 gccgagcacg acgtggtcgc ggcggccgcc gtggaagccc ggcccgcggc gctcgtcgcc
    82021 gtgaacggcg aggcgcagcg gttcgcccgt gcggccgagg ccgcggggat ccccgcggcc
    82081 ttcttcgcga ccggcgaggc ggcggcgcct cgcgtcgtcg aggcggtccg tcctggagac
    82141 gtggtcctcg tgaaggcctc gcggggagtc cgcctcgaga ccgtggccgc cgccctccgg
    82201 gtcgcgcgct aagctgggga cggaggccga agagatgtcc acgccgctcg tcgggatcgt
    82261 catggggagc gcctcggacc acgagacgat ggcgaaggcg gaggaggtcc tccgcgactt
    82321 cggcgtgccg tacgagacct cggtcgtctc cgcgcaccgg atgcccgagg agatgttcga
    82381 ctacgcgaag ggagcggcgg cgcggggttt gaaggtgatc attgcgggag cggggggggc
    82441 ggcgcacctc cccggcatgg tctcggcgct gacgacgctc cccgtcctcg gcgtcccggt
    82501 ccggagccgg gcactctcgg gcctcgacag cctcctctcg atcgtccaga tgccgggggg
    82561 cgtcccgacc gcgacgttcg cgatcggctc ggcgggagcg acgaacgccg ggctcttcgc
    82621 ggtgaagatc ctggcgctct cggacccgca gctcgcagcg aagctcgaga ctcaccggga
    82681 gctgctccgg gagaaggccc gggcggggaa cgaggaggtc cggcgggtac gaggcgggac
    82741 cgtcccgtga caacggtcgg cgtcctcggc ggcgggcagc tcggtgcgat gctcgcctcg
    82801 tcgctccagg acctcggcgc ggacgtccac gtcttcgacc ccgacccgaa cgcgccggcg
    82861 ctccggcgcg ccgcgcgggc cgtgaccgca ccgtggagcg actcggcccg ccttcaggac
    82921 ctcttcgacg cctgcgacgt cgtgacgtac gagttcgaga acgtcgagac cgaggggctc
    82981 gcccgcgtct cgggcctcgc gaagctcgcc ccgtcgctcg acgtcctccg gacgacgcag
    83041 gaccgggcgt tcgagaagga gttcttccgc tcccacggtc tgccgcacgt cgcctttgcc
    83101 gccgggcggg acgcggacga cctgcgcgcg gcggcacggt cgttcgggct ccctgcgatt
    83161 ctgaagactg cccgcggcgg ctacgacggg aaggggcagt ggaagctcgc gtcggaggcc
    83221 gagctcgagg cgctcctcgc atcgcccgct cgcgccgccc tcgagtccgg cggatgggtt
    83281 ctcgaagagc cgatcgagat cgccctggaa gcgagcgcca tcgtcgcgcg cgagcggaac
    83341 ggggacgagg tcgtctttcc tctcttcgag aacgtccatc gcgaccacgt cctcgacgtg
    83401 acgctcgttc cggcgcgcct cccggaggac ctcgaggccg aggcgaagtc gatcgctctg
    83461 gcggcggcgc gggcgctcgg ggtcaccggc ctcctgacga ccgagctctt cgtcgggcgg
    83521 tcgacgcgcc accgtccggc agaggtccgt gtcttcacga acgagctcgc gccccgcccc
    83581 cacaactcgg ggcacgtcac gcgcaaggct tgtacctaca gtcagttcga cgccctcgcg
    83641 cgcgtcctcc tcggaatacc gctcgtcccg ccgcggctcg tctcggccgg cgccttctgc
    83701 atggcgaacc tcctcggtga ggtctggcag gcccagggga ccgaaggacg cctcgacctc
    83761 tcggcctggg aggggagccc ggacctcctg gaggtcgtcc tctacgggaa gagcggcgtc
    83821 gcgacgaagc ggaagatggg ccatctggtc gcccgggggg ccggggccga cgaggcgatc
    83881 gccgccgcgc gcggcttccg aagggcgctc tcgcgaagag gaccgcaggg gtccttataa
    83941 tcaacggttc ggatgtccgg aaccggcctc tccaacctcc tcttcggcct cctcgccctc
    84001 gtctgctacg ggggagcgac gctcctctac gtcacctggc tccggcaccc tcgggtctcc
    84061 gccgggcgcc tggcgacgct cctggccgcc gcgggagcgg tcttcaactt cgtcttcctc
    84121 tacgtccggt ctctcgcact gaagaccgtc ccgtacagcg acctcctcgg ctcgatggcg
    84181 ctgttcggcc tcttcctggc ggccatgagc ctgatcctcg agacgcggca ccacgaccgc
    84241 tccctcggcg ccttcctgat gcccgtctcg ctcttcttcc tcctccattc gttcctcctc
    84301 cccggaggga cgcctccgcc gccggagatg cgagggtcga tcttcgcgtt ccacgtcacg
    84361 ctcaacatgc tgagctactc ggcgttcgcg gtggcgtgcg ccctctcgct cctctacctc
    84421 gtcctcggcc ggcggcttcg gagcaagcgg cacgtcctga acggccccgt ctcgcgcctc
    84481 ccgtccctga gctacctgga gcgcgcggcg cggacctcga tcggcgtcgg cgtcgtctcg
    84541 ctcgcgaccg ggctgacgat ggggttctac tgggcctatc gcgtctggca tgagcagcac
    84601 cccgactggc tcctcgacgc caaggtctgg ggatcggtcg tgacgctggc cttctacctc
    84661 tgggtcttcg tgagggctca ccgcggcgcg gcccccgggt cgacggcgaa gctctcgatc
    84721 gccggcttcg tcctcgtcct cctctcctat accgccgtaa acctcttctt ctcgaaggtc
    84781 cactcgttcg cctgatgcct cccgagatcg tcttcgtcgg ctggagccac cagggggcgg
    84841 acctcgacct gagggcgcgg ctcgccttca ctccggagaa ggctcgggaa gcgctcgagg
    84901 gtctcttccg cgaacggatc ctgaccgagg gggcggtcgt ctcgacctgc aaccgggccg
    84961 aggtctacgg ggtcgccgaa gctccaggca gcatcgacgc cctcgccgag ttcttctccc
    85021 gcttccacgg cgtcgaggcg agcgtcctgc gcgagaaggc gttctccgga cagggggagg
    85081 cgacggtgcg gcacctgttc cgcgtcgcgt cggggctcga ctcgatggtc ctgggcgagg
    85141 cccagatcct cggccagatc cgcgaggcgc accggcgcgc cgcagaggcg gggaccgccc
    85201 gtgccgtcac gagccgcctc ttccagtcgg ccctcgagtg cggcaagcgc gtgaggaccg
    85261 agacgacgct cggggcgcgc cccgtctccg ttcccggcat cgcgctctcg ctcgtcggcc
    85321 ggatcttcga gtcggtctcc gactgcaaag tcctcctcct cggtgccggc gagaccgtgg
    85381 agctgacggc gcgtctcctc gcggacgacg gcgtgaaaca gatcacgttc acgaaccgct
    85441 cccccgagaa ggcgaaggcg ctcgccggct gcttccacgg ctggacggtc ccgtgggagg
    85501 agagggcgaa ggcgttcgcg gaggtcgaca tcgtcctctc cgcgaccggg gcgaccgagc
    85561 ctgtcgtcac gttcgacatg ctcaagccgg tcctgggcag gggacgccgg cgcggcccgc
    85621 tcctgatcct ggacgtcgcc gtcccgcggg acatcgaccc gaaggtcgag gagttctccg
    85681 acgtctaccg ctacgacatg gacgccctcg gagagctcgc ctgcgacaac gcgaaggacc
    85741 ggatggccga cgttccccag gccgagcgga tcgtggagga gtcgacggag cggttcctcg
    85801 gctggctcgc cgggctctcg cacgtcgaca cgctgaagac cctccgtggc cggctcgaga
    85861 aggcgcgtct cgacgagctg gagcgctatt ccggaaagct ctcgcacctc accgccgagg
    85921 accgcgccgt cgtcgagcgg atgacggaga cgctgatcca caagatcctc cacaacccga
    85981 cgatcggact gaaggaaggg gatgcctccg agcgcctcac ccgcgcggcc gtcgtgaggg
    86041 ctctcttccg gctcgacgac ggggaatagg ggcctgcacg tgaaaccgag gaatgaaccg
    86101 ccacgaagcc gggggggccg gagggcggcg gacagcgccc cccggcgagg gtagggcgcg
    86161 cgggtcgcgg cgcccccgcc gacccacgag gaaaggacga cgatgagcca cggacgaccc
    86221 ctgcggatgg gcacgcgcgc gagcctcctg gcgcggacgc aatcggggca cgtcgccgcc
    86281 gcgctgacga aggcaacggg gcgcgcggtc gaggaggtcc tcgtgaggac cgagggggac
    86341 cagctctcga
//
LOCUS       DAOONL010000003        83164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_137_length_83164_cov_19.290281, whole genome shotgun sequence.
ACCESSION   DAOONL010000003 DAOONL010000000
VERSION     DAOONL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 83164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 83164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_83164_cov_19.290281"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1282
                     /gene="lat"
                     /locus_tag="PLP50_00815"
     CDS             2..1282
                     /gene="lat"
                     /locus_tag="PLP50_00815"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HPA50114.1"
                     /translation="MDGYHVVMDLERSHGSFIYDSRHDVEILDLFSHFATIPVGYNHP
                     SLKEPSFLEELRRAALTKPANSDIYTEEMARFVETFARVAVPPSHSEHLFFVEGGSVG
                     VENALKAAFDWKVRRNFRKGYRREVGQQVVHFEQAFHGRTGYSLSLTNTADPRKTMYF
                     PKFRWPRIVNPKLRFPVDAAELERVEKVESLAIAQVKQALVENRDDLAALIIEPIQAE
                     GGDNHFRTEFLAALRQLCDENEMLLVCDEVQTGVGLTGSMWGFQTMGVVPDLFAFGKK
                     MQVCGFASNGRILDEAENVFTVSSRINSTWGGNLVDMVRARKYLEIIEEEKLVENARA
                     VGTVLLEELAALSAEFPGKVTNVRGRGLFIAFDLPDGATRGNVLSTMLQKHHVMALAC
                     GESSIRLRPALTLGRDEALLGVKRLRAALAEVLG"
     gene            1302..2051
                     /gene="mtgA"
                     /locus_tag="PLP50_00820"
     CDS             1302..2051
                     /gene="mtgA"
                     /locus_tag="PLP50_00820"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPA50115.1"
                     /translation="MTAARGASCRARLLALGAAALAALLLGVFVPVPRIVALRFGPPS
                     STAFLEARRERLRAEGRNARIDRRPVPLEKVSPHLVTAVLVSEDARFFEHHGIDWSAV
                     KAARTRNRRFPKRRPLGASTITQQLAKNLFLSPSRSWLRKGREAAVATAMELLLPKRT
                     ILEHYLSGIEWGERVWGCEAAARTYFGRPASALTREQAAWLAAMIPGPRVYLAHPIRH
                     QRRAARILARMRRAGDVRPLLLEEADDPPAR"
     gene            complement(2092..3183)
                     /locus_tag="PLP50_00825"
     CDS             complement(2092..3183)
                     /locus_tag="PLP50_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPA50116.1"
                     /translation="MKASPGLRQLPDGRWRFRLYTAGRKDGPRVQVTLPKGTTRTEAE
                     ATYRAAVAKAAARRGRPIPRRLTFADAAAEYLGIQRGRLAPGTLVNVENIVNRLVSLF
                     GDRRVEDLRPSDVVAYQAARVDAGVMPSTVNGEVVYLLAIVRKVLALGWIERDPLPRG
                     SFDRLSTPSPETVFFRAEEWAAFMRKLEELRPDAVPAFRALLLLACRIGELSGLTWGS
                     FDLEGGRVTIAQPKRRGRVKTLPLAGELREVLDSLQRGLPAAPVFTREDGSPWDGRQL
                     QRLFYRVRDAARLRKALHPHSVRHTAASWAVQSGVPLLSVKETLGHANISQTVRYSHL
                     ETEHLRGAVEAVSTVEKSGRRHRGATDRG"
     gene            complement(3180..3770)
                     /locus_tag="PLP50_00830"
     CDS             complement(3180..3770)
                     /locus_tag="PLP50_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50117.1"
                     /translation="MKASPGLSREEIAARADLTGIPDVLRERAIDALSERNIVGFFLK
                     ADNLLSLDLLWRNRVLFESLGIFEAALLDAYQAPRTNCLHVPAARLRGLFARADRRRL
                     REAGAPLPTGGPFTLFRGVAGNGPARRVRGLSWTSDRAVAEWFASRARLMGLKSPAVY
                     RAVVPTRHVLAYITEREESEFLVLLPPSIRVEREAA"
     gene            complement(3767..4003)
                     /locus_tag="PLP50_00835"
     CDS             complement(3767..4003)
                     /locus_tag="PLP50_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50118.1"
                     /translation="MSREVSEKLDQLAELLRGLPPERVDLLLRPSERLTVYLTPQQKD
                     DIRAAAEHYGLTLSGFAVRVLDAVATAHRERVRK"
     gene            complement(4003..4710)
                     /locus_tag="PLP50_00840"
     CDS             complement(4003..4710)
                     /locus_tag="PLP50_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPA50119.1"
                     /translation="MKSATKSAVGYVRVSTERQASEGVSLDAQEARIRAWAVAQGLDV
                     AAVHVDAGLSGGKAANRPGLQAALADACKRRAPLVVYSLSRMARSTKDALAIAERLDR
                     AGADLVSLSESIDTTSAAGKMVFRMLAVLAEFERDLVSERTTSALAFKRRQGERISGK
                     IPYGYRLEAGQLVAVEEEQTAIRLMIDLREAGLSLRAIAEELERRQIKRQNGAHVWPS
                     NVVLMILRRAAKLREAA"
     gene            complement(4939..5292)
                     /locus_tag="PLP50_00845"
     CDS             complement(4939..5292)
                     /locus_tag="PLP50_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50120.1"
                     /translation="MVMGRHYNRTKKDIGGTRKASPEKQDLQAEKTSYRLAKAYGVGH
                     DTIEKAGKFAAAVETLKAVDPEIGYHAGPDGWRPRLRDLAESCGIRASRLRRTPGAIR
                     RTRPPWRPVRPRVRP"
     gene            complement(5549..5797)
                     /locus_tag="PLP50_00850"
     CDS             complement(5549..5797)
                     /locus_tag="PLP50_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50121.1"
                     /translation="MSGCVFRFDMDTSSPHYCPNEAHSGMGGYCLEHARENPNDPLRC
                     LMQIPPGDPGNELGTTTLRCKGTRVYGHAPYCAAHATA"
     gene            complement(5810..6016)
                     /locus_tag="PLP50_00855"
     CDS             complement(5810..6016)
                     /locus_tag="PLP50_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50122.1"
                     /translation="MGAPYREVLADGYDVATRGQNVRPRRVINWTDRQVCRAILAPAS
                     PGAISTNCPNKPVNDLGFCAAHAR"
     gene            complement(6016..6600)
                     /locus_tag="PLP50_00860"
     CDS             complement(6016..6600)
                     /locus_tag="PLP50_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50123.1"
                     /translation="MIASLFKSGAEKIAARLANLETEAAALAKRAEEARAAEAEAQDR
                     LVEDLAEGLPVDDWRKKRDAAEKKAADAERDLAAAQRAAELVRAKLEEAREAERLGDL
                     RRRYADGRERYADVFRDYLAAGKAFSEALGRLEGYRSEMNGIFMALRAAGDRETAQVG
                     IPTAENVLSDDLGHAEGRERFPIDVPIWPSKGGA"
     gene            complement(6597..7004)
                     /locus_tag="PLP50_00865"
     CDS             complement(6597..7004)
                     /locus_tag="PLP50_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50124.1"
                     /translation="MSNAPMTFFSPGRPPAGAASSRDAATPLRADIRDEIRRERAALA
                     DPVRRVTREIVREELAAANPTPAEKVRRAAQEAHALSADPAERKRIALAAVRGLGVGL
                     TSLPLDDLTRATSEAVGCTYAEAALALTTEDAR"
     gene            complement(6997..7326)
                     /locus_tag="PLP50_00870"
     CDS             complement(6997..7326)
                     /locus_tag="PLP50_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50125.1"
                     /translation="MADHDSLSLAAALLASGAKPPRIVETLSELYAVHAETPVPHYVL
                     SPQLARFFADLEKKYAEDPNRQEALRRVALDVVAEAKRGQPGAIEVRYVNDWRDDRRE
                     PKGDPDV"
     gene            complement(7329..7682)
                     /locus_tag="PLP50_00875"
     CDS             complement(7329..7682)
                     /locus_tag="PLP50_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50126.1"
                     /translation="MSDGRDPSGKFAPGNRAGALRKPRYPGIRFAAWCREVLADEDRR
                     ARLVAKVDREIDGDGPAPVLTKILGYAFGEPKQVVEAEFKTRAAKLAQIVGVPVETLL
                     AEANRLSREAGYEEN"
     gene            complement(8099..8293)
                     /locus_tag="PLP50_00880"
     CDS             complement(8099..8293)
                     /locus_tag="PLP50_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50127.1"
                     /translation="MNPLIISVSTAWEASGLSLAAFGDLVDALVSSGRAVRHRDLLVF
                     PNLKTAALVLSGRPRRRARR"
     gene            complement(8290..10554)
                     /locus_tag="PLP50_00885"
     CDS             complement(8290..10554)
                     /locus_tag="PLP50_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HPA50128.1"
                     /translation="MSERLTAALEAHGSRGKGSAWQCPAHEDRSASLSVSQGRDGRAL
                     VKCHAGCDLEAILGALGLTVADISPERDERRGSKRIKAVYRYTDETGALLFEVVRFEP
                     KDFRQRRPDPAGKDGYAWNLNGTRRVLYHLPEVAEAVRAGLPLFLVEGEKDVDNLRAA
                     GLRATTHAGGAKSWRAADYVETLRGARVVVVPDDDEPGREHGRAVAQSLRGVAAEVRV
                     LNLGRPGVKDASDWLAAGGTREELESLAADAPTADALARVDGVDVLDSLTRFVRRFVV
                     VTPEQAQLVALWTAHTWAFEASDQTPYLAILSAEKRSGKTRLLEVLGVVTRAPWHAVQ
                     PSEAVLYRKIEKDRPTLLLDETDALFSQRGDSRMEAVRALLNAGSRRGVTVPRVLDAK
                     SDKLVDFSVYCPKALAGIGTLPETVADRSFIVEMKRKRRTDSAERFRLRDVEKDAREV
                     RARLEAWTTANLGKLKTARPSLPRELGDRAADGAEPLLALADLAGGPWPERARRAIVA
                     LAGERAEEDESDGVQLLGDVRRVLALLVRTDGTIRTEKLLEELRALPESRWKKFGPRR
                     EEVDALDLARLLRPYGIRPMRIREGAETVRGYAVPTFADAFERYLSESPQSEESATPA
                     TPPQSDNHAGQSVAPYPPHYPPRKPTPSPEAESCGGSCGGSPATIPSDNRAGCGAVAP
                     VAGQSEHADFTYDADGLPRERWRDGTCPVVEDEEAPEPGSLVRCGRPLHPGAPACREH
                     AYGPFGRPAAEVAA"
     gene            complement(10607..11449)
                     /locus_tag="PLP50_00890"
     CDS             complement(10607..11449)
                     /locus_tag="PLP50_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPA50129.1"
                     /translation="MNAPGLLVAVDRIDALPGQPRRLFAGIAELAGSIREHGVLEPVV
                     LRPVGERFQIIAGERRLRAARLAGLAEIPAVVRDVTEREAFELALVENVVRENLTPIE
                     EAEAFRRLQDVGKTQSEIGRLIGKTQSYVAQKLRLLTLPDFLTVFLSSGTLTEGHLRQ
                     LLRLRDIYPEDHSDLVGDFTEEAVEAYPQIIYSVLRPQASEGAAWASVPPVAVDALHL
                     LNGYVTRHRAYGPPDWRKAEVPAWAVSALWFAADSVRSSRSVAGLSDAIDQWASDLCD
                     EVTA"
     gene            complement(11446..11694)
                     /locus_tag="PLP50_00895"
     CDS             complement(11446..11694)
                     /locus_tag="PLP50_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50130.1"
                     /translation="MNAPVPWRRLSDVLLEAGADPVLREVLEAGRHVDAEPGDGAAAF
                     LLNGETILLLATPDGMTVRRNAPADEVARFLASVREAA"
     gene            complement(11691..11840)
                     /locus_tag="PLP50_00900"
     CDS             complement(11691..11840)
                     /locus_tag="PLP50_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50131.1"
                     /translation="MNLYRAAGRIERLAEAIARELDVHPTSALEFALMSLVMNDPAVW
                     PKETR"
     gene            complement(12102..12308)
                     /locus_tag="PLP50_00905"
     CDS             complement(12102..12308)
                     /locus_tag="PLP50_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPA50132.1"
                     /translation="MAIPRTRERFDQKLMARLRDAAASVGVSEAALRSEIRLGRLEAR
                     RMGRAVLIPVDELRRWAADLPPAA"
     gene            complement(12456..13106)
                     /locus_tag="PLP50_00910"
     CDS             complement(12456..13106)
                     /locus_tag="PLP50_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50133.1"
                     /translation="MVKTTKARPTKAGTTLSAAERAEVLELAEILKDLGSSPTELLER
                     AMKAEHLLRSSARGAVLPYLREFGALGLYAPSVLAAIVRASTVLSSLPDDMRDGSRTF
                     AEAKKLLLGVTQALFAKHASTVEMDRRSRAREVLSLAQAVQTEAERLARSKPATFARS
                     DGKPNVSAVATARAKLASGAGLSVDALQLRIGRAAEAFPDAAWPEIMPRPPKKRQA"
     gene            complement(13212..13802)
                     /locus_tag="PLP50_00915"
     CDS             complement(13212..13802)
                     /locus_tag="PLP50_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50134.1"
                     /translation="MTETSIPETLANLAANVAAQHDEMTAAVEEERAAEERLLGQLVE
                     AVRPALRALSSRLLASERTFWPDNVSTATEKDYHDERGVRLAGSGPERDHPRANDGGI
                     EGTDLVLLEDGTFARLDWSGSWSRWQGAASHEDSALTRISLRDVVDGWDVDQLAAALH
                     ERLEAQMAGRAPKRTASARERAEKLRAVSSLLGARS"
     gene            13966..14808
                     /locus_tag="PLP50_00920"
     CDS             13966..14808
                     /locus_tag="PLP50_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPA50135.1"
                     /translation="MSRHPRLLAGAALLLAPLLLASCGPKASEIRLKEKKLTVYGLDR
                     TVSVGGDVVDGKGEIVPGFMVAWESSNPKVATVNPQTGSVKSVSAGKARLTARLDKPP
                     LEAHVALEVIDIASVNVSPLRMTLAGPAGTSFPLVLETRASRGALVPMTAVWVSSDPK
                     VATVDASGIVTSVAEGKTTVKASIGDVSGATDVVVVLRSIETLEATPLNIPLKVGEAL
                     DVTLVARDAQGQPIPDVAVTWTSSDPSVATCSSGRVVGVGPGSTTVRAVCGARSAEVA
                     VIVF"
     gene            14805..15680
                     /gene="amrB"
                     /locus_tag="PLP50_00925"
     CDS             14805..15680
                     /gene="amrB"
                     /locus_tag="PLP50_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPA50136.1"
                     /translation="MTEAVRLPAVAGLFYEGGARALRADVERYLAGAASPPRPVAAWR
                     ALLLPHAGHVYSGAICGAGLAAVEVPSTVLLLGPNHHGAGTGVALSGAGAWRTPLGDV
                     PLAGGLAEELRNASRAIRTDEAAHRREHALEVILPFLQVARPGLSIACLTLGEPDLSV
                     CLEVGRAVAEAVTRVEEQGERVGIVVSSDLNHYLPRERNREKDDRAIDALLLGDPSEL
                     FSRVLLRERISMCGILPATALLAALRHLPPTRPRLVARGDSGDTSGDTSRVVGYAALL
                     WETRTGTNGSERKEC"
     gene            15680..17164
                     /gene="glgA"
                     /locus_tag="PLP50_00930"
     CDS             15680..17164
                     /gene="glgA"
                     /locus_tag="PLP50_00930"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPA50137.1"
                     /translation="MRVANFSAEVSPFAKTGGLGDVVSSLPKALAATGHDVDVWVPFF
                     LEAAEWFRRRGTWPEQEVEPFDVSILGATYRVGVLRATLPGSSVPVHFVAHDPLFHRP
                     GGLYAKNDQGVDDGIWRFCLFVRAAIEALKRKGDRPEVIHAHDWHPGLAPMLGAWSSW
                     RDPWFDEVASVLTIHNVGYQGIYGPDQVPALGLPPEAWSGGLLEHSGSLNMLKGAIVA
                     ADMVTTVSPTYAWEVRTPEGGAGLDGVLRLKAARLTGILNGIDRQEWNPSVDPHLASR
                     YSAEDLRGKLDCRGDLCRLAGFEAGDPAMIVGSVGRLVPQKGYDLLLEAAPELLRRGV
                     RLVLLGSGEPALEGSMRLLEAHYPGRFKGFVGYDEALSHKIQAGSDGLVMPSRYEPCG
                     LTQMYALAYGTVPIVRRTGGLADSVIGLHGENLEWATGFHFDDPSPHAVAAEVLHAQS
                     VFFRREAWSRLVRNGMKADFSWGRSANDYELVYRRAREVRGLPW"
     gene            complement(17246..18064)
                     /locus_tag="PLP50_00935"
     CDS             complement(17246..18064)
                     /locus_tag="PLP50_00935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPA50138.1"
                     /translation="MPVSRPLDAAVEAARRAGDLLLGHFGSLDPSEVEEKGRNDVVSR
                     ADRESEALVKELLLGAFPGDLFIGEEGGVTGSNGGAPAWIVDPLDGTANFVRGFPHWA
                     VSIGRTAGGRDGELVLGVVWDPVKGDLFVAEKGSGAFRNGRRVGVSPRDGLAGAFLAT
                     GFPFRVQSRIDTYLRVFKAVFLEAQSIRRAGSAALDLAYVSCGVFDGFFELGLSPWDS
                     AAGAVLVSEAGGLVTDFEGGGAWRERGNILAAGPGVHAALARLVAGLGIRDEQL"
     gene            complement(18064..18456)
                     /locus_tag="PLP50_00940"
     CDS             complement(18064..18456)
                     /locus_tag="PLP50_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50139.1"
                     /translation="MPPSRAKRVVLVDDSSAYAASWRAIFAERYGGKVVFEAYQDPVE
                     ALSHLGGDVDLLLVDLEMPVLDGRSVARLAKERGVSEKRIVIVSGHDADELHRLFPQG
                     ACLAVINKTEAQQQSAFLMILDSVIQRH"
     gene            complement(18522..19838)
                     /locus_tag="PLP50_00945"
     CDS             complement(18522..19838)
                     /locus_tag="PLP50_00945"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPA50140.1"
                     /translation="MSDAPVLISGGRVLDPSRGLDATLDVLLEGGVVARVDERIAPPR
                     GAETVDASGLVVAPGFVDLHVHFREPGFEWKETIASGSAAAAAGGFTTVCCMPNTDPV
                     VDDAPTAAFVQKRIRETARVRVLPIGAVSKGQKGVTLAEIGLMKAEGIVAISDDGRPV
                     VNGDLMRRALEYASMFDLPVVVHEEDPNLVGGGQMHEGWASTRLGLPGWPAVAESTMV
                     ARDVELAALTGARLHVAHVSVRDSLAAIRRARAARVPVSCEVTPHHLTLTDEDLARSG
                     YDTRYKMNPPLRDEADRQALVSALADGTIDCVATDHAPHHADEKAMEFASAPFGVTGL
                     ETALGVLVDALVAPRLLSLSRLVEAMSTAPARLFGLPYGTLAEGVPADVVLFSTARTT
                     TFSSFRSLSANSPWAGKTLRGRVERTFVSGREVHRFGAAAAPEAAG"
     gene            complement(19980..20942)
                     /locus_tag="PLP50_00950"
     CDS             complement(19980..20942)
                     /locus_tag="PLP50_00950"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPA50141.1"
                     /translation="MSRKPETPADPASFTKKDLLDIESLSKAEIQMILDTAESMAEIN
                     KRTIKKVPTLRGRLVANLFYENSTRTRFSFEIAERRLTADSISFAAATSSASKGETLL
                     DTCRNLEAMAPDAIVIRHQASGAPHFLARNLKCAVINAGDGTHAHPTQALLDAFTIRK
                     AKGKLDGLRVAICGDVRFSRVARSNLALLPKMGAEVTLAGPRTLLPPEIERTGARVTT
                     RIEEAVDGADVVMMLRVQKERGGGLGFPSDREYFELFGLTPERRKLARKDAIVMHPGP
                     MNRGVEIDSAVADGPGSVILDQVEAGVAVRMAVLYLLIGVSGDE"
     gene            complement(21080..21631)
                     /gene="pyrR"
                     /locus_tag="PLP50_00955"
                     /pseudo
     CDS             complement(21080..21631)
                     /gene="pyrR"
                     /locus_tag="PLP50_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
     gene            21812..23770
                     /locus_tag="PLP50_00960"
     CDS             21812..23770
                     /locus_tag="PLP50_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPA50142.1"
                     /translation="MLTDALAISLPIAGVAIRTLVARASPLPPDRPALFLFSGFVQDL
                     AAGLLLALLARGLRLAGARPGAAAAVTAVPFAILLVGHAAWAEAITFFGHPPRREDLE
                     VALNAELLTRSADARALGRLASFLAVGALGTVLVSHALARWRRASTRQIFVGATLAGG
                     LALLIHQLEPVPTANGVLFAALDFAREPRPETGRGMATVPPPHSAPEAIRRLAPGRAW
                     VDPHFPLAFEPSARRAEAPRVGEGVRPNLVFILLEGVRAREISCYGGPVPGLTPNLDR
                     LAREGTRVARAYSPGTFTPQGEAAYWYGLLATPGSLLPVQNPATPLLGLPEILGANGW
                     RSFLWIHNGDESFYRRDRFYLSRGFRMVDGRDFPRSAVRTNWGCSDRSLARLAVDSLD
                     RTREPFAAMLLTVSNHHPFQVPSDARSVAPIPWTEERGWFSPAPGLPLVGRHTVPMMK
                     TIHYTDEALGDFFREAASRDWARRTIFVISGDHGLPIVPLGGLGSAHELAELRHGVPL
                     VIWSPLLPGGRVLEGPATLADVPPTLLGLLGIDSPRPFAGVDLLDPDLDLADRPLPLY
                     DEEARRVTVLRGRLAYHARLEPPRTSPDWTLGNELLVDTVRDPRGVENAADLLPHETA
                     RMRRDAEVFVDVYPWLALAGRTGLPPER"
     gene            23781..24815
                     /gene="queA"
                     /locus_tag="PLP50_00965"
     CDS             23781..24815
                     /gene="queA"
                     /locus_tag="PLP50_00965"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPA50143.1"
                     /translation="MNVSDFDFDLPPEAIAQRPAPRGTSRLMTLDRATGATGHRSVAD
                     LPALLRPGDLLVVNDTRVVPARLFGLDETGRRTEFLLVEKTEAPSAWDCLAKPGRRAK
                     AGRTFDFGAGLRGTVLSKGESGHYRVAFSGTPLSEALPLVGTTPLPPYIHRDGGVADA
                     RDAVEYQTVYAREPGAIAAPTAGLHLTPELLDALRARGVEVCSVTLHVGIGTFKPVKV
                     ERVEEHRMDTERGVIPEETFVAVRRAREEGRRVVAVGTTSVRTLEASARAHGGDVAAG
                     PFETRLFLVPGAQFQVVDALLTNFHLPRSTLLMLVSAFAGREQVLAAYREAVRSGYRF
                     FSYGDAMLVA"
     gene            complement(24861..25421)
                     /locus_tag="PLP50_00970"
     CDS             complement(24861..25421)
                     /locus_tag="PLP50_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA50144.1"
                     /translation="MSSRRLIVAAFIVAGCLASPVAVAAPAAAVVPAAPPAEAVLAAA
                     AKAARGEKKNVLVLFHASWCGWCRRLEAVLATPDVAAVVSRHYVRTELTVFERGERKE
                     TENAGAETLLDSLAGSDAGLPFVAVLDARTRQPIATSNVAGPGTNVGFPSKDEEVDHF
                     VRLLRKGAPRMTGEEERTIRAAFPTR"
     gene            complement(25478..25801)
                     /locus_tag="PLP50_00975"
     CDS             complement(25478..25801)
                     /locus_tag="PLP50_00975"
                     /inference="COORDINATES: protein motif:HMM:NF036906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HPA50145.1"
                     /translation="MTATTKKAPATVDVRAYPALRDALRGYLHEDWTTEYADSEDAAR
                     AFRRDAADDEKRNVAPEAKKLRAALEGMEVRDLRKLLGAGFGTGWRPSTAAEAERVLR
                     ILAGE"
     gene            complement(25798..26289)
                     /locus_tag="PLP50_00980"
     CDS             complement(25798..26289)
                     /locus_tag="PLP50_00980"
                     /inference="COORDINATES: protein motif:HMM:NF037816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50146.1"
                     /translation="MVLRRLVSRLRPIVPVLLALAALLGATAPADGAQKRDLLRDEKR
                     GGHTLARHVGQSDAQLRERLRRERRLSAASTFTDRATAEEVVADAIRGSRKRIERWTN
                     REGSRANLVIDWPGDGRKVVGRSLRRGAKEPVPCTRAVVVLRWNVDAEIYYVLTAYPE
                     ASR"
     gene            complement(26418..28844)
                     /locus_tag="PLP50_00985"
     CDS             complement(26418..28844)
                     /locus_tag="PLP50_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPA50147.1"
                     /translation="MSDNPRENRWLWDFGGFRLDARARLLLKDGLPVPLTPKAFDVLV
                     HLARNAGRAIPREEILAAVWPSTVVTDASLTQAVFLVRRALGEAEGTGMLETVPRFGY
                     RLNLPVTSAEAGGPEAPPSPVPLPSTGPEETDEAAEPLSGASPDPPAASARRPAASLL
                     GVLVAGTLAAAAIASILLRSPARGGSEGAAAGVEPLLSLTREIAVPPDAQSVLGSLDG
                     TIVLSARSAYDLLPADGAHPAVRVPLERDEVLAEPLGGGRVLLVRGRRVVARHPTKPE
                     EADLGLLPPDAPGPRPGRVRCSRSGRNLAVRGDEALEVFERGGSGWSRRLRLNLPFVE
                     GEVLDVGERLVALAQGGGGPVRAFGLPEGNALFVTPFAEQRVSALAVDDALGRVAVGG
                     PFDFVAVFSAGDGLAPKSLPRRGWTYGLAWVGDAPTLLASGLEGITAYRSDEEPAAIL
                     APVSPGGGLHVDSDAILALVPRRQRLAVVSYAGITPTARVPAGGRPLWSAEHDAAGMT
                     VFAGGRDGELWSLDTASLAILRASAHTDGIPSMVRDGDLLATSSDDKTVALWGLPGPS
                     LKLRTRAHDFLVNDLTVVDGPDGRELVTASSDGTVRRWRWPSLDPVAIVDAASLLGRK
                     VSLHAVAPLPGSLRILVGTWSSSLLELTAREGRWSVKEHPVPSRAVYRLAPVPRLGLY
                     VAAGIEPSSLHLFDLASGTLSPLDAAGLDGMWVVPVPGEDDVLVVGLDGVSRYSFSAP
                     LPDAAGRRTVTYRAWSRRRTGAGLQTATLLPDGTLWAGTVDGELLRFDAGQLAGPPLL
                     TRALDLAPPR"
     gene            28930..30201
                     /locus_tag="PLP50_00990"
     CDS             28930..30201
                     /locus_tag="PLP50_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50148.1"
                     /translation="MRRTLRTRPTFLGATLAGAALAVALPSAAANVLQNGSFATGLSG
                     WKMTAPASWEAEGAAGPGSGSARLGGNPGIVGALSLSQCAAVTPGTAYDLGATALLPH
                     SPEAQGGISIRLVWHSWPGCEGATLGLAPALDFPHVAPAGWTRRSLERLRAPYGAASA
                     SLLVVPWSSGSESAFSAFLDDVELSPSSMFETLTLPTAASVDGANGERFQTTLVLTNV
                     ALFTRRVDVTLRCPSSQPCGTSAVSLQLAPGETRVFADLLLDVFGKRNGAGAVEVTYE
                     ATPGPIVVSARAATVHAERPGNGMALPVLPTSAVRSSATFFGLTDGRAGAGGTRVNAG
                     VYNPSDTPIFVGFSVHNENGDVRNTVSRSIAGREWAQIDDLFSRAGLGSATPGSYVTF
                     QAEAPVFPFLISIDNRSGDPTFLEPTESFLP"
     gene            30198..31451
                     /locus_tag="PLP50_00995"
     CDS             30198..31451
                     /locus_tag="PLP50_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50149.1"
                     /translation="MRPSAAFLHAFAVLLAAALPASASGAENLVANGAFREGVSDWSR
                     IDVVSYSAMGAATPGSARITLPSRPEGGDAMLQCIAVEGWRLYDLGAAVRLPEWPEHG
                     GGVSVRLRWHALPNCQGPSLRGAPSLDFSYTEPAGWQVKESRRIPAPQEAVSALVVVV
                     ARAAGEEPYPIFVDDVVLRRSVEEQDAFLPTAASVRGAKGERFETDLWVYNPAPAERM
                     FTLRLWRDGQPTTPVVLRVGAKQTRHLSDVLLSDLGQRDVAGAVQLSFDPGGGPMHFS
                     ARVVTVNPESPGNGTLLPVLRRDEARTDALFPGLSGARDDLTGAFRVNAGAFNPHDAP
                     VEAVFWLYDGDGTELGTFTRTLAPGGWLQVNDVFREVGAEGVSTEGAVLAFNARLPVF
                     PFVIAVDDRSGDGTSIKARDYPVLR"
     gene            31522..32481
                     /locus_tag="PLP50_01000"
     CDS             31522..32481
                     /locus_tag="PLP50_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50150.1"
                     /translation="MNLSGGFSVSPAMPLPRSRWLRILGVFLVLAVGAAGLRTALEPE
                     ETPAPGLTLPEAERDRIGALARASAADAGRRLVDLSGVDVLLVGEEHFYVETQAWLAR
                     LLDGVTNRRIALLLELPRGLQPEVDRWVATGRSARLEEAIRSRDALPFGDLLAWAHQN
                     RSRLSAVVAMDEDGNRIFWNRVFLRDTRNRTMADAVLSARRAGPGDLVVAYGGQMHML
                     LAGRYRYDRKDRVPAGARLLREGVPRERMRSVLLSGEGKAPVCEAWAGPGALDMADAI
                     GAEPWALFVDYPLFRVGASRDLFDFFVHLGPLTRVSRPEAGSR"
     gene            complement(32495..32827)
                     /locus_tag="PLP50_01005"
     CDS             complement(32495..32827)
                     /locus_tag="PLP50_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50151.1"
                     /translation="MYGSGSPLLAFLSSVARQLPELAVLVVGFVVVLGRKPAHPKASS
                     LAAAGLAVALATSLGGAAFYAAAPTLASNPGALGALYAVSGFAFSALDAAALGLLVAA
                     VVADRAPR"
     gene            32902..33147
                     /locus_tag="PLP50_01010"
     CDS             32902..33147
                     /locus_tag="PLP50_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50152.1"
                     /translation="MAAPSPSDRLLEILAAKIAEETGLDPALAREVAPAALASDSVDE
                     GEPIALRDARGHVVARIPFSLVEEAFDDLDDEEDGER"
     gene            complement(33301..34641)
                     /gene="glnA"
                     /locus_tag="PLP50_01015"
     CDS             complement(33301..34641)
                     /gene="glnA"
                     /locus_tag="PLP50_01015"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPA50153.1"
                     /translation="MATKAIEILKTCDKEGVRFLRLQFTDILGIIKNVEVPRSQFEKA
                     LDGEIQFDGSSIEGFTRIEESDMGLVPDLETFRIFPWSHTNGKVARLVCDVITADGKP
                     FAGCPRQTLKRQREKAKELGFSLMTGPEAEFFLFRRDASGDPVVDTHDSGGYFDLTPV
                     DRGEDARRDIVVALEAMGFEVEAAHHEVAPGQHEIDFKYGDAVVTADKVTTFKLVVKK
                     VAMDHGLHATFMPKPIFGVNGSGMHVHQSLMTGTGAGMKNAFFDAKAPYQLSKTAMYY
                     IGGVLKHAKAIAAVTNPLVNSYKRLVPGYEAPVNVAWSEKNRSPMVRIPARRGMGTRC
                     EVRMPDPACNPYLAFAVMLAAGLDGIKNRIDAGEPVNENIFEMSEREKRRRKIEQLPA
                     NLSDALDCLEKDDVVKNALGEHIHQHFVEAKRAEWATYISRVHPWEVEEYLQAY"
     gene            34815..35447
                     /locus_tag="PLP50_01020"
     CDS             34815..35447
                     /locus_tag="PLP50_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPA50154.1"
                     /translation="MTAPTRENAWALLTEFTASQALRRHALAVEAAMRALARSAGVTD
                     EAEIERWGLVGLLHDFDYEKYPTEADHVFRGMEILRQRGWPEDLIRAVGGHAFYTGIP
                     RETPMEKAILAADELTGFVGACALVRPSKRIADVPVESVMKRLKEKAFAAKVDREYVR
                     RGAEEWGLPLPELVAIVLASQVPLADALGLGGAPAPDLPDAPCPPPPPAP"
     gene            35512..36900
                     /locus_tag="PLP50_01025"
     CDS             35512..36900
                     /locus_tag="PLP50_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA50155.1"
                     /translation="MRALLIENALVAGMRSADDLHPRGAVWAEDGVIRALGSEARAAA
                     AAAAARGEAVERVDAEGLWLFPGFVQTHVHLCQTLLRNGPDDLPLLPWLKTHVWPGEA
                     AHDEETLFLSARLGLAELAAGGTTAILDMGTVRHTDAVFRAAQASGLRVTSGNALMDD
                     AAMNPPELFAETSEALEEGERLLARWHGAANGRLRFAWCPRFAVSCTDRILEEAATRA
                     KAQGVLVHTHASENVDEVALVRERSGRANIEHLREVGIGGRGTVLAHVIHTSEEERRL
                     LAADGTGVAHCPSSNLKLASGICPVPDYLGRGIRVTIGADGAPCNDRLDVFSEMRHAA
                     LLQKIAAGPGALTAWQVVRMATAEGAGALGLGDVCGTIEVGKRADLVLLDPSAGFALP
                     TSWRDDPYGPVVFSFDRSNVVETRVDGEVVFHRERRGARVLAPTAAEIESAVARLRES
                     RVRNGRRGAGAS"
     gene            36954..37421
                     /locus_tag="PLP50_01030"
     CDS             36954..37421
                     /locus_tag="PLP50_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPA50156.1"
                     /translation="MAARENAARLKSIVVGTDFSICAARALAHAVSLAQLSGAKLHLV
                     HVLLEPVQALDVAAALPYPDVEVRREWEEAARVKLDREVKAAEKRGVPTTAEIRWGRP
                     SDTIVDTAARQKASMIVLGTHGRSALEKLLLGSTAERVLRLSPVPVLTVREKR"
     gene            complement(37473..38300)
                     /locus_tag="PLP50_01035"
     CDS             complement(37473..38300)
                     /locus_tag="PLP50_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50157.1"
                     /translation="MERLNLVLTIPHGSVIATSLGVEGLAHHLSPGSGRHFHGRGIYV
                     DLALGEAGAAFSFLEEGGWRDTAGDTARAIQEVRSGKRTKTALSSNAFTITPIEAYRR
                     VLLVKTGGQALSMGPVSDVARFGPAPCRDSMAPAEVAQAIGAAEPARREPRLYAIFAP
                     LELLVLSNLTPVEYAWYATHRPGKVFRQVCFAELRTDQPQLAASSRYDNARQEIRENP
                     KKKTKTVAVQDLLNAVSFEEWVGFGDATAGGLYFGDRTRLTRTPFPVEIPHAWEKAD"
     gene            complement(38344..38922)
                     /locus_tag="PLP50_01040"
     CDS             complement(38344..38922)
                     /locus_tag="PLP50_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPA50158.1"
                     /translation="MSIRDTPVLPIEAIGEEFRSGTKSYERPGPIDGVAIQRLPRFVD
                     DRGFFQEIYRAKADHAGSESLAAFFRDVPVAQLNYTIVNAENHVKGLHYHLKQQDVWF
                     CPHPSKAKFVLFDVRKASPTYLRTQVVVAGDGQDLLVRIPEGVAHGYRPLTNPCALFY
                     LVTRTFDVNDPDEHRIPWDHPAVRDLWEVPNG"
     gene            38971..40239
                     /locus_tag="PLP50_01045"
     CDS             38971..40239
                     /locus_tag="PLP50_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPA50159.1"
                     /translation="MPKRIIPVSSGKGGVGKTTVATNFALSLSRFAPTILVDLDTGTS
                     SVRSSIGVPVAHDLYHFFRKGRRLDECVTRLPDSWDPDGLYRGFGFVAGPLHLIEEVT
                     NFGPEKKARLCDAINELPADYVILDMKAGLDANVVDFLPWSNSGILVFTPHLPSATLA
                     ASDIVKAILFRKLRIVFSPSSPFYAQVKDPRTTTLLVNDLLDEVEDVYEPGLPNLDAF
                     LVDLAAALGDHPVLDQVARTVEHFRVHYVLNSFNGVDEAFDTAVKPFVENLVSTVSER
                     MTLTNLGWVVRSDEIHQGNCRKRPVLLAPPDGRPASRPTRGERELDALIRETAGLAAD
                     PRPRKPVLPVPTRPDPESALARELDALNRMYHRRGNRDDPRDNFDYLTARALHLLERA
                     RPSEFGTARLVGPVEILRSFFPGGAARPAG"
     gene            40236..40496
                     /locus_tag="PLP50_01050"
     CDS             40236..40496
                     /locus_tag="PLP50_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50160.1"
                     /translation="MKVSGRAAAVGLAILAGAAVLASRGRLRELGLRARLAFLAIVTL
                     LGASLLVRGLAAEGDRRLYALVAFAVVAPCVVLAWRDLLRRR"
     gene            complement(40501..42792)
                     /locus_tag="PLP50_01055"
     CDS             complement(40501..42792)
                     /locus_tag="PLP50_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50161.1"
                     /translation="MADPTPRPSAGEPPPVLAVALLGAVIASRFLAIAAAPGEIDEAV
                     FAGAVHRFDIADLSPQAPGFPVWILLGRALLPLAPGAFHALAVAATLLSCLGLPALYL
                     WGRRLVGGWAALGAAVFAAFLPVVWVNGGRAFSDTPSTALFLASLAALGAASARAVSG
                     RPGAVSLAVLAGLAAAAGAGVRPHLVLAFGPLLLVEALLLVRSRSGRPVAAALVASGL
                     AGTAAWLAWLLKQTGGVRGFLAGIDERASFRSGAFETGRFGSILDSFLVRDFLSWPLA
                     LVIALLALLGLGATFRRLPRGAAALLLVLAPAFYSLWFLHSREMSRYSVPFVLVLALP
                     VAAGVEVLVRRPPLGLAAMVAGAALAGRASWGEVRYGATNPTPPSAAIDALSLYGHPG
                     RETIVSDGIFNAFLRLERWEKRLRVWPLSDDILLREGVRRNRRLVRLADFTSEGDSPD
                     RTDPAWRVFRHEGRVARALGNGRLFVVAVRDPAPPLFGPGFGPRESVPGWPSTRWAGP
                     EARLIVPGLQGPPVALLRGVRQHHEGATTLKVRDAETGRTVLERRIEPGDFEVAFLPQ
                     PIYGPLPKPRELVLSCDRPEAVPAAPGLVRPSRGCFLFREATFSLPPEEVWERLGEER
                     LLDLGRPRDAWTDLDGFYDREVHEKSGATWRWTSGRAAFTWVPVPGLAPGELVLRARA
                     PGASGVRVDVAFDGRAAGSVEVAPGDFAEVRIPLDPPARERLRAGDPVRITLATPVLV
                     PRDAGLGGDPRRLGIALDRVLLR"
     gene            42894..44282
                     /locus_tag="PLP50_01060"
     CDS             42894..44282
                     /locus_tag="PLP50_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPA50162.1"
                     /translation="MAEPRIIPRAEHPISRGYIDPDALKVLYRLHRAGYKAYLVGGSV
                     RDLLTGRTPKDFDVGTDARPQDVRRLFRNSRVIGRRFRLAHVLFDGGKLVEVSTFRRR
                     PDPADAEPGPGGDDDLLIRSDNTFGTPAEDAVRRDFTINGLFYDIATFAVIDWVGGVE
                     DLERHVIRTIGDPEIRFREDPVRMMRACEFAARLDFRMTPGVRAAIEENRKEIRKSAS
                     PRVTEELLDPLRRGWGAPTYRLWSETGLLGEIFPALEAEIGGASRGTGLFWKLLAETD
                     RRRGGGEVLSDSILLGVVFLPLLFAAIRARSGGSVDAQKLLLLLEEVVNPEAARLSLP
                     NAAIHDLKQGLFTMGRIAELRPGDPAARRLVTKGYFPVAVGLLSLYSAASGRYRDAAR
                     TWEDLLRRGGRTPRPGEDLRAAIPFPEALIPHEETGPTELLLPDEAPPRGRTAPRRRR
                     RRGRGGRAGEAA"
     gene            complement(44287..44940)
                     /locus_tag="PLP50_01065"
     CDS             complement(44287..44940)
                     /locus_tag="PLP50_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HPA50163.1"
                     /translation="MRRARFALSTLLLAGVPAAGTEPPPRVEEVAALLTGTFDSSAQS
                     GSDPEGYRAVRLVAVRVPASRLGAGAPVLYVEQALLESPDRPYRQRFYRIEETADGGV
                     VSRVFEPKEPIAVSGKWRDPSDLALYGAGDVVERLGCAVRLMRTDDGWRGSTEGKGCP
                     SALAGARWATSEVTLRPGRMESWDRGYDVNGRQVWGARNGPYVFERRSDGPPAASPK"
     gene            complement(44951..45802)
                     /gene="zupT"
                     /locus_tag="PLP50_01070"
     CDS             complement(44951..45802)
                     /gene="zupT"
                     /locus_tag="PLP50_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPA50164.1"
                     /translation="MRLWRRGGVTTAGNVGVAFGLTLAAGLSTGLGSAIAFFARRTNL
                     RLLSVATGFSAGVMLWVSFLEILPKSQAALATAVGARQGAWLSVGAFFLGILLILAID
                     RLVPGDENPHEVRTALELASLGDAMALPAAAKLRRTGTAVAVAIAIHNFPEGMATFLS
                     ALDDPKVGLAIAIAVAFHNIPEGVSVAVPIYYATGRRRAAFGWSFLSGLAEPVGAAVG
                     YLVLRPFLTSGVMGVVFGAVAGVMVFISLDELLPTSRAFGEGHDSLYGLLLGMGAMSL
                     SLLLLMP"
     gene            complement(45881..47698)
                     /gene="typA"
                     /locus_tag="PLP50_01075"
     CDS             complement(45881..47698)
                     /gene="typA"
                     /locus_tag="PLP50_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPA50165.1"
                     /translation="MPELRNVAIIAHVDHGKTTLVDCLLRQSGTFRAGEHIEERVMDS
                     NDLERERGITILAKNTSIRYHGTKINIVDTPGHSDFGGEVERVLKLVDAVLLVVDAAD
                     GPMPQTRFVLRKALELGLRPIVTVNKVDRPEARPHQVIDEVFDLMVDLGATDHQLDFP
                     IVYTSAKQGFARREVDHSDNDVRPLLDAILVEVPEPGGNAEAPLQILVASLDYDNYLG
                     RLAIGRVVNGVVKPGQNVSICRHDGTIAKGRVVKLMVFEGLKRTEVAEAAAGEIVVLS
                     GLEEVTIGETVADADFPVALDPIAVDEPTVSMTFRVNDSPFAGLEGKYVTSRNIWDRL
                     QKELRTNVALRVEPTDVPDAFKVSGRGELHLAILIETMRREGFELGLSRPEVITREID
                     GVTCEPAEQLVVDVPEEFVGTVIEKIGRRRGEMTALVNNGTGRARIEFKVPTRGLFGY
                     RNEFLTDTKGEGILHHVFAGYEPWKGDVETRRTGALICKEGGDTTAYAIERLEERSVF
                     FFGPGVACYPGMIVGENARENDMIVNPCVKKHLTNMRASNSDMAIKLTPPREMPLEAA
                     IEWIEEDELVEVTPRSIRLRKRVLDHSQRKARKPTTEAA"
     gene            complement(47765..48319)
                     /locus_tag="PLP50_01080"
     CDS             complement(47765..48319)
                     /locus_tag="PLP50_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50166.1"
                     /translation="MFSARNVRVPVLLALLPLLLAGASLEAQDPPPAQEWKIFQEFGF
                     LNGSWAGPAESGGRVGGRVVSFTSSVGGAQLSYRATTFYPAKDALPESSTEEVAYIVY
                     DGGKGKYVALVVFSTKVWGIYEADVRPDGIVFTAKEMANLEAGIRSRWTFTRRADGTI
                     GELLEVAPAGKEFAPYVSAVLSRK"
     gene            48416..49045
                     /locus_tag="PLP50_01085"
     CDS             48416..49045
                     /locus_tag="PLP50_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50167.1"
                     /translation="MANAPHPDAELVARCLNGDEGAWRELVERYSAKVYRIAYHRTYD
                     RAEAEELTQDCFLKVWENLDRYEPTEASLLAWIATLSRNLSIDHYRKRRREKGFRFVT
                     DDALVTLLPGSEDPSADAVRRERLRLLLDAIAQLPDELAEIVQMRDLDGLDYREIADL
                     LGLPDGTVKSRLNRARVELARIVRERIGATPLARGPLFGGLTPQAGGAT"
     gene            49042..49845
                     /locus_tag="PLP50_01090"
     CDS             49042..49845
                     /locus_tag="PLP50_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50168.1"
                     /translation="MSGGAAVPCRRAETALLSGGVPVPPEVGAHASSCETCGPLLSGC
                     EEIHALLAALVVPEPPPALSGTLAAIGSTEPPRVAARETLALLSPEALAVPEPSPALL
                     RRLLAVPATGPRRAANVVEMSRFRRLRSDWRVLVAVAYAACLALVAILGVDPLSAARS
                     GASGMAAVGERAIAEAREAAAGKLDTVLAAQRKKPLTEQIDYKVYRTLAVGKAKTSAW
                     AGILLGRIFGTRVEAEAPAAPRRSEPDPGSLRSFDRAGTETPLSRAQRA"
     gene            49869..50453
                     /locus_tag="PLP50_01095"
     CDS             49869..50453
                     /locus_tag="PLP50_01095"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPA50169.1"
                     /translation="MNENPNLPPPPVSAAAAPPVAYAPPAPPRLAAPPGKNPAAAGLL
                     SLFPGLGHVYLGLYKRGVVFFAIWAFFIAAVDRGHDMPFGLLIPFWMVFVLIDAVRQA
                     RALNATGMPEPGFFGEERPFRADGGLTFGIFLILIGLFFLADRIFTIDLSFLLDWWPV
                     LLVAFGGWQVFAYFQEKKRRDEAAASREAGALEP"
     gene            complement(50569..51099)
                     /locus_tag="PLP50_01100"
     CDS             complement(50569..51099)
                     /locus_tag="PLP50_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50170.1"
                     /translation="MAPVLALLTLFWLAEPAPVKGVPAPPGGRFEPEMTKVVSDRAKV
                     EMQSVLAELERSPQPPEAKAEIRRRLAGLRLAVYTTPRGWEEAVAFYEGAPMQLVFLK
                     GERDILGDLREWARSTGVGVDASAEKAWAGKSGRTARWTKDDDTLQVVVEDHLIDPRD
                     GKVSETTVVLVTSLGG"
     gene            complement(51111..52043)
                     /locus_tag="PLP50_01105"
     CDS             complement(51111..52043)
                     /locus_tag="PLP50_01105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPA50171.1"
                     /translation="MTSGRDLFAPKLGLTLPLCGLHFVFFFLFSMDPQKTASLFGFWP
                     QQALSQPWGFVTFQFLHGGPVSLFFGAFALYILGSALEAEWGTAEYTAFWLVATLGAS
                     LSAWLLGTPLVSDPFVVPISMLFAYAVLFPEAQFLVFFVIPVKVKWIAVFSAALLGLA
                     FFRDLVTFGAGPAFVRVLGAGAGFLFFWVRHHGRFKARKAAREAVVAVKTAVVQQKDE
                     TLEKRNRELFPRVDALRATERSGVEPPGGEGLRNELQKLVVPGVKICKPVDFKGDKDL
                     VCLRCEGFAECSLRYLAGQPSEIVVKKRDGGAGG"
     gene            complement(52040..53866)
                     /gene="mutL"
                     /locus_tag="PLP50_01110"
     CDS             complement(52040..53866)
                     /gene="mutL"
                     /locus_tag="PLP50_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPA50172.1"
                     /translation="MARIAVLPDVLVDQIAAGEVVERPASVVKELVENALDAGARRVD
                     VEIEGGGIARISVSDDGCGMDADDARLALERHATSKVRTAEDLGRIGSLGFRGEALPS
                     IASVSRLVLVTSPDGSGLGTEVVADRGETPVARPARQTKGTRVVVEDLFGNVPARRKY
                     LKSQDAEQRAVVKALTTLALSRPDVSFTLRAGERELLSLPSAAGALDRFAEVMGRGSL
                     PRVIAVDSVHAGLRLLGAVSPPETTFASRTFQWLFVNGRAARDGSVAHAARLAAEEAL
                     IGERHPAWALFVTCPPERVDPNVHPQKSEVRFLDPAAVHALVHRGLRAALTEGKSAAE
                     LPGSVLAGGGRGLPGSARAAGWTDALPAAAALAETLGLWDGAALAPGGTASAFPAEPS
                     RGSWEGASSVAAVASPAGTLRLLGQYRESYLVAEGADGLVLVDQHVAHERVRFERIRD
                     CLEGQGLASQALLSPVSFEAAPEEAEALRRADETLSAAGFAVSELAGRLFVVSAAPAD
                     VPADKVVAVLRDLLGRLLEAAGTGAGRAGDTAARMKDALAASLACRGAITVNRRLAPE
                     EAARLLADLSSCRDPWTCPHGRPILLTLAHAEVLKRFGRSSG"
     gene            53912..54667
                     /locus_tag="PLP50_01115"
     CDS             53912..54667
                     /locus_tag="PLP50_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50173.1"
                     /translation="MERSRRLSPALFALAALVLAILVAASVLVGRVARLPREMAGAVP
                     HGIRETAAAVSDGLAKLAAAIRTRSVTTELRSAVAELRGTKKLQVAELTQLEVVERRD
                     ATRLLGVPLPDVVVSARAPVTYVYTLDLDARWDFDLTGRTVSVLAPDLVANAPAVDVS
                     KLELVKTESSILRDEDRVVEELRRSLTDVFRARARQHVPLVRETARKESEAFVRSWLL
                     RAYGLPEDVRVVVRFHGEPATIGPAGVALPPRG"
     gene            complement(54683..55831)
                     /locus_tag="PLP50_01120"
     CDS             complement(54683..55831)
                     /locus_tag="PLP50_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50174.1"
                     /translation="MVPRPAGRDRLEPTTDGTVVLLSLRPKGWSGREEGGPRAPIRPG
                     TAVRWVGDLWEVLLVEPRAGGGWRHVLAPWDERNLVRSVVEYGDAGGPAGSEAAPPEA
                     EPPQPADGAPGIAAPGLPEPAAPGAVEPRPAPPPTKPEARGAQGVAAAWAALAPGVRA
                     LLAAFVPSVLLGWFFPFRVMGEGISFFVHELGHTFVAWFFGCTALPAIVMTVVFEQNR
                     AVAALVWGGLVFLAVRYRRAPRWNLLFAAAALLYPLFAFTKAHVTAFDLGGHLAEAAF
                     AAWAFRRVVRSERPDWERVVWAFFAFYLVARNVKLFGGVALSAAARTDYLTVAITGQN
                     DLVKVAATTGLALATLAALVALLFLVVPLGALVLAFRRPAAEAPVVAD"
     gene            complement(55870..56499)
                     /locus_tag="PLP50_01125"
     CDS             complement(55870..56499)
                     /locus_tag="PLP50_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50175.1"
                     /translation="MTSNRAIALSVLAGACLLVACGKPAEKPAAPEAVPVAEAPAPEA
                     PAAAPAGVTKVTVTSTNGAWPKENAPDFASPRAIAFKDSSIGVASVVVVLTNYDFAPT
                     TMAEYRKRTSPEPGQGRIELSLVRQKQVAPGETLEFVAGMYDPTKYQGQAEMTGSVRI
                     AVAGGTSLSLSQPTGTIEVTALTADSISGKFDVKDKWTQLSGEFHAPLK"
     gene            complement(56654..58102)
                     /locus_tag="PLP50_01130"
     CDS             complement(56654..58102)
                     /locus_tag="PLP50_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPA50176.1"
                     /translation="MTHPLSGLEPKLLWDHFAALAAIPRGSKNEAAASAHVVAVAARL
                     GLPAKVDQVGNVVISKPGTKGLESKPVVVLQGHVDMVCEKNSGTAHDFTKDPIRLIVD
                     GDVVKADGTTLGADNGIGCAAALAILESNDLAHPPLECLFTIDEETGMTGANVLAPGF
                     VTGRRMLNLDTEEEGAVYVGCAGGVDTLATTTVARTKPSAALSPYRISVTGLRGGHSG
                     CDIHEGRVNALKVLARAVTEAGGFGFAAATLDGGSKRNAIAREGFAVGWLDPVKEPEV
                     RAALAKLAEDVRSEAGRFDNPVNVGLERIDAAPDPIAEGDLARIVGFIHAAPHGVLGW
                     SPDIPNLVQTSTNLAIVETKDDVVSFSTSQRSSVESAKKDAGRMMATAMRLAGFEVKQ
                     GDGYPGWKPNVTSPLLGLAKEVHAELFDKEPEVKAIHAGLECGIIGEKYPGMDTISFG
                     PTIRNAHSPDECVSISSVKNFWTYLTAFLPRL"
     gene            58267..60297
                     /locus_tag="PLP50_01135"
     CDS             58267..60297
                     /locus_tag="PLP50_01135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPA50177.1"
                     /translation="MRRPALLALTLLSALPLAGAEPAAKRPLGVDDIWSAVRVGAPVV
                     SRDGTVAVYPRSVWDAEKNRLLSDLWLQPVAGGPGRRLTAHEAGEASPAISPDGARVA
                     FVAKRDGDEVAQLYLLRLDGGEAERLTDLPAAPGSPRFTADGKRIVFSANVLGESLET
                     TKAELARRKKEERTRVLATFSERRLVRYWDRWLEQDEWPHLFSVDVATRAVADLTPGL
                     KRFGRLEGGLDFDVAPDGSVVFAANSTEPPYRTLNTDLFLVPAGGGAPRNLTADKPAE
                     DSSPVFAPDGRTIAYGRETRGDGWPDRTRLALLDVASGKTKALTESWDRVPQGWSFTP
                     DGRTLVFLAEDRARTHVWSVPISGGTPRLVWKGGRATAVETAGPSGLVFTYTTFRRPA
                     ELAAVKLDGSGFRNLTSWNDALVAQIAFGEEREVVFKGAGGDDVQMFVVHPPGFEKGK
                     KYPLVHVVHGGPIGTSGDEFHLRWNAHAFAAPGYVVALVNFHGSSSFGQAFVESILGA
                     PGDKPFQDVMAATDYLVAEGSVDPKRVAAAGGSYGGYLVNWIAGQTDRFAALVSHAGI
                     YSLLGQFGSDLAYGRQHSYGAYPFTNPSAIERWSPNRYAASLKTPMLVVHGEKDFRVP
                     ITQGLELYGVLTAKGVPARLVAYPDENHWVLKGTNAKHWYGEVLGWLARWLK"
     gene            complement(60304..61413)
                     /locus_tag="PLP50_01140"
     CDS             complement(60304..61413)
                     /locus_tag="PLP50_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50178.1"
                     /translation="MILPVLVAAALVGAPALEAAPSPVPVEMDTQDRLDVPIALQLKD
                     ASVVDVLEKFADLLGVTPILDPGVGGRVSVEISNVPLRKALEMIGQAANVEVTVSSKV
                     LRVRAKAGSKATGAATVPAAPASEPVPLGGALRFWLDGADDPPVAVRVPDFVGRVEVP
                     GCAEPLTLGRLGARGGATDGIALAAVDPSGGGAKGRILGEAAASGTRVWLAGCDPRLL
                     VEVGDPLPGAVVKEPLRVPNPEPLVLTMKLLERTEAAEESLSEPRVAFRSDSGFSTKS
                     GFEAGPKDAFGQVAEIHGLPLELRAEDDSLLFAVFAAVTRTPAAGGPALVARRAETFR
                     LKLGKPLRWTIDSSWDGGRAAIVLELLVERMPARR"
     gene            complement(61410..61766)
                     /locus_tag="PLP50_01145"
     CDS             complement(61410..61766)
                     /locus_tag="PLP50_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50179.1"
                     /translation="MSCPDLDHLDDSALSAHLAACPACSSTARRDALLADPLVAAQAS
                     VLRWRRARRTAASGLLAASLVAVALLSVALARRPEPPTVFVLQGDETGVVLTGPDLVR
                     RAESLPPSRPMKGDRL"
     gene            complement(61763..62293)
                     /locus_tag="PLP50_01150"
     CDS             complement(61763..62293)
                     /locus_tag="PLP50_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50180.1"
                     /translation="MSDLPEELCGAPAAPVVDEALRIGSSLRAFLERRGHGEDARDLV
                     QDLFVLLLSRPGRFGAPAPGPLRSLLFAIAWRIGANASRRRRAATEVTTGEELRATND
                     LDPESRALLAERARRAAAALESLPEGTRRALRLVADDGLTPAEAARALGIGEEALRAR
                     LCRGRRRIAAILEEEP"
     gene            complement(62405..62944)
                     /locus_tag="PLP50_01155"
     CDS             complement(62405..62944)
                     /locus_tag="PLP50_01155"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA50181.1"
                     /translation="MKLALLPLAAALLAQPAPAQPGPGVAGAAQKQPGPGIAEAPAVR
                     RPPSPPVYDEAADAKADVAAAVAKAKKEKKRVLVTLGANWCGWCRTLDRLFTKDEAVS
                     AALAASYIPVKVDVGRMTKNLELAKGWGADPKTGVPLLVVLDGTGKAVKVQETGSLES
                     GKRHDPEKVIAFLKANAGV"
     gene            63091..64830
                     /locus_tag="PLP50_01160"
     CDS             63091..64830
                     /locus_tag="PLP50_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50182.1"
                     /translation="MPETQAPRRATARAAALLLSLAALPLAAQPVRNGPLPGPLPLFP
                     PDNWWNVDVSTAPRDPNEAAILAFIGMNRGLHPDFGGDVSASGPDIYGMPYIVVPGSQ
                     PLVPVAFDYDDESDPGAPGRPVGYPIPPEAKTEPKWLEGGWPGSSGAGGDKHLLIVDR
                     DNRLLFETWDTRCVPEGSASCTWRAGSGAVFPLDGNLRRPDGWTSADAAGLAILPGLV
                     RYDEVFGSEPIRHAFRFTVRATNGYVFPASHRAGTTPGAPPFGTRLRLKASRDLSEFP
                     APVRRIFEAMKTYGLILADNGSDMYVQGTYDTRWDNDVLNPAFARLKVSDFEVVQLGW
                     QPAGLPVDPCEAGPGPSCHEWLLASGARVRGDGGTSWKTDLTAVNGGESEATLFLRFL
                     GHDADGRNGPERSFRLAAGRSMTWSDVLGSVFELEEAWGAIQLRSASPDLVVTSQTFT
                     PGGGGSYGQSVPLFAETELIRGSASRTIPAIREDASFRTNLVLANGSEAACDVDVSLV
                     SEGGTLLGTRRVPLPPLGMTQLTRVVRELGVTAAVSGARLVLSTPTASGAFAAYASVV
                     DEKTGDPRTLLPR"
     gene            65106..65510
                     /locus_tag="PLP50_01165"
     CDS             65106..65510
                     /locus_tag="PLP50_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50183.1"
                     /translation="MNGTRKLSALLVGTLFLAGMLALPAAAQQQGPGNGGGAKAGTKR
                     QLGPGDGTGAAPAPKDGSGYGTPYRGENPNAGQGAGPGNGTGNGGNGPKDGTGYGRKA
                     GRGSAGAGSCDGTGPRGGSGGRGSGGRRGGRG"
     gene            65669..66142
                     /locus_tag="PLP50_01170"
     CDS             65669..66142
                     /locus_tag="PLP50_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50184.1"
                     /translation="MPPPPHHHRSQSAVTRFIRPEEIDALLEPWVPDAEDRRFLTRCL
                     VEEGPLHHRGANFVLLRLLGELSRAVASGPERPAPGPGLPVPMRLPPHLRPDGEDDAV
                     YPIRLPERAVERLAPRGSRDFDAIVDCLADGPPQHAVANVLMATILDDLLRRSGR"
     gene            66142..66777
                     /locus_tag="PLP50_01175"
     CDS             66142..66777
                     /locus_tag="PLP50_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50185.1"
                     /translation="MSFFAPGLEDAFRTAAAELGMCSASRLFVREAASDGPDAVAELS
                     DRLGRPFPVLDAVAAAALEGAPLRGVDPGEVLATLAGLRRLLVAGFEADFLDALVRAL
                     SGEVRVGLVRTAFLEGDWERIADNYAGRLELVEMAAFAGWAGPRSGLLTFLYGVEGAV
                     THVAPAWLRVAGDDVRPIFRELVGWDVLGRPMSLYPRWLTEVPAGSFTRLA"
     gene            66774..68099
                     /locus_tag="PLP50_01180"
     CDS             66774..68099
                     /locus_tag="PLP50_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPA50186.1"
                     /translation="MTFAAAATTAILAATVLLQAAWPARRLVLVASGAALSALVVSLS
                     GVSTTGRLLAGVPWDVLVLLVGLGLVTEVLVASRLFGLLAVRVARATRAAPGLLAFVF
                     VLGMYAVSGVVNNLTALMLLLPVVLILFRLMGVSQRYVTWTLATLLVACNLGGAATPI
                     GDFPAILLLGRGAMEFTEYLVRAAPLTLAALTLLLAAVFGLARPARGLAATPLSARLT
                     VATLSAFHRGVRLDRRALLSGLLPLAGMVAAWLLVPASTGVGPELICWIGAAAALLGA
                     GRLGERLARTRVDVEATLFLLFLFVMVGAVGETGVFRDAARLLAALPLPPTAQVAVFL
                     VSSALLTGVFSAGPSMAALLPVAEVLAADHPPEAIYVGLALSVCAGSSLFLTAATAGP
                     LTQALVERAGLRGEDGAPVDLGFFRFAPVGLLGFGVTLAVALVATLARL"
     gene            complement(68056..68562)
                     /locus_tag="PLP50_01185"
     CDS             complement(68056..68562)
                     /locus_tag="PLP50_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPA50187.1"
                     /translation="MGRTTGPVHAREASLGEALDFMKLLWRLDHALQTTSRRMAESVG
                     VTGPQRLALRVVGRFPGITPGETAAVLRLHPSTVTVIVKGLERAGLVERSEDPSDRRR
                     TRLHATAAGRKVSSATRGTVEEAVRRTLARFPAGDLETAAGVLSALSAELEPPTGGRA
                     SRRAPRRG"
     gene            68665..69615
                     /locus_tag="PLP50_01190"
     CDS             68665..69615
                     /locus_tag="PLP50_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPA50188.1"
                     /translation="MTSSRNPARPPSSSRVAVFGEALVDELPSGGRPGGAPLNVAAHA
                     AAFGLSPLLLTRLGSDPAGEALRAAMASRGIPADGVQSDPARPTGRAVVTLRGGAPRF
                     EIPAGAAFDAIDGSAAARVVHEARPSVVAFGTLALREPASREALREVLDASPAIRLVD
                     LNLRAPWYDGAVVTRALSAAHVVKVSDEELDELRRLLCLTLREPEALAADVVRRFRLK
                     ALYVTRGARGAAVSWRSGDVVHRAEADAAGSQDEVVDTVGAGDAFTAVVLLGLVRHWT
                     AEESLRRGVELAGAVTRIAGATPEEPSFYAPFLAQWSEAK"
     gene            69612..71765
                     /locus_tag="PLP50_01195"
     CDS             69612..71765
                     /locus_tag="PLP50_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPA50189.1"
                     /translation="MNGSADGLYLFHLSLHGLLRGTSPELGRDPDTGGQITYVLELVR
                     ALGRSPRVSRVDLVTRLVEDPAVGPDYAVPIEPLGESVRIVRLPFGPKRYLRKELLWP
                     HLPALVDRCVEHLRAQERLPHLIHTHYADAGYVGVRLSQLLGIPLVHTGHSLGRTKRS
                     RYLAAGRAEASLERPFRFSQRIPAEEEVLENARLVLASTRQEVDEQWGAYENFRPGRF
                     AVVPPGVDTSRFSPPSPFRARTSFPEIDRWLREPHRPLVLAISRPSPKKNLPALVEAF
                     GRDPRLRETANLAIFAGVREDVARSDEEAKAVYTELLLAADRHDLWGSIALPKKHSPE
                     EVPDLYRLAARRRGVFVNAALIEPFGLTLLEAAASGLPVVATEDGGPRDILANCRNGL
                     LVDPLDVDALSRALVEALGDAGRWSSWSRNGLRRVRDTYSWDGHVSRYLALCERLLRR
                     DRKALRRRHAALKATPLAHLADATHLLVTDIDDTLIGDAASLARLLEWRRAHAGRVAF
                     GVATGRSFPRTLEVLAKWRVPLPDVLVTSVGTEIRFAPELRLDRAWEGHIRHGWRRDE
                     VVRALAGATSLRKQPEENDGPYKASWDVVPGRNVDLAAIQRLLRARGLAARLVFSQGK
                     FLDVLPVRASKGLAVRFLALRLGLPLESFLVAGDSGNDLEMLVGDTRAVVVGNHKPEL
                     EELRGRDGVYFATAPYAGGILEGIAHYSFATEAPC"
     gene            71759..74149
                     /locus_tag="PLP50_01200"
     CDS             71759..74149
                     /locus_tag="PLP50_01200"
                     /EC_number="2.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016157 - sucrose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005985 - sucrose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose synthase"
                     /protein_id="HPA50190.1"
                     /translation="MLTRVHDFAAAHPDVLAVLVSRWLAEGRDILLRSDLVRGWEETR
                     AVCDRVDLAASPLAEAAAALHEGVFRAPWIALSFRTGPGRWRYARLHADRLVPEEITV
                     SEFLRFKEGIVSYGRGEEPLELDFGPFGREFPRLTEPRSIGQGLSFLNRHLASRLFRD
                     GGSGADRIHTFLGLHALEGRTLLLRASFADTAALRAALRKAHDLLAAAEPATPYRELE
                     ERLAGLGFAPGWGATAGRAAETMSLLIDLLEAPSATLLEAFLARVPMISRVLVLSPHG
                     WFGQSGVLGLPDTGGQVVYILDQVRALEREMRERLAEQGIDVEPRIVVVTRLIPDAGT
                     TGCDERLERIDGCRSAVILRVPFREADGSVVRPFLSRFKVWPYLHRFSHEVETEAAAE
                     LGGRPDLILGNYSDGNLVAALIGMRLGVTHATIAHALEKTKYLLSAVHWKEMEEEYAF
                     SVQYTADLFAMNSADFIVTSTYQEIAGTKEAVGQYESYSSFTMPGLYRVVNGVDVFDP
                     RFNIVSPGADADVYFSFRDRERRLAVLHPAIEELVLGEPGPEARGRLVDPGKPIVFTM
                     ARLDRIKNLAGLVGWWAHDERLRSLANLVVVGGHVDPDRCADGEEHEQARLLHDRMTR
                     HALDGEVRWIGKRLDRHLTGELYRWVADRRGLFVQPALFEAFGLTVVEAMVSGLPVFA
                     TCHGGPSEIIEDSRSGFHVDPNDGAAATARIAAFLERCAADPSAWDRVSEAAAARVAA
                     RYTWRRYAERIMTFSRIYGFWKFVSGLERQETARYLQTLDHLLLRPRAAAVGGGPG"
     gene            74377..75936
                     /locus_tag="PLP50_01205"
     CDS             74377..75936
                     /locus_tag="PLP50_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPA50191.1"
                     /translation="MAIPAAAGALLAYLVPLFAPRAGGGLALVRKAYAGDPTTLLDPR
                     TLVGAFGANSLSLGAGVPLGPEGPTVVLTSGFAAFAARWLRLPGRFVRGMVPVGTAAG
                     IAAIFNAPITGVVFALEEVIGAASRGVLGGSLLAAVAAAVVERTFLGGGRLLPAAPAH
                     WGDLRELLGFAVLGLLCGAAAGWLPRFVPILGRGLEALLVRTGRHALVARGALAGAVA
                     GLLGVVAPETLGIGYPAVSEWLTGGGSGPQAAIAFAAKLAGVSLALAAPLVGGVFAPS
                     LFLGASLGAAAGHLGQHLFPLAHIEPGAWALVGMGAFFAGFLRTPIASVLIVFEVTGD
                     YGLVLPQMLTVVLASVVARRISRETLVERALAPAERRPGGRHDPLATLRVADVMTTDL
                     RSPRASMTILEAARVAAGTRHEQYPLVDDAGLLAGVLFARDLDRAILENRKEATVGSL
                     ALPPVLVLTPEVTLDDAALRLARAGESRAPVVASEEEPRLVGFLSSSDLVRARLKVHE
                     EESGPSMADLG"
     gene            75933..77207
                     /locus_tag="PLP50_01210"
     CDS             75933..77207
                     /locus_tag="PLP50_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA50192.1"
                     /translation="MSRRGAGASAVLALLLAASACRPGGNAAPGDGVVLRMLVPANER
                     PFWLPLAETFAKARPGVRLELEEGPVPTDLRESLTTSALLSRDGAYDLVYLDVTWTSK
                     LAAAGWLLPLDDAFGEEERAALLPQALTAGVFRGRLYRVPFRTDVGLLYYRADLLAKA
                     GIAPPRTFAELEAAARRLQSPPDRWGFLWQGAQYEGLVCFFLEVLHGHGGSWIDPETL
                     EVGLDRPEAEAALAFLVRARGAGGISPPGVTAYREEETRHLFQSGRAVFLRSWPYVFR
                     LAQREESAVAGRIGVLPVPTLSGAPGSGTLGGWGLAVARASRRPEEAIAFVRHATTLP
                     SQRLFCAPTGFAPARRDAWADPELLAANPFLAELGRIHASATARPVVARYAQASDVLQ
                     RHLTAALAGLSPPRAALAAAARETRALLGEAP"
     gene            77204..78094
                     /locus_tag="PLP50_01215"
     CDS             77204..78094
                     /locus_tag="PLP50_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPA50193.1"
                     /translation="MSTVLARRARRERALLLSPVLLLLALVVAVPALRVLLLGFTRTE
                     LADGVVSSWAGLATHARTLGDGRFLSALRNTAVFTFLSVLLEVTLGTALALLLDRSFR
                     GRGVLRAVVLVPWALPTSVMALAWAWIFHDTFGMANDLLLRIGLVETPVAWLAQPGTA
                     MAALVVADAWKTTPFVALIILAGLQGIPREVVEAARVDGAGAFARFRRITLPLLVPSL
                     VVATLFRSIQAWGAFDVVWVMTGGGPGGSTETLSLYAYQSWFRYLDFGYGAAIAVQGM
                     LVALLLAALVVRLASRREEA"
     gene            78091..78924
                     /locus_tag="PLP50_01220"
     CDS             78091..78924
                     /locus_tag="PLP50_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPA50194.1"
                     /translation="MRRSVAAAFLALGVCLAAAWSLLPAGWQALTAVKPEAQITASPA
                     VWVPSPPTTEHVRSLLARKPFDRYLANSALVSLAATLLALAAAAPAASALCRMRPRAR
                     SRWLLGLLLVSLFPPILLLFPLYEGVRLLGLVNNPLALVLPYAAFGVPLATWVLEAGF
                     RELPKEVEEAATLDGLGPLGRLLRVQLPLAAPSVAAAGLLTFIASWNEFLLALSFTTR
                     ESSRTVTAGIASVSGASLYEVPWGPLSAAVVLATLPVVLLVLLFERRISGGLTGGAVK
                     G"
     gene            complement(79025..79966)
                     /locus_tag="PLP50_01225"
     CDS             complement(79025..79966)
                     /locus_tag="PLP50_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50195.1"
                     /translation="MSHRFIATRVAFAGVLAAALALGGCGWKKEIEQLRTDNQKLSQE
                     KQQLEGQLTSAGVESAEMQATLDEVAKSLEELRAKELQIVKSSIEVVQEGKPRSTQRD
                     ELKTEIDAIKKAIQENLQKLARLEQEKKDALAKAKASGAKVVELEGQVTSMERLIGEM
                     KASLEEKTTLITELETKVLGLTQTVEEQTATIAEKETVIEGQTKVINTAHVAIAGQKE
                     LEAKGVIEKKGAFLGMRGSWVQTGKFDPEIFREIDITAEQAFAIDAPLKKVQVISAHP
                     KGSYELFAADETHTTLKIVDAAAFWSGEKFLVVKYKD"
     gene            80143..81351
                     /locus_tag="PLP50_01230"
     CDS             80143..81351
                     /locus_tag="PLP50_01230"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPA50196.1"
                     /translation="MSNVRTKRGTIAILTGGGDVPGLNPAIRAITVRALRDGFRVLGI
                     RRGWAGLIELDGGTGRPDGDCVVELSEEIVNKIGRTGGTFLHTSRTRPSHVPRANVPE
                     HLRDRYSGEMNDLTPEVLSNVGKLGIDVLIPIGGDDTLSYGVRLHKEGVKVVALPKTM
                     DNDVPGTDYCIGFSTCVTRTIEMTHALRTSAGSHERFLVIEVFGRYAGFTALLPTMAG
                     AANRCVIPEHPFDVDRLTELLVADRTTNPSRYSVVLVSEGAKFAGMDEMVYSDQEQDA
                     YGHKKLGGVGDLVAEKLRQLSPRHAGGRRVNTISQRLGYLVRCGDPDALDSIVPMAFG
                     NLAMDLILRGSFGRLVCVRNGRYDDVPVDVVTSRKKSVDVAKHYVADRLRPRYEDFGN
                     KPVFIMTSDY"
     gene            81408..82367
                     /gene="corA"
                     /locus_tag="PLP50_01235"
     CDS             81408..82367
                     /gene="corA"
                     /locus_tag="PLP50_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPA50197.1"
                     /translation="MIRLYGFVDGTLRALHLAPMGEASGPEWDAVAWVDLLEPEPEEK
                     SWVERVWGIEVPSRSEMRELEASSRCYQEADSLHLLTAFIASEPGPAHTIDVWFNLHR
                     GRLFTVRPEKLPPFRLFRLRATSQPEEVREPIDIPLGIIEAEIDVLSDVLEAAYARLE
                     RVSRSVLTEGARDLEDLLEKLAAEEDLQGKTRLVLLDLRRSLTFLLRYVVGSTPRANV
                     IKGALRDIESLTSHSGFVFEKVKFLMDATMGFINLEQNKIIKIFSIAAVVFLPPTLVA
                     SIYGMNFRVLPELEWLWGYPFALGLMVLSGIAPYWLFKRKGWL"
     gene            complement(82372..>83164)
                     /locus_tag="PLP50_01240"
     CDS             complement(82372..>83164)
                     /locus_tag="PLP50_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPA50198.1"
                     /translation="GRFEVRDGDVLANLPFHPSCSMWFDHHELVDANARPEAAFQGRY
                     GLAPSTARLVHDYYCERKGWRLFERYGELVAGTDRVDSANLTRRDVLDPERVILLGFT
                     IDSRTGLSGFRDYFLFLGMALRSMPLDEVLRSPQVTERVAKMREADEEFRKALLASSR
                     LEANVVVTDFRDLSPIPIGNRFLVYTLFPEATVSVRLQWGPRRDVVMATVGHNIFERS
                     SWSDVGHTMSLFGGGGHRGAGSCPLPPDDGDATLRRLVAELKRQG"
BASE COUNT        11054 a        30995 c        29278 g        11837 t
ORIGIN      
        1 cgtggacggc taccacgtcg tgatggacct cgagcgctcg cacgggagct tcatctacga
       61 cagccgccac gacgtcgaga tcctcgacct cttctcccat ttcgccacga tcccggtcgg
      121 ctacaaccac ccgagcctga aggagccctc cttcctcgag gagctccgcc gcgccgcgct
      181 cacgaagccg gcgaacagcg acatctacac cgaggagatg gcccgcttcg tcgagacgtt
      241 cgcccgggtc gcggtcccgc cgagccactc cgagcacctc ttcttcgtcg aagggggctc
      301 cgtgggcgtc gagaacgcgc tcaaggccgc cttcgactgg aaggtgcgga ggaacttccg
      361 gaagggctac cggcgcgagg tcggccagca ggtcgtccac ttcgagcagg ccttccacgg
      421 gaggaccggc tactcgctct ccctgacgaa cacggccgac ccgcggaaga cgatgtactt
      481 cccgaagttc cgctggccgc ggatcgtcaa cccgaagctc cgcttccccg tcgacgcggc
      541 cgagctcgag cgcgtcgaga aggtcgagtc cctcgcgatc gcgcaggtga agcaggccct
      601 cgtcgagaat cgcgacgacc tcgcggcgct gatcatcgag ccgatccagg ccgagggggg
      661 cgacaaccac ttccggaccg agttcctcgc ggcgctccgg cagctctgcg acgagaacga
      721 gatgctcctc gtctgcgacg aggtgcagac gggcgtcggc ctgaccggga gcatgtgggg
      781 cttccagacg atgggcgtcg tccccgacct tttcgcgttc ggcaagaaga tgcaggtctg
      841 cggcttcgcg tcgaacggcc ggatcctgga cgaggccgag aacgtcttca ccgtctcctc
      901 gcggatcaac tccacgtggg gcgggaacct cgtcgacatg gtccgcgccc ggaaatacct
      961 cgagatcatc gaggaggaga agctcgtcga gaacgcccgg gccgtcggga cggtcctcct
     1021 cgaggagctc gccgcgctct ccgccgagtt ccccgggaag gtgacgaacg tccgcgggag
     1081 gggcctcttc atcgcgttcg acctgccgga cggcgcgacg cgcgggaacg tcctctcgac
     1141 gatgctccag aagcaccacg tcatggctct cgcgtgcggg gagagctcga tccgcctccg
     1201 ccccgcgctg acgctcggcc gcgacgaggc cctcctcggc gtgaagcgcc tccgcgccgc
     1261 gctcgccgag gtcctcggct gaggcggtcg acgcgccccg catgacggcc gcccggggag
     1321 cctcgtgccg cgcgaggctc ctcgccctcg gggcggcggc cctcgccgcg ctgctcctcg
     1381 gcgttttcgt cccggtcccg cggatcgtcg cgctccgctt cgggccgccg tcgtcgacgg
     1441 cgttcctcga ggcccgccgc gagcggcttc gcgccgaagg acggaacgcc cggatcgacc
     1501 gccgccccgt tcccctcgag aaggtctccc cgcacctcgt gaccgccgtc ctcgtctccg
     1561 aggacgcccg gttcttcgag catcacggta tcgactggag cgccgtgaag gccgcccgga
     1621 cgcggaaccg gcgtttcccg aagcgccggc cgctcggggc ctcgacgatc acgcagcagc
     1681 tcgccaagaa cctcttcctc tccccttcgc ggtcctggct ccggaagggg cgcgaggcgg
     1741 ccgtcgcgac ggcgatggag ctcctcctgc cgaagcggac gatcctcgag cactacctct
     1801 cgggaatcga gtggggggag cgcgtctggg gctgcgaggc ggccgcccgg acctacttcg
     1861 gccgaccggc gtcggccctg acgcgcgagc aggccgcctg gctcgccgcg atgatccccg
     1921 ggccccgcgt ctacctcgcg cacccgatcc gccatcagcg ccgagccgcc cgaatcctgg
     1981 cccggatgcg ccgcgccggg gacgtccggc cgctgctcct cgaggaggcc gacgatccgc
     2041 ccgcgcggta agatttgagg gcaatgagcc gtagcgttgc agcgtcacgg gttagcctcg
     2101 atcggtggcg ccgcggtggc gccgccccga cttttccaca gtagaaaccg cctcgacggc
     2161 gcctcgaagg tgctccgtct ccaggtggct gtagcgaacc gtctgagaga tgttcgcgtg
     2221 cccgagggtc tccttcacgg agaggagcgg gactcccgac tgcacggccc agctcgcggc
     2281 cgtgtgacgg accgaatgcg ggtgaagcgc cttccggagc ctcgccgcgt cccggactcg
     2341 gtagaagagc cgctgtagct ggcgaccgtc ccagggcgaa ccgtcctccc gggtgaagac
     2401 gggagcggcc gggagcccgc gctgcaacga atcgaggacc tcgcgaagct ctccggcgag
     2461 agggagcgtc ttcactcggc cgcggcgctt cggctgagcg atcgtgacgc gcccgccctc
     2521 gaggtcgaac gatccccagg tcaggcccga aagctctccg atgcggcacg cgaggaggag
     2581 gagcgcccgg aaggccggga ccgcgtcggg ccgcagctct tcgagcttcc gcatgaaagc
     2641 ggcccattcc tcagcccgga aaaagacggt ctccggagac ggcgtcgaga gccggtcgaa
     2701 cgatccgcgc ggaagggggt cgcgctcgat ccatccgagg gcgaggacct tccggacgat
     2761 cgcgagcaga tagacgacct cgccgttcac ggtcgacggc atcacgccag cgtcgacccg
     2821 cgcggcctga tacgcgacga cgtccgacgg gcggaggtcc tcgacgcgcc gatcgccgaa
     2881 gagcgagacc agacggttga cgatgttctc gacgttgacg agtgttcccg gggcgaggcg
     2941 gcccctctga atcccgagat actccgccgc cgcgtcggcg aacgtcaggc gccgcgggat
     3001 cgggcgaccg cgcctcgccg cggccttcgc gacggcggcc cggtaggtcg cctcggcctc
     3061 ggtccgcgtc gttcccttcg ggagcgtgac ttgaacgcga ggcccatcct tccggccggc
     3121 cgtgtagagc cggaaccgcc acctcccatc cgggagctgc cggaggccgg gcgacgcctt
     3181 cacgccgctt ccctctcgac gcggatgctc ggagggagga ggacgaggaa ttcgctctcc
     3241 tctcgctccg tgatgtacgc gagaacgtga cgggtcggga ctacggcccg gtatacggcc
     3301 gggctcttca gtcccatgag ccgcgcccgg gatgcgaacc attcggcgac ggcccggtcc
     3361 gatgtccagg agagcccgcg cacgcggcgc gccggcccgt tacccgcgac gccgcgaaag
     3421 agagtgaacg gcccacccgt cgggagcggc gcgcccgctt cccgaaggcg gcgccggtcc
     3481 gctctcgcga acaggccgcg gaggcgcgcc gctgggacgt gaaggcaatt cgtcctcggc
     3541 gcctgatagg cgtcgagcag cgctgcctcg aagatcccga gggactcgaa gaggactcgg
     3601 ttccgccata gaagatcaag gctcaggagg ttgtccgcct tcaagaaaaa gccgacgatg
     3661 ttcctctcgg agagggcgtc gatcgctcgc tcccggagca cgtcgggaat gccggtcagg
     3721 tccgcccgtg cggcgatctc ctcgcgcgag aggccgggcg acgccttcac ttccggaccc
     3781 gctcccggtg cgcggtcgcg accgcgtcga ggacccggac ggcgaagccg gagagcgtga
     3841 gcccgtaatg ctcggccgcg gcccggatat cgtccttctg ctgcggcgtg aggtagacgg
     3901 tcaggcgctc actcggacgg aggaggaggt cgacgcgctc cggcgggagt ccacgtagaa
     3961 gctcggcgag ctggtcgagc ttctcggaga cctcgcgcga catcacgcgg cctcccgcag
     4021 tttggcggcg cggcggagga tcatgaggac gacgtttgac ggccatacgt gggcgccatt
     4081 ctgccgcttg atctgtcgcc tctccagctc ttccgcgatc gcccggaggg aaaggccggc
     4141 ctcgcgaagg tcgatcatga gccggatagc cgtctgctct tcctcgacgg cgacgagctg
     4201 cccggcctcg agccggtagc cgtaagggat cttcccggag atccgctcgc cctgccggcg
     4261 cttgaatgcg agcgccgacg tcgtccgttc ggagacgagg tcacgctcaa actcggcgag
     4321 gacggcgagc atcctgaaga ccatcttccc ggcggccgag gtcgtgtcga tcgactcgga
     4381 gagggagacg aggtcggcac cggcccggtc caggcgctcc gcgatcgcga gggcatcctt
     4441 cgtcgagcgc gccatcctcg agaggctgta gacgacgagc ggcgcccggc gcttgcaggc
     4501 gtcggcgagg gccgcttgca ggcccggccg gttcgcggcc ttcccgccgc tgaggccggc
     4561 gtcaacgtga acggccgcga cgtcgaggcc ctgagcgacg gcccaggcgc ggatacgggc
     4621 ctcctgagcg tcgaggctca cgccctccga cgcctgacgc tccgtgctga cccggacgta
     4681 gccgaccgct gactttgtgg cgctcttcat ggcggtaaat atggcgccat ggtagcggcg
     4741 tgtcaagtgt aaccgtgcga ataggccgca cggccgctca tgaccgaccg gtcagtagca
     4801 ggcgggcgcg gcgtaagtcg ggcgggaggt ccggaatccg caagattccg caaagttcgg
     4861 actcacccga cgccagagga agccgggaat tggcgtccgc cgcgtccgcc acgtccgcaa
     4921 cgcgacaacc atgcgggtct aagggcggac gcgagggcgg acgggacgcc agggcggacg
     4981 cgtccgccgg atcgcccccg gcgtccgccg gagccgggat gcccggattc cgcaagattc
     5041 cgcaagatcc cggagcctcg gacgccatcc gtccgggccg gcgtggtagc cgatctccgg
     5101 gtcaacggcc ttgagcgtct cgacggcggc ggcgaacttg ccggccttct cgatcgtgtc
     5161 gtgcccgacg ccgtacgctt tcgccagccg gtagctcgtc ttctccgcct gcaagtcctg
     5221 tttttcagga cttgccttgc gggtgccgcc gatgtccttc ttcgtccggt tgtagtgccg
     5281 ccccatcacc aacgccagtt ggtccggcgt caggttccgc cggtcgcgct cctcgccggg
     5341 ccggaagggc gtcccgtact acgggctccc ttcggcggga tggtgggcgt acggcgccct
     5401 ccatcactcc tcccgcgcgg acgggccgat ccagcccgga gaaccgcccg ccccggccgg
     5461 aggtccgtaa ccagcggccc tccgcgacgt cgaccgaagg ggtggcagtc gacgccgatg
     5521 gagccggggg cgggcggcga gtgaagcatc aggcggtcgc gtgcgcggcg cagtaggggg
     5581 cgtgcccgta gacccgcgtt cccttacagc ggagggtcgt cgtcccgagc tcgttcccgg
     5641 ggtcgccggg cgggatctgc atgaggcagc gtagcgggtc gttcgggttc tccctcgcgt
     5701 gctcgaggca gtagccgccc atcccggagt gagcctcgtt cgggcagtag tggggcgagg
     5761 acgtgtccat gtcgaagcgg aagacgcatc cggacatcgg aagctccttt cagcgtgcgt
     5821 gcgcagcgca gaagccgagg tcgttgacgg gcttgttcgg gcagttcgtc gagatcgcgc
     5881 cgggtgaggc cggcgcgagg atggcgcggc agacctgccg gtcggtccag ttgatgaccc
     5941 tccgcggccg gacgttctgg ccccgcgtcg cgacgtcgta accgtcggcg aggacctctc
     6001 ggtagggggc gcccatcacg cgcccccctt cgacggccag atcggaacgt cgatggggaa
     6061 ccgctcgcgg ccctcggcgt gcccgagatc gtcgctcagg acgttctccg cggtcgggat
     6121 gccgacctgg gcggtttcgc ggtcgcccgc cgcgcggagc gccatgaaga tgccgttcat
     6181 ctcggagcgg tagccctcga ggcgaccgag tgcctccgag aatgccttcc ctgccgccag
     6241 gtagtcgcgg aagacgtcgg cgtagcgctc cctgccgtcg gcgtagcgcc ggcggaggtc
     6301 gccgagccgc tcggcctcgc gggcctcctc gagcttcgcg cggacgagct cggcggcgcg
     6361 ctgggccgcg gcgaggtcgc gctcggcgtc ggccgccttc ttctcggcgg cgtcgcgctt
     6421 cttccgccag tcgtcgacgg ggaggccctc ggcgaggtcc tcgacgaggc ggtcctgggc
     6481 ctcggcctcg gcggcgcggg cctcctcggc gcgcttcgcc agggccgcgg cttccgtctc
     6541 gaggttcgcg aggcgggcgg cgatcttctc ggcgccggac ttgaagaggc tggcgatcat
     6601 cgcgcgtcct ccgtcgtcag ggcgagggcg gcctcggcgt aggtgcatcc gaccgcctcg
     6661 gacgtcgcgc gtgtgaggtc gtccaggggg agcgacgtca ggccgacgcc gaggccgcgg
     6721 acggccgcga gggcgatccg cttccgctcg gcggggtcgg ccgagagcgc gtgcgcctcc
     6781 tgggcggcgc gtctcacctt ctcggcgggc gtcgggttcg cggcggcgag ctcctcgcgg
     6841 acgatttcgc gggtcacgcg gcggaccgga tcggcgaggg ccgcgcgctc ccggcggatc
     6901 tcgtcgcgga tgtcggcccg gagcggggtc gcggcgtccc tcgaggaagc tgctccggcc
     6961 ggagggcggc cgggcgagaa gaaggtcatc ggagcgttag acatcgggat ctcctttcgg
     7021 ttcgcggcgg tcgtcccgcc agtcgttcac gtatcgaact tcgatcgctc cgggctgccc
     7081 tcgcttcgcc tcggcgacga cgtcgagcgc gacacgccgg agcgcttcct gtcggttcgg
     7141 gtcctcggcg tacttcttct cgaggtcggc gaagaaccgc gcgagctgcg gcgagaggac
     7201 gtagtgcggc accggggtct cggcgtggac ggcgtagagc tcggaaagcg tctcgacgat
     7261 ccgaggcggc ttcgctccgc tggccagcag cgccgcggcg aggctcaggg aatcgtggtc
     7321 ggccatcttc agttctcctc atacccggcc tcgcgagaga gccggtttgc ttctgcaagt
     7381 agcgtctcga ccggaacgcc gacgatctga gcgagcttcg cggcgcgcgt cttgaactcg
     7441 gcctcgacaa cctgcttcgg ctcgccgaat gcgtacccga ggatcttcgt caggacggga
     7501 gccgggccgt cgccgtcgat ctcgcggtcc accttcgcca ccagacgggc gcggcggtcc
     7561 tcgtccgcga ggacctcgcg gcaccaggcg gcgaaccgga tgccgggata ccgcggcttc
     7621 cggagggcgc cggcccggtt gcccggcgcg aacttcccgg acgggtcgcg gccgtcgctc
     7681 atcgccgctc accgccgcta tgtgcggccc ccgagggcct cgggcagggc ctccacgcaa
     7741 cgggcctctg agggcctcca ggcgcgattc tggcggtcgt ccggacgcgg tcggcgcgac
     7801 cgagggcgac gcgaaaagcg aaggtagagg gtggtctcat cggttcacct ccagaatttc
     7861 ggatcgggtt ccggtagcaa cggagcggtt gcgagcgggc gggaacgcga gccatccgca
     7921 tctcgagcag acttgccagc ggcggaaggg cttcatccct tcgggcggac acggcacgtc
     7981 ccgtccgttg cagggcggcg gcggcgggcg acgagggaac cggaggagcc tcatgcgagc
     8041 ctcccggccg cgagcgcggc gagtgtggcg aggagccgga ggagagcgcg ggcgatcctc
     8101 atcggcgcgc cctccggcga gggcgacccg agaggacgag cgcggccgtc ttcaggttcg
     8161 ggaagacgag gaggtcgcgg tgccgcacgg cccggcccga cgagacgaga gcgtcgacga
     8221 ggtcgccgaa cgcggcgagg ctgaggccgc tcgcctccca ggccgtcgag acggagatga
     8281 tgagcgggtt catgccgcca cctccgccgc gggccggccg aacgggccgt atgcgtgctc
     8341 ccggcacgcc ggtgctccgg gatgcagcgg gcgcccgcac cggacgaggg agcccggctc
     8401 cggagcctcc tcgtcctcga cgacggggca ggtcccgtcc cgccagcgct cccgcgggag
     8461 gccgtcggcg tcatacgtga aatcggcgtg ctctgactga cccgccacag gcgccacggc
     8521 gccacaaccc gcacggttgt cactcggaat cgtggcgggt gacccgccac aggacccgcc
     8581 acacgattcg gcctcgggag agggcgtcgg tttccgtggc gggtagtgtg gcgggtaggg
     8641 cgccacactt tgacccgcgt ggttgtcgct ttgtggcggc gtggcgggtg tggcggattc
     8701 ctcagactga ggagactccg agagataccg ctcgaaggcg tcggcgaagg tcgggaccgc
     8761 gtatccgcga accgtctcag cgccttcccg gattcgcatc ggccggatgc cgtagggccg
     8821 gaggaggcgg gcgaggtcga gagcgtcgac ctcctcccgg cgcggcccga acttcttcca
     8881 gcgggactcc gggagcgccc gcagctcttc gaggagcttc tcggtccgga tcgtcccgtc
     8941 cgtcctgacg agaagggcga ggacccgccg gacgtcgccg aggagctgca cgccgtctga
     9001 ctcgtcctcc tcggctcgct ctcccgcgag ggcgacgatc gcccggcggg cgcgctcagg
     9061 ccaaggcccg ccggcaaggt ccgcgagggc gaggagcggt tccgctccgt cggccgcccg
     9121 gtctcccagc tcgcgaggga gcgagggccg ggccgtcttg agcttcccga ggttcgccgt
     9181 cgtccacgcc tccaggcggg cgcggacctc gcgggcgtcc ttctcgacgt cccggagccg
     9241 gaaccgttcg gccgaatcgg tccggcgctt ccgcttcatc tcgacgatga aggaccgatc
     9301 cgcgaccgtc tccggcaacg tcccgatacc ggcaagcgcc ttcgggcagt agacggagaa
     9361 atcgacgagc ttgtctgact tcgcgtcgag gactcgcggg acggtcacgc ctcgccggct
     9421 cccggcgttc aagagggcgc gaaccgcttc catgcgggag tctccacgct gcgaaaagag
     9481 ggcgtccgtc tcgtcgagga ggagggtcgg ccggtccttc tcgatcttcc ggtacaggac
     9541 cgcctccgac ggttggacgg cgtgccacgg cgcacgggtg acgacgccga ggacctcgag
     9601 gaggcgcgtc ttaccgctcc tcttctcggc gctcagaatc gcgaggtagg gcgtctggtc
     9661 tgacgcctcg aaggcccagg tatgagccgt ccagagggcg acgagctgcg cttgctcggg
     9721 cgtgacgacg acgaaccggc ggacaaaccg ggtgagggag tcgaggacgt cgacgccgtc
     9781 gacccgggcg agagcgtcgg ccgtcggcgc gtcggccgcg agggactcca gctcctcccg
     9841 cgtcccgccg gccgcgagcc agtcgctcgc gtccttcacg ccgggtcggc ccaggttgag
     9901 cacgcgcacc tcggcggcga cgcctcggag gctctgagcg acggcgcggc cgtgctcccg
     9961 gcccggctca tcgtcgtcag ggacgacgac gacgcgggcg ccgcggagcg tctcgacgta
    10021 gtcagcggcc cgccaactct tcgcgcctcc ggcgtgcgtc gtcgcccgga gcccggcggc
    10081 gcggaggttg tcgacgtcct tttccccctc gacgaggaag agagggagcc cggcccggac
    10141 cgcttcggcg acctcgggca ggtggtagag gacgcgccgc gtcccgttca gattccaggc
    10201 gtagccgtcc ttcccggccg ggtccgggcg ccgctggcgg aagtccttcg gctcgaagcg
    10261 gacgacctcg aagaggagcg cgcccgtctc gtcggtgtag cggtagacgg ccttgatgcg
    10321 cttcgacccg cggcgctcgt cccgctccgg cgagatgtcc gcgaccgtca ggccgagggc
    10381 gccgaggatc gcctcgaggt cgcatccggc gtgacacttc acgagagcgc ggccgtcccg
    10441 gccctgcgag acggagaggc tcgcgctccg gtcctcgtgc gccgggcatt gccacgcgga
    10501 gcccttgccg cgcgagccgt gagcctcgag ggcggccgtg agccgctcgc tcatggcgct
    10561 catcgccggg cctcccaggt ctccgacgcc gcgagggcgg gcgcgttcat gccgtcacct
    10621 cgtcgcaaag gtcggaggcc cattggtcga tcgcatccga gaggcccgcg acggaccgcg
    10681 aggaacggac cgagtcagcc gcgaaccaga gcgcggaaac ggcccaggcc gggacctcgg
    10741 ccttcctcca gtccggaggg ccataggccc tatgtctcgt gacatacccg ttcagaaggt
    10801 gaagtgcatc tacggcaacg ggagggacgg aggcccaggc cgcgccttcc gacgcctgag
    10861 gccgtaggac gctgtagatg atctgcgggt aagcctcgac ggcctcctcc gtgaagtcgc
    10921 cgacgaggtc ggagtgatcc tcgggataga tgtcgcggag cctgaggagc tggcggagat
    10981 gtccctcggt caacgtcccg gacgagagga agacggtcag gaagtccggc agggtgagga
    11041 gtcggagctt ctgcgcgacg tagctctggg tcttcccgat gaggcggccg atctcactct
    11101 gggtcttccc gacgtcctga aggcgacgga atgcctcggc ctcctcgatc ggcgtcaagt
    11161 tctccctgac gacgttctcg acgagtgcca gctcgaaagc ctcgcgctcc gtcacatcgc
    11221 ggacgacggc cggaatctcg gccaggccgg cgagacgggc cgcccgcaag cgccgctcgc
    11281 ccgcaatgat ctggaacctc tctccgacgg gacggagaac tacaggttct agaacgccgt
    11341 gctcccggat cgagccggcc agctctgcga tgccggcgaa gagtcggcgc ggttgtccgg
    11401 ggagagcgtc gatcctgtcg acggcgacga ggaggccggg cgcgttcaag cggcctccct
    11461 cacgctcgcg aggaagcggg cgacctcgtc ggcgggagcg ttccggcgga ccgtcatccc
    11521 gtcaggcgtc gccaggagga ggatcgtctc cccgttcagc aggaacgccg cggctccgtc
    11581 gccgggctcc gcgtcgacgt gccgcccggc ctcgaggacc tcgcggagga ccgggtcggc
    11641 gccggcctcg aggaggacgt cggagagccg ccgccacggc acgggggcgt tcaccgcgtc
    11701 tccttcggcc agacggccgg gtcgttcatg acgagggaca tgagggcgaa ttcgagagcc
    11761 gaggtcggat gaacgtccag ttcccgcgcg attgcctcgg cgagccgctc gattcggccc
    11821 gccgcgcggt agaggttcac gcctcccggc cgctcatgct cggcccaaca gggcgagtcg
    11881 ccgaggaagc gggagagttc acgctcccag gcgatccgct ccgccggctt ccgggccggc
    11941 gctccgaggg acttacgccc cggaggcgtg tttgcctccg tcccgtggga tacgggagtg
    12001 ttatccttcg tcttgcttcg gttgtcccct tcgccctgcc gtcgagcgcc cccgccaggg
    12061 cgctcttcgc ttttacggtg cggcatcgca tcgctccttt ctcacgcggc cggcggaaga
    12121 tccgcggccc atcggcgcag ctcgtcaacc gggatgagga cggcgcggcc cattcggcgc
    12181 gcctccagcc ggccgagtcg gatctcggat cgaagcgcgg cctccgacac tccgacggac
    12241 gcggcggcgt cgcggagccg ggccatgagc ttctggtcga atcgttcgcg ggttcgggga
    12301 atcgccatcg gttcgcctcc tcttcgcgtc ggccgcttga tgcgtgctga gcgcagaggg
    12361 agtgaaccgc ggcgcctcgg ggggcgtcga atccacacgc ctaaggcgtg tcggttctct
    12421 ttcggcctgt ccggtggcga ttcttccgac agcccttagg cttgtcgttt cttcggtggc
    12481 ctcggcatga tctcgggcca ggcagcatcc gggaacgcct cggccgcccg accgatgcga
    12541 agttggagcg cgtcgacgct caggccggcg ccgctcgcga gtttcgctcg cgccgtcgcc
    12601 acggcggaga cgttcggctt cccatcgctc cgggcgaacg tcgccggctt gctccgggcg
    12661 agccgctctg cttcggtctg gacggcttgc gcgagggaga ggacctctcg cgcgcgggaa
    12721 cgccgatcca tctccacggt cgaggcgtgc ttcgcgaaga gggcttgcgt gacgccgagg
    12781 agaagcttct tcgcctctgc gaaggttcgc gatccgtccc tcatgtcgtc cgggagcgag
    12841 ctgagcacgg tcgaggctct gacgatcgcc gcgaggacgc tcggcgcgta gaggccgagc
    12901 gctccgaact cgcggaggta cggaagcacg gcgccgcggg cggagctccg gagaaggtgc
    12961 tccgccttca tcgcgcgttc gagcagctcc gtcggcgagc tgcccaggtc cttcaggatc
    13021 tcggccagct cgaggacctc ggccctctcg gccgccgaga gcgtcgttcc cgccttcgtc
    13081 ggccgggcct tcgtcgtctt caccatgtcg cctccacgcc tcccgggaaa ggccccggcg
    13141 ggaagcgcga ggcgtgcgcg tgtcggcccg gggatcagcc gggcctatcc cgccgaggac
    13201 gagtttagag gtcaggagcg ggctccgagg agagacgaga cggcccgtag tttctccgcg
    13261 cgctcacggg ccgacgcggt ccgcttcgga gcacggccgg ccatctgcgc ctcgagcctc
    13321 tcgtgaaggg ccgcggcaag ctggtcgacg tcccatccgt cgacgacgtc gcggagcgag
    13381 atccgggtca acgcggagtc ttcgtgcgac gcggctccct gccagcggga ccaggagccg
    13441 gaccagtcga ggcgcgcgaa cgtcccgtct tcgaggagga cgaggtccgt cccttcgatc
    13501 ccgccgtcgt tcgcgcgcgg gtgatcccgc tccgggccgg agccggcgag ccggacgccc
    13561 cgctcgtcgt ggtaatcctt ctcggtcgcg gtgctgacgt tgtccggcca gaaggtccgc
    13621 tccgacgcca ggagccgcga ggagagcgct cgaagcgccg ggcggaccgc ctcgacgagc
    13681 tgcccgagga ggcgctcctc ggcggcgcgc tcctcttcca cggcggccgt catctcgtcg
    13741 tgctgcgcgg cgacgttcgc ggcgaggttc gcgagggttt cgggaatgct ggtttcggtc
    13801 atcgcagagt cctcctctgt ccctcgctct tcgcgagggc gttccggtgg cgcccggtgg
    13861 cgccgttcgt tctttcggtg cgattcctgc gcgtcacgac gggaccgaaa ctcgctttct
    13921 gcggcctatg cggacggctc gcggatggcc taagatttga gggcaatgag ccgtcacccc
    13981 cgcctcctcg ccggcgcggc gctcctcctc gcccccctcc tgctcgcctc ctgcggaccg
    14041 aaggcgtccg agatccgcct gaaggagaag aagctgacgg tctacgggct cgaccggacc
    14101 gtctccgtcg gcggcgacgt cgtcgacggg aagggggaga tcgtccccgg cttcatggtc
    14161 gcctgggaga gctcgaaccc gaaagtcgcc accgtcaacc cgcagacggg gtccgtgaag
    14221 tcggtctcgg ccgggaaggc gaggctgacc gcacggctcg acaagccgcc cctcgaggcg
    14281 cacgtcgcgc tcgaggtgat cgacatcgcc tcggtgaacg tctctcctct gcggatgacg
    14341 ctcgcgggcc ccgccgggac gtccttccct ctcgtcctcg agacgagggc gagccgcggg
    14401 gccctcgttc cgatgacggc ggtgtgggtc tcctccgacc cgaaggtggc gaccgtcgac
    14461 gcctcgggga tcgtcacctc cgtcgcggag gggaagacga cggtgaaggc ctcgatcggg
    14521 gacgtctccg gagcgaccga cgtcgtcgtc gtcctccggt cgatcgagac cctggaggcg
    14581 acgccgctga acatcccgct gaaggtcggc gaggcgctcg acgtcacgct cgtcgcccgc
    14641 gacgcgcagg gtcagccgat ccccgacgtg gcggtgacgt ggacgtcgtc cgacccttcg
    14701 gtcgcgacct gctccagcgg gagggtcgtc ggcgtcggac ccggctcgac caccgtgcgc
    14761 gcggtctgcg gcgcgagatc cgcagaggtc gcggtgatcg tgttttgacc gaagccgtcc
    14821 gcctccccgc ggtcgccgga ctcttctacg agggcggcgc gcgggcgctg cgggcggacg
    14881 tcgaacgcta tctggcaggc gcggcctccc cgccccgacc cgtcgcggcc tggcgggcgc
    14941 tcctgctgcc gcacgccggc cacgtctact cgggcgcgat ctgcggcgcc gggctcgcgg
    15001 cggtcgaggt cccgtcgacg gtcctcctcc tcgggcccaa ccaccacgga gcggggaccg
    15061 gcgtcgccct ctcgggggcc ggcgcctggc ggacgcctct cggcgacgtc cccctcgcgg
    15121 gaggcctcgc ggaggagctc cggaacgcgt cccgcgcgat ccggacggac gaggcggccc
    15181 atcgccgcga gcacgcgctg gaggtgatcc tcccgtttct ccaggtcgcg cggcccggac
    15241 tctccatcgc ctgtctcacc ctcggcgagc cggacctctc cgtctgcctc gaggtcggcc
    15301 gggccgtcgc ggaggccgtg acgcgcgtcg aggagcaagg agagcgggtc gggatcgtcg
    15361 tctcgtccga cctcaaccac tacctgcccc gcgagaggaa ccgcgaaaag gacgaccgcg
    15421 cgatcgacgc gcttctcctc ggcgacccgt cggagctctt ctcgcgggtc ctgctgcggg
    15481 agcggatctc catgtgcggc atcctgccgg cgacggctct gctcgcggcc ctccggcacc
    15541 tgccgcccac ccggccccgc ctcgtggcgc gaggcgactc aggagacacc tccggcgaca
    15601 cgagccgcgt cgtcggctac gccgccctcc tctgggaaac ccgcacggga acgaacggat
    15661 cggaaaggaa ggagtgctga tgcgcgttgc gaacttctcg gccgaggtct cgcctttcgc
    15721 caagacgggc ggcctcggcg acgtcgtctc ttctctcccc aaggccctcg ccgcgaccgg
    15781 ccacgacgtg gacgtctggg tgccgttctt cctcgaggcc gccgagtggt tccggaggcg
    15841 cgggacctgg cccgagcagg aggtcgagcc gttcgacgtc tcgatcctcg gcgcgaccta
    15901 cagggtcggc gtgctccgcg cgacgcttcc cggcagcagc gtcccggtcc acttcgtggc
    15961 gcacgacccg ctcttccacc ggcccggcgg gctctacgcg aagaacgacc agggcgtcga
    16021 cgacgggatc tggcgcttct gcctcttcgt ccgcgcggcg atcgaggcgc tgaagcggaa
    16081 gggggaccgg cccgaggtga tccacgcgca cgactggcat ccggggctcg ccccgatgct
    16141 cggcgcctgg agctcctggc gagacccgtg gttcgacgag gtcgcctcgg tcctgacgat
    16201 ccacaacgtc ggctaccagg ggatctacgg tcccgaccag gttccggcgc tcggtcttcc
    16261 gcccgaggcg tggagcggcg ggctcctcga gcactccggc tcgctgaaca tgctgaaggg
    16321 cgcgatcgtc gcggccgaca tggtgacgac ggtctcgcca acgtacgcct gggaggtccg
    16381 gacgccggaa gggggcgccg gcctcgacgg tgtcctccgc ctgaaggcgg cgcggctcac
    16441 cgggatcctg aacggcatcg accgacagga gtggaacccg tccgtcgacc cgcacctggc
    16501 ctcgcgctac tcggccgagg acctgcgcgg gaagctcgac tgccgcggcg acctctgccg
    16561 cctcgcgggc ttcgaggcgg gcgacccggc gatgatcgtc ggctccgtcg gccgtctcgt
    16621 cccgcagaag ggatacgacc tcctcctcga ggccgcgccc gagctcctcc ggcgcggcgt
    16681 ccgcctcgtc ctcctcggct cgggagagcc ggcgctcgaa ggctcgatgc ggttgctcga
    16741 ggcgcactac ccaggccgct tcaaggggtt cgtcggctac gacgaggcgc tctcgcacaa
    16801 gatccaggcg ggcagcgacg ggctcgtgat gccgtcgcgc tacgagccgt gcggcctcac
    16861 gcagatgtac gccctcgcct acggcaccgt gccgatcgtg aggaggaccg gcgggctcgc
    16921 cgactccgtc atcggactcc atggcgagaa cctcgaatgg gcgaccggct tccacttcga
    16981 cgatccctct ccgcacgccg tcgccgccga ggtgctccac gcgcagagcg tcttcttccg
    17041 tcgggaggcc tggtcgcggc tcgtccggaa cgggatgaag gccgacttct cctggggccg
    17101 ctccgcgaac gactacgagc tcgtctaccg gcgcgcgcgg gaggtgcgcg ggctaccctg
    17161 gtaagggctc cggcaaccgg gtcccgttcc aggggaggcg cgaaggcgcc gcggggaggc
    17221 tcgtggcgag cctccccgat ccaccctaga gctgctcgtc ccggatcccg agacccgcca
    17281 cgagccgggc gagcgccgcg tggacccccg gacccgctgc gaggatgttg ccgcgctccc
    17341 gccacgcgcc gccgccctcg aagtcggtca cgagcccgcc cgcctccgaa acgaggacgg
    17401 cccccgccgc ggagtcccag ggagagagcc ccagctcgaa gaagccgtcg aagaccccgc
    17461 aggagacgta ggcgaggtcg agcgccgcgc tcccggcgcg ccggatcgac tgcgcctcga
    17521 ggaagaccgc cttgaagacc cggaggtacg tgtcgatgcg ggactggacg cggaacggga
    17581 agccggtcgc caggaaggcc cccgcgagcc cgtcccgcgg cgacacgccg acgcgccggc
    17641 cgttccggaa ggcgcccgag cccttctcgg cgacgaagag gtcccccttc accgggtccc
    17701 agacgacgcc gaggacgagc tctccgtcgc gcccgccggc cgtccggccg atcgaaacgg
    17761 cccagtgcgg gaagccgcgg acgaagttcg cggtgccgtc cagggggtcg acgatccagg
    17821 cgggagcgcc gccgttcgag cccgtcacgc ccccctcctc tccgatgaag aggtcgccgg
    17881 gaaaggcgcc gaggaggagc tccttcacga gcgcctcgct ctcgcggtcc gcgcgagaga
    17941 cgacgtcgtt ccgccccttc tcctcgacct ccgaggggtc gagggatccg aagtgcccga
    18001 ggaggaggtc tcccgcgcgg cgggcggcct cgacggcggc gtcgagcggg cgggagaccg
    18061 gcatcagtgc cgctggatga cgctgtcgag gatcatcagg aaagcgctct gctgctgggc
    18121 ctcggtcttg ttgatgacgg cgagacacgc tccctgcggg aagagccggt ggagctcatc
    18181 ggcgtcgtgg cccgagacga tgacgatcct cttctcgctg accccgcgct ccttcgcgag
    18241 gcgcgcgacg gagcggccgt cgaggaccgg catctcgagg tcgacgagga ggaggtcgac
    18301 gtccccgccg aggtgcgaca gcgcctccac cgggtcctgg tacgcctcga agacgacctt
    18361 cccgccgtag cgttccgcga agattgcgcg ccaggacgcg gcgtaggcgg acgagtcgtc
    18421 gacgaggacg acgcgcttcg cccgggacgg gggcacggcg ggaggatcgg tcgcggccac
    18481 ggcggagacg gctccgggaa cctgcggcgc cggggccggc gtcagccggc cgcctcgggg
    18541 gcggcggccg ccccgaaccg gtggacctcg cgtcccgaga cgaacgtccg ttcgaccctt
    18601 ccgcggagcg tcttccccgc ccacggcgag ttcgccgaca gcgaccggaa cgacgagaac
    18661 gtcgtcgtgc gcgcggtcga gaagaggacg acgtcggcgg gaaccccttc cgcgagcgtc
    18721 ccgtagggga ggccgaagag gcgcgccggg gcggtggaca tcgcctcgac gaggcgcgag
    18781 agcgagagga gcctcggggc gacgagggcg tcgacgagga cgccgagcgc cgtctccagg
    18841 ccggtgacgc cgaacggcgc ggacgcgaac tccatcgcct tctcgtcggc gtggtgcggg
    18901 gcgtggtccg tcgcaacgca gtcgatcgtc ccgtcggcga gggccgacac gagcgcctgc
    18961 cggtcggcct cgtcccggag cggggggttc atcttgtagc gggtgtcgta gccggagcgg
    19021 gcgagatcct cgtcggtcag cgtcaggtgg tggggcgtca cctcgcagct gacggggacg
    19081 cgcgcggcgc gcgcgcggcg gatcgccgcg aggctgtccc tcacggagac gtgcgcgacg
    19141 tggaggcgcg ctcccgtgag cgcggcgagc tcgacgtcgc gcgcgaccat cgtcgactcg
    19201 gcgacggcgg gccagccggg gagcccgagg cgggtcgagg cccatccctc gtgcatctgg
    19261 ccgcccccga cgaggttcgg gtcctcctcg tggacgacga ccggaaggtc gaacatcgag
    19321 gcgtactcga gggcgcgccg catcaggtcc ccgttcacga ccggcctgcc gtcgtcggag
    19381 atggccacga tcccttcggc cttcatcagg ccgatctcgg cgagcgtgac gcccttctgg
    19441 cccttcgaga cggcgccgat cggcaggacg cggacgcgcg ccgtctcgcg gatccgcttc
    19501 tggacgaagg cggccgtcgg cgcgtcgtcg accacggggt ccgtgttcgg catgcagcag
    19561 acggtcgtga agccgcccgc ggcggccgcc gcggagccgg acgcgatcgt ctccttccac
    19621 tcgaagccgg gctcgcggaa gtggacgtgg aggtcgacga agccgggggc gacgacgagg
    19681 cccgaggcgt cgaccgtctc ggcgccgcgg ggaggcgcga tccgctcgtc cacgcgcgcg
    19741 acgacgcctc cctcgaggag gacgtcgagc gtcgcgtcga ggccgcgcga cgggtcgagg
    19801 acccgtccgc cggagatcag gacgggggcg tcactcatcg ccgctcactc cgatcgggag
    19861 gtgtcgagcg cgtgaatcgc gcgagcgagg aggatgtcga gcgcgtagat cgcgcgagcg
    19921 aggaggaagt cgagcgcgtg aatcgcgcga gcacggagtg agcggccgcc ttcgacatcc
    19981 tattcatcgc cgctcactcc gatgagcagg tacagcaccg ccatcctcac cgccacgccc
    20041 gcctcgacct ggtccaggat gacggagccg ggaccgtccg ccacggcgga gtcgatctcg
    20101 acgccgcggt tcatcgggcc ggggtgcatg acgatcgcgt ccttgcgcgc gagcttcctt
    20161 cgctcgggcg tcaggccgaa gagctcgaag tactcgcggt ccgacgggaa gccgaggccg
    20221 ccgccccgct ccttctggac gcggagcatc atgacgacgt cggcgccgtc gacggcctcc
    20281 tcgatccgcg tcgtcacacg cgcgcccgtc cgctcgatct cgggcggaag gagcgtccgg
    20341 ggccccgcga gcgtcacctc ggcccccatc ttcggcagga gcgcgaggtt cgagcgcgcg
    20401 acgcggctga agcggacgtc gccgcagatc gcgacgcgca ggccgtcgag cttccccttc
    20461 gccttgcgga tcgtgaaggc gtcgaggagc gcctgcgtcg ggtgcgcgtg cgtgccgtcg
    20521 cccgcgttga tgaccgcgca cttcaggttc cgggccagga agtggggcgc gccggaggcc
    20581 tggtggcgga tgacgatggc gtcgggcgcc atcgcctcga ggttccggca ggtgtcgagg
    20641 agcgtctccc ccttcgaggc ggacgaggtc gccgccgcga aggagatcga gtcggccgtg
    20701 aggcgccgct cggcgatctc gaaggagaag cgggtccgcg tggagttctc gtagaagagg
    20761 ttcgcgacga ggcgcccgcg gagcgtcggg accttcttga tcgtccgctt gttgatctcc
    20821 gccatcgact cggccgtgtc caggatcatc tggatctcgg ccttcgagag cgattcgatg
    20881 tcgaggagat ccttcttcgt gaaggaggcg gggtcggccg gggtctcggg cttcctgctc
    20941 accgcttccc gcggggccgc gccttcgggg cggccttcgg gacgggcttg cccgcccgcg
    21001 ccggggtctt cttcgccggc gtcttcctcg cccccttcgg cgccgacttg cccttgcgcg
    21061 ccgccgcggg ctccgtgtgg cggtcgagca gccagacgtc gtcctcgccg tcggtctcct
    21121 tgaacgtgac cgagacgact tccgtggcgg aggtggagat cttcttgccg acgagctgcg
    21181 cctcgatcgg gagctcccgc cggccgcggt cgacgaggac cgcgaggagg atccggcgcg
    21241 gacggccgaa gtcgaggatc gcgtcgatcg cggcgcggac cgtccgcccc gtgtagagga
    21301 cgtcgtcgca gaggacgatc gtcttcgtct ccaccgggaa caggatctcc gtccgcttca
    21361 ggaccggcga cgaggccacg gtcgtcaggt cgtcgcggta gagcgtgatg tcgaggaggc
    21421 ccacggggac gcggacaccc tcggccttct cgatctcgag cgcgagcctc cgcgcgagcg
    21481 gcacgccgcg ggtccggatg ccgacgaggg cgaacccgtc gacgccgccc gcggtctcga
    21541 cgacctcccg ggccatgcgg gcgacggcgc gcgccatcgc cgcgggatcc atcagccgga
    21601 gcttgcggta ggtgccgttc gcttcgccca tcgctcgaat cggcgggaca gtacacgggc
    21661 cgagccgccc cgtcaacgaa tgccgggacg ctgaccgatg ggccgtgccg tgtcctccgg
    21721 aggcctcgct gccgcccgac gactggccga ggtagattcc ccgcccatgc gcccgttgcc
    21781 gtctcccgcc cgatcggagg gtgcagtcgc gatgctcacg gacgctctcg cgatctccct
    21841 cccgatcgcg ggtgtcgcga tccgcacgct cgtagcccgc gcgagcccgc ttccgcccga
    21901 ccgccccgcg ctcttcctct tttccgggtt cgtgcaggat ctcgccgcgg ggcttctcct
    21961 cgcgctactc gcgcgcggcc tgaggctcgc cggtgcccgg cccggggcag ccgcagcggt
    22021 aacggccgtt cccttcgcga tcctgctcgt cggccacgcc gcctgggcag aggcgatcac
    22081 cttcttcggg cacccccctc gacgggagga cctggaggtc gcgctcaatg ccgagctgct
    22141 gacccgttcg gccgacgcgc gcgcactcgg tcggctcgcc tcattcctgg ccgtcggagc
    22201 cctcggtacg gtcctcgtca gccacgcgct cgcccggtgg aggcgggcgt ccacgcgaca
    22261 gatcttcgtc ggcgcgacgc ttgcgggggg cctcgccctc ctgatccacc agctggagcc
    22321 ggtaccgacg gcgaacggcg tcctgttcgc cgcgctggac ttcgcccggg agccccgacc
    22381 cgagaccggg cggggaatgg caacggttcc ccctccacat tcggcccccg aggcgatccg
    22441 tcggctcgca ccggggcgag cctgggtcga cccgcatttc ccgctcgcct tcgagccttc
    22501 ggcgcgccgt gcggaggcgc cgcgcgtcgg cgagggagtc cgaccgaacc tcgtcttcat
    22561 ccttctcgag ggggtccggg cgcgcgagat ctcctgctac ggcggaccgg taccgggcct
    22621 gactccgaac ctcgaccggc tcgcacgcga aggaacgcgc gtcgcgcgcg cctacagccc
    22681 gggcacgttc actccgcagg gagaagccgc ctactggtac ggtctcctcg cgacgcccgg
    22741 cagcctcctg ccagtccaga atcccgccac accgctgctc ggccttcccg agatcctcgg
    22801 tgcgaacggc tggcgatcgt tcctctggat ccacaacgga gacgagagct tctatcgccg
    22861 ggaccgcttc tacctttccc gcggatttcg gatggtggat ggccgggact tcccccggag
    22921 cgccgtccgg acgaactggg gctgctccga ccggtcgctc gctcggctcg cggtcgacag
    22981 cctcgaccgg acacgggagc cgttcgcggc gatgctgctg accgtctcga accaccatcc
    23041 gttccaggtg ccgtcggacg ctcgctcggt cgcgccgatt ccgtggactg aggagcgcgg
    23101 ctggttctct ccggctcccg gccttcccct cgtcgggagg cacacggtgc cgatgatgaa
    23161 gacgatccac tacaccgacg aagccctcgg cgacttcttc cgcgaggccg cttcacgaga
    23221 ctgggcccgg cgaaccatct tcgtcatctc gggcgatcac gggcttccga tcgtcccgct
    23281 cggcggcctc ggctccgccc acgagctcgc cgagctgcgt cacggagtgc cgctcgtcat
    23341 ctggtccccc ctgcttcccg gcggccgcgt cctcgagggg ccggcgacgc tcgccgacgt
    23401 ccctccgacg ctcctcggcc tcctcggaat cgacagcccg cgtcccttcg cgggcgtcga
    23461 cctcctggac cccgatctgg acctggccga tcggcctctg cccctttacg acgaagaggc
    23521 ccggcgcgtc accgtcctga ggggccgcct cgcctaccac gcgcggctcg agccgccccg
    23581 aacgtctccc gactggactc tcgggaacga gctcctcgtc gacacggtac gcgatccgcg
    23641 cggcgtcgag aacgccgcgg acctcctccc gcacgagacg gctcgaatgc gccgggacgc
    23701 cgaggtcttc gtcgacgtct acccgtggct cgcgctcgcc ggccgaacgg gccttccgcc
    23761 ggagaggtga caatcccgcg gtgaacgtct cggacttcga cttcgacctt ccgccggagg
    23821 cgatcgccca gcgccccgcc ccgcgcggga cgtcgcgcct catgaccctc gaccgcgcca
    23881 cgggcgcgac gggccaccgg agcgtcgcgg acctccccgc gctcctgcgc ccgggcgacc
    23941 tcctcgttgt gaacgacacg cgcgtcgtcc cggcgcgcct cttcggcctc gacgagacgg
    24001 gacgtcggac ggagttcctc ctcgtcgaga agaccgaggc cccttccgcg tgggactgcc
    24061 tcgcgaagcc ggggcggcgc gcgaaggcgg gacggacgtt cgacttcggc gccggcctcc
    24121 gcgggaccgt cctctcgaag ggggagtccg gccactaccg cgtcgccttc tccgggacgc
    24181 cgctctccga ggcgctcccg ctcgtcggga cgacgccgct ccccccctac atccaccgcg
    24241 acggcggggt ggcggacgcg cgcgacgccg tcgagtacca gacggtctac gcgcgcgagc
    24301 ccggcgcgat cgcggcgccg acggcggggc tccacctgac acccgagctg ctcgatgcgc
    24361 tccgcgcgcg cggcgtcgag gtgtgctccg tgacgctcca cgtcgggatc ggcacgttca
    24421 agccggtgaa ggtggagcgc gtcgaggagc atcggatgga cacggagcgg ggcgtgatcc
    24481 cggaggagac cttcgtcgcg gtgcgccggg cgagggagga gggacggcgc gtcgtcgccg
    24541 tcgggacgac gagcgtccgg acgctcgagg cttcggcgcg cgcgcacggc ggcgacgtcg
    24601 cggccgggcc gttcgagacg cgcctcttcc tcgtccccgg cgcccagttc caggtcgtcg
    24661 acgcgctcct gacgaacttc cacctccccc gttcgacgct cctcatgctc gtcagcgcct
    24721 tcgccggccg cgaacaggtg cttgcggcgt accgggaggc cgtgcgatcg ggctaccggt
    24781 tcttctcgta cggcgacgcg atgctggtcg cctgagcccg ccgggcggcc gtcgggaagg
    24841 gagcgtcggc gacccgcgcc tcagcgcgtc gggaaagcgg cgcgaatcgt ccgctcctcc
    24901 tctccggtca tccggggggc ccccttgcgg agaaggcgga cgaagtgatc gacctcctcg
    24961 tccttcgacg ggaagccgac gttcgttccg ggcccggcca cgttcgacgt cgcgatcggc
    25021 tgccgggtcc gcgcgtccag gacggcgacg aaggggaggc cggcgtcgct ccccgcgagc
    25081 gagtcgagga gcgtctccgc ccccgcgttc tccgtctcct tccgttcgcc gcgctcgaag
    25141 accgtcagct cggtccggac gtaatgccgg ctcacgaccg ccgcgacgtc cggcgtcgcg
    25201 aggaccgcct cgagcctccg gcaccagccg caccacgacg cgtggaagag gacgaggacg
    25261 ttcttcttct cccctcgggc ggccttcgcg gcggccgcga ggaccgcctc ggcgggcggt
    25321 gcggccggga ccacggcggc tgccggcgcg gcgacggcca ccggcgaggc gagacagccc
    25381 gccacgatga aggcagcgac gatgagacgg cggctcgaca tggccgcgga gtgtaaggcg
    25441 cgcgcgggtc cggtgcccgg ccctcgtgtc cgggggccta ctcgccggcg aggatccgga
    25501 ggactcgctc ggcctcggcg gccgtcgaag gcctccagcc ggttccgaac ccggccccga
    25561 ggagcttccg gagatcgcgc acctccatcc cctcgagggc ggcccggagc ttcttcgcct
    25621 cgggcgcgac gttccgcttc tcgtcgtccg ccgcatcgcg gcggaaggcg cgcgcggcgt
    25681 cctcggagtc ggcgtactcg gtcgtccagt cttcgtggag gtagccgcgg agcgcgtcgc
    25741 gcagcgcggg ataggcgcga acgtcgaccg tcgcgggcgc cttcttcgtc gtcgccgtca
    25801 tcgggaggcc tccggataag cagtcaggac gtagtagatc tcggcgtcga cgttccagcg
    25861 gaggacgacg acggcccgcg tgcaggggac gggctctttc gccccgcgcc ggagcgaccg
    25921 accgacgacc ttccgaccgt cgccgggcca gtcgatgacg aggttcgcgc gcgagccctc
    25981 gcggttcgtc caccgctcga tccgcttccg gctcccgcgg atcgcgtcgg cgacgacctc
    26041 ttccgccgtc gcgcggtcgg tgaaggtcga ggccgccgag agccgtcgct cgcgacggag
    26101 ccgctcgcgg agctgggcgt cgctctggcc gacgtgccgg gcgagcgtgt ggccgccgcg
    26161 cttctcgtcg cggaggaggt cccgcttctg cgcgccgtcg gcaggggccg tcgcgccgag
    26221 cagcgcggcg agggcgagga ggacggggac gatcgggcgg aggcgggaca cgagacggcg
    26281 gagcaccacg cgcggaggtt agccgaaagg agcgcgtccg ggctcgccga tcgtcgagcc
    26341 ggtacgggag gggaccgttc ccggatccct gaacgccctc gcggcgggcc tccgcgccgc
    26401 gagccgcccg gtcgcgttca gcggggcgga gcgagatcga gagcgcgagt caggagcggc
    26461 ggcccggcga gctgccccgc gtcgaaccgg aggagctcgc cgtcgaccgt gccggcccag
    26521 agggtgccgt cgggcaggag cgtcgcggtc tgaaggccgg cccccgtccg gcggcgcgac
    26581 caggccctgt aggtgaccgt ccggcgtccc gccgcgtcgg ggagcggggc cgagaacgag
    26641 taccgcgaga ctccgtcgag cccgacgacg aggacgtcgt cctctcccgg caccggcacg
    26701 acccacatcc cgtcgaggcc tgccgcgtcg agcggggaga gcgtgcccga ggcgaggtcg
    26761 aagaggtgga ggctcgacgg ctcgatgccc gccgcgacgt agagcccgag cctcggcacc
    26821 ggcgcgagcc ggtagaccgc ccgcgacggc acggggtgct ccttcacgct ccaccgacct
    26881 tcgcgcgccg tcagctcgag gaggctcgag ctccaggtcc cgacgaggat gcggagggac
    26941 ccgggcagcg gcgccaccgc gtggagcgag accttccgcc cgaggagcga ggcggcgtcg
    27001 acgatcgcga ccggatcgag agagggccag cgccagcgcc gcaccgtgcc gtccgacgac
    27061 gccgtgacga gctcgcgccc gtccgggcca tcgacgaccg tcaggtcgtt caccaggaag
    27121 tcgtgggccc gggtcctgag cttcagcgag ggtccgggaa gcccccagag ggcgaccgtc
    27181 ttgtcgtcgg acgaggtggc gaggaggtcg ccgtcgcgca ccatcgaggg gatcccgtcc
    27241 gtatgcgcgc tcgcccggag aatcgcgagg ctcgcggtgt cgagcgacca gagctcgccg
    27301 tcccggccgc cggcgaagac ggtcatcccc gcggcgtcgt gctcggcgga ccagagcggg
    27361 cgtcctccgg ccggcactcg cgccgtcggc gtgatccccg cgtaggagac gaccgcgagg
    27421 cgctgccggc gcggaaccag ggcgagaatc gcgtccgagt cgacgtggag cccccctccc
    27481 ggcgagacgg gggcgaggat cgcggccggc tcctcgtcgc tgcggtaggc cgtgatccct
    27541 tcgagaccgg aggcgagaag cgtcggggcg tcgccgaccc aggcgaggcc gtaggtccat
    27601 ccgcgccgcg ggagagactt cggagcgagc ccgtcgcctg ccgagaagac ggcgacgaag
    27661 tcgaacggcc cgccgaccgc gacccgtccg agggcatcgt cgaccgccag ggccgacacg
    27721 cgctgctccg cgaagggcgt cacgaagagg gcgttcccct ccggcaggcc gaacgcgcgg
    27781 accggtcctc ctcccccctg ggccagggcg acgagccgtt cgccgacgtc gaggacttct
    27841 ccctccacga acggcagatt cagccgaagc ctgcgagacc agcccgagcc ccccctctcg
    27901 aagacctcga gcgcctcgtc gccgcggacc gcgaggttgc gcccggagcg cgagcagcgg
    27961 acccggccgg gacgggggcc gggagcgtct ggggggagga gcccgaggtc ggcctcctcc
    28021 ggcttcgtcg gatgcctcgc caccacacga cgcccccgca cgagcaggac ccgcccaccg
    28081 ccgaggggct cggcgaggac ttcgtcccgc tcgaggggaa cccggacggc cgggtgcgcc
    28141 ccgtccgccg gaaggaggtc gtaggccgaa cgagcggaga ggacgatcgt cccgtcgagg
    28201 gatccgagga cggactgcgc gtcgggaggg acggcaatct cccgcgtgag cgagagcagg
    28261 ggctcgactc cggcggcggc gccctcagat cccccgcgcg ccgggctccg gagaaggatg
    28321 gacgcgatcg cggccgccgc gagcgtcccg gccacgagga cgccgaggag ggacgccgca
    28381 gggcgcctcg cggacgcggc cggagggtcg ggagaggctc cggaaagcgg ctccgcggcc
    28441 tcgtcggtct cctccggccc ggttgacggc aacgggaccg gggagggcgg tgcctccggt
    28501 cctccggctt cggccgaagt gacgggcagg ttcagccggt atccgaatct cgggaccgtc
    28561 tccagcatcc ccgtcccctc cgcctctccc agggctctcc ggacgaggaa gacggcctgc
    28621 gtcagagagg cgtccgtcac cacggtgctc ggccagacgg cggcgaggat ctcctcccgc
    28681 gggatcgcgc gcccggcgtt ccgggcgagg tgcacgagga cgtcgaaggc cttcggcgtc
    28741 agcggaacag gcagtccgtc cttcaggagg agacgggccc gcgcatccag gcgaaaccct
    28801 ccgaaatccc ataaccatct gttttcgcga gggttatccg acatcacaaa atcctgtgag
    28861 gcgatcctag ccgaaaattg atcggtcccc gaggaactcg tgcgcgtccc gaccgtacgt
    28921 tcgggcgcca tgagacgaac actcagaacg cgaccgacct tcctcggcgc gaccctcgcg
    28981 ggagcggccc tcgccgtcgc tctaccgtcg gccgccgcca acgtccttca gaacggctcc
    29041 ttcgccaccg ggctcagtgg ctggaagatg accgccccgg cgtcctggga agcggagggg
    29101 gcggccgggc ccggctcggg gtcggcgcgc ctggggggga accccggtat cgtcggcgcc
    29161 ctctcgctct ctcagtgcgc cgccgtcacc cccggcaccg cctacgacct cggcgcgacc
    29221 gcgcttctcc cccactcgcc ggaagcgcag ggcggcattt ccatccgcct cgtctggcac
    29281 tcgtggcccg gctgcgaggg ggcgaccctc ggtctcgccc cggccctcga cttcccgcac
    29341 gtggccccgg ccggctggac ccgcaggagt ctcgagaggc tccgggcccc gtacggcgcg
    29401 gcgagcgcct cgctcctggt cgtcccctgg tcctcgggct cggagtccgc gttctccgcg
    29461 ttcctcgacg acgtggagct ctccccttcc tcgatgttcg aaaccctcac gctcccgacg
    29521 gcggcctcgg tggacggagc gaacggcgag cgtttccaga cgacgctcgt cctgacgaac
    29581 gtcgcgctct tcacgcggcg cgtcgacgtc acccttcgct gcccgtcgag ccagccctgc
    29641 gggacctcgg cggtgagcct ccagctcgcg ccgggggaga cgcgcgtctt cgccgacctc
    29701 ctcctcgacg tcttcgggaa gcggaacggc gccggcgcag tcgaggtgac ctacgaggcg
    29761 actcccggtc cgatcgtcgt gtcggcgcgg gcggcgaccg tccacgcgga gcggcccggg
    29821 aacggaatgg ccctgccggt ccttccgacc tcggcggtcc ggagctccgc gacgttcttc
    29881 ggcctgacgg acggccgggc cggggcgggc gggacccgcg tgaacgccgg cgtctacaac
    29941 ccgtcggaca cccccatctt cgtcggcttc tcggtccaca acgagaacgg cgacgtgagg
    30001 aatacggtct cccggtcgat cgccggccgc gaatgggccc agatcgacga cctcttctcc
    30061 cgggccggtc tcgggtcggc cactccgggc tcctacgtga cgttccaggc cgaagccccc
    30121 gtcttcccgt tcctcatctc catcgacaac cgctcgggcg acccgacgtt cctcgagccc
    30181 acggagagct tcctgccatg agacccagcg ctgcattcct ccacgccttc gccgtcctcc
    30241 tcgccgccgc ccttccggcg tccgcgtcgg gcgccgagaa cctcgtcgcg aacggcgcgt
    30301 tccgcgaggg agtctccgac tggtcgcgaa tcgacgtcgt gtcgtacagc gccatggggg
    30361 ccgcgacgcc gggctcggcg cgcatcaccc ttccgagccg ccccgaaggc ggggatgcga
    30421 tgctccagtg catcgccgtc gagggctggc gcctctacga cctcggggcc gccgtccgcc
    30481 tccccgagtg gccggagcac ggcggaggcg tctccgtccg cctccgctgg cacgccctcc
    30541 cgaactgcca ggggccgtcg ctccgcggcg ccccgtcgct cgacttctcc tacaccgagc
    30601 ccgccggctg gcaggtgaag gagagccggc gcatcccggc gccgcaggag gccgtcagcg
    30661 cgctcgtcgt cgtcgtcgcc cgcgccgcgg gagaggagcc ctacccgatc ttcgtcgacg
    30721 acgtcgtcct ccgccgctcc gtcgaggagc aggacgcgtt cctcccgacg gccgcctccg
    30781 tccgtggcgc gaagggcgag cgcttcgaga ccgacctctg ggtctacaac ccggcacccg
    30841 ccgagcggat gttcacgctg cggctctggc gggacgggca gccgacgacg cccgtcgtcc
    30901 tccgcgtcgg cgccaagcag acgcggcacc tctccgacgt cctcctgagc gacctcggcc
    30961 agcgcgacgt cgcgggggcc gtccagctct cgttcgaccc ggggggcgga ccgatgcact
    31021 tctcggcccg cgtcgtgacg gtgaaccccg agagcccggg caacgggacg ctcctccccg
    31081 tccttcgcag ggacgaggcc cgcacggacg ccctcttccc aggcctttcg ggcgctcgtg
    31141 acgatctcac gggcgccttc cgggtcaacg ccggcgcctt caacccccat gacgcccccg
    31201 tcgaggccgt cttctggctc tacgacggcg acgggacgga gctcggcacc ttcacgcgaa
    31261 cgctcgcgcc gggcggctgg ctgcaggtga acgacgtgtt ccgcgaggtc ggcgccgagg
    31321 gcgtctcgac cgagggggcg gtgctcgcgt tcaacgcgcg ccttcccgtc ttcccgttcg
    31381 tcatcgcagt cgacgatcgc tcgggtgacg gcacctccat caaagcgcgc gactacccgg
    31441 tcctccgctg atccacgacg acggccgccg ggagtccgat cctcccggcg acccgtctgc
    31501 gggcggccgg agcggacccc cgtgaacctg tcggggggct tctccgtttc tcccgccatg
    31561 ccgctccccc gctcgcgttg gctccgtatc ctcggcgtct tcctcgtcct cgccgtcggc
    31621 gcggccgggc ttcggaccgc cctcgagcct gaggagaccc cggcgcccgg actgacgctc
    31681 ccggaggcgg agcgtgaccg aatcggggcg ctcgcgcgcg cctcggccgc ggacgccggg
    31741 cgacgcctcg tggacctctc cggcgtggac gtcctcctgg tcggcgagga gcacttctac
    31801 gtggagaccc aggcctggct cgcgcggctc ctcgacggcg tgacgaaccg caggatcgct
    31861 ctcctcctcg agctcccgcg cggcttacag cccgaggtgg accggtgggt cgcgacgggc
    31921 cgctccgcgc gcctcgaaga ggcgatccgc tcgcgggacg ccctcccctt cggcgacctc
    31981 ctcgcctggg ctcaccaaaa ccgctcgcgc ctctcagcgg tcgtggcgat ggacgaggac
    32041 gggaaccgga tcttctggaa ccgggtcttc ctgcgcgaca cgcggaaccg gacgatggcc
    32101 gacgcggtcc tctcggcccg ccgcgcgggg ccgggagacc tcgtcgtcgc ctacggcggg
    32161 cagatgcaca tgctgctcgc cggccgctac cgctacgacc ggaaggaccg cgtcccggcg
    32221 ggagcgcggc tgctgcgcga gggcgtcccg cgggagagga tgcggagcgt cctcctctcg
    32281 ggcgagggga aagcgcccgt ctgcgaggcc tgggccgggc ccggcgcgct cgacatggcc
    32341 gacgccatcg gcgccgagcc ctgggctctc ttcgtcgact atccgctgtt ccgcgtcggc
    32401 gcgagccggg acctcttcga cttcttcgtc cacctcgggc ccctcacgcg cgtctcccgg
    32461 cccgaggccg gctcccgctg agggccgcga cccctcagcg gggcgcgcgg tccgcgacga
    32521 cggccgcgac gaggaggccg agggcggcgg cgtcgagcgc cgagaacgcg aagcccgaga
    32581 ccgcgtagag ggctccgagc gcgcccgggt tcgaggcgag ggtcggcgcg gccgcgtaga
    32641 acgccgcgcc gccgaggctc gtcgcgagcg ccacggcgag ccccgcggcc gcgaggctcg
    32701 aggccttcgg gtgcgccggc ttccgcccca ggacgacgac gaagccgacg acgagcacgg
    32761 cgagctcggg cagctgccgc gcgaccgacg agaggaacgc caggaggggc gacccgcttc
    32821 cgtacatgcc gcggacggta gcagagccgg cgcgtcgtcg ggacgttgac agattcgggg
    32881 ggcgcgcccg atcgtcgggc catggccgct ccgtctccct cggaccgcct cctcgagatc
    32941 ctcgccgcga agatcgccga agagacgggg ctcgatccgg ccctcgcacg cgaggtcgcg
    33001 ccggcggccc tcgcctcgga ctccgtcgac gaaggcgagc cgatcgcgct ccgcgacgcg
    33061 cgcggccacg tcgtcgcgcg catccccttc tcgctcgtcg aggaagcctt cgacgacctc
    33121 gacgacgagg aggacggcga gcgctgaacg ggcgcccgcg gttctcctca ccgcgggcgg
    33181 ggcgcacggg aaacccggcg gcccgggttt cccttcgtcc cgcgctcgcg cagcgagcag
    33241 ccccaccgcg cggaaggggg cccgggggaa acccggcggc ccgggttccc cccgatcacg
    33301 tcagtacgcc tgcaggtact cctccacctc ccaggggtgg acgcgggaga tgtaggtcgc
    33361 ccactcggcc cgcttggcct cgacgaagtg ctggtggatg tgctcgccga gggcgttctt
    33421 gacgacgtcg tccttctcga ggcagtcgag ggcgtcggaa aggttcgccg gcagctgctc
    33481 gatcttccga cgccgcttct ccctctcgga catttcgaag atgttctcgt tcaccggctc
    33541 gcccgcgtcg atccggttct tgatcccgtc gagaccggcc gcgagcatca ccgcgaacgc
    33601 gaggtagggg ttgcacgcgg gatctggcat ccggacctcg cagcgggtcc ccatgccgcg
    33661 ccgcgcgggg atgcggacca tcggcgagcg gttcttctct gaccaggcga cgttcacggg
    33721 tgcctcgtag ccgggcacga gcctcttgta ggagttcacg agcgggttcg tcacggccgc
    33781 aatcgccttc gcgtgcttca ggacgccgcc gatgtagtac atggcggtct tcgagagctg
    33841 gtacggcgct ttcgcgtcga agaaggcgtt cttcattccg gcgcccgtgc ccgtcatcag
    33901 cgactggtgg acgtgcattc ccgagccgtt gaccccgaag atcggcttcg gcatgaacgt
    33961 cgcgtggagg ccgtggtcca tcgcgacctt cttgacgacg agcttgaagg tcgtcacctt
    34021 gtccgccgtc accaccgcgt cgccgtactt gaagtcgatc tcgtgctgcc cgggagccac
    34081 ctcgtggtgg gccgcctcca cctcgaagcc catcgcctcg agcgcgacga cgatgtcccg
    34141 ccgcgcgtcc tcgccgcggt cgacgggggt gaggtcgaag tagccgcccg agtcgtgcgt
    34201 gtcgacgacc gggtctcccg aggcgtcccg ccggaagagg aagaactcgg cctcgggccc
    34261 cgtcatgagg gagaagccga gctccttcgc cttctccctc tgcctcttca gcgtctggcg
    34321 ggggcagccg gcgaacggct tcccgtccgc ggtgatgacg tcgcagacga gccgggcgac
    34381 cttcccgttc gtgtgcgacc agggaaagat ccggaacgtc tcgaggtcgg ggacgagccc
    34441 catgtccgac tcctcgatcc gggtgaagcc ctcgatcgag gagccgtcga actggatctc
    34501 cccgtcgagc gccttctcga actgggacct cggcacctcc acgttcttga tgatcccgag
    34561 gatgtcggtg aactggaggc gcaggaaccg caccccctcc ttgtcgcacg tcttcaggat
    34621 ctcgatcgcc ttcgtcgcca tcgcgcttcc ccttcccttc gagcccggtt ccaggatggc
    34681 gcggaatttc tgccaccggg cggatctcgt caagggaaat ctcgaaaatg gcgctcacct
    34741 tcggcacaat acgtcgaatc ggaccagaca atctgctttt gtggcgaggc cccggacgcg
    34801 ggatactccg cgccatgacc gcaccgaccc gcgagaacgc ttgggccctc ctgaccgagt
    34861 tcaccgcctc tcaggcgctc cgccgccatg ccctcgccgt cgaggccgcg atgcgcgctc
    34921 tcgcccgctc cgccggcgtg acggacgaag ccgagatcga gcgctggggg ctcgtcggcc
    34981 tcctgcacga cttcgactac gagaagtacc cgaccgaggc ggaccacgtc ttccgcggga
    35041 tggagatcct ccgtcagcga gggtggcccg aggacctgat ccgggcggtc ggcggccacg
    35101 ccttctacac ggggatcccg cgcgagacgc cgatggagaa ggcgatcctc gccgccgacg
    35161 agctgaccgg cttcgtcggc gcctgcgcgc tcgtccgtcc ctcgaagcgg atcgccgacg
    35221 tccccgtcga gtcggtgatg aagcgcctca aggagaaggc gttcgcggcg aaggtcgacc
    35281 gcgagtacgt gaggcggggc gccgaggagt ggggactgcc gctgcccgag ctcgtcgcga
    35341 tcgtcctcgc ctcccaggtg cccctcgccg acgcgctcgg cctcggcggc gcgcccgcgc
    35401 ccgacctccc cgacgccccc tgcccccctc cgccgccggc gccctgagga gagccggacc
    35461 ggtccgcccc tccgctcgac gcgagccgcg cgcgggccga tactccgccg gatgcgcgct
    35521 cttctgatcg agaacgccct cgtcgccggg atgcgctcgg ccgacgacct ccacccgcgc
    35581 ggcgcggtgt gggcggagga cggcgtcatc cgcgccctcg gctccgaggc ccgggccgct
    35641 gccgcggccg ccgccgcccg cggcgaggcc gtcgagaggg tcgacgccga ggggctctgg
    35701 ctcttccccg gcttcgtcca gacccacgtc cacctctgcc agacgctcct ccggaacggc
    35761 cccgacgacc tccccctcct cccctggctg aagacccacg tctggcccgg cgaggcggct
    35821 cacgacgagg agaccctctt cctctccgcg cgcctcggcc tcgccgagct cgccgccggg
    35881 ggaacgacgg cgatcctcga catggggacc gtccgccata ccgacgccgt cttccgcgcc
    35941 gcgcaggcct cgggcctccg cgtgacgagc ggaaacgccc tgatggacga cgcggcgatg
    36001 aacccgccgg aactcttcgc cgagacgtcc gaggcgctcg aggagggaga acgtctcctc
    36061 gcccgctggc acggcgcggc gaacggccga ctccgcttcg cctggtgccc gcgtttcgcg
    36121 gtctcgtgca cggaccggat cctcgaggag gcggccacgc gcgcgaaggc gcagggggtc
    36181 ctcgtccaca cgcacgcctc cgagaacgtc gacgaggtcg cgctcgtgag ggaacggagc
    36241 ggccgcgcga acatcgagca cctccgcgag gtcgggatcg gagggcgcgg gaccgtcctc
    36301 gcccacgtca tccacacgag cgaggaggag cgccggctcc tcgccgccga cggcacgggc
    36361 gtcgcgcact gcccctcctc gaacctgaag ctcgcttccg ggatctgccc ggttcccgac
    36421 tacctcggcc gcggcatccg cgtgacgatc ggcgccgacg gcgcaccctg caacgaccgc
    36481 ctcgacgtct tctccgagat gcgccacgcg gcgctcctgc agaagatcgc ggcggggccg
    36541 ggcgcgttga ccgcctggca ggtcgtccgg atggcgacgg ccgagggggc cggggcgctc
    36601 gggctcggcg acgtctgcgg gacgatcgag gtcggtaagc gggccgacct cgtcctcctc
    36661 gacccttcgg ccggcttcgc cctcccgacc tcctggcgcg acgacccgta cggacccgtc
    36721 gtcttctcct tcgaccggag caacgtcgtc gagacgcgcg tcgacggcga ggtcgtcttc
    36781 caccgcgagc ggcgcggagc ccgggtgctc gcgccgacgg ccgccgagat cgaatccgca
    36841 gtcgcgcgac ttcgcgagtc ccgcgtccgg aacgggcgcc gcggggccgg agcctcttga
    36901 tgagagcgcc gctccggccc agactcgggc cgggagatcg ctggaggtga acgatggccg
    36961 cacgtgagaa cgccgctcga ctgaagagca tcgtcgtcgg caccgatttc tcgatctgcg
    37021 ccgcccgcgc cctcgctcac gccgtttcgc tcgcgcagct ctcgggcgcg aagctgcacc
    37081 tcgtccacgt cctgctcgag ccggtccagg cgctcgacgt cgccgccgcg ctcccctacc
    37141 ccgacgtcga ggtccggagg gagtgggagg aggccgcgcg cgtcaagctc gaccgcgagg
    37201 tgaaggcggc cgagaagcgc ggggtcccga cgacggcgga gatccgctgg ggccgcccgt
    37261 ccgacacgat cgtcgacacc gccgcgcgcc agaaggcgtc gatgatcgtc ctcgggaccc
    37321 acggccggtc ggccctcgag aagctcctcc tcggctcgac cgccgagagg gtcctccggc
    37381 tttcgcccgt cccggtcctg accgtccgag agaagcggtg accgcgcgcc ggggccccgc
    37441 cccggcgcgc ccgtcccggt ggcccggccg gcctagtcgg ccttctccca ggcgtggggg
    37501 atctcgacgg gaaacggcgt cctcgtgagc cgggtccggt ccccgaagta gagccccccg
    37561 gccgtcgcgt cgccgaagcc gacccactcc tcgaaggaga cggcgttcag gaggtcctgg
    37621 acggccaccg tcttcgtctt cttcttcggg ttctcgcgga tctcctggcg ggcattgtcg
    37681 taccggctcg aggcggccag ctggggctgg tcggtccgga gctccgcgaa gcagacctgg
    37741 cggaagacct tgcccggccg gtgcgtggcg taccaggcgt actcgaccgg cgtcaggttc
    37801 gagaggacga ggagctcgag cggcgcgaaa atggcgtaga gccgcggctc gcggcgggcc
    37861 ggctcggctg cgccgatcgc ctgcgccacc tcggccggag ccatgctgtc acggcagggg
    37921 gccgggccga accgcgcgac gtcggagacc ggccccatcg agagcgcctg accgccggtc
    37981 ttcacgagca gcacccggcg gtacgcctcg atcggggtga tcgtgaaggc gttcgaggag
    38041 agcgccgtct tcgtcctctt acccgagcgg acctcctgga tcgcgcgggc ggtgtctccc
    38101 gccgtgtcgc gccagccccc ctcctcgagg aaggagaacg ccgcgccggc ttctccgagc
    38161 gccaggtcga cgtagatccc ccgcccgtgg aagtgccggc ccgaaccggg agagaggtgg
    38221 tgggcgagcc cctccacgcc gaggctcgtc gcgatgacgg agccgtgggg gatcgtcagg
    38281 acgaggttca accgttccat cgtgtctcct gcctcccgga cggaccgggg cgcgaaaggt
    38341 acatcagccg ttcggaactt cccagagatc ccggacggcc gggtggtccc aggggatccg
    38401 gtgctcgtcc gggtcgttca cgtcgaacgt ccgggtgacg aggtagaaga gggcgcacgg
    38461 gttcgtcagc ggacggtagc cgtgggcgac cccctccggg atcctcacga ggaggtcctg
    38521 gccgtcgccc gcgacgacca cctgggtccg gaggtaggtc ggcgaggcct tccggacgtc
    38581 gaagaggacg aacttggcct tcgaggggtg cggacagaac cagacgtcct gctgcttcag
    38641 gtggtagtgg agccccttca cgtggttctc cgcgttcacg atcgtgtagt tgagctgcgc
    38701 gaccgggacg tcccgaaaga aggccgcgag ggactcgctc ccggcgtggt ccgccttcgc
    38761 ccggtagatc tcctggaaga acccccggtc gtccacgaac cgggggaggc gctggatcgc
    38821 gactccgtcg atgggaccgg gcctctcgta gctcttcgtc ccggaacgaa actcctctcc
    38881 gatcgcctcg atcgggagga cgggcgtgtc gcggatgctc attccgcgat tctaatcggc
    38941 gcgggaggcg tgctagcctc ccacagcccg atgccgaaga ggatcattcc cgtctcctcc
    39001 gggaagggcg gcgtcggaaa gacgaccgtc gcgaccaact tcgcgctctc gctttcccgg
    39061 ttcgccccga cgatcctcgt cgacctcgac acgggcacct cgtcggtgag gagctcgatc
    39121 ggcgtccccg tcgcgcacga cctctaccac ttcttccgga aagggcgccg cctcgacgag
    39181 tgcgtgaccc gcctccccga ctcgtgggac cccgacggcc tctaccgcgg cttcgggttc
    39241 gtggcggggc cgctgcacct gatcgaggag gtgacgaact tcgggccgga gaagaaggcc
    39301 cggctctgcg acgcaatcaa cgagctcccg gccgactacg tcatcctgga catgaaggcg
    39361 gggctcgacg cgaacgtcgt cgacttcctc ccctggtcga actccgggat cctcgtcttc
    39421 acgccgcacc tcccctcggc gacgctcgcc gcctccgaca tcgtcaaggc gatcctcttc
    39481 cggaagctcc ggatcgtctt ctccccgtcg agcccgttct acgcccaggt gaaggacccg
    39541 cggacgacga cgctcctcgt gaacgacctc ctcgacgagg tcgaggacgt ctacgagccg
    39601 ggcctcccga acctcgacgc gttcctcgtc gacctcgccg cggcgctcgg cgaccacccg
    39661 gtcctcgatc aggtcgcgcg gaccgtcgag cacttccgcg tccactacgt cctgaactcc
    39721 ttcaacggcg tcgacgaggc gttcgacacg gccgtcaagc cgttcgtcga gaacctcgtc
    39781 agcaccgtct ccgagcggat gacgctcacg aacctcggct gggtcgtccg ctcggacgag
    39841 atccaccagg ggaactgccg gaagcgcccc gtcctgctcg cccctcccga cggccgcccc
    39901 gcctcgcgcc ccacgcgcgg cgagcgcgag ctcgacgccc tgatccgcga gacggcgggg
    39961 ctcgcggccg acccgaggcc gcgcaagccg gtcctgcccg tcccgacgcg tcccgatccc
    40021 gagagcgccc tcgcgcggga gctcgacgcg ctcaaccgga tgtaccaccg ccgcgggaat
    40081 cgggacgacc cccgggacaa cttcgactac ctcacggcgc gcgccctgca cctgctcgag
    40141 agggcccgtc cgtccgagtt cgggacggcg cgcctcgtcg gccccgtcga gatcctccgc
    40201 tccttcttcc ccggcggcgc ggcgcgcccg gccgggtgaa ggtctcgggc cgggccgcgg
    40261 ccgtcggcct cgcgatcctc gcgggcgcgg ccgtcctcgc ctcgcgcgga cgcctccgcg
    40321 agctcgggct ccgggcccgc ctcgccttcc tcgcgatcgt caccctcctc ggcgcctcgc
    40381 tcctcgtgcg tgggctggcc gcggagggcg accgccggct ctacgcgctc gtggcgttcg
    40441 cggtcgtcgc gccgtgcgtc gtcctcgcct ggagggacct cctccgccgc cggtagacgc
    40501 ctaccggagg aggacccggt cgagggcgat cccgagacgc ctcgggtcac cgccgaggcc
    40561 cgcgtcgcgc gggacgagga ccggcgtcgc gagcgtgatc cttaccgggt cgccggcgcg
    40621 aaggcgctcc cgtgccggcg gatcgagggg gatacggacc tcggcgaaat cgcccggcgc
    40681 gacctcgacg ctcccggccg cgcgtccgtc gaacgcgacg tccacccgga cgccggacgc
    40741 gccgggcgcc cgggctcgta ggacgagctc gcccggcgcg aggcccggga cgggtaccca
    40801 cgtgaacgcg gcgcggccgg aggtccagcg ccacgtcgcg ccgctcttct cgtggacctc
    40861 tctgtcgtag aaaccgtcga ggtcggtcca ggcgtcgcgc gggcggccga ggtcgaggag
    40921 gcgctcctcg ccgagccgtt cccagacctc ctcgggcggc agggagaacg tcgcctcccg
    40981 gaacaggaag cagccgcgcg agggccggac gagaccggga gcggccggga ccgcctcggg
    41041 gcggtcgcac gacaggacga gctcgcgcgg cttcgggaga ggtccgtaga tcggctgcgg
    41101 caggaaggcg acttcgaagt cgcccggctc gatgcgccgc tcgaggacgg tccttcccgt
    41161 ctcggcgtcc ctcaccttca gcgtcgtcgc cccttcgtga tgctggcgaa ccccgcgcag
    41221 gagcgcgacc ggtggcccct ggagccccgg gacgatgagg cgcgcctccg gtccggccca
    41281 tcgcgtcgag ggccaccccg gaacgctctc cctcggcccg aagccgggtc cgaagagcgg
    41341 cggcgcgggg tcgcgcacgg cgaccacgaa cagccggccg ttcccgagcg cccgggctac
    41401 tcgcccctcg tgccggaaga cgcgccaggc cgggtccgtc cggtcgggcg agtccccctc
    41461 cgacgtgaag tcggcgaggc ggacgagcct tcggttcctc cgcacgccct ccctcagcag
    41521 gatgtcgtcc gagagcggcc agacgcgcag gcgcttctcc caccgttcca gccggaggaa
    41581 cgcgttgaag atcccgtcgg agacgatcgt ctcccggccc ggatggccgt agagcgagag
    41641 ggcgtcgatc gccgccgaag ggggcgtggg gttcgtggcg ccgtagcgca cctcgcccca
    41701 cgaagcccgg ccggcgagag ccgccccggc caccatcgcc gccaggccga gaggaggcct
    41761 ccggacgagg acttcgaccc ccgccgcgac cgggagcgcc aggacgagga cgaacgggac
    41821 cgagtagcgc gacatctccc ggctgtgaag gaaccagagc gaatagaacg ccggggcgag
    41881 gacgaggagg agcgcggccg cgccccgagg cagacgccgg aacgtcgccc cgaggccgag
    41941 gagggcgagc agggcgatga cgagcgcgag cggccacgag aggaagtcgc ggaccaggaa
    42001 cgagtccagg atgctgccga accggccggt ctcgaacgcc cccgagcgga aggaggcccg
    42061 ctcgtcgatg cccgcgagga agccgcgcac accgccggtc tgcttcagaa gccacgcgag
    42121 ccaggcggcc gtacccgcca gcccggacgc gacgagagcc gccgcgacgg ggcgtccgga
    42181 ccgcgaccgg acgaggagga gcgcttccac gaggaggagc ggcccgaacg ccaggaccag
    42241 gtgcgggcga accccggctc cggcggccgc cgcgaggccc gcgaggacgg cgagggacac
    42301 ggcgcctgga cggccggaga ccgcgcgtgc ggaggccgcc ccgagcgccg cgagcgacgc
    42361 gaggaagagc gccgtcgacg gcgtatcgga gaaggccctc ccgccgttca cccagacgac
    42421 ggggaggaac gccgcgaaga cggccgcgcc cagcgcggcc caccccccga cgagccgccg
    42481 tccccagagg tagagcgccg gcaggccgag gcacgagagg agcgtcgcgg ccacggcgag
    42541 agcgtggaat gcccctggag cgagcggcag gagcgcccgg ccgaggagga tccagacagg
    42601 gaagccgggc gcctgcggcg agaggtccgc gatgtcgaac cggtgcacgg ccccggcgaa
    42661 gacggcctcg tcgatctccc ccggcgcggc cgcgatcgcg aggaaacgtg acgcgatcac
    42721 ggcgccgagg agggcgacgg ccaggacggg cggcggctcg ccggcggagg gacgcggggt
    42781 cggatcggcc acgatctccc gcattctagg ggcccggcca actcccgcct tccgggcacc
    42841 tccggccaca ggcgctagaa tggggcgatg ctctcgagga cccgcctgta gggatggccg
    42901 agccacggat catcccccgc gccgagcacc cgatctcgcg cggctacatc gaccccgacg
    42961 ccctgaaggt tctctaccgc ctccacaggg cgggatacaa ggcctacctg gtcgggggct
    43021 ccgtccgcga cctcctgacc gggcgcaccc cgaaggactt cgacgtcggt accgacgccc
    43081 ggccgcagga cgtgcgccgc ctcttccgca acagccgcgt catcggccgg cgcttccgcc
    43141 tcgcgcacgt cctcttcgac ggagggaagc tcgtcgaggt ctcgaccttc cgccggcgcc
    43201 cggacccggc cgacgccgag ccgggacccg ggggcgacga cgacctcctg atccgttcgg
    43261 acaacacctt cggcacgccc gccgaggacg ccgtccgccg cgacttcacg atcaacggcc
    43321 tcttctacga catcgccacg ttcgccgtga tcgactgggt cggcggcgtc gaggacctcg
    43381 agaggcacgt catccggacg atcggcgacc cggagatccg tttccgggag gaccccgtcc
    43441 ggatgatgcg cgcctgcgag ttcgcggcgc gcctcgactt ccggatgacg cccggcgtcc
    43501 gggccgcgat cgaagagaac cggaaggaga tccgcaagag cgcctcgccg cgcgtcacgg
    43561 aggagctcct cgacccgctc cggcgaggct ggggcgcgcc gacgtatcgc ctctggtcgg
    43621 agaccgggct tctcggcgag atcttcccgg ctctcgaggc cgagatcggc ggcgcctccc
    43681 gcgggacggg gctcttctgg aagctcctcg cggagaccga tcggcgccgc ggcggcggcg
    43741 aggtcctctc ggactcgatc ctgctcggcg tcgtcttcct ccccctcctc ttcgccgcca
    43801 tccgggcccg ttccggcgga tccgtcgacg cgcagaagct ccttctcctg ctcgaggagg
    43861 tcgtcaaccc cgaggcggcg cgcctctcgc tgccgaacgc ggcgatccac gacctcaagc
    43921 agggactctt cacgatgggc cggatcgccg agctgcggcc gggagacccg gccgctcgcc
    43981 gcctcgtcac gaaggggtac ttccccgtcg ccgtcggcct cctgtcgctc tactcggccg
    44041 cttccggccg ctaccgcgac gccgcccgga cctgggagga cctcctgcgc cggggaggcc
    44101 ggacgccccg tccgggcgag gacctccgcg cggcgatccc cttccccgag gctctgatcc
    44161 cgcacgagga gacgggcccg acggagcttc tcctcccgga cgaggcgccg ccgcgcggtc
    44221 ggaccgcgcc ccgtcgacgc cgacggcgcg gccgcggcgg tcgggccggc gaagccgcct
    44281 gaggccctac ttcggcgatg cggcgggcgg accgtccgaa cgccgttcga agacgtaggg
    44341 cccgttccgc gctccccaga cctgacgtcc gttgacgtcg tatccgcggt cccacgactc
    44401 cattcgaccc gggcgaagcg tcacctccga ggtcgcccag cgcgctccgg cgagcgcgga
    44461 ggggcacccc ttcccttcgg tcgaaccccg ccagccgtcg tccgtccgca tcagtcggac
    44521 cgcgcagccg agccgctcca cgacgtcgcc cgccccgtaa agggcgaggt ccgacgggtc
    44581 acgccacttc cccgacaccg cgatcggctc cttcggctcg aagacgcgcg agacgacgcc
    44641 gccgtccgcg gtctcctcga tccggtagaa gcgctgccgg tacggccggt ccgggctctc
    44701 gaggagcgcc tgctcgacgt agaggaccgg cgcgcccgcg ccgagccggc tcgccgggac
    44761 ccgcaccgcc acgagccgga cggcccggta cccctccggg tccgagccgg actgggcgga
    44821 ggagtcgaac gtccccgtca ggagagcggc gacctcctcg acccgggggg ggggctccgt
    44881 tcccgccgcc ggaacgcccg cgagaaggag cgtggaaagg gcgaatcgtg cgcgtcgcat
    44941 cgcgccgact ctacggcatc aggaggagga ggctcagcga catcgccccc atcccgagga
    45001 ggaggccgta gagcgagtcg tgcccctcgc cgaaggcgcg cgacgtcggc agcagctcgt
    45061 cgagagagat gaacaccatg acgccggcaa ccgcaccaaa gacgacgccc atgacgccgg
    45121 acgtcaggaa cggacgcagg acgagataac cgacggcggc gccgaccggc tccgcgagcc
    45181 ccgagaggaa cgaccacccg aacgccgcgc gccttcgccc cgtcgcgtag tagatcggca
    45241 cggccacgct caccccctcc gggatgttgt ggaacgccac ggcgatcgcg atcgcgaggc
    45301 cgaccttcgg gtcgtcgagc gcggagagaa acgtcgccat cccctccggg aagttgtgaa
    45361 tcgcgatggc gacggcgacc gccgtccccg tcctccgaag cttcgcggcc gcggggaggg
    45421 ccatcgcgtc gccgagcgac gcgagctcga gcgccgtccg gacctcgtgc gggttctcgt
    45481 cgcccggcac gagccggtcg atcgcgagga tcaggaggat gccgaggaag aacgctccga
    45541 cggagagcca ggctccctgc cgtgcgccga cggccgtcgc gagcgccgcc tgcgacttcg
    45601 gcaggatctc gaggaacgac acccagagca tgacacccgc cgaaaacccc gttgcgaccg
    45661 acagcagccg gaggttcgtc cgccgggcga agaacgcgat cgcgctcccg agcccggtcg
    45721 acagccctgc cgcgagcgtc agaccgaacg cgacccccac gttgcccgcc gtcgtaaccc
    45781 ctccccggcg ccacaggcgc accgatcatg ttcgcacggt cggaaacgac ccgggcgcgg
    45841 cgaacgccgg gcgggacgtc gggccctccg ggccctccgg ctacgcggct tcggtcgtcg
    45901 gcttgcgcgc cttgcgctgg gagtggtcga ggacgcgctt gcggaggcgg atggagcgcg
    45961 gcgtgacctc gacgagctcg tcctcctcga tccactcgat cgcggcctcg aggggcatct
    46021 cgcgcggcgg ggtgagcttg atcgccatgt cggagttgga ggcgcgcatg ttcgtcaggt
    46081 gcttcttcac gcacgggttg acgatcatgt cgttctcgcg cgcgttctcg ccgacgatca
    46141 tgccggggta gcaggcgacg cccgggccga agaagaagac ggagcgctcc tcgagccgct
    46201 cgatggcgta ggccgtcgtg tcgccgccct ccttgcagat gagggcgccc gtgcggcgcg
    46261 tctcgacgtc gcccttccag ggctcgtagc cggcgaagac gtggtggagg atcccttccc
    46321 ccttcgtgtc ggtgaggaac tcgttgcggt agccgaagag gccgcgcgtg ggcaccttga
    46381 actcgattcg cgcgcgcccc gtgccgttgt tcacgagcgc cgtcatctcg ccgcggcgcc
    46441 ggccgatctt ctcgatcacg gtcccgacga actcctccgg cacgtcgacg acgagctgct
    46501 ccgcgggctc gcacgtcacc ccgtcgatct cccgcgtgat gacctccggg cgggagagcc
    46561 cgagctcgaa cccttcacgc cgcatcgtct cgatgaggat cgcgaggtgg agctcgcccc
    46621 ggcccgagac cttgaaggcg tcgggaacgt ccgtcggctc gacgcggagc gccacgttcg
    46681 tccggagctc cttctggagg cggtcccaga tgttccggct cgtgacgtac ttcccctcga
    46741 ggccggcgaa cggcgagtcg ttcacgcgga acgtcatcga gaccgtcggc tcgtcgaccg
    46801 cgatcgggtc gagcgcgacg gggaagtcgg cgtcggcgac ggtctccccg atcgtcacct
    46861 cctcgaggcc cgagaggacg acgatctccc cggcggcagc ctcggcgacc tcggtccgct
    46921 tcagcccctc gaagaccatg agcttcacga cgcgcccctt cgcgatcgtc ccgtcgtggc
    46981 ggcagatcga gacgttctgc ccgggcttca cgacgccgtt cacgacgcgc ccgatcgcga
    47041 gccgcccgag gtagttgtcg tagtcgaggg aggcgacgag gatctggagg ggcgcctcgg
    47101 cgtttccgcc cggctcaggc acctcgacga ggatcgcgtc gaggaggggc ctcacgtcgt
    47161 tgtcggagtg gtcgacctcg cgccgcgcga agccctgctt ggccgaggtg tagacgatcg
    47221 ggaagtcgag ctggtgatcg gtcgcgccga ggtcgaccat gaggtcgaag acctcgtcga
    47281 tcacctggtg gggccgcgcc tcggggcggt cgaccttgtt caccgtgacg atcggacgga
    47341 ggcccagctc gagcgccttg cggaggacga accgggtctg gggcatcggg ccgtcggcgg
    47401 cgtcgacgac gaggaggacc gcgtcgacca gcttgaggac ccgctccacc tcgcctccga
    47461 agtcgctgtg ccccggggtg tcgacgatgt tgatcttcgt cccgtggtag cggatggagg
    47521 tgttcttggc gaggatcgtg atcccgcgct cgcgctcgag gtcgttcgag tccatcaccc
    47581 gctcttcgat gtgctccccg gcccggaacg tcccggactg ccggaggagg cagtcgacga
    47641 gggtcgtctt gccgtggtcg acgtgggcga tgatggcgac gttgcggagc tcgggcattc
    47701 gtcttccttc cccgcgcacc ggcgcgggag cgggagggta ccagacccgc ccggaccggc
    47761 ccgactactt ccgcgagagc accgcgctca cgtagggcgc gaactccttg cccgcagggg
    47821 cgacctcgag cagctccccg atcgtcccgt cggctctgcg ggtgaacgtc cagcgagacc
    47881 ggatgccggc ctccaggttt gccatctcct tcgccgtgaa cacgataccg tcgggtcgca
    47941 cgtccgcctc gtagatcccc cagaccttcg tcgagaagac gacgagggcg acgtacttgc
    48001 cctttccccc gtcgtagacg atgtaggcga cctcttcggt cgagctctcc gggagcgcgt
    48061 ccttggccgg gtagaaggtc gtcgcgcgat agctgagctg ggcgcctccg accgaggacg
    48121 tgaaggagac gacccgaccg ccgacgcgcc cgcccgactc ggccgggccg gcccaggagc
    48181 cgttcaggaa accgaactcc tggaagatct tccactcctg ggccgggggc ggatcctggg
    48241 cctcgaggct cgccccggcg agcagcaggg gcaggagggc aaggagtacg ggaaccctca
    48301 cgtttcgagc agagaacatc tcacccctcc tcgacgaatc cgacgggaac gggaattata
    48361 gactcgcacg ggaacctcac gcccgcgaac tccgtttcga ctttcgagga ggccgatggc
    48421 gaacgccccg caccccgacg ccgagctcgt cgctcgctgc ctgaacggcg acgaaggggc
    48481 gtggcgagag ctcgtggagc ggtactccgc gaaggtctac cggatcgcct accaccggac
    48541 ctacgaccgg gccgaggccg aggagctgac ccaggactgc ttcctgaagg tctgggagaa
    48601 cctcgaccgg tacgagccga ccgaggcctc actgctcgcc tggatcgcga ccctctcgcg
    48661 gaacctatcc atcgaccact accgaaagcg acgccgggag aagggcttcc gcttcgtcac
    48721 cgacgacgcc ctcgtgaccc tcctccctgg ctcggaggac ccctcggccg acgccgttcg
    48781 gcgggagcgg ctccgcctcc ttctcgacgc gatcgcgcag ctccccgacg agctcgccga
    48841 gatcgtccag atgcgagatc tggacggcct cgactaccgg gagatcgccg atctcctcgg
    48901 gctccccgac ggcacggtca agtcgcgcct caaccgggcc cgcgtcgagc tggcccggat
    48961 cgtgagggag aggatcggcg cgacgcccct cgcccgcggc ccgctcttcg gcgggctgac
    49021 gccgcaggcc ggaggcgcca catgagcggc ggcgcggccg tcccctgccg tcgcgccgag
    49081 acggcgctcc tttcgggcgg cgtccccgtc ccccccgagg tcggggccca cgcctcgtcc
    49141 tgcgagacgt gtggacctct cctctcgggc tgcgaggaga tccacgccct gctcgccgcc
    49201 ctcgtcgtgc ccgagccgcc tcccgcgctc tccgggacgc tcgcggcgat cggctcgacc
    49261 gagcctcccc gcgtcgccgc gagagagacc ctggccctcc tctcccccga ggcgctcgcc
    49321 gtccccgagc cttctcccgc cctccttcgc cggctcctcg ccgtaccggc gacaggaccc
    49381 cggcgcgcgg cgaacgtcgt cgagatgagc cggttccgcc gcctgcgcag cgactggcgc
    49441 gtcctcgtcg ccgtcgccta cgccgcctgt ctcgccctcg tcgcgatcct cggcgtcgac
    49501 ccgctctccg ccgcacggag cggcgcgagc ggaatggccg ccgttggaga gcgcgcgatc
    49561 gccgaggcac gcgaggcggc cgccggcaag ctcgacacgg tcctcgcggc ccagaggaag
    49621 aagcccctca ccgagcagat cgactacaag gtctacagga cgctcgccgt cgggaaggcg
    49681 aagacctccg cgtgggccgg catcctcctc ggccgcatct tcggaacgcg cgtcgaggcc
    49741 gaggcgccgg ccgcacctcg ccggagcgaa ccggaccccg gcagtttgcg ttcctttgat
    49801 cgggccggga cggagacccc gctctcgcga gcccagcgag cctgaccgaa gcccagaagg
    49861 tggagccgat gaacgaaaac ccgaaccttc cgccgccccc cgtgagcgcc gccgcggccc
    49921 ctccggtcgc ctacgctccg cccgcaccgc cgcgcctcgc ggcccctccg gggaagaacc
    49981 ccgcggccgc cggtctcctc tcgctcttcc ccggcctcgg gcacgtctac ctggggctct
    50041 acaagcgagg ggtcgtcttc ttcgcgatct gggcgttctt catcgccgcc gtcgaccgcg
    50101 gccacgacat gccgttcggc ctcctgatcc cgttctggat ggtcttcgtc ctcatcgacg
    50161 ccgtccggca ggcgcgcgcc ctgaacgcca ccggcatgcc cgagcccggc ttcttcggcg
    50221 aggagcggcc gttccgggcc gacggcgggc tcacgttcgg catcttcctc atcctgatcg
    50281 gcctcttctt cctcgccgac cggatcttca cgatcgacct ctcgttcctc ctcgactggt
    50341 ggccggtcct cctcgtcgcc ttcggcggct ggcaggtctt cgcctacttc caggagaaga
    50401 agcggcgcga cgaggcggcg gccagccggg aggcgggagc cctcgagccg tagggccggc
    50461 tccacggctc gcgaggggct ctcgcgcgaa agggaggggc cgcggcccct ccctttccgt
    50521 ttcgcgcccc gccgccggga ggctcgtggc gagcctcccc gactcatctc acccgccgag
    50581 actcgtcacg aggacgaccg tcgtctcgga gaccttgccg tcccgcgggt cgatcaggtg
    50641 gtcctccacg acgacctgga gcgtgtcgtc gtccttcgtc cagcgcgccg tccggcccga
    50701 cttccccgcc cacgccttct ccgcggaggc gtcgacgccg accccggtcg accgcgccca
    50761 ctcccgaagg tcgccgagga tgtcgcgctc ccccttcagg aagacgagct gcatcggggc
    50821 accctcgtag aacgcgacgg cctcctccca tccgcggggc gtcgtgtaga ccgcgaggcg
    50881 aaggcccgcg agccgccgcc ggatctccgc cttcgcctcg ggcggctggg gggacctctc
    50941 gagctccgcg aggaccgact gcatctcgac cttcgcgcgg tcggagacga ccttcgtcat
    51001 ctccggctcg aaccggcctc ccggaggggc cgggaccccc ttcaccggcg cgggctcggc
    51061 gagccagaag agcgtcagga gagcgaggac gggcgccatc gcgcgagagt ttacccgccg
    51121 gccccgccgt cgcgcttctt cacgacgatc tcggaaggct gcccggcgag gtagcgaagg
    51181 gagcactcgg cgaagccctc gcaccggagg cagacgaggt ccttgtcccc cttgaagtcg
    51241 accggcttgc agatcttcac gcccggcacg acgagcttct ggagctcgtt tcggagtccc
    51301 tctccccccg gcggctcgac gcccgagcgc tccgtcgcgc ggagcgcgtc gactcggggg
    51361 aagagctccc ggttccgctt ctcgagcgtc tcgtccttct gctgcacgac cgccgtcttc
    51421 accgcgacga ccgcctcgcg cgccgccttc ctcgccttga agcgcccgtg gtggcgaacc
    51481 cagaagaaga ggaagccggc ccccgcgccg aggacgcgga cgaacgccgg cccggcgccg
    51541 aacgtcacga ggtcgcggaa gaaggccagc cccaggagcg ccgcgctgaa gacggcgatc
    51601 cacttcacct tcaccgggat gacgaagaag acgaggaact gcgcctccgg gaagaggacg
    51661 gcgtaggcga agagcatcga gatcgggacg acgaacgggt cggagacgag cggcgtcccg
    51721 aggagccagg ccgagagcga cgccccgagc gtggcgacga gccagaaggc cgtgtactcc
    51781 gccgtgcccc actcggcctc gagcgcgctc ccgaggatgt agagcgcgaa cgctccgaag
    51841 aagagcgaca ccggtccgcc gtgcaggaac tggaacgtca cgaagcccca cggctgcgag
    51901 agcgcctgct gcggccagaa gccgaagagg ctcgccgtct tctgcgggtc catcgagaaa
    51961 aggaagaaga agacgaagtg gaggccgcag agcggcagcg tgaggccgag cttcggcgcg
    52021 aagaggtcgc ggcccgaggt catccggagc tccttccgaa gcgcttcagc acctcggcgt
    52081 gggcgagcgt gaggaggatg gggcgcccgt gcgggcaggt ccacgggtcg cggcaggagg
    52141 agagatcggc gaggaggcgc gcggcctcct cgggggcgag gcggcggttc acggtgatcg
    52201 ccccgcggca ggcgagcgac gccgcgaggg cgtccttcat ccgcgccgcg gtgtcgcccg
    52261 cccgtccagc gcccgttccg gccgcttcga ggagccggcc gaggaggtcg cggaggaccg
    52321 cgacgacctt gtccgccggc acgtccgcag gcgccgccga gacgacgaag agccgccccg
    52381 cgagctccga gacggcgaat cccgcggccg agagcgtctc gtcggcgcgc cggagcgcct
    52441 cggcctcctc gggcgcggcc tcgaacgaca cgggcgagag gagcgcctgg gacgccagcc
    52501 cctgcccttc gagacagtcg cggatccgct cgaaccggac cctctcgtgc gcgacgtgct
    52561 ggtcgacgag gacgaggccg tcggctccct ccgcgacgag gtacgactcg cggtactggc
    52621 cgaggagccg gagcgtcccg gcgggagacg ccaccgcggc gacggacgag gcgccctccc
    52681 acgagccgcg ggacggctcc gccgggaacg ccgacgccgt gccgcccggc gcgagggccg
    52741 ctccgtccca gagaccgagc gtctccgcga gggcggcggc cgccgggagc gcatcggtcc
    52801 agccggccgc tcgagcggag cccgggaggc cccggccgcc tcccgcgagg accgagccgg
    52861 gcagctccgc cgccgacttg ccctccgtca gcgcggcgcg caggccgcgg tggacgagcg
    52921 cgtggaccgc ggccggatcg aggaagcgga cctcgctctt ctgagggtgc acgttcgggt
    52981 cgacccgctc gggcgggcag gtgacgaaga gcgcccaggc cgggtgccgc tcgccgatga
    53041 gcgcctcctc cgccgcgagg cgtgccgcgt gcgcgaccga tccgtcgcgc gccgcccggc
    53101 cgttcacgaa gagccactgg aacgtccgcg aggcgaacgt cgtctcgggt ggcgagacgg
    53161 cgcccaggag ccgcagtccc gcgtggacgg agtcgacggc gatcacgcgc ggcagcgaac
    53221 cgcgccccat cacttccgcg aagcggtcga gcgcaccggc ggccgatggg agggacagga
    53281 gctcgcgctc cccggcccga agcgtgaacg acacgtcggg gcgcgagagg gcgagcgtcg
    53341 tcagcgcttt cacgacggcc cgctgctcgg cgtcctgcga cttcaggtac ttccggcggg
    53401 ccgggacgtt cccgaagagg tcctccacga cgacgcgcgt ccccttcgtc tgccgcgcgg
    53461 gccgcgcgac gggcgtctcg ccccggtcgg cgacgacctc cgtgccgagg cccgagccgt
    53521 cgggcgacgt cacgaggacg aggcgcgaga cggatgcgat cgacgggagc gcctcgcccc
    53581 ggaagccgag actcccgatc cggccgaggt cttccgccgt ccggaccttc gacgtcgcgt
    53641 gccgctccag cgcgagccgg gcgtcgtccg cgtccatccc gcagccatcg tcggacaccg
    53701 agatccgggc gatgccgccc ccttcgatct cgacgtcgac ccggcgcgcc ccggcgtcga
    53761 gcgcgttctc gacgagctcc ttcacgaccg acgccggccg ctcgacgacc tcgccggcgg
    53821 cgatctggtc gacgaggacg tccggaagga ccgcgatccg cgccatcggc cgcggagagt
    53881 agcaggggga cgatcgaggt actctgcggc catggagcgg tcccggcgcc tctccccggc
    53941 cctcttcgcg ctcgcggcgc tcgtcctcgc gatcctcgtg gcggcgtcgg tcctcgtcgg
    54001 ccgcgtggca cggctcccgc gggagatggc cggggcggtc ccgcacggca tccgcgagac
    54061 cgccgcggcc gtctcggacg gcctcgcgaa gctggccgcg gcgatccgga cgcggagcgt
    54121 gacgacggag ctccggagcg cggtcgccga gctgcgcggg acgaagaagc tccaggtggc
    54181 cgagctgacg cagctcgagg tcgtcgagag gcgcgacgcg acgcgcctcc tgggcgtccc
    54241 tctccccgac gtcgtcgtct cggcgcgcgc gccggtgacg tacgtctaca cgctcgacct
    54301 cgacgcacgc tgggacttcg acctcaccgg caggacggtg agcgtcctgg ccccggacct
    54361 cgtggcgaac gcgcccgcgg tggacgtctc gaagctcgag ctcgtgaaga cggagtcgtc
    54421 gatcctccgg gacgaggacc gcgtcgtcga ggagctgcgc cgctccctca cggacgtctt
    54481 ccgggcccgc gcccggcagc acgtcccgct cgtgcgggag accgcgcgaa aggagtccga
    54541 ggcgttcgtg aggagctggc tccttcgcgc ctacgggctc cccgaggacg tccgggtcgt
    54601 cgtccggttc cacggggagc ccgcgacgat cggcccggcg ggggtcgccc ttccgccccg
    54661 gggctgacgc gaccgaagga cactaatcgg cgacgacggg cgcctcggcc gccggccggc
    54721 ggaaggccag gacgagcgcg ccgagcggga cgacgagaaa gaggagagcc acgagcgcgg
    54781 cgagcgtcgc cagggcgagg cccgtggtcg cggcgacctt cacgaggtcg ttctgccccg
    54841 tgatcgcgac cgtgaggtag tcggtccgcg cggcggcgct gagggcgacg ccgccgaaga
    54901 gcttcacgtt tcgcgcgacg agatagaacg cgaagaaggc ccagacgacg cgctcccaat
    54961 ccggccgctc cgaccgaacg acgcgccgga aggcccaggc cgcgaaggcc gcctccgcca
    55021 ggtggccgcc gaggtcgaag gccgtgacgt gcgccttcgt gaaggcgaag agcgggtaga
    55081 ggagcgccgc ggccgcgaag aggaggttcc agcgcggcgc acggcgatac ctcaccgcga
    55141 ggaagacgag gccgccccag acgagcgccg cgacggcccg gttctgctcg aagacgaccg
    55201 tcatcacgat cgccgggagg gccgtgcagc cgaagaacca ggccacgaac gtgtgcccga
    55261 gctcgtggac gaagaaggag atcccctcgc ccatcacgcg gaacgggaag aaccagccga
    55321 ggaggaccga agggacgaac gcggcgagaa gcgcgcggac tccgggcgcg agggcggccc
    55381 aggcggcggc gacgccctgc gccccgcgcg cctccggctt cgtcggcggc ggagcgggcc
    55441 gcggctcgac cgcgccgggc gcggccggct cgggaaggcc cggcgccgcg atcccggggg
    55501 ctccgtcggc aggctgcggg ggctccgcct ccggcggtgc ggcctcgctc ccggcaggcc
    55561 cgcccgcgtc gccgtactcc acgacgctcc ggacgaggtt ccgctcgtcc cacggggcga
    55621 ggacgtgacg ccagccgccg cccgcgcgcg gctcgacgag gagcacctcc cagaggtcgc
    55681 ccacccagcg gacggccgtt cccggccgga tcggggcccg cgggccgccc tcctcccgtc
    55741 ccgaccatcc cttcggccgg agcgagagga ggacgacggt cccgtcggtg gtcggctcga
    55801 ggcgatcgcg gccggcgggg cgaggcacca cgcggggacg ttaccccgga ccggccgcga
    55861 cgcgaagcct cacttcagcg gcgcgtgaaa ctcacccgag agctgggtcc acttgtcctt
    55921 gacgtcgaac ttccccgaga tcgagtcggc ggtcagcgcc gtgacctcga tcgtccccgt
    55981 gggctgcgag aggctcagcg acgtcccacc ggcgacggcg atcctcacgc tgccggtcat
    56041 ctcggcctgt ccctggtact tcgtcgggtc gtacattccg gcgacgaact ccagcgtctc
    56101 gcccggcgcc acctgcttct gcctcaccag agagagctcg atccgtccct ggcccggctc
    56161 gggcgaggtg cgcttgcggt actcggccat cgtcgtgggg gcgaagtcgt agttcgtgag
    56221 gacgacgacg accgacgcga ccccgatcga ggagtccttg aacgcgatcg ctcgcggcga
    56281 cgcgaagtcg ggggcgttct ccttcggcca ggcgccgttc gtcgacgtca ccgtgacctt
    56341 cgtgaccccc gcgggcgcgg cggccggggc ttccggcgcg ggcgcctcgg cgacgggaac
    56401 ggcctcgggg gccgcgggct tctcggcggg cttgccgcag gcgacgagga gacaggctcc
    56461 cgccaggacg gacagggcga tcgctcgatt cgaggtcatg ggatcctcct gcccccatcc
    56521 tcccgccgga tcgcgcggag agcaattgac gagcgtcacc tcacgttcgt ccaatctgag
    56581 accaattcac aaagaaaccg ccccgccccg gccgaagccg gagcggggcg ggacggtctc
    56641 cgtggggagc ggcctacagg cgcgggagga acgccgtcag gtaggtccag aagttcttca
    56701 ccgacgagat gctcacgcac tcgtcggggg agtgggcgtt ccggatcgtc gggccgaagg
    56761 agatcgtgtc catcccgggg tacttctcac cgatgatccc gcactcgagg ccggcgtgga
    56821 tcgccttgac ctccggctcc ttgtcgaaga gctcggcgtg gacctccttg gcgaggccga
    56881 ggagcggcga ggtcacgttc ggcttccagc cggggtagcc gtcgccctgc ttcacctcga
    56941 agccggcgag ccgcatcgcg gtcgccatca tccggccggc gtccttcttc gccgactcga
    57001 cggagctacg ctggctcgtc gagaaggaga cgacgtcgtc cttcgtctcg acgatcgcga
    57061 ggttcgtcga ggtctggacg aggttcggga tgtcggggga ccagccgagg acgccgtgcg
    57121 gcgccgcatg gatgaagccg acgatccgcg cgaggtcgcc ctcggcgatc gggtcggggg
    57181 cggcgtcgat ccgctcgagg ccgacgttca ccgggttgtc gaagcggccc gcctcggagc
    57241 ggacgtcctc cgcgagcttc gcgagcgccg cgcggacctc gggctccttc accgggtcga
    57301 gccagccgac cgcgaacccc tcgcgggcga tcgcgttccg cttgctgccg ccgtcgagcg
    57361 tcgcggccgc gaagccgaac ccgcccgcct cggtgacggc gcgggcgagg accttcaggg
    57421 cgttcacgcg cccctcgtgg atgtcgcagc cggagtgtcc gccgcggagc ccggtgaccg
    57481 agatccggta cggcgagagt gcggcggacg gcttcgtccg cgcgaccgtc gtcgtggcga
    57541 gcgtgtcgac gccgccggcg cagccgacgt agaccgcccc ctcctcctcg gtgtcgaggt
    57601 tgagcatccg gcggccggtg acgaagccgg gtgcgaggac gttcgccccc gtcatccccg
    57661 tctcttcgtc gatcgtgaag aggcactcga ggggcgggtg ggcgaggtcg ttcgactcga
    57721 ggatcgcgag cgccgcggcg cagccgatcc cgttgtcggc accgagggtc gtcccgtcgg
    57781 ccttgacgac gtctccgtcg acgatgaggc ggatcgggtc cttcgtgaag tcgtgggcgg
    57841 tgcccgagtt cttctcgcag accatgtcga cgtggccctg gaggacgacg accggcttcg
    57901 actcgagccc cttcgtcccg ggcttgctga tgacgacgtt cccgacctgg tcgaccttcg
    57961 cggggaggcc gaggcgggca gcgaccgcca cgacgtgggc cgaggcggcg gcttcgttct
    58021 tggagccgcg ggggatcgcg gcgagggcgg cgaagtggtc ccagagcagc ttcggctcga
    58081 gaccggagag ggggtgggtc atgagggggt ctccttcacg aggccggaga ggccggcccg
    58141 tgatcatagc cggtcggaag gccgtcggcc cctgcgaaag gggcgtccgg ccgccgacca
    58201 gtccggggcg gggagcgctc cgcgcggcac ggcagccgtc ccgtacgatc cgctccggag
    58261 gtcccgatgc gccgtcccgc tctcctcgcc ctgacgctcc tctccgcgct ccccctcgcc
    58321 ggcgccgagc ccgcggcgaa gcgccctctc ggcgtcgacg acatctggtc ggccgtccgc
    58381 gtcggcgcgc cggtcgtctc gcgggacggg acggtcgccg tctacccgcg ttccgtctgg
    58441 gacgcggaga agaaccggct cctctcggac ctctggctcc agccggtcgc gggcgggccg
    58501 ggacgccgtc tcaccgcgca cgaggcgggc gaggcctctc ccgcgatctc gcccgacggg
    58561 gcgcgcgtcg ccttcgtcgc gaagcgcgac ggtgacgagg tcgcgcagct ctacctcctc
    58621 cggctcgacg ggggcgaggc ggaacgcctg accgacctcc ccgccgctcc cggctcaccc
    58681 cgctttacgg ccgacgggaa gcggatcgtc ttctcggcga acgtcctcgg cgagagcctc
    58741 gagacgacga aggcggagct cgcacgtcgg aagaaggagg agaggacgcg cgtcctcgcc
    58801 accttctccg agcggcggct cgtccgatac tgggaccgct ggctcgagca ggacgagtgg
    58861 ccgcacctct tctccgtcga cgtcgcgacg cgagccgtcg ccgacctgac gcccggactg
    58921 aagcggttcg gccgcctcga gggcggcctc gacttcgacg tcgcgcccga cggctccgtc
    58981 gtcttcgccg cgaactcgac cgagcccccg taccgaacgt tgaacaccga cctcttcctc
    59041 gttccggccg gcgggggagc gccgcgaaac ctgacggccg acaagcccgc cgaggactcc
    59101 tcccccgtct tcgcccccga cggccggacg atcgcctacg gacgcgagac gaggggtgac
    59161 ggctggcccg accggacgcg tctcgccctc ctggacgtcg cgagcgggaa gacgaaggcc
    59221 ctcacggaga gctgggaccg cgtcccccag ggctggagct tcacccccga cggtcggacg
    59281 ctcgtcttcc tcgccgagga tcgcgcccga acgcacgtct ggtcggtccc gatctcgggc
    59341 gggacgccgc ggctcgtctg gaaagggggc cgggcgacgg ccgtcgagac cgccggcccg
    59401 tccggcctcg tcttcaccta cacgacgttc cgcaggccgg ccgagctcgc cgcggtcaag
    59461 ctcgacggct ccggcttccg caacctcacg tcgtggaacg acgcgctcgt ggcgcagatc
    59521 gcgttcggcg aggagcgcga ggtcgtcttc aagggggccg gcggagacga cgtccagatg
    59581 ttcgtcgtcc atccgcccgg cttcgagaag gggaagaagt acccgctcgt ccacgtcgtc
    59641 catggcggcc cgatcggcac ctcgggagac gagttccacc tccgctggaa cgcccacgcc
    59701 ttcgcggcac ccggctacgt cgtcgcgctc gtcaacttcc acggctcctc gagcttcggc
    59761 caggcgttcg tcgagtcgat cctgggcgcc ccgggcgaca agccgttcca ggacgtgatg
    59821 gccgcgaccg actacctcgt cgccgagggc tccgtcgacc cgaagcgggt ggcggcggcc
    59881 ggcgggtcgt acggcggcta cctcgtgaac tggatcgccg gccagaccga ccgcttcgcg
    59941 gcgctcgtca gccacgccgg catctattcc ctcctcggcc agttcggctc cgacctcgcc
    60001 tacggccgcc agcattccta cggcgcctat cccttcacga acccctcggc gatcgagcgc
    60061 tggagcccga accgctacgc cgcgagcctg aaaacgccga tgctcgtcgt ccacggcgaa
    60121 aaggacttcc gtgtcccgat cacgcagggg ctcgagctct acggcgtcct gaccgccaag
    60181 ggggtccccg cccgcctcgt cgcctacccc gacgagaacc actgggtcct gaagggaacg
    60241 aacgcgaagc actggtacgg cgaggtcctc gggtggctcg cgaggtggct gaagtagcgc
    60301 ccctcagcgc cgcgccggca tccgttcgac caggagctcg agaacgatcg ccgcccgccc
    60361 gccgtcccag ctcgaatcga tcgtccagcg gagcggcttg cccagcttga gccggaacgt
    60421 ctccgcccgg cgcgccacga gggccgggcc gcccgccgcc ggcgtcctcg tcaccgccgc
    60481 gaagacggcg aagagcaggc tgtcgtcctc cgcgcgaagc tccaggggca ggccgtgaat
    60541 ctcggcgacc tggccgaagg cgtccttcgg tcccgcctcg aagcccgact tcgtgctgaa
    60601 gccggagtcg gagcggaacg cgactctcgg ctcggagagg ctctcctcgg cggcctccgt
    60661 tcgctcgagg agcttcatgg tcaggacgag cggctccgga ttcgggacgc ggaggggctc
    60721 tttcaccacc gctccgggta gcggatctcc gacctccacg agcagccggg gatcgcagcc
    60781 ggcgagccac actctcgtcc cggaggcggc ggcttcgccg aggatcctcc ccttcgcacc
    60841 tccacccgaa ggatcgacgg cggcgagggc gatgccatcc gtcgctcccc ctcgcgcccc
    60901 gaggcggccg agagtcaggg gctcggcgca gcccggcact tcgacgcggc cgacgaagtc
    60961 ggggacgcgg accgcgaccg gggggtcgtc ggcgccgtcg agccagaagc ggagggctcc
    61021 gccgagcggg acgggctcgg acgccggcgc cgcggggacc gtcgccgctc cggtcgcctt
    61081 cgagccggcc ttcgctcgca cccggaggac cttcgacgag accgtgactt cgacgttcgc
    61141 ggcctgtccg atcatctcga gggccttccg gagcggcacg ttcgagatct ccacgctcac
    61201 ccggccgccg acgccggggt ccaggatcgg cgtgacgccg aggaggtcgg cgaacttctc
    61261 gaggacgtcg acgacggagg cgtccttcag ctgcagggcg atggggacgt cgaggcggtc
    61321 ctgcgtgtcc atctcgaccg ggacgggcga gggtgcggct tcgagggcgg gagcgcccac
    61381 gagggccgcc gcgacgagaa cgggaaggat cacagccggt ctcctttcat cggtcgggac
    61441 ggcggaagcg attcggcccg gcggacgagg tcgggtccgg tcaggacgac gcccgtctcg
    61501 tccccctgga ggacgaacac tgtcggcggc tcgggccgtc gggcgagggc gacagagagg
    61561 agcgcgacgg cgacgaggga ggccgcgagg aggcccgagg ccgccgttcg ccgcgcgcga
    61621 cgccacctca ggacggaggc ctgcgcggcg acaagagggt cggcgaggag cgcgtcccgc
    61681 cgcgccgtgg aagagcaggc cgggcacgcg gcgagatggg cggagagggc gctgtcgtcg
    61741 aggtggtcga ggtcggggca gctcatggtt cttcctccag gatcgcggcg atgcggcggc
    61801 ggcctcggca gagccgggcg cggagcgcct cctccccgat gccgagcgcc cgcgcggcct
    61861 ccgccggggt gagcccgtcg tcggccacga ggcgaagcgc gcgcctcgtt ccctcgggga
    61921 gcgactcgag cgccgccgcg gcccgccgcg cgcgttcggc caggagggcg cgcgactcgg
    61981 gatcgaggtc gttcgtcgcc cggagctcct ctccggtcgt cacctcggtc gcggcccgcc
    62041 gccgccgcga ggcgttcgcc ccgatcctcc aggcgatcgc gaagaggagg gaacgcagcg
    62101 ggccgggcgc cggcgcaccg aaccgtcccg gccggctgag gaggaggacg aagaggtcct
    62161 ggacgaggtc gcgggcgtcc tcgccgtggc cccggcgctc cagaaaggcg cggagggagg
    62221 agccgattcg gagcgcctcg tcgacgaccg gcgccgccgg cgctccgcag agctcttcgg
    62281 gaaggtcgct cacgacacgg taatgtcgca cggcgacgtc gcgtgacgcc gccgtgcgaa
    62341 gaatcgccac agtccgggcc gcgaacggcg ggcggcctga ccttccggcc cgccgccccg
    62401 cccctcagac cccggcgttc gccttcagga aggcgatcac cttctccggg tcgtggcgct
    62461 tgccggactc gagcgagccg gtctcctgga ccttcacggc cttgccggtg ccatcgagga
    62521 cgacgaggag cgggacgccc gtcttcgggt cggcgcccca gcccttcgcg agctcgaggt
    62581 tcttcgtcat ccgtccgacg tcgaccttga ccgggatgta cgacgccgcg agcgccgcgg
    62641 agactgcttc gtccttcgtg aagagccgat cgagcgtccg gcaccagcca caccagttcg
    62701 ccccgagcgt gacgaggacc cgcttctttt ccttcttcgc cttcgccacg gccgcggcga
    62761 cgtcggcctt cgcgtcggcc gcctcgtcgt agaccggcgg ggagggaggc ctccggacgg
    62821 cgggcgcctc ggcgattccg ggtccgggct gcttctgggc ggctcctgcg acccccggtc
    62881 ccggctgggc gggagcgggt tgcgcgagga gggcggcggc gagggggagg agggcgagct
    62941 tcatggcagg agagttctac ggacgtccgc ccgtccgcgt ttcgcatgcc gggtcactcg
    63001 ggcgaaggac cgtcgccccg ggggtcgcgt cgggcgaagc cccgcgtcgg gccccgcgtt
    63061 aggtgccgat cacttccaga cggagggacc atgccagaga cccaggcgcc ccgacgggcc
    63121 accgctcgcg ccgccgcgct cctcctctcg ctcgccgcgc ttccgctcgc ggcccagccg
    63181 gtccggaacg gccccctccc cgggccgctc cccctcttcc cgcccgacaa ctggtggaac
    63241 gtcgacgtga gcacggcgcc gcgcgacccg aacgaggcgg cgatcctcgc gttcatcggg
    63301 atgaatcgcg gtctccaccc cgacttcgga ggggacgtct cggcgagcgg ccccgacatc
    63361 tacgggatgc cctacatcgt cgtcccggga tctcagccgc tcgtgccggt cgcgttcgac
    63421 tacgacgacg agagcgaccc gggggcgccc ggccggcccg tcggctaccc gattccgccg
    63481 gaggcgaaaa cggagccgaa gtggctcgag gggggatggc ccgggagctc gggcgcaggc
    63541 ggcgacaagc accttctgat cgtcgaccgc gacaatcggc tcctcttcga gacgtgggac
    63601 acccgttgcg tgccggaggg ctccgcgtcg tgcacctggc gggccggctc cggggccgtc
    63661 ttcccgctcg acgggaacct gcgccgcccc gacggctgga cgagcgccga cgccgccggc
    63721 ctcgcgatcc ttccgggcct cgtgcgctac gacgaggtct tcgggagcga gccgatccgc
    63781 cacgccttcc gcttcaccgt ccgggcgacg aacggctacg tcttccccgc ctcccaccgg
    63841 gccggcacga ctccgggcgc gcctccgttc gggacgcgcc tccggctgaa ggcgtcgcgc
    63901 gacctctcgg agttcccggc gccggtgcgg cggatcttcg aggcgatgaa gacctacggc
    63961 ctgatcctcg ccgacaacgg ctcggacatg tacgtccagg ggacctacga cacgcgctgg
    64021 gacaacgacg tcctgaaccc ggcgttcgcg cgcctgaagg tctcggactt cgaggtcgtg
    64081 cagctcgggt ggcagccggc cggactcccc gtcgacccgt gcgaggcggg gccgggtccg
    64141 tcctgccacg agtggctcct cgcatcgggc gcgcgggtgc gcggcgacgg cggaacgagc
    64201 tggaagaccg acctgacggc cgtgaacggc ggcgagtccg aggcgaccct cttcctccgt
    64261 ttcctcgggc acgacgccga cgggcggaac ggcccggagc gctccttccg cctcgccgcc
    64321 ggccgctcca tgacctggag cgacgttctc ggctccgtct tcgagctcga ggaggcgtgg
    64381 ggtgcgattc agcttcgctc cgcgagcccc gacctcgtcg tcacgagcca gaccttcacc
    64441 cccggcggcg gcggcagcta cgggcagtcg gtccccctct tcgccgagac ggagctgatc
    64501 cggggcagcg cgtcgcggac gatccccgcg atccgcgagg acgcctcctt ccggacgaac
    64561 ctcgtcctcg cgaacggcag cgaggccgcc tgcgacgtcg acgtctcgct cgtctcggag
    64621 ggcgggaccc tcctcggcac gcgccgcgtg ccgctcccgc cgctcgggat gacgcagctg
    64681 acgcgcgtcg tgagggagct cggcgtgacc gccgccgtct ccggggcccg gctcgtcctc
    64741 tcgacgccga ccgcgtcggg cgcgttcgcc gcctacgcct cggtcgtcga cgagaagacg
    64801 ggcgatccga ggacgctcct gccgcggtag cccgcgagct cgcggacggg cgacctcctc
    64861 ggccgctgcc gggaggtcgc cggggctgcc ggggccgccg atctgcagcg tctcgcgccg
    64921 ccctgcaacg tctgcagctt cgccacggcg tcgtcacccc tcctcctctc ccgcaaggcc
    64981 ttgccccggc cggctttcct cgctcccgtg gattgcaggc ctcgcgtggc acggagcttg
    65041 tgagggagag gtgaaagccg gacgccgaag aggccgagcc ggacgacccg aaaggagaca
    65101 cgacgatgaa cggcacccgg aagctctcgg ccctcctcgt gggcaccctc ttcctcgccg
    65161 gcatgctcgc cctcccggcc gccgcgcagc agcagggacc cggcaacggc ggcggcgcga
    65221 aagccggcac gaagcggcag ctcggccccg gcgacgggac gggcgccgcg cccgccccga
    65281 aggacggatc cggctacggc accccctacc gcggcgagaa cccgaacgcc gggcagggcg
    65341 ccggcccggg caacggaacg gggaacggcg gcaacgggcc gaaggacggc acgggctacg
    65401 gaaggaaggc cggccgcggc tcggcgggcg ccgggagctg cgacgggacc ggtccgcggg
    65461 gcggctccgg cggacgcggc tccggcggac ggcgaggcgg gcgcggctga ggtcgcgccg
    65521 gcggcaacgg gcactgcgga atgcctcgcg tccgggcccg cgaggggagg agcgtcacgc
    65581 gctcctcccc gattccgcgc ctcgacccgc ttcggccgga gcgcctcggc cggacgccgc
    65641 gaatcgcgtc gctgctatcg tccttccgat gccgcctccg ccgcaccatc accgctcgca
    65701 gtccgcggtg acgcgcttca tccggccgga ggagatcgac gcgctcctcg agccgtgggt
    65761 ccccgacgcg gaggaccgcc gtttcctgac gcggtgcctc gtcgaggagg ggcccctgca
    65821 ccaccggggg gcgaacttcg tcctcctccg gctcctcggc gagctgtccc gcgccgtcgc
    65881 gtccgggcct gagcgtccgg ctcccggccc cggcctcccc gtaccgatgc gcctcccgcc
    65941 ccacctgcgt ccggacggag aggacgacgc cgtctacccg atccgcctgc cggagcgcgc
    66001 cgtggagcgc ctcgcgccgc gcggcagccg cgacttcgac gccatcgtcg actgcctcgc
    66061 cgacggcccg ccgcagcacg ccgtcgccaa cgtcctcatg gcgacgatcc tggacgacct
    66121 cctccggcgc tccgggcggt gatgagcttc ttcgccccgg gactcgagga cgcgttccgg
    66181 accgcggccg ccgagctcgg gatgtgcagc gcctcccgcc tgttcgtgcg ggaggccgcg
    66241 agcgacggac ccgacgccgt cgcggagctg agcgaccggc tcggccgccc cttccccgtc
    66301 ctcgacgccg tcgccgcggc cgcgctcgag ggggcgccgc tccgcggcgt cgacccgggc
    66361 gaggtcctcg cgacgctcgc gggcctccgg aggctcctcg tcgccggctt cgaagccgac
    66421 ttcctcgacg ccctcgtgcg ggcgctgtcc ggcgaggtcc gcgtcggcct cgtgaggacg
    66481 gcgttcctcg agggcgactg ggagcggatc gccgacaact atgcgggccg cctcgagctg
    66541 gtcgagatgg cggccttcgc cggctgggcc ggcccgagga gcggcctcct cacgttcctc
    66601 tacggcgtcg agggggccgt cacccacgtc gcgccggcct ggctccgcgt ggccggcgac
    66661 gacgtgcggc cgatcttccg cgagctcgtc ggatgggacg tcctcgggcg gccgatgagc
    66721 ctctacccgc gctggctgac ggaggtgccg gccgggagct tcacgaggct cgcatgacgt
    66781 tcgccgccgc cgccacgacc gcgatcctcg ccgcgaccgt cctgctccag gcggcctggc
    66841 ccgcgcggcg cctcgtcctc gtcgcatccg gagcggccct ctcggccctc gtcgtgagcc
    66901 tctcgggcgt ctcgacgacc gggcggctcc tcgctggcgt cccgtgggac gtcctcgtcc
    66961 tcctcgtcgg cctcgggctc gtcaccgagg tcctcgtcgc ctcccgtctc ttcggccttc
    67021 tcgccgtccg ggtcgcacgg gcgacgcgcg ccgcaccggg cctcctcgcg ttcgtcttcg
    67081 tcctcggcat gtacgcggtg agcggagtcg tgaacaacct caccgccctg atgctcctcc
    67141 tccccgtcgt cctgatcctc ttccggctga tgggcgtctc gcagcgctac gtcacctgga
    67201 cgctcgcgac gctcctcgtc gcctgcaacc tcggcggcgc cgcgacgccg atcggcgact
    67261 tcccggcgat cctcctcctc ggccgcgggg cgatggagtt cacggagtac ctggtccggg
    67321 cggcgccgct caccctcgcc gcgctcacgc tcctcctcgc cgccgtcttc ggcctcgccc
    67381 gcccggcccg cggcctcgcg gcgacgcccc tctcggcgcg cctcaccgtc gcgacgctct
    67441 cggcgttcca ccgcggcgtg cggctcgacc ggcgggccct cctctcgggc ctcctgcccc
    67501 tcgcggggat ggtcgccgcc tggctcctcg tcccggcctc gacgggcgtc ggtcccgagc
    67561 tgatctgctg gatcggagcc gccgccgcgc tcctcggcgc cgggcggctc ggagagcgcc
    67621 tcgcccggac gcgcgtcgac gtcgaggcga cgctcttcct cctcttcctc ttcgtgatgg
    67681 tcggcgcggt cggagagacg ggggtcttcc gggacgccgc caggctcctc gccgcgctcc
    67741 cgctgccgcc gacggcgcag gtcgccgtct tcctcgtctc ctcggcgctc ctgacgggcg
    67801 tcttttccgc cggcccgagc atggcggccc tgctgcccgt cgccgaggtc ctcgcggccg
    67861 accacccgcc cgaggcgatc tacgtcggcc tcgccctctc cgtctgcgcc gggagctccc
    67921 tcttcctgac cgccgccacg gccggcccgc tcacgcaggc gctcgtcgag agggccggcc
    67981 tgcgcggcga ggacggggcc ccggtcgacc tcgggttctt ccggttcgcc cccgtgggcc
    68041 tcctcggctt cggcgtcacc ctcgccgtgg cgctcgtcgc gacgctcgcc cgcctgtagg
    68101 cggctcgagc tccgccgaga gcgccgagag gacgcctgcg gccgtctcca ggtcgcccgc
    68161 ggggaatcgc gcgagggtcc ggcggaccgc ttcctcgacg gtcccgcggg tcgcggagga
    68221 gaccttccgc ccggccgccg tcgcgtggag acgcgtcctc ctccggtccg acgggtcctc
    68281 cgaacgctcg acgagcccgg cccgctcgag ccccttcacg atgacggtca ccgtgctcgg
    68341 gtggaggcgc aggaccgccg ccgtctcgcc cggggtgatc cccggaaaac ggccgacgac
    68401 gcgcagcgcg aggcgctgcg gcccggtcac gccgaccgac tccgccatcc gccgcgaggt
    68461 cgtctgaagc gcgtggtcga gccgccagag gagcttcatg aaatcgagcg cctcgccgag
    68521 ggacgcttcc ctcgcgtgca ccgggcccgt cgtcctcccc atctctcctc cgcgcggcgg
    68581 cgagccgcgc ctccatttca tcacatccga atcgtttgga gtcaaatatt ttgacctcaa
    68641 actattttcc ggtaggatcc ccccgtgacg tcgtcccgga accccgcccg tcccccgtcc
    68701 tcttcccgcg tcgccgtctt cggcgaggcg ctcgtcgacg agctcccctc gggcgggcgg
    68761 ccggggggag cccccctgaa cgtcgccgct cacgccgcgg ccttcggcct ctcgccgctc
    68821 ctgctgaccc ggctcggatc ggacccggcc ggcgaggcgc tccgggccgc gatggcgtcg
    68881 cggggcatcc ccgccgacgg ggtgcagtcc gaccccgcgc ggccgacggg acgcgccgtc
    68941 gtgaccctca ggggcggcgc cccccgcttc gagatccccg ccggcgccgc cttcgacgcg
    69001 atcgacggca gcgccgccgc ccgcgtcgtg cacgaggcgc ggccgtcggt cgtcgccttc
    69061 ggcacgctcg ccctccgcga gccggcctcg cgcgaggcgc tccgcgaggt cctcgacgcc
    69121 tcgcccgcga tccggctcgt cgacctgaac cttcgcgccc cctggtatga cggcgcggtc
    69181 gtgacccgcg ccctctcggc ggcgcacgtc gtgaaagtga gcgacgagga gctcgacgag
    69241 ctgcgccgcc tcctctgcct gacgctccgg gagccggagg cgctcgccgc cgacgtcgtc
    69301 cgccggttcc gcctgaaggc gctctacgtc acgcgcggcg cgcggggagc cgccgtctcc
    69361 tggcgctcgg gcgacgtcgt ccaccgggcc gaggccgacg ccgccggctc gcaggacgag
    69421 gtcgtcgaca ccgtcggggc cggcgacgcc ttcacggccg tcgtcctcct cggcctcgtc
    69481 cggcactgga cggcggagga gagcctccgt cgaggcgtgg agctcgccgg ggccgtcacg
    69541 cggatcgccg gcgcgacgcc ggaggagccg tcgttctacg cgccgttcct cgcgcagtgg
    69601 tctgaggcga agtgaacggc tccgccgacg gcctctacct cttccacctg agcctccacg
    69661 ggctcctgcg cggcacgagt ccggagctcg ggcgcgatcc cgacaccggc ggccagatca
    69721 cctacgtcct cgagctcgtc cgcgcgctcg ggcgctcgcc gcgcgtgtcc cgggtcgacc
    69781 tcgtgacccg gctcgtcgag gaccccgcgg tcggacccga ctacgccgtc ccgatcgagc
    69841 ccctcggcga gagcgtgcgc atcgtgcgcc tcccgttcgg cccgaagcgg tacctgcgca
    69901 aggagctcct ctggccccac ctccctgcgc tcgtcgaccg gtgcgtcgag cacctgcgag
    69961 cgcaggagcg gctcccgcac ctgatccaca cccactacgc cgacgccggc tacgtcggcg
    70021 tgcggctctc ccagctgctc gggatcccgc tcgtccacac ggggcactcc ctcggccgga
    70081 cgaagcgctc ccgctacctc gccgcgggac gcgccgaggc gagcctcgag cgccccttcc
    70141 gcttctcgca gcggatcccg gcagaggagg aggtcctcga gaacgcgcgg ctcgtcctgg
    70201 cgagcacgcg gcaggaggtc gacgagcagt ggggcgcgta cgagaacttc cgccccggcc
    70261 gtttcgcggt cgtccctccc ggcgtcgaca cctcgcgctt ctctccgccc tccccgttcc
    70321 gcgcgcggac gtcgttcccc gagatcgacc ggtggctccg ggagccgcac cggccgctcg
    70381 tcctcgccat cagccgcccc tcgccgaaga agaacctccc cgcgctcgtc gaggcgttcg
    70441 ggcgcgatcc gcgcctgcgc gagacggcga acctcgcgat cttcgccggc gtgcgggagg
    70501 acgtcgcccg gagcgacgaa gaggcgaagg ccgtctacac ggagctcctc ctcgcggcgg
    70561 accggcacga cctctggggc tcaatcgcgc tcccgaagaa gcactccccg gaggaggtcc
    70621 cggacctcta ccggctcgcg gcgcggcgac gcggcgtctt cgtgaacgcg gcgctgatcg
    70681 agccgttcgg gctcacgctc ctcgaggcgg ccgcgagcgg cctccccgtc gtcgcgaccg
    70741 aggacggcgg gccgcgcgac atcctcgcga actgccggaa cggcctcctc gtcgacccgc
    70801 tcgacgtcga cgccctctcg cgggcgctcg tcgaggcgct cggcgacgcc ggccgctggt
    70861 cgagctggtc gcgcaacggc ctccggcgcg tccgcgacac ctactcctgg gacggccacg
    70921 tctcgcgtta cctcgcgctc tgcgagcggc tcctgcgccg cgaccgaaag gctctccggc
    70981 ggcggcacgc cgccctcaag gcgacccctc tcgcccacct cgcggacgcg acccacctcc
    71041 tcgtcaccga catcgacgac acgctgatcg gcgacgccgc ctcgctcgcc cggctcctcg
    71101 agtggcgccg cgcgcacgcc ggccgcgtcg cgttcggcgt ggcgacgggg cgctcgttcc
    71161 cgaggacgct ggaggtcctc gcgaagtggc gcgtcccgct ccccgacgtc ctcgtcacct
    71221 cggtcggcac cgagatccgc ttcgccccgg agctccggct cgaccgggcg tgggagggtc
    71281 acatccggca cggctggagg cgggacgagg tcgttcgcgc gctcgccggc gcgacgagcc
    71341 tccgcaagca gccggaggag aacgacggcc cctacaaggc gagctgggac gtcgtccccg
    71401 ggcggaacgt cgacctcgcg gcgatccagc gcctcctccg ggcgcgcggc ctcgcggccc
    71461 gcctcgtctt ctcgcagggg aagttcctcg acgtcctgcc ggtccgcgcc tcgaaggggc
    71521 tcgccgtccg cttcctcgcc ctccgcctcg gcctcccgct cgagtcgttc ctcgtcgcgg
    71581 gggactcggg gaacgacctc gagatgctcg tcggcgacac ccgcgcggtc gtggtcggca
    71641 accacaagcc ggagctcgag gagctccggg ggcgcgacgg ggtctacttc gcgaccgctc
    71701 cctacgccgg cgggatcctg gaggggatcg cgcactactc cttcgcgacg gaggcgccat
    71761 gctgacccgc gtccacgact tcgccgccgc ccaccccgac gtcctcgccg tcctcgtgag
    71821 ccgatggctc gcggaggggc gcgacatcct cctccgatcc gacctcgtcc gcgggtggga
    71881 ggagacccgg gccgtctgcg accgcgtcga cctcgccgcc tcgccgctcg ccgaggcggc
    71941 cgcggcgctc cacgaggggg tcttccgcgc cccctggatc gccctctcgt tccgcacggg
    72001 gcccggccgc tggcgctacg cgcgcctcca cgccgaccgc ctcgtccccg aggagatcac
    72061 ggtctccgag ttccttcgct tcaaggaagg gatcgtttcc tacgggcgcg gcgaggagcc
    72121 gctcgagctc gacttcggcc ccttcggccg ggagttcccg cgtctcacgg agccgcgctc
    72181 gatcgggcag gggctctcct tcctgaaccg ccacctcgcc agccggctct tccgcgacgg
    72241 cggctcgggg gcggaccgga tccacacctt cctcggcctc cacgccctcg aggggcggac
    72301 gctcctcctc cgcgcgtcgt tcgccgacac agcggcgctc cgggccgccc tgcgcaaggc
    72361 ccacgacctc ctcgccgcgg cggagcccgc gactccgtac cgcgagctcg aggagcgcct
    72421 cgccggcctc ggcttcgccc ccggctgggg ggcgaccgcc ggacgcgccg ccgagacgat
    72481 gagcctcctg atcgacctcc tcgaggcgcc gtccgcgacg ctcctcgagg cgttcctcgc
    72541 ccgcgtcccg atgatctcgc gcgtcctcgt cctctccccg cacggctggt tcgggcagtc
    72601 gggcgtcctc ggcctccccg acaccggcgg ccaggtcgtc tacatcctcg accaggtccg
    72661 ggccctcgag cgcgagatgc gcgagcgcct cgccgagcaa gggatcgacg tggagccgcg
    72721 gatcgtcgtc gtcacgcgcc tcatccccga cgccgggacg accggctgcg acgagcggct
    72781 cgagcggatc gacggctgcc ggagcgccgt catcctgcgc gtcccgttcc gcgaggcgga
    72841 cgggagcgtc gtccgcccgt tcctctcccg cttcaaggtc tggccctacc tccaccgctt
    72901 ctcccacgag gtggagacgg aggcggcggc ggagctcggc gggaggcccg acctgatcct
    72961 gggcaactac tccgacggga acctcgtcgc ggccctgatc ggcatgcgcc tcggcgtgac
    73021 gcacgcgacg atcgcgcacg cgctcgagaa gacgaagtac ctcctctcgg cggtccactg
    73081 gaaggagatg gaggaggagt acgcgttctc cgttcagtac acggccgacc tcttcgcgat
    73141 gaacagcgcc gacttcatcg tcacgagcac ctaccaggag atcgccggga cgaaggaggc
    73201 ggtcgggcag tacgagagct actcttcgtt caccatgccg ggcctctacc gggtcgtgaa
    73261 cggagtcgac gtcttcgacc cccgcttcaa catcgtctcc cccggcgccg acgcggacgt
    73321 ctacttctcg ttccgcgatc gggagcggcg cctcgccgtc ctccaccccg cgatcgagga
    73381 gctcgtcctc ggcgagccgg ggcccgaggc gcgcggccgg ctcgtcgacc cgggcaagcc
    73441 gatcgtcttc acgatggccc gcctcgaccg gatcaagaac ctcgccggcc tcgtcggctg
    73501 gtgggcgcac gacgagcgac tccggtccct cgcgaacctc gtcgtcgtcg gcgggcacgt
    73561 cgaccccgac cgctgcgccg acggcgagga gcacgagcag gcccggctcc tccacgaccg
    73621 gatgacgcgc cacgccctcg acggcgaggt ccgctggatc gggaagcggc tggaccgcca
    73681 cctgacgggc gagctctacc ggtgggtcgc cgaccggcgc ggcctcttcg tccagccggc
    73741 gctcttcgaa gcgttcgggc tgacggtcgt cgaggcgatg gtctcggggc tcccggtctt
    73801 cgcgacctgc cacggcggcc cgtccgagat catcgaggac tcccgctccg gcttccacgt
    73861 cgacccgaac gacggcgcgg cggcgacggc gcggatcgcc gccttcctcg agcgctgcgc
    73921 ggccgacccc tccgcgtggg accgcgtctc cgaggcggcc gcggcgcgcg tcgccgcccg
    73981 ttacacgtgg cgacgctacg cggagcggat catgacgttc tcgcggattt acgggttctg
    74041 gaagttcgtc tcggggctcg agcggcagga gacggcgcgc tacctccaga cgctcgatca
    74101 cctcctcctc cgtccccgcg ccgcggccgt cggcggaggc cccggctgag cgggattccc
    74161 ccgtccggcg gcaccgggcc gcgcgcgtgg ctggaggcgt tcctcgctct ccttcggccg
    74221 cgctcgcgcc agggcgtcct cgtcacggtc gtcctgatcg gcgcggggtg cggcctcgtc
    74281 gcggtcgcct tccacggcgc catcgagctc cttcgcgccc tcttcgtcgg gacggcgctc
    74341 cggcaggcga gccccctcgc acgcggggcc ctcctcgtgg cgatcccggc ggccgccggg
    74401 gcgctcctcg cctacctcgt cccgctcttc gctccgcgcg cgggcggggg gctcgccctc
    74461 gtccggaagg cgtatgcggg cgacccgacg accctcctcg acccgcgcac cctcgtcgga
    74521 gccttcggcg cgaactcgct ctcgctcggc gccggcgtgc cgctcggccc cgagggcccg
    74581 acggtcgtcc tgacgagcgg cttcgccgcg ttcgccgcgc gctggctccg gctcccgggg
    74641 cggttcgtcc gcgggatggt cccggtcggg acggccgcgg ggatcgcggc catcttcaac
    74701 gcgccgatca ccggggtcgt tttcgcgctc gaggaggtga tcggcgccgc ctcgcgcggc
    74761 gtcctcggag gctcgctcct cgccgcggtc gccgcggccg tcgtcgagcg gacgttcctg
    74821 ggcggcggcc gcctcctccc cgcggctccc gcccactggg gcgacctgcg cgagctcctc
    74881 ggcttcgccg tcctcggcct cctctgcggc gccgcggccg gctggctgcc gcgcttcgtc
    74941 ccgatcctcg gtcgaggcct cgaggcgctc ctcgtgcgga ccgggcgcca cgccctcgtg
    75001 gcgcgcggcg ccctcgccgg ggccgtcgcc ggcctcctcg gcgtcgtcgc gcccgagacg
    75061 ctcggcatcg gctacccggc cgtctcggag tggctgacgg gaggaggcag cgggccgcag
    75121 gccgcgatcg cgttcgcggc gaagctcgcc ggcgtctccc tcgccctcgc cgcgccgctc
    75181 gtcggcggcg tcttcgcgcc gtcgctcttc ctcggcgcct cgctcggcgc cgccgcgggg
    75241 cacctcggcc agcacctctt cccgctcgcg cacatcgagc cgggggcctg ggcgctcgtc
    75301 gggatggggg cgttcttcgc ggggttcctc cggacgccga tcgcctcggt cctcatcgtc
    75361 ttcgaggtga ccggcgacta cggcctcgtc ctgccccaga tgctgaccgt cgtcctcgcc
    75421 tcggtcgtcg cgcggaggat ctcgcgcgag acgctcgtcg agcgcgcgct cgcccccgcc
    75481 gaacggcgcc ccgggggccg ccacgatccg ctcgccacgc tccgggtcgc cgacgtcatg
    75541 acgaccgacc tccggtcccc gcgcgcctcg atgacgatcc tcgaggcggc ccgcgtcgcc
    75601 gccggcacgc gccacgagca gtaccccctc gtcgacgacg ccggcctcct cgccggcgtc
    75661 ctcttcgcgc gcgacctcga ccgggcgatc ctcgagaacc ggaaggaggc gaccgtcggg
    75721 agcctcgccc tcccgccggt cctcgtcctg acgccggagg tgacgctcga cgacgcggcg
    75781 ctccggctcg cgcgcgcggg cgagtcgcgc gccccggtcg tcgcgtcgga ggaggagccg
    75841 cggctcgtcg ggttcctctc gtcgtccgac ctcgtccggg cgcgcctcaa ggtccacgag
    75901 gaggagtcgg gcccctcgat ggcggacctc ggatgagccg ccgcggggcc ggcgcctcgg
    75961 ccgtcctcgc gctcctcctc gcggcctccg cgtgccggcc gggcgggaac gccgcgcccg
    76021 gcgacggcgt cgtcctccgg atgctcgtcc ccgcgaacga gcgccccttc tggctccctc
    76081 tcgcggagac gttcgcgaag gcccggcccg gcgtccgcct cgagctcgag gaggggccgg
    76141 tcccgaccga cctgcgcgag agcctgacga cctcggcgct cctctcgcgc gacggcgcct
    76201 acgacctcgt ctacctcgac gtcacctgga cctcgaagct cgccgcggcc ggctggctcc
    76261 tcccgctcga cgacgccttc ggcgaggagg agcgcgccgc gctcctgccg caggcgctta
    76321 cggccggcgt cttccgcggg cgcctctacc gcgtgccgtt ccggaccgac gtcggcctcc
    76381 tctactaccg tgccgacctc ctcgcgaagg cggggatcgc tccgccgcgc acgttcgccg
    76441 agctggaagc cgcagcccga cggctccagt cgccgccgga tcgctggggg ttcctctggc
    76501 agggggcgca gtacgagggg ctcgtctgct tcttcctcga ggtcctccac ggccacggcg
    76561 ggagctggat cgacccggag acgctcgagg tcggcctcga ccgccccgag gccgaggcgg
    76621 cgctcgcctt cctcgtccgc gcgcgcggcg cgggcggcat cagccccccc ggcgtcacgg
    76681 cctaccgcga ggaggagacg cggcacctct tccagtcggg gcgggccgtc ttcctccgga
    76741 gctggcccta cgtcttccgg ctcgcgcaac gggaggagtc ggccgtcgcc gggaggatcg
    76801 gcgtcctccc cgtcccgacc ctctcgggcg cgcccggctc cgggacgctc ggcggctggg
    76861 ggctcgccgt cgcgcgcgcc tcgcgccggc cggaggaggc gatcgcgttc gtgagacacg
    76921 cgacgacgct tccgagccag cggctcttct gcgcgccgac cggcttcgcc cccgcgcgtc
    76981 gcgacgcctg ggccgatccc gagctcctcg ccgcgaaccc gttcctcgcc gagctcggga
    77041 ggatccacgc ttccgccacc gcgcgccccg tcgtcgcccg ctacgcgcag gcttccgacg
    77101 tcctccagcg gcacctcacc gcggcgctcg ccggcctctc cccgccgcgc gcggcgctcg
    77161 ccgccgccgc aagggagacg cgcgccctcc tcggggaggc gccgtgagca ccgtcctcgc
    77221 gcgccgcgcg cggcgcgagc gggcgctcct cctctcgccc gtcctcctcc tcctcgcgct
    77281 cgtcgtcgcc gtgccggcgc tccgcgtcct tctcctcggc ttcacccgga cggagctcgc
    77341 ggacggcgtc gtctcctcct gggccggcct cgcgacgcac gcgcggaccc tcggcgacgg
    77401 ccgcttcctc tcggccctcc ggaacacggc cgtcttcacg ttcctctccg tcctcctcga
    77461 ggtgacgctc gggacggcgc tcgccctcct cctcgaccgc tccttccgcg gccggggcgt
    77521 gctgcgcgcc gtcgtcctcg tcccgtgggc cctcccgacg tccgtgatgg cgctcgcctg
    77581 ggcctggatc ttccacgaca cgttcgggat ggcgaacgac ctcctcctcc gcatcggcct
    77641 cgtcgagacg cccgtcgcct ggctcgcgca gcccggcacg gcgatggccg ccctcgtcgt
    77701 cgccgacgcc tggaagacga cccccttcgt cgcgctcatc atcctcgcgg ggctccaggg
    77761 gatcccgcgc gaggtcgtcg aggccgcgcg cgtcgacggc gcgggggcct tcgcccgctt
    77821 ccggcggatc accctcccgc tcctcgtccc gtcgctcgtc gtcgcgaccc tcttccgctc
    77881 gatccaggcc tggggcgcct tcgacgtcgt ctgggtgatg acgggaggcg gccccggcgg
    77941 ctccaccgag acgctctcgc tctacgccta ccagagctgg ttccgctacc tcgacttcgg
    78001 ctacggcgcg gcgatcgccg tgcaggggat gctcgtcgcg ctcctcctcg cggccctcgt
    78061 cgtgaggctc gcctcgcgga gggaggaggc gtgaggcgct ccgtcgccgc ggcgttcctc
    78121 gcgctcggcg tctgcctcgc ggcggcctgg agcctcctcc cggccgggtg gcaggcgctg
    78181 acggccgtga agcccgaggc gcagatcacc gcctcgccgg ccgtctgggt cccgagccct
    78241 ccgacgacgg agcacgtccg ctcgctcctc gcgagaaagc cgttcgaccg ctacctcgcc
    78301 aacagcgccc tcgtctccct cgcggcgacg ctcctcgccc tcgcggccgc cgcccccgcc
    78361 gcttccgcgc tctgccggat gcgcccccgc gcgcggagcc gctggctcct cggcctcctc
    78421 ctcgtctctc tcttcccgcc gatcctcctc ctcttcccgc tctacgaggg ggtgaggctc
    78481 ctcggcctcg tcaacaaccc gctcgcgctc gtcctcccct acgccgcgtt cggcgtcccg
    78541 ctcgcgacgt gggtcctcga ggcgggcttc cgcgagctcc cgaaagaggt cgaggaggcg
    78601 gcgacgctcg acgggctcgg gccgctcggg cggctcctgc gcgtccagct tccgctcgcg
    78661 gcgccctcgg tcgcggcggc cggcctcctc accttcatcg cgtcgtggaa cgagttcctc
    78721 ctcgcgctct ccttcacgac gcgggagtcg agtcgcaccg tcaccgccgg catcgcgagc
    78781 gtgtcgggcg cctcgcttta cgaggtgccg tgggggccgc tctcggccgc cgtcgtcctc
    78841 gcgacgctcc cggtcgtcct cctcgtcctc ctcttcgaaa ggaggatctc cggcgggttg
    78901 accggaggcg cggtgaaggg ctgaacgcga cgaggcccgc cggggtctcc cccggcgggc
    78961 ctcgctggaa tcctcggccg gagcctggcg ctcccggtgc ggggcgcgcc aggcctccgc
    79021 cgcgtcagtc cttgtacttg acgacgagga acttctcgcc cgaccagaac gcggcggcgt
    79081 cgacgatctt cagcgtcgtg tgcgtctcgt cggcggcgaa gagctcgtag ctccccttcg
    79141 ggtgcgcgga gatgacctgg accttcttga gcggcgcgtc gatcgcgaag gcctgctcgg
    79201 ccgtgatgtc gatctcgcgg aagatctcgg ggtcgaactt cccggtctgg acccagctcc
    79261 cgcgcatccc gaggaaggcc cccttcttct cgatgacccc cttggcctcg agctccttct
    79321 gcccggcgat cgccacgtgc gcggtgttga tcaccttggt ctgcccctcg atgacggtct
    79381 ccttctccgc gatcgtcgcc gtctgctcct cgacggtctg cgtgaggccg aggaccttcg
    79441 tctcgagctc ggtgatgagc gtcgtcttct cctcgagcga ggccttcatc tcgccgatga
    79501 ggcgctccat cgaggtcacc tgcccctcca gctccacgac cttggcgccg gaggccttcg
    79561 ccttcgcgag ggcgtccttc ttctcctgct cgaggcgggc gagcttctgg aggttctcct
    79621 ggatcgcctt cttgatcgcg tcgatctcgg tcttcagctc gtcgcgctgc gtggagcgcg
    79681 gcttcccttc ctggacgacc tcgatcgagc tcttcacgat ctggagctcc ttggcccgga
    79741 gctcctcgag gctcttggcg acctcgtcga gcgtcgcctg catctcggcg ctctcgacgc
    79801 cagccgaggt gagctggccc tcgagctgct gcttctcctg cgagagcttc tggttgtcgg
    79861 tgcggagctg ctcgatctcc ttcttccagc cgcagccgcc cagcgcgagg gcggcggcaa
    79921 ggaccccggc gaaggcgacc cgcgtcgcga tgaaccgatg ggacatggtt ttcctcctct
    79981 gagagtgcga tcgagttcgt tggatgactc tcgagattac ctcggagcgg gccggcggga
    80041 gtcaacttcc tgccccggcc ccggaggggc gcccgccgcg gcccggcctc gcccgggagg
    80101 ccggggtgag atgatccctg gccgaaaagg gagcagagac cgatgtcgaa cgtgcggacg
    80161 aagcgcggaa cgatcgcgat tctgaccggg ggaggagacg tcccgggcct gaacccggcc
    80221 atccgggcca tcaccgtccg ggcgctccgg gacggcttcc gggtcctcgg catccgccgg
    80281 ggctgggccg gcctgatcga gctggacggg ggaacgggga ggcccgacgg cgactgcgtc
    80341 gtcgagctct ccgaggagat cgtcaacaag atcgggcgga cgggcgggac gttcctccac
    80401 acgtcccgga cccgcccgtc gcacgtcccg cgggcgaacg tcccggagca cctgcgagac
    80461 cgctactccg gcgagatgaa cgacctcacc cccgaggtcc tgtcgaacgt cgggaagctc
    80521 ggcatcgacg tcctgatccc gatcggaggg gacgacacgc tctcgtacgg cgtccgcctc
    80581 cacaaggagg gggtgaaggt cgtcgcactc ccgaagacga tggacaacga cgtccccggg
    80641 accgactact gcatcggctt ctcgacctgc gtcacgagga cgatcgagat gacgcacgcc
    80701 ctccggacct cggccgggag ccacgagcgc ttcctcgtca tcgaggtctt cggccgctac
    80761 gccggcttca ccgccctcct cccgacgatg gcgggtgccg cgaaccgctg cgtcatcccc
    80821 gagcacccgt tcgacgtcga ccggctgacg gagctcctgg tcgccgaccg gacgacgaac
    80881 ccgagccgct acagcgtcgt cctcgtctcc gagggggcca agttcgccgg gatggacgag
    80941 atggtctact cggaccagga gcaggacgcc tacggccaca agaagctcgg aggcgtcggc
    81001 gacctcgtcg ccgagaagct ccggcagctc tcgccccgcc acgccggagg gaggcgggtc
    81061 aacacgatca gccagcgact cggctacctc gtccgctgcg gcgaccccga cgcgctcgac
    81121 tcgatcgtcc cgatggcgtt cggcaacctc gcgatggacc tgatcctccg ggggagcttc
    81181 gggcggctcg tctgcgtgag gaacggccgc tacgacgacg tcccggtcga cgtcgtgacg
    81241 agccgaaaga agtcggtcga cgtcgcgaaa cactacgtcg ccgaccgcct ccgcccccgc
    81301 tacgaggact tcgggaacaa gccggtcttc atcatgacga gcgactactg aggcgtgtcc
    81361 ggccctcccg cgcgaatccg tcgtaacctc cgggcggagc cgagcacgtg atacgcctct
    81421 acggattcgt cgacgggacc ctccgcgccc ttcacctcgc gccgatgggc gaggcgagcg
    81481 gccccgagtg ggacgcggtc gcctgggtcg acctcctcga gcccgagccc gaggagaagt
    81541 cctgggtgga gcgggtctgg gggatcgagg tcccctcgcg ctccgagatg cgcgagctcg
    81601 aggcgagctc ccgctgctac caggaggccg acagcctcca cctgctcacc gccttcatcg
    81661 cgagcgagcc gggccccgcg cacacgatcg acgtctggtt caacctccac cgcggtcgcc
    81721 tcttcaccgt ccggcccgag aagctcccgc cgttccgcct cttccgcctc cgcgccacga
    81781 gccagcccga ggaggtccgg gagccgatcg acatcccgct cggaatcatc gaggccgaga
    81841 tcgacgtcct ctccgacgtc ctcgaggcgg cctacgcccg gctggagcgg gtgagccgct
    81901 ccgtcctgac cgagggagcg cgcgacctcg aggacctcct cgagaagctc gcggccgagg
    81961 aggacctcca gggcaagacc cgcctcgtcc tcctcgacct ccgccgcagc ctgaccttcc
    82021 tcctccgcta cgtcgtcggg tcgaccccgc gcgcgaacgt gatcaagggg gcgctccgcg
    82081 acatcgagtc gctcaccagc cactcgggct tcgtcttcga gaaggtgaag ttcctgatgg
    82141 acgcgacgat gggcttcatc aacctcgagc agaacaagat catcaagatc ttctcgatcg
    82201 cggcggtggt cttcctgccg ccgacgctcg tcgcgagcat ctacggcatg aacttccgcg
    82261 tcctccccga gctcgagtgg ctctggggct accccttcgc cctcgggctg atggtcctct
    82321 ccgggatcgc cccctactgg ctcttcaagc gcaagggctg gctgtagccg ctcacccctg
    82381 ccgcttcagc tccgccacga gccggcgcag cgtggcgtcg ccgtcgtcgg gagggagcgg
    82441 gcaggagccg gcgccccggt gcccgccgcc tccgaaaagc gacatcgtgt ggccgacgtc
    82501 gctccagctc gaccgctcga agatgttgtg ccccacggtc gccatgacga cgtccctccg
    82561 cggcccccac tggagcctca ccgagacggt cgcctccggg aagagcgtgt agacgaggaa
    82621 gcggttcccg atcgggatcg gggagaggtc ccggaagtcg gtcacgacga cgttcgcctc
    82681 gagccgcgag gaggcgagga gcgccttgcg gaactcctcg tcggcctccc gcatcttcgc
    82741 gacgcgctcc gtcacctgcg gcgagcggag gacctcgtcg agcggcatcg agcggagtgc
    82801 catcccgagg aagaggaagt agtccctgaa gccgctgagg ccggtcctcg agtcgatcgt
    82861 gaagccgagg aggatgaccc gttccgggtc gaggacgtcg cggcgggtca ggttcgccga
    82921 gtcgacccgg tcggtcccgg ccacgagctc cccgtatcgc tcgaagagcc gccacccctt
    82981 ccgctcgcag tagtagtcgt ggacgaggcg ggccgtcgac ggggcgaggc cgtatcgccc
    83041 ctggaaggcc gcctcgggcc gcgcgttcgc gtcgacgagc tcgtggtggt cgaaccacat
    83101 cgagcagctc gggtggaacg ggaggttcgc gaggacgtcc ccgtcgcgca cctcgaaccg
    83161 cccg
//
LOCUS       DAOONL010000004        75917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_183_length_75917_cov_18.576244, whole genome shotgun sequence.
ACCESSION   DAOONL010000004 DAOONL010000000
VERSION     DAOONL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 75917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75917
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_75917_cov_18.576244"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="PLP50_01245"
     CDS             complement(<1..1729)
                     /locus_tag="PLP50_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA50199.1"
                     /translation="MTSIYFGEEHESFRRSVRQFLEGEVAPRAREWEEARRIPRDVFR
                     RMGDLGFLGVLAPEELGGADGSVFHAIALLEELPRSRMGGFVAAVSVQQFIATGAIAL
                     HGTEEQKHRWLAPSVAGTKVGAIAISEPDTGSDVAAIRTTARRDGDAWVIDGAKTWIT
                     NGVEGDFVVVACRTAADAGAGGISLLVVEADTPGFSRSRLRKMGWHCSDTAELTFTDV
                     RVPLGNLIGAENEGFFLIMETFVLERLVTAATSVGSARLALEETRKYVLSREAFGKPI
                     GKFQAIRHRLADLHAELEAAAQLVYHAAWLHERGEDPVAEACMAKLLATELSKRTADE
                     CLQFFGGYGTVEEYPMERFFRDARFGTIVAGTSEIMREVIAKSAIDGVTFPGRAERRD
                     AQAEAGAEGAPAGGDSEADADTDAFAGDLVPDLFVADVTETLDAVLAEVPPPPAGSDP
                     GRAAAEVPSPASPEPPAPAVEATVPSFVPPPPAPPPADVEVTEPAYFPEPSAPPSPAR
                     PTPEIGSTSIAMKLPVPRGVEELFETLPLRFRSDRAQGWNARFHFRFRDAARSDWTVV
                     VEDGFCRTVE"
     gene            1898..2302
                     /locus_tag="PLP50_01250"
     CDS             1898..2302
                     /locus_tag="PLP50_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HPA50200.1"
                     /translation="MSSRRDFLGSAWAAAGVAAAAAGTAVLGRALSGSPEPERYVRLP
                     AELLARAEKEGGLVLEGLHVRGPAASPVVLDLTCTHLRCRVAPIEGGGFACPCHASRF
                     DGEGHPTSGPAKRPLSRPRLSPDGAGFVARLS"
     gene            2299..3120
                     /locus_tag="PLP50_01255"
     CDS             2299..3120
                     /locus_tag="PLP50_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50201.1"
                     /translation="MILGRLTFGLLAIAVLSGLALAPFWSAARPLESLEALIGATPLG
                     FWLRGVHAFSAWGFLLGTVAHLAEVLAKRTERRLRPIVWWRSVALLPIAVLGLLGGFL
                     MRGDAEAQAALAVFREVSATVPIAGRALGALLLGVPGSDLAPVALHHAGTFTLLAWLL
                     TAEHGGTPFPDLRATVLAALGSFAAAGLFPVPLGPPVAPDGDLLLGPWYLLGLQGALL
                     DLPVAAGWLLPLALLLAVGLARHVDGRARAALLALAAAWGAGYLALTLRLLLAGR"
     gene            3120..4127
                     /locus_tag="PLP50_01260"
     CDS             3120..4127
                     /locus_tag="PLP50_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50202.1"
                     /translation="MFRAQALLLALLLALAGLLGALGRERQRAAAVGRGPDRAGGKVE
                     RCVTCHVRPEEDPGGAHARAAVGCSSCHLGNPLAFDKTRAHAGMEPEPGAHRSVTRTC
                     GRSGCHVRNVLGERERAIHGNGDGCVACHVARRLPGSVPRPHPGVDARVTDDRCLACH
                     APPAGTHGELAETSPAGSGSASAPADAPSACRAGADGHRFAGLSCIDCHLHAELMGDG
                     AAKETSRVEITCEACHGPVREGGETTWSEIRDPVSRDLLRQRGESREPSERVRLGRRG
                     TPLWNLRPSDDGWTTTRKGHGVALATPSTPVDARHDLRDGGRPACVSCHAVRAPVIPA
                     L"
     gene            complement(4214..4720)
                     /locus_tag="PLP50_01265"
     CDS             complement(4214..4720)
                     /locus_tag="PLP50_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50203.1"
                     /translation="MPFDRRWLLTAAGSAFLASLAEEGLGAPPPNGAQATGDLAKDRD
                     AAFEEFRLGYGELDAAQAKLSRKALEDVYLERFDRIYPTLDSTKQQLLLGFAYALGNL
                     TFRCLENERAGRCGWKKDEKATLREDHVRWARELLGFHLNTCLKDKCGAPPSAAKVRQ
                     ADMPCPLC"
     gene            4774..7707
                     /locus_tag="PLP50_01270"
     CDS             4774..7707
                     /locus_tag="PLP50_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA50204.1"
                     /translation="MTSVKPVLSRLLRAYAVWGALLVFAGLPSALYRLETVRNPPVVL
                     ELLPHADGSVEGTVAADGRRTRITHVSVKGGPWLAAGGVAAARTLWDLPAGTPVAYRE
                     ARAGEAPSVVSGTVSEHGATTAEAVAAATLALLAVVLAASGAAIAVAGVSSQALLSGA
                     ALAGLGHFAAARFLEAGASLVLDPATRGALVLGWVAFPRHFAFLSLAAFLSVFPSDLT
                     ARRLCRLLLAFVGSIALAQAVLVPLIQLEGPLDGLSVATQGRIVAWTRQLTALLFGTA
                     SLSSLVLIGMQVRAFRREPIDVATRRRATVVGAGLVAGLGPPLLLAFVQIAASRLVGR
                     PLLPPATMVLSLLPILLVPPALAYAMLSPRVVSVGLFARKALLLGFAQGTVRAASLAP
                     LAALGLLFYTRRDESIGHVFGDHLPFIFLALAVATAGLRYGGRTRDLLERAFFGTRKA
                     SAGTLADLAAATRRALDASELSTALVEGIQKALGLEQVVLFVREELSGTFTCPGRPLP
                     PLDGSSPIVELAGKRAGPFRPTADGDPDAPAALTEVDRQWLDSTRARLLVPLRDASGT
                     LIALLAVADKQSELPFDSEDEQFLAAAAAAGALTLENLQLRSSSSSRSAGGRPADAPL
                     TQAETDPEAARLCRRCARLFAPETGGTCPDDETFLEPAPVPVLLAGKYRLVRRLGAGG
                     MGVVYLARDVALARDVAVKTLPSLAAGSVRRLQREARAAALLVHPNLGLIFSSESWRG
                     TPMLVLEYLPGGTLSDRIQQGPVSPACAAAWGTALASALEAIHARNVLHRDVKPSNVG
                     FDAAGTPKLLDFGLVRLLDEVEAAPIPGLPPEVPESHGSSVSATRTSASHVVGTIPYL
                     SPEALQGRPPTPRFDLWGLAVTIYESVTGVNPFAATSNSRTVDKILTRSAPDPRTIRP
                     DCPGPLAELLLAALARTEAKRPASARELREGFEAAARGLSEAPPDLDRPAPAGDPHRA
                     PTGGR"
     gene            7990..9339
                     /locus_tag="PLP50_01275"
     CDS             7990..9339
                     /locus_tag="PLP50_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50205.1"
                     /translation="MTERSTPPPEGSATVGARGTVPPELTETGKLRILTAQEIEAARS
                     ETARTQEALKESEARFRTLFESSPNGIFITDPETLEILDCNAQACEMNGYAREELVGR
                     SINDLHPEEVRAQTKGGPEGRARFVEQLREKGVITVESVHVRKDGTTFPMETSMCLLE
                     LGGRGVVMGIDRDISERRRAEEELARHREHLEELVASRTAELAVERDRAEAADRLKSA
                     FLATMSHELRTPLNSIIGFTGVLLKGLGGPLTAEQSRQLGMVKSSAQHLLALINDVLD
                     LSKIEAGQLRMSFAPFVLKDAVEGCVRALAPAAEKKGLVLAAKVDPASETIVSDRRRV
                     EQVLLNLVSNAVKFTEAGSVTVSAAREGAWVVTRVRDTGIGIAAEDLPNLFRPFQQLE
                     SGLTRRFEGTGLGLSICRRLVEMLGGEIAVESAPGRGSTFSFRLPAGEAPAEIGPEG"
     gene            9339..9719
                     /locus_tag="PLP50_01280"
     CDS             9339..9719
                     /locus_tag="PLP50_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50206.1"
                     /translation="MSGNVLVVEDNEQNLYLVRFLLEGAGWTVVAAPDGPRALEEARS
                     GRFDLVLLDIQLPGMDGHAVARELRRIPALDGVPIVAVTSYAMLGDRERCLEAGCTGY
                     VEKPINPDTFLDEVASYVRPEVRP"
     gene            9716..11293
                     /locus_tag="PLP50_01285"
     CDS             9716..11293
                     /locus_tag="PLP50_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50207.1"
                     /translation="MTRVLVVDDESQNRYLLEVLLGGEGYEVVSASNGREALAAARRR
                     APELVISDILMPEMDGFLLCRAMKADATLRNVPFVFYTATYTEAEDERVALEAGGDLF
                     LRKPTEPDTLLAAIRRLLEERIPVSDEGPTRGLAEEVAFLARHDQALARKLEKKHHEL
                     QESEARYRSYFRSSPVAILVTDDTGRLLDANPAAGLLSGRDAVELLGANVSLLLGEGG
                     DDSPLRLAAGAPSAPSRTTAWRLRRKDGALRHVSLTAIRLEDGRVLAFCEDDTERVRA
                     EEAVRQVNADLERRVRERTAQLEHLNAELEAFTSSVSHDLRAPLRALDGYAAMLEEEA
                     ERLDEDGRRHLGAIRRNVRRMSELVESLLSLSRAGRQPLRTGPIDMERLARSVLDELL
                     VPGERERTEVVFRPLPAAVGDESLVRQVLANVVGNALKFSTRKERRRLEIGARNEGGR
                     TFWFVSDDGMGFEPAQAERIFRIFERLPGAEDIEGTGVGLAVVRRIVERHGGVVRAEG
                     TPGAGATISFSLGEGAG"
     gene            complement(11301..11786)
                     /locus_tag="PLP50_01290"
     CDS             complement(11301..11786)
                     /locus_tag="PLP50_01290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPA50208.1"
                     /translation="MGSLDGIEVRTIDGKAETLAKHAGKALLVVNVASKCGFTPQYAG
                     LEALWKRYRDRGLVVLGFPCDQFGHQEPGSEEEIQAFCSRTYGVTFPMFAKVEVNGDG
                     AHPLFRHLKKAAPGLLGTEAVKWNFTKFLVAPGGAPVTRFAPNDAPESLAAAIEKALP
                     S"
     gene            complement(11863..12105)
                     /locus_tag="PLP50_01295"
     CDS             complement(11863..12105)
                     /locus_tag="PLP50_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPA50209.1"
                     /translation="MSEPKIAAKEPVRVSLEAGKTYAWCRCGLSKSQPFCDGSHRGTE
                     LAPLVWKAEEAKEAWLCQCKHTGNAPFCDGTHKKLP"
     gene            complement(12179..12640)
                     /locus_tag="PLP50_01300"
     CDS             complement(12179..12640)
                     /locus_tag="PLP50_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50210.1"
                     /translation="MKGAKLDGYVVWKDARTKAAPGNVRVNNGAPFPSPSDSGELLTL
                     GGRAEVVPATGWELRAEGAAQRGRRDGRDLRAFGFTGRLARRFGRGRRHRAHLGFEHL
                     SGDDRASGRDEAFDAHVSAHPHAEYFRPGDFYAAHRRDESSFVRAELNLAF"
     gene            12809..13795
                     /locus_tag="PLP50_01305"
     CDS             12809..13795
                     /locus_tag="PLP50_01305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA50211.1"
                     /translation="MESRTLGTQGLVVSAQGLGCMGMSEFYAGRDDAESVATIHRALD
                     LGVTFLDTADMYGPHTNEVLVGTAIRERRSEVVLATKFGNMRGPDGAFLGVNGRPEYV
                     RACCEGSLARLGVEVIDLYYQHRVDKSVPIEETVGAMADLVKEGKVRFLGLSEASPAT
                     IRRAHAVHPVSALQTEYSLWTRDPEDGLLATCRELGIGFVAYSPLGRGFLTGRFRRFE
                     DLPEGDYRRLAPRFQGENFQKNLDVLAKVEEISREKGCTPAQLALAWLHAQGNDVVPI
                     PGTKSRARLEENVGALRVSLSPADLARIEAVAPKGFTAGPRYPEMAMRAIDA"
     gene            complement(13806..14753)
                     /locus_tag="PLP50_01310"
     CDS             complement(13806..14753)
                     /locus_tag="PLP50_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPA50212.1"
                     /translation="MQVVTGAFGYIGRYVARALLARGESVRTITTHVDKPNPFGPAVA
                     AFPFSFDDPAALTESLRGATTLYNTYWVRFDHGDSSFEKAVRNTSTLFRCAREAGVSR
                     IVQVSVTRCSLDSPLPYYRGKARQEEALRSSGVPFTIVRPTLVFGREDLLVNNIAWLL
                     RTFPVFPLFGSGSYRVQPVFAGDLAELAVEAALGEPGRTFDAVGPETFTFEELVRLLA
                     SAVGSRARIVRVPPALGIAAGRLVGLLLRDVVLTRDELRGLMDEKLTSEEAPNAPTRF
                     TEWLRAHRAEVGTAYTSELARHFRWRDGGKREREAARLG"
     gene            14847..15314
                     /locus_tag="PLP50_01315"
     CDS             14847..15314
                     /locus_tag="PLP50_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPA50213.1"
                     /translation="MIRRLIAARSFVLRASSSLAWLPLAATRVALGTVFVQSGWGKLH
                     DLPRVVGYFESLGIPAPQLQAPFVAGVELAGGALLLGGLFTRVASVPLAATMVVALLT
                     ARRADLATASDLFGAVELLYLLLLGSLAAFGAEALSLDALLARRFAPDEELKG"
     gene            complement(15311..16165)
                     /locus_tag="PLP50_01320"
     CDS             complement(15311..16165)
                     /locus_tag="PLP50_01320"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HPA50214.1"
                     /translation="MTSAGLPLATFQEWMQAVIVHPGGVDAGLRSLSARQNLPPSRLG
                     AVVRAHGALSPVERLGIYASMYPLRTVEALRSDYPALASLLGEERFGALVQEYVAAHP
                     STSFTLARLGDRLPSFVAAWGPKRGQGLRADVARLERAAAAVFDAADVALLDAPALAA
                     ALASEGESLRLLAAPAFAILAVRPSAVAVLDAVLDDAPVPTSAGRGLVYVAFHRRDFT
                     VLRRALDPVSGRLLAALVSGFPLGEALARAARRRPPARVVSGWLAEWSALGAFARAGV
                     APGPSAAS"
     gene            complement(16162..17028)
                     /locus_tag="PLP50_01325"
     CDS             complement(16162..17028)
                     /locus_tag="PLP50_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HPA50215.1"
                     /translation="MRNRFGLPMLGYGVGLRTVHFADHLERPGAADFVEAITENFLDT
                     GGRPLHVLERLAERTPVVLHGVSMNVGSVDPLDLGYLRAVKALARRVDARWVTDHLCW
                     TGVAGRNTHDLLPLPRNEETLAHVAARLRVVQDLLERPVHLENPSTYLELASSTMGEA
                     VFLGRLADLSGCGLLVDVNNVFVSAFNHGWDAEAWIDAIPADRVVQVHLAGHTDLGTH
                     LLDTHSDHVRDEVWALYARLVRRTGPVTTLVEWDEEIPALDVVLAEARKARAVAEAAL
                     GDRDGHSEREAA"
     gene            complement(17088..17474)
                     /locus_tag="PLP50_01330"
     CDS             complement(17088..17474)
                     /locus_tag="PLP50_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50216.1"
                     /translation="MKRNRPSNVYLSALVAGLVASSALAAGTLRADEKTPAPSGTPAA
                     TQKDKNACGGPNGCGGKKAGDKTGDKKAGPEATDRHACKGQNACKGKGGCKTEKNVCR
                     GHNACKGMGGCRTDGKASAQPTAKPA"
     gene            17671..18792
                     /locus_tag="PLP50_01335"
     CDS             17671..18792
                     /locus_tag="PLP50_01335"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPA50217.1"
                     /translation="MPETPTAPPLRSAPGRSGSAAALLAAGLALAAGFAACGPSRSLA
                     GPPAGKETNSEKVEKMKTVLSKSGYDVTPLSREEVARLAKKLSPESYRVTQKAGTEAP
                     FCGNLLDNKKEGTYLCVVCGLPLFSSEHKFNSGTGWPSFFREFDPSHVTRTIDSSHGM
                     ERVEINCARCGAHLGHVFEDGPAPTGERHCLNSASLTFLEKGAPVPPESRPVKAETAY
                     FAAGCFWGVQHWFSRGPGVLEATSGYMQGRTEKPTYRQVCEGDTGHAETVKVVYDANR
                     ITYPRLVEAFFAMHDPTQLNRQGPDVGDQYRSGIWTANDEQRKVADEVLAGLRESKAY
                     GERKIVTVVEPAKTFWPAEEYHQEYLAKNGAACHVTNPW"
     gene            18882..19439
                     /locus_tag="PLP50_01340"
     CDS             18882..19439
                     /locus_tag="PLP50_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA50218.1"
                     /translation="MSENEARERLAKLMVAYQGGDESAFESLYGELAGPVRAFLASLT
                     RNAARADDLLQETFFHVHRARQTYDPTRSAKAWVYAIAHNVFLMACRTDRRRRRNEEL
                     PAEDLPDLPVPAEAESLASRDLVSRALATLPVDRREALLLHHVEGLSFQEVGAVLGVT
                     TMAAKLRSHRAMGQLRELLAREGAR"
     gene            19436..20149
                     /locus_tag="PLP50_01345"
     CDS             19436..20149
                     /locus_tag="PLP50_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HPA50219.1"
                     /translation="MSPTPLPPALRAAVASDLRPVRPLASPARRALALLAWAAVAAAL
                     VLALRGLRSDAAELGLLLTWGVVLAEVAAGAGLVALALAEAVPARGPGRGAALLALGG
                     AAALFVALAAVTRRASRGMEVDDPLLSFGPSCAGLQGFIGLTAFAVAALLVLRAAPMR
                     ARLAGLLAGAGTGFLAEGVYHLDCPITHLSHVLVWHGSAILLLALLGFGFGLALEARE
                     RARMERRRAGTAARKGGEG"
     gene            20146..21195
                     /gene="selD"
                     /locus_tag="PLP50_01350"
     CDS             20146..21195
                     /gene="selD"
                     /locus_tag="PLP50_01350"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20197..20199,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPA50220.1"
                     /translation="MSDEVFRKLTSLSSCAGUASKLPPGQTAQVLRVLGDLFPAASRP
                     DVLVGLAEPDDAAVLDLGDGRALILTTDFFTPVVDEPYDFGAIAAANAMSDVWAMGGE
                     PVLALNLVAFPPQLSAATLAELLRGGADVVRSAGAVIDGGHSIQDREPKYGLCVVGFG
                     ETGRLLTKGGARPGDSLWLTKPLGTGTVTTALKRGLATEEEVAAAVASMKTLNRGAAR
                     AARAAGAKAATDVTGFGLLGHALEMAEASDVRFRFDWDALPFLPGAARLAAEMVFPGG
                     AFGNLEHFGARLARSRPLTDAEAMLLADPQTSGGLLVAVPEAGEGTFLSTLEREGGRT
                     WRIGDVGPGSGIEIR"
     gene            21394..22290
                     /locus_tag="PLP50_01355"
     CDS             21394..22290
                     /locus_tag="PLP50_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPA50221.1"
                     /translation="MRKLALGAMALLLATVPARAQEDYESCKDHPLFTRFPNSYIVDC
                     ASSQFDLRKFPVGQMKPGEEVLRSVEVEGPTWRINFQIKEGTTPPSGLQLMRNFETAA
                     KKAGGTVEGQWPGWCKANYDTEGMPDMGNSCTSFGLTMKFVKEAKETWVFFQANDADG
                     SYLMTVSEREAMKQEVAVNEIADQLKKDGFVSLYVTFDTGKATISPDSAKLLDDAAAA
                     LKAAAELKVEVGGHTDNVGSADANLKLSQERAQAVMAALVKRGIAAGRMTAKGYGQTV
                     PVADNRTEDGRAKNRRVELVKK"
     gene            22610..24181
                     /locus_tag="PLP50_01360"
     CDS             22610..24181
                     /locus_tag="PLP50_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HPA50222.1"
                     /translation="MKKILLALVGLAVAASLVVAQVRKKDAGPKPVEALRETYRTKTK
                     PSVDHALFAQLKGPFRRPQDVTAACISCHNGRHTEVMASSHWSWERVEYVAGKGIRAV
                     GKKNVLNNFCIGVAGSQQSCDKCHAGYGWADASFDFSNPMNVDCLACHDNGGTYAKKT
                     GGAGLPEPGLDLAAVARKVGRPQRENCGMCHFFGGGGNNVKHGDLDVALLDTTREVDV
                     HMGTDGADMACVDCHRAEKHQMLGKAYSLSSMNRNRVACESCHGAVPHEDDLLNKHGY
                     KVACQTCHIPEYAKVNATKLRWDWSTAGKLRDGKPYEEEDGDGNHAYLSIKGTFTWGK
                     DVVPEYAWFDGTASHYLLGETFDPSRPLVLNPLGGEYDVPEAKVAPVKVHRARQIYDT
                     KQRVLVQPKLFSAAPGDGGYWGDFDWNAAAAAGMKEVGLPYSGSYGFAETEMNWPVNH
                     MVAPKEKAVSCEECHAREGGRLAKVGGFYLPGRDRSRFLDGLGTTLLLATLGAVVVHG
                     GARIWFRRRRHAGGK"
     gene            24183..24842
                     /locus_tag="PLP50_01365"
     CDS             24183..24842
                     /locus_tag="PLP50_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPA50223.1"
                     /translation="MKREYVYPAFGRFWHWTQAFLILFLAVTGFEIHGSLRFFGYEQA
                     VRFHNGAAFSFLVLIAFAVFWHFTTDEWRQYVPTWKNVRAQAEYYLFGIFQDAPHPTR
                     KTKLKKLNPLQKIVYASLKLFVLPLVWVSGLLYMFYRYPQKHEVLALDVTTLEPIALA
                     HTAGAFALLVFLVAHLYLTTTGHTVTSNLKAMVTGWEDVEEEPAPGAGPSHAAATPDA
                     R"
     gene            24847..25383
                     /locus_tag="PLP50_01370"
     CDS             24847..25383
                     /locus_tag="PLP50_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPA50224.1"
                     /translation="MAVNDRPMNPYLAGVLLGLVLLLTVFVTGRGLGASGATKSVVVA
                     AVGAAAPEHAAANPFYAKLVGPGHENPLKAWLVFEVAGVLLGAFVSGLGAGRLKVGTE
                     RGPRVSVRGRLLFAAAGGALFGAGAQLARGCTSGAALSGMAVLSVAGFVTMLAIFGTG
                     FAFAWFTRGLWLPGKAKR"
     gene            25387..26580
                     /locus_tag="PLP50_01375"
     CDS             25387..26580
                     /locus_tag="PLP50_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA50225.1"
                     /translation="MGPFVPDLITDQLNLVVALAVGFLFGFVLEQAGFSSSRRLVGVF
                     YGYDLTVLRVFFTAAVTAAVGILLLAAGGLLDLKAIFVNPTWLWPAIAGGAIMGLGFL
                     LGGFCPGTSVCAAAIGKVDAMAFLGGSVLGVFAYAELFPRFAGFADSSALGPALVPDA
                     LGISAGLFVLLLAAAAIAAFAVGGIVERKVAGAAAPSSAFPRRLHVASAALLLAVAGL
                     AAALPDRKARLVAKVSAPGYAPHHLVRPMSPDELAFRIVDGDHRLRVVDLRGEAARAA
                     RPLPGAVSVPADDVLGREWRDVFARRMVQKVVVAPTEEEARTAAFLLVETGFENLAYL
                     AGGFPAFERTILTPGAPAGEPRFAPVVERFRTEARAALAERARLEKEAGTPAPRKAKK
                     VQGGC"
     gene            26676..28202
                     /locus_tag="PLP50_01380"
     CDS             26676..28202
                     /locus_tag="PLP50_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPA50226.1"
                     /translation="MDAVTLSRIQFAVSIGFHYIFPPLTIGLGVVLVWLEAWFLKTRD
                     PIYETAARFWTKVFALNFAIGVATGIVMEFQFGTNWATYSRFVGDVFGSALAAEGIFA
                     FFLESGFLAVLVFGWDRVGPKMHFFATLMVSLGSIFSSIWIVVANSWQQTPAGHHVVP
                     VLRDGVPLLRDGQPVMRAEITDFWAMVFNPSTVHRLVHVWIGAFLVGAFFVMSIAAWY
                     LLKGKHGEFARRSFDGGLVLATVSALAMLVSGHGQAGSVYRHQPAKLAAFEGHYVTGP
                     ADLNLFGFPDDATQSVKLGVAVPGGLGLLLHGDPAMPVVGLDRFRPEDRPPVGLSFQS
                     YHLMVALGMAFIGLTLLASFLRWRGTLYETRWLLWLFVPAVVLPIAANELGWVAAEVG
                     RQPWIVHPPVTWTADGTDVVVGPEGRVVYDERLGLRTLEAASPNVSAGQVLGSLLGFG
                     FIYLALGAVWVFVLDRKIKHGPDGASPPPGGSRDGALEAAALRAVHEGRMTDGGEARG
                     "
     gene            28213..29241
                     /gene="cydB"
                     /locus_tag="PLP50_01385"
     CDS             28213..29241
                     /gene="cydB"
                     /locus_tag="PLP50_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPA50227.1"
                     /translation="MSFEALALTWFVLEGVLLTGYAILDGFDLGVGILHPFVPRTDLE
                     RRISMNSIGPLWDGNEVWLVTFGGALFAAFPEAYATVFSGFYTAFMLLLVALISRAVS
                     MEFRSKMSSPAWRRFWDWAFFGGSFSATLLFGVAVGNAMIGVPIDPNGNVTGSVLGQL
                     GPYPLLVGAMTVALFAMHGGLFLYLKTEGDFQQRLREWMWRSWGLFMVLFMLTTVATV
                     AFIPRATENFRSFPWALVVVVGTILGVANIPRCLYRDRPGLAFLSSAGVIAGLVALFG
                     LALYPNLVTAAGDPSRSVTIFNAASSPKTLGIMLVIAAVGMPFVLTYSGVIYWTFRGK
                     VKLGEHSY"
     gene            29320..30030
                     /gene="prxU"
                     /locus_tag="PLP50_01390"
     CDS             29320..30030
                     /gene="prxU"
                     /locus_tag="PLP50_01390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959372.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:29563..29565,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPA50228.1"
                     /translation="MSEPVGCGRVSGAVSGEGPPPAPGPDTERKKEVLVTARVGQKAP
                     EFAAPSYYRGGFTPVKLADFAGKWVLLCFYPGDFTFVUATEVSAVADNYARLQALGVE
                     VLSVSVDSHFVHKMWNDHELVKMVDGGVPFHMASDQAGNIGRAYGVWDESQGIELRGR
                     FLIDPDGIVQAMEVMTPPVGRKLAETIRQVQAFQVVRASKGTEATPAGWMPGDPVLRP
                     GPDLVGNVWKVWKPSMEK"
     gene            30062..31453
                     /locus_tag="PLP50_01395"
     CDS             30062..31453
                     /locus_tag="PLP50_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA50229.1"
                     /translation="MTKRAASDLPVSPASSEAILESISDGVFTVDPEWRITSFNRAAE
                     RITGVPRREAIGRRCAEVFRSSTCGPSCALQKTLRTGKPVIGKSTYIVVADGTRVPVS
                     LSTAVLRDAKGRVTGGAEIFRDLSEVEALREELEGRSRVGDLSTRSPLMQRVLEALPA
                     IAASPSTVLLLGETGTGKELVARTIHALSPRAKGPFVAVNCGALPDTLLESELFGHTA
                     GAFTGAVKERAGRFALAQGGTLFLDEIGEVSPALQVRLLRVLQERTYEPLGSSRSART
                     DARVIVATNRDLAARVREGHFREDLYYRVNVVRIELPPLRSRKEDVPLLVEELVARFN
                     RLQGRAVKGITAEALSLLMAHDWPGNVRELANIVERAFVVCGERRIDIGDLPDELAGR
                     LRPGAPELDVRSAHAVLDAEAIRSALERHGFNRLEAAKELGIHKTTLFRRIRKLGMEL
                     PEQDGRSARSETE"
     gene            32147..32497
                     /locus_tag="PLP50_01400"
     CDS             32147..32497
                     /locus_tag="PLP50_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPA50230.1"
                     /translation="MPRRDGTGPMGQGSMTGRGAGPCSGDVAQGAPGQGRGRGFGLGF
                     GRGFGRGGGAGFGRGWQWWRRGAVAGAAVPPAAVSDAAREEEALRMRIDALKEELACL
                     EKGVRAMKGDADKG"
     gene            32501..32944
                     /locus_tag="PLP50_01405"
     CDS             32501..32944
                     /locus_tag="PLP50_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPA50231.1"
                     /translation="MAKIGIIICARYKDCGGGKCLRSMRERRGAFAAYPADEPLEIVG
                     YGSCGGCPGGNVEYVPAEMKKNGAEAIHLATGLVVGYPPCPYIREFKQYIETAYGLPV
                     VVGTHPVPEKYRKVHEQLPFWDDSGMAELAGPLLNEDPVIQKAYD"
     gene            32959..33321
                     /locus_tag="PLP50_01410"
     CDS             32959..33321
                     /locus_tag="PLP50_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPA50232.1"
                     /translation="MKIAFSTSGESLEAPLDSRFGRAPYFLIYDLDSRQARVVDNGAS
                     RASSQGAGIQAADSVVRAGAGAVVTAHCGPKAFQVLHAAGIKVFSCHAATVGEALDMY
                     RSGALVEQTGADVAGHHA"
     gene            33318..34163
                     /locus_tag="PLP50_01415"
     CDS             33318..34163
                     /locus_tag="PLP50_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50233.1"
                     /translation="MKVAIASGKGGTGKTTVAVNLARSWGGPVQLLDCDVEEPNAHLF
                     LGGEKGEEVPVFVKVPSVDEALCDGCGECGRFCEFHAIVVVGRSPIVFPGMCHACGGC
                     AKVCPRGAIREVDERIGVVETRRSGSVSLTLGRMDVGMSMAPPLVHAVRARIQGGSPA
                     ILDAPAGTSCSVVATLRETDFVVLVTEPTPFGLHDLTLAVDLVRELRLPFGVVVNRVG
                     AGDDRVHAWCRKEGVEILLEIPDDRRIAEGYARGRLLVEALPEYGDLFRSLAGTIAAR
                     VGVAA"
     gene            34160..35152
                     /locus_tag="PLP50_01420"
     CDS             34160..35152
                     /locus_tag="PLP50_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50234.1"
                     /translation="MSAAERAFRHVYGPVPSRRLGRSLGVDLAPFKTCAYDCVYCQLG
                     RTTVKTAERKEYVPVEEVLSELREWLRRGEACDYISLAGSGEPTLHSGLGTIVRAIKG
                     MTKVPVAVLTNGSLLFMGEVQDALMEADLVIPSLDAGDAHLFQWVNRPVEEISFDRMV
                     EGLESFTKRFRGEVWLEVLLLEGVTAYPTQAEKIAALVRRIGPARVQLTTASRPPAEL
                     FARRVSEGRLLALASLFPGRVDVIATREEPDASVGSSPGTGAPAILTLLERRPCTATD
                     VAVGLGIHPNEALKVLDRLVVAGTVKTVASADRTFYIVAERGVPAGSPEGGRQR"
     gene            35149..36021
                     /locus_tag="PLP50_01425"
     CDS             35149..36021
                     /locus_tag="PLP50_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50235.1"
                     /translation="MREIVVVSGKGGTGKTSLTASFAVLADRPVVADCDVEAADLHLV
                     LSPTVVERHEFRGGREAVIREEDCLGCGICLEECRFDAVRRIEKGGGRATYAVDPTAC
                     EGCGVCVRFCPEHAIDFPERRSGEWMVSATRCGPMVHARLATAAGNSGKLVSEVRQEA
                     RRIADAEGRSLVLVDGPPGVGCPVIASVTGASLVLAVTEPTVSGEHDLERVLSLARHF
                     GVRAAVCVNKWDLDPGMADRIEQLASKAGARLAGRVRYDRGVTAAQMLQRAVVEIDTP
                     SAADVRRVWESLAG"
     gene            complement(36170..37564)
                     /locus_tag="PLP50_01430"
     CDS             complement(36170..37564)
                     /locus_tag="PLP50_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA50236.1"
                     /translation="MDRETQRAPDWSNLEAVFESLGRALVVLDEGFVIIRASRSLERM
                     AGEGVAAEVIGQPVESLFGPRLFGPEETLRESLRLGRREEGRRGVLRCGRDSARLVSI
                     TTTPLAPGVVDACDPRARYLAVIRPAEDDDTILQSAAVSHGLVARSPQMLRIVHLVES
                     LHSSEANVLITGESGTGKEVLARALHAHSPRSSGPFVAVSCAALPADLLESELFGHVR
                     GAFTGAVRDRTGRLDLARGGTLFLDEAGEIPLPVQVKLLRVLQERVFERVGESTPRPL
                     DARIVAATNRDPKEAVRTGRLREDLYYRLRVVPIHIPPLRERTEDVELIARYLLARIG
                     GRAGRAVQLSPDTLAALERYEWPGNVRELENALEYAVALGRGQTVQLEDLPPEIRAPE
                     PFASGGDRSRERSLATPRQARDPASERDDPEEGPIRAALERNLWNRSKAAADLGMSRT
                     KLWRRMRELRIGVR"
     gene            complement(37609..38007)
                     /locus_tag="PLP50_01435"
     CDS             complement(37609..38007)
                     /locus_tag="PLP50_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPA50237.1"
                     /translation="MNLCIPVTADRGLESPVSGHFGSAPLYLLVDSETRATRTLSNAR
                     AVHEHGACRPLDALAGERIDAFLVGGIGAGAIMKLQSAGIRVFRATAPTASVCLDAFL
                     RNELEEMDPAGACAGHGHDHGHGAGLPSRG"
     gene            complement(38108..39580)
                     /locus_tag="PLP50_01440"
     CDS             complement(38108..39580)
                     /locus_tag="PLP50_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPA50238.1"
                     /translation="MPDALPDVLHELEADAVPSAAETLVSIAAEYLAETRSGDGPVST
                     PLTPRELARLFDEPLPRRGRPLAEVAERLRREVVPASNRLCHPRSMGHQVSAPLPAAI
                     WTEPLAAALNQSLAVFEMSPAGTAIEGRVVRWMCDLAGFGPAAGGTFTSGGTEATFTA
                     LLAARAAALPDAWTKGVGAEPPVVLHGEHAHYAVSRAVGALGLGTDAAVAVPSRAFRM
                     DVAALEAELDRQAARGRRVMAVVATAGSTATGSFDDLVAIGRLCEARGVWLHVDGAHG
                     ASALLSSAHRGRVDGIARARSLAWDPHKMMLMPLSAGVLLVRDERDLDAAFAQRAPYL
                     FHGAEGERSPDQGKRSFQCSRRLDALKVWVAFQRYGAEGLGALYDGLCTTARALHAAL
                     AARRDFETLHEPESNILCFRYVGEEKLDAGRLDGINLRLREAFNRSGEGWITTTLLVG
                     RRVLRATQMNPRTTAADLERVVAGLARTGREVAARSADRP"
     gene            39672..40844
                     /locus_tag="PLP50_01445"
     CDS             39672..40844
                     /locus_tag="PLP50_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPA50239.1"
                     /translation="MPRRSASLRLAGLAILVTVAALARTAPAHEVRVRRPLRVPDIPG
                     YVTLRCDFHTHTVFSDGSVWPDIRSEEAWREGYDAIAITDHIEYQPHKDDLPTNHDRP
                     FEIAAPLGEKLGVLVLRGSEVTRKMPPGHFNAIFLKMSKPLETPEWRDALKAARAQGA
                     FLFWNHPGWRGHQKDGVARWYPEHDEILAAGLMDGIEVANDREYYPEAHRWALEKDLA
                     ILANSDIHDPSNLAWHVHEGDRRPVTLVFAKERSEEGIREALFARRTAAWADGKLAGR
                     ERELRPLVAAAVTIENPKLAVRAGDEGYVRLRNDSDLRLVLEGEGAAGEVVVPGTLVV
                     APNGTSLLPVRVPKKTAPGPRRLELVYAVSNVRPTPDAGLPFPVAIEVEVLPPAPK"
     gene            complement(40854..42446)
                     /locus_tag="PLP50_01450"
     CDS             complement(40854..42446)
                     /locus_tag="PLP50_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HPA50240.1"
                     /translation="MPSDKVLLLTAMGAYLALTIGVGLWYSRRSARSAEAYFLGERGL
                     GPWVAAMSAEASDMSGWLLMGLPGVAYFSGAGEAAWTAIGLALGTFACWSLVARRLRA
                     HSIVAGNAFTLPAFFSARFRDGRRLLSTIAAGMLLFFFSIYVGAQFVTFGKLFGYVFG
                     MEGLYARMVVFGALFVLAYTFVGGFLAETMSDFIQGTLMFCALVLVLSVGTAKAGGVG
                     ALLAELGAIPRFTDLFGVATPLDAAGAAVTAAAGNAQALVDGNPAFGPGVGYGFLAIL
                     SCLAWGLGYFGMPQVLLRFMAIEKPAMVSRARVIAVTWCVVSLFSAVAIGLIGRALMP
                     DRLLTASAAETVFIHLAVDFFPPLLAGLVLSGIVAASISSADSYMLIASSSLANDLVQ
                     NFRAEPLPDRSVLWLARGTQVAVTAFGILVALSGNDSIFRVVSYAWAGLGAAFGPLVL
                     FSLFWRRTTLAGAVAGMLTGGIAVVAWKNLVAPLGGAFAVYELLPAFLLSSLAIAVVS
                     RLTPAPPAEVTAAFDEARTFPG"
     gene            complement(42573..43448)
                     /locus_tag="PLP50_01455"
     CDS             complement(42573..43448)
                     /locus_tag="PLP50_01455"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPA50241.1"
                     /translation="MKKTALLAALLLATPLLAQPGPRGGQGNGPGAGVPAAPAAPAAS
                     PFATYFASLPKEPLGSAEAAEIAFLREEEKLARDVYRALFAAHGDRAFANIAAAEQRH
                     MDLVKLLLDRYELADPAAATAPGEFKDSRLAALYTDLVAKGKVSLVEALKVGATIEDL
                     DLADVAKALAAADNRDVDAVMQNLAKGSRNHLRAFAGRLSALGAPYAAQYLPADEVAR
                     ILAAPRERGAVDETGKLLPGTGTGPGRMGRGRGRSMGAGNGTGTCDGTGPGAGTGPGA
                     GFGPGDGTGAGCPNR"
     gene            complement(43675..44949)
                     /locus_tag="PLP50_01460"
     CDS             complement(43675..44949)
                     /locus_tag="PLP50_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA50242.1"
                     /translation="MARLLVVDDEPSFRALLRDILEGAGHGVTEARDGAEALAFLERT
                     TYDLVLTDRRMPAVDGLELLRRVRARTSPPPVVVLTAHGSIPEAVEAVRLGAADYIAK
                     PLPSPEALVDLVASLLPREDDGEIVTADPAMRELLDLVDRVAPRDVAVLVTGESGVGK
                     ELVARRLHARSPRAKGPFVAVNAAALPETLAESELFGAEKGAFTGADQARAGRFEEAS
                     GGTLFLDEVGELPLGLQSKLLRVLEERVVRRLGGSRDLPVDVRLVAATNRELSRETEE
                     GGFRQDLYFRLAVVVVNVPPLRDRPGDVPLLARHFAEKLAARHGVPVPSLTDDALAAL
                     SAHAWPGNVRELRNVLERAVVVRGGEAIRGADLGLAPASPAFAPLDRAHREREALLAA
                     LRRTGGKREEAAKLLGISVRTLYYRLKQWGIG"
     gene            complement(44951..46186)
                     /locus_tag="PLP50_01465"
     CDS             complement(44951..46186)
                     /locus_tag="PLP50_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA50243.1"
                     /translation="MIRSNRTAVWIVVLSVVFASALLGTAGLASRTLRQRDAAVRDGV
                     LSRAAHELEASLRGATPEEATAVLEEFLSRNAKSVDGAAVIGPGGVLASAGADSVDGY
                     EVTAALGREWRPALLGAEPGSGGGFMGGRGPGAGAGRGMPPIRLRLRPAPALGREPAL
                     AGLVLAGGGAAALALVGLALVAARGLAERERREAAEAEGQRLATVARAGAGLAHRLRN
                     PLAVVKGTAQLLDGRVPEGERERVVRIVTASERMETILSRLLDFARPPDPQPATFDLA
                     ALAREVASRGVEGTAAGVAVRADRAVPARADREHVETILEELLANARAFDPEGTLEVT
                     VRAEGGRALVEVADRGPGLDLDPASAFEPYVTSRPEGTGLGLAIVRSLAVANGGTASL
                     APRPGGGCVATLALPGEGA"
     gene            46284..46490
                     /locus_tag="PLP50_01470"
     CDS             46284..46490
                     /locus_tag="PLP50_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPA50244.1"
                     /translation="MPGIYKKVELVGTSPVSFAEAVKAAVEEASKTVRHMDWFEVVEQ
                     RGRVHDGKVAEFQVTIRVGFKLER"
     gene            complement(46528..48651)
                     /locus_tag="PLP50_01475"
     CDS             complement(46528..48651)
                     /locus_tag="PLP50_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPA50245.1"
                     /translation="MRSLSLKKRPDGVAVLLFDTPGRPVNVLSSGLLDEAGPLMREIR
                     EDDAVVACVLASAKPDGFIAGADLDEVLAMGAAEAESISRGAHAWLDRVEESRKPFVA
                     AIHGAALGGGLEVALACRYRLATDDPRTVLALPEVMLGLLPAAGGTQRLPRRIGLQRA
                     LPMLLTGKRLRAAQAYRCGLVDALTSPGGLLETAAKAALALAKGTLRPPTDRRPIAET
                     PLALPPLRAWAIGKARAEVLRKTRGLYAAPLDILACVEKGLADGLAAGLEEEAVRFGR
                     LAASEGAKNLVRLFDAMTALKKAPKGSPAPRPVNRLGVLGAGLMGEGIAAVSLPLAPV
                     VLKDVSEEGLARAARSLRKGLDRRLRSAALTRLERDRRWLALKPTTKPEELAGCDLVI
                     EAVFEDLALKRKVLAEAEAVLAPDAVFASNTSALPIGEIARGAKRPERVVGMHYFSPV
                     PKMPLLEVVVAERSAPWAVATARAFGVAQGKTVIVVKDGPGFYTTRILGPFVNEAVVL
                     LDEGAAIPDLDAALLDFGFPVGPVALLDEVGIDVAAHVSRDLGKAFAARGAAPSATFG
                     KLFDAGYRGRKNGKGFYRYGPSTRPGGRKEVDRDVYAHFGGTERKPMRAADLVDRLVL
                     LMVNEAAWCLQEGVVASPRDADAGAVMGLGFPPFRGGPFRWVDAVGAAEVVARMERLR
                     TLCGKRFTPAPLLLEAARTGRPFHG"
     gene            complement(48648..49943)
                     /locus_tag="PLP50_01480"
     CDS             complement(48648..49943)
                     /locus_tag="PLP50_01480"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPA50246.1"
                     /translation="MAAKNVHGRTVVFAGGCRTPFLRAGAAFADVMAYELGAMALRGL
                     LDRTGLDPAAVDRVVMGTVLADPRTTNLARESALAAGLPASCPAFTVTAACASSNVAV
                     SGAVEAIASGAADVVIAGGAETLSDAPIRFGREVRKRLIRSQKAKGVLDWVKLLQGLS
                     LKDLAPDTPAIAEFSTGQTMGQSAERLAKRLSLTREAQDRWALRSHRKAAAATRAGLY
                     VEQVVPARVPPSFGPIDADDGIRGDTTLAKLGAIPPAFDRAFGTVTAGNSSFLTDGAA
                     ACLLASAEAAERLGLAPRARVAATALVAMEPLEELLLGPVAAVAKALDRAGVPAGEVG
                     VWEVHEAFAAPVLAFLKLLADRSYCRERLGRKDAVGRIDPKRVNAWGGSLALGHPFGA
                     TGARLLTTCVDRMEREGAEIGVVTTCAAGALGHAMVLAR"
     gene            complement(50070..54206)
                     /locus_tag="PLP50_01485"
     CDS             complement(50070..54206)
                     /locus_tag="PLP50_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPA50247.1"
                     /translation="MTGRFARILLVCLPLATVPVRGEEPWLEAPFEAEPAAVLRASEE
                     KLPGETEENSAWYRLLLDESRWTFEADGRFSMARRRVLRVLSHEGAEEMGSVAASWKP
                     WIEARPILRARVIRKDGTVLVLDEKTIAESPASSDPLLFDDRKVLRVPLPGMEPGAVV
                     EWEIVKTRTSAPPTGTLGSQLLAYSYPAGRIRCRVEAPSTLPFVWKAIGIDLAPKASL
                     ASGRVVRVWERRNVPPLEDVESDLPPEVPANPLVEFSTGTSWNQVATSYRAVVEERIG
                     EGPLDAASLPAGPFPSRLHEAAALAEWVRREVRYTGLLFGDGAIVPSSPAEVLRRKFG
                     DCKDQATLLVAALRLRGFDAAVALLRVGEDPDVTPALPTLDAFDHAIVQVAGLRPEGV
                     FVDTTSRFERLGEVPARLQGRLVLVCRPETQGPSRIPIAPSASNTSRTEIVVRIPEAG
                     EATASEKTVYSGVFEANRRQAYDGTGESDLKSSFERWAESRFGGGKVLRGVVSPSRDL
                     STTFTLETEVARPAFVRADDERISLLIAADGFLSELPDPEDGERKNELPALPFRQEYR
                     YSFVPPPGFSPVLLPEEETRSLGPATYHRKAVAREDGSVTFEVEVELTRARLKPAEVV
                     ALREGLAELREAEPMAFTFQHGSSVAAARGDVARGLEILREEIRRDPAKAAPHRRLAR
                     LYLASGLGDAARREAREAVKKEPASARSWYLLGLVLEQDRWGRLRQKGWDPVEAEAAL
                     RKSLELDPDDIEVRFELAILLEHDREGYRYADSARLALALDEYAKLVEKSPDATVVAR
                     NYALTLLRADRPADAAAFLEKRPDVDQMIGLRIAAVAASRGTARALELLRQAVPEPAA
                     RRKAAGEIGSLLLVLRRYPEGAALMDEGARGGDGAGVAAVHAGTARRLKRHDGPVPAP
                     VRGNALSAVHALTAELFPRDGRIESVEKLYVKAVRAARDAHGTSLLRKFLEDEVATIR
                     RSALSSGAALAFLRDTALATELSAEGSAADGWLVSGTVPSVGQAPRQIRVWVAEEDGV
                     PRVVSDDGGSRFLCWYALDLVTRGRVADARRWLERAALEVEVHEEESDLVLDPLLPRL
                     RPAEGASAAELRLAALAGLAGTPLPDPLLDELRRAQREKPEDPLRAALLHRSLVARTG
                     PDSKRTKKETQVLLEEAIGLLPVIRADRASTESVAMFDIGLKARVGRVEEALAEAEEH
                     SRSHPSCRLVGSSLVSLRTTSGDVTGALEAADKLMQRPAPSANDANTSAWARYVAGKV
                     DEKALSLARQAVEGSRRSNPAILNTLAAICAELGRHEEAHSLVLRSMELQGLDEPMPG
                     DWLVVGRIRESFGLVDEAIEAYGRASKDEEEQASFPDSVGEIAARRARALRTSKVGAD
                     "
     gene            complement(54370..57276)
                     /locus_tag="PLP50_01490"
     CDS             complement(54370..57276)
                     /locus_tag="PLP50_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA50248.1"
                     /translation="MKPLLAAVFAVLATASPADATPPFPAEWLRPRTDVRGPLEKADP
                     RLLAPAPSGEGAVELLVTLRLPEEARRVGPDAQRKRERRAFQTRLAEELELDYAPFGV
                     RVERTFAFVPALLLRAPRAAAAALAGDPRIERVRPNGRVRALDTEGDGLMRVPELRAA
                     GVDGAGVTIAVLDSGIDYTHPELPLGTKVVNLYDAIDHDGDARDGYGHGTPVAGIIAG
                     LTTGVARGAKVAAVRVLDTRGEGSDAQVLEGIDAVLASVAEGNPHAIRVVNLSLGGYF
                     DGEAPPEPGRCDLLAPDYKAAFDLLGQAGILVVAASGNGGCTGGVVLPACVSNALVVG
                     AVYDAAVSPQAFGRGHCLPSGCSDSAPAADAIACYSDTGDRLDVLAPAHCATAPRNGG
                     GTHTCFGGTSAAAPYAAGVAALLFGAEPDRRPTEVKAALRETGKAILDARNGVTRNRL
                     DAPAALAALRSIPAGTARAELLVPVVLDVRGVGEARFLSELTLTNRGTTAARVEATYM
                     PATGMFPGAAGGGTVADTLAPGEQRVIADAIAWLRTLKGLPIPEGPGQAGSLRFVFTG
                     LSPDADVVSARVRTTAAACGGAAGLSYAAWRMGDGSTAPLRLFGLRSGPAERSNLAVV
                     NMGTEGTVTPRIVLRSGDGASVYEVPAALLPPLAPGEWRQVNDADLLGAAGFAEATAL
                     VSRAAGTAPLGTYAVFNDNLSHDGSFVPAVPDDAFGGELVLPVVVETPVFESELVLFN
                     PGAEAVSVSLVYTESLATPKGRVGAVSIDLGPFQQRILPRALDTLRGLGLPLGPRGAA
                     SYAGTLLVLFQSPFTTLVPGLAGAKTAAAGSGACAGKGSFGLFYTAVPATSAAAVEAV
                     VDGLRQTPTTRSNLALFNPSTTVPVSVAYDVHDGATGAKVLSSVPFDLPPLGWSQVDR
                     VLSGTAVTEGFVRVRRASAGGTFAAYGVLNDGAAPGERTGDGSYVAGEAYDPPN"
     gene            complement(57344..59011)
                     /locus_tag="PLP50_01495"
     CDS             complement(57344..59011)
                     /locus_tag="PLP50_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50249.1"
                     /translation="MPTHRLLRRGDAAFAVALFVSLTALSWLRWASFQGDLNREWTVP
                     MRLAAGERLWSEVGYYYGPLAPHAVAAAFRLFGATVATYVGFALLSAAATLAALLAAG
                     RRMLSPAANAGAAAVAVGVLAFAPENGAFAAPYAMAALLAVGLVWGAFLAAASGRAAL
                     AGLLGALALLAKPETLPALLGVALAAGTWRSAARLVGTAGVLSAAGYGFAMAGIPVGD
                     LVSYGPLRHFVMPPEFRELYLRVSGLHPSLLPRGVGGLLAGAALLGGWALCAAALAKR
                     EGARAAAGGALALAGLLVGAVVLREPVVTTGVRGLPLLAALASVAAAVDLRRGEREAR
                     LPLAASLVGLAFAWRTALWTVPVYPYAPLGAVSTLPAVAWLLACRIPAAAPPEARRRG
                     ALLAVVPLLLAPVLFLPRLVEFYRAPRTEVAGPAGRWFPPGDEGALFSRVAAHLRSVG
                     VKDRDLVVLPEAGALSFLLGVRSPLRFEHVLPGCVDDRVDRDLADGLDRARPARVVVV
                     SRPTSEFGRSAFGRDYGTRIAARLARDYAVEATFAEGTAAAVVLRRR"
     gene            59182..62778
                     /gene="nifJ"
                     /locus_tag="PLP50_01500"
     CDS             59182..62778
                     /gene="nifJ"
                     /locus_tag="PLP50_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPA50250.1"
                     /translation="MTTRPMVTLDGNEAVASVAHRVSEVIAIYPITPSSTMGEFSDEW
                     SALGRKNVWGTVPLVSELQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKIA
                     GELTPFVMHVSARCVATHALSIFGDHSDVMACRQTGFAMLASSNPQEAHDLAAIAHRA
                     TLMAKVPFLHFFDGFRTSSEVAKIELLGNDDLLAMVSEDLVSAQRKRALTPEAPVLRG
                     SAQNPDCFFQAREACNPYYDACAGKVQEAMDAFAKLTGRAYRLFDYFGHPEAERVIVL
                     MGSGADVTHETVEYLLARGEKVGVVKVRLYRPFDASAFLGAVPKTAKAIAVLDRTKEP
                     GSVGEPLFQDVVTVMRNAREDGTSPFAVEPKVVGGRYGLASKDFTPAMVKGVFDELKG
                     AKPKRGFTVGIVDDVTHLSLKWDPDFDTEGDDVTRGVFFGLGADGTVGANKNSIKIIG
                     EETDNYAQGYFVYDSKKSGGITISHLRFGPKPIRSSYLVKTAGFVACHQFGFLEKYDV
                     LEYAKPGAVFLLNSPYGPDEVWDKLPVEVQSEILEKKIRFYVIDGYEVGAKTGMGRRI
                     NTVMQTCFFAISGVLPREEAIAQIKKSIEKTYGKKGAELVRMNFEAVDQTLENLHEVK
                     VPAAATSKLKRPPIVPEEAPDFVQKITAVMLSNKGDLLPVSAFPVDGTFATATARWEK
                     RNIAIEIPVWDEKLCIQCNKCAMVCPHAAIRAKVFDPAVLEKAPGTYKSVDYRAPEFK
                     GMKYTIQVAPEDCTGCTLCAAVCPAKDKANPKHKALDMAPQAPLRQAERENFKFFLGL
                     PEPDRTKLKVDVKGSQFMQPLFEFSGACLGCGETPYIKLMTQLYGDRLLIANATGCSS
                     IYGGNLPTTPYTVNSEGRGPAWANSLFEDNAEFGFGMRLALDKHAEHAAELVKELSGL
                     VGDELVSSLLTADQSTEQGIAHQRERVAALKAKLAGQKGLPARRLTDIADYLVKKSLW
                     IVGGDGWAYDIGYGGLDHVIAMGRNVNILVLDTEVYSNTGGQASKSTPIGAVAKFAMA
                     GKSAGKKDLAMMAIAYGNVYVAKIAFGAKDAQTVKAFQEAESYDGPSLLIAYSHCIAH
                     GYDLSLGLEQQKLASESGYWPLFRWDPRRVAEGKPGLVLDSSAPKTDVGRFMANETRF
                     RMLEKTNPERAKMLLDLERHEVASRWAIYENLARGADPVTAVAAAQTTPKAN"
     gene            62797..63789
                     /locus_tag="PLP50_01505"
     CDS             62797..63789
                     /locus_tag="PLP50_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPA50251.1"
                     /translation="MDLSTTYLGLKLPHPIMPGASPMVDKIDLVKRMEDAGAAAIVMH
                     SLFEEQLMREELATYHHIDVHADSFAEAMTYLPRPDEFNLGPDQYLEQLVRIKQSVDL
                     PVIASLNGFTSGGWIRYAKLMQDAGANALELNVYYLATNADEGGDEVEQRTIDILKAV
                     KQAVTIPVAIKLSPFFSSMASMARRLDEAGADGLVLFNRFYQPDIDVESLEVEPTLKL
                     SDSSELNLRLRWLAVVSGRVRASLAVSGGVHTHLDAVKAVMTGAHAVQVVSAILQQGP
                     DVLRRIREGLTQWLEEHEYDSLEQMRGSMNLLRCPDASMFERANYIRILQGYKV"
     gene            complement(64019..64450)
                     /locus_tag="PLP50_01510"
     CDS             complement(64019..64450)
                     /locus_tag="PLP50_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50252.1"
                     /translation="MPEEVWILLVEDDDDDAELALRALARCELAPRVVRARDGEEALD
                     VLLGRGEAAAGTVPPPALVLLDLKLPKVDGIEVLTRFRADGRGRDVPVVVLTSSMLPA
                     DVRACYQAGANSFVVKQVEFAEHARTLREMAAYWLQVNVRP"
     gene            complement(64443..66245)
                     /locus_tag="PLP50_01515"
     CDS             complement(64443..66245)
                     /locus_tag="PLP50_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50253.1"
                     /translation="MTLDVRTLLALFALTQALQVVVLSVQYALFRSYRGTGAFLLWSA
                     AVGAGSVAFLFRDHETLGRVAIFAQNGLIVFGTLCLWAGLCRFHGRDEDRRLFGAVLV
                     PYLAAHAFLLWVVPDIDLRAVALSAALVVSALLSARELWRERTGESRPVALVLVAGLL
                     AHAAFFAYRGAVLLIGAHVGDVWGSAAFNVAPYVDGFLLTNLVTFGIVILVNRRVNAE
                     VREARDHFRRLLDTIPDAVLMTRVSDGICRDANEGFEALTGYRRDEIVGRSSTELGVW
                     VDPRARDRVFERALESDATRDLELEGRRRDGTPFVGSVSARRVDVRGEPHVLSVVHDV
                     TGRYAAREALQRSDREIRELNAGLERRVAERTAELTAKNAELEAFVHSIAHDLRAPLR
                     AIEGFSGILEEEHSARLDDEGRRLLARVRGGARRMDRLLRDLVEYARAGSAGLRHERV
                     DLGVLARGAFEDVVPAEAREAWRFVVGDLPTARGDAALLRVVLRHLLANAVKYAAAGP
                     GRSVEVRGRVEAGHVVCEVVDDGVGFDPAHAGRLFRVFERLHAGEGDDGTGIGLAIVR
                     RIVERHGGSVHAEGRPGQGATFAFSLPETGESDA"
     gene            complement(66311..67633)
                     /locus_tag="PLP50_01520"
     CDS             complement(66311..67633)
                     /locus_tag="PLP50_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50254.1"
                     /translation="MASRTAFPRVFGELVHDSFSITSFFTGLLAALPTGIVLLFALSG
                     PRTKGDAVAVLVVWMGTVLVFVSRFAQVGVAGDTEGGLFSSAAGPWSGTMAAAARLLL
                     LWAAWAVPFVVWGLLKGAGTPDPAIGLVPIGFPRLPFLISLWSGLGVVLSFAFVAVAA
                     VAPSFGALFSPALWRTLFAGRIGELFLAIAGVFGPPLTVLLVVLPVYGALMPRQPKLA
                     LTAFVPLLLYAVGMVLTLQGKLCGAFAAASLAEEADEPLDVEVAAHAGTASVAPAGAA
                     PAEASTADPESLQAIWQARLTAGDVEGALEAARMAIPAGLAKGSPKAAAAIYRQHLDR
                     LDALGLDRPALSLLADQLLRDGDVAAAAWTFSQTLDAEPGDAKAFKGLLRVAEHHLEK
                     AKAPLEAVRVYRYLLERAPGSPLAEHARDLLADAERKAAREPAPPARS"
     gene            67775..68611
                     /locus_tag="PLP50_01525"
     CDS             67775..68611
                     /locus_tag="PLP50_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPA50255.1"
                     /translation="MKLRPTLLLAAVALAAPAFAQFETPQPSPKATLSQRVGLTDVTI
                     SYSRPSVRGRAIWGDLVPWDKPWRTGANAATTLTFSDDVTIEGQKLAAGTYSLVTIPG
                     KEEWTVVFNTDTKLWWETQYDAAKDALRVKVKPEAAPMTETLHIGFPSISATGAVLAI
                     EWEKVRVPVKLGVEVDGKLKKALGSAEASAWQSPLMAARYYWEQRQDRAEAWKQLEKS
                     IAASRNWANVSRKARFLAEEGKTAEAVKAGEEALALAKTDPAKPNTAAFEKTLGEWKA
                     KK"
     gene            68757..69251
                     /locus_tag="PLP50_01530"
     CDS             68757..69251
                     /locus_tag="PLP50_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPA50256.1"
                     /translation="MDETPGSLRSPAIAHRLEREQVLPGMPEEVFPFFADARNLEALT
                     PPFLGFRILTPLPIEMREGTLIDYSLSLFGFPVRWKTRISVWEPGVRFVDEQLSGPYA
                     LWRHTHSFERRGAGTLMRDVVEYREPLGPLGTLAHRLFVARTVERIFDYRREAVVGLL
                     QRVA"
     gene            69248..69688
                     /locus_tag="PLP50_01535"
     CDS             69248..69688
                     /locus_tag="PLP50_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HPA50257.1"
                     /translation="MSAPAADLELFYDGACPLCAREARALRRLDRRGRLKLTDLAAPG
                     FDPAAAGVPMERLVERIHGRLPDGTTVEGVEVFRRAYTAIGFGPLVALTRLPGVSGLL
                     DLAYAWFARNRLRLTGRCEADACGRGVPTASRGQRRRRSTGSAT"
     gene            complement(69636..70211)
                     /locus_tag="PLP50_01540"
     CDS             complement(69636..70211)
                     /locus_tag="PLP50_01540"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPA50258.1"
                     /translation="MSSKARRTLRLPILALAALLASGVSLADEKAAKPATPPTRDELV
                     AAARKVMTSQTYCALITLDASGRPSVRTMNPFPPEEDLTVWFATNDRSRKVAEMRSDP
                     RVTLYYADHAKASGYVSLSGRAVLVDDRAEILKRKRAYWDQSFPGLKNLVLVKVVPER
                     LDVLNYEGKIVVDPVTWRTPSIDVGAVPSTP"
     gene            complement(70299..72011)
                     /locus_tag="PLP50_01545"
     CDS             complement(70299..72011)
                     /locus_tag="PLP50_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPA50259.1"
                     /translation="MKGLDWAVLGASLLFIVGYGTWRSRKQKGLEGYLLAGRSQAWWT
                     VALSIMATQASAITFLSTPGQAFADGMRFVQFYFGLPIAMVVLCVTVVPLFHRLKVYT
                     AYEYLEGRFDAKTRTLTASLFLLQRGLACGLTIYAPALVLSVLLGWSIQVTTLLIGGL
                     VVVYTATGGAKAVNETQTWQMVVILLAMGFALAALLRVLPEGVGFGDAVLVSGALGRM
                     NAVETTFDLGDRYNLWSGLIGGAFLALSYFGTDQSQVQRYLTGRSVAQSRLGLLANGL
                     VKVPMQFAILFVGAMVFAFYQFVAPPLWFNPVEAKKAAEARGAEMAALETAHAAAFEE
                     RRTEALRLVAAGKSGDETALVAAKESLRAAHARSEAVKAKAAAVVKEAVPKGEGKDTN
                     YVFLRFVLEVLPTGVVGLVLAAIFAASMSSTAAELNALSTTSVVDVYRRYFGKDVAEE
                     NLVTISKGLTIAWGAFAIVFAQYAGRLGTLVEAVNILGSFFYGTILGVFLTAFYLPKV
                     KGGAAFWGALAGEAAVVGAWLLTDVSFLWLNALGCAVVVAVALGFSAVRGTPDGESRE
                     TASA"
     gene            complement(72008..72142)
                     /locus_tag="PLP50_01550"
     CDS             complement(72008..72142)
                     /locus_tag="PLP50_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50260.1"
                     /translation="MADERPVADEPPPVGKRWGVLYAVVLGALALEVLLFWLFAESFR
                     "
     gene            complement(72135..74738)
                     /locus_tag="PLP50_01555"
     CDS             complement(72135..74738)
                     /locus_tag="PLP50_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPA50261.1"
                     /translation="MTRRSARPGAPLAVAFLALAAVAAASTAAAVPFGPERPAPAGEL
                     LLRLQKLNVLGSVLYVAAHPDDENTAMIAAFAEGRQMRTAYLSVTRGDGGQNLIGAEL
                     GPALGLVRTHELLAARRIDGGEQMFTRAIDFGYSKTADETFRVWGREEVLRDVVRAIR
                     TFRPDVVLARFPENGEGGHGHHTASAILAREAFRAAGDATRFPEQIEEGLSPWRPRRL
                     FWNGWRRDGKPIEGSIPYEIGDYSPALGRSYTETAAESRSQHKSQGFGSSERRGRLAN
                     ALVLLEGDAVTSDPFEGIDTTWGRVPGGTRVGRLLEEAERLFDAREPARVLPKLLEAH
                     AALREVGADPWVEVKRRELLAVIRSASGLWLEAIAASPSATPGSAFRLNVAALVRAGA
                     PATLARVVVPWGTGEGLPSGAELKLNEPVSGSASVPVPVDAEATQPHWLRARPARGLY
                     AVPEPKDAIRPDGASLRVSFDVVMAGETLTYEEPVFFRRTDPVKGEEIRPFVVEPPVS
                     VEIEEEVLVLPDHAPRVVKVTVRAAEVPKDRSGPPPLTPSIVASATGADGQAGEAPPP
                     KVDGAVRLSVPAGWRVEPAVRPFALAPGASATFEFTLRSPETPAVATIGAEAATGGDS
                     WSLTKRRIEYPHLPPLTYFERAEARAVHVDLARGRSRVGYVSGPGDEVPGVLRQMGYA
                     VTLLSADDLLRGDLARFDAVVLGIRAANTRSELKDAMPRLFDWVEAGGTLVVQYNTSF
                     EQVTDRLGPFPLKLGRDRVTDEESKVTLLAPEHPLLTTPNRIGPADFEGWVQERGLYF
                     PATWDERYVPLLGMADPGEKESKGALLAARHGKGTYVYTGLSFFRQLPAGVPGAIRLF
                     ANLVSGGTPRG"
     gene            complement(74818..>75917)
                     /locus_tag="PLP50_01560"
     CDS             complement(74818..>75917)
                     /locus_tag="PLP50_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HPA50262.1"
                     /translation="SLAWWVTEMHVDGFRFDLASILGRGRDGSVLTNPPLLERIAADP
                     VLANTKLIAEAWDAAGLYQVGHFPNWGRWAEWNGRYRDDLRRFLRGDGGMVPLLATRL
                     SGSADLYQGGGRAPHHSINFVTSHDGFPLADLFRYSHKYNEANGEGNRDGGDDGNSWN
                     CGHEGPGGPPEVDRLRRRMARNAFALLLVSQGVPMILAGDEAGRTQGGNNNAYCQDNE
                     VSWLDWRLVEENADLVRFVRTLIRFRKGSPLLRRRSFVPEGPGSAPVTWHGHVPFRPD
                     WSPGSTCLGMQLKDPAQGDDVFVVANAHHEAHRIELPPASEGRRWHVVADTYREPPAD
                     VFEEGTEPLVADPLRVEVGPRSVVILTGKRA"
BASE COUNT        10735 a        27763 c        27272 g        10147 t
ORIGIN      
        1 cctcgaccgt tcggcagaag ccgtcctcga cgacgaccgt ccagtcgctc cgcgcggcgt
       61 cgcggaaacg gaaatggaac cgggcgttcc acccctgtgc gcggtcggag cggaagcgga
      121 gcgggagcgt ctcgaacagc tcctcgacgc cccgcgggac cgggagcttc atcgcgatgg
      181 aggtcgagcc gatctccggg gtggggcgag cgggcgaagg cggcgcggac ggctccggga
      241 agtaggcggg ctccgtcacc tcgacgtccg cggggggcgg cgccggaggc ggagggacga
      301 acgacgggac cgtcgcctcg acggcgggcg ccggaggctc gggcgaggcc ggggacggga
      361 cttccgcagc ggcgcgcccc gggtcggagc cggcgggggg cggcgggacc tcggcgagaa
      421 ccgcgtcgag cgtctcggtg acgtcggcga cgaagaggtc cgggacgagg tcgcccgcga
      481 atgcgtcggt atcggcgtcg gcttcggaat ccccgcccgc gggagcgccc tccgcccccg
      541 cctcggcctg cgcgtcccgg cgctccgccc gacccgggaa agtgacaccg tcgatcgcgg
      601 acttcgcgat cacctcccgc atgatctcgc tcgtcccggc gacgatcgtc ccgaagcgcg
      661 cgtcgcggaa gaagcgctcc atcgggtact cctcgaccgt gccgtagccg ccgaagaact
      721 ggaggcactc gtccgccgtc cgcttcgaga gctccgtggc gaggagcttg gccatgcacg
      781 cctcggcgac ggggtcctcg ccccgctcgt ggagccaggc ggcgtggtag acgagctgtg
      841 cggccgcctc gagctcggcg tggaggtcgg cgaggcggtg gcggatcgcc tggaacttcc
      901 cgatcggctt gccgaacgcc tcccgcgaga ggacgtactt ccgcgtctcc tcgagggcga
      961 ggcgggcgct tccgaccgac gtggccgccg tgacgagccg ctcgaggacg aacgtctcca
     1021 tgatcaggaa gaacccctcg ttctccgcgc cgatgaggtt gccgagcggg acgcggacgt
     1081 ccgtgaaggt cagctcggcc gtgtcggagc agtgccatcc catcttccgg aggcgcgaac
     1141 gcgagaagcc gggcgtgtcg gcctcgacga cgagaaggct gatccctccg gcgccggcgt
     1201 ccgcggcggt ccggcacgcg acgacgacga agtcgccttc gacgccgttc gtgatccagg
     1261 tcttcgcgcc gtcgatcacc caggcgtcgc cgtcgcgccg cgccgtcgtc cggatcgcgg
     1321 cgacgtcgga gccggtatcg ggctcggaga tcgcgatcgc gccgaccttc gtcccggcga
     1381 ccgacggggc gagccatcgg tgcttctgct cctcggtgcc gtggagggcg atcgcccccg
     1441 tcgcgatgaa ctgctgcacc gagacggccg cgacgaagcc tcccatacgc gaccgcggca
     1501 gctcctcgag gagcgcgatc gcgtggaaga ccgagccgtc cgccccgccg agctcctcgg
     1561 gggcgaggac gccgaggaag ccgaggtcgc ccatgcgccg gaagacgtcg cgcgggatcc
     1621 gccgcgcttc ctcccactcg cgcgcgcgcg gcgcgacctc accctcgagg aactggcgga
     1681 ccgagcggcg aaagctctcg tgctcctcgc cgaagtagat cgacgtcatg cgtaccctcg
     1741 ggatggaata atcgggccgg ccccggaacg aagagattac gcctgtgccc gacaccctgc
     1801 ccgactcccg gacgcctgcc gcggcccctt ccggatcctg gaagctcgag gaggcctgcg
     1861 ccgcacccgg ccgcggggcg gaggagctcg ggatcgcgtg agctcccgcc gggacttcct
     1921 cgggagcgcc tgggcggcgg ccggcgtggc cgcggccgcc gccggaacgg ccgtcctcgg
     1981 ccgggccctc tccgggtcgc ccgagcccga gaggtacgtc cgcctccccg ccgagctcct
     2041 cgcccgcgcc gagaaggagg gggggctggt cctcgaaggg ctccacgtcc gcggccccgc
     2101 ggcctccccc gtcgtcctcg acctcacctg cacgcacctg cgctgccgcg tggctccgat
     2161 cgaagggggc ggcttcgcct gtccgtgcca cgccagccgg ttcgatggcg aagggcaccc
     2221 gacgtcgggc cccgcgaaga gaccgctctc ccggccgcgc ctctcgcccg acggcgccgg
     2281 cttcgtggcg cgcctctcgt gatcctcggc cgcctcacgt tcggcctcct cgcgatcgcc
     2341 gtcctctccg gcctcgccct cgccccgttc tggtcggccg cgcgcccgct cgagagcctc
     2401 gaggcgctga tcggggcgac cccgttgggc ttctggctgc gcggcgtcca cgccttctcg
     2461 gcgtgggggt tcctcctcgg gaccgtcgct cacctggcgg aggtcctcgc gaagaggacc
     2521 gagcgacgcc ttcggccgat cgtctggtgg cgctccgtcg ccctgctgcc gatcgctgtc
     2581 ctcggcctcc tcggcggttt cctcatgcgg ggcgacgccg aagctcaggc ggcgctcgcg
     2641 gtcttccggg aggtctcggc gaccgtcccg atcgccggac gggcgctcgg cgcgctcctc
     2701 ctcggcgtcc ccggcagcga cctggcgccg gtcgcgctgc accacgccgg caccttcacc
     2761 ctcctcgcct ggctcctgac cgccgagcac ggagggacgc cgttccccga ccttcgcgcg
     2821 acggtcctcg ccgcgctcgg gagcttcgcg gccgcgggcc tctttcccgt ccccctcggg
     2881 ccgccggtcg cgccggacgg agaccttctc ctcggcccct ggtacctgct cggcctccag
     2941 ggagccctcc tcgacctccc cgtcgcggcc ggctggctcc tgccgctcgc cctcctcctc
     3001 gcggtcgggc ttgcccggca cgtcgacggg cgcgcccggg cggccctcct cgcgctcgcg
     3061 gccgcctggg gtgcgggcta tctcgcactg acgctccgcc tcctcctcgc cgggcgctga
     3121 tgttccgggc gcaggcgctt ctcctcgcgc tcctcctcgc gctcgcgggg ctgctcgggg
     3181 cgctcggccg ggaacggcag cgggccgccg ccgtcggacg cggtcccgac cgcgccgggg
     3241 ggaaggtgga gcgctgcgtg acgtgccacg tccgccccga ggaggacccc ggcggggcgc
     3301 acgcccgggc cgccgtcggc tgctcctcct gccacctcgg caacccgctc gcgttcgata
     3361 aaacgcgcgc gcacgccggg atggagccgg agccgggcgc gcaccggagc gtgacgcgca
     3421 cgtgcggccg gagcggctgc cacgtccgga acgtcctcgg cgagagggaa cgcgcaatcc
     3481 acgggaacgg agacggctgc gtcgcctgtc acgtcgcgcg ccgcctaccg ggctcggtcc
     3541 cgcggccgca cccgggggtg gacgcgcggg tcaccgacga ccggtgcctc gcctgccacg
     3601 ctcctcccgc cgggacgcac ggggagctcg ccgagacatc gccggcgggc tccggcagcg
     3661 cgtcggcgcc tgccgacgcg ccgtcggcct gccgcgccgg cgccgacggg catcgcttcg
     3721 ccggcctctc ctgcatcgac tgccacctcc acgccgagct gatgggcgac ggagcggcga
     3781 aggagacgag ccgggtggag atcacgtgcg aggcgtgcca cggacccgtc cgagaagggg
     3841 gcgagacgac ctggtccgag atccgggacc ctgtcagccg cgaccttctg cgccaacggg
     3901 gcgagagccg cgagccttcc gaacgcgtcc gcctcgggcg acgcggcacg ccgctctgga
     3961 acctccggcc gtccgacgac ggctggacga cgacgcgaaa ggggcacggc gtcgccctcg
     4021 cgacgccttc cacgccggtc gacgccagac acgacctccg ggatggaggg cgtccggcgt
     4081 gcgtttcctg ccacgccgtc cgggcgccgg tcatcccggc cctgtgaaga gggcggaccc
     4141 ggtttggatg ggcttcgtcg ggacgccccg cgctcgcggc gacgaacggg cgtccctgtg
     4201 cccccacctg gtctcagcag agaggacagg gcatgtcggc ctgccggact ttcgctgccg
     4261 acggtggagc cccgcacttg tccttcaggc acgtgttcag gtggaagccg aggagctccc
     4321 gggcccaccg gacgtggtcc tcgcgcaacg tcgccttctc gtccttcttc cagccgcacc
     4381 tcccggccct ctcgttctcg aggcagcgga acgtgaggtt cccgagggcg taggcgaagc
     4441 cgaggaggag ctgctgtttc gtcgagtcga gcgtggggta gatccggtcg aagcgctcga
     4501 ggtagacgtc ctccagggcc ttccgggaga gcttcgcctg cgctgcgtcg agctcgccgt
     4561 acccgagccg gaactcctcg aacgcggcat cccggtcctt cgcgaggtcg ccggtcgcct
     4621 gcgccccgtt cgggggcggg gcgcccaggc cttcctcggc gagcgatgcc aggaatgcag
     4681 aaccggccgc ggtcagcagc caacgcctgt cgaacggcat gtcgtcctcc tcgtaccgag
     4741 tgggtcggat tgaccggata atcctcgcgc ggaatgacct cagtcaagcc ggttctctcg
     4801 cggcttctcc gcgcctacgc cgtatggggc gcgttgctcg tcttcgcggg ccttccgagc
     4861 gcgctgtaca gactcgaaac ggtccggaat ccgccggtcg tcctcgagct gttgccacac
     4921 gccgacggat cggtcgaagg gaccgtcgcc gccgacgggc gacggacccg gatcacgcac
     4981 gtctccgtga agggcggccc atggctcgcg gccggaggcg tcgccgccgc gaggacgctc
     5041 tgggacctgc cggccgggac gcccgtcgcg tatcgcgaag cacgcgcggg cgaggcgccg
     5101 tccgtcgtga gcggcaccgt ctccgagcac ggcgcgacga cggccgaagc cgtcgcggcg
     5161 gccacgctcg cgctgctcgc cgtcgtcctc gcggcgtccg gcgccgcgat cgccgtcgcc
     5221 ggcgtcagct cgcaggcgct cctctccggg gcggccctgg ccggcctcgg gcacttcgcg
     5281 gcggctcgct tcctcgaagc cggtgcctcg ctcgtcctcg atcccgcgac gcgcggagcg
     5341 ctcgtcctgg gctgggtcgc gttcccccgg catttcgcct tcctctctct cgccgcgttc
     5401 ctgtccgtct tcccgtccga cctcaccgcc cggcggctct gccggctcct cctcgcgttc
     5461 gtcggctcga tcgcgctcgc gcaggccgtc ctcgtccccc tgatccagct cgaagggccg
     5521 ctcgacgggt tgagcgtggc gacgcagggg cggatcgtcg cctggacccg gcagctgacg
     5581 gccctcctct tcgggaccgc ctcgctcagc tcgctcgtcc tgatcgggat gcaggtacgc
     5641 gcgttccggc gggagccgat cgacgtggcg acgcggcggc gcgccaccgt cgtgggcgcc
     5701 ggcctcgtcg ccggcctcgg accgccgctc ctcctcgcgt tcgtgcagat cgccgcgagc
     5761 cgcctcgtcg gacggcctct cctcccgccg gccaccatgg tcctctccct cctgccgatc
     5821 ctcctcgtcc ctcccgccct cgcgtacgcg atgctctcgc cccgggtcgt ctcggtcggg
     5881 ctcttcgcga ggaaggccct tctcctcggc ttcgcgcagg ggacggtccg cgccgcgtcg
     5941 ctcgcgcccc tcgccgctct cggtctcctg ttctacacgc ggagggacga gtcgatcggc
     6001 cacgtgttcg gcgaccacct ccccttcatc ttcctcgcgc tcgcggtcgc gacggccggg
     6061 ctgcgctacg gcggccggac gcgggacctc ctcgagcgcg ccttcttcgg cacacggaag
     6121 gcctccgccg ggacgctcgc cgacctcgcc gccgcgaccc gccgcgcgct ggacgcctcc
     6181 gagctctcga ccgccctcgt ggaggggata cagaaggccc tcggcctcga gcaggtcgtg
     6241 ctcttcgtgc gggaggagct ctccggcacg ttcacgtgcc ccggccgtcc cctgccgccc
     6301 ctcgacggct cctccccgat cgtggagctc gcgggcaagc gggccggccc gttccgcccc
     6361 acggcggacg gcgacccgga cgcgccggcg gcgctcacgg aggtcgaccg gcagtggctc
     6421 gactccaccc gcgcccgcct cctcgtcccg ctccgggatg cttcgggaac gctgatcgcg
     6481 ctcctcgccg tcgccgacaa gcagagcgag ctcccgttcg actccgagga cgagcagttc
     6541 ctcgcggccg cggccgcagc gggcgccctc acgctggaga acctccagct ccgctcctcg
     6601 agctcctccc ggtccgcagg gggccgcccg gccgacgccc ctctcacgca ggccgagacg
     6661 gatcccgagg cggcgcgcct ctgccgccgc tgcgcccggc tcttcgctcc cgagacgggc
     6721 ggcacctgcc cggacgacga gacgttcctg gagccggcac cggtgccggt gctcctcgcg
     6781 gggaagtacc ggctcgtgcg tcggctcggc gccggcggga tgggcgtcgt ctacctcgcg
     6841 cgcgacgtcg ccctggcgcg ggacgtcgcg gtcaagacgc tcccgagcct cgccgccggg
     6901 agcgtccgcc gtctccagcg cgaggcgcgg gccgcggcgc tcctcgtcca ccccaacctc
     6961 ggcctcatct tctcgtcgga gagctggcgc gggacgccga tgctcgtcct cgagtacctc
     7021 ccgggaggga cgctctccga ccggatccag cagggcccgg tctctcccgc gtgcgccgcg
     7081 gcgtggggca ccgccctcgc ctccgccctc gaggcgatcc acgcgcggaa cgtccttcat
     7141 cgcgacgtga agccctcgaa cgtcgggttc gacgccgcgg ggacaccgaa gctcctcgac
     7201 ttcgggctgg tccgcctcct cgacgaggtc gaggccgcgc cgatacccgg cctcccgccg
     7261 gaggttcccg agagccacgg ttcctcggtt tcggcgaccc gaacttcggc gagccacgtg
     7321 gtcggcacca tcccctacct ctcccccgag gcgctccagg gccggccgcc cacgccgcgc
     7381 ttcgacctct gggggctcgc cgtgacgatc tacgagtccg tcaccggcgt gaaccccttc
     7441 gccgccacga gcaattcccg gacagtggac aagatcctga cccgcagcgc gccggacccg
     7501 cggacgatcc ggcccgactg ccccggtccc ctcgcggagc tcctgctcgc ggcgctcgcg
     7561 cgaaccgaag cgaaacgccc cgcctcggcc cgcgagctcc gggagggctt cgaggccgca
     7621 gcgcgggggc tctcagaagc tcctccggat ctcgaccgcc ctgcgcccgc gggcgacccg
     7681 caccgcgcac cgaccggagg ccgatagcgc ccgacgcccg gtcgggcctc cgcgaggacg
     7741 gcgtgaacct cgtccatccc gctgacgtgg atgagcgcac tcgcacgacc gctgccgagg
     7801 ttcgtgacgc gaaacccaac agccacgcgc agggcacgcg cggggagcga gagacgcctt
     7861 gctgcactga cccttctggc gctcgtccgc tcccccgggc acgaagcaga gcttggtggc
     7921 ggctcaggaa ccctctatgc tggaccaggc tccgctctcc atccgccccg gccgcgacga
     7981 ggagtccgca tgaccgaacg cagcacgccg cccccggagg gctccgcgac ggtcggcgcg
     8041 cgcggcaccg tgccgccgga gctcacggag accgggaagc tccgcatcct cacggcgcag
     8101 gagatcgagg cggcgaggag cgagacggcg cggacccagg aggcgctgaa ggagagcgag
     8161 gcgcgcttcc ggaccctctt cgagagcagc cccaacggga tcttcatcac cgacccggag
     8221 accctcgaga tcctcgactg caacgcccag gcgtgcgaga tgaacggcta cgcccgcgag
     8281 gagctcgtcg gccggagcat caacgacctc caccccgagg aggtccgcgc gcagacgaag
     8341 ggggggccgg aaggacgcgc ccgcttcgtc gagcagctgc gggagaaagg ggtcatcacg
     8401 gtcgagtcgg tccacgtccg caaggacggc acgacattcc cgatggagac gtcgatgtgc
     8461 ctcctcgagc tcgggggtcg cggcgtcgtc atggggatcg accgggacat cagcgaacgg
     8521 aggagggcgg aggaggagct cgcgcgccac cgcgagcacc tcgaggagct cgtggcctcg
     8581 cgcaccgccg agctcgcggt cgagagggac cgggccgagg cggccgaccg gctgaagtcg
     8641 gcgttcctcg ccacgatgtc gcacgagctc cggacgcccc tcaactcgat catcggcttc
     8701 acgggcgtcc tcctgaaggg gctcggcggc ccgctcacgg ccgagcagtc ccgccagctc
     8761 gggatggtga agtcgagcgc gcagcacctc ctcgccctca tcaacgacgt cctcgacctc
     8821 tccaagatcg aggcgggcca gctccggatg tcgttcgcgc cgttcgtgct gaaggacgcc
     8881 gtcgaggggt gcgtccgcgc cctggcgcca gccgcggaaa agaagggcct cgtcctcgcg
     8941 gcgaaggtcg accctgccag cgagacgatc gtgagcgacc ggcggcgcgt ggagcaggtc
     9001 ctcctgaacc tcgtctcgaa cgcggtgaag ttcaccgagg ccgggagcgt gacggtgagc
     9061 gccgcccgcg agggggcgtg ggtcgtcacc cgcgtccggg acaccggcat cggcatcgcc
     9121 gccgaggacc tcccgaacct gttccggcct ttccagcagc tcgagtccgg cctcacccgt
     9181 cgcttcgaag ggaccggcct cgggctctcg atctgccggc ggctcgtcga gatgctcggg
     9241 ggggagatcg ccgtcgaaag cgctcccggc cgggggagca cgttcagctt ccggcttccc
     9301 gccggagagg cgccggccga gatcggcccg gaggggtgat gagcggaaac gtcctggtcg
     9361 tcgaggacaa cgagcagaac ctctacctcg tccgtttcct gctcgaggga gccggatgga
     9421 ccgtcgtcgc ggcgccggac ggcccgcggg cgctcgagga agcgcggtcc ggccgcttcg
     9481 atctcgtcct cctcgacatc cagctccccg gaatggacgg ccacgccgtc gcgcgcgagc
     9541 tgcgacggat ccccgcgctc gacggcgtcc ccatcgtggc cgtcacgtcg tacgcgatgc
     9601 tcggcgaccg cgagcggtgc ctcgaggccg gctgtaccgg ctacgtcgag aagccgatca
     9661 atcccgacac cttcctcgac gaggtggcga gctacgtgcg gccggaggtc cgtccgtgac
     9721 ccgcgtcctc gtcgtggacg acgagtcgca gaaccgatac ctcctggagg tcctcctcgg
     9781 cggcgaggga tacgaggtgg tgtcggcctc gaacggccgc gaggccctcg cggcggcccg
     9841 gcgtcgcgcg ccggagctcg tcatctccga catcctgatg cccgagatgg acggcttcct
     9901 cctctgccgc gcgatgaaag cagacgcgac gcttcggaac gtgccgttcg tcttctacac
     9961 ggcgacctac accgaggccg aggacgaacg cgtcgctctc gaagcgggcg gggacctctt
    10021 cctccggaag ccgaccgagc cggataccct gctcgccgcg atccggcggc tcctcgagga
    10081 gaggatcccg gtgagcgacg aggggccgac gcgaggcctc gcggaggagg tcgccttcct
    10141 cgcgcgtcac gaccaggccc tcgcacggaa gctcgagaag aagcaccatg agctgcagga
    10201 gagcgaggcc cgctatcgga gctacttcag gagctcgccg gtggcgatcc tggtgacgga
    10261 cgacacggga cggctgctcg acgcgaaccc cgcggcgggc ctcctctccg ggcgcgacgc
    10321 cgtggagctg ctcggcgcga acgtctcgct cctgctcggc gaggggggcg acgactcgcc
    10381 ccttcgcctc gcggcgggcg cgccgtccgc cccctcccgg acgaccgcgt ggcgcctccg
    10441 gcgaaaggac ggcgccctcc ggcacgtctc cctcacggcg atccggctgg aggacgggcg
    10501 ggtcctggcg ttctgcgagg acgacaccga gcgggtccgc gcggaggagg cagtccgcca
    10561 ggtgaacgcc gacctcgaac ggagggtccg ggagaggacc gcgcagctcg agcacctcaa
    10621 cgccgagctc gaggccttca cctcctcggt gtcgcacgac ctgcgggcgc cgctccgagc
    10681 gctcgacggc tacgcggcga tgctcgagga ggaggcggaa cgcctcgacg aagacggccg
    10741 ccggcacctc ggcgcgatcc ggcggaacgt ccgccggatg tccgagctcg tcgaaagtct
    10801 cctctccctc tcgagggccg gacgccagcc gctccggacg ggaccgatcg acatggagcg
    10861 actcgcccgc tccgtcctcg acgagctgct cgtccccggg gagcgagaga ggaccgaggt
    10921 cgtcttccgc ccgctccccg cggccgtcgg cgacgagagc ctcgtccgcc aggtgctcgc
    10981 gaacgtcgtc gggaacgccc tgaagttcag cacacggaag gagcgccggc gtctcgagat
    11041 cggcgcccga aacgagggag gtcggacttt ctggttcgtc tcggacgacg gcatgggctt
    11101 cgagcccgcg caggcggaga ggatcttccg gatcttcgaa aggctccccg gcgccgagga
    11161 tatcgagggc accggggtcg ggctcgccgt cgtgcggcgg atcgtcgaac gccacggggg
    11221 cgtcgtccgg gccgagggaa cgcccggggc aggcgcgacg atctcgttct cgctggggga
    11281 aggcgccggc tgaggggcgt tcagctcgga agagccttct cgatcgcggc cgcgaggctc
    11341 tccggggcgt cgttcggggc gaagcgggtc accggcgcgc cccccggggc gacgaggaac
    11401 ttcgtgaagt tccacttgac cgcctcggtc ccgagcaggc ccggagccgc cttcttcagg
    11461 tgccggaaga gcgggtgggc gccgtccccg ttcacctcca ccttcgcgaa catcgggaac
    11521 gtcacgccgt aggtccgcga gcagaaggcc tggatctcct cctcgcttcc cggctcctgg
    11581 tgaccgaact ggtcgcacgg gaagccgagg acgacgaggc cccggtcgcg gtacctcttc
    11641 cagagcgcct cgagccccgc gtactgcggc gtgaagccgc acttgctcgc gacgttcacg
    11701 acgaggagcg ccttccccgc gtgcttcgcg agcgtctcgg ccttcccgtc gatcgtccgg
    11761 acctcgatgc cgtcgagaga tcccatgcca ctcctccgtc gcctcaccta cgcccccggc
    11821 cggaaatcgg atcgcggccc ggaacgaggg cgccgcccgc cctcagggga gcttcttgtg
    11881 cgtcccgtcg cagaacgggg cgttcccggt gtgcttgcac tggcagagcc acgcctcctt
    11941 cgcctcctcg gccttccaga cgaggggagc gagctccgtc ccccggtgcg agccgtcgca
    12001 gaacggctgg ctcttcgaga gcccgcaacg gcaccaggcg tacgtcttcc ccgcctcgag
    12061 gctcacgcgg acgggctcct tcgcggcgat cttcggctcg gacatcggcg tcctcctcgg
    12121 cgtagcggtc gtcgtcgcga catcatagcg tcgcgctcga ccgtcttcgc acgccgagtc
    12181 agaacgccag gttcagctcc gcgcggacga aggacgactc gtcccttcgg tgagcggcgt
    12241 agaagtcgcc cgggcggaag tactccgcgt gggggtgcgc cgagacgtgc gcgtcgaacg
    12301 cctcgtcgcg gccgctcgcc cggtcgtcac ccgagaggtg ctcgaacccc aggtgtgccc
    12361 ggtgcctccg tccccggccg aaccgccgcg cgagccgccc cgtgaacccg aaggctcgga
    12421 ggtcgcggcc gtcgcgccga cccctctggg ctgcgccctc ggcccggagc tcccagcccg
    12481 tggccggcac gacctcggca cgccctccga gcgtgaggag ctctcccgag tcggaaggag
    12541 atgggaaagg agctccgttg ttcacccgga cgttccccgg agcggccttc gtacgtgcgt
    12601 ccttccagac gacgtacccg tcgagcttcg cccccttcac gagcgactcg tcgcgcgccc
    12661 agaggatcgc ccctcgctcg ccctgctcga gctggtcttc gctcccgccg aggagcgggc
    12721 gcgggaatcg cccggcgcgt cccgcgtgct aggttctcgc cgtgcgggcg agggcatcgt
    12781 cccctccgcc acaatcagga ggtcgaccat ggaatcacgc actctcggaa cgcagggcct
    12841 cgtcgtctcc gcccaggggc tcggctgcat ggggatgtcg gagttctacg cggggcgcga
    12901 cgacgccgag tcggtcgcga cgatccaccg cgcgctcgac ctcggcgtca cgttcctcga
    12961 cacggccgac atgtacggcc ctcacacgaa tgaggtcctc gtcggcacgg cgatccgcga
    13021 aaggcggagc gaggtcgtcc tcgcgactaa gttcgggaac atgcgcgggc ccgacggcgc
    13081 cttcctgggc gtcaacggcc ggcccgagta cgtccgggcc tgctgcgaag gctccctcgc
    13141 gcggctcggg gtcgaggtga tcgacctcta ctaccagcac cgcgtcgaca agtccgtccc
    13201 gatcgaggag accgtcggcg cgatggccga cctcgtgaaa gaggggaagg tccgtttcct
    13261 cgggctctcc gaggcctccc ccgccacgat ccgccgcgcg cacgccgtcc acccggtctc
    13321 cgccctccag accgagtact ccctctggac ccgcgacccg gaggacggcc tcctcgcgac
    13381 ctgccgcgag ctcggcatcg ggttcgtcgc ctacagcccg ctcggtcgcg gatttctcac
    13441 ggggcgcttc cggcgcttcg aggacctccc cgaaggggac taccgccgcc tcgccccgcg
    13501 cttccaggga gagaacttcc agaagaacct cgacgtcctc gcgaaggtcg aggagatctc
    13561 gcgcgagaag ggctgcacgc cggcgcagct cgccctcgcc tggctccacg cgcagggcaa
    13621 cgacgtcgtc ccgatccccg gcacgaagag ccgcgcccgc ctcgaggaga acgtcggcgc
    13681 gctccgggtc tcgctctctc cggccgacct cgcgcggatc gaggccgtcg ccccgaaggg
    13741 cttcaccgcg ggcccgcgct acccggagat ggcgatgagg gcgatcgacg cctgaggcgc
    13801 gcggctcacc cgagccgagc ggcctctcgc tccctcttcc ctccgtcgcg ccatcggaag
    13861 tgccgggcga gctcggaggt gtaggcggta cccacctcgg cacggtgggc ccggagccac
    13921 tcggtgaacc gcgtcggcgc gttcggcgcc tcctccgacg tcagcttctc gtccatcagg
    13981 ccgcggagct cgtcgcgcgt gaggacgacg tcgcggagga ggaggccgac gaggcggccg
    14041 gcggcgatac cgagcgccgg ggggacgcgg acgatccgcg ccctcgatcc gacggcggag
    14101 gcgaggaggc ggacgagctc ctcgaacgtg aacgtctcgg ggccgacggc gtcgaacgtc
    14161 cgccccggct cgccgagcgc cgcctcgacg gccagctcgg cgaggtcccc cgcgaagacg
    14221 ggctggacac ggtaggaccc cgagccgaag agagggaaca cggggaacgt ccggaggagc
    14281 caggcgatgt tgttgacgag gaggtcttcc ctcccgaaca ccagcgtcgg gcggacgatc
    14341 gtgaacggga cgcccgacga tcggagcgcc tcctcctgcc tcgccttccc gcggtagtac
    14401 ggaagggggg agtcgaggga gcagcgagtg acgctgacct ggacgatccg ggagacgccc
    14461 gcctcgcgcg cgcaccggaa gagcgtcgag gtgtttcgca cggccttctc gaacgaggag
    14521 tcgccgtggt cgaacctcac ccagtaggtg ttgtagaggg tcgtcgcgcc gcggaggctc
    14581 tccgtgagcg ccgccgggtc gtcgaaggag aaggggaacg ccgcgacggc cgggccgaac
    14641 gggttcggct tgtcgacgtg cgtcgtgatc gtccgcacgc tctcgccgcg ggccaggagc
    14701 gcacgcgcga cgtaccggcc gatgtagccg aaggctcccg tgacgacctg catggagggc
    14761 cggatgatgc catcccgcgc cggccccgcg gctctcgccg gaaaccggcg cccctcctcc
    14821 ggcatcggag ccgtgaagga gatccgatga tccgccgcct gatcgccgcc cgctcgttcg
    14881 tcctccgcgc ctcctcctct ctcgcgtggc tcccgctcgc cgccacacgc gtcgcgctcg
    14941 ggacggtctt cgtccagtcg ggctggggga agctgcacga cctgccgcgc gtcgtcggct
    15001 acttcgagag cctcggcatc ccggccccgc agctgcaggc gccattcgtg gcgggcgtcg
    15061 agctcgccgg gggcgcgctc ctcctcggcg ggctcttcac gcgcgtcgcc tccgtcccgc
    15121 tcgcggcgac gatggtcgtc gccctcctga ccgcgaggcg cgccgacctc gcgaccgcct
    15181 ccgacctctt cggcgcggtc gagctcctct acctcctcct cctcggctcg ctcgcggcgt
    15241 tcggggcgga ggcgctctcg ctcgacgcgc tcctcgcccg acgcttcgcg ccggacgagg
    15301 agctgaaggg ttagctcgca gcgctcgggc cgggggcgac cccggcccga gcgaaggcgc
    15361 cgagcgcgct ccactccgcg agccagcccg agacgacccg ggccggcggg cgcctgcgcg
    15421 ccgcgcgcgc gagcgcttcg ccgagcggga agcccgagac gagagccgcg aggagccggc
    15481 ccgacaccgg gtctagggcg cggcgaagga cggtgaagtc cctccggtgg aaggcgacgt
    15541 agaccagacc tcgcccggca gaggtcggga ccggagcgtc gtctaggacg gcgtcgagga
    15601 ccgccacagc gctgggccgt acggcgagga tcgcgaaggc gggcgcggcg aggaggcgga
    15661 gcgactcccc ctccgacgcg agcgcggccg ccagcgcggg ggcatcgagg agcgcgacgt
    15721 cggcggcgtc gaagacggcc gccgcggcgc gctcgaggcg ggcgacgtcc gcgcgaaggc
    15781 cctgcccgcg cttcggcccc cacgccgcga cgaacgacgg gagccggtcg cccagccggg
    15841 cgagcgtgaa gctcgtggag gggtgtgccg cgacgtactc ctgaacgagc gcgccgaacc
    15901 tctcctcgcc gaggagggac gcgagggccg ggtagtcgga gcggagcgcc tcgaccgtcc
    15961 gcagggggta catcgacgcg tagatcccga ggcgctcgac gggagagagc gctccgtgcg
    16021 cgcggacgac ggcgccgagc cgcgacggcg ggaggttctg ccgcgcggag agcgaacgga
    16081 ggcccgcgtc gacgccgccg ggatggacga tcaccgcctg catccactcc tggaaggtcg
    16141 cgagggggag gccggccgac gtcacgccgc ctcccgctcg ctgtgcccgt cgcggtcgcc
    16201 gagggcggcc tcggccacgg ctcgcgcctt ccgcgcctcg gcgaggacga cgtcgagcgc
    16261 cgggatctcc tcgtcccact cgacgagcgt cgtcacgggg ccggtgcggc ggacgagccg
    16321 cgcgtagaga gcccagacct cgtccctcac gtggtcggag tgcgtgtcga ggaggtgcgt
    16381 cccgaggtcg gtgtgccccg cgaggtggac ctggacgacg cggtcggccg ggatcgcgtc
    16441 gatccacgcc tcggcgtccc agccgtggtt gaaggcggag acgaagacgt tgttcacgtc
    16501 gacgaggagg ccgcacccgg agaggtcggc gaggcggccg aggaacacgg cctctcccat
    16561 cgtggaggag gcgagctcga ggtaggtgga ggggttctcg aggtggaccg gcctctcgag
    16621 gaggtcctgc acgacgcgga ggcgcgcggc gacgtgcgcg agcgtctcct cgttccgtgg
    16681 gagcggcagg aggtcgtgcg tgttgcggcc ggcgacgccc gtccagcaga ggtggtcggt
    16741 cacccagcgc gcgtcgacgc gccgggcgag cgccttcacc gcccggaggt agccgaggtc
    16801 gagcgggtcg acgctcccga cgttcatcga gactccgtgc aggacgacgg gcgtcctctc
    16861 cgcgagccgc tcgaggacgt ggaggggccg cccgccggtg tcgaggaagt tctcggtgat
    16921 cgcctcgacg aaatcggccg cgcccggccg ctcgaggtgg tcggcgaagt ggaccgtccg
    16981 gaggcccacg ccgtagccga gcatcggcag gccgaatcgg tttcgcatgt cgtcctccgg
    17041 tcgggcgggg cggccccgcg gcgcggggcc gccctcgcgc cattcgatca ggccggcttc
    17101 gcggtcggct gcgccgaggc cttgccgtcc gtccggcagc cgcccatccc cttgcaggcg
    17161 ttgtggccgc gacagacgtt cttctccgtc ttgcagcccc cctttccctt gcaggcattc
    17221 tgccccttgc aggcgtgccg gtcggtcgcc tcgggaccag ccttcttgtc gcccgtcttg
    17281 tcccccgcct tcttgccccc gcagccgttc gggccgccgc aggcgttctt gtccttctgc
    17341 gtcgcggccg gcgtccccga gggggcagga gtcttctcgt cggcgcggag cgtgcccgcc
    17401 gcgagggcgg agctcgcgac gaggccggcc acgagggcgg acaggtagac gttggacgga
    17461 cgattcctct tcatggtgtc tcctcttacg acaggcctcg cctgtcccgt tcgtttcggc
    17521 cggccgcgcg ggccggtccg caggtcactg cacccgggat gccggtccgc cgaaaaggtt
    17581 tcagctggca gaaacgaatc gatcgacggc ctccggaccg atccgttcgc gaaaccggag
    17641 cgtacctgcg agcatcaagg gagcgtgacg atgcccgaga cccccaccgc acccccgctc
    17701 cgatccgccc ccggccgatc ggggtccgcc gccgccctcc tcgccgccgg cctcgcgctc
    17761 gcggccggct tcgccgcctg cggcccgtcg cgctcgctcg ccgggcctcc ggccggaaag
    17821 gaaacgaact ccgagaaggt ggagaagatg aagacggtcc tctcgaagtc cggctacgac
    17881 gtcacgcccc tttcccgcga ggaggtcgcc cgcctcgcga agaagctctc cccggagtcg
    17941 taccgcgtga cgcagaaggc cgggacggaa gccccgttct gcgggaacct cctcgacaac
    18001 aagaaggagg ggacgtacct ctgcgtcgtc tgcggtctcc ccctcttctc gagcgagcac
    18061 aagttcaact cggggacggg ctggcccagc ttcttcaggg agttcgatcc gtcgcacgtg
    18121 acgcggacga tcgactcgtc tcacggcatg gagcgggtcg agatcaactg cgcccgctgc
    18181 ggcgcccacc tcgggcacgt cttcgaggac ggccccgcgc cgacgggcga gcgccactgc
    18241 ctgaactccg cctcgctgac gttcctcgag aagggggcgc ccgtcccccc ggagagccgc
    18301 cccgtgaagg ccgagacggc ctacttcgcc gccggctgct tctggggggt gcagcactgg
    18361 ttctcgcgcg ggccgggcgt cctcgaggcg acgagcggct acatgcaggg acggacggag
    18421 aagccgacgt accgtcaggt ctgcgaggga gacaccgggc acgccgagac ggtgaaggtc
    18481 gtctacgacg cgaaccgcat cacgtaccct cggctcgtcg aggcgttctt cgccatgcac
    18541 gacccgacgc agctgaaccg ccagggaccc gacgtcggcg accagtaccg ctcggggatc
    18601 tggacggcga acgacgagca gcggaaggtc gccgacgagg tcctcgccgg gctgcgcgag
    18661 agtaaggcgt acggcgagag gaagatcgtg acggtcgtcg agccggcgaa gacgttctgg
    18721 cccgccgagg agtaccacca ggagtacctc gcgaagaacg gcgccgcctg ccacgtgacg
    18781 aacccctggt aagcggggag cagactttcg agtgagccgc gcgtgaccaa ggaccggccg
    18841 cgatttccgt cacgacggtc cgggcggcta cgatccgcgc gttgtccgag aacgaggcac
    18901 gggagaggct ggcgaagctg atggtggcgt accagggcgg ggacgagtcc gcgttcgaga
    18961 gcctctacgg cgagctcgcg ggcccggtga gggcgttcct cgcctcgcta acgcggaacg
    19021 cggcccgcgc ggacgacctc ctccaggaga cgttcttcca cgtccaccgc gcgcggcaga
    19081 cgtacgaccc gacgcgctcc gccaaggcgt gggtctacgc gatcgcgcac aacgtcttcc
    19141 tgatggcctg ccgcactgac cggaggcgcc gccggaacga ggagctcccg gcggaagacc
    19201 tgcccgacct tcccgtcccg gccgaggccg agtcgctcgc ctcgagggac ctcgtctcgc
    19261 gcgccctcgc gacgctcccc gtcgaccgtc gcgaggcgct cctcctccac cacgtggaag
    19321 ggctctcctt ccaggaggtc ggggccgtcc tcggcgtcac gacgatggcc gcgaagctcc
    19381 gctcgcaccg cgccatgggg cagctccggg aactcctcgc ccgcgaggga gcgcgatgag
    19441 cccgaccccg ctcccgcccg cgctccgcgc cgccgtcgcg agcgacctcc ggccggtccg
    19501 cccgctcgcc tcgcccgcgc ggcgcgcgct cgccctcctc gcctgggccg ccgtcgccgc
    19561 cgccctcgtc ctcgccctcc ggggcctccg ctccgacgcc gccgagctcg gcctcctctt
    19621 gacgtggggc gtcgtcctcg cggaggtcgc cgcgggcgcc gggctcgtcg ccctcgctct
    19681 cgccgaggcc gttcccgcgc gggggccggg gcgaggcgcc gcgctcctcg ccctcggcgg
    19741 cgccgcggcc ctcttcgtcg cgctcgccgc cgtgacccgc cgcgcgagcc gcgggatgga
    19801 ggtcgacgac ccgctcctga gcttcggccc ctcctgcgcc ggcctgcagg gcttcatcgg
    19861 cctgacggcc ttcgccgtcg ccgccctcct cgtcctccgg gcggccccga tgcgcgcccg
    19921 actcgcgggg ctcctcgccg gcgccgggac cggcttcctc gccgagggcg tctaccacct
    19981 cgactgcccc atcacccacc tctcgcacgt cctcgtctgg cacggctccg ccatactcct
    20041 cctcgcgctc ctcggcttcg gcttcggcct tgccctcgag gcgagggagc gggcgcggat
    20101 ggaacgacgg cgcgccggca cggccgcgcg gaaaggaggc gagggatgag cgacgaggtc
    20161 ttccggaagc tcacgagcct ctcttcctgc gcggggtgag cgtcgaagct gccccccggg
    20221 cagaccgcgc aggtcctgcg cgtcctcggt gacctcttcc ccgcggcctc ccgccccgac
    20281 gtcctcgtcg ggctcgccga gccggacgac gccgccgtcc tcgacctcgg ggacgggcgg
    20341 gcgctcatcc tgacgaccga cttcttcacg ccggtcgtgg acgagccgta tgacttcggc
    20401 gccatcgccg ccgcgaacgc gatgagcgac gtctgggcga tggggggcga gcccgtcctc
    20461 gccctgaacc tcgtcgcgtt cccgccgcag ctctcggccg cgacgctcgc ggagctcctc
    20521 cgcggaggag cggacgtcgt gaggtccgcg ggagccgtga tcgacggagg gcactcgatc
    20581 caggacaggg agccgaagta cgggctctgc gtcgtcggct tcggcgagac cggtcggctc
    20641 ctgacgaagg gaggcgcacg gcccggtgat tcgctctggc tgacgaagcc gctcgggacg
    20701 gggacggtca cgaccgccct caagcgcggc ctcgccacgg aagaagaggt cgccgcggcg
    20761 gtcgcctcca tgaagactct gaaccggggc gcggcgcgcg ccgcgcgggc ggcgggagcg
    20821 aaggccgcga ccgacgtcac cggattcggc ctcctcggcc acgcgctcga gatggcggag
    20881 gcctcggacg tccgcttccg cttcgactgg gacgcgctcc ccttccttcc cggagcggcg
    20941 cgcctcgcgg ccgaaatggt cttccccggc ggcgcgttcg ggaacctcga gcacttcgga
    21001 gcgcggctcg cccgctcgcg tcccctgacc gacgccgagg cgatgctcct cgccgacccg
    21061 cagacctcgg gcggcctcct cgtggcggtg cccgaggctg gcgaagggac gttcctctcg
    21121 acgctggagc gggaaggcgg ccgcacctgg aggatcggcg acgtcgggcc ggggtccggg
    21181 atcgagatcc gctgactgcc gcgacgcctc gcgcgtcgcg gtccctcgcc tgccggagcg
    21241 ttccttgacc cccgcgctcc gcgaccggag aatctccgac gcgctcgcca cgcttcgagt
    21301 cgacgtctgc cgggcccttg ctggagctcg aacgggcgat tcgcccggcg tggccgcgga
    21361 cgtccccgga agggacgatc gaggaggaag gaaatgagaa agctcgctct cggcgccatg
    21421 gcgctgctgc tcgcgaccgt tcccgcccgg gctcaggagg actacgagag ctgcaaggac
    21481 cacccgctct tcacgcggtt cccgaactcg tacatcgtcg actgcgcgag cagccagttc
    21541 gacctgagga agttccccgt cgggcagatg aagcccggcg aggaggtgct ccggtccgtc
    21601 gaggtcgagg gaccgacctg gcgcatcaac ttccagatca aggaggggac gacccctccc
    21661 agtggcctgc agctgatgcg caacttcgag acggcggcga agaaggccgg aggcaccgtc
    21721 gagggccagt ggcccggctg gtgcaaggcg aactacgaca ccgaggggat gcccgacatg
    21781 gggaacagct gcacgagctt cggcctgacg atgaagttcg tgaaggaggc caaggagacc
    21841 tgggtcttct tccaggcgaa cgacgccgac gggagctacc tgatgaccgt atcggagcgc
    21901 gaggcgatga agcaggaggt cgcggtcaac gagatcgccg accagctgaa gaaggacggc
    21961 ttcgtctccc tctacgtcac gttcgacacg ggcaaggcga cgatctcgcc cgactcggcg
    22021 aagctcctcg acgacgccgc cgccgcgctg aaggcggcgg cggagctgaa ggtcgaggtc
    22081 ggcggccaca ccgacaacgt cgggagcgcc gacgcgaacc tgaagctctc gcaggaacgc
    22141 gcgcaggcgg tgatggcggc cctcgtgaag cgggggatcg cggccggccg gatgacggcg
    22201 aaggggtacg ggcagacggt cccggtcgcc gacaaccgga cggaggacgg gcgcgcgaag
    22261 aaccggcgcg tcgagctcgt gaagaagtag cctcgcgccg cgcgccgccg gaccggctcc
    22321 cgcgggagcc gattgcgcgt cttggggcgc gcagaccgtt tcgtcggcgc gcggagcgtt
    22381 gcagcaccct gccccagctc gagagccgct ccacggggca cggcagacca cggcgagcgc
    22441 cgctccgtcc cgaagtccac tctcctcaac accttcctgc cgcatcccgg ccgacgcccc
    22501 gggtccggaa cgcaggtctt cggggtggcg ccgttcttga actccgtctg ccgtggccct
    22561 acccgggctg cgcgggcggc ccgtgaccgg ccgtcccgga cggagcggaa tgaagaagat
    22621 cctcctcgcc ctcgtcgggc tcgcggtcgc cgcgagcctc gtcgtggccc aggtccggaa
    22681 gaaggacgcg gggccgaagc cggtcgaggc ccttcgcgag acctaccgga cgaagacgaa
    22741 accgtccgtc gaccacgccc tcttcgccca gctgaagggg ccgttccgca ggccccagga
    22801 cgtgacggcg gcctgcatct cctgccacaa cggacggcac actgaggtga tggcctcctc
    22861 gcactggagc tgggagcggg tggagtacgt cgccggcaag ggaatccgcg cggtcgggaa
    22921 gaagaacgtc ctgaacaact tctgcatcgg cgtcgcgggg agccagcaga gctgcgacaa
    22981 gtgccacgcc ggctacggct gggccgacgc ctcgttcgac ttctccaacc cgatgaacgt
    23041 cgactgcctc gcctgccatg acaacggcgg gacgtacgcg aagaagaccg gaggcgcggg
    23101 cctccccgag ccgggcctcg acctcgccgc cgtcgcccgg aaggtggggc gcccgcagcg
    23161 cgagaactgc ggaatgtgcc acttcttcgg cggcgggggg aacaacgtca agcacggcga
    23221 cctcgacgtg gcgcttctcg acacgacccg cgaggtcgac gtccacatgg ggaccgacgg
    23281 cgcggacatg gcctgcgtcg actgtcaccg ggccgagaag caccagatgc tcgggaaggc
    23341 ctactcgctc tcctcgatga accggaaccg tgtggcgtgc gagagctgcc acggcgccgt
    23401 cccgcacgag gacgacctcc tgaacaaaca cggctacaag gtcgcctgcc agacctgcca
    23461 catccccgag tacgcgaagg tgaacgcgac gaagctccgg tgggactggt cgacggccgg
    23521 gaagctgagg gacgggaagc cctacgagga ggaggacggg gacgggaacc acgcctatct
    23581 ctcgatcaag gggaccttca cctggggaaa ggacgtcgtc cccgagtacg cctggttcga
    23641 cgggacggcc tctcactacc tcctcggaga gacgttcgac ccctcgcgcc cgctcgtcct
    23701 gaacccgctc ggcggcgagt acgacgtgcc ggaggcgaag gtcgcccccg tcaaggtcca
    23761 ccgcgcccgg cagatctacg acacgaagca gcgcgtcctg gtccagccga agctcttctc
    23821 cgccgctccg ggcgacggcg gctactgggg cgacttcgac tggaacgccg cggcggccgc
    23881 cgggatgaag gaggtcggcc tcccctacag cggctcgtac ggcttcgccg agacggagat
    23941 gaactggccc gtgaaccaca tggtcgcccc gaaggagaag gcggtctcct gcgaggagtg
    24001 ccacgcgcgg gaggggggcc gcctcgcgaa ggtcggcggc ttctacctgc cggggcggga
    24061 ccggagccgg ttcctcgacg ggctcgggac gacgctcctc ctcgcgacgc tcggcgccgt
    24121 cgtcgtccac ggcggggcgc ggatctggtt ccggcggcgg cggcacgcgg gagggaagtg
    24181 agatgaagcg cgagtacgtc tacccggcct tcggccgctt ctggcactgg acgcaggcct
    24241 tcctgatcct cttcctcgcc gtcaccggct tcgagatcca cggctcgctc cgcttcttcg
    24301 gctacgagca ggccgtgcgg ttccacaacg gcgccgcctt ctcgttcctc gtcctgatcg
    24361 ccttcgccgt cttctggcac ttcacgaccg acgagtggcg gcagtacgtc ccgacgtgga
    24421 agaacgtccg cgcgcaggcc gagtactacc tcttcggcat cttccaggac gccccccacc
    24481 cgacgaggaa gacgaagctg aagaagctga acccgctcca gaagatcgtc tacgcctccc
    24541 tgaagctctt cgtcctgccg ctcgtctggg tctcgggcct cctctacatg ttctaccgct
    24601 acccgcagaa gcacgaggtc ctcgccctcg acgtcacgac gctcgagccg atcgccctcg
    24661 cccacaccgc cggggccttc gccctcctcg tcttcctcgt cgcccacctc tacctgacga
    24721 cgacggggca caccgtcacg tcgaacctga aggcgatggt gaccgggtgg gaggacgtcg
    24781 aggaggagcc cgcgcccggc gccgggccgt cccacgccgc cgcgacgccg gacgcccgct
    24841 gaggagatcg ccgtgaacga ccgaccgatg aacccctacc tcgccggagt cctcctcggg
    24901 ctcgtcctcc tcctgacggt cttcgtcacg gggcgcggcc tcggcgcgag cggcgcgacg
    24961 aagagcgtcg tcgtggccgc cgtcggcgcc gccgcgccgg agcacgcggc cgcgaacccc
    25021 ttctacgcga agctcgtcgg ccccggccac gagaaccccc tcaaggcgtg gctcgtcttc
    25081 gaggtcgcgg gcgtcctcct cggagcgttc gtctcgggcc tcggcgcggg ccggctgaag
    25141 gtcgggacgg agcgtggacc gcgcgtctcg gtgagggggc gccttctctt cgccgccgcg
    25201 gggggcgcgc tcttcggcgc gggcgcgcag ctcgcgcgcg gctgcacgag cggcgcggcg
    25261 ctgagcggga tggccgtcct ctccgtcgcc ggcttcgtga cgatgctcgc gatcttcggg
    25321 accggcttcg ccttcgcgtg gttcacgcgc gggctctggc tccccgggaa ggcgaagagg
    25381 taggcgatgg gaccgttcgt ccccgacctg atcaccgacc agctcaacct cgtcgtcgcg
    25441 ctcgccgtcg gcttcctctt cggcttcgtc ctcgagcagg ccggcttctc ctcctcgcgc
    25501 cgcctcgtgg gcgtcttcta cggctacgac ctgacggtcc tgcgcgtctt cttcaccgcc
    25561 gccgtgacgg cggccgtcgg catcctgctg ctcgccgcgg gcggcctcct cgacctgaag
    25621 gcgatcttcg tcaaccccac ctggctgtgg cccgcgatcg ccggaggggc gatcatgggg
    25681 ctcggcttcc tcctcggcgg cttctgcccg gggacgagcg tctgcgccgc ggcgatcggg
    25741 aaggtcgacg cgatggcctt cctcggcggg agcgtcctcg gcgtcttcgc ctacgcggag
    25801 ctcttccccc gcttcgcggg cttcgccgac tcgagcgccc tcggcccggc cctcgtcccc
    25861 gacgcgctcg gaatctccgc cggcctcttc gtcctcctcc tcgccgccgc ggcgatcgcg
    25921 gccttcgcgg tcggcgggat cgtcgagcgg aaggtcgccg gggctgcggc gccctcgagc
    25981 gccttcccgc gccgcctcca cgtggcctcc gccgcccttc tcctcgccgt cgcggggctc
    26041 gcggccgccc tccccgaccg gaaggcgcgc ctcgtcgcga aggtgagcgc tccgggctac
    26101 gccccgcacc acctcgtccg cccgatgagc cccgacgagc tcgcgttccg gatcgtcgac
    26161 ggcgaccatc ggctccgcgt cgtcgacctg cgcggcgagg cggcccgggc ggcgaggccg
    26221 ctccccgggg ccgtctccgt cccggcggat gacgtcctcg gccgggagtg gcgggacgtc
    26281 ttcgcgagga ggatggtcca gaaggtcgtc gtcgccccga ccgaggagga ggcgcggacc
    26341 gcggccttcc tcctcgtgga gaccggcttc gagaacctcg cctacctcgc cggcggtttc
    26401 ccggccttcg agaggacgat cctgacgccc ggcgcgccgg ccggcgagcc gcgcttcgcc
    26461 ccggtcgtcg aacgattccg cacggaggcg cgggcggcgc tcgccgagcg cgcgaggctc
    26521 gagaaggaag ccggcacgcc cgcgccgagg aaggcgaaga aggtccaggg ggggtgctga
    26581 agaggccccc gcggctttcc gcgcggccgg agccgcgcat actcggagaa gccggcgtgg
    26641 cccgaagcga ccgtgccgtt ccgcggaggt gaccgatgga cgccgtgacg ctttcgagga
    26701 tccagttcgc cgtcagcatc gggttccact acatcttccc gccgctgacg atcgggctcg
    26761 gcgtcgtcct cgtctggctc gaggcctggt tcctcaagac gcgggacccg atctacgaga
    26821 ccgcggcccg cttctggacg aaggtcttcg ccctcaattt cgcgatcggc gtcgcgacgg
    26881 ggatcgtcat ggagttccag ttcggcacga actgggcgac ctactcgcgc ttcgtcggcg
    26941 acgtcttcgg ctcggcgctc gcggccgagg ggatcttcgc cttcttcctc gagtccggct
    27001 tcctcgccgt cctcgtcttc gggtgggacc gcgtcgggcc gaagatgcac ttcttcgcga
    27061 cgctcatggt ctcgctcggc tcgatcttct cctcgatctg gatcgtcgtc gcgaacagct
    27121 ggcagcagac gcccgcgggg caccacgtcg tcccggtcct gcgcgacggc gtcccgctcc
    27181 tccgcgacgg ccagcccgtg atgcgagccg agatcacaga cttctgggcg atggtcttca
    27241 acccctcgac cgtccaccgc ctcgtccacg tctggatcgg ggcgttcctc gtgggcgcgt
    27301 tcttcgtcat gtcgatcgcg gcctggtacc tcctgaaggg gaagcacggc gagttcgcgc
    27361 gccgctcctt cgacggcggc ctcgtcctcg ccaccgtctc ggcgctcgcg atgctcgtct
    27421 ccgggcacgg gcaggcgggg agcgtctacc gccatcagcc cgcgaagctc gccgcgttcg
    27481 aggggcacta cgtcaccggg ccggccgacc tgaacctctt cgggttcccg gacgacgcga
    27541 cgcagtcggt gaagctcggc gtcgccgttc ccggcgggct cggcctcctc ctgcacggcg
    27601 atccggcgat gccggtggtc ggcctggacc gattccgccc cgaggaccgc ccccccgtcg
    27661 gcctcagctt ccagagctac cacctgatgg tcgcgctcgg gatggccttc atcggcctga
    27721 cgctgctcgc ctcgttcctg aggtggcgcg ggacgctgta cgagacgcgc tggctcctct
    27781 ggctcttcgt ccccgccgtc gtcctgccga tcgctgccaa cgagctcggc tgggtggcgg
    27841 cggaggtggg acgccagccc tggatcgtcc acccgccggt gacgtggacc gccgacggga
    27901 cggacgtcgt cgtcgggccc gaggggcgcg tcgtctacga cgagcgcctg ggcctccgga
    27961 cgctcgaggc ggcgagcccg aacgtttcgg cgggccaggt gctcggctcg ctcctcggtt
    28021 tcggcttcat ctacctcgcg ctcggggccg tctgggtctt cgtcctcgac cggaagatca
    28081 agcacgggcc ggatggggcc tctcctccgc ccggcgggag ccgcgacggg gccctcgaag
    28141 ccgccgcgct tcgggccgtt cacgagggac ggatgacgga cggcggcgag gcgcggggct
    28201 gagggggcgg cgatgtcttt cgaagcgctc gccctcacct ggttcgtcct ggagggggtc
    28261 ctcctgaccg gctacgcgat cctcgacggc ttcgacctcg gcgtcggaat cctccacccg
    28321 ttcgtcccgc ggaccgacct cgagcgacgg atctcgatga actccatcgg cccgctctgg
    28381 gacggcaacg aggtctggct cgtcacgttc ggcggcgccc tcttcgccgc cttcccggag
    28441 gcctacgcga cggtcttctc gggcttctac acggccttca tgctcctcct cgtcgcgctg
    28501 atctctcggg cggtctcgat ggagttccgc tcgaagatgt cctcccccgc ctggcggagg
    28561 ttctgggact gggccttctt cggcggctcc ttctccgcga cgctcctctt cggcgtggcg
    28621 gtcggcaacg cgatgatcgg cgtgccgatc gacccgaacg gcaacgtcac cggctccgtg
    28681 ctcggccagc tcggtcccta cccgcttctc gtcggggcga tgacggtcgc actcttcgcg
    28741 atgcacggtg ggctcttcct ctacctgaag accgaggggg atttccagca gcgcctccgc
    28801 gagtggatgt ggcgctcctg ggggctcttc atggtcctct tcatgctgac gaccgtcgcg
    28861 acggtcgcct tcatcccgcg ggcgacggag aacttccgga gcttcccgtg ggccctcgtc
    28921 gtcgtcgtcg ggacgatcct cggcgtcgcg aacatcccgc gctgcctcta ccgcgaccgg
    28981 cccggcctcg ccttcctctc ctcggcgggg gtcatcgcgg ggctcgtcgc gctcttcggc
    29041 ctcgcgctct acccgaatct cgtgacggcg gcgggggacc cgtcgcgctc cgtcacgatc
    29101 ttcaacgcgg cgtcgagccc gaagacgctc ggcatcatgc tcgtcatcgc cgcggtcggg
    29161 atgccgttcg tcctcaccta ctccggcgtc atctactgga ccttccgcgg gaaggtgaag
    29221 ctgggcgagc acagctactg aggcgaccag agccgtttcg ccgttcgccg aaacgagcat
    29281 atgatctctt cggcccctcg gggccagaaa ggagaagcga tgagcgaacc cgtgggatgc
    29341 ggtcgcgtgt cgggcgccgt ctccggcgaa ggacccccgc ccgcacccgg ccccgacacc
    29401 gaacggaaga aggaggtgct cgtgacggca cgcgtgggcc agaaggcccc ggaattcgct
    29461 gccccgtcgt actaccgcgg cggcttcacc cccgtgaagc tcgccgactt cgccggcaag
    29521 tgggtcctcc tctgcttcta cccgggggac ttcaccttcg tctgagcgac ggaagtgtcg
    29581 gcggtcgccg acaattacgc acggctccag gcgctggggg tcgaggtcct ctcggtcagc
    29641 gtcgacagtc atttcgttca caagatgtgg aacgaccacg agctcgtgaa gatggtggac
    29701 ggaggagtcc cgttccacat ggcctccgac caggcgggca acatcggccg cgcctacggc
    29761 gtctgggacg agtcccaggg gatcgagctc aggggccgct tcctgatcga cccggacggc
    29821 atcgtccagg cgatggaggt gatgacgcct cccgtcggcc gcaagctcgc cgagacgatc
    29881 cggcaggtgc aggcgttcca ggtcgtgcgc gcctcgaagg ggacggaggc gacgcccgcg
    29941 ggctggatgc cgggagaccc ggtgctcagg cccggacccg acctcgtcgg gaacgtctgg
    30001 aaggtctgga agccctcgat ggagaagtga cccgggctcg ctcggggcgc ggggaacggg
    30061 gatgacgaaa cgcgctgctt cggacctccc tgtctccccc gcctcgagcg aggcgatcct
    30121 cgagagcatc tcggacggcg tcttcaccgt cgaccccgag tggcggatca cgtcattcaa
    30181 ccgcgccgcc gagcggatca ccggcgtccc gcgccgggag gcgatcggga ggcgctgcgc
    30241 ggaggtcttc cggtcgagca cctgcggccc ctcctgcgcg ctccagaaga cgctccggac
    30301 ggggaagccc gtcatcggga agtcgaccta catcgtcgtg gcggacggga cgcgcgtccc
    30361 ggtcagcctc tcgacggcgg tcctccggga cgcgaaggga agagtgacgg gcggggcgga
    30421 gatcttccgg gacctctccg aggtcgaggc gctccgggag gagctcgagg gacgctcacg
    30481 agtcggcgac ctctcgaccc gcagcccgct gatgcagcgc gtcctcgaag cgctcccggc
    30541 gatcgccgcg agcccgagca cggtcctcct tctcggggag accgggacgg gcaaggagct
    30601 cgtcgcgcgg acgatccacg cgctcagccc gcgggcgaag gggccgttcg tcgccgtgaa
    30661 ctgcggggcc cttcccgaca cgctcctcga gtcggagctc ttcggccaca cggcgggcgc
    30721 cttcacgggc gccgtgaagg aacgggccgg gcgcttcgcg ctcgcccagg gggggacgct
    30781 cttcctcgac gagatcggcg aggtgagccc cgcgctccag gtccgcctcc ttcgcgtcct
    30841 ccaggagagg acctacgagc cgctcggctc gagccgctcc gcgaggaccg acgcccgggt
    30901 catcgtcgcg acgaatcgcg acctcgccgc gcgggtccgc gaagggcact tccgggagga
    30961 cctgtactac cgcgtgaacg tcgtgcggat cgagctgccg cccctccgga gccgcaagga
    31021 ggacgtcccg ctcctcgtcg aggagctcgt cgcccggttc aaccgtctcc aggggcgcgc
    31081 ggtgaagggg atcaccgccg aggcgctctc cctcctgatg gcgcacgact ggccgggaaa
    31141 cgtccgcgag ctcgcgaaca tcgtcgagcg ggccttcgtc gtctgcggcg agagacggat
    31201 cgacatcggg gaccttcccg acgagctggc gggccgcctt cggcccggcg cgccggagct
    31261 cgacgtccgt tccgcgcacg ccgtcctgga cgccgaggcg atccgctcgg cgctggagcg
    31321 tcacgggttc aaccgcctcg aggcggcgaa ggagctcggc atccacaaga cgacgctctt
    31381 ccggcggatc cgcaagctcg gcatggagct cccggaacag gacggacgct ccgcgcggtc
    31441 cgagacggag tagcgcttac gctaccgtag cccccgaaca aggtagccac ggcgctactc
    31501 gattacgcgg ggacgaacct cacctcccgc acgcatcctc ttcgcccgga gtgagttcgg
    31561 cgacactgcc ggtgctcggt ccggcctggc gcggcgtttg cgcggttccg ggcgtggacc
    31621 gagaacgcac ccccctccgg acgaaagcgg ccttcccctg ctgggacggc cggatcgcac
    31681 ccgttttcga cacggcgcgg gaccttcatg tcgtctcgac ggagtccgga agggtcgtct
    31741 cgacgtcgga ggagccgctc ccctcgggac cgccaggccg gaaagcagcc cgcctgtccg
    31801 agctgggcgt cgagaccctc gtctgcgggg cgatctcgcg gccgatgcag gacgcggtcg
    31861 cctccttcgg catccgggtc gtctcgttcg tctccggaga gagggacgag gtcgtccggg
    31921 cgtggctcgg aggggagctg gcccggccgg ctttcacgat gcccggctgc ggcggacggc
    31981 agcgcgcgcg attcagagga cgtttcggcg gaggcgacac gggatcgtgc ctctgcccgg
    32041 cttgtgggca ctcggagcct cacgagcgcg gaattccctg cgttcagagg ccgtgcccga
    32101 aatgcggcac cgcgatgagg cgggcctgag gaaaggagaa cgagtcatgc cgagacgaga
    32161 tggaacggga ccgatgggac agggatcgat gacgggtcgc ggagcgggcc cttgctccgg
    32221 agacgtggcc cagggagccc ccggacaggg gcgtggccgg ggattcggac tcggcttcgg
    32281 gagagggttc ggcaggggcg gaggcgcggg cttcggccgg ggctggcagt ggtggcggcg
    32341 cggagccgtc gccggtgccg ccgtcccgcc ggccgcggtc tccgacgccg cccgcgaaga
    32401 agaggccctc cggatgcgca tcgacgcgct gaaggaggag ctggcgtgcc tggagaaggg
    32461 cgtccgcgcg atgaagggcg acgccgacaa gggctgagac atggcgaaga tcggaatcat
    32521 catctgtgcg cgctacaagg actgcggcgg cggcaagtgc ctgcggtcga tgcgcgagcg
    32581 ccgcggggcc ttcgccgcgt acccggccga cgagccgctc gagatcgtcg gctacgggag
    32641 ctgcggcggc tgcccgggcg ggaacgtgga gtacgtcccg gccgagatga agaagaacgg
    32701 ggccgaggcg atccatctcg ccaccggcct cgtcgtcggg tacccgccgt gcccctacat
    32761 ccgcgagttc aagcagtaca tcgagaccgc ctacggcctg ccggtcgtcg tcgggacgca
    32821 cccggtcccg gagaaatacc ggaaggtcca cgagcagctc cccttctggg acgacagcgg
    32881 gatggccgag ctcgccgggc cgctcctgaa cgaggacccc gtcatccaga aggcctacga
    32941 ctgaacgaaa gggagacgat gaagatcgcg ttttcgacct cgggagagag cctcgaagct
    33001 ccgctggaca gccggttcgg ccgggctccg tacttcctca tctacgacct cgactcgagg
    33061 caggcccggg tcgtcgacaa cggcgcgagc cgggcttcga gccagggggc cggcatccag
    33121 gccgcggaca gcgtcgtccg ggccggggcc ggggccgtcg tcacggccca ctgcggcccg
    33181 aaggccttcc aggtcctcca cgcggccggc atcaaggtct tctcctgcca cgccgccacc
    33241 gtcggggaag cgctggacat gtaccgctcg ggagccctgg tcgagcagac cggcgcggac
    33301 gtggcgggcc accacgcatg aaggtcgcga tcgcctcggg caaggggggg accggcaaga
    33361 cgaccgtcgc cgtgaacctg gcgcggagct ggggcggccc cgtgcagctg ctcgactgcg
    33421 acgtcgagga gccgaacgcc cacctcttcc tcggcggcga gaagggggag gaggtccccg
    33481 tcttcgtcaa ggtcccgtcc gtcgacgagg ctctctgcga cgggtgcggc gagtgcgggc
    33541 gcttctgcga gttccacgcg atcgtcgtcg tcggcaggtc ccccatcgtc tttcccggga
    33601 tgtgccacgc ctgcgggggc tgcgcgaagg tctgcccgcg aggcgccatc cgcgaggtgg
    33661 acgagaggat cggcgtcgtc gagacgcggc gctcggggtc cgtctcgctg accctcgggc
    33721 gtatggacgt cgggatgagc atggcgccgc ccctcgttca cgccgtccgc gcgcggatcc
    33781 agggcggctc cccggcgatc ctcgacgccc cggcgggcac ctcgtgctcg gtcgtcgcga
    33841 cgctccgcga gacggacttc gtcgtcctcg tcaccgagcc cacccccttc gggctacacg
    33901 acctgacgct cgccgtcgac ctcgtgaggg agctgcgcct cccgttcggc gtcgtcgtga
    33961 accgcgtggg agccggcgac gaccgggtcc acgcctggtg ccggaaggag ggggtcgaga
    34021 tcctcctcga gatcccggac gaccggcgga tcgccgaggg ctacgcccgc ggacggctgc
    34081 tcgtcgaggc gctgcccgag tacggggacc tcttccgctc gctcgccggg acgatcgccg
    34141 cgcgggtcgg ggtggcggcg tgagcgcggc ggagcgtgcc ttccggcacg tctacggacc
    34201 cgtcccgtcg cgccggctgg ggcggtccct cggcgtcgac ctcgccccct tcaagacctg
    34261 cgcgtacgac tgcgtctact gccagctcgg gcggacgacg gtgaagacgg cggagaggaa
    34321 ggagtacgtc cccgtagagg aggtcctctc cgagctccgc gagtggctgc ggagagggga
    34381 agcgtgcgac tacatcagcc tcgccggctc gggcgagccg acgctccact ccggcctcgg
    34441 cacgatcgtg agggcgatca aggggatgac gaaggtcccc gtcgcggtcc tgacgaacgg
    34501 ctccctcctc ttcatgggcg aggtgcagga cgccctgatg gaagcggacc tcgtgatccc
    34561 ctcgctcgac gcgggggacg cccacctctt ccagtgggtc aaccggcccg tcgaggagat
    34621 ctcgttcgac cggatggtcg aaggcctcga gtcgttcacg aagcggttcc ggggagaggt
    34681 ctggctggag gtcctcctcc tcgaaggcgt cacggcctac cccacccagg cggagaagat
    34741 cgccgcgctc gtccgccgca tcggtccggc gcgggtccag ctcaccacgg cttctcgccc
    34801 gccggccgag ctcttcgcca ggcgggtgtc cgagggccgg ctgctcgcgc tcgcgagcct
    34861 cttcccgggc cgggtggacg tcatcgcgac ccgggaagag ccggatgcgt cggtcggatc
    34921 gagcccgggt acgggcgcgc ccgcgatcct gacgctcctc gagagacggc cctgcacggc
    34981 cacggacgtg gccgtggggc tcggtatcca cccgaacgag gcgctcaagg tcctcgaccg
    35041 cctcgtcgtc gccgggacgg tgaagaccgt cgcctccgcc gacaggacct tctacatcgt
    35101 cgcggagagg ggcgtgccgg cgggatcgcc cgaagggggg cgtcagagat gagggagatc
    35161 gtcgtcgtca gcggtaaggg agggacgggc aagacgagcc tgacggcctc gttcgcggtc
    35221 ctcgccgacc gtcccgtcgt cgccgactgc gacgtggagg cggccgacct gcacctcgtc
    35281 ctctcgccga cggtcgtcga acggcacgag ttccggggcg gccgggaggc ggtcatccgg
    35341 gaggaggact gcctgggctg cgggatctgc ctcgaggagt gccggttcga cgccgtgagg
    35401 aggatcgaga agggcggagg gcgcgccacg tacgccgtcg acccgacggc ctgcgaggga
    35461 tgcggcgtct gcgtccgctt ctgcccggag cacgcgatcg acttccccga gcggcgttcc
    35521 ggcgagtgga tggtgtcggc gacccgttgc ggcccgatgg tccacgcgcg gctcgcgacc
    35581 gcggccggca attccgggaa gctcgtctcg gaggtgcggc aggaggcgcg gcggatcgcc
    35641 gatgcggagg gccgctccct cgtcctcgtc gacggtcccc cgggggtcgg ctgtcccgtc
    35701 atcgcgtcgg tcaccggcgc gtccctcgtc ctcgccgtca cggaaccgac ggtctccggg
    35761 gagcacgacc tcgagagggt cctctccctg gcgcggcact tcggagtccg ggcggccgtc
    35821 tgcgtcaaca agtgggacct cgaccccggt atggcggacc gcatcgagca gctcgcgtcg
    35881 aaagccggcg cgcgcctcgc cggacgcgtc cggtacgaca ggggtgtcac cgcggcgcag
    35941 atgctccaac gggccgtcgt cgagatcgat accccgagcg cggcggacgt gcggcgggtc
    36001 tgggagagcc tcgccggatg aatctcggag agccggggga ggctgcgccg tgccggcctc
    36061 gcgcctcccc tcctcccgcg gattcgcgtc gccggaggcg aaccccggcc gcagcggtgg
    36121 acgaggccga agtcctgaca gcgggagcgg acgggccgaa actcccgagt cagcgaacgc
    36181 cgatccgcag ctcccgcatc cgcctccaga gcttcgtcct gctcatgccg agatcggccg
    36241 ctgccttcga gcggttccag aggttccgct cgagggccgc gcggatgggc ccttcttcgg
    36301 ggtcgtcccg ctcggacgcc gggtccctcg cctgccgcgg cgtcgcgagg gatcgttcac
    36361 ggcttcggtc gccgccggac gcgaacggct ccggcgcgcg gatctccggc ggcaggtcct
    36421 cgagctgcac cgtctggccg cgacccagcg ccacggcgta ctcgagtgcg ttctccagct
    36481 cccggacgtt ccccggccac tcgtagcgct cgagggcggc gagcgtgtcg ggagagagct
    36541 gcacggcgcg gccggcacga cctccgattc gggcgaggag gtaccgggcg atcagctcca
    36601 cgtcctccgt ccgctcccga agcggcggga tgtggatcgg gacgacgcgc aggcggtagt
    36661 agagatcctc ccgtagacgt cccgtccgca cggcctcctt cggatcgcgg ttcgtcgcgg
    36721 cgacgatgcg cgcgtcgagc ggccgcggcg tgctctcgcc gacccgctcg aagacccgtt
    36781 cctgaaggac ccgaaggagc ttcacctgga ccgggagcgg gatctccccc gcctcgtcga
    36841 ggaagagcgt gccgccgcgc gcgaggtcga gccttccggt ccggtcgcgg accgcgccgg
    36901 tgaaggctcc ccggacgtgt ccgaagagct cgctctcgag caggtcggcc ggcagcgccg
    36961 cgcagctcac cgcgacgaac ggacccgagc tgcgcggcga gtgggcgtgg agggcccgcg
    37021 cgaggacctc ctttccggtg ccgctctccc ccgtgatcag gacgttcgcc tcgctcgagt
    37081 ggagggactc gacgaggtgg acgatccgga gcatctgcgg ggagcgggcg acgagcccgt
    37141 gcgacacggc cgcgctctgg aggatcgtgt cgtcgtcctc ggcgggacgg atcacggcga
    37201 ggtagcgcgc ccgcgggtcg caggcgtcga cgacgcccgg ggcgagcggc gtcgtcgtga
    37261 tcgacacgag acgcgccgag tcgcgcccgc accggaggac tccgcggcgc ccctcctcgc
    37321 gccggccgag ccggagcgat tcgcggagcg tctcctcggg gccgaagagc cgcggtccga
    37381 agagcgactc gaccggctgg ccgatcacct cggcggcgac gccctccccg gccatccgct
    37441 cgagcgaacg gctcgcacga atgatgacga aaccctcgtc caggacgacg agggcccggc
    37501 cgagcgattc gaagaccgcc tcgaggttcg accagtcggg ggcgcgctga gtctctcgat
    37561 ccatccggtg cgggggcgga gcacccccgg gcgcgattct gcccgaggtc agccccggct
    37621 cgggaggccc gcgccgtgtc cgtggtcgtg cccgtgcccg gcgcacgccc cggccgggtc
    37681 catctcctcg agttcgttcc ggaggaaggc gtcgaggcag acggaggccg tcggcgccgt
    37741 cgcgcggaag acgcgaatcc ccgccgactg gagcttcatg atcgctcccg cgccgatgcc
    37801 tccgacgagg aaggcgtcga tccgctcccc ggcgagcgcg tcgagcggac ggcaggcgcc
    37861 gtgctcatgg acggctcggg cgttcgagag ggtgcgcgtc gcgcgggtct ccgagtcgac
    37921 gaggaggtag agcggcgcgg aaccgaagtg tccggagacc gggctctcga ggccgcggtc
    37981 ggcggtgacg gggatgcaga ggttcatcgg ggctccttcc ggccgccgcg atccgcgggg
    38041 cgcccgatcg ggcccccgcg tccccgccgc gggccacccg acagtttcgg gcatatgccc
    38101 gtatgggtca aggacgatcc gcgctcctag cggcgacctc ccgccccgtt cgcgcgagcc
    38161 ccgcgacgac ccgttcgagg tcggccgccg tcgtccgcgg gttcatctgc gtcgcgcgaa
    38221 ggacccgccg gccaaccagg agcgtcgtcg tgatccatcc ctccccggag cggttgaacg
    38281 cctcgcggag gcggaggttg atcccgtcga ggcggccggc gtcgagcttc tcttcgccga
    38341 cgtaccggaa gcagaggatg ttcgactccg gctcgtggag cgtctcgaaa tcgcgccggg
    38401 ccgcgagcgc cgcgtggagc gccctcgccg tcgtgcagag gccgtcgtag agggcgccga
    38461 ggccttccgc gccgtagcgc tggaaggcga cccacacctt cagcgcgtcg agccgccgcg
    38521 agcactggaa gctccgcttc ccctggtcgg ggctccgctc tccctccgcg ccgtggaaca
    38581 ggtacggggc gcgctgggcg aacgcggcgt cgaggtcgcg ctcgtcccgc acgaggagga
    38641 cgcccgccga gagaggcatc agcatcatct tgtgcgggtc ccaggcgagc gagcgagcgc
    38701 gggcgatccc gtcgacccgc ccgcggtgcg ccgaggagag gagggcggag gcgccgtggg
    38761 ctccgtcgac gtggagccag acgccgcgcg cctcgcagag gcggccgatc gcgacgaggt
    38821 cgtcgaacga gcccgtcgcc gtggagccgg ccgtcgcgac gacggccatc acccgccgcc
    38881 cgcgggccgc ctgccgatcg agctccgcct cgagcgcggc gacgtccatc cggaaggcgc
    38941 gcgaggggac cgccacggcg gcgtcggtcc cgagcccgag cgcgccgacg gcccgggaga
    39001 cggcgtagtg cgcgtgctcc ccgtgcagga cgaccggcgg ctccgccccg actcccttcg
    39061 tccaggcgtc ggggagcgcc gcagcgcgcg ccgcgaggag cgccgtgaac gtcgcctcgg
    39121 tcccgcccga ggtgaacgtc ccgccggctg cgggaccgaa tcccgcgagg tcgcacatcc
    39181 agcggacgac ccggccctcg atcgccgtcc cggcgggcga catctcgaag acggcgagag
    39241 actggttcag cgcggcggcc agcggctcgg tccagatcgc ggcggggagg ggcgccgaga
    39301 cctggtgccc catcgagcgc gggtggcaga ggcggttcga ggcggggacg acctcgcgtc
    39361 ggagacgctc ggcgacctcc gcgagaggcc ttccccgccg gggcaggggc tcgtcgaaga
    39421 ggcgcgcgag ctcccgcggg gtgagcggtg tcgagacggg gccgtcgccg gagcgcgtct
    39481 cggccaggta ctcggccgcg atcgacacga gagtctctgc cgcggaagga acggcgtcgg
    39541 cctcgagctc gtggaggacg tcggggagcg cgtcgggcat cgatcgaagg tagcacgcgg
    39601 cccgcggcga tccgttgcca cgccccggac gcggccggag aatcgaccgt cgctcccgcg
    39661 aggaggtccg catgccgcgt cgctccgcct cactccggct cgccggtctc gccattctcg
    39721 tcaccgtcgc cgcgctcgcg cgaaccgccc cggcgcacga ggtccgcgtg aggagaccgc
    39781 tccgggtccc ggacatcccg ggctacgtca ctctccgctg cgacttccac acgcacacgg
    39841 tcttctccga cggctccgtc tggcccgaca tccggtccga ggaggcgtgg cgggaggggt
    39901 acgacgcgat cgccatcacc gaccacatcg agtaccagcc gcacaaggac gacctcccga
    39961 cgaaccacga ccgcccgttc gagatcgccg cgccgctcgg ggagaagctc ggggtcctcg
    40021 tcctccgcgg ctccgaggtg acgcggaaga tgccgcccgg ccacttcaac gcgatcttcc
    40081 tgaagatgtc gaagccgctc gagacccccg agtggaggga cgccctgaaa gcggcgcggg
    40141 cgcagggcgc cttcctcttc tggaaccacc ccggctggcg cggccatcag aaggacggcg
    40201 tcgcgcgctg gtaccccgag cacgacgaga tcctcgcggc cggcctgatg gacggcatcg
    40261 aggtggcgaa cgaccgcgag tactaccccg aggcgcaccg ctgggcgctc gagaaggacc
    40321 tcgcgatcct cgcgaattcc gacatccacg acccttcgaa cctcgcctgg cacgtccacg
    40381 agggggaccg ccggcccgtc acgctcgtct tcgcgaagga gcggagcgag gaggggatcc
    40441 gcgaggcgct cttcgcgcgg aggacggccg cgtgggccga cgggaagctc gcggggcggg
    40501 agcgcgagct ccgcccgctc gtcgccgccg ccgtgacgat cgagaacccg aagctcgccg
    40561 tccgcgccgg ggacgagggg tacgtgcggc tccggaacga ctccgacctc cgcctcgtcc
    40621 tcgaggggga gggagcggcg ggcgaggtcg tcgtcccggg gacgctcgtc gtcgccccga
    40681 acgggacgag cctcctcccg gtgagggtcc cgaagaagac ggccccggga ccgcggcgcc
    40741 tcgagctcgt ctacgccgtc tcgaacgtca ggccgacgcc cgacgcgggg ctcccattcc
    40801 ccgtcgcaat cgaggtcgag gtcctcccgc ccgctccaaa gtagtcccgg ctctcagccc
    40861 gggaacgtcc gcgcctcgtc gaaggcggcc gtgacctccg ccggcggcgc gggcgtcagg
    40921 cggctgacga cggcgatcgc gagcgaggag aggaggaacg cggggaggag ctcgtagacg
    40981 gcgaacgctc ctccgagcgg ggcgacgagg ttcttccagg cgacgacggc gatccctccc
    41041 gtgagcatcc ccgcgacggc ccccgcgagc gtcgtccgcc tccagaagag cgagaagagg
    41101 acgaggggac cgaacgcggc gccgaggccg gcccaggcgt aggagacgac gcggaagatc
    41161 gagtcgttcc ccgagagggc gacgaggatc ccgaacgcgg tgaccgcgac ctgcgtgccg
    41221 cgcgcgagcc agaggaccga ccggtccggc agcggctcgg cccggaagtt ctgcacgagg
    41281 tcgttcgcga gcgaggagga ggcgatgagc atgtaggagt cggccgagct gatcgaggcg
    41341 gcgacgatgc cggagaggac cagccccgcg aggagcggcg ggaagaagtc gaccgcgagg
    41401 tggatgaaga ccgtctccgc cgcgctcgcg gtcaggagcc gatcgggcat cagggcacgg
    41461 ccgatcaggc cgatggcaac ggccgagaag agcgagacga cgcaccaggt gaccgcgatc
    41521 acccgggccc tggagaccat cgcgggcttc tcgatcgcca tgaagcggag gaggacctgc
    41581 ggcatcccga agtagccgag gccccaggcg aggcaggaga ggatcgcgag gaagccgtag
    41641 ccgacgccgg gaccgaacgc ggggttcccg tcgacgagcg cctgggcgtt cccggcggcg
    41701 gccgtcaccg cggccccggc ggcgtcgagg ggcgtcgcga cgccgaagag gtcggtgaag
    41761 cgcgggatcg ccccgagctc ggcgaggagc gccccgacgc cgccggcctt cgccgtcccg
    41821 accgaaagga cgaggacgag cgcgcagaac atgagcgtcc cctggatgaa gtcggacatc
    41881 gtctcggcga ggaagccgcc gacgaacgtg taggcgagga cgaagagcgc gccgaagacg
    41941 accatcctcg cgtagaggcc ctccatcccg aagacgtagc caaagagctt gccgaacgtg
    42001 acgaactggg cgccgacgta gatcgagaag aagaagagga gcatccccgc ggcgatcgtc
    42061 gagaggaggc gtcggccgtc ccggaagcgg gcgctgaaga aggccggaag cgtgaaggcg
    42121 ttccccgcga cgatcgagtg ggcccgcagg cgccgcgcga cgaggctcca gcaggcgaac
    42181 gtgccgaggg cgaggccgat cgcggtccag gccgcctcgc ccgcaccgga gaagtaggcg
    42241 acgcccggga gccccatgag gagccagccg ctcatgtccg aggcctccgc gctcatcgcg
    42301 gcgacccacg gcccgaggcc gcgctccccg aggaagtagg cctcggcgct ccgggcgctg
    42361 cgcctcgagt accagaggcc gacgccgatc gtgagcgcca ggtaggcgcc catcgcggtg
    42421 aggaggagga ccttgtccga gggcattccc gattatcggg tctgaccgcg gcggggcggc
    42481 cgccgcgggc cgcagacgcg gaacgggggg agaaggcccc cctctccccc cgtccgtccc
    42541 agcgctgcga acggcgcggc gccccttcgc gactaccggt tcgggcagcc ggcgcccgtg
    42601 ccgtcgccgg ggccgaagcc cgcgcccgga ccggtgccgg cgcccggccc cgtcccgtcg
    42661 cacgtcccgg tgccgttccc ggcgcccatg ctgcggccac ggccacgccc catgcggccc
    42721 ggacccgtcc ccgtccccgg caggagcttg ccggtctcgt cgacggcacc ccgctcgcgc
    42781 ggcgcggcga ggatccgcgc gacctcgtcg gccggaaggt actgcgcggc gtagggagcc
    42841 ccgagcgcgg agaggcggcc ggcgaaggcg cggaggtggt tccgggagcc cttcgcgagg
    42901 ttctgcatca cggcgtcgac gtcgcggttg tcggcggcgg cgagcgcctt cgcgacgtcg
    42961 gcgaggtcga ggtcctcgat cgtggcgccg accttcagcg cctcgacgag ggagaccttc
    43021 cccttcgcga cgaggtccgt gtagagggcg gcgagacgcg agtccttgaa ctcgccgggg
    43081 gccgtcgcgg cagccgggtc ggccagctcg tagcggtcga ggaggagctt cacgaggtcc
    43141 atgtgccgct gctccgcggc ggcgatgttc gcgaatgcgc ggtcgccgtg ggcggcgaag
    43201 agcgcgcggt agacgtcgcg ggcgagcttc tcctcttcgc ggaggaacgc gatctcggcg
    43261 gcctcggcgg agccgagcgg ctccttcgga agcgaggcga agtacgtcgc gaaggggctc
    43321 gccgcgggcg cggcgggagc ggcgggcacg ccggcgcccg ggccgttccc ctggcctccg
    43381 cgggggccgg gctgggcgag gagcggcgtc gcgaggagga gggcggcgag gagggcggtc
    43441 ttcttcatgt tcggctcctt gatcggtgcg ggcggccacg gtctcgggcc gcctcgtcgc
    43501 ccttcatcca cgcccggtgc cagccgtcgc acgcagggcc tgagcgcagc gaagtggttc
    43561 aggcggagcg actttcccgc cgccgccggc catctcgccg aggtgaggga ctgcaaagtc
    43621 cgcagtcccg ctgcacgggt gctgcagggg cgcccgtcgt tcccggagaa gggctcagcc
    43681 gattccccac tgcttgaggc ggtagtagag cgtccggacc gagatgccga ggagcttcgc
    43741 ggcctcctcg cgctttcctc cggtccgtcg gagcgcggcg aggagcgcct cccgttcgcg
    43801 gtgggcgcgg tcgagcggcg cgaacgccgg cgaggccggg gcgaggccga ggtcggcgcc
    43861 gcgaatcgcc tcccctcccc ggacgacgac ggcccgctcc agcacgttcc gcagctcccg
    43921 gacgttcccc ggccaggcgt gcgccgagag ggccgcgagg gcgtcgtccg tgagcgacgg
    43981 gacggggacg ccgtgccgcg cggcgagctt ctccgcgaag tgccgggcca ggagcggtac
    44041 gtcccccggc cggtctcgaa gcgggggaac gttcaccacg acgacggcga gccggaagta
    44101 aaggtcctgc cggaacccgc cctcctccgt ctcgcgcgaa agctcgcggt tcgtagcggc
    44161 gacgagccgg acgtcgaccg gcaggtcacg gctccccccg agccgccgca cgacccgctc
    44221 ctcgaggacc cggaggagct tcgactggag cccgagaggc agctccccca cctcgtcgag
    44281 gaagagcgtc ccgccggagg cctcctcgaa gcgccccgcg cgcgcctggt cggctcccgt
    44341 gaaggccccc ttctccgcgc cgaagagctc gctctcggcg agcgtctcgg ggagcgccgc
    44401 ggcgttcacc gcgacgaagg gtcccttcgc gcggggtgag cgggcatgga gacggcgcgc
    44461 gacgagctcc ttgccgacgc ccgactcgcc ggtcacgagg acggcgacgt cccgcggcgc
    44521 gacgcgatcg acgaggtcga ggagctccct catcgccggg tcggccgtca cgatctcgcc
    44581 gtcgtcctcg cgcggcagga ggctcgcgac gaggtcgacg agcgcctccg gcgacgggag
    44641 cggcttcgcg atgtagtcgg cggcgccgag ccggaccgcc tccaccgcct ccgggatcga
    44701 accgtgcgcg gtcaggacga cgaccggagg cggcgaggtg cgcgcgcgga cgcggcgcag
    44761 gagctcgagg ccgtcgaccg ccggcatcct ccggtcggtc aggacgaggt cgtacgtcgt
    44821 cctctccagg aaggcgagcg cctcggcgcc gtcccgcgcc tcggtgacgc cgtgcccggc
    44881 tccctcgagg atgtcgcgca ggagcgcgcg gaacgacggt tcgtcgtcga cgacgaggag
    44941 gcgcgccacg tcaggccccc tctccgggca gcgcgagcgt cgcgacgcag ccgcctcccg
    45001 gccgcggcgc gagtgaggcg gtgccgccgt tcgcgaccgc caacgacctg acgatggcca
    45061 gcccgaggcc ggtcccttcg ggacgggagg tcacgtacgg ctcgaacgcg ctcgccgggt
    45121 cgaggtcgag gcccggcccg cggtccgcga cctcgacgag ggcccggccc ccttccgcgc
    45181 ggaccgtcac ctccagcgtc ccctcggggt cgaacgcgcg ggcgttcgcg aggagctcct
    45241 ccaggatcgt ctcgacgtgc tcccggtcgg cgcgcgccgg gaccgcgcgg tcggcgcgga
    45301 cggccacccc cgccgcagtc ccctcgacgc cgcgcgaggc gacctcgcgg gcgagagcgg
    45361 cgaggtcgaa ggtggccggc tgcggatcgg gcgggcgcgc gaagtcgagg agccgggaga
    45421 ggatcgtctc catccgctcg ctcgccgtca cgatccgcac gacgcgctcc cgctcgccct
    45481 ccggcacgcg accgtcgagg agctgcgccg tccctttcac cactgcgagc gggttccgca
    45541 ggcgatgcgc gaggccggct cccgcccgcg cgacggtggc gagacgctga ccttcggcct
    45601 cggccgcctc cctcctctcg cgctcggcga ggccccgggc ggcgacgagc gcgaggccga
    45661 cgagggccag cgccgcggcc ccgccgccgg cgaggacgag cccggcgagg gccggctcgc
    45721 gccccagcgc gggcgccggc cgcagccgaa gccggatcgg cggcattccc cgccccgcac
    45781 ccgcccccgg acctcgcccg cccatgaagc cgcccccgga gcccggctcc gccccgagca
    45841 gggcgggcct ccactcgcgc ccgagcgccg cggtcacctc gtagccgtcc acggagtccg
    45901 cccccgcgct cgctaggacg ccgccgggac cgatgaccgc ggccccatcg acggacttcg
    45961 cgttgcggga aaggaactcc tcgaggaccg ccgtcgcctc ctcgggcgtt gcgccccgca
    46021 ggctcgcctc gagctcgtgc gccgcccgcg agaggacgcc gtcgcgcacc gccgcatccc
    46081 gctgccgcag cgtccgcgac gcgaggccgg ccgttccgag gagagccgac gcgaagacga
    46141 cggagaggac gacgatccag accgccgtgc ggttggagcg gatcacgccg tcatcgtacg
    46201 ggagtccggc ccggccggcg aggcggcggc ccgtccgcgc cgatagaatc cccgcgaatc
    46261 ccacgattcg cgaggaggaa ccgatgcccg gtatctacaa gaaggtcgag ctcgtcggaa
    46321 cgtcgcccgt cagcttcgcg gaggcggtca aggccgccgt cgaggaagcg tcgaagacgg
    46381 tccggcacat ggactggttc gaggtcgtcg agcagcgcgg ccgcgtccac gacggaaagg
    46441 tcgccgagtt ccaggtgacg atccgcgtcg gcttcaagct cgagcgctga gcctggcggg
    46501 gcggaccggg aggcccgccc cggcctctca gccgtggaac ggccggccgg tccgcgccgc
    46561 ctcgaggagg agcggggccg gcgtgaagcg cttgccgcag agcgtccgca ggcgctccat
    46621 cctcgcgacg acttccgccg cgccgaccgc gtcgacccag cggaacggcc caccccgaaa
    46681 cggcgggaac ccgaggccca tgaccgcccc cgcgtccgcg tcgcgcggag acgcgacgac
    46741 cccttcctgc aggcaccagg ccgcctcgtt gaccatgagg aggacgagcc ggtcgacgag
    46801 gtcggcggcg cgcatcggct tccgctccgt cccgccgaag tgcgcgtaga cgtcgcggtc
    46861 gacctccttt cgcccgccgg gccgggtgga gggtccgtac cggtagaagc ccttcccgtt
    46921 cttccggccg cgatagcccg cgtcgaagag cttcccgaac gtcgccgacg gcgctgcgcc
    46981 ccgcgcggcg aaggccttcc cgaggtcacg cgagacgtgg gccgcgacgt cgatcccgac
    47041 ctcgtcgagg agggcgacgg ggccgaccgg gaagccgaag tcgaggagcg ccgcgtcgag
    47101 gtccgggatc gccgcccctt cgtcgagaag gacgacggcc tcgttgacga acgggccgag
    47161 gatccgcgtc gtgtagaagc ccggcccgtc cttcacgacg atgaccgtct tcccctgcgc
    47221 gacgccgaac gcccgcgccg tcgccacggc ccacggggcc gacctttccg cgacgacgac
    47281 ctcgaggaga ggcatcttcg ggacgggcga gaagtagtgc atccccacga cccgctcggg
    47341 acgcttcgcc cctctcgcga tctccccgat cggaagcgcc gacgtgttgc tcgcgaagac
    47401 ggcgtcgggt gcgaggaccg cttccgcctc cgccaggacc ttccgcttca gggcgaggtc
    47461 ctcgaagacg gcctcgatga cgaggtcgca gcccgcgagc tcctcgggct tcgtcgtcgg
    47521 cttcagcgcc agccagcggc ggtccctctc gaggcgcgtc agcgccgcgg agcggagccg
    47581 ccggtcgagc cccttccgca ggctcctcgc ggcccgcgcg agcccctcct ccgagacgtc
    47641 cttcaggacg accggcgcga gcggaagcga cacggccgcg atcccctccc ccatcagccc
    47701 ggcgccgagg acgccgagcc gattcacggg gcgcggcgcc ggagagccct tcggcgcctt
    47761 cttcagcgcc gtcatcgcgt cgaagagccg gacgaggttc ttcgccccct cgctcgcggc
    47821 gaggcgaccg aagcggaccg cctcctcctc gaggcccgcc gcaaggccgt cggcgagccc
    47881 cttctcgacg caggcgagga tatcgagcgg cgccgcgtag aggccgcgtg tcttccgcag
    47941 gacctccgcc cgcgccttcc cgatcgccca ggcgcgaagg ggcgggaggg cgagcggcgt
    48001 ctcggcgatc ggccgcctgt cggtcggcgg cctcagcgtc cccttcgcga gcgcgagcgc
    48061 cgccttcgcg gccgtctcga ggaggccccc gggcgaggtg agcgcgtcga cgaggccgca
    48121 tcggtacgcc tgcgcggcgc ggaggcgctt gccggtcagg agcatcggga gcgcccgctg
    48181 aaggccgatg cggcgcggca gacgctgcgt tcctcccgcg gccgggagga gaccgagcat
    48241 cacctcgggg agggcgagga ccgttctcgg gtcgtccgtc gcgaggcggt agcggcaggc
    48301 gagggcgacc tcgaggccgc ccccgagcgc ggcgccgtgg atcgcggcga cgaacggctt
    48361 ccggctctcc tccacgcggt cgagccaggc gtgcgcgccg cgggagatcg actcggcctc
    48421 cgcggcgccc atcgcgagga cctcgtccag gtcggccccc gcgataaagc cgtcgggctt
    48481 ggcgctcgcg aggacgcagg cgaccaccgc gtcgtcctcc cggatctccc tcatgagcgg
    48541 gccggcttcg tcgaggaggc cggacgagag gacgttcacg ggccttcccg gcgtgtcgaa
    48601 caggaggacg gcgacgccgt cgggacgctt cttcagcgag aggcttctca tcgcgcgagc
    48661 accatcgcgt gcccgagggc gcctgccgcg cacgtcgtca cgacgccgat ctcggccccc
    48721 tcgcgctcca tccggtcgac gcacgtcgtc aggaggcgcg cgcccgtcgc tccgaacggg
    48781 tgcccgaggg ccagcgagcc tccccaggcg ttgacccgtt tcgggtcgat ccgccccacc
    48841 gcgtccttcc gtccgagccg ctcccggcag tagctccggt cggcgaggag cttcaggaac
    48901 gcgaggaccg gcgcggcgaa ggcctcgtgc acctcccaga cgccgacctc ccccgccggg
    48961 acgccggccc tatcgagcgc cttcgcgacg gcggcgacgg gcccgaggag gagctcctcg
    49021 agcggctcca tcgcgacgag cgcggtcgcc gcgacccgtg cccgcggcgc gaggccgagc
    49081 ctctccgccg cctccgcgct cgcgaggagg caggcggccg cgccgtcggt caggaaggag
    49141 ctgttccccg ccgtgacggt gccgaacgcg cggtcgaagg ccggggggat cgcgccgagc
    49201 ttcgcgagcg tcgtgtcgcc gcggatcccg tcgtcggcgt cgatcggccc gaacgacggc
    49261 ggcacccgag ccgggacgac ctgctcgacg tagagccccg ccctcgtcgc cgccgcggcc
    49321 ttgcggtggg agcggagcgc ccatcggtcc tgcgcctctc gcgtcagaga gagccgcttc
    49381 gcgaggcgct cggcgctctg ccccatcgtc tgccccgtcg agaactcggc gatcgcgggc
    49441 gtgtcggggg cgaggtcctt cagcgagagc ccctgcagga gcttcaccca gtcgaggacc
    49501 cccttcgcct tctgagatcg gatgaggcgc tttcgaacct cgcgtccgaa acggatcggc
    49561 gcgtcggaga gagtctcggc tccccccgcg atgacgacgt cggccgcgcc gctcgcgatc
    49621 gcctcgacgg ctccggagac cgcgacgttc gaggaggcgc aggcggcggt gacggtgaac
    49681 gcggggcacg acgccggcag ccccgcggcg agcgccgact cgcgggcgag gttcgtcgtc
    49741 cgcgggtcgg ccaggacggt gcccatcacg acgcggtcga cggcggcggg atcgaggccc
    49801 gtccggtcga ggaggcctcg gagcgccatc gccccgagct cgtaggccat cacgtcggcg
    49861 aacgcggccc cggcgcggag gaagggggtc cggcacccgc cggcgaagac gaccgttcga
    49921 ccgtgcacgt tcttcgcggc catggctcct ccctgtgcgg gaggaatgct accgccgaga
    49981 ccaggacacg gacgaacgcg gcgggttgag ccaacctcgc tctgcccaca cccgccgcgt
    50041 cccccggccg ctcaggccga ccggatctct cagtccgcgc ccaccttcga cgtgcggagc
    50101 gcgcgggctc ggcgcgccgc gatctcgccg acggaatccg ggaacgatgc ctgctcctcc
    50161 tcatccttgc tcgcacggcc gtacgcttcg atcgcctcgt cgaccaggcc gaagctctcc
    50221 cgaatgcgcc cgacgacgag ccagtcaccc ggcatcggtt cgtcgagccc ctggagctcc
    50281 atcgaccgaa ggacgaggga atgggcctcc tcatgtctcc cgagctcggc gcagatggcc
    50341 gcgagcgtgt tcaggatggc ggggttcgag cgccgggagc cctcgacggc ctgccgcgcc
    50401 aggctcaggg ccttctcatc gaccttcccc gcgacgtacc gcgcccaggc ggaggtgttc
    50461 gcgtcgttcg ccgacggagc gggcctctgc atcaacttgt ccgcggcctc gagcgccccc
    50521 gtcacgtcgc cggaggtcgt acggagcgac acgagcgagg accccacgag ccggcagctc
    50581 ggatgcgacc gggagtgctc ttcggcctcg gcgagcgcct cctcgactcg tccgacccgg
    50641 gctttcagcc cgatgtcgaa catcgcgacc gactcggtgg aggctcgatc ggcgcggatg
    50701 accggcagga gcccgatcgc ctcctcgagg agcacctggg tttccttctt cgttcgcttc
    50761 gagtcggggc cggtccgcgc gacgagcgac ctatggagga gcgcggcgcg gagggggtcc
    50821 tccggcttct cgcgttgcgc acggcgcagc tcgtcgagga gtggatccgg gagcggcgtg
    50881 ccggcgaggc cggcgagggc cgcgagccga agctccgccg cggacgcccc ctcggccggc
    50941 cgcagacgcg gcaggagcgg gtcgaggacg agatcgctct cctcttcgtg cacctcgacc
    51001 tcgagcgccg cacgctcgag ccaccggcgc gcgtccgcga cccggccgcg cgtcacgagg
    51061 tcgagcgcgt accagcagag gaacctcgag ccgccgtcgt cggagacgac gcgcgggacc
    51121 ccgtcttcct ccgccaccca gacccggatc tgtcggggcg cctgccccac cgacgggacc
    51181 gtacccgaaa cgagccagcc atccgccgca cttccctcgg ccgagagctc cgtcgccagg
    51241 gcggtgtccc ggaggaacgc gagcgccgct cccgacgaga gggcggagcg acggatggtg
    51301 gccacctcgt cctcgaggaa cttcctgagg agcgacgtcc cgtgcgcgtc ccgcgcggcg
    51361 cgcacggcct tcacgtagag cttctcgacc gactcgatcc ggccgtcccg agggaagagc
    51421 tccgccgtga gggcatggac ggccgacagc gcgttgcccc ggaccggggc cgggacgggg
    51481 ccgtcgtggc gcttcaggcg gcgggccgtg ccggcatgga cggcggcgac gccggcgccg
    51541 tcccctccac gggctccctc gtccatcagc gcggctccct cgggatagcg ccggaggacc
    51601 aggaggaggc tgccgatctc gccggccgcc ttgcgtcgcg ccgccggctc cggaaccgcc
    51661 tgccgaagca gctcgagggc acgcgcggtt ccccgggagg ccgcgacggc tgcgatccga
    51721 aggccgatca tctggtccac gtcgggccgc ttctcgagga acgcggcggc gtcggccgga
    51781 cggtcggcgc gcagcagcgt gagggcgtag ttgcgggcga cgaccgtcgc gtccggggac
    51841 ttctcgacga gcttcgcgta ctcgtccaga gcgagcgcga gccgggcgga gtcggcgtag
    51901 cgatatccct cgcggtcgtg ctcgagcagg atcgcgagct cgaaccgcac ctcgatgtcg
    51961 tcgggatcga gctcgaggga cttccgaagg gcggcttccg cttccacggg gtcccacccc
    52021 ttctggcgca gacgacccca ccggtcctgc tcgaggacga gcccgagcag gtaccaggag
    52081 cgggccgaag ccggctcctt cttcacggcc tcgcgcgcct cccggcgggc cgcgtctccc
    52141 aggccggagg ccaggtagag gcgggccagc cggcggtgcg gggccgcctt tgcggggtcg
    52201 cggcggatct cctcgcggag gatctcgagc ccgcgcgcga cgtcgccccg ggccgcggcc
    52261 accgaggagc cgtgctggaa cgtgaacgcc atcggctccg cctcgcggag ctcggcgagg
    52321 ccctcgcgca gggcgacgac ctcggccggc ttcagccggg cgcgcgtcag ctcgacctcg
    52381 acctcgaacg tgacgcttcc gtcctcgcgc gccacggcct tccggtgata cgtcgccggc
    52441 ccgaggctcc gcgtctcctc ctcgggaagg agcaccggtg agaagccggg cggagggacg
    52501 aacgagtacc ggtactcctg gcggaacggt agggccggga gctcgttctt ccgctccccg
    52561 tcctccgggt caggcagctc gctcaggaag ccgtcggccg cgatcaggag cgagatgcgc
    52621 tcgtcgtccg cgcggacgaa cgcgggccga gccacctccg tctcgagggt gaaggtcgtg
    52681 gagaggtcgc gcgagggcga tacgacgccc cgcaggacct tccctcctcc gaagcgggat
    52741 tcggcccagc gctcgaagga ggacttcagg tccgactcgc cggtcccgtc gtaggcctgc
    52801 ctccggttcg cctcgaagac gcccgagtag accgtcttct ccgatgccgt cgcctcgccc
    52861 gcctccggaa tcctcacgac gatctccgtg cgggaggtgt tcgaggcgga cggcgcgatc
    52921 gggatgcggg acggcccctg cgtctccgga cggcagacga ggacgagccg gccctggagc
    52981 cgcgcgggga cctccccgag cctctcgaac cgggaggtcg tgtcgacgaa cacaccctcg
    53041 ggccggagcc cggcgacctg gacgattgcg tgatcgaagg cgtccagcgt gggcagcgcg
    53101 ggggtgacgt ccgggtcctc gccgacgcgg aggagagcga cggccgcgtc gaagccgcgc
    53161 aggcgcaggg ccgccacgag cagggtcgcc tggtccttgc agtcgccgaa cttccgacgc
    53221 agcacctcgg ccggcgaaga cgggacgatc gccccgtcgc cgaagaggag gcccgtgtac
    53281 cggacttccc gtcggaccca ctccgcgagc gcggccgcct cgtgcagtcg gctcgggaag
    53341 ggccccgccg gcaacgacgc ggcgtcgagg ggaccttcgc cgatacgttc ctcgacgacg
    53401 gcccggtagc tcgtcgccac ctggttccag gacgtgcccg tcgagaactc gacgagcgga
    53461 ttcgcgggga cctccggcgg gaggtcggat tcgacgtcct cgagcggcgg gacgttccgc
    53521 cgctcccaga cccggacgac ccgaccggac gcgagcgagg ccttcggtgc gaggtcgatc
    53581 ccgatggcct tccagacgaa cggcagggtg gagggggcct ccacccggca gcggatccgc
    53641 ccggccgggt aggagtaggc gaggagctgg ctgccgaggg tgcccgtggg cggcgcgctc
    53701 gtcctcgtct tcacgatctc ccactcgacg accgcgccgg gctccatccc gggcagaggg
    53761 acccggagga ccttccggtc gtcgaacagg agcgggtcgg acgacgccgg agactccgcg
    53821 atcgtcttct cgtcgaggac gaggaccgtg ccgtccttgc ggatgacgcg ggcccggagg
    53881 atcggcctcg cctcgatcca gggtttccag ctcgcggcga ccgatcccat ctcctccgcc
    53941 ccctcgtgcg agaggacccg gaggacgcgc cggcgggcca tggagaaccg gccgtccgcc
    54001 tcgaaggtcc agcgggactc gtcgaggaga agcctgtacc aggcggagtt ctcctccgtc
    54061 tcgccgggca gcttctcctc ggacgctcga aggacggccg cgggttccgc ctcgaacggc
    54121 gcttcgagcc agggctcctc accccggacg ggaacggtcg cgaggggaag acatacgagc
    54181 agaatgcgag cgaatcgacc ggtcacggcg cctcccgtcg atgcgtccgg gagatgcctg
    54241 tgccgccgcc cgaacgctga atccggtgat tatgcctgct cacctctcgt gcagccgtcc
    54301 ggcgtcgcat gcggcgcgtc gacgcgcccg ggtcctcgcc gcctctcgag cggcgattgt
    54361 ccgtcgccgt cagttcggcg ggtcgtacgc ctcgccggcg acgtagctcc cgtcgccggt
    54421 cctctctccc ggggcggcac cgtcgttcag gacgccgtag gcggcgaacg ttccgcccgc
    54481 cgaggctcgg cggacacgga cgaaaccctc ggtgacggcg gtgcctgaaa ggacgcggtc
    54541 gacctgactc cagccgagcg gcggaaggtc gaaggggacg gaggagagga ccttcgcccc
    54601 ggtcgctccg tcgtggacgt cgtacgccac ggagacgggc accgtcgtcg acgggttgaa
    54661 gagcgcgagg ttcgagcggg tcgtcggggt ctgacggagt ccgtcgacga ccgcctcgac
    54721 cgccgcggcg gacgtggccg gcaccgcggt gtagaagagg ccgaagctcc ccttcccggc
    54781 gcatgccccg ctccccgcgg cggcggtctt cgcaccggcg aggccaggga cgagcgtcgt
    54841 gaacggggac tggaagagga cgaggagcgt cccggcgtag gacgcggcgc cgcgggggcc
    54901 gagcgggagg ccgaggcccc ggagcgtgtc gagggcccgc ggaaggatgc gctgctggaa
    54961 cggcccgagg tcgatcgaga ccgctccgac ccgccccttc ggggtcgcga gagactcggt
    55021 gtagaccaac gacacgctga cggcctccgc cccggggttg aagaggacga gctcgctctc
    55081 gaagaccggc gtctcgacga cgaccggcag gacgagctcg ccgccgaacg cgtcgtccgg
    55141 gacggccgga acgaaggagc cgtcgtgcga gaggttgtcg ttgaagacgg cgtaggtgcc
    55201 gagcggcgcg gtgccggccg cgcggctcac gagagcggtc gcctcggcga agcccgcggc
    55261 ccccaggagg tccgcgtcgt tcacctgccg ccactcgccg ggggcgagcg gcggcaggag
    55321 agcggccggg acctcataga cggaggcgcc gtcgcccgag cgcaggacga ttcgcggggt
    55381 gacggtcccc tccgtcccca tgttcacgac ggcgaggttc gatcgttccg ccgggccgct
    55441 tcgaagaccg aagagccgga ggggcgcggt cgagccgtca cccatcctcc acgcggcata
    55501 agagagcccc gcggcgccgc cgcaggccgc ggccgtcgtc cgcacgcggg cggagacgac
    55561 gtcggcgtcg ggcgaaaggc ccgtgaagac gaagcggagc gagccggcct gccccggacc
    55621 ctcggggatc gggagcccct tcagcgtccg gagccacgcg atcgcgtcgg cgatcacgcg
    55681 ctgctcaccc ggcgcgaggg tgtcggcgac ggtgccgcct cccgcggctc ccgggaacat
    55741 ccccgtcgcc ggcatgtagg tcgcctcgac ccgcgccgcc gtcgtcccgc ggttcgtgag
    55801 cgtcagctcc gagaggaacc gggcctcgcc gacgccccgg acgtcgagga cgacgggaac
    55861 gaggagctcg gcccgcgccg tccccgccgg gatcgaccgg agcgcggcga gcgccgccgg
    55921 cgcgtcgagg cggttccggg tcacgccgtt gcgcgcgtcg aggatcgcct tcccggtctc
    55981 gcgcagcgcg gccttcacct cggtcggccg gcggtccggc tcggcgccga agaggagcgc
    56041 cgcgacgccg gcggcgtagg gggccgcggc cgacgtgcct ccgaagcagg tgtgggtccc
    56101 gcctccgttc ctcggcgccg tcgcgcagtg cgccggggcc aggacgtcga gacggtcgcc
    56161 ggtgtcggag tagcaggcga tcgcgtcggc cgccggcgcg gaatcgctgc agcccgaggg
    56221 gaggcagtgg cctcgtccga aggcctgcgg gctcacggcc gcgtcgtaga cggccccgac
    56281 gacgagcgcg ttgctcacgc aggcgggaag gacgacgccg ccggtgcagc cgccgttccc
    56341 gctcgcggcc acgacgagga tccccgcctg cccgagcagg tcgaaggccg ccttgtagtc
    56401 gggggcgagg aggtcgcagc ggcccggctc gggaggcgcc tcgccgtcga agtagccccc
    56461 gagggagagg ttcacgacgc ggatcgcgtg gggattccct tcggcgacgc tcgcgaggac
    56521 ggcgtcgatc ccctcgagga cctgcgcgtc cgacccctcg ccccgcgtgt cgaggacccg
    56581 caccgcggcg accttcgcgc cgcgcgcgac ccccgtcgtc aggcccgcga tgatccccgc
    56641 cacgggcgtc ccgtggccgt agccgtcgcg tgcgtcgccg tcgtggtcga tcgcgtcgta
    56701 gaggttgacg accttcgtcc cgagcgggag ctcggggtgg gtgtagtcga tcccgctgtc
    56761 gaggaccgcg atcgtgacgc ccgcaccgtc gacgcccgcc gcccgaagct cgggcacccg
    56821 catcagtccg tctccctcgg tgtcgagggc gcggacgcgt ccgttcgggc ggacccgctc
    56881 gatgcggggg tcgccggcga gggccgccgc ggccgcccgc ggcgcgcgaa ggaggagcgc
    56941 ggggacgaac gcgaaggtcc gctcgacccg cacgccgaac ggcgcgtagt cgagctcgag
    57001 ctcttccgcg aggcgagtct ggaaggccct cctctcacgc ttccgctgcg cgtcggggcc
    57061 gacgcgccgc gcctcctcgg gcagccggag cgtgacgagg agctcgacgg ctccctcgcc
    57121 cgatggcgcg ggcgccagga ggcgcgggtc ggccttctcc agcgggccgc gcacgtcggt
    57181 gcgcggccgg agccactccg cggggaacgg cggcgtcgcg tccgccggcg aggcggtcgc
    57241 gagcacggcg aagacggcgg cgaggagcgg cttcatggtc cccgatcgta agtgacggag
    57301 cccgcgccag ccccacgggg cgctcccccg cacggcgacg cggtcagcgc ctgcgaagga
    57361 cgaccgccgc ggccgttcct tcggcgaacg tcgcctcgac ggcgtagtcg cgcgcgaggc
    57421 gggcggcgat ccgcgtgccg tagtcccggc cgaaggcgga acgcccgaac tccgaggtgg
    57481 gccgcgagac gacgacgacc cgcgccggcc gcgcgcgatc cagcccgtcg gcgaggtcac
    57541 ggtcgacccg gtcgtccacg cagccgggca gcacatgctc gaaccggagc ggactccgca
    57601 cgccgaggag gaacgagagc gcccccgcct cggggaggac gacgaggtcg cggtccttca
    57661 ctccgacgct ccggaggtgg gccgcgacgc gggagaaaag cgccccctcg tcgccgggcg
    57721 ggaaccaccg cccggcggga cccgccacct cggtccgcgg cgcgcgatag aactcgacga
    57781 gccgggggag gaacaggacg ggcgcgagga ggagaggtac gaccgccagg agcgctcccc
    57841 ggcgacgcgc ctccggaggc gccgccgccg ggatacggca cgcgaggagc cacgccaccg
    57901 cggggagagt ggagacggcc ccgagcggcg cgtagggata caccgggacg gtccagagcg
    57961 ccgtcctcca cgcgaacgcg aggccgacga gcgacgccgc gagcgggagt cgcgcctccc
    58021 tctcgccacg gcgaaggtcg accgccgcgg cgacggacgc gagcgcggcg aggagcggaa
    58081 ggccgcggac cccggtcgtc acgaccggct ccctgaggac gaccgcgccg acgaggaggc
    58141 cggccagggc gagcgcgcct ccggcggccg cgcgtgctcc ctcgcgtttc gcgagcgccg
    58201 ccgcgcagag ggcccagcca ccgagcagcg ccgcgccggc caggagcccg ccgacgccgc
    58261 gaggaaggag ggaggggtgg aggccggaga cccgaaggta gagctcgcgg aactccggcg
    58321 gcatgacgaa gtggcggagc gggccgtacg aaacgaggtc gccgacggga attcccgcca
    58381 tcgcgaagcc gtagccggca gccgagagga cgcctgccgt ccccacgagc cgagcggcgc
    58441 tccgccacgt gcctgccgcg agcgcgacgc cgaggagggc gggaagagtc tcgggcttgg
    58501 cgaggagggc gagcgcgccg aggagcccgg cgagagcggc acgcccggag gcggccgcga
    58561 ggaacgcgcc ccagacgagg ccgaccgcga ggagcgcggc catcgcgtac ggagccgcga
    58621 acgctccgtt ctcgggcgcg aacgcgagga cgcccaccgc gacggccgcg gcccccgcgt
    58681 tcgcggcggg cgacagcatc cggcgcccgg cggcgaggag cgcggcgagc gtcgccgcgg
    58741 ccgagaggag ggcgaagccg acgtacgtcg ccaccgtcgc cccaaagagc cggaacgccg
    58801 ccgccaccgc gtgcggcgcg agcggcccgt agtagtagcc gacctccgac caaagccgct
    58861 cccccgccgc caggcgcatc gggacggtcc attcgcggtt caggtccccc tggaacgagg
    58921 cccagcgcag ccacgagagc gccgtcagcg agacgaaaag cgccacggcg aaggcggcgt
    58981 ctccccgccg gaggagccgg tgcgtcggca tcacgcgatt ctctcccggt gtcgcctgat
    59041 gaaaacacat cagtcgagat cccgtcccag gaacattccg gtgcctttac ccgattccaa
    59101 ggaacgcgcg cgagagagat gatgcacggg ccgcggcggg ccctacgctg cccgcgcgcg
    59161 atcccacagg aggatgtctt catgacgacg agaccgatgg tcaccctgga cgggaacgag
    59221 gcagtcgcct cggtcgccca cagggtcagc gaggtgatcg cgatctatcc gatcaccccg
    59281 tcatccacga tgggcgagtt ctccgacgag tggtccgccc tgggccgcaa gaacgtgtgg
    59341 ggaaccgtcc ccctggtgtc ggagctgcag tccgagggcg gcgcggcggg cgcggtccac
    59401 ggggcgctgc aggccggggc gctgacgacg acgttcacgg cgtcgcaggg gctcctcctg
    59461 atgatcccga acatgtacaa gatcgccggc gagctgacgc cgttcgtcat gcacgtctcg
    59521 gcgcggtgcg tcgcgaccca cgcgctctcg atcttcggcg accactccga cgtcatggcc
    59581 tgccgccaga ccgggttcgc catgctcgcc tcgagcaacc cccaggaggc gcacgacctc
    59641 gccgcgatcg cgcaccgcgc gacgctgatg gcgaaggtgc cgttcctcca cttcttcgac
    59701 ggcttccgca cctcgagcga ggtcgccaag atcgagctcc tcgggaacga cgacctcctc
    59761 gcgatggtga gcgaggacct cgtctcggcc cagaggaagc gggcgctgac gcccgaggcg
    59821 ccggtcctgc gcggctcggc ccagaacccc gactgcttct tccaggcccg cgaggcgtgc
    59881 aatccctact acgacgcctg cgccggcaag gtgcaggagg cgatggacgc gttcgcgaag
    59941 ctgacggggc gcgcctaccg cctcttcgac tacttcggcc accccgaggc cgagcgggtc
    60001 atcgtcctga tgggctcggg cgccgacgtg acgcacgaga ccgtcgagta cctcctcgcc
    60061 cgcggcgaga aggtcggcgt cgtcaaggtc cgcctctacc ggccgttcga cgcgtcggcg
    60121 ttcctcggcg cggtcccgaa gaccgcgaag gcgatcgccg tcctcgaccg gacgaaggag
    60181 cccggctcgg tcggcgagcc gctcttccag gacgtcgtca ccgtgatgcg gaacgcgcgc
    60241 gaggacggca cgtcgccctt cgccgtcgag ccgaaggtcg tcggcggccg gtacggcctc
    60301 gcctccaagg acttcacgcc ggcgatggtc aagggggtct tcgacgagct gaagggtgcg
    60361 aagccgaagc gcggcttcac cgtcggcatc gtcgacgacg tgacccacct ctccctgaag
    60421 tgggaccccg acttcgacac cgagggcgac gacgtcaccc gcggcgtctt cttcggcctc
    60481 ggcgccgacg gcaccgtcgg cgcgaacaag aactcgatca agatcatcgg cgaggagacg
    60541 gacaactacg cccagggcta cttcgtctac gactccaaga agtcgggcgg gatcacgatc
    60601 tcgcacctgc gcttcgggcc gaagccgatc cgctcctcct acctcgtcaa gacggccggc
    60661 ttcgtcgcct gccaccagtt cggcttcctc gagaagtacg acgtcctcga gtacgccaag
    60721 ccgggcgccg tcttcctcct gaactccccg tacggccccg acgaggtctg ggacaagctc
    60781 cccgtcgagg tccagagcga gatcctcgag aagaagatcc gcttctacgt catcgacggc
    60841 tacgaggtcg gcgccaagac gggcatgggc cgccgcatca acaccgtcat gcagacctgc
    60901 ttcttcgcga tctcgggcgt cctcccccgc gaggaggcga tcgcccagat caagaagtcg
    60961 atcgagaaga cctacggcaa gaagggcgcc gagctcgtca ggatgaactt cgaggcggtc
    61021 gaccagacgc tcgagaacct ccacgaagtg aaggtccccg ccgccgcgac gtcgaagctg
    61081 aagaggccgc cgatcgtccc ggaggaggcc cccgacttcg tccagaagat caccgcggtc
    61141 atgctctcga acaagggcga cctcctcccc gtctcggcct tcccggtcga cggcaccttc
    61201 gccacggcga cggcgcgctg ggagaagcgg aacatcgcca tcgagatccc ggtctgggac
    61261 gagaagctct gcatccagtg caacaagtgc gcgatggtct gcccgcacgc ggcgatccgc
    61321 gccaaggtct tcgaccccgc ggtcctcgag aaggcccccg ggacctacaa gtcggtcgac
    61381 tacagggccc ccgagttcaa ggggatgaag tacacgatcc aggtcgcccc ggaggactgc
    61441 acgggctgca ctctctgcgc cgcggtctgc ccggccaagg acaaggcgaa cccgaagcac
    61501 aaggccctcg acatggcccc gcaggcgccg ctccgccagg ccgagcggga gaacttcaag
    61561 ttcttcctcg gcctccccga gcccgaccgg acgaagctga aggtcgacgt gaagggctcg
    61621 cagttcatgc agcccctctt cgagttctcc ggcgcctgcc tcggctgcgg cgagacgccc
    61681 tacatcaagc tgatgacgca gctctacggc gaccggctcc tgatcgccaa cgccactggc
    61741 tgctcgtcga tctacggcgg gaacctcccg acgaccccct acacggtcaa ctccgagggg
    61801 cgcggccccg cctgggccaa ctcgctgttc gaggacaacg ccgagttcgg cttcggcatg
    61861 cgcctcgccc tcgacaagca cgccgagcac gcggccgagc tcgtgaagga gctctccggc
    61921 ctcgtcgggg acgagctcgt ctcctctctc ctgacggccg accagtcgac cgagcagggg
    61981 atcgcccacc agcgcgagcg cgtcgcggcc ctcaaggcga agctcgccgg tcagaagggc
    62041 ctcccggccc gccgcctgac ggacatcgcc gactacctcg tcaagaagag cctctggatc
    62101 gtcggcgggg acggctgggc ctacgacatc ggctacggcg gcctcgacca cgtcattgcg
    62161 atgggccgga acgtcaacat cctcgtcctc gacaccgagg tctactccaa caccggcggc
    62221 caggcctcca agtccacccc gatcggcgcc gtggcgaagt tcgccatggc cggcaagtcg
    62281 gccggcaaga aggacctcgc gatgatggcg atcgcctacg gcaacgtcta cgtcgccaag
    62341 atcgccttcg gcgcgaagga cgcccagacg gtgaaggcct tccaggaggc cgagagctac
    62401 gacgggccgt cgctcctcat cgcctacagc cactgcatcg cgcacggcta cgacctctcg
    62461 ctcggcctcg agcagcagaa gctcgcgtcc gagtcgggct actggccgct cttccggtgg
    62521 gacccgcggc gggtcgccga gggcaagccg ggcctcgtcc tcgacagctc cgccccgaag
    62581 acggacgtcg gcaggttcat ggccaacgag acccggttcc ggatgctcga gaagacgaac
    62641 cccgagcggg cgaagatgct cctcgacctc gagaggcacg aggtcgcctc gcgctgggcg
    62701 atctacgaga acctcgcccg cggagccgac cccgtcaccg cggtcgccgc cgctcagacc
    62761 actccgaagg ccaactgagg acgaggagcg acaacgatgg atctctcgac cacctacctc
    62821 gggctcaagc tcccccaccc gatcatgccg ggcgcctccc cgatggtcga caagatcgac
    62881 ctcgtcaagc ggatggagga cgccggggcc gcggcgatcg tcatgcactc cctcttcgag
    62941 gagcagctca tgagggagga gctcgcgacg taccaccaca tcgacgtcca cgccgactcg
    63001 ttcgccgagg cgatgacgta cctcccccga cccgacgagt tcaacctcgg gccggaccag
    63061 tacctcgagc agctcgtgcg gatcaagcag tccgtcgacc tgcccgtgat cgcctcgctc
    63121 aacggcttca cgtcgggcgg ctggatccgc tacgccaagc tgatgcagga cgcgggcgcg
    63181 aacgccctcg agctgaacgt ctactacctc gccacgaacg ccgacgaggg gggcgacgag
    63241 gtcgagcagc ggacgatcga catcctgaag gcggtcaagc aggccgtcac gatcccggtc
    63301 gcgatcaagc tctccccctt cttctcctcc atggccagca tggcccgtcg cctcgacgag
    63361 gcgggcgccg acggcctcgt cctcttcaac aggttctacc agcccgacat cgacgtcgag
    63421 agcctcgagg tggagccgac gctgaagctc tcggactcct ccgagctcaa ccttcggctc
    63481 cgctggctcg cggtcgtctc gggccgcgtc cgcgcctcgc tcgcggtctc gggcggcgtc
    63541 cacacccacc tcgacgcggt caaggcggtc atgaccggcg cccacgccgt ccaggtcgtc
    63601 tcggcgatcc tccagcaggg gcccgacgtc ctccggcgga tccgcgaagg gctgacgcag
    63661 tggctcgagg agcacgagta cgactcgctc gagcagatgc gcggctcgat gaacctcctc
    63721 cgctgccccg acgcctcgat gttcgagcgc gcgaactaca tccgcatcct tcagggctac
    63781 aaggtctgac cgccgggctt ccgacatcgg acgaaggggg cggcccacgg gccgccccct
    63841 ttcctttttc ggcgccggcg cgcgggcgga cgcttcctca gccgagaaac ctcagttcgc
    63901 gcgtaagtga atgcagtcac gcacgattcc aactgtggcg cagttgaaac gggtcacgcg
    63961 aagtgctgca caggcaaagc gttaccaccg aaagcgtcgt caactggggt ttctcggctc
    64021 aggggcggac gttcacctga agccagtagg cggccatctc gcgcagcgtc ctcgcgtgct
    64081 ccgcgaactc cacctgcttg acgacgaagc tgttggcgcc cgcctggtag caggcgcgga
    64141 cgtccgccgg cagcatcgac gaggtcagga cgacgaccgg gacgtcccgg cccctcccgt
    64201 cggcccggaa acgcgtcagg acctcgatcc cgtcgacctt cggcagcttc aggtcgagca
    64261 ggacgagggc cggcggcggg accgtcccgg cggcggcctc tccccgcccg aggaggacgt
    64321 cgagagcctc ctccccgtcc ctcgcgcgga cgacccgggg agccagctcg catcgcgcga
    64381 gggcgcgcag agccagctcc gcgtcgtcgt cgtcgtcctc gacgaggagg atccagacct
    64441 cttcaggcat cgctctcccc cgtttccgga agcgagaacg cgaaggtcgc cccctggccc
    64501 gggcgtcctt cggcgtgaac gctcccgccg tgtctctcga cgatccgccg aacgatcgcg
    64561 aggccgatac cggtcccgtc gtccccctcg ccggcgtgaa gccgttcgaa gacgcggaag
    64621 agccgtccgg cgtgggccgg gtcgaacccg acgccgtcgt cgacgacctc gcagacgacg
    64681 tgcccggcct cgactctccc tcggacctcc acgctcctcc cgggccccgc ggcggcgtac
    64741 ttcaccgcgt tcgcgaggag atgccggagg acgacgcgga ggagcgcggc gtcgccccgg
    64801 gcggtcggca gatcaccgac cacgaagcgc caggcctcgc gagcctcggc cgggaccacg
    64861 tcctcgaagg cgccccgcgc gagcacgccg aggtcgacgc gttcgtgacg gaggccggcg
    64921 gaccccgccc gcgcgtactc gacgaggtcc cggaggagcc ggtccatccg gcgcgcgccg
    64981 ccccgcaccc gcgcgagaag ccgccggccc tcgtcgtcga gccgggcgga gtgctcctcc
    65041 tcgaggatcc ccgagaaccc ctcgatcgcc ctcagcgggg cgcggaggtc gtgagcgatc
    65101 gagtggacga acgcctcgag ctcggcgttc ttggcggtca gctcggccgt ccgctcggcg
    65161 acccgccgct ccaggccggc gttcagctcc cggatctccc ggtcgctgcg ctggagagcc
    65221 tcgcgggcgg cgtagcgtcc cgtcacgtcg tgcacgaccg acaggacgtg cggctcgccc
    65281 ctcacgtcga ccctgcgcgc cgagacgctg ccgacgaacg gagtcccgtc gcggcgccgt
    65341 ccctcgagct cgaggtcccg ggtcgcgtcg gactcgagcg cccgctcgaa gacgcggtcg
    65401 cgggcccgcg ggtcgaccca gacccccagc tccgtcgagg agcggcccac gatctcgtcc
    65461 cgccgatagc ccgtgagcgc ctcgaagccc tcgttcgcgt cgcggcagat cccgtccgac
    65521 acccgcgtca tgaggaccgc gtccgggatc gtgtcgagga gtcggcggaa gtggtcgcga
    65581 gcctcccgga cctccgcgtt cacgcgacgg ttcaccagga tgacgatgcc gaaggtcacg
    65641 aggttcgtca gaaggaaccc gtcgacgtac ggcgcgacgt tgaacgcggc gctcccccag
    65701 acgtcgccga cgtgggctcc gataaggagg accgcgccgc ggtaggcgaa gaacgccgcg
    65761 tgggcgagga ggcccgccac caggacgaga gcgacggggc ggctctcgcc cgtccgttcc
    65821 cgccagagct cccgcgccga gaggagcgcc gagacgacga gcgccgcgga gagggcgacg
    65881 gcccgaaggt cgatgtccgg gacgacccag aggaggaacg cgtgcgccgc gaggtaaggg
    65941 acgaggacgg ccccgaagag gcgccggtcc tcgtcccggc cgtggaagcg gcagaggccg
    66001 gcccagaggc agagcgtccc gaagacgatc aggccgttct gcgcgaagat cgcgacgcgg
    66061 ccgagcgtct cgtggtcccg gaagaggaac gcgaccgacc cggcgccgac ggcagcgctc
    66121 cagaggagaa atgcgccggt gccccggtag ctccgaaaga gcgcgtactg gaccgagagg
    66181 acgacgacct gcagcgcctg cgtcagggcg aagagcgcga ggagcgtgcg aacgtcgagg
    66241 gtcacgatcg ccggcccccc gagccccgag ccaccccgac agccttcgag cgtcgcttca
    66301 cgccctctga ttatgagcgc gcggggggcg ccggctcccg tgcggccttg cgttcggcgt
    66361 ccgcgaggag gtctcgggca tgctcggcca ggggcgagcc gggagcccgc tcgagcagat
    66421 agcggtagac gcggaccgcc tcgagcggcg ccttcgcctt ctccaggtga tgctcggcca
    66481 cgcggagcag ccccttgaac gctttcgcgt cgcccggctc cgcgtccagc gtctgggaga
    66541 aggtccaggc cgcggcggcg acgtccccgt cgcgaaggag ctggtcggcc agcagggaga
    66601 gggccgggcg gtcgagaccg agggcgtcga ggcgatcgag gtgctggcgg tagatcgccg
    66661 cggccgcctt cggcgagccc ttcgcgaggc cggcgggaat ggccatccgc gcggcctcga
    66721 gcgcgccctc gacgtctccc gcggtcaagc gcgcctgcca gatcgcctgg agcgactccg
    66781 ggtcggcggt cgaggcctcg gccggcgctg cgcccgccgg cgcgacggag gccgtcccgg
    66841 cgtgtgccgc gacctcgacg tcgagcggct cgtcggcctc ctccgcgagg ctcgcggcgg
    66901 cgaaggcgcc gcagagcttc ccctggagcg tcagcaccat cccgacggcg tagagcagaa
    66961 gcgggacgaa cgcggtaagg gcgagcttcg gctgccgcgg catgagggcg ccgtagaccg
    67021 gcaggacgac gaggaggacc gtgagcggtg gcccgaagac gccggcgatc gcgaggaaga
    67081 gctcgccgat ccggccggca aagagcgtcc gccagagcgc aggcgagaag agcgcgccga
    67141 agctgggggc gaccgcggcg acggcgacga acgcgaacga gaggacgacg ccgaggccgg
    67201 accagagcga gatgaggaac ggaaggcgcg gaaaaccgat cgggacgagg ccgatggccg
    67261 ggtccggcgt tcccgccccc ttcagcagcc cccagacgac gaacggcacc gcccaggcgg
    67321 cccacaggag aaggagccgc gcggccgccg ccatcgtgcc gctccagggt cccgccgcgc
    67381 tcgagaagag ccccccctcg gtgtccccgg cgacgccgac ctgcgcgaag cgcgagacga
    67441 agacgaggac ggtccccatc cagacgacga ggaccgcgac cgcgtccccc ttcgtgcggg
    67501 ggccgctcag cgcgaagagg aggacgatcc cggtcgggag cgcggcgagg agccccgtga
    67561 agaacgaggt gatcgagaag gagtcgtgga cgagctcccc gaagacgcgc gggaacgccg
    67621 tgcggctggc catgagacct cctgccccgt tccccggggc ccgggacgct ccggatcgat
    67681 gccccgattc tagggggacg gagctccggg gccatctgct ataaagccga ctctcgtagg
    67741 gatcccatcg aaggaaccac gaaggagaca cgcgatgaag ctccgtccga cccttctcct
    67801 cgccgccgtg gcgctggccg cgccggcgtt cgcccagttc gagaccccgc agccgagccc
    67861 gaaggcgacc ctgagccagc gggtcggcct gaccgacgtc acgatctcct acagccgccc
    67921 gagcgtccgc ggccgggcca tctggggtga cctcgtcccg tgggacaagc cgtggcggac
    67981 cggcgcgaac gccgcgacga ccctcacctt ctccgacgac gtcacgatcg aggggcagaa
    68041 gctcgccgcg ggcacctact ccctcgtgac gatccccggg aaggaggagt ggacggtcgt
    68101 cttcaacacc gacacgaagc tctggtggga gacgcagtac gacgccgcga aggacgcgct
    68161 ccgcgtgaag gtgaagcccg aggccgcgcc gatgaccgag acgctccaca tcgggttccc
    68221 ctcgatctcg gcgacgggcg ccgtcctcgc gatcgagtgg gagaaggtcc gggtccccgt
    68281 gaagctcggc gtcgaggtcg acgggaagct gaagaaggcc ctcggctccg ccgaggcgag
    68341 cgcctggcag tcgcccctga tggcggcgcg ctactactgg gagcagcggc aggatcgcgc
    68401 ggaggcctgg aagcagctcg agaagtcgat cgccgcgagc cggaactggg cgaacgtctc
    68461 ccggaaggcg cgcttcctcg ccgaggaagg gaaaacggcc gaggcggtga aggcgggcga
    68521 ggaggccctc gccctcgcga agaccgaccc ggcgaagccg aacaccgcgg cgttcgagaa
    68581 gacgctcggg gagtggaagg cgaagaagta gccacgatcc ggcctcgggt attcgcgacg
    68641 cgggccgaga ggcccgcgtt cgcgtctcgg cccgcgtcga cgcccaccgc ggcctccccc
    68701 gcgccgcgcc gaagtccggg ccggtcatcc ggtcgtagag agcccggaga gaagacgtgg
    68761 acgagacgcc cggatccctg cgatcgccgg caattgcgca caggctcgag cgggagcagg
    68821 tcctcccggg aatgccggag gaggtcttcc cgttcttcgc cgacgcccgg aacctcgagg
    68881 cgctcacccc tcccttcctc gggttccgga tccttacacc cctgccgatc gagatgcggg
    68941 aagggacgct catcgattac tccctctctc tcttcggctt ccccgtgcgc tggaagaccc
    69001 ggatctccgt ctgggagccg ggcgtgcggt tcgtcgacga gcagctttcc ggtccctacg
    69061 ccctctggcg ccatacgcac tccttcgaac ggcggggggc cgggaccctc atgcgcgacg
    69121 tcgtcgagta ccgcgagccg ctcggtccgc tcgggacgct cgcccaccgc ctcttcgtcg
    69181 cgaggaccgt cgagaggatc ttcgactacc gccgcgaggc ggtcgtcggg ctcctccaga
    69241 gggtcgcgtg agcgcgccgg ccgccgacct cgagctcttc tacgacggcg cgtgccccct
    69301 ctgcgcccgc gaggcgcgcg cgctccggcg gctcgaccgg cgcgggcgcc tgaagctcac
    69361 ggacctcgcc gccccgggct tcgacccggc cgcagcggga gtcccgatgg agaggctcgt
    69421 ggaacggatc cacggccggc tgcccgacgg gacgaccgtc gagggcgtcg aggtcttccg
    69481 ccgggcctac accgcgatcg gattcggccc gctcgtcgct ctgacgcgcc tcccgggcgt
    69541 ctcgggcctg ctcgacctcg cctacgcctg gttcgcccgg aaccgactgc ggctgaccgg
    69601 gcgctgcgag gccgacgcgt gcggccgggg cgtgcctacg gcgtcgaggg gacagcgccg
    69661 acgtcgatcg acggggtccg ccacgtgacc gggtcgacga cgatcttccc ctcgtagttc
    69721 aggacgtcga gcctctccgg gacgaccttc acgagaacga ggttcttcag ccccgggaag
    69781 gactggtccc agtaggcgcg cttcctcttc aggatctcgg ccctgtcgtc gacgaggacc
    69841 gcgcgcccgc tgagggagac gtagccgctc gccttcgcgt ggtcggcgta gtagagcgtc
    69901 acgcgcgggt ccgagcgcat ctcggcgacc tttcggctcc ggtcgttcgt cgcgaaccag
    69961 accgtcaggt cctcctcggg cgggaacggg ttcatggtcc ggaccgaggg gcggcccgac
    70021 gcgtcgagcg tgatcagggc gcagtaggtc tgcgaggtca tcaccttccg ggcggcggcg
    70081 acgagctcgt cgcgggtcgg tggggtcgcg ggcttcgcgg ccttctcgtc cgcgagggag
    70141 acgccgctgg cgaggagggc ggccagggcg aggatcggga ggcggagcgt ccgccgggcc
    70201 ttcgaggaca tcgcgagtct ccttccggga cggagccgct cgggatcgcc ccggttcgtc
    70261 gagggcggcc cgggctcgtg aggcccgggc tgcggggttc acgcggaagc ggtctcccgg
    70321 gactccccgt cgggcgtccc ccggaccgcg gagaagccga gcgccacggc gacgacgacg
    70381 gcgcagccga gcgcgttcag ccagaggaac gagacgtcgg tgaggagcca ggcgccgacg
    70441 accgcggcct cgcccgcgag ggcgccccag aacgcggcgc cccccttcac cttcgggagg
    70501 tagaaggcgg tcaggaagac gccgaggatc gtcccgtaga agaaggagcc gaggatgttc
    70561 accgcctcga cgagcgtccc gaggcggccc gcgtactggg cgaagacgat cgcgaaggcg
    70621 ccccaggcga tcgtcagccc cttcgagatc gtcacgaggt tctcctccgc gacgtccttc
    70681 ccgaagtagc ggcggtagac gtcgacgacg gaggtcgtcg agagggcgtt cagctcggcg
    70741 gccgtcgacg acatcgacgc cgcgaagatc gcggccagga cgaggccgac gacgcccgtc
    70801 gggaggacct ccaggacgaa gcggaggaag acgtagttcg tgtccttccc ctcccctttc
    70861 gggaccgcct ccttcacgac ggccgccgcc ttcgccttca cggcctcgga gcgggcgtgg
    70921 gccgcccgga gcgattcctt ggccgccacg agggccgtct cgtcgcccga cttccccgcc
    70981 gcgacgaggc ggagcgcctc ggtccggcgc tcctcgaacg cggcggcgtg ggccgtctcg
    71041 agagcggcca tttcggcccc gcgcgcctcg gcggccttct tcgcctcgac cgggttgaac
    71101 cagagcggcg gggcgacgaa ctggtagaag gcgaagacca tcgcgccgac gaagaggatc
    71161 gcgaactgca tcggcacctt cacgaggccg ttcgcgagga ggccgaggcg gctctgcgca
    71221 acggagcgcc cggtgaggta gcgctggacc tgcgactggt cggtgccgaa gtaggagagc
    71281 gcgaggaacg cgccgccgat cagccccgac cagaggttgt agcggtcgcc gaggtcgaac
    71341 gtcgtctcga cggcgttcat ccgcccgagc gcccccgaca ccaggaccgc gtcgccgaag
    71401 ccgaccccct cggggaggac ccggaggagg gccgcgaggg cgaagcccat cgcgaggagg
    71461 atgaccacca tctgccacgt ctgcgtctcg ttgaccgcct tcgctccccc ggtcgccgtg
    71521 tagacgacga ccaggccgcc gatgaggagc gtcgtcacct ggatgctcca gccgaggagg
    71581 accgagagga cgagcgccgg agcgtagatc gtcaggccgc aggcgaggcc gcgctggagg
    71641 aggaagagcg acgccgtcag cgtccgcgtc ttcgcgtcga atcgcccctc gaggtactcg
    71701 taggccgtgt agaccttcag ccggtggaag agcgggacga cggtgacgca gaggaccacc
    71761 atcgcgatcg gcagcccgaa gtagaactgg acgaagcgca tcccgtcggc gaaggcctgg
    71821 ccgggcgtcg agaggaacgt gatcgcgctc gcctgcgtcg ccatgatcga gagggcgacg
    71881 gtccaccagg cctggctccg gcccgcgagg aggtacccct cgagcccctt ctgcttccgg
    71941 ctccgccacg tcccgtagcc gacgatgaag aggagcgacg cgccgaggac ggcccagtcg
    72001 aggcccttca ccggaacgac tccgcgaaga gccagaagag gaggacctcc agcgcgaggg
    72061 cacccaggac gaccgcgtag aggacacccc agcgcttccc gaccggcggc ggctcgtcgg
    72121 cgacgggccg ttcgtcagcc acgcggcgtc ccgcccgaga cgaggttcgc gaagaggcgg
    72181 atcgcgcccg gcacgccggc cggaagctgc cggaagaacg agaggccggt gtagacgtag
    72241 gtccccttgc cgtgcctcgc ggcgaggagc gcgcccttcg attccttctc tccggggtcg
    72301 gccatcccga ggagaggcac gtagcgctcg tcccaggtcg ccgggaagta gaggccgcgc
    72361 tcctggaccc agccttcgaa gtcggccggg ccgatccggt tcggcgtcgt caggagcgga
    72421 tgctccggcg cgaggagcgt caccttcgac tcctcgtccg tgacccggtc ccggcccagc
    72481 ttcagcggga acgggccgag ccggtcggtc acctgctcga agctggtgtt gtactggacg
    72541 acgagcgtcc cgcccgcctc cacccagtcg aagagccgcg gcatcgcgtc cttcagctcg
    72601 ctgcgcgtgt tcgcggctcg aatgccgagg acgacggcgt cgaagcgggc gaggtctccg
    72661 cggaggaggt cgtcggccga caggagcgtg acggcgtatc ccatctggcg gaggacgccg
    72721 gggacctcgt cgccgggccc ggagacgtag ccgacgcgcg accggccgcg cgcgagatcg
    72781 acgtggaccg ctcgcgcctc ggcgcgctcg aagtacgtca gcggcggcag gtgcgggtac
    72841 tcgatccggc gcttcgtcag gctccaggag tcgccccccg tcgccgcctc cgcgccgatc
    72901 gtcgcgacgg cgggcgtctc cggggagcgg agcgtgaact cgaacgtcgc gctcgcgccc
    72961 ggcgcgaggg cgaatggccg cacggcgggc tcgacccgcc acccggccgg gaccgagagg
    73021 cggacggcgc cgtcgacctt cggcggcggc gcctcgcccg cctggccgtc cgccccggtg
    73081 gcggacgcga cgatcgacgg cgtgagcggc ggcggcccgg agcggtcctt cgggacctcg
    73141 gcggcccgga ccgtcacctt cacgacccga ggcgcgtggt ccggaaggac gaggacctcc
    73201 tcctcgatct cgacggagac gggaggctcg acgacgaacg ggcggatctc ctcccccttc
    73261 accgggtcgg tcctccggaa gaagaccggc tcctcgtacg tcagtgtctc ccccgccatg
    73321 acgacgtcga aggagacgcg gagcgaggcg ccgtcggggc ggatcgcgtc cttcggctcg
    73381 ggaacggcgt agaggccgcg cgcggggcgc gcccggagcc agtgcggctg cgtcgcctcc
    73441 gcgtcgacgg gaacggggac ggacgccgag cccgacacgg gctcgttcag cttcagctcg
    73501 gcgccggagg ggagcccttc tcccgtgccc cacgggacga cgacgcgggc gagggtcgca
    73561 ggggcgccgg cccggacgag ggccgcgacg ttcaggcgga aggcgctccc cggcgtcgcc
    73621 gaggggctcg ccgcgatcgc ctcgagccag aggccggaag ccgatcggat caccgcgagg
    73681 agctcgcggc gcttgacctc gacccacggg tcggcgccga cctcgcgcag cgcggcgtgc
    73741 gcctcgagga gcttcggcag gacccgcgcc ggctctcgcg cgtcgaagag ccgctcggcc
    73801 tcctcgagga ggcggccgac gcgcgtgccg cccggcacgc gcccccacgt cgtgtcgatc
    73861 ccctcgaacg ggtcggacgt caccgcgtcc ccctcgagga ggacgagggc gttcgcgagc
    73921 cgcccgcgcc gctccgacga gccgaagccc tggctcttgt gctggcttcg gctttcggcc
    73981 gcggtctccg tgtacgagcg cccgagcgcc gggctgtagt cgccgatctc gtacgggatc
    74041 gagccctcga tcggcttgcc gtcccgcctc cagccgttcc agaagaggcg ccggggccgc
    74101 cacggggaga gcccctcctc gatctgctcc gggaagcgcg tcgcgtcgcc cgcggcgcgg
    74161 aacgcctccc gggcgaggat cgccgaggcg gtgtggtgac cgtggccgcc ttcgccgttc
    74221 tcggggaagc gcgcgaggac gacgtcgggg cggaacgtgc ggatcgcgcg gacgacgtcg
    74281 cggaggacct cctcccgtcc ccagacgcgg aacgtctcgt cggccgtctt cgagtagccg
    74341 aagtcgatcg cccgcgtgaa catctgctcg ccgccgtcga tccgccgcgc cgcgaggagc
    74401 tcgtgcgtcc tcacgaggcc gagcgccggt ccgagctcgg cgccgatcag gttctggccg
    74461 ccgtcgccgc gcgtgacgga gaggtaggcc gtccgcatct ggcgcccttc cgcgaacgcc
    74521 gcgatcatcg ccgtgttctc gtcgtcgggg tgcgccgcga cgtagaggac gctcccgagg
    74581 acgttcagct tctggagcct caggaggagc tcccccgcgg gagcgggccg ctcgggcccg
    74641 aagggcacag cggccgccgt ggaggcggcg gcgacggcgg cgagggcgag aaaggcgacg
    74701 gcgagcggcg cgccggggcg cgcggaacgt ctggtcatgc ggttctccgg cgcgcgatta
    74761 gacctgcggc acgctcccct gtctacgcgg agggcgcccg cggggtttcc cgcgcgttca
    74821 ggccctcttc cccgtcagga tgacgacgga tcgcggcccc acctcgaccc ggagcgggtc
    74881 ggccacgagc ggctcggtcc cctcctcgaa gacgtccgcc ggcggctcgc ggtacgtgtc
    74941 ggcgacgaca tgccagcggc gcccttcgga ggcgggaggg agctcgatcc ggtgcgcctc
    75001 gtgatgcgcg ttcgcgacga cgaagacgtc gtccccctgc gcggggtcct tcagctgcat
    75061 gccgagacag gtcgagcccg gcgaccagtc ggggcggaac gggacgtggc cgtgccaggt
    75121 gacgggcgcc gagccggggc cttccgggac gaagctccgg cgccggagaa gcgggctccc
    75181 cttccggaag cggatcagcg tcctcacgaa cctcacgagg tctgcgttct cctcgacgag
    75241 gcgccagtcg agccaggaga cctcgttgtc ctggcagtag gcgttgttgt tgccgccctg
    75301 cgtccgaccg gcctcgtccc ccgcgaggat catcggcacg ccctgcgaga cgaggaggag
    75361 ggcgaacgcg ttccgcgcca tccggcggcg gagccgatcc acctccggcg gcccgccggg
    75421 cccctcgtgg ccgcagttcc aggagttgcc atcgtcgccg ccgtcgcggt tcccttcgcc
    75481 gttcgcctcg ttgtacttgt gcgagtagcg gaagagatcg gccagcggga atccgtcgtg
    75541 gctcgtgacg aagttgatcg aatggtgcgg ggcccgcccg cccccctggt agaggtccgc
    75601 gctccccgac aggcgagtcg ccagcagcgg gaccatcccg ccgtctccgc ggaggaaccg
    75661 gcggaggtcg tcgcgatagc ggccgttcca ttcggcccag cgtccccagt tcgggaagtg
    75721 tccgacctgg tagagcccgg cggcgtccca cgcttccgcg atcagcttcg tgttcgcgag
    75781 gaccgggtcg gccgcgatcc gctcgagcag cggcgggttc gtcaggaccg agccgtcacg
    75841 tcctcgcccg aggatcgagg ccaggtcgaa gcggaacccg tcgacgtgca tctccgtgac
    75901 ccaccaggcg agcgagt
//
LOCUS       DAOONL010000005        73093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_203_length_73093_cov_18.327911, whole genome shotgun sequence.
ACCESSION   DAOONL010000005 DAOONL010000000
VERSION     DAOONL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 73093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73093
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_73093_cov_18.327911"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLP50_01565"
     CDS             <1..473
                     /locus_tag="PLP50_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50263.1"
                     /translation="RREEREGLMPDTKKLLDVVTSAKPGVRFGRVSALLDDAVLVSCG
                     EGSELVACDLLDLEAGRSDALAEGERVLVVVPEGGEGRGVVLGRVDSPAAIAARPKEA
                     PDELLLEAKKGLTLRVGEGSITLREDGKVLIRGRDLVSHARRLNRIRGGSVSIN"
     gene            484..1707
                     /locus_tag="PLP50_01570"
     CDS             484..1707
                     /locus_tag="PLP50_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50264.1"
                     /translation="MISSKVVLLRDVLEEHLEELPFLWQLRRDAVRSPERTRADVDRL
                     DARIRAHANGLLVARGELGELLGPSLASDEACAAFAAGFVLLRTGEEGARAVLDAFRA
                     AGPGGREGLAEALAFGEARYAREELAALAGSDDPSLAVAAATALALGRGAAPPDVTRL
                     LGESDPAVRAAAWRLVPYQARAPRPELLRKGLDDPDPGVAREAAVAAAWFRSPLALEA
                     SRERAAGARDDAGEWLRLLAVLGEDGDVAAVLAGAAKASAAGRGAAGPRALGALGHPR
                     AVELLLEAMGDEDPARAAAAGAAFRKVTGFDAAGETRVALRPDGPPPDDFDEEFLDEV
                     TLPDAQQARAFWASARGKLAGGKRWCYGVDVAAGVPAALPASFDMESCFEIRLRGRFT
                     GTFTGDAGELLRMAP"
     gene            complement(1723..3279)
                     /locus_tag="PLP50_01575"
     CDS             complement(1723..3279)
                     /locus_tag="PLP50_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50265.1"
                     /translation="MSLSGVPARGVPLGWRLGLTTAVIVTVVLGIVTAFVQKREIDRG
                     REERMRFLTMTLAPLAADVGDASDLAAIQDRLTRFQAAHAAGGFGDVRVLLFDAEGRV
                     FATPLTGPAPLPSSDATTARVPVRSRLIPGGSGSLQVSQDEARFRSMSRRLWQHWLLT
                     LAVGAGCILVSLYLAYFFLIERPLRGLLEGVRQMEQGYWSGLEIPRGAWEMRWLAYRF
                     RNLGTQLEETVRRLVEAERRTIAGLRAAAPAGPPARAAGDGAPAGASLQEVAFRKRIL
                     RRYLVARCRYLETRQSSDPAARAVAHEVWERDVHEAQKLGESALKSRLEDAAFRVLEP
                     DAFADVQRSAAGLTSSRRKWIRDREAELKAALTEAGVKPRALQHRVKHVAGIWRKMKS
                     KGLAIEQIHDIFAFRILLETEEECYLALSALHHRFEPLLLRFKDYIAEPKENGYQSLH
                     TCIRAPGGLVYEVQIRTVAMHEAAEGGGAAHWKYKKESVEMDGGEDEQLPQGVLVSLK
                     DALRRRGIGS"
     gene            complement(3416..4303)
                     /locus_tag="PLP50_01580"
     CDS             complement(3416..4303)
                     /locus_tag="PLP50_01580"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPA50266.1"
                     /translation="MEGIIPPLPTPFREDGGLDLGLLVELVTQLAGTGLAGFLALGSN
                     GEAVHLDDDEAERVVSTVRAAAPGRFLLAGSGRLSTRATIEATKRAAGAGADAVLVVT
                     PHFFRGAMSPEALAAHFEAVADVSPVPVLLYNVPANTGLDVDAATVARIARHPNVAGV
                     KDSSGDVGKLAELVRLAPGGKRFDVFSGNYGSALPGYAVGTTGSILAAANVAPAECVA
                     MRQAFLSGRLDEARALHLRLLPMARAVTSRFGVPGLKAALEILGRPAGAPRRPLLPLG
                     AAPREEILAILEQAGLRAS"
     gene            4404..4796
                     /locus_tag="PLP50_01585"
     CDS             4404..4796
                     /locus_tag="PLP50_01585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPA50267.1"
                     /translation="MNVVETPQAPRPAGHYAQAVVHGGLVFVSGQLPHDPSRPGVPPG
                     DVEEQTERALRNVEAILVAAGSGLDRLLSVTVYVADVSLWPRVNAAYARVLGAHRPAR
                     AVVPVKELHHGYLVEVQAVAAVREQTRG"
     gene            4793..5665
                     /locus_tag="PLP50_01590"
     CDS             4793..5665
                     /locus_tag="PLP50_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPA50268.1"
                     /translation="MTTPSPVLPWVAYGVCAVVWGSTYFGIALALESFPPNGMVAVRF
                     SVAALLCLGLGRLRKEPLPSRRELPHLALVGLLLLGICNALVVWSETRLGSGVAAVLA
                     ALTPAWFGVFTAREEPLGARGWTGTALGLAGVTLLAAPWSQKGIDLGGVGALLLASAL
                     WALGTLHHRRHVTGGGALTNSGLEMLAAAVYGVLLAPFTGGFTSGPVTTKALLAVGYL
                     ALFGSCVAYTAHLYVSRVWSPVRAGSYAYLNPVIAVVLGTAILGEAFDARMALGMAVI
                     LAGVALVQYRSRAA"
     gene            5812..10755
                     /locus_tag="PLP50_01595"
     CDS             5812..10755
                     /locus_tag="PLP50_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA50269.1"
                     /translation="MNAPASPGARPSAAAARGRIPRAVRWRGLLLAGSLLLAPSLLAV
                     GPTTDPRQWMVELEGEPAVEVWIRGRAEGSRSALAASAASSRVAELRVAQARVEELLT
                     APAIGARVQYRVTRAFNGLAVVADPSRVEAIRALPGVKSVKPLVLHAPANATSVPHLG
                     LPAGVWQAFANAGDDVKVGIIDSGLDYQHALFGGSGRESDYKANDRTKVDDGFFPTAR
                     VVGGWDFAGDAYGGGGAATPDPDPMDCGGHGTHVAGSLGGSGVRADGTAFAGPFDGSV
                     PFGSMRIGPGVAPRAKLYALRVFGCSGSTGLTTQAVDWAMDPNDDGDFSDHLDVLNLS
                     LGSEFGSKDDSTAIAVENAARAGIVVVCSAGNAGDTYFVTGSPAAADSAISVAASVDS
                     GVTASFVAVLAPSTLAGSMAAGTASFGSTPAANGTIGPLVAAVPARVCSAVTNGAELA
                     GKIALVDRGDCSFDGKVKRAQDAGAIGVVIASNEDAVIGMAVADPAVADAVTIPSVLV
                     SSSNGNRLKAALAEGVTVALYSGSDTLGSFSSRGPRLDGSLAGPKPDIAAPGVSITSA
                     ATGIVAGVAGPELNAGNLAVSMSGTSMAAPHLTGVMALLRKQRPGWTPAELKAAVMNT
                     AGFDVSLLPPAGSAPQYGPARVGAGRVDPARALATEVIVFDDERPSVVSLSAFLQGST
                     PTTVTRRLRILNKGSVAATLTPGWAAPATIPGVRVDVPAAPITVPPGGSATFDVSVVL
                     ADPSAVTNAPDPTLELRQPSFVSLSNRFWMTEASGLVTLSGAPAGTLRVPLQVVVRGQ
                     SAMRAASRALGAPAAGGPFTIPLAGSGLKTGPLPPVDAVSTVSAFELHLDSPRSSPEG
                     SARAVADLAFVGAATSALAGRLDSGATVWFGIATWAEWLSPAEVRFEIEVDRNGDGIV
                     DATVETMATGDVANPSDPSQTVDPTDVWVARTAPALGAGAASYRYLNVDPASRGTHLF
                     ASNVVVLPASAGADGLGLTAGNARFRYRVKASTWRSGTVEETSWLTFDAEKPGYSVLA
                     ADGTPFSDDLPGSALPARFDPAAAQANGALGVLLLHHHNAYGARAEVLSGRNSPPAVT
                     ILEPAPGAAFDAAAPVRFRASGTDADTGDTLGFSWSLGDGRTVSGADVTSAFGSAGPR
                     VVTVTATDGAGATASAQVTIEVRAPQAPSGISKLVPVVLDVKGVGGAPFTTELTLVSR
                     SATAARALLAYTASAGSGSGWAGVDLGPGETKVLPDAIEFLRAQGLAIPDDGSNQIGT
                     LRVTLPGATEPAALFVGGRTSTPGEGGAFGLFYADAVASAGEAIVAGLQQNSAMRSNV
                     TVVNAGPEPVKLRVRLEGASGEDLGVLDEPTLPAWGWKQYDQPLKGRAASGRARVSRL
                     SGSAPFSAYGVLNDAGTSDGSYVPPLLPGAPGAGDRLIPVVLDVKGLGVNRYATEVTL
                     TNLGGAPLATTLVYAAALGGGSGEAPVALAGGEQKVIPDVIAYLRGAGLAIPAGGANV
                     AGSLLVRVPSGTSPDLLAAGARVSTRSSVRDGAFGVFYPGLALSEAANGPTWVHGLQQ
                     DAKTRSNVALVNFGDAGNVTLRLTFHGANGLALADPEVHTLAPGAWLQRNEPLLSRGA
                     AAGSVKVERISGTSRFVAYGVLNDAATSDGSYLPMAR"
     gene            complement(10814..13057)
                     /locus_tag="PLP50_01600"
     CDS             complement(10814..13057)
                     /locus_tag="PLP50_01600"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HPA50270.1"
                     /translation="MHERAARPGDGASLFRSPEYDALPAVDRAAVDALVSTLRVPAGT
                     TVFRRGEPGDAMYVVADGRFRVTGTTPAGAEVTLGEIGPGEWTGEMAILTGQPRSATL
                     TAMEDGALVRLPREGWDELARRYPAVASRLTEAIGPRLRRAQLLRVWEELFGITEPEA
                     FRDLEKAVEWRRVAAGELLVRQGDPSDAMYVVVTGRFRTTVRGEDGVETTVRETGAFS
                     TVGELGLLADQPRSASVVALRDGEVLRLGRDAFRGFALKRPEALLRVASIVAGRQASA
                     VAPKGRASAPVPDGLTIAVLPLGPTAGASAVARTLASRLEALGATLLLDAAGLDARHG
                     RPGVAESPLAGPLGLAVDVSLRELEDRTRHLVLDAGSGGEAWRERCLRRADRVLLVAD
                     ATADPAPGAVDARLAAMGLPARKELVLLHAGETTEPRGTAAWLAGRDLADHHHLRPGN
                     ARDEVRLARRLAGRGVALVLGGGGARGVAHVGVIRALEEAGVEIDLVAGTSMGAVVGA
                     SFALHGASGPLLALAETFASPKRIYDRTLPLVSLMSGGKVLRVMKALYGDRAIEDLWT
                     PFFCVSTNLTRARVDVERSGPLWRAVRKSMSIPGVFPPVIEEGDVVVDGGVVDNFPVE
                     RMASRLDCGTVIGVNVAPAVDKVKPYRFGPELSGWKVLRGRVLPFARKTRAPSLVGSL
                     LRTQEINSVMALRSGLRFVDLLVEPPVDRFRLNEYGRWAELVEAGYGAAKEALPAFLA
                     SRRAGEA"
     gene            13407..13991
                     /locus_tag="PLP50_01605"
     CDS             13407..13991
                     /locus_tag="PLP50_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50271.1"
                     /translation="MRIGKRITTLALTAFVTAALSGCGGAKESPEPAGEPAAAAPAEA
                     PAASGAAKIHLPTGWAMQDAISAADVGAITGQTMTYFPDAGSKAQEGRPSAGYNVPGK
                     DFSVIRFAAVVGGGDAEFESTKGFAAPGSVVEVAGVGDKAYMCDFVTGATVIVVQKGP
                     DVLRVDWQPKTYAEHDKVELGKKLAGKLIENLYK"
     gene            complement(14082..14585)
                     /locus_tag="PLP50_01610"
     CDS             complement(14082..14585)
                     /locus_tag="PLP50_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50272.1"
                     /translation="MIRRPCLALLATVLASVVVAPGARAADLTLHGLKAAPPIPFSAP
                     EEAGLDALRVVHPADAKAGGEKLSLTAVAFPKDSGMSDAELLDYVKTTFLATSAAGKP
                     VERNLLGRKVTGQALEKKIPAPSLAEVYVATKKGGEKVVLAFVLAPGFSEEAEAAIAA
                     IAATLRE"
     gene            complement(14608..15204)
                     /locus_tag="PLP50_01615"
     CDS             complement(14608..15204)
                     /locus_tag="PLP50_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50273.1"
                     /translation="MRPRRAVLLLVASLAAAPLLAQTSDDRGFDPTGVWYGQQGPLVL
                     VRAGDTLSFSYSGVFGATAHICDGIGVAGFVGDGTWEYVDDEGTVTFTTKGGTVSMEA
                     TKGIASFCGANWPGATFTKEGWKPAFSCTVTTPKARFLTVAPSPEPRKGFVVKGDVVE
                     AVGLVNESTPAWLLVRYVGKKQTTAGLLERDALECREE"
     gene            15289..16281
                     /locus_tag="PLP50_01620"
     CDS             15289..16281
                     /locus_tag="PLP50_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPA50274.1"
                     /translation="MRRALPVVFAALLFPATPAGGAEPDRRIVSAAAVALREAPGVSR
                     RVVARLPLGTLVVEVARTGGVDTIGGERAPWLEVATSSGEQGWLFGALSLPYAEAERD
                     ALALEIAGDRLKRAGDPFEAFAELHAFLARAAGEAATPEARARIELATLRALDRACGA
                     IGFRRRREEKVLAWVEAQARDLSWNEPAARWIVVADRFWELHDRSASLSVADEIAWEA
                     ARTQLPGECEGDVPCRFSVLGRTDVRYLERHPRGAHAGEALANVATALEEAVGDPRGA
                     ALFAGSPREDLERTRNDLEKLKALLARIDDPRAGAARSAADELLARAAVAGGKG"
     gene            16278..16736
                     /locus_tag="PLP50_01625"
     CDS             16278..16736
                     /locus_tag="PLP50_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50275.1"
                     /translation="MSGPPTGAGGMPSAAASAERNPARIVAYVTSAVGALTGPVSFLA
                     FPGFGVWTTEDLPGAGVVALAALVMASPFAAFLLAARLSKSRTGAAVVAALALVAVLF
                     GALYYVWAAVEKNVLILLASVGVPAVQWLMALPAVVAALVLRPKRWSAPR"
     gene            complement(16761..18923)
                     /locus_tag="PLP50_01630"
     CDS             complement(16761..18923)
                     /locus_tag="PLP50_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HPA50276.1"
                     /translation="MGRIVQSVAMSYVRGCLVSGVVTIYVFTILVGFLLLPLGRMHRT
                     PGADPKEIFLPLGIGAFFLLVLPALVAYLVTRWRNAAFDRMFGALGLAGFPYAVQYRR
                     YDGTYGGRRFQGFFSRGPRLALEADVAVWTRFGITAGSGDTQLLAALAGEKPIRFAVP
                     AFDGLDVFGEEEAWVRRLLAEPGVPTLLARLLRYQGPFARRQLVLRPGALNVTFHLST
                     AFMSWIPSTAQVKDWADALVALADIAERVLPPEVPVAPTRLEQQVDSVRRMGFAVNPG
                     ALALGAMLATSLAIAAIAGIIVVAQKSCRPTRGPGSVAEVRDVLHHVYATAQSLQVAE
                     LPAASMLDLYGNAGSLGTVESEDATSLRAIAKVPNGARIVVTKSPGMAAAREVEWLFD
                     PPLSAKVSVVEQQLGKLELSPAPGEPKAVLGLATPRKADGARWPVRVRVRFEGKAGGP
                     ITRISAYRDDPAVPAPSASAGEAPRASVSTPSSAGETAAAPPAAASSGLLPQCPSLFS
                     NIGEMAVGESRSCVCTRDYGIGIRGNGRYDPSSSLCEAARHAGVLTRPGDVVTFWRQP
                     DCPRLWGSAANGVVSKNFASPTATFSFTAEPPPCPPPPSTAADLKPCPGLFSKELEAM
                     PEGSSLDCTCAYDKYGKGSLWGTRVYFLGSNVCDAAQHAGMVDRKGSAVTVFLGGECD
                     RFWGSRSFGKVSDSRRKPGRSMAFQQPYPECPGPTDPW"
     gene            19036..20277
                     /locus_tag="PLP50_01635"
     CDS             19036..20277
                     /locus_tag="PLP50_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPA50277.1"
                     /translation="MTDFTFLCNECGARYGEDRSLYVCPACARLQAPGSFTRGVLRVE
                     LAEQSLPRRWPDAPLASPEGLAPFLPVRGPDSFFSFAAGGTPLLPVPRLREALRMPRL
                     FLKDDTRNPSGSTKDRASALVVAKAIEYGYETVAAASTGNAATALSAAGAASGVKTVV
                     FVPASAPPAKLVQMASYGARLVPVAGTYDQAFELSLEACARFGWYNRNTALNPFTVEG
                     KKTAALEIARGLGREAPDAVVVPTGDGVIVAGVAKGFADLVRCGLLPRVPRLVAVQPE
                     RSGAIARALRSGAAEITPEPDAGSVADSLTVAAPRNAVMALADVRASGGCGVLVSEAA
                     ILDAIPRLAAATSVFAEPAAAIALAGLELALAEGKVGREERVVLLVTGTGLKDVPAAA
                     RRVKVPEPLEPTIAAVEALLR"
     gene            complement(20292..20768)
                     /locus_tag="PLP50_01640"
     CDS             complement(20292..20768)
                     /locus_tag="PLP50_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50278.1"
                     /translation="MTRCPAPLAIALALATLFSAAACAGSEARDAAASPAAPARPDEG
                     GPARMRVVRNEKAVETVAGERLDLVVTRPVGIPEQLQVEWPADPAIDGDAVRFVRRRV
                     EAPPPEDDGGVTTLHYELEAVRPGAARVTLAPKPASRDAAPPPVVIDVTVRDAAPR"
     gene            20992..21357
                     /locus_tag="PLP50_01645"
     CDS             20992..21357
                     /locus_tag="PLP50_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50279.1"
                     /translation="MHLFSARLLSAAALAVALLVPFAASAQKAPPNDVTKAEFYLKKL
                     EDHARRARGAAFRPGYEDNEALKRIKELKEKYPDDPAVEALFARARTALMGSKGDFVE
                     ITPEMLRYRDQERKIVELF"
     gene            21463..22323
                     /locus_tag="PLP50_01650"
     CDS             21463..22323
                     /locus_tag="PLP50_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50280.1"
                     /translation="MPGKYVVLDGLEYPANQFTDLGREFVFWGTPARGMYYVQIDTPS
                     WRGAYEALKRYRRLVDTGLAEGTPFSVAGRIEGAELAVPEAGRKRFGGAFWGWVVVPE
                     AIWVDGRAFAVADRNAELGGRFAGEERMEEITGPLYSVKSIPADVTPERLVEIFATAI
                     KEKNLKLYLDCIDPKRLQGPRARSLAMYHWDWHQVRFRDFYVHTTVEKVEKTMVKGYD
                     AGNDLESFFLTDEQKAQVKKISEPSVERATVTTKAWDERGRQYGSPKPHELIRRGGGR
                     WYVYDFAAQF"
     gene            22334..23650
                     /locus_tag="PLP50_01655"
     CDS             22334..23650
                     /locus_tag="PLP50_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50281.1"
                     /translation="MKTTLSQNLPVRLTALLGAAFLLGTNGLAQVPDSLHPDVRNPFQ
                     EEKKDTREKTYVQKTALRWELSRRHSEHVIEEYLPKFEAIVKRKPGSSDVTEWRSLGG
                     QLFVSWEESRKHEKTGTRPSDQDEWEKQHNSFLGGISRRPTLLDWREGVAISAAAFQK
                     EADEAPGRFEEIRRNMQATDPVWAKAIEIAAGGSGTAWNREKLKEAQDAYANLKRMRD
                     QVDSTYSAITWAYAYAAKDNAEKPRTRLTTDEQAALKSWKTAVSTYPLVQKIYTQPVQ
                     EWAKLFEAAFDAEVKAWEAMQKAFEPVKSGSIRASSPYKDCSWDKRVAECVQKTLDDL
                     ASAIAGAARKIEGADAKVSEDKALTEKEYRRLVELGNLVPPGEWRKLELAEARARTDE
                     ELADAERKITEFRATAKKVADESAKIKAEHKARRKALGIPPEDWAD"
     gene            23691..24170
                     /locus_tag="PLP50_01660"
     CDS             23691..24170
                     /locus_tag="PLP50_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50282.1"
                     /translation="MSRRSLFWLVVVAAVAFWLWKPGEVSNEGSAIRTSSAGTSVGAP
                     SATGEACLYAAEEASRLTQEAADVLLRAPIDTAVFSAASERASAAIASAEGMCGGGSS
                     AGEQRAMEEARGALSEMRGLLSDFAGTLSGSGSASDAPRRRETIDARLVAARAALRG"
     gene            24266..24625
                     /locus_tag="PLP50_01665"
     CDS             24266..24625
                     /locus_tag="PLP50_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HPA50283.1"
                     /translation="MSSHDAHYGGNLVDGARMLGLFGDVATELLIRHDGDEGLFRAYD
                     SVDFLAPVYAGDYIEAEGTITAAGNTSRKMAFEARKVIRSAPEVNDSAAEILEPPVVV
                     CRATGTCVTPKEKQRRG"
     gene            complement(24695..25147)
                     /locus_tag="PLP50_01670"
     CDS             complement(24695..25147)
                     /locus_tag="PLP50_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50284.1"
                     /translation="MTPRSAALILAVAAIVAAGPGTAESAPPADGHPAWTVESCFSCH
                     RSDEAAAISPRVGRPCRTLCATCHEPREGHHPVGVRIPRSVPAPLILTAGGTNTCVTC
                     HDTTRPRRDTAPWESRSLFARIALRTKEYPTRYLAVKNDRGQLCRTCH"
     gene            complement(25158..26270)
                     /locus_tag="PLP50_01675"
     CDS             complement(25158..26270)
                     /locus_tag="PLP50_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50285.1"
                     /translation="MLSPRRPAALLGLLLLATTLAPRAGRAQEPAAPEAEGLAGALAE
                     IRDALGLSGFFDVKAADTRTDPNVFSMGDFELDLERDLGRHVQVAAALVVNDGGAELA
                     VGFVDVHVVGAPVAPRGRLPVEKGFHAQLGRFDVPFGGDWQYFAAKDRTELSAPLTTE
                     AVLDGGYNDVGLRLLGGTGSLGWTAYWLRGEGEGTAVGGRIALMPFDDVYRLRDRTRV
                     FELGISALHDSDGNGNTETTSLAVDAELRATFGQLRAEWARRDERPVEGRDARLVRSG
                     LHVTAIVEAGAVAGVPLSPYVRWDTAKAEPEAAGGEVGVEAGRTERLTAGLRTLLFDT
                     LTLKLEYQRVLAAPPEAEAEEGFRRDALLAQAVVAF"
     gene            complement(26272..26472)
                     /locus_tag="PLP50_01680"
     CDS             complement(26272..26472)
                     /locus_tag="PLP50_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPA50286.1"
                     /translation="MDTLITVTIVAAAALWAALRLFRRRRPGACGGACSGCPAARSTT
                     GGNACAPVPSGRSLPGWNDGGR"
     gene            complement(26487..28616)
                     /gene="feoB"
                     /locus_tag="PLP50_01685"
     CDS             complement(26487..28616)
                     /gene="feoB"
                     /locus_tag="PLP50_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPA50287.1"
                     /translation="MALTLLAPQSTIPVAARFRIALAGNPNCGKTSLFNALTGTRQHV
                     ANYPGVTVERRTGTFSLGDATVEVVDLPGTYGLSAFSPEERVAEDELATGPDVVVVVA
                     DSMQLQRNLVLLSQVLLAGANPVLCLNMADEARAAGQKLDVPLMEKLLGLPVVETVAR
                     TGEGLDALREAIRRAAAAPLPRHRVVLGEELDGALSAIAPLLPGEVAPRQREWAALRL
                     LLEAGEPGPRVEGPAGREKAAAEARRQRARLEAKHERAISVFVAERTFGFVDGLLRET
                     RTPPPRIDARVLSDRLDDVVAHRWLGLPIFAAVLYGIFWLTFTVGEAPMGWIESGVGL
                     LGSAVSGLWPAGSESVLRSLLVDGVIAGVGGVLVFLPNILLLFLGLALLEDSGYMARA
                     AFLTDRAMHRFGLHGKSFLPMMTGFGCTVPGILATRTLENERDRLTTMLVLPLMSCGA
                     RLPIWMLLVPAFFPPAWRAPALWLIYASGIALALLLALLLRRTILPGEDAPFVMELPP
                     YRIPTLKSVVLKMLDRGGLYLRKAGTVILGISILLWAATSFPKPSSYAVDARLAAGEA
                     IPESDLANARAAEELSVSVAGRAGRLLEPVFAPLGFDWKMVTAMIGAFAAKEVFVAQM
                     GIVYAIGDAEEGAAGLRARLARDYAPLVGISLILFLLISAPCMATIAVTRRESGRWRW
                     AFLQLFGLTAVAWLVSFTVFQVGRLFL"
     gene            complement(28645..29361)
                     /locus_tag="PLP50_01690"
     CDS             complement(28645..29361)
                     /locus_tag="PLP50_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HPA50288.1"
                     /translation="MSEPLEDYLETIYLLVQEHGFARVKEIAKARGVKAATVSIALRK
                     LSEAGFVNYVRREYIGLTERGEEAARRVLTRHRLLTRFFSEVLGMSPHAASEQACSLE
                     HALTDEAMDRMVRFFEFLGTRPSVVAAFGQCPTGSRPGDVEPCGTASPDCARCSLAPK
                     EKAMSIATMKPGQSGVVTQITATGALRQRLLDMGILPETKVDVERSGPGGHPLWIRCQ
                     GARLALRRAEASSVLVRAGA"
     gene            29672..31684
                     /locus_tag="PLP50_01695"
     CDS             29672..31684
                     /locus_tag="PLP50_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPA50289.1"
                     /translation="MSPGWRTALVTTLRERCRVCFACVRECPAKAIRISGRQAEVIPE
                     RCIACGNCVRVCSQGAKQAVRSIAQVEALLASGTPVAACLAPSFPAEFPELPVPRLVG
                     AVRALGFAFVQEVAFGADLVARRYRELLAEPGTRYIATTCPAVVSYVERYHPSLVELL
                     APVASPMVATARVLRRLHGPDLHIVFVGPCIAKKGEDRWPGADPGVDEAITFGELREM
                     WREKGIEPADASPEEFDPPRAGSGAIFPVSRGMLQAAEVPEDLTLGEVVSADGRSAFV
                     DAVKELESGDLDGATRLLELLCCNGCVMGSGMTSTAPLYKRRALVTRAARERLRELDR
                     GRWEGEMSAYADLDLGRSFFARPVTVGAASGEDVAEILRRMGKPSPADELNCGACGYD
                     TCREHAAAVHSGLAESEMCLPFTIDQLRNAVREIAVSHDQLASAQQALLHSERLASMG
                     QLAAGIAHEVNNPLGVVLLYSHLLLEGVPEGSSLRGDLQTVAAEADRCKKIVSGLLDF
                     ARQNKVSLSEVTVDDLVAQSIRGVVLPERVDVEVEHEDPGLVVELDRDQIVQVVANLV
                     RNAAEAMPRGGTVIVRTRAVGSSLVLSVTDGGTGISKENLSRVFEPFFTTKPIGKGTG
                     LGLPVSYGIVKMHRGDIDVVSNADPAAGPTGTVLTVRLPLRGTRGA"
     gene            31687..32847
                     /locus_tag="PLP50_01700"
     CDS             31687..32847
                     /locus_tag="PLP50_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPA50290.1"
                     /translation="MSGTLKILVVDDEPGIRLAVSRVLEGHRTTLPDESTETSFEVAQ
                     AGTGEEALAACTAGAPDILLLDYKLPGLSGLDVLERLGPATAETIVVMITAYATIETA
                     VTATRRGAFDFLAKPFTPAELRTTVSRAATHLALQRRAKRLAEEKRQARYQLLSVLFH
                     ELKSPLAAVGGYLDVLREGFLPPGSAQYDEVLERSRARLEGMRRLVVDLLDLTRLESG
                     QRKREPVELDARDIVLRAVETAQPAAAARRIEIFVDAPEAAPLVADPGELEIVLNNLL
                     SNAVKYNREGGRVDVRLARDAGGVRLVVADTGIGMTAQEVGRLFGEFVRIRNEKTRDI
                     PGSGLGLSIMKKVVELAGGEVSVESEPGKGTTFTVRLPSGALPPVLTAPHVD"
     gene            33080..33505
                     /locus_tag="PLP50_01705"
     CDS             33080..33505
                     /locus_tag="PLP50_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50291.1"
                     /translation="MARILIVDDDKDIVEACRLFLEREGHTVAAAYSRGGGMRAVSDW
                     PPDLLILDIMMDEPDDGIRMAQELRRSGWTTPIVMLTSISRVTGLGYRADPDVLPVDA
                     FLEKPVRPAVLSKTVADLLARAGGREESHAGHGTAPAAR"
     gene            33471..33992
                     /locus_tag="PLP50_01710"
     CDS             33471..33992
                     /locus_tag="PLP50_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPA50292.1"
                     /translation="MLVTERLRLHDDIGAWVAKHGATRAALIPVLQEIQRSYNEISEY
                     AMQEVADRLDIHPVEVYSVVSFYSFLEAKARGRFVIRLCRTVSCDMTGKDRVARQLEN
                     DLGIRFGETTRDGNFSLEWANCLGMCDQGPALLINDQVFTSVTPETVHDLIQACRKSF
                     GPLAVQGQKGRTH"
     gene            33992..35182
                     /locus_tag="PLP50_01715"
     CDS             33992..35182
                     /locus_tag="PLP50_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPA50293.1"
                     /translation="MSAVKKGLVTFAAAVPEEGLKAALARPRSEVIAEVTASKLRGRG
                     GAGFPTGVKWNLAAAATAPAKWVVCNADEGEPGTFKDRVILTEAPDLVLEGMTIAAWA
                     IGAARGILYLRGEYAYLRDSLEKVLARRREKGLLGTGILGSPLSFDVEIRMGSGAYVC
                     GEETALIESLEGHRGEPRNRPPFPVDCGYRSEPTIVNNVETFACAAMVLAKGAEWFRG
                     IGTEVSTGPKLFSVSGDCREPGVWEFPLGITIAGLLAAVGGEGAKAVQVGGASGRCVP
                     AAEFGRTLAFEGVPTGGSIIVFGPRRDMLHVAENFLEFFVDESCGQCTPCREGNAKLL
                     EGVRMLREGRCSWAYLQELQKLGEVMQLASKCGLGQSSPNAFLSIVEHFRPEIMGRTA
                     GGNA"
     gene            35195..37135
                     /locus_tag="PLP50_01720"
     CDS             35195..37135
                     /locus_tag="PLP50_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPA50294.1"
                     /translation="MSSRIERTASISRAPVSQRGHVVPVSADPEAIGGTVSVTIDGIE
                     AKVPLGTTILDAARALGLKIPTLCHHPDLEVAGVCRICVVEVEGQRTLQAACAFPITS
                     PIRVQTHTRKVRQARRRILDLMLSKHVGECYTCFRNLNCELQTLAKEYGVDFFRFGHE
                     AARRHQVDDSSYSVRRDMDKCILCRRCVRTCIDLQEVGVLEAVGRSDGTRIATFLDKP
                     LNDVVCINCGQCINRCPTAALGAMDPTDDVWIDINDPKKHVVIQTAPAPRAAIGECFG
                     LPPGTPLTFEMNTAMKTIGFDRVFDTNFTADLTIIEEGTELLLRLHEALVQKKDVALP
                     QFTSCSPGWVKYLEHFYPEMIPNLSTAKSPQQMFGALIKTFYAQKAGIDPASIVACAL
                     MPCSAKKYECNRPEMCDSGHKDVDYGLVTRELGQMFHEAGIDLPKMPKGAFDDPFGTA
                     TGSGVIFGATGGVMEAAIRTVVELVTGLKIEDVFAHADVLPVRGFDGVRYVELPIPAI
                     GEVPDLLKMNKGLPFSDWKWLEGAVLKVAVCHGTANAKRVVEDIKAGGTFSKCHFIEF
                     MACPGGCLGGGGMPIPTSPEIRAARAKAIYAEDASYEIRKAHENPAVIRLYQEFLTDG
                     PCGEKSHHLLHTHYTPRGKYIA"
     gene            37206..38510
                     /locus_tag="PLP50_01725"
     CDS             37206..38510
                     /locus_tag="PLP50_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPA50295.1"
                     /translation="MSRSGAPPPPFLAPREAKVLDLVNRKVAAGRSLEEVLDFLFRAT
                     RDVCPCDRIGVAFLEEDGQRVVARRAVADYEPVLLGAGWSEDLAATSLGPVLASEMPR
                     VLDDLPAYLSARPSSRASRLLVDEGVRSSLTARLEVEGRVFGFLFRSARRVRAYDARQ
                     AFLHLAVSERLAQAVEKAWLLDRLAEASAGYVEMLGFVSHELKAPLASLVMDGEAVLG
                     GYAGDVDEAARAAVERMTRKASYLGRLVKEYLDLARVEEHDLVARRVPCDFVADVVEP
                     ALDVVRTLLEERSGRLVRDLASGPLPVSVDPELMTVVLVNLLGNAAKYARDGGEVRLS
                     ARCDGESLRVAVRNEGPGFRPEERARLFRRFSRLADPELAGRPGSGVGLYTCRRIVAL
                     HGGRIDAESEPGCWAEFRFEVPASLRTVEAGEERSEPVRSRP"
     gene            38719..39333
                     /locus_tag="PLP50_01730"
     CDS             38719..39333
                     /locus_tag="PLP50_01730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA50296.1"
                     /translation="MAATAEAARRAAFGALAATWDAAKPREAIEAGVARGLDLLGDVR
                     GLRAIDLGAGPGRLEPFLLPRLGGGHAVAVDFAPEMVVRGALEVRDPRVTWLCRDVLE
                     TGLPDACADLVLCFNAWAHFPDGPGVLREARRWLKPGGRILLWHDLGRERLAEVHRRA
                     GHAVSCDLLPPVEALAFAARNAGFAVERAEEDDRSWTLLACSVG"
     gene            complement(39341..40387)
                     /gene="dmeF"
                     /locus_tag="PLP50_01735"
     CDS             complement(39341..40387)
                     /gene="dmeF"
                     /locus_tag="PLP50_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HPA50297.1"
                     /translation="MTPADPASAAHEACATGRRHEHVFGQDRPRPGERGTRIVIALTA
                     VTMLVEIGAGLKFGSMALLADGLHMGSHAAALGISAFAYAWVRRHATDPRFSFGAGKM
                     NALAGFAGAVLLAGFALLMAWESVERLLSPVPVAFSQAIAVAVLGLAVNAVSALVLTR
                     GERPHDGHDGEPSARPADHAHDHGHDHDHNLRSAYLHVLADAVTSLLAIGALLGGALL
                     GWRFLDPLIGIVGAAVITRWSVGLLRESGHVLLDRQAPEPLLSSIRGAIEARPGDRVS
                     DLHVWRISPDGWSAIVSVVSDDPLSPDGYRALLPEGLDLAHVNVEVLPCPHAGEPKRD
                     CGTAELARPPDGVG"
     gene            complement(40384..43209)
                     /locus_tag="PLP50_01740"
     CDS             complement(40384..43209)
                     /locus_tag="PLP50_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HPA50298.1"
                     /translation="MRISTVALAALLAASPTRAIPPNAPAPFTALSLVGRAVLPRDLE
                     YEKTRVGGLSGLAWDAESGRFLAISDDRGRFGPVRVYRIRLDVADARDGRFPFTATAS
                     VEGMTLLTDAKGRPFVPSRIDTEGLALLPGRLLVSTEAIARHGIPAAITEHAPDGRLR
                     RELALPAKLLPAPGRGVRDNLGFEGLAATPDGRYLFAGLENALEQDGAPADVKTPSPA
                     RLLRFDLASGGPPTEFVYVVDPVSPPPLNEGLFRVNGLSDLLPLGPDRLLVLERQFID
                     GTGNAARIWDVSLQGATDVSSLESLSGAEYVPARRTLLLDLAETGVALENFEGLAFGP
                     TLPDGRASLVVASDDNFNPEQESTTFLVFAVDHGPVSVARIQGAGHRSPIEGSRIWDV
                     PGVVTAVERDPRNPGFFLESLRPDDDASTSEGLFVSWSAAASLEPGRAVSVSGRVEET
                     EQPKGLSVTRLSASAVVPLGGPVALPPPARPFARGPLPSVVDDDALARFEPASDALDF
                     WESLEAMRVEVPAGAVVGPSSRRGDFVLRPDGSPEVARTAVGGVLLPERGPSLDRVFV
                     SRPFAGDPAILDVGARVVAPFVGIVDYAWSTYRVRPLVAPATTAAAGGCDAVTSLRGD
                     RRRLSVASFNVENLSAAGPAERFTRIGERIAKAMLGPDVVALEEVQDDSGPAGKGDGV
                     VTSKRTLESLVDAVVAAGGPRYEAVWIDPIEGREGGQPGGNIRVALLLDPARVTLVRR
                     GSAGPLDATAPEGTGKDLRLTLSPGRVAPASEAFSPTDGEGVRRSLAVELLFRGKKLF
                     VVANHLSSKSDDDRAFGSVQPPRAPTVARRLAQAREIRAFAQSLLAVDPKARIILLGD
                     LNDFEHSEAVRALSAPPLENLVERVPAANRYTFNFEGASQVLDHVVVSPALAREAEIE
                     ILHLNSDCSDEKRTSDHDPVVARLRPR"
     gene            complement(43326..44240)
                     /gene="ftcD"
                     /locus_tag="PLP50_01745"
     CDS             complement(43326..44240)
                     /gene="ftcD"
                     /locus_tag="PLP50_01745"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPA50299.1"
                     /translation="MSQIISVVPNICEGRDEAFVAKVQERLETVPGLVVLDVSMDQVR
                     NRTIFSFTGSKEAIFQGGYLLYEEALGKIDMRQHEGEYPRIGAVDVFPFVALRDAPLP
                     TVVDWSVQFAEEVAGRFALPVYLFAESARVKARRDIESIREGEYEGFAAKMNQPEWKP
                     DFGPDVFPPDKGATIIGARLPIVNFKTYLATSSEEAAEWVSHVLSDPATGFSGVHFYP
                     ALDRTRNEALLNITVGNFLATPLYRILEAVKTELRRFGTRVSRVEMVGLVPQQALVAS
                     AEHYLQIFGFSADDVVENRLDAIFFGKD"
     gene            complement(44312..45481)
                     /locus_tag="PLP50_01750"
     CDS             complement(44312..45481)
                     /locus_tag="PLP50_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPA50300.1"
                     /translation="MTGYLAQVVQGSGFANLTAGNVVMVLVGLLFIYLAIAKDYEPLL
                     LVPIGFGILVGNIPMPLSIFNSVSLYMIDPVSHEYVFNSGGNSVLGIIYFGVKAGVFP
                     PLIFLGIGALTDFSALLSNPKSLLLGAAAQIGIFMTFLGALWLGFSPQGAASIGIIGG
                     ADGPTAIFTASRLAPALIGPIAISAYSYMAMVPIIQPPIMKLLTTKEERLIRMKPGRK
                     VSRFEKIAFPIMGLLVTTLLAPGGLPLLGMLFFGNLLKESGVTTRLAKSASSAILDTC
                     TILLGIAVGASTSAGVFLKPQSILIFVLGCVAFGTATAGGVLFAKLMNVFAKEKVNPL
                     IGAAGVSAVPDSARVAHMVGQAEDPGNFLLQHAMGPNVAGVIGSALAAGVFLGLF"
     gene            complement(46047..46538)
                     /locus_tag="PLP50_01755"
     CDS             complement(46047..46538)
                     /locus_tag="PLP50_01755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869046.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPA50301.1"
                     /translation="MTQHVLRIGGREYQAEVKDLTPEHALILVDGKEYEVEILQLGRR
                     VIPVDAARPSAAPTPRPAATSGVRPAAPAPAAAPRPAASARGEGGIVAPMPGLVLSIK
                     AKEGDTVAAGQTLLVMEAMKMENAVTTPYAGTVAKVYVREGDSIGEGDLLVDVARPKM
                     TTL"
     gene            complement(46557..46874)
                     /locus_tag="PLP50_01760"
     CDS             complement(46557..46874)
                     /locus_tag="PLP50_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPA50302.1"
                     /translation="MSQLEALTITALGMAVVFIGLVACIAFIRLFGRLSARIAWGEEG
                     HGHGAPAPAPVPEPVPAAAPAGEPIPAEVLAVISTVLQVERKLYLNRPGSRVTIRRSA
                     PRP"
     gene            complement(46884..48434)
                     /locus_tag="PLP50_01765"
     CDS             complement(46884..48434)
                     /locus_tag="PLP50_01765"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPA50303.1"
                     /translation="MSIEHRILELAHLRQNAYRAGGEKRIQKQHEQGKYTARERIEKL
                     LDEGSFEEFDTFVTHRCTDFGMEKDQPLTDGVITGHGTVNGRLVFVFSQDFTIFGGSL
                     SKAFAEKICKVMDLAVKVGAPVIGLNDSGGARIQEGVDALAGYSDIFLRNVLASGVVP
                     QISLVLGPCAGGAVYSPAITDFVTMTRGTSYMFLTGPKVVKQVTRETVTTEQLGGADV
                     HAGKSGVAHFVAENEDEALALVRRLLGYFPQNYQERAPLLTCEDPIDRPVPELNAVLP
                     DGANQPYDVKDVIRSIVDCGEFLEVHEAWAANLVVGFARFNGRSVGIVANQPKVLAGV
                     LDNAASIKGARFVRFCDAFNIPLVTLEDVPGFMPGTTQEYGGIIRNGAKLLFAFAEAT
                     VPKVTVILRKAYGGAYCVMSSKHLRGDVNYAWPTAEIAVMGPDGAVEIIYKKELDAAE
                     DVAAKAAELKEKYASLFATPYVAARKGYIDDVIKPATTRFRIIKALEMLGEKRDGNPA
                     RRHTNIPL"
     gene            48639..49358
                     /locus_tag="PLP50_01770"
     CDS             48639..49358
                     /locus_tag="PLP50_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50304.1"
                     /translation="MKRKSLTVVLAATLLLLGVPVLATEIDAETEARVPALTAFHEVV
                     YPLWHEAWPNKDVDALVALAPKVDAGVAAIAKAELPGILRDKKAAWESAVARLKAIGA
                     EYAAASTKKDGTALLAAAESLHAQFERMVRIIRPVLKELEAFHATLYVLYHRQLDPFA
                     LEAVTQSVRTLDDKMVPLATAALPERLKAKEEAFLAARGALSAAVDEAVATLGRKDEK
                     EIRAAIELLHTRYQELEKVFD"
     gene            complement(49443..51029)
                     /locus_tag="PLP50_01775"
     CDS             complement(49443..51029)
                     /locus_tag="PLP50_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50305.1"
                     /translation="MKRAIAGGALFTLVTGGAYLLLPGWTTRLVEKLTAPSPAGLLAH
                     LAAGIGLVAVAAGATFAREVLFSRAALEAGERARRALATSFLDVRSGADGERGTTISA
                     FFSEAALLERGLLDGVLAFLPNVVLVIALLGVMTWAAPILAAVLLVAAVPLVLASQVA
                     GAAMPEALEETRRARSDLGDALEDLLVGREELVALGTPPGPLGRLTGAAAALRSAAQC
                     EVFARAAHPLRVTLSSTLAAAVVLSVAWGLHAAGRLDSPSLTTFLVCLGVSSAHVQAA
                     GSSLPHLQVFLGVRRRFRRLLAAAPPVSAMPIGRDVDRIELVGVGFRWESGFRLESVH
                     LSAGPGDLVVVHGPSGSGKSTLLRICAGLLEARSGEVLVPGGGEAPECLRRSLGRSIG
                     WLRQEPHVFRGSLGENLRLAAPDASDEDARQALAASGAPDLVASLPDGLGTLLRPGGV
                     VLSAGQRQRVALARVLLIRPSLLLLDEPTAALDAEAEETVFATLRWLAKRGTGVVVAT
                     HRPHLDAFATKVVTLPSPTS"
     gene            complement(51026..52201)
                     /locus_tag="PLP50_01780"
     CDS             complement(51026..52201)
                     /locus_tag="PLP50_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50306.1"
                     /translation="MRLLLTANGPGELLAWAAPLLARLEARGGFTTEIVLWPCRFASG
                     REPELAATLPTVSSVVPPAEAIRGLLCRRPGASFAPTDVLLHIGGEPALSLLLARRLG
                     CPAVAYHEPDGAIFRLFERSLPVAGPRAVGDLRTDAWAEVGASRERVDENRADGLVLA
                     LFPGSRALLVRHFLPFQLLVASTLSRSLPGLGVLVTLSDLVTAEAFLAALEGSPGILP
                     GSRARLLGTPGPGGTLEVEGLPPVPIVRTGRALREADFGLAPVGSVTGEAAAAGLPLV
                     ALLPTHDPALVPIPGLAGQLRRLPFVGRGLHATAFRTFVARRPLVALPNLRAGRPLVP
                     EVRGDFGTEEVVTALLGLAREIQDGAARARLANGLRSAMGPAGAAGRILAELEAVVA"
     gene            complement(52198..52929)
                     /locus_tag="PLP50_01785"
     CDS             complement(52198..52929)
                     /locus_tag="PLP50_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50307.1"
                     /translation="MATEAAVRPFEARDRDDVVRICEETAFCDRGAAQLPCPGFVAAW
                     FVRPYLDLEPRHAFVAVERGQEERVVGYVVGTPDTARFRRRSVPRALRILAALAVASR
                     EPGPRRLLRWLAGPGRREAPRIPLDRYPAHLHFNVLPLARSRQLAGLLLHTVVASFGE
                     GATCIHGVTTTRRGESALPQRLMALGVADGAQILDETPSTFLPAVLGEQGEFVNRAWG
                     VSIAEVLAVAARLQRPGLGRRSRPR"
     gene            complement(52919..53923)
                     /locus_tag="PLP50_01790"
     CDS             complement(52919..53923)
                     /locus_tag="PLP50_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA50308.1"
                     /translation="MSGAAAPDVSIVVTAHDAAETVSRCLASIDATRAAAPPSEVLLV
                     DDRSEDGTAEAARATGVSGLVVLRNEGLPTAGLTTRQTSLDQGIRAARGGAIYLTDVD
                     GRVPSGWLARADEVSSGRVDVLVGRVRYEGRGLLSALMTCDGVVYQFLCSSLDALGAA
                     SGGVAGNLAFRREAYLAVGGFERLGPSLTEDLLLVRALQRARFRVRFDARSAVTVPAP
                     ASWSSIVARGRRTSRGESGLLGLAIALALVSLAGPAVVAAATGGAALGFLAARWLLGA
                     GVLAFLCLERGELRAAAVAPLFEPFALATAAAVLAARAVSPDVVWGGVRYRKGLPVGD
                     "
     gene            complement(53920..54639)
                     /locus_tag="PLP50_01795"
     CDS             complement(53920..54639)
                     /locus_tag="PLP50_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50309.1"
                     /translation="MTSPKRLLLVEIHDARPAEAETIDRLRSLAAETGAGRPALLVTP
                     EWGGARASRRALARLVTPSDTPVLHGLTHDAPPSAFERLLYGDAMGSEFARLGRQEAR
                     ERIRRGRLLLGEATGRRVEWWCAPRWREPRHVRGLLAELGFEGTLGRRALLLSSGQTH
                     AAPVLSFDHGTRHIVVAANRLVRRLRLGPLLRSARIVRVALHPRDATSPSTVAEVRNL
                     LAALTSGGFVPVDLATIAGGA"
     gene            complement(54636..55751)
                     /locus_tag="PLP50_01800"
     CDS             complement(54636..55751)
                     /locus_tag="PLP50_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50310.1"
                     /translation="MIGREVAARLRRNGRRELLAFPMVGAGAAYRDAGIPTDGPASDL
                     PAEGFSTLGLGRFARDLSAGWLGVQTRQMRFAVRQRGRMDLAIGVGDVVPLVACRLSR
                     APFLFVACAKSQRGARGHRWNGLETGLMRRSCGVVARDPETAARFQGCGVPVLGWGNP
                     LVELIDRAPSPEPRRKDGRPVVLLLPGSRRDAGRRVEELLDVALLLERRSGDSFDFVL
                     ALAPFNDGDALAGLVRAGASIQDEGGSPVRRALLPGGGEVAVRRDGVGAWAARCDVAI
                     GLAGTANEQAAGLGVPLVTWPTDGVFDARYVRMKRPLFGEAQVVVPPRPEAAADAILT
                     ILSDPRRREAMSRAGRARMAGGTSLRSLVARAEELLA"
     gene            55803..56027
                     /locus_tag="PLP50_01805"
     CDS             55803..56027
                     /locus_tag="PLP50_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50311.1"
                     /translation="MPPGLFRVLVPTFILFVLVRTGFDVAVRGFVDLRSSTLLEYLVV
                     PTVQGIALWLFLGGLRRRMPLHGKGRPQNR"
     gene            complement(56015..58348)
                     /locus_tag="PLP50_01810"
     CDS             complement(56015..58348)
                     /locus_tag="PLP50_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50312.1"
                     /translation="MTSTRTLASFGLVLSLAVPARADLTDVLISGQARILADTNGNGR
                     PDDGDAGFTLRRDGNRFVLQGFGGLQPQGEYVHTFELLENGERFVLQETTAYTGYQTW
                     NNPDELYELTLSRSGGAATFNRLDVTVPNPGNASGTFEQTDGGGFWDAVRGSENNGTP
                     ILADIEYYPGRSAPTHVVFAVKLFVSSPLSPDPGSLGTKQVRFFVPLRDGGVDLSDGS
                     GTILSIPMNPDAATPPKTIVPIVLDVTSGTAHFTTELSLANPGDSSTTVTLRYTASLG
                     EKLGSGTVSETIPAGGQVRFPDMISTLRTKGLAIPTTGAQGGVLVVESQGGRVVAAAR
                     TTTAVASPEGRAGLAYVGLGSADRLTGSATVYGLRSSAADRSNLAVLSTDDEPVTVRV
                     TAFNGETGASTVVREALTLPSWGWYQFDKVLDGVGFSNGWVTVERTSSGGSFSTYGVV
                     NDNSTNDGSHVPPVAATGAASGTITLPVLVETAEFRSELVVANRGASTAHLSLSYVES
                     LAPVGGAGGTVALDVPAGRQLILADAIQYLRSHGATIGAKGSASYAGAVRVTVSGVSV
                     PEVFIGARTASQAASGGQFGLFTPCVYPGQEATTEARIYGLAADSLNRTNVAVLHAGP
                     DGSGPITLRLQVLDGSSNGSPVGSPFDVTLNPGQWAQPSGFFAAAAAANGIVRVARLS
                     GTASWVAYGVVNDGGQPGKRTGDGAYVPAIVGPGLGATSTEGGLAAWMARTRGLIRLR
                     DEGHPFGARGAGLLTLIASGLVAVVLVGRRSSSGQRF"
     gene            complement(58444..58665)
                     /locus_tag="PLP50_01815"
     CDS             complement(58444..58665)
                     /locus_tag="PLP50_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(58633..58635),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HPA50313.1"
                     /translation="MKVEITYCVVUNYQPRAAGLAAELKGAFGVEATLTKGSGGVFDV
                     VVDGTLVFSKRETGRFPEPEEISRLLANR"
     gene            58805..59353
                     /locus_tag="PLP50_01820"
     CDS             58805..59353
                     /locus_tag="PLP50_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50314.1"
                     /translation="MSAARPVPRPVPPTPLEALFREYGDLVFRTAFRVAGSAADAEDV
                     LQTVFLRLASPGKAERLGAEPRGYLQRAAVNGALDLVRTRRSAAGTVPIEERADAFLA
                     KEPLPDAASEARELRGRLRRALGKLAPRPAEVFVLRALEGLPNREVAHLLGSSPATVA
                     VQFFRARTQLRKELLAEGGGPS"
     gene            59350..61908
                     /locus_tag="PLP50_01825"
     CDS             59350..61908
                     /locus_tag="PLP50_01825"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPA50315.1"
                     /translation="MRDDIFEKAVEAVRTQDPDPAAVDAALARVAPTVQAAAPAAPAA
                     AEEAPAVFRSCADLQVLLPAFLAGSLSPVRALLVEDHTRSCVPCRRALKNLREGTPSA
                     APAAPAARRRPYGAWALAASVVLAAALGTFWLVSHRAAAGPVARVDVVDGLLFLPAEG
                     RPLAPGAELAGGQQVRTGRSGGAVLVLADGSRVELADRSQVSIARGAGGTTVSLARGR
                     VIVQAAKQRSGHLFVRTEDSLVSVTGTVFAVAKGAKGTRVSVLEGEVHVDDGAGEKVL
                     HGGDQAATQGIVARVSLEEEFGWSRDRVALLELAGEVARVQNQLAAVPVSWTGRTSTR
                     LLDAMPDGTALYAALPNLSGTVADFYAALAPRLEANPVLASWWSEGPRAAERKAEVKE
                     LLDTLRTWGSYLGDEVAVGIAKDASGHPGAPIALTTVVRPGFREFVESEIARAGAGAK
                     VRIVGSPAEAAEDTLNLWLRDDLLVAAPNAGALARLESAIAAPGAFRSGAFHARLAEA
                     YRDGIDLLVGVDAKALLARAASGANPESGAFLRMSGLADVEHLIVERRSASGVSTGRA
                     ALTFGAERRGMAAWIAPPAPMGALSFVSSSATGAIAVVTKEPALLVDDLFAMLTAGQP
                     DFPVKLAEAETKLGFRLRDDLAAAIGGDAAFAVDGPLLPTPALKLVVEVYDPARLQAT
                     IVKLVSLADAEARKTGKPGVSLATESVSGLTIHSLVTGTAPSRLQYAFVDGYLVVAPE
                     KALLVRAAEAHATGDTLLTAPKLVSLLPKDGPLDFSMLAFQDVGGALGALANAVGAAP
                     GSAASGLSGSGASLAWAWAEPTRVVFGSSGAGLTELGSLVAAGAALDAPASGGR"
     gene            61905..62825
                     /locus_tag="PLP50_01830"
     CDS             61905..62825
                     /locus_tag="PLP50_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50316.1"
                     /translation="MTAFLELDGLSVRLGRRDVLRDLRGTLAGRAIGLLGPNGAGKTT
                     LLHTLLGFFPPSSGTARLAGLDVVKESREVRRAVGYMPENDAFVAGISGVRYVAMFAE
                     LSGLPRAEALERAHEALFSVGLGEVRYRNLETYSLGMKQLAKLAQAIAHGPRVLFLDE
                     PTNGLDPPARQRMIRLVKEIREAGEIRVVLSSHLLRDVEECCDEVLILKDGKIAALCD
                     LEAERRANRRLVELEAAGGNGDFAEALASLGCTVAPGIRRRLKVVLPEGVGPRELFRL
                     AGEKGVQLRHLAYQRDSLEEIFLRAMENGG"
     gene            62822..63682
                     /locus_tag="PLP50_01835"
     CDS             62822..63682
                     /locus_tag="PLP50_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPA50317.1"
                     /translation="MTVRARRFRPFEGTPTSTLTRLLVLPRYAWKEAFQAKLVTGAFT
                     ASFLAPVGALVLVWLKHNIAALANTPLAGGIPLPIDEKFFGWLLVTQSYFAFALTLFV
                     GPSLVSPDLVNGALPLYFSRPLSRWQYVLGKLLVLAALLSAITWVPLLVVFVLQASLA
                     EGAWIAEHAGIAGSILLGSLLLVLFLSLASMAVSAWVRNRAVARGLLIGLGIVPAVFG
                     QALNGILDVEWGGVLNLGLAWGRVLEGLFGIPVRAGIPLGGAWASLLTATALSLLLLR
                     RKLRAFEVVR"
     gene            63679..64716
                     /locus_tag="PLP50_01840"
     CDS             63679..64716
                     /locus_tag="PLP50_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50318.1"
                     /translation="MTGAELAWGAPSAAPRTPQPGDEAAPVSSSSYSAADLVLFEEVS
                     KFYGEVLGVDRVTLAIPPGITGLVGPNGSGKTTLMNLATGLIRPTRGRVSVLGVPPDD
                     PERLFSVCGYCTCFEAFPAGATGWSFLEGVLSLRGEGRSAARALAGKALERVGLVDAA
                     GRKVAGYSKGMKQRLRLAAAIAPDPRVLLLDEPLNGLDPMARAEVLELFRSLAAEGKH
                     VVVSSHILHEVDLLADRVVLVKSGYVVAEGESHGLREEVVERPMQVLVRCDRPGALAS
                     RLFAEGLATEVELHEDDRGLLARTRTPERFFLALNDVVLTDGLEIETVTPADDDVKAV
                     YRYLIEGGEGA"
     gene            64713..65591
                     /locus_tag="PLP50_01845"
     CDS             64713..65591
                     /locus_tag="PLP50_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50319.1"
                     /translation="MSGSRPEIATRASHEAAGAAGEARLRRDARLRQLGAVVRLELRR
                     LFRGREGKATLFFALAPVLLAVVGLFARHDPLPAARAERSFGFIFQNLVLQGTLYFGC
                     LFTFGGLVRGEQIGKTLHHLLLAPVRREVLLLGKYLAALAATATLFGVGSAVTYVALL
                     SDGGRPALAAHLGSGGAERLVAYVLVAALACAAYGALFLAFGQWVKNPAFPALAFWGW
                     EHLNFLLPEGLKRLGLIHYLLSLTPVRVPESFLAILGEPLPVWVSIPVPLLFTAALLA
                     FAAWKVRRTEVDYGIE"
     gene            complement(65576..67771)
                     /locus_tag="PLP50_01850"
     CDS             complement(65576..67771)
                     /locus_tag="PLP50_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA50320.1"
                     /translation="MTEPFERLHPAVQHHVVNSLGWASLRPLQSLSIEPVLAGDHALL
                     LAPTAQGKTEAAFLPLLSRMLTEEWRGLSVLYVCPLRALLNNLHPRLERYAGFIGRRA
                     GLWHGDVADSVRRRILADPPDVLLTTPESIEVMLTSRRVDRPWLFGNLRAVVIDELHA
                     FAGDDRGWHLLSVLERVARFAGRDVQRVGLSATIGNPEALLEWLAGSSGGSRRVVAAP
                     QGPAADADVRVDHVGSIENAAHVISRLHPDEKRLVFCDSRSRVEDLTYALREAGVEAW
                     ASHSSLSAVERRQAEARFAEGGSSVIVATSTLELGIDIGDLDRVIQVDAPGTVSSFLQ
                     RMGRTGRRNGTRRNYLFLTTSRDSHLLACGLVKLWARGFVEPVVPPPSPVHILAQQIL
                     ALTLQNGGLARGGWREWVEGMPGFRALADPNYTILDLTPRSPVCSAGERVEGHLVETG
                     FLFDDEGVLSFGPESEASFGGRRFLELLSVFSSDPLFSVRYGSEDVGRVDPATFQLAR
                     ERAAVLLLGGRPWRVTGLDWKERIAYVEPAQEAGRSLWLGSGAPLSFAVCQAVRDVLV
                     DPSSVPGLTRRGTEALRSAVDFFPALASEVTTLVQEAPEKVRWWTFAGLRANATLTER
                     LAEQGLSVTSRDNFGITLREGSTAAVSLALEKLRPTSVDPAPASLVAEALDGLKFAEC
                     VPKDLAMAMLRARLADPDGFRDTVGRPSSAVAASSAESPVDPVPPYSIP"
     gene            complement(67768..69084)
                     /gene="brxD"
                     /locus_tag="PLP50_01855"
     CDS             complement(67768..69084)
                     /gene="brxD"
                     /locus_tag="PLP50_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HPA50321.1"
                     /translation="MISPQRRADLLDALRRGTVPRNGLELLAVGLGRFESVLEEELSR
                     VRAGGSGFKAVRGEYGSGKTFFGRWLQERARKLGFATSEVQVSETETPLHQLGTVYRR
                     LVERLATDDVPEAAFRSVIDGWFFALEQDVLAEGLLKPGDHEGLLEATAGLMEKRLSA
                     IGRSAPAFAAALRAYRRALADGETEMAEGLVAWLAGQPNVAAKVKRYAFVKGEVDHFT
                     AMGFLQGLLVVLRDSGFSGLVFVLDEVETLQRMRGDVRDKALNALRQLVDEVDGGRYP
                     GLYLAVTGTPAFYDGPMGAHRLAPLAQRLHVDFSGEARFDNPRAVQIRLQGFSRPLLE
                     EVGRKVRDLYADGAADEARVRALADDGYLASLAAAVTGELGGRVGVVPRIFLKKLVAE
                     VLDRVDQFHDFDPRRDYRLTLAEAELTRQERSARAARDPDEIELPA"
     gene            complement(69103..71763)
                     /gene="pglZ"
                     /locus_tag="PLP50_01860"
     CDS             complement(69103..71763)
                     /gene="pglZ"
                     /locus_tag="PLP50_01860"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HPA50322.1"
                     /translation="MTSLPTPTHVARLVSSVRKKLPAARSIGVRYEGRWKGPETLEVG
                     DGPARVAWCPSALSAREALSTSCRDETLVLLTDRTPADLGADVLARLAKGKLFSVVPW
                     EILQDLFGAGGLDRRLLHERWMADALLEAAPPEGFPPSPTGFLDADTAWGAYLTRGLG
                     FASGAPDARGLVEWASDAGCVARFRSASSNARAAVGRRLAETIGPLATPVLLAAESAA
                     YPADALTIGLVCRVLFHAEVRTSSGLRSAAVRLEPMLGGTSLSPEIGARWADEAEALL
                     SARWKDLPPGVRFATWKAALDRADALLAALKGQAGAFLSDFLPSSLEQRLRRAGAALS
                     AGLSSGRFDLAEMERLRTDVARHLLAGQNPERADAVDAAYRLVRWIDGQRGRQRASTF
                     FGLCREFVDSSSYVDAARERLSIAESVGDLASAYRALLSRLAELREDENRAFGAELAR
                     WCSAPGSSASEIPVEEVLRRVVAPIAQGRPVLLVVLDGMSYGVFRALSPDLVGTGWKE
                     LAPEAVGRRLSALSAVPSITEVSRASLLCGALATGGSAEEKAGFSKQSDLLAACRAKR
                     PPLLFHKADLAGPGGAGVSPRVLEAVEASENRVVAAVLNAVDDHLLKGDQLNVRWSLA
                     TLQPLRALLDAASAASRVVILTADHGHVVERELAARPSPDSGERYRPPTTPPLDDEVV
                     IEGPRVPTAYGGKVVVPWSERVRYGQKKNGYHGGATPQEMVVPLAVWVATEERPAGWE
                     HLGPDLPTWWEPEAAASAPAPAVPKRAAAPKGAATEQLELPVAEDDSIARLLASAAYA
                     DQKSLHGRLQGADENVRAFLEALESFGGQATKTALARKMDLPAFRFAALLPAVRRLLN
                     VDGYPVLAVDDASGTVTLNRGLLEAQFDVR"
     gene            complement(71760..>73093)
                     /locus_tag="PLP50_01865"
     CDS             complement(71760..>73093)
                     /locus_tag="PLP50_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50323.1"
                     /translation="THELDRAIESLDPSFSPRLPVAANLGEGLGKVIDQLLSHVYPGH
                     PLFEEELKPALVRKVAEEVRRAADAVDGRIDVEKPLRDAMRKVAMPLELGTMHESAFV
                     LGRTWANHFERRIGEQPGELTVAMLRKWIDEPKPKGLPREIQDLVILVFADQSQRTFF
                     LSGAPFKVDVGTIRDELVLRSQQLPPEDTWRRASERANVVFGWAGGSHRTATNVASLI
                     EALRKREGELREAARTLRDVLLGRLQTRGIPEAESSRFATARAAVDLLEAVRKAPEMQ
                     MCEAFAGMPVPASEQALARSLSSAADVARALGDTRWETLDQALQLSGRFLVAAEALSS
                     EVDEALRKDELAVGLRNVLATAEKRALKLTGDYLASTRLAPEPKEPPLSPPPPEVERT
                     GSRVVVEEDSREVGDAASARRLFGELETKLSKQPGTRLTLRWTIEREGGKR"
BASE COUNT        10221 a        26015 c        26929 g         9928 t
ORIGIN      
        1 cccggcgcga agagagggaa ggactgatgc ccgacacgaa gaagctcctc gacgtggtga
       61 cctccgcgaa gccgggcgtc cgcttcggcc gggtctccgc actcctggac gacgcggtcc
      121 tcgtctcctg tggcgagggg tcggagctcg tcgcgtgcga cctcctcgat ctcgaggcgg
      181 gtcggtcgga cgcgctcgcc gagggcgagc gcgtcctcgt cgtggtgccc gagggcgggg
      241 aaggccgcgg cgtcgtcctc ggccgggtcg actcgccggc cgcgatcgcg gcgcgcccga
      301 aggaagcgcc ggacgagctc ctcctcgagg cgaagaaggg cctgacgctc cgcgtgggag
      361 agggctccat cacgctgcgc gaggacggga aagtcctcat ccgcggccgg gacctcgtct
      421 cgcacgcccg aaggctcaac cggatccgcg gcggctccgt ctcgatcaac tgaggacgag
      481 ccggtgatct cgtcgaaggt cgtcctgctg cgcgacgtac tcgaggagca cctcgaggag
      541 ctgccgttcc tctggcagct gcgtcgggac gccgtccgct cgccggagcg gacccgcgcc
      601 gacgtcgacc ggctcgacgc gcggatccgg gcccatgcga acggtctcct cgtcgcgcgg
      661 ggagagctcg gcgagctcct cgggccgtcc ctcgcctccg acgaggcgtg cgcggcgttc
      721 gccgccggct tcgtcctcct ccgcaccggg gaggagggcg cgcgcgccgt cctcgatgcg
      781 tttcgcgcgg cgggacccgg cggacgggaa gggctcgccg aggcgctcgc cttcggcgag
      841 gcgcgatacg cccgggagga gctggcagcg ctcgcgggct cggacgaccc gtcgctcgcc
      901 gtcgcggcgg cgaccgcgct cgcgctcggg agaggggcgg ccccgcccga cgtgacgcgg
      961 ctcctcggcg aatccgaccc cgccgtccgc gcggcggcgt ggcggctcgt gccgtaccag
     1021 gctcgcgcac cgcgcccgga gctcctgcgc aagggcctcg acgatccgga cccgggcgtc
     1081 gcgcgcgaag cggccgtggc cgcggcctgg ttccgctcgc cgctcgcgct cgaggcgagc
     1141 cgagagcggg cggccggtgc gagagacgac gccggcgagt ggctccgtct cctcgccgtc
     1201 ctcggcgagg acggcgacgt cgcggcggtc cttgccggcg cggcgaaggc ctctgcggcc
     1261 ggtcgcggcg ccgcggggcc gcgcgccctc ggggcgctcg ggcatccccg cgccgtcgag
     1321 ctcctcctcg aggcgatggg agacgaagac ccggcgcgcg ccgcggcggc cggagccgcg
     1381 ttccggaagg tgacgggatt cgacgccgcg ggcgagacgc gggtcgcgct ccgcccggac
     1441 ggtcccccgc cggacgactt cgacgaggag ttcctcgacg aagtgacgct ccccgacgcg
     1501 cagcaggcgc gcgcgttctg ggcctcggcc cgcgggaagc tcgccggagg gaagaggtgg
     1561 tgctacgggg tcgacgtcgc cgcgggcgtc cccgcggccc tcccggcgtc gttcgacatg
     1621 gagtcgtgct tcgaaatccg cctgaggggt cgattcaccg ggacgttcac gggagacgcc
     1681 ggcgagcttc tccggatggc gccgtagcgg cgcgggagcc gttcagctcc cgatgccgcg
     1741 gcggcggagc gcgtctttca ggctgacgag gactccctgg gggagctgct cgtcctcccc
     1801 gccgtccatc tcgaccgact ccttcttgta cttccagtgc gccgccccgc ctccctcggc
     1861 agcctcgtgc atcgccacgg tccggatctg cacctcgtag acgaggccgc ccggggcccg
     1921 aatgcaggta tggaggctct ggtagccgtt ctccttcggc tcggcgatgt agtccttgaa
     1981 gcggaggagg aggggctcga agcggtggtg gagcgccgag agggcgagat agcactcctc
     2041 ctcggtctcg agcaggatcc ggaacgcgaa gatgtcgtgg atctgctcga tcgcgagccc
     2101 cttcgacttc atctttcgcc agatcccggc gacgtgcttg acgcgatgct ggagcgcgcg
     2161 gggcttcacg ccggcctccg tgagcgccgc cttcagctcc gcctcccggt cccggatcca
     2221 cttgcgccgg gaggacgtga ggccggccgc gctccgctgg acgtccgcga aggcatcggg
     2281 ctcgaggacc cggaaggcgg cgtcctcgag gcgcgacttg agggcgctct cgccgagctt
     2341 ctgcgcctcg tggacgtccc tctcccagac ctcgtgcgct accgcccggg ccgccgggtc
     2401 ggaagactgg cgcgtctcga ggtagcggca gcgggccacg aggtagcggc ggaggatcct
     2461 ctttcggaag gccacctcct gaagcgaggc gccggccggc gccccgtctc ccgcggcgcg
     2521 ggccggcggc ccggcgggcg ccgcggcccg caggccggcg atcgtccgcc gctccgcctc
     2581 gacgaggcgt ctcacggtct cctcgagctg ggtgccgagg ttgcggaagc ggtaggcgag
     2641 ccagcgcatc tcccaggcgc cgcgcgggat ctcgagcccg ctccagtagc cctgctccat
     2701 ctggcggacc ccttcgagga ggccccggag cgggcgctcg atcaggaaga agtacgcgag
     2761 gtagagcgaa acgaggatgc agcccgcgcc gaccgcgagc gtcaggagcc agtgctgcca
     2821 gagccggcgg gacatcgagc ggaaccgcgc ctcgtcctgc gagacctgga gcgagccgct
     2881 gccgccgggg atgaggcgcg agcgcacggg gacacgcgcc gtcgtggcgt cgctcgacgg
     2941 gagcggcgcg gggccggtta agggcgtcgc gaagacgcgg ccctcggcgt cgaagaggag
     3001 gacgcggacg tctccgaagc cgcccgcggc gtgcgccgcc tggaatcggg tcagccggtc
     3061 ctggatcgcg gcgaggtccg aagcgtcccc gacgtcggcc gcgagagggg cgagcgtcat
     3121 cgtcaggaag cgcatccgct cctcgcgtcc ccggtcgatc tccctcttct ggacgaacgc
     3181 cgtcacgatt ccgaggacga cggtgacgat gaccgccgtc gtcaggccca atcgccagcc
     3241 gagcggaacg cccctggccg gtacgccgga aaggctcaaa gcgacctcgt cacggcaagg
     3301 accgtgcccc tgattctctc gtcacgcgcg caccggagca agcgaggccg gccggacgcc
     3361 cgaaggatgc gcgccgaacg ggccggcctc cggggcgcgg cggtgccgga acggctcagg
     3421 acgcccgaag cccggcctgt tcgaggatgg cgaggatctc ctcgcgcggg gccgcgccga
     3481 gggggaggag ggggcgccgg ggcgcgcccg cagggcggcc gaggatctcc agcgccgcct
     3541 tgaggccggg cacgccgaaa cggctcgtga ccgcgcgggc catcgggagg agtcgcaggt
     3601 ggagcgccct cgcttcgtcc agccgccccg agaggaacgc ctgccgcatc gcgacgcact
     3661 cggcgggggc gacgttcgcg gcggcgagga tcgagccggt cgtcccgacc gcgtagccgg
     3721 ggagggccga tccgtagttc cccgagaaga cgtcgaaccg cttccctccg ggcgcgagcc
     3781 ggacgagctc ggcgagcttc ccgacgtcgc ccgagctgtc cttcacgccc gccacgttcg
     3841 ggtggcgcgc gatccgcgcc accgtggcgg cgtcgacgtc gaggccggtg ttcgcgggga
     3901 cgttgtagag gaggaccggg accggggaga cgtcggcgac cgcctcgaag tgcgccgcga
     3961 gcgcttcggg cgacatcgct ccccggaaga agtgcggcgt cacgacgaga acggcgtcgg
     4021 cgccggcgcc ggccgcgcgc ttcgtcgcct cgatcgtcgc gcgcgtcgaa agccggccgg
     4081 agcccgccag gaggaatcga cccggggcgg cggcgcgcac cgtggagacg accctctcgg
     4141 cctcgtcgtc gtcgaggtgg acggcctcgc cgttcgagcc gagggcgagg aagcccgcga
     4201 gccccgtgcc ggccagctgc gtgacgagct cgacgaggag gccgagatcg aggccgccgt
     4261 cctcgcggaa cggagtgggg agaggcggga tgatcccttc catcggccga tcttatcggt
     4321 ccgcggcgcc ttcacgggcc tccggaaggg gcggccgtcg cgtcgcttgg acgcctcgcc
     4381 ggggcggagg agaatccggc gccatgaacg tcgtcgagac gccccaggcg ccgcgtcccg
     4441 ccgggcacta cgcgcaggcc gtcgtccacg gcggcctcgt cttcgtctcc ggccagctgc
     4501 ctcacgaccc gtcgcgtccg ggcgtccctc cgggcgacgt cgaggagcag acggagcgcg
     4561 cgctccgcaa cgtcgaggcg atcctcgtcg ccgccgggag cggcctcgac cggctccttt
     4621 ccgtgacggt ctacgtcgcc gacgtgtcgc tctggccgcg ggtgaacgcc gcctacgcgc
     4681 gcgtcctcgg ggcgcaccgc ccggcgcgcg ccgtcgtccc ggtgaaggag ctccaccacg
     4741 gctacctcgt cgaggtccag gccgtggccg cggtccggga gcagacgcgc gggtgacgac
     4801 gccttcgccc gtcctcccgt gggtcgccta cggcgtctgt gccgtcgtct gggggagcac
     4861 ctacttcggg atcgccctcg ctctcgagtc gttccccccg aacggcatgg tcgcggtgcg
     4921 cttcagcgtc gccgccctcc tctgcctcgg gctcggccgg ctccggaagg agccgctccc
     4981 ttcgcgccgc gagctgccgc acctcgccct cgtcggcctc ctcctcctcg gcatctgcaa
     5041 cgccctcgtc gtctggtcgg agacgcgcct cggctcgggc gtggcggccg tgctcgcggc
     5101 gctgacgccc gcctggttcg gcgtcttcac ggcccgggaa gagcctctcg gcgcgcgcgg
     5161 gtggaccggg acggccctcg gcctcgccgg cgtgacgctc ctcgccgcgc cctggtccca
     5221 gaaaggcatc gacctcggcg gcgtcggggc gctcctcctg gcgagcgcgc tctgggctct
     5281 gggaaccctt caccaccgca ggcacgtgac cgggggaggc gcgttgacga actcgggcct
     5341 cgagatgctc gcggcggccg tctacggcgt cctcctcgcg ccgttcaccg gggggttcac
     5401 gagcgggccg gtcacgacga aggcccttct cgctgtcggc tacctcgctc ttttcggctc
     5461 gtgcgtcgcc tacacggcgc acctctacgt ctcgagggtc tggagccccg tgagggccgg
     5521 cagctacgcc tacctgaacc cggtcatcgc cgtcgtcctc ggaacggcga tcctcgggga
     5581 ggcgttcgac gcgcggatgg ccctcgggat ggcggtgatc ctcgcgggcg tggcgctcgt
     5641 ccagtaccgc tcccgcgccg cctgacggcg cgggtcgtgg cctcgcggcc ggtttgattc
     5701 cgggttgggg ccggtccatc ctccccgcgc gggaggactc tcccggagga tgtcgcgatg
     5761 ctcgtttgcg gaaccagctc gaacggtcgt gcggaagacg cgcggcgcga tgtgaatgct
     5821 cccgcctcgc cgggagctcg cccttcggcg gccgccgcac gcggacgcat tccgcgcgcc
     5881 gtccggtggc gcggcctgct gctcgcgggg agcctcctgc tcgctccgtc cctcctggcc
     5941 gtcgggccca cgaccgaccc gcggcagtgg atggtcgagc tggaggggga gcccgccgtc
     6001 gaggtctgga tccgaggacg cgccgagggc tcgaggtcgg cgctcgccgc gtccgcggcg
     6061 tcgagccgcg tcgccgagct ccgcgtcgcg caggcgcgcg tcgaggagct cctcacggcg
     6121 ccggcgatcg gcgcccgggt ccagtaccgc gtgacgcgcg ccttcaacgg cctcgccgtc
     6181 gtcgccgacc cctcccgcgt cgaggcgatc cgggcgctgc cgggcgtgaa gtcggtgaag
     6241 ccgctcgtcc tccacgctcc ggcgaacgcg acgagcgtcc cacacctcgg gctgccggcc
     6301 ggcgtctggc aggccttcgc gaacgccggc gacgacgtga aggtgggcat catcgacagc
     6361 ggcctcgact accagcacgc cctgttcgga gggagcggcc gcgaatccga ctacaaggcc
     6421 aacgaccgga cgaaggtcga cgacggcttc ttcccgaccg cgcgcgtcgt cggcggctgg
     6481 gacttcgcgg gggacgccta cggcggcggg ggcgcggcga cgcccgaccc ggacccgatg
     6541 gactgcggcg ggcacggcac gcacgtcgcg ggaagcctgg gcggctccgg cgtccgcgcc
     6601 gacggcacgg cgttcgcggg gccgttcgac ggcagcgtcc ccttcggctc gatgcgaatc
     6661 ggcccgggag tcgcgccccg ggcgaagctc tacgcgctcc gcgtcttcgg ctgctccggc
     6721 tcgaccggcc tcacgaccca ggcggtcgac tgggcgatgg acccgaacga cgacggcgac
     6781 ttctccgacc acctcgacgt cctgaacctc tccctcggct ccgagttcgg ctcgaaggac
     6841 gattcgaccg ccatcgccgt cgagaacgcg gcgcgcgcgg ggatcgtcgt cgtctgctcg
     6901 gccggcaacg cgggcgacac ctatttcgtc accgggagcc cggccgccgc cgactcggcc
     6961 atctccgtcg ccgcgtcggt cgattccggg gtgacggcgt cgttcgtcgc ggtcctggct
     7021 ccgtcgacgc tcgccggctc gatggcggcc ggaacggcgt cgttcgggag cacgccagcc
     7081 gcgaacggga cgatcgggcc gctcgtcgcg gcggtcccgg cgcgggtctg cagcgccgtc
     7141 acgaacgggg ccgagctggc cgggaagatc gctctcgtcg accggggaga ctgctccttc
     7201 gacgggaagg tgaaacgggc gcaggacgcg ggggcgatcg gcgtcgtcat cgcgagcaac
     7261 gaggacgccg tcatcgggat ggcggtcgcc gacccggcgg tcgccgacgc cgtgacgatc
     7321 ccctccgtcc tcgtctcgtc gtcgaacggc aaccgcctga aggcggcgct cgccgaaggg
     7381 gtcaccgtcg cgctttactc cggctcggac acgctcgggt cgttctcctc ccggggaccg
     7441 cggctcgacg gctcgctcgc cggcccgaag ccggacatcg cggcgccggg ggtgtcgatc
     7501 acctccgccg cgacggggat cgtcgccggc gtcgccggac ccgagctgaa cgccgggaac
     7561 ctcgccgtgt cgatgtccgg aacgtcgatg gcggcccctc acctgacggg cgtgatggct
     7621 ctcctccgga agcagcgtcc cggctggacg ccggccgagc tgaaggccgc ggtgatgaac
     7681 acggccggct tcgacgtgag cctcctgccg ccggcgggct cggcgccgca atacggcccg
     7741 gcccgcgtcg gcgcggggcg ggtcgacccg gctcgggcgc tcgcgaccga ggtgatcgtc
     7801 ttcgacgacg agcgcccctc cgtcgtgagt ctctcggcgt tcctccaggg gagcacgccg
     7861 acgacggtga cgcgtcggct gcggatcctg aacaagggtt ccgtggcggc gacgctgacg
     7921 ccggggtggg ccgcgcccgc cacgatcccc ggtgtgcgcg tggacgtccc cgccgcgccg
     7981 atcaccgttc cgccgggagg ctcggcgacg ttcgacgtga gcgtcgtcct ggccgaccct
     8041 tcggcggtga cgaacgcccc cgacccgacc ctcgagctcc gccagcccag cttcgtttcg
     8101 ctttcgaacc gattctggat gacggaggcg agcggcctcg tgacgctctc gggagcgccg
     8161 gccggaacgc tccgcgtccc gctccaggtc gtcgtccgcg ggcagtcggc gatgcgggcg
     8221 gcctcgaggg cgctcggcgc gcccgctgcg ggcggcccct tcacgatccc gctcgcgggg
     8281 agcggcctga agacgggtcc gctcccgccg gtggacgccg tctcgacggt gtccgcgttc
     8341 gagctgcacc tcgatagccc ccggtcgagc ccggagggaa gcgcgcgcgc cgtcgcggac
     8401 ctcgccttcg tcggcgccgc gacgtcggcc ctcgcggggc gactcgactc cggcgcgacc
     8461 gtctggttcg ggatcgcgac ctgggccgag tggctgagcc cggccgaggt ccggttcgag
     8521 atcgaggtcg acaggaacgg cgacggcatc gtcgacgcga cggtggagac gatggcgacg
     8581 ggcgacgtgg cgaacccgtc ggacccgtcg cagacggtcg acccgacgga cgtatgggtc
     8641 gcccgcacgg cgccggctct cggcgcgggc gcggcgagct accgctacct gaacgtcgat
     8701 cccgcttccc gcggcacgca cctcttcgcc tccaacgtcg tcgtcctgcc cgcctcggcc
     8761 ggcgcggacg gcctcggcct gacggccggg aacgcccgct tccgctatcg ggtgaaagcc
     8821 tcgacgtggc gcagcggcac cgtcgaggag acctcctggc tgacgttcga cgccgagaag
     8881 ccgggttact cggtcctcgc ggccgacggc acgccgttct ccgacgacct cccgggctcc
     8941 gccctcccgg cgcgcttcga cccggcggca gcgcaggcga acggtgccct cggcgtcctc
     9001 ctgcttcacc accacaacgc ctatggagcg cgcgccgagg tcctgagcgg gaggaacagc
     9061 ccgccggccg tcacgatcct ggagccggcg ccgggcgccg cgttcgacgc ggccgcgccg
     9121 gtccgattcc gcgcctctgg gaccgacgcc gacacgggcg acacgctcgg gttctcctgg
     9181 agcctgggcg acggaagaac cgtctctggc gccgacgtca cgtccgcgtt cgggagcgcc
     9241 gggccgcgcg tcgtgacggt gaccgcgacc gacggcgccg gcgcgacggc ctcggcgcag
     9301 gtgacgatcg aggtccgggc ccctcaggcg ccgtcgggga tctcgaagct cgtccccgtc
     9361 gtcctcgacg tgaagggggt cggaggcgcg ccgttcacga cggagctgac gctcgtctcg
     9421 aggtcggcga ccgccgcacg ggcgctcctc gcctacaccg cctcggcggg gtccggctcc
     9481 ggctgggccg gagtcgacct ggggcccggc gagacgaagg tcctgccgga cgccatcgag
     9541 ttcctccgcg cccagggcct cgcaattccc gacgacggct ccaaccagat cgggacgctc
     9601 cgcgtgacgc tccccggcgc gacggagccg gccgccctct tcgtcggcgg ccggacctcg
     9661 actcccggcg agggcggagc cttcggcctc ttctacgcgg acgccgtcgc gtcggccggg
     9721 gaagcgatcg tcgcggggct tcagcagaac agcgcgatgc gctcgaacgt caccgtcgtg
     9781 aacgcgggac cggagccggt gaagctccgc gtgaggctcg aaggggcgag cggggaggac
     9841 ctgggcgtcc tcgacgagcc gacgctcccg gcgtggggct ggaagcagta cgaccagccg
     9901 ctcaagggcc gggccgcctc ggggcgggcc cgcgtctcgc gcctctccgg aagcgcgccg
     9961 ttctcggcct acggcgtgct gaacgacgcg gggacctccg acggatcgta cgtcccgccg
    10021 ctcctccccg gggctccggg cgcgggtgac cggctcatcc cggtcgtcct cgacgtgaag
    10081 gggcttggcg tcaaccgcta cgcgaccgag gtgacgttga cgaacctcgg cggcgccccg
    10141 ctcgccacga cgctcgtcta cgcggccgcg ctcggcggcg ggtcgggaga ggctccggtc
    10201 gcgctcgccg ggggagagca gaaggtgatt cccgacgtca tcgcgtacct gcgcggcgcg
    10261 ggcctcgcga tcccggcggg cggcgcgaac gtggccggct cgcttctcgt acgggtccct
    10321 tccggcacct cgcccgacct cctcgccgcc ggggcgcgcg tctcgactcg ctcgagcgtc
    10381 cgtgacggag ccttcggcgt cttctacccg ggcctggctc tttccgaggc ggcgaacggg
    10441 ccgacgtggg tccacggcct tcagcaggac gcgaagacgc gctcgaacgt ggccctcgtg
    10501 aacttcggcg acgcggggaa cgtcacgctc cgcctgacgt tccacggcgc gaacgggctg
    10561 gcgctcgccg atcccgaagt gcacaccctc gcccccggcg cctggctcca gcgcaacgag
    10621 ccgctcctct cgcgcggcgc cgctgccggc tcggtgaagg tcgagcggat ctcggggacg
    10681 tcgcggttcg tcgcctacgg cgtcctgaac gacgccgcga cctcggacgg gagctacctc
    10741 ccgatggccc gctgaggcgg ccctgcacgc gccctcccgc gacgggcggg cgcgtgcgag
    10801 gtcccgacga gcttcaggcc tccccggccc tccgcgacgc gaggaacgcc gggagggcct
    10861 ccttcgcggc gccgtagccg gcctcgacga gctccgccca gcgcccgtac tcgttcagcc
    10921 ggaaccggtc cacgggcggc tcgacgagga ggtcgacgaa tcggagcccc gagcggagcg
    10981 ccatgacgct gttgatctcc tgcgttcgga ggagcgagcc gacgagcgaa ggcgctcgcg
    11041 tcttccgcgc gaagggaagg acgcgtcccc ggaggacctt ccagcccgag agctccgggc
    11101 cgaaccggta gggcttcacc ttgtcgacgg ccggggcgac gttcacgccg atgaccgttc
    11161 cgcagtcgag ccgggacgcc atccgctcca ccgggaagtt gtcgacgacc ccgccgtcga
    11221 cgacgacgtc gccctcctcg atcaccgggg ggaagacgcc ggggatcgac atgctcttgc
    11281 ggacggctcg ccagagcggg ccgctcctct cgacgtcgac ccgcgcccgg gtcaggttcg
    11341 tcgagacgca gaagaacggc gtccagaggt cctcgatcgc gcggtcgccg tagagcgcct
    11401 tcatcacgcg caggaccttc cctccggaca tcagcgagac gagcgggagc gtccggtcgt
    11461 agatcctctt cggcgaggcg aacgtctcgg cgagcgcgag gagcggcccg gaggcgccgt
    11521 gcagggcgaa gctggcgccg acgacggctc ccatgctggt cccggccacg aggtcgatct
    11581 cgacgccggc ctcctcgagc gcccggatga cgccgacgtg cgccacgccg cgcgcgccgc
    11641 cgccgccgag aacgagcgcg acgccgcgcc ccgcgagccg ccgcgcgagg cggacctcgt
    11701 cgcgggcgtt cccgggccgc aggtggtggt ggtccgcgag gtcgcgtccg gcgagccagg
    11761 cggcggtccc gcggggctcc gtcgtctccc cggcgtggag gaggacgagc tccttcctcg
    11821 ccgggaggcc catcgcggcg agccgcgcgt cgaccgcccc gggggccggg tcggcggtcg
    11881 cgtcggcgac gaggaggacg cggtccgcgc gcctcaggca gcgctcccgc cacgcctcgc
    11941 cgccggaccc cgcgtcgagg acgaggtggc gtgtccggtc ctcgagctcg cgcagcgaga
    12001 cgtcgacggc cagaccgagc ggcccggcga gcggggattc cgcgacgcct ggccgcccgt
    12061 gacgcgcgtc gaggccggcg gcgtcgagga ggagcgtcgc gccgagcgcc tcgagccggg
    12121 acgcgagcgt ccgcgcgacg gcggaggccc cggccgtcgg cccgaggggc aggaccgcga
    12181 tcgtgagtcc gtccggaacg ggggcggacg cgcggccttt cggcgccacg gcgctcgcct
    12241 ggcgcccggc gacgatcgac gcgacgcgca ggagcgcttc cggccgcttc agggcgaagc
    12301 cgcggaacgc gtcgcgcccg aggcggagca cctcgccgtc gcggagggcg acgacgctgg
    12361 ccgagcgcgg ctggtccgcg aggagtccga gctcgccgac ggtgctgaag gcgcctgtct
    12421 cccgcacggt cgtctcgacg ccgtcctcgc cgcggaccgt cgtccggaag cggccggtga
    12481 cgacgacgta catcgcgtcg ctcgggtcgc cctgccggac gaggagctcg cccgcggcga
    12541 cgcgccgcca ctcgacggcc ttctccaggt cgcgaaaggc ctccggctcg gtgatgccga
    12601 agagctcctc ccagacgcgc aggagctgcg cgcgtcgcag gcgcggaccg atcgcctccg
    12661 tcagcctgga ggcgacggcg gggtagcggc gcgcgagctc gtcccagccc tcgcggggaa
    12721 gcctcacgag ggctccgtcc tccatcgccg tcagcgtcgc cgagcggggc tggcccgtca
    12781 ggatcgccat ctcgcccgtc cactccccgg gaccgatctc gccgagcgtc acttcggctc
    12841 cggcgggcgt cgtcccggtg acgcggaagc gaccgtcggc gacgacgtac atcgcgtcgc
    12901 ccggctctcc ccgccggaag accgtcgtcc cggcggggac gcggagcgtc gagacgaggg
    12961 cgtcgacggc ggcgcgatcc accgcgggga gagcgtcgta ctccggcgag cggaagagcg
    13021 aagcgccgtc cccgggccgc gcggctcgtt cgtgcatgtg aatccctcct ggaggcggtc
    13081 tttgtgtggt ttcggccgca tcctaaccga tgagacgaga tctccaggga tcgcggcgga
    13141 tcgcacggca ggagctcccg ccgtgtccgg aggcctcgtc cggtccgggg gccgatcctc
    13201 ctcgcagggg ccgccgcctc gccgtcacgc cgggcttccg catcgggagg gccgaggcgc
    13261 cgcgggcgcg ctcggcgacc gaatccgacc cgatctcccg gcaggggtcg tactcgaatt
    13321 ggctggacga cgaatccgcg tcgggcggaa gatggccgat ggccgcggca cgcccgcggg
    13381 agatcggagt gtcaggagga acgtcgatgc gaatcgggaa acggattacg accctcgcgc
    13441 tcaccgcgtt cgtcacggcg gcgctctccg gctgcggagg ggccaaggag tcccccgagc
    13501 cggccggcga gccggccgcg gccgcgccag cggaggcccc ggcggcgagc ggcgccgcga
    13561 agatccacct gccgacgggc tgggcgatgc aggacgcgat ctccgccgcg gacgtcggcg
    13621 cgatcaccgg ccagacgatg acctacttcc ccgacgcggg gagcaaggcg caggaagggc
    13681 ggccgtccgc cgggtacaac gtccccggga aggacttctc cgtcatccgg ttcgccgccg
    13741 tcgtcggcgg aggagacgcc gagttcgaga gcacgaaggg cttcgccgcg cccggctcgg
    13801 tcgtcgaggt ggctggcgtg ggtgacaagg cctacatgtg cgacttcgtc accggcgcga
    13861 cggtgatcgt cgtccagaag ggtccggacg tccttcgcgt cgactggcag ccgaagacgt
    13921 acgccgagca cgacaaggtc gagctcggaa agaagctcgc gggcaagctg atcgagaacc
    13981 tctacaagta gcgcccgata cgggccggaa acccttctgc ggggactcgg cggcgagccg
    14041 ggtccccgct tgattcctcg ccctgtgggc gagcccggcg gctactcgcg gagcgtcgcg
    14101 gcgatcgccg cgatcgccgc ctcggcctcc tccgagaaac cgggggcgag tacgaaggcg
    14161 aggacgacct tctcgccccc cttcttcgtc gcgacgtaga cctcggcgag cgacggcgcc
    14221 gggatcttct tctcgagcgc ctgcccggtc accttcctcc cgaggaggtt ccgctcgacg
    14281 ggctttccgg cggccgaggt cgcgaggaac gtcgtcttca cgtaatcgag gagctcggcg
    14341 tcggacatcc cggagtcctt cgggaaggcg acagccgtga ggctcagctt ctcgcctccc
    14401 gccttcgcgt cggccgggtg gacgacgcgg agcgcgtcga ggccggcctc ttccggcgcg
    14461 gagaagggga tcggcggagc ggccttcagt ccgtgcagcg tcaggtccgc ggcgcgagcg
    14521 cccggcgcga cgacgaccga cgccaggacc gtggcgagaa gggcgaggca gggtcgacga
    14581 atcatcttct ccctcccggg gcggcgccta ctcctctcga cactccagcg cgtcgcgctc
    14641 gaggaggccc gcggtggtct gcttcttccc gacgtacctg acgaggagcc aggccggggt
    14701 gctctcgttg acgaggccga cggcctcgac gacgtctccc ttcacgacga accccttgcg
    14761 cggctcgggg gacggggcga cggtgaggaa ccgggccttc ggcgtggtca cggtgcagct
    14821 gaaggcgggc ttccaacctt ccttcgtgaa cgtcgccccc ggccagttcg cgccgcagaa
    14881 ggaggcgatc cctttcgtcg cctccatcga caccgtcccg cccttcgtcg tgaaggtgac
    14941 ggtcccctcg tcgtcgacgt actcccacgt cccgtcgccc acgaagcccg cgacgccgat
    15001 gccgtcgcag atgtgggccg ttgcgccgaa gacgccggag taggagaagg agagcgtgtc
    15061 gcccgcgcgg acgaggacga ggggcccctg ctgcccgtac cagacgccgg tcgggtcgaa
    15121 gccccggtcg tcggaggtct gggcgagtag gggtgccgcg gcgagcgacg cgacgaggag
    15181 gagtacggct cgacggggcc tcatgggcgc ctcctgaatt gccgaatcga ttccgattgg
    15241 cgagcatagc agcacgagcg agaatcgtcc ggtccgaagg aggccggcat gaggcgagcg
    15301 cttcccgtcg tcttcgcggc gctcctcttc ccggcgaccc cggccggggg agcggagccc
    15361 gaccgcagga tcgtctccgc cgccgcggtc gccctccgcg aggcgccggg cgtctcgcgg
    15421 agggtcgtcg cgcggctccc cctcgggact ctcgtcgtgg aggtcgcccg aacgggaggc
    15481 gtcgacacga tcggcggcga gagggcgccg tggctcgagg tcgccacgtc ctcgggcgag
    15541 caggggtggc tcttcggcgc cctcagcctg ccgtacgcgg aggcggagcg cgacgccctc
    15601 gccctggaga tcgcgggcga caggctgaag cgggcgggag acccgttcga ggcgttcgcc
    15661 gagctgcacg cgttcctcgc gcgggccgca ggggaggccg cgacgccgga ggcgcgcgcc
    15721 cggatcgagc tcgccacgct gcgcgccctc gatcgggcct gcggcgcgat cggcttccgg
    15781 cggcgtcgcg aagagaaggt cctcgcgtgg gtcgaggcgc aggcgcgcga cctctcctgg
    15841 aacgagccgg cggcccgctg gatcgtcgtg gcggaccgtt tctgggagct gcacgatcgc
    15901 tccgcgtcac tttccgtggc ggacgagatc gcctgggagg ccgcccggac gcagctcccc
    15961 ggcgagtgcg agggagacgt cccgtgccgc ttctccgtgc tcggccggac cgacgtccgc
    16021 tatctcgagc ggcatccgcg gggcgcccac gccggggagg cgctcgcgaa cgtcgcgacc
    16081 gcgctcgagg aggcggtcgg ggacccgcgc ggagcggctc tcttcgccgg ctcgccgcgg
    16141 gaagacctgg agaggacgag gaacgacctc gagaagctga aggcgcttct cgcccggatc
    16201 gacgacccgc gcgccggtgc ggcgcgctcc gcagccgacg agctcctcgc gcgtgcggcc
    16261 gtggcgggag ggaagggatg agcgggccgc cgaccggggc gggcggcatg ccgtccgccg
    16321 cagcctcggc ggagcggaac ccggcgcgca tcgtcgccta cgtgacttct gccgtcggcg
    16381 ccctcacggg gccggtgtcg tttctcgcct tccccggctt cggcgtctgg acgaccgagg
    16441 acctgccggg cgcgggggtc gtcgcgctcg ccgctctcgt catggcgagc cctttcgcgg
    16501 cgttcctcct cgcggcccgc ctctcgaagt ccaggaccgg cgcggccgtc gtcgccgccc
    16561 tcgccctcgt ggccgtcctc ttcggggcgc tctactacgt ctgggcggcc gtcgagaaga
    16621 acgtcctcat cctcctcgcg tcggtcggcg tccccgcggt ccagtggctc atggcgctcc
    16681 cggccgtggt cgcggctctc gtcctgcggc caaagaggtg gtccgccccc cgctgagggc
    16741 tcgcccggtc cggcgggcgc tcaccacggg tcggtcggcc ccgggcactc cgggtatggc
    16801 tgctggaacg ccatgctccg gcccggcttc cgccggctgt cggacacctt cccgaacgac
    16861 ctcgagcccc agaagcggtc gcactctccg ccgaggaaga cggtgaccgc gctccccttc
    16921 cggtcgacca tccccgcgtg ctgggccgcg tcgcagacgt tcgagccgag gaagtagacg
    16981 cgcgtccccc agagcgaccc cttcccgtac ttgtcgtacg cgcaggtgca gtcgaggctc
    17041 gagccctcgg gcatcgcctc cagctccttg ctgaacaggc ccggacaggg cttcaggtcc
    17101 gcggcggtgc tcggcggggg cgggcagggg ggcggctcgg ccgtgaagga gaaggtcgcc
    17161 gtcggggacg cgaagttctt cgagacgacg ccgttcgccg ccgagcccca gaggcggggg
    17221 cagtcgggct gccgccagaa cgtgacgacg tcgcccgggc gggtgagaac cccggcatgg
    17281 cgggcggcct cacagaggga cgacgacggg tcgtagcggc cgttcccgcg gatgccgatg
    17341 ccgtagtccc tcgtgcagac acaggaccgc gactccccga cggccatctc gccgatgttc
    17401 gagaacagcg aagggcattg cgggaggagt ccggaggacg ccgctgccgg cggtgcggcc
    17461 gcggtctcgc cggcggagga cggcgtcgac acggacgccc gcggcgcctc gcccgccgac
    17521 gccgacggcg ccgggacggc cgggtcgtcg cggtacgcgg agatcctcgt gatcggccca
    17581 cccgccttcc cctcgaagcg cacccggacc cggaccggcc atcgcgcccc gtcggccttc
    17641 ctcggcgtcg cgagcccgag gacggccttc ggctcgccgg gggccgggga gagctcgagc
    17701 tttccgagct gctgctcgac cacggatacc ttcgcggaga gcggcgggtc gaagagccac
    17761 tcgacctcgc gcgccgccgc catacccggg ctcttcgtga cgacgatccg cgccccgttc
    17821 gggaccttcg ctatggcgcg gaggctggtg gcgtcctcgg actcgaccgt gccgagggac
    17881 ccggcgttcc cgtacaggtc gagcatgctc gcggccggga gctcggccac ctggaggctc
    17941 tgggccgtcg cgtagacgtg gtgaaggacg tcgcggacct cggcgacgct cccgggtccc
    18001 ctcgtcggcc ggcacgactt ctgcgcgacg acgatgatcc ccgcgatcgc ggcgatcgcg
    18061 agcgaggtgg cgagcatggc gccgagcgcc agggcgcctg gattgacggc gaagcccatt
    18121 cgccgcacgg aatcgacctg ctgctcgagg cgcgtcggcg cgaccgggac ctccggcggc
    18181 aggacgcgct cggctatgtc ggcgagcgcg acgagcgcgt cggcccagtc cttcacctgc
    18241 gccgtcgagg ggatccagct catgaaagcg gtcgagaggt ggaacgtgac gttgagcgcc
    18301 ccggggcgga ggacgagctg tctgcgggcg aagggcccct ggtaccgcag gagcctcgcg
    18361 aggagcgtcg ggacacccgg ctcggcgagg aggcgccgca cccacgcctc ctcctcgccg
    18421 aagacgtcga ggccgtcgaa cgcggggacg gcgaagcgga tcggcttctc gcccgccagc
    18481 gccgcgagga gctgcgtgtc gcccgagccg gccgtgatgc cgaacctcgt ccagacggcc
    18541 acgtcggctt cgagggcgag gcgcgggccg cgcgagaaga agccctggaa gcgacggcct
    18601 ccgtacgtcc cgtcgtatcg gcggtactgg acggcgtagg ggaagccggc gaggccgagg
    18661 gcgccgaaca tcctgtcgaa cgcggcgttg cgccaacgcg tcaccaggta cgcgacgagc
    18721 gccggcagga ccagaaggaa gaaggcgccg atcccgagcg gcaggaagat ctccttcggg
    18781 tcggcacccg gcgtgcgatg catccgcccg agcggaagca ggaggaaccc gacgaggatc
    18841 gtgaagacgt agatcgtgac gaccccgctg acgaggcagc ctcggacgta cgacattgcg
    18901 acggactgga cgattcgacc catcacggcc tccccggacg gaaacgtgca aaaggggcgg
    18961 acggtctgcc aaagagcaat tctacgccgc gccggggagg cggccgacgt tcggtccgcg
    19021 ctacgatgcc cggccgtgac cgacttcacg ttcctctgca acgagtgcgg cgcccgctac
    19081 ggcgaggacc gctcgctcta cgtctgtccc gcctgcgcgc gcctccaggc cccgggctcc
    19141 ttcacgcgcg gcgtcctccg cgtcgagctc gccgagcagt ccctccctcg ccgatggccg
    19201 gacgcgcctc tcgcctctcc agaggggctc gccccgttcc ttccggtccg cggcccggac
    19261 tcgttcttct ccttcgccgc gggcgggacg ccgctcctcc cggtgccgcg cctgcgtgag
    19321 gcgctccgga tgccgcgcct cttcctgaag gacgacacgc ggaacccctc gggctcgacg
    19381 aaggaccgcg cctcggccct cgtcgtcgcg aaggcgatcg agtacggata cgaaaccgtc
    19441 gccgccgcct cgacgggcaa cgccgcgacg gccctctcgg cggccggggc ggcctcgggc
    19501 gtgaagacgg tcgtcttcgt cccggcctcg gccccgcccg ccaagctcgt ccagatggcg
    19561 agctacggcg cgcggctcgt cccggtcgcg gggacgtacg accaggcttt cgagctctcg
    19621 ctcgaggcct gcgcccgctt cggctggtac aacaggaaca cggcgctgaa tcccttcacc
    19681 gtcgagggga agaagacggc tgcgctcgag atcgcgcgag gcctcgggcg tgaggcgccg
    19741 gacgccgtcg tcgtcccgac gggggacggc gtcatcgtcg cgggcgtcgc gaaagggttc
    19801 gccgacctcg tccgctgcgg gctcctcccg cgcgtcccgc gtctcgtcgc cgtccagccc
    19861 gagcgctccg gcgcgatcgc ccgcgccctc cgctccggcg ccgccgagat cacgcccgag
    19921 ccggacgccg ggagcgtcgc cgacagcctc accgtcgccg cgccgcggaa cgccgtgatg
    19981 gccctcgccg acgtccgcgc ctcgggcggc tgcggggtcc tcgtctccga ggcggcgatc
    20041 ctcgacgcga tcccgcgcct cgccgccgcg acgtccgtct tcgccgagcc cgccgcggcg
    20101 atcgccctcg cgggcctcga gctcgcgctc gcggaaggga aggtgggtcg cgaggagcgc
    20161 gtcgtcctcc tcgtcaccgg gaccggactg aaggacgtgc cggcggccgc gcggcgcgtg
    20221 aaggtgccgg agcctctcga gccgacgatc gcggcggtcg aggcgcttct ccgatagcga
    20281 ggcgtcggcc gtcagcgcgg cgcggcgtcg cgcacggtca cgtcgatgac gacagggggc
    20341 ggcgcggcgt cgcggctcgc gggcttcggc gcgagcgtga cgcgcgcggc tccgggtcgc
    20401 actgcctcga gctcgtagtg gagcgtcgtc acgccgccgt cgtcctcggg cgggggcgcc
    20461 tcgacgcgcc gcctcacgaa gcggaccgcg tccccgtcga tcgccggatc ggcgggccac
    20521 tccacctgga gctgctcggg gatcccgacg gggcgggtca cgacgaggtc gaggcgctcg
    20581 ccggcaacgg tttcgacggc cttctcgttc cggacgacgc gcatgcgggc aggccctcct
    20641 tcatcgggtc gcgcgggcgc cgcggggctc gccgcggcgt cgcgcgcctc gctgccggcg
    20701 caggcggcgg cggagaagag agtcgcgaga gccagggcga tggcgagagg ggcggggcac
    20761 cgcgtcacga tcccgaaggt accaccgacg cgcgcgtccg atgagcgtcc ggagccctcg
    20821 gaaggcgaaa ggctcgaaac ggctcgcatc gtctgagatt ggatctcgcg gcgtcgtcgc
    20881 ttgaccgggg gcttcgctcc tgcggatgat cgccggaagg gctatctgcc aattcgcgtg
    20941 acggtcgccc gaaggacgtc gagtcgtggc gaccggaatc ggagggtcgg catgcatctc
    21001 ttctccgctc ggctcctgtc ggccgcggcg ctcgccgtgg cgctcctcgt accgttcgcc
    21061 gcttcggccc agaaggcgcc gccgaacgac gtgacgaagg cggagttcta cctgaagaag
    21121 ctcgaggacc acgcccggcg ggcccgcggc gcggctttca ggccgggcta cgaggacaac
    21181 gaggcgctga agcggatcaa ggagctgaag gagaagtacc ccgacgaccc ggccgtcgag
    21241 gcgctcttcg cgcgggcgcg gacggcgctc atggggagca agggggactt cgtcgagatc
    21301 acccccgaga tgctccgcta ccgcgaccag gagaggaaga tcgtcgagct cttctagaag
    21361 gaggccgcgg cgcagatcgc ggcgtggaag gggcgggccg gcccccggct cctcgcccag
    21421 gccctcccgg cgcccggctt ccgcgagccg gcggggcggg gaatgcccgg gaagtacgtc
    21481 gtcctcgacg ggctcgagta ccccgcgaac cagttcacgg acctcggccg cgagttcgtc
    21541 ttttggggca cgcccgcccg ggggatgtac tacgtccaga tcgacacgcc ctcctggcgt
    21601 ggcgcgtacg aggcgctcaa gcgctaccgg cgcctcgtcg acaccggtct cgccgagggg
    21661 acgccgttct cggtggcagg ccggatcgag ggggccgagc tcgccgtccc ggaggcggga
    21721 aggaagaggt tcggcggcgc cttctggggc tgggtcgtcg tcccggaggc gatctgggtc
    21781 gacgggcgcg ccttcgccgt cgcggaccgg aacgccgagc tgggcgggcg cttcgcgggc
    21841 gaagagcgga tggaggagat cacgggtccg ctctacagcg tgaagtcgat ccccgcggac
    21901 gtcacgccgg agcggctcgt cgagatcttc gcgacggcga tcaaggagaa gaacctgaag
    21961 ctctacctcg actgcatcga cccgaagcgg ctccaggggc cgcgggcgag gagcctcgcg
    22021 atgtaccact gggactggca ccaggtgcgg ttccgcgact tctacgtcca cacgacggtc
    22081 gagaaggtcg agaagacgat ggtgaagggg tacgacgccg ggaacgacct cgagtcgttc
    22141 ttcctgacgg acgagcagaa ggcgcaggtg aagaagatca gcgagccgtc ggtcgagcgg
    22201 gcgaccgtca cgacgaaggc gtgggacgag cgtggccggc agtacggatc gccgaaaccg
    22261 cacgagctga tccgccgcgg cggcggtcgc tggtacgtct acgacttcgc cgcgcagttc
    22321 tgaggaggac gagatgaaga cgaccctctc gcagaacctc ccggtccgcc tcaccgcgct
    22381 cctgggagct gcgttcctcc tcgggacgaa cgggctcgct caggttccgg actcgcttca
    22441 cccggacgtc aggaacccgt tccaggagga gaagaaggac acgcgcgaga agacgtacgt
    22501 ccagaagacg gcgctccgct gggagctctc ccgccgtcac tccgagcacg ttatcgagga
    22561 gtacctcccg aagttcgagg cgatcgtcaa gcggaagccc gggtcgagcg acgtcacgga
    22621 gtggaggagt ctcggcggac agctcttcgt gtcgtgggag gagtcgagga agcacgagaa
    22681 gacggggacg cggccgtcgg accaggacga gtgggagaag cagcacaact cgttcctggg
    22741 gggaatctcc cgcaggccga cgctcctgga ttggcgggag ggcgtcgcca tctcggccgc
    22801 ggccttccag aaggaggccg acgaggctcc gggacggttc gaggagatac gcaggaacat
    22861 gcaggcgacg gacccggtct gggcgaaggc gatcgagatc gcggcgggcg ggtccgggac
    22921 ggcgtggaac agggagaagc tgaaggaggc tcaggacgcc tacgcgaacc tgaagcggat
    22981 gcgggaccag gtcgactcga cctactcggc catcacgtgg gcgtacgcgt acgccgcgaa
    23041 ggacaacgcc gagaagccca ggactcgcct gaccacggac gagcaggcgg ccctgaagag
    23101 ctggaagacg gccgtttcca cgtatccgct cgtccagaag atctacacgc agccggtcca
    23161 ggaatgggcg aagctcttcg aggcggcgtt cgacgccgag gtgaaggcct gggaggcgat
    23221 gcagaaggcc ttcgagcccg tgaagtcggg ctcgatccgg gcctcctcgc cctacaagga
    23281 ctgctcctgg gacaagaggg tcgcggagtg cgtccagaag accctggacg acctcgcgtc
    23341 ggcgatcgcg ggggcggctc ggaagatcga aggagcggac gcgaaggtct cggaggacaa
    23401 ggccctgacg gagaaggaat accgcaggct cgtcgagctg ggaaatctcg taccgccggg
    23461 ggagtggagg aagctggagc tggcggaagc gcgggccagg acggacgagg agctggccga
    23521 cgccgaacgg aagataacgg agttccgcgc taccgcgaag aaggtggccg acgagagcgc
    23581 gaagatcaag gccgagcaca aggcccggcg gaaggcgctc ggaatcccgc ccgaggactg
    23641 ggcggactga ggcgcgcgtc ccggtcgaag gtcgggaaca ggaggtcgtc atgagccgtc
    23701 gctcgctgtt ctggctcgtc gtcgtggccg ccgtcgcgtt ctggctctgg aagccggggg
    23761 aggtctcgaa cgaagggtcg gcgatccgta cgtcgtcggc gggcacgagc gtcggcgcac
    23821 cctcggccac gggagaggcc tgtctctacg ccgccgagga ggcgagccgc ctcacgcagg
    23881 aggccgcgga cgtcctcctg cgcgcgccga tcgacactgc ggtgttctcg gcggcttcgg
    23941 agagggcctc ggcggcgatc gcctccgccg aggggatgtg cgggggaggc tcctcggcgg
    24001 gggagcagcg ggcgatggag gaggcgcggg gcgcgctctc ggagatgcga ggcctgctct
    24061 cggacttcgc cggaacgctc tccggctccg gcagcgcctc ggacgcgccc cgccgtcgtg
    24121 agacgatcga cgcgcggctc gtggccgccc gggcggccct tcggggctga gcggcggcgc
    24181 cgggccgaaa cacacgatcg aggtgaacga gttgagcgaa gcgaagaagc ccggcgaagg
    24241 cctgaaggtc gtcatccgtc tccggatgtc cagccacgac gcccactacg gcggcaacct
    24301 cgtcgacggg gcccggatgc tcgggctgtt cggagacgtc gcgacggagc tgctgatccg
    24361 gcacgacggc gacgaagggc tcttccgggc ctacgactcc gtcgacttcc tcgcgccggt
    24421 ctacgcgggg gactacatcg aggccgaagg cacgatcacg gccgctggaa acacgagccg
    24481 gaagatggcc ttcgaggcac ggaaggtgat ccgctcggcg cccgaggtga acgactccgc
    24541 ggccgagatc ctcgagcctc cggtcgtcgt ctgccgggcg accggcacct gcgtcacgcc
    24601 gaaggagaag cagcggagag gctgacctcc gcgattcgct ccgggtcaac gggccccgga
    24661 cactccgccc ggtcccgacc cgctcctgac ccggtcagtg gcaggtccgg cagagctgcc
    24721 ccctgtcgtt cttcacggcg aggtagcggg tcgggtactc cttcgttcgg agcgcgatcc
    24781 tcgcgaacag gctccgggac tcccacggcg cggtgtcccg ccgcggcctc gtcgtgtcgt
    24841 ggcaggtgac gcaggtgttc gttccgccgg ccgtgaggat caacggtgcg gggacggatc
    24901 gcgggatccg gaccccgacc gggtggtgcc cttcgcgcgg ctcgtggcac gtcgcgcaga
    24961 gcgtccggca ggggcgtccg acccgcgggg agatcgccgc ggcttcgtcg gaccggtgac
    25021 aagagaagca gctctcgacg gtccacgcgg ggtgcccgtc cgcgggcggg gcgctctctg
    25081 cggtgccggg accggctgcg acgatcgcag cgacggcgag gatcagagcg gcggatctgg
    25141 gcgtcatggc ggtcctccta gaacgcgacg acggcctggg cgaggagggc gtcgcggcgg
    25201 aagccctcct ccgcctcggc ttcgggaggg gcggcgagga cccgctggta ctcgagcttg
    25261 agcgtgagcg tgtcgaagag gagcgtgcgc aggccggcgg tgagccgctc ggtacggccg
    25321 gcctcgaccc cgacctcgcc tcccgccgcc tcgggctcgg ccttcgccgt gtcccagcgg
    25381 acgtagggag agagagggac cccggccacg gcgccggcct cgacgatggc ggtgacgtga
    25441 agccccgacc ggacgagtcg ggcgtcgcgt ccctcgacgg gccgctcgtc gcggcgcgcc
    25501 cactcggcgc gaagctggcc gaacgtcgcg cggagctcgg cgtcgacggc gagagaggtc
    25561 gtctcggtgt tcccgttccc gtcggaatcg tgcagcgcgg agatgccgag ctcgaagacg
    25621 cgcgtcctgt cccggagccg gtagacgtcg tcgaacggca tgagcgcgat ccggccgccg
    25681 acggccgtcc cctcgccctc gccgcggagc cagtaggccg tccagccgag gctccccgtt
    25741 ccgccgagga gccgcagtcc gacgtcgttg tagccgccgt cgaggaccgc ctcggtcgtc
    25801 aggggggccg agagctcggt ccggtctttc gccgcgaagt actgccagtc gccgccgaac
    25861 gggacgtcga atcgcccgag ctgggcgtga aagcccttct cgaccggaag ccgcccgcgg
    25921 ggcgccacgg gagcgccgac gacgtggacg tcgacgaatc cgacggcgag ctcggctccg
    25981 ccgtcgttca cgacgagcgc cgcggcgacc tgaacgtgcc ggccgaggtc gcgttcgagg
    26041 tcgagctcga agtctcccat cgagaagacg ttcgggtcgg tgcgcgtgtc ggcggccttc
    26101 acgtcgaaga agccggagag gccgagggcg tcccggatct ccgcgagggc cccggcgagc
    26161 ccttccgcct cgggcgcggc cggctcctgc gcccgcccgg cgcgcggcgc cagggtcgtg
    26221 gcgaggagga gcaagccgag gagagcggcg gggcggcggg gagagagcac gtcaccgtcc
    26281 cccgtcgttc cacccgggaa ggctccggcc ggagggaacg ggagcgcagg cgttgccgcc
    26341 cgtcgtgctc cgggcggccg ggcagccgga gcacgcccct ccgcaggcgc cgggccgtcg
    26401 gcggcggaag agacgaaggg cggcccagag cgcggcggcg gcgacgatcg tcacggtgat
    26461 gagggtgtcc acgcgggagt tctccttcag aggaagaggc gcccgacctg gaagacggtg
    26521 aacgaaacga gccaggccac ggccgtcagg ccgaagagct ggaggaaggc ccagcgccag
    26581 cggcccgact cgcgccgcgt cacggcgatc gtcgccatgc agggggcgga gatgaggagg
    26641 aagaggatca ggctgatgcc gacgagcggc gcgtagtcgc gggcgaggcg ggcgcggagg
    26701 ccggcggccc cctcctcggc gtcgccgatg gcgtagacga tccccatctg cgcgacgaag
    26761 acctccttcg ccgcgaacgc gccgatcatc gccgtcacca tcttccagtc gaagccgagc
    26821 ggggcgaaga cgggctcgag gaggcggccg gcgcgacccg cgacggagac cgacagctcc
    26881 tcggcggcgc gggcgttcgc gaggtcggac tcggggatcg cctcgccggc ggcgaggcgc
    26941 gcgtcgacgg cgtaggacga cggcttcggg aacgacgtcg ccgcccagag gaggatcgag
    27001 atgccgagga tgacggtccc cgccttgcga aggtagaggc cgccccggtc gagcatcttc
    27061 aggacgacgc tcttcagggt cggtatccgg tacggcggca gctccatgac gaacggggcg
    27121 tcctcgccgg gcaggatcgt ccggcggagg aggagcgcga ggaggagggc gagcgcgatc
    27181 ccggaggcgt agatgagcca gagcgccggc gctctccacg ccggggggaa gaaggccggg
    27241 acgaggagca tccagatcgg caggcgcgcc ccgcacgaca tgagcggcag gacgagcatc
    27301 gtcgtcagcc ggtcgcgctc gttctcgagc gtccgggtcg cgaggatgcc ggggacggtg
    27361 cagccgaagc cggtcatcat cggcaggaag ctcttcccgt ggaggccgaa gcggtgcatc
    27421 gcccggtcgg tcaggaacgc ggcgcgggcc atgtaccccg aatcctcgag gagggcgagg
    27481 ccgagaaaga ggaggaggat gttcgggagg aagacgagca ccccgccgac cccggcgatg
    27541 acgccgtcga cgaggagaga gcggaggacc gactccgacc ccgcgggcca gaggccggag
    27601 acggcgctcc cgaggaggcc gacgcccgac tcgatccacc ccatcggcgc ctctccgacg
    27661 gtgaacgtga gccagaagat cccgtagagg acggccgcga agatcggaag gccgagccag
    27721 cggtgggcga cgacgtcgtc gagccggtcg gagaggacgc gcgcgtcgat gcggggtggg
    27781 ggcgtccgcg tctcgcgcag gagtccgtcg acgaagccga aggtcctctc ggcgacgaag
    27841 accgagatcg ctctctcgtg cttcgcctcg agccgggcgc gctgccggcg cgcctcggcc
    27901 gcggccttct cgcggccggc cggtccttcg acccgcggtc ccggctcgcc cgcctcgagg
    27961 aggagcctga gcgcggccca ctcgcgctgc cggggggcga cctcgcccgg gaggagcggt
    28021 gcgatcgcgg agagcgcccc gtcgagctcc tcgccgagga cgacccgatg gcgggggaga
    28081 ggcgcggcgg cggcgcggcg gatcgcctcg cggagcgcgt cgagcccctc gccggtgcgg
    28141 gccacggtct cgacgacggg gaggccgagg agcttctcca tcagcgggac gtcgagcttc
    28201 tgccccgcgg ccctcgcctc gtcggccatg ttcaggcaga ggaccgggtt cgccccggcg
    28261 aggaggacct gcgagaggag gacgaggttc cgctggagct gcatcgagtc ggcgacgacg
    28321 acgacgacgt caggcccggt ggcgagctcg tcctcggcga ctcgctcctc cggggagaac
    28381 gcgctcaggc cgtaggtgcc agggaggtcg acgacctcga cggtcgcgtc cccgagcgag
    28441 aacgtcccgg tccgccgctc caccgtcacg cccgggtagt tcgcgacgtg ctgccgcgtt
    28501 cccgtgagag cgttgaagag cgacgtcttg ccgcagttcg ggttccccgc gagggcgatc
    28561 cggaagcggg cggccacggg aatcgtgctc tgcggtgcaa ggagtgttag agccatctaa
    28621 ttctctgtcg aaaaaagggt gaggtcacgc cccggcccgg acgaggacgc tcgaggcctc
    28681 ggcgcggcgg agagcgaggc gggccccctg gcagcggatc cagagcgggt ggccgccggg
    28741 gccggagcgc tccacgtcga ccttcgtctc cgggaggatc cccatgtcga ggagacgttg
    28801 tcggagggcg cccgtcgccg tgatctgcgt gacgacgccg ctctggccgg gcttcatggt
    28861 ggcgatgctc atggctttct ccttcggggc gaggctgcag cgggcgcagt cgggcgaggc
    28921 ggtcccgcac ggctccacgt cgccggggcg gctccccgtc gggcattgtc cgaatgccgc
    28981 cacgacggac gggcgcgtgc ccaggaactc gaagaacctc accatgcggt ccatcgcctc
    29041 gtccgtgagg gcgtgctcga gcgagcaggc ctgctcggac gcggcgtggg gagacatgcc
    29101 gaggacctcg gagaagaacc gcgtcaggag ccgatggcgc gtcaggacgc gccgcgcggc
    29161 ctcctcgccc cgctccgtca ggccgatgta ctcgcgccgg acgtagttca cgaaacccgc
    29221 ctcggagagc ttgcggagcg cgatcgagac cgtcgcggcc ttcacgccgc gcgccttcgc
    29281 gatctccttc acccgggcga agccatgctc ctgcacgagg aggtagatcg tctcgaggta
    29341 gtcctcgagg ggctcgctca tcgaggcctt cggggtgggg cgggcgagct ccattgttag
    29401 aagtctctaa cagaacggag ggggcgtcaa gaacgcgcgc cgccggcgtg cggatgccgg
    29461 gtggcccggg gccggcgccg ccacgtcacg gaccaccgcg tcgaggcgcc tttccgaagg
    29521 tctaagatgt cccgttgcgg cgggccgctg cgcggccgac cgcggggagg cgacggttgc
    29581 gaaggacctc cggccggttc cgggtcgacg gggcggcggg cggcgaggaa gcgccccgcc
    29641 ccgcagccgg ggtcgtccgg gaggcgtgcc cgtgagcccc ggctggcgga cggccctcgt
    29701 cacgacgctc cgggagcgct gccgggtctg cttcgcctgc gtccgcgaat gcccggccaa
    29761 ggcgatccgg atctccggga ggcaggcgga ggtgatcccg gaacgctgca tcgcctgcgg
    29821 caactgcgtg agggtctgca gccagggggc gaagcaggcg gtccgctcga tcgcccaggt
    29881 cgaggcgctc ctcgcctcgg gcacacccgt cgccgcctgc ctcgcgccga gcttcccggc
    29941 cgagttcccc gagctccccg tcccgcggct cgtcggggcg gtccgcgcgc tcggcttcgc
    30001 gttcgtccag gaggtcgcgt tcggcgccga cctcgtcgcc cgccgctacc gcgagctcct
    30061 cgccgagccc ggcacgcgct acatcgcgac gacctgtccc gccgtcgtga gctacgtcga
    30121 gcgctatcac ccgagcctcg tggagcttct ggcgcccgtc gcctctccga tggtcgcgac
    30181 ggcgcgggtc ctccggcgcc tccatggtcc ggaccttcac atcgtcttcg tcgggccctg
    30241 catcgcgaag aagggggagg accggtggcc cggcgccgac cccggcgtcg acgaggccat
    30301 cacgttcggg gagctccgcg agatgtggcg cgagaagggg atcgagcccg ccgacgcttc
    30361 gcccgaggag ttcgacccgc cgcgggccgg gagcggggcg atcttcccgg tgagccgcgg
    30421 gatgctccag gcggccgagg tcccggagga cctcacgctc ggcgaggtcg tctcggccga
    30481 cgggcggagc gcgttcgtcg acgcggtgaa ggagctcgag tccggggacc tcgacggggc
    30541 gacgaggctc ctcgagctcc tctgctgcaa cggctgcgtc atgggctcgg gaatgacctc
    30601 gaccgccccg ctctacaagc gccgcgccct cgtgacgcgc gcggcgcggg agcgcctccg
    30661 ggagctcgac cgtggccggt gggagggcga gatgagcgcg tatgcggacc tcgacctcgg
    30721 gcgctccttc ttcgcccgtc cggtcacggt cggggcggcc agcggggagg acgtcgcgga
    30781 gatcctccgg cggatgggaa agccgtcgcc ggccgacgag ctgaactgcg gagcgtgcgg
    30841 ctacgacacc tgccgcgagc acgcggccgc cgtccactcg ggcctcgccg agtcggagat
    30901 gtgcctcccc ttcacgatcg accagctccg gaacgcggtc cgcgagatcg cggtctcgca
    30961 cgaccagctc gcctcggccc agcaggcgct cctgcactcc gagcggctcg cgagcatggg
    31021 ccagctcgcc gccgggatcg cccacgaggt caacaacccc ctcggcgtcg tcctcctcta
    31081 ctcccacctc ctcctcgagg gggtcccgga gggctcctcg ctccggggag acctccagac
    31141 ggtcgccgcg gaagcggacc gctgcaagaa gatcgtctcg gggctcctcg acttcgcccg
    31201 acagaacaag gtctccctgt cggaggtcac cgtcgatgac ctcgtggcgc agagcatccg
    31261 gggagtcgtc ctgcccgaga gggtcgacgt cgaggtcgag cacgaggacc cggggctcgt
    31321 cgtcgagctg gaccgcgacc agatcgtcca ggtcgtcgcg aacctcgtcc gcaacgcggc
    31381 cgaggcgatg ccgcggggcg ggacggtcat cgtgaggacg cgggccgtgg ggagctccct
    31441 cgtcctctcg gtcaccgacg gcggcacggg aatctcgaag gagaacctct cccgggtctt
    31501 cgagccgttc ttcacgacga agccgatcgg gaaggggacg ggactcggcc tgccggtgag
    31561 ctacgggatc gtgaagatgc atcgcggaga catcgacgtc gtctcgaacg ccgaccccgc
    31621 cgccgggccg acggggaccg tcctgaccgt gaggctcccg cttcgcggga cgcggggcgc
    31681 ctgagcgtga gcggcacgct gaagatcctg gtcgtcgacg acgagccggg gatccggctg
    31741 gcggtgtcga gggtcctcga ggggcatcgg acgacgctcc ccgacgagtc gacggagacg
    31801 tcgttcgagg tcgcgcaggc cggcaccggc gaggaggcgc tcgccgcctg cacggcggga
    31861 gcccccgaca tcctcctcct cgactacaag ctcccgggcc tctccggcct cgacgtcctc
    31921 gagcggctcg gcccggcgac ggcggagacg atcgtcgtca tgatcacggc ctacgcgacg
    31981 atcgagacgg ccgtgacggc gacgcggcgg ggcgccttcg acttcctcgc gaagcccttc
    32041 actcctgcgg agctccggac gacggtgagc cgcgccgcga cccatctcgc cctccagcgg
    32101 cgggcgaagc gcctcgccga ggagaagcgg caggcgcggt accagcttct ctcggtcctg
    32161 ttccacgagc tgaagtcgcc gctcgcggcc gtggggggct acctcgacgt cctccgggag
    32221 gggtttctcc cgcccggctc ggcgcagtac gacgaggtcc tcgagcggag ccgcgcccgg
    32281 ctcgaaggga tgcggcgcct cgtcgtcgac ctcctcgacc tgacccgtct cgagtcgggg
    32341 cagaggaagc gcgagccggt cgagctcgac gcgcgggaca tcgtcctccg ggccgtggag
    32401 acggcacagc cggcggccgc ggcacgtcgg atcgagatct tcgtcgacgc gcccgaggcg
    32461 gcgccgctcg tggccgaccc gggggagctc gagatcgtcc tgaacaacct cctttcgaac
    32521 gcagtgaagt acaaccgcga aggcggccgg gtggacgtga ggctcgcgag ggacgccggc
    32581 ggcgtccgcc tcgtcgtcgc cgacaccggc atcgggatga cggcgcagga ggtcggacga
    32641 ctgttcgggg aattcgtccg gatccggaac gagaagacgc gcgacatccc cgggagcggc
    32701 ctcggcctgt cgatcatgaa gaaggtcgtc gagctcgccg ggggcgaggt gagcgtcgaa
    32761 agcgagcccg ggaaggggac gacgttcacg gtccggctcc cgtccggagc gcttcctccg
    32821 gttctcacag ctccgcacgt cgattgagga cgcggcgccg gagcgacccg tcgggttcct
    32881 gatcgaccgc gtatacagac ccggaagcgg cctgtttcca atgacttccg tgtcacggac
    32941 cgcatctgct ggtgataggc tgctcactcc atgagttggg gagctggccg tttcggttcg
    33001 gccgtggcga cgacggcgcc gtctccgatg cacctttctt cgacagctcg aatcccggga
    33061 ttcggacaag gaggtcacga tggcgaggat cctgatcgtc gatgacgaca aggacatcgt
    33121 cgaagcctgc cgcctcttcc tcgagagaga ggggcacacc gtagcagccg cctacagccg
    33181 gggcggcggg atgagggccg tctcggactg gcctccagac ctcctcatcc tcgacatcat
    33241 gatggacgag ccggacgacg ggatccgcat ggcccaggag cttcgccgct cgggctggac
    33301 gacgccgatc gtgatgctca cgagcatcag cagggttacc ggcctcggct accgcgccga
    33361 cccggacgtc ctccccgtcg acgcgttcct cgagaagccc gtgcgtcccg ccgtcctctc
    33421 gaagacggtg gccgatctgc tcgctcgcgc gggcggaagg gaggagagcc atgctggtca
    33481 cggaacggct ccggctgcac gatgacatcg gggcctgggt cgccaagcac ggcgccacgc
    33541 gcgccgcgct gatccccgtc ctccaggaga tccagcggag ctacaacgag atctccgagt
    33601 acgcgatgca ggaggtagcc gaccgcctcg acatccaccc ggtggaggtc tacagcgtcg
    33661 tcagcttcta cagcttcctc gaggcgaagg cccgcggccg cttcgtcatc cgcctctgcc
    33721 gcacggtctc ctgtgacatg accgggaagg accgggtcgc gcgccagctc gagaacgacc
    33781 tcggcatccg gttcggcgag acgacccgcg acgggaactt ctcgctcgag tgggcgaact
    33841 gcctcgggat gtgcgaccag ggtccggcgc tcctcatcaa cgaccaggtc ttcacgtcgg
    33901 tgacgccaga gacggtccac gacctgatcc aggcgtgccg caagagcttc ggcccgctcg
    33961 ccgtccaggg gcagaagggg aggacgcact gatgagcgcc gtgaagaagg gcctcgtcac
    34021 gttcgccgcg gccgttccgg aagagggcct gaaggccgcg ctcgcgcgcc cgcggtccga
    34081 ggtcatcgcg gaggtcaccg cctcgaagct gcgagggcgg ggaggggcgg gcttcccgac
    34141 gggcgtgaag tggaacctcg ccgccgcggc gaccgccccg gcaaagtggg tcgtctgcaa
    34201 cgcggacgaa ggggagccgg gcacgttcaa ggaccgcgtg atcctcaccg aggcgcccga
    34261 cctcgtcctc gaggggatga cgatcgcggc ctgggcgatc ggggccgcgc gcggaatcct
    34321 ctacctgcgc ggcgagtacg cctacctgcg cgactctctc gagaaggtcc tcgcgcggag
    34381 gagggagaag gggctcctcg ggacggggat cctcgggagc ccgctctcct tcgacgtcga
    34441 gatccggatg ggctcgggcg cctacgtctg cggcgaggag accgccctca tcgagtcgct
    34501 cgaggggcac cgcggcgagc cgcggaaccg cccgcccttc ccggtcgact gcggctaccg
    34561 gtcggagccg acgatcgtga acaacgtgga gacgttcgcc tgcgccgcca tggtcctcgc
    34621 gaagggggcg gagtggttcc gcggcatcgg gacggaggtc tcgaccggcc ccaagctctt
    34681 cagcgtctcg ggcgactgcc gcgagccggg cgtctgggag ttcccgctcg ggatcaccat
    34741 cgccgggctc ctcgccgcgg tcggcgggga aggcgcgaag gccgtgcagg tcggcggcgc
    34801 ctcgggccgc tgcgtgccgg ccgcggagtt cgggcggacg ctcgccttcg agggggtccc
    34861 gacgggcggc tcgatcatcg tcttcgggcc ccggcgcgac atgctccacg tggccgagaa
    34921 cttcctcgag ttcttcgtcg acgagtcgtg cgggcagtgc accccctgcc gcgaggggaa
    34981 cgcgaagctc ctcgaggggg tgaggatgct ccgcgagggg cgctgctcgt gggcctacct
    35041 ccaggagctc cagaagctcg gagaggtcat gcagctcgcc tccaagtgcg gtctcgggca
    35101 gtcgagcccg aacgccttcc tctccatcgt cgagcacttc cggccggaga tcatgggccg
    35161 gacggccggc gggaacgcct gaggagcaga gccgatgtcg agccgcatcg aacgcaccgc
    35221 ctccatctcc cgcgcgccgg tcagccagcg cggccacgtc gtcccggtct ccgccgaccc
    35281 cgaggcgatc ggcgggaccg tctccgtcac gatcgacgga atcgaggcga aggtcccgct
    35341 cgggacgacg atcctcgacg ccgcccgcgc cctcgggctg aagatcccga cgctctgcca
    35401 ccaccccgac ctggaggtcg cgggcgtctg ccgcatctgc gtcgtcgagg tcgagggcca
    35461 gcggaccctc caggccgcct gcgcctttcc gatcacctcg ccgatccggg tccagaccca
    35521 cacccgcaag gtgcggcagg cccggcggcg gatcctcgac ctgatgctca gcaagcacgt
    35581 cggggagtgc tacacctgtt tccggaacct gaactgcgag ctccagaccc tcgcgaagga
    35641 gtacggcgtc gacttcttcc gcttcggcca cgaggcggcg cgccggcacc aggtcgacga
    35701 ctcgtcctac tcggtccggc gggacatgga caagtgcatc ctctgccgcc gctgcgtgag
    35761 gacctgcatc gacctgcagg aggtcggcgt cctcgaggcc gtggggcgga gcgacggcac
    35821 gcggatcgcg accttcctcg acaagccgct gaacgacgtc gtctgcatca actgcggcca
    35881 gtgcatcaac cgctgcccga cggcggccct cggtgcgatg gacccgacgg acgacgtctg
    35941 gatcgacatc aacgacccga agaagcacgt cgtcatccag acggcgcccg ccccgcgcgc
    36001 cgcgatcggc gagtgcttcg gcctcccgcc cgggacgccg ctcacgttcg agatgaacac
    36061 ggcgatgaag acgatcgggt tcgaccgcgt cttcgacacg aacttcacgg ccgacctgac
    36121 gatcatcgag gaggggacgg agctcctcct ccggctccac gaggcgctcg tccagaagaa
    36181 ggacgtggcc ctgccgcaat tcacgagctg ctcgcccgga tgggtgaagt acctcgagca
    36241 cttctacccg gagatgatcc cgaacctctc gacggccaag agcccgcagc agatgttcgg
    36301 ggcgctcatc aagacgttct acgcgcagaa ggcggggatc gacccggcct cgatcgtcgc
    36361 ctgcgccctc atgccctgct cggcgaagaa gtacgagtgc aaccgtccgg agatgtgcga
    36421 ctccggccac aaggacgtcg actacgggct cgtcacccgc gagctcggcc agatgttcca
    36481 cgaggcgggg atcgacctgc cgaagatgcc gaagggggcg ttcgacgacc cgttcggcac
    36541 cgcgaccggg tcgggcgtca tcttcggggc gaccggcggc gtcatggagg cggcgatccg
    36601 gacggtcgtc gagctcgtga cgggcctgaa gatcgaggac gtcttcgccc acgccgacgt
    36661 cctcccggtc cgcggcttcg acggggtccg ctacgtggag ctcccgatcc cggcgatcgg
    36721 cgaggtcccc gacctcctga aaatgaacaa gggcctcccg ttctcggact ggaagtggct
    36781 cgagggggcc gtcctgaagg tcgccgtctg ccacggtacc gcgaacgcga aacgggtcgt
    36841 ggaggacatc aaggcgggag ggaccttctc gaagtgccac ttcatcgagt tcatggcgtg
    36901 cccgggcggc tgcctcggcg gcgggggaat gccgatcccg acctcgccgg agatccgggc
    36961 ggcgcgcgcg aaggcgatct acgccgagga cgcgagctac gagatccgca aagcccacga
    37021 gaaccccgcg gtgatccgtc tctaccagga gttcctgacg gacggcccct gcggggagaa
    37081 gagccatcac ctcctccaca cgcactacac gccccgcggg aagtacatcg cctgacccgc
    37141 gggaggacga ccccgacccc ggtccggcgg agcccgcctg gcgggctccg ccggagggcg
    37201 ggccgatgag ccgcagcgga gcgcccccac cgcccttcct cgcgccgcgc gaggcgaagg
    37261 tcctcgacct cgtcaaccgg aaggtcgccg ccgggcggag cctcgaggag gtcctcgact
    37321 tcctcttccg cgccacgcgt gacgtctgtc cctgcgaccg gatcggcgtc gcgttcctcg
    37381 aggaggacgg gcagcgcgtc gtggcgcgga gggccgtcgc cgactacgag ccggtcctcc
    37441 tcggcgccgg ctggtcggag gacctcgccg cgacctcgct cgggccggtc ctcgcgtcgg
    37501 agatgccccg cgtcctcgac gacctccccg cctacctctc ggcgcgtccg tcgagccgcg
    37561 cctcccgcct cctcgtcgac gagggggtcc gctccagcct gacggcccgg ctcgaggtcg
    37621 agggacgcgt cttcggcttc ctcttccgaa gcgcgcggag agtgcgtgct tacgatgccc
    37681 gccaggcgtt cctccacctc gcggtcagcg agaggctcgc ccaggcggtc gagaaggcgt
    37741 ggctcctcga ccgcctcgcc gaggcgagcg cgggctacgt cgagatgctc ggcttcgtca
    37801 gccacgagct gaaggcgccg ctcgcctcgc tcgtgatgga cggcgaggcc gttctcggcg
    37861 gctacgccgg ggacgtggac gaggcggcgc gcgccgccgt ggaacgaatg acgaggaagg
    37921 cgtcctacct cgggcggctc gtgaaggagt acctcgacct cgcgcgcgtc gaggagcacg
    37981 acctcgtcgc ccggcgcgtc ccgtgcgatt tcgtcgccga cgtcgtcgag ccggctctcg
    38041 acgtcgtccg taccctcctc gaggagcgga gcggccgcct cgttcgggac ctcgcttccg
    38101 gcccgctccc ggtctccgtc gaccccgagc tgatgaccgt cgtcctcgtg aacctcctcg
    38161 ggaacgccgc gaagtacgcg cgggacgggg gcgaggtccg cctctctgcg cggtgcgacg
    38221 gggagtccct ccgggtcgcc gtgcggaacg aggggccggg cttccgtccc gaggaacgcg
    38281 ctcgcctctt ccggcgcttt tcgcgcctcg cggacccgga gctcgccggc cggcccggct
    38341 cgggcgtcgg cctctacacc tgccggagga tcgtcgctct ccacggcggc cggatcgacg
    38401 ccgagtccga gcccgggtgc tgggccgagt tccgcttcga ggtcccggcg tcgctgcgga
    38461 ccgtcgaggc cggcgaggag aggtcggagc ccgtaagatc gcggccatga acccggagcg
    38521 cgtgcggcgg tttccacggc cgccgcttcg atgtcctctc gggggtccgt cgacgaggcc
    38581 gtgggccacg gccccgtcgg gcaccgtgct cgctctcctc ctcgcgggcg agaagcggac
    38641 cggctccgac gacggctacc actcgggcta cccgatgccg gaagcgtcgt tcagcggcgt
    38701 ggcgagcctg cgtttctgat ggccgcgacg gctgaggcag cgcgtcgggc cgcgttcggg
    38761 gcgctcgcgg cgacgtggga cgcggccaag ccgcgcgagg cgatcgaggc cggagtcgcg
    38821 cgcgggctcg acctcctcgg cgacgtccgc ggccttcgcg ccatcgacct cggagccggg
    38881 ccgggacgtc tggagccgtt tctcctgccg aggctcgggg gcggacacgc cgtggcggtc
    38941 gatttcgctc cggagatggt cgtccgcggc gcgctggagg tccgcgatcc gcgcgtgacg
    39001 tggctctgcc gcgacgtcct cgagaccggg ctcccggacg cctgtgccga cctcgtcctc
    39061 tgtttcaacg cctgggccca ctttccggac ggtcccgggg tcctgcgcga ggcgcgccgg
    39121 tggctcaagc cggggggacg catcctcctc tggcacgacc tgggacggga gaggctggcc
    39181 gaggtgcacc ggcgggcggg acacgcggtg tcgtgcgacc tgcttcctcc ggtcgaggcg
    39241 ctggccttcg ccgcgcggaa cgcggggttt gccgtcgaac gggccgagga ggacgaccgc
    39301 tcctggactc tcctggcctg cagcgtcggt tgacgcggcg tcaaccgact ccgtccggcg
    39361 gcctcgcgag ctccgccgtg ccgcagtccc gcttcggctc gccggcgtgc gggcagggga
    39421 ggacctcgac gttcacatga gcgaggtcga ggccttcggg gaggagggca cggtagccgt
    39481 cgggagagag cgggtcgtca ctgacgacgg agacgatcgc ggaccagccg tcgggggaga
    39541 tgcgccagac gtgcaggtcg gagacgcgat cgccggggcg cgcctcgatc gctccccgga
    39601 ttgacgagag gagcggctcg ggcgcctgcc ggtcgaggag gacgtgtccg ctctcccgca
    39661 ggaggccgac cgaccaacgg gtgatgacgg cggcgccgac gatcccgatc agcgggtcga
    39721 ggaagcgcca gccgaggagc gcgccgccga ggagggcgcc gatcgccagg agcgaggtga
    39781 cggcgtcggc caggacgtgc aggtaggcgc tccggaggtt gtggtcgtga tcgtgaccat
    39841 gatcgtgggc gtgatcggcc gggcgcgcgg acggctcgcc gtcgtggccg tcgtggggtc
    39901 tctcgccccg cgtcaggacg agggcgctca cggcgttcac ggcgagcccg aggacggcga
    39961 cggcgatcgc ctgggagaac gcgaccggga cgggagagag gagccgctcg acgctctccc
    40021 aggccatcag gagcgcgaag cccgcgagga ggacggcgcc ggcgaagccg gcgagcgcgt
    40081 tcatcttccc cgcgccgaag ctgaaccgcg ggtcggtcgc gtgccgtctc acccaggcgt
    40141 acgcgaacgc cgagatgccg agggccgcgg cgtgcgagcc catgtggagg ccgtccgcca
    40201 ggagcgccat cgagccgaac ttcaggccgg cgccgatctc gacgagcatc gtgacggcgg
    40261 tgagcgcgat gacgatccgg gtcccgcgct cgccgggccg cggacggtcc tggccgaaga
    40321 cgtgctcgtg ccgccggccg gtggcgcagg cctcgtgggc cgcggaggcc gggtcggccg
    40381 gcgtcaccgc ggtcggagcc tcgcgacgac cgggtcgtgg tcgctcgtcc gcttctcgtc
    40441 ggagcagtcg gagttcaggt ggaggatctc gatctcggcc tcccgggcga gggcgggcga
    40501 gacgacgacg tggtcgagga cctgcgaggc gccctcgaag ttgaacgtgt aacggtttgc
    40561 ggccgggacc ctctcgacga ggttctcgag gggcggggcc gagagcgccc tcaccgcctc
    40621 ggagtgctcg aagtcgttca ggtcgccgag aaggatgatg cgagccttcg ggtcgacggc
    40681 gaggaggctc tgggcgaagg cccggatctc ccgcgcctgg gcgaggcggc gcgcgacggt
    40741 tggcgccctc gggggctgga cggagccgaa ggcgcggtcg tcgtcggact tggacgaaag
    40801 gtggttcgcg acgacgaaga gcttcttccc gcggaagagg agctcgacgg cgagcgagcg
    40861 gcgcacccct tcgccgtccg tcggcgagaa cgcctccgag gcgggcgcga cccgccccgg
    40921 gctgagcgtc aggcggaggt ccttcccggt cccctccggc gccgtcgcgt cgagggggcc
    40981 ggccgacccg cggcgaacga gcgtgacccg cgcggggtcc aggaggagcg cgacccggat
    41041 gttccctccc ggctggcccc cttcgcgccc ctcgatcgga tcgatccaga cggcttcgta
    41101 gcgcggccct cccgcggcca cgaccgcgtc gacgagcgac tcgagagtcc gcttcgacgt
    41161 gacgacgccg tcgcccttgc cggccgggcc gctgtcgtcc tgcacctcct cgagggcgac
    41221 gacgtcgggg ccgagcatcg ccttcgcgat ccgctcgccg atccgggtga aacgctcggc
    41281 cggaccggcc gcggagaggt tctcgacgtt gaacgacgcg acggagagcc ggcgccgatc
    41341 gccgcggagc gacgtcaccg cgtcgcagcc gcccgcggcc gccgtcgtgg ccggagcgac
    41401 gagcggtctg acgcggtagg tgctccaggc gtagtccacg atcccgacga agggcgcgac
    41461 gacgcgcgcg ccgacgtcga ggatcgccgg atctccggcg aacgggcgcg agacgaagac
    41521 ccggtcgagc gacggacctc tctccgggag gaggactccg ccgaccgcgg tccgggcgac
    41581 ctccggcgag ccgtccggcc ggaggacgaa gtctcctcgt cgcgaggagg ggccgacgac
    41641 ggcgccggcc ggaacttcga cccgcatcgc ttcgaggctc tcccagaagt cgagcgcgtc
    41701 ggaggcgggc tcgaagcgcg cgagcgcgtc gtcgtcgacg acggacggca gcggcccgcg
    41761 cgcgaacggg cgggctggtg gcgggagcgc gaccggaccc ccgagcggga cgacggcgct
    41821 cgccgatagg cgtgtcacgg agaggccctt cggctgctcc gtctcctcga cgcgtccgga
    41881 gacggagacg gcccgtcccg gctcgagcga cgcggccgcg gaccacgaga cgaagagccc
    41941 ctccgaggtc gacgcgtcgt cgtcgggccg gagcgactcg aggaagaagc ccgggttccg
    42001 cgggtcgcgc tcgactgcgg tgacgacgcc cgggacgtcc cagatccggc tcccctcgat
    42061 gggggagcgg tggccggcgc cctggatgcg cgccacggac accgggccgt ggtcgacggc
    42121 gaagacgaga aacgtcgtcg actcctgctc cgggttgaag ttgtcgtcgc tcgcgacgac
    42181 gagcgaggcg cggccgtcgg ggagcgtcgg gccgaacgcg aggccctcga agttctcgag
    42241 cgcgacgccg gtctccgcga ggtcgaggag gagcgtcctg cgagccggga cgtactcggc
    42301 gccggagagg gactcgagcg aggagacgtc ggtcgcccct tggagcgaga cgtcccagat
    42361 ccgcgcggcg tttccggtcc cgtcgatgaa ctgcctctcg aggacgagga ggcggtccgg
    42421 gccgagcggg aggaggtccg agaggccgtt gacgcggaag aggccttcgt tcaggggagg
    42481 aggcgacacg gggtcgacga cgtagacgaa ctcggtgggg ggaccgcccg aggcgagatc
    42541 gaatcggagg aggcgcgcgg ggctcggcgt cttcacgtcg gcgggcgcgc cgtcctgctc
    42601 gagcgcgttc tcgaggccgg cgaagaggta acgaccgtcc ggcgtcgccg ccagtccctc
    42661 gaagccgagg ttgtctcgaa cgcctcgacc cggagccggg aggagcttcg cgggaagggc
    42721 gagctcgcga cggaggcggc cgtccggagc gtgctcggtg atcgccgcgg gaatgccgtg
    42781 tcgtgcgatc gcctccgtcg agacgaggag ccgccccggg aggagcgcga gcccctcggt
    42841 gtcgatccgg gacgggacga agggccgtcc cttcgcgtcg gtcaggagcg tcatcccttc
    42901 gacggaggcc gtcgccgtga aggggaagcg cccgtcgcgc gcgtccgcga cgtccaggcg
    42961 gatgcgatag acccggaccg gtccgaagcg gccgcggtcg tcggagatcg cgaggaaccg
    43021 gccgctctcc gcgtcccagg cgaggcccga gagccctccg acacgtgtct tctcgtactc
    43081 gagatcgcga ggaaggaccg cgcggccgac gagggagaga gccgtgaagg gtgcgggcgc
    43141 gttcggtggg atggcgcggg tcggcgaagc ggcgagcaga gcggcgaggg cgacggtcga
    43201 tatgcgcacg gggatcgaac ctcctccgag aaggcgcagc atagtttgtc gggttcgtcg
    43261 cggaaaggac ccgggggagg ggcgccggtc gtccggcggg ttccggcgct cccgggcttc
    43321 ggccctcagt ccttcccgaa gaagatcgcg tcgagccggt tctcgacgac gtcgtcggcc
    43381 gagaagccga agatctggag gtagtgctcg gccgaggcga cgagcgcctg ctgcgggacg
    43441 agacccacca tctcgacgcg gctgactcga gtgccgaatc gccggagctc cgtcttgacg
    43501 gcctcgagga tccggtagag cggcgtcgcc aggaagttgc cgacggtgat gttgaggagc
    43561 gcttcgttcc gggtccggtc gagggccggg tagaagtgga cgccggagaa cccggtcgcc
    43621 gggtcggaga ggacgtggga gacccactcg gccgcctcct cgctcgacgt cgcgaggtag
    43681 gtcttgaagt tcacgatcgg caggcgtgcc ccgatgatcg tcgccccctt gtcgggcggg
    43741 aagacgtcgg gaccgaagtc gggcttccac tccggctggt tcatcttcgc cgcgaagccc
    43801 tcgtactccc cctcgcggat gctctcgatg tcacgccgcg ccttcacgcg cgccgactcg
    43861 gcgaagaggt agaccgggag cgcgaaacgc ccggcgacct cctcggcgaa ctggacggac
    43921 cagtcgacga ccgtcgggag cggcgcgtcg cgcagggcga cgaagggaaa gacgtcgacc
    43981 gccccgatgc gggggtactc cccctcgtgc tggcgcatgt cgatcttccc gagcgcctcc
    44041 tcgtagagga ggtagccgcc ctggaagatc gcctccttcg atcctgtgaa ggagaagatc
    44101 gtccggttcc ggacctggtc catcgagacg tcgaggacga cgaggcccgg gacggtctcg
    44161 agccgctcct ggaccttcgc gacgaacgct tcgtcccggc cctcgcagat gttcgggacg
    44221 acggagatga tctgggacat cgggtcctcc tcaggatcgc gccggctggc gcggcgttcg
    44281 ggtctcgtgg ggcgcggtgc gcggactcat gtcagaagag gccgaggaag acgccggccg
    44341 cgagggccga gccgatgacg ccggccacgt tcggccccat ggcgtgctgg aggaggaagt
    44401 tccccgggtc ctcggcctgc cccaccatgt gggcgacgcg ggccgagtcg gggacggccg
    44461 agactccggc cgccccgatg agggggttca ccttctcctt cgcgaagacg ttcatgagct
    44521 tcgcgaagag gacgccgccc gccgtcgccg tcccgaaagc gacgcagccg aggacgaaga
    44581 tgaggatcga ctggggtttc aggaagactc cggccgaggt ggaggcgccg acggcgatgc
    44641 cgaggaggat cgtgcaggtg tccaggatcg cgctcgaggc cgacttcgcg agccgggtcg
    44701 tgacgcccga ctccttcagg aggttcccga agaagagcat cccgaggagc gggaggccgc
    44761 ccggggcgag gagcgtcgtc acgaggagcc ccatgatcgg gaaggcgatc ttctcgaacc
    44821 tcgagacctt ccggcccggc ttcatccgga tgagcctctc ctccttcgtc gtcaggagct
    44881 tcatgatcgg cggctggatg atcgggacca tcgccatgta gctgtaggcc gagatggcga
    44941 tcgggccgat gagcgccgga gcgaggcggc tcgccgtgaa gatcgccgtc gggccgtccg
    45001 cgccgccgat gatcccgatc gaggcggcgc cctgcggcga gaagccgagc cagagggcac
    45061 cgaggaacgt catgaagatg ccgatctggg ccgcggcgcc gaggaggagg ctcttcgggt
    45121 tcgagaggag cgccgagaag tcggtcagcg cgccgatccc gaggaagatg aggggcggga
    45181 agacgcctgc cttcacgccg aagtagatga tcccgaggac gctgttgccg ccggagttga
    45241 agacgtactc gtgcgagacg gggtcgatca tgtacagcga gacgctgttg aagatcgaga
    45301 ggggcatcgg gatgttcccg acgaggatcc cgaagccgat cgggacgagg agcagcggct
    45361 cgtagtcctt cgcgatcgcg aggtagatga agaggagccc gacgaggacc atgacgacgt
    45421 tgccggccgt caggttcgcg aagccgctcc cctggacgac ctgggcgagg tagccggtga
    45481 tgtcgccggc gacgacgtcc ttcgcgaaga agccctgctc gaactgggtg acgtagccga
    45541 ggctctcggc cgcgccgacc gtcacgacgg tgtagatcgc cgcgacgcgg ccggaggcgt
    45601 cgaagagccg cccctttgcg gcccgcttga gcgtgtgggg gtcgaggtgg tcggtcccct
    45661 tgtcgatccg gaagtagagc ccgttcggct cgaccttgta ggggaggaag ccgatcttca
    45721 gcgccgggac cgcgacgtgg tcgacgtcgg cgagggactt cccctcggcg tagccggtga
    45781 ccttcacgac ggtctcgaag tcgtcggcgt gaacggttgc gaacgtcccc acctcgcgct
    45841 cgagggcgtc gcggaggagg ccggtcgtcg ccttctctcc gaggtagtcg ttcacgaggc
    45901 cgtcctgcgt ccccttgtgg atccgcaggt attccgccgt ggcgtcgaac gcgacgccga
    45961 acgtcgggcg ggggtcggcc cacgcggcgg gggagacgag gaggaggagg gccgggagga
    46021 ggcggagccg catcgacgtc cgcgcctcag agggtcgtca tcttcgggcg ggcgacgtcg
    46081 acgaggaggt ccccctcgcc gatggagtcg ccctcgcgga cgtagacctt cgcgaccgtc
    46141 ccggcgtacg gcgtcgtgac ggcgttctcc atcttcatcg cctccatcac gaggagcgtc
    46201 tggccggcgg cgacggtgtc cccctccttc gctttgatcg agaggacgag tccgggcatc
    46261 ggggcgacga tcccgccctc gccgcgggcg gacgcggcgg gacgcggggc ggcggcgggc
    46321 gcgggcgcgg ccggtcggac gccggaagtg gcggcgggcc ggggcgtcgg tgccgcggac
    46381 ggccgggcgg cgtcgaccgg gatgacccgg cggccgagct ggaggatctc gacctcgtac
    46441 tccttgccgt cgacgaggat gagggcgtgc tccggggtga ggtccttgac ctcggcctgg
    46501 tactcgcggc cgccgatgcg caggacgtgc tgggtcatgg cttcctcttc tcgcgctcag
    46561 ggccgggggg ccgagcgccg gatggtgacg cgggaaccgg ggcggttcag gtagagcttc
    46621 ctctccacct ggaggaccgt tgagatgacg gcgaggacct cggccgggat cggctctccc
    46681 gccggcgcgg cggcggggac aggctcgggg acgggcgcgg gagcaggggc accgtggccg
    46741 tgcccctcct cgccccaggc gatccgcgcc gacagccggc cgaagagccg gatgaaggcg
    46801 atgcacgcca cgaggccgat gaagacgacg gccatcccga gcgcggtgat cgtcagggct
    46861 tccagctgcg acatcgacgc tcctcagagg ggaatgttcg tgtgccggcg cgcggggttg
    46921 ccgtcgcgct tctcgccgag catctcgagc gccttgatga tgcggaagcg ggtcgtcgcc
    46981 ggcttgatga cgtcatcgat gtaacccttc ctcgcggcga cgtacggagt cgcgaagagg
    47041 ctcgcgtact tctccttcag ctcggcggcc ttcgcggcga cgtcctcggc ggcgtcgagc
    47101 tccttcttgt agatgatctc gacggcgccg tccggcccca tgacggcgat ctcggccgtc
    47161 ggccaggcgt agttcacgtc gccgcggagg tgcttgctgc tcatgacgca gtaggcgcct
    47221 ccataggcct tccggaggat gacggtgacc ttcgggacgg tcgcctccgc gaacgcgaag
    47281 aggagcttgg cgccgttccg gatgatcccg ccgtactcct gcgtcgtgcc gggcatgaag
    47341 ccggggacgt cctcgagcgt gacgagcggg atgttgaagg catcgcagaa gcggacgaag
    47401 cgcgccccct tgatggatgc cgcgttgtcg aggacgcccg cgaggacctt cggctggttc
    47461 gcgacgatcc cgacggagcg gccgttgaag cgggcgaaac cgacgacgag gttcgcggcc
    47521 cacgcctcgt ggacctccag gaactcgccg cagtcgacga tcgaccggat gacgtccttg
    47581 acgtcgtagg gctggttcgc gccgtccggc aggacggcgt tcagctcggg gacgggccgg
    47641 tcgatcgggt cctcgcacgt caggagcggc gccctctcct ggtagttctg cgggaagtag
    47701 ccgagcagcc gccggacgag cgcgagcgcc tcgtcctcgt tctccgcgac gaaatgcgcg
    47761 acgccgctct tgccggcgtg gacgtcggcc ccgccgagct gctcggtcgt caccgtctcc
    47821 cgcgtcacct gcttcacgac cttcggcccc gtcaggaaca tgtagctcgt gccgcgcgtc
    47881 atcgtcacga agtccgtgat cgccgggctg tagaccgcgc cgcccgcgca cgggccgagg
    47941 acgaggctga tctgcgggac gacgcccgag gcgaggacgt tcctgaggaa gatgtcggag
    48001 tagccggcga gcgcgtcgac gccctcctgg atccgggcgc cccccgagtc gttcaggccg
    48061 atgacggggg ccccgacctt cacggccagg tccatcacct tgcagatctt ctcggcgaag
    48121 gccttcgaca ggctcccgcc gaagatcgtg aagtcctggc tgaagacgaa gacgagacgg
    48181 ccgttgaccg tcccgtggcc ggtgatcacg ccgtccgtca gcggctggtc cttctccatg
    48241 ccgaagtcgg tgcagcggtg ggtgacgaac gtatcgaact cctcgaagct cccctcgtcg
    48301 aggagcttct cgattcgctc tcgggccgtg tacttgccct gctcgtgctg cttctggatg
    48361 cgcttctcgc cgccggcgcg gtacgcgttc tggcgcaggt gggcgagctc gaggatcctg
    48421 tgttcgatgc tcatttgagc tccgcgcggc cgcgtttcgt ttgcgtggct ccggggcgcc
    48481 tggggctgcg accgacgaaa ggtcatcgta gcattgcgtg cggggccgac gcgcgttcgg
    48541 gaatgcgacg ccggccgcga ctcccggctc gttccggacc gatccgggac ggattcggcg
    48601 atcgagagcg cctatgatcc gcggccggag tcgaaacgat gaagcgaaag agcctgaccg
    48661 tcgtcctcgc cgccacgctg cttctcctgg gcgtcccggt cctcgcgacc gagatcgacg
    48721 cggagacgga ggcgcgcgtc cccgctctca cggccttcca cgaggtcgtc tacccgctct
    48781 ggcacgaggc gtggccgaac aaggacgtcg acgccctcgt cgcgctcgcc ccgaaggtcg
    48841 acgccggcgt cgccgcgatc gcgaaggccg agctcccggg gatcctccgt gacaagaagg
    48901 cggcgtggga atcggccgtc gcgcgcctga aggcgatcgg cgccgagtac gccgccgcct
    48961 ccacgaagaa ggacgggacg gcgcttctcg ccgccgccga gagcctccac gcccagttcg
    49021 agaggatggt gcggatcatc cgtcccgtcc tcaaggagct ggaggcgttc cacgcgacgc
    49081 tctatgttct ctaccaccgc cagctcgacc cgttcgcgct cgaggccgtc acgcagtcgg
    49141 tccggacgct cgacgacaag atggtcccgc tcgccaccgc ggcgcttccc gagcggctga
    49201 aggcgaagga agaggcgttc ctcgccgcgc ggggcgccct ctccgcggcg gtcgacgagg
    49261 ccgtcgcgac gctcggccgg aaggacgaga aggagatccg cgccgcgatc gagctcctcc
    49321 acacgcgtta ccaggagctc gagaaggtct tcgactgagg tctcgcgagc ccgcggtgtg
    49381 acgggcggcg agcccgtctg ctgcccgcag gcgctggccg tcctcggcgt ccgacgccgc
    49441 cgtcaggacg tcggggacgg cagcgtcacg accttggtcg cgaacgcgtc gaggtgcgga
    49501 cgatgggtcg ccacgacgac gcccgtccct cgcttggcca gccagcggag cgttgcgaag
    49561 accgtctcct ccgcctcggc gtcgagggcc gccgtgggct cgtcgaggag gaggagcgag
    49621 gggcggatca ggaggacgcg agcgagcgcg acccgctgtc tctgcccggc cgacaggacg
    49681 acgcctcccg gccggaggag cgtgccgaga ccgtccggga gcgacgcgac gaggtcggga
    49741 gcgccggacg ccgcgagggc ctgccgcgca tcttcgtcgg aagcgtcggg cgcggcgagg
    49801 cggaggttct ccccgaggct ccctcggaag acgtgcggct cctgccggag ccagccgatc
    49861 gaacggccga ggcttcgtcg gagacactcc ggcgcctctc cgccgccggg cacgaggacc
    49921 tcgccggagc gtgcttcgag gaggccggcg cagatccgaa ggagcgtgct ctttccgctt
    49981 ccgctcgggc cgtggacgac gacgaggtcg ccgggtcccg cggagaggtg gaccgactcg
    50041 aggcggaagc ccgattccca ccggaagccg acgcccacca gctcgatccg gtcgacgtcc
    50101 cttccgatcg gcatcgccga caccggcgga gcggccgcga ggagccgccg gaagcggcgg
    50161 cggactccga ggaagacctg gaggtgcggg agggagcttc ccgcggcctg gacgtgtgcc
    50221 gaggagacac cgaggcagac gaggaaggtc gtcagcgacg gagagtccag ccggccggcg
    50281 gcgtggaggc cccaagccac cgaaaggacc acggccgcgg cgagggtgct cgagagggtc
    50341 acccgaaggg ggtgggcggc gcgcgcgaag acctcgcact gcgccgcgga gcggagcgcg
    50401 gcggccgcgc ccgtgaggcg tccgaggggc cccggcggcg tgccgagcgc gacgagctcc
    50461 tcgcgcccca cgaggaggtc ctcgagggcg tcgccgaggt cggacctggc cctgcgcgtc
    50521 tcttcgagcg cctcgggcat cgcggcgccg gctacctgcg aggcgagaac gagcgggacc
    50581 gccgcgacca ggaggaccgc cgcgaggatg ggggccgccc aggtcatcac gccgaggagc
    50641 gcgatgacga ggacgacgtt cgggaggaac gcgagaaccc cgtcgagcag ccccctttcg
    50701 aggagcgccg cctccgagaa gaacgccgag atcgtcgtcc cccgctctcc gtcggctccc
    50761 gacctgacgt cgaggaagct cgtggcaagg gcgcgtctcg ccctctctcc cgcctccagg
    50821 gcagcgcggg agaacagtac ctcccgcgcg aaggtcgccc cggcggcgac ggcgacgagg
    50881 ccgatcccgg cggcgaggtg cgcgaggagt ccggccggtg agggtgcggt gagcttctcg
    50941 acgagccgcg tcgtccagcc ggggaggagg aggtaggcgc ctcccgtgac gagcgtgaag
    51001 agggctcctc cggcgatcgc ccgcttcacg cgacgaccgc ctcgagctcg gcgaggatcc
    51061 tcccggcggc gccagccggt cccatcgccg agcgcaggcc gttcgcgagc cgcgctctgg
    51121 ccgcgccgtc ctggatctcg cgcgcgaggc cgaggagggc ggtgacgacc tcctccgtgc
    51181 cgaagtcccc gcggacctcc gggacgagcg gccgtccggc gcggaggttc gggagggcga
    51241 cgaggggtcg cctcgcgacg aacgttctga aggcggtggc gtgcagtccc ctgccgacga
    51301 acggcaggcg ccgcagctgg ccggcgagac ccgggatggg aacgagcgcc gggtcgtgcg
    51361 tcggaaggag ggcgacgagg gggagcccgg ctgctgcggc ctcgccggtg accgagccga
    51421 cgggtgcgag gccgaagtcg gcctcccgga gcgcgcgacc ggtgcggacg atcgggacgg
    51481 gaggaagccc ctcgacctcg agagtcccgc ccggtcccgg tgtcccgagg aggcgagccc
    51541 tggagccggg caggattccc ggcgaaccct ccagagccgc gaggaacgcc tcggccgtga
    51601 cgaggtccga gagcgtgacg aggaccccca gaccggggag agaccgcgac agggtcgagg
    51661 cgacgaggag ctggaacggc aggaagtgac gaacgaggag cgcccggcta cccgggaaca
    51721 gggcgaggac gagaccgtcg gcccggttct catccacgcg ctcccggctc gctcccacct
    51781 cggcccatgc gtcggtccgg aggtcgccga cggcccgggg accggcgacg ggcaggctcc
    51841 tctcgaagag gcggaagatg gctccgtcgg gctcgtggta ggcgacggcc gggcagccga
    51901 gtcgtctcgc gaggaggagg ctcagcgccg gctcgccgcc gatgtggagg agtacgtccg
    51961 tcggcgcgaa gctcgcgccg ggtcggcggc agaggaggcc gcggatcgcc tcggccggcg
    52021 gcacgacgga ggagacggtc gggagcgtcg cggccagctc gggctcccgg ccgctggcga
    52081 agcggcacgg ccagaggacg atctccgtcg tgaagcctcc gcgcgcctcc agccgtgcca
    52141 ggagcggggc ggcccaggcg aggagctcgc cgggaccgtt cgcggtgagg aggagcctca
    52201 ccgcggcctc gatctccggc cgagccccgg acgctggagt cgggccgcga ccgcgaggac
    52261 ctccgcgatc gagacgcccc atgcccggtt cacgaactcc ccctgctcgc cgaggacggc
    52321 cggcaggaac gtcgacggcg tctcgtcgag gatctgcgct ccgtccgcca cgccgagcgc
    52381 catcaggcgc tggggcaagg cgctctcgcc tcgacgggtc gtcgtgaccc cgtggatgca
    52441 ggtagctcct tcaccgaagg acgcgacgac cgtgtggagc aggaggccgg cgagctggcg
    52501 ggaccgcgcc aggggcagga cgttgaagtg gaggtgagcc gggtagcgat cgagcgggat
    52561 gcgcggcgcc tcgcgtcgcc cggggccggc gagccagcga aggagccgtc gtggccccgg
    52621 ctcacgggag gcgacggcca gcgccgcgag gatgcgcagt gcgcgaggca ccgagcgcct
    52681 ccggaagcgg gcggtgtccg gtgtcccgac cacgtagccg accacgcgct cctcctggcc
    52741 ccgctcgact gcgacgaagg cgtggcgcgg ctcgaggtcg aggtacggcc gcacgaacca
    52801 cgcggcgacg aaaccgggac aggggagctg cgccgctccg cgatcgcaga aggccgtctc
    52861 ctcgcagatc cggacgacgt cgtcccggtc ccgcgcctcg aacgggcgga ccgccgcctc
    52921 agtcgccaac gggaaggcct ttccggtatc gcacgccgcc ccagacgacg tcgggggaga
    52981 cggcgcgggc cgcgaggacg gccgcggccg tcgcgagagc gaacggctcg aagagaggcg
    53041 cgaccgctgc cgcccggagc tcacctcgtt cgaggcagag gaacgcgagc accccggcgc
    53101 cgaggagcca gcgggcggcg aggaacccga gtgcggcgcc tccggtggcc gcggcgacga
    53161 cggcaggtcc ggcgagcgac acgagggcga gcgcgatcgc gaggccgaga agaccgctct
    53221 ctccgcggct cgtccgccgg ccacgggcga cgatcgagga ccagcttgcc ggagcgggga
    53281 ccgtcacggc gctccgggcg tcgaaccgga cgcggaaccg ggcgcgctgc agggcccgga
    53341 cgaggaggag gtcctcggtc agggatggcc cgaggcgctc gaaccctccg accgcgaggt
    53401 acgcctcgcg acggaacgcg aggttccccg ccacgccgcc gctcgccgcg ccgagcgcgt
    53461 cgagagagga gcagaggaat tggtagacga cgccgtcgca cgtcatgagc gcggagagga
    53521 ggccgcggcc ctcgtagcgg actcgtccca cgaggacatc gacacggccc gaggagacct
    53581 cgtccgcacg cgcgagccag ccggacggca cccggccgtc cacgtcggtg aggtagatcg
    53641 cgcctccccg cgcggcgcgg attccctggt cgagcgaggt ctgcctcgtc gtcagccccg
    53701 ctgtcgggag cccctcgttt cggaggacga cgaggccgct gacgccggtc gcgcgcgccg
    53761 cttccgccgt gccgtcctcg ctccggtcgt cgacgaggag cacctccgaa ggcggtgcgg
    53821 ccgcgcgggt cgcgtcgatc gatgcgagac agcgcgagac cgtctccgcc gcatcgtgcg
    53881 cggtgacgac gatcgagacg tccggggcag cggcgccgct cacgctccac ctgcgatcgt
    53941 cgcgaggtcg accgggacga atccgccgct cgtcagggcc gcgaggaggt tccgcacctc
    54001 cgcgacggtc gacggcgacg tcgcgtcccg gggatgaagc gcgacccgca cgattcgcgc
    54061 cgagcggagg agcggcccga ggcgaagccg tcggacgagc cggttcgcgg cgacgacgat
    54121 gtggcgggtc ccgtggtcga agctgaggac gggggcggcg tgggtctggc cggaggaaag
    54181 aaggagcgcg cgtctcccga gggtcccttc gaagccgagc tccgcgagga ggccacgcac
    54241 gtgacgaggc tccctccagc gcggggcgca ccaccattcc acgcggcggc ccgtcgcctc
    54301 cccgaggagc agacggccgc ggcggatccg ctctcgcgcc tcctgccggc cgaggcgggc
    54361 gaactccgat cccatcgcgt cgccgtagag gaggcgctcg aacgccgatg ggggagcgtc
    54421 gtgggtgagg ccatggagga ccggggtgtc cgaaggcgtt acgagccgcg ccagcgcacg
    54481 tcgggaggcg cgtgcaccgc cccactccgg cgtgacgagg agagccggcc tgccggctcc
    54541 ggtctccgcc gcgagggagc gaagccggtc gatcgtctcc gcctcggcgg gacgggcgtc
    54601 gtggatctcg accaggagga gcctcttggg ggaggtcacg cgaggagctc ctcggcccgc
    54661 gcgacgagcg accggagcga cgtccccccg gccattcgcg cccggccggc gcgggacatc
    54721 gcctcgcgcc ttcgcgggtc ggagaggatc gtgaggatcg cgtcggcagc ggcttccggt
    54781 cggggcggca cgaccacctg ggcctcgccg aagagaggcc gcttcatcct cacgtagcga
    54841 gcgtcgaaca cgccgtcggt cggccaggtg accaggggaa cgccgagacc ggccgcctgc
    54901 tcgttcgccg tgccggcgag gccgatggcg acgtcgcacc gggcggccca cgctccgacg
    54961 ccgtcccggc ggacggcgac ctcgccgcca cccggcagga gggcccgcct caccggcgat
    55021 cctccttcgt cctggatgga cgcgcccgcc cggacgaggc cggcgagagc gtcgccgtcg
    55081 ttgaacggcg cgagagcgag gacgaagtcg aacgagtccc cggatcgccg ctcgaggagg
    55141 agcgcgacgt cgaggagctc ctcgacgcgc cgcccggcgt cccgccggct gcccggcagg
    55201 aggaggacga cgggccgccc gtccttgcgc ctcggctccg gcgaaggggc cctgtcgatc
    55261 agctcgacga gggggttccc ccagccgagg acgggcacgc cgcagccctg gaagcgggcg
    55321 gccgtctcgg gatcccgcgc cacgacgccg cagctccgcc tcatgagccc ggtctcgaga
    55381 ccgttccatc ggtgtcctcg cgctccgcgc tgcgacttcg cgcacgcgac gaagaggaac
    55441 ggggcgcgag agagacgaca ggcgacgagg ggcacgacgt ccccgacgcc gatcgcgagg
    55501 tccatgcggc cgcgctggcg gaccgcgaag cgcatctgcc gcgtctgcac gccgagccac
    55561 ccggcggaga ggtcacgggc gaagcggccg aggccgagcg tgctgaaccc ctcggccgga
    55621 aggtcgctcg cggggccgtc cgtcgggatc cccgcgtcgc ggtacgccgc gccggcgccg
    55681 accatcggga aggcgaggag ctccctccga ccgttcctgc ggaggcgcgc ggcgacctcc
    55741 cgtccgatca cgtcctctcc gtgtccgttg ctgaagatcg cgacccgcgc tagggttccg
    55801 gcgtgccgcc cggcctcttc cgcgtcctcg taccgacgtt cattctcttc gtcctcgtgc
    55861 gcaccggctt cgacgtcgcg gtccggggct tcgtcgatct ccgctcctcg acgcttctcg
    55921 aatacctggt cgtgcccacg gtacaaggca tcgccctctg gctctttctc ggcggcctcc
    55981 ggcgaaggat gccgctccac gggaaaggac ggcctcagaa tcgctgacct gacgacgatc
    56041 tccggccaac gaggacgact gcgacgaggc ccgacgcgat gagcgtgagg agcccggcgc
    56101 ccctcgctcc gaaggggtgt ccctcgtcgc ggagccggat gaggccccgc gtccgggcca
    56161 tccaggccgc gagcccgcct tccgtcgagg tcgccccgag gcccggcccg acgatggcgg
    56221 gcacgtaggc cccgtcgccc gtccgcttgc ccggctggcc gccgtcgttg acgacgccgt
    56281 acgcgaccca ggacgccgtt cccgagaggc gcgcgacacg gacgatcccg ttcgcggcag
    56341 cggccgcggc gaagaagccg gaaggctgag cccactggcc cgggttgagc gtaacgtcga
    56401 acggactgcc gacgggcgag ccgttcgacg agccatcgag tacctggagg cggagcgtga
    56461 tcggcccgga gccgtcgggg ccggcgtgga ggacggcgac gttcgtccgg ttcagagagt
    56521 ccgccgccag gccgtagatc ctcgcctccg tcgtcgcctc ctggcccggg tagacgcacg
    56581 gcgtgaagag gccgaactgc ccgccggaag ccgcctgcga cgccgttcgc gcgccgatga
    56641 agacctcggg caccgagacc cccgagaccg tcacgcggac ggccccggcg tagctcgcgc
    56701 tccccttcgc tccgatcgtc gcgccgtgcg agcggaggta ctgaatcgcg tcggcgagga
    56761 tcagctgccg tccggccggc acgtccaggg cgaccgtccc gcccgctccg ccgacgggcg
    56821 cgagcgactc gacgtaggag agcgagaggt gcgcggtgct ggcgcccctg ttcgcgacga
    56881 cgagctcgga acggaactcg gccgtctcca cgaggacggg cagggtgatc gtccccgagg
    56941 cggcgccggt cgcggcgacg ggcggtacgt gggagccgtc gttcgtcgag ttgtcgttga
    57001 cgacgccata ggtggaaaac gacccgcccg aggacgtccg ctccaccgtc acccaaccgt
    57061 tcgagaagcc caccccgtcc aggaccttgt cgaactggta ccagccccag gagggaagcg
    57121 tcagggcctc ccgcacgacg gtgctcgccc cggtctcccc gttgaacgcc gtcacgcgga
    57181 ccgtgaccgg ctcgtcgtcc gtcgacagga cggccaggtt ggagcgatcg gcggcgctcg
    57241 atctcagccc gtagaccgtg gccgagccgg tgagccggtc cgccgagccg aggccgacgt
    57301 aggcgagccc tgcacgaccc tcgggcgacg cgacggccgt cgtcgttcgc gcggccgcga
    57361 cgacacggcc cccctgtgac tcgacgacga ggacacctcc ctgcgccccg gtcgtcggga
    57421 tggcaagccc tttggtcctg agcgtcgaga tcatgtcggg gaaccggacc tggccgccgg
    57481 ccgggatcgt ctcggagacc gtcccggacc cgagcttctc gccgagggag gccgtgtagc
    57541 ggagcgtcac cgtcgtcgag gagtcgcccg ggttggcgag cgaaagctcg gtcgtgaagt
    57601 gtgcggtccc cgaggtcacg tccaggacga tcgggacgat cgtcttcggc ggagtcgcgg
    57661 cgtccgggtt catcgggatg gagaggatcg tcccgcttcc gtccgagaga tcgacgcccc
    57721 cgtccctgag cggtacgaag aaccggacct gcttcgtccc gagcgaaccg gggtcgggag
    57781 aaaggggtga agagacgaag agcttcacgg cgaagacgac gtgcgtcggc gcggagcggc
    57841 cggggtagta ctcgatgtcg gcgaggatcg gcgtcccgtt gttctccgag ccccggaccg
    57901 cgtcccagaa cccgccgccg tccgtctgct cgaaggtccc cgacgcgttt cccgggttcg
    57961 ggacggtgac gtcgaggcgg ttgaaggtcg ccgctcctcc cgaccgggag agagtcagct
    58021 cgtagagttc gtcggggttg ttccaggtct gatacccggt gtaggcggtc gtttcctgca
    58081 aaacgaaccg ctcaccgttc tcgaggagtt caaatgtgtg gacgtactcc ccctgaggct
    58141 gaagccctcc gaacccctgg agcacgaagc ggttgccgtc gcgccggagc gtgaaaccgg
    58201 cgtcgccgtc gtcgggcctc ccgttgccgt tcgtgtcggc gaggatgcgc gcttgccccg
    58261 agatgaggac gtccgtgagg tcggctcgag cgggaacggc gagcgagagg accaggccga
    58321 acgacgcgag ggtccttgtg gacgtcatga gagcctccag cccgggaact gtggccggag
    58381 ggagtatagg cgtcggctgc gcggggtgtc aacccgctcc gccacacccg tggcccgggt
    58441 ccttcagcga ttcgccagga gcctggagat ttcttccggc tccggaaagc ggccggtctc
    58501 tctcttcgag aagacgagcg tcccgtcgac gacgacgtcg aagacgcctc cggagccttt
    58561 cgtgagagtc gcctcgacgc cgaacgctcc cttcagctcg gccgccagac cggcggcccg
    58621 gggctggtag tttcaaacca cgcagtaggt gatctcgacc ttcatcgggt cctcccttcg
    58681 ggaggcgcat tctaggatcg accccgcccg cgcccggacc cggggaacgt ctccggggaa
    58741 gagtccgtta aggattcggg aggaggtccg tttcatgggt gatgacccga gacaggaggc
    58801 acccgtgagc gcggcgagac cggttccccg gcccgttcct ccgacacctc tcgaggcgct
    58861 cttccgggag tacggcgacc tcgtcttccg gacggcgttc cgcgtcgccg ggagcgcggc
    58921 cgacgccgag gacgtcctcc agaccgtctt cctccgcctc gcctcgccgg ggaaggccga
    58981 gcggctcggc gccgagcccc gcggctacct ccagcgcgcg gccgtgaacg gggccctcga
    59041 cctcgtgagg acccggcggt cggcggcggg aacggtcccg atcgaggagc gcgccgacgc
    59101 gttcctcgcg aaggagccgc ttcccgacgc cgcctccgag gcccgcgagc tccggggccg
    59161 cctccgccgc gcgctcggga agctcgctcc ccgccccgcg gaggtcttcg tcctccgcgc
    59221 gctcgagggg ctcccgaacc gcgaggtggc acacctgctc gggtcgtccc ccgcgaccgt
    59281 cgccgtccag ttcttccgcg cccgaaccca gctccgaaag gagctcctcg ccgaaggggg
    59341 aggcccgtca tgagagacga catcttcgag aaggccgtcg aggccgtccg tacccaggat
    59401 cccgatcccg ccgccgtcga cgccgcgctc gcgcgcgttg cgccgaccgt ccaggcggcc
    59461 gccccggccg cgcccgccgc cgccgaagag gcgccggccg tcttccggag ctgcgccgac
    59521 ctccaggtac tccttcccgc gttcctcgcg gggagcctct cgccggtgcg ggcgctcctc
    59581 gtcgaagacc acacgcgctc ttgcgtccca tgccggaggg cgctgaagaa cctccgcgag
    59641 gggaccccgt ccgcggcgcc cgccgccccg gcggcgcgcc gccggccgta cggcgcctgg
    59701 gccctcgccg cctcggtcgt cctcgcggcg gcgctcggga ccttctggct cgtctcccac
    59761 cgcgccgccg ccggaccggt tgcccgagtc gacgtcgtcg acggtctcct cttcctcccg
    59821 gccgaggggc gtcccctcgc gccgggcgcc gagctcgcgg gcggccagca ggtccggacg
    59881 ggccgctccg gtggcgccgt cctcgtcctc gccgacggat cccgcgtcga gctcgccgat
    59941 cgttcccagg tctccatcgc gcgcggtgcg ggcggcacga ccgtctccct cgcgcgcggc
    60001 cgcgtcatcg tccaggcggc gaagcagcgc tccgggcacc tcttcgtgag gacggaggac
    60061 tcgctcgtct ccgtcaccgg gacggtcttc gccgtcgcga agggagcgaa ggggacgcgc
    60121 gtctccgtcc tcgagggaga ggtccacgtc gacgacggcg cgggcgagaa ggtcctccac
    60181 ggcggtgacc aggcggcgac gcaagggatc gtcgcccggg tctccctcga ggaggagttc
    60241 gggtggagcc gcgaccgcgt cgccctcctc gagctcgcgg gtgaggtggc ccgcgtccag
    60301 aatcagctcg cggcggttcc ggtctcctgg acggggcgca cctcgacgcg tctcctcgac
    60361 gcgatgccgg acggcaccgc cctctacgcc gccctcccga acctctcggg caccgtggcc
    60421 gatttctacg cggcgctcgc tccccggctc gaggcgaacc cggtcctcgc ctcctggtgg
    60481 agcgagggcc cgcgggccgc agagcggaag gccgaggtga aggagctcct cgacacgctc
    60541 cggacctggg gctcctacct cggcgacgag gtcgccgtcg ggatcgcgaa ggacgcctcc
    60601 ggtcatcccg gcgcgccgat cgcgctgacg acggtcgtcc gccccggttt ccgcgagttc
    60661 gtcgagtccg agatcgcacg cgccggcgcg ggcgcgaagg tccggatcgt cgggagcccg
    60721 gccgaggcgg ccgaggacac gctcaacctc tggctccggg acgacctcct cgtcgcggcc
    60781 ccgaacgccg gcgcgctcgc gcgcctcgag tcggcgatcg ccgcgccggg agccttccgg
    60841 agcggggcgt tccacgcccg cctcgcggag gcctaccgcg acggcatcga tctcctcgtc
    60901 ggcgtcgacg cgaaggcgct cctcgcccgg gccgcgtccg gggcgaaccc ggagagcggc
    60961 gctttcctcc ggatgagcgg tctcgccgac gtcgagcacc tgatcgtgga gcggcgctcc
    61021 gcctcgggcg tctcgaccgg ccgcgcggcg ctcaccttcg gcgcggagcg ccgcgggatg
    61081 gcggcgtgga ttgcgcctcc cgccccgatg ggtgccctct ccttcgtctc ctccagcgcg
    61141 acgggcgcga tcgccgtcgt gacgaaggag ccggccctcc tcgtcgacga cctcttcgcg
    61201 atgctgacgg caggccagcc cgacttcccg gtgaagctcg cggaggcgga gacgaagctc
    61261 ggcttccggc tgcgggacga cctcgccgcc gcgatcggcg gggacgccgc cttcgcggtc
    61321 gacgggccgc tcctcccgac ccccgcgctg aagctcgtcg tcgaggtcta cgaccccgcg
    61381 cgcctccagg cgacgatcgt caagctcgtc tcgctcgccg acgccgaggc gcggaagacg
    61441 gggaagccgg gcgtctcgct cgcgacggag agcgtctcgg gcctcacgat ccactccctc
    61501 gtgaccggca cggctccctc tcgtctacag tacgcgttcg tggacggcta cctcgtcgtc
    61561 gccccggaga aggcgctcct cgtccgcgcc gccgaggcgc acgcgacggg tgacacgctc
    61621 ctgacggcgc cgaagctcgt ctcgctcctc ccgaaggacg gcccgctcga cttctcgatg
    61681 ctcgccttcc aggacgtcgg cggcgcgctc ggcgcgctcg cgaacgccgt cggggccgcg
    61741 cccgggtcgg ccgcctccgg gctctcgggc tccggcgcct cgctcgcctg ggcctgggcc
    61801 gagccgacgc gcgtcgtctt cggctcctcg ggcgccggcc tgaccgagct cggctccctc
    61861 gtcgcggccg gggctgccct ggacgccccc gcgagcggag gccggtgacc gcgttcctcg
    61921 agctcgacgg cctttccgtg cggctcggcc ggcgggacgt gctccgggat ctccggggca
    61981 cgctcgccgg ccgggccatc ggcctcctcg ggccgaacgg cgcggggaag acgacgctcc
    62041 tccacacgct cctcggcttc ttccccccgt cgtccgggac ggcccgcctc gcgggcctcg
    62101 acgtcgtgaa ggagagccgc gaggtgcgcc gggccgtcgg ctacatgccc gagaacgacg
    62161 ccttcgtcgc cgggatctcg ggcgtccgct atgtcgcgat gttcgcggag ctctcgggcc
    62221 tcccgcgcgc ggaggcgctc gagcgggccc acgaggcgct cttctcggtc ggcctcggcg
    62281 aagtccgcta ccggaacctc gagacctact cgctcgggat gaagcagctc gccaagctcg
    62341 cccaggcgat cgcgcacggc ccgcgcgtcc tcttcctcga cgagccgacg aacggcctcg
    62401 acccgccggc gcggcagcgg atgatccggc tcgtcaagga gatccgcgag gcgggcgaga
    62461 tccgcgtcgt cctctcctcc cacctcctcc gggacgtcga ggaatgctgc gacgaggtcc
    62521 tcatcctgaa ggacgggaag atcgcggcgc tctgcgacct cgaggccgag cgacgtgcga
    62581 accgacgcct cgtcgagctc gaggccgcgg gcgggaacgg cgacttcgcc gaggcgctcg
    62641 cgtcgctcgg ctgcaccgtg gcgcccggaa tccgccggag gctgaaggtc gtcctccccg
    62701 aaggcgtcgg gccccgcgag ctcttccgac tcgccgggga gaagggggtc cagctccgcc
    62761 acctcgccta ccagcgcgac tcgctcgagg agatcttcct ccgcgccatg gagaacggcg
    62821 gatgacggtc cgagcgcgcc gcttccgccc gttcgagggg actccgacct cgacgctcac
    62881 ccggctcctc gtcctgccgc gctacgcctg gaaggaggcg ttccaggcga agctcgtcac
    62941 gggcgccttc accgcctcgt tcctcgcgcc ggtcggcgcc ctcgtcctcg tctggctgaa
    63001 gcacaacatc gctgcgctcg cgaacacgcc gctcgcggga gggatccccc tcccgatcga
    63061 cgagaagttc ttcggctggc tcctcgtgac gcagtcgtac ttcgccttcg cgctgacgct
    63121 cttcgtcggg ccgtcgctcg tctcgcccga cctcgtgaac ggggcgctcc cgctctactt
    63181 ctcgcgcccc ctctcgcggt ggcagtacgt cctcggcaag ctcctcgtcc tcgcggccct
    63241 cctctcggcg atcacctggg tgccgctcct cgtcgtcttc gtcctccagg cctcgctcgc
    63301 cgaaggcgcc tggatcgccg agcacgccgg aatcgccggg tcgatcctcc tcggctcgct
    63361 gctcctcgtc ctcttcctct cgctcgcctc gatggccgtc tcggcctggg tgcggaaccg
    63421 cgccgtggcg cgcggcctcc tcatcggcct cggcatcgtc ccggccgtct tcggccaggc
    63481 gctgaatggg atcctggacg tcgagtgggg cggcgtcctg aacctcggcc tcgcgtgggg
    63541 ccgcgtcctc gaggggctct tcggcatccc ggtgagggcc gggatcccgc tcggcggcgc
    63601 gtgggcgagc ctcctgacgg cgaccgctct ctccctcctc ctcctccgga ggaagctccg
    63661 cgccttcgag gtggtccggt gaccggcgcc gagctggcct ggggggcgcc gtccgccgcc
    63721 ccgaggaccc cccagcccgg tgacgaggcc gccccggtca gctcctcctc gtattcggcg
    63781 gccgacctcg tcctcttcga ggaggtctcg aagttctacg gcgaggtcct cggcgtcgac
    63841 cgggtgacgc tcgcgatccc gcccggcatc accggcctcg tcggcccgaa cgggtcgggc
    63901 aagacgaccc tcatgaacct cgcgacgggc ctcatccggc cgacgcgcgg ccgcgtgagc
    63961 gtcctcggcg tcccgccgga cgaccccgag cgtctcttct cggtctgcgg atactgcacc
    64021 tgcttcgaag cgttcccggc aggggcgacc gggtggagct tcctcgaagg ggtcctctcc
    64081 ctccgcggcg aaggacggtc cgcagcgcgc gcgctcgccg ggaaggcgct cgagcgcgtc
    64141 ggcctcgtcg acgcggcggg gcggaaggtg gccggctaca gcaaggggat gaagcagcgt
    64201 ctccgcctcg ccgccgccat cgcgcccgac ccgcgcgtcc tcctcctcga cgagccgctg
    64261 aacggcctcg acccgatggc ccgggccgag gtcctcgagc tcttccggag cctcgccgcc
    64321 gaggggaagc acgtcgtcgt ctcgagccac atccttcacg aggtcgacct cctcgccgac
    64381 cgcgtcgtcc tcgtgaagtc gggctacgtc gtggcggaag gggagtcgca cggcctccgc
    64441 gaggaggtcg tcgagcggcc gatgcaggtc ctcgtccggt gcgaccgccc cggcgccctc
    64501 gcttcgcgcc tcttcgccga gggtctcgcg accgaggtcg agctccacga agacgaccgc
    64561 ggcctcctcg cccggacgcg gacgcccgaa cgcttcttcc tcgccctgaa cgacgtcgtt
    64621 ctcacggacg gtctcgagat cgagacggtg acgccggccg acgacgacgt gaaggcggtc
    64681 taccgctacc tgatcgaggg aggggagggg gcgtgagcgg gagccggccg gagatcgcca
    64741 cgcgtgcatc ccacgaagcc gccggggcgg ccggcgaagc ccgtcttcgg cgcgacgccc
    64801 gcctccgcca gctcggcgcc gtcgtccgcc tcgagctgag gcgcctcttc cgcggccggg
    64861 aggggaaggc gacgctcttc ttcgccctgg cgcccgtcct gctcgccgtc gtcggcctct
    64921 tcgcgcgcca cgacccgctt ccggcggcgc gcgccgagcg ctcgttcggg ttcatcttcc
    64981 agaacctcgt cctccagggg acgctctact tcggctgcct cttcacgttc ggcggcctcg
    65041 tccggggcga gcagatcggc aagacgcttc accacctcct cctcgcgccc gtgaggcggg
    65101 aggtcctcct cctcgggaag tacctcgccg cgctcgcggc gaccgcgacg ctcttcggcg
    65161 tcggctccgc ggtcacgtac gtcgccctcc tctcggacgg gggacgcccc gcgctcgccg
    65221 cgcatctcgg ctccggcggg gcggagcgtc tcgtcgccta cgtcctcgtg gcggccctcg
    65281 cctgcgcggc ctacggcgcc ctcttcctcg cgttcgggca gtgggtgaag aacccggcct
    65341 tcccggcgct cgccttctgg ggatgggagc acctgaactt cctcctcccc gaagggctca
    65401 agcggctcgg cctgatccac tacctcctct cgctgactcc ggtccgggtc cccgagagct
    65461 tcctcgcgat cctcggcgag ccgctccccg tctgggtctc aatccccgtc ccgctcctct
    65521 tcaccgcggc gctcctcgcg ttcgcggcct ggaaggtgcg gcggaccgag gtcgactacg
    65581 ggatcgagta ggggggtacg gggtcgaccg gcgattctgc cgatgacgcg gcgaccgcgg
    65641 acgacggtcg ccccacggtg tcgcggaatc cgtctgggtc cgccagcctc gcgcggagca
    65701 tcgccatcgc gaggtctttc ggcacgcact ccgcgaactt gagcccgtcg agagcctccg
    65761 cgacgaggga tgccggggcg ggatcgacag acgtcggccg cagcttctcg agcgcgagag
    65821 aaacggcggc ggtgctcccc tcgcgcagcg tgatcccgaa gttgtcccgt gaggtgacgc
    65881 tcaggccctg ttcggcgagg cgctcggtga gggtggcatt cgcgcggagg ccggcaaacg
    65941 tccaccaccg gaccttctca ggcgcctcct gcacgagcgt cgtcacttcc gacgcgaggg
    66001 cggggaagaa gtcgacggcg ctcctcagtg cttccgtgcc gcgtctcgtg aggccgggga
    66061 ccgaggacgg atcgacgagg acgtcgcgaa ccgcctggca gacggcgaag gagaggggag
    66121 cgcccgagcc gagccagagg gaacgtcctg cctcctgtgc gggctccacg tacgcgatcc
    66181 gctccttcca gtcgagcccg gtgacacgcc agggccgtcc gccgaggagg agcacggccg
    66241 cccgctcgcg ggcgagctgg aacgtcgccg ggtcgacccg cccgacgtcc tccgacccgt
    66301 atctcacgga gaacagcgga tccgacgaga agacggacag gagctcgagg aacctccgtc
    66361 cgccgaagct cgcctcgctc tcggggccga acgacaggac tccctcgtcg tcgaacagga
    66421 accccgtctc cacgagatgc ccctcgaccc gctcccccgc cgagcagact ggagatcgtg
    66481 gggtcaggtc cagaatcgtg tagttcggat ccgccagcgc ccggaatccg ggcatcccct
    66541 cgacccactc gcgccatccc ccgcgcgcca gtcccccgtt ttggagcgtc agggcgagga
    66601 tctgctgggc gaggatgtgg accggcgacg gcggagggac gaccggttcg acgaagcctc
    66661 tcgcccacag cttcacgagg ccgcaagcga gcagatgcga gtcgcggctc gtggtcagga
    66721 agaggtagtt ccggcgcgtc ccgttgcgcc tcccggtgcg ccccatccgc tggaggaagg
    66781 aggagacggt cccgggcgcg tcgacctgga tcacgcggtc gaggtcgccg atgtcgattc
    66841 ccagctcgag ggtgctcgtg gcgacgatga ccgagcttcc cccttcggcg aacctcgcct
    66901 cggcctggcg cctctcgacc gccgagagcg agctgtgcga ggcccaggcc tcgacgccgg
    66961 cctcgcggag ggcgtaggtc aggtcctcga cccgggagcg gctgtcgcag aagacgagcc
    67021 gcttctcgtc ggggtggagc ctcgagatga cgtgggccgc gttctcgatg ctgcccacgt
    67081 ggtcgactcg gacgtcggcg tcggcggccg ggccctgggg cgccgcgacg acccggcgcg
    67141 atccgccgct cgagcccgcc agccactcga ggagagcttc ggggttcccg atcgtggccg
    67201 ataacccgac ccgctgcacg tcccgccccg cgaagcgcgc cacccgctcg aggaccgaca
    67261 ggaggtgcca cccccgatcg tctcccgcaa aggcgtgcag ctcgtcgatg acgacggctc
    67321 ggagattccc gaagagccac ggccggtcga ccctgcgcga cgtgagcatc acctcgatcg
    67381 actcgggagt cgtgaggagg acgtccggcg ggtcggcgag gatccgccgc cggacggaat
    67441 cggcgacgtc cccgtgccag agcccggctc tcctcccgat gaaccccgcg taacgctcga
    67501 gccggggatg gaggttgttc agaagggcgc ggagcgggca gacgtagagg acggagagcc
    67561 ccctccactc ctccgtcagc attcgcgaaa gcagcggaag gaacgccgcc tcggtcttcc
    67621 cctgcgccgt cggtgcgagg aggagcgcgt ggtcgcccgc cagaacgggc tcgatggaga
    67681 ggctctgcaa cggccggagg ctcgcccagc cgagggagtt gacgacgtgg tgctggacgg
    67741 ccgggtgcag ccgctcgaac ggctccgtca cgcggggagc tcgatctcgt ccgggtcgcg
    67801 ggcggcgcgg gcgctccgct cctggcgggt cagctcggcc tcggcgagcg tcagccggta
    67861 gtccctgcgc gggtcgaagt cgtggaactg gtcgacccgg tcgaggacct cggcgacgag
    67921 cttcttcagg aagatccgcg ggacgacgcc aaccctccct ccgagctcgc ccgtgacggc
    67981 ggcggcgagc gacgcgaggt aaccgtcgtc ggccagcgct ctcacgcgcg cctcgtcggc
    68041 cgctccgtcc gcgtacagat cgcgcacctt ccgcccgacc tcctcgagga gagggcggga
    68101 gaagccctgc agccggatct gcacggcgcg ggggttgtcg aacctcgcct ctcccgagaa
    68161 gtcgacgtgg aggcgctggg cgagcggcgc gagccggtgg gcccccatcg gcccgtcgta
    68221 gaaggcgggc gttcccgtca cggcgaggta gagacccgga tagcgcccgc cgtccacctc
    68281 gtcgacgagc tgccggaggg cgttgagcgc cttgtcgcgc acgtcgccgc gcatccgctg
    68341 gagcgtctcc acctcgtcga gtacgaagac gagccccgag aagcccgaat cgcgcagtac
    68401 gacgaggagg ccctgcagga accccatcgc cgtgaagtgg tcgacctcgc ccttcacgaa
    68461 agcgtagcgc ttcaccttcg cggcgacgtt cggctggccc gcgagccaag cgacgagccc
    68521 ctcggccatc tccgtctcgc cgtcggcgag ggcccggcga taggcccgaa gcgccgccgc
    68581 gaacgccgga gcagaacggc cgatggcgga gagccgcttc tccatgagcc ccgccgtcgc
    68641 ctcgagaagc ccctcgtggt cgcccggctt cagcaaacct tcggcgagca cgtcctgctc
    68701 cagcgcgaag aaccacccgt cgatcaccga acggaacgcg gcttcgggaa cgtcgtcggt
    68761 ggcgaggcgc tcgacgagcc gccggtagac cgtgccgagc tggtgaagcg gggtctccgt
    68821 ttccgagacc tgcacctcgc tcgtcgcgaa gccgagcttt cgagccctct cctgcagcca
    68881 gcggccgaag aacgtcttgc cgctcccgta ctcgccccgc acggccttga acccgctccc
    68941 tccggcgcgg acccgcgaga gctcctcctc gaggacgctc tcgaagcggc cgagaccgac
    69001 ggcgaggagc tccaggccgt tacgcgggac cgtcccccgc cggagagcgt cgaggaggtc
    69061 ggcgcgtcgc tgcgggctga tcacgtgtcg ttctccattt cgtcagcgaa cgtcgaactg
    69121 ggcctcgagg aggccacggt tcagtgtgac ggtgccggag gcgtcgtcca ccgcgaggac
    69181 cgggtatccg tcgacgttca ggagccgtcg gacggctggc aggagcgctg cgaaccggaa
    69241 cgcgggaagg tccatcttcc gggcgagggc cgtcttcgtc gcctggccgc cgaagctctc
    69301 caacgcctcg aggaacgccc ggacgttctc gtctgcgccc tggagccgtc cgtgcaggct
    69361 cttctggtcc gcgtacgccg ccgacgcgag gagccgcgcg atcgagtcgt cctcggcgac
    69421 cggcagctcg agctgctccg tcgcggctcc cttcggtgcg gccgccctct tcggcaccgc
    69481 gggcgccggg gcagaggcgg ccgcctccgg ctcccaccag gtcggcaggt cggggccgag
    69541 gtgctcccaa cccgcgggtc gctcttcggt cgcgacccag acggccagcg gaacgaccat
    69601 ctcctgcggc gtcgccccgc cgtggtaccc gttcttcttc tgcccgtaac ggacccgctc
    69661 gctccacggg acgacgacct tcccgccgta tgccgtcgga acgcgaggtc cctcgatgac
    69721 gacctcgtcg tcgagaggcg gagtcgtggg cgggcggtac cgttcccccg agtccggaga
    69781 cgggcgcgcg gccagctcgc gctccacgac gtgaccgtga tccgcagtaa ggatcacgac
    69841 ccgcgacgcg gcgctcgcgg cgtcgagcag ggcgcgaagc ggctggagcg tcgcgagcga
    69901 ccagcgcacg ttgagctggt cgcccttgag gaggtggtcg tccacggcgt tgaggacggc
    69961 cgcgacgaca cggttctccg aagcctccac ggcctcgagg acgcgaggcg agacccccgc
    70021 gccgccgggc cccgcgaggt cggccttgtg gaagaggagg ggaggccgct tcgcccggca
    70081 ggctgcgagc aggtccgact gtttcgagaa gccggccttc tcttcggcgc tcccgcccgt
    70141 cgcgagcgcc ccgcagagga gactcgcccg cgacacctcc gtgatcgaag gaacggccga
    70201 cagggccgac agccgtcgcc cgaccgcctc gggggccagc tccttccagc ccgtcccgac
    70261 gaggtccggt gagagcgcgc ggaagacacc gtagctcatc ccgtccagga ccacgaggag
    70321 gacgggtcgt ccctgcgcga tcggcgcgac gacgcgccgg aggacctcct cgaccggaat
    70381 ctccgaggcc gacgacccgg gcgccgagca ccacctcgcg agctcggccc cgaacgctcg
    70441 attctcgtcc tcgcgcagct cggcgagacg ggagaggagg gcgcggtacg cgctcgccag
    70501 atcgccgacg ctctcggcga tcgacagccg ctcgcgcgcc gcgtcgacgt agctgctcga
    70561 gtcgacgaac tcccggcaga ggccgaagaa ggtcgaagcc cgctgtctcc ctctctgccc
    70621 gtcgatccag cgcacgaggc ggtaggcggc gtccaccgcg tctgccctct cggggttctg
    70681 gccggcgagg agatgtctcg ccacgtccgt ccgcaaccgc tccatctcgg ccaggtcgaa
    70741 tcggccggaa gagaggccgg ccgagagggc tgcacccgcc cggcggagcc gctgctcgag
    70801 agacgacggg aggaagtcgc tcaggaaggc cccggcctgc cccttgagag ccgcaaggag
    70861 cgcgtccgca cggtccagcg cggccttcca cgttgcgaag cgaacgcctg gcgggaggtc
    70921 cttccaccgc gcggagagca gcgcctccgc ttcgtcggcc catcgcgctc cgatctcggg
    70981 cgaaagggac gtccccccga gcatcggctc gaggcgcacg gccgcggacc ggagcccgga
    71041 ggatgtgcgc acctcagcgt ggaagaggac ccgacagacg agcccgatcg tcagggcgtc
    71101 ggccgggtac gccgccgatt ccgcggcgag gagcaccgga gtcgccagtg gcccgatcgt
    71161 ctccgccagc cgccgcccca cggcggcacg cgcgttggat gaagccgacc gaaagcgggc
    71221 gacgcacccg gcgtccgaag cccactcgac gaggccccgc gcgtcgggcg cgcccgacgc
    71281 gaacccgagg cccctcgtga ggtaggcgcc ccaggccgtg tccgcgtcga ggaacccggt
    71341 cggcgagggc gggaagccct ccggaggcgc ggcttcgagc agtgcgtccg ccatccagcg
    71401 ctcgtgcagg agccggcggt ccagccctcc ggccccgaag aggtcctgga ggatctccca
    71461 cgggaccacc gagaagagct ttcccttcgc gagccgggcc aggacgtcgg cgcccagatc
    71521 ggccggggtc cggtcggtca ggaggacgag ggtctcgtcc cggcaggacg tggacagcgc
    71581 ctcccgcgcg gaaagcgcag acgggcacca cgcgacgcgc gcggggccgt ctccgacttc
    71641 gagggtctcg ggccctttcc agcgtccctc ataccggacc ccgatgctcc gcgcggccgg
    71701 gagcttcttc ctcacgctcg acacgagccg cgcgacgtgc gtcggggtcg ggagagacgt
    71761 caacgcttcc cgccctcccg ctcgatcgtc caccgcagcg tgagccgggt acccggctgc
    71821 ttcgacagct tcgtctccag ctcgccgaag aggcggcgcg cggaggcggc atcgccgacc
    71881 tcgcgcgagt cttcctcgac gacgacgcgg gagccggtcc gctcgacctc gggaggaggt
    71941 ggcgagagcg gtggctcctt cggttccggt gccagtctcg tcgaagcgag gtagtcgccg
    72001 gtcagcttca gcgcgcgctt ctccgcagtc gcgaggacgt tccgcaggcc gacggcgagc
    72061 tcgtccttcc ggagcgcctc gtccacttcg gacgagagcg cttccgcggc gaccaggaat
    72121 ctccccgaca gttgcagcgc ctggtcgagg gtttcccacc gcgtgtcccc cagagcgcgg
    72181 gcgacgtcgg ccgccgagct cagcgagcgc gcgagggcct gctcggaggc cgggaccggc
    72241 attccggcaa aggcctcgca catctgcatc tccggggcct tgcgcaccgc ctcgaggagg
    72301 tcgacggcag cccgggccgt ggcgaagcgg gacgactcgg cctccgggat cccacgcgtc
    72361 tgcagcctcc cgaggaggac gtcccggagc gtccgggccg cctctctaag ctcgccctcc
    72421 cgcttccgga gcgcctcgat caggctcgcg acgttcgtgg ccgtccggtg gctcccgccg
    72481 gcccagccga agacgacgtt cgcccgctcg gaggccctgc gccaggtgtc ctccggtggc
    72541 agctgctgcg agcgaaggac gagctcgtcg cgaatcgtgc cgacgtccac cttgaacggc
    72601 gcgccgctca ggaagaaggt ccgctgcgat tgatctgcga agaccaggat gaccaggtcc
    72661 tggatctccc tcggcaaccc cttcggcttc ggctcgtcga tccacttccg gagcatcgcc
    72721 acggtcagct cgcccggctg ctcgccgatc cgccgctcga agtggttcgc ccaggtgcgg
    72781 ccgaggacga acgccgactc gtgcatcgtg ccgagctcga gaggcatcgc caccttccgc
    72841 atcgcgtcgc gcagcggctt ctccacgtcg atccggccgt ccacggcgtc cgccgcccgt
    72901 cggacctcct ccgccacctt tcggacgagc gcgggcttca gctcctcctc gaagagcggg
    72961 tgtcccgggt agacgtggga gaggagctgg tcgatgacct tcccgagccc ctcacccagg
    73021 ttcgcggcga cggggagacg gggcgagaac gacgggtcga gcgactcgat cgcccggtcc
    73081 agctcgtgcg tgg
//
LOCUS       DAOONL010000006        72801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_208_length_72801_cov_19.039329, whole genome shotgun sequence.
ACCESSION   DAOONL010000006 DAOONL010000000
VERSION     DAOONL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 72801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72801
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208_length_72801_cov_19.039329"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLP50_01870"
     CDS             complement(<1..1093)
                     /locus_tag="PLP50_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50324.1"
                     /translation="MPRRRPRPEVDRSAPLASSPRSFDSGRAAAAIAAIAIALITVWV
                     FLPVTGFGYVNYDDPNYLRDNAIVLDGLTAEGVSWAFGTFHTGNWHPLTWLSHMADVE
                     LLGPEPGRHHATGLALHVVSSLLLLFLVRAWTGSAGVGAAVAALFAWHPAHVESVAWL
                     SERKDVLSALFLWLALGAYTAYARRSPGAPALPRLALVALGLALALMAKPMAVTAPFL
                     MLLLDVWPLGRLTLDERPTWARLRPLLFEKLPLFALVALSSVVTFVAQSRGGGVSSLE
                     VLPLTERLGHAAVSVVTYLRMLVWPAGLAVFYPHRGAPASLELAGAVLLLAALTFAAW
                     RARRSAPFLLVGWLWFVGTLVPVIGLVQVG"
     gene            complement(1128..2063)
                     /locus_tag="PLP50_01875"
     CDS             complement(1128..2063)
                     /locus_tag="PLP50_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPA50325.1"
                     /translation="MSSRARAALFFALAAACLLPWVGPAVALAGGILFSLLVGNPAPS
                     RTASWSKVLLQASVVGLGFGLSLGAVARVGAASLLYTVVGIALTLALGVLLGRLFGTT
                     GPVSTLISFGTAICGGSAIAAMAPVVKAKSDEIAVSLATVFTLNATALLLFPAVGHLV
                     GLDPARFGLWAALAIHDTSSVVGAASAYGPEALALATTVKLTRALWIVPCVLGAAFLA
                     RSGERPKFPLFIAGFVAAAALRTAIPQLAPLWTGLAAVARQLLVLTLFFIGTGLTREV
                     LLRVGLRPLAHGLLLWIVVGSATLAAILSGLIPPL"
     gene            complement(2253..3002)
                     /locus_tag="PLP50_01880"
     CDS             complement(2253..3002)
                     /locus_tag="PLP50_01880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA50326.1"
                     /translation="MDIPRIFSITESAHRIHNPFTSDKLAALGAALRLETGTRVLDLG
                     SGSGEMLCTWARDYGVLGTGVDLSRLYTEQARLRAEELGVADQVRFLHGDAAGYVSDE
                     KVDVAACVGATWIGGGVAGTVELLARSLRPGGILLIGEPYWRQLPPTEDVAKGCLAGS
                     ISDFLLLPELLASFGRLGYDVVEMVLANQDGWDRYEAAKWLTMRRWLDANPGDELAKE
                     VRARLTAEPERYATYTREFLGWGVFALMTRG"
     gene            complement(3253..3681)
                     /locus_tag="PLP50_01885"
     CDS             complement(3253..3681)
                     /locus_tag="PLP50_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50327.1"
                     /translation="MKRALIGAVLLLVLSAPGTASAGDPAGYALLDRFVVQFEEMARQ
                     GSGGEKLEAALGEMMAMARKAQGESRVDAPFFDRYTRLLRVLKLVTMKDPEGILRPVA
                     EREIGDLTRDVLGERKTDIGSLASAISRELDALRKRLDGK"
     gene            complement(3717..4301)
                     /locus_tag="PLP50_01890"
     CDS             complement(3717..4301)
                     /locus_tag="PLP50_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50328.1"
                     /translation="MNGPSKDEGFEEPGALVDAEKKAALAAFDGEAFARRVRRAVAAE
                     PARTERVWLRPVLAGALATLLLAASLAAWRHVRNAPEPDPAIVAAALRGCTFFAASEE
                     ASPSRPRIPEADGARAADLVWVVQAVVHGARRSGASGRGGPDDLAGTVLAAFAGGRPG
                     GPVEWKAVPSEDLARRLGELSRGGGLARAIAGAS"
     gene            complement(4298..4837)
                     /locus_tag="PLP50_01895"
     CDS             complement(4298..4837)
                     /locus_tag="PLP50_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA50329.1"
                     /translation="MSDMEAARTGTRCSEEDVEALLEHRATVFLVCLGFVRNRHDAEE
                     LAQETYLRAWRSRGRLREEGSVKAWLCRIARNACLDHVRRTRKGLFRPLLATTDPPDE
                     TDLDAELEDKGRRIALRKAILDLSRPLRDVLVMREYGELSYEQIAGALGVAPGTVMSR
                     LHRARARVAEAVRKHGGRP"
     gene            complement(4957..5118)
                     /locus_tag="PLP50_01900"
     CDS             complement(4957..5118)
                     /locus_tag="PLP50_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50330.1"
                     /translation="MADGEAAALAALTRDFGNEPEEAAALYRRIIEADPKTEGAHRAR
                     AAIVERARP"
     gene            complement(5082..5438)
                     /locus_tag="PLP50_01905"
     CDS             complement(5082..5438)
                     /locus_tag="PLP50_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50331.1"
                     /translation="MRIAARLALVAFAPLVVALPAGAQPRSRHDVTTYGVVWKVPGMQ
                     DALLRPGLRFGDGERTMDVTLPPGAKGKPVPAVVFANGTGLPFPTWEICRDRACSANA
                     ALTLPWLTGKPRPSLR"
     gene            complement(5562..7217)
                     /locus_tag="PLP50_01910"
     CDS             complement(5562..7217)
                     /locus_tag="PLP50_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HPA50332.1"
                     /translation="MLLPGAPAALVLALVAGLGLALGSVKVRGVGLTTAGVLFVGLLL
                     GHFGVALEPEVLEFARDFGLILFVTSIGLQVGPGFVSSLRRSGMTLNLLATAVVLLGV
                     GLAAAAHLLLDVELPAVVGILSGATTNTPSLAAAQQALREVATDPASLARGATIPPLG
                     YAVAYPFGVLGIILAILALKVAFRVSMPLEERLLERSRRSEAPPLHRANLEVTNANLD
                     GLLLEKLPLVDRAGLVISRVYHGGELLVPTHETRLAVGDVLLAVGAKKDLDDLRLLVG
                     KESDLDLRSLPTPITSKRLIVTRKEVVGKTLGELNFAARFGVRITRVSRSELEMAAFP
                     GFELRTGDAILAVGGPEEIRKVAAELGDSPRDLNYPHLVPVFLTIALGVAVGLFPIHL
                     QGMPAPVKLGLAGGPLLVAILLSRLGRVGPLVFWLPISANFMLREIGIALFLAAVGLR
                     SGAGFVDTLVKGPGLTWMAIGAGITIVPMLVVGAVGRLVFRVNFLTLTGVMAGSMTDP
                     PALAFANGLAGSDAPTLGYVAVYPLTMILRIVSAEVLVLLLAG"
     gene            complement(7680..8252)
                     /locus_tag="PLP50_01915"
     CDS             complement(7680..8252)
                     /locus_tag="PLP50_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HPA50333.1"
                     /translation="MSLLLLLLLGATPTAPPSTGERLVAAARAQVGVTTSYDGAYRRL
                     AYPAGDVPAQTGVCTDVVVRAYRHLGIDLQVLVHQDMKAAWSAYPKLWRLSRPDPSID
                     HRRVPNLVTFFRRHGATLPVSRRAAGFSPGDIVVWRLASGVPHIGFVSDRRTPQGTPL
                     VLHNIGSGAREEDVLFSYVITGHFRYPPSA"
     gene            complement(8382..8822)
                     /locus_tag="PLP50_01920"
     CDS             complement(8382..8822)
                     /locus_tag="PLP50_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPA50334.1"
                     /translation="MTTHQETAVDRFANGYNCAQAVLVAFAPDLGIDADHALRLACGF
                     GGGIARQQEICGALSGGVIALGLKHGRGENQDKARTADTYVKVRELVAAFEDAHGTHV
                     CRELLDGCDLSTPEGQERFHASGLPVRVCQPCVRTVVSALERLL"
     gene            complement(8880..9626)
                     /locus_tag="PLP50_01925"
     CDS             complement(8880..9626)
                     /locus_tag="PLP50_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA50335.1"
                     /translation="MTDPYGQLAPLYDRMAADPAIRGLYRELRRALTDAAAARGLRPG
                     VIVDLACGTGNTAIPWARARARTVIGVDVSEAMLRVARKKSRRVRWVRQDLTSLSLDV
                     SADVATCHFDALNHVLDAKDLQRVFSNTARLLRPGGLFLFDLNTRFFLEWLSTSEKLC
                     RAGPHVFTAFNAFDRKTGVATFRQLWFVRQAKLYRRVLVAVKERSYEDSEIRQLLRAA
                     GLRLEEVRVMRRLKGRPIRKLYVAVRIAPG"
     gene            9968..13378
                     /locus_tag="PLP50_01930"
     CDS             9968..13378
                     /locus_tag="PLP50_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPA50336.1"
                     /translation="MRLAPLAALALLGLGLTPAAVAEGPFRPIDSPAEAAGLPQTTIR
                     STAVRPFAFDARLFAVVMRTVASEAPSEVGTPSTLLELPHPDGGLEQFRVIESPVMAP
                     ELATKFPEIRTYRGVGVDDPTASVRFEVSPRGFSAMVLSAAGAWYVDPWTKGVVDVVG
                     SYHRRDALRGEGSPFECQAPGTPDDGEGPGTHEATTDLGPTPFPLASVGPTLRTYRLA
                     LATTGEYSVAVCGSKPTTACVAAELVVAVNRVTGIYEREVAVRMTLIANNDLLIYLDG
                     ATDPYTNSNGGTMLGQNQSNLDSVIGSANYDFGHVFSTGGGGVAYLGVICESGWKGGG
                     VTGQSNPTGDAFWVDYVAHEMGHQFGGNHTFNGSLGSCTGGNRNGPTAYEPGSGSTIM
                     AYAGICGLNDLQPHSDPYFHAKSFDEITSYVSGGGASCAVSSATSNSAPAADAGAAYT
                     IPSRTPFALTGSATDPEGDALTYGWEQLDRGTVEPAPPLVDAGNRPIFRSFNPTVSPT
                     RIFPKLADVLSGTITYGETMPTTSRTMNFRLTARDNRGGGGGVASDATTVTTVSAAGP
                     FAVTAPASAVIWAGGSTQTVSWNVAGTTASPISCANVSIALSTDGGQTFPATLLASTP
                     NDGSETVTVPDTPTTGATARVRVACVGNVFFAISRPPFTIGTPPPPPTVSTVLPSSGP
                     TAGGTTVTITGTNLGSTSAVTFGGTAATSFTVSSATQVTATTPAHAAGAVDVVVTTSG
                     GSATSTGGFTFVAPAPTIASVAPSSGSTLGGTTVTITGANLTGASAATFGGAAATSLT
                     VISAAQVRVTTPAHAAGAVDVAVTTPGGTATKSAGFTFVVPKPTIAAVAPASGSTLGG
                     TVVTITGTNLSGASAVTFGGTPAASFTVDSAAQVTATTPAHVAGEVNVAVTTPGGTAT
                     RTNGFTYVSVVPAIATVAPAEGPAAGGSSVTITGTNLTGATAVSFGGSAATSFTVDSA
                     TQIRATTPAHAAGAVDVTVTTPYGDATSTNGFTYLGAPTALRFHTVTPCRIVDTRWEP
                     DGPLAGPALQASPAERTFALVPGCGVPADALVVSANVTVTGATAAGALRVFEPGSAPT
                     EATIISFPAGRTRANNALILVSGSGEVTVRNDADGEVHLIIDVNGYFR"
     gene            complement(13454..14533)
                     /locus_tag="PLP50_01935"
     CDS             complement(13454..14533)
                     /locus_tag="PLP50_01935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA50337.1"
                     /translation="MTERDGTTTGGVERRDLLKLGAAAAAGLVAGRLSAEPAVDAATK
                     PAATGASGHPWNPRTADAMPTRNLGKTGFRVGIFSLGGQAAIEKAENDAVAVPLIERA
                     LDLGVNYVDTSARYGGEARWSERYFGQVLPKRRSEVFVATKTHDRGRDGSLKLLETSL
                     KLLRTDHVDLWQLHAMSTMEDVEKVTAKGGALEAFREAREQKLVRFLGLSGHTDPAVL
                     AEAIRRFPFDTVLLAVNAADPHHLPFASELLPLAVEKEMGIVGMKVPARGRLLAGYVP
                     PPLEEQRGRAKATRTGTLSMAEAMRYVLTLPVSTVIVGCDSIPQLEENVAIAKAFTPL
                     SGAQLAALEAKAAPVAQQSLWFRRA"
     gene            complement(14628..15986)
                     /locus_tag="PLP50_01940"
     CDS             complement(14628..15986)
                     /locus_tag="PLP50_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50338.1"
                     /translation="MDEAGAHSLASGRPRPGRVRRLRPLAPLLALLLAAAAEGAAPRP
                     AVVRESWRKVEAGPVTVVGTASDPRLVETAATLAALTALLPEIVPGAFGAPPPLVVAL
                     AHEPEHLSRISLPSRAGGRIVLAAAGGEEAREALVRAAVVALARRSRPALPEWAATGL
                     AEYLSTASVSGARATLGRLVPRHVSLLRAAGPPAGRPALFVAPSLSGDGPAAALLRAE
                     AWAVVHDLLHASPDGGERLARHAALLGEGRDAGTAFAEAFGDTERSLLLRARAHAVAG
                     RSLARFLPVASAPPGRVLPLTRTGAAGLLGGAVAKGRPAEEPAANLQEPPAPSSSAAR
                     RPSSPSLSRDVPAEVDLVNRLIDAGREEEALARLEALHASLGRDPEMQRALGWDVAEV
                     RRVVVHNRLVRRYNDAIALLNAGRLAEALPIFREVAEKAEDPGLRRLAWERATAPDGG
                     RR"
     gene            16067..16492
                     /locus_tag="PLP50_01945"
     CDS             16067..16492
                     /locus_tag="PLP50_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HPA50339.1"
                     /translation="MTTTPDHPVRPAAAALLATAATFLALDLLWLGVVARGLYERALG
                     DLLRPDAYLPAAALFYAFYVAVTAGWAVLGARSKGDAARRGAGLGFVAYATFELTSWA
                     VIRGWPALLVPIDLAWGVSLTALAALAGRAAHDALRGRR"
     gene            16489..17283
                     /locus_tag="PLP50_01950"
     CDS             16489..17283
                     /locus_tag="PLP50_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPA50340.1"
                     /translation="MSLELVVGNAALVLALMTVLWVASVRLRDASLVDPWWSIAFLLV
                     AAHTALRTGLTPGKALLLAAVAAWSLRLFLHLFLRSLGKPEDPRYAAFRAKYGPERYG
                     WFSFFQVFLLQGALVVVVSAPLQVAGAAPAPDPISVTDLLGLALFLAGFGIEAVADAQ
                     LARYRRARAKGVPSAPGPVLDTGLWRFSRHPNYFGDALLWWGFWLFALDAAWGWATVF
                     APALMTFLLVKVSGVAMLDAHMAAKKPEYAEYMRRTSGFVPRPRRA"
     gene            17448..21821
                     /locus_tag="PLP50_01955"
     CDS             17448..21821
                     /locus_tag="PLP50_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPA50341.1"
                     /translation="MSGRARPRRGLRLLLLACVAVGITATLSHGHPGPGPATRAVVAS
                     GTVVYIPAAAHASGANGASWRTDLEVHNPGGATASFTIQLLRRDADNSTPASRPYTLG
                     AQRSRRFVDVLVSEFATEGAAALRIAVTSGSILVTSRTYNLIGENPWKLPKGASFGQY
                     VPGWEETAAIGYGQEGRLVHLTQQPAASLAGFRTNLGLVNATASPIAVRVDLYRADGT
                     WLGKKDGADTSLPAYGFRQLDQVFSAFGTAADGWALVKTTTPGAKLLAFATLIDNHNS
                     GDPVFIPAERTAPVAAPTPGPTATPTRTPTRTPTPTPTRTPTPGPTPGARPNLVFYVP
                     TAWPACFVVHSASGCCTSSGCCTPSPSTTGTSYIQMFLANIGSATASGPIRFRLSIDG
                     VPAGTSIWDNATGLEFGLGVILTWEYTGTIVPGIHDATITIDPDDTIAESNEADNSCT
                     TTVNWGGAPLFVGSPARDASVDALSGGAAVPLARSAPPEPPGSAALEPSTGPIYVPAA
                     AHASGVNGASWRTDLEVHNPGATAVTFDVALLRQNADNGGSPTTKSYSLGPQQSVRYA
                     DALASLFGFTGAAALRLSSSPGPILVNSRTYNLIGPNTVNLPVDASFGQFVPGLPEAE
                     AIGYGEEGRLIQLSHRDSSALTGFRTNIGFVNTTGSPIDLKVDLYTADGTFLGTVQDA
                     ATHLPPWGFAQVNAVFAPFSASLEDGYAVVRTSTPGGRFFAFATVIDNHLTGDPIFVP
                     AQRVTSGWVTAGSGTIGSAGGTVSGGGLTVTVPSGTFSQATTLTIARNPAAATPHDRY
                     RTTDVFRLDGLPDERAKAVTLTLDVTRSPGLSGKDFVAAANEVFVPTGASLGAPPMLL
                     AATRAASRVSATIPPATAPAASGLSGAAAPALAADVRPQFTIWGIAGYAELLSAQGHF
                     RVNYPVTDLVLGSAEEIAQGLEAAYTKIAALGFEWSRRTSWPVYVSIEPFPAETTARL
                     AECVPSRWGVNSYWLHLNADRLNAQADIPLMKDAAAHELFHLVQYLYDPRNRITMATR
                     PGAWYWADEATAVWFENHFNGSSAPPQVVRENHAFMTTRGLEFPPPGDDPALAGPVQD
                     HGYGAAQLMRDLAKRKGDGAVGELVRRKGNSSVLPVDAYDLQTGANAESTWRLFVDEY
                     AQKHLYGAFPTGADVWVRTAGNRYAFASASTTGTTFNWTAPDLSARFYGIQFTDKTWP
                     AGTEVSLLLTDTEKDAISLVYRLKSGQLTRLGYHLDGDVFEVENAETFANDGTSLVIL
                     VANGRASRPYTGTAPISLSVSAGARNELDFRVTGTECPGYTQLSMSFKSALDAYDLRW
                     TGNTVEVGWSGSLYPDNQSEYSGVATLSGDRKTLTSLVFTSHSVTTMPPGTTCGPSGC
                     RMESWESITWSGVPPAGDWQPSQFHFATAGAAARAYITAFSARSRWTGFTLASQNHDC
                     TYDIDEAKTGETRFDLWLTPP"
     gene            complement(21829..22266)
                     /locus_tag="PLP50_01960"
     CDS             complement(21829..22266)
                     /locus_tag="PLP50_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HPA50342.1"
                     /translation="MLVRLLYASRALAPIDEALVASILEKAHAHNAAHGITGILCTSA
                     EGNVFLQVLEGGRAAVNDLYGRILRDPRHRDVTLLDYTEISERRFATWRMGNVRLNRV
                     NLGTVLRYSETAVLDPFAMSGKAAIALLEELMSTAATVSRDGA"
     gene            complement(22278..23009)
                     /gene="folE"
                     /locus_tag="PLP50_01965"
     CDS             complement(22278..23009)
                     /gene="folE"
                     /locus_tag="PLP50_01965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HPA50343.1"
                     /translation="MSSQAGISEPASRTSGGGTPVADRIRERIRKARGRFRANDNIAA
                     WIEDGEIDLLREEVAAKMEEVLQALVIDTASDHNTRDSARRVAKMYLTEVFAGRYVQQ
                     PPVTEFPNVGRLNELMIVGPISVRSACSHHLCPIIGRVWVGVMPNEHSALIGLSKYAR
                     LVEWVMARPQIQEEAITELAELLLDKMQPDGLAVVMEADHYCMKWRGVKDADSKMTNS
                     IMRGSFLKNPDLRREFLSLLAGRMK"
     gene            complement(23032..23160)
                     /locus_tag="PLP50_01970"
     CDS             complement(23032..23160)
                     /locus_tag="PLP50_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50344.1"
                     /translation="MASGPTQARNEVLAPGSFEWPRIDFERQVPIGFHGFWPAVGW"
     gene            complement(23361..24647)
                     /locus_tag="PLP50_01975"
     CDS             complement(23361..24647)
                     /locus_tag="PLP50_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50345.1"
                     /translation="MIAFLTLFLGIVVGPQAVELSAGPGVARIELSLDGSTVASLGPP
                     WVATLDLGRELAPRELVAVAYDAAGKRVGEATQWINRARPEAEAGFALERDGAGRVSA
                     ARLFWRCPVSPDPASIAVTFDGMPLEASDPARIRIPSRSPDTAHVLIADLTFPGGIVA
                     TAVTAFGGRTKKESVRELSAVPIRILRGVRLPRRVEGMHGWFESNGRALSVVAVEEGE
                     AEVVFVQAGTIGPDFERLLVESFQAPPGARPVDLRKDSRFLFLSSNPRASVGPNAVTR
                     LFPISRDYPSRGGGFLEVGATPFANEPPTPPRIAEAVATAGLSATRRERRRAVVLLLG
                     AGARDTGDIDAPTVRRYLARLRVPLRVWRLAPAEPPAEADWPGAVDASTIERLGEAFG
                     VLRDDLKSQRVVWLEGRLQPARVEVTAKAAGLVVAR"
     gene            24987..25547
                     /locus_tag="PLP50_01980"
     CDS             24987..25547
                     /locus_tag="PLP50_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPA50346.1"
                     /translation="MPRDGSPPSGRWWARPGHSGGRWALNPDFRDLLSAFSAAEVRFL
                     VVGAHAVSFHAVPRYTKDLDLWVEPTPENAARAWDALARFGAPLEGVGVEDLSTPGIV
                     IQLGVEPNRIDILTRVAGLEFGAVWERRVTSTYGGIAVAFPSLDDLLASKRACGRPQD
                     LLDADWLEKAKKNLERSHARPPSSGE"
     gene            complement(25741..26796)
                     /locus_tag="PLP50_01985"
     CDS             complement(25741..26796)
                     /locus_tag="PLP50_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50347.1"
                     /translation="MRASSRPLAVLLVVLPFLAARPAPAEHVTLPAGARLHLEPDPGS
                     PALTMLDEETLVEVLERKDDWVRVGYGAWRGWAREAAPVSLLSERGGPNVPQEWVSGT
                     LLARARSLMREGGREVPFARRTLVTDVRDDALLARLEKAVQAATASLGDRWKLRVPVK
                     PGSYVFLFESAEAERKLAAVACGPVGPAGSRVVSLALAGDGEASTLERLLHEVGHLYA
                     RELLGDGVPNWVEEGLADAFVEGGTAPSRSAWSRRPLPVRPASSLPPPSVPELFRAGP
                     ELFERGPAGEALRAETISFFRYLSDGRLPRFRRFRAFLRRGSVGKPVDLPAFEKTLGE
                     SVAELERTWRAYRAAEG"
     gene            complement(26793..28130)
                     /locus_tag="PLP50_01990"
     CDS             complement(26793..28130)
                     /locus_tag="PLP50_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50348.1"
                     /translation="MIAFVTLFLGLFYGPAEIELSAAPGVARIELYVDGDLVSELTGP
                     WVAKLDLGPEIVPRELVAVAKDAEGRQVGEVRQWINRPRPGAEASFVVEKGSSGTGRT
                     ARLHWHSVTGSRPKSIRVSFDGRPLDVPDPSRIPLPEHSAGTTYILVADVELEDGLAA
                     TAVASLGGEKLDEALEELTAVAIRIGRGARLPKDGRLEGWFRAGERPLRVAAVEEGPS
                     EVVFVVAGEALDDLRRLMPESSRSQVAVAAADRIDLPRGTRYRLQGAIPKTVDSPTEV
                     VHLFAASEDFTPSDGTFFRVVGRLAFTKRTEEPRVAEAVAVAGLSAAARGRRRAVVLL
                     LGSGAVEEGLVEAPQTRRFLSRLGVPLHVWDVSRPSAGTAPDLPPPDVAPADARPHWP
                     EAASIADLSSLLSAFRGVEKELSSQRIVWLHGRLNPARVELTAAAGPGVSLVP"
     gene            complement(28167..29201)
                     /locus_tag="PLP50_01995"
     CDS             complement(28167..29201)
                     /locus_tag="PLP50_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50349.1"
                     /translation="MRAAARSLAALLAGVLLLSGRPAAAEHVTLPAGARLHRDPVVTS
                     PSLVLLDEEALVEVFERQDGWILVRYDTFRGWVREGAPRSLLEERGEPDAPRGWVPRN
                     LLARGRALLRNGGRELPMDGRTLLTDVSDETLLKWLVESAVATRVAFEKFWGKSEPAP
                     PGSIVFLFESAEAERKFSSVACGVAGPPGTRVVSLALSGEGREGERARLLHQIGHLYV
                     RDLLGEKVPAWIEEGLAHRFVQASRSAESRPAYHPKRAPAPKPAPTIPEILAAGPEIF
                     LREPAAEAMRREARWFFEYLERDGKTFLEFKGFVISRGPFDAPALEKAIHRSVRSMEK
                     GFLVARELLP"
     gene            complement(29198..30535)
                     /locus_tag="PLP50_02000"
     CDS             complement(29198..30535)
                     /locus_tag="PLP50_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50350.1"
                     /translation="MIAFVTLFLGLFYGPAEIELSAAPGVARIELYVDGDLVSELTGP
                     WVAKLDLGPEIVPRELVAVAKDAEGQRVGEVRQWVNRPRPGAEASFVVEKDPGGAGRT
                     ARLLWRSVTRSEAKSIRVTFDGLPLDVPDPSRIPLPAHSAGTTHILVADVDFEDGLSA
                     TAVASLGGEKLDEALEELTAVPIRLARGGKLPKDGRLEGWFRAGGEPLKVAAVEEGPS
                     NVVFVVAGRALDDLHKLIEEVTRERSGLAVRAQANLPVGARYRFVETIPRVVRGPEEV
                     ASRFFSSEDYSAGDDYFFSAIRKVAPGDRPGEPRVADAVAISGLSAAEKSRRRAVVLL
                     LGSGATDAGPITAERARRCLSKLGVPLHVWDVSRPPAKSAKKTAPRPPGPPNARSHWP
                     EAVRVSGLTSLLGVFRSLEKDLSSQRIVWLKGRLNPAKVELTASAEKGVSLVP"
     gene            complement(30572..31618)
                     /locus_tag="PLP50_02005"
     CDS             complement(30572..31618)
                     /locus_tag="PLP50_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50351.1"
                     /translation="MRAASRSHSALLAALLLLAGRPAAAEHVTLPAGARLHLEPDVTS
                     PSLALLDAEALVEVFERKDGWLLVRYETFRGWVREGAPRSLLEERGDSHLPRELVSQE
                     LVARGRALLRNGGREIAMDGRTLLTDVSDEVLLKWLLEAVVAARVEFEYSWGMGGPAP
                     PGSIVILFESPEAEREFSSVACGVAGPPGVRVASLALSGEGRAGDRARLLHQIGHLWA
                     RERLGEGVPAWIEEGLAHTLAAAGRGAEDRKGWRSLYHPKRAPAPKPAPTIPEILAAG
                     PEIFLREPAAEAMRREARWFFEYLERDGKTFLEFKGFVISRGPFDAPALEKAIHRSVR
                     SMEKGFLVARELLP"
     gene            complement(31615..32952)
                     /locus_tag="PLP50_02010"
     CDS             complement(31615..32952)
                     /locus_tag="PLP50_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50352.1"
                     /translation="MIAFVTLFLGLFYGPAEIELSAAPGVARIELYVDGDLVSELTGP
                     WVAKLDLGPEIVPRELVAVAKDAEGQRVGEVRQWVNRPRPGAEASFVVEKDPGGAGRT
                     ARLLWRSVTRSEAKSIRVTFDGLPLDVPDPSRIPLPAHSAGTTHILVADVDFEDGLSA
                     TAVASLGGEKLDEALEELTAVPIRLGRGGKLPKDGRLEGWFRAGGKPLRVAAVEEGPS
                     DVVFVVAGEALDDLRRHLDESPRGGGLEMVLEQSSLPADTRYRFVEAIPRVDRGPAEV
                     ASRFGASEDYSAGDDDFLSAIWRVVLENRPGEPHVADAVAIAGLSAAERSRRRVVVLL
                     LGSGAKEAGPITPERARRCLSKLGVPLHVWDVSRAPAKSAPGTAPRPPGLTDARPQWP
                     EAVRVSGLKSLLGVLRSLRRELSSQRIVWLKGRLNPAKVELTASAGKGVSLVP"
     gene            complement(33159..34322)
                     /locus_tag="PLP50_02015"
     CDS             complement(33159..34322)
                     /locus_tag="PLP50_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50353.1"
                     /translation="MPAVVVTGARQTGKSTLAEVLAPGQRRYLSLDDLDVVDLARRDP
                     EALVGGAVPVTLDEVQREPDLLLAVKRTIDRDRRPGRFLLTGSANLLLMRGVSESLAG
                     RASHLTLWPMTRREQLGLGRCGLWEELLSADDSTWLEVLSGGEPGPEDWKALARRGGF
                     PTPALEMKSAADRALWFDGYTRTYLERDLQALSAISALPDFRRLMRVASLRTGQLLNQ
                     TEVARDVAIPQPTVHRWLNLLETSYILVRIPAYAVNRTKRLVKAPRLYWGDVGLALHL
                     AGLSEPAGSHLENLVLNDLLAWRDSRAERAEILYWRTTTGEEVDFVIEADGKVLPVEI
                     KATSRPRLKDVANLRAFRAEYGETARAGMLLHTGTTTEWLAPDILAAPWWKVC"
     gene            34488..35147
                     /gene="brxE"
                     /locus_tag="PLP50_02020"
     CDS             34488..35147
                     /gene="brxE"
                     /locus_tag="PLP50_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-6 system BrxE protein"
                     /protein_id="HPA50354.1"
                     /translation="MSAAAPRRLAVETGVVARISRWVTLPPTMAGCLADSDLDQILAL
                     QLTIAWAGEGLCQPPRLAWWQTDLVDGPGGGDLFSRLTPRLRHWSALEAVRETARRHD
                     ARCRREVAEPEKVRTLFNLGFRVEEQLGTRLSILKSSEASPSQVLPLPLNLDSTFSPD
                     ALTAFLESLTPRVKHTAVLGGRELSGAVLNESLILARQLAAALIPLADHYPMPFARAS
                     A"
     gene            35144..35905
                     /locus_tag="PLP50_02025"
     CDS             35144..35905
                     /locus_tag="PLP50_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HPA50355.1"
                     /translation="MIGEERRFHTRLQKTTLAVVEARAYWAKSSSGISKDEQARRAFE
                     ERWFGARSAVRVRVLLESLRARFDTFPEAFAVLRRWRTIDPVTRVLVCHWHLQLSDPL
                     YRAFAGTFLPALRRSGSSATSRGEVLRWMAAIEPARWGPASRIQLASKLLTAAREAGL
                     IGPGQDARAILLPSVPDVALGYLLHLLRGVRTEGSITENPYAASIGLEGEDLEKRLAR
                     LPWLSYRKQSGVVDVEWAAPDLSSWAEAVLSLEAA"
     gene            35902..36540
                     /locus_tag="PLP50_02030"
     CDS             35902..36540
                     /locus_tag="PLP50_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HPA50356.1"
                     /translation="MSPSETPRSQLDEALAALERDLLAEGGPRISTMRNYNFAIVPYL
                     PAQELDLRRKVRALADRLRASGFRVLAISLQRLLLQRIRSLGPEVLPATTAREKRLWA
                     RDAGRALAHLGDVVGPLIEGPSGIAADVIREIEAFAEALPTPEDRSVVLIGRAGSLYP
                     FFRSSALLKHIDGHTRNLPVVLLYPGERHGRSSLSFMGVLAPDGDYRPRIYP"
     gene            36565..40191
                     /gene="brxC"
                     /locus_tag="PLP50_02035"
     CDS             36565..40191
                     /gene="brxC"
                     /locus_tag="PLP50_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HPA50357.1"
                     /translation="MLIRELFAADVTRDIPPVVYFHEQAPERLADEVREYIFTGGFPE
                     GDPRGTRGGSGIHEEVVRLLTGIRTELDKPGGPALPASWISGFFGSGKSSFAKLLGLA
                     LDGALLPGGEPLSQAFLSRDESPLAPELRKAWDDLLSKVSPMAVVFDIGSVARDGEQV
                     HSAAKRQIQKRLGYCPVSHLVADWEAKLEADGLWDAFVLAAEKALRRPWDVAKREQLA
                     PDHFSHALSVLDPERYPEPTTWIDSHAGDRSSAGSSVEEAVRAIAAMLDRREPGKTIF
                     FVIDEVSQYVHHDETRMLKLQTFVSDLGQRLKGRAWILATGQQKLEEEAAANPLSKLK
                     DRFPQSLRVHLATTNIRDVVHKRLLKKDPAREPGLRKLFREHRTDLKLYAYACEEITE
                     EEFVEVYPLLPGQVDLILRLTSSLRSRSSRARGDDHAIRGLLQLLGEVFRSCGLADAE
                     AGALVTLDAVFEVQQSALDVDVQTTLGRIMDHEICRRDPLALRAAKAVALLELIQDET
                     PTTADLVTRCLYQRLGEGNRTEAVTASLEALRGAGLLGYSERTGYKIQSSAGGEWQRE
                     RDDIGVPGELVSGIVAEKLRELLGDVDRGRLQGRPISWTVHISDGRHLKEKPLLSSRD
                     EATVVVDLRFLWNRDDRLSARWIPESGKDGLRDRLIWVSSEPALVGEVARDLARSRQM
                     IERHKDREASLPLAKKRLLSEERAGLNDLETKLGSALRSAFLEGAFYFRGREMRPSDL
                     GSGFAGTLQGAAEKVLPDLFPHFLSIAVAESELLQLLEPTLAGPSTKFLEQGLGILAL
                     DAGKYVPACSGPVPTRIRQYIEKESGVSGATLVASFGGVPYGWPADVVKAAVAGLLRA
                     HAIRIRPEDGPEISSIKDPGTRDLFRRDRDFRRADLFPAGESEVSPRDRVAICQLFED
                     RLGLDLDREDEAIAEAVFAHFPLRRERLGELRALLVGLPGPPPVPEPLVRLERALTEC
                     QRTRQVAGIVAAVKRHLDDLRDGLTTLELLRAELNDAAIEVLREADRVVSGEARQLSD
                     AGALEEVAADVEALREHLRRERPWREAVQLRPIVDRVRARYVDARRSHLAAQEEKAEA
                     ARGRIVARDGFSRLSPDAAHRVLQPISRALVDTTAAVLAPPLVEIGSGALQRLVRAEQ
                     EANERLDAELARLDSGAITVVPLDVRLSGREVRNGEELEALLRELRERIGAVLERGEK
                     VRLL"
     gene            40244..40969
                     /locus_tag="PLP50_02040"
     CDS             40244..40969
                     /locus_tag="PLP50_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPA50358.1"
                     /translation="MSSSRKNSEDARVVREVRVEDLLGRLNEVEERNSPGTLFAIGET
                     SLLHGTPRAAIVGARDASELGLARARKLARLLARRGVVVVSGLAEGIDTAAHWAAIRA
                     AGRTVGVLGAPLDVVFRQADVALRTLMEREHLVVSQFPAGHPVLRSNFPRRNRTMALL
                     SHATVIIEASDSSGSLSQGWEALRLGRPLFILKSVADDPRLTWPAEMTRYGATVLSES
                     GVEALFDALPPPEEPGWLDRLAF"
     gene            41406..41615
                     /locus_tag="PLP50_02045"
     CDS             41406..41615
                     /locus_tag="PLP50_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50359.1"
                     /translation="MLLVDDVVTKGTALLAAARVVSAARPGSEVSAFALVRTMGLLPD
                     VERILDPCLGRISLTGMPPEAHREP"
     gene            41612..42652
                     /locus_tag="PLP50_02050"
     CDS             41612..42652
                     /locus_tag="PLP50_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50360.1"
                     /translation="MRRRRDDPEQKRLLTREEFQALLSARDTEGLRKMLWELYVRGSE
                     DLRRRIETVALPVGERPKKVAPPPVDGEWHLGEVRQFVALARSGAYTGGTRRVSPSER
                     TRWRLPFRALIDDSGRLLTQGDVANGTEAMSALLDLALDCRDTYYFRTEDPVSALRIV
                     ASDLVDLLWRANLEAHGFPALVARAPRDLLRWESPWGWTRMGTRTIAAKERSLADVLA
                     ALLPSHDAWVAMAEAWLQVFAATHGGRAAPRSAPNRNLPSQYVPPTPSDWKRWHELLY
                     ERLHGSESHHLLERIAAVPERPGVDLWLLRVRLARDAGRAEEARCIARAALGEFPGAR
                     ELQAIAREVGLR"
     gene            42800..46801
                     /gene="pglX"
                     /locus_tag="PLP50_02055"
     CDS             42800..46801
                     /gene="pglX"
                     /locus_tag="PLP50_02055"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-6 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HPA50361.1"
                     /translation="MTLTPSVRRRLSETIRDLRARLLADLHEAVVAEYRLTLPIARSG
                     LSEAAAIKRGRLEAFLEEKVRAGRARDAAGKEAIRKRALEGAVKEAASTLLNRLVFVR
                     RLEALGLSRPLVLTGGWRSQGFRELISFAPGLTIDSGDPAEGYPFLLKLLFDELALDL
                     PGLFGEVGVSALVPVPLPTLRAVVEALDAPDLAPAWDDDTSLGWVFQYWNDPEREALD
                     AKVLTGGKIAPHEIASKTQMFTERYMVEWLLQNSLGDLWLEICRKNGWKADAEEVFPA
                     LEERRAAWRTARARDEVPPTEPMAVSEGLEERWKWWVRKPAPEEVPAGTPPSIRDVKL
                     LDPACGSGHFLLSAFDNLAALYQEEARHRGVVWSDRQIAEEILERNLFGVDIDPRAVQ
                     IAAAGLYLKARALERNARPRALNLVAPALGLASLPPGDPALAALRDELRTEAGIPHDL
                     TDAVLTALSGVDHLGTLLKVDEAVEEAIRKAAGAPKVEAVQKKLFGGDPPAQAKLDYD
                     AARASILEKIEGFLGRHRGEDDLGLRLDGEALAAGLRFLRIVKSGTYDVVVGNPPYQG
                     ASRMAEAGYLGRTYPRGKADLYAAFLERALDLARPGGLSALVTMRGWMFTAQYQALRE
                     HLLTTYDLRVLGDVDRGAFDEVPDEVVAAAMSVFRKAPPPGLPSVALQPTALTDKSRD
                     RERTERKRAALLAQVGRYEFEVARLKVVAGEPLLYWWSEEFLKEYAEAPKLGDVAPAR
                     QGLATADNARFLRRPWECRMKPGPISRDVADAEAVSFGPWAPFVKGGEGRLWLEPLSD
                     LVLWDRQGLELKTFTEFWYGSASRNIKNEDVYFRRGVAFSAIGNAFGARAHRAHSIIG
                     HMGASSFPEDMPMAVCLMNSWTSRFVLASLNPGLHFEVGDVNRLPIFPVESSAEIFET
                     LDAAFTEHEAARETSVEFKRPGPSAWTSAQAWAQEAVDRTKGSPLPPYEPVYEAASAF
                     QHVSFAFGVAMGRFGANGEGILEVAPADALPGGILFLSEATGRDSLAHSACGPLHAAW
                     ADHGAAVGEGDDLRSWLRKSFFGFHRKGYEDRPIYFPLSSARKTFVAWVSIHRFGPDT
                     LKAVLADHVQPELRTLEGELEDLKAARQASDRRERGRAEKRYAEIQKQLAELLDFVGA
                     LRQVAEKGPPPVDASTPPREADAPFEMDLDDGVMVNAAALWPLLAPQWGKPAQWWKEL
                     AKAEGKKDYDWAHLAARYFPSRVEKKCKDDPSLAVAHGSFWRLHPARAYAWELRLTDE
                     IGPDFTIDEEGSDTARERFLAEEPERAADLRKKEQDRKRRKLLRTGGSSEELFDEEAE
                     AEDAPAETEEADA"
     gene            46794..49670
                     /gene="pglZ"
                     /locus_tag="PLP50_02060"
     CDS             46794..49670
                     /gene="pglZ"
                     /locus_tag="PLP50_02060"
                     /inference="COORDINATES: protein motif:HMM:NF033443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-6 system phosphatase PglZ"
                     /protein_id="HPA50362.1"
                     /translation="MPERPVSAALEAAVREEVRLHGIVFWLDREGTYSAFVDDLVARR
                     AAGTFPVPVVAFRGSYLETLLALEPYGSGLDPEPLLVHLPGFTEETVRETPLFELYET
                     GVRFRKALDTLVREAAAGKVAPKAVEGILAGPGLSLEAADAALAQAMEAATGGPGAFL
                     SALKPEVLLEGLVTGEADLLARISDATGVAALLQALERQTGMDEAWRGFAGAPAPGAE
                     GRGAVEQAASAAVSWVLAMEYVHDLKRAPELPALRPLAKAGKPVVATCGRLAAHLRSR
                     QPELYERLADETEGVLAGELSTMRAEDLGRIDTFRSEERTILEAAIEAVRAGAHARAL
                     EWAEARRVTGSFWAQREPTHAWEWSLVREAATLGRLLAEMPVPLAGARDVADALERYT
                     REAHRVDQAHRRLEQRRVALLEPRLPHFGALQGALALLRRGYREWSDRLARDFTALCR
                     MYGFLPPSSIRQRSFWDEVAHPLASALGTTAVLLVDALRFEMAAELAEELRTERRDGA
                     GVSLSGRLAELPSLTAVGMNALAPVATDGRLRPIVTNGSFEGFRTGEATVRKPADRAK
                     AMGARSTGAPALRLDLKELVELDVSELTRKLRGARLVVVTSREIDDAGEANVGLATFE
                     QTLRQLRSAIFLLEGAGAKRIVVTADHGFLLLDETTAVRPYGSRRDPERRHVFATERR
                     QETGMVAVPLAELGYDGVDGNLLFLEDTSVFATGVTGATFVHGGNSLPERVIPVLVLR
                     EARATATGEAEWRIEAEASAGLLGFSCLKVRIAPAPGSLAFAERSEVTLALRAAGRTD
                     VDVLLRDARGNGTVRGGRLELRSGSEWTELFFSLEGPVDEKARVEVFHPDGVVRAAPV
                     SPEAWFGVTGRKPSGASPISPARAASAAAWESALEDEGARRIFVHLAQHGSITEVEAT
                     RLLGSARAFRAFSARFDALRARVPFETRVETTPEGKRYVREGER"
     gene            49675..50997
                     /gene="brxD"
                     /locus_tag="PLP50_02065"
     CDS             49675..50997
                     /gene="brxD"
                     /locus_tag="PLP50_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HPA50363.1"
                     /translation="MPSRRDVDHVFERLRSGLVPERGLEEFAVGIERPRGEIRRLLDL
                     AGRGEGVFKFLRGGYGCGKTFLARLAVLDAQAAGFATSFVVVSDNDLHFHRFDELYRR
                     VVQELGTSACPRGALADVVDRWIARVEEALVAGLEDADGAGFDEAVARRLAEEVTSRT
                     AGKAPEDLARVLRTIFALKQAGELSDAGALLSWLSGSENVAAGVKRAAGIKGDIGSRE
                     AMDYLRGVLEIVKAAGYKGLVIVIDEAETILRMRQDVRGKSLNGIRQIIDAAAQFPGL
                     FWLFTGTPELFDTRRGVAGLAPLHERIQFLRHGESASLRQPQLALTPFDETRLREVAL
                     RLRELYPASDPHRVEAKVTRTVIDALVAKVTEGFRGDVGVVPRQFLRRLVDLMDLVEE
                     DPTFEPGREGAPRTEDLNEPERRVAKGEPPFDPEPEDEEGYSPALVEV"
     gene            51007..53121
                     /locus_tag="PLP50_02070"
     CDS             51007..53121
                     /locus_tag="PLP50_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA50364.1"
                     /translation="MTVLRRFPPRLREAIGQRLGWPALRPVQEQAAEALLDGRNAVVL
                     APTAGGKTEAALFPILAGLVENEPNGVGALYVSPLRALLNNQEERFGLYTEMVGLESF
                     VWHGDRTASRKRRFLLEPAAILMTTPESLEAMLLSQRVPVRRLFADLRAVIVDEVHSF
                     AGTDRGAHLMSVLERIAPISGHDVQRVGLSATVGNPEAILRWLAGSSRREGQVVQPAG
                     GRSARELLVLHREAPGDLAREASRLGRDRKVLVFCASRALTEKVAGRLRDLGTDVFVH
                     HSSVSLEERQAAEERFRSSGPACIVATSTLELGIDVGDLDRVLQVDAPSTVSSFLQRM
                     GRTGRRDGQTSNTTFLCQEAESVLLAVALVELAREGWVEPVPPQTRCWPVLVHQALAI
                     ALQHGGIQPGEVRALPERVPDFSGISSPEIGELTEHLVAEGYLSSAGGRLLFGERAER
                     ELGRRNFLELYAVFSTPALYHVETRARQELGTLEQSFVERLEDGKSCFVLAGRPWVVV
                     SVAHKERRVEVEPAPRGRTPTWGGFLPQILSREVCARIRRLLTEEAEPEYLAPAARRA
                     LALLREDRGTVLAQSGMPLLPGSEAVRALTYAGGRVNYTLKAVLESLHLVRVVADNFE
                     IRVEGSTATREAVESTLRAIAVPGYFDDAERVERLRAGLPPFRLSKFQPLLPARFARE
                     VIGRYFLDFEGTAQFARDVVGS"
     gene            complement(53171..54259)
                     /locus_tag="PLP50_02075"
     CDS             complement(53171..54259)
                     /locus_tag="PLP50_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPA50365.1"
                     /translation="MAETAYRPYQPDFVTPPGETLRETIEAWDITQADLAERTGKTLK
                     AINEIVQGKSAITPETALQLERVLSVPAAFWLNREARYQEFKARVAADEKSSKGEDWA
                     RGFPVRAMEKRGWLPAGKNPAERANALLVFFGVASVDAWEVVWGDVAAQYRKARAFEG
                     DPKSLFAWLRRGELLARDVETKPWSAAGFTDVLRSLRSLTLRPFDCALPEAVAHCASV
                     GVVLVVEPELPKARVFGATRWLSPTRALVQLSFRYRTEDHFWFTFFHEAGHLLRHGKK
                     DFFVEDGTSTSREEEEADRFAAETLLPTRSLAGFLRSWRRDEASLRRFAEEQGIAPGI
                     VVGRLQHEGAIPPSHLNGLRRRIGATPA"
     gene            complement(54252..54590)
                     /locus_tag="PLP50_02080"
     CDS             complement(54252..54590)
                     /locus_tag="PLP50_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killer suppression protein"
                     /protein_id="HPA50366.1"
                     /translation="MELHFKSRKLQKQAESFRDLVRAHGDRRAQLIRRRLDELDAAST
                     LADVAKLPGPRCHELVGDLAGILSVDLDHPYRLLFRPLADPPPLRTDGGLDWGRVTAI
                     LILGIEDTHG"
     gene            complement(54708..56390)
                     /gene="ettA"
                     /locus_tag="PLP50_02085"
     CDS             complement(54708..56390)
                     /gene="ettA"
                     /locus_tag="PLP50_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPA50367.1"
                     /translation="MAQQYIYTMVNLRKVVPPQREILKGIYLSFFPGAKIGVLGLNGA
                     GKSSLLKIMAGVDREFNGEAFPAEGTRVGFLQQDPWLDPSKTVLQNVEAAVEPVKKLL
                     ARFDEMGEKMGESLPDAEMDKVMAEYGKVQDAIEAANGWDLDRTLETAMDALRCPPPD
                     ADVTKISGGERRRVALCRVLLEKPDLLLLDEPTNHLDAESVAWLEQHLAEYPGTVVAV
                     THDRYFLDNVAQWILELDRGAGIPWKGNYTSWLEQKKTRLEQEEKAETAKQKTLEREL
                     EWVRMAPRARHAKGKARLQAYEQLLSESGAESRGETNEIYIPAGPRLGDVVVRAEGLK
                     KAYGEKLLFDDLTFDLPKGGIVGVIGPNGAGKTTLFKMIMGLEKPDGGVLTVGETVFP
                     SYVDQNRDRPDPTKCVWEEVSGGEEKIMVGRREMSSRAWCSMFNFKGADQQKRIGDLS
                     GGEMNRLHLARTVKKAGNLLLLDEPTNDLDVDTLRALEEALLSFAGCAVVISHDRWFL
                     DRVATHMLAFEGDSKTFWYEGNYQEYEADRHKRLGAAADMPHRIKYRKLTTG"
     gene            56528..57058
                     /locus_tag="PLP50_02090"
     CDS             56528..57058
                     /locus_tag="PLP50_02090"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPA50368.1"
                     /translation="MRRALPLLALLLAAPLAGQENAIPRVPISPRGAEEIAVGNGTMR
                     VEYGRPFAKKRKVFGGLVPWGKVWRTGANAATTLRLDVDVTLGTASLPKGTYTLYTLP
                     GPKEWQLIVNRQTGQWGTEYDPKQDVVRIPMKVLSRHDHLEAMTIDFLPEDAFRGSLR
                     ISWEKKMLVVPYAFRR"
     gene            57096..59321
                     /locus_tag="PLP50_02095"
     CDS             57096..59321
                     /locus_tag="PLP50_02095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPA50369.1"
                     /translation="MTSKSPRAILVAAIAAALALPLAAAPAKRAATIADLHAVQGVGE
                     PVIAPDGRSIAFTVTTTDLPAVKRWTNLWRVDADGKNARALTVLDKRDASPDFSPDGA
                     TLAFLSTRSGAPQVWFLPTAGGEPEKKTDLPGGVGAFRFSPDGKRLFLLADVWPACGA
                     DLDCNRKKDEAMEKGKMKAVVADSLFVRRWDSWEDGKRTHILLMDLSDPKAPLVDLTP
                     GDFDSPAFSAGGGADFDVSPDGKELAFTSHRDPFPAASTNVDVYVVPVDGTEEQRKAP
                     KNLTAKNPAADGAPRYSPDGKHLAFRMQRTPGYESDRFLLALHDRAAGTSRVLTEAFD
                     SWVGDYRFSPDGKKVLFTADVRGRTPLHELDLASGKIEVVSSTGFLDTFQVAPDGTWA
                     VLARRQMHLPTELYRLTLAGKERGKVTPLTSRNEKLLSEVALAPMEEVFVDSPTGKKV
                     QVLLVKPHDFDPAKKYPVIVNVHGGPQMQWADAFRLDAQVYPGAGYVVAFPNPHGSTG
                     WGQDYTAAISGDWDGKVMKDVLAVADWLSVQKWADEERMGAMGWSWGGYAMMWLAGHD
                     HPFKALAAMMGVYDLRSMYSMTEELWFPEWDLKGTPWQNPEGYAKQSPSSYVTKFKTP
                     TLVLTGQKDFRVPYTQSLAFFTDLQKMGVPSRLVVFENGSHWPGWYDMVLYYSAHLDW
                     FHRYLGGAPSPYDPKALVRNDVFGKEKDEAKGPAKDEAKPAPRKAVPPKPEEKKVPAN
                     P"
     gene            59445..59792
                     /locus_tag="PLP50_02100"
     CDS             59445..59792
                     /locus_tag="PLP50_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPA50370.1"
                     /translation="MTIFEKGLVTILVVDALSILISIPLVLRKIGPNVVYGYRTRATL
                     SDPALWYEANALFGRRLIAACTVSALAALFLARPGLLSPDAFLPVSVVLLGAPVAVAG
                     VMTSRFVARVTKG"
     gene            59920..60834
                     /locus_tag="PLP50_02105"
     CDS             59920..60834
                     /locus_tag="PLP50_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPA50371.1"
                     /translation="MATVVANAWSARAGRSDAAGRGMARAFAVLVLPFPLVGLAIFLL
                     GRSTGAKAAAAAVAVAPLLLTLALEGAGRLSAFGKARAGGARARFREPGARALAAAIE
                     EGRLERVRFLLGSVDPDAPGTNGESLLGFALRKRQGDAALELARRGADPLRGPAGEPN
                     ALRLAAQEPLFVEVLRALLANGTSPDTVLPDDIDRPTPLVFTAMNLNAWPNVRAVVEA
                     GARLDVVDVNGRTPLARAILWRAWNEARLMVERGAPVTAGLRGPASLEEAFAATRPPE
                     AGTPERAAWDLLLAALAERGFPEPAGAR"
     gene            61050..62264
                     /locus_tag="PLP50_02110"
     CDS             61050..62264
                     /locus_tag="PLP50_02110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPA50372.1"
                     /translation="MRTALAAAALLVGAWLGLGALLRPSLTLARVRTGRVDVGPMTAT
                     LSAPGTIVPESERVISSPIDSRILRVLVQPGAAVKAGTPLVELDATGPALAAARLRKE
                     LRLREAAAERAALALEGTLASLESRVEVAGLTLSTWGASLERNRKLFAEGLVSEELLR
                     QSELDEARTRVELARLKDEMRLARRSAEVEGRSLELELATARSEAAQAERELALAAAR
                     SEAAGVVTFVATGEGAAVRKGDVLARVADLTAFRVDALVPDVHASRVAPGQEALVRID
                     DVRLPGTVRRVAPEVRDGQVAVSVSLSESAHALLRPSLRVDVEIVTGRKERAMRLPRG
                     SFAAAEGGVSVWVLRGDEALQSTVRLGLSNAERYEVLSGLAEGDEVILSEMADFAHLS
                     RVRVKGRQGGTR"
     gene            62261..62926
                     /locus_tag="PLP50_02115"
     CDS             62261..62926
                     /locus_tag="PLP50_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50373.1"
                     /translation="MIRLSNVEKVYRTDRIETIALSNVSLEVEAGEFVSIMGPSGSGK
                     STLLHVMGLLDAPTRGSVELNGRKVDSLSDGALAKVRNAELGFVFQTFHLVPDLSVLD
                     NVEVPLLYRGGGARERRRKAEAALGRVGLSARTRHFPAQLSGGQQQRVAIARAIVGSP
                     RVLLADEPTGNLDSAMGDEVLALLSELNREDGTTVVMVTHDERQAAKTHRVVRLFDGR
                     PVS"
     gene            62929..64164
                     /locus_tag="PLP50_02120"
     CDS             62929..64164
                     /locus_tag="PLP50_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50374.1"
                     /translation="MLAHTLKTAVRVLLRRKFFTAVSLFGVALTLVVLVVAAALLDHT
                     IGAYPPETRWPRSLGVFRVSLKGEGWTRSGGAGGRLLETCVRDLPGAEVTTFYSQGDR
                     EVTYRGGERLPLWVKRTDGAFWRALDFRFLEGGPFTEADDAAANAVAVVNDATRRRLF
                     GSGPAVGRTLVLDGRTFTVVGVVADVPFLRPVAFSDVWLPARTARSDDWRRAWVDDWK
                     AFVVAKSEADLPGIREELKRRVAAVDLSDEKPFDRLEAEAETVFEAIARLPLGNRPDR
                     AALLVALLGGLALAFMTLPALNLVNLALSRILERAPEVGVRRAFGATRTRLALQFVTE
                     NLVLALAGGGVGLVLSVGVLAAIDASGLIPYSDLSLSPSVFLQGLAATCVFGVLSGAL
                     PALRMARLHPVEALRGGPR"
     gene            64161..65375
                     /locus_tag="PLP50_02125"
     CDS             64161..65375
                     /locus_tag="PLP50_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50375.1"
                     /translation="MTRHLLKLVWNRRRHHLLVGLEVLASFLIVFVVAVLAVKTVGNA
                     ARPAGFVSDDVTVVEVGLRKRGGGPVVDDGADRERMRRALEALRRLEAVEAAAAAFLV
                     PYGTGRAVNSHTVNGRTLEYDYSDVTDDYLAVARLKLAAGRWFSREDDGRPYRPVVVT
                     RELAREAFGAEDPVGREIPEGGVEPGTARRRSRVVGVLEAYRPGSDYAAPAPFALFRA
                     DLANPADDVPSRIVVRLRPGTPRAFEETLVRRLGAAVPEWSLTAEPLAVAREAANRRW
                     TVPILAASLVAGFLMLLVALGLTGVLWQNVTRRTREIGLRRATGATARQVAVQVVMEV
                     WIVTALAVLSGCAVALQAPLLVPGGFVRASDAAAGAAAAAVLLFALTGLCALYPAWLA
                     TRVFPSEALRYE"
     gene            65380..66756
                     /locus_tag="PLP50_02130"
     CDS             65380..66756
                     /locus_tag="PLP50_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA50376.1"
                     /translation="MKDDAAVLVVDDDSAVAASLVLLLRQAGYRPASAATPEEALARL
                     AAAPADLVLSDMNFARGTTGEEGLAFLAALREKHPGLPVVLMTAWGSIELAVRGMRGG
                     AADFVTKPWSAERLLSTVATALALSGVGPARDSDVPSREELDAAAPAFAPLAGRDPKL
                     VRLLALAARVAPTDASVLVTGESGTGKELLAEAIHRASPRRAKPFVKVNLGGVPTTLF
                     ESEMFGHVKGAFTDARQDRAGRFAAAHGGSIFLDEVGELEPAAQVKLLRVLQDRTFEV
                     LGTSATRSVDVRVVSATNRDLGEAVARGAFREDLLYRLNLITLRLPPLRERPDDVPFL
                     AARFLAELSRRHGRPLALAPDAGEWLAARPWPGNVRQLKHLVERSVLLTGKETLGAAD
                     FAFVAGMDEGARREEPATARTLEALEREAIARCLARHGGNLSRAAEELGLSRGALYRR
                     LEKYGIAP"
     gene            66753..68060
                     /locus_tag="PLP50_02135"
     CDS             66753..68060
                     /locus_tag="PLP50_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50377.1"
                     /translation="MSWSARALLYLLVLHAALALLAVPLLRESPGWLLAAEAAFVLSA
                     LAGALLVRRLAAPMESLRDGAELLRAGELGTRLRPSGTPELDSLVALFNEMLARLQSE
                     RVRQEERQAFLEKVLEASPLGVVTLDLDGKVDVVSRAATGLLGADAGALHGRRLVEAS
                     GPLAGALSELPPGGSRVVPLGGGRRVRARRSHFLDRGFPRTFFLLDELTEELRLSEKA
                     AYEKLIRMTSHEVNNSGAAVGSLLGSLLSLAPRVPEEDRTDFVEALRVAKERTERLNS
                     FMRSLADVVRVPAPRLAPTDPNALVRSVLLLLGPLSERHGVALALEADPALRPVPLDA
                     ALFEQALFNVVKNGIEAAGRGGSVTLRTSAEGPGRIEVVDSGPGIPADVEAQLFTPFF
                     TTKPEGQGVGLTFVREVLASHGFAYSLAGPPGGPTRFDVRFGA"
     gene            complement(68188..71271)
                     /locus_tag="PLP50_02140"
     CDS             complement(68188..71271)
                     /locus_tag="PLP50_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA50378.1"
                     /translation="MKLTDLFIKRPVLAVVVSLVITIAGLQSIRSLSVRQYPRSDIAV
                     VRVTTAYVGANADLVRGFITTPLERVIASADGIDYIESSSAQGVSTITVHLRLNYDTN
                     AALTQVQAKVAQVRNDLPPEAQAPVIELETADSQFAAMYIGFSSADLDQNQITDYLTR
                     VVQPKLSAISGVQRADILGSRNFAMRIWLKPERMAALGLSASQVREALANNNYLSALG
                     RTKGSMVSVNLVANTNLQTPEEFRQLVVKREKGSIVRLGEIADVTLGAEDYESDVHFN
                     GQDATFMGIWVLPTANALEVTAGVREAIPEIQAQLPAGMKVGVPYDSTAYIQDAIDEV
                     LKTLTETLLIVILVIFLFLGSFRSVLIPVVAIPVSLVGAVFLMAIAGFTINLLTLLAI
                     VLSVGLVVDDAIVMVENVERHLQLGQTPFQAAVKAARELVGPIVAMTITLAAVYAPVG
                     LQGGLTGALFREFAFTLAGAVLVSGVVALTLSPMMGAKLLRAGDSERGFAGWVNRRFE
                     FVRKTYERQLGATLSYRPVVLTLWVLVSLLVVPFYLFSQRELAPHEDQGVVFGIIQAA
                     PNATLDQTKLFAAQVHDVYRSFPESDSIFQITSPSGGFGGMVTKPWSERERSTAELHV
                     EASQKLSKIPGVRVISLTPPALPGGGDFPVDLVIASTAEPERLTEFAGQLVGKAFGSG
                     LFMFADADLKFDQPQAEVVFDRDKVRAQGVDLADAGRDLSTMLGGNYVNRFSIQGRSY
                     KVIPQVKRAERLTPDQLEQIHVSGADGRLVPLSTFASLKTTTEPRELKRFQQLNAVRI
                     QGVTAPGVSLDEALGFLEAEAAMILPKGFTLDYAGESRQLRVEGSKFLGTLLLSAILI
                     YLVLAAQFESFRDPFIILAGSVPLALAGALLFSFLSFTSLNVYSQVGLITLVGLVAKN
                     GILIVEFANKLQETGIDKVRAVVEAASTRLRPVLMTTAATIMGHLPLVFASGPGAGAR
                     NSIGIVLVTGMFIGTAFTLLVVPSIYVLVARTRRAEETESEVDGEPEGPGLVPAS"
     gene            complement(71271..72404)
                     /locus_tag="PLP50_02145"
     CDS             complement(71271..72404)
                     /locus_tag="PLP50_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA50379.1"
                     /translation="MKKRMSLMLSLVAAFVAAVATLKYGQLRAAGAQGASFRPPPDAV
                     TTTLTREDRWPSSLAAIGTVVAVHGVSVSADLPGVVDRITFESGRPVSQGAVLAELDT
                     RQERAQLAAAEAQLELARANLERARGLRDGGVLSQADYDRALAEHTQGTARVGEIRAT
                     IARKTIRAPFSGVLGIRRANLGQYLAAGEKIVSLESIDPIHVNFSVPQQEVNRLRKGG
                     AVEVRSQDASGQETRSVARISAIDAVVDVATRNVEIQATLPNPDGRLRPGMFVEVEAD
                     LGTSTPVIALPGSAISFAPYGDSVFVVAQLKGPDGTPYRGVQQQFVKLGASRGDQVAV
                     LSGLEPGQEVVTSGVFKLRPGAAVEVDNRIRPGNDPAPMPQDS"
BASE COUNT        10333 a        26388 c        25930 g        10150 t
ORIGIN      
        1 cccccacctg gacgaggccg atcaccggga cgagcgtccc gacgaaccag agccatccca
       61 cgaggaggaa cggcgcgctc cggcgcgcgc gccacgccgc gaacgtgagg gccgcaagga
      121 gaaggaccgc cccggcgagc tccagagaag cgggcgcgcc ccggtgcggg tagaagaccg
      181 cgaggccggc cggccagacg agcatccgga ggtacgtgac gaccgatacg gccgcatgcc
      241 cgaggcgctc tgtgagcggg aggacctcga ggctcgagac gccgcccccg cgcgactgcg
      301 cgacgaaggt cacgacgctc gagagggcga cgagcgcgaa gagcgggagc ttctcgaaga
      361 ggagcggccg gagccgcgcc cacgtcggcc tctcgtcgag cgtcaggcgc ccgagcggcc
      421 agacgtcgag gaggagcatc aggaacggcg ccgtcacggc catcggcttg gccatgagcg
      481 cgagggcgag cccgagcgcc acgagagcga ggcgcgggag ggccggcgcg cccggcgacc
      541 gccgcgcgta cgcggtgtac gccccgagcg cgagccagag gaagagcgcg gagaggacgt
      601 ccttccgctc cgagagccac gcgaccgact cgacgtgcgc cgggtgccag gcgaagagcg
      661 ccgcgaccgc cgcgccgaca ccggcgcttc ccgtccacgc gcggacgagg aagaggagca
      721 gcagcgacga gacgacgtgg agggcgaggc ccgtggcgtg gtggcgcccc ggctcgggac
      781 cgaggagctc gacgtcggcc atgtgcgaga gccacgtgag cgggtgccag ttcccggtgt
      841 ggaacgtccc gaaggcccac gacacaccct ccgcggtcag gccgtcgagg acgatggcat
      901 tgtcgcggag gtagttcggg tcgtcgtagt tgacgtagcc gaagccggtc accggcagga
      961 agacccagac cgtgatcagc gcaatggcga tcgccgcgat ggccgccgcc gcacggccgg
     1021 agtcgaaaga acggggagag gacgcgagcg gagcggagcg gtcgacctcg gggcgaggcc
     1081 tgcggcgggg catggcctca ggatagcgga gaggcgggag ccccctttca gagcggcggg
     1141 atcaggcccg agaggatcgc ggcgagcgtc gccgatccga cgacgatcca gaggaggagg
     1201 ccgtgggcga gcgggcggag gccgacccgc aggaggactt cccgcgtcag gccggtcccg
     1261 atgaagaaga gggtcaggac gaggagctgc cgcgccaccg ccgcgaggcc ggtccagagc
     1321 ggcgcgagct gcgggatcgc ggtccggagg gccgccgcgg cgacgaagcc ggcgatgaag
     1381 agggggaact tcgggcgctc gcccgaccgt gcgaggaacg ccgccccgag gacgcacggc
     1441 acgatccaga gcgcccgcgt cagcttcacc gtcgtggcga gcgccagcgc ctccggcccg
     1501 taggccgagg cggcgccgac gacgctcgac gtgtcgtgga tcgcgagcgc cgcccagagg
     1561 ccgaaccgcg ccgggtcgag gccgacgagg tgcccgacgg ccgggaagag gaggagcgcc
     1621 gtcgcgttca gcgtgaagac cgtcgcgagc gagacggcga tctcgtcgct cttcgccttc
     1681 acgaccgggg ccatcgcggc gatcgcgctc ccgccgcaga tcgccgtccc gaacgagatc
     1741 agcgtcgaga cgggccccgt ggtcccgaag aggcggccga ggaggacccc gagcgcgagc
     1801 gtcagcgcga tcccgacgac ggtgtagagg agcgaggccg cgccgacccg ggcgaccgcc
     1861 ccgaggctca ggccgaagcc gaggccgacg accgacgcct ggaggagcac cttgctccac
     1921 gaggccgtcc gactcggggc ggggttcccg acgaggaggc tgaagaggat gccgcccgcg
     1981 agggccacgg ccgggccgac ccacgggagg aggcacgcgg cggcgagggc gaagaagaga
     2041 gcggcgcggg cgcgcgagga catccgtcca ttattcgccg cgggcgccag gcgaagaagg
     2101 cggaactcgc ccggacgcgg agcctcccgc cgcggggaga gcgggccgca cgaggctgtc
     2161 gcccgcggtg agcagggcgg aggcgagtcc cacaccgatc ccgtcgtgcg cggtccgatc
     2221 ctccccgctg tcagggccgc ccggtaatcc cctcatccac gcgtcatcag cgcgaacacg
     2281 ccccagccca ggaactcccg cgtataggtg gcgtagcgct cgggttccgc ggtgagccgg
     2341 gcccggactt ccttcgcgag ctcgtcgccg ggattggcgt ccagccatcg gcgcatggtg
     2401 agccatttgg ccgcctcgta cctgtcccag ccgtcctggt tcgccaggac catctccacg
     2461 acgtcgtagc cgagccggcc gaaggacgcg aggagctccg gaaggaggag gaagtccgag
     2521 atcgagccgg cgaggcaccc cttcgcgacg tcttccgtcg gcggcagctg gcgccagtac
     2581 ggctcgccga tgaggaggat ccctccgggg cggaggctcc gggccaggag ctcgacggtg
     2641 ccggcgactc ctccgccgat ccaggtggcg ccgacgcagg cggccacgtc caccttctcg
     2701 tcagagacgt agccggcggc atcgccatga aggaacctga cctgatcggc gacgccgagc
     2761 tcctcggcgc ggagcctcgc ctgctcggtg tacagccggc tcaggtcgac gccggtgccg
     2821 aggactccgt agtcgcgcgc ccaggtgcac agcatctccc ccgagccgct gccgaggtcg
     2881 agcacacgcg tccccgtctc caggcgcagc gccgcgccga gagcggcgag cttgtcggac
     2941 gtgaacgggt tgtggatgcg gtgagcgctt tcggtgatgc tgaagatccg agggatgtcc
     3001 aactcggaag acctcctcac gcgtgcgagc gggttcgatc atagccagac gagccgttcg
     3061 acggccggcc gcgacccggg gaccgcctcg gacggagaga gaccggcaaa cgcgacccgc
     3121 cctgccggtg agcttcctcg cacgcccgcg cggcgggtcg gcccgcgggg gggcacggcg
     3181 cctgcgacgc gggggagcgt cgacccggcg ttcgctcccg gcggcgcccg cggcgccgcc
     3241 gggaacgctg cgtcatttcc cgtcgaggcg cttgcgcagg gcgtcgagct ctcgcgagat
     3301 cgccgaggcg agcgagccga tgtccgtctt cctctcgccg aggacgtcgc gggtgaggtc
     3361 cccgatctcc cgctccgcga cggggcggag gatcccctcc gggtccttca tcgtcacgag
     3421 cttcaggacg cggaggaggc gcgtgtagcg gtcgaagaag ggcgcgtcga cgcgcgattc
     3481 gccctgcgcc ttccgcgcca tcgccatcat ctcgccgagg gcggcctcga gcttctcgcc
     3541 gccgctcccc tggcgcgcca tctcctcgaa ctgcacgacg aacctgtcga ggagcgcgta
     3601 gcccgccggg tctccggccg acgccgtgcc gggcgcggac aggaccagga gaaggacggc
     3661 tccgatcagg gctctcttca tcttctgcct cctcgggggc ggacccccgt cttccgtcag
     3721 ctcgctccgg cgatcgcccg cgcgaggcct cccccgcgcg acagctctcc gaggcgccgg
     3781 gccaggtcct cggacggcac cgccttccac tcgaccgggc cgcccggcct tcccccggcg
     3841 aacgccgcca ggaccgtccc cgcgaggtcg tccgggccgc cccggcccga cgcacccgag
     3901 cgccgggcac cgtggacgac cgcctgcacc acccacacga ggtccgccgc gcgcgcgccg
     3961 tcggcctcgg gaatccgcgg ccgcgacggg ctcgcctcct ccgacgccgc gaagaacgtg
     4021 cagccccgga gcgccgcggc gacgatcgcc ggatcgggct ccggagcgtt ccggacgtgc
     4081 cgccacgccg ccagcgaggc ggcaaggagg agcgtcgcga gggccccggc gaggaccggc
     4141 cggagccaca cccgctcggt ccgcgccggc tcggccgcga cggcccggcg cacccgcctc
     4201 gcgaacgcct cgccgtcgaa cgccgcgagg gcagccttct tctccgcgtc gacgagcgct
     4261 cccggctcct cgaacccttc gtccttgctc ggcccgttca tggcctccct ccgtgcttcc
     4321 gtaccgcctc cgcgacccgg gcgcgagccc gatgaagccg cgacatcacc gtgcccggcg
     4381 cgacgccgag cgctccggcg atctgctcgt aggagagctc tccgtactcg cgcatgacga
     4441 ggacgtcgcg aagcggccgg gaaaggtcga ggatcgcctt gcggagcgcg atcctccggc
     4501 ccttgtcctc gagctcggca tccaggtccg tctcgtccgg cgggtccgtc gtcgcgagga
     4561 gcggccggaa gagccccttc cgcgtccgcc gcacgtggtc gaggcaggcg ttccgcgcga
     4621 tccggcagag ccacgccttg accgatcctt cctcccggag ccggcctcgg ctccgccacg
     4681 cccggaggta ggtttcctgg gccagctcct cggcgtcgtg gcggttcctc acgaagccga
     4741 ggcaaacgag gaagacggtc gcgcgatgct cgaggagcgc ttcgacgtcc tcctcggaac
     4801 accgtgtgcc cgtgcgggcc gcctccatgt cgctcatcgg ccgggtccgt ccgcccttcc
     4861 cgccaccgcc acccggcgaa ggcgttctcg ggcatcagga cgaacggcgc gggagtctat
     4921 tccgcgcccc ggccgaaggc tcctcccgtc gcggcgctac ggccgggcgc gctccacgat
     4981 cgcggcgcgg gcgcggtggg ccccctcggt cttcgggtcg gcctcgatga tcctccggta
     5041 gagcgcggcc gcctcctcgg gctcgttccc gaagtcgcgc gtcagcgcag cgagggccgc
     5101 ggcttccccg tcagccacgg cagggtgaga gcggcgttcg ccgagcaggc cctgtcgcgg
     5161 cagatctccc aggtcgggaa cggcagcccg gtcccgttcg cgaagaccac ggccggcacc
     5221 ggcttcccct tcgcccccgg ggggagcgtg acgtccatcg tcctctcccc gtcgccgaaa
     5281 cggaggccag gccgcaggag agcgtcctgc atcccgggga ccttccagac gacgccgtag
     5341 gtcgtcacgt cgtgccgcga gcggggctgc gcgcccgcgg gaagagcgac gacgagcggt
     5401 gcgaaggcaa cgagcgcgag ccgagcggca atcctcatgc tcccgacctc ctgatcccgg
     5461 ttcatacgca cccgacccga cgaggttccg gcgagcgccg gtgcgcctcg tcgagacgtc
     5521 cctccgcccc ggagcggtcc accggcggga ccgtctgcgc ttcagcccgc gagaaggagg
     5581 acgaggacct ccgccgagac gatccggagg atcatcgtga gcgggtagac ggcgacgtag
     5641 ccgagcgtcg gcgcgtcgga gccggcgagg ccgttcgcga aggcgagggc tggcgggtcg
     5701 gtcatgctgc cggccatcac gcccgtcagc gtcaggaagt tgacgcggaa gacgagcctc
     5761 ccgaccgcgc cgacgacgag catcgggacg atcgtgatcc ccgccccgat cgccatccag
     5821 gtcagccccg gccccttcac gagcgtgtcg acgaagcccg cgccggagcg gaggccgacg
     5881 gccgcgagga agagcgcgat gccgatctcg cggagcatga agttcgcgct gatcgggagc
     5941 cagaagacga gcggcccgac ccgcccgagc cgcgagagga ggatcgcgac gaggagcggc
     6001 ccgcccgcga ggcccagctt caccggcgcc ggcatcccct gcaggtggat cgggaagagg
     6061 ccaacggcga cgccgagggc gatcgtcagg aagacgggga cgaggtgcgg gtagttcagg
     6121 tcgcgcggcg agtcaccgag ctcggcggcc accttccgga tctcctccgg gccgccgacg
     6181 gcgaggatcg cgtcgcccgt ccggagctcg aagccgggga aggcagccat ctccagctcg
     6241 gagcgcgaga cgcgggtgat ccgcacgccg aagcgggccg cgaagttcag ctcgccgagc
     6301 gtcttcccga cgacctcctt ccgcgtgacg atgagccgct tgctcgtgat cggggtcggc
     6361 aggctccgca ggtcgaggtc gctctccttg ccgacgagga gcctcaggtc gtcgaggtcc
     6421 ttcttcgccc cgaccgcgag gaggacgtcg ccgacggcga ggcgcgtctc gtgcgtcggg
     6481 acgaggagct cgccgccgtg gtagacgcgc gagatgacga ggccggcgcg gtcgacgagc
     6541 gggagcttct cgagaagcag gccgtccagg ttcgcgttcg tcacctcgag gttcgcgcgg
     6601 tggagcggcg gcgcctcgct ccggcgggag cgctcgagga gcctctcctc gagcggcatc
     6661 gagacgcgga acgcgacctt cagcgcgagg atcgcgagga tgatcccgag gacgccgaac
     6721 gggtaggcga ccgcgtagcc gagcggcggg atcgtcgcgc cgcgggcgag cgaggccggg
     6781 tcggtcgcga cctccctcag agcctgctgg gccgcggcga ggctcggggt gttcgtcgtc
     6841 gcgcccgaga ggatgccgac gacggccggc agctcgacgt cgaggaggag gtgcgccgcg
     6901 gcggccagac cgacgccgag gaggacgacc gccgtcgcga gcaggttcag cgtcatcccg
     6961 ctccgccgga gcgaggagac gaagccgggc ccgacctgca ggccaatcga cgtgacgaag
     7021 aggatgaggc cgaagtcccg cgcgaactcc aggacttccg gctcgagggc gacgccgaag
     7081 tggccgagga ggaggccgac gaagaggacg cccgccgtcg tgaggccgac accccgcacc
     7141 ttcactgagc cgagggcgag cccgaggccg gcgacgaggg cgaggacgag ggccgccggg
     7201 gccccgggga ggagcatcgg cggattctcg cgcggcgcaa atgccgcgtc catccggccg
     7261 gccccgctcc gcccgagggt cccgcgaggg acccgtcgag gggagggcct tctgcatcga
     7321 ccgaaccttc tcgagctcgt cgatccggct ttcgaggcgg cgggtcttcc tcatccgcgg
     7381 gtcggcgatc tcctccacgc ggacaccgca gacgacaccg tgacgagcgc tgttcgggtg
     7441 ggagggccgg cgcctccgcg aagaacgcct cgaggtcgca gcccggagga cctgccgccg
     7501 caacccctcc tggccggact ccgtcagccc gcggatgacc cggtccacct tctccttcga
     7561 gcggggcttc cgttcggcca tctgcacgta cagcgggcag acgttcgtgg cgttcgtggc
     7621 gaagacgcag cccatcgtca tcgtgacgcc tcgaatcggg gcgtggggct tcgtccgcgt
     7681 caggcgctcg gcgggtagcg aaagtgaccg gtgatgacgt acgagaagag gacgtcctcc
     7741 tcgcgagccc cggagccgat gttgtggagg acgaggggtg tgccttgcgg cgtgcgacgg
     7801 tccgagacga agccgatgtg cggcacgccg ctcgcgaggc gccagacgac gatgtcgccc
     7861 ggggagaagc ccgcggcgcg tcgggagacc ggcagcgtgg cgccgtggcg gcggaagaag
     7921 gtgacgaggt tcgggacgcg ccgatggtcg atgctcggat cgggacggga gaggcgccag
     7981 agcttcggat aggcggacca ggccgccttc atgtcctggt ggacgagtac ctggaggtcg
     8041 attccgaggt gacggtaggc gcgcacgacg acgtccgtgc agacgcccgt ctgcgcgggg
     8101 acgtcgcccg cgggatacgc gagcctccga tacgcgccgt cgtacgaggt cgtgacgccg
     8161 acctgcgcgc gggcggccgc caccagccgc tctccggtcg acggcggcgc ggtgggcgtc
     8221 gcgccgagga ggagcaggag gaggaggctc atgagtccgg gggcgcctga cacggcgggt
     8281 caggcgcgaa cgaagcgagg gccccgggcg ctttcccgct ccggcgctcg gagcgggtca
     8341 cggacgccgg cccctccggt agacgccgca cgagggcgcc ctcagagaag gcgctcgagc
     8401 gcggagacga ccgtccgcac gcacggctgg cagacgcgga cagggaggcc ggaggcgtgg
     8461 aaccgctcct ggccctcggg cgtcgagagg tcgcagccgt cgagcagctc gcggcagacg
     8521 tgcgtgccgt gcgcgtcctc gaaggcggcc acgagctccc tcaccttcac gtaggtgtcg
     8581 gccgtccggg ccttgtcctg gttctcgccg cgcccgtgct tcagtccgag ggcgatcacg
     8641 ccgccgctca aggcgccgca gatctcctgt tgccgggcga tgcctccgcc gaacccgcag
     8701 gcgagccgga gagcgtggtc ggcgtcgatc ccgaggtcgg gcgcaaacgc gacgagcacg
     8761 gcctgggcgc agttgtagcc gttcgcgaac ctgtcgacgg cggtctcctg gtgggtcgtc
     8821 atggcctccc tttcgtcggg gcgcgagctc ccgcgtcgcg cggtgtggaa tcggagcggt
     8881 cacccgggag cgatccggac cgcgacgtag agcttccgga tcggtcgccc cttcaggcga
     8941 cgcatcacgc ggacctcctc gagccgcagc cccgccgcgc gcaggagctg ccggatctcg
     9001 gagtcctcgt acgagcgctc tttcaccgcc accaggaccc gacggtacag tttcgcctgc
     9061 ctcacgaacc agagctggcg gaacgtcgcg acgcccgtct tccggtcgaa tgcgttgaag
     9121 gcggtgaaga cgtgcggccc ggcccggcag agcttctcgc tcgtgctcag ccactcgagg
     9181 aagaagcgcg tgttgaggtc gaacaggaac aggcctcccg gtcggagcag gcgcgcggtg
     9241 ttcgagaaga cccgctggag atccttcgcg tcgaggacgt ggttcagcgc gtcgaagtgg
     9301 cacgtcgcga cgtccgcgga aacgtccagc gaaagggagg tcaggtcctg cctcacccac
     9361 cggacgcgcc gggacttctt ccgcgcgacg cggagcatag cctccgacac gtcaacgccg
     9421 atcacggtgc gcgcgcgcgc gcgggcccac ggaatggccg tgttgcccgt gccgcaggcg
     9481 aggtcgacga tcacgccggg gcggaggccg cgcgcggccg ccgcgtccgt cagggctcgc
     9541 cggagctccc ggtagaggcc ccggatggcc ggatcggcgg ccatccggtc gtagagcgga
     9601 gcgagctgtc cgtacgggtc cgtcatgagc acctcggtcc attctcgacc tggtctgtgg
     9661 gcagcgtccc gatgaacggt ctcgctccgg ccgcccaggg acgcgcgccc ggcagaaggg
     9721 gacacgggac gggtcgcgac aaatcacgtc ctcccatcgg gtctcgcgcg gacgcgcaaa
     9781 cgagatgccg gccaggccgt gagagagggc cgcaggcggt gccggaggtc ggcgcacgcg
     9841 gtccgcggga accgcccggc ggagcgacga aggcgcaccc cgcgcctccc tcgccatccc
     9901 ggaccgagac ggtccgcaag ggcgttcgga gcttcctgtc ggggccgcga cggactagag
     9961 ttcgccgatg cgcctcgctc cgctcgccgc cctcgctctc ctcggcctcg ggctcacccc
    10021 tgccgccgtc gccgaaggcc cgttccggcc catcgattcg cccgcggaag cggccgggct
    10081 tccacagacg acgattcgaa gcaccgccgt ccgccccttc gccttcgacg cccggctgtt
    10141 tgcggtcgtc atgagaaccg tggcctccga ggctccgagc gaggtcggga caccttcgac
    10201 tcttctcgag ctgccgcatc cggatggggg cctggagcag ttccgggtca tcgagtcgcc
    10261 cgtcatggcg cccgagctcg ccacgaagtt ccctgagatc cggacgtatc gcggcgtcgg
    10321 cgtcgacgac ccgaccgcct ccgtccggtt cgaggtctcg ccgcgtggct tctcggcgat
    10381 ggtcctctcg gccgccggcg cctggtacgt cgacccctgg acgaagggcg tcgtcgacgt
    10441 cgtcggctcg taccaccggc gcgacgcgtt gcgcggcgag gggagcccgt tcgagtgcca
    10501 ggctcccggc acacccgacg acggcgaggg acccggcacc cacgaggcga cgacggacct
    10561 cggaccgacg cccttcccgc tcgcctccgt cggcccgacg ctccggacct atcggctcgc
    10621 gctcgcgacg accggggagt actccgtcgc ggtctgcgga tccaagccga cgacggcgtg
    10681 cgtcgccgcg gagctcgtcg tcgcggtgaa ccgggtgacg gggatctacg agcgcgaagt
    10741 cgccgtgaga atgacgctca tcgcgaacaa cgacctcctc atctatctcg acggcgcgac
    10801 cgacccctat acgaacagca acggcggcac gatgctcggg cagaaccagt cgaacctcga
    10861 ctccgtcatc gggagcgcga actacgactt cgggcacgtc ttctcgaccg gcggcggcgg
    10921 ggtcgcgtac ctcggggtca tctgcgaatc cggctggaag ggcggcggcg tgacgggcca
    10981 gtcgaacccg accggagacg ccttctgggt cgactacgtg gcccacgaga tggggcacca
    11041 gttcggtgga aaccacacgt tcaacgggag cctcggctcc tgcaccggag gcaaccggaa
    11101 cgggccgacc gcgtacgagc cggggagcgg gtcgacgatc atggcgtacg cgggaatctg
    11161 cgggttgaat gaccttcaac cgcacagcga cccgtacttc cacgccaaga gcttcgacga
    11221 gatcaccagc tacgtttcgg gaggcggcgc ctcctgcgcc gtgagctcgg cgacctcgaa
    11281 ctccgccccg gcggccgacg ccggcgccgc gtacacgatc ccctcccgga cacccttcgc
    11341 cctcaccggc tcggcgacgg atcccgaggg tgatgccctg acgtacggct gggagcagct
    11401 cgaccgcggc accgtggagc cggcaccgcc gctcgtcgac gccggcaacc gccccatctt
    11461 ccgctcgttc aacccgacgg tctcgccgac ccggatcttc ccgaagctcg ccgacgtcct
    11521 ctccgggacg attacctacg gcgagacgat gccgacgacg agccggacga tgaacttccg
    11581 cctgaccgcc cgcgacaacc gcggcggcgg gggcggcgtc gcctccgacg cgacgacggt
    11641 caccacggtc agcgcggccg ggcccttcgc cgtcaccgct cccgcgtcgg ccgtgatctg
    11701 ggccggcggc tccacgcaga ccgtcagctg gaacgtcgcg gggacgacgg cttcgccgat
    11761 ctcctgcgcg aacgtgtcga tcgccctctc cacggacggc ggtcagacct tcccggcgac
    11821 cctcctcgcc tcgaccccga acgacggctc ggagacggtc accgtcccgg acacgccgac
    11881 gaccggagcg acggcgcgag tccgcgtcgc ctgcgtcggg aacgtcttct tcgcgatctc
    11941 gcggccgccc ttcacgatcg ggaccccgcc ccctcccccg acggtgtcga cggtcctccc
    12001 gtcctcgggc cccacggccg gcgggacgac cgtgacgatc accgggacga acctcggcag
    12061 cacgagcgcc gtcacgttcg gcggcacggc cgccacctcg ttcacggtga gctcggcgac
    12121 gcaggtgacg gcgacgacgc ccgcgcacgc ggccggagcg gtggacgtcg tcgtcacgac
    12181 ctccggcggc tccgcgacgt cgacgggcgg gttcacgttc gtcgccccgg cgccgacgat
    12241 cgcgtcggtt gcaccgagct cggggtcgac cctgggcggg acgaccgtca ccatcaccgg
    12301 ggcaaacctc accggcgctt cggccgcgac gttcggcgga gcggcggcga cctcgctgac
    12361 ggtgatctcc gcggcccagg tccgcgtgac gactccggcg cacgccgcgg gagcggtcga
    12421 cgtcgccgtc acgacacccg gcggcaccgc gacgaagtcg gccgggttca cgttcgtcgt
    12481 cccgaagccg acgatcgccg cggtcgcgcc cgcttccgga tcgacgctgg gcggaacggt
    12541 cgtgacgatc acgggaacga acctcagcgg cgccagcgcc gtcaccttcg gcggcacgcc
    12601 cgccgcgtcg ttcaccgtcg actcggcggc ccaggtcacc gcgacgacgc ccgcccacgt
    12661 cgcgggagag gtcaacgtcg ccgtcacgac gcccgggggg accgcgacgc ggacgaacgg
    12721 gttcacgtac gtctccgtcg tcccggcgat cgcgacggtc gcgccggccg aaggcccggc
    12781 ggctggcggg agctccgtca cgatcacggg gacgaacctg acgggcgcca ccgccgtgag
    12841 ctttggcggc agcgccgcca cctcgttcac cgtcgactcc gcgacgcaga tccgcgccac
    12901 gaccccggct cacgctgccg gcgcggtcga cgtcaccgtc acgaccccct acggagacgc
    12961 gacgtcgacg aacggcttca cctatctcgg cgcgcccaca gcgctccggt tccacacggt
    13021 cacgccctgc cggatcgtcg acacgcgctg ggagcccgac gggccccttg cgggtccggc
    13081 cctccaggcc tcgccagcgg agcggacgtt cgcgctcgtc cccggctgcg gcgtcccggc
    13141 cgatgcgctg gtcgtctcgg cgaacgtcac ggtcaccgga gcgacggcgg ccggcgcgct
    13201 ccgcgtcttc gagccgggct cggccccgac cgaggcgacg atcatctcct tcccggccgg
    13261 gaggacccgc gcgaacaacg cgctgatcct cgtctccggc tcgggagagg tcaccgtccg
    13321 gaacgacgcc gacggcgagg tccacctcat catcgacgtg aacggctact tccggtagcc
    13381 cgcgcccacc ctggcgcgac gaagcggccg gcgcgagccg gccgcttcgc tttccaaccg
    13441 ggccctgtcc ggcttacgcc ctccggaacc agagcgactg ctgcgcgacg ggggccgcct
    13501 tcgcctcgag ggcggcgagc tgcgcccccg agagcggcgt gaaggccttc gcgatcgcga
    13561 cgttctcctc gagctgggga atcgagtcgc agccgacgat gacggtcgag acggggagcg
    13621 tcaggacgta gcgcatcgcc tcggccatcg agagggtccc cgtccgcgtc gccttcgcgc
    13681 gcccgcgctg ctcctcgagc ggcggcggga cgtacccggc gaggaggcgc ccccgcgccg
    13741 ggaccttcat cccgacgatc cccatctcct tctccacggc gagcgggagg agctcgctcg
    13801 cgaacgggag gtgatgcggg tcggcggcgt tgaccgcgag gaggacggtg tcgaacggga
    13861 agcggcggat cgcctcggcg aggacggccg ggtcggtgtg gcccgacagg ccgaggaagc
    13921 ggacgagctt ctgctcgcgc gcctcgcgga acgcctcgag ggcgcccccc ttcgccgtca
    13981 ccttctcgac gtcctccatc gtcgacatcg cgtggagctg ccagaggtcg acgtggtccg
    14041 tccgaaggag cttcagcgac gtctcgagga gcttcagcga gccgtcacgc ccgcggtcgt
    14101 gggtcttcgt ggccacgaag acctcgctcc gcctcttcgg gagcacctgc ccgaagtagc
    14161 gctcgctcca gcgcgcctcg ccgccgtagc gggcggaggt gtcgacgtag ttcacgccga
    14221 gatcgagcgc ccgctcgatg agcggcaccg ccacggcgtc gttctccgcc ttctcgatcg
    14281 cggcctgccc gccgaggctg aagatgccga cgcgaaagcc ggtcttcccg aggttgcggg
    14341 tcggcatcgc gtcggcggtg cgcgggttcc agggatggcc cgaagctccc gtcgcggcgg
    14401 gcttcgtcgc cgcgtcgacg gcgggctcgg ccgagaggcg ccccgcgacg agccccgcgg
    14461 cggcggccgc cccgagcttc aggaggtcgc ggcgctccac gccgccggtc gtcgtcccgt
    14521 cgcgctccgt catcaccgca cctcccgccg gggctccggc ggccgaagta tggcgcgtct
    14581 cgcggacgcg aatcgatggg tcccgaggaa cgccgtcccc tcgacgctca gcgcctcccc
    14641 ccgtccggcg cggtcgccct ctcccacgcg aggcggcgaa ggccggggtc ctcggccttt
    14701 tccgcgacct cgcggaagat cgggagcgcc tcggcgaggc gcccggcgtt caggagcgcg
    14761 atcgcgtcgt tgtagcgacg gacgagccgg ttgtggacga cgacgcggcg cacctccgcg
    14821 acgtcccagc cgagcgcgcg ctgcatctcc gggtcgcgcc cgagggaggc gtggagcgcc
    14881 tcgagacgcg cgagcgcctc ctcctcgcgc cccgcgtcga tgagccggtt cacgaggtcg
    14941 acctcggccg gcacgtcgcg ggagagggac ggggacgacg ggcgccgcgc ggcggaggag
    15001 gacggagcgg gcggctcctg aaggtttgcg gccggctcct ccgcggggcg ccccttcgcg
    15061 acggcgcccc cgaggagccc cgcggcgccc gtgcgcgtca ggggcaggac gcgccccggc
    15121 ggcgccgaag cgaccggaag gaaccgagcg agagagcggc cggcgacggc gtgggcacgc
    15181 gcacggagga ggaggcttcg ctccgtatca ccgaacgcct ccgcgaacgc cgtcccggcg
    15241 tcgcgcccct cccccaggag cgccgcatgg cgagcgaggc gctcgccgcc gtccggcgag
    15301 gcgtggagaa ggtcgtggac gacggcccac gcctcggcgc ggaggagggc ggctgccggt
    15361 ccgtcgcccg agagcgacgg ggcgacgaag agcgccggcc ggccggccgg cggcccggcg
    15421 gcgcggagga gcgagacgtg ccgcgggacg agacggccga gcgtggcgcg agcgcccgag
    15481 acggaggcgg tcgaaaggta ctcggcgagc cccgtcgcgg cccactccgg gagcgcgggg
    15541 cgcgagcgtc gcgcgagcgc gacgaccgcg gcgcgaacga gcgcttcgcg cgcctcctcg
    15601 ccgcctgcgg ccgcgaggac gatccggccc cccgcgcggg agggaagcga gattcgcgag
    15661 aggtgctccg gctcgtgcgc gagggcgacg acgagcggcg gcggtgcacc gaacgccccg
    15721 gggacgatct cgggaaggag ggcggtgagc gccgcgagcg tcgccgccgt ctcgacgagc
    15781 cgcggatcgg aggcggtccc gacgaccgtg accggccccg cttcgacctt ccgccacgac
    15841 tcccggacga cggcgggccg gggcgcagcc ccttccgcgg ccgccgccag caggagcgcg
    15901 aggaggggcg cgagcgggcg gagcctccgg acccgcccgg gccgcggcct cccgctcgcg
    15961 agcgagtgcg cgcctgcttc gtccaccgcg ccgagtatgc ggcccgcgac cggcttcccg
    16021 cgcgccggcc acgtccgccc ggggccgtcc ggcgtagagc ggtgcgatga cgacgacgcc
    16081 cgaccacccc gtccgcccgg ccgccgccgc gctcctcgcg acggcggcga cgttcctcgc
    16141 cctcgacctc ctctggctcg gcgtcgtcgc ccgcggcctc tacgagcggg cgctcgggga
    16201 cctcctccgg cccgacgcgt accttccggc cgcggctctc ttctacgcct tctacgtcgc
    16261 cgtcaccgcc ggctgggccg tcctcggcgc ccgttcgaag ggagacgccg cccggcgcgg
    16321 cgccggcctg gggttcgtcg cctacgcgac gttcgagctg acgagctggg cggtgatccg
    16381 cggctggccg gcgctcctcg tcccgatcga cctcgcctgg ggcgtctccc tcacggcgct
    16441 cgccgccctc gccggccgcg ccgcgcacga cgccctgcgg gggcggcgat gagcctcgag
    16501 ctcgtcgtcg gcaacgccgc gctcgtcctc gcgctcatga cggtcctctg ggtcgcgagc
    16561 gtccggctcc gcgacgcgag cctcgtcgac ccgtggtggt cgatcgcgtt cctcctcgtc
    16621 gccgcgcaca ccgcccttcg caccggcctc acgcccggca aggcgctcct cctcgcggcg
    16681 gtcgcggcct ggtcgctccg cctcttcctc cacctcttcc tccggagcct cgggaagccc
    16741 gaggaccccc gctacgccgc gttccgcgcg aagtacgggc cggagcggta cgggtggttc
    16801 agcttcttcc aggtcttcct tctccagggg gcgctcgtcg tcgtcgtctc ggcgccgctc
    16861 caggtcgcgg gggccgcccc cgcgcccgac ccgatctcgg tcaccgacct cctcggcctc
    16921 gccctcttcc tcgccggctt cgggatcgaa gccgttgccg acgcgcagct cgcccgctac
    16981 cgaagggccc gcgcgaaggg cgtccctagc gcccccggcc cggtgctcga caccggcctc
    17041 tggcgattca gccgccaccc gaactacttc ggcgacgcgc tcctctggtg gggtttctgg
    17101 ctcttcgcgc tcgacgcggc gtgggggtgg gcgacggtct tcgccccggc gctcatgacc
    17161 ttcctcctcg tgaaggtctc cggcgtcgcg atgctcgacg cccacatggc ggcgaagaag
    17221 ccggagtacg ccgagtacat gcgccggacc tcgggcttcg tcccgcgtcc gcgtcgcgcc
    17281 tgagggaggg ctccgctctc ccggctcgtc gggcgtcctc acccgacacg cgcgagcaga
    17341 cagcgctcgt ccgagcgggc ccgccgctcc ccgacgcgcc gtagaatcgt ccggatcggg
    17401 gccgcgcacc gtgcaggccc gggcgcgact ccggcgcgga gggtgtcatg agcggtcgcg
    17461 cgcggcctcg tcgtggtctc cggctcctcc tcctcgcctg cgtcgccgtc ggcatcacgg
    17521 cgacgctctc ccacggccat cccggaccgg ggcccgcgac ccgtgcggtc gtggcaagcg
    17581 gtaccgtcgt ctacatcccc gcggcggccc acgcgagcgg ggcgaacggc gcgagctgga
    17641 ggaccgacct cgaggtccac aacccgggcg gagcgacggc ctcgttcacg atccagctcc
    17701 ttcggcgcga cgccgacaac tccaccccgg cctcgcggcc gtacacgctc ggcgcgcagc
    17761 ggtcgcgccg cttcgtcgac gtcctcgtct ccgagttcgc gacggagggg gcggcggcgc
    17821 tccggatcgc ggtgacctcg gggtcgatcc tcgtgacgag ccggacgtac aacctgatcg
    17881 gagagaaccc ctggaagctc ccgaaggggg cgtccttcgg ccagtacgtt cccggatggg
    17941 aggagaccgc ggcgatcggc tacgggcagg aggggcggct cgtccacctg acccagcagc
    18001 ccgcggcgag cctcgcgggc tttcggacga acctcggcct cgtgaacgcg acggcgagcc
    18061 cgatcgccgt ccgcgtcgac ctctatcggg ccgacgggac ctggctcggc aagaaggacg
    18121 gcgccgacac ctccctgccg gcgtacggct tccgccagct ggatcaggtc ttctccgcgt
    18181 tcggcaccgc ggcggacggc tgggcgctcg tgaaaacgac gacccccggg gcgaagctgc
    18241 tggcgttcgc gacgctcatc gacaaccaca actcgggcga cccggtcttc atcccggcgg
    18301 agcggaccgc gccggtcgcg gcgccgacgc cggggccgac cgcgaccccg acgcgcaccc
    18361 ccacccgtac accgacgccc actccgaccc gaacgccgac gcccgggccg actcccggcg
    18421 cgaggccgaa cctcgtcttc tacgtaccga cggcgtggcc cgcctgcttc gtcgtccact
    18481 ccgcgtccgg ctgctgcacg agctccggct gctgcacccc ctcgccctcg acgacgggga
    18541 cctcctacat ccagatgttc ctcgcgaaca tcggcagcgc cacggcttcc gggccgatcc
    18601 gcttccgcct gtccatcgac ggcgtgccgg cagggacctc aatctgggac aacgccactg
    18661 gcctcgagtt cgggctcggc gtgatcctca cgtgggagta cacggggacg atcgtgcccg
    18721 ggatccacga cgcgacgatc accatcgacc ccgacgacac gatcgccgag tcgaacgagg
    18781 ccgacaactc ctgcacgacg acggtgaact ggggcggcgc gcccctgttc gtcggctccc
    18841 cggcgcggga tgcgagcgtc gacgccctct ccggcggagc ggccgttccc ctcgcccgga
    18901 gcgccccgcc ggagccgccc ggttcggcag cgctcgagcc ttcgacgggc ccgatctacg
    18961 tcccggcggc cgcccacgcc tccggcgtga acggcgcgag ctggaggacc gatctcgagg
    19021 tccacaaccc gggggcgacg gccgtcacgt tcgacgtggc gctcctgcgg cagaacgcgg
    19081 acaacggcgg gtcccccacg acgaagagct actccctcgg gccgcagcag tccgtccgct
    19141 acgcggacgc cctcgcgagc ctcttcggat tcaccggagc cgccgcgctc cggctctcgt
    19201 cctcgccggg cccgatcctc gtcaacagca ggacctacaa cctgatcggg cccaacaccg
    19261 tgaaccttcc ggtggacgcc tccttcgggc agttcgtgcc gggccttccc gaggcggagg
    19321 cgatcggcta cggcgaggag gggcggctca tccagctctc gcaccgcgac tcctcggcgc
    19381 tcaccggctt ccggacgaac atcgggttcg tcaacacgac ggggagcccg atcgacctga
    19441 aggtggacct ctacacggcg gacggaacgt tcctcgggac cgtccaggac gcggcgacgc
    19501 accttccgcc gtgggggttc gcccaggtca acgccgtctt cgcgcccttc tcggcgtcgc
    19561 tcgaggacgg gtacgcggtc gtcagaacct cgacgccggg aggccgcttc ttcgcgttcg
    19621 cgacggtcat cgacaaccac ctgacgggcg acccgatctt cgtcccggcg cagcgggtga
    19681 cctcggggtg ggtgaccgcg gggagcggca cgatcggttc cgcgggcggc accgtgagcg
    19741 gcggcgggct caccgtgacc gtcccgagcg gcacgttctc gcaggcgacg acgctgacga
    19801 tcgccaggaa cccggctgcc gcgacgccgc acgaccgcta ccggacgacc gacgtcttcc
    19861 gcctcgacgg gctgccggac gagcgcgcga aggcggtgac gctcaccctc gacgtgacgc
    19921 gctcgcccgg cctctccgga aaggatttcg tggcggcggc aaacgaggtc ttcgtcccca
    19981 ccggggcgtc gctcggcgcg ccgccgatgc tcctcgcggc gacccgcgcc gcgagccggg
    20041 tctcggcaac gatcccgccg gcgacggccc cggcggccag cggcctctcc ggcgccgccg
    20101 cgcccgcgct ggccgcggac gtccggccgc agttcacgat ctgggggatc gcgggatacg
    20161 cggagctcct gagcgcgcag ggccacttcc gggtcaacta ccccgtcacg gacctggtcc
    20221 tcggaagcgc ggaggagatc gcccaggggc tcgaggcggc ctacacgaaa atcgccgcgc
    20281 tcggattcga atggtcccgg cggacgtcct ggccggtcta cgtctcgatc gagccgtttc
    20341 cggccgagac gaccgcgcgc ctcgccgagt gcgtcccctc gcgatggggc gtgaactcct
    20401 actggctcca cctgaacgcg gaccgcctga acgcacaggc cgacatcccg ctcatgaagg
    20461 acgccgcggc ccacgagctc ttccacctcg tgcagtacct ctacgacccg cgcaaccgga
    20521 tcaccatggc cactcgcccc ggcgcgtggt actgggccga cgaggccacg gccgtctggt
    20581 tcgagaacca cttcaacggc tcctccgccc cgccgcaggt cgtgcgggag aaccatgcct
    20641 tcatgacgac gagggggctc gagttcccgc cgccaggcga cgacccggcg cttgcagggc
    20701 cggtgcagga ccacgggtac ggagccgccc agctgatgcg ggacctggcg aaacgaaaag
    20761 gggacggcgc tgtcggcgag ctcgtccggc ggaaaggaaa ctcgtccgtc ctgcccgtgg
    20821 acgcctacga cctacagacg ggagcgaacg ccgagagcac gtggcgcctc ttcgtcgacg
    20881 agtacgccca gaagcacctc tacggcgcgt tcccgaccgg cgcagacgtc tgggtccgca
    20941 cggcggggaa ccggtacgcc ttcgcctccg cctcgaccac gggaacgacg ttcaactgga
    21001 cggcgcccga cctctcggcc cgcttctacg gcatccagtt cacggacaag acgtggccgg
    21061 ccggcacgga ggtctccctc ctcctgacgg acacggagaa ggacgccatc tcgctcgtct
    21121 accgcctgaa gtcgggccag ctcacgaggc tggggtacca cctcgacgga gacgtcttcg
    21181 aggtcgagaa cgccgaaacg ttcgctaacg acggcacgag cctcgtgatc ctcgtcgcca
    21241 acgggcgcgc ctcccgcccc tacaccggca cggccccgat cagcctgagc gtctcggcgg
    21301 gcgcgcggaa cgagctggac ttccgcgtga cggggacgga gtgccccgga tacacgcagc
    21361 tctccatgag cttcaagagc gcgctcgacg cgtacgacct caggtggacg ggcaacacgg
    21421 tcgaagtcgg ctggagcggg agcctctacc cggacaacca gagcgagtac tcgggcgtgg
    21481 ccaccctctc cggggatcgg aagaccctca cctccctcgt gttcacctcg cactcggtca
    21541 ccacgatgcc tcccggaacg acgtgtggcc catcggggtg ccggatggag agctgggagt
    21601 cgatcacgtg gtccggcgtt cctccggcgg gggactggca gccctcgcag tttcacttcg
    21661 cgacagcggg cgcggcggca cgcgcctaca tcaccgcgtt ctcggcgcgg agcaggtgga
    21721 ccggcttcac gctcgcctcg cagaaccacg actgcacgta cgacatcgac gaggcgaaga
    21781 cgggcgagac ccggttcgac ctctggctga cgcccccctg accggcgact acgccccgtc
    21841 gcggctgacg gtcgccgcgg tcgacatcag ctcctcgagg agcgcgatcg ccgccttgcc
    21901 gctcatcgcg aacgggtcga ggaccgcggt ctcggagtac cggaggaccg tcccgaggtt
    21961 cacgcggttc agccggacgt tccccatcct ccacgtcgcg aagcgccgct cggagatctc
    22021 cgtgtagtcg aggagcgtga cgtccctgtg gcgcgggtcg cgcaggatcc ggccgtagag
    22081 gtcgttcacc gcggcgcgcc ccccctcgag gacctggagg aagacgttcc cctcggcgga
    22141 cgtgcagagg attccggtga tgccgtgggc ggcgttgtgc gcgtgcgcct tctcgaggat
    22201 cgaggcgacg agcgcctcgt cgatcggggc gagggcgcgg ctggcgtaga ggagccggac
    22261 gagcatctcg agcctcccta cttcatccgt cccgcgagga gcgagaggaa ctcgcggcgg
    22321 aggtcggggt tcttcaggaa cgagccgcgc atgatgctgt tcgtcatctt cgagtccgcg
    22381 tccttcacgc cgcgccactt catgcagtag tggtcggcct ccatgacgac ggcaaggccg
    22441 tcgggctgca tcttgtcgag gaggagctcg gcgagctccg tgatcgcctc ctcctggatc
    22501 tgggggcggg ccatcaccca ctcgacgagg cgggcgtact tcgagaggcc gatgagggcg
    22561 gagtgctcgt tcggcatgac gccgacccag acgcgcccga tgatcgggca gaggtgatgc
    22621 gagcaggcgc tccgcaccga gatcggcccg acgatcatca gctcgttcag ccggccgacg
    22681 ttcgggaact ccgtcaccgg aggctgctgg acgtagcgcc ccgcgaagac ctccgtgagg
    22741 tacatcttcg cgacgcgccg ggcggagtcc ctcgtgttgt ggtcgctcgc ggtgtcgatg
    22801 acgagggcct ggagcacctc ctccatcttc gccgcgacct cctcgcggag gaggtcgatc
    22861 tccccgtcct cgatccaggc ggcgatgttg tcgttcgcgc ggaagcggcc gcgtgccttc
    22921 cggatgcgct cccggatccg gtcggcgacg ggcgtccctc cgccggaggt gcggctcgcg
    22981 ggctctgaaa tcccggcctg gctcgacatg gggtctctcc tgtccgaaag cctaccaccc
    23041 gaccgcgggc cagaagccgt ggaagccgat cgggacctgt cgctcgaagt cgatccgggg
    23101 ccactcgaag ctccccggcg cgagcacctc gttccgcgcc tgcgtaggcc ccgacgccac
    23161 gcggatgcgc cggaccgagc ccgggcgctc gaagaggcct cggtcacgaa acgtgattcg
    23221 gcgccgcccg caggtcccgc ggcaggatca tcagcacgct cccgcctgcg cccttccagc
    23281 ggagcgagtc gacgatgctg cggggggcgc cttcgaagga gtccgcgtcg tcgcgccggg
    23341 tggaggcgac gtcctccgtc tcaccgggcc acgacgaggc ccgcggcctt cgcggtcacc
    23401 tcgacacggg cgggctggag gcgtccctcg agccagacga cccgctggga cttcaggtcg
    23461 tcccggagga cgccgaaggc ctcgccgagc cgctcgatcg tcgaggcgtc gacggctcca
    23521 ggccagtccg cttcggccgg aggctctgcg ggcgcgagtc tccagacgcg aagggggacc
    23581 cgcagccgcg cgaggtaccg tcggacggtc ggggcgtcga tgtcgcccgt gtcgcgcgcg
    23641 ccggcgccga ggaggaggac gacggctcgc cgacgctcgc gccgggtcgc ggagagaccg
    23701 gcggtggcga ccgcctcggc gatccgcgga ggcgtcggcg gctcgttcgc gaagggcgtc
    23761 gccccgacct cgaggaagcc tccgcctcgg gaggggtagt ccctggagat cgggaagagg
    23821 cgcgtcacgg cgttcggacc gacgctcgcg cgcggattcg aggagaggaa gaggaaccgg
    23881 gagtcctttc gcaggtcgac gggccgcgct cccggcgggg cctggaagct ctcgacgagg
    23941 aggcgctcga agtccgggcc gatcgtcccc gcctgcacga agacgacctc ggcctcgcct
    24001 tcctcgacgg cgacgaccga gagcgcccgc ccgttcgact cgaaccagcc gtgcatcccc
    24061 tcgacccgtc tcgggaggcg gaccccgcgc aggatgcgga tcgggacggc ggagagctcc
    24121 cgcaccgact ccttcttcgt ccgtccgccg aaagccgtga ccgccgtcgc gacgattcct
    24181 cccgggaagg tcaggtcggc gatgaggacg tgggcggtgt ccggggagcg ggaagggatc
    24241 cggatccggg cggggtcgct cgcctcgagc ggcattccgt cgaaggtgac cgcgatcgac
    24301 gccgggtcgg ggctgacggg gcagcgccag aagaggcgcg ccgccgacac gcggccggcc
    24361 ccgtcgcgct cgagggcgaa accggcctcc gcttccggcc gggcgcggtt gatccactgt
    24421 gtcgcctccc cgacgcgctt cccggcggcg tcgtacgcga ccgcgacgag ctcgcggggc
    24481 gcgagctccc gaccgaggtc gagcgtggcc acccagggcg ggcccaggct cgcgaccgtc
    24541 gatccgtcga gggagagctc gatgcgggcg acgcccggcc cggccgagag ctcgacggcc
    24601 tgcgggccca cgacgatgcc gaggaagagg gtcaggaacg caatcactcg agtcggcctc
    24661 tcggcgagcg gatgcacgag tcgtgctcga ggggcgtccg ctcggggaga ctcccggcgc
    24721 acgtttcgct gcgccgccgg ggcgcgcccg gaatgctcgt cggggctcga gctccggggt
    24781 ccgccttccg tctccacggc aaacggacct gcccccccct gcccctctgc ccctgagcag
    24841 cgcggcgccg gggcggacgc gggataatgg cgaggtgaac agcgataccg ctgcgggtgc
    24901 tcggaagccc gtccggatgg tccgggtcct gcgggacgcc gccgaggacg acggctcgtt
    24961 cgacctcgcc ttttggcgcg aggtgggtgc cgagggacgg ctcgccgccg tctggcagat
    25021 ggtgggcgag gcccgggcat tccgggggga ggtgggccct gaaccccgac ttcagagatc
    25081 tgctgtccgc gttttccgcc gctgaggttc gcttcctcgt cgttggggcg cacgcggtct
    25141 cgtttcacgc cgttccccgc tacacgaagg acctcgacct ctgggtcgag ccgacgccgg
    25201 agaacgcagc ccgcgcctgg gacgccctcg cgcgcttcgg ggcaccgctc gaaggcgtcg
    25261 gcgtcgaaga cctctcgacg cctggtatcg tgatccagct gggtgtcgag ccgaaccgca
    25321 tcgacatcct gacccgtgtt gcgggactcg agttcggggc cgtctgggag aggcgcgtca
    25381 cctcgacgta cggaggtatc gccgtggcct tcccgtcgct ggacgacctc ctggcatcca
    25441 agcgcgcgtg cggccgcccg caggacctcc tcgacgccga ctggctcgag aaggcgaaga
    25501 agaacctcga acgctctcac gcccgcccac cttccagcgg cgagtagccg cggaccgggt
    25561 cgacgaccgc tgacacgtct tcgtccgttg gacttcggag acctccgaag acctccgggg
    25621 tcaggtccgt cttccgtttc aacggaaaac ggacctgacc ccctgccccg cggacctgtc
    25681 cccctgcccc gtgcctgcag gccgccgcgc gcgtctgcgc gagggcggcg gcgaatgcgt
    25741 ctacccttcc gcagcccggt acgcccgcca ggtccgttcg agctcggcga cgctctcccc
    25801 gagcgtcttc tcgaacgccg gcaggtcgac gggctttccg acactgcctc tccgaaggaa
    25861 cgcccggaaa cgcctgaagc gcgggagcct gccgtccgag aggtagcgga agaacgagat
    25921 cgtctcggcg cgcagggcct cgcccgccgg ccctctctcg aacagctccg gaccggcgcg
    25981 gaagagctcc gggacggacg gcggcggaag ggacgaagcg gggcggaccg ggagcggccg
    26041 ccgcgaccag gccgagcgcg acggagccgt gcccccctcg acgaaggcgt cggccagccc
    26101 ttcctcgacc cagttgggca cgccgtcccc gaggagctcg cgcgcgtaga ggtgccccac
    26161 ctcgtgcagg agcctctcga gcgtcgacgc ctcgccgtcg cctgcgaggg cgagggagac
    26221 gacgcgcgac cccgccgggc cgacggggcc gcaggcgacg gcggcgagct tccgctcggc
    26281 ctcggccgac tcgaagagga agacgtagga acccggcttc acggggacgc gcagcttcca
    26341 ccggtctccg agcgaggccg tagcggcctg gacggccttc tcgaggcgcg cgaggagtgc
    26401 gtcgtcccgc acgtcggtga cgagagtcct cctcgcgaac ggcacctcgc ggccgccctc
    26461 cctcatgagg gagcgggcgc gcgcgaggag cgttccggag acccactcct gcggcacgtt
    26521 cggtccgcct ctctcggaaa ggagggagac cggggcggcc tcccgcgccc agccgcgcca
    26581 ggcgccgtac ccgacgcgga cccagtcgtc cttcctctcg aggacctcga cgagggtctc
    26641 ctcgtcgagc atcgtgaggg cgggagagcc tggatcgggc tcgaggtgga gccgggcgcc
    26701 cgccggcagc gtgacgtgct cggcgggcgc ggggcgcgcg gcgaggaacg gcagcacgac
    26761 gaggaggacg gcgaggggac gcgatgaggc tctcacggga cgagcgagac cccaggcccc
    26821 gcggccgccg tcagctcgac ccttgccggg ttgaggcgac cgtgcagcca gacgatccgc
    26881 tgggacgaga gctctttctc gacgccccgg aacgccgaga gcagcgacga caggtccgcg
    26941 atgctggcgg cctccggcca gtgtgggcgc gcatcggccg gggccacgtc gggaggtggc
    27001 aggtccggcg cggtccctgc agacggcctc gagacgtccc agacgtggag cgggacgccg
    27061 agcctcgaga ggaacctccg ggtctgcggc gcctcgacga ggccctcctc gaccgcgccg
    27121 ctgccgagga ggaggacgac ggcccggcgc cggccccggg ccgccgccga aaggccggcg
    27181 accgcgacag cctcggcgac cctgggttcc tccgtgcgct tcgtgaacgc gagccggccg
    27241 acgacgcgga agaacgtccc gtcgctcggc gtgaagtcct cggaggcggc gaagaggtgc
    27301 acgacctcgg tcggagagtc gaccgtcttc gggatcgctc cctggaggcg atagcgtgtg
    27361 ccgcgtggca ggtcgatccg atccgcggcg gcgaccgcca cctgcgagcg gctgctttcg
    27421 ggcatgaggc ggcgcagatc gtcgagggcc tcgcccgcca cgacgaagac gacctccgac
    27481 ggcccctcct ccaccgcggc gaccctcagc ggcctctcgc ccgcccggaa ccacccttcg
    27541 agccgaccgt ccttcgggag ccgcgcgccg cggccgatgc ggatcgccac agccgtcagc
    27601 tcctcgagcg cctcgtcgag cttctcgcca ccgaggctcg ccaccgccgt cgccgcgagg
    27661 ccgtcctcga gctcaacgtc ggcgacgagg atgtacgtcg tccccgccga gtgttccgga
    27721 agcggaatgc gggacgggtc gggaacatcg agcggccggc cgtcgaagga gacgcggatc
    27781 gacttcggcc gggagccggt gacgctgtgc cagtggaggc gcgccgtccg gcccgtgccg
    27841 ctcgacccct tctcgacgac gaagctcgcc tccgcgccgg gccgcgggcg gttgatccac
    27901 tggcggacct ctccgacctg tcgcccctcg gcgtccttcg ccacggcgac gagctcccgc
    27961 gggacgatct cggggccgag gtcgagcttc gcgacccacg gccccgtcag ctccgagacg
    28021 aggtcgccgt cgacgtagag ctcgatccgc gccacgcccg gcgcggccga gagctcgatc
    28081 tcggccggcc cgtagaagag gccgaggaac agcgtcacga aagcgatcac gcgtctctct
    28141 ccgtgcttcg catcggcatc ccttgcctac ggcagcagct ctcgggcgac caggaagccc
    28201 ttctccatcg accggacgct ccggtggatc gccttctcga gcgccggcgc gtcgaacggc
    28261 cctctggata tgacgaagcc tttgaactcc aggaacgtct tcccgtctct ctccagatac
    28321 tcgaagaacc agcgcgcttc gcggcgcatg gcctcggccg ccggctcccg caagaagatc
    28381 tccggtccgg ccgcgaggat ctccggaatc gtcggcgccg gtttcggcgc gggcgcccgc
    28441 ttcggatggt aggcgggccg actctcggcc gacctgctgg cctggacgaa cctgtgggcg
    28501 agcccctctt cgatccaggc ggggaccttc tcccccagca ggtcgcgcac gtagaggtgc
    28561 ccgatctggt ggaggagccg cgccctttcc ccctctcgcc cctcgccgga gagggcgagc
    28621 gacacgaccc tggtgccagg cggcccggcg actccacagg cgacggagga gaacttccgc
    28681 tcggcctcgg ccgattcgaa aaggaagacg atcgatcccg gtggcgcggg ctcgctcttc
    28741 ccccagaact tctcgaacgc gaccctcgtt gcgacggcgg actcgacgag ccacttcagg
    28801 agggtctcgt cggagacgtc ggtgagaagg gtcctgccgt ccatcgggag ctcgcgcccg
    28861 ccattccgca ggagggcccg accgcgcgcg aggaggttcc gagggaccca tccccgcggg
    28921 gcgtccggct cgccgcgttc ctcgaggagc gatctcggag ctccctcccg cacccagccg
    28981 cgaaacgtgt cgtaccggac cagaatccag ccgtcctgcc tctcgaacac ctcgacgagc
    29041 gcctcctcgt cgaggaggac gagggagggt gaggtcacca cagggtcgcg atggagccgg
    29101 gcgcccgccg gcagcgtgac gtgctcggcg gccgcgggac gacccgagag gaggaggacg
    29161 cccgcgagga gcgcggcgag agatcgcgct gcggctctca cgggacgagc gagaccccct
    29221 tctccgccga cgcggtcagc tcgaccttcg ccgggttgag gcgccccttc agccagacga
    29281 tccgctggga cgagaggtcc ttctcgaggg accggaacac gcccaggagc gacgtcaggc
    29341 cggagacgcg caccgcctcc ggccagtggg agcgggcgtt gggaggcccc ggcggccgag
    29401 gggcggtctt cttcgcgctc ttcgccggag gtcgcgacac gtcccagacg tgaagcggga
    29461 cgccgagctt cgagaggcag cgccgggcac gctccgcggt gatcggcccc gcatccgtcg
    29521 cgccgctccc gaggaggaga acgacggccc tccgccggct cttctctgcc gccgagagac
    29581 cggagatcgc gacggcgtcg gcgacgcggg gctccccggg ccgatccccg ggggcgacct
    29641 tccggatcgc ggagaagaag tagtcgtcgc ccgcggagta gtcctcggac gagaagaagc
    29701 ggctcgcgac ctcttcgggg ccgcggacga cgcgcgggat cgtttcgacg aagcgatacc
    29761 gcgctccgac cggcaggttg gcctgcgcgc gcaccgcgag ccccgacctc tcgcgggtga
    29821 cctcttcaat gagcttgtgc aggtcgtcga gggctcggcc cgccacgacg aagacgacgt
    29881 tggagggccc ctcttcgacc gcggcgacct tcagtggttc tccgcccgcc cggaaccacc
    29941 cttcgaggcg gccgtccttg gggagcttcc cgccgcgagc gaggcggatc gggaccgccg
    30001 tcagctcctc gagcgcctcg tcgagcttct cgcctccgag gctcgcgacc gccgtcgccg
    30061 acaggccgtc ctcgaagtcg acgtcggcga cgaggatgtg cgtcgtcccc gccgagtgcg
    30121 ccggaagcgg gatgcgcgac ggatcgggaa cgtcgagcgg caggccgtcg aaggtgacgc
    30181 ggatcgactt cgcttccgag cgcgtcacgc tccgccagag gaggcgcgcc gtccggcccg
    30241 cgccgcccgg gtccttctcg acgacgaagc tcgcctccgc tccgggccgc gggcggttga
    30301 cccactggcg gacctcgccg acccgctgcc cctcggcgtc cttcgccacc gcgacgagct
    30361 cccgcgggac gatctcgggg ccgaggtcga gcttcgcgac ccacggcccc gtcagctccg
    30421 agacgaggtc gccgtcgacg tagagctcga tccgcgccac gcccggcgcg gccgagagct
    30481 cgatctcggc cggcccgtag aagaggccga ggaacagcgt cacgaaagcg atcacgcgtc
    30541 tctctccgtg cttcgcatcg gcatcccttg cctacggcag cagctctcgg gcgaccagga
    30601 agcccttctc catcgaccgg acgctccggt ggatcgcctt ctcgagcgcc ggcgcgtcga
    30661 acggccctct ggatatgacg aagcctttga actccaggaa cgtcttcccg tctctctcca
    30721 gatactcgaa gaaccagcgc gcttcgcggc gcatggcctc ggccgccggc tcccgcaaga
    30781 agatctccgg tccggccgcg aggatctccg gaatcgtcgg cgccggtttc ggcgcgggcg
    30841 cccgcttcgg atggtagagg gacctccagc ccttgcggtc ctcggccccc cggccggccg
    30901 cagcgagcgt gtgggcgagc ccttcctcga tccaggctgg gacgccctcc cccagccgct
    30961 cgcgcgccca gaggtgcccg atctggtgaa ggagccgcgc cctgtccccg gcccgcccct
    31021 cgccggagag ggcgagcgac gcgaccctga cgccgggcgg cccggcgact ccgcaggcga
    31081 cggaggagaa ctcccgctcg gcctcgggcg actcgaagag gatgacgatc gaccccggcg
    31141 gcgcaggccc gcccatcccc cagctgtatt cgaactcgac tctcgccgcg acgacggcct
    31201 cgaggagcca cttcaggagc acctcgtcgg agacgtcggt gaggagggtc cggccatcca
    31261 tcgcgatctc gcgcccgccg ttccgcagga gggcccggcc gcgcgcgacg agctcctgag
    31321 agaccaattc gcgtgggaga tgcgaatcgc cgcgctcttc gaggagcgat ctcggcgctc
    31381 cctcccgcac ccagccgcgg aacgtctcgt accggacgag gagccagccg tccttcctct
    31441 cgaacacctc gacgagcgcc tccgcgtcga gcagggcgag ggagggcgag gtcacatcgg
    31501 gctcgaggtg gagccgggca cccgccggca gcgtgacgtg ctccgcggcc gcgggacgcc
    31561 cggcgaggag cagcagcgcc gcgaggagcg cggagtgaga tcgcgaagcg gctctcacgg
    31621 gacgagcgag acccccttcc ccgccgacgc ggtcagctcg accttcgccg ggttgaggcg
    31681 ccccttcagc cagacgatcc gctgggacga gagctccctc cggagcgacc gaagcacgcc
    31741 caggagcgac ttcagtccgg agacgcgtac cgcttccggc cactgggggc gggcgtcggt
    31801 aagtcccggc ggtcgagggg cggtccccgg cgcgctcttc gccggagccc gcgacacgtc
    31861 ccagacgtga agcgggaccc cgagcttcga gaggcagcgc cgggctcgct ccggggtgat
    31921 cggccccgcc tccttcgcgc cgctcccgag gaggaggacg acgactcgcc ttcggctcct
    31981 ctccgccgcc gagaggcctg cgatcgcgac ggcgtcggcg acgtggggct ccccgggccg
    32041 attctcgagg acgaccctcc agatcgcgga gaggaagtcg tcgtcacccg cggagtagtc
    32101 ctcggaggcg ccgaagcggc tcgcgacctc ggcggggccg cggtcgacac gcgggatcgc
    32161 ctccacgaag cgatagcgcg tgtcggccgg gaggctggac tgctcgagca ccatttcgag
    32221 cccccccccg cggggggact cgtccagatg ccggcgcagg tcgtcgaggg cctcgcccgc
    32281 cacgacgaag acgacgtccg agggcccctc ctccaccgcg gcgactcgca gcggcttccc
    32341 gcccgctcgg aaccaccctt cgaggcggcc gtccttgggg agcttcccgc cgcggccgag
    32401 gcggatcggg accgccgtca gctcctcgag cgcctcgtcc agcttctcgc ctccgaggct
    32461 cgcgaccgcc gtcgccgaca ggccgtcctc gaagtcgacg tcggcgacga ggatgtgcgt
    32521 cgtccccgcc gagtgcgccg gaagcgggat gcgcgacgga tcgggaacgt cgagcggcag
    32581 gccgtcgaag gtgacgcgga tcgacttcgc ttccgagcgc gtcacgctcc gccagaggag
    32641 gcgcgccgtc cggcccgcgc cgcccgggtc cttctcgacg acgaagctcg cctccgctcc
    32701 gggccgcggg cggttgaccc actggcggac ctcgccgacc cgctgcccct cggcgtcctt
    32761 cgccaccgcg acgagctccc gcgggacgat ctcggggccg aggtcgagct tcgcgaccca
    32821 cggccccgtc agctccgaga cgaggtcgcc gtcgacgtag agctcgatcc gcgccacgcc
    32881 cggcgcggcc gagagctcga tctcggccgg cccgtagaaa aggccgagga acagcgtcac
    32941 gaaagcgatc acgcggactc ctccccgcga atcgaaagca atttgcgttc cccgggggcc
    33001 gtccccgaac ggggacggcg gcgagtgcag cgaacgcgac tacgcggact cttccggaac
    33061 cagccccagg aagaggcgaa gccatccctt tcggccacga cctcgtccgg gagagtggcc
    33121 gtgactcggg ccaggcgcat aggggcaggc ggcgccgctc agcacacctt ccaccacggc
    33181 gcggccagga tgtcgggagc gagccactcc gtcgtagtcc cggtgtggag gagcatcccg
    33241 gcgcgtgccg tttccccgta ttccgcgcgg aaggcgcgga ggttcgcgac gtccttcagc
    33301 cgggggcggc tcgtcgcctt gatctccacg ggcaggacct tcccgtccgc ctcgatgacg
    33361 aagtccacct cctcgcccgt ggtcgttcgc cagtagagga tctcggcgcg ctcggcgcgg
    33421 gagtcgcgcc aggcgagaag gtcgttcagg acgaggttct cgaggtgtga gcccgcgggc
    33481 tcggagagcc ccgccaggtg gagggcgagg cccacgtcac cccagtagag cctcggcgcc
    33541 ttgacgaggc gcttcgtccg gttcaccgcg taggcgggga tgcggacgag gatgtacgac
    33601 gtctccagga ggttcagcca gcgatggacg gtcggctgcg ggatcgcgac gtcgcgcgcg
    33661 acctccgtct gattgaggag ctgccctgtt cgcaggctcg cgaccctcat gagcctcctg
    33721 aagtccggca gggcggagat ggcggagagg gcctggagat cgcgttcgag gtaggtccgc
    33781 gtgtagccgt cgaaccagag cgcccggtct gccgcgctct tcatctcgag cgcgggcgtc
    33841 ggaaaaccgc cgcgccgggc gagcgccttc cagtcctccg ggccgggctc gccgccggag
    33901 agcacctcga gccaggtcga gtcgtcggcc gacaggagct cctcccacag cccgcagcgg
    33961 cccagcccga gctgctcgcg acgcgtcatc ggccataggg tcaggtggct ggcgcgtccc
    34021 gcgagggact cggagacgcc gcgcatgagg aggaggttcg ccgagccggt caggaggaac
    34081 cgaccggggc gccggtcccg gtcgatagtt ctcttgaccg cgaggaggag atccggctcc
    34141 cgctgcacct cgtccagcgt cacgggaacg gctcccccga cgagtgcctc ggggtcgcgg
    34201 cgcgcgaggt cgacgacgtc gaggtcgtcg agcgaaaggt accgccgctg acccggcgcg
    34261 aggacttccg ccagggtgct ctttcccgtc tgccgcgccc cggtaaccac cacggccggc
    34321 atcgtccgca gccggcgcgc gagtgccggc gcgacatggc ggggtcgcgg cgatctattc
    34381 atgccgtgaa tgatatccat tcacggcgtg gatggccaag ttcgggcgcg ggcgactcct
    34441 cctcgcccta ccccgtcgtc agcttccggt acttgatccg gtgcggcatg tcggcggcgg
    34501 cgccgaggcg tctggcggtc gagactggcg tagtcgcccg aatctctcgc tgggttacgc
    34561 ttcccccaac catggcgggc tgcctagcag acagcgatct cgaccagatc ctcgcccttc
    34621 agctgacgat cgcctgggcc ggcgagggcc tgtgtcagcc gccacgtctc gcgtggtggc
    34681 agacggatct cgtcgacggg cccgggggcg gtgatctctt ctcccggttg actccgaggc
    34741 tccggcactg gtcagcgctc gaagctgttc gcgagacggc ccggcgccac gacgcgagat
    34801 gccgccggga ggtcgcagag cccgagaagg tgcggacact cttcaacctc ggcttccgag
    34861 tcgaggagca gctcgggaca cggctctcga tcctgaagtc gtcagaagcc tcgccgagcc
    34921 aggttctccc cctcccgttg aacctggatt ccacgttctc cccggacgcg ctcacggcgt
    34981 ttctcgaatc cctgactcca cgcgtgaagc acacggcggt cctcggcgga agagagctct
    35041 ccggggcggt gctcaacgag tcactgatcc tcgcccgtca gcttgccgcc gcgttgatcc
    35101 ccctggccga tcactacccg atgcccttcg cgagggcttc cgcgtgatcg gcgaggagcg
    35161 gaggtttcac acccggctcc agaagacgac gctcgcggtc gtcgaggcga gggcgtactg
    35221 ggcgaaatcg tcttctggca tctcgaagga cgagcaggcg aggcgagcgt ttgaggagcg
    35281 atggttcggc gcacgaagcg ccgtccgcgt tcgcgtcctc ctggagagcc tgagagcccg
    35341 cttcgatacc ttcccagaag ccttcgcggt gcttcggcgc tggaggacga tcgaccccgt
    35401 gacacgggtc ctcgtctgcc actggcacct tcagctctcc gaccctctct atcgtgcctt
    35461 cgccgggacc ttcctgcccg cgcttcgtcg ctccggctcg tcggccacga gccggggcga
    35521 ggtccttcgc tggatggcgg ccatcgagcc cgcgcggtgg gggcccgcca gccgcatcca
    35581 gctcgcgagc aagctcctga ccgcagcgcg ggaggccggg ctgatcggac ccggccagga
    35641 tgcccgggcg atcctgctcc cttcggtccc ggacgtcgcc ctgggctacc tgctccatct
    35701 actgcggggt gtccggacgg agggctcgat caccgagaac ccctatgcgg cctcgatcgg
    35761 gctcgaagga gaggatctcg agaagagact tgcccgactc ccctggctga gctaccggaa
    35821 acagtccggg gtcgtggacg tcgaatgggc ggcaccggat ctgagctcct gggcggaagc
    35881 ggtcctttcc ctggaggccg catgagccct tcggagacgc cgcgcagtca gctggacgag
    35941 gccctcgccg cgctcgaacg cgacctcctc gcggagggcg gcccccggat cagcacgatg
    36001 cggaactaca acttcgccat cgtgccgtac ctgccggcgc aggagctcga tctaagaagg
    36061 aaggtccggg ccctcgccga caggctccgg gcctccggat tccgcgtcct cgccatctcg
    36121 ctccagaggc tcctcctcca gcggattcgg tccctcggcc ccgaggtcct gcctgccacc
    36181 acagctcgtg agaagcgact ctgggcgcga gacgcaggcc gggccctcgc tcatctcggc
    36241 gacgtcgtcg gcccgctcat cgaagggccg agcgggattg ccgccgacgt catccgcgag
    36301 atcgaagcgt tcgccgaggc tctccccacc ccggaagatc gaagcgtcgt cctcatcggt
    36361 cgcgccgggt ctctctaccc tttcttccgt tcctcggctc tcctgaagca catcgacgga
    36421 cacacgcgga acctcccggt cgtccttctc taccccggcg aacggcacgg gcgctcgtcg
    36481 ctctccttca tgggcgtcct cgcgcccgac ggggactacc gtccccgcat ctacccttag
    36541 tccggtttcc gaagcggagg ccccttgctc atccgagagc tcttcgccgc cgacgtcacg
    36601 cgcgacatcc cgcccgtcgt ctacttccac gagcaggctc cggagaggct cgcggacgag
    36661 gtccgcgagt acatcttcac cggcgggttt ccggagggcg acccgcgcgg aactcggggc
    36721 ggctcgggga tccacgagga ggtggtccgg ctcctgacgg gaatccgcac ggagctcgac
    36781 aagcccggcg gcccggccct cccggcctcc tggatctcgg gtttcttcgg ctccggcaag
    36841 tcgagcttcg cgaagctcct cggccttgcg ctcgacgggg ccttgctccc gggaggcgag
    36901 cctctctcgc aggctttcct ctcacgcgac gagtcgcccc tggcgcccga gctgcgaaag
    36961 gcctgggacg acctcctctc gaaggtctcg ccgatggccg tcgtcttcga catcggtagc
    37021 gtggcccgtg acggagagca ggtccactcc gcagcgaaac ggcagatcca gaaacgcctc
    37081 ggctattgcc ccgtgagcca cctcgtcgcc gactgggagg cgaagctcga ggccgacggg
    37141 ctctgggacg ccttcgtcct cgccgcggag aaggcgctgc gccgcccgtg ggacgtggcc
    37201 aaacgggagc agctcgcgcc ggaccacttc tcccacgccc tcagcgtcct cgatccggag
    37261 cgttaccccg aaccgacgac gtggatcgac agccacgccg gagaccgctc gagcgcgggg
    37321 agctccgtcg aggaggccgt ccgtgccatc gccgcgatgc tcgaccggcg cgagccgggc
    37381 aagacgatct tcttcgtgat cgacgaggtg agccagtacg tccaccacga cgagacccgg
    37441 atgctcaagc tccagacgtt cgtctcggac ctcgggcagc gcctcaaggg tcgcgcctgg
    37501 atcctcgcca ccggccagca gaagctcgag gaagaggccg cggcgaaccc cctgtcgaag
    37561 ctcaaggacc gcttcccgca gtctctgcgc gtccatctcg ccacgacgaa catccgcgac
    37621 gtcgtccaca aacgcctcct gaagaaagat ccggcgcgcg agcccggcct gcgcaagctc
    37681 ttccgcgagc accggacgga cctgaagctc tacgcctacg cctgcgaaga gatcaccgag
    37741 gaggagttcg tcgaggtcta ccccctgctt cccgggcagg tggacctcat ccttcgcctg
    37801 acgtcgagcc tccggagccg ttcctccaga gcccgcggag acgaccacgc gattcgaggc
    37861 ctcctgcagc tcctcgggga ggtcttccga agctgcgggc ttgcggacgc ggaagccggc
    37921 gcactcgtca cactcgacgc ggtcttcgag gtccagcagt cggccctcga cgtcgacgtc
    37981 cagacgacgc tcggccggat catggatcac gagatctgcc ggcgggaccc gctcgccctg
    38041 cgcgcggcga aggcggtcgc gctcctcgag ctgatccagg acgaaacacc caccacggca
    38101 gacctcgtca cacgctgtct ctaccagcgt ctcggcgaag ggaatcggac cgaggccgtg
    38161 acggcgtcgc tcgaggcgct ccgaggcgcc ggcctcctcg gttactccga gaggaccgga
    38221 tacaagatcc agagctccgc cggcggcgaa tggcaacggg aacgcgacga catcggcgtc
    38281 ccgggagagc tggtcagcgg gatcgtcgcc gagaagctcc gggagctcct cggagacgtc
    38341 gaccgcgggc gcctccaggg ccggccgatc tcgtggaccg tccacatctc cgacggtcgc
    38401 cacctgaagg agaagcctct cctcagctcc cgggacgaag ccacggtcgt cgtcgatctc
    38461 cgtttcctct ggaaccggga cgaccggctg tctgcccgtt ggattcccga gagcggcaag
    38521 gacggcctcc gcgaccgcct catctgggtc tcgtccgagc cggctctcgt cggggaggtc
    38581 gcccgggacc tcgcccggtc acggcagatg atcgagcgcc acaaagaccg tgaagcctcg
    38641 ctccctctcg cgaagaagcg gctcctcagc gaagagcgcg caggcctgaa cgacctcgag
    38701 acgaagctcg gctcggcctt gcgctccgcc ttcctcgaag gggctttcta tttccggggg
    38761 cgcgagatgc gcccgtccga cctcggctcc ggctttgcgg gcacgcttca gggcgccgcg
    38821 gagaaggtcc tccccgacct cttcccccac ttcctctcca tcgccgtcgc cgagagcgag
    38881 ctcctccagc tcctggaacc gaccctcgcc gggccttcga ccaagttcct cgagcagggc
    38941 ctcggcatcc tcgccctcga cgccgggaag tacgtcccgg cctgctcggg gcccgtgccg
    39001 acgcgcatcc gccagtacat cgagaaggag agcggcgtct ccggtgcgac cctcgtcgcc
    39061 agcttcggcg gcgttcccta cggctggccg gccgacgtcg tcaaagccgc ggtcgccggt
    39121 ctccttcgcg cccatgcgat ccggatacgc cccgaagacg gccccgagat ctcgtcgatc
    39181 aaggacccgg gcacgagaga cctcttccgc cgcgatcgag acttccggcg tgccgacctc
    39241 ttcccggccg gcgagagcga ggtcagcccg cgcgaccggg tcgccatctg ccagctcttc
    39301 gaggaccggc tcggcctcga cctcgatcgt gaggatgaag cgattgccga agccgtgttc
    39361 gcccacttcc ccctgagacg ggaacggctc ggagagcttc gcgcactcct cgtgggtttg
    39421 cccggccccc cgcccgtccc cgagccactc gtgcggctgg agagggctct gaccgagtgc
    39481 cagcgcaccc gacaggtcgc cgggatcgtg gccgccgtga agcgacacct cgacgacctc
    39541 cgggacggcc tgacgaccct cgaactcctt cgcgccgagc tgaacgacgc cgccatagag
    39601 gtcctgcgcg aagcggatcg ggtcgtctcc ggtgaggccc ggcagctctc cgacgcgggg
    39661 gctctcgaag aggtggccgc cgacgtcgaa gctcttcggg aacaccttcg acgggagcgc
    39721 ccctggcgcg aagcggtgca gctccggccg atcgtcgacc gtgtccgggc gagatacgtg
    39781 gacgctcgcc gaagccacct cgccgcccag gaggagaagg ccgaggcggc gcgagggcgg
    39841 atcgtggcgc gggacggctt ctcccgcctt tcgccagacg cggcgcaccg cgtccttcag
    39901 ccgatctcac gtgcgctcgt cgacacgacg gcggccgtac tcgctcctcc gctcgtggag
    39961 atcggcagcg gcgcgcttca gcgcctcgtc cgggcggagc aggaggcgaa cgagaggctc
    40021 gacgccgagc tggcccgcct cgattcgggc gccatcacgg tcgttccgct cgacgtccgt
    40081 ctctcaggcc gggaagtgcg aaacggcgag gagctcgagg cgctcttgag agagctgagg
    40141 gaacggatcg gggccgtgct cgaacgggga gagaaggtca ggctgctgtg acgaggcctt
    40201 ttctcacggc gcgtacaacg gcggcgcggc ctaaaatgcg ggcatgagca gcagccggaa
    40261 gaactccgag gatgctcgcg tggtgcgtga ggtccgggtc gaggaccttc tcgggcggct
    40321 caacgaggtc gaagagcgga acagtcccgg aaccctcttc gcgatcgggg agacgagcct
    40381 cctgcacggg acgccccgag ccgcgatcgt cggagcgcgg gacgcctcgg agctcggact
    40441 ggcgcgtgct cgaaagctcg cgcgacttct ggcgaggcgc ggagtcgtcg tcgtcagcgg
    40501 cctcgccgag ggcatcgaca ccgcggctca ctgggccgcc atccgcgccg cggggcggac
    40561 cgtcggcgtc ctcggcgcac ctctcgacgt cgtcttccgg caggcggatg tcgcgctccg
    40621 tacgctcatg gagcgagagc acctcgtcgt ttcccagttc ccggcgggcc acccggtcct
    40681 tcgctccaac ttccctcgcc gtaaccgcac gatggcgctc ctctcgcacg ccaccgtcat
    40741 catcgaggcg tccgattcga gcggttctct ctcccagggc tgggaggcgt tgaggctcgg
    40801 ccgacctctc ttcatcctga agtcggtcgc cgacgacccg cgactcacat ggccggccga
    40861 gatgacccgc tacggggcaa cggtcctctc cgaatccggc gtcgaagcgc tcttcgatgc
    40921 actgccgcct cccgaggagc ccggctggct cgatcgactt gctttctgag ctgacgttcg
    40981 gatcgctcct cgtctactcg ccgagaggct ccggggaggt ctccgtgagg tcccgaggcg
    41041 tcgtgcggtc gatcaagacc gacggtccgg ctcccggagg cgaagggatg atcgagtacg
    41101 ccgtccgtcg cctcgccgag gagctttcga agtgtgcccg ggccctcagc cctctgtttc
    41161 gcgaggacct gctcctcgtc cctgtcccat cgagtgcgcc cgtcctcccg ggtggcctct
    41221 gggtcccgaa gaggatctgt gaggcgctcg tcgccagcgg cttcggctcc cgggtcgcgc
    41281 ccatcctcac gaggacccac gccattccga agtccgcctt cgccggacag gggaagcgac
    41341 ccggcctcgg agtccacgaa gcgtctctcg ccgtcgcggg gaggtccttg ttcgcgggag
    41401 aaccggtgct cctcgtcgac gacgtcgtca cgaaagggac ggccctcctg gccgccgctc
    41461 gcgtggtctc cgccgcgagg cccgggagcg aggtctcggc tttcgcgctc gttcggacca
    41521 tgggtcttct tccggacgtc gagcgcatcc tcgacccctg cctcggcagg atttccctca
    41581 ccggcatgcc cccggaggcg cacagagagc catgaggcgc cgccgcgacg acccggagca
    41641 gaagaggctt cttacgcgcg aggagttcca ggcgcttctc tcggctcggg acaccgaggg
    41701 cctccggaag atgctctggg agctctacgt ccgaggtagc gaggacctcc ggcgccgaat
    41761 cgagacggtc gcgctgcccg tgggggagcg gccgaagaag gtggctccac ctcccgtcga
    41821 cggggaatgg catctgggcg aggtccggca gttcgtcgca ctggcgcggt ccggcgccta
    41881 cacgggcggc acccggcgcg tctcgccgtc cgagcgcacg cgctggcgcc tgccgttccg
    41941 cgccctcatc gacgattccg gccgcctcct cacgcaaggc gacgtcgcga acgggacgga
    42001 agcgatgagc gcgctcctcg acctcgcgct cgattgccgc gacacctact acttccggac
    42061 ggaagacccc gtctcggcgc tgcggatcgt cgcctccgat ctcgtcgacc tcctctggcg
    42121 cgcgaacctc gaggcccacg gcttcccggc gctcgtcgcc cgcgctccac gcgacctcct
    42181 ccggtgggag agcccctggg gctggacccg gatgggcacg cgcacgatcg cggcaaagga
    42241 gcgctccctc gccgacgtcc tcgccgcgct cctcccctcc cacgatgcct gggtcgcgat
    42301 ggccgaggcg tggctccagg tctttgcggc gactcacggc ggccgggccg ctccgcgctc
    42361 cgcgccgaac cggaacctgc cgagccagta cgtccccccc actccgtccg actggaagcg
    42421 ctggcacgag ctcctctacg agcgcttgca cggctccgag tcgcaccacc tcctcgagcg
    42481 gatcgcggct gtcccggagc gccccggcgt cgacctgtgg ctcctccgcg tccggctcgc
    42541 ccgggacgcc ggccgcgccg aggaggcccg ctgtattgca cgagccgccc tcggcgagtt
    42601 cccgggggct cgagagctcc aggccatcgc gagagaagtc ggcctccgct gaccccgcgt
    42661 ccccctcctc cccggcgggc cgcgcccgcc tctgcggcta cactctccgg cgaccccgcg
    42721 cagggaacct gttcagacgg aatgcggaag agcagacggc ggtacggagg gaagacggtg
    42781 gatcgaggtc ggcgctgaca tgaccctcac cccctccgtc cgccgccgcc tctcggagac
    42841 gatccgcgac ctgcgggcgc ggctgctcgc cgaccttcac gaggccgttg tcgccgaata
    42901 ccggctcaca ctccccatcg cgcggtcggg gctgagcgag gcggcggcca tcaagcgggg
    42961 gcggctcgag gcgttcctcg aggagaaggt ccgggcgggg cgcgcgaggg acgccgccgg
    43021 gaaggaggcg atccggaagc gcgcgctcga aggggcggtg aaagaggccg cgtcgacgct
    43081 cctcaaccgg ctcgtcttcg tccgccggct cgaagcgctc ggcctctccc ggccgctcgt
    43141 cctgaccggc ggctggcgaa gccagggctt tcgggagctc atcagcttcg ctccgggcct
    43201 cacgatcgac tcgggcgacc cggccgaagg gtacccgttc ctcctgaagc tcctcttcga
    43261 cgagctggcg ctcgacctgc cgggcctctt cggcgaggtg ggcgtctcgg cgctcgtccc
    43321 cgttccgctt cccacgctcc gggccgtcgt cgaagcgctc gacgcgcccg acctcgcgcc
    43381 cgcctgggac gacgacacct ccctcggctg ggtcttccag tactggaacg accccgagcg
    43441 cgaagccctc gacgccaagg tcctcaccgg cgggaagatc gccccgcacg agatcgccag
    43501 caagacccag atgttcaccg agcgctacat ggtggagtgg ctcctccaga acagcctcgg
    43561 cgacctctgg ctcgagatct gccggaagaa tggctggaag gccgacgcgg aagaggtctt
    43621 ccccgcgctc gaggagcgcc gcgccgcctg gcgcacggcc cgtgcgaggg acgaggtgcc
    43681 gccgacggag ccgatggccg tctccgaggg tctcgaggag cggtggaagt ggtgggtgag
    43741 gaagcccgcc ccggaggagg tcccggccgg gacgccccct tccatccgcg acgtgaagct
    43801 cctcgacccg gcctgcggct cggggcactt cctgctctcg gctttcgaca acctcgcggc
    43861 gctctaccag gaagaggcgc ggcatcgggg cgttgtgtgg agcgaccggc agatcgccga
    43921 ggagatcctc gagcggaacc tcttcggcgt cgacatcgac ccgcgggccg tccagatcgc
    43981 cgccgccggc ctctacctga aggcccgggc gctcgaacgg aacgcgcgcc cccgcgcgct
    44041 gaacctcgtc gcccccgccc tcggcctcgc gagcctcccg cccggcgacc ccgccctcgc
    44101 ggcgctccgc gacgagctga ggacggaggc cggcatcccg cacgacctga ccgacgccgt
    44161 cctgacggcg ctctccggtg tcgaccacct cgggacgctc ctgaaggtgg acgaggcggt
    44221 ggaggaggcg atccggaagg cagcgggggc cccgaaggtg gaggccgtcc agaagaagct
    44281 cttcgggggc gacccgcccg cgcaggcgaa gctcgactac gacgccgcgc gcgcctcgat
    44341 cctcgagaag atagagggct tcctcgggag gcatcgcggg gaggatgacc tcggcctccg
    44401 cctcgacggc gaagcgctcg ccgcgggcct ccggttcctc cggatcgtga agagcgggac
    44461 gtacgacgtc gtcgtgggca acccgccgta ccagggcgcg agccggatgg ccgaggccgg
    44521 atacctcggc cgcacctacc cgcgcgggaa ggcggacctc tacgccgcct tcctcgagcg
    44581 ggcgctcgac ctcgcgcggc ccggcggcct ctcggccctc gtgacgatgc gcggctggat
    44641 gttcaccgcg cagtaccagg ccctccgcga gcaccttctg acgacgtacg acctgcgcgt
    44701 tctcggcgac gtcgaccgcg gggccttcga cgaggtgccc gacgaggtcg tcgccgccgc
    44761 gatgagcgtc ttccggaagg cgccgccacc gggcctccca agcgtcgccc tccagcccac
    44821 cgctctgacg gataagtcgc gcgaccgcga gcggaccgag cgaaagcgcg cggcgctcct
    44881 cgcgcaggtg gggcggtacg agttcgaggt cgcgcggctg aaggtcgtgg ccggggagcc
    44941 gctgctctac tggtggagcg aggagttcct gaaggagtac gcggaggcgc cgaagctggg
    45001 ggacgtggcg ccggcgcggc aggggctggc cacggccgac aacgcccgtt tcctgaggcg
    45061 gccgtgggaa tgccggatga agcccgggcc gatcagtagg gatgtcgccg acgcggaggc
    45121 cgtttcattc ggaccgtggg cgccattcgt gaagggcggc gaagggcggc tctggctcga
    45181 accactctcc gatctcgttc tctgggaccg gcaggggctg gagctcaaga ccttcacgga
    45241 gttctggtac ggaagcgcga gcaggaacat caagaacgag gacgtctact tccgacgtgg
    45301 ggtggcgttc tcggcgatcg ggaatgcctt cggtgctcgt gcgcaccgag cccacagcat
    45361 catcggccat atgggcgcgt cctcattccc tgaagacatg ccgatggcgg tctgcctcat
    45421 gaactcgtgg acgtcgcgct ttgtcctggc atcgctcaac ccaggtctcc atttcgaagt
    45481 cggcgacgtc aatcgcctcc ccatcttccc cgtcgagagc tccgccgaga tcttcgagac
    45541 gctcgacgcc gctttcaccg agcacgaggc cgcccgcgag acctccgtcg agttcaagcg
    45601 gccggggccg tccgcatgga cctcagccca agcctgggcg caggaggccg tcgatcgcac
    45661 caagggttcc cctctccctc cgtacgagcc cgtctatgag gccgcatccg ccttccagca
    45721 cgtctccttc gccttcggcg tggcgatggg gcggttcggg gcgaacggcg aggggattct
    45781 cgaagtggct ccggcggacg cccttcccgg cggcatcctc ttcctctccg aggcgaccgg
    45841 gcgcgacagc ctcgcgcatt cggcctgcgg gccgctccac gccgcgtggg ccgatcacgg
    45901 tgcggcggtc ggcgaagggg acgatcttcg ctcgtggctc cggaagtcgt tcttcggctt
    45961 tcaccggaag ggctacgagg accggcccat ctacttcccg ctctcctcgg cccgaaagac
    46021 gttcgtggcg tgggtgtcga tccaccggtt cggccccgac acgctgaagg ccgtcctggc
    46081 ggaccacgtc cagcccgagc tgcggacgct cgaaggcgag ctggaagacc tcaaggccgc
    46141 tcggcaggcg tccgaccggc gggagcgggg gcgggcggag aagcgttacg ccgagatcca
    46201 gaagcagctc gccgagctcc tcgacttcgt aggcgcgcta cggcaggtcg ccgagaaggg
    46261 gccgccgccc gtcgacgcgt cgacgccacc gcgggaagcg gacgctccgt tcgagatgga
    46321 cctggacgat ggggtcatgg tgaacgccgc tgcgctctgg ccgctcctcg cgcctcagtg
    46381 ggggaagccc gcgcagtggt ggaaggagct ggcgaaggcg gaagggaaga aggactacga
    46441 ctgggcgcac cttgcggccc gttacttccc gtcgcgggtc gagaagaagt gcaaggacga
    46501 cccgagcctc gccgtcgcgc acggttcctt ctggcggctg catcccgcga gggcctacgc
    46561 gtgggagctg aggctgacgg acgagatcgg ccccgacttc acgatcgacg aagagggctc
    46621 ggacacggcg cgggaacggt tcctcgccga ggagccggag cgggcggccg acctccggaa
    46681 gaaggaacag gaccggaagc ggcggaagct ccttcggacg ggcggcagct ccgaggagct
    46741 gttcgacgaa gaggccgagg cggaggacgc gcccgccgag acggaggaag cggatgcctg
    46801 agcgcccggt ctcggccgcg ctggaagcgg cggttcgcga ggaggtgcgg ctccacggga
    46861 tcgtcttctg gctggaccgg gaagggacgt actcggcgtt cgtcgacgac ctcgtcgccc
    46921 ggcgggccgc ggggacgttc ccggttccgg tcgtggcgtt ccgcgggagc tatctcgaga
    46981 cgctcctcgc cctcgagccg tatggaagcg gcctcgatcc ggagcctctc ctcgtccacc
    47041 tgccgggttt cacggaggag acggtccggg aaacgccgct cttcgagctc tacgaaacgg
    47101 gggtgcggtt ccggaaggcg ctcgacacgc tcgtaaggga ggccgcggcg ggaaaggtgg
    47161 ctccgaaggc ggtggaggga atcctcgccg gaccgggact ctcgctcgag gcggccgacg
    47221 ccgcgctggc ccaggcgatg gaggcggcga cgggcggccc gggagccttc ctttcggcgc
    47281 tgaaaccgga ggtgcttctc gagggcctcg tcaccggcga ggccgacctc ctcgcgcgca
    47341 tctcggacgc aaccggcgtc gcagcgctcc ttcaggcgct cgagcggcag acggggatgg
    47401 acgaggcgtg gcgaggcttc gccggggcgc ccgcacccgg agcggagggg cgaggggccg
    47461 tggagcaggc cgcgtccgcg gccgtctcgt gggtcctcgc catggagtac gtccacgacc
    47521 tgaagcgcgc gccggagctt cccgccctga ggccgctcgc aaaggccggc aagccggtcg
    47581 tcgcgacctg cggccgcctc gcggcgcacc tgcgctcgcg ccagccggag ctctacgagc
    47641 gcctcgccga cgagacggag ggggtcctcg ccggggagct ttcgacgatg agggccgagg
    47701 acctcgggcg gatcgacacc ttccggtcgg aggagcggac gatcctcgag gccgccatcg
    47761 aggccgtgcg ggccggggcg cacgcgcggg cactggagtg ggccgaggcg cggcgcgtga
    47821 ccgggtcgtt ctgggcgcag cgggagccga cgcacgcctg ggagtggagc ctcgtgcgtg
    47881 aggccgccac gctggggcgc ctcctcgccg agatgcccgt accgctcgcc ggtgcgcggg
    47941 acgtcgccga cgcgctggag cgctacacgc gcgaggcgca tcgcgtggac caggcgcatc
    48001 ggaggctgga gcagcgccgc gtggcgctcc tcgagccgcg tctcccgcac ttcggggcgc
    48061 ttcagggggc tctcgcgctc ctccggcggg gctaccggga gtggtccgac cggctggcac
    48121 gcgacttcac cgcgctctgc cggatgtacg gcttcctgcc gccgtcgtcg atccggcagc
    48181 gatccttctg ggacgaggtc gcccacccgc tcgcctccgc cctcgggacg acggccgtcc
    48241 tcctcgtcga cgccctccgc ttcgagatgg cggccgagct ggccgaggag ctgcggaccg
    48301 aacggcgcga cggtgcaggt gtctcgctct cggggcgcct cgcggagctt ccctctctga
    48361 cggcggtcgg gatgaacgcg ctcgctccgg tggcgacgga cgggaggctt cggccgatcg
    48421 tgacgaacgg gtcgttcgaa gggttccgga cgggcgaggc cacggtgcga aagccggccg
    48481 accgggcaaa ggccatgggg gcccgctcca cgggcgcccc ggcgctccgc ctcgacctga
    48541 aggagctcgt cgagctggac gtttcggagc tgactcgaaa gctccgcgga gcccggctcg
    48601 tcgtcgtgac gagccgggag atcgacgacg cgggcgaggc gaacgtcggc ctcgcgacgt
    48661 tcgagcagac gctccggcag ctccggtcgg cgatcttcct cctcgaaggg gccggggcaa
    48721 aacggatcgt cgtgaccgcc gaccacggct tccttcttct cgacgagacg acggcagtgc
    48781 ggccctacgg aagccgtcgc gacccggagc gccggcacgt cttcgcgacc gaaagacggc
    48841 aggagacggg catggtcgcc gtgccgctcg ccgagctcgg gtacgacggt gtcgacggga
    48901 acctcctctt cctcgaggac acgtccgtct tcgcgacggg cgtcaccggg gccacgttcg
    48961 tccacggcgg caacagcctt cccgagcggg tgatcccggt cctcgtcctc cgcgaggccc
    49021 gcgccacggc gacgggcgag gcggaatggc ggatcgaagc ggaggcctcg gccgggctgc
    49081 tcgggttctc ctgcctgaag gtgcggatcg cccccgcccc cgggagcctc gcgttcgcgg
    49141 agcgctccga ggtgacgctc gccctccgtg cggcagggcg gaccgacgtc gacgtcctcc
    49201 tccgcgacgc gcgcgggaac ggcaccgttc ggggcgggcg gctcgagctg cgctccgggt
    49261 cggagtggac cgagctcttc ttctcgctcg agggcccggt ggacgagaag gccagggtgg
    49321 aggtcttcca ccccgacggc gtcgtccgcg ccgcgccggt gagcccggaa gcctggttcg
    49381 gcgtgacggg acgaaagccg agcggcgcct ctccgatatc ccccgcgcgc gccgcgtccg
    49441 ccgccgcctg ggagagcgcg ctcgaggacg agggggcccg gaggatcttc gtccacctcg
    49501 cgcagcacgg ctcgatcacg gaggtcgagg cgacccgtct cctcggctcg gcccgcgcct
    49561 tccgggcgtt ttcggctcgc ttcgacgccc tgagggcccg agtgccgttc gagacgcgcg
    49621 tggagacgac gcccgagggg aaacggtacg tgcgggaggg ggagaggtag ggacatgccg
    49681 agccgtcgcg acgtcgacca tgtcttcgag cggcttcgct cgggtctcgt gccggagcgc
    49741 gggctcgagg agttcgccgt cggaatcgag cggccgcggg gcgagatccg gcggctcctc
    49801 gacctcgccg gccggggcga gggcgtcttc aagttcctcc gtggcggtta tggctgcggg
    49861 aagacgttcc tggcccgact cgccgtcctc gacgcgcagg cagccgggtt cgcgacgagc
    49921 ttcgtcgtcg tctccgacaa cgacctccac tttcaccgct tcgacgagct gtatcggcgg
    49981 gtggtccagg agctcggaac ctcggcctgc ccgcgcgggg cgctggccga cgtcgtcgac
    50041 cggtggatcg cgcgagtcga ggaggcgctc gtcgccggtc tggaagatgc ggacggagcg
    50101 ggtttcgacg aggcggtggc ccgaaggctc gcggaggagg tgacctcccg gaccgcgggg
    50161 aaggctcccg aggacctcgc ccgcgtcctc cggacgatct tcgcgctgaa gcaggcggga
    50221 gagctgtcgg acgcgggcgc gctcctgtcg tggctttccg gaagcgagaa cgtcgcggcc
    50281 ggggtgaagc gagcggcggg gatcaagggc gacatcggca gccgggaggc gatggactac
    50341 ctccgcgggg tcctcgagat cgtcaaggcg gccgggtaca aggggctcgt catcgtcatc
    50401 gacgaggcgg agaccattct ccggatgcgg caggacgttc gcgggaagtc gctgaacggg
    50461 atccggcaga tcatcgacgc ggcagcgcag ttcccggggc tcttttggct cttcacgggg
    50521 acgcccgagc tcttcgacac gcggcggggc gtcgccggcc tcgcgcccct ccacgagagg
    50581 atccagttcc tgcgccacgg cgagagcgcg agccttcggc agccgcagct cgcgctgacg
    50641 ccgttcgacg aaacgaggct ccgcgaggtc gcgctccgcc tccgggagct ctatccggcg
    50701 tcggacccgc accgggtcga agcgaaggtg acgcgcacgg tgatcgatgc gctcgtcgcc
    50761 aaagtgacgg agggattccg cggcgacgtc ggcgtcgtcc ctcgccagtt cctcaggcgg
    50821 ctcgtcgacc tgatggacct cgtggaggag gaccccacct tcgagccggg tcgggaagga
    50881 gcgccgcgga ccgaagacct gaacgagccc gagcggcggg tcgcgaaggg agagcccccc
    50941 ttcgacccgg aaccggagga cgaagagggg tattcgcccg cgctcgtcga ggtctgaggg
    51001 aggctcgtga cggtccttcg ccggttcccg ccgcggcttc gggaggcgat cgggcagcgg
    51061 ctcgggtggc cggccctccg gcccgtgcag gaacaggcgg ccgaggccct cctcgatggc
    51121 cgcaacgccg tcgtgctggc cccgacggcg ggcgggaaga cggaagctgc cctgtttccc
    51181 atcctcgccg gcctcgtcga gaacgagccg aacggagtcg gtgcgctcta cgtctcgccc
    51241 ctccgcgcgc ttctgaacaa ccaggaggag cgtttcgggc tctacaccga gatggtgggc
    51301 ctcgagagct tcgtgtggca cggagaccga acggcgagcc ggaagcggcg cttcctgctg
    51361 gaaccggcgg cgatcctgat gacgactccg gagtcgctgg aggcgatgct cctctcgcag
    51421 cgggtccccg tccgcaggct ctttgccgac ctccgggccg tgatcgtcga cgaggtccac
    51481 agcttcgcgg ggacggaccg tggggctcac ctgatgtcgg tcctcgagcg aatcgccccg
    51541 atcagcgggc acgacgtgca gcgcgtgggg ctgagcgcga cggtcgggaa tcccgaagcg
    51601 atcctccgct ggctcgccgg ttcgtcacga cgggaggggc aggtggtcca gcccgctggt
    51661 ggccggagcg cgcgggagct tctcgttctc caccgtgaag ctccgggcga cctcgcccgc
    51721 gaggcgtccc ggctcggccg ggaccggaag gtcctcgtct tctgcgccag ccgggccctg
    51781 acggagaagg tcgcggggag gctccgggat ctcgggacgg acgtcttcgt tcaccacagc
    51841 tccgtctctc tggaggagcg gcaggcggcg gaggagcggt tccggtcgag cggcccggcc
    51901 tgcatcgtcg cgacgtcgac gctcgagctg gggatcgacg tgggcgacct cgaccgggtc
    51961 ctccaggtcg acgcgccgag cacggtctcg tcgttccttc agcggatggg ccgaacggga
    52021 cgccgcgacg ggcagacgtc gaacacgacc ttcctctgcc aggaagcgga gagcgtcctc
    52081 ctcgccgtcg cgctcgtcga gctcgcgcgg gaaggctggg tcgagccggt cccgccgcag
    52141 acgcgctgct ggccggtcct cgtgcaccag gccctcgcca tcgcgctcca gcacggaggc
    52201 atccagccgg gcgaggtccg cgcgctcccg gaacgcgtac cggatttctc ggggatctcc
    52261 agcccggaga tcggcgagct gaccgagcac ctggtcgccg aggggtacct gtcgagcgcc
    52321 ggcggccgcc tcctcttcgg agagcgcgcg gagcgggaac tgggacgtcg gaacttcctc
    52381 gagctctacg cggtcttctc gactcccgcc ctctaccacg tggagacgcg ggcgcggcag
    52441 gagctgggga cgctggagca gagcttcgtc gagcggctcg aggacggaaa gagctgcttc
    52501 gttctcgcgg gccgcccctg ggtggtcgtc tccgtcgccc acaaggagcg tcgcgtggag
    52561 gtcgagcccg cgcctcgcgg acggacgccg acgtggggcg ggttcctgcc ccagatcctg
    52621 agccgggagg tctgcgcgag gatccggcgg ctcctgaccg aggaggccga gccggagtat
    52681 ctcgctccgg ccgcccgccg cgcgctcgct ctcctccggg aggatcgggg cacggtgctc
    52741 gcgcagtccg ggatgccgct cctcccgggg tccgaagccg ttcgcgccct gacgtatgcc
    52801 ggaggccggg tcaactacac gctgaaggct gttctcgagt cgctgcattt ggtccgggtc
    52861 gtcgccgaca acttcgagat ccgggtcgag gggagcaccg ccacgcgaga ggccgtcgag
    52921 tcgaccctcc gggcgatcgc cgttcccggg tacttcgacg acgccgagcg ggtcgagagg
    52981 ctccgggcgg ggctcccgcc gttccgcctg agcaagttcc agccgctcct gcccgcgcgg
    53041 ttcgcgcgag aggtcatcgg caggtacttc ctcgacttcg aggggacggc acagttcgcg
    53101 agggacgtcg tcgggagttg acggagagtg ggcctcgctt ccgaggccgg gcctaccgac
    53161 ctgctgggcg tcaggcgggg gtcgcgccga tccggcgacg aagcccgttc aggtgcgaag
    53221 gcggaatcgc tccctcgtgt tgcaggcggc ccacgacgat cccgggggcg attccctgct
    53281 cctcggcgaa gcgccggagc gaggcctcgt ctcgtctcca cgaccgtagg aagccagcca
    53341 gggaccgcgt cgggagaagg gtctccgccg cgaatcggtc cgcttcttcc tcctctcggg
    53401 aggtcgaggt cccgtcctcc acgaagaagt ctttcttgcc gtgtcggagg aggtgtcccg
    53461 cctcgtggaa gaaggtgaac cagaagtgat cctccgtccg atagcggaaa ctgagctgga
    53521 cgagcgcacg cgtgggcgag agccatcggg tcgccccgaa gacccgggcc ttcggcagct
    53581 cgggctccac gacgagaacg acgccgaccg aggcgcaatg cgcaacggcc tccggcaggg
    53641 cgcagtcgaa cggccggagc gtcagcgagc ggagcgaacg gagaacgtcc gtgaaaccag
    53701 cggcgctcca gggcttcgtt tccacgtcgc gagcgaggag ctctccacgc cggagccaag
    53761 cgaacaggga cttggggtcc ccctcgaagg cccttgcctt ccggtactgc gccgcgacgt
    53821 ccccccagac gacttcccag gcgtcgacgc ttgcgacacc gaagaagacg aggagggcgt
    53881 tcgctctttc cgcggggttc ttgccggcag gaagccatcc ccgcttctcc atcgcgcgga
    53941 ccgggaaccc tctcgcccag tcctcgcctt tcgaggactt ctcgtccgcc gcgacgcggg
    54001 ccttgaactc ctgatagcgg gcttcccggt tcagccagaa ggcggccggg acgctcagga
    54061 ccctctcgag ctgcagcgcg gtctcgggcg tgatggcgga cttgccctgg acgatctcgt
    54121 tgatcgcctt gagagtcttc cccgtccgct cggcgagatc cgcctgagtg atgtcccagg
    54181 cctcgatcgt ctcccggaga gtctctcccg gaggcgtcac gaaatcgggc tggtaggggc
    54241 ggtaggccgt ctcagccatg ggtgtcctcg attcccagga tcaggatcgc cgtgacgcgg
    54301 ccccagtcga ggccgccgtc ggttctcaaa ggtggggggt ccgcgagcgg tcgaaacagc
    54361 aaacggtaag ggtggtcgag gtcgacggaa agaatgccag ccagatcgcc tacgagctca
    54421 tgacatcgag gccccggcag cttcgcaacg tcggcgagag tcgaagccgc gtcgagctcg
    54481 tccagtcgcc gacgaatcag ctgcgctcga cggtcaccgt gcgctcggac gagatcccgg
    54541 aaggactcgg cttgcttctg gagctttcgg ctcttgaaat ggagctccac ggcggcgagt
    54601 cgaatgatac acccatcggg tgcatcaaag ccatccaaca tcctcctccg ccggacacgg
    54661 ctccagtccg acggttggcc ccagcgccgg aagtggcgcc ccgcccccta ccccgtcgtc
    54721 agcttccggt acttgatccg gtgcggcatg tcggcggcgg cgccgaggcg cttgtgccgg
    54781 tcggcctcgt actcctggta gttcccctcg taccagaacg tcttcgagtc tccctcgaag
    54841 gcgagcatgt gggtcgcgac gcggtcgagg aaccagcggt cgtgcgagat gacgacggcg
    54901 cagccggcga aggagaggag cgcctcctcg agcgcccgga gcgtgtcgac gtcgaggtcg
    54961 ttcgtcggct cgtcgaggag gaggaggttg ccggccttct tcacggtgcg cgcgaggtgg
    55021 agacggttca tctcgccgcc cgagaggtcg ccgatccgct tctgctggtc ggcccccttg
    55081 aagttgaaca tcgagcacca ggcgcggctc gacatctcgc ggcggccgac catgatcttc
    55141 tcctcgccgc cggagacctc ctcccacacg cacttcgtcg ggtcggggcg gtcgcggttc
    55201 tggtcgacgt acgaggggaa gaccgtctcg cccaccgtga ggacgccgcc gtcgggcttc
    55261 tcgaggccca tgatcatctt gaagagcgtc gtcttgccgg cgccgttcgg gccgatgacg
    55321 ccgacgatgc cccccttcgg gaggtcgaac gtcaggtcgt cgaagaggag cttctcgccg
    55381 tacgccttct tcagcccttc cgcgcggacg acgacgtcgc cgaggcgcgg ccccgccggg
    55441 atgtagatct cgttcgtctc gccgcggctc tcggccccgc tctcggagag gagctgctcg
    55501 taggcctgca ggcgcgcctt ccccttcgcg tggcgggcgc gcggggccat tcgcacccac
    55561 tccagctcgc gctcgagcgt cttctgcttg gccgtctcgg ccttctcctc ctgctcgagc
    55621 cgcgtcttct tctgctcgag ccaggaggtg tagttcccct tccaggggat gccggcgccg
    55681 cggtccagct cgaggatcca ctgcgcgacg ttgtcgagga agtagcggtc gtgcgtgacg
    55741 gcgacgacgg tgccggggta ctcggcgagg tgctgctcga gccacgcgac cgactcggcg
    55801 tcgaggtggt tcgtcggctc gtcgaggagg aggaggtcgg gcttctcgag gaggacgcgg
    55861 cagagcgcga cgcggcgccg ctcgccgccg gagatcttcg tgacgtcggc gtcggggggc
    55921 gggcagcgga gggcgtccat cgccgtctcg agcgtccggt cgaggtccca gccgttcgcg
    55981 gcctcgatgg cgtcctgcac cttcccgtac tccgccatca ccttgtccat ctcggcgtcg
    56041 ggaaggctct cgcccatctt ctcgcccatc tcgtcgaagc gcgcgaggag cttcttcacc
    56101 ggctcgacgg ccgcctcgac gttctggagg acggtcttcg aagggtcgag ccacgggtcc
    56161 tgctggagga acccgacgcg cgtcccctcg gccgggaacg cctctccgtt gaactcccgg
    56221 tcgactcccg ccatgatctt caggagcgac gacttccccg cgccgttcag gccgaggacg
    56281 ccgatcttgg cgcccgggaa gaacgacagg tagatcccct tcaggatctc gcgctgcggg
    56341 gggacgacct tgcggaggtt gaccatcgtg tagatgtact gctgcgccat ggcgggcgga
    56401 atgtagcagg ggtgcggcga aggcgctggc cgtgcatcgc cggggcgccc ggcctctccc
    56461 cctcccgtaa actccccggg cggcccgtcc cgggccggcc gcggagacct caggccatgg
    56521 agaccgcatg agacgagccc ttcccctcct cgccctcctt ctcgccgcgc ccctcgccgg
    56581 gcaggagaac gcgattcccc gcgtcccgat ctcccctcgc ggcgccgaag agatcgccgt
    56641 cgggaacggg acgatgcgcg tcgagtacgg ccggccgttc gcgaagaagc ggaaggtctt
    56701 cggcggcctc gtgccgtggg ggaaggtctg gcggaccgga gcgaacgccg cgacgacgct
    56761 tcgcctcgac gtcgacgtca cgctcggcac cgcctccctg ccgaagggga cctacacgct
    56821 ctacacgctc cccggcccga aggaatggca gctcatcgtg aaccgccaga ccgggcagtg
    56881 ggggacggag tacgacccga agcaggacgt cgtccggatc ccgatgaagg tcctctcccg
    56941 gcacgaccac ctcgaggcga tgacgatcga cttcctcccc gaggacgcct tccgcggctc
    57001 cctccggatc tcgtgggaga agaagatgct cgtcgtcccg tacgcgttcc gaaggtgacg
    57061 ggcgcccttt ccgcgcgccc ggaggagaca gatcgatgac ctcgaagagc ccccgcgcga
    57121 tcctcgtcgc cgcgatcgcg gccgccctcg ctctgccgct cgccgcagcc cccgcgaagc
    57181 gcgccgccac gatcgcggac ctccacgcgg tccagggggt cggcgagccg gtgatcgccc
    57241 ccgacggcag gagcatcgcg ttcaccgtga cgacgaccga cctgccagcc gtcaagcgct
    57301 ggacgaacct ctggcgcgtc gacgccgacg ggaagaacgc ccgggcgctg accgtcctcg
    57361 acaagcgcga cgcgagcccc gacttctccc ccgacggcgc gacgctcgcg ttcctctcga
    57421 cccgctccgg cgcgccgcag gtctggttcc tcccgacggc ggggggcgag cccgagaaga
    57481 agaccgacct tcccggcggc gtcggagcgt tccgcttctc gcccgacggg aagaggctct
    57541 tcctcctcgc cgacgtctgg cccgcctgcg gcgccgacct cgactgcaac cggaagaagg
    57601 acgaggcgat ggagaagggg aagatgaagg cggtcgtcgc cgactccctc ttcgtccgcc
    57661 gctgggactc ctgggaggac ggcaagcgga ctcacatcct cctcatggac ctgtccgacc
    57721 cgaaggcgcc cctcgtcgac ctgacccccg gcgacttcga ctcccccgcc ttctccgccg
    57781 gcggcggcgc cgacttcgac gtctcccccg acggaaagga gctcgccttt acgtcgcacc
    57841 gcgacccgtt cccggccgcc tcgacgaacg tcgacgtcta cgtcgtcccg gtcgacggga
    57901 cggaggagca gcggaaggcc ccgaagaacc tgaccgcgaa gaaccccgcc gccgacggcg
    57961 cgccgcgcta ctcgcccgac gggaagcacc tcgcgttccg gatgcagcgg acccccggct
    58021 acgagtcgga ccgcttcctc ctcgcgctcc acgaccgcgc cgccgggacg agccgcgtcc
    58081 tgacggaggc gttcgactcc tgggtcggcg actaccgctt ctcgcccgac gggaagaagg
    58141 tcctcttcac ggccgacgtg cggggccgga cgccgctcca cgagctcgac ctcgcctcgg
    58201 ggaagatcga ggtcgtcagc tcgaccggtt tcctcgacac cttccaggtc gcgcccgacg
    58261 ggacgtgggc cgtcctcgcg cggcggcaga tgcacctccc gaccgagctc taccggctca
    58321 cgctcgccgg gaaggagcgg ggcaaggtga cgccgctgac gagccggaac gagaagctcc
    58381 tctccgaggt cgccctcgcc ccgatggagg aggtcttcgt cgacagcccg accgggaaga
    58441 aggtgcaggt cctcctcgtg aagccgcacg acttcgaccc ggcgaagaag tacccggtca
    58501 tcgtgaacgt ccacggcggc ccgcagatgc agtgggccga cgccttccgc ctcgacgcgc
    58561 aggtctaccc gggcgccggc tacgtcgtcg ccttcccgaa cccgcacggc tcgaccggct
    58621 gggggcagga ctacaccgcg gcgatctcgg gcgactggga cggcaaggtg atgaaggacg
    58681 tcctcgccgt ggccgactgg ctctccgtac agaagtgggc cgacgaggag cggatggggg
    58741 cgatgggctg gtcgtggggc ggctacgcga tgatgtggct cgccgggcac gaccacccgt
    58801 tcaaggcgct cgccgcgatg atgggggtct acgacctccg ctcgatgtac tcgatgacgg
    58861 aggagctctg gttccccgag tgggacctga aggggacccc ctggcagaac ccggaggggt
    58921 acgcgaagca gagcccgtcg tcgtacgtga cgaagttcaa gacgccgacg ctcgtcctga
    58981 ccggacagaa ggacttccgc gtcccctaca cgcagtcgct cgccttcttc accgacctcc
    59041 agaagatggg cgtgccgtcg cgcctcgtcg tcttcgagaa cgggagccac tggcccggct
    59101 ggtacgacat ggtcctctac tactcggccc acctcgactg gttccaccgc tacctcggcg
    59161 gtgcgccgtc gccgtacgac ccgaaggcgc tcgtccggaa cgacgtcttc gggaaggaga
    59221 aggacgaggc gaaggggccg gcaaaggacg aggcgaagcc cgcgccccgg aaggccgtgc
    59281 cgccgaagcc ggaggagaag aaggtcccgg cgaacccgta gccgatacgg gagccacgga
    59341 gtcgacggcc gcgtcgcgag gcgcggccgt cgtgtttcgg accgcacccc ggtgagcccg
    59401 ccgctcccta tactggccgg ccaggaagga gctccgcggt cgccatgacg atcttcgaga
    59461 aggggctcgt cacgatcctc gtcgtcgacg cgctctccat cctcatctcg atcccgctcg
    59521 tgctccggaa gatcgggccg aacgtcgtct acggctaccg gacgcgcgcg acgctctccg
    59581 acccggcgct ctggtatgag gcgaacgcgc tcttcggccg acggctgatc gcggcctgca
    59641 ccgtgtcggc tctcgctgct ctcttcctcg cgcgccccgg cctcctctcg cccgacgcgt
    59701 tcctgcccgt ctcggtcgtc ctcctcggcg cgccggtggc cgtggcgggc gtgatgacgt
    59761 cgcggttcgt ggcgcgcgtc acgaagggct gacggccggt cggctatcct ctcgggccag
    59821 ctcgcaggac aagacccctc gagagactcg cgcaggaggc tccgtattcg acgtaccgca
    59881 ctcgtcgcga tcgcgttctg ggtcgcgttc ggcatcgcga tggcgaccgt cgtcgcgaac
    59941 gcgtggagcg cccgggccgg ccgctccgac gcggcgggcc gcgggatggc gcgggcgttc
    60001 gcggtgctcg tcctgccgtt cccgctggtg gggctcgcga tcttcctcct cggccggtcg
    60061 acgggcgcga aggcggccgc cgcggcggtg gcggtcgcgc cgctcctcct cacgctcgcg
    60121 ctcgaggggg ccggccggct ctccgccttc gggaaggccc gcgccggagg cgcccgggcc
    60181 cgtttccgcg agcccggggc gcgggcgctc gcggcggcga tcgaggaggg acggctcgag
    60241 cgggtccgct ttctcctcgg gagcgtcgac cccgacgcgc cggggacgaa cggcgaatcg
    60301 ctcctcggct tcgctctccg gaaacggcag ggcgacgccg cgctcgagct cgcgcgacgg
    60361 ggcgccgacc cgctgcgcgg gccggcggga gagccgaacg ccctccgcct cgcggcgcag
    60421 gagcccctgt tcgtcgaggt cctgcgcgcc ctcctcgcga acgggacgag ccccgacacg
    60481 gtcctgccgg acgacatcga ccgcccgacg cccctcgtct tcaccgcgat gaacctgaat
    60541 gcctggccga acgtgagggc cgtcgtcgag gcgggggcgc ggctcgacgt cgtcgacgtc
    60601 aacggccgga cgccgctcgc gcgggcgatc ctctggcggg cgtggaacga ggcgcggctc
    60661 atggtcgagc ggggcgcccc ggtgacggcc gggctgcggg ggccggcgag cctcgaggag
    60721 gcgttcgcgg caacgcgacc tcccgaggcg ggcacccccg agcgcgccgc ctgggacctc
    60781 ctcctcgccg ctctcgccga acgcggattc ccggaaccgg ccggcgcacg ctgaggtcgt
    60841 gaccctggcg gcccggaccg tccggttccg gacagcggcg tccggcgccg gacgcgtccg
    60901 cgctctgccc cgccctgctc cgcgcggcac atacgcttct ccaggaatga cttggggacg
    60961 cgacgcgggc tggcaccccg attgcgcttc ggcagggcgt ggatcgcccc ctcgaagacc
    61021 gtgaggtccg aaccagacgc ttccggaagg tgcgaaccgc cctcgccgcc gccgcccttc
    61081 tcgtcggggc gtggctcggc ctcggcgccc tcctccggcc ctcgctcacg ctcgcccgcg
    61141 taaggaccgg gagggtcgac gtcgggccga tgacggcgac gctctcggca ccggggacga
    61201 tcgtgccgga gtccgagcgt gtgatctcga gcccgatcga ctcccggatc ctccgcgtcc
    61261 tcgtccagcc cggagccgcc gtgaaggcgg gaacgcccct ggtcgagctc gacgcgacgg
    61321 ggccggcgct cgccgccgcc cgcctccgaa aggagctgcg gctccgagag gcggcggccg
    61381 agcgcgccgc gctcgcgctc gaggggacgc tcgcctcgct cgagagccgg gtggaggtcg
    61441 ccggcctgac gctctcgacc tggggcgcga gcctcgagcg gaaccggaag ctcttcgccg
    61501 agggactcgt ctccgaggag ctcctgcgcc agtccgagct cgacgaggcg cggacgcgcg
    61561 tggagctcgc ccggctgaag gacgagatgc gcctcgcccg gcgctccgcc gaggtggagg
    61621 ggaggtctct cgagctcgag ctcgcgaccg cccggagcga ggccgcccag gccgagcgcg
    61681 agctcgccct cgccgccgcc cggagcgagg cggccggcgt cgtgacgttc gtcgcgacgg
    61741 gagagggggc ggccgtacgg aagggggacg tcctcgcgcg cgtggccgac ctgacggcgt
    61801 tccgcgtcga cgcgctcgtc ccggacgtcc acgcgtcacg cgtggcgccc gggcaggagg
    61861 ctctcgtgcg gatcgacgac gtacgcctgc cgggtacggt ccggcgcgtc gcgcccgagg
    61921 tgcgggacgg ccaggtcgcc gtgagcgtgt cgctctccga gagcgcgcat gcgctcctcc
    61981 gaccgagcct gcgcgtcgac gtggagatcg tcaccgggcg gaaggagcgg gcgatgcgcc
    62041 tgccgcgcgg ctccttcgcc gcggccgaag ggggcgtctc ggtctgggtg ctgaggggag
    62101 acgaggcgct gcaaagcacg gtgcgcctcg gcctctcgaa cgcagagcgc tacgaggtcc
    62161 tctcgggcct cgcggagggg gacgaagtga tcctgtcgga gatggccgat ttcgcgcacc
    62221 tctcgcgggt gcgggtgaag ggacgtcagg gggggacgcg atgatccgac tttcgaacgt
    62281 cgagaaggtc taccggacgg accggatcga gacgatcgcc ctgtcgaacg tgagtctcga
    62341 ggtggaggcc ggggagttcg tgtcgatcat ggggccctcg ggctccggga agtcgacgct
    62401 cctgcacgtg atggggctcc tcgacgcgcc gacgcgcggc tcggtggagc tgaacggacg
    62461 gaaggtcgac tccctctcgg acggggcgct cgcgaaggtg cggaacgccg agctcgggtt
    62521 cgtcttccag acgttccacc tcgtgcccga cctctcggtc ctcgacaacg tcgaggtgcc
    62581 gctcctctac cgcggcggcg gagcgcgcga acggcggcgg aaggccgagg ccgcgctcgg
    62641 gcgggtcggc ctctcggccc ggacgcgtca cttcccggcg cagctctcgg gcggccagca
    62701 gcagcgcgtc gcgatcgcgc gggccatcgt cggctcgccc cgcgtcctcc tcgccgacga
    62761 gccgaccggg aacctcgaca gcgcgatggg cgacgaggtc ctcgcgctcc tctcggagct
    62821 gaaccgcgag gacgggacga ccgtcgtcat ggtgacgcac gacgagcgcc aggcggcgaa
    62881 gacgcaccgc gtcgtccgcc tcttcgacgg gcggccggtg agctgacgat gctcgcgcac
    62941 acgctgaaga ccgccgtccg cgtcctcctc cgccggaagt tcttcaccgc ggtcagcctg
    63001 ttcggcgtcg cgctgacgct cgtcgtcctc gtcgtcgcgg cggcccttct cgaccacacg
    63061 atcggcgcct atccgccgga gacccgctgg ccccgcagtc tcggcgtctt ccgcgtctcg
    63121 ctgaagggag aggggtggac ccgctccggc ggcgcgggcg gccggttgct cgagacgtgc
    63181 gtgagggacc tgccgggagc ggaggtgacg acgttctact cgcaggggga ccgggaggtg
    63241 acctaccggg gcggcgagcg cctgccgctc tgggtgaaac ggaccgacgg cgcgttctgg
    63301 cgggcgctcg acttccgttt cctcgaggga gggccgttca ccgaggccga cgacgccgcc
    63361 gcgaacgccg tggccgtcgt caacgacgcg acgcggcgga ggctcttcgg gagcggcccg
    63421 gccgtcggga ggacgctcgt cctcgacggg aggacgttca cggtcgtcgg cgtcgtcgcg
    63481 gacgtcccct tcctccgacc ggtcgccttc tccgacgtct ggctcccggc ccgcaccgcg
    63541 cggagcgacg actggcggcg cgcgtgggtc gacgactgga aggcgttcgt cgtcgcgaag
    63601 agcgaggcgg acctccccgg catccgggag gagctgaagc gccgcgtcgc ggccgtggac
    63661 ctctccgacg agaagccgtt cgacaggctc gaggcggagg cggagacggt cttcgaggcg
    63721 atcgcccgcc tccccctcgg gaaccggcca gaccgcgcgg cgctcctcgt cgcccttctc
    63781 ggcggcctcg ccctcgcgtt catgacgctc cccgcgctga acctcgtgaa cctcgcgctg
    63841 agccggatcc tcgagcgggc cccggaggtc ggagtgcggc gggcgttcgg ggcgacgcgg
    63901 acgcgcctcg ccctccagtt cgtcaccgag aacctggtcc tcgcgctcgc cggcggcgga
    63961 gtcgggctcg tcctctccgt cggcgtcctc gcggcgatcg acgcgagcgg cctcatccct
    64021 tactccgacc tctccctgag cccgagcgtc ttcctccagg ggctcgcggc tacctgcgtc
    64081 ttcggggtcc tctcgggcgc cctcccggcg ctccggatgg cgcgcctcca cccggtcgaa
    64141 gccctccgcg gaggaccgcg atgacccggc acctcctgaa gctcgtctgg aaccgccggc
    64201 ggcaccacct cctcgtcggc ctggaggtcc tcgcctcctt cctgatcgtc ttcgtcgtcg
    64261 ccgtcctcgc cgtgaagacc gtcgggaacg ccgcccggcc ggccggcttc gtctccgacg
    64321 acgtcaccgt cgtcgaggtc ggtctccgga agcggggggg cggcccggtg gtcgacgacg
    64381 gcgcggaccg ggagcggatg cgccgggccc tcgaagcgct ccggcgcctc gaggcggtcg
    64441 aggcggccgc cgccgcgttc ctcgtcccgt acgggacggg acgcgccgtg aacagccaca
    64501 ccgtgaacgg ccggacgctc gagtacgact acagcgacgt cacggacgac tacctcgcgg
    64561 tggcgcggct gaagctggcg gcgggtcggt ggttctcacg cgaggacgac ggccgcccgt
    64621 accgccccgt cgtcgttacg cgcgagctgg cgcgcgaggc gttcggagcg gaggacccgg
    64681 tcgggaggga gattccggag ggcggggtcg agcccgggac ggcccgcagg cggagccgcg
    64741 tcgtcggcgt cctcgaggcc taccggcccg ggagcgacta cgccgcgccc gcgccgttcg
    64801 ccctcttccg cgcggacctc gcgaacccgg cggacgacgt cccgtcgcgg atcgtcgtcc
    64861 gcctccgtcc cgggacgccg cgcgccttcg aggagacgct cgtccggagg ctcggggcgg
    64921 ccgtccccga gtggagcctc accgccgagc cgctcgccgt ggcgcgcgag gcggcgaacc
    64981 gccgctggac cgtcccgatc ctcgccgcct cgctcgtggc gggcttcctg atgctcctcg
    65041 tcgcgctcgg gctgacgggt gtgctctggc agaatgtcac gcgacgtacg cgggagatcg
    65101 gccttcggcg cgcgaccggt gcgacggcgc gccaggtggc ggtgcaggtg gtgatggagg
    65161 tctggatcgt gacggcgctg gcggtgctct cgggttgcgc ggtggcgctc caggcgccgc
    65221 tcctcgtccc gggcggcttc gtccgcgcgt ccgacgccgc cgcgggggcg gcggccgccg
    65281 ccgtcctcct cttcgccctg accgggctct gcgcgctcta cccggcgtgg ctggccacgc
    65341 gcgtcttccc gtccgaggcg ctccggtacg agtgaggacg tgaaggacga cgccgccgtc
    65401 ctcgtcgtcg acgacgactc cgccgtcgcg gcgtcgctcg tcctccttct ccggcaggcg
    65461 gggtaccgcc ccgcctccgc ggcgacgccg gaagaggcgc tcgcgcggct cgccgccgcg
    65521 ccggcggacc tcgtcctgtc cgacatgaac ttcgcccgag ggacgacggg cgaggagggg
    65581 ctcgccttcc tcgcggcgct ccgcgagaag cacccggggc tccccgtcgt cctgatgacg
    65641 gcctggggct cgatcgagct cgccgtccgc gggatgcgcg gcggcgcggc cgacttcgtg
    65701 acgaagccgt ggagcgccga gcggctcctc tcgaccgtcg cgacggcgct cgcgctctcc
    65761 ggagtcgggc ccgcccgcga ctccgacgtc ccgtcgcgcg aagagctcga cgccgccgct
    65821 cccgccttcg cgccgctcgc agggcgggac ccgaagctcg tgcggctcct cgccctcgcc
    65881 gcgcgcgtcg ccccgacgga cgcctcggtc ctcgtgacgg gcgagagcgg caccggcaag
    65941 gagctcctcg ccgaggcgat ccaccgggcg agcccgcggc gggcgaagcc gttcgtgaag
    66001 gtgaacctcg gcggcgtccc gacgacgctc ttcgagagcg agatgttcgg ccacgtgaag
    66061 ggggccttca ccgacgcccg gcaggaccgt gccggccgct tcgccgcggc gcacggcggg
    66121 tcgatcttcc tcgacgaggt gggcgagctc gagccggcgg cgcaggtgaa gctcctgcgc
    66181 gtcctccagg accgaacgtt cgaggtcctc gggacgagcg cgacgcggag cgtcgacgtc
    66241 cgcgtcgtct cggccacgaa ccgcgacctc ggcgaggcgg tcgcgcgcgg cgccttccgc
    66301 gaggacctcc tctaccggct caacctcatc acgctccgcc tcccgccgct ccgggagcgc
    66361 cccgacgacg tgccgttcct cgccgcgcgg ttcctcgcgg agctctcgcg gcggcacggc
    66421 cgcccgctcg cgctcgcgcc cgacgccggc gagtggctcg cggcgcgccc ctggccgggg
    66481 aacgtgcggc agctgaagca cctcgtcgag cggagcgtcc tcctgaccgg gaaggagacg
    66541 ctcggagccg ccgacttcgc gttcgtcgcc gggatggacg agggggcgcg gcgggaggag
    66601 cccgcgacgg cgcggacgct cgaggcgctc gagcgggaag cgatcgcccg gtgcctcgcg
    66661 cggcacggcg gcaacctctc ccgcgcggcc gaggagctgg gcctctcgcg cggggcgctc
    66721 taccggcggc tcgagaagta cgggattgcc ccgtgagctg gtcggcccgc gcgctcctct
    66781 acctcctcgt cctccacgcc gccctcgccc tcctcgccgt cccgctcctg cgcgagagcc
    66841 ccggctggct gctcgccgcc gaggccgcgt tcgtcctctc cgcgctcgcc ggggcgctcc
    66901 tcgtccgccg tctggccgcg ccgatggagt cgctccgcga cggcgccgag ctgcttcgcg
    66961 ccggggagct cgggacgcgc ctgcgcccga gcgggacgcc cgagctcgac tccctcgtcg
    67021 ccctcttcaa cgagatgctc gcgcgcctcc agtccgagcg ggtgcgccag gaggagcggc
    67081 aggcgttcct cgagaaggtc ctcgaggcct cgcccctcgg cgtcgtcacg ctcgacctcg
    67141 acgggaaggt cgacgtcgtc agccgcgcgg cgacggggct cctcggcgcc gacgccggag
    67201 ccctccacgg ccggcgactc gtcgaggcgt cgggcccgct cgccggggcg ctctccgagc
    67261 tccctcccgg cggctcccgc gtcgtccctc tcgggggcgg ccgtcgcgtc cgcgcccgtc
    67321 gctcgcactt cctcgaccgc ggcttcccgc ggacgttctt cctcctcgac gagctgacgg
    67381 aggagctgcg gctctcggag aaggcggcct acgagaagct gatccggatg acgtcgcacg
    67441 aggtgaacaa ctcgggggcc gccgtcgggt cgctcctcgg ctcgctcctt tcgctcgcgc
    67501 cgcgcgtccc ggaggaggac cggaccgact tcgtcgaggc gctccgcgtc gcaaaggaga
    67561 ggaccgagcg gctgaactcc ttcatgcggt cgctcgccga cgtcgtgcgg gtgccggcgc
    67621 cgcggctggc cccgaccgac ccgaacgctc tcgtccgctc cgtcctcctc cttctcggac
    67681 cgctctccga gcggcacggc gtcgcgctcg ccctcgaggc cgacccggcg ctccgccccg
    67741 tcccgctcga cgcggccctc ttcgagcagg ccctcttcaa cgtcgtcaag aacggcatcg
    67801 aggcggccgg ccgcggcggg agcgtgacgc tccggacctc ggcggaggga ccgggacgga
    67861 tcgaggtcgt cgacagcgga cccggcatcc ccgccgacgt cgaggcgcag ctcttcactc
    67921 cgttcttcac gacgaagccg gaagggcagg gcgtcgggct gacgttcgtc cgcgaggtcc
    67981 tcgcctccca cggcttcgcg tactccctcg ccggcccgcc cggcggcccg acccgcttcg
    68041 acgtccgctt cggcgcctga agcgcagagc cgacgagcac gccccacgaa gggcggcgcg
    68101 aagcccgcgc cgccgagggc cgtccggccg gcgtttcacg tgcctcgcgg agaggtcgtg
    68161 ggggcctcgg cgcccccgcg acgcgcctca cgacgccggt acgaggcccg gcccctccgg
    68221 ctccccgtcg acctcgctct cggtctcctc cgcccggcgc gtccgggcga cgaggacgta
    68281 gatggacgga acgacgagga gggtgaaggc cgtgccgatg aacatcccgg tgacgaggac
    68341 gatgccgatc gagttgcggg cgcccgcgcc cgggccgctc gcgaagacga gggggaggtg
    68401 gcccatgatc gtcgccgccg tcgtcatcag gaccggccgg agccgtgtgc tcgcggcctc
    68461 gacgacggcc ctcaccttgt cgatccccgt ctcctggagc ttgttcgcga actccacgat
    68521 caggatcccg ttcttcgcga cgaggccgac gagggtgatg agccccacct ggctgtagac
    68581 gttcagcgag gtgaaggaga ggaacgagaa gaggagcgcc cccgcgagcg cgagcgggac
    68641 cgagcccgcg aggatgatga acgggtcccg gaagctctcg aactgagccg ccaggacgag
    68701 gtagatgagg atcgccgaca ggaggagcgt cccgaggaac ttgctcccct cgacgcgcag
    68761 ctggcgcgac tcgcccgcgt agtcgagcgt gaagcccttc ggcaggatca tcgccgcctc
    68821 cgcttcgagg aaccccaggg cctcgtcgag agagacgccc ggcgcggtga cgccctggat
    68881 ccggaccgcg ttcagctgct ggaatcgctt cagctccctc ggctcggtcg tcgtcttcag
    68941 ggacgcgaac gtcgagagcg gcacgaggcg cccgtcggca cccgagacgt ggatctgctc
    69001 gagctggtcc ggggtgaggc gctccgcgcg cttgacctgc gggatgacct tgtagctccg
    69061 tccctggatc gagaagcggt tgacgtagtt ccccccgagc atcgtcgaga ggtcccggcc
    69121 ggcgtcggcg aggtcgacac cctgcgcccg caccttgtcg cggtcgaaga cgacctcggc
    69181 ctgcggctgg tcgaacttca ggtcggcgtc ggcgaacatg aagagtccgc tcccgaacgc
    69241 cttgccgacg agctggccgg cgaactccgt cagcctctcc ggctcggcgg tcgaggcgat
    69301 gacgaggtcc accgggaagt cgcctcctcc gggaagcgcc ggaggcgtca gcgagatgac
    69361 ccgcaccccg gggatcttcg agagcttctg cgacgcctcc acgtggagct cggcggtcga
    69421 gcgctcgcgc tcgctccagg gcttcgtgac catcccgccg aagccgccgc tcggggaggt
    69481 gatctggaag atgctgtcgc tctcggggaa cgagcgatag acgtcgtgga cctgcgcggc
    69541 gaagagcttc gtctggtcga gcgtcgcgtt cggcgcggcc tggatgatcc cgaagacgac
    69601 gccctggtcc tcgtggggcg cgagctcccg ctgcgagaag aggtagaagg ggacgacgag
    69661 gagcgagacg aggacccaga gcgtgaggac gacgggccgg tagctcagcg tggccccgag
    69721 ctggcgctcg taggtcttcc tcacgaactc gaagcggcgg ttcacccagc cggcgaagcc
    69781 gcgctcggag tcgcccgcgc ggaggagctt ggcgcccatc atcggcgaga gcgtgagcgc
    69841 gacgacgccc gagacgagga ccgcgcccgc gagcgtgaac gcgaactcgc ggaagagcgc
    69901 cccggtgagc cccccctgga ggccgacggg cgcgtagacg gccgcgagcg tgatcgtcat
    69961 cgcgacgatc gggccgacga gctcgcgggc ggccttgacg gccgcctgga aaggcgtctg
    70021 cccgagctgc aggtgccgct cgacgttctc gaccatcacg atggcgtcgt ccacgacgag
    70081 gcccacggag aggacgatcg cgaggagcgt caggaggttg atcgtgaagc cagcgatcgc
    70141 catgaggaag acggcgccga cgagcgagac ggggatcgcg acgacgggga tgaggacgga
    70201 ccggaaggac cccaggaaga ggaagatgac gaggatgacg atgaggagcg tctcggtgag
    70261 ggtcttcagc acctcgtcga tcgcgtcctg gatgtaggcc gtcgagtcgt aggggacacc
    70321 gaccttcatc cccgccggga gctgcgcctg gatctcgggg atcgcctcgc gcaccccggc
    70381 ggtgacctcg agcgcgttcg ccgtcgggag gacccagatg cccatgaacg tggcgtcctg
    70441 gccgttgaag tggacgtccg actcgtagtc ctcggcgccg agagtgacgt cggcgatctc
    70501 cccgagccgc acgatggagc ccttctccct cttcacgacg agctggcgga actcctcggg
    70561 ggtctggagg ttcgtgttcg cgacgaggtt caccgagacc atcgagccct tggtccgccc
    70621 gagggccgag aggtagttgt tgttcgcgag ggcctcgcgc acctgggagg ccgagaggcc
    70681 gagcgccgcc atccgctcgg gcttcagcca gatgcgcatc gcgaagttgc ggctcccgag
    70741 gatgtcggcc cgctggactc cggagatcgc cgagagcttc ggctggacga cgcgcgtcag
    70801 gtagtcggtg atctggttct ggtcgaggtc cgccgaggag aagccgatgt acatcgccgc
    70861 gaactgcgag tcggcggtct cgagctcgat gacgggcgcc tgcgcctcgg gcgggaggtc
    70921 gttgcgcacc tgcgcgacct tggcctgcac ctgggtgagg gcggcgttcg tgtcgtagtt
    70981 caacctcagg tggacggtga tcgtgctgac gccctgcgcg ctcgacgatt cgatgtagtc
    71041 gatgccgtcg gcgctggcga tgacccgctc caggggtgtc gtgatgaagc cccggacgag
    71101 gtcggcgttc gcgccgacgt aggccgtcgt caccctcacg acggcgatgt cgctccgcgg
    71161 gtactgcctc acggagagcg accggatcga ctgaagcccc gcgatcgtga tgacgaggct
    71221 gacgacgacc gcgaggacgg gacgcttgat gaagaggtcg gtcagcttca tcacgagtcc
    71281 tgcggcatcg gggccgggtc gttcccgggc cggatccggt tgtcgacctc gacggccgcg
    71341 cccgggcgaa gcttgaagac gcccgaggtg acgacctcct ggcccggctc gagccccgac
    71401 aggacggcca cctggtcgcc gcgggacgct ccgagcttca cgaactgctg ctgcacgcct
    71461 cggtacggcg tgccgtccgg gcccttcagc tgcgccacga cgaagaccga gtcgccgtag
    71521 ggcgcgaagc tgatggccga gcccgggagg gcgatcaccg gcgtcgacgt gccgaggtcc
    71581 gcctcgacct ccacgaacat cccgggacgg agtcggccgt ccgggttcgg gagggtcgcc
    71641 tggatctcca cgttccgcgt cgccacgtcg acgaccgcgt cgatcgccga gatccgggcg
    71701 acggaccgcg tctcctgtcc cgacgcgtcc tgcgatctca cctcgacggc gccgcccttc
    71761 cgaaggcgat tgacctcctg ctgcgggacc gagaaattca cgtggatcgg atcgatcgac
    71821 tcgagcgaga cgatcttctc cccggccgcg aggtactgcc cgaggttcgc ccggcggatg
    71881 ccgaggaccc cggagaaggg cgcccggatc gtcttccgcg caatcgtcgc ccggatctct
    71941 ccgacgcgag ctgttccctg cgtgtgctcc gcgagcgcgc ggtcgtagtc cgcctgcgac
    72001 aggacgccac catcgcgcag accgcgggct ctttcgaggt tcgcccgggc gagctcgagc
    72061 tgggcctcgg cggcggcgag ctgcgcccgt tcctgccgcg tatcgagctc cgcgaggacc
    72121 gctccctgcg aaacggggcg ccccgattcg aaggtaatcc tgtcgacgac gccggggagg
    72181 tccgcgctca cggagactcc gtggacggcg acgacggtcc cgatggcggc caggctggag
    72241 ggccagcggt cctccctggt gagggtcgtc gtgaccgcgt ccggcggggg acggaacgag
    72301 gcgccctgcg cgccggcggc ccgcagctgg ccgtacttca gggtcgcgac cgcagcgacg
    72361 aaggcggcca cgagggagag catcagcgac atcctcttct tcatgggaga gtccctccag
    72421 gctcgccggt cgggaggctg cgccaaaggc cggagccggg tcggcagagc cgatccccgg
    72481 cgcctgcgcg cctgatcccg aacacgagac gaagggatta cgccagaacc tgcgactggg
    72541 tttcagggag cggcgacggg tcgtcgcgcc ttctcgtccc gttcccgaac cgccatcgcc
    72601 gggctcgggc cggacggatc agcaactatt tcgctcatct caaacgactt acgtgacaga
    72661 cgtcgcccct ccccggggaa gagagatgcc gggacgtcgt aggtcaggcc gagcgaccga
    72721 ggagccccga ggcgaaggag cggaagagcc tcgccccgtc ggcgttgccg aggacctcct
    72781 cggaggcgcg ctcggggtgc g
//
LOCUS       DAOONL010000007        71513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_222_length_71513_cov_14.121148, whole genome shotgun sequence.
ACCESSION   DAOONL010000007 DAOONL010000000
VERSION     DAOONL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 71513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71513
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_71513_cov_14.121148"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..3769
                     /locus_tag="PLP50_02150"
     CDS             11..3769
                     /locus_tag="PLP50_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50380.1"
                     /translation="MRGIPVEPSCLGQQWQQAVFDNGKTLVISETTQGRLDSLTLIRV
                     GRVAMLWNHARHVIVYERTTRTAPRYTHGEQPDDFEGLAALRKVREYVEILQPRRDYP
                     DFPAGLPYAGPLQRSTFETTVIPVKSTWGRDVENGWVMPLRGPLSPEEEEAFPVPKVF
                     SELARAAGKGEGRIAHRVTNPDVLHFFTSTRDEDGGDTDAWPSFPDVDFPVTERPSQP
                     PLPSAPRFSDLSIQPDAVGVGYGQRRFTVDLAPAEEAANLMHGRPVPGIEARVHNLSL
                     ARGKPPVLAPCDSLEALAGRPFAEAEAVLRDGIAELSGSLARLDRGGDATLGQIGQSW
                     SEAQALHKNLANRATALRGTLEKQRSDLEKYAFSWRKEQDLWLERHGASAKRLVEREL
                     KPAVETALQTAGDLVGKGQDALKQSLTIDLEGLCRQARGHLDEVSLLPELALEPLRRA
                     SRDLRARTEATLAGARVRIDALLSDLEARYAAEGARALEAELFEGLFDVRERLVAFQS
                     QARRLGSEALGVWFGLQRDAAGKPNAVDQIADAVNIACGNVLRELELAIGEIPPLEVG
                     VDWASIRALFGSLEVDVAPLFDKIDAHISKLANVDIEIGGKRYGLLPKDGRSRWKELL
                     DPGASIEKACGAAHDIIEKLKFENVASDLATAHGVLQSFVEKAGEKLATSSPELGAFL
                     DAFGKIESWNSTLGETGDINQPLDAIESALEELGSVLASAGSPVADAAAAAGRAATLL
                     GEHLDALGRRVEGAVLAEARALGRLLPSDGIHLELTRALAEGPVTDALRCTRRLLGYT
                     YDAASALLQATRSSALFNDLGPGVLNSLGVRLPFDRLGDRLLPRLDGLAIGDLFPDFG
                     GLKLAHFLKGLRVPSDPAQEFGWVRVTHGFDKDRLLGWAEVVVDKRFEEEPSLFDLPP
                     FSLRLVRPHFSATSRFEADATGGRHQTSRASLAADWKLCLSNQPVITIKKGVLRHESD
                     GGFGFDFKAKDLELAEPLRFVTDAVSALLPQAEGLTVSPVPPGGISAELNLPLPDLAA
                     GAFTLTGITLHSHFDLLIGQGFEVRAGLWLSKPDRPFGLAVLFLGGGGWFGVDVSYRP
                     PDVFETRLSVGISAGAFVSLNFGVAKGSAGVLFTAGLDFYRSDRTGARGDTSVSLGLL
                     VWGEFSIVGIASAHLRMVLRVTYRNGEMKGHGRMSVRIKICWCFTLEVESEIDLPFDR
                     GGAPRAMPAGTKALSAPRLGTAASVTPVATIERAVDLHLGSLDLVEAA"
     gene            3766..9738
                     /locus_tag="PLP50_02155"
     CDS             3766..9738
                     /locus_tag="PLP50_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50381.1"
                     /translation="MSTLRVILSLHAVGPRDHLLDLDTIPFALQLSAGFEGDVPDAAR
                     KAWATAALPWEFCLPLPGGDDGRRDQLELRLYRGVTNDLNTIGVVRAHRLGYGPRHPF
                     LSALAQGVVDRFVNDQMVLQRLALSWDANKPVAASETSPHWGAIIQSLSVLPPEIASG
                     VSAAWYFEVPRDALFKNVGGTFTPVVPYFIAAPVRLRDGRHDREASLQGKREGPSEVT
                     FEYEDVKALPAIRAIGPAFPLASLGAEAGGGSFSLGDYWLSVKDSRGVALADVDGVLA
                     QALEPRVMAAEVDQDVVGRVLRIRDGGAPRRATGEQAELFRTLAGQTSLEDAIERDLM
                     AESVLAGLKNAGTDDARTVLRRLLGDALSKRWKDLAGSTVAAARERGLLRDGEMWELD
                     DAGADELARPLVEHAVARNDTAVVRTALPGEGLDFVAGASEHRLVHEENSSFGSNREG
                     AYSVVSGVGVLVRRWNDKATAESRPWRLVTAGVPHLASRGEWFELEPLPLTAGFLNGV
                     LRSDRTYLGEPMRTPSTLVKAHAYRAPENEKRPGDPTALEDLEFLSIAHYRAANEAEA
                     PNAGWASAPPLRYGDGYDVAAFVVDKAGGLPVEMARWREQPVTVDWSSLRTLAPRGFA
                     RITFLRRVPVGAVNVLPGAKEDAMERAAWPAIPDGVALRSREWYRGANLGSGDSPALL
                     LSTHPDWRGTLRDYSFRVEPPRLDEHTLLRWAVPALGSKDAVTETKRLRDAWVRILAW
                     RDAETARDPEGKTPRYSVPGLPHDPAVTALGFRLTKVDASGAGAEPKLVVVPLEERAP
                     FEMQPIQVTVAASETTSISSANASVTVTLKEGDFAAFEVCALVLETDFSRFEGEAMKE
                     LVEARLWPHAGTAYRAFCGTTLLLEGATNALPEAEDLFGALGLQEREGRIHVTLQGRP
                     GDLHFVDTVEIERERWVWRNRPLVMSASSDEGPEERRRRLASGLPNALSDAAMRDVDG
                     RVREFDAIAGPDRGFVDRGTVRLRLPRDGDGGGAPNALLLVDERDGSPSGDYLSYGLR
                     VRSRYAPVLPPNEREVLAVRAASESPANEPDDFTRRWRTRRRVVGTYRGGPLKPPKIL
                     AVVPLTRDLTDEPGRDTHTPGVPPHLVLVDEVWFREYGPGERLEARLVLETREIGEAE
                     DGARPYRTGPMPDHHLAPPAGTETKETGTEVSEPNRYYRHRMRSDTGDEEDPILLDVF
                     GPFGYTVDRSGNEALANATAFLVYLPNDVGPHWCSYVRFSRVLDGPGCPEPRRSERSG
                     LYPLYTRPDAGRLASGDEGGLLLAGGIARLTGGLKLLLDPIAVPSGKPAAASETLARR
                     NYRYLLLVSRAVVDAAQGVEVELPQNLYRLTGTKLNKDRVEFREGQPLMATPIGDQAR
                     PGEYRGRILEVLVNGVYEDGRAPLDDAKNVKDFLRRLLPTPKEPREGDRLPYVSELED
                     APGLLLPISCAFPVRVEDEGPPEALDPKVYMTRPPKPTPNDMVTLRFRAVSTGAKLEY
                     GPHAALPGFTQAFEVRVEDRRELPSTVHLEGSEVTPLRWACGSLIWRTFEDVTSEGFL
                     EYGLDLWKETQVREFPDDELRWTMPHRIPASILPGESWTQEVEELVRDRDGNPKGPRR
                     YTRTVTFEGFEALVPSGEAFNECARVREDFLAPGDGGAEWIVRWFAPGIGEVRSEDSG
                     GSVWELLATKAADPEAEKSVGPPPDRPALVGRSLPLSLKNGESTMTKKSLSRKATPRR
                     GSGVISTKTGKPTSLRGTRAPSDRKVVRKTAARIRRRVSLTAGLESASLALAAANVPT
                     ASKKPNERVNAFWAIAEAVAQELDQPSVRYRAKVFLLRVAWHESARLTTRLQGGGGPA
                     RGLLQFETIRAEEAGGYAEQKGYLKDLSRVSSRTEAALKGAFAALHGHATYPSGNLIE
                     DLLSGNANANDHFCVLLGRIALKRGAKALPDEADLGATADYWYCQWNHNFSDKVCQAS
                     KNPGDPNNADRNDKIQAFLSSCAALAEHLPSS"
     gene            10197..10820
                     /locus_tag="PLP50_02160"
     CDS             10197..10820
                     /locus_tag="PLP50_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50382.1"
                     /translation="MSSLIPTVAAIAALMAPQFGGMDLAAAQKWEAAKVVHYRIVGVH
                     DGREMVVFGDYEGKADVLDRVTVELDWDVRKRKVIGAVKVTDAASTLKNVKSDGTNCP
                     PPQLNGAYEHFKYVSHSMSTNDQIVIKGSRTYPAAKVSNYPASCSMRSISGGREDTIL
                     GVAIVAPEVLGMPIMKDGPVVVADDRKTFSVKANDNWVWTYTPTVTQ"
     gene            complement(10848..11582)
                     /locus_tag="PLP50_02165"
     CDS             complement(10848..11582)
                     /locus_tag="PLP50_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50383.1"
                     /translation="MKTPRSIRSASLPVLLASLLLAGGTAVAQTQMTPEQEKVYRDMI
                     AASGANPDDVMGAKKQVDDSRKWTGGPIDYHAVGVYSGKPQITSDPGKGSGVADVTDQ
                     VILDFKWDLAASGLVGTPRIQNSKSALKNPRDLEPKCLPPVLKGEYEHADVLSVKNGL
                     GGALELDVRTTYPLVEVVQFCTGARKPIPGAAKTRRAELVVPSPVTFGMKLPASSKVT
                     VSPDRKSLIYRDADWTWTFTPKPRNV"
     gene            complement(11661..12920)
                     /locus_tag="PLP50_02170"
     CDS             complement(11661..12920)
                     /locus_tag="PLP50_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50384.1"
                     /translation="MTIRALFPPRSSCARASTLVALIALALGASPAAADEANGVRFAV
                     PEGWKATERNGLPVIAPSRLAPGEVMSALVIGVQAASGTPAAQVAQVADGVNADAKVV
                     GSGRLVTTDRGAVGTFHIQSFDVESADLGAHTRMVVLIVGGNRRAVAMLVFSTNAVLQ
                     KYGAGVQALIDSLAISPSAVPAAPVPASRPAVPAAPSASSRIPTGETPDLFPGSPGWL
                     PSGRGVRIPPARLVEGRPQGLWWHPQARSGRTVAMLTLFLPDGTRASSPRPGAGDLFD
                     VEGQKRQRGATGVGTFEAAGGRITQSYDGFVKTDTFRTGSDADGAWIEIGAGRHRPLA
                     PPAAKGLVGTWSGAGGRYVFRPDGTYESGHVSASSELTIAAGARGTYVLDGYLLALRP
                     ADGPGWITTIGASGASCLVIGPTLYCR"
     gene            13080..16430
                     /locus_tag="PLP50_02175"
     CDS             13080..16430
                     /locus_tag="PLP50_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPA50385.1"
                     /translation="MRSSVVRDELIRALRLDLIGPEPGSEHEREELPHEPSRWYVGGF
                     LVPFEAPEEQKVDPTGQEELFAAGDENGADDADVPERASARKVFLPSSVGLSVLVAKG
                     TGALEAELTWGDYRLPKAEEGEPRGRESAWTREPRRAVVALALPEGDAKAATVAVPDS
                     DGLNLVVSGRAAGEAEALGLPKGTRSVSVFLVNDRKPAPEERKDEAVAFQVRLALRSK
                     EPLVARPNATGIDSTDWDDRVADLQYRDVFEYAVGHGVSARAEVAEDGSCREVATEWV
                     PTAAVEKVEPSRLEGVELGMEALAATGDSEALAASLAAFPARYGEWIARQRGTKLEGK
                     RAEAAAILLDEAKHACARIERGIALLKEPDVFKAFRVANRAMARAARRRSARAANVAP
                     EAVEPPRWRPFQLAFLLMNLEGIARPGSSDREAVDLLFFPTGGGKTEAYLGLAAFTMV
                     LRRLRNSGLSSAGVAVLMRYTLRLLTLDQLGRVAALVCALELEREADAALGTWPFEVG
                     LWVGRAATPNRMGAKGDDDETTARSKTLKYKKDSKRWPPPIPLESCPWCGTPFTKDSF
                     SLQPTSDRPTDLRVVCVSRRCEFRGDRPLPILTVDEPIYARRPAFLIATVDKFASLPW
                     VGDVGRLFGKGEKEPLPPPELIIQDELHLISGPLGTMAGLYEIAVDHLSTREEAGKRI
                     PPKVVASTATIRRAEAQVRALFGRSAVYVFPPPGPDRRDSFFARTVAQSEREPRMYVG
                     LSAQGRSLKVLLLRAYLALLGASQRLFEEAGGASLGEKNPADPYLTLLGYFSSLRELG
                     GTRRIVEDEVAARAARYGERRRAGEAAGAFRDRTIQQEVVELTSRESTAKVARAKERL
                     ALPFTSRDHVDVALATNMISVGLDITRLGLMVVLGQPKTTSEYIQATSRVGRDDARPG
                     LVVTLQNVHRPRDRSHFERFGAYHASFYRAVEATSVTPFSARALDRGAAAVAVALARH
                     GLAALTPAAGAGQVEAVRKDLAFVGEEMAERASRHDTEKGAEELEALRKRVKSLVDDL
                     LDSWAVLAHEKRQNDVKLAYQAKERGGPPNAFALLRDPLDPELESLPSSARKFRAQRS
                     MRDVEPSVPLVLRRLDDVEVPDAEEAGS"
     gene            16427..18232
                     /locus_tag="PLP50_02180"
     CDS             16427..18232
                     /locus_tag="PLP50_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPA50386.1"
                     /translation="MKKRHHGEIRRSQLVRTFGPGAMVDLPNHSVVVSGLDSWFPASE
                     MPTIVEERLSLKVASVLGHGKPVPLKGPPVVSDDERTPATGVPAFQFPEWFVVQVNAK
                     RRPLVHLESLDKGKYEVDRKKVPAVPVRFVQACVLGHLSDIDWRGFIHMYRGDCSRPI
                     WMEERGSSGDLADVVVGCDCGAERSMAQATRRGAESLGECAGRRPWLGRHGGERCGGE
                     NGKRQLARLLVRSASDAYFPQVLSVISLPDRGSVVRDAVAKVWENFLSLVEEAGELEK
                     EKKRPAVKAALEGLTDEEVWTEIERRRTGAPAQTEKIKEVELATLLSVKEKLGDDVHS
                     GHDFLARRLPLEATTGPMMAIERVVLVDRLREVTAQVGFTRFEAQTADIDGELSLDVR
                     TAPLAAEVKWVPAVENRGEGIFLALDPARIGAWLERPGVVERGSRLSRGFERWKAAHR
                     KPAMPFPGLPYVLLHSLSHLLLTAVSLDCGYATSSIRERIYVGDEAFGILLYTSSPDA
                     EGTLGGLVEVGRNLGRHLGRALDLARLCASDPVCAMHRPDSGHEERFLTGAACHGCLL
                     VPEPSCENRNDFLDRALVVPTVLGGDAAFFAGDLA"
     gene            18229..19020
                     /gene="drmC"
                     /locus_tag="PLP50_02185"
     CDS             18229..19020
                     /gene="drmC"
                     /locus_tag="PLP50_02185"
                     /inference="COORDINATES: protein motif:HMM:NF038319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system phospholipase D-like protein DrmC"
                     /protein_id="HPA50387.1"
                     /translation="MKGPWEELSRAGLEGLAGALAAGRISGPYSAGALGRHLPGVDLA
                     NALAELNRLHDAGLPPAHLAEVLRAVAAERKLREVTSDRVELVWTGPEMPGSQSRETA
                     IVARELFARAKRRLLVSSYAVFLGKDVFEPLARRMEEVPDLRVLLFLNVPAPGGADFD
                     PDAVAREFFREFLKKNWPAGRQPELYYDPRSLAAKADERAVIHAKCVVVDGHTALVTS
                     ANFTEAAHARNIEAGAVVHDSGFAGSLEAQFTSLVAAGKLRRVFP"
     gene            19079..20110
                     /locus_tag="PLP50_02190"
     CDS             19079..20110
                     /locus_tag="PLP50_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPA50388.1"
                     /translation="MSSVTRRAGASCIAGAVLLLTLTLADGAAAATLKDPRGAMGSKF
                     EITAVHADEAKGRAAIDAAYAEIERIEGLLSEWRESSKVSEINRQAGVAPVEVPKELL
                     HLVRRSLKVSALTDGAFDVTFHTVGRLWNFKSRTAPVPSEEAIRAALVDAGYRHVVLD
                     EGKSTVFLDRKGTRIGFGGIGKGYAANRAVFVLKEHGVTGGVVNAAGDLVVFGTQESG
                     KPWRIGIANPLEKGRVFAWLETTEQAVVTSGDYENYVELGGRRYSHILDPRTGWPVTE
                     LRSVTVVCPDGEVADALATGVSVLGVEKGLALVNRLNGVEAMLVDANGKLHFSRGLEA
                     MLTKEGGDR"
     gene            20125..20328
                     /locus_tag="PLP50_02195"
     CDS             20125..20328
                     /locus_tag="PLP50_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HPA50389.1"
                     /translation="MILALVGLSALFPGCTTVQPWERGALATEPMKLDDCRTHRFERA
                     AETYREGAVGGAGGKSGGGCGCS"
     gene            20319..21515
                     /locus_tag="PLP50_02200"
     CDS             20319..21515
                     /locus_tag="PLP50_02200"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HPA50390.1"
                     /translation="MQLSRLARLLGAAALGTVLAATAPAALAGEPPEGVLDADFLFGY
                     YDQDGDRSPVTGGVGTEKLHVYSPVLQLAWRLNESFSLSADVGVDQVSSASMGNIQSE
                     LSSASIPASDQRFFGTLRAKKKWKSGTWGLTVGAAKEYDYRSFSYGIDWGLELNKANT
                     ALSASVRRFDDAIDLIGIDGYGYQGPGLPITTGSGDRTTTDVTLGVSQTLGRRTVGSL
                     ELFLSSQDGVLSTPYHEVVLGPFEPGAVRVAERLPDSRLRKAVALRLSHSFSDRLVLR
                     SGYRFYTDDWGISAHAVELEPHFRLRSEREMWLFPILRFSTQAGADWFGTPGTFTGTE
                     TYYTSDGDLAETTTQKYGVGFTLNTKPGQTWLGIFRRFEARAAVYDRDDGLTAFSTSF
                     SFGWTY"
     gene            21515..21961
                     /locus_tag="PLP50_02205"
     CDS             21515..21961
                     /locus_tag="PLP50_02205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA50391.1"
                     /translation="MRRAALLLALLLAPPLAAAETDQHGVAQGWAGASSGYTVLDFAA
                     SWCTPCWSVLPRLEKLAAEHPEVRVVVVSVDDKVSGRDLLVSKLKLTIPVLWDGKHRI
                     AEHYRPEGMPHTVVLDPAGNVVHRHTGSGPKEWEAMVAFLAKATKR"
     gene            complement(21981..23246)
                     /locus_tag="PLP50_02210"
     CDS             complement(21981..23246)
                     /locus_tag="PLP50_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA50392.1"
                     /translation="MTHDAMALTDEKRLQDLRTLLEVAQAMTGVRQLQPLLELIVASA
                     SRLVDADRTSLFLLEPDGSLWTRVAQDSSDIRLPPGFGIAGIVARTGETISIPDAYED
                     PRFSRDIDLKTGFRTRSVLCVPLVTPSGSVVGVIEAMNKKGDAAFDADDAEALHALGS
                     HAALAVETQRLLEGELRRERMESELELARRIQEGLRPRVLPERPGLRLAAWQETAERT
                     GGDYYDVMEAPLGALDLLVCDVSGHGIASTVLMSTARAYLRALHLVEADPQRLLEKLN
                     RLMTPDIPDDAFATLVACRIQPAGEACYVSAGHLPPLVYRARSRSFDRLEETDLVLGW
                     STTTTYRAFDIEPLSPGDLVILATDGLYEASSPDGLLWGVDAVRDVIADAAHEGAHGI
                     IEALRAGVFGHAEKLFLEDDITLLVAEKV"
     gene            23290..25539
                     /locus_tag="PLP50_02215"
     CDS             23290..25539
                     /locus_tag="PLP50_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPA50393.1"
                     /translation="MKQILIRAREAGRSALLEHEVYEVLRAAGLDVPRFAFWEGEPAG
                     EAPAAVAALLSGGAAEVVLKIVSPEILHKSDVGGVAIVAAEPAAVAAAARKVWEDVGR
                     RMPDASRSGVLVVEKVKTKGGSPASETLLSLKQDPAFGPVLVLGLGGVLTEWFGAATG
                     GTSTVVLRPGRVREGLERAVATRPAAALLFKPSRLHDRAPLDLAATAERLERLGAVAT
                     AFGEGAQAGEADPLTLEELEVNPLLLAADGRWIAVDGKGSMGRSTATLPKRPLRKVKN
                     LLEPKSAAVLGASATAMNPGRIILRNLKLSEGVRYGHIWAVHPKEEAIDGIPCVKTVA
                     ELPQKVDVAVVSIPAEGARDAIRALCDTGKAESIILIPGGFAETGKKALEEEIVSILR
                     ASRSRADEGPVLVGGNCLGIVSKHQYNTFFLPQYKLPFHDAPGDRLLAVSQSGAYLVS
                     LTSNLDGIIFPKASISYGNQMDLTISDFLEAALEDDGVDVVACYVEGFKPLDGTRFVD
                     LARRLREKGKRVLAFKAGKTPLAAKATQSHTASLAGDYAVAEALMKGAGVVVAETLDM
                     LEDYTKAFTMLYDRLPRGNRVAVLSNAGFECSSVLDKLYGLVPAPLTDETKAKLEACL
                     PGVGHAGNPADATPMATTAQFVAAAQAMVDDPNVDLLLVSPVPVTPALDNLAPDLSGT
                     HRENIHSPGSLPNGLLELFGSTTKPVAVAVDSGRLYDDFVFVLQRGGIPVWRKIDRAS
                     RALSALATL"
     gene            complement(25622..27040)
                     /gene="glnA"
                     /locus_tag="PLP50_02220"
     CDS             complement(25622..27040)
                     /gene="glnA"
                     /locus_tag="PLP50_02220"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPA50394.1"
                     /translation="MFSDFSEAKSFVEKRSVEMVDLKFCDLWGRWHHVTIPASGFTPV
                     LMEKGVGFDGSSVGFKSVSSGDMALVPDLATGFLDPFWEVPTLSFICTTLEADTRALF
                     PYDPRSIARRAEEFLRASGVADESVWGPEFEFYVFDGVSYENGMNVASYRVQSSEGDW
                     NSHELGTGYNIPRHGGYHAIPPQDRLFNFRTRICKLVSQMGIEVKYHHHEVGGPGQCE
                     IEIPIFPMMKAADAVMIVKYVTRMAATQLGLTATFMPKPLYGEAGSGMHFHQRLLKGG
                     KNLFYDANGYGSSSAAERSYIAGLLQHGGAVMAFTNPSTNSYRRLVPGYEAPISAIFS
                     LGNRSAAIRIPKYANRPDNARFEFRPPDATCNPYLAIAAQLLAGVDGIRKGLDPTALG
                     FGPLDDDIFSWDAEKRATIKALPTSLEAAMEALEADRAFLLEGGVFNDDLLERWAKKK
                     RAEDREVRNRPHPYEIELYYGL"
     gene            27203..27586
                     /locus_tag="PLP50_02225"
     CDS             27203..27586
                     /locus_tag="PLP50_02225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPA50395.1"
                     /translation="MEVRFPGGVAVEAIHAGMTIRTDQPVAAGGAGSAPSPFDLFLVS
                     LSTCAGFYALRFCQERGLPTEGLGVTMDWERSPETKLISKIRISVKLPEGFPEKYRAA
                     ILRATDQCAVKKHLVSPPAIEVEAV"
     gene            complement(27597..28085)
                     /locus_tag="PLP50_02230"
     CDS             complement(27597..28085)
                     /locus_tag="PLP50_02230"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HPA50396.1"
                     /translation="MAIKLLITGGTFDKDYDELTGKLYFRDTHIHEMLKLGRCRLQTR
                     VRTVMMVDSLEMTESDRKIIEEICRKSEEEQIVITHGTDTMEETARVLGAVGLPKTIV
                     LTGAMIPYAFGSSDGLFNLGSALSFVQVLPHGVYVAMNGTYFPWNDVRKNRGLGVFET
                     LS"
     gene            28232..29095
                     /locus_tag="PLP50_02235"
     CDS             28232..29095
                     /locus_tag="PLP50_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50397.1"
                     /translation="MKVRLGTAILVGALGLAHGTVSAQGKPASSVKKFRFKAGKIETG
                     RLYAYDRFDPRGGRAGSVLVFVPDRKRVEVFRVKAGATDGQLFSGEMNWETFTLRTFE
                     LSKLSRDGTATRQATGTFGADSLAVSVEDPALYRGAEGATAFTVPLPAIPAHVYSLDL
                     ITLGLALRHVADPTGAVEFGVLTENPKVGPESPGLFVASGTATVEFVEDVTRDGVPCS
                     KYRVSGPALGGQEGFVWLHKDEGYLQDAEIPVSPTPDLPDVRITLRSVDKLSEKDWPA
                     RRLLELSGGPK"
     gene            complement(29114..30415)
                     /locus_tag="PLP50_02240"
     CDS             complement(29114..30415)
                     /locus_tag="PLP50_02240"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPA50398.1"
                     /translation="MTPTLRIGLLGCGAVGRELVRLFASEAPRLAEAHGVDLRLVAVA
                     SRALPSRSELLSTIPPGVRVGEDLSAVALAPDVDLVVELLGGLDPAGPLVESALRAGR
                     SVVTANKLLLATRGAELARLASRTGAALAFEAAVAGGIPILRALRESFVSDRIEEIGG
                     ILNGTCNYLLTEMARTGRPYAEVLAEARTLGYAEADPTADVEGADAACKVALLARMAF
                     GADVPLEVVRTEGVTRLRPVDFVYARLLGQVPRQLGLARRLPDGRLLLAVRTALVPAS
                     SLLARVDGPLNAVRVTTAHAGEYVLSGPGAGGAPTAAAVLADVVEIARRGAPFLVPPF
                     GTDAPRPCVAGGPADSVAPFYVRFVVDDRPGVLAALTAAFARAEVNVDAVLQVPWTEK
                     RALPFVVTLEPTPEDRVARALDEIASLPFLVEAPLLLPMVS"
     gene            complement(30412..31569)
                     /locus_tag="PLP50_02245"
     CDS             complement(30412..31569)
                     /locus_tag="PLP50_02245"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPA50399.1"
                     /translation="MIDRMQLGDVPAKPHVAFRSASGALRFEECFTRLGFDGEYSILY
                     HEGPPMTDRSIGPAEPGPLDRRVPAWAPDQPLRRRLLLGDRTPQGWTPVCGNADVVVS
                     LFDLPAAPAPETGFANGDGDDLLYVFSGKATLETPFGDLAAGAGDYVLVPRSVVHRWR
                     VDERITGIAFELRGGLHVPTQYRNTVGQLRMDAPFTHRDFRRPVFRGPRPGPTAVLVK
                     KGDAFVRREPLVSPFDVVGWDGTVWPFAFPILAFQPKTGLVHLPPTIHATFAARGALV
                     CSFVPRVTDFHPEAIPCPYPHSSVDCDELIYYVRGNFTSRRGVGPGAVSLHPAGIPHG
                     PHPGAYEASIGTRSTDELAVMIDTFLPLRVTEQARALELPDYHDSWLPKAP"
     gene            complement(31571..32734)
                     /gene="hppD"
                     /locus_tag="PLP50_02250"
     CDS             complement(31571..32734)
                     /gene="hppD"
                     /locus_tag="PLP50_02250"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HPA50400.1"
                     /translation="MSQELGIVGYDSFHFIVGDLERSRRFYTEALGFTEVARASRAKV
                     EAGGEEASVFGAGQIRVLVSTPVRPDSRAARWLRVHPDGVSSLAFRVRDVDAAWRFLE
                     PRGATFLTDVQVHEEGGGYFRTFSIATPLDDVTFRFIERRGTYPSFAPDFENVGDGLP
                     SDNPHGFATIDHVTSNAYTMLPVVNWYRDVLGFTRYWDVEFHTSEVDGGRKMTGSGLR
                     SIVMADPSSGIKFATNEPMRPFFRDSQINRFCEDHHGAGVQHVALLVKEILPAVESLR
                     SRGVQFLSAPPAYYDRLPERLERVKVSNVKEPMDALRANHVLVDGKDDRYMLQIFMHD
                     AGTLYGDAKAGPFFYEVIQREGDKGFGYGNFRALFESIEAAQSTDATSLEVVG"
     gene            32825..33748
                     /locus_tag="PLP50_02255"
     CDS             32825..33748
                     /locus_tag="PLP50_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPA50401.1"
                     /translation="MEATPTQTRPSEFFLRDLPLYLDYLAVEKGLARNSLEAYRRDLA
                     AFGRHLDAEGVPAAEVTRDVLVRWFGLRRSEGASPRSIARATSALRGLFRFLFAEKRI
                     AADPTSGLENPRRWMTLPKLLGRDEVERLLEAPDVETPKGLRNKAMFELLYACGVRVS
                     ELATLRIASLHLADGFVVVKGKGSKERVVPVADSSARWVARWVNGPRARARGAASPWL
                     FPGSGGKPVTRQTIFLAIKAAALKAGLDPAAVSPHVLRHSFATHLVDGEADLRAVQMM
                     LGHASIATTEIYTHVSRARARRVYDRSHPRA"
     gene            33741..34436
                     /locus_tag="PLP50_02260"
     CDS             33741..34436
                     /locus_tag="PLP50_02260"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPA50402.1"
                     /translation="MRRLLAAASLALLLPAMPAVSALVVLTEGRHLKAASFHLEGEER
                     IRIVLVGGGEVVLPIERVERIVDDELEPPAAVEEPVAPATKAWPSVRRPSGRPHSAPA
                     RWARLVEEAAEAHQVDPALVAAVIHVESSWKPRAVSPKGARGLMQLMPATARRLGVSR
                     SFDPAQNVGGGARYLSLLAKRFGENEPEKILAAYNAGEGAVEAYGGIPPYRETRAYVR
                     KVIALWTGEPAGS"
     gene            34462..35022
                     /locus_tag="PLP50_02265"
     CDS             34462..35022
                     /locus_tag="PLP50_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50403.1"
                     /translation="MFRTVRGFLMLAPPAFLAAMVAYLSAGPRAATSFTVYPFLADVL
                     GSGLPRSNVARFVVVTTLFFIVPYLVSGVLLLLADIGTSAAASLWRRERRTPAAGPRP
                     RLLAPEAFWTLVGASVLLAAAAGSQLPKLAHGGELPGGVNVAPVFVAAVPFVALAGAL
                     VLAAIASIPRAVARRFGPRQRSDGGL"
     gene            35031..36566
                     /gene="guaA"
                     /locus_tag="PLP50_02270"
     CDS             35031..36566
                     /gene="guaA"
                     /locus_tag="PLP50_02270"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPA50404.1"
                     /translation="MPSFDTVVVLDFGSQYTQVIARRIREARVRSIVLPYSATAAEIE
                     ALAPKGIVLSGGPSSVYDEKAPRGDEKVFDLGIPVLGLCYGMQLMALHFGGGVGHAPG
                     REYGRAVVDIVGGRLLGALGPRETVWMSHGDHVEAVPDGFTVTARTANAPVAAFEDAR
                     RALYGLQFHPEVHHTDHGAEMLEAFLYGVCGCAPTWTMASFRDSAVAKVRETVTEGVV
                     LGALSGGVDSTVAAVLIREAVGERFRGVFVDTGLLRKDEGRHVLAAFSHLGLPVTGID
                     ASERFLSALAGVTDPERKRKIIGGLFIDVFAENAKAVDGAAWLAQGTLYPDVIESVSI
                     HGPSAVIKTHHNVGGLPAKLGFRLVEPLRELFKDEVRRLGEELGIPRDMLYRHPFPGP
                     GLAVRIPGEITPERVRILQEADAIFMEELRASGDYDRTAQAFAVLLPVRSVGVMGDGR
                     TYEHVVALRAVTTIDYMTADWARLPHDLLDRASRRIVGEVRGVNRVVYDVTSKPPATI
                     EWE"
     gene            36758..37102
                     /locus_tag="PLP50_02275"
     CDS             36758..37102
                     /locus_tag="PLP50_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPA50405.1"
                     /translation="MEMKLYVGNLPYTMDDATLRGLFEGFGEVHSARVVSDRDSGQSK
                     GFGFVEMNDSEAQSAMSALNGQEHGGRTLRVNEARPQEARTGGGGGGRGYGGGGGYGG
                     GGYGGGGGRGRY"
     gene            37429..>49833
                     /locus_tag="PLP50_02280"
     CDS             37429..>49833
                     /locus_tag="PLP50_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPA50406.1"
                     /translation="MAFKRLFLTFAISLFTLLLARGASAQSGGALASGQLAIQGTRLS
                     IAAGDESQILDVAESARVRTCFAGVCGSMAPGDPRVNGLLVKAELSGPELQASATYTA
                     TPGGAFLLPGVQTEGTYVLSNIRLVRAAVPDGADEEVLGQATPAVVTLEVRRILATTA
                     TVKQLTLAELQARGIQITQQNYDAYSFAVGFAFQGGLVTIDFPVLFQDGGRVDLLSKP
                     EVKLDSLPEGLAAAVRRWQPPHIVPFKLEVGGPPGIFPREEMEELPPETKLFGVIVLP
                     GNVSFLHKYFDAQLLVANGAPAGSGAVLSNVTSTIRLPAAVPRELLRLAGTTPSVAPG
                     QAVPVLGLAGETQLGPGEQGTASFTLEGVAAGTHVVTMEVAADLERPGRDVLPLSGKL
                     KAAIEVVDARFNLAFSHPDVVRADEAYTLFVTVTNVSTRDWENAITLALDAAQATGAV
                     KADPNDTFQRQIETLGPGEGATVEFRLVAKVTGRCVATAFQSSGSVQGVIRLRTGVGE
                     HGIPLSPSSLVLPRFTGELPEPLVAANVPLLGLAYSLAVAPPGAIPPDLPRLVKSDVE
                     RRAIDLAEAGQRIFIGEELLPSLEALLLDQLGNRHDRPEFDQLRRTLDKGRTSAEGLA
                     SYLRQEQEARNLAASALLEHIADTMSYARPWLAATVESAGSGVAPTLEVRKLNGVTGV
                     TYLAYPAGDPRALRSLAFGETYDILDRPGGTRAPLSVVGRLSAEAGYSVVIHGPISGE
                     SMPVTLSFVAPSSSGGGFSHVRFPAFAVPPGQAWVVDVVRAEGDTRPLAFDLRHLATG
                     APVAGAPVAAVSAVERPPFRLIGARQDFVLDPYGSGVWYLFNRPPAKADAEAADRYEV
                     RTSFRGLDTTSSGTTLERSTVFSAASALLQPASERVVAVRFSQPVSAIRGLHDDQPVI
                     THEHEIDLAGLRDGWGDSLVGPVPDIAIEPDHVGGLVEGKVLRGTGEAVAGAVVRLVR
                     DRVIVMDGGADARVLHDLVAERTTDDSGGFFFPFIEEPILGVPRPAEKGAVQSGFRIQ
                     AVVPAGPDPLAPEEREEVTSIIRLQSRLARVNIAMLGRGTITGQVLYEDSSPVPAAAV
                     TAASTLFSEVRSTVSGADGTFRLEAMPVGPITVTARDAAGRSSYATVGLSGPGATADV
                     TLRLQRAEAPRTGTVSGRVLVRRETAAGPVTEPASGASVAVYSAGGPFGVATTGASGA
                     FLFDGVPAGRVTVQAADFSVSRTPALAVTDLAPDTTENVVLTLAVSSPRSVSGEVRYR
                     DPVTQIDGPVIGATVFVEGPGSFALTDETGTYRIDGVPAQGINEAPYTVHAIDFARKL
                     EGRTTVSVTETSPDPVVAATIQIKGTAVGSIDGVVLDPLGQPAAGVTVTLLPIGETTS
                     GADGTFSFEGVPARRYTVAAHRGDGLQPGAIGWIGEATTEVLFGGHRAFATIRLRGAG
                     TVRVRTLTANGPVRTPVSYKPTWFSPMELRIRQKATAIETTSDDNGSLELVVPVGELS
                     VGAASPFYGTVSYQGTIDFAGQVKEVDLVFQPLSTVKGTVVDVDGVTPVAGVTIELRA
                     HGLPPQPKGTGQNGEFEFQLVPRGPLSLTASGIVGTIERTGRTHAMVTGPGQELDVVV
                     RLKAKGTVRGGVVDETAPGVYSPVAWAQFALQENDFPFRRLPASPGYFKADANGRFAV
                     ADVTAGRFTVVARHPEQVTRQGRLSGEVNADFEVVEAGNIVLSGMVRDLTVLVRDPET
                     GSPVPDAQVTLSNGEAAVAGADGRASFVALPIRKWDVYAFHAPTGSGGRLDNIDLATA
                     GEALEVVVVLDQKGRVFGTLYDDAGKSTPVGGGTVRLEGTVNGRLWGSRVTALATTSL
                     EAESLGEFAFDGLPVGNYSFTAAVEGSDRRAEGAATLTPTEPEQSLALVLDAVREVYV
                     RVYQKVQAGVLVEVNPDPAQGDGVFAVSYGRGSSALAGSLIASRLLPDRPYPNHHYRF
                     NEVLANKSFWIQVEESSGERRRVLVRSTALSGGEFGAGTAADPYRIVLKARGTVRVTV
                     RDADGVAAASATVRVHAANGSWASAADSDGRAAFTAVEEGVVSVSASIPDSSSGASAS
                     RTLEWDDQVLDFDLTLSPVVSAHGVVYEAPENDSWDGDFSKLTPAEGVSVRIQPSAGD
                     AQLLITGSDGAYRFSGLAPGGYTIVAQSLDGSRIVRAAGTLGDDYGEDYLVPPLILDG
                     SRPRILTLSPPNGASSVSRTARVEILFSEPLLPAVLPSGMTSSHFHLTFGETPAAGTW
                     SSSLDDQGRQVVQFTPTPEYESSTTYSLRISGGLSGVQDREGRPLTDSGDIGASFTTS
                     DTVGPRFVRTVPSFARPVDPETPIRIDFGEVLVLTTEQLQAAVLFEWKNTAGAWTVHP
                     VEVSLTRGGYSILVKQPVGVTFTDDSLRRRLTVSGLTDALGNSMLPWSEEYRIYDENP
                     PILSIDPPPNAPSGVLIASVSYTLFPEFAALDDATPENEFGDIDRVEYTFASAADPEQ
                     PAPSPGAVLTAAPFAFGFVASYVGNGSDSRPFPVWLKAFDTSGNASAQVRLDMSVLPP
                     RSPVVSGVTVFATAPVAGTTYAGSTLTATAIGLSDDDDARLTISAELRRGTADSSELV
                     TSAPARSVDRPAEGWSALPAQSFTLTLPVTEAEGTAFIVRVRAVDSKGGVGEADAAFV
                     VADDALDPVVTNLVARRPNGDAATSFVIGETFVFEVTVRDGETGVKSLTVANPGGVLP
                     ATLAATRVETSDLFRTESVTVPASLTEAIQVEVRATAEDFGGNDAITTLSLDLAPSSD
                     PTDPAIEWLSPFEGGLWPANYSSVDPAKAGVDLLLRFRVTDKDVDGSGNEVPGSVASV
                     RLRGPVDATGALSSDWTSAAMLTQGEGEWIYEGVWRVPNGVAAGTVLRFEAEAVDAGA
                     NNTTKYVGVQAIAVRHVYEAATTAVGSGSEIPAPTGTEALPIFLLDGTTLSLYPRSAG
                     GPRSYANLYLYSGGALSAGAGSPVSVRRTTLTSPEVTSYESAVPYYPLELSIGETLAI
                     GHGAAIDVSGRGLLGGDGSHSPVVLSGERASLPGAAGSHGGLGRPPQGDWLQRLEDPG
                     SVYGSVREPRLPGGGGGNRGATGGGVVRIEAPSATVRLHGNLLADGGPVPGGGTVGAA
                     GGSIRLRAGTLEGRGEVRASGSFVAAGLYRPSGSGGRVSLSWVEPTITGITARADGAI
                     SSASSGPGSPGIPPLAGAGTVFLERLDSAGQPLDRGTLRTSNPSSTDLPAWTRLPGLG
                     SAVVLGVDATSKELVVSAPAAEGSIEGERIVVTIPALAGGNPTVLVLPVTAQTRTAGS
                     VGSADQSFRLAVAASEPEVAQIASAVSSGTAVTARARLSYAAFEARGAARVVSSDQLE
                     VGGVVDDRTAMSLDPSARILFGGEAPTVSFEGTSPAPGVNVTQGTAIDVRFRVADPLG
                     LSKLEETWLDAGTTATRTFSEPLEAFADPTPTRVTVPVSQPAGPVTYRVRAIDRAGRA
                     TESVATWNVVGDATDPVITSIALSPVKTEDVYAAGDKITIKAYATDDVKLARIRISVD
                     GHTIESATSPATLVWLAPPVTTSTAYDVSVVATDLTGNAATGGKVITVTPRIDAVSPI
                     VAIDCPSSGAILPSGYGNFVLRATASDDLGVHRVDAFREGETTPFATAYAASGSVKAF
                     DSSMPAITLPSVSEPTLVRFRVRAVDFGGNVSSEVQTEISVIPAVRITATDSITWSTL
                     VGETVYLESGTLVIDEPVALGGLLVLRGASVTHSAGGTKRLDLTVSGPLFVECGGSID
                     VTGKGYPARATYSGNVESTDSGGSHLGVGGVSGSAAKALTYGSVERPREAGSGAWGTN
                     PRSGGGVVRIGAGSIVLGGSMASILANGGLGGWEGGAGGSIWITAGTLTGDGLIEAAG
                     NDSAQDGGGGAVAIEYGSVSGTVLTRVNARGGGTGSSAENGGAGTVVLKGPGATYGTL
                     RVDNKGTSGQATVLPSLGSGVAQDGTSGATLVTQLTSIPAYFAGHWVEVRRGESLLGT
                     WRIKTITGGTVTLEPNEAEAIALQAGDGWQGVYRFDRVEVAGNAVVQSGDPIRTPTVE
                     LTGPTGTGGNTEAPGPVVADTVVVSGNVLTSGIEAKTLTVKAGARLSSPASTATEVKG
                     LELSISGVLTIEDGGSIDVTGKGYPARATYS"
     assembly_gap    49834..49933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            49945..53613
                     /locus_tag="PLP50_02285"
     CDS             49945..53613
                     /locus_tag="PLP50_02285"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPA50407.1"
                     /translation="MTLEPNEAEAIALQAGDAWQGVYRFDRVEVTGNAVLQSLDTVYD
                     GLPEITVSSPAPNATFASGATVPVSFQTADDRGVAKVILSLGESRVEILGSAQTSGVV
                     KAPWVGTTTTMPLEATVVDTVGQSTTRFVPLTILAAPSLTASISYPWDGARLWAGTTQ
                     EVSVQVPDARPIRSVTIGLGTFSMHFDSVVGPTLDVSIPIPPVAVDEDRLLTATVDDG
                     LGAPVTSPAVPVTLLVDNGPRVTAWTEAISPVNAGSDVQVDFSAFFRSWEGAQLRLQV
                     TGSVSLDETYDAGQFGSGGAGGVAALDVADSISWSFPSFQIPTDARGPMRIVVTAIDG
                     AGRMASADPIDLEVTSPLSISVVGSGTVSPGGTLELALSATGWNLPESVDLEIQGAFG
                     WSRPVSLGESYGESETVSRTIRIPVPRNALGEGAAAFEAYMDGGEGGYSRGALAAKLD
                     EGMASSNTVTFSVVDDVDPVVARVRSSEWGLVSGAEASFRADVSDNGTVQEVRFLVDD
                     VLIATVSEPTELSTYDSGPYLLPTAAEERSVVVRVEATDLAGRSSSAVRSFVLAPTGT
                     PLVRFRAPTSGAMAVAGQTLPLLAELRHSRLITQVAFYRDDDATPFATRSGEELTAEF
                     PVPPGATPGSEITLWVEATDDLGAVGEASAVVRIVAGALLTDGTILDADDASHEDETI
                     VVQGRVEVRGEHRFARLVVLPGAVLTHAATAGETIERLDLEVTGDVYVSPSATIDAIG
                     LGFEGGLQRSNPNPEGRTAPGLQEAQAGLGGSHAGEGEPAEDGWGAETYGSCRAPDLP
                     GGGGGASLSGDPGGSGGGVIKLHVGGRLIIDGAIDASGISGEGGAAGAGGSVQLEATS
                     IGGTGWVRAMGGDPGALVGRGGSAGGGRIAVLGPLEDAAPELSSASGPAWYASGGAGT
                     LLLRSPEDLHGHLIVDSGASSRVGWTYLAEVGWRWGDLAGEDDLVVRIDPFPVDVDLV
                     GRSIEITRDNALVGRFRVEESRPQEGSVKLDESAARLLPSWGFDIRGVQDFDTVSVKG
                     RAALDVSEVLESIVRLGPAAWMWTEGDYLCPGDPAPWLSTGTLSVEAWPGGNVVIDVE
                     SDDSNYCSGGEVPGAVVQYSVWIDGVEGSLLEVPIEGAPAEDARQLTYALPASLPLGE
                     HLLVVEVRDQSNQKTAIAFRIRVAEAPGLVQRLPAPSGGRSHPISLRPAKRFARPARE
                     AAGPVRTRGAGRGPQEPH"
     gene            53628..54473
                     /locus_tag="PLP50_02290"
     CDS             53628..54473
                     /locus_tag="PLP50_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50408.1"
                     /translation="MLPLAVLVVPVVAKAQPSEPFPAPFVIEHHLVDASLEGTPVTGE
                     TVRDTYGGSFLVSERPDGNRTIVDFARRELTEVRPTEGTYWSLSFGRFAELRKRLVDA
                     EKTETTPEVRTQAAREKRPVRIEEVPDGAEGKVRGALSATGSAGRRLRASVEGGSALD
                     VWLDVSAPRLTAAGVAALADFERNVLGAADGEVGPADLLEAVRLKEGGALAVRTRRGV
                     GRAEALRDDGPYLETVVTKAMPVAALPASLVSVPEGLRRVPSPLEVVVSWAEDEAALR
                     QRGRK"
     gene            54476..>71513
                     /locus_tag="PLP50_02295"
     CDS             54476..>71513
                     /locus_tag="PLP50_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPA50409.1"
                     /translation="MSRNRLAPLALATAVTLGVLPAHAQQDPFALPASFLTQAAFSCG
                     NVAVTGSAVVTSAGVGGAGHVVSNGNITLNGSAKIRGNATAGPGKTIKTTGSSQITGT
                     RSYLSEAWPCSPIDLAALGQTLATANDNALVPKTSSGKNPLSGSSPPDLTLNGSDTLV
                     LPAGTYYLGKITIGGSSVLSVSGDVRILATGKVSISGSSKVNNAGSPIAFRLFTSGSP
                     YALEGSARLKGFVYASSATAVVKVTGSGVFVGGLYGGQLTVEGSASLTRDVVYVPPAD
                     PLVLSITESGQPLAEGAVFGRAVTPVVSTTGGVAPVTVSATLDGAAWTSGTVVSANGE
                     HTLAVAVTDSSVPPTQLSETRHFTIDTEGPSLVIASPPPGAVVSTSPIVVTGTASGAT
                     SLTLLGAQIVLQEGGVFSATVPLVEGTNILRLVARDAAGNETIRDLPVVLDTLPPVVT
                     LSAPSSGTCLAAGTPLAVSGRYLDAHPRPAGAPEGPAVSLTLTLPSGAATTLPASVDE
                     SGAFTGSFPIPDGTEGSATLLAIATDALGSVSRVLSSVRFDSASPEVSMTSDGTPFPG
                     SGPGATPPQGATPAFVNRPLAARALVRDGAAAPPAATLTLDGQPYTEGTPIAAEGNHL
                     LVARATDCAGHESAAHAFFAVDTTAPALLATVPAENALLKEPVTTFSGTASADVVTVT
                     VGGQSVTPTPGADSTTFSLAPYAWREGDNTVVVELVDRAGNRASFTRAFQIKTTGPVV
                     EILLGGVPLGTGKTFFAPITPDIRTNEPLSGPGAATLTVTLDGAPYVPGTPIAVAGPH
                     TLKASVVDAAGTPAFDTASFTIDTSGGPSVSITSPVDGATLPGPTVDVSGTVSAAHAA
                     GRTIVRVNGREATVPDAGVTWTLPGLPLEPDAPNDIVAVAVDALGRSASAAVQVLVRS
                     DGPKLVLISPAEGTRTNRRKTDVVGAVVGGASATADGLVHAGALSTTIDATGSFRLLD
                     VPLADGPNPISVTAVDAQGRTGEAHVTVVSDTTAPTVSFTVSGQPLEEGATFAGPMTV
                     TVSVSDGAGDAPAPRILLNGAVVPSATETTEVAIPDEGGWVLSVVALDGAGNETRASR
                     SFIVGGGGCNLSDVRPLDGSTTPESKVTIVGRCGAALRVLVRVPQAGGGSPQEFVAAV
                     ADGTFAAGDVPLPVIGENALELVCEGAAGAPSTVAHRITRLSGDGPVVSIASPAPGAV
                     VTSSSATVSGTVSDATADLWVGGTKVGAAARTGTSFSQPAVALTEGPNVLLARAVDAA
                     GRSGEARVVLQRDSQAPRLTVVWPTSGARFGRRGDAPVVADVTGVVDLGSEPNLQSVV
                     VSSPADSITATVDPVTGAFQAPGLPLGDASGSVRVTVVATDSVGLTTTVTVDVDVDAG
                     TVAAGPALRLDAPVDLTRLTAASSPAIAVSGEAWAAEGATVSVNGVSLDPSALEWEPA
                     AADGRRHVTFQTTIAAPTTDGPFGVIVRVEDLQKRSASTRRLLVRDTVAPGVSEVVPA
                     SGATGVDSNGLVLVLFSEEVSRASLTATNGFVVTREGQPEPIVGTFSVAGSAVAFVPG
                     AALTPGATYRVVLGVGLSDLAGNPLEVPHESSFTVAPTLSGTLPVLDAPLPAVVCATA
                     LELKGSAPAGASLRVVVGAVTVNATADASGRFVLSVPLVANGYHDVSLRIVGRDGSLG
                     PALYGLVRKDCSAPFVERADLDREAGRVVIRFSERMAPATVTLSATAGDGASVVLSLE
                     EEPTVPRPGTLVLDADGRSVRIDLPSDADAWWREKAVRLRVQEPAADENGIPLSAPWV
                     TTFLPGGGTGDLTGGFLSGEVYDDATGRPLDGADVRLYPSSAAIPGAVAAPDVALEAT
                     ATDARGRYGFFGDVAAGRYAIHLGKAGYVPALRRLPLAPATGAVPFDARLTPLSPLAA
                     QRLDPVVGGSFEGPSGLLLEVAVPAFVDVPQETLGVRLTPLTAQALPELLPLGWSPLA
                     AADVTLELEGTTPVPLSQSAAFAAGAVHLTLPLPANVPPAAALVAVRHDVASGRWLTL
                     GPVDRHAGAGSTDVARVPLVAPGAVAVVLADVEPAIAPPALSGAEGEPLTASALPSPV
                     PPLTATLTLDPAVVSPTERATARVVARSADGTTFWPSGLAVQAYLDEKLVLSGGGELY
                     EAPFTADLLLTHHPLSPEEESGATAGTVGALSFAVSPSPKAAEVLLESGWENVRLYPF
                     PESLERGSILGALGGTVTSPDGVELTLPEGALAESVAVEAKLLTAAELSALPAPAGFD
                     LLAAVRVSLSGRALARAATLSVPLPADSPDDPAAEARLLLAQLVEQPADGRGAFARLT
                     ARATRAAGPRALAAPEAAGSPLPLEGLLSEGTYLVLRAQAPLGFATGFVMVGQGNGLA
                     TSRVTTPTLGTADLSRPGGRYAIPVPAGDNRDVFALHPQLDEAATGRIPSLAPSAVVA
                     LDLLVKPVGPHVTSVQPGDNATSQAIGSSLVVHFSEPIDPASVVPGLLTAELLGDDGA
                     ATGAFFNGTLTLQPDGTTLVFQPTHPLPPGRRIRGRLTSGVRDVGGTPYEGALPYLWS
                     FTTSTEVTSGGQIDLTKIRLLLPENGTARIVGTAGAIPTGWSVSASVENAKPSPACPE
                     TTTNVDTTGAFSIVAGCASTPVSLSSRVFLRALDSAGNLTVLPLGPYVTADGLGFVAQ
                     PGEKAVYTTPEGVEVTVPAGAFDEAKVVRVAKKDPATLGFDLPTYLELGAFVDVDFDG
                     EASETLRLRIPVTTTAPVGTLVFAGSPIDLPWGRKLRLIDLARIVDGGGGAKVISNAE
                     ADQPVDPTTGGSGLIALEGETHLASQLPASVIRMTLLEFASRGSAAFCFGVGAELTAV
                     TGSTVPNELMTQVGVLWNEMADALVFVPPAYNWTGRFILPAVVGQAFTIVAKDRATGW
                     VINRHEFGPVSPVPGALVPVTDFPDNDVTIPRLIDASPFGLTRFSAFTADGTEPTCLS
                     IRLELRACATSDGFLRIEADPAYPLPDESRVSLQSLASPAGDLSARRVLNGSLGSEPL
                     QLAPKSGEDLLVTVTPGDLNPDGLEVLRFVFDKPLAPVPAPETAAHLVDCGPLPGDCR
                     SAVPFPVELDAPGGIHAPFSEALVRLRGTLPRGHLFRLTLQRDQFRRATSGSDAVYSG
                     PSDFLFATRKAAEEPLATSDALALGDTNLARDLVKLGNLLFVATGTGRLLAFDVSTAR
                     SGDVAGGAPSPFELFARMTDSFDEIRGLATDGHNRIFLNVRAGNTWGVKVVRLEDVRG
                     AGDCPDGDSPSWAEGVPCFSPVEGSVRTAFLAEGAMLGSEYLARVGSLAVGRPVGLEL
                     LAKDDGTKPTDLKSFYEENKPPGDPAFDELPRDAQGFVTFGLLLSTVDHHAARSTRTC
                     SGEAIDDRYQRVTVDNVTTGQSWSFDVENAWPGTSPPSPGVRIVSGLKARRGDEFRLR
                     VNVGLTGYVPVLGSGVTVVDLNRFYRLPASGSPDFEGQCGRRLGRYEEAGSFGVAPCS
                     AGKTPDLSNTYAAAVLGQTGDEAEPRGRDTRSTFLVVNHYGGVEAESSTSDLGDLQTV
                     QGVCLTDFDTDPSLPVSLRSVAVATNVRWRDEGLVFAGGRFVAKGLGIESAERGGDLV
                     FYSLGPEGIVAFDATNRRLGAPIGLYYRKDHWVTRIQADARGGRLYAGGFHAITNEPF
                     IDVWDLYRVNGGPDEEGRDARLLLSLKAAWDTNHIAFDEAGTGFLYTWGSAVAGEAAR
                     GFILPIEDARFVFAGLYSPEAAEPPSDPPAVGPLPTLRPTSAFVPLGVPLRVDSVAEC
                     DPANQAEDEKVATAAFRVRIALPGDLGETLVAKVQSLRGLPAEGQLGAEDVGAFVAPT
                     GGPGWPAREVFITLRRLGTADASAGNGAYPGEGGRLSTAYNLYESKEVVLLLADPRAR
                     ATYTLQKLEGEATADEESQCRRCTRPAYLQRLIDDGLLQADDILELLAAGPYVRATLS
                     TERTDPLDTSVAVPADSGATAQARVFFQGNSAGYRAPWGLARATSWADSVPAPIQVSL
                     EEPVLSPAYWDSGERGVGVLLGGGEAVFTATDYAKPGRGIDVAMERTYRSGVLGFGPL
                     GSAGWGSPLLQHLRQIPLYGDGKRPSGEGPSSLDQLTGIPEMVEYYDGTGHVFRFISK
                     GNGGCPTWAEEDSLGSYCVPKGLYLRLRKLEGGKRWQLLGRQQSVLLFDEHGRLLEVR
                     DRHRELATDPHVRGNTHQLVYDAFGALAQLEDDYGRILRFEEFSNPREDGEQYGLLKS
                     FTDFAGRKTRYIWRSQPGTRDDKLLRKAILPSVTSSLGADYSHADPTVSYSYGETILA
                     PSAPHHGPDFSRLRLTGFRLPGTDESAPSRVGLDYDASSGRVVGVTVPDGGRWEIQKA
                     EGPPAAPATSARVVAPWGHALDYTLESGRTATTLEGVPALLAGIPVTGPDASPADHYA
                     LTIFGYEEDGRLGKVTIPGGLERSLSYESSAGDRLRNANVVTVVEKGFARQGDAGCSI
                     GALSEVTRTTTLEYDPANAPHKVKTPRDVLLTLAIATSASGAVPVSVPFGISAATGIR
                     ELEASSSFDSHARNVRTAGPEGEQGTPVVEVTDFHDDARGRPQKGYLKTVVRGPIVES
                     YERYDAAGNPQTRAFGGVVETSRFDEWDRPVQIEAGGSDGTYSPTNARIQRGFDAAGR
                     LVLERRRQTPLGDVDTITAYDSADRVTSVTINRVADASGDATATATTRFDYDAYGRLW
                     KVTSPAGVVAIYDYDTAGRISSETTGSGARRRLYDERGRLVYTTDGHEGYWRGLYDDF
                     GGLTEERIATGAVTRRCFNAEGRPTREEVRQADGKVLSDVSREYTSFGAVSTLVERLS
                     SGSPTPEQRVTRMSYDRSGRLLTVKTGDGDTLVRDEATLVYDSANRTVQRTDAAGNKV
                     DTVFEPASRLFPSSVRLTESGRSPYSTDFLLTDAAGNVLFQRGSDGSTVLRAFDEAGR
                     VTSEVMGANPPATYSWDGAGRLLASTLPAGGQTTYRYDLDGRVTSETVTSSTGAWITS
                     HGYDVAHSGRLTGVTFPDGTAEVYTQFDPDDVPRQWTNRHGQVVSSVVDPANRLRGLT
                     PSGGPANLATYSLSWDYDDLSRLTRAEKTGVPASAVVNSYDLGGRPETTTLGIGATFT
                     RSHDVWGQPFRSAITSEMSGDAAAGVAVLRRDHDALSRPTRSSLDQDGGPDGSGIPGA
                     RLSWSGASNLELIATLGPRSFQQTRSYDSPGRTTSFGFVGSRAWGDVGVAYRPDDSYK
                     TGRVAPSAGLFSGQGWSFTPDVRLRLTSAESSRETFHLSYGAGDELLGHTRDKQSLLA
                     SFSPGPGGRLLSRNGDAFVYNASGDRTEDDRFVYGWSWRGELVSLEVKGVWPADGPGE
                     EPRVSPWAGHKLFFDYDALGRLTARTHLGVLPSPVDPDTSRPFISRREVLWDGGTLLA
                     EVEKDAQGAIRWRKTLVPGATGLDDAVQMRVEDYGAGNTPARRLYSFVRDEQGTVLGL
                     VDEQAPEPARGPPLPARYLYTPLGEAHAETGPELLRARFDATRTSLNGASQNPPRPDE
                     TVGGAVLFDLSLTLDPETLGQGVPIETWSSADNAWQRVPAGRFLLALDQDRPTSISVL
                     PLDGWPKETRYRIRLTSDLLDDLERPFRAPDGGVSFDVLLEMPADGLTHPVYDRTIPT
                     TFDTARAASDTVGGRVPGGMNLLHAGAWTDPVSGLQYLRARWYDPRTGTFLSEDPAGD
                     VDSPNRYAY"
BASE COUNT        10495 a        24886 c        25229 g        10803 t
ORIGIN      
        1 gccgctattc atgcggggca tccccgtcga accgagctgt cttggacagc agtggcagca
       61 ggcagtcttc gacaacggca agaccctcgt catttccgag acgacgcagg gacggctcga
      121 ctcgctgact ctcatccgcg tcggccgcgt cgcgatgctc tggaaccatg cgcgccacgt
      181 gatcgtctac gagcggacga cccgcacggc gccgcgctac acgcatggcg agcagcccga
      241 cgacttcgag ggcctcgcgg cgctccgaaa ggtgcgcgag tacgtcgaga ttctccagcc
      301 acgccgggac tatcccgact tcccggccgg gctcccgtac gcaggcccgc tccagcggag
      361 caccttcgag acgaccgtga tccccgtgaa gtcgacctgg ggacgagacg tggagaacgg
      421 ctgggtgatg cctctgcgcg ggccactgtc gcccgaggag gaagaagcgt tccccgttcc
      481 caaggtcttc tccgagctgg cccgggccgc ggggaagggc gaagggcgga tcgcccaccg
      541 cgtgacgaac cccgacgtcc tccacttctt cacctccacg cgcgacgagg acggcggcga
      601 caccgacgcc tggccttcct tcccggatgt ggacttcccg gtgacagaga ggccgtccca
      661 gccgccgctt ccgtccgcgc cacgcttctc ggatctctcc atccagcccg acgcggtcgg
      721 cgtcgggtac gggcagcggc gtttcaccgt ggatctcgcg cccgcggagg aagccgcgaa
      781 cctgatgcac ggccggcccg tccccgggat cgaagctcgc gtccacaacc tctccctcgc
      841 gcgtggaaag cccccggtcc tcgcgccgtg cgactccctc gaggcgcttg ctgggcgccc
      901 gttcgccgag gctgaagctg tcctgcgaga cgggatcgcc gaactctctg gctcgctcgc
      961 gcggctcgac cggggcggcg atgcgacgct cggccagatc gggcaatcgt ggagtgaggc
     1021 gcaggcgctg cacaagaacc tggcgaaccg cgccacggcg ctgaggggaa cgctcgagaa
     1081 acagcgaagc gacctcgaga agtacgcctt ctcctggagg aaggagcagg acctctggct
     1141 cgagcgccac ggagcgtcgg cgaagcggct cgtcgagcga gagctgaagc ccgccgtcga
     1201 gacggccctt caaaccgccg gggaccttgt cggaaaaggg caagacgccc tcaagcagtc
     1261 gctcaccatc gacctcgaag gcctctgccg ccaggcgcgc gggcacctcg acgaggtctc
     1321 attgctgccc gagctcgcgc tggagccctt gcgccgcgcc tcgcgagacc tccgcgcgcg
     1381 cacggaggcg accctcgccg gcgctcgagt ccgcatcgac gcgctcctgt ccgatctcga
     1441 ggcccgctac gcagcggagg gagcccgagc cctggaggcc gagctcttcg aagggctctt
     1501 cgacgtccga gagcgtctcg tcgctttcca gtcgcaggcg cgcaggttag gcagcgaggc
     1561 gctcggagtg tggttcgggc tccagcgaga cgcggccggc aagccgaacg cggtggacca
     1621 gatcgcggac gcggtcaaca tcgcctgcgg gaatgtcctc cgcgagctgg agctcgccat
     1681 cggggagatc ccgccgctcg aggtcggcgt cgactgggcg tcgattcggg cgctcttcgg
     1741 gagcctcgag gtcgacgtcg caccgctctt cgacaagatc gacgcgcaca tctcaaagct
     1801 cgcgaacgtc gacatcgaga tcggcggaaa gcgctacggc ctccttccga aggatgggcg
     1861 gtcgcgctgg aaggagctcc tcgacccggg cgcatccatc gagaaggcgt gtggcgccgc
     1921 gcacgacatc atcgagaagc tcaagttcga gaacgtcgcg agcgacctcg cgaccgcgca
     1981 cggcgtgctt cagagtttcg tcgagaaggc cggcgagaag ctcgcgacat cgagccccga
     2041 gctcggggcg ttcctcgacg cctttgggaa gatcgaatcg tggaactcca ccctcggcga
     2101 gacgggcgac atcaatcagc cgctcgacgc gatcgagtcg gcgctggaag agctggggag
     2161 cgtcctcgcg agtgccggct ctccggtcgc ggacgcggcc gccgcggcgg gtcgcgccgc
     2221 caccctgctt ggcgagcacc tggatgccct cggccggcgg gtggaggggg ccgtccttgc
     2281 cgaagcccgg gctctcggcc gactcctgcc gagcgacggg attcacctcg agctgacgcg
     2341 ggcgctggcc gaaggcccag tgacggacgc gctgcgctgc acgcggcggc tcctcggcta
     2401 cacctatgac gcggcaagcg cgctgctcca ggctacgcgc agcagcgccc ttttcaacga
     2461 cctcgggccg ggggtcctca actcgctcgg cgtgcgactc ccgttcgatc gcctcggcga
     2521 ccggctgctg ccgcgcctcg atggtctggc catcggagac ctctttcccg acttcggcgg
     2581 cctcaagctg gctcatttcc tgaagggact ccgtgtgccg tccgacccag cgcaggagtt
     2641 cggctgggtg cgcgtgacgc acggcttcga caaggaccgc ctgcttggat gggccgaggt
     2701 cgtcgtcgac aagcggttcg aggaggagcc ttcgcttttc gacctgccac cgttctcgct
     2761 acgactggtt cgacctcact tctcggcaac gagccgattc gaggccgatg ccacgggcgg
     2821 ccgccatcag accagccggg cctccctggc ggccgactgg aagctctgcc tctcgaacca
     2881 gcccgtgatc accatcaaga agggcgtgct ccggcacgag agcgacggag gcttcgggtt
     2941 cgacttcaag gcaaaggacc tcgagctcgc cgagccgctc cgtttcgtga ccgatgcggt
     3001 gagcgcgctc ctgccgcagg cggaggggct gaccgtctcg ccggtccctc ccgggggcat
     3061 cagcgcagag ctcaacctcc cgctgccgga cctcgccgcg ggtgccttca cgctcacggg
     3121 catcaccctc cacagccact tcgacctcct catcgggcag ggcttcgagg tgcgggcggg
     3181 gctctggctc agcaagcccg atcgcccctt cggcctcgcg gtcctgtttc tcggcggcgg
     3241 cggctggttc ggcgtcgacg tctcctatag gccgcccgac gtcttcgaga ctcgcctctc
     3301 cgtcggaatc tccgcgggtg ccttcgtttc tctgaacttc ggggtcgcga aaggatcggc
     3361 cggcgtcctc ttcacggcgg gactcgattt ctacagaagc gaccgaaccg gagcccgggg
     3421 cgacacgtcg gtttccctcg ggctcctcgt ctggggagag ttctcgatcg tcgggatcgc
     3481 ctcggcccac ctgcgaatgg tccttcgcgt cacctaccgg aacggggaga tgaaaggcca
     3541 cggccggatg agcgtgcgca tcaagatctg ttggtgcttt acattggagg tggagtccga
     3601 aatcgactta cccttcgata gggggggcgc gccacgggca atgcccgcgg gcacgaaggc
     3661 tctctcggcg cctcgactcg gcaccgccgc gtcagtcact cccgtcgcga cgatcgaacg
     3721 agctgtcgat ctccacctcg gcagcctcga cctcgtggag gccgcatgag cacgctgcgc
     3781 gtcatcctga gtctccacgc cgtcgggcca cgcgaccatc tcctcgatct cgacacgatt
     3841 cccttcgccc ttcagctctc cgccggattc gaaggcgacg tgccggatgc ggcgaggaag
     3901 gcgtgggcaa ccgccgcgct tccctgggag ttctgcctgc ccctgcctgg aggcgacgac
     3961 ggaaggcgcg accagctcga gctgcgcctc taccggggcg tcacgaacga cctgaacacc
     4021 atcggcgtcg tgcgggcgca ccgactcggg tacggcccgc ggcacccgtt tctttccgcg
     4081 ctcgcgcagg gcgtcgtcga tcgattcgtg aacgaccaga tggtcctgca gaggctcgcg
     4141 ctctcgtggg acgcaaacaa gccggttgcg gcctccgaga cgagtccgca ctggggtgcg
     4201 atcatccagt ctctatccgt gctcccgccc gagattgcga gcggcgtctc cgcggcctgg
     4261 tatttcgagg ttccccggga cgctctcttc aagaacgtcg gcgggacctt cacccccgtc
     4321 gttccgtact tcatcgccgc gcccgttcgg ttgcgtgatg ggagacacga ccgggaggcg
     4381 tcccttcagg ggaagcggga gggcccgtca gaggtcacct tcgaatacga ggacgtgaaa
     4441 gctctcccgg cgattcgcgc catcggcccc gcctttcccc tcgcatcgct cggggcggag
     4501 gcgggcggcg gctccttcag ccttggcgac tactggcttt cggtaaagga ctctcgcggc
     4561 gtggccctcg cggacgtgga cggcgtcctc gctcaggcgc tcgaaccacg agtgatggcg
     4621 gcggaggtcg accaggacgt cgtcgggagg gtgctacgga tccgcgacgg cggagcgccg
     4681 cggcgcgcga ccggcgagca ggccgagctt ttccggacgc tggccggcca gacgagcttg
     4741 gaggacgcga tcgagcgcga tctgatggcc gagagcgtcc tcgccggtct gaagaacgcc
     4801 ggaaccgacg acgcccggac tgtcttgcgt cgccttctcg gggatgcgct ctcgaagcgc
     4861 tggaaggatc tggcgggttc gacggtcgcc gccgcccgcg agcgggggct ccttcgggat
     4921 ggcgaaatgt gggagctcga cgatgccggg gcggacgagc tcgcgaggcc gctggtcgag
     4981 cacgccgtgg cgcgaaacga cacggctgtc gtccgcaccg cactgccggg ggaggggctc
     5041 gacttcgtgg ccggtgcgag cgagcatcgg ttggtccacg aggaaaacag tagcttcggg
     5101 agcaaccgcg aaggggcgta ctcggtcgta tcgggtgtcg gcgtcctcgt gcggcggtgg
     5161 aatgacaagg cgaccgccga atcacgcccg tggcgtctcg tgacggcggg cgtcccccac
     5221 cttgcgagca ggggtgagtg gttcgagctc gagccgctcc cgctcaccgc ggggttcctg
     5281 aacggcgttc ttcggagtga caggacgtac ctgggcgagc ccatgcggac gccgagtacc
     5341 ctggtgaagg cgcacgccta ccgggcgccc gaaaacgaaa agcgtcccgg tgacccgacg
     5401 gccctcgagg acctcgagtt cctctcgatc gcacactaca gagcggccaa cgaagctgag
     5461 gcgccgaacg ccggctgggc ctccgccccc ccgctccggt acggagacgg atacgacgtg
     5521 gcggccttcg tcgtggacaa ggccggcggt cttcccgtgg agatggcgcg gtggcgggaa
     5581 cagccggtga ccgtcgactg gtccagtctt cgcacgctcg cgccacgggg attcgcgcgg
     5641 attaccttcc tgcgtcgcgt gccggtgggg gccgtgaacg tcctcccggg cgcgaaggaa
     5701 gacgcgatgg aacgagccgc atggcccgcg attcccgatg gggtcgccct gcgttcgcgg
     5761 gagtggtacc gcggcgcgaa cctgggtagc ggcgactctc cggcccttct cttgagcacc
     5821 catccagact ggagaggaac ccttcgggac tattcattcc gcgtcgagcc gccccggctc
     5881 gacgagcaca cccttctgcg ctgggccgtt ccggcactcg gctcgaagga cgccgtgacg
     5941 gagacgaaac gtcttcgaga cgcgtgggtg aggatcctcg cgtggcggga cgccgagacg
     6001 gcgcgggacc cggaagggaa gaccccgcgc tactcggtgc caggcctgcc gcacgacccc
     6061 gccgtcacag cgctcgggtt ccgactcacg aaagtcgacg cgtcaggtgc gggcgcagag
     6121 ccgaagctgg tggtcgttcc gctcgaggag cgcgcgccgt tcgagatgca gccgatccag
     6181 gtgaccgttg ccgcctcgga gacaacctcc atttcctcgg cgaacgcctc ggtgacggta
     6241 accctgaaag agggggattt cgccgctttc gaggtgtgcg ctctcgtact cgagacggac
     6301 ttctcgcggt tcgagggtga agccatgaaa gaactcgtag aggcgcgcct ttggcctcat
     6361 gccggaaccg cctacagggc cttctgcggg acgactctac tcctcgaggg cgcgacgaac
     6421 gcgctcccgg aggcggagga cctgttcggg gcgcttggac tccaggagcg cgaggggcgg
     6481 attcacgtca cgctgcaggg tcgtcccggc gatctccact tcgtcgatac tgtcgagatc
     6541 gagcgggagc gctgggtctg gcgcaaccgg cccctggtga tgagcgccag tagtgacgaa
     6601 ggccccgagg agcgccgccg ccgcctcgcg agcggcttgc cgaacgctct gtccgacgcc
     6661 gcgatgcgcg acgtggatgg tcgcgtgcgc gagttcgatg ccatcgccgg gcccgatcgc
     6721 ggctttgtcg accgcggcac ggtccgcctt cgcctgccgc gtgacggcga tggcggcggc
     6781 gcgccgaatg cgcttctcct cgtggacgaa cgagacggca gcccgagcgg cgattacctg
     6841 agctacggcc tccgggtgag atcgcgctat gcgcccgtcc tcccgccgaa tgagcgtgag
     6901 gttctggcgg tcagggcggc gtcagagtcc ccggcgaacg agcccgacga cttcacacgg
     6961 aggtggagga cccgccggcg tgtggtcggg acctaccgcg gaggcccgtt gaagcccccc
     7021 aagatcctgg cggtcgtccc gcttacacgc gatctgacgg atgaaccggg gcgtgacaca
     7081 cacacaccgg gagttccgcc tcacctcgtg ctcgtcgacg aggtctggtt ccgcgagtac
     7141 gggccgggcg agcgtctgga ggcccgtctc gttctggaga cacgggagat cggcgaggcc
     7201 gaagacggcg cacggcccta ccgcaccggc ccgatgccgg atcatcacct ggctccgccc
     7261 gcgggaacgg agaccaagga gacgggaacc gaggtgtcgg agccgaatcg gtactaccgg
     7321 caccgcatga gaagcgacac cggcgacgaa gaagatccca tcctcctcga cgtcttcggc
     7381 ccattcggct acacggtcga ccggtcgggc aacgaggcgc tcgcgaacgc caccgccttc
     7441 ctcgtctatc tgccgaacga tgtggggccg cactggtgtt cttacgtccg gttcagccgc
     7501 gttctcgacg gcccgggctg ccccgagccc aggcggagcg agaggtcggg tctctacccg
     7561 ctctacacga ggccggatgc cggccggctt gcctcgggtg acgaaggcgg cttgcttctc
     7621 gcgggaggta tcgcccgtct caccggcgga ctgaagctgc tgctcgaccc catagcggtg
     7681 ccctctggca agcccgcggc ggcgtccgag acgctggcca ggaggaacta ccggtacctt
     7741 ctcctggtca gccgagcggt cgtcgacgcg gcgcagggcg tggaggtcga gcttccgcag
     7801 aacctctacc ggctcaccgg gacgaagctc aacaaggacc gcgtcgagtt ccgtgagggg
     7861 cagccgctca tggcaacccc gatcggcgac caagcgaggc caggggagta tcgagggagg
     7921 atcctggagg tcctcgtgaa cggcgtctac gaagacggtc gcgctccgct cgacgacgct
     7981 aagaacgtca aggactttct gcgccggctc ctgcccacgc cgaaagagcc gcgcgaaggt
     8041 gatcgccttc cgtacgtatc cgagctcgag gacgcgcccg gcctgctctt gcccatatcc
     8101 tgcgccttcc ccgttcgagt cgaggacgaa ggccccccgg aggccctcga tccgaaggtc
     8161 tacatgacgc gaccgccgaa gcccacgcca aacgacatgg tcaccctccg cttcagagcc
     8221 gtctcgacag gcgcgaagct cgagtacgga ccgcacgccg cgctgcccgg attcacccag
     8281 gccttcgagg tgagagtgga ggacaggcgg gagctgccgt cgacagtgca cctggaagga
     8341 tcggaggtca ccccgctccg gtgggcatgt gggagtctca tctggcggac gttcgaagac
     8401 gtgaccagcg agggcttcct cgagtacggt ctcgatctct ggaaggagac gcaagtccgc
     8461 gaattccccg acgacgagct ccgctggacg atgcctcatc ggatccccgc gtcgatcctc
     8521 ccgggagaga gctggacgca ggaggtcgag gagctcgtga gggaccgtga cgggaatccg
     8581 aagggcccgc ggaggtacac gcgcaccgtc accttcgagg gcttcgaggc acttgtacct
     8641 tccggagaag ccttcaatga gtgcgcacgc gtgcgcgagg acttcctcgc gccgggcgac
     8701 gggggggccg aatggattgt ccgctggttc gcgccaggga tcggcgaggt ccgctccgag
     8761 gactcgggag gaagcgtctg ggagctgttg gcgacgaagg ctgctgatcc cgaggcggag
     8821 aagtcagtgg ggccaccacc ggaccgcccg gcgttggttg gccgctcatt accgctctca
     8881 ctgaagaacg gagagtcaac catgacgaag aagagtctct cgaggaaggc gacgcccaga
     8941 agagggagcg gtgtcatctc gacgaagacc ggcaagccga cctcgctgag gggcacgagg
     9001 gcgccgtccg accggaaggt cgtccggaag acggcggcga ggatccggcg tcgcgtgagc
     9061 ctgaccgcag gattggaatc ggcgtcgctg gcactcgcgg ccgccaacgt tccaaccgca
     9121 agcaagaagc cgaacgagcg cgtcaatgcg ttctgggcga tcgcggaggc ggtcgcgcaa
     9181 gagctcgatc aaccgtcggt tcggtaccgg gcgaaggtct tcctcctccg ggtggcatgg
     9241 cacgagtccg ctcggctcac gacgcggctc cagggaggag gcggacccgc gcggggtctc
     9301 ctccagttcg agaccatccg tgccgaggaa gctgggggct acgccgagca gaagggctac
     9361 ctgaaggacc tgtcgagagt gtcatctcga accgaggccg cactgaaggg ggcgtttgcg
     9421 gcgttgcatg ggcacgcgac ctatccctcc ggcaacctga tcgaggatct cctctccggc
     9481 aatgcgaacg ccaatgatca cttctgcgtg ctgctcggca gaatcgctct caagcgaggc
     9541 gcaaaggccc tcccagacga ggccgatctc ggggcgactg cggattactg gtattgccag
     9601 tggaatcaca acttcagcga caaggtgtgc caggcaagca agaatccggg tgatccaaac
     9661 aacgcggacc ggaacgacaa gatacaggcc ttcttgtcga gctgcgccgc gttggcggag
     9721 catcttccct cttcgtagtc tcgaccctca gaggttgtgg agctatcccg gacggatacc
     9781 gggacagcgt cacagcctct gagcggatat ggggagagag cccgggcgga ggaatcggcg
     9841 gaggatgctg aaggacgaga ctctggagtt cgacacccag caggaggcgt accgactgca
     9901 gagacgttca ccgtgggcaa tcagttgtgc gacgttgagc gtgaatcctt gtaggcgcgc
     9961 gcgccgtctc agccgacgcg cgacgcccct gccgacgaga cggtcgccga gcatggcgcc
    10021 gacccttcag agccgctcat cgcgcgacgg tagggttggc tcttcgaggc ggtccggctc
    10081 gggctccggc aggagtacgc ggagcacgcc gatgtcctct ccgttcgccg cagggcggga
    10141 agctcgacgc agcgcagcct tgcttcgaag gtcgcaggct gtcagagtcc cgccgcatga
    10201 gcagcctgat tccgaccgtc gccgccatcg ccgcgctgat ggccccgcaa ttcggtggca
    10261 tggacctggc cgcggcgcag aagtgggaag cggccaaggt cgtccactac cgcatcgttg
    10321 gcgtgcacga cgggcgcgag atggtcgtct tcggcgacta cgaggggaag gcggacgtcc
    10381 tcgatcgcgt cacggtcgag ctggactggg acgtccggaa gaggaaggtc atcggagcgg
    10441 tcaaggtgac ggacgcggcg tccacgctga agaacgtcaa gtccgacggc accaactgcc
    10501 cgccgccgca gctcaacggc gcgtacgagc acttcaagta cgtgtcgcac tcgatgtcga
    10561 cgaacgacca gatcgtcatc aaggggtcgc ggacctaccc ggcggcgaag gtctccaact
    10621 acccggcgag ctgctccatg cggtccattt ccggcgggag ggaggacacg atcctcgggg
    10681 tggcgatcgt cgctccggag gtcctcggca tgccgatcat gaaggacggc cccgtcgtcg
    10741 tcgcggacga ccggaagacc ttctccgtca aggcgaacga caactgggtc tggacctaca
    10801 cgccgaccgt cacgcagtag cggcgggcct cgccgggccg cgcggatcta cacgttcctc
    10861 ggtttgggcg tgaacgtcca cgtccagtcg gcgtccctgt agatcagcga cttcctgtcg
    10921 ggcgagacgg tcaccttgct cgacgcgggg agcttcatcc cgaaggtcac cggggacggc
    10981 accacgagct ccgccctgcg cgtcttcgcc gcgccgggga tcggctttcg cgcgccggta
    11041 cagaactgca cgacctcgac gagagggtag gtcgtccgga cgtcgagctc gagcgctccg
    11101 ccgaggccgt tcttcacgct caggacgtcc gcgtgctcgt actcgccctt gagcaccggc
    11161 ggcaggcact tgggctccag gtcgcgcggg ttcttcaggg cgctcttcga gttctggatc
    11221 ctcggcgtgc cgacgaggcc cgatgccgcg aggtcccact tgaagtcgag gatgacctgg
    11281 tcggtgacgt cggcaacccc gctgcccttt ccgggatcgc tcgtgatctg gggcttgccg
    11341 ctgtagacgc cgacggcgtg atagtcgatc ggcccaccgg tccacttccg cgagtcgtcg
    11401 acctgcttct tcgcccccat gacgtcgtcg gggttggccc cgctcgccgc gatcatgtcg
    11461 cggtagacct tctcctgctc gggagtcatc tgcgtctgcg ccacggcggt tccgccggca
    11521 aggaggagcg acgccagaag cacgggaagg gacgcgctgc ggatggatct cggggtcttc
    11581 atggttctct cctcatctgt ggggtcaggt ctaccttcta cactctgcgc aatcctgcgg
    11641 tcgttaccgg gcggaggcgg ctagcggcag tacagcgtcg gcccgatcac gaggcacgaa
    11701 gctccgctgg cgccgatcgt ggtgatccag cccggcccgt ccgcgggccg gagcgcgagc
    11761 aggtagccgt cgagcacgta cgtgccgcgg gcgccggcgg cgatggtcag ctcgctgctc
    11821 gcgctgacgt ggccggactc gtaggtgccg tcgggccgga agacgtaccg gccgcccgcg
    11881 ccgctccagg tgccgaccag gcccttcgcg gccggcggcg cgaggggccg gtgccgcccg
    11941 gctccgatct cgatccacgc gccgtcggcg tcgcttcccg ttcggaacgt gtcggtcttc
    12001 acgaagccgt cgtacgactg cgtgatcctg ccgccggccg cctcgaacgt gccgactccg
    12061 gtggcgcccc tctgtcgctt ctggccctcg acgtcgaaga ggtctccggc gccgggccgg
    12121 gggctgctcg cgcgggtgcc gtcgggcagg aagagcgtca gcatggcgac ggtccggccg
    12181 gagcgagcct gcgggtgcca ccagaggccc tggggacggc cctcgacgag gcgcgcgggc
    12241 ggaatccgga ccccgcgccc gctgggcagc cagcccggcg agcccgggaa gaggtcgggg
    12301 gtttcgccgg tcgggatccg gctcgaggcc gacggcgccg cgggaacggc aggccgggac
    12361 gccggcaccg gcgcggccgg gaccgcggaa gggctgatcg cgaggctgtc gatcaaagcc
    12421 tggacacccg ctccgtactt ctgcagcacg gcgttcgtgc tgaagacgag catcgccacg
    12481 gcgcgccggt tcccgccgac gatcaggacc accatccggg tgtgcgcgcc gaggtccgcc
    12541 gactcgacgt cgaagctctg gatgtgaaac gtcccgaccg cgccgcggtc ggtggtgacg
    12601 agccggcccg agccgacgac cttcgcgtcg gcgttgaccc cgtccgccac ctgcgcgacc
    12661 tgcgccgcgg gcgtcccgct cgccgcctgg acgccgatga cgagagccga catcacctcg
    12721 ccgggcgcga gcctcgaggg cgcgatgacc ggcaggccgt tccgctcggt cgccttccag
    12781 ccctcgggca cggcgaagcg cacgccgttg gcctcgtcgg cggccgcggg cgacgcgccg
    12841 agcgcgagcg cgatcagggc cacgagggtc gacgcccggg cacacgagga gcgcggcggg
    12901 aagagggccc tgatcgtcat gggcgcagca ttccacagga acctagcccg cgccagcccg
    12961 cgatggcgaa gcgcgcgcga gcccgccgcg gaggatgcct ttccttgaaa gccgggcttc
    13021 gatcgccgag actccggggt gccctgcgga tcgacctttc tgaaaggacg atgcggaaca
    13081 tgcgctcctc cgttgtccgc gacgagttga tccgcgccct gcgcctcgac ctgatcgggc
    13141 cggagcccgg gagcgagcac gagcgcgagg agctgccgca cgagccctcg cgctggtacg
    13201 tcggagggtt cctggtgccg ttcgaggcgc cggaggagca gaaggtcgac ccgaccgggc
    13261 aggaagagct gttcgcggcg ggagacgaga acggcgcgga cgacgcggac gtgcccgagc
    13321 gcgcgtcggc gcggaaggtc ttcctgccgt cgtccgtcgg gctgagcgtg ctcgtggcga
    13381 aggggacggg cgcgctcgaa gccgagttga cgtggggcga ctaccggctc ccgaaggcgg
    13441 aggagggcga gccgcggggc agggagtccg cgtggacgcg tgagccgagg cgggccgtcg
    13501 tggcgctcgc tctgcccgag ggtgacgcga aggccgccac cgtggcggtg ccggactcgg
    13561 atggcctgaa cctcgtcgtc tcggggcggg ccgcgggcga agccgaggcg ctggggctgc
    13621 cgaagggaac gcggagcgtt tcggtcttcc tcgtgaacga ccgcaagccg gctcccgagg
    13681 agcggaagga cgaggcggtg gccttccagg tgcggctcgc gctgcgatcg aaagagcctc
    13741 tcgtcgcgcg accgaacgcc acggggatcg actcgaccga ctgggacgac cgcgtggccg
    13801 acctccagta ccgcgacgtc ttcgagtacg cggtggggca cggggtctcg gcgcgggcgg
    13861 aggtcgcgga ggacgggagc tgccgcgagg tggcgaccga gtgggtgccg actgccgccg
    13921 tggagaaggt ggagccgtcg cgtctcgaag gcgtcgagct ggggatggag gcgctcgccg
    13981 cgacgggcga ctccgaggct ctcgcggcct ccctcgcggc gttcccggcg cggtacggcg
    14041 agtggatcgc gcggcagcgg gggacgaagc tcgaggggaa gcgggccgag gcggcggcga
    14101 tcctcctcga cgaggcgaag cacgcctgcg cacggatcga gcggggaatc gccctcctga
    14161 aggagcccga cgtcttcaag gcgttccggg tcgcgaaccg ggccatggcg cgggccgcgc
    14221 gcaggaggag cgcgcgggcg gcgaacgtcg cgccggaggc ggtcgagccg ccgcggtggc
    14281 gcccgttcca gctcgccttc ctcctcatga acctcgaggg gatcgcccgg cccggctcga
    14341 gcgaccggga ggccgtcgac ctcctcttct tcccgacggg cggcggcaag acggaggcct
    14401 acctgggcct cgcggccttc acgatggtgc tccggcggct ccgcaactcg gggctctcct
    14461 ccgccggcgt cgccgtgctg atgcgctaca cgctgcgcct cctgacgctc gaccagctcg
    14521 ggcgcgtggc ggcgctcgtc tgcgcgctgg agctggagcg ggaggcggac gcggcgctcg
    14581 ggacgtggcc gttcgaggtg gggctctggg tggggcgcgc cgcgaccccg aaccggatgg
    14641 gggcgaaggg cgacgacgac gagacgacgg cgcggtcgaa gacgctgaag tacaagaaag
    14701 acagcaagcg ctggccgccg ccgattccgc tcgagagctg cccgtggtgc gggacgccgt
    14761 tcacgaagga ctctttctcg ctccagccga cgtcggaccg accgaccgac ctgcgggtcg
    14821 tctgcgtgag ccggaggtgt gagttccggg gcgaccggcc gctgccgatc ctcaccgtgg
    14881 acgagccgat ctacgcgcgg cgcccggcgt tcctcatcgc caccgtcgac aagttcgcga
    14941 gcctgccgtg ggtgggcgac gtgggacggc tgttcggcaa gggagagaag gagccgctcc
    15001 cgccgcccga gctgatcatc caggacgagc tgcacctcat ctccggcccg ctcgggacga
    15061 tggcggggct ctacgagatc gccgtggacc acctctcgac tcgcgaggag gcggggaagc
    15121 ggatcccgcc gaaggtcgtc gcgtcgacgg cgacgatccg gagggccgag gcgcaggtgc
    15181 gagcgctctt cggccggagc gccgtctacg tcttcccgcc gccgggtccg gaccggcgcg
    15241 actccttctt cgcgcgtacg gtcgcgcagt cggagagaga accccggatg tacgtcggcc
    15301 tctcggcgca ggggcggagc ctgaaggtgc ttctcctgcg ggcgtatctc gcgctcctcg
    15361 gcgcctcgca gcggctcttc gaggaagccg ggggcgcctc gctcggcgag aagaaccccg
    15421 ccgacccgta cctgacgctc ctcggctact tctcgagcct tcgcgagctg ggcggcacgc
    15481 ggcggatcgt ggaggacgag gtggcggcgc gggccgcgcg ctacggtgag cggcggcgcg
    15541 cgggagaagc ggccggggcg ttccgcgacc ggacgatcca gcaggaggtc gtggagctga
    15601 cgtcgcgcga gtcgacggcg aaggtggcgc gcgccaagga gcggctggcg ctgccgttca
    15661 cgagcaggga tcacgtggac gtggccctcg ccacgaacat gatctccgtg ggcctcgaca
    15721 tcacccggct cgggctcatg gtcgtcctcg ggcaaccgaa gacgacgtcg gagtacatcc
    15781 aggcgacgag ccgggtgggg cgcgacgacg cgcggcctgg cctcgtcgtg acgctccaga
    15841 acgtccaccg cccgcgcgac cgctcgcact tcgagcggtt cggggcgtac cacgcgtcgt
    15901 tctaccgggc cgtcgaggcg acgagcgtga cgcccttctc ggcgcgggcg ctcgaccgcg
    15961 gggccgcggc ggtggccgtg gccctggcgc ggcacgggct ggcggcgttg acgcctgcgg
    16021 cgggagcggg acaggtcgag gcggtgcgga aggacctcgc cttcgtcggc gaggagatgg
    16081 ccgagcgggc atcgcggcac gacacggaga agggggccga ggagctggag gctctccgca
    16141 agagagtgaa gagcctcgtg gacgacctcc tcgacagctg ggccgtcctc gcgcacgaga
    16201 agaggcagaa cgacgtgaag ctcgcctacc aggcgaagga gcgcggcggg ccgccgaacg
    16261 ccttcgccct gctccgcgac ccgctcgacc cggagctgga gtcgctcccc tcttcggcgc
    16321 ggaagttccg ggcgcagcgg tcgatgcgcg acgtggagcc gagcgtccct ctcgttctcc
    16381 ggaggctcga cgacgtcgag gtgcccgacg ccgaggaggc cggctcgtga agaagaggca
    16441 ccacggcgag atccgccgca gccagctcgt gaggacgttc gggccgggtg cgatggtcga
    16501 cctcccgaat cactccgtcg tcgtgagcgg tctcgactcc tggttcccgg cctcggagat
    16561 gccgacgatc gtcgaggagc gcctctcgct gaaggtggcg agcgtgctcg ggcacgggaa
    16621 gcccgtgccg ctgaagggcc ccccggtcgt ctccgacgac gagcggacgc cggccacggg
    16681 cgtgccggcg ttccagttcc ccgagtggtt cgtggtgcag gtgaacgcga agcggcggcc
    16741 gctcgttcac ctcgagagcc tcgacaaggg gaagtacgag gtcgaccgga agaaggtccc
    16801 ggccgtcccg gtccgcttcg tccaggcatg cgtcctcggg catctctccg acatcgactg
    16861 gcggggcttc attcacatgt accggggcga ctgctcccgg ccgatctgga tggaagagcg
    16921 cggctcgtcg ggagacctcg cggacgtcgt cgtcggctgc gactgcgggg ccgaacgctc
    16981 gatggcgcag gcgacgcggc ggggcgccga gagcctcggc gagtgcgccg gccggaggcc
    17041 ctggctcgga cggcacggcg gggagcggtg cgggggagag aacgggaaga ggcagctcgc
    17101 gcggctcctc gtccgctccg cgagcgacgc ctacttcccc caggtgctga gcgtcatctc
    17161 cctgcccgac cgtggctcgg tcgtccgcga cgcggtggcg aaggtctggg agaacttcct
    17221 ctcgctcgtc gaggaggcgg gcgagctcga gaaggagaag aagcgcccgg cggtgaaggc
    17281 cgcgctcgag ggcctcacgg acgaggaggt ctggaccgag atcgagcggc ggaggacggg
    17341 tgcgcccgcg cagacggaga agatcaagga ggtggagctg gcgacgctcc tctccgtgaa
    17401 ggagaagctg ggcgacgacg tccacagcgg gcacgacttc ctcgcgcggc ggctcccgct
    17461 cgaggcgacg accgggccga tgatggcaat cgagcgggtc gtcctcgtgg accggctccg
    17521 cgaggtgacg gcccaggtgg ggttcacgcg gttcgaggcg cagacggccg acatcgacgg
    17581 cgagctttcg ctcgacgtga ggacggcgcc cctggccgcg gaggtgaaat gggtgccggc
    17641 cgtcgagaac cggggcgagg ggatcttcct cgcgctcgac ccggcccgca tcggcgcgtg
    17701 gctcgagagg cccggggtcg tggagcgggg gagccgcctc tcgcgcggct tcgagcgctg
    17761 gaaggcggcc cacaggaagc ccgcgatgcc gttcccgggg cttccgtacg ttctcctcca
    17821 ctcgctttcg cacctgctcc tgacggccgt ctcgctcgac tgcggctacg cgacgagctc
    17881 gatccgggag cggatctacg tgggcgacga ggccttcgga atcctcctct acacgtcgag
    17941 ccccgacgcg gaagggacgc tcggcggcct cgtggaggtg gggcggaacc tcggccggca
    18001 cctgggccgc gcgctggatc tggcccgcct ctgcgccagc gacccggtct gcgcgatgca
    18061 ccggcccgac agcggacacg aggagcggtt cctcacgggc gcggcctgcc acggttgcct
    18121 gctcgtcccg gagccgagct gcgagaacag gaacgacttc ctcgatcggg ccctcgtggt
    18181 gccgacggtc ctcggcggcg acgccgcctt cttcgcggga gacctggcgt gaaggggccg
    18241 tgggaggagc tctcgcgggc ggggctcgaa ggcctcgccg gggcgctcgc cgcaggtcga
    18301 atctccgggc cgtactcggc cggcgccctg ggccgccacc ttccgggcgt cgacctcgcg
    18361 aacgccctcg cggagctgaa ccggctccac gacgcgggac ttccacccgc gcacctcgcg
    18421 gaggtcctgc gggccgtcgc ggccgagcgg aagctccgcg aggtcacatc ggaccgtgtc
    18481 gagctcgtct ggacgggacc ggagatgccc gggagccaga gccgcgagac ggcgatcgtg
    18541 gcgcgggagc tgttcgcgag ggcgaagcgg aggctcctcg tctcgagcta cgcggtcttt
    18601 ctcgggaagg acgtcttcga gcctctcgcg aggaggatgg aggaggtgcc cgacctccgc
    18661 gtcctcctgt tcctcaacgt cccggccccg ggcggagcgg acttcgaccc cgacgccgtg
    18721 gcgcgcgagt tcttccgcga gttcctgaag aagaactggc ccgccggccg tcagcccgag
    18781 ctctactacg acccgcgatc gctcgccgca aaggccgacg agcgcgccgt catccacgcc
    18841 aagtgcgtcg tcgtggacgg ccacaccgcg ctcgtcacct ccgccaactt caccgaggcg
    18901 gctcacgccc ggaacatcga ggccggcgcc gtcgtccacg attcgggctt cgccggctcg
    18961 ctggaggcgc agttcacgtc gctcgtcgcg gcggggaagc tgcggcgcgt cttcccgtag
    19021 tccggttcgg ctgccgttcg gccgccgtcg catgccgcgc gtaacatccg ccgccgaaat
    19081 gagcagcgtg acgaggcgag cgggagcgag ctgcatcgcg ggagcggtcc tcctcctgac
    19141 gctcacgctg gccgacgggg cggcagcggc gacgctgaag gacccgcgcg gggcgatggg
    19201 ctcgaagttc gagatcacgg cggtccacgc cgacgaggcg aaggggcgcg cggcgatcga
    19261 cgcggcgtac gccgagatcg aacggatcga ggggctcctt tcggagtgga gggagtcctc
    19321 gaaggtgagc gagatcaacc ggcaggcggg ggtcgctccg gtcgaggttc cgaaggagct
    19381 gcttcacctc gtccgccgct cgctgaaggt ctcggcgctc acggacgggg cgttcgacgt
    19441 cacgttccac accgtcggac gcctctggaa cttcaagtcg cgaacggcgc ccgtcccctc
    19501 ggaggaggcg atccgcgcgg ccctcgtcga cgccggctat cggcacgtgg tcctcgacga
    19561 ggggaaatcg accgtcttcc tcgaccggaa ggggacgcgg atcgggttcg gcggcatcgg
    19621 gaagggctac gccgcgaacc gggccgtctt cgtcctgaaa gagcacggcg tcacgggcgg
    19681 tgtcgtgaac gcggcgggcg acctcgtcgt cttcggcacg caggagagcg gcaagccctg
    19741 gcgcatcggc atcgcgaacc cgctcgagaa gggccgcgtc ttcgcctggc tggagacgac
    19801 cgagcaggcg gtcgtgacgt cgggcgatta cgagaactac gtggagctcg gcgggcggcg
    19861 ctactcgcac atcctcgacc cgcgaaccgg ctggcccgtg accgagctgc gcagcgtgac
    19921 ggtcgtctgt cccgacgggg aggtcgccga cgcgctcgcg acgggcgtct cggtcctcgg
    19981 cgtcgagaag gggctcgcgc tggtgaaccg gctgaacggc gtcgaggcga tgctcgtcga
    20041 cgcgaacgga aagctccatt tctcgagagg cctcgaagcg atgttgacga aggaaggcgg
    20101 cgaccgttga aaagacgact cccggtgatc ctcgccctgg tcgggctctc ggccctcttc
    20161 ccgggctgca cgacggtgca gccgtgggag cgcggggcgc tcgcgaccga gccgatgaag
    20221 ctcgacgact gccggacgca ccgcttcgag cgcgcggccg agacctaccg ggagggcgcc
    20281 gtcggcggcg ccggcggaaa gtcgggcggg ggatgcggat gcagctgagc cggctcgcga
    20341 ggctgctggg cgccgccgcc ctcgggacag tgctggcggc gacggctccg gcggcgctcg
    20401 ccggggagcc gcccgaaggc gtcctcgacg ccgacttcct cttcggctac tacgaccagg
    20461 acggcgaccg ctctcccgtc accggaggcg tcggcacgga gaagctccat gtctactcgc
    20521 ccgtcctcca gctggcctgg cgcctgaacg agtccttctc gctctcggcc gacgtcggcg
    20581 tcgaccaggt ctcctcggcc tccatgggga acatccagtc ggagctgagc agcgcgagca
    20641 tcccggccag cgaccagcgc ttcttcggga cgctccgggc gaagaagaag tggaagagcg
    20701 gcacgtgggg gctgaccgtc ggggccgcaa aggagtacga ctatcgctcc ttctcgtacg
    20761 ggatcgactg gggcctcgag ctgaacaagg cgaacacggc cctctccgcc tccgtgcggc
    20821 gcttcgacga cgccatcgac ctgatcggca tcgacggcta cgggtaccag ggcccgggtc
    20881 tcccgatcac caccggcagc ggagaccgga cgacgaccga cgtcacgctc ggcgtttcgc
    20941 agacgctcgg ccggcggacc gtcggctcgc tcgagctctt cctgagcagc caggacggcg
    21001 tcctctcgac gccgtatcac gaggtcgtcc tcgggccgtt cgagcccggc gccgttcgcg
    21061 tggccgagcg cctccccgac agccggcttc ggaaggccgt cgccctccgc ctcagccact
    21121 ccttctccga ccggctcgtc ctccgctcgg gctaccgctt ctacacggac gactggggca
    21181 tctctgccca cgccgtcgag ctcgagcccc acttcaggct ccggtcggag cgggagatgt
    21241 ggctcttccc gatcctccgc ttcagcacgc aggcgggcgc cgactggttc ggcacgcccg
    21301 gcacgttcac cggcaccgag acctactaca cctcggacgg ggacctcgcc gaaacgacga
    21361 cgcagaagta cggcgtgggc ttcacgctca acaccaagcc cgggcagacc tggctcggca
    21421 tcttccggag gttcgaggcg cgcgccgcgg tctacgaccg cgacgacggc ctgacggcct
    21481 tctccacgtc gttctcgttc gggtggacgt actgatgcga cgcgcggccc tcttgctcgc
    21541 gctcctcctc gcgcctccgc tcgcggcggc cgagaccgac cagcacggcg tcgcgcaggg
    21601 gtgggccggc gcgtcctccg gctacaccgt cctcgacttc gccgcctcct ggtgcacgcc
    21661 gtgctggagc gtcctccctc gtctcgagaa gctcgcggcc gagcacccgg aggtgcgcgt
    21721 cgtcgtcgtc agcgtggacg acaaagtctc ggggcgagac ctcctcgtct cgaagctgaa
    21781 gctgacgatc cccgtcctct gggacgggaa gcaccggatc gccgagcact acaggcccga
    21841 gggcatgccc cacaccgtcg tcctcgatcc cgccggcaac gtcgtccacc gccacaccgg
    21901 ctcggggccg aaagagtggg aggcgatggt ggcgttcctg gcgaaggcca cgaagcgctg
    21961 aacggagcgc cgtccttctc ctacaccttc tcggcgacga ggagggtgat gtcgtcctcg
    22021 aggaagagct tctcggcgtg gccgaaaacg ccggcccgga gcgcctcgat gatcccgtgc
    22081 gccccctcgt gcgccgcgtc ggcgatgacg tccctcaccg cgtcgacgcc ccagaggagc
    22141 ccgtcggggc tcgaggcttc gtagaggccg tccgtcgcga ggatcacgag gtcaccgggc
    22201 gagagaggct cgatgtcgaa ggcgcgatac gtcgtcgtcg tcgaccagcc gaggacgagg
    22261 tcggtctcct cgaggcggtc gaacgagcgg gagcgggccc ggtagacgag gggcggaagg
    22321 tggccggccg agacgtagca cgcttcaccg gcgggctgga tccggcaggc gacgagggtg
    22381 gcgaaggcgt cgtcggggat gtccggcgtc atgagccggt tgagcttctc gaggagcctc
    22441 tgcgggtcgg cctcgacgag gtggagggcg cggaggtagg cgcgcgccgt cgacatcagg
    22501 acggtggagg cgatcccatg gccggagacg tcgcagacga ggaggtcgag ggccccgaga
    22561 ggcgcctcca tgacgtcgta gtagtcgccg cccgtccgct cggccgtctc ctgccacgcg
    22621 gcgagacgga ggcccggccg ctcgggcagg acgcgcgggc gcaggccttc ctgaatcctc
    22681 cgggcgagct cgagctcgct ctccatccgc tcccgacgga gctcgccctc caggaggcgc
    22741 tgcgtctcga cggcgagggc ggcgtggctc ccgagggcgt gcagcgcctc ggcgtcgtcg
    22801 gcgtcgaacg cggcgtcccc cttcttgttc atcgcctcga tgacgccgac gacggagccg
    22861 gacggcgtca cgagcgggac gcagaggacg gagcgcgtcc ggaagcccgt cttcaggtcg
    22921 atgtcgcgcg agaagcgcgg gtcctcgtag gcgtcgggga tcgagatcgt ctcgccggtc
    22981 cgcgcgacga tcccggcgat gccgaagccg ggagggaggc ggatgtcgga ggaatcctgg
    23041 gcgacgcgcg tccagaggct tccgtccggc tcgaggagga agaggctcgt ccggtcggcg
    23101 tcgacgagcc ggctcgcgga ggcgacgatc agctcgagga ggggctggag ctggcggacg
    23161 cccgtcatcg cctgagcgac ctcgaggagc gtgcgaaggt cctggagcct cttctcgtcc
    23221 gtcagtgcca tggcgtcgtg cgtcatgtta ctttcgccgg tcgggggttt cgacaccatg
    23281 atcgagacgg tgaagcagat tctgatccgg gcccgggagg ccggccggag cgccctcctc
    23341 gagcacgagg tctacgaggt ccttcgtgcc gcggggctcg acgtcccccg gttcgcgttc
    23401 tgggaggggg agccggccgg cgaggcgccg gccgccgtcg ccgcgctcct gtccggggga
    23461 gcggcggagg tggtcctgaa gatcgtctcg cccgagatcc tccacaagtc ggacgtcggc
    23521 ggggtcgcga tcgtcgccgc ggagccggcg gccgtcgccg cggcggcgcg gaaggtctgg
    23581 gaggacgtgg ggcggcggat gccggacgcg tcgcgctcgg gcgtcctcgt cgtggagaag
    23641 gtgaagacga agggcggctc gcccgcctcc gagacgctcc tctccctcaa gcaggacccg
    23701 gccttcggcc cggtcctcgt cctcggtctc ggcggcgtcc tgacggaatg gttcggggcc
    23761 gccacgggcg ggacgtcgac ggtcgtcctg cggcccggcc gggtccgcga agggctcgag
    23821 cgggcggtcg cgacgcggcc ggccgccgcg ctcctcttca agccgagccg tctccacgat
    23881 cgggccccgc tcgacctcgc cgccacggcg gagcggctcg agaggctcgg cgctgtcgcg
    23941 acggcgttcg gggagggagc tcaggccggc gaagcggacc ccctcacgct cgaagagctg
    24001 gaggtcaacc cgctcctcct cgccgccgac gggcgctgga tcgcggtcga cgggaagggg
    24061 agcatgggcc gctcgacggc gacgctcccg aagcggccgc ttcggaaggt gaagaacctc
    24121 ctcgagccga agtcggccgc cgtcctcggc gcgtcggcca cggcgatgaa ccccggccgg
    24181 atcatcctcc ggaacctgaa gctctccgag ggggtccgct atggacacat ctgggcggtc
    24241 cacccgaagg aggaggcgat cgacggcatc ccgtgcgtga agacggtcgc cgagctgccg
    24301 cagaaggtcg acgtcgcggt cgtctcgata ccggcggagg gtgcgaggga cgcgatccgg
    24361 gcgctttgtg acacggggaa ggccgagtcg atcatcctga ttccgggcgg cttcgcggag
    24421 acggggaaga aggcgctcga ggaggagatc gtctcgatcc tccgcgcctc gcgctcccgg
    24481 gccgacgagg ggcccgtcct cgtcggcggg aactgcctcg ggatcgtctc gaagcaccag
    24541 tacaacacgt tcttcctgcc gcagtacaag ctgccgttcc acgacgcgcc gggcgaccgg
    24601 ctcctggccg tcagccagag cggcgcctac ctcgtctcgc tgacgtcgaa cctggacggg
    24661 atcatcttcc cgaaggcgtc gatctcgtac ggcaaccaga tggacctgac gatctccgac
    24721 ttcctcgagg cggctctcga ggacgacggg gtggacgtcg tcgcctgcta cgtcgagggg
    24781 ttcaagcctc tcgacgggac gcgcttcgtc gacctggcgc ggcgcctgcg cgagaagggg
    24841 aagcgggtcc tcgcgttcaa ggcggggaag accccgctcg ccgcgaaggc gacgcagagc
    24901 cacaccgcct ccctcgcggg ggactacgcg gtcgcggagg ccctcatgaa gggggccggc
    24961 gtcgtcgtcg cggagaccct cgacatgctc gaggactaca cgaaggcgtt cacgatgctc
    25021 tacgaccggc tcccgcgcgg gaaccgggtc gccgtcctct cgaacgccgg gttcgagtgc
    25081 tcctcggtcc tcgacaagct ctacggcctc gtcccggcgc cgctcaccga cgagacgaag
    25141 gcgaagctcg aagcgtgcct gccgggcgtc ggccacgcgg ggaaccccgc ggacgccacg
    25201 ccgatggcga cgacggcgca gttcgtggcc gcggcgcagg cgatggtcga cgacccgaac
    25261 gtcgacctcc tcctcgtctc gcccgtcccg gtcacgcccg cgctggacaa cctcgcgccc
    25321 gacctctccg gcacgcaccg cgagaacatc cactcgcccg gctcgctccc gaacggtctc
    25381 ctggagctct tcgggagcac gacgaagccg gtcgcggtcg cggtcgactc ggggcgcctc
    25441 tacgacgact tcgtcttcgt cctccagcgg ggcggcatcc ccgtctggcg gaagatcgac
    25501 cgcgcctccc gcgcgctctc ggcgctcgcc acgctctgac cggcgggcac gaagagggaa
    25561 aggccccgga cggcggcgag ccggccgggg ccttcgtcct tcccggggga agggccgggg
    25621 ctcagagccc gtagtagagc tcgatctcgt acgggtgcgg gcggttgcgc acctcgcggt
    25681 cttcggcccg cttcttcttc gcccaccgct cgaggaggtc gtcgttgaag acgcctccct
    25741 cgaggaggaa cgcgcggtcg gcctcgagcg cctccatggc cgcctcgagc gaggtgggga
    25801 gcgccttgat cgtggcccgc ttctcggcgt cccacgagaa gatgtcgtcg tccagggggc
    25861 cgaagccgag ggcggtcggg tcgagcccct tgcggatgcc gtcgacgccc gcgaggagct
    25921 gggccgcgat cgcgaggtag gggttgcagg tggcgtcggg cggacggaac tcgaagcgcg
    25981 cgttgtcggg acggttcgcg tacttcggaa tccggatcgc cgcgctccgg ttgccgagcg
    26041 agaagatggc gctgatcggc gcctcgtagc ccgggacgag ccggcggtag gagttcgtcg
    26101 aagggttcgt gaaggccatg acggcgccgc cgtgctggag gaggcccgcg atgtacgagc
    26161 gctccgcggc gctcgaggag ccgtagccgt tcgcgtcgta gaagaggttc ttcccgccct
    26221 tgaggaggcg ctggtggaag tgcatgccgg agccggcctc gccgtagagc ggcttcggca
    26281 tgaacgtggc cgtcaggccg agctgcgtcg ccgccatccg ggtgacgtac ttgacgatca
    26341 tcaccgcgtc ggccgccttc atcatcggga agatcgggat ctcgatctcg cactggccgg
    26401 ggccgcccac ctcgtggtgg tggtacttca cctcgatgcc catctgcgag acgagcttgc
    26461 agatgcgcgt ccggaagttg aacaggcggt cctgcggcgg gatcgcgtgg tacccgccgt
    26521 gcctcgggat gttgtacccg gtgccgagct cgtgggagtt ccagtccccc tcggaggact
    26581 gcacgcggta ggaggcgacg ttcatcccgt tctcgtacga gacgccgtcg aagacgtaga
    26641 actcgaactc cgggccccag acgctctcgt cggcgacgcc gctcgcgcgg aggaactcct
    26701 cggcccggcg ggcgatggag cgcgggtcgt aggggaagag ggcgcgcgtg tcggcctcga
    26761 gcgtcgtgca gatgaacgac agcgtcggga cctcccagaa cgggtcgagg aagcccgtcg
    26821 cgaggtcggg gacgagggcc atgtcgccgg agctgaccga cttgaagccg acgctcgagc
    26881 cgtcgaaccc gacgcccttc tccatcagga cgggggtgaa gcccgaggcg gggatcgtga
    26941 cgtggtgcca gcggccccac aggtcgcaga acttgaggtc caccatctcg acggaccgct
    27001 tctcgacgaa ggacttcgcc tcgctgaagt cgctgaacat gacgggtctc tctcccttcg
    27061 gcacgccgca cgagaagacg cggcgcggcc gactatggta acgccggagg aggggggctg
    27121 tcccgccggc gaaggcgcgc cggctgctac gatgagaaat tcgggctcgc ccggacggcg
    27181 ggccctggag tagacgatgc ccatggaagt ccgtttcccg ggcggggtcg ccgtcgaggc
    27241 gatccacgcc ggaatgacga tccgaaccga tcagcccgtc gcggccggcg gggccggctc
    27301 ggccccctcc ccgttcgacc tcttcctcgt ctccctctcg acctgcgccg gcttctacgc
    27361 cctccgcttc tgccaggagc gcggcctgcc gacggagggg ctcggcgtca cgatggactg
    27421 ggagcgctct cccgagacga agctgatctc gaagatccgg atctccgtga agctcccgga
    27481 ggggttcccg gagaagtacc gcgccgcgat cctccgcgcg acggaccagt gcgccgtgaa
    27541 gaagcacctc gtctcgccgc cggcgatcga ggtcgaggcc gtctgagggc cggggcctag
    27601 gagagcgtct cgaagacccc gagcccccgg ttcttccgga cgtcgttcca ggggaagtag
    27661 gtgccgttca tggcgacgta gacgccgtgc gggaggacct ggacgaacga gagggcgctc
    27721 ccgaggttga agaggccgtc ggagctcccg aaggcgtacg ggatcatcgc gccggtcagg
    27781 acgatcgtct tcggcaggcc gaccgcgccg aggacgcgcg cggtctcttc catcgtgtcg
    27841 gtcccgtgag tgatcacgat ctgctcttcc tcggacttgc ggcagatctc ctcgatgatc
    27901 ttccggtccg actcggtcat ctcgaggctg tcgaccatca tgaccgtccg gacgcgcgtc
    27961 tggagccggc aacggcccag cttcagcatt tcgtggatgt gggtgtcccg gaagtagagc
    28021 ttcccggtca gctcgtcgta gtccttgtcg aacgtgccgc cggtgatgag gagcttgatc
    28081 gccatcgatt cccccggacc tcgatgatag tgcggacgcg agacgccctc cggattctcc
    28141 ttgtacggac ccgcgatctc cgctacgttc cgcccaccgc ccggatgaat gcgcgatcgg
    28201 agagggaccg gcctcttcgc ggaggagaag tatgaaggtc aggctgggta cggcgatcct
    28261 cgtcggagct ctcgggctgg cgcacgggac ggtgtccgcc caggggaaac ccgcgtcttc
    28321 cgtcaagaag ttccgcttca aggcggggaa gatcgaaacg ggtcgtctct acgcctacga
    28381 tcggttcgac ccccggggag gccgggcggg gagcgtcctc gtcttcgtcc ccgaccgaaa
    28441 gagggtcgag gtcttccgcg tgaaggcggg ggcgaccgac gggcagctct tctccggcga
    28501 gatgaactgg gagacgttca cgcttcggac attcgagctc tcgaagctct cccgcgacgg
    28561 gacggcgacc cgccaggcca ccgggacgtt cggcgcggat tccctggccg tgtcggtcga
    28621 ggaccccgct ctctaccgtg gagcggaggg ggcgaccgct ttcacggtcc cccttccggc
    28681 gatcccggcc cacgtctaca gcctcgacct gatcacgctc gggctcgccc tgcggcacgt
    28741 ggccgacccg accggggccg tggagttcgg cgtcctgacg gagaacccga aggtcgggcc
    28801 ggagagcccg ggcctcttcg tcgcctcggg gacggccacg gtggagttcg tcgaggacgt
    28861 gacgcgcgac ggcgtcccct gctccaagta ccgggtctcg ggcccggcgc tcggcggcca
    28921 ggaaggcttc gtctggctcc acaaggacga gggttacctc caggacgccg agattcccgt
    28981 ctccccgacg cccgacctcc cggacgtgag gatcacgctg cggtcggtcg acaagctgtc
    29041 ggagaaggac tggccggcgc gccgcctcct cgagctctcc ggcgggccga agtagagccg
    29101 cggccccgat ccgtcaggag accatcggca ggaggagagg cgcctcaacg aggaacggca
    29161 ggctcgcgat ctcgtcgaga gcccgggcga cgcggtcctc cggcgtcggc tcgagcgtca
    29221 cgacgaacgg gagggcgcgc ttctccgtcc aggggacctg gaggacggcg tcgacgttca
    29281 cctcggcgcg ggcgaacgcc gcggtgaggg cggcgagaac tcccgggcgg tcgtccacga
    29341 cgaatcggac gtagaacggc gcgacggaat cggcgggccc cccggcgacg cagggacgcg
    29401 gcgcgtccgt cccgaacggc gggacgagga acggggcgcc gcgccgggcg atctcgacga
    29461 cgtcggcgag gacggcggcc gcggttggcg cgcccccggc tccgggcccc gaaaggacgt
    29521 actcgccggc gtgcgccgtc gtgacgcgga cggcgttgag cggcccgtcg acccgggcga
    29581 ggagcgagga ggccggaacg agagcggtcc ggacggcgag gaggaggcgg ccgtccggga
    29641 ggcgccgcgc gaggccgagc tggcgcggta cctgcccgag gagccgcgcg tagacgaagt
    29701 cgaccggccg gagccgggtc acgccctcgg tccggaccac ctcgagcggg acgtcggcgc
    29761 cgaacgccat ccgggccagg agcgcgacct tgcaggcggc gtccgccccc tcgacgtccg
    29821 cggtcgggtc ggcctccgcg tagccgaggg tccgcgcctc ggcgaggact tcggcgtaag
    29881 gccggccggt ccgcgccatc tctgtgagga ggtagttgca ggtgccgttg aggatcccgc
    29941 cgatctcctc gatgcgatcg gagacgaagc tctcgcggag agcccggagg atcgggatgc
    30001 cgcccgcgac cgcggcttcg aaggcgaggg ccgctcccgt ccggctcgcg aggcgggcga
    30061 gctcggcgcc gcgggtggcc aggaggagct tgttggcggt gacgacggat cggcccgccc
    30121 ggagcgccga ctcgacgagg gggcccgcgg ggtcgaggcc gccgaggagc tcgacgacga
    30181 ggtccacgtc cggcgcgagg gcgacggcgg agaggtcctc gccgacgcgg actcccggcg
    30241 ggatcgtcga gaggagctcg gaccggctgg ggagggcgcg gctcgcgacg gcgacgaggc
    30301 ggaggtcgac cccgtgcgcc tccgcgagac gcggtgcctc cgaagcgaac aggcggacga
    30361 gctcccgccc cacggcgccg caaccgagga gcccgatccg gagggtcggg gtcacggcgc
    30421 cttcggcagc caggagtcgt ggtagtcggg gagctcgagg gcccgcgcct gttccgtgac
    30481 gcgaaggggg aggaaggtgt cgatcatgac ggcgagctcg tcggtcgacc gcgtgccgat
    30541 cgacgcctcg taggcgccgg ggtgcggacc gtgcggaatc ccggccgggt ggagggagac
    30601 cgcccccggc ccgacgccgc ggcggctcgt gaagttgccg cgcacgtaat agatcagctc
    30661 gtcgcagtcg accgaggagt ggggataggg gcacgggatc gcctcggggt ggaagtcggt
    30721 cacccgcggg acgaacgagc agacgagcgc cccgcgcgcc gcgaacgtcg cgtggatcgt
    30781 cggcgggagg tggacgaggc cggtcttcgg ctggaacgcg aggatcggga acgcgaacgg
    30841 ccagacggtc ccgtcccagc cgacgacgtc gaacggcgag acgaggggct cgcggcgaac
    30901 gaaggcgtcc cccttcttga cgaggacggc cgtcggcccg ggccgggggc cgcggaagac
    30961 gggccgccgg aagtcgcggt gcgtgaacgg cgcgtccatc cggagctggc cgacggtgtt
    31021 gcggtactgg gtcggaacgt ggaggccgcc ccggagctcg aaggcgatgc cggtgatccg
    31081 ctcgtcgacg cgccaccggt ggacgacgct ccgcgggacg aggacgtagt cccccgcgcc
    31141 tgcggcgagg tcgccgaacg gggtctcgag cgtcgccttc cccgagaaga cgtagaggag
    31201 gtcgtccccg tcgccgttcg cgaaacccgt ctcgggggcc ggggcggccg ggaggtcgaa
    31261 gagggagacg acgacgtcgg cgttgccgca gacgggcgtc cagccctgcg gcgtcctgtc
    31321 gccgaggagg agccggcggc ggagcggctg gtcgggcgcc cacgcgggga cgcggcggtc
    31381 gagcggtccc ggctccgcgg gtccgatcga ccggtcggtc atcggcgggc cctcgtggta
    31441 gaggatcgag tactcgccgt cgaagccgag acgggtgaag cactcctcga agcggagcgc
    31501 gcccgaagcc gagcggaacg cgacgtgggg cttcgccggc acgtcgccga gctgcatccg
    31561 gtcgatcatg tcagccgacc acctcgaggg aggtcgcgtc ggtgctctgc gccgcctcga
    31621 tcgactcgaa gagggcccgg aagttcccgt agccgaagcc cttgtccccc tcgcgctgga
    31681 tgacctcgta gaagaagggg cccgccttcg cgtcgccgta gagcgtgccg gcgtcgtgca
    31741 tgaagatctg gagcatgtag cggtcgtcct taccgtcgac gaggacgtgg ttcgcgcgaa
    31801 gggcgtccat cggctccttc acgttcgaga ccttcacgcg ctcgagccgc tccgggagcc
    31861 ggtcgtagta ggccggcggc gccgagagga actggacgcc tcgcgaacgg aggctctcga
    31921 ccgccgggag gatctccttc acgaggaggg cgacgtgctg gacgccggcg ccatggtggt
    31981 cctcgcagaa ccggttgatc tggctgtcgc ggaagaacgg gcgcatcggc tcgttcgtcg
    32041 cgaacttgat cccgctcgac gggtcggcca tgacgatgga gcgcaggccc gagcccgtca
    32101 tcttcctccc gccgtcgacc tcggacgtgt ggaactcgac gtcccagtag cgagtgaagc
    32161 cgaggacgtc gcggtaccag ttgacgacgg ggagcatcgt gtaggcgttc gaggtgacgt
    32221 ggtcgatcgt cgcgaagccg tgagggttgt cggacgggag gccgtcgccg acgttctcga
    32281 agtcgggggc gaaggagggg taggtccctc gcctctcaat gaagcggaac gtgacgtcgt
    32341 cgagcggcgt ggcgatcgag aacgtccgga agtagccgcc gccctcctcg tggacctgga
    32401 cgtcggtgag gaacgtggcg ccgcggggct cgaggaagcg ccaggcggcg tcgacgtccc
    32461 gtacccggaa cgcgagcgag gagacgccgt cggggtggac ccggagccag cgggcggctc
    32521 tcgaatcggg acggaccggc gtcgaaacga ggacgcggat ctggcctgcc ccgaagacgc
    32581 tcgcctcctc gccgccggcc tcgaccttcg cgcgcgacgc gcgggcgacc tcggtgaagc
    32641 cgagggcctc ggtgtagaag cggcggctcc gctcgaggtc gccgacgatg aagtggaacg
    32701 agtcgtatcc gacgatgccg agttcctggc tcatgagacg gattctagga gggttcggcg
    32761 cgggcgggag aacggacggc ccggccgtcc gacgccgcgg gccgaggccc cgtaaaatcg
    32821 ggacgtggag gcgaccccga cccagacgcg cccgagcgag ttcttcctgc gcgacctgcc
    32881 tctctacctc gattacctcg cggtggagaa ggggctcgcg cgcaactcgc tcgaggccta
    32941 ccggcgggac ctggccgcct tcggccgcca cctcgacgcg gagggggtcc cggccgcgga
    33001 ggtgacgcgc gacgtcctcg tccgctggtt cggcctccgg cggtcggaag gggcttcgcc
    33061 ccggtcgatc gcgcgggcca cgtcggcgct tcgcggcctc ttccgcttcc tcttcgccga
    33121 gaagcggatc gccgcggacc ccacctccgg cctcgagaac ccgcggcgct ggatgaccct
    33181 cccgaagctg ctcggtcgcg acgaggtgga gcgcctcctc gaggcgcccg acgtcgagac
    33241 cccgaaaggg cttcggaaca aggcgatgtt cgagctcctc tacgcgtgcg gggtccgggt
    33301 ctccgagctc gcgacgctcc ggatcgcctc gctccacctc gccgacgggt tcgtcgtcgt
    33361 gaaggggaag ggctcgaagg agcgggtcgt cccggtcgcc gactcctcgg cgcgctgggt
    33421 cgcgcgctgg gtgaacgggc cccgggcccg ggcgcgcggc gccgcctcgc cctggctctt
    33481 tccggggtcg ggcgggaagc cggtgacccg gcaaacgatc ttcctggcga tcaaggcggc
    33541 ggccctgaaa gccggcctcg acccggcggc agtctccccg cacgtcctca ggcactcgtt
    33601 cgcgacgcac ctcgtcgacg gcgaggccga cctccgggcc gtccagatga tgctcgggca
    33661 cgcgagcatc gccacgaccg agatctacac ccacgtctcg cgggcccggg ctcgacgtgt
    33721 ctacgacagg agccatcctc gtgcgtaggc tcctcgcggc ggcgtccctg gccctcctcc
    33781 tcccggccat gccggccgtc tccgctctcg tcgtcctgac cgagggacgc cacctgaagg
    33841 cggcttcgtt ccatctcgag ggagaggagc ggatccggat cgtcctcgtc ggcggcggag
    33901 aggtcgtcct cccgatcgag cgcgtggagc ggatcgtcga cgacgagctc gagccgcccg
    33961 ccgcggtcga ggagcccgtc gcgcccgcga cgaaagcgtg gccctcggtg cgccggccgt
    34021 ccggccggcc ccactccgcc cccgcccgct gggcccggct tgtcgaggag gcggcggaag
    34081 cccaccaggt cgacccggcc ctcgtggcgg cggtgatcca cgtcgagtcg agctggaagc
    34141 cccgcgccgt ctcgccgaag ggggcccgcg gcctgatgca gctgatgccc gccacggcgc
    34201 gccggctcgg cgtgtcgcgc tcgttcgacc cggcccagaa cgtcggaggc ggcgcgcgct
    34261 acctttcgct ccttgcgaag cgcttcgggg agaacgagcc ggagaagatc ctggcggcct
    34321 acaacgccgg ggagggagcc gtcgaggcct acggggggat tccaccgtac cgcgagacac
    34381 gcgcctacgt gaggaaggta atcgccctgt ggacgggcga gccggccggc tcctgacgcc
    34441 cggcctccta tactcgcccg cgtgtttcgg acggttcgcg gattcctgat gctcgcgccg
    34501 ccggctttcc tggccgcgat ggtcgcctac ctctctgccg ggcctcgggc cgcgacctcg
    34561 ttcaccgtct acccgttcct ggccgacgtc ctcggctcgg gcctcccgcg ctcgaacgtg
    34621 gcccgcttcg tcgtcgtcac gacccttttc ttcatcgtcc cctacctcgt ctcgggggtg
    34681 ctgctcctcc tggccgacat cggcacctcg gccgcagcgt ccctctggag acgggagcgg
    34741 cggaccccgg cggccgggcc acggccgcgg ctcctcgcgc ccgaggcgtt ctggacgctc
    34801 gtcggcgcct cggtccttct cgcggcggcg gccggctcgc agctcccgaa gctggcacac
    34861 ggcggcgagc tgccgggcgg cgtgaacgtc gcccccgtct tcgtcgccgc cgtgccgttc
    34921 gtcgcgctcg ccggcgccct cgtcctcgcg gcgatcgcct cgattccgag ggccgtcgct
    34981 cggcggttcg gcccccggca gcgttccgac ggcgggctat gatcgaggcg atgccgtctt
    35041 tcgacaccgt cgtcgtcctc gatttcggct cccagtacac ccaggtcatc gcccgccgga
    35101 ttcgcgaggc ccgggtccga tcgatcgtcc ttccgtactc cgcgaccgcc gcggagatcg
    35161 aggccctcgc gccgaaggga atcgtcctct ccggcgggcc ctcgagcgtc tacgacgaga
    35221 aggcgccgcg cggcgacgag aaggtcttcg acctcggcat ccccgttctc ggtctctgct
    35281 acggcatgca gctgatggcg ctccacttcg gcggcggcgt cggccacgcg ccggggcggg
    35341 agtacgggcg ggcggtcgtc gacatcgtgg gcgggcggct cctcggggcg ctcgggccgc
    35401 gggagaccgt ctggatgagt cacggcgacc acgtcgaggc cgtcccggac ggcttcaccg
    35461 tgacggcgcg gacggcgaac gctccggtcg cagccttcga ggacgcgcgt cgggcgctct
    35521 acggcctcca gttccacccc gaggtccacc acaccgacca cggcgccgag atgctcgagg
    35581 cgttcctcta cggcgtctgt ggctgcgctc cgacgtggac gatggcgagc ttccgcgact
    35641 cggccgtcgc gaaggtccgc gagacggtca ccgagggcgt cgtcctcggc gccctctccg
    35701 gcggcgtcga ctccaccgtc gccgccgtcc tcatccggga ggcggtgggg gagcgattcc
    35761 ggggggtctt cgtcgacacc ggcctcctcc ggaaggacga ggggcggcac gtcctcgcgg
    35821 ccttctctca cctcggcctc cccgtgacgg gcatcgacgc ttccgagcgc ttcctctccg
    35881 cgctcgcggg cgtgaccgac ccggagcgca agcggaagat catcggcggg ctcttcatcg
    35941 acgtcttcgc cgagaacgcg aaggccgtgg acggcgccgc gtggctcgcg caggggaccc
    36001 tctaccccga cgtgatcgag tcggtctcga tccacgggcc ttcggccgtc atcaagacgc
    36061 accacaacgt cggcggcctc ccggccaagc tcggattccg gctcgtcgag ccgctccgcg
    36121 agctcttcaa ggacgaggtc cgccgcctcg gcgaggagct cggaatcccg cgcgacatgc
    36181 tctaccgcca cccgttcccc gggccggggc tcgcggtgcg gatcccgggc gagatcacgc
    36241 ccgagcgtgt ccggatcctc caggaggcgg acgcgatctt catggaggag ctgcgggcct
    36301 cgggcgacta cgaccgcacg gcgcaggcct tcgccgtact tctgccggtc cgttccgtcg
    36361 gagtgatggg cgacggccgg acgtacgagc acgtcgtcgc cctccgcgcg gtgacgacga
    36421 tcgactacat gacggccgac tgggcccgcc tccctcacga cctcctcgac cgggcctcgc
    36481 gacgcatcgt cggcgaggtc cgcggcgtga accgcgtcgt ctacgacgtc acctccaagc
    36541 cgccggcgac gatcgagtgg gagtgagcct cgggggctct cccgtggctg cctccgggca
    36601 gcagttgaca accggtcgcg gccgtgttgt gctgaaaggg acccgttccg tctccgactg
    36661 gcggtgagga cggtgacaac tcgcttcccg tcggctcgga atcgcgccga ctcccaaaac
    36721 cgctcgcctc ggcgcgcact gaaacaagga cctcgagatc gaaatgaagc tttacgtcgg
    36781 aaacctcccc tacacgatgg atgacgccac ccttcgcggc ctgttcgagg gcttcggaga
    36841 ggtccactcc gcccgcgtcg tgagcgaccg cgattccggc cagtcgaagg gcttcggttt
    36901 cgtcgagatg aacgacagcg aggcccagag cgcgatgagc gccctgaacg gccaggagca
    36961 cggcggccgg acgctgcgcg tcaacgaggc ccgcccgcag gaagcccgca cgggcggtgg
    37021 cggcggtggc cgcggctacg gcggcggcgg tggctacggc ggtggtggct acggcggcgg
    37081 cggcggccgc ggccgctact aacccttccc ggtagtaccc caagatctac gggccgggta
    37141 gggcggagga gtccgccctg cccggtgctc gttttcgcgc aattgccgcg catgagggct
    37201 cgccgactcc ggactttccc atcctcggtt ctgccgacgg tcctcgcgat gaagctcggc
    37261 gtttgacgaa aaccgaacac cgggtgtgtg gtttattggc ggaagaagcc agaattccat
    37321 ccttgcgcaa ttccactatt tccacataga gtcccaggtc gcgtgccaag cggtttcgtt
    37381 tccttcgggg tgaccgcccc cggcgccgaa gacggtcgac gccggccggt ggccttcaaa
    37441 cgtctgttcc tgaccttcgc gatctcgctg ttcacccttc tcctcgcccg gggcgcgtcg
    37501 gcccagtcgg gcggagcgct tgcatccggt cagctcgcga ttcaggggac ccgcctgtcg
    37561 atcgccgccg gagacgagtc acagatcctc gacgtcgccg agtcggcgcg cgttcgcacc
    37621 tgcttcgcgg gtgtctgcgg gtcgatggct ccgggggatc cgcgcgtgaa cggcctcctg
    37681 gtgaaggccg agctctcggg ccccgagctc caggcgtcgg cgacctacac ggcgacgccg
    37741 ggaggagcgt tcctgctgcc gggggtccag acggagggaa cgtacgtcct ctcgaacatc
    37801 cgcctcgtcc gagcggcggt tccggacggc gcggacgagg aggtcctcgg tcaggcgact
    37861 ccggccgtcg tgaccctcga ggtccggcga atcctcgcga cgacggcgac ggtgaagcag
    37921 ctgaccctcg cagagctcca ggcgcggggc atccagatca cgcaacagaa ctacgacgcc
    37981 tacagtttcg ccgtcggttt cgcattccag ggcgggctcg tgacgatcga cttcccggtc
    38041 ctcttccagg acggcggacg ggtcgatctc ctctcgaaac cggaggtgaa gctcgacagc
    38101 ctgcccgagg ggctggctgc ggccgtgcgc cgttggcagc ccccgcacat cgtcccgttc
    38161 aagctggagg tgggcggacc gccgggaatc ttcccgaggg aggagatgga ggagctgcct
    38221 cccgagacga agctcttcgg cgtcatcgtc ctccccggga acgtctcgtt cctccacaag
    38281 tacttcgacg cccagctcct cgtcgcgaac ggcgcgccgg ccggaagcgg ggccgtcctt
    38341 tcgaacgtca cgagcacgat tcgcctgccg gccgccgtcc cccgcgagct gcttcggctc
    38401 gccgggacga caccgtccgt ggcacccggg caggccgtgc ccgtcctggg tctggcgggc
    38461 gagacgcagc tcggtcctgg cgagcagggg acggcgagct tcacgctgga aggggtcgcc
    38521 gccgggacgc acgtcgtcac gatggaggtc gccgcggacc tggagcggcc cggacgagac
    38581 gtccttccgc tctcggggaa gctcaaggcg gcgatcgagg tcgtcgacgc ccgcttcaac
    38641 ctcgcgttca gtcacccgga tgtcgtccgg gcggacgagg cgtacacgct cttcgtcacc
    38701 gtcacgaacg tctcgacgcg cgactgggag aacgccatca ccctcgcgct cgatgccgcg
    38761 caggcgaccg gggccgtcaa ggcggacccg aacgacacgt tccagcgaca gatcgagacg
    38821 ctcgggcccg gcgagggcgc gacggtcgag ttccggctcg tcgcgaaggt gacgggacgg
    38881 tgcgtcgcga cggcgttcca gtcctccggc tccgtccagg gggtcatccg gctccggacg
    38941 ggcgtcggcg agcacgggat cccgctatcg ccctcctctc tcgtcctgcc gaggttcacc
    39001 ggggagctgc ccgagccgct cgtcgcggcg aacgtccccc tcctcggcct cgcctacagt
    39061 ctcgccgtcg cgcctccggg cgcgattccg cctgacctgc cgcgactcgt gaagtccgac
    39121 gtcgagcgga gagccatcga cctcgcggag gccggccagc gcatcttcat cggcgaggag
    39181 cttctcccgt cgctcgaggc gctcctcctc gatcagctcg ggaaccgtca cgaccggccg
    39241 gagttcgacc agcttcgaag gactctcgac aagggacgga cctcggccga ggggctcgcc
    39301 tcttacctca ggcaggagca ggaggcgcgc aacctcgcgg cgtcagcgct tctcgagcac
    39361 atcgccgaca cgatgagcta cgcgcggccc tggctcgccg ccaccgtcga aagcgcggga
    39421 tcgggggtcg ccccgacgct cgaggtccgc aagctcaatg gcgtcaccgg cgtgacctac
    39481 ctcgcgtacc cggcgggcga cccgagggcc cttcgttccc tggcgttcgg cgagacgtac
    39541 gacatcctcg accgtcccgg agggacacgc gctccgctct ccgtcgtggg gcggctctcg
    39601 gcggaagccg ggtattcggt cgtcatccac ggaccgatct ccggcgagtc gatgcccgtc
    39661 accctctcct tcgtcgcccc ctcgtcgtcg ggcggcggct tctcccacgt gcgcttcccc
    39721 gcgttcgcgg tccccccggg ccaagcctgg gtggtggatg tcgtccgcgc ggagggcgac
    39781 acccggccgc tcgcgttcga tcttcgtcac ctcgcgacgg gcgctccggt ggccggtgca
    39841 ccggtcgccg cggtcagcgc cgtcgaacgt ccgccgttcc gactcatcgg ggcgcgccag
    39901 gacttcgtcc tggatccgta cggatcgggc gtctggtacc tcttcaaccg cccgccggcg
    39961 aaggccgacg ccgaagcggc ggaccggtac gaagtccgga cgagcttccg cggcctcgac
    40021 acgacgagct cgggtacgac gctggagcgt tccacggtct tctccgccgc gtcggcgctc
    40081 cttcagcccg cgagcgaacg agtcgtcgcc gttcggttct ctcagcccgt ctcggcgatc
    40141 agggggttgc acgacgatca gccggtcatc acgcacgagc acgaaatcga cctcgcgggc
    40201 ctccgtgacg gctggggtga ctcgctcgtc ggccccgtcc cggacatcgc gatcgagccg
    40261 gaccacgtcg gcgggctcgt cgaggggaag gtcctgaggg ggacgggcga agccgtcgcg
    40321 ggagctgtcg ttcgtctcgt tcgggatcgc gtcatcgtga tggacggcgg cgccgatgcc
    40381 agagtcctgc acgacctcgt cgcggagcga acgaccgacg actcgggcgg gttcttcttc
    40441 cccttcatcg aggaaccgat cctcggggtc ccgaggccgg ccgagaaggg ggccgtccag
    40501 tccgggttcc ggattcaagc cgtcgtacct gccggcccgg atcccctcgc gccggaggag
    40561 cgcgaggagg tcacgtcgat catccgcctc cagtcgcgcc tcgcccgcgt caacatcgcc
    40621 atgctcggcc ggggcacgat cacgggccag gtcctctacg aagactcgtc tcccgttccc
    40681 gcagcggccg tcacggcggc gagcaccctt ttctcggaag tgcgctcgac ggtgagcggc
    40741 gccgacggaa cgttccggct ggaggcgatg ccggtcggac cgatcacggt caccgcccgg
    40801 gacgccgcgg gccgttcgtc ctacgcgacc gtcgggctct cggggccggg cgcgaccgcg
    40861 gacgtcactc ttcggcttca gcgggcggag gcgccacgaa cgggaaccgt ctccggccgc
    40921 gtcctcgttc ggcgcgagac ggccgcgggg ccggtcaccg agccggcctc gggcgcgtcc
    40981 gtcgcggtct actccgccgg ggggccgttc ggcgttgcga cgaccggggc gagcggggcg
    41041 tttctcttcg atggcgttcc ggccggtcgg gtgaccgtcc aggccgccga cttctcggtc
    41101 tcgcgcacgc cggcgctcgc cgtcacggat ctcgcgcccg acacgacgga gaacgtcgtc
    41161 ctcactctcg ccgtgtcgag cccgcgctcc gtcagcggcg aggtgcgcta tcgggacccg
    41221 gtcacccaga tcgacgggcc cgtcatcggg gcgaccgtct tcgtcgaggg gccgggcagc
    41281 ttcgccctga ccgacgagac aggcacctat cggatcgacg gcgtcccggc acagggaatc
    41341 aacgaggccc cgtacacggt ccacgcgatc gacttcgccc ggaagctcga gggaaggacg
    41401 accgtctcgg tgacggagac gtcgcccgac ccggtcgtcg ccgcgacgat ccagatcaaa
    41461 ggaactgcgg tgggctccat cgacggggtc gtcctcgatc cgctcggcca gcccgcggcc
    41521 ggggtcaccg tcacccttct gccgatcggc gagacgacga gcggcgccga cgggacgttc
    41581 tcgttcgagg gagtccccgc ccgacgttac accgtcgcgg cgcacagagg agatggcctc
    41641 cagccgggcg cgatcgggtg gatcggcgag gcgacgacgg aggtcctctt cggcgggcac
    41701 cgggcgttcg cgacgatccg gctccgcggt gccgggaccg ttcgcgtgag gacgctgacg
    41761 gcgaacggcc cggtccggac ccccgtttcg tacaagccga cgtggttcag cccgatggag
    41821 ctgaggattc ggcagaaggc caccgcgatc gagacgacgt ccgacgacaa cggaagcctg
    41881 gagctcgtcg ttcccgtcgg cgaactgagt gtcggcgccg cgagcccgtt ctacggaacg
    41941 gtttcgtacc aggggacgat cgacttcgcg ggacaggtca aggaggtcga cctcgtcttc
    42001 cagccgctgt cgaccgtcaa ggggaccgtc gtcgacgtcg acggagtgac ccccgtcgcc
    42061 ggcgtcacga tcgagctccg cgcacacggg ctccccccgc agcccaaggg gaccggacag
    42121 aacggcgagt tcgagttcca gctcgttccg agaggcccgc tttcgctcac ggcgagcgga
    42181 atcgtgggga cgatcgagcg tacgggccga acccacgcga tggtcacggg gccgggccag
    42241 gaactcgacg tcgtcgtccg cctgaaggcc aaggggaccg tccgcggtgg cgtcgtcgac
    42301 gagacggcgc cgggcgtcta ctcgccggtg gcgtgggcgc agtttgccct tcaggagaac
    42361 gacttcccct tccgtcgcct gccggcgtcc ccgggttact tcaaagcgga tgcgaacgga
    42421 cggttcgcgg tcgcggacgt cacggcggga cgcttcaccg tcgtcgcgcg ccaccccgag
    42481 caggtcacgc ggcagggacg tctctccggc gaggtgaacg ccgacttcga ggtcgtcgag
    42541 gctgggaaca tcgttctgtc cgggatggtg agagacctca ccgtcctcgt ccgcgacccg
    42601 gagactggct cccccgttcc cgacgcacag gtcacgctct cgaacggcga ggcggccgtc
    42661 gcgggggcgg atggacgggc ctcgttcgtc gcccttccca tcaggaaatg ggacgtgtat
    42721 gcgttccacg cgccgaccgg aagcggaggg cggctcgaca acatagacct cgccacggcg
    42781 ggcgaggccc tcgaggtcgt cgtcgtcctc gaccagaagg ggcgcgtttt cgggacccta
    42841 tacgacgacg cggggaagtc gactccggtc ggcggaggga ccgtgcgtct cgagggcacc
    42901 gtgaacgggc ggctctgggg ctcgcgagta acggctctcg cgacgacgag cttggaggcc
    42961 gaatcgctcg gggagttcgc cttcgacggg ctcccggtcg ggaactactc cttcaccgcg
    43021 gccgtcgagg ggagcgaccg gcgtgccgaa ggggcggcga ccctcacgcc gacggagccg
    43081 gagcagagcc tcgcgctcgt cctcgacgcg gtccgggagg tctatgtccg ggtctaccag
    43141 aaggtgcagg cgggggtact cgtcgaagtg aaccccgatc cggcacaggg agacggtgtt
    43201 ttcgccgttt cctatggacg tggttcgagc gccctcgcgg ggtcactgat cgcgagccgg
    43261 cttctccctg atcggccgta cccgaatcac cactatcggt tcaatgaggt cctggccaac
    43321 aagtcgttct ggattcaggt cgaagagtcg tccggagaga ggcgacgcgt cctcgtcagg
    43381 tcaaccgcgc tcagcggagg cgagttcgga gccggcaccg cggccgaccc ctacaggatc
    43441 gtcctcaagg cgcgaggcac cgtccgggtc accgtccgcg atgcggacgg agttgcggcg
    43501 gcaagcgcca ccgtgcgggt gcacgccgcg aacggcagct gggcgagcgc cgcggactcc
    43561 gacggcaggg cggcgttcac cgccgtggag gaaggggtcg tctccgtctc agcctcgatc
    43621 ccggattcgt ccagtggggc ctctgcctcg cgaaccctcg agtgggacga ccaggtcctc
    43681 gatttcgacc tgacgctctc gccagtcgtt tccgcgcacg gcgtcgtcta cgaggcgccc
    43741 gagaacgact cgtgggacgg cgatttctcg aaactgacgc cggccgaggg cgtctcggtc
    43801 cggatccagc cttcagcggg cgacgcccag ctgctcatca cgggttcgga cggcgcgtac
    43861 cgcttttcgg gcctcgcacc gggcggctac acgatcgtgg cgcagagcct cgacggatcg
    43921 cggatcgtcc gggcagccgg tactctcgga gacgactacg gggaggacta cctggtcccg
    43981 cccctgatcc tcgacggctc gcgtcctcgg atcctcaccc tgtcacctcc aaacggcgcg
    44041 tcgtccgtct cgcggaccgc ccgggtcgag atcctcttct ccgagcccct tctcccggcg
    44101 gtccttccgt ccggcatgac ctcgtcccac ttccacctga ccttcggcga gaccccggcg
    44161 gcaggaacgt ggtcctcctc cctcgacgac caagggaggc aggtcgtcca gttcacgccg
    44221 acgcccgagt acgagagctc gacgacctac tccctgcgaa tctcgggcgg cctctcgggc
    44281 gtccaggacc gcgaggggcg tcccctcacc gacagcggtg acatcggcgc gagcttcacg
    44341 acctcggaca ccgttgggcc gcgattcgtc aggacggtcc cgtcgttcgc gcggcccgtc
    44401 gacccggaga cgccgatccg gatcgacttc ggcgaggtcc tcgtcctcac cacggagcag
    44461 ctccaggccg cggtcctctt cgagtggaag aacaccgccg gcgcctggac cgtccacccg
    44521 gtcgaagtct cgctcacgcg agggggctac tcgatcctcg tcaagcagcc cgtcggcgtc
    44581 accttcacgg acgactcgct gcggcgacgg ctcaccgtca gcggtctgac ggatgcgctc
    44641 gggaactcga tgctgccgtg gtccgaggag taccgcatct acgacgagaa cccgccgatc
    44701 ctttcgatcg accctccgcc gaacgcaccg tccggagtcc tgatcgcctc ggtgtcctac
    44761 accctcttcc cggagttcgc cgccctcgac gacgctactc cagagaacga attcggcgac
    44821 atcgatcggg tcgaatacac cttcgcgagc gcggcggacc cggagcagcc ggccccctcg
    44881 cccggtgccg tcctcacggc ggcgccgttc gcgttcggct tcgtcgcctc ctacgtcggc
    44941 aacggctcgg attcccggcc cttccccgtc tggttgaagg ccttcgacac gagcggaaac
    45001 gcgagcgcgc aggtccgcct cgacatgagc gtcctcccgc ccaggtcccc cgtcgtgtcg
    45061 ggggtgacgg tcttcgcgac ggctccggtc gccggcacca cctatgccgg ctcgactctc
    45121 accgccacgg cgatcggcct ctccgacgac gacgacgcgc gcctcacgat ctccgcggag
    45181 ttgcggcgcg ggaccgccga ttcgagcgag ctcgtcacgt cagctcccgc tcgatccgtc
    45241 gatcggccgg cggagggatg gagcgcgctg ccggcgcaga gcttcacgct cacgctcccg
    45301 gtcaccgagg ccgaaggcac ggcgttcatc gtccgggtcc gggccgtgga ttcgaagggg
    45361 ggcgttggag aggcggatgc ggcgttcgtc gtcgcggacg atgccctcga tccggtcgtg
    45421 acgaacctcg tcgcgagacg cccgaatggc gacgccgcga cctccttcgt catcggcgag
    45481 acgttcgttt tcgaggtcac cgtccgcgac ggggagaccg gggtgaagtc gctcaccgtc
    45541 gcgaaccccg gaggggtcct cccggcgacg ctcgcggcga cccgcgtcga gacttccgac
    45601 ctcttccgga ccgagtcggt gacggtcccg gcctcgctca cggaggccat ccaggtcgaa
    45661 gtccgtgcga cagcagagga cttcggcggg aatgacgcca tcaccaccct ttcgctcgac
    45721 ctcgcaccga gcagcgaccc gacggatcct gcgatcgagt ggctctcgcc cttcgaaggc
    45781 ggcctctggc ccgcgaacta cagctccgtg gatccggcga aagccggcgt cgacctcctc
    45841 ctccgcttcc gcgtgaccga caaggacgtc gacgggagcg ggaacgaggt cccgggctcg
    45901 gtcgcaagcg ttcgcctccg tggaccggtc gacgcgacgg gagccctttc ctccgactgg
    45961 acgagcgccg cgatgctgac gcagggtgag ggggagtgga tctacgaagg ggtctggcgg
    46021 gtgccgaacg gagtcgccgc agggacggtg ctccgattcg aggccgaggc ggtcgacgcg
    46081 ggtgcgaaca acacgacgaa gtatgtcgga gtccaggcga tcgccgtgcg gcacgtctac
    46141 gaggcagcga cgaccgcggt cggctccggc tcggagattc ctgccccgac cggaaccgaa
    46201 gctcttccca tcttcctcct cgacggcacg actctcagcc tctacccgcg aagcgcgggc
    46261 ggcccccgct cgtacgcaaa cctctatctc tactcggggg gggccctctc ggcaggcgcc
    46321 gggtcgccgg tttcggtccg gagaacgacg ctgacctctc cggaggtcac gtcgtacgag
    46381 tcggctgtcc cttactaccc gctcgagctc tcgatcgggg agacgctcgc gatcggacac
    46441 ggagcggcga tcgacgtctc gggacgcggg cttctcgggg gcgacggctc ccacagcccc
    46501 gtcgtgctct ccggcgagcg tgcctcgctg cccggggccg cgggttctca cggggggctg
    46561 gggcgtcctc cgcaagggga ttggcttcag cgtctcgagg atccgggaag cgtgtacggc
    46621 agcgtccgcg agccgcgact gccgggtggc ggcggcggga accgcggtgc cacgggtggt
    46681 ggcgtcgtcc ggatcgaggc cccgtcggcg acggtgcgcc tccatggcaa ccttctggcc
    46741 gatggggggc cggtccctgg cggaggcacc gtcggcgccg cgggaggctc gatccgactt
    46801 cgagcgggaa cgctcgaggg ccgaggcgag gtacgggcga gcggctcctt cgtcgccgcg
    46861 ggtctctacc ggccgagcgg cagcggcggg cgtgtctcgc tctcctgggt cgagcccacg
    46921 atcaccggta tcaccgctcg ggcggacggc gccatctcgt ccgcctcctc cggtccgggc
    46981 agccccggca ttccgccgct cgcgggcgcc ggtaccgtct tcctcgaacg actcgactcg
    47041 gccggccagc cgttagaccg cgggacgctc cggacctcga acccgtcctc gaccgatctg
    47101 ccggcctgga cacggctccc tggcctcggt agcgctgtcg tcttgggagt cgacgccacg
    47161 agcaaggagc tcgtcgtttc cgcgcccgcc gcggagggct cgatcgaggg agaacgtatc
    47221 gtcgtcacga tcccggccct ggcgggtggg aatccgacgg tgctcgtcct gccggtcacg
    47281 gcgcagacgc ggaccgcagg ctcggtcggg tcggctgacc agtcgttccg tcttgccgtc
    47341 gctgcctcgg agcccgaagt ggcgcagatc gcgtcggcgg tctcctcggg tacggcggtc
    47401 acggcgcggg ccaggctcag ctacgcggca ttcgaggctc gtggggccgc cagggtcgtc
    47461 tcgagcgacc agctcgaggt cggcggcgtc gtcgacgacc gaacggcgat gtcgctcgat
    47521 ccgtcggcgc gcatcctctt cggaggcgag gcgccgacgg tttcgttcga ggggacgagc
    47581 ccggctcccg gggtgaacgt gacgcagggg acggcgatcg acgtccgctt ccgcgtcgcg
    47641 gaccctctgg gcctttcgaa gctcgaggag acctggcttg acgcggggac gaccgcgacg
    47701 cggacgttct cggagcctct cgaggcgttc gccgatccga cgccgacgcg agtcacggtg
    47761 ccggtgtcgc agccggccgg tcccgtgacg tatcgcgttc gagcgatcga ccgcgcggga
    47821 agggcgacgg agtccgtggc gacctggaac gtcgtcggag atgcgaccga ccccgtcatc
    47881 acctcgatcg cgctctcacc cgtgaagacc gaagacgtct acgcggcggg cgacaagatc
    47941 acgatcaagg cgtacgcgac ggacgacgtg aagctcgccc gaatccgaat ctcggtggac
    48001 gggcacacga tcgagtccgc gacgtcgccg gccacgctcg tctggctcgc gccaccggtg
    48061 acgacgtcga cggcctacga cgtgtccgtc gtcgccaccg acctgacggg caacgctgcg
    48121 actggcggca aggtgatcac ggtgacaccg cggatcgacg cggtctcgcc gatcgtcgcg
    48181 atcgactgcc cctcgtccgg agcgatcctc ccatccggct acggcaactt cgtcctccgg
    48241 gcgacggcgt cggatgacct gggagtacac cgtgtagatg ccttccgcga gggtgagaca
    48301 accccgttcg cgaccgccta cgccgcctcg ggatcggtga aggccttcga ctccagcatg
    48361 ccggcgatca cgctgccgag cgtctcggaa ccgacgctgg tccgcttccg ggtacgggcc
    48421 gtcgactttg gggggaacgt gtcgtcggag gtccaaacgg agatctccgt cattccagcc
    48481 gtccggatca cagcgacaga ttccatcacc tggtcgacgc tggtcggcga gacggtgtac
    48541 ctcgagtccg gaacgctcgt catcgacgaa cccgtcgcgc tgggtggcct gctggtcctg
    48601 cgtggcgcct ctgtgaccca ctcggcgggg ggaaccaagc gcctcgacct caccgtgagc
    48661 ggtccgctct tcgtggaatg cggcgggtcg atcgacgtga cggggaaggg gtatccggcg
    48721 agggcgacgt actcggggaa tgtcgagtcg acggactcag gaggaagcca cctgggagtg
    48781 ggaggggtgt ccgggagtgc ggcgaaggct ctgacctacg ggagcgtgga gcgaccgcgg
    48841 gaggctggga gcggcgcgtg gggaaccaat ccgaggagtg gcggcggagt cgtgaggatc
    48901 ggagcgggat cgatcgtgct tggaggctcg atggcatcga tcctggcgaa cggaggactg
    48961 ggcggatggg aaggcggtgc gggtgggtcg atctggatca cggcagggac gctaacgggt
    49021 gatgggctga tcgaggcggc ggggaacgat agcgcccagg atggtggtgg tggcgccgtg
    49081 gcgatcgagt acgggagcgt gagtggcacg gtgctgacgc gagtcaacgc gagaggcggc
    49141 ggaaccggaa gctccgcgga gaacggagga gcgggcacgg tggtcctgaa gggtcccggg
    49201 gcgacgtacg ggacgcttcg agtggacaac aagggaacga gcggacaggc gacggtgctg
    49261 ccgagcctgg ggtctggggt ggcgcaggac gggacgagcg gagcgacgct cgtgacgcag
    49321 ctgacgtcga ttccggcgta cttcgcaggt cactgggtgg aggtgcggcg aggcgagagc
    49381 ctgctgggga cgtggcggat caagacgatc actggcggga cggtgacgct cgagccgaac
    49441 gaggcggagg cgatcgcgct gcaggcggga gatgggtggc agggcgtcta ccgctttgac
    49501 cgggtggaag tggccgggaa cgccgtcgtt cagagcggcg atccgattcg gactccgacg
    49561 gtggagctga ccggtcctac gggaacgggt ggcaacacgg aggctccggg gccggtggtg
    49621 gcggatacgg tcgtcgtgag cgggaacgtc ctgacgtcgg ggatcgaagc gaagacgttg
    49681 acggtgaagg cgggggcgag gctatcgtcg ccggcgtcga cagccacgga ggtgaagggc
    49741 cttgagctga gcatctcggg cgtgctgacg atcgaggacg gcgggtcgat cgacgtgacg
    49801 gggaaggggt atccggcgag ggcgacgtac tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49921 nnnnnnnnnn nnnctggcgg gacggtgacg ctcgagccga acgaggcgga ggcgatcgcg
    49981 ctgcaggcag gggatgcgtg gcagggcgtc taccggttcg atcgggtcga ggtgaccggc
    50041 aacgcggtcc ttcagagcct ggacaccgtc tacgacggac tgccggagat cacagtctcc
    50101 tcgccggcgc cgaacgcgac gttcgcctcc ggagccaccg tgccggtctc gttccagacc
    50161 gccgacgacc gaggcgtcgc gaaggtcatc ctgtcgctcg gtgaatcgag agtcgagatc
    50221 ctcgggtccg cgcagacctc gggtgtcgtg aaggccccat gggtcggaac gacgaccacc
    50281 atgcctctcg aggcgaccgt cgtcgacacc gtcgggcaga gcaccacgag atttgtgccg
    50341 ctcacgatcc tcgcggcgcc gtccctcacg gcatcgatca gctacccctg ggacggcgcg
    50401 aggctctggg ctggaacgac ccaggaggtc agcgtgcagg ttccggatgc ccgaccgatc
    50461 cgtagtgtga cgatcgggct tgggacgttc tcgatgcact tcgacagtgt cgttgggccg
    50521 accctcgacg tctcgattcc gatcccgccg gtggcggtcg acgaggacag gttgctcacc
    50581 gcgacggtcg acgatgggct gggagcaccc gtaacgtccc cggccgttcc cgtcactctt
    50641 ctcgtcgaca acggtccgag ggtgacggcc tggacggagg caatctcccc tgtcaacgcc
    50701 ggatccgacg ttcaggtcga cttcagcgcc ttcttccgga gctgggaggg ggcgcagctg
    50761 agacttcagg tcacaggctc cgtaagcctc gacgagacat acgacgcggg gcagttcggg
    50821 agcggcggtg ccggtggtgt ggccgccctt gatgttgccg attccattag ctggtcattc
    50881 ccctccttcc agatccctac cgatgctcgc ggaccgatga ggatcgtggt gaccgccatc
    50941 gatggcgccg gccggatggc atcggccgat ccgatcgacc tcgaggtcac cagcccgctc
    51001 tcaatctccg tcgtcggctc cgggaccgtc tctccaggcg gcacgctcga gctcgccctg
    51061 agcgcgaccg gctggaacct cccggagagc gtcgatctcg agatccaggg ggcgtttggc
    51121 tggtcacgtc cggtctcact cggggagagc tacggcgaga gcgagacggt ctctcggacg
    51181 atccgaatcc cggtgcctcg gaatgctctc ggcgaagggg ccgcggcatt cgaggcatac
    51241 atggacggag gcgagggcgg ctactccaga ggagccctcg cggcgaagct cgatgagggg
    51301 atggcctcct cgaacacggt aaccttctcc gttgtcgacg atgtcgaccc ggtcgtcgcc
    51361 agggtgcgtt cgtcggagtg gggcctggtt tccggcgcag aggcctcgtt ccgtgccgac
    51421 gtctccgaca acggcaccgt tcaggaggtt cggttcctcg tggatgacgt gctcatcgcg
    51481 acggtttccg agccgacgga gctgtcgacc tacgattcgg ggccgtatct ccttccgacc
    51541 gcggcggagg agcgcagcgt cgtcgttcgg gtcgaggcga ccgacctcgc cggccggagc
    51601 agcagcgccg tacgcagctt cgtcctggcc cctaccggaa cgccgctcgt acggttccgt
    51661 gcgccgacgt ccggtgcgat ggccgtcgcc ggccaaacgc ttccgctgtt ggccgagctg
    51721 aggcattcgc ggctcatcac tcaggtggcc ttctaccgcg acgacgatgc gactcccttc
    51781 gcgacgagaa gcggggaaga gctcacggcg gagttccccg ttccgccagg tgcgacgccg
    51841 ggaagcgaga tcacgctctg ggtcgaggcg acggacgatc tcggtgccgt cggggaggct
    51901 tccgcggtcg tgagaatcgt cgccggagcc ctgctgacgg acgggaccat cctcgatgcg
    51961 gacgacgcat cgcacgagga cgagacgatc gtcgtccagg gccgcgtcga ggtgcggggc
    52021 gagcatcggt tcgctcggct ggtcgtcctc ccgggtgccg ttctcacgca cgcggcgacg
    52081 gctggagaga cgatcgagcg cctcgacctc gaggtgacgg gcgacgtcta tgtttctccg
    52141 tcggcgacga tcgatgcgat cggcctcgga ttcgaaggag gactccagcg atcgaacccg
    52201 aatcccgaag gcaggacggc acctggcctt caggaagcgc aggccggact cggggggagc
    52261 catgccggcg agggagagcc agccgaggac gggtggggcg cggagaccta cggtagctgt
    52321 cgggccccgg atctccctgg cggcggcggc ggcgcctcgc tgagcgggga tcccgggggg
    52381 tcgggcggcg gcgtcatcaa gctccacgtt ggtggccggc tgatcatcga cggggcgatc
    52441 gacgcgtccg gaatctccgg cgagggcgga gccgcaggtg ccggcgggag cgtccagctg
    52501 gaagcgacct cgatcggcgg taccgggtgg gtccgcgcga tgggaggcga tcccggtgcg
    52561 ctcgtcggac ggggaggaag cgccggggga ggccgcatcg ccgtcctcgg gccgctcgag
    52621 gatgcggccc cggagctgag cagcgcgagc ggtcccgcgt ggtacgcgtc cggaggcgcg
    52681 ggcacgctgc tcctcaggtc gcccgaggat ctccacggcc acctcattgt cgactccgga
    52741 gcatcgtccc gggtgggctg gacgtacctg gccgaggtcg gttggcgctg gggcgacctg
    52801 gccggggagg acgatctcgt cgtgaggatc gatcccttcc cggtagacgt cgatctcgtc
    52861 gggaggtcga tcgagatcac gcgagacaac gcgctcgtcg gccggttccg tgtcgaggag
    52921 agccggccgc aagagggctc ggtgaagctc gacgagagcg cggcgcgctt gctcccatcg
    52981 tgggggttcg atattcgcgg cgtgcaggac ttcgacaccg tgagcgtgaa gggaagagcg
    53041 gcactcgatg tctctgaggt cctcgagagc atcgtgcggc tcggtccggc cgcgtggatg
    53101 tggacggaag gcgactacct atgcccgggc gaccccgcgc catggctgtc gactggcacc
    53161 ctgagcgttg aggcatggcc cgggggcaac gtcgtgatcg acgtcgagag cgacgactcc
    53221 aactactgct ctgggggcga agtcccgggc gcggtcgtcc agtactcggt ctggatcgac
    53281 ggcgtggagg gctcgctcct ggaggtgccg atcgagggcg cgccggcaga ggacgcccgc
    53341 cagctcacct acgccctacc ggcttcgctt ccgctcggcg agcacctcct cgtcgtcgaa
    53401 gtccgagacc agtcgaacca gaagaccgcg atcgccttcc ggatccgtgt cgcggaggct
    53461 cccggactcg tgcagcgtct cccggccccg tccgggggac ggtcccatcc catctcgctt
    53521 cgtcccgcga agcgatttgc ccgtccggcg cgtgaagccg ccggccccgt ccgcactcgc
    53581 ggggcggggc gtgggccaca ggagcctcat tgaatctctt ccgttccatg ctcccgttgg
    53641 ccgtcctcgt cgtccccgtc gtcgcgaaag cccagccctc cgaacccttc ccggccccgt
    53701 tcgtcatcga gcaccacctc gtcgacgcga gcctggaggg gacgcccgtg acgggtgaga
    53761 cggtgcgcga cacctacggt gggagcttcc tcgtctcgga gaggccggac gggaaccgga
    53821 ccatcgtcga cttcgcccgg cgtgagctga ccgaagtgcg gccgacggag ggcacctact
    53881 ggtccctttc gttcgggcgc ttcgcggagc tccggaagag actcgtagac gcggagaaga
    53941 ccgagacgac tccagaggtc cggactcagg cggcgcgcga gaagcgtccc gtgcgcatcg
    54001 aagaggtgcc ggacggggcg gaggggaagg tgcgaggcgc gctctcggcg acgggcagtg
    54061 caggacggcg ccttcgcgcc tccgtcgaag gtggatcagc cctcgacgtc tggctggacg
    54121 tctcggcgcc gcgcctcacg gcagcgggcg tcgccgcgct cgcggacttc gagcgaaacg
    54181 tcctgggcgc ggccgacggc gaggtcggcc ccgccgacct gctcgaagcc gttcgtctga
    54241 aggaaggcgg cgccctcgcc gtcaggaccc gaagaggagt gggccgcgcc gaggccttac
    54301 gggacgatgg gccctacctc gaaacggtcg tcacgaaggc gatgccggtg gcggccctgc
    54361 ccgcgagcct cgtctcggtg ccggaggggc tccggcgcgt tccgagccct ctcgaggtcg
    54421 tcgtctcctg ggccgaagac gaggcggcac ttcggcagcg aggccggaag tgaagatgtc
    54481 ccggaacagg ctcgcgccgc tggcgctcgc gacggccgtg accctcggtg tgctgccggc
    54541 gcatgcgcag caggacccgt tcgccctccc ggcgagcttc ctgacgcagg ccgcgttctc
    54601 ctgcggcaac gtggcagtga ccggcagcgc cgtcgtcacc tcggccggcg tgggcggcgc
    54661 gggccatgtc gtctcgaacg ggaacatcac gctcaacgga agcgcgaaga tccgcggtaa
    54721 cgcgaccgcg ggccccggga agaccatcaa gacgaccggg tcttcgcaga tcacggggac
    54781 gcggtcgtac ctgtcggagg cctggccctg ctctcccatc gacctcgcgg cgctcggcca
    54841 gactctcgcg acggcgaacg acaacgcgct cgtcccgaag acgagcagcg gaaagaaccc
    54901 cctgtcgggc tcgagcccgc ccgatctcac gctgaacggg agcgacacgc tcgtcctgcc
    54961 ggccgggacg tactacctcg ggaagatcac gatcggcggc agctccgtcc tctccgtgtc
    55021 cggagacgtg cggatccttg cgacggggaa ggtgtcgatc tcgggttcgt cgaaggtcaa
    55081 caacgccggg agtccgatcg ctttccggct cttcacgagc gggtcgccgt acgcgctcga
    55141 ggggagcgcc cggctgaagg gcttcgtcta cgcatcgagc gcgacggccg tggtgaaggt
    55201 caccggcagc ggcgtcttcg tcggagggct ctacggtggc cagctcacgg tcgaggggag
    55261 tgcctcgctg acccgcgacg tcgtgtacgt cccgcctgcc gacccgctcg tcctctcgat
    55321 caccgagtcg ggccagccgc tcgcggaagg agcggtcttc ggccgggccg tcacccccgt
    55381 cgtctcgacg acgggcggag tcgcgcccgt caccgtttcc gcgaccctcg atggcgccgc
    55441 gtggacgtcg gggaccgtcg tctccgcgaa cggggagcac acgctcgccg tcgccgtcac
    55501 ggactccagt gtgcccccga cccagctctc ggagacgcgg cacttcacga tcgacaccga
    55561 agggccctct ctcgtcatcg cgtcgccgcc tccgggcgcc gtcgtctcca cctcgccgat
    55621 cgtcgtgacg gggaccgcga gcggggcgac gtccctcacc ctgctcggcg cgcagatcgt
    55681 gctccaggag ggaggggtgt tctccgcgac ggtcccgctc gtcgaaggga cgaacatcct
    55741 tcgtctcgtc gctcgcgacg ctgcggggaa cgagacgatc cgcgacctcc cggtcgtcct
    55801 cgacacgctt cccccggtcg tcaccctttc ggcgccctcg tccgggacct gcctcgcggc
    55861 aggaacaccg ctcgcggtct cgggccgtta cctcgacgcc catccgcggc ctgcgggagc
    55921 cccggagggg cccgcggtca gcctcacgct gactctgccg tccggtgcgg cgacgacgct
    55981 ccccgcgagc gtcgacgaga gcggagcctt cacgggctcg ttcccgattc cggatggcac
    56041 cgaagggagc gcaacccttc tcgcgatcgc gacggatgct ctcggaagcg tctcgagagt
    56101 cctctcctcg gtgaggttcg actcggcgtc ccccgaggtc tcgatgacct ccgacgggac
    56161 ccccttcccg ggctcggggc ctggcgccac gccccctcag ggggcgacgc ccgctttcgt
    56221 caaccgcccc ctcgccgccc gcgcgctcgt gcgcgacggg gccgccgcgc cgcccgccgc
    56281 gactctgacg ctcgacggcc agccgtacac agaggggacg ccgatcgccg ccgaggggaa
    56341 tcacctcctc gtcgcgcggg cgaccgactg cgcgggacac gagtccgcgg cgcacgcctt
    56401 cttcgccgtc gacacgacgg cgccggcgct tctcgccacc gtccccgccg agaacgccct
    56461 cctcaaggaa ccggtcacga ccttcagcgg gaccgcctcg gcggacgtcg tcacggtcac
    56521 ggtcggcggc cagagcgtca ccccgacccc tggcgccgac tccacgacgt tttctctggc
    56581 tccctacgcg tggcgcgagg gcgacaacac cgtcgtcgtc gagctcgtcg accgcgccgg
    56641 gaaccgcgcc tccttcaccc gggccttcca gataaagacg accggtcccg tcgtcgagat
    56701 cctcctcggg ggcgtgccgc tcggcacggg gaagaccttc ttcgcgccga taacgcccga
    56761 catccggacg aacgaaccgc tcagcgggcc tggagcggcg acgctgacgg tcaccctcga
    56821 cggagcgccg tacgtccccg ggactccgat cgcggtggcc ggtccccaca ctctcaaggc
    56881 gagcgtcgtc gacgcggccg ggacgccggc gttcgacacc gcgtctttca cgatcgacac
    56941 ctccggcggc ccgagcgtct ccatcacgtc gcccgtcgac ggagcgacgc tccccggccc
    57001 gacggtcgac gtctccggca ccgtctcggc ggcgcacgcg gccggccgga cgatcgtccg
    57061 ggtgaacggc cgcgaggcga cggtccccga cgccggcgtc acgtggactc ttcccggcct
    57121 gcctctcgag cccgacgcgc cgaacgacat cgtcgcggtg gcggtcgacg ccctcggccg
    57181 atccgcctcg gcggccgttc aggtgctcgt ccgctccgac ggcccgaagc tcgtcctcat
    57241 ctcgccggca gaggggacgc gaaccaatcg gaggaagacc gacgtcgtcg gcgcggtcgt
    57301 cggcggcgcc tccgccacgg cggacggact cgtccacgcg ggcgccctct cgacgacgat
    57361 cgacgccacc ggctccttcc gccttctcga cgtcccgctc gccgacggac cgaacccgat
    57421 ctccgttacc gccgtggacg cccagggacg gaccggcgaa gcgcacgtca ccgtcgtctc
    57481 cgacaccacg gcacctaccg tctccttcac ggtcagcggt cagccgctcg aagagggggc
    57541 gacattcgcc ggtccgatga cggttaccgt gtcggtctcc gacggggccg gcgacgcgcc
    57601 ggccccgcgc atcctcctga acggcgcggt ggttccctcg gcgaccgaga cgacggaggt
    57661 cgcgatcccg gatgagggcg gatgggtcct ctcggtcgtc gccctcgacg gcgcgggcaa
    57721 cgagacccgg gcgagccgca gcttcatcgt cggcgggggc ggctgcaatc tctccgacgt
    57781 ccggccgctc gacggctcga cgacgccgga gtcgaaggtc acgatcgtgg gccgatgcgg
    57841 cgccgcgctc cgggtcctcg tgcgcgtccc gcaagcggga ggcggttccc cgcaggagtt
    57901 cgtcgcggcc gtcgcggacg gcacgttcgc ggcgggcgac gtcccccttc ccgtcatcgg
    57961 cgagaacgcc ctcgagctcg tctgcgaagg cgcggcgggt gcgccgtcga ccgtcgccca
    58021 ccggatcacg cgactctcgg gcgacggtcc cgtcgtctcg atcgcgtctc ccgcgccggg
    58081 cgcggtcgtt acttcgtcga gcgccacggt gagcggcacg gtctccgacg cgacggccga
    58141 cctctgggtc ggcgggacga aggtcggtgc ggccgcgcgc accgggacct ccttctccca
    58201 gcccgccgtc gccctcaccg agggcccgaa cgtcctcctc gcgagggccg tcgacgcagc
    58261 gggccgatcc ggtgaggcgc gcgtcgtcct ccagcgcgac tctcaggccc cccggctcac
    58321 ggtcgtctgg ccgacgtcgg gcgcccgctt cggcaggcgc ggagatgccc cggtcgtcgc
    58381 ggacgtgacc ggcgtcgtcg acctcggctc cgagccgaac ctccagtccg tcgtcgtttc
    58441 cagcccggca gactcgatca cggcgaccgt cgacccggtc accggcgcct tccaggcgcc
    58501 cggtctcccg cttggagacg cttcgggcag cgttcgcgtg acggtcgtcg cgacggactc
    58561 cgtgggtctc acgacgaccg tgacggtcga cgtcgacgtc gacgccggaa ccgtcgccgc
    58621 ggggccggct ctccgtctcg acgctccggt cgatctcacg cggctcacgg ccgcgtcgtc
    58681 tcccgcgatc gccgtctccg gagaagcctg ggcggcggag ggcgcgacgg tctccgtcaa
    58741 cggcgtgtcg ctcgacccgt ccgcgctcga gtgggagccg gccgcggccg acggtcggcg
    58801 tcacgtcacc ttccagacga cgatcgcggc gccgacgacg gacggtccct tcggagtcat
    58861 cgtccgcgtg gaagaccttc agaagcggtc ggcctcgacg cggcggcttc tcgtgcgcga
    58921 cacggtcgcc ccgggagtgt ccgaggtcgt cccggcgtcg ggcgccacgg gcgtcgactc
    58981 gaacggtctc gtcctggttc tcttctcgga ggaagtctcg agggcatcgc tcacggcgac
    59041 gaacggtttc gtcgtgacgc gcgagggcca gccggagccg atcgtcggca cgttctcggt
    59101 ggccgggagc gccgtcgcgt tcgttccagg tgccgctctc acgcccgggg cgacgtatcg
    59161 ggtcgtcctc ggggtcggcc tttcggacct cgccgggaac ccgctcgagg tcccccacga
    59221 gtcgtccttc acggtggctc cgactctctc cggaacgctg cccgttctcg acgcgccctt
    59281 gccggccgtc gtctgtgcga ccgcgctcga gctgaagggg agcgccccgg ccggagcgag
    59341 ccttcgggtc gtcgtcggcg ccgtcacggt gaatgcgacc gccgatgcga gcgggcgctt
    59401 cgtcctttca gtcccgctcg tcgcgaacgg ttaccacgac gtctccctcc ggatcgtcgg
    59461 ccgcgacggc tcgctcggcc ccgcgctcta cggcctcgtc cggaaggact gctcggcacc
    59521 tttcgtcgag cgtgcggacc tcgaccggga agcgggacgt gtcgtcatcc gcttctcgga
    59581 gcggatggcc cccgccaccg tcacgctctc ggcgacggcc ggagacggcg cgagcgtcgt
    59641 cctttccctc gaggaagaac cgaccgtccc gcgtcccggg actctcgtgc tcgacgccga
    59701 cggccggtcg gtgcgcatcg accttccctc cgatgccgac gcctggtggc gcgagaaggc
    59761 ggtccgactg cgtgtccagg agcccgccgc cgacgagaac ggcatcccgc tctcggcccc
    59821 ctgggtcacg acgttcctgc cgggcggcgg gacgggagac ctgaccggcg gcttcctgtc
    59881 gggggaggtc tacgacgacg cgacgggacg cccgctcgac ggagcggacg tacggctcta
    59941 cccgagctcg gcggcgatcc cgggagccgt cgcggcgccg gacgtggcgc tggaggcgac
    60001 ggcgacggat gcacgcggcc gctacggctt cttcggcgac gtcgcggcag ggcggtacgc
    60061 gatccacctc ggaaaggcgg gctacgtccc ggctctccgc aggcttccgc tcgcgcccgc
    60121 gacgggagcg gtccccttcg acgcgcgcct cactccgttg tcccccctcg cggctcagcg
    60181 actcgacccc gtcgtcggcg gcagcttcga gggtccctcc ggtctcctcc tcgaggtcgc
    60241 cgtcccggcg ttcgtcgacg ttccgcagga gacgctcgga gtccggctga ccccgctcac
    60301 ggcgcaggcc ctccccgagc tccttcccct cggctggtcc cctctcgccg cggcggacgt
    60361 gacgctcgag ctcgaaggga cgacgccggt tccgctttcg cagtcggccg ccttcgcggc
    60421 gggcgccgtt catctcaccc tgccgcttcc cgcgaacgtc cctcccgcgg cggcgctcgt
    60481 cgccgtccgg cacgacgtcg cttcaggccg ctggctcacg ctcggtcccg tcgaccgcca
    60541 cgcgggcgcg ggcagcaccg acgtcgcgcg cgttcctctc gtcgcccccg gcgcagtcgc
    60601 ggttgtcctc gccgacgtgg agcccgcgat cgcacctccg gcgctttccg gcgccgaggg
    60661 ggagccgctg accgcgtcgg ccctcccgtc tcccgttccg ccgctcacag ccacgctcac
    60721 cctcgacccg gccgtcgtct cgccaacgga gcgggcgacg gctcgcgtcg tcgcgcgctc
    60781 cgccgatggc acgaccttct ggccttcggg gctcgccgtt caggcctacc tcgacgagaa
    60841 gctcgttctc tccggaggag gagagctcta cgaggctccg ttcacggcgg atctcctcct
    60901 gacgcaccac ccgctctcgc ccgaggagga gagcggtgcc acggcgggca ctgtcggcgc
    60961 tctctctttc gccgtctcgc cgagtccgaa ggccgcggag gtcctcctcg aatccggctg
    61021 ggagaacgtc cgcctctacc cgttcccgga gtcgctcgag cgtggctcga tcctcggcgc
    61081 cctcggcggc accgtcacct cgcccgacgg cgtcgagctg acgctccccg agggcgcgct
    61141 cgccgagagc gtcgccgtcg aggcgaagct cctcaccgct gccgagcttt ccgcgctccc
    61201 ggcgcccgcg ggcttcgacc tcctcgccgc cgtgcgcgtc tctctctccg ggcgggcgct
    61261 cgcgcgagcc gcgacgcttt ccgttccgct ccccgcggac tcacccgacg accccgccgc
    61321 ggaggcgcgc ctcctcctcg cgcagctcgt ggaacagccg gccgacggcc gaggcgcctt
    61381 cgcccgcctg acggcgcgcg ccacgcgcgc cgcgggcccg cgcgccctcg ccgccccgga
    61441 ggccgcgggc tcgcctctgc cgctcgaagg gctcctctcc gaggggacct acctcgtcct
    61501 ccgtgcgcag gccccgctcg gcttcgcgac cggcttcgtg atggtcggcc agggcaacgg
    61561 tctcgcgacc tcgcgcgtga cgacgccgac ccttggcacg gcagacctct cgcgccccgg
    61621 cggccgctac gcgatcccgg tcccggccgg cgacaaccgc gacgtcttcg ccctccaccc
    61681 gcagctcgac gaagccgcga cgggccgcat cccgtccctc gcgccgtcgg ccgtcgtcgc
    61741 tctcgacctc ctcgtcaagc ccgtcggacc gcacgtcacc tcggtccagc ccggcgacaa
    61801 cgcgacctcc caggccatcg gctcgtccct cgtcgtccat ttcagcgagc cgatcgaccc
    61861 cgcgtcggtc gtccccggtc tcctcaccgc cgagctcctc ggcgacgacg gcgctgcgac
    61921 aggcgccttc ttcaacggga ctctcactct ccagcccgac ggcacgaccc tcgtcttcca
    61981 gccgacgcat cccctcccgc ccgggcggcg catccgcggt cgtctcacct ccggcgtccg
    62041 cgatgtcggc ggcaccccct acgaaggggc gctcccgtac ctctggagct tcacgacctc
    62101 gacggaggtg acctccggcg ggcagatcga cctcacgaag attcgccttc tcctccccga
    62161 gaacggcacg gcccgcatcg tcggcaccgc gggcgcgatc ccgaccggtt ggtcggtctc
    62221 cgcgagcgtc gagaacgcca aaccctcgcc cgcctgcccc gagacgacga cgaacgtcga
    62281 cacgaccggc gccttctcca tcgttgcggg ctgcgcctcc acgcccgtct ccctctcttc
    62341 gcgcgtcttc ctccgggccc tcgactctgc gggcaacctc accgttctcc cgctcggccc
    62401 ctacgtcacc gcggacggcc tcggcttcgt cgcgcaaccc ggcgagaagg ccgtctacac
    62461 gaccccggag ggagtcgaag tcacggtacc cgccggtgcg ttcgacgagg cgaaggtggt
    62521 gcgggtcgcg aagaaggacc cggcgacgct ggggttcgat ctcccgacct acctcgagct
    62581 cggcgcgttc gtcgacgtcg acttcgacgg cgaggcgagc gagacgcttc gcctccggat
    62641 tcccgtcacg acgacggcgc ccgtcgggac gctcgtcttc gcgggctccc cgattgatct
    62701 cccctggggt cggaagctcc ggcttatcga cctggcccgg atcgtcgacg gcggcggggg
    62761 agcgaaggtc atctcgaacg cggaggccga ccagcccgtc gacccaacga cgggcggctc
    62821 agggctgatc gctctcgagg gggagacgca tctcgcgagc cagctccctg cctcggtcat
    62881 ccgaatgacg ctcctcgaat tcgcgagccg gggctcggcc gcgttctgct tcggagtggg
    62941 cgctgaactg actgcagtca cgggctcaac ggttccgaac gagctgatga cgcaggtcgg
    63001 agtgctctgg aacgagatgg ccgacgcctt ggtcttcgtt cctccggcct acaactggac
    63061 gggtcgcttc attctccccg cagtcgtagg gcaggcattc acgatcgtcg cgaaagaccg
    63121 ggcgaccggc tgggtcatca acaggcacga gttcggtcca gtctccccgg ttcccggagc
    63181 gctcgttccc gtcacggatt tccccgacaa cgacgtgacg atcccgaggc tgatcgatgc
    63241 gagcccgttt ggcctcacgc ggttctcggc tttcaccgcc gatggtacag agccgacatg
    63301 cctctccatt cgcctcgagc ttcgcgcctg cgccacgtcc gacggttttc tccgaatcga
    63361 ggccgatccg gcctacccgc tccctgacga gtctcgggtc tccctgcagt ccctggcgtc
    63421 gcctgccggg gacctctccg cgcgtcgcgt cctgaacggc tcgctcggtt ccgagccgct
    63481 ccagctcgcg ccgaagagcg gcgaggatct cctcgtgacg gtgacacccg gggacctgaa
    63541 tccggatggc ctcgaggtgc tcaggttcgt tttcgacaag cctcttgccc ccgtgccggc
    63601 ccccgagacc gcggcccatc tcgtcgactg cggcccgttg ccaggcgatt gccggtccgc
    63661 ggtcccgttc cccgtcgaac tggacgcccc cggcggtatc catgcgccat tctcggaggc
    63721 gctcgtccgg ctccgaggga cgctcccgcg cgggcacctc ttccggctga cgcttcagcg
    63781 cgatcagttc cggagggcga cgtccggatc agacgcagtc tactccggcc cgtcagattt
    63841 cctcttcgcg acgaggaaag ccgctgaaga gcccctcgcg acgagtgacg ccctcgccct
    63901 cggcgacacg aatctggcgc gcgacctcgt gaagctcggc aacctccttt tcgtcgcgac
    63961 cggcacgggc cggctcctcg cgttcgacgt ctccaccgcg cggagcgggg atgttgcggg
    64021 aggagcccct tctccgttcg agctcttcgc gcggatgacc gattcgttcg acgagattcg
    64081 gggcctcgcc acggacgggc acaaccgcat cttcctcaac gtccgggccg gcaatacctg
    64141 gggcgtcaag gtcgtgaggc tcgaggacgt gcggggggcc ggagactgcc cggacggcga
    64201 ttcaccctcc tgggcggagg gcgtcccatg cttcagcccg gtcgaaggga gtgtccgaac
    64261 ggccttcctg gcggaggggg cgatgctcgg ctccgagtat ctcgcccgtg tcggcagtct
    64321 ggccgtcggg cggccggtcg ggctcgagct gctcgcaaag gacgacggaa cgaaacctac
    64381 ggatctcaag agcttctacg aggagaacaa gccgcccggc gacccggcgt tcgacgagct
    64441 tccccgcgac gcgcagggct tcgtgacctt cgggttgctg ctctcaacgg tcgaccacca
    64501 cgccgcgcgg tcgacgagga cctgctccgg agaggccatt gatgacagat accagcgtgt
    64561 caccgtcgac aacgtcacga cggggcagag ctggtccttc gatgtcgaaa acgcctggcc
    64621 cggaacgtct ccgccgagcc ccggggtcag gatcgtctcc ggtctgaagg cacgtcgagg
    64681 tgacgagttc cggctgcgcg tcaacgtcgg gctcacgggt tacgtcccgg tcctcggcag
    64741 cggcgtgacc gtcgtcgatc tgaatcgttt ctaccggctc ccggcatccg gaagcccgga
    64801 cttcgagggc cagtgcggac gccggctcgg ccggtacgag gaggcaggct ccttcggcgt
    64861 cgcgccctgc tccgccggga agacccccga cctttccaac acctacgcgg ccgccgtgct
    64921 cggccagacc ggagacgagg ctgagccccg aggtcgcgac acccgtagca ccttcctcgt
    64981 cgtcaaccac tacggcggcg tcgaggcgga atcctccacg agtgacctcg gagatcttca
    65041 gaccgttcag ggcgtctgtc tcacggattt cgacaccgat ccgtctctac ccgtctctct
    65101 ccgaagcgtt gccgtagcca cgaacgttcg gtggcgggac gaagggctcg tcttcgcggg
    65161 gggccgcttc gtcgcgaaag gcctggggat cgaatcggcc gaacgtggcg gagatctcgt
    65221 cttctactct ctcggccccg aaggaatcgt cgccttcgac gccacgaacc gtcgtctcgg
    65281 cgcgccgatt ggcctctact accgcaagga ccactgggtc acacgtatcc aggccgacgc
    65341 gaggggtggc cggctctacg cgggcggatt ccacgcgatt acgaacgagc cgttcatcga
    65401 cgtctgggac ctctaccggg tcaacggtgg cccggacgag gagggccggg atgcgaggct
    65461 tctcctcagt ctgaaggcgg catgggacac gaaccacatc gcgttcgatg aagccgggac
    65521 ggggttcctg tacacatggg ggagcgcggt cgccggcgag gccgcacgcg gtttcatact
    65581 gccgatcgaa gatgcgcggt tcgtcttcgc agggttgtat agccctgaag cggcggagcc
    65641 gccgagtgac ccgccggccg tgggtcctct cccgacgctc cggcccacat cggctttcgt
    65701 acccctcggc gtgccccttc gggtcgactc cgtcgccgag tgtgaccccg ccaatcaagc
    65761 cgaagacgag aaggtcgcca ccgcggcctt ccgcgttcgc attgctctgc ctggggacct
    65821 cggggagacg ctcgtcgcga aggtgcagtc cctgcgcggg cttcctgcgg aggggcagct
    65881 cggcgccgag gatgtcggcg ctttcgtggc gccgacgggt gggccgggat ggccagcgag
    65941 ggaggtcttc atcacccttc gccggctggg caccgccgac gcctccgcag ggaacggcgc
    66001 ctatcccggc gaaggcggtc gcctctcgac ggcctacaac ctctacgagt cgaaggaggt
    66061 cgtcctcctc cttgctgacc cgcgagcccg cgcgacctac acgctccaga agctcgaagg
    66121 cgaggccacc gccgacgagg agagccagtg ccgccgctgc acgaggccgg cctacctcca
    66181 gcgcctcatc gacgacggtc tcctccaggc cgacgacatc ctcgagctcc tggcggcggg
    66241 tccatacgtc cgtgcgacgc tttcgacgga acggaccgac ccgctcgaca cgagcgtcgc
    66301 cgtgcccgcg gactctggcg ccaccgccca ggcccgcgtc ttcttccaag gcaacagcgc
    66361 cggatatcgg gcgccgtggg gcctggcacg cgcgacatcg tgggccgatt cggttcccgc
    66421 accgatccag gtctcgctgg aggagcccgt cctctcgccg gcctactggg attcgggcga
    66481 gaggggagtc ggcgtcctcc tcggaggtgg cgaggccgtc ttcaccgcga ccgactacgc
    66541 aaagccgggt cggggaatcg atgtcgcgat ggagcgcacg taccgctccg gcgttctcgg
    66601 tttcggccct ctcgggtcgg ccggctgggg ctcgccgctg ctccagcacc tgcggcagat
    66661 cccgctctac ggcgacggga agcggccgtc gggcgagggg ccgtcgtcgc tcgaccagct
    66721 cacggggatt cccgagatgg tcgagtacta cgacggtacc gggcacgtct tccgattcat
    66781 ctcgaagggg aacggtggtt gcccgacctg ggccgaggag gactccctcg ggagctactg
    66841 cgtgcccaag ggcctctacc tcaggctccg aaagctcgag gggggcaagc ggtggcagct
    66901 cctggggcgg cagcagagcg tccttctctt cgacgaacac ggtcgactcc tggaggttcg
    66961 cgaccgtcac cgcgagctcg ccaccgatcc tcatgtccga ggcaacacgc atcagctcgt
    67021 ttacgacgcc ttcggcgcct tggcgcagct cgaggacgac tacggccgca tcctccgatt
    67081 cgaggagttc tcgaatcccc gagaggacgg agagcagtac ggcctcttga agagcttcac
    67141 ggatttcgcg gggcgaaaga cccgctacat ctggagaagc cagccgggga cacgcgacga
    67201 caagctcctt cggaaggcca ttctcccttc tgtgacgagt tcactcggag cggattactc
    67261 tcatgcggac ccgaccgtct cctattccta tggcgagacg atcctcgccc cgtcggcacc
    67321 gcatcacggg cccgacttca gccggctccg tctgaccggc ttcaggctgc cgggaacaga
    67381 cgagagcgcg ccgagcaggg ttggcctcga ctatgacgcg tcgtcggggc gggtcgtagg
    67441 cgtgaccgtt cccgacggcg gccgttggga gatccagaag gccgagggcc cgcccgcggc
    67501 cccggcgact tccgcccgtg tcgttgcgcc ctgggggcac gcgctcgact acacgctgga
    67561 atcggggcgc accgcgacca cgctcgaagg tgtgcctgcg ctcctggccg ggatccccgt
    67621 caccggcccc gacgcttctc cagctgacca ctacgccctc accatcttcg gctacgagga
    67681 ggacggacgt ctcgggaagg tcacgatccc gggaggactc gagcgatccc tgtcctacga
    67741 gagctccgcc ggcgaccgac ttcggaacgc gaacgtcgtg acggtggtcg agaaggggtt
    67801 cgctcgccag ggggacgcgg gatgctcgat cggggccctg tccgaagtca cccggacgac
    67861 gacgctggag tacgatcccg ccaacgctcc acacaaggtg aagacgcctc gcgatgtcct
    67921 cctgaccctc gcgatcgcaa catcggcgtc gggagcggtg cccgtttcag tgccgttcgg
    67981 catttcggcg gcaactggta ttagggagct cgaggcctct agcagcttcg actcgcacgc
    68041 acgcaatgtg aggaccgccg gccccgaagg ggagcagggc acccccgtcg tcgaggtcac
    68101 ggacttccac gacgatgctc ggggacggcc gcaaaaaggc tacctcaaga ccgtcgtccg
    68161 tggcccgata gtcgagtcct acgagcgcta cgacgccgca ggtaacccgc agacccgcgc
    68221 tttcggcggc gtagtagaaa cgagtcgatt cgacgagtgg gaccggccgg tgcagatcga
    68281 agccgggggc tcggacggga cgtactcgcc gacgaatgcc cggatccagc gcggcttcga
    68341 cgcggctgga cgtctcgtcc tggagaggcg gagacagacg ccgctcggcg acgtcgacac
    68401 catcactgcc tacgacagcg ccgaccgagt cacctctgtc acgatcaacc gcgtcgccga
    68461 tgcttccgga gacgccaccg cgacggccac gactcgtttc gactacgacg cgtatggccg
    68521 gctctggaag gtcacctctc ctgccggggt cgtcgccatc tacgactacg atacggcggg
    68581 ccggatcagc tccgagacga ccggctcggg tgcacggcgg cggctctacg acgaacgggg
    68641 ccgtctcgtc tacaccaccg atgggcacga ggggtactgg cggggtctct acgacgactt
    68701 cggcgggctc accgaggaac ggatcgccac cggggccgtc acgcgtcgtt gcttcaacgc
    68761 ggaaggccgg cccactcggg aggaggtccg ccaggccgac ggaaaggtcc tctccgacgt
    68821 cagccgggaa tacacctcct tcggggccgt ctccaccctc gtcgagcgcc tctcttccgg
    68881 cagccccacg ccggagcagc gcgtgacccg gatgtcctac gaccgctccg gccggctcct
    68941 gaccgtcaag acgggggatg gggatacgct cgttcgggac gaggcgacgc tggtctacga
    69001 cagcgccaac cgcaccgtac agcgtaccga tgcggcggga aacaaggtcg acaccgtctt
    69061 cgagcccgcc tcgcgactct tcccctcgag cgtcaggctg accgagtccg ggcgttcacc
    69121 ctattccacc gacttcctcc tcaccgacgc tgccggcaat gtccttttcc agcgcggaag
    69181 cgacggatcc acggtcctgc gtgccttcga cgaggcgggc cgtgtcacct ccgaagtcat
    69241 gggggcaaac cctccagcca cctacagctg ggacggtgcg ggccgtctgc tcgcgagcac
    69301 cctcccggcg ggggggcaga cgacctaccg ctacgacctc gacggccgcg tcacttccga
    69361 gactgtgacg agctccacag gcgcatggat cacctcccac ggctacgacg tcgcgcactc
    69421 cggccgcctg acgggcgtca cctttcccga cggcaccgcg gaggtctaca cccagttcga
    69481 tcctgacgat gtcccgcgcc aatggaccaa ccggcacggc caggtggtca gcagcgtcgt
    69541 cgaccctgcc aatcgcctcc ggggcctcac cccctctggc ggtcccgcga acctggccac
    69601 gtacagcctc tcgtgggact acgatgacct ctcgcgactc actcgagcag agaagacggg
    69661 agtccccgcc tcggccgtcg tcaacagcta cgacctcggg gggcgtccgg aaacgaccac
    69721 gctcggtatc ggtgcgacct tcacccgcag ccacgatgtc tggggccagc cgttccggag
    69781 cgcaatcaca tcggagatgt cgggcgacgc ggccgcgggc gtcgccgtcc tgcggcgcga
    69841 tcacgacgct ctgagccgcc ccacacgcag ctccctcgat caggacgggg gacccgatgg
    69901 gagcggcatc cctggcgcca gactttcctg gagcggcgcc agcaacctcg agctcatcgc
    69961 caccctcggc ccgagaagct tccagcagac ccgttcgtac gacagcccgg gtcgcactac
    70021 ctccttcggg ttcgtcggaa gccgggcctg gggtgatgtc ggagtcgcct accgcccgga
    70081 tgattcctac aagacgggac gtgtcgcacc ctccgccggc ctcttcagcg gccagggctg
    70141 gagcttcacc cccgacgtac gccttcgcct gacgtcggcg gagagttccc gagagacctt
    70201 ccatctctcc tacggggccg gagacgagct cctcggccac acccgagaca agcagtcgct
    70261 cctcgcgagc ttcagcccgg gtcctggcgg acgcctcctc tcccgcaacg gtgacgcgtt
    70321 cgtctacaac gcctcgggag accggacgga ggacgaccgg ttcgtgtacg ggtggagttg
    70381 gcgaggtgag ctggtctcgc tggaggttaa gggcgtatgg ccggcggatg gtcccggtga
    70441 ggagccccgc gtctccccgt gggccgggca caagctcttc ttcgactacg acgccctggg
    70501 tcgcctcacg gcgcgcacgc acctcggcgt cctgccgagt cccgtggacc ccgatacctc
    70561 gaggcccttc atctcccgcc gggaggtcct ttgggacggc ggaaccctcc tcgcggaagt
    70621 cgagaaggac gcgcaagggg cgatccgctg gaggaagacg ctcgtccccg gcgcgaccgg
    70681 gctcgacgac gcggtccaga tgcgcgtgga ggattacgga gccggcaaca ctcccgcccg
    70741 gcgcctctac agcttcgtga gggacgagca ggggaccgtc cttggcctcg tcgacgaaca
    70801 ggcgccggaa ccggctcgag gacccccgct tccggcacgg tatctctaca cgccgctcgg
    70861 cgaagcgcac gccgagacgg gacctgagct cctcagggct cgcttcgacg cgaccaggac
    70921 ttccctgaac ggcgcttccc agaaccctcc ccgtcccgac gagaccgtcg gcggtgccgt
    70981 cctcttcgac ctcagcctca cgctcgaccc ggagacgctg gggcagggag tcccgatcga
    71041 gacctggagc tccgccgaca acgcctggca gcgggtccct gcggggaggt tcttgctcgc
    71101 gttggaccag gacaggccga cgagtatctc ggttctgccg ctcgatggct ggccgaagga
    71161 gacccgctac cgaatccggc tgacctcgga cctcctcgac gatctggagc gcccattccg
    71221 agctccggac ggcggagtca gcttcgacgt ccttctcgaa atgccggcag atggcctgac
    71281 acaccctgtc tacgaccgga cgattccgac gaccttcgac acggcccggg ccgcgagcga
    71341 cacggtcgga ggccgagttc cgggcggcat gaacctcctt cacgccggtg cgtggacgga
    71401 tccggtgtcg ggattgcagt acctgcgggc gcgctggtac gacccgagga cggggacatt
    71461 cctctccgaa gaccccgcag gagacgtcga ctcgccgaat cgatacgcct atg
//
LOCUS       DAOONL010000008        70607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_230_length_70607_cov_14.219980, whole genome shotgun sequence.
ACCESSION   DAOONL010000008 DAOONL010000000
VERSION     DAOONL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 70607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70607
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_70607_cov_14.219980"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLP50_02300"
     CDS             <1..468
                     /locus_tag="PLP50_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50410.1"
                     /translation="LGGAATGVLVSPWLLAFSVFFFLSLAFVKRASELVSVRERGLGS
                     AAGRDWRTVDGSTVPVLGAASGYLSAVVLAIYIHSDVTKRLYAHPGWLWLLVPILLYW
                     VSRLWVLVGRGEMDDDPVLFAARDAVTWGLAAATAAALLLAALGPTGIPGLME"
     gene            592..3570
                     /locus_tag="PLP50_02305"
     CDS             592..3570
                     /locus_tag="PLP50_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA50411.1"
                     /translation="MVRIRTAPTVLLCLALLLLSLGAFAQSAATTGAIEGTVADEAGG
                     VLPGARVVLRNTATNYETTVATAANGRFRGILLPLGPYRLTVTLPGFAKVVREGLNVS
                     VGQSVNVAVTMRLAAKAEEVLVTAENPVVETTRPEGTTRIDTLAVRSLPNNGRNFMDF
                     TKLTPGVSIVQGPDGDELSINGQKGIQNNVSVDGADFNNPFFGEQRGGQRPAFTFNLD
                     AVQEVVVVADGANAEYGRSSSGFVNVVTKSGTNQLAGTVHVFYKDDSLASAARRPDGS
                     SADEHDSNQVQGGFTLGGPVATDRIFFFAAVDAQGGSSTKQTDPSRIEQRVVDYFASL
                     GYPDENGSIERGNDAFVALAKVDWLLGSKHTVTLRGTYTKSSQENGTFDVDSWGRSAN
                     ATEDNWSRSLTGTAISNFGSILNEFRFQLAREDRPRPYDGPNIAGQDRPLPDTAFDFG
                     RGYRFGMPFFIPVDYYDTRIQLNDNVTVLTGAHELKAGVELNRVNAAQVFRGFANGRY
                     IFSSTDGFLSYARNPSYVECSDGASSPDGTCPVGTTVTGPVLLFLQQAGVGGLTAEEA
                     GTQAIRQDEPAVFVQDKWQPTSNLTVQLGLRWEAQIQPDPITPAEDVFYAGFIGKTSL
                     GQEFPSDGNIPSDRSMWQPRVGITWDPTNDGRTVVRANAGLYYARIPGLTFASTRSTN
                     GSRGQSLYRDSSLTRILGPVPAYPNLIPQSEVGSPFRPDVYVVARDFQNPRTVAASVG
                     IEREVMKDYGVLVKYNYAKTTHLTRFVNRNDALLGSPWSSGLNGGPNGIGTLTVVESS
                     ARSLYQGITFGVSKRWSNDYQFQLNYTLSWDESDDDNERDPFSFRYAKVTDLDAEYGY
                     SDRDQRHRLNGIFVWRAPGDVNVNLRYSYRSAQPKSITLAGADANTPQDRINPDGTVT
                     QRNLGRKDNQFSSLDVRLSRSFDLGPIQVEPILECFNVFNSKNLKRPEVTNLIFNFDG
                     TVQSGLGDPRQVQLGLRLAF"
     gene            3670..4107
                     /locus_tag="PLP50_02310"
     CDS             3670..4107
                     /locus_tag="PLP50_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPA50412.1"
                     /translation="MTETTAAQPLTRITTTATFSAAHRLHNPSRDDEWNRATFGKCNN
                     PHGHGHTYTVEVTVEGSIDPVTGWVLDFSHLKRIVDEKVVRRCDHRNLNVDVDFLAGV
                     VPTAENLAVRFWNELAPHVAPARLVKLSLHETERNRVVYRGGA"
     gene            4107..4883
                     /gene="surE"
                     /locus_tag="PLP50_02315"
     CDS             4107..4883
                     /gene="surE"
                     /locus_tag="PLP50_02315"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPA50413.1"
                     /translation="MRALVSNDDGIHSPGIEALERAARAAGFETWVVAPDREQSATSH
                     ALTLTRPLRVVKTAERSWVVDGTPTDCVNLAICRILKDVRPDFVFSGINLGPNLGDDV
                     TYSGTVAAAFEGTLLGVPSIAFSLDCRKDGGDGPDFSEAERIAEEVIRLALSRPMAED
                     VLWNVNIPEGRPTGIRATRMGRRRYGESVVEKVDPRGRPYYWIGGSHVDTHSDGSDLT
                     TVAEGLVSVTPLHLDLTAYRALEDLSARLAGSRANGADRS"
     gene            4893..5594
                     /locus_tag="PLP50_02320"
     CDS             4893..5594
                     /locus_tag="PLP50_02320"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPA50414.1"
                     /translation="MRPEDPEVARAARAGAEARRAMVARIVAAGLLSDRQVVEALASV
                     PRHLFVPRELAGEAYGDHALPIGAGQTITQAANVGRAAELAAIPSGGRVLEVGTGSGY
                     QAAVLARLAGQVFTLERIPQLAAEARRVLALLGIGNVSVKVFDGTYGWREHAPYDAIV
                     VAAGAPEVPAPLVSQLSATGRLVVPVGPPGRQRLLVVKKLPSGRTRTEDAGEVAYVPL
                     VGKFGWEKAPAESRR"
     gene            5591..5893
                     /locus_tag="PLP50_02325"
     CDS             5591..5893
                     /locus_tag="PLP50_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPA50415.1"
                     /translation="MSGGFENAARRWLVAGRVQGVGFRAFAARTARGLGLVGGASNLP
                     DGRVLVVAAGAAHALDRLESALWEGPRFARVQSVEAAPAAPDEVLASFDAEFRLDR"
     gene            5908..>7169
                     /locus_tag="PLP50_02330"
     CDS             5908..>7169
                     /locus_tag="PLP50_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50416.1"
                     /translation="MSKRRILVVDDDADLRALVEGQLRAAGYEPTGAANGSEGVERAV
                     SDRPDLVLLDVGLPGLSGWEVCATLRSLAETSSIPIVMLTGRSEIRDFVTGRQAGATD
                     FVTKPFARAKLLETVENALAGKDGSSPVPYGPELPERRARGLLLDALTGLPAISVVVD
                     SLRERLLGDEEFGVLLVDVDAAGALEEHYGWEVLDDVVREAGRSLRRLVGTLFSTRDL
                     LAVRRPGSTAMVVFVGLPAGLGEGEAATRLDRKARQVAESLGALLGERFLGRVHRRLQ
                     VTAAASRLRYSPQVRLERLVERALAEASAVASTREGERAAAQKAEFDALLRVGRLEVV
                     FQPIRDLASMKVIAREALTRGPEGSSFESPEVLFEYAARHGRVLSLEQACLALSATRF
                     VPVAEELLFVNVETHVVSELAHGGLEVL"
     assembly_gap    7170..7269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7270..7724
                     /locus_tag="PLP50_02335"
     CDS             <7270..7724
                     /locus_tag="PLP50_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA50417.1"
                     /translation="VETHVVSELAHGGLEVLNPLVALGPSVVLEITERGAIADFEVFR
                     RGLSALRRAGFRIALDDAGSGHASLHALAELRPDYLKISQFLVSGLHRDGIKSEIVAM
                     LVRLGERIGAVTVAEGIETDEELAAVRELGVALGQGFLLGRPEGATRR"
     gene            7782..8195
                     /locus_tag="PLP50_02340"
     CDS             7782..8195
                     /locus_tag="PLP50_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50418.1"
                     /translation="MTEHEDGGPELPDGFEKVEATVCRRGGELLVFGTLEPEGPFVFR
                     LQLAKKVEDFGPPPPGFEWTGEERPPKKGEWFWSELRGRAVQAIKDETGVNQFGEPTG
                     GRRILRPAGTAGRPTPTPKHGLRLVRNDEGPEGSG"
     gene            complement(8187..8588)
                     /locus_tag="PLP50_02345"
     CDS             complement(8187..8588)
                     /locus_tag="PLP50_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50419.1"
                     /translation="MHAGSPHLAVFRLAEVAEVETLVDGEPADIRIEVFEDAANPGQF
                     RCRTWRLLEVGVHPSGPVDADGGLDTEWETLFVPWWVPGGFVTDEPFFAASKESALEA
                     VLDDLRVYLAVTRHAEDGGEPPAGSPPPASA"
     gene            complement(8624..8700)
                     /locus_tag="PLP50_02350"
     tRNA            complement(8624..8700)
                     /locus_tag="PLP50_02350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8664..8666),aa:Arg,seq:cct)
     gene            complement(8747..8977)
                     /locus_tag="PLP50_02355"
     CDS             complement(8747..8977)
                     /locus_tag="PLP50_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50420.1"
                     /translation="MDRKHLHTFQLVVEGLADPREFLMPFESDDVILATDACARYIAR
                     QEDNFLPLGADQAVRANRVLLVKHVSVGPFEE"
     gene            9043..9756
                     /locus_tag="PLP50_02360"
     CDS             9043..9756
                     /locus_tag="PLP50_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPA50421.1"
                     /translation="MSEITDRIEVSRETPAYPARHDVRDGHLVVGGERVPVVVKRIPR
                     DFRQPDGRTRAERSLRIARHLLAHGLDTPEPLGIEVTPEESVLVVRKVERAVEVRAFF
                     LRRGDPSLGTLPLRSSFDEVLVALARFARRMHDSGVFFRDFSDGNVLVTDGEEGPRLW
                     IVDLDRARIRRPPLATFTRLRDLSRPGLTRADDRRRLLEAYWAPGPVPAGAAALHAAL
                     RFRLLAWNRLKAVLRPWRR"
     gene            9881..10504
                     /locus_tag="PLP50_02365"
     CDS             9881..10504
                     /locus_tag="PLP50_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50422.1"
                     /translation="MANDLPGEGAPAPEPGIREREKELLDRVRGGETDAFEHFVKTYQ
                     RKVFRLVYTLVRNAADADALTQDVFVKAWRAVADFKGEAAFETWLNRIAVNAVRDAAR
                     RRRPVVSFAELADAEGTGGAELPPSMEPVDATTPEREARSRQIRRRIREALDALSPRQ
                     KAIFVMKHYEDRTVAEIGAAIGVDDGTVKSHLFRAARKLRQKLEDLR"
     gene            10501..11037
                     /locus_tag="PLP50_02370"
     CDS             10501..11037
                     /locus_tag="PLP50_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50423.1"
                     /translation="MSGRHLTEDEVVNRVFPAQDGTAPIPIHQAVCPECQERVARLRE
                     AFLLDRGALTGVVESLPPEFWTAQRKAVMGRVSELAALEAEEKATPFPTRLTRSVLQR
                     PALAFGSLAAALTLVAGLTVLRGGDDRPAEPEQTVAVAEGTAALSAADSADDELLRAV
                     DVALTTESPYETVLPEAM"
     gene            11037..11597
                     /locus_tag="PLP50_02375"
     CDS             11037..11597
                     /locus_tag="PLP50_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPA50424.1"
                     /translation="MRRTLLLPALLGAVLAASPAPAQMLEMPPGKWWKRPAVVEALRL
                     TPEQQQRLEEVFSKNRRTFVDLKADVDRRTIDLEDLLAAREVEPRRVAAASEALEQAR
                     ARLGKARTMMVVEMRGVLTGDQWATIVDRREEFRAERRAEVRRRFADRRNGGRPGRPM
                     ARPGTPAPSAPPDPDEGPDVPDEPEP"
     gene            complement(11594..12376)
                     /locus_tag="PLP50_02380"
     CDS             complement(11594..12376)
                     /locus_tag="PLP50_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA50425.1"
                     /translation="MTTARRPPYAVLAGYYDEVYGLWRDLLGPARDEILRAHRVRFET
                     LLDVGCGSGWQSLELARRGARVVAVDPAVPFLRPLRAAARRERLRIDVRRGDLLSLPL
                     GVGETFDLALATFDVLNHLDRHEELGPALAGIAKALAPGGHVLFDLNSPETIEAFPDH
                     HRVTHLSGGGLSAERGSFDPESGAGTVTRDWFLPVEGAPGRFRRVRELYREIAWREAA
                     VRRALRVAGLGVRLFADASGWLSFQPEGTRWIVLARRRPGTR"
     gene            complement(12373..13233)
                     /locus_tag="PLP50_02385"
     CDS             complement(12373..13233)
                     /locus_tag="PLP50_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50426.1"
                     /translation="MTGFIEIALLLAAPLLLAATGELLLERAGSIQIGLEGTMLFGAW
                     VAFAAGRAGLPAWGGLLSAALAGLGAGLLFALFAVVRRADPILVGTVWNLLALGATAF
                     AYRLGAGETGSVLEVATLPRGLVGLPDAVAASFLAPVVLHLWLTRTRAGLRLSAAGEN
                     PEALRSLGLSVVAVRSGAAAASGVLGAMGGAVLVLTVSPTFVEGVTAGRGFLALALVV
                     FARWRPLALLPAALLLGGVTALQYRLQAEGFGLPYALFLALPGLVALGALALASARGG
                     APKALGSPAP"
     gene            complement(13230..14225)
                     /locus_tag="PLP50_02390"
     CDS             complement(13230..14225)
                     /locus_tag="PLP50_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50427.1"
                     /translation="MRPAAVAAVAAGTLAAAALLVLAAGASPADAFVALLRGALGSSA
                     ALGETLARSTGLVLTALGAVVAFRAGVLNIGLEGQFLAGAAAAAAAGPLFPDAPLAAR
                     AAVLLAAPLAGALQAAPAAWLAERRQVPAVLSTILLNLVAAAGVTWLVRGPLRDPSGD
                     YPQSRALAEAALLPPLAAGSRATAAVLLALLLAAAAAFVLARTPIGLALRAVGEAPRA
                     ARAAGLPDVRLRVGAFLSSGALAGLAGGLEVLALTGRLYDPFTAGVGYTGIAAALLGG
                     MSTIGGAASALLFAALGAGSSALQRDAGVPSSLASIVPALAVLALLLARGERRER"
     gene            complement(14222..15721)
                     /locus_tag="PLP50_02395"
     CDS             complement(14222..15721)
                     /locus_tag="PLP50_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA50428.1"
                     /translation="MSDPGAALELNGIRKAFGPVTALDGARLTVRAGELHALLGENGA
                     GKSTLVAIAAGLLRADAGEVRRDGALLPAADARAARRAGIALLPQHDLLVEAATVADN
                     LALLDPAAPPFESAGARRARVARLEERLGLELGPPGARVGELPVGVRQRIAVAGILQA
                     DPGVLVLDEPTAVLSPRETSALFRSLRARVATGRAVLLITHRLAEVFEGADRLTLLSR
                     GRTLLEAEVSSTTPEAVGALLLGGAEVSSRSGALRPDPPDGERVPALAVDELLPAGAT
                     GPPASLAVPAGAALTLLAVDGNGADLVGSAIAGVRPARGRIRLSGRPVPPGDPRAFRA
                     AGGAFIPADRRAEGLVPGLSLAENLFLRRPPGRFVIDRAALAAAADDRLRRFGVRARG
                     PWAKAGELSGGNQQKLLLARELEPLPRLLVAVHPTRGLDLASSSDVRARLLDAARAGA
                     GVLVVTADPEEALELGAPIRVVYRGALSPPLAATTPLAELGRRMAGLAP"
     gene            complement(15753..16745)
                     /locus_tag="PLP50_02400"
     CDS             complement(15753..16745)
                     /locus_tag="PLP50_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HPA50429.1"
                     /translation="MTTRRRLLASLLALPLVASLAACGRTPNATPAEGFRVALLTPGP
                     VSDAGWNAGAFEGLKAIERELGAKVSHVQVKTPSEFEEQFRAYAAGGAALCIGHGFEF
                     QDAAARVAKDYPRTVFLTTSGSRTSRNQAPLVFELEQATYLCGLVAGRMSRTAKAGMV
                     GGVEIPSIKSTFLAFEAGLKAGNPGAKATAVFTGSFEDVAGAKTAALALIDGGSDFLF
                     HNANAAALGVFAAATERGVYAFGANRDQNSVSPSVLASAVIDLPRAFVEAARRVKDGR
                     FDGTPIRYGLASGVVSFVWNPALLGRVPAPVVDEVDRAKEKIVSGAIIVPRGNF"
     gene            complement(16765..17484)
                     /locus_tag="PLP50_02405"
     CDS             complement(16765..17484)
                     /locus_tag="PLP50_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA50430.1"
                     /translation="MRPVALVTGASRRLGRALAEGAARAGHDVAVHYRSDPAAAARTV
                     TEIARLGARAEAFRADLRAAGAPQGLVESVLGRFGRLDVLLHSASPWLEKPVAEVTEE
                     DWHDVFSVGPRAAFFLARAAASALADSGGAVLLVSDVAATKAWPRHVPHAVSKAAVDA
                     LVRNLAVAFGPSVRVNGIAPGIVLPPEELPPEELARLVARTPLGRAVSVDDLVSAALF
                     LSSNRSITGQVLAVDAGRSVV"
     gene            17568..19352
                     /locus_tag="PLP50_02410"
     CDS             17568..19352
                     /locus_tag="PLP50_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA50431.1"
                     /translation="MTTPRTLVDVFRNLGATAKPDLLLHKKDGRWVPISTAEFTERVK
                     AVAAALGTLGVPDGGAVVLLSENRPEWPTLDFACQCYGAVLVPVFPTMVADQVEFIVR
                     DCGATAAFASTPEQAAKLLKAQATCPKLKHVFVFDHAGGGGKPFGALVEEGRKAHAAS
                     PAVFDERAERRQPDDLATIIYTSGTTGEPKGAMLMQSNFVSNMLAGCSLLPFDSTAVT
                     MSFLPLAHVFERLVEYCYYHRGATIAFAESIDKLRDNLGEVNPHIFGAVPRVYEKVYA
                     RVMDKVDAGSPAKQRLFRTAIGVAKEVLRLRTEKKTPGGALALQHFLYERLVFRKIRE
                     ALGTRFRFAISGGAPLAKDLAEFFWAIGVEVYEGYGLTETSPVIAVNCPSAWKLGTVG
                     RVMPGVEVRIAEDGEILTRGPHVMKGYYGRPDATAEAIDPDGWFHTGDIGVLDEEGFL
                     RITDRKKEIIVNSNGKNVAPAPIESALKGDPFVAQPVVIGDRRKFLSCLVVPNFEKLR
                     EWAAKNGLGALSDEELVAHPRVEQIYQGIVDRWNEGKPNEQLIRAFKLLPRELTIEGG
                     ELTPTLKVKRRVVDQKFRSVIDGLYKGD"
     gene            complement(19408..24348)
                     /locus_tag="PLP50_02415"
     CDS             complement(19408..24348)
                     /locus_tag="PLP50_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPA50432.1"
                     /translation="MSTALRSFSRLLLPTFLVLAVWLFNPRPAAAQSWPMFQGNPAHT
                     GYVPVSLEPQWFNVRWTKTLGTVPGGTLPVNPVAAGDGRVYATLQGYSDDAQLFALRT
                     SDGATLWSRAFENRPTVNPPSYVNGRVYFQTGNHSTDSWLWALDGSSGTTIFKSPVAE
                     QWPRHYAPTVSAGRVYVNGGYYGGMYGFNGVNGDQIWFAALPFYDQWTPAVDGARAYA
                     YVGEGAPGLYIKKLLDGTDSTPGFIPDPDFDWNGWSMDLAPVLGAYGVLAIHNGRLIS
                     FNPTAGTIGWQLNEGFTGQPSVAGGRIYAANNGGLSVIDESTHTLVWSWTPPPTGTDQ
                     AITQPIIVTNTHVLVSSHGTVYAIDLTTHEAVWTFAARDGQLAIADGKLFVASWDGTL
                     TAISLTSSAKPVSISPTTGPSLGGTPVTISGSGFQTGAVVRIGGIPAVVTSVASNLIN
                     AITGPHVNGLVDVEVENPDGDVGRIRNGFTYVCGGTAPTASVSGGGTVCVGESATLSA
                     ALTGSGPWDLVWSDGFAQNGIAASPATRDVTPAATTTYTIRTVTDGLCAGTASGSATI
                     TVSPTPDTTLTAPARICLGTTATVSIPATTGALYNWTVTNGSIVSGQGTNVMSFVGTT
                     NPVTVSVIATIGSCSTTASRDLPVSLPPSATVAGGATVCAGEAATVSFDLTGAPPFTV
                     RWSDGAVQSVGAAGPGSRSVTPATSTTYTITSLADANGCAGGVATGSAPVTVNPVPSA
                     AIAAAGSYCAGASGLVASVPDAGAGATYAWTIAGGTIAGSTSGPSVTFTPTGASVTLG
                     VTVALPSGCTASSTHVLSLAPAPSATVSGGGTICAGSPITLQAALSGTPPFTLTWSDG
                     MVQSGIATMLATRSVSPATSTTYTVTAVQDASCSGSASGSAAVTVVEAPRAFVSTPSV
                     LICLGGSATVTAQLSGPGPYTLTWSDGVVETVAGPSSTRTVTPYQTETYSVVSVSNET
                     CTVPGSGSTRVIVFPDASAATITAPAEVCAGSGGHVASVPAAGDGTTWVWTATNGTIE
                     SGQGTRSVVFTAGASGDTTLSVTVRAAGGCEVSGSLRVRTSARPPLPVIQAPASLESG
                     ETGAVAEVAGAEGLACTWTISNGAITDGADTCRITFSAGDPGLVVLGVVTRNAKGCAS
                     APGQVEVPVRGLSSVLTAPIVLDVAGPGGNRYATELTLANPSPSAVKVTLRYTPATSL
                     GARGGGTVSETLEGGRQRIVPDLLAWLRTKGLEIPSDGSSQGGTLRVTFENVAAGWSP
                     SASTRTTVSSGAGRAGLSMTAVDPAVANASKVWLFGLRENAADRSHVALANLGETGSL
                     DLRVTLHSGEGAAVPKQVLPVVRLEAGQWLQLNSVLREAGFTAGYALVERIAGSAPFH
                     AYAVFNDNTTHDGSVVPATPAAGLGGTRLVPVVVETGSFTTELVLANPSVRAVKARLW
                     FIESLANPGGYSLGGVTVDLAAGEQKILPNVVSAMRQLGLVVTPAKRDYAGTLAVTFS
                     AAGEPVEGWAGARTATAGSGPGRYGLFCPAPAPDAAPGEAWIFGLIQNAANRSNLALL
                     NAAANLGPVTLRYSVFDGETGRKVATSEPVTLGAGAWMQLNGVLSKWKVAQGYVKVER
                     IDGTAPFLAYAVVNDGGVPGAGTGDGSYVEMIPIVR"
     gene            24640..25344
                     /locus_tag="PLP50_02420"
     CDS             24640..25344
                     /locus_tag="PLP50_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA50433.1"
                     /translation="MAHSAALPAGSKPRTSSGSGRRSLPAADPEKRKRLLHAAIQTFG
                     RRGFHEAKIADIAAEAGVAEGTVYLYFRNKEDLLGCVFDETMDEVLAQARAIRHAEGT
                     AAEKATKLVGLHFSFIGQDRDLASVFQIELRRSARHVERFSRVKLSEYFRLLDGVLKE
                     GIASGELRKDLEPRVAVRILFGAADEILSEWLLSGDAKPVADAEKLVGTLLKGFLFCE
                     NDAKERRPVRRRRTTP"
     gene            25341..26600
                     /locus_tag="PLP50_02425"
     CDS             25341..26600
                     /locus_tag="PLP50_02425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019613667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPA50434.1"
                     /translation="MSRAAYILDGARTAFVKAGTDFKAVTAVDLCRAAAVEAMARSGV
                     EPGEIDQAIFGNIATPPDAANIGRVIALRAGLPKEKPAHSVSRNCASGIESIVQAARL
                     IQTGEADVVLAGGAESMSLIPFLYREDVKAVLVEAGMAKTAGARIGAFKKMPWKQLLN
                     PVIGIKLGLTDPVCGLNMGETAELLAKEGGLTREMQDAFALRSHQRAQAARERLAEEI
                     VAVPLPPAYDKVATLDNGVREGQTMEALAKLRPFFDRAYGSVTAGNSSQITDGAAAVV
                     VASEEFVAKRKLAPLARVAGWGFAGLEPERMGLGPSRSTPVALKHAGVPFGKIGLLEL
                     NEAFAAQVLANLAAFEKDPSLGAVDPEILNPNGGAIALGHPVGASATRLVLTLLREMK
                     RRKVAYGLATLCIGGGQGATIVLEGVA"
     gene            26600..28765
                     /locus_tag="PLP50_02430"
     CDS             26600..28765
                     /locus_tag="PLP50_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPA50435.1"
                     /translation="MAEGKHFRLEVEPDGLAILTFDSPGEKVNKYSTPVMLELDALLD
                     ALAKRTDVKALLLVSGKPDIFIAGADVNEIAKVTDPATIADGVRKGQAVLGKLANLPF
                     PTVAAVEGACVGGGCETLLAMDWRLASDSRKTQIGLPEIKLGILPGWGGTTRLPRIAG
                     LATALDVILAGKVVDSKRAKRMGLVDELVPAPILLDVAKRFARGKVGTRKRSNAAPDG
                     GPLRAVPAKAMEKLLEGVGRPVVFRKARAAVLKETHGHYPAPLAALAAIEKGFGRPLR
                     EALELEVEAAGKLVGSPEMKSLVGLFFRMEEVKKETGVAAGVTPRAAKRIGVLGAGAM
                     GGGIAQLAADKGWPVRMKDIRHEALALGYAQAAKIWQEKLKKRRMTKVDFASRMSLLS
                     GSLGWEGFETCDVTIEAVLEKLAVKQAVLKEWEAVAAPEAIFASNTSTLPITEIAKAA
                     ARPERVVGMHFFNPVHKMPLVEVIFGERTAPEVTATIFDLAKKMGKTPVVVKDAPGFL
                     VNRILAPYLGEGVRLLLEGVPMEAIDKGMREFGMPVGPIELLDDVGIDVAAKGAETLS
                     AAWPERMPIDPAFGKLVTGSRLGRKTKKGFYRYEGDKRVGPDPAAYADLGVASPGSAP
                     ISAEALEARLVYPMVNEAAFCLAEEIVPSPGKLDLAMIFGIGFPPFRGGLCAHADARG
                     LAAVVDALSRLASEKGPRFKPAPLLADLAKQGKTFFGKE"
     gene            28768..30558
                     /locus_tag="PLP50_02435"
     CDS             28768..30558
                     /locus_tag="PLP50_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA50436.1"
                     /translation="MSTVKTDKTDKTVKAETEAPSFLKALYQGRFDESLVFPYPEIPE
                     DTKELVTAFADAYRDFDAANIDSEKIDADHFFPRDVVKGLGDLGAMGMAIPEEYGGSG
                     FSAAAYCKMMEVIGPLDASAAIVIGAHQSIGIKPLLLFGTEEQKRKWLPDLAVAKMVG
                     AFCLTEPEAGSDAASLKTTAVYDPATDEYVLTGTKQWISNGGFARFFTVFARVPEAGV
                     EAGSHKEIACFAVVANEDGTLSGLSRGVEEKKLGLCGSSTCQIILDGCRVPAFNLIGG
                     KGNGFRVAVETLNTGRTSLGAGAVGGCKQMIKLAVEHATQRKQFKTRIADFEMIREKI
                     SEMTAHTYVLESMVYMTAGLVDKKVDYSLEGACCKVWGTELLWKTINESLQIAGGNGF
                     MNEYPYGRGLRDSRINMIFEGTNEILRLFIALTGMKEAGDVLKDFQGALSHPVSQYGV
                     LVDHAKMWMRGTVTSYKLDKVHPSLAPEADQVGKYAAVLRNACETMVYRNRKNVVNRQ
                     YLLHRIADVVMDLYAQIATLSRVTAAIEKKGEEKAKPEIEIVRWFSFQARHRMVANLK
                     ALDKHKDAEATAISNVVYEAGGYPFDLWGW"
     gene            30647..31597
                     /locus_tag="PLP50_02440"
     CDS             30647..31597
                     /locus_tag="PLP50_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA50437.1"
                     /translation="MNAPRVVTVPVPTPFPVGPVNTHLVLGEPLTIVDTGPLTEDAWD
                     ALRAGLARHGVSVGDVERVLLTHGHQDHFGLAARIEREGRARVLGGRLDREHFRGRRN
                     RRLLLDALTRAGFGGLERLTMVAMTAAVDHFAEPLSAWDELAGGEVLPGDGWSVVVRS
                     VPGHTPGSLTFEVPEAGILFTGDTVLRDITPNAIVDEDPERPGETFRSVSRYFASLDS
                     IEAGKSRLLTGHGGRIVGFAAHRAKLEGRYAFRVGQIERALSGRPKSVADLCRRIFPT
                     VRPMDLFLAYSEVLGFLMYLEDLGRAERVPCRPVERWTGR"
     gene            31680..35219
                     /gene="dnaE"
                     /locus_tag="PLP50_02445"
     CDS             31680..35219
                     /gene="dnaE"
                     /locus_tag="PLP50_02445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPA50438.1"
                     /translation="MSSDFVHLHLHTQYSLLDGANRTKELIEHVQKIGMSAVAVTDHG
                     NMFGAFDVQIEAVKKGIKPILGVEAYISPGSRRDRETVKTVDGEGNSYHLTLLAESPE
                     GYRNLSYLVSEAFLSGFYYKPRMDWELLEKHHAGLIALSGCVKSEVSQRLLAGDFAGA
                     KETALRYEALFGKDHYYIELMDHGLPIQRQLMPELLRLSRETGIPVVATNDAHYLRRV
                     DSEAQDVLLCIGMGKTVNDSRRMKFYNSEFYVKDPGEMQDAFREWSLEAVRNSVAVAD
                     RVAPKVIIDTSYKIPTFPLPEEARGPDEYLEELARAGLERRLAPIAELLAAGRTKHPR
                     KDYDDRLEWELGVIRRLGFSGYFLIVWDFIKYAKDNGIPVGPGRGSAAGSLVAWTLGI
                     TDVDPLRYDLLFERFLNPDRVSMPDIDVDLCERRRGEVIEYVRHKYGKENVGQIITFN
                     SMKARAVIRDVGRALDVPLAEVNKLCAMIPANPGKPTTLSEARKSVKELSDTLAGNEG
                     YRRLFDLAERLEGVSRHAGVHAAGVLIAPRPLVEYLPLYKNNNDEITTQFEMKSVEKM
                     GLLKMDFLGLITLTILDDAVKSVVRRTGRSLDLGEIPLNDPEVFRLFSEGRTDGIFQF
                     ESSGMRDLLRRVQPSVFSDLAALNALYRPGALDAGTVEVYIERRRGKKFEYPLPQLEP
                     ILKDTYGVLVYQEQVMLAARAVAKYTPGEADLLRKAIGKKDEAALQAEGEKFIRKAVA
                     EGVPKKKADELWGLILPFGRYGFNKSHSVVYALLAYRTAWMKVHYPVEFMAAVLTAAS
                     GKSEDVVKYVNACREMKIPVLPPDVNASQLSFTPDGDAIRFGLGAVKGVGEGAVASIL
                     GARAADGPFVALTDFCCRVDLRLNNRKVIEALIRSGSFDSLTKSRATLAAGVDTALEV
                     AAAERRSRESGQGGLFADETAADHEDRFAELPEWSPDERIRYEKETLGFYITGHPLAR
                     FEEEIALFGDVTAEQAGERVDQNVRIVALLASLKKTQIKKGQNEGKMMAKGVLEDLTG
                     SVPVTIFAALLERVGSWLSEGTPVLVSGVVRSSSAPGAPEPEGEGAPVPVEIIAREIQ
                     ALEGMREAAATELCLATPLPGTLDDTLASVRELLESSPGSVPVYLDVRRAGQWAVRVR
                     TAGRLAVRPTPELTGGLERLLGPGAVRYVYDHS"
     gene            35216..36112
                     /locus_tag="PLP50_02450"
     CDS             35216..36112
                     /locus_tag="PLP50_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50439.1"
                     /translation="MTTAPVLPQGPLRTLARRAGEARARAYLVGGAVRDAALGHAPTD
                     LDVALEGPNSAVEEVVRRLGEDGFTCEARHARFGTATLRTPDGMRLDLATTREESYPV
                     PGALPVVHCGAPIERDLARRDFSIHAMARALGTRGPERALIDPFGGAGDLGLRRLRLL
                     HERSLADDPTRVLRAARYAARLGFSLDAGFDAAMQHALSSGAFANTSGDRLRRALEEA
                     LSEENRHVALEILLKLEVPVALVAGWEGRYLPPRGGLPPASSPSALWSALLAPAPEAL
                     RARIARRLAFPRALRAAARCGR"
     gene            36109..36840
                     /locus_tag="PLP50_02455"
     CDS             36109..36840
                     /locus_tag="PLP50_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50440.1"
                     /translation="MTRIAAVLLVSAALAAGGGAFGEPPSAPSPGSSSLAPEPTPRPD
                     DKTLVAALDVEQGDGKDRVSLYRDGTLALVRTYLGVRTVKKQVLAEEEVDVVRRVCSE
                     ALRVDVPEFRMDVLGPTKPRRFRIEIGREGDLPKVYVVDELARLPLVLGRARGALEEL
                     ISRFDETDGAHWQSWDPTAVKEGDVLVRRDDGLRYRVVRDDAFVRSLELLEAHRRLQR
                     LVVLREELPRMFHPPGTDGTEGSRR"
     gene            36837..37454
                     /gene="rsmD"
                     /locus_tag="PLP50_02460"
     CDS             36837..37454
                     /gene="rsmD"
                     /locus_tag="PLP50_02460"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPA50441.1"
                     /translation="MIRIVAGEYRGRRLGVGPGVRPTTERAREALFSILGDRVRGARV
                     LDAAAGSGALGFEALSRGASSAVFVEADRRVAKTLESNLALVGAGGRAVVVVRTVAAY
                     LRTDRPSGFDLVFFDPPWADPVANELEGLWSAVSPGGTFVLERRSGGGDPWPGPPRPP
                     MTRRYGATTLYLYARPATVETEPAGPADVPAGEAGSWIDPDPSGK"
     gene            37504..38670
                     /gene="metK"
                     /locus_tag="PLP50_02465"
     CDS             37504..38670
                     /gene="metK"
                     /locus_tag="PLP50_02465"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPA50442.1"
                     /translation="MALPRTLFTSESVTEGHPDKIADQISDGILDAILADDPDGRVAC
                     ETLVTTGTAFIAGEITTRTYVDIPQVVRETIRDVGYTRAKYGFDYQTCAVLSSIDKQS
                     PDIAMGVDPGGAGDQGLMFGFAVRETEELMPLPITLAHRLTMRLAEARKSHELEWLRP
                     DGKSQVTVEYEGSRPVRVDAVVVSTQHAETIPTLELREAIREEVIRRVVPASLIDERT
                     KYHINPTGRFVIGGPQGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNLVAAGLADRAEVQLAYAIGVADPVSVFVETFGTEKVDPALLPGLIRDHFRLT
                     PKGIIEMLDLRRPIYRKTASYGHFGRRLPEFTWERTDLAESLARAAGLAVPASA"
     gene            complement(38685..41018)
                     /locus_tag="PLP50_02470"
     CDS             complement(38685..41018)
                     /locus_tag="PLP50_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPA50443.1"
                     /translation="MKLTDRYVLREILAPTLVAFGVYTGFMLVRFLVQFSALLLQSTN
                     PLADVGRVLAFSAPHVVVLTIPVAFLLGILVGVGRLSQDSELVALRASGVDLLGLYRP
                     IALLAVLLALITGFVMVWVLPRTNQVVSGMRLQLSTFLIAQKIQPGVFSPEMAGRRIY
                     VEKASADRRTLEGLIVSDRSNPSDGERLTIARRGALELEQEEGRLWLRLEDAVTHHTA
                     PDARAYDRASYRTQRLLLDDTNPKARFENTRPDKQLREMTVPELIRRARTTPDLVLAR
                     LSWIEFHKKFSFPAACLVFGLVALPLGVVNRRGGRAAGFAISTAIVVGYYLLYAEGET
                     WTIRGSTTPLVAMWLPNALLAVIGLWGVSRVRRDRPFLDNRVGPALGGVIARAFPRSW
                     RTGNREGSSTGTAARRSPGAVGANSWLIDRYVAGRFLRILALVVTSILLLYVLFEYLE
                     ISDEIARVNPPLSAITGYFRAKLAPILVDVVPIAFAAAALISIAGLVRSAETTALLSS
                     GVSLFRTTASVLVLAVLAGAGTYLFAEMVVPDSAAEADRYKKILVGKPPAAASDAWRS
                     FLKGDSGRFFSAETWDPGSSSLTGVTLFRIDPSTYRLLSKSYAPKARILQGKGILLTG
                     GWTRTFSEDGASLFLRNSGPSFVDAPEAARAFLAGRADPRQMNSLELARFIRIRRTAG
                     ADVSAIATGLYQKSSVALAPLLLTLVGLPFAFRYGRRGAVAGIGIALLLGLVYLLLGA
                     ALVKGGETGSLPPLLAAWGTNVFFSLGAGWGLLGVRT"
     gene            complement(41332..42249)
                     /locus_tag="PLP50_02475"
     CDS             complement(41332..42249)
                     /locus_tag="PLP50_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPA50444.1"
                     /translation="MRHDPHFVDQLFKGEEISIGRKIALNLIEANPDQPRNMLGDLGD
                     LKASIEARGVLEPILVRPREDGRFTIISGERRFRAALEAGLAEIPCIVFDVSDAEVLE
                     IALIENLQRKDLSPLEEADGYQTLQEKHGYTHEQIARAVGKSRVTITETLSLARLPLR
                     VKDECRRADIRSKSFLLEVSRLPTEPAMLEAVSAFEGGTGVGRDAVRDVRRTARTETP
                     TFRSFRKAYELNFQPENAPYAVSVVFRGNAVERREIVETLRDLIRRLGNGELDFERDG
                     RFLKQEAKSATGSLASSSELLGPSKSDAE"
     gene            complement(42278..43033)
                     /locus_tag="PLP50_02480"
     CDS             complement(42278..43033)
                     /locus_tag="PLP50_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPA50445.1"
                     /translation="MIIAITNQKGGVGKTTTAINVAAALAQKNLRTLLVDLDPQGNST
                     MSYLDRRSIGGSMYEVLVNPEVTVSSIVRPTALPGLSIAPAKISLAKADSKLIGEIDG
                     HYRLKDKMTDVAADYDAVIIDTPPTLGMITVNAMVASTHIIIPIQSSYFALEGTDDLL
                     ETIEKIKSRPNPNLQILGVVITLHDRRTVLGRDIQKQIDTVFQGKIFKTTISKSVRLE
                     ESPAYRESIFTFAPRSTGASEYYSLTEEILARV"
     gene            complement(43149..43634)
                     /locus_tag="PLP50_02485"
     CDS             complement(43149..43634)
                     /locus_tag="PLP50_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPA50446.1"
                     /translation="MSVKMDVKALIVDPIANMPVVILRDADDRSFLPIWVGVFEANAI
                     ALQLEGVRTPRPMTHDLLRQTISQLDGAVERVVITKLHENTFYAEIHLRAGGRTVIVD
                     SRPSDAIALALRTASPVFVEESVLEKSQAQDDSSEAQSHERLRKWFEEADPESLGKYK
                     M"
     gene            complement(43681..45093)
                     /gene="miaB"
                     /locus_tag="PLP50_02490"
     CDS             complement(43681..45093)
                     /gene="miaB"
                     /locus_tag="PLP50_02490"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPA50447.1"
                     /translation="MAPVNDGRPTAPRDAETAARPRRVFVETWGCQMNELDSRRFVGL
                     MSREGWLESNSADDADLVLLNTCSVRDKAEQKVYDYLGRVALLKKSRPGLRLGVCGCV
                     AQQEGEEILRRSPAVDFVLGTGRIELLPAVVRRVEREGDRPVEVGFDLDEVAYTPGVV
                     ARTIAHRASITVIEGCNKHCTFCVVPTTRGRERSRRFSEVVEECRRLVGEGVVEIELL
                     GQTVNAYSDPESGAGLAELLRAISSIAGLRRLRFVTSHPRNFGDDLIAAMAECEPVCP
                     SLHLPFQSGSDSVLRRMKRQYTRAEYVDLAGRLRSAVPGLSLSTDIIVGFPGESEEDF
                     ASTIRLLDEVRFGAVFCFVYSPRPSTAAARWDADVPARVAQERLARLNDHQQELQLRA
                     NEAMVGQVLEVLVEGPDRTGKRVSGRSPFNHLVHIENAPGTPAGSFVQTRIEQGLANS
                     LLGRALRIERAEPDGPSLPA"
     gene            complement(45081..45776)
                     /locus_tag="PLP50_02495"
     CDS             complement(45081..45776)
                     /locus_tag="PLP50_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50448.1"
                     /translation="MKRSFTASLALLALLLGAPARLATAADTQPTPTPGAASKGKKKL
                     GGGSFGTPPRPTPTPGAGSGSLADVARKAQEEEAAAPRGGRRIVITNESLRRTEPAQG
                     SSTITITGTADKKPVARTRPTAVPTELPEYRDASGRTEAEWRARAEEVRSRAEEAEAA
                     AAAARSEVRRLENDFYAWSDGNYRERVIRPAWDQAKQKLKDLETAADEARSAVDGLEE
                     EARKSGTPPGWLR"
     gene            complement(45773..46141)
                     /locus_tag="PLP50_02500"
     CDS             complement(45773..46141)
                     /locus_tag="PLP50_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPA50449.1"
                     /translation="MKTTDRIVKKPWGSERIFAANGRYAGKLISILAGQTLSYQYHRT
                     KEETIHVLSGTLGMETESNGERYLLALAPGETFHVTPGTKHRMFADGEDVLVVEVSSP
                     ELDDVVRLEDRYGREGTTQP"
     gene            complement(46138..47925)
                     /gene="ptsP"
                     /locus_tag="PLP50_02505"
     CDS             complement(46138..47925)
                     /gene="ptsP"
                     /locus_tag="PLP50_02505"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPA50450.1"
                     /translation="MKRSLSLTGIPVSPGIAVGRAVVCIGRSDSTPRRDLDADEVPAE
                     LERLRLAVESAIREIGDMAGQVETRLGKEYAAIFQAHALFLRDPSFLGPIEKKVRLEK
                     VNAEWAVEVVADALAARLRDLPDKDLALRASDLDDIATILKRGLGEGSGAVDRLATLP
                     WDSCVLVADELTPSDAVRIPRDKVVAFVTERGGKTSHAAILARSFGLPAVVAVPKLLA
                     SVGEGDRVIVDGREGVLWREPSEDVLTLFRERQGRDATRELSLKERSLSGTATTRDGV
                     EVAIRANIELAREVPDVFEYGADGVGLFRSEFLYLSSAGVEFPDEATQAAIYRQVVSR
                     LAPRPVVIRTYDLGGKKGARHLSGAEENPVLGLRGVRLCFSHPEMFRTQLRALLSVAS
                     GGDLRILVPMVAGVEEVRRVRAFVEEARQELVERGIDVPDSVPIGAMIEVPSAAVTAD
                     LIAPEVDFLSLGTNDLIQYTLAADRANEAVSEIYRPQHPAILRLIARVQDAARAAGKP
                     LAICGEMAADPALFLLLLGLGIREFSMGPRSVPLLKDIARCASAADAARMARAALSLS
                     TPDDVSALLAREAEALLSGDPETRAAVNV"
     gene            complement(47955..48224)
                     /locus_tag="PLP50_02510"
     CDS             complement(47955..48224)
                     /locus_tag="PLP50_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPA50451.1"
                     /translation="MIEKTVVLKNRLGLHARAAAKLVHTAAHYSSRITLSKDGEDVDG
                     KSILGLLLLAAGKGTPIVVRTDGDDEAEAMRALTDLVDRRFDESE"
     gene            complement(48221..48679)
                     /locus_tag="PLP50_02515"
     CDS             complement(48221..48679)
                     /locus_tag="PLP50_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPA50452.1"
                     /translation="MKNPDAPVADAAGSVGILLLSHGPLADALRETVQVLEPQPGEPL
                     GALSLAWDEAPEHASERLQRAIADADRGRGVVLLTDMFGGTPSNLALAFLQKGRIEIV
                     TGVNLPMVVKARALAREGKEPGEMARTLVEKGRRSIVAAGELMETEKRTA"
     gene            complement(48676..49557)
                     /gene="rapZ"
                     /locus_tag="PLP50_02520"
     CDS             complement(48676..49557)
                     /gene="rapZ"
                     /locus_tag="PLP50_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPA50453.1"
                     /translation="MTGPRDDQRLLVVTGLSGSGKSYAARALEDIGYNTIDNLPLALL
                     RSFVEEAARGKGGPGRNAVVLDVRNPGFAEAFPPLLDSLRPVLPVTVVFLEADEPTIF
                     RRFSETRRPHPLAVDRTLAEAMATERSLLAEVKGKADLVLDTTAMTVHELRSVLAEHF
                     RSPGDPGVLAVSILSFGFKHGVPPSIDLCFDVRFLANPHFVPELRHRTGRDPNVARFI
                     EEGSATGPFYGRLLDFLLFCLPNYRRENRAYLTIGVGCTGGRHRSVYVAERLGRDLAA
                     AGYPVRVSHRDDNREGP"
     gene            complement(49554..50573)
                     /gene="hprK"
                     /locus_tag="PLP50_02525"
     CDS             complement(49554..50573)
                     /gene="hprK"
                     /locus_tag="PLP50_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPA50454.1"
                     /translation="MLPTPPETKPHVRAADLESPALAPLGLKLVSGRAGLDRPIEWPR
                     VQKPGLAIAGFLPYVRAQRVQILGESEFDYLRTLSSRVVKERFDAFTSLGMSCVIVTK
                     GIRPPAVLRRFCQQRDVPLFATPRLTSTVIEGLTAFLEESLAPRVTLHGVLVEVGGLG
                     TLLLGDSGVGKSECALALVQRGHRLVADDLVVVKRFHNDALVGSSAGVIRHHMELRGL
                     GIVNVPLLFGVAAVLEKHTVELVIRLSHWVEGVEYDRLGLDEESEAILGVPVPLVRMP
                     VATGRDVALLVEIAARNHLLKRRGYNAAREMADRVHEEIARRTGSTDRERRRGRRRPR
                     TGGGA"
     gene            complement(50581..51114)
                     /locus_tag="PLP50_02530"
     CDS             complement(50581..51114)
                     /locus_tag="PLP50_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HPA50455.1"
                     /translation="MKIEVTARRLTVDAKTKDTIAKRLEKLSKVLPREAEAKVVVRLE
                     KRGVLVEVTITARQRTWAAEAVAADQLTAAQSALERVEAQAKKTKARVKDEKKHTTSG
                     VRSPEVSLPLEKATAPPEKAPRTESYEARRMFDEDALHAFDRSKREVLVYRDPSDEQL
                     HVLYRRKDGTVVILTPR"
     gene            complement(51148..52755)
                     /gene="rpoN"
                     /locus_tag="PLP50_02535"
     CDS             complement(51148..52755)
                     /gene="rpoN"
                     /locus_tag="PLP50_02535"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPA50456.1"
                     /translation="MGLEQKLSLKLSQRLVMTPTLQQAIKLLQMTRLELHDVVNQEIV
                     ENPVLEEDEAAEVPAGAEVDEAVAARESAEAADAADPRERPAEGTDAGDAAALAWDDA
                     GGAAGEVAPPPPAVGTAEAEIPAEPTQESFEAIDLEAYFGDYLEGSSSTAPRMDEEGE
                     EYSLENRAEAPPGLDAHLAEQLGVSEAAPAVRAACEYLIGNLDADGYLRLTLEEVAAA
                     IPCTEADAAEALELLQSFDPPGVGARSLAECLLIQARVEGVESPLLEELVTNRLHDLA
                     TKAVPVLARQMSVPAEELQQAIDVLKRLDPKPGRRYDSTRTIYVEPDVQVVKVDDEYV
                     VLFNDDGLPRLRVSPFYRRLLSSSNGPLDGEGRSYLREKMRAAQWLMKSLDQRKRTIV
                     RVAESIVRKQRDYLEWGVAHLKPLILRDVADDIGMHESTVSRVVSNKWMATPKGLLPM
                     KFFFHSAISSSAGEDVSSLAVKGKIKALVDAEDASQPLSDARLSELLAREGIRIARRT
                     VAKYREELHIPSSSIRKVERGPAGSPD"
     gene            complement(52800..53537)
                     /gene="lptB"
                     /locus_tag="PLP50_02540"
     CDS             complement(52800..53537)
                     /gene="lptB"
                     /locus_tag="PLP50_02540"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPA50457.1"
                     /translation="MSEPRPRLAAEALKKSYRGRTVVDGVSIEVNGGEVVGLLGPNGA
                     GKTTTFSIVVGLVEPDDGHVLLDEEEITALPMFRRARLGVGYLPQEASVFRKMNALQN
                     LVAILETLPLSRNERLERAHELLDRFKLSHLAASVADTLSGGERRRLEIARALTLSPR
                     FLLLDEPFAGIDPITVLDLQHVIRELATTGIGILITDHNVRDTLAIVDRGYIINAGRI
                     FRTGSPLTLSEDPEVRRIYLGDRFRLD"
     gene            complement(53534..55543)
                     /locus_tag="PLP50_02545"
     CDS             complement(53534..55543)
                     /locus_tag="PLP50_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50458.1"
                     /translation="MRPGARRRLRQALGSLAFVVFPVAIVLSWRPAGARTDTTPAEVA
                     ETLLAESGGVRDRVRFEEFDYSETEGDSEVYRLRAAEAIAFADGADRTFRLKDVAFVT
                     RDAESGRTALLEAPRAEFVPSTKAFRVFDGVRVEGAGVDLAGRSFRYDPLTRLLVSEG
                     PVTAMRGALVARALGGAVESGVGIVRFRDDVRLGGKDDRGRRIALTADAVDLERGGGL
                     AARGNVVLKTDDLVLRGREVERRLAGDGDRVTARGDVVALLARGAEGELDAPIRAAGE
                     SLELERDAEAEPSRLGLTGAPARLDLPPAAGSGARQAVAESFEAKLSDGRLARVEVPS
                     SFRLEESAPPAQAGSARVPASRRLEAGRGQLAFGSDGRALETAVLEGGVVLSEGERTR
                     VTAPSATLRSADESAVFAGSGSEKARYEDETARVAADVLTWFRREQRIEGAGGVKTTF
                     RGREGSEVLGGGSDAPVFSESDFLRVRSDEKTVLLTGNVTAWQEENVLRGHSILLDDR
                     DRSLRAEGDVRAVLRRRRVDPKTKAATVETVTASGSVLTHRESDRLLRIEGSASVVSG
                     TWTMKADVTDVVLGPERTIDHAESRGNVVVEDRADGRRGEGAKATWDPRVDAVALEGR
                     PATALDGKGNRMTGARLRFQRGSGRVDVETGPGIPSQGVLRPEGT"
     gene            complement(55540..57300)
                     /gene="recJ"
                     /locus_tag="PLP50_02550"
     CDS             complement(55540..57300)
                     /gene="recJ"
                     /locus_tag="PLP50_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPA50459.1"
                     /translation="MTSAFPPPRVTRRLRPHDAGAAALLVRELGVAAPLARVLAARGF
                     SSPAEARAWLQPDPASLHDPFGMGGMDAAVARLADACRRGARVVVFGDYDCDGIGALA
                     ILTTTLKRLGADAVPFLPHRLRDGYGLRSETLRRVLDEHAPEGIVTVDCGITAVEPIR
                     EAVARGAFVIVTDHHLPPEELPTGAVLLNPRLPGCSYPFKELAGAGIAWKLSEALLTA
                     DGARVGLDGKAVRSWMASLAKVAALSTVADMVPLTGENRILVSWGLAGLAGPRSAGLT
                     ALLSRSSVRAGKSPSVHDVAFRIAPRLNASGRIDHAFRALELLTTADAVRAGQLADEI
                     EGANTERRRMQERVVAAALERLEKTFDPVRDALVVEAGSPEDGWHRGVLGIAASKVAQ
                     TVSRPVLLLSREDGRVGGSGRTWGRTPLHERIAPVARRHEAEFGGHHAAIGISVAANT
                     FEAFRDDAKETFARSRDEEEWSVVLEADAPLRASEVTPELVEHLGRLEPHGMGNPRPL
                     FLLRSLSWNGRGRPVGERGLRLAFEGEGVRLDAVGWTLGSISTGSRAGTWDVLANVAH
                     DSFLDRPALTVLDAVPAEAA"
     gene            complement(57297..58259)
                     /locus_tag="PLP50_02555"
     CDS             complement(57297..58259)
                     /locus_tag="PLP50_02555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPA50460.1"
                     /translation="MSSDHRFEEPLERLRARIDGLRESPGSPARDKAIRKISDRLVKL
                     SGEIYANLTPWQKTLVARHPARPFTLDYVRVLLRDFVELRGDRAFADDPAIVAGFGVL
                     GDRTVAIVGHQKGRDTKEKVRRNFGMPRPEGYRKALRVMKLAEKFQRPVLTFIDTPGA
                     YPGVESEERGVSEAIAVNLLEMARLRVPLIATVTGEGGSGGALGIGVTDAILMLEHSV
                     YSVISPEGCAAILWKDQAKAREAAEAMRMTAADCKALGIADEVIPEPPGGAHSDPVAT
                     IDAVGQALVRHLDRLAAIPVDALLEARYAKFRRMGAWEGPAASR"
     gene            complement(58294..60705)
                     /gene="priA"
                     /locus_tag="PLP50_02560"
     CDS             complement(58294..60705)
                     /gene="priA"
                     /locus_tag="PLP50_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPA50461.1"
                     /translation="MTSDDVARRIAVLLPTGFPGLATYLIPSEQPLPPAGARVAVPFG
                     PRLLTGLVVPGEPDPAPEGTVLREVLTVLDEEPFLSESFVGLLTRAADYYFAPPGELL
                     RAAVPARLLAAGTAAYVPTSRAVGAHPPEGLTGAVLTEVLTSGRATALELGERLGSRG
                     LGKAIRLLLDAGLVRIPSEELRAARPPTTRSFAARGSADDPRLARRPGQRKALATLAA
                     LGRPASGAELRAAGVSAGTLAALAKQSLVAVVEEERPADAAAHALPARATAPIEPTAA
                     QRAAIDAVAEALERGGEARFLLDGVTGSGKTEVYLAALERAIALGRQGILLVPEIALA
                     PGLIRRVVARFGSRVALLHSGLSDGERSAAWERGRRGEVDAVVGPRSAVFAPLPRLGL
                     IVVDEAHESSYKQAEAPRYDSRTVARVRAHGEGAVLLLGTATPSMEDEQAARDGRLPR
                     LVLPARPGSRERARVEVVDLRGEAARPGDHGRVLFAARTVELLRGAFERGEQAIVLLN
                     RRGFSPALLCRSCGHDFRCASCSVARTYHRRGERLLCHYCGDFVPRPRACPSCGSEVL
                     MPVGFGTERLAERFEELFPEVPYAVLDRDAAARRGGAVRVLADFESGAAQALLGTQMV
                     AKGHDFPNATVLAVLDADALLSFPDFRSAERTFQLVTQAAGRVGRGERPGIVAVQTAR
                     PEHEAIAAAVAQDHASFAEGELRFRRAFGYPPFSHLLLALWTARELPEAEAAARAGRV
                     AIAGLPKLRLLGPAPAPLERLKGLYRVQLLVRSDSREALAAAGELLRSLPEPPRLDRD
                     PQSLL"
     gene            complement(60710..61333)
                     /locus_tag="PLP50_02565"
     CDS             complement(60710..61333)
                     /locus_tag="PLP50_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50462.1"
                     /translation="MIARRVVGVMFLAAFAAFGTALVLSARARGGERLPGEVEKGRRG
                     PPPSGRTEAEEEAVGATWTSGVRPATDEDALESEARQELRDLYGAYRPYFVRGDFDFD
                     GRLDFAQAFVRREGPEPLFDVAVYFGEEGGGFGPAVWVDRGLLLAGGDLSIDRSVLVV
                     SEDVEQDVSRRWRWEPSAGRFVDVDAEAGEGDEAPWVEPDLRTGTTA"
     gene            complement(61330..62112)
                     /locus_tag="PLP50_02570"
     CDS             complement(61330..62112)
                     /locus_tag="PLP50_02570"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPA50463.1"
                     /translation="MPARRPRRNDALSAALGYFRDLGVRELHLAAPPAVAGPTSARPG
                     ARPETRDAVPSTRPASAKGPAVPALPDLDTLRATVAACQLCGLCRTRTQTVFADGTGR
                     SRIMFVGEAPGADEDATGVPFVGRAGQLLTKMIGAMGLDRADVYIANVLKCRPPGNRN
                     PEPAEIAACRPYLETQIEVVKPAVLVAMGKFAAQFLLETEEPITRLRGKWGMRRGIAV
                     MPTFHPSFLLRQPERKKEAWEDLQQVLRKLGLPVPGRKESGS"
     gene            complement(62076..63323)
                     /gene="coaBC"
                     /locus_tag="PLP50_02575"
     CDS             complement(62076..63323)
                     /gene="coaBC"
                     /locus_tag="PLP50_02575"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPA50464.1"
                     /translation="MSGGGFPAPRVLLGVSGGIAAYKAAEIVRLLVEKGAVVRVLMTP
                     AAQRFITPLTLSVLSKSPVVADLWDPASGAVDHIELARWGEVLAVAPATADVLAKMAR
                     GIGDEALSTYALAHRRAIVVAPAMNTWMWAHPATQENLRILRGRGAVVVDPDSGDLAC
                     GDVGPGRMAPPARIVEAVVGAARVSRSLQGRRVVVTAGPTREPIDPVRFLTNRSSGRM
                     GYALAREAEKRGAEVVLVSGPVAIDPPPNVKLLKVDRTAEMLEAVLAELPSSDALVMA
                     AAPADFQAEAFTPKKIKRAGGIPEIRLALAPDILAAVRAVRTPRQVVVAFAAETDDLA
                     GNARRKLLDKGADLLVANDVSRPGIGFDSEENEVLLLAAGASEPVAVPRAAKAVVSAT
                     ILERLAALLDERCPPAAPAETTR"
     gene            complement(63331..63657)
                     /gene="rpoZ"
                     /locus_tag="PLP50_02580"
     CDS             complement(63331..63657)
                     /gene="rpoZ"
                     /locus_tag="PLP50_02580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPA50465.1"
                     /translation="MTTPNDETGASPMTETPEETPDGTPPSPQIESKFRLVHIASRRA
                     EQLMLGARPKVETKHTKYSRIALDEVAGGHVKWQLGQRPIEGAVEGELETGAIEPIDP
                     LPLITP"
     gene            complement(63680..64306)
                     /gene="gmk"
                     /locus_tag="PLP50_02585"
     CDS             complement(63680..64306)
                     /gene="gmk"
                     /locus_tag="PLP50_02585"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPA50466.1"
                     /translation="MRPAGDLFIVSSPSGAGKTTLIRRILADPAFVGTIHFSVSHTTR
                     PARPGEVHGREYHFVSPEEFESLAARDGFLEHAVYDGHRYGTSRGEVEPRLAAGIDVV
                     LDIDVQGARQVRSRMPEVVKVFVFPPSRQVLEERLTARGHNTPESIARRLGAAARELA
                     EFGEYDYAIINDVLDAAVDELRSIVVARRSSRRRRQERLEAILRTFPA"
     gene            complement(64395..65255)
                     /locus_tag="PLP50_02590"
     CDS             complement(64395..65255)
                     /locus_tag="PLP50_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPA50467.1"
                     /translation="MTGFGRAQAALPDGTQLSITLRSVNHRFLDLSLKMRDDLGALEA
                     PLRKLVGATVARGHVDLSVRVSRPAGGAVTIDGATAERYAQAWGDLARERDLPRELTA
                     RDLLSLPGVVRIAEDEAGETLAATILETAGAALVDLDASRRREGEAVRSALAAILDRL
                     EDGIGRVANEREGLSERLLAQLRERVAKLASGVPLDEARLAQEIALLADRADITEEVD
                     RFRAHLVEARRLMSGEHASGKRLDVLAQELHREANTASQKARELPLTRVLLDLKSDVE
                     ALKEQIQNVE"
     gene            complement(65261..67150)
                     /locus_tag="PLP50_02595"
     CDS             complement(65261..67150)
                     /locus_tag="PLP50_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPA50468.1"
                     /translation="MRDFLRLARLFRPHARAALVAVLAMVGVAVFTTLVAYLFGPLFD
                     QVLTPGQSSAVKQELAADPAARGLGKALGAAESARKTPVIQWLDDRLLDLQSAVGVTE
                     ANRGVVLPVILLVAFVLKNLCAFAAEYRFNAVGLAFVRDVRRLVYERLLGQSGAFHAK
                     NPSGDLISRVTGDVDRIQSLFGTDLADLVQSIATLLGLSVLVVSRSPQLTLVALVVAP
                     AIVVPVVLIANRLRRISKAGRERMGDLTGVLSETLRGHRVVQAYGAEKYEADRFGEVN
                     ERTFRLLRKGAGVMALSSPLVETASVLAFLVLLGYAGKRIEAGTMTLGAFISFGVGLV
                     MMYQPFKRATRINLALQQALASARRLFEVLDAPVLVVDRPEAAPLRPFEREVRFEGVS
                     FAYPGGPDVLSGVDLVVPRGSVTALVGPSGAGKTTLANLLPRFMDVTAGRISIDGVDV
                     RDVTLSSLRGAMALVTQEVVLFDDTVRRNVAYGLADVPESRVRAALAAANAADFVDAL
                     PKGLDTPVGEAGGRLSGGQRQRLAIARALLKDPPILILDEATSALDTESERAVQAALE
                     RLMAGRTTLVIAHRLSTVRRADQIVVLDRGRLVERGRHAELLALGGVYRRLHDLAAFE
                     EEKTP"
     gene            complement(67147..68292)
                     /gene="lpxB"
                     /locus_tag="PLP50_02600"
     CDS             complement(67147..68292)
                     /gene="lpxB"
                     /locus_tag="PLP50_02600"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPA50469.1"
                     /translation="MRLLVSAGEVSGDQHGAALLGALSKRDPALEAFGLGGDRCAARG
                     MRLIAHQKELAIIGVVEALGKIRFAHRLMRELTREAVASKVDAAVLIDSPDFHLPLAR
                     RLARAGVPVVFYVSPQVWAWRSGRARTLARLGRGVLTLFRFEKRWYDARGLGERVTWV
                     GHPLVDEARRELATPAAPPPPGRRRVVLMPGSRAGEVRSLLPILRDAVALLQVRRSDL
                     EVVLVKADSVPDDLLRRIAGERLDRWSVVSGPHLSLLAASDVLLVASGTATVEGLLAG
                     IPMVVVYKVATISYLLGKLLVKVPSVAMANILSDPGDGTRAVPELIQGAATPERVAAE
                     VERFLDDASHAAEARRRLAEGREELGEPGAPERTAEAVLALLGRREA"
     gene            complement(68297..68488)
                     /locus_tag="PLP50_02605"
     CDS             complement(68297..68488)
                     /locus_tag="PLP50_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50470.1"
                     /translation="MSTTLFADQFLHLVLLATILSAFFALLWRDDGPSRRRFFTRMWT
                     AIVGGSLAAAWAMSLVGSR"
     gene            complement(68485..68862)
                     /locus_tag="PLP50_02610"
     CDS             complement(68485..68862)
                     /locus_tag="PLP50_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50471.1"
                     /translation="MPRPTSGRIVAALLTLLVPGLGHAWCGRRARGAAFFGLVGAAFV
                     LGLLLGGSPGPVPARGTGALLTAAASHAVVLPALLVRAFGLGPAEPSSPTFEVATTYL
                     VVAGVLNVLLALDAWDVAGERKP"
     gene            complement(68868..69371)
                     /locus_tag="PLP50_02615"
     CDS             complement(68868..69371)
                     /locus_tag="PLP50_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012123544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPA50472.1"
                     /translation="MNDVLFTPWRMSYLTGAPPSGAGGCLFCDLPGLGDAEALIVERG
                     RSVFAVLNRYPYSNGHVLVAPYAHRATLPELTREERIELIDLSARIEEALRAEYAPGG
                     LNAGLNLGKCAGAGVPGHLHMHLVPRWEGDTNFMTVVSGTRVVPEDLEVTFRRLRARL
                     GTPEGAS"
     gene            complement(69368..69736)
                     /locus_tag="PLP50_02620"
     CDS             complement(69368..69736)
                     /locus_tag="PLP50_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPA50473.1"
                     /translation="MERESQGVSRAEETTTKADLVEEVARTAQLTKKQAEAVVESVFQ
                     GIISSLRAGSKIELRGFGSFKLRQRGARIARNPKRSGVKVHVPPKLVPYFKPGKKLKE
                     LINATAPAPESEPGARAPEE"
     gene            complement(69807..>70607)
                     /locus_tag="PLP50_02625"
     CDS             complement(69807..>70607)
                     /locus_tag="PLP50_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPA50474.1"
                     /translation="KLLSVGDTVEAVIADVNPETRRLSLSLRATEPNPWEQLAEKYRI
                     GDRVQGVVRNLTDFGAFVEIEEGIDGLVHISDMSWGRRVKHPSEVLKKGDQIQAVLTA
                     IDVENRRISLSIKEFRPNEWEEFSREHQPGDVVDGQVAKVADFGVFVRLPLGLEGLMH
                     VSETDVLRGQKLSDVFSEGDPVRVRILRIEVDEQRIGLSMRDVPQPDPAELAAAAEAV
                     AAPEAEGEPEARAASDPAPEVAAPAEPVAEESEAASTEASAETSKTED"
BASE COUNT        10454 a        24398 c        25842 g         9813 t
ORIGIN      
        1 ctcggcggcg cggccacggg cgtcctcgtc tcgccgtggc tcctggcgtt ctcggtcttc
       61 ttcttcctct cgctcgcctt cgtgaagcgg gcctccgagc tcgtctcggt gcgggagcgc
      121 gggctcggct cggcggccgg ccgcgactgg cggacggtgg atggctccac ggtccccgtc
      181 ctcggcgcgg cgagcggcta cctctcggcg gtcgtcctcg cgatctacat ccacagcgac
      241 gtcacgaagc gcctctacgc gcacccgggc tggctgtggc tcctggtgcc gatcctcctc
      301 tactgggtga gccggctctg ggtcctcgtc gggcgggggg agatggacga cgacccggtc
      361 ctcttcgcgg cccgggacgc cgtcacgtgg gggctggcgg cggcgacggc cgcggcgctc
      421 ctcctcgccg cgctcgggcc gaccggcatt cccggcctga tggaataggc cggccggcgg
      481 cgcacggggc cgcggatttt cctttgaccc ctcgacgtgg acgggaatag tgtcccccgc
      541 gagatcaggg aagccgagac gccgcgcgac ggtgtcggaa ggagacggtg aatggtgcgc
      601 atccgaaccg ccccgacggt cctcctctgc cttgccctcc tgctcctctc cctcggggcc
      661 ttcgcccagt cggccgcgac gacgggcgcg atcgagggga ccgtcgcgga cgaggccgga
      721 ggggtcctcc cgggcgcccg cgtcgtcctc cggaacacgg cgacgaacta cgagacgacg
      781 gtcgcgaccg ccgcgaacgg acggttccgc gggatcctcc tgcctctggg gccctaccgg
      841 ctcaccgtca cgctcccggg cttcgcgaag gtcgtccgcg aggggctgaa cgtctcggtc
      901 ggccagtcgg tgaacgtcgc ggtcacgatg aggctcgccg ccaaggccga ggaggtcctc
      961 gtcaccgccg agaacccggt cgtcgagacg actcggcccg aggggacgac ccgcatcgac
     1021 acgctcgccg tccggtcgct gccgaacaac ggccggaact tcatggactt caccaagctc
     1081 acgccgggcg tctcgatcgt ccagggcccc gacggtgacg agctctcgat caacggccag
     1141 aaggggatcc agaacaacgt ctccgtggac ggggcggact tcaacaaccc gttcttcggc
     1201 gagcagcgcg ggggccagcg gcctgccttc acgttcaacc tcgacgcggt ccaggaggtc
     1261 gtcgtcgtgg ccgacggggc gaacgccgag tacggccgct cctcctcggg cttcgtgaac
     1321 gtcgtgacga agtcggggac gaaccagctc gccgggacgg tccacgtctt ctacaaggac
     1381 gactccctcg cctcggccgc caggcgcccg gacggctcct cggccgacga gcacgactcg
     1441 aaccaggtcc aggggggctt cacgctcggc ggtccggtcg ccacggaccg catcttcttc
     1501 ttcgcggccg tcgacgcgca gggcggctcc tcgacgaagc agacggaccc gagccgtatc
     1561 gagcagaggg tcgtcgacta cttcgcgagc ctcgggtacc ccgacgagaa cggctcgatc
     1621 gagcggggca acgacgcgtt cgtggcgctc gccaaggtcg actggctcct cggctcgaag
     1681 cacacggtga ccctccgcgg cacctacacg aagtcgagcc aggagaacgg gacgttcgac
     1741 gtcgactcct ggggccggag cgcgaacgcg accgaggaca actggtcgcg ctccctgacg
     1801 gggaccgcga tctcgaactt cggctcgatc ctgaacgagt tccgcttcca gctggcccgg
     1861 gaggaccggc cgcgccccta cgacggtccg aacatcgccg gccaggaccg gccgctcccc
     1921 gacacggcgt tcgacttcgg ccggggctac cggttcggca tgccgttctt catcccggtc
     1981 gactactacg acacgcgcat ccagctgaac gacaacgtga cggtcctgac gggagcgcac
     2041 gagctgaagg cgggcgtcga gctcaatcgc gtgaacgcgg cccaggtctt ccgcggattc
     2101 gcgaacggcc gttacatctt cagctcgacg gacggcttcc tgagctacgc gcggaacccg
     2161 agctacgtcg agtgctccga cggcgcctcg agcccggacg gcacctgccc cgtcgggacg
     2221 acggtcaccg ggcccgtcct cctcttcctc cagcaggcgg gagtcggcgg gctgacggcg
     2281 gaggaggcgg gcacgcaggc gatccgccag gacgagccgg ccgtcttcgt ccaggacaag
     2341 tggcagccga cgtcgaacct cacggtccag ctcggcctcc gctgggaggc gcagatccag
     2401 ccggacccga tcactccggc ggaggacgtc ttctacgccg ggttcatcgg gaagacgtcg
     2461 ttggggcagg agttcccgtc ggacgggaac atcccgtcgg accggagcat gtggcagcct
     2521 cgcgtgggga tcacgtggga cccgacgaac gacgggcgga ccgtcgtgag ggcgaacgcg
     2581 ggcctctact acgcgcgcat cccgggactc acgttcgcct cgacccgctc cacgaacggg
     2641 agccgggggc agagcctcta ccgcgacagc tcgctcacca gaatcctcgg ccccgtcccg
     2701 gcctacccga acctcatccc gcagtcggag gtcggctccc cgttccgccc cgatgtctac
     2761 gtcgtcgcca gagacttcca gaacccgcgg accgtcgcgg cgagcgtcgg catcgagcgc
     2821 gaggtgatga aggactacgg ggtcctcgtc aagtacaact acgcgaagac gacccacctg
     2881 acgcggttcg tcaaccgcaa cgacgcgctg ctcggctcgc cctggtcctc gggcctgaac
     2941 ggtggcccga acggcatcgg gaccctgacg gtcgtcgagt cgtccgcccg gagcctctac
     3001 caggggatca cgttcggcgt gagcaagagg tggtcgaacg actaccagtt ccagctgaac
     3061 tacaccctct cgtgggacga gtccgacgac gataacgaga gggacccgtt ctcgttccgc
     3121 tacgcgaagg tgactgacct cgacgccgaa tacggctact ccgaccgcga ccagcgccac
     3181 cgactgaacg gaatcttcgt ctggagggcg ccgggcgacg tgaacgtcaa cctccgctac
     3241 tcgtaccggt cggcgcagcc gaagtcgatc accctcgccg gcgccgacgc gaacacgccg
     3301 caggaccgga tcaaccccga cggcacggtc acgcagcgga acctcggccg gaaggacaac
     3361 cagttctcct cgctcgacgt gcgcctctcg cgctccttcg acctcggccc aatccaggtc
     3421 gagccgatcc tcgagtgctt caacgtcttc aactcgaaga acctgaagag gcccgaggtg
     3481 acgaacctga tcttcaactt cgacgggacc gtgcagagcg gcctcggcga cccgaggcag
     3541 gtgcagctcg gtctccgcct ggcgttctga ggcggtcgga gaggccggcg gccgcgtgct
     3601 agattccggg gagggcgccc ggcggagtca gcgggcgcct tcgcccctcc cggggccgga
     3661 gccagcaccg tgacagagac gacggcggcc cagccgctta cccggatcac gacgaccgcc
     3721 acgttcagcg ccgcgcaccg cctgcacaac ccgtcgcggg acgacgagtg gaaccgtgcg
     3781 accttcggca agtgcaacaa cccgcacggc cacggccaca cgtacaccgt cgaggtgacc
     3841 gtcgaggggt cgatcgaccc ggtcaccggg tgggtcctcg acttctcgca cctgaagcgg
     3901 atcgtcgacg agaaggtcgt ccggcgctgc gaccaccgga acctgaacgt cgacgtggac
     3961 ttcctcgcgg gggtcgtccc gacggccgag aacctcgccg tccgcttctg gaacgagctc
     4021 gcgccgcacg tcgcgccggc gcggctcgtg aagctctctc tccacgagac ggagcggaac
     4081 cgggtcgtct accggggcgg ggcctgatga gagccctcgt ctcgaacgac gacgggatcc
     4141 acagtcccgg gatcgaggcg ctcgagcgcg cggcgcgggc cgccggcttc gagacctggg
     4201 tcgtcgcccc cgaccgggag cagagcgcga cctcgcacgc gctgacgctc acccggcccc
     4261 tccgggtcgt gaagacggcg gagcgttcgt gggtcgtcga cggaacgccg acggactgcg
     4321 tgaacctcgc gatctgccgg atcctgaagg acgtccggcc cgacttcgtc ttctcgggca
     4381 tcaacctcgg gccgaacctc ggcgacgacg tcacctactc ggggaccgtc gcggcggcct
     4441 tcgaggggac gctcctcggc gtgccgtcca tcgcgttctc tctggactgc cggaaggacg
     4501 gcggcgacgg tccggatttc tcggaggcgg agcggatcgc ggaggaggtg atccggctgg
     4561 cgctctcccg accgatggcg gaggacgttc tctggaacgt gaacatcccg gaggggcgac
     4621 cgaccgggat ccgggcgacc cggatgggcc ggcgccggta cggcgagagc gtcgtggaga
     4681 aggtggaccc ccgcgggcgg ccctactact ggatcggggg ctctcacgtc gacacccact
     4741 ccgacgggag cgacctgacg accgtggccg agggactcgt ctcggtgacc cctcttcacc
     4801 tcgacctgac ggcgtaccgc gctctcgagg acctctccgc gcggctcgct gggagccgcg
     4861 cgaacggcgc ggaccgaagc taagatcccg gcgtgaggcc ggaggacccc gaggtcgcac
     4921 gcgccgcgcg ggcgggcgca gaggcgcggc gcgccatggt cgcgcggatc gtcgccgccg
     4981 gcctcctctc ggatcggcag gtcgtcgagg ccctcgcgtc cgtgccgagg cacctcttcg
     5041 tcccgaggga gctcgccggc gaggcgtacg gcgatcacgc ccttccgatc ggagcgggcc
     5101 agacgatcac ccaggcggcg aacgtcgggc gagcggcgga gctggcggcg atcccttcgg
     5161 gcggtcgcgt cctcgaggtg gggacgggct cgggctacca ggcggccgtt ctcgcccgtc
     5221 tcgcggggca ggtcttcacg ctcgaacgga tcccccagct cgcggccgag gcgcggcgcg
     5281 tcctcgccct tctcgggatc gggaacgtct ccgtgaaggt cttcgacggg acgtacggct
     5341 ggcgggagca cgcgccttac gacgcgatcg tcgtcgcggc cggggcgccc gaggtgccgg
     5401 caccgctcgt ctcgcagctc tcggcgacgg gacgcctcgt cgtgccggtc gggccgcccg
     5461 gccggcagcg tctcctggtc gtcaagaagc tgccgagcgg acggacgcgg acggaggacg
     5521 ccggagaggt cgcctacgtc ccgctcgtcg ggaagttcgg ctgggagaag gccccggcgg
     5581 agtcgcggcg atgagtggcg gcttcgagaa cgccgcccgc cggtggctcg tcgcggggcg
     5641 tgtccaggga gtcggcttcc gggccttcgc ggcgcggacg gcgcgcggcc tcggcctcgt
     5701 tggcggagcc tcgaacctgc ccgacggccg ggtcctggtc gtcgcggcag gggccgcgca
     5761 cgccctcgac aggctggaat cggctctctg ggaaggtccg cgtttcgccc gggtccagtc
     5821 cgtggaggcg gcgcccgccg cgccggacga ggtcctcgcg agcttcgacg cggagtttcg
     5881 gctcgaccgg tagaatccct cactccgatg tcgaaaagaa ggatcctcgt cgtggacgac
     5941 gacgcagacc tgcgggcgct cgtcgagggg cagctccgag ccgcagggta cgagccgacc
     6001 ggagcggcga acgggagcga gggagtggag cgggccgtgt ccgaccgccc cgacctcgtc
     6061 ctcctcgacg tcggtctccc gggcctctcc gggtgggagg tctgtgcgac gctccggagc
     6121 ctcgcggaga cctcctcgat tccgatcgtg atgctgacgg gccggtcgga gatccgggac
     6181 ttcgtgacgg gccggcaggc cggagcgacc gacttcgtca cgaagccgtt cgcgcgggcg
     6241 aagctcctcg agacggtcga gaacgccctg gcggggaagg acgggtcgtc gccggtgccg
     6301 tacgggccgg agctccccga gcgacgggcc cgcgggctcc tcctcgacgc gctcaccggg
     6361 ctcccggcca tatccgtggt ggtcgactcg cttcgcgagc gcctcctggg cgacgaggag
     6421 ttcggggtgc tcctcgtcga cgtcgacgcc gcaggcgcgc tggaggagca ctacggctgg
     6481 gaggtcctcg acgacgtcgt tcgcgaggcg gggaggtcgc tccggcggct cgtcggaacg
     6541 ctcttctcga cccgcgacct gctcgccgtc cgccgtccgg gctcgaccgc catggtggtg
     6601 ttcgtcggcc tccctgccgg cctcggcgaa ggggaagccg cgacgcgcct cgaccggaag
     6661 gcccggcagg tggcggagtc gctcggagcg cttctgggag agcggttcct cgggcgcgtc
     6721 caccgccgcc tccaggtgac ggccgcggcg tcccggctcc gatacagccc gcaggtgagg
     6781 ctcgagcgcc tcgtcgaacg ggccctcgcc gaggcctcgg ccgtggcctc gacgcgggag
     6841 ggggagcggg cggcggcgca gaaggcggag ttcgacgccc tccttcgcgt gggacggctc
     6901 gaggtggtct tccagccgat ccgggacctc gcgtcgatga aggtcatcgc gcgggaggcg
     6961 ctcacgcgtg gacccgaagg ctcgagcttc gagagcccgg aggtcctctt cgagtacgcg
     7021 gcgcgccacg ggcgcgtcct ctccctcgag caggcgtgcc tcgcgctctc ggcgacccgt
     7081 ttcgtcccgg tcgcggagga gctcctcttc gtcaacgtcg agacccacgt cgtcagcgag
     7141 ctcgcgcacg gggggctcga ggtcctgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnna cgtcgagacc cacgtcgtca gcgagctcgc gcacgggggg ctcgaggtcc
     7321 tgaacccgct cgtcgcgctc ggcccgtcgg tcgtcctcga gatcacggag cgcggggcga
     7381 tcgccgattt cgaggtcttt cggcgggggc tctccgcgct ccggcgcgcc ggcttccgga
     7441 tcgccctcga cgacgccggt tcgggccacg cgtcgctcca cgccctggcc gagcttcggc
     7501 ccgactacct caagatctcc cagttcctcg tctcggggct tcaccgagac gggatcaaga
     7561 gcgagatcgt ggcgatgctc gtccggctcg gggagcggat cggggccgtg acggtggcgg
     7621 aaggaatcga gaccgacgag gagctggccg ccgtgcgcga gctcggcgtc gcgctcgggc
     7681 agggcttcct cctcggccgt ccggaaggcg cgacccgccg ctgacgccgg cgtcgagcgc
     7741 ggactggtct ttcgggcgat gatccggccg aggagaggcc gatgacggag cacgaggacg
     7801 gcggcccgga gcttccagac ggattcgaga aggtcgaggc gaccgtctgc cgtcgcgggg
     7861 gagagctcct cgtcttcggc acgctcgagc ccgagggccc gttcgtcttc cggctgcagc
     7921 tcgcgaagaa ggtcgaggac ttcggcccgc ctccgcccgg gttcgagtgg acgggagagg
     7981 agcgcccacc gaagaagggg gagtggttct ggtcggagct gcgcgggcgc gccgttcagg
     8041 cgatcaagga cgaaacgggc gtcaaccagt tcggggagcc gacgggcggg agacggatcc
     8101 tccgtccggc ggggacggcc ggacggccga cgccgacccc gaagcacggg ctccggctcg
     8161 tccggaacga cgagggcccc gaagggtcag gctgacgcgg gcggaggcga gcccgccggt
     8221 ggctcgcccc cgtcctcggc gtgccgggtc acggccagat agacgcggag gtcgtcgagg
     8281 accgcctcga gagcggactc cttcgacgcg gcgaagaacg gctcgtcggt cacgaatccg
     8341 cccgggaccc accagggcac gaagagcgtc tcccactcgg tgtcgaggcc gccgtccgcg
     8401 tcgaccggcc ccgaggggtg gacgccgacc tcgaggaggc gccaggtccg gcaccggaac
     8461 tggcccgggt tcgccgcgtc ctcgaagacc tcgatccgga tgtctgcggg ctctccgtcg
     8521 acgagcgtct cgacctcggc cacttcggcg aggcggaaga cggcgaggtg aggtgagcct
     8581 gcgtgcatcg aacgcgaagt ctatccgccg cggagacggg gtgtggtggg cccggggaga
     8641 ctcgaactcc cgacacgcgg tttaggaaac cgctgctcta tccacctgag ctacgggccc
     8701 gtccggaggg cgacgccttc gggaggcggg agcggagtgt accggatcac tcctcgaacg
     8761 gcccgaccga gacgtgtttc acgaggagga cgcggttcgc gcggacggcc tggtccgcgc
     8821 cgagcgggag gaagttgtcc tcctgccggg cgatgtaccg cgcgcaggcg tcggtcgcca
     8881 ggatgacgtc gtcggactcg aacggcatca ggaactccct cgggtcggcg agcccctcga
     8941 cgacgagctg gaaggtgtga aggtgctttc gatccatctg cttttcgaga cctccgcggt
     9001 ccggccggtc gggccggtcg cggcatcgta tcctctgaga gcttgagcga gatcaccgac
     9061 aggatcgagg tgagccggga gacgccggcc tacccggccc gccacgacgt gagggacggc
     9121 cacctcgtcg tcgggggcga gcgagtcccg gtcgtcgtga agaggatccc gcgcgatttc
     9181 cgacagccag acggaaggac gcgcgccgag cggtctctcc ggatcgcccg ccaccttctc
     9241 gcccacgggc tcgacacgcc ggagccgctc gggatcgagg tgacacccga ggagagcgtc
     9301 ctcgtcgtcc gaaaggtcga gcgggccgtc gaggtgcggg cgttcttcct gcggcgaggc
     9361 gatccctccc tcgggactct cccgctccgc tcgagcttcg acgaggtcct cgtcgccctg
     9421 gcccgcttcg cgcgccggat gcacgactcg ggagtcttct tccgcgattt ctctgacggg
     9481 aacgtcctcg tcacggacgg ggaagaggga ccgcgcctct ggatcgtcga cctcgaccgc
     9541 gctcggattc gaaggccgcc gctcgcgacc ttcacccggc tcagggacct ctcccggccg
     9601 gggctgacgc gggcggacga ccggcggcgc ctcctcgagg cctactgggc gccgggaccg
     9661 gttccggccg gtgccgcggc gctccacgcg gccctccggt tccggctcct cgcctggaac
     9721 cggctgaagg ccgtcctcag accctggaga cgctgaggag ctatcctcgg cggacgtaaa
     9781 cccccgagcc gccgatcgcg tcccttcggt catgaggacg acctcgacga cggtccgggt
     9841 cctgaggttc gaggacgatc ctcgagggag gagcagcgac gtggcgaacg acctcccggg
     9901 cgagggggcg cccgcgccgg agcccggaat ccgggaacgc gaaaaggagc tcctcgatcg
     9961 ggtgcggggc ggcgagaccg acgccttcga gcacttcgtg aagacgtacc agcgaaaggt
    10021 cttccgcctc gtctacacgc tcgttcggaa cgcggcggac gcagacgccc tcacccagga
    10081 cgtcttcgtg aaagcctggc gggccgtcgc ggacttcaag ggcgaggcgg cgttcgagac
    10141 gtggctgaac cggatcgccg tcaacgccgt tcgggacgcg gcgcgccggc gccggccggt
    10201 cgtgtcgttc gccgagctgg ccgacgcgga ggggacgggc ggcgcggagc tgccgccgtc
    10261 gatggaaccg gtcgatgcca cgacgccgga gcgggaggcc cgctcgcggc agatccgccg
    10321 gcggatccgc gaggcgctcg acgccctctc tccccggcaa aaggcgatct tcgtgatgaa
    10381 gcactacgag gaccggacgg tcgcggagat cggcgccgcc atcggggtcg acgacgggac
    10441 cgtcaagtcg cacctcttcc gcgccgcgcg gaagctgcgg cagaagctgg aggacctgcg
    10501 gtgagcggac ggcatctgac ggaggacgag gtcgtgaacc gggtcttccc ggcccaggac
    10561 ggaacggcgc cgatcccgat ccaccaggcg gtctgtcccg aatgccagga gcgcgtcgcc
    10621 cgcctccgcg aagcgtttct cctggaccgc ggggctctga cgggggtcgt cgagtcgctc
    10681 ccgccagaat tctggacggc ccagcggaag gccgtgatgg gccgggtctc cgagctcgcg
    10741 gcgctggagg ccgaggagaa ggcaacgccg ttcccgaccc gcctcacccg atccgtcctc
    10801 cagaggccgg ccctcgcgtt cggcagtctc gccgccgccc tcacgctcgt cgccggcctc
    10861 accgtcctgc gcggggggga cgaccggccc gcggagccgg agcagacggt ggcggtagcc
    10921 gagggaaccg cggccctctc ggcagccgat tcggccgacg acgagcttct tcgagcggtg
    10981 gacgtggccc tgacgaccga gtcgccctac gagacggtcc tcccggaggc gatgtgatga
    11041 gacgaacgct cctccttccg gcgcttctcg gcgccgtcct cgcggcctcg ccggccccgg
    11101 cccagatgct cgagatgccg ccgggcaagt ggtggaagag gcccgcggtc gtcgaagcgc
    11161 tgaggctgac gcccgagcag cagcagcgcc tcgaggaggt cttctcgaag aaccgccgga
    11221 cgttcgtcga cctgaaggcc gacgtcgaca ggcggacgat cgacctcgag gaccttctcg
    11281 ccgcccggga ggtcgagccg cgccgggtgg ctgcggcctc cgaggcgctc gagcaggcga
    11341 gggcgcgcct cgggaaggca cggacgatga tggtcgtcga gatgaggggg gtcctgacgg
    11401 gtgaccagtg ggcgacgatc gtcgaccgcc gcgaggagtt ccgcgccgaa cgccgggccg
    11461 aggtccggcg ccggtttgcg gaccggcgga acggagggag gcccgggcgc cccatggcgc
    11521 gacccgggac cccggctcct tcggcgcctc cggacccgga cgaggggccg gacgtgccgg
    11581 acgagccgga accctagcgg gtgcccggcc ttcgccgagc cagcacgatc caccgggtcc
    11641 cctcgggctg gaacgagagc cagcccgagg cgtcggcaaa gagccggacc ccgagcccgg
    11701 ccacgcgcag ggccctgcgg acggcggcct ccctccaggc gatttcgcga tacagctcgc
    11761 ggacgcgtcg gaatcgaccc ggcgcgccct ccacgggcag gaaccagtcg cgcgtgacgg
    11821 tgccggcccc gctctccggg tcgaacgagc cgcgctcggc cgagagcccg ccccctgata
    11881 ggtgcgtgac cctgtggtgg tccgggaacg cctcgatcgt ctcgggggag ttcaggtcga
    11941 agaggacgtg cccgcccggg gcgagcgcct tcgcgattcc cgccagcgcc gggccgagct
    12001 cctcgtggcg gtcgagatgg ttcagaacgt cgaacgtggc gagcgcgaga tcgaacgtct
    12061 ctcccacccc gagggggagg gagaggaggt ccccgcgtcg cacgtcgatc cggagccgtt
    12121 cgcgccgggc ggcagctcgc agaggacgca ggaacgggac cgccgggtcg acggccacga
    12181 cgcgcgcgcc gcggcgggcc agctcgaggc tctgccagcc gctcccgcag ccgacgtcca
    12241 ggagcgtctc gaagcggacg cggtgcgccc ggaggatctc atcgcgcgcg gggccgagga
    12301 ggtcgcgcca gaggccgtag acctcgtcgt agtagccggc gaggaccgcg tagggggggc
    12361 ggcgcgccgt cgtcacggcg cggggctccc gagggccttc ggcgccccgc cgcgggcgga
    12421 ggcgagagcg agcgcgccga gcgcgacgag gccgggaagc gcgaggaaga gcgcgtaggg
    12481 aaggccgaag ccttccgcct ggaggcggta ctgaagggcc gtgacgcctc cgaggaggag
    12541 cgcggcgggg aggagggcga gcggccgcca gcgcgcgaag acgacgagcg ccagcgcgag
    12601 gaacccgcgg ccggcggtga cgccctcgac gaaggtgggc gagacggtca ggacgagcac
    12661 ggcacctccc atcgcgccga ggacgccgga cgcggcggcg gcgccgctcc tcacggcgac
    12721 gaccgagagg ccgagagagc gaagcgcctc gggattctct cccgcggccg agagccggag
    12781 gccggcgcgc gtccgggtga gccagagatg aaggacgacc ggcgcgagga agctcgcggc
    12841 gacggcgtcg ggaaggccca cgagaccccg gggaagcgtc gcgacctcga ggaccgagcc
    12901 ggtctcgccg gcgccgagac ggtaggcgaa cgccgtcgcg ccgagggcga ggaggttcca
    12961 gacggtgccg acgaggatcg ggtcggcccg ccgcacgacg gcgaagagcg cgaaaaggag
    13021 cccggcgccg agcccggcga gggccgccga gaggaggccg ccccaggccg gcaggccggc
    13081 gcgccctgcg gcgaaggcga cccacgcccc gaacagcatc gttccctcga ggccgatctg
    13141 gatcgagccg gcgcgctcga ggaggagctc gcccgtcgcg gcgaggagga gcggggccgc
    13201 gagcaggagg gcgatctcga tgaagcccgt catctctccc tccgttctcc ccgggcgagg
    13261 aggagggcga gcacggcgag ggccgggacg atcgaagcca gcgacgaagg gacgccggcg
    13321 tcgcgctgca gggcgctcga tccggcgccg agagcggcga agaggagcgc cgacgcggcc
    13381 ccgccgatcg tcgacatgcc gccaaggagg gccgcggcga tccccgtgta gccgactccg
    13441 gccgtgaacg ggtcgtagag ccgcccggtc agggcgagga cctcgagccc tccggcgagg
    13501 cccgcgaggg cgccggacga gaggaacgcc ccgacgcgca gccggacgtc cggcaggccg
    13561 gcggcccggg cggcgcgggg cgcctctccg accgcacgga gcgcgaggcc gatcggcgtc
    13621 cgcgcgagga cgaaggcggc ggctgctgcg aggaggagag cgaggaggac cgccgccgtc
    13681 gcccgcgacc cggcggccag cggcggaagg agggcggcct ccgcgagcgc gcggctctgc
    13741 ggataatcgc ccgagggatc gcggagcggg ccgcgcacga gccacgtcac cccggccgcc
    13801 gcgacgagat tgaggaggat cgtcgagagg acggccggga cctgccgccg ctcggccagc
    13861 caggcggccg gcgcggcctg aagcgccccg gcgagcgggg ccgcgaggag gacggccgcg
    13921 cgggccgcga ggggcgcgtc ggggaagagc ggtcccgcgg cggcggccgc ggcggcgccc
    13981 gcgaggaact gtccctcgag gccgatgttc aggaccccgg cgcggaacgc gacgacggcg
    14041 ccgagcgccg tcaggacgag cccggtcgag cgcgcgagcg tctctccgag cgcggccgac
    14101 gagccgagcg ctccccggag gagcgcgacg aacgcgtcgg ccggcgaggc gccggcggcg
    14161 aggacgagga gggccgcggc ggcgagcgtc ccggcggcga cggcggcgac ggcggccggc
    14221 ctcacggcgc gagccccgcc atcctccggc cgagctcggc gagcggcgtc gtcgccgcca
    14281 gcggagggga gagagcgccg cgatagacga cccggatcgg cgcgccgagc tcgagcgcct
    14341 cctcgggatc ggccgtgacc acgaggacgc ccgcccccgc gcgggccgcg tcgaggagcc
    14401 gtgcccggac gtccgaggag gaggcgaggt cgaggccgcg cgtcgggtgg acggcgacga
    14461 ggagacgggg cagcggctcg agctcccgcg cgaggaggag cttctgctgg ttgccgcccg
    14521 acagctcgcc cgccttcgcc caggggccgc gggcccggac cccgaagcgg cgaagccggt
    14581 cgtcggcagc ggcggcgagc gcggcgcggt cgatcacgaa acgtcccggc ggcctccgga
    14641 ggaagaggtt ctcggcgagc gagagccccg ggacgaggcc ttcggcccgg cggtcggccg
    14701 ggatgaacgc gccgccggcc gcgcgaaagg cgcgcgggtc gcccggcggc acgggccgcc
    14761 cggagagacg gatccgtccg cgggcgggcc gaacgccggc gatggcggaa ccgacgaggt
    14821 cggcgccgtt cccgtccacc gcgaggagcg tgagggcggc gccggcgggg acggcgaggg
    14881 acgcgggcgg gccggtcgcc ccggcgggca ggagctcgtc gacggcgaga gcgggcaccc
    14941 gctctccgtc cggtggatcg ggacggagcg caccggagcg ggacgagacc tcggctccgc
    15001 cgaggaggag cgctccgacc gcttcgggtg tcgtcgagga gacctcggcc tcgaggagcg
    15061 tcctcccgcg ggagaggagc gtcaggcggt ccgcgccctc gaagacctcg gccagccggt
    15121 gcgtgatgag gaggacggcg cgcccggtgg cgacccgcgc ccggagcgac cggaagaggg
    15181 cgctcgtctc ccgcggcgag aggacggcgg tcggctcgtc gaggacgagg acgcccgggt
    15241 ccgcctggag gatcccggcg accgcgatcc gctggcggac cccgaccgga agctccccga
    15301 cgcgcgcacc gggcggcccg agctcgagcc cgagccgctc ctcgagccgg gcgacgcgcg
    15361 cgcgacgcgc ccccgccgac tcgaagggcg gcgcggcggg gtcgaggagc gcgaggttgt
    15421 cggccaccgt cgcggcctcg acgagaaggt cgtgctgggg caggagggcg atgccggccc
    15481 gtcgcgcggc ccgcgcgtcc gccgcgggaa ggagcgcgcc gtccctccgg acctcgcccg
    15541 cgtcggcgcg gaggagtccg gcggcgatcg cgaccagggt cgacttcccg gccccgttct
    15601 ctccgaggag cgcgtggagc tcgcccgccc ggaccgtgag gcgggcgccg tcgagggccg
    15661 tcacggggcc gaacgccttc cggatcccgt tcagctcgag cgcggcgccg gggtcgctca
    15721 tcccgtcgtc gcccgcctct ccggggcgac cgtcagaagt tccctcgcgg gacgatgatc
    15781 gccccggaga cgatcttctc cttcgcccgg tccacctcgt cgacgacggg ggccgggacg
    15841 cgaccgagga gcgccgggtt ccagacgaac gagacgacgc cgctcgcgag gccgtagcgg
    15901 attggcgtgc cgtcgaaccg cccgtccttc acccggcgcg ccgcctcgac gaaggcgcgg
    15961 gggaggtcga tgacggccga agcgaggacg ctcggcgaga cggagttctg gtcacggttg
    16021 gcgccgaagg cgtagacgcc ccgctcggtc gccgcggcga agacgccgag cgcggcggcg
    16081 ttcgcgttgt ggaacaggaa gtcgctgccg ccgtcgatga gggcgagcgc ggccgtcttc
    16141 gcgccggcca cgtcctcgaa ggagccggtg aagaccgccg tcgccttcgc gcccgggttc
    16201 ccggccttca ggcccgcctc gaaggcgagg aacgtcgact tgatcgacgg gatctcgacg
    16261 ccgccgacca tcccggcctt cgccgtccgc gacatccggc cggccacgag gccgcagagg
    16321 tacgtcgcct gctcgagctc gaagacgagc ggcgcctggt tgcgcgacgt tcgcgagccg
    16381 gaggtcgtca ggaagacggt gcgcggatag tccttcgcga cgcgggccgc ggcgtcctgg
    16441 aactcgaagc cgtgcccgat gcagagcgcg gcgcccccgg cggcgtaggc ccggaactgc
    16501 tcctcgaact ccgacggcgt cttcacctgg acgtgggaga ccttcgcgcc gagctcccgc
    16561 tcgatcgcct tcagcccctc gaaggcgccg gcgttccacc cggcgtcgga gaccggcccg
    16621 ggcgtcagga gcgccacccg gaagccctcg gccggcgtcg cgttgggggt gcggccgcag
    16681 gccgcgagtg aggcgacgag cggcagcgcc aggagcgagg cgaggagtcg gcggcgcgtc
    16741 gtcatcggcg cgagtttagt ccgctcagac gacggagcgg cccgcgtcga ccgccagcac
    16801 ctggccggtg atcgagcggt tcgaggagag gaagagcgcg gccgagacga ggtcgtcgac
    16861 ggagacggcc cggccgaggg gcgtccgcgc gacgagacgg gcgagctcct cgggcggaag
    16921 ctcctccggg ggaaggacga ttcccggcgc gatcccgttc acccgcaccg acgggccgaa
    16981 ggccacggcg aggttccgca cgagcgcgtc gaccgccgcc ttcgagacgg cgtgggggac
    17041 gtggcgcggc caggccttcg tcgcggccac gtcggaaacg aggaggacgg cgccgccgga
    17101 gtcggcgagc gcggaggcgg ccgcgcgggc gaggaagaag gcggcgcgcg gcccgacgga
    17161 gaagacgtcg tgccagtcct cctccgtcac ctcggcgacc ggcttctcga gccacggcga
    17221 ggccgagtgg aggaggacgt cgagccggcc gaagcgtccg aggaccgact cgacgaggcc
    17281 ctggggcgcg cccgcggcgc ggaggtcggc ccggaacgcc tcggcgcggg cgccgaggcg
    17341 ggcgatctcg gtgaccgttc gggccgccgc cgccgggtcg gaccgatagt ggacggccac
    17401 gtcgtgcccg gcgcgcgcgg ccccctcggc gagggcccgg ccgagccggc gggacgcccc
    17461 ggtgacgagg gcgacgggtc tcatggcgcg aagtgtctca ccgctgcggt gccgtgtgcc
    17521 gtccctataa tgccgccacg caacacggcc gggccgggga gggtgagatg acgacgccgc
    17581 gcactctggt cgacgtattc cgcaacctcg gggcgacggc gaagccggac ctcctcctcc
    17641 acaagaagga cggtcgctgg gtcccgatct cgacggcgga gttcacggag cgggtgaagg
    17701 ccgtcgccgc ggcgctcggc acgctcggcg tcccggacgg aggagccgtc gtcctcctct
    17761 cggagaaccg cccggagtgg ccgacgctcg acttcgcctg ccagtgctac ggcgcggtcc
    17821 tcgttcccgt cttcccgacg atggtggccg accaggtcga gttcatcgtc cgggactgcg
    17881 gcgccacggc ggcgttcgcc tcgacgcccg agcaggcggc caagctcctg aaggcccagg
    17941 cgacgtgccc gaagctgaag cacgtcttcg tcttcgacca tgccggcggc ggagggaagc
    18001 cgttcggggc gctcgtcgaa gaaggccgga aggcgcacgc ggcctcgccg gccgtgttcg
    18061 acgagcgggc cgaacgccgg cagccggacg acctcgccac gatcatctac acctccggga
    18121 cgacggggga gccgaagggc gccatgctca tgcagagcaa cttcgtctcc aacatgctcg
    18181 ccggctgctc cctcctgccg ttcgactcga ccgccgtgac gatgtcgttc cttcccctcg
    18241 cgcacgtctt cgagcggctc gtggagtact gctactacca ccggggggcg acgatcgcgt
    18301 tcgccgagtc gatcgacaag ctgcgggaca acctcggcga ggtgaacccc cacatcttcg
    18361 gcgccgtccc ccgcgtctac gagaaggtct acgcccgcgt catggacaag gtcgacgccg
    18421 gctccccggc gaagcagcgc ctcttccgga ccgccatcgg ggtcgcgaag gaggtcctcc
    18481 gtctccggac ggagaagaag acccccggcg gcgccctcgc gctccagcac ttcctctacg
    18541 agcgcctcgt cttccggaag atccgggagg cgctcgggac ccggttccgg ttcgcgatct
    18601 ccggaggcgc gccgctcgcg aaggacctcg cggagttctt ctgggcgatc ggggtcgagg
    18661 tctacgaagg ctacggcctg accgagacga gccccgtcat cgccgtgaac tgcccgtcgg
    18721 cctggaagct cggcaccgtg gggagggtca tgccgggcgt ggaggtcagg atcgctgagg
    18781 acggcgagat cctgacgcgt ggcccgcacg tcatgaaggg ctactacggc cgtcccgacg
    18841 cgacggccga ggcgatcgac cccgacggct ggttccacac gggggacatc ggtgtcctcg
    18901 acgaggaggg cttcctccga atcacggacc ggaagaagga gatcatcgtc aactcgaacg
    18961 gcaagaacgt tgccccggcg ccgatcgaga gcgccctgaa gggggacccg ttcgtcgcgc
    19021 agccggtcgt catcggcgac cggcggaagt tcctctcctg cctcgtcgtc ccgaatttcg
    19081 agaagctccg cgagtgggcc gcgaagaacg gcctcggcgc cctctccgac gaagagctcg
    19141 tcgcccaccc gcgggtcgag cagatctacc aggggatcgt cgaccgctgg aacgagggga
    19201 agccgaacga gcagctcatc cgcgcgttca agctcctccc gagggagctg acgatcgaag
    19261 gcggcgagct gaccccgacg ctcaaggtga agcggcgggt cgtcgaccag aagttccgga
    19321 gcgtgatcga cgggctgtac aagggagact gaccgcccgg cgtgagggcc gggcccgccc
    19381 ggtggggcgg acccggccct cgagtcgtca tcggacgatg gggatcatct cgacgtagga
    19441 gccgtcaccg gtcccggcgc ccggaacgcc gccgtcgttg acgacggcgt acgcgaggaa
    19501 gggcgcggtc ccgtcgatcc gctccacctt cacgtagccc tgggcgacct tccacttcga
    19561 gaggacgccg ttcagctgca tccacgcccc ggctccgagg gtcacaggtt cggaggtcgc
    19621 gaccttccgg ccggtctcgc cgtcgaagac gctgtagcga agcgtgacgg ggccgaggtt
    19681 cgcggcggcg ttcagaagag cgaggttcga gcggttcgcg gcattctgga tcaggccgaa
    19741 gatccacgct tcgcccggag cggcgtccgg cgccggcgcc gggcagaaga ggccgtagcg
    19801 gcccgggccg ctgccggcgg tcgccgtgcg cgccccggcc cagccctcca ccggctcgcc
    19861 ggcggccgag aacgtcacgg cgagcgtgcc ggcgtagtcc ctcttcgcgg gcgtgaccac
    19921 gaggccgagc tgacgcatcg cggagacgac gttcgggagg atcttctgct cgccggccgc
    19981 caggtcgacc gtgaccccgc cgagggagta gccgcccgga ttcgcgagcg actcgatgaa
    20041 ccagagccgg gccttcacgg cccggaccga cgggttcgcg aggacgagct cggtcgtgaa
    20101 ggagccggtc tcgacgacga cggggacgag ccgggtccct ccgaggcccg cggccggagt
    20161 ggccgggacg acggagccgt cgtgggtcgt gttgtcgttg aagacggcgt aggcgtggaa
    20221 cggggcgctt ccggcgatcc gctcgacgag ggcgtagccc gcggtgaaac ccgcctcgcg
    20281 gaggacggag ttcagctgga gccactgccc ggcctccagg cggacgacgg ggaggacctg
    20341 tttcggaacc gcagccccct caccggagtg gagggtgacc ctcaggtcga ggcttcccgt
    20401 ctcgccgaga ttcgcgaggg cgacgtgcga tcggtcggcg gcgttctcgc ggaggccgaa
    20461 cagccagacc ttcgacgcgt tcgcgacggc ggggtcgacc gccgtcatgg aaaggccggc
    20521 gcggcccgcg ccggaggaga cggtcgtccg ggtcgaggcc gaaggggacc acccggcggc
    20581 gacgttctcg aacgtcacgc ggagcgtgcc gccctgagac gagccgtcgg acgggatctc
    20641 gagacctttc gtccggagcc aggcgaggag gtccgggacg attcgctgcc ggccgccctc
    20701 gagcgtctcg gagaccgtgc ctcctccgcg agccccgagc gacgtagcgg gggtatagcg
    20761 gagcgtgacc ttcaccgcag agggcgaggg attcgcgagc gtgagctcgg ttgcgtagcg
    20821 gttcccgccc ggaccggcga cgtcgaggac gatcggcgcg gtgaggacgg agctgaggcc
    20881 gcggacgggg acctcgacct gcccgggggc cgaggcgcac cccttcgcgt tccgcgtgac
    20941 gacgccgagg acgacgagcc ccgggtcccc cgcggagaac gtgatgcggc aggtgtctgc
    21001 gccgtccgtt atcgcgccgt tcgagatcgt ccaggtgcag gccagaccct cggctccggc
    21061 gacctcggcc acggcgccgg tctcgccgct ttcgaggctc gcgggggcct ggatgaccgg
    21121 gagcgggggc cgggcgctcg tgcggactcg aagggagccc gagacctcgc agccgccggc
    21181 cgcgcgcacg gtgacggaga gggtcgtgtc gcccgaggcg cccgcggtga agacgaccga
    21241 tcgggtgccc tggccggatt cgatcgtgcc gttcgtggcc gtccacaccc acgtcgtccc
    21301 gtcgccggcg gcggggaccg acgcgacgtg gccgcccgag ccggcgcaga cctcggcggg
    21361 cgccgtgatc gtggcggcgg aggcgtccgg gaagacgatg acccgggtcg atccggagcc
    21421 gggcacggtg caggtctcgt tcgagacgct cacgaccgag taggtctcgg tctggtatgg
    21481 cgtgacggtc cgcgtcgaac tcggacctgc gaccgtctcg acgacgccgt cgctccaggt
    21541 cagcgtgtag gggcccgggc cggagagctg ggccgtgacc gtcgcgctgc ccccgaggca
    21601 gatgaggacg ctcggcgtcg agacgaaggc tcgcggagcc tccacgacgg tgaccgcggc
    21661 gctcccggac gcggagccgg agcagctcgc gtcctgcacg gcggtcacgg tgtaggtcgt
    21721 ggaggttgcg gggctcacgc tgcgcgtcgc gagcatcgtc gcgatgccgg actggaccat
    21781 cccgtcggac caggtgagcg tgaacggagg cgtccccgag agcgccgcct ggagcgtgat
    21841 ggggcttccc gcgcagatcg tgccgccgcc ggagaccgtg gcagatggag cgggcgcgag
    21901 ggagaggacg tgcgtggagc tggctgtgca gccgctcgga agcgcgacgg tgacgccgag
    21961 ggtgacgctc gctcccgtgg gcgtgaatgt cacggacggc ccggacgtgc tccccgcgat
    22021 cgtcccgccc gcgatcgtcc acgcgtaggt cgcgccggct cccgcatcgg ggaccgaggc
    22081 gacgagaccg ctcgcgcccg cgcagtacga gcccgcggcc gcgatcgcgg cggacggaac
    22141 ggggttcacg gtgacggggg cgctccccgt cgcgacgcct ccggcgcagc cgttggcgtc
    22201 cgcgaggctc gtgattgtgt acgtcgtgct cgtcgcgggg gtgacgctcc ggctccccgg
    22261 accggcggcg ccgacggact ggaccgctcc gtcggaccac ctcaccgtga agggcggcgc
    22321 gcccgtgagg tcgaacgaga cggtggccgc ctcgcccgcg cagaccgtgg cgccgccggc
    22381 cacggtggcg gaggggggga gggagacggg gaggtcgcgg gaggccgtcg tgctgcacga
    22441 gccgatcgtc gcgatgacgg agacggtcac ggggttcgtc gtgccgacga acgacatgac
    22501 gttcgtcccc tgtccggaga cgatcgaccc gttcgtgacg gtccagttgt agagggcgcc
    22561 cgtcgtcgcg gggatggaga ccgtggccgt cgtgcccagg cagatccggg cgggtgccgt
    22621 gagcgtcgtg tccggcgtgg gggagaccgt gatggtggca gaaccgctcg ccgtgccggc
    22681 gcagaggccg tctgtcaccg tacgaatcgt gtaggtcgtc gtcgcggcgg gagtgacgtc
    22741 gcgggtcgcc gggctcgccg cgattccgtt ctgcgcaaag ccgtccgacc agacgaggtc
    22801 ccacggtccc gaccccgtaa gggccgctga gagcgtcgcg ctctctccga cgcatacggt
    22861 gccaccgccg ctgacgctgg ccgtcggcgc ggtgccgccg cagacgtacg tgaagccgtt
    22921 tcggattcgt ccgacgtctc cgtccgggtt ctcgacctcg acgtctacga gtccgttgac
    22981 gtgggggccg gtaatggcgt tgatcaaatt cgatgcgacg ctcgtgacga cggcggggat
    23041 cccgccgatg cggacgacgg cgccggtctg gaagcccgat ccggagatcg tgaccggcgt
    23101 cccgccgagg gaggggccgg tggtgggaga gatcgacacc ggtttcgcgc tcgacgtgag
    23161 cgagatcgcg gtgagggtac cgtcccacga ggcgacgaag agcttcccgt ccgcgatggc
    23221 aagctgccca tcgcgggctg cgaacgtcca gacggcctcg tgagtcgtca ggtcgatcgc
    23281 gtagacggtg ccgtgcgacg agacgaggac atgcgtgttg gtcacgatta tcggttgggt
    23341 aatcgcctgg tctgtgcccg tcgggggagg ggtccaggac cagacgagcg tgtgggtgga
    23401 ctcgtcgatt acggacaggc caccgttgtt cgcggcgtaa atccgccctc ctgcaacgct
    23461 cggctggccg gtgaagccct cattgagctg ccaaccaatc gtcccggccg tcgggttgaa
    23521 gctgatgagg cgaccgttgt ggatcgcgag caccccgtat gcgccgagca cgggcgcgag
    23581 gtccatgctc caaccgttcc agtcgaagtc cgggtccggg atgaagcccg gggttgagtc
    23641 cgttccgtcc aggagcttct tgatgtagag cccgggggcg ccctcgccga cgtaggcgta
    23701 ggcacgcgcc ccgtcgacag caggcgtcca ctgatcgtag aacgggagcg ccgcgaacca
    23761 gatctggtcg ccgttgaccc cgttgaaccc gtacattccg ccgtagtacc cgccgttgac
    23821 gtagaccctg ccggcagaga cggtcggcgc gtagtgtctc ggccactgct ccgcgacggg
    23881 agacttgaag atcgtggtgc ctgaggaccc gtcgagtgcc cagagccagg aatcggtgga
    23941 gtggtttccc gtctggaagt agacgcggcc gttcacgtag cttggggggt tgactgtggg
    24001 cctgttttcg aacgccctgg accagagcgt cgcgccatcg ctcgtcctga gcgcgaagag
    24061 ctgcgcgtcg tctgagtatc cctggagcgt cgcgtagacc ctcccgtctc ccgccgcgac
    24121 aggattgacg gggagagtgc cacccgggac cgtgccgagg gtcttcgtcc atcgaacgtt
    24181 gaaccactgt ggctcgagcg acaccgggac gtagcccgtg tgcgcggggt tcccctggaa
    24241 catcggccag ctctgggccg cagcggggcg gggattgaac agccagacgg ccaggacgag
    24301 aaaagttggg aggagaaggc gggagaagga ccggagagcg gtggacatgc gcacgcgaag
    24361 aagcctcctc gagtctgggg cggggcggga aaagatggtc ggaaaccgaa caaggggtcg
    24421 gttttgatgg ccttcgtcgc cgaagacgta ccgggatccg gcgcgtttcg acattcggga
    24481 ggccatcgag cgattgtacg ttcgatgtcg ctgcgctcgc gaatcggaaa ccgttctcaa
    24541 ctgcaccagc ggagggtgac tgtgcccgga ctgggagcag cgggccgcgc ggccggagcg
    24601 tccttgactc ccgctgcgcc gcgacatagg ctctgagtga tggctcattc agccgcgctc
    24661 ccggcgggct cgaaaccccg cacctcctcc ggctcgggcc ggcggagcct tcccgccgcc
    24721 gacccggaga agcggaagcg cctcctccac gccgcgatcc agacgttcgg gcgccggggc
    24781 tttcacgagg cgaagatcgc cgacatcgcc gcggaggcgg gcgtcgccga ggggaccgtc
    24841 tacctctact tccgcaacaa ggaagacctc ctgggctgcg tcttcgacga gacgatggac
    24901 gaggtcctcg cccaggcccg ggcgatccgc cacgcggagg gaaccgcagc cgagaaggcc
    24961 acgaagctcg tcgggctcca cttctccttc atcgggcagg accgcgacct cgcctcggtc
    25021 ttccagatcg agctccgccg gagcgcgcgg cacgtcgagc gcttctcgcg ggtcaagctc
    25081 tccgagtact tccgcctcct cgacggggtc ctcaaggagg ggatcgcctc gggcgagctc
    25141 cggaaagacc tcgaaccccg ggtcgcggtg aggatccttt tcggggccgc ggacgagatc
    25201 ctctcggaat ggctgctttc cggcgacgcg aagcccgtcg ccgacgctga gaagctcgtg
    25261 gggaccctgc tcaaggggtt cctcttctgc gagaacgacg cgaaggaacg gcggccggtc
    25321 cgccgcagga ggacgacccc ttgagccgag ccgcctacat cctcgacgga gcccggaccg
    25381 ccttcgtgaa ggcggggacc gacttcaagg ccgttaccgc cgtcgacctc tgccgcgccg
    25441 ccgcggtgga ggcgatggcc cgttccggcg tcgagcccgg ggagatcgac caggcgatct
    25501 tcgggaacat cgcgacgccc cccgacgccg cgaacatcgg tcgcgtcatc gccctccgcg
    25561 ccgggctccc gaaggagaag ccggcgcact ccgtctcgcg caactgcgcc tcggggatcg
    25621 agtcgatcgt ccaggcggct cggctcatcc agacgggcga ggccgacgtc gtcctcgccg
    25681 gcggcgccga gagcatgtcg ctcatcccgt tcctctaccg cgaggacgtg aaggccgtcc
    25741 tcgtggaggc cgggatggcg aagacggccg gcgcccggat cggcgcgttc aagaagatgc
    25801 cgtggaagca gctcctgaac ccggtcatcg ggatcaagct cggcctcacc gaccccgtct
    25861 gcggcctgaa catgggggag accgccgagc tcctcgcgaa ggaaggcggc ctgacgcggg
    25921 agatgcagga cgccttcgcg ctccgctcgc accagcgggc gcaggcggcg cgggagaggc
    25981 tcgccgagga gatcgtcgcg gtgccgctcc cgcccgcgta cgacaaggtc gcgacgctcg
    26041 acaacggcgt ccgcgagggg cagacgatgg aggccctcgc gaagctgcgg ccgttcttcg
    26101 accgcgccta cggctcggtg acggcgggga actcctcgca gatcacggac ggcgcggcgg
    26161 ccgtcgtcgt cgcctccgag gagttcgtcg cgaagcggaa gctcgcgccg ctcgcgcgcg
    26221 tcgccggttg gggcttcgcg gggctcgagc cggagcggat gggcctcggg ccgtcgcgct
    26281 ccacgcccgt cgcgctgaag cacgcggggg tgccgttcgg gaagatcggc ctcctggagc
    26341 tgaacgaggc gttcgcggcg caggtcctgg cgaacctcgc ggcgttcgag aaggacccct
    26401 cgctcggcgc ggtggacccg gagatcctga acccgaacgg cggggcgatc gccctggggc
    26461 acccggtcgg cgcgtccgcg acgcggctcg tcctgacgct cctccgggag atgaagcgcc
    26521 ggaaggtcgc gtacggcctc gcgacgctct gcatcggagg cggccaggga gccacgatcg
    26581 tcctcgaggg ggtggcgtga tggccgaagg gaagcacttc cgcctcgagg tcgagccgga
    26641 cggcctcgcg atcctcacgt tcgactcgcc gggcgagaag gtgaacaagt actcgacccc
    26701 ggtgatgctc gagctcgacg cgctcctcga cgccctcgcg aagcggacgg acgtgaaggc
    26761 gctcctcctc gtctcgggca agcccgacat cttcatcgcc ggggccgacg tgaacgagat
    26821 cgcgaaggtg acggaccccg cgacgatcgc ggacggcgtg cggaaagggc aggccgtcct
    26881 cggcaagctc gcgaacctgc cgttcccgac ggtcgccgcg gtcgaggggg cgtgcgtcgg
    26941 tggaggctgc gaaacgctcc tcgcgatgga ctggcgcctc gcgtcggact cgaggaagac
    27001 gcagatcggc ctccccgaga tcaagctcgg gatccttccg ggctggggcg ggacgacgcg
    27061 cctgccgcgc atcgccggcc tcgcgaccgc cctcgacgtc atcctcgcgg ggaaggtcgt
    27121 cgactcgaag agggcgaagc ggatggggct cgtcgacgag ctcgtcccgg cgccgatcct
    27181 cctcgacgtc gcgaagaggt tcgcgcgcgg gaaggtcggg acgcggaagc gctcgaacgc
    27241 cgctcccgac gggggcccgc tccgcgccgt ccccgcgaag gcgatggaga agctcctcga
    27301 gggggtcggg cggcccgtcg tcttccgcaa ggcccgcgcg gccgtcctga aggagacgca
    27361 cggccactac ccggccccgc tcgcggccct cgcggcgatc gagaagggat tcggccggcc
    27421 gctccgcgag gcgctcgagc tcgaggtcga ggcggcgggg aagctcgtcg gctcgcccga
    27481 gatgaagtcc ctcgtcggcc tcttcttccg gatggaagag gtcaagaagg agacgggcgt
    27541 cgcggccggc gtgacgccgc gcgcggcgaa gcggatcggc gtcctcggcg cgggagcgat
    27601 gggaggcggc atcgcccagc tcgcggccga caaggggtgg ccggtgcgga tgaaggacat
    27661 ccgccacgag gcgctcgccc tcggctacgc gcaggccgcg aagatctggc aggagaagct
    27721 gaagaagcgg cggatgacga aggtcgactt cgcctccagg atgtcgctcc tctccgggag
    27781 cctgggctgg gagggcttcg agacctgcga cgtgacgatc gaggcggtcc tcgagaagct
    27841 cgcggtcaag caggccgtcc tcaaggaatg ggaggcggtc gccgcccccg aggcgatctt
    27901 cgcctcgaac acctcgacgc tcccgatcac cgagatcgcg aaggccgcgg cgcgccccga
    27961 gcgggtcgtc gggatgcact tcttcaaccc ggtccacaag atgccgctcg tcgaggtcat
    28021 cttcggcgag aggacggcgc ccgaggtgac ggcgacgatc ttcgacctcg ccaagaagat
    28081 ggggaagaca ccggtcgtcg tgaaggacgc gccgggcttc ctcgtgaacc ggatcctcgc
    28141 gccgtacctc ggcgagggag tccgcctcct cctcgagggg gtcccgatgg aggcgatcga
    28201 caaggggatg cgggagttcg ggatgccggt cggcccgatc gagctgctcg acgacgtcgg
    28261 gatcgacgtc gccgcgaagg gtgccgagac gctctcggcc gcgtggcccg agcggatgcc
    28321 gatcgacccg gcgttcggga agctcgtgac ggggagccgc ctcgggcgga agacgaagaa
    28381 gggcttctac cgctacgagg gcgacaagcg cgtcgggccg gatccggccg cgtacgccga
    28441 cctcggcgtc gcctcgcccg gctccgcgcc gatctccgcc gaggcgctcg aggcgcggct
    28501 cgtctacccg atggtcaacg aggcggcgtt ctgcctcgcc gaggagatcg tcccctctcc
    28561 cgggaagctc gacctcgcga tgatcttcgg aatcggcttc ccgccgttcc ggggcggcct
    28621 ctgcgcccac gcggacgctc gcggcctcgc cgccgtcgtc gacgccctct cccgtctcgc
    28681 ctccgagaag ggcccccgct tcaaacccgc cccgctcctc gccgacctgg cgaagcaggg
    28741 gaagacgttc ttcgggaagg agtagccatg tcgaccgtca agacggacaa gaccgacaag
    28801 accgtgaagg ccgagaccga ggccccgtcg ttcctgaagg ccctctatca ggggcgcttc
    28861 gacgagtcgc tcgtcttccc ttacccggag attccggaag acacgaagga gctcgtcacg
    28921 gcgttcgcgg acgcctaccg cgacttcgac gccgcgaaca tcgactccga gaagatcgac
    28981 gccgaccact tcttcccgcg cgacgtcgtg aaggggctgg gcgacctcgg cgcgatgggg
    29041 atggcgatcc ccgaggagta cggcgggagc ggcttcagcg ccgccgcgta ctgcaagatg
    29101 atggaggtca tcgggccgct cgacgcctcg gccgccatcg tcatcggcgc ccaccagtcg
    29161 atcggcatca aacccctcct cctcttcggg accgaggagc agaagaggaa gtggcttccc
    29221 gatctcgcgg tcgcgaagat ggtcggcgcc ttctgcctca ccgagccgga ggcgggctcc
    29281 gacgcggcct cgctgaagac gaccgccgtc tacgacccgg cgaccgacga gtacgtcctg
    29341 accgggacga agcagtggat cagcaatggc ggcttcgcgc ggttcttcac cgtcttcgcg
    29401 cgcgtccccg aggcgggcgt cgaggcgggc tcgcacaagg agatcgcctg tttcgcggtc
    29461 gttgccaacg aggacgggac gctctccggc ctctcccgcg gcgtcgagga gaagaagctc
    29521 ggcctctgcg gctcctcgac ctgccagatc atcctcgacg gctgccgggt gccggcgttc
    29581 aacctgatcg gcggcaaggg gaacggcttc cgggtcgccg tcgaaactct gaacaccggt
    29641 cgcacgtccc tcggcgcggg cgccgtcggc ggctgcaagc agatgatcaa gctggccgtc
    29701 gagcacgcga cgcagcgaaa gcagttcaag acccggatcg cggacttcga gatgatccgg
    29761 gagaagatct ccgagatgac ggcgcacacc tacgtccttg agtcgatggt ctacatgacc
    29821 gccggcctcg tcgacaagaa ggtggactac tcgcttgaag gcgcgtgctg caaggtctgg
    29881 gggacggagc tcctctggaa gacgatcaac gagtcgctcc agatcgcggg cggaaacggc
    29941 ttcatgaacg agtacccgta cgggcggggc ctgcgcgaca gccgcatcaa catgatcttc
    30001 gaggggacga acgagatcct ccgcctcttc atcgcgctca ccgggatgaa ggaggcgggc
    30061 gacgtcctga aggacttcca gggggcgctc agccacccgg tctcgcagta cggcgtcctc
    30121 gtggaccacg cgaagatgtg gatgcgcggc accgtgacga gctacaagct cgacaaggtc
    30181 cacccctcgc tcgcccccga ggccgaccag gtgggcaaat acgcggccgt gctgcgcaac
    30241 gcctgcgaga cgatggtcta ccggaaccgg aagaacgtcg tgaaccggca gtacctcctc
    30301 caccggatcg ccgacgtcgt catggacctc tacgcccaga tcgccacgct ctcgcgcgtg
    30361 acggcggcca tcgagaagaa gggggaggag aaggcgaagc cggagatcga gatcgtccgc
    30421 tggttctcgt tccaggcgcg gcaccggatg gtcgcgaacc tgaaggctct cgacaagcac
    30481 aaggacgccg aggcgacggc catcagcaac gtggtctacg aggcgggtgg ctatcccttc
    30541 gacctctggg gctggtgagc cgcgggaggg gggccgggcg cgtccggctc ccctccgcgt
    30601 ccctccggct tcgcgcacgc cgaaacggcg cagacgtcgc ggcggcgtga acgcgcctcg
    30661 tgtcgtcacg gtcccggtgc cgacaccgtt ccccgtcggc cccgtcaaca cgcacctcgt
    30721 cctcggcgag ccgctcacga tcgtcgacac gggtccgctc accgaggacg cctgggacgc
    30781 gctccgcgcg gggctcgcgc gccacggcgt gagcgtcggc gacgtcgagc gcgtcctcct
    30841 gacgcacggg caccaggatc acttcggcct cgcggcccgc atcgagcggg aggggcgggc
    30901 acgggtcctc ggcgggcggc tcgaccggga gcatttccgg ggccgtcgga atcgccggct
    30961 cctcctcgac gcgctcacgc gtgcgggttt cggggggctg gagcggctca cgatggtggc
    31021 gatgacggcc gccgtcgacc atttcgccga gccgctctcc gcctgggacg agctggccgg
    31081 cggtgaggtc ctcccggggg acggatggtc ggtcgtcgtc cgctcggtac ccggccacac
    31141 gcccgggagc ctgacgttcg aggtcccgga agcgggcatc ctcttcaccg gagacacggt
    31201 cctccgcgac atcaccccga acgccatcgt cgacgaggac ccggagcggc cgggagagac
    31261 cttccggagc gtctcgcgct acttcgcgtc gctcgactcc atcgaggcgg ggaagtcgcg
    31321 cctcctcacc ggccacgggg gccggatcgt cggcttcgcg gctcatcgcg cgaagctcga
    31381 ggggcgctat gcgttccgcg tgggtcagat cgagcgggcg ctttcgggaa ggccgaaatc
    31441 cgtggccgac ctctgccgcc gcatcttccc gaccgtgcgc ccgatggatc tcttcctcgc
    31501 ctactccgag gtcctcggct tcctgatgta cctcgaggat ctcggacggg ccgagcgcgt
    31561 cccgtgccgt ccggtcgagc gctggacggg ccggtgagcg gcgcggccgg atgcgacgcc
    31621 ggcccctcgc cccacgagga aggaccgggc gagcatcgga tggctagaat ccgcgtggca
    31681 tgtcgtccga cttcgtccac ctgcacctcc acacccagta ctccctgctc gacggggcga
    31741 acaggacgaa ggagctgatc gagcacgtcc agaagatcgg gatgagcgcg gtcgccgtga
    31801 cggatcacgg gaacatgttc ggggccttcg acgtccagat agaggcggtc aagaagggga
    31861 tcaagccgat cctcggcgtc gaggcttaca tctcgccggg atcgcgccgc gaccgcgaga
    31921 ccgtgaagac ggtcgacggc gaagggaaca gctaccacct gacgctgctc gccgagagcc
    31981 cggagggcta ccggaacctc tcctatctcg tctccgaggc cttcctgagc ggcttctact
    32041 acaagccccg gatggactgg gagctcctcg agaagcacca cgcgggcctg atcgccctct
    32101 cggggtgcgt caagtcggag gtctcgcagc ggctcctcgc gggcgacttc gccggcgcga
    32161 aggagacggc gctgaggtac gaggcgctgt tcgggaagga ccactactac atcgagctga
    32221 tggaccacgg gctcccgatc cagcggcagc tgatgccgga gctcctgcgc ctctcacgcg
    32281 agacggggat cccggtggtg gcgacgaacg acgcccacta ccttcgccgg gtcgactccg
    32341 aggcgcagga cgtcctcctc tgcatcggga tggggaagac ggtgaacgac tcccggcgga
    32401 tgaagttcta caactcggag ttctacgtga aggaccccgg ggagatgcag gatgcgttcc
    32461 gggagtggtc gctcgaggcg gtgcggaact ccgtggcggt cgccgaccgc gtcgcgccca
    32521 aggtcatcat cgacacgagc tacaagatcc cgacgttccc gctgcccgag gaggcgcgcg
    32581 gccccgacga gtacctcgaa gagctcgcgc gggccggcct ggagcggcgc ctcgcgccga
    32641 tcgccgagct cctcgcggcg ggccggacga agcacccgcg gaaggactac gacgaccggc
    32701 tcgagtggga gctgggcgtc atccggaggc tggggttctc gggctacttc ctgatcgtct
    32761 gggacttcat caagtacgcg aaagacaacg gaatccccgt cggaccgggg cgcgggagcg
    32821 ccgcgggctc actggtcgcg tggacgctcg ggatcaccga cgtcgacccg ctccggtacg
    32881 acctcctgtt cgagcggttc ctcaaccccg accgcgtctc gatgcccgac atcgacgtcg
    32941 atctctgcga gcgccgccgg ggagaggtca tcgagtacgt ccggcacaag tacggcaagg
    33001 agaacgtcgg gcagatcatc acgttcaact cgatgaaggc gcgcgccgtc atccgcgacg
    33061 tcgggcgcgc cctggacgtc cccctcgccg aggtgaacaa gctctgcgcg atgatcccgg
    33121 cgaacccggg gaagccgacg acgctgtcgg aggcgcggaa gagcgtcaag gagctctccg
    33181 acacgctcgc ggggaacgag ggctaccggc gtctcttcga cctggccgag cggctcgagg
    33241 gggtctcgcg ccacgccggc gtccacgcgg ccggcgtgct catcgccccg cgcccgctcg
    33301 tcgagtacct gccgctctac aagaacaaca acgacgagat cacgacccag ttcgagatga
    33361 agtccgtgga gaagatgggt ctcctgaaga tggacttcct cgggctgatc accctcacga
    33421 tcctcgacga cgccgtcaag tccgtcgtga ggagaacggg gaggagcctc gacctcgggg
    33481 agatcccgct gaacgacccg gaggtcttca gactcttctc cgaaggtcgg acggacggga
    33541 tcttccagtt cgagtcgtcg gggatgcggg acctcctccg gcgtgtccag ccgagcgtct
    33601 tcagcgacct ggccgcgctc aacgctctct accgtcccgg cgcgctcgac gccgggacgg
    33661 tggaggtcta catcgagagg cgccgcggaa agaagttcga atatcccctc ccgcagctcg
    33721 agccgatcct gaaggacacg tacggggtcc tcgtctacca ggagcaggtc atgctcgcgg
    33781 cgcgggccgt cgcgaagtac acacctggcg aagcggacct gctgcggaag gcgatcggga
    33841 agaaggacga ggctgcgctc caggccgagg gcgagaagtt catccgaaag gccgttgcgg
    33901 aaggtgtgcc gaagaagaag gccgacgagc tctgggggct catccttccg ttcggacggt
    33961 acgggttcaa caagtcgcac tccgtggtct acgccctcct cgcgtaccgg accgcctgga
    34021 tgaaggtcca ttatccggtc gagttcatgg cggcggtgct gacggcggcc tcgggaaagt
    34081 cggaggacgt cgtgaagtac gtgaacgcgt gccgcgagat gaagatcccg gtgctcccgc
    34141 cggacgtgaa cgcctcgcag ctctcgttca cgccggacgg agatgcgatc cgcttcgggc
    34201 tgggagccgt gaagggggtc ggcgagggag ccgtggcctc gatcctcggg gcgcgcgccg
    34261 cggacgggcc gttcgtggcg ctcacggact tctgctgtcg cgtcgacctc cggctgaaca
    34321 acaggaaggt gatcgaggcg ctgatccgat cgggctcgtt cgactccctg acgaaaagcc
    34381 gtgcgaccct cgcggccgga gtcgacaccg ccctggaggt cgcggcggcc gagcggcggt
    34441 cccgcgagtc cgggcaggga gggctcttcg ccgacgagac cgccgccgac cacgaggacc
    34501 gtttcgccga gctcccggag tggtcgccgg acgagcggat ccgctacgag aaggagactc
    34561 tcggcttcta catcacgggc catcccctcg cgcggttcga ggaggagatc gccctcttcg
    34621 gcgacgtgac ggccgaacag gctggcgagc gggtcgacca gaacgtgcgg atcgtcgcgc
    34681 tcctcgcgtc gctcaagaag acgcagatca agaagggcca gaacgaggga aagatgatgg
    34741 cgaagggggt gctcgaggac ctcaccggtt cggtgccggt gacgatcttc gccgcgctcc
    34801 tcgagcgtgt cggctcgtgg ctgtcggagg ggaccccggt cctcgtctcg ggtgtcgtcc
    34861 ggtcgtcttc ggctcccgga gcgcccgagc cggaagggga gggggcgccc gtgccggtcg
    34921 agatcatcgc ccgcgagatc caggcgctcg aggggatgag agaggcggcg gcgacggagc
    34981 tctgcctggc gacgccgcta ccgggaacgc tggacgacac gctcgcttcg gtgcgggagc
    35041 tcctcgagtc gtctccgggc tcggtgcccg tctatctcga cgttcggcgg gcggggcagt
    35101 gggcggtgcg ggtccggacc gcggggcggc tcgccgtccg gccgacaccg gagctgacgg
    35161 gcggactgga gcgcctgctc gggccgggcg ccgtccggta cgtctacgac cactcgtgac
    35221 gaccgctcct gtcctgccgc agggccctct ccgcacgctg gcccgtcggg cgggagaggc
    35281 gcgcgccagg gcctatctcg tcggcggcgc ggtgcgggac gcggccctcg gacacgcgcc
    35341 gacggacctg gacgtggcgc tcgagggccc gaactcggcg gtcgaggagg tcgtgcgccg
    35401 gctcggggag gacggattca cctgcgaggc gcgtcacgcg agattcggca cggcgacact
    35461 ccggacgccg gacggaatgc ggctcgatct ggcgacgacg cgcgaggaga gctatcccgt
    35521 tcccggggcg ctccccgtcg tgcactgcgg ggccccgatc gagcgcgacc tggcgcggag
    35581 ggacttctcg atccacgcga tggcgcgcgc gctcggcacg cgcgggcccg agagagcgtt
    35641 gatcgacccg ttcggcggcg ccggcgatct tggcctccgg cgtctccgcc tcctgcacga
    35701 gaggtcgctc gcggacgacc cgacccgggt actccgcgcg gcgcgctacg ccgctcggct
    35761 cggcttctct ctcgacgccg gtttcgatgc tgcgatgcag cacgcccttt cctccggcgc
    35821 cttcgcgaac acctcaggtg atcgcctccg tcgcgcgctc gaggaggcgc tctcggagga
    35881 gaatcgacac gtggcgctgg agatcctgct gaagctcgag gttccggtgg cgctcgtcgc
    35941 gggatgggaa ggaaggtatc tcccaccgcg aggaggcctg ccgccggctt cttcgccgtc
    36001 ggctctctgg agcgcgcttc tcgcaccggc cccggaggcg ttgcgcgcgc gcatcgcacg
    36061 gcgtctggcg ttccctcgcg cgctccgggc cgcggcgagg tgcgggagat gacacggatt
    36121 gcggccgtcc tcctcgtctc ggcggctctc gcggcagggg gcggcgcttt cggagagccg
    36181 ccgtcggcgc cgtctcccgg atcgtcctcg ctagcgccgg aaccgacgcc gcggccggac
    36241 gacaagacgc tggtcgccgc gctcgacgtg gagcagggag acgggaaaga ccgcgtctcc
    36301 ctctaccgcg acgggacgct cgccctcgtc cggacctatc tcggggtccg gacggtgaag
    36361 aagcaggtcc tcgccgagga ggaggtcgac gtcgtccgga gggtctgctc cgaagcgctc
    36421 cgggtcgacg tgccggagtt ccggatggac gtcctcggcc cgacgaagcc gcgccgcttc
    36481 cggatcgaga tcggccgaga gggcgacctc ccgaaggtct acgtcgtcga cgagctcgca
    36541 cgtctgccgc tggtcctcgg tcgggcgcgc ggcgcgctcg aggagctcat ttcccgtttc
    36601 gacgagaccg atggggccca ctggcagtcc tgggacccga ccgccgtgaa ggagggggac
    36661 gtcctcgtcc gccgcgacga cggactgcga tatcgcgtcg ttcgggacga cgcgttcgtg
    36721 cggagcctcg agctcctcga ggcgcaccgc aggctccaga ggctcgtcgt gctgcgagag
    36781 gagctgcccc ggatgttcca tccgcccggg acggacggta cggaggggag ccggcgttga
    36841 tccgaatcgt cgcaggcgaa tatcggggcc ggcgactcgg cgtcggcccg ggcgttcgac
    36901 cgacgaccga gagggcccgg gaagccctct tcagcattct cggggaccgg gtccgcggcg
    36961 cgcgcgtcct cgacgcggcg gccggctccg gagcgctcgg cttcgaggcg ctgtcgcgtg
    37021 gcgcctcgtc ggcggtcttc gtggaggcgg atcgtcgcgt cgcgaagacg ctcgaatcga
    37081 acctggctct ggtcggggcc ggagggcggg ccgtcgtggt cgtccgcacg gtcgcggcgt
    37141 acctccggac cgaccggccc tccgggttcg acctcgtctt cttcgacccg ccctgggcgg
    37201 acccggtcgc gaacgagctc gaagggctct ggagcgccgt ctcacccggc gggacgttcg
    37261 tcctcgagag gaggagcggt ggcggtgatc cgtggccggg gccgccgcgg cccccgatga
    37321 cgaggcgcta cggagcgacg accctctacc tctacgcgcg gccggcgacg gtggagaccg
    37381 agcccgcggg ccccgccgac gtgccggcgg gggaggccgg ctcgtggatt gaccccgatc
    37441 cctccggcaa atagactttt cgatttcgtg tccttcgacg ggacacgcag gggaggccgc
    37501 gagatggcgc tgccgcgaac gctcttcacg tccgagtcgg tcaccgaggg ccacccggac
    37561 aagatcgcag accagatctc cgacgggatc ctcgacgcga tcctcgccga cgaccccgac
    37621 ggccgggtcg cctgcgagac gctcgtgacg acgggtaccg cgttcatcgc gggcgagatc
    37681 acgacgcgta cctacgtcga cattccgcag gtcgtccgcg agacgatccg cgacgtcggc
    37741 tacacgcggg ccaagtacgg cttcgactat cagacgtgcg ccgtcctctc gtcgatcgac
    37801 aagcagtcgc ccgacatcgc gatgggcgtg gaccccgggg gtgcggggga ccaggggctg
    37861 atgttcggct tcgcggtccg ggagaccgag gagctgatgc ctctccccat cacgctcgcc
    37921 caccggctca cgatgcgcct cgcggaggcc cggaagtcgc acgagctcga atggctccgg
    37981 cccgacggca agagccaggt cacggtggag tacgagggaa gccgcccggt gcgcgtcgac
    38041 gccgtcgtgg tctcgaccca gcacgcggag accatcccga ccctcgagct ccgcgaggcg
    38101 atccgcgagg aggtgatccg gcgggtcgtc cccgcgtccc tcatcgacga gaggacgaag
    38161 taccacatca acccgacggg ccgtttcgtc atcggcgggc cgcagggcga cacggggctc
    38221 accggccgga agatcatcgt cgacacgtac ggcgggatgg gacgtcacgg cgggggagcc
    38281 ttctccggaa aggacccgac gaaggtcgat cggtccgcct cctacatggc tcgctacatc
    38341 gcgaagaacc tggtggccgc aggcctcgcc gaccgggccg aggtccagct cgcttacgcg
    38401 atcggcgtgg cggatcccgt gtcggtcttc gtcgagacct tcgggacgga gaaggtcgat
    38461 ccggccctcc tcccgggcct catccgcgac catttccggc tcacgccgaa ggggatcatc
    38521 gagatgctcg acctccggcg gccgatctac cggaagacgg cgtcgtacgg gcacttcggg
    38581 cgccggctgc ccgagttcac ctgggagcgg accgacctcg cggaatctct ggcgagggcc
    38641 gcggggctgg ccgtccccgc gagcgcctga ttggcccggg gcggtcaggt ccggacgccg
    38701 agcaggcccc agcccgcccc gagcgagaag aagacgttgg ttccccacgc tgcgagcagc
    38761 ggcggtaggg agcccgtctc tcctcccttg accagggccg cgccgaggag aaggtagacg
    38821 aggccgagga ggagggcgat cccgattccg gcgacggcgc cacggcggcc gtagcggaac
    38881 gcgaagggga gtccgacgag ggtcaggagg aggggcgcca gggcgacgga gctcttctgg
    38941 tagagaccgg tcgcgatcgc cgagacgtcg gcgccggcgg tcctccggat ccggatgaag
    39001 cgagcaagct cgaggctgtt catctggcgg gggtcggccc ggccggcgag gaaagcgcga
    39061 gcggcctcag gagcatcgac gaacgagggc cctgaattcc gaaggaagag ggatgcgccg
    39121 tcttccgaga acgtgcgcgt ccagcccccg gtcaggagga ttcccttgcc ctggaggatc
    39181 cgggctttgg gcgcgtacga cttgctgaga agccggtacg tggacgggtc gatccggaac
    39241 agtgtgacgc ccgtgagcga cgaggagccc gggtcccagg tctcggccga gaagaagcgt
    39301 ccggaatcgc ccttgaggaa agaccgccag gcatcggatg ccgccgccgg gggcttgccg
    39361 accaggatct tcttgtaccg gtcggcctcg gccgccgaat cgggaacgac catctcggcg
    39421 aagaggtagg tcccggcgcc ggcgagaacc gcgaggacaa ggacggaggc cgtggtacgg
    39481 aagagggaaa cgccgctcga gaggagcgcg gtcgtctccg ccgaacggac gaggccggcg
    39541 atggagatga gggcggcggc cgcgaaagcg atcgggacga cgtcgacgag gatcggcgcg
    39601 agcttcgccc ggaagtagcc ggtgatcgcg gagagcggag ggttcacgcg cgcaatctcg
    39661 tcggagatct cgaggtactc gaagaggacg tataggagga ggatcgacgt gacgacgagt
    39721 gcaaggattc gcaggaagcg ccctgcgacg taacgatcga tcagccacga gttggcgccg
    39781 acagcccccg gcgaccgccg tgcggccgtt cccgtcgagc tcccttcgcg attcccggtt
    39841 ctccaggaac gcggaaaggc tcgcgcgatc acgcccccga gggcgggtcc gacgcggttg
    39901 tccaggaagg gccggtcgcg tcggaccctg gagacccccc agagcccgat cacggcgagg
    39961 agggcgttcg ggagccacat cgccacgagc ggcgtcgtgc ttccccggat cgtccaggtc
    40021 tcgccctcgg catagagaag gtagtagccg acgacgatgg ccgtcgagat ggcgaagcct
    40081 gcggcccggc caccccgacg gttgacgacg ccgaggggga gcgcgacgag cccgaagacg
    40141 aggcacgcgg cggggaagga gaacttcttg tggaactcga tccaggagag ccgtgcgagc
    40201 accaggtcgg gggtcgttcg cgcccgccgg atcagctccg gcacggtcat ctcacggagc
    40261 tgcttgtcgg gtcgggtgtt ctcgaaacgc gccttcgggt tcgtgtcgtc gaggaggagc
    40321 ctctgcgtcc gatagctggc ccggtcgtag gcgcgggcat cgggagccgt gtggtgggtg
    40381 acggcgtcct ccagccgaag ccagagccgt ccttcctcct gctcgagctc gagcgcaccg
    40441 cgccgggcga tcgtgaggcg ctctccgtcg gacggattcg accggtcgga gacgatcagc
    40501 ccctcgagcg tcctgcggtc ggccgaggcc ttctcgacgt agatccggcg tcccgccatt
    40561 tctgggctga aaaccccggg ctggatcttc tgcgcgataa ggaaggtgga tagctggagc
    40621 cgcatcccgg agacgacctg gttcgtccgg ggaagaaccc agaccatgac gaaaccggtg
    40681 atgagggcga ggagcacggc gagcagcgcg atcggtcggt agaggccgag gaggtcgacc
    40741 ccggacgcgc gcagggcgac gagctccgag tcttggctga gccgcccgac gccgacgagg
    40801 atcccgagaa ggaacgccac cggaatggtc aggacgacga cgtggggggc cgagaacgcg
    40861 aggacccgac cgacgtccgc gagcgggttc gtgctctgga ggaggagcgc gctgaactgg
    40921 acgaggaacc ggacgagcat gaagccggtg tagacgccga atgccacgag cgtcggcgcg
    40981 aggatctctc ttagaacgta gcgatcggtc agcttcatct ttggaggagc gtcctcaggc
    41041 gtggacgccc ggaggggatt ggagagcgca attctagccc gttccccttc cgcgctgcgt
    41101 ccgataagaa atattatggt aaaattcgag acctagagcc cgaacaacgt aaggcgaagt
    41161 gtatcgcaga ggtcctccgg tccagcgcgg ccaaccagcc gccgtgccag tcgagccctc
    41221 cccccaagac cccgggtcaa gctggagtcc cggcgaggac cttcgggcga accgccgtcg
    41281 tcgcgccccg cctcgactgg ctttggcgct ggctgacggc gacggcttcg cctactcggc
    41341 gtcggacttg gacggcccca gcaattcgct ggaggaagcg aggctgccgg tcgcgctctt
    41401 cgcttcctgc ttcaggaaac ggccgtctct ttcgaagtcg agctcgccgt tcccgagtct
    41461 tcggatcagg tcccgaaggg tttcgacgat ctcccggcgc tcgaccgcgt tgccgcggaa
    41521 aacgacgctg acggcgtacg gagcgttctc cggttggaag ttgagctcgt aggccttccg
    41581 aaaggagcgg aatgtcggag tctccgtgcg agcggtccgt cggacatcgc ggacagcgtc
    41641 ccgcccgact cctgtgcccc cctcaaaggc ggaaacggct tcgagcatcg ccggctcggt
    41701 tggcaaccgg ctcacctcga gaaggaagga cttcgatcga atgtcggcgc gccgacattc
    41761 gtccttcacc cgcagcggca gtcgagccag ggagagcgtc tcggtgatcg tgacgcgcga
    41821 cttccccacg gctcgcgcga tctgctcgtg ggtgtagccg tgcttctcct gaagcgtctg
    41881 gtagccgtcg gcctcctcga gaggactgag gtcctttcgc tggaggttct cgatcaacgc
    41941 gatttcgagg acctcggcgt cgctcacgtc gaacacgatg caagggatct cggccagtcc
    42001 ggcctccagg gcggcccgga atcgccgctc gccggagatg atcgtgaaac gcccgtcctc
    42061 tcgcggacgg accaaaatcg gctcgagaac tcccctggct tcgatggagg ccttgaggtc
    42121 tccgaggtct ccgagcatat tccggggctg atccgggttc gcctcgatca ggttcagggc
    42181 gatcttcctg ccgatggaga tctcctcacc tttgaagagc tgatcgacga agtgcgggtc
    42241 atgccgcatg cgagacgtct cgggaaggcc gcgacgctta gacacgggcg agaatctcct
    42301 cggtcaggct gtagtactcg gacgcgccgg tcgaccgggg cgcgaacgtg aagatggatt
    42361 cccgataggc cggagactct tcgagccgca cagacttcga gatcgtcgtc ttgaagatct
    42421 tcccctggaa gacggtgtcg atctgcttct gaatatcacg tcccaggacg gttcgccggt
    42481 cgtgtagcgt gatgacgacg ccgaggatct gaaggttcgg attcggacga ctcttgatct
    42541 tctcgatggt ttcgagaagg tcgtccgtcc cctccagggc gaaataggag gactggatcg
    42601 ggatgatgat gtgggtcgag gcgaccatcg cgttgaccgt gatcatcccg agcgtcgggg
    42661 gcgtatcgat gatgacggcg tcgtagtccg cggcgacgtc cgtcatcttg tctttcaggc
    42721 ggtagtgtcc gtcgatctct ccgatgagct tcgagtcggc cttggcgaga gagatcttcg
    42781 ccggggcgat cgagagcccg ggaagcgccg tcggtctcac gatggaagag acggtgacct
    42841 cggggttcac gagcacttcg tacatcgagc cgccgatgga acggcggtcg aggtacgaca
    42901 tcgtagagtt gccctgcggg tcgagatcga cgaggagggt gcggagattt ttctgggcga
    42961 gcgcggcggc gacgttgatc gcggtcgtcg tctttccgac tccgcccttc tggttcgtga
    43021 tcgcgatgat catgggggac ggcgaggata cgggctgcga cgcccgttct caaggctgga
    43081 tgtaagtcgt ttggatcgag gcggctggcg gctcactcct ggcggcgaac tcgggcttcc
    43141 ccgcttcgct acatcttgta cttgccgagc gactccgggt cggcctcttc gaaccacttc
    43201 cgaagacgct cgtggctctg cgcctcggag gaatcgtcct gcgcctgact cttctccagg
    43261 actgactcct cgacgaagac gggagaagcg gtcctcaagg cgagggcgat cgcgtcggac
    43321 gggcggctgt cgacgatgac ggttcggccc ccggcgcgaa ggtggatctc ggcgtagaag
    43381 gtgttctcgt ggagcttcgt gatgacgacg cgctcgaccg ctccgtcgag ctggctgatc
    43441 gtctgccgga ggaggtcgtg cgtcatcgga cggggcgtcc ggaccccttc gagctggagg
    43501 gcgatcgcgt tggcctcgaa gacaccgacc cagatcggaa ggaagctccg atcgtcagcg
    43561 tcgcgaagga tgacgacggg catgttggcg atcggatcga cgatcagtgc cttgacgtcc
    43621 atcttgacgc tcacatcgag ctccttccct tgtcgaatct gcgttcccga agccgtggat
    43681 tcaagccggc aggctcggac cgtccggctc ggctcgctcg atgcgaagcg cgcggccgag
    43741 aagggagttc gcgaggccct gttcgatcct ggtctggacg aaggaacccg cgggcgtccc
    43801 cggtgcgttc tcgatgtgga cgagatggtt gaagggagat cgcccggaga ccctcttccc
    43861 cgtccgatcg ggtccctcca cgaggacctc gaggacttgt ccgaccatag cctcgttcgc
    43921 ccgaagctga agctcctgct gatggtcgtt cagtcgggcg agccgttcct gagcgacccg
    43981 cgccgggacg tccgcgtccc accgtgcggc ggccgtcgaa gggcggggag agtagacgaa
    44041 acagaagacc gcgccgaaac gcacctcgtc gaggaggcgg atcgtcgagg cgaagtcctc
    44101 ttcgctctcg ccggggaacc cgacgatgat gtcggtcgaa agcgagaggc cgggcaccgc
    44161 cgaccggagc cggccggcga ggtcgacgta ctcggcacgg gtgtactgac gcttcatccg
    44221 gcgcaggacc gagtcactcc cggactggaa cggaaggtgg agcgaagggc agaccggctc
    44281 gcactcggcc atcgcggcga tcaggtcgtc accgaagttg cgtggatggg aggtgacgaa
    44341 acggagcctc cggagaccgg cgatcgaaga gatcgcccgg aggagctccg ccaggccggc
    44401 accgctctcg gggtcggaat aggcgttgac cgtctgtccg agaagctcga tctcgacgac
    44461 gccctctccg acgaggcgcc ggcactcctc gacgacctcg gagaagcgac ggctccgttc
    44521 ccgaccccgt gtcgtcggga cgacgcagaa cgtgcagtgc ttgttgcatc cctcgatgac
    44581 cgtgatcgag gctcgatgcg cgatggtgcg ggcgacgact cccggcgtgt aggcgacctc
    44641 gtccaggtcg aacccgactt cgacaggacg gtctccttcc cgctcgaccc gtcggacgac
    44701 ggccggaagc agctcgattc gtccggtccc gagcacgaag tcgaccgccg gcgagcggcg
    44761 gagaatctcc tccccttcct gctgtgccac gcaaccgcag accccgaggc gcaatcccgg
    44821 tcgggatttc ttgagaaggg cgacccgtcc gaggtagtcg tagacttttt gctcggcctt
    44881 atcgcgaacg gagcacgtat tgagcaggac gaggtcggcg tcgtccgccg aattcgactc
    44941 gagccatccc tcccgcgaca tgagtccgac gaatcggcgg ctgtcgagct cgttcatctg
    45001 acagccccag gtctcgacga agacccttcg cgggcgcgcg gccgtttccg cgtcgcgagg
    45061 ggccgtcggc cggccgtcgt tcaccggagc catccgggtg gggttccgct cttccgagcc
    45121 tcttcctcga ggccgtcgac cgcgcttcgc gcctcgtctg cggctgtctc gaggtccttc
    45181 agcttctgct tcgcctggtc ccaggcgggt cggatgacgc gctcccgata gttgccgtcg
    45241 ctccaggcat agaagtcgtt ctcgagcctc cgcacttcgg acctcgccgc ggcggccgcc
    45301 gcctccgcct cttccgcccg agaccggacc tcctcggctc gcgcccgcca ctccgcttcg
    45361 gtgcggccgc tggcatcgcg atattccggt agttcggtcg gtaccgcggt gggcctcgtt
    45421 cgggccaccg gcttcttgtc ggccgtaccc gtgatggtaa tggtcgagct cccctgcgcc
    45481 ggttccgttc ggcgcaggct ctcgttcgtg atgacgatcc gccttccgcc gcgaggggcc
    45541 gctgcctcct cctcttgcgc ttttcttgcg acatcggcaa gggatccgga accggctccc
    45601 ggcgtcggcg tcggcctggg cggagtcccg aagctcccgc cgccgagctt cttctttccc
    45661 ttgctcgccg cgccgggtgt gggcgtcggc tgcgtgtccg ccgccgtcgc gagccgggcc
    45721 ggggcgccga gaaggagcgc gagaagcgcg agcgatgcgg tgaaggagcg tttcatggct
    45781 gcgtcgtccc ttctcgcccg taccggtcct cgaggcggac gacatcgtcg agttccggac
    45841 tggagacctc gacgacgagg acgtcctccc cgtcggcgaa catccggtgc ttcgtcccgg
    45901 gcgtcacatg gaaggtctct ccgggagcga gggccaggag atacctctct ccattcgact
    45961 ccgtctccat accgagagtt cccgaaagga cgtggatcgt ctcttccttt gtccgatggt
    46021 actggtagga aagggtttgt ccggcgagga tcgagatcag ctttccggcg tatcggccgt
    46081 tggccgcgaa gatccgttcg cttccccagg gctttttcac gattcgatcg gtcgtcttca
    46141 tacgttcacc gcagcccgcg tttccgggtc gccgctcagg agcgcttcgg cctcccgcgc
    46201 cagaagcgcg gacacgtcgt cgggcgtcga aagggaaagc gcggcgcgtg ccatccgcgc
    46261 ggcatcggcc gcggaggcgc agcgcgcaat gtccttgagg agggggacgg atcgcggccc
    46321 catcgagaac tcccgaatcc caagtcccag gaggaggagg aagagggccg ggtcggcggc
    46381 catctcgccg cagatggcga gcggcttgcc ggcagcacga gcggcgtcct ggaccctcgc
    46441 gatgagacgg aggatcgccg ggtgctgagg ccggtagatc tcggacaccg cctcgttggc
    46501 gcggtcggcg gcgagcgtgt actggatcag gtcgttcgtc ccgagcgaga ggaagtcgac
    46561 ctcgggcgcg atcaggtccg cggtgacggc ggccgacggg acctcgatca tggcgccgat
    46621 cgggacgctg tccggaacgt cgatcccgcg ctcgacgagc tcctggcgcg cttcctcgac
    46681 gaaggcccgg accctccgga cttcctcgac cccggcgacc atcggcacga ggatgcggag
    46741 gtcgcctccc gacgcgaccg acagcagcgc ccgcagctgg gtgcggaaca tctccgggtg
    46801 actgaagcag agccgcaccc cgcggaggcc gaggacggga ttctcctcgg cgccggacag
    46861 gtgccgggct cccttcttcc cgccgaggtc gtaggtccgg atcacgacgg gccggggggc
    46921 gagacgggag acgacctgcc ggtagatcgc ggcctgggtc gcctcgtcgg ggaactcgac
    46981 gccggccgag gagaggtaga ggaactcgga ccggaagagc ccgacgccgt cggctccgta
    47041 ctcgaagacg tcggggacct cgcgagcgag ctcgatgttc gcccggatcg cgacctcgac
    47101 cccgtcccgg gtcgtcgcgg ttcccgacag gctccgctcc ttcagcgaga gctcccgggt
    47161 cgcgtcgcgt ccctgacgtt cgcggaagag cgtgaggacg tcctccgagg gctcgcgcca
    47221 caggacgccc tcccggccgt cgacgatcac gcggtcccct tctccgacgg acgcgaggag
    47281 cttcggaacg gcgacgacgg ccggcagacc gaaggagcgc gcgaggatcg cggcgtggga
    47341 ggtcttcccc ccgcgctcgg tcacgaaggc gacgaccttg tcgcggggga tccggacggc
    47401 gtccgacggc gtcagctcgt cggcgacgag gacgcaggag tcccagggga gggtcgccag
    47461 tcggtcgacg gcgccgcttc cctcgccgag gccccgcttc aggatcgtag cgatgtcgtc
    47521 gaggtcggac gcccgaagcg cgaggtcctt gtcgggaagg tcccgaagcc gcgctgccag
    47581 cgcgtccgcc acgacctcga cggcccactc cgcgttcacc ttctcgagcc ggaccttctt
    47641 ctcgatcggc ccgaggaagg acgggtcccg gaggaagagc gcgtgcgcct ggaagatcgc
    47701 ggcgtactcc ttcccgaggc gcgtctcgac ctgtcccgcc atgtcgccga tctcgcggat
    47761 cgccgactcc acggcgagcc ggagccgctc gagctcggcc ggcacctcgt cggcgtcgag
    47821 gtcccgccga ggcgtggagt cggaccggcc gatgcagacg acggcccggc cgacggcgat
    47881 gccgggagac accgggattc cggtgagcga gagggaccgc ttcatgcccc tcgattgtag
    47941 cctcgcgccc gaggctactc ggactcgtcg aagcgccggt cgacgaggtc cgtgagggcg
    48001 cgcatcgcct cggcctcgtc gtcgccgtcc gtccggacca cgatcggcgt ccccttcccc
    48061 gcggcgagga ggaggaggcc gaggatgctc ttgccgtcga cgtcctcgcc gtccttcgag
    48121 agcgtgatcc gcgacgagta gtgcgcggcg gtgtggacca gcttcgcggc cgcgcgggcg
    48181 tggagtccca gccggttctt gaggacgacc gtcttctcga tcacgcggtc cgcttctccg
    48241 tctccatcag ctcgcccgcg gcgacgatcg agcgacgtcc cttctcgacg agcgtccgcg
    48301 ccatctcgcc cggctccttc ccctctcgcg cgagggcgcg cgccttcacg accatcggga
    48361 ggttcacgcc ggtcacgatc tcgatccgcc ccttctggag gaaggcgagc gccaggttcg
    48421 agggcgtccc gccgaacatg tcggtcagga ggacgacgcc gcgcccgcgg tcggcatcgg
    48481 cgatcgcccg ctggaggcgt tcgctcgcgt gctccggcgc ttcgtcccag gcgagcgaca
    48541 gcgccccgag aggctcgccg ggctgcggct ccaggacctg caccgtctcg cgcagcgcgt
    48601 cggccagcgg gccgtgggag aggaggagga tgccgacgga gcctgcggcg tcggcgacgg
    48661 gagcgtcggg gttcttcatg gtccttcccg gttgtcgtcg cggtgcgaga cgcggacggg
    48721 gtaccccgcc gcggcgaggt cgcggccgag ccgctcggcg acgtagacgg agcggtggcg
    48781 tcctcccgtg cacccgacgc cgatcgtcag gtaggcgcgg ttctcacgcc ggtagttcgg
    48841 gaggcagaag aggaggaagt cgaggagccg gccgtagaac gggcccgtgg cgctcccctc
    48901 ctcgatgaac cgggcgacgt tcgggtcgcg gcccgtccgg tggcggagct cggggacgaa
    48961 atgggggttc gcgaggaacc tcacgtcgaa gcagaggtcg atcgagggcg ggaccccgtg
    49021 cttgaacccg aacgagagga tcgagaccgc gaggaccccc gggtccccgg gcgaccggaa
    49081 gtgctcggcg aggaccgacc ggagctcgtg gacggtcatc gcggtcgtgt cgaggacgag
    49141 atcggccttc cccttcacct cggcgaggag actccgctcc gtcgccatcg cttcggcgag
    49201 cgtgcggtcc acggcgaggg gatgcggtcg acgagtctcc gagaagcgcc ggaagatggt
    49261 cggctcgtcc gcctcgagga agacgaccgt cacaggaagc accggtcgca gggagtcgag
    49321 gaggggcggg aacgcctcgg caaagccggg gttcctcacg tcgaggacga ccgcgttgcg
    49381 gccgggcccg cccttcccac gcgcggcctc ctccacgaag ctccggagga gcgccagcgg
    49441 gaggttgtcg atcgtgttgt agccgatgtc ctcgagggct cgcgccgcgt acgacttccc
    49501 cgatccggag agtccggtca cgacgaggag ccgctggtcg tcccgcgggc ccgtcacgcg
    49561 ccacccccgg tgcgaggccg ccggcggccc cgtcggcgct ccctgtcggt cgaaccggtc
    49621 cggcgggcga tctcctcgtg gacccggtcc gccatctcgc gcgccgcgtt gtatcccctc
    49681 ctcttcagga ggtggttgcg tgcggcgatc tcgacgagca gcgcgacgtc ccggccggtc
    49741 gcgaccggca tccggaccag ggggaccggg acgcccagga tcgcctcgga ttcctcgtcc
    49801 aggccgagcc ggtcgtactc gacgccctcg acccagtgcg agaggcggat cacgagctcc
    49861 accgtgtgct tctcgaggac cgcggcgacg ccgaacagga gcgggacgtt cacgatgccg
    49921 aggccgcgca gctccatgtg gtgacggatg acgccggccg aggagccgac gagcgcgtcg
    49981 ttgtggaacc gcttcacgac gacgaggtcg tcggcgacga gccggtggcc gcgctggacg
    50041 agggcgaggg cgcactcgct cttgccgacc cccgagtcgc cgaggaggag cgtcccgagc
    50101 ccgcctacct ccacgaggac gccgtggagc gtcacgcgcg gcgcgagcga ctcctcgaga
    50161 aaggccgtga gcccttcgat gacggtgctc gtcaagcgcg gcgtcgcgaa gagcgggacg
    50221 tcgcgctgct gacagaagcg tcgaaggacg gccggcggac ggatcccctt cgtgacgatg
    50281 acgcacgaca ttccgagcga ggtgaacgcg tcgaaacgct ccttcaccac ccgggaggag
    50341 agcgtccgca ggtagtcgaa ctcgctctcc ccgaggatct ggacccgctg cgccctcacg
    50401 tacggcagga acccggcgat cgctaggccg ggcttctgga cccggggcca ctcgatcggc
    50461 cggtccagac cggcccgccc ggagacgagc ttgagcccga gcggcgccag ggcgggcgac
    50521 tcgaggtcgg ccgcgcggac gtgcggcttc gtctccggag gggtcgggag catcgagcgc
    50581 ctaacgcggc gtcaggatga cgaccgtgcc gtccttgcgg cggtagagga cgtggagctg
    50641 ctcgtcggac gggtcccggt agacgaggac ctcgcgcttg ctgcggtcga aggcgtgaag
    50701 ggcgtcctcg tcgaacatcc ggcgggcttc gtagctctcg gtccgcggcg ccttctcggg
    50761 gggcgcggtg gccttctcga gaggcagcga gacctcgggg ctccggacgc cggaggtcgt
    50821 gtgcttcttc tcgtccttga cccgggcctt cgtcttcttc gcctgcgcct cgaccctctc
    50881 gagcgcgctc tgcgcggccg tcagctggtc ggcggcgacc gcctcggccg cccacgtccg
    50941 ctggcgcgcc gtgatcgtca cctcgacgag caccccgcgc ttctcgaggc ggacgacgac
    51001 cttcgcctcc gcctcccgcg ggaggacctt cgagagcttc tcgaggcgct tggcgatcgt
    51061 gtccttcgtc ttcgcgtcga cggtgagacg ccgggcggtg acttcgattt tcatcgtgcc
    51121 tcctcgatgg tcatcaggcg tcgggcctca gtccggcgat ccggccggcc cgcgctccac
    51181 tttccgtatc gagctggagg gaatgtggag ctcctcgcgg tacttcgcca ccgtgcggcg
    51241 ggcgattcgg atcccctcgc gcgcgaggag ctcggagagg cgcgcgtcgg agaggggctg
    51301 cgacgcgtcc tcggcgtcga cgagggcctt gatcttcccc ttcacggcga gcgaggagac
    51361 gtcctcgccg gccgaggagc tgattgccga gtggaagaag aacttcatcg gcaggaggcc
    51421 cttcggggtc gccatccact tgttcgagac gacccgggag acggtcgact cgtgcatgcc
    51481 gatgtcgtcg gcgacgtcgc ggaggatgag cggcttgagg tgcgcgacgc cccactcgag
    51541 gtagtcgcgc tgcttccgaa cgatcgactc ggcgacgcgg acgatcgtcc gcttgcgctg
    51601 gtcgaggctc ttcatcagcc actgggccgc ccgcatcttc tcgcggaggt agctccgccc
    51661 ctcgccgtcg agcggtccgt tgctcgagga gaggaggcga cggtagaacg gcgacacgcg
    51721 aagccggggg aggccgtcgt cgttgaagag gacgacgtac tcgtcgtcga ccttcacgac
    51781 ctggacgtcg ggctccacgt agatcgtccg cgtggagtcg tagcgccggc cgggcttggg
    51841 gtcgaggcgc ttgaggacgt cgatcgcctg ctgcagctcc tcggccggca cgctcatctg
    51901 gcgcgcgagg accgggaccg ccttcgtcgc gaggtcgtgg agccggttcg tgacgagctc
    51961 ctcgaggagg gggctttcga ccccttccac gcgcgcctgg atcaggaggc actcggcgag
    52021 gcttcgggcg ccgactccgg gcgggtcgaa cgactgaaga agctcgaggg cctcagccgc
    52081 atccgcttcc gtgcacggga tcgcggcggc gacctcctcg agcgtcaggc ggaggtagcc
    52141 gtccgcgtcc aggttcccga tgaggtactc gcacgcggcc cggacggcgg gggccgcctc
    52201 ggagaccccg agctgctcgg cgaggtgggc gtcgagcccc ggcggggcct ccgcccggtt
    52261 ctcgagcgag tactcctccc cttcctcgtc catgcggggc gcggtggacg agctcccctc
    52321 gaggtagtcg ccgaagtagg cctcgaggtc gatcgcctcg aacgactcct gggtcggctc
    52381 cgccgggatc tcggcttccg ccgtcccgac ggccgggggc ggcggggcga cctcgccggc
    52441 ggctcctccc gcgtcgtccc aggccagggc cgccgcgtct cccgcgtcgg tcccctccgc
    52501 ggggcgctcg cgcgggtcgg ccgcgtcggc cgcctcggcg ctctccctcg ccgccacggc
    52561 ctcgtcgacc tcggctccgg ccgggacctc cgcggcctcg tcctcctcca ggaccgggtt
    52621 ctccacgatc tcctggttga cgacgtcgtg cagctcgagc cgggtcatct gcagcagctt
    52681 gatggcctgc tgcagcgtcg gcgtcatgac gagccgctgc gagagcttga gggagagctt
    52741 ctgttcgagt cccacggtgt gagccggccg cgccggacgg cgcgcttcct acttcgattc
    52801 tagtccagcc ggaagcggtc cccgaggtag atgcgccgca cctcggggtc ttccgaaagc
    52861 gtcaggggcg agccggtccg gaagatcctg ccggcgttga tgatgtagcc gcggtcgacg
    52921 atcgcgagcg tgtcccggac gttgtggtcg gtgatgagga tgccgattcc ggtcgtggcg
    52981 agctcgcgga tgacgtgctg caggtcgagg acggtgatcg ggtcgatacc ggcgaacggc
    53041 tcgtccagga ggaggaaccg cggcgagagc gtcagggccc gcgcgatctc gaggcgccgc
    53101 cgctctccgc cggaaagcgt atcggcgacc gaggccgcga ggtgtgagag cttgaaccgg
    53161 tcgagcagct cgtgcgcgcg ctcgagccgc tcgttgcgcg agagcggcag cgtctcgagg
    53221 atcgccacca ggttctggag cgcgttcatc ttccggaaga cggacgcctc ctgtgggagg
    53281 tagccgacgc ccagccgcgc cctccgaaac atcggcaggg cggtgatctc ttcctcgtcg
    53341 aggaggacgt gcccgtcgtc aggctcgacg aggccgacga cgatcgagaa cgtcgtcgtc
    53401 ttccccgccc cgttcgggcc gaggaggccg acgacctcgc ctccgttcac ctcgatggag
    53461 acgccgtcga cgacggtgcg tccccggtag ctcttcttca gcgcctccgc ggcgaggcgt
    53521 ggtcgtggct cgctcacgtt ccctccggcc ggaggacccc ctggctgggg atgcccgggc
    53581 ccgtctccac gtcgacgcgg ccgcttccgc gctggaagcg cagccgcgcg ccggtcattc
    53641 gattcccctt tccgtcgagc gccgtcgccg gccggccctc gagggcgacg gcgtcgacgc
    53701 gagggtccca ggtcgccttc gccccttctc cgcggcggcc gtcggcccgg tcctcgacca
    53761 cgacgttccc gcgggattcc gcgtggtcga tcgtccgctc gggaccgagg acgacgtcgg
    53821 tcacgtcggc cttcatcgtc caggtcccgg agacgacgct cgccgaccct tcgatccgga
    53881 ggagccggtc cgattcgcgg tgcgtcagga cgctccccga ggccgtgacc gtctcgaccg
    53941 tcgccgcctt cgtcttcggg tcgacgcgac gtctccggag gacggctcgc acgtctccct
    54001 ccgcgcggag cgaacggtcc cggtcgtcga ggaggatcga atgtccgcgg aggacgttct
    54061 cctcctgcca ggccgtgacg ttccccgtca ggaggaccgt cttctcatcg gagcgcacgc
    54121 gaagaaagtc cgactcggag aagaccggcg cgtccgagcc gcctcccagg acctccgagc
    54181 cttcccggcc gcggaacgtg gtcttcacgc cgccggcgcc ttcgatcctc tgctcgcgac
    54241 ggaaccaggt gaggacgtcc gcggcgacgc gagcggtctc gtcctcgtaa cgggccttct
    54301 ccgagccgct tcccgcgaag acggccgact cgtccgcgga ccggagagtc gcggacggag
    54361 cggtcacgcg cgtccgctcg ccttcgctca gcacgacgcc tccttcgagg accgccgtct
    54421 cgagggcccg gccgtcggag ccgaaggcga gctgtccccg acccgcctcg agacggcggg
    54481 acgcgggaac ccgcgcggag cccgcctggg ccggcggagc ggactcctcg agccggaacg
    54541 aggacggaac ctcgacgcgg gcgagccttc cgtccgagag cttcgcctcg aagctctcgg
    54601 cgaccgcctg ccgggcgccc gatcccgcgg cgggtggaag gtcgagccgg gctggcgcgc
    54661 ccgtgaggcc gagccgcgac ggctcggcct cggcgtcgcg ttccagctcg aggctctcac
    54721 cggcggcccg gatcggggcg tcgagctccc cttccgcgcc gcgggcgagg agcgccacga
    54781 cgtcgccccg ggccgtcacg cggtcgccgt ctcccgccag gcgacgctcc acctcgcgcc
    54841 ctcgcaggac gaggtcgtcc gtcttcagga ccacgttccc tcgagcggcg aggccgcccc
    54901 cccgctcgag gtcgactgcg tccgccgtca gcgcgatccg ccggccgcgg tcgtcctttc
    54961 cgccgagccg gacgtcgtcg cggaaacgga cgatcccgac gcccgattcg accgctccgc
    55021 cgagagcccg ggccacgagg gcgccccgca tcgccgtgac cggtccttca gagacgagga
    55081 gccgcgtcag cgggtcgtac cggaaggagc gtcccgcgag gtcgacaccc gctccctcga
    55141 cccgcactcc gtcgaagacg cggaaggcct tcgtggacgg gacgaactcc gcccggggcg
    55201 cctcgaggag cgccgtccgg cccgactcgg cgtcgcgcgt cacgaacgcc acgtccttca
    55261 gccggaaggt ccggtccgca ccgtcggcga acgcgatcgc ctcggcggcc cgaaggcggt
    55321 agacctccga gtctccctcc gtctccgagt agtcgaactc ctcgaaccgc acgcggtcgc
    55381 ggaccccgcc cgactccgcg agaagcgtct cggccacctc ggccggcgtc gtgtccgtac
    55441 gggctccggc cggtcgccac gaaaggacga tcgcgaccgg gaagaccacg aaggcgaggg
    55501 accccaacgc ctggcggagc ctgcggcgcg cgcccggcct cacgccgcct ccgcggggac
    55561 cgcgtcgaga acggtgagcg ccggccggtc caggaacgaa tcgtgagcga cgttcgcgag
    55621 gacgtcccag gtccccgctc tggagcccgt ggagatgctc ccgagcgtcc acccgacagc
    55681 gtccagacgc accccttcgc cctcgaacgc gagccggaga ccgcgctcgc cgaccgggcg
    55741 gccgcgtccg ttccacgaga gggagcgcaa gaggaagagc ggcctcgggt tccccatccc
    55801 gtgaggctcg aggcgtccga ggtgctccac gagctccggc gtcacttccg aggcccggag
    55861 cggtgcgtcg gcctcgagga cgacggacca ttcctcttcg tcgcggctgc gggcgaaggt
    55921 ctccttcgcg tcgtcccgga aggcctcgaa cgtgttcgcg gccaccgaga tcccgatcgc
    55981 cgcgtgatgt cctccgaact ccgcctcgtg ccgccgggcg acgggcgcga tccgctcgtg
    56041 gagcggagtc cgtccccagg tccggccgct cccgccgacc cgcccgtcct cgcgggagag
    56101 gagcagcacg gggcgcgaga cggtctgggc gaccttcgag gcggcgatgc cgaggacccc
    56161 gcggtgccac ccgtcctccg gcgaaccggc ctcgacgacg agagcgtcgc gtacggggtc
    56221 gaacgtcttt tcgagcctct cgagggcggc ggccacgacc cgctcctgca tccgccgccg
    56281 ctcggtgttc gccccctcga tctcgtcggc cagctgcccg gcccggacgg cgtcggccgt
    56341 cgtcagcagc tcgagcgcgc ggaacgcgtg gtcgatccgg ccggaggcat tcaggcgcgg
    56401 agcgatccgg aacgccacgt cgtggacgga cgggctcttg cccgcccgga ccgacgagcg
    56461 cgacaggagg gcggtcaggc ccgcggagcg cggcccggcg aggcccgcga gcccccacga
    56521 gacgaggatc cggttctcgc ccgtgagcgg aaccatgtcg gccaccgtcg agagcgccgc
    56581 gaccttcgcg agcgaggcca tccaggagcg gaccgccttc ccgtccagcc cgacgcgggc
    56641 gccgtccgcc gtgagaagag cctcggagag cttccaggcg atccccgcac ccgcgagctc
    56701 cttgaacggg tacgagcagc ccggaagccg cggattcagg aggacggcgc cggtcggaag
    56761 ctcttccggc ggcaggtggt ggtccgtgac gatgacgaac gcgccgcgcg ccaccgcctc
    56821 ccggatcggc tcgaccgccg tgatgccgca gtcgaccgtg acgattcctt ccggcgcgtg
    56881 ctcgtcgagg acccggcgca gcgtctcgga gcggaggccg tagccgtccc gaagccggtg
    56941 cgggaggaac ggtaccgcgt cggccccgag ccgcttgagc gtcgtcgtca ggatcgcgag
    57001 cgcgccgatc ccgtcgcagt cgtagtcgcc gaagacgacg accctcgcgc cgcgccggca
    57061 ggcgtcggcg agccgggcga cggccgcgtc catgcctccc atgccgaacg gatcgtggag
    57121 cgacgccggg tccggctgga gccacgctcg cgcctcggcg ggcgaggaga atccccgcgc
    57181 ggcgaggacc cgcgcgagcg gcgcggcgac ccccagctcc cgcacgagga gagcggccgc
    57241 gcccgcgtcg tgaggccgga gtctgcgtgt gacgcgtggc ggcgggaagg ccgaggtcac
    57301 cgggacgccg cgggcccctc ccaggccccc atgcggcgga acttcgcgta gcgggcctcg
    57361 aggagggcgt cgaccgggat cgccgcgagg cggtcgaggt ggcgcacgag cgcctgcccg
    57421 acggcgtcga tcgtcgccac cgggtcggag tgggcgccgc ccggaggctc cgggatcacc
    57481 tcgtccgcga tcccgagggc cttgcagtcg gcggcggtca tccgcatcgc ttccgcggcc
    57541 tcgcgcgcct tcgcctggtc cttccagagg atggcggcac acccttccgg cgagatgacg
    57601 gagtagaccg agtgctcgag catcaggatc gcgtccgtca cgccgattcc gagggcgccg
    57661 cccgatccgc cttcgcccgt cacggtcgcg atcaggggca cccggaggcg cgccatctcg
    57721 aggaggttca cggcgatcgc ttccgagacg ccccgctcct cgctctcgac ccccggatag
    57781 gcgcccggcg tgtcgatgaa cgtcaggacg ggccgctgga acttctcggc gagcttcatc
    57841 acgcgaagcg ccttgcggta gccctccgga cggggcatgc cgaagttccg gcggaccttc
    57901 tccttcgtgt cgcgcccctt ctggtggccg acgatggcga ccgtccggtc gccgaggacc
    57961 ccgaagccgg ccacgatcgc ggggtcgtcc gcgaaggccc gatcgccgcg cagctcgacg
    58021 aagtcgcgca ggaggacgcg gacgtagtcg agcgtgaacg ggcgggccgg gtggcgcgcg
    58081 acgagcgtct tctgccaggg agtgaggttc gcgtagatct cccccgagag cttcacgagc
    58141 ctgtcggaaa tcttgcggat cgccttgtcc cgggcggggc tcccggggct ctcccgcagg
    58201 ccgtcgatgc gcgctcggag ccgctccagc ggctcctcga accggtgatc ggaactcacc
    58261 cccggaatgt accagaccgc gacggccggg ccgtcagagg agactctgtg ggtcccggtc
    58321 cagccggggc ggttccggca gggaccggag cagttcgccg gccgccgcga gcgcttcgcg
    58381 ggagtcggac cggacgagca gctggacgcg gtagagcccc ttgagccgct ccagcggcgc
    58441 cggcgccgga ccgagcaggc ggagcttcgg cagcccagcg atcgcgaccc ggccggcgcg
    58501 cgcggcggct tcggcttcgg ggagctccct cgccgtccag agcgcgagga ggaggtggga
    58561 aaagggcggg tagccgaatg cccggcggaa ccggagctcc ccttccgcga aggaggcgtg
    58621 gtcctgggcg acggcggccg cgatcgcttc gtgctctggc ctggccgtct ggacggccac
    58681 gatcccgggc cgctcccccc ggccgacccg gcccgccgcc tgggtgacga gctggaacgt
    58741 ccgctccgcg gagcgaaagt cggggaacga gaggagcgcg tcggcgtcga ggacggcgag
    58801 gaccgtggcg ttcgggaaat cgtgaccctt cgccaccatc tgcgtcccga ggagcgcctg
    58861 cgcggcgccc gactcgaagt cggcgaggac gcggaccgct ccgccgcgcc gcgcggccgc
    58921 gtcccggtcg aggacggcgt acgggacctc cgggaagagc tcctcgaacc tctcggcgag
    58981 gcgctcggtg ccgaagccga cgggcatgag gacttcggag ccgcacgagg ggcaggctcg
    59041 gggcctgggg acgaagtcgc cgcagtagtg gcagaggagc ctctccccgc gccggtgata
    59101 ggtccgggcg accgagcacg aggcacagcg gaagtcgtgg ccgcaggagc gacagaggag
    59161 ggccggcgag aagcctcggc gattcaggag gacgatcgcc tgctcgccgc gctcgaacgc
    59221 cccgcgcagg agctccaccg tccgggcggc gaaaaggacg cggccatggt cgccggggcg
    59281 ggcggcctcg ccccggaggt cgacgacctc cacgcgggcc ctctcgcgcg agccggggcg
    59341 cgccggcagg acgaggcgcg gcaggcgtcc gtcgcgcgcg gcctgctcgt cctccatgga
    59401 cggcgtcgcc gtgccgagga ggaggaccgc cccctcgccg tgcgcccgca cccgagccac
    59461 ggtccgcgag tcgtaccgcg gcgcctcggc ctgcttgtag gagctctcgt gcgcctcgtc
    59521 gacgacgatc agcccgagcc tcggcagcgg agcgaagacg gcggagcggg ggccgaccac
    59581 ggcgtcgacc tccccgcgtc gcccccgctc ccaggcggcg gagcgctcac cgtccgagag
    59641 gccgctgtgg aggagggcga cgcgcgagcc gaaccgggcg acgacccgcc ggatgagccc
    59701 gggcgcgagg gcgatctcgg gcaccaggag gatcccctgc cggcccagcg cgatcgccct
    59761 ctcgagcgcg gcgaggtaga cctccgtctt cccgctgccg gtgacgccgt cgaggaggaa
    59821 tcgcgcctcg ccgccgcgct cgagcgcttc ggcgacggcg tcgatggcgg ctcgctgggc
    59881 cgccgtcggc tcgatgggag ccgtggcgcg cgccggcagc gcgtgcgcgg ccgcgtccgc
    59941 cggcctttcc tcctcgacga cggcgacgag agactgtttc gccagcgcgg ccagcgtgcc
    60001 ggccgacacg cccgcggccc ggagctccgc ccccgacgcc ggtctcccga gcgccgcgag
    60061 cgtggcgagg gccttccgct gcccgggccg cctcgcgagg cgggggtcgt cggcgctccc
    60121 tcgcgccgcg aagctccggg tcgtgggagg ccgcgcggcc cgaagctcct ccgagggaat
    60181 ccggacgagt cccgcgtcga ggagcaggcg aatcgccttg ccgaggcccc gggagccgag
    60241 cctctcaccc agctcgagcg ccgtcgcgcg gcccgacgtc aggacctccg tcaggacggc
    60301 ccccgtgagc ccttcgggcg gatgcgctcc gaccgcgcgg ctcgtcggga cgtaggccgc
    60361 agtcccggcc gcgagaaggc gagcgggtac ggccgcgcgc aggagctctc cgggcggggc
    60421 gaagtagtaa tcggcggctc gcgtcaggag accgacgaac gactcggaaa ggaagggctc
    60481 ctcgtcgagg accgtgagga cctcgcgaag aacggtcccc tcgggagccg ggtccggctc
    60541 cccggggacg acgaggccgg taaggagccg cggtccgaag gggacggcga cgcgcgcgcc
    60601 cgccgggggg agaggctgct cggacggaat gaggtacgtg gcgagccccg gaaacccggt
    60661 cggaaggagg accgcgattc ttcgggcgac gtcgtcggac gtcacggtgt caggccgtcg
    60721 tgccggtccg gaggtccggc tcgacccagg gggcctcgtc accctccccg gcctccgcgt
    60781 cgacgtcgac gaagcggccg gcggacggct cccagcgcca ccttcgggag acgtcctgct
    60841 cgacgtcctc cgagacgacg aggaccgaac ggtcgatgct caggtccccg ccggcgagga
    60901 ggaggcctcg gtcgacccag acggcgggcc cgaagccgcc cccctcctcg ccgaagtaca
    60961 cggcgacgtc gaaaaggggc tcgggcccct cccgccttac gaacgcctgc gcgaagtcga
    61021 gccggccgtc gaaatcgaag tcgccccgga cgaagtacgg gcggtaggcc ccgtagaggt
    61081 cccgtagctc ctgccgcgcc tccgattcga gggcgtcttc gtcggtggcc ggccggacgc
    61141 cgctggtcca cgtggccccc acggcctcct cttcggcctc cgttcgcccg ctcgggggcg
    61201 gcccgcggcg tcccttctcg acctcgccgg ggagccgttc ccctcctcgt gcccgggccg
    61261 aaaggacgag tgccgtaccg aaggcggcga aagcggcgag gaacatgacg ccgacgaccc
    61321 ggcgagcgat cacgagccgg actccttccg gccggggacc gggaggccca gcttccggag
    61381 gacctgctgg aggtcctccc acgcttcctt cttccgctcg ggctggcgga ggaggaacga
    61441 ggggtggaac gtcggcatca cggcgatccc tcgacgcatt ccccactttc cgcggaggcg
    61501 agtgatcggc tcttcggtct cgaggaggaa ctgcgcggcg aacttgccca tggcgacgag
    61561 caccgcgggc ttcacgacct cgatctgggt ctccaggtag ggccggcagg ccgcgatctc
    61621 cgccggctcg gggttccggt tccccggcgg acggcacttc aggacgttcg cgatgtagac
    61681 atcggcccgg tccagaccca tcgccccgat catctttgtc agaagctgtc cggcgcgacc
    61741 gacgaacggg acgcccgtcg cgtcctcgtc ggcgccgggg gcctctccga cgaacatgat
    61801 gcggctgcgc ccggtcccgt cggcgaagac ggtttgcgtc cgggtgcggc agagaccgca
    61861 gagctggcat gccgcgacgg tggcccggag cgtgtcgagg tcgggaaggg ccggaacggc
    61921 cgggcccttc gcggaggccg ggcgcgtgga cggcacggcg tctctcgtct ccggccgcgc
    61981 gcccggtcgc gcggacgtcg gccccgcgac cgcgggcgga gccgcgaggt ggagctcgcg
    62041 gacgccgagg tcccggaagt agccgagcgc ggcgctcagc gcgtcgtttc ggcgggggcg
    62101 gcgggcgggc atctctcgtc gaggagcgcc gcaaggcgct cgaggatcgt ggcggaaacg
    62161 accgctttcg cggcgcgagg gacggcgacc ggctcggacg cgccggcggc gagaaggagg
    62221 acctcgttct cttcgctgtc gaagccgatt cccggccgcg agacgtcgtt cgcgacgagg
    62281 aggtcggccc ccttgtcgag gagcttccga cgcgcgttcc cggcgaggtc gtccgtctct
    62341 gcggcgaacg cgacgacgac ctgccgcggc gtcctcacgg cgcggaccgc ggcgaggatg
    62401 tcgggggcga gtgccaggcg gatctcgggg atcccgccgg cccgcttgat cttcttcggc
    62461 gtaaaggcct cggcctggaa gtcggcaggt gcggccgcca tgacgagggc gtcggaggag
    62521 gggagctcgg cgaggacggc ctcgagcatc tcggccgtcc ggtcgacctt caggagcttc
    62581 acgttcgggg gcgggtcgat ggcgaccggg ccgctcacga ggacgacctc cgcgccgcgc
    62641 ttctcggcct cgcgtgcgag cgcgtagccc atccgcccgg agctgcggtt cgtcaggaag
    62701 cgaaccgggt cgatcggctc gcgggtcggg ccggccgtca cgacgacgcg ccgcccctgg
    62761 aggctcctcg agacccgcgc cgcgccgacc acggcctcga cgatccgcgc cggcggggcc
    62821 atccggccgg gaccgacgtc gccgcaggcg aggtcgcccg aatcggggtc gacgacgacg
    62881 gccccgcgac cccgcaggat ccggaggttc tcctgcgtcg ccgggtgggc ccacatccag
    62941 gtgttcatcg ccggcgcgac gacgatcgcc cggcggtgcg cgagcgcgta ggtcgagagc
    63001 gcctcgtcgc cgatgccgcg ggccatcttc gcgaggacgt ccgcggtcgc cggcgcgacg
    63061 gccaggacct ctccccagcg cgccagctcg atgtggtcga cggcgcccga ggcagggtcc
    63121 cagaggtccg ccacgaccgg cgacttcgaa aggaccgaga gcgtcagcgg cgtgatgaag
    63181 cgctgggcgg ccggcgtcat gaggacccgc acgacggcgc ccttctcgac cagcagccgg
    63241 acgatctccg ccgccttgta ggccgcgatg ccgccgctga cgccgagaag gacgcgcggg
    63301 gccgggaagc cgcctccgct cacgccgacg tcagggcgtg atcagcggga gcgggtcgat
    63361 cggctcgatc gcgcccgttt cgagctcgcc ctcgaccgcg ccttcgatgg ggcgctggcc
    63421 gagctgccac ttcacgtggc cgcccgccac ctcgtcgagg gcgattcggg agtacttcgt
    63481 gtgcttcgtc tcgaccttcg gccgggctcc gagcatcagc tgctcggcgc ggcgggaggc
    63541 gatgtggacg agccggaact tcgactcgat ctgcggggac ggcggggtcc catccggggt
    63601 ctcttcgggg gtctcggtca tcgggctggc tccggtctcg tcgttcggtg tcgtcacttt
    63661 caccctcgat tcggccccgt tacgcgggga acgtccgcag aatggcttcg agccgttcct
    63721 gccgccgacg gcggctcgaa cggcgcgcca cgacgatcga ccgcagctcg tctacggccg
    63781 cgtcgaggac gtcgttgatt atcgcatagt cgtattcgcc gaactcggcg agctcccgag
    63841 cggccgcccc gagccggcgc gcgatcgact ccggggtgtt gtgcccccgg gcggtcaggc
    63901 gctcctccag gacctggcgg gacggcggga agacgaagac cttcacgacc tccggcatgc
    63961 gcgaccgcac ctgccgggcc ccctggacgt cgatgtcgag gacgacgtcg atcccggcgg
    64021 cgaggcgggg ctcgacctcg ccgcgcgagg tgccgtagcg gtgcccgtcg tagaccgcgt
    64081 gctcgaggaa cccgtcccgg gccgcgagcg actcgaactc ctccggagag acgaagtggt
    64141 attcccggcc gtgaacctct cccggccgcg ccgggcgggt cgtgtgagag acggagaagt
    64201 gaatcgtccc gacgaacgcg gggtcggcga ggatccgacg gatcagcgtc gtctttccgg
    64261 cgccggacgg ggacgagacg atgaagaggt ctcctgcggg tctcatcccc agagtatgcg
    64321 aggtacccga ccgcccgtca gcaggaacgc gctcggggag tccggggggc ggcgaacagc
    64381 gccctttggc aacgtcactc cacgttctga atctgttcct tgagcgcctc gacatccgac
    64441 ttgaggtcga ggaggacgcg cgtgagcggg agctcgcggg ccttctggct cgccgtattc
    64501 gcttctctgt ggagctcctg ggcgaggacg tccaggcgct tcccggacgc atgctctccc
    64561 gacatcagcc gtcgggcttc gacgagatgc gcccggaacc ggtcgacctc ctccgtgatg
    64621 tcggcccggt ccgccaggag cgcgatctcc tgggcgagcc gggcctcgtc cagcgggacc
    64681 cccgaggcga gcttcgcgac gcgctcgcgc agctgcgcga ggaggcgctc ggagagcccc
    64741 tcgcgctcgt tcgccacccg gccgatcccg tcctcgaggc ggtcgaggat cgccgcgagg
    64801 gcggaacgga ccgcctcccc ctcgcgcctg cgagaggcgt cgaggtcgac gagggcggcg
    64861 cccgcggtct ccaggatcgt ggcggcgaga gtctccccgg cctcgtcctc ggcgatgcgg
    64921 acgacccccg ggagagaaag gaggtcgcgg gccgtcagct cccgcggcag gtccctctcc
    64981 cgcgcgaggt cgccccaggc ctgcgcgtaa cgctccgccg tcgcaccgtc gatcgtcacg
    65041 gctcccccgg cgggacggct cacccggacg gagaggtcga cgtgcccgcg ggcgacggtc
    65101 gcgccgacga gcttgcgcag gggggcctcc aacgcgccga ggtcgtcccg catcttcagc
    65161 gagaggtcga ggaagcggtg gttcacgctc cgcagcgtga tcgagagctg cgtcccgtcg
    65221 gggagcgcgg cctgggcgcg gccgaagccg gtcatcgact tcacggtgtc ttctcctcct
    65281 cgaaggccgc gaggtcgtgc agcctccggt agacgccgcc gagcgcgagg agctcggcgt
    65341 gccggcccct ctcgacgagg cggccgcggt ccaggacgac gatctggtcg gcccgtcgca
    65401 ccgtcgagag ccgatgggcg atgacgagcg tcgtccgccc cgccatgagc cgctcgagag
    65461 cggcctgcac ggcccgctcg ctctcggtgt cgagcgcgga tgtggcttcg tcgaggatca
    65521 ggatcggcgg gtccttcagg agcgcccgcg cgatcgcgag ccgctgccgc tggcctccgg
    65581 agagccggcc tcccgcctcc ccgaccggcg tgtcgagccc cttcgggagg gcgtcgacga
    65641 agtcggcggc gttcgcggcg gcgagggccg cgcggacgcg gctctccggc acgtccgcga
    65701 ggccgtacgc gacgttcctc cggacggtgt cgtcgaagag gaccacctcc tgagtcacga
    65761 gcgccatcgc gccgcggagc gaggagagcg tcacgtcacg gacgtcgacg ccgtcgatcg
    65821 agatccgccc cgcggtcacg tccatgaagc gcgggaggag gttcgcgagc gtcgtcttcc
    65881 ccgctcccga cggaccgacg agtgccgtca ccgagccacg cgggacgacg aggtcgacac
    65941 ccgagaggac gtccggcccg cccgggtagg cgaacgagac cccctcgaag cgaacctccc
    66001 tctcgaacgg ccggagcggc gcggcctcgg gccggtcgac gacgaggacg ggggcgtcga
    66061 ggacctcgaa gagccggcgc gcggacgcga gcgcctgctg gagggcgagg ttgatccgcg
    66121 tcgcccgctt gaacggctgg tacatcatca ccagcccgac cccgaacgag atgaacgccc
    66181 caagcgtcat cgtccccgct tcgatccgct tccccgcgta gccgaggagg accaggaacg
    66241 cgaggaccga cgccgtctcg acgagcggcg aggaaagggc catcacgccc gcgcccttcc
    66301 gaaggaggcg gaacgtcctc tcgttcacct cgccgaagcg gtccgcctcg tacttctccg
    66361 ccccgtaggc ctggacgacc cggtggccgc gaagcgtctc ggagaggacg ccggtcaggt
    66421 ctcccatccg ctcccgcccc gccttggaga tccgccggag ccggttcgcg atcaggacga
    66481 ccgggacgac gatcgccggc gcgacgacga gggcgacgag cgtcagctgg ggagagcgcg
    66541 acacgacgag gaccgagagg ccgaggagcg tcgcgattga ctgcacgagg tcggcgaggt
    66601 cggtcccgaa caggctctgg atccggtcga cgtccccggt cacgcgcgag atcaggtccc
    66661 ccgaggggtt cttcgcgtga aaggcccccg actgaccgag gagccgctcg tagacgaggc
    66721 gccggacgtc cctcacgaac gccagcccga ccgcgttgaa ccggtattcg gcggcgaacg
    66781 cgcagaggtt cttcaggacg aatgcgacga ggaggatgac cggcaggacg acgccccggt
    66841 tggcctcggt cacgccgacg gcggactgga ggtcgagcag gcgatcgtcg agccactgga
    66901 tgacgggcgt cttccgggcc gactcggcgg cgccgagcgc cttgccgagg ccgcgcgcgg
    66961 ccggatcggc ggccagctcc tgcttcacgg ccgagctctg ccccggggtc aggacctggt
    67021 cgaagagcgg cccgaagagg taggcgacga gagtcgtgaa gacggccacg ccgaccatcg
    67081 cgagaacggc gacgagcgcc gcccgcgcgt gcgggcggaa gaggcgcgcg aggcggagga
    67141 agtccctcac gcctccctcc gaccgaggag ggcgaggacg gcctcggccg tccgctccgg
    67201 agcgcccggc tctccgagct cctcccgccc ctcggcgagg cggcgccggg cctccgcggc
    67261 gtgcgaggcg tcgtcgagga agcgctcgac ctcggccgcc acccgctcgg gggtcgccgc
    67321 gccctggatc agctccggca ccgcccgcgt gccgtcgccc gggtcggaga ggatgttcgc
    67381 catcgccacg ctcggcacct tcacgaggag cttgcccagg aggtagctga tcgtggcgac
    67441 cttgtagacg acgaccatcg ggatcccggc gagaagcccc tcgaccgtcg cggtgccgga
    67501 ggccacgagg aggacgtccg acgcggcgag gagcgagagg tgcggcccgg agacgacgct
    67561 ccagcgatcg aggcgctccc ccgcgatgcg ccggaggagg tcgtcgggaa ccgagtcggc
    67621 cttcacgagg acgacctcga ggtcggaccg gcgcacctgg aggagggcga cggcgtcccg
    67681 caggatcgga aggagcgacc ggacctcccc cgcgcgcgag ccgggcatca ggacgacgcg
    67741 ccgccggccg ggcggcggag gggccgccgg agtcgccagc tcgcgccgcg cctcgtcgac
    67801 gagcgggtga cccacccagg tcacgcgctc cccgaggccg cgcgcgtcgt accagcgctt
    67861 ctcgaagcgg aagagcgtca ggacgccgcg gccgaggcgc gcgagcgtcc gcgcccgtcc
    67921 gctccgccag gcccagacct gaggcgagac gtagaagacg accgggactc cggccctcgc
    67981 gagccgtcgg gcgagcggga ggtggaagtc cggagagtcg atcagaacgg cggcgtcgac
    68041 cttcgacgcg accgcctcgc gcgtcagctc gcgcatgagc cggtgagcga agcggatctt
    68101 ccccagcgcc tccacgaccc cgatgatcgc gagctccttc tggtgcgcga tgaggcgcat
    68161 cccgcgggcg gcgcaccggt cgccgccgag gccgaacgcc tcgagcgccg gatcccgctt
    68221 cgagagggcg ccgaggagag ccgccccgtg ctggtcgccg gagacctcgc cggccgagac
    68281 gaggaggcgc acggcctcag cggctcccga cgagcgacat cgcccaggcc gccgcgagcg
    68341 agccgccgac gatcgccgtc cacatgcgcg tgaagaagcg ccgccgggag ggcccgtcgt
    68401 cgcgccagag gagcgcgaaa aaggcggaga ggatcgtcgc gaggaggacg aggtggagga
    68461 actggtcggc gaaaagcgtc gtgctcacgg cttccgttcc cccgcgacgt cccaggcgtc
    68521 gagcgccagg aggacgttca gcacgcccgc gacgacgagg taggtcgtcg ctacctcgaa
    68581 ggtaggagag gagggctccg ccgggccgag cccgaacgcc cggacgagga gcgctggaag
    68641 gacgacggcg tgggacgcgg ccgcggtcag gagggcgccg gttccgcgcg ccggcacggg
    68701 ccccggggat ccgcccagca gaaggcccag gacgaaggcc gcgccgacga ggccgaagaa
    68761 cgccgcgccg cgcgcccttc ggccgcacca ggcgtgaccg agtccgggga cgaggagcgt
    68821 cagcagcgcc gcgacgatgc gcccgctggt cgggcgcggc acgccggtca ggaagccccc
    68881 tccggcgtgc cgagacgcgc gcggagccgg cggaacgtga cctcgaggtc ctcggggacg
    68941 acgcgggtcc cggagacgac cgtcatgaag ttcgtgtctc cttcccagcg cggcacgagg
    69001 tgcatgtgaa ggtggccggg cacgccggcg ccggcgcact tccccaggtt caggcccgcg
    69061 ttcagcccgc ccggcgcgta ctccgcgcga agggcctcct cgatgcgcgc ggagaggtcg
    69121 atcagctcga tcctctcctc tcgcgtcagc tcgggcaggg tcgcgcgatg ggcgtacggc
    69181 gcgacgagga cgtgcccgtt cgagtaggga taccggttca ggaccgcgaa gacgctccgg
    69241 cctctctcga cgatgagggc ctcggcgtcg ccgaggcccg ggaggtcgca gaagaggcat
    69301 cctcccgcgc ccgatggcgg ggcgcccgtc aggtagctca tccgccaggg cgtgaagagg
    69361 acgtcgttca ttcctccggg gctcgggcgc cgggctccga ctcgggcgcg ggggccgtgg
    69421 cgttgatcag ctccttcagc ttctttcccg gcttgaagta ggggacgagc ttcggcggaa
    69481 cgtggacctt gaccccgctg cgcttcgggt tacgcgcgat gcgggcgccg cgctggcgga
    69541 gcttgaagct gccgaaaccg cggagctcga tcttgctgcc ggcccgcagc gaggaaatga
    69601 ttccctggaa gaccgactcg accacggcct cggcctgctt cttcgtcagc tgggccgtgc
    69661 gcgcgacctc ctcgacgaga tccgccttgg tggtcgtctc ctccgcccgg ctgacgccct
    69721 gcgattcgcg ttccatctcc tcccctcgcg gggagcgtcc cggatggacg ccccccgtcg
    69781 gcgcctcctc gcgggcgcct cgaacgtcag tcttccgtct tcgacgtctc ggcggaggcc
    69841 tcggtcgagg ccgcctcgga ctcctcggcg accggctcgg cgggagcggc gacctcgggc
    69901 gccgggtccg atgcagcccg ggcctccggc tccccctcgg cctccggagc ggcgacggcc
    69961 tcggccgcgg cagcgagctc ggccgggtcg ggctgcggaa cgtcccgcat cgagagcccg
    70021 atccgctgct cgtccacctc gatccggagg atccggacgc ggacggggtc accctcggag
    70081 aagacgtccg agagcttctg cccgcgcaag acgtcggtct ccgagacgtg catcaggccc
    70141 tcgaggccga gaggcagtcg gacgaagacg ccgaagtcgg cgaccttggc gacctgcccg
    70201 tcgacgacgt cgccgggctg gtgctcgcgg gagaactcct cccactcgtt cgggcggaat
    70261 tccttgatgg agaggctgat ccggcggttc tcgacgtcga tggcggtgag cacggcctgg
    70321 atctggtcgc ccttcttcag gacctcggac gggtgcttga cgcgccgccc ccacgacatg
    70381 tcggagatgt ggacgaggcc gtcgatcccc tcctcgatct cgacgaacgc gccgaagtcg
    70441 gtcaggttcc ggaccacgcc ctggacgcga tcgccgatcc ggtacttctc ggcgagctgc
    70501 tcccacgggt tcggctccgt cgcgcggagc gagagcgaga ggcggcgggt ctcggggttc
    70561 acgtcggcga tgaccgcctc gacggtgtct cccaccgaga ggagctt
//
LOCUS       DAOONL010000009        69364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_237_length_69364_cov_17.081303, whole genome shotgun sequence.
ACCESSION   DAOONL010000009 DAOONL010000000
VERSION     DAOONL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 69364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69364
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_69364_cov_17.081303"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..558)
                     /gene="rnpB"
                     /locus_tag="PLP50_02630"
     ncRNA           complement(158..558)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLP50_02630"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(597..1199)
                     /locus_tag="PLP50_02635"
     CDS             complement(597..1199)
                     /locus_tag="PLP50_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPA50475.1"
                     /translation="MVEALAHFGTGLGLAAGAGLNAYAVLLVYGGIAHLFPEDFPGAI
                     ARFLAEPATLAVLLALYVLEFFADKIPGLDHVWDIVHTVVRPVAGALLAVAAVAPGAE
                     ASLTVLAGGGGGVIALAAHVVKGTARLTSTALTAGVANFALSLAEDVLAVLQAIVSVF
                     LPLVALVLAVTVGGLFLLCVPKLAKGVNLFTSRRKPRTEA"
     gene            complement(1228..2727)
                     /locus_tag="PLP50_02640"
     CDS             complement(1228..2727)
                     /locus_tag="PLP50_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA50476.1"
                     /translation="MDTPKPGTGSLSGGTLEKTELAEVLRNAYVSRGHAQVLVSHRGE
                     ERSFWFRRGRLVSASSNREAQHVGDLLRTFGLASESVLFAAFERALAEPGRGLASALR
                     EAGAVAPYVADACVRALAEMVLYSTFQWSAGTFSVVPLDSAPDLPVAFDQSTASLVLE
                     GLRRRPPVAGVAARVDPQAKLVLSPDLLLRYQCAVVTSEEAEALDAVDGTKRAADVCL
                     DLRVVERLMSVGLVSVVERKKPPDEHAPTGFQSLNLEVAGTPPSARLAEILEQQTRTV
                     WNAYRRLDWTTAYELLGVAEDAADPDLRRAVHERARLFHPDNALRGTLADAGEALESL
                     FAKVREADRSFASPASRQQYDRLRSSGGLAAPVQYGEPTREVQKQVAKANFQRARTLI
                     EMEDFFPAYEMLRQAVEFDPEKPEYWTLLAKVQARNPKWIRQATETLRRATENLPDSV
                     EVWLAYADALGAERNETGRVKALKEVLKLDPTNRKASKALAEIAATKPR"
     gene            complement(2740..4548)
                     /gene="uvrC"
                     /locus_tag="PLP50_02645"
     CDS             complement(2740..4548)
                     /gene="uvrC"
                     /locus_tag="PLP50_02645"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPA50477.1"
                     /translation="MGRPPDLSVYPDQPGIYLYRDGKGKILYVGKARSIRKRLASYFA
                     AGPKHPKTEALLAEFETIDTVVANTESEALALENAFIKKHKPRYNILLRDDKTYPYVK
                     VTTGEEWPRAHVTRRVLKDGHSYFGPFLPARTCRGAMKMVQRMFQIRTCRIEIDGTLP
                     RPCLYHDMHACLGPCVAGLTTKAAYDDAVRDVVLFLSGRNEELTPRLAAKMEAAAEAE
                     QYEMAAAYRDCLRTVEELAQKQRVQSLAGEDVDVFGDFADGGNVAVSVLHVRGGAVLD
                     SRELFWEGLGEVEPEEFWVALLSQYYDATTFLPREVHLPLEVAEEALEPLEAWLSDRR
                     GTKVAIRTPKRGAAFDRVRIARDNARTSHVRRFRTAREAGEKATLALSKALDLDRRPS
                     RIECFDISHFQGSDTYASCVVFVDGAPAKGEYRAFRIDRPVPDDFASIAEAVTRRYER
                     RRAEGEPLPDLLVIDGGKGQLSAALAALDRVGIELPAVGLAKREEELFVPGRSLPVTL
                     PRRNAGLKLLQRARDEAHRFGLSHHRRARSRRTLQSPLLAVPGLGPASVKRLLAVFGN
                     GEAALAASPEALAAAAGKTAAARIERFRESSGGDRR"
     gene            complement(4587..6233)
                     /locus_tag="PLP50_02650"
     CDS             complement(4587..6233)
                     /locus_tag="PLP50_02650"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPA50478.1"
                     /translation="MRRLSPPAGPIIDGLDVETGRAYVARNPATGALLDEVRRGGPEE
                     IDAAVAAARKAFLHWGARSHQERRKHLEALLEAVRRSHDELARLIATEQGKPVAEARA
                     LDLVPAADALRFLARHADELLAERPVDYEQLLFAHKAASDRYEPLGVIAVITPWNFPF
                     AIPMVEVAACLAAGNTVVLKPASATARTGLAIGELCRMAGLPPGVVNVVPAAGADVDR
                     LLEHPGIAKVLFTGSVETGQHVMRKAAGNVTPIVLELGGKDAAVVAADADLERTANGL
                     VWGAFANAGQACGSIERVYVEREVADGLVSRVVEKTRRLRQGDPLAEATDLGPMTTAE
                     QRDFVDTQVREAVAQGARALTGGERPAAKGNWFPPTVLVDVHHGMRVMREETFGPVLP
                     IAVVDSLDEAIALANDSEFALTASGWTRSTRTARKLVEELRAGTVTINDHLFSFGEPR
                     ASWGGLGKSGIGRSHGVHGLRELVNVKHVSVDLDSSPSAPWWYPYDGAFQQFMRKAFG
                     VLYARDARTRVADGLGLLASGRFFGYVKVSTIASNLGKVL"
     gene            complement(6230..7348)
                     /locus_tag="PLP50_02655"
     CDS             complement(6230..7348)
                     /locus_tag="PLP50_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA50479.1"
                     /translation="MARKKASANPAPSRVVLFTGFPGFIGARLIPRLLELEPDTVFHC
                     LVQEKFLDLARSSVSQLAESHPGTTGRIELVVGDITAPGLGIGETEARLLHRKLTGCY
                     HLAAVYDLAVPREVGMRINVEGTRNVLGFLEECPHLHRFDYVSTAYVSGTAVGLYRET
                     DLDVGQSFKNYYEETKFLAEEAVKGSALPTATYRPAIVVGDSRTGETAKFDGPYFALN
                     AMRQLPSPGAFVKVGTGTNPVNLVPVDFVLEAIARLSTWEGSVGRTYHLTDPRPLSAF
                     EIEELFAKALGKQFVYVPVPTVLAKLLFTPKPIQDWFGMPAVTVEYFDHPARYDSSQA
                     ETDLARFGVSCPPFTDYVQNLVAFYRRHVEKITRGAMI"
     gene            complement(7372..8151)
                     /locus_tag="PLP50_02660"
     CDS             complement(7372..8151)
                     /locus_tag="PLP50_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPA50480.1"
                     /translation="MSPDPIPYDAPVVERGFGFFETLLLVGRRAVLWEAHLRRLLATL
                     GALELPAPSSEAVESASRAALGGVQDGDDERALRLSWIAIGHDVEEPASWRLDASVRA
                     IPPNTLARRQGSRVLTAPERFRRDSSGFKSTSYFGAVAALRHARKAGCDEALFREGDA
                     YVEGSSTALVAWNGGRLAGLGPGGLPSVTAEAFLEGAGERRGVGRRELLAGGFLLGSL
                     TTAAPILFLDGEECARPPAMLRAIEAFRERLRTDAALQKPL"
     gene            complement(8148..9335)
                     /locus_tag="PLP50_02665"
     CDS             complement(8148..9335)
                     /locus_tag="PLP50_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPA50481.1"
                     /translation="MPSPSVLLRGPGWKLPGPLELADPLEVLEVPAARPEGLPRLLER
                     LDAVSRSGRPELVAAGFLTYEAGVALEGSTALFRPPPTPLAWFGLFDLRAAAGARTDR
                     PGPSGRPGPAEPFGPPAEMTPEEWSAAVSEIRAGIARGDVYQANLTRRVVRALPVSAR
                     RLAALLDADNPVPYAILVDTGAVAVVSNSPELFLEVDLRSRSVASAPIKGTVARTAGG
                     SEEAARALLLSSEKDAAEHLMIVDLVRNDLGRVCTPGSVHVPDLRAVRSYRHLHHLES
                     TVAGSLAPEEGISGLLLATLPAGSITGAPKRSALGFIRRLEPAPRGPYTGAAGFVRGD
                     GTAVLNVAIRTAIVTGGVVAYHAGGGIVWDSDPRREWEESETKSREFFRALAAAAGAS
                     EVR"
     gene            9356..9775
                     /locus_tag="PLP50_02670"
     CDS             9356..9775
                     /locus_tag="PLP50_02670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPA50482.1"
                     /translation="MSEERWVETGVKVRYAETDAQGVVYHGSYVVWFEVGRTEYCETA
                     GYPYPRMEAEGVQIVVVDLSARFRRPARYGDVVRVATRLAAVRSRGCTFAYRVFLPDG
                     GLAVEGETVHLFVDAATGRTIAAPEPIRSALAAFAAG"
     gene            complement(9780..10271)
                     /gene="coaD"
                     /locus_tag="PLP50_02675"
     CDS             complement(9780..10271)
                     /gene="coaD"
                     /locus_tag="PLP50_02675"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPA50483.1"
                     /translation="MRIAVYPGSFDPMTNGHVDLVRRGCHIFDRVLVAVLCNTEKAPL
                     FTVEERVQMTRDVFRREPKVKVKAFSGLLVDFLRAEKTNIVLRGLRVVSDFEYEFQMA
                     LMNRRLDPEVETVFLTPKEELSYLSSRLVKEVHRLGGNVSELVPSLVYRSLQKKLPNG
                     KAT"
     gene            complement(10285..11124)
                     /locus_tag="PLP50_02680"
     CDS             complement(10285..11124)
                     /locus_tag="PLP50_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50484.1"
                     /translation="MTRHCVRRRLLVLALFPALALLAPVLARNRPADAPLAHQLLTPV
                     PFDPDATDLAARLRPPDGTHLLGTDDLGRDVLSRLLHGARVSVLVGLAAAALAFAAGT
                     LLGAAAGTLGPGVDRAVLVLTGVVQAFPALVLVAAGAALVPPSRFTAALLIALTAWPE
                     AARLVRAEALRLAASPYVEAAWAAGASPGRVVLRHVLPGAVAPALATLPYVVGGAVLL
                     EASLSFLGLGVPPPTASWGRALADARESLTSAWWCVVPPAAALFLLVLAARKAGEALT
                     ESR"
     gene            complement(11121..12068)
                     /locus_tag="PLP50_02685"
     CDS             complement(11121..12068)
                     /locus_tag="PLP50_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA50485.1"
                     /translation="MTRFLARRALGAVGTLFGVLVAVFLLLSAAPGDPARLAAGGLPG
                     RRPASEEALRAFRAVHGLDRPVAARLGAWLGSAARLDLGRSFRDGRKVTERIGETLPA
                     TLSVNGVALLLAALAGVPLGIAAARRPGGRADRFSGLLLDALYSLPSFALGLLLLLLF
                     AVKLRWVPVFADPSAGPAGLALPALTLALVALAPIARVVRESVRTALRSPSAIAGRAR
                     GESPREETLRALRRSVPAFAGLLASLVPQLVAGSVLVERVFAVPGTGQLLADSVFARD
                     LPTVLGLTLASGVAVVLATLLADALAAFADPRLVDVEEG"
     gene            complement(12106..13671)
                     /locus_tag="PLP50_02690"
     CDS             complement(12106..13671)
                     /locus_tag="PLP50_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA50486.1"
                     /translation="MKRLALASLLVLSACGGPVGKDQSLVLHRRLEGEPQTLNALTAT
                     SDPENVVIALLQRNLLDYDEDLTLVPGLAESVEADESRLVYTIRLRSGARWEDGTPIS
                     AEDVKVTLEALLDPKTPALYRRSLFADLERVEVLDERTARATFRKAYAAQRDAFNMPL
                     LPAHVYRGTDVATNPANRRPLATGPFRLASWDGPTIRLVRNTQYFGAAPAYEQVVLRV
                     VPESSAAFQGLLAGALDETRLNAAQRPEAAKAGTIRETVYDEIAYTYIAWNNRLPQFS
                     DARVRRALTMLVDREAIARELYAGLAKPANGPLPPGLWPHDATLRPLPHDPNAAAALL
                     DEAGWRKGKDGLRAKGKDRLAFALAFGGASDRQRQVMEVAQRAFGQAGIEAKLSPMEW
                     GAFVERVDTGDYEACSLSLNLDPNPDLRPNWHSSQVPPSGMNHAFYRSPRADALMDEI
                     AATFDRDKARSLYAELQKVIAEDQPFTFLHTVSVAWGVRDRIENVTSSPIGLWLFWPG
                     GAAWQPARGAKPI"
     gene            complement(13668..15929)
                     /locus_tag="PLP50_02695"
     CDS             complement(13668..15929)
                     /locus_tag="PLP50_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50487.1"
                     /translation="MNLTSNRALAASLALALVLPAAPPLFAATRLSTFGSARDFLAGE
                     LDGTTLTSDGRLTLGPVFSPPAWPEEAAGAVVFGAAADATGRVYVATGGGLGRLFVSE
                     PDGSVRLLFEADAPNVTAVAVGPRGEVVCGTSPGGRLWRIDSKAMDASKAGSLAGETG
                     EAAIWAMTVGPDGTVWAGTGTKGRIWRAGADGKASLHAELEDTHVRSLLLRADGSLVA
                     GTSDRGLVVAVAPDGSVRTLHDFGRPEVVALAPAPEGGLYAVVTSVAVPTLAQQRSEP
                     RQTPARAGAAPPPAPGGAPEAVPQGSVSVTSTTSPVRPPAGAPSGREGNAEVVLVAAD
                     GFVEPAWILPEETVYGARWDEGRGALLLATGPKGRVYSLKGRQLRLEAQAGERLVVAS
                     PAVPGGFAIVASSSPAVLRPAGATGRGTYLSAIRDAGRLARFGTIRLEGTVPKGASAL
                     LSVRSGNSGTPDGTWSGWVRAGSSGEAAPPAARYFQWRVELAASPRGEAPVIERVETS
                     WAERNARPIVENVSVLEPGAVFARSGAASGPAVLSVTNPDENGAFAGLEPEKPAETPG
                     KKLFRKGFRTVTWKGTDPNGDTLRYDLEVRREGSATWFPIRRDLEDAFFSFDTAPLPD
                     GRYRFRVTASDRAANVEGEALSTSEESDVALVDGTPPSLVRSDAREEGGFVVLRVKAV
                     DALSPIVRAEGTVSADRWRPLAPADGTLDGREEELTMKVPKPGGPAFLAVRVVDAAGN
                     GASISVEYPKELR"
     gene            complement(15926..17764)
                     /locus_tag="PLP50_02700"
     CDS             complement(15926..17764)
                     /locus_tag="PLP50_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HPA50488.1"
                     /translation="MWVVTLATALLGMPARSAEILPLEGVRTGMKGHGLTVVRGRDVE
                     RFEVEVVGVVRNPAPARSVIVVSLSGLGLEDSGVVAGMSGSPVYLDGKLAGAIASGWG
                     FSKRAIAGVTPIEAMLSIVPETPARVAAGAVSGPPLADRATFLARLASSSDADRVAEL
                     RRAAFGSLPTSATGRGGAALLSFQASGVPASTVESFRDALSRLGASEANLALLGTGDA
                     PLPSDAPSGEPRRLVPGGAVTAYLVRGDLSLGATGTVTEVFPDGRFVAFGHPFLGSGE
                     VELPVAPAEVVTVVSSLFQSFKLANGGEALYRLTNDRDTGVGGRTDRPAQTVPVSVRV
                     EAGGGPARDFRFEAAAHPKLLPTLAGLVADASMTAADPTARERRLAFRIALDTAAGEI
                     AYEDVATGGRAKEVAVLTTVALAAAVADNDLEDPGVRAVRLRFTSASDEQRLRLVDAA
                     PGTRKVAPGGTLSVAIRLADRRGEESVRVVRLRVPAGTPEGRATLVVADGSNATALRM
                     QLDPSEPRTLPQLASFVSRIVPGNRLLAGLLVATRGAATRTETIEALPPTALALLAGA
                     REQGETGIADVEARLVAEEIVPFDRPVSGAVRIDVDVERPRSRGTR"
     gene            17838..18533
                     /locus_tag="PLP50_02705"
     CDS             17838..18533
                     /locus_tag="PLP50_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPA50489.1"
                     /translation="MTDTGGANIVQLFLAAGWTAKAVLLILALFSFGSWAVIVGKLWS
                     FSRADAHSGRFVAHFRKSRKFADVLGVCEQYPSSPLVGLFRAGYAELDAQVKAGRDVA
                     PADSGRLFVKSLPAIERALERAAGVESNRLTRRLPFLATTASATPFIGLFGTVWGILG
                     AFRQIGVTGSTSIVTVAPGIAEALVNTAAGLVAAVPALIAYNYFNGRVKVTRSEMRDF
                     ALEFLNLAERHFT"
     gene            18553..18975
                     /locus_tag="PLP50_02710"
     CDS             18553..18975
                     /locus_tag="PLP50_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HPA50490.1"
                     /translation="MAVKIDDDLEAYSDINVTPLVDVMLVLLIIFMVTAPLLHQGVEV
                     KLPKSVAQNLPKTPEDPLILSITRKEVYYLNETPIPKQQLRERLTLILKNRKDRAVYL
                     KADRNLPYGVVVETMDVLNRLGVESLGMVTELRSEKGP"
     gene            18972..19733
                     /locus_tag="PLP50_02715"
     CDS             18972..19733
                     /locus_tag="PLP50_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPA50491.1"
                     /translation="MTVSEVLEERNRRPDAPHGLAVALSLAAHAAFVVFLVLISQPRK
                     LTTPPLMSLPVRVVSPGALARLSGSPGRPAARPQTAAPRRRVIEKIRDEAPAPSAEAL
                     PLPGKPKKGEAPKPAPRASAPGPRPTASDSAVELPSAGNGGLGGSGSPGLSSFGASLS
                     GFDSDFPFSYYAEQIQALIGANWLKPDVAEGTACVVTFRIQRSGQVTDVKVETPSGLA
                     FYDRAAARAIYSANPLPPLPPEFRGEQLGVHIRFQ"
     gene            19754..21094
                     /locus_tag="PLP50_02720"
     CDS             19754..21094
                     /locus_tag="PLP50_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50492.1"
                     /translation="MRLLSFATGLVLVVALAPIAGQQPAPTVAPPAQPTDISLVLSQE
                     GGRRVALAIPSPKAPVSDVVERDLVEPFRSALTGDLGSSPWFVLADPALHPKGFRPPA
                     TREEGDAWIASGAQFLLDTQIQAEGTNVVVTAQLIDLRTLRPILGKRYSAAGVSIRRV
                     AHTIANDVVRQFTGRPGPFLTRIAFVSDRDGGRGKEIYVMDWDGENERRLTFHRSLSI
                     APDWSYDGSKIVYQSFVSTPPGLYWFSLSGGTRNKVPVPTELNSAPSFSPDGKMVAYA
                     GSVRGNPEIFVVNLDGSGHRRLTNSSAIDSSPRFSPNGREIAFTSNRQGTPQIYLMDL
                     EGANVRKLTLTGNWSDEPAFSPDGGRLAYACRNEGDFQICVLDLTTGRTFQISSGPGT
                     NENPTWSPDGSRIAWELQRGRSTQIVSAGIDGSGFKVLTEAGNNGYPVWERTMD"
     gene            21190..21747
                     /gene="pal"
                     /locus_tag="PLP50_02725"
     CDS             21190..21747
                     /gene="pal"
                     /locus_tag="PLP50_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPA50493.1"
                     /translation="MTKPFRSLGLVLAAGLLVTAGCCTKKPAPAPAPEVKVEPTPTPV
                     PVPVEEPIAAPVAEPVAESLPADLEELNKKGYLKDIFYDYDLYDVRADQRDAMAANAE
                     WLKKWPTVKVQVEGHCDERGSNKYNMALGDKRANAARDYLVSLGIDASRITTLSYGEE
                     RPFVEGQTEDAWSQNRRAHFVVTAK"
     gene            21744..22622
                     /gene="ybgF"
                     /locus_tag="PLP50_02730"
     CDS             21744..22622
                     /gene="ybgF"
                     /locus_tag="PLP50_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPA50494.1"
                     /translation="MTALRIDRLRRSAPLAGALLFASALGGCVSGSDIEGLHRHVTDV
                     EKQVDKLSQQTSSKDEVEKLNQNLTKQVATILRSNAETGTRFDELTRELQSLAGKLED
                     SNRRLAQLSQQLAETQAKLDAGSPATLTVPVAGFHPGAGAPPAAVVPAAPTGTPAPKP
                     PAGRAGPADLYNAALGDYQRGRFDLARQGFEEYVETYPRTDLSDDALFWAAECWTAQK
                     KHREALGSLERLLKEYPQSDKASSAHLRKGLAHLELGEKAQAIVQLQFVVHEYPTSDE
                     AKSARQRLRSLGSDSR"
     gene            22686..22949
                     /gene="rpsT"
                     /locus_tag="PLP50_02735"
     CDS             22686..22949
                     /gene="rpsT"
                     /locus_tag="PLP50_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPA50495.1"
                     /translation="MANTKQAKKRAAQNDKQKARNRAVKSETRRVVKATRAAAPEKAK
                     EMLPKAASSLDVAARKGTIHKNKAARLKSRLATGANKKAAKAD"
     gene            complement(23099..23617)
                     /locus_tag="PLP50_02740"
     CDS             complement(23099..23617)
                     /locus_tag="PLP50_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPA50496.1"
                     /translation="MRRRAFVAAALAAALAGCGYSLVGTGRGILPEGASAVFVETFVN
                     DTTRVGLEQRLTDAVLRELAGRARLSPVTDRSAADVELSGRIVSYQVNPVRFDDQGRA
                     LEYEIAVVTRLKLTDRKTEKALFENPSFLFRQPYPVAATSSSYVDVENAAIEAMARPF
                     ARSLVTTILEGF"
     gene            complement(23614..26085)
                     /gene="leuS"
                     /locus_tag="PLP50_02745"
     CDS             complement(23614..26085)
                     /gene="leuS"
                     /locus_tag="PLP50_02745"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPA50497.1"
                     /translation="MDAREERTALEKKWQARWDADRAAFVDTHDPSKKGTYLLVMLPY
                     PSGDRLHVGHARTYFLTDALHRFLRQKGETVFCPMGWDAFGLPAENFAIQRGIPPRES
                     TLANIAAMKEQFAAWGVLYDWTKEVTTCEPEYYRWNQWLFLRMLERGLAYRKKGTVNW
                     CPSCQTVLANEQAEGGTCERCKSAVEQRELEQWFLKITDYAESLLAGLDQLGKWSEKV
                     RTMQRNWIGRSVGADLEFEVPKLGERLEVFTTRPDTVYGATAMILAPEHPAVPRLIEG
                     HPDRAALEEWVKTIRSQDRVQREAEGAEKDGRFTGAYAINPFTGEEMPIWIANFVLAG
                     YGTGAIMAVPAHDERDHEFAKKYGLPIREVIRRPEGADASIACWSGEGTMTASGPYDG
                     LSSGAGREAIAAEATKRGIGGARVRYRLRDWLISRQRYWGTPIPMVKCPACGWVPVPD
                     DQLPVVLPKDAPFTGRGGNPLEKVEAFLKTPCPKCGGEARRETDTMDTFVDSSWYYLR
                     YLDPTNDRAPFDPAVIARWAPVNQYIGGIEHAILHLLYARFFARVMKDLGLVAFEEPF
                     SALFNQGMITRMSPGGRVEKMSKSRGNAVSLDPLIAAKGADAVRAYVLFLGPPESDAE
                     WTDDQIAGPERFLFRVKALVESWLKAHGTTPHAPAAADSPAARKRHIAVKSVTHDFET
                     FSFHTAVAHLMEFSHHVAGLVGDAKADPGETRASILALLALLHPIAPHLTEEWNERMG
                     ETRSLLVSGWPAFDPALAVEESVTIAVQVSGKLRGQVVLPRGASREEVLAAAEALDGV
                     AKWLEGRERVKVVFVPDRLLNVVVK"
     gene            complement(26120..27184)
                     /locus_tag="PLP50_02750"
     CDS             complement(26120..27184)
                     /locus_tag="PLP50_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HPA50498.1"
                     /translation="MRVLVVEDSAYQRLRIVEALRASSAITEVDAVGSGEEAIRRLLT
                     TDYGLVTLDLGLPGMDGNAVLRWIMANRPLPVVVISADQEERSALAALEAGALDVIAK
                     AGSQPESLARWKRRLAEVVDGVRQLSIESLVRRSKRVGAPPPSGAVPRPRRVPVALGV
                     PPALVVAASTGGPPALRELFAGLPPQEAVVGVAQHMPPPFTRSLAQRLASGTGWDVRE
                     AIHGAEATPGVVWIAPGGAHLELSHAGERAVLVVRPHGPSVRWCPSADLLFASAAAAF
                     GRRVLGVVLTGMGDDGAEGCRAIALAGGTVLCESAETAVIAGMPDAAARAVPAARRFP
                     LSRLAAEIERRLGEAAGAAA"
     gene            complement(27189..28013)
                     /locus_tag="PLP50_02755"
     CDS             complement(27189..28013)
                     /locus_tag="PLP50_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPA50499.1"
                     /translation="MTGRPLELAESVFRLYQDLLRRRTGFVVSDSQRVRVESRLAAEA
                     RGAGSFYQLYGRLRNEPQDSPLFGRLLDAVVVGETYFFRDPASLRAFSEEIVPERLLS
                     AGQDGALDVWSAGCATGEEAFSLSIVLHEKGVLPGRKVRILGTDLSPAHVRRARAAVY
                     GPQSLRATSAERRREHFEAAPGGLFRLKPPLAAPVLFETRSILDEPAFGTRFDVILCR
                     NVLIYLDEEARARVVEVLSSRLKTGGYILLGASDALSVASSALTLVRLANDVVYRR"
     gene            complement(28010..30226)
                     /locus_tag="PLP50_02760"
     CDS             complement(28010..30226)
                     /locus_tag="PLP50_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPA50500.1"
                     /translation="MSDDRIRELAARAASKDATERWTVAGELGRIPGEEAESVLLALL
                     KDEDYRVRERAVAALGRRFTPRVASACAVALADDDDAGHRAAGLALLVRGGAAGRAVL
                     LGALQHPSADVRISAAAALPGPAPGPETVTAIEAAARREGDPNARAALLLALGRTGRR
                     EAIAPLLAALEEGNLWLQVHALEALGDVGDPEIAPRILPLLTNANLRHGALHALARLR
                     SPVAGEPLLRRAAAGEVDVFLVAALRAALESASATTLAGLRPLWPEAGPTLLALLEDP
                     EEDAALRTDAAHVLALLDLPGAALAIVTHGPFRDGYAALRALPRARREEALLCVLQVA
                     DPEPALELLDAHRGGPEAPTLLPLLVHPSATVKAAVLSCVPAGLVPIPDLIDVLAEDD
                     PETALAAALALASDDSGEPLDRLPKRRRALLDRAGGPDGPGRVAALAALAGTDGPDVE
                     AAVRGALSTPDPAVREAAVTAAARGRRIGSHEIVACLSDEIATVRAAALRGLALRAER
                     GEALPGVTWRDGLVFLVDDPAAAAAAGAAVIALAGAERPRLVEEMLVQEPAVRIAALE
                     EIEKTGDPEAARSAVAALGHEDPETACAAVRALSVADEAVAAEALATALADERAEVRA
                     AAAETVARRPPGDSRSALSGAVATALVVESDRAVLRSLLVAASGLADEAMVDPMTLVL
                     SQESIPAEADDAAEALARLAPEACRRAWMQAPARAERRWARALATAARAGRSQRST"
     gene            complement(30223..30636)
                     /locus_tag="PLP50_02765"
     CDS             complement(30223..30636)
                     /locus_tag="PLP50_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPA50501.1"
                     /translation="MSDDGLLLAFRAGGERFAVPLERVREAARPRRIAPLPGAPPEWS
                     GVALVRGEALGVIDVAAAMGLPRSPAKPAPLVVVLYGTDHALLVDSIDGVETCPADRV
                     APPPPGRGALRGIVAEESGFLSLLDVDRLAAKGEG"
     gene            complement(30633..32669)
                     /locus_tag="PLP50_02770"
     CDS             complement(30633..32669)
                     /locus_tag="PLP50_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50502.1"
                     /translation="MTRPKRPGDASSGEFLAEAEMLLEEAGCGIDALDAEGDDPRPEK
                     VNALFRTVHSLKGVAGMVGYSGIADAAHALEALLDDLRMGRVPPSPAVRGGVRDGLDA
                     LSTLVARVAAGEESPRLETPLKDRLEGLVRPAEPREAASLRLPPELDASLSDYERHRA
                     SEAGKRGKALVLVDLDLDFDSFDAGLRNAMNEASAAGELIGTFPGTAADPARMAFRLL
                     VALPPGSDVAALATRCSARDVVGVSAGTAAPEPATEPVRLPATVVAEPAPPEPGPEAA
                     PQVARGVVRVPLEKVAALVDLAGELALARSALGRTLGQALAGAPDRGARFEAQRAFAR
                     LDRLVATLGPAALALRLVPVETLTTRLARAFARTAAALGREADFVVQGVETEIDKVLA
                     DELGDPLLHILRNALDHGIESPEERAAAGKPRRGRVALTVAPRGREVVLTFSDDGRGV
                     DAAGIVAKARQTGLLGPSDPDPAEPFALLFRAGFSTAREVSEISGRGVGLDVVREKLT
                     ALGGRVSVRSVPGSGTTFEVVVPMTLVLLESLVVRVGDVWFALPGENVRRTLQAAEGA
                     IEVIDGRPCFRDGDEALPLARLDALFDLPRRPDAGGAEPVVVTEDGRRAAALVVDAVE
                     GVWDLLVKPLADSVPRPREIAGAAEVPGGEIALALDAGALVARVHSASASGRRA"
     gene            complement(32666..33088)
                     /locus_tag="PLP50_02775"
     CDS             complement(32666..33088)
                     /locus_tag="PLP50_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50503.1"
                     /translation="MTASGPIRRHVLVVDDSGPARLLVRQALEGWARRTGHKVDVVEA
                     ENGFEALRELPRSPIDLVLTDVNMPTLSGLELIRMIRSRPEQSALPVIAVSTERDAED
                     AARARKAGADGYLGKPFDVETFDRVLGEVLGSKAPEEP"
     gene            complement(33085..33927)
                     /locus_tag="PLP50_02780"
     CDS             complement(33085..33927)
                     /locus_tag="PLP50_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPA50504.1"
                     /translation="MNLSPLALAFLLLSGPERAAVETARYRVEGIEPWRAVRPVEAPG
                     YRVVLSSPDGRTLEATVEVDGAPLRDTAPYPVDPRTLPAEARALLAEPLDADDELEDL
                     SRILLRGATTQLEAVERVVAWVSKRIRYESPSLLAETAASCHRSRRGSCVGRSLLAAD
                     LLRRGGIPVRQVTGILTARTPSELTDESQQHYSDTISGVRHRWIEVFVPGLGWVPSDP
                     GGLANMLTARHLALPAAPPDGFGLTILARGPTLAWKVRDGSFARPRLRVVEAVDLPPG
                     EPQR"
     gene            complement(33924..34385)
                     /gene="nusB"
                     /locus_tag="PLP50_02785"
     CDS             complement(33924..34385)
                     /gene="nusB"
                     /locus_tag="PLP50_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPA50505.1"
                     /translation="MSGRRRRARELALQLLYQRDIAGTSPEEMFARTDEYRNAAPEVQ
                     EYASRLVLGTLGRLSELDELLGKQSDHWRLGRMPVVDRNLLRVALYELLHEDETPDPV
                     VIDEAVEIAKKFSTPSSAPFINGVLDGIRRNLFGAREASLRRSRPAPAGSR"
     gene            complement(34395..34877)
                     /gene="ribH"
                     /locus_tag="PLP50_02790"
     CDS             complement(34395..34877)
                     /gene="ribH"
                     /locus_tag="PLP50_02790"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPA50506.1"
                     /translation="MNVLEGQLRADGRRFGIVASRTNDIVVSRLLDGALDAFRRLGAA
                     EGAVTVVKVPGSWEVPLALRDLARSGGFDALVALGAVIRGGTPHFDYVATEVAKGVFQ
                     VGLELDLPITFGVLTCDSLEQALERSGAKGGNKGFEAATAAVELADLRARLRAGAPVR
                     "
     gene            complement(34885..35946)
                     /locus_tag="PLP50_02795"
     CDS             complement(34885..35946)
                     /locus_tag="PLP50_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPA50507.1"
                     /translation="MSESPKVVVTGSVAFDYLMRFPGKFQDVVVPDRMHRLSVSFLVD
                     EMRRVRGGVAPNIAYGLALFGMTPAIFATAGQDAADYRAWLGAQGCDVSGMKLCDDVF
                     TASFFVSTDEEQNQIATFYAGAMAKTSELTFRSFAPGSVALALICPSDPTAMRQHAAE
                     CRELGIPFVYDPSQQVARLDGDDLLAGLEGAAMLIGNEYELGVIEKKTGLSEAELAAK
                     VPVLVITRGEEGATIALRGGSPEEPGGARTVRVPAAKLRTEALDPTGVGDAFRSGLVA
                     SRLRGLPWEEAGKVGAVAAVLCLETLGPQPRRWSLDELLERYAESFGKPLVEPLAERL
                     RGLAGTEASRPAARFSLLD"
     gene            complement(35943..36803)
                     /gene="mtnP"
                     /locus_tag="PLP50_02800"
     CDS             complement(35943..36803)
                     /gene="mtnP"
                     /locus_tag="PLP50_02800"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPA50508.1"
                     /translation="MRKRIGLVGGSGLYALPGLVVAEERALETPFGDPSDAFVLGELS
                     GVPVAFLARHGRGHRFLPSEVNYRANVWGFRKLGCSYLISSSACGSMKLEYRPTDIVL
                     PDQLLDLTKGRTSTFFGEGCVAHVSFSHPVSEHLLGLLAAAAKETGAVAHRGGTYLCM
                     EGPQFSTLAESRLYRSWGVDVIGMTNATEAKLCREAEIDYATMCLVTDYDCWHEEEAP
                     VSVEAVLAVLRRNAETADRVLARAVASLDPDRPPDCDGAMRHAVLTAPSAIPPAARAR
                     LALLMERYWR"
     gene            complement(36800..37750)
                     /locus_tag="PLP50_02805"
     CDS             complement(36800..37750)
                     /locus_tag="PLP50_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPA50509.1"
                     /translation="MSEKIRTGIAGCGYLGRHHARILTELPLSEPVGFVEPNDDMAAE
                     IESKHGASGLVRCRSVKELLERGASAVIVATPTSTHAEVAEELLSGGADVMVEKPMTV
                     TLEEADRLISIARAKGRVLQVGHIERFNPAVVAVLPLVTEPKFIEAHRLGVFVPRALD
                     VDVVLDLLVHDLDIALEIVKKPVTAIHAVGVPILSKKVDIASIRIEFEGGCVANLTAS
                     RVSTEKTRKFRFFQPDWYFSIDTQKRDVTAYRLDRTSGPPRIMEWPVEVVPTDPLTAE
                     DGAFLEACRTRTEPAVTGEAGRAALKLALDVQEEVARRIG"
     gene            complement(37750..38388)
                     /locus_tag="PLP50_02810"
     CDS             complement(37750..38388)
                     /locus_tag="PLP50_02810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPA50510.1"
                     /translation="MPVPVRAVLFDMDGVLVFSEDAWFSVFNETLTCFGHRPVSRAEF
                     DAIYGNGAAADRDAYMPERSVAEIDSAYARLFEEHLAEIRPNAAAPAVLAELRRRGIR
                     TSVATNTTAPLARRVLALHGLLPLLDATASADEAGAGKPDPAVVRLAAERVGTPLDEC
                     LFVGDSRFDAAAAIAAPVRFVGLGHGGAERIERLGELLDIVGIDTAPINSRV"
     gene            complement(38439..39401)
                     /locus_tag="PLP50_02815"
     CDS             complement(38439..39401)
                     /locus_tag="PLP50_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPA50511.1"
                     /translation="MRLLVVKLSSLGDVAHAVPSVAALARRFPRAEIDWLVEPPAAPF
                     VAELPWVAETVVADVRGAFAAGWAELFAERERVAARLVARRYDLVVDLQGLLRSSLWT
                     VWSGADRRVGRGRWPWLHRRVPMYDHRRTPHAIENTARAVEALGASVREGWEEAQRGL
                     APLAARLRAAGTREAERLGLPRRFTAAFPMSSGLSRSIPPEWLERWPGPPLVLLGDER
                     LAGYRPRREGILNCAGTIPLLGSLGLALAAERVISADTGPGHMAALLGAESLSILGPT
                     NPARAGLRGPRARSAGAPCGGCESKRCRSATRCLADAFESAGVR"
     gene            39462..40274
                     /locus_tag="PLP50_02820"
     CDS             39462..40274
                     /locus_tag="PLP50_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA50512.1"
                     /translation="MPRVAVVIATHRRPALLAEALSSVAAQTFRDVETVVAEDGGTEE
                     TARVVAASGAPGARHVPLAFCGRPGPVRNEALRRVSSPLVAFLDDDDLWLHRRLEAHV
                     AALEAAPAAGLVFGPIRRFGDEEGTWPRHVPPRVDLARLLRGNCVPLSSVLARQEALE
                     RAGLFPDRAELAQDYELWLRVARSHVLVGHPEPLVRYRVHTGGISRKKALEVEAVAAI
                     LTRLESEWDLPPRLLAPARRGLLRSRARLAPGLREAIRLRALSLAPGPFLRG"
     gene            complement(40329..41432)
                     /locus_tag="PLP50_02825"
     CDS             complement(40329..41432)
                     /locus_tag="PLP50_02825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA50513.1"
                     /translation="MRATLLVDRWDASRGGQEGYLVALGGELVRRGVGIRVLCREGAG
                     KGDGTSVEALVPPGGGVFGERRFLAAAARRLEAGEGPVLAPRLVPFATHVQLHGGLFA
                     DALEAERESMEGVRRALFDVGTALNGRRRLLLRREREALAGACPKIMVWTEALRSRLR
                     VLGVPDGAIRVAPPGVDLALFSPDPDGFRPRAAEFLFLAHNPRLKGLRTALEAVAVAR
                     RRGLAARLSVVGRVPRDAWRRIAARLGVAEAVTFEGPLSRGDVVRKLRASTGLLHPTF
                     LDPCSLACLEAAACGVPVITTRRNGAVERLGPAGAALVVDDPRDVEGLARAMEEIAEP
                     TRRAELREAALGFRPSLDAQRHLDDVVDWLGVG"
     gene            41644..42432
                     /gene="lpxA"
                     /locus_tag="PLP50_02830"
     CDS             41644..42432
                     /gene="lpxA"
                     /locus_tag="PLP50_02830"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPA50514.1"
                     /translation="MGRHHTALVDPGARLGAGVEVGPFAVVEAETGIGDGCELGAHSV
                     VKRYTWMGARNRVHEGAILGGAPQDLGFDGGPSRLEIGDDNRIREGVTIHRATAPGGV
                     TRVGSGCFLMAYSHVAHEDVIGDHVILANNVALAGHVRIDERAFLSGGVVVHQFCRVG
                     RLAMVGGNTKVIKDCLPFVITDGVPARARGLNVVGLRRAGFTASQLRTLKEAYRLLLR
                     SGLPLAEALEKLEALGDPLADELGAFARDSKRGFHRSSREIDLA"
     gene            42512..42997
                     /locus_tag="PLP50_02835"
     CDS             42512..42997
                     /locus_tag="PLP50_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50515.1"
                     /translation="MDSFPPSGSPRGFDAARAAHPTRVLRIEEVVDLLELTHAAEVIR
                     GYRERMRAGDLFPPVSVVRLFGKWLVADGHKRYVAWTGLGEKEIVVEVWPLGRWLADQ
                     AGQARRNARKNGRIAARLFVDPRESLRLARTTAGHWRRVARSLLRRAGRRGKRPASLP
                     A"
     gene            42994..44763
                     /locus_tag="PLP50_02840"
     CDS             42994..44763
                     /locus_tag="PLP50_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50516.1"
                     /translation="MSDGILAPGAGRRLLLRIARDAAVERSRFAGAAVALATMAGAQL
                     LLTWLVKEWLEGPLTGGSLLPVRALLLEAAGATLLLAAAVVLSRYLAASVNQRLLERL
                     RDRAARRVLASRALEVRGRPAGELLSRVFSDASSLSGFVETLLKRLVGDGLDALGSIA
                     VAFFVEWRLALGAVVLAPLLGLLLDRLGRVVRRKGSVAQRKLGELHAVFSEQLSGLTT
                     IQGYDAAGREADRFAATNATYRRSVLSAELWSATVLASIFLVTGLGFLGAIGWGSRLA
                     LANELTPAGLLAFCLFAARTVEPLRRLADVHATLQRTLAAAARVYEVIDFGFLERQGG
                     TPLPRPVRGRLALEGVGFRHEPSRPLLEGVDLRVEPGETVAVVAASGGGKSTLAAMLA
                     GFLSPDVGRLALDGVELREADLRDLRRAVRLVEQEPFLFRGTLADNVLFGRPEASRSE
                     VVGALGLCGLESLVAAIPGGIDGSLLEAGRNLSGGQKQRIALARAVLTDPPVLVLDEA
                     TSALDGETEGRLLDDLGPWLARRTVIVMAHRLSTVARYPRVIVLVDGRVAGDGPASRL
                     LESCLPFRALFADQVEPVANRAT"
     gene            complement(44804..46198)
                     /locus_tag="PLP50_02845"
     CDS             complement(44804..46198)
                     /locus_tag="PLP50_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPA50517.1"
                     /translation="MTDVVARRDDAAETPPLGGLPSPEGPSVREPGLSRFHGFVLGAY
                     GVAAGAAVAAARIALLRGAGASERAERLGTAPPPCGPGRLLLHAVSAGEMTAASALAS
                     EIERTCGVKLLLTTGTREGRIVAERIRETCRAVEAVAFLPWDRPGAVRRWLARLAPAA
                     VVTVEAELWPGLFLGARSLGIPVAVASARLRPGEAWRYARAGPFFRPVVDAVAWIGAR
                     TEEDRERFLAIGVSPSRVEVTGDLKLDAPTPAAALPPGWEAWLAIGPPLLVAASTHAP
                     EEELLLAAVKRLRGESAEVRLAIAPRHVARSSEVERLARQAGFGTCRLSGPPGPASIL
                     VVDTFGLLSSLWPHAAIGFLGGTFAPVGGHSPVAAAEAGVPLLAGPSLEGVRDVADAL
                     RHAGALVEVGKDGAVEELAAALARLLSDDGDRLRRGEAGRRALGSLRGAAARTATRIL
                     GLLASARHTGPEPL"
     gene            complement(46195..47853)
                     /locus_tag="PLP50_02850"
     CDS             complement(46195..47853)
                     /locus_tag="PLP50_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA50518.1"
                     /translation="MSRSHLSQERFAPRAAFALAAAVVAALLLLAPRTTLWDRDESRF
                     ARSAVEMLSSGDFLVPTFAGELRPHKPPLLPWLMALSTSVLGPTEVAFRLWSALAHGA
                     SVLLTFAAGRRLAGGTSPLLGAALLALSPLALLEGAAATADALLLAGLTGVLAVFADA
                     ATGGVRPRHLVLGALASAVAQLAKGPVAFALPLLGLGGALLLVPGAFRERRRLAVLLA
                     ASSILGLLPVLGWGLLADAATNGRLLSEAIGTHVIGRTLAPMEGHGSGLLVGPLYYGA
                     VLLVGFSPFTLFLPSAWARVRSGGGLAPGARAILLGSVLAPLLLFSVVATKLPHYLLP
                     AFPALALLAAEAAGAGERRGLVAGGLLLAPVVVAALAALTAIVTVPPLAALRAPAAVA
                     IVAIAAEVALALVLLRSGHTRAAAATLLAGTAVALVATAGFGLPALERWKPVPRLAAE
                     ARRAVGDAPAATLGFEEPSVFVYFGPGPVERLASEADAARWARGEGPGLLVTTRAGLE
                     RLVAREGLLPLVPVARQGGMNVSKGEPVELVALARGGPWTMAPR"
     gene            48264..48854
                     /locus_tag="PLP50_02855"
     CDS             48264..48854
                     /locus_tag="PLP50_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50519.1"
                     /translation="MTVYQTEGISAYRTRPDGTVQSLFSNEAGTLFTFTQTPYVPPAA
                     AGGARALASSASGTGQLLDQNADGIYETMQVEGTGVPQTRMSLVPRDTTGDGRPDFIT
                     VPWTTSGAGLLGVATATTPQIYFPLTDTDADGWPDTITVQEVGGISTSTGPPLSGAAL
                     AAAAVVPSASTFGLFFFAAAVVALGVKLLRGTATAL"
     gene            48868..49350
                     /locus_tag="PLP50_02860"
     CDS             48868..49350
                     /locus_tag="PLP50_02860"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HPA50520.1"
                     /translation="MRFVLVDQFLSVEPGRGATALKTFRPDDPVFADHFPGFPIVPGV
                     LLTESMGQAAGWALATKVGPDRFPLLMMVERAKFRRLVRPGDEIRLEADLEETREDVW
                     TARTRASVGDERVADARLVFHLVSRPSGSAEFDAWRAGILRETGLGRLVETALSSSAE
                     "
     gene            49347..49544
                     /locus_tag="PLP50_02865"
     CDS             49347..49544
                     /locus_tag="PLP50_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50521.1"
                     /translation="MKRRLGLFLLLSGTFVAARVLVARAVDDRNPETRLLALHAVAVP
                     LAQLALFEAAERLRARRKGTA"
     gene            49541..50749
                     /gene="lpxB"
                     /locus_tag="PLP50_02870"
     CDS             49541..50749
                     /gene="lpxB"
                     /locus_tag="PLP50_02870"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPA50522.1"
                     /translation="MRILLSAGEVSGDVAGARLVRALRARRSDLAFFGLGGPRMAEAG
                     VELLRSTNALGTVGISESFRVVPGLARAFASLRRRVAGSPPDAAVLIANDVFHVVLGR
                     WLRARGVPTLSYFPPQVWIWRSLARVFAPSFDEILTSFPDEERVWRAAGASTTFVGHY
                     LGDVLRPATEAERRHAREALGLPAGPLVAILPGSRELEVRRLTPVLLDAARLLHERDG
                     MVRFVLPVADPAFAPRIEAEVARRGLGGVLGTTSSGHDALRAADLAILASGTASLEAA
                     LIGTPMVIAYRLAALTMGVVRSAIALGLIDSDTVGLPNLVLGRRAIPEHIQDRASGLA
                     LSAAAVRLLEDDSLLEEQRQALGEVSRLLFAGGSDARAAEAVLAAAERRPVRGAAFGG
                     AIVREEGSGT"
     gene            50746..51786
                     /gene="lpxD"
                     /locus_tag="PLP50_02875"
     CDS             50746..51786
                     /gene="lpxD"
                     /locus_tag="PLP50_02875"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPA50523.1"
                     /translation="MRKTTLGEIAVLLGGEIDGDRETPIDRLEPPEEAGEGALAVLAD
                     RKALAGIASCRASALVVPPGTEAPGRSIVRVPYPRRAFVALLAHFAPDDARGPGVDPR
                     AYVDPGAEMGEGVWVGPGAVVEAGARLGARVRVHPLAVVGEGARVGDDSVLFPGVILY
                     PGVTVGRRVRIHAGSVIGSDGFGYERDAGGVQHKVPQIGTVEIADDVEIGSNSCVDRA
                     TVGATRIGAGTKIDNLVQVGHNTQVGEHCCVIGQAGLSGSVKVGRLSILAGQSGIADH
                     VTLAEGTVVGAQAGVHRDIGPGIWHGAPALPAAEARRVFPIVARLPELLRRLRELEEK
                     VAALEGRDGPGT"
     gene            51783..52799
                     /locus_tag="PLP50_02880"
     CDS             51783..52799
                     /locus_tag="PLP50_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPA50524.1"
                     /translation="MRERRVVVTGLGLLTPLGTGLGATRLALAEGRCAIAPATLFDAS
                     PFASPLAGEVRGLDPRAYFRVPKALKLCDRRTRFAVCAAAMALEDAGFPAGPCDGLGV
                     SIGTSGSDLGAEELSAALARSEDPALSARDTDAFGEAALAGLNPLWLLVHLPNMTSAH
                     VAIQREARGPNTTVMTGWAAGLSAIVEGAAWIAEGRADAVLAGGADSAVHPFAFAALE
                     QKGLLSGPSPLVPAEGAAVVLFEEREAALARGARLLGEVGAHALSAGTRLAALAADAT
                     PAPSRQLERLAGNLLAAHAPAALVLSLAEGLAGPVAAVAMGPAGEASALLVLPPDEAA
                     RSAA"
     gene            52796..53194
                     /locus_tag="PLP50_02885"
     CDS             52796..53194
                     /locus_tag="PLP50_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPA50525.1"
                     /translation="MNPSPSEIESRVLSIVADALDQPAERVTLDASLVDDLGAESIDF
                     LDIVFRLESDFGLKIPEEEIWQGSFDGVGDDPDALAERLAQLKVRRPGFRWDRVPDRP
                     TRSDLPRLITVGTVVEYLEKRLSGGAASPE"
     gene            53191..54474
                     /locus_tag="PLP50_02890"
     CDS             53191..54474
                     /locus_tag="PLP50_02890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPA50526.1"
                     /translation="MRTRRRVVVTGAGLVAPGADGAAGLWENLLAGRSAIGPIRRFDA
                     SSYPTRIAAEALAPAGAPASVLGEEFGGRGRIWDFAAEAASQALSAAGLAPRGPWAAS
                     AGLSLATGMGTYGHAEVFGAAAAGAARGGFDGKAFAEELRLGLLPHAADRQSPGALAA
                     HLARAAGIGGPLLASMTACAGGTQAVGDAFRWIRRGEADVVLAGASDSEIYPMGLASF
                     CLLGALSRRNDAPERASRPFDGARDGFVLGEGAGFLVLEEREHARARGADVLAEVAGF
                     GSACDSYRVTDPHPEGLGAALAMRRALDDAGLSPDDVDYVNAHGTSTPGGDRVEVRAM
                     KAVFGSRLDGVAVSSTKSMIGHLTVAAGAVEAIVTALSLRDGRVHPTLNQELRDPECE
                     IDTVPEGARRVDLRAALSNSFAFGGQTACLAFVRA"
     gene            54471..55139
                     /locus_tag="PLP50_02895"
     CDS             54471..55139
                     /locus_tag="PLP50_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HPA50527.1"
                     /translation="MKIRRSPPRSLARALGRIYGLLHRTLRLEGILPDGSRVTPATYP
                     FGPEVFAICERDALALAGVLGRARFTTLVAPGRDGDWATEVVTSLGGRVVRGATLRGG
                     AQALAQLLHGLPGDDGPLALVVDGPLGPSGVAKGGAVVCAARTGRPLRPVGAAARRGL
                     VLKRSWSRIWLPLPFSRVVVVVGEPLPVPAGMGRTDRERLATELTTRLAAARSRALEE
                     VAGA"
     gene            55136..55912
                     /locus_tag="PLP50_02900"
     CDS             55136..55912
                     /locus_tag="PLP50_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50528.1"
                     /translation="MRRFLGYWLLLSPAYLVAAALSSWLLLNLVDVTYGAFAAWLLVP
                     VAQALALAATSRQMPLLPAGRPPLLAFVTLASGLAVVAAGLARPLDARWGFAGSGDLQ
                     RLAPRVLAILAGAAFLASCARLRKGRPAVASLGALLLLLGLDAVRPYLATVPGALLPR
                     LGPFLGGLVTWGVLVVLVFAAALTAQRALEETSAWAARLLGASLGVLFGALHGALLQL
                     FLHPWLEKPWSILIPAAVSVAAALAAAAGLAALVGRRESA"
     gene            55909..56910
                     /locus_tag="PLP50_02905"
     CDS             55909..56910
                     /locus_tag="PLP50_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50529.1"
                     /translation="MTRGAPVRALVLARDLFLTLVALPFLVPLRLLPWNAAGRLGSLY
                     GLAAWAVLGTSRRAGLVNLRRAYGPSMSRAEAGRAVRAVFASLGRSLAEGIRFSALPG
                     RADGSWRTLYDAEDPGLEARILADPRPKVFVTGHLGSWEVAMQMLDLALEGRGAAVAR
                     SVDNPFLDALLRRVRRGDGALIEKRGAVVPALAALRSGTSVAVLLDENGGRSGPFPLF
                     FGRPASTRKTAALLSARTGAPIVVGAAVRRGERFLFRLAFFEPPGTGVPTPSDVLETT
                     ERIVRVWEAWVREDPLQWRWIHWRWKTRPGGSEETYTRRDVNAAFAADGPAAREVSA"
     gene            56907..57662
                     /locus_tag="PLP50_02910"
     CDS             56907..57662
                     /locus_tag="PLP50_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA50530.1"
                     /translation="MTTPFPDLRGRVAVVSGGTRGLGRAISLALAASGARLVLGYRTD
                     AAAAEEAARACQDLGAEVRTVAANLVHPEEARGLVAAAGDRVDVLVHSAALGSFKPLL
                     DVKPAQWDLTLAVNARAFLLLAREASERMPDGGRLVALSSLGGSRFIPEYGAIGPSKA
                     ALEAVVRTLAVELGPRGILVNAVSAGLVPTASVKLHPRYEELAAHARAASPLGRLGTP
                     EDVASAVLLLLSPLAGWITGQTLVVDGGASLAT"
     gene            57801..60722
                     /gene="ileS"
                     /locus_tag="PLP50_02915"
     CDS             57801..60722
                     /gene="ileS"
                     /locus_tag="PLP50_02915"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPA50531.1"
                     /translation="MEARDYKATLQLPTTSFPMKADLPKREPERLRRWLDSGLYEKVL
                     AARRAAGAPRYILHDGPPYANGHIHIGTALNKILKDVVVRSKTLAGFEAPYVPGWDCH
                     GLPIELKVDKELGSKKREMDPVTFRKACREYAQKWVVAQRDDFQRLGILGAWEAPYRT
                     MDYGYQATIARSFGEFLAKGLVTFGFKAVLWCVNCKTALAEAEVEYEDRKDPSIHVAF
                     PVPVTEALRALWGEALAGDPELFAVIWTTTPWTLPANRAICLGPEIPYVLARRASEPA
                     KLYVLAEPLVEATAKALGWSDLAAVPGTTRKGAEVEMARPSYRRPFDLDGISFGFLLG
                     EHVSTDDGTGLVHTAPGHGREDHDVVKRSGFPVDDPALCPVDEAGFYDRHVPEPLRGK
                     RVVAAKSPDLDANRAVLAMLEQGDLSGARLLGRTDVAHSYPHCWRCKSPVVFRSTHQW
                     FIDLGALREKALSEIRERVEWVPAFGVNRIGAMVENRLEWTISRQRLWGSPITILRPK
                     EPKDGMPEYYPSADDPAELKLFFDNLVAIFEKHGADAWWDDEAFPADAFVPPASRWKG
                     TAFEKVKDILDVWFDSGVSHAAVLGTSGYGIASPYADPKRPHVLYLEGHDQHRGWFQS
                     SLLTAVALLGRAPYETVVTHGFVVAGDGRKMSKSDGNTVEPDAVIEKHGADVLRLWVA
                     SSDYTDDIRLNQEILTRTAEAYRKVRNTARFLLSNLFDFDPVTNALAPEALEPVDRLL
                     VARAALLVAECRKAYDRFEFHTVARRLREFATTDLSSLWCDVRKDALFVLGKDDPARR
                     SAQTAAYRLAEALALLLHPICPFTAEEIWEALPGNEGGTPALATWDDLRLATLSDEAH
                     AAWGRLLDVRAAFVAALEPLRRDGVVGTSAQARAEIERTPALEADLAVVGLDEARFAE
                     LLIVPEVVFTPTAGGTPLPVVSAQAAEGTKCPRCWQVRRDGDASGLCARCRRILAAAS
                     A"
     gene            60719..61228
                     /gene="lspA"
                     /locus_tag="PLP50_02920"
     CDS             60719..61228
                     /gene="lspA"
                     /locus_tag="PLP50_02920"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPA50532.1"
                     /translation="MSPVRLRALYLALAAFVVLLDQATKKMVQAWVPLYDRIVVIPGF
                     FDITHVENTGAAFGLFAGATSPYRPLLLNTVAFAVFLFVLVYAFRSPVHWKRLQLALA
                     GILGGAIGNLIDRVGAGAVTDFLRVHAADRWEWPSFNVADSAITVGVVLLALDIWKNP
                     ESEETKPES"
     gene            61228..62016
                     /gene="lgt"
                     /locus_tag="PLP50_02925"
     CDS             61228..62016
                     /gene="lgt"
                     /locus_tag="PLP50_02925"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPA50533.1"
                     /translation="MHPELLHLGPIVLPSYGAALGLAFVLGILLLRRRAPEYGIDRET
                     AVDMGIWLILSGLLGAKLLLLVVEGPAYYLGSFRGLLELFRAGGVFYGGLLGALVAAV
                     FFVKVKKVPFLAFADAAAPAVALGQAIGRIGCLAAGCCWGGQCTKPWAITFSDPKAGE
                     NVGVPIGVPLHPTQIYEALGLLALVGLVLWFGKGRVPGRTFSVYLVGAAILRFVVELF
                     RGDPRGTVPGTDLSTSQGIAIALFVLGVGIVLAGRKKADAPAEA"
     gene            62013..63011
                     /locus_tag="PLP50_02930"
     CDS             62013..63011
                     /locus_tag="PLP50_02930"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPA50534.1"
                     /translation="MSGDGERPRRSFTADRGDDRERLDRVLLRHLADLPEASRTKVQE
                     WIEAGLVLVNGRPVTKTAAKVAAGDAVDTVLPPPPPPKPELIPQEIPLSILFEDDHLL
                     VLDKPPGLVVHPAVGHRDGTLVNALLFHSKDWAGEADRPGLVHRLDKDTSGVLVVAKT
                     EAAHSGMAKAMKARTLVKEYLAVVYGRTAVAKGKVDLKILRDPHDRKRMTTSKTEGRE
                     STTLYERLAESTGERAGLSLLLCTLVTGRTHQIRVHLKAIQHPIVGDATYGSPRWKGI
                     ADPALQAACRDFPRQALHARRLAFDHPVTRARVDTIAPIPSDIAALLEAAGLSAPA"
     gene            complement(63012..64223)
                     /locus_tag="PLP50_02935"
     CDS             complement(63012..64223)
                     /locus_tag="PLP50_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA50535.1"
                     /translation="MSKHDVVVVGGGISGLTFAAEAARAGRKVLVLEQEDRAGGCLAT
                     RRAGSGYWFELGAHTCYDSYVGFTRVLEACGLRDSVVGRAKTHLRFLDGDRLVPGSNL
                     GVLLRLFSWPEAAVSLPRLIGAKKDGRTVAEFYGRIVGKRNYARVLGPMLSAVPSQSA
                     DGFPAGMLFKMRDTRRQDFPRSFTLAGGLQTAAEALATTPNVTLTTGMAATSVAAIGD
                     GWVVTSSDGACHEARIVAVATPPGAAAAFLGGATPELAGAAAAVKEAVVETLGFAVRS
                     AKVSRIPVSMFLVPKADSFHSIVTRDSVPDAEWRAFSFHFKPGLAANERLERAARVLG
                     LSRADLEEVAEKRTVLPSPALGHEAIVAEIDRLSAGKRLCVLGNWFAGLSIEDCVDRS
                     RTEWARVAALG"
     gene            64371..65216
                     /locus_tag="PLP50_02940"
     CDS             64371..65216
                     /locus_tag="PLP50_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50536.1"
                     /translation="MKNVLARTAIAASVFLCLGSPRSAFAQDDPQVLEGEAAFKHPST
                     QVVLKAAEHFRAGKIDAGMALYTASEQADWKKSSEKAELSARRKERAPDPKAFAEGIR
                     KGGTLTLYATEGQLRAPLPNRAMGIAYLALENGTWRLTGGPMAIENAQDPAKEERFHG
                     DEMRAHPVYALALKYAEMLHAKKDDSFMQLATKRAREKWAAEPASERSESAAYRRRTI
                     PKKADLAASLKDGGFIVVEDDVRASLVLVSVTQSSPEPGTVSSTSSTLSLGFELEDGE
                     WRVAN"
     gene            complement(65323..66258)
                     /locus_tag="PLP50_02945"
     CDS             complement(65323..66258)
                     /locus_tag="PLP50_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPA50537.1"
                     /translation="MRRHLVTALCLMLLAPAALAQKAPKDVSELVARLASSSGSERAS
                     AARSIGYQKDAALPAVPRLLELLAGDRDNEVRVSAAEALGNIGPKCAKPAVPALMKAA
                     KEDKWPKVRSASLSALGEMREAAKPAIPLLRESLRDPDGFIAQAARNALFRVQPDAKD
                     EVVAIEDATRPKQKGSLFDDLSQLQAVLPGRAPEVYELAIYPGFALATTACAEAGSGR
                     CQLKYEGGAVTGPNDGSEDDCEKKIALAKVDFSVVPGLVKQAPALLGAPGGAVTVVQL
                     SAGVFCKSYGWIVHVKDGGMVQFKLNGKVDKVTKF"
     gene            66636..67031
                     /locus_tag="PLP50_02950"
     CDS             66636..67031
                     /locus_tag="PLP50_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50538.1"
                     /translation="MKSSLCKVFSLAALVLLAAGSAVADDAAVFRLDQAVQAGTLTLP
                     AGVYEFRVSDRGVVSVFDDETSAIVGVTIARRDPLDLAHRSASATLTHDRAVRSISIG
                     DFRYSFRAAPAPAGMAALQRPTTVVALAR"
     gene            67128..68384
                     /locus_tag="PLP50_02955"
     CDS             67128..68384
                     /locus_tag="PLP50_02955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPA50539.1"
                     /translation="MPRAPLPRVVVHLDMDAFYASVEVREDPSLRGRPLIIGHRGRRG
                     VVSTCSYEARRFGVRSAMPSVTAERLCPDAVWLPGRMSLYVDVSRRIREVLDGFSPLV
                     EPLSIDEAFLDLTGIAHDLADGRRIAAEIKREIRDRERLTGSAGVAPNKFLAKVASDL
                     EKPDGLVVIPLEEVPTRLWPLPVERLWGVGPKTAERLREQGLVRIVDLVNAPSGRLAR
                     ILGEGSAEHLLALAQGRDDRAVQPGREAKSISEERTYGEDLTRPADIDKALLARADGV
                     ARELRHEGLVARTVHLKVRTGDFETVTRAKTLSEPTDLAEVVLEAARELFRERIALKG
                     RGIRLLGVGVSGLEAPGVGQPSLFSDERQEKLRALARTADEIREKFGKAALARARLLA
                     TPNTTGTDADGSEDDAAEASSLPSVD"
     gene            68504..69283
                     /locus_tag="PLP50_02960"
     CDS             68504..69283
                     /locus_tag="PLP50_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50540.1"
                     /translation="MANVYVRAEGRPERPGRAPPRLPHGSARKRGIRRAPLPGLPPRR
                     PGAGRVGRALPDPACRIPFRSPKWATWLSALVPGVGDIYLRHFLLGSLEFVGSIAVLC
                     FALFAIVAAVATRDSEVLLVAAVLGVLFILLPRLFDFFLTLHMGRKGIVPLSLTPAPP
                     GVEDGSPIGPSRGASLPAFPTWTWLLFAAGALAVGATASPSLAEARVLEACRLAETGK
                     LAEARKLYEASAAAKPVPLPTAGPSPSLSGKGAISTAETSS"
BASE COUNT         9588 a        25727 c        24759 g         9290 t
ORIGIN      
        1 ggccggactg gctccggggg ggcttgccat ccgggcgccc ccccggcacc ccccccacct
       61 tcgatctgac gtaagtgacc cgggccggac tggctccggg ggggcttgcc atccgggcgc
      121 ccccccggca cccccccacc ttcgatctga cgtaagtgac ccgggccgga ctgtaagccg
      181 gattctgtga ccggtcggct cgcgccttcc ggccggcgat cattcctctg ggcccgcgcc
      241 tctcggcggg gctcgagcgg cctacccgga aacgtcgcct ttcgacgcgg gggcgagccc
      301 cccttccgtt cccctattcg gccttgctcc gcgtggggtt tggcctgccg accccgttgc
      361 cggggttcgc ggtgcgctct taccgcacct tttcaccctt accacggccc ctctcgggaa
      421 ccgaggcggt ctgttttctg tgccactttc cttcgggtcg ccccgaccgg gatttccccg
      481 gcacgctgct cgctggagtc cggactttcc tcccctcgtt cggcgaaccg tccgagcggc
      541 gatcgcccgt ccggcccggg tcggcgagga ggatacgcca ccgccgccgc gcggcgtcag
      601 gcctccgtac ggggctttcg gcgggacgtg aagaggttga cgcccttcgc gagcttcggc
      661 acgcacagga ggaagaggcc tccgacggtc accgcgagga cgagggcgac gaggggcagg
      721 aagaccgaca cgatcgcctg caggacggcg aggacgtctt ccgcgaggga aagggcgaag
      781 ttggcgacac cggccgtcag ggcggtggac gtgagccgtg cggtcccctt gacgacgtgg
      841 gcggcgagcg cgatcacccc gccgcctccg cccgcgagga ccgtcagcga ggcttcggcc
      901 cccggcgcga cggcggcgac ggcgaggagg gccccggcca ccggccggac gaccgtgtgg
      961 acgatgtccc agacgtggtc caggccgggg atcttgtcgg cgaagaactc gaggacgtag
     1021 agcgcgagga ggacggccag ggtggccggc tccgccagga accgggcgat cgcgccggga
     1081 aagtcttccg ggaagaggtg cgcgattccc ccgtagacga ggagcacggc ataggcattg
     1141 aggccggcgc ccgccgcgag ccccagcccg gtcccgaagt gcgcgagcgc ctccaccacc
     1201 gcgggaggat agcgcccgga ggggcgctca gcgaggcttc gtcgccgcga tctccgcgag
     1261 cgccttcgaa gccttccggt tcgtcgggtc gagcttgagg acctccttca gcgccttcac
     1321 ccggcccgtt tcgttccgct cggccccgag cgcgtccgcg tacgccagcc agacctcgac
     1381 ggaatccgga aggttctccg tcgcccggcg gagcgtctcc gtggcctgac gaatccactt
     1441 cgggttccgg gcctggacct tcgcgagaag cgtccagtac tccggcttct cggggtcgaa
     1501 ctcgaccgcc tgccggagca tctcgtaggc cgggaagaag tcctccatct cgatcagggt
     1561 ccgcgcccgc tggaagttcg ccttcgcgac ctgcttctgc acctcgcgcg tcggctcgcc
     1621 gtactggacc ggcgccgcca gccctccgct cgaccgcagc ctgtcgtact gctgccgcga
     1681 cgccgggctc gcgaacgacc gatcggcctc ccggaccttc gcgaagagcg attcgagcgc
     1741 ctcgccggcg tcggcgagcg tcccccgcag ggcgttgtcg gggtggaaga gcctcgcgcg
     1801 ctcgtggacc gcccgtcgga ggtccgggtc ggcggcgtcc tccgcgaccc cgagcagctc
     1861 gtaggcggtc gtccagtcga gccggcgata ggcgttccag accgttctcg tctgctgctc
     1921 gaggatctcc gcgagacggg cgctcggcgg cgttccggcc acctccaggt tcaggctctg
     1981 gaagccggtc ggagcgtgct cgtcgggcgg cttcttccgc tcgacgacgg aaacgaggcc
     2041 gacggacatc agccgctcga cgacccgcag gtcgaggcag acgtccgcgg ccctcttcgt
     2101 cccgtcgacg gcgtcgaggg cctccgcctc ctccgacgtg acgacggcgc actgatatcg
     2161 gaggaggaga tccggagaga ggacgagctt cgcctggggg tccacccggg cggcgacccc
     2221 cgcgacgggc gggcgcctgc ggagcccctc gaggacgagg ctcgccgtcg actggtcgaa
     2281 agccacggga aggtcgggcg ccgaatcgag aggaaccacg ctgaacgtgc cggcgctcca
     2341 ctggaacgtc gagtagagga ccatctccgc gagcgcgcgg acgcacgcgt cggcgacgta
     2401 gggcgcgacg gcgccggcct cgcgcagggc gctggccagt ccgcgtcccg gctcggcgag
     2461 cgcgcgctcg aacgcggcga agaggaccga ctcgctcgcg aggccgaacg tgcggaggag
     2521 gtcgcccacg tgctgcgcct cacggttcga ggaggccgag acgagccgtc ccctgcggaa
     2581 ccagaagctc ctctcctccc cgcggtgcga gacgaggacc tgcgcgtgcc cgcgcgagac
     2641 gtaggcgttc cggaggacct cggcgagctc cgtcttctcg agcgtgccgc ccgagagcga
     2701 gccggttccg ggcttcggcg tgtccatgtt cgggcgattc taacggcggt cgccgccgga
     2761 cgactcacgg aagcgctcga ttcgggcggc agccgtcttt ccggccgccg cggccagggc
     2821 ctcgggggag gcggcgagcg cggcttcgcc gttcccgaag acggccagga ggcgcttcac
     2881 cgaggccgga ccgagcccgg gaacggcgag gagggggctt tggagcgtcc tccgggagcg
     2941 ggcccgccgg tggtgagaga gcccgaaccg gtgagcttcg tcgcgggccc gctggagcag
     3001 cttcaggccg gcgttgcgtc ggggcagggt gacggggagc gagcggcccg ggacgaagag
     3061 ctcctcctcc ctcttcgcca ggccgaccgc gggaagctcg atcccgaccc ggtcgagcgc
     3121 cgcgagggcc gccgagagct gccccttgcc gccgtcgatg acgaggaggt cggggagggg
     3181 ctccccctcg gcgcgccggc gctcgtagcg ccgcgtcacg gcttcggcga tggaagcgaa
     3241 gtcgtcgggc acaggccgat cgatccggaa cgcccggtac tctcccttcg ccggtgcccc
     3301 gtcgacgaag acgacgcagg aggcatacgt gtcgctcccc tggaagtgcg agatgtcgaa
     3361 gcactcgatc cgggacgggc gacgatcgag gtccagcgcc ttcgagaggg cgagcgtcgc
     3421 cttctccccc gcttcgcgcg ccgtccggaa gcgtcgcacg tggctcgtcc gggcgttgtc
     3481 ccgcgcgatc ctcacgcggt cgaacgcggc gccccgcttc ggggtccgga tcgccacctt
     3541 cgtcccgcgc cggtccgaga gccaggcctc gagcggctcg agggcctcct ccgcgacctc
     3601 cagcggaagg tggacctccc ggggaaggaa cgtcgtcgcg tcgtagtact gcgagaggag
     3661 cgcgacccag aactcctccg gttcgacctc cccgagcccc tcccagaaga gctccctcga
     3721 gtcgaggacg gccccgccgc ggacgtggag gacggagacg gcgacgtttc ctccgtcggc
     3781 gaagtccccg aagacgtcga cgtcctcccc ggcgagcgac tggacacgct gcttctgcgc
     3841 cagctcctcg accgtccgga ggcagtcccg gtaggcggcc gccatttcgt actgctcggc
     3901 ctccgccgcg gcctccatct tcgcggcgag gcgcggcgtc agctcctcgt tccgtcccga
     3961 gaggaagagg acgacgtccc ggacggcgtc gtcgtacgcc gccttcgtcg tcagtcccgc
     4021 cacgcacggt ccgaggcagg cgtgcatgtc gtggtagaga caggggcgcg gcagcgtgcc
     4081 gtcgatctcg atccggcagg tccggatctg gaacatgcgc tgcaccatct tcatcgcccc
     4141 gcggcaggtg cgcgccggga ggaacgggcc gaagtagctg tgcccgtcct tcaggacccg
     4201 ccgcgtgacg tgcgcgcgcg gccactcctc ccccgtcgtc accttgacgt acgggtaggt
     4261 cttgtcgtcc cgcagcagga tgttgtaccg gggcttgtgc ttcttgatga acgcgttctc
     4321 gagggcgagc gcctcgctct ccgtgttcgc gacgaccgtg tcgatcgtct cgaactccgc
     4381 gaggagcgcc tcggtcttcg ggtgcttcgg accggccgcg aagtacgacg cgaggcgctt
     4441 ccggatcgat cgcgccttgc cgacgtacag gatcttcccc ttcccgtcgc ggtagaggta
     4501 gatcccgggc tggtccggat agacggagag atcgggcggc cggcccacgc gacgattcta
     4561 gagagggcgc aggagggaga gcgccctcag aggaccttcc cgaggttcga cgcgatcgtg
     4621 gagaccttca cgtagccgaa gaaccggccg ctcgcgagga ggccgaggcc gtccgcgacc
     4681 ctcgtccgcg cgtcgcgcgc gtagaggacg ccgaacgcct tccgcatgaa ctgctggaac
     4741 gctccgtcgt acgggtacca ccagggggcc gacggcgagg aatcgaggtc gacgctgacg
     4801 tgcttgacgt tcacgagctc ccggaggccg tggacaccgt ggctccgtcc gatcccggac
     4861 ttcccgaggc cgccccagga ggcccggggc tcgccgaacg agaagaggtg gtcgttgatc
     4921 gtcaccgtcc cggcccggag ctcctcgacg agcttccgcg ccgttcgcgt cgatcgcgtc
     4981 cagccggagg cggtcagagc gaactccgag tcgttcgcga gggcgatcgc ctcgtcgagg
     5041 gagtcgacga ccgcgatcgg aaggaccggt ccgaacgtct cctccctcat cacccgcatc
     5101 ccgtggtgga cgtcgacgag gacggtcggc gggaaccagt tccccttcgc ggccggccgc
     5161 tcgccgccgg tgagggcccg ggcaccctgc gcgacggcct cgcgcacctg cgtgtcgacg
     5221 aagtccctct gctcggcggt cgtcatcggc ccgaggtcgg tcgcctcggc gagggggtcg
     5281 ccttgccgga gacgccgcgt cttctcgacg acccgggaga ccagcccgtc ggcgacctct
     5341 cgttcgacgt agacgcgctc gatcgagccg cacgcctgac cggcgttcgc gaaggcgccc
     5401 cagacgaggc cgttcgcggt ccgctcgagg tcggcgtcgg ccgcgacgac ggcggcgtcc
     5461 tttccgccca gctcgaggac gatcggggtg acgttccccg cggccttgcg catcacgtgc
     5521 tgcccggtct cgacggagcc ggtgaagagg accttcgcga tccccgggtg ctccagcagc
     5581 cggtcgacgt cggcgccggc agccgggacg acgttgacga ccccgggagg cagccccgcc
     5641 atccggcaaa gctctccgat ggccagaccc gtccgggccg tcgccgaggc gggcttgagg
     5701 acgacggtgt tgccggcagc gaggcaggcc gccacctcca ccatcgggat cgcgaacggg
     5761 aagttccacg gagtgatcac cgcgatgacg ccgaggggct cgtagcggtc ggacgcggcc
     5821 ttgtgggcga agaggagctg ctcgtagtcg accggccgct cggcgaggag ctcatcggcg
     5881 tgacgcgcca ggaaccgcag ggcgtcggcc gccgggacca ggtcgagggc gcgcgcctcg
     5941 gcgaccggct tcccctgctc ggtcgcgatg agccgcgcga gctcgtcgtg agagcgccgc
     6001 accgcctcga ggagggcctc gaggtgtttc cgccgctctt gatggctccg tgccccccag
     6061 tgaagaaagg ccttccgcgc cgcggcgacg gcggcgtcga tctcctccgg ccctccgcgc
     6121 cggacctcgt ccaggagggc cccggtggca gggtttctcg cgacgtacgc cctccccgtc
     6181 tcgacgtcga ggccgtcgat gatcggcccg gccggcgggg agagccgcct caaatcatcg
     6241 ctccgcgggt gatcttctcg acgtgcctgc gatagaaggc gacgaggttc tgcacgtagt
     6301 cggtgaaggg cgggcaggag acgccgaacc gcgcgaggtc ggtttcggcc tgggacgagt
     6361 cgtagcgggc cgggtggtcg aagtactcca cggtgacggc cggcatcccg aaccagtcct
     6421 ggatcggctt cggagtgaaa aggagcttcg cgagaaccgt cgggaccgga acgtagacga
     6481 actgcttgcc gagcgccttg gcgaagagct cctcgatctc gaacgcggag agcggacgcg
     6541 ggtcggtgag gtgataggtc ctcccgaccg atccctccca ggtcgagagt cgggcgatcg
     6601 cctcgaggac gaagtcgacg gggacgaggt tcaccgggtt cgttcccgtc ccgaccttca
     6661 cgaaggcgcc gggcgaagga agctggcgca tggcgttcag ggcgaagtag ggaccgtcga
     6721 acttcgccgt ctcgccggtg cgcgagtcgc cgacgacgat ggcggggcga taggtcgcgg
     6781 tcgggagggc gctccctttc accgcctcct cggccaggaa cttcgtctcc tcgtagtagt
     6841 tcttgaacga ctgccccacg tccaggtccg tctcgcggta gagcccgacg gccgtgcccg
     6901 acacgtaggc cgtggagacg tagtcgaagc ggtgcaggtg cgggcactct tcgaggaacc
     6961 cgaggacgtt ccgcgtccct tcgacgttga tccgcatgcc gacctcgcgc ggcaccgcga
     7021 ggtcgtagac ggccgcgagg tggtagcagc cggtcagctt gcggtggagg agccgggcct
     7081 ccgtctcccc gatcccgaga cctggcgcgg tgatgtcccc gacgacgagc tcgatccgcc
     7141 cggtcgtccc cgggtgcgac tcggccagct gcgagaccga ggagcgcgcc aggtcgagga
     7201 acttctcctg gacgaggcag tgaaagacgg tgtcgggctc gagctcgagg aggcgcggga
     7261 tgagccgcgc gccgatgaag cccgggaacc ctgtgaagag gacgactcgg gacggggcgg
     7321 ggttcgcgct cgctttcttc ctggccattc cgtgcctctc ttcccgacag tctagagcgg
     7381 tttctgcaat gcggcgtccg tccgcagtct ttcccggaac gcctcgatcg cccgcagcat
     7441 cgcgggcggc cgggcgcact cctcgccgtc caggaagagg atcggcgcgg cggtcgtgag
     7501 cgagccgagg aggaagccac cggcgaggag ctcgcgacgg ccgacgccgc ggcgctcccc
     7561 cgccccttcc aggaacgctt cggccgtgac cgacgggagc ccccccgggc cgagcccggc
     7621 gagccgccct ccgttccacg ccacgagagc cgtggagctc ccctccacat aggcgtcccc
     7681 ttcccggaag agcgcctcgt cgcagcccgc cttccgggcg tgccgaagcg cggcgacggc
     7741 cccgaagtag ctcgtcgact tgaatccgga ggagtcccgc cggaaacgct cgggcgcggt
     7801 caggacgcgc gagccctgcc ttcgcgcgag cgtgttcggc gggatcgcgc ggaccgacgc
     7861 gtcgaggcgc caggaggccg gctcctcgac gtcgtgaccg atcgcgatcc aggagaggcg
     7921 gagggcgcgc tcgtcgtcgc cgtcctggac accgccgagg gcagcccggg acgccgactc
     7981 gaccgcctcg gaagaggggg ccggcagctc gagcgccccg agcgtcgcca gcagccggcg
     8041 caggtgcgcc tcccagagga cggcgcgtcg gccgacgagc aggagcgtct cgaagaatcc
     8101 gaagccgcgc tcgacgacgg gcgcgtcgta agggatcggg tcggggctca tcgcacctcg
     8161 ctcgcgcccg ccgcggcggc gagcgcccgg aagaactcgc gggacttcgt ctccgactcc
     8221 tcccactcgc ggcgcgggtc cgagtcccag acgatcccgc ctcccgcatg gtaggcgacg
     8281 acgcccccgg tgacgatcgc ggtccggatc gccacgttca ggaccgccgt cccgtcgccg
     8341 cggacgaaac cggcggcgcc ggtgtagggt ccccgcgggg cgggctcgag cctgcggatg
     8401 aacccgagag ccgaccgctt cggcgccccc gtgatcgacc cggccgggag cgtcgcgaga
     8461 aggagaccgg agattccctc ctccggcgcg agcgatccgg cgaccgtcga ctcgaggtgg
     8521 tgcaggtggc ggtagctccg cacggcgcgc aggtcgggaa cgtggacgct ccccggcgtg
     8581 cagacccgac cgaggtcgtt gcggacgagg tcgacgatca tcaggtgctc cgcggcgtcc
     8641 ttctccgacg acaagaggag ggcgcgggcc gcctcctccg atccgccggc cgtcctcgcg
     8701 acggtcccct tgatcggggc cgaggcgacg ctccgggagc ggaggtcgac ctccaggaag
     8761 agctcgggcg agttcgagac gacggcgacg gcgcccgtgt cgacgaggat ggcgtaggga
     8821 acggggttgt ccgcgtcgag gagcgccgcg aggcggcgcg ccgacacggg aagagcgcgg
     8881 acgacgcggc gcgtcaggtt ggcctgatag acgtcgcccc tcgcgattcc cgcccggatc
     8941 tccgagacgg cggcggacca ctcctcaggc gtcatctcgg cggggggacc gaatggctcg
     9001 gccgggccag gccgtccgga aggacccggg cgatcggtcc gggcgccggc ggccgcccgc
     9061 aggtcgaaga gcccgaacca ggcgagaggc gtcggcggcg ggcggaacag ggccgtcgac
     9121 ccctcgagcg cgacgccggc ctcgtaagtc aggaatcccg cggcgacgag ctccggacgg
     9181 cccgaccgcg acaccgcgtc gagccgttcg agaagacgcg ggagcccttc cgggcgggcc
     9241 gcaggcacct cgaggacctc gagagggtcg gcgagctcga gcggtcccgg gagcttccac
     9301 ccgggaccgc gcaggaggac ggagggggac ggcacgctcg ggtagtctac ggagcgtgag
     9361 cgaagagcgc tgggtggaga ccggggtgaa ggtccgctat gccgagaccg acgcgcaggg
     9421 agtcgtctac cacggctcct acgtcgtctg gttcgaggtc gggcggacgg aatactgcga
     9481 gaccgccggc tacccctacc cgcggatgga ggccgaaggg gtccagatcg tcgtcgtcga
     9541 cctctcggca cgcttccggc ggcccgcccg gtacggcgac gtcgtgcggg tcgcgacccg
     9601 tctcgccgca gtccggagcc gcggctgtac gttcgcctac cgcgtcttcc tgccggacgg
     9661 cggcctcgcc gtcgagggcg agaccgtgca cctcttcgtc gacgccgcga ccggccgcac
     9721 gatcgccgcg cccgaaccga tccgctcggc tctcgccgcc ttcgcggcgg gctgaggcct
     9781 caggtcgcct tgccgttcgg cagcttcttc tggagcgagc ggtagacgag cgaaggaacg
     9841 agctccgaga cgttccctcc gagccggtgg acctccttga cgaggcggct cgagaggtac
     9901 gagagctcct ccttcggcgt caggaagacc gtctcgacct ccgggtcgag gcgccggttc
     9961 atgagagcca tctgaaactc gtactcgaag tcggagacga cccgcagccc acgaaggacg
    10021 atgttcgtct tctcggcgcg gaggaagtcg acgaggaggc cggagaacgc cttcactttc
    10081 accttcggct cccgccggaa gacgtctcgc gtcatctgca cgcgctcctc caccgtgaag
    10141 agcggggctt tttcggtgtt gcagaggacg gcgacgagga cgcggtcgaa gatgtggcag
    10201 ccccggcgga ccaggtcgac gtgcccgttc gtcatcgggt cgaacgagcc cggatagacg
    10261 gcgattcgca tcggaacgcg gattctagcg gctttcggtg agcgcctctc cggcttttcg
    10321 cgccgccagg acgagcagga agagggcggc ggcgggcggg acgacgcacc accaggcgct
    10381 cgtcaggctc tccctcgcgt ccgcgagcgc ccgcccccag gaggccgtcg gcggggggac
    10441 gccgaggccg aggaacgaga gcgacgcctc gaggaggacc gccccgccga cgacgtacgg
    10501 gagcgtggcg agggcggggg cgaccgcgcc cggaaggacg tggcggagga cgacgcgtcc
    10561 ggggctcgcg cccgccgccc aggccgcctc gacgtacggc gaggcggcga ggcgcagcgc
    10621 ttcggcccgg acgaggcgcg ccgcctcagg ccaggccgtc agcgcgatga ggagcgccgc
    10681 cgtgaaacgc gagggtggga cgagcgccgc gccggcggcg acgaggacga gggccgggaa
    10741 cgcctggacc acgccggtga ggacgaggac ggcccggtcg acgcccggac cgagcgtgcc
    10801 ggcggcggcg cccaggagcg ttcccgccgc gaacgcgagg gcggctgccg cgaggccgac
    10861 gaggacggag acgcgcgcgc cgtggaggag tcgcgagagg acgtcccttc cgaggtcgtc
    10921 ggtcccgagg aggtgggtgc cgtccggagg gcgcaggcgg gccgcgaggt ccgtcgcgtc
    10981 ggggtcgaac gggacgggcg tcagcagctg gtgagcgagc ggagcgtccg cgggccggtt
    11041 ccgtgcgagg acgggggcga ggagcgccag cgccgggaag agcgcgagga cgaggaggcg
    11101 tcgtcggacg caatggcgcg tcatccttcc tccacgtcga cgaggcgggg atcggcgaac
    11161 gccgcgaggg cgtcggcgag gagcgtcgcg aggacgacgg cgacgcccga ggccagcgtc
    11221 aggccgagga ccgtcgggag gtcacgggcg aagaccgaat cggccaggag ctgcccggtt
    11281 cccggaacgg cgaagacccg ttccacgagg acggagccgg cgacgagctg ggggacgagc
    11341 gacgcgagga gccccgcgaa cgccggcacg gaccggcgga gcgcccgaag cgtctcctct
    11401 cggggcgact ctccccgcgc gcgcccagcg atcgccgacg gagaacggag cgcggtccgg
    11461 acgctctcgc ggacgacgcg ggcgatcgga gcgagagcga ccagcgcgag cgtgagggcg
    11521 ggaagggcca gacccgcggg cccggcggag gggtccgcga agacggggac ccaccgcagc
    11581 ttcactgcga acaggaggag gaggaggaga ccgagcgcga agctcggaag cgaatagagg
    11641 gcgtcgagga ggagccccga gaaccggtcg gcccgccctc ccggccggcg tgcggccgcg
    11701 atgccgagcg gcacgccggc gagagccgcg aggaggagag cgacgccgtt cacagagagc
    11761 gtcgccggaa gcgtctcgcc gatccgttcc gtcaccttcc ggccgtcgcg aaaggaccgc
    11821 cccaggtcga gccgggccgc cgatccgagc cacgcgccga ggcgggcggc gaccggccgg
    11881 tcgagcccgt gtacggcgcg gaaagctcgc agcgcctcct cggacgccgg acgtcgtccc
    11941 gggaggcccc cggccgcgag acgggccggg tcgccgggag ccgccgagag gaggaggaag
    12001 accgcgacga ggacgccgaa gagcgtcccg accgcgccga gggcgcgccg cgccaggaac
    12061 cgcgtcacga gcgaggatgt tacgcggcgg cggccggccc gtccgtcaga tcggcttcgc
    12121 cccgcgtgcc ggctgccacg cggctcctcc cggccagaag agccagaggc cgatcggcga
    12181 gctcgttacg ttctcgatcc ggtcgcgcac gccccaggcg accgagaccg tatgcaggaa
    12241 cgtgaacggc tggtcctcgg cgatcacctt ctgcagctcc gcgtagagag agcgcgcctt
    12301 gtccctgtcg aacgtcgcgg cgatctcgtc catgagcgcg tcggcccgcg ggctccgata
    12361 gaacgcgtgg ttcattccgc tgggcggcac ctgagaggag tgccagttgg gccgcaggtc
    12421 ggggttcggg tcgaggttca gggagagcga gcaggcctcg tagtctcccg tgtcgacccg
    12481 ctccacgaac gcgccccact ccatcggcga gagcttcgcc tcgatccccg cctggccgaa
    12541 cgcccgctgc gcgacctcca tgacctgtcg ctgccggtcg ctggcgcctc cgaaagcgag
    12601 cgcaaacgcg aggcggtcct tccctttcgc ccggaggccg tccttcccct tcctccaccc
    12661 cgcctcgtcc aggagggccg cggcggcgtt cgggtcgtga gggagaggcc ggagcgtcgc
    12721 gtcgtggggc cagagaccgg gtggcagcgg gccgttcgcc ggcttggcga gcccggcgta
    12781 gagctcgcgc gcgatcgcct cgcggtcgac gagcatcgtc agggcgcggc ggacgcgggc
    12841 gtcggagaac tgaggaaggc ggttgttcca ggcgatgtag gtgtaggcga tctcgtcgta
    12901 gaccgtttcc cggatcgtcc ccgccttcgc cgcctccggt cgctgggcgg cgttcagccg
    12961 cgtctcgtcg agcgcccccg ccaggagacc ctggaacgca gcggacgact cggggacgac
    13021 ccggaggacg acctgctcgt acgcgggagc cgccccgaag tactgggtat tccggacgag
    13081 acggatcgtc ggcccgtccc acgaggcgag ccggaagggg cccgtcgcga gcggccggcg
    13141 gttcgccggg ttcgtcgcca cgtccgtccc ccggtagacg tgggccggga ggagcggcat
    13201 gttgaaggcg tcccgctggg ccgcgtaggc ctttcggaac gtggcgcggg ccgtccgctc
    13261 gtccaggacc tcgacccgct cgaggtcggc gaagaggctc cggcggtaga gcgccggcgt
    13321 cttcgggtcg aggagcgcct cgagcgtgac cttcacgtcc tcggccgaga tgggagtccc
    13381 gtcctcccag cgggcacccg agcgaaggcg gatcgtgtag acgaggcggc tctcgtccgc
    13441 ctccaccgac tcggcgaggc ccgggacgag ggtgaggtcc tcgtcgtagt cgaggaggtt
    13501 ccgctggaga agcgcgatga cgacgttctc ggggtccgac gtcgccgtga gcgcgttcag
    13561 cgtctgcggc tccccttcga gccgtcggtg caggacgagg ctctggtctt tgccgacggg
    13621 gccgccgcac gcggacagga cgagaagcga cgcgagcgcg agccgcttca ccggagctcc
    13681 ttcgggtact cgacggagat cgacgcgccg ttcccggccg cgtcgacgac gcggacggcg
    13741 aggaatgccg gacctccggg cttcgggacc ttcatcgtca gctcctcttc tcgcccgtcc
    13801 agtgtcccgt cggccggcgc gaggggccgc caccggtctg cggagacggt cccttccgcg
    13861 cggacgatcg gcgagagggc gtccacggct ttcaccctga ggacgacgaa gccgccctcc
    13921 tcccgcgcgt ccgatcggac gagcgatggc ggggtcccgt ccacgagcgc gacgtcgctc
    13981 tcctcgctcg tcgagagggc ctccccctcc acgttcgccg cacggtccga agcggtgacg
    14041 cggaagcggt accggccgtc gggaagcggc gccgtgtcga acgagaagaa ggcgtcctcg
    14101 aggtcgcgcc ggatcgggaa ccaggtcgcg gagccctccc ttcggacctc gaggtcgtag
    14161 cggagcgtgt ccccgttcgg gtcggtcccc ttccaggtga ccgtccggaa tcccttccgg
    14221 aagagcttct tgcccggcgt ctccgcgggc ttctcgggct cgaggccggc gaacgcgccg
    14281 ttctcgtcgg ggttcgtcac cgagaggacg gccggaccgg acgcggcgcc ggaacgtgcg
    14341 aagaccgctc ccggctcgag gacggagacg ttctcgacga tcggccgcgc atttcgctcg
    14401 gcccacgagg tctccacccg ctcgatgacg ggagcctcgc cgcgcggcga ggccgcgagc
    14461 tcgacgcgcc actggaagta gcgcgcggcc ggcggggccg cctcccccga gctcccggcg
    14521 cggacccagc cggaccaggt cccgtcgggc gtgccgctgt tcccggaacg aaccgagaga
    14581 agggccgaag cgcccttcgg caccgtcccc tcgagccgga tggttccgaa tcgcgcgagg
    14641 cgtcccgcgt cgcggatcgc ggagaggtac gtcccgcggc cggtcgcgcc cgccgggcgg
    14701 aggacggccg gtgagctcga cgccacgatc gcgaaacctc ccgggacggc gggtgaggcg
    14761 acgacgagcc gctcgccggc ctgcgcctcg aggcgcagct gccgcccctt cagcgagtag
    14821 acgcgcccct tcggcccggt cgcgaggagg agcgcgccgc gcccctcgtc ccagcgggcc
    14881 ccgtagaccg tttcctcggg gaggatccag gcgggctcga cgaacccgtc cgcggcgacg
    14941 aggacgacct ccgcgttccc ttcccgcccg gagggggctc cggcaggagg ccgcaccggc
    15001 gaggtcgtcg aggtgaccga gacgctcccc tgcggaacgg cctcgggcgc cccgcccggc
    15061 gccggtggcg gcgccgctcc ggctcgcgcg ggcgtctgcc ggggttcgct ccgctgctgg
    15121 gcgagcgtcg gcacggccac cgacgtcacg accgcgtaga gccccccctc cggcgccggc
    15181 gcgagggcca cgacctccgg ccgcccgaag tcgtggagcg tccgaacgct gccgtcaggg
    15241 gcgacggcga cgacgaggcc gcggtcggag gtcccggcca cgagagagcc atccgcgcgc
    15301 aggaggagcg accggacgtg cgtgtcctcg agctcggcgt gaagcgaggc cttgccgtcg
    15361 gcgcccgccc gccagatccg gcccttcgtg cccgtgccgg cccagacggt tccgtcgggg
    15421 ccgacggtca tcgcccagat cgccgcctct ccggtctccc cggcgaggga gccggccttc
    15481 gatgcgtcca tcgccttcga gtcgatccgc cagaggcggc ctcccggaga ggtgccgcag
    15541 acgacctcgc cccgcggtcc gacggcgacg gcggtgacgt tcggcgcgtc cgcctcgaag
    15601 aggaggcgca ccgagccgtc cggctcggag acgaagagcc gcccgagtcc gccccccgtc
    15661 gcgacgtaga cgcgacccgt ggcgtccgcc gccgctccga agacgaccgc acccgcagcc
    15721 tcctccggcc acgccggcgg cgagaagacg gggccgagcg tcaggcgccc gtccgaagtc
    15781 agcgtcgtcc cgtcgagctc gccggccagg aagtcacggg ccgacccgaa cgtcgagaga
    15841 cgcgtcgcgg cgaagagcgg cggtgccgcg ggaaggacga gggcgagcgc gaggctcgcg
    15901 gcgagagctc ggttcgaggt caggttcatc gggttcctcg cgagcggggc cgctcgacgt
    15961 cgacgtcgat gcggacggcg cccgagacgg ggcggtcgaa ggggacgatc tcctccgcga
    16021 cgagccgggc ttcgacgtcg gcgatcccgg tctcgccctg ctcgcgggca ccggcgagga
    16081 gggcgagcgc cgtcggcggc agggcctcga tcgtctcggt cctcgtcgcc gcgcctcgcg
    16141 tggcgacgag gaggccggcg aggagacggt tccccggaac gatccgggag acgaaggacg
    16201 cgagctgcgg cagcgttcgc ggctcggagg ggtcgagctg cattcggagc gcggtggcgt
    16261 tgctgccgtc cgcgacgacg agggtcgccc ttccttcggg cgtccccgcc ggaactcgaa
    16321 gccggacgac tcggacgctc tcctccccgc ggcggtccgc gagccggatc gcgaccgaga
    16381 gcgtccctcc cggggcgacc ttcctcgtgc ccggggccgc atcgacgagg cgcagcctct
    16441 gctcgtcgga cgcgctcgtg aagcggagcc ggacggcgcg gacgcccggg tcctcgaggt
    16501 cgttgtcggc gaccgccgcc gcgagggcga ccgtcgtcag gaccgcgacc tccttcgccc
    16561 gccctcccgt cgccacgtcc tcgtaggcga tctccccggc cgccgtgtcg agcgcgatgc
    16621 gaaaggcgag ccggcgctcg cgggcggtcg ggtctgcggc cgtcatcgag gcgtcggcga
    16681 cgaggcccgc gagggtcggg aggagcttcg gatgcgcggc cgcctcgaat cggaagtccc
    16741 gcgccgggcc gccgccggcc tcgacccgga ccgagaccgg gaccgtctgc gcgggccggt
    16801 ccgtccgtcc accgacgccg gtgtcgcggt cgttcgtcag ccggtagagg gcttccccgc
    16861 cgttcgcgag cttgaaggac tggaagaggg acgacacgac ggtcacgacc tcggccggcg
    16921 cgaccggcag ctccacctcg ccggagccga ggaacgggtg gccgaaggcg acgaaccgtc
    16981 cgtccgggaa gacctccgtg acggtccccg tcgccccgag gctcaggtct cctcggacga
    17041 ggtaggcggt gacggctccc ccgggcacga gacgccgggg ctcgccggac ggggcgtccg
    17101 acgggagggg cgcatctccc gtcccgagga gcgcgaggtt cgcctcgctc gcgccgaggc
    17161 gggagagggc gtcgcggaac gactccacgg tcgacgccgg gactcccgag gcctggaagg
    17221 agagaagtgc agcgccgccc cgcccggtcg cggacgtcgg cagcgagccg aaagccgccc
    17281 ggcgaagctc cgcgacccga tccgcgtccg acgagctggc cagccgcgcc agaaacgtcg
    17341 cgcggtcggc gagcggcggc ccggacaccg ccccggccgc cacgcgggcg ggcgtctccg
    17401 ggacgatcga cagcatcgcc tcgatcggcg tcacccccgc gatcgcgcgc ttcgagaagc
    17461 cccagccgga ggcgatcgcg ccggccagct tcccgtccag gtagaccggg ctgccgctca
    17521 tcccggccac gacgcccgaa tcctcgagcc cgaggccgga gagcgagacg acgatgacgg
    17581 accgcgccgg ggccgggttc cggacgaccc cgacgacctc gacctcgaac cgctccacgt
    17641 cacggccccg gacgaccgtg agcccgtgcc ccttcatgcc ggttcggacg ccctccagcg
    17701 ggaggatctc cgccgaccgg gcgggcatgc cgaggagggc cgtcgcgagc gtgacgaccc
    17761 acacgtaacg ctttgccccc cttcgagatt ccgtgctacc ttgccccgga tttcggcccg
    17821 gtcggggcag gagcgacatg acggataccg gcggcgccaa catcgttcag ctgttcctcg
    17881 cggcggggtg gacggccaag gccgtgcttc tgatcctcgc cctcttctcc ttcgggtcgt
    17941 gggcggtcat cgtcggaaag ctctggagtt tctcacgggc cgacgcccac tccgggcggt
    18001 tcgtggcgca cttccggaag agccgcaagt tcgcagacgt cctcggcgtc tgcgaacagt
    18061 acccgtcgag tccgctcgtc ggcctcttcc gcgccgggta cgccgagctg gacgcccagg
    18121 tcaaggcggg gcgggacgtc gccccggccg attcgggccg gctcttcgtc aagagccttc
    18181 ccgccatcga acgtgcgctc gagcgggcgg cgggcgtcga gtcgaaccgg ttgacccgga
    18241 ggctcccttt cctcgcgacg acggcctcgg cgaccccgtt catcggtctc ttcgggaccg
    18301 tctgggggat cctcggcgcg ttccggcaga tcggcgtcac cggctcgacc tccatcgtca
    18361 ccgtggcccc cggaatcgcc gaggcgctgg tcaacacggc cgccggcctc gtcgccgcgg
    18421 tgcctgcgct catcgcctac aactacttca acggccgcgt gaaggtgacc cgttcggaga
    18481 tgcgggactt cgcgctcgag ttcctcaacc tggccgagcg ccacttcacc tgaggcgccg
    18541 gaaggagcgc ggatggccgt caagatcgac gacgacctcg aggcctactc cgacatcaac
    18601 gtcacccccc tggtcgacgt gatgctcgtc ctgctcatca tcttcatggt gacggcgccg
    18661 ctcctccacc agggcgtcga ggtgaagctc ccgaagtcgg tcgcccagaa cctcccgaaa
    18721 acgcccgagg accccctcat cctctcgatc acgaggaagg aggtctacta cctcaacgag
    18781 acgccgatcc cgaagcagca gctgcgggag aggctgaccc tcatcctgaa gaaccggaag
    18841 gaccgggcgg tctatctcaa ggccgaccgg aacctgccgt acggcgtcgt cgtcgagacg
    18901 atggacgtcc tgaaccgcct cggggtggag agcctcggca tggtcacgga gctccgaagc
    18961 gagaagggcc cgtgacggtc tcggaggtcc tggaagaacg gaaccggcgg cccgacgcgc
    19021 cgcacggcct cgcggtcgcg ctctccctcg cggcgcacgc ggcgttcgtc gtctttctgg
    19081 tcctcatctc gcagccccgg aagctgacga ccccgcccct catgtccctc ccggtgcgcg
    19141 tcgtctcccc cggcgccctc gcccggctct ccggttcgcc gggacgcccg gcggcccgtc
    19201 cgcagaccgc ggcgccccgc aggagagtca tcgagaagat ccgcgacgag gccccggccc
    19261 cgtccgcgga ggcgctcccg ctccccggaa agccgaagaa gggcgaggcg ccgaagccgg
    19321 ccccgcgcgc gtccgccccc gggccccggc cgaccgcgag cgactcggcg gtcgagctcc
    19381 cgtccgccgg gaacgggggc ctcggaggga gcggcagccc cggactctcg agcttcggcg
    19441 cctcgctctc cggcttcgac tccgatttcc cgttctccta ctacgccgaa cagatccagg
    19501 ccctcatcgg agccaactgg ctcaagccgg acgtcgccga ggggaccgcc tgcgtcgtca
    19561 cgttccgaat ccagcgctcc ggccaggtca ccgacgtgaa ggtcgagacc ccttcgggcc
    19621 tcgccttcta cgaccgtgcg gccgcgcgcg ccatctattc cgccaacccg ctcccgccgc
    19681 tcccgcccga gttccggggc gagcagctgg gggtccacat ccgcttccag tgaagccctc
    19741 ccggaggtcc gcgatgcgtc tcctttcctt cgccacgggc ctcgtgctcg tcgtcgccct
    19801 cgccccgatc gccgggcagc agccggcccc gaccgtcgcg ccgccggcgc agccgaccga
    19861 catctccctg gtcctttccc aggaaggagg acggcgggtc gctctcgcga ttccgtcccc
    19921 gaaggccccc gtgtcggacg tcgtcgagcg agacctcgtc gagcccttcc gctcggcgct
    19981 gacgggcgat ctcggcagct ccccctggtt cgtcctcgcg gaccccgccc tccacccgaa
    20041 gggcttccgc ccaccggcga cgcgcgaaga gggggacgcc tggatcgcct ccggcgccca
    20101 gttcctcctc gacacgcaga tccaggccga ggggacgaac gtcgtcgtga cggcccagct
    20161 catcgacctg cggaccctcc gcccgatcct cggcaagcgg tactcggccg cgggcgtctc
    20221 gatacgccgg gtggcgcaca ccatcgcgaa cgacgtcgtc cgccagttca cggggcgtcc
    20281 cgggccgttc ctgacacgaa tcgcgttcgt ctccgaccgg gacggcggac gcggcaagga
    20341 gatctacgtg atggactggg acggcgagaa cgagcggcgg ctgacgttcc accgctcgct
    20401 ctcgatcgcg cccgactggt cgtacgacgg gtcgaagatc gtctaccagt cgttcgtgag
    20461 cacgcctccc ggcctctact ggttctccct gtcgggcggg acgagaaaca aggtcccggt
    20521 cccgacggag ctgaactcgg cgccctcgtt ctctccggac gggaagatgg tcgcctacgc
    20581 cgggagcgtc cgcgggaacc cggagatctt cgtcgtgaac ctcgacggct cgggccacag
    20641 gaggctgacg aactcctcgg cgatcgactc gagcccccgc ttctctccga acggccggga
    20701 gatcgccttc acctcgaacc ggcaggggac gccccagatc tacctcatgg acctggaagg
    20761 ggcgaacgtg aggaagctga ccctgaccgg aaactggagc gacgagcccg ccttctcgcc
    20821 ggacggaggt cgcctcgcct acgcctgccg caacgagggc gacttccaga tctgcgttct
    20881 cgacctgacg acgggccgga ccttccagat ctcgagcggg cccggaacga acgagaaccc
    20941 cacctggtcg ccggacggct cccggatcgc ctgggagctc cagcgtggcc gttccaccca
    21001 gatcgtctcg gctggaatcg acggctcggg gttcaaggtc ctcaccgagg ccggcaacaa
    21061 cggctatcct gtgtgggaac ggaccatgga ctgacgcggc cgcccgggtt gtccgggcag
    21121 gcggcagcgc ttagaatcga gatcaactga aagggcgtcg cgccttttcc caatcggagg
    21181 ttcaccgtga tgacgaagcc gttcaggtct ctgggactgg ttctcgccgc cggccttctc
    21241 gtaactgcgg gctgctgtac caagaagcct gcgccggccc cggccccgga agtgaaggtc
    21301 gaaccgactc cgacccccgt acccgtcccg gtcgaggagc cgatcgccgc gcccgtcgcg
    21361 gaaccggtgg ccgaatcgct tcccgccgac ctcgaggagc tcaacaagaa gggctatctc
    21421 aaggacatct tctacgacta cgacctctac gacgtccggg ccgatcagcg ggacgccatg
    21481 gccgcgaacg ccgagtggct gaagaagtgg cccaccgtaa aggtccaggt cgagggccac
    21541 tgcgacgagc ggggctccaa caagtacaac atggcgctgg gagacaagcg cgcgaacgcc
    21601 gcgcgcgact acctcgtctc gctcggaatc gacgcgagcc ggatcacgac gctctcctac
    21661 ggcgaggagc ggcccttcgt cgaggggcag accgaggacg cctggtccca gaaccggcgc
    21721 gcccacttcg tcgttaccgc gaagtgacgg ctctccgcat cgaccgtctc cgccggagcg
    21781 cccccctcgc gggggcgctc ctcttcgcgt ccgccctggg cggctgcgtc tccgggagcg
    21841 acatcgaggg cctgcacagg cacgtcaccg acgtcgagaa gcaggtcgac aagctctccc
    21901 agcagaccag ctcgaaggac gaggtcgaga agctcaacca gaacctgacg aagcaggtcg
    21961 ccacgatcct gcgctcgaac gccgagacgg ggacccgctt cgacgagctg acccgcgagc
    22021 tccagtcgct cgccggcaag ctggaagact cgaaccgccg gctcgcgcag ctctcgcagc
    22081 agctcgccga gacccaggcg aagctggatg ccggctctcc ggcgactctg accgtgccgg
    22141 tcgcgggctt tcatccgggc gctggcgccc ctccggctgc cgtcgtcccg gcggctccca
    22201 ccggcacccc ggcgccgaag cctccggccg gacgggccgg tccggccgac ctctataacg
    22261 ccgcgctcgg cgactaccag cgcgggcgct tcgacctcgc gcggcagggc ttcgaggagt
    22321 acgtggagac gtacccgcgg accgacctgt ccgacgacgc cctcttctgg gctgcggagt
    22381 gctggacggc gcagaagaag caccgcgaag cgctcggttc cctcgagcgg ctcctgaagg
    22441 agtacccgca gagcgacaag gcctcctcgg cgcaccttcg gaaggggctc gctcacctgg
    22501 agctcgggga gaaggcgcag gccatcgtcc agctccagtt cgtcgtccac gagtacccga
    22561 cctcggacga ggcgaagagc gcgcgccaga ggctgcgctc cctcggctcc gactcccgct
    22621 gaaccctcga taatcctccg cccgggcgga gggaagaaga cccgggaccg acccgaaagg
    22681 aaacgatggc caacacgaag caggcgaaga agcgtgccgc ccagaacgac aagcagaagg
    22741 cgcggaaccg cgcggtgaag agcgagaccc gacgcgtggt caaggcgacc cgcgccgcgg
    22801 cccccgagaa ggcgaaggag atgctgccga aggccgcctc ctcgctcgac gtcgccgccc
    22861 ggaaggggac gatccacaag aacaaggccg cgcgcctgaa gtcgcggctc gccaccggcg
    22921 ccaacaagaa ggccgccaag gcggactgac cgagctccgc gcgtcgcgcg gagcccacgg
    22981 gccgggtagg ccggaaacga ccccgtccgg ggctcgggtc cggcacccgg ctcctctcgt
    23041 acccggctcg ctcccgaggc gccgcggacc gcgccgggac ggcctaccga cggcggcgtc
    23101 agaacccttc gaggatcgtc gtgacgaggc tccgtgcgaa ggggcgcgcc atcgcctcga
    23161 tcgcggcgtt ctccacgtcg acgtagctcg aactcgtcgc cgcgaccggg tacggctgcc
    23221 ggaacaggaa cgacgggttc tcgaagagcg ccttctccgt cttccggtcg gtcagcttca
    23281 ggcgggtcac gacggcgatc tcgtactcga gcgcccgtcc ctggtcgtcg aagcggacgg
    23341 ggttgacctg gtacgagacg atgcgtcccg agagctcgac gtcggccgcg gaccggtccg
    23401 tcacgggcga gagccgggcg cggcccgcca gctcgcggag gacggcgtcg gtgaggcgct
    23461 gctcgagccc gacgcgggtc gtgtcgttca cgaacgtctc cacgaagacg gcgctcgctc
    23521 cttccgggag gatgccccgg ccggtcccga cgagggagta gccgcagccg gcgagcgcgg
    23581 ccgccagcgc ggccgcgacg aacgcgcgcc gcctcacttc accacgacgt tcagaagccg
    23641 gtcggggacg aagacgacct tcacccgctc gcgtccctcc agccacttcg ccacgccgtc
    23701 gagcgcctcc gccgcggcga gcacctcctc gcgcgaagcc ccgcgcggca ggacgacctg
    23761 gccgcgcagc ttcccgctca cctggacggc gatcgtcacc gactcctcca cggcaagcgc
    23821 cgggtcgaag gcgggccagc ccgagacgag gaggctccgg gtctccccca tccgctcgtt
    23881 ccactcctcg gtcaggtgcg gcgcgatcgg gtggagaagc gccagcagcg ccaggatcga
    23941 ggcgcgcgtc tcgcccgggt cggccttcgc gtcgccgacg agccccgcga cgtggtgcga
    24001 gaactccatc aggtgcgcga cggccgtgtg gaacgagaac gtctcgaagt cgtgcgtcac
    24061 cgacttcacc gcgatgtggc gtttccgggc cgccggcgag tccgccgcgg ccggggcgtg
    24121 cggcgtcgtg ccgtgcgcct tgagccagct ctcgacgagc gccttcacgc ggaagaggaa
    24181 ccgctcaggt ccggcgatct ggtcgtccgt ccactcggcg tccgactcgg gcggaccgag
    24241 gaagaggacg tacgcgcgga ccgcgtcggc tcccttcgcc gcgatgagcg ggtcgaggga
    24301 gacggcgttt ccgcgggact tcgacatctt ctcgacgcgc ccgccgggcg acatcctcgt
    24361 gatcattccc tggttgaaga gggccgagaa cggctcctcg aacgcgacga ggccgaggtc
    24421 cttcatcacg cgcgcgaaga agcgcgcgta gaggaggtgg aggatcgcgt gctcgatccc
    24481 gccgatgtac tggttcacgg gcgcccagcg ggcgatgacc gcggggtcga agggagcacg
    24541 gtcgttcgtc gggtcgaggt accggaggta gtaccaggac gagtcgacga acgtgtccat
    24601 cgtgtccgtc tcgcggcggg cctctccgcc gcacttcggg cacggcgtct tcaggaacgc
    24661 ctcgaccttc tcgagcgggt tgccgcccct gccggtgaac ggggcgtcct tcgggaggac
    24721 gacggggagc tggtcgtcgg ggacgggaac ccagccgcag gccgggcact tcaccatcgg
    24781 gatcggcgtc ccccagtagc gctggcgcga gatgagccag tcgcggagcc ggtagcggac
    24841 gcgggccccg ccgatgccgc gcttcgtcgc ctcggccgcg atcgcctcac ggcccgctcc
    24901 cgacgagagc ccgtcgtacg ggccggaggc cgtcatcgtc ccctcgccag accagcaggc
    24961 aatcgaggcg tcggccccct caggtcgccg gatcacctcg cggatcggga gtccgtactt
    25021 cttcgcgaac tcgtggtcgc gctcatcgtg agccgggacg gccatgatcg cgcccgttcc
    25081 gtagccggcc aggacgaagt tcgcgatcca gatcggcatc tcctcgccgg tgaacgggtt
    25141 gatggcatac gccccggtga agcgcccgtc cttctcggcc ccttcggcct cgcgctggac
    25201 gcggtcctgg ctccggatcg tcttcaccca ctcctcgagc gcggcgcggt ccgggtgccc
    25261 ctcgatcagc cgcggcaccg cgggatgctc gggggcgagg atcatggccg tcgcgccgta
    25321 gacggtgtcg gggcgcgtcg tgaagacctc gagccgctcg ccgagcttcg gcacctcgaa
    25381 ctcgaggtcg gcgccgacgg accggccgat ccagttccgc tgcatcgtcc ggaccttctc
    25441 ggaccacttc ccgagctggt cgaggccggc gaggaggctc tcggcgtaat ccgtgatctt
    25501 caggaaccac tgctcgagct cgcgctgctc gacggcgctc ttgcagcgct cgcaggtccc
    25561 gccctcggcc tgctcgttcg cgaggaccgt ctgacaggag gggcaccagt tgacggtccc
    25621 cttcttgcgg tacgcgagcc ccctctcgag catccggagg aagagccact ggttccagcg
    25681 gtagtactcc ggctcgcagg tcgtgacttc cttcgtccag tcgtagagca cgccccacgc
    25741 cgcgaactgc tccttcatcg cggcgatgtt cgcgagcgtc gactctcgcg gcgggattcc
    25801 tctctggatc gcgaagttct ccgcggggag gccgaaggcg tcccacccca tcgggcagaa
    25861 gaccgtctcg cccttctgcc ggaggaatcg gtgcagcgcg tccgtcagaa agtaggtcct
    25921 cgcgtggccg acgtggagcc ggtcccccga agggtacggg agcatgacga ggaggtaggt
    25981 ccccttcttc gaagggtcgt gggtgtcgac gaatgcggcg cggtcggcgt cccagcgggc
    26041 ctgccacttc ttctcgaggg cggtgcgttc ttcgcgtgcg tccataaggg acgccgagtg
    26101 tagcaaccgg gacggccgac tacgccgcgg ctcccgctgc ctctccgagc cgccgctcga
    26161 tctcggcggc gaggcgggag agcgggaacc ggcgcgccgc cgggaccgcc cgggcggcgg
    26221 cgtcgggcat ccccgcgatc accgccgtct ccgccgactc gcagaggacc gtccctccgg
    26281 cgagcgcgat cgcgcggcag ccttcggcgc cgtcgtcgcc catgccggtc aggacgacgc
    26341 cgagaacgcg cctcccgaac gcggcggccg ccgaggcgaa gaggaggtcg gcggaggggc
    26401 accaccggac tgacggtccg tggggccgga cgacgaggac ggcgcgctcc cctgcgtgcg
    26461 acagctcgag atgagccccg cccggcgcga tccagacgac tccgggtgtc gcctcggcgc
    26521 cgtggatcgc ctcgcgcacg tcccaccctg tcccggaggc gagccgctgg gcgagggagc
    26581 gcgtgaaggg aggcggcata tgctgcgcga cgccgacgac ggcctcctgc ggggggagcc
    26641 ccgcgaagag ctcccgaagc gccggcggtc cgccggtcga ggcggcgacg acgagcgccg
    26701 gcgggacgcc gagcgcgacc ggaacccgcc gcgggcgggg cacggcgccg ctcggcggcg
    26761 gagcgccgac gcgcttcgag cgccggacga ggctctcgat cgagagctgc cggacgccgt
    26821 ccacgacctc ggcgagccgc ctcttccagc gtgcgaggct ctccggctgc gacccggcct
    26881 tcgcgatgac gtcgagggct cccgcttcca gcgcggccag ggcgctccgt tcctcctggt
    26941 cggccgagat gacgacgacg ggcaacggcc ggttcgccat gatccaccgg aggacggcgt
    27001 ttccgtccat tcccggcagg ccgaggtcca gcgtgacgag accgtagtcg gtcgtcagga
    27061 gtcggcggat cgcctcctcc ccgctgccga ccgcgtcgac ctcggtgatc gcgctggagg
    27121 cccgcagagc ctccacgatc cggagcctct ggtaggcgga gtcctcgacg accaggactc
    27181 tcacgacgtc accttcggta gacgacgtcg ttcgcgaggc ggacgagcgt cagggcggag
    27241 gaggcgacgg aaagcgcgtc gctcgcgccg aggaggatgt agcccccggt cttgaggcgg
    27301 gaggagagga cctcgacgac gcgggcccgc gcctcctcgt cgaggtagat gaggacgttc
    27361 cggcagagga tgacgtcgaa cctggtgccg aaggccggct cgtccaggat cgagcgcgtc
    27421 tcgaagagga cgggcgcggc gagcgggggc ttcaggcgga agagcccgcc gggagcggcc
    27481 tcgaaatgct cgcggcgccg ttccgcggag gtcgcgcgga gcgactgggg gccgtagacg
    27541 gcggctcgcg cccgccgtac gtgggccgga gagaggtcgg tcccgaggat ccggaccttg
    27601 cggccgggaa ggacgccctt ctcgtgaagg acgatcgaaa ggctgaacgc ctcctcgccc
    27661 gtcgcgcagc cggccgacca gacgtcgagg gccccgtcct gtccggccga aaggagcctc
    27721 tccggcacga tctcctccga gaacgccctg aggctggccg gatcccggaa aaagtacgtc
    27781 tccccgacca cgactgcgtc gaggagcctg ccgaacagcg gcgaatcctg gggctcgttc
    27841 cggaggcgcc cgtagagctg gtagaacgat ccggcgcctc gcgcttcggc agcgaggcgg
    27901 ctctcgaccc ggaccctctg cgaatcggag accacgaacc ccgttctccg tcgcaggagg
    27961 tcctggtaga ggcgaaagac cgactcggcc agctccagcg ggcggcccgt cacgtcgacc
    28021 tctggcttcg tcctgcccgg gccgctgtgg ccagggcgcg cgcccagcgc ctctccgcgc
    28081 gggccggcgc ctgcatccag gcgcgtcggc aggcctcggg agcgagccgc gcgagggcct
    28141 ccgccgcgtc gtccgcctcc gccgggatcg attcctgcga gaggacgagc gtcatcgggt
    28201 cgaccatcgc ctcgtccgcg aggccggacg cggcgacgag gagcgatcgc aggacggccc
    28261 gatccgactc gacgacgagc gccgtcgcca cggcgccgga gagggccgag cgggagtcgc
    28321 ccggcggccg tcgcgcgacc gtctcggccg cggcggcccg cacctccgct cgctcgtcgg
    28381 cgagagccgt ggcgagggcc tcggccgcga ccgcctcgtc ggccacggag agggcgcgga
    28441 ccgcggcgca ggcggtctcc gggtcctcgt gtccgagcgc cgcgacggcg ctccgggccg
    28501 cctccgggtc gccggtcttt tcgatctcct ccagcgcggc gatgcgcacc gccggctcct
    28561 gtacgagcat ctcctccacg aggcggggac gttcggcgcc tgcgagtgcg atcacggcgg
    28621 ctcccgcggc cgcggcggcg gccggatcgt ccaccaggaa gacgaggccg tcccgccagg
    28681 tgacgcccgg caaggcctcg ccgcgctcgg cccgaagggc gaggccgcgc agcgccgccg
    28741 cccgcacggt cgcgatctcg tcggagaggc aggccacgat ctcgtgcgag ccgatccggc
    28801 gtccccgggc ggccgcggtg acggccgcct cgcgcacggc cgggtcgggc gtcgagaggg
    28861 cgccgcggac cgccgcctcc acgtcgggac cgtccgtccc ggcgagggcg gcgagcgccg
    28921 caacgcggcc cgggccgtcc gggccgcccg cccggtcgag gagggcccgc cggcgcttcg
    28981 gaagccggtc gagcggctcg cccgaatcgt cgctcgccag cgcgagcgcg gcggccagcg
    29041 ccgtctcggg gtcgtcctcg gccaggacgt cgatcaggtc cgggatcggg accaggccgg
    29101 ccggcacgca ggacaggacc gccgccttca ccgtcgccga ggggtgaacg agaagcggca
    29161 ggagcgtcgg ggcttccggg cctccacggt gtgcgtcgag gagctcgaga gcgggctcgg
    29221 ggtccgccac ctggaggacg cagagcagcg cttcctcgcg ccgggcgcga ggaagcgcgc
    29281 ggagcgcggc gtagccgtcg cggaatggtc cgtgggtcac gatcgccagc gcggcgcccg
    29341 gcaggtcgag gagggcgagg acgtgcgcgg cgtcggtccg gagcgcggcg tcctcctccg
    29401 ggtcctcgag gagggcgagg agcgtcgggc cggcttcggg ccagagcgga cggaggccgg
    29461 cgagcgtggt ggccgacgcc gactcgagcg ccgcgcgcag ggcggcgacg aggaagacgt
    29521 cgacctcgcc cgcggccgcg cgccgcagaa gcggctcgcc ggccacgggc gagcggagcc
    29581 gggcgagcgc gtgaagggcc ccgtggcgaa ggttcgcgtt cgtcaggagc gggaggatgc
    29641 gcggcgcgat ctcggggtcc ccgacgtcgc cgagtgcctc cagcgcgtgg acctgcagcc
    29701 agaggttccc ttcctccagg gccgcgagca gcggggcgat cgcctctctc cggccggtcc
    29761 ggccgagggc gaggagaagc gccgcacggg cgttcgggtc cccttcccgc cgcgcggcgg
    29821 cttcgatggc ggtcaccgtc tccggtccgg gagccgggcc ggggagcgcc gccgcggccg
    29881 agatccgcac gtcggcggac gggtgctgga gcgcgccgag gaggacggcc cgtcccgccg
    29941 cgccccccct caccaggagg gcgaggccgg ccgcccggtg accggcgtcg tcgtcgtcgg
    30001 cgagggccac cgcgcacgcc gacgccacgc gcggcgtgaa tcggcgaccg agcgcggcga
    30061 cggcccgctc ccgaacgcgg tagtcctcgt ctttcaggag cgccaggagg accgactcgg
    30121 cctcctcacc cgggatcctc ccgagctcac cggcgacggt ccatctctcg gtcgcgtcct
    30181 tcgacgcggc ccgggcggcg agctcgcgga tacggtcgtc gctcatcctt cgcctttcgc
    30241 cgcgagccgg tcgacgtcga ggagggagag gaaccccgac tcctccgcga cgatcccgcg
    30301 cagcgcgccg cgtcccggcg gcggtggcgc gacccggtcg gccggacagg tctccacgcc
    30361 gtcgatcgag tcgacgagga gcgcgtggtc cgttccgtag aggacgacga ccagcggcgc
    30421 gggcttcgcg ggagaccgcg gaagtcccat cgccgcggcg acgtcgatca ccccgagcgc
    30481 ctctccccgg accagggcga cgccgctcca ctccgggggc gcgccgggca gaggcgcgat
    30541 ccgcctcggg cgggcggcct cgcgcacgcg ctccagcggt accgcgaacc gctcgccgcc
    30601 cgcgcggaag gccaggagga ggccgtcgtc gctcacgccc gcctcccgga cgccgaagcg
    30661 gaatggacgc gcgcgacgag cgccccggcg tcgagggcga gggcgatctc gccaccggga
    30721 acctcggcgg cgcccgcgat ctccctcggc cgcggcacgg agtcggcgag cggcttcacg
    30781 aggaggtccc agaccccctc cacggcgtcg acgacgaggg ccgcggcccg ccggccgtct
    30841 tccgtgacga cgacgggctc cgcgcctccg gcgtcggggc gcctcggcag gtcgaagagc
    30901 gcgtcgaggc gcgcgagcgg cagggcctcg tcaccgtccc ggaaacaggg tctgccgtcg
    30961 atgacctcga tcgcgccctc cgcggcctgg agcgtccgcc tcacgttctc gccggggagg
    31021 gcgaaccaga cgtcgccgac cctcacgacg agcgactcca ggaggacgag cgtcatcggg
    31081 acgaccacct cgaacgtcgt ccccgagccg ggaaccgagc ggaccgagac ccgacctccg
    31141 agggcggtca gcttctcccg cacgacgtcg agcccaacgc cccgtcccga gatctccgag
    31201 acctcccggg ccgttgagaa gccggcccgg aagagcagcg cgaacggttc ggcgggatcg
    31261 gggtcggatg gaccgaggag accggtctgg cgggccttcg cgacgatccc cgccgcgtcg
    31321 acgccccgcc cgtcgtcgga gaacgtcagc acgacctcgc gcccccgcgg cgcgacggtg
    31381 agggccaccc ggccccggcg cggcttgccg gccgcggccc gctcctcggg tgactcgatt
    31441 ccgtggtcga gggcgtttcg aaggatgtgg agcagcgggt cgcccagctc gtcggcgagg
    31501 accttgtcga tctccgtctc gaccccctga acgacgaagt cggcctctcg cccgagcgcg
    31561 gcggcggtcc gcgcgaaggc gcgcgcgagc cgcgtcgtca gcgtctcgac cggcacgagt
    31621 cgaagcgcga gcgccgcggg cccgagcgtc gcgacgaggc gatcgagccg ggcgaaggcg
    31681 cgctgcgcct cgaaccgggc gccgcggtcg ggcgcgccgg cgagggcctg gccgagcgtc
    31741 cggcccagcg cggaccgcgc gagcgcgagc tccccggcga ggtcgacgag ggcggcgact
    31801 ttctcgagcg ggacccggac gacgccccgt gccacctggg gggcggcttc cggtcccggc
    31861 tcgggcggcg ccggctccgc gaccacggtg gcgggcaggc ggacgggctc cgtggcgggc
    31921 tccggggcgg ccgtccccgc ggagaccccc acgacgtcac gcgccgagca ccgggtcgcc
    31981 agcgccgcga cgtcgctgcc gggcggcagc gccacgagga ggcggaacgc catgcgggcc
    32041 gggtccgcgg ccgtgccggg aaacgtcccg atcagctcgc ccgcggccga cgcctcgttc
    32101 atcgcgttcc gcagcccggc gtcgaacgag tcgaagtcga ggtcgaggtc gacgaggacg
    32161 agagccttgc ctctcttccc cgcctcggac gcgcgatgcc tctcgtagtc cgaaagggag
    32221 gcgtcgagct cgggcggcag tcgaaggctc gcggcctcgc gcggctccgc gggacggacg
    32281 aggccctcga gccggtcctt cagcggcgtc tcgagcctcg gcgactcctc gcccgcggcg
    32341 acccgcgcca cgagcgtcga gagcgcgtcg aggccgtcgc gcacgccgcc gcgcaccgcg
    32401 ggcgacggcg ggacccgtcc catccggagg tcgtcgagca gcgcctcgag cgcgtgcgcc
    32461 gcgtcggcga tccccgagta cccgaccatc ccggccacgc ccttgaggga gtggacggtc
    32521 cggaagaggg cgttgacctt ttcggggcgg ggatcgtccc cctcggcgtc gagggcgtcg
    32581 atcccgcatc cggcctcctc gaggagcatc tccgcctcgg ccaggaactc cccggaggat
    32641 gcgtcgcccg gcctcttcgg gcgggtcacg gctcctccgg cgccttcgat cccaggacct
    32701 ccccgaggac ccggtcgaac gtctcgacgt cgaacggctt gccgaggtat ccgtcggccc
    32761 ccgccttccg cgcccgcgcg gcgtcctcgg cgtcgcgctc cgtcgagacg gcgatcaccg
    32821 gcagggcgct ctgctccggg cgcgagcgga tcatccggat cagctcgagg cccgagaggg
    32881 tcggcatgtt cacgtcggtg aggacgaggt cgatcggcga gcgcggcagc tcccggagcg
    32941 cctcgaaccc gttctcggcc tcgacgacgt cgaccttgtg gcccgtgcgt cgcgcccacc
    33001 cttcgagggc ctgccggacg aggaggcgag cgggtccgga atcgtcgacg acgaggacgt
    33061 gtcgacggat cggtcccgac gcggtcatcg ctggggctct cccggaggga ggtccaccgc
    33121 ctcgacgacc cggagccggg ggcgggcgaa cgagccgtcg cgcaccttcc acgcgagggt
    33181 gggcccgcgc gcgaggatcg tcagcccgaa cccatcgggc ggcgccgccg gcagggccag
    33241 gtgtcgggcc gtcagcatgt tcgcgagccc cccggggtcc gacgggaccc acccgaggcc
    33301 cggcacgaag acctcgatcc agcggtgccg caccccgctg atcgtgtcgc tgtagtgctg
    33361 ctgggattcg tcggtcagct cgctcggcgt gcgggcggtg aggatcccgg tgacctgccg
    33421 gaccgggatg ccgccgcggc ggaggaggtc ggccgcgagg agcgaccggc cgacgcacga
    33481 gccccgccga ctccggtggc aggaggcggc cgtctccgcg aggagactcg gcgactcgta
    33541 gcggatccgc ttcgagaccc aggcgacgac ccgctcgacg gcctcgagct gcgtcgtcgc
    33601 cccgcgcagg aggatccggg acaggtcctc cagctcgtcg tctgcgtcga gcggctccgc
    33661 caggagcgcc ctcgcctccg ccgggagggt ccgagggtcg accgggtagg gtgccgtgtc
    33721 gcgaaggggg gctccgtcca cctcgaccgt cgcctcgagc gttcgcccgt cgggcgagga
    33781 gaggacgacg cggtatccgg gcgcctcgac ggggcggacc gcccgccacg gctcgatccc
    33841 ttcgacccgg tagcgcgccg tctcgacggc cgcccgctcg ggccccgaga ggaggaggaa
    33901 cgcgagcgcg aggggcgaga ggttcatcgg cttccggccg gcgccggccg cgagcgccgg
    33961 agcgacgcct cgcgcgcccc gaagaggttg cgtcggatgc cgtcgaggac gccgttgatg
    34021 aacggcgcgg acgacggcgt ggagaacttc ttggcgatct cgaccgcctc gtcgatgacg
    34081 accgggtcgg gggtctcgtc ctcgtgaagc agctcgtaga gggcgacccg gaggaggttc
    34141 cggtcgacga ccggcatccg cccgaggcgc cagtggtccg actgcttccc gagcagctcg
    34201 tccagctccg agagccggcc gagcgtcccg aggacgagcc gcgaggcgta ctcctggacc
    34261 tcgggcgcag cgttccggta ctcgtccgtc cgggcgaaca tctcctcggg agaggtcccg
    34321 gcgatgtccc gctggtagag gagctgaagc gccagctccc gggcccgccg cctgcggccg
    34381 ctcacggcga cctcctaccg gaccggcgcg cccgcccgca gccgcgcgcg gaggtccgcc
    34441 agctcgacgg cggccgtcgc cgcctcgaac cccttgttgc cccccttcgc gccggaacgc
    34501 tcgagcgcct gctcgagcga gtcgcacgtc aggacgccga acgtgatggg aaggtcgagc
    34561 tcgagcccga cctgaaacac tcccttcgcg acctcggtcg cgacgtagtc gaagtgaggc
    34621 gtgccgcccc ggatcacggc gccgagagcg acgagggcgt cgaagccgcc ggacctcgcg
    34681 agatcgcgca gcgcgagggg aacttcccac gagccgggga ccttcaccac ggtgacggcg
    34741 ccctcggccg cgccgagccg ccggaaggca tcgagggccc cgtcgaggag gcgggagacg
    34801 acgatgtcgt tggtccgaga ggcgacgatg ccgaaccgcc gtccgtcggc gcgaagctga
    34861 ccctcgagca cgttcatgac cgcctcagtc tagcagagag aagcgtgccg ccggacggga
    34921 ggcctccgtc ccggcgaggc ctcggagccg ctcggcgagc ggctcgacga ggggcttccc
    34981 gaagctctcg gcgtaccgct cgaggagctc gtcgaggctc cagcgccgcg gctggggacc
    35041 gagcgtctcg aggcagagga cggccgccac ggcgccgacc ttcccggcct cctcccaggg
    35101 caggccgcgc aggcgagagg caacgagccc ggaccggaac gcgtccccga cgccggtcgg
    35161 gtcgagggcc tccgtccgca gcttggccgc ggggacccgg accgtccggg cgccccccgg
    35221 ctcctccggg gagcctccgc ggagggcgat cgtcgccccc tcctcgcccc gcgtgatgac
    35281 gaggacgggg accttcgcgg cgagctccgc ctccgagagg ccggtcttct tctcgatcac
    35341 gccgagctcg tactcgttgc cgatgagcat cgcggccccc tcgaggcccg cgagcaggtc
    35401 gtcgccgtcg agccgggcca cctgctggct cgggtcgtag acgaacggga tgccgagctc
    35461 ccgacactcc gccgcgtgct gccgcatcgc cgtcgggtcg ctcggacaga tcagggcgag
    35521 agcgaccgag ccgggcgcga aggagcggaa cgtcagctcc gacgtcttcg ccatggctcc
    35581 ggcgtagaac gtcgcgatct ggttctgctc ctcgtccgtc gagacgaaga acgacgccgt
    35641 gaagacgtcg tcgcagagct tcatgccgga gacgtcgcac ccctgcgccc cgagccacgc
    35701 ccggtagtcg gcggcgtcct gccccgccgt cgcgaagatc gccggcgtca tcccgaagag
    35761 cgccagaccg taggcgatgt tcggcgcgac gccgccgcgg acgcgccgca tctcgtccac
    35821 gaggaacgag accgacaggc gatgcatccg gtccgggacg acgacgtcct ggaacttgcc
    35881 gggaaagcgc atcaggtagt cgaacgccac cgagccagtg acgacgacct tcgggctctc
    35941 gctcatctcc agtacctttc catcagcagc gcgaggcgcg cgcgcgcggc cggcgggatc
    36001 gccgacggcg cggtcaggac cgcgtggcgc atggcgccgt cgcagtcggg cggacgatcc
    36061 gggtcgaggg aggcgacggc ccgcgcgagg acgcggtcgg ccgtctcggc gttcctccgg
    36121 aggacggcga ggacggcctc gacggagacc ggcgcctcct cctcgtgcca gcagtcgtag
    36181 tcggtcacga ggcacatcgt cgcgtagtcg atctccgcct cccggcagag cttcgcctcc
    36241 gtcgcgttcg tcatcccgat gacgtcgacg ccccaggagc gatagagacg cgactcagcg
    36301 agcgtcgaga actgcgggcc ttccatgcag aggtaggtcc cgccgcggtg ggcgacggct
    36361 cccgtctcct tcgcggccgc ggcgaggagt ccgaggaggt gctccgagac cgggtgcgag
    36421 aacgagacgt gggcgacgca gccttcgccg aagaaggtcg acgtccgccc cttcgtcagg
    36481 tcgaggagct ggtccggcag gacgatgtcc gtcggccggt actcgagctt catcgaaccg
    36541 caggcggacg aggagatcag gtacgagcag ccgagcttcc ggaagcccca gacgttcgcc
    36601 cggtagttca cctccgacgg aaggaaacgg tggccccgcc cgtgccgggc gaggaaggcg
    36661 accggcaccc cggagagctc cccgaggacg aatgcgtccg agggatcgcc gaacggcgtc
    36721 tcgagcgccc gctcctcggc gaccacgagg cccggcaggg cgtagaggcc ggagccgccg
    36781 acgaggccga tccgcttcct caaccgatcc tccgggcgac ttcctcctgg acgtcgaggg
    36841 cgagcttcag agccgcccga ccggcctccc ccgtcaccgc gggctccgtc cgggtccggc
    36901 acgcctcgag gaacgccccg tcctccgcgg tcagcgggtc ggtcggcacg acctcgaccg
    36961 gccactccat gatgcgcggc gggccgctcg tccggtcgag gcgataggcc gtcacgtccc
    37021 gcttctgggt gtcgatcgag aagtaccagt cgggctggaa gaagcggaac ttccgggtct
    37081 tctccgtcga gacgcgcgag gccgtcaggt tcgcgacgca gcccccctcg aactcgatcc
    37141 ggatcgaggc gatgtccacc ttcttcgaga ggatcggcac tccgaccgcg tggatcgccg
    37201 tcaccggctt cttcacgatc tcgagcgcga tgtcgaggtc gtggacgagg aggtcgagga
    37261 cgacgtcgac gtcgagcgcg cggggaacga agacgccgag gcggtgcgcc tcgatgaact
    37321 tcggctccgt gacgagcggc aggaccgcga cgacggccgg gttgaaccgc tcgatgtggc
    37381 cgacctggag gacgcgcccc ttcgcgcgcg cgatggaaat gaggcgatcc gcctcctcga
    37441 gcgtcaccgt catcggcttc tcgaccatca cgtcggcgcc gcccgagagg agctcttcgg
    37501 cgacctcggc gtgagtcgac gtcggcgtcg cgacgatgac cgcggaagcg cccctctcga
    37561 ggagctcctt cacggagcgg cagcggacga gccccgaggc gccgtgcttc gactcgatct
    37621 cggcggccat gtcgtcgttc ggctcgacga atccgacggg ctcggaaagg gggagctcgg
    37681 tgaggatgcg ggcgtggtgg cgtcccaggt agccgcagcc ggcgatgccg gtcctgatct
    37741 tctcggacat caaacccggg agtttatcgg agcggtgtcg atcccgacga tatcgaggag
    37801 ctcgcccagc cgttcgatcc gctcggcgcc gccgtgaccg aggccgacga accgaaccgg
    37861 cgccgcgatg gcggccgccg cgtcgaaccg cgagtcgccg acgaacaggc actcgtcgag
    37921 cggcgtcccg actcgttccg cggcgaggcg cacgacggcg gggtcgggct tccccgctcc
    37981 cgcctcgtcg gccgacgcgg tcgcatcgag gagcggaagc aggccgtgca gcgcgaggac
    38041 gcgccgggcg agcggcgccg tcgtgttcgt tgcgaccgag gtccggatcc cccgacgccg
    38101 gagctcggca agaacggccg gggccgccgc gttcgggcga atttcggcga ggtgctcctc
    38161 gaagagccgc gcgtaggccg agtcgatctc cgcaaccgag cgctcgggca tgtaggcgtc
    38221 ccggtcggcc gccgcgccgt tcccgtagat cgcgtcgaac tcggcccgcg agacgggccg
    38281 gtggccgaag caggtgagcg tctcgttgaa gaccgagaac caggcgtcct ccgagaagac
    38341 gaggacgccg tccatgtcga agaggaccgc gcggacggga acgggcaagg gcacctccgg
    38401 gcggcggatc atcgcagagg gcgggcagcc tgcgcgcttc aacgcacacc cgcgctctcg
    38461 aaggcgtcgg cgagacagcg cgtcgcgctc cggcatcgct tcgactcgca gccgccgcag
    38521 ggcgcgccgg ccgatcgggc ccgcggaccg cggagcccgg cgcgggcagg gttcgtcggt
    38581 ccgaggatcg aaagcgactc ggcgccgagg agggctgcca tgtgccccgg acccgtgtcc
    38641 gccgagatga cccgctccgc cgcgagggcg agccccaggc tccctaggag cggaatcgtt
    38701 cccgcgcagt tcaggatccc ctcccggcga ggccgatagc cggcgagccg ttcgtctccg
    38761 aggaggacga gcggcgggcc gggccagcgc tcgagccact cgggcgggat cgaccgcgac
    38821 agcccggacg acatggggaa cgcggccgtg aaccgccgcg gaaggccgag ccgctcggct
    38881 tcccgggtcc ccgcggcgcg cagccgcgcg gccaacgggg cgagaccgcg ctgcgcctcc
    38941 tcccagcctt cgcgaaccga cgccccgagc gcctcgaccg cgcgcgccgt gttctcgatc
    39001 gcgtgaggcg tccgccggtg gtcgtacatc ggcacccggc ggtgcagcca gggccagcgg
    39061 ccgcgaccga cgcggcgatc cgccccgctc cagacggtcc agagcgagga gcggaggagc
    39121 ccctggaggt cgacgacgag gtcgtagcgc cgcgcgacga ggcgcgcggc gacgcgctct
    39181 cgctccgcga agagctcggc ccagcctgcc gcgaaggctc cgcggacgtc cgcgacgacc
    39241 gtctccgcga cccacggcag ctcggcgacg aacggcgctg ccggcggctc gacgagccag
    39301 tcgatctccg cgcgcgggaa gcgccgcgcg agggccgcca ccgaggggac ggcgtgcgcg
    39361 acgtccccga gagaggagag cttgacgacg aggagcctca agggccgcat tctcgcctcg
    39421 cgtgcggcgg gcgtcgtcgt cctctcggag taacctcgga cgtgcctcgc gtcgccgtcg
    39481 tcatcgccac gcatcgccgg ccggcgctcc tcgccgaggc cctgtcctcc gtcgcggcgc
    39541 agacgttccg cgacgtcgag acggtcgtcg cggaggacgg cggcaccgag gagactgctc
    39601 gcgtcgtcgc cgcctcaggc gcgcccggcg cccggcacgt tcccctcgcc ttctgcggaa
    39661 ggcccggccc cgtgcgaaac gaggcgctcc gccgcgtctc gtcccccctc gtcgcgttcc
    39721 tcgacgacga cgacctctgg cttcaccgca ggctcgaggc gcacgtcgcc gctctcgagg
    39781 ccgctccggc cgccgggctg gtcttcggcc cgatacggcg cttcggcgac gaggagggca
    39841 cgtggccccg gcacgttccc ccgcgggtcg acctcgcccg gctcctgcga gggaactgtg
    39901 tccccctctc ctccgtcctc gcgcgtcagg aagcgctcga acgggccggc ctcttccccg
    39961 acagggcgga gctggcccag gactacgagc tctggctccg cgtggcgcga tcgcacgtcc
    40021 tcgtcgggca ccccgagccc ctcgtccgct accgcgtcca caccggcggg atcagccgga
    40081 agaaggccct cgaagtcgag gctgtggccg cgatcctcac gcgcctggaa tcggagtggg
    40141 acctcccgcc acggctcctc gcccccgcac gccgcggcct tctccgatcc cgcgcccggc
    40201 tcgcccccgg cctgcgcgag gcgatccggc tccgcgccct ctccctcgcg ccgggaccgt
    40261 tccttcgcgg ctgacgccgc ttcgaggggg gcgaaccccg tcgtccacac ggctcggacg
    40321 ccgccgcgtc agccgacgcc gagccagtcg accacgtcgt cgaggtgacg ctgcgcgtcg
    40381 agcgacggcc ggaagccgag ggcggcctcg cgcagctccg cgcgccgggt cggctccgcg
    40441 atctcctcca tcgcccgcgc gagcccttcc acgtcccgcg gatcgtccac gacgagcgcc
    40501 gcccccgccg gtccaagcct ctcgaccgcg ccgtttctcc gggtcgtgat caccggcacc
    40561 ccgcaggcag ccgcctcgag acaggccagc gagcaggggt cgaggaacgt cggatggagg
    40621 aggccggtcg acgcgcggag ctttcgaacg acgtcccccc gagacagcgg accttcgaac
    40681 gtaaccgcct ccgcgacccc gaggcgagcg gcgatacgcc gccacgcgtc gcgaggcacc
    40741 cgccccacga cggacagccg ggcggcgagg ccgcgccgcc tcgcgacggc cacggcctct
    40801 aacgcggtac gcaacccctt caggcgaggg ttgtgggcga ggaagaggaa ctccgcggct
    40861 ctcggccgga agccgtccgg atccggcgag aagagcgcga ggtcgacgcc gggcggcgcc
    40921 acccggatcg ccccgtcggg aacgccgagg acgcgcaggc ggctcctcag cgcctccgtc
    40981 cagaccatga tcttcgggca ggcgcccgcg agcgcctccc tctcgcggcg aaggaggagc
    41041 cggcgtcgcc cgttgagggc cgtcccgacg tcgaagagcg cgcgccgcac cccttccatc
    41101 gactcgcgct ccgcctccag ggcgtcggcg aagaggccgc cgtgcagctg gacgtgcgtc
    41161 gcgaacggga ccagcctcgg cgcgaggacg ggcccctctc cggcctcgag gcggcgagcg
    41221 gcggccgcca ggaaacgccg ttcgccgaag acgcctcctc ccggggggac gagcgcctcg
    41281 acggaggtcc cgtcgccctt cccggcgccc tcgcgacaga gcacccgaat gccgactcct
    41341 cgccgcacga gctcaccgcc cagcgcgacg aggtatccct cctgccctcc gcgcgaggcg
    41401 tcccagcggt cgacgagaag ggtcgccctc acatgcgccc gcgctcggca cgggtccgga
    41461 gggccgggaa acggcatcga ttcgtcgtca tctccccgag catgccccac cgtccccgcc
    41521 tctcctccgg cgcatccgca ggacaccgcc tcccgcgcga ctgtctcacc tccttccgga
    41581 cctcggcaat tccttctcta gctcgcctcg tccgggcagg tcgcaaacta gaatcccggc
    41641 ctcatgggac ggcaccacac ggctctcgtc gatccgggcg cgcgactcgg ggccggggtg
    41701 gaggtcggcc ctttcgcggt tgtcgaggcc gagacgggca tcggcgacgg atgcgagctg
    41761 ggcgcccaca gcgtcgtcaa gcgatacacg tggatgggcg ctcgcaaccg cgtccacgag
    41821 ggagcgatcc tcggcggcgc gccccaggac ctcggtttcg acggaggtcc gagccgcctg
    41881 gagatcgggg acgacaaccg gatccgtgag ggagtgacga tccaccgcgc gacggctccc
    41941 gggggcgtga cgcgcgtcgg ctcgggctgc ttcctcatgg cgtattccca cgtcgcccac
    42001 gaagacgtga tcggcgatca cgtgatcctg gcgaacaacg tcgcgctggc tggacatgta
    42061 cggatcgacg agcgggcctt cctctcgggc ggggtggtcg tgcaccagtt ctgccgggta
    42121 gggcgcctcg ccatggtcgg cgggaacacg aaggtcatca aggactgcct gcccttcgtg
    42181 atcaccgacg gcgtcccggc ccgcgcgcgc ggcctgaacg tcgtcggcct ccggcgggcc
    42241 gggttcacgg cctcgcagct ccggacgctg aaggaggcct accggctcct cttgcgctcc
    42301 ggccttccgc tggcggaggc cctcgagaag ctggaggcgc tcggcgaccc tctggcggac
    42361 gagctgggcg ccttcgcgcg cgactcgaag cggggcttcc accgctcgtc gcgcgagatc
    42421 gacctcgcct agcaggctgg ccgccggcct cccccgcggg accggtgcgg ccttgacgga
    42481 ctgcgtggcg taccggcatg gtccggccac ggtggacagc ttcccgccct ccgggagccc
    42541 gaggggcttc gacgccgcgc gtgcggccca cccgacacgg gtgctccgaa tcgaggaggt
    42601 cgtcgacctc ctcgagctga cgcacgccgc ggaggtgatc cgcgggtatc gcgagaggat
    42661 gcgcgcggga gatctcttcc cgccggtctc cgtcgtgcgg ctcttcggga agtggctcgt
    42721 cgccgacggg cacaagcgct acgtggcgtg gacgggcctg ggagagaagg agatcgtcgt
    42781 cgaggtctgg ccgctgggtc gctggctggc cgaccaggcc ggccaggcga ggcgcaacgc
    42841 gcggaagaac gggaggatcg ccgcccgtct cttcgtcgac ccgcgcgagt cgctccgcct
    42901 ggcccgaacc acggcggggc actggaggcg cgtggcgcgc tccctgctgc ggcgggcggg
    42961 ccgacgcggc aagcgacccg cgagtcttcc ggcgtgagcg acgggatcct ggcaccaggt
    43021 gccggacggc gcctgctcct tcggatcgcg agggacgcgg cggtcgagcg gtcgcgattc
    43081 gcgggggcgg cggtcgcgct cgcgacgatg gccggcgccc agctcctcct gacctggctc
    43141 gtcaaggagt ggctggaggg gccgctcacg ggaggctcac tcctccccgt tcgcgccctt
    43201 ctcctcgagg ccgcgggcgc gaccctcctc ctcgcggcgg cggtcgtcct ctcgcgctac
    43261 ctcgcggcga gcgtcaacca gcgcctcctc gagcgcctgc gcgaccgcgc ggcgcggcgg
    43321 gtcctcgcga gccgggccct cgaggtgaga ggccgcccgg ccggcgagct cctctcgcgg
    43381 gtcttctcgg acgccagcag cctctcgggg ttcgtcgaga cactgctgaa gaggctcgtc
    43441 ggcgacggcc tcgacgccct cggctcgatc gccgtggctt tcttcgtcga gtggcgcctc
    43501 gctctcgggg ccgtcgtcct ggcccccctc ctcggactcc ttctcgaccg cctgggacgc
    43561 gtcgtcaggc gcaaggggag cgtcgcccag aggaagctgg gcgagcttca cgccgtcttc
    43621 tccgagcagc tctcgggcct gacgaccatc cagggatacg acgccgccgg gcgcgaggcg
    43681 gatcgcttcg cggcgacgaa cgccacctat cgccggagcg tcctttccgc cgagctctgg
    43741 tccgcgacgg tcctcgcctc gatcttcctc gtgaccggac tcgggttcct cggtgcgatc
    43801 ggctggggaa gccggctcgc cctcgcgaac gagctgacac cggccggtct cctggcgttc
    43861 tgcctcttcg ccgcgcggac ggtcgagccg ctccggcgtc tcgccgacgt ccacgcgacg
    43921 ctccagcgga ccctcgccgc ggccgcgcgg gtctacgagg tgatcgactt cggcttcctc
    43981 gaacggcagg gcgggacgcc cctcccgcgg cccgtgcgcg ggaggctcgc gctcgaaggc
    44041 gtcggtttcc gccacgagcc gtcgcgcccg ctcctggagg gagtcgacct ccgggtcgag
    44101 ccgggcgaga cggtcgccgt cgtcgcggcc agcggcggag ggaagagcac tctcgcggcg
    44161 atgctcgcgg gcttcctctc tccggacgtg gggaggctcg cgctcgacgg cgtggagctt
    44221 cgcgaagcgg accttcgcga cctgaggcgc gccgtgcggc tcgtcgagca ggagccgttc
    44281 ctcttccggg ggacgctggc ggacaacgtc ctcttcgggc gcccggaggc gtcgcggagc
    44341 gaggtcgtcg gggcgctggg cctctgcggc ctcgagagcc tcgtcgccgc gatccccggc
    44401 gggatcgacg ggtcgttgct cgaggccgga cgaaacctct cgggcggcca gaagcagagg
    44461 atcgccctcg cgcgcgccgt cctcaccgac ccgccggtcc tggtccttga cgaggcgacg
    44521 agcgcgctcg acggcgagac ggagggccgg ctcctcgacg atctcgggcc gtggctcgcg
    44581 cgacggacgg tgatcgtgat ggcgcaccgt ctctccaccg tggcccgata cccgagagtg
    44641 atcgtcctcg tcgacggccg cgtcgcggga gacggtccgg catcgcgcct gctggagtcg
    44701 tgtctcccct tccgggcgct cttcgcggat caggtcgagc ccgtcgcgaa ccgcgcgacg
    44761 tgagcgtgag cggggtcgtc cccgcgaatc cgggtcggag ccgtcagagg ggctcgggcc
    44821 cggtatgccg cgccgaggcc agaagaccga ggatcctcgt cgccgttcgc gcggcggcgc
    44881 cccggaggga accgagagcg cggcgcccgg cctcgccccg gcgaaggcga tcaccatcgt
    44941 cggagaggag ccgcgccaac gccgctgcga gctcctcgac ggccccgtcc ttgcccacct
    45001 cgacgagcgc tccggcgtgt cggagcgcgt cggccacgtc gcgtacccct tcgagagaag
    45061 gcccggccag gagcggcacg cccgcctccg cggccgcgac ggggctgtgc ccgccgaccg
    45121 gagcgaaggt cccgccgagg aaaccgatcg cggcgtgcgg ccagagcgag gagagcaggc
    45181 cgaaggtgtc gacgacgagg atcgaagcgg ggcctggagg gcccgagagg cggcacgtcc
    45241 cgaagccggc ctggcgggcg aggcgctcga cctcgctcga cctcgcgacg tgccgcggcg
    45301 cgatcgccag ccggacctcg gcgctttcgc cgcgcagcct cttcacggcc gccaggagga
    45361 gctcctcctc gggagcgtgg gtgctcgccg cgacgaggag aggagggccg atcgcgagcc
    45421 aggcttccca cccgggcggc agcgccgcgg ccggcgtcgg ggcgtcgagc ttcagatcgc
    45481 cggtcacctc gacgcgggag gggctcacgc cgatcgcgag gaacctctcg cggtcctcct
    45541 ccgtccgcgc gccgatccac gcgaccgcgt cgacgacggg gcggaagaac gggcccgccc
    45601 gcgcgtaccg ccacgcctcg cccggccgga gccgcgcgct cgcgacggcg accgggatgc
    45661 cgagcgaccg tgccccgagg aagagcccgg gccagagctc ggcctcgacg gtcaccacgg
    45721 ccgcgggagc gagtcgcgcg agccaccggc gcaccgcgcc cggccggtcc cacggcagga
    45781 acgcgaccgc ctcgaccgcg cggcacgtct cgcgaatccg ctcggccacg atccgcccct
    45841 ctctcgtccc ggtcgtcagc agcagcttca ccccgcacgt ccgctcgatc tccgacgcga
    45901 gggccgacgc ggcggtcatc tccccggccg aaacggcgtg gaggaggagc ctccccggac
    45961 cgcacggtgg cggcgccgtg ccgaggcgct ccgcccgctc ggaggctccg gccccgcgaa
    46021 ggagcgcgat ccgcgccgcg gcgaccgccg ccccggcagc cactccgtac gcaccgagga
    46081 cgaagccgtg aaaccgggag agaccgggct ctctcacgga cggtccctcg ggagacggca
    46141 gacctccgag cggcggcgtc tccgcggcgt cgtcccgtcg cgcgacgacg tcggtcaccg
    46201 cggggccatc gtccacggtc caccgcgcgc cagagcgacc agctcgacgg gctccccttt
    46261 cgagacgttc atccctccct gccgcgccac cgggacgagg ggcagcaggc cctcgcgcgc
    46321 caccaggcgc tcgagcccgg ctcgcgtcgt gacgaggagt cccgggcctt cgccgcgcgc
    46381 ccagcgggcg gcgtccgcct cgctcgcgag gcgctccacg ggtcccggac cgaaatagac
    46441 gaagacgctc ggctcctcga acccgagcgt cgcggcgggg gcgtcgccca cggcacgccg
    46501 cgcctcggcc gcgaggcggg gaaccggctt ccacctctcg agcgcgggaa gaccgaatcc
    46561 cgccgtcgcg acgagcgcga cagcggtccc cgcgagaagc gtcgcggcgg cggctcgcgt
    46621 gtgtcccgac cgcagaagca ccagagccag ggcgacctcc gccgcgatcg cgacgatggc
    46681 cacggccgcc ggagcccgca gtgccgccag cggggggacg gtcacgatcg cggtgagggc
    46741 cgcgagcgcc gcgacgacga ccggagcgag gaggagcccg ccggcgacga gaccgcgtcg
    46801 ttcgcccgcc cccgcggcct cggccgcgag aagggcgagg gccgggaacg ccggaaggag
    46861 gtagtgcggg agcttggtgg cgacgacgct gaagaggagc agcggggcga ggacgcttcc
    46921 gaggaggatc gcgcgggccc cgggcgcgag cccgccgccc gaccggacgc gggcccaggc
    46981 ggagggcagg aagagcgtga acggggagaa gccgacgaga aggacggcgc cgtaatagag
    47041 cggccccacg aggagtccgc tcccgtggcc ttccatcggc gcgagcgtcc ggccaatcac
    47101 gtgcgtcccg atcgcctccg agaggaggcg gccgttcgtc gcggcgtcgg cgaggagtcc
    47161 ccagccgaga accggcagga ggccgaggat cgaggatgcc gccaggagaa cggcgaggcg
    47221 ccgtctttcg cgaaaggccc ccggcaccag caggagcgcc ccgccgaggc cgaggagcgg
    47281 aagcgcgaac gcgacgggcc ccttggcgag ctgcgcgacc gccgaggcca gcgccccaag
    47341 gacgagatgc cggggccgga cgccgccggt cgcggcgtcg gcgaagaccg cgaggactcc
    47401 cgtcagcccg gcgaggagga gggcgtcggc cgtcgcggcg gccccctcca ggagggcgag
    47461 aggcgacagg gcgagcagcg ccgctccgag gagcggggac gtcccgcccg caaggcgccg
    47521 gccggcggcg aaggtcagga ggacggaggc accgtgcgcc agcgcggacc agaggcggaa
    47581 ggccacctcc gtggggccga ggacgctcgt cgagagggcc ataagccacg gaaggagggg
    47641 cggcttgtgc ggccgcagct cgcctgcgaa cgtcgggacg aggaagtcgc cggacgagag
    47701 catctcgacc gccgagcgcg cgaaacggga ctcgtcccgg tcccagagcg tcgttcgcgg
    47761 cgcgagcagg agaagcgccg ccacgaccgc ggccgcgagg gcgaacgccg cgcggggggc
    47821 gaaacgctcc tgtgaaaggt gactgcggct catgggacgg cggccgagtc tagcgaaggc
    47881 gccggagcac ttgccccgat ctgcccgatc cgacgcccct ggcgttttcc ggacttgcta
    47941 caattgccgc actcaagacc gttcccggaa tctggagggc ccgaatgagc cgaaccactt
    48001 cgtgcttcgc cgccactgcc gcgctcgcgt tccttttctc cgcgagcgca ttgatggctc
    48061 agcccacacc gacgcccaca ccgggaccgt ccgccggacc cggtccgtgg agtgccagca
    48121 gccagatctg gttcgacatg aacggggatg gagtgccgca ggcgggcgag cgaatgggct
    48181 acccccagcg gcaaacgacg gattgcaccc aggtcgtcgg gctcccatcc gggatccccg
    48241 gaaccgggac ggagaccccg tgcatgaccg tctaccagac cgagggtatc tccgcctacc
    48301 gcacgaggcc ggacggcacc gttcagtcgc tcttctcgaa cgaagcgggg accctcttca
    48361 cgttcaccca gactccgtac gtcccgcctg cggccgccgg cggagcgcgc gccctcgcct
    48421 cctcggccag cgggacgggc cagctcctcg accagaacgc ggacgggatc tacgagacga
    48481 tgcaggtcga agggaccgga gtcccccaga cccggatgag cctggtcccg cgggacacga
    48541 cgggagacgg acgccccgac ttcatcaccg tgccgtggac gacgagcggc gccggacttc
    48601 tcggcgtcgc caccgcgacg acgccgcaga tctacttccc cctgacggac acggacgcgg
    48661 acgggtggcc cgacacgatc acggtccagg aggtgggcgg gatctcgaca tcgaccggcc
    48721 cgcccctctc aggggccgcg ctggccgccg ccgccgtcgt cccgtcggct tcgaccttcg
    48781 gtctcttctt cttcgccgcg gccgtggtcg ctctcggcgt gaagctcctc cgcgggaccg
    48841 ccaccgccct ctgaggcccc cccgatcgtg cggttcgtcc tcgtcgacca gttcctctcg
    48901 gtcgagccag ggcgaggtgc gacggcgctg aagacgttcc gccccgatga ccccgttttc
    48961 gcggatcact tccccggatt cccgatcgtc ccgggggtcc tcctgacgga gtcgatgggg
    49021 caggcggccg ggtgggctct cgccacgaag gtcgggccgg accggttccc cctcctgatg
    49081 atggtcgagc gggccaagtt tcggcgcctc gtccgtccgg gcgacgagat ccggctcgag
    49141 gccgacctgg aagagacccg cgaggacgtc tggacggcac ggacccgcgc ctcggtcggc
    49201 gacgagaggg tcgccgacgc gaggctcgtc tttcacctcg tttcgaggcc ctcgggttcc
    49261 gccgaattcg acgcgtggag ggcgggaatc ctccgcgaga cgggtctcgg ccgcctcgtc
    49321 gagaccgcct tatcctcctc cgcggaatga agcgccggct gggcctgttc ctcctccttt
    49381 cggggacgtt cgtggccgcg cgcgtcctcg tggcgcgggc ggtcgacgac cgaaacccgg
    49441 agacgaggct cctcgcgctc cacgcggttg ccgtgccgct cgcccaactc gctctcttcg
    49501 aggccgccga acgtctccgc gcgcgaagga agggcaccgc gtgaggatcc tcctgagcgc
    49561 cggagaggtg tcgggagacg tcgctggcgc gcgactggtg cgcgcgctga gagcgcgtcg
    49621 ttcggacctc gctttcttcg gtctcggcgg cccgcggatg gcggaggccg gcgtcgagct
    49681 cctccggagc acgaacgccc tcgggaccgt cgggatcagc gagtcgttcc gggtcgttcc
    49741 gggcctggcg cgcgcgttcg cgtccctgcg ccggcgcgtc gcgggttcgc cgcccgacgc
    49801 cgccgttctc atcgccaacg acgtcttcca cgtggtactc ggtcgatggc ttcgggcccg
    49861 cggggtcccg accctctcgt acttccctcc gcaagtctgg atctggcgct cgctcgcccg
    49921 cgtcttcgcc ccgagcttcg acgagatcct gacgtcgttc cccgacgagg aacgcgtctg
    49981 gcgagccgct ggggcgtcta cgacattcgt cgggcactac ctcggcgacg tcctgcggcc
    50041 cgccacggaa gcggaacgac ggcacgcccg cgaggccctc ggactccccg ccggaccgct
    50101 cgtggcgatc cttccgggca gccgggagct cgaggtgcgc cggctcaccc ccgtcctcct
    50161 cgacgccgcg cggctccttc acgagcggga cggaatggtc cgcttcgtcc tccctgtcgc
    50221 cgatcccgcc ttcgcgcccc ggatcgaggc cgaagtggcc cgccgagggc tcgggggagt
    50281 cctcggaacg acctccagcg gccacgacgc gctccgcgcg gccgacctcg cgatcctcgc
    50341 ctcggggacc gcctccctcg aagccgccct catcggaacg ccgatggtca tcgcctaccg
    50401 tctcgccgcc ctgacgatgg gcgtcgtccg cagcgcgatc gctctcggcc tcatcgactc
    50461 cgacacggtc ggtctcccga atctcgtcct cgggcggcgc gcgattcccg agcacatcca
    50521 ggaccgggcc agcggccttg cgctgtcggc cgcggcggtg cggctcctcg aggacgattc
    50581 tctcctcgag gagcagcggc aggcgctcgg ggaggtgtcg cgcctcctct tcgcgggcgg
    50641 cagcgacgcg cgggcggctg aggccgtcct ggcggccgcg gagcggcgcc ccgtgcgggg
    50701 cgcggccttc ggcggcgcca tcgtccggga ggaagggagc ggcacgtgag gaagacgacg
    50761 ctcggcgaaa tcgccgttct cctgggaggc gagatcgacg gcgaccggga gactccgatc
    50821 gaccggctcg agcctccgga ggaagcgggc gagggcgcgc tcgccgtcct cgcggaccgg
    50881 aaggcgctcg ccgggatcgc ctcgtgccgg gcatcggccc tcgtcgtccc gccagggacc
    50941 gaggcgccgg ggcgttccat cgttcgggtc ccttaccccc gccgggcgtt cgtcgctctc
    51001 ctcgcccact tcgcgcccga cgacgcgcgc gggccgggag tcgatccccg cgcgtacgtc
    51061 gacccgggcg cggagatggg agaaggggtc tgggtcggcc ccggggccgt cgtcgaggcc
    51121 ggggcccgcc tcggcgcgcg cgtccgcgtt cacccgctcg ccgtcgtcgg agaaggcgcg
    51181 agggtgggcg acgactcggt tctctttccc ggcgtcatcc tctaccccgg cgtgacggtc
    51241 ggacgccgcg ttcggatcca cgcgggaagc gtcatcggga gcgacggctt tggctatgag
    51301 cgcgacgctg gcggagtgca gcacaaggtc ccgcagatcg ggaccgtcga gatcgccgac
    51361 gacgtggaga tcgggtcgaa ctcctgcgtc gaccgcgcga ccgtgggcgc tacgagaatc
    51421 ggggcgggga cgaagatcga caacctcgtc caggtcggcc acaacaccca ggtcggcgag
    51481 cactgctgcg tcatcgggca ggcgggcctc tccggaagcg tgaaggtcgg gcgcctcagc
    51541 atcctcgcgg gccagtccgg catcgcggac cacgtcaccc tcgccgaggg gacggtcgtc
    51601 ggcgcgcagg cgggagtcca ccgcgacatc ggccccggaa tctggcacgg cgcccccgcg
    51661 cttcccgcgg cggaggcccg gcgtgtcttc cccatcgtcg cgcggctccc ggagctcctg
    51721 cgccggctca gggagctgga ggagaaggtg gcggcgctcg aggggcggga cggaccgggc
    51781 acgtgaggga gcggcgcgtc gtcgtcaccg gcctcggcct cctgacaccc ctcggcaccg
    51841 gcctcggggc gacgcggctg gcgctcgccg agggccggtg cgccatcgct cccgccacgc
    51901 tcttcgacgc gagcccgttc gcgtcgccgc tcgccggcga ggtgcgcggc ctcgaccccc
    51961 gcgcgtactt ccgcgttccc aaggcgctga agctctgcga ccgcaggacg cggttcgccg
    52021 tctgcgcggc cgcgatggcg ctcgaagacg ccggcttccc cgcggggccg tgcgacggcc
    52081 tcggcgtctc gatcggaacg agcggctccg acctcggcgc cgaggagctc tccgcggccc
    52141 tcgcgcgttc ggaggacccg gccctctcgg cccgcgacac cgacgcgttc ggcgaggcgg
    52201 ccctcgcggg cctcaaccct ctctggctcc tcgtccacct ccccaacatg accagcgccc
    52261 acgtggcgat ccagcgggag gcgcgcggac cgaacaccac cgtgatgacg gggtgggccg
    52321 cgggtctctc ggcgatcgtc gagggggccg cgtggatcgc cgaagggcgc gccgacgccg
    52381 tcctcgccgg tggtgccgac tccgccgtcc acccgttcgc gttcgccgcg ctcgagcaga
    52441 agggcctcct ctcgggcccc tctccgctcg ttcccgccga aggggcggcc gtcgtccttt
    52501 tcgaggagcg ggaggccgcg ctcgcgcgcg gggcccgcct cctcggcgag gtgggtgccc
    52561 acgccctgtc cgcggggacc cggctcgccg cgctcgcggc cgacgcgacg cccgccccct
    52621 cccgccagct cgagcgcctc gccgggaatc tcctcgcggc gcacgcgccg gccgcactcg
    52681 tcctctccct cgccgaaggc ctcgccggcc cggtcgcggc cgtcgccatg ggacctgccg
    52741 gagaagcctc ggccttactg gtcctgccgc ccgatgaggc ggcccggagt gccgcatgaa
    52801 cccgtcccct tcggaaatcg aatcccgcgt cctctcgatc gtcgccgacg ccctcgacca
    52861 gcccgccgag cgggtgactc tcgacgcgtc cctcgtcgac gacctgggcg ccgagtcgat
    52921 cgacttcctc gacatcgtct tccgcctcga gagcgacttc ggcctgaaga tcccagagga
    52981 ggagatctgg caggggtcgt tcgacggcgt cggtgacgac cccgacgcgc tcgccgagcg
    53041 gctcgcgcag ctgaaagtcc gcaggcccgg gttccgctgg gatcgcgtcc cggaccgccc
    53101 cacgcgctcg gacctccccc gcctcatcac cgtcgggacc gttgtggagt acctggagaa
    53161 gcgcctctcg ggaggagccg cgtcgccgga atgagaacgc gccgacgcgt cgtcgtgacc
    53221 ggggccggac tcgtcgcgcc gggcgcggac ggtgcggccg gcctctggga gaacctcctc
    53281 gccggacggt ccgccatcgg cccgatccgg cgcttcgacg cgtcctccta cccgacgcgc
    53341 atcgccgccg aggcgctcgc ccccgcaggg gccccggcga gcgtgctcgg cgaggagttc
    53401 ggcgggcgcg ggaggatctg ggacttcgcg gccgaagcgg cctcgcaggc gctttccgcc
    53461 gccggcctcg cgccgcgagg cccctgggcc gcttccgcgg gcctttccct cgcgacgggc
    53521 atggggacct acggtcacgc cgaggtcttc ggagccgccg ccgcgggagc ggcccgggga
    53581 ggcttcgacg ggaaggcgtt cgcggaggag ctgcgcctcg gcctcctccc tcacgcggcc
    53641 gaccggcaga gcccgggcgc gctcgccgcg cacctcgcgc gcgcggcggg gatcggcggc
    53701 cctctcctcg cgtcgatgac cgcctgcgcg ggcgggaccc aggcggtcgg cgacgccttt
    53761 cgctggattc gccgcggcga agcggacgtt gtcctcgcgg gcgcgtccga ctccgagatc
    53821 tacccgatgg gcctcgcgtc gttctgcctg ctcggcgctc tctcccgtcg caacgacgcg
    53881 cccgaacgcg ccagccgccc gttcgacggc gcgcgcgacg gcttcgtcct cggagagggg
    53941 gcggggttcc tcgtcctcga ggagcgtgag cacgcccgcg cgcgaggtgc ggacgtcctc
    54001 gccgaggtgg caggattcgg ctccgcgtgc gattcctacc gcgtgacgga cccccacccg
    54061 gagggcctcg gggccgccct cgcgatgcgc cgcgccctcg acgacgccgg gctctcgccc
    54121 gacgacgtcg actacgtcaa cgcgcacggg acctcgacgc cgggaggcga ccgggtggag
    54181 gtccgggcga tgaaggcggt cttcggttcg cggctcgacg gcgtcgccgt cagctcgacg
    54241 aaatcgatga tcggccacct cacggtggcc gcgggcgccg tggaggcgat cgtcaccgcg
    54301 ctctcgctcc gggacggacg cgtccacccc acgctcaacc aggagcttcg cgaccccgag
    54361 tgcgagatcg acaccgtccc ggagggagcc cggcgcgtcg acctccgggc ggcgctctcg
    54421 aactccttcg cgttcggcgg ccagacggcc tgcctcgcgt tcgtgcgggc gtgaagatcc
    54481 gccgctcgcc accgcgctcg ctcgcccgcg cgctcggccg gatctacggc ctcctccacc
    54541 ggacgcttcg cctggaggga atcctccccg acgggtcccg cgtgaccccc gccacctacc
    54601 cgttcggccc cgaggtcttc gccatctgcg agagggacgc tctcgccctc gcgggcgtcc
    54661 tcggtcgggc gcgcttcacg actttggtcg cacccggccg agacggcgac tgggcgacgg
    54721 aggtcgtcac gtccctcggg ggccgggtcg tccgaggcgc cactctcagg ggcggagcgc
    54781 aggcgctcgc gcagctcctc cacggtcttc ccggagacga cgggcccctc gccctcgtcg
    54841 tcgacgggcc cctcggcccg tccggcgtcg ccaagggcgg agccgtcgtc tgcgcggcgc
    54901 ggacgggtcg cccgctccgc cccgtgggag cggcggcccg gcgaggcctc gtcctgaagc
    54961 gaagctggtc gaggatctgg ctcccgctcc ccttctcgcg cgtcgtcgtc gtcgtcggcg
    55021 agcccctgcc cgttccggcc gggatgggcc gcacggaccg ggagcgcctc gccacggagc
    55081 tcaccacgcg tctcgcggcg gcacgaagcc gtgccctgga ggaggtggcc ggggcatgag
    55141 gcggttcctg ggatactggc tccttctctc ccccgcctat ctcgtcgccg cggccctctc
    55201 ttcgtggctc ctcctgaacc tcgtcgacgt gacctacgga gccttcgcgg cgtggctcct
    55261 cgtccccgtc gcgcaggccc tcgccctcgc ggcgacctcg cggcagatgc ccctcctccc
    55321 ggccggccgc cctccgctcc tcgcgttcgt gacgctcgcg tccggcctcg ccgtcgtcgc
    55381 cgcgggactc gcgcggccgc tcgacgcgag gtggggcttc gccggttccg gagacctcca
    55441 gcgtctcgct ccgcgcgtcc tcgcgatcct cgccggggcc gcgttcctcg cgtcctgtgc
    55501 ccgcctcagg aaggggcgtc ccgccgtcgc gtcgctcggc gcgctcctgc tcctcctcgg
    55561 gctcgacgcc gtgcggccgt acctcgcgac ggtccccggc gccctcctcc cgaggcttgg
    55621 gccgttcctc ggcggcctcg tcacctgggg agtcctcgtc gtcctcgtct ttgctgcagc
    55681 gctcacggcg cagcgggcgc tcgaggagac gagcgcctgg gcggcccgcc tcctgggcgc
    55741 ctcgctcggc gtgcttttcg gcgccctcca cggcgcgctc ctccagctct tcctccaccc
    55801 ctggctcgag aagccctggt cgatcctgat ccccgccgcc gtctccgtcg ccgccgcgct
    55861 cgcggcggcc gccggcctgg cggccctcgt cggccggcgg gagagcgcat gacgagaggc
    55921 gctcccgtcc gagccctcgt cctggcgcgc gacctcttcc tcacgctcgt ggccctgccg
    55981 ttcctcgtcc ccctgcgcct ccttccgtgg aatgccgccg gccgcctcgg gagcctctac
    56041 ggactggcgg cctgggccgt cctggggacg tcgcggcggg ccggtctcgt caacctccgg
    56101 cgcgcctacg gaccgtcgat gtcgcgcgcc gaggcgggcc gcgccgtccg agccgtcttc
    56161 gcgagcctcg ggcggagcct cgcggaggga atccgcttct cggccctgcc cggccgggcc
    56221 gacggctcct ggaggacgct ctacgatgcc gaggatcccg ggctcgaggc gcggatcctc
    56281 gccgacccgc gcccgaaggt cttcgtcacg gggcacctcg gctcctggga ggtcgcgatg
    56341 cagatgctcg acctggcgct cgaagggcgc ggcgcggcgg tggcccggag cgtcgacaac
    56401 cccttcctcg acgcgcttct ccgccgcgtg cggcgcggcg acggcgccct catcgaaaag
    56461 agaggcgcgg tcgttcccgc tctcgccgcg ctccgctcgg gcacgagcgt ggcggtcctc
    56521 ctcgacgaga acggcggccg ctccggtccg ttccctctct tcttcgggcg ccccgcctcg
    56581 acccgcaaga cggcggcgct cctctccgcg cgcaccggcg cgccgatcgt cgtcggcgcc
    56641 gccgtccgtc gcggggagcg gttcctcttc cgcctcgcct tcttcgagcc tcccggaacc
    56701 ggcgtcccga ccccgagcga cgtcctcgag acgacggagc ggatcgtccg ggtctgggag
    56761 gcgtgggtcc gggaggaccc cctgcagtgg cggtggatcc actggcgctg gaagacccgc
    56821 cccggcggaa gcgaggagac ctacacccgg cgcgacgtga acgcggcttt cgcggccgac
    56881 ggcccggccg ccagggaggt ctccgcatga ccacgccctt ccccgacctt cgcggccgcg
    56941 tcgccgtcgt ctccggcggg acccgcggcc tcggccgcgc gatctccctc gcgctcgccg
    57001 cttccggcgc ccgcctcgtc ctcggctacc gcaccgacgc cgccgcggcg gaggaagccg
    57061 cccgcgcctg ccaggacctc ggcgccgagg tccggaccgt cgcggcgaat ctcgtccacc
    57121 ccgaggaagc gagggggctg gtcgcggcgg ccggcgaccg ggtcgacgtt ctcgtccatt
    57181 ccgccgccct cggctccttc aagccgctcc tcgacgtgaa gcccgcgcag tgggacctga
    57241 cgctcgccgt gaacgcccgg gcgttcctcc tcctcgcgcg cgaggcgtcg gagcggatgc
    57301 cggacggagg gaggctcgtc gccctctcga gcctcggcgg gtcgcgcttc ataccggagt
    57361 acggcgcgat cggtccgtcg aaggccgccc tcgaggccgt cgtccggact ctcgccgtcg
    57421 agctgggtcc gcgcgggatt ctcgtcaacg ccgtctcggc gggtctcgtg ccgacggcat
    57481 cggtgaagct ccacccgcgg tacgaggagc tcgccgccca cgcgcgcgcc gcctctccgc
    57541 tcggccgcct cgggacgccg gaggacgtgg cctcggcggt cctcctcctc ctctcgccgc
    57601 tcgccgggtg gatcacgggg cagacgctcg tcgtggatgg cggagccagc ctcgcgacct
    57661 gaacagacac gacgggtcgg accgtgcctt cggcgatgcg gcgacgctcg tcggtcgtct
    57721 cgaaccgggg cggacgaccc tgatccggtc ggcccgctcc ttgcttcggg ggttggcggc
    57781 cggtaacctc cggagcctct atggaagccc gcgactacaa ggccacgctc caactcccca
    57841 cgacctcctt cccgatgaag gcggacctcc cgaagcggga gccggagcgg ctgcgccggt
    57901 ggctcgactc cggcctctac gagaaggtcc tcgccgcgcg acgggccgcc ggcgctccgc
    57961 gctacatcct gcacgacggg ccgccctacg cgaacggcca catccacatc gggaccgcgc
    58021 tcaacaagat cctgaaggac gtcgtcgtcc ggtcgaagac gctcgccggc ttcgaggcgc
    58081 cctacgtccc cggctgggac tgccacggcc tcccgatcga gctgaaggtc gacaaggagc
    58141 tcggctcgaa gaagcgcgag atggacccgg tcacgttccg gaaggcctgc cgcgagtacg
    58201 cgcagaagtg ggtcgtggcc cagcgagacg acttccagcg cctcggcatc ctcggcgcct
    58261 gggaggcgcc ctaccggacg atggactacg gctaccaggc gacgatcgcc cggtcgttcg
    58321 gcgagttcct cgcgaagggg ctcgtgacgt tcggcttcaa ggcggtcctc tggtgcgtca
    58381 actgcaagac cgccctcgcc gaggccgagg tggagtacga ggaccggaag gacccctcga
    58441 tccacgtcgc gttcccggtg ccggtcaccg aggcgcttcg agccctctgg ggcgaggccc
    58501 tcgcggggga ccccgagctc ttcgccgtca tctggacgac gaccccctgg accctcccgg
    58561 cgaatcgcgc catctgcctc gggcccgaga tcccgtacgt cctcgcgcgg agggcttccg
    58621 agccggcgaa gctctacgtc cttgccgagc cgctcgtcga agcgacggcg aaggccctcg
    58681 gctggagcga cctcgccgcg gtccccggca cgacccgcaa gggggccgag gtcgagatgg
    58741 cccggccgtc gtaccggcgc ccgttcgacc tcgacggcat ctccttcggg ttcctcctcg
    58801 gcgagcacgt ctcgacggac gacggcaccg gcctcgtcca cacggcgccg ggccacgggc
    58861 gggaggacca cgacgtcgtc aagcggagcg gcttcccggt cgacgacccg gccctctgcc
    58921 ccgtcgacga ggcgggcttc tacgaccggc acgtccccga gccgctccgc ggcaagcggg
    58981 tcgtcgcggc gaagagcccc gacctcgacg cgaaccgggc cgtcctcgcg atgctcgagc
    59041 agggagacct gtcgggcgcc cgcctcctcg gccggaccga cgtcgcgcac agctacccgc
    59101 actgctggcg ctgcaagagc cccgtcgtct tccggtcgac gcaccagtgg ttcatcgacc
    59161 tcggcgcgct ccgcgagaag gccctctccg agatccgcga gcgggtcgag tgggtccccg
    59221 cgttcggcgt aaaccggatc ggcgcgatgg tcgagaaccg cctggagtgg acgatctcgc
    59281 ggcagcgcct ctggggctcg cccatcacga tcctccggcc gaaggagccg aaggacggga
    59341 tgccggagta ctacccctcg gccgacgacc cggcggagct gaagctcttc ttcgacaacc
    59401 tcgtcgcgat cttcgagaag cacggcgccg acgcgtggtg ggacgacgag gccttccccg
    59461 ccgacgcctt cgtcccgccc gcctcgaggt ggaaggggac ggccttcgag aaggtgaagg
    59521 acatcctcga cgtctggttc gactcgggcg tctcgcacgc ggccgtcctc ggcacgtcgg
    59581 gctacggcat cgcgtcgccc tacgccgacc cgaagaggcc ccacgtcctc tacctcgagg
    59641 ggcacgacca gcaccgcggc tggttccagt cgtcgctcct caccgccgtg gcgctcctcg
    59701 gccgcgcccc ctacgagacc gtcgtcacgc acggcttcgt cgtggcgggc gacggccgga
    59761 agatgtcgaa gtccgacggc aacaccgtcg agccggacgc cgtcatcgag aagcacggcg
    59821 ccgacgtcct ccgcctctgg gtcgcctcgt ccgactacac cgacgacatc cgcctcaacc
    59881 aggagatcct gacgcggacg gcggaggcgt accggaaggt ccggaacacg gcccggttcc
    59941 tcctctcgaa cctcttcgac ttcgaccccg tgacgaacgc gctcgcgccc gaggcgctcg
    60001 agccggtcga ccgtctcctc gtcgcccgcg cggccctcct cgtcgccgag tgccggaagg
    60061 cgtacgaccg cttcgagttc cacaccgtgg cgcgccgcct ccgcgagttc gcgacgacag
    60121 acctctcgtc cctctggtgc gacgtgagga aggacgccct cttcgtcctc gggaaggacg
    60181 acccggcccg acggtccgcg cagactgccg cataccgcct cgccgaggcg ctcgccctcc
    60241 tcctccaccc gatctgcccg ttcacggcgg aagagatctg ggaggcgctg ccgggcaacg
    60301 agggagggac tcccgcgctc gcgacctggg acgacctccg cctcgcgacg ctctccgacg
    60361 aggcgcacgc ggcctggggc cggctcctcg acgtccgcgc cgcgttcgtc gccgccctcg
    60421 agccgctccg gcgcgacggc gtcgtcggca cgagcgctca ggcgcgggcc gagatcgaga
    60481 ggactcccgc cctggaggcc gacctcgccg tcgtcgggct cgacgaggcg cgcttcgccg
    60541 agctcctgat cgtccccgag gtcgtcttca cgccgacagc gggcggcacg ccgctcccgg
    60601 tcgtctcggc gcaggccgcc gaggggacga agtgcccgcg ctgctggcag gtgcggcgcg
    60661 acggcgacgc gtcgggcctc tgcgcccgct gccggcggat cctcgcggcg gcctcggcgt
    60721 gagccccgtc cgcctccggg ccctctacct cgccctcgcg gccttcgtcg tcctcctcga
    60781 ccaggcgacg aagaagatgg tccaggcctg ggtcccgctc tacgaccgga tcgtcgtcat
    60841 accgggcttc ttcgacatca cccacgtcga gaacaccggc gccgcgttcg gcctcttcgc
    60901 gggagcgacc tccccttacc ggccgcttct cctgaacacc gtcgcgttcg cggtcttcct
    60961 cttcgtcctc gtctacgcgt tccggagccc cgtccactgg aagaggctgc agctcgccct
    61021 tgccggcatc ctcggtggcg cgatcgggaa cctcatcgac cgggtcggcg ccggagcggt
    61081 caccgatttc ctccgcgtcc acgccgccga ccggtgggag tggccctcct tcaacgtcgc
    61141 cgacagcgcc atcaccgtgg gcgtcgtcct cctcgctctc gacatctgga agaacccgga
    61201 gagcgaggag acgaagccgg agtcctgatg caccccgagc tcctccacct cggcccgatc
    61261 gtcctcccct cctacggagc cgccctcggg ctcgcattcg tcctcggcat cctcctcctt
    61321 cgccgccggg cacccgagta cggcatcgac cgggagaccg ccgtcgacat ggggatctgg
    61381 ctcatcctct ccggactcct cggcgcgaag ctcctcctcc tcgtcgtcga gggcccggcc
    61441 tactacctcg gctccttccg aggcctcctc gagctcttcc gcgccggcgg cgtcttctac
    61501 ggcggcctgc tcggcgcgct cgtcgcggcg gtcttcttcg tgaaggtgaa gaaggtgccg
    61561 ttcctcgcgt tcgccgacgc cgccgccccg gccgtcgccc tcggacaggc catcggccgc
    61621 atcggctgcc tcgccgccgg ctgctgctgg ggcgggcagt gcacgaagcc ctgggcgatc
    61681 accttcagcg acccgaaggc gggcgagaac gtcggcgttc cgatcggcgt ccccctgcac
    61741 ccgacgcaga tctacgaggc gctcgggctc ctggcgctcg tcgggctcgt cctctggttc
    61801 ggcaaggggc gcgtcccggg ccgcaccttc tcggtctacc tcgtcggagc ggcgatcctg
    61861 cgcttcgtcg tcgagctctt ccgcggcgac ccgcggggga ccgtcccggg cacggacctc
    61921 tctacgtcgc aggggatcgc gatcgcgctc ttcgtcctcg gcgtcgggat cgtcctcgcc
    61981 ggccggaaga aggccgacgc accggccgag gcgtgagcgg cgacggcgag cgccctcgcc
    62041 ggagcttcac cgccgaccgc ggcgacgacc gcgagcgcct cgaccgcgtc ctcctccgcc
    62101 acctcgccga cctccctgag gcctcgcgga cgaaggtcca ggagtggatc gaggcggggc
    62161 tcgtcctcgt caacgggcgt cccgtcacga agaccgcggc aaaggtcgcc gccggcgacg
    62221 ccgtcgacac cgtcctcccg cctcccccgc cgccgaagcc ggagctgatc ccgcaggaga
    62281 tcccgctctc gatcctcttc gaggacgacc acctcctcgt cctcgacaag ccaccgggcc
    62341 tcgtcgtcca ccccgcggtg ggccaccggg acggcaccct cgtgaacgcc ctcctcttcc
    62401 actcgaagga ctgggccggc gaggcggacc gcccgggcct cgtccaccgg ctggacaagg
    62461 acacctcagg agtcctcgtc gtcgcaaaga cggaggccgc ccactccggg atggcgaagg
    62521 cgatgaaggc gcggactctc gtgaaggaat acctcgccgt cgtctacggc cggacggccg
    62581 tcgcgaaggg gaaggtcgac ctgaagatcc tccgcgaccc tcacgaccgc aaacgcatga
    62641 ccacctcgaa gaccgagggg cgcgagtcga cgacgctcta cgagcgtctc gccgagtcga
    62701 cgggcgagcg agcggggctc tcgctcctcc tctgcacgct cgtcaccggg cggacgcacc
    62761 agatccgcgt ccacctcaag gcgatccagc acccgatcgt cggcgacgcc acctacggct
    62821 cgccgcgctg gaaagggatc gccgaccccg cgctgcaggc ggcctgccgc gacttcccgc
    62881 gccaggcgct ccacgcccgc cgtctcgcgt tcgaccaccc ggtcacgcgg gcgcgcgtcg
    62941 acacgatcgc gccgattccg tcggacatcg cggcgctgct cgaagcggca gggctttccg
    63001 ccccggcctg actagccgag cgccgccacc cgcgcccact ccgtccgcga gcggtcgacg
    63061 cagtcctcga tcgagagtcc ggcgaaccag ttcccgagga cacagagcct ctttcccgcc
    63121 gagaggcggt cgatctccgc cacgatcgcc tcgtgcccca gggcaggcga cgggaggaca
    63181 gttcgcttct ccgcgacctc ctcgaggtcc gcgcgcgaca gcccgagcac tcgagccgct
    63241 cgctcgagcc tctcattcgc cgcgagcccg ggcttgaagt ggaacgagaa cgcccgccac
    63301 tcggcgtcgg gaacggagtc gcgcgtcacg atcgagtgga aggaatcggc cttcggcacg
    63361 aggaacatcg acaccggaat gcgcgacacc ttcgccgaac ggacggcgaa gccgagcgtc
    63421 tcgacgaccg cctccttcac cgccgcggcc gcaccggcca gctcgggtgt ggcgccgccg
    63481 aggaacgctg cggcggctcc ggggggcgtc gccacggcga cgatgcgcgc ctcgtggcat
    63541 gcaccgtccg acgaggtcac gacccagccg tcgccgatgg cggcgacgga cgtcgccgcc
    63601 atccccgtcg tcagggtcac gttcggcgtc gtcgcgagcg cctcggccgc cgtctggagc
    63661 ccgccggcca gggtgaagct ccgcggaaag tcctgccgtc gcgtgtcgcg catcttgaag
    63721 agcatcccgg ccgggaagcc gtcggcgctc tgggacggca cggccgagag catcggtccg
    63781 aggacgcggg cgtagttccg cttccccacg atgcggccgt agaactccgc gacggtcctc
    63841 ccgtccttct tcgcgccgat caaccgtggg aggctcacgg ccgcttcggg ccacgagaag
    63901 agccgcagca ggacgcccag gttcgagccg ggaacgaggc gatccccgtc gaggaagcgc
    63961 aggtgcgtct tcgcgcgccc gacgacggaa tcccggaggc cgcacgcctc gaggacccgc
    64021 gtgaaaccga cgtaggagtc gtagcaggtg tgggccccca gctcgaacca gtagccgctt
    64081 cccgcgcgcc gcgtcgcgag gcagccgccg gcgcgatcct cctgctcgag aacgaggacc
    64141 ttccgtccgg cccgtgcggc ttccgccgcg aacgtcagac cgctgatccc cccgccgaca
    64201 acgacgacgt cgtgcttgct catgcggtgg cgaggttcgc acgaacgagg taccggcgcg
    64261 aggccggcct ctcagcgaga ggaacacgtt tcgtccgggg caagtcgtgt ccgatcaagc
    64321 ggttggtcgg aaatctgcga tgtcatatca tttgaagaaa ggagcccgga atgaagaacg
    64381 tcctcgctcg cacggcgatt gccgcgtccg tcttcctttg cctcgggagc ccgcgcagcg
    64441 cgttcgcgca ggacgatccg caggtcctcg aaggcgaggc ggcgttcaag catccctcca
    64501 cgcaggtcgt cctgaaggcc gccgagcatt tccgcgccgg gaagatcgac gcggggatgg
    64561 cgctctacac cgcgtccgag caggccgact ggaagaagtc gagcgagaaa gcggagctct
    64621 ccgcccggcg gaaggaacgc gcgcccgacc cgaaggcctt cgccgagggg atccggaagg
    64681 gcggaacgct gacgctctac gccaccgagg gccagctgcg cgccccgctc ccgaaccgcg
    64741 cgatggggat cgcctacctc gccctcgaga acggcacgtg gcgcctcacg ggcggcccga
    64801 tggccatcga gaacgcgcag gacccggcaa aagaggagcg cttccacggc gacgagatgc
    64861 gcgcgcatcc cgtctacgcc ctcgcgctga agtacgccga gatgctccac gcgaagaagg
    64921 acgactcgtt catgcagctc gccacgaaga gggcccggga gaagtgggcg gcggagcccg
    64981 cgagcgagcg gtccgagagc gcggcctacc gccgccgcac gattccgaag aaggccgacc
    65041 tggccgcgag cctgaaggac ggcggcttca tcgtcgtcga ggacgacgtc cgcgcctcgc
    65101 tcgtcctggt cagcgtcacc cagagctcgc cggagccggg gaccgtgtcg tccacctcct
    65161 ccacgctctc cctcggcttc gagctcgagg acggcgagtg gagagtggcg aactgactgg
    65221 ttggtccgac gtcgcggctc tcgccggccc ccgagctggc gagagccgcg acgccctccg
    65281 gaactccgta actccgttac tccaggcgtc cagggtgttc gactagaact tcgtgacctt
    65341 gtcgaccttc ccgttcagct tgaactggac cattccgcca tccttcacgt ggacgatcca
    65401 gccgtagctc ttgcagaaga cgccggcgct cagctggaca actgtcaccg ctccgcccgg
    65461 cgcaccgagc agggcgggcg cctgcttcac cagccccggc accacggaga agtcgacctt
    65521 cgcgagggcg atcttcttct cgcagtcgtc ctcgctcccg tcgttcgggc ccgtcacggc
    65581 cccaccctcg tacttcagct ggcagcgccc cgaccccgct tcggcgcagg ccgtcgtggc
    65641 gagcgcgaac ccggggtaga tcgcgagctc gtagacctcc ggagcgcggc cgggcagcac
    65701 ggcctgcagc tgggagaggt cgtcgaagag gctccccttc tgcttcggcc gcgtcgcgtc
    65761 ctcgattgcg acgacctcgt cctttgcgtc cggctgcacg cggaagagag cgtttcgggc
    65821 cgcctgcgcg atgaacccgt ccgggtcgcg gagcgattcc cggaggagcg ggatcgccgg
    65881 cttcgccgcc tcccgcatct cgccgagagc cgacagagac gcggagcgga ccttcggcca
    65941 cttgtcctcc ttcgccgcct tcatgagcgc cggcaccgcc ggcttcgcgc acttcggtcc
    66001 gatgttgccg agcgcctccg cggccgagac gcgcacctcg ttgtcgcggt cgcccgcgag
    66061 gagctccagg agccggggaa ccgccgggag cgcggcgtcc ttctggtagc cgatcgagcg
    66121 ggcagcgctc gcgcgctcgc ttccgctcga cgaggcgagc cgggcgacga gctccgagac
    66181 gtctttcggc gccttctgcg cgagcgcagc gggagccagg agcatcagac acagggccgt
    66241 cacgaggtga cgtcgcatgg ggcctcctct tcggggacga accttcgttc ctggtttggc
    66301 gggatggtag cgcgactcga agcgccgacc cgccgatcgc gggcgcgggc cacccgctgg
    66361 tgtgaactcc tgtcacacga aagggccgga aacgaacctt tttccgggtg acccgtagga
    66421 gcggacgacc cgctcggcag ttccggacca tccggtctcc gagcgtggcg atcctccggc
    66481 ccagggaggt ggttcgacaa ggtcgcgggt gcgacgaaac gacacagaat ccggcggacc
    66541 cgaaagaacc cggcagcgcg tgtgccgact tccgctcggc ccgtgagggg tccgctccga
    66601 cgccacagcg caacgtgccc aaaggagacg cccaaatgaa gtcatccctc tgcaaggtct
    66661 tctctctcgc cgccctcgtc ctgctcgccg cgggctccgc ggtcgcggac gacgccgccg
    66721 tcttccggct cgaccaggcc gtccaggcgg gaaccctcac gctccccgcc ggcgtctacg
    66781 agttccgcgt ctccgaccgc ggcgtcgtct ccgtcttcga cgacgagacg tccgccatcg
    66841 tcggcgtcac gatcgcgcgc cgcgaccccc tcgatctcgc gcatcgctcc gcgtcggcga
    66901 cgctgacgca cgaccgggcc gtccgctcga tctcgatcgg cgacttccgc tacagcttcc
    66961 gcgcggcgcc cgcgccggcc gggatggcgg ccctccagcg gccgacgacg gtcgtcgcgc
    67021 tcgctcgcta ggacgggccc ggggccagaa gccccgagcg ccttcccccg acccctccgt
    67081 ccgcgaccga agggccggac cgggggtcgg ggggctaatc ttcccgcgtg ccccgtgccc
    67141 cgttgccccg cgtcgtcgtc cacctcgaca tggacgcctt ctacgcctcg gtcgaggtgc
    67201 gcgaagaccc gtcgctccgt ggccgccccc tcatcatcgg gcacaggggt cgtcgcggcg
    67261 tcgtctccac ttgctcgtac gaggcacggc gattcggcgt ccgctcggcg atgccgtcgg
    67321 tcacggcgga gaggctctgc cccgacgcgg tgtggcttcc cgggcgaatg tccctctacg
    67381 tcgacgtctc gcgccggatc cgcgaggtcc tcgacggctt ctcccctctc gtcgagccgc
    67441 tctccatcga cgaggcgttc ctcgacctga ccggcatcgc ccacgacctc gcagacggac
    67501 gccggatcgc cgcggagatc aagcgcgaga tccgcgatcg cgagcggctc acgggctcgg
    67561 ccggagtggc tccgaacaag ttcctcgcga aggtggcgtc cgacctcgag aagccggacg
    67621 ggctcgtcgt cattccgctc gaggaggtcc cgacccgact ctggccgctc cccgtcgagc
    67681 gtctctgggg cgtcggcccg aagacggccg aacgcctccg cgagcaggga ctcgtgagga
    67741 tcgtcgacct cgtgaacgca ccttccggac gtctcgcccg catcctcggt gaagggagcg
    67801 cggagcacct cctcgccctc gcccaagggc gcgacgaccg cgccgtgcag ccgggccgcg
    67861 aggcgaagtc gatctccgag gaacggacct acggcgagga cctcacgcgc cccgccgaca
    67921 tcgacaaggc cctcctcgcg cgggccgatg gcgtcgcccg cgagctgcgc cacgagggcc
    67981 tcgtcgcgcg gaccgtccac ctcaaggtcc gcaccggcga cttcgagacc gtcacgcgcg
    68041 cgaagacgct ctccgagccg acggacctcg ccgaggtcgt cctcgaagcg gcgcgggagc
    68101 tcttccggga gcggatcgcg ctgaaggggc gcggcatccg gctcctcggc gtcggcgtca
    68161 gcggcctcga ggctcccggt gtcggccagc cctcgctctt ctccgacgag cggcaggaga
    68221 agctccgtgc cctcgcgcgg accgccgacg agatccgcga gaagttcggg aaagccgccc
    68281 tcgcgagggc acgcctcctc gcgacaccga acacgaccgg gaccgacgcc gacggaagcg
    68341 aggacgacgc cgccgaggcc tcgagccttc caagcgtcga ctgaccggcg ccgccgtcca
    68401 cagtcggctc gcgactgcag cgcgcgacgt gaactccgcc attcgcggca cgagcacgag
    68461 gggcgtctcc ggcgggccac cgttggagac tgaggggcgg acgatggcaa acgtttacgt
    68521 ccgtgccgag ggtcgaccgg aacgccctgg tcgcgctcct ccccggctcc cccacggctc
    68581 cgcgcgcaag cgagggatcc gtcgagcacc tctgcccggc ctgcctccgc gtcgtcccgg
    68641 ggccggtcgg gtcggccgag cgctgcccga cccggcctgt cgcatcccgt tccgcagccc
    68701 gaagtgggcg acctggctct cggccctcgt ccctggcgtc ggagacatct acctccggca
    68761 ctttctcctc gggtccctcg agttcgtcgg gtcgatcgcc gtcctctgct tcgccctctt
    68821 cgcgatcgtc gccgccgtcg ccacacgcga ctccgaggtc ctcctcgtcg cggccgtcct
    68881 cggcgtcctc ttcatcctcc tcccacggct cttcgacttc ttcctcacgc tccacatggg
    68941 acgaaagggg atcgtcccgc tctccctgac tccggctccg cccggagtcg aggacgggtc
    69001 ccccatcggc ccgtcccggg gagcctcact cccggccttc cccacctgga cctggctcct
    69061 cttcgccgcc ggagcgctgg ccgtcggcgc caccgcctcg ccttcgctcg ccgaggcccg
    69121 cgtcctcgaa gcctgccgcc tcgccgagac cgggaagctc gccgaggcga ggaagctcta
    69181 tgaggcgtcg gccgccgcga aaccggtccc ccttccgacc gcgggccctt cgccctcgct
    69241 ctctgggaag ggggcgatct cgacggcgga gacctcctcg tgaccgacct cgggccgatc
    69301 gaccgtgagg tcgccgaccg actcgacgcc ttcgtcgcga atcacgaggc cgcgctgaac
    69361 gacc
//
LOCUS       DAOONL010000010        65956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_262_length_65956_cov_20.241119, whole genome shotgun sequence.
ACCESSION   DAOONL010000010 DAOONL010000000
VERSION     DAOONL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 65956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65956
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_65956_cov_20.241119"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2837
                     /locus_tag="PLP50_02965"
     CDS             <1..2837
                     /locus_tag="PLP50_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPA50541.1"
                     /translation="ETKLPIPYASRAVFSPDGKTIAYTPNRPAHLQWKRYRGGTVSRI
                     WLYDVATHAIVKVPQPEGRCNDLNPAWVGDTLWFTSDRDGEFNLHTFDAKARSVAKVT
                     AHEDFPVLAAGAARGKVVYEQAGLLHLLDPATKKAAPLPITVNADLLETRPRWAKGAE
                     WVRDASLSPSGARVALEFRGEIVTVPAEKGDPRYLTSTTGAHERGPAWSPDGRSIAYV
                     SDEGGEYRLVVAAQDGKGTPKKIAVPGSGFYDRLAWSPDSRKIALTDNSWTLWWVDVA
                     SGKATKVVSERLYAPQKTLKASWSPDSRWLAYTLAGATYIRTLHVHDTVDGKSFPLTD
                     GLSDVSDPVFDKGGKLIWFLASTDAGPVRNWFSLINQDADVTRAIYVATLKAGTPSPL
                     AKESDEEAGEGAKKDDAAKAADGKGGKDAKKDEAKKEEKAPEPVVIDRDGFDLRIVDV
                     PVKAADITALSSPNAGEVWFLREADGKTALQKWDTKSRKAETIAAEVDDYEVSFDGKK
                     LLTRVKDSFEVGPAGKKGEGKPGDGGKLKLDAVQVRVDPRAEWPQILEEVWRINRDYF
                     YDPGMHGRDWKAIKAKYESFLPHLSSRADLTRLIQWMCSELAVGHHRTFGGDRLDEAT
                     PVPGGLLGADYEVANGRYRFRKVFGGLNWNPELRAPLSEPGVEVKAGEYLLAVNGKEL
                     APPENLFSRFEATAGKLVEITVGPNADGKGLRTVNVVPVANEAALRNRDWVEGNLAKV
                     EKATGGRVAYVYVPNTAGLGHTYFKRYFYPQAWKDAIIVDERFNGGGSVADYYIEALR
                     REPIAWWTFRYGEDMKTPSASIQGPKVMLIDETAGSGGDLLPWMFRKFKVGPLVGQRT
                     WGGLVGILGFPVLMDGAFITAPNLAFWAPEEGFGVENVGVPPDVEVEQTPADVIAGRD
                     PQLEKAIEIVMEQLKKSPPAAPKRPPFPIR"
     gene            2876..3610
                     /locus_tag="PLP50_02970"
     CDS             2876..3610
                     /locus_tag="PLP50_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50542.1"
                     /translation="MRRAVLSAGFALALLAPLALASRPARADALADLRGALDRAPAGE
                     TVRVRVAIARTDVEKEKPKGERKGEAVVEHGPTGLSIHLDPAFLPKPGSKAERKKHDE
                     KIVRLEPGEALKLVDPATELRQLLDGASVVSDRPETFEGKSARTVVFRVVPDLDDEAR
                     KAVKRYDDVVTLRLDADGLPSSLERTLDLKAKKLLISFTVSMRESRRFTRVAGRLVTA
                     SAREESHSSGLGQESASTTRWTVTPL"
     gene            3718..4911
                     /locus_tag="PLP50_02975"
     CDS             3718..4911
                     /locus_tag="PLP50_02975"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPA50543.1"
                     /translation="MLEPTIDPAKLDEWARLLVTHSIGGVTPDDRVMIKGERIAWPLM
                     ERIEREVIAAGGLPDVCLVAPNNERGKVWSAAMGRGASEEQLARVPEWHRERYASMTK
                     YIEVLGAESPSSYAGLAREAGAALAKADRPFADLRLSKRWVITLYPTPAYAEIEGLPL
                     EEYVRFVVDASTVDPRPLLEAEKRLEPLFADGKRMEVVTFHPIEKRELTLAMSLAESI
                     PVLSHGLRNVPDGEVFTSPDASSVEGELFLDLPVLNGGVDVSGMHLVFEKGRIVRFSA
                     LEGETQLAAIVGTDDGARRLGEVALGMNPGLTRALKHPLFVEKVGGTLHVAIGASYET
                     CFERDPKAPGARERLEELAARGVLNRSAQHVDVVCDFRPGGCGRRVSIDGRPIVVENG
                     IWVPG"
     gene            4969..5379
                     /locus_tag="PLP50_02980"
     CDS             4969..5379
                     /locus_tag="PLP50_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPA50544.1"
                     /translation="MTPDDGRHDLVTGNREMDEEHALQLQLLRELVQNLESGDGPAAL
                     EVFRRLDGFTNAHFLAEERVMRLHAYPDSEAHRAEHGSFVEELTTLRARLSAGDVDAV
                     HDDADAFARRLPAHIARADHALAEFLRPRRGAVA"
     gene            complement(5392..6555)
                     /locus_tag="PLP50_02985"
     CDS             complement(5392..6555)
                     /locus_tag="PLP50_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA50545.1"
                     /translation="MDSGPVSCEICRELKPVETSLMKFGWEENDRLLPAAYRRLVEVR
                     VGGREDEPRSPRLCPLCGTLYSYESRYEYLANGSEDEEELRRLTGDDARAWLGDAVVA
                     ALPRPLLSPLRETVVNERVVVFSTGGEDLASGWGANAVAILGDGATLLVDPLVAPVYA
                     RELARLLAARGAGPVRWVVTTHHHTDHSVGASVFAEAGAEVIAHAASAARMAAEQLAL
                     LAERRTSPSTRALFFDAVPAAPTRLVTEPTDLSVGGVRATVRPAGPAHTPGDLRIDLP
                     SLGVVVTGDLVFNGYHPNLEHADLDGWKRGLGELLLEPAGTTFVPGHGAPGGREIVEA
                     QLAWFEAAAEEVRRVTVGGLSSDRAVERIRERFPGYLLGAVLPTAVRVLGRAG"
     gene            6639..7847
                     /locus_tag="PLP50_02990"
     CDS             6639..7847
                     /locus_tag="PLP50_02990"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HPA50546.1"
                     /translation="MTLSSALARAGWGVLAGALLLASPLAGQGPDDDYDLVLECRGAL
                     WRATFDDATAAGLDPGAAPVSFDGSGARRTRGDGVVTFRGSEEYQPSHRTRAIPVRYE
                     CVVDTATRKVLSITYEAVDASGAPIAKRPVDLVRDALLLGACRESLEDEVRDDARAGG
                     ASTDGNDLEPDPASVARTEKGKLVELTGRGRARLSKDYEWEAVTFGCRWDPRKEEVTK
                     AWYAPVGARKLGLLTPEKREALDACRRAIRDAVWDDSAARGYRWPRDQVVVELSRRGD
                     FAASGDRVEVKGEGWFKSDARHSQSTPIAFRCAFDANLQAVVDASFEALDASRTPTGE
                     IASGKTGTLVCESFGKVQKVCPASIRGNVRVIRQLSSVACKAYENWIYSLSGITVWGG
                     CRAEFEFDAR"
     gene            complement(7903..9864)
                     /locus_tag="PLP50_02995"
     CDS             complement(7903..9864)
                     /locus_tag="PLP50_02995"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPA50547.1"
                     /translation="MHEALRLLLTNAVLAGLLALAAWVVSRFTKRQAVVHGLFLLALL
                     KLVTPPLVELPLLPAGDAVPRAEEATSSVPSDVRGPAAPEPLAAPRARIVEAARTDAA
                     PGSAGPRSARGALPGAPPADARAVAAPLPWRLALTAIVGLGALAVAALAARRFLRFRR
                     LAEAAEPAPPALVTRAAEIGAALGVRRAPAVCLLPARVPPMLWPASPRPKLLLPRDLL
                     PELGGDELDALLAHELAHVRRRDHWVRFLEIGATALFWWYPVAWWSRRELRRAEERCC
                     DEWVLRAFPASARAYASGILKSLTFLAEGPVALPETASGAGPIRDLESRLKEILMTSP
                     RPRLSRPIRLALGCLAVGALAFFPTVAPPAAAEAAALAGLPDEVPPPPPPAPASPVAA
                     PARPVAVAPRSPAAAPAPPPPAPHAAPMPAPRIAAHPAPAPDVHPVLAGEEPDPELER
                     ERRALDAQRRQLDLSRLDLERRALELEARADRQEQAAEVARLRAEGDAAGAARAEKHG
                     ALRARQADVERRRLELHLRELKLEEESERAAEAGGAERDAEARAREAARAEAELGRQE
                     QLLEAESEKLEREARALEAEARVHALRGAADDLERSLAEQVGELRGELPEAGAEKAAM
                     ERELARLESALAALRGSRPAKEAPRATVR"
     gene            complement(9869..10255)
                     /locus_tag="PLP50_03000"
     CDS             complement(9869..10255)
                     /locus_tag="PLP50_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPA50548.1"
                     /translation="MARTPRDVTDAELEVLRALWDLPEATIRALADRVYPNGGASEYA
                     TVQKLLERLEDKGHVSHRSEGRQNVYAARLGREDLVALRLRETADSLCGGSFTPLLTH
                     LVSGARLSARELRDLRRLVDSLGEQR"
     gene            complement(10384..11457)
                     /locus_tag="PLP50_03005"
     CDS             complement(10384..11457)
                     /locus_tag="PLP50_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50549.1"
                     /translation="MSEAVPAPRSRGSHPWRLAGAGGLEETLVFERRLFDAEGRPLAP
                     DRETVDFAGAGLIPAVRLALAGNVLLGRLSPGDVRAVADELRAAGDGVRDLGGLLEVL
                     GVAARLALDDPMNGVLTGPLQRALAGEGTSLLAALAHVCARPDPRDVLVYVDVFVRFR
                     RGLVALALRLARELPPFPPGVLDGSVEVFAWFRETGARDVDVDVDGRTESVPLPAGLP
                     EPVANAAAGAVAELLASLRRDAERLAHPRRDFSGVARDVGRELLDGLAGLLLNGLSLA
                     RAPRPEQIEARLVPAGGLPTRARHARSVSIAGIPFADALGRFFAVPSTPLTLPRTGRI
                     VVVRRTGGCPFAVAGWSEATPHE"
     gene            complement(11606..13579)
                     /locus_tag="PLP50_03010"
     CDS             complement(11606..13579)
                     /locus_tag="PLP50_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPA50550.1"
                     /translation="MAHYRTVVARTVAAALLMVPALVASRAGAQESSPSAIAAPVLKW
                     QRGGCFSSWCQTGWYSSPAIADLDRDGAPEVVWGSYDVVALDGATGALRWRATNGSRV
                     WPGVAVADLTGDGTLEVVVGRGSDQLTVYDAGGGVVWTRNPFGGGEVRTLAVEDLDTD
                     GVLEVVVGRASGGSTRQLNVYDAAGNVRSGWPARRDGEPGYGWGMYNENVAVADLDGD
                     GDKELIGPTDTHYVTALDGGGNQLPASPIYGAGKVWSQVGVHVDHAVDLRGYANCGVE
                     HRPNWADSAPVIGDLDGDGVREVVIVGNVYNCATNPYTSLYHMPFLFRLDRTRATGAG
                     WDWTVLPTPAAGSGPLSEDYDVIETAVPNPALADLDGDGRKEILYPSYDGRLHAWSLD
                     KTEQGSWPYDVPGSGIRFASEPVVADLDGDGTGEVIFTSWPQKSVGGTGQLHVLSHLG
                     VSLHAVSLPAPHGSATWNGALGAPSLGDVDGDGELEVVVGTVSSGAVAYDLPGTSNAR
                     VLWGTGRGGLRRTGAAPGSAGPPRSSRFYALTPCRLFDTREAAGPLGGPAISAGARRL
                     FPLGGTCGIPPDARAISANVTVVAAGAAGSFVLWAGDGDLPGTSNVSFAAGRTRANNA
                     VIELARDGTRTIQVRNGAAAAAHLILDVNGYFR"
     gene            complement(13697..15466)
                     /locus_tag="PLP50_03015"
     CDS             complement(13697..15466)
                     /locus_tag="PLP50_03015"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPA50551.1"
                     /translation="MRWSNYFLSTLREAPGDAEVVSHKLLARAGMLLKAAAGIYSFTP
                     LGFRSLKKMTEIVREEMNRTGALEVELPIAQPKELWVESGRWDRYMNDGILFHLEDRK
                     ATEFALAPTAEEAVTDMVRRSVTSWRQLPFNLYQIRTKFRDEIRPRFGLMRGREFLMK
                     DAYSFDVDPKGLDAHYRKMDTAYRAIFERCGLEYALVQADSGAIGGSASEEFMVVADT
                     GEDALVFSEDGKYGANVEKAVTAPLPEPWANEEEKPFGRADSPTPGIVSTEGQAKHLG
                     TTEDRITNTMLYEAIRADDSSFVCAIVIRGDLTINEVKLPKALGVAHVRLVPEEWLPE
                     IAGCKPGFIGPIGLKPRAGQAEVPVYVDRSMENARNVFCGANQDGTHHSNLSVPRDLK
                     VAGVIEVATARDGDPSPTGTGVLRIKRGIEVGHIFKLGTKYSETMKCEVADPQQKMVP
                     LQMGCYGLGVGRTVAAAVEQNHDDDGIIWPVPLAPFQVALLSLQRDKEVLEAAEKIYA
                     ELTEAGVEVFWDDRDERPGVKFKDADLIGFPLRIVVGGKALAKGVVEVSTRREKKAEG
                     VPPARLLEVVKATLAAGAKTLAL"
     gene            15563..16837
                     /locus_tag="PLP50_03020"
     CDS             15563..16837
                     /locus_tag="PLP50_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50552.1"
                     /translation="MDAQPFDFVPVASPLHDAAALRRSLEPYLGALVGLGGRETGPEA
                     ATGSGPVAVVVGTGGTEAAVLRLREARQAVAPDEPLLLVALPAHNSLPAALEALARVR
                     QDGGRGRIVPLRGPDDGPGLARVAAALEDLRVREQLHRARIGLVGVPSDWLVASVPAA
                     SAVRETWGPEVVPAELGPLLDAPLRPADDATAGLAAAFTTGAATVRETDEAGVTTAAS
                     VLPALQALAARERLDAVAVRCFDLVTERGTSGCLALSALNDAGTVAACEGDLASAVAM
                     LWVKELLGTASWMANPSDADAVTGRLRLAHCTVPRSLLSGWDLRSHFESGLGVALAGA
                     LPPGPVTLLRLGGRRLESLFLAEGEALPTPRREDLCRTQLDVLLEPAAVAELLERPLG
                     NHLVLVPGHVAARLRAYREWAVGGEDGSPAAA"
     gene            16858..18603
                     /locus_tag="PLP50_03025"
     CDS             16858..18603
                     /locus_tag="PLP50_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50553.1"
                     /translation="MAVRYVSEEPHRVAPEHVGAELASPARRGVALAIDVPLLAALLA
                     LGCALAAAPSRAAAFCPAFPPATGDVVVVSTVAQLRTAVDAAVPGRTILVADGNYALN
                     GEYLRIDVPNVTLRGQSGNRDAVVLDGNYLTTEIVQVAASDVTVADLTLREAYDHPIH
                     VMPAGASATNRTLVYNVRIVDPGQQAIKINPTSDTGPYVDEGTIACSRIELTDAGRAH
                     VRDNCYTGGVDAHAALGWTVRDNVIEGFWCDAGLSEHAIHFWRNARDTRVERNVLRNN
                     ARGIGFGLAETGTPRRTYSDAPCPAAGGAYVDHFGGTAVNNFVSADDPDLFASEYGFD
                     CGICLWQACGASALHNTVHTADVASSFSAIEWRFSRTTATIANNLANFSLRARDGASA
                     TQATNVTTATAAFYVSPATGDLHLQPTAAGAIDQGSASSVGTDFDGDSRPRGAAPDVG
                     ADETGAPQALRFHPLAPCRVVDTRSASLGGPAPLAAGVSRPFQVSGACGVPAGVTVVS
                     ANVTVTQATASGSVVVHANGTPVPITPQVAFAAGRTRANNAIVGLDGAGLVSARAEMA
                     SGSVHLIVDVSGYFD"
     gene            18677..19339
                     /locus_tag="PLP50_03030"
     CDS             18677..19339
                     /locus_tag="PLP50_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50554.1"
                     /translation="MRLLPLTLLAALSLARPADAGLLDVATGAVAPGDAIAVAWTLPP
                     GFEESELLIQVDGGPRIRLTPECHDENPRFVIEVPALAGRARFVLRAGRVEEDGVHRE
                     HTIDSTEWFQIAPSFAAGPLPIRAPHTHPSEGEEMEWWSDSVGRVPEGPTSGLHRPWT
                     AEVSAATPSSPALSESRSEDLLSSESSVRGDVDARARNRNGLERGYRERSFPGAPVPL
                     RN"
     gene            19522..21708
                     /locus_tag="PLP50_03035"
     CDS             19522..21708
                     /locus_tag="PLP50_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA50555.1"
                     /translation="MLLTALPLLTLLLSPVLEAARYRVVDEKGRAVPGARVSILGRAG
                     SVAAGPQGELTLDAEPALPFEVGVFSPTGAWLGIVRIVTKSGADEVRDLVLPAARSTE
                     VLVSAGATASLLAPPANPVSLVSKRELEERQPNRLVEAIETLPGISKSDESTSAVPVI
                     RGLARGRTLVMLDDGRVSAERRAGASASFLNPFALESVEVVRGPGSVAYGSDALGGIV
                     HARTPLPEPGVFGVRYQVSGGVGGSPEASAGVGLNVPVGPAAFLVNLYQRNAGDYESP
                     VGEQDNSAVRDRGFLVRGAIPIGKARLFAGFQLDEVRDMGKPQADLPTTRTFYPWENS
                     RRFSLGLDVPDVAGFSSLELRALYGSYGISTNRQRRPNETTTLRDSISEVDADDFSVR
                     LLGRRSLGPVGLRVGVDVNGRTGLEARNVTNNYDVGGNPTSSTFEIAVEDASRTDFGA
                     FVEGDLPLAGDRLMLTAGLRGDQVKTENTGGYYGDRSTSDGALSGFATLTYTIAPEWK
                     LSAQYARGFRDPTLSDRYFRGVSGRGFVVGNPDLSAETSNQFDLSLRGRKGIFGFGVS
                     GYVYRIEDLVERYKSGNDNLFRNRGETEIAGAELEADVRITPRLVVRLLGSLSQGRIL
                     DDDTWAADVLPPTAQLIVDHEPFGGFFWRARLIAMARDERPGASEVVVAGHARLDLAA
                     GYRLSDLLTITISGRNVLDASYADSADENNVLAPGRGAILSLSGSF"
     gene            21840..23087
                     /locus_tag="PLP50_03040"
     CDS             21840..23087
                     /locus_tag="PLP50_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA50556.1"
                     /translation="MPLLRRPRALYAAFDRFPTRKGASTHIARFAPALFEAFGGGLLY
                     VLGDERLPAHQVEGEVEVVRFSVPVENFLERAVAFGAHLSRLLDESGEALEVCHFRDP
                     WSGVPILERERRYATLFEVNALPSVELPYAFPSIAPRTLEKVLAAERFCLDAADRVVT
                     PSRTTRDFLVAQGVPAAKVAVVPNGADPIPPRPRPFEAPDAYLIYFGALQPWQGVDTL
                     LRAFARLADLTELRLVVCGSARSRPARAAEKLAEKLGLGERVTWRWELPTAELEPWLA
                     NALLSVAPLRATERNVVQGCAPLKVLESLAAGVPVVASDLPPVREIVTDGVEGRLVPP
                     DRPADLARAIRLLLHLPEERERMAAAARARAARDFGWDRSVALLKDVYRALRPTAATP
                     SAPRPIEEEPDVRSAEGPKVAVA"
     gene            23047..23922
                     /locus_tag="PLP50_03045"
     CDS             23047..23922
                     /locus_tag="PLP50_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50557.1"
                     /translation="MFEALKARKSLSPEQVTLLQTKQLAGEYEPGALVALLKPIAEYD
                     RLSDKARTPMGCTTGALFVLAFVLLIVSANVSFYLLPLPLAALGAGIALLVTVLKMKK
                     LDLSNNFRQVAMPLFAVLREDMEPGATMNVRLDLSPPTAKGKKIGTGKPYDVPGYRKV
                     VDTTYVDPWFGGGARLADGSVLQWTVTDDVVASERTKRTARGKIKTKTKHRKLTTIAV
                     DVALPKKEYAVAAMPADEGEKVKVKDGEKRTSLHVEKRVKTKSLDPIDPRAVFDAIAE
                     AYRRVRPAGAEGSAS"
     gene            23919..24299
                     /locus_tag="PLP50_03050"
     CDS             23919..24299
                     /locus_tag="PLP50_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50558.1"
                     /translation="MSGCECKGGFFTLRDCDGAPAGACSECGRTACARHLSPASGFTQ
                     CLDCHARAEQQPREGQNATGEEVLDDEWTYGARHRFYTAGYAPLYAGSHRSHYYDSYD
                     TRSFDDEGVTEAETDEGGAGFGDS"
     gene            24301..25464
                     /locus_tag="PLP50_03055"
     CDS             24301..25464
                     /locus_tag="PLP50_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA50559.1"
                     /translation="MRPLVLTENYPPDRGGMAQSCDRIVRALRASGVDVDVAHLGRRA
                     VRPFVERQERGLLLAAPADEDPAHATQLLWNEVARLPHRPTHVVAFGGASPVLAAPVF
                     AAWLGVPLVTLLRGNDFDTGLFSLRRGWALRDALGRSAAVATVSRDHQRKVSALFPAA
                     DVRYVPNGIDPAEWVLAPHDLERAARWRAEHVGEGRRVLGMFGHLKRKKGGAFLLDAL
                     RRAGVEGRLHLLVVGEAEPEMLAHLASIESSVPFTHVPFVDRFELLPYYAACDGVVVP
                     SLYDGLPNVLLEAGALGVPAVASTAGGMADLLEGGGSGLTFPPGDLHECGRALATFAA
                     LPATELRTMGERLRALVLRDFRHDQEAARYRALLAETAGRTSRPVPADVLTES"
     gene            25468..26073
                     /locus_tag="PLP50_03060"
     CDS             25468..26073
                     /locus_tag="PLP50_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50560.1"
                     /translation="MKNPLARLLPFALLAVPLAAGAVTPADVHTVVVRSAGGSKSFTI
                     VENGNAIEIVLPGGVRLLGEPAGEKRKYRRGAGGPPVCEVKPGDAGFKLRTTDGKLLW
                     KVKLADDKIKVSDNEENANPYSIKLKYPDKAKVVDAAEKELAEVRFGPDKTKVKGADG
                     AELWECRSPRRRTASFGVLVLERIPEEHRAILMAEILARRR"
     gene            26081..26932
                     /locus_tag="PLP50_03065"
     CDS             26081..26932
                     /locus_tag="PLP50_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50561.1"
                     /translation="MLVYYALGGGLGHLVRARAFLHTLGLETEATLFTSSRFADDHRV
                     TGGLPVVRVPDSLNGDRAGLRALLEEKIADTGATRLVVDAFPAGLLGELAEWAPPAGL
                     ALWHVARLLRWDRYTADASPRLPRFDVTFVVEELHVEHRRALEAATRELRPLDLVDPP
                     APPSEPIEGPHVLVVHSGPDEETLDLVACARELAAADGRGARVVLASPARPGSLPADV
                     EWRDAFPATGLFPAADRIVAAAGFNAVRQTEPFRAKRFLVPYPRRFDDPFARAARVRC
                     PQARCQA"
     gene            27108..27698
                     /locus_tag="PLP50_03070"
     CDS             27108..27698
                     /locus_tag="PLP50_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50562.1"
                     /translation="MRDAAVRCAHAPRTTFLVLLVAYATSLHGAPPADRPAGGATAGA
                     VPAAPAVSGFARVDDTELRLAHGYLLRAPDAFKASQQNAIVLLTPQPLDPKKLDAAGT
                     LLAALDLSPQRVVLEVEPDRSVRLWICHDGFGAGKCFITPIAPFDWKPGVVEEKHVSG
                     SLKSFAGKEETVLETFRLYYEVQFDVKGGRALATRR"
     gene            27837..29333
                     /locus_tag="PLP50_03075"
                     /pseudo
     CDS             27837..29333
                     /locus_tag="PLP50_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            29405..30649
                     /locus_tag="PLP50_03080"
     CDS             29405..30649
                     /locus_tag="PLP50_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50563.1"
                     /translation="MSAALTPPPAWGPGPLPPPAPGGTVSVRPLPNGEEIVLRATGWL
                     RLLPAAFLLFWLCGWAVGELLVLLILLGPLADPLVSALREVLPGAWPRPPRFAGSTPW
                     PAVAFLAVWFVFWTWGGLTAAWQLLRLVAGSDRFVTRPGGFSLRPGAGPFGRTRDFPA
                     SEVSAVYADVRRSRLVAVVRGSERLLCSGTPPPVAEWLAARLRAALGLPGPTLADSGE
                     RDAEASAEPPVPVDWEARPLPEGGVLLSAPPARSRKTAGCALAVAVVVTAGFVAVLLA
                     AGVRRESPGAALVFGLLILAVDVLCSWLAFARESWLLRPGLIARVRSLGPWERRREVR
                     HGSLAVSVSTDSDDDDWFRLEARGEGRPLKLSSRINAGPEVLAFARYAARHSGFPLDV
                     PHEVLLAAGAPRNGSSRARGPA"
     gene            complement(30675..31883)
                     /locus_tag="PLP50_03085"
     CDS             complement(30675..31883)
                     /locus_tag="PLP50_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50564.1"
                     /translation="MSAAVACVLAASALGVTGTTGRSAPEAYVRALEERGRTPVDLVL
                     GALEKHDLLIFDDALHNAVEPFELYASLLRDRRVRARVRTVFLEVFSIAAQPHLDAYL
                     ASPTDDRSLLAPAFRDDFSGYGWRYSTYLELLATVRQLNQGLPPSERVRLVGVDQPIW
                     WDAIRTRADYELFQDSLAGRDFFLYATIRSRLDGFREGEKAFFLTNTRHAYMRLRDGR
                     GRPQLNAAGFFGRDDPGRAWSARLHNATLAVEGVVHAAGPRSTQGLESKSYRWVRMED
                     GIWDRAFAANGNVPVGLELAGTPFGAARFVGNTMLDAAPGSTMADAYDALIFLGPIDE
                     LRLSGTTGFYVTPEFRPELERRIRLVEGDGLARALAARGVSTVDALLDGLAAGTPARR
                     NGLLDPPAKP"
     gene            complement(32158..32661)
                     /locus_tag="PLP50_03090"
     CDS             complement(32158..32661)
                     /locus_tag="PLP50_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50565.1"
                     /translation="MKRRHARANGLASLLCAAVVLSTGCFSFPAMPLPGYAATETAPA
                     GALGSFAVSSPLLGNTTVAPTGCVSGDLQQFLGGDFSDPASGLVVRLVVDPLEGPAAR
                     VFDAADPFRQSVVLRRSECAVFHFSLEHTGLTINEVRDYRLSLELDCALADGTTVHGR
                     ASTTHCH"
     gene            complement(33044..34465)
                     /locus_tag="PLP50_03095"
     CDS             complement(33044..34465)
                     /locus_tag="PLP50_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA50566.1"
                     /translation="MGEAERRTPVGWALASLALPMLLSSLGTSIANVALPALATSFRA
                     PFHAVQWVVLAYLVATTTLVVGAGRLGDVTGRRRLLSAGILVFTLASALCGLAPTLGM
                     LVAARALQGVGAATMLALTLAFVGETVPRERTGAAMGLLGTMSAVGTALGPSLGGLLI
                     AGLGWRAIFLVNLPLGAAALVMARRYLPADRRAAKAGRPRFDLAGTVVLALTLASYAL
                     AMTVGRGGLGARGAALLTGAALGVALFVRVEARAKAPLVRVAAFQDPALGAGFAMSAL
                     VSTVMMATMVVGPFYLARALGLDAGSVGLVLSAGPLVAAMTGVPAGRAVDRFGAPRTT
                     LAGLLGIVAGAVGLSMTPAAAGVVGYVIPIVVITAHYALFQTANNTAVLADVDAGERG
                     VVSGLLNLSRNLGLITGASVMGSVFARASGAADVAAADPAALAAGMRATFVVAAVLVV
                     VALAVSRASRAPAPVPSPAGEMS"
     gene            34644..35621
                     /locus_tag="PLP50_03100"
     CDS             34644..35621
                     /locus_tag="PLP50_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPA50567.1"
                     /translation="MAAPDLNLLVTLAVLLAEGSVARASRRLGLSASAMSRALARLRA
                     TTGDPLLVRAGRGLVPTPRAIALRDRVGPLVQEAEAMLRPAENIRLDTLERSFTLRSA
                     EGFVESFGASLLARVAREAPGVRLRFVQKLDRDNVPLRDANVDLETGVVDDTTDPEVR
                     VQALFRDRFVGVVRTGHPLCRGKITPARYAAGRHVFVSRRGRSTGPMDDALKRLRLER
                     EIDTVVGGFSTALALARATDLIATVPERHTGSLRDGMHSFPLPVATPGFTVSMLWHPR
                     FHADAAHRWFRGCVKEVCEEPPGRATLAARPPARSVRRVGSRRGTVHSL"
     gene            35940..37250
                     /locus_tag="PLP50_03105"
     CDS             35940..37250
                     /locus_tag="PLP50_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50568.1"
                     /translation="MIAFASLWVGFVIGIVNVQLIASEGVSRVELILDGRPVAELRAP
                     FAAPLDLGCDPSPHELVAVAFDAKGKELDRARQWLNRPRPTAEASLVLEGGHGGSGRV
                     ARLTWRALAREAPTSVAATFDGTPIPVSDPARIELPPHEPAQIHFLKTVLEFGTGVSA
                     TAELIFGGSKTVEEYTELTAMAVELERKASLPPVETMAGWFEENGVPLEVAAVDAGGV
                     DIVFVAEGSARKELKQAFRRESLNDAETAANLRNGARFLREDLRYRFLWPVGRLTAQT
                     EMVANVYQSTPWLSRKRSSIGRAAGWLLDWPGWSRDGQRLADAVAVAGLTAAEANRRR
                     AVVLLLGPAAKDGSLLTPDEAMSFLRRLGVPLAVWSIGKRSPETSRWNARTLISAYEH
                     REFDDAMRALLESVDRQRIVWLEGAHMPHEVETSRFAHGIRRVQ"
     gene            37324..38601
                     /locus_tag="PLP50_03110"
     CDS             37324..38601
                     /locus_tag="PLP50_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50569.1"
                     /translation="MISFATLCFGVVYGVLNVEIRASDDVARVDLVLDGRPVAELRAP
                     FAAPVDLGWQPAPHQLVAIGYDERGKELGRAKQWINRPRGNAEVHLLVEPEGTERGRL
                     ARLTWSCPTSGAAGPVEVTLDGRKLPVSDPSRIEIPVRDASRAQILRARVECGGNVTA
                     MTETVIGGDRPSTGSPQVTAVPVHLEKGTKLPSPEGLTGWLERDGVGLRVAAVEEGGF
                     DVVFVCEGGAFEELTRLFERRGAPVRDSSEDDLRFRFLVPVGRTVDDPTVDSSSYAAT
                     AWFQRRWDAIGPTLTNGLQLKPWPRTEQRIADAVAAAGLAAAARERRRAVVLLLGPGA
                     RDGGDLNSGEARQFLMDLRVPIFVWSIGKAPSPETGTWDATSTITTAAQFDVAVTRLR
                     AELRRQRILWVEGAHLPQVITLTPRAKGVRLAR"
     gene            complement(38882..40507)
                     /gene="pruA"
                     /locus_tag="PLP50_03115"
     CDS             complement(38882..40507)
                     /gene="pruA"
                     /locus_tag="PLP50_03115"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPA50570.1"
                     /translation="MSLGIYRVPVPKNEPVKDYAPGSPERASLQKKLAEMASERIEIP
                     LVIGGKEIRTGKLAKAVMPHDHGHVLADVHLAGPEEVALAVAAADRAKDFWSRMPWEE
                     RVAIFLKAADLLAGPYRDVLNAATVLGQSKTCHQAEIDSAAETIDFFRFNAHYYAEIL
                     TEQPESGPGMWNRMEHRPLEGFVFSVSPFNFTAIASNLAAAPALCGNTVLWKPATTAL
                     YSAYWLTRLYRDAGMPEGVINMIPGSGRAIGDVVLNHPDLAGIHFTGSTGVFNGMWRT
                     VGQNLEKYRSYPRLVGETGGKDFIFAHPSADPKALTTAILRGAFEYQGQKCSAASRAY
                     VPSNLWAKIQDELVAEVEGLKVGDPRDFTNFMGAVIDEAAFKDHREAIEAAKASKDAK
                     IVAGGTYDSSVGWFVRPTIIQALDPSYHTMCHELFGPILTIHVYDEAKWEEMLDVVDA
                     TSPYALTGAVFSQDRYALHVMVHHLRHAAGNFYINDKPTGAVVGQQPFGGARMSGTND
                     KAGSSLNLLRWISPRTIKETFVPATEWRYPYLG"
     gene            complement(40542..42077)
                     /locus_tag="PLP50_03120"
     CDS             complement(40542..42077)
                     /locus_tag="PLP50_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPA50571.1"
                     /translation="MSLKTETDVEILRALGIEERNPGGFSGAWVGSGKVQQVVSPVDG
                     SPLASVVNVTKEEFDGILAKTHAAFASWRTVPAPKRGEVVKAIGEELRAQKEPLARLV
                     SLEMGKTLRESLGEVQEMIDICDFAVGLSRQLYGLTMPSERRQHRLQEQWHPLGVVGV
                     ITAFNFPVAVWSWNAALALVAGDTVLWKPSSKTPLTAIAVTKIARRALERFGYDPAIC
                     SLTIGRGSDIGDLINTDPRVALVSYTGSVPGGRHVGKLVQERFGRLILELGGNNAVIV
                     SEKADLDIAIRAVYFGAIGTSGQRCTSTRRVILHESVYERFVERLSALYAETTIGDPL
                     AYDTLMGPLVDRDAVATMRKAIETAKGQGATVLRGGEPLEKEGGCYVTPCLVEASSDL
                     PIVKEETFAPILYLMKYGTIEEAIAMQNSVPQGLSSAIITNDYRETELFLSAEGSDCG
                     IANVNTGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNALNFSGRLELAQGIT
                     LEI"
     gene            42165..43067
                     /locus_tag="PLP50_03125"
     CDS             42165..43067
                     /locus_tag="PLP50_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPA50572.1"
                     /translation="MSTLDLSQLRAFHAVARAGGFSAAAARLNLTQSAVSHAVGKLER
                     SAKVRLFDRRGRKVVPTPEGAILREACERVFATLEAAEEELQRERSRAEGRIRLGATV
                     EFGTSVLMRHIRPFLEANPGIEMDFHLSHDLLTPLLRDDVDLAIDCVAHATPELERTP
                     LFRETYMVACAPDYLAAQRIAEPADLARCAVLSIDKEGRWWERFLGAFPKSRRPSFGR
                     VVAINHIRGLITAAASGLGVLLAPSYSVKGELDRGALVPLFPAFRPLEDRFALYQKAG
                     KARLAKHRLLTEYLERLTPEEFGA"
     gene            43204..43860
                     /locus_tag="PLP50_03130"
     CDS             43204..43860
                     /locus_tag="PLP50_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPA50573.1"
                     /translation="MARLKVASFSISLDGFGAGPDQCLENPLGFGGAQLHAWAFPTRT
                     LRRKVFGHDDGETGLDDDFVARGFEDVGAWILGRNMFGPVRGPWPDESWKGWWGEEPP
                     YHVPVFVLTHHARPSLEMEGGTTFHFVTGGIHEALERAREAAGERDVRLGGGPATIRQ
                     YLAEGLVDELHLAIAPVLLGSGEPLLAGLDLRALGYACTRHVASERATHVVLERTRHT
                     "
     gene            43893..44330
                     /locus_tag="PLP50_03135"
     CDS             43893..44330
                     /locus_tag="PLP50_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPA50574.1"
                     /translation="MPNTIRLHRVLKAPPDRVYRAFLDPDAMVKWLPPNGFTGKVHSM
                     DARVGGSHRMSFTSFTTGSSHSFGGTYLELVPGERLRYTDAFDDPNLPGGMEVTVTLK
                     KVSCGTELQITQEGIPDVIPPEACYLGWQESLVLLGKLVEVAE"
     gene            44378..45289
                     /locus_tag="PLP50_03140"
     CDS             44378..45289
                     /locus_tag="PLP50_03140"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HPA50575.1"
                     /translation="MDYPRILAEIAEEVRPLVGQGRAAQYIPPLAEVPRDRFGMVLRT
                     VGNEAYSVGHARERFSIQSIAKVFSLTLATRLEGDAVWERVSREPSGTAFNSLVQLEV
                     ERGIPRNPFINAGALVVLDMVLSHQREGEDLVLDFVRRAGASPDVGYDPVVAAAERAT
                     GHRNRALAHFLKSFGNLRNDPDRVLDAYFLLCSISMSCAELASAFQYLTTGGLTAGGE
                     EVATARQVKRVNAVMLTCGVYDEAGDYAYRVGLPAKSGVGGGVVAVMPGRFVTAVWSP
                     CLSEKGNSLAGTMALELLTTKTSLSVF"
     gene            complement(45708..46622)
                     /locus_tag="PLP50_03145"
     CDS             complement(45708..46622)
                     /locus_tag="PLP50_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HPA50576.1"
                     /translation="MNTTTTYDYREILAASEKVRWTVDDLMGGHALDFSRPFLPESLA
                     RVDPLDFLTPAEKRTLNQVRGNSYLCIFGLVEEFILPFVLDHVRTQLSGEDERVRAML
                     QFAGEEAKHIHLFKRFREEFTKGFGTECAVIGPPEAIAKAVLAHEPLAVALAILQIEW
                     MTQRHYLDAVRDDAGIDPMFRDLLKHHWMEEAQHAKLDALMVEALAEGKDAEALSRAV
                     DGYLAIGGFFDAGLEQQVRFDLEAFERATGRRLTEGEREKAMAVQRQASRWTFLGSGM
                     THPEFLRSLGRMGPAVREKVEKVAPAFC"
     gene            complement(46664..47257)
                     /locus_tag="PLP50_03150"
     CDS             complement(46664..47257)
                     /locus_tag="PLP50_03150"
                     /inference="COORDINATES: protein motif:HMM:NF023363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HPA50577.1"
                     /translation="MTRSKKAGSARGLLIATVLTLGVAPSALAGHVEPPPVPAGLEVP
                     RGNRPFLLAHAVGTQNYVCLQAGAAWTFLGPQATVFDDESAQVMTHFLSASPAEGGIH
                     RATWLHSRDSSAAWAMAIASSSDPDFVAPGAVPWLLLRVTGTGDGPTGGDRLAPASYI
                     QRVNTEGGTAPAGACATAGAKLFVPYTADYVLYRERR"
     gene            complement(47438..47671)
                     /locus_tag="PLP50_03155"
                     /pseudo
     CDS             complement(47438..47671)
                     /locus_tag="PLP50_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(47795..48376)
                     /locus_tag="PLP50_03160"
     CDS             complement(47795..48376)
                     /locus_tag="PLP50_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HPA50578.1"
                     /translation="MSPLHMLRFVVQQGLPEPAPHARELQYDALAFQPRALVVLVAGA
                     ALSREPVVWAAIAGLLLLGAAAPRLNPFDALYATLHRRQSRRLFLPGARAPRRFAQVL
                     PALFCLTAALALLDGREAVVWGVVVAFGLAFAGIFVFGFCFGAWLFRLLVPSWRPPAE
                     RAARSASASAAPVAGLAEGAGLRTPPPDPPRDR"
     gene            complement(48457..51405)
                     /locus_tag="PLP50_03165"
     CDS             complement(48457..51405)
                     /locus_tag="PLP50_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA50579.1"
                     /translation="MRELPDRDEWAVLDGLLDAALDRPPAERAAFLEAACAGNEETRR
                     RLERLLAWAEEEDGRLAPSGAVAGPLFAQVARSLADAPAEDVLAPGDSIGPYRVVGSL
                     GSGGMGHVYRALDPQLGREVAIKALARAFHDDAASLRRFEREARALAALNHPGIATLF
                     ELLEVEGRPYLVLELVEGETLAERLERGPMGAVEAVEAARQIVDALAEAHGKGIVHRD
                     LKPSNVKLGEDGRVKVLDFGLAKTAPVSGGNGAGTATTLSGTILGTAPYMSPEQARGL
                     QTDRRTDIWAFGCLLYEMLSGTRAFEGRTVSDILASVLRDEPDFGRLPPATPAALVRL
                     ARRCLKKDPKERLQDVADARADLEEAVAAPGRRDGRARPPARPAVRRALAASAVVAIV
                     LAAGAVARRSGPARVEPVRLGLDPGEPLDESFAAPFALSPDGRTVVLVAREGTSTRLL
                     LRRLDQFDSHPLAGTEGARQPFFSPDGSSVAYFADRKLWRLPLAGGSPVALAEVGNNP
                     RGGSWGADGAIVVGLSQTSGLHRVRADGGPLEPLTKLDLATGESSHRWPQVLPGGRHV
                     LFSVLTEGSSWDDAVLEVLSLEDGARRVVQREAAFGLWAGRGPLLYAHAGRLYAASFD
                     PVRLVTTSAPEVVLEGVAYSPRNGGTKVALSASGTLLYAAGRPTSLENHLAWVDTAGR
                     ATRLPGVPRRFREPKLSPNGGRLAVRIGGADDAELWTLDLATGTLARVTTGLSPRRPI
                     WSPDGTRLVAGARSGARWALFAMPAAGGDGAVLYESANRVYPTSFAPDGRVLVFQEQR
                     PGQGWDLRALALGDGLRPEGEPRDLAAGPANETNGVVSPDGRLLAFETDELDAVVQVY
                     VSRLPADGSGKVQLTREGGRFPRWWSAGEVLYLQTPEWEPRRVPLREKGGRILADEEV
                     PFWTEEPAAARARASLFIPNYLGFDLDAGRRRLLVLEQPAPSETPSPSGLRVVLGWLD
                     ELPERGLRKD"
     gene            complement(51402..51983)
                     /locus_tag="PLP50_03170"
     CDS             complement(51402..51983)
                     /locus_tag="PLP50_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPA50580.1"
                     /translation="MAEPISVTALLRAHAAGEEGAFDRLLPLVYEELRKVARRQLRRG
                     GGAGPLVTTELVHEAYLRLAGQDGLRLEDRRHLLAVSALAMRQIVVGQARARYALKRG
                     AGVPPVPLDEEATAAAADAEWLLDLDRALERLRDHDEKLAAIVDCRYFAGMSEEETSE
                     ALGLSLRSTQRGWMKARAWLRSALEGGTPEKAP"
     gene            complement(52045..52878)
                     /locus_tag="PLP50_03175"
     CDS             complement(52045..52878)
                     /locus_tag="PLP50_03175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPA50581.1"
                     /translation="MTPPERLSFRHRVVAGDPPGAADVLARVSGLSKGVVKDAMRKGA
                     VHVGRAGRTPNRLRRETATVRPGDVLEIHYDAALLALVPPEARLVRDAVRWSAWDKPA
                     GLLTQGTPFGDHATLLRQAEKALGRPALPVHRLDREVSGLVLVAHDEQAAAALSALLR
                     EGRVVKRYRAEVLGDVASALGREGVVDTPLDGKSARTLWRVVAHDAERGATSLDVTIE
                     TGRQHQIRRHLESIGHPVLGDPRYGAGNKNRDGMRLAAVFLAFRDPYTGEEVALSTHG
                     K"
     gene            52975..53349
                     /locus_tag="PLP50_03180"
     CDS             52975..53349
                     /locus_tag="PLP50_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50582.1"
                     /translation="MRATCRLVLLLFATLATAGCGCVWEGRNVWLATEASAGKTLEAN
                     ARDEVLLELPVEAGSKARWYVVEVDEKVLRRKGPAEEHSGGRVRIPFEALRTGVTDVV
                     AFYATARSAPPERTFRTTVWVR"
     gene            53402..55330
                     /locus_tag="PLP50_03185"
     CDS             53402..55330
                     /locus_tag="PLP50_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPA50583.1"
                     /translation="MNGFPLLPDAEWLETDGLGGYALSTVSGRSARRYHGLLVSATTP
                     PTGRAVLLAKLEETLVVGDERFDLSINLYGGKLHPRGDLLLRSFRVDPFPTWEYEAGG
                     ARLRRTFFLVHGEPTAVVTWELLSGGAPVRLEVRPLLAYRDHHALRGVETPCDPALVR
                     EEGAVNLSPVCALPALRLAHDAASATETFFWYRGFEYDLDRARGFEFTEDLFSPLAFA
                     WDLADRPAATLVATLAGRSAADAPGLAEAEERRRRKVEGAGSDRRALRVLRNAADRFV
                     ARRGEGWTIVAGYPWFTDWGRDAMIALPGLLLATRRFDAARSVLETFASHVDRGMVPN
                     RFPDEGTEPEYNNVDGTLWFVEAVRAYHEATRDDAFRRQVYPLLVEIVERHLRGTRFG
                     IRCDVDGLLSSGEPGQQLTWMDARVDGRVVTPRAGKPVEVQALWHNALRVVTLYAADL
                     GDAFTAARLLAIADQARESFSALFWDEGVGWLADVVGPDGTRDLSLRPNQLFALSLHR
                     PLVTGRRAERALSAIEEKLLTPYGLRTLDPAHPDYRGRFEGGPAERDAAYHQGSAWPW
                     LLGALVDAHLAVRGNGPASRRRASAWLAPLLEHLLGPGLGQLPEVFDGDPPHRAGGCV
                     AQAWSVGEVLRAALKTGG"
     gene            55576..57183
                     /locus_tag="PLP50_03190"
     CDS             55576..57183
                     /locus_tag="PLP50_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HPA50584.1"
                     /translation="MKAHAATAARPSPLALPLEGSPAEEAALRGGLHLEEQTAERRRE
                     LGQFFTPVPVARFMARLARPGRRVRRILDPGAGSGVLACALLEELPDGAGPVHVDAYE
                     VDHGLARVCHDSLSRALAWLLGRGIEMTFKVHQTDFVRANAACLSTSLFESATPDPYD
                     VAILNPPYFKLAKADPRARAAAEIVHGQPNIYALFLAITASLLAEKGVMVSITPRSFT
                     TGDYFRSFREHLFSQVTPETVHLFDSRQDAFSKDEVLQENVIVRARKAKPAPDASVQV
                     STSAGVSDLGRPRTRTVPLESVVDLRSRDFVLHIPTAETDDTVLSFVRAWPQTLGSLG
                     LSVSTGPVVAFRARESLLYEADAAEEAVPLLWLQHVQSMAVRWPIPGVHKPQFFRVEP
                     STQKLLLPSDNYVVMRRFSAKEEARRLVAAPLYGRELSESTLALENHLNYIYRLRGSL
                     TREEATGLSAVLGSALLDRYFRVSNGNTQVNATELRALPLPALREITAIGEELLESPD
                     AAARAEEVMTRILHVPATLRAEIGRHG"
     gene            57176..58117
                     /locus_tag="PLP50_03195"
     CDS             57176..58117
                     /locus_tag="PLP50_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HPA50585.1"
                     /translation="MGKLEDGKKILKALGLPPQQQTDIASYTLLALADVGKRVPWASA
                     DRRSMKIHAIKEWVGETYGKVYAENTRETFRRQVLHQLEQARVVDRNPDDPTLPVNSP
                     RTHYALTADALAVVRAFGTSAFKAEAEKFGKSHGALLEMYRAARVGQRVAVVLASGKK
                     LTLSPGEHNELQKAIVESFAPVFAPGARLLYLGDTAAKDLHVETGALEALKIPVTKHG
                     KLPDVVLHMPEKGWLLLIEAVTSHGPVSPKRKKELELALKDCSLPRVYVSAFPDRDEY
                     RRHMADIAWETEVWIADSPTHMIHYNGEKFLGPHAPY"
     gene            58198..59526
                     /locus_tag="PLP50_03200"
     CDS             58198..59526
                     /locus_tag="PLP50_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HPA50586.1"
                     /translation="MSLRYESFPKTILEIIHSFEKGRLNLSPGFQRDSVWTDSDRQKL
                     IESILRGYPLPSVFFYKRETDGQIYFDVIDGKQRIETILRFTGRIRGNRFATKKIQLP
                     GEDEKDWVDWDKLKRRRQQHKIESYTLLTIQVDGELSEVIDLFVKINSTGKALSAAEK
                     RKAKFYDDDFLKEAARLARRFEGYFKAQHILSVGQILRMKNVELMCELMLSISKGDVI
                     NKKAALDRVMQSGSMTAHQLEKASRLTVSALNRVRRLFPDLGRTRFSKISDFYSLAVL
                     VARLESEGLILTDRRRNRLAWDLLVSFSNGVDTVRERQRRAEGAGPGEELYREYLLTV
                     LEGTDEITKRQKREEILRGLLGSLFERTDERRLFSSEQRRILFNSATDRKCTAKGCGR
                     RLGWDDFTADHVRPFSQGGRTVLSNAALLCRKHNSAKGNRRPHRGGRRSR"
     gene            59545..59664
                     /locus_tag="PLP50_03205"
                     /pseudo
     CDS             59545..59664
                     /locus_tag="PLP50_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
     gene            complement(59674..61116)
                     /gene="gnd"
                     /locus_tag="PLP50_03210"
     CDS             complement(59674..61116)
                     /gene="gnd"
                     /locus_tag="PLP50_03210"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPA50587.1"
                     /translation="MADGRADIGLIGLAVMGENLVLNFESRGFRVAVHNRTVEKVDAF
                     LEGRAKGRNVVGARSVSELVAALSVPRRVMLMVKAGAAVDGLIDALVPHLSPGDVLID
                     GGNSRFEDSARRAGELEARGLLYVGAGVSGGEEGALLGPSIMPGGSAAAWPLVAPLFR
                     AIAARAPDGTPCCDWVGSDGAGHFVKMVHNGIEYGDLQLLGEAYHLMSRLLGMDADEM
                     SGVFDGWSRGDLDSYLVEVTRDVLAARDADGRPLVDAILDVAGQKGTGRWTSATALEL
                     GVPLGLIDTAVSARFLSTLKEERVAASTVLPGPSPSFAGDRAAFVDDLGKALFAAKLV
                     SYAQGFALMRAASEGRGWGLRYGGIALLWRAGCIIRSSFLDRVKEAYEAEPALPNLLL
                     DPWFRGKVGEAQDAWRRVVSAAALSGVWAPGLSAALAWYDGYRSARLPANLLQAQRDY
                     FGAHRYERVDRPRGELFHTEWGAGSGSGRD"
     gene            61339..62445
                     /gene="phoU"
                     /locus_tag="PLP50_03215"
     CDS             61339..62445
                     /gene="phoU"
                     /locus_tag="PLP50_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPA50588.1"
                     /translation="MPTRLEESLGRDIDRIRQQLLEMSALAERNLRDCVEAMKGGERA
                     RAYAVILRDQLIDEKEKEIDRLCLEFLVRQQPVGFNLRLAYSAIKINLELERVGDYAE
                     SIARQVLRLEKLPATPETHTGRLVEMADLAIPMIHDATRAFLAQDADLARAVVAREPA
                     VDALLGTLVTELLDEVSAGRIGAEMLVALSTIGRRFERVADQARNVCMETLYMCTGQY
                     AKHPGAETFRVLFVGEHNACRSQMAEAIGQSLDQSRFLFSSAGLSPRAIDPRTAEFLT
                     EKGYDAARMAPKGTDQVPNLDYYNVIVGVAPEAQSLFPRTPRNLVYLDWSTADPSRAA
                     GSPEEVRAAYQATYEFLERNIRDLVGAVLDGTHD"
     gene            62470..63459
                     /locus_tag="PLP50_03220"
     CDS             62470..63459
                     /locus_tag="PLP50_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPA50589.1"
                     /translation="MPRAAFARPLLLLAAVAALGLPACGRRDGGASPSNVIQNKGSDT
                     MVNVAQVWAEAYRKAAPDVEVEVSGGGSGVGIAALVKGAVDIANASRDIKPSESEMAL
                     ANSGKAPVDFKVGYDALAVFVHRDNPVSELTLAQLKEIYAEGGRALRWSDLGVTIPGV
                     SDDRIVRVSRQSSSGTYEFFRSRALDDEDFRLGSLDLNGSKEVVELVAVTPTAIGYSG
                     MGYATDHVKMLRVSPGTGVPGVGPTVDAVHDKTYPLARSLHLYTLGPPEGAVKDYIDW
                     ILSPAGQEVVRVAGYVPAVVAPAPEAATAPPAAPSSPAPAPSAPPAPEAAPAR"
     gene            63456..64379
                     /gene="pstC"
                     /locus_tag="PLP50_03225"
     CDS             63456..64379
                     /gene="pstC"
                     /locus_tag="PLP50_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPA50590.1"
                     /translation="MSASTTAAPRPERPRLSVLGEKALETLIRLAGVSAMLFVAAIFF
                     FVLREAFPVLANEKFSLTQFLFSTEWYPTSAKNVRYGTFALTIGTLSVTALAILIAVP
                     FGLGAAIYLSEFCAPRLRETLKVVIELLSAIPSVVWGFIGLTVMSRLVVSVTGAPVGV
                     NLLNGGILLALMSVPVIVSIGEDALKAVPDSYREAGVALGATRWQLVWRVLLPAAKNG
                     LLAAVLLGVGRAVGETMAVLMATGHAVNIPGGILEPVRTLTANIAAELGEAPSGSPHY
                     RVLFLTGTLLFLITFVVNLAADFAVRGVRRK"
     gene            64382..65275
                     /gene="pstA"
                     /locus_tag="PLP50_03230"
     CDS             64382..65275
                     /gene="pstA"
                     /locus_tag="PLP50_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPA50591.1"
                     /translation="MAGRFAKTPHGLRKERTEALAKAVFGLMASAMILPLLLIVGYLV
                     VLAWPALGWEFLVDVPKNGMRDGGIWPAFVGTLYLVGISLVVSTPIGVLAAIYLNEYA
                     KDSWFNRLVNLAVINLAGVPSIVHALFGLGAFVYAARFGYSILSASLTLAIMTLPVII
                     ASTREALASVPTAFREACWNVGATRWQTIRTVVLPNSISGILTGVILQVSRAAGETAP
                     IMFTGAVFFKAVEKGDLFPYGLGEQCMALSMHLYTLATQVPNVKEALPYATAVVLLAT
                     VLLVNATSIAVRIWLRGRKKW"
     gene            65269..>65956
                     /locus_tag="PLP50_03235"
     CDS             65269..>65956
                     /locus_tag="PLP50_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA50592.1"
                     /translation="MVAGSAAPLEIEVHDLRLSYSGREVIHGISFDLRKNEILAVIGP
                     AQSGKTSMLRCLNRTIDFIPTASVSGTVKVHGEDVRRTKDVFALRRRIGMVAPLPVGL
                     PLSIYDNVAFAPRCAGVTNRADLDPVVEKCLRQAALWDEVKDRLGSLGTALSGGQQQR
                     LTIARALSHRPEILCLDEFSIAIDPVTTMRIEDVLKELKEEITIVLVTNLTQQARRLS
                     DRTMFLLEGEI"
BASE COUNT         9205 a        24419 c        23185 g         9147 t
ORIGIN      
        1 tcgagacgaa gctcccgatc ccgtacgcct cccgcgccgt cttctcgccc gacggcaaga
       61 cgatcgccta cacgccgaac cgtcccgcgc acctccagtg gaagcgctac cgcggcggca
      121 ccgtctcgcg catctggctc tacgacgtcg cgacgcacgc gatcgtgaag gtcccgcagc
      181 ccgaggggcg ctgcaacgac ctgaaccccg cctgggtcgg cgacacgctc tggttcacgt
      241 ccgaccgcga cggcgagttc aacctccaca cgttcgacgc gaaggcgagg tccgtcgcga
      301 aggtgacggc acacgaggac ttcccggtcc tcgccgccgg cgccgcgaga ggcaaggtcg
      361 tctacgagca ggcgggcctc ctccacctcc tcgacccggc gacgaagaag gccgcgccgc
      421 tcccgatcac ggtgaacgcc gacctcctcg agacccggcc ccgctgggcg aagggggcgg
      481 agtgggtccg cgacgcctcg ctctcgccgt ccggcgcgcg cgtcgcgctc gagttccggg
      541 gcgagatcgt caccgtcccg gccgagaagg gggacccgag atacctgacc tccacgaccg
      601 gcgcgcacga gcgcgggccg gcctggtcgc ccgacggcag gtcgatcgcg tacgtctccg
      661 acgaaggggg cgagtaccgc ctcgtcgtcg ccgcgcagga tggcaaggga acgccgaaga
      721 agatcgccgt ccccggctcc ggcttctacg accgcctcgc ctggtcgccc gactcgagga
      781 agatcgcgct caccgacaac tcctggaccc tctggtgggt cgacgtcgcc tccgggaagg
      841 cgacgaaggt cgtctccgag cggctctacg cccctcagaa gacgctgaag gcgagctggt
      901 cgcccgactc gcgctggctc gcttacacgc tcgcgggcgc gacctacatc cgcaccctcc
      961 acgtccacga caccgtcgac gggaagtcgt tcccgctcac cgacggcctc tccgacgtct
     1021 ccgacccggt cttcgacaag ggagggaagc tgatctggtt cctcgcctcg acggacgccg
     1081 gcccggtgcg gaactggttc tccctcatca accaggacgc cgatgtcacc cgcgcgatct
     1141 acgtcgcgac gctgaaggcc ggcaccccct cgcccctcgc gaaggagagc gacgaggagg
     1201 cgggcgaggg agcgaagaag gacgacgccg cgaaggccgc cgacggcaag ggcggcaagg
     1261 acgcgaagaa ggacgaggcg aagaaggagg agaaggcgcc cgagccggtc gtcatcgacc
     1321 gcgacggctt cgacctccgg atcgtcgacg tcccggtaaa ggccgccgac atcaccgccc
     1381 tctcctcgcc gaacgccggc gaggtctggt tcctccgcga ggccgacggg aagacggcgc
     1441 tccagaagtg ggacacgaag agccggaagg ccgagacgat cgccgccgag gtcgacgatt
     1501 acgaggtctc cttcgacggc aagaagctcc tgacccgcgt gaaggactcc ttcgaggtcg
     1561 gccccgccgg aaagaagggc gaagggaagc ccggcgacgg cgggaagctg aagctcgacg
     1621 ccgtccaggt gcgcgtcgac ccgcgcgccg agtggccgca gatcctcgag gaggtctggc
     1681 ggatcaaccg cgactacttc tacgacccgg ggatgcacgg ccgcgactgg aaggcgatca
     1741 aggcgaagta cgagtcgttc ctcccgcacc tctcctcgcg cgccgacctg acgcgcctca
     1801 tccagtggat gtgcagcgag ctcgccgtcg gccatcaccg gacgttcggc ggcgaccggc
     1861 tcgacgaggc gacgcccgtc cccggcggcc tcctcggggc cgactacgag gtcgcgaacg
     1921 ggaggtaccg cttccggaag gtcttcggcg gcctgaactg gaaccccgag ctgcgcgccc
     1981 cgctcagcga gccgggcgtc gaggtgaagg cgggcgagta cctcctcgcg gtgaacggga
     2041 aggagctcgc gccgccggag aacctcttct cccgcttcga ggcgaccgcc gggaagctcg
     2101 tcgagatcac cgtcgggccg aacgccgacg gcaagggctt gcgcaccgtg aacgtcgtcc
     2161 cggtcgcgaa cgaggcggcg ctccgcaacc gggactgggt ggaggggaac ctcgcgaagg
     2221 tcgagaaggc gacgggcggt cgcgtggcct acgtctacgt cccgaacacg gccgggctcg
     2281 ggcacaccta cttcaagcgc tacttctacc cgcaggcgtg gaaggacgcg atcatcgtcg
     2341 acgagcggtt caacggcggc ggcagcgtcg ccgactacta catcgaggcg ctccggcgcg
     2401 agccgatcgc ctggtggacg ttccgctacg gcgaggacat gaagaccccg agcgcctcca
     2461 tccaggggcc gaaggtcatg ctcatcgacg agaccgccgg ctcgggcggc gacctcctgc
     2521 cgtggatgtt ccggaagttc aaggtcgggc cgctcgtcgg ccagcggacc tggggcggcc
     2581 tcgtcggcat cctcggcttc ccggtcctga tggacggcgc cttcatcacg gccccgaacc
     2641 tcgcgttctg ggctcccgag gaaggtttcg gcgtcgagaa cgtcggcgtc ccgcccgacg
     2701 tcgaggtcga gcagacgccg gccgacgtca tcgccggcag ggacccgcag ctcgagaagg
     2761 cgatcgagat cgtgatggag cagctgaaga agagcccgcc agcggcgccg aagcggccgc
     2821 cgttcccgat ccggtaggag aacccgcggc acggcgcgtg cgtacctcga ctcgcatgcg
     2881 ccgtgccgtc ctctccgccg gattcgccct cgccctcctc gcgcccctcg cgctcgcctc
     2941 ccgtcccgcc cgggccgacg ccctcgccga cctgcggggt gccctcgatc gagccccggc
     3001 cggagaaacc gtccgggtcc gggtcgcgat cgcccggacc gacgtcgaga aggagaagcc
     3061 gaagggagag cgaaaggggg aggccgtcgt cgagcatggg ccgacaggtc tctcgatcca
     3121 cctcgacccg gccttcctcc cgaagccggg gtcgaaggcg gagcggaaga agcacgacga
     3181 gaagatcgtg cggctcgagc cgggagaggc gctgaagctc gtcgacccgg ccacggagct
     3241 ccggcagctg ctggacgggg cttccgtcgt ctccgaccgc ccggaaacgt tcgaggggaa
     3301 gtccgctcgg acggtcgtct tccgcgtcgt ccccgacctc gacgacgagg cccggaaggc
     3361 cgtcaagcga tacgacgacg tcgtcacgct gcggctcgac gccgacggtc tcccgtcctc
     3421 gctcgagcgg acgctcgacc tgaaggcgaa gaagctcctc atctcgttca ccgtctcgat
     3481 gcgcgagagc cggcggttca cacgggtggc cggccggctc gtcacggcgt cggcgcgcga
     3541 ggagagccac agctccggcc tcggccagga gagcgcctcg acgacgcgct ggaccgtgac
     3601 gcccctgtag gggcggtggg aggggccgat ccggcgggcc cttcggtatc ctcggccgcc
     3661 ggaaacccga cggacggcgc gcgcgcagcg atgccggccg ggctccagga gggacacgtg
     3721 ctcgaaccga cgatcgaccc cgcgaagctc gacgagtggg cccgcctcct cgtcacccac
     3781 tccatcggcg gcgtgacgcc ggacgaccgc gtgatgatca aaggcgagag gatcgcctgg
     3841 ccgctcatgg agcggatcga gcgcgaggtg atcgccgccg gcggcctccc cgacgtctgc
     3901 ctcgtcgcgc cgaacaacga gcgggggaag gtctggtccg ccgcgatggg gcgcggggcg
     3961 tcggaggagc agctcgcccg cgtccccgag tggcaccgcg agcgctacgc ctcgatgacg
     4021 aagtacatcg aggtcctcgg cgccgagtcg ccttcgagct acgccggcct cgcccgcgag
     4081 gccggcgctg cgctcgcgaa ggccgaccgc ccgttcgccg acctccgcct gtcgaagcgc
     4141 tgggtcatca ccctctaccc gaccccggcg tatgccgaga tcgaggggct cccgctcgag
     4201 gagtacgtcc gcttcgtcgt cgacgcttcg acggtcgatc cccgcccgct cctcgaggcg
     4261 gagaagcggc tcgagccgct cttcgccgac gggaaacgga tggaggtcgt cacgttccac
     4321 ccgatcgaga agcgcgagct gaccctcgcg atgagcctcg ccgagtcgat cccggtcctc
     4381 tcccacggcc tccggaacgt ccccgacggc gaggtcttca cgagccccga cgcctcctcc
     4441 gtcgaggggg agctcttcct cgacctcccc gtcctgaacg gcggcgtcga cgtctcgggg
     4501 atgcacctcg tcttcgagaa ggggcggatc gtccgcttct cggcgctcga gggggagacc
     4561 cagctcgccg cgatcgtcgg gaccgacgac ggcgcccgcc gcctcggcga ggtcgccctc
     4621 ggcatgaacc ccggactgac gcgcgcgctg aagcacccgc tcttcgtcga gaaggtgggc
     4681 gggacgctcc acgtcgcgat cggcgcgagc tacgagacct gcttcgagcg cgacccgaag
     4741 gcgcccggcg cccgcgagcg gctcgaggag ctcgccgcgc gcggcgtcct gaaccgctcg
     4801 gcacagcacg tcgacgtcgt ctgcgacttc cgtcccggcg gctgcgggcg gcgcgtctcg
     4861 atcgacgggc ggccgatcgt cgtcgagaac gggatctggg tcccgggctg accgcctcct
     4921 ggaggccgaa acggatcggg tccgctgagg accgaggagc accgagagat gacgccagac
     4981 gacggacgcc acgacctcgt caccgggaac cgcgagatgg acgaggagca cgccctccag
     5041 ctccagctgc tgcgggagct cgtgcagaac ctcgagtccg gcgacggccc ggcagccctc
     5101 gaggtcttcc ggcgcctcga cggcttcacg aacgcgcact tcctggccga ggagcgcgtc
     5161 atgcgcctcc acgcctaccc cgattccgag gcccaccggg ccgagcacgg gagcttcgtg
     5221 gaggagctga cgaccctgcg cgcgcgcctc tctgccggcg acgtcgacgc ggtgcacgac
     5281 gacgccgacg ccttcgcccg gcgcctcccg gcgcacatcg cccgggccga ccacgccctc
     5341 gccgagttcc tgcgaccgcg acgcggcgcc gtcgcgtgag gccgggccgg gtcagcccgc
     5401 ccggccaaga acgcggacgg cggtcgggag cacggccccg aggaggtagc ccgggaaccg
     5461 ctcccggatt cgctcgacgg cccggtcgga cgaaagcccc ccgaccgtca cgcggcggac
     5521 ctcctccgcc gccgcctcga accaggcgag ctgcgcctcg acgatctccc gcccgcccgg
     5581 cgcgccgtga cccggcacga acgtcgtccc ggccggctcg aggaggagct ccccgagccc
     5641 ccgcttccag ccgtcgaggt cggcgtgctc gaggttcggg tggtagccgt tgaagacgag
     5701 gtctcccgtc acgacgacgc cgaggctggg gaggtcgatc cgcaggtcgc ccggcgtgtg
     5761 cgccgggccg gcggggcgaa cggtcgcccg gacgcctccg acggagagat cggtcggctc
     5821 cgtcacgagg cgcgtcggcg ccgccggcac ggcgtcgaag aagagcgcgc gcgtcgacgg
     5881 ggaggtcctt cgctccgcga ggagcgccag ctgctcggcg gccatccgcg ccgccgaggc
     5941 cgcgtgggcg atcacctccg cgcccgcctc cgcgaagacg gacgcgccga cggagtggtc
     6001 cgtgtggtgg tgggtcgtca cgacccaccg caccggcccc gccccgcgcg ccgcgaggag
     6061 acgcgcgagc tcccgcgcgt agacgggagc tacgagcggg tcgacgagga gcgtcgcgcc
     6121 gtcgccgagg atcgccacgg cgttcgcccc ccagccggag gcgaggtcct cgcccccggt
     6181 cgagaagacg acgacccgct cgttcacgac cgtctcccgg agcggagaga ggagcgggcg
     6241 cggcagggcc gccacgaccg cgtcgccgag ccaggcgcga gcgtcatcgc ccgtcaggcg
     6301 ccgcagctcc tcctcgtcct cgctcccgtt cgcgaggtac tcgtaccggc tctcgtacga
     6361 gtaaagcgtg ccgcagagcg ggcagagccg cggcgaacgc ggctcgtcct cccgcccccc
     6421 gacccgcact tccacgaggc gacggtacgc agccggaagc aggcggtcgt tctcctccca
     6481 cccgaacttc atgagcgacg tctcgaccgg cttcagctct cggcagatct cgcaggagac
     6541 ggggccggag tccatcaccc gaatcgtacg acgcggcgcg ggccggcctc gtgcgccgag
     6601 cgtcggcgac ggtacgatcc gccgcacaag gagccgccat gaccctctcc tccgcgctcg
     6661 cgcgggcggg gtggggcgtc ctcgcgggcg ccctcctcct cgcctctccc ctcgccgggc
     6721 agggcccgga cgacgactac gacctcgtcc tcgagtgccg cggcgcgctc tggagggcca
     6781 ccttcgacga cgccacggcc gccggcctcg acccgggcgc cgcccccgtc tccttcgacg
     6841 gctcgggggc gcgacggacg cggggcgacg gcgtcgtgac gttccgcgga tcggaggagt
     6901 accagccgtc tcaccggacc cgggccatcc cggtccggta cgagtgcgtc gtcgacacgg
     6961 cgacgcgaaa agtcctctcg atcacctatg aggccgtcga cgcctcgggc gctccaatcg
     7021 cgaagcgtcc cgtcgacctc gtccgcgacg ccctcctcct cggcgcctgc cgcgagtcgc
     7081 tcgaggacga ggtgcgcgac gacgcccgcg ccggcggcgc gagcaccgac ggaaacgacc
     7141 tcgagcccga ccccgcctcg gtcgcgcgga cggagaaggg aaagctcgtc gagctgaccg
     7201 gccggggccg tgcccggctc agcaaggact acgagtggga ggcggtcacc ttcggctgcc
     7261 gctgggaccc ccgcaaggag gaggtgacga aggcctggta cgcaccggtc ggcgcgcgaa
     7321 agctcgggct cctgacgccc gagaagcggg aggctctcga cgcctgccgg cgcgcgattc
     7381 gcgacgccgt gtgggacgac tcggccgccc gcggctaccg gtggccccgc gaccaggtcg
     7441 tcgtcgagct ctcccgccgc ggcgacttcg ccgcctcggg cgatcgcgtc gaggtgaagg
     7501 gcgaggggtg gttcaagtcc gacgcgcgcc actcgcagtc gacgccgatc gccttccgct
     7561 gcgccttcga cgcgaacctc caggccgtcg tcgacgcctc cttcgaggcg ctcgacgcct
     7621 cccgcactcc cacgggcgag atcgcctcgg ggaagaccgg cacgctcgtc tgcgagtcgt
     7681 tcgggaaggt gcagaaggtc tgcccggcct cgatccgcgg gaacgtccgg gtgatccgcc
     7741 agctcagctc cgtcgcctgc aaggcctacg agaactggat ctacagcctc tcggggatca
     7801 cggtctgggg cggctgccgc gccgagttcg agttcgacgc ccgatgagcg aaggagcgcc
     7861 tccgcccgcg gcggcggcgc tccttctccc ttccgtccct cgtcaccgca ccgtagcgcg
     7921 cggcgcttcc ttcgcgggcc gcgacccgcg cagcgccgcg agcgcggact ccagccgcgc
     7981 gagctcgcgc tccatcgccg ccttctcggc tcccgcctcc ggaagctcgc cgcgcagctc
     8041 cccgacctgc tcggcgagcg agcgctcgag gtcgtccgcg gccccccgga gcgcgtggac
     8101 gcgcgcctcg gcctcaagcg cccgcgcctc gcgttcgagc ttctcggact ccgcctcgag
     8161 gagctgctcc tgcctgccga gctccgcctc cgcgcgcgca gcctcgcggg cccgcgcctc
     8221 ggcatccctc tccgcgcccc cggcttccgc cgcgcgctcc gactcctcct cgagcttcag
     8281 ctcacggagg tgaagctcga gtcgccgccg ctcgacgtcc gcctggcggg cgcgaagcgc
     8341 gccgtgcttc tccgcacgcg ccgccccggc cgcgtctccc tcggcccgga ggcgcgcgac
     8401 ctcggcggcc tgctcctgcc ggtccgcccg ggcctcgagc tcgagggcgc gccgctcgag
     8461 gtccagccgg gagaggtcga gctgccgcct ctgcgcgtcg agcgcacgcc gctccctctc
     8521 gagctccggg tcgggctcct ccccggcgag caccggatgg acgtcgggcg ccggcgcggg
     8581 atgcgcggcg atgcgcggcg ccggcatcgg cgccgcgtgg ggcgcgggcg gaggcggagc
     8641 cggggccgcc gccggcgagc gtggggcgac ggcgacgggg cgcgcgggcg ccgcgacggg
     8701 tgacgcgggc gccggtgggg gcggaggcgg aacctcgtcg ggaaggcccg ccagtgccgc
     8761 ggcctccgcc gcggccggcg gcgcgaccgt cgggaagaac gcgagcgcgc cgacggcgag
     8821 acagccgagg gcgaggcgga tcgggcggga gagccggggt cgcggtgacg tcatgaggat
     8881 ctccttcagc cgggactcga ggtcccggat cggtccggcg ccggacgcgg tctccggaag
     8941 ggcgacgggc ccctccgcga ggaacgtcag gctcttcagg attccgctcg cgtaggcgcg
     9001 cgccgaggcc gggaacgcgc gaaggaccca ctcgtcgcag cagcgctcct cggcgcggcg
     9061 aagctcgcgc cggctccacc aggcgaccgg gtaccaccag aagagcgccg tcgcgccgat
     9121 ctccaggaac ctcacccagt ggtcccgccg cctcacgtgg gcgagctcgt gggcgagcag
     9181 cgcgtcgagc tcgtcgccgc ccagctccgg gaggaggtcg cgcggcagga gcagcttcgg
     9241 acgcggggag gccggccaga gcatcggcgg gacccgggcc gggaggaggc agaccgcggg
     9301 agcgcggcga acgccgaggg cggcgccgat ctcggcggcg cgcgtcacga gcgcgggcgg
     9361 agccggctcc gccgcctccg cgaggcgccg aaaccggagg aagcgccgcg cggcgagcgc
     9421 ggcgacggcg agcgcgccga gcccgacgat tgccgtcagg gcgagacgcc agggcaacgg
     9481 cgccgcgacg gcgcgggcgt cggccggggg cgcgcccggg agcgctcctc gcgcgcttcg
     9541 aggcccggcg gatccgggag cggcgtcggt gcgcgccgcc tcgacgatcc gtgcccgcgg
     9601 cgcggcgaga ggctccgggg cggcggggcc ccggacgtcg gaggggacgg aggaggtcgc
     9661 ttcctccgcc cgcggcacgg catcgcccgc cggaaggagc ggcagctcga cgagaggcgg
     9721 agtcacgagc ttcagaaggg ccaggaggaa cagcccgtgt acgaccgcct gccgcttcgt
     9781 gaagcgcgag acgacccagg cggcgagggc gaggaggccc gcgaggaccg cgttcgtcag
     9841 gaggaggcgg agggcctcgt gcacgccgtc acctctgctc gccgagcgag tccacgaggc
     9901 gccgcaggtc gcgtagctcc cgggcggaga gccgcgctcc gctcacgagg tgcgtgagga
     9961 gcggcgtgaa cgagccgccg cagagcgagt ccgccgtctc gcgcagccgg agcgcgacga
    10021 ggtcctcccg gccgaggcgc gcggcgtaga cgttctgccg cccttccgag cggtgcgaga
    10081 cgtgcccctt gtcctccagc cgttcgagca gcttctggac ggtcgcgtac tcggaggcgc
    10141 cgccgttcgg gtagacccga tccgcgaggg cccggatcgt cgcctccggg aggtcccaga
    10201 gcgcgcgcag cacctcgagc tccgcgtccg tcacgtctcg gggggttcgc gccatcggat
    10261 cctcctgtcg tcccgccttc tcagacgtta cgtctgagaa tctgagacat cccgtctgag
    10321 aagtcaagga ggacgacgaa gaaagcgcgc gacgaacctc acggcgggtt cgaaggcccc
    10381 ggactactcg tgcggagtcg cctccgacca gccggcgacg gcgaacgggc agcccccggt
    10441 ccgccggacg acgacgatcc ggcccgtccg cggaagcgtc agcggcgtcg acggcaccgc
    10501 gaagaagcgg ccgagcgcgt ccgcgaacgg aatccccgcg atcgagaccg accgcgcgtg
    10561 ccgggcgcgg gtcggcaggc cgccggccgg gacgagacgc gcctcgatct gctccggcct
    10621 cggggcccgg gcgagggaga gcccgttgag gaggagcccg gcgagcccgt cgagcagctc
    10681 gcggccgacg tcccgggcca caccggagaa atcccgtcgc gggtgggcga ggcgctcggc
    10741 gtcgcgccgg agcgacgcga ggagctccgc gaccgcgccg gccgcggcat tcgcgacggg
    10801 ctcgggaagc cccgccggga gcgggacgct ctccgtacgc ccgtcgacgt cgacgtcgac
    10861 gtcgcgcgcc ccggtctcgc ggaaccacgc gaagacctcg accgacccgt ccaggacgcc
    10921 cgggggaaag ggcggcagct cacgggcgag acggagcgcg agagcgacga gcccccggcg
    10981 gaagcggacg aagacgtcga cgtagacgag cacgtcccgc gggtcgggac gcgcgcagac
    11041 gtgcgcgagc gccgcgagga gcgacgtccc ctcgccggcc agcgcccgct ggagcggccc
    11101 ggtgaggacc ccgttcatcg gatcgtcgag cgcgaggcgc gccgcaaccc cgagaacctc
    11161 gaggagcccg ccgaggtccc gcacgccgtc tcccgccgcg cggagctcgt cggcgacggc
    11221 ccgcacgtcc cccggcgaga gcctcccgag gagtacgttt cccgcgagcg cgagccggac
    11281 ggccggaatg agcccggccc ccgcgaagtc gacggtctcg cggtccggcg cgagcggccg
    11341 cccctcggcg tcgaagagcc tccgctcgaa gacgagcgtc tcctcgagtc ctcccgcgcc
    11401 ggcgaggcgc cacggatgag acccgcgaga ccgcggcgcc gggaccgcct cgctcatccc
    11461 ccgccccggg gagaccggga cggagccccg ggtccgttcc gtccgcgatc gaggaaggcg
    11521 tccggctgcg gattcacgtg ctcctcctcc cggccgcgac ggccaccggc gatccggctc
    11581 ttcgacgatc cgtttcccaa acgccctacc ggaagtaccc gttcacgtcc aggatcaggt
    11641 gcgccgccgc agccgcgccg ttgcggacct ggatcgtccg cgtgccgtcg cgggcgagct
    11701 cgatcacggc gttgttcgcg cgcgtgcgcc ccgcggcgaa gctcacgttc gacgtcccgg
    11761 gtaggtcgcc gtctccggcc cagaggacga agctccccgc ggccccggcg gcgacgaccg
    11821 tcacgttcgc cgagatcgca cgcgcgtccg gcgggatgcc acaggtaccg ccgagcggga
    11881 agaggcggcg cgccccggcg gagatcgcgg gcccgccgag cgggccggcc gcctcccgcg
    11941 tgtcgaagag ccggcacggc gtgagggcgt agaaccgcga ggagcgcggc ggccccgcgg
    12001 agcccggcgc cgcgccggtc cggcggagcc cgccgcgccc ggtgccccag aggacgcgcg
    12061 cgttcgaggt ccccgggagg tcgtaggcaa ccgcgcccga ggagaccgtc ccgacgacga
    12121 cctcgagctc gccgtcgccg tcgacgtccc cgagcgacgg cgcgccgagc gccccgttcc
    12181 aggtggcgct gccgtgcggg gcggggaggc tgacggcgtg gagggagacg ccgaggtggc
    12241 tcaggacgtg aagctggccg gttccgccca cggacttctg cggccaggag gtgaagatca
    12301 cctcgcccgt gccgtcgccg tcgaggtccg cgacgaccgg ctcgctcgcg aagcggatgc
    12361 cgctcccggg gacgtcgtag ggccagctcc cctgctccgt cttgtcgagg gaccaggcat
    12421 gcaggcgacc gtcgtacgac gggtagagga tctccttcct cccgtcgccg tccaggtcgg
    12481 cgagcgccgg gttcgggacg gccgtctcga tgacgtcgta gtcctcggag agcggcccgc
    12541 ttccggcggc cggcgtcgga agcaccgtcc agtcccaccc cgcgccagtc gcccgcgtcc
    12601 ggtccagccg gaagaggaag ggcatgtggt agaggctcgt gtacgggttc gtcgcgcagt
    12661 tgtagacgtt cccgacgatc acgacctcgc ggacgccgtc cccgtcgagg tcgccgatga
    12721 cgggggccga atcggcccag ttcgggcggt gctcgacgcc gcagttcgcg tagccacgga
    12781 ggtcgaccgc gtggtcgacg tggacgccga cctggctcca gaccttcccc gcgccgtaga
    12841 tcgggctggc ggggagctgg ttccctccgc cgtcgagcgc ggtgacgtag tgcgtgtcgg
    12901 tcgggccgat cagctccttg tcgccgtcgc cgtcgaggtc cgccaccgcg acgttctcgt
    12961 tgtacatccc ccagccgtag ccgggctcgc cgtcccgccg cgcgggccac ccgctccgga
    13021 cgttgccggc cgcgtcgtag acgttgagct ggcgggtcga gcccccgctc gcgcgcccga
    13081 cgacgacctc gaggaccccg tcggtgtcga ggtcctccac ggcaagcgtc ctcacctcgc
    13141 cgccgccgaa cgggttccgc gtccagacga cgccgccccc ggcgtcgtag acggtcagct
    13201 ggtccgagcc ccggccgacg accacctcga gcgtcccgtc gccggtgagg tcggccacgg
    13261 cgacgccggg ccagacccgc gagccgttcg tcgcgcgcca cctcagggcg cccgtcgcgc
    13321 cgtccagggc gacgacgtcg tacgagcccc agacgacctc gggcgcgccg tcgcggtcga
    13381 ggtccgcgat cgcgggcgag gagtaccagc ccgtctggca ccacgacgag aagcagcctc
    13441 ccctctgcca cttcaggacg ggggcggcga tcgccgaagg ggagctctcc tgcgcgccgg
    13501 ctcgggaagc gacgagcgcc gggaccatga ggagcgcggc ggcgacggta cgggcgacga
    13561 cggttcggta gtgggccatg acctctcccg cgaacggtcg cgcggtgggg ccgaacccgc
    13621 gactcttccc cgggagctta ccgccgggcc gaaggcccgg cggtgaccga acggcggagg
    13681 acgcccccgc cgcgcctcag agagccagcg tcttcgcccc cgccgcgagc gtcgccttca
    13741 cgacctcgag gagccgcgcg ggcggcacgc cctcggcctt cttctcgcgg cgcgtggaga
    13801 cctcgacgac ccccttcgcg agggccttgc cgccgacgac gatgcggagc gggaagccga
    13861 tcaggtcggc gtccttgaac ttcacgccgg ggcgctcgtc gcggtcgtcc cagaagacct
    13921 cgacacccgc ctcggtcagc tccgcgtaga tcttctcggc ggcctccagg acctccttgt
    13981 cgcgctggag cgagaggagg gcgacctgga acggcgcgag cgggacgggc cagatgatcc
    14041 cgtcgtcgtc gtggttctgc tcgacggcgg cggcgaccgt ccggccgacg ccgaggccgt
    14101 agcagcccat ctgcagcggg accatcttct gctgcgggtc ggccacctcg cacttcatcg
    14161 tctcggagta cttcgtcccg agcttgaaga tgtggccgac ctcgatgccg cgcttgatcc
    14221 ggaggacccc cgtccccgtc ggcgacgggt cgccgtcgcg cgcggtcgcc acctcgatca
    14281 ccccggccac cttcaggtcg cgcggaacgg agaggttgga gtggtgggtg ccgtcctggt
    14341 tcgcgccgca gaagacgttg cgggcgttct ccatggagcg gtcgacgtag accgggacct
    14401 ccgcctgccc cgcccggggc ttcaggccga tcgggccgat gaagcccggc ttgcagccgg
    14461 cgatttcggg gagccactcc tcggggacga ggcgcacgtg cgcgacgccg agcgccttcg
    14521 ggagcttcac ctcgttgatc gtcaggtcgc cccggatgac gatcgcgcag acgaacgagc
    14581 tgtcgtcggc gcggatcgcc tcgtagagca tcgtgttcgt gatccgatcc tcggtcgtcc
    14641 cgaggtgctt cgcctgcccc tcggtcgaga cgatccccgg cgtcggcgag tcggcacggc
    14701 cgaacggctt ctcctcctcg ttcgcccagg gctcggggag cggcgccgtg acggccttct
    14761 cgacgttcgc gccgtacttg ccgtcctcgg agaagacgag ggcgtcctcg ccggtgtcgg
    14821 cgacgaccat gaactcctcg gaagcggagc cgccgatggc gcccgagtcg gcctggacga
    14881 gcgcgtactc gaggccgcag cgctcgaaga tcgcccggta cgccgtgtcc atcttccggt
    14941 agtgggcgtc gagccccttc gggtcgacgt cgaaggagta ggcgtccttc atcaggaact
    15001 cgcgcccgcg catcaggccg aagcgcgggc ggatctcgtc gcggaacttc gtccggatct
    15061 ggtagaggtt gaacggcagc tgccgccacg acgtgaccga ccggcggacc atgtcggtca
    15121 ccgcctcctc ggccgtcggc gcgagcgcga actcggtggc cttgcggtcc tcgaggtgga
    15181 agaggatgcc gtcgttcatg tagcggtccc accgccccga ctccacccag agctccttcg
    15241 gctgcgcgat cggcagctcg acctcgagcg cgccggtccg gttcatctcc tcccggacga
    15301 tctcggtcat cttcttgagc gaccggaagc cgagcggcgt gaaggagtag atgccggcgg
    15361 cggccttcaa gagcatgccc gcgcgggcga ggagcttgtg ggagacgacc tcggcatcgc
    15421 cgggggcctc gcggagggtg gagaggaagt agttggacca gcgcatcggg ggcggagaat
    15481 agcgcagcgg agcgggttcc gttcccggta cgatcgccct ccggcccggg cggacgaccc
    15541 gaggccgcgg gaggcctccc ggatggacgc ccagccgttc gacttcgttc ccgtcgcctc
    15601 gcctcttcac gacgccgcgg ccctccggcg ctcgctcgag ccgtacctcg gggccctcgt
    15661 cggcctcggg ggccgcgaga cgggtccgga ggccgccacc ggatccggcc cggtcgcggt
    15721 cgtcgtcggc accggcggca ccgaggccgc cgtcctccgc cttcgcgagg cgcgtcaggc
    15781 cgtcgccccg gacgagccgc tcctcctcgt cgccctcccg gcccacaact ccctcccggc
    15841 cgccctggag gcgctcgcgc gcgtgcggca ggacggtgga cgcggccgga tcgttcccct
    15901 gcgcggcccg gatgacgggc ccggcctcgc ccgcgtcgcg gccgccctgg aggacctgcg
    15961 ggtgagggag cagctccacc gcgcccggat cggcctcgtc ggagtcccgt cggactggct
    16021 cgtggcgagc gtccccgccg catccgccgt ccgcgagacg tggggccccg aggtcgtccc
    16081 ggccgagctc ggcccgctcc tcgacgcccc gctccgcccg gccgacgatg cgaccgccgg
    16141 cctcgctgcc gctttcacga ccggggccgc caccgtccgc gagaccgacg aggcgggcgt
    16201 gacgaccgcc gcgagcgttc tcccggcgct ccaggccctc gccgcgcgcg agcggctcga
    16261 cgccgttgcc gtccgctgct tcgacctcgt caccgagcgc ggcacctccg gctgcctcgc
    16321 cctctccgcc ctcaacgacg ccggcaccgt cgccgcctgc gagggagacc tcgcgagcgc
    16381 cgtcgcgatg ctctgggtga aggagctcct cggcaccgcc tcgtggatgg cgaacccctc
    16441 cgacgccgac gccgtgacgg gccgccttcg cctcgcccac tgcaccgtcc cccgctcgct
    16501 cctctccggg tgggacctcc gctcccactt cgaatcgggt ctcggcgtcg cgctcgccgg
    16561 cgcgctcccg cccgggccgg tcacgctcct gcgcctcggc ggccgccgcc tcgagtcgct
    16621 cttcctcgcc gaaggcgagg cgctcccgac gccgcgccgc gaggacctct gccgcacgca
    16681 gctcgacgtc ctcctcgagc ccgccgccgt cgccgagctg ctcgaacgcc ctctcggcaa
    16741 tcatctcgtc ctcgtccccg gccacgtcgc cgcgcgcctg cgggcgtacc gcgagtgggc
    16801 ggtgggaggg gaggacggct cgccggcggc cgcctgaccc gctgataggc tctcgggatg
    16861 gccgttcgat acgtctccga ggagccgcac cgcgtcgctc cggagcacgt cggcgccgag
    16921 ctcgcgtcgc ccgcccggcg gggcgtcgcg ctcgcgatcg acgtccccct cctcgcggcg
    16981 ctcctcgccc tcggatgcgc tctcgccgcg gcgccttcgc gcgcagcggc cttctgcccg
    17041 gcgttccctc ccgccacggg cgacgtcgtc gtcgtgagca cggtcgcgca gctgcggacc
    17101 gcggtcgacg cggccgtgcc ggggcggacg atcctcgtgg ccgacgggaa ctacgcgctc
    17161 aacggcgagt acctccgcat cgacgtcccg aacgtgacgc ttcgtgggca gagcggaaac
    17221 cgggacgccg tcgtcctcga cgggaactac ctgacgaccg agatcgtcca ggtggccgcc
    17281 tccgacgtca cggtcgccga cctgacgctc cgcgaggcgt acgaccaccc gatccacgtg
    17341 atgccggcgg gggcgtcggc gacgaaccgg acgctcgtct acaacgtccg catcgtcgac
    17401 ccgggccagc aggcgatcaa gatcaacccg acgagcgaca ccgggccgta cgtcgacgag
    17461 gggacgatcg cctgctcgcg gatcgagctg accgacgccg gccgggccca cgtgagagac
    17521 aactgctaca ccggcggcgt ggacgcccac gccgcgctcg gctggacggt gcgcgacaac
    17581 gtcatcgagg gtttctggtg cgacgcgggc ctctccgagc acgcgatcca cttctggcgc
    17641 aacgcgcgcg acacgcgggt cgagcggaac gtcctccgca acaacgcgcg cgggatcggc
    17701 ttcggcctcg ccgagaccgg cactccgcgc cggacgtact ccgacgctcc ctgccccgcc
    17761 gcgggaggcg cctacgtcga ccacttcggt gggaccgccg tgaacaactt cgtctccgcc
    17821 gacgacccgg acctcttcgc gtcggagtac ggcttcgact gcggcatctg cctctggcag
    17881 gcgtgcggcg cctccgcgct ccacaacacc gtccacacgg ccgacgtcgc ctcgtccttc
    17941 tccgcgatcg agtggcgctt ctcccgcacc accgcgacca tcgcgaacaa cctcgcgaat
    18001 ttctcgctcc gggcgcgcga cggcgcctcg gcgacgcagg cgacgaacgt gacgaccgca
    18061 acggccgcgt tctacgtctc cccggccacc ggcgacctcc acctccagcc gacggccgcc
    18121 ggggcgatcg atcaggggag cgcctcatcc gtcgggaccg acttcgacgg tgactcgcgc
    18181 ccgcgcggcg ccgcgcctga cgtcggcgcc gacgagaccg gtgcgccgca ggcgctgcgg
    18241 ttccacccgc tcgcgccctg ccgcgtcgtc gacacccggt cggcttcgct cggagggccc
    18301 gcgccgctcg cggccggcgt ctcgcgcccc ttccaggtct ccggagcctg cggcgtgccg
    18361 gccggcgtga ccgtcgtctc ggcgaacgtc acggtgaccc aggcgaccgc gtcgggaagc
    18421 gtcgtcgtcc acgccaacgg gacgcccgtc ccgatcacgc ctcaggtcgc cttcgccgcc
    18481 ggccggacgc gcgccaacaa cgcgatcgtg ggcctcgacg gcgccggcct cgtctcggcc
    18541 cgggccgaga tggcctccgg ctccgttcac ctcatcgtcg acgtgagcgg ctatttcgac
    18601 tgagagcgcg gcccgcccac agcgcgaccg cgccgttgcc tcgcgcggct cgcggtcata
    18661 gactcgagaa gctcccgtgc gacttctccc cctcacgctc ctcgccgccc tctcgctcgc
    18721 ccggcccgcc gacgccggcc ttctcgacgt cgcgaccggg gcggtcgccc ccggcgacgc
    18781 gatcgccgtc gcgtggaccc tccctcccgg attcgaggag agcgagctcc tcatccaggt
    18841 ggacggggga ccgcgcatcc ggctcacgcc ggagtgccac gacgagaatc cccgtttcgt
    18901 catagaggtg ccggcgctcg ccggcagggc tcggttcgtc cttcgagccg gccgggtcga
    18961 agaggacggg gtccacaggg agcacacgat cgactcgacg gagtggttcc agatcgcgcc
    19021 gtcgttcgcc gcgggccccc tccccatccg cgctccgcac acccatccga gcgagggcga
    19081 ggagatggag tggtggtcgg actcggtcgg acgggtcccg gaaggcccga cgtcgggcct
    19141 tcatcgcccc tggaccgctg aggtctccgc cgccacaccg tcgtcgcccg cgctctccga
    19201 gagccgctct gaagaccttc tgtcttcgga gtcctcggtg cgcggcgacg tcgacgcccg
    19261 agcacggaat cggaacgggc tcgagcgagg gtatcgagag cgctccttcc ccggggcgcc
    19321 ggtcccgctt cggaattgag cccgggaaga gcctgcgcag acggagcgtt ccgtcccgtc
    19381 cgtcgctcgt cgcgtcggtg acgggacacc gtcccggccc gtggctttct tccccccctc
    19441 cgcgctcgat tcgaaggaaa ggatcgtcga atgccctgcc gttgcccctg tgagatcggc
    19501 tcgtcccggt ccgttcgtgc ggtgcttctc accgcgctcc cgctcctcac tctccttctg
    19561 tcccccgtcc tcgaggcggc gcgctatcgc gtcgtcgacg agaagggccg cgccgtcccc
    19621 ggcgcccgcg tctccatcct cggccgcgcc ggctccgtcg ccgccggtcc gcagggcgag
    19681 ctgacgctcg acgccgagcc cgccctgccg ttcgaggtcg gcgtcttctc gccgaccggc
    19741 gcctggctcg ggatcgtccg catcgtgacg aagagcggcg cggacgaggt ccgcgacctc
    19801 gtcctccccg cggcccgcag caccgaggtc ctcgtctccg cgggcgccac cgcctcgctc
    19861 ctcgccccgc ccgcgaaccc ggtctccctc gtctcgaagc gggagctgga ggagcgccag
    19921 ccgaaccgtc tcgtggaagc catcgagacg ctccccggca tctcgaagag cgacgagtcg
    19981 acgagcgccg tgccggtcat tcggggcctc gcccgcggtc ggacgctcgt gatgctcgac
    20041 gacggacgcg tctccgccga gcggcgcgcc ggcgcctcgg cctcgttcct caatcctttc
    20101 gccctcgaat cggtcgaggt cgtccgcggc cccggctccg tcgcctacgg gtccgacgcc
    20161 ctcggcggca tcgtccatgc ccggacgccg ctccccgagc cgggcgtctt cggcgtccgc
    20221 tatcaggtca gcggcggcgt cggcgggagc cccgaggcgt ccgccggcgt cggcctgaac
    20281 gtccccgtcg gcccggcggc attcctcgtc aacctctacc agaggaacgc aggcgactac
    20341 gagtcgcccg tcggagagca ggacaactcc gctgtccgcg accgtggctt cctcgtccgc
    20401 ggcgcgatcc cgatcgggaa ggcacgcctc ttcgccggct tccagctcga cgaggtccgc
    20461 gacatgggca agcctcaggc cgacctcccg acgacgcgga cgttctaccc gtgggagaac
    20521 tcgcgccgct tctcgctcgg cctcgacgtt cccgacgtcg ccggcttctc ctcgctcgag
    20581 ctccgcgcgc tctacggctc ctacggcatc tcgacgaacc gccagcgccg gccgaacgag
    20641 acgacgacgc ttcgcgactc catctccgag gtcgacgccg acgacttctc cgtgcgcctc
    20701 ctcggccgac gctcgctcgg gcccgtgggg ctccgcgtcg gcgtcgacgt gaacggccgg
    20761 acgggcctcg aagcccggaa cgtgacgaac aactacgacg tcggcgggaa tccgacgagc
    20821 tcgacgttcg agatcgccgt cgaggacgcc tcgcgcaccg acttcggcgc cttcgtggag
    20881 ggagacctcc ctctggcggg agaccgcctc atgctaacgg ccggcctccg cggcgaccag
    20941 gtgaagacgg agaacaccgg cgggtactac ggcgaccgct cgacgtccga cggggcgctc
    21001 tccggcttcg cgacgctcac gtacacgatc gcgcccgagt ggaagctctc cgcgcagtac
    21061 gcgcgcggct tccgcgaccc gacgctctcc gaccgctact tccggggtgt ctccggccgc
    21121 gggttcgtcg tcggcaaccc cgacctctcc gccgagacct cgaaccagtt cgacctctcg
    21181 cttcgcggac gcaagggcat cttcggcttc ggcgtctccg gctacgtcta ccggatcgag
    21241 gacctcgtcg agcggtacaa gtcggggaac gacaacctgt tccgcaaccg cggcgagacc
    21301 gagatcgcgg gagcggagct cgaggccgac gtgaggatca cgccgcgcct cgtcgtccgg
    21361 ctcctcggct ccctctccca gggacgcatc ctcgacgacg acacctgggc cgccgacgtc
    21421 ctcccgccga cggcgcagct gatcgtcgac cacgagccgt tcggcggctt cttctggcgc
    21481 gcgcggctca tcgcgatggc gcgcgacgag agacccggcg cgagcgaggt cgtcgtggcc
    21541 ggacacgccc ggctcgacct cgccgccggc taccgtctct ccgacctcct gacgatcacg
    21601 atctcgggcc ggaacgtcct cgacgcgagc tacgccgact cggccgacga gaacaacgtc
    21661 ctcgcgccgg gacgcggcgc gatcctctcc ctctcgggct cgttctagct ccgttcgtat
    21721 ccgggaccgg gtccctcgcg aaggcgcgag gggccccgtc cgggagcggg ttcgacgacc
    21781 cgaacgtcga aatcttgcgc gaagggctcg ccagtcccta tcctccgcac cacattccga
    21841 tgccgctcct ccgccgcccg cgcgcgctct acgcggcgtt cgaccgcttc ccgacacgga
    21901 agggggcgtc gacgcacatc gcccgcttcg ccccggcgct cttcgaggcg ttcggcgggg
    21961 gactcctcta cgtcctcggc gacgagcggc tcccggcgca ccaggtcgag ggagaggtcg
    22021 aggtcgtccg cttctccgtc ccggtggaga acttcctcga acgggccgtc gcgttcggcg
    22081 cgcacctctc gcgccttctc gacgagtcgg gcgaggcgct cgaggtctgc cacttccgcg
    22141 acccgtggtc cggcgtcccg atcctcgagc gggagcggcg ctacgcgacg ctcttcgagg
    22201 tgaacgcgct cccgtccgtc gagcttccgt atgcctttcc gtccatcgcg ccgcggacgc
    22261 tcgagaaggt cctcgccgcc gagcgtttct gcctcgacgc cgccgaccgc gtcgtcacgc
    22321 cgtcgcggac gacgcgcgac ttcctcgtgg cgcagggcgt cccggcggcg aaggtcgccg
    22381 tcgtcccgaa cggggccgac ccgatcccgc ctcggccccg gccgttcgag gcgcccgacg
    22441 cctacctgat ctacttcggc gcgctccagc cgtggcaggg ggtcgacacg ctcctccgtg
    22501 ccttcgcgcg cctcgccgac ctgacggagc tgcgcctcgt cgtctgcggg tcggcgcgct
    22561 cgcggccggc ccgcgccgcc gagaagctcg cggagaagct cggcctcgga gagcgggtga
    22621 cctggcggtg ggagctcccg acggccgagc tcgagccgtg gctcgcgaac gcgctcctct
    22681 ccgtcgcgcc gcttcgggcc acggagcgga acgtcgtcca ggggtgcgcc ccgctgaagg
    22741 tcctcgagtc gctcgcggcg ggcgtccccg tcgtcgcgtc ggacctgccg cccgtccgcg
    22801 agatcgtgac ggacggcgtc gaggggcggc tcgtcccgcc cgaccgtccg gccgacctcg
    22861 cgcgggcgat ccgcctcctc ctccacctcc ccgaagagcg ggagcggatg gcggcggcgg
    22921 cccgcgcccg cgcggcacgc gacttcggct gggaccgctc cgtcgcgctc ctgaaggacg
    22981 tctaccgcgc gctgcggccc accgccgcga ccccttccgc cccccgcccg atcgaggagg
    23041 aaccggatgt tcgaagcgct gaaggcccga aagtcgctgt cgcctgagca ggtgacgctg
    23101 ctccagacga agcagctcgc cggcgagtac gagccgggcg cgctcgtcgc gctgctgaag
    23161 ccgatcgccg agtacgaccg gctgagcgac aaggcgcgga cgccgatggg ctgcacgacg
    23221 ggagcgctct tcgtcctcgc gttcgtcctc ctgatcgtct cggcgaacgt ctccttctac
    23281 ctcctgccgc tcccgctcgc ggccctcggc gcggggatcg cgctcctcgt cacggtcctg
    23341 aagatgaaga agctcgacct ctcgaacaac ttccggcagg tcgcgatgcc gctcttcgcc
    23401 gtcctccggg aggacatgga gccgggcgcc acgatgaacg tgcgcctcga cctctcgccc
    23461 ccgacggcga aggggaagaa gatcggcacc gggaagccgt acgacgtccc cggctatcgg
    23521 aaggtcgtcg acacgaccta cgtcgacccg tggttcggcg gcggggcgcg gctcgccgac
    23581 ggaagcgtcc tccagtggac ggtcaccgac gacgtcgtcg cgagcgagag gacgaagcgg
    23641 acggcccgcg ggaagatcaa gacgaagacg aagcaccgga agctgacgac gatcgccgtc
    23701 gacgtcgcgc tccccaagaa ggagtacgcg gtggcggcga tgccggccga cgagggggag
    23761 aaggtgaagg tgaaggacgg cgagaagcgg acctcgctcc acgtcgagaa gagggtgaag
    23821 acgaagtcgc tcgacccgat cgacccgagg gccgtcttcg acgccatcgc cgaggcctac
    23881 aggcgcgtcc gcccggcggg cgccgagggg agcgcgtcat gagcggctgc gagtgcaagg
    23941 gcgggttctt cacgctccgg gactgcgacg gggcgcccgc gggcgcctgc tcggagtgcg
    24001 ggcgcaccgc ctgcgcgcgc cacctctccc ccgcgagcgg cttcacgcag tgcctcgact
    24061 gccacgcgcg cgcggagcag cagccgcgtg aggggcagaa cgcgaccgga gaagaggtgc
    24121 tcgacgacga gtggacctac ggtgcgcggc accgcttcta cacggccggc tacgcccctc
    24181 tctatgccgg ctcccaccgc agccactact acgacagcta cgacacccgc tcgttcgacg
    24241 acgagggagt cacggaggcc gagaccgacg aaggcggggc gggcttcgga gacagctgag
    24301 gtgcggccgc tcgtcctgac cgagaactac cctcccgacc gcgggggaat ggcgcagtcg
    24361 tgcgaccgga tcgtgagggc gctccgcgcc tcgggcgtcg acgtcgacgt cgcgcacctc
    24421 ggccggcgcg ccgtgcgccc gttcgtcgag cggcaggagc gcggcctcct cctcgcggcc
    24481 cccgccgacg aggacccggc ccacgcgacc cagctcctct ggaacgaggt cgcccgcctg
    24541 ccacaccggc cgacgcacgt cgtcgctttc ggcggcgcct ctcccgtcct cgccgcgccc
    24601 gtcttcgcgg cctggctcgg ggtaccgctc gtgacgctcc tgcgcggaaa cgacttcgac
    24661 accgggctct tctcgctccg gcgcggctgg gcgctccgcg acgcgctcgg ccgctcagcc
    24721 gccgtggcga ccgtctcgcg cgaccaccag cggaaagtct ccgcgctctt ccccgccgcg
    24781 gacgtccggt acgtcccgaa cggcatcgac ccggcggagt gggtgctcgc gccgcacgac
    24841 ctggagcgcg ccgcgcggtg gcgggccgag cacgtcggag agggacggcg ggtcctcggg
    24901 atgttcgggc acctgaaacg gaagaagggg ggcgccttcc tcctcgacgc cctccggcgc
    24961 gcgggagtcg agggaaggct ccacctcctc gtcgtcggcg aggccgagcc ggaaatgctc
    25021 gcgcatctcg cgtcgatcga gtcttcggtc ccgttcacgc acgtcccgtt cgtcgaccgc
    25081 ttcgagctcc tcccgtacta cgccgcctgc gacggcgtcg tcgtcccttc gctctacgac
    25141 ggccttccga acgtcctcct cgaggccggg gcgctcggcg ttcccgccgt cgcttcgacg
    25201 gcgggcggca tggccgacct cctcgagggc gggggaagcg ggctcacgtt cccgcccggc
    25261 gacctccacg agtgcgggcg cgcgctcgcc acgttcgccg cgctcccggc cacggagctg
    25321 cgcacgatgg gggaacggct tcgagccttg gtcctccgcg atttccgcca cgaccaggaa
    25381 gcggcgcgct accgcgcgct cctcgccgaa accgccggac gcacgtcgcg cccggttccc
    25441 gccgacgtcc tcacggagag ctgagccatg aagaaccctc tcgcccgcct cctcccgttc
    25501 gccctcctcg ccgtcccgct cgcggccggc gccgtgaccc ccgcggacgt gcacaccgtc
    25561 gtcgtccgct cggccggagg ctcgaagagc ttcacgatcg tcgagaacgg gaacgcgatc
    25621 gaaatcgtcc tcccgggcgg agtccgactc ctcggcgagc cggccgggga gaagcggaag
    25681 taccgccgcg gcgcgggcgg tccgccggtc tgcgaggtca agccgggcga cgccggcttc
    25741 aagctccgga cgacggacgg gaagctcctc tggaaggtga agctcgccga cgacaagatc
    25801 aaggtgagcg acaacgagga gaacgcgaac ccgtactcga tcaagttgaa gtaccccgac
    25861 aaggcgaagg tcgtcgacgc cgccgagaag gagcttgccg aggtccgctt cggccccgac
    25921 aagacgaagg tgaagggggc cgacggcgcg gagctctggg agtgccgctc gccgcgccgc
    25981 aggacggcgt cgttcggcgt cctcgtcctc gagaggatcc cggaggagca ccgggcgatc
    26041 ctgatggcgg agatcctcgc gcgccgccgc tgaggcgcgc gtgctcgtct actacgcgct
    26101 cgggggcggg ctcgggcacc tcgtgagggc ccgggccttc ctccacacgc tcgggctcga
    26161 gacggaggcg accctcttca cctcctctcg cttcgccgac gaccaccgtg tcactggcgg
    26221 cctccccgtc gtccgcgtcc ccgattcgct caacggcgac cgcgccggcc tgcgcgctct
    26281 cctcgaagag aagatcgccg acaccggcgc gacacgcctc gtcgtcgacg ccttccccgc
    26341 cggcctcctc ggcgagctcg cggagtgggc tccgcccgcc ggcctggcgc tctggcacgt
    26401 cgcgcgcctc ctccggtggg accgctacac cgccgatgcc tccccccgtc tcccgcgctt
    26461 cgacgtgacc ttcgtcgtcg aggagcttca cgtcgagcac cgccgggcgc tcgaagcggc
    26521 cacccgcgag cttcgcccgc tcgacctcgt cgacccgccg gctcccccgt ccgagccgat
    26581 cgaggggccc cacgtcctcg tcgtccactc cgggcccgac gaggagacgc tcgacctcgt
    26641 cgcctgcgcc cgcgagctcg ccgctgcgga cggccgcggg gctcgcgtcg tcctcgcctc
    26701 gcccgcccgg cccggttccc tccccgcaga cgtcgagtgg cgcgacgcct tccccgcgac
    26761 cggcctcttc cccgccgccg accggatcgt cgccgcggcc ggcttcaacg ccgtgcggca
    26821 aacggagccg ttccgcgcga agcgcttcct cgtcccctac ccccgccgct tcgacgaccc
    26881 gttcgcccgc gccgcccggg tccggtgccc ccaggctcgg tgccaggcgt gaattcgtgt
    26941 attgttgaaa acaatacacg aattcacgcc tggcaccaca cggggtggca ccacacgggg
    27001 agcgccccac ccttcccgcg ccctgcgttc ccgcgacgtt tcgtccggcg gggcttgacc
    27061 gtctaatatc cgcccgctcg aagctcgaac gaagaagggg gtcctcgatg cgggacgctg
    27121 ctgtgcggtg cgctcacgcg ccgcgaacga cgtttctcgt cctcctcgtc gcgtacgcca
    27181 cgtccctcca tggcgcgccg cccgccgacc gccctgcggg gggcgcgacc gccggcgccg
    27241 tgcccgcggc tccggccgtg agcgggttcg cgcgggtcga cgacacggag ctccggctcg
    27301 cccacggcta cctcctgcgg gccccggacg cgttcaaggc gtcgcagcag aacgcgatcg
    27361 tcctcctgac gccgcagccg ctcgacccga agaagctcga cgccgccggc actctcctcg
    27421 cggcgctcga cctctcgccg cagcgcgtcg tgctcgaggt cgagcccgac agatcggtga
    27481 ggctctggat ctgccacgac ggcttcgggg ccggcaagtg cttcatcacc ccgattgcgc
    27541 cgttcgactg gaagcccggt gtcgtcgaag agaagcacgt ctcgggaagt ctgaagagct
    27601 tcgccgggaa ggaggagacg gtgctcgaga cgttccggct gtactacgag gtccagtttg
    27661 acgtgaaggg cggccgggcg ctcgcgaccc gccgctgagc gggacagcgc ggcgccgctc
    27721 tcgtccgacg agacggtcgc cccgggcgag gccggcgctc gtacgccgct cttcgagggg
    27781 cccgggaccg tgctcgaccc ctaccggctg atcgagagga tcggtcaggg ggcttcggga
    27841 cggtcttcct cgccgagcag caggagccga tcgtccggcg cgtcgcggtg aagctcctga
    27901 agcccgggat ggacacggag agggtcgtcg cgcgcttcga ggccgagcgg caggcgctcg
    27961 cgatgatgga ccaccccaac atcgctcgcg tcgtcgacgc gggcgcgacg ccgtcgggac
    28021 gccccttctt cgtcatggac ctcgtcgtgg gggcgccgat ctcgacctac tgcgacgaga
    28081 acgcgctccc cgtcgcggcg cgcctcgggc tcttcgccca ggtgtgcaac gccgttccgc
    28141 acgctcacac gaaggggatc atccaccgcg acatcaagcc gtcgaacgtc ctcgtctcga
    28201 cccacgacgg gaagccccac gcgaaggtga tcgacttcgg catcgcaaag gcgacggcgg
    28261 tccggccgga gggcgagacg ctcttcacgg tggacggggc gatgatcggc acgcccgagt
    28321 gcatgagccc ggagcaggcc gagcgctcgc tcgacgtcga cgcccgcacc gacgtctact
    28381 ccctcggcgt cctcctctac gagctcctca ccgggtcgac gcccttcacc gcgcgcgagc
    28441 tgaagtccgc ggggctcgcc gagatccggc ggatcatcca cgaagtcgac cctccgaagc
    28501 cctcgacccg catcggacac tccgccgaca cggcggtcgc caccgccgcg agacggcaga
    28561 cggctccgaa gcgcctctcg gcgatcgtcc gcggcgagct cgactggatc gtcatgcgcg
    28621 cgctcgagaa ggaccggcag cgccgctacc cgacggcgaa cgccctggcg atggacgtcc
    28681 agcgtttcct gagcggcgag ccggtcgtcg ccgcgcctcc gggcgccgcg tatcgcgccg
    28741 ggaggttcat gaggcgccac cgggccgccg tcgtcggagc cgcggccgtc gccgtcgctc
    28801 tcgtcgccgg cgccgtcgcc gtcgcctggc aggcgacgat cgcgcgcggc cagcgcgacc
    28861 gcgccgtgtc cgccgaggcg cgggcccgca ggacggcgga cgagttcgca aggtctccga
    28921 cttccaggcg gcggtgctcg cgcaggtcga cccggcgtcc gccggccgtt cgctcgagaa
    28981 ggaggtcgtc gggcagttcg agaggacgct ggccgaggcc gacctttcgg agcgggagcg
    29041 ggcggcgcag cgggcggcgt tctccggcca gtgggggcgg gtcaacgcca ccgacgcggc
    29101 gcggattggc acggacggcc ctcgggaagc tcgacgccgc ggagaaggac ctcctcgagg
    29161 cctaccctct cctcgtgtcg acccgtggag aggagcacaa ggagacgcgc gagtgcgcga
    29221 aagcgctcgt cgagctcttc cgggcccggc aggcttcgaa gccggggcgt ggccacgatc
    29281 gaacggcctc ttcctgggcc gcgaagctcc gcccaacggc ccgcacccgg taggctcccg
    29341 caccctcggt ctcgatcgac cggtgcctcg ggtggtcctt gccgccgggc gctacgatgc
    29401 cgtcgtgtcc gccgcgctga cgccgccgcc cgcgtggggg cccgggcctc tcccgccgcc
    29461 ggctccgggc gggaccgtct cggtgcgccc cctcccgaac ggcgaagaga tcgtccttcg
    29521 ggcgacgggc tggctgcgcc tcctgccggc cgcgttcctt ctcttctggc tctgcggctg
    29581 ggccgtgggc gagctcctcg tcctcctcat cctcctcggg ccgctcgccg acccgctcgt
    29641 ctccgcgctt cgcgaggtgc tcccgggagc ctggccgcgg ccgccccggt tcgcgggctc
    29701 gacaccgtgg ccggccgtgg ccttcctcgc ggtctggttc gtcttctgga cctggggcgg
    29761 gctgacggcg gcctggcagc tccttcggct cgtcgcgggg agcgaccggt tcgtcacccg
    29821 cccgggcggt ttctctctga ggcccggcgc aggccctttc ggccggacga gagacttccc
    29881 cgcctcggag gtctcggccg tttacgccga cgtccgccgc tcgcgcctcg tcgcggtcgt
    29941 ccggggctcg gagaggctcc tctgctccgg gaccccgccg cccgtggcgg agtggctcgc
    30001 cgcccgcctc cgggccgccc tcggcctccc cgggccgacg ctcgccgatt cgggagagcg
    30061 ggacgccgag gcctccgccg agcctccggt cccggtggac tgggaggccc ggccgctccc
    30121 cgagggaggc gtcctcctct cggcgccccc cgcgaggtcg cgaaagacgg ccggctgcgc
    30181 gctcgccgtc gcggtcgtcg ttacggccgg attcgtcgcc gtgctcctcg ccgccggcgt
    30241 tcgccgcgag tcccccggag ccgccctcgt cttcgggctc ctgatcctcg ccgtcgacgt
    30301 cctctgcagc tggctcgcgt tcgcgcgcga gtcgtggctc cttcgtcccg gcctcatcgc
    30361 gagggtgcgg agcctcggcc cctgggagcg ccggcgcgaa gtgcgtcacg gctcgctcgc
    30421 ggtgtccgtg tcgaccgatt cggacgacga cgactggttc cgcctggagg ctcgcggcga
    30481 gggccggccg ttgaagctgt cgagccggat caacgccggg ccggaggtcc tcgccttcgc
    30541 gcgctacgcg gcccggcatt ccggattccc cctggacgtg ccgcacgagg tgcttctcgc
    30601 ggccggcgcg ccgcggaacg gaagctcgag agcgcggggt ccggcgtgag cccgtccccc
    30661 cctgccggcc gcggctacgg cttcgccggc gggtcgagga gaccgttcct ccgcgcgggc
    30721 gtcccggcgg cgaggccgtc gaggagggcg tcgaccgtcg agacgccccg ggcggcgaga
    30781 gcgcgggcga gaccgtcccc ttccacgagc cggatgcggc gctccagctc cggccggaat
    30841 tccggcgtga cgtagaaacc cgtcgtgccc gagaggcgca gctcgtcgat cggcccgagg
    30901 aagatcagcg cgtcgtaggc atccgccatc gtcgatccgg gagccgcgtc gagcatcgtg
    30961 ttcccgacga accgcgcggc gccgaacggc gtgcccgcga gctcgaggcc gacggggacg
    31021 ttgccgttgg ccgcgaaggc ccggtcccag attccgtcct ccatccgcac ccagcggtac
    31081 gacttcgact ccagcccctg ggtcgaacgg gggccggcgg cgtgcacgac cccttccacc
    31141 gccagcgtcg cgttgtggag gcgcgccgac caggcgcgcc ccgggtcgtc gcgcccgaag
    31201 aagccggcgg cgttgagctg cggacgcccc cggccatcgc gcaggcgcat gtacgcgtgg
    31261 cgcgtgttcg tgaggaagaa cgccttctcc ccctcgcgaa agccgtcgag gcggctgcgg
    31321 atcgtcgcgt agaggaagaa gtcgcggccc gcgagagagt cctggaagag ctcgtagtcg
    31381 gcacgcgtcc ggatggcgtc ccaccagatc ggctggtcga cgccgacgag gcgcacgcgc
    31441 tcggacggcg gaagcccctg gttcagctgc cgcacggtcg ccaggagctc gaggtaggtg
    31501 gagtaccgcc agccgtagcc cgagaagtcg tcgcggaagg ccggagcgag gaggctccgg
    31561 tcgtccgtcg ggctcgcgag gtaggcgtcg aggtgcggct gcgccgcgat cgagaagacc
    31621 tccaggaaga ccgttcgcac gcgggcgcgc acccggcggt cgcgcagcag cgacgcgtag
    31681 agctcgaacg gctcgacggc attgtgcagg gcgtcgtcga aaatcagcag gtcgtgcttc
    31741 tcgagggcgc cgaggacgag gtcgactggc gtccgccccc gctcctccag tgcccgcacg
    31801 tacgcttccg gcgcgctccg ccccgtcgtt cccgtcaccc cgagcgccga cgccgcgagg
    31861 acgcaggcga cggcggcgct cacgacgggg aggagcgggc cacgacccgc cgacctcgca
    31921 cgtctcggga agggccccgc ggacctcggc tgagtcgacg ccatggttct cctccttccc
    31981 cggccatcgc ggccctgccc gttgctcgct cgtgtcgacg aggccccgct ccagaggatc
    32041 gcggctctcg cggctcagac gccgggtggg atacggctgg acctcaccgc cggttacgcc
    32101 ctcaggggcc gctcatgccc gagcttcagg cctcagccga gtggcgtcgg cgccggctca
    32161 gtggcagtgc gtcgtcgagg cgcggccatg gacggtcgtt ccgtccgcca gcgcgcagtc
    32221 gagctcgagg gagagtcggt agtcgcggac ctcgttgatc gtcaggcccg tgtgctcgag
    32281 cgagaagtgg aagacggcgc actcggagcg tcgcaggacg acgctctgcc ggaacgggtc
    32341 ggcggcgtcg aagacgcgcg cggcaggtcc ctcgagcggg tcgacgacga ggcggacgac
    32401 gagacccgag gcggggtcgg agaaatcccc tccgaggaac tgctgaaggt ccccgctgac
    32461 gcagcccgtg ggcgcgacgg tcgtgttccc gaggagaggc gagctcaccg cgaaggaccc
    32521 gagagctccc gcgggagccg tctccgtcgc ggcgtaaccc gggagcggca tcgcggggaa
    32581 cgagaagcag ccggtcgaca ggacgaccgc cgcacaaagg aggctcgcga gaccgttcgc
    32641 ccgagcgtgc cgccgtttca tggcggacga gtctttcacg gacgtcggcg ccggttccgt
    32701 gatgctcctc gcgctccggt cgttccccgc ctcccctgcc cgaggtgcgg gtcgcgtcgc
    32761 gccgtcgcag gacggacgcc tcccgactcc tcctccggtt gatccagcgg aaggggatgc
    32821 ctcgagcggg ccgtgtcgtc cgtgccgctc cgccacgcgc cgccgaacac ggactcggtg
    32881 ccaggcggac tcggtgccac acggactcgg tgccaggcgt gaaatcgtgt attgttatga
    32941 acaatacacg atttcacgcc tggcaccata tcgaggtggc accatttcga gggcaccatt
    33001 gcgaggcacc gtgtcgagat gccgaaaggc gtcccggaac gcgtcacgac atctccccgg
    33061 ccggcgaggg cacgggcgcg ggcgcgcggc tcgcccgcga gacggcgagg gcgacgacga
    33121 ccaggaccgc ggccaccacg aacgtggccc gcatccccgc ggcgagggca gcggggtcgg
    33181 cggcggcgac gtcggccgca cccgacgccc tggcgaagac cgagcccatc acggaggccc
    33241 ccgtgatgag gccgaggttg cgcgacaggt tgaggagccc cgagacgacg ccccgctcgc
    33301 ccgcgtcgac gtccgcgagg acagccgtgt tgttggccgt ctggaagagc gcgtagtggg
    33361 ccgtgatgac gacgatcggg atgacgtacc cgacgactcc ggccgccgcc ggcgtcatcg
    33421 acaggccgac cgcgccggcc acgatcccga ggagccccgc cagcgtcgtc cgcggcgcgc
    33481 cgaagcgatc gaccgcgcga cccgccggca cgccggtcat cgccgcgacc agcggtcccg
    33541 ccgacaggac gagcccgacg ctcccggcat cgagcccgag cgcccgagcg aggtagaacg
    33601 ggccgaccac catcgtcgcc atcatcaccg tcgacacgag ggcgctcatc gcgaagcccg
    33661 cgccgagcgc cgggtcctgg aacgccgcca cccggacgag cggcgccttc gcccgcgcct
    33721 cgacccgtac gaagagcgcg acgccgagcg ccgctcccgt caggagcgcg gcgccgcgcg
    33781 ctccgagccc gccgcgcccc accgtcatcg cgagcgcgta cgacgcgagg gtcagggcga
    33841 ggacgaccgt gccggcgagg tcgaagcggg gccgaccggc cttcgccgct cgacgatcgg
    33901 ccggaaggta ccgccgcgcc atcaccagag cggcggcgcc gagcgggagg ttgaccagga
    33961 agatcgcgcg ccatccgagc ccggcgatca ggagcccgcc gagcgagggc ccgagcgccg
    34021 tgccgaccgc cgacatcgtt ccgaggagcc ccatcgcggc tccggtccgc tccctcggga
    34081 ccgtctcgcc gacgaacgcc agcgtcagcg ccagcatggt cgccgcgccg acgccctgca
    34141 gagctcgcgc cgcgacgagc atcccgagcg tgggcgcgag gccgcacagg gccgaggcga
    34201 gcgtgaacac gaggatcccc gcgctcagca gccgcctgcg tccggtgacg tctcccagcc
    34261 gtccggcgcc cacgacgagc gtcgtcgtgg cgacgaggta ggcgaggacg acccactgca
    34321 ccgcgtggaa gggcgcgcgg aacgaggtcg ccagcgccgg cagcgcgacg ttggcgatgc
    34381 tcgtcccgag agacgacagc agcatcggca gcgcgaggct cgcgagcgcc catccgaccg
    34441 gagtccgtcg ctccgcctct cccatcgccg cctcgtcccg tctgtcgcgg atcgcattca
    34501 tcgtgagccc tctccttgcc cggcttcgga ttcggtgccg cgatcaaggt aggcgcgatc
    34561 acggcatggc ggaagacgca cacattgcat tgaatacatg catgaaacgc catgttcctg
    34621 tcggttcgtg ctacgttcgg cccgtggcgg ccccggacct caacctcctc gtcacgctcg
    34681 ccgtgctgct cgccgagggg agcgtcgcgc gcgcttcccg gaggctcggg ctcagcgcct
    34741 cggcgatgag ccgagcgctc gcgcggctgc gtgcgacgac cggcgatccg ctcctcgtcc
    34801 gggcgggccg gggtctcgtc ccgaccccga gggcgatcgc ccttcgcgac cgggtcggtc
    34861 cgctcgtgca ggaggcggag gcgatgctgc gcccggccga gaacatccgc ctcgatacgc
    34921 tggagcggag cttcacgctg aggtcggccg agggcttcgt ggagagcttc ggcgcgagcc
    34981 tcctcgcgcg agtcgcccgg gaggcccccg gcgtgaggct gcgcttcgtg cagaagctcg
    35041 acagggacaa cgtgcccctc cgcgacgcga acgtcgatct cgaaacgggg gtcgtcgacg
    35101 acacgaccga cccggaggtt cgcgtccagg cgctgttccg cgaccggttc gtcggcgtcg
    35161 tccggacggg gcacccgctg tgccggggga agatcactcc ggcgcgatat gcggcgggcc
    35221 ggcacgtctt cgtctcgcga cggggccgga gcacgggacc gatggacgac gccttgaagc
    35281 ggctcaggct ggagcgcgag atcgacacgg tggtcggcgg cttctcgacc gcgctggcgc
    35341 tggcgcgcgc gaccgacctg atcgccacgg tccccgagcg gcacaccggg agcctgcgcg
    35401 acggcatgca cagcttcccg ctcccggtcg ccacaccggg gttcaccgtc tcgatgctct
    35461 ggcacccgcg gttccatgcc gacgccgcgc accgctggtt tcgcggctgc gtcaaggagg
    35521 tgtgtgagga gccgccgggg cgggcgacgc tcgcagcccg gccgccggcg cgcagcgtca
    35581 ggcgtgtggg gtcaaggcga ggaaccgtgc actccctctg aaagtacgcg gtttcacggc
    35641 tgaccccgcc tttgggcccc cgttgtcggg ccgagcccgg ctgccctacc ccaggtacgg
    35701 gtcgcgccac tccgtggccg ggacgaaggc ctcctcgatc gttctcgggc tgatccagcg
    35761 gaggaggatg gctcgagccg gccttgtcgt tcgtgccgct catccgcgcg ccgccgaacg
    35821 gctgctggcc gacgaccgcg ccggccagcg ttcgcctggc aagaagagct tggatctctc
    35881 gctggcacgc gcctcgcttg gcaggtgcag gcgtactcca ttgggtacgc gggggcctcg
    35941 tgatcgcatt cgcgtctctg tgggtcgggt tcgtgatcgg aatcgtcaac gtgcagctga
    36001 tcgcctcgga aggtgtgagc cgggtcgagc tcatcctcga cggccgacct gtcgccgagc
    36061 tccgtgcccc cttcgcggcg ccgctcgatc tggggtgcga cccctctccg cacgagctcg
    36121 tggcggtcgc cttcgacgcg aaagggaagg agctcgacag ggcgcggcag tggctcaacc
    36181 gcccgcgccc gacggcggaa gcgagcctcg tcctcgaagg gggacacggg ggaagcggcc
    36241 gggtcgcgcg gctcacatgg cgcgctctcg cgcgggaggc gccgacctcg gtcgcggcga
    36301 cgttcgatgg cacgccgatc ccggtgagcg accccgctcg catcgagctg ccgccccacg
    36361 agccggccca gatccacttc ctgaagacgg tcctggaatt cgggacaggc gtctccgcaa
    36421 ccgcggagct gatcttcggc ggctcgaaga cggtcgagga atacacggag ctgacggcga
    36481 tggctgtcga gctggagcgg aaggcgtcgc tccctcccgt cgagacgatg gccggatggt
    36541 tcgaggagaa cggggttccg ctcgaggtcg cggccgtgga cgccgggggt gtcgacatcg
    36601 tcttcgtcgc ggaagggagc gcccggaagg agctcaagca ggcgttccgg cgggaatccc
    36661 tcaacgatgc agagactgcc gccaatctcc ggaacggcgc acgatttctg cgggaggacc
    36721 tccgctaccg cttcctctgg cctgtgggac ggctgaccgc gcagacggag atggtcgcga
    36781 atgtctacca atcgacgccg tggctctcgc ggaagagaag cagcatcggc cgcgctgcgg
    36841 gctggctcct cgactggccc ggctggagcc gggacgggca acggctcgcg gacgccgtcg
    36901 ccgtggcggg cctcacggcc gccgaggcga accggaggcg ggcggtcgtc ctcctcctcg
    36961 gtcccgcggc gaaggacggg agcctgctga ctcccgatga ggcgatgagc ttcctccggc
    37021 gtctgggcgt cccgctcgcg gtctggtcga tcggaaagcg ctcacccgag acgtcgcggt
    37081 ggaatgccag gaccctgatt tccgcgtacg agcaccggga gttcgacgac gccatgcgcg
    37141 ccctcctgga aagtgtcgac cgccagagga tcgtctggct cgagggcgcg cacatgcctc
    37201 acgaggtcga gacctcgcgc ttcgcgcacg ggattcggcg ggtgcaatga gcgtggctcg
    37261 ggatgaacac cgtgcgtgcg ggcgccgacg gctcgcgcgc cgcaatcgaa cggaggggac
    37321 cgcgtgatct cgttcgccac tctctgcttt ggtgtcgtct acggggtcct caacgtcgag
    37381 attcgagcgt cggacgatgt cgccagggtc gacctcgtgc tcgacggcag gccggtcgcc
    37441 gagctgaggg ctccgttcgc cgcgcccgtc gacctcggtt ggcaacccgc gcctcaccag
    37501 ctcgtcgcca tcggatacga cgagaggggc aaggagctgg gccgcgcgaa gcagtggatc
    37561 aacaggccac gggggaacgc ggaggtccat ctgctcgtgg agcccgaggg cacggagcga
    37621 ggtcgcctcg cccgcctgac gtggagctgc ccgacatcgg gcgcggccgg gcccgtcgag
    37681 gtgaccctcg acggccggaa gctcccggtt tcggacccct cccgaatcga gattcccgtc
    37741 agggacgcga gccgggcgca aattctccgc gcccgcgtgg agtgtggcgg gaacgtcacc
    37801 gccatgaccg agaccgtcat cggcggggat cgtccgtcga ccggatcgcc acaggtgacg
    37861 gccgttcccg tccacctcga gaaggggacg aagctcccgt cgccggaggg gctcacgggc
    37921 tggctcgaga gggatggagt cggcctccgg gtcgccgccg tcgaggaggg agggttcgac
    37981 gtcgtcttcg tctgcgaggg gggcgcgttc gaggagctga cgcgactctt cgaaaggcgc
    38041 ggcgccccgg ttcgcgactc ctccgaagac gacctccggt ttcgattcct cgtacctgtc
    38101 ggccggacgg tcgacgatcc gacggtcgat tcgagctcct atgcggcgac cgcctggttc
    38161 cagcgaaggt gggacgccat tggcccgacg ctcacgaacg gtctccagct caagccctgg
    38221 ccacggacgg agcagcgaat cgcggacgcg gttgccgcgg ccgggctcgc ggccgccgca
    38281 cgggagcgcc ggagggccgt cgtcctgctt ctcgggccgg gcgcccggga cggaggcgat
    38341 ctgaactccg gcgaggcaag gcaattcctc atggatcttc gcgttcccat cttcgtctgg
    38401 tcgatcggca aggcgccatc gccggagacg gggacgtggg atgcgacgtc gacgatcacg
    38461 accgcggcac agttcgacgt cgccgtcacc cgccttcgcg cggagctccg gcggcaacgg
    38521 atcctctggg tcgagggagc ccacctgccg caggtcatca cgctgacgcc tcgcgcgaag
    38581 ggcgtccgtc tcgcgcgctg agcgccctcg ctgggcacat cccgaagatg ccgatcgaga
    38641 cagtgatcgt ctcggaatcc gccccgctct ggcgcgagga gacgagactt caggggccgg
    38701 ccaagagcag gtgtcaggcg tgaaatcgtg tattgtcggt gacaatacac gatttcacgc
    38761 ctgacaccac gacccgtgac accacgaccc gcgtttcggc gcccgcgagg ccaggtccgg
    38821 gcgacgtcgg cagggaagac gagagtcacc gacggaacgg gcggcgcggc gcctcctgct
    38881 gctaccccag gtacgggtag cgccactccg tcgccgggac gaaggtctcc ttgatcgtcc
    38941 gcgggctgat ccagcggagg aggttcaggc tcgagcccgc cttgtcgttc gtgccgctca
    39001 tccgcgcgcc gccgaacggc tgctggccga cgaccgcgcc ggtcggcttg tcgttgatgt
    39061 agaagttgcc ggccgcgtga cgcaggtggt ggaccatgac gtggagggcg tagcggtcct
    39121 ggctgaagac ggcgccggtg agggcgtacg ggctcgtcgc gtcgacgacg tcgagcatct
    39181 cctcccactt cgcctcgtcg tagacgtgga tcgtgaggat cggcccgaag agctcgtggc
    39241 acatcgtgtg gtacgagggg tcgagcgcct ggatgatcgt ggggcggacg aaccagccga
    39301 cggaggagtc gtacgtcccg ccggcgacga tcttcgcgtc cttcgacgcc ttcgccgcct
    39361 cgatcgcttc cctgtggtcc ttgaacgcgg cctcgtcgat caccgcgccc atgaagttcg
    39421 tgaagtcgcg cgggtcgccc accttcagcc cctcgacctc ggcgacgagc tcgtcctgga
    39481 tcttcgccca gaggttcgag gggacgtagg cgcgggaggc cgcgctgcac ttctgtccct
    39541 ggtactcgaa ggcgccgcgg aggatcgccg tcgtgagcgc cttcgggtcg gcgctcgggt
    39601 gggcgaagat gaagtccttg ccgcccgtct cgccgacgag gcgcgggtag gagcggtact
    39661 tctcgaggtt ctgccccacc gtccgccaca tcccgttgaa gacgcccgtc gacccggtga
    39721 agtggatgcc ggcgaggtcc gggtggttga ggacgacgtc gccgatggcg cggccgctgc
    39781 cggggatcat gttgatgacg ccctcgggca tccccgcgtc gcggtagagg cgggtgagcc
    39841 agtacgcgct gtagagcgcg gtcgtcgccg gcttccagag gaccgtgttc ccgcaaaggg
    39901 cgggcgccgc cgcgaggttg ctcgcgatgg ccgtgaagtt gaacggcgag acggagaaga
    39961 cgaacccctc gagcggccgg tgctccatcc ggttccacat gccggggccg ctctcgggct
    40021 gctcggtcag gatctcggcg tagtagtgcg cgttgaaccg gaagaagtcg atcgtctcgg
    40081 cggcgctgtc gatctcggcc tggtggcacg tcttgctctg accgaggacc gtcgcggcgt
    40141 tcaggacgtc gcgatacggc ccggcgagga ggtcggccgc cttcaggaag atggcgaccc
    40201 gctcctccca cggcatccgc gaccagaagt ccttcgcgcg gtcggcggcc gcgacggcga
    40261 gcgcgacttc ctcggggccg gcgaggtgga cgtcggccag gacgtggccg tggtcgtgcg
    40321 gcatcaccgc cttcgcgagc ttccccgtcc ggatctcctt cccgccgatg acgaggggga
    40381 tctcgatccg ctccgaggcc atctcggcga gcttcttctg gaggctcgcg cgctcggggc
    40441 tgccgggcgc gtagtccttc accggctcgt tcttcgggac cgggactctg tagatgccga
    40501 gcgacatggg gcctccttgc cggtggcggc gcggtacggg gtcagatctc gagcgtgatc
    40561 ccctgggcga gctcgaggcg gccggagaag ttgagggcgt tcgtctggcg gcgcatgtag
    40621 gccttccagg cgtccgagcc gctctcgcgc ccgccgcccg tctccttctc gccgccgaag
    40681 gcgccgccga tctcggcgcc gctcgtcccg gtgttgacgt tcgcgatgcc gcagtcgctc
    40741 ccctcggccg agaggaagag ctccgtctcg cggtagtcgt tcgtgatgat cgcgctcgag
    40801 agcccctgcg ggaccgagtt ctgcatcgcg atcgcttcct cgatcgtccc gtacttcatg
    40861 aggtagagga tcggcgcgaa ggtctcctcc ttcacgatcg ggaggtcgct cgacgcctcg
    40921 acgaggcacg gcgtgacgta gcagccgccc tccttctcga gcggctcgcc gccccggagc
    40981 accgtcgccc cctgcccctt cgccgtctcg atcgccttcc gcatcgtggc gacggcgtcc
    41041 cggtcgacga gcgggcccat gagcgtgtcg taggcgagcg ggtcgccgat cgtcgtctcg
    41101 gcgtagaggg ccgacaggcg ctccacgaac ctctcgtaga ccgactcgtg gaggatgacg
    41161 cgccgggtcg acgtgcagcg ctggcccgac gtgccgatcg ccccgaagta gaccgcgcgg
    41221 atcgcgatgt cgaggtcggc cttctcgctc acgatgacgg cgttgttccc gccgagctcg
    41281 aggatgagcc gcccgaagcg ctcctggacg agcttgccga cgtggcggcc gcccgggacg
    41341 gagccggtgt aggagacgag cgcgacgcgc gggtcggtgt tgatgaggtc gccgatgtcg
    41401 ctcccgcggc cgatcgtgag cgagcagatc gcggggtcgt agccgaagcg ctcgagggcg
    41461 cgccgggcga tcttcgtgac ggcgatcgcc gtgaggggcg tcttgctgga gggcttccag
    41521 aggaccgtgt cgcccgcgac gagcgcgagc gcggcgttcc agctccagac ggcgacgggg
    41581 aagttgaacg ccgtgatgac gccgacgacg ccgagcgggt gccactgctc ctggagccgg
    41641 tgctgccgcc gctcgctcgg catcgtgagg ccgtagagct ggcgggaaag gccgaccgcg
    41701 aagtcgcaga tgtcgatcat ctcctgcact tcgccgagcg actcgcggag ggtcttcccc
    41761 atctcgaggg agacgaggcg cgcgagaggc tccttctggg cgcggagctc ctcgccgatc
    41821 gccttgacga cctcgccgcg cttcggcgcg gggacggtgc gccacgacgc gaaggccgcg
    41881 tgggtcttcg cgaggatccc gtcgaactcc tccttcgtca cgttgacgac ggaggcgagg
    41941 ggcgagccgt cgaccggcga gacgacctgc tggaccttgc cgctgccgac ccacgcgccc
    42001 gagaagcccc ccgggttcct ctcctcgatc ccgagcgcgc gaaggatctc cacgtccgtc
    42061 tccgtcttca gcgacatcgt cctacctccg ggggaggtta tgcgacagaa tttcaaagaa
    42121 atgaagctca ttgtcttcat ctctcaaatc gatatcattc atgcatgtca acgctcgacc
    42181 tctcgcagct ccgcgccttc cacgccgtcg ccagggcggg cggcttctcg gccgcggccg
    42241 cgcgcctcaa cctgacgcag agcgccgtca gccacgccgt cggaaagctc gagcgctcgg
    42301 cgaaggtgcg gctcttcgac cggcgcgggc ggaaggtcgt gccgacgccc gaaggagcga
    42361 tcctgcgcga ggcgtgcgag cgggtcttcg cgacgctcga agcggccgag gaggagctcc
    42421 agcgcgagcg gagccgcgcc gaggggcgaa tccgcctcgg cgcgacggtg gagttcggga
    42481 cgagcgtcct gatgcgccac atccgcccct tcctcgaggc gaaccccggg atcgagatgg
    42541 acttccacct ctcgcacgac ctcctgacgc cgctcctgcg cgacgacgtc gacctcgcga
    42601 tcgactgcgt cgcgcacgcg acgcccgagc tcgagaggac gccgctcttc cgggagacgt
    42661 acatggtcgc ctgcgcgccc gactacctcg cggcgcagcg gatcgccgag ccggcggacc
    42721 tcgcccgctg cgccgtcctc tcgatcgaca aggaggggcg ctggtgggag cgcttcctcg
    42781 gcgcgttccc gaagagccgc cgcccgtcct tcggccgcgt cgtcgccatc aaccacatcc
    42841 gcggcctcat caccgcggcc gcgagcggcc tcggcgtcct cctcgcaccg agctacagcg
    42901 tgaagggaga gctcgaccgc ggcgccctcg tcccgctctt ccccgcgttc cgtcccctcg
    42961 aggaccgctt cgccctctac cagaaggcgg gcaaggcgcg cctcgccaag caccgcctcc
    43021 tgacggagta cctcgagcgg ctgacgccgg aggagttcgg ggcgtagggg ccggccgccg
    43081 gcctcgccgc gtgtcgctcc ggccgcgacg cgccggctcg aaccggaccg gacgcgcggc
    43141 tctccccccg gcctctcgtt gcgatacgct ctcagaaaga atttccgtct ggagggcgaa
    43201 tcgatggcaa ggctgaaggt cgcttccttc tcgatctcgc tggacgggtt cggggccggg
    43261 cccgaccagt gcctcgagaa cccgctcggg ttcgggggcg ctcagctcca cgcctgggcc
    43321 tttccgaccc ggacgctccg gcggaaggtc ttcgggcacg acgacgggga gacgggcctc
    43381 gacgacgact tcgtcgcgcg agggttcgag gacgtcggcg cctggatcct cggccggaac
    43441 atgttcggcc cggtccgcgg accgtggccc gacgagagct ggaaaggatg gtggggcgag
    43501 gagccgccct accacgtccc ggtcttcgtc ctgacgcatc acgcgcgtcc ctcgctcgag
    43561 atggaaggcg ggacgacgtt ccacttcgtg acgggcggca tccacgaggc gctcgagcgg
    43621 gcgcgcgagg ccgcgggaga gcgggacgtc cgcctcggcg gcggcccggc gacgatccgg
    43681 cagtacctgg cggaagggct cgtcgacgag ctccacctcg ccatcgcccc cgtcctcctc
    43741 ggctccgggg agccgctcct cgcgggcctc gatctccggg cgctcgggta cgcctgcacc
    43801 cgccacgtcg cgtccgagcg cgcgacgcac gtcgtcctgg agcggacgcg gcacacctga
    43861 gccgcgcgtc cgccacggaa aggagacccg ccatgccgaa cacgatccgc ctccaccgcg
    43921 tcctgaaggc cccgcccgac cgggtctatc gcgcgttcct cgaccccgac gccatggtga
    43981 agtggctccc gccgaacggc ttcaccggta aggtccactc gatggacgcg cgcgtcggcg
    44041 gctcccaccg gatgtccttc acgagcttca cgaccgggtc gagccactcc ttcgggggca
    44101 cctacctcga gctcgtccct ggcgagcggc ttcgctacac cgacgccttc gacgacccga
    44161 acctcccggg cggaatggaa gtgaccgtga ccctgaagaa ggtctcctgc ggaacggagc
    44221 ttcagatcac acaggaaggg atccccgacg tcatcccgcc cgaagcctgc tacctgggct
    44281 ggcaggagtc gctcgtcctt ctcggcaagc tcgtcgaagt cgcggagtga ccgccgccca
    44341 ccggaccgct cgacgacgcc gccgctaaga tcgcccggtg gactacccgc ggatcctcgc
    44401 cgagatcgca gaagaggtgc ggccgctcgt cggacagggg cgggccgcgc agtacatccc
    44461 tcccctcgcc gaagtcccgc gcgaccggtt cgggatggtc ctccgaacgg tcgggaacga
    44521 ggcctactcc gtgggtcacg cccgggagcg gttctcgatc cagagcatcg cgaaggtctt
    44581 cagcctgacg ctcgcgacga ggctcgaggg ggacgccgtc tgggagcgcg tcagccgcga
    44641 accctcggga acggccttca attcgctcgt ccagctcgag gtcgagcgcg ggatcccgag
    44701 gaacccgttc atcaacgcgg gggcgctcgt cgtcctcgac atggtcctca gccaccagcg
    44761 ggagggagag gacctcgtgc tcgacttcgt ccggcgcgcc ggcgcgagcc ccgacgtcgg
    44821 ctacgacccc gtcgtggccg cggccgagcg ggcgaccggc caccggaacc gcgccctcgc
    44881 ccacttcctg aagtcgttcg ggaacctgag gaacgacccg gaccgcgtcc tcgacgccta
    44941 cttcctcctc tgctcgatct cgatgagctg cgccgagctg gcgtccgcgt tccagtacct
    45001 cacgacgggc gggctcacgg ccggcggcga ggaggtcgcg acggcgcggc aggtgaagcg
    45061 ggtgaacgcg gtgatgctga cctgcggcgt ctacgacgag gcgggggact acgcgtaccg
    45121 ggtcggcctc ccggccaaga gcggcgtcgg cggcggcgtc gtcgccgtga tgcccggccg
    45181 gttcgtgacc gcggtctggt cgccctgtct ctccgagaag ggcaactccc tcgccgggac
    45241 gatggcgctc gagctcctga cgacgaagac gtcgctttcg gtcttctgag gcggccgcgc
    45301 ttccggaccg tgctacggca cgagccgccg cgccgccggc acggaggcga ggagtcgcct
    45361 ttcgagaggg agccgcccgc cttcagctcg agggcgttcc caggcccgaa ccacgcgtcc
    45421 agccccttcc ccgtcgccct caccgcgtag ctggcgaggt cgtcctcgtc ctaaccctca
    45481 cgacggtcac cgcgccggcc ctcgcgtggc tgaaccacca ggcggtccac gcgaccgagg
    45541 tgccggccga gcaccggccg tggcgcgcgc tgaccgcgtg gagcgtctcg gggatcgtct
    45601 tctcgaccgc gttcgccgcc tggtacgtct ggatcctctt ccgctgagag ccacgagccc
    45661 gcgccccgga ggccccgcca gaaggcggcg cctccggggc cgcgtgctca gcagaaggcc
    45721 ggggcaacct tctcgacctt ctcgcgcacg gccgggccca tccggccgag gctacgaagg
    45781 aactcggggt gggtcatccc cgacccgagg aaggtccagc ggctggcctg gcgctgcacg
    45841 gccatcgcct tctcccgctc cccctccgtc agcctccggc cggtcgcgcg ctcgaaggcc
    45901 tcgaggtcga agcgcacctg ctgctcgagg ccggcgtcga agaagccccc gatcgcgagg
    45961 tagccgtcga cggcgcggga gagcgcctcg gcgtccttgc cctccgcgag ggcctccacc
    46021 atgagcgcgt cgagcttggc gtgctgcgcc tcctccatcc aatggtgctt caggaggtcc
    46081 cggaacatcg ggtcgatgcc ggcgtcgtcg cggacggcat cgaggtagtg ccgctgcgtc
    46141 atccattcga tctggaggat cgccagcgcc accgcgagcg gctcgtgggc gaggaccgcc
    46201 ttcgcgatcg cttcgggggg cccgatgacg gcgcactccg ttccgaagcc cttcgtgaac
    46261 tcctcgcgga accgcttgaa gagatggatg tgcttggcct cctcgcccgc gaactggagc
    46321 atcgcccgga cccgctcgtc ctcgcccgac agctgcgttc ggacgtgatc gagcacgaac
    46381 ggcaggatga actcctcgac gagcccgaag atgcagaggt aagagttccc gcggacctgg
    46441 ttcagggtcc gcttctccgc gggcgtgagg aagtcgagag ggtcgacgcg cgcgagcgat
    46501 tcggggagga aggggcggga gaagtcgagc gcgtggcctc ccatcaggtc gtccacggtc
    46561 cagcggacct tctcggaagc ggccaggatc tcgcggtagt cgtaggtcgt cgtcgtgttc
    46621 atctcggtct ccatcgggtg cgggatgtcg tctcgagcgc gcctcagcgc cgctcgcggt
    46681 agaggacgta gtcggccgtg tacgggacga agagcttcgc gccggcggtg gcgcaggccc
    46741 cggcgggagc ggtccctccc tcagtgttga cgcgctggat gtatgaggcc ggggccaggc
    46801 ggtcgccccc ggtcgggccg tccccggtgc ctgtcacccg aaggaggagc cagggtacgg
    46861 ctcccggcgc gacgaagtcg gggtcggacg agctcgcgat cgccatcgcc caggccgcgc
    46921 tggagtcccg cgagtggagc caggtggcgc ggtggatccc gccttccgcg gggctggcgc
    46981 tcaggaagtg cgtcatcacc tgggcggact cgtcgtcgaa caccgtggcc tgcggcccga
    47041 ggaaggtcca ggccgctccg gcctggaggc agacgtagtt ctgcgtgccg acggcgtgcg
    47101 cgaggaggaa cgggcggttc ccgcgcggca cctcgaggcc cgccgggacg gggggaggct
    47161 ccacgtggcc ggcgagcgcg gacggcgcga cgccgagggt caggacggtg gcgatgagga
    47221 ggccgcgggc ggagcccgcc ttcttcgatc gggtcatggt cttctccttg ctcgggtttc
    47281 gagcggtcgg gtcggtggct ggaaggcccg cgcgcgttgc ggggccggct gtcgattcgg
    47341 tcgctacttc cggatcacgg tctcgaggta ttcgccgggg ggacgagcgc cccgcccctg
    47401 ttcacgttcc agcggtcgag gagctcgacc agcgcggccg ccgcgccgcg gccagcgacg
    47461 cgttgccgct tccggccgcc acatcgagga cgcgttcacc cgcgaagacg tcgacggcct
    47521 cgcagagcga ctccccgacg atctgcaacc tcaccccgac ctgtccgaag tcgccccggg
    47581 cccagaccgc ctgctggcgc accttgatcg cctcgtagtc gggcgacgtc ggccgggtcc
    47641 ggggctgagt ggtgtcgatc gcgagatgca tgtcgtcctc ctgcggccgc tctgcggcgc
    47701 gtggtcagcg ggccttcccg gcttcttgtt ccgacttgcc tgttcgtcta cctaaacgcg
    47761 atcgccggaa aatgccgcca atgccggcaa gggattagcg gtcgcgtggt gggtccgggg
    47821 ggggcgtgcg aagcccggcc ccctcggcga gccccgccac cggcgccgcg ctcgcgctcg
    47881 cgcttcgggc agcgcgctcc gccggaggcc tccaggacgg aacgaggagc cggaagagcc
    47941 acgcgccgaa gcagaagccg aacacgaaga ttcccgcgaa cgccaggccg aacgcgacga
    48001 cgacccccca caccacggcc tcccgcccgt cgagcagcgc cagggcggcc gtgaggcaga
    48061 agagagcggg aaggacctgg gcgaagcgtc gcggcgcgcg tgcccccgga aggaagagcc
    48121 gccgggactg ccgccggtgg agcgtcgcgt agagagcgtc gaagggattg aggcgcggcg
    48181 ccgccgcccc gaggagaagg aggcctgcga tcgcggccca gacgaccggt tcgcgcgaga
    48241 gcgccgcccc ggccacgagg acgaccaagg cgcgcggctg gaacgccagg gcgtcgtact
    48301 ggagctccct ggcgtgcgga gcgggctcgg gaagcccctg ctggacgacg aagcggagca
    48361 tgtgcaatgg gctcaaggcg acctcctcct ggagcgccca tgcgctcctc atgaggagag
    48421 ctacgccatc cgggcgggaa acccgccagc tccccctcag tccttccgga ggccgcgctc
    48481 cgggagctca tccagccagc cgaggaccac ccgaagcccg gagggggaag gagtctcgga
    48541 gggagcgggc tgctcgagaa cgaggagacg ccggcgcccg gcgtcgaggt cgaagccgag
    48601 gtagttcggg atgaagagcg atgcgcgcgc acgagccgcg gccggctcct ccgtccagaa
    48661 ggggacttcc tcgtcggcca ggattcggcc ccccttctcg cggagcggca cccgccgcgg
    48721 ctcccactcg ggcgtctgca ggtagaggac ttcgccggcg gaccaccacc tcgggaatcg
    48781 cccgccctcc cgggtcagct ggaccttccc ggagccgtcg gcggggagac gggagacgta
    48841 gacctggacc acggcatcca gctcgtccgt ctcgaaagcg aggagcctgc cgtcgggaga
    48901 gacgactcca ttcgtctcgt tcgcggggcc ggcagccagg tcgcgcggct cgccttcggg
    48961 gcgcaagccg tctccgagcg cgagggcacg caggtcccag ccctgcccgg ggcgctgctc
    49021 ctggaagacg aggacccgac cgtccggagc gaacgacgtc gggtagacgc ggttcgcgct
    49081 ttcgtagagg accgctccgt caccgcctgc ggccggcatg gcgaagaggg cccatcgcgc
    49141 ccccgagcga gcgcccgcga cgagacgcgt gccgtcgggc gaccagatcg ggcgacgggg
    49201 cgagagcccc gtcgtgaccc gggcgagcgt gcccgtcgcc aggtcgagcg tccagagctc
    49261 cgcgtcgtcg gcgcccccta tgcgcacggc caggcgccct ccgttcggcg agagcttcgg
    49321 ctcgcggaaa cggcgcggga caccggggag ccgggtcgcg cgcccagcgg tatcgaccca
    49381 cgccaggtgg ttctcgagcg acgtcggacg gcccgcggcg tagagaagcg tcccgctcgc
    49441 ggagagcgcc accttcgtcc ctccgttcct cgggctgtac gcgacccctt cgaggacgac
    49501 ctctggagcc gatgtcgtga cgagccggac cgggtcgaac gacgcggcgt agagccggcc
    49561 cgcgtgcgcg tagaggagcg gccccctgcc cgcccagagc ccgaacgccg cctcgcgctg
    49621 caccacccgc cgcgccccgt cctcgagcga gaggacctcg aggaccgcgt cgtcccagga
    49681 gctcccctcg gtgagaaccg agaagaggac gtggcgccct ccggggagga cctgcggcca
    49741 gcggtgcgag ctctcgccag tcgccagatc gagcttcgtc agcggctcga ggggcccgcc
    49801 gtccgcccgc acccggtgga gcccggacgt ctgcgagagg ccgacgacga tggccccgtc
    49861 cgccccccag ctccccccgc gcgggttgtt cccgacttcg gcgagagcca ccggagagcc
    49921 gcccgcgagc gggagcctcc agagcttgcg atcggcgaaa taggcgacgg aggacccgtc
    49981 cggcgagaag aagggctgcc gtgccccctc ggtgccggcg agcgggtgcg agtcgaactg
    50041 gtcgagcctc cggaggagga gccgcgtcga cgtcccctcc ctcgcgacga ggacgaccgt
    50101 cctcccgtcg ggcgaaagcg cgaagggtgc cgcgaagctc tcgtcgagcg gctcgccggg
    50161 gtcgagcccg agccgcaccg gctcgacgcg agcaggcccc gaacgcctcg cgacggcgcc
    50221 ggccgcgagg acgatcgcga cgacggcgct cgcggcgagg gcccggcgca ccgcggggcg
    50281 ggccggcggg cgcgcgcgac cgtcccggcg gccgggagcg gcgaccgcct cttcgagatc
    50341 cgcccgggcg tccgcgacgt cctgcaggcg ctctttcggg tccttcttca ggcaacgccg
    50401 cgcgagccgg acgagggccg cgggcgtcgc cggagggagg cggccgaagt cgggctcgtc
    50461 acgcaggacg gaggcgagga tgtccgagac ggtccgtccc tcgaaggcgc gcgtgccgga
    50521 gagcatctcg tagaggaggc agccgaaggc ccagatatcc gtccggcggt cggtctggag
    50581 cccgcgcgcc tgctcggggc tcatgtacgg cgcggtgccg aggatcgtcc ccgagagcgt
    50641 cgtcgccgtc ccagccccat ttccgccgct caccggcgcg gtcttggcga ggccgaagtc
    50701 gagcaccttc acccgcccgt cctccccgag cttcacgttc gagggcttca ggtcgcggtg
    50761 gacgattccc ttgccgtgcg cctccgcgag cgcgtcgacg atctgccgcg ccgcctcgac
    50821 cgcttcgacg gctcccatcg ggcctcgctc cagcctctcg gcgagcgtct ccccctcgac
    50881 gagctcgagg acgaggtacg gcctcccctc cacctcgagg agctcgaaga gcgttgcgat
    50941 gccggggtga ttcagcgccg cgagcgcgcg cgcctcgcgc tcgaacctcc ggaggctcgc
    51001 ggcgtcgtcg tggaaagcgc gggcgagcgc cttgatggcg acctcgcggc cgagctgcgg
    51061 gtccagcgcg cggtagacgt ggcccattcc gcccgagccg agcgagccga cgacccggta
    51121 cggcccgatg gaatccccgg gcgcgaggac gtcctccgcc ggggcgtcgg cgagggagcg
    51181 cgccacctgg gcgaagagcg ggcccgcgac cgcgccgctc ggcgcgaggc ggccgtcctc
    51241 ctcttccgcc caggcgagga ggcgctcgag gcgcctcctc gtctcctcgt tcccggcgca
    51301 cgcggcctcg agaaacgcgg cgcgctcggc aggcggccgg tcgagcgcgg cgtcgaggag
    51361 cccgtccagg accgcccact cgtcgcggtc tggaagctct ctcacggcgc cttctccggc
    51421 gtccctcctt cgagcgcgct gcggagccag gcacgggcct tcatccaccc ccgctgcgtc
    51481 gagcggagcg aaaggccgag cgcctccgag gtttcctcct cgctcatgcc ggcgaagtag
    51541 cggcagtcga cgatcgcggc gagcttctcg tcgtggtccc gcagccgctc gagcgcccgg
    51601 tcgagatcga ggagccactc ggcgtcggcc gcggcggccg tcgcctcctc gtcgaggggc
    51661 accggaggaa cgcccgcccc gcgcttcagc gcgtaccggg cgcgggcctg ccccacgacg
    51721 atctgccgca tcgcgagcgc ggagacggcg agcaggtgtc gtcgatcctc gagccgcagc
    51781 ccgtcctggc cggcgagcct caggtacgct tcgtggacga gctcggtcgt cacgagcggc
    51841 ccggcacctc cgccgcggcg gagctggcgg cgcgcgacct tccgaagctc ctcgtagacg
    51901 agcgggagca gccggtcgaa cgcgccctcc tctccggccg catgggcccg gaggagagcc
    51961 gtcacggaga tgggctcggc caccttgtca ttccctcgag ctgtctctgc ggcgcggatg
    52021 gtagcagccg gccgggcgcg gtcgtcactt cccgtgcgtc gagagggcga cctcctcccc
    52081 cgtgtagggg tcccggaacg cgaggaagac cgcggcgagg cgcatcccgt cgcggttctt
    52141 gttcccggcg ccgtagcgcg ggtcgccgag gacggggtgg ccgatcgact ccaggtgccg
    52201 gcggatctgg tgctggcggc cggtctcgat cgtcacgtcg agcgaggtcg cgccgcgctc
    52261 cgcgtcgtgc gcgacgacac gccagagcgt ccgggcgctc ttgccgtcga gcggcgtgtc
    52321 gacgacgccc tcgcggccga gcgcggaggc gacgtcgccg agcacctcgg cgcggtagcg
    52381 cttgacgacg cgcccttcgc ggaggagcgc cgagagcgcc gcggcggcct gctcgtcgtg
    52441 cgcgacgagg acgagcccgg acacctcgcg gtcgagcctg tggacgggga gcgcggggcg
    52501 gcccagcgcc ttctccgcct ggcggagaag cgtcgcgtgg tcgccgaacg gcgtcccctg
    52561 cgtcaggagg cccgcgggct tgtcccacgc cgaccagcgg acggcgtcgc ggacgaggcg
    52621 cgcctcgggc ggcacgagcg cgaggagggc ggcgtcgtaa tggatctcga ggacgtcgcc
    52681 gggacgcacc gtcgccgtct cgcgccggag gcggttcggc gtccgccccg cgcggccgac
    52741 gtggaccgcc cccttccgca tcgcgtcctt cacgaccccc ttcgagaggc ccgagacgcg
    52801 cgcgaggacg tcggccgcgc ccggagggtc gcccgcgacg acgcggtgcc ggaaggagag
    52861 gcgctcgggc ggagtcatcg gtcccgacga tagcgcggct gcccatgctc tgcggaacgc
    52921 aggcagcgta cgattccagc accaccccgg tcccgtccgg ggagaatcgc cgtcatgcgt
    52981 gccacgtgcc gcctcgtgct tctgctcttc gccacgctcg cgaccgccgg ctgcggctgc
    53041 gtctgggagg gaaggaacgt ctggctcgcc acggaagcct cggcggggaa gacgctcgag
    53101 gcgaacgccc gcgacgaggt gctcctcgag ctgcccgtcg aggccgggtc gaaggcgcgc
    53161 tggtacgtcg tcgaggtcga cgagaaggtc ctgcgccgga aaggcccggc cgaggagcac
    53221 tccggcgggc gagtccggat cccgttcgag gcgctgcgca ccggcgtcac ggacgtcgtc
    53281 gccttctacg cgacagcgcg gagcgcgcct cccgagcgga cgttccggac gaccgtgtgg
    53341 gtccgttgag gtgcgcgccc ggggggaccc ggtgtaacgt tcctccgcgc cttccgagac
    53401 gatgaacggc ttccccctcc tccccgacgc cgagtggctc gagaccgacg gtctcggcgg
    53461 ctacgcgctg tcgaccgtct cggggcggag cgcgcgccgc taccacggcc tcctcgtctc
    53521 ggccacgacg ccgccgaccg gccgcgccgt ccttctcgcg aagctcgagg agacgctcgt
    53581 cgtcggcgac gagcgcttcg acctctcgat caacctctac ggcgggaagc tgcacccccg
    53641 cggagacctg ctgctccgct ccttccgcgt cgacccgttc ccgacgtggg agtacgaggc
    53701 gggaggggcg cgcctgcgcc ggaccttctt cctcgtccac ggcgagccga cggcggtggt
    53761 gacgtgggag ctcctctccg gcggcgctcc cgtccggctg gaggtccggc ccctcctcgc
    53821 ctaccgcgac caccacgccc tccgcggcgt cgagacgccg tgcgacccgg cgctcgtgcg
    53881 ggaagagggc gccgtgaacc tctcgcccgt ctgcgccctc ccggcgctcc ggctcgccca
    53941 cgacgccgcc tccgccacgg agaccttctt ctggtaccgc ggcttcgagt acgacctgga
    54001 ccgcgcgcgc ggcttcgagt tcaccgagga cctcttctcg ccgctcgcct tcgcctggga
    54061 cctcgcagac cgccccgcgg cgactctcgt cgcgacgctg gccggccgct cggccgccga
    54121 tgctcccggt ctcgccgagg cggaggagag gcggcgtagg aaggtcgagg gggccggctc
    54181 cgaccgccgc gccctgcgcg tcctccggaa cgccgccgac cggttcgtcg cgcggcgggg
    54241 cgaggggtgg acgatcgtcg ccggctaccc gtggttcacc gactgggggc gcgacgccat
    54301 gatcgcgctc cccggcctcc tcctcgccac gcgccggttc gacgccgccc gctcggtcct
    54361 cgagacgttc gccagccacg tcgaccgcgg catggtcccg aaccgcttcc ccgacgaagg
    54421 gaccgagccc gagtacaaca acgtcgacgg cacgctctgg ttcgtcgagg cggtccgcgc
    54481 ctaccacgag gcgacccgcg acgacgcctt ccgcaggcag gtctacccgc tcctcgtcga
    54541 gatcgtcgag cggcacctgc gcggcacgcg cttcggcatc cgctgcgacg tggacggcct
    54601 cctctcgtcg ggcgagccgg gccagcagct cacctggatg gacgcgcggg tcgacgggcg
    54661 cgtcgtgacg ccgcgcgccg gcaagccggt ggaggtgcag gcgctctggc acaacgcgct
    54721 gcgcgtcgtc acgctctacg ccgccgacct cggagacgcc ttcacggcgg cccgcctcct
    54781 cgcgatcgcc gaccaggcgc gcgagagctt ctcggccctc ttctgggacg agggggtcgg
    54841 ctggctcgcc gacgtcgtcg ggccggacgg gacgcgcgac ctctcgctcc ggcccaacca
    54901 gctcttcgcc ctctcgctcc acaggcccct cgtcaccgga cggcgagccg agcgggcgct
    54961 ttcggcgatc gaggagaagc tcctcacccc gtacggcctg cgcaccctcg accccgcgca
    55021 ccccgactac cggggccgct tcgagggagg gccggcggag cgggacgccg cctaccacca
    55081 gggaagcgcc tggccgtggc tcctcggcgc tctcgtcgac gcccacctgg ccgtccgcgg
    55141 gaacgggccc gcgagccgca ggagggcctc ggcgtggctc gccccgcttc tcgagcatct
    55201 cctcgggccg gggctcggcc agcttccgga ggtcttcgac ggagacccgc cccaccgcgc
    55261 gggcggctgc gtggcccagg cctggagcgt cggcgaggtg ctccgcgccg cgctgaagac
    55321 gggcggctga gcgggcgctg ggtccgggcc gagccccccg gaaaccctcc agagcttctc
    55381 gcggcggttc aggccgaagg acgcgacggt gccggcggcg cgcgcccgcg ctcgggagtg
    55441 cccgtgccgc gcttctcagc tcggcatccc ccgagcgcct gcgcgctcca gtagtcaggg
    55501 ccaaccccct ctcagtcggc ttgacgaaag cactgaggcc aatgcccgac actggccgac
    55561 tagaatcggc ctgtagtgaa agcacacgcc gcgaccgccg cacggccgtc cccgctggcg
    55621 cttccgctgg aagggagtcc cgcggaggaa gccgcccttc gcggcgggct gcaccttgag
    55681 gagcagacgg ccgagcggcg gcgagagctg gggcagttct tcacgccggt gcctgtggcc
    55741 cgcttcatgg cgcgcctggc ccgcccgggc cgccgcgtcc gcaggatcct cgatcccgga
    55801 gcgggctccg gggtgctcgc ctgcgcgctc ctggaggagc ttcccgacgg cgccggaccg
    55861 gtccacgtgg acgcctacga ggtggaccac ggcctggccc gcgtctgtca cgactcgctc
    55921 tcgcgggcgc tggcgtggct cctgggtcgc gggatcgaga tgacgttcaa ggtgcaccag
    55981 acggacttcg tccgggcgaa tgccgcgtgt ctctcgacgt cgctcttcga gagcgcgacc
    56041 cccgacccgt acgacgtggc gatcctgaat cccccgtact tcaagctcgc caaggccgat
    56101 ccgcgcgccc gcgcggcggc cgagatcgtc cacgggcagc cgaatatcta cgcgctgttc
    56161 ctggcgatca cggcgagcct gctggccgag aagggggtga tggtgagcat cacgccccgc
    56221 agcttcacga ccggtgacta cttccggagc ttccgcgagc acctcttctc gcaggtgacg
    56281 cccgagacgg tgcacctgtt cgattcgcgc caggacgcgt tctccaagga cgaggtcctt
    56341 caggaaaacg tgatcgtgcg ggcccgcaag gccaagcccg caccggacgc cagcgtgcag
    56401 gtgagcacga gcgcgggggt gtcggacctg ggccggccga ggacacggac ggtgccgctg
    56461 gagtcggtgg tcgacctgcg ctcacgcgac ttcgtcctgc acatcccgac cgccgagacg
    56521 gacgacacag tgctttcgtt cgtacgcgcc tggcctcaga cgctcggctc tctcgggctg
    56581 tcggtctcga cggggccggt ggtggcgttc cgggcacggg agagcctgct gtacgaggcg
    56641 gacgccgctg aggaggcggt gcctcttctt tggcttcagc acgtgcagtc gatggcggtg
    56701 cgctggccga tccccggtgt ccacaagccg cagttcttcc gcgtggagcc ttcgacacag
    56761 aagctcctcc tgccgagcga taactacgtc gtgatgcgga ggttctcggc caaggaggag
    56821 gcgcgccggc tggtggccgc gccgctctac ggacgcgagc tctccgagag cacgctggct
    56881 ctcgagaatc acctgaatta catctaccgc ctccgcgggt cgctgacgcg cgaggaggcg
    56941 acggggcttt cagcggtgct cggaagcgcc cttctcgatc gttacttccg cgtctccaac
    57001 ggcaacacgc aggtgaatgc gacggagctg cgcgcgctgc ctcttcccgc tctccgtgag
    57061 atcacggcga tcggcgagga gctgctcgag agccccgacg ccgccgcgcg tgccgaagag
    57121 gtgatgacgc ggatcctcca cgtgcccgcg accctgcggg ccgagatcgg acggcatggg
    57181 taagctcgaa gacggaaaga agatcctcaa ggcgctcggc cttccccctc agcagcagac
    57241 ggacatcgcc tcgtacacgc tcctcgctct cgccgacgtc gggaagcggg tgccctgggc
    57301 ctcggcggac cggcggtcga tgaagatcca cgcgatcaag gagtgggtcg gcgagacgta
    57361 cgggaaggtc tacgcagaaa acacgcggga gacgttccgg aggcaggtgc tgcaccagct
    57421 cgagcaggcc cgcgtcgtgg accgcaatcc cgacgatccc actctgccgg tcaacagccc
    57481 ccggactcac tacgccctga ccgccgatgc gctggcggtg gtacgggcgt tcgggacgag
    57541 cgccttcaag gccgaggccg agaagttcgg gaagagtcac ggcgcccttc tcgagatgta
    57601 ccgcgccgcg cgcgtcggac aaagggtggc ggtggtcctg gcctccggca agaagttgac
    57661 gctctcgccc ggcgagcaca acgagctgca gaaggcgatc gtggaatcgt tcgcaccggt
    57721 cttcgctccc ggggcgcgcc tgctctatct cggcgacacg gccgcgaagg acctccacgt
    57781 ggagaccggg gctctcgaag cgctgaagat ccccgtgacg aagcacggca agctccccga
    57841 cgtcgtcctc cacatgccgg agaagggctg gctcctcttg atcgaggcgg tgacgtcgca
    57901 cgggcccgtc tcgccgaaga ggaagaagga gctcgagctc gccctgaagg actgctctct
    57961 gccgcgcgta tacgtcagtg ccttccccga tcgggacgaa taccgccgcc acatggccga
    58021 catcgcctgg gagaccgagg tctggatcgc cgacagcccc acccacatga tccattacaa
    58081 cggcgagaag tttctcggac cccacgcgcc gtactgagac atgacgcgcc gacttgcggc
    58141 ttccgcaacg gacagcatgg cccccggctc gcacgattcc atacccgaga gggcacgatg
    58201 agcctgcgtt acgagagctt cccaaagacg atcctggaga tcatccactc cttcgagaag
    58261 gggcgcttga atctctcgcc aggcttccag cgggactccg tctggaccga cagcgaccgt
    58321 cagaagctga tcgagagcat cttgaggggc taccccctgc cgtccgtctt cttctacaag
    58381 cgcgagaccg acggccagat ctacttcgac gtcatcgacg gcaagcagcg gatcgagacg
    58441 atcctgaggt tcacggggcg gatccggggg aaccgcttcg ccacgaagaa gatccagctt
    58501 ccgggcgagg acgagaagga ctgggtcgac tgggacaagc tcaagcgtcg ccggcagcag
    58561 cacaagatcg agagctacac gctcctgacg atccaggtcg acggcgagct gtccgaggtg
    58621 atcgacctgt tcgtgaagat caactcgacc ggcaaggccc tctccgccgc cgagaagcgg
    58681 aaggcgaagt tctacgacga cgacttcctc aaggaggccg cccgcctggc gcggaggttc
    58741 gaggggtact tcaaggctca gcacatcctg agcgtcggcc agatcctccg catgaagaac
    58801 gtcgagctga tgtgcgagct gatgctctcc atcagcaagg gcgacgtgat caacaagaag
    58861 gccgccctgg accgtgtcat gcagtccggg agcatgacgg cccaccagct cgagaaggcc
    58921 tcccgactga cggtgagcgc tctcaaccgc gtccgccgac tgttccccga cctgggtcgg
    58981 acgcgcttca gcaagatctc cgacttctac tcgctcgcgg tcctcgtggc ccggctcgag
    59041 agcgaggggc tgatcctgac cgatcggcgc cggaatcggc tcgcgtggga cctgctcgtc
    59101 tccttctcga atggcgtcga cacggtgcgg gagaggcagc gtcgcgcgga gggggccggc
    59161 cccggggagg agctgtaccg cgagtacctg ctgaccgtgc tggaagggac cgacgagatc
    59221 acgaagcggc agaaacgcga ggagatcctc cgcggacttc tcgggagcct cttcgagagg
    59281 accgacgagc ggcgactctt cagctcggag cagcgccgca tcctcttcaa ctccgcgacc
    59341 gatcgaaagt gcacggcaaa ggggtgtggc cggcggctcg gctgggacga tttcacggcc
    59401 gaccacgtcc gcccgttcag ccagggcggc cgcacggtcc tctcgaatgc cgcccttctt
    59461 tgccgcaagc acaattccgc gaaggggaat cggcgcccgc atcgaggggg acgacgtagc
    59521 cgttgaggct ggcacttcgg gccgggcctc ggccagctcc cggaggtctt cgacggagac
    59581 ccgccccacc gcgcgggggg ctgcgtcgcc caggcctgga gcgtcggcga ggtgctccgc
    59641 gccgtcctga agacgggcgg ctgagcgggc cggtcagtcc cggccggagc cgctcccggc
    59701 tccccactcg gtgtggaaca gctccccgcg cgggcggtcg acccgttcgt agcggtgcgc
    59761 gccgaagtag tcgcgctgcg cctggaggag gttcgcggga aggcgcgcgc tccggtaccc
    59821 gtcgtaccag gcgagggcgg ccgagaggcc gggagcccag acgcccgaaa gcgccgccgc
    59881 cgagacgacc cggcgccagg cgtcctgggc ctccccgacc ttgccgcgga accaggggtc
    59941 gaggaggagg ttcgggagcg ccggctccgc ctcgtaagcc tccttcacgc ggtcgaggaa
    60001 ggacgaccgg atgatgcagc ccgcccgcca gaggagcgcg atccctccgt agcgaagccc
    60061 ccatccgcgg ccctcggagg ccgcccgcat cagggcgaag ccctgcgcgt aggagacgag
    60121 cttggcggcg aagagcgcct tcccgaggtc gtccacgaag gccgcgcggt cgccggcgaa
    60181 ggagggcgac ggacccggga ggacggttga ggccgcgacc cgctcctcct tcagagtcga
    60241 gaggaagcgg gccgagaccg ccgtgtcgat caggccgagc ggcacgccga gctcgagggc
    60301 cgtggcgctc gtccagcgcc ccgtcccctt ctgccccgcc acgtcgagga tcgcgtcgac
    60361 gagcgggcgc ccgtcggcgt cgcgcgccgc gaggacgtcg cgcgtcacct cgacgagata
    60421 gctgtcgagg tcgccccggc tccatccgtc gaagaccccg ctcatctcgt cggcgtccat
    60481 cccgaggagg cgcgacatga ggtggtaggc ctctccgagg agctgcaggt cgccgtactc
    60541 gatcccgttg tggaccatct tcacgaagtg cccggcgccg tcgctcccga cccagtcgca
    60601 gcagggggtc ccgtcgggtg cccgcgcggc gatcgcgcgg aagaggggcg cgacgagcgg
    60661 ccacgccgcc gccgagccgc ccggcatgat tgacggcccg aggagcgccc cttcctcgcc
    60721 cccggagacc cccgcgccga cgtagaggag cccgcgcgcc tccagctcgc cggcccgccg
    60781 cgccgagtcc tcgaagcggg agttcccgcc gtcgatcagg acgtcgcccg gcgagaggtg
    60841 cgggacgagc gcgtcgatca gcccgtcgac ggccgcgccc gccttcacca tcagcatcac
    60901 gcggcgcggg accgagagcg cggcgacgag ctcggagacc gaccgtgcgc cgacgacgtt
    60961 ccgccccttc gcccgccctt cgagaaacgc gtcgaccttc tcgaccgtcc ggttgtggac
    61021 ggccacccgg aacccccggc tctcgaagtt gaggacgagg ttctcgccca tgacggcgag
    61081 gccgatcagg ccgatgtccg cgcgtccgtc cgccattcct cgcctcctcg gccagccgag
    61141 cttcatccga ccgcggcggc cgcctcgatg atcctccatt cgcgcgtctt gtccgcggcc
    61201 cggccggttc gcgaggatcg ccttcgtctt ctcctcccgg tgctctccgc gccgtttgac
    61261 accctcctcg cgtctgttat ggttcgccgc tgcaaggaga tagcggggag gagcgatcct
    61321 ctttcgccgg gaagaaggat gccgactcgc ctcgaagagt ccctcggacg cgacatcgac
    61381 cggatccgcc agcagctcct cgagatgtcc gccctcgccg agcggaacct ccgcgactgc
    61441 gtcgaggcga tgaagggggg cgagcgggcc cgcgcctacg ccgtcatcct gcgcgaccag
    61501 ctgatcgacg agaaggagaa ggagatcgac cgtctctgcc tcgagttcct cgtgaggcag
    61561 cagcccgtcg gcttcaacct ccgcctcgcc tacagcgcga tcaagatcaa cctcgagctg
    61621 gagcgggtcg gcgactacgc cgagagcatc gcccgccagg tgctccgcct cgagaagctc
    61681 ccggcgacgc ccgagaccca caccgggcgc ctcgtcgaga tggccgacct ggcgatcccg
    61741 atgatccacg acgccacccg ggcgttcctg gcccaggacg ccgacctcgc ccgcgcggtc
    61801 gtcgcgcgcg agccggcggt cgacgcgctc ctcgggacgc tcgtcaccga gctcctcgac
    61861 gaggtgagcg cggggcggat cggggccgag atgctcgtgg ccctctcgac gatcggccgc
    61921 cgcttcgagc gggtcgccga ccaggcccgg aacgtctgca tggagacgct ctacatgtgc
    61981 accgggcagt acgcgaagca ccccggcgcc gagacgttcc gcgtcctctt cgtcggcgag
    62041 cacaacgcct gccggagcca gatggccgag gcgatcgggc agtcgctcga ccagagccgc
    62101 ttcctcttct cgagcgccgg cctctcgccg cgcgcgatcg acccgcgcac ggccgagttc
    62161 ctcacggaga agggctacga cgccgcgcgc atggcgccga agggaacgga ccaggtcccg
    62221 aacctcgact actacaacgt catcgtcggc gtcgcgcccg aggcgcagag cctcttcccg
    62281 cgcacgccgc gcaacctcgt ctacctcgac tggagcaccg ccgacccgtc gcgcgccgcg
    62341 ggctcccccg aggaggtccg cgccgcctac caggccacct acgagttcct cgaacggaac
    62401 atccgcgacc tcgtcggcgc cgtcctcgac ggcacgcacg actgacggcg ggagccgcgc
    62461 ccggagccca tgccccgagc cgcattcgca agaccgctcc tcctcctcgc cgccgtcgcg
    62521 gcgctcgggc tccccgcctg cggacgccgc gacgggggag cgtctccctc gaacgtgatc
    62581 cagaacaagg gctccgacac gatggtgaac gtcgcccagg tctgggccga ggcgtaccgg
    62641 aaggcggcgc ccgacgtcga ggtcgaggtc tccggcggcg gctcgggggt cgggatcgcg
    62701 gcgctcgtga aaggggccgt cgacatcgcg aacgccagcc gcgacatcaa gccgagcgag
    62761 tccgagatgg cgctcgcgaa ctcggggaag gcgcccgtcg acttcaaggt cggctacgac
    62821 gccctcgccg tcttcgtcca ccgcgacaac cccgtctccg agctgacgct cgcgcagctg
    62881 aaggagatct acgccgaagg ggggcgcgcc ctccgctggt cggacctcgg cgtcacgatc
    62941 cccggcgttt cggacgaccg gatcgtccgc gtgagccgcc agtcgagctc cgggacgtac
    63001 gagttcttcc gcagccgggc cctcgacgac gaggacttcc gcctcggctc cctcgacctg
    63061 aacggctcga aggaggtcgt cgagctcgtc gcggtcacgc cgaccgcgat cggctacagc
    63121 ggcatggggt acgcgacgga ccacgtgaag atgctccgcg tctcgcccgg gacgggcgtg
    63181 ccgggcgtcg ggccgacggt cgacgccgtc cacgacaaga cctatcccct cgcccgctcc
    63241 ctccacctct acacgctcgg gccgcccgag ggcgccgtga aggactacat cgactggatc
    63301 ctctcccccg ccggccagga ggtcgtccgg gtggcgggct acgtcccggc cgtcgtggct
    63361 ccggctccgg aggccgcaac ggccccgccg gccgccccgt cctctcccgc acccgctcct
    63421 tccgccccgc cggctccgga ggccgccccc gcacgatgag cgcttcgacg accgctgcgc
    63481 cccgccccga gcggccccgc ctgtccgtcc tcggcgagaa ggcgctcgag acgctcatcc
    63541 gcctcgccgg cgtgagcgcg atgctcttcg tcgccgccat cttcttcttc gtcctgcgcg
    63601 aggccttccc cgtcctcgcg aacgagaagt tctccctcac gcagttcctc ttctcgaccg
    63661 agtggtaccc gacctcggcg aagaacgtcc gatacggcac cttcgcgctg acgatcggga
    63721 cgctcagcgt caccgcgctc gcgatcctca tcgccgtccc gttcggcctc ggggccgcga
    63781 tctacctctc cgagttctgc gcgccgcgcc tccgcgagac gctgaaggtc gtcatcgagc
    63841 tcctctcggc gatcccgagc gtcgtctggg gcttcatcgg cctcacggtc atgagccggc
    63901 tcgtcgtctc ggtgaccggc gccccggtcg gcgtgaacct cctgaacggc gggatcctcc
    63961 tcgcgctgat gagcgtcccc gtgatcgtct cgatcggcga ggacgcgctg aaggcggtcc
    64021 ccgactcgta ccgcgaggcg ggcgtcgcgc tcggggcgac gcgctggcag ctcgtctggc
    64081 gcgtcctcct gcccgccgcg aagaacggcc tcctcgcggc cgtcctcctc ggcgtcggcc
    64141 gggccgtcgg cgagacgatg gccgtcctga tggcgaccgg ccacgcggtc aacatcccgg
    64201 gcgggatcct cgagcccgtg cggacgctga ccgccaacat cgccgccgag ctcggcgagg
    64261 ccccgtcggg ctcgccccac taccgcgtcc tcttcctcac cgggacgctc ctcttcctca
    64321 tcacgttcgt ggtcaacctc gccgccgact tcgccgtccg cggcgtgcgg aggaagtgac
    64381 ggtggcgggc cgcttcgcga agaccccgca cggcctgcgg aaggagcgga cggaggcgct
    64441 cgccaaggcc gtcttcggcc tgatggcctc ggcgatgatc ctgccgctcc tcctcatcgt
    64501 cggctacctc gtcgtcctgg cctggcccgc cctcgggtgg gagttcctcg tcgacgttcc
    64561 gaagaacggg atgcgagacg gcgggatctg gcccgccttc gtcgggacgc tctacctcgt
    64621 cgggatctcg ctcgtcgtct cgaccccgat cggggtcctg gcggcgatct acctgaacga
    64681 gtacgcgaag gacagctggt tcaaccgcct cgtgaacctc gccgtcatca acctcgcggg
    64741 cgtcccgagc atcgtccacg ccctcttcgg gctcggggcg ttcgtctacg ccgcgagatt
    64801 cggctactcg atcctctccg cctcgctcac gctcgcgatc atgacgctcc ccgtcatcat
    64861 cgcgtcgacg cgcgaggcgc tcgcctcggt gccgacggcc ttccgcgagg catgctggaa
    64921 cgtcggcgcg acccgctggc agacgatccg gaccgtcgtc ctcccgaact ccatcagcgg
    64981 catcctgacc ggcgtcatcc tccaggtgag ccgcgcggcg ggagagacgg cgccgatcat
    65041 gttcacgggg gccgtcttct tcaaggccgt cgagaagggc gacctcttcc cctacggcct
    65101 cggcgagcag tgcatggcgc tctcgatgca cctctacacg ctcgccaccc aggtcccgaa
    65161 cgtgaaggag gcgctcccgt acgcgacggc ggtcgtcctc ctcgcgacgg tcctcctcgt
    65221 gaacgcgacg tcgatcgccg tccgcatctg gctgaggggc cggaagaagt ggtagccggg
    65281 agcgccgccc cgctcgagat cgaggtccac gaccttcggc tctcgtattc cgggcgcgag
    65341 gtgatccacg ggatctcctt cgaccttcga aagaacgaga tcctcgccgt catcgggccc
    65401 gcgcagtcgg ggaagacgtc gatgctccgc tgcctgaacc ggacgatcga cttcatcccg
    65461 acggcttccg tctcggggac cgtgaaggtc cacggggagg acgtgcgaag gacgaaggac
    65521 gtcttcgctc tccggcggcg gatcgggatg gtcgccccgc tcccggtcgg cctccccctc
    65581 tcgatctacg acaacgtcgc cttcgctccc cgctgcgcgg gagtcacgaa ccgggcggac
    65641 ctcgacccgg tcgtcgagaa gtgcctgcgc caggccgccc tctgggacga ggtgaaggac
    65701 cggctcggga gcctcggcac cgccctctcc ggcggccagc agcagcgcct gacgatcgcc
    65761 cgcgccctct cccaccgccc cgagatcctc tgcctcgacg agttctcgat cgcgatcgac
    65821 ccggtgacga cgatgcggat cgaggacgtc ctgaaggagc tgaaggaaga gatcacgatc
    65881 gtcctcgtca cgaacctgac acagcaggcg cggcgcctct cggaccggac gatgttcctg
    65941 ctggagggcg agatcg
//
LOCUS       DAOONL010000011        58018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_354_length_58018_cov_21.444249, whole genome shotgun sequence.
ACCESSION   DAOONL010000011 DAOONL010000000
VERSION     DAOONL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 58018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58018
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_58018_cov_21.444249"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..333
                     /locus_tag="PLP50_03240"
     CDS             97..333
                     /locus_tag="PLP50_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50593.1"
                     /translation="MSHAPASAASSWLKQWQAAGPALEEVRRRELRELPDDEALAAAE
                     ALLSLAASSPLPEERRISSGLVTQQALLHRRRPR"
     gene            330..908
                     /locus_tag="PLP50_03245"
     CDS             330..908
                     /locus_tag="PLP50_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50594.1"
                     /translation="MNPIFAAAREIQDFCRGRGWRSCVIGGLAVQRWGEPRLTRDVDV
                     TLLTGFGAEASYVDGLLSAFRGRLSDAREFALRNRVLLLSSSTGTPIDVALGAMPFEE
                     AAVSRATPFRMGPDLEVLTCGAEDLVVFKAFAGRPRDWLDIEGIVARQGTRLDAPLIW
                     RELLPLLELKDDGETEGRLRRLLASSATGDTG"
     gene            complement(948..3464)
                     /locus_tag="PLP50_03250"
     CDS             complement(948..3464)
                     /locus_tag="PLP50_03250"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPA50595.1"
                     /translation="MTEQRVPGPGDEAESVSRARVEGWKRAIQDSLVTAVGRFPAMAT
                     RNDRYLAVAHAVRDRLVRRWMRTGEHYYRQRVRSVCYLSAEFLLGPHLGNALVALGVL
                     DEVRQAVEEMGWGFEEVLAQEEEPGLGNGGLGRLAACFMDSLATLRIPAIGYGIRYEF
                     GIFDQEIRDGWQVEKTDKWLQYGNPWEVPRPEIAFEVKLGGRTEAFTDESGRYRVRWV
                     PAQVVKGVAYDTPIPGYRVGNVNLLRLFKAEAAESFDFAAFNVGDYYGAVEEKVGSET
                     ISKVLYPNDEPAAGKALRLSQQHFFVSCSLQDMIRIHLQASPDLADLADRWAVQLNDT
                     HPAIAVAELMRLLVDEHGMGWDEAWSITRATFAYTNHTLLPEALEKWPVALFGKTLPR
                     HLEIVYEINRRFLDELRARFPGDEGLAARLSLVDEGGPRSIRMAHLAAVGSHHVNGVA
                     ALHSELLRRDVMRDFAAIWPEKFTNVTNGVTPRRFVALANPGLTRLVTEAIGDGWVRD
                     LDELRRLEPLADDAGFREAWRRVKAANRAALAEAARQRAGVSADPASLWDVQVKRIHE
                     YKRQHLNLLHVLTLYRRLKRDPGLASPPRTVLFAGKAAPGYATAKLMIKAIHSVAEVV
                     NGDPAMKGRLAVVFWPDYNVKSSLPIFAGSDLSEQISTAGKEASGTGNMKLALNGALT
                     IGTLDGANVEIREAVGAESFFLFGLTVEEVERTWRDGYRPGERYEADSELREAVDALV
                     SGEFTRGDRGLLRPLVHGLLTSDPFLVLADYRAYVEAQGEVEAAWRDPERWARASILN
                     TARMGRFSSDRSIRDYAREIWKAVPAPVPVVEGEERPPCG"
     gene            complement(3674..5032)
                     /locus_tag="PLP50_03255"
     CDS             complement(3674..5032)
                     /locus_tag="PLP50_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA50596.1"
                     /translation="MKATWRILVIDDEAVMRESLAAWLREDGYEVDTAASGREGIDKA
                     READYAIYFVDLKMPGGIDGIETMMEVKKLRPDASIVIITAYATVDTAITAMKEGALE
                     YIVKPCNPQEISLLVTRIIKVRNLERENEILRRSLTGRWRFHDLLSKNPRMQEIFGLV
                     RDVASLKSTVLITGESGTGKELVARALHFSGERAEGPFVGVSCAALTETLLESELFGH
                     EKGAFTGAVERRKGKFELAEGGTIFLDEIGDISAKVQVDLLRALQERRFFRVGGSAEV
                     AVDVRVVAATNADLAEEVRQGRFRDDLFYRLNVVAIHLPPLRERPEDVPLLARSFLER
                     LSHELGKEAEDLSEGALKALLAHRWPGNVRELENAVERALVTAKGRVLTEDDFAFLRP
                     VSEAAPGWSVPTHLPLSEVEKTVIAATLKRNDGNVKETAAVLGIDRSTLYDKLKRYGI
                     ER"
     gene            complement(5029..5592)
                     /locus_tag="PLP50_03260"
     CDS             complement(5029..5592)
                     /locus_tag="PLP50_03260"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HPA50597.1"
                     /translation="MSVLAVVPIGPSDDEVLAAVEGVVATAFGRETARLAPIPEPAGA
                     FDAARGQWSAPEMLKALLSARPPEADRILGVTGRDLFVPVLSFVFGQAQMGGPTAVIS
                     LARISQAFSGLPEDRPLLLARARKEAVHELGHTFGLVHCPDPACPMSLSTGVVQVDRK
                     GESLCASCAALVKERTPAARPPEGRTG"
     gene            complement(5589..7199)
                     /locus_tag="PLP50_03265"
     CDS             complement(5589..7199)
                     /locus_tag="PLP50_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50598.1"
                     /translation="MRGPRRLSQKLILSLTVVVSLVVVANGVIAVRSAETQLQATMLQ
                     SVEQLSGAIASSTWHAMLADQRDAAYEVMQTIAAKQGIRSVRIFNKEGRVMFSTPRSE
                     VKVVDKKAEACFLCHASEQPLVRVDAPTRARVVHRPGGGRTLAMVTPIYNEPACSNAA
                     CHAHPESQNVLGVLDVGLDMKLVDQQVRRIEIRTALFTSLAVLLVGVCAAAFTRRFVT
                     TPLTRLSEATRQVAEMKLDRPIALTSSEELEALASSFNEMRRRLALAMEELNELTRGL
                     EEKVDERTEQLKVAQQKLMQADRLASLGQLSASVAHEINNPLSGVLNLSMLMQRILKD
                     DGVPKGREAEFRRYLEQVVAETARTGRIVSDLLAFSRRGTPVRSETDLNVIVGRTLSL
                     VSHKLKLMNVEVECALDAALPRVEADSSQLQQVVMNLVLNAGEATRRKGAGHLRVSTR
                     ATALKKEVVLEVRDDGEGMPPELKERIFEPFFTTKDEGKGVGLGLAVVYGIVQAHDGD
                     IEVESAPGEGTLFRVTLPVRGEAAAPKA"
     gene            complement(7330..9414)
                     /locus_tag="PLP50_03270"
     CDS             complement(7330..9414)
                     /locus_tag="PLP50_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50599.1"
                     /translation="MRIVLAAAVLAASFSLGGSVSAAAVPSVSPDALKSLEAELVERH
                     GEAARPRITRGLAQAAALWRVSDGNDAAFADVAKTHFLSDPAQLDALFSRMERVLESV
                     GGHMLEIVRDLREHSDLDRGEILPFDDVMAGWDPSAHVADDLFENRLAFAVLLNFPLT
                     TLPERLEKGPSWSRREWAETRLAEGFGKRIPAEVNQAVATASADASRYISEYNLRVHH
                     LLDEKGRRLFPQGKRLLSHWNLRDEIKACYGDEKDGLAKQRTIARAMERIVNQTIPTS
                     AIDSPWIDWNPFTNEVKATTSLEPGAPPVPKDLPVFVSPESVGRYAKLLATFRAARLV
                     DPYSPSAPTLIARRFQEDRQLPEARVRAMLESVLFSPVFAQVGALVKERLGRPLEPFD
                     IWYPGFRDRGPYTEAQLDEITRRKYPTPEAFEKDIPRILRDLGFPEERARAIAANVVV
                     DPARGSGHAWGAARRGDKAHLRTRVGKEGMDYKGYNIAVHELGHNVEQTISLNDIDHT
                     LLAGVPNTAFTEAIAFVFQERDLPLLGLPKGDAKGRALATLNTYWGTAEIAGVALVDM
                     NVWHWMYRNPEATPAQLHQAVLASAKNVWNRFYAPVFGVKDSTILAIYSHMIDSFLYL
                     PDYPLGHLIAFQIERHVEKAGAVGPEVERMARQGRISPDLWMKGAVGAPVGSEALLEA
                     AAEALGEIGRGR"
     gene            complement(9473..10981)
                     /locus_tag="PLP50_03275"
     CDS             complement(9473..10981)
                     /locus_tag="PLP50_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50600.1"
                     /translation="MDSAAGSPYHPDGGDVRDDERRDAADLPELLEILGEVSERVGAL
                     SLSRRSHAGAEEIEELRERLLFARHLLEGLFALRAEPVPDPEPGVAFEALGPGLRRTL
                     PQRLAAQLEDERNAVTERSLQELSSFAAFGLHARVVELLRLVDWVTKLAAEEARRAAP
                     PTVVSLRHAPRAAAKGSARPAPPDAAPAGRPPEAAPPAHRPAPPAAPREDPGPDLSAY
                     DLRIDEAGERVDVAGASAWFASAAGPRAEGPGLAASFAVAFPGGIALAVATGAESSLG
                     SRLAAVVAARTFCRAAAAGPSDPLAAVRTAQGHLEMLLAALMTAGDSSDALTRVRGTM
                     ASDGARRVLAHTRAPDPALRRVPPALACGLVGAIATVSGGRVEAKVVRLGPGTVQARR
                     GGAPSPVPFARSPEPAFLAPGTLGAGGLARAEAVPAVTLARGDALLLATPAVGRGAPS
                     AWTSLATLWPPFPDGLGDGASARKLLDRAARWGVAEPPSFGGPLGIGVLLAR"
     gene            11018..15592
                     /locus_tag="PLP50_03280"
     CDS             11018..15592
                     /locus_tag="PLP50_03280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA50601.1"
                     /translation="MSRGPSLPDSLDRWERAPSPERAAELLEAARAALSEGSAPPALW
                     HRWLDATRRSEHLLALGDRETRVVWAEVAIGAVHASGYDLARLLAQRVAAHPERAFLV
                     DGPAPGAPRWSYAHVARRADAIAAALLALEAEPRVAIVAENGVDSACLDLCCLLHGLL
                     VAPLSPHLMAGELVAAFDALGVNLAVVASEESLRRVREAALRTARPFRVVLLDEAAEL
                     ASERDVVLGETLAAQAEASAPALLARAPRRALHETCTVLFTSGSTGGQKGVAFSMANL
                     VTKRFARAAALPFVGEEETLLCFLPLYHTFGRYLELLGMLFWGGTYVFAGNPSLDGLA
                     AGLRAVEPTGLISIPIRWAQLESRAREAAGEAPSPEASAAALRSVVGGRLRWGLSAAG
                     YLAPQVFRFFQAHGVELCSGFGMTEATGGITMTPPGAYEEGSVGLPLPGVRVRLAEHD
                     ELQIAGAYVARYLDEPETAPGEERWLPTGDVFRVRPSGHLEIVDRVKDIYKNSRGRTV
                     APRRVESRFEGVPGVRRVFLAGDGRDDNVLLVVPDAEDPVLAGDPHGETARDYLRRIV
                     AAANADLAPYERVVDFAVLPRDFSLEEGELTPKGSYRRKAIEKAFAPVIEGLYRNRVV
                     RLAGDGVEVRVPRWLLRDLGLLEGDLAVEGGRLVDLRHGRSAALAARGEGEEVRVGSL
                     AYRCAGQAVDLGLLARQPLLWLGNPELAALLPVKDGWDTPLTGSCPEVLLPSREEVPE
                     GADAPLPAPLPSPQLTALHKLSVALLFGPPEGSLAAVARAEALLRQGDRRTAELLRRR
                     LQALARHPDVRVRCEAYRVLLLDDPLVDPGTILPAFLRSGLPFLSEESVAAIARAELG
                     EERLTALRRRLHSYREAMTGPLPETTRSTLGDVLELLGDFARHHPEDHAAVRAELASW
                     AIQERDPELARRADALFDALGVWHEETAAAAALLPPVSEGRKKLSFRDGMPEEEVRTL
                     EGLLLGTSFLRESIELAFETDPPELADIPDEGIWVTPVQALQRHRLYRLSVRTASGNH
                     HDLLLTVRGDLDSAAVRATTLRMIALAGHPWGPPVVPRVGSCRPDLGALSLAWVDELT
                     LAERLRDAAVPGRARPGEGHSVKSLVVRALAAFFTAWDRSGRLLVPGLVAPSNVAVPA
                     EDYRDGARLLSISGARAYTGPLDLVAPMLRNAFREPAALSPRLAAVLSPAWIVDAVFE
                     ALGEAGARTFLTELREAAGAPSSEAWERLRTAVDAALAAPPGDRTPLALETAVARYEA
                     WEGERPGVSLAERERTAESLLRLYRVDALGEEARYRLFRATVFARSAPPVLRAFDALL
                     AALRARPGSRPTHLVELQGLHATLSTDAERHAFTRLVFPAARSTRPVEVSVVGDAVKH
                     VVVATELVDRMDGAWLVREPVDAAEVGRLYRLFVRAGLPRAFSPDRRYLVVLDASERI
                     VGGVSWSQLSPDAAQLDGLVVAPSVRGRGLSSALLDDLCARLASLGVRSLLAHFAFRN
                     LPLPGFRLDRRHGGLVRDLSAGLEVEAGLA"
     gene            15603..16022
                     /locus_tag="PLP50_03285"
     CDS             15603..16022
                     /locus_tag="PLP50_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPA50602.1"
                     /translation="MKQLQERLELRTPGRGLHEVTGAVAAVVARSGVATGLCVVFCPH
                     TSASLLLQENADPTARADLLTWLARLAPDGDPRYAHDAEGDDDMAAHLRSAVTRSSET
                     IPVSGGRLALGTWQGLFLAEHRTTPHRRSLVVHVFGE"
     gene            16024..18768
                     /locus_tag="PLP50_03290"
     CDS             16024..18768
                     /locus_tag="PLP50_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPA50603.1"
                     /translation="MRGARSARALPAALAALAFLALPAADASSASLAPVSGSSSLALA
                     PGFVIAASYRSPADGAERGYAIRVPRGWDGTNLLPAILFLHGRGGTVRQFQRTPYFDA
                     ADARGVVLVFWEGRIALGGAEVPSTHYVDGADGVPDETDVLACLDDVLSRAPVDAERV
                     TLAGFSQGGRGALGIGLLNPSRFAAVVDAAGPTDAFQGQLWSPAFPDYLAAAGGPPSA
                     GGEVLARWHELSPRFLLPNARNLFVAVLHGQVDDVVPDSAAYFPYRNSHHVTVTPGFS
                     DARGRTPTLAELHAEDEGGYAFSTDFPDDVGHDQVRLLTASALFEAALGKVRASRPER
                     VVGVSFGARERTFHWARLGRRIPPDGTRLLVDARSDLPENRVDLRAEGPARVRLDLPA
                     AGLDAARALSVRVDAVGAIDLALDGPFPPAVAATLDGRPVPVERTAEGVALPGLAPAA
                     PGALLVVAPAPAGPVAEGDRLVPALVRTEGANGARYDTVVTLANLSDGPLSLEALLLD
                     GASAPVPLLLPAGTSRAVPAGTLGAASAAFAAPLRLRVLSGEGDALAASARVFSASDD
                     GTYGLSFPVLTAGESVLSAGETALLFGPRDPASERLNVSLFAPFEASTAEVAVFDGRG
                     VAVRTRPVALAALARVQLDDLLRGEAPGAHVRVSVFSGRLQAYGTAVSNGPTNDPWRV
                     PALPLAAAAKAWTVPAVASAEGRNGAYFRSDLFVFAPEGASLDATLLPRDGSPHEKAG
                     LLVPGGAPHVVADLLASLFPSKAPGAGALLLSSTSPFLPLAVTRSEPPTGPSSQDLPC
                     VPEGGEVVPGRPVAFAGVDESTSARSNLVLVAPATAARVRLRLLAPSGPLGEHVVDLP
                     AGRVVQLDSVASRFTDDPVEGGTLLVSCESGAVVASVARIDAATNDPAGLAPLPVVVR
                     "
     gene            complement(18772..19542)
                     /locus_tag="PLP50_03295"
     CDS             complement(18772..19542)
                     /locus_tag="PLP50_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA50604.1"
                     /translation="MRAAVSVVFIVKDEADRLAPSLASVSWADEVVVADTGSTDGTQE
                     LARAAGARVETVPWEGYVATRNRAIGLAANDWVLVLDADEAVTAELAAEIREAVADPK
                     GADAFRMPRLSHLGARPIRHGVWWPDRKLRLGRRSRGLRAAGGHVHEWIEVDGEVRDL
                     SRPLLHHPYRDLADAVRKNVLYARLSALERFERGGRGSVLLLVLRPPLEFLRSYVLKR
                     GFLDGRAGVAVALLHAWYYLLRAGFLLEATEKAKLERA"
     gene            19564..20595
                     /gene="waaF"
                     /locus_tag="PLP50_03300"
     CDS             19564..20595
                     /gene="waaF"
                     /locus_tag="PLP50_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPA50605.1"
                     /translation="MRLLLRATNWVGDVVISLPALRALRAHHRGDHIAVLARPWVAEL
                     YRLLPEVDELLVEDPKGRHAGSEGRRLLSDELREKRFDRAILLPRSFTTAWTVYRAGI
                     PERWGYRGELRSPLLTHPVPFSPRAGEHELHRHLRLVEATGVPGPAIPDATLAVPDSV
                     RRGARAKLAEAGLDGGPFAAAHVASFAHEAKRWPEERFAALFAHLHEERGLPVVLLGS
                     AAEAAMNARVAALAAPAHVVDLAGRTSLADVLGLVAEAELFVGNDSGLAHLSNAAGTP
                     TTVAFGPTDPDATRPWDGPRPDGKPVSLRIARNRVPCAPCRFKRCPLDHACMTGLDLG
                     LDQGSGLVL"
     gene            complement(20643..21191)
                     /locus_tag="PLP50_03305"
     CDS             complement(20643..21191)
                     /locus_tag="PLP50_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPA50606.1"
                     /translation="MIDAHLTALAETALAARGAMKPAIEAAAAAVAAAVRAGNKTLAF
                     GNGGSATQAQHFAAELVVRYKGDRPGLPAVALVCDGAILTACANDYSYDYVFARQVEA
                     LGRPGDVALALSTSGTSPSVVKALEAARARGIVTILVTGTRGAAEAAKWDHGLVVPSA
                     ETAHVQEITLAVLHLLCAPLDA"
     gene            complement(21188..22627)
                     /gene="rfaE2"
                     /locus_tag="PLP50_03310"
     CDS             complement(21188..22627)
                     /gene="rfaE2"
                     /locus_tag="PLP50_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPA50607.1"
                     /translation="MAAAAPSVKAVVVGDLMLDETWLGDVSRVAPEAPVPLLALSSMC
                     RRAGGAGNVVENLAALGARTVALGAVGPEEEGAWIARHLGSLPGGEGRVVVDPLRTTP
                     VKRRMVADGRQLLRVDQELAAVLSEGAEEELIGAALAALESADVLLLSDYAKGTLTPR
                     LMETLLAEARRRGIPALVDPKGRDYGRYRGATCVTPNRKELETVTGESARDDAAVARL
                     ATRLRDDLGLDAILVKLSEEGMLLLPKGAAPQRIAARAREVFDVTGAGDTVLATLGIA
                     LGAGLPLGDAAQVANRAAGVVVGRAGTASVRWADLLDGLTASPAEKLLDRGHVAAVAQ
                     MLRRAHRRVVFTNGCFDLLHPGHVTLLGAAKRLGDVLVVGLNSDASVKRLKGPERPIL
                     SETDRAQVLGGLDAVDFVVLFEEDTPEALIHEIRPQVLVKGGDYTEESVVGAPFVRSY
                     GGEVALVPLVPGRSTTSMVTKVSKMKEAP"
     assembly_gap    22628..22727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22803..23843)
                     /locus_tag="PLP50_03315"
     CDS             complement(22803..23843)
                     /locus_tag="PLP50_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50608.1"
                     /translation="MTTTKRRRPRETVTTWPIATRSFKVKVDDFAAKPSTSGAFADFW
                     NGLPRILAAESLRKVVAAIHAARRKGKPVVVAFGAHVLKTGLGPILVDLVERGFVTAL
                     ATNGASGIHDFEIAFHGKTSEDVGPVLETGEFGMSRETGVGVFRALRAGRKNGVGYGR
                     ALGEGILRARLPFRHLSLFAAAAKADVPLTVHVALGTDTLVQHPGMDGALVGEMSIRD
                     FTTIRRVIAGLGDGGVWINAGSAVIMPEVFLKALSMARNLGHDVRNFTTVDLDMNRHY
                     RPHENVLKRPVLSGGASYAITGHHELILPLLAQALVLGLEDAGTTPSTLCLGEAHRMG
                     SPRTSSARGKRS"
     gene            complement(23840..24436)
                     /locus_tag="PLP50_03320"
     CDS             complement(23840..24436)
                     /locus_tag="PLP50_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPA50609.1"
                     /translation="MRRRPLLDALFLDRDGTLIAERGFLADPKGVRLAPGAAALLARF
                     AAEGTRLFVVTNQSGVARGLTRWDEVNAVNAEVERRLAARGVPIAGWLVCPHHPEGRV
                     KEYAVACRCRKPGTLLHRRALRRHGLAAERSAVVGDKWDDVGAGLALGAIAVHLLSGH
                     GREHRARVREKGSGRVILAGGLADGLRRLRAAAEGKQG"
     gene            complement(24433..24960)
                     /locus_tag="PLP50_03325"
     CDS             complement(24433..24960)
                     /locus_tag="PLP50_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA50610.1"
                     /translation="MAGPNVSANPRSAAAAVLAAALLVGAVSASAEPVKHLLPRDYAA
                     RVVAGKKGRVLVVNFWATWCEPCREEMPDLVAAARKFPSRDLAVTLVSLDSQKTGPTQ
                     VPKFLKEQKVPFVCWLAKSRDPQDFIDTVDRTWDGSLPYTLIYGRDGKIAARLSGLQT
                     EASFTEAIRRAVGAK"
     gene            complement(24963..25562)
                     /locus_tag="PLP50_03330"
     CDS             complement(24963..25562)
                     /locus_tag="PLP50_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA50611.1"
                     /translation="MKTPRLALALLGTTLALACTAPAEPPAAPKIGAVAPDFTLPPAA
                     GGEAVALKDLLGKSKAVVVIFDATKCPYAKGYKERVAAMGKEYAAKGITFVTINSNKT
                     EPAAEVADDAKKHGFAFPVLKDEGNRVADLYGAQKTPEIYVLDPKGTLLYRGRIDETH
                     DDPKNVKSPDLINALEAILAGKPVPAAETKAFGCTIKRV"
     gene            complement(25559..27781)
                     /gene="purL"
                     /locus_tag="PLP50_03335"
     CDS             complement(25559..27781)
                     /gene="purL"
                     /locus_tag="PLP50_03335"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPA50612.1"
                     /translation="MGASAPTITPALLAEHKLTSDEYERILAALGREPNLVELGIFSA
                     LWSEHCSYKSSKVFLRDLPTKGPRVVQGPGENAGAVDIGEGLAVVFKMESHNHPSFIE
                     PYQGAATGVGGILRDVFTMGARPIAVLDPLFFGDPSAPRMHGLIDGVVRGVGGYGNCI
                     GIPTVGGSTFFHPSYDGNILVNVMAVGLVRHERIFKAKASGVGNPVLYAGSKTGRDGI
                     HGASMASDVFDDEKSQRRPTVQVGDPFTEKLLLEAVLEVLEGDAVVAIQDMGAAGLTS
                     SSFEMCARGDVGMRLDLDRVPMRESGMTPYELMLSESQERMLLVTKKGREREVIEVFE
                     KWGLDVAEIGEVTAEPRMVLRWHGEVAADLPVAPLVDDAPVYRRPWTLAEPLPPLEGA
                     IDLSGAPDETAALLALLASPNLCSKTWIWRQYDRSVRTNTVVGPPGDAAVLRVKGTKK
                     GLALTADVNPRYCFADPMRGAAHAVAEAALNVACTGARPLAITDCLNFGNPERPEIMG
                     QLAAAVKGLSLACRALETPVVSGNVSLYNETDGESILPTPTVGMVGLLDDVARAVPHA
                     FRAEHDVVALLGETRDELGRSEYLATVLGRDEGPCPLLDLLSVRSMVDLLVALAADGL
                     LSSAHDVSEGGLAVALAESAIPNGLGADLNVETALLPAIYLFSQSTPRVVVSFPPGHE
                     RAVLEAARRHGVPTAFLGRVSRGSLRVAVSGNVVLDVPVSTLTRVSEEAFGKMMEVTE
                     "
     gene            27837..29024
                     /locus_tag="PLP50_03340"
     CDS             27837..29024
                     /locus_tag="PLP50_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPA50613.1"
                     /translation="MFSRRTPAPGAENRLTQALAAHPRPVLDLTATNPTAAGLPLDAG
                     PALALLADPRGARYDPDPRGLLSAREAVAGHYAGRGVVADPARLLLTASSSEAYGWLF
                     KLLAEPGDAVLVPAPSYPLLDALAALEGVELVRYRLPAEDRWTVHASLVEEAADRAEA
                     AGRRVAAAVLVNPNNPTGTSIAAEELDTLSALAARRGFALVSDEVFLDYRFEDRPGDV
                     RVAAAEPGEALVFSLGGLSKSAALPQLKLGWVLANGPAGPLAEALERLAWIADSYLSV
                     GTPVQLALPGLLSFGEGAARVLRARLLGNHATLAGALAPLAGVTVFPLAAGWSGVVRL
                     PAVEREEELVLRLLGEEDLVVHPGYFFDFPNEAYLVVSLLPGEETFREGAARLARAID
                     RPA"
     gene            complement(29035..30090)
                     /locus_tag="PLP50_03345"
     CDS             complement(29035..30090)
                     /locus_tag="PLP50_03345"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HPA50614.1"
                     /translation="MRREPTREATIGNPGRVIALVILALGATSATGAAGATTAETWLH
                     PVLPESVVKAAPAARRGGRAARPAPPARPAPFPTAVSGDTSFVEVTTGESYPEPGSAV
                     PPERIERSFARLGEGRPMPLTGARVDGPLTRLEATWLGQGLATLGLLLLPEGRTVEAA
                     AFETFLREAGAREALALRARKKETKKPARVVTIESARAFAGVVAPPRAKRTADPAAGR
                     DEALGLPLELVAGGSPLTLRAGDVLAATLLLDGRPLAGALVRAFAPGANAALEAETDA
                     HGTASIPLEREGRLLLSAAAVRRTEKADRTRGEIWKKADWEVRRTTLELLVLPKAPAK
                     AAPAPKGKRPPAKKKPR"
     gene            30172..36069
                     /locus_tag="PLP50_03350"
     CDS             30172..36069
                     /locus_tag="PLP50_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPA50615.1"
                     /translation="MARGEKKSRSRARRVLLGVVTLLVVLAAATIALLKAPPVGRRIV
                     DAALRSAGAASGLAISARERSLDALGGRLRLGGLVVGLPGEPPFLSVDAAEAELLVGE
                     AIQGRIRLRFVTLEGVRLDFGAPWPESEGPPATNLPFLSAAQIERFRIEGATVLSGPL
                     PEALRAFALSASASDVRLDGDLRNGTLRLRGVTPRIVVERPGGLRLEAKGDVALTASA
                     DGELVLEALHVDGEGLALSAFGGGGLSPEASLGLRAEATLDPARLAPEMGATGSLRIS
                     AKMEGRRAAPTVEATLEGRDLATEELAIALATARVRFADETLLLESARADLRDGGRVE
                     GEGRYLFATGDGSWDLRATGLPDTLLARYADPATRERLGIAGATLDAVATVRHGRGEP
                     QPLEVDATATLSCDGGTLAEASAGLVARGDAKVDVAATFLPDSPGKRTAAGRVRAASL
                     AGLASGRIEAGRVALDLPDAAEAAAELRALFPSLVPEPPEGLDLAGPLRLDAGVSGPL
                     RAPRAAIAGTFRPARGGELALDASADAARGAAAGRLTAAGLDVATFHPGATGLASADV
                     AFTLSRKGRHADVVLDAADLCLAEETPRVDALHATLALDGPELSILHLAATSAAAPSP
                     AGPSPARVDASGRLSLDAPFDDADLETLLFAAGLPAEADAVVRDGVLFLDVPSAGRPG
                     LVGTLAARVPLGSLRESPALAGAIPAGLPEGPIEVTLEAPGLDACALGPLLPSGTEAL
                     AARGDMRLFATFGLADPLAGTAAIEIEGLTLESPAGPVRLRETARLSLAGGELALQPV
                     TVDGERTSFTVAAEAELLPGARLGGPLESLVEHLSATARGRADAALLSPFLQGGIAKG
                     ELTLDASASGPPRALAGHVLVDGRGARFTWPLAYPTELGDPVLEAELTPGQVELTRGE
                     ALLNGGPLRLEGGWYSGVGATATARFSDVRYRLAYGLASVLSGELTFDQLEDERRLSG
                     TVVLERGLLERDVDLDREVLALVLAPPESPGTEASFLDTLALDLGITTASGVRIRNNV
                     ADLTASWSRIDVSGAASRPVVRGRIDVESGGLVFAYGQTFRIDRGVVTYSGDPQSDPR
                     LDFLTTSSLEDPSIAAAGGGSDLFASARRDASGGASSEDAAAALAEGLAGYYGNRLAG
                     SLGAALGRVSLGVRPLLLLGETDAAARLTLSRDFSPNVSLAVGIDLKNAQRQTWVVDV
                     HGLRRLPPLSVQAFTEDYGRFGGTLQQRIELGGTRDGAGDDVPLVGAMRATPPAGVSR
                     RSLVSALGLRKGEPAGKDALFEAEIDAEAFLRGKGWPDAQVSLRAVPSRKTGRVDVEA
                     EIDAGPRAELSFAGDRLPAAVRREVASLYRTGLLEPASLGDMRRAAERALRARGHLAP
                     AIEVEATGPEEERRVVIRVVAGRKAAIGELRFEGVDPREAVLLGRRFATPLERTELAA
                     GLPSADARLLEGLRALGYPYAALGERELLDGGRLVVRVERGEPSLVDSVEIRGVSPEE
                     RERFARLVRLAPGDVADSERTAFSALAIEEALRAEGFAAARVRATLSPATPEVPPRLA
                     LAYAVERGPARRIGNVRLEGLSRTSPAWAKSVAALSPGDVFRRSDLDAARAGLFSLGL
                     FRSVRGDLTTGPDGRVDVVLAAEELPPVTLAYGVRWENERGFSAVVDASDRNLFGRGI
                     VAGVRGLYDPEDRALRLFAGLPEQVLGAGIDLWVERRRSFREGLFYGQRSDAVEASLQ
                     LSRSLGKSLSARLYGRFKETRYFEDDIFFPIDVTIRLPYLGVQLVHDTREDPMLGTNG
                     VLATLDLQSSGSWLGSSFAFGRAYGQLNLYRPVLALGSGKVVWAQSVRAGFARAFEGQ
                     ELIPDVRFYAGGSYSVRGYGTESLGPREDLGGTLYVTGGSTLLVVNEEIRVPLHPRLL
                     GVGFLDAGQVWESSRDFGTGLATSVGLGLRALTPLGVLRLDGAVPLKRREGDPAFRIT
                     FGFGNVF"
     gene            36246..36848
                     /locus_tag="PLP50_03355"
     CDS             36246..36848
                     /locus_tag="PLP50_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPA50616.1"
                     /translation="MKKNLLSAALATVLGLAGAPALAETWTADTSHSTVGFSVRHMMV
                     SNVKGAFNKFTATVEGSPADPASAKISATIEVASVDTREPKRDEHLRSADFFDAAKHP
                     QMTFVSTKVEKVSATKAKVTGNLTLRGVTKPVTLDVEYTAPVKSPWGQTVIGATATGK
                     IDRQDYGVSFSKALETGGVLVGDEVTITLELELVKQEAKK"
     gene            complement(36922..37713)
                     /locus_tag="PLP50_03360"
     CDS             complement(36922..37713)
                     /locus_tag="PLP50_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50617.1"
                     /translation="MTRSFLALTLAAPFLLAAPPAGAQTAPAPTAAPAPPTPLEAARG
                     AERKLDSWMTAEARTALSPVAAQAASNVDVAIALGRLLEQEKKYAESADVLQKAVALA
                     PADPRPSHWLGETLLRARKTAEADAAFRKAAELATKAAEARPTDAAAFLAKGAALSRL
                     RRFDEAMTALGKARELDGGSAATLYQMGSTRAFQQKWGDAVDLLTQTLAKDPGMALAY
                     YYRGLAQEKLGRKDQMVLDLDRFVKIAASAPEAERAKALLAAASR"
     gene            complement(37739..38827)
                     /gene="rlmN"
                     /locus_tag="PLP50_03365"
     CDS             complement(37739..38827)
                     /gene="rlmN"
                     /locus_tag="PLP50_03365"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPA50618.1"
                     /translation="MKTPLLGLTLPELAQALAPLARERFRAAQVQRWMTERRARSFDE
                     MTDLPAPLRAALGERFVVGLPEVEARTPSDDGAEKFLFRLADGAKVEAVFLPASRRRN
                     SGTTERVTVCVSSQAGCALTCAFCVTGRMGAGRNLSAGEILGQYLVVAREKGFGPRAA
                     NVVFMGMGEPLLNVANVSRALDLLEAEVSPRRTTVSTAGIVPGIRELAGRERRPNLAV
                     SLTAAEDALRTRLMPINAQWPVRELFEALEEWPLEPGRRVTLEVVLIADVNDSPEAAR
                     ALARLARRVPSKVNLIPLNESPEWLPGLARPADEKVNAFGALLAASGVDVTVRWSKGL
                     GAAAACGQLKGRETLGRLPRVPAPSVRS"
     gene            complement(38881..39261)
                     /locus_tag="PLP50_03370"
     CDS             complement(38881..39261)
                     /locus_tag="PLP50_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50619.1"
                     /translation="MALDEKTRAALRQLGKALNEAVSSSAEVHGILQSLREEGHEPYL
                     VLDATVALDARGRRARAALPSIRRGGPAGREEAEGAAFKIDVKDLHFLRSLGIDPTRA
                     PRAPRGRRRTGTAVSLRTTVSKRV"
     gene            complement(39295..40170)
                     /locus_tag="PLP50_03375"
     CDS             complement(39295..40170)
                     /locus_tag="PLP50_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HPA50620.1"
                     /translation="MTESAALASAHRENRALTHKAGANFSVGFRFLPKERRHAVYAAY
                     AWCRVADDAVDEGDPAGAPARLDAWEEELDAVYGGTPTSAAGIALAGSLPRFPIPKTA
                     FQGLLAGCRQDLVKTRYATFDELLGYCDLVASTISTISLAIFGGLGNAEAEARGRDLA
                     TALQLTNVVRDVGEDVGRGRLYLPLEDLARFGVTEADVVSLRATPAAAELVRFEARRA
                     LSYFRRAEPVKELVDRECRFAVTMMGGIYADVARAVLARPLDTLRRRISLSTPGKVGA
                     VLARLADRRFDRGED"
     gene            complement(40167..41303)
                     /locus_tag="PLP50_03380"
     CDS             complement(40167..41303)
                     /locus_tag="PLP50_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPA50621.1"
                     /translation="MPDLPHLPIAGAGPAGLTAATLLARAGRRVVVHERNATVGARFV
                     GDLQVIEDASDPRETVPALFSRLGLPAPAWVPATTAEFFDAKLRRFAVSSREPYAWFV
                     TRGPGEGTLDTTLLAGALAAGAEVRFRSRLEAKDADLVATGPQTPDGLARETAFESDG
                     VTRVRVLFDPSVAPGGYAYLFTVGGRGTFGCAIVRDLGGIDRYFERAKAKLLSVESVP
                     MEGARDAYSFMNFALAPEASREGRPAVGEAGAFQDYLFGLGMRYAISSGALAAEALLA
                     GGDFAALARARFARRQRVSVANRFLYEAGGAFGLSLFARLAAHADFRSVLAGFYRPGA
                     LRLALAPIVTALWRNRGRCAHRLPEHWCRKREREIREVALGRIG"
     gene            41498..44134
                     /locus_tag="PLP50_03385"
     CDS             41498..44134
                     /locus_tag="PLP50_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPA50622.1"
                     /translation="MRFDSLRPRLEPFLLRVERPGRYLGLERNVVRKELSAAELTLCL
                     AFPDTYEIGMSHTGTKVLYELVNRRDGWACERAYAPWVDLEEVMRRERIPLFSIESCA
                     PVADFDVLGFSLQSELNYTNVPNMLDLAGVPVLAADRGDADPVVVGGGPCVANPEPVA
                     DFFDAFLLGDAEEALAVFLEAVRATRGLPRRERLLAFAACPGIYVPSLYDVSYDGDRV
                     VAYTPNAPGVPERAKRVWVERLDPSVYPERPMVPSVDIVQDRLGLEIMRGCTQGCRFC
                     QAGYWYRPVRELDPADVARMTKAFIDEAGWSEVGLLSLSSADYSQIEPLVACLAPELA
                     KTKVSISLPSLRAEAFSVALADAVSEVRKSGFTFAPETGSDRLRRVINKTFTNDDMVK
                     AAGVAFERGWDLIKVYTMIGLPSETTADLDELVTLVANILAEGRKKGVRATVHVSIGS
                     YVPKSFTPFQWSAFDGVERLTEKLAYLKERFRPVRGAKLKWHEPKEAEIEAMLSLGDR
                     RMSGALLEVWKRGGKFDGWSEHFSWPRWTEALDAAGIPKARHLREKDLKETLPWDVVD
                     ASIRKPFLVVEWKKALQEMATDDCKWGRCTACGVPGNGEDTVLANGMPAAFSFALPTL
                     GAAEAGAPPAPVPGDAGDGGRAAVEISALPSASGVASGVADARDEGRGAAYADKAKGA
                     AYRMKATPDLLPLRERRTSRGGASESAAIARSWRVSFEKLGDARYLSHRNVMDVLERA
                     LRAARVPVRYTEGFNPHIRLSMGPALPLGSESRHELFDLDVVEPLTLEHLAAVNDRLP
                     PGMRLLGWTELPPGAPSLGKAATEGVYRFTLPNGEVIVERLRLAGEGATTPKRHIEKT
                     WGLAAEDQHVIRVVREETVLTR"
     gene            44158..44532
                     /locus_tag="PLP50_03390"
     CDS             44158..44532
                     /locus_tag="PLP50_03390"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPA50623.1"
                     /translation="MPFTIRCHATFEAAHHLRDYVGGPEPVHGHSWKVEVALGTDGLG
                     SYDLSVDFVPTERLVRELAARLHDRDINTVPPFDVKNPTAENVALWFADEVRKAGVLS
                     DGVRLEEVMVWEGPRNSVTYRP"
     gene            44800..46329
                     /locus_tag="PLP50_03395"
     CDS             44800..46329
                     /locus_tag="PLP50_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50624.1"
                     /translation="MSCRSIGALFGLLLLVAPRISADEVPLRNWTVPSSPTRLSALGD
                     IGNPGVFVAVTPCRVVDTRLGMGFSGAYGPPALTAAPRTFNIVASGCTGLPANVSAFS
                     LNFAVTNTAGPGHIVAWPAGGTMPNVSTLNYLGAQTLANAAIVPAGSGTSISVVAAVS
                     ATDLIIDINGYFTNYPNTNKQLFTSGSFSAAAAILGVNDSNAIGSHGVGGYAGGTGVV
                     HGVQGEIGSGAADLSAGVHGIGGAGTARTFGVYGESATNAFDAAGVLGRSTFGTSPII
                     NSIANTAGVRGEGAARGTVGVAGPSGVGVRGYSVDASGILQTFGSLGQGNSYGVYANG
                     DLGATGTKTFVEPHPTDASKVIKFVSLEGPEAGTYFRGTASTRGGVCVIEVPEPFRMV
                     TDEEGLTVQLTTVGSGAHAWVESESLERIVVRSSADTKLHYLVQGVRKAYRDFQPVVP
                     GDEFMPRSPEDTLPLWLSEEGRRRLVSNGTFHADGTVNMETAERAGWAQRWREAERVP
                     E"
     gene            complement(46408..48282)
                     /locus_tag="PLP50_03400"
     CDS             complement(46408..48282)
                     /locus_tag="PLP50_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50625.1"
                     /translation="MMREAFYSTVRGAADAAWKGFQWVNRKVPARSFHPAWSPEPLPK
                     SHERTKPPLGFPRRTDSLCPVCTREVREAIVKGQEDLKALIDGHPGEIPADIVQRGQE
                     IWMVKTCPKHGTFEDLMSNDVDFYRRLEGNFFGRDVRIGKDALHDHGSSSMRYGRGAV
                     LTVDLTNRCNMMCNPCFMDANQVGYVHELTWDDVKQILDNAASIQPRRQLSVQFSGGE
                     PTLSPHFLEAIRYSKEIGYFAVQCASNGVRFAQEPGFAKAAAEAGLRLVYLQFDGVGN
                     EHNQHRGVSNLYDVKLRALENLKAAGVDVTLVITIVNSINNDQVGDIIRFAIKNIDKI
                     NALSFQPVSFTGRDEEIDDETRKRQRYTLSHLANDVKGQAGLGEPMRDWFPLSASGPF
                     SDLRDLLGGLEAEWGSLKCGCHPNCGIATMVLVHEKTGDAVPVTQLLDVDRLLRDLTV
                     ITDAARGRFLTVLQAVLATARNIRPAGVPRNLGVLETLRIMDGHTGKSMGIAKSGRYP
                     WRVLLVAGMWFQDLFNYDFRRTEMCIIPYGTQLGEISFCAYNTGAGWRQIIEEMHQTA
                     TLADWYRDKGRHPIYAGGKSVPLPTYRPRPSVAPEELPEAAKAGVDMALPSFGTAAGR
                     "
     gene            complement(48379..49503)
                     /locus_tag="PLP50_03405"
     CDS             complement(48379..49503)
                     /locus_tag="PLP50_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA50626.1"
                     /translation="MTRTLSAADAVVCGTGLAAAAVALELARRDLDVVLLGSGRFGRP
                     RGPGAPVAAQAFVEEAVPEARARLAHLARDLFPDWLSDLEEETGVPCELDERGALTVA
                     FDEPEEVLLDRALDAQRRRGVPFEVLEPEEALRREPALTPELRAAFSFPRDGVARARR
                     VGKALVLAARSAGVRLIDGTGARVVARTGGRVSGVETPAGFLPSAAVVLAEGEAVERG
                     EAGLPPFPLEAASRPWLRLDASSDPDRPGRLLVSSGVRLVPRRDGTLVALGRPGRDGR
                     TRRCSASRLAILLEALRQLVPASMAWEAGESGAAPELRTPDGLPLAGETDVAGLFLAT
                     AWGSDELAFSPAAARFVADLVTRHEPPAPAAPFAPARFGL"
     gene            complement(49500..50099)
                     /locus_tag="PLP50_03410"
     CDS             complement(49500..50099)
                     /locus_tag="PLP50_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50627.1"
                     /translation="MKIAESFLSLVRRASTDLDVERIRDGVLELSRRHPGLSTRRKAA
                     LMVESTARRAALVGAAASVPPGWAAVAATAPELTTLIVLQSRMIVGLHLLYGADLDPE
                     ERALEVVAGLASGAGLSVGRRLTVGVAESLATRLAVRAAGRPLAHLIPVAGIAASAAL
                     NYIAVRAVGRAVVARVERRWGPPEIAGRGPVLTAEGRVA"
     gene            50332..50898
                     /locus_tag="PLP50_03415"
     CDS             50332..50898
                     /locus_tag="PLP50_03415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPA50628.1"
                     /translation="MSSTPRDYAKTLAVLKTSQGDVTIRFFFDKAPGHVKNFIDLAAR
                     GFYDGTLFHRVIPGFMVQGGDPLTKDPAKSSLFGTGGNTDKAGKPVNVKAEFNDVSHK
                     RGVVSMARASDPNSASSQFFIVVQDSPFLDHQYTAFGEVVKGLEVLDKLVTESNSDTS
                     DPRSGGKPRAYQKVLKVELAAEAAPGNA"
     gene            50895..51827
                     /locus_tag="PLP50_03420"
     CDS             50895..51827
                     /locus_tag="PLP50_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPA50629.1"
                     /translation="MTSPGPAALEARVSAACSRFGLPESGPLQLLAGDVGQRRYVRLR
                     TWADATVVLVLYPEPASPAQERWRRIGEALGREGLRVPALLDDDPASGTALVEDLGDR
                     DLAAEIREADPGERGRLIEEAEELLAPLRAMSREAALLNPPFDAAFFVAELAHTRRWA
                     LESDGARALSPERSAAWEELAASLAREAADASRAGGPVPTHRDFHANNLMRTPEGALA
                     LIDFQDLRLGHPDYDPVSLRFERAGALVPSSGDDRSEAVLLQRAWKVLGTFEKMLALG
                     REVYRPHRDTALLTIRARTRPDGAWAPLLDFLPA"
     gene            51860..52069
                     /gene="tatA"
                     /locus_tag="PLP50_03425"
     CDS             51860..52069
                     /gene="tatA"
                     /locus_tag="PLP50_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPA50630.1"
                     /translation="MPNLGLPELLILLFIVVLLFGVGKLPQLGKGIGEGIRNFKQAVK
                     DGGQDSEKKEPPTGDGSGNGSSTPK"
     gene            52196..53107
                     /gene="htpX"
                     /locus_tag="PLP50_03430"
     CDS             52196..53107
                     /gene="htpX"
                     /locus_tag="PLP50_03430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPA50631.1"
                     /translation="MKIAKRVFLFLAVNVLVVATISVVLSLLGVRPYLNAYGLDLRAL
                     AVFCLVWGFGGAFISLALSRVMAKWGMGVKIVDPTTPDPGAQELVSTVHRLAQAAGLT
                     KMPQVGVYDSEEVNAFATGPTKNRALVAVSTGLLRRMRREEVEGVLGHEIAHVANGDM
                     VTMTLVQGVVNAFTMFLARVLAFFVAQFFRRGDEEGGGVSHLVYFIATLVFDIAFSIL
                     GSFVVAWFSRLREFRADAGGARIAGRDRMIGALEALQRTYGIVDPSDRQAAVQTMKIS
                     GHPKGLMRFLSTHPPLETRIARLRMLA"
     gene            complement(53281..53511)
                     /locus_tag="PLP50_03435"
     CDS             complement(53281..53511)
                     /locus_tag="PLP50_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA50632.1"
                     /translation="MLDGQLQRIIDELVSKGIPLETARKEFERKYVATAVTRASGNMG
                     QAAKSLGIHRNTLRAKISLLRLKPAPKRRDHA"
     gene            complement(53649..54518)
                     /locus_tag="PLP50_03440"
     CDS             complement(53649..54518)
                     /locus_tag="PLP50_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50633.1"
                     /translation="MKRVFVPAMVVLACAVISLSAAAASPSRLPEAAPVPMPQEAVEA
                     PAVIEQAPTERTSTSAPFPVGWEDDVYCAGWIGVDEEPVTGRIVAAEYEDSRQMFGVG
                     DVVYSDVGARAGLMAGQEFQVIRPAHEVYQVGSYTATIGRFYETPARARVVCVQDDSA
                     ILEITESCEPTYLGDLLIPFEPIPIPLVRASAPLTQCDPPSGKVTGRIVEVKDRATPV
                     GTDSVVFLDLGEDDGLYPGDFLTVFRKRNDSGTVRTLLGEAAVLWTKGKTCVAKITSM
                     VDSMQAGDFVELK"
     gene            complement(54621..55433)
                     /gene="bamD"
                     /locus_tag="PLP50_03445"
     CDS             complement(54621..55433)
                     /gene="bamD"
                     /locus_tag="PLP50_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPA50634.1"
                     /translation="MAHRTHAKTAASLVLGLAVLGGGACSSSDPSRVTRELLTMSKED
                     AYAQGDALVTKRKFEVGRQYLRFVAENYANDPLGKQAALRLADSFLEEKSPTGYVEAQ
                     ARFKDFRSRYPSHPRSDYALFRLAQVSDRQAEKPDRDQANTRLAAQSYSDLVVSYPSS
                     PYAFEARARYKAMLDLLAEHEFLVARFYYRRGAWQAARSRFEGLFAAFPEYSGMEKAL
                     YWAGQTERRLGRTEDARVLFDRLRRDHPESRFLRKVPRLPETPVAATASTGL"
     gene            complement(55448..56176)
                     /locus_tag="PLP50_03450"
     CDS             complement(55448..56176)
                     /locus_tag="PLP50_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPA50635.1"
                     /translation="MSGVLARILYALLLAGILGAAAWISFSRFVLGKAEEVPDLTGRS
                     VDEATALAAERGLRVVVDATQEGFDEEIPAHRVRGQSPAAGMAVKAGQDLRVFLSLGP
                     RVIQAPDLAGLTARTAALALAREGLVEGAVSAARMPGPQGVVAQGVVPGAPTEPETPV
                     DVLVNRGAPDVLYVMPDLIGRDFERVRLAFEARGFRLGGVKAQPYEGAAAGTILRQFP
                     LAGAPVGLRDTLSFVVASPEPQPG"
     gene            56260..57234
                     /locus_tag="PLP50_03455"
     CDS             56260..57234
                     /locus_tag="PLP50_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HPA50636.1"
                     /translation="MTRSRGPAATPELPLSRADLHVHTNASIFKYFKAANSHDSYNDP
                     EEVYRLAKARGMDFVTFSDHDTVEGCLRFQDRHPDLTDFFVSVEVETWFPKTGHRIHV
                     NVFDLTRQQHAVIDRKRKSIFDLVEYLRAEDLLFSANHLFQSYRMRQAPEVFFETMLS
                     LFDVFEVKNGSMAYQHNALVEDLLLVAKEKRGSLALVGGSDAHTYPPIASVFTMAPGK
                     TWKEFLASIRRGECLSWGSEMGFTNVLGDVYRSIGKHYREVTKLRNPDYTPREKAQHL
                     FFSIASLPIFATGFPAAVLSLNYVKQVALSKNLSRRFARTGLARPREG"
     gene            57231..>58018
                     /locus_tag="PLP50_03460"
     CDS             57231..>58018
                     /locus_tag="PLP50_03460"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPA50637.1"
                     /translation="MTRRPAGTGPLVGAHMSAAGGLVRAVERAVDAGCRTLQVFTKNS
                     NQWGGKAVVAGEAAAFRKAAADAGLAPLVSHSAYLINLASPDPLVRTRSVEALVDEIE
                     RCDAHGIPYLVLHPGSHGGDGEEVGLDRIARGLDEAAAKSPASGTTVLLETAAGQGAC
                     LGHDFAHLAGILARADSRPRLALCLDTCHVHAAGYDVVSKEGWRRTLDALDAACGLGL
                     VKVIHANDSKKERGCRVDRHERIGHGTIGAKGFANLMTEPAFEEV"
BASE COUNT         7508 a        21666 c        20722 g         8022 t
ORIGIN      
        1 gggggcgcgg ggtgggtcca tagggtgggt ggggggcgcg ctccgacccg aaggcgagga
       61 gcggggtggg cacacagggc ctcctatact cggaccatgt cccacgcccc cgcctccgca
      121 gcttcttcct ggctgaagca gtggcaagcg gccgggcccg ccctcgagga ggtgcggagg
      181 cgcgagctcc gcgagctccc cgacgacgag gcgctcgccg cggccgaggc cctcctctct
      241 ctggccgcct cgtctcccct gccggaggaa cgccggatct cctcgggcct cgttacccag
      301 caggcgctcc ttcaccgacg gcgcccgcga tgaacccgat cttcgcggcc gcccgcgaga
      361 tccaggactt ctgccggggc cggggctggc gctcctgcgt catcggcggg ctcgcagtcc
      421 agcgctgggg cgagccgagg ctcacccggg acgtggacgt gacgctcctg accggcttcg
      481 gcgccgaggc ctcctacgtc gacggcctcc tctcggcgtt ccgcggccgc ctctccgacg
      541 cgcgcgagtt cgccttgcga aaccgcgtcc tcctgctctc gagcagcacc ggcaccccga
      601 tcgacgtcgc gctcggcgcc atgccgttcg aggaggcggc cgtctcccga gccacgccct
      661 tccgaatggg ccccgacctc gaggtcctca cctgcggcgc ggaggacctc gtcgtcttca
      721 aggctttcgc gggccggccc cgggactggc tcgacatcga gggaatcgtc gcccggcagg
      781 ggacgcggct cgacgccccc ctgatctggc gcgagctcct ccctctcctc gagctcaagg
      841 acgatggcga gaccgagggc cggctccgcc ggcttctcgc gtcctccgcg accggagaca
      901 ccgggtagtc cgcgccgctg gcgccggtcc gggtccgccc gccgcgctca gccgcaggga
      961 ggccgttcct ccccctcgac gaccgggacg ggcgcaggga cggccttcca gatctcgcgg
     1021 gcgtagtcgc ggatcgagcg gtccgaggag aaacgcccca ttcgcgccgt gttcaggatc
     1081 gaggcgcgcg cccagcgctc cgggtcgcgc caggccgcct cgacctcgcc ctgcgcttcg
     1141 acgtaggcgc ggtagtcggc gaggacgagg aacgggtcgc tcgtgaggag gccgtggacg
     1201 agcgggcgga ggagcccccg gtctccccgc gtgaactcgc ccgagacgag ggcgtcgacg
     1261 gcctcgcgga gctcggagtc ggcctcgtag cgctcgccgg ggcggtagcc gtcgcgccag
     1321 gtccgctcga cctcctcgac cgtcaggccg aagaggaaga agctctcggc gccgacggcc
     1381 tcgcggatct cgacgttcgc gccgtcgagc gtcccgatcg tgagcgcgcc gttcagggcg
     1441 agcttcatgt tgccggtgcc ggaggcctcc ttgccggccg tggaaatctg ctcggagagg
     1501 tcggagcccg cgaagatcgg gaggctgctc ttgacgttgt agtcgggcca gaagacgacg
     1561 gcgaggcgcc ccttcatcgc cgggtcgccg ttgacgacct cggcgacgga gtggatcgcc
     1621 ttgatcatca gcttcgccgt cgcgtagccg ggcgccgcct tccccgcgaa gaggacggtc
     1681 cggggcggag aggcgagccc cgggtcgcgc ttcagccgcc ggtagagcgt caggacgtgg
     1741 aggaggttca ggtgctggcg cttgtactcg tggatccgct tcacctggac gtcccagagg
     1801 ctcgccgggt cggcggagac gccggccctc tgccgcgcgg cctccgcgag ggcggcccgg
     1861 ttcgccgcct tcacgcgccg ccaggcctca cggaagcccg cgtcgtcggc gagcggctcg
     1921 aggcggcgga gctcgtcgag gtcgcggacc cacccgtcgc cgatcgcctc ggtgacgagg
     1981 cgcgtcagac cggggttcgc gagcgcgacg aagcgccgcg gggtgacgcc gttcgtcacg
     2041 ttcgtgaact tctccggcca gatcgccgcg aagtcgcgca tcacgtcgcg ccggaggagc
     2101 tcggagtgga gcgcggcgac gccgttgacg tgatggctcc cgacggccgc gaggtgcgcc
     2161 atccggatcg acctcggccc gccctcgtcg acgagggaga gccgcgcggc gagcccctcg
     2221 tcgcccggga accgggcacg tagctcgtcg aggaagcggc ggttgatctc gtagacgatc
     2281 tcgaggtgtc gcgggagcgt cttcccgaag agcgcgacgg gccacttctc gagcgcctcc
     2341 ggcaggagcg tgtggttcgt gtaggcgaag gtcgcgcggg tgatcgacca cgcctcgtcc
     2401 caccccattc cgtgctcgtc gacgaggagg cgcatcagct cggcgacggc gatcgccggg
     2461 tgcgtgtcgt tcagctggac ggcccatcgg tcggcgaggt ccgccaggtc gggcgaggcc
     2521 tggaggtgga tccggatcat gtcctgcagc gagcaggaga cgaagaagtg ctgctgcgag
     2581 aggcggagcg ccttccccgc ggccggctcg tcgttcgggt agaggacctt cgagatcgtc
     2641 tcggagccga ccttctcctc gacggcgccg tagtagtcgc cgacgttgaa cgcggcgaag
     2701 tcgaacgact cggccgcctc ggccttgaac aggcggagga ggttcacgtt gccgacccgg
     2761 tagcccggga tcggcgtgtc gtaggcgacg cccttcacga cctgcgccgg gacccacctc
     2821 acgcggtagc gccccgactc gtcggtgaag gcctccgtcc gcccgccgag cttcacctcg
     2881 aaggcgatct cggggcgcgg gacctcccac gggttgccgt actggagcca cttgtcggtc
     2941 ttctcgacct gccagccgtc ccggatctcc tggtcgaaga tgccgaactc gtaccggatc
     3001 ccgtagccga tcgccgggat ccggagcgtc gccagcgagt ccatgaagca ggccgcgagg
     3061 cgcccgagcc cgccgttgcc gagccctggc tcctcctcct gcgcgaggac ctcctcgaag
     3121 ccccagccca tctcctccac ggcctgccgg acctcgtcga ggacgccgag ggccacgagg
     3181 gcgttgccga ggtgcgggcc gaggaggaac tcggcggaga ggtagcagac ggagcggacc
     3241 ctctgccggt agtagtgctc tcccgtccgc atccagcgcc gcacgaggcg gtcgcggacg
     3301 gcgtgggcga cggcgaggta ccggtcgttc cgcgtcgcca tcgccgggaa gcgcccgacg
     3361 gccgtgacga ggctgtcctg gatcgcccgc ttccacccct cgacccgcgc gcgggaaacg
     3421 ctctccgcct cgtcgccggg tccggggacc ctctgctccg tcatggctcg tcctccgtcg
     3481 cctggccgcg cgcggctccg cggcgcgacg gccggctcag tttcctggag acgtttctcc
     3541 gcatgctatc gcagcggacc gctctccccg cgccccacga ccagcaagcc cgcgcgaagc
     3601 gcgcaccgcg aagggcggtg ggtggggggc gcggggtggg tccacagggg gggcgcgggg
     3661 tgggtccacg ggatcagcgc tcgatgccat acctcttcag cttgtcgtag agcgtcgagc
     3721 ggtcgatgcc gaggacggcc gcggtctcct tcacgttccc gtcgttccgc ttcagcgtcg
     3781 cggcgatgac cgtcttctcg acctcggaga ggggcaggtg cgtcgggacg ctccagccgg
     3841 gcgcggcctc ggagacgggg cggaggaacg cgaagtcgtc ctcggtcagg acacgcccct
     3901 tcgcggtgac gagcgcgcgc tcgacggcgt tctcgagctc gcggacgttc cccggccagc
     3961 ggtgggcgag gagcgccttg agggcgccct cggagaggtc ttccgcctcc ttgccgagct
     4021 cgtgggagag gcgctcgagg aaggagcggg cgaggagcgg gacgtcctcg ggccgctcgc
     4081 ggagcggcgg gaggtgaatc gcgacgacgt tcaagcggta gaagaggtcg tcgcggaagc
     4141 gcccctgccg gacctcttcg gcgaggtcgg cgttcgtggc ggcgacgacg cgcacgtcga
     4201 ccgcgacctc cgcgctcccg ccgacgcgga agaagcgccg ctcctggagg gcgcggagga
     4261 ggtcgacctg aaccttcgcc gagatgtcgc cgatctcgtc gaggaagatc gtcccccctt
     4321 cggcgagctc gaacttcccc ttccgcctct cgacggcgcc ggtgaaggcc cccttctcgt
     4381 ggccgaagag ctccgactcg aggagcgtct cggtgagggc ggcgcaggag acgccgacga
     4441 acggcccctc ggccctctcg cccgagaagt ggagggcccg cgcgacgagc tccttgccgg
     4501 tgccgctctc gccggtgatc aggacggtgc tcttcaggct cgcgacgtcc ctcacgaggc
     4561 cgaagatctc ctgcatccgg gggttcttcg agaggaggtc gtggaagcgc cagcgccccg
     4621 tgaggctccg ccggaggatc tcgttctccc gctcgaggtt cctcaccttg atgatgcggg
     4681 tgacgaggag cgagatctcc tgcgggttgc acggcttgac gatgtactcg agggccccct
     4741 ccttcatcgc cgtgatcgcc gtgtcgaccg tcgcgtaggc cgtgatgatg acgatcgagg
     4801 cgtcgggacg gagcttcttc acctccatca tcgtctcgat gccgtcgatg ccgcccggca
     4861 tcttcaggtc gacgaagtag atggcgtagt cggcctcccg cgccttgtcg atcccctcgc
     4921 ggcccgaggc cgccgtgtcg acctcgtacc cgtcctcgcg gagccaggcc gcgagcgact
     4981 cgcgcatgac cgcctcgtcg tcgatgacga ggatccgcca cgtcgccttc atcccgtcct
     5041 cccttccggc ggccgcgccg ccggggtccg ttccttcacg agcgccgcgc acgaggcgca
     5101 gagcgattct cccttgcgat cgacctgaac gacgccggtc gagagcgaca tcgggcaggc
     5161 cgggtccgga cagtgcacga ggccgaacgt gtgcccgagc tcgtgcacgg cctccttgcg
     5221 ggcccgcgcg aggaggagcg gccggtcctc cgggagcccg gagaacgcct gcgagatccg
     5281 ggcgagcgag atgacggccg tcgggcctcc catctgcgcc tggccgaaga cgaagctgag
     5341 gaccgggacg aagaggtccc gcccggtcac gcccaggatc cggtcggcct ccggcggccg
     5401 ggccgaaagg agggccttca gcatctccgg cgcgctccac tggccccgcg cggcgtcgaa
     5461 ggccccggcc ggctccggga tcggcgcgag gcgcgcggtc tcccgcccga aggcggtcgc
     5521 gacgacgccc tcgacggcgg ccaggacctc gtcgtcggag gggccgatcg ggaccacggc
     5581 gaggacgctc acgccttcgg cgccgccgcc tctccgcgca ccgggagagt gacgcggaag
     5641 agggttccct ccccgggcgc gctctcgacc tcgatgtcgc cgtcgtgggc ctggacgatg
     5701 ccgtagacga cggcgaggcc gagcccgacc cccttcccct cgtccttcgt cgtgaagaag
     5761 ggctcgaaga tccgctcctt cagctcgggg ggcatcccct cgccgtcgtc gcgcacctcc
     5821 aggacgacct ccttcttcag cgcggtcgcc cgcgtggaga cgcggaggtg gcccgcgccc
     5881 ttcctccgcg tcgcctcgcc cgcgttcagg acgaggttca tgacgacctg ctggagctgc
     5941 gaggagtcgg cctcgacgcg cgggagcgcc gcatcgaggg cgcactcgac ctcgacgttc
     6001 atcagcttga gcttgtgcga gacgagcgag agggtccggc ccacgatcac gttcaggtcg
     6061 gtctcggagc ggacgggcgt cccccgccgg gagaaggcga ggaggtcgga gacgatacgg
     6121 ccggtccgcg ccgtctccgc gacgacctgc tcgaggtacc gccggaactc ggcctcccgc
     6181 cccttcggga cgccgtcgtc cttcaggatc cgctgcatca gcatcgagag gttcaggacg
     6241 ccggagagcg ggttgttgat ctcgtgggcg acgctggccg agagctgacc gagggaggcg
     6301 agacggtcgg cctgcatcag cttctgctgc gcgaccttca gctgctcggt cctctcgtcg
     6361 accttctcct cgagcccccg ggtgagctcg ttcagctcct ccatcgccag ggcgagccgg
     6421 cggcgcatct cgttgaagga gctcgcgagc gcctccagct cctcgctcga ggtgagcgcg
     6481 atcggacggt cgagcttcat ctcggcgacc tgccgggtcg cctccgagag ccgggtgagc
     6541 ggcgtcgtga cgaaccgccg cgtgaacgcc gccgcgcaga cgccgacgag gaggaccgcg
     6601 agagaggtga agagggcggt ccggatctcg atccggcgca cctgctggtc gacgagcttc
     6661 atgtcgaggc cgacgtcgag gacgccgagg acgttctggc tctccgggtg ggcgtggcag
     6721 gcggcgttgc tgcacgccgg ctcgttgtag atcggggtga ccatcgcgag cgtccggccg
     6781 ccgccgggac ggtggacgac gcgggcgcgc gtcggcgcgt cgaccctcac gagcggctgc
     6841 tcggaggcgt ggcagaggaa gcaggcctcc gccttcttgt cgacgacctt cacctcggag
     6901 cggggggtcg agaacatgac ccgtccctcc ttgttgaaga tccgcacgct ccggatcccc
     6961 tgcttggccg cgatcgtctg catcacctcg taggccgcgt cgcgctggtc ggcgagcatg
     7021 gcgtgccatg tggagctcgc gatcgccccc gagagctgct cgacgctctg gagcatcgtc
     7081 gcctgcagct gggtctcggc cgagcggacc gcaatgacgc cgttcgccac gacgacgagc
     7141 gagacgacga ccgtcagcga gaggatgagc ttctgggaga gccgcctcgg tccgcgcatc
     7201 gtccgtccac cccggccgtc acgcgccccg ggatcgggat cggacggggc cacgggtcat
     7261 cgtacaccgc ggggacgccg gaaccgctcc ggcgtccccg cgcgggcctc gcccggcccc
     7321 cgcgccgggt cagcggcccc ggccgatctc tccgagcgcc tcggccgcgg cctcgaggag
     7381 cgcctcggag cctaccggcg ctccgaccgc gcccttcatc cagaggtccg gcgagatccg
     7441 cccctgccgc gccatccgct ccacctcggg gccgacggcg ccggccttct ccacatggcg
     7501 ctcgatctgg aaagcgatca ggtgccccaa cgggtagtcg gggaggtaga ggaacgagtc
     7561 gatcatgtgg gagtagatcg cgaggatcgt ggagtccttc acgccgaaaa ccggggcgta
     7621 gaagcggttc cagacgttct tcgcgctcgc gaggacggcc tggtgcagct gggcgggcgt
     7681 cgcctcgggg ttcctgtaca tccagtgcca gacgttcatg tcgacgaggg cgacgccggc
     7741 gatctccgcc gtcccccagt atgtgttcag cgtggcgagc gcccggccct tcgcgtcgcc
     7801 cttcggaagc ccgaggagcg gcaggtcgcg ctcctggaac acgaaggcga tcgcttccgt
     7861 gaaggccgtg ttcgggactc cggcgaggag ggtgtggtcg atgtcgttca gcgagatcgt
     7921 ctgctcgacg ttgtggccga gctcgtggac ggcgatgttg tagcccttgt agtccatccc
     7981 ctccttgccg acgcgcgtcc gaaggtgcgc cttgtccccg cggcgagccg ctccccacgc
     8041 gtggcccgag ccgcgcgccg ggtcgacgac gacgttcgcc gcgatcgcgc gggcccgctc
     8101 ctccgggaag ccgaggtcgc gcaggatccg ggggatgtcc ttctcgaagg cctcgggcgt
     8161 ggggtacttc ctccgcgtga tctcgtcgag ctgcgcctcg gtgtacggcc cgcggtcgcg
     8221 gaagcccggg taccagatgt cgaacggctc gagcggccgg ccgagccgct ccttgacgag
     8281 cgctccgacc tgggcgaaga cgggagagaa gaggacggat tcgagcatcg cccgcacccg
     8341 ggcctctggg agctggcggt cttcctggaa gcgccgcgcg atgagggtcg gggcggacgg
     8401 ggagtacggg tcgacgagac gtgcggcccg gaatgtcgcg aggagcttcg cgtatcggcc
     8461 gacggactcg ggcgagacga agaccggcag gtccttcggg accggcggag cgcccggctc
     8521 gagcgaggtc gtcgccttca cctcgttcgt gaacgggttc cagtcgatcc agggcgagtc
     8581 gatcgcggac gtggggatcg tctggttgac gatccgctcc atcgccctcg cgatcgtccg
     8641 ctgcttcgcc aggccgtcct tctcgtcgcc atagcaggcc ttgatctcat cgcgcaggtt
     8701 ccagtgcgag aggaggcgtt tgccctgagg gaagaggcgg cggcccttct cgtcgaggag
     8761 gtgatggacc cggaggttgt actcggagat gtaccgcgag gcgtcggccg acgccgtcgc
     8821 gacggcctgg ttcacctcgg ccggaatgcg tttgccgaac ccctccgcga ggcgagtctc
     8881 ggcccactcc ctccggctcc acgacggccc cttctcgagc cgctccggaa gcgtcgtcaa
     8941 cgggaagttc aggaggaccg cgaacgcgag gcggttctcg aacaggtcgt cggcgacgtg
     9001 ggccgagggg tcccatcccg ccatcacgtc gtcgaacggg aggatctctc cccggtcgag
     9061 atcggagtgc tcgcggaggt cgcggacgat ctcgagcatg tgccctccga ccgactcgag
     9121 gacgcgttcc atccgggaga agagcgcgtc gagctgggcc ggatcggaga ggaagtgcgt
     9181 cttcgccaca tcggcgaagg cggcgtcgtt gccgtctgag acgcgccaga gggcggcggc
     9241 ctgggcgagc ccgcgcgtga tacggggccg cgcggcctcg ccgtgccgct cgacgagctc
     9301 cgcctcgagg gacttcagcg cgtctgggga gacggaaggg acggcggcgg cgctcacgga
     9361 cccgccgagc gagaacgagg ccgcgaggac ggccgcggcg aggacgatac gcatcgggag
     9421 acctccaacc gggagagtct agccttccgc gcgggacacg agccgcttcg gctcagcgtg
     9481 cgaggaggac gccgatcccg aggggcccgc cgaagctggg cggctccgcg acgccccagc
     9541 gcgcggcccg atccaggagc ttgcgcgcgg acgctccgtc gccgagcccg tccgggaacg
     9601 gaggccagag cgtcgcgagc gaggtccagg cgctcggggc gccgcgtccg accgccgggg
     9661 tcgcgaggag gagcgcgtct ccccgcgcga gcgtcaccgc gggcaccgcc tccgcgcggg
     9721 cgagcccgcc ggcgccgagc gtccccggcg cgaggaacgc tggctcgggc gaccgcgcga
     9781 acgggaccgg tgagggcgcc ccccctctgc gggcctggac cgtgcccggt ccgaggcgga
     9841 cgaccttcgc ctcgacgcgg ccgcccgaga cggtcgcgat ggcgccgacg aggccgcagg
     9901 cgagggcggg cgggacgcgt cggagcgccg gatcgggggc gcgcgtgtgg gcgaggaccc
     9961 gccgcgcacc gtcggacgcc atcgtgcctc gcacccgggt cagggcgtcg gaggagtcac
    10021 cggccgtcat cagggcggcg aggagcatct cgaggtgtcc ttgcgccgtg cgcaccgccg
    10081 cgagagggtc ggaggggccg gcggccgcgg cgcgacagaa ggtgcgggcc gcgacgaccg
    10141 ccgcaagccg gctcccgagc gacgactccg cgccggtcgc caccgcgagc gcgatcccgc
    10201 cggggaaggc gacggcgaac gacgcggcga gccccggccc ctcggcccgc ggacccgcag
    10261 ccgacgcgaa ccaggccgag gcgcccgcga cgtcgacccg ctcgcccgcc tcgtcgatcc
    10321 gcaggtcgta ggcggagagg tcgggccccg ggtcctcccg cggcgccgca ggcggggccg
    10381 gccggtgcgc cggaggcgcg gcctcggggg gccggccggc gggagcggca tcaggcgggg
    10441 ccggccgggc actccccttc gccgccgcgc gcggggcgtg ccgcagagag accaccgtcg
    10501 gcggcgccgc gcggcgcgcc tcctccgcgg cgagcttcgt cacccagtcg acgagccgga
    10561 gcagctcgac gacgcgcgcg tggaggccga acgcggcgaa cgaggacagc tcctggaggc
    10621 ttctctccgt caccgcgttc cgctcgtcct cgagctgtgc ggcgagccgc tgcggcagcg
    10681 tccgtcgaag gcccgggccg agcgcctcga acgcgacccc cggctcgggg tccgggaccg
    10741 gctcggcccg gagcgcgaag agcccctcga ggaggtgcct cgcgaagagg agccgctccc
    10801 gcagctcctc gatctcctcc gctccggcgt ggctccggcg cgagaggctg agcgccccga
    10861 cgcgctcgga gacctccccc agaatctcga ggagctccgg caggtcggcc gcgtcacgtc
    10921 tctcgtcgtc cctcacgtct cccccgtccg gatggtatgg ggagccggcg gccgagtcca
    10981 cgacgccggg ctatcctcgc ccgcgcgagg cgaccgcatg agccgaggcc cgtcccttcc
    11041 ggattcgctc gaccgctggg agcgcgcgcc ctccccggag cgcgccgcgg aactcctcga
    11101 ggccgctcgg gccgcgctct ccgaagggag tgcgcccccg gccctctggc accgctggct
    11161 cgacgcgact cggcggagcg agcacctcct cgccctcggc gaccgtgaga ctcgcgtcgt
    11221 ctgggccgag gtggcgatcg gggccgtcca cgcctccggc tacgacctcg cgcggctgct
    11281 cgcgcagcgc gtcgcggcgc acccggaacg ggctttcctc gtcgacggac cggcgccggg
    11341 agcgccgcgc tggagctacg cgcacgtggc gcgccgcgcc gacgcgatcg ccgccgccct
    11401 gctcgcgctg gaggccgagc cgcgcgtcgc gatcgtcgcc gagaacggcg tcgactcggc
    11461 gtgcctcgac ctctgctgcc tgctccacgg cctcctcgtc gcacccctct cgccgcacct
    11521 gatggccgga gagctggtcg ccgcgttcga cgcgctcggc gtgaacctcg cggtcgtcgc
    11581 ctccgaggag agcctccgga gggtccgcga ggcggcgctc cggaccgctc gcccgttccg
    11641 cgtcgtcctc ctcgacgagg cggccgagct cgcctccgag cgggacgtcg tcctcgggga
    11701 gacgctcgcg gcgcaggccg aggcctccgc cccggcgctc ctcgcgcgcg ccccgcggcg
    11761 cgcgctccac gagacgtgca ccgtcctctt cacctccggg tcgaccggcg gccagaaggg
    11821 ggtcgcgttc tcgatggcga acctcgtgac gaagcgcttc gcgcgggccg ccgcgctccc
    11881 cttcgtcggc gaggaggaga ccctcctctg cttcctcccg ctctaccaca cgttcgggcg
    11941 ctacctcgag ctgctcggga tgctcttctg gggcgggacc tacgtcttcg cggggaaccc
    12001 gtcgctcgac ggcctcgccg ccggcctccg cgccgtcgag ccgaccgggc tgatctcgat
    12061 cccgatccgc tgggcgcagc tggagtcccg cgcccgcgag gcggcgggcg aggcgccctc
    12121 ccccgaggcc tcggccgccg cgctccgctc cgtcgtcgga gggcgccttc gctggggcct
    12181 ctcggccgcc ggctacctgg ctccgcaggt cttccgcttc ttccaggcgc acggcgtcga
    12241 gctctgctcc gggttcggga tgaccgaggc gacgggcggg atcacgatga cgcctccggg
    12301 cgcgtacgag gaggggagcg tcggcctgcc gctccccggc gtccgcgtcc gcctcgccga
    12361 gcacgacgag ctccagatcg ccggcgccta cgtcgcccgc tacctcgacg agccggagac
    12421 ggcgccgggc gaggagcgat ggctcccgac gggagacgtc ttccgcgtcc gcccgagcgg
    12481 gcacctcgag atcgtcgacc gcgtcaagga catctacaag aactcccgcg ggcgaacggt
    12541 cgccccgcgg cgcgtggagt cgcgcttcga gggggtcccc ggcgtccgcc gcgtcttcct
    12601 cgcgggggac gggcgggacg acaacgtcct gctcgtcgtc cccgacgcgg aggaccctgt
    12661 cctcgcgggc gacccgcacg gggagacggc ccgcgattac ctgcggcgga tcgtcgccgc
    12721 cgcgaacgcc gacctcgcgc cgtacgagag ggtcgtcgac ttcgccgtcc tcccgcgcga
    12781 cttctcgctc gaggaggggg agctgacgcc gaagggctcg taccggagga aggcgatcga
    12841 gaaggcgttc gcgccggtga tcgaggggct ctaccggaac cgcgtcgtgc gtctcgccgg
    12901 cgacggcgtc gaggtccgcg ttccgcgctg gctcctccgc gacctcggcc ttctcgaggg
    12961 agacctcgcc gtcgaagggg ggcgactcgt cgacctccgg cacggccgct ccgccgccct
    13021 cgccgcccgc ggcgagggcg aggaggtccg ggtcgggagc ctcgcctacc gctgcgccgg
    13081 gcaggcggtc gacctcggcc tcctcgcccg ccagccgctc ctctggctcg ggaaccccga
    13141 gctggccgcc ctcctccccg tgaaggacgg ctgggacacg ccgctcaccg gcagctgccc
    13201 ggaggtcctc ctcccgtcgc gggaggaggt gcccgaaggg gccgacgccc cgctgccggc
    13261 cccgctcccc tcgccccagc tcacggcgct ccacaagctc tcggtcgcgc tcctcttcgg
    13321 cccgcccgag gggtccctcg cggcggtcgc acgcgccgag gcgctcctcc gccagggaga
    13381 ccgccggacg gccgagctcc tccgccgccg cctccaggcg ctcgcgcggc accccgacgt
    13441 ccgggtccgc tgcgaggcgt accgggtcct cctcctcgac gacccgctcg tcgaccccgg
    13501 cacgatcctc cccgcgtttc tccgctcggg cctcccgttc ctctcggagg agagcgtcgc
    13561 ggcgatcgcg cgggccgagc tcggcgagga gcggctcacc gcgctccggc ggcgcctcca
    13621 ctcctaccgc gaggcgatga cggggccgct gccggagacg acccgctcga cgctgggcga
    13681 cgtcctcgag ctcctgggcg acttcgcgcg gcatcacccg gaggaccacg cggccgtccg
    13741 cgccgagctc gcctcctggg cgatccagga acgcgacccc gagctcgcgc gccgggccga
    13801 cgcgctcttc gacgcgctcg gcgtctggca cgaggagacg gccgccgccg cggcgctcct
    13861 cccgcccgtc tccgagggac ggaagaagct ctccttccgc gacggcatgc cggaggagga
    13921 ggtccgcacg ctggaggggc tcctcctcgg gacctcgttc ctccgtgagt cgatcgagct
    13981 cgccttcgag accgacccgc ccgagctcgc cgacatcccc gacgagggga tctgggtgac
    14041 gccggtccag gcgctccagc gccaccgcct ctatcggctc tcggtgagga ccgcctccgg
    14101 gaatcaccac gacctcctcc tgacggtccg gggagacctc gactcggccg cggtccgcgc
    14161 gacgacgctc cggatgatcg cgctcgccgg ccacccgtgg ggcccgccgg tcgtgccgcg
    14221 cgtcggctcc tgccgccccg acctcggcgc cctctcgctc gcctgggtcg acgagctcac
    14281 gctcgccgag cgcctccgcg acgccgccgt gccggggcgc gcccgtcccg gcgaagggca
    14341 ctccgtgaag agcctcgtcg tccgcgcgct cgcggcgttc ttcacggcct gggaccgcag
    14401 cggccggctc ctcgtccccg gcctcgtcgc cccctcgaac gtcgccgtcc cggcggagga
    14461 ctaccgcgac ggggcgcgcc tcctctcgat ctccggcgcg cgggcctaca cggggcctct
    14521 cgacctcgtc gcgccgatgc tccgcaacgc gttccgggag ccggccgccc tctcgccgcg
    14581 cctcgccgcg gtcctctcgc ccgcctggat cgtcgacgcc gtcttcgagg cgctcggaga
    14641 ggcgggagcc cggacgttcc tcaccgagct gagggaggcg gccggcgccc cgtcctccga
    14701 ggcgtgggag cggctccgga ccgcggtcga cgccgcgctc gcggcccccc ccggagaccg
    14761 gacgccgctc gccctcgaga ccgccgtcgc ccgctacgag gcgtgggagg gcgagcgccc
    14821 cggcgtctcg ctcgccgagc gggagcggac cgccgagtcg ctcctccggc tctatcgcgt
    14881 cgacgcgctc ggcgaggagg cgcgctaccg actcttccgc gcgacggttt tcgcccgcag
    14941 cgcgccgccg gtcctccggg cgttcgacgc gctcctcgcc gcccttcgcg cgcgccccgg
    15001 ctcgcgcccg acgcatctcg tcgagctgca gggcctccac gcgacgctct cgacggacgc
    15061 cgagaggcac gcgttcacgc ggctcgtctt ccccgccgcg cgctccaccc gccccgtgga
    15121 ggtgagcgtc gtcggagacg ccgtcaagca cgtcgtcgtc gcgacggagc tcgtcgaccg
    15181 gatggacggc gcgtggctcg tccgcgagcc ggtcgacgcg gccgaggtgg gccggctcta
    15241 ccgcctcttc gtgagggccg gcctcccccg cgcgttctct ccggaccgcc gctacctggt
    15301 cgtcctcgac gcgtcggagc ggatcgtcgg cggcgtctcg tggtcgcagc tctcccccga
    15361 cgcggcgcag cttgacggcc tcgtcgtcgc gccgagcgtc cgcggccggg gcctctcgag
    15421 cgcgcttctc gacgaccttt gcgcgcgcct cgcctcgctc ggggtccgct ccctcctcgc
    15481 ccacttcgcg ttccggaacc tcccgcttcc cggcttccgg ctggaccgcc gtcacggcgg
    15541 cctcgtccgc gacctttcgg ccggcctcga ggtggaggcg gggctggcgt agagtcccgc
    15601 ccgtgaagca gctgcaggag cggctggagc tccggacgcc ggggcgcggc ctccacgagg
    15661 tgaccggggc ggtcgccgcc gtcgtcgccc gctcgggcgt cgcgaccggc ctctgcgtcg
    15721 tcttctgccc gcacacgtcg gcgagcctcc tcctgcagga gaacgccgac ccgacggcac
    15781 gcgccgacct cctcacgtgg ctcgcccgcc tcgccccaga cggcgacccg cgctacgcgc
    15841 acgacgcgga aggggacgac gacatggccg ctcacctccg gagcgccgtg acccgctcga
    15901 gcgagacgat ccccgtctcg ggcggacgcc tcgccctcgg gacatggcag ggtctcttcc
    15961 tcgccgagca ccggacgacg ccgcaccgcc ggtcgctcgt cgtccacgtc ttcggagagt
    16021 gacgtgaggg gcgcccgttc ggcacgcgcg ctccccgccg cgctcgcagc gctcgcgttc
    16081 ctcgctctcc cggccgctga cgcttcctcc gcctcgctcg cgccagtttc cggttcgtcc
    16141 tcgctcgctc tcgcccccgg cttcgtcatc gcggcctcgt accgctcccc cgcggacggc
    16201 gccgagcgcg gctatgcgat ccgcgttccg cggggctggg acgggacgaa cctcctcccc
    16261 gcgatcctct tcctgcacgg gaggggtggg accgtccggc agttccagcg gactccctac
    16321 ttcgacgccg ccgacgcccg cggggtcgtc ctcgtcttct gggaggggcg gatcgcgctg
    16381 ggcggggccg aggtcccgtc gacgcactac gtcgacggcg cggacggcgt ccccgacgag
    16441 accgacgtcc tcgcctgcct cgacgacgtc ctctctcgcg cgccggtcga cgccgagcgc
    16501 gtcacgctcg ccggcttctc gcagggcggg cgcggcgcgc tcgggatcgg cctcctcaat
    16561 ccctcccgtt tcgcggccgt cgtcgacgcc gccggcccga ccgacgcgtt ccagggccag
    16621 ctctggtcgc cggccttccc cgactacctc gccgccgcag gcggtccgcc gtcggcggga
    16681 ggcgaggtcc tcgcgcgctg gcacgagctc tcgccgcgct tcctcctccc gaacgcccgg
    16741 aacctcttcg tcgcggtcct ccacggccag gtcgacgacg tggtgcccga ctccgcggcg
    16801 tacttccctt accgcaactc ccaccacgtc accgtcacgc ccggcttctc cgacgcccgc
    16861 ggccggacgc cgacgctcgc cgagctccac gccgaggacg agggcggcta cgccttctcg
    16921 accgacttcc ccgacgacgt cgggcacgac caggtccggc tcctcaccgc gtccgcgctc
    16981 ttcgaggccg cgctcgggaa ggtgcgcgcc tcacggcccg agcgcgtcgt cggcgtctcg
    17041 ttcggcgcgc gggagcggac gttccactgg gcgcgcctcg ggaggaggat tccgccggac
    17101 ggcacccgcc tcctcgtcga cgcgcgctcc gacctcccgg agaatcgcgt cgacctccgc
    17161 gccgaggggc cggcccgcgt ccgcctcgac cttccggccg ccggcctcga cgccgcgcgc
    17221 gccctgtctg tccgcgtcga cgccgtcggc gcgatcgacc tcgcgctcga cggaccgttc
    17281 cctcccgccg tcgcggcgac gctcgacggc cggccggtcc cggtggaacg gacggcggag
    17341 ggagtcgccc tgcccggcct cgcgcccgcc gcccccggcg cccttctcgt cgtcgcgccc
    17401 gctccggccg ggccggtcgc ggagggggac cggctcgtcc cggcgctcgt ccggacggag
    17461 ggcgccaacg gcgcccgcta cgacaccgtc gtcacgctgg cgaacctctc ggacgggccc
    17521 ctgtcgctcg aggcgctcct cctggacgga gcgtcggctc ccgtccctct cctccttccg
    17581 gccgggacga gccgtgccgt gcccgccggg acgctcggcg ccgcgtcggc cgcgttcgcc
    17641 gctccgcttc ggctgcgcgt cctctccggc gagggcgacg cgctcgcggc ctccgcccgc
    17701 gtcttcagcg cgtcggacga cggcacgtac ggcctctcct tcccggtgct tacggccgga
    17761 gagagcgtcc tttcggccgg cgagaccgcg ctcctcttcg gcccgcgcga cccggcatcc
    17821 gagcggctga acgtctcgct cttcgccccg ttcgaggcgt cgacggccga ggtcgccgtc
    17881 ttcgacgggc ggggcgtcgc cgtccggacc cggcccgtcg cgctggccgc tctcgcgcgc
    17941 gtgcagctcg acgacctcct ccgcggcgag gcgcccggcg cccacgtgag ggtctcggtc
    18001 ttctcggggc gcctccaggc ctacggcacc gccgtctcga acggcccgac gaacgacccg
    18061 tggcgcgtcc ccgcgcttcc gctcgccgcc gcggcgaaag cctggaccgt ccccgccgtc
    18121 gcgtcggccg aaggacggaa cggcgcgtac ttccggagcg acctcttcgt cttcgcgccc
    18181 gagggagcgt ccctcgacgc gacgctcctc ccacgcgacg gcagcccgca cgagaaggcc
    18241 ggcctcctcg tgccgggcgg cgcgccgcac gtcgtcgcgg acctcctcgc ctcgctcttc
    18301 ccgtcgaagg cccccggcgc cggggcgctc ctcctctcct cgacgtcccc gttcctgccc
    18361 ctcgccgtca cccggagcga gccgccgacc ggcccctcct cgcaggacct cccctgcgtc
    18421 ccggagggcg gcgaggtggt ccccggccgt cccgtcgctt tcgccggcgt cgacgagtcg
    18481 acctcggccc gctcgaacct cgtcctcgtc gcgcccgcca ccgccgcgcg cgtgaggctt
    18541 cgcctcctcg ctccctcggg ccctctcggc gagcacgtcg tcgatctccc cgcgggccgc
    18601 gtcgtccagc tcgactccgt cgcctcgcgg ttcaccgacg atccggtcga gggagggacg
    18661 cttctggtct cctgcgagag cggcgccgtc gtcgcgtcgg tcgcccggat cgacgcggcg
    18721 acgaacgacc cggccggcct cgcgccgctg ccggtcgtcg tccgctgaag ctcaggctct
    18781 ctcgagcttc gccttctccg tcgcctccag caggaacccg gcccggagga ggtagtacca
    18841 ggcgtggagg agcgcgacgg ccaccccggc tcgaccgtcg aggaacccgc gcttgaggac
    18901 gtacgaccgg aggaactcga gcgggggccg gaggacgagg aggaggacgc tcccgcggcc
    18961 gccgcgctcg aagcgctcga gggccgagag gcgcgcgtag aggacgttct tccggaccgc
    19021 gtcggcgagg tcgcggtacg ggtgatggag gaggggccgg gaaaggtcgc ggacctctcc
    19081 gtcgacctcg atccactcgt ggacgtgccc gcccgcggcc cgcaagcccc gggaacgccg
    19141 gccgagacgc agcttccggt cgggccacca gactccgtgc cggatcgggc gggcgccgag
    19201 gtgcgagagg cgcggcatcc ggaaggcgtc cgcacctttc gggtcggcga cggcctcgcg
    19261 gatctccgcg gcgagctccg ccgtcacggc ctcgtccgcg tccaggacga gcacccagtc
    19321 gttcgccgcc aggccgatgg ctcggttccg cgtggcgacg tagccctccc acgggaccgt
    19381 ctcgacgcgc gcgccggcgg cgcgggccag ctcctgcgtc ccgtcggtcg agccggtgtc
    19441 ggcgacgacc acctcgtcgg cccaggagac ggaggcgagg gagggggcga gccggtcggc
    19501 ctcgtccttc acgatgaaga cgacggagac ggcggcgcgc atccctcgat tatcctcgac
    19561 ggcgtgaggc tcctcctccg cgcgacgaac tgggtcggcg acgtcgtcat ctctcttccg
    19621 gctctccggg ccctgcgcgc gcaccaccgc ggcgaccaca tcgccgtcct cgcccggccg
    19681 tgggtggccg agctctaccg actcctgccg gaggtggacg agctcctcgt cgaggatccg
    19741 aagggacggc acgccggcag cgagggacgg cgcctcctct ccgacgagct ccgggagaag
    19801 cggttcgacc gggcgatcct cctgccgcgg agcttcacga cggcctggac cgtctaccgg
    19861 gccggcattc ccgagcgctg gggttaccgc ggcgagctcc gctcgccgct cctcacccac
    19921 cccgttccgt tctctccgcg cgccggggag cacgagttgc atcggcacct ccgcctcgtg
    19981 gaggcaacgg gcgtgccggg cccggcgatc cccgacgcga cgctcgccgt ccccgactcc
    20041 gtccggcggg gcgcccgcgc gaagctcgcg gaggcgggcc tcgacggcgg cccgttcgcc
    20101 gcggcccacg tcgcctcctt cgcccatgag gcgaagcgct ggcccgagga gcggttcgcc
    20161 gcactcttcg cgcacctcca cgaagagcgc ggcctgccgg tcgtcctcct cggaagcgcc
    20221 gcggaggcgg cgatgaacgc ccgcgtcgcg gccctggccg ccccggcgca cgtcgtcgac
    20281 ctcgcgggga ggacgtccct cgccgacgtc ctcggcctcg tcgccgaggc ggagctcttc
    20341 gtcgggaacg actcgggcct cgcgcacctt tcgaacgcgg ccgggacgcc gacgaccgtc
    20401 gccttcggcc cgaccgaccc cgacgccacc cgcccctggg acggcccgcg ccccgacggg
    20461 aagcccgtgt cgcttcggat cgcgcgcaac cgcgtcccct gcgccccctg ccgattcaag
    20521 cgctgccccc tcgatcacgc ctgcatgacc ggcctcgacc tggggctcga ccaggggtca
    20581 ggtcttgttt tgtgactttc gcgaaaccgc gaaagtcgca aaacaagacc tgaccccctc
    20641 catcaggcgt cgagcggtgc gcagaggagg tggaggacgg cgagcgtgat ctcctggacg
    20701 tgggccgtct cggccgacgg cacgacgagg ccgtggtccc acttcgctgc ctcggcggcg
    20761 ccgcgcgtcc cggtgacgag gatcgtcact atgccgcgcg cgcgggcggc ctcgagcgcc
    20821 ttcacgacgc tcgggctcgt gccggaggtg gaaagggcga gggcgacgtc tccggggcgg
    20881 ccgagcgcct cgacctgccg cgcgaagacg tagtcgtagg agtagtcgtt cgcgcaggcg
    20941 gtcaggatcg cgccgtcgca gacgagcgcg accgcgggca ggcccggccg gtcccccttg
    21001 tagcggacga cgagctcggc ggcgaaatgc tgcgcctgcg tggcgctccc gccgttgccg
    21061 aaggcgaggg tcttgttccc cgcccggacc gccgccgcga ccgcggccgc cgccgcttcg
    21121 atcgccggct tcatcgcccc gcgcgcggcg agggccgtct cggcgagggc cgtaaggtgc
    21181 gcgtcgatca cggcgcctcc ttcattttcg acaccttcgt gaccatggag gtcgtcgagc
    21241 gtcccggcac gagcgggacg agcgcgacct cgccgccgta ggaacggacg aacggcgcgc
    21301 cgacgaccga ctcctcggtg tagtcgcccc ctttcacgag gacctgggga cggatctcgt
    21361 ggatgagcgc ctcgggcgtg tcctcctcga agaggacgac gaagtcgacc gcgtcgagcc
    21421 cgccgaggac ctgcgcccgg tccgtctcgg agaggatcgg ccgctcgggt cccttcagcc
    21481 tcttcacgga cgcgtcggag ttgaggccga cgacgaggac gtcgccgagc cgcttcgcgg
    21541 cgccgaggag cgtgacgtgc cccgggtgaa ggaggtcgaa gcagccgttc gtgaagacga
    21601 cgcgccggtg cgcgcgccgg agcatctgcg cgacggccgc gacgtggccg cgatcgagga
    21661 gcttctcggc cggggaggcg gtgaggccgt cgaggaggtc ggcccagcgg acggacgcgg
    21721 tgccggcccg cccgacgacg acgcccgccg ctcggttcgc gacctgggcc gcgtcgccga
    21781 gcgggaggcc cgctccgagc gcgatgccaa gcgtggcgag gacggtgtcg cccgcgcccg
    21841 tcacgtcgaa gacctcgcgg gcgcgcgcgg cgatcctctg cggcgcggcc cctttcggca
    21901 ggaggagcat cccctcctcg gagagcttga cgaggatggc gtcgaggccg aggtcgtcgc
    21961 gcagccgcgt cgcgaggcgc gcgacggcgg cgtcgtcgcg cgcgctctcg cccgtcaccg
    22021 tctcgagctc ctttcggttc ggcgtgacgc acgtggcgcc gcggtaccgg ccgtagtccc
    22081 gccccttcgg gtcgacgagg gccgggatgc cccggcgccg cgcctcggcg agaagggtct
    22141 ccatgaggcg cggagtgagc gtccccttcg cgtagtcgga gaggaggagg acgtcggccg
    22201 actcgagcgc ggcgagggcc gcgccgatca gctcctcctc ggccccctcc gagaggacgg
    22261 cggcgagctc ctggtcgacg cggaggagct ggcgcccgtc ggcgaccatt cgccgcttga
    22321 ccggggtcgt ccggagcggg tcgacgacga cgcgcccctc gccaccgggg agcgagccca
    22381 ggtgtcgggc gatccaggcc ccctcctcct cgggtccgac ggccccgagc gcgaccgtcc
    22441 gggcgccgag ggccgcgagg ttctcgacga cgttccccgc ccccccggcc cgccggcaca
    22501 tcgacgagag agcgaggagc ggcaccggcg cctccggcgc gacccgcgaa acgtcgccca
    22561 gccacgtctc gtcgagcatc aggtcgccca cgacgaccgc cttcacgctc ggcgccgcgg
    22621 cggccagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga gcatcaggtc
    22741 gcccacgacg accgccttca cgctcggcgc cgcggcggcc agggcagcca gctcgcgcgg
    22801 cgtcacgacc tcttccctcg cgcggaagaa gtccgggggg accccattcg atgggcttcc
    22861 ccgaggcata gcgtcgaagg ggtcgtgcct gcgtcctcca gccccaggac gagcgcctgg
    22921 gcgaggagcg gcaggatcag ctcgtggtgg cccgtgatcg cgtacgacgc tccgcccgag
    22981 aggacggggc gtttcaggac gttctcgtgc gggcggtagt ggcggttcat gtcgaggtcg
    23041 acggtcgtga agttcctcac gtcgtgcccg aggttgcggg ccatcgagag ggccttcagg
    23101 aagacttccg gcatgatgac ggccgacccg gcgttgatcc agaccccgcc gtcgccgagc
    23161 ccggcgatca cgcgccggat cgtcgtgaag tcgcggatcg acatctcgcc gacgagcgcg
    23221 ccgtccatcc ccggatgctg gacgagggtg tcggtcccga gagcgacgtg gacggtgagc
    23281 gggacgtccg ccttcgccgc cgccgcgaag agggagaggt ggcggaacgg gaggcgggcc
    23341 cggaggatcc cctcgccgag ggcgcggccg tagccgacgc cgttcttccg ccccgcgcgg
    23401 agggcgcgaa agaccccgac gcccgtctcg cggctcatcc cgaactcgcc cgtctcgagg
    23461 accggcccga cgtcctccga ggtcttcccg tggaaggcga tctcgaagtc gtgaatgccg
    23521 ctcgcgccgt tcgtcgcgag cgccgtgacg aagccgcgct cgacgaggtc gaccaggatc
    23581 gggccgaggc ccgtcttcag gacgtgggcg ccgaaggcga cgacgacggg cttccccttg
    23641 cgccgcgccg cgtggatcgc ggcgacgacc ttccggagcg actcggcggc gaggattcgc
    23701 ggcaggccgt tccagaagtc ggcgaaggcg ccgctcgtcg agggcttcgc cgcgaagtcg
    23761 tcgaccttca ccttgaacga tcgcgtcgcg atcggccagg tcgtcaccgt ctcgcgcggg
    23821 cgtcggcgtt tcgtcgtcgt catccctgct tcccttccgc cgccgcgcgg agcctgcgga
    23881 ggccgtcggc gagccccccg gcgaggatca cccgcccgct ccccttctcg cgcacgcggg
    23941 cccggtgctc ccggccgtgg cccgagagga ggtggaccgc gatcgcgccg agcgcgagcc
    24001 ccgcgccgac gtcgtcccac ttgtcgccga cgacggccga ccgctccgcc gcgagcccgt
    24061 gccggcggag cgccctccgg tggaggagcg tgcccggctt ccggcagcgg caggcgacgg
    24121 cgtactcctt cacccgcccc tcggggtggt gcgggcagac gagccagccg gcgatcggca
    24181 caccgcgcgc ggcgagccgc cgctccacct cggcgttcac ggcgttcacc tcgtcccagc
    24241 gggtcagccc ccgcgcgacg cccgactggt tcgtcacgac gaagaggcgg gtcccctccg
    24301 cggcgaaccg ggcgaggagc gcagcggcgc ccggcgcgag ccgcacgccc ttcgggtcgg
    24361 cgaggaagcc ccgctcggcg atgagcgtcc cgtcgcggtc gaggaagagg gcgtcgagga
    24421 ggggccggcg cctcacttcg cgccgacggc ccttcggatc gcctcggtga aggaggcttc
    24481 cgtctgaagg cccgagagac gggccgcgat cttcccgtcg cggccgtaga tcagcgtgta
    24541 cggcagcgag ccgtcccacg tccggtcgac ggtgtcgatg aagtcctgcg ggtcgcgcga
    24601 cttcgcgagc cagcagacga acgggacctt ctgctccttc aggaacttcg ggacctgcgt
    24661 cgggcccgtc ttctgcgagt cgagcgagac gagggtcacc gcgaggtcgc gcgaggggaa
    24721 cttcctcgcg gcggcgacga ggtcgggcat ctcctcccgg cacggctcgc accaggtggc
    24781 ccagaagttg acgacgagga ccctcccctt cttgccggcg acgacgcgcg cggcgtagtc
    24841 gcgcgggagg aggtgcttga ccggctcggc cgacgcggag accgcgccga ccaggagggc
    24901 cgccgccagg acggcggcgg ccgcgctcct cgggttcgcg gagacgttcg gtccggccac
    24961 tctcagacgc gcttgatcgt gcacccgaag gccttcgtct cggccgccgg gacgggcttg
    25021 ccggccagga tcgcctcgag ggcgttgatc aggtcgggcg acttcacgtt cttcgggtcg
    25081 tcgtgggtct cgtcgatccg gccccggtag aggagcgtcc ccttcggatc gaggacgtag
    25141 atctcgggag tcttctgcgc cccgtagaga tcggccaccc tgttcccctc gtccttcaga
    25201 acggggaatg cgaagccgtg cttcttcgcg tcgtcggcga cctcggccgc gggctccgtc
    25261 ttgttcgagt tgatcgtcac gaaggtgatc cccttcgcgg cgtactcctt ccccatcgcc
    25321 gcgacgcgct ccttgtagcc cttcgcgtac gggcacttcg tcgcgtcgaa gatgacgacg
    25381 acggccttgc tcttgccgag caggtccttt agggcgaccg cctccccgcc ggcggcgggc
    25441 gggagggtga agtcgggggc gacggcgccg atcttcggcg cggcgggcgg ctcggcgggg
    25501 gcggtgcacg cgagggcgag cgtcgtcccg aggagggcga gggcgaggcg gggcgtcttc
    25561 attccgtgac ctccatcatc ttcccgaagg cctcctcgga gaccctcgtg agcgtggaaa
    25621 cgggaacgtc gaggacgaca ttcccgctca cggccacccg gaggctcccg cgggagaccc
    25681 ggccgaggaa cgccgtcggg acgccgtgcc ggcgggccgc ctcgaggacc gcccgctcgt
    25741 ggccgggcgg gaaggagacg acgacccgcg gggtcgactg ggagaagagg tagatcgccg
    25801 ggaggagcgc cgtctcgacg ttcaggtcgg cgccgaggcc gttcgggatc gccgactcgg
    25861 cgagcgcgac ggcgagccct ccctccgaca cgtcgtgcgc ggaggagagg agcccgtccg
    25921 ccgcgagggc gacgaggagg tcgaccatcg acctcaccga gaggaggtcg aggagcgggc
    25981 aggggccctc gtcgcgcccg aggacggtcg cgaggtactc gctgcggccg agctcgtcgc
    26041 gcgtctcgcc gaggagcgcg acgacgtcgt gctcggcgcg gaaggcgtgc ggcaccgccc
    26101 gggcgacgtc gtcgagaagg cccaccatcc cgacggtcgg cgtcggcagg atcgactccc
    26161 cgtccgtctc gttgtagagg gagacgttcc ccgagacgac gggcgtctcg agcgcgcggc
    26221 aggcgagcga gagccccttc accgccgcgg cgagctggcc catgatctca ggccgctcgg
    26281 ggttgccgaa gttgaggcag tcggtgatcg cgagcgggcg cgcgcccgtg caggcgacgt
    26341 tcagcgccgc ctcggccacc gcgtgggcgg ccccgcgcat cggatcggcg aagcagtagc
    26401 gcgggttcac gtcggccgtc agcgcgagcc ccttcttcgt ccccttcacg cggaggacgg
    26461 cggcgtcgcc cggtgggccg acgaccgtgt tcgtcctcac gctccggtcg tactgccgcc
    26521 agatccaggt cttgctgcag aggttcggcg aggcgaggag ggcgaggagg gcggccgtct
    26581 cgtcgggggc gccggagagg tcgatcgccc cttcgagcgg cgggagcggc tcggcgagcg
    26641 tccacgggcg ccggtagacc ggagcgtcgt cgacgagcgg cgcgacgggg agatcggccg
    26701 cgacctcgcc gtgccatcga agcaccatcc tcggctcggc cgtcacctcg ccgatctcgg
    26761 cgacgtcgag gccccacttc tcgaagacct cgatcacctc gcgctcgcgc cccttcttcg
    26821 tgacgaggag catccgctcc tgcgactcgg agagcatcag ctcgtagggg gtcatccccg
    26881 actcgcgcat cggcacccgg tcgaggtcga ggcgcatccc gacgtcgccg cgggcgcaca
    26941 tctcgaacga ggacgaggtg agccccgccg ctcccatgtc ctggatcgcg acgacggcgt
    27001 ccccttcgag gacctcgagg accgcctcga ggaggagctt ctcggtgaac gggtcgccga
    27061 cctggacggt cgggcggcgc tgggacttct cgtcgtcgaa aacgtcggag gccatcgacg
    27121 cgccgtggat gccgtcgcgc cccgtcttcg agccggcgta gaggaccggg ttcccgacgc
    27181 ccgaggcctt cgccttgaag atcctctcgt ggcggacgag gccgaccgcc atcacgttga
    27241 cgaggatgtt cccgtcgtac gacgggtgga agaacgtcga cccgccgacg gtcgggatgc
    27301 cgatgcagtt gccgtagccg ccgacgccgc ggacgacccc gtcgatcagg ccgtgcatcc
    27361 ggggagccga agggtcgccg aagaagagcg ggtcgaggac ggcgatcggc cgcgccccca
    27421 tcgtgaagac gtcgcggagg atcccgccga cgcccgtcgc cgcgccctgg tacggctcga
    27481 tgaaggacgg gtggttgtgg gactccatct tgaagacgac cgcgagccct tcgccgatgt
    27541 cgacggcgcc ggcgttctcg cccggcccct ggacgaccct cggccccttc gtcgggaggt
    27601 cgcggaggaa gaccttcgac gacttgtagg agcagtgctc gctccagagc gccgagaaga
    27661 tcccgagctc gacgaggttc ggctcgcgtc cgagggcggc caggatcctc tcgtactcgt
    27721 cggaggtcag cttgtgctcc gcgaggagcg cgggggtgat cgtcggggcg gaagctccca
    27781 tggcgccgga gtttacccgc gcgcctcgtc gcctccggga gggtaacctc cgcgggatgt
    27841 tctcgaggag gacccccgct cccggcgccg agaaccggct gacgcaggcc ctcgcggcgc
    27901 acccgcgccc cgtcctcgac ctgaccgcca cgaaccccac ggcggccggc ctccctctcg
    27961 acgccgggcc cgcgctcgcc ctcctcgccg acccgcgcgg cgcgcgctac gaccccgacc
    28021 cgcgcggcct cctctcggcg cgcgaggccg tcgccggcca ctacgcgggt cgcggcgtcg
    28081 tggccgaccc cgcgcggctc ctcctgacgg cctcctcgag cgaggcctac ggctggctct
    28141 tcaagctcct cgcggagccc ggcgacgcgg tcctcgtccc ggcgccgagc tacccgctgc
    28201 tggacgcgct ggcggcgctc gaaggggtcg agctcgtccg ataccgcctt ccggccgagg
    28261 accgctggac ggtccacgcg agcctcgtcg aggaggcggc ggaccgggcg gaggccgccg
    28321 ggcgccgggt cgccgccgcc gtcctcgtca acccgaacaa cccgacgggc acgtcgatcg
    28381 ccgcggagga gctcgacacg ctctccgccc tcgcggcccg ccgcggcttc gccctcgtct
    28441 ccgacgaggt cttcctcgac taccgcttcg aggaccggcc gggcgacgtc cgcgtcgccg
    28501 ccgccgagcc gggcgaggcg ctcgtcttct ccctcggcgg gctctccaag tcggccgcgc
    28561 tcccgcagct gaagctcggc tgggtcctcg cgaacggccc ggccgggccg ctcgccgagg
    28621 cgctcgagag gctggcctgg atcgccgact cctacctttc cgtcgggacg ccggtgcagc
    28681 tcgccctgcc gggactcctc tcgttcgggg aaggcgccgc ccgcgtgctt cgcgcccgcc
    28741 tcctcggaaa ccacgccacc ctcgccggcg ctctggcgcc gctcgccggg gtcaccgtct
    28801 tccccctcgc cgcaggctgg tccggggtcg tccggctccc ggccgtggag cgggaggagg
    28861 agctcgtcct ccggctcctc ggcgaggagg acctcgtcgt ccaccccggg tacttcttcg
    28921 atttcccgaa cgaggcgtac ctcgtcgtct ccctcctccc cggagaggag accttccgcg
    28981 agggggccgc gcggctggcc cgcgcgatcg accgcccggc ctgacgggcc gacctcagcg
    29041 cggcttcttc ttcgccggcg gccgcttccc cttcggcgcg ggagccgcct tcgccggagc
    29101 cttcgggagg acgagcagct cgagggtcgt ccgccggacc tcccagtccg ccttcttcca
    29161 gatctcgcca cgcgtccggt cggccttctc cgtccggcgc accgcggccg ccgacaggag
    29221 gagccgtccc tcgcgctcga gcgggatcga ggcggtgccg tgcgcgtccg tctcggcctc
    29281 gagcgccgcg ttcgcccccg gggcgaacgc gcggacgagg gcgccggcga gaggccggcc
    29341 atcgaggagg agcgtcgccg ccaggacgtc tcccgcgcgc agagtgagcg gcgagccgcc
    29401 ggccacgagc tcgagcggga ggcccagcgc ctcgtcgcgc cccgccgccg ggtccgccgt
    29461 ccgcttcgcg cgcggcggcg cgacgacccc ggcgaaggcc cgggcgctct cgatcgtcac
    29521 gacccgcgcc ggcttctttg tctccttctt ccgggcgcgg agcgccagcg cctcgcgcgc
    29581 tcccgcctcg cggaggaacg tctcgaacgc cgccgcctcg accgtccgcc cctccgggag
    29641 gaggaggagg ccgagcgtgg cgaggccctg gccgagccac gtcgcctcga gacgcgtgag
    29701 ggggccgtcg acccgcgcgc cggtgagcgg catcggacgc ccctctccga ggcgcgcgaa
    29761 ggagcgctcg atccgctcgg gggggaccgc gctccccggc tcggggtagc tctctcccgt
    29821 cgtcacctcg acgaaggagg tgtctcccga gacggccgtc gggaagggcg ccggtcgggc
    29881 cggcggggcg ggacgtgcgg ccctcccgcc ccggcgggcg gcgggagccg ccttcacgac
    29941 gctctcgggc aggaccgggt ggagccacgt ctcggccgtc gtcgcccctg ccgcgccggt
    30001 cgcgctcgtc gcgccgagcg ccaggatgac gagggcgatc acacgcccgg gattcccgat
    30061 cgtcgcctcc cgcgtcggct ctctccgcat ctcccctccc atccgaaccg cggattctag
    30121 ggccgcccgc ctcgacccga gggctcgaag gcacccgcct agaatcgaac cgtggcccgc
    30181 ggcgagaaga agagtcggtc ccgcgcccgg cgcgtcctcc tcggcgtcgt cacgctgctc
    30241 gtcgtcctcg ccgccgcgac gatcgccctc ctgaaggcgc cgccagtcgg gcggcggatc
    30301 gtcgacgcgg ccctgcgcag cgccggagcc gcgtcgggcc tcgcgatctc ggcgcgggag
    30361 cgctcgctcg acgcgctcgg tgggaggctt cgcctcggcg gcctcgtcgt cggcctgccg
    30421 ggagagccgc ccttcctctc cgtcgacgcg gccgaggccg agctcctcgt cggcgaggcg
    30481 atccaggggc ggattcgcct ccgcttcgtg actctcgagg gggtccgcct cgacttcggc
    30541 gccccctggc cggagtcgga ggggcccccg gcgaccaacc ttccgttcct ctccgcggcc
    30601 cagatcgagc ggttccggat cgaaggcgcg acggtccttt ccggaccgct gcccgaggcg
    30661 ctccgcgcct tcgccctctc ggcgtccgcg tcggacgttc ggctcgacgg cgacctgcgc
    30721 aacgggacgc tccgcctgcg cggagtgacc ccccggatcg tcgtcgaaag acccggcggg
    30781 ctgcgcctcg aagcgaaggg ggacgtcgcc ctgacggcct cggccgacgg agagctcgtc
    30841 ctcgaggcgc tccacgtcga tggagaaggc ctcgctctct ccgcgttcgg aggtggagga
    30901 ctctcgcccg aggcgtcgct cggcctccgc gcggaggcga ccctcgaccc ggcccgcctc
    30961 gcgccggaga tgggggcgac gggctcgctc cggatctccg cgaagatgga aggacgccgc
    31021 gcggcaccga cggtcgaggc gacgctcgag gggcgcgacc tcgcgacgga ggagctcgcg
    31081 atcgccctcg ccacggcgcg cgtccgcttc gcggacgaga cgctcctcct cgagtcggcg
    31141 cgggccgacc ttcgcgacgg cggccgggtc gagggcgaag ggcgatacct cttcgcgacg
    31201 ggcgacggga gctgggacct cagggcgacc ggtctccccg acacgctcct cgcccgatac
    31261 gccgacccgg cgacgcgcga gcgcctcgga atcgcggggg cgacgctcga cgccgtcgcg
    31321 acggtccggc acggccgggg cgagcctcag ccgctcgagg tcgacgcgac ggcgaccctc
    31381 tcctgcgacg gagggacgct cgcggaggcg agcgccggcc tcgtggcgcg gggagacgcg
    31441 aaggtcgacg tcgccgcgac gttcctgccg gactctcccg ggaagagaac ggcggcaggg
    31501 cgcgtccgcg ccgcctctct cgccggcctc gcctccgggc ggatcgaggc aggccgcgtc
    31561 gcgctcgacc tccccgacgc ggcggaggcc gccgcggagc tccgcgcgct cttcccttcg
    31621 ctcgtccccg agccccccga agggctcgac ctcgccggcc cgctgcgcct cgacgccggc
    31681 gtctcgggtc cgctccgggc gccgcgcgcg gcgatcgccg ggacgttccg tccggcgcgc
    31741 ggcggggagc tcgccctgga cgcgtccgcc gacgcggcgc ggggagcggc cgcggggcgc
    31801 ctcaccgccg ccggcctcga cgtcgcgacg ttccacccgg gcgcgacggg gctcgcctcg
    31861 gcagacgtcg ccttcacgct ctccaggaag ggccgacacg ccgacgtcgt cctcgacgcg
    31921 gccgacctct gcctcgccga ggagacgccg cgcgtcgacg cgctccacgc gacgctcgcc
    31981 ctcgacgggc ccgagctctc gatcctccac ctcgcggcca cgagcgccgc agccccgtcg
    32041 ccggccggtc cctcgcccgc gcgcgtcgac gcctccgggc ggctctcgct cgacgcgccg
    32101 ttcgacgacg ccgacctcga gacgctcctt ttcgccgcgg gcctcccggc cgaggcagac
    32161 gccgtcgtcc gtgacggggt cctcttcctc gacgtcccgt cggcggggcg ccccggcctc
    32221 gtcgggacgc tcgccgcgcg cgtcccgctc ggctcgctcc gggagagtcc cgccctcgcc
    32281 ggcgcgattc ccgcggggct ccccgaagga ccgatcgagg tgaccctcga ggctcccggc
    32341 ctcgatgcct gcgcgctcgg gccgctcctc ccgtccggaa cggaagcgct cgcggcgcgc
    32401 ggcgacatgc gcctcttcgc cacgttcggc ctcgccgacc cgctcgcggg gacggccgcg
    32461 atcgagatcg agggcctgac gctcgagtcg cccgccggac cggtccggct ccgcgagacg
    32521 gcgcgcctct cgctcgccgg cggcgagctc gcgcttcagc cggtgacggt cgacggcgag
    32581 aggacctcct tcaccgtcgc cgccgaggcg gagctcctcc ccggcgcacg gctcgggggg
    32641 ccgctcgaga gcctcgtcga acacctgtcg gcgacggctc ggggacgggc cgacgcggct
    32701 ctcctgtcgc cgttcctcca gggcgggatc gcgaagggcg agctcacgct ggacgcgagc
    32761 gcctccggcc cgccccgcgc cctcgcgggg cacgtcctcg tcgacgggag gggagcgcgc
    32821 ttcacctggc cgctcgccta cccgaccgag ctgggcgacc cggtcctcga ggcggagctg
    32881 acgccggggc aggtcgagct gacgcggggc gaggcgctcc tgaacggcgg gccgctccgc
    32941 ctcgagggcg gctggtactc cggcgtcggg gcgaccgcca ccgcccggtt ctccgacgtc
    33001 cgctaccggc tcgcgtacgg gctcgcctcg gtcctctcgg gagagctgac gttcgaccag
    33061 ctcgaggacg agcggaggct ctcggggacc gtcgtcctcg agcgcggcct cctcgagcgg
    33121 gacgtcgacc tcgaccgcga ggtcctcgcg ctcgtcctgg cgccgccgga gtctccgggc
    33181 acggaggctt cgttcctcga cacgctggcc ctcgacctcg ggatcacgac cgcctccggc
    33241 gtgaggatcc ggaacaacgt cgccgatctc accgcttcct ggagccgcat cgacgtgtcg
    33301 ggagcggcga gccggcccgt cgtgaggggg cggatcgacg tcgagagcgg cgggctcgtc
    33361 ttcgcctacg gccagacgtt ccggatcgac cgcggcgtcg tcacctactc gggcgacccg
    33421 cagagcgatc cgcgcctcga cttcctgacg acctcgtccc tcgaggaccc gtcgatcgcg
    33481 gcggccggcg gcgggagcga cctcttcgcg tcagcgcgcc gggacgcctc cggcggagcc
    33541 tcttcggaag acgccgccgc ggcgctcgcc gaggggctcg ccggctacta cgggaaccgg
    33601 ctcgcgggat ctctcggcgc ggcgctcggc cgggtctcgc tcggcgtgcg ccccctcctc
    33661 ctcctcgggg agaccgacgc cgccgcccgt ctcaccctct cgcgcgactt ctcgccgaac
    33721 gtctcgctcg cggtcgggat cgacctgaag aacgcgcagc ggcagacctg ggtcgtcgac
    33781 gtccacggcc tgcggcgcct cccgcctctc tcggtgcagg ccttcacgga ggactacggg
    33841 cgcttcggcg gcacgctcca gcagcgaatc gagctcggcg gcacgcgcga cggcgccggc
    33901 gacgacgtcc ctctcgtcgg cgccatgcgc gcgacgcctc cggccggggt ctcccgccgc
    33961 tccctcgtct cggccctcgg cctgcgcaag ggggagccgg ccgggaagga cgctctcttc
    34021 gaggccgaga tcgacgccga ggcgttcctc cgcggaaagg ggtggcccga cgcgcaggtc
    34081 tcccttcggg ccgtgccgtc gcggaagacc ggccgcgtcg acgtggaggc cgagatcgac
    34141 gccgggccgc gtgccgagct ctccttcgcg ggagaccgcc tcccggccgc cgtgcgccgc
    34201 gaggtcgcct ccctgtaccg gaccgggctc ctcgagcccg cgtcgctcgg cgacatgaga
    34261 cgagccgccg agcgcgccct ccgggcccgg ggtcacctcg ccccggcgat cgaggtcgag
    34321 gcgaccgggc ccgaggagga gcggcgtgtc gtcatacggg tggtcgcggg ccggaaggcc
    34381 gcgatcggcg agcttcggtt cgagggcgtc gacccgcgcg aggccgtgct cctcgggagg
    34441 cgcttcgcga ccccgctcga gcggaccgag ctcgccgccg gcctgccgag cgccgacgcg
    34501 cggctcctcg aggggctccg cgccctcggc tacccgtacg ccgccctcgg ggagcgcgag
    34561 ctcctcgacg gcggacggct cgtcgtccgc gtggagaggg gcgagccgtc cctcgtcgac
    34621 tccgtcgaga tccggggcgt ctcgccggag gagcgcgagc gcttcgcgcg gctcgtccgt
    34681 ctcgcgcccg gcgacgtcgc ggactccgag cggaccgcgt tctcggccct cgcgatcgag
    34741 gaggccctgc gggccgaggg cttcgcggcg gcgcgcgtcc gcgcgacgct ctcccccgcc
    34801 acgccggagg tcccgccgcg cctcgcgctc gcctacgccg tcgagcgcgg cccggcccgg
    34861 cggatcggga acgtgaggct cgagggtctc tcgcgaacct ctccggcgtg ggcgaagagc
    34921 gtcgcggccc tttcccccgg ggacgtcttc cgccgctccg acctcgacgc ggcgcgcgcc
    34981 ggcctcttct ccctcggact cttccggagc gtccgcggcg acctgacgac gggccccgac
    35041 ggccgcgtcg acgtcgtcct cgcggccgag gagctccccc cggtaacgct cgcctacggc
    35101 gtccgctggg agaacgagcg gggcttctcg gccgtcgtcg acgcctcgga ccggaacctc
    35161 ttcggtcgcg gaatcgtcgc cggcgtccgc ggcctctacg accccgagga ccgggccctg
    35221 cgcctcttcg ccggcctgcc ggaacaggtc ctcggagcag ggatcgacct ctgggtcgag
    35281 cggcgacgtt cgttccgcga ggggctcttc tacggccaga ggtccgacgc ggtcgaggct
    35341 tcgctccagc tctcgcgcag cctcgggaag agcctctcgg cgcgcctcta cgggagattc
    35401 aaggagaccc ggtacttcga ggacgacatc ttcttcccga tcgacgtcac gatccgcctc
    35461 ccctacctcg gcgtccagct cgtgcacgac acgcgtgagg acccgatgct cgggacgaac
    35521 ggcgtcctcg cgacgctcga cctgcagtcg agcgggagct ggctcgggtc cagcttcgcg
    35581 ttcggccgcg cctatggcca gctgaacctc taccgtcccg tcctcgcgct cgggagcggg
    35641 aaggtcgtct gggcgcagtc ggtgcgcgcc ggcttcgcgc gcgcgttcga ggggcaggag
    35701 ctgatccccg acgtccgctt ctacgccggc gggtcctact cggtgcgcgg ctacggcacg
    35761 gagagcctcg gcccgcggga ggacctcggc gggacgctct acgtcaccgg cggctcgacg
    35821 ctcctcgtcg tgaacgagga gatccgggtc ccgctccacc cacgcctcct cggcgtcggt
    35881 ttcctcgacg cgggccaggt ctgggaatcg tcccgcgact tcgggaccgg cctcgcgacc
    35941 tccgtcgggc tcggcctgcg ggcgctcacg ccgctcggcg tcctccgcct cgacggggcc
    36001 gtcccgctga agcggcgcga gggagacccc gcctttcgca tcacgttcgg gttcgggaac
    36061 gtcttctgag cggaacctct cgtttcgaga ggcccccttc ccgaaccgat cgccgaaatc
    36121 gcgatttgca cggcctggcg gctggcacgc gtgttgcgct tgtcgagatg ccgcgcgcga
    36181 cgccgcggcg cccgaacaga tccgagaacg gggcacgccc ccgagaagga aaccaggagg
    36241 aacccgtgaa gaagaacctg ctctccgccg ccctcgccac cgtcctcggc ctcgccggag
    36301 cccccgcgct cgccgagacc tggaccgccg acacctccca ctcgaccgtc ggcttctccg
    36361 tccgccacat gatggtgtcg aacgtgaagg gggccttcaa caagttcacc gcgacggtcg
    36421 aggggagccc cgccgacccg gcctccgcga agatcagcgc gacgatcgag gtcgcctccg
    36481 tcgacacccg cgagccgaag cgggacgagc acctccgctc ggccgatttc ttcgacgccg
    36541 cgaagcaccc gcagatgacg ttcgtctcga cgaaggtcga gaaggtctcc gcgacgaagg
    36601 cgaaggtcac cgggaacctc acgctccgcg gcgtgacgaa gccggtcacg ctcgacgtcg
    36661 agtacaccgc ccccgtgaag agcccctggg gccagaccgt catcggcgcg acggccaccg
    36721 ggaagatcga ccggcaggac tacggcgtca gcttctcgaa ggccctggag acgggcggag
    36781 tcctcgtcgg cgacgaggtg acgatcaccc tcgagctcga gctcgtgaag caggaagcga
    36841 agaagtaaac gtcccgagcc gcgtctcgag gcgcccgcgg tcccaccggg gccgcgggcg
    36901 ccttcgcgtc ccgggcgccc ctcagcgcga cgcggcggcg aggagcgcct tcgcgcgctc
    36961 cgcttccggg gccgacgcgg cgatcttcac gaagcggtcg aggtcgagga ccatctggtc
    37021 cttccgcccg agcttctcct gggcgaggcc gcggtagtag tacgcgaggg ccatccccgg
    37081 gtccttcgcg agcgtctgcg tgagcaggtc gaccgcgtcg ccccacttct gctgaaacgc
    37141 ccgcgtcgag cccatctggt agagcgtggc ggcgctcccg ccgtccagct cgcgcgcctt
    37201 cccgagcgcc gtcatcgcct cgtcgaagcg gcgcaggcgg gagagcgcgg cccccttcgc
    37261 gaggaacgcc gcggcgtcgg tcggccgcgc ctccgccgcc ttcgtcgcca gctccgccgc
    37321 cttccggaac gcggcgtcgg cttcggccgt cttgcgggcg cgcaggagcg tctcgccgag
    37381 ccagtgagag gggcgcggat cggccggggc gagggcgacg gccttctgga ggacgtccgc
    37441 cgactcggcg tacttcttct cctgctcgag gaggcggccg agtgcgatgg cgacgtcgac
    37501 gttcgaggcg gcctgggcgg cgaccggcga gagggccgtt cgcgcttcgg cagtcatcca
    37561 ggagtcgagc ttccgctcgg cgccgcgcgc ggcctccaac ggagtcggcg gcgccggagc
    37621 cgcggtcggc gccggggcgg tctgcgcgcc ggcgggcggc gcggcgagga ggaacggggc
    37681 ggcgagggtc agggcgagaa acgaacgtgt catggagcct cctggtaccg ggctgcgctc
    37741 aggatcgcac ggacggcgcc gggacgcgcg ggagacggcc aagcgtctcg cgtcccttca
    37801 gctggccaca ggccgcggca gcgccgagcc ctttcgacca gcggacagtg acgtcgacgc
    37861 ccgaggccgc gagaagcgcg ccgaaggcgt tcaccttctc gtcggcgggg cgtgcgaggc
    37921 cggggagcca ctcgggcgat tcgttgagcg ggatgaggtt caccttcgac gggacccgtc
    37981 gcgcgaggcg cgcgagcgcg cgcgccgcct cgggggagtc gttcacatcg gcgatcagga
    38041 cgacctcgag cgtgacgcgc cggcccggct cgagcggcca ctcctcgagg gcctcgaaga
    38101 gctcgcgaac gggccactgc gcgttgatcg gcatgaggcg ggtgcggagc gcgtcctcgg
    38161 ccgcggtgag cgagacggcc aggttcgggc ggcgctcgcg cccggccagc tcgcggatcc
    38221 ccgggacgat ccccgccgtc gagacggtcg tccgccgcgg cgagacctcc gcctcgagga
    38281 ggtcgagcgc ccgcgagacg ttcgcgacgt tcaggagcgg ctcgcccatc cccatgaaga
    38341 cgacgttcgc cgcgcgcggg ccgaagccct tctcgcgggc gacgacgagg tactgaccga
    38401 ggatctcgcc ggccgagagg ttccgcccgg ctcccatccg ccccgtcacg cagaacgcgc
    38461 aggtcagggc gcagcccgcc tgcgacgaca cgcagacggt gacgcgctcg gtcgtcccgg
    38521 agttccggcg gcgcgacgcg ggcaggaaca cggcctccac cttcgctccg tccgcgaggc
    38581 ggaagaggaa cttctccgcg ccgtcgtcgg acggcgtccg cgcctcgacc tccggaaggc
    38641 cgacgacgaa ccgctccccg agcgccgcgc ggagcggggc ggggaggtcg gtcatctcgt
    38701 cgaacgagcg ggcgcgccgc tccgtcatcc agcgctgcac ctgcgccgcg cgaaagcgct
    38761 ctcgcgcgag cggggcgagc gcctgcgcga gctcggggag cgtgagcccg aggagcgggg
    38821 tcttcacgtc ggatccgcgg gagactgggg cgatcgcagg cgccgaggcg ctctcatgcc
    38881 tcagacccgt ttcgagacgg tcgtccggag cgagacggcc gtcccggtcc ggcgccgccc
    38941 gcgcggcgcg cgcggggcgc gcgtcgggtc gatcccgagg gagcgcagga agtggaggtc
    39001 cttcacgtcg atcttgaagg ctgccccctc cgcttcctcg cggccggcgg gtccaccgcg
    39061 gcggatcgac gggagcgccg cgcgggcacg gcgcccccgg gcgtcgaggg cgacggtcgc
    39121 gtcgaggacg aggtacggct cgtgcccctc ctcgcgaagg ctctggagga tgccgtgcac
    39181 ttccgccgag gaggagacgg cctcgttcag ggccttcccc agctggcgga gcgccgcgcg
    39241 ggtcttctcg tcgagcgcca tcgactcgac tctagagcgg ccgaacggcg cgcgtcaatc
    39301 ttcgccgcgg tcgaatcggc ggtcggcgag ccgcgcgagc acggcgccca ccttgcccgg
    39361 cgtcgagagc gagatgcggc ggcgcagcgt gtcgagcgga cgcgcgagga ccgcccgcgc
    39421 cacgtcggcg tagatgccgc ccatcatcgt caccgcgaag cggcactcgc ggtcgacgag
    39481 ctccttcacc ggctcggccc tccggaagta ggagagcgcg cggcgcgcct cgaagcggac
    39541 gagctcggcg gcggcgggcg tggcgcggag cgacacgacg tccgcctccg tcacgccgaa
    39601 gcgcgcgagg tcctcgagcg gcagatagag ccgcccgcgg cccacgtcct cgccgacgtc
    39661 cctcacgacg ttcgtcagct ggagcgccgt cgcgaggtcg cgcccgcgcg cctcggcctc
    39721 cgcgttcccg aggccgccga agatcgcgag cgagatcgtc gagatcgtcg aggccacgag
    39781 gtcgcagtag ccgaggagct cgtcgaacgt cgcgtagcgc gtcttcacga ggtcctgccg
    39841 gcagccggcc aggagccctt ggaacgccgt cttcgggatc gggaagcgcg ggagagagcc
    39901 ggcgagcgcg atccccgcgg cgctcgtcgg cgtcccgccg tagaccgcgt cgagctcctc
    39961 ctcccacgcg tcgagccgcg cgggggcgcc ggccgggtcc ccctcgtcga cggcgtcgtc
    40021 ggcgacgcgg caccaggcgt aggcggcgta gaccgcgtga cggcgttcct tcgggaggaa
    40081 gcggaatccg acggagaagt tcgcgccggc tttgtgcgtg agcgcccggt tctcgcgatg
    40141 cgccgaggcg agcgcggcgc tctccgtcac ccgatcctcc cgagcgcgac ctcgcggatc
    40201 tcccgctcgc gcttccggca ccaatgctcg ggaagcctgt gcgcgcagcg gcctctgttc
    40261 cgccagagcg ccgtcacgat cggcgcgaga gcgaggcgga gagcgccggg gcggtagaac
    40321 ccggcgagga cggagcgaaa gtcggcgtgc gcggcgaggc gcgcgaaaag cgagaggccg
    40381 aaggcgccgc cggcctcgta gaggaagcgg ttcgcgacgg agacgcgctg gcggcgggcg
    40441 aacctcgcgc gcgcgagagc cgcgaagtcg cccccggcga ggagcgcctc ggccgcgaga
    40501 gcgcctgagg agatcgcgta gcgcatcccg aggccgaaga ggtagtcctg gaacgccccg
    40561 gcctcgccga cggcgggacg tccttcacgc gacgcctcgg gcgcgagggc gaagttcatg
    40621 aacgagtagg cgtcgcgcgc cccctccatc gggacgctct cgacggagag gagcttcgcc
    40681 ttggccctct cgaagtagcg gtcgatgccg ccgaggtctc ggacgatcgc gcagccgaac
    40741 gtcccgcgcc cgccgacggt gaagagatac gcatagcctc ccggcgcgac cgaagggtcg
    40801 aagaggaccc ggacgcgcgt gacgccgtcg ctctcgaagg ccgtctctcg ggcgaggccg
    40861 tcgggtgtct gcggacccgt cgccacgagg tccgcgtcct tcgcctcgag gcgcgagcgg
    40921 aagcggacct cggccccagc cgcgagggcg cccgcgagga gcgtcgtgtc gagcgtcccc
    40981 tcgcccgggc cgcgcgtcac gaaccaggcg taaggctcgc gcgacgacac ggcgaagcgc
    41041 cgcagcttcg cgtcgaagaa ttctgccgtc gtcgcgggga cccaggccgg cgccgggagg
    41101 ccgaggcgcg agaagagcgc cgggaccgtc tcgcgcgggt ccgaggcgtc ctcgatcacc
    41161 tgcaggtcgc cgacgaagcg ggcgccgacg gtggcgttcc gctcgtggac gacgaccctc
    41221 cgcccggccc gcgcgaggag ggtggcggcg gtcaggccgg cggggccggc tccggcgatg
    41281 ggaaggtgcg ggaggtcggg cacgcgagga gcgattatcc cggttcgccg ggagggtgac
    41341 ccgcgcggcg atctcacgat gccctccccc ggaacctggg ggccgctacc cacgggaggc
    41401 gcgctcccgt cgagtccgaa tccgtcgagc gaccggcgcc agagacgtcc tcgcaacgtc
    41461 ccggactaaa ctccggccct ctcggaaagg cctccagatg cgcttcgact cccttcgccc
    41521 ccgactcgag cccttcctcc tccgcgtcga gcgtcccggc cgctacctcg gcctcgagcg
    41581 gaacgtcgtc cggaaggagc tctccgccgc ggagctgacc ctctgcctcg ccttccccga
    41641 cacgtacgag atcgggatga gccacaccgg cacgaaggtc ctctacgagc tcgtcaaccg
    41701 ccgcgacgga tgggcctgcg agcgcgccta cgccccgtgg gtcgacctcg aggaggtgat
    41761 gcggcgcgag cggatcccgc tcttctcgat cgagtcgtgc gccccggtcg cggacttcga
    41821 cgtcctcggc ttctccctcc agagcgagct gaactacacg aacgtcccga acatgctcga
    41881 cctcgccggg gtcccggtcc tcgcggccga ccgcggcgac gccgacccgg tcgtcgtcgg
    41941 cggcggcccg tgcgtcgcga accccgagcc ggtcgccgac ttcttcgacg ccttcctcct
    42001 cggcgacgcc gaggaagcgc tcgccgtctt cctcgaggcg gtccgggcga cgcggggcct
    42061 gccgcgccgg gaacgtctcc tcgcgttcgc ggcctgcccc gggatctacg tcccctcgct
    42121 ctacgacgtg agctacgacg gcgaccgggt cgtcgcgtac acgccgaacg ccccgggcgt
    42181 cccggagcgg gcgaagcggg tctgggtcga gcggctcgac ccgtccgtct accccgaaag
    42241 gccgatggtc ccctcggtcg acatcgtcca ggaccgcctc ggcctcgaga tcatgcgggg
    42301 ctgcacgcag gggtgccgct tctgccaggc gggctactgg tatcgccccg tgcgggagct
    42361 cgacccggcc gacgtcgccc ggatgacgaa ggcgttcatc gacgaggcgg ggtggtcgga
    42421 ggtcgggctc ctgtccctct cctcggccga ctactcgcag atcgagcccc tggtcgcctg
    42481 cctcgcgccg gagctcgcga agacgaaggt ctcgatctcg ctcccctcgc ttcgcgccga
    42541 ggccttctcc gtcgcgctcg ccgacgccgt ctccgaggtg cggaagtcgg gcttcacgtt
    42601 cgcccccgag accggctcgg accgcctccg ccgcgtcatc aacaagacct tcacgaacga
    42661 cgacatggtc aaggccgcgg gggtcgcgtt cgagcgcggc tgggacctca tcaaggtcta
    42721 cacgatgatc ggcctcccgt ccgagacgac ggccgacctc gacgagctcg tgacgctcgt
    42781 ggcgaacatc ctcgccgagg gacggaagaa gggcgtgcgg gccacggtcc acgtctccat
    42841 cggctcctac gtcccgaaga gcttcacgcc gttccagtgg tccgcgttcg acggcgtgga
    42901 gcggctcacg gagaagctcg cgtacctgaa ggagaggttc cgccccgtcc gcggagcgaa
    42961 gctgaagtgg cacgagccga aggaagccga gatcgaggcg atgctctcgc tcggcgaccg
    43021 gcggatgtcg ggcgcgctgc tcgaggtctg gaagaggggc gggaagttcg acggctggtc
    43081 cgagcacttc tcctggccgc gctggaccga ggcgctcgac gcggcgggaa ttccgaaggc
    43141 gcgccacctc cgcgagaagg acctgaagga gacgctcccc tgggacgtcg tcgacgcctc
    43201 catccgcaag cccttcctcg tcgtcgagtg gaagaaggcc ctgcaggaga tggcgacgga
    43261 cgactgcaag tggggccggt gcacggcctg cggcgtcccc ggcaacgggg aggacaccgt
    43321 cctcgcgaac gggatgccgg ccgcgttctc gttcgcgctg ccgaccctcg gagcggcgga
    43381 ggccggagcg ccgccggcgc cggttccggg ggacgccggc gacggcgggc gcgcggccgt
    43441 cgagatctcc gctcttccct ccgcatccgg cgtcgcgagc ggcgtcgccg acgcccgcga
    43501 cgaggggcgc ggcgcggcct acgccgacaa ggcgaagggc gccgcctatc ggatgaaggc
    43561 gacacccgac cttctcccgc tccgcgagcg gcggacctcc cggggcggag cgagcgagag
    43621 cgccgcgatc gcgaggagct ggcgcgtctc cttcgagaag ctcggcgacg cccgctacct
    43681 ctcgcaccgg aacgtcatgg acgtcctcga aagggcgctc cgcgccgcgc gcgttccggt
    43741 ccggtacacc gagggcttca acccccacat ccggctctcg atgggcccgg ccctcccgct
    43801 cggctccgag tcgcgccacg agctcttcga cctcgacgtc gtcgagcctc tgacgctcga
    43861 gcacctcgcc gccgtcaacg accgcctccc gcccggcatg cggctcctcg gctggacgga
    43921 gctgccgccc ggcgcaccgt ccctcgggaa ggcggcgacg gaaggggtct accgcttcac
    43981 gctgccaaac ggcgaagtga tcgtcgagcg tctgcgcctg gcgggcgagg gggcgacgac
    44041 gccgaagcgc cacatcgaga agacgtgggg cctcgccgcg gaggaccagc acgtcatccg
    44101 tgtcgttcgc gaggagaccg tcctgacgcg ctgagtgcgc gacggggaga acgcgtcatg
    44161 cccttcacca tccgctgcca cgcgacgttc gaggccgccc accacctccg cgactacgtc
    44221 ggagggccgg agcccgtgca cgggcactcc tggaaggtcg aggtcgccct cgggaccgac
    44281 ggtctgggct cgtacgacct ctccgtcgac ttcgtcccga ccgagcggct cgtccgcgag
    44341 ctcgccgcgc gcctccacga ccgcgacatc aacaccgtcc ctcctttcga cgtgaagaac
    44401 ccgaccgccg agaacgtcgc gctgtggttc gcggacgagg tgcggaaggc gggcgtcctt
    44461 tcggacggcg tccgcctcga ggaggtgatg gtctgggagg ggccgaggaa ctcggtgacc
    44521 taccgaccct gagcgggtgc gcacgagtca tccttccgaa gagtctcgag taccgatcgc
    44581 gacgtcgccg aacgaggacc tcttccgtcc ggattctcgg gagtgcgcgt cgggagtgcg
    44641 cggctcggtc cggaaccgcg caccgcgtga gccccgacac ttgccaggtc gccgaagcga
    44701 cgacagattc gctccagacc cacatcggaa cctcgcgaag ccgcgcggag ctccgtcccg
    44761 caacgtcccg ccgggcggga ctggcagaag gagcgaacca tgtcctgtcg atccatcgga
    44821 gccctgttcg ggctcctgct tctcgttgcg cctcgcatct cggccgacga agtgcccctc
    44881 cgcaactgga ccgtcccgtc ctcgcccacc cggctctccg cactcggcga catcggcaat
    44941 cccggtgtct tcgtcgcggt cacgccgtgc cgcgtggtgg acacgcggct cgggatgggt
    45001 ttcagcggcg cctacggtcc gccggctctg acggccgccc cccgtacgtt caacatcgtc
    45061 gcgtcgggct gcaccggcct tcccgcaaac gtctccgcgt tctccttgaa cttcgctgtg
    45121 acgaacacgg ccgggcccgg ccacatcgtc gcgtggcccg cgggcggcac gatgccgaac
    45181 gtgtccacgc tcaactacct cggcgcgcag acgctcgcga acgccgcgat cgttcccgcc
    45241 ggctccggga cgtccatctc ggtcgtcgcc gccgtgagcg ccacagacct gatcatcgac
    45301 atcaacggct acttcacgaa ctacccgaac acgaacaagc agctcttcac ctccgggtcc
    45361 ttcagcgcgg ccgcagcaat cctcggcgtc aacgactcga acgcgatcgg ctcccatggc
    45421 gtcgggggat acgccggcgg gacgggagtc gtccacggcg tgcaggggga gatcggatcc
    45481 ggggccgccg acctgtcggc gggcgtccac ggcatcggcg gcgccggcac ggcacggact
    45541 ttcggcgtgt acggcgaatc ggcaacgaac gcgttcgacg cggccggcgt cctcggccgt
    45601 tcgaccttcg gcacgtcgcc gatcatcaac agcatcgcga atacggcggg ggtacgggga
    45661 gaaggcgccg ccagaggcac cgtcggggtc gcggggccct ccggggtcgg ggtccggggc
    45721 tactccgtcg acgcgtccgg catcctccag acgttcggca gcctcggcca ggggaactcc
    45781 tacggcgtct acgcgaacgg ggacctcggt gcgacgggca cgaagacgtt cgtcgagccg
    45841 cacccgaccg acgcttcgaa ggtgatcaag ttcgtctcgc tcgaagggcc ggaggccggg
    45901 acgtacttcc gcggcaccgc gtccactcgc ggaggtgtct gcgtgatcga ggtgccggag
    45961 ccgttccgga tggtcacgga cgaggaaggg ctcacggtcc agctgacgac cgtgggctcc
    46021 ggggcccacg cctgggtcga gagcgaatcg ctcgagagga tcgtcgtccg ctccagcgcc
    46081 gacacgaagc tccattacct cgtccagggc gtccggaagg cctaccgcga tttccagcca
    46141 gtcgtccccg gcgacgagtt catgccgagg tcgcccgagg acacgctgcc gctctggctc
    46201 tcggaggaag gccggcggcg gctcgtgtcg aacggcacgt tccacgcgga cggcaccgtg
    46261 aacatggaga ccgccgagcg ggccgggtgg gcgcagcgct ggcgcgaggc cgagagggtc
    46321 cccgagtagc gggtctgatc acgaggggcg aggcaccggc gccctcgagg cgcgggggcc
    46381 gcggagccgc ggcccccgtc ctcgccgcta tctccccgcg gccgtgccga acgacgggag
    46441 cgccatgtcg acgcccgcct tcgcggcctc gggcagctcc tcgggcgcga ccgaggggcg
    46501 cggccggtag gtcggcagag ggaccgactt gccgcccgcg tagatcgggt ggcgcccctt
    46561 gtcgcggtac cagtcggcga gcgtggccgt ctggtgcatc tcctcgatga tctgcctcca
    46621 gccggcgccc gtgttgtagg cgcagaagga gatctctccg agctgcgtcc cgtacgggat
    46681 gatgcacatc tccgtccggc ggaagtcgta gttgaagagg tcctggaacc acataccggc
    46741 gacgaggagg acgcgccagg ggtagcggcc cgacttcgcg atccccatcg acttgccggt
    46801 gtggccgtcc atgatccgga gcgtctcgag gacgccgagg ttcctcggca cgcccgcggg
    46861 gcggatgttg cgcgccgtgg cgaggaccgc ctggaggacg gtgaggaagc gcccccgcgc
    46921 ggcgtccgtg atgacggtca ggtcgcgcag gagccggtcg acgtcgagga gctgcgtcac
    46981 ggggaccgcg tcgcccgtct tctcgtggac gaggaccatc gtcgcgatgc cgcagttcgg
    47041 gtggcagccg cacttcagcg agccccactc ggcctccagc cctccgagaa ggtcgcggag
    47101 gtcggagaac ggcccggacg ccgagagcgg gaaccagtcc cgcatcggct cgcccaggcc
    47161 cgcctggccc ttcacgtcgt tggccaggtg cgagagcgtg tagcgctgcc tcttccgggt
    47221 ctcgtcgtcg atctcctcgt cccttccggt gaacgagacc ggctggaagg agagggcgtt
    47281 gatcttgtcg atgttcttga tcgcgaaccg gatgatgtcg ccgacctggt cgttgttgat
    47341 cgagttgacg atcgtgatga cgagcgtcac gtcgacgccc gcggccttca ggttctcgag
    47401 cgcgcgcagc ttgacgtcgt agaggttcga gacgccccgg tgctggttgt gctcgttgcc
    47461 gaccccgtcg aactggaggt agacgaggcg gagccccgcc tcggccgcgg ccttggcgaa
    47521 gccgggctcc tgcgcgaagc ggacgccgtt cgacgcgcac tggacggcga agtagccgat
    47581 ctccttcgag tagcggatcg cctcgaggaa gtggggcgag agcgtcggct cgccgcccga
    47641 gaactggacg gagagctggc gccgcggctg gatcgacgcg gcgttgtcga ggatctgctt
    47701 cacgtcgtcc caggtcagct cgtggacgta gccgacctgg ttcgcgtcca tgaagcaggg
    47761 gttgcacatc atgttgcagc ggttcgtcag gtccaccgtc aggaccgccc cgcggccgta
    47821 gcgcatcgag gacgagccgt ggtcgtggag cgcgtccttg ccgatcctca cgtcgcgccc
    47881 gaagaagttc ccctcgaggc gccggtagaa gtcgacgtcg ttcgacatca ggtcctcgaa
    47941 cgtgccgtgc ttcgggcacg tcttgaccat ccagatctcc tggccgcgct ggacgatgtc
    48001 ggccgggatc tcgccggggt ggccgtcgat cagggctttc aggtcctcct ggcccttcac
    48061 gatggcctcg cgaacttcac gtgtgcacac ggggcagagg gagtccgtcc gccgcgggaa
    48121 gccgagcggc ggcttcgtgc gctcgtggct cttcggcaac ggctcgggcg accacgccgg
    48181 gtggaaggag cgggccggga ccttccggtt gacccactgg aaccccttcc aggcggcgtc
    48241 cgcggcgcct ctcaccgtcg agtagaaggc ctcacgcatc atttctcggg tcctccctga
    48301 agggcgacgc ccgcccgctc cgcaggagcg gctcgccacc tcgaatactt agccgagcag
    48361 aataccattc gtgcccgctc agagcccgaa acgcgccgga gcgaagggag cggccggggc
    48421 cggcggctcg tggcgggtga cgaggtcggc gacgaatcgc gccgcggcgg gcgagaaagc
    48481 gagctcgtcg ctgccccagg ccgtcgccag gaagaggccc gcgacgtccg tctctcccgc
    48541 caggggaagg ccgtccggcg tccggagctc gggcgcggcc ccggactcgc ccgcctccca
    48601 ggccatcgag gccggaacga gctgtcgcag cgcctccagg aggatcgcga ggcggctcgc
    48661 cgagcaccgc ctcgtccgtc cgtcgcgccc gggccggccg agcgcgacga gcgtcccgtc
    48721 ccggcggggg acgaggcgga cgccgctcga gacgagaagg cggccggggc gatccgggtc
    48781 cgaggacgcg tcgagccgga gccacggccg cgaggccgcc tcgagcggga acggcgggag
    48841 cccggcttcg ccgcgctcga ccgcctcgcc ctccgcgagg acgaccgccg cggacgggag
    48901 gaagccggcc ggcgtctcga cccccgagac ccggccgccg gtccgcgcga cgacgcgggc
    48961 gcccgtgccg tcgatcaggc ggactccggc gctccgcgcg gccaggacga gggcctttcc
    49021 gacgcgacgc gccctcgcca cgccgtcccg cgggaaggag aaggcggccc ggagctccgg
    49081 cgtgagcgcc ggctcgcgcc ggagcgcctc ctccggctcg aggacctcga acgggacccc
    49141 gcgcctccgt tgcgcgtcga gcgcgcggtc gaggaggacc tcttccggct cgtcgaacgc
    49201 cacggtcagg gcgccccgct cgtcgagctc gcacgggacg ccggtctcct cctcgagatc
    49261 cgagagccag tcgggaaaga ggtcgcgcgc gaggtgcgcc agccgcgccc gcgcctccgg
    49321 gaccgcctcc tccacgaagg cctgggccgc gaccggggcg ccgggaccgc gcgggcggcc
    49381 gaatcgcccg gagccgagga ggacgacgtc gaggtcacgc cgcgcgagct cgagggcgac
    49441 ggcggccgcc gcgagcccgg tgccgcagac gaccgcgtcc gcggccgaga gcgtccgcgt
    49501 cacgcgaccc ggccctccgc ggtgaggacg gggccccggc ccgcgatctc gggcgggccc
    49561 caccgccgct ccacacgcgc gacgacggcc cgcccgacgg cccggaccgc gatgtagttc
    49621 agggccgccg aggcggcgat cccggcgacc gggatcaggt gcgcgagggg acgaccggcg
    49681 gcccggacgg cgagccgcgt cgcgagcgac tccgccactc cgaccgtgag gcgccggccg
    49741 accgagaggc cggcgccgga ggcgaggccg gcgacgacct cgagggcgcg ctcctccggg
    49801 tcgaggtcgg ccccgtagag aaggtgaagg ccgacgatca tccggctctg gaggacgatc
    49861 agggtcgtca gctcgggcgc cgtcgcggcg acggccgccc accccggggg gacgctcgcc
    49921 gccgcgccga cgagcgcggc cctccgggcc gtcgactcga ccatgagcgc cgccttccgc
    49981 cgcgtcgaga gtccgggatg ccgtcgcgag agctccagga ctccgtcgcg gatcctctcc
    50041 acgtcgaggt ccgtcgaggc gcgccggacg agggacagga acgactcggc gatcttcacg
    50101 tcggctccgt cgaagtatca tcccccccgc cctgccgccg gaacaaggcg gcgccggcga
    50161 cgtccgccgg acgaagaagg aggttccgtg cacgcgatcc gacccatccg tttcgccctc
    50221 gccctctcgc tgggtctcgc tctcgcggcg accgccttcc cgtccgacgc ggccgcgcct
    50281 cagaagaagg gcgcgtcgcc ccggaaggcc gcgccgaaga aggagaagaa ggtgtcgtcc
    50341 accccgcgcg actacgcgaa gaccctcgcc gtcctgaaga cctcccaggg agacgtcacg
    50401 atccggttct tcttcgacaa ggccccgggc cacgtgaaga acttcatcga cctggccgcg
    50461 cgcggcttct acgacggcac gctcttccac cgcgtcatcc cgggcttcat ggtccagggc
    50521 ggcgacccgc tcacgaagga cccggccaag agctccctgt tcggcaccgg cgggaacacc
    50581 gacaaggcgg gcaagccggt gaacgtcaag gccgagttca acgacgtcag ccacaagcgg
    50641 ggcgtcgtct cgatggcccg agcgagcgac ccgaactcgg cctcgtccca gttcttcatc
    50701 gtcgttcagg actcgccgtt cctcgaccac cagtacaccg cgttcggcga ggtcgtgaag
    50761 gggctcgagg tcctcgacaa gctcgtgacg gagtcgaact ccgacacctc cgacccgcga
    50821 agcggcggca agccccgcgc ctaccagaaa gtcctgaagg tcgagctcgc cgccgaggcg
    50881 gcgcccggca acgcgtgacg tctcccggcc ccgccgccct ggaggcgcgc gtctccgcgg
    50941 catgctcccg gttcggcctt ccggaatccg gcccccttca gctcctggcg ggcgacgtcg
    51001 gccagcggag atacgtccgc ctccggacct gggccgacgc gacggtcgtc ctcgtcctct
    51061 accccgagcc ggcttcgccg gcgcaggaga ggtggcgccg gatcggcgag gccctcggcc
    51121 gcgagggcct tcgcgtcccg gcgctcctcg acgacgatcc cgcctcgggg acggcgctcg
    51181 tcgaggacct cggagaccgt gacctggcgg ccgagatccg ggaggcggat cccggcgagc
    51241 gcggacgcct gatcgaggag gccgaggagc tcctcgcccc gctccgcgcg atgtcgcgcg
    51301 aggccgcgct cctcaacccg cccttcgacg ccgcgttctt cgtcgcggag ctcgcgcaca
    51361 cgcggcgctg ggcgctggag agcgacggcg cgcgagcgct ctccccggag cgatccgccg
    51421 cgtgggagga gctcgcggcg agcctcgcgc gcgaggcggc ggacgcttcg cgagccggcg
    51481 gccccgtccc gacgcaccgc gacttccacg cgaacaacct gatgcgcacg ccggaggggg
    51541 ccctcgccct gatcgacttc caggaccttc gcctcggcca tccggactac gaccccgtct
    51601 cccttcgatt cgagcgcgcc ggggcgctcg tcccgtcgag cggggacgac cggagcgagg
    51661 ccgtcctcct ccagcgtgcc tggaaggtcc tcgggacgtt cgagaagatg ctcgcgctcg
    51721 gacgggaggt ctatcggccc caccgggaca ccgcgctcct cacgatccgc gcgcggacgc
    51781 gccccgacgg cgcctgggca ccgctcctcg acttcctgcc cgcctgaacg ggtatcattc
    51841 ccgccctcag gaggcctacg tgcccaacct cggactcccg gaacttctga tcctcctctt
    51901 catcgtcgtc ctcctcttcg gcgtcgggaa gctcccgcag ctcgggaagg ggatcggcga
    51961 ggggatccgc aacttcaagc aggccgtcaa ggacggcggg caggactccg agaagaagga
    52021 gcccccgacg ggcgacggct ccgggaacgg ctcctccact ccgaagtgac ccccggcctc
    52081 cgccgggcgc cggaatagcg ggccgcggtc cggcgttttc tccgccggac gggtgcgcac
    52141 gccccctgac cggcacggcc cgggcggttc gcccccctcc gcgggagtcg tcagcatgaa
    52201 gatcgccaag cgcgtctttc tctttctcgc cgtcaacgtc ctcgtcgtcg cgacgatctc
    52261 ggtcgtcctg agcctcctcg gcgtccggcc gtacctgaac gcgtacggcc tcgacctgcg
    52321 ggccctcgcc gtcttctgcc tcgtctgggg cttcggcggc gccttcatct cgctcgcgct
    52381 ctcgcgggtg atggcgaagt ggggaatggg cgtcaagatc gtcgacccga cgacgcccga
    52441 cccgggcgcc caggagctcg tctctaccgt tcaccgcctc gcccaggccg cgggcctgac
    52501 gaagatgccg caggtcggcg tctacgactc ggaggaggtc aacgccttcg cgaccgggcc
    52561 gacgaagaac cgcgcgctcg tcgccgtctc gacgggcctc ctccggcgga tgcggcggga
    52621 ggaggtcgaa ggggtcctcg gccacgagat cgcccacgtc gcgaacggcg acatggtgac
    52681 gatgacgctc gtgcagggcg tcgtgaacgc gttcacgatg ttcctcgccc gggtcctcgc
    52741 gttcttcgtg gcgcagttct tccgccgcgg agacgaggag gggggtggcg tctcgcacct
    52801 cgtctatttc atcgcgacgc tcgtgttcga catcgcgttc tcgatcctcg gctccttcgt
    52861 cgtcgcctgg ttctcgcggc tgcgcgagtt ccgcgccgac gccggcgggg cgcggatcgc
    52921 cggccgcgac cggatgatcg gcgccctcga ggcgctccag cgcacctacg gcatcgtcga
    52981 cccgtcggac cggcaggccg ccgtccagac gatgaagatc tcgggccacc cgaaaggcct
    53041 catgcgcttc ctctcgacgc acccgcccct cgagacgcgg atcgcccggc tcaggatgct
    53101 cgcgtagcgg ccggttcgtc ccgagtcgcc cgagaggccg gcgcgtcgcg ccggcctctc
    53161 ttcgtctgcg gcgcccgccg cgcgggcgga gacgactcgg gggaggcggt gcgcctcccc
    53221 cctcccgttc tcgtcctggc tatgtatccg gcggccccgg ctccggccgg ggcgctcgcc
    53281 tcacgcgtgg tcccggcgct tcggggcggg cttcagacgg agcagcgaga tcttcgcgcg
    53341 gagcgtgttc cggtggatcc cgagggactt cgcggcctgg cccatgttcc cggaagcccg
    53401 ggtcacagcc gtggcgacgt acttcctctc gaactccttc ctcgccgtct cgagggggat
    53461 ccccttggaa acgagctcgt cgatgatccg ctggagctgt ccgtcgagca tcacggtgac
    53521 ctcatcctcc tcgtcgcaaa ggaacccgcg tcgggcgaaa gggacggcaa cttatcacgc
    53581 gacggtgcgg agcggaacct cgcggtctca ctcgcggcgc ggcctgcggg cggccgcgcg
    53641 ttctcccgct acttcagctc gacgaagtca ccggcctgca tggagtcgac catcgacgtg
    53701 atcttcgcga cgcaggtctt tcccttcgtc cagaggacgg cggcttcgcc gaggagagtc
    53761 cggaccgtgc cgctgtcgtt ccgtttgcgg aagacggtca ggaaatctcc cgggtaaagc
    53821 ccgtcgtcct cgccgaggtc gaggaagacg accgagtcgg ttccgacggg cgtcgcgcgg
    53881 tccttcacct cgacgatccg gcccgtcacc ttcccggacg gagggtcgca ctgcgtgaga
    53941 ggagcgctcg cgcggacgag cgggatcggg atcggctcga acggaatcag gaggtcgccg
    54001 aggtaggtcg gctcgcacga ctcggtgatc tcgaggatcg cggagtcgtc ctggacgcag
    54061 acgacccgcg cgcgtgcggg cgtctcgtag aaccgcccga tcgtcgcggt gtaggagccc
    54121 acctggtaga cctcgtgcgc cggccggatc acctggaact cctgacccgc catcaggccg
    54181 gcgcgggcgc cgacgtccga gtagacgacg tcgccgaccc cgaacatctg ccggctgtcc
    54241 tcgtattccg cggcgacgat ccgacccgtg accggctcct cgtcgacccc gatccagccg
    54301 gcacagtaga cgtcgtcttc ccagccgacc gggaacggcg ccgacgtgga cgtcctctcg
    54361 gtgggcgcct gctcgatcac cgcgggcgcc tccacggcct cctgcggcat cggcaccggc
    54421 gcggcctccg ggaggcgcga cggcgaagcg gctgcggcgg agagagagat gacggcacag
    54481 gcgaggacga ccatcgcggg aacgaacacg cgcttcacgg ctccgacctc ctcgaggacg
    54541 ccgcggtgcg accgcgcccg acggcctcgg ctcgattctt ccggactatc gacgctaagt
    54601 taccgcaacg tcgacgtttc tcaaagcccc gtcgaggccg tggccgcgac gggggtctcg
    54661 gggaggcggg ggactttccg gaggaaacgg ctctcggggt ggtcccggcg gagccggtcg
    54721 aacaggaccc gggcgtcctc ggtccggccg agccggcgct cggtctgccc ggcccagtag
    54781 agcgccttct ccatccccga gtattcgggg aaggccgcga aaagcccctc gaagcggctc
    54841 cgggcggcct gccaggcgcc tcgccggtag tagaaccgcg cgacgaggaa ctcgtgctcg
    54901 gcgaggaggt cgagcatcgc cttgtagcgt gcgcgcgcct cgaaggcata gggagagctc
    54961 gggtagctca cgacgaggtc gctgtagctc tgggccgcga gccgggtgtt cgcctgatcg
    55021 cgatcgggct tctcggcctg gcggtcggag acctgcgcga gccggaagag cgcatagtcc
    55081 gaccgcgggt gcgacgggta acggctccgg aagtccttga accgggcctg cgcctcgacg
    55141 tatccggtgg gcgacttctc ctccaggaac gagtcggcga gccgcagcgc cgcctgcttg
    55201 ccgagcgggt cgttcgcgta gttctccgcc acgaaccgga ggtactgccg cccgacctcg
    55261 aacttccgct tcgtcacgag ggcgtctccc tgcgcgtagg cgtcctcctt cgacatcgtc
    55321 agcagctccc gcgtcacacg cgacgggtcg ctcgaggagc aggctcctcc tccgaggacg
    55381 gcgaggccga ggacgaggga cgcggcggtc ttggcatgag tgcgatgggc cattctcttc
    55441 tccggattca gcccggctgg ggctcgggcg aggcgacgac gaaggacagg gtgtcgcgga
    55501 ggccgacggg ggcgccggcg agcgggaact gccgcaggat cgtgccggcg gccgctccct
    55561 cgtagggctg ggccttcacg cccccgagcc ggaagccgcg cgcctcgaag gcgagacgga
    55621 cccgttcgaa gtcgcggccg atcaggtcgg gcatgacgta gaggacatcg ggggcgccgc
    55681 ggttgacgag gacgtcgacg ggcgtctccg gctccgtggg cgctcccggc acgactccct
    55741 gcgcgacgac gccctgcggg ccgggcatcc gcgcggccga gaccgccccc tcgacgaggc
    55801 cttcgcgggc gagagcgagg gcggccgtcc gggccgtcag gccggcgaga tcgggcgcct
    55861 ggatgacccg cgggccgagg gagaggaaga cccggagatc ctgcccggcc ttgacggcca
    55921 ttccggccgc cgggctctgt ccgcgcaccc ggtgcgccgg aatctcctcg tcgaatccct
    55981 cctgcgtggc gtcgacgacg acacggagcc cccgctcggc ggcgagcgcc gtcgcttcgt
    56041 cgaccgacct ccccgtcaga tcggggacct cctccgcctt tccgaggacg aatcgggaga
    56101 acgagatcca cgcggccgcc ccgaggatcc cggcgaggag gagggcgtag aggatccgtg
    56161 ccagaacgcc gctcattcgc gcggacttta gcagggggcg tgccttgacc ccgattcccg
    56221 ggacgaggag actccgtctc catggccgag acccctcgag tgacgagatc ccgcggcccg
    56281 gcggcgacgc cggagctccc gctttcccgc gcggacctcc acgtccacac caacgcttcc
    56341 attttcaagt acttcaaggc ggccaacagc cacgattcct acaacgatcc ggaggaggtc
    56401 taccgcctcg cgaaggcccg cgggatggat ttcgtcacct tctccgacca cgacaccgtg
    56461 gaggggtgcc tgaggttcca ggaccgccac cccgacctga ccgacttttt cgtctccgtg
    56521 gaggtcgaga cctggttccc gaagacgggc catcggatcc acgtgaacgt cttcgacctg
    56581 acccgccagc agcatgccgt catcgaccgg aagcggaaga gcatcttcga cctcgtcgag
    56641 tacctccggg ccgaggacct cctcttctcg gcgaaccacc tcttccagtc gtaccggatg
    56701 cgtcaggccc ccgaggtctt cttcgagacg atgctctccc tcttcgacgt cttcgaggtg
    56761 aagaacgggt cgatggccta ccagcacaac gcgctcgtcg aggacctcct gctcgtggcg
    56821 aaggagaaga gggggagcct cgccctcgtc gggggctccg acgcgcacac ctaccctccg
    56881 atcgcctccg tcttcacgat ggccccgggg aagacctgga aggagttcct ggcctcgatc
    56941 cgtcgcggcg aatgcctttc ctggggctcg gagatgggct tcacgaacgt cctcggcgac
    57001 gtctaccgga gcatcgggaa gcactaccgg gaggtgacga agctccgcaa cccggactac
    57061 acgccccgcg agaaggcgca gcacctcttc ttctcgatcg cctcgctccc gatcttcgcg
    57121 acgggcttcc ccgccgccgt cctcagcctc aactacgtca agcaggtcgc cctctcgaag
    57181 aacctctcga ggcgcttcgc gcggaccggg ctcgcgcggc cgagggaggg ttgacgcgcc
    57241 gccccgccgg gacgggcccc ctggtcggcg ctcacatgag cgccgcgggc ggcctcgtcc
    57301 gggcggtcga gagggcggtc gacgccggct gccggacgct ccaggtcttc acgaagaact
    57361 cgaaccaatg gggcggcaag gccgtcgtcg ccggagaagc cgccgcgttc cggaaggccg
    57421 cggccgacgc cggcctcgcg ccgctcgtct cccattcggc ctacctcatc aacctcgcct
    57481 cccccgaccc gctcgtgagg acccgctccg tcgaggcgct cgtcgacgag atcgagcgct
    57541 gcgacgcgca cggcatcccg tacctcgtcc tccatcccgg ctcgcacggc ggcgacggcg
    57601 aggaggtcgg cctcgaccgg atcgcccgcg gcctcgacga ggcggcggcg aagagcccgg
    57661 cttcgggaac caccgtcctc ctcgagacgg ccgccgggca gggggcctgt ctcggccacg
    57721 acttcgccca cctcgcgggg atcctcgccc gcgccgacag ccgcccgcgc ctcgccctct
    57781 gcctcgacac gtgccacgtc cacgccgccg gctacgacgt cgtctcgaag gaaggctgga
    57841 ggaggacgct cgacgcgctc gacgccgcct gcggcctcgg cctcgtgaag gtgattcatg
    57901 cgaacgactc gaagaaggag cgcggctgcc gtgtcgaccg ccacgagcgg atcggccacg
    57961 gaacgatcgg cgcgaagggc ttcgcgaacc tgatgaccga gcccgccttc gaggaggt
//
LOCUS       DAOONL010000012        57607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_363_length_57607_cov_20.294151, whole genome shotgun sequence.
ACCESSION   DAOONL010000012 DAOONL010000000
VERSION     DAOONL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 57607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57607
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_363_length_57607_cov_20.294151"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLP50_03465"
     CDS             complement(<1..1123)
                     /locus_tag="PLP50_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA50638.1"
                     /translation="MLSFEVRKPGGESEIRKVHRDVIHLGASSGNDLVVRARGVLGRH
                     ARISVAGEELRLDVLGTAPGSDVYLNGAIVKSAALAAGDRIQIGEATITLLNGPAPNP
                     NRLAAPPPRGRDAFSVAAAALAPPPPPPAPPAAQAPRVPEPRPRTYEPVHAARPAESR
                     PSSLSAAQGFRLADVWGELFGRLRASATLEERLQEVASYLSSSTPVHSVAFLSVADSS
                     TGEAIAAIWKGTLPRLGRRSVDEVFASGGPTDIFEAGMRLRVYPVTRPGAEAVGLLVL
                     PMELAADPLLNDLGVALAAAIGFVHAERADAPFGERTSAHAPAAMVTAPLEDPVTAVV
                     GSSLAIQNLRVSLQRIASARAPVLVIGDVGTGKTLVAETL"
     gene            1260..2228
                     /locus_tag="PLP50_03470"
     CDS             1260..2228
                     /locus_tag="PLP50_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPA50639.1"
                     /translation="MPRPLTDAVPFSAPRDGDLEALVATLGAALAPDAFELVLLCPVT
                     GRRFRRRLAGGRDSGLRPAPASRNGGLPNGNVLTVPFGEEEGWRGRLYLVRRRFRPWT
                     GAESMRFSLLAPLACQLLTRLAEAPAARCARERLLALAEATEAPAMIVDRGGTVLYAN
                     GAAEELLSRDSDDGPAVLSGDRRTIPLASRLLQLAARENPTTERMALTDGRCLEARIV
                     DCPGDGTSAAPPARIVLLKERAAPTIDDVRPHLHARGVSDREAEVVSGVLRGLRNSEI
                     AGELFISEYTVKDHLKHAFRKLSVASRGELLRALHAVPGRPPARVA"
     gene            2303..5839
                     /gene="smc"
                     /locus_tag="PLP50_03475"
     CDS             2303..5839
                     /gene="smc"
                     /locus_tag="PLP50_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPA50640.1"
                     /translation="MFRLKKLEIHGFKSFVDRTSLTLPSRLTAIVGPNGSGKSNICDA
                     VMWVLGEQSARALRGTTMEDVIFNGSARRRPLGMAEVSLTLESRGGQWADTGGEVVLT
                     RRVTRDGTSDYILNGRKARLKDIQDLLLGTGLGVRAYAIIEQQKIDLILSAKPQDRRR
                     LIEEAAGISKYKIRKQQAEVKLEETRANLLRLTDIVSEIERACTSLKRQASRAARWKE
                     QADVLSGLKKRLLRLKSERLVAATEAAEAALKSTTDAEGAALAESGRLDAAFEEARHA
                     AEEAERLRREKADAFAAAREALLAAEAAVTAAAREAEETEARRELVGSQRTEAEEDHL
                     SSVEEATKAEQLLAEAAEELAAAEAKAKETGEALAAATRDESDATTRREGSRRELLAA
                     AARATEATNREREAALLVDRLGFALTKVEERKGRAELQLQERRAAMEAARDLEAETNE
                     RLAEARRRLEEAGVSRRSAEERLASLKEERERATRAFHALDKRLGALETLLAAREAGG
                     EAARRALETSGAKADGVLADHFDAEEGFEHALDAALGGALEAPVVRDRAALASLIETV
                     RASHLGTARFVHPIPETPFPLKPADPRVLGLARDLLTARPGDEALSAALPDALVVASV
                     DDALALAELLPERTFVTKDGVVVRGSIVEAAGTEVPGEGLFTVRRDLKNVGAEKDTLS
                     ERLAALEEEISGTGAQAGEAAQAVEREIAASRRAEREHSEALARLSAARDEATRAERE
                     VATILEEEKQLRADLLRVGEERAKALELSAQKTGEMTAMEAAIAGLESGLESARAVRL
                     SAQQADGEARAARDVLRERRRAAQEAFQTHRNRADAAARRLAELSQQITALGERKERA
                     LAAGEEARERREEAVVAASEAEAALKQSDAAAVQRRGGLEELDRGAHAAREALDAARR
                     ARFEAELVVERRRGDREHLSETCRAEFGVPPEELPPVERAEGEPEPDDEALAAMQEEV
                     VQKTVALERLGPVNHAALEEFDVESKRLQEMSTQKLDLEKSLDQILETIRTINLTSSE
                     RFTQAFVAINANFAQVFQKLFGGGTASMQLLDESDPLDSGIEIMAQPPGKRNQTIGLL
                     SGGEKALTAVSLLVSIFRYKPSPFCILDEVDAPLDEANIDRFTTLLNELSEETQFVLI
                     THSKRTMETAQALYGVTQEEPGVSKIVSVRFD"
     gene            5947..6699
                     /locus_tag="PLP50_03480"
     CDS             5947..6699
                     /locus_tag="PLP50_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPA50641.1"
                     /translation="MEISWFVSRDGQPAGPFSEAELVTAALAGEIGPDTLVWHEGMPA
                     WTRLIDEPSAPSLPGISRRMTDCSECRRSVPLDETVPFRGHTFCAACKPVVLMRIEEG
                     LPPCQGPAFARFGTRLSARFVDMLGLWIVTTAIQIPIVGIPLITQITPDSPGFWVRQI
                     AAMLVGVAGNAAYYIVLHGRSGQTLGKRLMRIRVVRADGSPLGFGKATSRFFADMLSS
                     MTLFIGYLMAAFDEEHRALHDRLCDTRVVELR"
     gene            6696..7289
                     /locus_tag="PLP50_03485"
     CDS             6696..7289
                     /locus_tag="PLP50_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50642.1"
                     /translation="MTASLTCGSCRSPIAPERLGMDATLACEACGALHLGEVFPAFHR
                     ERPAGATGEDVEAAGEATCFTHSRKRAVGACGRCGAFVCRLCELSIAGERLCPRCLDT
                     GVAKGTIPRLETGRLLHGRIALSLATLPLLVFYFTLLTAPAAIFVAIRFWKAPRSLVR
                     PSRAGHVVAVVLGLAQLVGWAFLVSMLFRMLTEGRGA"
     gene            7289..7786
                     /locus_tag="PLP50_03490"
     CDS             7289..7786
                     /locus_tag="PLP50_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50643.1"
                     /translation="MVEYRKLPGRGRRFTSPRWLDVMAPRTTVWLGSDHLLVVDDTGY
                     SERYRRFPYADIQAIVVRPTKTYGTAAAVLALLAAVPLAGSFLTGEVGAWILRSLGGV
                     FLLILAVHALRGPTCRTTLQTAVHVEEVPSWKRLRVARRGIARLRPLLQAAQKETVPA
                     TPPPT"
     gene            7783..8106
                     /locus_tag="PLP50_03495"
     CDS             7783..8106
                     /locus_tag="PLP50_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family protein"
                     /protein_id="HPA50644.1"
                     /translation="MSAPLVLPCFRHPEREAAARCPACRRAFCRECVTEHDGRVLCAS
                     CLRAAASPAAPPRRRIAERFAAAAGFLAAWLFFFLLGRLLVALPSSAGEGAGFGDPPT
                     RSEPR"
     gene            8103..9698
                     /locus_tag="PLP50_03500"
     CDS             8103..9698
                     /locus_tag="PLP50_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50645.1"
                     /translation="MRPKASGDDAPRALDLLEDGAFLLASASPRALLLYAGGTIPFLG
                     AALLLGFDLARAGGASASLPAASFGLALLFVVMKTAQALFCERLRVEGLSSAALDTPP
                     GTLAIAARQAALQPWSLVLLPLALLATVPYGLTAAFFQSAVAEGLRGSSLADLAARAR
                     RHAGVWQGQNHVLLLVLLAFRIFAFLNVFVLLVVLPLLLRTLAGVETDLSRAAGHLLS
                     LPLLAVSAAVTHLLVDPLVKAVYVVRSFHVESRRTGEDLLAALRRRGTAMVVFLLAAL
                     PARAAAPSVDPASLDRALDEVLSRRRFAWHLPKEPAAGGSGVFEDVVRGLWEMVSGAA
                     TAVLRLLDRLARAIAERLRWGRAEGSDRAWAFGLAGEQLLLLTALAVVAGALALLIVR
                     IVRKRQLRAPEPAAPTPTPEIDLLADTVAADAKEPEEWLALGDEWIAKGDGRRAVRAL
                     YLAGLGALARGELVRLAPHKSNGDYSRELERHPGASPSLSPLFGSLATEFDRVFYGTH
                     RVSAGSLAAFKEDVRALRALAIP"
     gene            9670..10851
                     /locus_tag="PLP50_03505"
     CDS             9670..10851
                     /locus_tag="PLP50_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPA50646.1"
                     /translation="MHSGPSRYRRSSGILAALLCATVAAGAYFFLDLALARGDVYPPY
                     SSFRSDPLGTRALLEALAEQPGLDVRRLLSPLPAEPDAGAAVVFVIGPTVEAFAEATT
                     KELEEAEAFLRAGGRLVLALRPVGSTDIPGTAPGVGKEPARRPRPAVGLASRWGVAIR
                     RAGSAPVAAARRAPGPDDPHGSLPEALTWRSAARLELGSDRWTTLYAIDDAPVVASRR
                     FGRGTLVLLTDASRLTNASMRKERASVLLAHLVGEKTTVLFAETHLGVEEREGVMVLL
                     RRYRLGGLLLAALLVAGLWAWRNATGLLAPQEAPGAIPGAGGRDSFEGLVRLLTRGIP
                     PSRLLDAALAEWSRTSGTPTGEMRRLADEARRTTEIPAAYAALCRAAHPEARARKEPH
                     A"
     gene            10844..11800
                     /locus_tag="PLP50_03510"
     CDS             10844..11800
                     /locus_tag="PLP50_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPA50647.1"
                     /translation="MPEPVQDVLARARTEIGRVVIGQERAVDFALVAVLARQHALVEG
                     VPGVAKTLLVRTLAQVLGCDFARIQFTPDLMPADVTGSSVFDLERRQFTLVRGPIFTT
                     FLLADEVNRAPAKTQSALLQAMQERQVTIDRETHPLSPSFTVFATQNPVEFEGTYPLP
                     EAQKDRFLLKIPMSVPPRGDELALARRTLGPDAPEEVLARGEVRPVLGTGELESVRPE
                     IAAVVVRDELVGYAVDLARATREEESVLLGAGPRATQGLLLAARALAAISGRDFVTPD
                     DVKALAVPVLEHRLVLRPEFEIEGLAPAEVVERVLQRVPVPR"
     gene            11797..13143
                     /locus_tag="PLP50_03515"
     CDS             11797..13143
                     /locus_tag="PLP50_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPA50648.1"
                     /translation="MIVPSQRLLAFTGATLLPLALAFAASPEAAPALGGLALVGAAVA
                     VADALAGPRRLRALRAALPPVVRLTRAKPGRVEVTVGGAPIGGLRIGLPLPSEIECES
                     PERRVAERTADSSAEDTTVPFEVVPRRTGRWAVAGLRAEVASPLGFWDLRTSLPASTE
                     LRVYPNLLAEKKAAATLFLNRGRAGLHVRRQVGKGREFEKLRDWVAGDAREDVHWKAT
                     ARRARLVTKEYRTETTQEVYVVVDASRLSGRPALDAAGQPGSTTLDRFVAAALLLGAA
                     TEQQGDRFGLVTFADRVEGFVRAGSGRAHFRACRDVLALLAPRPVVPAFDELLPFLAG
                     RLSRRSLVVLLSELDDAAIAETFLAGARVLARRHLVLAVSLLPATARPLFGGEDRAAT
                     VDDVYARLAGHLQWRTLRQLERSLARLGVTFALLPHESVAADLIARYLAVKGRQAL"
     gene            13140..14147
                     /locus_tag="PLP50_03520"
     CDS             13140..14147
                     /locus_tag="PLP50_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPA50649.1"
                     /translation="MILDLPRFVASARPRWTELERLLSALEAEPDAERDLAFLTRLHF
                     LYERAAADLGKVRTFASEPELVRHLEALVARAYAEVHETRDRRSRLSLRSLAGIVLRD
                     APRAVRRHPGALLLATLLTVAGMAFGALALGVDPATKRVLVPFEHLSGDPAERVRLEE
                     GGETRHADGHRAAFSSTLVTHNVRVAVLAFALGGTAGAGTAVLLFHNGVVLGAVALDY
                     VRAGQTRFLLGWLLPHGTVEIPAFLLAGQAGLILGASLFRRSSRSPLSARLRSVLPDL
                     VAILALVVALLAWAAIVESYLSQHHEPTLPYGLKAAFGLLEGSLLAAFLLRAGREREE
                     P"
     gene            14144..14905
                     /locus_tag="PLP50_03525"
     CDS             14144..14905
                     /locus_tag="PLP50_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPA50650.1"
                     /translation="MSGRSLSIVTPEGITFSLPLAGPLTRFLAWLVDLAAIAALTVLS
                     RQAVLLLGAAGEDVLRALTILLGFAVTIGYGMATEWWLRGQTLGKRLLGLRVVDASGL
                     RLNPSQVVVRNLLRALDALPVFYAVGGAACFLSRRSQRIGDLLAGTVVIRTRHPALPD
                     LEGLFPAEANALAAHALVSSRLKQRISPGEAAIGLSALARRDQLDADARVALFAALAE
                     RYRSLVRLPEEAVDGLSDEQLVRQVVDVLYRGAKP"
     gene            15077..15514
                     /locus_tag="PLP50_03530"
     CDS             15077..15514
                     /locus_tag="PLP50_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPA50651.1"
                     /translation="MRRSLLVRAALLAALLSFPAFASDDVDVRKGGSLWARIEKSGSV
                     RIDGVIVGEIEDDGTIRVEGRIAGRIEEDGEIREDGTVRTRVEKDGTLRRNGTVIGRI
                     EEGGAIRRHGTIWGEAVPCCSSFDDLRRLTALLLFFDRSFFVR"
     gene            complement(15543..16685)
                     /locus_tag="PLP50_03535"
     CDS             complement(15543..16685)
                     /locus_tag="PLP50_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA50652.1"
                     /translation="MTTIVRRLLLSLAALLVAFAPGPARAQEEPSLRIVRPGPDDIPL
                     GESTVEVRLTGHRAGDVVEVFVDGRPAGTIHGEPWTLTWSAGSAPRPHRIEAVLRRDG
                     GEAAVSRVRTRGLGFSATADARVVSLSPIVTDSGGAYVRGLAREDFSVFVDGQPQEIE
                     TFESVNSALSVVLVLDQSSSMALKLRSARAAALEFLAALKPDDRAAVLTFASNVVGFT
                     PFSTDRTAARKALEAARVSGETALYDAAAVALRKLRESSGRRAVVLFTDGEDNRSRMS
                     VDQVVDLARATEASVFTVAQGSAEVRALLRGLDRLARETGGRAWFISSIKSLPDVFRE
                     VVTELENQYFLTFTPADQRPRTWHRVEVKTRKPGLTVRARKSFRID"
     gene            complement(16682..19990)
                     /locus_tag="PLP50_03540"
     CDS             complement(16682..19990)
                     /locus_tag="PLP50_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50653.1"
                     /translation="MTPAAVPPRVGGPGPGRRRRGSPSRRPVGRLVPLLATVLLPASG
                     LLAEPVVVPGGTASIRRLLDLGDRRTESDFFVEVGRVLLSGTNPADPWNKSEKRKAVA
                     SFAEDLAEWTEKHGCPAVLSTKPETWAATRRALGWLGYRVRGEGPGFEAEPLADPEAV
                     RRQAFLDVLGLPVMDVLERLAAGEDVRVECASGTAELPFSLSAWRETLGLDEKRLNAR
                     NAFLRFVTDVPASRMMMALHGVDARTREELRAVGGPAGGYAGWKLMYEKALDGFALYP
                     EALEIRNGEIRLPGGAAAEPVWEAVVGEPPSDLARFVVKVFGSASGKAAYVIDALRPL
                     SEETARAFVLGASGGGEEAVERFERLYSAIGKTGRSYGNSQRDPWDFTQLAGYLRSAA
                     GGEIELPGGAGTWLEALESPSMPEGEAELQQLLAAAAERTEGPAEALERILREETSGV
                     SGPLPAVKRFIVVSSLVRDRPDLGDPATLLLLARGVDRFLACYAPLEDLPLGSAETAR
                     RYLFTLNRLDSNGTDRDAELRAGLFLASVELLSALFRSGSVSDDVARGLFASLLGQPL
                     FATPRTAQAAGVGSFDLWLHGELLGALRESETRFLGGERAGSLSWEDEEDRGPAKSAD
                     DLLFAALSGFRPPATLAFRGGSYVYDAMEDAVARRRAFARTQDHVPLWVLARAAAERE
                     SASASARAGDAEATRAAVSALLESLGAIPPAREADERVAATASGARHVLGTLQAATRE
                     TVLALLEAGLPRLDALRAERTLEALAVHVYAGHVLDSSDLLFADSLLVKRHSLSWGPR
                     KGIVLPSPFQPARIDTPGEGAPLRLAGGWAGIGAPLGLLHAEGLVYDAGGFLSNDAVR
                     SGLVLPSVLFTPARLDDEGLRFVALACRSAEQLPVALAGLPETERHDAWSRLARDLLP
                     ASRRNRLAREGAAVAADDLSPSDLLRIGRRLALRADGALPDVPAAREAREVWARRVER
                     LGEARAGALLAELGPRAFHWEGRSRLGDVTLPSYERLSEYRVPQVFGDRLHDLPIAVA
                     RSVSDAGDPAALVPLVLRSALEDLMRRARMAYAFDWRPLAATSGSFWPAQRDAVVGAA
                     LSEGRVRRMEVPGP"
     gene            complement(20004..21101)
                     /locus_tag="PLP50_03545"
     CDS             complement(20004..21101)
                     /locus_tag="PLP50_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50654.1"
                     /translation="MADSGRGIEQQLEESVRRVLGALERMPVGKIEVVANEVSRAVGQ
                     VVSQVDRASRSPTREERRLEKRLREEDEASLPQAWVMAGAAAVAGLIGLTQPQLFWFF
                     FVGFGFAMGAADIFAKVRRRERRQAEAAEERGAAVSGATAERAVPAPLVTPPPPERAE
                     SPALAAVSAREARVDGICNRLLSELEGAPPLVRELLRRPEQTVEALRAASRELSRRER
                     ELRGMLSEDEGERLVGERAELAARAEAAPDAVVKSRLAAALEALDAQLVHRAELTTAA
                     ARIEAEGTRILYSLESLRAQVLRAFAADAAAADVTRESLKGGLETLRGEIDAVATALE
                     EVHGVGAPPVAATAGGPSAAARAALRQAGTR"
     gene            21366..23060
                     /locus_tag="PLP50_03550"
     CDS             21366..23060
                     /locus_tag="PLP50_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPA50655.1"
                     /translation="MILNPKRLGELPLDARSREIVEKTIAFFEAKGKKRLKQDDFDRV
                     WYSDFLRFVKEERIFADLLTPPELGGPDARWDTFRNCYVNEVLGFYGLCYWYTWQVSI
                     LGLGPLWMSPNETARRRAADALANGGIFAFGLSEKEHGADIYSTSTTLAPRGDAFVAN
                     GTKYYIGNGNEAATVSVFGKMAGSDEYVFFAADPKRPGYRLVRNVVASQSYVAELALE
                     EYPVAADELLLRGPEAWDASLNTVNVGKYNLGWASIGICEHAFHEAIGHAAGRKLYGM
                     SVTDFPHVARMFVDAWARLVAMKVFAFRASDYLRAATLEDRRYLLYNPLVKMKVTTEG
                     EKVIDLLWDVIAAKGFEKDMVFEMAARDIRALPKLEGTVHVNIALIVKFLASWLFRPA
                     SLPEVGRRAEPAHDAFLFAQGPARGLGKIRFHDWAPAFERYARIPNVALFREQLGAFR
                     ELVASAAPTEEQSKDVDLLLAVGDLFALAVYAHLVLEGAEHHSISDDVLDQAFDVFVR
                     DFSSYALKLHERSGATERQAAMALRLVRRPAFDAGRFERIWKNEVLSLRDTYRMSE"
     gene            complement(23068..24324)
                     /locus_tag="PLP50_03555"
     CDS             complement(23068..24324)
                     /locus_tag="PLP50_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA50656.1"
                     /translation="MRPSRHPGARLHARIYVAVLASVVLVALLAGAAWHLHYGREPVE
                     NGLGLTAEIAGELLPPPDAPVALQQRALERWGSRTRARLSLFTPARERVAAFGEPLPP
                     PPAGFAGSATWHPAGVGPAVVLRLPDGRWLVAGRAASGRPALGFLGFLLLAALAVALA
                     AWPIARRITRRLERLRESVEALGGGDLGSRVPEEGKDEVALLAASFNRAAARIEALVG
                     AQRRLLANASHELRTPLARLRVAADLLDGEPAAKGEVARSVVELDALVEEILVASRLE
                     AGATGEPFEEVDLTALAAEECARAGAALSGDVVLVRGDPRLLRRMLRNLLENARRHGG
                     GGPAEVEVRRAASGAEVDVLDRGPGVPPDLAERIFEPFFRLPGAPDGGAGLGLSIARQ
                     VARRHGGDVSCAPREGGGTRFRVRLA"
     gene            complement(24321..25019)
                     /locus_tag="PLP50_03560"
     CDS             complement(24321..25019)
                     /locus_tag="PLP50_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50657.1"
                     /translation="MATRILMIEDDARLAEMVARDLGRSGLEVTAAGTATEGFGRLAR
                     EPFDLVLLDLMLPDADGLDVCRRLRASGSAVPVIMLTARGDPTDRVVGLELGADDYLG
                     KPFDPRELLARIRAVLRRPAAVAGAAPRVLRFGRLEVDSDARVVRVDGEERPMTAHQF
                     SLLLAMAERAGRVLSRERLMELVRGEALEAFDRSIDVHVARIRAAIEDDPRSPRRILT
                     VRGAGYVFARSQDA"
     gene            complement(25111..25650)
                     /locus_tag="PLP50_03565"
     CDS             complement(25111..25650)
                     /locus_tag="PLP50_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPA50658.1"
                     /translation="MNEITPIVPEASVNAPAKRRRRGRTLFLGALFLGAAAVLTTGAL
                     QAFGLGPFRSGACSHMDPAQVEKKIDRIAGWIVEDLDGTPEQQAKLAAIAKAAAKDLA
                     PLHDELVAGKQEVIAILTAEKVDRAALEAHRVRHVQLMEEASERLVAAVADAADVLTP
                     AQRAEAAAKLETFHSRFGH"
     gene            complement(25840..27399)
                     /gene="murJ"
                     /locus_tag="PLP50_03570"
     CDS             complement(25840..27399)
                     /gene="murJ"
                     /locus_tag="PLP50_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPA50659.1"
                     /translation="MSAARRSSSANALLVGAGILLSRLAGFVRERVFAHYLGSSMAAD
                     ALRAALRAPNVLQNLFGEGSLSASLIPVYSRLLGEGREEEARRTARTAGTLLALLAVL
                     LSALGVLAAPLLVDLFTPGFSGERRELTVRLVRILFPGTGLLVLSAFCLGILNSHRRF
                     FLPYAAPVLWNGAIIVALVLAGTKAGAEIAVFAAWGAVAGSLLQLLVQLPQVARLVGT
                     LRPALDTGTPHVRTIFRNLLPSVGSRGVTQVSSWVDQVIASFLPGGAVAMLGYAQTLY
                     ILPVSLFGMSVSSAELPELSSEAGAETLRERLDAALRRVAFLVVPSAVAFALLGDRVV
                     ALVFQTGAFGAEDVRIVWIVLAAASVGLLASTLGRLYASAFWALHDTRTPLRFAMLRV
                     TASAALGAFLAFALPRLLGLDPRLGLAGLTLGSGLAGWLEMLLLRRALNARVGKTGLP
                     ASFSLRVWGAALGAAAAGFGVKLAFASLGPVLLGALVCGAFAAAYALLALALRVPEAT
                     ALAASLRRRLG"
     gene            complement(27396..28499)
                     /gene="mraY"
                     /locus_tag="PLP50_03575"
     CDS             complement(27396..28499)
                     /gene="mraY"
                     /locus_tag="PLP50_03575"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPA50660.1"
                     /translation="MIFWIGKLLAPFPELSFLRLAEYLSARSIGAALTAVLLTLWVMP
                     RVIWWLQRKAFVDQLRETGIPSAFDKAGTPVMGGVVLVGVVAFSSLLWCNLTSGYALL
                     VLLGMVWFGAIGFVDDRAKMRARSGDKGMSEARKLMLQGAFAAILVAFLASPWNPLPD
                     AEAARLHVPFLKAAVLDNPWVYFPFVFLFVLLVGNSVNITDGMDGLAIVPSVFAIAVL
                     GVFGYLLGNAIWSRYLVYPFLPGAGELAVFACAFAGAGVGFLWFNAYPAQIFMGDTGS
                     LSIGGSLAVASVLLKQEFLFVILGGLFVAEAFTSQVQDKIGIKLLGRRLFARAPLHHA
                     MAYNGLAETKVVVRLWIVSGILALAALSTLKLR"
     gene            complement(28496..30514)
                     /locus_tag="PLP50_03580"
     CDS             complement(28496..30514)
                     /locus_tag="PLP50_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPA50661.1"
                     /translation="MKRGTRILLTGGGTAGHVNPALAIGAALAEEGSETLFVGVRGRA
                     EEQVVPKEGIPIRFVRASGFPGTRPSPALVRFLLDLGVGTLQAAGVLLRFRPDVLVGT
                     GGFASAPVIFAATLLRKLRLLRSRVFVHEQNAVPGKLNRLVGRFAEKVFVTFPETLSL
                     FPGNGAVTGYPLRKRIGHVSKEEARARLDFAIPEGRTVVFVFGGSQGSRSLNRGLVDA
                     LGDLLPHAERLFVVHGTGLFKGGGYDAGADTEARLAARYSKEERERIATFYVSRSYFH
                     DIASVYAVSDLVVIRGGSGSLNEVARMGLPALVVPKANLPGDHQVANARALERAGGAE
                     VLYEETVLRDGHLAEELDGALLARRVLDLIDDPDRLAEMSRAGRDVLGADAAAEIAHH
                     VRHGRGTGTIEPTLVPAPHVVSNAALLSRLEKAAAAEGRRYRAEAHVEPRDLPWFRSR
                     AASLLANEGWEARNLGVKLLGLLGATEKVPLLLALLGDRTPAPLLQRLLGGDFAQVGF
                     VRRNAFTALGRLGVVDAGVEAALLAGFADPYWEVRAEAARTAARFAGRLERRDDVVAA
                     LVGGLSDRNLEAAAAAAEALGAIGGEADALPALLRLSDYKFWKVRAAALRGVLALVER
                     GEARDRARLATALPAFLLTSTDFRPQFEMKLSYRRVLEALSRGKESPR"
     gene            30646..31617
                     /gene="corA"
                     /locus_tag="PLP50_03585"
     CDS             30646..31617
                     /gene="corA"
                     /locus_tag="PLP50_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPA50662.1"
                     /translation="MLSIHLLEDGKGLQLLEDTPLASAWPGLAGRSIWVDVEDPTDAE
                     ARALADVCGFHHLTVEDALTPVQPPKVEEFEGYLFLLFRGLRPTDEGFELESYKLAVY
                     LGPSFLVTVHRRPVAACREVSRLVLERDLPMESQMDRVLHALVDRMMDEILPILDRFE
                     ERLEELEEAVFSRSPHREVLQELLGMKRQLLTIRRLVAPQREMLGRLARRDLPFIEER
                     TSHYFRDVYDHVARMEETTMVLTDLASGALEAQLSVASNRLNEIVKVLTIFSAIWMPL
                     TFLAGVYGMNFEFLPELKIRWFYPALWGAWIAIAAGMLAVFRWRRWI"
     gene            31750..33771
                     /locus_tag="PLP50_03590"
     CDS             31750..33771
                     /locus_tag="PLP50_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA50663.1"
                     /translation="MSGLCAIGRHGGPRPSVLRCAALALALLGAPPATAQEKSLDDLL
                     SLSLEDLAAIEVVSAAKVPATAARVPATVRVITAETIAENGYLTLEDALADLPGFQLR
                     NILGFNAYAFLRGVPSQNNAILLLVDGIQVNELNSGGFYGGGQLNLANVERIEVVYGP
                     ASALYGTNALSGIVNVIRKDPAKGTGGKLNASLGSLDTSLLDARYAWTSPGGDVSVSV
                     AGMRKRTDKADLRGARGDGNWTDQADNYEDDLSFDGLVRWKGLSVGFLVQDKEASRAS
                     VQRTADTTFLDHGVGWHIRFLNAWARYSLKERGPWSAHSTLYVRSSTVMDDTVPVIER
                     ATASSPGSQQRWYRPGELVGAETQATWAPTKRFSISAGLVLERERLSESYSITTSGSE
                     LEPAPPPPQPERITNDLASVYVEAQAGLTGELRLFAGLRHDDATSYGNVDTPRAGLVL
                     NRGRLTAKLLYMEAFRAPKPWDLTDGLGNPDLRPEEMRMVEASAAFSFSPHLQADVSV
                     YSSRLDALLTRSGEGDEWRWVNAGELETRGVEVSAELRKGRFVAWANATFCESEDETG
                     HQVAEIARSGANAGVRYLVARGATLGLRGSWLGSRRNPKVVPATEDDRIEDALVLHAS
                     ASVALGHGLEARLFVDNLGGTTYDHPSNLPPSRYRQPGRTFRVQAGWTF"
     gene            33759..34370
                     /locus_tag="PLP50_03595"
     CDS             33759..34370
                     /locus_tag="PLP50_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HPA50664.1"
                     /translation="MDVLTRATVPRPARLPARRLVRALTRALRNAFVAALLVPAASAA
                     AQDRGLEARVKAAYVYNFLTFVERERAEPVPAGAPVRICVLGDEAILAELKGLTGREV
                     RGRKLEVVSGPEAGPPPSCHLLVIGRSEAGRLPEILSEVSGAGVLTVSDIPRFARGGG
                     MIGFRTEGGRVRIEVNPAAAAAAGLKVSAKLLEVARVVPRERP"
     gene            34367..36259
                     /locus_tag="PLP50_03600"
     CDS             34367..36259
                     /locus_tag="PLP50_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50665.1"
                     /translation="MSFRRVGSIRAKLMLIGMATSLCSLLLAAAAFGLVDRYRDRKGL
                     VEDVSTIARLVADRSTAALAFDDPGLAAENLAALRIKPSVVAACTYAGDGSVFAEYRS
                     GREEYRFPRAEDGPSHRFEEGHLSLFEPVVLDGTRIGTVHVRASLRELAVLRREYLLS
                     ATAIVAFGALVAFALSTRLQRLVSAPLARLTETARHIATEKDYSVRAPAEGKDEIASL
                     ARTFNEMVGVVEAESAASARAQAALRASEARMRQIVDLVPHMIFVKDHEGRFLLANQT
                     VAEAYGKTVPALVGRPQAELGLDAEQVRGWREEDRKVIEEGVVCFVDEAPFRDASGTL
                     RFLQTTKVPLLLEDGVTRAVLGVGIDITERKKADDELRRYHDHLEDLVKERTSELAVA
                     KEKAEESDRLKSAFLATMSHELRTPLNSIIGFSGVLLQGLAGPLNEEQQKQMGMVCRS
                     AEHLLALINDVLDISKIESGQLTFSIETFDLRASLASLVRSAAPLAEKRGLSLEHEIS
                     PSVGEVTTDRRRVEQIVLNLLSNAIKFTERGSVRLVVTAGDGRATIRVVDTGIGIRAE
                     DLDKLFRPFRQVDAGTSRHYEGTGLGLSISRKLAELLGGSIDVASEPGRGSTFTVVLP
                     LRRETP"
     gene            36256..36648
                     /locus_tag="PLP50_03605"
     CDS             36256..36648
                     /locus_tag="PLP50_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50666.1"
                     /translation="MKAKILYIEDNTQNFYLVSFILTAAGCEVVWAKDGPEGIEQAAR
                     GWADLILLDIQLPGMDGYAVARALRERPALSLIPIVALTSYAMAGDREKALAAGCTSY
                     IEKPIDPAKFAAQVEQHLLDARAKGEGS"
     gene            36645..37874
                     /locus_tag="PLP50_03610"
     CDS             36645..37874
                     /locus_tag="PLP50_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50667.1"
                     /translation="MSRVLIVDDIVENRYLLETILKAGGYDVVSAVNGAEALAAAEAA
                     PPDLLVTDILMPVMDGFELCRRWKADETLRGIPLVFYTATYTDAKDEVLARTLGADLF
                     LVKPMPPDALLEAIGKTLASPRTESPAADAGKALQGYSEALFRKLQRKVGQLEAEVAT
                     RKRAEEEVRALNAGLEERVRSRTAELETSIREMEAFSYTVSHDLRAPLRAIDGYAALL
                     DSEAAERLAPESRELLRRIRASARTMSTLIDELLEFSRTGRTELRRTRLDMGEIFRSA
                     FEQLTTPAERERVELRVDALPPADGDPALVRHVVTNLLSNALKFSSGRARPVVEIGSR
                     EEGSRVVYFVKDNGAGFDMRYVDKLFGVFQRLHSPAEFPGVGVGLAIVQRIVARHGGT
                     VSASGRIGEGAEFTFSL"
     gene            complement(37911..39254)
                     /locus_tag="PLP50_03615"
     CDS             complement(37911..39254)
                     /locus_tag="PLP50_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA50668.1"
                     /translation="MNELAGLPPLRPEAPVPTPRVVAALLPNGLAVQVVPWRGVPLAS
                     ARLVVRGGLSDDPRALPGLARLLAAGLREGTASRSGAELADLAQEAGGDLSAGAGPDA
                     LTIGAYGLAARLPLLLDLVADVVRRSAFPADGVARARHLAHEELETSESEPSFLAVRA
                     FAREVYGAHPYATISPTAASIEAVTPELLAAEARRRLRPERALLLVAGDVEPDATIAS
                     AGVAFGGWVPEGTAPASGASPTATPGPRRILLLDRPGSVQTNLLVGNLGLPRTDPDAY
                     ALELAMTIYGGSFTSRLVQNLRETKGYTYSPGAGASWLTGCGTVRSFAAVRTEVTGAA
                     LGEVLYEMQRMATTDPTDEEMERAFRRDAGSHAISLQTAAGIADELARLWVLGLSPDE
                     IGRYVPGLEAVTKGDVRRVSRRVFGTGSARIVAVGDAAAIREELGTFGEVVGSGD"
     gene            complement(39251..40600)
                     /locus_tag="PLP50_03620"
     CDS             complement(39251..40600)
                     /locus_tag="PLP50_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA50669.1"
                     /translation="MHDRRRDLGDPADGHRPAAPEGLTLQLPIETLVLPNGLTVVVSE
                     NRSAPVFGLCVLYRIGSRLEPRGRSGFAHLFEHLMFEGTPRAGKGVFDRVCEGSGGSN
                     NGQTRPDVTLYVVDAPASALERILFLEADRMSGLSFGQESLDNQRDVVKEEILVNVRD
                     DPYGLFEYGELPLALFEKWENAHDGYGDFHDLDAATLADVESFFAAFYRPDNAVLAVA
                     GDVGAADVFALAERHFGPIPRGAACGRPDLSEPPRAEPVLLVREAPLARTPALAMGWR
                     MPERVRPDAAPLMLLGELLHNGRASRLYRGLVEGREIATELSGGFNPFQGGVWYEGTT
                     LFLSRVGFRREVPAESVLAAFDEEVARIARDGVEAAELARVKTKVLADTWAGLEPRLG
                     LAIELAQATAIAGNPEALLDLPRLVEAVTSDDLRRAAEWLAPERRAAIAKVPPKGER"
     gene            complement(40531..41727)
                     /locus_tag="PLP50_03625"
     CDS             complement(40531..41727)
                     /locus_tag="PLP50_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPA50670.1"
                     /translation="MTTFLDSPAAVGAAASVPFGFQLSDDQLAVRKMVREFAESELRP
                     RVMEWDEAQVFPREAIARLGELGMLGVIIPEEYGGAGLSYIDYVGVIEELSRVDGSVG
                     ISIAAHNSLCTNHLYMFGTEEQKRRWVVPLAQGKLIGAWGLTEAEAGSDAKGTRTTAV
                     LDGSEWVLNGSKNFITHGSVGDVAVLMAVTDKAAGTHGISAFVVDLHQKGIRAGKKEN
                     KLGLRASDTATLVMEDCRIPNENLLGPLGTGFSQAMQVLDGGRISIAALALGMAQGAF
                     DCALDYSRQRVQFGKPISEFQSIQNYLADMATEIDAARLLTWRAGWMKDRGMKVTKES
                     AMCKLYAGEVSVRVANLAVQIHGGYGFTKDFRAEKFYRDVKLCTIGEGTSEIQRMVIA
                     RQLLKA"
     gene            complement(41793..44042)
                     /locus_tag="PLP50_03630"
     CDS             complement(41793..44042)
                     /locus_tag="PLP50_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPA50671.1"
                     /translation="MTRTSSLAAALAAALLAASCTPRPVPVTVVPSPPPTPAPTTAPT
                     PPPSVRTAFPDVPEPRLDVGVAVDRAAFRLPAGEWLLRSGGEVRRVVGALEARPAASE
                     ADAAPRFQVQAGSFTARVTAEAEAVRLAALLGLPAAVAEGGGRFAVRLGPPGDRASLD
                     PLLYRVRRDAVPEAFLVGLADPGRPDRTLLVDVPGGRLEVPSPVELAAADATLLPVNG
                     APYRGTLLLVATPRGTLHVVNRVGLEEYLLGVVPLEMGPRVYDELEALKAQAVAARTY
                     AVKRRGDFAAEGYDLCATARCQVYGGASAEQPLSSEAVRATAGQVLLWNGVPADTLFT
                     STCGGRTEDAVVVFPSYAPAAFPYLRSVACRGEERIAFSSTSPVAGHDFGALAVRGAA
                     LLHAAGRRGASWTDLKVARGLLTDRLGLPAGAPPATLAPEAVYANLSGVLGDPRLLTE
                     VAERDAAPKRWGEPARTVHTALSRFQLAGPTPLPTHRSFTPHEAAGLWASLLLRAGGL
                     EELEGRLVGAPRGKVVLKTSKGREERTLPPDALLFRGGGEAWTPASSLDVAPGDRVRL
                     LSRDGKVLAVLAQVPAVDGLFERESAWIHWVRRFTGAELMRKLLERDASRMGSVVRRV
                     DVLARGVSGRAAKVRVTTDRESFDLSGLEIRFALALPESLFTHVTGKDGSGAPVHTFY
                     GRGWGHGVGLCQNGTFGMALAGRTHAEILAAYYPGTALGAWPPPPEPERPGSPEARPA
                     TGGEPPPAR"
     gene            complement(44047..44766)
                     /locus_tag="PLP50_03635"
     CDS             complement(44047..44766)
                     /locus_tag="PLP50_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50672.1"
                     /translation="MDEPAVSVPCPVCRGFGRLERADGSTVPCECRAEEIARARRAGA
                     RIPERYRNCRLQNFKDLRNESLKRAKAVAKRWVEEWPAVDAGLLITGPVGVGKTHLAV
                     AILNELVDTKGASALFCDFSDLLDRIQASFGKGGDESQDDIVAPYRDTDLLVLDELGA
                     RRPSDWVREILYGLLNTRYNRRRLTILTTNFADEPDARGGETLEVRVGAPVRSRLWEM
                     TQLVPISADDFRRAHGARAFA"
     gene            complement(44799..45686)
                     /locus_tag="PLP50_03640"
     CDS             complement(44799..45686)
                     /locus_tag="PLP50_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPA50673.1"
                     /translation="MNQRRADEDSGLLRRYLQEISKYPPLSAEREKELARVIQDRLVP
                     EERRKEAIDELVRANLRFVVYHAKGFHSPNVPFIDLINEGNIGLIQAARRFDANRNVK
                     FITYAVWWVRQAISHALAEQAGTIRIPHKQATHQFRAVRARDELQRELGREPTHEEIG
                     ERTGLDPATVEMLLAVTRNTESLSQGVGSDEQDRTLEDVLEQTMVSSADDDMLRRASA
                     EQAREFLGELKPKERAVLCRRFGIPEDGSEGEREPMTLQEIGLELDLSRERVRQIEAQ
                     ALQRLRRGVKARRLLSVLR"
     gene            complement(45739..46161)
                     /locus_tag="PLP50_03645"
     CDS             complement(45739..46161)
                     /locus_tag="PLP50_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPA50674.1"
                     /translation="MAASREGSGRGRTRRDDYVLIGVVAERFRVHPQTLRLYEREGLI
                     CPPRSSGNTRLYDRETVERLEMILTLTRDLGVNLAGVEVVLDLRARLSKMEGEVERLM
                     EAFRSVAAEHASRAAERPHPPQNALALRPRAPLARRTS"
     gene            complement(46166..48070)
                     /gene="dnaK"
                     /locus_tag="PLP50_03650"
     CDS             complement(46166..48070)
                     /gene="dnaK"
                     /locus_tag="PLP50_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPA50675.1"
                     /translation="MSKIIGIDLGTTNSVVAVLEGGSAEVIANSEGGRTTPSVVAVTK
                     SGERLVGQVAKRQAVTNPENTVYSIKRFMGRRFDEVNEEMKMVPYEVVRSANGDARVT
                     VGGKEMSPPEISAQILTKLKEAAEAHLGEKVDRAVITVPAYFNDAQRQATKDAGQIAG
                     LKVERLVNEPTAAALAYGLDKKHNETIAVYDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDNIDQRIVDWLLAEFRKDQGIDLGKDPMAVQRLKEAAEKAKIELSSTIESEINL
                     PFITADASGPKHMSVKLTRAKLEQMVDDLLQRSIGPCRQALKDAGLEAKQIDEVVLVG
                     GQTRMPKIQQVVKDFFGKEPHKGVNPDEVVAVGAALQGGVLAGDVKDLLLLDVTPLSL
                     GVETLGGVMTRLIERNTTIPVKKSEVFSTAADGQTSVEIKVLQGEREMAADNKLLGVF
                     SLVGIPPAPRGMPQIEVTFDIDANGILHVTAKDRGTGKEQKITITSSSGLAKDEVEKA
                     VKEAQAHGSEDKKRREAVEAKNQLDSLVYATEKLLAENADKVPAEDKTAIEAAVADAK
                     KVLETQADDAEALRKSAQEVQKASYKVAEALYKSAPAPEPGAPAGSSTAGSGKSADDV
                     IDAEVVDEKK"
     gene            complement(48183..49478)
                     /locus_tag="PLP50_03655"
     CDS             complement(48183..49478)
                     /locus_tag="PLP50_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA50676.1"
                     /translation="MALEGTLHDFALADILQLIALQKKTGLLTLRGPADTVILGFDGG
                     LLVSAESQARRIDTRLGTLLVKTRSLSPESLTKALEIQGQTLQRLGFILLKNGFCDAD
                     QLRAGLDAQVRKIAYGLFRWTDGDYVFDPQEKIDYDREYVHPIQVEGLLMEGARMIDE
                     WPIIQKVVRSPELVFQRVPVGQRVEAAEDLDGVEAIGEPVGSRARERRDGPICVSRAE
                     WAVYELVDGRRTVGDIVDRTFLSEFEGSKAFFDLLSRGLVEEARRTGVVDERGMSSVE
                     VPIVRPSRLLPAVLAAVLVVLLGAAFLIQHRNALNAFTIPGRRVAAVEGFQKAISLSR
                     LRRLSEAIDAYYLTQGVFPESPSAIVDAGLLGPGEILDPWGRAYRYILQGETGKYYLA
                     GFGADGKTDTDLFFAHAASGGMSGTESHSGDNKEIILIQ"
     gene            complement(49483..51387)
                     /locus_tag="PLP50_03660"
     CDS             complement(49483..51387)
                     /locus_tag="PLP50_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50677.1"
                     /translation="MRRQTGLGPRRPRALRAALALLLLPLLSGLLASAQVLQAPGEAT
                     ESTFRREVRGQWFAWLAAHEEGDPLLAAQKVEEIVKHARKIGLKRLTDLSLAATLLAR
                     REATQGSPEKARWALDAAVRLDPELPEARWARVRLALKAEHLAVPRELVGALRASVVD
                     LEGRRVLLVRASLLLVLTLAAVGLSFAVLLVAGAVRRLFHDLTELAGRWVPPSAEPVA
                     ALAALLLPLLFALDVGWLLLWLFALSLGYADRSVRWAAVAGLVPLLLVPPALDRAAVS
                     LSVSASPVLRGAEALQEKRYDQRVLDDLEAVKTLFPEDADVRFLLGCLYQQLGQNDRA
                     VAEYTVAAQVSTAEVRALVNRGDIRFVDGDFGAAQEDFLEALRRDPRDVRARYNLSLV
                     YGETFRTVEAQEKLTEARALDNALVTKFLDSPTLVKVVSLGYTPDEARAKIGLLQRDP
                     RSRRVLGHFHVGSVVGTWAVPLAVAIPFAMAAAFLLDSLRRKGRGYAVACQKCGRTFC
                     RLCKPPGESPLLCSQCVHVYLKKDGVSIETKLQKVEEVKRRQGTEERIRRVLNLFLPG
                     GEAFLDGRVAKAAAILALFFFALLAALFRDHLALSPRPGAERLAAGTLAWGLVAAGAW
                     VAGQLAARKG"
     gene            complement(51402..51833)
                     /locus_tag="PLP50_03665"
     CDS             complement(51402..51833)
                     /locus_tag="PLP50_03665"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPA50678.1"
                     /translation="MDRRMFTADGDLRDDVKEELAATPPAPETPSAAPEAAAPPAPEE
                     ATPTSPAFLRLLDMLAQTASLYLQGVPDPATGRRAVDLNASREIIDTLLALREKTRSR
                     LSFEETDALEGLLGELQLVYTRLATQARGGGAMPPPPRPKG"
     gene            51930..52760
                     /gene="mazG"
                     /locus_tag="PLP50_03670"
     CDS             51930..52760
                     /gene="mazG"
                     /locus_tag="PLP50_03670"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPA50679.1"
                     /translation="MPPEPRVPRPEAAGEAAAALVRLMSRLRAACPWDRKQTWADLSG
                     YLLEECHEALEALARGSLRDLEGELGDLLFQIVFLAELGRESDAFDLASVAERIHAKM
                     VSRHPHVFGESEVSDAGAVKVQWEKLKEKERAGQAGEAEPPSPFEGVPRALPALLKAA
                     RVTSRAADLGFDWQRDADVLAKLDEEVAEFKAELLAEGATRDSVANEIGDILFTVVNV
                     ARRHGVDPEAALQATNAKFVRRFEEVSRRVRASGRDVRDTPLEELDRTWDEVKRGEAP
                     "
     gene            complement(52773..53252)
                     /locus_tag="PLP50_03675"
     CDS             complement(52773..53252)
                     /locus_tag="PLP50_03675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA50680.1"
                     /translation="MGGDTGIRDVVTGLYVREYFDDVIARELERSRRHGIRLSVLSVV
                     LANFAELTKAGEETALGAVVEAARALQGSVRETDHLFRWEEDEFLLLLFNADAEACAL
                     KVGQLDLLFRPWRTVASPVPHPVRVRVGGATHEGDLVFATVLQAARESARAGAHGPG"
     gene            complement(53285..54505)
                     /locus_tag="PLP50_03680"
     CDS             complement(53285..54505)
                     /locus_tag="PLP50_03680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPA50681.1"
                     /translation="MSPSLLLAPPANAGPAEPFAGASRLELPAELLVPGASAVAIATL
                     GLVALLFWARDRSRTDLLLYTFLASGLASYLLLGLGEGSAGAARAAAALGLLLPALAW
                     LLLVRFLDLPLRKGRISLLALPALALPVVVALPAARALSAPAAAAALVLFGGAALLDL
                     VRLRRPDAALLFAATALLLLSALFDVAVANGVLLPGERLPPLLGPAFLLFTALLLVAV
                     ADQGRRLLEKATTDALTGLPNRATFLERARRELERAQRNGSSLALAMLDVDHFKRFND
                     RYGHPAGDRVLKDAGRAVAGAVRGIDLAGRYGGEELVLLLVDVDAEAAVAAVERVRAA
                     IAEVRLPHVPDAVTASAGVALHHGAFERCGVADLIRRADAALYRSKAEGRDRTTLEAR
                     GTERPTSPAEVRYR"
     gene            54591..55808
                     /locus_tag="PLP50_03685"
     CDS             54591..55808
                     /locus_tag="PLP50_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA50682.1"
                     /translation="MALFESRPAGDARHREAVEECRKRLRADPADGAAALRLADLLAT
                     PDGRAEAIGTLNRVGARLRKAGRIVEAIAVFKKVEALDPRAEVTSSYLSILELKKLQE
                     AEGRSRSSAASAPAESPGDPAATDRRRKSERVHSLRKEVPLLRDIPAFLFELVLEKIR
                     LSSLDAGAILYSEGDPGGTLFFVATGEVETSARGDSGAPVPLELHRTGDVLGVVSFLS
                     GVPHPESAVARTHADLLGLDRAALAPLVKKHREIAEALSRLFAERVLDGVLARSRLFG
                     LLPRRDREAVARRMRPLTVKAGAVVLREGEPGGDAFLVRSGALRVTTRHAGRELALAL
                     LTPHELFGDVASLRHGPRTATVTAVTECELLRLAASDLLAVLAAHPELAPALEEIQLD
                     RFTRNAEKLAKGT"
     gene            55848..57143
                     /locus_tag="PLP50_03690"
     CDS             55848..57143
                     /locus_tag="PLP50_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPA50683.1"
                     /translation="MPAPFPFADRPRLFGVAVSHYQVEGDDPCDWTDWEAAGRTRGGA
                     CGRAAGSWERYEDDAAAAVALGANAFRFSVSWSRVEPRYGTFDEAALERYARLVARLA
                     ALGLEPVVTLLHYTHPRWFHTETPWTSPASVDAFARFAGRVARALGPAARCWTVLNEP
                     FVLVLGGYLDGQVPPGLASGRDAARAFDNLLAAHEAAAAAIREASPGAAIGVAHNMMG
                     FAPARPRNPFDRLLAAAAGRFYNRPLLDAFGEGRWSAFLPPFTRLAGRRPSLPASLDF
                     FGVNFYSRLHLRFGAGGRRIGSFDYRDATGRGLTDNGWEIVPEAFLPLLREAASIGRP
                     LLVTENGLADAADARRAAFLEEHVAVVARAEAEGVPVHGYLHWSLVDNYEWLDGFEPR
                     FGLLALDRETLARRERPSAETFRRLAAEYLGRPAAPRRG"
     gene            57226..>57607
                     /locus_tag="PLP50_03695"
     CDS             57226..>57607
                     /locus_tag="PLP50_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPA50684.1"
                     /translation="MLLGDFHIHTTWSDGRHALPEVVDLFGRSGHDVIAITDHVVNAD
                     SLLGRAAHGFRLSVSRASWPAYRAEIERESRRAWDTYRMLVLPGAELTRNGLTGRSSA
                     HALALGLDEFVSADGPVEEALARAR"
BASE COUNT         7948 a        21274 c        20643 g         7742 t
ORIGIN      
        1 ggagcgtctc ggcgacgagc gtcttgccgg tgccgacgtc gccgatcacg aggaccggcg
       61 cgcgggccga ggcgatccgc tggagggaga cgcgcaggtt ctggatcgcc agggaggagc
      121 cgaccacggc ggtgacgggg tcctcgagcg gcgcggtcac catcgccgcg ggggcgtggg
      181 cgctggtgcg ctccccgaac ggcgcatcgg cgcgctcggc gtggacgaac ccgatcgccg
      241 cggcgagagc gacgccgagg tcgttcagaa gcgggtcggc ggcgagctcc atcggcagga
      301 cgaggaggcc caccgcctcg gcgcccggcc gcgtcaccgg gtagacccgg aggcgcatgc
      361 ccgcttcgaa gatgtcggtc ggtccgccgg aggcgaagac ctcgtcgacc gagcggcggc
      421 ctagccgcgg gagcgtcccc ttccagatcg cggcgatcgc ctctcccgtc gagctgtcgg
      481 ccacggagag gaacgcgacg ctgtggacgg gcgtggagga ggagaggtac gacgcgactt
      541 cctggagcct ctcctccagt gtggccgagg cgcggagccg accgaacagc tccccccaga
      601 cgtcggccag ccggaagcct tgcgccgccg agagcgagct cggacggctc tccgccggcc
      661 gggccgcgtg gaccggctcg tacgtcctcg gccgcggctc gggcacccgc ggggcctggg
      721 ccgcgggcgg ggccggcggg ggcggcggcg gcgcgagcgc ggccgcggcg accgagaagg
      781 cgtccctccc gcgcggcggc ggcgccgcga gacggttcgg gttcggggca ggaccgttca
      841 gcagagtgat cgtcgcctcg ccaatctgga tacggtcgcc cgccgcgagt gcagcggact
      901 tgacgatcgc tccgttcagg tacacgtccg agccgggcgc cgtcccgagg acgtccaggc
      961 ggagctcctc tcccgcgacg gagatgcggg cgtgccggcc gaggacacct cgtgccctca
     1021 cgaccagatc gttcccggac gaagcgccga gatggatcac gtcgcggtga accttgcgga
     1081 tctccgactc gccacccggc ttgcggacct cgaagctgag catgttttcg ggcctccgat
     1141 tcgggagggg gtgcctcgcg aacggacgga aggctaccac ggagacagga cgattgtcct
     1201 cgaagcgtcg acaaaatctg aacgggcccg accctctaga atccctcccc ggtgagacga
     1261 tgccccgccc gctgaccgac gccgttccct tcagcgcccc gcgcgacggc gacctcgagg
     1321 cgctcgtggc gacgctcggc gccgcgctcg ctcccgacgc gttcgagctc gtcctcctct
     1381 gccccgtcac cggccgcaga ttccggaggc ggctcgcggg ggggcgcgat tcgggcctcc
     1441 gacccgcccc ggcctcgcgc aacggcgggc tcccgaacgg caacgtcctc acggtcccgt
     1501 tcggcgagga ggaaggctgg cgcggacgcc tctacctcgt gaggcggcgc ttccgcccct
     1561 ggaccggagc cgagtcgatg cgcttctcgc tcctcgcccc gctcgcctgc cagctcctga
     1621 cgcgcctcgc ggaggcgccc gcggcgcgct gcgcgcgaga acgcctcctc gccctcgccg
     1681 aggcgaccga ggcgccggcg atgatcgtcg accgcggcgg gacggtcctc tacgcgaacg
     1741 gcgcggccga ggagctgctc tcccgcgact ccgacgacgg cccggccgtc ctctccggcg
     1801 accggcggac gatcccgctc gcgtcccggc tcctgcagct cgcggcgcgg gagaacccga
     1861 caacggagcg gatggcgctc accgacggcc gatgcctcga ggcgcggatc gtcgactgcc
     1921 ccggcgacgg cacgtccgcg gccccgccgg cgcgcatcgt cctcctgaag gagcgggccg
     1981 ccccgacgat cgacgacgtg aggccccacc ttcacgcccg cggcgtctcc gaccgggagg
     2041 ccgaggtcgt ctcgggcgtc ctgcgcggcc tgcgcaactc cgagatcgcc ggcgagctct
     2101 tcatcagcga gtacaccgtg aaggaccacc tcaagcacgc gttccgcaag ctgtcggtcg
     2161 cgtcccgcgg ggagctcctc cgcgccctcc acgccgtccc cggccgcccc ccggcgcgtg
     2221 tcgcctgaac ggcggccctt ccgacggccc ttcccggcac gacccccctc cgtcgtggta
     2281 gggtcgcccg cctgacgctg ccatgttccg actgaaaaag ctcgagatcc acggtttcaa
     2341 gtcgttcgtc gaccgaacgt ccctcaccct tccgtcccgc ctcaccgcca tcgtcgggcc
     2401 gaacggctcc ggcaagtcga acatctgcga cgccgtgatg tgggtcctgg gcgagcagtc
     2461 ggcccgtgcc ctgcgcggga cgacgatgga agacgtcatc ttcaacggct ccgcccggcg
     2521 caggcctctc gggatggccg aggtctcgct cacgctcgag tcgcgcggcg ggcagtgggc
     2581 ggacaccggc ggcgaggtcg tcctcacccg ccgcgtcacc cgcgacggga cgagcgacta
     2641 catcctcaac ggccgcaagg cgcgcctgaa ggacatccag gacctcctcc tcggcaccgg
     2701 cctcggcgtc cgcgcctacg cgatcatcga gcagcagaag atcgacctga tcctctcggc
     2761 caagccgcag gaccggcggc gcctcatcga ggaggccgcg gggatctcga agtacaagat
     2821 ccggaagcag caggccgagg tgaagctgga ggagacccgc gcgaacctcc tccgcctcac
     2881 cgacatcgtc tcggagatcg agcgcgcctg cacctcgctc aagcggcagg cctcgcgcgc
     2941 ggcccgctgg aaggagcagg ccgacgtcct ctcgggcctg aagaagcgcc tcctgcgcct
     3001 gaagtccgag cggctcgtcg ccgcgaccga ggccgccgag gcggcgctga agtcgacgac
     3061 cgacgcggaa ggggccgccc tcgccgagtc gggccgcctc gacgccgcct tcgaggaggc
     3121 gcgtcacgcc gccgaggagg ccgagcgcct ccggcgcgag aaggccgacg cgttcgccgc
     3181 cgcccgcgag gcgctcctcg cggccgaggc cgcggtcacg gcggctgccc gcgaggccga
     3241 ggagaccgag gcccgcaggg agctggtcgg ctcgcagcgc accgaggcgg aggaggacca
     3301 cctttcctcc gtcgaggagg cgacgaaggc cgagcagctc ctggccgagg ccgccgagga
     3361 gctcgccgcc gccgaggcga aggcgaagga gacgggcgag gcgctcgccg ccgcgacccg
     3421 tgacgagagc gacgccacca cgcggaggga gggctcccgg cgggagctcc tcgccgccgc
     3481 cgcccgcgcc acggaggcga cgaaccggga gcgcgaggcc gcgcttctcg tcgaccgcct
     3541 cggcttcgcc ctgacgaagg tcgaggagcg gaaggggcgc gccgaactcc agctccagga
     3601 gcgccgcgcc gcgatggagg cggcgcgcga cctcgaggcc gagacgaacg agcgcctcgc
     3661 cgaggcgcgc cggcggctcg aagaggcggg cgtctcacgg aggtccgccg aggagcggct
     3721 cgcctccctc aaggaggagc gcgagcgcgc gacgcgcgcc ttccacgccc tcgacaagcg
     3781 cctcggcgcc ctcgagaccc ttctcgcggc gcgcgaggcg ggcggcgaag cggcccggcg
     3841 ggccctcgag acttccggcg cgaaggccga cggcgtcctc gccgaccact tcgacgccga
     3901 ggaggggttc gagcacgcgc tcgacgccgc gctcggcggc gccctcgaag ccccggtcgt
     3961 ccgcgaccgc gcggcgctcg cctcgctcat tgagacggtc cgcgcctcgc acctcggcac
     4021 ggcgcgcttc gtccacccga tccccgagac gccgttcccc ctcaagcccg cggacccgcg
     4081 ggtcctcggc ctcgcccgcg acctgctgac ggcgcggccc ggcgacgagg cgctctcggc
     4141 cgccctcccc gacgccctcg tcgtcgcttc cgtcgacgac gccctcgccc tcgccgagct
     4201 cctgccggag cggacgttcg tcacgaagga cggcgtcgtc gtccgcggct cgatcgtcga
     4261 ggcggccggc accgaggtgc cgggcgaggg gctcttcacc gtccggcgcg acctgaagaa
     4321 cgtcggcgcc gagaaggaca ccctctccga gcggctcgcc gccctcgagg aggagatctc
     4381 cgggacgggc gcgcaggcgg gcgaggcggc gcaggccgtc gagcgcgaga tcgccgcgtc
     4441 gcgccgcgcc gagcgggagc actccgaggc gctcgcgcgc ctctccgccg cccgcgacga
     4501 ggcgacccgc gccgagcgcg aggtcgcgac gatcctggag gaggagaagc agctccgcgc
     4561 cgacctcctg cgcgtcggcg aggagcgtgc gaaggcgctc gagctctccg cgcagaagac
     4621 cggcgagatg accgccatgg aggccgcaat cgccggcctc gagagcggcc tcgagtcggc
     4681 ccgcgcggtc cgcctctcgg cccagcaggc cgacggcgag gcgcgcgccg cgcgcgacgt
     4741 cctccgcgag cggcggcggg ccgcacagga ggcgttccag acccaccgga accgggccga
     4801 cgccgccgcc cgccgcctcg ccgagctctc ccagcagatc accgctctcg gcgagcggaa
     4861 ggagcgcgcc ctcgccgccg gcgaggaggc gcgcgagcgg cgcgaggagg cggtcgtcgc
     4921 cgcatccgag gccgaggccg ccttgaagca gtccgacgcg gccgcggtgc agcgccgcgg
     4981 cggtctcgag gagctcgacc gcggcgccca tgcggcccgc gaggcgctcg acgccgcgcg
     5041 ccgcgcccgc ttcgaggccg agctcgtcgt cgagcggcgc cgcggcgacc gcgagcacct
     5101 ttcggagacc tgccgcgccg agttcggcgt cccgcccgag gagctcccgc cggtggagcg
     5161 ggcggaaggc gagcccgagc ccgacgacga ggccctcgcc gcgatgcagg aggaggtcgt
     5221 ccagaagacg gtcgccctcg agcggctcgg ccccgtcaac cacgccgcgc tcgaggagtt
     5281 cgacgtcgag agcaagcggc tccaggagat gtcgacgcag aagctcgacc tcgagaagtc
     5341 gctcgaccag atcctcgaga cgatccggac gatcaacctc acgtcctccg agcggttcac
     5401 ccaggcgttc gtcgcgatca acgcgaactt cgcgcaggtc ttccagaagc tcttcggcgg
     5461 cggcaccgcc tcgatgcagc tcctcgacga gagcgacccg ctcgactcgg ggatcgagat
     5521 catggcccag ccccccggca agcggaacca gacgatcggg ctcctctcgg gcggcgagaa
     5581 ggcgctcacc gccgtctcgc tcctcgtctc gatcttccgc tacaagccct cccccttctg
     5641 catcctcgac gaggtcgacg ccccgctcga cgaggcgaac atcgaccgct tcacgacgct
     5701 cctcaacgag ctctccgagg agacgcagtt cgtcctcatc acgcacagca agaggacgat
     5761 ggagaccgcc caggcgctct acggcgtcac gcaggaggag ccgggcgtca gcaagatcgt
     5821 gtcggtgcga ttcgactgac gcggatcagc cgggggtccg gggggcgcgc tcggacgcca
     5881 cggcgggcga ccgtggaaat gtacgaggcc ggcccatctg tcgcagaatc cggacgggga
     5941 ggcagagtgg agatctcctg gttcgtctct cgcgacggtc agccggcagg tccgttctcc
     6001 gaggcagagc tcgtcacggc ggccctggcg ggcgagatcg gacccgacac gctcgtctgg
     6061 cacgagggga tgcccgcctg gacgcggctc atcgacgagc cgtcggcccc ctcgctcccc
     6121 ggcatctcga gacggatgac ggactgctcg gagtgccggc gaagcgtccc tctcgacgag
     6181 acggtcccct tccggggcca caccttctgc gccgcctgca agcccgtcgt cctcatgcgg
     6241 atcgaagagg ggcttccgcc ctgccagggg ccggcgttcg cgcggttcgg gacccgtctt
     6301 tcggcgaggt tcgtcgacat gctcggtctc tggatcgtca ccacggcgat ccagatcccg
     6361 atcgtcggga ttccgctcat cacgcagatc acgccggact cgccgggatt ctgggttcgg
     6421 cagatcgccg ccatgctcgt cggcgtcgcc gggaacgccg cctactacat cgtcctgcac
     6481 ggccgctccg ggcagacgct cggcaagcgg ctcatgcgga tccgggtcgt tcgggccgac
     6541 ggctcgccgc tcgggttcgg aaaggccacg agccggttct tcgccgacat gctctcgtcg
     6601 atgacgctct tcatcggcta cctgatggcc gcgttcgacg aggagcatcg ggccctccac
     6661 gatcgccttt gtgacacgag ggtcgtcgag ctccgatgac cgcatcgctc acctgcggca
     6721 gttgccgatc cccgatcgcc cccgagcgcc tcggcatgga cgcgactctc gcctgcgagg
     6781 cctgcggggc ccttcacctc ggcgaggtct tcccggcgtt ccaccgcgag cgtcccgccg
     6841 gcgcgaccgg cgaggacgtc gaggcggcgg gggaggcgac ctgcttcacc cactcgagga
     6901 agcgcgccgt cggcgcgtgc gggcgctgcg gagccttcgt ctgccggctc tgcgagctgt
     6961 cgatcgccgg agagaggctc tgcccccgtt gcctcgacac gggcgtggcg aaggggacga
     7021 tcccgaggct cgagaccggg cggctcctgc acggccggat cgcgctctcg ctcgcgacgc
     7081 ttccgctcct cgtcttctac ttcacgctcc tgaccgcgcc cgccgccatc ttcgtcgcga
     7141 tccggttctg gaaggcgccg aggagcctcg tccgtccttc gcgcgcgggg cacgtcgtcg
     7201 cggtcgtcct cggtctcgcc cagctcgtgg gctgggcgtt cctggtctcg atgctcttcc
     7261 ggatgctgac cgaagggagg ggcgcgtgat ggtcgagtac cggaagctcc cgggccgcgg
     7321 ccgtcgattc acgagcccgc gctggctcga cgtcatggcg cctcgcacga ccgtctggct
     7381 cggaagcgac cacctcctcg tcgtcgacga caccggctac tccgagcgct acaggcgatt
     7441 cccatacgcg gacatccagg cgatcgtcgt tcgcccgacg aagacctacg ggaccgccgc
     7501 cgccgtcctc gccctgctcg cggccgttcc gctggccggg agcttcctga cgggcgaggt
     7561 cggcgcgtgg atcctccgct cgctgggcgg ggtcttcctg ctcatcctcg ccgtccacgc
     7621 ccttcgggga ccgacctgcc ggacgacgct tcagacggcc gtccacgtcg aggaggtccc
     7681 ctcctggaag cgtctgcgcg tcgcacgccg cgggatcgcg cgtctccggc cgctcctgca
     7741 ggccgcccag aaagagacgg ttcccgcgac gccccctccg acgtgagcgc cccgctcgtc
     7801 ctcccgtgct tccgccaccc cgagcgcgag gcagcggccc gttgccccgc ctgccggcgg
     7861 gcattctgcc gcgagtgcgt gacggagcac gacgggaggg tcctctgtgc gtcgtgcctg
     7921 agggcggcgg cctctccggc ggccccgccg cgccggcgga tcgccgagcg gttcgcagcc
     7981 gcggccggct tcctcgccgc gtggctcttc ttctttctcc tcgggcggct cctcgtcgcg
     8041 ctgccgagct cggccgggga gggggcaggc ttcggtgacc cgccgaccag atcggagcct
     8101 cggtgagacc gaaggcctcc ggagacgacg cgccccgggc cctcgatctg ctggaggacg
     8161 gggcgttcct cctcgccagc gcctcgcccc gcgcgctcct cctctacgcc ggcgggacaa
     8221 ttccgttcct cggggccgcg ctcctcctgg ggttcgacct cgcccgcgcc ggcggtgcct
     8281 cggcctctct gccggccgca tcgttcggtc tcgcgctcct cttcgtcgtg atgaagaccg
     8341 cgcaggcgct cttctgcgaa cgccttcggg tcgaggggct ctcgagcgcg gccctcgaca
     8401 cgccgcccgg cacgctcgcg atcgccgccc gccaggcggc cctccagccc tggagcctcg
     8461 tcctcctgcc gctcgcgctc ctcgcgacgg tcccctacgg actgacggcg gccttcttcc
     8521 agagcgccgt cgccgagggc cttcggggct cgagcctcgc cgatctcgcg gctcgagcgc
     8581 ggcggcacgc gggcgtctgg cagggccaga accacgtgct cctcctggtc cttctcgcgt
     8641 tccgcatctt cgccttcctg aacgtcttcg tcctcctcgt cgtcctcccg ctcctccttc
     8701 ggacgctcgc cggcgtcgaa acggacctct cgcgcgcggc cggacacctc ctctcgctcc
     8761 cgctgctggc cgtctccgcg gcagtcaccc acctcctcgt cgacccgctc gtgaaggcgg
     8821 tctacgtcgt ccgcagcttc cacgtggaat cccggcggac gggcgaagac ctcctcgcgg
     8881 cgctccggcg ccggggcacg gcgatggtcg tcttcctgct cgccgcgctc cccgcgcggg
     8941 ccgcggctcc ctcggtcgac cccgcgagcc tcgaccgcgc tctcgacgag gtcctctccc
     9001 gacgccgctt cgcctggcac ctgccgaagg agcccgccgc cggcggctca ggggtgttcg
     9061 aggacgtcgt tcgggggctc tgggagatgg tgtccggcgc ggcaacggcc gttctccgtc
     9121 tgctcgatcg gctcgcccgg gcgatcgccg agaggctgcg ctgggggcgc gccgagggct
     9181 cggacagagc gtgggccttc gggctggccg gcgagcagct ccttctcctc accgctctgg
     9241 ccgtcgtcgc cggagcgctc gctctcctca tcgtccggat cgtgcgaaag aggcagctcc
     9301 gcgcgccgga gcccgccgcg cccacgccca cgcccgagat cgacctcctc gccgacacgg
     9361 tcgccgccga tgcgaaggag ccggaggagt ggctcgccct gggcgacgag tggatcgcga
     9421 aaggggacgg acggcgcgcg gtgcgggccc tgtacctggc cgggctcggc gcgctcgccc
     9481 gcggcgagct cgtgaggctc gccccgcaca agtcgaacgg cgactactcc cgggagctgg
     9541 agcggcaccc gggcgcgtca ccgagcctgt cgcccctctt cggctctctc gccaccgagt
     9601 tcgatcgcgt cttctacggg acccaccggg tgagcgccgg ttcgctcgcc gctttcaagg
     9661 aggacgtccg tgcactccgg gccctcgcga taccgtagga gctcgggcat cctcgcggcg
     9721 ctcctctgcg cgacggtcgc ggcgggagcg tacttcttcc tggacctcgc tctcgcccgc
     9781 ggcgacgtct accctcccta ttcctcgttc cgaagcgacc ccctcgggac ccgcgccctc
     9841 ctcgaggcgc tcgcggagca gcccggcctc gatgtgagga ggctcctttc tcctcttcca
     9901 gccgagccgg acgccggcgc ggccgtcgtc ttcgtcatcg ggccgacggt cgaggccttc
     9961 gcggaagcga cgacgaagga gctcgaagag gcggaagcct tccttcgggc cgggggacgg
    10021 ctcgtcctcg ctctccgccc cgtcggctcc acggacatcc cggggacggc tcccggcgtc
    10081 gggaaggagc cggcccggcg gccgcgtccg gccgtgggac tcgcgagtcg ctggggggtc
    10141 gcgatccggc gggccgggag cgctccggtc gccgcggccc ggcgggcgcc gggccccgac
    10201 gatcctcatg gctcgcttcc cgaagccctc acgtggcgtt ccgccgcccg gctcgagctc
    10261 ggctcggacc gctggacgac gctgtacgcc atcgacgacg cacccgtcgt cgcgtcgcgg
    10321 cgcttcgggc gggggacgct cgtcctcctg accgacgcct cccgtctcac gaacgcgtcg
    10381 atgcgcaagg agcgcgcgag cgttctcctc gctcacctcg tcggcgagaa gacgacggtc
    10441 ctcttcgccg agacgcacct cggggtcgag gagcgagagg gcgtgatggt cctcctccgc
    10501 cggtaccgtc tcggcggcct cctcctggcc gctctcctcg tcgcgggcct ctgggcgtgg
    10561 cggaacgcga cggggctcct ggcgccgcag gaggcccccg gagcgatccc cggcgcgggc
    10621 gggcgagatt ccttcgaagg gctcgtccgt ctcctcacgc gggggatccc gccctcgcgt
    10681 ctcctcgacg ccgctctcgc ggagtggagc cgaacgtcgg ggacgccgac cggcgagatg
    10741 cgccgtctcg ccgacgaggc ccgccgcacc accgagatcc cggctgcgta cgccgcgctc
    10801 tgccgcgcag cccatcccga agcccgtgcc cgaaaggagc cccatgcctg aacccgtcca
    10861 ggacgtcctc gcccgcgccc gaacggagat cggaagggtc gtcatcggcc aggagcgggc
    10921 cgtcgacttc gccctcgtcg ccgtgctggc gcgtcagcac gccctcgtcg aaggcgtgcc
    10981 gggggtcgcc aagaccctcc tcgtgcggac gctcgcgcag gtgctcggat gcgacttcgc
    11041 gcggatccag ttcacgcccg acctgatgcc cgccgacgtg accggctcga gcgtcttcga
    11101 tctggagcgc cggcagttca ctctggtgag ggggccgatc ttcacgacgt tcctcctcgc
    11161 cgacgaggtg aaccgcgccc cggcgaagac gcaatccgcc ctcctgcagg cgatgcagga
    11221 gcggcaggtg acgatcgacc gggagaccca cccgctctcg ccgagcttca ccgtcttcgc
    11281 gacccagaac ccggtcgagt tcgaagggac ctacccgctt cccgaggcgc agaaggaccg
    11341 cttcctcctg aagatcccga tgtccgttcc gccgcgcggt gacgagctgg cgctcgcgcg
    11401 ccgcacgctc ggtcccgacg cgcccgagga ggtcctcgcg cgaggggagg tccggcccgt
    11461 cctcgggacc ggagagctcg agtccgtccg gcccgagatc gccgcggtcg tcgttcgcga
    11521 cgagctcgtc ggctacgccg tcgacctcgc gcgcgccacg agagaggagg agtcggtcct
    11581 cctcggcgcc ggaccgcgcg ccacacaggg gctcctcctc gccgctcgcg ctctcgcggc
    11641 gatctcggga cgcgacttcg tcaccccgga cgacgtcaaa gcgctggcgg tccccgtcct
    11701 cgagcaccgc ctcgtcctcc gtcccgagtt cgagatcgag gggctcgctc cggccgaggt
    11761 cgtcgagcgg gtcctgcaga gggtccccgt cccgagatga tcgtcccttc ccagcggctc
    11821 ctcgcgttca cgggcgcgac gctcctcccg ctcgcgctgg ccttcgccgc gtcgccggag
    11881 gccgcgccgg ctctcggagg gctggcgctg gtcggagccg ccgtcgccgt cgccgacgcc
    11941 ctcgccggcc cgcgccgcct gcgggccctg cgcgccgcct tgccgcccgt cgtgaggctc
    12001 acgcgggcga agccgggccg cgtggaggtg accgtgggcg gagctccgat cgggggcctc
    12061 cgaatcggcc tgccgctccc atcggagatc gagtgcgaga gcccggaacg acgcgtcgcg
    12121 gagaggaccg cggattcgag cgcggaagat acgaccgtcc cgttcgaggt ggtcccccga
    12181 agaacgggcc gctgggccgt cgccggcctg cgcgccgagg tcgcctctcc gctcggcttc
    12241 tgggacctcc gcacgagcct ccccgcctcg accgagctgc gggtctaccc gaacctcctc
    12301 gcggagaaga aggccgcggc gacgctcttc ctgaaccggg gccgcgccgg ccttcacgtc
    12361 cgtcggcagg tcggaaaggg acgtgagttc gagaagctgc gcgactgggt cgccggcgat
    12421 gcccgtgagg acgtccactg gaaggcgacg gcacgacgcg cgcggctcgt cacgaaagag
    12481 taccggaccg aaacgaccca ggaggtctac gtcgtcgtcg acgcctcgcg gctctccggg
    12541 cgtccggcgc tcgatgccgc gggacagccc ggctcgacga ctctcgaccg gttcgtcgcc
    12601 gccgctctcc tcctcggcgc cgcgacggag cagcaggggg atcggttcgg cctcgtcacg
    12661 ttcgccgatc gcgtcgaggg cttcgtgagg gcagggagcg gccgcgccca cttccgcgcc
    12721 tgccgcgacg tcctggcact cctcgcgccc cgtccggtcg tccccgcctt cgacgagctc
    12781 ctcccgttcc tcgccggtcg cctgtcacgg cggagcctcg tcgtcctcct ctccgagctc
    12841 gacgacgccg ccatcgccga gaccttcctc gccggggcgc gcgtcctcgc gcgacgacac
    12901 ctcgtcctgg ccgtcagcct ccttcccgcg acggcgaggc ccctcttcgg cggggaggat
    12961 cgcgccgcga cggtggacga cgtctacgcg cggctcgcgg gccatctcca gtggcggacg
    13021 ctccggcagc tcgagaggag cctcgcgagg ctcggcgtca cgttcgccct cctcccgcac
    13081 gaatccgtgg ccgccgacct catcgcccgc tacctcgccg tgaagggacg acaggccctg
    13141 tgatcctcga cctgccccgc ttcgtcgcgt ccgcccgacc gcgctggacc gagctcgaga
    13201 ggctcctctc cgcgctcgag gccgagcccg acgccgagcg cgacctcgcg ttcctcacgc
    13261 ggctccactt cctctacgag cgggccgcag ccgacctcgg aaaggtccgg acgttcgcgt
    13321 ccgagcccga gctcgtccgc cacctcgagg cgctcgtcgc gcgcgcctac gccgaggtgc
    13381 acgagacgcg cgaccggagg agccgcctct ccctccggag cctcgccggc atcgtcctcc
    13441 gggacgcgcc acgggccgtg aggcgccatc cgggcgcgct cctgctggcg accctcctca
    13501 ccgtcgcagg gatggcgttc ggcgccctcg cgctcggcgt cgaccccgcc acgaagcggg
    13561 tcctcgtccc cttcgagcac ctctcgggcg acccggccga gagggtccgc ctcgaagaag
    13621 gcggcgagac gcggcacgcg gacgggcatc gcgccgcctt ctcctcgacc ctcgtcacgc
    13681 acaacgtccg cgtcgccgtt ctcgccttcg ccctcggcgg cacggcgggc gcggggacgg
    13741 ccgtcctcct cttccacaac ggcgtcgtcc tcggagcggt cgcgctcgac tacgtgcgcg
    13801 ccgggcagac gcgcttcctg ctcggctggc tcctcccgca cgggaccgtc gagatccccg
    13861 ccttcctcct cgcgggacaa gcgggcctca tcctgggagc gtcgctcttc cgccggagct
    13921 cgcggtcgcc gctctccgcc cgcctgcgca gcgtcctgcc ggacctcgtt gcgatcctcg
    13981 cgctcgtcgt cgcgctcctg gcgtgggctg cgatcgtcga gtcgtacctc tcgcagcacc
    14041 acgagccgac gcttccctac ggcctgaagg ccgccttcgg gctcctcgag gggagtctcc
    14101 tcgcggcctt cctcctccgg gcggggcggg agcgggagga gccgtgagcg ggcgctcgct
    14161 ctcgatcgtc acccccgagg ggatcacgtt ctccctcccc ctcgccggtc ccctcacgcg
    14221 attcctcgcc tggctcgtcg acctcgcggc gatcgcggcg ctgaccgtcc tgtcgcggca
    14281 ggccgtgctc ctcctcggcg cggcgggaga agacgtcctg cgcgcgctca cgatcctcct
    14341 cggcttcgcg gtgacgatcg ggtacggcat ggcgaccgaa tggtggcttc gtggccagac
    14401 cctcggcaag cgcctgctcg gcctgcgcgt cgtcgacgct tcggggctga ggctcaaccc
    14461 gtcgcaggtc gtcgtccgga acctcctgcg cgcgctcgac gccctaccgg tcttctacgc
    14521 cgtcgggggg gcggcctgct tcctctcacg ccggagccag cgcatcggag acctcctggc
    14581 ggggaccgtc gtcatccgaa cgaggcatcc cgcgctcccg gacctggagg gcctctttcc
    14641 cgcggaggcc aacgccctcg ccgcccacgc tctcgtctcg tcgcggctga agcagcggat
    14701 atcgcccgga gaagccgcga tcggactctc cgcgctcgcg agacgtgacc agctcgacgc
    14761 cgacgcgcgg gtcgcgctct tcgccgcgct cgccgagcgc taccgttcgc tcgtgaggct
    14821 cccggaggag gcggtggacg gactctcgga cgagcagctc gtgcggcagg tcgtcgacgt
    14881 cctctaccgc ggcgcgaagc cgtaggcctt cgcccggatc cgagacgccg cctccgatcg
    14941 ccaggacccg ttccgacgtc cgccgacgat cgcgacctgc gtccggaggg acgccggccc
    15001 tggccctccg ctcgtcgctc ggcttcgcga tcgtcggatc gtagactgcg ggggacggga
    15061 catgatccaa tgagacatgc gccgttcgct gctcgttcga gccgctctcc tcgccgctct
    15121 cctctccttc cccgccttcg cgtccgatga cgtcgacgtc cggaagggcg gaagcctctg
    15181 ggcgcgaatc gagaaaagtg ggtcggtccg gatcgacggc gtgatcgtcg gcgagatcga
    15241 ggacgacggg acgatccgcg tcgagggccg gatcgccggc cgcatcgagg aggacgggga
    15301 gatccgcgag gacgggacgg tccggacgcg cgtcgagaag gacgggacgc tccgccggaa
    15361 cgggacggtg atcgggcgga tcgaggaggg cggcgccatc cgccgccacg gcacgatctg
    15421 gggagaagcc gtgccgtgct gctcctcgtt cgacgacctg cgccgcctga cggcgctcct
    15481 cctcttcttc gaccgcagct tcttcgtccg ctgagggagg ccccgggccg gcccggcgcg
    15541 cgtcagtcga tccggaacga cttccgcgcg cgaacggtca gccccggctt cctcgtcttc
    15601 acctcgaccc ggtgccacgt ccgcggccgc tggtcggccg gcgtgaacgt gaggaagtac
    15661 tggttctcga gctcggtcac gacctcgcgg aagacgtcgg ggagagactt gatcgaggag
    15721 atgaaccagg cccgcccgcc cgtctcccgc gcgaggcggt cgagcccccg gaggagcgcg
    15781 cggacctccg cgcttccctg ggccacggtg aagacgctcg cctccgtggc acgcgcgagg
    15841 tcgacgacct ggtcgaccga catccggctc cggttgtcct cgccgtcggt gaagaggacg
    15901 accgcacgcc gtccgctcga ctcccggagc ttccggagcg ccacggcggc ggcgtcgtag
    15961 agcgcggtct ccccgctcac ccgtgcggcc tcgagcgcct ttcgcgccgc ggtgcggtcc
    16021 gtcgagaagg gagtgaagcc gacgacgttc gacgcgaacg tgaggacggc ggcccggtcg
    16081 tccggcttga gagcggcgag gaactcgagc gccgccgcgc gcgcgctccg gagcttgaga
    16141 gccatggagc tcgactggtc gaggacgagg acgaccgaga gcgcggagtt caccgactcg
    16201 aacgtctcga tctcctgcgg ctggccgtcg acgaagacgg agaagtcctc acgcgcgagg
    16261 cccctcacgt aggccccgcc ggagtcggtg acgatcggcg agagcgagac gacccgggcg
    16321 tcggcggtcg cggagaagcc gaggccgcgc gtcctcaccc gggaaaccgc ggcctcgccg
    16381 ccgtcgcggc ggaggacggc ctcgatgcga tgcggacgcg gggcgctccc cgcgctccac
    16441 gtgagcgtcc atggctcgcc gtggatcgtc ccggcgggcc ggccgtcgac gaagacctcg
    16501 acgacgtcgc cggcccggtg ccccgtgagc cggacctcga ccgtcgactc gccgagcgga
    16561 atgtcgtcgg gccccggcct cacgatccgg agggagggtt cctcctgggc ccgcgccggg
    16621 cccggcgcga acgcgacgag gagagcggcg agcgaaagca gaaggcggcg aacgatcgtc
    16681 gtcacggtcc cgggacctcc attcggcgga cgcggccctc cgagagcgcg gcgccgacga
    16741 cagcgtccct ctgcgcgggc cagaaggaac cggacgtcgc cgcgaggggc cgccagtcga
    16801 acgcataagc catgcgcgca cgcctcatca ggtcctcgag cgcggaacgg aggacgagag
    16861 ggacgagcgc ggccggatcg cccgcatcgg agaccgacct cgccacggcg atcggaaggt
    16921 cgtggaggcg gtccccgaag acctgcggca cgcggtactc ggaaaggcgc tcgtaggacg
    16981 gcagcgtgac gtcgcccagg cggctccggc cctcccagtg gaaggccctc ggcccgagct
    17041 cggcgaggag cgcccccgcc ctcgcttcgc cgaggcgctc gacgcgacgg gcccagacct
    17101 cgcgcgcttc ccgcgccgcc ggcacgtcgg gcagcgcgcc gtcggcccgg agcgcgaggc
    17161 gccgaccgat ccgcaagagg tccgacggcg agaggtcgtc ggccgccacg gcggcccctt
    17221 cccgggcgag acggttccgc cgggaggccg ggaggaggtc gcgggcgagc cggctccagg
    17281 cgtcgtgccg ctccgtctcg ggaaggccgg cgagggcgac cggaagctgc tcggcggacc
    17341 ggcaggcgag cgccacgaac cgcagcccct cgtcgtcgag ccgcgccggc gtgaagagga
    17401 cggaggggag gacgagcccg gagcggacgg cgtcgttcga gaggaagccc cccgcgtcgt
    17461 agacgagacc ctccgcgtgc aggaggccca ggggggctcc gattcccgcc cagccccccg
    17521 cgagcctcag gggcgcccct tcaccgggcg tgtcgatccg agccggctgg aacggcgagg
    17581 ggagcacgat gcccttgcgg gggccccacg acagtgagtg cctcttcacc aggagcgaat
    17641 cggcgaacag aaggtcgctc gagtcgagga cgtgccctgc gtagacgtga acggcgagcg
    17701 cctcgagcgt ccgctcggcc cggagcgcgt cgaggcgcgg gaggccggcc tcgaggaggg
    17761 cgaggacggt ctcgcgcgtc gcggcctgga gcgtgccgag gacgtggcgc gcgcccgaag
    17821 ccgtcgccgc gactctctcg tcggcctccc gggccggagg gatcgcgccg aggctctcga
    17881 ggagcgcgct caccgcggca cgagtcgcct ccgcatcccc cgcgcgcgcg gaggcggacg
    17941 cgctctcgcg ctcggcggcg gcgcgggcga ggacccagag cgggacgtgg tcctgggtcc
    18001 gggcgaacgc ccggcgtcgc gcgacggcgt cctccatcgc gtcgtagacg tacgagccgc
    18061 ccctgaaggc cagcgtagcc ggcgggcgga agccggagag ggccgcgaag aggaggtcgt
    18121 cagccgactt cgccggtccc cggtcctcct cgtcctccca cgagagcgac ccggcccgct
    18181 cgccgccgag gaaccgcgtc tccgactcgc ggagcgcacc gagaagctcc ccgtggagcc
    18241 agaggtcgaa gctcccgact cccgccgcct gggcggtccg gggagtcgcg aagagcggct
    18301 gcccgaggag ggacgcgaag aggccgcgcg cgacgtcgtc ggagaccgac ccgcttcgga
    18361 agagcgccga gaggagctcg accgaggcca ggaagaggcc ggcacggagc tcggcgtcac
    18421 gatccgtccc gttcgaatcg agccggttca acgtgaagag gtacctccgg gccgtctccg
    18481 cgctcccgag aggcagatcc tcgagcggcg catagcaggc gaggaagcgg tcgacgccgc
    18541 gggccaggag gagaagagtg gcgggatcgc cgaggtcggg ccggtctctc acgaggctcg
    18601 agacgacgat gaaccgcttc accgccggca acggcccgga gacgccgctc gtctcctccc
    18661 tgaggatcct ctcgagcgcc tcggccgggc cctccgtccg ctcggcggcg gcggcgagga
    18721 gctgctgaag ctccgcctcc ccctccggca tcgaagggct ctcgagagcc tccagccagg
    18781 tgccggcgcc gcccgggagc tcgatctctc ccccggcggc cgacctgagg tagcccgcca
    18841 gctgggtgaa gtcccaggga tcgcgctggc tgttcccgta gctccggccg gtcttcccga
    18901 tggcggagta gaggcgctcg aatcgctcga cggcctcctc gccgccgccc gacgctccga
    18961 gtacgaacgc gcgggccgtc tcctcggaca ggggccggag ggcgtcgatg acgtacgcgg
    19021 cctttcccga ggccgagccg aacaccttca cgacgaagcg cgcgaggtcg gacggcggct
    19081 ctcccacgac cgcctcccag accggctccg ccgcggcgcc tcccggcagc cggatctccc
    19141 cgttacggat ctcgagcgcc tcggggtaga gcgcgaagcc gtcgagcgcc ttctcgtaca
    19201 tgagcttcca gccagcgtag ccgccggcgg gcccgccgac cgcccggagc tcctcgcggg
    19261 tcctcgcgtc cactccgtgc agggccatca tcatccggga cgccgggacg tccgtgacga
    19321 aacgaaggaa ggcgttccgg gcgttcagcc gcttctcgtc gagaccgagc gtctcccgcc
    19381 acgccgagag agagaacggg agctcggccg tgcccgaggc gcactccacg cggacgtctt
    19441 ccccggccgc gagacgctcc aggacgtcca tgacgggcag ccccaggacg tcgaggaagg
    19501 cctggcggcg caccgcctcc gggtcggcga gcggctccgc ctcgaacccc ggtccctcgc
    19561 cccggacccg gtagccgagc cacccgagcg cccggcgtgt cgcagcccac gtctccggct
    19621 tcgtcgagag gaccgcggga cagccatgct tctccgtcca ttccgcgagg tcctccgcaa
    19681 aggaggcgac ggccttccgc ttctcgctct tgttccacgg gtcggccggg ttcgtccccg
    19741 acaggaggac ccggcccacc tcgacgaaga agtccgactc cgtccgacga tctccgaggt
    19801 cgaggagccg acggatgctc gccgtcccgc cggggacgac gacgggctcc gcgaggaggc
    19861 cgctcgcggg gaggaggacg gtcgcgagga gcggaacgag acgtccgacg ggacggcggg
    19921 acggtgatcc gcggcggcgt cggccggggc cggggcctcc gacccggggc gggacggcgg
    19981 caggtgtcat cggcgcgtca ccctcagcgc gttcccgcct gtcgaagggc cgcccgcgcc
    20041 gcggcggacg gtcctccagc cgtcgcggcg accggcggcg ctccgacgcc atggacctcc
    20101 tcgagcgcgg tcgccaccgc gtcgatctcc ccacggagcg tctcgagccc ccctttcagg
    20161 ctctctcgcg tgacgtcggc cgcggcggcg tccgcggcga atgcgcgaag gacctgggcg
    20221 cgcaggctct cgagcgagta gaggatccgc gtcccctcgg cctcgatgcg ggccgcggcc
    20281 gtcgtcagct cggcccggtg gacgagctgg gcgtcgagcg cctcgagcgc cgccgcgagg
    20341 cggctcttca cgacggcgtc gggggcggcc tccgcccgag ccgccagctc cgccctctcg
    20401 ccgacgagcc gctccccctc gtcctcggag agcattccgc gcagctcgcg ctcgcgccgg
    20461 gacagctccc tcgacgccgc ccggagcgcc tcgacggtct gctcgggcct gcgcaggagc
    20521 tcccgaacga ggggtggcgc cccctccagc tcggagagga ggcggttgca gatgccgtcg
    20581 acgcgcgcct cccgggccga taccgcggcc agcgcggggc tctcggcccg ctccggaggc
    20641 ggcggcgtga cgagcggcgc cgggacggct cgctccgccg tcgccccgct cacggcggcg
    20701 ccgcgctcct ctgccgcttc cgcctgacgc ctctcccggc gccggacctt ggcgaagatg
    20761 tcggccgcgc ccatcgcgaa gccgaagccg acgaagaaga accagaagag ctgcggctgg
    20821 gtcaggccga tgagcccggc gacggcggcc gccccggcca ttacccacgc ctggggcagg
    20881 ctcgcctcgt cctcctcccg cagccgcttc tcgaggcgtc tctcctcccg cgtcgggctc
    20941 cggctcgcgc gatcgacctg ggagacgacc tgcccgacgg cgcgggagac ctcgttcgcg
    21001 acgacctcga tcttcccgac gggcatccgc tcgagcgcgc cgagcacccg tcgaacgctc
    21061 tcttccagct gctgctcgat gcctcggccc gaatcggcca tctcgtcctc ccgccagtgg
    21121 gttatacgcc gcgaagcccg aagggttcag gggcccggac gcctctcccg ccgacagagc
    21181 cggcccgtcc ggaatcgccg accgcgtcgc cgcgaagagt cgatggggtc aggtcttgta
    21241 ttgaggcatt cgcgagcgcg aatgcctcaa tacaagacct gaccccaggc tctcccccgg
    21301 gctctccgcc ccgggctctc cggacgacat cctcgctgcg gtaggctgag gagacggagg
    21361 acaccatgat cctgaacccg aagaggctcg gggagctccc actcgatgcg cgctcgcggg
    21421 agatcgtgga gaagacgatc gcgttcttcg aggcgaaggg gaagaagcgc ctgaagcagg
    21481 acgacttcga ccgggtctgg tattcggact tcctccgctt cgtgaaggag gagcggatct
    21541 tcgccgacct cctgactccg ccggagctcg gcgggcccga cgcccgctgg gacacgttcc
    21601 ggaactgcta cgtcaacgag gtcctcggct tctacggtct ctgctactgg tacacgtggc
    21661 aggtctcgat cctcggcctc gggccgctct ggatgagccc gaacgagacg gcgcggagga
    21721 gggccgccga cgcgctcgcg aacgggggga tcttcgcgtt cggcctctcg gagaaggagc
    21781 acggcgccga catctactcg acctcgacga cgctcgcccc gcggggcgac gccttcgtcg
    21841 cgaacggcac gaagtactac atcgggaacg gcaacgaggc cgcgaccgtc tccgtcttcg
    21901 gaaagatggc ggggagcgac gagtacgtct tcttcgcggc cgacccgaag cgccccggct
    21961 accgcctcgt gaggaacgtc gtcgcgagcc agtcgtacgt cgccgagctc gcgctcgagg
    22021 agtaccccgt cgccgcggac gagctcctcc tccgcgggcc cgaggcttgg gacgcctcgc
    22081 tgaacaccgt caacgtcggg aagtacaacc tcggctgggc ctcgatcggc atctgcgagc
    22141 acgccttcca cgaggcgatc ggccacgcgg ccgggcggaa gctctacggg atgtcggtga
    22201 ccgacttccc gcacgtcgcc cggatgttcg tcgacgcctg ggcgcgcctc gtcgccatga
    22261 aggtcttcgc ctttcgcgcc tcggactacc tccgggccgc cacgctcgag gaccggcgct
    22321 acctcctcta caacccgctc gtgaagatga aggtgacgac cgagggggag aaggtgatcg
    22381 acctcctctg ggacgtcatc gccgcgaagg ggttcgagaa ggacatggtc ttcgagatgg
    22441 ccgcgcgcga catccgcgcc ctgccgaagc tcgaggggac ggtccacgtc aacatcgccc
    22501 tcatcgtcaa gttcctcgcg agctggctct tccggcccgc gtctctcccg gaggtgggcc
    22561 gccgcgcgga gccggcgcac gacgccttcc tcttcgcgca gggccccgcc cgcggcctcg
    22621 ggaagatccg cttccacgac tgggcgccgg ccttcgagag atacgcgcga atcccgaacg
    22681 tcgccctctt ccgcgagcag ctcggcgcct tccgcgagct cgtcgcctca gccgcgccga
    22741 ccgaggagca atcgaaggac gtcgacctcc tcctcgccgt cggcgacctc ttcgccctcg
    22801 ccgtctacgc ccacctcgtc ctcgagggtg cggagcacca ctcgatctcc gacgacgtcc
    22861 tcgaccaggc tttcgacgtc ttcgtccgcg acttctcctc ctacgccctg aagctccacg
    22921 agcgctccgg cgcgacggag aggcaggcgg cgatggcgct ccggctcgtg cggcgccccg
    22981 cgttcgacgc gggccggttc gagcggatct ggaagaacga ggtgctctcg ctccgcgaca
    23041 cgtaccggat gagcgagtag gcgcccctca ggcgagccgg acccggaacc gcgtgccgcc
    23101 cccctcgcgc ggtgcgcagc tcacgtcgcc gccgtggcgg cgcgcgacct gccgtgcgat
    23161 ggagaggccg aggcctgccc cgccgtccgg tgcgccgggg agacggaaga agggctcgaa
    23221 gatcctctcg gccaggtcgg gcgggacgcc tggcccgcgg tcgaggacgt cgacctcggc
    23281 cccggaggcc gcgcggcgga cctcgacctc agccgggccg ccgccgccgt gacggcgggc
    23341 gttctcgagg aggttccgga gcatccggcg caggaggcgc gggtcgccgc ggacgaggac
    23401 gacgtccccc gagagggccg cgccggcacg ggcgcactcc tcggcggcga gggccgtcag
    23461 atcgacctcc tcgaagggct ccccggtggc gcccgcctcg aggcggctcg cgacgaggat
    23521 ctcctccacc agcgcatcca gctcgacgac gctccgcgcg acctctccct tcgcggccgg
    23581 ctcgccgtcg aggaggtccg cggcgacgcg caggcgcgcg agcggcgtcc ggagctcgtg
    23641 ggaggcgttc gcgaggagcc gccgctgggc gccgacgagc gcctcgatcc gggccgcggc
    23701 ccggttgaag ctcgcggcga ggagggccac ctcgtccttg ccttcctcgg ggacgcgcga
    23761 tccgaggtcc cccccgccga gcgcttcgac cgactcgcga agccgctcga gcctccgcgt
    23821 gatccttcgc gcgatcggcc aggccgcgag cgcgacggcg agagccgcga ggaggaggaa
    23881 cccgaggaag ccgagggccg ggcgcccgga cgcggcccgc ccggcgacga gccaccgtcc
    23941 gtcgggaaga cggaggacga cggctggacc gacgccggcc gggtgccaag tggcgctccc
    24001 ggcgaagccg gcgggaggcg gagggagcgg ctcgccgaac gcggcgaccc gctcgcgcgc
    24061 cggcgtgaag agcgagagac gggcgcgcgt gcgcgatccc catctctcga gcgcccgctg
    24121 ctggagggcg acgggggcgt ccgggggcgg gaggagctcg cccgcgatct ccgcggtgag
    24181 gccgaggccg ttctcgaccg gctcgcgccc gtagtggaga tgccacgccg ccccggcgag
    24241 gagcgccacg aggacgacgc tcgcgaggac ggcgacgtag atccgtgcgt ggagccgcgc
    24301 gccggggtgg cgggacggcc tcacgcgtcc tggctccgcg cgaagacgta gcccgcgccc
    24361 ctcaccgtca ggatgcgccg cgggctccgc gggtcgtcct cgatcgccgc gcggatccgc
    24421 gcgacgtgga cgtcgatcga gcggtcgaac gcctcgagcg cctctcccct cacgagctcc
    24481 atgagccgct cgcgggagag gacgcgcccg gccctctcgg ccatcgcgag gaggaggctg
    24541 aactggtggg ccgtcatcgg ccgctcctcg ccgtcgaccc ggacgacgcg cgcgtcggag
    24601 tcgacctcga gcctcccgaa gcggagaacc ctgggagccg ccccggcgac cgcggcgggg
    24661 cgcctcagga cggcgcggat ccgggcgagg agctcgcgcg ggtcgaacgg tttgccgagg
    24721 tagtcgtcgg caccgagctc gagcccgacc acgcggtccg tcgggtcgcc ccgggcggtg
    24781 agcatgatga cgggaacggc cgagcccgac gcgcggaggc ggcggcagac gtcgagaccg
    24841 tccgcgtcgg ggagcatcaa gtcgaggagg acgaggtcga agggctcgcg cgcgagccgc
    24901 ccgaacccct cggtcgccgt gccggcggcc gtcacctcga ggccactccg gccgaggtcc
    24961 cgcgcgacca tctcggcgag cctcgcgtcg tcctcgatca tcaggatccg ggtcgccatc
    25021 tcgacatcat cgtccatgcg ggccaccgcg aggcgggtcc cgcgaaagct ccgcggaacc
    25081 cgcctccggc ctgccgggcg gggagatcgc tcagtgcccg aagcgcgagt ggaacgtctc
    25141 gagcttcgcc gcggcttccg cccgctgcgc cggggtgagg acgtccgccg cgtcggcgac
    25201 ggccgccacg aggcgctccg aggcctcctc catcagctgc acgtgacgca cccggtgcgc
    25261 ctcgagggcc gcccggtcga ccttctcggc cgtcaggatc gcgatgacct cctgcttccc
    25321 ggcgacgagc tcatcgtgga ggggcgcgag gtccttcgcg gcggccttcg cgatcgcggc
    25381 gagcttcgcc tgctgctccg gcgtgccgtc gaggtcctcg acgatccaac cggcgatccg
    25441 gtcgatcttc ttctccacct gcgcggggtc catgtgagag caggcgcccg agcggaacgg
    25501 cccgaggccg aacgcctgga gggcgcccgt cgtcaggacc gccgcggccc ccaggaagag
    25561 ggccccgagg aagagggtac ggccgcggcg gcggcgcttc gccggggcgt tcacggaggc
    25621 ttcggggacg atcggggtga tctcgttcat cttcttctcc ttcggcggaa aggctggcgt
    25681 ccttccgtcg cagaggaaga tgacgccgcc tcgttgcgcc ccttcttccg gcaggtgaag
    25741 ttgtgtgaag tcgcgtccgg ccgcgggcga gcaggggggg gtcaggtcta ccatctacac
    25801 tctgcgcgcg cgcagagtgt agatggtaga cctgaccccc tagccgaggc ggcggcgaag
    25861 ggaggcggcg agggcggtcg cttcggggac gcggagggcg agggcgagga gggcgtaggc
    25921 ggcggcgaag gcgccgcaga cgagcgcgcc gaggaggacc gggccgagcg acgcgaacgc
    25981 cagcttcacg ccgaagccgg ccgccgccgc gccgagcgcc gcgccccaga cacggaggga
    26041 gaacgacgcc ggcaggccgg tcttcccgac gcgggcgttc agcgcgcggc gcaggaggag
    26101 catctcgagc cacccggcga ggcccgagcc gagcgtcagc cccgcgaggc cgaggcgggg
    26161 atcgaggccg aggaggcgcg gaagcgcgaa cgcgaggaag gcgccgagcg cggcgctcgc
    26221 cgtgacgcgg agcatcgcga accgaagcgg cgtgcgggtg tcgtggagcg cccagaaggc
    26281 cgaggcgtag agcctcccga gcgtcgaggc gaggaggccg acggaggccg cggcgaggac
    26341 gatccagacg atgcggacgt cctccgcccc gaacgcgccg gtctggaaga cgagggcgac
    26401 gacgcggtcg ccgaggaggg cgaacgcgac ggccgacggg acgacgagga aggcgacccg
    26461 acggagcgcg gcgtcgagcc gctcgcggag cgtctcggcg cccgcctccg aggagagctc
    26521 ggggagctcg gcgctcgaga ccgacatgcc gaagagggag acgggaagga tgtagagcgt
    26581 ctgcgcgtag ccgagcatcg cgaccgcccc gccggggagg aagctcgcga tgacctggtc
    26641 gacccagctc gagacctgcg tcacgccccg gctcccgacg ctcgggagga ggttgcggaa
    26701 gatcgtccgc acgtgcggcg tcccggtgtc gagcgcggga cggagcgtgc cgacgagccg
    26761 cgccacctgc gggagctgga cgaggagctg gaggaggctc ccggcgacgg cgccccacgc
    26821 ggcgaagacg gcgatctcgg ccccggcctt cgtccccgcg aggacgagcg cgacgatgat
    26881 cgcgccgttc cagaggacgg gtgccgcgta cggcaggaag aagcggcggt ggctgttcag
    26941 gatgccgagg cagaaggccg agaggacgag gaggccggtc ccggggaaga ggatgcggac
    27001 gagccgcacc gtcagctcac gtcgctcgcc cgagaagccg ggcgtgaaga ggtcgacgag
    27061 gagcggcgcc gcgaggacgc cgagcgcgga gaggaggacg gcgaggagcg cgaggagcgt
    27121 cccggccgtc cgcgccgtcc tccgcgcctc ctcctcccgt ccctcgccga ggaggcggga
    27181 gtagaccggg atgagcgagg cggagagcga cccctcgccg aagaggttct ggaggacgtt
    27241 cggcgcgcgc agcgccgccc ggagggcgtc ggccgccatc gagctgccga ggtagtgcgc
    27301 gaagacgcgc tcccgcacga agcccgcgag gcggctcagg aggatgccgg ccccgacgag
    27361 gagggcgttc gccgacgagg agcgccgcgc cgcgctcacc gcagcttgag cgtcgagagg
    27421 gccgcgaggg cgaggatccc cgacacgatc cagagcctca cgacgacctt cgtctcggcg
    27481 agcccgttgt aggccatcgc gtggtggagg ggggcgcgcg cgaagagcct ccgcccgagc
    27541 agcttgatcc cgatcttgtc ctgcacctgc gaggtgaacg cctcggcgac gaagaggcct
    27601 ccgaggatga cgaagaggaa ttcctgcttc aggaggacgg acgccacggc gaggctcccg
    27661 ccgatcgaga gcgagccggt gtcgcccatg aagatctggg ccgggtaggc gttgaaccag
    27721 aggaagccga cgcccgcccc cgcgaacgcg caggcgaaga ccgcgagctc gccggcgccg
    27781 gggaggaacg ggtagacgag gtaacgggac cagatcgcgt tcccgaggag gtagccgaag
    27841 acgccgagga ccgcgatcgc gaagaccgac gggacgatcg cgaggccgtc catcccgtcg
    27901 gtgatgttca ccgagttgcc gacgaggagg acgaagagga agacgaacgg gaagtagacc
    27961 cacgggttgt cgaggaccgc ggccttcagg aaggggacgt ggaggcgggc ggcctccgcg
    28021 tccggaagcg ggttccaggg gctcgcgagg aatgcgacga ggatggccgc gaacgccccc
    28081 tggagcatca gcttcctcgc ctcggacatc cccttgtcgc cggacctcgc gcgcatcttc
    28141 gcccggtcgt cgacgaagcc gatcgccccg aaccagacca tgccgaggag gacgaggagg
    28201 gcgtagccgc tcgtcaggtt gcaccagagg agcgaggaga acgccacgac gccgacgagg
    28261 acgacgcccc ccatgaccgg cgtccccgcc ttgtcgaagg ccgacgggat ccccgtctcg
    28321 cggagctggt cgacgaacgc cttccgctgg agccaccaga tcacgcgcgg catgacccac
    28381 agcgtcagca ggaccgccgt cagcgccgcg ccgatggagc gcgccgagag gtactccgcg
    28441 agccggagga acgagagctc ggggaacggc gcgaggagct tgccgatcca gaagatcatc
    28501 ggggcgactc cttgccgcgc gagagcgcct cgaggacgcg gcggtacgag agcttcatct
    28561 cgaactgggg ccggaagtcg gtcgacgtga ggaggaacgc cgggagcgcc gttgcgagcc
    28621 tcgcccggtc gcgcgcctcg ccccgttcga cgagcgcgag gaccccgcga agggccgcgg
    28681 cgcgcacctt ccagaacttg tagtcggaga gccggaggag cgccgggagc gcgtcggcct
    28741 cgccgccgat cgccccgagc gcttcggccg ccgccgccgc cgcctcgagg ttgcggtcgg
    28801 agaggccgcc cacgagcgcg gccacgacgt cgtccctccg ctcgaggcgg ccggcgaagc
    28861 gcgccgccgt ccgcgccgcc tcggctctca cctcccagta cgggtcggcg aagccggcga
    28921 ggagcgcggc ctcgacgccg gcgtcgacga cgccgagccg cccgagcgcg gtgaaggcgt
    28981 tccgccggac gaagccgacc tgcgcgaagt cgcccccgag gagacgctgg aggagcggag
    29041 ccggcgtccg gtcgccgagg agggcgagga ggagcgggac tttctcggtc gccccgagga
    29101 gcccgaggag cttgacgccg aggttccgcg cctcccagcc ctcgttcgcc aggagcgagg
    29161 cggcgcggct ccggaaccag gggaggtcgc gcggctcgac gtgcgcctcg gcgcggtagc
    29221 gccgtccctc cgccgcggcc gccttctcga gccgggagag gagcgcggcg ttcgagacga
    29281 cgtgcggcgc cgggacgagc gtcggctcga tcgtgcccgt gccgcgtccg tggcggacgt
    29341 ggtgcgcgat ctcggcggcg gcgtcggccc cgaggacgtc gcgccccgcc cgcgacatct
    29401 cggcgaggcg atccggatcg tcgatcaggt cgaggacccg gcgcgcgagg agcgccccgt
    29461 cgagctcctc cgcgaggtgc ccgtcgcgga ggaccgtctc ctcgtagagg acctcggcgc
    29521 cgcccgcccg ctcgagggcg cgggcgttcg cgacctggtg gtcgcccggg aggttcgcct
    29581 tcgggacgac gagcgccggg aggcccatcc gcgcgacctc gttgaggctc cccgacccgc
    29641 cacggatgac gacgaggtcg gagacggcgt agacagaggc gatgtcgtgg aagtagctcc
    29701 gcgagacgta gaacgtcgcg atccgctccc gctcctcctt cgagtagcgc gccgcgaggc
    29761 gcgcctccgt gtcggccccc gcgtcgtagc ccccgccctt gaagagcccg gtcccgtgga
    29821 cgacgaagag gcgctccgcg tgcgggagga ggtcgccgag cgcgtccacg aggccgcggt
    29881 tcagcgagcg tgagccctgc gagccgccga agacgaagac gacggtgcgt ccctcgggga
    29941 tcgcgaagtc gagccgggcg cgcgcctcct ccttcgagac gtggccgatc cgctttcgga
    30001 gcgggtagcc ggtgaccgcc ccgttccccg ggaagaggga gagggtctcg gggaaggtga
    30061 cgaagacctt ctcggcgaac cgcccgacga ggcggttcag cttcccgggg acggcgttct
    30121 gctcgtggac gaagacgcgg gaccggagga ggcggagctt cctcaggagc gtcgccgcga
    30181 agatgaccgg ggccgacgcg aagccccccg tcccgacgag gacgtcgggg cggaagcgga
    30241 ggaggacccc ggccgcctga agcgtcccga cgccgaggtc gaggaggaaa cgcacgagcg
    30301 cgggcgaggg gcgcgtgccg ggaaagcccg aggccctcac gaagcgaatc gggatcccct
    30361 ccttcgggac gacctgctcc tcggcccgcc cccgcacgcc gacgaagagc gtctcgctcc
    30421 cctcctcggc cagggccgct ccgatggcga gcgccgggtt cacgtgcccg gcggtgccgc
    30481 ctcccgtcag gagtatccgg gtgcctctct tcaagccgcg tgcctccgcc cgattctggg
    30541 gcattccggc cgccggggag gtccggctct ccccgcgtgc cgagcccggc attctccccc
    30601 gtttccggcc ttcgtccgcc cctccgcgga tagactcgcc ggccgatgct ctccatccac
    30661 ctgctcgaag acgggaaggg gctccagctc ctcgaagaca cgcctctcgc cagcgcctgg
    30721 cccggcctcg cggggcggtc gatctgggtc gacgtcgagg acccgaccga cgccgaggcg
    30781 cgcgcgctcg cggacgtctg cggcttccac cacctcaccg tcgaggacgc gctgaccccc
    30841 gtccagcctc cgaaggtgga ggagttcgag gggtacctct tcctgctctt ccgcggcctg
    30901 cggccgaccg acgaagggtt cgagctcgag agctacaagc tggccgtata cctcggtccc
    30961 agcttcctcg tcaccgtcca caggcgtccg gtcgccgcgt gccgggaggt cagcaggctc
    31021 gtcctcgagc gggacctgcc gatggagagc cagatggacc gggtcctcca cgctctcgtc
    31081 gacaggatga tggacgagat cctcccgatc ctggaccggt tcgaagagcg cctcgaggag
    31141 ctcgaggagg ccgtcttctc gcgctctccc caccgggagg tcctccagga gctgctcggg
    31201 atgaagcgcc agctcctgac gatccgccgg ctcgtcgcgc cccagcgcga gatgctgggc
    31261 cgcctcgcgc gccgggacct cccgttcatc gaggagcgga cctcgcacta cttccgcgac
    31321 gtctacgacc acgtggcccg gatggaggag acgacgatgg tcctgacgga cctcgcgagc
    31381 ggcgccctcg aggcgcagct ctccgtcgcc tcgaaccggc tgaacgagat cgtcaaggtc
    31441 ctgacgatct tctccgcgat ctggatgccg ctcacgttcc tcgccggcgt ctacgggatg
    31501 aacttcgagt tcctccccga gctgaagatc cggtggttct atccggcgct ctggggtgcc
    31561 tggatcgcga tcgcggccgg catgctcgcg gtctttcgct ggcgccgctg gatctaggac
    31621 ggccttccgg tttcccgctc cgcggacccg gcccgcccgc atcggaaggg cgcgaccgga
    31681 cccaccggcc gagcgtagaa ttttcgccgg gcgtcggagg tgactcgtgc cgtttggggg
    31741 ctcccggtcg tgagcggcct atgcgcaatt ggacggcacg gaggcccgcg gccatcggtc
    31801 ctccgatgcg ccgccctcgc gctcgcgctg ctcggcgcgc cccccgccac ggcgcaggag
    31861 aagagcctcg acgacctcct cagcctcagc ctcgaggacc tcgccgcgat cgaggtcgtc
    31921 tcggccgcga aggtccccgc gaccgccgcc cgcgtcccgg ccaccgtccg ggtcatcacg
    31981 gccgagacga tcgcagagaa cggctacctc acgctcgagg acgccctcgc cgacctcccg
    32041 gggtttcagc tccggaacat cctcggcttc aacgcctacg cattcctcag gggcgtgccg
    32101 agccagaaca acgcgatcct cctcctcgtc gacggcatcc aggtcaacga gctgaactcc
    32161 ggcggcttct acggaggcgg ccagctgaac ctcgcgaacg tcgagcggat cgaggtcgtc
    32221 tacggcccgg cgtccgccct ctacggcacg aatgcgctct cgggcatcgt gaacgtgatc
    32281 cggaaggacc cggcgaaggg aacgggaggg aagctgaacg cctccctcgg cagcctcgac
    32341 acctctctcc tcgacgcccg gtacgcgtgg acctcgcccg ggggcgacgt ctccgtctcg
    32401 gtcgccggga tgcgcaagcg gaccgacaag gccgacctgc gcggcgcgcg gggagacggg
    32461 aactggaccg accaggcgga caactacgag gacgacctct cgttcgacgg cctcgtccgc
    32521 tggaagggac tgagcgtcgg tttcctcgtc caggacaagg aggcctcccg cgcgagcgtc
    32581 cagaggacgg ccgacacgac gttcctcgac cacggtgtcg gctggcacat ccgcttcctg
    32641 aacgcctggg cccgctactc cctgaaggag cgcggtccct ggtcggcgca ttcgactctc
    32701 tacgtccgga gcagcacggt catggacgac acggtgccgg tcatcgaaag ggccacggcc
    32761 tcctcgcccg gcagccagca gcgctggtac cggccgggag agctggtcgg tgccgagacg
    32821 caggcgacct gggcgccgac gaagcggttc tcgatctcgg ccggtctcgt cctggagcgg
    32881 gaacggctct cggagagcta ctcgatcacg acgagcggct cggagctcga acccgcccct
    32941 cccccgcctc agccggagcg gatcacgaac gacctcgcca gcgtctacgt cgaagcgcag
    33001 gccggcctca cgggggagct ccgcctcttc gccggccttc gccacgacga cgcgaccagc
    33061 tacgggaacg tcgacacacc ccgggccggg ctcgtcctga accgcggccg cctcacggcc
    33121 aagctcctct acatggaggc gttccgagcg ccgaagccgt gggacctgac cgacgggctc
    33181 ggaaaccccg acctccgtcc cgaggagatg cggatggtcg aggcatcggc cgcgttctcc
    33241 ttctcccccc acctccaggc cgacgtctcc gtctacagca gccggctcga cgcgctcctg
    33301 acccggtcgg gcgaggggga cgagtggcgg tgggtgaacg ccggggagct cgagacgcgg
    33361 ggggtcgagg tctcggcgga gctccggaag gggcggttcg tggcctgggc gaacgccacc
    33421 ttctgcgagt cggaggacga gacggggcac caggtggcgg agatcgcccg gtcgggcgcc
    33481 aacgccggcg tgaggtacct cgtcgcgcgc ggcgcgacgc tcggtctccg gggaagctgg
    33541 ctcggctccc gcaggaaccc gaaggtcgtc ccggccaccg aagacgaccg gatcgaggac
    33601 gccctcgtcc tccacgcctc ggcctccgtc gccctgggcc acggcctcga agcgcgcctc
    33661 ttcgtcgaca acctcggcgg gacgacgtac gaccacccct cgaacctccc cccgagccgg
    33721 taccgccagc cggggaggac cttccgagtc caggcgggat ggacgttctg acgcgcgcga
    33781 ccgttccgcg gccggcgcgc ctgcccgcgc gccggctcgt ccgcgccctc acccgggcgc
    33841 tgcggaacgc gttcgtcgcc gcgctcctgg tgccggcggc ctccgcggcg gcccaggacc
    33901 ggggtctcga ggcgcgggtc aaggcggcgt acgtctacaa cttcctgacg ttcgtcgaac
    33961 gggagagggc cgagccggtg ccggcagggg ccccggtgcg catctgtgtc ctcggcgacg
    34021 aggcgatcct cgcggagctg aaaggcctca cgggccgcga ggttcgcggc cgcaagctcg
    34081 aggtcgtctc cggacccgag gccggccctc cgccctcctg ccatctcctg gtcatcggcc
    34141 gctccgaggc gggccggctc ccggagatcc tctcggaggt ctcgggcgcg ggcgtcctga
    34201 ccgtcagcga catcccccgg ttcgcccgcg gcggcgggat gatcggcttc cggacggaag
    34261 ggggccgcgt gaggatcgag gtgaacccgg ccgcggccgc cgcggccggc ctcaaggtga
    34321 gcgcgaagct cctcgaggtc gcgcgggtcg tcccccggga gcgtccgtga gcttccggcg
    34381 cgtcggctcc atccgtgcca agctgatgct catcggcatg gcgacgagcc tctgctcgct
    34441 cctcctcgcc gccgccgcct tcggcctcgt cgaccggtac cgcgaccgga agggcctcgt
    34501 cgaggacgtc tcgacgatcg cccgtctggt ggcggaccgc agcaccgccg ccctcgcgtt
    34561 cgacgacccc gggctcgccg cggagaacct cgccgccctg cgtatcaagc cctcagtcgt
    34621 cgcggcctgc acctacgccg gggacggttc cgtcttcgcc gagtaccgct ccggccggga
    34681 ggagtaccgg ttcccgcgag ccgaggacgg accgtcgcac cgcttcgagg aggggcacct
    34741 ctccctgttc gagccggtcg tcctcgacgg gacgaggatc ggcacggttc acgtgcgcgc
    34801 gagcctgcgg gagctcgcgg tcctccgccg ggagtacctc ctctcggcca cggcgatcgt
    34861 cgccttcggc gcgctcgtgg ccttcgccct gtcgacgcgc ctgcagcgcc tcgtctccgc
    34921 tccgctggcc cggctcaccg agacggcccg gcacatcgcg acggagaagg actactccgt
    34981 ccgcgcgccc gcggagggga aggacgagat cgcctcgctc gcgcggacat tcaacgagat
    35041 ggtcggcgtc gtcgaggcgg agagcgccgc cagcgcccgt gcccaggccg cgctccgcgc
    35101 ctccgaggcc cggatgcggc agatcgtcga cctcgtcccc cacatgatct tcgtgaagga
    35161 ccacgaaggg cggttcctcc tcgcgaacca gacggtcgcg gaggcgtacg gcaagaccgt
    35221 ccccgccctc gtcggccggc cgcaggccga gctcgggctc gacgcggagc aggtccgcgg
    35281 atggcgggag gaggaccgga aggtcatcga ggagggcgtc gtctgcttcg tggacgaggc
    35341 gccgttccgg gacgcgtcgg gcactctccg cttcctccag acgacgaagg tcccgctcct
    35401 cctcgaggac ggcgtcaccc gggcggtgct cggcgtcgga atcgacatca ccgagcggaa
    35461 gaaggcggac gacgagctga ggaggtacca cgaccacctc gaggacctcg tgaaggagcg
    35521 gacctccgag ctcgccgtcg cgaaggagaa ggcggaggag tccgaccggc tgaagtccgc
    35581 gttcctcgcc accatgtccc acgagctccg caccccgctg aactcgatca tcggcttctc
    35641 gggcgtcctg ctccagggcc tcgcgggccc tctgaacgag gagcagcaga agcagatggg
    35701 aatggtgtgc cggagcgccg agcacctcct cgcgctgatc aacgacgtcc tcgacatctc
    35761 caagatcgag tcgggccagc tcacgttctc catcgagacc ttcgacctga gagcctcgct
    35821 cgcgagcctg gtccgctccg cggcccccct ggcggagaag aggggactct cgctcgagca
    35881 cgagatctcg ccgtcggtcg gagaggtcac gaccgaccgt cgacgcgtcg agcagatcgt
    35941 cctgaacctc ctctcgaacg cgatcaagtt cacggagcgc ggctcggtgc ggctcgtcgt
    36001 gaccgccgga gacgggcgcg cgacgatccg cgtcgtcgac accggcatcg ggatccgggc
    36061 cgaggacctc gacaagctct tccggccgtt ccggcaggtc gacgccggca cctcccgcca
    36121 ctacgaaggg accggactcg ggctctccat cagccggaag ctcgccgagc tcctcggagg
    36181 tagcatcgac gtcgcgagcg agccgggccg cggaagcacg ttcacggtcg tcctcccgct
    36241 gcgaagggag accccatgaa ggcgaagatt ctgtacatcg aggacaacac ccagaacttc
    36301 tacctcgtga gcttcatcct gaccgccgcg gggtgtgagg tggtctgggc caaggacggc
    36361 cccgagggga tcgagcaggc cgcccggggc tgggccgacc tgatcctcct cgacatccag
    36421 ctccccggca tggacggcta cgccgtggcg cgggccctcc gcgaacgccc cgccctctcc
    36481 ctcatcccga tcgtggccct cacgtcctac gcgatggccg gcgaccgcga gaaggccctc
    36541 gcggcgggct gcaccagcta catcgagaag ccgatcgacc ccgcgaagtt cgccgcccag
    36601 gtggagcagc acctcctcga cgcgcgggcg aagggcgagg gttcgtgagc cgcgtcctga
    36661 tcgtcgacga catcgtcgag aaccggtacc tcctcgagac catcctgaag gccgggggat
    36721 acgacgtcgt ctcggccgtg aacggggccg aggcgctcgc cgcggccgag gccgctccgc
    36781 ccgacctcct cgtcaccgac atcctgatgc cggtgatgga cggcttcgag ctctgccgca
    36841 ggtggaaggc ggacgagacg ctccgcggga tcccgctcgt cttctacacc gcgacctaca
    36901 ccgacgcgaa ggacgaggtc ctcgcccgaa cgctcggcgc cgacctcttc ctcgtcaagc
    36961 cgatgccgcc ggacgcgctg ctcgaggcga tcgggaagac cctcgcctcg ccgcggaccg
    37021 agtctccggc ggcggacgcc ggaaaggccc ttcagggcta cagcgaggcc ctgttccgga
    37081 agctccagag gaaggtgggt cagctcgagg cggaggtcgc gacgcgaaag cgggccgagg
    37141 aggaggtccg ggcgctgaac gccgggctcg aggagcgcgt ccggagccgg accgccgagc
    37201 tcgagacgag catccgcgag atggaggcgt tctcgtacac ggtctcccac gacctgcgcg
    37261 ctcctctccg ggccatcgac ggctacgccg cgctcctcga ctcggaggcg gcggagcgcc
    37321 tcgccccgga gtcgcgcgag ctgctccgga ggatccgggc gagcgcccgg acgatgtcga
    37381 ccctcatcga cgagctcctc gagttctcgc ggacgggccg caccgagctc cgccgcaccc
    37441 gcctcgacat gggcgagatc ttccgctccg ccttcgagca gctgacgacg cccgcggagc
    37501 gggagagggt ggagcttcgc gtcgacgccc tccctcccgc cgacggcgac cccgcgctcg
    37561 tccggcacgt cgtgacgaac ctcctctcga acgccctgaa gttctcctcg ggccgcgcgc
    37621 gcccggtcgt cgagatcggg agccgggagg aggggagccg cgtcgtctac ttcgtgaagg
    37681 acaacggggc cgggttcgac atgaggtacg tcgacaagct cttcggcgtc ttccagcgcc
    37741 tccacagccc cgccgagttc ccgggcgtcg gggtcgggct cgcgatcgtc cagcggatcg
    37801 tggcgcggca cggcgggacc gtctccgcct cgggccggat cggcgaggga gccgagttca
    37861 cgttctcgct ctgaggtccc cggctcccct ccccggcgtc gccgccgctc ctaatccccg
    37921 ctcccgacga cctccccgaa ggtgccgagc tcctcgcgga tcgccgcggc gtcgccgacg
    37981 gcgacgatcc gggccgaccc ggtcccgaag acccggcgcg agacgcggcg gacgtcgccc
    38041 ttcgtcacgg cctcgagtcc cggaacgtat cggccgatct cgtcgggaga aaggccgagg
    38101 acccagaggc gtgccagctc gtcggcgatg ccggccgccg tctggaggga gatcgcgtgc
    38161 gagcccgcgt cccggcggaa ggcgcgctcc atctcctcgt ccgtcggatc cgtcgtcgcc
    38221 atccgctgca tctcgtagag gacctcgccc agggcggccc ccgtcacctc ggtccgcacg
    38281 gccgcgaagg agcgcaccgt gccgcagccg gtcagccagc tcgcgccggc gccgggcgag
    38341 taggtgtagc ccttcgtctc gcggaggttc tggacgaggc gggaggtgaa ggagccgccg
    38401 tagatcgtca tggcgagctc gagcgcgtac gcgtcggggt cggtcctcgg gaggccgagg
    38461 ttccccacga ggaggttcgt ctggacggag cccgggcggt cgaggaggag gatccgcctc
    38521 gggcccggcg tcgcggtcgg agacgcgccg gacgcgggcg ccgtcccctc cgggacccag
    38581 ccgccgaacg cgaccccggc cgaggcgatc gtcgcgtccg gctccacgtc tcccgccacg
    38641 aggaggagcg ccctctccgg ccggaggcgt cgccgcgcct cggccgcgag aagctcgggc
    38701 gtgacggctt cgatcgacgc cgccgtcggc gagatcgtcg cgtacggatg agcgccgtag
    38761 acctcgcgcg cgaacgcccg gacggccagg aacgacggct ccgactcgct cgtctcgagc
    38821 tcctcgtgcg cgaggtgccg cgcgcgcgcg actccgtccg ccgggaacgc ggagcggcgg
    38881 acgacgtcgg cgacgaggtc gaggaggagc ggcagccgcg cggcgaggcc gtaggcgccg
    38941 atcgtgagcg cgtcgggccc cgcgcccgcc gagaggtctc ccccggcttc ctgcgccagg
    39001 tcggcgagct cggcgccgct gcgcgaggcc gtcccctcgc ggagaccggc cgcgaggagg
    39061 cgggcgagac ccgggagggc acgggggtcg tccgaaaggc cgccccgcac gacgagtcgg
    39121 gcgctcgcga gcgggacccc tcgccagggg acgacctgga ccgcgaggcc gttcggcagg
    39181 agcgcggcca ccacgcgggg cgttgggacg ggggcttccg ggcgaagggg cgggaggccg
    39241 gcgagctcgt tcaacgctcc cccttcgggg ggaccttcgc gatcgccgcc cgccgctcgg
    39301 gcgcgagcca ctccgccgct cgccgcaggt cgtccgaggt gaccgcttcg acgagtcgcg
    39361 ggaggtcgag gagcgcctcg ggattcccgg cgatcgccgt cgcctgggcc agctcgatcg
    39421 cgaggccgag acgaggctcg aggccggccc aggtgtcggc gaggaccttc gtcttcacgc
    39481 gggcgagctc ggccgcctcg accccgtcgc gggcgatccg tgcgacctcc tcgtcgaagg
    39541 cggcgaggac cgactcggca gggacctcgc gccggaaccc gacgcgagag aggaagagcg
    39601 tcgtcccctc gtaccagacg cccccctgga acgggttgaa gccgccggag agctccgtcg
    39661 cgatctcgcg cccctcgacg aggccgcggt agaggcgcga ggcacgcccg ttgtggagga
    39721 gctctccgag cagcatcagc ggggcggcgt cgggccgcac ccgctcgggc atgcgccacc
    39781 ccatcgcgag agccggcgtc cgcgcgagcg gcgcctccct cacgaggagg acgggctccg
    39841 cgcgcggcgg ctccgacagg tcgggcctcc cacacgccgc gcctcgcggg atcgggccga
    39901 agtgtctctc cgccagcgcg aagacgtcgg ccgcccccac gtcccccgcg acggcgagga
    39961 cggcgttgtc ggggcggtag aacgccgcga agaagctctc cacgtcggcg agagtcgccg
    40021 cgtcgaggtc gtggaagtcg ccgtagccgt cgtgcgcgtt ctcccacttc tcgaagaggg
    40081 ccagcgggag ctcgccgtac tcgaagaggc cgtacgggtc gtcccgcacg ttgacgagga
    40141 tctcctcctt caccacgtcg cgctggttgt cgagggactc ctgcccgaac gagaggcccg
    40201 acatccggtc ggcctccagg aagaggatcc tctcgagggc ggacgccggc gcgtcgacga
    40261 cgtacagcgt cacgtcgggg cgcgtctgcc cgttgttgct cccgcccgat ccctcgcaga
    40321 cgcggtcgaa gacccccttt cccgcccgcg gcgtcccctc gaacatgagg tgctcgaaga
    40381 ggtgcgcgaa gccggaccgg ccccgcggct cgagccgcga cccgatcctg tagaggacgc
    40441 agagcccgaa gaccggggcc gaccggttct ccgagacgac gacggtcagc ccgttcggga
    40501 ggacgagggt ctcgatcggg agctgcagcg tcaggccttc aggagctgcc gggcgatgac
    40561 catccgctgg atctccgagg tcccttcgcc gatcgtgcag agcttgacgt cgcggtagaa
    40621 cttctcggcc cggaagtcct tcgtgaagcc gtagccgccg tggatctgga cggcgaggtt
    40681 cgcgacgcgg accgagacct cgcccgcgta gagcttgcac atcgccgact ccttcgtgac
    40741 cttcatcccg cggtccttca tccacccggc gcgccaggtg aggaggcggg cggcgtcgat
    40801 ctcggtcgcc atgtcggcga ggtagttctg gatcgactgg aactcggaga tcggcttgcc
    40861 gaactggacg cgctggcggg agtagtcgag ggcgcagtcg aacgccccct gcgccatgcc
    40921 gagcgcgagc gccgcgatgg agatccgccc gccgtcgagg acctgcatcg cctggctgaa
    40981 ccccgtgccg agcgggccga ggaggttctc attcgggatg cggcagtcct ccatgacgag
    41041 ggtcgccgtg tccgacgcgc ggaggcccag cttgttctcc ttcttcccgg cgcggatccc
    41101 cttctggtgg aggtcgacga cgaaggccga gatgccgtgc gtcccggcgg ccttgtccgt
    41161 gacggccatc aggaccgcga cgtcgccgac ggagccgtgc gtgatgaagt tcttcgagcc
    41221 gttcaggacc cactcgctcc cgtcgaggac ggcggtcgtc cgggtcccct tggcgtcgct
    41281 ccccgcctcg gcctccgtca ggccccacgc gccgatcagc ttcccttgcg cgagcgggac
    41341 gacccagcgg cgcttctgct cctccgtccc gaacatgtag aggtggttcg tgcagaggct
    41401 gttgtgggcc gcgatcgaga tgccgacgga gccgtcgacg cgggagagct cctcgatgac
    41461 accgacgtag tcgatgtacg agagccccgc cccgccgtac tcctcgggga tgatcacgcc
    41521 gagcatcccg agctcgccga ggcgcgcgat cgcctcgcgc gggaagacct gggcctcgtc
    41581 ccattccatg acgcgaggcc tcagctcgct ctcggcgaac tcgcggacca tcttccggac
    41641 ggcgagctgg tcgtcggaca gctggaaccc gaacgggacg gaagcggcgg cgccgacggc
    41701 cgcggggctg tcgagaaacg tggtcatcgc gggactcctt gggctcgtcg cccgtgtgcc
    41761 cggattctag ccgcggctcc gtgaaggccg ggtcagcggg ccggcggggg ctctccaccc
    41821 gtggcgggcc gtgcttccgg cgagccgggc cgctccggct cgggcggtgg tggccaggcg
    41881 ccgagcgccg tcccggggta gtacgcggcc aggatctcgg cgtgagtccg gccggcgagg
    41941 gccatgccga acgtcccgtt ctggcagagg ccgacgccgt gtccccagcc gcggccgtag
    42001 aacgtgtgga cgggcgcgcc ggatccgtct ttccccgtga cgtgggtgaa gagcgactcc
    42061 gggagcgcga gcgcgaagcg gatctcgagc ccggagaggt cgaacgactc ccggtccgtc
    42121 gtcacgcgca ccttcgccgc gcgccccgag accccccgcg cgaggacgtc gacccttcgc
    42181 acgacgctcc ccatccgcga ggcgtcgcgc tcgaggagct tgcgcatcag ctcggcgccg
    42241 gtgaagcggc ggacccagtg gatccacgcc gattcgcgct cgaagagccc gtcgaccgcc
    42301 gggacctgcg cgaggacggc gagcaccttc ccgtcgcggg acagcaggcg gacccggtct
    42361 ccgggcgcga cgtcgagcga ggaggccggc gtccacgcct ccccgcctcc ccggaagagg
    42421 agcgcgtcgg gagggagcgt ccgctcctcc cgccccttcg acgtcttcag gacgaccttc
    42481 ccccgaggcg cgccgaccag ccgcccctcc agctcctcga gccctcccgc cctcaggagg
    42541 agggaggccc agaggcccgc cgcctcgtgg ggcgtgaagg agcgatgcgt cgggagcggc
    42601 gtcggtcccg cgagctggaa ccgggagaga gccgtgtgga ccgtccgcgc cggctcgccc
    42661 cagcgcttcg gagccgcgtc ccgttcggcc acctcggtca ggaggcgagg atcgccgaga
    42721 acccccgaga gattggcgta gacggcttcc ggcgcgagcg tcgccggcgg cgctccggcc
    42781 gggaggccga ggcggtccgt gaggagaccg cgcgcgacct tcaggtcggt ccaggaggcg
    42841 ccgcgtcggc ccgcggcgtg gaggagcgcg gcaccgcgga cggcgagggc gccgaagtcg
    42901 tggcccgcga cgggcgacgt ggacgagaaa gcgatcctct cctcgccgcg gcacgcgacc
    42961 gaccggaggt acgggaaggc cgccggcgcg taggacggga agacgacgac ggcgtcctcc
    43021 gtccgccctc cgcaggtcga ggtgaagagg gtgtcggccg ggacgccgtt ccagaggagg
    43081 acctgcccgg ccgtcgcgcg gaccgcctcg ctcgagagcg gctgctcggc gctcgcgccg
    43141 ccgtagacct ggcatcgggc ggtcgcgcag aggtcgtacc cttcggccgc gaagtcgccc
    43201 cgccgcttca cggcgtaggt gcgcgccgcc acggcctgcg ccttcagggc ctcgagctcg
    43261 tcgtagacgc gcggccccat ctccagcggg acgacgccga ggaggtactc ctcgaggccg
    43321 acccggttga cgacgtggag cgtcccgcgg ggtgtcgcga cgaggaggag cgttccccgg
    43381 tagggcgctc cgttcacggg caggagcgtc gcgtccgcgg ccgcgagctc gaccggcgac
    43441 gggacttcga gccgcccgcc cggaacgtcg acgaggagcg tccggtccgg ccggcccgga
    43501 tcggccaggc cgacgaggaa cgcctcgggg acggcgtcgc gtcggactcg atagaggagc
    43561 gggtcgagag aggcgcgatc gccgggcgga ccgaggcgca cggcgaaacg gccccctccc
    43621 tccgcgacgg cggccggcag gccgaggagc gccgcgaggc ggaccgcttc ggcctcggcc
    43681 gtcacccgcg cggtaaacga cccggcctgg acctggaacc gaggcgcagc gtcggcctcc
    43741 gaggccgccg gccgcgcctc gagcgccccg acgacgcgcc ggacctcgcc tcccgagcgg
    43801 aggagccact cgcccgcggg aagccggaag gcggcgcggt cgacggcgac gcccacgtcg
    43861 aggcgcggct cggggacgtc ggggaaggcg gtccgcacgg acggaggggg cgtcggggcc
    43921 gtcgtcggag cgggcgtggg cggcggggac gggacgacgg tgacggggac cggccggggc
    43981 gtgcaggagg cggcgagaag ggccgccgcg agcgcggccg cgagcgagga ggtccgcgtc
    44041 atcgggtcag gcgaacgcgc gggcgccgtg cgccctccgg aaatcgtcgg cgctgatcgg
    44101 cacgagctgc gtcatctccc agagccggct ccgcacggga gcgccgacgc gcacctcgag
    44161 cgtctcgccg ccgcgggcgt ccggctcgtc cgcgaagttc gtcgtcagga tcgtcagccg
    44221 cctgcggttg tagcgcgtgt tcaggagacc gtagaggatc tcgcggaccc agtcggacgg
    44281 gcggcgcgcc ccgagctcgt cgaggacgag gaggtccgtg tcccggtacg gggcgacgat
    44341 gtcgtcctgg ctctcgtctc ctcccttccc gaagctcgcc tggatccggt cgaggaggtc
    44401 ggagaagtcg cagaagaggg ccgaggcccc tttcgtgtcg acgagctcgt tcaggatcgc
    44461 gacggcgagg tgcgtcttgc cgacgccgac cggacccgtg atgaggagcc cggcgtcgac
    44521 ggcgggccac tcctcgaccc agcgcttcgc gaccgccttc gcccgcttca gcgactcgtt
    44581 gcggaggtcc ttgaagttct gaagccggca gttccggtag cgctcgggga tccgcgcccc
    44641 cgcgcggcgc gccctcgcga tctcctccgc ccggcattcg caggggacgg tggagccgtc
    44701 ggcgcgctcg agcctcccga aaccacggca gaccggacag ggaacggaaa cggcgggctc
    44761 gtccacgcgg acgattgtag ggggcctctc tcccgacgtc agcggaggac ggaaaggagc
    44821 cggcgcgcct tcacgccgcg ccgcaggcgc tgaagggcct gggcctcgat ctgccggacc
    44881 cgctcccggg agaggtcgag ctcgagcccg atctcctgga gcgtcatcgg ctcgcgctcc
    44941 ccctcgctcc cgtcctccgg gatcccgaag cgacggcaga ggaccgccct ctccttcggc
    45001 ttgagctcgc cgaggaactc gcgcgcctgc tccgccgacg cccggcgcag catgtcgtcg
    45061 tctgccgagg agaccatcgt ctgctcgagg acgtcctcga gggtccggtc ctgctcgtcg
    45121 ctgccgactc cctgggagag cgactccgtg ttccgggtca ccgccaggag catctcgacg
    45181 gtcgcggggt cgaggcccgt ccgctccccg atctcctcgt gcgtcggctc gcgcccgagc
    45241 tcccgctgca gctcgtcccg ggcccggacg gcccggaact ggtgcgtcgc ctgcttgtgg
    45301 gggatccgga tcgtgccggc ctgctcggcg agcgcgtgcg agatcgcctg tctcacccac
    45361 cagaccgcgt aggtgatgaa cttgacgttt cgattcgcgt cgaaacggcg ggccgcctgg
    45421 atcagcccga tgttcccctc gttgatcagg tcgatgaagg ggacgttcgg ggagtggaag
    45481 cccttcgcgt ggtagacgac gaagcgaagg ttcgcgcgga cgagctcgtc gatcgcctcc
    45541 ttgcgccgct cctccggcac gagccggtcc tggatgacgc gcgccagctc cttctctcgc
    45601 tcggccgaga gaggggggta cttcgagatc tcctggagat acctccggag gagacccgag
    45661 tcttcgtcgg cccgcctttg gttcaaagct cgtacatcgt accccgccgg cctcgcggaa
    45721 gggaggggcc cgccgacgtc aggacgtgcg gcgggccagg ggcgcccgcg gccgaagggc
    45781 gagggcgttc tggggcgggt gcggccgctc ggcggcgcgc gaggcgtgct cggcggccac
    45841 cgaacggaac gcctccatga ggcgctcgac ctcgccctcc atcttcgaga gccgggcgcg
    45901 gaggtcgagc acgacctcca cgcccgccag gttcaccccg aggtcgcgcg tcagcgtcag
    45961 gatcatctcc agccgctcca cggtctcgcg gtcgtagagg cgcgtgttcc ccgaggagcg
    46021 gggggggcag atgagcccct cccgctcgta gagccggagc gtctggggat ggacccggaa
    46081 tcgctccgcg acgactccga tcaggacgta gtcgtcgcgg cgcgtccgcc cgcggcctga
    46141 accttctctc gaggcggcca cggcgtcact tcttctcgtc gacgacctcg gcgtcgatca
    46201 cgtcgtccgc gctcttcccg gagcccgcgg tcgacgagcc cgccggggcg cccggttcgg
    46261 gagccggggc gctcttgtag agggcctcgg cgaccttgta gctcgccttc tggacctcct
    46321 gcgccgactt ccggagcgcc tcggcgtcgt cggcctgcgt ctcgaggacc ttcttcgcgt
    46381 cggcgacggc cgcctcgatg gcggtcttgt cctccgccgg gaccttgtcg gcgttctcgg
    46441 cgaggagctt ctccgtcgcg tagacgagcg agtcgagctg gttcttcgcc tcgacggcct
    46501 cgcgccgctt cttgtcctcg ctcccgtgcg cctgggcctc cttcaccgcc ttctcgacct
    46561 cgtccttggc gaggcccgag gaggaggtga tcgtgatctt ctgctccttg cccgtgccgc
    46621 ggtccttcgc ggtgacgtgg aggatgccgt tcgcgtcgat gtcgaacgtc acctcgatct
    46681 gcggcatgcc tcgcggcgcg ggcgggatcc cgacgaggga gaagacgccg agcagcttgt
    46741 tgtcggccgc catctcgcgc tcgccctgga gcaccttgat ctccacggac gtctgcccgt
    46801 cggccgccgt cgagaagacc tcgctcttct tcacggggat cgtcgtgttg cgctcgatga
    46861 ggcgcgtcat gaccccgccg agcgtctcga cgccgaggga gaggggcgtg acgtcgagga
    46921 ggaggaggtc cttcacgtcg cccgcgagga cgccaccctg gagagcggcg ccgaccgcga
    46981 cgacctcgtc ggggttgacc cccttgtggg gctccttgcc gaagaagtcc ttgacgacct
    47041 gctggatctt cggcatccgg gtctggccgc cgacgaggac gacctcgtcg atctgcttcg
    47101 cctcgaggcc ggcgtccttg agcgcctggc ggcaggggcc gatggagcgc tggaggaggt
    47161 cgtcgaccat ctgctcgagc ttcgcgcgcg tcagcttcac gctcatgtgc ttcggacccg
    47221 acgcgtcggc tgtgatgaac ggaaggttga tctccgactc gatcgtcgac gagagctcga
    47281 tcttcgcctt ctcggcggcc tccttgaggc gctgcaccgc catcgggtcc ttgccgaggt
    47341 cgatcccctg gtccttccgg aactcggcga ggagccagtc gacgatccgc tggtcgatgt
    47401 tgtcgccgcc gaggtgcgtg tcgccgttcg tcgacttcac ctcgacgacc ccctcgccca
    47461 cctcgaggat cgagatgtcg aaggtgccgc cgccgaagtc gtagacggcg atcgtctcgt
    47521 tgtgcttctt gtcgaggccg taggcgaggg ccgcggccgt cggctcgttg acgagccgct
    47581 cgaccttcag gccggcgatc tggccggcgt ccttcgtcgc ctggcgctgg gcgtcgttga
    47641 agtacgccgg gacggtgatg accgcccggt cgaccttctc gccgaggtgg gcctccgcgg
    47701 cctccttcag cttcgtcagg atctgcgcgg agatctcggg cggcgacatc tccttgccgc
    47761 cgaccgtcac gcgggcgtcc ccgttggccg accggacgac ctcgtagggg accatcttca
    47821 tctcttcgtt gacctcgtcg aagcggcgcc ccatgaaccg cttgatcgag tagacggtgt
    47881 tctccgggtt cgtgacggcc tgccgcttgg cgacctgccc gacgaggcgc tcgcccgact
    47941 tcgtgacggc gacgacggac ggagtggtcc ggcccccctc ggagttcgcg atgacctccg
    48001 cggagcctcc ctccaggacg gccacgacgc tgttcgtcgt tccgaggtcg atgccgatga
    48061 tcttgctcat cctgattccc tccggacccc ggcgggtccg ttgcggaatc taggatgtta
    48121 gcgcgatatt gtcaagtcca cgacgacaaa tatcttcagc acgaccgaag aaatcatgca
    48181 aatcactgaa tgagtatgat ttccttgttg tcgcccgagt ggctctctgt cccggacatt
    48241 ccgccgctgg ccgcgtgcgc gaagaaaaga tcagtgtcgg tctttccgtc tgcgccgaat
    48301 cccgcgaggt agtacttccc ggtctcgccc tggaggatgt accgataggc gcgcccccag
    48361 gggtccagga tctcccccgg ccccagcagg cccgcgtcga cgatcgcgct cggcgactcc
    48421 gggaagaccc cctgcgtcag gtagtaggcg tcgatcgctt ccgagagcct ccgcagccgg
    48481 gagagcgaga tcgccttctg gaagccctcc accgccgcga cccggcgtcc cgggatcgtg
    48541 aacgcgttca gcgcgttccg gtgctggatc aggaacgccg ccccgaggag gacgacgagg
    48601 accgcggcga ggaccgccgg aaggagacgg ctcggccgga cgatcggcac ctcgacggac
    48661 gacatccccc tctcgtcgac gacgcccgtc cgccgcgcct cctcgacgag cccccggctg
    48721 aggaggtcga agaatgcctt cgacccctcg aactcggaaa ggaacgtccg gtcgacgatg
    48781 tccccgaccg tgcgcctccc gtcgacgagc tcgtagaccg cccactcggc gcgggagacg
    48841 cagatcgggc cgtcacgccg ctctctcgcg cgcgatccca ccggctcccc gatcgcctcg
    48901 acgccgtcga ggtcttccgc cgcctcgacc cgctgcccga ccgggacccg ctggaagacg
    48961 agctccggcg agcggacgac cttctggatg atcggccact cgtcgatcat ccgggcgccc
    49021 tccatcagga ggccctcgac ctggatcggg tggacgtact cccgatcgta gtcgatcttc
    49081 tcctgcggat cgaagacgta gtcgccgtcg gtccagcgga agagcccgta ggcgatcttc
    49141 cggacctgcg cgtcgaggcc ggcccgaagc tggtcggcgt cgcagaagcc gttcttcagg
    49201 aggatgaagc cgaggcgctg cagggtctgt ccctggatct ccagcgcctt cgtgagcgac
    49261 tcgggagaga ggctgcgcgt cttgacgagg agggtcccga gccgggtgtc gatccgccgc
    49321 gcctgcgact ccgccgagac gagcagccct ccgtcgaacc cgaggatgac cgtgtcggcg
    49381 ggaccgcgca gggtcaggag gccggtcttc ttctggagcg cgatgagctg gaggatgtcg
    49441 gccagggcga agtcgtgaag ggtcccttcg agcgccacgt cgtcacccct tccgcgccgc
    49501 gagctggccc gcgacccacg ccccggcggc gacgaggccc caggcgagcg tccccgccgc
    49561 gagccgctcg gcgcccggac gcggcgagag ggcgaggtga tcgcggaaca gggccgcgag
    49621 gagggcgaag aagaagaggg cgaggatcgc cgcggccttc gcgacccgcc cgtcgaggaa
    49681 cgcctccccg cccggcagga agaggttgag gaccctgcgg atccgctcct cggtcccctg
    49741 ccggcgcttc acctcctcga ccttctggag cttcgtctcg atcgagaccc cgtccttctt
    49801 caggtagacg tggacgcact gcgagcagag gagcgggctc tctccgggag gcttgcagag
    49861 ccggcagaag gtccgtccgc atttctggca ggcgacggcg tagccccgcc ccttccgtcg
    49921 gagcgagtcg agcaggaagg ccgccgccat cgcgaagggg atcgcgacgg cgagcggaac
    49981 ggcccacgtc ccgacgacgc tcccgacgtg gaagtgcccg aggactctcc ggctcctcgg
    50041 gtcgcgctga aggagcccga tcttcgcgcg cgcctcgtcg ggcgtgtagc cgagggagac
    50101 gaccttcacg agcgtcgggc tgtcgaggaa cttcgtgacg agcgcgttgt cgagggcccg
    50161 cgcctcggtc agcttctcct gcgcttccac cgtccgaaac gtctcgccgt agacgagcga
    50221 gaggttgtac cgcgcgcgca cgtcgcgcgg gtcgcgccgg agggcctcca ggaagtcctc
    50281 ctgggccgcg ccgaagtcgc cgtcgacgaa ccggatgtcg ccccggttga cgagcgcgcg
    50341 cacctcggcc gtcgagacct gcgcggcgac cgtgtactcg gcgacggccc ggtcgttctg
    50401 cccgagctgc tggtagaggc agccgaggag gaagcgaacg tcggcgtcct cggggaagag
    50461 cgtcttgacg gcctcgaggt cgtcgaggac ccgctggtcg tagcgcttct cctggagcgc
    50521 ctcggcgccc cggaggaccg gcgaggcgga gaccgacagg gagaccgccg cacggtcgag
    50581 cgccgggggg acgagaagga gcggcacgag gccagcgacg gccgcccacc gcacgctccg
    50641 atcggcatag ccgagggaga gggcgaagag ccagaggagg agccagccga cgtccagcgc
    50701 gaacagcagg gggagcagca aggcggccag cgccgcgacc ggctcggcgg acgggggaac
    50761 ccagcgcccg gccagctcgg tcaggtcgtg gaagaggcgg cggaccgcgc ccgccacgag
    50821 cagcacggcg aaagacagcc cgaccgcggc gagcgtcagg acgaggagga gcgatgcccg
    50881 gacgagaagg acccttctcc cctcgaggtc cacgacggag gcgcgcagag cccccacgag
    50941 ctcccgtggc acggcgaggt gctcggcctt cagcgcgagc cggacgcgcg cccagcgggc
    51001 ctccggaagc tcggggtcga gccgcacggc ggcgtcgagc gcccagcgcg ccttctccgg
    51061 gctcccctgc gtcgcctcgc ggcgcgcgag gagcgtcgcg gccagggaga ggtccgtgag
    51121 ccgcttcagg ccgatcttcc gggcgtgctt gacgatctcc tcgaccttct gggccgcaag
    51181 gagagggtct ccctcttcgt gcgcggcgag ccacgcgaac cactgcccgc gcacctcgcg
    51241 gcggaacgtc gactccgtcg cctcgcccgg cgcctggagg acctgggcgc tcgcgagaag
    51301 gccggacagg agcgggagga gaagcagcgc gagagccgcc cgcagcgccc tcgggcgtcg
    51361 agggccgagc cccgtctggc ggcgcatcgg ctcccgcagg gtcagccttt cggcctcggc
    51421 ggaggcggca tggcacctcc tccccgggcc tgggtcgcga ggcgggtgta gacgagctga
    51481 agctcgccga ggagcccttc gagcgcatcc gtctcctcga aggagagccg actccgggtc
    51541 ttctcccgga gggccaggag cgtgtcgatg atctcgcgcg aggcgttcag gtcgaccgcc
    51601 cgccgccccg tagccgggtc cgggacgccc tggaggtaga gcgatgccgt ctgcgcgagc
    51661 atgtcgagga gccggaggaa cgccgggctc gttggagtcg cttcctcggg tgcgggggga
    51721 gcggccgctt cgggtgcggc ggaaggagtc tccggcgccg gcggcgtcgc cgccagctct
    51781 tctttcacgt cgtcccgcag gtccccgtcg gcggtgaaca tcctgcggtc cacgaccttg
    51841 atcgtcttgg gtgcttctga cataaggcct cgttcgggcg cttcattcta ggggatgttt
    51901 ccaccggcgc ccgcccgagg aggtcccccg tgccgcccga gccgagagtc ccgcgtcccg
    51961 aagccgccgg cgaggccgcc gccgccctcg tgcgcctcat gtcgcgcctg cgcgccgcgt
    52021 gtccctggga ccgcaagcag acctgggcgg acctctccgg gtacctcctc gaggagtgtc
    52081 acgaggccct cgaggcgctc gcgcggggca gcctccgcga cctcgaaggc gagctcggcg
    52141 acctcctctt ccagatcgtc ttcctcgccg agctgggccg ggagtcggac gccttcgacc
    52201 tcgcctcggt cgccgagcgc atccacgcca agatggtctc ccggcatccc cacgtcttcg
    52261 gcgagagcga ggtgtcggac gccggggcgg tgaaggtcca gtgggagaag ctgaaggaga
    52321 aggagcgcgc cggccaggcg ggcgaggccg agccgccctc tccgttcgag ggcgtcccgc
    52381 gggccctccc cgcgctcctg aaggcggccc gcgtcacctc ccgcgcggcc gatctcgggt
    52441 tcgactggca gcgcgacgcc gacgtcctcg cgaagctcga cgaggaggtg gccgagttca
    52501 aggccgagct cctcgcggag ggcgccacgc gcgattcggt cgcgaacgag atcggggaca
    52561 tcctcttcac cgtcgtgaac gtcgcgcgga ggcacggcgt cgaccccgaa gccgccctcc
    52621 aggcgacgaa cgcgaagttc gtgaggcggt tcgaggaggt ctcccggcgc gttcgggcct
    52681 ccggtcgcga cgtgcgcgac acgcctctcg aggagctcga ccggacctgg gacgaggtga
    52741 agcggggcga ggcgccctga cgagaggcgg ggtcagcccg gaccgtgcgc gccggcgcgc
    52801 gccgactcgc gcgccgcctg caggacggtc gcgaagacga ggtccccctc gtgcgtggct
    52861 ccgccgaccc ggaccctcac cgggtgaggg acgggactcg cgacggtgcg ccacggacgg
    52921 aacaggagat cgagctggcc cactttcagc gcacaggcct cggcgtcggc gttgaagagg
    52981 agcaggagga actcgtcctc ctcccagcgg aagaggtggt ccgtctcccg cacgctgccc
    53041 tgaagggccc gggccgcctc cacgacggcg ccgagcgccg tctcctcgcc cgccttcgtc
    53101 agctcggcga agttcgcgag gacgaccgag aggacggaga ggcggatccc gtggcggcgc
    53161 gaccgctcga gctcccgcgc gatgacgtcg tcgaagtact cccgtacgta gaggcccgtc
    53221 acgacgtccc ggatccccgt gtcgccgccc atcgcgcggc gatcttatca gcgcgggaac
    53281 cgcctcagcg gtagcgaacc tcggcgggag acgtcggccg ctccgtcccg cgcgcctcga
    53341 gcgtcgtccg gtcccgcccc tcggccttgc tccggtagag ggcggcgtcc gcccggcgga
    53401 tcaggtcggc gacgccgcac ctctcgaagg cgccgtggtg gagcgcgacg cccgcggacg
    53461 cggtgaccgc gtcggggacg tgcgggaggc ggacctccgc gatcgccgcc cggacccgct
    53521 cgaccgcggc gacggccgcc tccgcatcga cgtcgacgag gagcaggacg agctcttcgc
    53581 ctccgtagcg gcccgcgagg tcgatcccgc ggacggcgcc ggcgacggcg cgccccgcgt
    53641 ccttcaggac gcggtccccg gccgggtggc cgtaccggtc gttgaagcgc ttgaagtggt
    53701 cgacgtcgag catcgcgagc gccagggagc tgccgttcct ctgggcgcgc tcgagctccc
    53761 ggcgagccct ctcgaggaac gtcgcgcggt tcgggagccc ggtgagcgcg tcggtcgtcg
    53821 ccttctcgag gaggcgccga ccctggtcgg ccaccgcgac gagcaggagc gccgtgaaga
    53881 gcaggaacgc cggcccgagg aggggtggga ggcgctcccc gggcaggagc acaccgttcg
    53941 cgacggcgac gtcgaagagc gcggagagga ggagaagcgc cgtggccgcg aagaggaggg
    54001 ccgcgtcggg ccggcgcagc cgcacgaggt cgagcagggc cgcgccgccg aacaggacga
    54061 gggcggcggc cgccgcggga gccgagagcg cccgggcggc cggaagggcg acgacgaccg
    54121 gcagcgcgag ggcggggagc gcgagcagag agatccgtcc cttccggagc gggaggtcga
    54181 ggaaccggac gaggaggagc cacgcgagcg ccgggaggag gaggccgagc gcggccgcgg
    54241 cccgggcggc tcccgcagag ccctctccga ggccgagcag cagataggac gcgagccccg
    54301 acgcgaggaa cgtgtagagg aggaggtccg tccggctccg gtcccgcgcc cagaagagga
    54361 gggcgaccag gccgagcgtc gcgatcgcga cggcggaggc tcccggcacg aggagctccg
    54421 cggggagctc gagccgggag gctcccgcga acggctccgc gggaccggcg ttcgccggcg
    54481 gcgcgaggag aagggaagga gacaacgcct tcgatgatag gcgcgctccg ggcgccgggg
    54541 accgcgagga ccgccggcga ttccgtcgac gtccgggata cgattccggc gtggcgttgt
    54601 tcgaaagccg tcccgcgggc gacgcccgcc accgcgaggc cgtggaggag tgccggaagc
    54661 ggctccgcgc cgaccccgcg gacggtgccg cggcgctccg gctcgccgac ctgctcgcca
    54721 cgccggacgg gcgggcggag gcgatcggga ccctcaaccg cgtcggagcg cgcctccgga
    54781 aggccggccg catcgtcgag gcgatcgccg tcttcaagaa ggtcgaagcg ctcgaccctc
    54841 gggccgaagt cacttcgagc tacctctcga tcctcgagct gaagaagctg caggaggcgg
    54901 aagggcggag ccggtcctcc gcggcctccg ccccggcaga gtcgcccggc gacccggctg
    54961 cgaccgaccg gaggaggaag tccgagcggg tccactccct gcggaaggag gtccccctcc
    55021 tccgcgacat ccccgcgttc ctcttcgagc tcgtcctcga gaagatccga ctctcctccc
    55081 tcgacgcggg cgccatcctc tactccgagg gggatcccgg cgggaccctc ttcttcgtgg
    55141 cgacgggaga ggtcgagacg tccgcccgcg gcgactcggg cgcgccggtc cctctcgagc
    55201 tccatcggac cggcgacgtc ctcggcgtcg tctcgttcct gagcggcgtc ccgcaccccg
    55261 aatccgccgt ggcccgcacg cacgccgacc tcctcggcct cgaccgcgcc gcgctcgctc
    55321 ctctggtgaa gaagcaccgc gagatcgccg aggccctctc gcgcctcttc gccgagcggg
    55381 tcctcgacgg ggtcctcgcc cgctcccgcc tcttcggcct cctgccgcgg cgcgaccggg
    55441 aggccgtcgc ccgccgcatg cgcccgctga cggtgaaggc gggagcggtc gtccttcgcg
    55501 agggggagcc cggcggcgac gccttcctcg tccggagcgg cgcgctccgc gtcacgacgc
    55561 gccacgccgg gcgcgagctc gccctcgcgc tcctgacgcc ccacgagctc ttcggagacg
    55621 tcgcgagcct ccgccacggg ccgcgcaccg ccaccgtgac ggcggtgacg gagtgcgagc
    55681 tcctgcgcct cgcggcgtcc gacctcctcg ccgtcctcgc ggctcacccg gagctcgccc
    55741 ccgcgctcga ggagatccag ctcgaccgct tcacgaggaa cgccgagaag ctggcgaagg
    55801 ggacctgaga gcgacgggcg cgggcggatc ggcgatcatg cgccgccgtg cccgccccct
    55861 tccccttcgc cgaccggccg cgcctgttcg gcgtcgccgt ctcccactac caggtcgagg
    55921 gggacgaccc gtgtgactgg accgactggg aggccgccgg acggacccgc ggcggcgcgt
    55981 gcggccgggc ggccggctcc tgggaacgct acgaggacga cgcggcggcg gcggtcgcgc
    56041 tcggggcgaa tgcgttccgc ttctccgtct cctggagccg cgtcgagccg cgctacggga
    56101 cgttcgacga agcggcgctc gagcgctacg cgcggctcgt cgcgcggctc gccgccctcg
    56161 gcctcgagcc ggtcgtcacg ctcctccact acacccaccc gcgctggttc cacacggaga
    56221 cgccctggac ctctcccgcc tccgtcgacg ccttcgcgcg cttcgccggc cgcgtcgcac
    56281 gcgcgctcgg tccggccgcc cgctgctgga cggtcctgaa cgagccgttc gtcctcgttc
    56341 tcggcggcta cctcgacggc caggtacctc cgggcctcgc ctccggccgg gacgcggccc
    56401 gggccttcga caacctcctc gccgcgcacg aggccgccgc cgcggcgatc cgggaggcct
    56461 ctcccggcgc ggcgatcggc gtcgcgcaca acatgatggg cttcgctccc gcgaggccgc
    56521 ggaacccctt cgaccggctc ctcgccgcgg ccgccgggcg gttctacaac cgacccctcc
    56581 tcgacgcctt cggcgaggga cgctggagcg cgttcctccc gccgttcacg cgcctcgcgg
    56641 gacgacgccc ctctctcccc gcctccctcg acttcttcgg cgtgaacttc tactcccgcc
    56701 tccacctccg tttcggggcg ggcggccgcc ggatcggatc cttcgactac cgcgacgcca
    56761 cggggcgcgg cctcaccgac aacggctggg agatcgtccc ggaggcgttc ctcccgctcc
    56821 tgcgggaagc ggcctcgatc ggccggccgc tcctcgtcac ggagaacggc ctcgccgacg
    56881 ccgccgacgc ccgccgcgcg gcgttcctcg aggagcacgt cgcagtcgtg gcgcgggccg
    56941 aggcggaggg ggtcccggtc cacggctacc tccactggtc cctcgtcgac aactacgagt
    57001 ggctcgacgg cttcgagccc cgcttcggcc ttctcgcgct cgaccgcgag acgctcgcac
    57061 ggcgcgagcg accctcggcg gaaacgttcc gccgcctcgc ggcggagtat ctcggccggc
    57121 ccgcggcccc gcgccgcggc tgatctttac ccggtcctca tcgtctcctc accccgtcct
    57181 cccgcgccgc gccgatcttc ggggcgcgag aaaggagggt gcgggatgct cctcggagac
    57241 ttccacattc acacgacgtg gtcggacggc cggcatgccc tccccgaggt cgtcgacctc
    57301 ttcggtcgct ccgggcacga cgtcatcgcg atcaccgacc acgtcgtgaa cgccgactcg
    57361 ctcctcggga gggcggccca cggcttccgt ctctccgtct cgcgcgcgtc gtggcccgcc
    57421 taccgcgccg agatcgagcg ggaaagccgg cgcgcctggg acacctaccg gatgctcgtc
    57481 ctgccgggcg ccgagctgac gcggaacggc ctcaccgggc ggagctcggc ccatgcgctc
    57541 gccctcggcc ttgacgagtt cgtctccgcc gacggacccg tcgaggaggc gctcgcgagg
    57601 gcccggg
//
LOCUS       DAOONL010000013        57568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_364_length_57568_cov_17.549319, whole genome shotgun sequence.
ACCESSION   DAOONL010000013 DAOONL010000000
VERSION     DAOONL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 57568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57568
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_57568_cov_17.549319"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLP50_03700"
     CDS             complement(<1..265)
                     /locus_tag="PLP50_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50685.1"
                     /translation="MRRGGEAARAGSRPSPGRGALLPRLGWLLAALLFLVFVAGLVRR
                     GRIERAGEQVIRMLFVPSVEQGTLARRGNELAEFVRKDTGLVIR"
     gene            367..792
                     /locus_tag="PLP50_03705"
     CDS             367..792
                     /locus_tag="PLP50_03705"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPA50686.1"
                     /translation="MDTPLGRTLAVTKALANPARLRILAILAGGECCVCQLTAVLSLA
                     PSTVSAHLSELRRAGFLLVRKAGKIVFYRLDERAALSVWRRAALAGLHDDARVAEDRA
                     LLERVKTVDVETFCGAGPRWRLLPAFRPSKGAAAPRRRR"
     gene            complement(821..1663)
                     /locus_tag="PLP50_03710"
     CDS             complement(821..1663)
                     /locus_tag="PLP50_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPA50687.1"
                     /translation="MDAPLAPRAGRLSIAGAALLWSTAGLAIKLVPLDAVGVVFWRSS
                     ITFLFLAAVYRPGIERWKKASLATALSYALMILCFVSANKLTTAANAIFLQYTGPFYV
                     LALGPWLLKERFRKEDAVAIAVALGGMSLFFVGRLDGGDLAGNLLAVVSGLFFALTVV
                     LLRRDQSRDAMASVFLGNVLAALLALPFAWGNLSLDGRGLGVIVFLGVVQMGLAYILF
                     VRGLSVVPAAEASLIGMLEPTFNPLWAFLGLGERPSGWALLGGGIVLAAVAGRTLWGV
                     RSAR"
     gene            complement(1664..3985)
                     /locus_tag="PLP50_03715"
     CDS             complement(1664..3985)
                     /locus_tag="PLP50_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPA50688.1"
                     /translation="MTHPSRPPNRLANEPSPYLRQHAHNPVDWYPWGEEAFEKARREG
                     RPLFLSIGYSACHWCHVMEHESFEDEGVAALLNERFVSVKVDREERPDVDDVYMSAVQ
                     TMVGRGGWPLSAFLFPDGRPFFGGTYFPREDRRGQAGFRTILEKLSEAWRERREELSK
                     GADEIVAEVERAGRITDRAGREPLTARTGSVLTETLKRTFDARHGGFGGAPKFPPHLA
                     LEWLLLKGVEGDSIARSMALKTLDAMALGGIHDHLAGGFHRYSTDERWLLPHFEKMLT
                     DNAQLLALYARAFVLTGRDLYWRTARGIGDWLLSEMRGEEGGFFAATDADSEGEEGRY
                     FVFTEEEVRAVAGDDAALFASAYRVRPEGNFHDESTGRATGRNVLHLAEEPSSEEERR
                     LAPIRARLLERRRLRVPPGTDDKRIAGWNALAVSGLAVASSILGEPRYLDAARASAEF
                     LLTRMRDAGGRLYRTWKDGEARIPAFLEDEAFLALALLDLAEAEPDPSGKARWAAEAR
                     RTVAGLRARFGRPGVPGLAFTGEGHERLFARTRDLFDKAIPSAPAAAARAMARLARRD
                     GDAALAAAALAEVEEVSGLMERVPHGTESWHLAWEELRTSGLLRPAGAAPEAEAGGRD
                     TADDEESGRSKPVPAETPASSGPVRVAASLSSDRASPGEMLEVRIAIDVAEGWALTGE
                     SPLAVDAWGGPGLHCVAVELPPRGTVAHADGSSEPGYEGRIGVTLVFQVREGAAEGEH
                     PVAISLRYRACGDGTCLPERALALTVPVLVLGR"
     gene            complement(4020..6806)
                     /locus_tag="PLP50_03720"
     CDS             complement(4020..6806)
                     /locus_tag="PLP50_03720"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPA50689.1"
                     /translation="MESAPRSAGAWQRSLAEVLADVGSEAGTGLASAEAAARLGRHGS
                     NELTERAGRSRLEIVREQLLNVMTVILVVAAALSVVLGDWVEAVVILAIVVLNAALGY
                     SQEYRAERSMAALKRMSVPTVRARRDGRLQEISSRDLVPGDVVLLETGNVVPADGRVL
                     SSVNLRAQEAALTGESEAVEKDAGVVLEPERPLAERRNMVFAGTVVTWGHGEVVVTAT
                     GMATELGKIASLIQSVVEEKTPLQQRLDRMGKGLAIGAGVLVAVVFLLGIRSAGTREE
                     VFEVLLTAVSLAVAAIPEAMTAVVTIALSLGAQRMLRRRALIRKLPAVETLGSVGVIC
                     SDKTGTLTQNRMTVTALDVADHRIDLVQREGTEHFSLRRVGAGEAEPELAPTLDLLLA
                     SGALCNDASLVKDEGAGGAFHAVGDPTEGSLVLAAAEYGARKDELDRALPRVAELQFD
                     SVRKRMTTVHQLPAGDAELPPSLRPLWARRVLPEETPRYLAVTKGAIDGLLGKTRSVW
                     SGESAVPLDDEARRRVMAAHDDLAAKGMRVLGLAVRPLDGPPSAAELASLEEELLLVG
                     LFGMLDPARPEVADAVSRCRAAGIRPIMITGDHPLTARHIAKQVGITEGDEFRTGADI
                     DRLSDAQLARAVGEVPVFARVSPEHKLRLVASLQAQGQVVAMTGDGVNDAPALRKADI
                     GVAMGITGTDVSRDAAEMVLLDDNFATIVAAVEEGRKIYDNIRKFIKYLLSCNASEIA
                     VMLLAPLFGMPLPLLPLQILWMNLVTDGLPALALGVEPAEADIMKRPPASSEESIFGR
                     GLVPFVLVMGVVMSLIALGIGLWAYRSGDPAWQTLLFTTLIFSQVILALEVRSEKASL
                     LTLGLLTNPLMVAAFLSTVGLQVAVVYVPFLQRVFSTAPLGARDLGIAFGSGLAVLLV
                     VEAWKAVRRRRGGP"
     gene            6890..7429
                     /locus_tag="PLP50_03725"
     CDS             6890..7429
                     /locus_tag="PLP50_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA50690.1"
                     /translation="MTANPQRPHTRPTSTVPALRHALPLLLCALLTLAVARPSPAAAP
                     EKALVSWDDAKRISIDELARLLKDPTAAKPEMFHVGFRVLFVQAHIPGSRFAGPGASP
                     AGLETLRKAVAAVPKAKTVVLYCGCCPWEKCPNMEQPWRLLVAAGFTDVRVLYIPTNF
                     GRDWVGRGHPSASGSGDRG"
     gene            7422..7943
                     /locus_tag="PLP50_03730"
     CDS             7422..7943
                     /locus_tag="PLP50_03730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA50691.1"
                     /translation="MAERLRRLRALGGLVACAALLSLAVRAEDPPAPKIGDPAPALGL
                     VDLDGKKLDLSSLRGNVVVVDFWATWCAPCRSQAPRFEALQEKHRGRGLVVVGVSLDE
                     SAEPVLKFRKELGLTYRIVLGDETVAERWGGILGLPVAFVVDRQGRLRARHEGEHDLA
                     ALEADVVRLLGEK"
     gene            complement(7949..9235)
                     /locus_tag="PLP50_03735"
     CDS             complement(7949..9235)
                     /locus_tag="PLP50_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPA50692.1"
                     /translation="MTGVLVVLALVVVNGLFAGAEIAVLTLRRTRLDELVEEERTGAK
                     ALAALRADPEGFLATVQIGITLVGAAAAAFGGATLSSPLAAALEAAGAPRELADDVAL
                     AVVVVVVSFLSLVVGELVPKSLAMRFAERYALAIARPLALLARLARPAVRFLTACSNG
                     VLRLFGDRTSFVEARLSRDELRTLVEETATTGGLDPRAGEIAARALDLPGVKVGAVMT
                     PRPSVVAIDATATREEIVSAVSRSRFARFPVHDGGLDGVTGYVLGREVLARILEGATL
                     PLQGIVREAPFFPETVEALGVLRALQERHVPIGFVVDEQGGFTGLVTLEDLLEELVGE
                     IFHEKEADAVPYREDGPGAWVVEGHAGVRDVNRLLDLELPEDPGWATIAGLILHRLER
                     IPPAGTAFVDEESGLTIEVVESTPRRIARVRLSRPA"
     gene            9333..10475
                     /gene="chrA"
                     /locus_tag="PLP50_03740"
     CDS             9333..10475
                     /gene="chrA"
                     /locus_tag="PLP50_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HPA50693.1"
                     /translation="MERTTLGEIAAVFLRLGTTAFGGPAAHVAMMRDEVVRRRGWLTD
                     AELLDLLSAANLIPGPNSTELAIHVGWARRRWAGLAVAGLSFILPAALITAALAWAYV
                     RFGALPEADRLLWGVKPVILAVVVQAIVGLAPHAARTVPLRLLGIVAAVAAALGAHEL
                     VVLLGAGLLVAVVRAVRSRGAGGNAALPALGPLFPVAAGAAGLSAVGAVTLPAIFWLF
                     CRTGAVLFGSGYVLVAFLRTDLVERLGWLTEAQLLDAVAAGQVTPGPVFTTATFVGWL
                     LAGPAGAAAATAGIFLPAFVFVALSAPFVPRLRASRTAGAFLDGVNVASVALMAVVTA
                     RLAPAALVDLRAALLGLLAAVLLVRYRTGSVVLVLGGAAAGLIIGP"
     gene            10472..10687
                     /locus_tag="PLP50_03745"
     CDS             10472..10687
                     /locus_tag="PLP50_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50694.1"
                     /translation="MTSIGDAFRRKTAEADSLLTPAERVARAFSLGNDDAEAFRRSRG
                     LTRAAAEAELAAKRRSGRIPSRVAGTG"
     gene            10684..11229
                     /locus_tag="PLP50_03750"
     CDS             10684..11229
                     /locus_tag="PLP50_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50695.1"
                     /translation="MSLLAVVAKHLSLRRTPFATVGGVALAIRGVARSTFDVDLLTLD
                     ASVLADAYWSLVTRDGAQVEIRRGDGDDPLRGLVRLVSRDERPVDVFVGRGAWLDGVV
                     ARAEPFDLLDTRVPVVRAADLVLLKLHAGGTQDLWDVTQLLATDDGTLADEVVARLAE
                     VPRGAAILWRRLQEAPHRGQA"
     gene            complement(11239..12330)
                     /locus_tag="PLP50_03755"
     CDS             complement(11239..12330)
                     /locus_tag="PLP50_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HPA50696.1"
                     /translation="MYAGFILLVLALLALDLGVFHRKAHVVTVREALGWSAFWIALGL
                     AFSGFIYLGYENHWLGLGLTPDLMTTRPVPVEGLGTVYNDGASALVKYVTGYLVEKSL
                     AVDNIFVISMIFTFFAVPALYQHRVLFWGILGALILRGMMIAAGARMIQEFSWIIYVF
                     GGFLILTGIKMLLLKEGETDPNRNLVVRATRKLFPVTERFHGEHFFVRAGTDASHEAE
                     TPGAAVVADRVVDAAKAGTLLATPLLLALVMVEFTDLIFAVDSIPAIFAITADPFLVF
                     TSNVFAILGLRSLYFALAGMIDSFRYLKVALAVVLMVVGVKMMTHTWLKAMLGENFNL
                     YLLAVVLSILAAGVVASLWKGRSEARSAA"
     gene            12622..13686
                     /gene="rsgA"
                     /locus_tag="PLP50_03760"
     CDS             12622..13686
                     /gene="rsgA"
                     /locus_tag="PLP50_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPA50697.1"
                     /translation="MPLTRFGWDRDFEAQFENHAREGLVPGRVLTASREIYRLATAAD
                     EIPAELSGRLRYAAESSADLPAVGDWVAARIYPGDGLAVVDSVLPRRSALVRRSSGRR
                     DEAQLLAANVDLLLVAAPLGGELRERRIERFLALAREAKVRPAVLLTKADLAYDSAAD
                     LAAARAVAGDAPVVVVSARTGAGLDALDPLLPPHRTAALLGPSGAGKSTLVNALLGER
                     RQSTREVRESDHRGRHATTSRELFALASGALLVDTPGLRELALWDGAEAIAETYDEIA
                     ARAPACRFRDCRHENEPGCAVRAAADEGVLDPDRLAAFEKLRREDESLAERRALGPAR
                     AERERWRRMMGGAKGPVKLR"
     gene            complement(13696..15417)
                     /locus_tag="PLP50_03765"
     CDS             complement(13696..15417)
                     /locus_tag="PLP50_03765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA50698.1"
                     /translation="MPSARVRLLPETLLAILVALLVLAEPFRVPLVDPDEGRYAEIPR
                     EMLASGDWVLPHQNGLVYIEKPPLAYWLVAGSYELFGESEAAARLPGKLATIGTMLAL
                     FLFARRRAGERAGALAALFFGGSLLGFGLARVVLIDPILTAAQTAAVLSFAALGEGGA
                     SLRRERTLALGFAASTAAAVLLKGLVGVLLPGGAVLLWTAATGRTRPLRALLSPWTVL
                     TFLVLAVPWHVAAALREPDFLRFYFVREHFERFLLPNHHRPGAPWYFLAVVAGGFLPF
                     TPLLPRLRTVWPGRRRAGWFARPLESFLILWVLLVVAFFSMSKSKLIPYVHPVFPAIA
                     LLLALAVARDEEGLALRPGERWSIAGLLGVLAAAAAWAGAFEDLAVPSLAGPLLALSA
                     TLLAGFLLVGIVPTLVPERGLLLVASAWLLFLGGAHLVLPAWARWDGSWPIVTALRQT
                     LRPGDLLVQRGDYVESLPFYVRRLTPLSRIGTESELQFGRSHDRTGLFQTDEEFTRLW
                     NGPGRVLALLHHEVLGEWAAPGNRRRPYSILATAHRDRWVLVSNEAGKLLARAAIGSE
                     PAASAIR"
     gene            complement(15485..16795)
                     /locus_tag="PLP50_03770"
     CDS             complement(15485..16795)
                     /locus_tag="PLP50_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA50699.1"
                     /translation="MIVRSFARRTVAATLLTAAVSSLVFAVASVLALRLLWDRHDAVE
                     LSRTADAASRIFVLEQTEGEHDAQESAREALAQALEPADWGRFHRGRLLLAERGTPPA
                     REEDGRPPSGWIETRAALAGGIVLEVARRPEEGLPLRRLFWAALLVAAIPSSLVALAV
                     GRRAGRAAARPIEDLARRLGVIQHPRDYVPAPLDALPKEAAALEGSFASVLGRLEAAL
                     EREASFVRNAAHELRTPLTRIRLRAERAAAGASGACRLELQAVTNEVDRLTRLVDALL
                     VLARDESSGLDLKETANLADVVRASAESGERTVAVDAPDEALVRGDEELLRLAVDNLL
                     DNARKYAPADRVPRAVLTAAADGVTLSVETPGVSLPEEEREQVFERFYRGASARETAP
                     GHGLGLSLARHVARVHGGDVVAAEPGADRTVFELRLPGWKGTGS"
     gene            complement(16792..17490)
                     /locus_tag="PLP50_03775"
     CDS             complement(16792..17490)
                     /locus_tag="PLP50_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA50700.1"
                     /translation="MSDHAPRVLVVEDDPETREFVARALGEDGLDVKTASRAETARKL
                     VREGEFDLVILDVGLPGPSGLDLCAELRREESAVPILMLTARTDVASRVEGLEAGADD
                     YLGKPFALAELRARVRALLRRGARPGPSGSVLEHGGVRVDLRRRRAFLREEELPLTRR
                     ELDLLARLVRAGGVVVAREALLEDVWGADTPEAAASLEVIVSRLRRKLRQGGGGSLLR
                     NVRGVGYALDTEGA"
     gene            complement(17493..20612)
                     /locus_tag="PLP50_03780"
     CDS             complement(17493..20612)
                     /locus_tag="PLP50_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPA50701.1"
                     /translation="MPSPLYDSAESPEMKSFARVAPLLLATPLLAAPPATKTEEARRF
                     HPPAHGMPAAPRLAGFERRLAMESASPFAGLTFRNVGPELQGGRIVDLDAPRSRPDTL
                     SVAFATGGLWRTDDKGGTWTPLFDAESSIGLGAIAVGDAQGDVLWAGTGEANSSRTTY
                     AGTGVFKSTDGGKTWKNTGLHDSHHVGKILVDPASPDDVLVASMGPLYTDGGERGLYR
                     TTDGGATWTKVLGGPGRTGAIDVVRDPKDPKLLFAALWERDRKPWSFLESGPGSGIFR
                     SRDGGATWTRLEGGLPQGDVVGRIGLAVTPARPGAVWAVVDNQSRRPGERDEETPPGE
                     LTPRRLKALTAEQLAKVETAVVERFLRANGFPKELKARVLQRDVASGKVKLADVVAYV
                     DDADRQMEEVETVGVEVWRSDDAGETWRRTHDASLEKVAYTYGYYFGKIWVAPDDAEK
                     IYVAGVPLAGSSDGGRSWNGLDRHGVHVDHHALWFDPRDPRHVVLGNDGGLNLSWNGG
                     RSWTKVANLPVGQVTTVAVDSAEPYNVLVGMQDNGVWRGPSTYAAGKTPPDAWKPVIG
                     GDGSWIEVDRKDPDLVYTASQFGNAVRVNLKTGERTRFRPRHALGEPALRYNWVTPFL
                     LSPHSPNILWFGTQKLFRSFDRGETWAAVSPDLTSTREPGDVPFGTITTISESPKTFG
                     VLFVGTDEGKVWGTRDGGATWKDLSKGLAKERWVTRVVASVHDEGTVWVSQSGYRNDD
                     FAPYLWKSTDFGATWTSIAAGLPAEPVNTVREDPKAKHLLYAGTDLGAYVSLDRGATW
                     YALTGGLPHVPVHDLAVHPKEGDVVLGTHGRSAFVAEAAPLRELTPEVRAKPLHAFKV
                     KPATWERSRGYGENELFAWRRVPTTRTVSWWASPAAAGTATITVKDGKGRVWKEWTAP
                     SVAGFNAVAYDLSADRTRADANEEALRKEAAEAAAKAKEKTTATKESPDPVAAPETED
                     DEDDADAGPRATPPEVAALLADPLRATRDRYLPPGRYVVEVSVSGARAETRLLVKPPK
                     GDGAGRGGR"
     gene            20647..21612
                     /locus_tag="PLP50_03785"
     CDS             20647..21612
                     /locus_tag="PLP50_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPA50702.1"
                     /translation="MKLVTFVRPGADPAGAPGIALPGAGVLDLGRASETFRGLSMLDL
                     VRRHEELLPEARPLAAGEASLPAGTLVPEDEVRLLAPLPRPVSVRDGYAFRQHVETAR
                     RNRGLPMIPEFDEFPVFYFTNALAVIGPGELRVGRRRLEKLDFELECFVVLGKRVTNP
                     TLEEADDAIFGYGVMNDLSARTLQMEEMKLNLGPAKGKDFATAIGPWLVTKDELADRL
                     RPSPKGNVLDARMTAHVNGVQVSEGNVAQMDWTFAQIVQRAADGVTLEPGEVIGSGTV
                     GTGCFLELNGSGITKDQWLRPGDVVSLAIDGLGTLTHTIVEDPEK"
     gene            21609..22121
                     /locus_tag="PLP50_03790"
     CDS             21609..22121
                     /locus_tag="PLP50_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA50703.1"
                     /translation="MKDDLFALYDYNLWANRLLARSLAPLSHEDYVREMGGGWPSLRA
                     TLVHLAGATRAWAERFDGRDATSLPAVEELPTLADALRTLDEADARIAAFLATLGPER
                     LAAPLVWKNLKGEPKAAPLWAVLRHVVNHASYHRGQAAAMLRRLGGNPAPTDLVRWAI
                     ERHEAGAFEA"
     gene            22127..22825
                     /locus_tag="PLP50_03795"
     CDS             22127..22825
                     /locus_tag="PLP50_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPA50704.1"
                     /translation="MIETSFERLVRRAMILGIGNLATPVYFRWLNAVKAEGAELLKEL
                     PRKNVVFLSNHQTYFTEAIAFYDLLYIVHGLPYEDPHLRFSAATETMKKNLLTKVLTY
                     AGGVTFKRSFREGGREVNRPVDLEGVSKVEEAIATGWLLHFPTGTTQKNAPIRPGMAQ
                     LLHRTKPVAVPMRVDGFRELLLHKQFPGRTGKKATIRLFPPMDLTEFYGQPFDKERGR
                     KVIEELGKIILDPA"
     gene            complement(22806..23087)
                     /locus_tag="PLP50_03800"
     CDS             complement(22806..23087)
                     /locus_tag="PLP50_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50705.1"
                     /translation="MPVRKFRSIEEMKAARGYERTGPRLPRVIEGLWDFGRKTAKLRF
                     PPGVYRHRDLASLNAQSAAWADENFRAFQARRAAERSAAGEPWPTPGRG"
     gene            complement(23087..23308)
                     /locus_tag="PLP50_03805"
     CDS             complement(23087..23308)
                     /locus_tag="PLP50_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50706.1"
                     /translation="MNSPEGDFHVDILTRLGEAFRFEDLEPDETTLDGVKVRLVSPRT
                     LFRMKKDTVRPKDRLDADGLRAKYGFEEP"
     gene            complement(23314..23703)
                     /locus_tag="PLP50_03810"
     CDS             complement(23314..23703)
                     /locus_tag="PLP50_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50707.1"
                     /translation="MEIKGAGGTPGGVGTFLGGLAMMVVGGYLLLQQVQVHSGFWDWF
                     GHNTFGLTLVPLLVGIGFLFFDGKSVVGWLLTGAGALIIVAGIVANLQIWFRPTSLYN
                     TLIMLGLLAGGVGLVARSLKAKPEAKG"
     gene            23791..25089
                     /locus_tag="PLP50_03815"
     CDS             23791..25089
                     /locus_tag="PLP50_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50708.1"
                     /translation="MKRCSVGLMGTTFSVLVLAASPAEAQNLVQNGGFDRTISSWSSS
                     TSEISWSSDDWNRQPGSGSLSLRHTGTTANGYASVGQCLPSQPGKGYSFVLRARIVEA
                     TGGARGGVEAGVHFFSGTDCRGWLAGSDNRWVELSSSWTGVAYPFLIFPSAVSVAPAG
                     TRSVLVNVNAVKLTAAGALTFRIDDAYFAEAKPTVLTIPASASARGENDTFFQTDLWL
                     GVASSRADVPVAVLGEHRCFPGQTCTGRMHYWTDVLPRSFTRTVDTVGTLFKDPSTSG
                     AIELTYDASISTIAAMSRTYSPSLPAPTTGTAIPALPASEARTRSLLIGLSASGGDRS
                     SGFRTNVGAYNPGNASAEVTVRLYDAAGRTIGSPIERTVGPREPMQINEVFGEAGVGH
                     LLTNEAHAVVTSTRPVFAFATVIDNRSADSIYVPGAPDAP"
     gene            complement(25114..25965)
                     /gene="ispH"
                     /locus_tag="PLP50_03820"
     CDS             complement(25114..25965)
                     /gene="ispH"
                     /locus_tag="PLP50_03820"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPA50709.1"
                     /translation="MEVVLAEKYGFCAGVRVADKLVRIAVGKGQSGAILGQVVHNESV
                     EKEMASLGFPTVHRLEEAHAHSGRIVFSAHGVAPSVRDEAAALGLSAIDTTCKFVTDI
                     HREIGRSLDDGCFIAVLGQQGHREVIGYTKDLDPSVYGVFYTLEEVVAFPWEEHPKVK
                     VVFQTTLNAEGFAEHVAEMRRRVPDLRVADTICYATKENQDALRALCADPSIDAIVVI
                     GGRHSKNTKELVKIAEGAKRTVFVGTAADLRPEDFEGARKVGVTAGASTPDYDVEAVV
                     EHLRAMG"
     gene            complement(25968..28304)
                     /locus_tag="PLP50_03825"
     CDS             complement(25968..28304)
                     /locus_tag="PLP50_03825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HPA50710.1"
                     /translation="MTRKRSDGAGQPALAFDAEIALPEEARRRYLNYALSVITARALP
                     DVRDGLKPVQRRILFAMYQNLHLTPDAKFKKSASVVGDVIGKYHPHGDVAIYDAMVRM
                     AQPFSLRAPLVEGHGNFGSLDGDSAAAYRYTEARLRPLAVELLAEIRKRTVDWKPNFD
                     GVHFEPVVLPARFPNLLVNGATGIAVGMATSIPPHNPCEVLDAAIAAIDDPAADPLRF
                     IKGPDFPTGGQLLSTKKELREVYESGQGTLKLRAEYEVEERGRGAQAIVVTSVPYGVA
                     KASLVEKIAEVIIARKLPALVDVRDESTDDVRIVLELKRDADAGLVMSYLYKHTPLQT
                     TVPVNLTALVPTENPELCEPARLPLAAILRHFLDFRFATVKRRFEYELEELKKRIHLL
                     EGFEKIFDELDEAIRIIRRSDGKADAAAKLMKRFGLDEDQADAILETKLYKLAKLEIE
                     AIRKELAEKRAEAARIEAILRSPARLWGVVQRELVELRGVLSGEKRRTRVSAAVEEPE
                     YDAEAFIPDEDAFAVVTVDGWVKRLGQLKDPKGTRLREGDDVLAVLQGGTRELVVLFS
                     SRGSAYVLKMNDVPPSTGYGEPVQKLFKFADGEKVVAALSLDPRVTPPEDVVLLAATR
                     DGMVLRFSAEPLREATTRAGRRFAKPGDGDEVLLVGVCEPGAIVALASEKGRAILFDS
                     DEVPVLSGPGKGVYGIRLADDDRVVGAALFGRDEKRSAVLTLENSKGTPYTVTRRYDV
                     VGRGGKGFEMIKRDRFVKVVPPEIVLLDLPDRPTNGGK"
     gene            complement(28391..29884)
                     /locus_tag="PLP50_03830"
     CDS             complement(28391..29884)
                     /locus_tag="PLP50_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPA50711.1"
                     /translation="MTTDAVAAPPVDSTESADRHAFEAALEASFPASKRPQEGEAVSG
                     VIAGITPDVVLVSIGAKSEALMDLHELEGEKVGDRIDAVVIKAGPDIRLSRKLAVARR
                     AKAELRAAWETGIPVLGKVLSRNKGGFEVAIGGTGGPRAFCPVSQIDVGRHDEPGLLQ
                     FVGQSFDFRVIEYAEDGRRVVVSRAAILREALEKSLGEIRERIVPGAVLTGQVRSITD
                     FGAFVDLGGVDGLVHVTEISRRRVAHAKDVLTIGQEVQVKVTKVENEGKRISLSMKEL
                     EKDPWEGLAQRIPPGTAFTKPVARHAEFGLFIEIEPGIDGLVHVSQLPPGIDLKDPSV
                     AVGQEVSGWVREIDEEHRRLSLSLREASATDPWDGLAEKMPEGSVVAGQVENVAPFGV
                     FVRLEIGLTGLIPNSETGLPHGTPASKTFAPGQKVEVKVIGLDAQRRRISLSATGARE
                     EAERGEIRKYREDSAKRERSEPGRPTNFGASLMAALSSPNKPAKSAR"
     gene            30201..32198
                     /locus_tag="PLP50_03835"
     CDS             30201..32198
                     /locus_tag="PLP50_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPA50712.1"
                     /translation="MHLGRVLDDKAPNYLKQGLGWSYHAPCAGHDGIQVALGTVFRPT
                     FDYLFPYYRDLTTCLAAGVTPEQILLNGMSKATDVSSGGRHMSNHFARHDLYVWNVSS
                     CVSNHAQHAAGLGRAIRSYGLSSLVYCSMGESSTSEGYFYEAVNGSSLGKLPVIFVIQ
                     DNGYGISVPKRQQTANHYASDNFASFPNLKVVHCDGTDVLDSVRALREAAAFAQSGAG
                     CVLVHADCVRIGSHSNSDRHELYRDDEELAAARERDPLPRFRRYLLESGGFSEEELRA
                     LEDENARTYEAAADRARSAPDPDPRSIHDFVLPEPWVPDGDGLAGVARTPPASADGRA
                     NRESTLIHAINETMKDLFRENSDTFIWGQDVASKEKGGIFNVTKGMEKEFGSHRVFNA
                     PIAEDYILGMADGFSRICDGVRVIVEGAEFADYFWPAAEQMVEMSHEYWRTNGQFVPN
                     VTVRLASGGYIGGGLYHSQNVEGWLTTLPGIRIVVPAFADDAAGLLRTAVRSRGITLY
                     LEPKFLYNSPLAKAFVPDGFAVPFGKARLRRPGTDLTIVAYGTPVHWALEAAGTLAKE
                     GKEVEVIDLRSLAPLDFDAIADSVRKTSRLVIAHEDKVQGGFGGEIAARVTSELFEYL
                     DAPVERVGSTFTPVGFNRILERAILPDSARVLSAARKVLAY"
     gene            32248..36729
                     /locus_tag="PLP50_03840"
     CDS             32248..36729
                     /locus_tag="PLP50_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA50713.1"
                     /translation="MHPGPNGSSKGTPERTRPTRALRLVAPVLALALLHVAPPARALD
                     PARSLAQSTLDTWETGQGLPQNTVQALAQTPEGYLWIGTQEGLVRFDGTQFTLFDHRT
                     NAELSVSSVFALLADLDGALWVGTNGGGLLRMTGSRFETFGPETGLTGLKVQALARDG
                     RGVLWVGTVDRGLFRRVGGRFEKVRVGAGPHELPVRTLCVDRSGAVWAGTMGSGVSRL
                     DPGGPVSFTTREGLPNDTVIALAAHPAGGIWVGTAGGGLARIADGRVAPGGAPALPTG
                     QVSALLVDDAGNLWAGTWGRGVCLVSGENVACQSARTGELPSDHVWSLFQDRERSLWI
                     GTWTDGLLRLRHGVFASYGRREGLAGENVRAVAEDASGTIWATVASGGLQRITGTSIR
                     VFGPKDGLPSDQASSILPARDGRLWVGTYTDGLAVGRDGVFRRVALPPPEWAARDVRA
                     LVEDEDGSIWVGLLPGGVVRLRGGEARRFGVADGLPSDRLLCLHPARGGGVWAGTTAG
                     LARLEGDRFRSFTKQDGLPDERIQALALDDEGTLWIGTGNGLARLRGGTIAAVGTAEG
                     LFDSVIKSVVLHEGWLFMTSNHGVFRVRRAEVSAVMDGQRRRFASEHFGLADGMRSEG
                     CSGGTLPGAVRASDGRIWIATTRGLAVLDPERLERPTALLSVRIEGVRADGAPIEPGE
                     TVVVPPGSSRLEIRYTGISLVAPADVRFRFRLDGVDPAWVDAGRGRSASYTNITPGSY
                     VFEVAARRGAGPWTTGAKTTVQIEPTLAQKALFRAAVAGGAVALLAFALFRRGREQRQ
                     RRARLEELVAARTAELETATREAEDANRAKSAFLATMSHEIRTPMNGVIGMTSLLLGT
                     PLTREQRQFAETIRQSGETLLHLIDDILDFSKIEAGRMGLERRPFEVRPCFESAVDLL
                     APRAEEKRLEMTLDVDDGVPVALLGDVTRIKQVVMNLVGNAVKFTDAGEVAVTVVSRP
                     AGSPPPGTLVEIEVAVRDTGIGIRPERAASLFESFRQGDSSTTRRFGGTGLGLAISRR
                     LAELMGGSISFESAPGHGSTFRFVARLEAAMPPSGAIVPSRRASFAGRRVLVVEDGAT
                     SRAILAKVLSGRGLDVTACPSVAAAAERLRSGERFDGAVVDRDLPEGDGLAAFTAARS
                     RHAPSLPFVLLVPMGAETEDGADGPAARVTKPVKPAALVEAVAALVGGGRLAQKRESA
                     IRPRFDGSMGRRLPLRILLVEDNPTNQELALLMLGRLGYSADVATNGREAVEAVAARV
                     FDLVLMDIQMPELDGIEATRQIRAALGADGPRIAAMTAHALPGDREACLAAGMNDYLV
                     KPIGISDLVAALERAATALSPLAEGDDDATVAPAPLPPPPLATPPGLDGKAWARLHAS
                     LGARAGELLPGIVRSFARESDGLLASARAAAAAERADELHRAVHTLRSTSASFGALDL
                     SALCREAEARAKQNETTTLEPLLDRIGHELVQVRKVLGAIPGDGPMDEPVPSPKSTD"
     gene            complement(36742..38343)
                     /locus_tag="PLP50_03845"
     CDS             complement(36742..38343)
                     /locus_tag="PLP50_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA50714.1"
                     /translation="MESALAPASGTRTPGSQSKAASESRPDSGSLRPEDTDPGFRCLA
                     ELAPFGILVVGRDGRFRWANASAVEALGTTLDGLLASRFEAIADPSCRDLLRQRLAER
                     LTGESPPRTYEALFLRADGTSLAVEVTGNRVTWLGEVCDLIFFADVTSRRNAQAALEE
                     ERETLRSIFENVGEAVFLTLPEGPVSAANPAACRMLGRTREEILQLGRSGVLDASDPR
                     LAAALEERARTGRFRGELRGLRKNGETFPAEVTSDLFTDARGRARTATIIRDVTERRR
                     TEEALRRSEALVRALNAELERRVEERTAALASANEELRSFSSTVAHDLTAPLRGINGL
                     AGRLLEDYGARLDEEALGLLRRIGAASARGGKLIEALLHHARISVAKLEREAVDVGAL
                     AAAILRRHAAEEPERRVAWSTPSGLVLRADRRLVGEVLEHVLSNAWKFTSPREHARIE
                     VGSETERGETVVFVRDDGVGFDPSHAGRLFGLFERAHAESELPGLGVGLALAESAVRR
                     HGGWIRIDGAPGAGATVRFTLPPRS"
     gene            complement(38379..39335)
                     /locus_tag="PLP50_03850"
     CDS             complement(38379..39335)
                     /locus_tag="PLP50_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPA50715.1"
                     /translation="MPDLPASIPVPARPVHTYSIVARDPATGQLGVAVQSHWFSVGAL
                     VPWAEAGVGAVATQSFVDASYGPLGLSLLKAGRGAPDALAGLLAADAGRDVRQVAMVD
                     AAGRVAAHTGARCVEAAGHHVGKDFSVQANMMRNASVWPAMAKAFEGTKGDLAERMLA
                     ALEAAEAAGGDIRGKQSAALIVVSGAPTGRPWQDRLFDLRVEDSPAPLPELRRLVTLA
                     RAYALMNEGDLAVERKDDAAALKAYSAAQAIVPGNAEMTYWTAVSLVGMGKVDEALPL
                     FRKVFAIDRSWAEMIPRLTKAGLLPDDPSLLSRIVAEAPSPR"
     gene            complement(39333..41357)
                     /locus_tag="PLP50_03855"
                     /pseudo
     CDS             complement(39333..41357)
                     /locus_tag="PLP50_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
     gene            complement(41554..42558)
                     /locus_tag="PLP50_03860"
     CDS             complement(41554..42558)
                     /locus_tag="PLP50_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50716.1"
                     /translation="MKRIASLALASLLATALPAAAVRPADDALALVPADAVTAGMLRV
                     SDLRTSPLAARFFDEMDRATVDGDAARFLSEARLHPVDDVDLVVFAATNGGTGRALVA
                     FEGRFEPRRLAAAAVARGAEARTAAGATYFLLKDDRQTGKRGGGAVAFVSDRLLVAGS
                     EEGVAAALAARAAGGTGFDRGAGLGALRDRVDGNASGWLLVDRTKLPEAKGSVTISGN
                     DGAVRNVIGAMNLVSAFTFELMVKGDSVRLSGAGYSDDTETRELLEDALRGVLAAVRI
                     GAQEKPDVVSAIRKFKVASDRNAVTISGTLDGATIQALAAAKAEREKARAKGAHATTK
                     "
     gene            complement(42561..43415)
                     /locus_tag="PLP50_03865"
     CDS             complement(42561..43415)
                     /locus_tag="PLP50_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPA50717.1"
                     /translation="MKVLAANVEEVFREAAARWTLVAYAALSTLFIALFAAAVNLDVV
                     DGALAGAKLFGKQLEMGPRSVDLDKLVLGFESGFSGFLYVVATFLAVFATAHLVPRLQ
                     EKGTIDLYLSRPVGRVPLLLSRYVGGLLLAGLNVVYLVGAMGLLIWWKTGVVHPRFFL
                     GGAIILFTIATLMAFAFLVGVVTSSTAVSIMATFAVFFLAAILSGHRQIEAALATEWA
                     AALVRGFYWVLPKTGQLAQATVDFVSGGQLSRHKVEIDYLAVFGTTGLFGLVSLALSA
                     LFFRRKDF"
     gene            complement(43412..44392)
                     /locus_tag="PLP50_03870"
     CDS             complement(43412..44392)
                     /locus_tag="PLP50_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50718.1"
                     /translation="MTAVLSAEGLSKTFRTPFSRRQVRALDGLDLQVAAGEVFGLLGP
                     NGAGKTTTVKILLGLTHPTSGRAELFGLPVSDPGSRRRVGYLPEGHRFPGYLTARQTL
                     SVFGRMSGVSDSDLKARIPELLARVRLSDWGDVKVKRFSKGMTQRLGLASALVHRPDL
                     LLLDEPTDGVDPVGRREIRDLLREEAARGTAVLLNSHLLSEIEATCDRVAVLRKGKAV
                     AAGRIADLTAQGFAYRFVGAPMDEALLGLFRESGAAAERVNGHVKLTARDAAHLNALV
                     DAARARGVLVTELTPLRSTLEDVFVDLVRAGETDEAGGTVITPPEEGRPR"
     gene            complement(44464..45681)
                     /locus_tag="PLP50_03875"
     CDS             complement(44464..45681)
                     /locus_tag="PLP50_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPA50719.1"
                     /translation="MRRSFPGARSGARGRRRTRSCGTGGLATFAAFVLVLVVTGASTA
                     GLRADGSRPAPSSPFAAGVDTLLLVGDAGAPAAAEPVLEALAAAAARAPERTLVVFLG
                     DNLYPNGLPPEGAASRAEGERRLAAQADAALGAGARVLFLPGNHDWDGMREDGLAAVR
                     RQERFLVARSPRLLFAPGDGCPGPLLLPTPSGITLAVLDTQWWLQHEGPRPEGAGSPC
                     SAKDAEEAMAQLKTAASAGPLALLTHHPLASGGPHGERTRDWKAHVFPLRDAHPSFWV
                     PIPGLGSAWVAYRGAKGTGQDLASRRYRRLRSDLASALAGRPALFQASGHDHSLQVLE
                     APVPEARWVLISGSGIFPSGRVVSKLASTRYATSRAGFLRARLERGRAPHLEVLEVTS
                     EGKTEERLSLDLR"
     gene            complement(45678..46490)
                     /locus_tag="PLP50_03880"
     CDS             complement(45678..46490)
                     /locus_tag="PLP50_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPA50720.1"
                     /translation="MKWVVFNQKGGVGKSTLVCNLAAVLADEGERSLVVDLDPQGCSS
                     HYLLGDRAKAARPNLADFYERSLEIRLVDDGIRTFIHATPFEGLDVLPTQPVLETFHA
                     KLEGRHKIYRLRDALAPLDSYAHVFFDTPPALNFFTLSALIAAERVLIPFDCDDFSRR
                     ALYGLLDVVEEVRQDHNSKLEIAGIAVNAFQPRAKLPQLLVDELKAEGLPVLSPYVSA
                     SVKVRESHQEATPLVHFAQSHKLAGELRQLWAGLRKKARGAAKGAAPKVAAR"
     gene            46593..46865
                     /locus_tag="PLP50_03885"
     CDS             46593..46865
                     /locus_tag="PLP50_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPA50721.1"
                     /translation="MPVFEYRCQACGKKTEDLVLAGDAADYVPECAHCGSHDLSRLLS
                     AFAAHGGDKGAGSFDAPCGMGACETGDMSQCGMGGCGMGGGDFDLN"
     gene            46975..49641
                     /gene="acnA"
                     /locus_tag="PLP50_03890"
     CDS             46975..49641
                     /gene="acnA"
                     /locus_tag="PLP50_03890"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPA50722.1"
                     /translation="MSDSFKTKTTLTAGGATVSYYSIETLAKEHPEVRTLPYSLKVLL
                     ENLLRHEDGRVVKREDVLALAKWDPKAEPDKEIAFHPARVLMQDFTGVPAVVDLAAMR
                     EAIVAMGGDPARVNPLSPADLVIDHSVQVDNFASSLAFGKNVDLEYERNRERYEFLRW
                     GQNAFRNFRVVPPGTGICHQVNLEYLAKVVWTNEEGVAYPDTLVGTDSHTTMINGLGV
                     LGWGVGGIEAEAALLGQPVSMLIPEVVGMKLHGKLPEGATATDLVLTVTQMLRKKGVV
                     GKFVEFYGPGLAALPLTDRATIANMAPEYGATMGFFPVDGETVRYLEFTGRKALAPLV
                     EAYSKAQGLWRSEDAPEPRFSDTLELNLADVVPSLAGPKRPQDRVALKDAKKVFREQL
                     PAMLGKGGSADATVPVSDGRLKYEMPHGAVVISAITSCTNTSNPSVLVAAGLLAKKAV
                     ERGLDTKPWVKPSLAPGSKTVTDYLTAAGLMPYLEALGFHVVGYGCTTCIGNSGPLPE
                     KVSEAVRTGDLVVASVLSGNRNFEGRVNPQVKMNFLASPPLVVAYALAGEMDTDLTTE
                     PLGIDRNGQPVFLSEIWPTPQEVAETIARSLTAEQYEKAYGSVFAGDAGWQALSVPKG
                     STFAWDAKSTYVRKPTFFEDLGKEPKPLADVKGARCLALLGDSITTDHISPAGNIAKT
                     SPAAKYLTENGVQPADFNQYGARRGNHEVMMRGTFANIRLKNLMLPGTEGGVTLHVPT
                     GETMSIYDAAMKYQASGTPLVVLAGAEYGTGSSRDWAAKGTMLLGVKAVIAKSFERIH
                     RSNLAGMGVLPLQFAAGEDATTLGLAGKETFEVAGVAEGLTPHKQLTVTATAEDGTKK
                     TFTATLRLDTPNEVDYYRHGGILQFVLRQMTA"
     gene            49755..51158
                     /locus_tag="PLP50_03895"
     CDS             49755..51158
                     /locus_tag="PLP50_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA50723.1"
                     /translation="MGLFSDGPKGTSPELRQAIEDARRRLEESPTDAAAVLKLADALV
                     ASGRKTEAVRVLNRFGPIVQSKGRLEAAIAIFKKATQLDPDSELTSSTYLSHLQLQQI
                     LDAEKAVRAATSRPASPPPSGSFTRPGPDPLPPSGSAFELAAAPPVAEPPAGPPPSAP
                     LPRTAAAVPASDDSDAASAPAPSGPSAWSQKKEAVHDAQAGIPLLRDIPPFLLDLVLQ
                     RINLITLAPGDVLIREGAEGSSVYFVVQGTLAVTARHDLGTEVLLRTARDGEVIGEAA
                     FLTGLPSYATVTAREPSNLLELDRNALVPIARKNRTLADALNRLYEERVLHSALVRSR
                     VFGVLSEPELRLLARKLETTAVPAGTLVAQEGVPAKGAWIVKRGAFRVTFRADGHEVA
                     VALLRPHEVFGDLAEGRDLPQAESVTAIAESELLFLPAEDLSTFRTRSVRLSEALDAL
                     RLERAKMCVAALRASRR"
     gene            51224..51628
                     /locus_tag="PLP50_03900"
     CDS             51224..51628
                     /locus_tag="PLP50_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HPA50724.1"
                     /translation="MAGIKGIEGIGDTYGEKMKALGIRSKAALLKAGATKKGRADLAA
                     KAGISEKLILEWVNRADLYRVKGIGTQYADLLENAGVDTVVELAGRKAENLVEKMKQV
                     NAGRRLVRLLPALSKVQGWIAEAKKLPRAVTY"
     gene            51583..52887
                     /locus_tag="PLP50_03905"
     CDS             51583..52887
                     /locus_tag="PLP50_03905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPA50725.1"
                     /translation="MDRRGEEAPPGRHVLTDRAADETVRRGNGGRWHRGVVRRLLALV
                     LVASPAFPARAEVRAVRLSREGAGFAEGRLEAGVTVRADGLHAGRGGLYTSAAIAAPI
                     AFSAIGPYWKGAPDVAVEVAVSPDGLAWGRWIPVPAEESIAPLREDGSPNPFGGDLLG
                     PLVFVSPGSRWFRYRVRLPARRDGEPSLSRIAFQLIDPGRTADEDAPQVEARVESSLA
                     TSGPRRAVAPVPEEPSPVARADRPPILRRAAWGARPPKYAYSYTRAGHVGVHHTASVD
                     DFTPTTRAECAARLRAIQAFHMDTNGWNDIGYAWVVCRHGEVFQGREDDDDATDVQGA
                     HDGFNRGSTGLSLLGYFHPPYDQHPTQEQLSSLVDLIAWISGLRGIDPAGRSLYEAFG
                     ADVDNVYGHREVKDTSCPGDHLFALKELLRAAALEKLLIGAL"
     gene            53075..53968
                     /locus_tag="PLP50_03910"
     CDS             53075..53968
                     /locus_tag="PLP50_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPA50726.1"
                     /translation="MSRTGRIRLEAFEVLALVAFVSTVVTLLAGGLRLTPRTFWFIVR
                     ETGPFLLLFLGIGVVANVLYRLRPAGALRPYLESLRSPSWLLLTARIWVAGVLLLYAY
                     TWLKVSIPLLNERLWDAELARLDQTFHAGLSPSRFLVALFEGTPALRLLDGVYGIWLP
                     MTMSGAGFFAMTLSPRARAQFFFSYVMIWTLGVWTYVALPALGPALAYPWEWTTVHAD
                     LPQALETQALLMGNDQKVVESRVTGVLRAGFNPSLGVAAMPSLHTSGSTRCCSSGASG
                     TPARSRRCSSRRCFSLSAGRS"
     gene            53881..54114
                     /locus_tag="PLP50_03915"
     CDS             53881..54114
                     /locus_tag="PLP50_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPA50727.1"
                     /translation="MLLWCFRHARPLAAVFLAALLLTLRGSIVTGWHYAVDACVGGLL
                     AAVSFVASRRVSLHLSDPEESPPSKTPLQGVDP"
     gene            54111..55034
                     /locus_tag="PLP50_03920"
     CDS             54111..55034
                     /locus_tag="PLP50_03920"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPA50728.1"
                     /translation="MTLDISLEPALDLAARAVEEADAILVTAGAGMGVDSGLPDFRGP
                     EGFWRAYPAFRDLGLRFEQLASPHWFFRDPALAWGFYGHRLGLYRETVPHEGFGILLR
                     WASEKRHGAFVFTSNVDGQFQKAGFPDGRVAECHGSIHHLQCLRGCGEPIRSTDGVVV
                     EVDAASCRAREPFPSCPGCRGLARPNILLFGDLGWDDARTSAQMETLGDWLDGVAGPL
                     VVLELGAGASVPSVRTFSERAVQGTRRTLVRINPREPELGRGFGAPASDSLEHLLSAS
                     RQAARPSGISLPLGALEAIRAIDARRGPSRR"
     gene            55187..56584
                     /gene="vorB"
                     /locus_tag="PLP50_03925"
     CDS             55187..56584
                     /gene="vorB"
                     /locus_tag="PLP50_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPA50729.1"
                     /translation="MPALKERPKPFVLPEFCKGCGRCISACPKHCISMGTEINPKTGL
                     TPIVLDLTNCNGCGLCIDACPEPYGLQQTPADADYELQDPAKLFGDRMSDAPEPVDIP
                     DQVVPLPTMEPLVLKGNYAAAVGALLAGCRHFFGYPITPSTEGAELMAKLMPHLDGTF
                     LQAVSEVATVNMMYGCGGAGMRSMTFTSSPGFSLMLEGISYMIGAEVPGVFINVMRGG
                     PGLGNIAPEQSDIKLCCRGLGHGNTHAIVLTPSTPQEMLDLTMLAFELTFKYRNPVVI
                     ASDGYLGQMTGKVSLPESMVKPGIPKWAVYGDLEHRRNLISSIYLSEVDLEKWNVVLN
                     AKYASMQVEQRADLYMCDDAEVLLVACNTPARTAKGAVQVLRSRGIKAGLFRPVTVWP
                     FPIEKLKTVLPNAKRIVVVEASNGQLEDEVKIALANARIREYPEIEHVRHFGGVLPQM
                     EEVILKVAAGQEVTV"
     gene            56581..>57568
                     /locus_tag="PLP50_03930"
     CDS             56581..>57568
                     /locus_tag="PLP50_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPA50730.1"
                     /translation="MTTSAFYGKFERHSHGEGLKGHSTHYCPGCGHGLAHKYLGEAID
                     ELGIQDRTVAVSPVGCSVFLYYYFDVGNTQAAHGRAPAVAIGQKLANPESIVISYQGD
                     GDLASIGLAEIVSTAQMGIPITVIFVNNAIYGMTGGQMAPTTLMGQKTATSPDGRVDF
                     MGQPIKMAELIAGLDGPVYVERVALFNPKQRNRAKKAIHKALQNQVEKRGFSFVEVLA
                     ECPTHLKATPEQAEKWVEEAMLPMFPLGVKKDETREPWWDRPAPTFETERVLKEIGAG
                     EEKAPRYCTSFPSHVAKNDISLKLAGSGGDGAQTAAMLIVHAGINEGFDATHI"
BASE COUNT         8145 a        21328 c        20026 g         8069 t
ORIGIN      
        1 agcggatgac gaggcccgtg tccttcctca cgaactccgc gagctcgttc ccgcgtcgcg
       61 cgagagttcc ctgctcgacc gaggggacga agagcatccg gatgacctgc tctcccgccc
      121 gctcgatccg gcctcggcgg acgaggcccg cgacgaacac gaggaacagg agcgcggcga
      181 ggagccagcc gagcctcggg aggagggctc cgcgtcccgg tgacggacgc gaccccgccc
      241 gggccgcttc tcctcccctc ctcatcccga tccgggagtc taaggcatcc gtgtgaagcc
      301 cccgccgcgc gtcgctcccc cggattcctt gacgccctct cggcgccggg gctaccgtcg
      361 cgccggatgg acacgccgct cggtcgcacg ctcgccgtca cgaaggcgct cgcgaacccg
      421 gcccgcctcc ggatactcgc catcctcgcc gggggcgagt gctgcgtctg ccagctgacc
      481 gcagttctct ctctcgcacc gtccacggtg tcggcgcacc tctcggagct tcgccgggcg
      541 gggttcctcc tcgtccgtaa ggctgggaag atcgtcttct accgcctcga cgagcgcgcg
      601 gccctttcgg tctggaggag ggccgcgctc gccggactcc acgacgacgc ccgggtcgcc
      661 gaggaccgag cgctgctcga gagggtgaag acggtcgacg tcgagacctt ctgcggcgcc
      721 gggccccggt ggaggctcct ccccgcgttc cgcccgtcga agggcgcggc cgctccgcgc
      781 cgccgccgct gacgcccggg gcggcgcgcc gagagcctcc ctatcgcgcg cttcgcaccc
      841 cccagagagt ccgtcccgcg acggcggcga ggacgattcc gcctccgagg agggcccagc
      901 ccgaggggcg ctccccgagc ccgaggaacg cccagagcgg gttgaaggtc ggctccagca
      961 tcccgatgag cgacgcctcg gcggccggaa cgaccgagag gccccgaacg aagaggatgt
     1021 aggcgagtcc catctgcacg acgccgagga agacgatgac tccgagcccg cggccgtcga
     1081 gcgagaggtt cccccaggcg aacggaagcg cgaggagcgc ggcgaggacg ttcccgagga
     1141 agaccgaggc catcgcgtcc cgcgactggt cgcggcgtag caggacgacc gtgagcgcga
     1201 agaagagccc cgagacgacg gcgaggaggt ttcccgcgag atcgccgccg tcgagccggc
     1261 cgacgaagaa gagcgacatt ccgccgaggg cgaccgcgat cgcgacggcg tcctctttcc
     1321 ggaagcgctc cttcaggagc cacggcccga gcgcgaggac gtagaacggg cccgtgtact
     1381 ggaggaagat cgcgttcgcc gccgtcgtca gcttgttggc cgagacgaag cacaggatca
     1441 tcagggcgta ggagagcgcc gtcgccagcg acgccttctt ccacctctcg atcccggggc
     1501 gatagacggc cgcgaggaag aggaacgtga tcgacgaccg ccagaagacg acgccgacgg
     1561 cgtcgagcgg gacgagcttg atcgcgaggc ccgccgtcga ccagaggagc gccgcgccgg
     1621 cgatggagag gcggccggcg cggggggcga gcggggcgtc cattcagcgg ccgaggacga
     1681 ggaccgggac ggtgagggcg agggcgcgct cgggaaggca ggtcccgtcg ccgcaggcgc
     1741 gatagcggag cgagatcgcg acggggtgct ccccctccgc ggccccttcg cgcacctgga
     1801 agacgagcgt cacgccgatc cgcccctcgt agcccggctc ggaggagccg tccgcgtgtg
     1861 cgaccgttcc ccgcggcggc agctccaccg cgacgcagtg cagccccgga ccgccccagg
     1921 cgtcgacggc gagcggcgac tccccggtca gggcccagcc ctctgcgacg tcgatcgcga
     1981 tccgcacctc gagcatctcg cccggtgagg cgcggtcgga ggagagggag gccgcgaccc
     2041 ggaccgggcc gctcgacgcg ggcgtctcgg cgggaaccgg cttcgagcgt cccgattctt
     2101 cgtcgtcggc cgtgtccctg cctccggctt ccgcctcggg agccgccccg gcaggtcgca
     2161 ggaggccgct cgttcgaagc tcctcccacg ccaggtgcca cgactccgtt ccgtgcggga
     2221 cgcgctccat cagaccagag acctcctcca cctccgcgag cgccgcagct gcgagggccg
     2281 cgtcgccgtc gcggcgggcg aggcgggcca tcgcccgcgc cgccgccgcc ggcgcggaag
     2341 ggatcgcctt gtcgaagagg tcgcgcgttc gcgcgaagag ccgctcgtgc ccttcgcccg
     2401 tgaacgcgag cccggggact cccgggcggc cgaaccgggc gcggaggccg gcgacggtcc
     2461 ggcgtgcctc ggcggcccac cgcgccttgc cggacgggtc gggctcggcc tcggcgaggt
     2521 cgaggagcgc gagagcgagg aacgcctcgt cctcgagaaa cgccgggatc cgggcctcgc
     2581 cgtccttcca ggtccggtag aggcggcctc cggcgtcgcg catccgcgtc aggaggaact
     2641 ctgccgaggc ccgagcggcg tcgaggtagc gcggctcgcc gaggatcgac gacgcgaccg
     2701 cgagcccgga gacggcgagg gcgttccagc ccgcgatccg cttgtcgtcg gtccccggcg
     2761 ggacgcggag gcgccgccgc tcgagcaggc gcgcgcggat cggcgcgagc cgccgctcct
     2821 cctcggacga gggctcctcg gcgaggtgga ggacgttccg gcccgtcgcc cgcccggtcg
     2881 actcgtcgtg gaagttccct tcgggccgga cgcggtaggc ggaggcgaag agcgccgcgt
     2941 cgtcgcccgc cacggcccgc acctcctcct ccgtgaagac gaagtagcgc ccctcctccc
     3001 cttccgagtc ggcgtcggtc gcggcgaaga agcccccctc ctcgccccgc atctcggaga
     3061 ggagccagtc cccgatcccg cgcgccgtcc gccagtagag gtcgcgcccc gtcaggacga
     3121 acgcccgcgc gtagagcgcg aggagctggg cgttgtcggt gagcatcttc tcgaagtgcg
     3181 ggaggagcca ccgctcgtcg gtcgagtagc ggtggaagcc gccggcgagg tggtcgtgga
     3241 tcccgccgag ggccatcgcg tcgagcgtct tcagcgccat cgaccgcgcg atcgaatccc
     3301 cctcgacgcc cttcaggagg agccattcga gggcgaggtg gggcgggaac ttcggcgcgc
     3361 cgccgaagcc gccgtgccgg gcgtcgaacg tccgcttgag cgtctcggtc aggacgcttc
     3421 ccgtccgcgc cgtcagcggt tcacgcccgg cgcgatcggt gatgcggccc gcgcgctcca
     3481 cctccgcgac gatctcgtcg gcccccttcg aaagctcctc gcgccgctcg cgccacgcct
     3541 cggagagctt ctcgaggatc gtccggaacc ccgcctggcc gcgccggtcc tcgcgcggga
     3601 aatacgtccc gccgaagaac gggcgcccgt cggggaagag gaacgccgag agcggccagc
     3661 cgcctcgccc gaccatcgtc tgcaccgcgc tcatgtagac gtcgtcgacg tcgggccgct
     3721 cctcgcggtc gaccttcacc gacacgaacc gctcgttcag gagcgccgcg accccctcgt
     3781 cctcgaacga ctcgtgctcc atgacgtgac accaatggca ggcggaatat ccgatcgaga
     3841 ggaaaagcgg cctgccctcc cgccgcgcct tctcgaacgc ctcctccccc cacgggtacc
     3901 agtcgaccgg gttgtgcgcg tgctggcgca ggtagggaga cggctcgttc gcgagccggt
     3961 tcgggggacg ggacgggtgc gtcattccgg gattctgccg cacccgcccg gcccgtccgt
     4021 cagggcccgc cccgccgccg ccggacggcc ttccaggcct ccacgacgag gaggaccgcg
     4081 aggccggagc cgaacgcgat cccgaggtcg cgcgcgccga gcggcgcggt cgagaagacc
     4141 ctctggagga acgggacgta gacgacggcg acctgcaggc cgaccgtcga aaggaaggcg
     4201 gccaccatga gcgggttcgt caggaggccg agcgtgagga gcgaggcctt ctcggacctc
     4261 acctcgagcg ccaggatcac ctgcgagaag atgagggtcg tgaagaggag cgtctgccag
     4321 gccgggtctc cgctccggta ggcccaaagc ccgatcccga gcgcgatcag gctcatgacg
     4381 acgcccatca cgaggacgaa cgggacgagc cctctcccga agatgctctc ctccgaggag
     4441 gccggcgggc gcttcatgat gtccgcctcc gccggctcta cgccgagcgc gagcgcgggg
     4501 agaccgtccg tcacgaggtt catccagagg atctggagcg gcaggagcgg cagcggcatg
     4561 ccgaagagcg gcgcgagcag catgaccgcg atctcgctcg cgttgcagct gagcaggtac
     4621 ttgatgaact tccggatgtt gtcgtagatc ttccggcctt cctcgacggc cgcgacgatc
     4681 gtggcgaagt tgtcgtccag gaggaccatc tcggcggcgt cgcgggagac gtcggtgccg
     4741 gtgatcccca tcgcgacgcc gatgtcggct ttccggagcg cgggggcgtc gttcaccccg
     4801 tcgccggtca tcgcgacgac ctggccctgc gcctgaaggc tcgcgacgag ccggagcttg
     4861 tgctccggcg agacgcgggc gaagaccggc acctcgccca ccgcccgtgc cagctgcgcg
     4921 tccgagagac gatcgatgtc ggctcccgtc cggaactcgt ccccctcggt gattccgacc
     4981 tgcttcgcga tgtggcgcgc cgtgagcggg tggtcacccg tgatcatgat cggccggatc
     5041 cccgccgcgc ggcagcgcga gaccgcgtcc gcgacctccg gtcgagccgg gtcgagcatc
     5101 ccgaagaggc cgacgagcag cagctcctcc tccagcgagg cgagctcggc ggcggagggc
     5161 gggccgtcga gcggccggac ggcgagaccg aggacgcgca tccccttcgc cgcgaggtcg
     5221 tcgtgcgcgg ccatcaccct ccggcgcgcc tcgtcatcca ggggcacggc cgactccccg
     5281 gaccagacgc tccgcgtctt tcccaggagg ccgtcgatcg cccccttcgt cacggcgagg
     5341 taccggggcg tctcctccgg gaggacgcgc cgcgcccaga gcgggcgaag ggagggaggg
     5401 agctccgcgt cgccggcggg gagctggtgg accgtcgtca tccgcttccg cacggagtcg
     5461 aactggagct ccgcgacgcg cgggagcgcc cggtcgagct cgtccttccg cgcgccgtac
     5521 tcggcggccg cgaggacgag ggagccctcc gtcgggtccc cgacggcgtg gaacgccccg
     5581 cccgcgcctt cgtccttcac gagggaggcg tcgttgcaga gcgcgcccga ggcgaggagg
     5641 aggtcgagcg tcggagcgag ctccggctcg gcttcgccgg ccccgacccg ccgaagcgag
     5701 aagtgctccg ttccttcgcg ctggacgagg tcgatccggt ggtccgcgac gtcgagcgcc
     5761 gtcacggtca tccggttctg cgtgagcgtc ccggtcttgt ccgagcagat gaccccgacc
     5821 gagccgagcg tctcgacggc cgggagcttc cggatgagcg ctctgcgccg aagcatcctc
     5881 tgggcgccga gggacagggc gatcgtcacg acggccgtca tcgcctcggg aatcgcggcg
     5941 accgcgaggc tcaccgccgt caggaggacc tcgaagacct cttcgcgcgt gccggccgag
     6001 cggattccga ggaggaagac gacggcgacg aggacgcccg ccccgatcgc gagtcccttt
     6061 cccatccggt cgagccgctg ctggaggggg gtcttctcct ccacgaccga ctggatgagg
     6121 ctcgcgatct tcccgagctc cgtcgccatc ccggtcgccg tcacgacgac ctcgccgtgg
     6181 ccccaggtga ccaccgtccc cgcgaagacc atgttccgac gctcggcgag cggcctctcc
     6241 ggctccagga cgaccccggc gtccttctcg accgcctccg actcgcccgt gagcgcggcc
     6301 tcctgcgccc ggaggttcac gctcgaaagg acgcggccgt cggccgggac gacgttgccc
     6361 gtctcgagga ggacgacgtc acccggaacg aggtcccggg aggagatctc ctgcaaccgg
     6421 ccgtcgcgcc gggcccgcac cgtcggcacc gacatccgct tcagcgccgc catcgatcgc
     6481 tcggctcgat actcctgcga atacccgagg gcggcgttca ggaccacgat cgcgaggatg
     6541 acgaccgcct cgacccagtc cccgagcacg accgaaaggg ccgcggcgac gacgaggatc
     6601 accgtcatca cgttcagaag ctgctcgcgg acgatctcga gccggctccg gccggcccgc
     6661 tccgtgagct cgttcgagcc gtggcgcccg aggcgtgccg ccgcttccgc cgacgcgagc
     6721 cccgtccccg cttcgctgcc gacgtccgcg aggacctccg cgaggctccg ttgccaggcg
     6781 ccggccgaac gaggcgcgct ctccatctcg ggctcgagta tgccccgcgc gggcgggacc
     6841 cgcgcgcggg gctcccgctt tcgcgaacgc ctctgtagga tcgcgcgcca tgaccgcaaa
     6901 cccgcagcgg ccgcacacgc gcccgacgtc gaccgtcccc gccctccgac acgccctccc
     6961 gctccttctc tgcgcgctcc tcaccctggc cgtcgcacgt ccctcgccgg cggccgcgcc
     7021 ggagaaggcg ctggtctcct gggacgacgc gaagcgcatc tcgatcgacg agctcgcccg
     7081 cctcctgaag gaccccacgg ccgcgaagcc ggagatgttc cacgtcggct tccgggtcct
     7141 cttcgtccag gcccacatcc ccggctcccg cttcgctggc cccggggcga gccccgccgg
     7201 cctcgagacg ctccggaagg ccgtggccgc ggtaccgaag gcaaagacgg tcgtcctcta
     7261 ctgcggatgc tgtccgtggg agaagtgccc gaacatggag cagccgtggc ggctcctcgt
     7321 cgcggccggg ttcaccgacg tccgcgtcct ctacatcccg acgaacttcg gccgggactg
     7381 ggtcggaagg ggccatccct cggcctcggg aagcggcgac cgtggctgag cggctccgtc
     7441 gtctccgggc gctcggaggc ctcgtcgcct gcgccgcgct cctctctctc gccgtccggg
     7501 ccgaggaccc gcccgcgccg aagatcggcg acccggcccc cgccctcggc ctcgtcgacc
     7561 tcgacgggaa gaagctcgat ctctcctcgc tccggggcaa cgtcgtcgtc gtcgacttct
     7621 gggccacctg gtgcgcgccc tgtcgctccc aggccccgag gttcgaagcg ctgcaggaga
     7681 agcaccgggg ccgcgggctc gtcgtcgtcg gcgtctcgct cgacgagtcg gccgagccgg
     7741 tcctgaagtt ccggaaggag ctcggcctga cgtaccggat cgtcctcggc gacgagacgg
     7801 tggccgagcg ctggggcggc atcctcgggc tcccggtggc cttcgtcgtc gaccggcagg
     7861 ggcgcctgcg cgcgcggcac gagggcgagc acgacctcgc cgccctcgag gcggacgtcg
     7921 tccggcttct cggagagaag tagcgccgtc aggcgggccg cgacaggcga acgcgcgcga
     7981 tccgccgggg cgtgctttcg acgacctcga tcgtcaggcc cgactcctcg tcgacgaacg
     8041 ccgtccccgc cggcgggatc cgctcgaggc gatggaggat gagccccgcg atcgtcgccc
     8101 agcccgggtc ctccggcagc tcgaggtcga gcagccggtt cacgtcgcgg acgccggcgt
     8161 gcccctcgac gacccaggcc cccgggccgt cctcgcggta cggcacggcg tcggcttcct
     8221 tctcgtggaa gatctctccg acgagctcct cgaggaggtc ctcgagcgtc accaggccgg
     8281 tgaacccgcc ctgctcgtcg acgacgaaac cgatcgggac gtgccgctcc tggagcgcgc
     8341 gaaggacgcc gagcgcctcc acggtctccg ggaagaacgg cgcctcgcgg acgattccct
     8401 gaagcggcag cgtcgcgccc tcgaggatgc gcgcgaggac ttcccggccg aggacgtacc
     8461 cggtcacgcc gtcgagcccc ccgtcgtgga ccgggaagcg cgcgaaccgg ctccggctga
     8521 cggcggagac gatctcctcg cgcgtcgccg tcgcgtcgat cgcgacgacc gagggccggg
     8581 gcgtcatcac cgctccgacc ttcacccccg gcaggtcgag cgcccgcgcc gcgatctccc
     8641 ccgccctcgg gtcgagccct cccgtcgtcg ccgtctcctc gacgagcgtc cggagctcgt
     8701 ccctcgagag ccgggcctcg acgaacgacg tcctgtcgcc gaagagtcga agcaccccgt
     8761 tcgagcaggc ggtcaggaag cggaccgccg gccgggcgag gcgggcgagg agtgcgaggg
     8821 gccgggcgat cgcgagcgcg tacctctccg cgaagcgcat cgcgagcgac ttcgggacga
     8881 gctccccgac gacgagcgac aggaaggaga cgacgacgac gacgaccgcg agggcgacgt
     8941 cgtccgccag ctcccgcggc gccccggccg cttcgagcgc cgccgcgagc ggagaggaga
     9001 gcgtcgcccc gccgaaggcc gcggccgccg caccgacgag cgtgatcccg atctggacgg
     9061 tcgcgaggaa cccctcgggg tcggcccgga gcgccgccag cgccttcgcg ccggtccgct
     9121 cctcctcgac gagctcgtcg aggcgcgtgc ggcggagcgt caggaccgcg atctccgctc
     9181 ccgcgaacag gccgttcacg accacgagcg cgaggacgac gagaaccccg gtcacccgtc
     9241 gattctaggg gccggcaaac aatacacgat ttcacgcctg gcaccgggag tggggcaccg
     9301 ggagtggggg ccgggtatcg tccggggacc ggatggaacg gacgacgctc ggcgagatcg
     9361 cggcggtgtt tctgcgcctc ggcacgaccg ccttcggggg tccggcggcg cacgtcgcga
     9421 tgatgcgcga cgaggtcgtg aggcggcgcg ggtggctgac cgacgcggag ctcctcgacc
     9481 tcctctccgc cgcgaacctg attcccgggc cgaactcgac agagctcgcc atccacgtcg
     9541 gctgggcccg ccgccggtgg gctggactcg ccgtcgccgg cctctcgttc atcctccccg
     9601 cggcgctgat caccgccgcg ctcgcctggg cctacgtccg cttcggggcg ctcccggagg
     9661 cggaccgcct cctctggggc gtcaagccgg tgatcctcgc ggtcgtcgtc caggcgatcg
     9721 tcggcctggc tccgcacgcg gcccgcaccg tcccgctccg gctcctcggc atcgtcgccg
     9781 cggtcgcggc cgcgctcggc gcgcacgagc tcgtcgtcct tctcggcgcc gggctcctcg
     9841 tcgcggtcgt gcgggccgtc cgctcccgcg gcgccggtgg gaacgccgcc ctccccgcgc
     9901 tcgggccgct cttccccgtc gcggccggcg ccgccggcct ctccgcggtc ggcgccgtca
     9961 cgctcccggc gatcttctgg ctcttctgcc ggaccggggc cgttctcttc ggcagcgggt
    10021 acgtcctcgt cgcgttcctc cggaccgacc tcgtcgagcg gctcggctgg ctcacggagg
    10081 cgcagctcct cgacgccgtc gccgccggac aggtgacgcc cggccccgtc ttcacgaccg
    10141 ccacgttcgt cggctggctc ctcgccggcc cggcgggcgc cgccgccgcc accgccggga
    10201 tcttccttcc ggcgttcgtc ttcgtggcgc tgtcggcccc gttcgtcccg cggctgcgcg
    10261 cttcgcggac ggccggcgcg ttcctggacg gcgtgaacgt cgcctccgtg gcgctgatgg
    10321 ccgtcgtgac ggcccggctc gcgccggccg ccctcgtcga cctccgtgcc gcgctcctcg
    10381 gtctcctcgc ggccgttctc ctcgtgaggt accgcacagg ctcggtcgtt ctggtcctcg
    10441 ggggcgccgc cgcgggactc ataatcggac cgtgacgtcc atcggcgacg cattccggcg
    10501 gaagaccgcc gaggccgact ctctcctcac gcccgccgag cgggtcgcga gggccttctc
    10561 tctcggaaac gacgacgccg aggcctttcg ccgttcccgg gggctgacgc gcgcggccgc
    10621 cgaggccgag ctcgccgcga agaggcgatc cggccggatc ccgagccggg tggcgggaac
    10681 cggatgagcc tcctcgccgt cgtcgcgaag cacctctccc tccgccgcac gcccttcgcg
    10741 acggtcggag gcgtcgccct cgcgatccgc ggcgtcgccc gctcgacgtt cgacgtcgac
    10801 ctcctgaccc tcgacgcctc cgtcctcgcc gacgcctact ggtcgctcgt gacgcgcgac
    10861 ggtgcgcagg tcgagatccg gcggggcgac ggcgacgacc cgctgcgcgg cctcgtccgg
    10921 ctcgtctccc gcgacgagcg ccccgtcgac gtcttcgtcg gccgcggcgc ctggctcgac
    10981 ggcgtcgtgg cccgcgcgga gccgttcgac ctcctcgaca cgcgcgtccc cgtcgtccgc
    11041 gcggccgacc tcgtcctcct caagctccac gccggaggca cccaggacct ctgggacgtc
    11101 acgcagctcc tcgccacgga cgacggcacg ctcgccgacg aggtcgtcgc ccgcctcgcc
    11161 gaggtccccc gcggcgccgc catcctctgg cgccgcctcc aggaggcccc ccatcggggt
    11221 caggcgtgaa atcgtgtatt acgcggccga ccgcgcctcg ctccggccct tccagagcga
    11281 cgcgacgacc cccgcggcga ggatggagag gacgacggcg aggaggtaga ggttgaagtt
    11341 ctcgccgagc atcgccttca gccaggtgtg ggtcatcatc ttgaccccga cgaccatcag
    11401 gacgacggcg agcgccacct tcaggtagcg gaacgagtcg atcatcccgg cgagcgcgaa
    11461 gtagaggctc cggagcccga ggatcgcgaa gacgttgctc gtgaagacga ggaacggatc
    11521 ggccgtgatc gcgaagatgg ccgggatgga gtcgaccgcg aagatcaggt cggtgaactc
    11581 caccatgacg agggcgagga ggagcggcgt cgcgaggagc gtcccggcct tcgccgcgtc
    11641 gacgaccctg tcggcgacta ccgccgcgcc cggcgtctcc gcctcgtgcg aggcgtcggt
    11701 ccctgccctc acgaagaagt gctcgccgtg gaagcgctcg gtgaccggga agagcttccg
    11761 cgtcgcgcgg acgacgaggt tcctgttcgg gtcggtctcg ccctccttca ggaggagcat
    11821 cttgatcccg gtcaggatga ggaagccgcc gaagacgtag atgatccagg agaactcctg
    11881 gatcatccgc gcccccgccg cgatcatcat tccccgcagg atcagcgcgc cgaggatccc
    11941 ccagaagagg acccggtgct ggtagagcgc cgggacggcg aagaacgtga agatcatgct
    12001 gatgacgaag atgttgtcga cggcgagcga cttctcgacg aggtacccgg tcacgtactt
    12061 gacgagggcc gaagcgccgt cgttgtagac ggtgccgagc ccctcgaccg ggacgggccg
    12121 ggtcgtcatc aggtcgggcg tcagcccgag ccccagccag tggttctcgt acccgaggta
    12181 gatgaacccg ctgaaggcga gcccgagcgc gatccagaag gcggaccagc cgagcgcctc
    12241 ccggacggtc acgacgtgag ccttcctgtg gaagaccccg aggtcgagcg cgagaagggc
    12301 caggaccagg aggatgaagc cggcgtacat ccagaacact cgggacgctc ctttcggggc
    12361 cgacgctcgg cgccggaccc cggacggagc gctaacgcgc gaggcgccgc ggaaattccc
    12421 cgcccggacc gggaccggac ggaccgcccg cgctatgatc gcgggcctga gacggcttct
    12481 gcgatcggtc cttgcctgac gggccggcgc gccccgtagc gcgcccctga tcgagacggg
    12541 cgacccgccc ggcgatgtct gtttcgccgc ggcgccccgg ctctctttcc ccgtccgcac
    12601 accgagatgg agaagcccgc catgcccctg acccgattcg ggtgggaccg cgatttcgaa
    12661 gcccagttcg agaaccatgc gcgcgaaggg ctcgtccccg gccgcgtcct gaccgcctcc
    12721 cgcgagatct accgcctggc caccgccgcc gacgagatcc cggccgagct ctccggccgg
    12781 ctccgatacg ccgcggagtc ctccgccgac ctccccgccg tcggcgactg ggtggccgcg
    12841 cggatctacc ccggcgacgg cctcgccgtc gtcgactccg tcctgccgcg ccggtccgcc
    12901 ctcgtccgac gatcttccgg ccggcgggac gaggcgcagc tgctcgcggc gaacgtcgac
    12961 ctcctcctcg tcgccgcgcc gctcggcgga gagctgcgcg agcgaaggat cgagaggttc
    13021 ctcgccctcg ctcgcgaggc gaaggtccgc cccgccgtcc tcctgacgaa ggccgacctc
    13081 gcctacgatt ccgcggccga cctcgccgcg gcccgggccg tcgccggcga cgcgccggtc
    13141 gtcgtcgtga gcgcgcggac cggcgccggt ctcgacgcgc tcgatcccct cctgccgccg
    13201 caccgaaccg ccgccctcct gggtccttcg ggggccggca agtcgacgct cgtcaacgcc
    13261 ctcctcggcg aacgccgcca gtcgacccgc gaggtccgcg agagcgacca ccggggccgg
    13321 cacgcgacga cgagccggga gctcttcgcc ctcgcgagcg gcgccctcct cgtcgacacg
    13381 ccgggtctcc gcgagctcgc gctctgggac ggcgcggagg cgatcgccga gacgtacgac
    13441 gagatcgcgg cccgagcgcc ggcctgccgg ttccgcgact gccgccacga gaacgagccc
    13501 ggctgcgccg tccgtgcggc tgcggacgag ggcgtcctcg acccggatcg cctcgccgcg
    13561 ttcgagaagc tccgccgcga ggacgagtcg ctcgccgagc gccgcgcgct cgggccggcc
    13621 cgcgccgagc gcgagcgctg gcggcgaatg atgggtgggg cgaaggggcc ggtgaagctg
    13681 cggtgaggtg cgccgtcagc ggatcgccga ggccgcgggc tcggaaccga tcgcggcgcg
    13741 ggcgaggagc ttccccgcct cgttcgagac gaggacccag cggtcccggt gggccgtcgc
    13801 gaggatcgag tacggccgtc gccggttccc gggcgccgcc cattcgccga ggacctcgtg
    13861 atgcaggagg gcgaggaccc gcccaggtcc gttccagagc cgcgtgaact cctcgtcggt
    13921 ctgaaagagg ccggtgcggt cgtggcttct cccaaactgg agctcgctct ccgtcccgat
    13981 gcggctcagc ggcgtcagtc ggcgaacgta gaacgggagg gactcgacgt agtccccgcg
    14041 ctggacgagg aggtctccgg ggcgaagtgt ctgccggagg gccgtgacga tcggccagct
    14101 gccgtcccac cgggcccagg ccggaaggac gaggtgcgcc ccgccgagga agaggagcca
    14161 cgcggaggcg acgagcagga ggcctcgctc cgggacgagc gtcgggacga tccccacgag
    14221 caggaacccc gcgaggagcg tcgcggagag ggcgaggagg gggcccgcga ggctcgggac
    14281 ggcgaggtcc tcgaaggcgc cggcccacgc cgcggctgcc gcgaggacgc cgaggaggcc
    14341 ggcgatgctc cagcgctcgc cgggcctgag ggcgagcccc tcctcgtcac gcgccacggc
    14401 gagcgcgagg agaagcgcga tggcggggaa gacggggtgg acatagggaa tcagcttcga
    14461 cttcgacatc gagaagaacg cgacgacgag gaggacccac aggatcagga acgactcgag
    14521 tggacgcgcg aaccaccccg cgcggcggcg ccccggccag accgtccgga gacgcggcag
    14581 gagcggcgtg aaggggagga agcctcccgc gacgaccgcg aggaagtacc acggggcgcc
    14641 gggacggtga tggttcggga ggaggaagcg ctcgaagtgc tcgcggacga agtagaagcg
    14701 gaggaagtcg ggctcgcgga gcgcggcggc cacgtgccac gggaccgcga ggacgaggaa
    14761 cgtgaggacg gtccacggcg agaggagggc ccggagcgga cgcgtcctgc cggtggcggc
    14821 ggtccacagg agcaccgccc ctccgggcag gaggacgccg acgaggccct tcaggaggac
    14881 ggccgccgcg gtcgacgccg cgaatccgag cgcgagcgtc ctctcgcgcc gcaggctcgc
    14941 accgccctcg ccgagcgcgg cgaacgagag gaccgccgcg gtctgggcgg ccgtcaggat
    15001 cgggtcgatg aggacgaccc gggcgaggcc gaagccgaga agggagccgc cgaagaagag
    15061 ggcggcgagg gcgccggccc gctcccccgc ccggcggcgg gcgaagagga agagcgcgag
    15121 catcgtgccg atcgtggcca gcttcccggg gaggcgcgcc gccgcctcgc tctccccgaa
    15181 gagctcgtac gagccggcga cgagccagta ggccagcggc ggcttctcga tgtagacgag
    15241 gccgttctgg tgcgggagga cccagtcccc cgaagcgagc atctcccgcg ggatctccgc
    15301 gtagcgcccc tcatccgggt cgacgagcgg cacccggaac ggctcggcga ggacgagcag
    15361 cgcgacgaga atggcgagga gcgtctcggg caggaggcga acgcgggcgc tcggcatgac
    15421 ggtacgctcg cccaggccgt cgaatgcctt ccgaaccctc ccgaacggtc gccgaacgcc
    15481 gtcttcagct tcccgtgccc ttccagcccg ggagccgcag ctcgaagacg gtccgatccg
    15541 ctcccggctc ggcggcgacg acgtcgccgc cgtggactcg cgcgacgtgc cgcgccaggg
    15601 agaggccgag cccgtggccc ggcgccgtct cgcgcgcgga ggcgccgcgg tagaagcgct
    15661 cgaagacctg ctcgcgctcc tcctcgggaa gggagacgcc gggggtctcg acggagagcg
    15721 tcacaccgtc cgccgccgcc gtgaggaccg cccgcgggac ccgatcggcc ggcgcgtact
    15781 tccgggcgtt gtcgagcagg ttgtcgacgg cgagccggag gagctcctcg tcgccccgta
    15841 cgagcgcctc gtcgggcgcg tcgaccgcca ccgtccgctc ccccgattcc gccgaagccc
    15901 gcacgacgtc cgcgaggttt gccgtctctt tcaggtcgag gccggaggac tcgtcgcgcg
    15961 cgaggacgag gagggcgtcg acgagcctcg tgagccggtc cacctcgttg gtgacggcct
    16021 gcagctcgag ccggcacgct cccgacgcgc ccgcggccgc gcgctcggcg cggaggcgga
    16081 tgcgcgtcag cggcgtccgg agctcgtgag ccgcgttcct cacgaacgac gcctccctct
    16141 cgagcgccgc ttcgaggcgc ccgaggacgc tcgcgaagct cccttcgagg gcggcggctt
    16201 ccttcggaag agcgtcgaga ggcgccggga cgtagtcgcg cggatgctgg atcacaccga
    16261 ggcggcgggc caggtcctca atcgggcgcg ccgcggctcg ccccgcccgg cggccgacgg
    16321 cgagcgcgac gagtgacgaa gggatggccg cgacgagcag cgcggcccag aagaggcgcc
    16381 ggagggggag cccctcctcc ggccggcgtg cgacctccag gacgatgccg cccgcgaggg
    16441 cggcccgcgt ttcgatccag cccgaaggag gacgtccgtc ctcctcccgg gccgggggag
    16501 tccctcgctc ggcgaggagc aggcgccccc gatggaagcg gccccagtcc gccggctcca
    16561 gcgcctgcgc gagcgcctcg cgcgccgatt cttgggcgtc gtgctcgccc tccgtctgct
    16621 cgaggacgaa gatccgcgag gcggcgtcgg ccgtccgcga cagctcgacg gcgtcgtgcc
    16681 ggtcccagag cagccgcagc gcgagaacgg acgcgaccgc gaagacgagc gaggagacgg
    16741 cggcagtcag gagcgtcgcc gcgaccgtgc ggcgcgcgaa ggagcggacg atcacgcccc
    16801 ctccgtgtcg agcgcatagc cgacgccccg cacgttgcgg aggaggctcc ctcctccccc
    16861 ctgccgcagc ttccggcgaa gccggctgac gatgacctcc aggctcgccg cggcctcggg
    16921 cgtgtcggcc ccccagacgt cctccaggag cgcctcccgg gcgacgacga cgccgccggc
    16981 ccgaacgagg cgcgcgagga ggtccagctc gcgccgcgtc aggggaagct cctcctcccg
    17041 gaggaacgcc cgccgccggc gaaggtcgac gcgcacgccg ccgtgctcga ggacggatcc
    17101 ggacggcccg ggccgcgcgc cccgccgaag gagagcccgg acgcgagccc gtagctccgc
    17161 gagggcgaac ggcttgccga ggtagtcgtc ggcgcccgcc tccagcccct cgacgcggga
    17221 ggcgacgtcg gtgcgcgccg tcagcatcag gatcgggacg gcgctctcct cgcgccgcag
    17281 ctccgcgcag aggtcgagcc cggacgggcc cggaagcccc acgtcgagga tcacgaggtc
    17341 gaactcacct tcacgaacga gcttccgagc cgtctcggcg cgggacgcgg tcttcacgtc
    17401 gagcccgtcc tccccgagcg cgcgcgccac gaactcgcgc gtctccgggt cgtcctcgac
    17461 gacgaggacc cgcggagcgt ggtcggacat cctcagcggc ctccgcgccc ggccccgtcg
    17521 ccctttggcg gcttcacgag gagccgcgtc tcggcgcgcg ccccggagac ggacacctcc
    17581 acgacgtacc ttcccggcgg aaggtagcgg tctcgcgtcg cgcggagcgg gtcggcgagg
    17641 agcgcggcga cctcgggcgg cgtcgcgcgt ggtccggcgt ccgcgtcgtc ttcgtcgtcc
    17701 tccgtctcgg gcgcggccac cgggtccggc gactccttcg tggcagtcgt cttctccttc
    17761 gccttcgccg ccgcctccgc cgcctccttc cggagggcct cctcgttcgc gtcggcacgg
    17821 gtccggtcgg cggaaaggtc gtaggcgacg gcgttgaagc cggcgacgga gggggccgtc
    17881 cactccttcc agacgcgtcc cttcccgtcc ttcaccgtga tcgtcgcggt cccggccgcg
    17941 gcgggcgagg cccaccagga aacggtccgg gtcgtgggga cgcggcgcca ggcgaagagc
    18001 tcgttctcgc cgtagccccg gctccgctcc cacgtcgcgg gcttcacctt gaacgcgtgg
    18061 agaggcttcg cccggacctc cggcgtcagc tcgcggagcg gcgccgcctc cgcgacgaag
    18121 gcgctcctcc cgtgcgtacc gaggacgacg tcaccctcct tcgggtggac cgcgaggtcg
    18181 tggaccggca cgtgcgggag cccgcccgtc agggcgtacc acgtggcgcc gcggtcgagg
    18241 gagacgtacg cgccgaggtc cgtccccgcg tagaggaggt gcttcgcttt cgggtcctcg
    18301 cggacggtgt tcaccggctc ggccgggagg cccgcggcga tcgaggtcca ggtcgccccg
    18361 aagtcggtcg acttccagag gtacggcgcg aagtcgtcgt tccggtaccc gctctgggag
    18421 acccagaccg tcccctcgtc gtggacggag gcgacgacgc gcgtcaccca gcgctctttc
    18481 gcgaggcctt tcgagaggtc cttccaggtc gccccgccgt cgcgagtccc ccagaccttc
    18541 ccctcgtcgg tcccgacgaa gaggacgccg aacgtcttcg gcgactcgga gatcgtcgtg
    18601 atcgtcccga acggcacgtc gcccggctcg cgcgtcgagg tgaggtcggg gctcacggcg
    18661 gcccacgtct cgccccggtc gaaagagcgg aagagcttct gcgtcccgaa ccagaggata
    18721 ttcggcgagt gcggcgagag gaggaacggc gtcacccagt tgtagcggag cgccggctcg
    18781 ccgagcgcgt ggcgcgggcg gaaccgggtc cgctcgccgg tcttcaggtt cacgcggacg
    18841 gcgttgccga actgcgaggc cgtgtagacg aggtccgggt ccttccggtc gacctcgatc
    18901 caggagccgt ctcctccgat caccggcttc caggcgtcgg gcggggtctt ccccgcggcg
    18961 tacgtcgagg gcccgcgcca gacgccgttg tcctgcatcc cgacgaggac gttgtacggc
    19021 tcggccgagt cgaccgcgac cgtcgtcacc tgtccgaccg ggaggttggc gaccttcgtc
    19081 cagctccgcc cgccgttcca ggagaggttc aagccgccgt cgttcccgag gacgacgtgc
    19141 cgcgggtcgc gcgggtcgaa ccagagcgcg tggtggtcga cgtggacgcc gtgccggtcg
    19201 aggccgttcc agctcctccc gccgtcggag gagcccgcca gcgggacgcc cgccacgtag
    19261 atcttctcgg cgtcgtccgg agccacccag atcttcccga agtagtagcc gtaggtgtag
    19321 gcgaccttct cgagggaggc gtcgtgcgtc ctcctccacg tctctcccgc gtcgtcggat
    19381 cgccagacct ccacgccgac cgtctcgacc tcctccatct gccggtccgc gtcgtcgacg
    19441 taggcgacga cgtcggcgag cttcaccttt ccggaggcga cgtcccgctg gaggacgcgc
    19501 gccttcagct ccttcgggaa gccgttcgcc cggaggaacc gttcgacgac ggccgtctcg
    19561 accttcgcga gctgctcggc ggtcagcgcc ttcagccggc gcggcgtcag ctcgcccggc
    19621 ggcgtctcct cgtccctctc gccggggcgc cgggactggt tgtcgacgac ggcccagacc
    19681 gcgccgggcc ttgccggggt cacggcgagg ccgatccggc cgacgacgtc cccctgcggg
    19741 aggccgccct cgaggcgggt ccaggtcgcc cctccgtcgc gcgagcggaa gatcccgctc
    19801 cccggccccg actcgaggaa gctccacggc ttgcggtcgc gctcccagag cgcggcgaag
    19861 aggagcttcg ggtccttcgg gtcgcggacg acgtcgatcg caccggtccg ccccggcccg
    19921 ccgaggacct tcgtccaggt cgccccgccg tccgtcgtcc ggtagaggcc cctctcgccg
    19981 ccgtcggtgt agagcggtcc catcgaggcg acgaggacgt cgtcgggact cgccgggtcg
    20041 acgaggatct tcccgacgtg gtgggagtcg tgaagaccgg tgttcttcca ggtcttcccg
    20101 ccgtccgtcg acttgaagac tcccgtcccg gcgtaggtcg tccggctgga gttggcctcg
    20161 ccggtcccgg cccagaggac gtctccctgg gcgtctccga cggcgatcgc tccgaggccg
    20221 atcgacgact cggcgtcgaa gagcggcgtc caggtgccgc ccttgtcgtc cgttcgccag
    20281 agcccgcccg tcgcgaaggc gacggagagg gtgtccggac ggcttctcgg ggcgtcgagg
    20341 tcgacgatcc ggcccccttg cagctcgggt ccgacgttcc ggaacgtcag gcccgcgaag
    20401 gggctcgcgc tctccatcgc gaggcgcctc tcgaagcccg cgagacgcgg ggcggccggc
    20461 atcccgtggg caggcgggtg gaagcgcctc gcctcctcgg tcttcgtggc gggaggggcg
    20521 gcgaggagcg gggtggcgag aaggaggggg gcgacgcgcg cgaagctctt catctcgggg
    20581 ctctcggcgg aatcgtacag gggcgacggc accgcggatg ccttcgctgt cgtataaccg
    20641 ggagtgatga agctcgtcac gttcgtccgc cccggcgcgg acccggccgg cgctccgggg
    20701 atcgcgctcc ccggagccgg cgtcctcgac ctcggccgcg cctccgagac gtttcgcggc
    20761 ctctcgatgc tcgacctggt gcggcgccac gaggagctcc tccccgaggc gcgcccgctc
    20821 gccgccggag aagcctccct tcccgccggg accctcgtcc ccgaggacga ggtgcggctc
    20881 ctcgcgccgc tccctcgccc cgtctccgtc cgcgacggct acgccttccg ccagcacgtc
    20941 gagaccgccc ggcgcaaccg cggcctcccg atgatcccgg agttcgacga gttccccgtc
    21001 ttctacttca cgaacgccct cgcggtcatc gggccgggcg agctgcgcgt cgggcgacgt
    21061 cgcctcgaga agctcgactt cgagctggag tgcttcgtcg tcctcgggaa gcgggtcacg
    21121 aacccgacgc tggaggaggc cgacgacgcg atcttcggct acggcgtcat gaacgacctt
    21181 tcggcgcgca ccctccagat ggaggagatg aagctgaacc tcggaccggc caaggggaag
    21241 gacttcgcca cggcgatcgg gccctggctc gtcacgaagg acgagctcgc cgaccggctc
    21301 cgcccctcac cgaaggggaa cgtcctcgac gcgcggatga cggcgcacgt caacggcgtg
    21361 caggtctcgg aggggaacgt cgcgcaaatg gactggacgt tcgcccagat cgtgcaacgc
    21421 gccgcggacg gcgtgacgct cgagccgggc gaggtgatcg ggtcgggcac ggtcggcacc
    21481 ggctgcttcc tcgagctgaa cggctcgggc atcacgaagg accagtggct ccgccccggc
    21541 gacgtcgtca gcctcgcgat cgacggcctc gggacgctga cccacacgat cgtcgaggac
    21601 cccgagaagt gaaggacgac ctcttcgccc tctacgacta caacctctgg gcgaaccggc
    21661 tcctcgcacg ctcgctggcc ccgctctcgc acgaggacta cgtgcgcgag atggggggcg
    21721 ggtggccctc cctccgcgcc acgctcgtcc acctcgccgg cgcgacccgc gcctgggccg
    21781 agcggttcga cgggcgcgac gcgacgtccc ttccggccgt cgaggagctg ccgacgctcg
    21841 ccgacgccct ccggacgctc gacgaggccg acgcccgcat cgcggcattc ctcgcgacgc
    21901 tcggaccgga gcgcctcgcc gccccgctcg tctggaagaa cctgaagggc gagccgaagg
    21961 cggcccctct ctgggccgtc ctccgccacg tcgtgaacca cgcctcgtac caccgcggcc
    22021 aggccgccgc gatgctccgt cggctcggcg ggaacccggc cccgaccgac ctcgtccgct
    22081 gggcgatcga acgccacgag gcgggcgctt tcgaggcctg acggccgtga tcgagacctc
    22141 cttcgagcgc ctcgtgaggc gcgcgatgat ccttgggatc gggaacctcg cgaccccggt
    22201 ctacttccgc tggctgaacg cagtcaaagc cgagggcgcc gagctcctga aggagcttcc
    22261 ccggaagaac gtcgtcttcc tctcgaacca ccagacctac ttcaccgagg cgatcgcctt
    22321 ctacgacctc ctctacatcg tccacggcct cccgtacgag gacccgcacc tgcgtttctc
    22381 cgcggcgacg gagacgatga agaagaacct cctcacgaag gtcctcacct acgcgggggg
    22441 cgtcaccttc aagcgctcct tccgggaagg cggccgggag gtcaaccgac ccgtcgacct
    22501 cgagggcgtc tcgaaggtcg aggaagcgat cgccaccggc tggctcctcc acttcccgac
    22561 cgggacgacc cagaagaacg cgccgatccg cccgggcatg gcccagctcc tccaccggac
    22621 gaagccggtc gccgtgccga tgcgggtcga cggcttccgc gaactgctgc tccacaagca
    22681 gttccccggg aggacgggca agaaggcgac gatccgcctc ttcccgccaa tggacctgac
    22741 ggagttctac gggcagccgt tcgacaagga gcgcggccgc aaggtcatcg aggagctcgg
    22801 gaagatcatc ctcgacccgg cgtaggccac ggctcaccgg ccgcggagcg ctccgccgca
    22861 cgccgcgcct ggaacgcgcg gaagttctcg tcggcccagg ccgcggactg cgcgttcagc
    22921 gaggccaggt cgcggtgccg gtagacgccg ggcgggaacc gaagcttggc cgtcttccgt
    22981 ccgaagtccc agagcccctc gatgacgcga gggagccggg gaccggtccg ctcgtagccg
    23041 cgcgccgcct tcatctcctc gatggaccgg aacttccgga cgggcatcag ggctcctcga
    23101 atccgtactt cgcccgaagg ccgtcggcgt cgagccggtc cttggggcgg acggtgtcct
    23161 tcttcatccg gaagagagtc cggggcgaga ccagccggac cttcacgcca tcgagcgtcg
    23221 tctcgtccgg ctcgaggtcc tcgaaccgaa atgcctctcc caggcgcgtg aggatgtcga
    23281 cgtggaagtc cccttcgggc gagttcatcg cctctacccc ttcgcctccg gcttcgcctt
    23341 cagcgaccgc gcgacgagcc cgacgcctcc ggcgaggagg ccgagcatga tcagcgtgtt
    23401 gtagaggctc gtcggccgga accagatctg caggttcgcg acgatgccgg cgacgatgat
    23461 gagcgccccg gctcccgtca gcagccagcc gacgaccgac ttcccgtcga agaagaggaa
    23521 gccgatgccg acgaggagcg ggaccagcgt caggccgaag gtgttgtgcc cgaaccagtc
    23581 ccagaaaccg ctgtggacct ggacctgctg gaggaggagg tagcccccga cgaccatcat
    23641 tgcgagaccg ccgaggaagg tcccgacgcc gcccggcgtc ccgcccgcgc ctttgatctc
    23701 catcaccgaa tcatacgaaa ccgccgcgaa ggcccgaggc cccctcctct tcgaagctaa
    23761 gatcgctgca ctgcggcagg agggttcgag atgaagagat gctccgtcgg cctcatgggt
    23821 accacgttct ccgtcctcgt cctcgcggcg tcccccgcgg aggcgcagaa cctcgtccag
    23881 aacggcgggt tcgaccggac gatctcgtcc tggtcgagct cgacctccga gatctcgtgg
    23941 agctcggatg actggaaccg ccagcccggc tcgggatcgc tctcgctgcg tcacaccggg
    24001 acgaccgcga acggatacgc gagcgtcggg cagtgcctcc cgtcccagcc cggcaagggc
    24061 tactccttcg tcctgcgcgc ccggatcgtc gaggcgaccg gcggcgcccg aggcggagtc
    24121 gaggccggcg tccacttctt ctccgggacg gactgccggg gctggctcgc cggaagcgat
    24181 aaccgctggg tcgagctctc ctcctcgtgg acgggggtcg cgtacccgtt cctgatcttc
    24241 ccgtcggccg tgtccgtcgc tccggccggg acccgctccg tcctcgtcaa cgtgaacgcc
    24301 gtcaagctca cggccgccgg cgcgctcacg ttccgcatcg acgacgccta cttcgccgaa
    24361 gccaagccga ccgtcctgac gatcccggcc tcggcctcgg cccgagggga aaacgacacc
    24421 ttcttccaga ccgacctctg gctcggcgtc gcctcgagcc gcgcggacgt cccggtcgcc
    24481 gttctcggag agcaccgctg cttcccggga cagacgtgca cggggcggat gcactactgg
    24541 accgacgtcc tcccccgcag cttcacccgg accgtcgaca cggtcgggac cctcttcaag
    24601 gacccgtcga cctccggcgc catcgagctg acgtacgacg cctcgatcag cacgatcgcc
    24661 gcgatgtcgc ggacctactc gccttcgctc cccgcgccga cgacggggac cgcgattccc
    24721 gcgctgcccg cttccgaagc gcggacgcgc tcgctcctga tcggcctgag cgcttcgggc
    24781 ggggaccgat cgagcggctt ccggaccaac gtcggcgcct acaacccggg caatgcctcg
    24841 gcggaggtga ccgtgcggct gtacgacgcg gccgggcgga cgatcgggtc cccgatcgag
    24901 cggaccgtcg gcccgcgcga gccgatgcag atcaacgagg tcttcggcga ggccggcgtc
    24961 ggccaccttc tcacgaacga ggcgcacgcg gtcgtcacct cgacgcggcc ggtcttcgcc
    25021 ttcgcgacgg tcatcgacaa caggtccgcc gactcgatct acgttcccgg ggctcccgac
    25081 gcgccctgac ggggcgagcc ggggtcggcc cgttcagccc atcgcccgga ggtgctcgac
    25141 gaccgcctcg acgtcgtagt ccggagtcga ggcgcccgcc gtcacgccga ccttccgcgc
    25201 cccctcgaag tcctccgggc ggaggtcggc ggcggtcccg acgaagacgg tccgcttcgc
    25261 cccttccgcg atcttcacga gctccttcgt gttcttcgaa tggcggcctc cgatcacgac
    25321 gatcgcgtcg atcgacgggt cggcgcagag cgcccggagg gcgtcctggt tctccttcgt
    25381 cgcgtagcag atcgtgtccg cgacccggag gtcggggacg cgccgcctca tctcggcgac
    25441 gtgctccgcg aacccctccg cgttcagcgt cgtctggaag acgaccttca ccttcgggtg
    25501 ctcctcccac gggaacgcga cgacctcctc gagcgtgtag aagacgccgt agacggacgg
    25561 gtcgaggtcc ttcgtgtagc cgatcacctc gcggtgcccc tgctgcccga gcacggcgat
    25621 gaagcagccg tcgtcgaggg agcggccgat ctccctgtgg atgtccgtca cgaacttgca
    25681 cgtcgtgtcg atggccgaca ggcccagcgc agcggcctcg tcgcggaccg agggcgcgac
    25741 gccgtgggcc gagaagacga tccggccgga gtgcgcgtgc gcttcctcga ggcggtggac
    25801 cgtcgggaag ccgaggctcg ccatctcctt ctcgacgctc tcgttgtgga cgacctgccc
    25861 gaggatcgcc cccgactgcc ccttcccgac cgcgatccgg acgagcttgt cggcgacgcg
    25921 gaccccggcg cagaagccgt acttctcggc gaggacgacc tccatcgtca cttccctccg
    25981 ttcgtcgggc ggtccggaag gtcgaggagg acgatctcgg gagggacgac cttcacgaac
    26041 cggtcccgct tgatcatctc gaaccccttc ccgccgcgcc cgacgacgtc gtaccggcgc
    26101 gtgaccgtgt acggcgtccc cttcgagttc tcgagggtca ggaccgcgct ccgcttctcg
    26161 tccctcccga agagcgccgc gccgacgacc cggtcgtcgt cggcgaggcg gatgccgtag
    26221 acccccttcc ccggccccga gaggacgggg acctcgtccg agtcgaagag aatcgcccgg
    26281 cccttctcgg acgcgagcgc cacgatcgcc cccggctcgc agacgccgac gaggaggacc
    26341 tcgtcgccgt cgccgggctt cgcgaaacgc ctcccggcgc gcgtcgtcgc ctcccggagc
    26401 ggttcggccg agaagcggag gaccatcccg tcccgggtcg ccgcgaggag gacgacgtct
    26461 tcgggggggg tcacgcgcgg gtcgagcgaa agggccgcga cgaccttctc gccgtccgcg
    26521 aacttgaaga gtttctgcac cggctcgcca tagcccgtcg agggagggac gtcgttcatc
    26581 ttcaggacgt aggccgaccc gcggctcgag aagaggacga cgagctcgcg cgtgccgccc
    26641 tggaggacgg cgaggacgtc gtcgccctcc ctcaggcgcg ttcccttcgg gtccttcagc
    26701 tggccgagcc gcttgaccca cccgtcgacc gtgacgaccg cgaaggcgtc ctcgtcgggg
    26761 ataaaggcct ccgcgtcgta ctccggctcc tcgaccgcgg ccgagacgcg cgtcctcctt
    26821 ttctcgcccg agagaacgcc gcgcagctcg acgagctcgc gctggacgac cccccagagc
    26881 ctcgcggggg acctcaggat cgcctcgatc cgtgccgcct cggcccgctt ctcggcaagc
    26941 tccttccgga tcgcctcgat ctcgagcttt gcgagcttgt agagcttcgt ctcgaggatc
    27001 gcgtcggcct gatcctcgtc gaggccgaag cgcttcatca gcttcgccgc cgcgtccgcc
    27061 ttcccgtcgc tccgccggat gatccggatc gcctcgtcga gctcgtcgaa gatcttctcg
    27121 aacccctcga ggaggtgaat tcgcttcttc agctcctcga gctcgtactc gaagcgcctc
    27181 ttcaccgtcg cgaagcggaa gtcgaggaag tgccggagga tcgccgcgag cgggagccgc
    27241 gccggctcgc agagctccgg gttctccgtc gggacgagcg cagtcaggtt caccgggacc
    27301 gtcgtctgga gcggcgtgtg cttgtagagg tagctcatga cgagcccggc gtcggcgtcg
    27361 cgcttgagct cgaggacgat ccgcacgtcg tccgtcgact cgtctctcac gtcgacgagc
    27421 gcggggagct tgcgcgcgat gatcacctcg gcgatcttct cgacgagcga ggccttggcg
    27481 acgccgtacg ggaccgacgt gacgacgatc gcctgcgcgc cccggccccg ctcctcgacc
    27541 tcgtactcgg cccggagctt gagcgtcccc tgccccgact cgtagacctc gcgcagctcc
    27601 ttcttcgtcg agaggagctg gccgccggtc gggaagtccg ggcccttgat gaacctcagc
    27661 gggtcggccg ccgggtcgtc gatggcggcg atcgccgcgt cgaggacctc gcacgggtta
    27721 tgcggcggga tcgaggtcgc catgccgacg gcgatgccgg tcgcgccgtt gacgaggagg
    27781 ttcgggaagc gggccggcag gacgaccggc tcgaagtgga ccccgtcgaa gttcggcttc
    27841 cagtcgacgg tccgcttccg gatctctgcg aggagctcga ccgcgagcgg gcggaggcgc
    27901 gcctcggtgt accggtaggc cgcggcggag tcgccgtcga gcgagccgaa gttcccgtgc
    27961 ccctcgacga gcggggcccg gagcgagaac ggctgcgcca tcctcaccat ggcgtcgtag
    28021 atcgcgacgt cgccgtgcgg gtggtacttg ccgatgacgt cgcccacgac cgacgccgat
    28081 ttcttgaact tggcgtcggg cgtcaggtgg aggttctggt acatcgcgaa gaggattcgc
    28141 cgctggaccg gcttcaggcc gtccctcacg tcggggaggg cccgcgccgt gatgaccgag
    28201 agggcgtagt tcaggtagcg ccggcgcgcc tcctcgggga gggcgatctc ggcgtcgaag
    28261 gcgagcgccg gctggccggc gccgtcggag cgtttccgcg tcatgagtgc ctcaaaagag
    28321 aatccccccg gccctcgggg ccggggggac ggatgttacg gtctgccgcg gccccggtgg
    28381 ggccgccccc tcaccgcgcc gacttggccg gcttgttcgg cgagctgagc gccgccatca
    28441 gggaagctcc gaagttcgtc gggcgccccg gctcgctcct ctcgcgcttc gcggagtcct
    28501 cgcggtactt ccggatctcg ccccgctcgg cctcctcgcg ggcaccggtc gcggaaaggg
    28561 agatgcggcg gcgctgggcg tcgaggccga tgaccttgac ctcgaccttc tggccgggag
    28621 cgaacgtctt cgaggcgggg gtcccgtgcg ggagacccgt ctcggagttc gggatgaggc
    28681 cggtgaggcc gatctcgagg cggacgaaga cgccgaacgg cgcgacgttc tccacctggc
    28741 ccgcgacgac cgagccctcg ggcatcttct cggcgagccc gtcccacggg tcggtcgcgg
    28801 aggcctcccg cagggagagg gagagccgcc ggtgctcctc gtcgatctcg cggacccagc
    28861 cggagacctc ctggccgacc gcgacggacg ggtccttcag gtcgatgccg ggcgggagct
    28921 gggagacgtg gacgaggccg tcgatgccgg gctcgatctc gatgaagagg ccgaactcgg
    28981 cgtggcgcgc cacgggcttc gtgaaggccg tcccgggcgg gatcctctgg gcgaggccct
    29041 cccacgggtc cttctcgagc tccttcatgg agagcgagat ccgcttccct tcgttctcca
    29101 ccttcgtgac cttgacctgg acctcctggc cgatcgtcag gacgtccttc gcgtgggcca
    29161 cgcggcgacg ggagatctcg gtgacgtgga cgagcccgtc gaccccgccg aggtcgacga
    29221 aggcgccgaa gtcggtgatc gagcggacct ggcccgtcag gacggccccg gggacgatcc
    29281 gctcccggat ctcgccgagg ctcttctcga gcgcctcgcg caggatcgcg gcccggctga
    29341 cgacgacgcg ccggccgtcc tcggcgtact cgatgacgcg gaagtcgaac gactgcccga
    29401 cgaactgcag caggccgggc tcgtcgtgac ggccgacgtc gatctgggag accgggcaga
    29461 acgcgcgcgg tccgcccgtt ccgccgatgg ccacctcgaa cccgcccttg ttgcgggaga
    29521 ggaccttgcc gaggaccggg atcccggtct cccacgcggc acgcagctcc gccttcgcgc
    29581 ggcgcgcgac ggcgagcttc ctcgagagcc ggatgtcggg cccggccttg atgaccacgg
    29641 cgtcgatccg gtcgccgacc ttctcgccct ccagctcgtg gaggtccatc aacgcctcgg
    29701 acttggcccc gatcgagacg aggaccacgt ccggcgtgat gccggcgatc accccgctga
    29761 cggcctcacc ttcctgtggc cgcttgctgg cgggaaaaga cgcctcgagg gcggcttcga
    29821 atgcgtgacg atccgccgac tcggtggagt cgacgggggg agcggcgacg gcgtctgtgg
    29881 tcacggacgg aactcctggc ccgcgggact ggaatcgccg ggcggcccgg cgaggcgcgg
    29941 gggatggatt gtactcctat cggccagcgg aaggggcggc gacgatgcct ccccccggtt
    30001 cgtccccgga aagagaaccc cccgcgtcct ctttctcgta ttatcggggg tttcgtggct
    30061 cgagaagagc ccggccggtc gcggacgccc ggccatggag gacacccttg accgaatcgc
    30121 tcctcgccgc cccggagacc gccgccgccc cctccgccgt cgccccgacg cgggaggagc
    30181 ttcgcaggtg gtactccctc atgcacctgg ggcgggtcct cgacgacaag gcgccgaact
    30241 acctcaagca gggcctcggc tggtcgtacc acgccccgtg cgccgggcac gacggcatcc
    30301 aggtcgccct gggaacggtc ttccgcccca cgttcgacta cctcttcccg tactaccggg
    30361 acctgacgac ctgcctcgcc gcgggcgtca ctcccgagca gatcctgctg aacgggatgt
    30421 cgaaggcgac cgacgtctcc tcgggcggcc ggcacatgtc gaaccacttc gcccggcacg
    30481 acctctacgt ctggaacgtc tcctcctgcg tctcgaacca cgcgcagcac gccgccggcc
    30541 tcggccgcgc gatccgctcc tacggacttt cgagcctggt ctactgctcg atgggcgagt
    30601 cctcgacctc ggaagggtat ttctacgagg ccgtcaacgg ctcctcgctc gggaagctcc
    30661 cggtcatctt cgtcatccag gacaacggct acggcatctc ggtcccgaag cgccagcaga
    30721 cggcgaacca ctacgcctcg gacaacttcg cctccttccc gaacctgaag gtcgtccact
    30781 gcgacggcac cgacgtcctc gattccgtcc gcgcgctccg ggaggccgcg gcgttcgcgc
    30841 agagcggcgc cggctgcgtc ctcgtgcacg ccgactgcgt ccggatcggc tcgcactcga
    30901 actccgaccg tcacgagctc taccgcgacg acgaggagct cgccgccgcg cgcgagcggg
    30961 accccctccc gcgcttccgc cgctacctcc tggagtccgg cggcttctcc gaggaggagc
    31021 tccgcgccct cgaggacgag aacgcgagaa cctacgaggc cgcggccgac cgcgcccgga
    31081 gcgcgcccga ccccgacccc cgctcgatcc acgacttcgt cctcccggag ccgtgggtgc
    31141 cggacgggga cgggctcgcc ggcgtcgccc ggaccccgcc cgcctcggcc gacggccgcg
    31201 cgaaccgcga gtcgaccctg atccacgcga tcaacgagac gatgaaggac ctcttccggg
    31261 agaactccga cacgttcatc tggggccagg acgtcgcctc caaggagaag ggcggcatct
    31321 tcaacgtcac gaaggggatg gagaaggagt tcgggagcca ccgggtcttc aacgccccga
    31381 tcgccgagga ctacatcctc ggcatggccg acggcttctc gcggatctgc gacggcgtgc
    31441 gggtcatcgt cgagggggcc gagttcgccg actacttctg gcccgccgcc gagcagatgg
    31501 tcgagatgtc tcacgagtac tggaggacga acggccagtt cgtcccgaac gtcaccgtcc
    31561 gcctcgcgtc gggcggctac atcggcggcg gcctctacca ctcgcagaac gtcgagggct
    31621 ggctcacgac gctccccggc atccgcatcg tcgtcccggc gttcgccgac gacgccgcgg
    31681 gcctgctccg gaccgccgtc cgctcccgcg gcatcacgct ctacctcgag ccgaagttcc
    31741 tctacaactc tccgctcgcg aaggccttcg tccccgacgg cttcgccgtc ccgttcggga
    31801 aggcccgcct ccggcggccc ggcacggacc tgacgatcgt ggcctacggg acgccggtcc
    31861 actgggcgct cgaggccgcc gggacgctcg cgaaggaagg gaaggaggtc gaggtgatcg
    31921 acctgcgctc cctcgccccc ctcgacttcg acgcgatcgc cgattccgtc cggaagacgt
    31981 cgcgtctcgt gatcgcccac gaggacaagg tccagggcgg attcggcggc gagatcgccg
    32041 cgcgggtcac gtccgagctg ttcgagtacc tcgacgctcc cgtcgagcgg gtcggctcga
    32101 cgttcacgcc ggtcggcttc aaccggatcc tcgagcgggc gatcctcccc gactcggctc
    32161 gcgtgctttc ggcggcgcga aaggtcctcg cctactgacc gtggggcggt ccgcccctcc
    32221 ggggccgggc cgctctatca tcttcggatg catccggggc caaacggctc ctcgaaaggg
    32281 acgcccgaaa ggacgcggcc gacgcgggcg cttcgcctcg tcgccccggt cctcgcgctc
    32341 gcgctccttc acgtcgcgcc cccggcccgt gcgctcgacc ccgcgcggtc tctcgcccag
    32401 tcgacgctcg acacgtggga gacgggccag ggcctccccc agaacacggt ccaggcgctc
    32461 gcccagacgc cggagggcta cctctggatc gggacccagg aggggctcgt ccgcttcgac
    32521 gggacgcagt tcaccctgtt cgaccaccga accaacgccg agctttcggt cagctccgtc
    32581 ttcgcgctcc tggccgacct cgacggagcg ctctgggtcg ggacgaacgg aggcggcctc
    32641 ctccggatga cgggtagccg gttcgagacc ttcggcccgg agacgggcct gacggggctg
    32701 aaggtccagg ccctcgctcg cgacggccgg ggtgtcctct gggtcgggac cgtcgaccgc
    32761 gggctcttcc ggcgcgtcgg cgggcgcttc gagaaggtcc gggtcggcgc cggcccgcac
    32821 gagctgccgg tccgtacgct ctgcgtcgac cgctccggcg ccgtctgggc cgggacgatg
    32881 gggagcggcg tctcccgcct cgacccgggc ggaccggtgt cgttcacgac ccgggagggc
    32941 ctcccgaacg acaccgtcat cgccctcgcc gcgcaccctg cgggagggat ctgggtcggc
    33001 acggccggcg gcggcctggc acggatcgcc gacgggaggg tcgccccggg cggcgcgccc
    33061 gcgctcccga cgggccaggt ctccgccctc ctcgtcgacg acgccggcaa cctctgggcc
    33121 gggacgtggg gccgcggcgt ctgtctcgtc tcgggagaga acgtggcctg ccagtccgcc
    33181 cggacgggtg agctcccgtc ggaccacgtc tggagcctct tccaggaccg ggagcggagc
    33241 ctctggatcg gcacctggac agacggcctc ctccgcctgc ggcacggggt cttcgcgagc
    33301 tacggacgtc gcgaggggct cgcgggggag aacgtccgtg ccgtcgccga ggacgcgtcc
    33361 gggacgatct gggcgacggt ggcgagcggc ggcctccagc ggatcaccgg gacctcgatc
    33421 cgcgtcttcg ggccgaagga cggcctcccg tcggaccagg cctcctccat cctcccggcc
    33481 cgggacggca ggctctgggt cggcacctac accgacggac tcgccgtcgg ccgggacggc
    33541 gtcttccggc gggtcgccct gccgccgccg gagtgggcgg cgcgcgacgt gcgtgccctc
    33601 gtcgaggacg aggacggctc gatctgggtc gggctcctcc cgggcggcgt cgtgcgcctg
    33661 cgcggcggtg aggcccggcg cttcggcgtc gccgatggcc tcccttccga tcggcttctc
    33721 tgcctccatc cggcccgcgg ggggggtgtc tgggccggaa cgacggcggg gctcgcccgg
    33781 ctcgagggcg accggttccg gtccttcacg aagcaggacg gcctcccgga cgaacggatc
    33841 caggccctcg ctctcgacga cgaggggacg ctctggatcg gtaccgggaa cggcctggca
    33901 cgcctgcgcg gcggcacgat cgccgcggtg ggaacggccg agggactctt cgactccgtc
    33961 atcaagagcg tcgtcctgca cgaaggctgg ctcttcatga cgtcgaacca cggcgtcttc
    34021 cgcgtccgac gcgccgaggt ctcggcggtg atggacggcc agaggcggag gttcgcgtcg
    34081 gagcacttcg gcctcgccga cggaatgagg agcgaagggt gctccggcgg cacgcttccc
    34141 ggcgcggtcc gcgcctcgga cggccgcatc tggatcgcca ccacgcgcgg cctcgccgtc
    34201 ctcgacccgg agcggctcga gcggccgacc gcgctcctgt cggtccggat cgagggggtc
    34261 cgcgcggacg gtgcaccgat agagccgggc gagacggtcg tcgtgcctcc cggctcctcc
    34321 cgcctcgaga tccgctacac cgggatcagc ctcgtcgcgc cggcggacgt ccgctttcgc
    34381 tttcggctcg acggcgtcga cccggcctgg gtcgacgccg gtcggggacg ctccgcgagc
    34441 tacaccaaca tcacgccggg cagctacgtc ttcgaggtcg ccgcgcggcg cggcgcgggc
    34501 ccgtggacga ccggggcgaa gacgacggtg cagatcgagc cgaccctcgc ccagaaggcc
    34561 ttgttccgcg cggccgtcgc cgggggcgcc gtcgccctgc tcgccttcgc gctcttccgg
    34621 cggggccgcg agcagcggca gcggcgcgcc cgcctcgagg agctggtcgc tgcccggacg
    34681 gcggagctcg agaccgccac ccgcgaggcc gaggacgcga accgggccaa gagcgccttc
    34741 ctcgcgacga tgagccacga gatccggacc ccgatgaacg gcgtcatcgg gatgacgagc
    34801 ctcctcctcg gcacgccgct gacccgcgag cagcggcagt tcgccgagac gatccggcag
    34861 agcggagaga cgctcctcca cctgatcgac gacatcctcg acttctcgaa gatcgaggca
    34921 ggccggatgg gcctcgagag gcgccccttc gaggtgaggc cgtgcttcga gtcggcggtc
    34981 gacctcctcg ccccccgcgc ggaggagaag cgcctcgaga tgacgctcga cgtcgacgac
    35041 ggcgtcccgg tcgcgctcct gggcgacgtc acgcgcatca agcaggtggt catgaacctc
    35101 gtcgggaacg ccgtgaagtt caccgacgcg ggcgaggtcg cggtgacggt cgtatcccgc
    35161 cccgcgggca gcccgccccc ggggacgctc gtcgagatcg aggtcgcggt gagggacacc
    35221 ggcatcggca tccgtccgga gcgggccgcg agcctcttcg agtcgttccg ccagggagac
    35281 agctccacga cccggcgctt cggcggcacc gggctcggcc tcgcgatctc gcgacgcctg
    35341 gccgagctga tggggggctc gatctcgttc gagagcgcgc cggggcacgg ctccacgttc
    35401 cggttcgtcg cccgcctcga ggctgcgatg cccccctcgg gggccatcgt cccgtcccgg
    35461 cgcgcgagct tcgccgggcg gcgcgtcctc gtcgtcgagg acggcgcgac gagccgcgcg
    35521 atcctcgcga aggtcctctc ggggaggggc ctcgacgtga cggcctgccc ttcggtcgcg
    35581 gccgccgcgg aacgactccg ctccggggag cgcttcgacg gggccgtcgt cgaccgggac
    35641 ctgcccgagg gcgacggcct cgccgccttc acggccgcac ggtcccgcca cgcgccttcc
    35701 ctccccttcg tcctcctcgt cccgatgggc gccgagaccg aggatggggc ggacgggccg
    35761 gccgcacggg tgacgaagcc ggtcaagccc gccgccctcg tcgaggccgt cgccgccctc
    35821 gtcggcggcg gccgcctcgc gcagaagcgg gagtccgcga tccgcccgcg tttcgacggc
    35881 tcgatgggcc gccggctccc tctccggata ctcctcgtcg aggacaatcc gacgaaccag
    35941 gagctcgcgc tcctcatgct gggccggctc ggctactcgg cggacgtggc cacgaacggc
    36001 cgggaagcgg tcgaggcggt cgccgcccgg gtcttcgacc tcgtcctgat ggacattcag
    36061 atgccggagc tggacggcat cgaggcgacc cgccagatcc gtgccgcgct cggagccgac
    36121 ggcccccgga tcgcggcgat gaccgcccat gcccttccgg gagaccggga ggcgtgcctc
    36181 gccgcgggga tgaacgacta cctcgtcaag cccatcggaa tctccgacct cgtcgcggct
    36241 ctcgagcgcg ccgcgaccgc gctctcgccc ctcgcggagg gcgacgacga cgcgaccgtc
    36301 gcgccggcgc ccctcccgcc tccgccgctc gcgacgcctc ccggcctcga cggcaaggcc
    36361 tgggcccgcc tgcacgcctc cctcggcgcc cgggccggcg agctccttcc gggcatcgtc
    36421 cgttccttcg cgcgcgagtc ggacgggctc ctcgcctccg cccgcgccgc cgccgcggcc
    36481 gagcgggcgg acgagctcca ccgggccgtc cacaccctcc gttcgacgag cgcgagcttc
    36541 ggggcgctcg acctctccgc gctctgccgc gaagccgagg cccgggcgaa gcagaacgag
    36601 acgacgacgc tcgagcctct cctcgaccgg atcggccacg agctcgtgca ggtgagaaag
    36661 gtgctgggcg ccatcccggg agacggtccg atggacgagc ccgtcccctc cccgaagtcc
    36721 acggactgag ccgcggggcc ctcagctcct cggcgggagc gtgaagcgga ccgtcgcgcc
    36781 ggcccccggg gcgccgtcga tgcgaatcca gccgccgtgg cggcggacgg cgctctcggc
    36841 gagcgccagg cccactccga ggccgggcag ctccgactcc gcgtgagctc gctcgaacag
    36901 cccgaagaga cggccggcgt gagacggatc gaagccgacg ccgtcgtccc gcacgaagac
    36961 gaccgtctcg ccgcgctccg tctccgaccc gacctcgatc ctcgcgtgct cccgcggcga
    37021 ggtgaacttc caggcgttcg agagcacgtg ctcgaggacc tcgccgacga ggcgacggtc
    37081 cgcgcggagg acgagccccg acggcgtgct ccacgcgacg cgcctctccg gctcctccgc
    37141 cgcgtgccgg cgcaggatcg cggccgcgag cgcgccgacg tcgacggcct cgcgctcgag
    37201 cttcgcgacc gatatgcggg cgtggtggag cagcgcctcg atcagcttgc cgccgcgcgc
    37261 gctcgcggcg ccgatccggc ggagaagccc cagggcctcc tcgtccagtc tcgccccgta
    37321 gtcctcgagg agccggccgg cgaggccgtt gatcccgcgg aggggggccg tcaggtcgtg
    37381 cgcgacggtg ctcgaaaagc tccgcagctc ctcgttcgcg gaggcgagcg ccgccgtccg
    37441 ctcttcgacg cgccgttcca gctcggcatt cagcgctcgg acgagcgcct cgctcctccg
    37501 gagggcctct tccgtccgcc tccgctcggt gacgtcgcgg atgatcgtcg ccgtccgcgc
    37561 ccggccccgg gcgtccgtga agaggtccga ggtgacctcc gccgggaagg tctcgccgtt
    37621 cttccggagc ccgcggagct cgccgcggaa ccgaccggtc cgggcgcgtt cctcgagggc
    37681 cgccgcgagc cgcgggtcgg acgcgtcgag cacccccgag cgcccgagct gcaggatctc
    37741 ttccctcgtg cgcccgagca tgcggcaggc ggcgggattc gcggccgaga ccgggccttc
    37801 cgggagggtc aggaagaccg cctcgccgac gttctcgaag atcgagcgga gcgtctccct
    37861 ctcctcctcg agcgccgcct gcgcgttccg tcgggacgtg acgtccgcga agaagatcag
    37921 gtcgcagacc tccccgagcc aggtgacccg gtttccggtc acctccaccg cgaggctcgt
    37981 cccgtccgca cggagaaaga gcgcctcata ggtccgaggg ggggattccc cggtgaggcg
    38041 ttctgccagc cgctgtcgga ggagatcgcg gcaggacggg tcggcgatcg cttcgaaccg
    38101 gctcgccagt aggccgtcga gcgtcgtgcc gagcgcttcc accgcgctcg cgttcgccca
    38161 gcggaaccgc ccgtcccggc cgaccacgag gatcccgaac ggggcgagct cggccaggca
    38221 acggaacccg gggtcggtat cctccggacg gagcgagccg gagtccggcc ggctctcgct
    38281 ggcggccttc gactgtgagc cgggagtccg agtcccgctc gccggcgcca gggcgctctc
    38341 catggctgca gccgcgattc tagctcccgc ccgagcgcct acctcgggct cggagcctcc
    38401 gccacgatcc gcgagaggag cgacgggtcg tccgggagga ggcccgcctt cgtcagccgc
    38461 gggatcatct ccgcccagct ccggtcgatc gcgaagacct tccggaagag cggcagcgcc
    38521 tcgtcgacct ttcccatccc gacgagggag accgccgtcc agtaggtcat ctcggcgttc
    38581 ccggggacga tcgcctgggc ggcggagtag gccttcagcg ccgccgcgtc gtccttcctc
    38641 tcgacggcga ggtctccctc gttcatcagg gcataggccc gcgcgagcgt cacgagccgg
    38701 cggagctccg ggagcggcgc cgggctgtcc tccacgcgga ggtcgaagag ccggtcctgc
    38761 caggggcgcc ccgtcggagc gccggagacg acgatgagcg cggcggactg cttcccgcga
    38821 atgtcgcccc ccgcggcctc ggccgcctcg agcgccgcga gcatccgctc ggcgaggtct
    38881 cccttcgtcc cctcgaacgc cttcgccatc gcgggccaga ccgaggcgtt ccgcatcatg
    38941 ttcgcctgga cggagaagtc cttcccgacg tggtgccccg ccgcctcgac gcagcgcgcc
    39001 cccgtgtggg ccgcgacgcg ccccgcggcg tcgaccatcg cgacctgcct cacgtcgcgc
    39061 cccgcgtcgg ccgcgaggag gcccgcgagg gcgtcgggcg cgcctcgccc tgccttcagg
    39121 agcgagaggc cgagcgggcc gtagctcgcg tcgacgaacg actgcgtcgc gactgcgccg
    39181 acgccggctt ccgcccacgg gacgagcgcc ccgacggaga accagtgcga ctggaccgcg
    39241 acgccgagct gccccgtcgc cgggtcgcgg gccacgatcg agtaggtgtg gacggggcgc
    39301 gcggggaccg ggatcgaggc cgggaggtcc ggcacgccga gctgcgtcag gacgaacgcc
    39361 tggtcgaacg cggtctcctt cagccggtcg taccgccccg aggagccgcc gtgcccgccc
    39421 gccatgttcg tcttcaggag gagcgggttc gcgtcggtct tcagcgtccg gagcttcgcg
    39481 acccacttcg ccggctccca gtacatgacc tggctgtcgt tgaaggaggt cttcacgagg
    39541 accgaggggt acgccccctt cttcaggttg tcgtaggggc tgtacgagcg catgtaggcg
    39601 tactcgtcct tcttcttcgg gttcccccac tcctcgaact cgccgaccgt cagaggcagc
    39661 gtctcgtcga gcatcgtgtt gatgacgtcg acgaacggga cctggaggat cgcggcccgg
    39721 aagaggtcgg ggcggaggtt caggacggcg ccgatcagga ggccgcccgc gctgccgccc
    39781 tggatcgcga tgcggtcctt cgaggcgacc ttcgccgcga cgagcgcctc ggcgcacgcg
    39841 acgaagtcgg tgaacgagct cttcttggaa agcatccggc ccgcttcgtg ccacttcttg
    39901 ccgaggtcgc cgccgccgcg gacgtgggcg acggcgaaga cgacgccgcg gtcgaggagg
    39961 ctcgggagcg ccgggctcca cgaggcgaac gacgggtagc cgtaggcacc gtagcccgtg
    40021 aggaagagcg gggccgtgcc gtcgcgcggg aggtccttcc ggaagacgac gcttaccggg
    40081 accttcgtcc cgtcgggggc cgtggcgaag atccgctccg accggtagcg cgccgggtcg
    40141 tagccgcccg ggacctcggt cttcttcagg accgtccgct cccgcgtcgc gaggtcccag
    40201 gcgaagacga tcggggccgt cgtgaaggac tggaacgtga agcggaacga cttcgtgtcg
    40261 tactcggggt tcgcctcggg aaagaccgat gcgatcgcct cggggagctc gatcgggtgc
    40321 gtctcgcccg tcgcgaggtc gcggaccgcg agtcgcggct gcgcgtcctg ccgctcggag
    40381 aggacgaggt gcccccggaa ggcctcgact cccgagacca tcacgccgtc ccggcacggg
    40441 atgagctcct tccacgaggc cttaccgggg ctcgcgaccg gcgccgtcac gacgcggaag
    40501 tcggggcagc cgtcgttcgt ccggatgtag aggacgtccc ccgcagcctc cacgtcgtac
    40561 tcgtggtccg gctcgcgagc ctggacgacg cggggcgccg cctccggttc cgcggcggga
    40621 aggagcctcc actcgctctg cgtgtggctg tcggagcaga ggaggatcca ccggtcgtcg
    40681 cgcgagcggg agacctcgag cgtgaagcgc tcgtccttct cctcgtagac gagcgcgtcg
    40741 ggccccgtcg tcccgacgac gtgccggtag aggcggtgag gccgcttcgc ggagtcctcg
    40801 acgacgtaga agagcgtctt gccgtcggcc gcccaggcga cggagtcgac cctctcgacc
    40861 cggtccgaga ggagcttgcc ggtcgtcagg tccttcacga agagggtgta gaggcggaag
    40921 ccggtcgtgt cgatcgaata ggcgaggagc ttcccgtcgt cgttcggctc gaagaggccc
    40981 cgcccgacga acgcctcgct cttcgcgacc tcgttcaggt cgaggacgac ctcctccttc
    41041 gcctcgaggc tccccggctt ccggcacgag atcgggtact ggagcccctt ctccgtccgc
    41101 gcgtaccacc agtacccgcg gtgccggtac gggaccgaga ggtccgtctc ctggacccgg
    41161 ccgacggcct cctcgtagag cttcctgcgg agcgcttccg acggcgccgt gacggcgtcc
    41221 gcgtacgcgt tctcggcgtc gaggtacgcc ttcacctcgg gagcctgctt ctccctcagc
    41281 caggcgtagt cgtccacgat cttctcgccg tggatctcga tgaccttcgg gaccttcctg
    41341 gcgacaggcg gcttcaccgc gggagtttcc cctgccgaag ccaggaacgg aacgaggagg
    41401 agggagagaa cgatcggccg cgtcgtacgc acccgaacag gatagagccg agcgcggggt
    41461 caggtcttgt aatgtgactt tcgcgaggcc gcgaaagtca cattacaaga cctgacccca
    41521 gagtccccag agtcgggtcc tccccctcgg gtctcacttc gtcgtggcgt gggctccctt
    41581 cgcgcgggcc ttctcgcgct cggccttcgc ggcggcgagg gcctgaatcg tcgcgccgtc
    41641 gagcgtgccg gagatcgtca cggcgttccg gtccgaggcg accttgaact tccggatcgc
    41701 cgagacgacg tcgggcttct cctgtgcccc gatccgcacg gccgcgagga ccccgcggag
    41761 ggcgtcctcg aggagctcgc gcgtctcggt gtcgtcgctg tagccggccc ccgaaaggcg
    41821 taccgagtcc cccttcacca tcagctcgaa ggtaaaggcc gagacgaggt tcatcgcgcc
    41881 gatcacattc ctcacggcgc cgtcgttccc ggagatcgtg accgagccct tcgcctcggg
    41941 tagtttcgtc ctgtcgacca gcagccagcc ggaggcgttg ccgtccaccc ggtcccgaag
    42001 ggcgccgagg cccgcgcccc ggtcgaaccc ggtgccgccg gcggcgcggg cggcgagggc
    42061 cgccgcgaca ccctcctcgg acccggcaac gaggagccgg tcgctgacga aggcaaccgc
    42121 cccgccgccc cgcttcccgg tctgccggtc gtccttcagg aggaagtagg tcgcgccggc
    42181 ggccgtccgg gcctcggcgc cgcgggcgac ggcggcggcg gcgagacgcc ggggctcgaa
    42241 ccgcccctcg aaagcgacga gggcccttcc cgtcccgccg ttcgtcgcgg cgaagacgac
    42301 gaggtcgacg tcgtccaccg ggtggagccg cgcctcggag aggaaccgcg ccgcgtcgcc
    42361 gtcgaccgtc gcccggtcca tctcgtcgaa gaagcgtgcg gcgagcgggc tcgtccggag
    42421 gtccgagacg cggagcattc cggccgtcac ggcgtccgcc gggacgaggg cgagcgcgtc
    42481 gtcggccgga cggacggcgg cggccgggag cgcggtggcg aggagggacg ccagggcgag
    42541 ggaggcgatg cgcttcatgt tcagaagtcc tttctccgga agaagagggc cgacagggcg
    42601 agggagacga ggccgaagag gccggtcgtc ccgaagacgg cgaggtagtc gatctcgacc
    42661 ttgtgcctcg agagctgccc gccggaaacg aagtcgacgg tcgcctgggc gagctgcccc
    42721 gtcttcggga ggacccagta gaagccccgc acgagagcgg ccgcccattc cgtcgcgagc
    42781 gccgcctcga tctgcctgtg gcccgagagg atcgcggcga ggaagaagac ggcgaacgtc
    42841 gccatgatcg agaccgccgt cgacgacgtc acgacgccga cgaggaaggc gaacgccatc
    42901 agcgtcgcga tcgtgaagag gatgatcgcg ccgccgagga agaatcgtgg atggacgacg
    42961 cccgtcttcc accatatgag gagccccatc gcgccgacga ggtagacgac gttcagcccg
    43021 gcgaggagga gcccgccgac gtacctcgag aggaggagcg gcacgcggcc gacgggcctc
    43081 gagaggtaga ggtcgatcgt ccccttctcc tggagcctcg gcacgaggtg cgccgtcgcg
    43141 aagaccgcga ggaacgtcgc gacgacgtag aggaagccgg agaagcccga ctcgaacccg
    43201 aggacgagct tgtcaaggtc gaccgacctc ggccccatct cgagctgctt gccgaagagc
    43261 ttcgctccgg cgagcgcgcc gtcgacgacg tcgaggttca cggcggcggc gaagagggcg
    43321 atgaagagcg tcgagagggc cgcgtaggcg acgagcgtcc atcgcgccgc ggcctcgcgg
    43381 aagacctcct cgacgttcgc ggcgaggacc ttcaccgggg cctcccttcc tcgggcggcg
    43441 tgatcaccgt cccgccggcc tcgtccgtct cgcccgctcg gaccaggtcg acgaagacgt
    43501 cctcgagcgt cgagcggagc ggggtcagct cggtgacgag gaccccgcga gcccgcgccg
    43561 cgtcgacgag cgcgttcagg tgcgcggcgt cgcgcgccgt cagcttcacg tgcccgttca
    43621 cccgctcggc cgccgcgccc gactcccgga agagtccgag gagcgcctcg tccatcgggg
    43681 cgccgacgaa gcggtaggcg aacccctgcg cggtcaggtc ggcgatccgc ccggcggcga
    43741 cggccttccc cttccggagg acggccacgc ggtcgcacgt cgcctcgatc tccgagagga
    43801 gatgggagtt caggaggact gcggtgccgc gcgccgcctc ctcgcggagg aggtcccgga
    43861 tctcccgccg cccgaccggg tcgacgccgt ccgtcggctc gtcgagaagg aggaggtcgg
    43921 gacggtgcac gagagcggag gcgagtccga ggcgctgggt catccccttc gagaagcgct
    43981 tcaccttcac gtcgccccag tcggaaaggc ggacgcgggc gaggagctcg gggatccggg
    44041 ccttcaggtc ggaatcggag acgccggaca tccgcccgaa gaccgagagc gtctgccggg
    44101 cggtcaggta tcccgggaag cggtgcccct ccgggaggta cccgacgcgt cgccggctgc
    44161 cggggtccga gacgggaagg ccgaacagct cggcccgccc cgaagtgggg tgagtcaggc
    44221 ccaggaggat cttgacggtc gtcgtcttcc ccgccccgtt cggtccgagg agtccgaaga
    44281 cctcgcccgc ggcgacctga aggtcaagac cgtccaaggc tctcacctgc cgccgcgaga
    44341 acggggtccg gaaggtcttc gaaagccctt cggcggagag gacggcggtc atggctccct
    44401 ctacggtgtc gccgcccgac aggttcccac gcgggggcag gtgtgacgaa acgccccggt
    44461 cgcctaccgg agatcgaggg agaggcgctc ttctgtcttt ccttcggacg tcacctcgag
    44521 gacctcgagg tgaggcgccc tgccccgctc gaggcgagcc cggaggaatc cggctcggga
    44581 cgtcgcgtat cgggtcgagg cgagcttcga gacgacgcga ccggacggga agatcccgga
    44641 gcccgagatc aggacccagc gcgcctcggg caccggcgcc tcgaggacct ggagcgagtg
    44701 gtcgtgcccg gaggcctgga agagggccgg ccttcccgcc agcgcggagg cgaggtcgga
    44761 gcggagccgc cggtaacggc gcgaggcgag gtcctgcccg gtccccttcg cgccgcgata
    44821 cgccacccag gccgagccga ggcccgggat tggcacccag aacgacgggt gtgcgtcgcg
    44881 gagcgggaag acgtgggcct tccagtcgcg ggttcgctcg ccgtgcggcc cgcccgacgc
    44941 gagcgggtgg tgcgtcagga gcgcgagcgg gcccgcggag gcggccgttt tcagctgggc
    45001 cattgcctct tccgcgtcct ttgcggagca gggggagccc gctccttcag gcctcggccc
    45061 ctcgtgctgg agccaccact gtgtgtcgag gaccgcgagc gtgatccccg acggcgtcgg
    45121 gagaagaagc gggccagggc agccgtctcc cggggcgaag aggaggcgcg gggagcgggc
    45181 gacgaggaag cgttcctgcc tccggacggc cgcgaggccg tcttcccgca tcccgtccca
    45241 gtcgtggttc ccggggagga agaggacgcg ggcgccggcg ccgagcgcgg cgtccgcctg
    45301 ggccgcgagg cggcgctccc cttccgcgcg ggacgcggcc ccttcgggcg gcagaccgtt
    45361 cgggtagagg ttgtcgccga ggaagacgac gagcgtccgc tccggcgccc tcgcggccgc
    45421 cgcggcgagg gcttcgagga ccggctcggc cgccgccggg gcgccggcat cgccgacgag
    45481 aagaagcgtg tcgaccccgg ccgcgaaggg gctcgaaggg gcaggtcggg aaccgtcagc
    45541 gcggaggccc gccgtcgagg cgccggtgac gacgaggacg aggacgaagg ccgcgaacgt
    45601 cgcgaggccc cccgtaccgc aacttcgcgt ccggcggcgc ccgcgggcgc cgctcctggc
    45661 gccgggaaac gaccgcctca cctcgccgcg accttcggcg cggccccctt cgccgcgccc
    45721 cgcgccttct tccggagccc cgcccagagc tgccgaagct cgcccgcgag cttgtgcgac
    45781 tgggcgaagt ggacgagcgg cgtcgcctcc tggtgcgact cgcggacctt caccgaagcc
    45841 gagacgtacg gcgagaggac cggaagcccc tccgccttca gctcgtcgac gaggagctgc
    45901 gggagcttcg cgcggggctg gaaggcgttc acggcgatcc cggcgatctc gagcttcgag
    45961 ttgtggtcct gccgcacctc ctccacgacg tcgaggagtc cgtagagcgc gcggcgggag
    46021 aaatcgtcgc agtcgaacgg tatcaggacc cgctccgcgg cgatgagcgc cgagagcgtg
    46081 aagaagttga gcgccggcgg cgtgtcgaag aagacgtgag cataagaatc gagcggggcg
    46141 agggcgtcgc ggaggcgata gatcttgtgc cgcccttcga gcttcgcgtg gaacgtctcc
    46201 aggaccggct gcgtcgggag gacgtcgagc ccctcgaacg gcgtcgcgtg gatgaacgtc
    46261 cggatcccgt cgtcgacgag gcgaatctcg aggctccgct cgtagaagtc ggcgaggttc
    46321 gggcgagcgg ccttcgcccg gtcgccgaga agatagtggg acgagcagcc ctgcgggtcg
    46381 aggtcgacga cgagcgagcg ctccccctcg tcggcgagaa cggcggccag gttgcagacg
    46441 agcgtcgact tcccgacgcc ccccttctgg ttgaagacga cccacttcat ccgtcccctc
    46501 ccgtcgtggc gatccgggca tcatctcacc cgtcgcgcgc cccttccgtc tcgggaccgg
    46561 gcttctctag aatcgatctc gggaggaccg acatgcccgt tttcgagtac cggtgccagg
    46621 cctgcggaaa gaagaccgag gacctcgtcc tcgccggcga cgcggccgac tacgtccccg
    46681 agtgcgccca ctgcggctcg cacgacctct cccgcctcct ctccgccttc gccgcccacg
    46741 ggggcgacaa gggcgccggc tccttcgacg ccccctgcgg catgggcgct tgcgaaaccg
    46801 gcgacatgag ccagtgcggc atgggcggct gcgggatggg aggaggagat ttcgacctga
    46861 actgaacccc gtcttcagcg ccgccggcac ggattccgcc cgccggcgcc gtgctgcgct
    46921 gccgcgatta gaatcccggc cggtcctgta tccacccgac cgcccggagg aacgatgagc
    46981 gacagcttca agacgaagac caccctcacc gccggaggcg cgaccgtctc ctattactcc
    47041 atcgagacgc tcgcgaagga gcacccggaa gtccggacgc tcccctactc cctgaaggtc
    47101 ctcctcgaga acctcctccg ccacgaggac gggcgcgtcg tgaagcgtga ggacgtcctc
    47161 gccctcgcga agtgggaccc gaaggccgag cccgacaagg agatcgcctt ccaccccgcc
    47221 cgcgtcctga tgcaggactt caccggcgtc ccggccgtcg tcgacctcgc cgccatgcgc
    47281 gaggcgatcg tcgcgatggg gggcgacccg gcgcgcgtga acccgctctc ccccgccgac
    47341 ctcgtcatcg accactccgt ccaggtcgac aacttcgcct cctcgctcgc gttcgggaag
    47401 aacgtcgacc tcgagtacga gcggaatcgg gagcgttacg agttcctccg ctgggggcag
    47461 aacgccttcc ggaacttccg cgtcgtcccg cccggcaccg gcatctgcca ccaggtgaac
    47521 ctcgagtacc tcgcgaaggt cgtctggacg aacgaagagg gggtcgccta ccccgacacg
    47581 ctcgtcggga ccgactcgca cacgacgatg atcaacggcc tcggcgtcct cggctggggc
    47641 gtcggaggga tcgaggcgga ggccgccctc ctcggccagc ccgtctcgat gctgatcccc
    47701 gaggtcgtcg ggatgaagct ccacgggaag ctccccgagg gggcgacggc gacggacctc
    47761 gtcctcaccg tcacccagat gctccgcaag aagggcgtcg tcgggaagtt cgtcgagttc
    47821 tacgggccgg ggctcgcggc cctgccgctc accgaccgcg ccacgatcgc gaacatggcg
    47881 cccgagtacg gcgccacgat gggcttcttc cccgtcgacg gcgagacggt gcgctacctc
    47941 gagttcacgg gccgaaaggc gctcgccccg ctcgtcgagg cgtacagcaa ggcgcagggc
    48001 ctctggcgct ctgaggatgc tcccgagccg cgcttctccg acacgctcga gctgaacctc
    48061 gcggacgtcg tcccgtccct cgccggcccg aaacgcccgc aggaccgcgt cgccctcaag
    48121 gacgcgaaga aggtcttccg cgagcagctc cccgcgatgc tcggcaaggg aggctccgcc
    48181 gacgcgaccg tcccggtctc cgacggacgc ttgaagtacg agatgccgca cggcgccgtc
    48241 gtcatctcgg cgatcacctc ctgcacgaac acctcgaacc cgtcggtcct cgtcgccgcc
    48301 ggcctcctcg cgaagaaggc ggtcgagcgg ggcctcgaca cgaagccgtg ggtcaagccg
    48361 tcgctcgccc ccggctcgaa gaccgtcacc gactacctga ccgccgcggg cctcatgccc
    48421 tacctcgagg cgctcggctt ccacgtcgtc ggctacggct gcacgacctg catcggcaac
    48481 agcggcccgc tccccgagaa ggtctccgag gcggtccgga cgggcgacct cgtcgtggcc
    48541 tcggtcctct cggggaaccg gaacttcgag gggcgcgtaa atcctcaagt caagatgaac
    48601 ttcctcgcct cgccgccgct cgtcgtcgcc tacgcgctcg cgggcgagat ggacacggac
    48661 ctgacgaccg agccgctcgg catcgaccgg aacggccagc ccgtcttcct ctcggagatc
    48721 tggccgacgc cgcaggaggt tgccgagacg atcgcgcggt cgctcacggc cgagcagtac
    48781 gagaaggcct acggctcggt cttcgcgggc gacgccggct ggcaggccct ctccgtcccg
    48841 aaggggagca ccttcgcctg ggacgcgaaa tcgacctacg ttcgcaagcc gaccttcttc
    48901 gaggacctcg ggaaggagcc gaagcccctc gccgacgtga agggggcccg ctgcctcgcc
    48961 ctcctcggcg actccatcac gaccgaccac atctcccccg ccggcaacat cgcgaagacc
    49021 tcacctgcag ccaagtacct cacggagaac ggcgttcagc ccgccgactt caaccagtac
    49081 ggcgcccgcc gcggcaacca cgaagtcatg atgcgcggca ccttcgcgaa catccgcctc
    49141 aagaacctga tgctccccgg caccgagggc ggcgtcaccc tccacgtccc gacgggcgag
    49201 acgatgtcga tctacgacgc ggcgatgaag taccaggcct cagggacgcc gctcgtcgtc
    49261 ctcgccggcg ccgagtacgg caccggctcc tcgcgcgact gggccgcgaa ggggacgatg
    49321 ctcctcggcg tcaaggccgt catcgcgaag tcgttcgagc ggatccaccg ctcgaacctc
    49381 gccggcatgg gcgtcctccc gctccagttc gccgcgggcg aggacgctac gacgctcggc
    49441 ctcgccggga aggagacctt cgaggtcgcc ggcgtcgccg aggggctcac accgcacaag
    49501 cagctcaccg tcaccgccac ggccgaggac ggcacgaaga agaccttcac cgccaccctc
    49561 cgcctcgaca cgccgaacga ggtcgactac taccgccacg gcggcatcct gcagttcgtc
    49621 ctgaggcaga tgacggcgta ggcccccggg ccggaccgag ccggtcgcga gggccgcggg
    49681 atcgcgcccg cggccctccg cgtctccggc gcccgctctt gcgctccgga cgcgagcggg
    49741 tacagttcca cccgatgggg ctgttctccg acggaccgaa gggcacctcg ccggagctga
    49801 ggcaggcgat cgaggacgcg cgccggcgtc tcgaggaaag cccgacggac gcggcggcgg
    49861 tgttgaagct cgcggacgcg ctcgtcgcga gcggccggaa gaccgaggcc gtgagggtct
    49921 tgaaccgctt cggcccgatc gttcagtcga aggggcggct cgaggcggcg atcgccatct
    49981 tcaagaaggc gacgcagctc gatcccgaca gcgagctgac gtcgtcgacc tatctctcgc
    50041 acctccagct ccagcagatc ctcgacgccg aaaaggccgt ccgtgccgcg acgtcccgtc
    50101 ccgcgtctcc gcccccgtcg ggctcgttca cgcgtccggg ccccgacccg ctccctccgt
    50161 ccggcagcgc ctttgagctc gccgcggccc ctcctgtcgc ggagccgccc gccggcccgc
    50221 cgccgagcgc ccccctcccg agaaccgcag ccgctgtccc ggcctcggac gactcggacg
    50281 cggcgtccgc gccggcgcct tccggccctt ccgcctggag ccagaagaag gaggccgtcc
    50341 acgacgcgca ggccggcatc cccctcctgc gcgacatccc accgttcctt ctcgacctcg
    50401 tcctccagcg gatcaacctg atcacgctcg ccccgggcga cgtcctgatc cgcgaaggcg
    50461 ccgaagggag ctccgtctac ttcgtcgtcc aggggacgct cgccgtgacg gcgcggcacg
    50521 acctcgggac cgaggtgctc ctccggacgg cccgggacgg cgaggtgatc ggcgaggccg
    50581 ccttcctcac cggcctcccg agctacgcca cggtgacggc ccgcgagccg tcgaacctcc
    50641 tcgagctcga ccggaatgcg ctcgtcccca tcgcccgcaa gaaccggacg ctcgccgacg
    50701 ccctgaaccg cctctacgag gagcgcgtcc tccattcggc cctcgtccgc tcccgggtct
    50761 tcggcgtcct ctccgagccg gagctccggc tcctggcccg gaagctcgag acgacggccg
    50821 tgccggcggg aacgctcgtc gcccaggaag gcgtccccgc gaagggagcc tggatcgtca
    50881 agcgcggcgc gttccgggtc accttccgcg ccgacggcca cgaggtcgca gtcgcgctcc
    50941 tccggccgca cgaggtcttc ggcgacctcg cggaggggcg tgaccttcct caggccgagt
    51001 cggtcacggc gatcgccgag tcggagctcc tcttcctccc ggcggaggac ctctccacgt
    51061 tccgcacgcg gagcgtacgg ctttcggagg cccttgacgc tctccggctc gagcgcgcga
    51121 agatgtgcgt ggcggcgctg cgcgccagcc gccggtagcc ggcccgcgcg ccgggccgct
    51181 ccatccgtcc tagactgtct cgcgcacgac gaggaggtgc cctatggccg gcatcaaggg
    51241 tatcgaaggg atcggcgaca cgtacggcga gaagatgaag gcgctcggca tccgctccaa
    51301 ggccgccctg ctgaaggccg gcgcgacgaa gaaggggcgc gccgatctcg cggcaaaggc
    51361 ggggatctcc gagaagctga tcctcgagtg ggtgaatcgc gccgacctct accgcgtgaa
    51421 ggggatcggg acccagtacg ccgacctcct cgagaacgcg ggcgtcgaca ccgtcgtcga
    51481 gctcgcgggc cgcaaggccg agaacctcgt cgagaagatg aagcaggtga acgccggaag
    51541 gaggctcgtc cgcctcctcc ccgcgctctc gaaggtccag ggatggatcg ccgaggcgaa
    51601 gaagctcccc cgggccgtca cgtactgacc gaccgcgccg cggacgagac cgtccggcga
    51661 gggaacggcg gtcgctggca ccggggtgtc gttcgacgcc tcctggcgct cgtgctggtc
    51721 gcctcgccgg cgttccccgc tcgcgccgag gtccgcgccg tccgcctctc ccgagaggga
    51781 gccggcttcg ccgaggggcg gctcgaagcg ggcgtcacgg tccgtgccga cggccttcac
    51841 gccggacgag gcggcctcta cacctcggcg gcgatcgcgg ctccgatcgc cttttccgcg
    51901 atcggcccgt actggaaggg ggcgccggac gtggcggtcg aggtcgccgt cagcccggac
    51961 ggtctcgcgt ggggacgctg gatcccggtt cccgccgagg agtccatcgc accgctgcgc
    52021 gaggacggct cgcccaatcc cttcggcggg gacctcctcg gcccgctcgt gttcgtctcc
    52081 cccggcagcc ggtggttccg ctaccgcgtc cgtcttccgg cccgtcggga cggcgaaccg
    52141 tctctctctc ggatcgcctt ccagctgatc gatccgggac ggaccgcgga cgaggacgcg
    52201 ccacaggtcg aagcccgcgt ggagagctcg ctcgccacgt ccggcccccg ccgtgccgtc
    52261 gccccggtcc cggaagagcc ctcgccggtc gcgagagcgg accgcccgcc gatcctccgc
    52321 cgcgcggcct ggggggcacg accgccgaag tacgcatact cgtacacccg cgccggccac
    52381 gtcggagtcc accacacggc ctcggtcgac gacttcaccc cgacgacccg cgccgagtgc
    52441 gccgcccggc tgcgcgcgat ccaggcgttc cacatggaca cgaacggctg gaacgacatc
    52501 ggatacgcgt gggtcgtctg ccggcacggc gaggtcttcc aggggcgcga ggacgacgac
    52561 gacgcgacgg acgtgcaggg ggcgcacgac ggattcaacc gggggagcac gggcctctcg
    52621 ctcctcggct acttccatcc gccgtacgac cagcacccga cccaggagca gctctcctcc
    52681 ctcgtcgacc tgatcgcctg gatctccggc ctccgcggga tcgacccggc cggccggagc
    52741 ctctacgagg ccttcggcgc cgacgtcgac aacgtctacg gccaccgcga ggtcaaggac
    52801 acgagctgcc cgggcgatca cctcttcgcc ctgaaggagc tcctgcgcgc cgccgcgctc
    52861 gagaagctcc tcatcggggc gctctgagcg ggccctcccg tcccagcgct cgaacggacg
    52921 acgggcggcg cggaacgggg cgttccgacg tcacgtcgct ccgggctcgt cgttagaatg
    52981 ccctcgtgat cgacgtcgcc cggggctgcg tgagccgtcg cccggcgttc ggcgagtccg
    53041 ccgatcgcgg aaccgtcgga cggacccgcc gcggatgagc cggacgggcc ggattcgtct
    53101 ggaggcgttc gaggtcctcg ctctcgtcgc cttcgtctcc acggtggtga ccctgctcgc
    53161 gggcggcctg cgcctgacgc cccggacctt ctggttcatc gtccgcgaga cgggtccgtt
    53221 cctgctgctg ttcctcggca tcggcgtcgt cgccaacgtc ctctaccggc tgcgtccggc
    53281 cggtgccctc cggccgtacc tcgagagcct tcgctccccc tcctggcttc tcctgacggc
    53341 ccggatctgg gtcgccggcg tgctgctcct ctacgcctac acctggctga aggtgtcgat
    53401 cccgctcctg aacgagcgtc tctgggacgc cgagctggcg cggctcgacc agacgttcca
    53461 cgccggcctc tcgccgagcc ggttcctcgt cgccctcttc gaggggaccc cggcgctgcg
    53521 cctcctcgac ggcgtctacg gcatctggct gccgatgacg atgagcggcg ccgggttctt
    53581 cgcgatgacc ctcagtcccc gtgcgcgcgc gcagttcttc ttcagctacg tgatgatctg
    53641 gacgctcggc gtctggacgt acgtcgccct tcccgcgctc ggtcccgcgc tcgcgtaccc
    53701 ctgggagtgg acgaccgtgc acgccgacct gccgcaggcc ctcgaaacgc aggcgctcct
    53761 catgggcaac gaccagaagg tcgtcgagag ccgcgtgacg ggcgtcctcc gcgcggggtt
    53821 caatccctcc ctcggcgtcg ccgcgatgcc gagcctccac acgtcgggat cgacgcgctg
    53881 ctgctcctct ggtgcttccg gcacgcccgc ccgctcgcgg cggtgttcct cgcggcgctg
    53941 cttctcactc tccgcgggtc gatcgtgacc ggctggcact acgcggtcga cgcctgcgtc
    54001 ggcggcctgc tcgccgccgt ctcgttcgtt gcctcgagaa gggtgtcgct ccacctctcc
    54061 gacccggagg agagcccgcc gtcgaaaacg ccgctccagg gagtcgaccc atgaccctcg
    54121 acatctccct cgagcccgcg ctcgacctcg ccgcccgcgc cgtcgaagag gccgacgcga
    54181 tcctcgtcac ggcgggagcc ggtatggggg tcgactccgg cctccccgac ttccggggcc
    54241 ccgagggctt ctggcgcgcc tatcccgcct tccgcgacct cggcctgcgc ttcgagcagc
    54301 tcgcgagccc gcactggttc ttccgggacc ccgcgctcgc ctggggcttc tacggccacc
    54361 gcctcggcct ctaccgcgag accgtccccc acgagggctt cgggatcctc ctgcgctggg
    54421 cctcggagaa gaggcacggc gcgttcgtct tcacctcgaa cgtcgacggt cagttccaga
    54481 aggcgggttt ccccgacgga cgcgtcgcgg agtgccatgg ctccatccac cacctccagt
    54541 gccttcgcgg ctgcggcgag ccgatccggt ccaccgacgg cgtcgtcgtc gaggtcgatg
    54601 cggcctcctg ccgcgcccgc gagccgttcc cgtcgtgccc cggctgccgc ggcctcgcgc
    54661 gcccgaacat cctcctcttc ggcgacctcg gctgggacga cgcgcggacc tcggcccaga
    54721 tggagacgct cggcgactgg ctcgacggcg tcgcgggccc gctcgtcgtc ctcgagctcg
    54781 gcgcgggggc gagcgtcccc tccgtccgga cgttctccga gcgggcggtg cagggcaccc
    54841 ggcggacgct cgtgaggatc aacccccggg agccggagct cggccgcggc ttcggggcgc
    54901 cggcctcgga ctccctcgag catctccttt cggcctcgag gcaggccgcg cgcccgtcgg
    54961 ggatctcgct cccgctcggc gccctcgagg cgatccgcgc gatcgacgcc cggcgcgggc
    55021 cctcccgccg ctgagctccc gctttcggcc cccgggccgg tccggtcgcg cgttgtcgcg
    55081 cgtttgcacg tgtgctgata ccatgctctg atccgaaacg attacccggg aaatggtgaa
    55141 atctcatcag gtccgggccg gtaaactcct cgtcggagga ggcaccatgc ccgccctgaa
    55201 ggaaagaccg aaaccgttcg tcctccccga gttttgcaag ggttgcggac gctgcatcag
    55261 cgcctgcccg aaacactgca tctcgatggg aaccgagatc aacccgaaga ccgggctcac
    55321 gccgatcgtc ctcgacctga cgaactgcaa cgggtgcgga ctctgcatcg acgcctgccc
    55381 cgagccctac ggccttcagc agaccccggc cgacgccgac tacgagctcc aggacccggc
    55441 gaagctcttc ggcgaccgga tgtccgacgc ccccgagccg gtcgacatcc ccgaccaggt
    55501 cgtcccgctc ccgacgatgg agccgctcgt cctgaagggg aactacgccg ccgccgtcgg
    55561 cgcgctcctc gccggctgcc gacacttctt cggctacccg atcactccgt cgaccgaggg
    55621 cgccgagctg atggcgaagc tcatgcccca cctcgacggg acgttcctgc aggccgtctc
    55681 cgaggtcgcg accgtcaaca tgatgtacgg ctgcggcggc gcggggatgc gctccatgac
    55741 gttcacctct tcgcctggct tctcgctgat gctcgagggg atcagctaca tgatcggcgc
    55801 cgaggtcccg ggcgtcttca tcaacgtcat gcgcggcggg cccggcctcg gcaacatcgc
    55861 ccccgagcag tcggacatca agctctgttg ccgcggcctc ggccacggca acacgcacgc
    55921 catcgtcctg acgccgtcga ccccgcagga gatgctcgac ctgacgatgc tcgcgttcga
    55981 gctgacgttc aagtaccgca acccggtcgt catcgcctcc gacggctacc tcggccagat
    56041 gaccggcaag gtctccctcc ccgagtcgat ggtgaagccg ggcatcccga agtgggccgt
    56101 ctacggcgac ctcgagcacc gccggaacct gatctcctcg atctacctct ccgaggtcga
    56161 tctcgagaag tggaacgtcg tgctgaacgc caagtacgcc tcgatgcagg tcgagcagcg
    56221 cgccgacctc tacatgtgcg acgacgccga ggtcctcctc gtcgcctgca acacgccggc
    56281 ccggaccgcg aagggggccg tccaggtcct ccgctcccgc ggcatcaagg cggggctctt
    56341 ccgccccgtc acggtctggc ccttcccgat cgagaagctg aagacggtcc tcccgaacgc
    56401 gaagcgcatc gtcgtcgtcg aggcctcgaa cggccagctc gaggacgagg tcaagattgc
    56461 cctggcgaac gcccggatcc gcgagtaccc ggagatcgag cacgtccgcc acttcggcgg
    56521 cgtcctgcct cagatggagg aggtcatctt gaaagtcgcc gcggggcagg aggtgaccgt
    56581 atgacgacgt ccgccttcta cggcaagttc gagcgccact ctcacggcga ggggctgaag
    56641 ggccattcca cccactactg cccgggctgt ggccacggcc tcgcccacaa gtacctcggc
    56701 gaggcgatcg acgagctcgg catccaggac cggaccgtcg ccgtctcccc ggtcggctgc
    56761 tccgtcttcc tctactacta cttcgacgtc ggcaacacgc aggccgccca cggccgcgcc
    56821 cccgccgtcg cgatcggcca gaagctcgcg aaccccgagt cgatcgtcat ctcctaccag
    56881 ggggacggcg acctcgcctc gatcggcctc gccgaaatcg tctccacggc ccagatgggc
    56941 atcccgatca ccgtcatctt cgtcaacaac gcgatctacg gcatgacggg cggccagatg
    57001 gccccgacga ccctgatggg tcagaagacg gccacgagcc ccgacggccg ggtcgacttc
    57061 atgggccagc cgatcaagat ggccgagctg atcgccggcc tcgacggccc cgtctacgtc
    57121 gagcgcgtcg ccctcttcaa ccccaagcag cgcaaccgcg cgaagaaggc gatccacaag
    57181 gcgctccaga accaggtcga gaagcgcggc ttctcgttcg tcgaggtcct cgccgagtgc
    57241 ccgacccacc tgaaggccac gcccgagcag gccgagaagt gggtcgagga ggccatgctc
    57301 ccgatgttcc cgctcggcgt gaagaaggac gagacccgcg aaccgtggtg ggaccggccc
    57361 gccccgacgt tcgagaccga gcgcgtcctg aaggagatcg gcgccggcga ggagaaggct
    57421 ccccgctact gcacgtcgtt cccgtcccac gtcgcgaaga acgacatctc tctcaagctc
    57481 gccggctccg gcggcgacgg cgcgcagacg gcggcgatgc tcatcgtcca cgccggcatc
    57541 aacgagggct tcgacgcgac gcacatcc
//
LOCUS       DAOONL010000014        56623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_378_length_56623_cov_16.143845, whole genome shotgun sequence.
ACCESSION   DAOONL010000014 DAOONL010000000
VERSION     DAOONL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 56623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_56623_cov_16.143845"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLP50_03935"
     CDS             complement(<1..880)
                     /locus_tag="PLP50_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50731.1"
                     /translation="MPNEVTVERVVPGGSGLARIDGAVVLVDGGLPGDRLLVEARSDG
                     PRLLRAEVVSVLAPGPHRRPPDAVCPLARDGACGGCDWPAARIESHGALKEELVRDAL
                     RRIGRVPDGELPDVRFVASPRSYRLRSRLRWDGGGALGFLGRGSSRVSDLEACEVVST
                     ALLERLPAIRAALGTANVPACELQTLEGTDGSPLLGELRFDRALPDPERIAAALHGPL
                     DGLRVLFSGRLAATRGPSSLRLVVAGAPFRVSVSSFFQANRFLLDAFLEEARAAAREA
                     ARAGVSRGALDLYAGAG"
     gene            complement(884..1609)
                     /locus_tag="PLP50_03940"
     CDS             complement(884..1609)
                     /locus_tag="PLP50_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50732.1"
                     /translation="MTGTPTPAPPGAPDAAEDEASYGTRVEEAFIAERGTPFLLSPKD
                     WRLICGWRESGVPSGTVIRAVREAFERKRARGDLSKISSLSYCANAVEVLWEMERRGL
                     VGAHSARAETSPDASDAAPRLAALAEALEVAEANVPGEADPAVVRAALAAARTALGEL
                     DPSSGLESLEESLGGLEKALVKRVEKALGEAGRTALAEEVAEEAGDLSGLSKDARGRI
                     QRALRARAVRRRLGLPALTLLGG"
     gene            complement(1569..2366)
                     /locus_tag="PLP50_03945"
     CDS             complement(1569..2366)
                     /locus_tag="PLP50_03945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPA50733.1"
                     /translation="MGLLLALDVGNTNTVLGFFEGDALVATARLTTVRDRTSDEHALF
                     LRAVIEGKGRKAGEVEDVIVSSVVPPQDFPIRVACRDAFGIDPLFVAPGVKTGMKILY
                     DQPSEVGADRIVNAVAAVAKYGAPCIVVDFGTATTFDVVSAAGDYLGGVICPGPGISA
                     DALFSRAARLSRVDLHRPEKVVGKNTAQSIQSGLYWGVLAQAEGLIARIRAELGEPGM
                     KVVATGGLAPLYASASPDLAAVDADLTLRGLKILHDRNADARPPRRS"
     gene            complement(2374..3168)
                     /locus_tag="PLP50_03950"
     CDS             complement(2374..3168)
                     /locus_tag="PLP50_03950"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPA50734.1"
                     /translation="MTSPLGPLAPLGGARFGRVANVVLLEETASTNDVARALVEKMVA
                     EDTDLLPTCVAARRQTAGRGRAGRSWSTTGEGAVAVSLVAPWPEGPGRIHVPVATGIV
                     LARSLSAAFGLDLRLKWPNDLFCRGKKLAGILVEGRALPEGAGYVVVGVGLNVSTTRS
                     ELDALGLAAATSLALEGVEPALLSGDLPLVAVISALDATLEGEVEDLPAAFAAVTLHR
                     EGDPLEVRDGERVVSGRYRGLTKDGLLRLEADGGVAELISGDVTRF"
     gene            complement(3165..5843)
                     /locus_tag="PLP50_03955"
     CDS             complement(3165..5843)
                     /locus_tag="PLP50_03955"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPA50735.1"
                     /translation="MSSAPRPELDKRFDPQAFEMKWYRLQEESGQFRPEVAAEDARPY
                     TLVIPPPNVTGRLHVGHALGRTLEDVLARWRRMQGRAVLWVPGVDHAGIATQMVVERD
                     LTERTGKTRHDLGREPFLEICREWADARRGDILNQIRRLGCSCDFSREYYTLDAARSK
                     AVRRVFSELYHQGLAYRDSRMVNWCPRCATVLSDLEVNHVESSGQLWSVAYPLEDGSG
                     EVVVATTRPETMLGDTAVAVHPEDERYTAIVGKKVRLPLTERLIPVIADEMVDRKFGT
                     GVVKITPAHDPNDFEAGRRHGLPQVVVIGFDGKLTAEAGAAYAGLERTAARKKVLADL
                     REQGLRRDDKPHTSAVGRCQRCDTVLEPLVSLQWFVKIKPLADKAVAATEAREVTFTP
                     ELWKKTYDEWMKNIRDWCVSRQLWWGHEIPAWYCPEGHVTVPKPGDEADPAACAVCGS
                     AKLERDPDVFDTWFSSWLMPLSVLGWPERTKDLARFYPTDVMVTGFDILFFWVARMIM
                     AGVWFDGKVPFREVFLHGLVRDERGQKMSKTKGNVIDPLVMCDEYGADAVRFALAVLS
                     GTGRDLPFGTTRVAASRAFATKVWNAARFAMGMLGETPVASEPDFASLGRVDRWILDR
                     LATTAKKVNDSLEVFRFDEAANALYAFFWTEFCDGYVEMVKQVLRDETLPEEEKAKTR
                     AVLRHVLLDSLALLHPFMPFVSCEIREALNGDGAKLPVLPYPAGRDDWRDPFAVEVVE
                     TLRETATRIRNLRAERGLAQTEALDAGLEVPEGEVAEALRAHAPLLSHMARLSSLVVA
                     PKVDLPNAFRDHVGVAGLVVALPKKELSAEEEAKARKEIASLDKEIEGVSRKLADESF
                     LSRAPQAVVEKARKQLEELAARRAKLVTNLGEAGAR"
     gene            complement(5891..7885)
                     /locus_tag="PLP50_03960"
     CDS             complement(5891..7885)
                     /locus_tag="PLP50_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA50736.1"
                     /translation="MSIKILLADDSLTIQKVVELTFSDAETRLLSVGSGDKAVQALDE
                     FQPDIVLADVVMPGLTGYDVCEAVKQRPGGAFVPVVLLTGTFEPFDRARADRVGSDAI
                     VTKPFDSHALQGLVRELVLKGRAAREAAESAPPPAPERPAPPAFTLADLDAPPEAAVP
                     VDTGSTLVLTPLDLDQSAPPPFVEAATAAPAGESIYATMAMKLPTPEELAAAAPAPFE
                     LAEEAPIADVPAPFEPAEPLPEPFAAVAATLPPEAPFDVDGASVAAPAVEAAPPVVET
                     SPWNEEPTQESAPPAEPAFLVEPPPPPPYDPLAAYIPPPPPPPFEAAVVFVPPPPPPP
                     PAFYEPEPPVPPPPPRFYEPEPATTDEAVPDGPDTAPVLPLVSPAVPEAEPFEGAPAE
                     DAAAPVEPPSDAPFETELPPELEAERPALVETGAEEGTGPAEPFEERSTGEAISREET
                     GDEAPAEAVEPFPEMEAAAQVADGAPWEEGPALQVPPPPALDARGDDLSDEPEPVTRD
                     LEADMEAFEASGRVRRRPEIWERHAALIGEEVPEDIDVAGVVPEAPAATRGEPGSADL
                     EEIAAAARLEDLSALIPAGAASSPGEPAVVPEPVAAVTTAPVVAAPGGGLTDADVDRI
                     ARRVVELLSDKVVRDIAWEVVPEYAERLVRERIGEVERAG"
     gene            complement(7959..8891)
                     /locus_tag="PLP50_03965"
     CDS             complement(7959..8891)
                     /locus_tag="PLP50_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPA50737.1"
                     /translation="MTGAGRVTVVEVGPRDGLQNERGFVPTDVKVAFVEALADAGLPV
                     VETTAFVSPRAIPQLADSAEVFARVRKRPGTRYPVLVPNERGMGRALAAGAREIALFT
                     AATDTFNLRNVNATVEESFARFAPVLDRARGEGLRVRGYVSTAFGCPYEGRVDAAKAA
                     VVAARLFAAGCQEVSLGDTIGVAVPSQVTDVLGKVREAGVPIERIALHMHDTRGTALA
                     NVVEGLREGVRVFDSSAGGLGGCPYAPGAAGNLATEDLVYLLHAMGWETGIDLEKVSA
                     ASEALAAAVGRMPVSRVFAAMRAASERASKTASR"
     gene            complement(8888..9274)
                     /locus_tag="PLP50_03970"
     CDS             complement(8888..9274)
                     /locus_tag="PLP50_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50738.1"
                     /translation="MKIRNLSTGTLHELAPGPGEPPRGARVVRDGSVVWVWHRGETWR
                     LARVVEKPGASEEEEHDLRAPMPGVVARVLAEEGQEVVKGTPLLVLTAMKMEHEIRSP
                     RPGRLAKLFRAAGERVDLGDPLAEIE"
     gene            complement(9271..10746)
                     /locus_tag="PLP50_03975"
     CDS             complement(9271..10746)
                     /locus_tag="PLP50_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPA50739.1"
                     /translation="MNVRPLRKVLVANRGEIAVRVLTACRTLGYETVAVVSEADVGSR
                     HARLADEVVVLGPAPARESYLVAAKVLDAARATGAEAIHPGFGFLSENAAFARAVIGA
                     GLVWIGPPPEAIEAMGLKVPSRERMRAAGVPVVPGTHDLSAEGIAKVGFPLVVKASAG
                     GGGKGMRVVRAEGELASAVASCRAEAEAAFGDPTLYAERYLEHPRHVEIQVFGDAHGR
                     VVALGERECSLQRRHQKVVEESPSPAVSPELRARMSEAAVAAAKAVGYVNAGTVEFLL
                     DADGSFFFLEMNTRLQVEHPVTEEAWGLDLVALQLAVASGEPLPADVPAAPVAHAIEA
                     RVYAEDPEAGFLPQVGTVLVYEEPVGPGVRVDSGIEAGSEVSVHYDPMLAKVVARGRT
                     REEARLRLVDALSRTVILGVATNVSWLRRLLGTPEFVRGELDTSLLGRLNVPPPHDPP
                     PDVLAAAARFLGGGDGQVARSATARAAFPDPFNEGSFRVLG"
     gene            complement(10743..11549)
                     /locus_tag="PLP50_03980"
     CDS             complement(10743..11549)
                     /locus_tag="PLP50_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPA50740.1"
                     /translation="MSASLRLERTRAGVVRVVLARPELRNAFDDALIAEVTAAFEELA
                     SDASTRVVVLAGEGKSFCAGADLTWMKRMVAWGEEENRRDAEALGRMFLAIDSCPKPV
                     VGRIHGAALGGGTGLVAVCDVAVCAEGTLLGTTEVRLGIVPAVISPFVVRKVGESHAR
                     RWFLTGERFGAEEALRAGLVHAVVADAELDAEVERVIDALLLGGPEAIAVSKGLAMRM
                     GRVPLDEAVAEAAGVIAARRVSAEGQEGMNAFLSKRAPAWTRDQERRSGS"
     gene            complement(11546..12385)
                     /locus_tag="PLP50_03985"
     CDS             complement(11546..12385)
                     /locus_tag="PLP50_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPA50741.1"
                     /translation="MNRRRFLRILLASLLASLPASAAAPPKGTIRSIEVTALTRQKAF
                     LFVRTTLPRAARGATQKFRGETTAWGAPVPLVQPVKVAVQPSANGFDVVFLAEIDLAA
                     VPKAIVERGSPVTLPVTLKGTLAGEAGTAATVDAAGALRPGTADLVAHRELADGFVRF
                     AGSRISGLGLAETKGEARLAVFNPFRFDVSLRGLRYELASGGRVVARGSRQGVIVRPS
                     RENEIALPLTVSNAAALAAAGKAVTSGGRLSGRLTGEIVLKLGTGDVKLPIAASGTIE
                     VLR"
     gene            complement(12394..14007)
                     /locus_tag="PLP50_03990"
     CDS             complement(12394..14007)
                     /locus_tag="PLP50_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPA50742.1"
                     /translation="MSDVLVSRVDSTSEDYLQNRVHNEGLARELRERVATAREGGGTR
                     GAARQAELGKLSVRGRIERLLDRETAFLELSPLAADGMYDGQAPGAGLVTGIGRVSGR
                     DVMVVANDPSVKGGTYFPITVKKHLRAQEVALENHLPCVYLVDSGGAFLPLQAEVFPD
                     RDHFGRIFYNQARMSAAGIPQISAVLGSCTAGGAYVPAMSDEVVIVKGQGTIFLAGPP
                     LVAAATGESVTAEELGGGDVHTRISGVADFLASSDAEALEMVREIVARLNTIKTVSGD
                     VVQPEEPLYAPDELYGLLPRDLKRPLEIREVLARLLDGSRLHEFKPRYGSTLVCGFGR
                     LHGMPVGVLANNGVLFSESALKGTHFIEMACQRRVPLLFVQNITGFMVGKAYEHGGIA
                     KDGAKMVHAVANAAVPKMTLLVGGSFGAGNYGMCGRAYQPRFLFSWPGSRISVMGGEQ
                     AAGVLSTVKNEQRQRQGQPPMSPEEEAVFKRPTLEKYESEGSPYYATARLWDDGILDP
                     AETRKVLSLALSVCHNAPIAKTSFGVFRM"
     gene            complement(14078..14497)
                     /locus_tag="PLP50_03995"
     CDS             complement(14078..14497)
                     /locus_tag="PLP50_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPA50743.1"
                     /translation="MTGRPLRVLIAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PEQVAAAAVQEDVDVVGLSILSGAHNVLVPEVISALGRRGAEGVSVVVGGIIPEKDIP
                     GLTAAGVKAVFPPGTTTTAVIDSVRDLARPHEAAAVR"
     gene            14549..15814
                     /locus_tag="PLP50_04000"
     CDS             14549..15814
                     /locus_tag="PLP50_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50744.1"
                     /translation="MRRRIGDVLVGGKAISQELRDRALALQLKQKGRLCTNLLELGGV
                     REDLLLRALAVQYGVATCTAADLAEIRPDILRLVPAKLAANLFVVPFKRLGRNLSLAM
                     RDPKDLPAIDEVSFLTGLAITPFVALDLRIQLALQKHYGIAIDPRYADLAARLEAGGG
                     VLPVPPPRTPRPLVRSSSAGTRAVAPESPPVPVPAPPAAQPAPLPAPAPDPAPPTAPK
                     PAAPAEIEMEAHEEERLRISGPIVLPPEPTDAVGGEEVAPLVAEAAPASLVDCLSRAE
                     SRDEIAAAVLREAGRLLRRAALFIAQAERVIGWAAVPEPPDGLRSFSIGFAEPSLFAS
                     LRNTDGFYVGPCPDLPGNRKILEALGSDYPAHVAAVPVTLKGKSVLFLLGEVRPGETP
                     PKAMELKRLGALTAIALEILLLRNKLRSL"
     gene            15842..16174
                     /locus_tag="PLP50_04005"
     CDS             15842..16174
                     /locus_tag="PLP50_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPA50745.1"
                     /translation="MIKNDLVNRVTEETGVPRVQASRAVETIIEAMRRSLAGGGRIEL
                     RGFGVFLVKRRKRGIGRNPKRPDEQVEIPPGFTIRFKPGKELRDLMIVEPAAPAAEPA
                     VPSTPEPA"
     gene            complement(17167..17622)
                     /gene="dtd"
                     /locus_tag="PLP50_04010"
     CDS             complement(17167..17622)
                     /gene="dtd"
                     /locus_tag="PLP50_04010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPA50746.1"
                     /translation="MILLLQRVSRAAVRVGGEAVGEIGRGLLVFGCVEPGDGPDAMDE
                     AARKTVELRVFEDEKGKMNLDVTQVGGAVLAVSQFTLGADLSRGRRPGFEGAARPEEA
                     RPLFDRYLAALRASGVPVATGSFGAAMEVELVNDGPATFHWRAKRRDVI"
     gene            17705..18052
                     /gene="ssb"
                     /locus_tag="PLP50_04015"
                     /pseudo
     CDS             17705..18052
                     /gene="ssb"
                     /locus_tag="PLP50_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(18134..19198)
                     /locus_tag="PLP50_04020"
     CDS             complement(18134..19198)
                     /locus_tag="PLP50_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPA50747.1"
                     /translation="MSGARLVVGLAGTALSVAEKRFLAERRPAGVLLFPENLASAEQA
                     AALVADVRAACDPSPLLFVDQEGGPVDRLGPLLGFDVPSPAALSAIGVDRVHEGAYLM
                     GRAARLLGFDVDLAPVLDLAQPGTGAVVLAGRTFGFHAEDVVVAGSVFLHGLARAGVA
                     SCVKHFPGLGRGPVDSHGALPVVDAHDVDLMVTDVAPFTKLVRASDAVLVGHAAYPGF
                     TGDETPASLSPRIHAILRERIGWDGVVLSDDLGMGALAEIPFPERAVRAARSGCDLLI
                     ASQGLDAAAAAAEALAAERIDREPAALERIAALRRRCDGHPRTRFGPEAWNALRAEGE
                     SWLEDLAKPRRRRDDADFGF"
     gene            complement(19195..21813)
                     /gene="mutS"
                     /locus_tag="PLP50_04025"
     CDS             complement(19195..21813)
                     /gene="mutS"
                     /locus_tag="PLP50_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPA50748.1"
                     /translation="MRTTPMLEQYWALKKEVPDALLLYRLGDFYEMFFEDAEAAAPLL
                     GLVLTARHRDTDVEAPMCGVPHHALESYAARLIAAGMKVAIAEQVEAPQKGKALVARR
                     IVRVITPGTLLDPERLDARQANDLAAIALGGDEAGLAFLDLSTGEFTAVRLDGTPPAE
                     LLARRPARELVAFPSERDDVKRLLEALGPEAPVLSVLTSESPRGRSAAELLEGHFRTS
                     TLSAFGLPASGPLVEAAAAALAYARSTQRSDCTHVTGLRTESATGGLVVDAVTQGHLE
                     LFRSLRDGSRTGTLLSVVDRTGTAFGARALRRILEQPLGSVEEIDERLDAVEELVTGP
                     ARLEAVATALSRLPDLPRLLSRVAVGSATPRDLASLGTGLEQAAAVGPILEGAESALL
                     RPGGRAFPSGVPDALSARLAATLVETPPASAKEAGLVRDGADAAVDELRVLRRDAATL
                     LGALEAEERSRRGIPTLRVKFNQVFGHVFEVPGSARAKIPEDALKRQTLASVERYATA
                     ELVALDEKLRSADEKLAEREGEIFRELCAAATAESAAVTALSGRLALLDVLVSFARVA
                     RAESWCRPRLVAEPGLSIVDGRHPVVEALRRREPFVPNDTELGGDARVVILTGPNMGG
                     KSTYLRQNALAVLLAHAGAFVPAASATVGLCDRIFTRVGASDSLARGESTFLTEMAET
                     AHILRHATRRSLVVLDEIGRGTATFDGLSLAWAIAEHLHDTPGAPARVLFATHYHELT
                     ELALVREGVKNRTMSVKEWKGEVVFLRKVVDGTADRSYGVQVAKLAGIPAAVTERAKE
                     ILRNLERQQLDVGGRPRLAEHEGAPAPSETQLDLFRGQEEVVVEALRRVDVESLTPLA
                     ALNLLASLRARLEGLE"
     gene            21900..22496
                     /gene="tsaB"
                     /locus_tag="PLP50_04030"
     CDS             21900..22496
                     /gene="tsaB"
                     /locus_tag="PLP50_04030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPA50749.1"
                     /translation="MRLLAIDTATPRPSLALVVDGSEPWTVELPQKGAEALAPAISSL
                     LVSAGASPRDLDRIAVVSGPGSFTGLRSSLAFARGLARAAGALLVPLPTFEVVSAAVP
                     EPPDADLLLDALRGEVHRRRRRKGHLAPAEDRVARSEALEDAVRDGVPVLDVDAVAVL
                     LAPAAAGLAANAAPDGTAGLLYGRPPAAEERFGPGGAG"
     gene            22493..22969
                     /gene="rimI"
                     /locus_tag="PLP50_04035"
     CDS             22493..22969
                     /gene="rimI"
                     /locus_tag="PLP50_04035"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPA50750.1"
                     /translation="MSALPAELTVRDVRNVDLDAIAELERVSFPVPWKREFFAAEVGT
                     AHRFNRVVRAPGGLLAGYVFCAFAGGEVHVNKIAVDPSCRRRGIARVLMQEVFDLSER
                     IRAEEIYLEVRPSNVPARNFYSSLGFREVGRRPQYYADGEDALVMSLFLTPRPASP"
     gene            23035..23274
                     /locus_tag="PLP50_04040"
     CDS             23035..23274
                     /locus_tag="PLP50_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HPA50751.1"
                     /translation="MPTLTEELKRELAQTHDEFKNLVRTHHDFEKRLAELASKTFLSS
                     DEEGEEKRLKKQKLIVKDRMEEIAREHRARVGAPH"
     gene            23317..24081
                     /gene="pssA"
                     /locus_tag="PLP50_04045"
     CDS             23317..24081
                     /gene="pssA"
                     /locus_tag="PLP50_04045"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPA50752.1"
                     /translation="MNGTSSEVQRRGVRRGVYVIPALFTVGNVFCGYLSLMAAVRGRY
                     DLAAGLVFLAGFLDSLDGRVARMTGSTSAFGEQLDSLSDVLSFGLAPAFLVFHWGLSA
                     HGRLGLFASFLFLVCGACRLARFNVQIHVVDKRWFVGLPIPAAAGALCGLIWVIPEPL
                     ADPRIATAFLGVTLLLGFLMVSTFRYRSFKDVDLRSRRSMRLVPLLGLVIAAVAAWRP
                     DVCLASLAVVYALSAPVARLASALFPRRTPLEDGPA"
     gene            24078..25118
                     /locus_tag="PLP50_04050"
     CDS             24078..25118
                     /locus_tag="PLP50_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50753.1"
                     /translation="MIGLSVALVDPGSLAGRDVKAVLRERSFPSVRLHLFHTGDSTSG
                     LLTEDDGEAALASPLEPDGLEGCQIAFLCGPPDATARFLSRRSKDGCLAIDVSGQRGA
                     GTRVDALRGVLPSPLPESDLLVLRDPTATVLADAIVAAESTGALAGVTAAIDRPASDL
                     GKDALDELFQQAIAIASFRSIPKDRLGAQCAFNVYSPADTDAFDGRVAEDVRELLGRD
                     LPVTTSPRGRASSTGPRFAWSSVSRGRPLRPARCGPRSCRPATGLPRPTSTAPPGPST
                     PRAGTRRFCSGPGPPARRAGSSSRPTPSGAPAPSWPFGSPRPRSPTAASSPTPDGGDR
                     RLQRFFSRGRGG"
     gene            complement(25085..26629)
                     /locus_tag="PLP50_04055"
     CDS             complement(25085..26629)
                     /locus_tag="PLP50_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50754.1"
                     /translation="MSRSKLHAHRAAIAVLLLGAFASAGCRKDAPSGGRGPSSAAARV
                     DALFLAEAPVVVPEEIAPELDEMGVRRLYVAAASLSAGGQVRSFPPPPARVPRPVLLV
                     LMGEPGVEGGLRGNAPEALGEAWASGTTRLVAEAKGWAEVIGVHVHLVPTAGDAAALA
                     AALGALKSRLPGLTLSVTLRAGAPEGTWKPLAGSVDEALVLTYGRRPETADAFVSEMT
                     EEEAKAFPIPFRLLAIPGSYGVAGSGARARRLPDGEMDKLSEDRNLDFDFASVLSSDP
                     GNIYSFKPRLGYEKATNLLSEDGGRARFDVLPFADLVRLVSATSRWSVPRLSGRVFLV
                     DGVPRDGRLSGYPAVRALLTGKPWEPSLRVEAIEEKTRGGEVWLRVSNPGPTPSELSR
                     FGNQITIRLEGGVFTGLGAGDFDRYEIFASARDLTQSAPFGRATACRLFENFFAPEES
                     NELGPIRIAGKSPRMFVTYQLTLPDGRTIDGAEAEVAIPAPPSPKAAPRTPAKRQPPL
                     PRLKKR"
     gene            complement(26645..30538)
                     /locus_tag="PLP50_04060"
     CDS             complement(26645..30538)
                     /locus_tag="PLP50_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50755.1"
                     /translation="MTLRRAAALLLGLLAAGGLASALSARLAPLPAGAEERREDRLVE
                     RARRALESDARRLADVAGKLASDAALTEIVEGGTAGIRPGGVYRLLGAALPEGPGWGI
                     VLLDRSGEAVAWAGEPGDLPDGAAPRAGPFAATFRVTEGTLSHESRLGRSPETQGYLI
                     VTRRFPTGIVRPDLLDANRGPDGATQRRVRVRAARSPDRLFAVSVEPGPRELEEEDVR
                     TSSSRVGALLCGVAAVGLGLLARRPATGAVAARLVLLLAVPHADGGPFARILADPTGL
                     LATPFDAAFTGFVSLVLLRSALDAPRRPQRAAGRLAALVAAVLAASAPAVLGLAGGLA
                     VPTLLLGLGLFTGPLEAPLTSAGFVAVATSLVGLGALLAGQALRPGRGRAAVGVIGLG
                     LVVWCAFASPSVASTVPLLAGAVLLVVGLSGPAARFAGADLLSKAVAVALLVGAGALV
                     TGAGAALGRLSRAESRLEAAASRSMLERERLEEEGPAAWEERIASPDLLPWTPAGSRT
                     VMSDLARALWLRGRNAEFPERGDLLTVRDEQGRVLSSFGTMRPGDEGRGTAIGLTLPV
                     PDTLGVLSRIPWPDADEEDPLLVGELGNPVHADRPVERIDFDGAGRPLGRGRLERSEL
                     AGPLLAEVRRGGAGWLTEESQGRRWRMSVRGTGGGFVAWGVEVPTPVFTLVSAVAAAE
                     TALPIALLLLLRRKLAALARARLARSGVRRGARLFGTFRGRLALAFLVSGAVPLALGA
                     ALVRTALDRSTARATEQHALELLREGRRILEDRVAGSPSPTDLNQAASVVGVDLLLYR
                     EGRLAAASRAVPVAAGLAPEHLPSALAATLADGASSAARTRAPLRPGGRRVAQAAVTL
                     SREDRTTLAVVLGEDPAGRAAVDALFLLAAAVALGALALGGRGALALSRPVEELVAAS
                     ERIGAGEAPGPIRPPEMEDLARLVEAFGTMSERVRDRTELLEREREAAVSVLASLTPA
                     ALLFREESGRILYANPAADALLPGGTTLPERLPEPRYAPVLAALRSPRPFEARVTLHG
                     EGRETVLRVVVADLTADSSGPRAVLLLEDLTDFTRAERLEAWVEAARAVAHDIKNPLT
                     PIRLTAERLLRGAGRGTPPDGARVAEAAETILRQVELLTERTGRLSRFASPAAPERAR
                     LSGESVRGLLREVAADFGAHSAVKVGWEVDGELPDVFADRALLRDAVSNFVLNAVEAL
                     EPRGGVVVVGARPASGPRGGAGLEIACEDDGPGVAEADLARLFDPTFSTKSRGSGMGL
                     AAARRAVEEHGGRLFAERSVRGGLRIGFVLPAAGR"
     gene            complement(30535..32217)
                     /gene="hutU"
                     /locus_tag="PLP50_04065"
     CDS             complement(30535..32217)
                     /gene="hutU"
                     /locus_tag="PLP50_04065"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPA50756.1"
                     /translation="MSTVSAPARYAPPALPRGTSLRCRSWLTEAPYRMLLNNLDPDVA
                     ERREDLVVYGGSGKAARNPECLEAILRELERLGDEETLLVQSGKPVAVFRTHADAPRV
                     LIANSNLVPKWGTWDEFRRLEALGLTMYGQMTAGSWIYIGTQGILQGTYETFAEAGRR
                     DLGSPDGSLAGKLAVTAGLGGMGGAQPLAVTMGGGTALVAEVDAHRIDRRLETRYCDE
                     RIDDLDAAIDRALAARDAKEGISVGVLANAVDLLARLVERGIVPDLLTDQTSAHDELN
                     GYVPHGLPYEAALALRSSDPEEYRRRSVASMGEHVRLMLELQRRGAVTFDYGNNIRAQ
                     AVKAGIENAFEIEGFVPKYIRPLFCVGKGPFRWAALSGDPADIAATDRALVELFPENA
                     ALHRWLDMAAKRVAYQGLPARICWLGYGEREKAGLAFNELVKSGKVKAPIVIGRDHLD
                     CGSVASPNRETESMKDGSDAIADWPLLNALVNTAAGATWVSIHHGGGVGIGYSMHAGM
                     VVVADGTDAAARRLSRVLVSDPGMGVVRHVDAGYPEAVACADAQGVAIPMRR"
     gene            32345..33052
                     /locus_tag="PLP50_04070"
     CDS             32345..33052
                     /locus_tag="PLP50_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPA50757.1"
                     /translation="MTSHHREIVRYRNRKLYEKSERRFVTLDDIARAVAGGDRIRVVS
                     ADTGEDITARILSRALASDRVAFPASTDALALLLRAGSDAAETVAGVAEKVGAARMAG
                     AVRKAAQPERIAETLAPVTRRVETARHDVERIVAGLVGRGRLTWEEGARLREDVGTVF
                     RESLSDVVGRVRDVVRKVSPASSPAIHAEIQELARRLDHLEALATASFPKGSSAAPRN
                     DHAARPAPARRKETRKP"
     gene            33049..33534
                     /locus_tag="PLP50_04075"
     CDS             33049..33534
                     /locus_tag="PLP50_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HPA50758.1"
                     /translation="MTRKTTRRAAPAAKSLPFDVPPQVDRALRSAVEKVDAVRDETRT
                     VASKVEKTVRAAATSAKKTGETLARDPKRFVEGVVRDAKSNLKTVGADVSREAGRFAD
                     EVARRVSGVVEPALEKALHRLNVPTQKDLKALVAKVDALGRKMEQLHAPARKPRTRAR
                     A"
     gene            33610..34788
                     /locus_tag="PLP50_04080"
     CDS             33610..34788
                     /locus_tag="PLP50_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPA50759.1"
                     /translation="MAAPRLRLVSSRGPSSPRPLPSRRGLVCAGGGITGAIYEIGVLA
                     ALEDRLDGFSLTECDVFVGVSAGAYVGALVANGTSPSVLYRNATHPRGEGLDRLSFFR
                     PNLGEIVSRAVSAPRIVFEALRDLRESPGDLTLTDLVASLAGLLPSGFFLLDGLEAWL
                     SEWLSQPGRTNDFRTLRRDLRLVAVALDSGETRVFGASAGETVPISRAVAASCALPGF
                     FRPVRIDGVDYIDGGVRKTAHISLALKDRCGLVLCVNPIVPLRFPLSPALSPRHPGQG
                     ALAGRGLLSVLDQVFRLSLHSRLRYGLDRYRREYPDADIVLFEPKAEELPRLMRNMMR
                     TSGRVRIAEYAYRTTQQKLDAEYGRLSRVFERHGLRLRPATSVRRALRRRTAAQSSAS
                     "
     gene            complement(34796..35770)
                     /locus_tag="PLP50_04085"
     CDS             complement(34796..35770)
                     /locus_tag="PLP50_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA50760.1"
                     /translation="MSTRPAAAHAEPAKPFAPSLVPAAPDAKLLRELAGEAGKLLVAH
                     PELASPRTWIDLWNLFLHRNQSLELDDFGWDEAAARVWHPLFDFLYRVWWRVTLTGVE
                     NVPNEGRALLVINHSGVLPWDGAMLKHGIAHEHPARRQARMLALDMFTTLPFLQPWLR
                     QMGEVRACPENGERLLERDELVAVFPEGVKGVGKYFRDRYRVARFGRGGFVRLALKTR
                     SPIIPVSVVGAEEIYPMLLNLAFVGRPLGFPYFPVTPTFPLLGPLGLVPAPTKWWIDV
                     GAPIDAGLDPAYAERPMIVNEVTDLVRSTIQRMIDSRLARRGNVFTGE"
     gene            complement(35767..36954)
                     /locus_tag="PLP50_04090"
     CDS             complement(35767..36954)
                     /locus_tag="PLP50_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA50761.1"
                     /translation="MTGPERAGGPRPRRRPGGEVVGFPPAAGARPKVAVTGGSGYLGA
                     RFVRALAARGTWDVVVLDLAPAANAPPEVRHRFLDLNLPHSDGTVFKILKEERPDVLV
                     HLAGLRSPLRDEVYSHELKSIGTLNVFGAAGEAGVRRLVHYSTTMVYGARGDNPAFLS
                     EEHPMRADVSDRFVRDSVEAERHCRDFARRHPDAKVAVLRFAPILAAERRDYRIRYLE
                     GPVALTMLGYDPLLQWLHPDDASEALLRTVDTPGAHGVFNVAPDGVLPLSSTLLLFGT
                     VSVPLPHALAYTAAEAAWLAGLATAPGAHAHYVRFPCVADNGKARRVLGFSPKRTTLE
                     TVVEAARTRRGRGRAIDFDAVEEVVRAATWRLEQRLRRPAPAASGGEPERPARPAGTR
                     RTA"
     gene            complement(36969..37256)
                     /locus_tag="PLP50_04095"
     CDS             complement(36969..37256)
                     /locus_tag="PLP50_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50762.1"
                     /translation="MAETLIRQLLGRGEEVVTEVTNRLLSNEAFVEMLKRGIAVKEVV
                     DGQVAVALKRMNVATRRDLARMEARIAALEAELESSKKAPVRRKPVRRKAG"
     gene            37379..39229
                     /locus_tag="PLP50_04100"
     CDS             37379..39229
                     /locus_tag="PLP50_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HPA50763.1"
                     /translation="MAPAEPLDGLTGARLLFVSGKGGVGKTTVASALAARQAASGWRV
                     LLLSTDGRGDAAALFGRADRGYEETELAPRLRTVTAEFDALLGDFVRTIAPVGFVASR
                     ILASPTFRYFTRATPGLPDLLLLGKLRELLAPKKPRFDLVVVDAPATGHALSLLSMPR
                     TVLRVVPAGPIHRIAGDLDTLFADGRSAALVVVAEPAELAAREAEELVAEAGARAGLR
                     AGLLVVNRVGRSGRAGTLPRGELPTIRVPELDVPRGATESPFALDEAFFGSFRRAFEG
                     SPPPRRRGPSPPPPGPGDLDLDEALREEPLVVLAGPGGVGKTTLAAAVGIASARAGQR
                     TLVLTVDPARRLAQALGLEGRLDRPVRVDLPGAAALSVLQIDPRATFDRLLARVAPPA
                     TLARIRSNRLYDGLVDALPGVVEYMGVEALAEHAGDPGIDRIVLDTAPAARGVDFLKS
                     PERMVELLEHDALRWFLRSDSLLSKALSGASRGAAVVLHLADRHLGFSFLADLAEFFR
                     AFDGLYDGFRERSVRIRALLDDARYLVVSSADRSALRTAAELAELLPAGPGKAGLLLN
                     RVSPRFGLPPLPPPLRSLPRRNFVEDAGSAESLPFRLADQLVSAEVASPS"
     gene            complement(39230..40762)
                     /gene="hutH"
                     /locus_tag="PLP50_04105"
     CDS             complement(39230..40762)
                     /gene="hutH"
                     /locus_tag="PLP50_04105"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPA50764.1"
                     /translation="MSEPVTLTGESLTLDDLVSVAWRGRAAVLSEEAARRIDASRAVI
                     DRAVREDRVVYGVTTGFGKFSDVVIPREKLRELQRNLVESHAAGVGELLPTPAVRALM
                     LLRANCLARGFSGVRRETVELLLAMLAAGIHPRVPSQGSVGASGDLAPLAHLARAVMG
                     EGEVEHRGSVVPAAEALAAAGLVPAVFEAKEGLALINGTQLTTSVGGLALARLLSLVK
                     AADLVTALTTDALKGTDAAFDPRIHAARPHPGQAASARNLRALLAGSPLRESHRHCGT
                     VQDAYALRCAPQVHGTVRDACAHALRVVTTEMNSATDNPMVFATGPDGEGELISGGNF
                     HAAPVALVFDLLTAAATDLASVSERRTERLVNPTLSGDLPAFLVKDGGLHSGFMMIHV
                     TAAALVSECKANSFPASVDSIPTSAGKEDHVSMGPIAARKLARNVASLENVLAIELLA
                     AAQALDLRRPLRSSPPLEELHARLRARVAPWDADRYAAADMDAARDVLVAGLDDLLAR
                     LE"
     gene            40819..42147
                     /locus_tag="PLP50_04110"
     CDS             40819..42147
                     /locus_tag="PLP50_04110"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPA50765.1"
                     /translation="MNTSRNAVVIVGGQWGDEGKGKVVDLISPAFDVVARYQGGHNAG
                     HTVKFEDRHFALRLIPSGICRKGVLNVIGNGLVVDPRALVAEITSLRAAGVEIGENLK
                     LSDRAHVILPTHALLDGARENALGGANIGTTSRGIGPAYESKANRSGIRVADLVDPDR
                     FVSLARPLLGHHAAVLSHFYGVEAPSVDETIETYLACGRDLAAHVADTRALLHDRLAA
                     GARLLCEGAQGVMLDVDHGTYPFVTSSSCGPGGACTGLGIPPSAIGAVVGIMKAYTTR
                     VGSGPFPTELFDETGERIRNRGNEFGTVTGRPRRVGWFDAVVARTSLKVGGLDAIALT
                     KMDVLDEHDEVRICVAYEIDGRTMTEVPARSDHYEAARPVYRTYPGWKRSIVGLDDVS
                     RLPEAARAYVAALEETVGAPITLLSTGPRREETIIRPGTVLDTWLSPARR"
     gene            42176..42757
                     /locus_tag="PLP50_04115"
     CDS             42176..42757
                     /locus_tag="PLP50_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPA50766.1"
                     /translation="MTIAGVLLAAGASRRFGGEKLLAPLGGSWVGIHAARVLRPAVDR
                     AVAVVRPGDERLADLLASEGFETLPFTGAARGMGASLAFGVASSSKADGWLVALADMP
                     FLEAATVARVAERLRLGIDRIVAPSFGGRRGHPVGFRRDLLPELLALDGDRGARTLLA
                     EHATEVLLLECDDEGILLDVDSTRDLDRDRSSE"
     gene            43004..44539
                     /locus_tag="PLP50_04120"
     CDS             43004..44539
                     /locus_tag="PLP50_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HPA50767.1"
                     /translation="MSQLDSRIAALAAQYLSLAKETLAEAIRIPADYVDKPLEKGGDP
                     LCGTSNHEQPRLEYLRKKILEIGAVRRPEDVFFDGFGNLVWQVDDPNDGIPAAQKKVV
                     YIDGHTDTVKPLRAQWKERVVGADCFDGLVDAAKLDKAFLKKELGWTPPDAEWGHLLF
                     GRGSADQLGGVVSAIIATKILVELTGEGALAGAIVRSYGTVCEEDNDGAGPMYLNREV
                     FPTAGPEVIPDVVILTDGTGCSKRGALGIYRGQRGRMQIEVTVTGKSCHGSMPWEGKN
                     PLEYGGAIVKEAAEAYEKRDGFLDDPFLGHGTRTASWARLDTPSDCAVPEKLVFRFDR
                     RLTIGETPDQAVADVERLSSVAAAREAGLSVEVTIPTYTDASWKGYALHNPQVYLGWV
                     TPEEHPSIRAAVETYKSVVSPHVDGKIGEGGVLTKEPRVERWVFSTDGVGFPVPKEAK
                     IPGAAGKAWVESGPYKYPAMFGFGTGIEQNTHKIGECLDTRELIHASAFLARFPSVFV
                     ATK"
     gene            44610..47924
                     /gene="ygfK"
                     /locus_tag="PLP50_04125"
     CDS             44610..47924
                     /gene="ygfK"
                     /locus_tag="PLP50_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HPA50768.1"
                     /translation="MEKAFRPIPLELLARWVFGELDSRNTVLGVPKASFARPDPRFAS
                     RLFGRDLAAPLGVAAGPHSQLAQNIVAAWLSGARFVELKTVQVLDELRVSRPCIDSAD
                     ETFNCEWSQELKLEQSFDEYLKAWVLVHALAAKLSLPSPGTLFAMSVGYELEGIRSER
                     VQEFVASIRNAKDALPAAVDAVARAWPGVRDVEVPSSISDHVTLSTMHGCPPAEIEKI
                     ALYLLQELGVHTWVKLNPTLLGPERLRGLLNETLGYGIEVPDAAFGHDPRFEDALPMV
                     KRLAAAARAVGREFGVKLSNTLEVVNHRPVFPAGEKMMYLSGRALHPLTLTLAKLVDD
                     ELDGSVPISFCGGADAWSFADLVADGLTPVTVCTDLLKPGGYARLAQYLENLGAAMDA
                     AGASDLDAFVARTSGGTGRRGNLARHAGRVAAERRFARRERPLAFKGERPLGHFDCIA
                     APCVEACPAHQNIPDYLWLVARGRETEAIEVIRRSNALPGVTGSICDHPCTERCVRNL
                     YDAPLGIREIKRFAFENGVSKPEVAGAPIGVKVAIVGAGPAGLSAALNLARAGIASEI
                     FEAKGRVGGMVGGAVPRYRLSDEKLAVDLDRLRSLGVAVHLNAAVGRDVSLDDLRARF
                     DYVFLGAGAQKGKKLGISGEDAEGVLDALDFLDGLREGVKRDLGRRVLVVGGGNSAMD
                     GARSAKRLVGRDGEVSIVYRRTRAEMPADPEEVRDCDTEGIGLKTLLAPLRVVVENGR
                     AVGLACQPMKLGEPDASGRPRPVPVEGSEVVLPADTIVTAIGQETALELLGGAAVETR
                     RNGTIAAKPATGETSAARLYAGGDVVRGPASIIKAVADGRAAAEAIARLHGIEPVAEP
                     HLPKDLSAAAALAKKARLAAPEPVPELPVAERVGFDEVLRPLSPEAARREASRCLDCD
                     EVCSLCVTVCPNRANLAYEVSPLSIELPSFVVEGGRPVPAGTTALHVAQGIQIVNLGD
                     ACNECGNCVPFCPTSGAPWRDKPTFWLDATGFAEAKGDAFRMTRDGDALVLDARIADR
                     AHRLEHHGALAEYRSDSLRVVLEPAGWHVLDVRLEPGAVEGDRLDLAACALLVALLPA
                     ESALPPLPAGNAGGRA"
     gene            47921..49267
                     /locus_tag="PLP50_04130"
     CDS             47921..49267
                     /locus_tag="PLP50_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA50769.1"
                     /translation="MKTLVRNGRIASDGRVESADLLLADGVVAAVGHDLGPAEREIDA
                     SGCWVLPGLVDLHVHVADRIGRFELADDYESGTRVAVKNGITTLAAFVTQGPAESLGE
                     ALRRARARAAGRCHADVTWHLTPTRFEAADWAEMESLVRAGYRTFKLYTTYRGAGLFS
                     DAVRLDELFARLGPLGVRFLLHCEDDALLEAVDTSSIDLSRASSHAKLRPEAAEVSAV
                     EALLDLACRRGVPLHVVHVSTVGAAERIRDARARQDVTCETGPQYLWLDETSLDRPDG
                     HRWLCSPPLRGDRERFRALALEGAFDLFATDHCPFRKADKDDWDGRDVRTVANGLPGL
                     GGLLRHAWRLWADEPDRAAAEVSLRLSRNPALRLGLDGRKGALRPGLDADVVVLDPNG
                     PARAVRATESDAYDPFPGFTSTLALRHVLVHGEPVVRDGDLVDADARRGQLHPQHD"
     gene            49289..50482
                     /gene="ygeW"
                     /locus_tag="PLP50_04135"
     CDS             49289..50482
                     /gene="ygeW"
                     /locus_tag="PLP50_04135"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPA50770.1"
                     /translation="MPSQTIQDLVTELQGLPTDLFQKDFLLTWEHPEEQIRAILTLAE
                     TFKRLHQEGTSYRAFDTGLAISIFRDNSTRTRFSFASAASAMGLSLSDLDEQKSQIAH
                     GETVRETANMISFLTEVIGIRDDMFLGAGNTYMREVGAALTDGTKQGVLHRRPSLINL
                     QCDVDHPTQSLADLAWLKKHFGGLENLRGKKIAMTWAYSPSYGKPLSVPQGIIGLMTR
                     FGMDVSLAYPEGYGLIPEVVEVAKKNAAEAGGTFEVVNSMEAAFANADIVYPKSWAPY
                     EVMGRRTELLTKGDKDGLKALEKECLANNAKFTSWECDRRKMDLTKGGKALYMHCLPA
                     DITDVSCKAGEVSAEVFEQYRIPTYHEAAYKPFVISALMFLERIADPAAKLSEIVSRG
                     RKLHI"
     gene            50491..51948
                     /locus_tag="PLP50_04140"
     CDS             50491..51948
                     /locus_tag="PLP50_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPA50771.1"
                     /translation="MSRIVKSIDTEVRKRTAKRCRERGIVIPTYRQMRDPQLLPESAK
                     KRLEGVGLWDVNPANLFRITWKNDPVTGLFGGVNALEIPREITGVKARIVGLVGKWFP
                     TGAHKVGAAFSCLVPRLVSGEFDPEKHKAVWPSTGNYCRGGAFDSAILGCHAVAILPE
                     NMSQERFTWLRTIGSEVIATPGCESNVKEIYDKCWELKKDPVHVVFNQFDEFGNPIWH
                     YNVTGPAVEDAFQAVRGPKSRLSAWVSATGSAGTIAAGDYLKRLFPAMKTVASEALQC
                     PTLLMNGFGGHRIEGIGDKHVPWVHNVRNTDAVAAIDDEDCMRLLRLFNEPAGRELLG
                     AAGASADTVGKLDLLGISSIGNVLAAIKAAKYWELDENDVVMTVFTDSAEMYLSRLAE
                     LTAERGAFTAVDAARTFTGSLERQATDNFKELTYQDRKAIHNLKYYTWVEQQGKTFEE
                     INAQWDPEYWRELFEDEVVHFDRLIEEFNAEVGLG"
     gene            51983..53308
                     /gene="ssnA"
                     /locus_tag="PLP50_04145"
     CDS             51983..53308
                     /gene="ssnA"
                     /locus_tag="PLP50_04145"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HPA50772.1"
                     /translation="MRLLIRNGTLVTLGEPCRVLEGHDLLVEGDRIARIAPAGEIPGP
                     FDREVDARGKVVLPGLVNAHMHFYSTLVRGLGKAAPSANFQEILENLWWRLDRKLSLD
                     DVEVSARVILVEAIRRGTTTLVDHHASPGAVRGSLGRIAKAVKETGLRSCLCYEVSDR
                     DGARVTEQGLEENAAFARECAAASDPQLRALFGLHAAFTLSDETLGKAARMGRDLGVG
                     FHVHVAEAASDVAANRAKHGKSSARRLIDHGILGTGSIAAHAVHCDDADKDALAASGA
                     WVVTNPQSNLNNAVGIADVVGLVRRGVPVGLGTDAMTVAMLEELRVGLFAQHLRQENP
                     TCGFLELTGTLFVQNPRIASTLWGFPIGTLAEGAAADVILVDYLPPTPLNDATALGHL
                     VFGLSQAVVDTTICGGRVLMYGRRLEIGLDEEALAAESRRLATALWERF"
     gene            53373..54317
                     /gene="arcC"
                     /locus_tag="PLP50_04150"
     CDS             53373..54317
                     /gene="arcC"
                     /locus_tag="PLP50_04150"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPA50773.1"
                     /translation="MTRTAVIAIGGNSLITDKEHQTVPDQYQAAADTCAHIAPLVAGG
                     DLRLVITHGNGPQVGFILRRSELAAHELHMVPLDSCVADTQGALGYHIQLALGNELRR
                     IGATAGTATVVTQCLVDRTDPAFAKPNKPIGSFLTKEQAEHHRETDGWDVVEDSGRGW
                     RRVVASPVPKAILELGAVKSLLGAGFVVVAAGGGGIAVVDDGTGKLKGAAAVIDKDHA
                     SSLLARELGADLLVISTAVEKVCLNFGKPDQMDLDRMTVAEAKRYCAEGHFKPGSMLP
                     KIQAVIAFLEGGGREAIITDPAHLGEALAGKTGTHVVP"
     gene            54421..>56623
                     /gene="xdh"
                     /locus_tag="PLP50_04155"
     CDS             54421..>56623
                     /gene="xdh"
                     /locus_tag="PLP50_04155"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HPA50774.1"
                     /translation="MSTTQVERPKDAAPGGAAAASFRVNGKEVTAPRDVKLLTFLREE
                     LDLTSVKNGCSEGSCGACMVLVDGKPARSCLTTTAKVAGKSVVTVEGLTEREKDVYSW
                     AFANAGAVQCGFCMPGMVMTAKGLVDANPEPASEDVAKALQLSMCRCTGYVKIEKGIL
                     DAARLLREGTEPPDEHDVPLSVGAKVLRADAREKVVGAAKYVDDMKVPGMLHGAVLRP
                     PKARVKVLGIDATEALAMPGVVAVMTAADVPGERFQGHIVPDWPAMIAVGEETRYVGD
                     AIALVAAETREIAKEAVKRLRLDFEVLPPLVDARKALDDDAPKLHPKGNLLAKTVLKR
                     GDPDAAIASAAHVIRKTFRTPETEHAFMEPESALAVPHDDGTMTVYTGTQSIYDDHKG
                     IVGVLGVAPEKVNVVSAYVGGGFGGKEDLIVQHHAALLAWKTGAPVKVTLDRQESIRV
                     HPKRHAMEMEYVVAADAEGRVTAVKAHILADTGAYASLGGPVLQRACTHGAGPYTVPN
                     VEMVGTGVYTNNPPGGAFRGFGVTQSCFAIESCLNLLAKEVGVSPWEIRWRNAVGPGD
                     VLPNGQVCDDGTALKECLLAVKELCERRPDAGLAVALKNAGIGVGLPDVGRVRLKVEG
                     GKAVIYTSAACMGQGLASVLMQFVAEITGLGWEQVAVAPPDTSTSPNAGTTTASRQTL
                     FTGEAACRAARALKADLDSASLEALEGKEYRAEFSGVTDPFASDKPNPVSHV"
BASE COUNT         7946 a        21031 c        20089 g         7557 t
ORIGIN      
        1 acccggcgcc ggcatagagg tcgagggcac cgcgcgagac cccggcgcgg gccgcctctc
       61 gcgcggcggc ccgcgcctcc tcgaggaagg cgtcgagaag gaagcggttc gcctggaaaa
      121 acgaggagac cgagacgcgg aacggtgctc ccgcgacgac gagccggagc gacgacgggc
      181 cgcgcgtggc ggcgagtcga ccggagaaga ggacccgtag gccgtccaga ggtccgtgaa
      241 gcgccgcggc gatccgctcg ggatcgggca gggcccggtc aaatcgaagc tctccgagga
      301 gcggggaccc gtcggtcccc tcgagcgtct gaagctcgca cgccgggacg ttcgcggtgc
      361 cgagggcggc gcggatcgcc ggaaggcgct cgagcagcgc cgtcgagacg acctcgcacg
      421 cctcgaggtc cgacacccgc gaagagcccc tcccgaggaa cccgagcgcc cccccgccgt
      481 cccagcgcag ccggctccgg agcctgtagc tccggggcga cgcgacgaag cggacgtccg
      541 gcagctcgcc gtccggcacg cgcccgatcc gtcggagggc gtcgcggacg agctcctcct
      601 tcaacgcccc gtgcgattcg atgcgggcgg ccggccagtc gcagccgccg caagccccgt
      661 cccgcgcgag gggacagacc gcgtccggcg gtctccggtg cggcccgggc gcgagaacgg
      721 agacgacctc cgcacgcagg aggcgcgggc cgtcgctccg ggcctcgacg aggaggcgat
      781 cgcccggcag gccgccgtcg acgaggacga cggccccgtc gatccgggcg aggccgcttc
      841 cgcccgggac gacgcgctcc acggtgacct cgttcggcac cgctcagcct ccgagcagcg
      901 tcagcgccgg aaggccgagc cgccgccgca cggcgcgcgc gcgcagggcc cgctggatcc
      961 gcccccgcgc gtccttcgac aggccggaga gatcccccgc ctcttccgcg acctcctccg
     1021 cgagcgccgt ccgccccgcc tcgccgagcg ccttctcgac ccgcttcacc agcgccttct
     1081 cgagcccgcc caggctctcc tcgagcgact ccagcccgga ggacgggtcg agctccccga
     1141 gtgcggtccg ggccgcggcg agggcggcgc gaacgaccgc cggatcggcc tccccgggga
     1201 cgttcgcctc cgccacctcg agcgcctcgg cgagcgcggc gagacggggc gccgcgtcgg
     1261 aagcgtccgg agacgtctcg gcgcgcgccg agtgcgcccc gacgagacct cgccgttcca
     1321 tctcccacag gacctcgacg gcgttcgcgc agtaggagag gctggagatc ttcgagaggt
     1381 ctccccgcgc gcgcttcctc tcgaaagcct cacggacggc ccggatcacg gtgccggagg
     1441 gaactccgct ttcgcgccag ccgcagatga gccgccagtc cttcggcgag aggaggaacg
     1501 gcgtcccgcg ttcggcgatg aaggcctcct cgacacgcgt gccgtaggag gcctcgtcct
     1561 ccgcggcgtc aggagcgccg gggggggcgg gcgtcggcgt tccggtcatg gaggatcttc
     1621 agcccgcgga gggtcaggtc ggcgtcgacc gcggcgaggt cgggggacgc ggaggcgtag
     1681 aggggcgcga ggcccccggt ggcgacgacc ttcatccccg gctcgccgag ctcggcgcga
     1741 atccgcgcga tgagcccctc ggcctgcgcg agaacgcccc agtagagacc cgactggatg
     1801 ctctgcgccg tgttcttccc cacgaccttc tccggccggt ggaggtcgac gcgagagaga
     1861 cgggcggcgc gggagaagag ggcgtcggcg ctgatgcccg gtcccgggca gatgacgccg
     1921 ccgaggtagt cgcccgcggc ggagacgacg tcgaacgtcg tggccgttcc gaagtcgacg
     1981 acgatgcacg gcgccccgta cttcgcgacg gccgcgacgg cgttgacgat ccggtccgcg
     2041 cccacctcgg aaggctggtc gtagaggatc ttcatccccg tcttcacgcc gggcgcgacg
     2101 aagagagggt cgatcccgaa cgcgtcgcgg caggcgaccc ggatcgggaa atcctgcgga
     2161 gggacgacgg aggagacgat gacgtcctcc acctcgcccg ccttccgccc cttcccctcg
     2221 atgaccgcgc ggaggaagag cgcgtgctcg tcggaggtcc tgtcgcggac cgtggtcagc
     2281 cgggccgtgg cgacgagcgc gtctccctcg aagaagccga ggacggtgtt cgtgttcccg
     2341 acgtcgagag cgaggaggag tcccatcccc gcctcagaac cgcgtgacgt cgccggagat
     2401 cagctccgcg acgcccccgt cggcctcgag ccggagaaga ccgtccttcg tcagcccgcg
     2461 gtagcggccg gacacgaccc gctctccgtc ccggacctcg agcgggtcgc cctcccggtg
     2521 gagcgtaacc gccgcgaacg ccgcgggaag gtcctcgacc tccccctcga gcgtggcgtc
     2581 gagcgcggag atcacggcga cgagcggaag gtcgcccgaa agaagcgccg gctcgacgcc
     2641 ttccagcgcg agcgacgtcg cagccgcgag cccgagcgcg tcgagctccg accgcgtcgt
     2701 ggagacgttc aggcccaccc cgacgacgac gtaacccgcc ccttcaggga gcgcccgtcc
     2761 ctcgacgagg atgccggcga gcttcttccc gcggcagaag aggtcgttcg gccacttcag
     2821 ccgcaggtcg agaccgaagg ccgccgagag cgagcgggcc aggacgatcc ccgtggcgac
     2881 cgggacgtgg atcctccccg gaccttcggg ccacggcgcg acgagcgaga ccgccacggc
     2941 gccttcgccg gtcgtcgacc agctccgccc cgcccgtcct ctccccgccg tctgccggcg
     3001 cgcagcgacg caggtcggaa ggaggtcggt gtcctcggcg accatcttct cgaccagggc
     3061 gcgcgccacg tcgttcgtcg acgccgtctc ctcgaggagg acgacgttcg cgacgcgccc
     3121 gaaacgcgct cctccgagcg gcgcgagggg tccgagcggg ctcgtcaccg cgcgccggcc
     3181 tccccgaggt tcgtcacgag cttcgcccgg cgcgcggcga gctcctcgag ctgcttcctc
     3241 gccttctcga cgaccgcctg cggcgcccgc gagaggaacg actcgtccgc gagcttccgg
     3301 ctgacgcctt cgatctcctt gtcgagggag gcgatctcct tgcgcgcctt cgcctcctcc
     3361 tccgccgaga gctccttctt cgggagcgcg acgacgaggc cggcgacgcc aacgtggtcg
     3421 cggaaggcgt tcgggaggtc gaccttcggc gcgacgacga ggctcgagag gcgcgccatg
     3481 tgggagagga gcggcgcgtg ggcgcggagg gcctcggcga cctctccctc aggcacctcg
     3541 agaccggcgt cgagcgcctc ggtctgggcc aggccgcgct cggcacggag gttgcggatg
     3601 cgggtcgccg tctcgcggag cgtctcgacg acctccacgg cgaacgggtc gcgccagtcg
     3661 tcacgtccgg ccgggtacgg caggaccggc agcttcgccc cgtcgccgtt cagcgcctcg
     3721 cggatctcgc aggagacgaa cggcatgaag gggtggagga gcgcgagcga gtcgaggagg
     3781 acgtgccgga ggacggcgcg ggtcttcgcc ttctcctcct cggggagcgt ctcgtcgcgg
     3841 aggacctgct tgaccatctc gacgtagccg tcgcagaact cggtccagaa gaaggcgtag
     3901 agggcgttcg ccgcctcgtc gaagcgaaag acctcgagcg agtcgttcac cttcttcgcg
     3961 gtcgtcgcga ggcggtctag gatccagcgg tcgacgcgac cgagggaggc gaaatcgggc
     4021 tcgctggcga cgggcgtctc gccgagcatc cccatggcga aacgggcggc gttccagacc
     4081 ttcgtggcga aggcgcgcga ggccgcgacg cgcgtcgtgc cgaacggcag gtcgcgcccg
     4141 gtgcccgaga ggacggcgag cgcgaagcgg accgcgtcgg cgccgtactc gtcgcacatg
     4201 acgagcgggt cgatgacgtt ccccttcgtc ttcgacatct tctggccgcg ctcgtcgcgg
     4261 acgaggccgt ggaggaagac ctcccggaac ggcacctttc cgtcgaacca gacgccggcc
     4321 atgatcatcc tcgcgaccca gaagaagagg atgtcgaagc cggtcaccat gacgtcggtc
     4381 gggtagaagc gggcgaggtc cttcgtcctc tccggccagc cgaggaccga gagcggcatg
     4441 agccacgagg agaaccaggt gtcgaagacg tcggggtcgc gctcgagctt cgcgctgccg
     4501 cagacggcgc acgccgcagg gtcggcctcg tcgcccggct tcggcacggt gacgtgcccc
     4561 tcggggcagt accaggccgg gatctcgtgc ccccaccaga gctggcgcga gacgcaccag
     4621 tcgcggatgt tcttcatcca ctcgtcgtac gtcttcttcc agagctccgg cgtgaacgtg
     4681 acctcgcgcg cctcggtggc ggcgaccgcc ttgtcggcga gcggcttgat cttcacgaac
     4741 cactggagcg agacgagcgg ctcgaggacg gtgtcgcagc gctggcagcg gccgacggcc
     4801 gacgtgtgcg gcttgtcgtc gcgccggagc ccctgctcgc ggaggtccgc gaggaccttc
     4861 ttccgcgcgg cggtccgctc gaggccggcg taggccgcgc ccgcctcggc ggtcagcttc
     4921 ccgtcgaagc cgatgacgac gacctgcggg aggccgtgcc ggcgccccgc ctcgaagtcg
     4981 ttcgggtcgt gcgcgggcgt gatcttcacg acgcccgtgc cgaacttccg gtcgaccatc
     5041 tcgtcggcga tcacggggat gagcctctcc gtgagcggaa ggcggacctt cttcccgacg
     5101 atcgcggtgt agcgctcgtc ctcggggtgg accgcgacgg ccgtgtcgcc gagcatcgtc
     5161 tcgggacggg tcgtcgcgac gacgacctcc ccgctcccgt cctcgagcgg gtaggcgacc
     5221 gaccagagct gccccgagct ctcgacgtgg ttcacctcga ggtcggagag gacggtcgcg
     5281 cagcgcgggc accagttcac catccggctg tcgcggtagg cgagcccctg gtggtagagc
     5341 tcgctgaaga cgcgccggac ggccttcgag cgcgccgcgt cgagcgtgta gtactcgcgc
     5401 gagaaatcgc aggagcagcc gaggcggcgg atctggttca ggatgtcgcc gcggcgggcg
     5461 tcagcccatt cgcggcagat ctcgaggaac ggctcgcgcc cgaggtcgtg gcgcgtcttc
     5521 cccgtccgct ccgtcaggtc gcgctcgacg accatctgcg tcgcgatgcc ggcgtggtcg
     5581 acgcccggga cccagaggac cgcgcggccc tgcattctcc tccaccgcgc gaggacgtcc
     5641 tccagcgtcc gcccgagggc atggccgacg tgcaggcggc ccgtgacgtt cggaggcggg
     5701 atgacgaggg tgtaagggcg cgcgtcctcg gccgcgacct ccgggcggaa ctggcccgac
     5761 tcctcctgga gccggtacca cttcatctcg aaggcctgcg ggtcgaagcg cttgtcgagc
     5821 tcggggcggg gcgcggatga cataggccgt cgagtctaaa cgacgcctcg ggacggagtt
     5881 cgcggccggc tcagcccgcc cgctcgacct cgccgatccg ctcgcggacg aggcgctccg
     5941 cgtactccgg gacgacctcc caggcgatgt cacggacgac cttgtcggag agcagctcca
     6001 cgacgcgccg ggcgatccgg tcgacgtcgg cgtcggtgag gcccccgccg ggcgccgcga
     6061 cgaccggcgc cgtcgtaacg gccgcgacgg gctcgggcac gacggccggc tctccgggcg
     6121 aggaggccgc tccggccggg atgagggcgg aaaggtcctc gaggcgcgcc gccgcggcga
     6181 tctcctcgag gtcggccgag ccgggctcgc cccgcgtcgc ggccggcgcc tcgggaacga
     6241 cgccggcgac gtcgatgtct tccggaacct cctcgccgat cagggccgcg tggcgttccc
     6301 agatctcggg ccggcgacgg acacggccgg acgcctcgaa cgcctccatg tcggcttcga
     6361 ggtcgcgggt gaccggctcg ggctcgtcgg aaaggtcgtc gccccgggcg tcgagagccg
     6421 gcgggggcgg cacctgaagc gcggggccct cctcccaggg cgcgccatcc gccacctggg
     6481 ccgcggcctc catctcgggg aacggctcga ccgcctcggc gggcgcctcg tcgccggtct
     6541 cctcgcgcga aatcgcttcg ccggtcgacc tctcctcgaa cggctccgcc ggtccggtcc
     6601 cctcctcggc cccggtctcg acgagggcgg ggcgttcggc ctccagctcg ggcgggagct
     6661 cggtctcgaa cggcgcgtcc gaagggggct cgacaggcgc cgccgcgtcc tcggccggtg
     6721 cgccctcgaa cggctcggcc tccgggacgg cgggcgacac gaggggcagc accggcgccg
     6781 tgtcgggtcc gtccggcacg gcctcgtccg tcgtcgccgg ctcgggctcg tagaaccgcg
     6841 gaggcggggg cgggacgggc ggctccggct cgtagaacgc gggaggcgga ggcggagggg
     6901 gcggcacgaa gaccacggcc gcttcgaacg ggggcggagg cggaggcgga atgtacgcgg
     6961 cgagcgggtc gtacggcggg ggcggaggcg gttcgacgag gaaagccggc tcggcgggcg
     7021 gcgcggactc ctgagtcggc tcctcgttcc acggactcgt ctccacgacc ggaggcgcgg
     7081 cctccacggc cggagccgcg accgaggcgc cgtccacgtc gaacggcgcc tcgggcggga
     7141 gcgtcgcggc gacggcggcg aacggctccg gaaggggctc cgccggctcg aagggcgcgg
     7201 gcacgtcggc gatcggcgcc tcctccgcga gctcgaaggg cgcgggggcg gcggcggcga
     7261 gctcctccgg cgtcggcagc ttcatcgcca tcgtggcgta gatcgactcc ccggccgggg
     7321 cggcggtcgc cgcctcgacg aacggaggcg gtgcagactg gtcgaggtcg aggggcgtga
     7381 ggacgagcgt cgagccggtg tcgacgggga cggcggcctc cggtggcgcg tccaggtcgg
     7441 cgagcgtgaa ggccggcggg gcgggccgct cgggagccgg aggcggggcg gactcggccg
     7501 cttcgcgggc ggcgcggccc ttcaggacga gctcccggac gaggccctgg agggcgtgcg
     7561 agtcgaacgg cttcgtgacg atggcgtcgc tgccgacgcg atcggcccgg gcgcgatcga
     7621 acggctcgaa cgtgccggtg aggaggacga ccgggacgaa agcaccgccc ggccgctgct
     7681 tcaccgcctc gcagacgtcg tatcccgtga ggccgggcat gacgacgtcg gccaggacga
     7741 tgtcgggctg gaactcgtcg agggcctgga cggccttgtc tccgctcccg acggaaagga
     7801 gacgggtctc ggcatccgag aaggtcagct cgacgacttt ctggatcgtc aggctgtcgt
     7861 ccgcgagcag gatcttgatc gacatctcga ctcctcgaac ggccgttcga cgggaccggc
     7921 aaacgtgccc gaaagtctac gtcccggagg ggggtgggtc aacgcgaagc ggtcttcgac
     7981 gcgcgctccg acgcggctct catcgccgcg aagacgcgag agacgggcat ccgtccgacc
     8041 gccgcggcga gggcctccga ggccgccgag accttctcga ggtcgatgcc ggtctcccat
     8101 cccattgcgt gcaggaggta gacgaggtcc tcggtggcca ggttccccgc cgcgcccggc
     8161 gcgtaggggc agccgcccag accgccggcg gaggaatcga agacccgcac gccttcccgc
     8221 agcccctcga cgacgttcgc gagagccgtc ccgcgggtgt cgtgcatgtg gagagcgatc
     8281 cgttcgatcg ggactccggc ctcccggacc tttccgagga cgtcggtgac ctgcgacggg
     8341 acggcgacgc cgatcgtgtc gccgagcgaa acctcctggc agcccgccgc gaagaggcgc
     8401 gcggccacga cggccgcctt cgcggcgtcg acacgcccct cgtaggggca gccgaaggcg
     8461 gtcgagacgt agccccggac gcgaaggcct tcccctctcg cccggtcgag gacgggggcg
     8521 aaccgggcga acgactcttc gaccgtggcg ttgacgtttc gaaggttgaa ggtgtcggtc
     8581 gcggccgtga agagggcgat ctcccgcgcg ccggccgcga gggcgcggcc catgccgcgc
     8641 tcgttcggca cgaggaccgg gtaccgggtc ccgggccgct tcctcacgcg ggcgaagacc
     8701 tcggccgagt cggccagctg cggaatcgcg cgtggggaga cgaacgcggt cgtctccacg
     8761 accgggaggc cggcgtcggc gagagcctcg acgaaggcga ctttcacgtc ggtcgggacg
     8821 aagcccctct cgttctggag gccgtcgcga gggccgacct ccacgaccgt gacccgtccc
     8881 gctcccgtca ttcgatctcc gcgagcgggt cacccaggtc gacgcgctcc cccgccgccc
     8941 ggaaaagctt cgcgagccgc ccgggccggg gactccggat ctcgtgctcc atcttcatcg
     9001 ccgtcaggac gaggagcggc gtccccttga cgacctcctg cccttcctcg gcgaggaccc
     9061 gcgcgacgac gccgggcatc ggggcgcgca ggtcgtgctc ttcctcctcg ctcgcgccgg
     9121 gcttctcgac gacgcgcgcg aggcgccacg tctccccccg gtgccagacc cagacgacgg
     9181 agccgtcgcg gacgacgcgc gcgccccgcg gaggctcgcc cggcccggga gcgagctcgt
     9241 ggagggtgcc ggtcgagagg ttgcgaatct tcatccgagc acccggaagg agccttcgtt
     9301 gaacgggtcg gggaacgccg cccgggccgt cgccgaccgg gcgacctgcc cgtcgccgcc
     9361 cccgaggaag cgagccgccg cggcgaggac gtcgggcggg ggatcgtggg gcggcggtac
     9421 gttcagccgg ccgaggagcg aggtgtcgag ctcgccccgg acgaactcgg gcgtgcccag
     9481 caggcggcgg agccacgaga cgttcgtcgc gaccccgagg atcaccgtcc gcgaaagggc
     9541 gtcgacgagg cgaaggcgcg cttcctcgcg cgtccgtccg cgggcgacga cctttgcgag
     9601 catcgggtcg tagtggacgg agacctcgct tcccgcctcg atccccgagt cgacgcggac
     9661 gccgggtccg accggctcct cgtagacgag gacggtcccg acctgcggga ggaaaccggc
     9721 ctccggatcc tccgcgtaga cgcgcgcctc gatcgcgtgg gccaccggag ccgccggcac
     9781 gtcggccggc agcggctccc ccgaggcgac cgcgagctgg agcgcgacca ggtcgaggcc
     9841 ccaggcctcc tcggtcaccg ggtgctccac ctggagacgc gtgttcatct cgaggaagaa
     9901 gaaggagccg tccgcgtcga ggaggaactc gaccgtgccg gcgttcacgt agccgacggc
     9961 cttcgccgcg gcgaccgccg cctcgctcat gcgcgcacgg agctccggag agacggcggg
    10021 agacgggctc tcctcgacga ccttctggtg ccggcgctgg agcgagcact cgcgctcccc
    10081 cagagcgacg acgcgaccgt gcgcgtcgcc gaagacctgg atctcgacgt gccgcgggtg
    10141 ctcgaggtag cgctcggcgt agagcgtcgg gtcgccgaac gcggcctccg cctcggcgcg
    10201 gcaggaggcg acggcggacg cgagctcccc ttcggcgcgg acgacgcgca tccccttccc
    10261 gccgcctccg gcggaggcct tcacgacgag cgggaaaccg accttcgcga tcccctccgc
    10321 ggacaggtcg tgcgtccccg ggacgaccgg gaccccggcc gctctcatcc gctcgcgaga
    10381 cgggaccttc agccccatcg cctcgatcgc ctccggcggc gggccgatcc agacgagccc
    10441 ggccccgatc acggctcgcg cgaatgcggc gttctcggag aggaagccga agccggggtg
    10501 gatcgcctcg gcgcccgtgg cgcgggcggc gtcgaggacc ttcgcggcga cgagatacga
    10561 ctcccgcgcg ggggccggcc cgaggacgac gacctcgtcg gccaggcggg cgtgtcgcga
    10621 gccgacgtcg gcctccgaga cgacggcgac cgtctcgtac ccgagcgttc ggcaggcggt
    10681 caggacgcgc accgcgatct ccccgcgatt ggcgacgagg accttccgga gcgggcggac
    10741 gttcacgagc cgctcctccg ttcctgatcc cgggtccacg ccggggcccg cttcgagagg
    10801 aacgcattca ttccctcctg cccttccgcc gaaacgcgcc gcgccgcgat gacccccgcc
    10861 gcctccgcga cggcctcgtc gagcgggacg cgccccatcc gcatcgcgag gcccttcgag
    10921 acggcgatcg cctccggccc gccgaggagg agggcgtcga tgacgcgctc cacctcggcg
    10981 tcgagctccg cgtcggcgac gacggcgtgg acgagccccg cgcgaagcgc ctcctcggcg
    11041 ccgaaccgct cgcccgtcag gaaccagcgg cgcgcgtgcg actcgccgac cttgcggacg
    11101 acgaagggcg agatgacggc cgggacgatc ccgagccgga cctcggtcgt cccgaggagc
    11161 gtcccctccg cgcagacggc cacgtcgcag acggccacga ggcccgtgcc cccgccgagc
    11221 gcggccccgt ggatgcggcc gacgaccggc ttcgggcagg agtcgatcgc gaggaacatt
    11281 cggccgagcg cctcggcgtc gcgccggttc tcctcctcgc cccaggccac catcctcttc
    11341 atccaggtca ggtcggcgcc ggcgcaaaag gacttcccct cgcccgcgag gacgacgacg
    11401 cgtgtcgagg cgtccgacgc gagctcctcg aacgccgcgg tgacctccgc aatcaacgca
    11461 tcgtcgaacg cattccggag ctcgggcctc gcgagaacga cgcggacgac accggcgcgc
    11521 gtgcgctcca ggcggagcga cgcgctcaac gcaggacctc gatcgtcccg gacgccgcga
    11581 tcgggagctt cacgtcgccc gtgccgagct tcaggacgat ctcaccggtc agccgtcccg
    11641 agagcctccc tcccgaggtc accgccttcc ccgcggcggc gagcgcggcg gcgttcgaga
    11701 ccgtcagcgg gagggcgatc tcgttctcgc gagacgggcg gacgatgact ccctgccggc
    11761 tcccgcgcgc gaccacgcgg ccgcccgagg ccagctcgta gcggaggccc cggagggaga
    11821 cgtcgaatcg gaacgggttg aagaccgcca ggcgggcctc tcccttcgtc tcggccaggc
    11881 cgaggcccga gatgcgagag ccggcgaacc ggacgaaacc gtcggcgagc tcgcggtgcg
    11941 cgacgaggtc cgccgtgccg ggccggagcg cgccggcggc gtcgacggtc gcggccgtcc
    12001 cggcctcgcc ggcgagggtc cccttcagcg tgaccgggag ggtgacgggg ctcccccgct
    12061 cgacgatcgc cttcggcacc gccgcgaggt cgatctcggc caggaagacc acgtcgaacc
    12121 cgttggccga aggttgcacg gcgaccttca ccggctggac gagcggaacc ggagcgcccc
    12181 aggccgtcgt ctcgccccgg aatttctgcg tcgccccgcg cgccgcgcgc gggagcgtcg
    12241 tccgcacgaa gaggaaggcc ttctgccgcg tgagcgccgt gacctcgatc gaccggatcg
    12301 tccccttcgg cggcgcggcg gccgaggccg ggagggacgc gaggagcgac gcgaggagga
    12361 tccgaaggaa ccgacggcgg ttcacgtcgc gcctcacatc cggaagacgc cgaacgacgt
    12421 cttcgcgatc ggggcgttgt gacagacgga gagggcgagg gagaggacct ttcgggtctc
    12481 ggccgggtcg aggatcccgt cgtcccagag ccgggccgtg gcgtagtagg ggctcccctc
    12541 ggactcgtac ttctcgaggg tcggtcgctt gaacacggcc tcttcctccg gggacatcgg
    12601 cggctgtccc tgacgttggc gttgctcgtt cttgacggtc gagaggaccc cggcggcctg
    12661 ctcgcccccc atcacgctga tcctcgagcc gggccagctg aagaggaacc gcggctggta
    12721 ggcgcgcccg cacatgccgt agttgccggc gccgaaggag ccgccgacga ggagcgtcat
    12781 cttcgggacg gcggcgttcg cgacggcgtg gaccatcttc gcgccgtcct tggcgatgcc
    12841 gccgtgctcg tacgcctttc cgaccatgaa gccggtgatg ttctggacga aaaggagcgg
    12901 cacgcgccgc tggcaggcca tctcgatgaa gtgggtcccc ttgagagccg actcggagaa
    12961 gaggaccccg ttgttcgcga ggacgccgac cggcattccg tgcagccggc cgaagccgca
    13021 gacgagcgtc gacccgtagc gcggcttgaa ctcgtggagc cgcgagccgt cgaggaggcg
    13081 cgccaggacc tcgcggatct cgagcggcct cttcaggtcg cgcggcagga ggccgtagag
    13141 ctcgtcgggc gcgtagaggg gctcctcggg ctggacgacg tcgccggaga cggtcttgat
    13201 cgtgttcagg cgcgcgacga tctcccggac catctcgagc gcctcggcgt ccgaagaggc
    13261 gaggaagtcg gccacgcccg agatccgcgt gtggacgtct cccccgccga gctcctcggc
    13321 cgtgacggac tctcccgtcg ccgccgcgac gagcgggggg ccggcgagga agatcgtccc
    13381 ctgccccttg acgatgacga cctcgtccga catcgccggg acgtaggcgc cgccggccgt
    13441 gcaggagccg aggacggcgg agatctgcgg aatcccggcc gccgacatcc gggcctggtt
    13501 gtagaagatc cgtccgaagt ggtcccggtc ggggaagacc tcggcctgga gcggcaggaa
    13561 cgcgccgccc gagtcgacga ggtagacgca cgggaggtga ttctcgagcg cgacctcctg
    13621 ggcgcggagg tgcttcttca cggtgatcgg gaagtaggtc ccccccttca ccgacgggtc
    13681 gttcgcgacc accatgacgt cccggcctga gacgcgcccg atccccgtga cgaggccggc
    13741 gccaggcgcc tggccgtcgt acattccgtc cgcggccagc ggcgagagct cgaggaaggc
    13801 cgtctcgcga tcgaggagcc tctcgatcct cccgcgaacg gagagcttcc cgagctccgc
    13861 ctggcgtgcg gcgccccgcg tgccgccccc ttcgcgcgcc gtggcgacgc gctcacgcag
    13921 ctcgcgcgcg aggccttcgt tgtggacacg gttctgaagg tagtcctcgg acgtggaatc
    13981 gacccggctg acgagaacgt cgctcaccgg gcgattctag cggccggttc ccgggacccg
    14041 ggcgcgcctt ctccgcgcgc cccggcgtcc cccgcggtca gcggaccgct gcggcctcgt
    14101 gcggtcgcgc gaggtcccgg accgaatcga tgaccgccgt ggtcgtcgtc ccgggcggga
    14161 agaccgcctt cacgccggcg gccgtgaggc ccgggatgtc cttctcggga atgatcccgc
    14221 cgacgacgac cgagacgccc tcggcgccgc gccgcccgag cgcggagatg acctcgggaa
    14281 cgaggacgtt gtgcgcgccg gagaggatgg aaaggccgac gacgtcgacg tcttcctgaa
    14341 cggctgcggc ggccacctgc tcgggggtct gccggaggcc tgtgtagatg acttccatcc
    14401 cggcgtcgcg cagggcgcgc gcgacgacct tcgcgccgcg gtcgtggccg tcgaggccgg
    14461 gcttggcgat gaggactcga agtggtcttc ccgtcatggc ggttgaattc taaccggcct
    14521 tgggactggt tcctgctaga ttctccccat gcggcgccgg atcggggacg tcctcgtcgg
    14581 gggaaaagcc atctcccagg agctgcggga ccgggccctc gcgcttcagc tgaagcaaaa
    14641 ggggcggctc tgcacgaacc tcctggagct cggcggcgtc cgcgaggacc tgctcctgcg
    14701 cgcgctcgcc gtgcagtacg gcgtcgccac ctgcacggcc gccgacctgg ccgagatccg
    14761 tcccgacatc ctccgtctcg tcccggccaa gctcgccgcg aatctcttcg tcgtcccgtt
    14821 caagaggctc ggccgaaacc tctccctcgc catgcgagac ccgaaggacc ttccggccat
    14881 cgacgaggtc tccttcctga cgggactcgc gatcaccccg ttcgtcgcgc tcgacctccg
    14941 gatccagctc gcgctccaga agcactacgg gatcgccatc gacccgcgct acgccgacct
    15001 cgccgcccgt ctcgaagcgg gaggcggggt gctcccggtg ccgccgccgc gcacgccccg
    15061 cccgctcgtc cgctcctcgt cggccggaac gcgcgccgtc gcccccgagt cgccgcccgt
    15121 ccccgttccg gccccccccg cggcgcagcc cgcgccgctg ccggctcccg cgcccgatcc
    15181 cgctccgccg acggccccga agcccgccgc cccggccgag atcgagatgg aggcgcacga
    15241 ggaagaacgc ctgcggatca gcggcccgat cgttcttccc cccgagccga ccgacgccgt
    15301 cggcggcgag gaagtggcgc cgctcgtcgc agaggcggct ccggcgagcc tcgtcgactg
    15361 cctctcgcgc gccgaatcgc gggacgagat cgccgcggcc gtccttcgcg aggccggtcg
    15421 cctcctccgg cgcgccgccc tcttcatcgc gcaggcggag cgggtcatcg ggtgggcggc
    15481 cgtgccggag cctcccgacg ggctccggtc gttctcgatc ggcttcgccg agccctccct
    15541 cttcgcatcg ctccgaaaca ccgacggctt ctacgtcggc ccctgccccg acctgcccgg
    15601 caaccggaag atcctggagg ccctcgggtc ggactacccg gcgcacgtgg cggccgtgcc
    15661 ggtgacgctc aaggggaaga gcgtcctgtt cctcctcggc gaggttcgcc ccggcgagac
    15721 tcccccgaag gcgatggagc tgaagcggct cggagcgctg acggcgatcg cgctcgagat
    15781 ccttctcctt cggaacaagc tccgtagcct ctagcaggga cttggcttac acttcgcgcg
    15841 aatgatcaag aacgacctcg tcaaccgcgt gaccgaggag accggcgtcc cgagggtcca
    15901 ggcgtcccgg gccgtcgaga cgatcatcga ggcgatgcgc cgttcgctcg ccggaggcgg
    15961 acggatcgag cttcgcggct tcggggtctt cctcgtgaag cggcggaagc gcgggatcgg
    16021 gcgcaacccg aagcgtcccg acgagcaggt cgagatcccg cccggcttca cgatccgttt
    16081 caagcccgga aaggagctcc gcgacctgat gatcgtggag ccggcggcac ccgccgccga
    16141 gcctgcggtc ccctccaccc cggagccggc ctgagcggcc tcttcccgcc gcagctcccg
    16201 caccccgcga ccgaggaacc gggtcccgtc gtttcgtcgt ccgacccggc cgtcggcctc
    16261 ctggaccccg cgaagccagc cccgcccggt cgccgggagc gctggtggct ccacgtcctc
    16321 ctcctcgtcc tgacgttcgg gacgacgtcg tacgtcggcc ttcagctcgc cgccggatac
    16381 gacccgcgcc tcgccgtcgt cgacccgcgc tccggggtcc tcggcatcac gcccgagctc
    16441 gtcctcgccg gactcgccta ctccgtcccg ctgctcctca tcctcctggc ccacgagctg
    16501 gggcactacc tcgcctgccg gaagtacggc gtcgacgcct cccccccgta cttcctcccg
    16561 ttccttccga tcgtccccct tccggggacg ttcggcgcct tcatccgcgt ccgcgagccg
    16621 atccgcgaga agaaagtcct gttcgacatg gcggtcgcgg gcccgatcgc cgggttcctg
    16681 gtcgccctcc cgattgcggc ctggggcatc tggcacacgc gcgtggattt cgacccgccc
    16741 gccgagggga ccatctactt cggctatccg ctcgccgtga agctgctcca gctcctcctg
    16801 acggggcaca ccttttcgag cgttcacgtc gtcgagcacc cagccttcct cgccgcctgg
    16861 tggggcttcc tcgtcaccgc catcaacctg atcccggccg gacagctgga cgggggacac
    16921 gcgatctacg cggtcttcgg gagccgccac cggctgctgc gatggccggt cctcctcgcc
    16981 ctcgccggcc tcgggttcct ctatccgggg tggtggctct gggccgccat cgtcctgttc
    17041 ctgacccgcc tccggcggcc gcccgtcctc gacgaggacg tcccgctcga ccctcgccgg
    17101 aaggcggtcg cggcgggcgt cgcccttctc ctcgtgctga gcttcgtccc ggtgccgttc
    17161 cgcgagctag atgacgtcgc gccgcttcgc gcgccagtgg aacgtggcgg gaccgtcgtt
    17221 cacgagctcg acctccatcg cggcgccgaa gcttccggtc gcgaccggga ctccggaggc
    17281 gcgaagcgcc gcgaggtagc ggtcgaagag ggggcgcgcc tcctccggcc gggcagctcc
    17341 ctcgaagccg ggccgccgac cgcgtgagag gtccgcgccg agcgtgaact gggagaccgc
    17401 gaggacggcg ccgccgacct gcgtgacgtc gaggttcatc ttccccttct cgtcctcgaa
    17461 gacgcgcagc tcgacggtct tccgcgccgc ctcgtccatc gcgtcgggcc cgtcgcccgg
    17521 ctcgacgcac ccgaagacga gaaggcctcg cccgatctct ccgacggctt cgccgccgac
    17581 ccggacggcg gcgcgggaga cgcgctgaag gaggaggatc atggcgccga ttctcgcagg
    17641 gaggcggcgc agcccctctg atatcctcgc ccgccactcg atctcgaagg caggaggaac
    17701 gcccatggcg ggttccgtga acaaagtcat cctcgtcggt cacctcgggg ccgaccccga
    17761 gatgaaggcg ctcccctcgg ggatgcagat ggcgaagctg cgcgtcgcca cgtcggaaac
    17821 gtggacggac aagacgtcgg gccagcggca ggagaagacc gagtggcaca acgtcatcgt
    17881 ctacgacaag ctcgccggga tctgcgagcg gtacctctcg aaggggcagc tcgtcttcat
    17941 cgaggggtcg atccagacgc ggagctggga cgacaaggag acgggccaga agcgctacat
    18001 gaccgagatc aaggcgcgcg acatgcggat gctcggcggg cggcccgacg gcgcccgggg
    18061 cagcgccgga gcgccgtcgg cgccggagcc gccgatggac ggcgacccgg acgacagcgt
    18121 cccgttctga ggctcagaac ccgaagtcgg cgtcgtcccg acgcctccgg ggtttcgcca
    18181 ggtcctcgag ccacgactcc ccttcggccc ggagggcgtt ccacgcctcc gggccgaatc
    18241 gcgtgcgggg atgtccgtcg cagcggcgtc ggagcgcggc gatccgctcg agcgcggccg
    18301 gctcccggtc gatcctctcc gcggcgagcg cctctgccgc cgcggctgcg gcgtcgagtc
    18361 cctgcgaggc gatgaggaga tcgcagcccg agcgagcggc gcggacggcc ctctccggga
    18421 aggggatctc cgcgagcgct cccattccga ggtcgtccga gaggacgacg ccgtcccacc
    18481 cgatcctctc gcgcaggatc gcgtggatcc gcggcgagag cgacgcgggc gtctcgtctc
    18541 ccgtgaagcc gggatacgcc gcgtgcccca cgaggaccgc gtcggacgcg cggacgagct
    18601 tcgtgaacgg agcgacgtcc gtcaccatca ggtcgacgtc gtgggcgtcg acgacgggga
    18661 gcgcgccgtg agagtcgacc ggcccccgcc ccagccccgg gaagtgcttc acgcaggagg
    18721 cgaccccggc ccgggcgagc ccgtggagga agacgctccc ggcgacgacg acgtcctccg
    18781 cgtggaagcc gaacgtcctt ccggcgagga cgacggcccc cgtccccggc tgcgcgaggt
    18841 cgaggaccgg cgcgaggtcg acgtcgaagc cgaggaggcg ggccgcgcgc cccatcaggt
    18901 aggccccctc gtggactcgg tcgactccga tcgcggagag ggcggcgggc gacgggacgt
    18961 cgaagccgag gagcggcccg agccgatcga ccggcccccc ctcctggtcg acgaagagga
    19021 gcggcgaggg atcgcaggcc gcgcggacat ccgcgacgag ggcggccgcc tgctccgcgg
    19081 aagcgaggtt ctccggaaag aggaggacgc cggccggacg ccgctcggcg aggaagcgct
    19141 tctccgcgac ggacaacgcc gtcccggcga ggccgacgac gagccgggcc ccgctcattc
    19201 gagcccctcc agccgcgcgc ggagcgacgc gaggaggttg agagccgcga gcggggtgag
    19261 ggattcgacg tcgacccgac ggagcgcctc gacaacgacc tcctcctgcc cgcggaagag
    19321 gtcgagctgg gtctcggacg gggccggcgc gccctcgtgc tcggcgagac ggggccgacc
    19381 gccgacgtcg agctgctgcc tctcgaggtt ccggaggatc tccttcgccc gctccgtgac
    19441 ggccgccggg atccccgcga gcttcgcgac ctggacgccg taggagcgat cggccgtccc
    19501 gtcgacgact ttccggagga agaccacctc gcccttccac tccttcaccg acatcgtcct
    19561 gttcttcacc ccctcgcgga cgagggcgag ctcggtcagc tcgtggtagt gcgtcgcgaa
    19621 caggacccgc gccggagcgc ccggggtgtc gtggaggtgc tcggcgatcg cccaggcgag
    19681 cgagaggccg tcgaacgtcg ccgtgccccg gccgatctcg tcgaggacga cgaggctccg
    19741 gcgcgtcgcg tggcggagga tgtgcgccgt ctcggccatc tccgtcagga acgtcgactc
    19801 cccgcgcgcg agcgagtcgg aggcgccgac gcgggtgaag atccggtcgc agaggccgac
    19861 cgtcgcggag gcggccggca cgaacgcgcc ggcgtgcgcc aggaggacgg cgagcgcgtt
    19921 ctggcggagg taggtcgact tcccccccat gttcggcccg gtcaggataa cgacgcgcgc
    19981 gtcgccgccg agctccgtgt cgttcgggac gaacggttcg cggcgccgga gcgcctcgac
    20041 gaccgggtgg cgaccgtcca cgatcgagag ccccggctcg gcgacgaggc gcgggcggca
    20101 ccagctttcg gcgcgcgcca cccgcgcgaa cgagacgagg acgtcgagga gcgccagccg
    20161 gccggacagg gccgttaccg ccgccgactc cgccgtcgcg gcggcgcaga gctcccggaa
    20221 gatctccccc tcccgttccg cgagcttctc gtcggcggac cggagcttct cgtcgagggc
    20281 cacgagctcg gccgtcgcgt aacgctcgac ggaagcgagc gtctgcctct tcagcgcgtc
    20341 ctccgggatc ttcgcccgcg ccgagccggg gacctcgaag acgtgcccga agacctggtt
    20401 gaacttcacg cgaagggtcg ggatccctcg tcgcgaccgc tcctccgcct cgagggcgcc
    20461 gaggagcgtc gccgcgtccc gtcgcagcac ccgcagctcg tccaccgcgg catccgcgcc
    20521 gtcgcggacc agccccgcct ccttcgcgga ggcgggcggc gtctcgacga gcgttgccgc
    20581 gaggcgcgcc gacagggcgt cggggacgcc cgacgggaag gcgcggcccc ccggacgcag
    20641 gagagccgat tccgctccct ccaggatcgg gccgacggcc gccgcctgct cgagccccgt
    20701 gccgagcgac gccaggtcgc gcggcgtcgc gctcccgacg gcaacgcgcg agaggagccg
    20761 cggcaggtcc gggaggcgcg acagcgcggt cgcgaccgcc tccagacgcg caggcccggt
    20821 cacgagctcc tccacggcgt cgagacgctc gtcgatctcc tcgaccgaac cgagcggctg
    20881 ctccaggatc cgtcgaaggg ctcgggcgcc gaacgccgtg cccgtccggt cgacgacgga
    20941 gaggagcgtg ccggtccggc tgccgtcccg aagcgagcga aagagctcga ggtggccctg
    21001 cgtgacggcg tcgacgacga gcccccccgt cgccgactcg gtccggagcc ccgtgacgtg
    21061 ggtgcagtcg gaccgctgag tggagcgcgc ataggcgagg gccgccgcgg ccgcctcgac
    21121 gagaggcccg gaggcgggga gaccgaacgc cgacagcgtg gacgtccgga agtgcccttc
    21181 caggagctcc gcggcgctcc gcccccgcgg cgactcggat gtcaggaccg agaggacggg
    21241 agcctccggc cccagcgcct cgagcaggcg cttcacgtcg tccctctccg aggggaacgc
    21301 gaccagctcc cgggccggtc gccgcgcgag cagctcggcg ggcggggtcc cgtcgaggcg
    21361 gacggccgtg aactctcccg tcgagaggtc gaggaaggcg agaccggcct cgtctccgcc
    21421 gagggcgatc gcggcgaggt cgttcgcctg acgcgcgtcg aggcgctcgg gatcgagaag
    21481 cgttcccggc gtgatgacgc ggacgatcct ccgggcgacg agcgccttcc ccttctgagg
    21541 ggcctcgacc tgctcggcga tcgcgacctt cattccggcc gcgatgaggc gcgcggcgta
    21601 cgactcgaga gcgtggtgcg ggacgccgca catcggagcc tcgacgtcgg tgtcgcggtg
    21661 tcgcgcggtc aggacgaggc cgagcagcgg cgcggccgcc tcagcgtcct cgaagaacat
    21721 ctcgtagaag tctccgagcc ggtagaggag gagggcgtcg ggcacctcct tcttcagcgc
    21781 ccagtactgc tcgagcatcg gcgtcgtgcg catcggaaag gggggcgatg ctagcaggat
    21841 ggggcgcgcc ggacgcggag ccctcctgcc ccgcgcctgc tacgatctcc gccacgccga
    21901 tgaggctcct cgccatcgac acggcgaccc cgcgcccctc gctcgccctc gtcgtcgacg
    21961 gatcggagcc gtggacggtc gagctgccgc agaagggggc cgaggcgctc gcacccgcga
    22021 tctcttccct cctcgtctcc gccggtgcgt cgccccggga cctcgaccgg atcgccgtcg
    22081 tctccggtcc cggctccttc accggactgc gttcctccct cgcgttcgcc cgcggcctcg
    22141 cgcgcgcggc cggagccctg ctcgtcccac ttcccacctt cgaggtcgtc tcggccgccg
    22201 tcccggagcc cccggacgcg gacctcctgc tcgacgccct ccggggcgag gtccacaggc
    22261 gtcggcggcg gaagggccac ctcgcgccgg ccgaggatcg ggtggcccgt tcagaggctc
    22321 tcgaagacgc ggtccgggac ggagtccccg tgctcgacgt cgacgcggtc gcggtcctcc
    22381 tcgcgccggc cgcggccggc ctggcggcga acgcggcacc ggacgggacg gccggcctcc
    22441 tctacgggcg cccgccggcg gccgaggaga ggttcggacc cggaggcgcc ggttgagcgc
    22501 ccttcccgcg gagctcacgg tccgggacgt ccggaacgtc gacctcgacg cgatcgccga
    22561 gctcgagcga gtctcgtttc ccgtcccctg gaaacgggag ttcttcgcgg ccgaggtcgg
    22621 gaccgcccac cggttcaacc gggtcgtccg ggccccgggc ggcctactcg cgggatacgt
    22681 cttctgcgcg ttcgccgggg gagaagtgca cgtcaacaag atcgccgtcg acccctcctg
    22741 ccgccgcagg gggatcgccc gggtcctgat gcaggaggtc ttcgacctct cggagcgaat
    22801 ccgggcggag gagatctacc tggaggttcg accctccaac gtcccggcgc gtaacttcta
    22861 ctcctcactc ggtttccgag aggtcgggcg ccgtcctcag tactacgccg acggcgagga
    22921 cgccctcgtc atgtccctct ttctgacacc ccggccggct tcgccttgac tctcgccctt
    22981 tgaaaaagcg agactcgagc gggctctttc gaaatccact tcgaggaggt gtccatgccg
    23041 actctgaccg aagagttgaa gcgcgagctg gcccagaccc acgacgagtt caagaacctc
    23101 gtccgcaccc atcacgactt cgagaagcgg ctcgccgagc tggcttccaa gacgttcctt
    23161 tcgtccgacg aagagggcga ggagaagcgc ctgaaaaagc agaagctgat cgtgaaggac
    23221 cggatggagg agatcgctcg tgagcaccgg gcccgcgtcg gagcccctca ttagggccgg
    23281 ctcgcgctcg accggaacgg tcccgtcgac aagaccatga acggaacctc gagcgaggtt
    23341 caacgccgcg gcgttcgccg cggcgtttac gtcatcccgg ccctgttcac ggtcgggaac
    23401 gtcttctgcg gctacctgtc tctcatggcc gccgtccgcg gccgctacga cctcgccgcc
    23461 ggtctcgttt tcctcgccgg attcctcgac tccttggacg ggcgcgtcgc ccgaatgacg
    23521 gggtccacct cggccttcgg cgagcagctc gactccctct cggacgtcct ctcgttcggc
    23581 ctcgccccgg cgttcctggt ctttcactgg ggactctccg cccacggccg cctcggcctc
    23641 ttcgcgtcgt tcctcttcct cgtgtgcggc gcctgccggc tcgcccggtt caacgtccag
    23701 atccacgtcg tcgacaagcg ctggttcgtc gggctgccca ttccggccgc cgcgggcgcg
    23761 ctctgcggcc tgatctgggt cattcccgag ccgctcgccg acccgcgcat cgcgacggcc
    23821 ttcctcggcg tgacgctcct tctcggtttc ctgatggtct ccacgttccg ctaccgctcc
    23881 ttcaaggacg tcgacctccg ctcccgccgc tcgatgcggc tcgtgccgct cctcggcctc
    23941 gtcatcgcgg cggtcgccgc ctggcggccc gacgtctgcc tcgcctcgct ggcggtcgtc
    24001 tacgcgctct ccgcgccggt ggcgcgcctc gcttcggcgc tcttcccccg tcggactccg
    24061 ctcgaggacg gcccggcgtg atcggtctct cggtcgccct cgtcgacccg ggctccctcg
    24121 cgggacggga cgtgaaggcc gtccttcgcg agaggagctt tccctcggtc cgcctgcacc
    24181 tcttccacac cggtgactcg acctccggcc tcctgacgga ggacgacgga gaggccgcgc
    24241 tcgcctcccc tctcgagccc gacggcctcg agggatgcca gatcgccttc ctctgcggcc
    24301 cgccggacgc gaccgcgaga ttcctctccc gtcgatcgaa ggacggctgc ctcgcgatcg
    24361 acgtctcggg tcagagaggc gccggcacgc gcgtcgacgc cctccgggga gtcctgccga
    24421 gcccgctccc ggagagcgac ctgctcgtcc tgcgcgatcc gaccgcgacg gtcctggccg
    24481 acgcgatcgt ggcggccgaa tccaccggcg ccctcgccgg cgtcaccgcg gcgatcgacc
    24541 gccccgcgag cgatctcggc aaggacgcgc tcgacgagct gtttcagcag gcgatcgcga
    24601 tcgcctcctt ccggagcatt ccgaaggacc ggctcggcgc gcagtgcgcg ttcaacgtct
    24661 actctccggc cgacacggac gctttcgacg gccgcgtcgc cgaggacgtc cgggagctcc
    24721 tggggcgcga cctccccgtg accacctctc cgcgcggtcg ggcgtcttcc acgggaccac
    24781 gattcgcctg gagctccgtt tcgcggggcc ggccccttcg gccggcgcgc tgcgggccgc
    24841 gctcctgtcg gccggctacg ggtttgccga ggccgacgtc gacggccccg ccgggaccgt
    24901 cgacgccgcg ggccgggacg agacgcttct gctcggggcc tggtccgccg gctcgacggg
    24961 ccggctcctc ctcgcgtccg acgccctccg gcgccccggc gccatcctgg ccgttcgggt
    25021 cgccgaggcc gcggtcgccg accgcggcct cctccccgac gcctgacggc ggcgaccgcc
    25081 gtcttcagcg cttcttcagt cgcggaagag ggggctgacg cttcgccggc gtcctcggcg
    25141 cggccttcgg agacggcggc gccggaatcg cgacctccgc ctcggcgccg tcgatcgtcc
    25201 ggccgtcggg cagggtcagc tgataggtga cgaacatgcg gggtgacttc cccgcgatcc
    25261 ggatcggacc gagctcgttc gactcctcgg gcgcgaagaa gttctcgaag agccggcacg
    25321 ccgtcgcccg gccgaacggc gccgactgcg tcagatcccg ggcggacgcg aagatctcgt
    25381 agcggtcgaa atcgcccgcg ccgagtcccg tgaagacgcc cccttccagc cggatcgtga
    25441 tctggttgcc gaagcgcgag agctcgctcg gagtcggtcc cggattcgag acccggagcc
    25501 agacctcgcc gccacgtgtc ttttcctcga tcgcctcgac ccggaggctc ggctcccacg
    25561 gcttgccggt caggagcgcg cgcaccgcgg ggtatccgct cagccgcccg tccctcggca
    25621 cgccgtcgac gaggaagacc ctccccgaga gccgcggcac gctccaccgc gaggtcgccg
    25681 acacgagccg gacgaggtcg gcgaacggga ggacgtcgaa ccgggcccgc ccaccgtcct
    25741 cggagaggag gttcgtcgcc ttctcgtacc cgagtcgcgg cttgaagctg tagatgttcc
    25801 cgggatcgct cgagaggacc gacgcgaagt cgaagtcgag gttccggtcc tccgacagct
    25861 tgtccatctc gccgtccgga agccggcggg cgcgcgctcc ggagccggcc actccatagc
    25921 tccccgggat cgccaggagg cggaacggga tcgggaacgc cttcgcctcc tcttccgtca
    25981 tctccgagac gaaggcgtcg gccgtctcgg ggcggcggcc gtaggtgagg acgagagcct
    26041 cgtcgacgga acccgcgagc ggcttccacg tcccctcggg cgcgcccgcc cgcagcgtga
    26101 cggacagcgt caggcccggc agccgcgact tcagcgcgcc gagagccgcg gcgagcgccg
    26161 cggcgtcgcc cgcggtcggg acgaggtgaa cgtggactcc gatcacctcc gcccacccct
    26221 tcgcctcggc gacgagccgg gtcgtaccgc tcgcccacgc ctcgccgagg gcctccggag
    26281 cgttgccgcg aagtccgccc tccacgcccg gctctcccat caggacgagg aggaccggcc
    26341 gcggcacgcg cgccgggggc ggcgggaacg accggacctg ccccccggcg gacagggacg
    26401 cagccgccac gtagagccgg cggaccccca tctcgtcgag ctcgggagcg atctcctcgg
    26461 gaacgacgac gggagcctcc gcgaggaaga gcgcgtcgac ccgcgcggcg gcggacgacg
    26521 gacctctccc gccggaggga gcgtccttgc ggcagccggc gctcgcgaac gctccgagca
    26581 ggaggacggc gatggcggcc cgatgcgcat gcagcttgga gcgggacatg gccaaaaaat
    26641 gatatcagcg accggcggcg ggaaggacga agccgatccg aaggcctccg cggaccgacc
    26701 tctccgcgaa gagccggccc ccgtgctcct cgacggctcg gcgcgccgcc gcgagcccca
    26761 tccccgaccc tctcgacttc gtggagaagg tcgggtcgaa gagacgggcg aggtccgcct
    26821 cggcgacgcc tggcccgtcg tcttcgcagg cgatctcgag gccggcccca ccgcgcggcc
    26881 cggaggcggg ccgggccccc acgacgacga cccctccgcg cggctcgagc gcctcgacgg
    26941 cgttcaggac gaagttggag acggcgtccc gaagcagggc cctgtcggcg aagacgtccg
    27001 gcagctcccc gtcgacctcc cagccgacct tcacggccga atgggctccg aagtcggcgg
    27061 cgacttcccg aagcagcccg cggacgctct cgccggagag acgggctcgc tccggcgcgg
    27121 cagggctcgc gaaccgggag aggcgcccgg tcctctccgt caggagctcg acctgacgga
    27181 ggatcgtctc ggccgcctcc gcgacccgcg ccccgtcggg aggagtcccc ctccccgcgc
    27241 cccggagaag gcgctcggcg gtcagccgga tcggagtgag cgggttcttg atgtcgtggg
    27301 cgacggcgcg ggccgcctcc acccacgcct cgagccgctc ggcgcgcgtg aagtcggtca
    27361 ggtcctcgag aaggaggacc gcgcgagggc cggaggagtc cgcggtcagg tcggcgacga
    27421 cgacccgcag gaccgtctct cgtccctccc catggagggt gacgcgggcc tcgaagggcc
    27481 tcgggctccg gagggccgcg agcaccgggg cgtagcgcgg ctccgggagc ctctcgggga
    27541 gagtggtgcc gcccggaagg agcgcgtcgg cggccgggtt cgcgtagagg atcctcccgc
    27601 tctcctcccg gaagaggagc gcggcgggcg tgaggctggc gaggacgctc accgccgcct
    27661 cgcgctcccg ctcgaggagc tcggtccggt cccggacgcg ctcggacatc gtcccgaacg
    27721 cctcgacgag ccgcgcgagg tcctccatct ccggaggccg gatcggaccc ggagcctccc
    27781 cggctccgat cctctccgag gccgccacga gctcctccac gggcctcgag agcgcgagcg
    27841 caccgcggcc gccgagcgcg agggcgccga gcgcgaccgc ggccgcgaga agaaagagcg
    27901 cgtcgacggc cgctcgtccg gccgggtcct ctccgaggac cacggccagc gtcgtccggt
    27961 cctcccgcga gagcgtcacc gcggcctgag cgacccgtcg cccgcccggc cggagcggcg
    28021 cgcgcgtgcg ggcggcggag ctggccccgt ccgcgagcgt ggcggcgagc gcggagggga
    28081 gatgctcggg cgcgagcccg gcggcgaccg ggacggcacg cgacgccgcc gccagccgtc
    28141 cttcgcgata gaggaggaga tcgacgccga cgaccgacgc cgcctggttc aagtcggttg
    28201 gcgacgggct gcccgccacc cggtcctcca ggatccgccg cccctcccga aggagctcca
    28261 gtgcgtgctg ctcggtcgcc cgggccgtcg accggtcgag ggccgtgcgg acgagcgccg
    28321 cgccgagagc gagcggaacg gcaccggaga cgaggaacgc gagggcgagg cgcccccgga
    28381 acgtgccgaa gagccgggct ccccgccgca cgcccgaccg ggcgaggcgg gcccgggcga
    28441 gggccgcgag cttccgccgc aggaggagga ggagggcgat cggcaacgcg gtctcggcgg
    28501 cggcgacggc ggagacgagc gtgaagaccg gagtcggaac ctccacgccc caggcgacga
    28561 agccgccccc cgtgccgcgg acggacattc gccaccggcg tccctgcgac tcttcggtca
    28621 gccagcccgc gccgccccgg cgcacctcgg ccagcaacgg cccggcgagc tccgaccgct
    28681 cgagccggcc ccggccgagc ggccggcccg cgccgtcgaa gtcgatcctc tcgacgggcc
    28741 gatccgcgtg gaccggattc ccgagctccc cgacgaggag cgggtcttcc tcgtccgcgt
    28801 cgggccacgg gattctcgag aggacgccca gggtgtccgg gacggggagc gtgaggccga
    28861 tcgccgttcc gcggccttcg tcccccgggc gcatcgtgcc gaacgaggaa agcacgcgtc
    28921 cctgctcgtc gcgtacggtc aggaggtcgc cgcgctccgg gaattccgcg ttccggcctc
    28981 gcagccagag ggcccgcgcc aggtcgctca tcacggtccg gcttccggcg ggagtccagg
    29041 ggaggaggtc cgggctcgcg atcctctcct cccaggccgc cggcccctct tcctcgagtc
    29101 gctctctctc caacatcgat cggctcgcag ccgcctccag ccgggattcc gcgcgcgaga
    29161 gccgtccgag agccgcgccg gctcccgtca cgagcgcccc cgccccgacg aggagcgcca
    29221 ccgcgacggc cttcgagagc aggtccgctc cagcgaaacg ggccgcgggc cccgacaggc
    29281 cgacgacgag caggaccgca cccgcgagaa gggggaccgt agaggcgacc gacggggacg
    29341 cgaacgcgca ccagacgacg aggccgagcc cgatgacgcc caccgcggcc cgccctcgcc
    29401 ccgggcggag cgcctggccc gcgagaaggg ccccgaggcc gacgaggctg gtggcgacgg
    29461 cgacgaaccc ggcggaggtg aggggtgcct ccagcggtcc ggtgaagagg ccgaggccga
    29521 ggaggagcgt cgggacggcg aggccgccgg cgaggccgag gacggccggc gcgctggccg
    29581 cgagaacggc ggccacgagg gcggcgagcc gcccggccgc gcgctgcggc cgccgtggcg
    29641 cgtcgagcgc cgatcggagg aggacgagcg acacgaagcc cgtgaaggcc gcgtcgaagg
    29701 gcgtggcgag aagaccggtc gggtccgcga ggatccgggc gaagggaccg ccgtcggcat
    29761 ggggtacggc gagaaggagg acgagccggg ccgcgacggc ccccgtggcc ggacggcgcg
    29821 ccaggagccc gagcccgacc gccgcgaccc cgcagaggag ggcgccgacg cgcgaggaag
    29881 aggttcggac gtcctcctcc tcgagctccc tcgggcccgg ctcgacgctc accgcgaaga
    29941 gacggtccgg cgatcgggcc gcacggaccc ggacccggcg ctgggtggcg ccgtcgggtc
    30001 cgcgattcgc gtccaggagg tccggacgga cgattcccgt gggaaagcgg cgggtcacga
    30061 tcaggtagcc ctgcgtctcg gggctccgcc cgagccggga ctcgtgcgag agcgtccctt
    30121 ccgtcacccg gaacgtcgcc gcgaacgggc cggcccgtgg cgccgccccg tccggcagat
    30181 cccccggctc acccgcccac gcgacggcct cgccgcttcg atccagaagg acgattcccc
    30241 agccgggccc ttccgggagc gccgcgccga ggagccgata gacccccccc ggtcggatcc
    30301 cggccgtccc gccctcgacg atctcggtga gcgccgcgtc cgacgccagc ttcccggcga
    30361 cgtccgcgag ccggcgggcg tcgctctcca gcgcccgtcg cgctcgctcc acgagccggt
    30421 cttcccgcct ctcctcggca cccgccggga gaggagcgag gcgcgcggac agcgccgagg
    30481 cgagcccccc tgccgcgagg aggccgagga ggagggcggc cgcgcgacgg agcgtcaacg
    30541 cctcatcggg atcgcgacgc cctgcgcgtc ggcgcaggcg accgcctccg gatacccggc
    30601 gtcgacgtgc cggacgacgc ccatccccgg gtcggagacg aggacgcgcg agagacggcg
    30661 ggccgccgcg tcggtcccgt cggccacgac gaccatcccg gcgtgcatcg aatagccgat
    30721 gccgacgccg ccgccgtggt ggatcgagac ccacgtcgcc ccggccgcgg tgttcacgag
    30781 ggcgttgagc agcggccagt cggcgatcgc gtcgctgccg tccttcatcg actccgtctc
    30841 ccggttcggc gaggcgaccg agccgcagtc gaggtggtcg cgaccgatga cgatcggcgc
    30901 cttcaccttc cccgacttca cgagctcgtt gaaggccagc cccgccttct cgcgctcccc
    30961 gtagccgagc cagcagatcc tcgcggggag accctggtag gcgacccgct tcgccgccat
    31021 gtcgagccag cggtggaggg ccgcgttctc ggggaacagc tcgacgagcg cgcgatccgt
    31081 ggcggcgatg tcggccgggt cgcccgagag cgcggcccag cggaacggcc ccttcccgac
    31141 gcagaaaagg ggccggatgt acttcgggac gaagccctcg atctcaaagg cgttctcgat
    31201 ccccgccttc acggcctgag cgcgaatgtt gttgccgtag tcgaacgtca cggcgccccg
    31261 gcgctggagc tcgagcatga gccgcacgtg ctcgcccatc gaggcgaccg agcggcgccg
    31321 gtactcctcg gggtcgctcg atcggagcgc gagcgccgcc tcgtacggga gtccgtgcgg
    31381 gacgtagccg ttcagctcgt cgtgagcgga ggtctgatcg gtcaggaggt ccggcacgat
    31441 cccgcgctcg acgaggcgcg cgaggaggtc gacggcgttc gcgaggacac cgaccgagat
    31501 cccctccttc gcgtcgcgcg cggcgagggc gcggtcgatc gcggcgtcga ggtcgtcgat
    31561 ccgctcgtcg cagtagcggg tctcgaggcg gcggtcgatc cggtgcgcgt cgacctcggc
    31621 gacgagcgcc gtgccgccgc ccatcgtgac ggcgaggggc tgggcgccgc ccatgccgcc
    31681 gaggcccgcg gtgacggcga gcttcccggc gagcgagccg tcgggcgagc cgaggtcgcg
    31741 gcggcccgcc tcggcgaacg tctcgtacgt cccctggaga atcccctgcg tcccgatgta
    31801 gatccaggag ccggccgtca tctggccgta catcgtcagc cccagcgcct cgaggcgccg
    31861 gaactcgtcc caggttcccc acttcgggac gaggttggag ttcgcgatga ggacgcgcgg
    31921 cgcgtcggcg tgcgtgcgga agacggcgac gggcttcccc gactggacga ggagcgtctc
    31981 ctcgtcgccg agccgctcga gctcgcggag gatcgcctcg aggcactccg ggttgcgcgc
    32041 ggccttgccc gagccgccgt agacgacgag gtcctcgcgg cgctcggcca cgtcggggtc
    32101 gaggttgttc aggagcatcc ggtagggcgc ttccgtgagc caggagcggc accggagcga
    32161 cgtcccgcgc ggcagggcgg gcggagcgta acgggcgggg gcggagacgg tgctcatgac
    32221 gcggatgtta gtccggaagg cgctccgacc gccgccaccg aggtcgccgc cgagcgacga
    32281 agcggcgcgt caacgcgccg cgctacttga cattcattac gcatcgcgtc acattgcccg
    32341 ggcgatgacg agccaccaca gggagatcgt tcgataccgg aaccggaagc tctacgagaa
    32401 gagcgagcgc cgcttcgtga cgctcgacga catcgcgcgc gccgtcgccg ggggcgaccg
    32461 cattcgcgtc gtctccgccg acacgggcga ggacatcacc gcccggatcc tctcgcgcgc
    32521 gctcgcgtcc gaccgcgtcg ccttccccgc ctcgaccgac gccctcgccc tcctcctccg
    32581 cgccgggagc gacgcggccg agacggtggc cggcgtcgcc gagaaggtcg gggccgcgcg
    32641 gatggcgggc gccgtccgga aggcggcgca gccggagcgg atcgccgaga cgctcgcgcc
    32701 cgtcacgcgc agggtcgaga ccgcacgcca cgacgtcgag cggatcgtcg ccggtctcgt
    32761 cggccgggga cggctgacgt gggaggaagg cgcccggctc cgcgaggacg tcggcacggt
    32821 ctttcgggag agtctctccg acgtcgtcgg acgggtccgg gacgtcgtcc ggaaggtctc
    32881 ccccgcctcc tcccccgcga tccacgcgga gatccaggag ctcgcccgcc ggctcgacca
    32941 cctcgaggcc ctcgccaccg cctcgttccc gaaggggagc tccgccgcgc cccgaaacga
    33001 tcacgccgcg cgccccgcgc cggcacgaag gaaggagacc agaaagccat gacgaggaag
    33061 acgacccgcc gagccgctcc cgccgccaag tcgctcccgt tcgacgtccc gccgcaggtc
    33121 gatcgggccc tccggagcgc ggtcgagaag gtcgacgccg tgagggacga gacccgcacc
    33181 gtcgcctcga aggtggagaa gacggtccgg gccgccgcca cgagcgcgaa gaagaccggc
    33241 gagactctcg cccgtgaccc gaagcgcttc gtcgaaggcg tcgtccgcga cgcgaagtcc
    33301 aacctgaaga ccgtcggggc cgacgtctcg cgcgaggccg gacgcttcgc cgacgaggtc
    33361 gcccgccgcg tctcgggcgt cgtcgagccg gctctcgaga aggccctcca tcgcctgaac
    33421 gtcccgacgc agaaggacct gaaggccctc gtcgcgaagg tggacgcgct cggccggaag
    33481 atggagcagc tgcacgcccc ggcccgcaag ccgcggaccc gcgcgcgcgc ctgaccccgg
    33541 gctctccgcc ccgatccgga tagactggcg ccggcgcccc cggcgcccgt cgtcttttca
    33601 ggaggttccg tggcggcacc ccggctacgt ctcgtctcct cgcgcggtcc ctcgtctccg
    33661 cgaccgctgc ccagccgtcg cgggctcgtc tgcgccggtg gcgggatcac cggagcgatc
    33721 tacgagatcg gcgtcctcgc cgccctcgaa gaccgcctcg acggcttctc gctcacggag
    33781 tgcgacgtct tcgtcggcgt ctccgcgggc gcctacgtgg gggcgctcgt cgcgaacggg
    33841 acctcgccct ccgtcctcta ccgcaacgcg acccacccgc gcggagaggg gctcgaccgg
    33901 ctctcattct tccggccgaa cctcggcgag atcgtctcgc gtgccgtgtc ggctccccgg
    33961 atcgtcttcg aggcgctccg cgacctccgc gagagcccgg gggacctcac gctgaccgat
    34021 ctcgtcgcct cgctcgcggg tctcctcccc tccggcttct tcctcctcga cggcctcgag
    34081 gcatggcttt cggagtggct ctcccagccg ggccgaacga acgacttccg gaccctgcgc
    34141 cgagaccttc ggctcgtggc ggtggccctc gactcgggcg agacgcgcgt cttcggcgcg
    34201 tcggccggcg agaccgtccc gatctcgcgc gccgtggccg cctcctgcgc gctgcccggc
    34261 ttcttccgtc ccgtccggat cgacggggtc gactacatcg acggcggcgt gaggaagacc
    34321 gcgcacatct ccctcgcgct gaaggatcga tgcggactcg tcctctgcgt gaacccgatc
    34381 gtcccgctcc gcttccccct ctcgccggcc ctctctcccc gccatccggg gcagggagcc
    34441 ctcgccgggc gcgggctcct ctccgtcctc gaccaggtct tccgcctgtc gctccactcg
    34501 cggctccggt acggcctcga ccgctaccgc agggagtacc ccgacgccga catcgtcctc
    34561 ttcgagccga aggccgagga gctcccgcgc ctcatgcgga acatgatgag gacgtcgggc
    34621 cgggtgcgaa tcgccgagta cgcctaccgc acgacccagc agaagctcga cgcggagtac
    34681 gggcgtctct cgcgcgtctt cgagcgccac ggtctccggc tccgcccggc cacgtccgtc
    34741 cgtcgggcgc tgcgccgccg gacggcggcg cagtcctccg cgtcctgagc ccggcctact
    34801 cgccggtgaa gacgttcccg cgccgcgcca ggcgcgagtc gatcatccgc tggatcgtcg
    34861 accggacgag gtccgtcacc tcgttgacga tcatcggccg ctcggcgtag gccgggtcga
    34921 ggcccgcgtc gatcggcgcg ccgacgtcga tccaccactt cgtcggcgcc ggcacgaggc
    34981 cgagcggccc gaggagcggg aacgtcggcg tcaccgggaa gtaggggaag ccgagcggac
    35041 ggccgacgaa cgcgaggttc aggagcatcg ggtagatctc ctcggctccg acgaccgaga
    35101 ccggaatgat cggggagcgg gtcttcaggg cgaggcggac gaagccgccg cgcccgaagc
    35161 gggcgacccg atagcgatcg cggaagtact tcccgacccc cttcaccccc tcggggaaga
    35221 cggcgacgag ctcgtccctc tcgaggagcc tctcgccgtt ctccgggcag gcacggacct
    35281 cgcccatctg gcggagccag ggctggagga acgggagggt cgtgaacatg tcgagggcca
    35341 gcatccgggc ctgtcgccgc gcgggatgct cgtgcgcgat cccgtgcttg agcatcgcgc
    35401 cgtcccaagg caggaccccc gagtggttga tgacgaggag cgcgcgcccc tcgttcggga
    35461 cgttctcgac gccggtcagc gtcacgcgcc accagacccg gtagaggaag tcgaagagcg
    35521 ggtgccagac tctcgccgcc gcctcgtccc agccgaagtc gtcgagctcc agcgactggt
    35581 tgcggtgaag gaagaggttc cagaggtcga tccaggtcct cggcgaggcg agctccggat
    35641 gcgcgacgag gagcttcccc gcctcgcccg cgagctcgcg gaggagcttc gcgtcgggcg
    35701 ccgcggggac gaggctgggc gcgaacggct tcgccggctc cgcatgcgcg gccgcgggcc
    35761 tcgtgctcat gccgtcctcc gggtccccgc ggggcgggcc ggacgctccg gctccccgcc
    35821 ggacgccgcc ggcgcgggcc ggcggagacg ctgctcgagg cgccacgtcg cggcgcgcac
    35881 gacctcctcg accgcgtcga agtcgatcgc ccggccccgg ccccgcctcg tccgcgcggc
    35941 ctcgacgacg gtctcgagcg tcgtcctctt cggcgagaac ccgaggacgc ggcgcgcctt
    36001 tccgttgtcg gcgacgcagg ggaaccggac gtagtgcgca tgagcgccgg gcgccgtggc
    36061 cagcccggcc agccacgcgg cctcggccgc cgtgtaggcg agggcgtgcg gcagcggaac
    36121 ggagaccgta ccgaagagga ggagcgtcga ggagagcggg aggacgccgt ccggcgcgac
    36181 gttgaagacg ccgtgggcgc cgggcgtgtc gaccgtccgg aggagggcct cgctcgcgtc
    36241 gtccgggtgg agccactgaa ggagcgggtc gtacccgagc atcgtcaggg cgaccggtcc
    36301 ctcgagatag cggatccggt agtcccgccg ctccgcggcg aggatcggcg cgaagcgcag
    36361 gacggcgact ttcgcgtcgg ggtgtcgccg ggcgaagtcc cggcaatgcc tctccgcctc
    36421 gaccgagtcc cgcacgaagc ggtcgctcac gtcggcgcgc atcgggtgct cctcggagag
    36481 gaacgccggg ttgtccccgc gggcgccgta gaccatcgtc gtcgagtagt ggacgaggcg
    36541 ccgcaccccc gcctcgcctg ccgcgccgaa gacgttcagc gtcccgatcg acttcagctc
    36601 gtgggagtag acctcgtccc ggagcgggct ccggagtccc gccaggtgga cgaggacgtc
    36661 ggggcgctcc tccttcagga tcttgaagac cgtcccgtcg gagtgcggca ggttgaggtc
    36721 gaggaagcga tgccggacct cgggcggggc gttcgccgcc ggcgcgaggt cgaggacgac
    36781 gacgtcccac gtcccgcggg ccgcgagcgc ccggacgaac cgcgcgccga gatacccgct
    36841 ccctccggtg acggcgacct tcggtcgcgc gcccgccgcc ggagggaagc cgacgacctc
    36901 gccgcccggg cggcgacggg gccggggccc gcccgctctc tccggtccgg tcacggcggg
    36961 gcccgccttc agccggcctt ccggcggacg ggcttccgcc gcaccggagc cttcttcgac
    37021 gactcgagct ccgcctcgag cgcagcgatc cgtgcctcca tcctcgcgag gtcccggcgc
    37081 gtggcgacgt tcatccgctt caacgcgacc gcgacctggc cgtcgacgac ctccttgacc
    37141 gcgatccccc tcttgagcat ctccacgaac gcttcgttgg agagcaggcg attcgtcacc
    37201 tcggtgacga cctcctcgcc gcgccccagg agctgtcgga tcagagtctc ggccatcgtt
    37261 cgtccttcct tccgcacgcg aatcggacgt ccgtcagcga ccggcctggg cgaggcgtcc
    37321 gtgcgagcag ccattatcca cgcttcgccg acgggcaatc gagcggagaa tgcggccgat
    37381 ggctccggcc gaaccgctcg acggcctgac cggcgcgcgc ctccttttcg tctccggaaa
    37441 gggcggcgtc ggaaagacca cggtcgcttc cgcgctcgct gcccggcagg ccgcctccgg
    37501 gtggcgcgtc ctgctcctct cgaccgacgg ccggggcgac gccgccgcgc tcttcgggcg
    37561 ggccgaccgc ggctacgagg agaccgagct cgcgccgcgt ctccggacgg tcaccgccga
    37621 gttcgacgcc ctcctcggcg acttcgtccg gacgatcgct cccgtcggct tcgtcgcctc
    37681 gcgcatcctc gcctcgccca cgttccgcta cttcacgcgc gcgacgcccg gccttccgga
    37741 ccttctcctc ctcggcaagc tccgcgagct gctcgcgccg aagaagcccc gcttcgacct
    37801 cgtcgtcgtg gacgctccgg cgacgggcca cgcgctctcg ctcctctcga tgccccgcac
    37861 ggtcctgcgc gtcgtgcccg ccggccccat ccatcgcatc gccggcgacc tcgacacgct
    37921 cttcgcggac ggccgatccg cggcgctcgt cgtcgtcgcc gagccggctg agctggccgc
    37981 ccgggaggcg gaggagctcg tcgcggaggc cggtgcgcgc gccggcctca gggccggcct
    38041 cctcgtcgtg aaccgcgtcg gccggagcgg ccgcgccgga acgctgccgc gcggggagct
    38101 ccccaccata cgggtccccg agctcgacgt accccgcggc gccacggaga gcccgttcgc
    38161 cctcgacgag gcgttcttcg gatcgttccg gcgcgcgttc gagggctcgc cgcctcctcg
    38221 gcgccggggc ccgtctcctc cgcctcctgg tcccggcgac ctcgacctcg acgaggcttt
    38281 gcgcgaggag ccgctcgtcg tcctggcggg ccctggcggg gtcgggaaga cgacgctcgc
    38341 ggccgcggtc gggatcgcct ccgcgcgggc ggggcagcgg acgctcgtcc tgacggtcga
    38401 ccccgcccgt cgcctcgcgc aggcgctcgg cctcgagggc cgcctcgacc ggcccgtccg
    38461 ggtcgacctc cctggcgcgg ccgctctctc cgtcctccag atcgacccgc gcgcgacgtt
    38521 cgaccgcctc ctcgcgcgag tcgccccgcc ggccaccctc gctcgcatcc ggtcgaaccg
    38581 tctctacgac ggactcgtcg acgctctccc gggggtcgtc gagtacatgg gcgtcgaggc
    38641 gctcgccgag cacgccgggg accccggcat cgaccggatc gtcctcgaca ccgcgcccgc
    38701 cgcccgcggc gtcgacttcc tgaagtctcc cgagcggatg gtcgagctcc tcgagcacga
    38761 cgccctccgc tggttcctcc ggagcgactc gctcctctcg aaggctctct ccggcgcctc
    38821 ccgcggcgcc gccgtcgtcc tccacctggc cgatcgtcac ctgggattca gcttcctcgc
    38881 cgacctggcg gagttcttcc gcgcgttcga cggcctctac gacggcttcc gggagcgaag
    38941 cgtgaggatc cgcgccctcc tcgacgacgc ccgctacctc gtcgtctcct cggccgaccg
    39001 ctcggcgctc cggacggcgg ccgagctcgc ggagctgctg ccggcggggc ccgggaaggc
    39061 ggggctcctc ctcaaccgcg tctccccgcg gttcggcctc cccccgctcc ccccgcccct
    39121 ccggtcgctc ccgaggcgca acttcgtcga ggacgcgggc tccgcggagt cgctcccctt
    39181 ccggctcgcc gaccagctcg tctcggccga ggtggcttcc ccctcctgac tactcgagac
    39241 gggcgaggag gtcgtcgagc cccgcgacga ggacgtcccg cgccgcgtcc atgtcggccg
    39301 cggcgtacct gtcggcgtcc cagggggcga cgcgggcccg gagccgggcg tggagctcct
    39361 cgagcggcgg gctcgagcgg agcggccgcc gaaggtccag tgcctgggcg gcggcgagga
    39421 gctcgatcgc gaggacgttc tcgaggctcg cgacgtttcg cgcgagcttc cgcgccgcga
    39481 tcgggcccat cgaaacgtgg tcctccttgc cggccgacgt cggaatcgag tcgacggagg
    39541 ccgggaagga gttcgccttg cactccgaga cgagagccgc ggccgtcacg tggatcatca
    39601 tgaagcccga gtggagcccc ccgtccttca cgaggaaggc gggcaggtcg cccgagagcg
    39661 tcgggttgac gaggcgctcc gtccgccgct ccgagaccga cgccaggtcg gtggccgcgg
    39721 ccgtcagaag gtcgaagacg agcgcgaccg gcgcggcgtg gaagttcccg cccgagatca
    39781 gctccccctc gccgtccggt ccggtggcga agaccatcgg gttgtcggtc gcggagttca
    39841 tctcggtcgt cacgacgcgg agggcgtggg cgcaggcgtc gcgcaccgtg ccgtggacct
    39901 gcggcgcgca gcggagggcg taggcgtcct ggaccgtgcc gcagtgccgg tggctctccc
    39961 ggaggggcga gcccgcgagg agggcgcgca ggttccgggc ggaggcggcc tgcccggggt
    40021 ggggccgcgc cgcgtggatc cgggggtcga aggcggcgtc ggtccccttc agggcgtccg
    40081 tcgtcagcgc ggtgacgagg tcggccgcct tcacgagcga gaggagacgc gcgagggcga
    40141 ggccgccgac cgaggtcgtc agctgcgtcc cgttgatcag ggcgaggcct tccttcgcct
    40201 cgaagaccgc cggcacgagg ccggccgcgg cgagcgcctc ggccgccggg acgacgcttc
    40261 cgcgatgctc gacctcgccc tcgcccatca cggcgcgcgc gaggtgagcc agcggcgcga
    40321 ggtcccccga cgcgccgacg gagccctgcg agggcacgcg gggatggatg ccggcggcga
    40381 gcatcgccag gaggagctcc accgtctctc gcctcacgcc ggagaagccc cgggcgaggc
    40441 agttggcccg caggagcatc agggcccgga cggccggtgt cggcaggagc tcgccgacgc
    40501 ccgcggcgtg gctctcgacg aggttccgct ggagctcgcg gagcttctcc cgcgggatga
    40561 cgacgtcgga gaacttgccg aagcccgtgg tgacgccgta gacgacgcgg tcctcgcgga
    40621 cggcgcggtc gatgacggcg cgggaggcgt cgatgcgccg ggcggcctcc tcggagagga
    40681 cggcggcgcg gccgcgccag gcgaccgaga cgaggtcgtc gagggtgagg gattcgccgg
    40741 tcagggtgac gggttcgctc atcggtcggc gattctcgca ttcgccgggg gcctcgcggc
    40801 ggcgggtaga atcgacaggt gaatacgtct cgcaacgccg tcgtgatcgt cggggggcag
    40861 tggggcgacg aggggaaggg gaaggtcgtc gacctgatct cccccgcctt cgacgtcgtc
    40921 gcgcgctacc agggcggcca caacgccggc cacaccgtca agttcgagga ccgccatttc
    40981 gctcttcggc tcatcccgtc cggcatctgc cggaagggcg tgctgaacgt catcgggaac
    41041 ggcctcgtcg tcgacccgcg ggcgctcgtc gccgagatca cgagcctccg cgccgccggc
    41101 gtcgagatcg gcgagaacct gaagctctcc gacagggcgc acgtgatcct cccgacccac
    41161 gcgctcctcg acggcgcgcg ggagaacgcg ctcggtggcg cgaacatcgg gacgacctcg
    41221 cgcggaatcg ggccggccta cgagtcgaag gcgaaccgga gcggaatccg ggtggccgac
    41281 ctcgtcgacc ccgaccgttt cgtctcgctc gcccggccgc tcctgggcca tcacgcggcc
    41341 gtcctctcgc acttctacgg cgtcgaggcg ccctccgtcg acgagacgat cgagacctac
    41401 ctcgcgtgcg gccgggacct ggcggcgcac gtcgccgaca cgcgggcgct tctgcacgac
    41461 cggctcgccg cgggggcccg gctcctctgc gagggggccc agggcgtgat gctcgacgtc
    41521 gaccacggga cctacccgtt cgtcacgtcc tcctcgtgcg gcccgggcgg ggcctgcacc
    41581 ggcctcggga tccctccctc cgcaatcggc gccgtcgtcg ggatcatgaa ggcctacacg
    41641 acgcgcgtcg gctccggccc cttcccgacc gagctcttcg acgagaccgg ggagcggatc
    41701 cggaacaggg ggaacgagtt cgggacggtg acgggtcggc cccggcgcgt cggctggttc
    41761 gacgcggtcg tggcacggac gagcctgaag gtcggcggcc tcgacgccat cgccctcacg
    41821 aagatggacg tcctcgacga gcacgacgag gtccggatct gcgtcgccta cgagatcgac
    41881 ggccggacca tgaccgaggt cccggcccgc tccgaccact acgaggcggc ccggccggtc
    41941 tatcgcacct atcccggctg gaagcgatcc atcgtcgggc tcgacgacgt ctcgaggctc
    42001 cccgaggcgg cccgggccta cgtcgccgcc ctggaggaga ccgtcggcgc cccgatcacc
    42061 ctcctctcga cgggcccgag gcgggaggag acgattattc gacccgggac cgtcctcgac
    42121 acctggcttt ctcccgcgcg gcgctgagct ccggcgaggg gggcgggagg aggacgtgac
    42181 catcgcgggc gtcctcctcg ccgcgggcgc ctccaggcgc ttcggaggcg agaagctcct
    42241 cgcgcccctg ggcggctcct gggtcgggat ccacgccgcc cgggtgttgc gcccggccgt
    42301 cgaccgcgcc gtcgccgtcg ttcgtcccgg ggacgagcgg ctcgcggacc tcctggcctc
    42361 cgagggattc gagacgctcc ccttcaccgg cgccgcccgt ggcatgggtg cgagcctggc
    42421 cttcggcgtc gcgagctcct cgaaggccga tggttggctc gtcgccctcg ccgacatgcc
    42481 gttcctcgaa gccgcgaccg tcgcccgggt cgccgagagg cttcggctcg ggatcgatcg
    42541 gatcgtggca ccgagcttcg gggggcgccg aggccacccc gtgggctttc gtcgggacct
    42601 ccttccggaa ctcctcgccc tcgatggcga ccgtggcgct cgcactctcc tggccgagca
    42661 cgcgacagaa gtcctgctcc tggagtgcga tgacgaaggg atcctgctcg acgtcgattc
    42721 gacgcgggac cttgacagag accgctcgag cgaatagata ataaattttt ctgatacaca
    42781 aaaattttct tgacacactc aaaccgcgag ctagagtcgg tgtggacgcg tcgagcgctg
    42841 ccccaactcc cgcgacggga ccgggacgac gaagagcctc gcggtcggcc ataaaggccc
    42901 ccgctcctct cccccgcccc tcggtcgagc tcgagcgttc gttacgggtg cgctcctcgg
    42961 ccccggcgcc cgcgccccga agagaaccgg aacggaggat ctgatgtccc agctcgattc
    43021 ccgcatcgcc gcgctcgccg cgcagtacct ttccctcgcc aaggagaccc tcgcggaggc
    43081 gatccggatc ccggccgact acgtcgacaa gccgctcgag aagggcggag acccgctctg
    43141 cggcacctcg aaccacgagc agccgcggct cgagtacctc cggaagaaga tcctcgagat
    43201 cggggcggtg cgccggcccg aggacgtctt cttcgacggc ttcggcaacc tcgtctggca
    43261 ggtcgacgac ccgaacgacg gcattccggc ggcgcagaag aaggtcgtct acatcgacgg
    43321 ccacaccgac accgtcaagc cgctccgcgc ccagtggaag gagcgggtcg tcggggccga
    43381 ctgcttcgac ggtctcgtcg acgcggcgaa gctcgacaag gcgttcctga agaaggagct
    43441 cggatggacg ccgcccgacg ccgagtgggg gcacctcctc ttcggccgcg gctcggccga
    43501 ccagctcggc ggcgtcgtct ccgcgatcat cgccacgaag atcctcgtcg agctgaccgg
    43561 ggaaggggcg ctcgcgggcg ccatcgtccg ctcctacggc accgtctgcg aggaggacaa
    43621 cgacggcgcg ggcccgatgt acctcaaccg cgaggtcttc ccgacggccg gccccgaggt
    43681 gattcccgac gtcgtcatcc tcacggacgg cacgggctgc agcaagcgcg gcgcgctcgg
    43741 catctaccgc ggccagcggg gccggatgca gatcgaggtg accgtcaccg gaaagtcgtg
    43801 ccacggctcg atgccgtggg aggggaagaa cccgctcgag tacggcggcg ccatcgtgaa
    43861 ggaggccgcc gaggcgtacg agaagcgcga cggcttcctc gacgacccgt tcctcggcca
    43921 cggcacccgg acggcctcct gggcgcgtct cgacacgccg agcgactgcg ccgtccccga
    43981 gaagctcgtt ttccgtttcg accgccgcct gacgatcggc gagacgcccg atcaggccgt
    44041 cgccgacgtc gagcgcctct cctccgtcgc cgccgcccgc gaggccggcc tctccgtcga
    44101 ggtgacgatc cccacgtaca ccgacgcgag ctggaagggg tacgccctcc acaacccgca
    44161 ggtctacctc ggctgggtca cgccggagga gcacccgtcg atccgcgcgg cggtggagac
    44221 gtacaagagc gtcgtcagcc cgcacgtcga cgggaagatc ggcgaagggg gcgtcctgac
    44281 gaaggagccg cgcgtggagc gctgggtctt ctcgaccgac ggcgtcggct tccccgtccc
    44341 gaaggaggcg aagatcccgg gcgccgcggg gaaggcctgg gtcgagagcg gtccctacaa
    44401 gtacccggcg atgttcggct tcggcacggg catcgagcag aacacccaca agatcggcga
    44461 gtgcctcgac acgcgcgagc tgatccacgc ctccgcgttc ctcgcccgct tcccgagcgt
    44521 cttcgtcgcg acgaagtagc ccccggggcc cggcgcggcg tgagccgcgc cgggcctttc
    44581 gaagtccctt ccgtccgagg aggccgatcg tggaaaaggc gttccgtccc atccctctcg
    44641 agctcctcgc gcggtgggtc ttcggcgagc tcgactcgcg caacacggtc ctgggcgtcc
    44701 cgaaggcctc gttcgcgcgg cccgacccgc gcttcgcctc ccgcctcttc ggccgggacc
    44761 tcgcggcgcc cctcggcgtg gcggccggcc cgcacagcca gctcgcgcag aacatcgtgg
    44821 ccgcctggct ctcgggagcg cgcttcgtcg agctgaagac ggtccaggtc ctcgacgagc
    44881 tgagggtgag ccgcccctgc atcgactcgg ccgacgagac gttcaactgc gagtggtcgc
    44941 aggagctgaa gctcgagcag agcttcgacg agtacctgaa ggcctgggtc ctcgtccacg
    45001 cgctcgcggc gaagctctcc ctcccctcgc ccgggacgct cttcgcgatg agcgtcggtt
    45061 acgagctcga ggggatccgg agcgaacgcg tccaggagtt cgtcgcctcg atccggaacg
    45121 cgaaggacgc gctccccgcg gccgtcgacg ccgtcgcgag ggcgtggccc ggcgtccgcg
    45181 acgtcgaggt cccctcctcg atctccgacc acgtcacgct ctcgacgatg cacggctgcc
    45241 cgccggccga gatcgagaag atcgcgctct acctgctgca ggagctcggc gtccacacct
    45301 gggtcaagct gaacccgacg ctgctcggcc ccgagcgcct ccgcggcctc ctcaacgaga
    45361 cgctcggcta cggcatcgag gtccccgacg ccgcgttcgg gcacgacccg aggttcgagg
    45421 atgccctccc gatggtgaag cgcctcgccg cggcggcgcg ggccgtgggc cgcgagttcg
    45481 gcgtgaagct ctcgaacacc ctcgaggtcg tcaaccaccg ccccgtcttc cccgccggcg
    45541 agaagatgat gtacctgtcg gggcgggcgc tccacccgct cacgcttacg ctcgcgaagc
    45601 tcgtcgacga cgagctcgac gggagcgtcc cgatcagctt ctgcggcggc gccgacgcct
    45661 ggagcttcgc tgacctcgtc gccgacggcc tgacgccggt gacggtctgt acggacctcc
    45721 tcaagccggg cgggtacgcg cgcctcgccc agtacctcga gaacctcggt gccgcgatgg
    45781 acgcggcggg agcctcggac ctcgacgcgt tcgtcgcgag gacctccggc gggacggggc
    45841 gtcgcgggaa cctcgcgagg cacgccgggc gggtcgccgc agagaggcgc ttcgcgcgcc
    45901 gcgagcgtcc cctcgccttc aagggcgagc gccccctcgg gcatttcgac tgcatcgcgg
    45961 ccccctgcgt ggaggcctgc ccggcccacc agaacatccc cgactacctc tggctcgtcg
    46021 cgcgcggccg cgagacggag gcgatcgagg tgatccgccg gtcgaacgcc ctccccggtg
    46081 tcacgggcag catctgcgac cacccgtgca ccgagcgctg cgtgcggaac ctctacgacg
    46141 ccccgctcgg catccgcgag atcaagcgct tcgccttcga gaacggcgtc tcgaagcccg
    46201 aggtcgcggg agccccgatc ggcgtgaagg tcgccatcgt cggcgccggg ccggcgggcc
    46261 tctccgccgc cctgaacctc gcccgcgccg gcatcgcgtc cgagatcttc gaggcgaagg
    46321 ggcgcgtcgg cggcatggtc ggcggcgccg tcccgaggta ccgcctctcc gacgagaagc
    46381 tcgcggtcga cctcgaccgc cttcgctccc tcggcgtcgc ggtccacctg aacgcggcgg
    46441 tcgggaggga cgtctccctc gacgacctcc gcgcgcggtt cgactacgtc ttcctcggcg
    46501 ccggcgccca gaaggggaaa aagctcggga tctccggcga ggacgccgaa ggggtcctcg
    46561 acgctctcga cttcctggac ggcctccgcg agggggtgaa gcgcgacctc ggcaggcgcg
    46621 tcctcgtcgt cggaggcggg aactccgcga tggacggcgc ccgatcggcg aagcgcctcg
    46681 tcggcaggga cggcgaggtc tcgatcgtct atcgccggac gcgcgccgag atgcccgccg
    46741 accccgagga ggtccgcgac tgcgacaccg aggggatcgg cctgaagacg ctcctcgccc
    46801 cgctccgcgt cgtcgtcgaa aacggccgcg ccgtcggcct cgcctgccag ccgatgaagc
    46861 tcggagagcc cgatgcctcc ggccgcccgc ggccggtccc ggtcgagggg agcgaggtcg
    46921 tcctcccggc cgacacgatc gtcaccgcga tcggccagga gacggcgctc gagctcctcg
    46981 ggggcgccgc cgtcgagacg aggcggaacg ggaccatcgc ggcgaagccc gcaacgggcg
    47041 agacgtccgc ggcgcgcctc tacgccggcg gcgacgtcgt ccgcggcccc gcctcgatca
    47101 tcaaggcggt cgccgacggc cgcgccgcgg cggaggcgat cgcccgcctc cacggcatcg
    47161 agcccgtcgc cgagccacac ctgccgaagg acctctcggc cgcggccgcg ctcgcgaaga
    47221 aggcgcggct cgcggccccc gagccggtcc ccgagcttcc cgtcgccgag cgggtcggct
    47281 tcgacgaggt tctgcggccg ctgagccccg aggcggcgcg ccgcgaggct tcccgctgcc
    47341 tcgactgcga cgaggtctgc agcctctgtg tcaccgtctg cccgaaccgg gcgaacctgg
    47401 cgtacgaggt ctcgcccctt tcgatcgagc tcccctcctt cgtcgtcgag ggagggcgcc
    47461 ccgtcccggc ggggacgacg gcgctccacg tcgcgcaggg gatccagatc gtcaacctgg
    47521 gcgacgcctg caacgagtgc ggcaactgcg tccccttctg cccgacctcc ggcgcgccct
    47581 ggcgcgacaa gccgacgttc tggctcgacg ccaccggctt cgccgaggcg aagggggacg
    47641 cgttccggat gacgcgcgac ggcgacgcgc tcgtcctcga cgcgcggatc gccgaccgcg
    47701 cccatcggct cgagcaccac ggcgccctcg ccgagtaccg ctcggattcg ctccgcgtcg
    47761 tcctcgagcc cgcgggctgg cacgtcctcg acgtccggct ggagccaggc gccgtcgagg
    47821 gcgatcgcct cgacctcgcc gcctgcgccc tcctggtcgc cctcctgccg gccgagagcg
    47881 cgctgcctcc gctcccggcg gggaacgcgg gagggcgcgc gtgaagacgc tcgtgaggaa
    47941 cggccggatc gcgtccgacg ggcgcgtgga gagcgcggac ctcctcctcg cggatggagt
    48001 cgtggccgcc gtcggccacg acctcggccc ggccgaaagg gagatcgacg cctccgggtg
    48061 ctgggttctc cccgggctcg tcgacctcca cgtccacgtc gccgaccgga tcggccgctt
    48121 cgagctggcc gacgactacg agagcggcac gcgcgtcgcc gtgaagaacg ggatcacgac
    48181 gctcgccgcg ttcgtcacgc aggggccggc cgagtcgctc ggagaggccc tccgccgggc
    48241 ccgcgcccgg gcggccggcc gctgccacgc cgacgtcacc tggcacctga cgccgacgag
    48301 gttcgaagcg gccgactggg cggagatgga gtcgctcgtc cgggcgggct accgcacctt
    48361 caagctctac acgacctacc ggggcgccgg gctcttctcc gacgccgtcc gtctcgacga
    48421 gctcttcgcg cgcctcggcc cgctgggtgt ccgtttcctc ctccactgcg aggacgacgc
    48481 gctcctcgag gcggtcgaca cgtcgtcgat cgacctctcg agggcctcct cccacgcgaa
    48541 gctccggccg gaagccgccg aggtctccgc cgtcgaggcc ctcctcgacc tcgcctgcag
    48601 gcggggcgtc ccccttcacg tcgtccacgt ctcgacggtc ggggcggccg agcggattcg
    48661 cgacgcccgt gcgcggcagg acgtcacctg cgagaccggc ccgcagtacc tctggctcga
    48721 cgagacgtcg ctcgaccgcc ccgacggcca ccgctggctc tgctcgccgc cgctccgcgg
    48781 agaccgcgag cgcttccgcg cgctcgccct cgaaggggcc ttcgacctct tcgcgacgga
    48841 tcactgcccg ttccggaagg ccgacaagga cgactgggac ggccgcgacg tacggacggt
    48901 cgcgaacggc cttcccggcc tcggcggact cctccgccac gcctggaggc tctgggccga
    48961 cgagcccgac cgggccgcgg cggaggtctc ccttcgtctc tcccggaatc ccgcacttcg
    49021 cctcggcctc gacggccgaa agggggcgct acgtcccggc ctcgacgccg acgtcgtcgt
    49081 cctcgatccg aacggccccg cccgcgccgt ccgcgccacg gagagcgacg cctacgaccc
    49141 cttcccgggc ttcacctcga cactcgccct gcgccacgtc ctcgtccacg gggagcccgt
    49201 cgtgagagac ggcgacctcg tcgacgcgga tgcccgccgg ggacagcttc atccgcagca
    49261 cgactgagca gagaaacagg agagaacgat gccgtcccag acgatccaag acctcgtcac
    49321 cgagctccag ggcctcccga cagacctctt ccagaaggac ttcctcctca cctgggagca
    49381 ccccgaggag cagatccggg cgatcctgac cctcgccgag accttcaagc gcctccacca
    49441 ggagggcacc tcgtatcgcg cgttcgacac gggcctcgcg atctcgatct tccgcgacaa
    49501 ctccacgcgg acccgcttct cgttcgcctc cgccgcgagc gcgatggggc tctccctctc
    49561 cgacctcgac gagcagaaga gccagatcgc ccacggcgag accgtccgcg agaccgcgaa
    49621 catgatctcg ttcctcaccg aggtcatcgg catccgagac gacatgttcc tcggcgccgg
    49681 gaacacctac atgcgcgagg tgggcgcggc cctgaccgac gggacgaagc agggcgtcct
    49741 ccaccgccgc ccgagcctca tcaacctcca gtgcgacgtc gaccacccga cacagtcgct
    49801 cgccgacctc gcctggctga agaagcactt cggcggcctc gagaacctca gggggaagaa
    49861 gatcgccatg acctgggcct acagcccgag ctacggcaag ccgctctccg tcccgcaggg
    49921 gatcatcggc ctgatgaccc gcttcgggat ggacgtgagc ctcgcctacc ccgaggggta
    49981 cggcctgatc ccggaggtcg tcgaggtcgc gaagaagaac gcggccgaag ccggcgggac
    50041 gttcgaggtc gtcaactcga tggaagccgc gttcgcgaac gcggacatcg tctacccgaa
    50101 gtcgtgggcc ccctacgagg tcatgggccg ccggaccgag ctcctgacga agggcgacaa
    50161 ggacggcctg aaggcgctcg agaaggagtg cctcgcgaac aacgcgaagt tcacgagctg
    50221 ggagtgcgac cggaggaaga tggacctgac gaagggtggg aaggcgctct acatgcactg
    50281 cctcccggcc gacatcaccg acgtcagctg caaggcgggc gaggtgagcg ccgaggtctt
    50341 cgagcagtac cggatcccga cctaccacga ggcggcctac aagccgttcg tcatcagcgc
    50401 gctcatgttc ctcgaacgga tcgccgaccc ggccgcgaag ctttccgaga tcgtctcccg
    50461 cggccggaag ctccacatct gaggcccgcc atgtcgagaa tcgtcaagtc gatcgacacc
    50521 gaagtccgca agcgcaccgc gaaacgctgc cgcgagcgcg ggatcgtcat cccgacgtat
    50581 cggcagatgc gggacccgca gctcctcccc gagagcgcga agaagcggct cgagggggtc
    50641 gggctctggg acgtgaaccc cgcgaacctc ttccggatca cctggaagaa cgaccccgtt
    50701 accgggctct tcggcggcgt gaacgccctc gagatcccgc gggagatcac gggggtgaag
    50761 gcgcggatcg tcggcctcgt cgggaagtgg ttcccgaccg gcgcccacaa ggtcggggcg
    50821 gccttctcgt gcctcgtccc gcgcctcgtg agcggcgagt tcgacccgga gaagcacaag
    50881 gccgtctggc cctcgaccgg gaactactgc cgcggcggcg cgttcgactc cgcgatcctc
    50941 ggctgccatg ccgtggcgat cctccccgag aacatgtccc aggagcgctt cacctggctc
    51001 cgcacgatcg gctccgaggt catcgcgacg ccgggctgcg agagcaacgt gaaggagatc
    51061 tacgacaagt gctgggagct gaagaaggac ccggtgcacg tcgtcttcaa ccagttcgac
    51121 gagttcggca acccgatctg gcactacaac gtcaccggcc ccgcggtcga ggacgccttc
    51181 caggccgtcc gcgggccgaa gtcacgcctc tcggcgtggg tgagcgcgac cgggagcgcc
    51241 gggacgatcg ccgcgggcga ctacctgaag cggctcttcc ccgcgatgaa gacggtcgct
    51301 tccgaggcgc tccagtgccc cacgctcctg atgaacggct tcggcgggca ccggatcgaa
    51361 gggatcggcg acaagcacgt cccgtgggtc cacaacgtga ggaacaccga cgccgtcgcg
    51421 gcgatcgacg acgaggactg catgcgcctc ctccgcctct tcaacgagcc ggccggccgg
    51481 gagctcctcg gggcggccgg ggcgagcgcc gacacggtcg ggaagctcga cctcctcggg
    51541 atcagcagca tcggcaacgt cctcgccgcg atcaaggccg cgaagtactg ggagctcgac
    51601 gagaacgacg tcgtcatgac cgtcttcacc gacagcgccg aaatgtacct ctcgcgcctc
    51661 gcggagctga cggccgagcg cggcgccttc accgcggtcg acgccgcccg gacgttcacg
    51721 gggagcctcg agcggcaggc gacggacaac ttcaaggagc tgacctacca ggaccgcaag
    51781 gcgatccaca acctgaagta ctacacctgg gtcgagcagc agggcaagac cttcgaggag
    51841 atcaacgccc agtgggaccc ggagtactgg cgcgagctct tcgaggacga ggtcgtccac
    51901 ttcgaccgcc tcatcgagga gttcaacgcg gaggtcggcc tcggctgacg ccggggccgt
    51961 ccctcgtccg aaggaggacc cgatgcggct cctgatccgg aacggaacgc tcgtcaccct
    52021 cggggaaccc tgccgggtcc tcgaggggca cgatctcctc gtcgagggcg accgcatcgc
    52081 gcggatcgcc cccgccggag agatccccgg ccccttcgac cgcgaggtcg acgcccgcgg
    52141 caaggtcgtc ctccccgggc tcgtcaacgc ccacatgcac ttctactcga cgctcgtgcg
    52201 cggcctcggg aaggccgcgc cgagcgcgaa ctttcaggaa atcctcgaga acctctggtg
    52261 gcgcctcgac cgcaagctct ccctcgacga cgtcgaggtg agcgcgcggg tgatcctcgt
    52321 cgaggcgatc cggaggggga cgacgacgct cgtcgaccat cacgcgagcc ccggcgcggt
    52381 ccgcgggtcg ctcggtcgga tcgcgaaggc cgtgaaggag acggggctcc gctcctgcct
    52441 ctgctacgag gtttccgacc gcgacggcgc aagggtgacg gagcaggggc tcgaggagaa
    52501 cgcggccttc gcccgcgagt gcgccgcggc gagcgacccg cagcttcgcg ccctcttcgg
    52561 tctccacgcc gccttcaccc tctcggacga gacgctcggg aaggccgcac ggatggggcg
    52621 cgacctcggc gtcggcttcc acgtccacgt cgccgaggcg gcctccgacg tcgccgcgaa
    52681 ccgggcgaag cacgggaagt cgtcggcccg gcggctgatc gaccacggca tcctcgggac
    52741 ggggagcatc gccgcccacg ccgtccactg cgacgacgcc gacaaggacg ccctcgccgc
    52801 cagcggcgcg tgggtcgtca cgaaccccca gtcgaacctc aacaacgccg tcggcatcgc
    52861 cgacgtcgtc gggctcgtcc gccggggcgt ccccgtcggc ctcgggaccg acgcgatgac
    52921 cgtcgcgatg ctcgaggagc tccgcgtcgg cctcttcgcg cagcaccttc gccaggagaa
    52981 cccgacctgc ggcttcctcg agctgacggg gacgctgttc gtccagaacc cgcggatcgc
    53041 ctcgaccctc tgggggttcc cgatcggcac gctcgcagag ggggccgcgg ccgacgtgat
    53101 cctcgtcgac tacctcccgc cgaccccgct gaacgacgcg accgccctcg ggcacctcgt
    53161 cttcggcctc tcgcaggccg tcgtcgacac gacgatctgc ggcggccgcg tcctcatgta
    53221 cggccgcagg ctcgagatcg gcctcgacga ggaggccttg gcggccgaga gccgccgcct
    53281 cgcgacagcc ctctgggagc gcttctgagg ccgctccccc gccctcacca cacgacgcgc
    53341 gcggcggtcc gctgcgccga caggaggctc ccgtgacccg tacggccgtc atcgccatcg
    53401 gaggcaactc cctcatcacc gacaaggagc accagacggt gcccgaccag taccaggccg
    53461 cggccgacac gtgcgcgcac atcgcgccgc tcgtcgccgg cggcgacctg cgcctcgtca
    53521 tcacgcacgg gaacgggccg caggtcggct tcatcctccg ccgctccgag ctcgccgccc
    53581 acgagctcca catggtcccg ctcgactcct gtgtcgccga tacgcagggg gcgctcggct
    53641 accacatcca gctcgccctc gggaacgagc tccggagaat cggcgcgacg gcgggaacgg
    53701 cgacggtcgt gacccagtgc ctcgtcgacc ggaccgatcc cgccttcgcg aagccgaaca
    53761 agccgatcgg ctcgttcctg acgaaggagc aggccgagca ccaccgcgag acggacggct
    53821 gggacgtcgt cgaggactcc ggccggggct ggcggcgcgt cgtcgcctct cccgtgccga
    53881 aggcgatcct cgagctcgga gcggtgaaga gcctcctcgg cgccggcttc gtcgtcgtgg
    53941 cggccggcgg cggcgggatc gccgtcgtcg acgacgggac ggggaagctg aagggcgcgg
    54001 ccgcggtcat cgacaaggac cacgcctcga gccttctcgc gcgcgagctc ggggcggacc
    54061 tcctcgtcat ctcgacggcg gtcgagaagg tctgcctgaa cttcggcaag cccgaccaga
    54121 tggacctcga ccggatgacc gtcgccgaag cgaagcggta ctgtgccgag gggcacttca
    54181 agcccgggag catgcttccg aagatccagg cggtcatcgc cttcctcgaa gggggcggcc
    54241 gcgaggcgat catcaccgac ccggcccacc tcggcgaggc gctcgccggg aagaccggca
    54301 cccacgtcgt cccctgagcg gcgctgggcc gagcgggtcc gcgacccggc tcggccgctg
    54361 cgaagtccgc ttcgagcggg cccgcgcagg ccctaaactg gcggctcccg gaggaggccc
    54421 atgtcgacga cgcaggtgga acggccgaag gacgcggccc cgggcggggc ggcggcggcc
    54481 tccttccgcg tgaacggcaa ggaggtcacg gccccgcgcg acgtgaagct cctgacgttc
    54541 ctgcgcgagg agctggacct cacctcggtg aagaacggct gctccgaggg gtcgtgcggg
    54601 gcctgcatgg tcctcgtcga cggcaagccg gcccgttcct gcctcacgac gacggcgaag
    54661 gtcgccggga agagcgtcgt gaccgtcgag gggctcaccg agcgcgagaa ggacgtctac
    54721 tcctgggcct tcgcgaacgc gggcgccgtc cagtgcggct tctgcatgcc ggggatggtg
    54781 atgacggcca aggggctcgt cgacgcgaac cccgagccgg cctccgagga cgtcgcgaag
    54841 gcgctccagc tcagcatgtg ccgctgcacc ggctacgtga agatcgagaa gggaatcctc
    54901 gacgccgccc ggctccttcg cgaagggacc gagccgcccg acgagcacga cgtcccgctc
    54961 tcggtcggcg cgaaggtcct gcgggccgac gcccgcgaga aggtcgtcgg cgcggcgaag
    55021 tacgtcgacg acatgaaggt cccggggatg cttcacgggg ccgtcctccg cccgccgaag
    55081 gcgcgggtga aggtcctcgg catcgacgcg accgaggcgc tcgcgatgcc gggcgtcgtg
    55141 gcggtgatga ccgcggccga cgtcccgggc gaacgcttcc aggggcacat cgtccccgac
    55201 tggccggcga tgatcgcggt gggcgaggag acgcggtacg tgggcgacgc gatcgccctc
    55261 gtcgcggccg agacgcgcga gatcgcgaag gaggcggtga agcgcctccg cctcgacttc
    55321 gaggtcctcc cgcccctcgt cgacgcgcgg aaggctctcg acgacgacgc accgaagctc
    55381 cacccgaagg ggaacctcct cgcgaagacc gtgctgaagc gcggcgaccc cgacgccgcg
    55441 atcgcctccg cggcgcacgt gatccggaag accttccgga cgcccgagac ggagcacgcc
    55501 ttcatggagc ccgagagcgc cctcgccgtc ccgcacgacg acggcacgat gaccgtctac
    55561 accgggacgc agagcatcta cgacgaccac aaggggatcg tcggcgtcct cggcgtcgcg
    55621 cccgagaagg tgaacgtcgt cagcgcctac gtcggcggcg gcttcggcgg caaggaagac
    55681 ctgatcgtcc agcaccacgc cgccctcctc gcctggaaga cgggggcgcc cgtgaaggtg
    55741 acactcgacc gccaggagtc gatccgcgtc cacccgaagc ggcacgcgat ggagatggag
    55801 tacgtcgtgg cggccgacgc cgaggggcgc gtgacggccg tgaaggcgca catcctcgcc
    55861 gacaccggcg cctacgcctc gctgggcggc ccggtcctcc agcgggcctg cactcacggt
    55921 gcggggccgt acacggtccc gaacgtcgag atggtcggga cgggcgtcta cacgaacaac
    55981 ccgcccggag gggcgttccg cggcttcggc gtcacgcagt cgtgcttcgc gatcgagtcg
    56041 tgcctgaacc tcctcgcgaa ggaggtcggc gtcagcccat gggagatccg gtggcggaac
    56101 gccgtcgggc cgggagacgt cctcccgaac gggcaggtct gcgacgacgg gacggcgctg
    56161 aaggagtgcc tcctcgccgt gaaggagctc tgcgagaggc ggcccgacgc ggggctcgcc
    56221 gtcgcgctga agaacgccgg catcggcgtc ggcctccccg acgtcggccg cgtgcggctg
    56281 aaggtcgagg gcggcaaggc cgtgatctac acgagcgccg cctgcatggg gcaggggctc
    56341 gcctcggtcc tgatgcagtt cgtcgccgag atcaccgggc tcggttggga gcaggtcgcc
    56401 gtcgcgccgc ccgacacgtc gacttccccg aacgccggga cgacgacggc ctcacgccag
    56461 acgctcttca cgggcgaggc ggcctgccgc gccgcgcgcg cgctgaaggc cgacctcgac
    56521 tcggcctccc tcgaggcgct cgaggggaag gagtatcgcg ccgagttttc gggcgtgacc
    56581 gaccccttcg cctccgacaa gccgaacccg gtgagccacg tcg
//
LOCUS       DAOONL010000015        52720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_434_length_52720_cov_18.061445, whole genome shotgun sequence.
ACCESSION   DAOONL010000015 DAOONL010000000
VERSION     DAOONL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 52720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_52720_cov_18.061445"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PLP50_04160"
     CDS             <1..1481
                     /locus_tag="PLP50_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA50775.1"
                     /translation="VLVFRALPSEFVPSQDQGRLMLRVQTAVGADLKETDALFRRVES
                     FLLSRPEVTRVFGNVGGFGGAVNSGMIFVSMVDKRDRKMSQAEFSALLRKELNSYPGV
                     RVVVQDPSQSGFTAQRGFPVEFSVRGPDWDVLVEKATEIRDTLSTSGQVVDLDMDYRI
                     GMPELRIVPDRARAADLGISVESVATALNATVGGLRVGKYSSEGRRIDVRLRLLAGQR
                     TRPEDLSRLRLRSASGDMIPLSSLVRTEEVPALQAITRRERERAITLYANVAPGVSQA
                     DALKYVEGLGKDMPTGYRVVLGGASIAFRESMGDLLFALVLGIGVAYMVLASQFNSFL
                     HPVTILSILPLSIAGAAFALLLTGRSLNIFSMIGLLLLMGIVKKNSIVLVDYTNQLRE
                     RGMDTLSALLKAGPIRLRPILMTSIATMMAALPPAIGIGSGSEIRAPMGVAVIGGLIV
                     ATVLSLVVVPSFYLVADRIRERVRGGHADAEAKPSEPAPTPT"
     gene            1609..3525
                     /locus_tag="PLP50_04165"
     CDS             1609..3525
                     /locus_tag="PLP50_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA50776.1"
                     /translation="MIAVSDLGKAFGAQTLFEGVSIQFNPGNRYGLVGANGSGKSTLL
                     RILSGEEAPSNGVVSIPRKLTLGVLSQDHYRYEEMPILDVAMMGNREVWEAMAEKERL
                     LHEDAAFESDRYAELEDVILRHQGYALEARAGEILEGLGIPTEVHRSPLSTLSGGFKL
                     RVLLGQVLAADPGALLLDEPTNHLDIVSIRWLEKFLVSYKGLAIVISHDLRFLDNVCT
                     HIADVDYETVTLYTGNYTAFEAAKEAERERKEAEIQKREKQIAGHQAFVDRFRAKATK
                     ARQAQSKIKLIEKIVIEKLPESSRRRPRFRFRQRRPSGRTALEIESVGKAYGDNVVLK
                     DVSLRVERGDRLAIIGPNGIGKSTLLKIAVGELAPDAGKVTWGYEASPGYFSQDHSEL
                     AGAGRQSIEAWLWEAAPGEPIGFVRGHLGHVLFTGDDAEKPVRALSGGEAARLVFARL
                     AVLGPNVLVLDEPTNHLDIEAIEALVAALLEYDGTLVFVSHDRWFVSRLANRILEISP
                     KGLNDFRGTYDEYLERCGDDHLDAEAVLQSLRKARKSQDVRKKEPEPKRQNRVKALEK
                     KRDELTSLIEAAESRVHEISERFLDPALFGRGAQAETRRLEDEQKQLRKRIEALMVEW
                     EQAERELEAERPGA"
     gene            complement(3529..4713)
                     /gene="argJ"
                     /locus_tag="PLP50_04170"
     CDS             complement(3529..4713)
                     /gene="argJ"
                     /locus_tag="PLP50_04170"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPA50777.1"
                     /translation="MNLPKGFLGSAVAAGLRKKPGLDVALLVAPGGANAAAVFTRNRF
                     QAAPVVLSRASLKKSRRRMKAVVVNAGCANAVTGAEGLAAARRVQERAADLVGCPREE
                     VFVASTGVIGVVLPDEKVSTFLPDAFARLSAGGIEAVSRAILTTDAAPKVARATFRHG
                     GRTCRVLGVAKGAGMIHPNMATMLAFVTTDVPASPALLQAALKEAVDGSFNAISVDGD
                     TSTNDTVLLLASGAAGGPELRSLEGAAPFRAALAKVCNELAWRIVRDGEGARRVMEIT
                     IEGAATEREAKLAAHAVATSQLVKTALYGGDANWGRILAAIGRSGARVSTRRVSLKIG
                     RLSVVEDGAPTGYREATAAKEFARERVPLVADLGVGKARARLLASDLGHEYVAENADY
                     RS"
     gene            complement(4710..6107)
                     /gene="argH"
                     /locus_tag="PLP50_04175"
     CDS             complement(4710..6107)
                     /gene="argH"
                     /locus_tag="PLP50_04175"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPA50778.1"
                     /translation="MSGRRDLWGGRFASGPAEVLRRFNDSWSFDRALIAEDVAGSIAW
                     AEELARSGVLSAAETRMIKKGLREVLAAWEAGEGRDADAEDVHSYVEGELAKRIGPLA
                     GKLHTGRSRNDQVATDLRLWLRGAFDALHAALLDLAAALAARAAEEAATPMPGYTHSK
                     RAEPVTFGHWCLAYVEMLLRDADRVRAARERANECPLGSGALSGTPLPIDRERLARSL
                     GFDRATASSLDGVSDRDGAVEYLFAASLHLSHLSRMAEDLVFFTSDEAGFAELPDALA
                     TGSSRMPQKKNPDLLELARGHAGRAIGELTGLLAVLKGLPLAYDKDLQLDKEPLLRVR
                     GVLEAALPAMTALVPGLKLRRERMRDAATDDRLLTTELADALARRGVPFRQAHEIAGR
                     RFAAAEASGLGILELPPSEGVTEADLASVDLDRALARRGAVGGTSPKRVAQAARRAAA
                     RIARARKAAAKGAGR"
     gene            complement(6104..7360)
                     /locus_tag="PLP50_04180"
     CDS             complement(6104..7360)
                     /locus_tag="PLP50_04180"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPA50779.1"
                     /translation="MSATKIKKAALAYSGGLDTSIIIPWLKENYGCEVVAVAVDVGQA
                     EETSGLKEKALATGACDFHLIDAREEFAAEYLWPVLRAGAVYEHEYLLGTSTARPIIA
                     KKQVEIALQTGCDALSHGCTGKGNDQVRFELAYQALAPELSIVAPWRDWTINSREDAI
                     DYAAARSIPVPVTKKEPYSRDRNLWHISHEGGPLEDPAFEPEESMFKLTVDPRKAPDE
                     PERVTIGFEGGYPVEVNGQLLPAAELLVTLNAIAARHGVGRIDMVENRLVGIKSRGVY
                     ETPGGTLLVQALRALETLTLDRDAMHEKERLALRYAELVYYGQWFSPIREALEAFMEK
                     VTETVTGHVTLKLFKGSAIVVGRRSEHSLYSANLASFNMTGYTAHDATGFIRLFGLPT
                     RGRKRLVPPTDAAVRAGEKLRLEPWG"
     gene            complement(7357..8553)
                     /locus_tag="PLP50_04185"
     CDS             complement(7357..8553)
                     /locus_tag="PLP50_04185"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPA50780.1"
                     /translation="MSRPDESPAAVEARAASYLLGTYSWTPFHPRDGRGAKLVDAEGK
                     VYWDLLAGIAVNSLGYRHPRLVKTLKAEATDLLHVSNLFYQPAQGELAERLVALSGLA
                     RAFFCNSGTEANEAAIKFARLANPGRAKLVALDNSFHGRTVGAVSLTGNLPYREPFEP
                     LLPGVTFLPANDVEALRAAVDDTTSAVFLEPIQGEGGIVPLSPEYLRAARELCDATGA
                     VLVFDEIQCGLGRTGKLFAFQRAGVTPDVVTLAKPLGGGLPLGAVLLNERVAALVKPG
                     HHGTTFGGNPVACRLGLAVLDELVEGGLVAKVEETGAWFGKKLASLAKRCPAVVEARG
                     SGLIRGIELDREAGPVQKALLAKGFVVGTARKTVIRLLPPYVIPKAALSAFLTALEEI
                     LKEGAA"
     gene            complement(8540..9316)
                     /gene="argB"
                     /locus_tag="PLP50_04190"
     CDS             complement(8540..9316)
                     /gene="argB"
                     /locus_tag="PLP50_04190"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPA50781.1"
                     /translation="MTDVVKLGGSLLENRDLRHSALEAIATAWSSGRRSIVVHGGGKR
                     IDAMLAALDIPKKVQGGLRVTDAETLDVVVSILSGLVNKTLVAELRAMGILASGISGA
                     DGDTLWAEFHEAVEGVDLGFVGRVVRCDPKLLSAVLGAGFLPLVASVALGREGTLLNV
                     NADSAASALAGALGARRLVFLTDVEGVLDPEGKVIERLDARAGRALLASPAVTGGMKP
                     KLRAALEAIDAGVSEILVAGPSLHRAALLEGKGGTVLVAA"
     gene            complement(9313..10332)
                     /gene="argC"
                     /locus_tag="PLP50_04195"
     CDS             complement(9313..10332)
                     /gene="argC"
                     /locus_tag="PLP50_04195"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPA50782.1"
                     /translation="MIRVSVVGAAGYSGAEAVRLLSRHPGVELAGLFGSPGGKGALFQ
                     DLHPDLGGASGPDVVPFSEEVLLSGRPDAAILATPNEVSAEVAPRLLATGIRVIDVSG
                     AFRLKDASLYPAWYGFTHPAPSLLAEAVYGLTEWCGDELEGARLVANPGCYPTSVLVA
                     LKPLAALLDPSQPVLVTSASGVSGAGRKSDLAYSFSELAGSYKAYGVGTHRHEPEMRQ
                     ELGLASEAAFTFVPHLLPLVRGILSTIHVTFDAAATDAAIAERYAAAYAGKPFVKVLP
                     AGRLPDLKEVVVTPRAHVGWKLLAGGRRAVVVSAIDNLLKGAASQAVQNLNRVFGRTE
                     TEGLL"
     gene            complement(10370..10864)
                     /locus_tag="PLP50_04200"
     CDS             complement(10370..10864)
                     /locus_tag="PLP50_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPA50783.1"
                     /translation="MPHNHSNASVLRRRDAILRLVREEVVRSQEELQRLLAERGFEAT
                     QPTLSRDVRELGLVKTPGGYALQGTPDRAADAAHRAARLQRTLSEAVLSVRVAGSLVV
                     LRTPPAGAHPVARAVDEADLAEAIGTIAGDDTVFVATPGAVSARALAARLAGPSAVDR
                     RRHA"
     gene            11112..13313
                     /locus_tag="PLP50_04205"
     CDS             11112..13313
                     /locus_tag="PLP50_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPA50784.1"
                     /translation="MTDSTRSELFDRLRAGIVLLVVLAASFALAATASAKPPTDDDCL
                     ACHSEPAGKGKKAPQKALSASVHGEAGLSCVDCHADLARLKQLPHGPKVAPADCTSCH
                     DAVAGSHAFHPMMKDDPTAIACADCHGGHDVVRVADPGSPLAGHAVGKVCATCHDTEV
                     GLYADSAHGRAAASGVKEAPACLACHKTALVSVPDGAPPASAKLAQEKLCLSCHLDNA
                     DVRSRVSPGAGFIAKYEESVHGKALLAGNEKAANCVDCHGAHTVRKKAADGSGPTPLD
                     IEKTCARCHVEVDAAFSKSAHGKALARGVREAPTCTTCHGEHNILRANDPNSPVAAAN
                     VAQKVCTPCHSSLRLSEKYDIKSDAFKTFEESFHGLAVKGGQANVANCASCHGSHAIL
                     PSSDPASRIHKDNLVATCGECHPGAGARFAQGKIHVDTSSKDQEPLLWWIGFIYAGLI
                     VGTIGGMAAHNLLDFVKKSRHQHHVRHGLVEVEPAPHRLYLRMTLEERLQHGALAISF
                     IVLVITGFMLRFPDSWWVVALRNLSSSAFEARSWIHRIAAIVMCLASAYHLYYVIFTA
                     RGRQLVKDLWFRLKDARDLFDMLRYNLGMTPVKPKLDRFSYIEKAEYWALIWGTIVMA
                     ATGFAMWLDNSFMNLFGKLGYDVARSIHYYEAILATLAIIVWHFYFVMFNPDSYPLNT
                     AFWTGHLSHKEMEEEHPLELERILEAEEKERAAAEMAKAQAEAKEREKPKA"
     gene            complement(13378..16527)
                     /locus_tag="PLP50_04210"
     CDS             complement(13378..16527)
                     /locus_tag="PLP50_04210"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HPA50785.1"
                     /translation="MQRPLADGVYDHLLDDALAALVGGVGPEREATIADLEPGRTPAV
                     IAAHLKPLVERVLASVQGEPAERQLRRVEILNRLLATLAEAAREHCTEPPFGEGELRV
                     EGPRHLRLVRTRPALGAAPPVVLPETPLAESALLVNASGEPSLASELSRELATADRVE
                     LLCAFVKWSGLRVLLEALEAVVARGKERGVAAPVRVLTTTYIGASEVRALEALAEIGA
                     EVRISYDDRRTRLHAKAWFFHRESGSHTAYIGSSNLSRAALHEGLEWNVRLSWRDSAP
                     LLSKFRAAFESYWGADEFEPYEPGRDRERVARALAAQRASGGDRGPALRLDLAPHPHQ
                     EPMLEALRVEREHGHWKNLVVAPTGTGKTLVAAFDYRELRAKLPRARLLFVAHRERIL
                     TQSRDAFAAVLRDPGFGELLTSEHEVARGEHVFATIQSLSRRTFAEDLPPEHYDVVIV
                     DEFHHAEAPTYERLLEHVRPRVLLGLTATPERFDGKDVRRWFDGRTAYEMRLWEALEQ
                     GLLAPFQYFGVKDSVDLDDVAWTRGWGYDRGELARRYVVAGGAEARARLVLQEVERRV
                     ADPRRMRALGFCVSVEHARFMAERFAAKGIASEAVVGETPTEDREAAVRRLESGELQT
                     LFTVDLFNEGVDIPTVDTVLFLRPTESATVFLQQLGRGLRLHREKPCLTVLDFIGNAR
                     REFRFDRRFRAILGGTTRQVERQIEAGFPYLPPGCAMQLDPRAMQVVLENIRRSIGSG
                     QRWLAQELSALGPEATVDEFLREAGVEAVELYSGGAVGRSFSSLRHAAFGGAPLNEAT
                     RDRHARLARILHVNDPLRLGFLADLAAGKPLPEGEREERLAAMAAAALLDVRRPAEAR
                     AALEAARADSRYREELGQLVEHLDDSRRDGVLDWRELGPAPLQLHARYRQDEILAALR
                     AGGAEGVPRLQGGVYFEPASGTDVLLVTLRKSERGFSPTTMYRDYAISPSLFHWESQN
                     SAHASTAAGARYVAGTSNVLLFVREVQEQANGVAEAYTFLGRVILESWQGERPMQIVW
                     RLAHPMPGPLYTHATVAAG"
     gene            complement(16940..17278)
                     /locus_tag="PLP50_04215"
     CDS             complement(16940..17278)
                     /locus_tag="PLP50_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50786.1"
                     /translation="MSRPADSAPNDIEVGVREKAAGAGVEVRRGLLEIQVGEEELAPE
                     TDESLREVVVPPLPSLAEPRVPSSVLRDSNGAGADRNAKGGVLREVPGRIETDDEPTD
                     GLNAEEDSGE"
     gene            complement(17727..19181)
                     /locus_tag="PLP50_04220"
     CDS             complement(17727..19181)
                     /locus_tag="PLP50_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPA50787.1"
                     /translation="MPTARERIALWGRTIFRILGQNRLSQAGVVVTTATGISMVFLWV
                     IELLRGGHGHPYSGIILFMILPGLFVAGLLMIPAGYILQRWRDKKAGRAAEPPPPVDF
                     GSPKVRRVATFVLAATLLNATIVGFASYKGLEVMDSVEFCGEACHTVMAPELAAYRGS
                     PHSRIGCVECHIGEGASWFVKSKLSGTRQVFAVAFKTYSKPIPSPVHHLRPARETCEQ
                     CHWPQRFTGDRVVIKKKYAEDEKSTELTTVLVMHVGGHNAQGTVGIHGRHLDAGSRIS
                     YTAIDGRRQEIARVTWKDDTGKEIEFASTEVEATPEQLAKGETRAMDCVDCHNRPTHA
                     FELPHRGLDREITAGSISRDLPFVKKKALELLKVKYASQEEASKAIDAGLRAFYASEH
                     PDVAAAKKAELDRAVKAVQSVYLRNVFPNMDLDWGYHPNNIGHEDFPGCFRCHSGNLE
                     AKDGTTVSADCEACHQVLAMEEENPEILTQLGLK"
     gene            19361..21163
                     /locus_tag="PLP50_04225"
     CDS             19361..21163
                     /locus_tag="PLP50_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPA50788.1"
                     /translation="MARKGRDLTTGRPVRKKLLYFAPSRAHSDSLLGALSAQPGATRT
                     REDGSTVLRAGDADFRFVLRQDPVEAHEALHREYFNGVLLDLRAVPGPASRHEESFAR
                     SLRLLDLMDAEPDPELRFGFHRILALLSGPDSVVVDDRIRQLGARGVGRVLRDPSPGA
                     GERRGPRLPPSAELGRLVVDELLRMTTGRRVGSRALCASGGGITGIYFEMGALKCLAD
                     CLPPGALNAFDMYFGISAGAVLTGILANGYSIDEFMASIAGPGCERIPPCDLSLLKMS
                     HVNLASLAAPLETTTRALAAGLADIVRGRVSPSLSSLFLDYSDFLAAPFRADGYEAML
                     RYLFTRPGATNDFRRLPRRLFVGATDQDAREHVLFGEPGLDDVPISLAIQASLSINPA
                     FSSTKIGERYYVDGAVTRTSDFTEAIRKGADLIFVLDPLVPYVSKGAAGFASRRGVLY
                     NADQDIRTVSYTRFETTRRWVLRQHPEVSLYAFLPANRQRKVLSVNPMDHRPFLAVWR
                     GAYLSTLQRIHLLRHKMCGDLAAHGLALDTAKAEAVAARLEATPEPSFADFFPDGKVA
                     VTKGMKPGRALRLAGAPPNEEVVCRAGERSPAVA"
     gene            complement(21168..22745)
                     /locus_tag="PLP50_04230"
     CDS             complement(21168..22745)
                     /locus_tag="PLP50_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA50789.1"
                     /translation="MPLAEGTRLGNCRLVRKLGEGGMAEVWEAIDLSLERRIAVKVVK
                     ETISSLPEFDARFRREAKTAAQLEHPNILPIYGFGVEEGIAYIEMPYLSGGTLGSRLD
                     AGPPPSPETVCDWVEALASALDAAHARGIIHRDIKAVNVLFDQSGRIVLGDFGLAKNV
                     ADRSGLTATGTLMGTPMYLSPEQARGQSLTPASDQFSLGVLAYRMLAGQLPWGPNAAP
                     AVVMMRIVAQAPPAPSSLRPDLPPAVDAVFERVLAKEPTGRYESCGAFAEALRAALAP
                     SPARAFADAPTVVASQPILVAAPAPAPAPAAPRPRPRPAAPVPPPAPRRSGLVVLLVL
                     FLGVNSVVLALVVWDKVAPKLPWASPAPTETPVPAVAATPTPEPTQDPEPFPIVVVEP
                     TAAAEVATPTPTETPTPEPTPTPTEVPTPTVPPALPPGAIPPVRSSDVRPVFEEELMR
                     RHAGLSGQVELEVLVNADGSVGSIRVVRGVADEVDAVVAEAARQQLAYTPASLKGKPI
                     AAKSRVVVGVRFRVAPR"
     gene            complement(22813..23151)
                     /locus_tag="PLP50_04235"
     CDS             complement(22813..23151)
                     /locus_tag="PLP50_04235"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA50790.1"
                     /translation="MASPPTRSAGVREPSPRGAAGPHLADNMGEVHLLDQPILTIGAA
                     RENDVIVRLPGVSRHHARVLLSGKGTFWLEDLGSRYGSTVNGAVVSRVRLAHGDEIAL
                     GAWRATVNLE"
     gene            23338..24249
                     /locus_tag="PLP50_04240"
     CDS             23338..24249
                     /locus_tag="PLP50_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPA50791.1"
                     /translation="MRFARFVRPVAGLLLVALAPLALDGCVSVGIRRRAAGGSEAGEV
                     AVSVYRKTAERDAGLPVAFPVLSELQKVEDGARTTVARSMASSWSVPGVPPGRYVLRV
                     ARKIDEHGDVVPLKKPYSREFAVAAGERVEAAVVLEKVPVGWIVLAAVTVVLLVVLAI
                     ELAEEVDLPPPPPLPAADLFLRVALVSPWGATARHRAVEPGVADVFPAPGSVVAARRV
                     AVSFLMSRPLDPGAIEADAVLAVGSLSGEIPGDVTWQAGDQLLRFTPSRDFRSGEEVT
                     VTLDLGRVASTDGRSGSGRVSTTFRVP"
     gene            complement(24253..24807)
                     /locus_tag="PLP50_04245"
     CDS             complement(24253..24807)
                     /locus_tag="PLP50_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50792.1"
                     /translation="MKYMEKGGYQNNPLTRLTLWGTFLFLTGLWASNFGMYFSRMGLS
                     PASVVSYYRGSEETFQSARTFGSMVESSHGHFAMMAMVLLVLTHLYIFVPGPRRLKAY
                     FVVGTFGSTFLSEGTGWLVRFVHPAFAALKPIAFLASQAFLALMLGLLAAFLLRAGRQ
                     AALRPVADEEPEAEARRRVAGGAA"
     gene            complement(24815..25417)
                     /locus_tag="PLP50_04250"
     CDS             complement(24815..25417)
                     /locus_tag="PLP50_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPA50793.1"
                     /translation="MSLSRTSSDRTAPSRRRRPRPAAAFFALAALLPGAAGAAVFETQ
                     EQALSRAFPPPARVVRSTAYLTTAQVEAARKAAQAPVESAVVTRYAGFSATGTHLGTA
                     YFDDHVVRTAREVLMIVVAPDGSLRSVDVLAFGEPQDYLPREGWLRRAEGKRSGDGLF
                     VGRALAHVTGATLTTRAIAAAARRILALDAQIGPPARPAK"
     gene            complement(25392..26726)
                     /locus_tag="PLP50_04255"
     CDS             complement(25392..26726)
                     /locus_tag="PLP50_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50794.1"
                     /translation="MNPIPRALVALPICALTLLAGPARSDEPAPAPPPAGSEEAPSNE
                     ELARRIEVLSRELEARRLGDAADPAPLAGSTGKWGLGPAASKVYSKSSGLSIGGYGES
                     LYQNFDATREDGARSGKTNEWDYLRAVVYLGWKFDKTFVLNSEIEYEHASTGKGGEVS
                     VEFLTLDAMLRDELNLRAGLLLAPVGLVNEMHEPTTSLGARRPDTESRILPSTWRANG
                     VGVFGEAGPVAYKLYLTESLSAASFNAANGIRGGRQNGARATADNLAVTGRVDLVSVP
                     GLLAGVSFFSGESGKDLVADGESFGARTTTFDVHADWRFRGLWLRGVFARVSIDDAAL
                     LNRSLGLTGARSIGERQEGYYLSAGYEILSRLAPGTQMALTPFVRWEKTDTQKEVPAG
                     WTRDGANERKAWTVGLDFKPIPQLALKVDWQDYEDAAGTGVPQWNVALAYLF"
     gene            complement(26749..27813)
                     /locus_tag="PLP50_04260"
     CDS             complement(26749..27813)
                     /locus_tag="PLP50_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPA50795.1"
                     /translation="MRRSRGAAVLAPLFLATAGLGAGANEAEDGLEKVRARWLMGTVL
                     EIRLPAGTVDTGGAFEAAFRAVSDVEEVASLWRPGTELGRVHARAAEGATTVVSASLG
                     ALVAEALRVSELTGGAFSPAVGALVTAWDLRGGGRRPTPDERRIAVGRSRSDGVRYDP
                     STRELGLDPGVTLDLDGIAKGFALDRAAEALRARGVDDALLNFGGQILVIGPPVGRPP
                     REAQVASPFDGAAPVLSVRLRDGSLSTSSSSERARMVDGREAGHLLDPRTGELVFATG
                     SVTVLASSGALADALSTAWAVEGPAGFRRAEPVGPFRRSGAVAFVLPGAEGGFETVAD
                     APFVRLRPPSRGPEVTATRP"
     gene            27953..29116
                     /locus_tag="PLP50_04265"
     CDS             27953..29116
                     /locus_tag="PLP50_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPA50796.1"
                     /translation="MANWERRDVVVRTTATGHELTAPVFTARGRQERPVAYVQANVHG
                     GELQGNAAILGLFELLETAPLRGSIVLVPRVNPISANQQVGDYVAGVYDLLTGVNFNR
                     GYLYLTGPSRSASAACYVDVDSFAAAHLSSPIGEIRTGFREALKAALAAIEEEAAAWG
                     ADSRLDFALAIQKMAVDADLVLDLHTGDRAPRYLYSPEGAVAAARAFGLPFVLEVPAR
                     FGGALDEASFVPWQDLSDAFRRLGRDDVPRLVDGFTVELGSMNDFSLDAGREDARRIA
                     SALRYYGVLEGEPDAPLGPITACSASDYRSMHAPVGGLVDLAVRPGTRVKAGDVVVRL
                     ADPSRCRSLPPRAADAVVEVRAPEDGVALLFHAFSSVPKGARLFSMMTRTRPL"
     gene            complement(29187..32108)
                     /locus_tag="PLP50_04270"
     CDS             complement(29187..32108)
                     /locus_tag="PLP50_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HPA50797.1"
                     /translation="MNRKYGLFLASVFAAAALLVPGDSLARPAGDEGIARPAARDVDL
                     RVREPHVGEAMRAGAVVRREPLAAAAEALEEYRDVLGRGPEDGFAFRLEEKDQLGQSH
                     VRMMQTYRGIRVLGGDVVVHLDGDGLLGMSGRFVGGLDLPVVAALAPAESAGAAIAWI
                     EREGGANVEIVDVLDPVVWALSGEPALVVPIRAMWVEDDELRFEDVHVDAATGAFVGS
                     LSKIFTAKNRKIYNMNQACLSTGNELPGTLVINEGGTSSDTAVMGAYDNTGLAYDYYK
                     NIHGRDSYDGSGSVIVSSVHGQFSTGYSCSKNNAAWIDSPYNQMVFGDGDGSTFSNLA
                     RGIDVTTHEFSHAVCSRTADLAYQNDSGALNEANSDILGRAAAFYGGDGNASTRTHWA
                     VGADVYTPGTSGDALRYMYDPAKDGDSADYYPTRNYASGCSPSDSNDYCGVHTNSGIG
                     NLFFYLLSQGGTHPRGKTTVSVTGIGLTKAERIWYRALSVYMTSSTTFQGARTATANA
                     ASDLYGGTCTAEWQSVHKAWDAVGVPGTWSCGTTYSIAGNAGTSGATVSAGGVSTTSD
                     TSGNYTLAGLAAGTYSVTASKTGCTFSPTSISVTVGPSATGRNFTATCSTGDTALTSG
                     VTLTGQSVAKSAWKYYYIDVPSGATSLVFQTTSATADIDIYTRFNAKPTSSTYTCRPY
                     TSSGNETCTATNPSAGRWWLGVYGYAAGSFSVTATVQTGTTTYSIAGNAGTSGATVSA
                     GSKSATSDASGNYSITGLTAGTYSVSATKSGCTFSPTSLSVTVGPNATGKSFTASCGS
                     GGQVERLANGSFESLTASTNSAPDGSWSRTAYSGSSFNTLIANGSAPHGGSDYAYVGV
                     YNSSSQSVTSANVVIPSGATSATLTFYVSIVTSETTTTRAYDKLNVQLVNASTGSVLA
                     TLGTLSNLNKTGSASTYALKSYSVTSYKGQTVKVRFAATNDSSLTTTFRIDDVSLKSD
                     G"
     gene            32439..33086
                     /locus_tag="PLP50_04275"
     CDS             32439..33086
                     /locus_tag="PLP50_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPA50798.1"
                     /translation="MTGGNVATIEFTGNYEDLSTDKGYQFKFFCEKCGNGYMSSFKAS
                     KLGMATSALQAAGNIFGGLLGRAAHGAYEVQRAVGGPAHDAALKDAVAEIRPLFIHCT
                     RCGQWVCGPACFNKKAGLCEGCAPDLDEEMAAAQAEAAREQVQQKAREVDWMKQRDVA
                     TVTGAVCPHCGAKTQGGKFCGDCGKPLATKKKCVTCGTEADGSPKFCPECGGRFS"
     gene            33100..34221
                     /locus_tag="PLP50_04280"
     CDS             33100..34221
                     /locus_tag="PLP50_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50799.1"
                     /translation="MSGVEETRRALCAFETEGIADAPAHGEGEARIDDEGVSVGGFRV
                     EFLDVDVLTDERRVLTLGLHPAGALRISMLARRHETFAKALGEARDSARIRGLLAHGL
                     GVPEAFDGALLEPGPAREARLLVWPTHVAVVPAGADSFQVPLGGVADVRFTEGTWEVV
                     LDAPGGPFHFGRLARRTDAFARSVRAARAAMLERCANASGTRLFADGRAVSAPALASD
                     FERLLEAWSAAERLDGAREIVRRAGREAASIGLAELLDPDEGSLAAKAELPVNTAAFL
                     LAEVRGRVVLELLSGPSAATYVFRGTRAEIGADLAALHFRRRGLALTRAEESGPAGRS
                     YRLALRRLDPLKRLRTATLARVVHDERWSQRLAKALDAT"
     gene            34343..34504
                     /locus_tag="PLP50_04285"
     CDS             34343..34504
                     /locus_tag="PLP50_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50800.1"
                     /translation="MERSAAPRSLASTLVAGGACLPACVLAMSMSMPMCGSSLLSQEE
                     SGYAAGRRM"
     gene            34608..35954
                     /locus_tag="PLP50_04290"
     CDS             34608..35954
                     /locus_tag="PLP50_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPA50801.1"
                     /translation="MSATATVRPALRPGTLAIHAGQESADPATNARAVPIYATTSYVF
                     DDPDHAADLFGLRKPGNIYTRIMNPTTDVFEKRVAALEGGVAAVATSSGQAAETLAIL
                     NLARAGDTIVSTTSLYGGTWALFAHTLPRLGVTTRFVDASGPGGPARVREAIDGTTKA
                     VYVETIGNPRLDVPDLNALADAAHAAGVPLVVDNTFATPLLCRPLEHGADVVLHSATK
                     WIGGHGTAIGGIVVDGGRFDWGASERFRPFFVDPEPAYHGLSFSALAPAAYAARLRTV
                     LLRDVGAAVSPFNSFLFLQGLETLPLRIRRHADNALAVARHLESHPAVAWVRYPGLPS
                     HPTHETAARVLSGGFGGVLTFGVRPPAGVSAEQAARSFIANLSLFSLVANVGDAKSLV
                     IHPWTTTHEQLSEEERLAAGVSPDLVRLSVGIEEPEDLLADLDAALSRLGAPGRTR"
     gene            35951..36718
                     /locus_tag="PLP50_04295"
     CDS             35951..36718
                     /locus_tag="PLP50_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50802.1"
                     /translation="MSARVETLVVKFGGTSLGTPARIRRAARRVAALVASGRRVVAVV
                     SAMGHTTDQILETLGVLEAGRSASAAREVDRALATGEDLSAALLAAALHTLGVPARSL
                     RGGEAGVRADGAFGAARVARVDARRLRALLARGVVPVVSGFQGERSDREVATLGRGGS
                     DTSAVAIAAALGPAPCHIVTDVDAVYDRDPRRHADARPLPRLSHDELLDLARSGAQVV
                     AADAARLAAEHGVPLRVLGYDAAPSDERSGTFVGGAR"
     gene            36715..37770
                     /locus_tag="PLP50_04300"
     CDS             36715..37770
                     /locus_tag="PLP50_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA50803.1"
                     /translation="MNGPPDAAALSAGLSEHRAGDFTLESGETLRDVRQAFRLEGEVD
                     AEGSNVVLLLHSLTGSPADLGGWRPFVGPGLPIDTRRGAVLAPNLLGSCYGTRFHRGT
                     EHGRTPLSITTRDQARLASLLLDALGVQRLALVAGGSLGGMVTLELCATRPDAARAAI
                     VFAAPASPTAWGSAWNHVHRTAIEAAPRSGLALARMVGMLTYRTPLELEQRFRPDGAG
                     PCPVRGYLDHHGEKLARRFTARSYRTLLDAIDSHDVGRGRGSVGAALRPFRGRLVGVG
                     IPGDLLYPPEEVLRWTRAAGAEYRELASQSGHDGFLLETGAVGALLVEALPLGKADRA
                     LAFGPLAAANLGAGPPP"
     gene            37767..39248
                     /locus_tag="PLP50_04305"
     CDS             37767..39248
                     /locus_tag="PLP50_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50804.1"
                     /translation="MIAVRRLVTSPFASLVLAAGLAAGCGKGRFEPAPAALPEGKVWV
                     EHLSRDLAPFWLTPQALGSPVGAFPTFRCDDGSLVDPELPCGEMLVAGPWVSSRLGVE
                     YTRMRSRQTFFYGVAFHLTGEERYLALAKAGVDHLRAHALDRAERSAVSWWKDGIARP
                     PVPARSSQDLAYANLGLAFWAYLTRDPDVLADALLLYEGTFDRYDDPERGLLRWAAGG
                     VGGETARVELVAQLDQVNAYALLLASFAPEPHRSRLRADLARIARVLIDRFWSEELGL
                     FHGSLHDPVERGPGARHTDFGHTVKALWMIERTGALLGDAALSAWARERLPALLERAF
                     LPRHGCWATGLRPDGSLDAGVTWWTAAELDQAAATLALTDRRYTGYLERTWTCWLDRL
                     VDHEDGEVWSWIDLDRPERRGAKQHLWKNGYHSAEHALVSYLVAQELHGRPATLHFAL
                     PDGALPDGLRPYFFDDAAEVTAVTPLRGAPGLRNVAVSFRGIR"
     gene            39298..40041
                     /locus_tag="PLP50_04310"
     CDS             39298..40041
                     /locus_tag="PLP50_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50805.1"
                     /translation="MRTERTGPAAFTVEFESEAELEAEARTNLSAGGLRLPTREKLAI
                     FAKLELLVRLAGGGAVPATATVVAPLPDGFALHVDGDATALAEALRDAPEEPGEEVLE
                     DVAEAEETAVRESVWDRLRGLTRVEKLLLAAKADRVERMILAQDSDPQVLYSLLKNPR
                     LTADEVIRIAKSPFLTFQTAEAMMKSTQWFSNLEVRVALVHNAKTPPAFALRILPTLP
                     ESEVRNISRGAATSMALKVAALKRLQGFT"
     gene            40112..41749
                     /locus_tag="PLP50_04315"
     CDS             40112..41749
                     /locus_tag="PLP50_04315"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPA50806.1"
                     /translation="MPEPAIPSLAEAAAALGLPPERLVPYGHDKAKVLPSPAGFTQRG
                     RLVLVSAITPTGAGEGKTTTSIGLAQGLARIGARASVALREPSLGPTFGMKGGATGGG
                     RARLVPSDDINLHFTGDFHAVTSAHNLLAALLDNHVHHGNRLEIDPRRVFWRRVLDVN
                     DRALRHVVIGLGGVLQGVPRETGFDITPASEVMAALCLAEDAEDLRARLSRIVVALTF
                     GKEPVTAGDLRGAGSMLALLRDALLPNLVRTGEGVPAFVHGGPFANIAHGCSSVIATK
                     TALSLADWVVTEAGFGFDLGAEKFFDIKCAGAGLDTAAVVLVATVRALKRHGGVSKEG
                     LTTPDPAAVERGLPNLAKHLESIRAFGEVPVVALNRFATDSGEEVAVVRRAAEGWGAP
                     FAVCDAFERGGEGAVALAEAVLAHAERKSSPFRPLYAREDPPKEKLAKIARHMYGASS
                     VEWSREAERDLAEIARLGYENLPLCVAKTQMSLSDDPGVVGRPEGFDVTVRGVLLAAG
                     AGYLVPLLGDILRMPGLPSVPQAERIDLVDGKIVGIS"
     gene            complement(41742..43322)
                     /locus_tag="PLP50_04320"
     CDS             complement(41742..43322)
                     /locus_tag="PLP50_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA50807.1"
                     /translation="MTRWPVRLVAAVLLAALLPPAVLVWLSFRLERRALRDDLERRAR
                     SAAALTDALLSSTEGLLSRLGTDWDGGCGAETAESLRSALEVGAGVRGVALLDAELRP
                     VCVAGTAPVPPSPSVRGALGRDARAVWEAGAPGGLIRLALRLPEGSAEAALDAGPIAR
                     ALTELAGGGRDAVEVRGVAGPVLSIPAAPASRRLASPFLLLLGSLVGARDETAVAPCA
                     GGGVSVHLYARREAQLAAWGETLPLLVAIGLGFGAGAAALVLLAGSPGAALTRQLRGA
                     LRRGELEVRYLPIVDVSSGRCVGAEALLRWRHAREGLLSPDLFIALAEETELIVPITE
                     WLMRRVARDLPGGLREPDRFYVAINLAGAHLRDPGTVAAVLRAFAGSRLGPKGLVFEV
                     TEREVIDDVDGTARNVLEALQGWGATIALDDFGTGYSNIATLRRFRLDHLKIDKSFVE
                     GIGTGSVAETVVDTLVELGRKLEMTVVAEGVEREEQVEYLVERGVRHFQGYLFSKPLP
                     LKELVAFVNERAGGPVVS"
     gene            complement(43395..45182)
                     /locus_tag="PLP50_04325"
     CDS             complement(43395..45182)
                     /locus_tag="PLP50_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50808.1"
                     /translation="MTEPAAGTPSERDPATFEEARERLRARGYLDRGVEGAVLKGALA
                     ARTRTRALLRGAALASLLLALALAVVETAAVALSSALSPRDALVLFAWLGAGALVAAA
                     ALVLALAAIAWLRTRGRSDPSRASTEVAVGFGLLAGVGAALAARPVLDAAGPVAAAVA
                     FAAAAAAVVLAVRVARGLAFTVLAASGRSVLSRAPRPALALGTALAAVAVATGGWLLA
                     RRPEPRAEPLVVRAGPARVVLVAVDGWVPELARGALPVPAASRYSYRKETSDPAAFWT
                     TVATGEVARRHGVGALDLVRLDGVSAPVKLTAGTGWYLGRLLPALGVAREESVTSAAR
                     RVPAIWEVAHRGGLASLTVNWWTTWPAGDAGGVVLGNHLYFAARAGGALEGEGWPAEA
                     VRRAATAAPPAAPAADGAQRLVDDARGLDAFAIAAFRGETARSRPRLSLLYLPGLDIL
                     GAALSEPGRSVGERATLAAALAEETRSLASFLAGPDVLGSSAELVVVVLDPGRTGREG
                     ELLVAGPLAAEGASGEVRPIDLAPTLLAVLGVPASRQAEGRVDSRIVRAGSATTATVG
                     SWGARPRGVAAPIDPKEYVESLRSLGYLR"
     gene            complement(45223..46122)
                     /locus_tag="PLP50_04330"
     CDS             complement(45223..46122)
                     /locus_tag="PLP50_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPA50809.1"
                     /translation="MSISLPETLARVRRAVDDRLPALLSAQPSGAGAPAVGDALPTAA
                     REALLSPGKRVRPGLVFLVGELFGAPEERLLDPACAVEMIHAASLALDDLPSMDDATT
                     RRGRPALHAVHGEDLAILAAVTLIVRAFGVLADAGLSALRPATALDLVSRLADATGLL
                     GLASGQSLDLRTPEAAASFDRLETIHARKTGALFVASAEFGAVLGGARERELAAVRSY
                     ARNVGLAFQIVDDLLERQDPARTGKHANRAPAPTFARHVGEEGARRLVAELTAHAVEA
                     LEPFGAKGALLREFASLLGARTA"
     gene            46189..49386
                     /locus_tag="PLP50_04335"
     CDS             46189..49386
                     /locus_tag="PLP50_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA50810.1"
                     /translation="MPAASTKPSSRAREWLSALLAPAVVLLGFAALALTRGPSPLRVL
                     TTGLLTAAAVAALGRLSAGRLLRSREATRDALHRASAGDLTLSRAALARSGENALAEA
                     LHGLMVAMERILASFTRLSAAVSGVAVDLTTRGRELSLAAAMQSSRAEETADAMRGTD
                     AAIGSLRESMETLAGAAENAGASLHEMTASVTEVSRSTTALRAFVDETTASLGGVVSA
                     LQQVASAVENLSGLADETARATAAIRSSTAETDRQAQTGARLSERVSAAVVSGKTAVS
                     GTLAGMHEIRAAVSGASDASTALAEHSARIGEILHAIEEIAGETNLLALNASIIAAQA
                     GESGKTFSVLADDIRDLSERTAVSTEEVRGLVSSVRGGVEDVRRLLADARRRTEEGVD
                     LAQTSDATLDEIEALALESRRASEGIAGMAVAQSADAARVSEATGRVSAEVGRISEAT
                     RGQLETTRTVHARADRVRELTEQLARAMEEQAVGGQTLLGSMERVTSTVDAIAEATVT
                     LADGSAAVVGSMDEIRRAARQNAYAATSMNQAAQALEQESLLLRSRSSLFRLPSPAPG
                     GRIRAALRYLDEANFDPAFSQTVPQAILARTWGEGLVKFGEGTRIVPELAERWEIDPT
                     GTLFTFHLRRGVRWHEGGTLSAADVKASFERFLSPSTGAPLAALFDAVEGYEDFRAGR
                     SPGVAGLEAPSGSVLAVRLSRPVPFFLQLLTLPDITVLPPAILGDPERIRRFPCGTGA
                     FVPREIAFGKSARFDRFDGYWERNRIAPDGIDLALAEDSEAGVFQRFLDGQLDVVWDI
                     PYPEAARLMGDPAWRPYIDSSVQLHTSFLVLRCDRAPLDDVRVRRALNYAVDRAALNE
                     RTFAGLSIPAASILPPHVVGHDPALRPFRHEPETARALLREAGHGDGLTLSAWLSPKD
                     ARDPLNPLPAIATQLEAVGVNLALEVLPGEEAAARKRNGVFPDVRLSRWFADFPDPDT
                     FFRSLFSSKAESVLDGGYANPEVDRLVEKGARLLDAAEREALYRELDRLVQSEAPLVF
                     LFHNRGFVAHAPRLRGVQAHLLLPPVRWNELWLEP"
     gene            complement(49476..49823)
                     /locus_tag="PLP50_04340"
     CDS             complement(49476..49823)
                     /locus_tag="PLP50_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPA50811.1"
                     /translation="MNKNASIRKAGEITIIELKGKITIGAGDLQMREAIHAALAEGAR
                     KLVVDMSQVVTIDSSGVGELVGCFTTATHKGAKMKLMNLPAKISDVLTVTQLITVFDV
                     YPNEAEAIASFNG"
     gene            complement(49820..50275)
                     /locus_tag="PLP50_04345"
     CDS             complement(49820..50275)
                     /locus_tag="PLP50_04345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50812.1"
                     /translation="MPAAAPGPIVLWLSSRYENIELAQAALAHVCRLRGIDAEMEHWI
                     GMAMREAVANAVKHGNRLDPDKRVLVRLESAGDELTIVIGDEGGGFDPERVSDPLAPE
                     NQLKTSGRGIFYIRTFMDDVTFSRGEGGGTVLTMRKDLSKRGNAKGEKQ"
     gene            50355..51647
                     /locus_tag="PLP50_04350"
     CDS             50355..51647
                     /locus_tag="PLP50_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50813.1"
                     /translation="MCAPEPCDDVAEIIRDPGRRRPLGKPLSPVVGLLVLAALAAPPA
                     RSDDSALLAVRVARAQTLVEKVRGAAFRAPVASALLPEKQLETVLAKKLVQDLPIPFE
                     AYAAGLAALGLIEPSPGLLDRLIRLYTRQVVGFYDPGEKRFYIVPERSRDVAGPAGEL
                     MEQLLLAHELTHALQDQRLGLDRRMKALRDSTDALLALQAFLEGEATVLMTEALLESV
                     PEEAREALGADPLGQVLDGLDDPDGVDGAEGVPAYFVRELVFPYAAGTAWVRQKKAAG
                     GWPAVDAAYRRLPTTTREILRPGVVLPARLRLAPADRPTPRAVPGGGTASWADTLGEW
                     VLGTLLEQAGAGDESRDAAAAWQDDRIVFSCPGKSPGAHGVGFLWRIRAATPAGAARI
                     AELLGPLYATRPAPARPRISVRGDLVEVVRSVVPPPPA"
     gene            complement(51651..52430)
                     /locus_tag="PLP50_04355"
     CDS             complement(51651..52430)
                     /locus_tag="PLP50_04355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPA50814.1"
                     /translation="MKPIDLVLLLGVGLPIVAAIVVVAARRGAFRRDEFPHDRRRAGA
                     LVALGVTLAATTILPAFSLGRPIDPARLSLPSILALQQLLLFFLVAWWLLAGRPPLAE
                     FLALTSASPRREVRTGLVVGLAGWGLTVAAGLVVLGVLRFAGVDEVRPIPPLVRWLAS
                     QPFASRLVLVAAAMTVEEVFFRSFLQRRLGAVPASILFLVSHGGYGDPLYFVGLLAIT
                     TVLAVTFERTGSVLAPMVAHGTFDAVQLLLVLPSAVRAIPG"
BASE COUNT         7283 a        19563 c        18619 g         7255 t
ORIGIN      
        1 cggtcctcgt cttccgcgcg ctcccgtcgg agttcgtccc gtcgcaggac cagggccgcc
       61 tcatgctccg ggtccagacc gccgtaggcg ccgacctcaa ggagaccgac gcgctcttcc
      121 ggagggtcga atccttcctc ctgtcccgcc cggaggtgac gcgcgtcttc gggaacgtcg
      181 gcggcttcgg cggggccgtc aacagcggga tgatcttcgt ctcgatggtc gacaagcggg
      241 accggaagat gtcgcaggcg gagttctcgg ccctcctccg gaaggagctg aactcctatc
      301 cgggcgtccg ggtcgtcgtg caggacccct cgcagtccgg cttcacggcc cagcgcggat
      361 tccccgtcga gttttccgtc cgcggtcccg actgggacgt cctcgtcgag aaggcaaccg
      421 agatccgcga cacgctctcg acgagcggtc aggtcgtcga cctcgacatg gactaccgga
      481 tcgggatgcc ggagctccgg atcgtcccgg accgcgcccg cgccgcagac ctcggcatca
      541 gcgtcgagag cgtcgcgacg gcgctcaacg cgacggtcgg agggctccgg gtcgggaagt
      601 actcctccga agggcgccgg atcgacgtcc gcctgaggct cctcgccggg cagcggacgc
      661 gccccgagga cctctcgcgc ctgcgcctga ggagcgcctc gggcgacatg atcccgctct
      721 cctccctcgt caggaccgaa gaggtcccgg cgcttcaggc gatcacccgg cgtgagcgcg
      781 agagggcgat caccctctat gccaacgtcg cccccggcgt ctcgcaggcc gacgcgctga
      841 agtacgtcga agggctcggg aaggacatgc cgaccggcta ccgcgtcgtc ctcggcgggg
      901 cgagcatcgc cttccgcgag tcgatgggcg acctcctctt cgccctcgtc ctcgggatcg
      961 gcgtcgccta catggtcctc gcctcgcagt tcaactcgtt cctccacccc gtgacgatcc
     1021 tgtcgatcct tccgctctcg atcgccggag ccgccttcgc gctcctgctg accgggcgga
     1081 gcctgaacat cttctcgatg atcgggctcc tcctgctgat ggggatcgtc aagaagaact
     1141 ccatcgtcct cgtcgactac acgaaccagc tccgcgagcg cggcatggac acgctgagcg
     1201 cgctcctgaa agcgggtccg atccgcctca ggcccatctt gatgacctcc atcgcgacga
     1261 tgatggccgc cctgccgccc gcgatcggca tcggctccgg ctccgagatc cgggcgccga
     1321 tgggcgtggc cgtgatcggc ggactcatcg tggcgacggt cctctcgctc gtcgtcgtcc
     1381 cctcgttcta cctcgtcgcc gaccggatcc gggagcgggt ccgcggcggc cacgcggacg
     1441 cggaggcaaa gccgtcggag cccgcgccga cgccgacctg accgccgtcc tgcccgggcg
     1501 agatccgaga ggtccggcgc gctcctcgcg agcgtgccgg accgactccc gtcccgcacg
     1561 ccaaccgggc cgagcgccct tccgggcgtc tgctatggtc cccgccccat gatcgccgtc
     1621 tcggatctcg gaaaggcctt cggcgcgcag accctcttcg agggcgtctc gatccagttc
     1681 aaccccggaa accgttacgg cctcgtcggg gcgaacggct cggggaagtc gacgctcctg
     1741 cgcatcctct cgggagagga ggccccgtcg aacggcgtcg tctcgatccc ccggaagctg
     1801 acgctcggcg tcctttccca ggaccactac cgctacgagg agatgccgat cctcgacgtc
     1861 gcgatgatgg ggaaccggga ggtctgggag gcgatggcgg agaaggagcg gctcctccac
     1921 gaggacgccg ccttcgagag cgaccgctac gccgagctcg aggacgtcat cctccggcac
     1981 caggggtacg cgctcgaggc gcgggcgggc gagatcctgg agggcctcgg gatcccgacg
     2041 gaggtccacc gctcccctct ctcgacgctc tccggcgggt tcaagctgcg cgtcctcctc
     2101 gggcaggtgc tcgcggccga cccgggcgcg ctcctcctcg acgagccgac gaaccacctc
     2161 gacatcgtct cgatacgctg gctcgagaag ttcctcgtct cctacaaggg cctcgcgatc
     2221 gtcatctccc acgacctccg attcctcgac aacgtctgca cccacatcgc cgacgtcgac
     2281 tacgagacgg tgacgctcta caccggcaac tacaccgcgt tcgaggccgc gaaagaggcg
     2341 gagcgggagc ggaaggaagc ggagatccag aagcgcgaaa agcagatcgc cgggcaccag
     2401 gcgttcgtcg accgcttccg cgcgaaggcc acgaaggctc gccaggcgca gagcaagatc
     2461 aagctcatcg agaagatcgt catcgagaag ctccccgaga gctcccgacg gcgcccgcgg
     2521 ttccggttcc gccagcggcg gccgagcggc cgcaccgccc tcgagatcga gtccgtcggc
     2581 aaggcgtacg gcgacaacgt cgtcctgaag gacgtctcgc tccgggtcga gcggggcgat
     2641 cgcctcgcga tcatcggccc gaacgggatc ggcaagtcga cgctcctgaa gatcgcggtg
     2701 ggcgagctcg cgcccgacgc cggcaaggtc acctggggct acgaggcgag ccccggctac
     2761 ttctcccagg accacagcga gctcgccggc gccggacggc agtcgatcga ggcgtggctc
     2821 tgggaggcgg cgcccggcga gccgatcggc ttcgtgcgcg gacacctcgg ccacgtcctc
     2881 ttcaccggcg acgacgcgga gaagcccgtc cgcgccctct ccgggggcga ggccgcgcgc
     2941 ctcgtcttcg cccgcctcgc ggtcctcggt ccgaacgtcc tcgtcctcga cgagccgacg
     3001 aaccacctcg acatcgaggc gatcgaggcg ctcgtcgccg ctctcctcga gtacgacggc
     3061 actctcgtct tcgtctccca cgaccgctgg ttcgtctcgc gcctcgcgaa ccggatcctc
     3121 gagatctcgc cgaagggact caacgacttc cgggggacgt acgacgagta cctcgagaga
     3181 tgcggcgacg accacctcga cgcggaggcc gtcctccaga gcctccggaa ggcccggaag
     3241 agccaggacg tgaggaagaa ggagcccgag ccgaagcgcc agaaccgcgt gaaggccctc
     3301 gagaagaaac gcgacgagct gacgagcctc atcgaggccg cggagtcgcg ggtccacgag
     3361 atcagcgagc ggttcctcga cccggcgctc ttcggaaggg gcgcccaggc cgagacgcgc
     3421 cggctcgagg acgagcagaa gcagctgcga aagaggatcg aggctttgat ggtcgagtgg
     3481 gagcaggccg agagggagct cgaggcggag cgcccgggcg cgtaggcttc agctccggta
     3541 gtccgcgttc tccgccacgt actcgtggcc gaggtccgag gcgaggaggc gcgcccgcgc
     3601 cttccccacg cccaggtccg cgacgagagg gacccgctcg cgggcgaact ccttcgccgc
     3661 cgtggcctcc cggtagccgg tcggggcgcc gtcctcgacg acggagagac gaccgatctt
     3721 cagcgagaca cgacgcgtcg agacgcgcgc gccgcttcgg ccgatcgcgg cgaggatgcg
     3781 gccccagttc gcatcgccgc cgtacagggc cgtcttcacg agctgagagg tcgccacggc
     3841 gtgcgccgcg agcttcgcct cgcgttcggt ggcggctccc tcgatcgtga tctccatgac
     3901 ccgccgcgcc ccttcgccgt cgcggacgat ccgccacgcg agctcgttgc agaccttcgc
     3961 cagggctgcg cggaagggtg cggccccttc gagggaccgg agctccggcc cgcccgcggc
     4021 gcccgacgcg aggaggagga ccgtgtcgtt cgtcgaggtg tcgccgtcga ccgagatcgc
     4081 gttgaagctg ccgtccaccg cctccttcag ggccgcctga aggagcgccg gcgaggccgg
     4141 aacgtcggtc gtcacgaagg cgagcatcgt cgccatgttc gggtggatca tccccgcacc
     4201 cttggccacg ccgaggacgc ggcaggtccg cccgccgtgg cggaaggtcg cgcgggcgac
     4261 cttcggggcc gcgtccgtcg tcaggatggc gcgcgagacg gcctcgatgc cgccggcgga
     4321 gagccgcgcg aacgcgtcgg ggaggaacgt cgagaccttc tcgtcgggga ggacgacgcc
     4381 gatcacgccg gtcgaggcga cgaagacctc ctcccgggga cagccgacga ggtcggccgc
     4441 gcgctcctgg acgcgccgag cggccgcgag cccctccgcg cccgtcaccg cgttcgcgca
     4501 gccggcgttc acgacgacgg ccttcatgcg cctgcggctc ttcttcagcg aggcgcgcga
     4561 gaggacgacc ggcgccgcct ggaagcggtt cctcgtgaag acggcggccg cgttcgcgcc
     4621 tccgggggcg acgaggaggg ccacgtcgag gcccggcttc ttccggaggc cggccgcgac
     4681 ggccgagccg agaaagccct tcggcaggtt catcgtcccg ctcctttcgc ggcggccttc
     4741 ctcgccctgg cgatccgcgc cgcggccctg cgcgccgcct gagcgacgcg tttcggcgaa
     4801 gtcccgccca ccgccccgcg gcgcgcgagc gcccgatcga ggtcgaccga agcgaggtcc
     4861 gcctcggtga cgccttccga cggcggtagc tccaggatcc cgaggcccga cgcttccgcc
     4921 gccgcaaacc gccgcccggc gatctcgtgg gcctgccgga acgggacgcc ccggcgcgcg
     4981 agcgcgtcgg cgagctccgt cgtcaggagc cggtcgtccg tcgcggcgtc gcgcatccgc
     5041 tcgcggcgga gcttcaggcc cggcacgagc gccgtcatcg ccgggagggc cgcctcgagg
     5101 acgccgcgca cccggaggag cggctccttg tcgagctgga ggtccttgtc gtaggcgagc
     5161 gggagcccct tgagaacggc gaggaggcct gtgagctcgc cgatcgcgcg cccggcgtgc
     5221 ccgcgcgcga gctcgaggag gtcggggttc ttcttctgcg gcatccgcga ggagccggtg
     5281 gcgagggcgt cgggcagctc ggcgaagccc gcctcgtccg acgtgaagaa gaccaggtcc
     5341 tcggccatcc gcgagaggtg cgagaggtgg agcgaggcgg cgaagaggta ctcgacggcc
     5401 ccgtcgcggt cggagacgcc gtcgagggag ctcgccgtcg cgcggtcgaa gccgaggctc
     5461 cgcgcgaggc gctcccggtc gattggaagt ggcgtccccg agagcgcgcc cgagccgagc
     5521 gggcactcgt tcgcgcgctc tcgcgccgcg cgcacccggt ccgcgtcgcg caggagcatc
     5581 tcgacgtagg cgaggcacca gtgcccgaag gtcacgggct cggcccgctt gctgtgcgta
     5641 tagcccggca tcggcgtggc ggcttcctcg gccgcccgcg cggcgagggc cgcggcgagg
     5701 tcgaggagcg cggcgtggag ggcgtcgaag gcgccccgca gccagaggcg gaggtcggtc
     5761 gcgacctggt cgttccgcga gcggcccgtg tgaagcttcc ccgcaagcgg gccgatccgt
     5821 tttgcgagct cgccctcgac gtacgagtgg acgtcctcgg cgtcggcgtc gcgcccttcg
     5881 cccgcctccc acgccgcgag gacctcccgg agacccttct tgatcatccg ggtctccgcc
     5941 gccgacagga cgcccgatcg cgcgagctcc tccgcccagg cgatcgagcc ggcgacatcc
     6001 tccgcgatca gggcccggtc gaacgaccac gaatcgttga accggcggag gacctccgcc
     6061 ggcccggagg cgaaccgccc tccccagagg tcgcggcgcc cactcatccc cacggctcga
     6121 ggcggagctt ctcgcccgcc ctcacggccg cgtccgtcgg cgggacgagg cgcttgcgac
     6181 ctcgcgtcgg caggccgaag aggcggatga agcccgtcgc gtcgtgggcc gtgtagcccg
     6241 tcatgttgaa ggaggcgagg ttcgccgagt agagcgagtg ctcggagcgg cggccgacga
     6301 cgatggccga gcccttgaag agcttcagcg tgacgtggcc ggtgacggtc tccgtcacct
     6361 tctccatgaa cgcctcgagc gcctcgcgga tggggctgaa ccactgcccg tagtagacga
     6421 gctcggcgta gcggagggcg aggcgctcct tttcgtgcat cgcgtcgcgg tcgagcgtga
     6481 gcgtctcgag ggcgcggagg gcctggacga ggagcgtccc accgggagtc tcgtagacgc
     6541 cgcgcgactt gatgccgacg aggcggttct cgaccatgtc gatccgcccg acgccgtgcc
     6601 gcgccgcgat ggcgttcagc gtgacgagga gctctgcggc cgggaggagc tgcccgttca
     6661 cctcgaccgg gtagccccct tcgaagccga tcgtcacgcg ctccggctcg tcgggcgcct
     6721 tccgcgggtc gaccgtcagc ttgaacatcg actcctcggg ctcgaacgcc gggtcctcga
     6781 gcgggccgcc ctcgtgcgag atgtgccaga ggttccggtc gcgcgagtag ggctccttct
     6841 tcgtcaccgg gaccgggatg ctccgggcgg cggcgtagtc gatcgcgtcc tcgcgggagt
     6901 tgatcgtcca gtcgcgccac ggcgcgacga tcgaaagctc cggcgcgagc gcctggtagg
     6961 cgagctcgaa gcggacctgg tcgttcccct tccccgtgca gccgtgggag agggcgtcgc
     7021 agcccgtctg aagcgcgatc tcgacctgct tcttcgcgat gatcggccgc gccgtcgagg
     7081 tgccgaggag gtactcgtgc tcgtagacgg ccccggcgcg gaggacgggc cagaggtact
     7141 cggccgcgaa ctcctccctc gcgtcgatca ggtggaaatc gcacgcgccg gtcgcgagcg
     7201 ccttctcctt caggcccgag gtctcctcgg cctgccccac gtcgaccgcg accgccacga
     7261 cctcgcagcc gtagttctcc ttgagccagg ggatgatgat cgacgtgtcg aggcccccgg
     7321 aataggcgag ggcggccttc ttgatcttcg tcgcgctcat gcggcacctt ccttcaggat
     7381 ctcttcgagt gcggtcagga aggccgagag ggcggccttc ggaatgacgt acggcgggag
     7441 gaggcggatc acggtcttgc gcgccgtgcc gacgacgaag cccttcgcga ggagcgcctt
     7501 ctgcaccggg cccgcctcgc ggtcgagctc gatcccgcgg atgaggccgg agccgcgcgc
     7561 ttccacgacg gccgggcacc gcttggcaag cgaagcgagc ttcttcccga accaggcgcc
     7621 cgtttcctcg accttcgcga cgaggcctcc ctcgaccagc tcgtcgagga cggcgaggcc
     7681 gagccggcag gcgacgggat tgccgccgaa cgtcgtcccg tggtggccgg gcttcacgag
     7741 cgcggcgacc cgctcgttca ggaggacggc gccgagcggg agcccgccgc cgagcggctt
     7801 ggccagcgtg acgacgtcgg gcgtgacccc ggcccgctgg aacgcgaaga gcttccccgt
     7861 ccgcccgagg ccgcactgga tctcgtcgaa gacgaggacg gcgcccgtcg cgtcgcagag
     7921 ctcgcgcgcg gcgcggaggt actcgggcga gagcgggacg atccccccct cgccctggat
     7981 tggctcgagg aagaccgccg acgtcgtgtc gtccacagcc gcgcggaggg cctcgacgtc
     8041 gttcgccggg aggaaggtca cgccggggag gagcggctcg aacggctcgc ggtagggcag
     8101 attaccggtc agcgagaccg ccccgaccgt ccgcccgtgg aacgagttgt cgagcgcgac
     8161 gagcttcgca cggccgggat tggcgaggcg cgcgaacttg atcgccgcct cgttcgcctc
     8221 ggtgccgctg ttgcagaaga aggcgcgcgc gaggcccgag agcgccacga gccgttcggc
     8281 gagctcgccc tgggccggct ggtagaagag gttcgagacg tggaggaggt ccgtcgcctc
     8341 ggccttcagc gtcttcacga ggcgcgggtg gcggtagccg agggagttca cggcgatgcc
     8401 ggcgaggagg tcccagtaga ccttcccctc cgcgtcgacg agcttcgccc cgcgcccgtc
     8461 gcgcgggtgg aacggcgtcc aggagtaggt gccgaggaga tacgaagccg cgcgagcctc
     8521 gaccgccgcg ggggattcgt caggccgcga caaggaccgt ccctcccttc ccctcgagga
     8581 gggccgcccg gtggagcgac ggccccgcga cgaggatctc ggagacgccg gcgtcgatcg
     8641 cctcgagcgc agcgcggagc ttcggcttca tgccgcccgt cacggcgggc gaggcgagga
     8701 gcgcgcggcc ggcccgggcg tccagccgct cgatgacctt cccctccgga tcgaggaccc
     8761 cctcgacgtc cgtcaggaag acgaggcgcc tggcgccgag cgccccggcc agggccgagg
     8821 cggcggaatc ggcgttcacg ttcaggagcg tcccctcgcg accgagcgcg acggaggcca
     8881 cgagcggcag gaagcccgcg ccgaggacgg ccgagaggag cttcgggtcg cagcggacga
     8941 cccggccgac gaagccgagg tcgaccccct cgaccgcctc gtggaactcg gcccagagcg
     9001 tgtcgccgtc cgccccggag atcccggagg cgaggatccc catcgcgcgg agctcggcga
     9061 cgagcgtctt gttgacgagc ccggagagga tcgagacgac gacgtcgaga gtctccgcgt
     9121 cggtgacacg cagcccgccc tgcaccttct tcgggatgtc gagagcggcg agcatcgcgt
     9181 cgatccgctt gcccccgccg tggacgacga tcgagcgcct ccccgagctc caggcggtgg
     9241 cgatcgcctc gagggcggag tgccggaggt cgcggttctc gaggaggctt ccgcccagct
     9301 tgacgacgtc ggtcaaagga gtccctccgt ttcggtccgt ccgaagaccc ggttgaggtt
     9361 ctggaccgcc tgcgaggcgg cccccttgag gaggttgtcg atcgccgaga cgacgaccgc
     9421 gcgccggcct ccggcgagga gcttccagcc gacgtgggcc cgcggcgtga cgacgacctc
     9481 cttcaggtca gggaggcgcc ccgcggggag gaccttcacg aacggcttcc ccgcgtaggc
     9541 ggccgcgtat cgctccgcga tcgccgcgtc ggtcgcggcc gcgtcgaacg tgacgtggat
     9601 cgtcgagagg attccgcgga cgagcgggag gaggtgcggc acgaacgtga acgccgcctc
     9661 cgacgcgagc ccgagctcct ggcgcatctc cggttcgtgc cggtgcgtcc cgacgccgta
     9721 ggccttgtag cttccggcga gctcgctgaa ggagtatgcg aggtcggact ttcttcccgc
     9781 tcccgagaca ccgctcgccg aagtgacgag aacgggctgc gacgggtcga ggagcgccgc
     9841 gagcggcttg agggcgacga ggaccgaggt cgggtagcag cccgggttcg cgacgagcct
     9901 cgcgccctcg agctcatccc cgcaccactc cgtgaggccg tacacggcct ccgcgaggag
     9961 cgacggggcc gggtgcgtga atccgtacca cgccgggtag agagaggcgt ccttcagccg
    10021 gaacgcgccc gagacgtcga tcacccggat ccccgtggcg aggagcctcg gcgcgacctc
    10081 ggccgagacc tcgttcggcg tcgcgaggat cgccgcatcg ggacgacccg aaagaaggac
    10141 ctcctccgag aacgggacga cgtcggggcc ggaggctccg ccgagatcgg ggtggaggtc
    10201 ctggaagagc gcgcccttcc ctcccgggct tccgaagagc ccggcgagct cgacgcccgg
    10261 atggcgcgag aggaggcgga ccgcctccgc ccccgagtag cccgcggcgc ccacgacgga
    10321 gacacgaatc acgatcgaat cctcgattct ccggagcggc gtccggactc taagcgtggc
    10381 gtcggcggtc gaccgcggac ggaccggcga ggcgggcggc gagggcgcgc gcggagacgg
    10441 cgccgggcgt cgcaacgaag accgtgtcgt cgccggcgat cgtcccgatc gcctccgcga
    10501 ggtccgcctc atcgacggcc cgcgccaccg ggtgtgcacc ggccggcggc gtcctgagga
    10561 cgacgagaga gccggcgacg cggacggaca ggaccgcctc cgagagtgtc cgctgcagcc
    10621 gggccgcccg gtgcgccgcg tccgccgccc ggtcgggagt cccctgaaga gcgtagcccc
    10681 ccggggtctt tacgaggccg agctcccgga cgtccctgga gagggtcggc tgcgtcgcct
    10741 cgaaccctcg ctcggccagg aggcgctgga gctcctcctg cgaccggacg acctcctccc
    10801 ggacgagccg gaggatggcg tcccgacggc ggaggaccga cgcattcgaa tgattatgcg
    10861 gcacgaagaa ataatattca ccaagtcgcc gcggcgtcaa gccctcccgg gaacggtcgc
    10921 ggattggccg acgacccggc gcttcgcccc gcttccggcc gtagccgccg ctccccacgg
    10981 ctccccgcgg gctcgagggg ccggcaggcc gcttcgccgc gagaacacat ctcaggtccg
    11041 gccccaactg atgcctctga atcattgaag aggcccctca cggtccgaat aatccccgcc
    11101 cgggggtact cgtgaccgac agcacccgtt ccgagctctt cgaccggctt cgcgccggca
    11161 tcgtcctcct ggtcgtcctg gcggcctctt tcgcgctcgc ggccaccgcc tcggcgaagc
    11221 cgccgacgga cgacgactgc ctcgcctgcc actccgagcc ggcgggcaag gggaagaagg
    11281 cgccgcagaa ggcgctctcc gcctccgtcc acggcgaggc cgggctctcg tgcgtcgact
    11341 gccacgcgga cctcgcgagg ctgaagcagc tccctcacgg cccgaaggta gcgcccgccg
    11401 actgcacctc ctgccacgac gctgtcgccg ggagtcacgc cttccatccg atgatgaagg
    11461 acgacccgac ggcgatcgcc tgcgccgact gccatggcgg gcacgacgtc gtccgggtcg
    11521 ccgaccccgg gtcgccgctg gccggccacg cggtggggaa ggtctgcgcc acgtgccacg
    11581 acaccgaggt cgggctctac gccgactcgg cccacggccg tgccgccgcg tccggcgtga
    11641 aggaagcccc cgcctgcctc gcctgccaca agaccgcgct cgtctccgtt cccgacggcg
    11701 ccccgcccgc gagcgccaag ctggcccagg agaagctctg cctctcctgc cacctcgaca
    11761 acgcggacgt ccgctcgcgc gtctctccgg gagccggctt catcgccaag tacgaggaga
    11821 gcgtccacgg caaggccctc ctcgcgggga acgagaaggc cgcgaactgc gtcgactgcc
    11881 acggggcgca caccgtccgg aagaaggcgg ccgacggcag cggccccacc ccgctcgaca
    11941 tcgagaagac ctgcgcccgc tgccacgtcg aggtcgacgc ggcattctcg aagagcgccc
    12001 acggcaaggc cctcgcccgc ggcgtgcggg aagcgccgac ttgcacgacc tgccacggcg
    12061 agcacaacat cctgagggcg aacgacccga actcccccgt cgccgccgcg aacgtcgcgc
    12121 agaaggtctg caccccctgc cactcctccc tccgactctc cgagaaatac gacatcaaga
    12181 gtgacgcctt caagacgttc gaagagagct tccacggcct cgccgtgaag ggaggccagg
    12241 cgaacgtcgc gaactgcgcg agctgccacg gctctcacgc gatcctcccc tcctccgacc
    12301 ccgcctcccg gatccacaag gacaacctcg tcgcgacctg cggcgagtgc cacccgggcg
    12361 ccggcgcccg tttcgcgcag gggaagatcc acgtcgacac ctcttcgaag gaccaggagc
    12421 cgctcctctg gtggatcggc ttcatctacg cgggcctgat cgtcgggacg atcggcggca
    12481 tggcggccca caacctcctc gacttcgtga agaagtcgcg gcatcagcat cacgtccgcc
    12541 acggcctcgt cgaggtcgag ccggcgccgc accgcctcta cctgcgcatg acgctcgagg
    12601 agcgcctcca gcacggggcc ctcgcgatca gcttcatcgt cctcgtcatc acgggcttca
    12661 tgctccggtt ccccgactcg tggtgggtcg tcgcgctgcg gaacctctcc tcctcggcct
    12721 tcgaggcccg gagctggatc caccgcatcg ccgcgatcgt catgtgcctc gcgtcggcct
    12781 accacctcta ctacgtcatc ttcaccgcgc gcgggcggca gctcgtcaag gacctctggt
    12841 tccggctgaa ggacgcccgc gacctcttcg acatgctgag gtacaacctc ggtatgaccc
    12901 cggtgaagcc gaagctcgac cgcttcagct acatcgagaa ggccgagtac tgggccctca
    12961 tctgggggac gatcgtcatg gccgccacgg gcttcgccat gtggctcgac aacagcttca
    13021 tgaacctctt cgggaagctc ggctacgacg tcgcccggtc gatccactac tacgaggcga
    13081 tcctcgcgac gctcgcgatc atcgtctggc atttctactt cgtgatgttc aaccccgact
    13141 cctacccgct gaacacggcc ttctggaccg gccacctctc gcacaaggag atggaggagg
    13201 agcacccgct cgagctggag cggatcctcg aggcggagga gaaggagcgg gcggcggcgg
    13261 agatggccaa ggcgcaggcc gaggcgaagg aacgggagaa gccgaaggcc tgatcctcac
    13321 gcagtcaacg gtgaacccgg agggacccgg tccgcgagga ccgggtccct cttcgcttca
    13381 gcctgccgcc acggtcgcgt gcgtgtagag ggggcccggc atcgggtgag cgaggcgcca
    13441 gacgatctgc atggggcgct cgccttgcca cgattcgaga atcacccgcc cgaggaacgt
    13501 ataggcctcc gccacgccgt tcgcctgctc ctgcacctcg cggacgaaga ggaggacgtt
    13561 gctcgtcccg gccacatacc tcgctccggc cgcggtgctg gcgtgggccg agttctggct
    13621 ctcccagtgg aagagcgacg gcgaaatcgc gtagtcgcgg tacatcgtcg tcggcgaaaa
    13681 gcccctctcc gacttccgga gcgtgacgag gaggacgtcg gtgccgctcg cgggctcgaa
    13741 gtagacgccc ccctgaaggc gcgggacgcc ctcggcccct cccgcccgca gcgcggcgag
    13801 gatctcgtcc tgccggtagc gcgcgtggag ctggaggggc gccggtccga gctcgcgcca
    13861 gtcgagcact ccatcgcggc ggctgtcgtc gaggtgctcg acgagctgcc ccagctcctc
    13921 ccggtatctc gaatccgccc gggccgcctc gagcgccgcg cgggcctccg cgggtcgccg
    13981 gacgtcgagg agggccgccg ccgccatcgc cgcgagccgc tcctcccgct ccccttccgg
    14041 caacggcttc cccgcagcga ggtccgcgag gaagccgagc cggagagggt cgttcacgtg
    14101 gaggatgcgg gccaggcgcg catggcggtc gcgggtcgcc tcgttcaggg gcgctccgcc
    14161 aaatgcggcg tgccggagcg aagaaaagct ccgcccgacg gcgccgccag agtagagctc
    14221 caccgcctcg acgcccgctt cccgaaggaa ctcgtcaacg gtcgcctccg gcccgagggc
    14281 cgacagctcc tgcgcgagcc aacgctgccc tgagccgatg ctcctgcgga tgttctcgag
    14341 gacgacctgc atcgccctcg ggtcgagctg catcgcgcag ccgggcggca ggtacgggaa
    14401 tcccgcctcg atctgccgct ccacctgccg tgtcgttccg ccgagaatcg cgcggaacct
    14461 cctgtcgaac cggaactcgc gccgggcgtt cccgatgaag tcgaggaccg tcagacacgg
    14521 cttttccctg tgcaggcgca gcccgcgccc gagctgctgg aggaagaccg tcgcgctctc
    14581 ggtgggccgc aggaagagga cggtgtcgac cgtcgggatg tccactcctt cgttgaagag
    14641 gtccaccgtg aagagggtct gcagctcgcc cgactcgagc cggcggacgg ccgcctcccg
    14701 atcctccgtc ggcgtctcgc cgacgacagc ctcgctcgcg atccccttcg ccgcgaaacg
    14761 ctcggccatg aagcgggcgt gctccaccga gacgcagaac ccgagcgccc gcatccgccg
    14821 cgggtcggcg acgcgacgct cgacctcctg gaggacgagc cgcgctcgcg cttccgcgcc
    14881 gccggccacg acgtagcgcc gcgccagctc tccccggtcg tagccccacc cgcgcgtcca
    14941 cgccacgtcg tccaggtcga ccgagtcctt cacgccgaag tactggaacg gagcgaggag
    15001 tccctgctcc agggcctccc agaggcgcat ctcgtatgcc gtccgcccgt cgaaccagcg
    15061 gcgcacgtcc ttcccgtcga agcgctccgg cgtcgccgtc aggccgagaa ggacgcgggg
    15121 ccgcacgtgc tcgaggagcc gctcgtaggt cggcgcttcg gcgtggtgga actcgtcgac
    15181 gatgacgacg tcgtagtgct ccggcggcag gtcctccgcg aacgtcctcc gcgagagcga
    15241 ctggatcgta gcgaaaacgt gctctccgcg ggcgacctcg tgctcgctcg tcagcagctc
    15301 gccgaagccc gggtctctca ggacggccgc gaaggcgtcg cggctctgcg tcaggatccg
    15361 ctcgcggtgg gcgacgaaga ggagcctcgc ccgcggcagc ttcgcccgca gctcgcggta
    15421 gtcgaacgcc gccacgagcg tcttgccggt ccccgtcggc gcgacgacga ggttcttcca
    15481 gtgaccatgc tcccgctcga cccgcagcgc ctccagcatc ggctcctggt gcgggtgggg
    15541 cgcgaggtcg agcctcagcg ccgggccccg gtcccctccg gacgccctct gcgccgccag
    15601 tgcccgcgcg acccgctcgc gatcgcggcc cggctcgtac ggctcgaact cgtcggcgcc
    15661 ccagtacgac tcgaaggccg cccggaactt cgagaggagc ggggccgaat cacgccacga
    15721 aagcctcacg ttccactcga gcccctcgtg gagcgcggcg cgggagaggt tcgaggaacc
    15781 gatgtaggcc gtgtggctac cgctctcccg gtggaagaac cacgccttcg cgtggaggcg
    15841 cgtccggcgg tcgtcgtacg agatccgcac ctcggcaccg atctcggcga gcgcttcgag
    15901 cgcccggacc tcgctcgcgc cgatgtacgt ggtcgtcagg acgcgcaccg gggcggccac
    15961 cccacgctcc ttcccccgtg cgacaacggc ctccagcgcc tcgagcagca ctctgagccc
    16021 gctccacttc acgaacgcgc agaggagctc cacgcggtcc gccgtcgcca gttccctcga
    16081 aagctcgctc gcgagcgacg gctcgcccga ggcgttcacg aggagcgcgc tctcggcgag
    16141 gggtgtctcg ggcagcacca ccggaggcgc ggctccgagt gcgggccgtg tccggacgag
    16201 gcggaggtgc cgcggtccct cgacccgcag ctccccctcg ccgaacggcg gctccgtgca
    16261 gtgctcgcgg gccgcctccg cgagcgtcgc gaggagtcgg ttcaggatct ccacccggcg
    16321 gagctgccgc tccgccggct cgccctgtac cgaggcgagg accctctcga cgagcggctt
    16381 cagatgtgcc gcgatgacgg ccggagttcg gcccggttcc aggtccgcga tcgtcgcctc
    16441 acgctcgggc ccgacgcctc cgacgagcgc cgcgagcgcg tcgtcaagga ggtgatcgta
    16501 gacgccgtca gcgagcggcc tctgcaccgc gccaggttac tcgagcggat ggccgcgccg
    16561 ccaggcggac agagtccgaa ttcgctcccg ggcgagcgat cacgcccgcg gcacgaggga
    16621 gctccctttc ccccgggagc tcgatagcct cgcccggatg gcgagttctc gatccgttcc
    16681 gtccggaccc aaaggctcgc cacttctcga ccatcttcgc ggcacactct gtcgctccgg
    16741 ttgccggttt ctcgggatcg agggaacgga ccaggacggc tttcggctct cattcgagga
    16801 ccgcctgctc aggcgctcgg gcatccgcgg cttcctcttc cgactggggc ccgccggcgc
    16861 cgcccgaaac cgcctcgtca cgacggttcg actcccggac tccttcgaga gtcgacgcgg
    16921 cgctcgagac cccttcggtc tattcaccac tgtcctcctc ggcattgagc ccgtccgtgg
    16981 gctcgtcgtc ggtttcgatc cttcccggca cttcccggag gacgcccccc ttcgcgtttc
    17041 gatcagcgcc ggcgccgttc gaatcacgca ggaccgagga tggcactcgt ggctccgcga
    17101 gggatgggag cggcggaacg acgacttcgc ggaggctctc gtcggtttcc ggagcgagct
    17161 cctcctcacc tacctggatt tcgagcaaac cgcggcggac ctcgacaccg gcccccgcag
    17221 ccttctcgcg gacacctacc tcgatgtcgt tcggcgccga gtccgcagga cgtgacaccc
    17281 accgcgagca tcgccgctcc caagaaaagc accgccttgc gcgccccccc ggcatcccgg
    17341 gagggcgcgg acgatgagcc aggcccacac cgcgagcgtc cggccggtgc gtcccgcgag
    17401 cgttcgagca cgtgcgggga cccgaacggg tccggcgagc aggccgacgg ggtgagcggc
    17461 cgcactgtct gacgagcgga gcgaggagtt tgcggccgcc cgtcggcccg cgcaggcggg
    17521 cccgattcgg gtcgcgcgcg cgcagagcga gccgcgggat gcaccggccg ggcgcggacc
    17581 acgaagacgc gagctgccct cggacggact cgactcggga ttctcggcag aatgcgccgg
    17641 ccccgcccga tacgtgggca ccgggcgggg ccgcgtcgtc tgtggcaggc gccgaccttc
    17701 gcgccgacgc ctgcccttcc ggccccctac ttcagcccga gctgcgtgag gatctccggg
    17761 ttctcctctt ccatggcgag gacctggtgg cacgcctcgc agtcggcgga gaccgtcgtc
    17821 ccgtccttcg cctcgaggtt cccgctgtgg cagcggaagc agccggggaa gtcctcgtgc
    17881 ccgatgttgt tcgggtggta gccccagtcg aggtccatgt tcgggaagac gttccggagg
    17941 tagacggact ggacggcctt cacggcccgg tcgagctcgg ccttcttcgc ggccgcgacg
    18001 tccgggtgct cggacgcgta gaacgcccgg aggcccgcat cgatcgcctt cgacgcctcc
    18061 tcctgggagg cgtacttcac cttcaggagc tcgagggcct tcttcttgac gaacggcagg
    18121 tcgcggctga tcgacccggc ggtaatctcg cgatcgaggc cgcggtgcgg gagctcgaag
    18181 gcgtgggtcg ggcggttgtg gcagtcgacg cagtccatcg cccgggtctc gcccttcgcg
    18241 agctgctcgg gggtcgcctc gacctcggtg ctcgcgaact cgatctcctt gccggtgtcg
    18301 tccttccagg tgacgcgggc gatctcctgg cggcgcccgt cgatcgccgt gtagctgatg
    18361 cgcgagccgg cgtcgaggtg gcggccgtgg atgccgaccg tcccctgcgc gttgtgcccg
    18421 ccgacgtgca tgacgaggac ggtcgtcagc tcggtgctct tctcgtcttc cgcgtacttc
    18481 ttcttgatga cgacgcggtc cccggtgaag cgctgcggcc agtggcactg ctcgcacgtc
    18541 tcgcgcgccg gccggaggtg gtgcacgggc gacgggatcg gcttcgagta ggtcttgaag
    18601 gcgaccgcga agacctggcg cgtgcccgag agcttcgact tgacgaacca ggaagcccct
    18661 tccccgatgt ggcactcgac gcagccgatg cgggagtgcg gcgagccgcg gtaggccgcg
    18721 agctccgggg ccatcacggt atggcaggcc tcgccgcaga actccacgga gtccatcacc
    18781 tcgagcccct tgtaggaggc gaagccgacg atcgtggcgt tcaggagcgt cgcggcgaga
    18841 acgaaggtcg cgacgcgccg gaccttcgga gagccgaagt cgaccggcgg gggcggctcg
    18901 gcggcccggc cggccttctt gtcgcgccag cgctggagga tgtagcccgc ggggatcatg
    18961 aggagcccgg ccacgaagag gccgggcagg atcatgaaga ggatgatccc ggagtaggga
    19021 tgcccgtggc ctccgcggag cagctcgatc acccagagga agaccatcga gatcccggtc
    19081 gccgtcgtga cgacgacgcc cgcctggctg agccggttct gtccgaggat cctgaagatc
    19141 gtccgccccc agagggcgat tcgttcgcgc gcggtcggca tctggcctct cctcctccgg
    19201 cgtagtttca cacgggaaga ccccggagcc ggccggtccc gccgagagtc cgcgacccgc
    19261 gccccggcag gacggagcgg cttgacgcct cgcggcaaat ggcggagatt gccggaaggg
    19321 cggatcgagc ggccgccgcg gcacggcgag ggggtgcagc gtggcgagaa aagggcggga
    19381 cctgaccacg ggacggccgg tccgcaagaa gctgctctac ttcgccccga gccgcgccca
    19441 ctccgacagc ctgctcgggg cgctttcggc gcagcccggc gcgacccgta cccgcgagga
    19501 cgggtcgacc gtccttcgag caggcgatgc cgacttccgt ttcgtcctca ggcaggaccc
    19561 ggtcgaagcc cacgaggcgc ttcaccgcga gtacttcaac ggggtcctcc tcgaccttcg
    19621 cgcggttccg ggacccgcct cacggcacga ggagagcttc gcccgctccc tccgcctcct
    19681 cgacctgatg gacgccgagc ccgacccgga gcttcgcttc ggcttccatc gcatcctcgc
    19741 cctcctctcg ggacccgact ccgtcgtcgt agacgaccgc atccgtcagc tcggcgcccg
    19801 cggcgtcggg cgggtcctcc gggacccctc ccccggcgca ggcgagcgcc gcggtccccg
    19861 cctcccgccc tcggccgagc tcggacgcct cgtcgtcgac gagctcctcc ggatgacgac
    19921 cggccgccgc gtcggatcga gggcgctctg cgcctccggg ggcgggatca ccggcatcta
    19981 tttcgagatg ggcgcgctga agtgcctggc cgactgtctc ccgcccggcg ccctgaacgc
    20041 cttcgacatg tacttcggga tcagcgcggg cgcggtcctg acggggatcc tcgcgaacgg
    20101 ctactcgatc gacgagttca tggcttccat cgccggcccc ggctgcgagc gcatccctcc
    20161 ttgcgacctc tcgctcctga agatgtcgca cgtcaacctc gcgagtctcg cggcgccgct
    20221 ggagacgacg acgcgggcgc tcgcagccgg cctcgcggac atcgtccgcg gacgggtcag
    20281 cccgtcgctg tcgtcgctct tcctcgacta ctcggacttc ctcgcggcgc cgttccgcgc
    20341 agacggctac gaggcgatgc tccgctacct cttcacccgg cccggggcca cgaacgactt
    20401 ccgcaggctg ccgcgccgcc tgttcgtcgg agcgaccgac caggatgccc gcgagcacgt
    20461 cctcttcggc gagcccggcc tcgacgacgt gccgatcagc ctcgcgatcc aggcgtcgct
    20521 cagcatcaac ccggcgttct cgtcgacgaa gatcggagag cgctactacg tcgacggcgc
    20581 cgtcacccgg acgtccgact tcaccgaggc gatccggaag ggggcggacc tgatcttcgt
    20641 cctcgacccg ctcgtcccgt acgtctcgaa gggggctgcc ggcttcgcga gccgccgggg
    20701 ggtcctctac aacgccgacc aggacatccg gaccgtctcg tacacgcgat tcgagacgac
    20761 ccggcgctgg gtcctccgcc agcatcccga ggtgagcctc tacgcgttcc tcccggcgaa
    20821 ccgccagagg aaggtcctct cggtgaaccc gatggaccac cgtccgttcc tcgcagtgtg
    20881 gcggggcgcc tacctctcca cgctccagcg gatccacctc cttcgccaca agatgtgcgg
    20941 ggacctcgcc gcccacggcc tggcgctcga cacggcgaag gcggaggcgg tggccgcgcg
    21001 gctggaagcg accccggagc cttcgttcgc ggacttcttc cccgacggga aggtcgccgt
    21061 cacgaagggg atgaagccgg gccgggcgct tcggctcgcg ggcgcccctc ccaacgagga
    21121 ggtcgtctgc cgggcggggg aacggagccc ggccgtcgcc tgaggtgtca gcggggcgcg
    21181 acgcggaatc ggacgcccac gacgacgcgg ctcttcgccg cgatcggctt ccccttgaga
    21241 gaggccggcg tgtatgcgag ctgttgccgg gccgcctcgg cgacgaccgc gtcgacctcg
    21301 tcggcgaccc cccgaacgac gcggatcgat ccgacgctcc cgtccgcgtt caccaggacc
    21361 tcgagctcga cctgtccgct caggccggcg tggcgccgca tcagctcctc ttcgaagacc
    21421 gggcggacgt ccgaggagcg gaccggaggg atcgctcccg gggggagcgc cggcgggacg
    21481 gtcggcgtcg gcacctcggt cggtgtcggg gtcggctcgg gcgtcggcgt ctcggtcgga
    21541 gtcggcgtcg ccacttccgc ggccgccgtc ggctcgacga cgacgatcgg aaagggttcc
    21601 gggtcctgcg tgggctccgg cgtcggcgtg gcggcgacgg ctggcaccgg cgtctcggtc
    21661 ggggccggag aggcccaggg cagcttcggc gcgaccttgt cccagacgac gagcgccagg
    21721 acgacggagt tgacgccgag gaagaggacc aggaggacga cgaggcccga tctccgcggg
    21781 gcgggaggag gaaccggcgc ggcaggacgc ggcctcgggc ggggcgctgc gggcgccgga
    21841 gccggagcgg gcgccgcgac gaggatgggc tgcgacgcga cgacggtcgg cgcgtcggcg
    21901 aaggcgcggg cgggtgacgg cgcgagcgcc gcccggaggg cctcggcgaa ggccccgcag
    21961 ctttcgtagc gtccggtcgg ctccttcgcg aggaccctct cgaagacggc gtcgaccgcg
    22021 ggcgggaggt ccggccggag cgacgagggc gcgggcggcg cctgcgcgac gatccgcatc
    22081 atcacgacgg ccggggccgc gttcggcccc cacgggagct ggcccgccag catccggtag
    22141 gcgaggacac cgagcgagaa ctggtccgag gccggcgtca gcgactgccc gcgcgcctgc
    22201 tccggggaga ggtacatcgg cgtccccatg agggtcccgg tcgcggtcag ccccgatcgg
    22261 tccgcgacgt tcttcgccag gccgaagtcg ccgaggacga tccgtccgct ctggtcgaag
    22321 aggacgttca cggccttgat gtcgcggtgg atgatcccgc gcgcgtgcgc cgcgtcgagc
    22381 gcggacgcga gcgcctcgac ccagtcgcag acggtctcgg gcgacggcgg cggccccgcg
    22441 tcgaggcgcg atccgagcgt ccctcccgag aggtacggca tctcgatgta ggcgatcccc
    22501 tcctcgacgc cgaagccgta gatcgggagg atgttcgggt gctcgagctg ggccgcggtc
    22561 ttcgcctcgc gccggaagcg cgcgtcgaac tccggcaggc tcgagatcgt ctccttgacg
    22621 accttcaccg cgatgcggcg ctcgagcgag aggtcgatcg cttcccagac ctcggccatc
    22681 ccgccctcgc ccagcttccg gacgaggcgg cagttcccca gtcgtgtccc ttcggccagc
    22741 ggcatcccgg cggtcgtcct cccgtatcgg tcgagcgatg ttagtcgatc ggccgcggcg
    22801 cggccggcgg cgctactcga ggttcacggt ggcgcgccac gcccccaggg cgatctcgtc
    22861 gccgtgggcg agccggacgc gcgagacgac ggctccgttc accgtcgatc cgtagcggct
    22921 gccgaggtcc tcgagccaga aggtcccctt tccggagagc aagacgcgag cgtggtgccg
    22981 cgagacgccg ggcagccgga cgatcacgtc gttctcgcgg gccgcgccga tcgtcaggat
    23041 cggctggtcg aggaggtgga cctctcccat gttgtccgcc aggtgagggc cggccgcgcc
    23101 gcggggcgac ggttccctca cgccggccga cctcgtgggg ggggacgcca tggcgcaagg
    23161 atacgcgccc ggggccgatg gtgcaccatc ggagcgaatc cagaccgcga ccggtccggg
    23221 aacgcgcgat tcgaccgggt tcccgccgag cggcgggccc cctggggcgc tgcggcggac
    23281 gacgcgccgc tcggcacggt tgacacggct ccgcgcgcgg gacgagcatc ccgcctgatg
    23341 cgcttcgccc gtttcgtccg cccggtggcc gggctattgc tcgttgccct cgcaccgctc
    23401 gcactcgacg gctgtgtctc ggtcggaatc aggcggcgcg ccgccggcgg ctcggaggcg
    23461 ggcgaggtgg ccgtttccgt ctaccggaag acggccgagc gcgacgccgg gctcccggtc
    23521 gcgttccccg tcctttccga gctgcagaag gtcgaggacg gcgcacgcac gaccgtcgcg
    23581 cgctcgatgg cgtcctcgtg gtccgtcccc ggggtgccgc caggccgcta cgtccttcgc
    23641 gtggcgcgga agatcgacga gcacggcgac gtcgtgccgc tgaagaagcc gtactcccgg
    23701 gagttcgccg tcgcggccgg cgagcgcgtc gaggccgccg tcgtcctcga gaaggtcccc
    23761 gtcggctgga tcgtcctcgc ggccgtcacg gtcgtcctgc tcgtcgtcct cgccatcgag
    23821 ctcgccgagg aggtcgacct cccgccgccg cctccgctgc cggccgccga cctcttcctg
    23881 cgcgtcgcgc tcgtctctcc ctggggcgcg accgcgcgcc acagggccgt cgagccgggc
    23941 gtggccgacg tcttccccgc gccgggctcg gtcgtcgccg cacgccgcgt cgccgtgagc
    24001 ttcctgatgt cgcggccgct cgatccgggc gcgatcgaag cggacgccgt cctcgccgtc
    24061 ggctccctct ccggcgagat cccgggggac gtgacgtggc aggccggcga ccagctcctc
    24121 cggttcaccc cctcccgcga cttccggtcc ggggaggagg tcacggtgac gctcgacctc
    24181 ggccgggtcg cgtccacgga cggtcggagc ggcagcggcc gcgtttcgac gacgttccgc
    24241 gtcccctgag gctcaggccg ctccgcccgc caccctccgc cgcgcttcgg cctcgggctc
    24301 ctcgtccgcg accgggcgaa gcgccgcctg tcgccccgcc cgaaggagga aggctgcgag
    24361 aaggcccagc atgagggcga ggaacgcctg cgaggcgagg aaggcgatcg gcttgagggc
    24421 tgcgaacgcc gggtgaacga accggacgag ccagcccgtc ccctcggaga ggaacgtcga
    24481 gccgaacgtg ccgacgacga agtacgcctt cagcctgcgc ggcccgggaa cgaagatgta
    24541 gaggtgggtc aggacgagga ggaccatcgc catcatcgcg aagtgcccgt gggacgactc
    24601 caccatcgag ccgaacgtgc gcgccgactg gaacgtctcc tccgacccgc ggtaatacga
    24661 gacgacgctc gccggggaga gccccattcg ggagaagtac atcccgaaat tggaggccca
    24721 gagccccgtc aggaagagga acgtccccca gagcgtgaga cgggtgagcg ggttgttctg
    24781 gtaccccccc ttctccatgt acttcatggt ctcctcactt cgccggcctg gccggcgggc
    24841 cgatctgcgc gtcgagcgcg aggatgcgtc gagccgccgc cgcgatcgcc cgggtcgtca
    24901 gcgtggcgcc ggtgacgtgg gcgagcgccc gtcccacgaa gagcccgtcg ccggaccgct
    24961 tcccctccgc ccgccggagc catccctcgc gcgggaggta gtcctgcggc tcgccgaagg
    25021 cgaggacgtc gacgctccgc aggctgccgt cgggcgcgac gacgatcatc aggacctcgc
    25081 gggccgtcct cacgacgtgg tcgtcgaagt aggccgtccc gaggtgcgtc ccggtcgccg
    25141 aaaaccccgc gtagcgagtg acgaccgccg actcgaccgg tgcctgtgcg gccttccgcg
    25201 ccgcttccac ctgggccgtc gtcaggtacg ccgtggagcg gacgacgcgg gccggcggcg
    25261 ggaacgcgcg ggagagcgct tgctcctgcg tctcgaagac ggcggcgccc gccgcgcccg
    25321 ggaggagcgc ggcgagcgcg aagaaggccg ccgccggccg aggccgacgg cggcgcgagg
    25381 gcgccgtccg atcagaagag gtacgcgaga gcgacattcc actgggggac gccggttccc
    25441 gcggcgtcct cgtagtcctg ccagtccacc ttgagcgcga gctgcgggat cggcttgaag
    25501 tcgagcccga cggtccacgc cttcctctcg ttcgccccgt cgcgcgtcca gcccgcgggg
    25561 acctccttct gcgtgtcggt cttctcccac ctcacgaacg gcgtcagcgc catctgcgtg
    25621 ccgggagcga gacgcgagag gatctcgtag cccgcggaga ggtagtaccc ctcctgccgc
    25681 tcaccgatcg agcgcgcgcc cgtcaggccg agcgaccggt tcagcagcgc ggcgtcgtcg
    25741 atcgagacgc gcgcgaagac gccccgcagc cagagcccgc ggaaccgcca gtcggcgtgc
    25801 acgtcgaacg tcgtcgtccg ggcgccgaac gactctccgt cggcgacgag gtccttcccc
    25861 gattcgcccg agaagaacga gacccccgcg aggagccccg gcacggagac gaggtcgacg
    25921 cgtcccgtca cggcgaggtt gtcggccgtc gcccgggcgc cgttctgccg ccccccccgg
    25981 atgccgttcg cggcgttgaa cgacgccgcg gagaggctct ccgtcagata gagcttgtag
    26041 gcgaccggcc ccgcctcgcc gaagacgccg accccgttcg ccctccaggt cgaggggagg
    26101 atccggctct cggtgtcggg gcggcgggcg ccgagggagg tcgtcggctc gtgcatctcg
    26161 ttcacgaggc cgaccggcgc gaggaggagg ccggcccgaa ggttcagctc gtcgcgcagc
    26221 atcgcgtcga gggtcaggaa ctcgaccgag acctcgcccc ccttccccgt cgaagcgtgc
    26281 tcgtattcga tctcgctgtt caggacgaag gtcttgtcga acttccagcc gagatagacg
    26341 accgcgcgga ggtagtccca ctcgttcgtc ttccccgacc gggcgccgtc ttcgcgcgtc
    26401 gcgtcgaagt tctggtagag cgactcgccg tagcctccga tcgagaggcc ggagctcttc
    26461 gaatagacct tcgaggcggc ggggccgagg ccccacttgc cggtggaccc ggccagcggg
    26521 gcgggatccg ccgcgtcgcc caggcggcgc gcttcgagct cgcgcgagag gacctcgatg
    26581 cgccgggcga gctcctcgtt ggagggggcc tcctcggaac cggccggcgg gggcgccggc
    26641 gcgggctcgt cggagcgggc ggggcccgcg agaagggtca gggcacagat cgggagcgcg
    26701 acgagcgctc tcggaatcgg gttcatcgat ctctcctctg aggtggattc aaggtctggt
    26761 cgcggtgacc tcggggccgc gcgacggcgg ccggagtcgg acgaaagggg catcggcaac
    26821 cgtctcgaac ccgccttccg cgcccggaag gacgaacgcg accgcgcccg agcgccggaa
    26881 cggccccacg ggctcggcgc gtcggaaccc ggccggcccc tcgacggccc acgccgtcga
    26941 gagggcgtcc gccagggcgc cggacgaggc gagaacggtc accgatcccg tggcgaagac
    27001 gagctctccc gtccggggat cgaggaggtg ccccgcttcc cgcccgtcga ccatccgcgc
    27061 gcgctcggag ctcgacgacg tggagaggga cccgtcccgg agccggacgg agaggaccgg
    27121 agcggcgccg tcgaacggcg acgcgacctg cgcctcgcgg ggcggccggc cgacgggcgg
    27181 accgatcacg aggatctgcc caccgaagtt caggagggcg tcgtcgacgc cccgggcccg
    27241 gagcgcctcc gccgcccgat cgagggcgaa tcccttcgcg atgccgtcga ggtcgagcgt
    27301 gacgcccggg tcgagaccga gctcgcgcgt cgacgggtcg tagcggacgc cgtccgaccg
    27361 ggagcgcccc acggcgattc gccgttcgtc cggcgtgggc cgtcgcccgc cgccgcgaag
    27421 gtcccacgcg gtcaccaagg ctccgaccgc gggcgagaat gccccaccgg tcagctcgga
    27481 gacgcgcagc gcctcggcga cgagagcgcc gagggaggcg gagacgaccg tcgtcgcccc
    27541 ttcggcggct cgcgcgtgca cgcgcccgag ctcggtgccg ggcctccaga gcgaagcgac
    27601 ctcctcgacg tcggagacgg cccgaaacgc ggcctcgaag gctccgccgg tgtcgacggt
    27661 tcccgccggg agccggatct ccaggaccgt ccccatgagc cagcgggccc ggaccttctc
    27721 gaggccgtct tcggcctcgt tcgctcctgc acccaggccc gcggtcgcca ggaagagcgg
    27781 tgcgagaacg gcggccccac gtgaacggcg catcttgaga gacgatctca gcaccgaagg
    27841 gaagataggg ccagcgcgta cccgtgtcaa tcgagaactc gtcgcaatat gatgcaaatc
    27901 atatgatcta acccgtttgc ggttcgctag actccccggc ggaggagagc acatggcgaa
    27961 ctgggagcgc cgggacgtcg tcgtccgcac caccgcgacc ggtcacgagc tgaccgcacc
    28021 ggtgtttacg gctcggggcc gccaggagcg gcccgtggcc tatgtccagg cgaacgtcca
    28081 cggcggcgag ctccagggga acgccgccat cctcggcctt ttcgagctcc tcgagacggc
    28141 gccgcttcgc gggtcgatcg tcctcgtccc gcgggtcaac ccgatttccg caaaccagca
    28201 ggtcggggac tacgtcgcag gggtctacga cctcctcacc ggggtcaact tcaaccgggg
    28261 ctacctctac ctcaccggcc cgtcccggtc ggcgtcggcc gcctgctacg tcgacgtcga
    28321 ttcgttcgcg gccgcgcacc tctcgtcccc catcggagag atccggaccg gtttccggga
    28381 ggcgctcaag gcggccctcg ccgcgattga ggaggaagcg gccgcctggg gagccgactc
    28441 ccggctcgac ttcgcgctcg cgatccagaa gatggcggtc gacgccgacc tcgtcctcga
    28501 cctccacacg ggagaccgcg ccccccgcta cctctactcg ccggaaggag ccgtcgccgc
    28561 cgcgcgtgcc ttcgggctgc cgttcgtcct cgaggtcccg gcccgtttcg gcggcgccct
    28621 ggacgaggcc tctttcgttc cctggcagga cctctccgac gctttccggc ggctcggccg
    28681 ggacgacgtt ccgcgcctcg tcgacggctt caccgtcgag ctcggctcga tgaacgattt
    28741 ctctctggac gccggccggg aggacgcccg gcggatcgca tccgcgctcc gctactacgg
    28801 cgtcctcgag ggcgagccgg acgcccctct cggcccgatc accgcgtgct cggcctccga
    28861 ctaccggtcg atgcacgccc ccgtcggcgg tctcgtcgac ctggccgttc gccccggcac
    28921 gcgcgtgaag gcgggcgacg tggtggttcg gctcgccgac ccgtcgcgct gccggagcct
    28981 gccgccgcgc gccgccgacg ccgtcgtcga ggtccgagct cccgaggacg gcgtcgcgct
    29041 cctgttccac gccttctcgt ccgtgccgaa gggcgcccgg ctcttctcga tgatgacgag
    29101 aacgcgcccc ctctgacgag aaaaggggga gaggatcgct cccctccccc ccgaagcccg
    29161 atcggactcc tctcctccag ccggcctcag ccgtccgact tcaggctgac gtcgtcgatc
    29221 cggaacgtcg tcgtgagaga cgagtcgttc gtggccgcaa agcggacctt cacggtctgt
    29281 cccttgtagg acgtcacgga gtagctcttc agggcatagg tcgacgcgct gcccgtcttg
    29341 ttcaggttcg agagcgtgcc gagcgtggcg agcaccgacc ccgtggacgc gttgaccagc
    29401 tggacgttca gcttgtcgta cgcccgggtg gtcgtcgtct cgctcgtgac gatcgagacg
    29461 tagaacgtga gcgtggcgct cgtcgccccg gacgggatga cgacgttcgc gctcgtcacc
    29521 gactgcgagg aggagttgta gacgccgacg taggcgtagt ccgagccacc gtgcggggcg
    29581 ctgccgttcg cgatcagcgt gttgaagctc gacccgctgt aggccgtccg cgaccaggag
    29641 ccgtccggcg cgctgttcgt cgaggccgtc agcgactcga agctcccgtt cgccaggcgc
    29701 tccacctgtc cgccggagcc gcagctcgcc gtgaagctct tgcccgtggc gttcgggccc
    29761 accgtgacgg agagggacgt cggcgagaag gtgcagccgc tcttcgtggc cgagaccgag
    29821 taggtcccgg cggtcagccc ggtgatcgag tagttgccgc tcgcgtcgga cgtggccgac
    29881 ttcgagcccg cgctgaccgt cgctccgctc gtcccggcgt tcccggcgat ggagtaggtc
    29941 gtcgtgcccg tctgaacggt cgcggtcacc gagaagctcc cggccgcgta gccgtacacg
    30001 ccgagccacc agcgtccggc gctcgggttc gtcgccgtgc aggtctcgtt gcccgaggag
    30061 gtgtacggac ggcaggtata ggtggaggac gtcggcttcg cgttgaagcg cgtgtagatg
    30121 tcgatgtcgg ccgtggcgct cgtcgtctgg aagacgaggc tcgtcgcgcc cgacgggacg
    30181 tcgatgtagt aatacttcca ggcgctcttc gcgacgctct gccccgtgag cgtgacgccg
    30241 ctcgtcagcg ccgtgtcgcc cgtcgagcag gtggccgtga agttcctgcc ggtcgcgctc
    30301 gggccgaccg tgacggagat cgaggtgggc gagaacgtgc agcccgtctt cgacgcggtg
    30361 acggagtagg tgcccgccgc gaggccggcg agggtgtagt tgccgctcgt gtcggaggtc
    30421 gtcgagacgc ccccggcgga gacggtggcg ccgctcgtcc cggcgttgcc ggcgatggag
    30481 taggtcgtcc cgcaggacca ggtgcccggg actccgaccg cgtcccacgc cttgtggacc
    30541 gactgccact ccgccgtgca tgtcccgccg tagaggtccg acgcggcgtt cgccgtcgcc
    30601 gtgcgggcgc cctggaaggt cgtggaagag gtcatgtaga cggagagggc ccggtaccag
    30661 atcctctcgg ccttcgtgag gccgatcccg gtgacggaca ccgtcgtctt gccgcgcggg
    30721 tgagtcccgc cctgggagag gaggtagaag aagaggttcc cgataccgct gttcgtgtgg
    30781 acgccgcagt agtcgttgct gtccgacggc gagcagccgc tcgcgtagtt ccgggtcggg
    30841 tagtagtcgg ccgagtcgcc gtccttggcc gggtcgtaca tgtagcggag ggcgtcgccc
    30901 gaggtgcccg gggtgtagac gtcggcgccg accgcccagt gggtccgcgt cgaggcgttg
    30961 ccgtccccgc cgtagaacgc cgcggcgcgg ccgaggatgt cggagttcgc ctcgttcagg
    31021 gcgccggagt cgttctggta ggcgagatcc gcggtccgcg agcagacggc gtgcgagaac
    31081 tcgtgcgtcg tgacgtcgat gccgcgcgcc aggttcgaga acgtgctccc gtcgccgtcg
    31141 ccgaagacca tctggttgta gggcgaatcg atccaggccg cgttgttctt cgagcagctg
    31201 tagcccgtcg agaactggcc gtggaccgag gagacgatga ccgagccgga gccgtcgtac
    31261 gagtcccgcc cgtggatgtt cttgtagtag tcgtaggcga ggcccgtgtt gtcgtaggcg
    31321 cccatgaccg ccgtgtcgga cgacgtgccg ccctcgttga tgacgagcgt cccggggagc
    31381 tcgtttccgg tcgagaggca ggcctggttc atgttgtaga tcttccggtt cttcgccgtg
    31441 aagatcttcg agagggaccc gacgaaggcg ccggtcgccg cgtccacgtg gacgtcctcg
    31501 aaccggagct cgtcgtcctc gacccacatc gcgcggatcg ggacgacgag ggccggctcg
    31561 cccgaaagcg cccagacgac cgggtcgagg acgtcgacga tctcgacgtt cgccccgccc
    31621 tcgcgttcga tccaggcgat cgcggctccc gccgactcgg ccggggcgag cgcggcgacg
    31681 accggaaggt ctaggccgcc gacgaaacgg ccgctcatcc cgaggagccc gtccccgtcg
    31741 aggtggacga cgacgtcgcc cccgaggact cggatgcccc gataggtctg catcatcctc
    31801 acgtgggact ggccgagctg gtccttctcc tccagccgga acgcgaagcc gtcctccggc
    31861 ccgcggccga ggacgtcgcg gtactcctcg agcgcctcgg cggccgcggc gagcggctcg
    31921 cggcgcacga cggcgcccgc ccgcatcgct tcgccgacgt gcggctcgcg gaccctgagg
    31981 tcgacgtcgc gggccgccgg acgggcgatc ccctcgtccc cggcgggacg agcgagagag
    32041 tcgcccggca ccagaagagc cgccgcggcg aacacggacg ccaggaacag gccgtactta
    32101 cgattcatgc agtccctcca gcccggatac ggtcgaagac cgtctcgatg agattcgggt
    32161 cgtgcttcgc aacggtcgtg ccgtggtccg aggccggggc gcagccggcg tcccggctgc
    32221 ctgacgacga gcgaacctgc attccgtgcg gcccgcgcgc cgcccggtct cgctgagagc
    32281 gtgggggcaa cggcatcgaa agggcgcgca tggggtcgat ccctgacccg ataccagggt
    32341 cgaactcgtt caccgggtca ggttctggac cgcgggctca gcgaaaggag cctcgtcgcg
    32401 ccccgccggg ttctggccga taatcccccg ctcaccaagt gaccggaggg aacgtggcca
    32461 cgatcgagtt cacggggaac tacgaggacc tctcgaccga caagggctat cagttcaagt
    32521 tcttctgcga gaagtgcggg aacggctaca tgtcgagctt caaggcgtcg aagctcggga
    32581 tggcgacctc ggcgctccag gcggccggca acatcttcgg cggcctcctc gggcgggccg
    32641 cccacggcgc ctacgaggtg cagcgggccg tcggcggccc ggcgcacgac gccgccctga
    32701 aggacgccgt cgccgagatc cgcccgctgt tcatccactg cacccgctgc gggcagtggg
    32761 tctgcggccc cgcctgcttc aacaagaagg ccggcctctg cgagggctgc gcgccggacc
    32821 tcgacgagga gatggcggcc gcccaggcgg aggccgcgcg cgagcaggtc cagcagaagg
    32881 cccgcgaggt cgactggatg aagcagcgcg acgtcgcgac ggtgacgggc gccgtctgcc
    32941 cccactgcgg ggcgaagacg cagggaggga agttctgcgg cgactgcggg aagcccctcg
    33001 cgacgaagaa gaagtgcgtc acctgcggca ccgaggcgga cgggagcccg aagttctgcc
    33061 ccgagtgcgg cggccgcttc tcctaacggg ggccgcgacg tgagcggcgt cgaggagacg
    33121 cggcgcgccc tctgcgcgtt cgagaccgag gggatcgcgg acgcgcccgc gcacggcgag
    33181 ggcgaagccc ggatcgacga cgagggcgtc tcggtcggcg gcttccgggt cgagttcctc
    33241 gacgtggacg tccttacgga cgagcggcgc gtcctgacgc tcggcctcca tcccgcggga
    33301 gccctgcgga tctcgatgct cgcccggcgt cacgagacgt tcgcgaaggc gctcggagag
    33361 gcccgcgatt ccgcgcgcat tcggggcctc ctcgcccacg gcctcggcgt tcccgaggcg
    33421 ttcgacggcg cgctcctgga gccgggcccc gcgcgcgagg cccggctcct cgtctggccg
    33481 acgcacgtcg ccgtggtccc cgccggcgcc gactcgttcc aggtgccgct cggcggcgtg
    33541 gcggacgtga ggttcaccga agggacctgg gaggtcgtcc tcgacgctcc gggcggcccc
    33601 tttcacttcg gtcgcctcgc gcgccggacc gacgcgttcg cgcgctcggt ccgcgccgcc
    33661 cgcgcggcga tgctggagcg ttgcgcgaac gcctccggca cccggctctt cgccgatggg
    33721 cgcgccgttt ccgcccccgc cctcgcgagc gacttcgagc ggctcctcga ggcctggagc
    33781 gccgccgagc ggctcgacgg cgcccgggag atcgtccgtc gcgccgggcg cgaagcggcg
    33841 tcgatcggcc tcgccgagct gctcgacccg gacgagggga gtctcgcggc gaaggcggag
    33901 ctcccggtga acacggcggc cttcctcctc gccgaggtcc ggggccgcgt cgtcctcgag
    33961 ctcctctcgg gaccgtccgc cgcgacctac gtcttccggg ggacgcgcgc ggagattggg
    34021 gccgacctcg cggcacttca ctttcgacgg cgcggcctcg ccctcacgcg ggccgaggag
    34081 agcggccccg cgggccgttc ctatcggctc gcgctccggc gcctcgaccc tctcaagcgg
    34141 cttcgcaccg ccaccctcgc gcgcgtcgtc cacgacgagc gctggtcgca gcgcctcgcg
    34201 aaggctctcg acgcgacctg acgagcgccg accggcacgg cggcgccccc tcccgtccgc
    34261 gccccgatcg gacggcgacc gcaccggctc ctccgctttt cgcttgacaa cgccccggtc
    34321 ccttcccgta ccctgccgcg ccatggaacg ctccgccgcc ccgcgctcgc tcgcgagcac
    34381 gctcgtcgcg ggtggtgcgt gcctgccggc ctgcgtcctc gccatgtcca tgtcgatgcc
    34441 catgtgcggc tcttccctgc tctcgcagga ggagtcgggg tacgcggcgg gacggcgaat
    34501 gtgacgcccg cagcgtaacg agccgaacga cggcaccccg accggccctc tcgcgagaga
    34561 gggccggttt ttcttttgcc cgcccgccga gaacgaaagg agcaccgatg tccgcgaccg
    34621 ccaccgtccg ccccgccctc cgccccggca ccctcgcgat ccacgcgggc caggagagcg
    34681 ccgaccccgc gacgaatgcc cgcgccgtcc cgatctacgc gacgacgtcg tacgtcttcg
    34741 acgatcccga ccacgcggcc gacctcttcg gcctcaggaa gcccgggaac atctacaccc
    34801 ggatcatgaa cccgacgacc gacgtcttcg agaagcgggt cgccgcgctc gaggggggcg
    34861 tcgccgccgt cgcgacctcg agcggacagg ccgccgagac gctcgcgatc ctgaacctcg
    34921 cccgcgccgg cgacacgatc gtctcgacga cgtcgctcta cggcggcacc tgggccctct
    34981 tcgcccacac gctcccccgc ctcggcgtca cgacccgctt cgtcgacgcc tcgggcccgg
    35041 gcggccccgc gcgtgtccgc gaggcgatcg acgggacgac gaaggccgtc tacgtcgaga
    35101 cgatcggcaa cccgcgcctc gacgtgcccg acctcaacgc cctcgccgac gccgcccacg
    35161 cggcgggagt cccgctcgtc gtcgacaaca cgttcgcgac gcccctcctc tgccgccccc
    35221 tcgagcacgg cgccgacgtc gtgctccact cggccacgaa gtggatcggc gggcacggga
    35281 cggcgattgg cgggatcgtc gtcgacggcg gccgcttcga ctggggcgcc tccgagcgct
    35341 tccgcccctt cttcgtcgac ccggagccgg cctaccacgg cctctccttc tcggccctcg
    35401 ccccggcggc ctacgcggcg cgcctccgca ccgtcctcct ccgcgacgtc ggcgccgccg
    35461 tctccccctt caactcgttc ctcttcctcc aggggctcga gacgctcccg ctccggatcc
    35521 ggcgccacgc ggacaacgcc ctcgccgtcg cgcggcacct cgagagccac cccgccgtcg
    35581 cctgggtccg ttaccccggc ctcccctcgc acccgaccca cgagacggcg gcccgcgtcc
    35641 tctccggcgg cttcggggga gtgctcacct tcggcgtgag gccccccgcg ggcgtctccg
    35701 ccgagcaggc ggcgcgctcc ttcatcgcga acctctcgct cttctccctc gtcgcgaacg
    35761 tcggcgacgc gaagagcctc gtcatccacc cctggacgac gacgcacgaa cagctctccg
    35821 aggaggagcg gctcgccgcg ggggtctcgc ccgacctcgt ccgcctctcc gtcggcatcg
    35881 aggagcccga ggacctcctc gcggacctcg acgcggccct ctcccgcctg ggcgcgccgg
    35941 gacgaacgcg atgagtgccc gcgtcgagac cctcgtcgtg aagttcggcg ggacgtcgct
    36001 cgggacgccc gcccggatcc gccgtgcggc gcgccgcgtc gcggccctcg tcgcctccgg
    36061 ccggcgcgtc gtcgcggtcg tcagcgcgat gggccacacg accgaccaga tcctcgagac
    36121 cctcggcgtc ctcgaagccg gacgttcggc ctcggcggcg cgggaggtcg accgcgccct
    36181 cgcgaccggc gaggacctct ccgccgccct cctcgccgcg gcgcttcaca cgctcggtgt
    36241 cccggcgcgt agccttcgcg gcggcgaggc gggcgtaaga gccgacggcg ccttcggcgc
    36301 ggcccgcgtc gcgcgcgtcg acgcccggcg cctccgcgcc ctccttgccc gcggcgtcgt
    36361 ccccgtcgtc agcgggttcc agggggagcg ctccgaccgc gaggtcgcga cgctcggccg
    36421 gggcggctcc gacacgagcg ccgtcgccat cgcggcggcg ctcggcccgg cgccctgcca
    36481 catcgtcacg gacgtcgacg cggtctacga ccgcgacccg cgccgccacg ccgacgcgcg
    36541 cccgctcccg cgcctctcgc acgacgagct cctcgacctc gcacgctcgg gcgcgcaagt
    36601 cgtcgccgcc gacgcggcgc ggctcgcggc cgaacacggc gtgccgctcc gcgtcctcgg
    36661 atacgacgcc gcgccgagcg acgagcgctc gggcaccttc gtcgggggcg cgcggtgaac
    36721 ggcccgcccg acgccgcggc cctctcggcc ggcctctcgg agcaccgggc gggcgacttc
    36781 acgctcgagt cgggcgagac gctccgcgac gtgcggcagg cgttccgcct cgagggggag
    36841 gtcgacgccg aagggtcgaa cgtcgtcctc ctccttcact cgctcaccgg ctccccggcc
    36901 gacctcggcg gctggcgccc gttcgtcgga ccgggcctgc cgatcgacac gcgccgcggc
    36961 gccgtcctcg ccccgaacct tctcggctcc tgctacggca cgcgcttcca ccgcggcacg
    37021 gagcacggga ggacgcccct ctcaatcacg acgcgtgacc aggcgcgcct cgcctctctc
    37081 ctcctcgacg ccctcggcgt gcagaggctc gcgctcgtcg ccggtggctc cctcggcggg
    37141 atggtgacgc tcgagctctg cgcgacgcgc cccgacgcgg cccgcgcggc gatcgtcttc
    37201 gcggcccccg cctcgccgac ggcgtggggc tcggcgtgga accacgtcca ccgaacggcg
    37261 atcgaggcgg cgccgcgctc cggcctcgcc ctggcccgaa tggtcgggat gctgacctac
    37321 cggacgcctc tcgagctgga gcagcgattc cgcccggacg gagccgggcc ctgccccgtc
    37381 cgaggctacc tcgaccacca cggggagaag ctcgcccgcc gcttcacggc ccgctcctac
    37441 cggacgctcc tcgacgcgat cgactcgcac gacgtcgggc gcggacgcgg cagcgtgggg
    37501 gccgcgctcc gccctttccg cgggcgcctg gtcggcgtcg ggattcccgg ggacctgctc
    37561 tacccgcccg aggaggtcct ccgctggacg cgcgccgcgg gagccgagta ccgcgagctc
    37621 gcctcgcaga gcgggcacga cggcttcctc ctcgagacgg gagcggtcgg cgcgctcctc
    37681 gtggaggcgc tccccctcgg caaggcggac cgggcgctcg cgttcggccc tctcgccgct
    37741 gctaatctcg gcgccggacc tccgccgtga tcgccgtgcg tcgcctcgtc acgagccctt
    37801 tcgcgtcgct cgtcctcgcg gccggcctcg ccgccggctg cgggaagggg cggttcgagc
    37861 cggctccggc cgcgctcccc gagggaaagg tatgggtcga gcacctctcg cgcgacctcg
    37921 ccccgttctg gctgacgccg caggccctcg gctcgccggt tggcgccttc cccacgttcc
    37981 gctgcgacga cgggagcctc gtcgaccccg agctcccctg cggcgagatg ctcgtcgcgg
    38041 gcccctgggt ctcctcgcgc ctcggagtcg agtacacgcg gatgcgctcc cgccagacgt
    38101 tcttctacgg cgtcgccttc cacctcacgg gcgaggagcg ctacctcgcg ctcgcgaagg
    38161 cgggcgtcga ccacctccgc gcgcacgcgc tcgaccgggc ggagcggagc gccgtgtcgt
    38221 ggtggaagga cggcattgcc cggccgcccg tcccggcgcg cagctcgcaa gacctcgcct
    38281 acgcgaacct gggcctcgcg ttctgggcct acctgacgcg cgacccggac gtcctcgcgg
    38341 acgcgctcct cctctacgag gggaccttcg accgctacga cgacccggag cgcggcctcc
    38401 ttcggtgggc ggccgggggc gtcggcggcg agacggcgcg cgtcgagctc gtcgcccagc
    38461 tcgaccaggt gaacgcctac gccctcctcc tcgcctcgtt cgcccccgag ccgcaccgct
    38521 cgcgcctgcg tgccgacctc gcgcggatcg ccagggtcct gatcgaccgc ttctggagcg
    38581 aggagctcgg cctcttccac ggctccctcc acgatcccgt ggagcgggga cccggcgccc
    38641 gccacaccga tttcggccac acggtcaagg cgctctggat gatcgagagg accggcgcgc
    38701 tcctcggcga tgccgccctc tccgcgtggg cgcgcgagcg ccttcccgcc ctcctcgaga
    38761 gggcctttct cccgcggcac ggctgctggg ccaccggcct ccggcccgac ggctctctcg
    38821 acgccggcgt cacctggtgg accgccgccg agctcgacca ggccgccgcc acgctcgcgc
    38881 tcacggaccg ccgatacacc ggctacctcg agaggacctg gacctgctgg ctcgaccggc
    38941 tcgtcgacca cgaggacggc gaggtctgga gctggatcga cctggaccgt cccgagcggc
    39001 gcggcgccaa gcagcacctc tggaagaacg gctaccactc ggccgagcac gcgctcgtct
    39061 cgtatctcgt cgcccaggag ctgcacggcc ggcccgcgac gcttcacttc gcgcttccgg
    39121 acggggcgct tcccgatggg ctccgtccct atttcttcga cgatgcggcg gaggtcacgg
    39181 ccgtgacgcc gctgcgcggc gcgccgggcc tgcgcaatgt cgccgtctcc ttccgcggga
    39241 tccgctgagg ccgctgcgac cgccggccgg cctacactct ccggggagag aaggcgcgtg
    39301 agaaccgaga ggaccggccc cgcggcgttc acggtcgagt tcgagtccga ggccgagctc
    39361 gaggccgagg cgcgcacgaa cctctccgcg ggcgggctga ggctcccgac gcgcgagaag
    39421 ctcgcgatct tcgcgaagct cgagcttctc gtgaggctcg cggggggcgg cgcggtcccc
    39481 gcgacggcga cggtcgtcgc acccctcccc gacggcttcg cccttcacgt ggacggagac
    39541 gccaccgccc tcgccgaagc gctccgcgac gctcccgaag agcccggaga ggaggtcctc
    39601 gaggacgtcg cggaggcgga ggagaccgcc gtccgcgaga gcgtctggga ccgccttcgc
    39661 ggcctgacgc gcgtcgagaa gctcctcctc gcggcgaagg cggaccgcgt cgagcggatg
    39721 atcctcgccc aggacagcga cccgcaggtc ctctactccc tcctgaagaa cccgcgcctc
    39781 accgcagacg aggtgatccg gatcgcgaag tcgccgttcc tgacgttcca gaccgccgag
    39841 gcgatgatga agtcgaccca gtggttctcg aacctcgagg tgcgcgtcgc gctcgtccac
    39901 aacgcgaaga cgccccccgc gttcgccctc cggatcctcc cgacgctccc cgagtcggag
    39961 gtgaggaaca tctcgcgcgg cgccgcgacg agcatggcgt tgaaggtcgc cgcgctgaag
    40021 aggctccagg gcttcacctg agccgtcccg gccgcgggtc catactcacg agggccggca
    40081 ggccgtcgcc tcccggctcg aggagggagc gatgcccgag cccgcgatcc cgtccctggc
    40141 cgaagcggcc gccgcgctcg gccttccgcc ggaacgcctc gtcccgtacg gccacgacaa
    40201 ggcgaaggtg ctcccgtcgc cggccgggtt cacgcagcgc ggccggctcg tcctcgtctc
    40261 ggcgatcacg ccgaccggcg ccggcgaggg gaagacgacg acctccatcg gcctcgccca
    40321 gggcctcgcc cggatcggcg cgcgcgccag cgtcgcgctc cgcgagccct cgctcgggcc
    40381 gacgttcggg atgaaggggg gcgcgacggg cggaggccgg gcacgcctcg tcccctcgga
    40441 cgacatcaac ctccacttca cgggcgactt ccacgccgtc acctcggctc acaacctcct
    40501 cgcggcgctc ctcgacaacc acgtccacca cgggaaccgg ctcgagatcg acccgaggcg
    40561 ggtcttctgg cgccgcgtcc tggacgtgaa cgaccgggcg ctccgccacg tcgtcatcgg
    40621 cctcggaggc gtcctccagg gggtgccgcg cgagaccggc ttcgacatca ctccggcctc
    40681 cgaggtcatg gccgcgctct gcctcgccga ggacgcggag gacctgcgcg cgcgcctctc
    40741 gcgcatcgtc gtcgcgctga cgttcgggaa ggagccggtc acggcggggg acctgcgagg
    40801 ggcggggtcg atgctcgcgc tcctgcgcga cgcgctcctt ccgaacctcg tccggacggg
    40861 cgagggggtg ccggcgttcg tccacggcgg cccgttcgcg aacatcgccc acggctgcag
    40921 ctcggtgatc gcgacgaaga cggcgctctc gctcgccgac tgggtcgtca ccgaggccgg
    40981 cttcggcttc gacctcggcg cggagaagtt cttcgacatc aagtgcgccg gggcgggcct
    41041 cgacacggcc gccgtcgtcc tcgtggcgac cgttcgggcc ctgaagcggc acggcggcgt
    41101 ttcgaaggag gggctcacga cgcccgatcc ggccgccgtc gagcggggcc tgccgaacct
    41161 cgcgaagcac ctcgagagca tccgcgcgtt cggggaggtg ccggtggtcg cgctcaaccg
    41221 gttcgcgacc gactccgggg aggaggtcgc cgtcgtccgc cgcgccgcgg agggctgggg
    41281 cgcgccgttc gccgtctgcg acgcgttcga gaggggaggc gagggagcgg tcgcgctcgc
    41341 cgaggcggtc ctcgcccacg cggagcggaa gtcgagcccg ttccggccgc tctacgcgcg
    41401 cgaggacccg ccgaaggaga agctggcgaa gatcgcccgt cacatgtacg gcgcgtcgtc
    41461 cgtcgagtgg agccgggagg cggagcgcga cctcgcggag atcgcccgcc tcgggtacga
    41521 gaatctgccg ctctgcgtcg cgaagaccca gatgtcgctc tcggacgacc ccggggtcgt
    41581 cggccgtccc gaagggttcg acgtcaccgt ccggggcgtc ctcctcgcgg cgggcgccgg
    41641 ctatctcgtg ccgctcctcg gcgacatcct gcggatgccg gggctcccct ctgttcctca
    41701 ggcggagcgg atcgacctcg tcgatgggaa gatcgtcggg atcagctgac gaccgggccg
    41761 cccgcccgtt cgttcacgaa cgcgacgagc tccttcaggg ggaggggctt cgagaagagg
    41821 tacccctgga agtggcggac gcctcgttcg acgaggtact ccacctgctc ctcccgctcg
    41881 acaccctccg ccacgaccgt catctcgagc ttccgtccca gctcgacgag agtgtcgacg
    41941 accgtctcgg cgaccgagcc ggtgccgatc ccctcgacga agctcttgtc gatcttcagg
    42001 tggtcgagac ggaagcgccg cagcgtggcg atgttcgagt agccggtgcc gaagtcgtcg
    42061 agagcgatcg tggcgcccca cccctggagc gcctcgagga cgtttcgggc cgtcccgtcc
    42121 acgtcgtcga tcacctcccg ctccgtcacc tcgaagacga ggcccttcgg cccgagccgc
    42181 gagcccgcga acgcgcgcag gacggccgcc acggtcccgg gatcgcgcag gtgcgccccg
    42241 gcgaggttga tcgccacgta gaagcggtcg ggctcgcgga gaccgccggg aaggtcccgc
    42301 gcgacccgtc gcatgagcca ctccgtgatc gggacgatca gctccgtctc ctcggcgagc
    42361 gcgatgaaga ggtcgggcga aaggagcccc tcccgcgcgt gacgccaccg gaggagggcc
    42421 tcggcgccga cacagcgacc ggacgacacg tccacgatcg gcaggtacct cacctccagc
    42481 tcgccgcgcc ggagcgctcc gcgcagctgg cgcgtgagcg ccgcgccggg cgatcccgcg
    42541 aggaggacga gcgcggcggc gccggcgccg aagccgaggc cgatcgcgac gagaagcggg
    42601 agggtctctc cccacgcggc gagctgcgcc tcccggcggg cgtacaggtg gacggagacg
    42661 cccccgccgg cgcagggggc gacagcggtc tcgtcccgcg cgccgacgag cgaaccgagg
    42721 aggaggagga acggcgaagc gaggcgccgt gaggcgggag ccgcggggat ggagaggacg
    42781 ggaccggcga cgccccggac ttcgacggcg tcccggccgc cccccgcgag ctccgtgaga
    42841 gcgcgcgcga tcggaccggc gtcgagggcc gcctcggccg aaccctccgg gagccgcagc
    42901 gcgaggcgga tgaggccgcc cggcgccccg gcctcccaga cggcacgcgc gtcgcggccg
    42961 agcgctcccc ggacggacgg cgacgggggc accggcgccg tcccggccac gcagacgggg
    43021 cggagctccg cgtcgagcag cgcgacgcct cggacgccgg ctccgacctc gagcgcggaa
    43081 cggagcgatt ccgcggtctc ggccccgcag cctccgtccc agtccgttcc gagccgtgaa
    43141 aggaggccct ccgtgctcga gaggagggcg tcggtgagcg cggcggccga ccgggcgcgg
    43201 cgctcgaggt cgtcgcgcag ggcccgccgc tcgaggcgga aggagagcca gacgaggacg
    43261 gccggcggca gcagcgccgc cagaaggacc gcggcgacga gacgaacggg ccagcgcgtc
    43321 acggcgctcc ctcgtaggcc cgcgatcgac cgctcttcat cggttcgccg attctacgga
    43381 gcgtccctcc ccgctcaacg gaggtaaccg agcgagcgga gactctcgac gtactccttc
    43441 gggtcgatcg gcgccgcgac cccgcggggc cgcgcgcccc acgagccgac ggtcgcggtc
    43501 gtggcggaac ccgcccggac gatccgggag tcgacgcgcc cttccgcctg acgcgaggcg
    43561 gggacgccga ggacggcgag gagcgtcggc gcgaggtcga tcggccggac ctctccggac
    43621 gcgccttccg ctgcgagagg cccggcgacg agcagctccc cctcccggcc cgtccgcccg
    43681 gggtcgagga ccacgacgac gagctccgcg gaggagccga ggacatcggg gccggcgagg
    43741 aaggaggcga gcgatcgcgt ctcctcggcg agggcggccg cgagcgtggc ccgctcgccg
    43801 acggaacgtc ccggctcgga gagcgccgcc ccgaggatgt cgaggcccgg aagatagagg
    43861 agggaaaggc gcggccgcga tcgggccgtc tctccgcgga acgcggcgat cgcgaacgca
    43921 tcgagccccc gcgcgtcgtc gaccaggcgc tgggcaccgt cggccgccgg ggccgccggc
    43981 ggcgccgcag tcgcggcgcg cctcaccgcc tccgccggcc agccctcgcc ctcgagcgcc
    44041 ccccccgcac gcgcggcgaa gtagaggtgg ttcccgagga cgacccctcc cgcgtcgccc
    44101 gcgggccacg tcgtccacca gttgaccgtc agcgacgcga ggccgcctcg atgggccacc
    44161 tcccagatcg ccgggaccct ccgcgcggcc gacgtgaccg actcctcgcg cgccacgccg
    44221 agcgccggaa ggagccgccc gaggtaccag ccggtcccgg cggtcagctt caccggcgcg
    44281 gagacaccgt cgagacggac gagatcgagc gcccccacgc cgtgccgtcg cgcgacctct
    44341 ccagtcgcga ccgtcgtcca gaacgccgcc gggtccgacg tctccttgcg gtaggagtag
    44401 cgtgacgcgg cggggaccgg caacgcgccg cgcgcaagct ccgggaccca gccgtccacc
    44461 gccaccagca cgacccgggc cgggcccgca cggacgacga gcggctcggc ccgcggctcc
    44521 ggacgcctcg cgaggagcca tccgcccgtg gccaccgcga cggcggcgag cgccgtcccg
    44581 agcgcgagtg cgggccgcgg cgccctcgag aggaccgagc gacccgacgc ggcgaggacg
    44641 gtgaacgcga gcccgcgtgc gacgcggacc gcgaggacga cggcggcggc cgcggcggcg
    44701 aacgccacgg ccgcggcgac gggtcccgcg gcgtcgagga cggggcgcgc ggcgagggcg
    44761 gcgcccacgc ccgcgagaag gccgaagccg acggcgacct ccgtcgaggc gcgggacggg
    44821 tcgctccgtc cgcgcgtccg cagccaggcg atcgccgcca gggcgaggac gagcgcggcc
    44881 gcggcgacga gggccccggc gcccagccag gcgaagagaa cgagggcgtc gcgcggggag
    44941 agggcggagg agagggcgac ggcggccgtc tcgacgacgg cgagggcgag ggcgaggagc
    45001 agggaggcca gcgcggctcc gcgcaggagc gcccgcgtcc gcgtccgcgc ggcgagcgcc
    45061 cccttcagga ccgcgccttc gaccccgcgg tccaggtagc cgcgcgcgcg aagacgctcg
    45121 cgcgcctcct cgaacgtcgc gggatcgcgt tccgaagggg tcccggcggc gggttcggtc
    45181 acggctcgga ccttagcagg gcgcgggacg cggtgcccgc cttcaggccg tccgcgcgcc
    45241 gaggagcgac gcgaactccc ggaggagcgc ccccttcgcg ccgaacggct cgagcgcctc
    45301 cacggcgtgc gccgtcagct ccgcgacgag ccgccgggcc ccctcctcgc cgacgtgccg
    45361 ggcgaaggtc ggtgccgggg cgcgattcgc gtgcttcccg gtccgggccg ggtcctgccg
    45421 ctccagcagg tcgtcgacga tctggaacgc gaggccgacg ttccgcgcgt aggagcggac
    45481 cgcggcgagc tcgcgctccc gggccccgcc caggaccgcc ccgaactccg cggacgccac
    45541 gaagagggcc ccggtcttcc gggcgtgaat cgtctcgagc cggtcgaagg aggccgccgc
    45601 ctccggcgtc cggaggtcga gcgactgtcc tgaggcgagc ccgaggagtc ccgtcgcgtc
    45661 ggccaggcgg gagacgaggt cgagcgccgt ggccggccgg agcgcggaga gcccggcgtc
    45721 ggcgaggacg ccgaacgccc ggacgatgag cgtgaccgcg gcgaggatcg cgaggtcctc
    45781 gccgtggacg gcgtggagcg ccgggcgtcc ccgccgcgtc gtggcgtcgt ccatcgacgg
    45841 gaggtcgtcc agcgcgagcg acgcggcgtg gatcatctcc acggcgcagg cggggtcgag
    45901 gagacgctcc tccggcgcgc cgaagagctc gccgacgagg aagacgaggc ccgggcggac
    45961 cctcttcccg ggcgagagga gcgcctcccg ggccgccgtg gggagcgcgt ctccgacggc
    46021 gggggcgccg gctcccgaag gctgagcgga gaggagcgcg gggagccgat cgtccaccgc
    46081 gcggcggacg cgggcgaggg tttccgggag ggagatggac acggcgggcg actccggctg
    46141 cgatcggcgc gggcgattcg gaggagcgga aagtgtagca tcggcgcgat gccagccgcg
    46201 tcgacgaagc cctcgtcccg cgcgcgggaa tggctgtcgg ccctcctggc gccggcggtc
    46261 gtcctgctcg ggttcgccgc tctcgctctc acccgcggtc catcgcccct tcgggtcctc
    46321 acgaccggcc tcctgacggc cgcggcggtc gcggcgctcg gtcggctctc ggccggccgc
    46381 ctcctgcgct cgcgggaagc cacgcgcgac gcgctgcacc gagcctcggc cggcgacctc
    46441 acgctttcgc gggccgcgct cgcgcggtcg ggagagaacg cgctcgccga ggcgctccac
    46501 ggcctgatgg tcgcgatgga gcggattctg gcgtcgttca cgcgcctctc cgcagccgtt
    46561 tccggcgtgg ccgtggacct cacgacgcgg ggacgggagc tctccctcgc cgcggcgatg
    46621 cagagctccc gcgcggagga gacggccgac gcgatgcgcg gcaccgacgc ggcgatcggc
    46681 tcgctgcgcg agtcgatgga gacgctcgcg ggagccgccg agaacgccgg cgcctcgctc
    46741 cacgagatga cggcctccgt caccgaggtc tcgcgaagca ccacggcgct ccgggcgttc
    46801 gtcgacgaga cgaccgcctc gctcggcggc gtcgtctccg ctcttcagca ggtcgcctcc
    46861 gccgtcgaga acctctcggg cctcgcggac gaaacggccc gcgccaccgc cgcgatccgc
    46921 tcctccaccg ccgagaccga ccggcaggcc cagaccggcg cgcggctctc cgagcgcgtc
    46981 tcggcggccg tcgtctcggg gaagaccgcc gtctcgggga ctctggcggg gatgcacgag
    47041 atccgcgccg ccgtctcggg cgcctccgac gcctccacgg cgctcgccga gcacagcgcg
    47101 cgcatcgggg agatcctcca cgcgatcgag gagatcgcgg gagagacgaa cctcctcgcc
    47161 ctcaacgcct cgatcatcgc cgcccaggca ggcgagagcg gcaagacctt ctccgtcctc
    47221 gccgacgaca tccgcgacct ctccgagcgg acggccgtct ccaccgagga ggtccgcggc
    47281 ctcgtctcct ccgtccgcgg gggcgtcgag gacgtccgca ggctcctcgc cgacgcgcgg
    47341 cgccgcacgg aggagggcgt cgacctcgcc cagacctccg acgccacgct cgacgagatc
    47401 gaggcgctcg cgctcgagtc ccgccgcgcg tccgagggga tcgccggcat ggccgtggcg
    47461 cagagcgccg acgccgcccg cgtctccgag gcgaccggcc gcgtctcggc cgaggtcggt
    47521 cgaatctccg aggcgacgcg cggccagctc gagacgacgc ggaccgtcca cgcgcgggcg
    47581 gatcgcgttc gagagctcac cgagcagctg gcccgggcga tggaggagca ggccgtcgga
    47641 ggccagacgc tcctcggctc gatggagcgc gtcacctcga cggtcgacgc catcgcggag
    47701 gcgaccgtga cgctcgccga cggctccgcc gccgtggtcg ggtcgatgga cgagattcgc
    47761 agagccgccc ggcagaacgc ctacgccgcg acctcgatga accaggcggc ccaggcgctc
    47821 gagcaggaat ccctactgct ccgttcccgc tcctcgctgt tccgccttcc ctcgcccgcg
    47881 cccggcgggc ggattcgcgc cgcgctgcgc tacctcgacg aggcgaactt cgaccccgcc
    47941 ttcagccaga ccgtgcccca ggccatcctc gcgcggacct ggggagaggg gctcgtcaag
    48001 ttcggcgagg ggacccggat cgtccccgag ctcgccgagc ggtgggagat cgacccgacc
    48061 ggaaccctct tcacgttcca cctccggcgc ggcgtgaggt ggcacgaggg cgggaccctc
    48121 tcggcggccg acgtcaaggc ctccttcgag cgcttcctct caccgtccac cggcgcgccg
    48181 ctcgccgccc tgttcgacgc cgtcgaggga tacgaggact tccgggccgg acggagcccc
    48241 ggcgtcgccg ggctcgaggc gccttccgga tcggtcctcg ccgtccggct ctcgcggccg
    48301 gtccccttct tccttcagct cctgacgctc cccgacatca cggtcctccc ccccgcgatc
    48361 ctgggcgacc cggagaggat ccgtcgcttc ccgtgcggca ccggcgcgtt cgtcccgcgg
    48421 gagatcgcgt tcgggaagtc cgcgcgcttc gaccgcttcg acggctactg ggagcggaac
    48481 cggatcgcgc ccgacgggat cgacctcgcc ctggccgagg actcggaggc cggcgtcttc
    48541 cagcgcttcc tcgacggcca gctcgacgtc gtctgggaca tcccgtaccc cgaggcggcc
    48601 cgcctgatgg gcgacccggc ctggcggccc tacatcgact cctcggtcca gctccacacg
    48661 tcgttcctcg tcctccggtg cgaccgtgcg ccgctcgacg acgtccgggt ccgccgggcg
    48721 ctcaactacg cggtcgacag ggcggcgctg aacgagagga ccttcgcggg cctctccatc
    48781 ccggcggcct cgatcctccc gcctcacgtc gtcgggcacg accccgcgct ccgcccgttc
    48841 cgtcacgagc cggagaccgc gagggcgctc ctccgcgagg cgggccacgg cgacgggctg
    48901 acgctctcgg cgtggctcag cccgaaggac gcgcgcgacc cgctgaatcc gctcccggcg
    48961 atcgcgaccc agctcgaggc ggtcggcgtg aacctcgcgc tcgaggtgct tcccggcgag
    49021 gaggcggcgg cccgcaagag gaacggcgtc ttccccgacg tgaggctctc gcgctggttc
    49081 gccgacttcc ccgaccccga cacgttcttc cgctcgctct tctcttcgaa ggccgagagc
    49141 gtcctcgacg gcggctacgc gaatccggag gtcgaccggc tcgtcgaaaa gggcgcccgt
    49201 ctgctcgacg cggccgagcg cgaggcgctc taccgcgagc tcgaccggct cgtccagtcc
    49261 gaggccccgc tcgtcttcct cttccacaac cgcggcttcg tcgcgcacgc cccgcgcctg
    49321 cgcggagtcc aggcgcacct cctcctgccg ccggtgaggt ggaacgagct ctggctcgag
    49381 ccctgaacgg gacgccgcgg gagacgcaga cgaaacggcc cggcgcgctc cgttggaggc
    49441 gggccgggcc ggagggtcgg cggcgctgag gggggtcagc cgttgaagct ggcgatcgcc
    49501 tcggcctcgt tggggtagac gtcgaagacg gtgatcagct gcgtcacggt gaggacgtcg
    49561 ctgatcttgg ccggcaggtt catcagcttc atcttcgcgc ccttgtgcgt ggcggtcgtg
    49621 aagcagccga cgagctcgcc gacgccggag gagtcgatcg tgacgacctg cgacatgtcg
    49681 acgacgagct tgcgcgcccc ttccgcgaga gcggcgtgga tcgcctccct catctggagg
    49741 tcgccggcgc cgatcgtgat cttccccttc agctcgatga tcgtgatttc tcccgccttg
    49801 cggatgctgg cgttcttgtt cattgcttct ctcccttcgc gtttccgcgc ttgctcaggt
    49861 ccttgcgcat cgtcaggacg gtgccgcccc cctcgccccg cgagaaggtg acgtcgtcca
    49921 tgaaggtccg gatgtagaag atcccgcggc ccgaggtctt gagctggttc tccggggcga
    49981 gcgggtcgga gacccgttcc gggtcgaagc caccgccctc gtccccgatc acgatcgtca
    50041 gctcgtcgcc cgccgactcg agcctcacga ggacgcgctt gtcggggtcg agccggttgc
    50101 cgtgcttcac tgcgttggcc accgcctccc gcatcgccat tccgatccag tgctccatct
    50161 ccgcgtcgat tccccggagg cggcagacgt gggcgagcgc cgcctgagcc agctcgatgt
    50221 tctcgtagcg gctcgaaagc cagaggacga tggggccggg cgctgctgcg ggcatcgttt
    50281 cggtctgtca ggacgcggca ccgagaccgg gagcgacggg gtcaggggcc ccgcccctgc
    50341 taagctgcgg cgccatgtgc gcgcccgagc cctgcgacga cgtcgccgaa atcatacgcg
    50401 accccggtcg gcggcgtcca ctcgggaagc cgctctcccc cgtcgtcggg ctcctcgtcc
    50461 tcgccgccct cgccgccccg cccgcacgtt ccgacgattc cgccctcctc gccgtccggg
    50521 tcgcgagggc ccagacgctc gtcgagaagg tgaggggcgc cgccttccgg gccccggtcg
    50581 cctccgctct cctcccggag aagcagctcg agaccgtcct cgcgaagaag ctcgtccagg
    50641 acctcccgat cccgttcgag gcctacgccg cgggactggc cgccctcggg ctgatcgagc
    50701 cttctcccgg gctcctcgat cgcctgattc gcctctacac ccgccaggtc gtcggcttct
    50761 acgacccggg cgagaaacgc ttctacatcg tcccggagcg gtcccgggac gtcgccggcc
    50821 cggcgggcga gctgatggag cagctgctcc tcgctcacga gctgacgcac gccctccagg
    50881 accagcgtct cggcctcgac cggaggatga aggcgcttcg ggactcgacg gacgccctcc
    50941 tcgccctcca ggccttcctc gaaggagagg cgacggtcct catgaccgag gccctgctcg
    51001 agagcgttcc cgaagaagcc cgggaggccc tcggagccga tcccctcggg caggtcctcg
    51061 acggcctcga cgacccggac ggcgtcgacg gcgccgaggg cgtccccgcg tacttcgtgc
    51121 gggagctcgt ctttccctac gccgccggca ccgcctgggt ccggcagaag aaggcggccg
    51181 gtggctggcc cgccgtcgac gcggcctacc gccggcttcc cacgacgacc cgggagatcc
    51241 tccggcccgg cgtcgtcctc cccgcgcgcc tccgcctcgc tccggccgac cgtccgacgc
    51301 cgcgggcggt cccgggcggc gggaccgcgt cgtgggccga cacgctcggc gagtgggtcc
    51361 tcggcacact cctcgagcag gccggggccg gcgacgagtc ccgcgacgcc gcggccgcct
    51421 ggcaggacga ccggatcgtc ttctcttgcc ccggaaagtc gccgggcgcg catggcgtcg
    51481 gattcctctg gcggatccgg gcggcgacgc cggcgggcgc ggcccgcatc gcggagctcc
    51541 tcgggccgct ctacgcgacg cgtcccgctc cggcccggcc ccggatctcc gttcgcggcg
    51601 atctcgtcga agtcgtccgg agcgtggtcc ccccgccccc cgcctgagcc tcagcccggg
    51661 atcgcccgga ccgcggacgg gaggacgagg agcagctgga ccgcgtcgaa cgtcccgtgg
    51721 gccaccatcg gggcgaggac ggagcccgtc ctctcgaacg tcacggcgag gaccgtcgtg
    51781 atggcgagga gaccgacgaa gtagagcggg tcgccgtagc cgccgtggga gacgaggaag
    51841 aggatcgacg cggggaccgc cccgagccgc cgctggagga agcttcggaa gaagacctcc
    51901 tcgacggtca tggccgccgc caccaggacg agccggctcg cgaagggctg gctcgcgagc
    51961 cagcggacga gcggcgggat cggccggacc tcgtccacgc cggcgaagcg gaggacgccc
    52021 agcacgacga gcccggcggc gaccgtcagc ccccaccccg cgagacccac gacgagaccc
    52081 gtccggacct ccctccgcgg gctcgcggac gtgagcgcga ggaactccgc gagcggcggt
    52141 cgccccgcga gcagccacca cgcgacgagg aagaacagga ggagctgctg gagcgcgagg
    52201 atcgacggga ggctgaggcg ggccgggtcg atcgggcgac cgagcgagaa cgccggtagg
    52261 atcgtcgtgg ccgcgagcgt gacgccgagc gcgacgagcg caccggcccg ccgccgatcg
    52321 tgagggaact cgtcccgccg gaacgcgccg cgccgcgccg cgacgacgac gatcgccgcg
    52381 acgatcggga gcccgacccc gaggaggagg acgaggtcga tcggcttcac ggtccgctca
    52441 ccggcccgct tccgggaccg gacgaggatg cgttcgcgac gtcgaggaag aggagtgggc
    52501 gttcatcccg cgagcggcga agtctagcag cgcgtcgtgg agctccggca ggggctccgc
    52561 gacccgcccg gcgcgatccc gccggcggtc cgcggcgacc cgggaaggcc ggagacgaaa
    52621 aacggcgggt cttcccgccg ctctccaagt cctcgtcgtc tctcgaaatg gcggggccga
    52681 cggggctcga acccgcgacc tcaggcgtga caggcctgcg
//
LOCUS       DAOONL010000016        52283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_447_length_52283_cov_16.512560, whole genome shotgun sequence.
ACCESSION   DAOONL010000016 DAOONL010000000
VERSION     DAOONL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 52283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_52283_cov_16.512560"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4544
                     /locus_tag="PLP50_04360"
     CDS             <1..4544
                     /locus_tag="PLP50_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPA50815.1"
                     /translation="PPAPSGHAIEVRLNAEDPDAGFAPSPGKFLHFRLPTGPGLRIDR
                     GVTEGDTVAPEFDPMIAKLIARGADRGEALARLRRALSESAVVLDGGTTNKAFLLELL
                     DRPEVRSAEVDTGWLERWTAERAAEERPHAAIALLAAAVEAYDDELRVEEEKFYQSAA
                     RMRPEIRKEVGRTIEFRHRGLNYKLRAFRLDRHDYRVELDGRKCELRVEPLSPYERWL
                     HIGGVRYRVLCVVQGLEHLVEVDGVPHRFSRDDLGILRAPSPAVVVSLGVSAGARVSR
                     GQRIATLEAMKMEMPIPAPFDGVVRSVFVVPNVQVGPGTPLVQLDPAAPADAAAPAAS
                     RIALPNGPAAPVAVRPLAILDEIRRLVLGFDVDPADARRMTREYVDACPALASDPECL
                     EAEERILRLFADVSGLFRRQTASEDPDAEGVVSTGEYLLTYLRTYDTQTPDLPPRFVD
                     RLSRAAAHYGVEGTRKVPALREALLWIWKSRQRAEHQVEPLMAILERRLAAASSGAVG
                     GSPELRSLLDRLVSVTEGRWPALGDVARDLRYRVFDQPAFEAARRAVYTEMERVFDRL
                     AADPSSPEREDGVRRLVECPQPLQSLYMSRFEGASPAVQGLMLEVLTRRYYRIRTLEG
                     FREVSFGGRAGALAEYAFEGVRHLVVTTWCPDDELDAALRDVAAYLAEHPSEHDVLVD
                     VYSWRPGPLGEPDAKAAEVKRVADAAGLGPRVVRVVLALSAAGAGLGMGGMQHFTLRP
                     GDGGFVEEKLYRAMHPMIGKRLDLWRLEGFDIERLPSAEDVYLFKGVARENPKDERLF
                     ALVEVRDLTPAFDAQGRFVGLPQMERMLLEAFEAIRHVQAHRRPEDRLQWNRVFVYVW
                     PPFTYGATVVRAFAKHLAPAAEGLGMEKVAIQARIPDPAVPGGLKEVVFSVSQPGRTG
                     ITVSVTPPSRKPLRTLSEYEQKVVRLRQRGLTYPYEILRMLTPPKDDAHADFPPGDFV
                     EHDLDEEGALVPVSRPPGKNRANIVAGLLTSYTAKVPEGMTRVVLLGDPMGAMGSLAE
                     PECRRILAGLDLAEKMGVPLEWYALSAGAKIAMDSGTENMDWISRVLRRLITFTQAGG
                     EVNVVVCGINVGAQPYWNAEATMLMHTRGILVMTKDGAMVLTGKTALDYSGSVSAEDN
                     LGIGGYERVMGPNGQAQYWARDIPDACRILLRHYDHTYVVPGERFPRRAETSDPIDRD
                     VCLSPHGGDEPGSFALVGEIFSDATNPGRKRPFEIRKVMAAAADQDHAPLERWAGLRD
                     GENGVVWDAHIGGWPVCLLGFESHPVKRIGFVATDGPENWTSGTLFPQASKKIARAVN
                     AASGNRPLVVLANLSGFDGSPESMRKIQLEYGAEIGRAVTNFKGPIVFTVVSRYHGGA
                     FVVFSKTLNDNMEVAALEGTYASVIGGAPAAAVVFAREVDQRTKRDPRVAALEREMAA
                     ASDVERRKLRSRHADLVAAVRSEKLGEVADEFDRTHSVQRALAVGSLDRILPARELRP
                     YLVDAIERGMAKEMKRIGR"
     gene            4838..5215
                     /locus_tag="PLP50_04365"
     CDS             4838..5215
                     /locus_tag="PLP50_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPA50816.1"
                     /translation="MAPAGARLGLDLERIEARPSSFLEDWFTPSERAFVGASPAGEAA
                     LRATLVWSAKEAVMKALREGLRIPPRDVETAPAPGPADGLWRRFAPRGPGSAEWSGWW
                     RADEGFVLSGVADPPSGPPERIG"
     gene            5360..5731
                     /locus_tag="PLP50_04370"
     CDS             5360..5731
                     /locus_tag="PLP50_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPA50817.1"
                     /translation="MIGPNIRIQGELSGDEDLVVEGRVEGKISVSKGLRIGPQAQVNA
                     EVKAHHVVIAGRVVGNVVAAEKVEILPSGILEGNIRSPKIAIAEGAQFKGSVDMSGKA
                     APATPASPAPAGDKKDGSAKG"
     gene            5818..6810
                     /locus_tag="PLP50_04375"
     CDS             5818..6810
                     /locus_tag="PLP50_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50818.1"
                     /translation="MTRSVRVSVLLLALASLAGCASSARPRATALAAAGGTAARVATE
                     SMRETEASVDRYVEAQLLLAPLTGRPEPGEETLLTLRRVRTVLAARSAAMAELGAAYA
                     GLDALAAFDSAGTVEKGVDGLVGAVSAYREALGQPAIAPVPAFGLSKGLGAFAGARQA
                     ARLKEASAAIRESLERFVALLRKEQEVHASLRRALVEGQGSAARAFAALGLGRPGALL
                     APHAAAFGLAWDEKEYDGALSLLRKEPSITGRPGASREDDLRAALDRVLEHRVARRVD
                     LEAALVDETLAGLEALVLAHRSFEAKRETDLAAVLEHVAAVRALLDEYRAAAGK"
     gene            6822..7169
                     /locus_tag="PLP50_04380"
     CDS             6822..7169
                     /locus_tag="PLP50_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50819.1"
                     /translation="MARTKSNHERLLATVEATYRLAERSFDALHAAAKGAEERERVRE
                     LYGAARDAYWKAVLACLEDQSGVVAAIHDELKAKNRELKRSLETLRDLASTLALLRDA
                     VRLAAALVTLAAA"
     gene            8054..8437
                     /locus_tag="PLP50_04385"
     CDS             8054..8437
                     /locus_tag="PLP50_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA50820.1"
                     /translation="MGRVALDASAILVLLNDEPGAAAVVEAIEDAVVSAVNLSEVVGK
                     LVETGMPRQVAEDALGGTGLEACPFDEEAAWNAGELRTGTRKAGLSLGDRACLALAKE
                     LDLAALTVDSAWKKNAAGVEVRLVR"
     gene            8632..10182
                     /locus_tag="PLP50_04390"
     CDS             8632..10182
                     /locus_tag="PLP50_04390"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HPA50821.1"
                     /translation="MSASRALVLRLVAIGLAAGAPALATAAGSSGGEPPRHHFGDVAR
                     PSVALTFEKPETAGAPCHLRLELLAGNRVEVRAATPAPTRATCRRTEDGWEVSGMTRL
                     EGKELAFRASVRPDGSALEWTSPRSVTLRDGSVHALAGRYPRLGEEARAAAARAAFGA
                     VDRELNAVYRRLVAARPPESVAALRADQRRWLKYRDFTLVDGDDAAAVGPGTATHLRQ
                     QALRTLERVLYLGALLDPPRTALGETLYSDGRGGTIAVRRLGDALTFAVRADLPGFDL
                     PKVPRDVPLRLGGVARPSGPSAWEALPEAMTASDGTWAPERLRLSLGADGRLRVEAAG
                     GETGPLARVLTGDDAPLRRLAPADSPLRALVADLPAQAFDDTTDGLEGAERQALLTTG
                     AGGAFTIGAESPDRLFLRFPGGHVKVALFAGADGGAVLAVEQVNGRNLSVQLWRQAAW
                     GEPFVAYPEGLAALGRGVLHLDDSGTLRQTRSGSGSGRASDDTRVLAWDGFGFAAERA
                     DAAAPAGK"
     gene            complement(10298..10957)
                     /locus_tag="PLP50_04395"
     CDS             complement(10298..10957)
                     /locus_tag="PLP50_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50822.1"
                     /translation="MRARRPLSTVLVLSAAFMAAAPSSADAQSPEPAASDAPRKAAPG
                     SPAPADEFLSRLAGLCGQAFEGRVVADEPAAPGSAFAGKRLVMHVRRCTAGEVRIPFH
                     VGEDRSRTWVLTRTPDGLRLKHDHRHEDGTSDATTMYGGDTAAAGTAVRQEFPVDADS
                     VAAFRREGLTASVTNVWAMEVEPGKRFLYELSRPGGRLFQVEFDLSKPLAPPPAPWGH
                     D"
     gene            complement(11087..12007)
                     /locus_tag="PLP50_04400"
     CDS             complement(11087..12007)
                     /locus_tag="PLP50_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPA50823.1"
                     /translation="MNHSPAASPKPGGSSDVFLFFLSSFIWGTTWLAIKFQLGVVAPE
                     VSVAWRFALASLLLVAWCLLRGIPLRFSARDHARLALLGFLLFGLNYVLVYRAEQHLA
                     SGLVAVIFSFLVFWNLIGARVFFGTPAPPAVALGAVLGVAGVALLFWPEVTSLHAGTT
                     QLRGLVLALVATLFASAGNLYSQRLFSTGLTVAPSIAFAMGYASLAVAAWCAVSGIPF
                     TFDARPGYVLSLAYLALFGSVVAFMAFLTLLKRIGAGRSGYTAAVIPLIAMLASTAFE
                     GYRWSAAPVAGMLLVVGGTVLVLRAKERVA"
     gene            complement(12115..16488)
                     /locus_tag="PLP50_04405"
     CDS             complement(12115..16488)
                     /locus_tag="PLP50_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPA50824.1"
                     /translation="MSETRPGSSRRIKPLPLLIAAVTVLSLSLPGGLGGLFGEPGGAA
                     TGGALSATAPATVFVPAAAHASGANGASWRTDLEVHNPGTTAAAFAVKLLVRDADNAS
                     SATKSYTLPARQSRRFTDVLKTEFAFDGAAALRIGVTSGSLVVTSRTYNLLGANPWGL
                     PQGASFSQFVPGIEESEAIAYGQEGRLIHLTQQPSATLNGFRTNLGIVNATASPIDVT
                     VDLYRANGAFLGRRQGAETRLPALGFRQLNEVLGEWGTVADGYAVVKTTTPGGKLFAF
                     ATVIDNHNSGDPVFVPATVLTGAAPAPTPTRTPTATPTPPAGGYPNLYFYRPATWPAC
                     IVVHSASGCCTSSACCSPAPVSGSSAFVQIFLANSGTVRLTGPVRLSLAIDGAVAGYA
                     NWANDSGLEPGYGIVLTWEFTGPVAAGTHELRLTIDPDDTISETNGADNVCVTTATWM
                     GAPLLYEGAAPDGSGAEDELGAGPPEPLAEALPRQAALAGSGPIYVPASAHASGVNGA
                     SWRTDLEVHNPGATAASYTVTLLRQGQDNGSGAPVKSFSLGPQQSVRYADVLSSVFGY
                     TGAAALRIATTSGSVLANSRTYNLIGANTVGLPVGASFGQYVPGLAESEAIGAGQEGR
                     IIQLSHRDSGTLNGFRSNIGYVSTTGTAIELRIDLYRSDGTLLGTIQDERTRLAPYGF
                     GQINAVFGAWGSAIDEGYAVVRPVTAGARFFAFATVIDNHLTGDPVFVPATRLAATAL
                     PGTPTPTPTATPGTGVITGPGGTTVTLPPGATTSGGTVTLTSGDGSTLAKPGETLVST
                     VVKTNVSGTGVAIGNGSFTVTLPVSGTVADPEKLLLKVAVSTGRVYPVAGAYDAGRKT
                     FTAELMKVWNGWTMGVVARPSLAITSTTPLGSGGISSLGWVTPTDWQTCAFRVFDAAA
                     SASAGFKTGVPAAMKKACDHLRGAEFRSPRLWVDGRWNPKARAVHLVQGTGPNDPVTS
                     FSQTLDDAAFSMAGYTDAQMQSLGQMYFNWDDWNTSIQPSGFSLENVSIHELFHAVQA
                     GYDFRDRWFQSGGTWMHSAMSMTEGTATLVGQMFQSNLNGLYGGEVTVRSPEPPQRLE
                     QDFHWPTGVAAYARQDFFAYVAKRWNGGVLRDLRWLFQHLSDQTEGQFGKPADEYYTL
                     YRKAMDFHYQSTFGLSLPDLYTEFVLDRAYRHDEPALLRAADRSLKKNSLDPTLFPAA
                     LAWDPATTPRAELADIDPLSARAVRVTVSDAERTAKKFTTNVAVTGTTFGKGGVRVFV
                     FPEKDGVLQKGLDLEVADIQKPVEVPIDVETATLTVLVVNVSIDHAVAKVTLGGDLTR
                     ISLGDSIIYMCPPDAPPTGCPSGRCSVKSNGESSFIFPLVWAGNRFEVNFQNVATYQG
                     ELSADRKTLLWFKADGTWSFKSHWINLPLDPVRSVSGRLVFAVAGEAARPHIVFEQSC
                     GTGYPTIDFNQPLKLEVWLAP"
     gene            16686..17561
                     /locus_tag="PLP50_04410"
     CDS             16686..17561
                     /locus_tag="PLP50_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPA50825.1"
                     /translation="MSDLSPATPIDARSRDIGGFTVRRLLPWRARRMVGPFVYFDHMG
                     PVALEAGRGVDVLPHPHIGLATVTYLYDGALLHRDSLGSEQVIRPGDVNWMTAGRGIV
                     HSERTPPAERASGPRLHGLQLWVALPTADEEIQPSFRHHPGATLPETERGGVRLRVMA
                     GSAYGATSPVEVRSPLFYVDVAMPAGSELPLPDEHAERAVYVVEGSLRAAGATAGPGR
                     MLVFAEGADVTLKAEAATRAVLVGGAPLDGPRHIDWNFVGSSKERIEKAKADWREGRF
                     PNVPGDETELVPLPG"
     gene            complement(17653..18918)
                     /locus_tag="PLP50_04415"
     CDS             complement(17653..18918)
                     /locus_tag="PLP50_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPA50826.1"
                     /translation="MAGWLVPLRDLTPDQLRAVELPADAHRVLSGGPGSGKTLVLLHR
                     ARRLADTLRIPEGRLLVLVYTNALTAFLRSALAELGIPESAVRTFDDWCAGHYERFVS
                     AEKPWDERRGRPRFDAIRDGVLRDVRRHPDRPPLYDAVLVDEGQDLDAHAFETLAAVS
                     RHLTVALDPRQRIYDGGAREEEILAALGIPSRSVALLDAWRCSPCVSRLASRLLPEGD
                     ARQAFLRNALAGPVEREKPLLYLAGGHADEIVRLADVTRARLLRGERVAVLLPGRRLV
                     ASLACGLSRLGIPVEVPHRPGTRPGPFPPLDFASDLVKVMPYHSAKGLTFDSVLLPRL
                     ASSDVLARGASSLLRLLFVGITRATSWVYLSAVEGDELPLLHRLAASAPEVVVQRHGA
                     HAAPPPAPGGTGDGPGATPGSDAGLTDWL"
     gene            complement(18930..21272)
                     /locus_tag="PLP50_04420"
     CDS             complement(18930..21272)
                     /locus_tag="PLP50_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPA50827.1"
                     /translation="MRVLGIDLGTTNSTVTEAVAGPGGRTLTTLEVKQETPEGEYVHV
                     LVPSVVAIHRGKTWVGEGAKRLRARAGELGLSPSRDLFWDTKNDIGVRKTYHRAPEGC
                     RNASEIGAHVLRFLAAAAARKGAPPPDRVVVTVPASFQAAQRADTLAAARAAGLPVSE
                     GDLLDEPIAAFLDHADRNGLDALGRAGETKHLLVFDFGGGTCDTAVFRVTLAAPGEPL
                     RAATLAVSRYHRLGGGDVDAALVHQLLIPRLLEENGLGRHDLDYEEKARVVVPALLGV
                     AEALKVGLCSEIRRLRGLGRWEAADPNEVAKTLPGSYPCRLPDGRDLRLTSPRLTTRQ
                     LDGVLFPFLDEELLYAKGDEYRTTCSIFAPIEDALSRGGLSAGEIDGALLVGGSSLIP
                     QVVDAVARRFPSARVLREETPEAVQSSVARGAALHALSLATSGRGLLTPVTGGALSVR
                     TASGLVELVARGTPLPFPASGDFAACTELAVPESREREALPLRVELRDGAGGPVMNAL
                     CLFPPPVRQGEPLRLRYRVDANQLLHLRVDLPARPGAGGHELRLENPLTNVVNSQPKR
                     ERLLEIEESLRTGALSASKAAAATEEAARLYADLGQREKALSVYRALLRAKGRPDADL
                     LFRMGLLAGELGDDGREERFYLESAEADPTGSEPLFNLALARRRRGLLAEALSTATEA
                     IRREPDAAYLVLRALVHDSLGDVPARDADLARAFASFAPLPTQGDFALAWYLAACRLA
                     GDAPRLAAAEAEEKRRRAAKPGLPFVDGLLPVLAAFAQTN"
     gene            21790..22098
                     /locus_tag="PLP50_04425"
     CDS             21790..22098
                     /locus_tag="PLP50_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HPA50828.1"
                     /translation="MATPDLLDGCTGFDWDEANVERNRISHRVTFWEAEEVFFNDPLL
                     LRPDEAHSRLERRFMALGSTDAGRLLFISFTVRRHLIRVISARDMTRREARTHAQVKA
                     "
     gene            22079..22369
                     /locus_tag="PLP50_04430"
     CDS             22079..22369
                     /locus_tag="PLP50_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HPA50829.1"
                     /translation="MPKSKREPVPSFASEDQERAFWASHDSTDFVDWTKAEPRSFPNL
                     KPTLRTISLRLPEAMIGQLKVLANKRDIPYQSLLKQFLAERLRQELSKAAGA"
     gene            22391..23080
                     /locus_tag="PLP50_04435"
     CDS             22391..23080
                     /locus_tag="PLP50_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50830.1"
                     /translation="MRCARSPLTLRINRPRNASLLFVFAVALRSARSSRLAAKGRVLP
                     AIVLLASLTAAGCGTRVHEHVLEVVVRDPSGRLGTPPHEVGVFDWRMGRSAEWARKTA
                     GTAGVRPYRTTFSTVDTVSIGVARPEKVELALWLPGLEREGFYPLRVEPKARPAGNAT
                     LHPVRFDESPADGTARWLAVRWTTAPMERGWRLELDLEVPADAERGRTAERGVGATSA
                     PIPLDPSRPRR"
     gene            23112..23579
                     /locus_tag="PLP50_04440"
     CDS             23112..23579
                     /locus_tag="PLP50_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPA50831.1"
                     /translation="MKRRAGPDRIEQRLERLRTAARGAGLKLTPQRLEIFREIAGSEE
                     HPDVETVFRAVQERMPTVSLDTVYRTLWALHYLGLVTTLGPQQNGVRFDANLEKHHHF
                     SCVSCGLVRDFESPKLDDLPLPADLKSLGSVLNAHVEVRGLCTRCEAEKKTDS"
     gene            23633..25843
                     /gene="katG"
                     /locus_tag="PLP50_04445"
     CDS             23633..25843
                     /gene="katG"
                     /locus_tag="PLP50_04445"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPA50832.1"
                     /translation="MSESGKCPFTGASSHPVAGRGTTNRDWWPEQLNLGILHQHDPAS
                     SPMDPGFKYAEEFGKLDYDALKKDLYALMTDSQEWWPADWGHYGGLFVRMSWHSAGTY
                     RVSDGRGGGGTGNQRFAPVGSWPDNGNLDKARRLLWPIKQKYGNQISWADLLILAGNC
                     ALESMGFRTFGFAGGRADIWQPEEEINWGREKTWLATSDMPDSRYSGDRQLANPLAAV
                     QMGLIYVNPEGPDGNPDPIASGRDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDP
                     KLVGPEPEAAPLEQQGLGWKNALGTGKGVHTTTSGIEGAWKPNPTKWDMGYFDMLFGY
                     EWELVKSPAGAWQWLAKDVKPEHMIPDAHDPSKKHRPMMTTADLALRFDPAYEKISRR
                     FHADPAAFADAFARAWFKLTHRDMGPRSRYLGPEVPSEVLIWQDPVPPVDHPLVDAKD
                     VAALKAKVLASGLGVSEVVATAWASASTFRGSDKRGGANGARVRLAPQKDWEANDPAQ
                     LAKVLGVLEGIKREFDGAAKGGKKVSLADLIVLAGCAAVEAAAKAAGYAVEVPFTPGR
                     TDATPDQTDADSFAVLEPMADGFRNYRKAGTASIEEHLLVDKAQLLRLGAPEMTVLVG
                     GLRVLGANAGGSKHGVFTKRPGVLTNDFFVNLLDMTTVWKPTSDARETFEGSDRKTGA
                     ARWTASRVDLVFGANSQLRALAEVYAQDDAKEKFVRDFVAAFAKVMNLDRFDLA"
     gene            26001..27689
                     /locus_tag="PLP50_04450"
     CDS             26001..27689
                     /locus_tag="PLP50_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA50833.1"
                     /translation="MRPQDQWNDRSTPRRSPRERLASAALLAALLVPGTGGGQEPEKL
                     PRVRGSVELSLVNVDVAVTGKDGRPIEGLSASDFTVVHGKAPVAISNFREERLVPRAE
                     VATAAPAPAPVAAQAAAAIPEAPPARRHVILFVDHLALPDKREREQVFGSLKALVRKT
                     VRPGDGVMVVSWRRGVRTAWPYTDDVAQIERALDLVAGGTIRLDRDAQNDLDQFAATE
                     AFQAWAGEPPDTSLSRDLSVEQAYNELKGKASAVMGLVSTMAGLEGKKVLVLASRSFS
                     RRPGVEYSGHRVETGRLLDEVAARANAAGVTIHALYAAAWQPDLPNVTDSRFQPPGAA
                     GSRSATGRSDARLEDLAGLETLSGRTGGVLVGTTMEASLFADKVSSDLVHWYSIGYPV
                     PSGASASARISVRVNRKGAKVRTRREVIDRTPDQRMEDRVLANLFRADLSGRLPIGLS
                     VEPARREKERYVRTATLRVPVKDLVLLPEAAARRGEVKVFWVVAEPNGEFSDVRSERH
                     EVAFPPEESAPGPETELRLEVPIETSGPEARISLGVRDEIGGEAGFGLIRPAAR"
     gene            27873..28760
                     /locus_tag="PLP50_04455"
     CDS             27873..28760
                     /locus_tag="PLP50_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HPA50834.1"
                     /translation="MRSKTTGRALRARCRAARVAVLVFVATSAPRLAAGEGAESAGGM
                     ELPSIVETCPLFRSDGGAEGSFGFGEGAFGGEQSAWRTEPVSGFSLCPRLEATRVAER
                     PELRWAPWTARDPREPLRGVRNATAPHRKRLVRSTVILTGTAIASGAILSLLPKEETN
                     WEEGAFDHGLSNLGRAWTELPVWDRDGAFHDWFGHPYAGAFYYNMMRSQGATVSQSFA
                     YCVAQSVLWEYVLEAAAEQPSIQDLVSTPIMGAALGEALHRWSISILKKGRLNFGEKV
                     LVFLLNPSYVINNGYRAPE"
     gene            complement(28789..30558)
                     /locus_tag="PLP50_04460"
     CDS             complement(28789..30558)
                     /locus_tag="PLP50_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPA50835.1"
                     /translation="MAARAALPFPRAAREGEAREGGAMETLTLLGTTLGLSFTAGLNL
                     YATILVTGLAVRFDWLTLPAGLEGLAVLSHPAVLVAAGLLYVVEFLADKIPAVDNVWD
                     VLHTFVRPLGAVLISWAAVSGANVPKPLEIPLLLLAGGVSLSTHAGKAGTRLASTATG
                     GHATGVGVGLSLLEDVAAVSIAPLALAYPVVTLVLVVVALALLALVAPLGWRLLRSRV
                     AALLYLFQHKVVDRELPGLDTSHLPEDVALAFTDAGVRSGSDGLLLPAYAASLPGVPR
                     WIPGYVALVPGGAVFACRGWFRTRVGRLSWSDGAPLLVRRLTGYVLHARTPKRRATIT
                     FFPLTKPMGQALERAAALAASARPSGGRDRRAGLTGLTRFTLRRGDNPPMRRLFVVLA
                     LALAAAPPLRAQEVRIVELTPFLGARSGGRLKDFGTGQWFSVRPSWSWGFVAGIAPGR
                     PNLIFEGTFLRQATRLDAENAFLGGDGNLHDLDLETGLAGVQWDFAPKARVRPFVSGG
                     VGATSFEAQGGGYTTSFTASLSGGVKVMTTRGFGLRVQLRALGFFSGSSRRDLCRWSS
                     CEVAIPSNGTYQVDLSAGTFFAF"
     gene            30601..32667
                     /locus_tag="PLP50_04465"
     CDS             30601..32667
                     /locus_tag="PLP50_04465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPA50836.1"
                     /translation="MKTARTPIALALLTLLAGSAALAEAPAAMAPLGLADLFRVRVAR
                     DARISADGKAVAFLVQVPRTPGKGEDGPAWTELHVAERGKAPRPYVTGEVNVSRVSFS
                     RDGSELFFVTKRKGDSTAALWSIRRDGGEARRRVALPADVEGYALSPKEDGVVALLAK
                     EKKAEAVEAREKKGFDREVFEEEWRPAKVWVAQLDGDAPPRALDLPGSASDLRFAPTG
                     DRLAVALAPTPSVDDGYMNRKVHVVTLDGKVVGRVENDGKLGSVAWSPDGTRLAMICA
                     ADRNDPSTAALGVVPATGGRPKDLLAGFDGHPAAFAWAGNDRLVVVAARGVATELLEL
                     PAAGGAPKRLPLAEGLVVDALDRAPDGTLAFVAERPDRPAEPFLLAAGSAKPEPLADL
                     NPWLAQRRLGKQSVVRWKARDGVELEGILVEPVERAAGARVPLVLLVHGGPEAHVSNG
                     WLTRYGEPGQLLAAKGIAAFWPNYRGSTGRGLAFSKTSQGDPAGKEFDDLVDAVDHLV
                     ASGLADPKKVGIAGGSYGGYAAGWGATFYSERFAAAVSFVGISDLTSKAGTTDIPVED
                     VDVHMLSMPWTRFEQSRERSPLSYVAKARTPLLLLHGTADPRVHPTQSLLLYRYLKLA
                     GHPAVRLVRYPGEGHGNRRAASQLDAAVRILGWFEHYLLGAGGAPPAPEAADGTVLEV
                     VEAAKE"
     gene            32783..34084
                     /gene="glgC"
                     /locus_tag="PLP50_04470"
     CDS             32783..34084
                     /gene="glgC"
                     /locus_tag="PLP50_04470"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPA50837.1"
                     /translation="MEETLAFVMAGGQGERLRPLTDVRAKPAVPFGGIFRIIDFTLSN
                     CVNSGLRRIYVLTQYKSYSLSSHLRTGWNMLSPRLNQFIDEVPAQQQLGSSWYQGTAD
                     AIRQNFSFVESKRPRLVLVLSGDHVYKMDYRLLRAFHDEKGAELTVACVRLPAEEARG
                     TFGVMQVDEEGRIVGFEEKPAEPKTIPGTTDCLASMGIYLFEAGCLRRGLDNDLLDFG
                     KDVIPLLLRDGVPVYAYDFTTKNAIPEWEYTKRDGVRRKELVPRASDADYWRDVGTLE
                     QYWLANLDLVQPKPRFNVYGERFPLYSAPGHFPPAKFVHEIPGRTGVAVNSMVADGVI
                     VSGGTVRESVIGSGVYVHSWAMIEKSVLLGGALRGGVHTETSIGRRCRVKNAIIDKNV
                     RLSENISIGYDRDDDERRGLRTVPITGSSEHIVVVPKDFSL"
     gene            34152..36623
                     /locus_tag="PLP50_04475"
     CDS             34152..36623
                     /locus_tag="PLP50_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPA50838.1"
                     /translation="MGWGRTRKSSGGESAPRPAERMVRVGCPAHNCGGRCVLLATVRD
                     GRIVRLEADDRPDLLEAPQLRACARGRSYLRRQYHPDRLLHPLKRVGKRGEGKFERVS
                     WDEALDLVASEMRRVRETYGPGALFVPYGTGSYNQTNGSHVARRLMNVAGGCLGIWNS
                     YSWAAINVATPTVYGTLTTGNQRQDWLNAKLILMWGWNPAEMRDGTNSDFFVKLARER
                     GARAVCIDPRHSLSAAALADEWVPIRPGTDAALMTAMAYVMVTEGLHDEEFVRTHCVG
                     FDASQMPAGCEGEESFLAYLLGTKDGVPKTPEWAEAITTVPRETTARLAREYATLRPG
                     VLYQGYGMQRRAYGEQVVRAGCVLAALAGNVGVSGGWASGLGNQAPDGGPSWTVFPTG
                     ENPFGASIPCFLWTEAVLRGSEMGEADGVVFSGSVARGRDAAAVLAGGRVSPPPGVEE
                     ESPAPPRRLGTDVKLIYAVATNALVNQHANVNRTARILADETKVECIVVQDNFLTPTA
                     RFADVVLPACTQFETWGVEDGWKYGDEVILMPKVVEPPGECRSDYSICADLARRLGVE
                     EAFTEGRDERGWVEWVLDRYREGRFPDLPPLGELLEKNVGVWAKPVTRPAVAFAEFRR
                     DPEAHPLDTPSGKIEIFSKALHEKGRPDEIPAVPKYVEERESPFGPEAEAYPLQVIGH
                     HTLHRVHSTHDNVDWLEEAFPQRVFLNPLDAKARGIDDGDEVRVFNARGAVRLRCRVT
                     PRILPGVIDIPQGAWWTPDERGEDRRGNVNVLTSERWTPLAFGTAQHTAMAQVERVAG
                     PEGARPRRRRPRPAAAAFRRLERPR"
     gene            36620..37237
                     /gene="dmsB"
                     /locus_tag="PLP50_04480"
     CDS             36620..37237
                     /gene="dmsB"
                     /locus_tag="PLP50_04480"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016458576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HPA50839.1"
                     /translation="MKGPYAVHFDASACSGCKACQAACKDHNDLSEGILWRRVYEVSG
                     GGFRKDGAAWRQDVFAWSLSLSCNHCEKPVCAEVCPTGAVRRREDGVVLLDSGKCMGC
                     RYCEWACPYGAPRFDEARGVMTKCTLCADRLEDGLAPSCVAACPMRALDFGSLEEMKE
                     RYPAPTGFPLPDPGLTEPCFRMTPHPAAARSEWEGEIANVEEVER"
     gene            37234..38085
                     /locus_tag="PLP50_04485"
     CDS             37234..38085
                     /locus_tag="PLP50_04485"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HPA50840.1"
                     /translation="MREASLVAFTLLSQAAVGICGVLLALALRAERAGTSAALEAVAL
                     PLLAAALVAAVLGLAASFLHLGSPRNAWRALRNVRTSSLSREVFLASLFTAALAAAVL
                     VRLRTDPPGFAAGFAEGFASVLGVGLVMTMAGAYRLRTVPAWDRVATPLAFFGSALVL
                     GKLSVAAALALRGPDAASGACTVRLVIGGLAVLLAGLGVTLFWLRRIGRERGASREAF
                     DRTAGASRRLVAARIVLTALAAGAGLGAFAGELAAWFVVATLVLAFAAETAGRVLFYE
                     ARVRSGL"
     gene            38216..38845
                     /locus_tag="PLP50_04490"
     CDS             38216..38845
                     /locus_tag="PLP50_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPA50841.1"
                     /translation="MKTVLALVFALLPFSSAFGQEPEPLPAARRSGGMPLMEALAKRS
                     TSRAFDAKPLTTQQVADALWAALGVNRSDGKRTAPTSRDRREMEVYVLRSSGVSRYDA
                     SAHRLVPVASRDLRTVQSAQEFVYDAPVTFVYVADLSKMSDGSRAAKEATASFDTGFV
                     SQNVYLWCVSEGLATGVRSWVDRDVLAKGMKLGPDQWIVAAQSIGWPKR"
     gene            39014..39421
                     /locus_tag="PLP50_04495"
     CDS             39014..39421
                     /locus_tag="PLP50_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPA50842.1"
                     /translation="MLNRLLAPRTEAAYALLRVVAGVLFALHGAQKLFGVLGGTVQPA
                     GTQLWLGAVIELVAGLAIAAGAFTSWAAFLASGTMAVAYVQFHWKLAFDAGFFPVVNK
                     GEMALLYALLFLFVACRGAGPCGWDGRMGSKES"
     gene            complement(39337..40518)
                     /locus_tag="PLP50_04500"
     CDS             complement(39337..40518)
                     /locus_tag="PLP50_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPA50843.1"
                     /translation="MPLRVLFLSDTHLGLDLPGRPRVDRPRRGDDLFASFERALEPAI
                     RGEAGVVIHGGDLFYRSRVPAWLAERVFARLADLADSGVDLFWVPGNHERGAIPRELL
                     LTHPRVKVFDRPRTFVVRRDGVTLALSGFPYAPCVRDIAPALLAETDADSVRADVRLL
                     CLHQAIEGATVGPSDYVFRSGVDVLRGADVPPAFAAVLSGHIHRAQVLTKDLAGRPLA
                     APVLFAGSTDRVSFAERFEPKGSFLLELSSDGSPGGRATWTFREHRVRPMAVVEIDPV
                     PPGLSERIDAALARLEPRSLVRLRLLAEPPAESLPLLRPEALRARAPAGFDVSVAWPR
                     AEGASTRAAPAPVPAARSGRSRRAAPAGDAEAQLSFEPIRPSQPQGPAPRQATNRKRS
                     A"
     gene            complement(40630..41208)
                     /gene="nuoE"
                     /locus_tag="PLP50_04505"
     CDS             complement(40630..41208)
                     /gene="nuoE"
                     /locus_tag="PLP50_04505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPA50844.1"
                     /translation="MSEHAACTDNWEEVKATSLRILPSHIVEFIEKNAKTEHPESNLI
                     ATLHMVQAHFGFLGQTQMHAVAQLAQIPYATVTGVATFYHYFRLQPRGKHVINVCLGT
                     ACYVKGADKLVARLSDDLGVHLGETTKDQMFSLESARCLGTCGLAPVMMVGDQVYGPV
                     TPAEASRILDKYLKEEKGKAAPREEPAAAHVS"
     gene            complement(41205..42977)
                     /locus_tag="PLP50_04510"
     CDS             complement(41205..42977)
                     /locus_tag="PLP50_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPA50845.1"
                     /translation="MPTSQKMINLKIDDEFIQVPAGTKVINAAEKIGIHIPRLCYHPA
                     LSIEGACRVCVVEVKGFNHFLTACSQEVWEGMEVQTNSPEIRQARRDIVELLLDNHPM
                     DCQTCDRDGNCELQKQAYRLGIRERLFAGKRKQLPVEDSGQSVVRTPEKCILCGRCVR
                     VCSEVQGVSNLSQHGRGFHTVVGPANLANMDESVCIQCGQCVAVCPTAAFLEKDHTER
                     VWEALALPRDVKHVVVHTAPAIRATIGECFGMPVGTPVTGKLVTALRRMGFDGVFDTN
                     FGADLTIIEEANEFLGRFQRDENLPLITSCSPGWVNFLEKFYPEMIPHTSTCKSPMQM
                     TSTLLKTYYAEKKGLDPKDVYVVSIMPCVAKKFEAARPEHRMPDGTPHTDAVLTTREL
                     AWMIKSYGIDFESLPDGDFDRPLGISSGAADIFGATGGVMEAALRTAAVKLTGKELGP
                     LVFEEVRGVKGLKEATVHINGLDVNIAVSNGLTNAKAILDAAKAGGKKYHLIEIMACP
                     GGCIMGGGQPYPPLDEDPMDPEIAKLRARALYDIDSHKQLRRSHENPAIEHLYEEFLG
                     EPNGKKAHELLHTHYQARMPRGIR"
     gene            complement(42994..45171)
                     /locus_tag="PLP50_04515"
     CDS             complement(42994..45171)
                     /locus_tag="PLP50_04515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPA50846.1"
                     /translation="MPNAVTQAFQSLAARSEEALALRGDAGKVVVQIGSATCEHAAGS
                     REVADEFVKHLASSGREDVVLHRTGCTGRCSKEPIVGVSVPGKPPVKYERVDRELVHK
                     IFTSHIQKGAALPEHALDGSLETPYEYELVFCEGRRCKQRTELKASFEAKLAAAGIGP
                     DRVKVNSHACLGVCSEETEGKSTHVLVRPSKTIYRLENEKDLDALLDGPVRKGTIDER
                     MKVPFKPIAQDFFDLYADVRFFNAQSRIAMRNAGILDPESLEEYVRFGGFKALAKCLE
                     KGDPTWIVDEVLKAKLRGRGGGGFLTGQKWKFARANEEKTRYIICNGDEGDPGAFMDR
                     GMLESDPFNVVEGMIIGAYAIGACKGFYYIRAEYPLAIKRIENALQKCREAGILGENV
                     MGSGWSFDMEIRLGAGAFVCGEETALIRSIEGERGQPRVRPPFPTTAGLWGKPTCINN
                     VETFANVTAIINFGGDWFSQVGTDDSGGTKVFALAGKVKHTGLVEVPLGTPLKDVVFD
                     IGGGVLDDRELKAIQTGGPAGGFIPANRADMPVDFGPLAKAGSIMGSGGMIVLSDEDC
                     MVDIAKFYLTFTQEESCGKCTPCREGTTRMLEILEKITAGNGELSDLDRLERLGKLCQ
                     RTSLCGLGRAAPNPVLSSLKHFRDEYREHIVDKKCRAKKCVALIQYQIDDEKCIGCTA
                     CARNCPTQCISGERKKAHVIDQARCIKCGQCYEICRFDAVERV"
     gene            complement(45711..46655)
                     /locus_tag="PLP50_04520"
     CDS             complement(45711..46655)
                     /locus_tag="PLP50_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA50847.1"
                     /translation="MQRIGTTSLQVFPVCLGGNVFGWTATEAESFAVLDAYFAAGGNF
                     VDTADVYSAWKPGNSGGESEEILGRFMASRGNRDRVVVATKVGKMAGLSGLSAATIRK
                     AAEDSLRRLRIDRIDLYYAHADDPATPLEETLGAFDALVREGKVREIAASNYAAPRLA
                     EALAVSRRLGLARYVALQPHYNLVHRAEYEGELQALCVREGLAVFPYYALASGFLAGK
                     YRPGRTIASVRSGAAAKHLDEPGLRVLAALDAVAAERGTTVASIALAWLLGRPGVVAP
                     IASARTPEQLAEILPAATLSLTAEESDRLEAASRPHGG"
     gene            46780..47790
                     /locus_tag="PLP50_04525"
     CDS             46780..47790
                     /locus_tag="PLP50_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50848.1"
                     /translation="MLRILRYAPVMMFLAGTALGAAPSSQAPAEKDVASSGFAGTWAL
                     AATADDVARVAPANAWGGLGGTTGATARPGGGGFDLPIEVMTDARLLVVEDDGLRLRV
                     TYPSGRVRTFVTDGSKRYVDDGDGPADVVARRRDRTVTVSSEWVRGYRLRETWELRTD
                     PHRLVVTGRLKGRESHEYVRNYEPAPEGAVAASATPPPPAPTPAVRLPWAPSVPASTP
                     APAPEEPGGSPAKEPSSVDRLSECTLRPPARTPPGALNGLAKTPQETAAKAAAASVAP
                     LRADGVLSSDAEVFDGCLVWPFTLRLPALGGVQEVFVDAGDGRVVKSDFIRLGPRPAG
                     AP"
     gene            complement(47831..48748)
                     /locus_tag="PLP50_04530"
     CDS             complement(47831..48748)
                     /locus_tag="PLP50_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPA50849.1"
                     /translation="MRRARPVLLLAAVAVVALVACRRPPAADPAYAAEIGTAWALRET
                     RLASESGWLTLVALHWLTPGENPFGSDPALPLSLEAPGIPARAGAFVLGPDGAVRLAV
                     DAGAPVTVDGAPPTGATLATDRDGKPSVVEVGRVRLTVLDRGGKLAVRARDPESPRRR
                     EFRGIERFPVDPSLRVEGTFEPYDAPREIEVPSAQGPAQKSLAPGLVRFTIGGKERTL
                     EPTVESPDDDTLFFVFGDATNGTETYGAGRFLYATAPKAGPSKVVLDFNLAQNPPCAF
                     TPYATCPLVMPRNVLPERVEAGEKVPAGH"
     gene            complement(48817..49722)
                     /locus_tag="PLP50_04535"
     CDS             complement(48817..49722)
                     /locus_tag="PLP50_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPA50850.1"
                     /translation="MRLAPALLSLLALLVACDRPPAADPDYVREIEAARAARHAELTT
                     EDGWLTIVAREPLAPGENTFGSDPSSRIALEADGIPPRAGAFVVREDGAVVLRAEPGA
                     PVTVNGGAPTDAPLVPEGEERADVVTVGRVRLAYFVKDGAPYVRARDPESARLRGFPG
                     LSHFPIDPELRVEGRWEPYDAPRDVEVPSSRGPARTALAPGLVRFTLGGRELSLEPTV
                     ESPDDATLFFVFADATSGSESYGGGRFLSVPRPAAGTARVVLDFNLAETPPCSLTPFA
                     SCPMALPRNTLPIRVEAGEKAPSHP"
     gene            complement(49752..52043)
                     /locus_tag="PLP50_04540"
     CDS             complement(49752..52043)
                     /locus_tag="PLP50_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPA50851.1"
                     /translation="MEPLFDARTLLAASAVIFAGLVLAVALAWRELRAIRGPDRLALG
                     FGLFFLGLVLLAFRGRIPDLLPDVLANVLVAVAAAFILEGTKLYYGLRPSRRLTVGTG
                     LLATAGFAGATFLRDAGNARAILSSAFLAALLGAAAWTASRRRAPQGAAALDKVTAVA
                     LWACAALFWARGVAVGAGFVEGDILAGGPLLALPPLLCTIFAVVFTTTLLATTSQRLT
                     RVVRTQNDLLASLLSVARAAGAEEGLDGTLAKVLDAAKVATGAHGASALLLDGNGRFT
                     RGLFTEGDSTLVVGPAEAAEMLETGLAGWVVRSGTIAVVEDVLEDDRWRHLASQEGTI
                     RSALSAPIATGTSLAGVLTLVHPDAGHFGEEQRSLIESTAAQIALALRTAESADARRR
                     ATRGLEVLNAVLDVSARRHASEEIAAEAAAAICRLSSFSRVFVALPAADGFLLLGRTE
                     GLGDLRPSTLAGELGLAWETGLVTRISGDGAGQTCRGLVVPLRHLGNRLGVASFEAAA
                     GDGLDDDACSFAEALAEAISLGLGKLVLARAREELSRMLVHDLRGPISGVMGALELLR
                     ENRSLDEDGRKLLDLAERNTRRQLSLVDGILELSRLEEGSLPVHPTDVHLPTVVDEVI
                     GRALPAAQARGLSLVSALPPDLPPVHADPGLVARVVENLLTNAVKFSAPGSGSVRLAA
                     RVDGANVVLDVKDSGPGICEELRPRLFEKFAVGSLPGRGSGLGLPFCRLAIEAQGGRM
                     WLKHPGPEAVFSFSLPLASPPPP"
BASE COUNT         7296 a        18382 c        19532 g         7073 t
ORIGIN      
        1 acccgcccgc cccgtcgggc cacgccatcg aggtccgcct caacgccgag gacccggacg
       61 ccgggttcgc cccgtcgccg gggaagttcc tccatttccg gctcccgacc ggtccgggcc
      121 tccggatcga ccgcggtgtg accgagggtg acacggtggc gccggagttc gacccgatga
      181 tcgcgaagct gatcgcgcgc ggggccgatc ggggcgaggc gctcgcgcgc ctccgccgcg
      241 cgctctcgga gagcgcggtc gtcctggacg gcgggacgac gaacaaggcg ttcctcctcg
      301 agctcctcga ccggcccgag gtgaggagcg cagaggtcga caccggctgg ctcgagcgct
      361 ggacggccga gcgggccgcg gaggagcgtc cccatgccgc gatcgcgctc ctcgccgcgg
      421 ccgtcgaggc ctacgacgac gagctccggg tcgaggaaga gaagttctac cagtcggccg
      481 cgcgcatgcg cccggagatc cggaaggagg tcgggcggac aatcgagttc cgtcaccgcg
      541 gtctgaacta caagctccgc gccttccgcc tcgaccgcca cgactaccgc gtggagctcg
      601 acgggaggaa gtgcgagctg cgggtcgagc cgctctcgcc ctacgagagg tggctccaca
      661 tcggcggcgt ccgctaccgc gtcctctgcg tcgtccaggg actggagcac ctcgtcgagg
      721 tcgacggcgt ccctcaccgc ttctcgcggg acgacctcgg catcctccgc gcgccgtcgc
      781 cggccgtcgt cgtcagcctc ggcgtctcgg cgggagcccg cgtctcgcgc gggcagcgga
      841 tcgccacgct cgaggcgatg aagatggaga tgccgatccc ggctccgttc gacggagtcg
      901 tccgaagcgt cttcgtcgtc ccgaacgtcc aggtcggccc cggcacccct ctcgtccagc
      961 tcgacccggc cgcgccggcc gacgcggccg cgcccgcggc gtcccgcatc gcccttccga
     1021 acggaccggc cgcgcccgtc gccgtgcggc cgctcgccat cctcgacgag atccgccggc
     1081 tcgtcctcgg cttcgacgtg gacccggccg acgcgcggag gatgacgcgc gagtacgtgg
     1141 acgcctgtcc cgccctggcc tccgacccgg agtgcctcga ggcggaggag cggatccttc
     1201 gcctcttcgc ggacgtctcc gggctcttcc ggcgccagac cgcctccgag gatcccgacg
     1261 ccgagggcgt cgtctcgacg ggcgagtacc tcctcacgta tctccggacc tacgacacgc
     1321 agacccccga cctgccgccc cgattcgtcg accggctctc ccgggccgcg gcgcactacg
     1381 gcgtggaagg gacgcggaag gtccccgcgc tgcgcgaggc gctcctctgg atctggaagt
     1441 cccgccagcg ggccgagcac caggtcgagc cgctgatggc gatcctcgaa cgccgcctcg
     1501 ccgcggcctc gtctggggcg gtcggcggat cgccggagct ccgttcgctc ctcgaccgtc
     1561 tcgtctccgt gaccgaggga cgctggcccg cgctcggcga cgtggcgcgc gacctgcgct
     1621 accgcgtctt cgaccagccg gccttcgagg cggcgcgccg cgcggtctac accgagatgg
     1681 agcgcgtctt cgaccgtctc gccgcggacc cctcctcgcc cgagcgcgag gacggcgtcc
     1741 gccggctcgt ggagtgcccg caaccgctcc agagcctcta catgagccgc ttcgaggggg
     1801 cctcgccggc cgtccagggg ctcatgctcg aggtcctgac ccgccgctac taccggatcc
     1861 gaacgctcga gggcttccgc gaggtctcct tcgggggcag ggccggagcc ctcgccgagt
     1921 acgcgttcga aggggtccgc cacctcgtcg tcacgacgtg gtgtcccgac gacgagctcg
     1981 acgccgccct gcgcgacgtc gccgcctacc tcgcggagca cccgtcggag cacgacgtcc
     2041 tcgtcgacgt ctattcctgg cgccccggcc cgctcggcga gcccgacgcg aaggccgccg
     2101 aggtcaagcg cgtggccgac gcggccggcc tcggcccgcg cgtcgtccgg gtcgtcctcg
     2161 ccctctcggc ggccggcgcc ggcctcggaa tgggcgggat gcagcacttc acgctccggc
     2221 ccggcgacgg cggcttcgtc gaagagaagc tctaccgggc gatgcacccg atgatcggca
     2281 agcgcctcga cctctggcgt ctcgagggct tcgacatcga gcgcctcccc tcggcggagg
     2341 acgtttacct cttcaagggc gtcgcccgcg agaacccgaa ggacgagcgc ctcttcgccc
     2401 tcgtcgaggt gcgcgacctg acgcccgcct tcgacgcgca ggggcgcttc gtcggcctgc
     2461 cccagatgga gcggatgctc ctcgaggcgt tcgaggcgat ccggcacgtc caggcccatc
     2521 gccggcccga ggaccgcctc cagtggaacc gggtcttcgt ctacgtctgg ccgccgttca
     2581 cctacggcgc caccgtcgtc cgggccttcg ccaagcacct cgccccggcg gccgaggggc
     2641 tcggcatgga gaaggtcgcc atccaggcgc gcatccccga cccggcggtc cccggcggcc
     2701 tcaaggaggt cgtcttctcc gtctcgcagc cgggccggac cgggatcacg gtctccgtca
     2761 cgccgcccag ccgcaagccg ctccggaccc tctccgagta cgagcagaag gtcgtccgcc
     2821 tccgccagcg cgggctgact tacccttacg agatcctccg gatgctcacg cctccgaagg
     2881 acgacgccca cgccgacttc ccgccgggcg acttcgtcga gcacgacctc gacgaggagg
     2941 gcgccctcgt cccggtgagc cggccgcccg ggaagaaccg ggccaacatc gtcgccggcc
     3001 tcctgacgag ctacacggcg aaggtcccgg aggggatgac gcgcgtcgtc ctcctcggcg
     3061 acccgatggg ggcgatgggg tccctcgccg agccggagtg ccgccgcatc ctcgccggcc
     3121 tcgaccttgc cgagaagatg ggcgttcccc tcgagtggta cgccctctcg gccggcgcga
     3181 agatcgccat ggactcggga accgagaaca tggactggat ctcccgtgtc ctccgccgcc
     3241 tcatcacgtt cacgcaggcg gggggcgagg tgaacgtcgt cgtctgcggc atcaacgtcg
     3301 gcgcccagcc gtactggaac gccgaggcga cgatgctcat gcacacgcgc ggcatcctcg
     3361 tcatgacgaa ggacggcgcg atggtcctca ccggcaagac ggcgctcgac tactccggct
     3421 ccgtctcggc cgaggacaac ctcggcatcg gcggctacga gcgcgtcatg ggaccgaacg
     3481 gccaggccca gtactgggcc cgcgacatcc ccgacgcctg ccgcatcctc ctccgccact
     3541 acgaccacac ctacgtcgtc ccgggggagc gcttcccgcg gcgcgccgag acgtccgacc
     3601 cgatcgaccg cgacgtctgc ctctcgccgc acggcggcga cgagccgggc tcgttcgcgc
     3661 tcgtcggcga gatcttctcc gacgccacga accccggacg caagcgcccc ttcgagatcc
     3721 ggaaggtgat ggccgccgcg gcggaccagg accacgcgcc gctcgagcgc tgggcgggac
     3781 tgcgcgacgg cgagaacggc gtcgtctggg acgcccacat cgggggctgg cccgtctgcc
     3841 tcctcggctt cgagtcgcac cccgtcaagc ggatcgggtt cgtcgcgacg gacggtccgg
     3901 agaactggac ctcggggacg ctcttcccgc aggcctcgaa gaagatcgcc cgcgccgtca
     3961 acgccgcgag cggcaaccgg ccgctggtcg tcctcgcgaa cctctccggc ttcgacggct
     4021 cgcccgagtc gatgcggaag atccagctcg agtacggcgc cgagatcggg cgcgccgtca
     4081 cgaacttcaa ggggccgatc gtcttcaccg tcgtctcgcg ctaccacggc ggcgcgttcg
     4141 tcgtcttctc gaagaccctg aacgacaaca tggaggtcgc ggcgctcgag gggacctacg
     4201 cctccgtcat cggcggcgcg ccggcggcgg ccgtcgtctt cgcgcgcgag gtcgaccagc
     4261 ggacgaagag ggacccgcgc gtcgcggccc tcgagaggga aatggccgcg gcctccgacg
     4321 tcgagcggcg caagctccgc tcccggcacg ccgacctcgt cgccgcggtc cgctccgaga
     4381 agctgggcga ggtggccgac gagttcgacc ggacccactc ggtccagcgg gccctcgcgg
     4441 tcggctcgct cgaccggatc cttccggcga gggagctccg tccctatctc gtcgacgcca
     4501 tcgagcgcgg catggcgaag gagatgaaac ggatcggccg ctgaccgccg tccggtgggc
     4561 ggccgcgcgg tcgggagacc tgccgcacgg cgaggcgtgg ctcgcgcccg ccgagcgcct
     4621 cgcgctttcg cgcctcaccg tcccgaagcg gcgcggcgac tggctcctcg ggcgcttcgc
     4681 cgccaagaag gtcctcctcc tcgcgggtgt cgtcgcccgc gaggaggagg cggtcatcct
     4741 ggcggccacc tccggggcgc ccgaggcgca cgtcggcggt cgccccgccc ccttcgttct
     4801 ctcgatcacg cacagccacg gccacgcggc cgcggcgatc gcccccgccg gggcgcgcct
     4861 cggcctcgac ctcgagcgga tcgaggcgcg cccttcctcg ttcctcgagg actggttcac
     4921 gccctccgag cgcgccttcg tcggggcctc gccggcgggg gaggcggcgc tccgggcgac
     4981 gctcgtctgg agcgcgaagg aggccgtgat gaaggccctc cgcgagggcc tccggatccc
     5041 cccgcgggac gtcgagaccg cgccggctcc gggcccggcc gacggcctct ggcggcgctt
     5101 cgccccgcgc ggcccggggt ccgccgagtg gagcggctgg tggcgcgcgg acgagggctt
     5161 cgtcctctcc ggcgtcgccg acccgccctc cggccctccc gaacggatcg ggtagactcc
     5221 cgagccgatg ggctttttcg gatcgaagtc gtcgccccgc accgaaggcg agcgccccgc
     5281 accgtctcac gattccccgc gccccgcgac cgcgcccgtc ccgacgggcc cgaggacccc
     5341 aatcgaggtg aagacagccg tgatcggacc caacatccgc atccaggggg agctttccgg
     5401 cgacgaggac ctcgtcgtcg aaggtcgcgt cgaggggaag atctccgtct ccaagggcct
     5461 ccgcatcggc ccgcaggccc aggtgaacgc cgaggtcaag gcgcaccacg tcgtcatcgc
     5521 gggccgcgtc gtcgggaacg tcgtcgccgc cgagaaggtg gagatcctcc cgtccgggat
     5581 cctcgagggg aacatccgtt ctccgaagat cgcgatcgcg gagggggccc agttcaaggg
     5641 gagcgtcgac atgagcggca aggccgctcc ggccacgccc gcctcgcccg ctccggcggg
     5701 cgacaagaag gacgggtcgg cgaagggctg acccgtctct cgcctcgatc cgccggcctc
     5761 ctcgcggtaa cctcccgcag ccgtccttcc cggataccgc tgcatggggg tgaccacgtg
     5821 acccgatcgg tccgcgtctc cgtcctcctc ctcgcgctcg cgtcgctcgc cgggtgcgcg
     5881 tcgtccgcgc gcccgcgggc caccgcgctc gccgccgcgg gaggcaccgc cgcgcgcgtg
     5941 gcgaccgagt cgatgcgcga gaccgaggcc tccgtcgacc ggtacgtcga ggcgcagctc
     6001 ctcctcgccc cgctcacggg ccgccccgag ccgggcgagg agacgctcct cacgctccgg
     6061 agagtccgta ccgttctggc ggcgcgctcc gcggcgatgg ccgagctggg cgccgcgtac
     6121 gcgggcctcg acgcgctcgc cgccttcgat tccgccggca cggtcgagaa gggcgtcgac
     6181 ggcctcgtcg gcgctgtgag cgcctaccgc gaggcgctcg gccagcccgc catcgctccc
     6241 gttccggcgt tcggcctctc gaagggcctc ggtgccttcg ccggcgcccg gcaggccgcg
     6301 cgcctgaagg aggcgagcgc cgcgatccgg gagagcctcg agcgcttcgt cgcgctcctc
     6361 cggaaggagc aggaggtcca cgcctcgctc cgccgcgcgc tcgtcgaggg gcagggctcc
     6421 gcggcgcgcg ccttcgccgc cctcggcctg ggccggcccg gcgcgctcct cgccccgcac
     6481 gccgcggcct tcggcctcgc gtgggacgag aaggagtacg acggcgccct ctcgctcctc
     6541 cgcaaggagc cgtcgatcac cggccggccc ggagcgagcc gggaggacga cctgcgcgcg
     6601 gcgctcgacc gtgtcctcga gcatcgggtc gcgcgccgcg tcgacctcga ggccgccctc
     6661 gtcgacgaga cgctcgccgg cctcgaggcg ctcgttctcg cgcaccgttc cttcgaggcg
     6721 aagcgggaga ccgacctcgc cgccgtcctc gagcacgtcg cggccgtccg cgcgctcctc
     6781 gacgagtacc gcgcagcggc ggggaagtag gggaggaatc gatggcccgg acgaagtcga
     6841 accacgagcg gctcctcgcg accgtcgagg cgacctatcg cctcgccgag cggagcttcg
     6901 acgccctcca cgcggcggcg aagggtgccg aggagaggga gcgggtccgc gagctctacg
     6961 gtgccgcgcg cgacgcctac tggaaggccg tcctcgcctg cctggaagac cagagcggcg
     7021 tcgtggccgc catccacgac gagctgaagg cgaagaaccg cgagctgaag cgctccctcg
     7081 agacgctccg cgacctcgcc tcgaccctcg cgctcctccg cgacgccgtc cgcctcgccg
     7141 cggcgctcgt cacgctcgcg gcggcgtagg cgggcgggcc cgcgtctcca tcggacctgc
     7201 gcgacgaccg tcgtcgccgg cccgcacctc gcgaccagct ccggcgggac cggcccgggc
     7261 gcgaatcggt acgcgtgctc gccgaggtcc agcgagcagc ggcccggtcg tgcgacagcg
     7321 tgttcgcctt ctcgagcctc tccgcaaggc gcttcggatg ccgcgcgtcg gactcttgat
     7381 cgccgacggc gttggcctcg ggaaggcgat cgaggcgggc ctcggcccgc tccgcgacgg
     7441 gctgggcgac cacgagccgg gctcgccccg agatttgcta ctgcaagacg agttccggcc
     7501 gcgggcggcc ccgggccctc gtccaggttc gcgagacgct cgccgaccct gccacgcgca
     7561 cccgcgagac ctcggcgctg gaaacggcca gggccgggaa ggggctccgc tccgggacga
     7621 tcgtcacgaa agacgaggcg gagcggatcg cggcggacga cggcccgatc gaagccgtcc
     7681 cggcctggcg gttcctgctc gaccggccgg actcggggga agaggggtga ggaggagggg
     7741 ctcttcgtcc ggcgtcggga tacgggaatg gcaggtcagt gcgtgaacgg tgaaatgccg
     7801 tcgaggtggc agatgaacgg gatgacggcg catatcgatt cgtctggcag attggtggtg
     7861 cctgccgccc tgcggaagga gctgagggcc ccgcccggcg aggccgtgac cctacgtgtg
     7921 acggacggag agctgcgggt cgtcgctcgg agcgaagccg tcaggctcgc ccaggagaag
     7981 ctccgcaggt acgtgcagcc gggtcgccgg ctcgcggacg agctgatcgc ggaacgccgt
     8041 gcggagtgga agcgtgggtc gagtcgccct cgacgcctcc gcgatcctcg tcctcctcaa
     8101 cgacgagccc ggggcggccg ccgtggtcga agccatcgag gacgcggttg tcagcgccgt
     8161 caatctctcc gaggtcgtcg ggaagctggt cgagacggga atgccccgcc aggtcgccga
     8221 ggacgcactc ggcggaactg ggctggaagc ctgtcccttc gacgaagaag ccgcgtggaa
     8281 tgcgggtgag cttcgcacgg gcacgaggaa ggctggcctg tcgctcggcg atcgcgcctg
     8341 cctcgccctc gccaaggagc tcgatctggc cgccctcacg gtcgactcgg cctggaagaa
     8401 gaacgcggcc ggggtggagg tcaggctggt gagatgaggg gctggctcgc ggggtccgca
     8461 gctgcgctct ccgcgcgcct ggcgcccggg agccgcgatc gcgaccggaa tggccgtcct
     8521 atcggcggac gacgaacact gctgtggcac aggcatctcg atccccagcc tcctgatggc
     8581 gaggcacatc gtcgaccgcc ttagaattcc aagccgaagg agcggcccga aatgtcagct
     8641 tctcgcgcac tcgtccttcg cctcgtcgcg atcggcctcg ccgccggcgc accggcgctc
     8701 gcgacggcgg ccggaagctc cggcggagag ccgccgcgac accacttcgg cgatgtcgcg
     8761 cggccttccg tcgcgctgac cttcgagaag cccgagacgg ccggggcgcc gtgccacctc
     8821 cggctcgagc tgctcgcggg caaccgcgtc gaggtccgcg cggcgacgcc cgctccgacc
     8881 cgcgcgacct gccgacggac ggaagacggc tgggaggtct ccggcatgac ccgcctggag
     8941 gggaaggagc tcgccttccg cgcgagcgtc cgccccgacg ggagcgccct cgagtggacg
     9001 tcgccccgct cggtgacgtt gcgcgacggg agcgtccacg ctctcgccgg ccgctacccg
     9061 cggctcggcg aggaggcgcg ggccgccgcc gcccgcgcgg cgttcggggc cgtcgaccgg
     9121 gagctgaacg ccgtgtaccg gcgcctcgtg gcggctcggc ccccggaatc cgtcgccgcg
     9181 ctgcgcgccg accagcggcg ctggctgaaa taccgcgatt tcacgctcgt cgacggcgac
     9241 gacgccgcgg ccgtcgggcc ggggacggcg acgcacctgc gccagcaggc gctccggacg
     9301 ctcgagcggg tcctttacct gggcgcgctc ctcgacccgc cgcgcaccgc cctcggagag
     9361 acgctctact ccgacggccg gggcgggacg atcgccgtcc gtcgactcgg agacgcgctg
     9421 accttcgcgg tgcgcgccga cctccccggc ttcgacctcc cgaaggtccc ccgcgacgtc
     9481 ccgctccgcc tcggcggcgt cgcgcgcccc tcgggcccat ccgcgtggga ggccctgccc
     9541 gaggcgatga cggcctcgga cgggacctgg gcccccgagc ggctgcggct ctcgctcggc
     9601 gcggacggcc ggctccgcgt cgaggccgcc ggaggcgaga ccggccccct ggcgcgcgtc
     9661 ctgacgggcg acgacgcgcc gctgcggcgc ctcgcgcccg ccgactcccc gctccgcgcg
     9721 cttgtcgcgg acctgcccgc gcaggccttc gacgacacca cggacggcct cgagggagcc
     9781 gagcggcagg ccctgctgac cacgggtgcg ggcggggcgt tcacgatcgg ggccgagtcg
     9841 cccgaccggc tcttcctccg cttcccgggc ggccacgtca aggtcgccct cttcgcgggc
     9901 gccgacggcg gcgcggttct cgccgtcgag caggtgaacg ggcggaacct ctccgtgcag
     9961 ctctggcggc aggccgcgtg gggcgagccg ttcgtcgcgt acccggaggg gctcgccgcc
    10021 ctcggccgcg gcgtcctgca cctcgacgac tccgggacgc tccggcagac gcgctccggg
    10081 agcgggagcg gccgcgcctc ggacgacacc cgggtcctcg cgtgggacgg gttcggcttc
    10141 gcggccgagc gagcggacgc agcggcgccg gcggggaagt agccggccgg acgccggctc
    10201 cgctgcgggg tcgatcgtcg tcctcggaat cggcagaagc ggacccggtc gggttcggcg
    10261 gacggtcagg tgcgcggcgc cgggtgagcc cgagtcgtca gtcgtgaccc cagggcgccg
    10321 gcggaggggc gagcggcttc gagaggtcga actccacttg gaagaggcgt ccgccggggc
    10381 gcgagagctc gtagagaaag cgcttgcccg gctcgacctc catggcccag acgttcgtga
    10441 ccgaggccgt cagcccttcg cggcggaacg ccgcgaccga gtcggcgtcc actgggaact
    10501 cctgacggac ggcggtgccc gcggcggccg tgtcgccccc gtacatcgtc gtggcgtcgg
    10561 aggtgccgtc ctcgtgcctg tgatcgtgct tcaggcgcag gccgtcgggc gtgcgcgtca
    10621 gcacccacgt gcgggagcgg tcttctccga cgtggaaggg gatcctcacc tcgcccgcag
    10681 tgcaccgtcg gacgtgcatc acgagccgct taccggcgaa cgcgcttccc ggagcggccg
    10741 gctcgtccgc cacgacgcgg ccctcgaagg cctggccgca gagcccggcg agccgggaga
    10801 ggaactcgtc ggccggcgcg ggcgagccag gagccgcctt ccgcggggcg tcggaggccg
    10861 ccggttccgg cgactgagcg tcggccgagg aaggcgctgc cgccatgaag gcagcggaaa
    10921 ggacgaggac ggtcgagagt gggcggcgag ctcgcatcgt gttcgatccc ttcggctccg
    10981 gagaatagcg gggacggcag gcgaccggtc ccgagcgagc cggagtgcgc gagcctcgcc
    11041 gcacctcgcc gcacctcgcc gcagatcgcc gcacgccccg gcggcttcag gccacccgct
    11101 ccttcgcgcg gagtacgagc accgtgccgc cgacgacgag gagcatcccg gcgacgggcg
    11161 ccgccgacca gcggtagccc tcgaacgcgg tcgaggcgag catcgcgatg agcgggatga
    11221 cggcggccgt gtagcccgac cgccccgcgc cgatcctctt caggagcgtg aggaacgcca
    11281 tgaacgcgac gaccgacccg aagagcgcga ggtaggcgag cgagaggacg tacccgggcc
    11341 tcgcgtcgaa cgtgaacggg atgccgctca ccgcgcacca cgccgccacg gcgagggagg
    11401 cgtagcccat cgcgaaggcg atcgacgggg cgacggtcag cccggtcgag aagaggcgct
    11461 gcgagtagag gttcccggcc gaggcgaaga gcgtcgcgac gagggcgaga acgagcccgc
    11521 gaagctgcgt cgtgccggcg tgaaggctcg tcacctccgg ccagaagagg agcgcgacgc
    11581 ccgccacgcc gaggacggcg cccagggcga cggccggcgg cgccggagtc ccgaagaaga
    11641 cgcgggcccc gatcaggttc cagaagacga ggaacgagaa gatcacggcg acgagtcccg
    11701 acgcgaggtg ctgctcggcg cggtagacga ggacgtagtt caggccgaag aggaggaagc
    11761 cgaggagcgc gagccgggca tggtcgcgcg ccgagaaccg gagcgggatg ccgcgcagga
    11821 ggcaccacgc gacgaggagg agcgaggcca gcgcgaaccg ccacgccacc gagacctcgg
    11881 gcgccacgac cccgagctgg aacttgatgg cgagccacgt cgttccccag atgaacgaag
    11941 agaggaagaa caggaagacg tcggaagagc caccgggctt cggcgacgcc gcgggggagt
    12001 gattcatccg gcggagtgtg cacccggcgg cgcccgcgga acccgcggag aggtcagacc
    12061 cccgccatgt cgaatccctc gccggccacc cccatcgacg agcggcctca cgtcctaagg
    12121 cgcgagccag acctcgagct tcaggggctg gttgaagtcg atggtcgggt acccggtgcc
    12181 gcagctctgc tcgaagacga tgtgcggccg ggcggcttcg ccggcgaccg cgaagacgag
    12241 ccgtcccgag acggagcgca cagggtcgag cggcaggttg atccagtggg acttgaagct
    12301 ccaggtcccg tcggccttga accagaggag cgtcttccgg tcggcggaga gctccccctg
    12361 gtaggtggcg acgttctgga agttgacctc gaaacggttt ccggcccaga cgagcggaaa
    12421 gatgaagctc gactcaccgt tcgacttgac gctgcatcgg cccgaggggc agcccgtcgg
    12481 cggggcatcc gggggacaca tgtagatgat cgagtcaccc agcgagatcc gtgtgaggtc
    12541 gcctccgagc gtcaccttcg cgacggcgtg gtcgatggag acgttcacga cgagcaccgt
    12601 cagcgtcgcc gtctcgacgt cgatcggaac ctcgaccggc ttctggatgt ccgccacctc
    12661 gaggtccagg cccttctgga ggacgccgtc cttctcgggg aagacgaaga cgcgcacccc
    12721 gcccttcccg aaggtcgttc ccgtgacggc cacgttcgtc gtgaacttct tcgcggtccg
    12781 ctccgcgtcc gagaccgtca ccctcaccgc ccgcgccgag agcgggtcga tgtcggccag
    12841 ctcggcccgt ggcgtcgtgg cggggtccca ggccagggcg gcggggaaga gcgtcgggtc
    12901 cagggagttc ttcttcaggc tgcggtccgc ggcccggagg agcgccggct cgtcgtgccg
    12961 gtaggcccgg tccaggacga actccgtgta gaggtccggc agcgagaggc cgaaggtgct
    13021 ctggtagtgg aagtccatcg ccttccggta gagcgtgtag tactcgtcgg ccggcttccc
    13081 gaactgcccc tccgtctggt ccgagaggtg ctggaagagc cagcgcaggt cccgcagcac
    13141 gccgccgttc cagcgcttcg ccacgtacgc gaagaaatcc tgccgtgcgt aggcggccac
    13201 tccggtgggc cagtggaagt cctgctcgag tctctgggga ggttccggcg acctcaccgt
    13261 cacctcgccc ccgtagagcc cgttgaggtt cgactggaac atctggccga cgagcgtcgc
    13321 cgtcccctcc gtcatcgaca tcgccgagtg catccacgtc ccgcccgact ggaaccaccg
    13381 gtcccggaag tcgtaccccg cctggacggc gtggaagagc tcgtggatcg agacgttctc
    13441 cagcgagaag ccgctgggct gaatgctcgt gttccagtcg tcccagttga agtacatctg
    13501 cccgaggctc tgcatctgcg cgtccgtgta gccggccatc gaaaacgccg cgtcgtcgag
    13561 cgtctgcgag aacgacgtca ccgggtcgtt cgggccggtc ccctggacca ggtgcacggc
    13621 ccgcgccttc gggttccagc ggccgtccac ccagagccgc ggcgagcgga actcggctcc
    13681 gcgcaggtgg tcgcacgctt tcttcatcgc cgcgggcacc ccggtcttga accccgccga
    13741 cgcggaggcc gccgcgtcga agacccggaa ggcgcacgtc tgccagtcgg tcggcgtcac
    13801 ccacccgagg gacgagatgc cccccgagcc cagcggcgtc gtcgaggtga tcgccagcga
    13861 gggccgcgcg acgaccccca tcgtccagcc gttccacacc ttcatcagct cggccgtgaa
    13921 cgtctttctt cccgcgtcat aggcgcccgc caccgggtag acacggccgg tgctgacggc
    13981 caccttcagc agcagcttct cggggtcggc gacggtcccg gagaccggga gcgtcaccgt
    14041 gaacgacccg ttcccgatcg ccacgcccgt cccgctcacg ttcgtcttca cgacggtgga
    14101 gaccagcgtc tcgcccggct tcgccagcgt gctcccgtcg ccggacgtga gggtgacggt
    14161 gcccccgctc gtggtcgcgc cgggaggcag cgtgaccgtc gtcccgccgg ggcccgtgat
    14221 cacgcctgtt cccggcgtcg ccgtgggagt cggcgtcggc gttcccggca gcgccgtcgc
    14281 cgccagccgc gtcgccggga cgaagaccgg gtcccccgtc aggtggttgt cgatcaccgt
    14341 cgcgaacgcg aagaacctcg cccccgccgt caccggacgg acgacggcgt agccctcgtc
    14401 gatcgccgag ccccacgccc cgaagaccgc gttgatctgc ccgaacccgt agggggccag
    14461 ccgcgtccgc tcgtcctgga tcgttcccag gagcgtgccg tccgagcggt agaggtcgat
    14521 ccgcagctcg atcgccgtcc ccgtcgtgct gacgtagccg atgttcgacc ggaacccgtt
    14581 cagcgttccc gagtcccggt gcgagagctg gatgatccgt ccttcctggc ccgccccgat
    14641 cgcctcgctc tccgcgaggc ccggcacgta ctgcccgaac gacgccccca ccggaaggcc
    14701 gacggtgttc gccccgatca ggttgtaggt ccggctgttg gccaggacgc ttcccgacgt
    14761 cgtcgcgatc cgaagcgccg cagcccccgt gtacccgaac acgctcgaca gcacgtccgc
    14821 gtaccgcacc gactgctgcg ggccgagcga gaagctcttc accggcgccc cgctcccgtt
    14881 gtcctgcccc tgccgcagga gggtgaccgt gtagctcgcc gccgtcgccc ccgggttgtg
    14941 cacctccagg tccgttcgcc agctcgcccc gttgaccccg ctggcgtgcg cgctcgccgg
    15001 aacgtagatc ggcccgctcc ccgccagcgc ggcctgccgg gggagcgctt cggccagcgg
    15061 ctccggcggg cccgcgccca gctcgtcctc cgcgccgctc ccgtccggcg ccgcgccctc
    15121 gtacagcagc ggcgccccca tccacgtcgc cgtcgtcacg cagacgttgt cggccccgtt
    15181 cgtctccgag atcgtgtcgt ccgggtcgat cgtcagccgc agctcgtgcg tccccgccgc
    15241 caccgggccg gtgaactccc acgtcagcac gatcccgtac ccgggctcca gccccgagtc
    15301 gttcgcccag ttcgcgtacc ccgccaccgc cccgtcgatc gccagagaaa gccgcaccgg
    15361 ccccgtcagt cgcaccgtcc ccgagttcgc caggaagatc tggacgaacg cgctcgaccc
    15421 gctcaccggc gccggcgagc agcacgccga gctcgtgcag cagcccgagg ccgagtggac
    15481 gacgatgcac gccggccacg tcgccggcct gtagaagtag aggttcgggt aaccgcccgc
    15541 gggaggcgtc ggagtcgccg tcggagtcct cgtcggcgtt ggcgccggcg ccgcgcccgt
    15601 caggacggtc gccgggacga agaccgggtc ccccgagttg tggttgtcga tgaccgtcgc
    15661 gaaggcgaaa agcttccccc ccggcgtcgt cgtcttcacc accgcgtacc cgtccgccac
    15721 cgttccccac tcgcccagca cctcgttcag ctgccggaac ccgagcgccg gcaggcgcgt
    15781 ctcggctccc tgcctccttc cgaggaaggc tccgttggcg cggtaaaggt ccaccgtcac
    15841 gtcgatcggg ctcgccgtcg cgttcacgat cccgaggttc gtccggaacc cgttcagcgt
    15901 cgccgagggc tgctgcgtca ggtggatcag ccgcccctcc tgcccgtagg cgatcgcttc
    15961 cgattcctcg atgcccggca cgaactggct gaacgaggct ccctgcggaa gcccccaggg
    16021 gttcgcgccg agcaggttgt acgtccggct cgtcaccacg agcgaacccg acgtcacgcc
    16081 gatccgcagc gccgccgccc cgtcgaaggc gaactccgtc ttcaggacgt ccgtgaacct
    16141 ccgcgactgg cgcgccggga gcgtgtacga cttcgtcgcc gaggaggcgt tgtccgcgtc
    16201 ccgcaccagg agcttgaccg cgaacgccgc cgccgtcgtc cccgggttgt gcacctccag
    16261 gtcggtccgc cagctggccc cgtttgctcc gctcgcgtgt gctgccgccg gaacgaacac
    16321 cgtcgccggg gccgtcgccg acaacgcacc gccggtcgcc gccccgcccg gctccccgaa
    16381 cagacccccg agcccgcccg gcagcgacag ggagaggacc gtgactgcgg cgatcaggag
    16441 aggaaggggc ttgatacgac gcgaggaccc gggccgggtt tcgctcatct tccgctcccc
    16501 ccgggacgaa cgcccgaaag gtcccgggaa acgcctcatt gtcagccctc ggggcgagtt
    16561 cacctagcct tcttcgtcgc cgcccgggcc gcgcgcgaga cgttcgcgac gcgggcgagg
    16621 gaggcgctcg tcgcgcgcgg gcggcccgac acccccggcg cccgcggcga ggtaatgtcc
    16681 ccgccatgtc ggacctttcg ccggccaccc ccatcgacgc ccgctcgcgc gacatcggcg
    16741 gcttcaccgt ccgccgcctc ctcccgtggc gggcccggcg catggtcggc ccgttcgtct
    16801 acttcgacca catgggcccc gtggcgctcg aggccgggcg cggcgtcgac gtcctcccgc
    16861 acccgcacat cggcctcgcc accgtgacct acctctacga cggggcgctc ctccatcgcg
    16921 acagcctcgg cagcgagcag gtcatccgcc cgggcgacgt caactggatg accgcggggc
    16981 gcggcatcgt ccactccgag cggacgccgc ccgcggagcg cgcctccggg ccgcgccttc
    17041 acggccttca gctctgggtc gcgctcccga cggccgacga ggagatccag ccctccttcc
    17101 gccaccaccc gggcgcgacg ctccccgaga cggaacgcgg cggcgtgagg ctgcgcgtga
    17161 tggccggctc cgcgtacgga gccacctctc ccgtcgaggt ccgctcgccg ctcttctacg
    17221 tcgacgtcgc catgccggcc ggctccgagc tccctcttcc ggacgagcac gccgagaggg
    17281 ccgtctacgt cgtcgagggc tcgctccgcg ccgccggcgc gaccgccggg cccgggcgga
    17341 tgctcgtctt cgcggaaggc gccgacgtca ccctgaaggc ggaggcggcg acgcgcgccg
    17401 tcctcgtcgg cggcgctccg ctcgacggcc cgaggcacat cgactggaac ttcgtcggaa
    17461 gctcgaagga gcggatcgag aaggcgaagg cggactggag agaaggccgc ttcccgaacg
    17521 tgccgggcga cgagacggag ctcgtcccgc tgccggggtg acgaggccgc acagggggac
    17581 gtagccaccg agaccgcttc ggccgcggac cggagttcac cgacgtcgag gacgggcggg
    17641 cccgcgacgc cctcagagcc agtccgtcag gcccgcgtcc gagcccggcg tcgcgcccgg
    17701 tccgtcgccc gtgccgcccg gcgcgggagg cggggccgcg tgcgcgccat ggcgctggac
    17761 gacgacctcc ggggcgctgg ctgcgaggcg gtggaggagc ggcagctcgt cgccctcgac
    17821 cgcggagagg tagacccacg aggtcgcgcg ggtgatgccg acgaagagga ggcggaggag
    17881 ggacgaggcg ccccgggcga ggacgtcgga ggatgcgagg cgggggagga ggacggagtc
    17941 gaaggtgagg cccttggcgc tgtggtacgg catcaccttg acgaggtcgg aggcgaagtc
    18001 gagcggcggg aaggggcccg ggcgcgtgcc ggggcggtgc gggacttcga cggggatgcc
    18061 gaggcgggag aggccgcagg cgagggaggc gacgaggcgg cggcccggga ggaggacggc
    18121 gacgcgctcg cctcggagga gccgcgcgcg ggtgacgtcg gcgaggcgga cgatctcgtc
    18181 cgcgtggccg ccggcgaggt agaggagcgg cttctcgcgc tccaccggcc cggcgagcgc
    18241 gttccggagg aaggcctggc gcgcgtcgcc ctcggggagg aggcgggagg cgaggcgcga
    18301 gacgcagggc gagcagcgcc aggcgtcgag gagggcgacg gagcgcgagg ggatgccgag
    18361 ggcggcgagg atctcctcct cgcgggctcc gccgtcatag atccgctggc gcgggtcgag
    18421 cgcgacggtg aggtggcggg agacggcggc gagggtctcg aaggcgtgcg cgtcgaggtc
    18481 ctgcccctcg tcgacgagga cggcgtcgta gagcggaggg cggtcggggt ggcggcggac
    18541 gtcgcggagg acgccgtcgc ggatggcgtc gaagcggggg cggccgcgcc gctcgtccca
    18601 cggcttctcg gccgagacga accgctcgta gtgcccggcg caccagtcgt cgaaggtgcg
    18661 gacggcgctc tcggggatgc cgagctcggc gagggcggag cggaggaacg cggtgagggc
    18721 gttcgtgtag acgaggacga ggagccggcc ctcggggatg cggagggtgt cggcgaggcg
    18781 gcgggcgcgg tggaggagga cgagcgtctt gcccgagccg gggccgccgg agaggacgcg
    18841 gtgggcgtcg gccgggagct cgacggcccg gagctggtcg ggcgtcaggt cgcggagggg
    18901 gacgagccag cccgccatct ctgatccgct cagttcgtct gcgcgaaggc ggcgaggacg
    18961 gggaggaggc cgtcgacgaa cggcaggccg ggcttcgcgg cgcggcgccg cttctcttcg
    19021 gcttcggccg cggcgaggcg gggcgcgtcg cccgcgagcc ggcaggcggc gaggtaccag
    19081 gcgagcgcga agtcgccctg cgtggggagc ggcgcgaagg aggcgaaggc ccgggcgagg
    19141 tcggcgtcgc gggcgggaac gtcgccgagg gagtcgtgga cgagggcgcg gaggacgagg
    19201 tacgccgcgt ccggttctcg gcggatcgcc tcggtcgccg tggagagcgc ctccgccagg
    19261 agcccccggc gcctgcgggc gagggcgagg ttgaagaggg gctcgctgcc ggtcgggtcg
    19321 gcctcggcgc tctcgaggta gaagcgctcc tcgcgcccgt cgtcgcccag ctcgcccgcg
    19381 aggaggccca tgcggaagag gaggtcggcg tcgggccggc ccttcgcgcg gaggagggcg
    19441 cggtagacgg agagcgcctt ctcgcgctgg ccgaggtcgg catagaggcg ggccgcctcc
    19501 tccgtggccg cggcggcctt gctcgcggag agggcgccgg tgcggaggct ctcctcgatc
    19561 tcgaggaggc gctcgcgctt cggctgggag ttgacgacgt tcgtgagcgg gttctcgagc
    19621 cgcagctcgt gcccgccggc gccgggacgc gcggggaggt cgacgcggag gtggaggagc
    19681 tggttcgcgt cgacgcggta gcggaggcgc aggggctcgc cctggcggac ggggggaggg
    19741 aagaggcaga gggcgttcat cacggggccg ccggcgccgt cgcgcagctc gacgcggagc
    19801 gggagagctt cgcgctcgcg cgattcgggg acggcgagct cggtgcaggc ggcgaagtcg
    19861 ccggaggcgg ggaaggggag gggcgtgccg cgcgcgacga gctcgacgag gcccgaggcc
    19921 gtgcggacgg agagggcgcc gccggtgacg ggcgtcagga gaccgcgccc cgaggtggcg
    19981 agcgaaaggg cgtggagagc ggcgccgcgg gccacggacg actggaccgc ctcgggcgtt
    20041 tcctcgcgga ggacgcgggc ggaggggaag cggcgcgcga cggcgtcgac gacctggggg
    20101 atgaggctgc tcccgccgac gaggagcgcg ccgtcgatct ccccggcgga gaggccgccg
    20161 cgggagagag cgtcctcgat gggggcgaag atcgagcagg tggtgcggta ctcgtctccc
    20221 ttcgcgtaga ggagctcttc gtcgaggaac ggaaagagga cgccgtcgag ctggcgggtg
    20281 gtgaggcggg ggctcgtgag gcggaggtcg cggccgtcgg ggaggcggca ggggtacgag
    20341 ccggggaggg tcttcgcgac ctcgttcggg tcggcggcct cccagcggcc gaggccgcgc
    20401 aggcgccgga tctcggagca gaggccgacc ttgagcgcct cggcgacgcc gaggagggcc
    20461 gggacgacga cccgggcctt ctcctcgtag tcgaggtcgt gccggccgag gccgttctct
    20521 tcgaggaggc gcgggatgag gagctggtgg acgagggccg cgtcgacgtc tccgccgccg
    20581 aggcggtggt agcgcgagac ggcgagcgtg gcggcgcgga gcggctcgcc cggggcggcg
    20641 agggtgacgc ggaagacggc ggtgtcgcag gtgccgccgc cgaagtcgaa gacgaggagg
    20701 tgcttcgtct cgccggcgcg gccgagggcg tctaggccgt tgcggtcggc gtggtcgagg
    20761 aaggcggcga tcggctcgtc gaggaggtcg ccctcggaga cggggaggcc ggcggcgcgg
    20821 gcggcggcga gcgtgtcggc gcgctgggcg gcctggaacg acgcggggac cgtgacgacg
    20881 acgcggtcgg ggggcggcgc gcctttgcgc gcggcggcgg cggcgaggaa gcggaggacg
    20941 tgggcgccga tctcggaggc gttccgacac ccctcggggg cgcggtggta cgtcttccgg
    21001 acgccgatgt cgttcttcgt gtcccagaag aggtcgcggc tcggcgagag gccgagctcg
    21061 ccggcgcggg cgcgaaggcg cttcgccccc tcgcccaccc aggtcttccc gcggtggatc
    21121 gcgacgacgg acgggacgag gacgtggacg tactcgccct cgggcgtctc ctgcttcacc
    21181 tcgagcgtcg tgagcgtccg gccgccgggc ccggcgaccg cttcggtgac ggtggagttg
    21241 gtggtgccga ggtcgattcc gaggaccctc acgtggcgtg cctcatcgga agtccctcca
    21301 ccgggaagga tcggacccga gggtggacag ttggtgtccg cgggagggga atctgctact
    21361 gcgccgtcac tttcggcgcc tcgcgaggcg cacctcgagg aaggcgatgt cctcggcgtc
    21421 tcctggtgca gaggggttct ccgacccccc tgtacccgac cgctccggtg ccaggcgtga
    21481 aatcgtgtat tgtggagaca atacacgact tcacgcctgg cacccaggtc gggcacccag
    21541 gtcgggcacc caggtcgggc acccaggtcg ggcacctggg tcgcggaacc gaggtcgcgc
    21601 ggacttggca ccaggacgga cttcgtcccg ctgcctggga gacgaggccg cctcctcgat
    21661 cggctgggga ggagctcggc tccgcaggga ggcgaggctg ggtgtgtcgg gccggatggt
    21721 cggccgtgga ctcgggggtc aggctcgggc gggtcgctcc ggcgtatatg tcagacgtat
    21781 actccacctg tggcaacgcc cgatctcctc gacggctgca ccggcttcga ctgggacgaa
    21841 gccaacgtcg agaggaaccg gatcagtcac cgcgtgacgt tctgggaggc tgaggaggtc
    21901 ttcttcaacg atcctctcct ccttcggccc gacgaggctc attcaaggct cgagcgccgt
    21961 ttcatggctc tcggctccac cgacgccggc cgtctgctct tcatctcctt caccgtccgg
    22021 cgccatctca tccgggtcat ctccgcccgc gacatgacgc gaagggaggc cagaacccat
    22081 gcccaagtca aagcgtgagc cggtcccttc cttcgcctcc gaagatcagg agcgggcctt
    22141 ctgggcctct catgactcca ccgacttcgt cgactggacg aaggctgagc ccaggtcgtt
    22201 tccgaacctc aagcccacac tccgcaccat ttcccttcgg cttccggagg ccatgatcgg
    22261 tcagctcaag gtgctcgcga acaagcgcga catcccatac cagtcgctcc tgaagcagtt
    22321 cctggctgag cgacttcggc aggagctgtc aaaggcagcc ggagcctgac ccggcgtgta
    22381 gcggacttca gtgcgctgcg ctcgctcgcc cctgacgctg cggatcaacc gcccgaggaa
    22441 cgcctcactc ctcttcgtgt tcgccgtggc gcttcgttcg gcccggtcct cgaggctcgc
    22501 ggcgaagggc agagtccttc ccgcgatcgt cctcctcgca tcgctgaccg ctgccggctg
    22561 cggcacgcgt gttcacgagc acgtcctgga ggtggtcgtc cgcgatccct ccggacggct
    22621 cggcacgccg ccccacgagg tcggggtctt cgactggagg atggggcgaa gcgcggagtg
    22681 ggcgcggaag accgccggga cggcgggtgt ccggccgtat cggacgacct tctcgacggt
    22741 cgacacggtt tcgatcggag tcgcgcggcc ggagaaggtc gagctcgccc tctggcttcc
    22801 tgggctcgaa cgcgagggct tctacccgct tcgggtggag ccgaaggcgc ggcccgcagg
    22861 gaacgccacg ctccaccccg tccgtttcga tgagtcgccg gcggacggca ccgcacgatg
    22921 gctcgccgtt cgatggacga cggcgccgat ggagaggggc tggcgcctcg agctcgacct
    22981 cgaagtaccg gccgacgccg agaggggaag gacggctgag cgcggtgtcg gagcgacgag
    23041 cgcgccgatt ccgcttgacc cctcccgtcc gcggcgatag gctcttggtt gcggatgatc
    23101 cgcaaatagg aatgaaacgc agagcggggc ccgaccggat cgagcagcgg ctcgagcgcc
    23161 tccggacggc ggcgcgaggg gcggggctga agctgacgcc ccagcggctc gagatcttcc
    23221 gggagatcgc gggaagcgag gagcaccccg acgtggagac cgtcttccga gccgtgcagg
    23281 agcggatgcc gacggtctcg ctcgacacgg tctaccggac gctctgggcg ctccactacc
    23341 tcggcctcgt cacgacgctc gggccgcagc agaacggcgt gcggttcgac gcgaacctgg
    23401 agaagcacca ccacttctcg tgcgtgagct gcgggctcgt gcgcgacttc gagagcccga
    23461 agctcgacga cctcccgctg ccggccgacc tgaagagcct cggcagcgtc ctgaacgcgc
    23521 acgtcgaggt gcgtggcctc tgcacacggt gcgaggccga gaagaagacc gactcctaga
    23581 ccctgacccg agcccggtcc cgagcaaccc gcctcaccta aggagaacga cgatgagcga
    23641 aagcggcaag tgccccttca ccggtgcctc cagccacccc gtggccggcc ggggcacgac
    23701 caaccgcgac tggtggcccg agcagctgaa cctcgggatc ctccaccagc acgaccccgc
    23761 gtcgagcccg atggaccccg gtttcaagta cgccgaggag ttcggcaagc tcgactacga
    23821 cgccctgaag aaggacctgt acgccctgat gaccgactcg caggagtggt ggcccgccga
    23881 ctggggccac tacggcggcc tcttcgtgag gatgtcgtgg cacagcgccg gaacgtaccg
    23941 ggtgtcggac ggccgcggcg gcggcggcac gggaaaccag cgcttcgcgc cggtggggag
    24001 ctggcccgac aacgggaacc tcgacaaggc gcgccgcctc ctctggccga tcaagcagaa
    24061 gtacggcaac cagatctcct gggccgacct cctcatcctc gccggcaact gcgcgctcga
    24121 gtcgatgggc ttccggacgt tcggcttcgc cggcggccgt gccgacatct ggcagccgga
    24181 ggaggagatc aactggggcc gggagaagac ctggctcgcc acgagcgaca tgcccgacag
    24241 ccgctactcc ggcgaccgcc agctcgcgaa cccgctcgcc gccgtgcaga tgggcctgat
    24301 ctacgtgaac cccgagggcc cggacggcaa ccccgacccg atcgcctcgg ggcgcgacgt
    24361 gcgcgagacg ttcgcgcgga tggcgatgaa cgacgaggag acggtcgcgc tcgtcgccgg
    24421 cgggcacacg ttcggcaagg cgcacggcgc gggcgacccg aagctcgtcg gaccggagcc
    24481 ggaggcggcc ccgctcgagc agcaggggct cggctggaag aacgcgctcg gcaccggcaa
    24541 gggggtccac acgacgacga gcggcatcga gggggcctgg aagccgaacc cgacgaagtg
    24601 ggacatgggc tacttcgaca tgctcttcgg ctacgagtgg gagctcgtga agagcccggc
    24661 cggcgcgtgg cagtggctcg cgaaggacgt gaagcccgag cacatgatcc ccgacgccca
    24721 cgacccgtcg aagaagcacc ggccgatgat gacgacggcc gacctggcgc tccgcttcga
    24781 cccggcctac gagaagatct cgcgccgctt ccacgcggac ccggcggcct tcgccgacgc
    24841 gttcgcccgc gcctggttca agctgaccca ccgcgacatg gggccgcgct cccgctacct
    24901 cggcccggag gtcccgtccg aggtgctgat ctggcaggac ccggtcccgc cggtcgacca
    24961 cccgctcgtc gacgcgaagg acgtcgccgc gctgaaggcg aaggtcctcg cgtcgggcct
    25021 cggcgtttcc gaggtcgtcg cgacggcctg ggcctcggcc tcgacgttcc gcggctccga
    25081 caagcgcggc ggcgccaacg gcgcccgcgt gcgcctcgcg ccgcagaagg actgggaggc
    25141 gaacgacccg gcgcagctcg cgaaggtgct cggcgtcctc gaggggatca agcgcgagtt
    25201 cgacggcgcc gcgaagggcg ggaagaaggt ctcgctcgcc gacctgatcg tcctcgcggg
    25261 ttgcgcggcg gtggaggcgg ccgcgaaggc ggccggctac gccgtggagg tgccgttcac
    25321 gccgggccgc accgatgcga cgcccgatca gaccgacgcc gactcgttcg ccgtcctcga
    25381 gccgatggcc gacggcttcc gcaactaccg gaaggccggc accgcgtcga tcgaggagca
    25441 cctcctcgtc gacaaggcgc agctcctccg gctcggcgcg ccggagatga cggtcctcgt
    25501 gggcggcctg cgcgtcctcg gggcgaacgc gggagggtcg aagcacggcg tcttcacgaa
    25561 gcgcccgggc gtcctgacga acgacttctt cgtgaacctc ctcgacatga cgacggtctg
    25621 gaagccgacg tccgacgcgc gggagacgtt cgaggggagc gaccggaaga cgggcgcggc
    25681 gcgctggacc gcgagccggg tcgacctcgt cttcggcgcg aactcgcagc tgcgcgccct
    25741 cgccgaggtc tacgcgcagg acgacgcgaa ggagaagttc gtccgcgact tcgtcgcggc
    25801 cttcgcgaag gtgatgaacc tcgaccgctt cgacctcgcc tgatcgaggt cccgagtccc
    25861 ccggcgggcc ggcgggcccg cccacgcccc ggttcggcgg tcggccagag gccggccgcc
    25921 ggaccggggc gcttctttcg tccctcgtgc ctggaccttc gggagatccg gtccgcgcag
    25981 ccgtcgtacc atcggcggct atgcgtcccc aggatcagtg gaacgaccgg agcacccctc
    26041 ggcgttcccc gcgcgagcgg ctcgcgtccg cggcgcttct cgccgcgctc ctcgtccccg
    26101 ggacgggcgg ggggcaggag ccggagaagc tgccgcgcgt gaggggctcc gtcgagctct
    26161 cgctcgtcaa cgtcgacgtg gcggtgacgg gaaaggacgg gcggccgatc gaagggctct
    26221 cggcgtccga cttcaccgtc gtccacggga aggcgccggt cgcgatctcg aacttccgcg
    26281 aggagcggct cgtgccgcgg gccgaggtcg cgacggctgc gcccgcgccc gccccggtcg
    26341 cggcccaggc cgcggcggcg atccccgagg cgcctcccgc ccgccggcac gtcatcctct
    26401 tcgtcgacca cctcgcgctg cccgacaagc gggagcgcga gcaggtcttc ggctcgctga
    26461 aggcgctcgt gaggaagacg gtgcggcccg gcgacggcgt gatggtcgtc tcctggcggc
    26521 gcggggtccg gacggcctgg ccctacaccg acgacgtggc tcagatcgag cgtgcgctcg
    26581 acctcgtggc cggggggacg atccggctgg accgcgacgc gcagaacgac ctcgaccagt
    26641 tcgcggcgac ggaggcgttt caggcctggg ccggcgagcc gcccgacacg tcgctctcgc
    26701 gggacctgag cgtggagcag gcgtacaacg agctgaaggg gaaggcgtcg gcggtgatgg
    26761 ggctcgtctc gacgatggcg gggctggagg ggaagaaggt cctcgtcctc gcctcgcgca
    26821 gcttctcccg ccggccgggc gtggagtact cggggcaccg cgtcgagacg ggacggctcc
    26881 tcgacgaggt ggccgcgcgc gcgaacgcgg cgggggtgac gatccacgcg ctctacgccg
    26941 cggcgtggca gcccgacctg ccgaacgtca cggactcgcg cttccagccg ccgggggccg
    27001 cgggctcgcg ctccgcgacc ggacggagcg acgcccgcct cgaggacctc gccgggctgg
    27061 agacgctctc gggccggacg ggcggcgtcc tcgtcgggac gacgatggag gcctcgctct
    27121 tcgccgataa ggtctcgtcc gacctcgtcc actggtactc gatcggatac cccgtccctt
    27181 ccggcgcgag cgcgtcggcg cgcatctcgg tccgcgtgaa ccggaagggg gcgaaggtga
    27241 ggacgcggcg cgaggtgatc gaccggacgc cggaccagcg gatggaggac cgggtcctcg
    27301 cgaacctctt ccgggccgac ctctcggggc ggctcccgat cggcctgtcg gtggagcccg
    27361 cgcgccgcga gaaggagcgt tacgtccgga cggcgacgct ccgcgtcccg gtgaaggacc
    27421 tcgtcctgct gccggaggcg gccgcgcggc gcggcgaggt gaaggtcttc tgggtcgtgg
    27481 ccgagccgaa cggcgagttc tccgacgtgc ggagcgagcg gcacgaggtg gcgtttcccc
    27541 cggaggagag tgcgcccggc ccggagacgg agctgcggct cgaggtcccg atcgagacga
    27601 gcggcccgga ggcgcggatc tcgctcggcg ttcgcgacga gatcggcggc gaggccggct
    27661 tcggcctgat tcgccccgcg gcccgctgac gggcggaggc gtccggaacc gtgccggcgc
    27721 caccggccgg aggcgcccgg ccgccgggcg cgggggcgcc tcgccgatct atcatcgcgg
    27781 ccgatcgaac gacggagagc cgggcttcgc gaggacgcgc gcgctcggtc ggaggacggg
    27841 ggagcaggac ccgccatggt atggtgcgga ggatgcgaag caaaacgaca gggcgggcgc
    27901 tgcgagcgcg ctgccgggcg gcgcgggtcg cggtgctcgt cttcgtcgcg acgtccgctc
    27961 cgcgcctcgc cgcgggcgag ggggcggaga gcgcgggcgg gatggagctc ccgtcgatcg
    28021 tggagacctg tccgctcttc cggagcgacg gaggagccga gggctccttc gggttcggcg
    28081 agggggcgtt cggaggcgag cagtcggcct ggaggacgga gccggtgagc ggcttctcac
    28141 tctgcccgcg cctcgaggcg acgcgcgtcg ccgagcggcc cgagctgcgt tgggcgccgt
    28201 ggacggcgcg ggacccgcga gagccgctcc gcggcgtgcg gaacgcgacg gcgccccatc
    28261 ggaagaggct cgtccgcagc acggtgatcc tgaccgggac ggcgatcgcg agcggggcga
    28321 tcctctcgct cctgccgaag gaggagacga actgggagga gggggccttc gaccacggcc
    28381 tctcgaacct cgggcgcgcg tggacggagc tgcccgtctg ggaccgcgac ggggccttcc
    28441 acgactggtt cgggcatccg tacgcgggcg ccttctacta caacatgatg cggtcgcagg
    28501 gggcgacggt cagccagtcg ttcgcgtact gcgtcgccca gtcggtcctc tgggagtacg
    28561 tcctcgaggc ggcggcggag cagccgagca tccaggacct cgtctcgacg cccatcatgg
    28621 gcgcggcgct cggggaggcg ctccaccgct ggtcgatctc gatcctgaag aaggggcggc
    28681 tgaatttcgg ggagaaggtg ctcgtcttcc tcctgaaccc gtcctacgtg atcaacaacg
    28741 gctaccgcgc cccggagtga ggcgcggcgg cggcggggcg ctgcccgctc agaacgcgaa
    28801 gaacgtcccg gcggagaggt ccacctggta ggtgccgttc gacgggatgg cgacttcgca
    28861 gctcgaccag cggcagaggt cccgccggga gctccccgag aagaagccga gggcccgcag
    28921 ctggacccgg aggccgaagc ctcgggtcgt catcaccttg acgccgccgg agagcgaggc
    28981 cgtgaaggag gtcgtgtagc cgccgccctg ggcttcgaag gaggtcgcgc cgacgccgcc
    29041 cgagacgaac gggcggacgc gcgccttcgg cgcgaagtcc cactgcacgc cggcgagccc
    29101 ggtttcgagg tccaggtcgt ggaggttgcc gtcgcccccg aggaacgcgt tctcggcgtc
    29161 gaggcgcgtc gcctgccgga ggaacgtccc ctcgaagatc aggttcgggc ggccgggcgc
    29221 gatccccgcc acgaagcccc acgaccacga cggccggacg gagaaccact gcccggtccc
    29281 gaagtccttc aggcgccctc cggagcgcgc gccgaggaac ggcgtcagct cgacgatccg
    29341 gacctcctgg gcgcggaggg gaggagccgc ggcgagcgcg agcgcaagga cgacgaacag
    29401 ccgacgcatc ggcggattgt cgcctctccg cagtgtgaag cgcgtgaggc ccgtcaaccc
    29461 ggcgcggcgg tcgcgcccgc cggagggacg ggcggaggcg gcgagggcgg ccgcgcgctc
    29521 gagtgcctgc cccatcggct tcgtgagcgg gaagaacgtg atcgtcgcgc ggcgcttcgg
    29581 cgtccgggcg tggaggacgt agccggtcag gcgccggacg aggaggggcg ccccgtcgga
    29641 ccaggagagg cgcccgacgc gcgtccggaa ccagccgcgg cacgcgaaga cggcgcctcc
    29701 gggaacgagg gcgacgtagc cgggaatcca ccgggggacg cccgggagcg aagcggcgta
    29761 ggccgggagg aggaggccgt cgcttcccga acggacgccc gcgtcggtga aggcgagggc
    29821 cacgtcctcg gggaggtgcg aggtgtcgag gccggggagc tcgcggtcga cgaccttgtg
    29881 ctggaagagg tagaggagcg ccgccacgcg ggagcggagg agcctccagc cgagcggggc
    29941 gacgagggcg aggagcgcga gcgcgacgac gacgaggacg agcgtgacga cggggtaggc
    30001 gagggcgagc ggcgcgatcg agaccgcggc gacgtcctcg aggaggctga ggccgacgcc
    30061 gacgccggtc gcgtgaccgc ccgtggccgt cgacgcgagg cgcgtccccg ccttgccggc
    30121 gtgggtcgag agggagacgc cgccggcgag gaggaggagc gggatctcga gcggcttcgg
    30181 gacgttcgcg ccggagacgg cggcccacga gatgaggacg gctccgagcg gccggacgaa
    30241 cgtgtggagg acgtcccaga cgttgtcgac cgcggggatc ttgtcggcca ggaactcgac
    30301 gacgtagagg agccccgcgg cgacgaggac ggccgggtgg gagaggacgg cgaggccttc
    30361 gaggccggcc gggagcgtca gccagtcgaa gcggaccgcg aggccggtga cgaggatcgt
    30421 ggcgtagagg ttgaggccgg cggtgaagct gaggccgagc gtcgtcccga ggagcgtcag
    30481 ggtctccatc gcgccccctt cccgggcctc tccctcgcgg gcggcccggg ggaatggtag
    30541 cgccgcgcgg gccgccatcc gagaccgccc ctagaatcgc gggatgtacg gagggaaccg
    30601 atgaagacgg cccggacccc gatcgctctc gctctcctga cgctcctcgc cggctcggcg
    30661 gcactcgcgg aagcccccgc ggcgatggcg ccgctcgggc tcgccgacct cttccgcgtc
    30721 cgcgtcgcgc gcgacgcgcg gatctccgcc gacgggaagg cggtcgcctt cctcgtccag
    30781 gtcccgagaa cgccggggaa gggcgaggac gggccggcgt ggacggagct tcacgtcgcc
    30841 gagcggggga aggccccgcg accgtacgtc accggggagg tcaacgtctc gagggtctcc
    30901 ttctcgcgcg acggctccga gctcttcttc gtcacgaagc ggaaggggga ctcgaccgca
    30961 gcgctctggt cgatccggag ggacggcggc gaggcgcggc gtcgggtggc gctcccggcg
    31021 gacgtggagg ggtacgccct ctcgccgaag gaggacggcg tcgtcgccct cctcgcgaag
    31081 gagaagaagg cggaggcggt cgaggcgcgc gagaagaagg gcttcgaccg ggaagtcttc
    31141 gaggaggaat ggcgcccggc gaaggtctgg gtcgcccagc tcgacggaga cgcgccgccg
    31201 cgcgctctcg acctgccggg ctcggcgtcc gacctccgct tcgcgcccac cggcgaccgc
    31261 ctcgcggtgg cgctcgcccc gacgccgtcg gtggacgacg gctacatgaa ccggaaggtc
    31321 cacgtcgtca cgctcgacgg gaaggtcgtc gggcgcgtgg agaacgacgg caagctcgga
    31381 agcgtcgcct ggagtcccga cgggacgcgc ctcgcgatga tctgcgcggc cgaccggaac
    31441 gacccgtcga cggccgcgct cggcgtcgtc ccggcgacgg gcggccggcc gaaggacctc
    31501 ctcgccggct tcgacggcca cccggccgcg ttcgcctggg cggggaacga ccggctcgtc
    31561 gtcgtcgcgg cgcgtggcgt cgcgaccgag ctgctcgagc tcccggccgc cggcggggcg
    31621 ccgaagaggc tcccgctcgc ggaggggctc gtcgtcgatg ccctcgatcg ggccccggac
    31681 ggaacgctcg cgttcgtggc cgagcggccg gaccggccgg ccgagccgtt cctgctggcg
    31741 gccggctcgg cgaagcccga gccgctcgcc gacctgaacc cgtggctcgc ccagcggcgg
    31801 ctcgggaagc agagcgtcgt ccgctggaag gcgcgcgacg gagtcgagct ggaagggatc
    31861 ctcgtcgagc ccgtcgagcg ggcggccggg gcgcgcgtgc cgctcgtcct cctcgtccac
    31921 ggcggcccgg aggcgcacgt ctcgaacggc tggctgacgc gctacggcga gccgggccag
    31981 ctcctcgcgg cgaaggggat cgccgccttc tggccgaact accgcggctc cacggggcgc
    32041 ggcctcgcgt tctcgaagac gagccagggc gacccggcgg ggaaggagtt cgacgacctc
    32101 gtcgacgcgg tcgaccacct cgtcgcctca gggctcgccg acccgaagaa ggtcgggatc
    32161 gccggcggct cctacggcgg ctacgccgcg ggctggggcg cgaccttcta ctccgagcgc
    32221 ttcgcggcgg ccgtgtcgtt cgtcgggatc tccgacctga cgtcgaaggc ggggacgacg
    32281 gacatcccgg tcgaggatgt cgacgtccac atgctctcga tgccctggac ccgcttcgag
    32341 cagagccggg agcggagccc cctctcgtac gtcgcgaagg cccggacgcc gctcctcctc
    32401 ctccacggga cggccgatcc gcgcgtccac ccgacgcagt cgcttctcct ctaccgctac
    32461 ctgaagctcg cgggccatcc ggcggtccgc ctcgtccgct acccgggcga ggggcacggc
    32521 aaccggcggg ccgcctcgca gctcgacgcg gccgttcgga tcctggggtg gttcgagcac
    32581 tatctcctcg gcgcgggagg tgctcctccg gcgcccgagg ccgcggacgg aaccgtcctc
    32641 gaggtcgtcg aggcggcgaa ggagtagagg atcgccggcc gtgccccgcg acgagggccg
    32701 tcgcggggca cggccggttt ctcgcacggc gccacggccc ggcgctaagc tacccgatgc
    32761 ggcacgcgga aggaggggga cgatggaaga gaccctcgcg ttcgtcatgg cgggcgggca
    32821 gggggagcgg ctccggccgc tcacggacgt ccgggcgaag ccggccgtcc ccttcggcgg
    32881 gatcttccgg atcatcgact tcacgctctc gaactgcgtg aactcgggcc tccgcaggat
    32941 ctacgtcctg acgcagtaca agtcgtactc cctctcgagc cacctgcgga ccggctggaa
    33001 catgctgagc ccgcgcctga accagttcat cgacgaggtg ccggcccagc agcagctcgg
    33061 aagcagctgg taccagggga ccgccgacgc gatccggcag aacttctcgt tcgtcgagag
    33121 caagcgcccg cggctcgtcc tcgtcctctc gggcgaccac gtctacaaga tggactaccg
    33181 gctcctgcgc gccttccacg acgagaaggg ggccgagctg acggtggcct gcgtgaggct
    33241 ccccgccgag gaggcgcgcg ggacgttcgg cgtcatgcag gtcgacgagg aggggcggat
    33301 cgtcgggttc gaggagaagc cggcggagcc gaagacgatc cccgggacga cggactgcct
    33361 cgcctcgatg gggatctacc tcttcgaggc cgggtgcctc cgccgcggcc tcgacaacga
    33421 cctcctcgac ttcgggaagg acgtcatccc gctcctcctc cgggacgggg tcccggtcta
    33481 cgcctacgac ttcacgacga agaacgcgat cccggagtgg gagtacacga agcgggacgg
    33541 ggtccggcgc aaggagctcg tcccgcgcgc gagcgacgcc gactactggc gggacgtcgg
    33601 gacgctcgag cagtactggc tcgcgaacct cgacctcgtc cagccgaagc cgcgcttcaa
    33661 cgtctacggc gagcgcttcc cgctctactc ggccccgggc cacttcccgc cggcgaagtt
    33721 cgtccacgag atcccgggac ggaccggcgt cgccgtgaac tcgatggtcg ccgacggcgt
    33781 catcgtgagc ggggggacgg tccgggagtc ggtcatcggg agcggcgtct acgtccacag
    33841 ctgggcgatg atcgagaaat cggtcctcct cggcggcgcg ctccggggag gagtccacac
    33901 cgagacgagc atcggccgcc gctgccgcgt gaagaacgcg atcatcgaca agaacgtccg
    33961 cctctcggag aacatctcga tcggctacga ccgcgacgac gacgagagga gggggctccg
    34021 caccgtcccg atcaccggca gctccgagca catcgtcgtc gtcccgaagg acttttcgct
    34081 ttagcggatc ggttcctctc tcggcgaacg ggcctctccg cgctacgatg gtcggctgga
    34141 ggtcggcgcg gatgggctgg ggccggaccc ggaagtcgag cggaggggag agcgcgccgc
    34201 ggccggcgga gaggatggtc cgcgtcgggt gccccgcgca caactgcggc ggccgctgcg
    34261 tcctcctcgc gaccgtccga gacgggcgga tcgtccgtct cgaggccgac gaccgccccg
    34321 acctcctcga agcgccgcag ctgcgcgcct gcgctcgcgg gcgctcgtac ctccgccgcc
    34381 agtaccaccc cgaccggctc ctccacccgc tgaagcgggt cggaaagcgc ggcgagggga
    34441 agttcgagag agtctcctgg gacgaggcgc tcgacctcgt cgcctcggag atgcgccgcg
    34501 tgcgggagac gtacggcccc ggcgccctct tcgtcccgta cgggaccggc tcgtacaacc
    34561 agacgaacgg ctcgcacgtc gcccggcggc tgatgaacgt cgcggggggc tgcctcggga
    34621 tctggaacag ctactcctgg gcggcgatca acgtcgcgac gccgacggtc tacgggacgc
    34681 tgacgaccgg gaaccagcgc caggactggc tcaacgcgaa gctgatcctg atgtggggct
    34741 ggaacccggc cgagatgcgc gacgggacga acagcgactt cttcgtgaag ctcgcgcgcg
    34801 agcggggcgc gcgcgccgtc tgcatcgacc cgcggcacag cctctcggcc gcggccctcg
    34861 ccgacgagtg ggtcccgatc cgccccggca ccgacgcggc gctgatgacc gcgatggcgt
    34921 acgtcatggt gaccgagggg ctccacgacg aggagttcgt gaggacgcac tgcgtcggct
    34981 tcgacgcatc gcagatgccg gcgggctgcg agggggagga gagcttcctc gcctaccttc
    35041 tcggcacgaa ggacggcgtc ccgaagacgc cggagtgggc ggaggcgatc acgacggtgc
    35101 cgcgggagac gacggcacgc ctcgcgcggg agtacgcgac gctccgcccc ggagtcctct
    35161 accagggcta cgggatgcag cggcgggcct acggcgagca ggtcgtccgg gccgggtgtg
    35221 tcctcgcggc gctcgcgggg aacgtcggcg tgagcggcgg ctgggcctcg ggcctcggga
    35281 accaggcgcc cgacggcggc ccctcctgga ccgtcttccc gacaggcgag aacccgttcg
    35341 gagcgtcgat cccctgcttc ctctggacgg aggcggtcct ccgcgggagc gagatggggg
    35401 aggcggacgg cgtcgtcttc tcggggagcg tcgcccgggg gcgcgacgcg gcggcggtgc
    35461 tcgccggggg gagagtttcg ccaccccccg gagtcgagga ggaatcgccg gctccgcccc
    35521 gccgcctcgg gaccgacgtg aagctgatct acgccgtcgc gacgaacgcg ctcgtcaacc
    35581 agcacgcgaa cgtgaaccgg acggcgcgga tcctggcgga cgaaacgaag gtggagtgta
    35641 tcgtcgtcca ggacaacttc ctgacgccga cggcacggtt cgccgacgtc gtcctccccg
    35701 cctgcacgca gttcgagacg tggggcgtcg aggacggctg gaagtacggc gacgaggtca
    35761 tcctgatgcc gaaggtcgtc gagccccccg gcgagtgccg gagcgactac tcgatctgcg
    35821 ccgacctcgc gcggcggctc ggtgtggagg aggcgttcac ggaagggcgc gacgagcggg
    35881 gttgggtgga gtgggtcctc gaccgctacc gcgagggccg cttccccgac ctgccgccgc
    35941 tcggggagct cctcgagaag aacgtcggcg tctgggcgaa gcccgtgacg cgccccgccg
    36001 tggcgttcgc ggagttccgg agggacccgg aggcgcatcc gctcgacacc ccgtcgggga
    36061 agatcgagat cttctccaag gcgctccacg agaaggggcg gcccgacgag atcccggccg
    36121 tgccgaagta cgtcgaggag cgggagagcc cgttcggccc ggaggcggag gcgtacccgc
    36181 tccaggtgat cggccaccac acgctccacc gggtccactc gacgcacgac aacgtcgact
    36241 ggctggagga ggcgttcccg cagcgcgtct tcctgaaccc gctcgacgcg aaggcgcgcg
    36301 ggatcgacga cggcgacgag gtccgcgtct tcaacgcgcg gggcgccgtc cgcctgcgat
    36361 gccgcgtcac gccgcggatc ctcccgggcg tgatcgacat cccgcagggg gcgtggtgga
    36421 cgcccgacga gcgaggcgag gaccgccgcg gcaacgtaaa cgtcctgacg agcgagcgct
    36481 ggacgccgct cgccttcggg acggcgcagc acacggcgat ggcgcaggtg gagcgcgtcg
    36541 cggggccgga gggggcgcgc ccccggcggc gccggccccg ccccgccgcc gcggccttcc
    36601 ggcgcctgga gcgaccgcgg tgaaggggcc gtacgccgtc cacttcgacg cctcggcctg
    36661 cagcggatgc aaagcctgcc aggcggcctg caaggaccac aacgaccttt cggaggggat
    36721 cctctggcgg cgcgtctacg aggtctcggg cggcggcttc cgcaaggacg gcgccgcgtg
    36781 gcgccaggac gtcttcgcct ggagcctctc cctctcctgc aaccactgcg agaagcctgt
    36841 ctgcgccgag gtttgcccga cgggggccgt gcgccgacgg gaggacggcg tcgtcctcct
    36901 cgactccgga aagtgcatgg gctgccgcta ctgcgagtgg gcctgcccct acggcgctcc
    36961 gcgcttcgac gaggcgcgcg gcgtgatgac gaagtgcacg ctctgcgccg accgcctcga
    37021 ggacggcctc gccccctcct gcgtcgccgc ctgcccgatg cgggcgctcg acttcggctc
    37081 gctcgaggag atgaaggagc gatacccggc gccgaccggc tttccgcttc ccgatcccgg
    37141 cctgacggag ccgtgcttcc ggatgacgcc tcatccggcc gcggcgcgct cggagtggga
    37201 aggggagatc gcgaacgtgg aggaggtgga gcggtgaggg aggcctcgct cgtcgccttc
    37261 acgctccttt cgcaggcggc cgtcgggatc tgcggggtcc tcctcgcgct cgcgctccgg
    37321 gccgagaggg ccggcacgtc cgcggccctc gaggccgtgg cgctcccgct cctcgccgcg
    37381 gcgctcgtcg ccgcggtact cggcctcgcg gcctcgttcc tccacctcgg cagcccgcgc
    37441 aacgcctggc gggcgctccg gaacgtccgg acctcgtcgc tgagccgcga ggtcttcctc
    37501 gcgtcgctct tcacggcggc gctcgccgct gcggtcctgg tccgcctgcg gacggatccg
    37561 ccgggtttcg ccgctggctt cgcggaaggc ttcgccagcg tcctcggggt cggtctcgtg
    37621 atgacgatgg ccggggccta ccggctccgg acggttccgg cctgggaccg ggtggcgacg
    37681 ccgctcgcct tcttcggctc ggcgctcgtc ctcggcaagc tctctgtcgc cgcggccctc
    37741 gcgctgcggg gtccggatgc ggcctcgggt gcgtgcaccg tccgcctcgt gatcggcggg
    37801 ctcgccgtcc tcctcgcggg gctcggtgtc acgctcttct ggctacggcg gatcggccgg
    37861 gaacgtggag cgtcacgcga ggcattcgac cggacggcgg gggcgagccg acggctcgtc
    37921 gcggcgagga tcgtcctgac ggccctcgcg gcgggcgccg gcctcggcgc gttcgcggga
    37981 gagctggcgg cgtggttcgt cgtcgcgacg ctcgtcctcg ccttcgcggc ggagaccgcc
    38041 gggcgcgtcc tcttctacga ggcacgggtc cggtccggtc tctgaggggc cgcggtggga
    38101 gcgaggaaga ggacgggcag gagaggcccc ggggtgtacg gttcgcgggt ttggagactc
    38161 atgcggggga ggcgtcgcgg cgcgtcgccg acgccccgag cggaagggag cctcgatgaa
    38221 gacggtcctc gccctcgtat tcgcccttct cccgttctcc tcggccttcg gccaggagcc
    38281 ggagccgctt cccgcggcgc gcaggagcgg cgggatgccc ctgatggagg cgcttgcgaa
    38341 acggtcgacc tctcgtgcgt tcgacgcgaa gccgctgacg acccagcaag tcgccgacgc
    38401 tctctgggcc gcgctcggcg tcaaccgttc cgatgggaag cgaacggccc cgacctcgcg
    38461 ggaccgccgc gagatggagg tctacgtcct gcggtcgagc ggcgtctcgc ggtatgacgc
    38521 gtcggcgcac cgcctcgtcc ccgtcgcgtc ccgtgacctc cggacggtcc agagcgccca
    38581 ggagttcgtc tacgacgccc ccgtcacgtt cgtctacgtc gccgacctct cgaagatgtc
    38641 cgacgggagc cgggcggcga aggaggcgac ggcctcgttc gacaccgggt tcgtctcgca
    38701 gaacgtctac ctctggtgcg tctccgaggg gcttgcgacg ggggtccgct cctgggtcga
    38761 ccgcgacgtc ctcgcgaagg ggatgaagct cggcccggac cagtggatcg tcgcggctca
    38821 atcgatcggc tggccgaagc gctgacccgg gccgtccgcc ccatcacggg gacgccgaag
    38881 gcgcgaaggg ggtggcgcga cgactcggag cgcgcgaggc cgcggaggcg gcgcaccggc
    38941 tcccgggccg cgcgtccggg aatgcgatga tgcccggcgt atcgatcgaa ccggacacga
    39001 acggaggact cgaatgctga atcgtcttct cgccccgcga acggaggccg cctacgccct
    39061 cctccgcgtc gtcgcgggcg tcctgttcgc cctccacggc gcgcagaagc tcttcggcgt
    39121 cctcggaggg accgtccagc cggccgggac gcagctctgg ctcggagcgg tcatcgagct
    39181 cgtcgcgggt ctcgcgatcg cggccggcgc cttcacgagc tgggccgcgt tcctcgcgag
    39241 cgggacgatg gcggtcgcct acgtgcagtt ccactggaag ctcgcgttcg acgccggctt
    39301 cttcccggtc gtgaacaagg gcgaaatggc gctcctctac gcgctcctct tcctgttcgt
    39361 cgcctgccgg ggcgccgggc cttgcggctg ggacggccgg atgggctcga aggagagctg
    39421 agcctcggca tccccggccg gcgcggcgcg ccgggagcgg ccggagcgcg ccgcgggaac
    39481 aggggcgggc gccgcgcgag tcgaggctcc ttccgcgcgc ggccacgcca cggagacatc
    39541 gaatcccgcc ggagcccggg cgcggagggc ttccggccgg aggagcggca gcgactccgc
    39601 gggcggctcg gcgaggagac ggagccggac gaggcttcgc ggctcgagcc gcgcgagcgc
    39661 cgcgtcgatc cgctcggaga gaccgggagg caccgggtcg atctcgacga cggccatcgg
    39721 ccggacccgg tgctcgcgga acgtccacgt cgcacggccg ccaggggagc cgtccgacga
    39781 gagctcgagg aggaagctcc ccttcggctc gaagcgctcg gcgaaggaga cgcggtcggt
    39841 cgagccggcg aagaggaccg gcgccgcaag ggggcgcccg gcgaggtcct tcgtgaggac
    39901 ctgcgcgcga tggatgtggc ccgacaggac tgcggcgaag gcgggcggga cgtcggcgcc
    39961 gcgaaggacg tcgactcccg agcgaaagac gtagtccgac gggccgacgg tcgccccctc
    40021 gatcgcctgg tgaaggcaga ggagccggac gtcggcccga accgaatcgg cgtccgtctc
    40081 ggcgagaagg gcgggggcga tgtcgcggac gcagggcgcg tacgggaagc cggagagggc
    40141 gagcgtcacc ccgtcgcgcc gaacgacgaa cgtgcgcggg cggtcgaaga ccttcacgcg
    40201 cgggtgcgtg aggaggagct cgcgcgggat ggcgccccgc tcgtggttcc cggggaccca
    40261 gaagaggtcg acgccggagt cggcgaggtc ggcgaggcgg gcgaagaccc gctccgcgag
    40321 ccaggccggg acccggctcc ggtagaagag gtcgccgccg tggatgacga cgcccgcctc
    40381 gccgcgtatg gccggctcga gcgccctctc gaagctcgcg aacaggtcgt cgccgcggcg
    40441 cggacggtcg acgcgcggcc gcccgggaag gtcgagcccg aggtgggtgt ccgacaggaa
    40501 gaggacccgg agcggcaccg gaggaggcta ctccgttccg gaaacggaga gcggggccgt
    40561 acggccccgc tcgtggagga cggaggcgga ggcggggccg gccggggccg gccccgcctc
    40621 cgcggaaggt caggagacgt gagcggcggc cggctcctcg cggggagcgg ccttcccctt
    40681 ctcctccttg aggtacttgt cgaggatccg gctcgcctcc gccggcgtca cggggccgta
    40741 gacctggtcc ccgaccatca tgacgggcgc caggccgcag gtgccgaggc accgggcgct
    40801 ctcgagcgag aacatctggt ccttcgtcgt ctcaccgagg tggacgccga ggtcgtcgga
    40861 gaggcgggca acgagcttgt cggccccctt cacgtagcag gccgtgccga ggcagacgtt
    40921 gatgacgtgc ttgccgcgcg gctggaggcg gaagtagtgg tagaaggtcg cgacgccggt
    40981 gaccgtggcg tacgggatct gcgcgagctg cgcgacggcg tgcatctgcg tctggccgag
    41041 gaacccgaag tgggcctgga ccatgtgcag cgtcgcgatg aggttgctct cggggtgctc
    41101 ggtcttcgcg ttcttctcga tgaactcgac gatgtgcgac ggcaggatgc gcaggctggt
    41161 cgccttcact tcctcccagt tgtcggtgca ggcggcgtgt tcgctcaccg gatccccctc
    41221 ggcatgcggg cctggtagtg cgtgtggagc agctcgtggg ctttcttccc gttcggctcg
    41281 ccgaggaact cctcgtagag gtgctctatg gcggggttct cgtgggagcg gcggagctgc
    41341 ttgtgagagt cgatgtcgta gagggcgcgg gctcggagct tggcgatctc cgggtccatc
    41401 gggtcctcgt cgagcggcgg gtagggctgg ccgccgccca tgatgcagcc gcccgggcag
    41461 gccatgatct cgatgaggtg gtacttcttc ccgccggcct tggcggcgtc gaggatcgcc
    41521 ttcgcgttcg tcaggccgtt cgagacggcg atgttgacgt cgaggccgtt gatgtggacc
    41581 gtggcctcct tcaggccctt gacgccccgg acttcctcga agacgagcgg gccgagctcc
    41641 ttgcccgtca gcttcacggc cgcggtgcgg agcgccgctt ccatgacgcc gcccgtggcg
    41701 ccgaagatgt cggccgcgcc ggagctgatc ccgagcgggc ggtcgaagtc gccgtccggc
    41761 aggctctcga agtcgatgcc gtacgacttg atcatccagg ccagctcgcg cgtcgtcagg
    41821 acggcgtcgg tgtgcggcgt gccgtcgggc atccggtgct cgggacgcgc cgcctcgaac
    41881 ttcttggcga cgcacggcat gatcgagacg acgtagacgt ccttcgggtc gagccccttc
    41941 ttctcggcgt agtacgtctt caggagcgtg ctcgtcatct gcatcggcga cttgcacgtc
    42001 gaggtgtggg ggatcatctc ggggtagaac ttctcgagga agttgaccca gccggggctg
    42061 cagctcgtga tgagcgggag gttctcgtcg cgctggaagc ggccgaggaa ctcgttggcc
    42121 tcctcgatga tcgtcaggtc ggcgccgaag ttcgtgtcga agacgccgtc gaagcccatc
    42181 cggcggagcg cggtgacgag cttcccggtc acgggcgtcc cgaccggcat gccgaagcac
    42241 tcgccgatcg tggcgcggat ggcgggggcg gtgtggacga cgacgtgctt gacgtcgcga
    42301 gggagggcga gcgcctccca cacgcgctcg gtgtggtcct tctcgaggaa ggcggccgtc
    42361 gggcagacgg cgacgcactg gccgcattgg atgcagaccg actcgtccat gttcgcgagg
    42421 ttcgccgggc cgacgacggt gtggaagccg cgcccgtgct gggagaggtt gctgactccc
    42481 tggacctcgg agcagacgcg gacgcagcgg ccgcagagga tgcacttctc gggcgtcctc
    42541 acgacgctct ggccggagtc ctcgacgggg agctgcttgc gcttgccggc gaagagccgc
    42601 tcgcggatgc cgagccggta agcctgcttc tggagctcgc agttgccgtc ccggtcgcac
    42661 gtctggcagt ccatcgggtg gttgtcgagg aggagctcga cgatgtcgcg ccgggcctgg
    42721 cggatctccg ggctgttcgt ctggacctcc atcccctccc agacctcctg cgagcaggcg
    42781 gtcaggaagt gattgaagcc cttcacctcg acgacgcaga cgcggcaggc gccctcgatc
    42841 gaaagcgcgg ggtggtagca gaggcggggg atgtggatgc cgatcttctc ggcggcgttg
    42901 atgaccttgg tccctgccgg gacctggatg aactcgtcgt cgatcttgag gttgatcatc
    42961 ttctggctcg tgggcatgtc ggccgtcctt cgctcagacc cgctcgaccg cgtcgaaccg
    43021 gcagatctcg tagcactggc cgcacttgat gcagcgggcc tggtcgatca cgtgggcctt
    43081 cttcctctcg cccgagatgc actgcgtcgg gcagttgcgg gcgcaggcgg tgcagccgat
    43141 gcacttctcg tcgtcgatct ggtactggat gagcgcgacg cacttcttcg cgcggcactt
    43201 cttgtcgacg atgtgctcgc ggtactcgtc gcggaagtgc ttcagcgagg agaggaccgg
    43261 gttcggcgcg gcgcggccga ggccgcagag gctcgtccgc tggcagagct tgccgagccg
    43321 ctcgagccgg tcgaggtcgg agagctcgcc gttgccggcg gtgatcttct cgaggatctc
    43381 gagcatccgg gtcgtcccct cgcggcacgg ggtgcacttc ccgcagctct cctcctgggt
    43441 gaacgtcagg tagaacttcg cgatgtcgac catgcagtcc tcgtcggaca gcacgatcat
    43501 tccgccggag cccatgatgg agccggcctt cgcgagcggg ccgaagtcga cgggcatgtc
    43561 ggcgcggttc gcggggatga agcctcccgc ggggccgccg gtctggatcg ccttcagctc
    43621 gcggtcgtcg aggacgccgc cgccgatgtc gaagacgacg tccttcagcg gcgtgccgag
    43681 cgggacctcg acgaggcccg tgtgcttgac cttgccggcg agggcgaaga ccttcgtccc
    43741 gccggaatcg tcggtcccga cctgcgagaa ccagtcgccg ccgaagttga tgatggcggt
    43801 gacgttcgcg aacgtctcga cgttgttgat gcaggtcggc ttgccccaga ggccggccgt
    43861 cgtcgggaag gggggcctca cgcgcggctg gccgcgctct ccctcgatcg agcggatgag
    43921 ggccgtctcc tcgccgcaga cgaacgcgcc cgcgccgagc cggatctcca tgtcgaagct
    43981 ccagccgctc cccatgacgt tctcgccgag gatcccggcc tcgcggcact tctgaagggc
    44041 gttctcgatc cgcttgatgg cgagcgggta ctcggcccgg atgtagtaga agcctttgca
    44101 ggcgccgatg gcgtaggcgc cgatgatcat cccttcgacg acgttgaacg ggtcgctctc
    44161 gagcatcccg cggtccatga aggccccggg gtcgccctcg tcgccgttgc agatgatgta
    44221 gcgggtcttc tcctcgttcg cgcgggcgaa cttccacttc tggccggtga ggaagcctcc
    44281 cccccctcgg ccacgcagct tcgccttcag gacctcgtcg acgatccagg tcgggtcgcc
    44341 cttctcgagg cacttcgcga gcgccttgaa gccgccgaag cggacgtact cctcgaggct
    44401 ctcggggtcg aggatgccgg cgttgcgcat cgcgatccgg ctctgcgcgt tgaagaacct
    44461 gacgtcggcg tagaggtcga agaagtcctg cgcgatcggc ttgaagggga ccttcatcct
    44521 ctcgtcgatc gtccccttgc ggacgggtcc gtcgaggagg gcgtcgagat ccttctcgtt
    44581 ctcgagcctg tagatcgtct tcgagggacg gacgaggacg tgcgtcgact tcccttcggt
    44641 ctcctcggag cagacgccga ggcaggcgtg ggagttgacc ttcacgcggt ccgggccgat
    44701 cccggccgcg gcgagcttcg cctcgaagga ggccttcagc tccgtccgct gcttgcagcg
    44761 gcgcccctcg cagaagacga gctcgtactc gtacggcgtc tcgagcgagc cgtcgagcgc
    44821 gtgctcggga agggcggcgc ccttctggat gtggctcgtg aagatcttgt ggacgagctc
    44881 gcggtcgacg cgctcgtact tgacgggggg cttcccgggg accgagacgc cgacgatcgg
    44941 ctccttgctg cagcggccgg tacagccggt gcggtggagg acgacgtcct cgcgccccga
    45001 ggaggcgagg tgcttgacga actcgtccgc cacctcgcgc gagccggcgg cgtgctcgca
    45061 ggtggcggaa ccgatctgga cgacgacctt gccggcgtct ccccgcaggg cgagcgcctc
    45121 ctcggaccgc gccgccagtg attgaaaggc ttgggtcacg gcgtttggca tttgggctcc
    45181 atcgatggct cggggaggct ccgggcctcc cgcgagcgga aacgggacgt ttcgacaggt
    45241 cgcagcgcga ccgttcggcc cgcgctctgc gggacggccg tcggctgcgg gaatcgggac
    45301 cgatttgggc tggattgtcc ggaacgggag agacgggcac ggcgcgggag acgccgtgcg
    45361 cggtccatca ggcgccgcct gctccggttc gcacgggttg cccgtgggcg ggagcggccc
    45421 ggcgagagga gcaccgggcc gggtgaacgc cagcgtcgac gcgtcgggcg cggtcgatcc
    45481 ggaatccggt cctggggcgg tccttcggcg tgctcatgat cctgggcgcg cccgatgcct
    45541 tgccgggcgc gagggggctc cgtttccggt gcctgccccc gtttcgggcc gccctgatct
    45601 tactccggtt cgaccgaacg gcaggtggga ggccaaccat ccgagacggg cctgccgcca
    45661 gcggaacgtt gagacgaagg cgcgaaaggg cctttctcga cggccgctgc tcagccgccg
    45721 tgcggcctcg acgccgcttc gagccgatcg ctctcttcgg cggtcaggga gagcgtggcc
    45781 gccggcagga tctcggcgag ctgctccgga gtccgggcgc tcgcgatcgg cgcgacgacg
    45841 cctggccgcc cgaggagcca ggcgagggcg atggacgcca cggtcgtccc gcgctccgcg
    45901 gcgaccgcgt cgagcgccgc gaggacgcga agacccggtt cgtcgaggtg cttcgcggcg
    45961 gcgccggagc gcacgctggc gatggtgcgt ccgggcctgt acttgccggc gaggaagccg
    46021 ctcgcgagcg cgtaataagg gaagacggcg aggccttcgc ggacgcagag cgcctggagc
    46081 tctccctcgt actcggcgcg gtggacgagg ttgtagtgcg gctggagcgc gacgtagcgg
    46141 gcgagcccga gccgccgcga gacggcgagc gcctcggcga ggcggggtgc cgcgtagttc
    46201 gaagcggcga tctcccggac ctttccctcg cggacgaggg cgtcgaaggc tccgagcgtc
    46261 tcctcgagcg gcgtggccgg gtcgtcggcg tgggcgtagt agaggtcgat ccggtcgatc
    46321 cgaagcctcc ggagcgagtc ctcggcggcc ttccgaatcg tcgcggccga gagtcccgag
    46381 aggccggcca tcttcccgac cttcgtcgcg acgacgaccc ggtcccggtt cccgcgcgac
    46441 gccatgaagc gcccgaggat ctcctccgac tcgccgccgg agttgcccgg cttccaggcc
    46501 gagtagacgt cggccgtgtc gacgaagttg ccgccggcgg cgaagtaggc gtcgaggacg
    46561 gcgaacgact cggcttccgt cgccgtccat ccgaagacgt ttcccccgag gcagacgggg
    46621 aagacctgaa ggctcgtggt gccgatccgc tgcatgggac ctccttcgcg ggacctcttc
    46681 cgggaggatc ccacagcagc gggctccggc gaggcgcccc cgcaaccgcg gcgaccggtt
    46741 actctcgcga acggcggcct acggccgccc ggagacccga tgctcaggat ccttcgttac
    46801 gcacccgtca tgatgttcct cgcggggacg gccctcgggg cggccccctc atcgcaggca
    46861 cccgcggaga aggacgtcgc ctcgtcgggc ttcgccggca cctgggcgct cgcggcgacg
    46921 gcggacgacg tcgcgcgggt cgcgccagcg aacgcctggg gagggctcgg cgggacgacc
    46981 ggcgcgacgg ctcggccggg cgggggcgga ttcgacctgc cgatcgaggt gatgacggac
    47041 gcgcgtctcc tcgtggtcga ggacgacggc ctccggctcc gggtcaccta cccgtcgggg
    47101 cgcgtccgga ctttcgtgac cgacggaagc aaacgctacg tcgacgacgg cgacggcccg
    47161 gccgacgtcg tcgcgcgccg gcgggaccgg acggtgacgg tctcgtccga atgggtgcgc
    47221 ggttaccggc tgcgcgagac gtgggagctg cggactgacc cgcaccggct cgtcgtgacg
    47281 ggacggctga aggggaggga gtcgcacgag tacgtgagga actacgagcc ggcccccgaa
    47341 ggagcggtcg ccgcgagcgc gaccccgcct ccgccggccc cgacacccgc cgtccggctc
    47401 ccctgggcgc cgtccgttcc ggcatcgacg cccgcgccgg cgccggagga gccgggcggc
    47461 tcgcccgcga aggagccttc gtccgtcgac cgcctctcgg agtgcacgct ccgcccgccc
    47521 gcgaggactc ctcccggggc gctgaacggc ctcgcgaaga caccgcagga gaccgccgcg
    47581 aaggcggccg ccgcgtccgt cgcgccgctt cgcgcggacg gcgtcctctc ctccgacgcg
    47641 gaagtcttcg acggctgcct ggtctggccg ttcaccctcc gcctgccggc gctcggcggc
    47701 gtccaggagg tcttcgtcga cgcgggcgac gggcgcgtcg tgaagtcgga cttcatccgc
    47761 ctcggcccga ggccggccgg agctccttga cggaagccgc cccgaccgcg cgcccggggc
    47821 ggcgcgccga tcagtgtccg gccggcacct tctcgcccgc ctcgacgcgc tccgggagga
    47881 cgttcctcgg catcacgagc gggcaggtcg cgtagggcgt gaaggcgcag ggcgggttct
    47941 gggcgaggtt gaagtcgagg acgaccttcg aagggccggc cttcggcgcg gtcgcgtaga
    48001 ggaagcgtcc ggcgccgtag gtttccgttc cgttcgtcgc gtcgccgaag acgaagaaga
    48061 gggtgtcgtc gtccggcgac tcgacggtcg gctcgagcgt ccgctccttc cccccgatcg
    48121 tgaaacggac gagacccggc gcgagagact tctgggccgg accctgggcg gaagggacct
    48181 cgatctcgcg gggcgcgtcg tacggctcga acgtcccctc gacccggagc gacgggtcga
    48241 cggggaagcg ctcgattccc cggaactccc tccgccgcgg gctctcgggg tcccgggccc
    48301 ggacggcgag cttgccgcct cgatcgagga ccgtcaggcg gacgcgtccc acctcgacga
    48361 cggagggttt cccgtcccgg tcggtggcga gagttgcgcc ggtcggcggg gcgccgtcga
    48421 cggtgacggg ggcgccggca tccacggcga gccggacggc gccgtcggga ccgagaacga
    48481 acgccccggc cctcgccggg atgccgggcg cttccagcga gagcgggagg gcggggtccg
    48541 agccgaacgg attttcgccc ggagtcagcc agtggagggc gacgagcgtg agccagccgc
    48601 tctcggaagc gagcctcgtc tcgcgcagcg cccaggccgt gccgatctcg gccgcgtagg
    48661 ccgggtccgc ggccggaggg cggcggcagg cgacgagggc gacgacggcg acggcggcga
    48721 ggaggaggac gggacgggcg cggcgcatgg ccccttctag cacggcgacc gggagagcgt
    48781 ccgttgagcc gaagcgctgc gagccggcga cgacgatcag gggtgcgaag gagccttctc
    48841 gccggcctcc acgcggatcg gaagcgtgtt cctcgggagg gccatcgggc agctcgcgaa
    48901 gggcgtcagc gagcacggcg gcgtctcggc caggttgaag tcgaggacga cgcgagccgt
    48961 cccggccgcc ggtcgcggga cggagaggaa gcgcccgccg ccgtagctct cggagccgga
    49021 ggtcgcgtcg gcgaagacga agaagagcgt cgcgtcgtcg ggcgactcga cggtcggctc
    49081 gagcgagagc tccctcccgc cgagggtgaa ccggacgagc ccgggcgcga gggccgttcg
    49141 ggccggccct cgtgaggagg gcacctcgac gtcgcggggg gcgtcgtacg gctcccagcg
    49201 cccttcgaca cggagctcgg ggtcgatcgg gaaatgcgag aggccgggga agccgcggag
    49261 ccgcgcgctc tcggggtcgc gggcgcgcac gtacggcgcg ccgtccttca cgaagtacgc
    49321 gaggcggaca cgccccacgg tgacgacgtc ggcgcgctcc tccccctcgg ggacgagcgg
    49381 cgcatcggtc ggcgcgcctc cgttcaccgt gaccggcgcg cccggctcgg cccgaaggac
    49441 gacggcccca tcctccctca cgacgaacgc ccccgcccgc ggagggattc cgtccgcctc
    49501 gagcgcgatc ctcgacgacg ggtcggagcc gaaggtgttc tctcctggcg cgagcggttc
    49561 ccgcgccacg atcgtcagcc agccgtcctc cgtcgtgagc tcggcgtgcc gggcggcgcg
    49621 cgccgcctcg atctcccgca cgtagtccgg gtcggcggca ggagggcgat cgcaggcgac
    49681 gaggagagcg aggagggaga gcagagcggg cgcgaggcgc atgcccgttt ctagcacggc
    49741 cccgggagcc gtcacggcgg gggagggctc gcgagcggca ggctgaagga gaagacggcc
    49801 tccgggccgg ggtgcttcag ccacatccgg ccgccctgcg cctcgatcgc gaggcgacag
    49861 aacggaagcc cgagcccgga gccgcggccc ggaaggcttc cgacggcgaa cttctcgaag
    49921 aggcgcgggc gcagctcttc gcagatgccc ggtcccgagt ccttcacgtc gaggacgacg
    49981 ttcgccccgt cgacgcgcgc cgccagtcgc acggagccgg agccgggcgc gctgaacttg
    50041 accgcgttcg tcaggaggtt ctcgacgacc cgtgcgacga ggccggggtc ggcgtgcacc
    50101 ggcggcaggt cgggcgggag ggcggagacg agcgagaggc cgcgggcctg cgcggcgggc
    50161 agcgccctcc cgatcacctc gtcgacgacc gtcgggaggt gaacgtcggt ggggtgaacg
    50221 ggcagcgacc cctcctcgag ccgggagagc tccagtatcc cgtcgacgag gctgagctgg
    50281 cgccgtgtgt tccgctcggc gaggtcgagg agcttccttc cgtcctcgtc gagggaccgg
    50341 ttctcgcgga ggagctcgag agcccccatg acgcccgata tcgggccgcg aaggtcgtgg
    50401 acgagcatcc gcgaaagctc ctcccgggcg cgcgcgagga ccagcttccc gaggccgagg
    50461 ctgatcgcct cggccagcgc ttcggcgaac gagcaggcgt cgtcgtcgag gccgtcgccg
    50521 gcggcggcct cgaagctcgc gactccgagc cggttgccga ggtgccgcag cgggacgacg
    50581 aggccgcgac aggtctgtcc cgcgccgtcg ccgctgatcc gcgtgacgag gccggtctcc
    50641 caggcgagcc cgagctcgcc cgcgagcgtg gagggccgca ggtcgccgag gccctcagtc
    50701 cggccgagaa ggagaaagcc gtcggcggcc ggaagggcga cgaagacccg ggagaacgac
    50761 gacagccggc agatggccgc ggccgcctcc gcggcgatct cctccgaagc gtgccggcgg
    50821 gccgagacgt cgaggacggc gttcaggacc tccagcccgc gcgtggcgcg tctccgcgcg
    50881 tcggcgctct ctgccgtgcg gagcgcgagg gcgatctgag ccgcggtcga ctcgatgagg
    50941 cttcgctgct cctctccgaa gtgtccggcg tcgggatgga cgagggtgag gactccggcg
    51001 agggaggttc ccgtcgcgat cggcgccgag agcgcggacc ggatcgtccc ctcctgcgac
    51061 gccagatgcc gccaacggtc gtcctccagg acgtcctcga cgacggcgat cgtgccgctc
    51121 ctcacgaccc agccggccag gccggtctcc agcatctcgg ccgcctcggc cggcccgacg
    51181 acgagagtgg agtcgccttc ggtgaagagc ccgcgcgtga agcggccgtt cccgtcgagc
    51241 agcagcgcgc tggcgccgtg cgccccggtc gcgactttcg cggcgtcgag gactttcgcg
    51301 agcgtcccgt ccaggccctc ttcggccccc gcggcccgcg cgacggagag gaggctcgcg
    51361 aggaggtcgt tctgggtccg tacgaccctc gtcagccgct ggctcgtggt ggcgaggagc
    51421 gtcgtcgtga agaccacggc gaagatcgtg cagaggagcg gtgggagtgc gaggagcgga
    51481 cctccggcga ggatgtcccc ctcgacgaag ccggcgccca cggcgacgcc gcgggcccag
    51541 aagagggcag cgcaggccca gagcgcgacg gccgtgacct tgtcgagcgc ggcggcgccc
    51601 tgcggggcgc ggcgtcgcga ggcggtccac gccgcggcgc cgaggagcgc ggcgaggaac
    51661 gcgctgctca ggatcgcccg ggcgttgccc gcgtcgcgga ggaacgtcgc gccggcgaaa
    51721 ccggcggttg cgaggaggcc cgtcccgacg gtcaggcgcc ggctggggcg gaggccgtag
    51781 tagagcttcg tcccctcgag gatgaaggcg gcggcgaccg ccacgaggac gttcgcgagg
    51841 acgtcgggga ggaggtccgg aatcctcccc cggaacgcga ggaggacgag gccgaggaag
    51901 aagaggccga agccgagcgc gagacggtcc ggtccgcgga tcgcgcggag ctcccgccag
    51961 gcgagcgcga ccgcgagcac cagtcccgcg aagatgacgg cgctcgccgc gaggagcgtc
    52021 cgcgcgtcga agagaggttc catcgccgcc acacatcata cgagccggac ggggcatcgt
    52081 cctcgctccg gacggcgtcg gggcgtgccg actgggcgac gggtggacgg atccgccgag
    52141 gtgtcgccgc gaagaccggc tcgggaccgg agctccctgg gaagggcagt ccgggtcggg
    52201 cggggggggc gttttcgacg gcgccggggt gtcatagctc cttagaaatg tcaccccctg
    52261 gaactcgata gaaatgtccg ttt
//
LOCUS       DAOONL010000017        51009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_469_length_51009_cov_17.196668, whole genome shotgun sequence.
ACCESSION   DAOONL010000017 DAOONL010000000
VERSION     DAOONL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 51009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51009
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_51009_cov_17.196668"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLP50_04545"
     CDS             <1..575
                     /locus_tag="PLP50_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPA50852.1"
                     /translation="LFLFYGDLPLKGDAVFPKFIVEQVPSGLSGLIIAGLLAAAMSTV
                     SGSISSISSSTVFDLWASTKRGRLASEEEKLRLSRRVSFGWAILLTGAALVFIGTGQA
                     VVELALSIASFTYGSFLGLFLLAVRGRTSRRAAAAGFLAGVFVMVFVVFGTPLAWPLY
                     TLVGSLATLLVALAVDRLRPERPAVGEAGA"
     gene            572..1243
                     /locus_tag="PLP50_04550"
     CDS             572..1243
                     /locus_tag="PLP50_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HPA50853.1"
                     /translation="MSRGSLAFCGALAAVALLLAVPSSQAWVVETTRAHPYAMGFLKF
                     AILASLGELLAIRVVSGGWKLPAGPAWRAVVWGLLGAALALLFPVYAAGVPAAIGAGL
                     LPVLPGALGAKVATACWVSAVMNLSWGVALMLLHRLTDTWLDLADGALARVPSVGVGA
                     VAAAIDWKGFLGFVLAKTIPLFWIPAHTVTFLLPPELRVLFAAFLSLALGAILAFAKR
                     RAAAG"
     gene            complement(1430..>2501)
                     /locus_tag="PLP50_04555"
     CDS             complement(1430..>2501)
                     /locus_tag="PLP50_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50854.1"
                     /translation="ALSGAFTITAGPASQLVYIQPPTPAAPGATITPAVTVQLRDAAG
                     NPVAVAGTTITISLTNGTGILSGTASQLTDAAGLATFADLSIDTSGPKQLTAASAGLT
                     SAVSSTFSITAAAATQVAFVQQPTDAASGATITPAVTVQLLDAGANPVAIAGTTVTLS
                     LTSGTGTLAGTASQVTNASGLATFADLSIDLAGAKQLTAASAGLTSALSGAFTITAGP
                     AAAIAVTAGSLQSAPADGAFLAPLLVTVTDAGGNPVSGVTVTFLAPISGPSALLSNGG
                     IATTNAAGQAWVTATANGIPGGPYIVTASIGALPAAAFALTNLSADAIANVPVLGGFG
                     IGVFALLLAAVGALASRGVFKV"
     assembly_gap    2502..2601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2602..5398)
                     /locus_tag="PLP50_04560"
     CDS             complement(<2602..5398)
                     /locus_tag="PLP50_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50855.1"
                     /translation="MTHVSRSTVAPGFCTTMGPRLRRRLAVALSVVGAAIAVATPVEA
                     GDSTITAPRIVYTGVGQTIEFTGTDSISNESRVLTMDASISGSCNPTAGNGWSISLCG
                     RVQISLTDGTGGTLFMAGTTQVQSSPVVYRTASGAIVENPTDPAIWGGPVLTVYMNGT
                     LDQLNGALADLEYIPAADYEDGTYDEVTPPGAGTPNIDILVNDGSVSSTNATAEIILR
                     VEGPNGGPTLAGPASALTAAAGVENAYPPSTSDPALFSVVDPELCLRSPNNRCDGAYD
                     AAPAPLIPEPHDAMLLVAWLPQAGCGQFALRSVSGFANLGGAAFPSVNAILTSPTGLD
                     LEQPAADAILATLGLAGAVNLSGQPSGNNLTTVFAGTAGSIADVRYALSQLTYAAPAA
                     ETSCTLNFAFADLGNNGMPQEWVPPDPPVGEYEIPNALASSISVTFNVVDTHPDVTID
                     QIIPSLAGDPAGPNKPSGFRITFGEPIDPASFDTSDLSLATSSASGAAFGALVPVTPG
                     LVYTVPVTATGDGTITLTMAAGGACAAGHFSGSCDAGYDSEAPVYSDNEIEWDQTAPT
                     ATMVAEAGQPDPTSAATVYFTLDVSDTVSTAPLDIADSEIGLSGTANPTTAAVSWPDI
                     TKPKQFRVAVSGMNGTGTVAAKLLAGAFLDQALNPNTASADAVINFDNAPPTVAVTPA
                     AGQPNPTSGTQIEFTVTFSENVTGFDDPATDLVLGGSALPTSAVITGGPAIYTVTVTG
                     MSVTGTVTSSVPAGAAQDAALLDNAAAPAAASVQWELPVAAQVAFVQQPTDAASGATI
                     APAVTVQLLDAGANPVAIAGTTVTLSLTSGTGTLAGTASQVTNASGLATFADLSIDLA
                     GAKQLTAASAGLTSALSGAFSITVGPVSQLAYVQPPTPAAPGATISPAVTVQLQDAAG
                     NPVAVAGTTVTISLTSGT"
     gene            complement(5579..6544)
                     /gene="moaA"
                     /locus_tag="PLP50_04565"
     CDS             complement(5579..6544)
                     /gene="moaA"
                     /locus_tag="PLP50_04565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPA50856.1"
                     /translation="MDHPRATGAATLRVSVTDRCNLRCAYCMAEVGVPLVPRGEIPRL
                     AELAEAVRWLAERFAVDRVKVTGGEPLVRGGLPAFVSAVAAIPGVREVSMTTNGTRLA
                     ASARELAAAGLARVNVSLDTLDPERFRELTRGGRVEEALEGVEAALDAGLTPLKLNSV
                     LRRSSFRDDVPALLSLAAEKGVEVRFIELMQTGTEAGWAIAERVTGAAVREWLVAGGA
                     RIAPLPDRPSAPARRAAVEWNGWSVETGWIDPVSHAFCGDCDRLRLDARGRLRRCLMD
                     EHAFPLVDLLRRGESDVAAAASLYVCGKAPPDDMTTGSSMASIGG"
     gene            6925..9081
                     /locus_tag="PLP50_04570"
     CDS             6925..9081
                     /locus_tag="PLP50_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA50857.1"
                     /translation="MPEGAFLALVHNASLLLAMALVYDLATTRWWGDESRLRQVSVGV
                     AIGGIGIALMLSSWALEPQVIFDTRSILLGVSGLFFGTIPTVIAMAMTAALRIWQGGV
                     AARMGVAVILTAGGIGLLWRYRRRAALADLGAKELYALGVAVHVVMLAMTATLPRELG
                     FRILAGIGLPVLVIYPAATAALGLLMVNRLRRKRDADQVQESEERTRAALYGVGDALI
                     ATDERTCVMRLNGAAEALTGWREPDAVGRPVREVLRLLSEGPGAEEVDPVTDVMRRGE
                     LRMIGEGTVLVSRESVTRPVLGSVAPIHGPDGSVAGAVVIVRDQTAERARKAAARERE
                     SVFQALFDQRSIGVAHVETSTGRFLRVNERFAEIVGYPREELGDLSFDSLTEPGDFVR
                     DRETVLAILEGRRPNESIDVRYVRKDGSYVWVTASISPIWAPGEEPSTHILMALDVTE
                     RKGAEARMADLNAELERRVARRTELLTEANRELEAFSYSVSHDLRAPLRAIDGFSRIL
                     SEEKGRLLDDEGRRLLGVVSGNARKMGQLIDDLLAFSRSSRAEMTKTRVDMAELARTA
                     FAEARQASPSADRVELRMGELPPAVGDAALLRQVWANLVGNAVKFSSGVERPLVEIEG
                     EVEGRRVVYRVRDNGAGFDMAYAHKLFGVFQRLHATSEFAGTGVGLALVKRIVARHGG
                     DVAAEGAVGSGATLSFWLPLAGPSSGNDLVRDTVAP"
     gene            9164..10984
                     /locus_tag="PLP50_04575"
     CDS             9164..10984
                     /locus_tag="PLP50_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50858.1"
                     /translation="MALLGRYLRPHRALVALTLLLAGVAQVLTLVDPLIFGRIVDDYV
                     LAPGGRPEAELVRGALFWLLVAAAVALAARLASALQEYVMRLLVQSVGRAMFDDGLRR
                     TLRLAFQEMEERSSGETMALLTKVRTDTERFVNASVTVLYSSLVGVGFLVWYGVTRSW
                     LLIPAFGIGVLVLGSLTGLLSRRIKTVQRQVVRETTRMSGAITESLRNIELVKSLGLT
                     RAEFRRLSGYTQRIFDLEMAKVRRVRGLAFLQAAIINVLRQSILFILLWLIFRKALSP
                     GELIAFQFILNTIFGPLQQMGNVLLNHREAEASLAAFGKLMATPVEERPEEPVDVGEI
                     ESLRFENVTFRYRGAAEDALDGIGFEAKLGDTVAFVGPSGSGKSTLVKLLVGLYRPTS
                     GVITIDGVPSNELRYNRVRRQIGFVTQDPQLFSGTIRENLALVKEDATDEEMLEALRR
                     AAATLLLERSGLGLDTRLGESGLRVSGGERQRLSIARALLRRPRLLIFDEATSALDSI
                     TEQEIAASVRDVSGSRQHIVILIAHRLSTIAHAETIHVLERGRIVETGSHDELVEKRG
                     LYYAMWRQQIGERGPWGAPRREAPAPEEAGVADDVLVPEA"
     gene            11081..15157
                     /locus_tag="PLP50_04580"
     CDS             11081..15157
                     /locus_tag="PLP50_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPA50859.1"
                     /translation="MSRRLATLLLSVVPLLPALARSEDAPRAAEPWLDTPLEAPVADV
                     RAAAEKGWPHGSASTRLQTLLVEDRHAFDGEGKRTLTRRRVHKVLTDAGVKDAATVEA
                     YWKPWRDEPPLLRARVIRKDGTALRLDPATANDKPVSEDEDVFDDRKLRQAPLPGVES
                     GAVVETEVVTAEKTPLPGGTRYFSPLGWREPARRYRIVVESPDALPITFKVRGIRDLA
                     PHVERKDGVTVRTWDVVEPEPFVEMDDGPLPSDVETNARLEIATGRSWADVAATYSRI
                     VDGRIAKGPKVPLPAGLPADPRAKVQAVLDALRRDVRYTSLAFGEADIVPAAPSDSLA
                     RHYGDCKDQATLLVSQLRQLGFPAHVALLDSGPGREISAELPALGGFDHAIVHVADPE
                     LWIDPTSLYSRAGDLPLGDRDRWALVCREGVESLSRTPRLRAADSIERVELTVMLADE
                     GRCDVRELSTYAGWVEERLRASWDGTEEKRFREHMEKYATNALGKATLTRAERGEARD
                     LAKRFQLLLEAKGSAFATTGEGSAAVQVSRGFGLGSLPKTTEKKRRMDLAVTPFTREI
                     VVRVVPPRGFEVVETPPSQEEVLGPATLAIGSSVDGEGVASVTLRLVLDRNRLTPDEV
                     AELHSAVEKVSAGGLLSVRLLHASEIDLAEGRIAEAFAKLRQELRDSRESGVAHRRLS
                     SALLRVGLGDAARREAREAVRKSPASPAAQRALARALGHDSFGRAYRPGWDPDGVARA
                     LAEVGKLDPEDLTVLADEAEALEFDAAGHRYADAGRLRAAIGVYEKLLAKAPRATALV
                     RALSTALLFAGRAREVTSLVNGRSDSAALSDVAIAALAVSDGAQRAREEGRRQLAASE
                     RSAGFEKSAELLVSLRRYPEAAELYREAARSSEKAAALGARADFSARISSTPPALDGK
                     DAESVLRAFLYHAYAPRKPAPEELHPFAARALRPFIPEFVDALRSVSLSSGFASRELA
                     RDVSTFGPEWFSQGSPESGVRMRAIGNGPEARDNWIAEEEGRLRVLSIGIPDPLCRHA
                     ETLLGKGDVAGARTWLQRAREMDLEESREGPPLDRPVTRWFDPASEDPSELLLSAIAC
                     QQVELLGDDALEKLDRLAAERPEDDRRKALLVKAEFAMGDAARALEIARDLYRRNPDS
                     ETAATALLLALQENGHSAEAIRTAQAQLEKWPRQLDVRRVLSELRARAGDFEGAAREG
                     EELLGLPRSNALDLNNAAWHRMVAGQLDEKTLSLAQRAVEGTGRLEATTLNTLGTVYA
                     EMGRPSEARAVVLEAMDLAESAEPDPSDWYIVGRLLESFGLISDAREAYGKALVVPSS
                     DASKPRRRAEREARDLPGSPSWLATRRLAALGEAPAGR"
     gene            15495..18686
                     /locus_tag="PLP50_04585"
     CDS             15495..18686
                     /locus_tag="PLP50_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HPA50860.1"
                     /translation="MIGSAPAQAQPSTDRVPTLAEQLKKAGARPGTAFERLIKENQDF
                     SVLRADEKADTGRVPPWLKVIWRKANPDGHYTAADPTGGYPFVLKEVLEWMQSHQDLR
                     PGLPDANFEAGYDNPPDGVGTWDEDLSVTALATSIGTNYRISGSQSSPRSESDIRINY
                     WNPNQIIGASNNISGSGFQAQFYSLDGGTTWGQTTLPGYSGDAFNSDPTVDWSSDGTA
                     WSSTMGINSTGTILKIRMYKSTDQGRTWTYDATISGSQTNTDKQMVWIDHSATSPYKD
                     NIYAIWHNGNPVYMNRRNSTGWLSTPIQVSGSQTTGTGIGSDVKTNSYGDVFGLWPDT
                     GSRGLYVVKSTNGGTSYGTPVKLATTYDSYDIGVPAFNGRRILIYVTAGAYRTASKNL
                     VYALWTDLSGETGCTTASNEPGSSVTSACKTRIWFARSTNGGTSWGTPVKINNQSGKN
                     DQFNPWLVVDETNGAIAVMYYDTVDDANRKKTHVYYQSSFDDGATWGAPVKVTTAQTD
                     ETGSGADSGNQYGDYNGLSGYAGVFFPSWTDRRNAASEEIWTAKVTDSGSGATYSLSG
                     TITLSGSGLSGVTVTAGSGSATTSTSGAFTISGLAPGSYTVTPSRSGYTFSPASQSAT
                     ITSSNVTGVNFTATAVSTTYSISGNVGTAGATVTAGSASATSDASSNYSLATLVAGTY
                     TVTPTKTGCTFSPASQSVTVTSANVTGVNFTASCPSGDLQLTSGVPVTGESVAKSAWK
                     YYSIVVPSGATNLTFATTSATADVDIYTQNGAKPTSSSYICRPYSSSGNETCSATNPA
                     AATWWLGVYGYAAGSYTVTGTYTLGGTTYTVSGSAGTASATVTAGSASATSDASGNFT
                     LTGLAAGTYTVTPTKSGCTFSPTSRSVTVSSANVTGVNFTASCGGGETTLFNDGFEST
                     GWSQVDTSGTAGSWTFATSSTYPTTSPHGGTKLAKFNSYNASSGSQTRVYRSSGFAVA
                     SSYTSVTLKFWMYHDTGYSSRNDRIQPQVSVNGTTWTNVGSAISRYSGSTGWAQVTID
                     LSAYKGQTVRLGFLGISAYGNNMTLDDVTVTAQ"
     gene            complement(18783..21173)
                     /locus_tag="PLP50_04590"
     CDS             complement(18783..21173)
                     /locus_tag="PLP50_04590"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPA50861.1"
                     /translation="MNVRVLAALLAAASPLSAAPTPSLAAARLAGPLTVDGRLDEAVW
                     TQAAAASDFRQWSPNRGEPARLETRVRVLFDDAYLYVGASLENPGGPAAIAARVHRRD
                     RDSYSDWFGVSIDVEGRGRNARMFRVNAANAQRDLLQFEEAADDDTWDAVWDSATSMG
                     ASGWSVEIRIPLEVLRPKASPAPQRWRIYFYRENQSPYEVTGWMVVPRGAQGFVSHFY
                     PLEEVVVAKPPRRRDLSAHASARSKVRTAEPYDDLGEAFRAGADASLGIGATGQLDVS
                     LLPDFGHVEVDRQVLNLSTTETFFPEKRPFFTEGADAFTLPGLQLFYSRRIGHALAPP
                     AAPEGTTVLERPLNVDLLGAARYSRHSDDGWRMAALGAVTQRAEAELRDRDGRIETAT
                     LEEAQAYGVARVSRALGDRGGYVGAFVGWTGQRGANGREAQVAAVDGSYRSASRRTKL
                     TGILAGSIAGSGENDRRGWGADLELVHTFRTPWTATVHLTELSPQFDPNDLGYLARPD
                     VRQGWVDIRRQWDCTAGVFRNWEIAFGGMVRRDHAGHTLTREVEATLGTQFTNDWRVS
                     AIAGAYLSADDDQELRTFGQERKLILRRPDRPFAILSFGTAPQAAWSVSGEARWDSWE
                     GGPTNELAVSQIVRPTSRLEVELDTSYQRASGEERWLETPTPGAPVVGLRALRQLDQS
                     IRISFAVSTRATVQLFSQWRAARWSFRETRAWNDAGSRVAAAAAGPTTFSDRTWALNL
                     IGQWQFRRGSTAYLVYTKGAAANERGGAGLGLSDDLASLHHVPSQDVVQAKVSWRF"
     gene            21299..22441
                     /locus_tag="PLP50_04595"
     CDS             21299..22441
                     /locus_tag="PLP50_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPA50862.1"
                     /translation="MTLRPELDRWMRRLTLLMWGAVGWAMLAENLHREGTVPVACLLS
                     WAAAYLLCGLSFLLASSEGIHRWPRWTWVVILLAQTGSVLFMTGWWRSLAGGFLLVFS
                     AWQAARFLSFGASLAWVVSQSVLFGLILVSSDPSAPWLVVAVFCGGLQLFAFLVAHLV
                     RQEEAARLGLARSNSELRDAHELLARVSRGSERLRVARELHDGLGQKLVALNLALESA
                     ERDLSRPVDGLARARKLVHGALAELRVAVGRLREEPGPDLESGLRELVADIERPRVHL
                     EVRGTCSRVDAARARALLRCAQELLTNAIKHSGAENAWLLLECTADGERLVVRDDGRS
                     DGVVLEGCGLKGIRERIGALGGTLALGARPPGLVASVTIPAFGEGP"
     gene            22438..23079
                     /locus_tag="PLP50_04600"
     CDS             22438..23079
                     /locus_tag="PLP50_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002807809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA50863.1"
                     /translation="MIRVLVVDDQTLVREGLVALLGSLADLEVCGEAADGEEALRILE
                     ESAPDVVLLDLRMPRLDGLGVLEALSARPAAPPVLVLTTFDDDAALLEAVRLGARGYL
                     LKDVRLDQLADAVRTVAAGGELIHPSLSSRLVETLAGRPGRFDALDLPDPLTRREKAV
                     LRLAAAGCANEEIARALGLAVGTVKNHVSTILSKLGVRDRTRAVLRAYELGVL"
     gene            complement(23296..24294)
                     /locus_tag="PLP50_04605"
     CDS             complement(23296..24294)
                     /locus_tag="PLP50_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50864.1"
                     /translation="MTWTASVLPLPRGFFGAGSAGLCGGAVVGNVEAAQGGSRVCLWD
                     GEGPRVVDTGALRLQARSARQTTVVGAVRRRSGGQAALLRFGPEGEARVVSLHPRGFL
                     ESVAAATDGDTRVGSGRTATSGAALLWPGTGAAPTILPDPGGEGARALGVRDGLVAGA
                     AGWPSRAVLWRGTGEPVSLHPQEAGWSEALGVGDGEQVGLIARTEEAFLAGNVLPVVW
                     RGSAATMTKLLPGGCTAGRAVDVLEGVQVGSVRGGGVERAALWSGTAASHVDLHGLVG
                     GDWTDSAAQSIHRREGALCVLGEVRRMAPDRRGIAARQVVVWTSTSPSREPRSPRS"
     gene            complement(24411..24842)
                     /locus_tag="PLP50_04610"
     CDS             complement(24411..24842)
                     /locus_tag="PLP50_04610"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPA50865.1"
                     /translation="MEKARVLVLCTSNSCRSQMGEGWVRHLLGERVEVHSAGTRASFV
                     HPRAIQVMAEAGVDLSSHASKSVEALQGLPFDLVVTVCDSAREACPVFPGARKTVHHA
                     FEDPAHAGEGDDALPVFRRVRDEIREALPALVARELGFPEG"
     gene            complement(24844..25920)
                     /gene="arsB"
                     /locus_tag="PLP50_04615"
     CDS             complement(24844..25920)
                     /gene="arsB"
                     /locus_tag="PLP50_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPA50866.1"
                     /translation="MPDGVAKKLSFLDRWLTFWIFAAMAAGVLAGAFIPGVESFVNSF
                     QVGTTNIPIAIGLILMMYPPFAKVRYEEMGDVFRDRKVVALSLFQNWIVGPVLMFVLA
                     IVFLRDRPEYMAGLIMIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSVFQVLFYSVY
                     AWFFITVLPPLLGLKGRLVDVSMAEIAKSVFIYLGIPFLAGALTRFVGVKLKGKEWYH
                     RRFVPAISPITLIALLFTIVVMFSLKGKLIVTIPLDVVRIAIPLVVYFVLMFVVSFWM
                     GKKVGADYNKTTTLAFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLVEVPVMIGL
                     VNVAFWFQRRYFGAPSARAAAQGA"
     gene            complement(25975..26820)
                     /locus_tag="PLP50_04620"
     CDS             complement(25975..26820)
                     /locus_tag="PLP50_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPA50867.1"
                     /translation="MNDNEIRDVVREKYGKAALQVKEGRTSCCGPAPSACCGTAPSAS
                     GLSGDPITSNLYADHETALLPTTAVQASLGCGNPTALAKLEEGQVVLDLGSGGGIDVL
                     LSAKRVGPTGKAYGLDMTDEMLALARENQKKAGATNVEFLRGTMEEIPLPDGSVDVII
                     SNCVINLAADKDRVLREAFRVLKPGGRFAVSDVVTKGEIPAPVKKSVELWIGCIAGAL
                     DEDDYVAKLTAAGFTDVTVEPTRIYRVDDAREFLSSAGIDVDALGPEVDGKFRSAFVR
                     AVKPA"
     gene            complement(26873..27238)
                     /locus_tag="PLP50_04625"
     CDS             complement(26873..27238)
                     /locus_tag="PLP50_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPA50868.1"
                     /translation="MATTTSRGKPVRTGLPLAREDRAERLADLFHALSDPTRIRILAA
                     LRNGEQCVCNLTGLLVAQQSRLSFHMKTLKDAGLVKDRREGRWIHYSLVPEAVEEIRV
                     ALEELEAAAKSCAASCCRT"
     gene            complement(27366..27770)
                     /locus_tag="PLP50_04630"
     CDS             complement(27366..27770)
                     /locus_tag="PLP50_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPA50869.1"
                     /translation="MSDGRDTCVVDLVAEPFAAIEPPEEALIEGAAISVDGRREILAS
                     KLATLLERSEVRDLPDVKTLLDAGGDLGAALRDAPKRDAGFSPLTLVWMLKDYDPRPA
                     VKALGRDARQADELLSLRQWLIEQLTARAAPE"
     gene            complement(27872..28174)
                     /locus_tag="PLP50_04635"
     CDS             complement(27872..28174)
                     /locus_tag="PLP50_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50870.1"
                     /translation="MLSFTPRERLTDAQDRPDFLWDGDLTLSQFREGLADPDPEVHAY
                     LIGKLMRQAKPDDVFLFVGPGEIRDLWPRLTRCLGRTREFLSWLFETWEGQGRVWQ"
     gene            28252..30963
                     /locus_tag="PLP50_04640"
     CDS             28252..30963
                     /locus_tag="PLP50_04640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA50871.1"
                     /translation="MSAPGDSPEPAHVILALAVTPHGHLRLDRTAGDADDPTAGVAAR
                     IEAAFARGVGAGVLHLGAAEPGTHLPPSLAFFRDFGQLFVTALSRLPDAEGRRAALDL
                     PVPADELDARVAAAPPLDGLEYLTRGVLETLWSDLNATARRELAEWKGDVESWLQARS
                     PLWHSVGRVCFHLAENKTSETKPFAFLATYATRLSEAGRVQHQPLGHAVKTSGAARDR
                     DALRSLLVPVQRAAEKSPFVKALVDSGQVFQPLAWTSSQAWSFLREAPLLEESGVLLR
                     LPDWWKKGAPPRPEVRVTVGEALVSGVGGDSLLDFSVELTLGGEALTAAERRQILAST
                     AGLALVRGRWVEIDPAELKEALARLAKAAESQGEGVGIGEALRLAAGLGTSPGGGGDG
                     GWVRVEPGRWLAKALESLRTPEGRAAADPGPALLGTLRPYQKDGVAWLHTLSRLRLGG
                     CLADDMGLGKTIQVLALLLALKRAGEKGPHLLVVPASLVANWTAEIARFAPSLSVLVA
                     HPSVTPAKELADAATAAGRVDVTITTYATLLRLPALQEVRWGLVVLDEAQAIKNPSAK
                     QTRAVKALASRGRLALTGTPVENRLGDLWSLFDFLQPGLLGSAKEFGQFTKARATSAN
                     PDWGPLRRLVRPFILRRLKTDRSVISDLPEKTEMKVFCPLSKTQAVLYQQAVGELAKL
                     LSAVDGMKRRGVVLSYLMRFKQLCNHPSQWLRDGRFDPKESGKLGRLAALGEEIASRQ
                     EKALVFTQFQEMTGPLASFLAGVFGRPGLVLHGGTPVAKRRALVEEFQREDGPPFFVV
                     SVKAGGTGLNLTEASHVVHFDRWWNPAVEDQATDRAFRIGQKRNVLVHKLVCRGTVEE
                     RIDALIEAKKALARDVLEGGAEARLTELSDAELLQLVSLDLAAATAE"
     gene            30972..32033
                     /locus_tag="PLP50_04645"
     CDS             30972..32033
                     /locus_tag="PLP50_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPA50872.1"
                     /translation="MAYGYGYGWGYAPYVSVASRRARAAKQAASLRKKGQILEPVTIE
                     GRTVARSFWGKAWCRNLEVYGDFSNRLPRGRSYLANGLVLDLKVAPGLVTALVQGSDL
                     YEIEITVDRLAEAKWRAIRSECAGKVDSLVELLDGRLSQAVMSVVTRPGAGLFPSPAE
                     IRMRCSCPDSARLCKHLAAALYGIGTRLDHRPELLFLLRQADHLELVTAAVDAPIAGP
                     ASRDEALAGADLSSIFGIELEEATSEAKAPARARKPRSTATPRETPEAAAVLAAVPKL
                     KRGKPAGARLAKMAKVAKVAKEMTTADLVALGVSRATVRNWLAERVLEPTDRRGVYAR
                     TSETTRRIKAYLSRRRKGS"
     gene            complement(32252..32677)
                     /locus_tag="PLP50_04650"
     CDS             complement(32252..32677)
                     /locus_tag="PLP50_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA50873.1"
                     /translation="MTAPRIVLDASAALRVVLASPGIEEALAALERASLVLAPSLFAS
                     ETANALWKLGSKGLLPAEVLAERWEMAMALPSRLCPDHELAVEALSEASRHGHPVYDL
                     LYAVLARREGCRVLTFDRRLLSLLRKLGIGVIGGASAGA"
     gene            complement(32674..32913)
                     /locus_tag="PLP50_04655"
     CDS             complement(32674..32913)
                     /locus_tag="PLP50_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50874.1"
                     /translation="MSSLQIRELPAELYEALALRARLEQRSLAQQAVTELRKTLSDGS
                     ARKLVLADVRRRLREEKPRPPRVSLEKLVREDRAR"
     gene            complement(33045..33734)
                     /locus_tag="PLP50_04660"
     CDS             complement(33045..33734)
                     /locus_tag="PLP50_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPA50875.1"
                     /translation="MTPDLAPAIATALWLGVLTSISPCPLATNIAAVSYLARRADRPR
                     LVLLSAFTYSLGRALAYAVVSAVVVLGLLSVPGLSQFLQRYMNKLLGPLLVLVGIYLL
                     GLLSAGLSTTAGSEELRTKAADGGAWGAALLGALFALSFCPVSAALFFGSLVPLSLQQ
                     GSKVVLPVVYGFGTALPVVVLALVATGGTKALGAAMTKVGRFEVWARRITGVVFVAVG
                     IYLSVRFVFLG"
     gene            complement(33731..34225)
                     /locus_tag="PLP50_04665"
     CDS             complement(33731..34225)
                     /locus_tag="PLP50_04665"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPA50876.1"
                     /translation="MSIGKKHLVTGALLAFVAVTGITLVVKETRHARAVEASAAAPAS
                     IPVPPAAEASTVVVTYFHTTARCLSCLKIEELTNATLTMRFADPIAEKRVVWRLVNTD
                     EPANAHYMKDYGLFTKSVVVSEVKAGKELRWKNLDQVWPLLRDPEAFQDYVEREVRAY
                     LEKA"
     gene            complement(34222..35409)
                     /locus_tag="PLP50_04670"
     CDS             complement(34222..35409)
                     /locus_tag="PLP50_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPA50877.1"
                     /translation="MKERTKLLLIVGVFLGAWFLPIESVRVQRALPEGFVLLRDYARE
                     HVLLCLVPAFFIAGAIGQFVSQGAVMKYLGATAKRPVAYGVGSVSGAILAVCSCTVLP
                     LFSSIYKRGAGLGPATAFLYSGPAINVLAMILTTRVLGLQIGLARALGAVAFAVVIGL
                     IMAAIFRKEEAERVKNGPDPFAAAGAAAEGRTREGWQDASFLGTMVAILVFANWGASN
                     RAVGFFAAVYEVHWYLAGAALAALLFMMIRWYDKEELRLWVLGTWEFAKQILPLLLGG
                     VLVAGWLMGRPGHDSGLMPDAWIAGAVGGNSLAANLFASVAGAFMYFATLTEIPILQG
                     LLGSGMGKGPALALLLAGPALSLPSMIVIHSYLGTKKTVAYVSLVIVMSTVTGLVFGA
                     FAS"
     gene            complement(35465..35716)
                     /locus_tag="PLP50_04675"
     CDS             complement(35465..35716)
                     /locus_tag="PLP50_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH895/ArsE family thioredoxin-like protein"
                     /protein_id="HPA50878.1"
                     /translation="MESAPRKIEVLGPGCARCKETYRVVAHVVETEKLPFTVQKVEGM
                     ERMIELGLLATPGVAVDGKVLFSGRIPKAEELRAALAAL"
     gene            35945..36967
                     /locus_tag="PLP50_04680"
     CDS             35945..36967
                     /locus_tag="PLP50_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA50879.1"
                     /translation="MNRRTFLAALAALPAARLLGGSAAATAREMRVFGTLPRPDRVRR
                     VFAAGPPAAVLAHVLSPERLIGWPMPLPPEARALLAPAVRERPFLGRLSGRGSTIPLE
                     KLLALEPDLILDTGTVDATYLSAAENVHERTGIPYLLVPGRLSDSPWQLSETGRLLGV
                     PERGAHLAREAERFLAEARGSRRDGVRAPGVYVARGADGLETVKEGSLNAESLALAGG
                     RSVATGLGGGVARVSFEQVAAWAPEVVLTQDAGFFASARRDDRWRRLPAVRAGRLHLV
                     PSLPFGWLDGPPGVNRLVGLPWLASRLHGTPLGKDEVERVRAFHAAFYGFAPERTALE
                     RLLAGA"
     gene            36967..37953
                     /locus_tag="PLP50_04685"
     CDS             36967..37953
                     /locus_tag="PLP50_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPA50880.1"
                     /translation="MPRWPWLLLGLLLALAILSGLAAGPIALGPGELFRALLGGPSAE
                     GQATMVVWSLRLPRLAAALLVGAALSAAGTAYQAMFRNPLVSPDILGASAGAGMGAAL
                     AILLGLPTLGIQGAAFAGGLAAVGLVTLVAGRVRRHDPVLVLVLAGIAIGTLFSAGIS
                     LAKVLADPTVQLPSITFWLLGGLSASSPADVWRAAPLVGAGLLPLLLLRHRVDLLTLA
                     DEEAAALGEDVSRLRFTLIVSATLVTGASVAIAGIVGWVGLVVPHAARLLVGPRFARS
                     LPAALLLGAAFVAIADTLARSAAAIELPLGILTALVGAPAFLWLLARGGRFS"
     gene            37950..38714
                     /locus_tag="PLP50_04690"
     CDS             37950..38714
                     /locus_tag="PLP50_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50881.1"
                     /translation="MTKLEARGLSFGYDGTPVGEGVDLDLTGGEVVCLLGPNGGGKTT
                     LFRTLLGVLPPIAGEVRVAERPLSRWPRRERARLLGWVPQAHAGLFAFTVEEVVLMGR
                     TARHGRYTAPSRRDHDVARAALERLGIGRLAERVYTQISGGERQLTLIARALAQEAGI
                     LILDEPTASLDFGNQLRVLGEIRRLRDDDIGILMSTHQPEHALEVADRIALLDGGRIV
                     AQGPPRETATPERLAHLYRADLAAVTARIAPSGARP"
     gene            38768..39256
                     /locus_tag="PLP50_04695"
     CDS             38768..39256
                     /locus_tag="PLP50_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPA50882.1"
                     /translation="MGSLALLTGGRGAGKTRWCEALARAAREAKLAVRGVLSPPVLAG
                     GEKRAIDLLDLATGSRRFLAERARPDLPGTEGLGWRFDPEALAAGNVALERVGACDLL
                     LVDELGPLELGRGSGFTAAFGLLDARRHGLAIVVVRPSLVAAAGARWPGATRLWDLGG
                     DR"
     gene            39253..40071
                     /locus_tag="PLP50_04700"
     CDS             39253..40071
                     /locus_tag="PLP50_04700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA50883.1"
                     /translation="MTDVDGKRPETILDVDFGRLYRQHFAASGGREKPPEAWDARASD
                     LSRELEGSPYVEELLRLVDLTDCRTLLDVGCGPGTIALALAPRLSKVWGLDFSRGMLD
                     AMTRHAAARGLANVEPILRSWEEPWDDVPACDLVLASRSTLVRDMEDALARLDAKARR
                     RVCLTSLAGGRFVRADLLALLGREKPAYPDYIYVVNLLHRMGRHPRLDFIRAGRRPDE
                     KPGFDQLARRVEFSLGALTPEERGRLEAWYREDPARAGTGREPSRWAFVSWEKE"
     gene            40367..43564
                     /locus_tag="PLP50_04705"
     CDS             40367..43564
                     /locus_tag="PLP50_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA50884.1"
                     /translation="MAPSTALLFLVLGGVAAVAARPGRGAGARSAGVVLASLAALGSA
                     VLLVARLRGTYLSIEHVGLRIEGRVSGWPLGHISPVAAAAFFLVSVSLLALQIPPGRG
                     RTRGWLATAAGLVVLVCGVVFFLAYVVGEPLLYRGPVLPPAVTTSTALVTIGLAVVLA
                     ARARIPRPDDEVRLPRSAEAMLAGLFLVFAAGIVLWGATWFREQGRRLRERVDLQVST
                     IAELKATDLARWRTERLGDGRVLQESRAFRRLVDGLLVGRLTSDDEAVLGQWLGSVRE
                     SYGYASVEVLDARGARRLSIPNDAAPIVGDDGRHFAELLRSGEARLEDLHTHEPRGVL
                     RMAVVVPIVLEGRPRGAVLLGIDVRSHLETFLSDWPVPSQTAATFLVRQDGDAVLVAR
                     AADADGKEPRIEPVPKAQVEPMFVRAAAGEEVVGLFPRPDGSQVVAAARRVTGSPWKL
                     VASVDVEEAYGPLRERLWLTVLAMAGLLAAVAAGVLAVWRRQLGRAERGRRLAEREGA
                     SLREVIERSLNEIFVFDPVTFRFSFANRGAARNLGYSEEELRGMTPMDLVPGMTPDDF
                     GAMFRELDESPGEVYRLETVHRRKDGSVYPIEVNLQRVSAAAGDVYLAVINDITERKA
                     AEGRIRHLNRVYAVLSDVNQAIVRERDIPSLAAEACRIAVSVGGFHDARLFLAEDGAI
                     RSVGRAPAAEPDPSAGESAWAETLALESIRQGRSATSRAGETTDAAASGAARDRGPAV
                     ALPLVVEGRPAGAFVLAAGPDDHLDVEEMKLLEEMALDVAFALETIRGEAARKQAEAE
                     VRRLNADLERRVEERTAELASAVRELETFAYSVSHDLKAPLRAIDGYSHLLVEEFGRA
                     LDGKARDYLERVRGGARRMGELIDALLAYSRVERKALQPESITLGESVRLALAHVQEE
                     VASTGASVSVDVDGLVVRADREGLAIVFRNLLGNALKFRRGAEAPRIEIGGESSGGTC
                     RFWVRDHGIGFDTVYGEKIFEIFQRLHRQEEFPGTGIGLALVRKAVERMGGSIRAESE
                     PGHGATFFVELPGGASGMSPGAGAPGAERTSGSGG"
     gene            complement(43561..44883)
                     /locus_tag="PLP50_04710"
     CDS             complement(43561..44883)
                     /locus_tag="PLP50_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPA50885.1"
                     /translation="MSPMLRRVHGIALAGLLLLVGAAPAQVNLLWYKEVAKDGRIYVF
                     NDPNEFKAWEGSGELGKSITKIGFGPNGETVVFDNDAALDLYVFKHGKEPEVRPYSAP
                     KPPKPVPPTTLKIGDGELKFSGLLQGWYVMDDSATGSGSSQLGNSTGVNTFRLRRAEI
                     KLSGKVTKEWGFEVMIDPTKSPSVTTGADGKILQDFAISYSGLKGHDLQLGQKKVWLM
                     EEGNRSSSALDFAERSVVARQFADKRELGLFWKANWSPMVTTGVSVTQGTTLNNNDNN
                     DALNFNARLDVKPTKGLVVGASGGYSAGEGAAYLGRERYAAHVRWEGTEELPLGFRFE
                     YAEGRDEQASGGVVTKLKRNGWYGTVLYTFAKKLQLAARYEQFDRNKESATDNKLTIL
                     TGGLHYFIKGNNANLKLNVESIEDDGRSVGGVLDEKYLQAVVAAQVSF"
     gene            45142..46107
                     /locus_tag="PLP50_04715"
     CDS             45142..46107
                     /locus_tag="PLP50_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPA50886.1"
                     /translation="MKMTATLAAAVLLAGTAAAQTIQVDPAIKPYKAVAGVSGNVSSI
                     GSDSLNNLMTLWAEKFNKMYPNVKIQIEGKGSSTAPPALISATAQLGPMSRAMKGSEI
                     DEFEKKFGYKPTQVRVAVDALAVFVNKDNPVACLTMPQVDAAFSKSRRAGYKENVKTW
                     GQLGLTGDWANRPVSLFGRNSASGTYGFFKEHVLKNGDYKDEVKEQPGSASVVQGVTV
                     DRFAMGYSGIGYATAGVKALPLAAKQGDTCHPATPEEAYSGNYPLARFLYVYVNKAPG
                     KPLDPIVGEFLKLVLSRDGQEVVVKDGYFPLPAKIEQEELAKLLK"
     gene            46118..48385
                     /locus_tag="PLP50_04720"
     CDS             46118..48385
                     /locus_tag="PLP50_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPA50887.1"
                     /translation="MPATTESVAAGRPPAPARTTGAARPLGRRLLADRFAARFVTLSG
                     VAIIAAILAILFVIVAVVWPLFVPPSARAVPAGSLPAGTVPLALSVDEYREEAFVATK
                     AGLVGLPVPGGEVFTRELPLLGDATIVKAGAPEPELIVLGLSDGRLYPVEVSTEVAYE
                     DGRRRVTPVVRPGEPVTVTPDGKPVEVFKYVRGPDGPIALAATGPREITLVRVTEKTS
                     LMGETTRETSSLVLPIRARGVIRTLALDVRGQDVFAGTSAGEIVRLDLRDEQSPKQEE
                     TVPASATGREVTVLGFLNGDRTLVSGDAEGRVASWHVVPSETGNTRRLTRIHDFRSHG
                     AAVTAFSPSRRDKGFVTGDAAGGVAVHYATTGKTLLSLSAGTAPLAAVVFAPKADGVL
                     AAAADGRLHHFELKNPHPEVTWRSLFGKVWYEGYARPDYVWQSTGGTDDFEAKFSLTP
                     LIYGTLKGTFYALVLAIPLALAAALYTAQFMHPSLRSFVKPVVEVMAALPSVVLGFIA
                     GLWLAPIVERIVPGLLVMPVVVGAFAALGAVGWGKLPARVRARVPAGWEALLLLPLVV
                     AAGAVSLWIGAGLERWLLGADYRHFLLEALGLTYDQRNSLVVGLAMGFAVIPIIFTVA
                     EDSISAVPPGLNAGSLALGATRWQTALRTVLPTASPGIFSAIMIGFGRAVGETMIVLM
                     ATGNTPVMDLSIFNGFRALSANIAVELPEAPEGGTLFRVLFVAALLLFVMTFVVNTVA
                     ELVRLRLRKKYRTFE"
     gene            48382..50037
                     /gene="pstA"
                     /locus_tag="PLP50_04725"
     CDS             48382..50037
                     /gene="pstA"
                     /locus_tag="PLP50_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPA50888.1"
                     /translation="MSASEGRPSLARKSGVFRSGEPFIWVTGSGLAVAVLMVFGLLWL
                     VLANGLGFFWPADVHLFALEDGRALAGRVAARERIPDPDAAPGTPERWRIKVKQGNRD
                     LTGEDFVWVDESAVRSRTTPADVVVLERREWGDFYGWVSEVRDGETLLASGREASWKK
                     VVELLPRAREVHEEALRLEKGPIGAVNFEKEKLRLERKKLELEGAAAGSKRNGLDAEE
                     RELQAEYDRLFAALQEVRAGVKAVLVLETAGGQTKELPLSQVVRADRVNGMGRLAKAG
                     RYAANVWAFVSEDPRESNTEGGVFPAIFGTVLMVLIMSVVVTPLGVLTAFYLREYAKQ
                     GPVVSAVRIAVNNLAGVPSIVYGVFGLGFFIYFVGGSIDRLFYPEALPAPTWGTGGIL
                     WASLTLSLLTVPVVIVSAEEGLAAIPRGMREASLALGATKFETMWRVVLPAVMPSILT
                     GLILAMARAAGEVAPLMITGVVKLAPSLPVDGFAPFFHLDRKFMHLGFHIYDVGFQSP
                     NVDAARPMVFTTTILLILIVVALNLTAIVIRGRLRRKLRSSGI"
     gene            50049..50891
                     /gene="pstB"
                     /locus_tag="PLP50_04730"
     CDS             50049..50891
                     /gene="pstB"
                     /locus_tag="PLP50_04730"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPA50889.1"
                     /translation="MEAQTMAPPASVPPLELRTAPGPEGAAAEAPPLIELEKFSFWYG
                     EKQALFDIDLKVRERSVTAFIGPSGCGKSTLLRCLNRMNDLIDGVRQEGSLRVAGQDL
                     YAPDVDVVSVRKRLGMVFQKSNPFPKSIFENVVYGLRIQGERNKTVLRDACESALRGA
                     ALWDEVKDRLDESALALSGGQMQRLCIARALAVEPEVVLMDEPCSALDPIATAKIEEL
                     IFALKERFTIVIVTHNMQQAARVSDETGFFMLGKLVEFDRTDVIFTRPKEKMTEDYIT
                     GRFG"
BASE COUNT         7116 a        18076 c        18335 g         7382 t
ORIGIN      
        1 tcctcttcct cttctacggc gacctgccgc tgaagggcga cgccgtcttc ccgaaattca
       61 tcgtcgagca ggtcccgtcc gggctctcgg gcctcatcat cgcggggctc ctcgccgcgg
      121 cgatgtcgac cgtctccggc tctatcagct ccatctcgag ctccacggtc ttcgacctct
      181 gggcgtcgac gaagcgggga cgtctcgcga gcgaggagga gaagctccgc ctgtcgcggc
      241 gcgtcagctt cggctgggcg atcctcctga ccggggccgc gctcgtcttc atcggcacgg
      301 gacaggccgt cgtcgagctc gccctgagca tcgcctcctt cacgtacggc agcttcctcg
      361 gcctcttcct cctcgccgtc cgcgggcgga cgagccggcg cgcggcggcc gccgggttcc
      421 tcgccggggt cttcgtgatg gtcttcgtcg tcttcgggac gcccctcgcg tggccgctct
      481 acacgctcgt cgggagcctc gcgacgcttc tcgtcgccct cgccgtcgat cgtctccggc
      541 ccgagcgtcc ggccgtcgga gaggccggcg cgtgagccgg ggcagcctcg cgttctgcgg
      601 cgcgctcgcc gcggtcgccc ttctcctcgc cgtcccgtcc tcgcaggcgt gggtcgtcga
      661 gacgacccgg gcgcacccgt acgcgatggg cttcctgaag ttcgcgatcc tcgcgagcct
      721 cggggagctc ctcgcgatcc gggtcgtctc gggcggctgg aagctccccg cggggccggc
      781 gtggcgggcc gtcgtctggg ggctcctcgg cgcggcgctc gcgctcctct tcccggtcta
      841 cgccgccggg gtcccggcgg cgatcggggc cgggctcctc ccggtccttc cgggagcgct
      901 cggcgcgaag gtggcgacgg cctgctgggt cagcgcggtc atgaacctga gctggggcgt
      961 cgcgctgatg ctccttcacc gcctgacgga cacgtggctc gatctcgcgg acggggcgct
     1021 cgcgcgggtc ccgtccgtcg gggtcggcgc ggtggccgcg gcaatcgact ggaaggggtt
     1081 cctcggcttc gtcctcgcga agacgatccc gctcttctgg atcccggcgc acaccgtcac
     1141 gttcctgttg ccgccggagc tgcgcgtcct cttcgcggcg ttcctctcgc tcgcgctcgg
     1201 cgcgatcctc gcgttcgcca agcgccgcgc cgccgccggc tgaagctcga cccggcccca
     1261 tcggcgggcc ttccggatgc ggccggggcc gggcggcagg agacgttcac gagcccggga
     1321 gccccggcgc agcgtcgtca aatgcgcgac cggtggacga gatcgccacg ccccgaaggc
     1381 gcgaagggaa ccgggcgaat ccggttccct ccgcggcctc cgtccgcgat cagaccttga
     1441 acacgcccct cgacgccagg gcgccgacag cggcgagcag gagagcgaag acgccgatgc
     1501 cgaagccgcc gagaacgggg acattcgcga tggcgtccgc cgagaggttc gtgagcgcga
     1561 acgcggccgc cggcagcgcg ccgatgctgg ccgtcacgat gtacggcccg cccgggatgc
     1621 cgttcgccgt cgcggtgacc caggcctgcc cggccgcgtt cgtcgtggcg atccctccgt
     1681 tgctcaggag agcgctcggc ccggagatcg gagcgaggaa ggtgacggtc actccgctca
     1741 cggggttccc accggcgtcg gtcaccgtca ccaggagcgg tgccaggaat gctccgtcgg
     1801 ccggagcgct ctgaagcgat ccggcggtca ccgcgatcgc ggccgccgga ccggcggtga
     1861 tcgtgaaggc gccggagagg gcggaggtca ggccggcgct ggcggcggtg agctgcttgg
     1921 cgccggcgag gtcgatcgag aggtcggcga aggtggcgag gcccgaggcg ttcgtgacct
     1981 ggctcgcggt gccggcgagc gtgccggtgc cgctggtgag cgagagggtg accgtcgtgc
     2041 cggcgatggc gacggggttg gcgccggcgt ccaggagctg caccgtcacg gccggggtga
     2101 tcgtggcgcc ggaggcggcg tccgtcggct gctggacgaa ggcgacctgg gtggcagccg
     2161 ccgccgtgat cgagaaggtg ctggagacgg cggaggtcag gccggcgctg gcggcggtga
     2221 gctgcttggg gccggaggtg tcgatcgaga ggtcggcgaa ggtggcgagg cccgcagcgt
     2281 ccgtgagctg gctcgcggtg cctgagagga tgccggtgcc gttcgtgagg ctgatcgtga
     2341 tcgtcgtccc ggcgacggcg acggggttcc cggcggcgtc ccgcagctgg actgtcaccg
     2401 cgggcgtgat cgtcgccccg ggagccgcgg gcgtcggcgg ctggatgtag acgagctggc
     2461 tggccggacc ggcggtgatc gtgaaggcgc cggagagggc gnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn ncggtgccgc tcgtgaggct gatcgtgacc gtcgtcccgg
     2641 cgacggcgac ggggttcccg gcggcgtcct gcagctggac cgtcaccgcg ggcgagatcg
     2701 tcgccccggg agccgcgggc gtcggcggct ggacgtaggc gagctggctg accgggccga
     2761 cggtgatcga gaaggcgccg gagagggcgg aggtcaggcc ggcgctggcg gcggtgagct
     2821 gcttggcgcc ggcgaggtcg atcgagaggt cggcgaaggt ggcgaggccc gaggcgttcg
     2881 tgacctggct cgcggtgccg gcgagcgtgc cggtgccgct ggtgagcgag agggtgaccg
     2941 tcgtgccggc gatggcgacg gggttggcgc cggcgtccag gagctgcacc gtcacggccg
     3001 gggcgatcgt ggcgccggag gcggcgtccg tcggctgctg gacgaacgcg acctgcgccg
     3061 cgaccggaag ctcccactgg acgctcgcgg ccgcgggggc ggctgcgttg tcgaggaggg
     3121 ccgcgtcctg agcggccccg gcgggaacgc tcgaggtcac cgtgcccgta accgacatcc
     3181 ccgtgacggt gaccgtgtag atcgcgggtc cgccggtgat gaccgccgac gtcggcaggg
     3241 ccgagccgcc gaggacgagg tccgtggccg ggtcgtcgaa acccgtcacg ttctcggaaa
     3301 acgtgacggt gaactcgatc tgggtgccgg acgtgggatt cggctggccc gcggcgggag
     3361 tcacggcgac ggtcgggggc gcgttgtcga agttgatcac cgcgtcggcc gaggccgtgt
     3421 tcgggttgag ggcctggtcg aggaaggcgc cggccagcag cttcgcggcg accgtcccgg
     3481 ttccgttcat cccggagacg gcgacccgga actgcttggg cttcgtgatg tcgggccagc
     3541 tgaccgccgc cgtcgtggga ttcgccgtcc cggagaggcc gatctcgctg tctgcgatgt
     3601 cgagcggcgc cgtggagacc gtgtcgctca cgtcgagggt gaagtagacg gtggccgcgc
     3661 tcgtgggatc gggctggccg gcctcggcga ccatcgtggc cgtcggagcc gtctggtccc
     3721 actcgatctc gttgtcgctg tagacggggg cctccgagtc gtagccggcg tcgcacgagc
     3781 cgctgaagtg cccggcggcg caggcgccgc ccgcggccat cgtgagcgtg atcgtgccgt
     3841 cccccgtggc ggtgaccggg accgtgtaga cgaggcccgg cgtgaccggg acgagggcgc
     3901 cgaaggctgc tccgctcgca ctgctcgtcg cgaggctcag gtcgctcgtg tcgaagctcg
     3961 cggggtcgat cggctcgccg aacgtgatcc tgaagcccga cggcttgttg ggtccggccg
     4021 ggtcgcccgc gagggacggg atgatctggt cgatcgtcac gtcggggtgg gtgtcgacga
     4081 cgttgaacgt cacggagatc gacgaggcga gggcgttcgg gatctcgtac tcaccgaccg
     4141 gtgggtccgg cgggacccac tcttggggca tgccgttgtt gccgaggtcg gcgaaggcga
     4201 agttcagcgt gcagctcgtc tcggcggccg gggctgcgta ggtcagctgc gacagcgcgt
     4261 agcggacgtc cgcgatgctc cccgcggtcc ccgcgaagac agtcgtgagg ttgttgccgc
     4321 tcggctgccc ggagaggttg acggcccccg ccaaaccgag ggtcgcgagg atcgcgtccg
     4381 ccgcgggctg ctcgaggtcg aggcctgtgg ggctcgtcag aatggcgttg accgagggga
     4441 acgcggcccc gccgaggttc gcgaagccgg agacgctccg caaggcgaac tggccgcagc
     4501 ccgcctgcgg gagccacgcg acgaggagca tggcgtcgtg gggttcggga atgagcgggg
     4561 cgggggcggc gtcgtaggca ccgtcgcacc ggttgttcgg gctcctgagg cagagctcgg
     4621 ggtcgacgac cgagaagagc gccgggtccg aggtgctggg agggtacgcg ttctccacgc
     4681 cggcggccgc cgtgagcgcc gaagccggtc ccgcgagcgt cggcccgccg ttgggcccct
     4741 cgacgcgcag gatgatctcc gccgtggcgt tcgtcgagct gacggaaccg tcgttgacga
     4801 ggatgtcgat gttcggcgtg ccggcgcccg gcggggtcac ctcgtcgtac gtcccgtcct
     4861 cgtagtccgc cgccgggatg tactcgaggt cggcgagcgc tccgttcagc tggtcgagcg
     4921 tgccgttcat gtagacggtc aggaccgggc caccccagat ggcggggtcg gtcgggttct
     4981 cgacgatcgc tcccgaggcg gtccggtaga cgacgggaga ggactggacc tgcgtggtgc
     5041 ccgccatgaa gagagtgccg ccggtgccgt ccgtgaggct gatctggacc cggccgcaga
     5101 gcgagatcga ccagccgttc cccgccgtcg ggttgcagga gccggagatc gacgcgtcca
     5161 tcgtgagcac gcggctctcg ttcgagatcg agtcggtgcc tgtgaactcg atcgtctggc
     5221 cgacgccggt gtagacgatt cggggcgcgg tgatcgtgga gtcgcctgcc tcgacgggag
     5281 tcgccacggc gatcgcggcg ccgacgacgg acagggcgac cgccaggcgg cggcgcagtc
     5341 gcgggcccat ggtcgtgcag aatcccggag cgacggtcga tcggctcacg tgcgtcatgg
     5401 gtcgcgtgcc tcccgcacaa gaaggtctcg gcctcccgtt cgtcccggca ggtcgattcg
     5461 gactttatgg ttttgagatc cagtctatta gaggaggggg cggagtcaag gacgcagatt
     5521 gtgcgaagtg tcacgtcctc ggcgcgtgcc ggcgtttctt cgggtctttg ccgaccagct
     5581 acccgccgat cgaggccatc gacgagccgg tcgtcatgtc gtccggggga gccttgccgc
     5641 agacgtagag cgaggcggcc gccgcgacgt cactctcgcc gcgacgcagc aggtcgacga
     5701 gcgggaaggc gtgctcgtcc atcaggcagc ggcgaaggcg accgcgggcg tcgaggcgca
     5761 agcggtcgca gtcgccgcag aacgcgtggc tgaccgggtc gatccagccg gtctccacgg
     5821 accagccgtt ccactcgacc gccgcccggc gcgccggggc gctcgggcga tccggcaggg
     5881 gggcgatgcg cgcgcccccg gcgacgagcc actcccgcac ggcggcgccg gtgacgcgct
     5941 cggcgattgc ccagccggct tcggtgccgg tctgcatcag ctcgatgaat cggacctcga
     6001 ctcccttttc cgccgcgagg gagagcaggg cggggacgtc gtcgcggaac gagctgcgcc
     6061 ggaggaccga gttgagcttc agcggggtca gcccggcgtc gagggcggcc tcgacccctt
     6121 cgagcgcctc ctcgacgcgg cccccgcgcg tcagctcccg gaagcgttcc gggtcgagcg
     6181 tgtcgagcga gacgttcacg cgtgccagtc ccgccgcggc gagctcccgt gccgaggcgg
     6241 cgaggcgggt cccgttcgtc gtcatggaga cctcgcggac cccggggatc gcggcgacgg
     6301 ccgacacgaa ggcggggagg ccgccgcgaa cgagcggctc gccccccgtg accttcaccc
     6361 ggtcgacggc gaaccgctcc gcgagccacc gcaccgcctc cgccagctcg gccaggcgcg
     6421 ggatctcgcc ccgcgggacg agcggcacgc cgacctccgc catgcagtag gcgcagcgga
     6481 ggttgcagcg gtcggtgacg gagacgcgaa gcgtcgcggc tcccgtggct cgcgggtggt
     6541 ccacgcggac ctcgtcaacc ggagccggag ggggaaggtg tggacgaggg catcggggat
     6601 ctcctttccg gaacggaggc cgccccgttc cgggaggcga cgcccgaggc atcgagaccg
     6661 gcgccgggtc cggcccgccg tccctccggt ggggacctcc cttcaggggc cgggaatcgg
     6721 ggacggcgag atattagcgc gggcgacctc ctcatcgcgc cggacccccc ggacgagggc
     6781 tagactgcat ggaacgcccg cggcaaacga tgcgatccgg tggaggggga gcggtgagcg
     6841 atcggtccgc acccgaatcg gaaaagagac gcttgagcgt gctgccgtcg ccccggagtc
     6901 cggccgcgga cagggggacg gacgatgcct gaaggcgcgt tcctcgcgct cgtccacaac
     6961 gcgagcctcc tcctcgcaat ggcgctcgtg tacgacctcg cgacgacgcg ctggtggggc
     7021 gacgagagcc ggcttcggca ggtctccgtc ggagtggcga tcggcggaat cggcatcgcg
     7081 ctcatgctct cgtcctgggc gctcgagccg caggtcatct tcgacacccg ctcgatcctc
     7141 ctcggggtct cgggtctctt cttcggcacg atcccgacgg tcatcgcgat ggcgatgacc
     7201 gccgcgctcc ggatctggca gggcggcgtc gcggctcgca tgggggtcgc cgtcatcctg
     7261 acggctggag gtatcggcct cctctggcgc tatcggcgac gagcggcgct cgccgacctc
     7321 ggagcgaagg agctctacgc gctcggtgtc gccgtgcacg tcgtgatgct cgcaatgacg
     7381 gcgacgctgc ctcgggagct ggggtttcgg atcctcgccg ggatcggcct tcccgtcctc
     7441 gtcatctacc cggccgccac ggcggcgctg ggcctcctca tggtcaaccg gctgcgaagg
     7501 aagcgggatg ccgaccaggt tcaggagagc gaggagcgca cgcgggcggc cctctacggg
     7561 gtcggggacg ccctcatcgc cacggacgag cggacgtgcg tcatgcggct caacggggcg
     7621 gccgaagcgc tgacgggatg gcgcgagccg gacgcggtcg gcaggccggt ccgcgaggtg
     7681 ctccgcctcc tttccgaggg cccgggagcc gaggaggtcg acccggttac cgacgtcatg
     7741 cgcaggggcg agctccggat gatcggggag gggacagtcc tcgtgtcgcg ggagagcgtg
     7801 acacggccgg tgctcgggag cgtcgcgccg atccacggtc ccgacggttc cgtggccggc
     7861 gcggtcgtca tcgttcgcga ccagacggcc gagcgggctc ggaaggctgc ggcacgcgag
     7921 cgcgagagcg tcttccaggc gctcttcgac cagcggtcga tcggcgtcgc tcacgtcgag
     7981 acgtcgaccg gccggttcct ccgggtgaac gagcgcttcg ccgagatcgt cggctacccc
     8041 cgcgaggagc tcggcgacct ctcgttcgac agtctcaccg agccgggcga cttcgtccgt
     8101 gaccgcgaga ccgtcctcgc gatcctcgaa ggccggcggc cgaacgagtc gatcgacgtc
     8161 cgctacgtca ggaaggacgg ctcgtacgtc tgggtcacgg cctccatctc gccgatatgg
     8221 gccccgggcg aggagccgtc gacgcacatc ctcatggccc tcgacgtcac ggagcgtaaa
     8281 ggggccgaag cgcggatggc cgacctgaac gccgagctgg agcgccgcgt ggcccggcgg
     8341 accgagctcc tgacggaggc gaaccgggaa ctcgaggcct tctcctactc ggtctctcac
     8401 gacctgcgtg cgccgctccg ggccatcgac ggcttctccc ggatcctctc cgaagagaag
     8461 gggcgcctgc tcgacgacga ggggcgccgt ctcctcgggg tcgtgagcgg gaacgcccgg
     8521 aagatgggcc agctcatcga cgacctcctc gcgttctcga ggtcctcgcg ggcggagatg
     8581 acgaagacgc gggtggatat ggccgagctc gcgcggacgg cgttcgcgga ggcgcggcag
     8641 gcgagcccct ccgccgaccg ggtcgagctt cggatgggag agctgccgcc cgccgtgggc
     8701 gacgccgcgc tcctccggca ggtctgggcg aacctcgtcg gcaacgcggt gaagttttcc
     8761 tcgggggtcg agcgaccgct cgtcgagatc gagggggagg tcgaggggcg ccgggtcgtc
     8821 taccgcgtcc gcgacaacgg agccggattc gacatggcct acgcccacaa gctcttcggg
     8881 gtcttccagc gcctccacgc gacgtcggag ttcgccggga cgggcgtcgg gctggcgctc
     8941 gtcaagcgga tcgtcgcccg tcacggcggc gacgtcgcgg cggagggggc ggtggggagc
     9001 ggggcgaccc tctcgttctg gctcccgctc gcggggccct cctcgggcaa cgacctggtg
     9061 cgcgacaccg tcgcgccata gcggggccgc aggcgcgggc ctcggtggta ccttgccgga
     9121 gatgaaggga acgaacgacg tcgcggagcg tcgcgagaga ccgatggcgc tcctcggacg
     9181 gtacctgcgc ccccaccggg cgctcgtggc gctcacgctc cttctcgcgg gtgtcgccca
     9241 ggtcctgacg ctcgtcgacc cgctcatctt cggccggatc gtcgacgact acgtcctcgc
     9301 gccgggcggc cgccccgagg ccgagctcgt ccgcggcgcg ctcttctggc tcctcgtcgc
     9361 ggcggccgtc gccctcgccg cccgtctcgc atcggccctc caggaatacg tgatgcggct
     9421 cctcgtccag agcgtcggcc gggcgatgtt cgacgacggc ctccgccgga cgctccgtct
     9481 cgcgttccag gagatggaag agcgcagcag cggcgagacg atggcgctcc tgacgaaagt
     9541 gcggaccgac accgagcgat tcgtgaacgc atccgtcacc gtgctctatt cctcgctcgt
     9601 cggggtcggc ttcctcgtct ggtacggcgt cacgcgctcc tggctcctga tcccggcgtt
     9661 cgggatcggc gtcctcgtcc tcggctcgct gaccggtctc ctgagccggc ggatcaagac
     9721 cgtccagcgg caggtcgtcc gggagacgac gcgtatgtcg ggggcgatca ccgagtcgct
     9781 ccggaacatc gagctcgtca agagcctcgg gctgacgcgg gccgagttcc gccgcctctc
     9841 gggctatacg cagcggatct tcgacctcga gatggcgaag gtgaggcgcg tgcgaggact
     9901 cgcgttcctg caggcggcga tcatcaacgt cctccggcag tcgatcctct tcatcctcct
     9961 ctggctcatc ttccgcaagg cgctctcgcc cggagagctg atcgccttcc agttcatcct
    10021 gaacacgatc ttcgggccgc tccagcagat ggggaacgtc ctcctgaacc accgggaggc
    10081 cgaggcctcg ctcgccgcgt tcgggaagct gatggcgacg ccggtcgagg agcggccgga
    10141 ggagccggtc gacgtcggcg agatcgagag cctccgattc gagaacgtca ccttccgtta
    10201 tcgcggggcg gcggaggacg cgctcgacgg catcggcttc gaggcgaagc tgggcgacac
    10261 ggtcgcgttc gtcggtccat cgggctcggg caagtcgacg ctcgtcaagc ttctcgtggg
    10321 gctgtaccgg ccgacgtcgg gcgtcatcac gatcgacggc gttccgagca acgagctgcg
    10381 ctacaaccgg gtgcggcggc agatcggctt cgtgacccag gacccgcagc tcttctcggg
    10441 gacgatccgc gagaacctgg cgctcgtgaa ggaggacgcg acggacgagg agatgctcga
    10501 ggcgcttcgc cgcgccgcgg cgacgcttct cctcgaacgc tcgggtctgg ggctcgacac
    10561 gcgcctcggc gaatcgggtc tccgggtctc gggcggcgag cgccagcgcc tctcgatcgc
    10621 ccgggcgctc ctgcgccgcc cgcggctcct catcttcgac gaggcgacct cggccctcga
    10681 ctcgatcacc gagcaggaga tcgccgcgtc ggtccgcgac gtctcgggga gccggcagca
    10741 catcgtcatc ctgatcgcgc accggctcag cacgatcgcc cacgccgaga cgatccacgt
    10801 cctcgagcgc gggcggatcg tggagaccgg gagccacgac gagctcgtcg agaagagggg
    10861 cctctactac gcgatgtggc gccagcagat cggcgagcgc ggcccctggg gcgcgccgcg
    10921 gcgcgaggcc ccggccccgg aagaggcggg cgtcgcggac gacgtcctcg tgccggaagc
    10981 ctgaagacgc gcgccggtct cgcccggcgc aaccgatggc ccagcggcga atcacgtctc
    11041 gctagggggc cccgcggtcc gatgcaggta tcctctttcg atgtcccgac gtctcgccac
    11101 gcttcttctc tccgtcgtcc ctctgctccc ggccctcgcc cgctccgagg acgcgcctcg
    11161 cgccgctgag ccctggctcg acacgccgct cgaagcgccg gttgccgatg tccgggcggc
    11221 tgccgagaag ggctggccgc acggctccgc ctccacacgg ctccagacgc tcctggtgga
    11281 ggaccgccac gccttcgatg gcgagggaaa gcggaccctg acgcggcggc gcgtccataa
    11341 ggtcctgacg gacgcgggcg tcaaggacgc cgcgaccgtg gaagcctact ggaagccctg
    11401 gcgcgacgag ccgcccctcc tgcgggcgcg cgtgatccgc aaggacggca cggcgctccg
    11461 gctcgacccg gcgaccgcga acgacaagcc ggtctccgag gacgaggacg tcttcgacga
    11521 tcgaaagctc cggcaggctc cgcttccggg cgtggagagc ggcgcggtgg tggagaccga
    11581 ggtggtgacc gccgagaaga ctccgctgcc tggcgggacg cgctacttct ccccgctcgg
    11641 atggagagag cccgcccgcc gctatcgcat cgtcgtggag tcccccgacg cgctgccgat
    11701 caccttcaag gtgcgcggta tccgcgacct ggcgccgcac gtcgagagga aggacggcgt
    11761 caccgtccgt acctgggacg tcgtggagcc ggagccgttc gtcgagatgg acgacggccc
    11821 tctcccgtcc gacgtcgaga cgaacgcgcg gctggagatc gccacggggc gctcctgggc
    11881 cgacgtggcg gcgacgtatt cccgcatcgt cgacggtcgg atcgcgaagg ggccgaaggt
    11941 tccgctgccg gcgggcctgc cggcggaccc gcgagcgaag gtccaggcgg tgctcgacgc
    12001 cctgcggcgc gacgtccgct acacgagcct cgcgttcggc gaggcggaca tcgtccccgc
    12061 cgcgccctcg gacagcctgg cgcggcacta cggcgactgc aaggaccagg cgaccctcct
    12121 ggtcagccag ctccggcagc tcggcttccc ggcccacgtg gcgctcctcg acagcggccc
    12181 cggacgggag atctccgcgg agctccccgc gctcggcggc ttcgaccacg cgatcgtcca
    12241 tgtcgccgac cccgagctct ggatcgaccc gacgtcgctc tacagccggg ccggcgacct
    12301 tcccctcggc gatcgcgacc gctgggcgct cgtctgtcgg gaaggcgtcg agtcgctctc
    12361 ccggacgccg cgcctgcgcg cagccgacag catcgagcgc gtcgagctga ccgtgatgct
    12421 cgccgacgag gggcgctgtg atgtgcgcga gctctcgacc tatgccggct gggtcgagga
    12481 gcgcctgcgt gcttcctggg acgggaccga ggagaagcgc tttcgcgagc acatggagaa
    12541 gtacgcgacg aacgcgctcg gcaaggcgac gttgacccgg gcggagaggg gggaggcccg
    12601 cgacctggcg aagcgattcc agctgctgct cgaggcgaag ggatccgctt tcgcgacgac
    12661 gggagaaggc tctgcggcgg ttcaggtctc tcgcggcttc gggctcggca gcctgcccaa
    12721 gaccacggag aagaagcgcc gaatggacct cgcggtgacg ccgttcacgc gcgagatcgt
    12781 cgtgcgggtc gtgccgccgc ggggcttcga ggtcgtcgaa acgccgccga gccaggaaga
    12841 ggttctcggg ccggcgaccc tcgcgatcgg cagctccgtc gacggggagg gcgtcgcttc
    12901 cgtgacgctg cgcctcgtcc tcgaccggaa ccgcctcaca cccgacgagg tggccgagct
    12961 ccactccgcc gtggagaaag tctcggcagg agggcttctc tcggtgcgcc tccttcacgc
    13021 ctcggagatc gacctcgccg aagggcgcat cgccgaagcc ttcgcgaagc tgcgacagga
    13081 gctgcgggat tcgcgagaga gcggcgtcgc gcatcgccgg ctctcgagcg cgctcctccg
    13141 ggtcggcctc ggagacgccg cccgccgcga ggcgcgcgag gcggtccgga agtcgcctgc
    13201 ttcccctgcg gcccagcgtg cgctggcgcg ggcgctcggc cacgattcct tcggtcgcgc
    13261 ctatcgcccg ggctgggacc cggacggcgt ggccagggcg ctcgccgagg tcggcaagct
    13321 cgatcccgaa gacctcaccg tcctggcgga cgaggcggag gccctcgagt tcgacgccgc
    13381 cgggcatcgg tacgcggatg ccgggagact ccgagccgcg atcggagtct acgagaagct
    13441 cctcgcgaag gccccccgcg ccacggcgct cgttcgcgcc ctgtcgacgg ctcttctctt
    13501 cgccggccga gcccgtgagg tgacctccct cgtgaacgga cggtccgact ccgctgcgct
    13561 ttccgacgtg gcgatcgccg ccctcgccgt gagcgacggc gcgcagcggg cgcgggaaga
    13621 ggggcgccgg cagctcgccg cctccgaacg gagcgccggc ttcgagaagt cggccgagct
    13681 tctcgtgagc ctgcgccgat atcccgaggc ggccgagctt taccgagaag cggcccgttc
    13741 ctcggagaag gccgccgcgc tcggcgctcg cgccgacttc tcggcgcgca tttccagcac
    13801 gcccccggcc ctggacggga aggacgcgga gtccgttctg cgggcgttcc tctaccacgc
    13861 ctacgctcct cggaagccgg ctcccgaaga gctacacccg ttcgccgccc gggccctgcg
    13921 cccgtttatc cctgagttcg tcgacgcctt gcgcagcgtc tccctgtcgt ccgggttcgc
    13981 gtcgcgggag ctggcgcggg acgtctcgac cttcggcccc gagtggttct ctcaggggag
    14041 cccggagtcg ggagtccgga tgcgcgccat cgggaacggc cccgaggcgc gcgataactg
    14101 gatcgcggaa gaggagggac gtctgcgcgt cctctcgatc gggatacccg atcccctctg
    14161 ccgtcacgcc gagacgctcc tcgggaaggg agacgtggcc ggcgcacgca cctggctcca
    14221 gcgggcacgc gagatggacc tcgaggagag ccgtgagggg ccgccgctcg atcgcccggt
    14281 gacacgctgg ttcgacccgg cgagcgagga cccctccgag ctgctccttt ccgcgatcgc
    14341 ctgccagcag gtggagcttc tcggcgacga cgccctcgag aagctcgacc ggctcgccgc
    14401 cgagcgaccc gaggacgacc gtcggaaggc gctcctcgtc aaggccgagt tcgcgatggg
    14461 agacgccgct cgcgccctgg agatcgccag ggacctctac cggaggaacc ccgacagcga
    14521 gactgcggcg accgccctgc tcctcgccct gcaggagaac gggcactcgg ccgaagccat
    14581 ccggacggca caggcgcagc tcgagaagtg gccccggcag ctcgacgtga ggcgggtgct
    14641 ctcggagttg cgggctcgcg caggcgactt cgaaggggct gcccgcgaag gcgaggagct
    14701 cctcgggctg ccgcgctcga acgcgctcga tctcaacaac gccgcctggc acaggatggt
    14761 cgcaggccag ctcgacgaga agacgctttc cctcgcgcaa cgtgccgtgg aaggtacggg
    14821 tcggctcgag gcgacgaccc tgaacaccct gggcaccgtc tatgccgaga tgggccggcc
    14881 ttcggaggcg agggccgtcg tgctggaagc catggacctc gccgagtcgg cggagcccga
    14941 cccctccgac tggtacatcg tcggccgcct gctcgagagc ttcgggctga tcagcgatgc
    15001 gcgggaggcg tacgggaagg cgctggtcgt cccgtcgtcg gacgcttcga agccccgtcg
    15061 ccgcgccgag cgcgaagccc gagaccttcc gggcagtccc tcctggctgg ccacccggcg
    15121 actcgctgcc ctgggagagg cgccagccgg ccgctgattc cggacctcgc gacagagtgc
    15181 gacggcgagc ggtcgccgag cccggaggct tccaaaccga ggcctcgtcg ggttcgactc
    15241 gggtgtcacc ccgggtcaga agttgatcgc gcccaggggt gatgacccgt tcaaatcccc
    15301 gaggtggatc aatttggtcc ccacctggtg ctgcgaaagg cctacttacc gctcccttct
    15361 cccggcacga ctcttgagtg ggggcggccg tgacgcccgt cacgaattca gagtcggagt
    15421 ccgaattgag gagaggttgc atgcttcatt ggcggtggac cacgcccatt gccctgcttt
    15481 tctcagggtt cctgatgatc ggctccgctc cggcgcaggc gcagccgagc accgaccggg
    15541 tgccgacgct cgccgagcag ctgaagaagg ccggggctcg ccctggtacc gccttcgagc
    15601 ggctcatcaa ggagaaccag gacttctcgg tcctccgagc tgacgagaaa gcagacaccg
    15661 gaagggtccc gccctggctg aaggtcatct ggcggaaggc gaatccggac ggtcactaca
    15721 cggccgccga cccgacgggg ggctacccct tcgtcctcaa ggaggtcctc gagtggatgc
    15781 agtcccacca ggacctccgc ccgggcctcc ccgacgcgaa cttcgaggcg gggtacgaca
    15841 acccgccgga cggcgtcggg acgtgggacg aggacctctc ggtcacggcc ctcgccacct
    15901 ccatcgggac gaactaccgg atctcgggct cccagtcgtc gccccggagc gagtccgaca
    15961 tccggatcaa ctactggaac ccgaaccaga tcatcggcgc ctcgaacaac atcagcggca
    16021 gcggcttcca ggcccagttc tactcgctgg acggcgggac gacctggggc cagacgacgc
    16081 tccccggcta cagcggcgac gcgttcaact ccgacccgac ggtcgactgg agctccgacg
    16141 gcaccgcctg gtcctcgacc atggggatca acagcaccgg gacgatcctc aagatccgga
    16201 tgtacaagtc gacggaccag gggcggacct ggacctacga cgcgacgatc tcgggcagcc
    16261 agaccaacac ggacaagcag atggtctgga tcgaccacag cgccacctcc ccctacaagg
    16321 acaacatcta cgccatctgg cacaacggaa acccggtcta catgaaccgg cggaactcca
    16381 ccggctggct ctcgacgccg atccaggtga gcgggagcca gacgacgggg accggcatcg
    16441 gctccgacgt gaagacgaac tcctacggtg acgtcttcgg cctctggccc gacaccggct
    16501 cgcgcggcct ctacgtcgtc aagtcgacga acggcgggac gagctacggg accccggtca
    16561 agctcgcgac gacctacgac tcgtacgaca tcggcgtccc ggccttcaac ggccgccgca
    16621 tcctcatcta cgtcaccgcc ggcgcctacc ggacggcgag caagaacctg gtctacgcgc
    16681 tctggaccga cctctcgggt gagaccggct gcacgaccgc ctccaacgag ccgggctcct
    16741 cggtcacctc ggcctgcaag acccggatct ggttcgcccg ctccacgaac ggcggcacct
    16801 cgtgggggac gccggtcaag atcaacaacc agtcgggcaa gaacgaccag ttcaacccgt
    16861 ggctcgtggt cgacgagacg aacggggcga tcgcggtcat gtactacgac acggtcgacg
    16921 acgcgaaccg gaagaagacc cacgtctact accagtcgtc cttcgacgac ggggccacgt
    16981 ggggcgcgcc ggtcaaggtc acgaccgctc agaccgacga gaccggctcc ggcgccgatt
    17041 ccggcaacca gtacggcgac tacaacggcc tctccgggta cgccggcgtc ttcttcccct
    17101 cctggaccga ccgccgcaac gccgcaagcg aggagatctg gaccgcgaag gtcaccgact
    17161 cgggcagcgg cgcgacctac agcctctccg gcacgatcac gctgagcggc tccggcctct
    17221 cgggcgtcac cgtcaccgcg ggctccggct cggccacgac gagcacgagc ggcgccttca
    17281 cgatctccgg cctcgcgccg ggcagctaca ccgtcacgcc gagccggtcc ggctacacgt
    17341 tcagcccggc cagccagtcc gcgacgatca cgagctcgaa cgtcaccggg gtcaacttca
    17401 cggcgacggc ggtgtcgacg acgtacagca tctcggggaa cgtcggcacg gcgggggcca
    17461 cggtcacggc gggctccgcg tcggcgacga gcgacgcctc gagcaactac tctctcgcga
    17521 ccctcgtggc cggcacctac accgtgaccc cgacgaagac cggctgcacg ttcagcccgg
    17581 ccagccagtc ggtcacggtc acctcggcga acgtcaccgg ggtcaacttc accgcgagct
    17641 gcccgagcgg agacctccag ctcacgagcg gagtgccggt gaccggcgag agcgtggcca
    17701 agagcgcctg gaagtactac tccatcgtcg tcccctcggg cgccacgaac ctgactttcg
    17761 ccacgacgag cgccacggcc gacgtcgaca tctacacgca gaacggcgcc aagccgacgt
    17821 cctcctccta catctgccgg ccgtacagct cctcgggcaa cgagacctgc tcggccacga
    17881 accccgccgc cgccacctgg tggctcggcg tctacggcta tgccgccggg agctacaccg
    17941 tcaccgggac ctacaccctc ggcgggacga cctacaccgt ctccggcagc gccggcacgg
    18001 caagcgcgac cgtcaccgcg gggtcggcct ccgcgacgag cgacgcctcg ggcaacttca
    18061 cgctcacggg cctcgccgcc gggacctaca cggtgacccc gacgaagagc ggctgcacgt
    18121 tcagcccgac ctcccgctcc gtgaccgtct cctccgcgaa cgtcacgggg gtgaacttca
    18181 cggcgagctg cgggggcggc gagacgacgc tcttcaacga cggcttcgag agcaccggct
    18241 ggtcgcaggt tgacacctcc ggcaccgcgg gctcctggac cttcgccacc tcctcgacct
    18301 atcccacgac gtcgcctcac ggcgggacga agctcgcgaa gttcaactcc tacaacgcct
    18361 cgagcgggag ccagacgcgc gtctaccggt cctccggctt cgccgtggcg agctcctaca
    18421 cgagcgtcac gctcaagttc tggatgtacc acgacaccgg atactcgtcc aggaacgacc
    18481 ggatccagcc ccaggtctcg gtcaacggga cgacctggac gaacgtcggc tcggcgatct
    18541 cccgatacag cggcagcacc ggctgggcgc aggtcacgat cgacctctcg gcctacaaag
    18601 gccagacggt ccgcctcggg ttcctcggca tcagcgccta cggcaacaac atgaccctcg
    18661 acgacgtgac ggtgacggcc cagtagggcc ttctccgcgc gcccgttctt tcccgacgcg
    18721 gggcgccttc gggcgccccg cgttccgtat cggcgcaggc ttcgcggcac gtcgtcccgc
    18781 gatcagaacc tccaggagac cttggcctgg acgacgtcct gggacgggac gtggtgcagc
    18841 gaggcgaggt cgtcggaaag cccgagcccc gcgccgccgc gctcgttggc cgcggccccc
    18901 ttcgtgtaga cgaggtacgc ggtcgagccg cggcggaact gccattggcc gatcaggttc
    18961 agggcccacg tccggtcgga gaacgtcgtc ggcccggccg ccgcggcggc gacgcggctt
    19021 ccggcatcgt tccaggcccg ggtctcgcgg aagctccacc gcgcggcgcg ccactggctg
    19081 aagagttgga ccgtcgcgcg cgtcgacacg gcgaaggaga ttcggatcga ctggtccagc
    19141 tgtctcagcg cccggaggcc gacgaccgga gccccgggcg tcggcgtctc cagccagcgc
    19201 tcctctcctg aggcccgctg gtacgaggtg tccagctcca cctcgagccg ggacgtgggc
    19261 cgaacgatct gcgagacggc gagctcgttc gtcggccctc cctcccacga gtcccaacgg
    19321 gcttcgcccg acaccgacca ggcggcctgc ggagcagttc cgaagctgag gatggcgaac
    19381 gggcggtcgg gcctccggag gatgagcttg cgctcctgcc cgaacgtccg gagctcttgg
    19441 tcgtcgtcgg cggacaggta ggcccccgcg atggccgaga cccgccagtc gttcgtgaac
    19501 tgcgtgccga gcgtcgcctc cacctcgcgc gtcagcgtgt gcccggcgtg gtcccggcgg
    19561 accatccctc cgaacgcgat ctcccagttg cggaagactc cggcggtgca gtcccactgc
    19621 cggcggatgt cgacccatcc ctgccgaacg tcggggcggg cgagatagcc gaggtcgttc
    19681 gggtcgaact gcggcgagag ctccgtcagg tgaacggtcg ccgtccaggg agtccggaac
    19741 gtgtggacca gctcgaggtc ggccccccag ccgcgtcggt cgttttcgcc cgagccggca
    19801 atcgaccccg ctaggatccc ggtcagcttc gtccgccgcg acgccgagcg gtacgagccg
    19861 tcgacggccg ccacctgcgc ctcccgcccg ttcgccccgc gctgacccgt ccagccgacg
    19921 aaggcgccga cgtacccgcc ccggtcgccg agcgcccggg agactcgagc caccccgtag
    19981 gcctgcgcct cctcgagcgt cgcggtctcg atccggccgt cccggtcgcg gagctccgcc
    20041 tcggcgcgct gcgtcacggc cccgagcgcg gccatccgcc acccgtcgtc cgagtgccgg
    20101 ctgtagcgcg ccgcgccgag gaggtcgacg ttcagcggcc gctcgaggac ggtcgtcccc
    20161 tccggtgcgg ccggcggcgc gagtgcgtgg ccgatgcgcc ggctgtagaa gagctgcagg
    20221 ccggggaggg tgaaggcgtc ggcaccctcc gtgaagaacg gcctcttctc ggggaagaac
    20281 gtctcggtcg tcgagaggtt caggacctgc cggtccacct ccacgtgccc gaagtccggc
    20341 aggagcgaga cgtcgagctg ccccgtcgct ccgatgccga gggaggcgtc ggcgccggcg
    20401 cggaacgcct cgccgaggtc gtcgtacggc tcggcggtcc tcaccttcga ccgtgccgag
    20461 gcgtgggccg aaaggtcccg ccgccgcggc ggcttcgcga cgacgacctc ctccagcggg
    20521 tagaagtggc tcacgaagcc ctgggcgcct cgcggcacga ccatccaccc cgtcacctcg
    20581 tacggggact ggttctcgcg gtagaagtag atccgccacc tctgaggcgc cggcgacgcc
    20641 ttcgggcgga ggacctcgag cggaatccgg atctcgacgc tccagcccga cgcgcccatt
    20701 gacgtcgccg agtcccagac ggcgtcccag gtgtcgtcat cggccgcttc ctcgaactgg
    20761 aggaggtccc gctgcgcgtt cgcggcgttc acccggaaca tgcgcgcgtt ccggccgcgg
    20821 ccctcgacgt cgatcgagac gccgaaccag tccgagtagc tgtcgcggtc ccgccggtgg
    20881 acccgcgcgg cgatggcggc gggtcctccc gggttctcga gcgaggctcc cacgtagagg
    20941 tacgcgtcgt cgaagaggac ccggacacgc gtctcgagcc gggccggctc gccgcgattc
    21001 gggctccact gccggaagtc ggaggccgcg gcggcttgcg tccagacggc ctcgtcgaga
    21061 cgcccgtcca cggtcaaagg tcccgcgagg cgagccgccg cgagcgaggg cgtcggcgcg
    21121 gccgagagcg gagaggctgc ggcgaggagc gccgccagga cccggacgtt catgtgtcac
    21181 cctcccggat gaacgatcct gtcgacccga cccgcgaggc gcgcgtgccg gaagtcgcgc
    21241 ttcgcgaccc atgacttccg gcactccccg cggctcctcg cgggcctatc ctcgggccat
    21301 gacgctccgg cccgagctgg accgatggat gcggcgtctc acgctcctca tgtggggcgc
    21361 ggtcggctgg gcgatgctgg ccgagaacct tcacagggaa gggacggtgc ccgtcgcctg
    21421 cctcctctcg tgggcggccg cttaccttct ctgtggcctc tccttcctcc tcgcgagcag
    21481 cgaggggatc caccgctggc cccgctggac ctgggtcgtc atcctcctcg cgcagaccgg
    21541 ttccgtcctc ttcatgacgg gctggtggag gagcctcgcc ggagggttcc tcctcgtctt
    21601 ctccgcctgg caggccgccc gtttcctttc cttcggcgcg tcgctcgcgt gggtcgtctc
    21661 ccagtccgtc ctcttcggcc tgatcctcgt ctcctcggac ccgtcggctc cgtggctcgt
    21721 cgtcgccgtc ttctgcggcg gcctccagct cttcgccttc ctggtggccc acctcgtgcg
    21781 gcaggaggag gcggcgcgac tcggcctcgc ccgatccaac tcggagcttc gggatgccca
    21841 cgagctcctc gcccgggtga gccgcgggag cgagcggctc cgcgtcgctc gggagctgca
    21901 cgacggcctg ggacagaagc tcgtcgccct caacctggcg ctcgagtccg ccgagaggga
    21961 cctctcccgg ccggtcgacg ggctcgcccg cgcgcggaag ctcgtccacg gcgccctcgc
    22021 cgagctccgg gtcgcggtcg gacggctccg cgaggagccc ggtccggacc tcgagtccgg
    22081 tctgcgcgag ctcgtggccg acatcgaacg cccccgcgtc catctcgagg tccgcggcac
    22141 gtgcagccgg gtcgacgcgg cccgggcccg ggccctcctg cgctgcgcgc aggagctcct
    22201 cacgaacgcg atcaagcact cgggggccga gaacgcctgg ctcctcctcg agtgcacggc
    22261 ggacggcgaa cggctcgtcg ttcgcgacga cgggaggagc gacggcgtcg tgctcgaggg
    22321 gtgcggcctg aaggggatcc gggagcggat cggcgcgctc ggcgggacgc tcgcgctcgg
    22381 ggcgcgaccg ccgggcctcg tcgcgtccgt cacgatcccg gcgttcggcg aggggccgtg
    22441 atccgggtcc tcgtcgtcga cgaccagacg ctcgtgcgcg aggggctcgt cgcgctcctc
    22501 ggctcgctgg ccgatctgga agtctgcggc gaggccgccg acggcgagga ggcgctccgc
    22561 atcctcgagg agtcggctcc cgacgtcgtc ctcctcgacc ttcggatgcc ccgcctcgac
    22621 ggcctcggcg tcctcgaagc cctctcggcg cgccccgcgg cgccgcccgt cctcgtcctg
    22681 acgacgttcg acgacgacgc ggccctcctc gaggcggtcc gtctcggcgc ccgcggctat
    22741 ctcctgaagg acgtccgtct cgaccagctc gccgacgccg tccggacggt cgccgcgggc
    22801 ggcgagctga tccaccccag cctctcgagc cggcttgtgg agaccctcgc ggggcgtccg
    22861 ggccggttcg acgccctcga cctccccgac ccgctgaccc gtcgcgagaa agccgtcctg
    22921 cgcctcgcgg ccgccggctg cgcgaacgag gagatcgccc gggcgctcgg cctcgccgtg
    22981 gggacggtga agaaccacgt ctcgacgatc ctctcgaagc tgggagtgag ggacaggacc
    23041 cgcgccgtcc tccgcgccta cgagctcggc gtcctctgac gaggccgacg tgcgcctcga
    23101 ggggccggcc tggcgcgagc acgtgcgggc aggaagggcc ggcgctctcc ggggcacagt
    23161 gccgggtgaa ccgcacccgc aggcgcgccg cctccaggcc gggtcgctcc cgggctggtc
    23221 gcagcgaccc ggcgccgcgt gggaccgcga agcgagccga tcccggaccc ggcgggcagc
    23281 ccgatcagag tcccttcacg aacgggggct tcgaggctcg cggctcggtg aggtcgaggt
    23341 ccagacgacg acctgtcgcg ccgcgatccc cctccggtcg ggcgccatcc gccggacctc
    23401 cccgaggacg cacagggcgc cctctcgccg gtggatcgac tgcgcggcgg aatcggtcca
    23461 gtcccccccg acgagcccgt ggaggtcgac gtgggaagcg gccgtgccgg accagagcgc
    23521 cgcgcgctcg acgccgccgc cgcgaacgga cccgacctgg acgccctcga gcacgtccac
    23581 cgcgcgcccc gccgtgcacc cgccggggag gagcttcgtc atcgtcgccg ccgagccgcg
    23641 ccagacgacc gggaggacgt tgcccgcgag gaaggcctcc tcggtgcgcg cgatcagccc
    23701 cacctgctct ccgtcgccca cgccgagggc ctcgctccac ccggcctcct gcgggtggag
    23761 ggaaaccggc tctcccgtac ccctccacag cacggcccgg ctgggccacc cggcggcgcc
    23821 cgcgaccagc ccgtcccgta cgccgagcgc ccgggctccc tcgccgcccg gatcggggag
    23881 gatggtcggg gcggcgcccg ttcccggcca gaggagggcg gcgcccgagg tggccgtgcg
    23941 tccgctgccg acccgggtgt cgccgtcggt agctgcagcg accgactcga ggaagccccg
    24001 cgggtggagc gatacgaccc gggcctcgcc ctcgggaccg aagcgcagga gcgcggcctg
    24061 ccctccgctc ctccggcgga cggcgccgac gaccgtcgtc tgccgagctg agcgggcctg
    24121 cagtcggagt gccccggtgt cgacgacccg gggtccttcg ccgtcccaca gacagaccct
    24181 cgaccctccc tgggcagcct cgacgttccc gacaaccgcg ccgccgcaga ggccggcgct
    24241 cccggcgccg aagaagcccc gcggaagggg caggaccgaa gctgtccagg tcatgccgcg
    24301 gaatcctacc tgccggctcc tgtcggggac gagggccggc tcccggggcg tgtagcggcg
    24361 gacaggcgag cgtcacgagg cccaggaggc gagcgtggcg gccgccgagg tcagccttcc
    24421 gggaacccca gctcccgcgc cacgagcgcc gggagagcct cgcggatctc gtcccggaca
    24481 cggcggaaca ccgggagcgc gtcgtccccc tcgccggcgt gggccgggtc ctcgaacgcg
    24541 tggtggacgg tcttcctcgc accgggaaag acggggcagg cctcgcgcgc ggaatcgcac
    24601 accgtgacga cgaggtcgaa ggggaggcct tggagcgcct cgaccgactt gctcgcgtgc
    24661 gacgagaggt cgacccccgc ctcggccatc acctggatcg cccgcgggtg gacgaacgaa
    24721 gcccgggtcc cggcggagtg cacctcgacg cgttcgccga ggaggtgtcg gacccacccc
    24781 tcgcccatct gcgagcggca ggagttgctc gtgcagagga cgaggacgcg ggccttctcc
    24841 acctcaggcc ccctgcgcgg cggcgcgcgc ggacggggcg ccgaagtacc tccgctggaa
    24901 ccagaacgcg acgttcacga ggccgatcat cacgggcacc tcgacgagcg gcccgatgac
    24961 tgccgcgaac gccgcgccgg agttgatgcc gaagacggcg acggcgacgg caatggcgag
    25021 ctcgaagttg ttgctcgccg ctgtgaacgc gagcgtcgtc gtcttgttgt agtcggcgcc
    25081 gaccttcttg cccatccaga acgagacgac gaacatcagg acgaagtaga cgacgagcgg
    25141 gatcgcgatc ctcacgacgt cgagagggat cgtgacgatg agcttcccct tgaggctgaa
    25201 catcacgacg atcgtgaaga ggagcgcgat cagcgtgatc gggctgatgg cggggacgaa
    25261 gcggcggtgg taccactcct tccccttcag cttcacgccg acgaagcgcg tgagggcgcc
    25321 cgcgaggaac gggatcccga ggtagatgaa gacgctcttc gcgatctccg ccatgctgac
    25381 gtccacgagg cgccccttca gcccgaggag cggcggcagc acggtgatga agaaccaggc
    25441 gtagacgctg tagaagagga cctggaagac gctgttgaag gcgacgagcc cggcggcgta
    25501 ctccgtgtcg cctttggcca gctcgttcca gacgatgacc atcgcgatgc agcgggcgag
    25561 gccgatcatg atgagccccg ccatgtactc gggcctgtcg cgcaggaaga cgatcgcgag
    25621 gacgaacatg aggaccggcc cgacgatcca gttctggaag agggagaggg cgacgacctt
    25681 ccggtcgcgg aagacgtcgc ccatctcctc gtacctcacc ttcgcgaagg gggggtacat
    25741 catcaggatg agcccgatgg cgatcgggat gttcgtcgtc ccgacctgga acgagttgac
    25801 gaaggactcg actcccggga tgaaggctcc ggcgaggacc cccgcggcca tcgccgcgaa
    25861 gatccagaac gtcagccacc ggtcgagaaa cgagagcttc tttgcgacgc catcgggcat
    25921 ggtctctgcc tcctgtgcgg gtccgcggtc gaggcgtgga ggggggcccc cgccctacgc
    25981 cggcttcacc gcgcggacga acgcgctccg gaacttcccg tcgacctcgg gcccgagggc
    26041 gtccacgtcg atcccggccg aggagaggaa ctcgcgggcg tcgtcgaccc ggtagatgcg
    26101 cgtcggctcg accgtgacgt ccgtgaagcc cgcggccgtc agcttcgcga catagtcgtc
    26161 ctcgtcgagc gccccggcga tgcagccgat ccagagctcg acgctcttct tcaccggcgc
    26221 cgggatctct cccttcgtga cgacgtccga gacggcgaag cggccgcccg gcttcaggac
    26281 gcggaacgcc tcgcggagga cgcggtcctt gtcggcggcg aggttgatga cgcagttcga
    26341 gatgatcacg tcgacgctgc cgtcggggag cgggatctcc tccatcgtcc cccgaaggaa
    26401 ctcgacgttc gtagctccgg cctttttctg gttctcgcgg gcgagcgcga gcatctcgtc
    26461 ggtcatgtcg aggccgtagg ccttgcccgt cggcccgacc cgcttcgccg agaggaggac
    26521 gtcgatgccg ccgccggagc cgaggtcgag gacgacctgc ccctcctcga gcttcgcgag
    26581 ggcggtcggg ttgccgcagc cgagcgaggc ctggacggcc gtcgtcggga ggagcgccgt
    26641 ctcgtggtcg gcgtagaggt tcgaggtgat cgggtcgccg gagaggccgg aggccgaggg
    26701 ggcggtgccg cagcaggccg acggcgccgg gccgcagcag ctcgtgcgcc cctccttcac
    26761 ctgcagggcg gccttcccgt acttctccct caccacgtcg cggatttcgt tgtcgttcat
    26821 ggcgtgtctc cttctatcaa gaattctcga tacgttccag aaaaaaaggg tttcaggtgc
    26881 ggcagcagct cgcggcgcac gacttcgcgg ccgcctcgag ctcctcgagg gcgacccgga
    26941 tctcctccac cgcctcgggg acgagggagt agtggatcca gcgcccctcc cgccggtcct
    27001 tcacgaggcc ggcgtccttc agggtcttca tgtggaacga gaggcgggac tgctgggcga
    27061 cgaggagccc cgtcaggttg cagacacact gctcgccgtt ccggagagcc gcgaggatcc
    27121 ggatccgggt cgggtccgac agggcgtgga agaggtcggc gagacgctcc gcgcggtcct
    27181 ccctcgcgag cgggaggccg gtccggaccg gcttgccgcg ggacgtcgtc gtcgccatgg
    27241 ggatgaatat atccacgaat cttgatatgt caaggaggag atgagattcg aagacgcttc
    27301 gtggtgcgcg gcggggcgct cgaggacgag ctcggagcgc cccgggccga agggggcgcg
    27361 gcgggctact ccggagcggc tctggccgtg agctgctcga tcagccactg ccggagcgac
    27421 agcagctcgt cggcctggcg agcgtcccgc ccgagcgcct tcaccgcggg gcgcgggtcg
    27481 tagtccttca gcatccagac gagtgtcagc ggcgagaacc cagcgtccct ctttggagcg
    27541 tcgcgcagtg ctgctccgag gtcgcccccg gcgtccagga gcgtcttgac gtcgggcagg
    27601 tcgcggacct cggagcgttc gagcagcgtg gcgagcttgc tggcgaggat ctcgcgtctg
    27661 ccgtccaccg agatcgccgc accctcgatg agggcctcct ccgggggctc gatggccgcg
    27721 aagggttcgg cgacgaggtc cacgacacag gtgtcgcgtc cgtccgacac ccggagctct
    27781 ccgaacgcag gcgccgtcgt cagagacaac gtccagcggg ggggggatcc cggcgagtga
    27841 accgagaatg cgggtctgga atcgtgtcag cctactgcca gacgcgaccc tggccttccc
    27901 acgtctcgaa gagccacgac aagaactcgc gcgtgcggcc gagacaccgg gtgagtcgcg
    27961 gccagagatc ccggatttcg ccggggccga cgaacaggaa gacgtcgtcg ggcttggcct
    28021 ggcgcatgag cttcccgatc agataggcgt gaacctcggg gtcgggatcc gcgagaccct
    28081 cgcggaactg gctgagcgtc agatcgccgt cccagagaaa gtcgggacga tcctgcgcat
    28141 cggtcagccg ttcccggggc gtgaagctca acacgcggcg agcgtcgcag acccgggaag
    28201 ggcaggaggc aatcgcgccc gcgcgaaaag gggcatcatc cggcgccacg catgagcgca
    28261 ccgggagact cccccgaacc ggcccacgtc attctcgccc tcgccgtcac accccacggc
    28321 cacctccgcc tcgaccggac tgcgggagac gcggacgatc cgacggcggg tgtggccgcg
    28381 cgcatcgaag cggccttcgc tcgcggcgtc ggcgccggcg tcctccacct cggcgcggcc
    28441 gagcccggga cccatctccc tccgtctctc gccttcttcc gcgacttcgg gcagctcttc
    28501 gtcacggccc tctcgcggct gccggacgcc gaagggcgcc gggcggcgct cgacctcccc
    28561 gtgcccgcgg acgagctcga cgcacgcgtc gccgcggcgc cgcccctcga cggcctcgaa
    28621 tacctgaccc gtggcgtcct cgagaccctc tggagcgacc tgaacgcgac ggcccggcgg
    28681 gagctcgcgg agtggaaggg ggacgtcgag tcgtggctcc aggcccggag ccctctctgg
    28741 cactccgtgg ggcgggtctg ctttcacctc gccgagaaca agacgagcga gacgaagccc
    28801 ttcgcgttcc tcgcgacgta cgcgacacgg ctctccgaag ccggacgggt ccagcaccag
    28861 ccgctcggcc acgccgtgaa gacctccggc gccgcgcgcg accgcgatgc gctgaggtca
    28921 ctcctcgtgc cggtccagcg ggccgcggag aagagcccgt tcgtaaaggc gctcgtcgac
    28981 tcgggccagg tcttccaacc gctggcgtgg acctcctccc aggcgtggtc gttccttcgg
    29041 gaggctcctc tcctcgagga gagcggcgtc cttcttcgcc ttcccgactg gtggaagaag
    29101 ggggcgccgc cgcgccccga ggtccgcgtc acggtgggcg aggcgctcgt ctcgggcgtc
    29161 ggaggcgatt cgctcctcga cttctccgtc gagctgacgc tcggcgggga agcgctcacg
    29221 gccgcagagc ggcggcagat cctcgcgtcg acggccggcc tcgccctcgt gagggggcgc
    29281 tgggtcgaga tcgacccggc cgagctgaag gaggccctcg cgcgtctcgc gaaggcggcg
    29341 gaatcgcagg gggagggcgt cggtatcggg gaggctctcc gcctcgccgc gggcctcgga
    29401 acgagcccgg gcggaggcgg agacggcggc tgggtgcgcg tcgagccggg gcgctggctc
    29461 gcgaaggcgc tcgagagcct ccggaccccg gaggggcgcg ccgctgcgga ccccggcccc
    29521 gcgcttctcg gcacgctgcg tccctaccag aaggacggcg tcgcctggct ccacaccctc
    29581 tcgaggctcc ggctcggggg ctgcctcgcc gacgatatgg ggctcggaaa gacgattcag
    29641 gtcctcgccc tcctcctcgc gctgaagaga gcgggagaga aaggccctca cctcctcgtc
    29701 gtgccggcct cgctcgtggc gaactggacg gccgagatcg cccgcttcgc cccgtctctc
    29761 tccgtcctcg tggcccaccc ctccgtcacc ccggccaaag agctggccga cgcggcgacg
    29821 gcggctggcc gcgtcgacgt cacgatcacg acctacgcga cgctcctccg tctcccggcc
    29881 ctgcaagagg tccggtgggg cctcgtcgtc ctcgacgagg cgcaggcgat caagaacccg
    29941 tccgcgaagc agacgcgcgc cgtcaaggcg ctcgcgagcc gcgggcgtct cgcgctcacg
    30001 gggacgcccg tcgagaaccg cctcggcgac ctctggtcgc tcttcgactt cctccagccc
    30061 ggcctcctcg gctcggcgaa ggagttcggg cagttcacga aggcgcgggc cacctcggcg
    30121 aaccccgatt gggggcccct ccgccggctc gtcaggccgt tcatccttcg gcggctgaag
    30181 accgaccgaa gcgtgatctc cgacctcccc gagaagaccg agatgaaggt cttctgcccg
    30241 ctctcgaaga cgcaggccgt tctctaccag caggccgtcg gcgagctggc gaagctcctt
    30301 tcggccgtcg acggcatgaa gcgtcgcggc gtcgtcctct cgtacctgat gcgcttcaag
    30361 cagctctgca accacccctc gcagtggctc cgcgacggcc gcttcgaccc gaaggagagc
    30421 ggcaagctgg gccgtctggc cgcgctcggg gaggagatcg cctcccggca ggagaaggcg
    30481 ctcgtcttca cgcagttcca ggagatgacc gggccgctcg cctcgttcct cgcgggggtc
    30541 ttcggccgtc ccggcctcgt cctgcacgga gggacgcccg tcgcgaagcg ccgcgccctc
    30601 gtcgaagagt tccagcgcga ggacgggccg ccgttcttcg tcgtctcggt caaggcggga
    30661 ggcaccggcc tcaacctcac cgaggcctcg cacgtcgtcc acttcgaccg ctggtggaac
    30721 cccgccgtcg aggaccaggc gaccgaccgc gcgttccgca tcgggcagaa gcggaacgtc
    30781 ctcgtccaca agctcgtctg ccgggggacg gtcgaggaga gaatcgacgc cctcatcgag
    30841 gcgaagaagg cgctcgccag ggacgtcctc gagggagggg cggaggcccg tctgacagag
    30901 ctgagcgatg ccgagctcct ccagctcgtc tcgctcgacc tcgccgcggc gacggcagaa
    30961 tagaagaggg tatggcttac ggatacggat acggctgggg ctacgccccc tacgtgagcg
    31021 tggcgagccg gagggcccgg gcggcgaagc aggccgcgtc gctccggaag aaggggcaga
    31081 tcctcgagcc ggtgacgatc gaggggcgca ccgtcgcccg gtcgttctgg ggcaaggcct
    31141 ggtgccgcaa cctcgaggtc tacggcgact tctcgaaccg cctcccgcgc ggccgctcct
    31201 acctcgccaa cggcctggtc ctcgacctga aggtcgcgcc cggcctcgtc acggccctcg
    31261 tccagggatc ggacctctac gagatcgaga tcaccgtcga cagactcgcg gaggcgaagt
    31321 ggcgggcaat ccggtcggag tgcgcgggca aggtcgactc tctcgtcgag ctcctcgacg
    31381 ggcgcctctc gcaggcggtg atgtccgtcg tgacccggcc cggagcggga ctcttcccct
    31441 cgccggcgga gattcggatg cgctgctcct gccccgactc cgcgcgtctc tgcaaacacc
    31501 tcgcggccgc tctctacggc atcggcaccc ggctcgacca ccgccccgag ctcctcttcc
    31561 tcctccggca ggccgaccac ctcgagctcg tcaccgccgc cgtcgacgcc ccgatcgcgg
    31621 gcccggcctc tcgggacgaa gccctcgccg gcgccgacct ctcctccatc ttcggcatcg
    31681 agctggagga ggcgacgagt gaggcgaagg cgcccgcccg cgcgcgaaag ccccgctcca
    31741 cggcgacgcc gagagagacg cccgaagcgg cggcggtcct cgcggccgtt ccgaagctga
    31801 agcgcggcaa gccggccgga gcccgcctcg cgaagatggc gaaggtcgcg aaggtcgcga
    31861 aggagatgac caccgccgac ctcgtcgccc tcggcgtctc tcgcgccacg gtccgcaact
    31921 ggctcgccga gcgagtcctc gagccgaccg accgccgggg cgtctacgcc aggacgtctg
    31981 agacgacgcg ccggatcaag gcctatctca gccgtcgccg gaagggctcg tgaaggggag
    32041 ccgtgctgtg tgcagggcca tcggctgaca cctcgcccgc acgcccggtc ctggcgacgt
    32101 gacggtcgga cgggaagacg gcaccggggc gccgcgcgga ttgccgtcac gctggctccc
    32161 gacgcgacaa gcgggagaag gcgacgcaga cggtcctgga gcaggtcgag gtgcttccgg
    32221 aggcgtggag agaggagtga ggggccgggc ttcaggcccc ggcggatgct cccccgatga
    32281 ccccgatccc cagcttccga aggagcgaga ggagccgtcg gtcgaaggtg aggacccggc
    32341 agccctcgcg tcgggcgagg acggcgtaga ggaggtcgta gaccgggtgc ccgtgacggg
    32401 aggcctcgga gagagcctcg acggccagct cgtggtctgg acagagtcgg ctcgggaggg
    32461 ccatcgccat ctcccagcgt tcggcgagga cctccgccgg gagcaggccc ttgctcccga
    32521 gcttccacag ggcgttcgcc gtctcgctcg cgaagaggga cggggcgagg acgagagagg
    32581 cgcgctcgag agccgctaat gcctcctcga taccgggcga tgcgagaacg acgcggagcg
    32641 ccgccgaggc gtcgaggacg atccggggcg ccgtcaccgg gcccggtcct cgcggacgag
    32701 cttctcgagg gaaacacgag ggggccgcgg cttttcctcg cgaaggcgtc gcctcacgtc
    32761 cgcgaggacg agcttccggg ccgaaccgtc cgagagcgtc ttgcgaagct cggtcacggc
    32821 ctgctgggcg aggcttcgct gctcgagccg tgcccggagc gcgagggcct cgtagagctc
    32881 ggcgggcagc tcgcggatct ggagggacga catcggacgg gttccttccg ccttcatcat
    32941 aacgcatgca gaatgaagat gatgaggcgt catgcggagc cgggacgcga acgcggtgcc
    33001 ggagccgctg ccgccggcgg gccgggcgaa gggcggcgcc cccctcaccc caggaagacg
    33061 aaccgcaccg agaggtagat cccgacggcg acgaagacga cgccggtgat ccgcctcgcc
    33121 cagacctcga acctccccac cttcgtcatc gccgcgccga gcgccttcgt cccgcccgtc
    33181 gcgacgaggg ccaggacaac gaccgggagc gccgtgccga agccgtagac gacgggaagg
    33241 acaaccttcg acccctgctg gagcgagagc gggacgaggc ttccgaagaa gagggcggcc
    33301 gagacggggc agaaggagag tgcgaagagc gcgcccagga gcgccgcgcc ccaggcgccc
    33361 ccgtcggccg ccttcgtccg cagctcctcg ctcccggcgg tcgtcgagag cccggccgag
    33421 aggaggccga ggaggtatat cccgacgagg acgaggagcg gcccgagcag cttgttcatg
    33481 taccgctgca ggaactgcga gaggccgggg accgagagga ggccgaggac gacgacggcg
    33541 ctgacgacgg cgtaggcgag ggcgcggccg agggagtagg tgaaggccga gaggaggacg
    33601 aggcggggcc tgtcggcgcg gcgcgccagg tacgacaccg cggcgatgtt cgtcgcgagc
    33661 gggcaggggc tgatcgacgt gaggacgccg agccagaggg ccgtcgcgat cgccggcgcc
    33721 aggtcgggcg tcacgccttc tccaggtagg cccggacctc ccgctcgacg tagtcctgga
    33781 acgcctccgg gtcgcggagg agcggccaga cctggtcgag gttcttccag cggagctcct
    33841 tgccggcctt cacctcggag acgacgaccg acttcgtgaa gaggccgtag tccttcatgt
    33901 agtgggcgtt cgcgggctcg tcggtgttca cgaggcgcca gacgacgcgc ttctcggcga
    33961 tcgggtcggc gaatcgcatc gtcagggtgg cgttcgtcag ctcctcgatc ttcaggcacg
    34021 acaggcaacg cgccgtcgtg tggaagtagg tgacgacgac cgtggacgcc tccgcggccg
    34081 gcggcaccgg tatcgaggcc ggagcggccg ccgaggcctc caccgcgcgc gcgtgccggg
    34141 tttctttcac cacgagggtg attcccgtga cggcgacgaa ggcgaggagg gctccggtga
    34201 cgaggtgctt cttcccgatg ctcatgacgc gaacgctccg aagacgaggc cggtgacggt
    34261 ggacatgacg atcacgaggg agacgtaggc gacggtcttc ttcgtgccga ggtacgagtg
    34321 gatgacgatc atgctcggca gggagagcgc cggaccggcg aggaggaggg cgagagcggg
    34381 tcccttgccc attcccgagc cgaggaggcc ttggaggatc gggatctcgg tgagcgtcgc
    34441 gaagtacatg aaggctcccg ccaccgaggc gaagaggttc gcggcaaggg agttcccgcc
    34501 gaccgccccg gcaatccacg cgtcgggcat caggccggag tcgtgcccgg ggcggcccat
    34561 gagccacccg gcgacgagga cgcctccgag gaggagcggg aggatctgct tggcgaactc
    34621 ccaggtcccg aggacccaga gccggagctc ctccttgtcg taccagcgga tcatcatgaa
    34681 gaggagagcc gcgagggccg cgccggcgag ataccagtgg acctcgtaga ccgccgcgaa
    34741 gaagccgacg gccctattcg aggcgcccca gttcgcgaag acgaggatcg cgaccatcgt
    34801 gccgaggaag gaggcgtcct gccacccttc gcgcgtccgg ccctcggcgg cggctcccgc
    34861 cgcggcgaag gggtcggggc cgttcttcac ccgctcggcc tcctccttcc ggaagatcgc
    34921 cgccatgatc aggccgatga cgacggcgaa ggcgacggcg ccgagcgcgc gggcgaggcc
    34981 gatctgcagg cccagcacgc gcgtcgtcag gatcatcgcg aggacgttga tcgccgggcc
    35041 cgagtagagg aacgccgtgg ccgggccgag tcccgcgcct cgtttgtaga tgctcgagaa
    35101 gaggggcagc accgtgcagg agcagaccgc gaggatggcg ccggagaccg agccgacgcc
    35161 gtaggcgacc gggcgtttcg cggtagcgcc gaggtacttc atgaccgcgc cctggctcac
    35221 gaactgaccg atcgccccgg cgatgaagaa cgccggaacg aggcagagga ggacgtgctc
    35281 gcgcgcgtag tcgcgcaaca ggacgaatcc ctcggggagc gcccgctgca cgcgcacgct
    35341 ctcgatcggc aggaaccagg cgccgaggaa gaccccgacg atcaggagga gcttcgtcct
    35401 ctccttcatg gatggcctcc gggcaggcgc cgcgctcgcc gcggtcccgc cccgtcgcgg
    35461 ggtgtcagag cgcggccagc gccgcgcgga gctcctcggc cttcgggatc cggccggaga
    35521 agagcacctt gccgtcgacg gcgacgccgg gcgtcgcgag gaggccgagc tcgatcatcc
    35581 gctccatccc ctcgaccttc tggacggtga aggggagctt ctccgtctcg acgacgtgcg
    35641 cgacgacgcg gtacgtctcc ttgcagcggg cgcagccggg gccgaggact tcgatcttgc
    35701 ggggggcgga ttccatggcg gggtccttcc ttcgggtgac gaggtccggc gggccggccc
    35761 tcgattcggt cgatcacgaa tcatagttca ttgatggatg agcgtccagg acggaggtga
    35821 gagggacggc gggggcggcg cgggtcggcg gctatcatcg ggtcgtgagc ctgcgcagaa
    35881 cggcctcccg gagcgtcgac gtggcgccgc gaccccggtt cctctcgccg tcggccccgc
    35941 cgcgatgaac cgccgcacgt tcctcgccgc cctcgccgcg cttcccgccg cgcgtcttct
    36001 cggcgggagc gccgccgcga ccgcgcgcga gatgcgcgtc ttcgggaccc tcccgcggcc
    36061 cgaccgcgtt cggcgcgtct tcgccgccgg ccctccggcc gccgtcctcg cgcacgtcct
    36121 ctccccggag cggctgatcg gctggccgat gccgctgccg cccgaggcgc gcgccctcct
    36181 cgcgcccgcc gttcgcgagc gccccttcct cggccggctc tcggggcgcg gcagcacgat
    36241 tccgctcgag aagctcctcg cgctcgagcc cgacctgatc ctcgacaccg ggacggtcga
    36301 cgcgacgtac ctctccgccg cggagaacgt ccacgagcgg acgggcatcc cgtatctcct
    36361 cgtcccgggt cgtctctccg acagcccgtg gcagctctcg gagacgggtc ggctcctggg
    36421 cgtgccggag cgcggcgccc acctcgcgcg cgaggcggag cggttcctcg ccgaggcgcg
    36481 cggatcgcgc cgcgacgggg tacgggcgcc gggcgtctac gtggcccgtg gcgcggacgg
    36541 tctcgagacc gtgaaggagg gctcgctcaa cgcggagtcg ctcgccctcg ccgggggacg
    36601 aagcgtcgcg accggcctcg ggggcggagt cgcacgggtc tcgttcgagc aggtcgcggc
    36661 ctgggcaccg gaggtcgtcc tgacgcagga cgcgggcttc ttcgcgagcg cgcggaggga
    36721 cgatcgctgg cggcgcctcc ccgccgtccg cgccgggcgg ctccatctcg tgccgagcct
    36781 tccgttcggg tggctcgacg ggccgccggg cgtcaaccgt ctcgtcggcc tcccgtggct
    36841 cgcctcgcgc ctccacggca cgcccctcgg aaaggacgag gtcgagcggg tccgcgcctt
    36901 ccacgccgcc ttctacggct tcgcgcccga gcggacggcc ctcgagcggt tgctcgcggg
    36961 ggcctgatgc cgcgctggcc ctggctcctc ctcggactcc tcctcgctct cgcgatcctc
    37021 tccggcctcg cggcgggccc gatcgcgctc ggccccggcg agctctttcg cgcgctcctc
    37081 ggcggcccct ccgcggaagg gcaggcgacg atggtcgtct ggagcctccg tctcccgcgg
    37141 ctcgccgcgg ccctcctcgt cggcgcggcg ctctcggccg cggggacggc ctaccaggcg
    37201 atgttccgca acccgctcgt ctcgcccgac atcctcgggg cctccgcggg agcgggtatg
    37261 ggggcggccc tcgccatcct cctcggcctg ccgacgctcg gcatccaggg agccgccttc
    37321 gccggcggcc tcgccgccgt cggcctcgtc acgctcgtcg cggggcgcgt gcggcggcac
    37381 gacccggtcc tcgtcctcgt cctggccggc atcgcgatcg gaacgctctt ctcggccgga
    37441 atttcgctcg cgaaggtcct cgccgacccg accgtgcagc tcccgtcgat cacgttctgg
    37501 ctgctcgggg ggctcagcgc ctcctctccg gccgacgtgt ggcgggccgc gccgctcgtc
    37561 ggggcggggc tcctcccgct tctcctcctc cgtcaccggg tcgacctgct gacgctcgcc
    37621 gacgaggagg ccgccgcgct cggggaggac gtctcccgcc tgcgcttcac gctcatcgtc
    37681 tccgcgacgc tcgtgacggg cgcctccgtg gcgatcgccg ggatcgtcgg ctgggtcggc
    37741 ctcgtcgtgc cgcacgcggc ccgcctcctc gtcgggccgc ggttcgcgcg gtcgctcccg
    37801 gccgcgctcc tcctcggcgc cgcgttcgtc gccatcgccg acacgctcgc ccgcagcgcc
    37861 gccgcgatcg agctgccgct cggcatcctc acggcgctcg tcggcgcccc cgccttcctc
    37921 tggctcctcg ctcggggagg ccgcttctcg tgacgaagct cgaggcgcgc ggcctgagct
    37981 tcggctacga cgggacgccg gtcggagaag gagtggacct cgacctgacg ggaggcgagg
    38041 tcgtctgcct cctcggcccg aacgggggag ggaagacgac gctcttccgg acgctcctcg
    38101 gcgtcctccc gccgatcgcc ggcgaggttc gcgtcgcgga gcggccgctc tcccgctggc
    38161 cgcggcgcga gagggcgcgg ctcctcggct gggtgccgca ggcgcacgcc ggccttttcg
    38221 ccttcaccgt ggaggaggtc gtcctgatgg ggcggacggc ccgccacggc cgctacaccg
    38281 ccccgtcgcg gcgggaccac gacgtcgccc gcgcggcgct cgagcgcctc gggatcgggc
    38341 ggctcgccga gcgcgtctac acgcagatct ccggcggcga gcgccagctg accctgatcg
    38401 cgcgggcact cgcgcaggaa gccgggatcc tgatcctcga cgaaccgacg gcgagcctcg
    38461 acttcggcaa ccagctccgc gtcctcgggg agatccgccg cctccgcgac gacgacatcg
    38521 gcatcctgat gtcgacccac cagcccgagc acgcgctgga ggtcgccgac cgaatcgcgc
    38581 tcctcgatgg aggacggatc gtcgcgcagg ggccgccgcg tgagaccgcg acgcccgagc
    38641 gcctcgcgca cctctaccgg gccgacctcg cggcggtgac ggcgcggatc gcgccgtcgg
    38701 gggcgcggcc ttgagcgcgc ctccgccgcc aaccgaggag ctcgtcgcgc ggtggggctc
    38761 gcttcccatg ggctccctcg ccctcctgac cggcgggcgg ggggcgggga agacgcgctg
    38821 gtgcgaagcg ctcgcacgcg cggcgcgcga ggcgaagctc gccgtccgcg gcgtcctctc
    38881 gcctcccgtc ctcgcgggcg gcgagaagcg cgccatcgac ctcctcgacc tcgccacggg
    38941 ctcacgccgc ttcctggccg agcgggcgcg gccggacctt cccgggaccg agggcctcgg
    39001 ctggcgcttc gacccggagg cgctcgccgc tgggaacgtc gcgctcgaga gggtcggggc
    39061 gtgcgacctc cttctcgtcg acgagctcgg cccgctggag ctcgggcgcg ggagcggctt
    39121 caccgcggcg ttcggcctcc tcgacgcccg ccgccacggc ctcgcgatcg tcgtcgtgag
    39181 gccgtcgctc gtcgccgcgg cgggggcgcg ctggcccgga gcgacgcggc tctgggacct
    39241 cggaggagat cgatgacgga cgttgacggc aagcgccccg agacgatcct cgacgtcgac
    39301 ttcgggcgcc tctaccggca gcacttcgcc gcgagcggcg gacgcgagaa gccgcccgag
    39361 gcgtgggacg cgcgcgcttc cgacctgagc cgcgagctgg aagggagccc ctacgtcgag
    39421 gagctcctcc gcctcgtcga cctgacggac tgccgcacgc tcctcgacgt cgggtgcggc
    39481 cccgggacga tcgccctcgc tctcgccccg cgcctttcga aggtctgggg cctcgacttc
    39541 agccggggga tgctcgacgc gatgacgcgt cacgccgccg cgcgaggcct cgcgaacgtc
    39601 gagccgatcc tccgctcctg ggaggagccg tgggacgacg tcccggcgtg cgacctcgtc
    39661 ctcgcctccc gctcgacgct cgtccgcgac atggaggacg ccctcgcgcg gctcgacgcg
    39721 aaggcgaggc ggcgcgtctg cctgacgagc ctcgccggag ggcgcttcgt ccgcgccgac
    39781 ctcctcgcgc tcctcgggcg cgagaagccg gcctacccgg actacatcta cgtcgtgaac
    39841 ctcctccacc ggatgggccg tcatccgcgc ctcgacttca tccgcgccgg gcgccggccc
    39901 gacgaaaagc cggggttcga tcagctcgcc cggcgcgtgg agttctcgct cggcgctctc
    39961 acccccgagg agcgagggcg cctcgaagcc tggtaccgcg aggaccccgc gcgcgccggg
    40021 accggccgcg agccgtcccg ctgggcgttc gtctcgtggg agaaggagta gcgcggggct
    40081 cgggatccgg acggcgcccc ggaaggacgg cgctcggtgc ggaagcggtc ggcccggccc
    40141 cgaatcgcgg gctcggcgcg gagggggcat actggtgcga ggctggacag tgggagacga
    40201 gacgcccccc tcggctgcgc ccgccccggg cggccccggc tccggccggg cggcggccgc
    40261 cggcttggtc tgcgccgtcg cgacgatggc gcttggcgcc gcaaccctcg tcggttgggc
    40321 gaccggtcgg agggcgttga ccgccttcga cctctcgagc atcccgatgg cgccgagcac
    40381 ggcgctcctc ttcctggtcc tcgggggcgt ggccgcagtg gcggcccggc cgggacgagg
    40441 ggcgggggcg cggagcgcgg gcgtcgttct cgcctccctc gcggcgctcg gctcggctgt
    40501 cctgctcgtc gccaggctgc gcggaacgta cctgtcgatc gagcacgtcg gccttcggat
    40561 cgaggggcgc gtttccggct ggccgctcgg gcacatctcg cccgtggcgg ccgccgcgtt
    40621 cttcctcgtg agcgtctcgc tcctcgcgct ccagatccct ccggggcggg gccggacgcg
    40681 aggatggctc gccacggctg ccggcctcgt agtcctcgtg tgcggcgtcg tcttcttcct
    40741 cgcatatgtg gtgggggagc cgctcctcta ccgtggcccg gtcctgccgc cggccgtgac
    40801 gacgagcacc gcgctcgtca cgatcggcct ggccgtcgtc ctcgccgcgc gggcccggat
    40861 ccctcggccg gacgacgagg tgcgcctgcc gcgcagcgcg gaggcgatgc tggcggggct
    40921 cttcctggtc ttcgcggcgg gaatcgtcct ctggggtgcg acctggttcc gcgagcaggg
    40981 ccggcgcctg cgggagcggg tcgacctgca ggtctcgacg atcgcggagc tcaaggcgac
    41041 ggacctcgcc cgctggcgga ccgagcggct cggcgacgga cgggtccttc aggagagccg
    41101 ggccttccgc cgtctcgtcg acggtctcct cgtcgggagg ctcacgagcg acgacgaggc
    41161 ggtcctcggg cagtggctcg gcagcgtccg ggagagctac ggctatgcga gcgtcgaggt
    41221 cctcgacgct cgcggggcgc ggcgtctttc gatcccgaac gacgccgcac cgatcgtggg
    41281 tgacgacggc cggcacttcg ccgagctcct ccgatcgggc gaagcgcgtc tcgaagacct
    41341 tcacacgcac gagccacggg gcgtgctccg gatggcggtc gtcgtcccga tcgtcctcga
    41401 ggggcgtccc cggggcgccg tgctgctcgg gatcgacgtc cggtcccacc tcgagacgtt
    41461 cctctccgac tggccggtcc cctcgcagac ggcggcgacg tttctcgttc gccaggacgg
    41521 cgacgcggtg ctcgtggcgc gggccgcgga cgcggacggg aaggagccgc ggatcgagcc
    41581 ggtcccgaag gcgcaggtcg aaccgatgtt cgtgcgcgcg gccgccggcg aggaggtggt
    41641 cgggctcttt ccccgccccg acggaagcca ggtcgtcgcc gccgcccgcc gcgtgactgg
    41701 ctcgccctgg aagctcgtcg catccgtcga cgtcgaggag gcctacggcc ccctgcgcga
    41761 acggctctgg ctcacggtcc tcgcgatggc ggggctgctc gcggcggtgg ccgcgggcgt
    41821 cctggcggtc tggcgccgcc agctgggccg ggccgagcgc gggcgaaggc tcgcggagcg
    41881 ggagggcgcc tcgctccgtg aggtcatcga acggagcctg aacgagatct tcgtcttcga
    41941 cccggtcacc ttccgctttt ccttcgcgaa ccggggagcg gcgcggaacc tcggctactc
    42001 cgaggaggag ctgcgcggga tgacgccgat ggacctcgtc ccgggaatga cgcccgacga
    42061 tttcggcgcg atgttccggg agttggatga gtcgcccggc gaggtctaca ggttggagac
    42121 cgtccaccgc cggaaggacg ggagcgtcta cccgatcgag gtcaacctcc agcgggtgag
    42181 cgcggccgcc ggcgacgtct atctcgcggt catcaacgac atcacggagc gcaaggccgc
    42241 ggaagggaga atccgccacc tgaaccgggt ctacgcggtc ctcagcgacg tgaaccaggc
    42301 gatcgtccgc gagcgggaca taccgtccct ggccgccgag gcgtgccgga tcgcggtctc
    42361 ggtcggggga ttccacgatg cacggctctt cctcgcggaa gacggtgcga tccggagcgt
    42421 cggccgggcg ccggccgcgg agccggaccc gtccgcgggc gagtcggcgt gggccgagac
    42481 gctcgccctc gagtcgatcc ggcaggggcg gtcggcgacg tcgcgcgcgg gagagacgac
    42541 ggacgcagcg gcaagcgggg ccgcgcgcga ccgcggaccc gccgtcgcgc tgccgctcgt
    42601 cgtggagggg cgtccggccg gcgccttcgt cctggcggca ggcccggacg accacctcga
    42661 cgtcgaggag atgaagctcc tggaggagat ggccctcgac gtcgcattcg cgctcgagac
    42721 gatccgcggg gaggccgccc gcaagcaggc cgaagccgag gtccggcgcc tgaatgccga
    42781 cctcgagcgc cgcgtcgagg agcggacggc ggagctcgcg agcgccgtcc gcgagctcga
    42841 gacgttcgcc tattccgtct ctcacgacct gaaggccccc ctgcgggcga tcgacggcta
    42901 cagccacctc ctcgtcgagg agttcggccg ggcgctcgac gggaaggcgc gggactacct
    42961 cgagcgggtc cggggcgggg cccggcggat gggcgagctg atcgacgccc tcctggccta
    43021 ctcgcgggtc gagcggaagg ccctccagcc cgagtcgatc acgctcggcg agagcgtccg
    43081 gctggcgctg gcgcacgtcc aggaggaggt cgcttcgacc ggcgcctccg tgagcgtcga
    43141 cgtcgacggc ctcgtcgtgc gcgccgatcg ggaggggctc gcgatcgtct tccgcaacct
    43201 cctcgggaac gcgctgaagt tccgaagggg cgcggaggcg ccgcgcatcg agatcggcgg
    43261 cgagtcgtcg ggagggacct gccgcttctg ggtccgcgat cacggaatcg ggttcgacac
    43321 ggtgtacgga gagaagatct tcgagatctt ccagcggctc cacaggcagg aggagttccc
    43381 cgggaccggc atcggcctgg ccctcgtgag gaaggccgtc gagcgcatgg gcggctcgat
    43441 ccgcgccgag agcgagcccg ggcacggcgc gacgttcttc gtcgagctcc cggggggcgc
    43501 gagcggcatg tcccccggcg ctggagcgcc gggggccgaa aggacgtccg ggagcggggg
    43561 ctagaaggag acctgggccg cgacgacggc ctggaggtac ttctcgtcga ggacgccgcc
    43621 gacggagcgg ccgtcgtcct cgatcgactc gacgttcagc ttcaggttcg cgttgttccc
    43681 cttgatgaag tagtggagcc cgccggtgag gatcgtgagc ttgttgtcgg tggcggattc
    43741 cttgttccgg tcgaactgct cgtagcgggc ggcgagctgg agcttcttcg cgaaggtgta
    43801 gaggacggtg ccgtaccagc cgttccgctt cagcttcgtc acgacgccgc cggaggcctg
    43861 ctcgtcgcgc ccctcggcgt actcgaagcg gaagccgagg gggagctctt cggtcccctc
    43921 ccagcggacg tgcgcggcgt agcgctcgcg gccgaggtag gcggcgccct cgccggcgct
    43981 gtagccgccg gaggcgccga cgacgagccc cttcgtcggc ttcacgtcga ggcgcgcgtt
    44041 gaagttgagg gcgtcgttgt tgtcgttgtt gttgagcgtc gtcccctggg tgaccgagac
    44101 gccggtcgtc accatcgggc tccagttggc cttccagaag aggccgagct cgcgcttgtc
    44161 ggcgaactgg cgggcgacga cggagcgctc ggcgaagtcg agggccgagg aggagcggtt
    44221 cccttcctcc atgagccaga ccttcttctg ccccagctgg aggtcgtggc ccttgaggcc
    44281 cgagtaggag atggcgaagt cctggaggat cttgccgtcg gcgccggtgg tgacgctcgg
    44341 gctcttcgtc gggtcgatca tgacctcgaa gccccactcc ttcgtcacct tgcccgagag
    44401 cttgatctcg gcgcggcgaa ggcggaacgt gttgacgccg gtggagttgc cgagctggct
    44461 cgagccgctc ccggtggccg agtcgtccat gacgtaccag ccctggagga ggccgctgaa
    44521 cttgagctcg ccgtccccga tcttgagggt cgtgggcggg accggcttcg ggggcttcgg
    44581 cgcggaatag ggacggacct cgggctcctt gccgtgcttg aagacgtaga ggtcgagggc
    44641 cgcgtcgttg tcgaagacga ccgtctcgcc gttcgggccg aagccgatct tcgtgatcga
    44701 cttcccgagc tcgcccgagc cttcccaggc cttgaactcg ttcgggtcgt tgaagacgta
    44761 gatccggccg tccttcgcga cctccttgta ccagaggagg ttcacctggg ccggggcggc
    44821 gccgacgagc aagagaaggc ccgccagggc gattccgtgg acgcgtcgaa gcatggggct
    44881 cattccgtct ctcctttcag atccgtccgg gaagggcgat gtctccgagg gccgccgaag
    44941 cggtcgcgcc tgcggggacc gtccgggccg ggcggagtct cggaagcgga cgggtgggcc
    45001 ccgtggagac ggcatgaagg tcgtgtaagg agagggggcc ccggccgggg cgcttcacac
    45061 ggagttaacc tggccgtaac aacgtcccgt tcgccctcgg gaagactccg cctcggtttg
    45121 gaatcccgaa ggaggacgca gatgaagatg accgccaccc tcgccgccgc cgtgctgctc
    45181 gccggcacgg ccgccgcgca gacgatccag gtcgacccgg cgatcaagcc ctacaaggcc
    45241 gttgccgggg tctcggggaa cgtcagctcg atcggctccg attcgctgaa caacctgatg
    45301 acgctctggg ccgagaagtt caacaagatg tacccgaacg tgaagatcca gatcgagggg
    45361 aagggctcct cgacggctcc gccggcgctc atctcggcca ccgcccagct cgggccgatg
    45421 tcccgcgcca tgaaggggag cgagatcgac gagttcgaga agaagttcgg ctacaagccg
    45481 acgcaggtcc gcgtcgccgt ggacgcgctc gccgtcttcg tcaacaagga caatcccgtc
    45541 gcctgcctga cgatgccgca ggtcgacgcc gccttctcga agtcccggcg ggccggctac
    45601 aaggagaacg tcaagacctg gggccagctc ggcctgacgg gcgactgggc gaatcgcccg
    45661 gtcagcctct tcggccgcaa ctcggcctcg ggcacgtacg gcttcttcaa ggagcacgtc
    45721 ctgaagaacg gcgactacaa ggacgaggtg aaggagcagc ccggctcggc ctccgtcgtc
    45781 cagggcgtca ccgtcgaccg gttcgcgatg ggctactccg gcatcggcta cgcgacggcg
    45841 ggcgtcaagg cgctcccgct cgcggcgaag cagggcgaca cgtgccaccc ggcgacgccc
    45901 gaggaggcct actccggcaa ctacccgctc gcgcgcttcc tctacgtcta cgtcaacaag
    45961 gcccccggca agccgctcga cccgatcgtc ggggagttcc tcaagctcgt cctctcgagg
    46021 gacggccagg aggtcgtcgt caaggacggc tacttcccgc tcccggcgaa gatcgagcag
    46081 gaagagctcg ccaagctcct gaagtagagg acccgacgtg cccgccacga ccgagagcgt
    46141 cgccgcagga cggcccccgg cgcccgcgag gacgacgggg gcggcccgcc cgctcgggcg
    46201 gcggctcctc gcggatcgct tcgccgcccg gttcgtcacg ctctcgggcg tggcgatcat
    46261 cgcggcgatc ctcgcgatcc tcttcgtgat cgtggcggtc gtctggcctc tcttcgttcc
    46321 gccatccgcc cgggcggtcc cggccgggag cctcccggcc gggaccgtcc ctctcgccct
    46381 ctccgtcgac gaataccgcg aggaggcgtt cgtcgcgacg aaggcgggcc tcgtcggcct
    46441 cccggtcccc ggtggagagg tcttcacgcg ggagctcccc ttgctcggag acgcgacgat
    46501 cgtgaaggcg ggcgccccgg agccggagct gatcgttctc ggcctctcgg acgggcggct
    46561 ctaccccgtc gaggtctcga cggaggtcgc gtacgaggac ggccggcggc gggtgacgcc
    46621 ggtcgtccgc cccggggagc cggtgacggt gactcccgac gggaagcccg tcgaggtctt
    46681 caagtacgtc cgcggacccg acggcccgat cgcgctcgcg gcgacgggtc cgcgggagat
    46741 cactctcgtc cgggtgaccg agaagacgag cctcatgggc gagacgacgc gcgagacctc
    46801 ctcgctcgtc ctcccgatca gagcccgcgg agtcatccgg acgctcgcgc tcgacgttcg
    46861 cgggcaggac gtcttcgccg ggacgtcggc cggggagatc gtccggctcg acctgcggga
    46921 cgagcagagc ccgaagcagg aggagacggt cccggcgtcg gccacgggcc gcgaagtgac
    46981 ggtcctcggt tttctgaacg gcgaccggac gctcgtctcg ggcgacgccg aaggccgggt
    47041 cgcctcctgg cacgtcgtcc cgtccgagac cggaaacacg cgccggctga cgcggatcca
    47101 cgacttccga agccacggcg ccgcggtgac cgccttctcg ccgtcgcgga gggacaaggg
    47161 cttcgtcacc ggggacgccg cgggcggcgt cgcggtccac tacgcgacga ccgggaagac
    47221 gctcctctcg ctctccgccg ggaccgcgcc gctcgccgcc gtcgtcttcg cgccgaaggc
    47281 ggacggggtg ctcgcggcgg ccgcggacgg ccggctccac cacttcgagc tgaagaaccc
    47341 gcatcccgag gtgacgtggc gctcgctgtt cgggaaggtc tggtacgagg ggtacgcccg
    47401 gcccgactac gtctggcagt cgacgggcgg cacggatgac ttcgaggcga agttcagcct
    47461 gacgccgctc atctacggga cgctcaaggg gacgttctac gccctcgtcc tcgcgatccc
    47521 gctcgcgctc gcggcggcgc tctacaccgc gcagttcatg cacccgagcc tccgctcgtt
    47581 cgtgaagccg gtcgtcgagg tgatggccgc gctcccgagc gtcgtcctcg gcttcatcgc
    47641 cggcctctgg ctcgcgccga tcgtcgagag gatcgtcccc gggctcctcg tcatgcccgt
    47701 cgtggtcggg gcgttcgccg ccctgggcgc cgtcggctgg gggaagctgc cggcgcgcgt
    47761 ccgggcccgg gtgccggcag gctgggaggc gctgctcctc ctgccgctcg tcgtggcggc
    47821 cggcgcggtc tccctctgga tcggcgcggg cctcgagcgc tggctgctgg gggccgacta
    47881 ccggcacttc ctcctcgagg cgctcgggct cacgtacgac cagcgcaact cgctcgtcgt
    47941 cggcctcgcg atgggcttcg ccgtcatccc gatcatcttc accgtcgcgg aggactcgat
    48001 ctcggctgtg ccgccggggc tgaacgccgg ctcgctcgcc ctcggggcca cgcgctggca
    48061 gacggccctc cggacggtcc tgcccacggc ctcaccgggg atcttctccg cgatcatgat
    48121 cggcttcggg cgcgccgtcg gcgagacgat gatcgtcctg atggcgacgg ggaacacgcc
    48181 cgtcatggac ctttcgatct tcaatggctt ccgcgcgctc tcggcgaaca tcgccgtcga
    48241 gctgccggag gcccccgagg gagggacgct cttccgcgtc ctcttcgtcg cggccctcct
    48301 cctcttcgtg atgacgttcg tcgtcaacac ggtggccgag ctcgtgcggc tccggctccg
    48361 gaagaagtac aggaccttcg aatgagcgcg tccgagggga gaccgtccct cgcgcggaag
    48421 agcggcgtct tccggagcgg ggagcccttc atctgggtga ccgggagcgg cctcgccgtg
    48481 gccgtcctga tggtgttcgg cctgctctgg ctcgtcctcg cgaacggcct cggcttcttc
    48541 tggccggccg acgtccacct cttcgcgctc gaggacgggc gcgccctcgc cgggcgcgtg
    48601 gcggcccggg agaggatccc cgaccccgat gccgcccccg ggacgccgga gcgctggcgc
    48661 atcaaggtca agcaggggaa ccgcgacctg accggggagg acttcgtctg ggtcgacgag
    48721 tccgccgtcc ggtcccggac gacgcccgcc gacgtcgtcg tcctcgagcg gcgggagtgg
    48781 ggcgacttct acggctgggt gagcgaggtg cgggacgggg agacgctcct cgcctcgggg
    48841 cgcgaggcct cgtggaagaa ggtcgtcgag ctcctcccgc gggcgcgcga ggtccacgag
    48901 gaggcgctcc ggctcgagaa aggccccatc ggggccgtca acttcgagaa ggagaagctc
    48961 cgcctcgagc ggaagaagct cgagctcgaa ggggcggcgg ccgggtcgaa gcggaacggg
    49021 ctcgacgcgg aggagcggga gctccaggcc gagtacgacc ggctcttcgc agcgcttcag
    49081 gaggtccggg ccggggtgaa ggccgtcctc gtcctcgaga ccgcgggagg ccagacgaag
    49141 gagctcccgc tctcgcaggt cgtccgggcc gaccgcgtga acgggatggg gcggctcgcg
    49201 aaggcgggac gctacgccgc gaacgtctgg gccttcgtgt ccgaggaccc gcgcgagtcg
    49261 aacaccgagg gcggggtctt cccggcgatc ttcgggacgg tcctgatggt cctgatcatg
    49321 agcgtcgtcg tgacgcccct cggcgtcctg acggccttct acctccgcga gtacgcgaag
    49381 caggggccgg tcgtcagcgc ggtccggatc gccgtgaaca acctcgccgg cgtgccgtcc
    49441 atcgtctacg gcgtcttcgg cctcggcttc ttcatctact tcgtcggcgg gtcgatcgac
    49501 cgcctcttct acccggaggc gctcccggcg ccgacgtggg ggacgggagg gatcctctgg
    49561 gcgtcgctga cgctctcgct cctgacggtc ccggtcgtca tcgtctcggc ggaggagggg
    49621 ctcgccgcga tcccgcgcgg gatgcgcgag gcgtccctcg ccctcggggc gacgaagttc
    49681 gagacgatgt ggcgggtcgt cctgcccgcg gtcatgccgt cgatcctgac cggcctcatc
    49741 ctcgcgatgg cccgcgcggc cggcgaggtc gccccgctca tgatcacggg cgtcgtgaag
    49801 ctggcgccgt cgctcccggt cgacggcttc gcccccttct tccacctcga ccgcaagttc
    49861 atgcacctcg ggttccacat ctacgacgtc gggttccagt cgccgaacgt cgacgcggcc
    49921 cgcccgatgg tcttcacgac gacgatcctc ctcatcctca tcgtcgtcgc cctcaacctc
    49981 acggcgatcg tcatccgggg acggctccgc cggaagctcc gctcctccgg gatctgaggt
    50041 acgtcatcat ggaagcccag acgatggccc cgcccgcatc cgtcccgccg ctcgagctcc
    50101 ggaccgcccc gggtcccgaa ggcgccgccg cggaggcgcc gccgctgatc gagctcgaga
    50161 agttcagctt ctggtacggg gagaagcagg cgctcttcga catcgacctg aaggtccgcg
    50221 agcgctcggt caccgccttc atcgggccgt cgggatgcgg gaagtcgacg ctcctgcgct
    50281 gcctgaaccg gatgaacgac ctgatcgacg gcgtccgcca ggaggggtcg ctccgggtcg
    50341 ccgggcagga cctctacgcg ccggacgtcg acgtcgtgag cgtgcgcaag cggctcggca
    50401 tggtcttcca gaagtcgaac ccgttcccga agtcgatctt cgagaacgtc gtctacggcc
    50461 tccggatcca gggggagagg aacaagaccg tcctgcgcga cgcgtgcgag tcggcgctcc
    50521 ggggcgcggc gctctgggac gaggtgaagg accgcctcga cgaaagcgcc ctcgcgctct
    50581 cgggcgggca gatgcagcgc ctctgcatcg cgcgggccct cgccgtggag ccggaggtcg
    50641 tcctgatgga cgagccctgc tcggccctgg acccgatcgc gacggccaag atcgaggagc
    50701 tcatcttcgc cttgaaggag cgcttcacga tcgtcatcgt cacgcacaac atgcagcagg
    50761 ccgcgcgcgt ctccgacgag accggcttct tcatgctcgg caagctcgtc gagttcgacc
    50821 ggaccgacgt catcttcacc cggccgaagg agaagatgac cgaggactac ataaccggga
    50881 ggttcggttg atggagcggc ccgcggccgc gaggcctgcc gagacgccgg gcggcgcgcc
    50941 ggcgcgctgg cggatcgccg tcgtcgagga cgacgaggac ctcgccttca cgctcgtcta
    51001 cagcctccg
//
LOCUS       DAOONL010000018        49442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_495_length_49442_cov_20.840120, whole genome shotgun sequence.
ACCESSION   DAOONL010000018 DAOONL010000000
VERSION     DAOONL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 49442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49442
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_49442_cov_20.840120"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PLP50_04735"
     CDS             <1..1173
                     /locus_tag="PLP50_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50890.1"
                     /translation="VAVPGPYAAFVAHRALDAARSVFLFSDNVPLADEVALKRRAAAL
                     GLLVMGPDCGTAILAGVGLGFANRVERGPVGIVGASGTGIQELCSLLDRSGVGVSHAI
                     GTGSRDLSAGVGGLMTEAGLGLLANDPATRALLLVAKHPDAGVAERLHGLLATLGKPV
                     AVRYLGEGRRGEVDGVLYAGSLDEAAEAAASRVASTPAARGDLAFAAREAVAPVGAGG
                     SRLVGLFGGGSLAAEARCVLEARGIATRVPEEKLAANAPLPPGNLVVDTGDDAYTVGR
                     PHPMVDQTVRCELIRAAGSDPSVGVLLLDLVLGDGAHPDPAPEIVEAVAAARAARGSR
                     PLSVVASVCGTRRDPQGTARQEETLRRAGVEVEPSAARAAATAADLVLSVRQEVAR"
     gene            1170..2609
                     /locus_tag="PLP50_04740"
     CDS             1170..2609
                     /locus_tag="PLP50_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPA50891.1"
                     /translation="MSATSLLGGPIEVVHAGIDAVAEAPRAAGAPVTVVDFRPPAGGD
                     TRRMALLSELLSGPAAREIDAANARALARLLASRPHLVAIGVARDVVPGMADDLFLHA
                     GPPVTWERMCGPMRGAVIGGLLYEGRAKDADEAERLAASGVVRFDPCHHHDAVGPMAG
                     LVTPSMPVFVIEDRGGERPGTRTFCTLNEGLGKVLRYGAFSEEVVARLVFMRDELGPV
                     LAEALALRGPIDLKHLMAQALQMGDEGHNRNRAGTSLLFRELAPAVVRATADRERAAR
                     VLDFVNGNDHFFLNLSMPMGKCLLKAAEGEPLSSLVSVMARNGTDFGIQLASMPGRWF
                     TGPALNVRGLYFPGRTEADANPDIGDSAITETAGFGGFAMAAAPAIVQFVGGSAEDAL
                     AATRAMRRITLGPNPGFAIPSLGFSGSPTGIDLRLALERNLLPQINTGIAHREPGVGQ
                     VGAGLVTPPWEAFHAALEAFAEHVRASRA"
     gene            2625..3500
                     /locus_tag="PLP50_04745"
     CDS             2625..3500
                     /locus_tag="PLP50_04745"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPA50892.1"
                     /translation="MRPIELSIPLGIGTPAWPTYEPLQMKYFKRLAPNGANGQLLTHS
                     NHLGTHMDGEIHFYSPGKDMAALELDFLMHEGAIVDLSDAVGDYDVYTSKMVEERVEV
                     KDGDILIIHTGYHHYGWDQPEADEIRYMVKHPGPDREFAEWARRKKLRWIGVDCGSAD
                     HPMNTIIRTWMPRQAKEADAHFRAKYGMPLDQFYDDGKYQLMHLELFNHGIIHAECMG
                     GDIDLLLNQRAVIGAFPWRLVDGESCICRILAFVDDDRHAALMAKKEKAAKTKWNDVA
                     GAPNAWLHEEARKKA"
     gene            3526..4989
                     /locus_tag="PLP50_04750"
     CDS             3526..4989
                     /locus_tag="PLP50_04750"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPA50893.1"
                     /translation="MTDLAFRPATALLGALERKETSSEELLRLYLTRIERLGSFHAVV
                     TLDTDRALAEARACDAERARGERRGPLHGLPVTVKDSFETADLRTTSGGTPELMAHVP
                     ARDATVVARLKAAGAIVFGKTNLSTQAMDIQTYNAAFGTTPNPWDASRTAGGSSGGSA
                     VAMSTGLSALEVGSDLGGSIRIPSAFCGVFGHRPSYGIVPTRGHIPGPPGTLAAPDLE
                     TAGPIARSADDLDLALRLLAGPDEGHAVAWRLELPPPRRRGLSEYRLAAWLDDPAAPV
                     DTALRERLEAVIGELRAAGAEVDTEARPGFAFADNARLFMQVLYGSMSAGLPEGQFEK
                     IRARAAALAPEDDSFRARLARDTAQTHREFDLAREERERRRARWAELFERYDALLCPV
                     FPTPAFPHDQSPDFARRTLAVNGAHVPYLGTLLGWPALSGLASLPGTSAPVGFTREGL
                     PAGIQVVGPYLEDRTAIHVAKLVAEVAGGCVVPPGVE"
     gene            complement(5134..5259)
                     /locus_tag="PLP50_04755"
     CDS             complement(5134..5259)
                     /locus_tag="PLP50_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50894.1"
                     /translation="MKDRAAWLRKVRAGLAPGGRVVVVDFVPKSVEERPWGPPPS"
     gene            complement(5311..5997)
                     /locus_tag="PLP50_04760"
     CDS             complement(5311..5997)
                     /locus_tag="PLP50_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA50895.1"
                     /translation="MAAKEVRLRPSRPSRPVAALVLALLASSAAARADGTSPAAPAPP
                     TPLESALAAKEWSRALPLAEEAAEGAGQAFAEALYRVARVRAFLGQRTEALDALERAH
                     GAGLFDASALRKDGAFDALVDDARFQALAKAVWLKGYLWLLERKERDADQKPDEVMRA
                     LALKPGERIADVGAGSGYFTLRLARAVGPSGSVLAVDANADLLAHLDGRLKQAGLTNA
                     RTLLVQKDDP"
     gene            complement(6099..7592)
                     /locus_tag="PLP50_04765"
     CDS             complement(6099..7592)
                     /locus_tag="PLP50_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPA50896.1"
                     /translation="MLGIVERAGNALPHPATIFLLLALAVLGASAIGSWAGLSAVHPG
                     TGKAITVVNLLSVEGLHLVLEKAVSNFTSFAPLGTVLVALLGIGVAEGSGLIGTLLRL
                     LVLSAPRKLLTFVVVFAGVMSNAASEVGYVLLIPLAAVLFLAAGRHPIAGLAAAFAGV
                     SGGYSANLVLGTIDPLLAGLTQEAAHIVDPSYVVSPAANYYFLAVSTFLIAAAGTWVT
                     EKLVVPRLGAYAGTEKADGELRPLDAAEKKGLRNAVVVLALFAAVVLWGVVPEGGFLR
                     DLKTGGVLHSPLLTAIVPFIFLGGALSGIAYGAGAGTMKNDAAVVRAMSKSMETLGSY
                     MVLVFFAAQFVAWFNWSHLGLIGAVKGAELLKATGLGGIPLMLGFVVLSAGVNLLMGS
                     ASAKWAVMAPVFVPMLMLLGYTPELTQAAYRVGDSVTNVISPTMSYFALILATVQRYD
                     AKAGLGTLIATMLPYTLVFLVSWSLILMGWIALDLPVGPGAGLRLAP"
     gene            complement(7789..10008)
                     /locus_tag="PLP50_04770"
     CDS             complement(7789..10008)
                     /locus_tag="PLP50_04770"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPA50897.1"
                     /translation="MRAVRLPTALLALLALAAVCAWRSSPPSPREADAAATEFSAARA
                     AEVLGRLLGDGAPRTPGSPAHAAFRERLLDEVRRLGLTPNVDEGLACAPHGSCARLVN
                     VLAELPGTTGGPAVLLTAHTDSVGAGPGASDDGSGVAVLLEAARALLAAQRRNPVLLL
                     FDDGEEDGLLGAELFARSPRARSVGAVVNLEARGTTGPSLLFETSADNAWLVRRAVSR
                     LPRPVTSSLFAFVYDRLPNDTDLTVYKREGLAGLNLAFIGSAARYHTREDSLANLSRA
                     SLQHQGENVLALARALAETDLLEPPSGRAVFFSVAGAFVLAWPLPAAPAVALLALLLV
                     RLACLRRGGRFLPRIRALVPFLAPFAAALSGVLAWQLLRALGAFPVTFVPRPWASLAA
                     AVAAGLLAALPVLSAEARRAGDAAASWERAWTSTALLGLVLALAWPETSYLLVAPALV
                     AGGARLALPEPVSPWLPFAASGLVLLPLALFLPDALGPAALPVVAAAFGIVLATLPVD
                     STRGAWRRPVGVLAALLVAASAAALALSKPTPDEPEHGTMIAVEEEGRPEARLAYSPA
                     SGRLHPAVAAAGRFEKAPGLYPWASKASPFAAPLPSTSLAFPELSIVDSAPLPDGRRR
                     VRLRLASARRAPRLGLVFPDAAGVEEIRVEGHPLPPPYLRRTPTNGRPLRLSVRGAPP
                     EGFVLELTLPATPREAFVLDETPGLPPSATTIGRSRPATVAPSNGGDATLLFRKVSL"
     gene            complement(10035..11264)
                     /locus_tag="PLP50_04775"
     CDS             complement(10035..11264)
                     /locus_tag="PLP50_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPA50898.1"
                     /translation="MRRTALALAALPVFLASPAAAEERRDGVVFAEKVDAKLDAIRAE
                     VRAKETPAPKKRMWVDFSAVDAPKAVSEFTSVWHKPPVCQGLSGMCWCFSTTSFLESE
                     VHRLTKRELRLSILHTVYWEHVEKAKGWVRSRGTSVYGEGSQPAAVLRALRNQGALPA
                     ESYTGLRDGKREHDHQATLYAEIKAYLDGVKASGAWNEAAVVSTVRAILDHHLGAPPE
                     TVVVDGAAMTPKEYLAKVVRLDPDDYVAFLSTMEQPYWEKAEYAVPDNWWHGKEYVNV
                     PLDAFLAAIKGAVRGGYSLVVAGDMSEPGYSIGPPGIAVVPSWDVPSPFIDEAARAFR
                     FANGSTTDDHGLHVVGITARKGKDWFLVKDSWSSSWNNAHPGYYFFHEDYVKLKMLAF
                     LVHKDAVKELLARVKRS"
     gene            11461..12021
                     /locus_tag="PLP50_04780"
     CDS             11461..12021
                     /locus_tag="PLP50_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50899.1"
                     /translation="MVPLTSLWIPILVSAVLVFVVSSVIHMLLPVHKNDFRKLSREDD
                     ALDAMAKLGLEPGDYMFPLCSSMAEMKDPRFLSKMEKGPVGVLTVMPSGPPAMGPSLA
                     LWFVYCAVVGLFAAYVAGRALPVGAHYLAVFRFAGVTAFVGYSLALWQNTIWYKRSAV
                     STLKSTIDGLVYALCTAGVFGWLWPK"
     gene            complement(12076..12612)
                     /locus_tag="PLP50_04785"
     CDS             complement(12076..12612)
                     /locus_tag="PLP50_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA50900.1"
                     /translation="MPTDTLTPETVLAEWRSGNLEDLVELGVAAARAGEYERGLIFLA
                     EAYRHLSREKHRLSGPLLSSYGLCLALHKGRIKEAAEFCWLGVEKDHFNPDAYYNMAR
                     VWMAGRSRRKAVEALEKGLALDPRHPGLVRLRTEFGSRRPPVISFLHRDNPLNVSLGK
                     VRKRLAAKKTSPAPRSGR"
     gene            complement(12648..13796)
                     /locus_tag="PLP50_04790"
     CDS             complement(12648..13796)
                     /locus_tag="PLP50_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPA50901.1"
                     /translation="MELLLALLLAASVVALASAWSALKTERGRVHRLEDRLAVARPDA
                     TAPSSTPLPEPPPAATPPAPPAAAPRLPAPPEPAPYERAPSFESFAATLLGPAERLGD
                     SMRAADPVLADTAARLRSPLPPSSPVLAGDDPAPPRVDVDGLGRALATAERISDERSR
                     LADDVRSLRSALDRVAELDTGLSRGLSELARTSEALMPLASSVSGLADRANLLGLNVS
                     LLSARAGEAGAPFEEAAAELRSLFEEARRLSRELIEAARRNEGGTRRVSALVEESVGA
                     ATAGQERGARASERLGGLEGLCSQLGRTLEEGVRSTRDAAESARLVRERLDTVRAAAE
                     ARAAEATRLRAEADAARETLRAAAERVEALRLDGGALRAAVQRVTTGA"
     gene            13851..15020
                     /locus_tag="PLP50_04795"
     CDS             13851..15020
                     /locus_tag="PLP50_04795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPA50902.1"
                     /translation="MTTTLLLRAGRDDAARRRHPWIFSRAIAGSPPAGEVEVRSASRQ
                     LLGRGFASPRSSIAARIWRCGEGPLDAPYVRGLVVDAVRLRERFVPAGTDGYRVVHAE
                     GDGLPGLVVDRYADVLVVQATTEGTDSARPLWLPPLRDLFPKASILQRNDLASRHGEG
                     LPLEDELLAGERPPGSVPFREHGLTFRADLAGGQKTGFFLDQRENRNLVRGLSAGRSV
                     LNLFSYSGAFGVAALAGGAARVTHVDVSEPALALARENHEANGQEPDRVETVAADVFE
                     DLRRRVGRGETWDVVVTDPPAFAKKTADVDRACRGYKDVNRLALKLLAPGGLLLACSC
                     SGPVSADLFQKVLFAASLDAGVPARIVERRGAGPDHPVSIDCPETEYLKAFLLSR"
     gene            15017..16168
                     /locus_tag="PLP50_04800"
     CDS             15017..16168
                     /locus_tag="PLP50_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA50903.1"
                     /translation="MNGPAAGVVLLGPIPPWRSGIADQTVRLARAMTRLGAPPLVITF
                     SRMYPRALYPGAADRGEGAFPTDVEVVPLLDGFDPLSFRRAAELAASRGARLVAAAFW
                     TSVFAPHLALFLSSHRAEAPDAVRLLLCHNLADHEAGPLRRALARTALRRSDVLAVQN
                     AGDLATARREVSEARAVLVPHPAEPAPRVPRAEARRLLGLPAESPLFLFSGLLRLYKG
                     WDVLLEAFSAVRREIPDALLVLAGEPWGEAKALAERPAPDGVLLRLRFLPPDERGLLF
                     DACDAVVCPYRHATGSGIAADALSHGRPVIGSDVPGLSVVVRDGTSGLLVSPGDPGLL
                     AGAMLRFVREGLGVRLSSGAEEVGALFSPEGHARTILALGGIGSGPAGC"
     gene            16215..17204
                     /locus_tag="PLP50_04805"
     CDS             16215..17204
                     /locus_tag="PLP50_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50904.1"
                     /translation="MAITKMFRDRVVSMEQTWDLGEKYLSLLSELVHEALPRFRTVGN
                     DFDHDRDFYFLLEQASEPDEERKVCFTRMLLADRDCIPLIADEPHAPVRERIVAAIQA
                     QAENAEIVVAYRAVLSEEEKAEAEEIDAAWRIEEAAREAARKSEEERRRAEKKRQREA
                     EDARRRAHREKQRDRGGEAAAATPQRGGEGGEGRSRKRRRGRGGSGRAEGASQAGGTA
                     AAGAPADAGAARPSGERPGGDRRGERPRGERPRGERPRPQKGAGAPSRPAEPQAPGAA
                     PQAPRPVPAGGAPNGGAPREGGGNRRRRRRGRGGSRPQGGGPESGAPQGGGGE"
     gene            complement(17222..18226)
                     /locus_tag="PLP50_04810"
     CDS             complement(17222..18226)
                     /locus_tag="PLP50_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA50905.1"
                     /translation="MEYRTLGRSGLRVSPLCLGTMMLGSWGNADEGECVKIVHRALDA
                     GINFIDTSNNYGEGTSEEILGKALAGRRNEAVLATKVWSRMGPGPNEQGLSRKAIQEQ
                     VEGSLRRLGTDVLDLYQIHRPDRSTPWEETLSTLTDLVRQGKVRYVGCSTSHAADPGA
                     SPWSVRLEAWELVETLWISERNGFERWISLQPPYSILRRTMEVDHFPVTRRFGLGNLV
                     WSPLEGGWLTGKYRKGAPNPLDSPRAARWIGDVGNPKFARRIEVVEKLLALAEEKGTS
                     LARFALAWTLRNPDVTSTILGPRTLAQLDDAIAALDVTVTDEDAARVDALVAPGTSAL
                     "
     gene            18343..19245
                     /locus_tag="PLP50_04815"
     CDS             18343..19245
                     /locus_tag="PLP50_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50906.1"
                     /translation="MIKKGCAIAAVVLLGVFGAYLYLLWGKVEAVPAIVLAGFGSLGL
                     LMVVGQLKAVLFGSGDVGALKRAEQGLPLEDGTEEAVWGRIEPLGAALSAPFSGRPCV
                     AYEYDAKKPSSADDENATPSTLAGFALAPSVIRSSRGDVRLLGFSLLTEFPETVVDDP
                     EAPHRAVRHVASSQMEEMGLAKLGALISAMDDAMADDDGSVRKDMKTKDATVADLEAC
                     VLTEKIVAPGETVTAIGIWDASRGGLVPKHRGKSAVVRLLPGGGAAMVVHGQKRPWGL
                     LFFSLLWAGFAHVFIWLAWKGGFE"
     gene            19247..20161
                     /locus_tag="PLP50_04820"
     CDS             19247..20161
                     /locus_tag="PLP50_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPA50907.1"
                     /translation="MPELPDVELYVEHLTRRLARRTLERVRVASPFLLRTVSPPLSDA
                     EGRTIRGFRRLGKRVVFELEEGPFLVVHLMVAGRFHWKDLARAPVPKKVGLAAFDVPG
                     ATLLLTEASPKKRAALHVVASEEALAALSRGGLEPLAAGLPEFAAALRAESHTVKRAL
                     TDPRLFSGIGNAYSDEILHAARMSPFALTRTMGDEEVARLHAATTATLSSWLARLREE
                     AGERFPEKVTAFRPDFAVHGRYGKPCPACGDPVQRLVYADNEANYCATCQTGGRLLAD
                     RALSRLLKDDWPRSLEELEEAKTARRER"
     gene            complement(20220..21419)
                     /locus_tag="PLP50_04825"
     CDS             complement(20220..21419)
                     /locus_tag="PLP50_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50908.1"
                     /translation="MKTRTFISTLLATLVAAAPLAGGEVAVGRFAPGETVAPFAAASP
                     EADSRTFVKTALLDGGRAVVDLPATGTGTLLVWVLPAGEPDAALRAGDGAAGRELETR
                     LTTPAGRVLGPDADGGRADAVRRFRIEEFGAEELGLAVGARQEALRVLAPEPGLHRLE
                     LSAPGAAAVTVAVAELDSPIVLTTWAAPLSRQPGEPVTLYARLLDGGNPVSGVISARI
                     APQGGPAGEVVTLFDDGLHGDGEAGDGHFAVTLEGLDLPAGPVEIRVEAEGRDGHGSA
                     FARTGAAGFVSERGSARLLPGSVRSEWLGEGDLRVLRVTATAAVREGGDFRLDVLAAG
                     APLADGSRPGLAWAERTGDLRAGHEELVVDLPAALLGDGPVSLDVRLLGLSRPGVAGR
                     ATLDVAR"
     gene            complement(21430..22257)
                     /locus_tag="PLP50_04830"
     CDS             complement(21430..22257)
                     /locus_tag="PLP50_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50909.1"
                     /translation="MRTSVLGLALAALLLVPSPALAKKGVVFVHGKGGSDLANQSVAN
                     AYWGSDMIRATTKGYAVPYLVCHYDGTQYMWVAAGQVAGQITTWMNANAVDEIQVVTH
                     SFGGVVLRWILSNPTYDSRYPAILSRIQRVNALAPPNRGSEAANLAGTLSGSWLTGWL
                     VSLVGQNNDSTKNCTTTSMAYYNQYYLKGSAGRPALPKPYYNVAGTGLWNDFAHGEDY
                     GLATLSGVAGMPGEDDGMVSQYSAQGAGTNWFLTLANHHHNRRNDYRKLGTSLAGTY"
     gene            complement(22477..24906)
                     /locus_tag="PLP50_04835"
     CDS             complement(22477..24906)
                     /locus_tag="PLP50_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPA50910.1"
                     /translation="MKAKLALALGVLGAVGLAALLPRFETVQPRGLSITRPAAVRIAD
                     AEATRLGIPVGASFRVETWEQSLLVEQEIAGDPATRLALERDPVVGPRLGAYRVTYFR
                     TGLEKYPEFGYVLVGKDGTVLGARVRARSEEVGGRPTEDEVRAAADRFVASRSFPGAP
                     SFDFDGARPNVLRDRVDHVVRYRVPHAGPPETVSFFLNVHFVGNRLSGWELDEEYRDG
                     RAFRYELGGSIVTTLGRFVVVFGLLFVLLGIFLRKYHAGEVGVGTGAWLFGAQLLLLL
                     ALEVLVARAQSFNVGFGGTDAFQTALAQAAIAFLFLDLPLATLVFLSWSVGESYARER
                     WGERLASFDALLRRDAVNASVGGALLTGLLLAPVVAAATLLPCLPALVLGGARPVLGE
                     LSNLVLESEAGPATMTLAGLAFAIPTAVVGLLFTLSAFHRRRLLWVGLLAAAAVGSVL
                     VPGLAPVGPETTRFLLGFGAPLAAAGVFLAKDLLASAVSLFGATLLLGLLPLTRSLEG
                     PALQGAAAALLVPLLLLLALAIAGLATRRTVEYRYEDLAPHVKRIVERERVKAEIDAA
                     NRIQAALLPSSDPKLPGVVVASHYRAATEIGGDYFDFLPLPGGRIGLAFGDVAGHGLT
                     SGIVMAMAKSALLVQAGHDPSPVRVMEALNETVRKTAPKRMLMTFFFGVLDPATGDLR
                     YASAGHLDPYVVRAGGGAPEPLSAWGFPLGVKRRTPFVEHTTHLGPGDRLVLYSDGLI
                     EALDDDGEPFGFGRFEEVLAGAAREGADGMRQALLSAVRRFTRNRPPEDDQTLVVLSV
                     GPTPAGSEAAA"
     gene            24999..25913
                     /locus_tag="PLP50_04840"
     CDS             24999..25913
                     /locus_tag="PLP50_04840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPA50911.1"
                     /translation="MKVFVTQPVPEPSTSDLLSGFEVVVGRPDAWPEEGRRHLPDSDA
                     LVPTPRERVTEELLELAPRLKVVANCAVGTDNVDLEACRRRGIVVTNTPGVLTEATAD
                     LTWLLILAVARRFREGDALIRSGTWDGWKPMELMGASLGGKTLGVFGPGRIGTAVARR
                     AEAFGMRVVVCDAADGLGAFERLLAESDVLTIHAPLTPETRGRFGAAELGRMRRGAIL
                     VNTARGPIVDEEALAAALASGHLGGAGLDVYEEEPAVHPGLLPLPNVVLLPHLGSATR
                     ETRLAMARTACAEAARVLCGEEPRHRVA"
     gene            complement(25915..27561)
                     /locus_tag="PLP50_04845"
     CDS             complement(25915..27561)
                     /locus_tag="PLP50_04845"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50912.1"
                     /translation="MSAGAVSFANALRAAGRSLSPRSTAEAAGVLAVGLFSGAFVVGE
                     YAGLAHLLRLGRSAATQLPALVPEILLERLLLGAFAGAGVLLLLGALTTAVSTLFLSE
                     ELLLRMALPIPHARLLLRQVAATIALASGPAALVALPAVVLAARSGAEGAAALGAAFL
                     AVAVLAGAAGSALALAVVAVFPPRRARLLAAFLSATGLAVAIVGARGVRPERFLDPSA
                     ALDLLLRAGTTPLAEPGGNPVAIAARAAARALAGDAAGLLHALLLLGGAALLTVLLAQ
                     LLAPLHLRLLRRSREEADATPRRRVGRKAASAERELLRAEVRTLLRDAATPAQLGTLA
                     AVFVLDLLNLKLLPSGDAASRDLVHGLQSGLGLFLVSALALRFAFPAVSTDGRAALLL
                     KSLPLSAARHLVVRWTVRAAPSLAAALLLAGASAWALEARGFPLGAALAVAAVGGLAI
                     PALQLGLGALFPRYDAPNPVSVALGPGGLVAMGLSTLLSLAPVLVVSEELRGLAETLL
                     GARLDGRLLLAAWGATSAALATAAMLAAARRLPRVDVSSS"
     gene            complement(27558..28304)
                     /locus_tag="PLP50_04850"
     CDS             complement(27558..28304)
                     /locus_tag="PLP50_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA50913.1"
                     /translation="MAPAPPAFAFERATKLYGDLLALSELTLEVPPGVVFALVGPNGA
                     GKTTAIGLLTGLLAPTAGRATVFGIDVEEDPVRAKRRLGFVPDRPYLWPRWTPRETLR
                     FVGAVFGAKGAALEERIEAELEAFGLGAAADRTNETLSHGTRQKVALAQAFLHDPSLL
                     VLDEPMVGLDPLAQKGLAERLKERTAAGAAVLLTTHHLALAEEVADEAGVLVKGRLVA
                     RGTPLAVSARGATGRLSELFFDLAADGGEP"
     gene            28403..30457
                     /locus_tag="PLP50_04855"
     CDS             28403..30457
                     /locus_tag="PLP50_04855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPA50914.1"
                     /translation="MRSMSALAIPVLTLSFTAVAAGPAVSPAPAGPRPVDPASFDPSV
                     KPCEDFYQHAVGGWLKANPVPADKSRWGSFEELADRNRAVLKAILEETSAKADWPKGS
                     VRQKVGDFFASGMDEAAIEKAGTKPLEPWLAKVDALKSSADLPKLMAELHGRRLMAGY
                     GFYVGQDQKETTRYLAMLSQGGLGLPDRDYYLKDDPKSKELRDKYAAHVARMLELLGD
                     APEAAKTKAASVMALETKLATAARTRVELRDPERNYNKRTVAELVAAAPGYDWAAYFG
                     ALGAAGLKELNVRQPEFATAFAALAASEPLDAWKAYFRWHLVRASADVLPKRFEEEAF
                     AFYGRTLNGVPQQEERWKRVQAATDRALGEALGQIYVERAFSPKSKERMKVLVENLRA
                     SLKERIAALPWMGDETKKAAQKKLAAFGVKIGYPDVWRDYSALEISRASYFENVVAAN
                     AFEAKRNLGKLGKPIDRTEWGMTPPTVNAYYSPTMNEIVFPAGILQPPFFWADGDDAV
                     NYGGIGVVIGHEMTHGFDDSGSRYDADGNLKNWWTEEDRKAYETRTALVVKQFDGYRP
                     LADLGVNGKLTLGENVSDLGGLKVAYEALRKALGTDPNAAEKVDGFTPAQRFFLNYAS
                     VWRNNIRDEALRVRLNTDSHSPGRFRVLGPLSALPEFHAAFGCAAGSPMVMPEAERPS
                     IF"
     gene            complement(30550..31683)
                     /locus_tag="PLP50_04860"
     CDS             complement(30550..31683)
                     /locus_tag="PLP50_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50915.1"
                     /translation="MRHHTTLPILLLALTAVASPAAAQTLPSVSIATFPANPAPGDQI
                     VLKLSGEWPDGCVPETGRTKLTTSGTKLHVNFNYSGIPGPCTAAVTPWALDVPVGPLA
                     AGTWAVEVTRTRTFTAPEPIGSGSISVVPLAAATVYVPGFFAPGASVSMASTLAAYNN
                     TDKDVTIAFAGAWDGLGAAEPPADATVGPNGATVLDSHTLRAGQHVQMLAITAPRRVA
                     LRATLERLETVPEGLAKVPESLGRLELPVFGELFPAGMTAVAGDVSLSASECAEGATS
                     RRRVNLTLFNAGDAPATFRVSAVSATPGPGLLPAEKEYAVPAASIVQFNDLSLEDLPV
                     CSAGGAWFRITGDQPFLAYLSTVRPETLPGVLPYEIFPAKLDR"
     gene            31803..32384
                     /locus_tag="PLP50_04865"
     CDS             31803..32384
                     /locus_tag="PLP50_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA50916.1"
                     /translation="MDAPDAPTDADLVARILRGEEELFTLLVDRYKLRLFRFVSRYLG
                     DPEDARDVTQEVFLKLYSALDSFDPRYRFSTWAFRIAGNAAIDHLRRRRIRAIPLELP
                     ADDESDRRSIDPVDGKPDPLEELSRTRLREALDLAIERLPDDYRELISLRHHGELAYE
                     EIAELKGMPLGTVKNKLFRARQALRDLLPKGTV"
     gene            32404..32907
                     /locus_tag="PLP50_04870"
     CDS             32404..32907
                     /locus_tag="PLP50_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50917.1"
                     /translation="MHARTTDGNPTPAELDLDRRMEVFLDEMVSSSDRETLPADFVSR
                     VAAARPFAPWEVARGAFWRLPVAVGLGLLAGSLGLALTPLWSLGPATAVTVWAELLAV
                     AFGSPVATLVAALPLLAEGTERAAGAVSPGAVALLGGAAALVAGSLLGALARFRRPEL
                     PAARSRS"
     gene            32921..33814
                     /locus_tag="PLP50_04875"
     CDS             32921..33814
                     /locus_tag="PLP50_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPA50918.1"
                     /translation="MARRLVLLLLPVLLSSATGVAAEPVDVPNGEFRSGDVVALGREA
                     RVEGVLRGTLVTILGDVRISGRVEEDVVALGGNVHLEPGATVGGDLLALGGEVHAGGP
                     GPAPVGGRLLTVGALEAAFAAELKTSPLALRPVRGLFLAFRLALLLGWLIVGLVLLRL
                     VPRPLTGAAELVPGRLTAVAAIGAAAVLAALLVSALLLVVLPATAGLFAAGLLVVALA
                     LAKAFGIAAVFVALGRRLTRTAARGSLLFGDPAALAVGLALLGSVSLVPVVGPLAWSI
                     ASLLGIGTALAAAARPRAAAF"
     gene            33884..34762
                     /locus_tag="PLP50_04880"
     CDS             33884..34762
                     /locus_tag="PLP50_04880"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPA50919.1"
                     /translation="MAIERVGVIGAGQMGAGIAHVAALAGKTVVLADVDAALAEKGLA
                     VVRKNLGRQVEKGKVTAEAAEAALARIAVSGDLAALADADLVVEAIVENEEVKRGLFS
                     RLDALLQPGAILASNTSSISITRLAAATKRPESVIGMHFMNPVPVMQLVEVIRGIATS
                     DGTTEAVVAAAKEMGKVPVECRDFPGFVSNRVLMPMINEAVFAVFEGVATPEAVDTIM
                     KLGMNHPMGPLTLADFIGLDTCLAILNVLHRGLGDDKYRPCPLLVQMVDAGWVGRKAG
                     RGFYRYSGPGGEKVAG"
     gene            34759..36621
                     /locus_tag="PLP50_04885"
     CDS             34759..36621
                     /locus_tag="PLP50_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA50920.1"
                     /translation="MICQVCGASNDLDAELCRKCQNKLLVVSGSSESYDEGPSGEGIS
                     LDEHLLERVSVLEEIVKRSAETLKALLEGFHRQEKNGFVVQTGLLALKDLLERKGLLV
                     EQEFVDLWEERVDQHMTVLEKRERFLERKERILAEHKGLGRERLVDLLGEAELAFFAL
                     DPDKAMALLEEAWKLDRRNAELAFFLGETWFNDGDSGKAASALRHVLEQRPQHFDALV
                     YSGVLENDLGRPEKAVDALKRALALRPDAFLPAFALGAIHAGAGQLAKAEALLKRAVA
                     IEENPQALSLLGTIAYQRGRLADAIDALQRAVQLDPDDEEGLFQLGLCYLDRGWTQKA
                     TERFRAALELSPNRIELQEAKKLLGPAGAGALPGLAGTVGERGGRTAAAAARDPRKAL
                     GLYRRAIKADPENPAVQIAYALVCSSVGRTAEAEATTRRVLSQKPDEEIAAAAWSTLL
                     ETLRSEGKFREGTQVAGEMLQSVTSSSARSIAYFQRAAALADMGERLDEALEDAEKAL
                     RLSPRELRHFPLATKGWVHYKRKEFRRAVECLSRAAELGETPTALAHLGLAYLAVGDA
                     KAARSAFERAKRRGGRPSERHGGLEARMLEQIRRNLQLAERVASRPGRRKPGPG"
     gene            36840..39632
                     /locus_tag="PLP50_04890"
     CDS             36840..39632
                     /locus_tag="PLP50_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA50921.1"
                     /translation="MTRLTRNPFLRAAALLGAVLLPALTWAGPARAQTADATVEVDVV
                     DETGVALPGVSVELKRAETGFARTSVTSAAGAARFPAIPPAAGYVARVALQGFEAVEQ
                     PLTLRIGQTARLRVVLRAQAVTEAVTVVGEAPLVDVYKIDSSTNIVPEQIKELPVANR
                     EFEKLAFIAPGVQRERGEFRFVTGGPVIGSGGNASQSTILVDGVDYTDPALGLAKTRV
                     SQDAISEFRVVNSRFDAEVGGSAGGALTVLTKTGTNELKGSLFAFYRAEGLRAQGALE
                     QDSSVDFRRGQYGVTLGGPIVRDRTHFFLSGEYVDEVRPTLYRPQGAFTAQAADLEVP
                     MDQLLAFGSITHSLSDSQTLLVKADLERYRQDNFRVGGVQDASYGQELQRDNYNFTGG
                     HTWVVGAATTNELRAQIGHRKYFEPTNSDAVADWFTNGVTLKTGGNILGDLLGEGDQL
                     EIRDTLYLHLTGKSGTHDVKVGAGWQHVNDRSIIDTYASGLFLWAGDTKAFPIAYGYG
                     VGSSDVEITTNRLAAFVQDDWRPLSNLTVSVGLRYDLDTNGNNEGFTHPLVPEPRERD
                     TNNVQPRLGLSWDVKKDGRFVARAGWGLFVGRNLLVPAFTELQQNGVTGRKLYTRANG
                     LLYGLPAFALDPSNPMTTGLPLPIDITLLDQELVAPEASQFSAGLTSRLWKTGLYLDV
                     EGIWVKGRKELIVHDVNWNGNANPTRPNKAYNQVNVYSNDGESEYKALTFALNGSLKG
                     GHLVTGSVTFSSKHNRSDDFSPEFPTGYPSDPADLPNEWGRGRSAERMRFVLSGVFRL
                     PWQLTVAPIFNYGSGQPWTRRYGYDFNGDGKTGDRMPGVDRFGMDGPVYRSFDLRLTK
                     AFELGSAGTLELVAEAFNLFDSTNYDVASIDGAEFLSGPTLANPAAAAVANPNYGKPS
                     ATLPSREFQLGLRFAF"
     gene            39751..40533
                     /gene="fabG"
                     /locus_tag="PLP50_04895"
     CDS             39751..40533
                     /gene="fabG"
                     /locus_tag="PLP50_04895"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPA50922.1"
                     /translation="MSIETGLAGRSVVVTGAAAGIGKATALRFAKEGAKVAAWDVADA
                     AALADELKAAGAADAFTAKVNVADAASVEAAVADVVARWGRIDVLVNNAGIVRDAQLV
                     KYKDGAVLATMTDEQWDAVIGVNLKGVFLCTRAVTPHMIAAGGGVVLNASSVVGLYGN
                     FGQTNYVATKAGVVGMTKTWARELGRFRIRVNTVCPGFVATEILKAMPQKVLDGMVAH
                     TPIGRMGEPDDIANAYVWLASDQATFVHGAVLSVDGGLVTGT"
     gene            40536..41525
                     /locus_tag="PLP50_04900"
     CDS             40536..41525
                     /locus_tag="PLP50_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPA50923.1"
                     /translation="MPRVARIVSTARYLPPIEVPNEVLKPRWPQFPDFVDKMEASSGI
                     RTRFYAPEDQATSDVALPAAKLALERAGLTPLDLDLIILGTDSPDYVTPATSVVLQGK
                     LGAKNAGTFDVGCACASFPTGIAVGSGLIAANPALKNVLVLGVYLMHKLADPNDPMVF
                     FYGDGAGAAVLQGGDEPGFLNSAMQAGGEYAQCWGIFAGGTAEPATVEAVQAGRTTVK
                     MVEKYPAEINHEGWPRLVRRLSAEGGFALSEIDQLIFTQVRMPSIELVMNDLGLPVEK
                     AHTIMEKWGYTGSACIPMALDDAIEQGKVKKGDLVVMIGSGVGYNQAGVAIRM"
     gene            41560..42504
                     /locus_tag="PLP50_04905"
     CDS             41560..42504
                     /locus_tag="PLP50_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA50924.1"
                     /translation="MNRPMKLLLAAAALLVLAAIVFILWARLRPLAAYAAAGRRALAK
                     AGLETARVPSPAGEQTYFRGGSGPVVVLLHGAGDQASAWSLVAPKLLPGRTLVVPDLA
                     GHGASAPEKGAISVPMLLSGLSAVLDAAAPGGKVTIVGNSLGAWLACLWAKEHPERVE
                     RLVLVNGGPLKHAAEGITLLPKDRAEARAAVEKLRDPGSIRVPVFVLDDVVRQAATGP
                     LARLAATSGAMEPHVLDGKLGGLDVPVDLLWGASDQLLPLSYAERMRVELPRARVTEL
                     PRCGHVPQQECPAAFAAALGKLLDGPPPERAAATPEVP"
     gene            42501..44042
                     /locus_tag="PLP50_04910"
     CDS             42501..44042
                     /locus_tag="PLP50_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA50925.1"
                     /translation="MIHADVLGERARLSPEKCALVFVPTGERFTYRELDARATRVAAF
                     LRDGLGLMKGDRVALLAHNRVEYVDLFFAAAKAGLVLVPLGTRMTPHELAFIVRNADV
                     SALVYGGEFAETVAELRAEAAVPRRIALDAPSAAGDLWLAAELAALPEAPFVRTRCGG
                     EDLHLLLYTSGTTGKPKGVMVPHRMTSWNAWNTALCWGLTEHDVSPVFTPLYHAGGLG
                     AFLLPVLAAGGTVVLHAGFDAADVWATVTRERCTVVLGVPTIWKLLLEHPSFERADLS
                     SIRWLISGGAPLPLYLIEAYQARGIVFKQGYGLTEVGVNCFSMSVEESVAKKGSIGRP
                     LMMTDAKLVDESGAEVTAGEVGELCLRGPHVSRGYWMNPAATAAALDDEGFFHTGDLA
                     RKDADGFFTIAGRRKDMLISGGVNVYPAEIEGELLLHPTVMDAAVVGVPDPTWGEVGV
                     AFVVLRPCESATKAELLAFAESRLARYKLPKDVVFVEALPRTAYGKVVKGELRDAYLA
                     GRGAA"
     gene            44039..44884
                     /locus_tag="PLP50_04915"
     CDS             44039..44884
                     /locus_tag="PLP50_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA50926.1"
                     /translation="MSSTALLAHQVDGEGEPLLLLNGGMMSFTAWDAYVPPLAAKHRV
                     IRCDLRGMLRSPGARPEGFPGHAEDLLALLDHLGVSSAHVAGTSYGAFAGLHLAALAP
                     DRVASLVAMTVADRVSAEMWAEAERMAVACREALAGGSREKVYDLVSAFAFSEEWAEA
                     HAEEIAARRAAVSFLPDSWFSGLASLLSALEGLDARPLLPRVTCPVLVLLAVGDRAMP
                     RPWGESLVAALPRGELAVVPGCGHGLVVEKPRETLAHLLAFLARHPLPVEAPTRQPER
                     NGGTP"
     gene            44881..>45652
                     /locus_tag="PLP50_04920"
     CDS             44881..>45652
                     /locus_tag="PLP50_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPA50927.1"
                     /translation="MKPIYIAAYQQSKFGKLLGATVPEIIRTAAEETCRSVKAEASAV
                     DVATIGAVCNLTLNEQGLLSGLLAMTPGLDGKPIESVENACATGGQAILAVAQKLMLG
                     DGDVGLAVGFEKMRDADGKMDGKLVGKVLGLYSHPDERPGKTFVFPHLFAEVMKAYMD
                     EWGATEEDLASVAATEYANARHNPYAQMQKNEVTLETVMRIEGFNRYVVEGLPLKTFD
                     SSQISDGWAGMLLATEEGLARLGVAKADCVRLAGWGQAT"
     assembly_gap    45653..45752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <45753..46241
                     /locus_tag="PLP50_04925"
     CDS             <45753..46241
                     /locus_tag="PLP50_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50928.1"
                     /translation="LGVAKADCVRLAGWGQATDPLKKEGRDVLRPRAANAAMAAAYRR
                     SGLSPADVGVAEVHDCFSVMGAIGAEVIGKAEPGKGARYWRDGKARVDGECGINTSGG
                     LIAKGHPIGATGIAMVGWCAWQLLGKVPKELQVANARAAATFNIGGPVCASVCTVLTP
                     AS"
     gene            46300..47157
                     /locus_tag="PLP50_04930"
     CDS             46300..47157
                     /locus_tag="PLP50_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA50929.1"
                     /translation="MGEIVPDREGFLEVGGHRIRWEFVGKGDKETVCLLNGLAMHTRA
                     WYGVLPRLLDAYDVVLYDYLGQGQSSAPDEPYSIPEFAGYLPRVLDALGIEKAHAVGV
                     SYGGFVAAEAARLFPERLHTLTLSGILLSHEELFSMYQEISLRFYRGGPEVFELYTHY
                     MYEKIFGEAFVSAMKESLPALRGRFHDRYRSQTHALIRLTRAQDPFFAALDGNLAGYA
                     AFPEPVLVVPGAEDRTIPPWVQRKLLSVFRDARWEPIEGSGHVVYLEKPDAFFGVLRR
                     FLSAKSTSY"
     gene            complement(47185..48738)
                     /locus_tag="PLP50_04935"
     CDS             complement(47185..48738)
                     /locus_tag="PLP50_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPA50930.1"
                     /translation="MSRRLLVPLLLALLARPVAAQAPAGLADPDPGARERAAASLAAG
                     GAAAVEPLVAALADADPFVAGAAADALARIGAASVPALVRALGDAREEVCVGAAVALG
                     KLGPRATEAVPALAQALSNPKAVVRWTAASALGAAGRGASPALPALRDALWDRDEDVR
                     RGATLALERIDPAAWLRAPSWEATVAVVERLVPILMREHHVPAVSVALVKDRTVAFSK
                     AWGVADAKTGAPATTTTLFEVASMTKPAFAYVALKLVEDGKLDLDRPLAEVVDLPAVP
                     GQPELTRITPRMVLSHTSGLPNWRPGGGERDGPLPVLFPPGSRFGYSGEAFFLLQRAV
                     EKVTGAPLEAYAKDALFAPLGMERASFAWAPELDAALATGHDEDGKPKARARYRHANA
                     AYTLVTTAPDYARVLTALLDPEAFGPKALSRAGVDAMLRRAVRADARDPIERPGRARG
                     GAVFWGLGWGINETPGGDVIYHSGANQTGFRCYGQLSPSRGTGIVVLTNGLGGGALWT
                     RLVAAIGDL"
     gene            complement(48735..>49442)
                     /locus_tag="PLP50_04940"
     CDS             complement(48735..>49442)
                     /locus_tag="PLP50_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPA50931.1"
                     /translation="RKGNLLLGALYGAETRYLVDDDANDSLAAAMESWAAELRAAGRR
                     PFQVPLGGSTPHGALGYVAAMRELAAQLGPGKVQLVAAVGSCGTLAGLALGARLFLPA
                     ARVIGISVSRAAGPIAARAAELAAGSARLLGLEPVLGPSEVEAHDALRGEYGVATETG
                     REAILESARLEGLLLDPVYTGKAMAGLFGLARAGLLDPAVPVVFLHTGGLPILFSFDD
                     AFDDAAPFARVLPGGAR"
BASE COUNT         6855 a        17668 c        18388 g         6431 t
ORIGIN      
        1 gtggccgttc ccggcccgta cgcggccttc gtcgcgcacc gcgccctcga cgcggctcgg
       61 agcgtcttcc tcttctccga caacgtccct ctcgccgacg aggtggcgct caagcggcgt
      121 gccgccgccc tcggcctcct cgtcatgggg cccgactgcg ggacggcgat cctcgccggg
      181 gtcgggctcg gcttcgcgaa ccgtgtcgag cgcgggccgg tcgggatcgt cggcgcctcg
      241 gggaccggga tccaggagct ctgctccctc ctcgatcgga gcggcgtcgg cgtctcgcac
      301 gccatcggca ccggcagccg agacctctcc gccggggtcg gcggcttgat gacggaggcc
      361 gggctcggtc tcctcgcgaa cgacccggcc acccgcgccc tcctcctcgt cgccaagcac
      421 ccggacgcgg gcgtcgcgga gcgcctccac ggtctcctcg cgacgctcgg caagccggtc
      481 gccgtccgct acctcggaga ggggcggcgc ggcgaggtgg acggcgtcct ctacgcgggc
      541 tcgctcgacg aggcggccga ggcagcggcc tcgcgcgtcg cgtcgacgcc ggccgcccgg
      601 ggcgacctcg cgttcgccgc gcgcgaggcg gtcgcgccgg taggagcggg cgggagccgg
      661 ctcgtcggcc tcttcggcgg cggctccctc gcggccgagg cgcggtgcgt cctcgaggcg
      721 cgggggatcg cgacgcgcgt gccggaggag aagctcgcgg cgaacgcgcc gctcccgccc
      781 gggaacctcg tcgtcgacac gggggacgac gcctacacgg tcggccggcc gcacccgatg
      841 gtggaccaga ccgttcgctg cgagctgatc cgcgccgcgg ggtcggaccc ctccgtcggc
      901 gtcctgctcc tcgacctcgt cctcggcgac ggcgcgcacc ccgacccggc gcccgagatc
      961 gtcgaagccg tcgccgcggc gcgcgccgcg cggggctccc ggcctctctc cgtcgtcgcc
     1021 tccgtctgcg gcacgcgccg cgacccgcag gggacggcgc ggcaggagga gacgctccgg
     1081 cgggcgggag tcgaggtcga gccttccgcc gcccgggcgg cggcgacggc ggcggacctc
     1141 gtcctctccg tccggcagga ggtggcccga tgagcgcgac gtcccttctc ggcggcccga
     1201 tcgaggtcgt ccacgccggc atcgatgccg tcgccgaggc accccgcgcc gccggcgcgc
     1261 ccgtcacggt cgtcgacttc cggcctcccg cggggggcga cacgcgccgg atggcgctcc
     1321 tgtcggagct cctctccggc cccgccgcgc gcgagatcga cgccgcgaac gcccgcgccc
     1381 tcgcccggct cctcgcgagt cgcccgcacc tcgtcgcgat cggcgtcgcg cgcgacgtcg
     1441 tcccggggat ggcggacgac ctcttcctcc acgccggccc tcccgtgacg tgggagcgga
     1501 tgtgcggccc gatgcgcggc gccgtgatcg gcggcctcct ctacgaaggg cgcgcgaagg
     1561 acgcggacga ggcggagcgt ctcgcggcga gcggcgtcgt ccgcttcgac ccgtgccacc
     1621 accacgacgc cgtcggcccg atggccggcc tcgtgacgcc gtcgatgccc gtcttcgtca
     1681 tcgaggaccg cggtggcgag cggcccggga cacggacctt ctgcacgctc aacgaggggc
     1741 tcgggaaggt cctccgctac ggcgccttct cggaggaggt cgtcgcgcgg ctcgtcttca
     1801 tgcgcgacga gctcgggccg gtcctcgccg aggcgctcgc gctccgcgga ccgatcgacc
     1861 tgaagcacct gatggcccag gcgctccaga tgggggacga ggggcacaac cggaaccggg
     1921 cggggacgtc gctcctcttc cgcgagctcg cgccggccgt cgtccgcgcc acggcggatc
     1981 gcgagcgggc cgcgcgggtc ctcgacttcg tgaacgggaa cgaccacttc ttcctgaacc
     2041 tctcgatgcc gatggggaag tgcctgctga aggcggcgga aggggagccg ctctcctcgc
     2101 tcgtctccgt catggcgcgg aacgggaccg acttcggcat ccagctcgcc tcgatgccgg
     2161 ggcggtggtt caccgggccg gcgctgaacg tgcgcggcct ctacttcccc ggccggacgg
     2221 aagccgacgc gaaccccgac atcggcgaca gcgcgatcac cgagacggcc ggcttcggtg
     2281 gcttcgcgat ggccgcggcc cccgcgatcg tccagttcgt cggcgggagc gccgaggacg
     2341 ccctggcggc gacccgcgcg atgcggcgga tcacgctcgg gccgaacccc ggcttcgcga
     2401 tcccctcgct cggcttctcc ggctcgccga ccggcatcga ccttcggctc gccctggaac
     2461 ggaacctcct cccgcagatc aacaccggca tcgcccacag ggagcccggc gtcgggcagg
     2521 tgggggccgg cctcgtcacc cccccgtggg aggcgttcca cgccgccctc gaggcgttcg
     2581 ccgaacacgt ccgcgcctcc cgcgcataga cgaaggagcc caccatgcgt ccgatcgagc
     2641 tctcgatccc cctcggcatc ggcaccccgg cctggcccac gtacgagccg ctccagatga
     2701 agtacttcaa gcggctcgcc ccgaacggcg cgaacggcca gctcctgacg cactcgaatc
     2761 acctcgggac gcacatggac ggcgagatcc acttctactc gcccggcaag gacatggcgg
     2821 ccctcgagct cgacttcctg atgcacgagg gggcgatcgt cgacctctcc gacgccgtgg
     2881 gcgactacga cgtctacacg tcgaagatgg tcgaggagcg ggtcgaggtg aaggacggcg
     2941 acatcctgat catccacacc ggctaccacc actacggctg ggaccagccc gaggcggacg
     3001 agatccgcta catggtcaag caccccgggc ccgaccgcga gttcgcggag tgggcgcggc
     3061 ggaagaagct ccgctggatc ggcgtcgact gcgggagcgc ggaccacccg atgaacacga
     3121 tcatccggac ctggatgccg cggcaggcga aggaggcgga cgcccacttc cgcgcgaagt
     3181 acgggatgcc gctcgaccag ttctacgacg acggcaagta ccagctcatg cacctcgagc
     3241 tcttcaacca cgggatcatc cacgccgagt gcatgggggg cgacatcgac ctcctcctga
     3301 accagcgcgc cgtcatcggc gccttcccgt ggcgcctcgt cgacggcgag tcgtgcatct
     3361 gccgtatcct cgccttcgtc gacgacgacc gccacgcggc gctcatggcg aagaaggaga
     3421 aggccgcgaa gacgaagtgg aacgacgtcg ccggcgctcc gaacgcctgg ctccacgagg
     3481 aggcgcggaa gaaggcctga ggccgacgcc cgcgacgcca cggagatgac ggacctcgcg
     3541 ttccgccccg ccacggccct cctcggtgcg ctggagagga aggagacgtc gagcgaggag
     3601 ctcctccgcc tctacctgac tcgaatcgag cgcctcggct cgttccatgc cgtcgtcacc
     3661 ctcgacaccg accgggcgct cgccgaggcc cgggcgtgcg acgcggagcg cgcgcgaggc
     3721 gagcggcgcg gaccgctcca cggcctcccg gtcacggtga aggactcttt cgagacggcg
     3781 gacctccgga cgacgtccgg cgggacgccc gagctgatgg cgcacgtgcc cgcgcgcgac
     3841 gcgacggtcg tggctcggct gaaggccgcc ggggcgatcg tcttcgggaa gacgaacctc
     3901 tcgacccagg cgatggacat ccagacgtac aacgccgcgt tcgggacgac gccgaacccg
     3961 tgggatgcct ctcggacggc gggcggctcc tcgggcggct cggcggtggc gatgtccacg
     4021 ggcctctccg cgctcgaggt cgggagcgac ctcggcggct cgatccggat cccctcggcg
     4081 ttctgcggcg tctttggcca ccggccgagc tacgggatcg tcccgacgcg cgggcacatc
     4141 ccgggacctc ccggcacgct cgccgcgccc gacctcgaga ccgccggccc gatcgcgcgc
     4201 tcggcggacg acctcgacct cgcgctccgc ctcctcgccg gcccggacga aggccacgcc
     4261 gtcgcctggc gcctcgagct cccgccgccg cggcggcgcg gcctctcgga gtaccggctc
     4321 gccgcctggc tggacgaccc ggcggcgccc gtcgacacgg cgctccggga gcggctcgag
     4381 gccgtcatcg gggagttgcg cgcggcggga gcggaggtcg acacggaggc gcgtcccggc
     4441 ttcgccttcg ccgacaacgc gcgcctcttc atgcaggtcc tctacgggag catgtcggcc
     4501 ggcttgccgg aggggcagtt cgagaagatc cgcgcccgcg ccgccgcgct cgccccggaa
     4561 gacgactcct tccgcgcgcg cctcgcgcgc gacacggcgc agacccaccg cgagttcgac
     4621 ctcgcgcgcg aggagcgcga gcggcggcgc gcccgctggg cggagctctt cgaacggtac
     4681 gacgcgctcc tctgcccggt cttcccgacg cccgccttcc cgcacgacca gagccccgac
     4741 ttcgcccgcc ggacgctcgc cgtcaacggc gcgcacgtgc cctacctcgg gacgctcctc
     4801 ggctggccgg cgctctccgg cctcgcctcg ctcccgggga cgtcggcccc cgtcggtttc
     4861 acgcgcgagg gcctgccggc cgggatccag gtcgtcgggc cgtacctcga ggaccggacc
     4921 gcgatccacg tcgcgaagct cgtcgcggag gtggcggggg gctgcgtcgt cccgccgggc
     4981 gtggagtagg cgcggcgccg ggagacgccg cgtccggggt cacggcgcag cgacgtactc
     5041 gacgaagaac tgctccgtca ggaagtcgtg gaccttcctc gggacgaggc cggcctcggc
     5101 catcgaagcg tcgacctcct cccgcgacat ccactacgac gggggcggtc cccagggtcg
     5161 ctcctccacg ctcttcggga cgaagtcgac gacgacgacc cgcccgccgg gcgcgaggcc
     5221 ggcacggacc tttcggagcc acgcggcgcg gtccttcacg tagtggagcg tgtcgacgat
     5281 gaggaccgtg tcgaggcccc cggccggcag ctacgggtcg tccttctgca cgaggagcgt
     5341 ccgtgcgttc gtcaggcccg cctgcttcag ccgtccgtcg aggtgggcga ggaggtcggc
     5401 gttcgcgtcg acggcgagga cgctccccga cgggccgacg gcgcgcgcga gccgaagcgt
     5461 gaagtagccc gatccggccc cgacgtcggc gatccgttcg ccgggcttca gggcgagagc
     5521 gcgcatcacc tcgtcgggct tctggtcggc gtcccgctcc ttccgctcga ggagccagag
     5581 gtagcccttc agccagaccg ccttcgcgag cgcctggaag cgggcgtcgt ccacgagcgc
     5641 gtcgaacgcc ccgtccttcc ggagggcgga ggcgtcgaag aggcctgctc cgtgggcccg
     5701 ctcgagggcg tcgagagcct ccgtccgctg cccgaggaag gcgcggaccc gggcgacccg
     5761 gtagagggct tcggcaaagg cctggccggc gccctcggcg gcctcctcgg cgagcgggag
     5821 ggcgcggctc cactccttcg cggcgagagc ggattcgagg ggcgtcggcg gggcgggcgc
     5881 cgcgggcgag gtcccgtcgg cgcgggccgc ggcagacgag gcgaggagcg cgaggacgag
     5941 cgccgcaaca gggcgtgacg gtcgggacgg acgcagacgt acctccttcg ccgccatcgc
     6001 ccgacgatac gggcctccac ggcccgggtc tcggggaatc cagacccgcc gaccgagctc
     6061 ggggagcggc tccgctccgg cgccgtcccc ccccggcgct acggcgcgag ccgcagtccc
     6121 gcccccggcc cgaccggaag gtcgagcgcg atccagccca tcaggatgag gctccaggag
     6181 acgaggaaga cgagcgtgta gggaagcatc gtcgcgatga gcgtcccgag gcccgccttc
     6241 gcgtcgtagc gctggaccgt cgcgaggatg agggcgaagt aggacatcgt cggcgagatg
     6301 acgttcgtga cgctgtcgcc gacgcggtaa gccgcctgcg tcagctcggg cgtgtagccg
     6361 aggagcatca gcatcgggac gaagacgggc gccatgaccg cccacttcgc cgaggcgctc
     6421 cccatcagga ggttcacgcc ggccgagagg acgacgaagc cgagcatgag cgggatgccg
     6481 ccgaggccgg tcgccttcag gagctcggcc cccttcacgg cgccgatgag gccgaggtgc
     6541 gaccagttga accaggcgac gaactgcgcg gcgaagaaga cgaggaccat gtaggagccg
     6601 agcgtttcca tcgacttcga catcgccctc acgacggcgg cgtcgttctt catcgtgccg
     6661 gcgccggctc cgtaggcgat gcccgagagc gccccgccga ggaagatgaa cgggacgatc
     6721 gccgtcagga gcggcgagtg gaggacgccg ccggtcttca ggtcgcgcag gaacccgcct
     6781 tcgggaacga cgccccagag gacgacggcg gcgaagagcg cgaggacgac gacagcgttg
     6841 cggagcccct tcttctcggc ggcgtcgagg ggacggagct ccccgtcggc cttctcggtt
     6901 ccggcgtagg cgccgaggcg cgggacgacg agcttctcgg tgacccacgt cccggcggcg
     6961 gcgatgagga acgtggagac ggcgaggaag tagtagttcg cggcggggct gacgacgtag
     7021 gaggggtcga cgatgtgcgc cgcctcctgc gtgaggccgg cgaggagcgg gtcgatcgtc
     7081 ccgaggacga ggttcgcgct gtagccgccg gagacgccgg cgaacgcggc ggcgaggccg
     7141 gcgatcgggt gtcgtcccgc ggccaggaag aggacggccg cgagcgggat gaggaggacg
     7201 tagccgacct cgctcgccgc gttcgacatg actccggcga agacgacgac gaacgtcagg
     7261 agcttccgcg gggccgagag gacgaggagg cggaggagcg ttccgatgag gccgctcccc
     7321 tccgcgacgc cgattccgag gagcgcgacg aggacggtgc cgagcggcgc gaagctcgtg
     7381 aagttcgaga cggccttctc gaggacgagg tggaggccct cgaccgacag gaggttgacg
     7441 accgtgatcg cctttcccgt tcccgggtgg acggcgctga gccccgccca ggagccgatc
     7501 gccgaggcgc cgaggacggc gagcgcgagg aggaggaaga tcgtcgcggg gtgggggagc
     7561 gcgttcccgg ctctctccac gatcccgagc atccgggaga agaggtcccc ccggacggcg
     7621 tccggcttcg ccgcggcgcg gccccgctcg ttcttcggcc ccactcgtca cctctccgcc
     7681 cggcgtgccg cctgacgcga tgggatctca acgccccggg gggggcgggt attcccgggg
     7741 ccgcgagaaa tcgtcggggg gcgaggtgcg acggggccgg ccggcctctc agagagagac
     7801 cttccggaag aggagcgtcg cgtcgccgcc gttcgaaggg gcgaccgtcg cggggcggga
     7861 gcggccgatc gtcgtcgcgg acggcggaag ccccggcgtc tcgtcgagga cgaacgcttc
     7921 gcgtggcgtg gccgggagcg tcagctccag gacgaagccc tccggcggcg cgccacggac
     7981 ggagagccgg agcggccttc cgttcgtggg cgtcctccgc aggtagggag gcggcagagg
     8041 atgcccctcc acccggatct cctcgacgcc ggcggcgtcg gggaagacga gcccgaggcg
     8101 cggcgccctg cgcgcggaag cgaggcggag gcggacgcgc cgccgcccgt ccgggagagg
     8161 cgcggaatcg acgatcgaga gctcgggaaa cgcgagcgac gtggacggga ggggcgccgc
     8221 gaacggagag gccttgctcg cccaagggta gaggccgggc gccttctcga agcggccggc
     8281 ggccgcgacg gcagggtgga ggcgcccgga ggcggggctg taggcgaggc gcgcttccgg
     8341 gcgcccctcc tcctcgacgg cgatcatcgt gccgtgctcg ggttcgtcgg gcgtcggttt
     8401 cgagagagcg agggccgcgg cggaagcggc gacgaggagg gcggcgagga cgccgaccgg
     8461 ccggcgccag gcgccacgcg tgctgtccac cggcagcgtc gcgaggacga tcccgaacgc
     8521 cgcggcgacg accggcaggg cggccggtcc gagcgcgtcg gggaggaaga gcgcgagcgg
     8581 gaggaggacg aggccggagg ccgcgaacgg caaccagggg gacaccggct ccggaagcgc
     8641 gagccgcgcg ccgccggcga cgagagcggg ggcgacgagg aggtaggagg tctcgggcca
     8701 cgcgagcgcg aggacgagcc cgaggagcgc ggtcgaggtc caggcccgct cccatgacgc
     8761 ggcggcatcg cccgcgcggc gcgcctcggc ggagaggacg ggaagagccg cgaggagccc
     8821 cgccgcgacg gcggccgcga gggatgccca cggcctcggg acgaacgtga cggggaaggc
     8881 gccgagcgcc cggaggagct gccaggcgag gacgccggag agggccgcgg cgaaaggagc
     8941 gaggaacggg acgagagcgc ggatacgcgg caggaagcgt ccgccgcggc ggaggcacgc
     9001 gaggcggacg aggagaagcg cgaggagcgc gacggccggg gccgcgggca acggccaggc
     9061 gaggacgaac gcccccgcca cggagaagaa gacggcgcgt ccggagggcg gttcgagcag
     9121 gtcggtctcc gccagcgcgc gggcgagcgc gagaacgttc tccccctggt gctggaggga
     9181 ggcccgcgag aggttcgcga gggagtcctc gcgcgtgtgg tagcgcgccg cggagccgat
     9241 gaacgcgagg ttcaggccgg cgagcccctc ccgcttgtag accgtcaggt cggtgtcgtt
     9301 cgggagccgg tcgtagacga acgcgaagag cgagctcgtg acggggcgcg ggaggcggga
     9361 gacggcgcgc cggacgagcc acgcgttgtc ggcgctcgtc tcgaacagga ggctcgggcc
     9421 ggtcgtcccg cgcgcttcga ggttcacgac ggcgccgacg gagcgcgcgc ggggggagcg
     9481 tgcgaacagc tcggcgccga ggagcccgtc ttcctcgccg tcgtcgaaga ggaggaggac
     9541 gggattccgc cgctgggcgg cgaggagggc ccgggccgcc tcgaggagga cggcgacgcc
     9601 cgagccgtcg tcgctcgctc ccgggcccgc gccgacggag tcggtgtgcg ccgtgaggag
     9661 gaccgcgggg cctcccgtcg ttccgggcag ctcggccagg acgttcacga gccgcgcaca
     9721 ggagccgtgg ggtgcgcagg cgaggccctc gtcgacgttc ggcgtcagcc cgagccgccg
     9781 gacctcgtcg agaaggcgtt cccggaacgc cgcgtgcgcc gggctgccgg gcgtccgcgg
     9841 cgccccgtct ccgaggaggc gcccgaggac ctccgccgcc ctcgccgcgg agaactccgt
     9901 cgccgcggcg tccgcctcgc gcggggaggg gggcgaggag cgccaggcgc agaccgccgc
     9961 gagggcgaga agggcgagga gggccgtggg gaggcggacg gcacgcatcg aacgctgcgg
    10021 cgaggcgtga aggctcagct ccgcttcacg cgagcgagga gctccttcac ggcgtccttg
    10081 tggacgagga acgcgagcat cttcagcttc acgtagtcct cgtggaagaa gtagtagccg
    10141 gggtgggcgt tgttccagga gctcgaccag gagtccttca cgaggaacca gtccttcccc
    10201 ttccgcgccg tgatgccgac gacgtggagc ccgtggtcgt ccgtcgtgct cccgttcgcg
    10261 aagcggaacg cgcgggccgc ctcgtcgatg aagggggagg ggacgtccca cgacgggacg
    10321 acggcgattc ccggggggcc gatggagtag ccgggctcgg acatgtcgcc ggcgacgacg
    10381 agcgaatacc cgcccctcac ggcgcccttg atcgccgcga ggaacgcgtc gagcgggacg
    10441 ttgacgtact ccttcccgtg ccaccagttg tcggggacgg cgtactcggc cttctcccag
    10501 tacggctgct ccatcgtgga gaggaacgcg acgtagtcgt cgggatcgag cctcacgacc
    10561 ttcgcgaggt actccttcgg tgtcatcgcc gccccgtcga cgacgacggt ctcgggcgga
    10621 gcgccgaggt ggtggtcgag gatcgcccgg acggtcgaga cgacggcggc ctcgttccac
    10681 gctccggagg ccttcacgcc gtcgaggtac gccttgatct ccgcgtagag cgtcgcctgg
    10741 tggtcgtgct ccctcttccc gtccctcaga cccgtgtagg actccgcggg aagggcgccc
    10801 tggttcctca gcgcgcgcag gacggccgcc ggctgcgagc cctccccgta gaccgacgtc
    10861 ccgcgcgagc ggacccaccc cttcgccttc tcgacgtgct cccagtagac cgtgtggagg
    10921 atcgagagcc gaagctctct cttcgtgagc cggtggacct ccgactcgag gaacgaggtc
    10981 gtcgagaagc accagcacat ccccgagagg ccctggcaga ccggcggctt gtgccagacg
    11041 ctcgtgaact ccgagacggc cttcggcgcg tcgacggcgg agaaatcgac ccacatccgc
    11101 ttcttcggcg ccggggtctc cttcgccctc acctcggcgc ggatcgcgtc gagcttcgcg
    11161 tcgaccttct cggcgaagac gacgccgtcc cgccgctcct cggcggcggc cggggacgcg
    11221 aggaagacgg gcagggcggc gagagcgagg gcggtgcgtc tcatcgagga cctccgaacg
    11281 gcgcagtctg gcgcgatcga cctccggcag caaccaccga tcgaaaccgg tgccaggcgt
    11341 gaaatcgtgt attgatttcc ggaagccgaa ggctgccgcc gcgacggcag gcggtggaca
    11401 tccgcgtgac ggatcgggga tgctcccgag aagcccgaga tttcgctgca ggaggccccg
    11461 atggttcctc tgacgtcgtt gtggattccg atcctcgtct ccgcggtcct cgtcttcgtc
    11521 gtcagctccg tcatccacat gctcctcccg gtccacaaga acgacttccg gaagctctcg
    11581 cgggaggacg acgcgctcga cgcgatggca aagctcgggc tcgagcccgg ggactacatg
    11641 ttcccgctct gctcgagcat ggccgagatg aaggacccga ggttcctctc gaagatggag
    11701 aaggggccgg tcggcgtgct gacggtcatg ccgagcggcc cccccgcgat gggtccgagc
    11761 ctcgcgctct ggttcgtcta ctgcgccgtc gtcggtctct tcgcggccta cgtcgccggg
    11821 agggccctcc ccgtcggggc gcactacctc gcggtcttcc gtttcgcggg cgtcacggcg
    11881 ttcgtcggct actcgctcgc gctctggcag aacacgatct ggtacaagcg ctcggcggtc
    11941 tccaccctca agtcgacgat cgacggcctc gtctacgcgc tctgcacggc cggtgtcttc
    12001 ggctggctct ggccgaagta gccctcgggg tcgacgtctc cgtcggccgc cggatccgcg
    12061 ggcgggaacc cggcgtcagc ggccggaccg gggcgccggg gaggtcttct tcgcggccag
    12121 ccgcttccgc acctttccga gcgagacgtt gagcgggttg tcccggtgca ggaaggagat
    12181 caccggcggc cgccggctcc cgaactccgt ccggagacgc acgaggccgg gatgccgcgg
    12241 atcgagggcg agacccttct cgagcgcctc gacggccttg cgccggctcc ggcccgccat
    12301 ccagacgcgc gccatgttgt agtaggcgtc cgggttgaag tggtctttct cgacgccgag
    12361 ccagcagaac tcggcggcct ccttgatgcg tcccttgtgg agcgcgaggc agaggccgta
    12421 gctggagagg agcggacccg acaggcggtg cttctcgcgg ctcaggtggc ggtaggcctc
    12481 ggcgaggaag atgaggcctc tctcgtattc cccggcccgg gcggcagcga ccccgagctc
    12541 gacgagatcc tcgaggttcc ccgagcgcca ctcggcgagg acggtttcgg gtgtgagcgt
    12601 gtctgtcggc atgagcctcg gcagtctggg aacaggcgtc gggcgtgtca agcgccggtc
    12661 gtgacgcgtt ggacggcggc gcgcagggcc ccgccgtcga ggcggagcgc ctccacccgc
    12721 tcggctgcgg ctcggagggt ctcccgggcg gcatcggcct ccgcgcggag ccgggtcgcc
    12781 tcggcggccc gcgcctcggc ggcagcccgg acggtgtcga gcctctcgcg aacgaggcgg
    12841 gcggactcgg cggcgtcccg cgtcgaccgc actccctcct cgagcgtgcg accgagctgg
    12901 gagcagaggc cttccaggcc acccagacgc tccgaggccc gggcgccgcg ctcctgtccc
    12961 gccgtggcgg caccgacgga ctcctccacg agcgcggaga cccgtcgggt gcccccttcg
    13021 ttccggcgcg cggcctcgat cagctcgcgc gagagtcgcc gggcctcctc gaagaggctc
    13081 cgcagctccg cggccgcctc ctcgaacgga gcgccggctt cgccggcccg cgccgagagg
    13141 agggagacgt tcaggccgag gagattcgcc cggtcggcga ggccggagac cgaggacgcg
    13201 agcggcatca gggcctccga ggtccgggcg agctcggaga ggccgcggga aaggccggtg
    13261 tcgagctcgg cgacgcggtc gagagccgac cggaggctgc gaacgtcgtc tgccaggcgg
    13321 gacctctcgt ccgagatccg ctccgcggtg gcgagagctc gcccgagtcc gtcgacgtcc
    13381 acccgaggcg gggcgggatc gtcgccggcg aggaccggcg aggagggcgg gagcggcgac
    13441 cggagacgcg cggcggtgtc ggcgaggacc gggtcggcgg cgcgcatcga gtcgccgagg
    13501 cgctccgcgg ggccgagcag tgtggcggcg aagctctcga aggacggggc cctctcgtac
    13561 ggggcgggct ccggaggcgc aggcaagcgc ggggccgcgg cgggcggagc cggcggcgtc
    13621 gccgcgggag gtggctctgg gagcggcgtc gaagacgggg cggtggcgtc gggccgggca
    13681 acggcgagcc ggtcttcgag gcggtggacg cgtccccgct cggtcttcag cgcgctccag
    13741 gcggaggcca gcgcgacgac cgaggcggcg aggaggaggg cgagaagaag ctccatccgg
    13801 agggaaagcg tacaccggcc cggcgctccc gcctaaactc cgggtccggt ttgacgacga
    13861 cccttctcct ccgcgcgggc cgcgacgacg ccgcccgccg ccggcacccg tggatcttct
    13921 cgcgggcgat cgcgggcagc ccgcccgcgg gcgaggtcga ggtgcggtcg gcgtcgcggc
    13981 agctgctcgg ccgcgggttc gcgtcccccc gctcctccat cgccgcccgc atctggcggt
    14041 gcggggaggg gccgctcgac gcgccgtacg tccgcggcct cgtggtcgac gccgtccgcc
    14101 ttcgggagcg attcgtgccg gccgggaccg acggctaccg ggtcgttcac gcggaggggg
    14161 acggcctgcc gggcctcgtc gtcgaccggt acgcggacgt cctcgtcgtc caggcgacga
    14221 ccgaagggac ggattccgcg cggcccctct ggctccctcc gctccgcgat ctcttcccga
    14281 aggcttcgat tctccagcgg aacgacctcg cgagccgcca cggtgagggg ctccctctcg
    14341 aggacgagct cctcgccggg gagcggcctc ccgggagcgt cccgttccgc gagcacggcc
    14401 tcacgttccg cgccgacctc gcgggcggac agaagaccgg cttcttcctc gaccagcggg
    14461 agaaccggaa cctcgtccgc gggctttcgg cgggccggag cgtcctcaac ctcttctcct
    14521 actcgggcgc gttcggtgtg gcggcgctcg ccggaggcgc cgcgcgtgtg acccacgtcg
    14581 acgtctccga gccggccctc gccctcgcgc gggagaacca cgaggcgaac ggccaggagc
    14641 ccgaccgggt cgagaccgtc gcagccgacg tcttcgagga cctccgccgg cgcgtcgggc
    14701 gcggcgagac gtgggacgtc gtcgtgacgg accctcccgc cttcgcgaag aagacggcgg
    14761 acgtcgaccg ggcctgccgc ggctacaagg acgtgaaccg cctcgcgctg aagctcctcg
    14821 cgcccggcgg tctcctcctc gcgtgctcct gctcgggacc cgtctccgcc gacctcttcc
    14881 agaaggtcct gttcgccgct tcgctcgacg cgggcgtccc cgcgcgaatc gtggagcggc
    14941 gcggcgccgg cccggaccac ccggtctcga tcgactgccc cgagaccgag tacctcaagg
    15001 cgttcctcct ctcccgatga acggtccggc cgcgggcgtc gtccttctcg gtccgatccc
    15061 gccctggcgg agcggcatcg ccgaccagac ggtgaggctg gcgcgtgcga tgacgcggct
    15121 cggcgcgccc cctctcgtga tcacgttctc gcggatgtac ccgcgcgccc tctatcccgg
    15181 cgcagcggac cgcggagaag gggcgttccc cacggacgtc gaggtcgtcc ctctcctcga
    15241 cggcttcgac ccgctctcgt tccgccgggc ggccgagctc gcggcctcgc gcggagcccg
    15301 gctcgtggcg gcggcgttct ggacctcggt cttcgcgccg cacctcgccc tcttcctctc
    15361 ctctcatcgt gccgaggcgc ccgacgccgt ccgcctcctc ctctgccaca acctggccga
    15421 ccacgaggcc ggaccgcttc gccgggccct cgcgcggacg gcgctccgga ggtccgacgt
    15481 cctcgcggtg cagaacgccg gcgacctcgc gacggcgcgg cgcgaggtgt ccgaggcgcg
    15541 ggccgtcctc gtgcctcacc ccgcggagcc cgccccgcgc gtccctcgcg ccgaggcgcg
    15601 gcggctcctc ggcctcccgg cggaatcccc gctcttcctc ttcagcggcc tcctgcgcct
    15661 ctacaagggc tgggacgtcc tcctggaggc gttctccgcg gtccgtcggg agatccccga
    15721 cgcgctcctc gtcctcgcgg gagagccgtg gggagaggcg aaggctctcg cggagcggcc
    15781 cgcgccggac ggcgtcctcc tgcgtctccg cttcctcccg ccggacgagc gcggcctcct
    15841 cttcgacgcg tgcgacgcgg tcgtctgccc ctaccggcac gcgaccggct ccgggatcgc
    15901 cgccgacgcg ctttctcacg gccgaccggt gatcggctcg gacgttcccg gcctctcggt
    15961 cgtcgtccgg gacgggacct cgggcctgct cgtctctccc ggcgacccgg gccttctcgc
    16021 cggggcgatg ctccgcttcg tccgcgaggg cctcggagtg cgcctctcgt ccggggcgga
    16081 ggaggtcggt gcgctcttct cacccgaggg gcacgcccgg acgatcctcg cgctgggagg
    16141 aatcgggtcc gggccggctg gttgctagac tcggcgcgat cccgcgcgca ccgcgcggac
    16201 gaggaccacc gcgaatggcg attacgaaga tgttccggga ccgggtcgtc tcgatggagc
    16261 agacttggga cctcggagag aagtacctct cgctcctctc ggagctcgtc cacgaggcgc
    16321 tgccccgttt ccggaccgtc ggcaacgact tcgaccacga ccgcgacttc tacttcctcc
    16381 tcgagcaggc ctcggagccg gacgaggaga ggaaggtctg cttcacccgg atgctcctcg
    16441 cggaccggga ctgcatcccc ctgatcgcgg acgagccgca cgcccccgtt cgggagcgga
    16501 tcgtcgcggc gatccaggcg caagccgaga acgccgagat cgtcgtcgcg taccgcgccg
    16561 tcctctcgga ggaggagaag gcggaggccg aggagatcga cgcggcctgg cggatcgagg
    16621 aggcggcgcg cgaagccgcg cgcaagtccg aggaggaacg ccgccgcgcg gagaagaagc
    16681 gacagcggga ggcggaggac gcccggcgcc gggcccaccg cgagaagcag cgcgaccggg
    16741 gcggcgaggc cgccgctgca acgccgcagc ggggcggcga ggggggcgaa gggcggtcga
    16801 ggaaacgccg ccgcggccgg ggcggcagcg gccgcgcgga gggcgcgtcg caggccggag
    16861 ggacggccgc ggcgggcgca ccggccgatg ccggcgccgc tcgtccgtcc ggcgagcggc
    16921 cgggcgggga ccggcgcggc gaacggcctc gcggggaacg cccgcgcggc gagcgtccgc
    16981 ggccccagaa gggagccgga gcgccgtcgc ggccggcgga gccgcaggct cccggagcgg
    17041 ccccgcaggc tccgcgcccg gtccccgcgg gcggagcgcc gaacggcggc gcaccgcgcg
    17101 aaggcggcgg gaaccgcagg aggcgccggc gcggacgcgg cggcagccgg ccgcagggcg
    17161 gcgggccgga gagcggggcg ccgcagggcg gcggcggcga gtagggaccg cccgcggccc
    17221 gctagagcgc gctcgtcccg ggggcgacga gcgcgtcgac gcgggcggcg tcctcgtccg
    17281 tcaccgtcac gtcgagggcg gcgatcgcgt cgtccagctg ggcgagggtg cgcggcccga
    17341 ggatcgtgga cgtcacgtcc gggtttcgca gcgtccaggc gagcgcgaat cgggcgaggc
    17401 tcgtcccctt ctcctccgcg agcgcgagga gcttttcgac gacctcgatc cgccgcgcga
    17461 acttcggatt gccgacgtcc ccgatccatc tcgcggcgcg cggcgaatcg agcgggttcg
    17521 gcgcaccctt ccggtacttg cccgtgagcc agcccccctc gagcgggctc cagacgaggt
    17581 tcccgaggcc gaagcgccgt gtcacgggga agtggtcgac ctccatcgtc cgccggagga
    17641 tcgagtaggg cggctggagc gagatccagc gctcgaagcc gttccgctcg gagatccaaa
    17701 gcgtctcgac gagctcccag gcctcgaggc ggacgctcca cgggctcgcg ccggggtccg
    17761 cggcgtggct cgtcgagcag ccgacgtagc gcaccttccc ctggcggacg aggtccgtga
    17821 gcgtcgagag cgtctcctcc cacggcgtcg agcggtcggg gcggtggatc tggtagaggt
    17881 cgaggacgtc ggtccccagg cgccgcaagc tcccctcgac ctgctcctgg atcgccttcc
    17941 gcgagagccc ctgctcgttc ggtccgggac ccatgcggga ccagaccttc gtcgcgagga
    18001 cggcctcgtt ccgccgtccg gcgagagcct tcccgaggat ctcctccgaa gtcccctctc
    18061 cgtagttgtt cgaggtgtcg atgaagttga ttcccgcgtc gagcgcgcgg tggacgatct
    18121 tcacgcactc gccctcgtcg gcgtttcccc agctcccgag catcatcgtt ccgaggcaga
    18181 ggggcgagac cctgagcccc gagcgtccga gcgtccggta ttccatcggg acctcccgcg
    18241 gcggcggccg cgtgcggacg ttacgcccgg ccggtagact cggcgagacc gttcgaatgc
    18301 cgttcaccgg gggggcgccg gggccccctg cgaggaggga ccgtgatcaa gaagggctgc
    18361 gcgatcgccg ccgtcgtcct gctcggggtc ttcggagcct acctctatct cctctgggga
    18421 aaggtcgagg cggtccccgc gatcgtcctc gcgggcttcg gctcgctcgg gctcctgatg
    18481 gtcgtcggcc agctgaaggc cgtcctcttc ggctccggcg acgtgggcgc tctgaagcgg
    18541 gcggagcagg gcctcccgct tgaggacggg acggaggagg cggtgtgggg ccggatcgag
    18601 ccgctcggtg ccgcgctgtc ggcgccgttc agcgggaggc cctgcgtcgc ctacgagtac
    18661 gacgcgaaga agccctcctc cgccgacgac gagaacgcga cgccctcgac gctcgccggc
    18721 ttcgcgctgg ccccgtccgt catccgctcg agccgcggcg acgtccgcct gctcggcttc
    18781 agcctgctca ccgagttccc cgagacggtc gtcgacgacc cggaggcgcc gcaccgcgcc
    18841 gtccggcacg tcgcctcctc gcagatggag gagatggggc tcgcgaagct cggcgcgctg
    18901 atctcggcga tggacgacgc catggccgac gacgacggct cggtccggaa ggacatgaag
    18961 acgaaggacg cgaccgtcgc cgacctggag gcgtgcgtcc tcacggagaa gatcgtcgcg
    19021 ccgggcgaga cggtgaccgc gatcgggatc tgggacgcgt cgcgcggcgg cctcgtcccg
    19081 aagcaccgcg ggaaatcggc cgtcgtccgc ctccttccgg gcggcggcgc cgcgatggtc
    19141 gttcacgggc agaagcggcc ctgggggctc ctcttcttct ccctcctctg ggccggcttc
    19201 gcgcacgtct tcatctggct cgcctggaag ggcgggttcg agtgacgtgc ccgagctccc
    19261 cgacgtcgag ctctacgtcg agcacctgac gcgacgcctc gcccgccgga cgctcgagcg
    19321 ggtccgcgtc gcctcgccgt tcctcctccg gacggtctcg ccgcctcttt ccgacgccga
    19381 ggggaggacg atccggggct tccgaaggct cggaaagcgg gtcgtcttcg agctcgagga
    19441 ggggcccttc ctcgtcgtcc acctgatggt cgccggacgc ttccactgga aagacctcgc
    19501 ccgggcgccg gttccgaaga aagtcggcct cgccgcgttc gacgtcccgg gcgccacgct
    19561 cctcctgacg gaggcgagcc cgaagaagcg ggcggcgctc cacgtcgtgg cgtcggaaga
    19621 ggcgctcgcc gcgctctcgc ggggcggcct cgagccgctc gcggcgggcc ttccggagtt
    19681 cgcggcagcg cttcgcgcgg agagccacac ggtgaagcgg gcgctgaccg acccgcgcct
    19741 cttcagcggc atcggcaacg cctactccga cgagatcctc cacgcggcgc ggatgtcgcc
    19801 gttcgccctc acgcggacga tgggcgacga ggaggtcgcg cgcctgcacg ccgcgacgac
    19861 ggcgacgctc tcgtcgtggc tcgcgcggct ccgcgaggag gcgggggagc gcttcccgga
    19921 gaaggtgacc gccttccgac ccgacttcgc cgtccacggc cgatacggaa agccgtgccc
    19981 cgcctgcggg gacccggtcc agcggctcgt ctacgccgac aacgaggcga actactgcgc
    20041 gacctgtcag acggggggcc gtcttctcgc ggaccgcgcc ctctcccgcc tcctgaagga
    20101 cgactggccg cggagcctcg aggagctcga ggaggcgaag acggcccgcc gggagcgctg
    20161 aagacggacg ccccggccgc gagcggccgg ggcgtccgcg aagcgaagag agcggccgat
    20221 taccgggcga cgtcgagcgt cgcgcggcca gcgacgccgg ggcgtgagag gccgaggagg
    20281 cggacgtcga ggctcacggg gccgtcgccg agaagggcgg ccgggaggtc gacgacgagc
    20341 tcctcgtgtc cggcgcggag gtcgccggtg cgctccgccc aggcgaggcc ggggcgggag
    20401 ccgtcggcga gcggtgctcc ggcggcgagg acgtcgaggc ggaagtcgcc cccctcgcga
    20461 acggcggcgg tcgccgtgac gcggagaacg cggagatctc cttcgccgag ccactcggag
    20521 cggacggagc cgggaaggag gcgcgcgctc ccgcgctcgc tcacgaaacc ggcggcgccg
    20581 gtacgcgcga aggcgcttcc gtgcccgtcg cgcccctcgg cctcgacgcg gatctcgacg
    20641 gggcccgccg gcaggtcgag gccctcgagc gtcaccgcga agtgcccgtc gcccgcctcg
    20701 ccatcgccgt ggagaccgtc gtcgaagagc gtcacgactt cgccagccgg tccgccctgc
    20761 ggcgcgatcc gggccgagat gacgcccgag acggggtttc cgccgtcgag gaggcgggcg
    20821 tacagggtga ccggctcgcc cggctgccgg gaaagcgggg cagcccacgt cgtcaggacg
    20881 atcgggctgt cgagctcggc cacggccacc gtgacggccg cggcgccggg ggcggagagc
    20941 tcgagccggt ggaggccggg ctccggcgcg aggacgcgga gcgcctcctg ccgcgcgccg
    21001 acggcgaggc cgagctcctc ggcgccgaac tcctcgatcc ggaaccggcg gacggcgtcg
    21061 gcccggccgc cgtcggcgtc gggcccgagg acgcgcccgg ccggagtcgt caggcgggtc
    21121 tcgagctccc tcccggcggc gccgtcgccg gcgcgcagcg cggcgtccgg ctcccccgcg
    21181 gggaggaccc agacgaggag cgtgccggtg ccggtcgcgg gaaggtcgac gacggcgcgg
    21241 cccccgtcga ggagggccgt cttcacgaac gtacgcgagt cggcttcggg cgaggcggcg
    21301 gcgaacggcg cgaccgtctc gcccggcgcg aagcggccga cggcgacttc gccgccggcg
    21361 agcggcgcgg cggcgacgag tgtggcgagg agcgtggaga tgaaggtccg ggtcttcacg
    21421 tcggcctcct cagtacgtcc cggcgagcga ggtgccgagc ttccggtagt cgttccggcg
    21481 gttgtggtgg tggttcgcga gcgtcaggaa ccagttcgtc ccggctccct gcgcgctgta
    21541 ctgcgagacc atcccgtcgt cctcgcccgg catcccggcg acgccggaga gcgtcgcgag
    21601 gccgtagtcc tcgccgtgcg cgaagtcgtt ccagaggccc gtcccggcga cgttgtagta
    21661 gggcttcgga agggccggac gaccggcgct ccccttcagg tagtactggt tgtagtaggc
    21721 catcgaggtc gtcgtgcagt tcttcgtgga gtcgttgttc tgcccgacga ggctgacgag
    21781 ccacccggtg agccacgagc cggagagggt cccggcgagg ttcgcggctt ccgagccgcg
    21841 gttcggtggg gcgagcgcgt tgacgcgctg gatccggctg aggatcgccg ggtagcgcga
    21901 gtcgtacgtc gggttcgaga ggatccaccg gaggacgacg ccgccgaacg agtgcgtcac
    21961 gacctggatc tcgtcgaccg cgttcgcgtt catccaggtc gtgatctggc cggcgacctg
    22021 tcccgcggcg acccacatgt attgagtgcc gtcgtagtgg cagacgaggt aaggcaccgc
    22081 gtaccccttc gtcgtcgccc ggatcatgtc cgagccccag taggcgttcg cgacgctctg
    22141 gttcgcgagg tcgctccccc ccttgccgtg gacgaagacg actcccttct tcgcgagggc
    22201 gggagacggg accaggagca gggccgccag agcgagcccg aggacggacg ttcgcattcg
    22261 atacctcctc atgccggtcg gttcccgagc cgagcgcaag gtcggtgccg cacagcctcg
    22321 cgcgcgttcg gcgggccgga ggcggcctgg aggcgcgttt acgcgggcgt tcgggggtag
    22381 gccggtgacc cacgcgtcat cggctgaccc tccgccgcaa tccggacggg tccggtcctg
    22441 atccgtccgg gcgtccgtgc gatccggaac ggagcctcag gcggcggcct cgctcccggc
    22501 gggcgtcggg ccgacggaga ggacgacgag ggtctggtcg tcctcgggcg ggcggttccg
    22561 ggtgaacctc cggacggcgg agaggagcgc ctgccgcatt ccgtcggctc cttctcgcgc
    22621 cgcgcccgcg aggacctctt cgaatcggcc gaagccgaac ggctcgccgt cgtcgtcgag
    22681 cgcctcgatg agcccgtcgc tgtagagcac gaggcggtct cccgggccca ggtgggtcgt
    22741 gtgctcgacg aagggggtcc gccgttttac gccgagcggg aagccccacg cggagagcgg
    22801 ttcgggcgcg cctccacccg cgcggacgac gtagggatcg agatgcccgg ccgaggcgta
    22861 gcgaaggtct cccgtcgcgg ggtcgaggac gccgaagaag aacgtcatca acatccgctt
    22921 cggggccgtc ttcctcaccg tctcgttcag cgcctccatc acgcggaccg gcgacgggtc
    22981 gtgccccgcc tggacgagga gcgccgactt cgccatcgcc atcacgatgc cgctcgtcag
    23041 gccgtggccg gcgacgtcgc cgaacgcgag gccgatccgt cctccgggga gagggaggaa
    23101 gtcgaagtag tcgcctccga tttccgtggc ggcgcggtag tgcgaggcga cgacgacgcc
    23161 cgggagcttc gggtcgctcg acgggaggag ggccgcctgg atccggttcg cggcgtcgat
    23221 ctcggccttc acgcgctccc gctcgacgat cctcttcacg tgcggggcga ggtcctcgta
    23281 ccggtactcc accgtccggc gggtcgcgag gcccgcgatc gcaagggcga ggaggaggag
    23341 gagcgggacg agaagcgccg cggccgcgcc ctggagcgcc ggcccctcga gcgagcgggt
    23401 gagggggagg agccccagga ggagcgtggc gccgaagagc gacacggccg aggcgaggag
    23461 gtccttcgcg aggaagacgc cggcggcggc caggggtgcg ccgaagccga ggaggaaacg
    23521 tgtcgtctcg ggcccgaccg gcgcgaggcc cgggacgagg accgagccga cagcggcggc
    23581 ggcgaggagg ccgacccaga ggagcctccg ccggtggaag gcggagaggg tgaagaggag
    23641 tccgacgacg gcggtcggga tcgcgaacgc gagcccggcc agggtcatcg tcgcgggacc
    23701 ggcctcggat tcgaggacga ggttcgacag ctcgccgagg acggggcgag ccccgccgag
    23761 gacgagggcc ggaaggcagg ggaggagggt ggctgcggcc acgaccggcg cgaggaggag
    23821 tcccgtgagg agggcgccgc cgacgctcgc gttgacggcg tcacggcgga ggagcgcgtc
    23881 gaacgaggcg agcctctctc cccagcgctc gcgggcgtag ctctcgccga ccgaccagga
    23941 gaggaagacg agcgtcgcga ggggaaggtc gaggaagagg aacgcgatgg cggcctgggc
    24001 gagcgccgtc tggaacgcgt cggtcccgcc gaagccgacg ttgaacgact gcgcgcgggc
    24061 cacgaggacc tcgagcgcca gcagcagcag gagctgcgcg ccgaagagcc aggctcccgt
    24121 gccgacgccc acctccccgg cgtggtactt ccggaggaag atcccgagga ggacgaagag
    24181 gaggccgaag acgacgacga aacggccgag cgtcgtgacg atgctgccgc ccagctcgta
    24241 gcggaacgcc cggccgtcgc ggtactcctc gtcgagctcc caccccgaga ggcggttgcc
    24301 gacgaaatgg acgttcagga agaacgacac cgtctcgggc ggtccggcgt gagggacgcg
    24361 atagcggacg acgtgatcga cccggtccct gaggacgttc gggcgggcgc cgtcgaaatc
    24421 gaaggagggg gcacccggga aggagcgcga cgccacgaag cggtcggcgg cggcgcgcac
    24481 ctcgtcctcg gtcggccggc ctccgacctc ttccgaccgg gcgcggaccc gtgcgccgag
    24541 gacggtcccg tccttcccga cgaggacgta gccgaactcg gggtacttct cgagcccggt
    24601 ccggaagtag gtgacgcggt aggccccgag gcgcggcccg acgaccgggt cccgctccag
    24661 ggcgaggcgc gtggccgggt cccccgcgat ctcctgttcg acgaggaggc tctgttccca
    24721 ggtctccacc cggaaggacg ctccgacggg gatgccgagg cgggtcgcct cggcatcggc
    24781 gatccggacc gcggcggggc gagtgatcga gaggccgcga ggctgcaccg tctcgaaccg
    24841 cggaagcagc gcggcgaggc cgacggctcc gaggacgcca agggcgaggg cgagcttcgc
    24901 cttcatgctt cgtcaggtac ggacgtgggg ctcccgaggt tcgccggggg gcgcggcatg
    24961 cgggcggatc gtaccttcgg ggcgttaacc tcgaggtcat gaaggtcttc gtcacgcagc
    25021 ccgttcccga gccttcgacg tcggacctcc tttccggttt cgaggtcgtc gtcggccggc
    25081 cggacgcctg gccggaggag gggcgccggc acctcccgga ctccgacgcg ctcgtcccga
    25141 cgccccgcga gagggtgacg gaggagctcc tcgagctcgc cccgcgcctc aaggtcgtcg
    25201 cgaactgcgc cgtcggcacc gacaacgtcg acctcgaggc ctgccgccgc cgcgggatcg
    25261 tcgtgacgaa cacgccgggc gtcctcacgg aggcgacggc ggacctgacc tggctcctga
    25321 tcctcgccgt ggcacgacgg ttccgcgagg gcgacgcgct gatccgctcg gggacgtggg
    25381 acggctggaa gccgatggag ctgatgggcg cctcgctcgg cggcaagacc ctcggcgtct
    25441 tcggccccgg ccggatcggg acggccgtcg ctcgccgggc ggaggcgttc gggatgcgcg
    25501 tcgtcgtctg cgacgccgcc gacggcctgg gggccttcga gcggctcctc gccgagtcgg
    25561 acgtcctgac gatccacgcg ccgctgacgc cggagacgcg cggccgcttc ggcgcggcgg
    25621 agctcggccg gatgaggcgc ggagcgatcc tcgtcaacac cgcccgcggc ccgatcgtcg
    25681 acgaggaggc cctcgccgcc gcgctggcgt ccggccacct cggaggcgcc gggctcgacg
    25741 tctacgagga ggagccggcc gtccacccgg gcctcctccc tctcccgaac gtcgtcctcc
    25801 tcccgcacct cggctcggcg acgcgcgaga cgcgcctcgc gatggcgcgg acggcctgcg
    25861 ccgaggcggc gcgcgtcctg tgcggcgagg agccgcggca ccgggtcgcc tgactcagga
    25921 cgaggagacg tccacccgcg ggaggcgccg cgcggccgcg agcatcgccg cggtcgcgag
    25981 cgcggccgag gtcgcgcccc acgcggcgag gaggagccgg ccgtcgaggc gtgcgccgag
    26041 gagcgtctcg gcgaggccgc ggagctcctc gcttacgacg aggaccgggg cgagggagag
    26101 gagcgtcgag agccccatcg cgacgaggcc gcccggcccg agggcgacgg aaacggggtt
    26161 cggcgcgtcg tagcggggga agagcgcgcc gagcccgagc tggagcgccg ggatcgcgag
    26221 cccgccgact gcggcgaccg cgagcgcggc gccgagaggg aagccgcgag cctcgagggc
    26281 ccatgcggag gctcccgcga ggaggagagc cgccgccagg gacggggcgg cccggaccgt
    26341 ccacctcacg acgaggtggc gcgcggcgga gaggggaagc gacttcagga ggagcgccgc
    26401 acggccgtcg gtcgagacgg cggggaaggc gaagcggagg gcgagggccg agacgaggaa
    26461 gaggccgagg cccgattgca ggccgtggac gaggtcgcgc gaggcggcgt cgcccgaggg
    26521 aaggagcttc aggttcagga ggtcgagtac gaagacggcg gcgagcgtcc cgagctgggc
    26581 cggagtcgcc gcgtcgcgca ggagcgtccg gacctccgcg cggaggagct cgcgctcggc
    26641 ggaggccgcc tttctcccga cccgccggcg cggcgtggcg tccgcctcct cgcggctccg
    26701 ccggaggagc cggaggtgaa gcggagcgag gagctgagcg aggaggacgg tgagaagcgc
    26761 cgcgccgccg aggaggagga gggcgtggag aaggccggca gcgtctcccg cgagggcgcg
    26821 cgcggcggcc cgggcggcga tcgccacggg gttcccgccc ggctcggcga ggggcgtggt
    26881 cccggcgcgt aggaggaggt cgagcgctgc ggaggggtcg aggaagcgct cggggcgtac
    26941 cccgcgtgcc ccgacgatcg ccacggcgag gcccgtcgcc gagaggaacg ccgcgaggag
    27001 gcgggcccgt cgcggaggga agacggccac gacggcgagc gcgagcgcgg agccggccgc
    27061 gcccgcgagg acggcgacgg cgaggaacgc ggcgccaaga gccgcggccc cctccgcgcc
    27121 ggagcgcgcc gcgaggacga cggccggaag ggcgacgagc gccgccggcc ccgaggcgag
    27181 cgcgatcgtc gccgcgacct gccggaggag gagccgcgcg tgcgggatcg ggagcgccat
    27241 ccggaggagg agctcctcgg agaggaagag cgtcgagacc gccgtcgtca gggcgccgag
    27301 gaggaggagg actcccgcgc ccgcgaacgc gccgaggagg agccgttcga ggaggatctc
    27361 cgggacgagc gcggggagct gcgtcgccgc gctccggccg agtcgaagga ggtgcgcgag
    27421 ccccgcgtac tcaccgacga cgaacgcccc cgagaagagg cccaccgcga ggactcccgc
    27481 ggcctccgcc gtcgagcgcg gcgagagcga gcggccggcc gcgcggaggg cgttcgcgaa
    27541 ggagaccgcc ccggcgctca cggctccccg ccgtccgcgg cgaggtcgaa gaagagctcc
    27601 gagagccgcc ccgtcgcccc gcgcgcgctc accgcgagcg gcgtgccgcg cgcgacgagc
    27661 cgccccttca caaggacgcc cgcctcgtcg gcgacctcct cggcgagggc gaggtggtgc
    27721 gtcgtcagga ggacggcggc gccggccgcg gtccgctcct tcagacgctc ggcgaggcct
    27781 ttctgcgcga gcgggtcgag gccgaccatc ggctcgtcga ggacgaggag cgacgggtcg
    27841 tggaggaagg cctgcgcgag ggcgaccttc tgccgcgtcc catgggagag cgtctcgttc
    27901 gtccggtccg ccgcggcgcc gaggccgaag gcctcgagct ccgcctcgat cctctcttcg
    27961 agcgcggccc ccttcgcccc gaagaccgct ccgacgaacc ggagcgtctc gcgcggcgtc
    28021 cagcggggcc agaggtacgg gcggtcgggg acgaacccga gccgccgctt cgcccgaacc
    28081 gggtcctcct ccacgtcgat tccgaagacg gtcgcccgcc cggccgtcgg cgcgaggagt
    28141 cccgtcagga ggccgatcgc ggtcgtcttc ccggccccgt tcggcccgac gagcgcgaag
    28201 acgacccccg gcggcacttc gagcgtcagc tccgagaggg cgagcaggtc gccatagagc
    28261 ttcgtcgcgc gctcgaaggc gaaggcaggg ggggcggggg ccactggccc catcgtacgg
    28321 gaggtcgagg gcgccagccg ttcgaccgat cggaaacccc ggccccgcgc ccgtcgtaga
    28381 gtcggcccga caaggaggag cgatgcgctc gatgtccgcc ctggcgattc ccgtcctgac
    28441 cctctcgttc acggccgtcg cggccggtcc ggccgtctcg ccggccccgg cgggcccgag
    28501 accggtcgac ccggcctcgt tcgacccgtc cgtgaagccg tgcgaggact tctaccagca
    28561 cgccgtcggc ggctggctga aggcgaaccc ggttcccgcc gacaagtcgc gctggggctc
    28621 cttcgaggag ctcgccgacc gcaaccgggc ggtcctgaag gcgatcctcg aggagacctc
    28681 ggcgaaggcc gactggccga aagggagcgt gcggcagaag gtcggcgatt tcttcgcctc
    28741 cggcatggac gaggcggcca tcgagaaggc cgggacgaag ccgctcgagc cctggctcgc
    28801 gaaggtcgac gccctgaagt cgagcgccga cctcccgaag ctcatggccg agctccacgg
    28861 ccgccggctg atggcgggct acgggttcta cgtcggtcag gaccagaagg agacgacccg
    28921 ctacctggcc atgctctccc agggcgggct cggcctcccc gaccgcgact actacctgaa
    28981 ggacgacccg aagtcgaagg agctccgcga caagtacgcc gcgcacgtgg cgcgcatgct
    29041 cgagctcctc ggcgacgctc cggaggcggc gaagacgaag gccgcgtccg tgatggcgct
    29101 cgagacgaag ctcgcgacgg ccgcgcggac ccgggtcgag ctgcgcgacc cggagaggaa
    29161 ctacaacaag cggaccgtcg ccgagctcgt cgccgccgct cccggctacg actgggcggc
    29221 ctacttcggc gcgctcgggg ccgcgggcct gaaggagctg aacgtccgcc agcccgagtt
    29281 cgcgacggcg ttcgcggcgc tcgccgcgtc cgagccgctc gacgcgtgga aggcctactt
    29341 ccgctggcac ctcgtccgcg cgtcggccga cgtcctgccg aagcggttcg aagaggaggc
    29401 gttcgcgttc tacggccgga cgctgaacgg cgtcccgcag caggaggagc gctggaagag
    29461 ggtccaggcc gcgaccgacc gcgcgctcgg cgaggcgctc ggccagatct acgtcgagcg
    29521 ggcgttctcc ccgaagtcga aggagcggat gaaggtcctc gtcgagaacc tccgcgcctc
    29581 gctgaaggaa cggatcgcgg ccctcccctg gatgggagac gagacgaaga aggcggcgca
    29641 gaagaagctc gcggcgttcg gtgtgaagat cggctacccc gacgtgtggc gcgactactc
    29701 ggccctcgag atctcccgcg cctcttactt cgagaacgtc gtcgccgcga acgcgttcga
    29761 ggcgaagcgg aacctcggca agctcggcaa gccgatcgac cggaccgagt gggggatgac
    29821 cccgccgacg gtgaacgcct actacagccc gacgatgaac gagatcgtct ttccggccgg
    29881 gatcctccag cccccgttct tctgggccga cggggacgac gccgtgaact acggcggcat
    29941 cggcgtcgtc atcgggcacg agatgacgca cggcttcgac gactccggga gccgctacga
    30001 cgccgacggg aacctgaaga actggtggac ggaggaggac cggaaggcct acgagacccg
    30061 gacggcgctc gtcgtgaagc agttcgacgg gtaccggccg ctggcggacc tcggcgtgaa
    30121 cgggaagctg acgctgggcg agaacgtctc ggacctcggc ggcctcaagg tcgcctacga
    30181 ggcgctccgc aaggcgctcg ggacggaccc gaacgcggcg gagaaggtcg acgggttcac
    30241 gccggcgcag cgcttcttcc tgaactacgc ttcggtctgg aggaacaaca tccgcgacga
    30301 ggcgctccgg gtccggctca acaccgactc gcacagcccg ggccgcttcc gcgtcctcgg
    30361 accgctctcg gccctcccgg agttccacgc ggctttcggc tgcgcggccg ggagcccgat
    30421 ggtgatgccg gaagcggagc gcccgagcat cttctaggcc tcgccccgtc ccgaaaagag
    30481 aagcgggggc cggcgatgcc ggcccccgcg ggggtgggag acgcggcggg tgcgccgcgg
    30541 gagggacggt cagcggtcga gcttcgcggg gaagatctcg tacgggagga ccccggggag
    30601 cgtctccgga cggacggtcg agaggtaggc gaggaacggc tggtcgccgg tgatccggaa
    30661 ccaggctccc cccgcggagc agaccggaag gtcctcgagc gagaggtcgt tgaactggac
    30721 gatcgacgcg gcgggcacgg cgtactcctt ctcggcgggc aggaggccgg ggcccggagt
    30781 cgccgacacg gcggagacgc ggaacgtggc cggcgcatcc cccgcgttga agagcgtcag
    30841 gttcacgcgg cggcggctcg tcgcgccctc ggcgcactcg gaggcggaga gggagacgtc
    30901 gccggcgacg gcggtcatcc cggccgggaa gagctcgccg aagaccggga gctcgaggcg
    30961 gccgagcgac tcggggacct tcgcgagacc ctcgggcacg gtctcgaggc gctcgagcgt
    31021 ggcgcggagc gccacgcgcc tgggcgccgt gatcgcgagc atctggacgt gctgtcccgc
    31081 gcggagcgtg tgcgagtcga ggacggtggc gccgttcggg ccgacggtgg cgtcggccgg
    31141 aggctccgcg gcgccgagcc cgtcccaggc gcccgcgaac gcgatcgtga cgtccttgtc
    31201 ggtgttgttg tacgcggcga gggtgctcgc catcgagacg ctcgcgccgg gagcgaagaa
    31261 gcccgggacg tagacggtgg cggcggcgag cgggacgacg gaaatcgagc cgctgccgat
    31321 cggctcgggg gcggtgaacg tccgggtccg ggtgacctcg acggcccagg tcccggcggc
    31381 gagggggccg accgggacgt cgagcgccca gggcgtgacg gccgcggtgc acggtccggg
    31441 gatgccggag tagttgaagt tgacgtgcag cttcgtcccg gacgtcgtca gcttcgtccg
    31501 ccccgtctcc gggacgcagc cgtcgggcca ctcgcccgag agcttcagga cgatctggtc
    31561 gccgggcgcg ggattggccg ggaacgtcgc gatcgacacg ctcgggagcg tctgggcggc
    31621 ggccggggac gcgacagcgg tgagggcgag gaggaggatc gggagcgtcg tgtggtgtct
    31681 catgcattca ccttccggtc cggagtgccg cgacgcgcgg ccgtcccacg cgcggaggcg
    31741 cggtgtggga ttcacgcgcg tcgcgtcatt tccctcgttc gggcctgtgg cacgatcgcg
    31801 gggtggacgc cccggacgcg ccgaccgacg cggacctcgt cgcccggatc ctccgggggg
    31861 aagaggagct cttcacgctc ctcgtcgacc gctacaagct gcgcctcttc cgcttcgtgt
    31921 cgcgttacct cggcgacccc gaggacgccc gcgacgtgac gcaggaggtc ttcctcaagc
    31981 tctattccgc gctcgactcc ttcgacccga ggtatcgctt ctcgacgtgg gcgttccgga
    32041 tcgccgggaa cgccgcgatc gaccacctgc gtcgaaggag gatccgcgcg atcccgctgg
    32101 agcttcccgc cgacgacgag tcggaccgcc ggtcgatcga tccggtcgac ggcaagcccg
    32161 acccgctcga ggagctctcc cggacgcgcc tccgggaggc cctcgacctc gcgatcgaga
    32221 ggctcccgga cgactaccgg gagctgatca gccttcgcca ccacggggag ctggcctacg
    32281 aggagatcgc cgagctgaag ggaatgcccc tcggcacggt caagaacaag ctcttccggg
    32341 cccgccaggc cctgcgagat cttttgccga aaggcaccgt atagagagat cgggacgatg
    32401 acgatgcacg cgaggaccac cgacgggaac ccgacgccag ccgagctcga cctcgatcgg
    32461 cgaatggaag tgtttctgga cgagatggtt agctcttccg atcgagagac gctgccggcc
    32521 gacttcgtct ctcgggtcgc ggcggcgcgg ccgtttgcac cgtgggaggt cgcccggggc
    32581 gccttttggc gtcttccggt cgccgtcggc ctcgggctcc tcgccgggag cctcggtctc
    32641 gccctgaccc ccctctggag cctcggcccc gcgacggcgg tgacggtctg ggcggagctt
    32701 ctcgcggtcg ccttcggaag cccggtcgcg accctcgtcg ccgccctccc gctcctcgcg
    32761 gaggggaccg agcgggcggc gggagccgtt tcgcccggcg ccgtggcgct cctcggcggc
    32821 gcggcggccc tcgtggccgg ctccctcctc ggggcgctcg cccgattccg ccggccggag
    32881 ctcccggccg cccgctcccg gagctgagcc ggggccagcg atggcccggc gcctcgtcct
    32941 tctcctgctt ccggtcctcc tctcgagcgc cacgggcgtt gcggctgagc ccgtcgacgt
    33001 ccccaacggc gagttccgct ccggcgacgt cgtcgcgctc ggacgggagg cacgggtcga
    33061 gggggtcctc cggggaacgc tcgtgacgat cctgggggac gtccggatct ccggacgggt
    33121 cgaggaggac gtcgtcgcgc tcggaggaaa cgtgcacctc gagccgggag ccacggtcgg
    33181 aggggacctc ctcgccctcg ggggagaggt ccacgcaggc ggcccgggtc cggcgcctgt
    33241 cgggggacgg ctcctgaccg tcggagcgct cgaagccgcc ttcgccgcgg agctgaagac
    33301 ctccccgctc gcccttcggc cggtccgggg cctcttcctc gcgttccggc tcgccctcct
    33361 cctcggctgg ctgatcgtcg gcctcgtcct gctccgcctc gttccccggc cgctgaccgg
    33421 cgcggccgag ctcgttccgg gtcggctcac cgccgtggcg gcgatcggcg ccgcagccgt
    33481 ccttgccgcc ctcctcgtct ccgcgctcct cctcgtcgtc cttccggcga ccgccggcct
    33541 gttcgcggcg ggcctgctcg tcgtggcgct cgccctcgcc aaggcgttcg ggatcgccgc
    33601 cgttttcgtc gccctcggga ggcgcctgac gcggacggcc gcgaggggga gcctgctctt
    33661 cggcgacccg gcggctctcg cggtcgggct cgccctcctc ggaagcgttt cgctcgtccc
    33721 cgtcgttggc ccgctggcct ggtcgatcgc ctcgctcctc gggatcggga cggccctcgc
    33781 ggcggcggca cgcccccggg ccgcggcctt ctgaaggaaa tttccgcctg atacgctccg
    33841 tcggccgagc ccgcgcggga cgcggaccgg cggaggagcg gtgatggcga tcgagcgcgt
    33901 cggggtgatc ggggcgggtc agatgggggc ggggatcgcc cacgtggcgg cgctggcggg
    33961 gaagaccgtc gtcctcgccg acgtcgacgc ggcgctggcg gaaaaggggc tcgcggtcgt
    34021 ccggaagaac ctgggacgcc aggtcgagaa gggaaaggtc acggcggaag cggccgaggc
    34081 cgccctcgcg cggatcgccg tctcgggcga cctcgccgcg ctcgcggacg ccgacctcgt
    34141 cgtggaggcg atcgtcgaga acgaggaggt caaacgcggc ctcttctcgc ggctcgacgc
    34201 gctcctccag ccgggggcga tcctcgcctc gaacacctcc tcgatctcga tcacgcggct
    34261 cgccgccgcg acgaagcggc ccgagagcgt catcgggatg cacttcatga acccggtccc
    34321 ggtcatgcag ctcgtcgagg tcatccgcgg catcgcgacc tcggacggga cgacggaagc
    34381 ggtcgtcgcg gccgcgaagg agatggggaa ggtcccggtc gagtgtcgcg acttcccggg
    34441 cttcgtctcg aaccgggtcc tgatgccgat gatcaacgag gcggtcttcg ccgtcttcga
    34501 gggggtcgcc acgcccgagg cggtcgacac catcatgaag ctcggcatga accacccgat
    34561 ggggccgctc accctcgccg acttcatcgg cctcgacacc tgcctcgcga tcctgaacgt
    34621 ccttcaccgc gggctcgggg acgacaagta ccgtccctgt ccgctcctcg tccagatggt
    34681 cgatgccggc tgggtcggca ggaaggccgg gcgcggtttc taccgctatt cggggcccgg
    34741 cggggagaag gtcgcgggtt gatctgccag gtctgcggcg cgtcgaacga cctcgacgcc
    34801 gagctctgcc ggaagtgcca gaacaagctc ctcgtcgtgt ccggctcgag cgagtcgtac
    34861 gacgaggggc cgtcgggcga ggggatctcc ctcgacgagc acctgctcga gagggtctcg
    34921 gtcctggagg agatcgtcaa gcggagcgcg gagacgctga aggcgctcct cgagggcttt
    34981 caccgccagg agaagaacgg cttcgtcgtc cagacgggcc tcctcgcgct gaaagacctc
    35041 ctcgagagga aggggctcct cgtcgagcag gagttcgtcg acctctggga ggagcgcgtc
    35101 gaccagcaca tgaccgttct cgagaagcgc gagcgcttcc tcgagcgcaa ggagcggatc
    35161 ctcgcggagc acaaggggct cgggcgggaa cgcctcgtcg acctgctcgg ggaggcggag
    35221 ctcgccttct ttgcgctcga ccccgacaag gcgatggccc tcctcgagga ggcctggaag
    35281 ctcgaccgcc gcaacgccga gctcgcgttc ttcctgggcg agacctggtt caacgacggg
    35341 gactccggaa aggcggcctc ggccctccgg catgtcctcg agcagcgtcc ccagcacttt
    35401 gacgccctcg tctactccgg ggtcctcgag aacgacctcg gccgcccgga gaaggcggtg
    35461 gacgccctga agcgggcgct cgccctccgg cccgacgcgt tccttcccgc cttcgcgctc
    35521 ggcgcgatcc acgccggggc gggccagctc gcgaaggccg aggcgctcct gaagagggcg
    35581 gtcgcgatcg aggagaaccc ccaggcgctc tcgctcctcg gcacgatcgc ctaccagcgg
    35641 ggccgtctcg ccgacgccat tgacgcgctc cagcgcgccg tgcagctcga ccccgacgac
    35701 gaagaggggc tcttccagct cggactctgc tatctcgacc gggggtggac ccagaaggcc
    35761 acggagcgat tccgcgcggc gctcgagctc agcccgaacc ggatcgagct gcaggaggcg
    35821 aagaagctcc tcgggccggc cggcgcgggg gcactgcccg gtctcgccgg gacggtcggc
    35881 gagcggggcg ggcgcacggc cgcggcggcc gcgcgggacc cgcggaaggc gctcggcctc
    35941 tatcgccggg cgatcaaggc ggacccggag aacccggcgg tccagatcgc ctacgccctc
    36001 gtctgctcct ccgtcggccg gacggcggag gcggaggcga cgacgcgccg tgttctctcc
    36061 cagaagcccg atgaggagat cgccgccgcg gcctggtcga cgctcctcga gacgctccgc
    36121 tccgagggga agttccggga agggacgcag gtcgcgggcg agatgctcca gtccgtcacc
    36181 tcgagctcgg cccgttcgat cgcctacttc cagcgggccg ccgccctggc cgacatgggg
    36241 gagaggctcg acgaggcgct cgaggacgcg gagaaggccc tgcggctctc tccccgggag
    36301 ctccgccact tcccgctcgc gacgaagggg tgggtccact acaagcggaa ggagttccgc
    36361 cgggccgtgg agtgcctctc gcgtgccgcc gagctcgggg agacgccgac ggcgctcgcg
    36421 catctcggcc tcgcgtacct cgccgtcggc gacgcgaagg ccgcccgcag cgccttcgag
    36481 cgcgccaagc ggcggggcgg acgaccgtcc gagcgccacg gggggctcga ggcgcggatg
    36541 ctggagcaga tccggcggaa cctccagctg gcggagcggg tcgccagccg gccgggccgc
    36601 cgaaagcccg ggccgggctg aatcgcgtcg gctttccgtt cgatttctct tgacaggctt
    36661 cctcgaaggt cccgagaatc gtgccaggaa aacataccga gtgttcggta ttttgttaga
    36721 ggcgacgcga tcgaagcgcg agcggggccc gggaacgggt cctacgcttc gcccgctccc
    36781 cgcgagctgc ggctggccac tgggttgacg agggcgcgac ggcgcctggg aggatcgaga
    36841 tgacgaggct gacccgaaac cccttccttc gcgccgcggc tctcctcggg gccgttctcc
    36901 tgcccgccct gacctgggcc ggccccgcac gggcgcagac ggccgacgcg accgtcgagg
    36961 tcgacgtcgt ggacgagacg ggcgtcgcgc tccccggcgt gtcggtcgag ctaaagcgcg
    37021 ccgagaccgg cttcgcccgg acgtccgtca cgtccgccgc gggcgccgcc cgcttcccgg
    37081 cgatcccgcc ggccgccggc tacgtcgctc gcgtcgccct ccagggcttc gaagccgtgg
    37141 agcagccgct cacgctccga atcggacaaa cggcgcggct gcgcgtcgtc ctcagggcgc
    37201 aggcggtcac cgaggcggtg acggtcgtcg gcgaggctcc gctcgtcgac gtctacaaga
    37261 tcgactcctc gacgaacatc gtccccgagc agatcaagga gctccccgtc gcgaaccgcg
    37321 agttcgaaaa gctcgcgttc atcgcgccgg gcgtccagcg tgagcggggc gagttccggt
    37381 tcgtgacggg cgggccggtc atcggctcgg gcgggaacgc ctcgcagtcg acgatcctcg
    37441 tcgacggcgt cgactacacc gacccggccc tcggcctcgc gaagacgcgc gtctcgcagg
    37501 acgcgatctc cgagttccgc gtcgtcaact cccggttcga cgccgaggtg ggcggctccg
    37561 ccggcggcgc cctcacggtc ctgacgaaga ccgggacgaa cgagctgaag ggctcgctct
    37621 tcgccttcta ccgggccgaa gggctccggg cgcagggggc cctcgagcag gactcctcgg
    37681 ttgacttccg acgtggccag tacggggtca cgctcggcgg cccgatcgtg agggaccgga
    37741 cccacttctt cctctcaggc gagtacgtcg acgaggtccg gccgacgctc taccggccgc
    37801 agggggcctt cacggcccag gccgccgacc tcgaggtccc gatggaccag ctcctcgcgt
    37861 tcggctcgat cacgcactcc ctgtccgact cccagacgct cctcgtcaag gccgacctcg
    37921 agcggtaccg ccaggacaac ttccgcgtcg ggggcgtgca ggacgcctcc tacggccagg
    37981 agctccagcg cgacaactac aacttcacgg gaggccacac ctgggtcgtc ggcgccgcga
    38041 cgacgaacga gctccgggcc cagatcggac accggaagta cttcgagccg acgaactccg
    38101 acgccgtcgc ggactggttc acgaacggcg tcaccctcaa gacgggcggg aacatcctcg
    38161 gcgacctcct cggcgagggg gaccagctcg agatccgcga caccctctac ctccacctga
    38221 ccgggaagag cgggacccac gacgtgaagg tcggcgccgg ctggcagcac gtgaacgacc
    38281 gctcgatcat cgacacctac gcgagcggcc tcttcctctg ggcgggagac acgaaggcct
    38341 tcccgatcgc ctacggctac ggcgtcggct cgagcgacgt cgagatcacg acgaaccggc
    38401 tcgccgcgtt cgtccaggac gactggcggc ccctctcgaa cctgaccgtc agcgtcggtc
    38461 tccgctacga cctcgacacg aacgggaaca acgagggctt cacgcacccg ctcgtccccg
    38521 agccgcgcga gagggacacg aacaacgtcc agccgcgcct gggcctctcc tgggacgtga
    38581 agaaggacgg tcgcttcgtc gcgcgcgccg gctggggcct cttcgtcggg cgcaacctgc
    38641 tcgtccccgc gttcaccgag ctccagcaga acggggtcac gggccgcaag ctctacaccc
    38701 gcgccaacgg cctcctctac ggcctcccgg ccttcgccct cgacccgagc aacccgatga
    38761 cgacggggct tccgctcccg atcgacatca cgctcctcga ccaggagctc gtcgcgcccg
    38821 aggcgagcca gttcagcgcg ggcctcacct cgcggctctg gaagaccggc ctctacctcg
    38881 acgtcgaagg gatctgggtg aaggggagga aggagctgat cgtccacgac gtcaactgga
    38941 acgggaacgc gaacccgacc cgtccgaaca aggcctacaa ccaggtcaac gtctactcga
    39001 acgacggcga gtccgagtac aaggcgctca ccttcgcgct gaacggcagc ctcaagggcg
    39061 gccacctcgt cacgggctcg gtcacgttct cgagcaagca caaccgctcc gacgacttca
    39121 gccccgagtt cccgacgggc tacccgagcg accccgcgga ccttccgaac gagtggggcc
    39181 gggggcgctc cgcggagagg atgcggttcg tcctctccgg cgtcttccgc cttccctggc
    39241 agctcacggt ggcgccgatt ttcaactacg gctcgggcca gccgtggacc cgccgctacg
    39301 gctacgactt caacggcgac ggcaagacgg gcgaccggat gcccggggtc gaccgcttcg
    39361 gcatggacgg cccggtctac cgcagcttcg acctgcggct cacgaaggcg ttcgagctcg
    39421 gctcggccgg caccctcgag ctcgtggccg aggcgttcaa cctcttcgac tccacgaact
    39481 acgacgtcgc ctcgatcgac ggcgccgagt tcctgagcgg gccgaccctc gccaacccgg
    39541 cggcggcggc ggtcgcgaac ccgaactacg gaaagccgag cgcgacgctc ccgtcgcggg
    39601 agttccagct cggcctccgc ttcgccttct aggagccctc tcgtcctcag ccgtccggcg
    39661 ggcgcgccgc gcccgccgga cttcccgccc cgcctccggg ccgggcgacg ccgggggccc
    39721 gagggggccc gcgcagtggg aggttctgcc atgagcatcg aaacgggact cgccggacgg
    39781 tccgtcgtcg tcaccggagc ggccgccggg atcggaaagg cgacggccct gcgattcgcg
    39841 aaagaggggg cgaaggtcgc ggcgtgggac gtcgcggacg ccgccgcgct cgccgacgag
    39901 ctgaaggccg cgggcgccgc cgacgcgttc acggcgaagg tgaacgtcgc cgacgccgcc
    39961 tcggtggagg cggcggtcgc ggacgtcgtc gcgaggtggg ggcggatcga cgtcctcgtc
    40021 aacaacgccg ggatcgtccg ggacgcccag ctcgtcaagt acaaggacgg ggccgtgctg
    40081 gcgacgatga cggacgagca gtgggacgcg gtcatcggcg tgaacctgaa gggtgtcttc
    40141 ctctgcaccc gcgcggtgac gccgcacatg atcgcggccg ggggcggcgt cgtcctgaac
    40201 gcctcctccg tcgtcggcct ctacggcaac ttcggccaga cgaactacgt cgccacgaag
    40261 gccggcgtcg tcgggatgac gaagacctgg gcacgcgagc tcgggaggtt ccggatccgc
    40321 gtcaacaccg tctgccccgg gttcgtcgcc accgagatcc tgaaggcgat gccgcagaag
    40381 gtgctcgacg gaatggtcgc ccacacgccg atcggccgga tgggggagcc cgacgacatc
    40441 gcgaacgcct acgtctggct cgcctccgac caggcgacct tcgtccacgg cgccgtcctc
    40501 tcggtcgacg gcggcctcgt gacggggacg tagcgatgcc ccgcgtcgcg cgcatcgtct
    40561 cgacggcccg gtacctcccg ccgatcgagg tcccgaacga ggtcctgaag ccgcgctggc
    40621 cccagttccc cgacttcgtc gacaagatgg aggcctcgag cggaatccgg acccgcttct
    40681 acgcccccga ggaccaggcg acgtccgacg tcgccctccc ggccgcgaag ctcgccctcg
    40741 agcgggctgg cctgacgccg ctcgacctcg acctcatcat tctcggcacc gactcgcccg
    40801 actacgtcac ccccgcgacc tcggtcgtcc tccaggggaa gctcggcgcg aagaacgccg
    40861 ggaccttcga cgtcggctgc gcttgcgcgt cgtttccgac cgggatcgcc gtcggctcgg
    40921 gcctcatcgc cgcgaacccg gcgctgaaga acgtcctcgt cctgggcgtc tacctcatgc
    40981 acaagctcgc cgacccgaac gaccccatgg tcttcttcta cggtgacggc gccggcgccg
    41041 cggtcctgca agggggcgac gagcccggct tcctgaactc cgcgatgcag gcgggcgggg
    41101 agtacgcgca gtgctggggc atcttcgcgg gcggcaccgc cgagccggcg accgtcgagg
    41161 ccgtgcaggc cggccggacg acggtgaaaa tggtcgagaa gtaccccgcc gagatcaacc
    41221 acgaggggtg gccgcgcctc gtgaggcgcc tgtccgcgga gggagggttc gccctctccg
    41281 agatcgacca gctcatcttc acgcaggtgc gaatgccttc catcgagctc gtcatgaacg
    41341 acctcgggct tccggtcgag aaggcccaca cgatcatgga gaagtggggc tacaccggat
    41401 cggcctgcat cccgatggcg ctcgacgacg cgatcgagca ggggaaggtg aagaaggggg
    41461 acctcgtcgt catgatcggc tccggcgtgg ggtacaacca ggccggcgtc gcgatccgga
    41521 tgtgacccgg agccgcagcc gcggcgcgga ggtgagatcg tgaaccgacc catgaagctc
    41581 ctcctcgctg cggccgctct cctcgtcctc gcggcgatcg tcttcatcct ctgggcgcgg
    41641 ctgcggcccc tcgcggcgta cgccgcggcg gggcgtcgcg cgctcgcgaa ggcgggtctc
    41701 gagacggccc gcgtcccgtc gcccgcgggc gagcagacgt acttccgcgg cgggagcggc
    41761 ccggtcgtcg tcctcctcca cggcgcgggc gaccaggcct ccgcctggtc tctcgtcgcc
    41821 ccgaagctcc ttcccggacg gacgctcgtc gtccccgacc tcgcgggtca cggcgcgagc
    41881 gcgccggaga agggcgcgat ctcggtgccg atgctcctct ccggcctctc ggccgtcctc
    41941 gacgcggcgg ctcccggtgg gaaggtgacg atcgtcggca actccctcgg cgcctggctc
    42001 gcctgcctct gggcgaagga gcaccccgag cgcgtcgagc gcctcgtcct cgtcaacggc
    42061 ggcccgctga agcacgcggc ggaggggatc acgctcctcc cgaaggaccg ggccgaggct
    42121 cgggccgccg tcgagaagct ccgcgacccg ggctcgatcc gcgtccccgt cttcgtcctc
    42181 gacgacgtcg tgaggcaggc ggcgacgggc cccctcgcgc ggctcgcggc gacgagcggc
    42241 gcgatggagc cgcacgtcct cgacgggaag ctgggaggac tcgacgtgcc ggtcgacctc
    42301 ctctggggcg cttccgacca gctcctgccg ctctcctacg cggagaggat gcgcgtcgag
    42361 ctgccgcgcg cccgcgtgac cgagctgccg cggtgcgggc acgtcccgca gcaggagtgc
    42421 ccggccgcct tcgcggccgc cctcgggaag ctcctcgacg gtccgccgcc ggagagggcc
    42481 gcggccacgc cggaggtccc gtgatccacg ccgacgtcct cggcgagcgg gcgcgcctct
    42541 cgccggagaa gtgcgccctc gtcttcgtcc cgacggggga gcggttcacg taccgcgagc
    42601 tggacgcccg cgcgacacgg gtcgcggcgt tcctccgcga cgggctgggc ctgatgaagg
    42661 gggaccgagt cgcccttctc gcccacaatc gggtcgagta cgtcgacctc ttcttcgccg
    42721 cggcgaaggc ggggctcgtc ctcgtcccgc tcgggacgcg catgacgccg cacgagctcg
    42781 ccttcatcgt gcggaacgcc gacgtctcgg cgctcgtcta cgggggcgag ttcgccgaga
    42841 ccgtcgcaga gctccgcgcc gaggcggcgg tgccgcggcg gatcgccctc gacgcgccct
    42901 ccgcggcggg agacctgtgg ctcgcggcgg agctcgccgc gctccccgag gcgcccttcg
    42961 tgcggacgcg ctgcggcggc gaggacctcc acctcctcct ctacacgagc ggcacgacgg
    43021 gcaagcccaa gggggtcatg gtcccgcacc ggatgacgtc ctggaacgcc tggaacacgg
    43081 ccctctgctg ggggctgacg gagcacgacg tcagcccggt cttcacgccg ctctaccacg
    43141 cgggcgggct cggcgcgttc ctcctcccgg tcctcgcggc gggagggacc gtcgtcctcc
    43201 acgcgggctt cgacgcggcg gatgtctggg ccaccgtgac gagggagcgc tgcaccgtcg
    43261 tcctcggcgt cccgacgatc tggaagctgc tcctcgagca cccgtcgttc gagcgggccg
    43321 acctctcctc gatccgctgg ctcatcagcg gcggcgcgcc gctcccgctc tacctcatcg
    43381 aggcctacca ggcgcgcggc atcgtcttca agcaggggta cggcctgacc gaggtcggcg
    43441 tgaactgctt ctcgatgtcg gtcgaggagt cggtcgcgaa gaaggggtcg atcgggaggc
    43501 cgctcatgat gaccgacgcg aagctcgtcg acgagtcggg cgccgaggtg acggccggcg
    43561 aggtggggga gctctgcctc cgcgggccgc acgtctcgag gggctactgg atgaaccccg
    43621 cggcgacggc cgccgccctc gacgacgagg gcttcttcca cacgggcgac ctcgcccgaa
    43681 aggacgccga cggcttcttc acgatcgcgg ggcgccggaa ggacatgctc atctccggcg
    43741 gcgtgaacgt ctacccggcc gagatcgagg gggagctcct cctccacccg accgtcatgg
    43801 acgccgcggt cgtcggcgtt cccgacccga cgtggggcga ggtcggcgtc gccttcgtcg
    43861 tcctccgccc gtgcgagagt gccacgaagg ccgagctcct cgccttcgcc gagtcgcgcc
    43921 tcgcccgcta caagctcccg aaggacgtcg tcttcgtcga ggcgctcccg cggacggcct
    43981 acgggaaggt cgtcaaaggg gagctccgcg acgcatacct cgcgggccgg ggcgccgcgt
    44041 gagctccacc gctctcctcg cgcaccaggt cgacggggag ggcgagccgc tcctcctcct
    44101 gaacggcggg atgatgagct tcacggcgtg ggacgcctac gtcccgcctc tcgcggcgaa
    44161 gcaccgggtc atccggtgcg acctgcgcgg gatgctccgc tcgccgggcg cccgtcccga
    44221 aggtttcccc ggccacgcgg aagacctcct cgcccttctc gaccacctcg gagtctcgag
    44281 cgcccacgtc gcggggacga gctacggcgc cttcgccggc ctccacctcg ccgcccttgc
    44341 cccggaccga gtcgcctccc tcgtcgcgat gaccgtcgcc gaccgcgtgt cggccgagat
    44401 gtgggccgag gcggaacgga tggccgtcgc ctgccgggag gcgctcgcgg ggggctcgcg
    44461 agagaaggtc tacgacctcg tctcggcctt cgccttctcg gaggaatggg ccgaggcgca
    44521 cgcggaggag atcgcggccc gccgcgccgc ggtttccttc ctgcccgact cctggttctc
    44581 cgggctggcc tcgctcctct cggcgctcga ggggctcgac gcgaggccgc tcctcccgcg
    44641 cgtgacctgc cctgtcctcg tcctcctcgc ggtgggagac cgcgcgatgc cgcgcccctg
    44701 gggcgagtcg ctcgtggcgg ccctgccgcg cggggagctc gccgtcgtcc ccggctgcgg
    44761 tcacggcctc gtcgtcgaga agccccgcga gacgctcgcc catctcctcg ccttcctcgc
    44821 ccggcacccg ctcccggtcg aggccccgac ccgccagccc gagcggaacg gaggaacgcc
    44881 gtgaagccga tctacatcgc cgcctaccag cagtcgaagt tcgggaagct cctcggcgcg
    44941 accgtgcccg agatcatccg gacggccgcc gaggagacgt gccgctccgt gaaggccgaa
    45001 gcgtccgccg tggacgtcgc cacgatcggc gccgtctgca acctgacgct caacgagcag
    45061 gggctcctct ccgggctcct cgcgatgacg cccggcctcg acggcaagcc gatcgaaagc
    45121 gtcgagaacg cctgcgccac gggcgggcag gcgatcctcg ccgtcgcgca gaagctgatg
    45181 ctcggcgacg gcgacgtcgg cctcgccgtc ggcttcgaga agatgcgcga cgccgacggg
    45241 aagatggacg gcaagctcgt cgggaaggtc ctcggcctct actcgcaccc cgacgagcgc
    45301 cccgggaaga cgttcgtctt cccgcacctc ttcgccgagg tgatgaaggc ctacatggac
    45361 gagtggggcg cgaccgagga ggacctcgcg agcgttgccg cgacggagta cgcgaacgcg
    45421 cgccacaacc cgtacgccca gatgcagaag aacgaggtga cgctcgagac ggtgatgagg
    45481 atcgagggat tcaaccggta cgtcgtcgag gggctccctc tgaagacgtt cgactcctcc
    45541 cagatctccg acggctgggc cgggatgctc ctcgccaccg aggaggggct cgcgcgcctc
    45601 ggcgtcgcga aggccgactg cgtgcgcctc gcgggctggg ggcaggcgac cgnnnnnnnn
    45661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcggcgt cgcgaaggcc gactgcgtgc
    45781 gcctcgcggg ctgggggcag gcgaccgacc cgctgaagaa ggaggggcgc gacgtcctcc
    45841 gcccgagggc ggcgaacgcc gcgatggccg cggcctaccg ccgctcggga ctctctccgg
    45901 ccgacgtggg cgtcgccgag gtccacgact gcttcagcgt catgggggcg atcggcgccg
    45961 aggtcatcgg caaggccgag ccggggaagg gggcgcgcta ctggcgcgac gggaaggccc
    46021 gcgtcgacgg ggagtgcgga atcaacacct ccggaggcct catcgccaag gggcacccga
    46081 tcggcgcgac cggcatcgcg atggtcggct ggtgcgcctg gcagctcctc gggaaggtgc
    46141 cgaaggagct ccaggtcgcg aacgcgcgcg cggcggcgac cttcaacatc ggcgggccgg
    46201 tctgcgcgtc ggtctgcacg gtactcaccc cggcctcctg acggagccgg gagggctgac
    46261 cccggcggcc ggacgcggcc gggagtgcgg gaggacccga tgggcgagat cgtccccgac
    46321 cgggaggggt tcctcgaggt cggcggtcac cggatccggt gggagttcgt cggcaagggc
    46381 gacaaggaga ccgtctgcct cctcaacggc ctcgcgatgc acacccgcgc ctggtacggg
    46441 gtcctcccgc gcctcctcga cgcgtacgac gtcgtcctct acgactacct cggccagggg
    46501 cagtcctcgg ctcccgacga accgtactcg atcccggagt tcgccgggta cctcccgcgg
    46561 gtgctcgacg ccctcgggat cgagaaggcc cacgcggtcg gcgtctcgta cggcggcttc
    46621 gtcgcggccg aggccgcgcg gctcttcccc gagcggctcc acacgctgac gctctcgggg
    46681 atcctcctct cgcacgagga gctcttctcg atgtaccagg agatctccct ccgcttctac
    46741 cgcggcgggc cggaggtctt cgagctctac acgcactaca tgtacgagaa gatcttcggc
    46801 gaggcgttcg tctccgccat gaaggagagc ctcccggcgc tccgcggccg gttccacgac
    46861 cggtaccgga gccagacgca cgcgctcatc cgcctcacgc gcgcgcagga cccgttcttc
    46921 gcggcgctcg acgggaacct cgcgggctac gcggcattcc cggaaccggt cctcgtcgtg
    46981 ccgggcgccg aggaccggac gatcccgccg tgggtccagc ggaagctcct ctccgtcttc
    47041 cgcgacgcgc gctgggagcc gatcgagggc tccggccacg tcgtctacct cgagaagccc
    47101 gacgccttct tcggcgtcct ccggcggttc ctctcggcga agtcgacgtc gtactgacgc
    47161 gcgaggtcga cggctttccg aaggtcagag gtccccgatc gcggcgacga ggcgcgtcca
    47221 gagcgcgccg ccgccgaggc cgttcgtcag gacgacgatc ccggtcccac gcgacggcga
    47281 gagctggccg tagcaccgga agccggtctg gttcgcgccg ctgtggtaga tcacgtcgcc
    47341 gccgggggtc tcgttgatcc cccagccgag cccccagaag accgctccgc cgcgcgctcg
    47401 cccggggcgc tcgatcgggt cgcgcgcgtc ggcgcggacg gcgcggcgga gcatcgcgtc
    47461 gaccccggcg cgcgagagcg ccttcggccc gaaggcctcc gggtcgagga gagccgtcag
    47521 gacgcgggcg tagtcggggg cggtcgtcac gagggtgtag gccgcgttcg cgtggcggta
    47581 gcgcgcccgc gccttcggct tcccgtcttc gtcgtgcccg gtcgcgagcg cggcgtcgag
    47641 ctcgggggcc caggcgaacg acgcccgctc cattccgagc ggggcgaaga gcgcgtcctt
    47701 cgcgtacgcc tcgagcggcg cgcccgttac cttctcgacg gcgcgctgga ggaggaagaa
    47761 cgcctcgccc gagtagccga agcgggagcc cggcgggaag aggacgggga gcggaccgtc
    47821 gcgctccccg cctcccgggc gccagttcgg gaggcccgag gtgtgggaaa ggaccatccg
    47881 cggcgtgatc cgcgtcagct ccggctggcc ggggacggcc gggaggtcga cgacctcggc
    47941 gagcggccgg tcgaggtcga gctttccgtc ctcgacgagc ttcagcgcga cgtacgcgaa
    48001 ggccggcttc gtcatcgagg cgacctcgaa gagcgtcgtc gtcgtcgcgg gagcgccggt
    48061 cttcgcgtcg gcgacgcccc aggccttcga gaaggccacc gtccggtcct tcacgagcgc
    48121 gaccgagacc gcgggaacgt ggtgctcccg catcaggatc ggaacgagcc gctccacgac
    48181 cgcgaccgtc gcctcccacg acggggcgcg gagccacgca gcggggtcga tcctctcgag
    48241 cgcgagggtc gcaccgcgcc ggacgtcctc gtcgcggtcc cagagggcgt cgcggagggc
    48301 gggaagcgcg ggggaggcgc cgcggccggc ggcgccgagg gcgctcgcgg cggtccagcg
    48361 gacgaccgcc ttcgggttgg agagcgcctg cgcgagcgcg gggacggcct cggtcgccct
    48421 cggcccgagc tttccgagcg cgacggcggc tccgacgcaa acctcctcgc gggcgtcgcc
    48481 gagcgcgcgg acgagcgccg gtacgctcgc cgcgccgatc cgcgcgaggg cgtcggccgc
    48541 cgcgcccgcg acgaaggggt cggcgtcggc gagggccgcg acgagcggct cgacggccgc
    48601 ggcgccgccc gcggcgaggg aggcggcggc gcgctcgcgc gccccggggt cggggtcggc
    48661 gagccccgcc ggcgcctgcg cggcgacggg acgggcgagg agcgcgagaa ggagcgggac
    48721 gaggaggcgc cggctcatcg cgcgcccccg gggaggaccc gcgcgaaggg cgcggcgtcg
    48781 tcgaatgcgt cgtcgaagga gaagaggatc gggagccctc ccgtgtggag gaagacgacc
    48841 gggaccgcgg ggtcgaggag gccggcgcgg gcgaggccga agaggcccgc catcgccttg
    48901 ccggtgtaga cggggtcgag gaggagccct tcgagccgcg cgctctcgag gatcgcctct
    48961 cgccccgtct ccgtcgcgac gccgtactcg ccgcggagcg cgtcgtgcgc ctcgacctcg
    49021 gaggggccga gaacgggctc gaggccgagg aggcgcgcgc tgccggccgc gagctcggcc
    49081 gcccgcgcgg cgatcgggcc ggccgcgcgc gagacgctga tcccgatgac acgcgcggcc
    49141 gggaggaaga gcctcgcgcc gagcgcgagg ccggcgagcg tcccgcacga cccgacggcc
    49201 gcgacgagct ggaccttccc cgggccgagc tgcgcggcga gctcgcgcat cgccgcgacg
    49261 tagccgagcg cgccgtgcgg cgtgcttccc ccgagcggga cctggaacgg ccgccgcccc
    49321 gcggcgcgca gctccgcggc ccacgactcc atcgccgccg cgagggagtc gttcgcgtcg
    49381 tcgtcgacga ggtagcgcgt ctcggcgccg tagagcgcgc cgaggaggag gttccccttc
    49441 cg
//
LOCUS       DAOONL010000019        44950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_589_length_44950_cov_22.664952, whole genome shotgun sequence.
ACCESSION   DAOONL010000019 DAOONL010000000
VERSION     DAOONL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 44950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44950
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_44950_cov_22.664952"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(671..1342)
                     /locus_tag="PLP50_04945"
     CDS             complement(671..1342)
                     /locus_tag="PLP50_04945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA50932.1"
                     /translation="MGFERRIRLLFALGGVVFGFALGFTAGFYSAGALFKKLMNGIQG
                     APTLAAGGGGVARGDALPVPRMNANAATTSLLEFNAVLEDAQGHSVPLSTFRGKVVFI
                     NVWATYCQPCIAELPALETLYASFEGHRDFAFVSISRDSATTLRGFLHTRTLRFPVYR
                     FAPGSDISGLGTAVPVTLIVDHTGAVVSRIFGAANWADRKFIEDLRIRIDQAAAPSAS
                     ATPSG"
     gene            1651..2004
                     /locus_tag="PLP50_04950"
     CDS             1651..2004
                     /locus_tag="PLP50_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50933.1"
                     /translation="MLEVVGDATVLSYQLARLTDEPQRFAEVLTSEDGDIVISPLDKQ
                     PLAGGGVEHPGDRTAVGKLNLDEAQPLLKEYTQPAGIGSVSIVCLNEARLLQIKLNRC
                     DLSSCGIADVNEGRG"
     gene            complement(2775..4265)
                     /locus_tag="PLP50_04955"
     CDS             complement(2775..4265)
                     /locus_tag="PLP50_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50934.1"
                     /translation="MKFRPAVVGPLLAACLATPILGQGDHPNRAAGISAQTLYSSSGS
                     DDINLFNGNLHDLIPIGEPVAVGPLLSIAPALVYNSQAEKVWRVCKENEEHPTGDRAI
                     GPGWRLHFGRLGPVETRIPRGSIDYWQPSTPEAPLVDDAVYGMGSTRWAYEDTDGGIH
                     EFYDRLVESSCSISDPACEPTHPPKCFGPTEHGAGHCFAYTHDGSFLRLDPIDPEHGG
                     HPTVYFPDGTFRVMGFRGGRYPILGTSLPTDLDHAETVPAYHTTLAGDLFGNQYSVQY
                     YGDGTQGSPANPLLEHSIPENQWDAIPFRVFPSTAGSDAGVSRLRALRALRGVPRGHL
                     DPLVREGFVVAVFEGAEGVSGVVDEDALDGERHRSARPEALPHEACVGGADDPGLLEA
                     ELGRHGPFHGPQEEGVGGLLRSEGLVGVEDLDAVGAAYELVVASLVGVLGSAPAADVA
                     DEDGPEVRLSLDVPDETLESVQLLDAFAGGAVAVDADDFVPRFRGV"
     gene            complement(4426..4764)
                     /locus_tag="PLP50_04960"
     CDS             complement(4426..4764)
                     /locus_tag="PLP50_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50935.1"
                     /translation="MHFRDDPPRARGAPKSILIAGRSFAFACGLSGEELHALQEEVRA
                     RGGRIDVDLTPETDVLVVGSLPGIGRLAEKGREVILRGELYRDRIGLLEFVTEEALRA
                     TLSASPGGEA"
     gene            complement(5442..5516)
                     /locus_tag="PLP50_04965"
     tRNA            complement(5442..5516)
                     /locus_tag="PLP50_04965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5482..5484),aa:Asn,seq:gtt)
     gene            5640..6581
                     /gene="gluQRS"
                     /locus_tag="PLP50_04970"
     CDS             5640..6581
                     /gene="gluQRS"
                     /locus_tag="PLP50_04970"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HPA50936.1"
                     /translation="MTDPRPRGRFAPSPTGPLHLGSLVAALGSWLFARRAGAEWIVRM
                     EDLDGPRVVPGAADEILRALERYGLTWDGEVAVQSRRTVFYEEALARLRAAGHAYDCG
                     CTRAEVARAAGAPDAADPVDAAGAVYAGTCRAGLPAGRAPRAVRFRVPEGIVAFGDRV
                     HGRIEEDVARAVGDFVVKRADGPFAYQLAVVVDDAAQGVTEVVRGGDLLSSTARQIAL
                     QRALGLATPSYAHLPLVLGADGKKLGKRDGALPLETLDERRVAESLALALRLLGQEVA
                     GEAPGEMLAAAARSFDVTRVPTGPLRVAADGGIVSGA"
     gene            complement(6568..7461)
                     /locus_tag="PLP50_04975"
     CDS             complement(6568..7461)
                     /locus_tag="PLP50_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPA50937.1"
                     /translation="MPRRAAARRNGTPAASGRRRAAAPLRTVLVVGGAGRMGRLLARA
                     FRAAGHALAVHDPGRRLAGFPSVPLATAAEADAVVVSVSLEATPAVLAELLALSPPGL
                     VLDVASVKGPLLPLYAKAARRGLRVASAHPMFGPGVRSLAGQNLIVCNAGHEAAARDV
                     ARLFSGHGLKLATMPIGAHDPWIATTLGLAHLLSLLAGSVLARGGVSATGLDGLASTS
                     FRNLVLLAAPILRQSPDLTLLIQARNPATPALFDLLAEELATWRALVARNDLAAFERK
                     VGEAASVLTSPGPGRRRPTRR"
     gene            complement(7466..8701)
                     /gene="tyrS"
                     /locus_tag="PLP50_04980"
     CDS             complement(7466..8701)
                     /gene="tyrS"
                     /locus_tag="PLP50_04980"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPA50938.1"
                     /translation="MPNFAPVPDQLELLLKGVETCVQKDELAAKLEASRKSGKPLRVK
                     VGFDPSAPDLHLGHTVVFRKMRHFQELGHEVVFLIGDFTGLIGDPTGKKATRPQLTKD
                     EVLANAETYKTQVFKILDPETTVVDFNSRWLGALGADGLIRLAGRYTVAQLLERDDFS
                     KRFKAGQPISVHELLYPLCQGYDSVFLEADVELGGTDQLFNLLVGRELMRSYGLAPQC
                     VMTTPLLVGLDGVEKMSKSLGNHVGVSDPPDEIFGKVMSVSDDMMWTWWTLLTDLFPH
                     DIDALKADVASGAKHPKRVKMDLARQLVADFHGADAAAAAEAEFERRFAKAEGPVKAE
                     TVSLPDPAPADLASLLVAVGLAPSKNVARQKVKEGAVATSGDGVAFTKADNPAEPFAL
                     EPGAARYVRLGKRFLRVAR"
     gene            8772..9992
                     /locus_tag="PLP50_04985"
     CDS             8772..9992
                     /locus_tag="PLP50_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPA50939.1"
                     /translation="METEDLNRLIRSLNAAEEGPAGEEPLVEHVRELEAAEAVGLRTG
                     APIADGELSPRLAALLTETVRRGGTDLLLVPGAWPVLRLHGSLEPVSAAAGEPLGPSE
                     AFDLLSPSLSAERRRRYGAEGAVDLSVRLGALGRFRVNLHRTRRGTAAAIRVLPKRIP
                     PLAELGLPESLYDLTKAARGLLLVTGATGSGKTTTLAALVDRINRTQKRHVVTIEDPV
                     EYEHAHGASIVEQVEVGADAPTFAAALVAALRQDPDVILVGEMRDLETTRAALTAAET
                     GHLVLATLHTNDVPQTVYRITDIFPAEQQATVRHQLSLALSAVLCQQLVPRADGKGRV
                     VAAELMLATDGVRAHVRKGTFHQLHTELTLGRRLGMVTMEESLARLVRSGAITEAEAR
                     LRAAHPDDLAAHLR"
     gene            complement(9999..11465)
                     /locus_tag="PLP50_04990"
     CDS             complement(9999..11465)
                     /locus_tag="PLP50_04990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA50940.1"
                     /translation="MNVRPNLVGGYTILERLGRGGLSGVFRALRDADGLEVALKITSL
                     ADLDPEFRPQERFEREAELLGRLSHPALPKFHDWGVTLDGYGWLAMELVRGAPLSQFA
                     LRPAWELIPILIPIAESLAAVARGGIVHRDVAPDNVLVAMEDGRFAPKLIDFGVAKDL
                     FASAAAGGLTQHGAFLGKLSYAPPEQLLGLPKGQTLDFRADVYSFGLTVWELLAGRQA
                     VAATTLPEIVNAHLTRSWPRLDLPPARGGPAPKLLALVERMTARRREDRPSSWEEIVA
                     ALWQALDEVRPVHRELEQKKAELAPFDHVPPPEPRQDGAGADAPFPEAATPPAEAPVE
                     DVPVAPAPALAPAARLLTRELLLGRVVLIFGIAALVAALAFALHVVVSAPEPAAEAEG
                     LRPAIAGRDTGPPSAAGAPAGRLLVALVPGGVLEEVVDEAGRRVAGPRPLPASLPLPP
                     GAYRARLAHPESGCAMTVTFDIRPGGTTRVLESCLGSE"
     gene            complement(11526..12626)
                     /locus_tag="PLP50_04995"
     CDS             complement(11526..12626)
                     /locus_tag="PLP50_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50941.1"
                     /translation="MPIVVRFLSGSLTDRDFSFPGEVVRVGDAEGVDLRVDPAAPGDG
                     GARGRVIEISAEGSGFRIRSAGNRDLSAQGEFPLDHPVPDGGEVRFGAWGPVFTVRRV
                     SAELPFTAPQPFPVAEPSRADDTASRRRAEDTGSRRRSVLTASGEKPVGPKTVMVMIQ
                     DALSKAREQGDSGFVEKGTVFVREVVADTITSATRSLKIGLLLTGAALVILAFALGYN
                     VWRTRQTVAHVTRAADESVQGVRKELGTRVEEMRQERDALAAESAEVARKLGELEKTA
                     GASEKAVSDLRRRLGDADARRKDLEGRMQRALDALAADKEALQRQLEQMERERAADRA
                     LQQAEIERLRQETTAPVPLPVEPVPTAPPAPR"
     gene            complement(12694..13314)
                     /gene="udk"
                     /locus_tag="PLP50_05000"
     CDS             complement(12694..13314)
                     /gene="udk"
                     /locus_tag="PLP50_05000"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPA50942.1"
                     /translation="MTAAPLAIGVAGGTGSGKTTVANAIRNRVGEDRIAILSHDWYYR
                     DWVDLPKDVLDHKNFDHPDSLESDLLVRHLKALKSGATVEVPVYDFKTHRRAPETRRI
                     EPRKVILVDGILIYAEPALRTLFDVRIFVDTDADVRLIRRIRRDIAERGRTLDSVVTQ
                     YESTVRPMHLEFVEPSKRYADLIIPEGGENAVALEFLFARLEKILG"
     gene            complement(13340..14278)
                     /locus_tag="PLP50_05005"
     CDS             complement(13340..14278)
                     /locus_tag="PLP50_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPA50943.1"
                     /translation="MNQKKTLSAALIAILIAVSGIALAGPQARIVGTVTDAKGGPIVG
                     ATVTITTPAFGKFKVVLTTDKDGKWGTILNDSTIAYDYLVEAKGYIPIKQNKKVGIGS
                     EGTLDVQLLTADQAVEKGLVKEVVDPYTLAFNGAVEKFQAGDLDGAIEGAKKATELGP
                     DKAGAWDMATKVAAAKKDWDLVIQWGEKALSIEPDNGSLYGALMEAYKAKGDKAKALE
                     YEKKFIAANPDKPEILYNQAVDLYNKGDFKGADPILRKVIEGAPEYANAHFLLGMSCV
                     NLNKIPDMKKHLAEYIRLDPKGKEVATAKEMLEAFK"
     gene            14431..15096
                     /locus_tag="PLP50_05010"
     CDS             14431..15096
                     /locus_tag="PLP50_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50944.1"
                     /translation="MPKGLFRWLRGGGPPAGDDPRGADRLAAAPERRPGVTVHAAGSL
                     AELGADAGRGGVSRLGPFAPLVTDGGCAWAGVALLGETAGDVFFPAAGATGLSSGVAA
                     LLDGVRTEEEDAPARIARGSLAGSAVPGVTGARLLFRGGGRAVLRLGPPPAARWAVEG
                     LRSVAVLSGKLTLVDGEEARDVRAGEVAFVAEPAATLHVQAGNDAALAVGFGRPELSV
                     RLG"
     gene            complement(15102..15635)
                     /locus_tag="PLP50_05015"
     CDS             complement(15102..15635)
                     /locus_tag="PLP50_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPA50945.1"
                     /translation="MYARPLYCSQCGQRLEERSVGAGDGKVVPVCPACGAVHWIDPKV
                     AAGCLVVRDGRVLLVRRGIEPGYGLWVFPGGHVDRGETLEEAALRETREECGALARIE
                     GLLGVYSYPGRPVIVAAYRASLLSESPEPYAADETLEVGWFAPEEVDRLDLAFRSSAD
                     ALSALLGRRVRAPRHEG"
     gene            15803..16471
                     /locus_tag="PLP50_05020"
     CDS             15803..16471
                     /locus_tag="PLP50_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50946.1"
                     /translation="MSRHWTQDEIRWLNANAERMDLPTVSRTLGVPLVEVERKLAELR
                     ADAASSQKKPPATHREAARELTHARKLFEKGMEHLHRREMSAAAACFSELLELQPDER
                     ELVDRARTFLAVARNGKSTKEKPLRDPAEIYHAAVFEKNRGNCATAIELVRSADAQAD
                     PDGRLAYLAACCYALHGRPDEALESLRAAIAASDQNRIQARLEADLAPLRSQPRFEAL
                     VGRG"
     gene            16474..17892
                     /gene="glmU"
                     /locus_tag="PLP50_05025"
     CDS             16474..17892
                     /gene="glmU"
                     /locus_tag="PLP50_05025"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPA50947.1"
                     /translation="MPAATAVVLLAAGRGVRMKSARPKVLHEAAGRPLVDHVVGTARA
                     FLASRPGSRLVVVAGAGREELIPHLARTAPEALVAVQDPPRGTGDAVRVALPALGDAV
                     RVVVLAGDVPLLTAASLARLDEALDAGAGIAVLTALLADAGAYGRVVRDAAGRFERIV
                     EAKDASPEVRALGEINSGIYAFDRALLERELPRLTDDNAQREYYLTDVLGYAVGDGAA
                     VAALPVGDPAEVLGVNSRLELAEVDALLRRRAARAAMAAGATLIRPETITLDDGVTFG
                     PDVVVEPFATITGETSIGAGTRIGQGCIVGSSTIGANVSVRPYSVLEKAVVGDGAVVG
                     PFARLREGTDLGEGVHVGNFVETKKARLEKGAKANHLTYLGDCRVGERTNVGAGVITC
                     NYDGYGKHFTDIGAGVFIGSDVQLVAPVAVGDGALVGAGSTITKDVPAGALATSRVPQ
                     KTVEGGGTRYRERKTAAKDRKG"
     gene            17897..19741
                     /gene="glmS"
                     /locus_tag="PLP50_05030"
     CDS             17897..19741
                     /gene="glmS"
                     /locus_tag="PLP50_05030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPA50948.1"
                     /translation="MCGIVGYVGPREATPILIDGLKRLEYRGYDSAGIAVLGADGLVR
                     VTRSEGKLGNLTARIESEPPAGRTGIGHTRWATHGRPSEENAHPHRDASGRVVVIHNG
                     IIENFLPLKRELLARGVAFTSETDSEVIAQAIGAARADAPAKPFGEVVRTVLSRMTGM
                     YAVVVLSADEPEVLYVAKNGPPIVLGLGADENLVASDATALLPHTRDLVFLEDGDFVR
                     LTATSVEVAGLDGAPRSRPARRVPWDAVAAEKGGYPHFMAKEIAEQPTVVVETVGHKL
                     SLETGRFSEDAMGVSPRLLSEVDRVVIVACGTSWHAGLVGKFLLEEVARVPVEVDYSS
                     EFRYRRNLVGPRTLVLGISQSGETADTVAALTDAKRAGAPTVGVVNVPGSAIARLVDG
                     VLATHAGPEIGVASTKAFTTQLVALALLAYYARAARDLGRLEPGDPFLDGLAHLPVAL
                     QQALALEPAIEKLSRTLAHARDVLFLGRGAHYPVALEGALKLKEISYIHAEGYPAGEM
                     KHGPIALIDGELPVVALAPRDTLYDKVASNLREVKAREGKLVALVSGGDVDVATFADE
                     VLEVPLVHPALQPIVSVVPLQLLAYHVARRRGCDVDQPRNLAKSVTVE"
     gene            19812..22112
                     /locus_tag="PLP50_05035"
     CDS             19812..22112
                     /locus_tag="PLP50_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPA50949.1"
                     /translation="MNDREKALLEGLNPEQAAAVSAGPGPLLVLAGAGSGKTRVLTRR
                     MAWLMAHGTPESAIVALTFTNKAAGEMKERVAHLLGSRPPRSFVGTFHAYGVRLLRRF
                     AVEAGLTPGFVVFDADDQLAVFRQALKAAGISDKALTPKAVASKISRARNAGWSRDEY
                     PRRFGDFVGTRLADLHTAYEKELKKANAVDFDDLLVRTRGLLERSPEVLALLRRSVTQ
                     LLVDEYQDTNGAQAGIVRLLAGEARNVFAVGDEDQSIYRWRGADVSNILDFEQDFPGA
                     RTIRLERNYRSTAPILDAAGAVVAENRRRLGKNLKATKPGGEPVTLLVLPEERDEARE
                     VVERISRVRSVRPTAEVAVLFRTNAQSRPFEDELVRNRMPYLLVGGTRFYERAEVKDA
                     LAYLRLLRNPDDDVSFRRVVNVPARGIGAATLEGLSVAASARTSSLFAVLSALPDGLT
                     ERAKKALVEFRDLVSSLREEFAAEEAGSAAAVAAVLTNTGLLALYEGSDDPQDQARRE
                     NLDELLAAAREHERSGAEGDEADRSLAAFLDAVTLRSDADDVDERKGIVLMTLHAAKG
                     LEFDEVFLAGMEDGSLPHFSSRQDEDELEEERRLAYVGMTRAKEKLTLSRVLRRMVHG
                     EWTSREASPFLDAIPEQTLAVVDRTFGPASADGFRGAGRGGDLFPDYEGESQERWTPQ
                     RPAARPVARPAARPARAAIPTMRRTPPPPTASGFRRGGRVRHPEYGIGVVLLVEGSGD
                     GEKLTVYFDRAGRKKFVARYSNLLPA"
     gene            complement(22146..22658)
                     /locus_tag="PLP50_05040"
     CDS             complement(22146..22658)
                     /locus_tag="PLP50_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPA50950.1"
                     /translation="MSLPHVLLGLLSRAPASGWDLKSRMSRDLSLGWDAELPQIYPAL
                     RRLLRGGFVVMKRRRSEKGPPRRVYRITPAGRRELHEWLAEPLALPRPRDAALARIAF
                     LERRPREARAARLLEYRALVVAALKRASPGSTAARRRWRALLEAERAWVDAERALLEA
                     AAAEEPAAPR"
     gene            23266..24129
                     /locus_tag="PLP50_05045"
     CDS             23266..24129
                     /locus_tag="PLP50_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA50951.1"
                     /translation="MSNVEASPLVLAHSPDSDDAFMFFGLRAEAVDTEGLAFRHELAD
                     IETLNGRALRGELAITAVSFHAYAYLARTYVLLPHGASFGDGYGPCVIAREADDAPAT
                     LESLAGKRVAIPGALTTAALALRMRVPLAVPVLLPFDRVGDAVAAREVEAGVVIHEGQ
                     LTWKEEGFRLLGDLGALWTKETGLPLPLGGNAVRRDLGDETIGKISRALGRSIEYSLA
                     NREAALTDAMRFARGLPREKADRFVGMYVNDWTRSYGERGREAVRLLLDRAFEAGVVP
                     SRVVPEWVAEG"
     gene            24126..24674
                     /locus_tag="PLP50_05050"
     CDS             24126..24674
                     /locus_tag="PLP50_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HPA50952.1"
                     /translation="MSLERFTRGSALRAALLLVVGALLGALVFFAGAAGTVLRESPSR
                     HAGGAVNRALLDLLDLSTYGVAAVAFVLVLLLDRVEPLGKVQKGLALRLLAVLAAAAF
                     ASHEVITPQMMALRDRLPTLIDLLPAGDPGRRAWGRLHAFSSGILVVRIATAAFLFVL
                     LGRRRRRTIATTEAPAGVPPAA"
     gene            complement(24697..26004)
                     /locus_tag="PLP50_05055"
     CDS             complement(24697..26004)
                     /locus_tag="PLP50_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA50953.1"
                     /translation="MRRSILLAAFALAVLASPAAPSRAQGIAFSEGRPFAEALRRARA
                     EKKPILLDAYAVWCGPCKQLDRLTFADPKVGEWARKNVVAVKLDAEKGEGRRISQRYA
                     VRAFPTVLFIDPAGNEIDRLSGVFGPEEFLRAADLIVAGKAPLATAVAQLKEKWDPAL
                     AGGLAAQLAQRNDLGRLRPIALRAVEEDPSLAEEGAREAFLYLVSLEHGAGALSAETL
                     DLIESLAPRLAADPRAALLYLAAAEAQARAGNGAAARASALAGLKLIPEDAPLAVDLH
                     VALAAAERAAGKPEAALAAARRAVALGEKAAGGAWGRASRLLALAETLAFARQGEEAK
                     KTLDVALASVSNDPALLVRASALLVRLKDHSGAVARARSAVEASQGGDARAQAALGEA
                     LAASGDVKGAVAAFGRAVELEPENRDARRRLDALRSRTAKKPA"
     gene            complement(26109..26942)
                     /gene="truA"
                     /locus_tag="PLP50_05060"
     CDS             complement(26109..26942)
                     /gene="truA"
                     /locus_tag="PLP50_05060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPA50954.1"
                     /translation="MIPSHAPSKTSAPAGAVLRATIEYDGSRYRGWQAQVNARTVQGV
                     LLAAARELWGPGVRVHGAGRTDAGVHALGQVASVHLPPRARVDAAASLRRWNELLPFD
                     VNVLALSPAPPSFHARHDAVRRVYRYRISTRRTAFGKPFVYWSKDALDVAAMDRAARL
                     LAGRHDFAGFCENPEGHDSTIVVVEEARVAAPAEADGLVLVRIAASHFLWKMVRRVVG
                     VLLEVGAGRMTEAQVARLLVERSVEVARLTVPPSGLFLESVGYPGEAAGEPPHPLRPA
                     F"
     gene            26997..27437
                     /locus_tag="PLP50_05065"
     CDS             26997..27437
                     /locus_tag="PLP50_05065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50955.1"
                     /translation="MRRRWTIHLSVEADFPSVRLLAAACHGVLAEWGLPTERRSLLEL
                     AVVEAATNVVRHAYRGRGGRVEVELTREGRTISLSVVDTGIPFDPTRVPPPRDLDPDD
                     PSTWPEGGMGLPIIRAACDELRYVSDGARNELTLRLAVDEPEET"
     gene            27434..28600
                     /locus_tag="PLP50_05070"
     CDS             27434..28600
                     /locus_tag="PLP50_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50956.1"
                     /translation="MSVPDRPFTAAAVPAVERRCRETLAALVSRLGEVTGDGLAGSAL
                     GGALGRGEAPTTVDAEGAFVTPVPFELLVVLEATPGRAASLAPRLRRELATTARARSA
                     DVRLATAARSALAQLPPTISNLETAAAGRVVAGPAALLSPLAPLAGAPPAAAEALALL
                     VRRGAALHLAERGLSGRAPSRAALLAGQAAVRAVDLALGAALLVSAGRFRPTDAARDA
                     ELRLLAAPAGDGPAPSGFHLRMSRTRFRDVVDRHRAAVEAAREVDLPDTLGEARAQAG
                     RATDRFVEVLRLLEEERLSRPLPSWTDYVRALAERHGGSPGPGLFETRREEELPSRRS
                     VRAWPVAERLAPALATLLDWDPGDLDVVPLLLDLPADATKEALGARLAAWAASA"
     gene            28641..30392
                     /locus_tag="PLP50_05075"
     CDS             28641..30392
                     /locus_tag="PLP50_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA50957.1"
                     /translation="MTSVTEMADDPGASGRLLFVLALLAGLLLRLVQLGSPDLLGPEE
                     GPWAVGARNIVEGGLAQLLALSSTPLGEPTGIPIFFPALLAAAVKTVGAYEWAIRLPS
                     VLFGLVAAFVLERIVRRGYGQPAGHIAGAFAALASPLVLSSRTATVEPTLTLLGLGGI
                     IFGLRAFEEDSPGEALPSGVLFGLGFLAKGYAVGLFVIPLLLALLFRPRLFTLGRTKR
                     TLALLAGSFFLTAGSHLLLLALVHPASLAPHLSRAFGSAPDALRQLSDVALVSADLRM
                     IVKSLFVLLPLVGVGIAYLARPLGEEEVVSGATGGERRLSHMALWCAYGVELVVLVAV
                     AGSLELSSTPVLPALSALAGLGGAALLTRPRSPRRVRFETIATVVSGAIVLVLAVLLV
                     WTAREPLFNGGRAAPFTAAGALAAIALSTAGAAWLVSGDAARRFGRRTGLAFLSVLLV
                     SEGLAAARAIRHDLLAHRTGVRALADQIAPELLPLSPRAVTFRAPEPAAIAFRLFRTG
                     TTWADRPTVAQIAAEARGGTTVVWAWREGTTDPVAAPAPLVRAWLIVNTREVTAEVDA
                     RAGRTTGLRAFVARPPS"
     gene            30404..31759
                     /locus_tag="PLP50_05080"
     CDS             30404..31759
                     /locus_tag="PLP50_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPA50958.1"
                     /translation="MTTVLLADRDGRALGPLADKTVPALLPLRGAPILERALEALVSA
                     GVRSALVVVGPRGSEIEKRFGKGIRWGIALEYVRRSEDETTGAILRRLEHRLDGETLV
                     LRGDAAIEGGYGEFLRRSAQSESPVVAAVSGERLLGMWRVRPEALKKLEIPREPADES
                     WTREKGHAPLDVGLDLAPLDSLSRWSALDRGDGAVALSPRAAVSKAARLSGGSTVAEE
                     AAVLGKATLDGVSVLPRSVIPDGVSLRGVAVSQNLVVDPVAGAASLLTDLLPPAGPAR
                     GAGIGSRLAGLVLFLLSLPLWPIAYAWAFVANAGRPTRPWAFAGNGAEPGTRRTVKSF
                     RFETAIPVFRDLPLLLALLGGSLALAGVAPLAPEEEETAARDAWVLARREAPVGLLAR
                     SRMAVPASAPGEVARVVDAFDARRGCRGLVPLGLSTFLSARAWTAPKGWNPDELPESQ
                     G"
     gene            31780..32226
                     /locus_tag="PLP50_05085"
     CDS             31780..32226
                     /locus_tag="PLP50_05085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPA50959.1"
                     /translation="MSDEHRYTASAAWDPAERSGALESGSRLLAVPFGGAPSLGGRDD
                     RTNPEELLLGAVLTCFVQTWAIFLAKLRLPLDSPRVDGALDLEADPAGGYRVRRIDLV
                     AHVPAALLAEREADVAKSLALAEKYCIVSKAVKGEGREVHVTTRPA"
     gene            complement(32508..34109)
                     /gene="nadB"
                     /locus_tag="PLP50_05090"
     CDS             complement(32508..34109)
                     /gene="nadB"
                     /locus_tag="PLP50_05090"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPA50960.1"
                     /translation="MSRVAGRTRVPALVIGTGIAGLTAALALAKRGIRVLLLTKAAEP
                     DDCNTRHAQGGIIYFGKRDSPTSLVRDVLAAGAGLCNAEAVRFLAQEGPRVVEEVLIG
                     DAGVPFTRTRRGDLDFTREGAHSVARIVHSADATGKAIELALLARVKRERNVKVWTNA
                     TAIDLITARHHSTDVQQRYALQDPCLGAYVLDEEGAVHTVLADFTVLATGGVGRLFLH
                     TTNTRHAIGDGLTMAARAGAAVLNLEYVQFHPTTLYHPDAERFLISEALRGEGAVLVN
                     RSGQAFMPRYDRERKDLAPRDVVTRAIVEEMTTRGEPCVYLDLAHHYEGKGRTAIRDR
                     FPTIAATCEKFGIDIATEPIPVVPAAHYFCGGVLADLEGKTTIRNLYAVGEVSCTGLH
                     GANRLASTSLLEGLVWGAHAARSIADRIEKGHTFPPSTFRAIPDWKAPGNARNEDPAL
                     IQQDWTTIRNTMWNYVGIVRTGERLGRAVADIRDLEKRLSRFYHETKLSREIVDLFHG
                     VHASHLIAQAALKNPVSRGCHYRRN"
     gene            complement(34106..35008)
                     /gene="nadC"
                     /locus_tag="PLP50_05095"
     CDS             complement(34106..35008)
                     /gene="nadC"
                     /locus_tag="PLP50_05095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPA50961.1"
                     /translation="MKTDRRLVEALDDLLVRAFAEDGEDVTTLAVFGPKARVEADMVC
                     RDAAVVAGVAFLPRVFAFLHPPSRVELRVEDGTKVAPGTVLAHVEGPAISVLAAERTA
                     LNLVQRLTGVATATRAYVDALAGTKAKLLDTRKTTPGLRALEKYAVRCGGGTNHRMGL
                     SDAFLVKDNHADGCGSLWDATRRAADFRAMNRSLRRCLLEAEARTRDEVHQALEAGAE
                     RVLLDNMTIAAMREAVRDVKLWNEATGATVTTEASGGMTVEKARKAARAGVDFVSVGA
                     ITHSVRAADVALDVKVAAARRGRR"
     gene            35075..35740
                     /locus_tag="PLP50_05100"
     CDS             35075..35740
                     /locus_tag="PLP50_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA50962.1"
                     /translation="MEDSMMAGPVIPERYRAALGDDDPFLAMFEAPQALRKLLKGLTE
                     KQLSRRPAPGKWSIKEIVAHLADGEVILGSRYRLVAAHERPAIAAYDQDLFVEKLGAD
                     AASTEELVADFEMARAVNLGLLVRLPDEAFARVGLHSERGEESLGTMVALYAGHDRIH
                     LDQIETIRTGLFPAKKKARKAAAKAAPKRAAGKAATPKRSAPKPGAKAPVKKAGRKAA
                     KRS"
     gene            complement(35750..36880)
                     /locus_tag="PLP50_05105"
     CDS             complement(35750..36880)
                     /locus_tag="PLP50_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPA50963.1"
                     /translation="MTAPPFDVLVVGASLAGSAAALVLARSGANVAIVDRAPFPRPKA
                     CGDLLSPDGVSALSRLGLERAVRAAGAATVRRFVLVRPDGRRLAGRLPAPALSLSRER
                     LDVLVLDGARAAGAAAFLGEAVTAIEGSLDAGFTVRTGARTLSARAVLGAWGRYSPLD
                     GRLGRPFFGAPAPLFGFKTFLAGDAGRRLDDRVALHLFRGGYLGLSLVEEGRVSLGAL
                     ATPAVAKEAHHDLERLLARLRAESPALAADLEGLAPEPGPALVSEPVHLGARQAVLGE
                     ILLAGDAAGVVDPYTGAGMALALRTGESAGTLLARHAAGEVPSPALGAEWGRRWRDAT
                     GRVYFFSRLFRPVFLGGAVTRLVVPVAGPLAGLAARLTRGPA"
     gene            complement(36877..37560)
                     /locus_tag="PLP50_05110"
     CDS             complement(36877..37560)
                     /locus_tag="PLP50_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA50964.1"
                     /translation="MSLLVPPRAPSDELLDGDVDPAEAVASLGDIERIHRLFGGRRTL
                     RRHLVPLLLAAPRPDPVVLDLGAGNGHVGRALSREMARHGRGLVTIDLDRKLLHARLS
                     AKGRSLAADALRLPLADDSVDVVASTLFLHHLDAHGVATLLRESARVARVAVAAFDLV
                     RHRAPLAVHALLSGFAYRSPVTRHDGRASVLQAWTKAELREIVAGALPEARVAAAGPF
                     VQGLVWTRR"
     gene            complement(37566..38828)
                     /locus_tag="PLP50_05115"
     CDS             complement(37566..38828)
                     /locus_tag="PLP50_05115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPA50965.1"
                     /translation="MRRRVAVTGLGCVTPLGTGAGAFLDGLRSGRSGVRRISLFDPSD
                     LPVRIAAEASGFDPANHVGAKDARHVSRVVPMAIAAAAEALASAGIEVEALPLDERRR
                     IAVLLGSGGGPVEFFERMYGNWYRGELKKASVYAIPTGTIGTLSSEVSMRFGLKGPSH
                     VVSTGCTSSADAIGYAAKAIRYGERDVVLAGGADAVITPGIMTGFGLMQILASSRQEA
                     PETASRPFDAGRDGFVLGEGSWFFVLEEMERARARGARLLGEVAGWGSTCDAYHRVRL
                     DESGVEPARAMTEALGDAGLDVRDVGYVQYHGTGTELNDRIETRATRLALGAAAERVP
                     GSSVKSQIGHPQGASGAAGLAATLLALNAGFLPPTANLDTPGPECDLDYVPRDSRPAE
                     VGVALVNGIAFGSKNSALVVRTARALPG"
     gene            38949..39752
                     /locus_tag="PLP50_05120"
     CDS             38949..39752
                     /locus_tag="PLP50_05120"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA50966.1"
                     /translation="MSDSRFEFRGDLSKTPLPEVLQTVHHYRVPGVVVVRRGVVEKKI
                     YIWNGDVIFATSNDREESLGNVLLGDGKLTQAQFDESVRRLVEARSTEGGRRHGAVLV
                     DMGLLTAEELFAAVTRQVRGILFSLFEWDEGEVTFAVGRFRTDEIIQLEIPTRQAILA
                     GVRALRDARKTVSHLGPSWTILDPSWKPADLGDLALEPGELRLLQQVDGVKTLRELVG
                     LGPSDAAHNAKLLYAFYVLKLIGRRDVTSRTSVKKIHWSTTGGGYAPPS"
     gene            39772..40113
                     /locus_tag="PLP50_05125"
     CDS             39772..40113
                     /locus_tag="PLP50_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPA50967.1"
                     /translation="MPLYEFRCESCGHTMETILAFSDPLPTTCETCGGALKKLLSAPA
                     VQFKGSGFYLTDYGRSGAAGAKEKESGGDGGAKPSGEPKPSAETKAGGDAKPTAAASG
                     SGTGGDAKPAG"
     gene            40094..41941
                     /locus_tag="PLP50_05130"
     CDS             40094..41941
                     /locus_tag="PLP50_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50968.1"
                     /translation="MRSRRADLAAALVAVALAPAFAAADGRPVTAPGGTWLRYPLPGA
                     EVKSLVAEPRAPGLFYAGTALGGVYRSTDGGRTWSAPPGGAPFPGYSVTSLLPDPQRV
                     GTVWAGLTGVVKGGLLVRSDDAARTWSVVRRWAERAEARVVAVAVLRGRKVVAVGGDT
                     GLEISEDGGATWRPSQPPLDPGSGVAFLAFHPLKVGVLYSGSYRHPFRSTDLGRTWKR
                     IAGGMVEDTQVFHLDFSPTDPDDIWAATCGWVYRTTDGAASWTRYKDGLADRRTHVVV
                     RDPRDPARVLAGTTGGLFESRDAGRSFRRISRDSTVVNALVFDPANPAALLVATEAEG
                     ILRSEDGGLTLLESNAGLSEARVSAVAVTASGRVAVARAADGGSGGLWDLDPATGKAE
                     RIAAAPAATIRALLASGERLLAGTPDGVWLAAAPDAPFARVLGVAVHGFAAGPDRLFA
                     ATDGGVFESRDGGASWGRVGDLRSRVDTVMWSRSPGTGAETLAVRSAGRTLWWNGRDF
                     GLDARSGANPRPLSGGFGRPRASAYRPAEPIGLDVDSEKGRLLYRVPGPGADVALALP
                     ERGLHVAGWAGDPRSARGLYLATIGRGLFRFVPSAAGGSASGELSAPAR"
     gene            complement(41922..42425)
                     /locus_tag="PLP50_05135"
     CDS             complement(41922..42425)
                     /locus_tag="PLP50_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPA50969.1"
                     /translation="MTNRARSSTRRDPGRAVLLGVLLLAACSGGRGAPPAAPTPAAGP
                     RVALPSGAVFSLELARTPEEIAQGLMFRESLAPSSGMVFLFDAPEPRSFWMKNCHFPL
                     DMVFALADGTVVDVLENVPPCEADPCPSYPARAPADTVVELAGGEAGRHGVVPGARLS
                     FLSVPGR"
     gene            complement(42391..43848)
                     /locus_tag="PLP50_05140"
     CDS             complement(42391..43848)
                     /locus_tag="PLP50_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA50970.1"
                     /translation="MTGAPPRPAGPRILVVDDEPGITGMLSIVFEREGYDVRTAGSCA
                     EGLRLVGELHPDLVLTDLRMPDGTGFDVLRKTRETDAETPVVMITAYTSTKTAIEALK
                     AGAYDYVSKPFDVDELKHVVGKALERKRLADENQELRSRVEDRPALGPLVGVSRRMRA
                     VFDLVDRVGRTTSTVLITGDSGTGKGLIARAIHRSSARAGRPFVSINCGALPENLLES
                     ELFGHEKGAFTGAVREKKGLFKEAEGGTLFLDEIGETSLSMQVKLLQVLQERVVRRVG
                     GNVEEPIDVRIICATNKDLAEKVADRSFREDFYYRIAVIPLQIPPLRERREDIPLLVR
                     HFLKKVATEQKIPEKKISSEAIRLLEAYSWPGNVRELENLIERTVALEPGPVITTTSL
                     PESFLRPAGVPAEALQTPFDLPMTAFDLEAYLEWIGKRLMQQALERANGVRKEAAKLV
                     GMSERSFRYYAKKYQIGHDDEGEPYDEPGPVVDET"
     gene            complement(43845..>44950)
                     /locus_tag="PLP50_05145"
     CDS             complement(43845..>44950)
                     /locus_tag="PLP50_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA50971.1"
                     /translation="LLAERRREEVARLQALHSSVLSSMSSGVVTADLAGRITFANPPA
                     GELLHRNPAELVGRPLFETGLVWPEILDPTHPPGNEVFRFEGNRTPLGAGAYFGVTIT
                     PLRDGDGRVTGRILIFQNLTEMKKLEGEVRLKEKLAAVGELAASIAHEIRNPLASISG
                     SVQALSATAPPGSSERRLMEIAVQESHRLSAILEDFLKYVKPRDQAIETVDAAGALRD
                     VVTLLTHSDELSERHAVTLKLEPESFPLQADPGQLRQVFWNLLRNAVAAMPDGGTLAV
                     TARAGGGVWSATFSDDGRGMSREDQERLFTPFAHATPGGTGLGLAIVYRIVEEHGGSI
                     RVKSAPGDGTAITLTLPVGGPGARTTEAMGVRA"
BASE COUNT         6082 a        15998 c        16650 g         6220 t
ORIGIN      
        1 agccgctatt catgcggggc atccccgtcg aaccgagctg tcttggacag cagtgcgcga
       61 gtccacgagc gcccaccgcg ctaccaggga gctctttctc tatggaaagg aggaccggac
      121 cgaggcaagc tcacaccctc acgccggcgt gaccccgggg ggaatgatcg tgaaaaaccg
      181 gggtcccaag gtgctggaaa tcgacagcca acgcgaaacg ccggccttca ggccggcgct
      241 cctgttgacc gccttccctc agcgggtcag cggggaaggt cacgtggctc cgcgatcggc
      301 ctatcaggcc aaccgagttc tcagaacggg gctgagggtt caactggttc tgggcgcccg
      361 aaggttagcg gcgagacgac tccgcgggcc gcgccggaca cgcgcccgag aatggggggc
      421 catcgcaccg cacatcgtat tggcctgcgg gccgggccgc gtcgggggcc tgcggccgct
      481 gcgcggctcg cttcgcgagc cctggatccg gcccggtccc cgggccgtcc tctgctatcg
      541 gggagatggc tcgccatctc cccgatagga ggacgacgat cggaaatagc accgcgaaga
      601 ccagcggggc gcgcccgccc gccccggaac acacccgttc gcttacagag aaatcctcgg
      661 cggcacgctc ctatccgctc ggcgttgcgg aggcgctcgg agcagctgcc tggtcaattc
      721 gaatgcgaag atcctcaatg aacttcctgt cggcccagtt cgcggccccg aagatcctgc
      781 taactacggc gcccgtatga tcaacaatca gcgtcactgg tacggctgtc cccaggccgc
      841 tgatatccga gcctggagcg aatcggtata caggaaaacg aagcgtgcgc gtgtgaagaa
      901 acccgcggag cgtagttgcg ctatctcggg agatcgaaac gaacgcgaaa tcgcgatgac
      961 cttcgaagct cgcatagaga gtctcgagcg ctggcaactc ggcgatgcac ggttggcagt
     1021 aggtcgccca gacgttgatg aagacgactt tccctctgaa ggtcgagaga gggacggaat
     1081 gcccctgggc atcctcgagg acagcattga actccagaag cgatgtcgtc gcggcgttcg
     1141 cgttcatccg agggacagga agtgcatctc cgcgggccac cccgccgcct ccggctgcca
     1201 acgtcggcgc gccttggatt ccgttcatca gcttcttgaa cagtgcaccc gccgagtaga
     1261 agccggccgt aaacccaagg gcgaaaccga agacgacgcc tccgagcgcg aagagaagac
     1321 ggatccgcct ctcgaaaccc atccgcggcc ggccgtcgct ctgatgcgcc tcagcgccag
     1381 taagacttag ccgcactgaa ctcccacgac gaggcaggcg cagaagaagc cttggtttcc
     1441 aggaacgggt agacactggc agtcgcaaga gagggtctgg caacagcccc agcacttaga
     1501 attgttgcac ttgttgtttg cgtcctgcct agcgttgtta caggaaatcg acctacggaa
     1561 gttgtagccc caacccgaca ccgaagggcc ggtgcagctc cccccgagtg ttccacagtt
     1621 cgggtttgtc acgatgtcgc acgggggctc ttgctcgaag tcgtcgggga cgcgaccgtt
     1681 ctcagttacc agctcgcccg cctcaccgac gagcctcaac gcttcgcgga agtgctcacg
     1741 tccgaggacg gagacatcgt catcagtccc ctcgacaagc agccactggc gggcggtgga
     1801 gtcgaacatc caggagatcg cacggccgtc gggaagctga atctcgacga agcccagcct
     1861 ttgctgaaag agtacacgca gcccgctggc atcggatcgg tcagcatagt gtgcctcaat
     1921 gaagccaggc ttctccagat caaactgaac cgatgcgatc tctctagctg cggaatcgcg
     1981 gacgtgaacg aggggcgcgg ttgagccctg ttccattgtc acctgaaggt tcgatgaact
     2041 cgctggcttc gtctgatcca ctgaaagtcg atcggcggcg accgccatcg gcgcagagca
     2101 gaggatcgcg agcccgaaga gtgcatggtg gatcgaggtg ttcgtgcgaa gaaatggtga
     2161 taacacagtc ttctcctcga aacgtgaatt gcgagcatga agctcggctt ggtgacgaga
     2221 gggtactcga tagaacgaaa ggcaacaaga agaatggcgg catcgagatt ccttctcgct
     2281 gactgattgt ggatgttcgt atgccgcaag taccgaatgg gtaggtcgga aaggcctgcg
     2341 gccggcgcca atgagtgggt gggagacggg gggaagcgtg cggcaggtcg gtagcgctcc
     2401 gatggctcga gcgtcaatac aggccaaaca atgacctcgt tcgctgggcg tggcgagaag
     2461 cggatctccc ggcgatctga acgagcagta gggtaagaca gcgcaactga tgcctggggt
     2521 cgagcccaag gcgcaacatg ctcgtgcgtt cgtggttcct tgcccggagt acgctccgag
     2581 cctgccgacc gacgcggaga gcaaggcgcc tcgggtcctc gccgctagcg cgccaacggc
     2641 gagtcccgct gcgcgcgcgg actcggccgt tacgttccca ccggaggtgc cacgcgcgag
     2701 tggccctgta cgtccgccga tcgacgaccg acctccatcc cgacagcctc gaagcccagg
     2761 aagagcgctt ccgatcacac gccacggaat cggggcacga agtcgtccgc gtctacagcg
     2821 acagcgcctc cggcaaacgc gtcgagaagc tggacgcttt ccagcgtctc atcggggacg
     2881 tcaagcgaaa gccggacttc ggggccatcc tcgtccgcga cgtcagccgc tggagccgag
     2941 ccgagaacac cgacgaggct ggctactacg agttcatatg ccgctcccac ggcgtccagg
     3001 tcctctacgc cgacgagacc ttcggaccgg agcagtcccc ctacgccctc ctcctgaggt
     3061 ccgtgaaacg ggccatggcg gccgagttca gcctcgagaa ggcccgggtc gtccgctcct
     3121 cccacgcacg cctcgtgcgg caaggcttct ggccgagcgg atcggtgccg ttcgccatcg
     3181 agcgcgtcct cgtcgacgac gccggaaaca ccctcggcac cctcgaacac ggcgaccaca
     3241 aagccctctc gaaccagagg gtcaagatgg ccccggggga ccccacgcag ggcgcgaaga
     3301 gcccggagac gcgaaacgcc ggcatcgctg ccggcggtcg acgggaagac gcggaaggga
     3361 atggcgtccc attggttctc agggatggag tgctccagaa ggggattcgc cgggctccct
     3421 tgcgtgccgt ctccgtagta ctgcacggag tactggttcc cgaacaagtc gcccgcgagg
     3481 gtcgtgtgat aggcgggcac ggtctccgcg tgatccaagt cggtcggaag ggacgtcccg
     3541 agaatcggat atcgccctcc gcgaaatccc attactcgga aagtgccgtc cgggaagtag
     3601 accgtcggat ggcctccgtg ctccggatcg atcggatcga gtcgcaggaa gctgccgtcg
     3661 tgggtgtagg cgaagcagtg ccccgcacca tgttcggtcg ggccgaagca ctttggggga
     3721 tgcgtcggct cgcaggcggg atcagaaatg ctgcaggagg attcgacgag tcggtcgtag
     3781 aactcatgga ttcccccgtc ggtgtcctcg tacgcccagc gggtcgaacc catgccatag
     3841 accgcgtcgt cgacgagagg cgcctccggg gtgctcggct gccagtagtc gatgctcccg
     3901 cgggggattc tcgtctcgac gggcccgagc cgaccgaagt gaaggcgcca accggggcca
     3961 atcgctcggt cgccagtggg gtgctcctca ttctccttac aaacgcgcca caccttctct
     4021 gcctggctgt tgtagacgag cgcgggggca atggagagca gaggcccgac ggcgaccggc
     4081 tcgccgatcg gaatgaggtc gtgaaggttg ccgttgaaga gattgatgtc gtcggaccca
     4141 gagctcgagt agagggtctg ggcactgatc cctgcggcgc ggttggggtg atcgccctgt
     4201 ccgaggatcg gcgtcgcaag acatgcggcc agcagaggac cgacaactgc gggacggaac
     4261 ttcaagcatg ttcctttcgg ggttgggctg agtagatact cagttggctc ttgggcggcc
     4321 gtgatgacgt cttcacgcag ggcacttcag atggcgcccg cgggcgaggc gggcgaattg
     4381 ggaccgcgtc ggaccggaaa ggggcgagac gagggacgtg cccaatcacg cttcaccgcc
     4441 cgggctcgcc gagagggtgg ccctcaacgc ctcctccgtc acgaactcaa gaagcccgat
     4501 ccggtcccga tagagctcgc cgcggaggat cacctcccgg cccttctcgg cgagcctgcc
     4561 gattcccggg agggagccga cgacgagaac gtcggtttcg ggggtcaggt caacatcgat
     4621 ccgcccgccc cgtgctcgaa cctcctcctg tagagcgtga agctcctctc ccgatagccc
     4681 gcacgcgaac gcgaacgagc gaccggcgat taggatggac ttcggggctc ctcgcgcccg
     4741 aggcgggtcg tccctgaaat gcacacacaa ctcccttcca ggaggacggt ctggagagcg
     4801 agccccaggc atcgccgtcc ccctacctct gatgagtgat ctggaggggt gaaagtgccc
     4861 ctggctcgaa gaaatatttc tgctccagcg ccgggaccct gcggcattcg tcgcgcctgc
     4921 gtctcaacaa tccgccgcgt cgggcctccg gaagtggctc ggaacgagtg gcagcgcccc
     4981 acctttccct ccggaggtgc cgcatgaaag tggccctgta cgtccgccgc tcgacgatcg
     5041 acctccagcc cgacagcctc gaagcgcagg aagagcgcct ccgatcacac gccgccgcct
     5101 cggggcacga agtcgtccgg gtctacagcg acagcgcctc tggcaagcgc gtcgagaagc
     5161 gcgacgcttt ccagcgtctc atcggggacg tcaagcgaag accggacttc gatgccatcc
     5221 tcgtccgcga cgtcagccgc tggagccggg ccgagaacac gtcggcaaca ccctcggcac
     5281 cctcgagcac ggcgaccaca aagccctctc gaaccagcgg atcaagatgg cgccggggga
     5341 ccccacgcag ggcgcgaagg gcccggagac gcgaaacgcc ggcatcgctg ccggcgttcg
     5401 tcgatccgca ggcccctcgc gggccgacgg ggaaagtacg atggctccgc aggtaagact
     5461 cgaacttaca acccttcggt taacagccga atgctctacc attgagctac tgcggaacgt
     5521 tctcgaaaga gcggccgcgg agggcggacc ctgcgcggaa ggcggagtct aggagaccgg
     5581 gtggcggagc gtcaagcgag gggccgaggc gggcatttcc ggcccggagc cgtcgatcgg
     5641 tgaccgatcc gcgccccagg ggccgcttcg ccccgtctcc gaccggcccg cttcacctcg
     5701 gcagcctcgt cgcggcgctg ggttcgtggc tcttcgcgcg gcgagcgggg gccgagtgga
     5761 tcgtccggat ggaggacctc gacgggccgc gcgtcgtgcc gggggcggcc gacgagatcc
     5821 ttcgcgcgct cgagcgctac ggactgactt gggacggcga ggtcgcggtc cagtcgcggc
     5881 ggacggtgtt ctacgaggag gcgctcgcgc ggcttcgggc cgcgggccac gcgtacgact
     5941 gcggctgcac gcgagcggag gtggcccggg cggcgggggc gcccgacgcc gcggacccgg
     6001 tcgacgcggc cggggcggtc tacgccggga cttgtcgggc gggtctcccg gcggggcgcg
     6061 cgccgcgcgc ggtacggttc cgcgtcccgg agggaatcgt cgctttcggg gaccgcgtcc
     6121 acggccgaat cgaagaggac gtggcgcgcg cggtggggga tttcgtggtg aaacgggccg
     6181 acgggccgtt cgcctaccag ctcgccgtcg tcgtcgacga cgcggcgcag ggggtgacgg
     6241 aggtcgtccg gggcggcgac ctcctttcgt cgacggcgcg gcagatcgcg ctccagcggg
     6301 cgctcggcct cgcgactccg tcgtacgcgc acctgccgct cgtcctcggc gccgacggga
     6361 agaagctcgg aaagcgcgac ggcgcgctcc cgctcgagac gctcgacgaa cggcgcgtcg
     6421 cggagtcgct cgccctcgcg ctccggctcc tcgggcagga ggtcgcgggg gaggcgccgg
     6481 gcgagatgct cgcggcggcg gcgcgctcgt tcgacgtgac gcgggtcccg acggggccgc
     6541 tccgcgtcgc ggcggacggc gggatcgtca gcggcgcgta ggccggcgtc ggcccggccc
     6601 gggcgacgtc aggacggagg ccgcctcgcc gaccttccgc tcgaacgcgg cgaggtcgtt
     6661 ccgcgcgacg agcgcgcgcc aggtggcgag ctcctccgcg aggaggtcga agagggcggg
     6721 cgtggcgggg tttcgcgcct ggatcaggag cgtcaggtcg ggggactggc ggaggatcgg
     6781 cgcggcgagg aggacgagat tgcggaagct cgtcgaggcg aggccgtcga ggccggtggc
     6841 gctcacgccc ccgcgcgcga ggacggagcc ggcgaggagg gagaggaggt gggcgaggcc
     6901 gagcgtcgtc gcgatccacg ggtcgtgcgc gccgatcggc atcgtcgcga gcttcaggcc
     6961 gtggccggag aagagacgcg ccacgtcgcg cgcggccgcc tcgtgtccgg cgttgcagac
     7021 gatgaggttc tgccccgcga gcgagcggac gccggggccg aacatcgggt gggccgaggc
     7081 gacgcggagg ccgcgccggg ccgccttcgc gtagagcggg aggagcggcc ccttcacgct
     7141 cgccacgtcg aggacgaggc ccgggggcga gagggcgagg agctccgcga ggacggcggg
     7201 cgtcgcctcg agcgagacgg agacgacgac ggcgtcggcc tcggcggccg tcgcgagcgg
     7261 gaccgacggg aagccggcga ggcgccggcc ggggtcgtgg acggcgaggg cgtggccggc
     7321 ggcgcggaag gcgcgcgcga gcaggcgccc catccgcccc gcgccgccga cgacgaggac
     7381 ggtccgaagg ggcgcggcgg cgcggcgacg tccggacgcc gccggcgtcc cgttccgtcg
     7441 ggcggcggcg cgccggggca tcgcgctacc gcgcgacgcg gaggaagcgc ttgccgagcc
     7501 tcacgtagcg cgcggcgccc ggctcgagcg cgaacggctc ggcggggttg tcggccttcg
     7561 tgaaggcgac gccgtccccg gaggtggcga cggctccctc cttcaccttc tgccgcgcga
     7621 cgttcttgct cggggcgagg ccgacggcga cgaggagcga ggcgaggtcg gcaggggccg
     7681 ggtcgggtag ggagacggtc tcggccttca cgggcccttc ggccttcgcg aagcgccgct
     7741 cgaactccgc ctcggccgcg gccgccgcgt cggcaccgtg gaagtcggcg acgagctggc
     7801 gggcgaggtc catcttcacc cgtttcgggt gcttcgcacc cgaggcgacg tcggccttca
     7861 gggcgtcgat gtcgtgcggg aagaggtcgg tcaggagggt ccaccaggtc cacatcatgt
     7921 cgtccgagac ggacatgacc ttcccgaaga tctcgtcggg cgggtcgctc acgccgacgt
     7981 ggttcccgag cgacttcgac atcttctcga cgccgtcgag gccgacgagg agcggcgtcg
     8041 tcatgacgca ctgcggggcg aggccgtagc tccgcatcag ctcgcggccg acgaggaggt
     8101 tgaagagctg gtcggtgccg ccgagctcga cgtcggcctc gaggaagacg gagtcgtagc
     8161 cctggcagag cgggtagagg agctcgtgga cggagatcgg ctggcccgcc ttgaagcgct
     8221 tggagaagtc gtcccgctcg aggagctggg cgacggtgta gcgccccgcg aggcggatga
     8281 ggccgtcggc cccgagcgcg ccgagccagc gggagttgaa gtcgacgacg gtcgtctccg
     8341 ggtcgaggat cttgaagacc tgcgtcttgt aggtctcggc gttcgcgagg acctcgtcct
     8401 tcgtcagctg cgggcgggtc gccttcttcc cggtcgggtc gccgatgagg ccggtgaagt
     8461 cgccgatgag gaagacgacc tcgtgcccga gctcctggaa gtggcgcatc ttccggaaga
     8521 cgaccgtgtg gccgaggtgg aggtcgggcg cggaggggtc gaagccgacc ttgacccgga
     8581 gcggcttgcc gctcttgcgg gaggcttcca gcttcgcggc gagctcgtcc ttctggacgc
     8641 acgtctcgac gcccttgagg aggagctcga gctgatcggg gacgggagcg aagttcggca
     8701 cgcgcggatt ctagtggccg agaggcccgg ggcgggcgac ggagcgccgg cggcctccgt
     8761 acactcccgg cgtggagacg gaagacctca accgcctcat ccgttccctg aacgcggccg
     8821 aggagggccc ggcgggggag gagccgctcg tggagcacgt gcgggagctc gaggcggcgg
     8881 aggccgtcgg gctgcgaacg ggtgctccga ttgcggatgg ggagctctcc ccgcgcctcg
     8941 cggcgctcct gaccgagacg gttcgccgcg gcgggaccga cctcctcctc gtccccgggg
     9001 cgtggcccgt cctccgcctc cacggctcgc tcgagccggt ctcggccgcg gccggcgagc
     9061 cgctcggccc gtcggaggcg ttcgacctcc tctccccgtc gctctcggcc gagcgccgcc
     9121 ggcggtacgg cgccgagggg gccgtcgacc tttccgtccg tctcggcgcg ctcgggcggt
     9181 tccgcgtgaa cctgcaccgg acccggcgcg gcacggccgc ggcgatccgc gtcctgccga
     9241 agcggatccc gccgctcgcc gagctgggcc tccccgagtc gctctacgac ctgacgaagg
     9301 cggcgcgggg cctcctcctg gtgacgggcg cgaccggttc ggggaagacg acgacgctcg
     9361 ccgcgctcgt cgaccggatc aaccggacgc agaagcggca cgtcgtgacg atcgaggacc
     9421 cggtcgagta cgagcacgcg cacggcgcgt cgatcgtcga gcaggtggag gtgggggccg
     9481 acgcgccgac gttcgccgcg gcgctcgtgg cggcgctccg gcaggacccc gacgtgatcc
     9541 tcgtcggcga gatgcgcgac ctcgagacga cgcgcgcggc gctcaccgcc gccgagacgg
     9601 ggcacctcgt cctcgcgacg ctccacacga acgacgtccc gcagacggtc taccggatca
     9661 ccgacatctt cccggccgag cagcaggcga cggtgcgaca ccagctctcg ctcgccctct
     9721 cggcggtcct ctgccagcag ctcgtcccga gggccgacgg gaaggggcgc gtcgtggcgg
     9781 cggagctgat gctcgcgacg gacggcgtga gggcgcacgt ccggaaaggg acgttccacc
     9841 agctgcacac ggagctgacg ctcggccgcc gactcgggat ggtgacgatg gaggagtcgc
     9901 tcgcgcggct cgtccgctcg ggtgcgatca cggaggccga ggcgcgcctg cgcgccgcgc
     9961 accccgacga cctggctgcc cacctccggt agggcggctt actcgctccc gaggcagctc
    10021 tcgaggacgc gggtcgttcc accggggcgg atgtcgaagg tgaccgtcat cgcgcagccc
    10081 gactcggggt gggcgagccg ggcgcggtag gcgccgggcg ggagcgggag gctcgccggg
    10141 agcgggcggg ggccggcgac gcggcggccc gcctcgtcga cgacctcctc gaggacaccg
    10201 cccgggacga gcgcgacgag gaggcggccg gcgggcgcgc cggcggcgga cgggggaccg
    10261 gtgtccctcc ccgcgatcgc cgggcggagg ccttcggcct cggcggccgg ctcgggagcg
    10321 gagacgacga cgtggagggc gaaggcgagc gcggccacca gggcggcgat gccgaagatg
    10381 aggacgacgc gtccgaggag gagctcgcgg gtcaggagcc gcgcggcggg cgcgagggcc
    10441 ggggcgggag cgacggggac gtcctcgacg ggggcttccg ctggcggggt cgccgcctcc
    10501 gggaacggtg cgtcggcgcc ggcgccgtcc tgacggggct cgggcggtgg gacgtggtcg
    10561 aacggggcga gctcggcctt cttctgctcg agctcgcggt ggacggggcg cacctcgtcg
    10621 agcgcctgcc agagggcggc gacgatctcc tcccacgaag aggggcggtc ctcgcggcgg
    10681 cgggcggtca tcctctccac gagcgcgagg agcttcggcg cgggtccgcc gcgggcgggg
    10741 gggagatcga ggcgcggcca ggagcgcgtc aggtgcgcgt tcacgatctc cggcagcgtc
    10801 gtcgcggcga cggcctgccg gccggcgagg agctcccaga cggtgaggcc gaaggagtag
    10861 acgtcggcgc gaaagtcgag cgtctgcccc ttcgggagac cgaggagctg ctcgggcggg
    10921 gcgtaggaga gcttcccgag gaaggcgccg tgctgcgtca ggcctccggc cgccgccgag
    10981 gcgaagaggt ccttcgcgac gccgaagtcg atgagcttcg gggcgaagcg gccgtcctcc
    11041 atggcgacga ggacgttgtc gggggcgacg tcgcggtgga cgatgccgcc gcgggcgacg
    11101 gcggcgagcg actcggcgat cgggatcagg atcgggatca gctcccaggc cggacggagc
    11161 gcgaactggg aaagcggggc gccgcggacg agctccatcg cgagccagcc gtaaccgtcg
    11221 agcgtgacgc cccagtcgtg gaacttcggc agcgccgggt gggagaggcg cccgaggagc
    11281 tcggcctccc gctcgaacct ctcctgcggc cggaattcag ggtcgaggtc ggcgaggctc
    11341 gtgatcttga gcgcgacctc gaggccgtcg gcgtcgcgca gggcgcggaa gaccccggag
    11401 agaccgcccc ttccgaggcg ctcgaggatg gtgtagccgc cgacgaggtt cgggcggacg
    11461 ttcacgggag catcttctca ggagtcggtc cgggggcgga gggcgcgggg cttcgccccg
    11521 cgccctcacc gcggggccgg aggggcggtc ggcaccggct cgaccgggag ggggacgggt
    11581 gcggtcgtct cctgccggag cctctcgatc tccgcctgct gcagggcccg gtcggcggcg
    11641 cgctcccgct ccatctgctc gagctgccgc tgcagcgcct ccttgtcggc ggcgagcgcg
    11701 tcgagcgcgc gctgcatccg cccctcgagg tccttccggc gggcgtcggc gtcgccgaga
    11761 cgccggcgga ggtcggagac ggccttctcg ctcgcgccgg cggtcttctc gagctcgccg
    11821 agcttgcggg cgacctcggc cgactccgcg gcgagcgcgt cgcgctcctg ccgcatctcc
    11881 tcgacgcggg tgccgagctc cttccggacg ccctggacgc tctcgtcggc ggcgcgcgtg
    11941 acgtgagcga cggtctggcg ggtgcgccag acgttgtagc cgagcgcgaa ggcgagaatg
    12001 acgagcgccg ctcccgtcag caggagcccg atcttcagag agcgggtcgc gctcgtgatc
    12061 gtgtcggcga cgacctcgcg gacgaagacg gtccccttct cgacgaaacc ggaatccccc
    12121 tgctcgcggg ccttcgagag ggcgtcctgg atcatgacca tgaccgtctt cggcccgacc
    12181 ggcttctcgc ccgaggcggt caggacgctc cggcgccgcg agccggtgtc ctcggcccgc
    12241 cggcgggagg cggtgtcgtc ggcgcgtgat ggctcggcga cggggaaggg ctgcggggcg
    12301 gtgaagggaa gctcggcgga gacgcgcctc acggtgaaga cggggcccca ggcgccgaag
    12361 cggacctcgc cgccgtcggg gaccggatgg tcgagcggga actctccctg cgcggagagg
    12421 tcgcggttgc cggccgagcg gatccggaac ccggagccct cggccgagat ctcgatgacc
    12481 cgaccgcgag ctccgccgtc gccgggcgcg gccgggtcga cccggagatc gacgccctcg
    12541 gcgtcgccga cgcggacgac ctccccgggg aacgagaagt cacgatcggt gagagacccg
    12601 gagaggaacc ggacgacgat cggcatggcg gcctcctccg cccgtggcgg cggaatgtgt
    12661 accgggatta tccgcccggg gcccgcgccc gggtcagccc aggatcttct cgaggcgggc
    12721 gaagaggaac tcgagggcga cggcgttctc gcccccttcg gggatgatca ggtcggcata
    12781 acgcttcgac ggctcgacga actcgaggtg catcggccgg acggtcgact cgtactgggt
    12841 gacgacgctg tcgagcgtcc ggccccgctc ggcgatgtcg cgccggatgc gccggatgag
    12901 tctcacgtcg gcgtcggtgt cgacgaagat ccggacgtcg aagagcgtcc ggagcgcggg
    12961 ctcggcgtag atgaggatcc cgtcgacgag gatcaccttt cgcggctcga tccggcgcgt
    13021 ctccggcgcg cgccggtgcg tcttgaagtc gtagacgggg acctcgaccg tcgccccgct
    13081 cttcagggcc ttcaggtggc gcacgaggag gtcgctctcg agcgagtcgg ggtggtcgaa
    13141 gttcttgtgg tcgaggacgt ctttcgggag gtcgacccag tcgcggtagt accagtcgtg
    13201 ggaaaggatc gcgatgcggt cctctcccac ccggttgcga atggcgttcg cgacggtcgt
    13261 cttgccggag ccggtgccgc ccgcgacgcc gatggcgagg ggcgccgcgg tcacgcggcg
    13321 ccctccgccg ggcgggtcgt cacttgaagg cctcgagcat ctccttcgcc gtggcgacct
    13381 ccttgccctt ggggtcgagc cggatgtatt ccgcgaggtg cttcttcatg tcggggatct
    13441 tgttgaggtt cacgcacgac atgccgagga ggaagtgggc gttggcgtac tccggggcgc
    13501 cctcgatcac cttgcggagg atcgggtcgg cccccttgaa gtcgcccttg ttgtagaggt
    13561 cgacggcctg gttgtagagg atctcgggct tgtcggggtt ggcggcgatg aacttcttct
    13621 cgtactccag cgccttggcc ttgtccccct tcgccttgta ggcctccatc agcgcgccgt
    13681 agagcgaccc gttgtccggc tcgatcgaga gggccttctc accccactgg atgacgaggt
    13741 cccagtcctt cttggcggcg gcgacctttg tcgccatgtc ccaggcgccg gccttgtcgg
    13801 gcccgagctc ggtcgccttc ttcgccccct cgatcgcccc gtcgaggtcg cccgcctgga
    13861 acttctcgac cgcgccgttg aaggcgagcg tgtacgggtc gacgacctcc ttcacgagcc
    13921 ccttctcgac ggcctggtcg gcggtcagga gctggacgtc gagcgtccct tcggagccga
    13981 tcccgacctt cttgttctgc ttgatcggga tgtacccctt cgcttcgacc aggtagtcgt
    14041 aagcgatcgt ggagtcgttg aggatcgtcc cccacttccc gtccttgtcg gtcgtcagaa
    14101 cgaccttgaa cttcccgaac gccggggtcg tgatcgtcac ggtcgccccg acgatcggtc
    14161 cccccttcgc gtccgtgacc gtccccacga tccgggcctg ggggcccgcg agggcgatcc
    14221 cgctgacggc gatcaggatc gcgatcagcg cggccgacag cgtcttcttc tggttcatct
    14281 ctacctccgg gggtggaccg tgccaccaac ccgtcattct agcgaaagcc gtgccgcggc
    14341 ggccgccccg agacgacgcc gcccgcactc cggaggggac ggcgcggcgc ggcctttccg
    14401 acggtcgtcg tcggccccta aactccgcgc gtgccgaaag ggctctttcg atggctgcgc
    14461 ggagggggac ctcccgccgg ggacgatccc cggggcgccg atcgcctcgc ggcggcgccg
    14521 gagcgccggc cgggcgtgac ggtccacgcg gcggggtcgc tcgccgagct cggcgccgat
    14581 gcgggccggg gcggagtctc gcgcctcggg ccgttcgccc ctctcgtcac ggacggcggg
    14641 tgcgcgtggg ccggcgtcgc cctcctcggc gagacggcgg gggacgtctt cttcccggcg
    14701 gcgggcgcga ccgggctctc ctccggcgtc gccgcgctcc tcgacggcgt ccggacggaa
    14761 gaggaggacg cgccggcgag gatcgcccgc ggttccctcg ccggctccgc cgtccccggc
    14821 gtcacggggg cgcgcctcct ctttcgcggc ggcgggcgcg ccgtcctccg cctcggtccg
    14881 ccgccggccg cgcgctgggc ggtcgaaggg ctgcggagcg tcgcggtcct ctccgggaag
    14941 ctgaccctcg tcgacggcga ggaggcgcgc gacgtgcgcg cgggcgaggt ggcgttcgtc
    15001 gcggagcccg ccgcgacgct ccacgtccag gcggggaacg acgcggccct cgcggtcggc
    15061 ttcggccggc ccgagctctc ggtccggctc gggtagcctt ctcagccctc gtgccggggc
    15121 gctcggacgc gccggccgag gagcgcggag agcgcgtcgg cgctcgagcg gaaggcgagg
    15181 tcgagccggt cgacctcctc cggcgcgaac caccccacct cgagggtctc gtcggcggca
    15241 tacggctccg ggctctccga gaggagagag gcccggtagg ccgcgacgat caccgggcgt
    15301 cccggatagg agtagacgcc gaggagccct tcgatccgcg cgagcgcccc gcactcctcg
    15361 cgcgtctcgc gcagcgcggc ctcctcgagc gtctcgcccc ggtcgacgtg gccgccgggg
    15421 aagacccaga gtccgtagcc cggctcgatc ccgcgccgca cgaggaggac ccggccgtcg
    15481 cggacgacga ggcagccggc ggcgaccttc gggtcgatcc agtgaaccgc gccgcacgcg
    15541 ggacagacgg ggacgacctt gccgtccccg gcaccgaccg aacgttcctc gagcctctgt
    15601 ccgcactgcg agcaatagag aggacgcgcg tacacgctcg gagcctaccg cgggacccgg
    15661 cgcgggcgcc ggagcggagg gaaccgtgcg ttccgtcacg gaactcgtcc tgcgcgtccc
    15721 gcgccgagga cggcgcgcct tgacctcttc ccggacggtg accagaatac gcggctcgaa
    15781 tggcgtgccc ggaggtcgtg ggatgagcag gcactggacg caggacgaaa tccgctggct
    15841 gaacgccaac gcggaacgca tggacctccc gaccgtctcc cgcacgctcg gcgtgcctct
    15901 cgtcgaggtc gagaggaagc tcgccgagct tcgggcggac gcggcctcga gccagaagaa
    15961 gccgcccgcc acgcaccggg aggccgcccg ggagctgacc cacgcgcgga agctgttcga
    16021 gaaagggatg gagcacctgc accgccggga gatgtccgcg gccgccgcct gcttctccga
    16081 gctcctcgag ctccagcccg acgagcggga gctcgtcgac cgcgcgcgga cgttcctcgc
    16141 cgtcgcacgg aacgggaagt ccacgaagga gaagccgctg cgcgacccgg cggagatcta
    16201 ccacgccgcc gtcttcgaga agaaccgcgg gaactgcgcg acggcgatcg agctcgtccg
    16261 gtcggcggac gctcaggccg acccggacgg gcggctcgcc tacctcgcgg cgtgctgcta
    16321 cgccctccac ggccgccccg acgaggcgct cgagagcctg cgggcggcga tcgccgcctc
    16381 ggaccagaac cggatccagg cccgcctcga ggcggacctc gcgccgctcc ggtcgcagcc
    16441 tcggttcgag gcgctcgtcg ggcggggctg accgtgcccg ccgctaccgc cgtcgtcctc
    16501 ctcgccgccg ggcgcggagt ccggatgaag tccgcccgcc cgaaggtcct ccacgaggcc
    16561 gcgggccggc cgctcgtcga ccacgtcgtc gggacggcgc gcgccttcct cgcctctcgt
    16621 cccggctcga ggctcgtcgt cgtcgcgggc gcggggcggg aagagctgat tccgcacctc
    16681 gcccggaccg cgcccgaggc gctcgtcgcc gtccaggacc ctccgcgcgg caccggcgac
    16741 gccgtccgcg tcgcgctgcc ggcgctcgga gacgcggtgc gcgtcgtcgt cctggccggc
    16801 gacgtcccgc tcctgacggc cgcctcgctc gcccggctcg acgaggcgct cgacgccggc
    16861 gccgggatcg ccgtcctcac cgcgctcctc gcggacgcgg gcgcctacgg ccgggtcgtg
    16921 cgcgacgcgg cggggcggtt cgagaggatc gtcgaggcga aggacgcgag cccggaggtt
    16981 cgggcgctcg gcgagatcaa ctccggcatc tacgcgttcg accgcgccct gctggagagg
    17041 gagctcccgc gcctgacgga cgacaacgcc cagagggagt actacctgac ggacgtcctc
    17101 ggatacgccg tcggcgacgg cgcggcggtc gcggccctcc cggtcggcga cccggccgag
    17161 gtcctcggcg tcaactcccg cctcgagctc gccgaggtcg acgcgctcct tcgccggcgc
    17221 gccgcgcgcg cggcgatggc ggccggggcg acgctgatcc ggcccgagac gatcacgctc
    17281 gacgacgggg tgacgttcgg accggacgtc gtcgtcgagc cgttcgcgac gatcaccggg
    17341 gagacgtcga tcggcgccgg gacgcggatc gggcagggat gcatcgtcgg gtcctcgacg
    17401 atcggcgcga acgtctccgt ccgcccctac agcgtcctcg agaaggccgt cgtcggcgac
    17461 ggcgcggtcg tcgggccgtt cgcgcggctg cgcgaaggga ccgatctcgg cgagggggtc
    17521 cacgtcggga acttcgtcga gacgaagaag gcacgcctcg agaagggcgc gaaggcgaac
    17581 cacctcacct acctcggcga ctgccgcgtc ggggagcgga cgaacgtcgg ggcgggcgtc
    17641 atcacctgca actacgacgg ctacgggaag cacttcaccg acatcggagc cggcgtgttc
    17701 atcggctccg acgtccagct cgtcgccccg gtcgcggtgg gcgacggggc gctcgtcggc
    17761 gcgggctcga cgatcacgaa ggacgtcccc gcgggcgcgc tcgccacctc ccgcgttccg
    17821 cagaagacgg tcgagggggg cggcacgcgg taccgggaga ggaagacggc ggcgaaggac
    17881 cggaaggggt agcgcgatgt gcggcatcgt cggatacgtc gggccgcgcg aggcgacgcc
    17941 gatcctcatc gacggcctga agaggctcga gtaccgcggc tacgactcgg ccggcatcgc
    18001 cgtcctcggg gcggacggcc tcgtccgcgt cacccggagc gaggggaagc tcgggaacct
    18061 gaccgcgcgg atcgaatcgg agccgccggc cgggcggacc ggaatcggcc acacccgctg
    18121 ggcgacgcac ggccgcccct cggaggagaa cgcccacccg caccgcgacg cctcggggcg
    18181 ggtcgtcgtc atccacaacg ggatcatcga gaacttcctt ccgctgaagc gggagctcct
    18241 cgcccgcggc gtggcgttca cgtcggagac cgactcggag gtcatcgccc aggcgatcgg
    18301 cgccgcgcgc gcggacgcgc cggcgaagcc gttcggagag gtcgtgagga cggtcctttc
    18361 gcggatgacg ggcatgtacg ccgtcgtcgt cctctcggcc gacgagccgg aggtcctcta
    18421 cgtcgccaag aacggccccc cgatcgtcct ggggctcggc gcggacgaga acctcgtcgc
    18481 ctccgacgcg acggcgctcc tgccgcacac gcgcgacctc gtcttcctgg aggacggcga
    18541 cttcgtccgc ctcacggcga cgtcggtcga ggtggcgggc ctcgacggcg cgccccggag
    18601 ccggccggcg cggcgcgtcc cgtgggacgc cgtcgccgcg gagaaggggg gctaccccca
    18661 cttcatggcg aaggagatcg ccgagcagcc gacggtcgtc gtcgagacgg tcgggcacaa
    18721 gctctcgctc gagaccggcc gcttcagcga ggacgcgatg ggggtctccc cgcgtctcct
    18781 ctccgaggtc gaccgggtcg tgatcgtcgc ctgcgggacg agctggcacg ccggcctcgt
    18841 cgggaagttc ctcctcgagg aggtggcccg cgtcccggtc gaggtcgact actcctcgga
    18901 gttccgctac cggaggaacc tcgtcgggcc gcggaccctg gtcctcggga tctcgcagtc
    18961 gggggagacg gccgacaccg tcgccgcgct caccgacgcg aagcgggcgg gcgcgccgac
    19021 cgtgggcgtc gtcaacgtcc ccggctcggc gatcgcgcgc ctcgtcgacg gcgtcctcgc
    19081 gacgcacgcg gggccggaga tcggcgtcgc ctcgacgaag gccttcacga ctcagctcgt
    19141 cgccctcgcc ctcctcgcgt actacgcccg ggcggcgcgc gacctcggcc gcctcgagcc
    19201 gggggacccc ttcctcgacg gcctcgccca ccttcccgtc gcgctccagc aggcgctcgc
    19261 cctcgagccg gcgatcgaga agctctcgcg gacgctcgcc cacgcgcgcg acgtcctttt
    19321 cctcgggcgc ggggcgcact acccggtggc gctcgaaggg gcgctcaagc tgaaggagat
    19381 ctcctacatc cacgccgagg ggtacccggc cggggagatg aagcacgggc cgatcgccct
    19441 gatcgacgga gagctccccg tcgtcgccct cgcgccgcgg gacaccctct acgacaaggt
    19501 cgcctcgaac ctccgggagg tgaaggcgcg ggaggggaag ctcgtcgccc tcgtctcggg
    19561 cggcgacgtg gacgtcgcga ccttcgccga cgaggtcctc gaggtgccgc tcgtccatcc
    19621 ggcgctccag ccgatcgtct ccgtcgtccc gctccagctc ctcgcctacc acgtggcgcg
    19681 gcggcgtggc tgcgacgtcg accagccgcg gaacctcgcg aagtcggtga cggtggaata
    19741 gcggcgggag gaagggcgcc ttcctcgcgc tccggcggtc cgcggcgcct gcgcggtatc
    19801 ctccgcgtcc cttgaacgat cgcgagaaag ccctcctcga aggtctcaac ccggagcagg
    19861 cggccgccgt ctcggccggg ccggggcccc tcctcgtcct ggccggcgcc ggctcgggca
    19921 agacgcgcgt cctgacccgt cggatggcct ggctcatggc gcacggcacg ccggaatccg
    19981 ccatcgtcgc gctcacgttc acgaacaagg ccgcgggaga gatgaaggag cgcgtggctc
    20041 acctcctcgg ctcgaggccg ccccggtcgt tcgtcgggac gttccacgcc tacggcgtgc
    20101 ggctccttcg gcgcttcgcc gtcgaggcgg ggctgacgcc gggcttcgtc gtcttcgacg
    20161 ccgacgacca gctggccgtc ttccggcagg cgctcaaggc cgccgggatc tccgacaagg
    20221 cgctcacgcc gaaggcggtc gcctcgaaga tctcgcgggc ccgcaacgcc ggctggagcc
    20281 gtgacgagta cccgcgccgg ttcggcgact tcgtcgggac ccgcctcgcc gacctccaca
    20341 cggcctacga gaaggagctg aagaaggcga acgcggtcga cttcgacgac ctcctcgtga
    20401 ggacccgggg cctcctcgag aggagcccgg aggtcctcgc cctcctgcgg cggagcgtca
    20461 cgcagctcct cgtcgacgag taccaggaca cgaacggcgc ccaggccggg atcgtgaggc
    20521 tcctcgccgg ggaggcgcgg aacgtcttcg ccgtcggcga cgaggaccag tcgatctacc
    20581 gctggcgcgg cgccgacgtc tcgaacatcc tcgacttcga gcaggacttc cccggcgcga
    20641 ggacgatccg cctcgagcgg aactaccgct cgacggcgcc gatcctggat gccgcggggg
    20701 ccgtcgtcgc cgagaaccga cgccggctcg ggaagaacct gaaggcgacg aagccgggcg
    20761 gggagccggt gacgctcctc gtccttcccg aggagcggga cgaggcgcgc gaggtcgtcg
    20821 agcggatctc ccgcgtccgg tcggtccgac ccacggccga ggtggccgtc ctcttccgga
    20881 cgaacgcgca gtcgcgcccg ttcgaggacg agctggtgag gaaccggatg ccgtacctcc
    20941 tcgtcggcgg gacccgcttc tacgagcggg ccgaggtgaa ggacgccctc gcctacctcc
    21001 gcctcctccg aaaccccgac gacgacgtct cgttccggcg cgtcgtcaac gtcccggcgc
    21061 gcggcatcgg cgcggcgacg ctggaggggc tctcggtcgc cgcctcggcg cggacgagct
    21121 cgctcttcgc cgtcctctcc gcccttcccg acggcctgac ggagcgggcg aagaaggcgc
    21181 tcgtggagtt ccgggacctc gtctcctccc tccgcgagga gttcgcggcc gaggaggcgg
    21241 gctcggcggc ggcggtcgcg gccgtcctga cgaacaccgg cctcctcgcg ctctatgaag
    21301 ggtcggacga cccgcaggac caggcccggc gcgagaacct cgacgagctc ctcgccgccg
    21361 cgcgcgagca cgagcggtcg ggcgccgagg gcgacgaggc ggaccgctcc ctcgccgcgt
    21421 tcctggacgc cgtgacgctc cgctccgacg cggacgacgt cgacgagcgg aaggggatcg
    21481 tcctgatgac gctccacgcg gcgaaggggc tcgagttcga cgaggtcttc ctcgccggga
    21541 tggaggacgg gtcgctcccg cacttctcct cccggcagga cgaggacgag ctcgaggagg
    21601 agcggcgcct cgcctacgtc gggatgacgc gggcgaagga gaagctgacc ctctcccgcg
    21661 tcctccgacg gatggtccac ggcgagtgga cgagccgcga ggcttcgccg ttcctcgacg
    21721 cgatccccga gcagactctc gccgtcgtcg accggacgtt cggcccggcg tcggccgacg
    21781 ggttccgcgg cgcggggcgg ggcggcgacc tcttccccga ctacgaaggg gagtcgcagg
    21841 agcggtggac gccgcaacgt ccggcggcgc ggccggtcgc gcgccctgcc gcgaggccgg
    21901 cgcgggcggc gatcccgacg atgcgacgga cgccgccgcc gcccaccgcc tcgggcttcc
    21961 gccgcggcgg gcgtgtccgc caccccgagt acggcatcgg cgtcgtcctg ctcgtcgagg
    22021 ggtcgggcga cggggagaag ctgacggtct acttcgaccg cgcgggccgg aagaagttcg
    22081 tcgcccgcta ctcgaacctc ctcccggcgt agccgcggga gccgcggccg ccgcccggtc
    22141 gggcgtcagc gcggcgccgc cggctcctcg gcggcggccg cctcgaggag ggcacgctcg
    22201 gcgtcgaccc aggcgcgctc ggcctcgagg agcgcccgcc agcgacgacg cgccgcggtc
    22261 gagccgggcg acgccctctt cagggcggcg acgacgagcg cgcggtactc caggaggcga
    22321 gcggcgcgcg cctcgcgagg ccgacgctcg aggaacgcga tccgcgcgag cgcggcgtcg
    22381 cgggggcggg gcagcgcgag cggttccgcg agccactcgt ggagctcgcg gcgaccggcg
    22441 ggcgtgatgc ggtacacgcg gcgcggcggt cccttctccg agcggcgcct cttcatcacg
    22501 acgaagccgc cgcgcagcag gcggcgcagc gcggggtaga tctgcgggag ctcggcgtcc
    22561 catccgagcg agaggtcgcg cgacatgcgg ctcttcagat cccagccgct cgccggagcg
    22621 cgcgagagga gcccgaggag gacgtgcggg agcgacacga cgaaagtata aaagtctttc
    22681 tataccgggg tgcaaaacgc ggaggattcc ctgtggaatt cggggtctgc cgagagcgct
    22741 gtcgcagcac ccgtcgaggc aagaagaaag ggggcccgtt cggcccccgt atcccggaaa
    22801 tccttgaaat gtaaagagat gagcctgaaa ggcctacttc ttggccttct tggccggctt
    22861 cttcgcggcc ttcttcgcgg ccttcttggc agccttcttc gtcgccatgg ttactgtcac
    22921 ctcctcttgg cagattcgat tgacagtccc ggaagccgcg tgctacgttg accttccctc
    22981 gaaggacggg ggcccgcgac cttcggccct gtcgactgcc cgtcagtcat gggtttcagg
    23041 gttccaggtt ttcccgttca ccggtgaagt gattgccgtc gcttcgccgg ttcttttttt
    23101 tccctcggga attgtagaag aacgagaacc ggatgcaagg atttttttct tgacgcgggc
    23161 ggattttctt ttccccgcaa gcccgagtgc gaggagcagt cgatcggacg ccgcgtccgt
    23221 cccccgctcg cgcgcgagcg gacggaacgg gtagcatcgc gcgcgatgtc gaacgtcgaa
    23281 gcctcccctc tcgtcctcgc ccactcgccc gactccgacg acgcattcat gttcttcggt
    23341 ctgcgcgcgg aagcggtcga cacggaaggt ctcgccttcc gtcacgagct cgccgacatc
    23401 gagacgctga acggccgcgc ccttcggggc gagctcgcga tcaccgccgt gtcgttccac
    23461 gcgtacgcgt acctggcccg gacgtacgtc ctcctgccgc acggcgcgtc gttcggagac
    23521 ggctacggcc cgtgcgtgat cgcgcgcgag gccgacgacg ccccggcgac gctcgagtcg
    23581 ctcgccggca agcgggtcgc gatccccggc gcgctcacga cggcggcgct cgcgctccgg
    23641 atgcgcgtcc cgctcgccgt gccggtcctc ctgccgttcg accgcgtcgg cgacgccgtc
    23701 gccgcgcgcg aggtcgaggc cggcgtcgtg atccacgagg ggcagctcac gtggaaggag
    23761 gaggggttcc gcctcctcgg cgacctcggc gccctctgga cgaaggagac ggggcttccc
    23821 ctcccgctcg gagggaacgc cgtccggcgc gacctcggcg acgagacgat cgggaagatc
    23881 tcccgcgcgc tcgggcgctc catcgagtac tccctcgcga accgggaggc ggctctgacc
    23941 gacgcgatgc gcttcgcccg cggcctcccc cgagagaagg ccgaccgttt cgtcgggatg
    24001 tacgtgaacg actggacccg ctcctacggc gaacggggtc gcgaggcggt gcggctgctc
    24061 ctcgacaggg ccttcgaggc gggtgtcgtc ccgtcgcgcg tcgtgcccga gtgggtcgcg
    24121 gaaggatgag cctcgagcgg ttcacgcgcg gctccgcgct ccgggccgcg ctcctcctcg
    24181 tcgtcggcgc gctcctcggc gcgctcgtct tcttcgccgg cgcggccggg accgtcctcc
    24241 gggaatcccc gtcgcgccac gcgggcggag cggtgaaccg cgccctcctc gacctgctcg
    24301 acctctcgac ctacggcgtg gcggcggtcg cgttcgtcct ggtgctcctc ctcgaccgcg
    24361 tcgagccgct cgggaaggtg cagaaggggc tcgcgctccg cctcctcgcg gtcctggccg
    24421 cggccgcctt cgcctcgcac gaggtgatca cgccgcagat gatggcgctc cgcgaccgcc
    24481 tcccgacgct gatcgacctc ctgccggcgg gcgacccggg gcgaagggcg tggggccgcc
    24541 tccacgcgtt ctcctccggg atcctcgtcg tccggatcgc gaccgcggcg ttcctcttcg
    24601 tcctgctcgg caggcggcgc cgccggacga tcgcgacgac ggaggctccg gcgggcgtcc
    24661 cgcccgcggc ctgagcccgc ggcgggagcg ccccgctcag gccggcttct tcgccgtccg
    24721 gctgcggagg gcgtcgaggc gccgtcgcgc gtcgcggttc tcgggctcga gctcgaccgc
    24781 gcggccgaac gccgcgacgg cccccttcac gtcgccggag gcggcgagcg cctcgccgag
    24841 ggcggcctgc gcgcgggcgt cacccccctg cgaggcttcg acggcgcttc gggctcgcgc
    24901 gaccgcgccc gaatggtcct tcaggcggac gaggagggcg gaggcgcgca cgaggagagc
    24961 cgggtcgttc gagacgctcg cgagcgcgac gtcgagcgtc ttcttcgcct cctcgccctg
    25021 ccgggcgaag gcgagcgtct cggcgagcgc caggaggcgc gaggcgcgac cccaggcgcc
    25081 gcccgcggcc ttctcgccga gtgcgacggc gcgccgcgcc gcggcgagag cggcctccgg
    25141 cttcccggcc gcgcgctccg ccgcggcgag cgccacgtgg aggtcgacgg cgagcggcgc
    25201 gtcctcggga atcagcttca gccccgcgag ggcggaggcg cgcgcggccg cgccgttccc
    25261 ggcccgcgcc tgcgcctcgg cggcggcgag gtacaggagc gccgcgcgcg ggtcggcggc
    25321 gaggcgcggc gcgagcgact cgatcaggtc gagcgtctcg gccgagagcg cgccggcccc
    25381 gtgctcgagg ctcacgagat agaggaacgc ctcgcgggcc ccctcctcgg cgagcgacgg
    25441 gtcctcctcg acggcgcgga gcgcgatcgg gcgaagccgt ccgaggtcgt tgcgctgggc
    25501 cagctgcgcc gcgaggcctc cggcgagcgc cgggtcccac ttctccttca gctgcgcgac
    25561 cgcggtggcg agcggagcct tcccggcgac gatgaggtcg gcggcccgaa ggaactcctc
    25621 cgggccgaag actcccgaga gccggtcgat ctcgttgccc gcggggtcga tgaagaggac
    25681 cgtcgggaac gcgcggacgg cgtagcgctg cgagatgcgg cgcccctctc ccttctccgc
    25741 gtcgagcttc acggcaacga cgttcttccg ggcccactcg ccgaccttcg ggtcggcgaa
    25801 cgtcagccga tcgagctgct tgcacgggcc gcaccagacg gcgtaggcgt cgaggaggat
    25861 cggcttcttc tcggcgcgcg cgcgccggag cgcctccgcg aacggccttc cctcggagaa
    25921 cgcgatcccc tgcgcccgcg acggagcggc cggagacgcg aggacggcga gggcgaaggc
    25981 cgcgaggagg atggaacggc gcatgggccg ggatgtaagc acgctccgtt cccgagggtc
    26041 aaacggagaa gcggcgcggt cccgcgagcg tggcccctgc cctcctcctc tcgcggcgcc
    26101 gacgcgactc agaacgcggg acgaagcggg tggggaggct cgccggccgc ctcgccggga
    26161 tacccgaccg actcgaggaa gaggccggac ggaggcacgg tgagccgggc gacctcgacg
    26221 ctgcgctcca cgaggaggcg cgccacctgc gcctcggtca tccgcccggc gccgacctcg
    26281 agcaggacgc cgacgacgcg ccggaccatc ttccagagga agtgcgacgc ggcgatccgg
    26341 acgaggacga gcccgtcggc ctcggcgggc gcggcgacgc gcgcctcctc gacgacgacg
    26401 atcgtcgagt cgtgcccttc ggggttctcg cagaagccgg cgaagtcgtg ccgcccggcc
    26461 aggagccgcg cggcgcggtc catcgcggcg acgtcgagag cgtccttcga ccagtagacg
    26521 aacggcttgc cgaacgcggt gcgccgcgtc gagatccggt agcggtagac gcgccggacc
    26581 gcgtcgtggc gggcgtggaa ggagggcggc gcgggagaga gcgcgaggac gttcacgtcg
    26641 aacgggagga gctcgttcca gcggcgcagg gaagcggcgg catcgacgcg cgcgcggggc
    26701 gggaggtgga cggaggcgac ctggccgagg gcgtggacgc ccgcgtctgt ccgtcccgct
    26761 ccgtgcacgc ggacgccggg cccccagagc tcgcgggctg cggcgaggag gacaccctgg
    26821 acggtgcggg cgttcacctg ggcctgccag ccgcggtagc ggctgccgtc gtactcgatc
    26881 gtcgcgcgga ggacggcgcc cgccggcgcg gaggtcttcg agggggcgtg gctcgggatc
    26941 acgccgccat tgtcgcaggg cggaggcgcc gggctctatg atccccgaga gctccgatga
    27001 gaaggcgctg gacgatccac ctctccgtcg aggcggactt cccttcggtc cgcctcctgg
    27061 cggccgcctg ccacggcgtc ctcgccgagt gggggcttcc gaccgagcgg cggtcgctcc
    27121 tcgagctggc ggtcgtcgag gcggcgacga acgtcgtccg ccacgcctat cgtggccgcg
    27181 gcggccgggt cgaggtcgag ctgacgcgag agggacgcac catctcgctc tccgtcgtcg
    27241 acaccgggat cccgttcgac ccgacgcgcg tcccgccgcc gcgcgacctc gaccccgacg
    27301 acccgtcgac gtggccggag gggggcatgg ggctcccgat catccgcgcg gcctgcgacg
    27361 agctccgcta cgtctcggac ggcgcgcgga acgagctgac gttgcggctg gccgtcgacg
    27421 agcccgagga gacgtgagcg ttccggaccg tcccttcacc gccgccgccg tcccggccgt
    27481 cgagcggcgt tgccgggaga ctctcgccgc gctcgtctcg cggctcgggg aggtgacggg
    27541 cgacggtctc gccggatcgg cgctcggcgg ggcgctcgga cggggcgagg cgccgacgac
    27601 ggtggacgcg gagggggcgt tcgtcacgcc cgtcccgttc gagctcctcg tcgtcctgga
    27661 ggcgacgccg ggacgcgcgg cgtcgctcgc cccgcgcctc cggcgcgagc tggcgacgac
    27721 ggcgcgcgcg cgatcggccg acgtccgtct cgccacggcg gcccgctcgg cgctcgcgca
    27781 gctcccgccg acgatctcga acctcgagac ggccgccgcc gggcgcgtcg tcgccggccc
    27841 cgcggcgttg ctctcgcccc tcgccccgct cgccggagcg ccgcccgcgg cggccgaggc
    27901 gctcgcgctc ctcgttcgcc gcggcgcggc cctccacctg gccgagcgag gcctctccgg
    27961 gcgcgccccg tcgcgcgccg ccctcctcgc ggggcaggcg gcggtccgcg ccgtggacct
    28021 cgccctcggc gccgcgctcc tcgtctcggc gggccgtttc cgtccgaccg acgccgcccg
    28081 cgacgcggag ctccgcctcc tcgccgcgcc ggcaggggac ggtcccgccc cttccggctt
    28141 ccatctccgg atgtcgcgga cccgcttccg ggacgtcgtc gaccggcacc gtgcggccgt
    28201 cgaggccgcg cgcgaggtgg acctgccgga caccctcggt gaggcgcggg cgcaggcggg
    28261 ccgggcgacc gaccggttcg tggaggtcct ccgtctcctc gaggaagagc ggctctcgcg
    28321 tccgctgccg agctggaccg actacgtccg cgcgctcgcc gagaggcacg gcggctcgcc
    28381 gggacccggg ctcttcgaga cccggcgcga ggaggagctt ccgtcgcgcc ggagcgtccg
    28441 cgcctggccc gtcgccgagc gcctcgcgcc ggccctggcg acgctcctcg actgggaccc
    28501 gggcgacctc gacgtcgtgc cgctcctcct cgacctcccg gccgacgcga cgaaggaggc
    28561 gctcggcgcg cgcctcgcgg cctgggccgc gtccgcctga ccgagggtcc gccgcgggcg
    28621 gggccgtata ctccgccgcc gtgacgtccg tgaccgagat ggcggacgac ccgggggcgt
    28681 ccggacgtct cctgttcgtc ctggccctcc tcgcgggcct cctcctccgg ctcgtgcagc
    28741 tcggctcgcc cgatctcctc ggacccgaag aggggccctg ggccgtcggc gcccgcaaca
    28801 tcgtcgaggg agggctggcc cagctcctcg ccctgtcgtc cacgccgctc ggcgagccga
    28861 ccgggatccc gatcttcttc ccggccctcc tcgcggcggc cgtgaagacg gtcggcgcct
    28921 acgaatgggc gatccgtctc ccctcggtcc tcttcggtct cgtcgccgcc ttcgtcctcg
    28981 agcggatcgt caggcgtggc tacggccagc ccgccgggca catcgccggc gcgttcgcgg
    29041 cgctcgcctc gcccctcgtc ctctcgagcc gcaccgcgac ggtcgagccg acgctgacgc
    29101 tgctcggcct cggcgggatc atcttcgggc tccgggcgtt cgaggaggac agcccgggcg
    29161 aggcgttgcc gtccggcgtc ctcttcggac tcggcttcct ggcgaaaggc tatgccgtcg
    29221 gcctcttcgt gatcccgctc ctcctcgcgc tcctgttccg cccgcgcctc ttcacgctcg
    29281 gccggacgaa gcggacgctc gcgctcctgg ccggctcgtt cttcctcacg gccggctcgc
    29341 acctcctcct cctggcgctc gtccacccgg cctccctcgc gccccacctg tcgcgcgcgt
    29401 tcgggagcgc gcccgacgcg ctccggcagc tgtccgacgt cgccctcgtg agcgccgacc
    29461 tgcggatgat cgtcaagtcg ctcttcgtcc tcctcccgct agtcggggtc ggcatcgcct
    29521 acctggcgcg gccgctcggg gaggaggagg tcgtctccgg cgcgacgggg ggcgagcggc
    29581 ggctctcgca catggcgctc tggtgcgctt acggcgtcga gctcgtcgtc ctcgtcgccg
    29641 tcgccggctc gctcgagctc tcctcgacgc cggtcctccc cgcgctctcg gcgctcgccg
    29701 gcctcggggg cgcggccctc ctgacgcgcc cgcgctcgcc ccggcgggtc cgcttcgaga
    29761 cgatcgcgac cgtcgtctcg ggggcgatcg tcctcgtcct ggcggtgctc ctcgtctgga
    29821 cggcccggga gccgctcttc aacgggggcc gggcggcgcc gttcacggcc gccggcgccc
    29881 tcgcggcgat cgcgctctcc acggccgggg ccgcgtggct cgtctcgggc gacgcggcac
    29941 gccgcttcgg gcgccgcacc ggcctcgcct tcctctccgt cctcctcgtc tccgaagggc
    30001 tcgccgccgc tcgcgcgatc cgacacgacc tcctcgcgca ccggacgggc gtccgggccc
    30061 tcgccgacca gatcgcgccc gagctcctgc cgctctcccc ccgcgccgtc accttccgcg
    30121 cgcccgagcc cgcggcgatc gcgttccggc tcttccggac cgggaccacc tgggccgacc
    30181 gtccgacggt cgcgcagatc gccgcggagg cgcgcggcgg gacgaccgtc gtctgggcgt
    30241 ggcgggaggg gacgaccgac ccggtcgccg cgcccgcgcc gctcgtgagg gcctggctca
    30301 tcgtgaacac gcgcgaggtg acggccgagg tggacgcccg cgccgggcgg acgacggggc
    30361 tgcgcgcctt cgtcgcgcgc cctccgtcat aatcgacggt cctatgacga ccgtgctcct
    30421 cgcggaccgc gacggccgcg ccctcggccc gctcgcggac aagaccgtcc ccgccctcct
    30481 cccgctgcgc ggcgcgccga tcctcgagcg cgcgctcgag gccctcgtct cggccggcgt
    30541 ccgctcggcc ctcgtcgtgg tcggcccgcg cggctccgag atcgagaagc gcttcgggaa
    30601 ggggatccgc tgggggatcg ccctcgagta cgtccgccgc agcgaggacg agacgaccgg
    30661 agcgatcctg cgccgcctcg agcaccgact cgacggcgag actctcgtcc tgcgcggcga
    30721 cgcggcgatc gaagggggct acggggagtt cctccgccgc tccgcgcaga gcgagtcgcc
    30781 cgtcgtcgcg gcggtctccg gcgagcggct cctcgggatg tggcgcgtcc ggccggaggc
    30841 gctgaagaag ctggagatcc cccgcgagcc ggcggacgag agctggacgc gcgagaaggg
    30901 gcacgctccg ctcgacgtcg ggctggacct cgccccgctc gactcccttt cccgctggag
    30961 cgccctcgac cgtggagacg gcgccgtcgc gctctccccg cgcgccgccg tttcgaaagc
    31021 ggcccgtctc tccggcggct cgaccgtcgc cgaggaggcc gccgtcctcg ggaaggcgac
    31081 gctcgacggc gtctcggtcc tcccacgctc cgtcattccc gacggcgtct ccctccgcgg
    31141 cgtcgcggtc tcgcagaacc tcgtcgtcga cccggtcgcg ggcgcggcgt cgctcctgac
    31201 ggacctcctc ccgcccgccg gtccggcgcg cggcgccggg atcgggagcc gcctcgccgg
    31261 cctcgtcctc ttcctccttt cgctgccgct ctggccgatc gcgtacgcat gggccttcgt
    31321 cgcgaacgcc ggacgcccga cgcgcccctg ggccttcgcc ggaaacggcg cggagccggg
    31381 gacgcggcgc acggtgaaga gcttccggtt cgagacggcg atccccgtct tccgggacct
    31441 cccgcttctc ctcgccctcc tcgggggctc gctcgccctc gcgggggtcg cgcccctcgc
    31501 accggaggag gaggagacgg cggcgcggga cgcctgggtc ctggcccggc gcgaagcgcc
    31561 ggtggggctc ctcgcgcgtt cccggatggc cgtccccgcg tcggctcccg gcgaggtggc
    31621 ccgcgtcgtc gacgcgttcg acgcgcgtcg cggctgtcgg gggctcgtcc cgctcggact
    31681 ctcgactttc ctttcggcgc gtgcctggac cgcgccgaag ggctggaacc ccgacgagct
    31741 cccggagtcg cagggctgac ccgccgcaag gagacgacga tgtccgacga gcaccgttac
    31801 accgcgtccg ccgcctggga cccggccgag cgctccggcg ccctcgaaag cgggagccgc
    31861 ctcctggccg tcccgttcgg cggggccccg tcgctcggcg gacgggacga ccggacgaac
    31921 ccggaggagc tcctcctcgg cgccgtcctc acctgcttcg tccagacctg ggcgatcttc
    31981 ctcgcgaagc tccggctccc gctcgactcc ccgcgcgtcg acggcgctct cgacctcgag
    32041 gccgacccgg cgggaggcta ccgcgtccgc cggatcgacc tcgtcgcgca cgtcccggcc
    32101 gccctcctcg ccgagcgcga ggccgacgtc gcgaagtcgc tcgccctcgc cgagaagtac
    32161 tgcatcgtct cgaaggcggt gaagggcgag gggcgcgagg tccacgtcac gactcgtccg
    32221 gcctgacgcg cctcccgctt ccgaccccat aggaggggag gcggcgcagc cgccgcgggg
    32281 aggctcgtgg cgagtctccc cgatccatgt ccgcaagaaa gcccgaagag tcggaggcgt
    32341 tggccggcgg aacgggcgtt cccgctccac ggacctcgat tccccatcaa agcgccgaag
    32401 ccgccgcggg gaggctcgtg gcgagcctcc ccgatccatg tccgtaagaa agcccgaaga
    32461 ctgggggacg tcgaagggag ccgcagacct gcccctccaa cagacgtcta gtttcttcgg
    32521 taatgacacc cgcgcgagac ggggttcttc agcgccgcct gcgcgatcag gtggctcgcg
    32581 tggacgccgt ggaagaggtc gacgatctcg cgggagagct tcgtctcgtg gtagaagcgc
    32641 gagagacgct tctccaggtc gcggatgtcg gccacggcgc gcccgagccg ctcgcccgtc
    32701 ctcacgatcc cgacgtagtt ccacatcgtg ttgcggatcg tcgtccagtc ctgctggatg
    32761 agcgcggggt cctcgttccg ggcgttgccg ggcgccttcc agtcggggat cgcgcggaac
    32821 gtcgacggcg ggaacgtgtg tcccttctcg atccggtcgg cgatcgaccg ggccgcgtgc
    32881 gccccccaga cgagcccctc gaggagcgag gtggaggcga gccggttcgc gccgtgcagg
    32941 ccggtgcagc tcacctcgcc gacggcgtag aggttccgga tcgtcgtctt cccctcgagg
    33001 tcggcgagga cgccgccgca gaagtagtgc gcggccggga cgacggggat cggctcggtg
    33061 gcgatgtcga tcccgaactt ctcgcaggtg gcggcgatcg tcgggaaccg gtctcggatc
    33121 gccgtccgtc ccttcccctc gtagtggtgc gcgaggtcga ggtagacgca cggctcgccg
    33181 cgcgtcgtca tctcctccac gatcgcccgg gtgacgacgt cgcgcggggc gaggtccttc
    33241 cgctcgcggt cgtagcgcgg catgaaggcc tggccggagc ggttgacgag gaccgccccc
    33301 tcgccgcgga gggcctcgga gatcaggaag cgctcggcgt cggggtggta gagcgtcgtc
    33361 gggtggaact ggacgtactc gaggttcagg acggccgccc cggcgcgcgc ggccatcgtc
    33421 aggccgtcgc cgatggcgtg tctcgtgttc gtcgtgtgaa ggaagagccg cccgacgccc
    33481 ccggtcgcga ggacggtgaa gtcggcgagg acggtgtgga ccgccccctc ctcgtcgagg
    33541 acgtaggcgc cgaggcacgg gtcctggagc gcgtagcgct gctggacgtc ggtggagtgg
    33601 tggcgggcgg tgatcaggtc gatcgcggtc gcgttcgtcc agaccttcac gttccgctcg
    33661 cgcttcacgc gggcgaggag ggcgagctcg atcgccttcc cggtggcgtc cgcggagtgg
    33721 acgatgcggg cgacggagtg ggccccctcg cgggtgaagt cgaggtctcc cctccgggtg
    33781 cgggtgaagg ggaccccggc gtcgccgatc aggacctcct ccacgacgcg cggcccctcc
    33841 tgcgcgagga agcggaccgc ctcggcgttg cagaggccgg cgccggcggc gaggacgtcc
    33901 ctcacgagcg aggtggggga gtcgcgcttg ccgaagtaga tgattccgcc ctgggcgtgg
    33961 cgcgtgttgc agtcgtccgg ctccgcggcc ttcgtcagga ggaggacgcg gatgccccgc
    34021 ttcgcgagcg cgagcgcggc ggtcagcccg gcgatcccgg tcccgatgac gagcgccggg
    34081 acgcgggtcc gcccggcgac ccggctcacc ggcggccccg ccgggccgcg gccaccttca
    34141 cgtcgagggc gacgtcggcg gcgcgcaccg agtgggtgat cgccccgacg gagacgaagt
    34201 cgacgcccgc gcgggcggcc ttccgggcct tctcgaccgt catcccgccc gaggcctccg
    34261 tcgtcaccgt ggcgcccgtc gcctcgttcc agagcttcac gtcgcggacc gcttccctca
    34321 tcgcggcgat ggtcatgttg tcgaggagga ccctctcggc ccccgcctcg agcgcctggt
    34381 ggacctcgtc gcgcgtgcgc gcctcggcct cgaggaggca gcggcggagg gagcggttca
    34441 tcgcgcggaa gtcggccgcg cggcgcgtgg cgtcccagag gctcccgcag ccgtcggcgt
    34501 ggttgtcctt cacgaggaag gcgtcggaga ggcccatccg gtggttcgtc ccgccgccgc
    34561 atcggacggc gtacttctcg agggcgcgca ggcccggcgt cgtcttgcgc gtgtcgagga
    34621 gcttcgcctt cgtcccggcg agggcgtcga cgtaggcgcg cgtcgccgtc gcgacgccgg
    34681 tgaggcgctg gacgaggttc agcgcggtcc gctcggccgc gaggaccgag atggccggcc
    34741 cctcgacgtg cgcgaggacg gtgccgggcg cgaccttcgt cccgtcctcg acccgcagct
    34801 cgacgcggga gggagggtgg aggaacgcga agacgcgcgg gaggaaggcg acgccggcga
    34861 cgacggccgc gtcgcggcag accatgtcgg cctcgacgcg ggccttcggg ccgaagacgg
    34921 cgagggtggt gacgtcctcc ccgtcttccg cgaacgcgcg aacgaggagg tcgtccaggg
    34981 cttcgacgag gcgtcgatcg gtcttcacgg gaacagcatt ctagacggcc cggcgggtgc
    35041 gcgccgcggg gcccttcgcg taggattccc gccgatggag gactcgatga tggccggacc
    35101 cgtgatcccg gaacggtacc gagcggcgct cggggacgac gaccccttcc tcgcgatgtt
    35161 cgaggcgccg caggcgctcc ggaagctcct gaaggggctc accgagaagc agctctcccg
    35221 acgtcccgcg ccggggaagt ggtcgatcaa ggagatcgtc gcccacctcg ccgacggcga
    35281 ggtgatcctc gggtcgcgct accggctcgt cgcggcgcac gagaggcccg cgatcgccgc
    35341 ctacgaccag gacctcttcg tcgagaagct gggcgcggac gccgcgtcga cggaagagct
    35401 cgtcgccgac ttcgagatgg cgcgcgcggt gaacctcggg ctcctcgtga ggctcccgga
    35461 cgaggccttc gcccgggtcg ggctccactc cgagagggga gaggagtcgc tcgggacgat
    35521 ggtcgcgctg tatgccgggc acgaccggat ccatctcgac cagatcgaga cgatccggac
    35581 gggcctcttc cccgcgaaga agaaggcgcg gaaggccgcc gcgaaggcgg ccccgaagcg
    35641 cgcggcggga aaggcggcga ccccgaagcg gtccgcgccg aagcccgggg cgaaggcgcc
    35701 ggtgaagaag gccggccgga aggccgcgaa gaggagctag ggggcggcgt caggcggggc
    35761 cgcgcgtgag gcgggccgcg aggcccgcca gcggtcccgc cacggggacg acgagccggg
    35821 tgacggcgcc cccgaggaag acggggcgga agaggcgcga gaagaagtag acgcgccccg
    35881 tcgcgtcgcg ccagcgtcgc ccccactccg ctccgagcgc cggggagggg acctctccgg
    35941 cggcgtggcg cgcgaggagc gtccccgcgg attcgccggt ccgaagcgcg agcgccatcc
    36001 ccgcccccgt gtaggggtcg acgaccccgg ccgcgtcgcc cgcgaggagg atctctccga
    36061 gaacggcctg ccgggccccg aggtggaccg gctcactcac gagcgcgggc cccggctcgg
    36121 gcgcgagccc ctcgaggtct gcggcgaggg ccgggctctc ggcgcggagc cgcgcgagca
    36181 ggcgctcgag gtcgtggtgc gcttccttcg cgaccgccgg cgtcgcgagc gctccgagcg
    36241 agacgcgccc ctcctcgacg agggagaggc ccaggtagcc cccgcggaag aggtgcaggg
    36301 cgacgcggtc gtcgagcctc cggcccgcgt ccccggcgag gaacgtcttg aagccgaaga
    36361 gcggcgcggg cgcgccgaag aacggtcgcc cgaggcggcc gtcgagcggc gagtagcgac
    36421 cccacgcgcc gaggacggcg cgcgcggaga gcgtccgcgc ccccgtcctc acggtgaagc
    36481 ccgcgtcgag gctcccctcg atcgcggtga cggcttcgcc gaggaacgcc gcggcccccg
    36541 ccgcgcgcgc gccgtcgagg acgaggacgt cgagccgctc ccggctgagg gagagcgccg
    36601 gcgccggaag acgtccggcg aggcgtctgc cgtccggtcg gacgaggacg aaccggcgga
    36661 ccgtcgcggc gccggccgcc cggaccgctc tctcgaggcc gaggcgggaa agggcggaga
    36721 cgccgtcggg ggagaggagg tccccgcacg ccttcgggcg cgggaacggc gccctgtcga
    36781 cgattgcgac gttcgctccg gagcgcgcga ggacgagcgc ggcggcgctt ccggcgaggc
    36841 tcgctccgac gacgaggacg tcgaaaggcg gcgcggtcat ctccgggtcc agacgaggcc
    36901 ctggacgaac ggacccgcgg ccgcgacgcg tgcttcgggg agggcgccgg ccacgatttc
    36961 ccgcagctcc gccttcgtcc aggcctggag gacggaggcc cggccgtcgt gccgcgtgac
    37021 cgggctccgg taggcgaagc cggacaggag cgcgtggacc gcgagcgggg cccggtggcg
    37081 gacgaggtcg aaagcggcga ccgcgacgcg cgcgacgcgc gcggactccc ggaggagggt
    37141 ggccactccg tgggcgtcga ggtggtggag gaagagggtc gaggcgacga cgtcgacgga
    37201 gtcgtcggcg agcgggaggc ggagcgcgtc cgcggcgagg ctgcgcccct tcgccgagag
    37261 ccgcgcgtga aggagcttcc tgtcgaggtc gatcgtgacg agaccgcgcc cgtgccgcgc
    37321 catctcccgc gagagcgccc gtccgacatg gccattcccc gcgcccaggt cgaggacgac
    37381 cgggtccgga cgtggcgcgg cgaggaggag cgggacgagg tgccggcgca gggtccgccg
    37441 gccgccgaag aggcggtgga tccgctcaat gtcgccgagg ctcgccacgg cctcggccgg
    37501 gtcgacgtcg ccgtcgagaa gctcgtccga cggcgcgcgg ggcgggacga ggaggctcat
    37561 gcgcgtcatc ccgggagcgc gcgggcggta cggacgacga gggcgctgtt cttcgagccg
    37621 aacgcgatcc cgttcacgag cgcgacgccg acttcggccg gccgggaatc gcgcgggacg
    37681 tagtcgaggt cgcattccgg gccgggcgtg tcgaggttcg ccgtcggcgg gaggaacccg
    37741 gcgttcagcg cgaggagcgt cgccgcgagg cccgcggcgc cggaggctcc ctgcgggtgg
    37801 cctatctggc tcttgacgct gctccccggg accctctcgg ccgccgcgcc gagcgccagc
    37861 cgcgtcgcgc gcgtctcgat ccggtcgttc agctccgtcc cggtgccgtg gtactggacg
    37921 tagccgacgt cccggacgtc gaggccggcg tccccgaggg cctcggtcat cgcgcgcgcc
    37981 ggctcgacgc ccgactcgtc cagccgcacg cggtgatacg cgtcgcacgt cgagccccag
    38041 ccggcgacct cgccgaggag gcgggcgccg cgagcgcggg cgcgctccat ctcttccagg
    38101 acgaagaacc aggacccctc gccgaggacg aagccgtcgc gcccggcgtc gaacggccgg
    38161 ctcgcggtct ccggcgcctc ctgccgcgag gaggcgagga tctgcatcaa gccgaagccg
    38221 gtcatgatgc cgggggtgat gacggcgtcg gctccgccgg cgaggacgac gtcccgctct
    38281 ccgtaccgga tcgccttcgc cgcgtagccg atcgcgtcgg cggaggaggt gcagccggtg
    38341 gagacgacgt gcgagggccc cttcaggccg aaccgcatcg agacttccga ggagagcgtc
    38401 ccgatcgtcc cggtcgggat ggcgtagacg gaagccttct tcagctcgcc gcgataccag
    38461 ttgccgtaca tccgctcgaa gaactcgacc ggacccccgc cggagccgag gaggacggcg
    38521 atccggcgcc gctcgtcgag cgggagggcc tccacctcga tgccggcgga ggcgagcgcc
    38581 tcggcggccg ccgcgatcgc catcgggacg acccgcgaga cgtgccgggc gtccttcgcc
    38641 ccgacgtggt tcgccgggtc gaagccggac gcctccgccg cgatccggac cgggaggtcc
    38701 gacgggtcga acagcgagat gcgccggacg cccgaccggc ccgaccggag gccgtcgagg
    38761 aacgccccgg cgcccgtgcc gagcggcgtg acgcagccga ggccggtgac ggcgacgcgc
    38821 ctcctcatcg gccggcgggc gcagcgcgcc cggcgccccc gcccggaggc tcggccgtct
    38881 gccgtcggga atccatcgac gggatcgtag cagccggttc ggggcgggac ttccgtacaa
    38941 tccggggggt gtcggacagc cgcttcgagt tccgcggcga cctctcgaaa actccgcttc
    39001 ccgaggtcct ccagacggtg caccactaca gggtgcccgg ggtggtcgtc gtgcgccggg
    39061 gggtggtcga gaagaagatc tacatctgga acggcgacgt catcttcgcg acctcgaacg
    39121 accgggagga gtccctcggg aacgtcctgc tcggggacgg aaagctgacg caggcgcagt
    39181 tcgacgagtc ggtccggcgg ctcgtcgagg cgcgttcgac cgaggggggg cggcggcacg
    39241 gcgccgtcct cgtcgacatg ggtctcctga cggccgagga gctcttcgcg gcggtgacgc
    39301 gccaggtccg cgggatcctc ttctcgctct tcgagtggga cgaaggcgag gtgaccttcg
    39361 cggtgggccg attccggacc gacgagatca tccagctcga gatcccgacg cggcaggcga
    39421 tcctcgccgg cgtgcgggct ttgcgcgacg cccggaagac cgtctcccac ctcggaccgt
    39481 cctggacgat tctcgacccg tcctggaagc cggcggatct cggcgacctc gccctcgagc
    39541 cgggagagct ccggctcctc cagcaggtcg acggcgtgaa aacgctccgc gagctcgtcg
    39601 ggctcgggcc gtccgacgcg gcccacaacg cgaagctcct gtacgccttc tacgtcctca
    39661 agctgatcgg ccggcgcgac gtgacgagcc ggacgtccgt gaagaagatc cactggagca
    39721 cgacgggcgg cggctacgcc ccgccgtcgt gagagaaagg tccgtttcga gatgcccctc
    39781 tacgagttcc ggtgcgagtc gtgcggtcac acgatggaga cgatcctcgc gttctccgat
    39841 cctctcccca cgacctgcga gacgtgcggc ggcgcgctga agaagctcct ctcggccccc
    39901 gccgtccagt tcaagggctc cggcttctac ctcaccgact acggacggtc cggcgccgcc
    39961 ggcgcgaagg agaaggagtc gggaggtgac ggcggcgcga agccttccgg ggagccgaag
    40021 ccctccgccg agacgaaggc gggcggcgac gcgaagccca ccgcggcggc gagcgggtcc
    40081 ggcacggggg gcgatgcgaa gccggcgggc tgacctcgcg gccgctctag tcgcggtcgc
    40141 cctggccccg gctttcgcgg cggccgacgg ccggccggtc acggcgccgg gagggacgtg
    40201 gctgcgctat ccgctcccgg gagccgaggt gaagtcgctc gtggcggagc cgcgcgcgcc
    40261 ggggctcttc tacgccggga ccgccctcgg cggcgtctac cgttcgaccg acggtggccg
    40321 gacctggagc gcgccgccgg gaggcgcccc cttccccggc tattccgtca cctcgctgct
    40381 tcctgatccg cagcgcgtcg ggacggtctg ggccggcctg acgggcgtcg tcaagggggg
    40441 gctcctcgtc cgctcggacg acgccgcccg cacctggagc gtcgtgagac ggtgggcgga
    40501 gcgggccgag gcccgcgtcg tcgcggtcgc cgtcctgcgg ggacggaagg tcgtcgcggt
    40561 cggcggcgac acggggctcg agatctcgga ggacggcggc gccacgtggc ggccgtccca
    40621 gcccccgctc gacccgggca gcggcgtggc gttcctcgcg ttccacccgc tcaaggtcgg
    40681 cgtcctctac tcgggctcgt accggcaccc cttccgctcg accgacctgg gccggacctg
    40741 gaagcggatc gcggggggga tggtcgagga cacgcaggtc ttccacctcg acttctcgcc
    40801 gaccgacccc gacgacatct gggcggcgac gtgcggctgg gtctaccgga cgacggacgg
    40861 cgccgcgagc tggacgaggt acaaggacgg tctcgctgac cgaaggaccc acgtcgtcgt
    40921 ccgggacccg cgcgacccgg cgcgcgtcct cgccgggacg accggcgggc tcttcgagag
    40981 ccgggacgcg gggaggagct tccggcgcat ctcgcgggac tcgaccgtcg tcaacgcgct
    41041 cgtcttcgac ccggcgaacc cggcggcgct cctcgtcgcg accgaggcgg agggaatcct
    41101 ccggagcgag gacggtgggc tgacgctcct cgagtcgaac gcgggcctct cggaggcgcg
    41161 ggtctcggcc gtcgccgtca ccgcgtcggg gcgagtcgcc gtcgcgcggg ccgcggacgg
    41221 cgggagcggc ggtctctggg acctcgaccc cgcgacgggg aaggccgaga ggatcgcggc
    41281 ggcgccggcc gcgacgatcc gcgccctcct cgcgagcggc gagcggctgc tcgccgggac
    41341 gccggacggc gtctggctcg cggcggctcc ggacgcgccg ttcgcgcgcg tcctcggtgt
    41401 cgcggtccac ggtttcgcgg ccggtccgga ccgcctcttc gccgcgacgg acggaggggt
    41461 gttcgagtcg cgcgacggcg gcgcgtcgtg gggccgcgtc ggcgacctcc ggtcccgcgt
    41521 ggacaccgtg atgtggagcc ggtcgccggg gacgggagcc gagacgctcg ccgtccgctc
    41581 cgcgggccgg acgctctggt ggaacgggcg ggacttcggc ctcgacgcga ggagcggcgc
    41641 gaaccctcgc ccgctctcgg gtggcttcgg ccgcccacgc gcctcggcct accggccggc
    41701 cgagccgatc gggctcgacg tcgactccga gaagggacgt ctcctctacc gcgtcccggg
    41761 acccggcgcc gacgtcgccc tcgcgctccc ggagcggggc ctccacgtcg ccggctgggc
    41821 gggggacccg cgcagcgcgc ggggactcta cctcgcgacg atcgggcgag ggctcttccg
    41881 cttcgtcccg tccgcggcgg gaggttccgc ctcaggcgag ctcagcgccc cggcacgctg
    41941 aggaacgaga ggcgcgcgcc cgggacgacc ccatggcgcc cggcctctcc gcccgcgagc
    42001 tcgaccaccg tgtcggccgg cgcgcgcgcg ggatagctcg ggcacgggtc ggcctcgcac
    42061 ggggggacgt tctccaggac gtcgacgacg gtcccgtccg cgagcgcgaa gaccatgtcg
    42121 agcgggaagt gacagttctt catccagaac gagcgcggct ccggcgcgtc gaagaggaag
    42181 accatcccgc tcgacggcgc gagcgactcc cggaacatga gcccctgcgc gatctcctcc
    42241 ggcgtccggg cgagctcgag ggagaagacg gcgcccgacg ggagggcgac ccgcggcccg
    42301 gccgcggggg tcggcgcggc cggcggagcg ccgcgacccc cggagcaggc tgcgaggagg
    42361 aggacgccga gcaggacggc cctcccgggg tcacgtctcg tcgacgaccg ggcccggttc
    42421 gtcatacggc tctccttcgt cgtcatgtcc gatctggtac ttcttggcgt aatagcggaa
    42481 cgaacgctcc gacatcccga cgagcttcgc ggcctccttg cgaacgccgt tcgcccgctc
    42541 gagcgcctgc tgcatgaggc gcttgccgat ccactcgagg taggcctcga ggtcgaaggc
    42601 ggtcatcggc aggtcgaagg gagtctggag cgcctcggcc gggacgcccg cgggccgaag
    42661 gaagctctcc gggagcgacg tcgtcgtgat gaccggcccc ggctcgaggg cgacggtcct
    42721 ctcgatcagg ttctcgagct cgcgtacgtt ccccggccag gagtaggcct cgaggagccg
    42781 gatcgcctcc gacgagatct tcttctcggg gatcttctgc tcggtcgcca ccttcttcag
    42841 gaagtggcgg acgaggagcg ggatgtcctc gcgccgctcg cgcagcggcg ggatctggag
    42901 cgggatgacg gcgatccggt agtagaagtc ctcgcggaac gagcggtccg cgaccttctc
    42961 ggcgaggtcc ttgttcgtcg cgcagatgat cctcacgtcg atcggctcct cgacgttccc
    43021 gccgacgcgg cgcacgaccc gctcctggag gacctgcagg agcttgacct gcatcgagag
    43081 ggaggtctcc ccgatctcgt cgaggaagag cgtcccccct tcggcctcct tgaagagccc
    43141 cttcttctcc cgcacggcgc ccgtgaaggc ccccttctcg tgcccgaaca gctccgactc
    43201 gaggaggttc tccggaaggg cgccgcagtt gatcgacacg aacgggcggc cggcccgggc
    43261 cgagctccgg tggatcgccc gggcgatgag ccccttgccc gttccggagt cgccggtgat
    43321 caggacggtc gaggtcgtgc gcccgacgcg gtcgaccagg tcgaagacgg cgcgcatccg
    43381 ccgggagacg ccgacgagcg ggccgagcgc cggacggtct tcgacgcggg agcggagctc
    43441 ctggttctcg tcggcgaggc gctttcgctc gagcgccttc ccgacgacgt gcttgagctc
    43501 gtcgacgtcg aacggcttcg agacgtagtc gtaggcgccc gccttgagcg cctcgatggc
    43561 ggtcttcgtc gaggtgtacg ccgtgatcat cacgaccggc gtctcggcgt ccgtctcccg
    43621 cgtcttccgc aggacgtcga acccggtgcc gtccggcatc ctcaggtccg tcaggacgag
    43681 gtcggggtgg agctcgccga cgaggcgcag cccctcggcg caggagccgg cggtgcgcac
    43741 gtcgtacccc tcccgctcga agacgatcga gagcatcccg gtgatgccgg gctcgtcgtc
    43801 gacgacgagg atgcgggggc ccgccggccg gggcggcgcg cccgtcatgc ccgcaccccc
    43861 atcgcctcgg tcgtccgggc gcccggtcct ccgacgggga gagtgagcgt gatggcggtt
    43921 ccgtcgcccg gggcgctctt cactcggatg ctccctccgt gctcctcgac gatacggtag
    43981 acgatggcga gcccgaggcc ggtcccgccc ggagtcgcat gggcgaacgg cgtgaagagg
    44041 cgctcctgat cttcccgcga catcccccgc ccgtcgtccg agaacgtggc gctccacact
    44101 cccccgccgg cccgcgcggt caccgcgagc gtccccccgt ccggcatcgc ggcgacggcg
    44161 ttccggagga ggttccagaa gacctggcgg agctgtccgg ggtcggcctg cagcgggaac
    44221 gactcgggct cgagcttcaa cgtcacggca tgccgttccg agagctcgtc gctgtgcgtc
    44281 aggagcgtga cgacgtcgcg aagggcgccg gcggcgtcga ccgtctcgat cgcctggtcg
    44341 cgcggcttca cgtacttgag gaagtcctcg aggatggcgg agagccggtg cgactcctgg
    44401 accgcgatct ccatcagccg ccgctccgag gagccgggcg gcgccgtcgc ggacagggcc
    44461 tgtaccgagc cggagatcga ggccagcggg ttgcggatct cgtgggcgat cgacgccgcg
    44521 agctctccga cggccgcgag cttctccttg aggcggacct ccccctcgag cttcttcatc
    44581 tcggtgaggt tctggaagat gaggatccgg ccggtcacgc ggccgtcgcc gtcgcgaagc
    44641 ggcgtgatgg tcactccgaa gtaggccccc gcgccgagcg gcgtccggtt cccctcgaag
    44701 cggaagacct cgttccccgg agggtgggtg gggtccagga tctcgggcca gacgaggccg
    44761 gtctcgaaaa gggggcgtcc gacgagctcc gcgggattcc ggtggaggag ctctccggcc
    44821 ggcgggttcg cgaacgtgat ccggccggcg aggtcggcgg tcacgacgcc ggaggacatg
    44881 gaggagagga ccgaggagtg gagggcctgg agccgcgcga cctcctcccg ccggcgctcg
    44941 gcgaggagcc
//
LOCUS       DAOONL010000020        43521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_628_length_43521_cov_21.747366, whole genome shotgun sequence.
ACCESSION   DAOONL010000020 DAOONL010000000
VERSION     DAOONL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 43521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_43521_cov_21.747366"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..740)
                     /locus_tag="PLP50_05150"
     CDS             complement(39..740)
                     /locus_tag="PLP50_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50972.1"
                     /translation="MTTSDYAWTRFLVPRDGRLTLADDGFLADPEGLFGRAANADLQR
                     LEDVMGFPCLVLLGERGIGKSRTLKALAASEPGPERSAVVDIGGVASDEALEAKLKRN
                     PDYVAWKGCGGKLFLYLDSLDECFVRAGSLKRNILELIGDLPNDTLRLRIACRTGHWP
                     ATLETGLEGALKSPPGIFEIAPLRRKDVLLAATTERLDGGSFIAAVEVAHAAPFAANP
                     VTLSMLLGLFRGVSR"
     gene            903..2306
                     /locus_tag="PLP50_05155"
     CDS             903..2306
                     /locus_tag="PLP50_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HPA50973.1"
                     /translation="MLLTLSTTHRPASDLGYLLAKHPAKFQSFELAFGSAHVFYTEAS
                     EERCEVALLLEVDPVGLVRGRTAGAEGLLDQYVNDRPYAASSFLSVAIAQVFGSALGG
                     RSKGRPELAETAIPLEARLPVLPCRGGEDFLRRLFEPLGYEVEARRLPLDDAFPEWGE
                     SPYVDVTLRRTATLREFLTHLYVLVPVLDDEKHYYVGTAEVEKLLRFGGGWLASHPER
                     EAITRRYLKHSFRLTREALARLVSDEAEDPDADEGRRDREEEAVEERISLNQERMGTV
                     LAALKGLGARRVLDLGCGDGKLLARLVDERAFEEIVGVDVSCRALEVAARRLHLDRLP
                     ERQRSRIRLLQSALNYRDARLAGFDAAAVVEVIEHLEPGRLDAFARSLFEFAKPGAVV
                     LTTPNAEYNVRFEGLPAGKLRHRDHRFEWTRAEFEGWCRGKAETFGYSVRFAPVGPVD
                     QQVGAPTQMAVFTRAEA"
     gene            2303..4837
                     /locus_tag="PLP50_05160"
     CDS             2303..4837
                     /locus_tag="PLP50_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HPA50974.1"
                     /translation="MKISIPNLSLVVLVGPSGSGKSSFARKHFLPTEVLSSDFCRGLV
                     ADDESDQGATNDAFEVLHFVARKRLAAGRLTVIDATNVQPEARKPLVALAREFHCLPV
                     AIVLNLPEKLCHERNASRADRAFGPHVVRGQVRTLRQSLRGLGREGFRHVFVLSTPEE
                     VEEASVERVPLYNDRRGEMGPFDVIGDVHGCSAELRALLARLGYGEGGHPDGRKAVFL
                     GDLVDRGPDTPGVLRLVMGMVAAGTALCVPGNHDMKLKRKLAGKDVRLTHGLAESISQ
                     LEKEPPEFVDTVSAFLDSLVSHYVLDGGRLVVAHAGMRADMQGRGSGRVRDFALFGET
                     TGETDEYGLPVRFNWASEYRGAAMVVYGHTPVAEPEWLNRTINVDTGCVFGGSLTALR
                     YPEKELVSVKAERTWYEPARPFLPEEAQAPALTTQHALDDMLDLEDVTGKRLVTTRLH
                     RSVTIREENAAAALEVMSRFGANPKWLVYLPPTMSPAETSEREGLLEHPAEAFAYFRR
                     EGVPSVVCEEKHMGSRAVLVVCRDEEVARKRFGVVGEGIGACVTRTGRRFFEDEGLEQ
                     ELLARVAGAIDRAGLWEELKSDWMVLDCELMPWSVKAQQLLRSQYAPVGAAANAALPL
                     AASLLERSLGRDEAYGAVAERVAARAGLARRFVSAYRHYCWRVEGLAGLKLAPFHLLA
                     TEAGVHVDRNHLWHMETLRRIAEADSGVVIATPYRVVDVTDPASEAEGISWWEELTGR
                     GGEGMVVKPLDFLARGARGLIQPALKCRGPEYLRIIYGPEYTLPANLERLRKRGLGAK
                     RSLALREFALGVEGLERFVRREPLRRVHECAFAVLALESEPVDPRL"
     gene            complement(4883..5752)
                     /locus_tag="PLP50_05165"
     CDS             complement(4883..5752)
                     /locus_tag="PLP50_05165"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPA50975.1"
                     /translation="MTPLQKRLLALTRSLGVPLTTLEKDVALGALLAAIAADSRLSGT
                     LVFKGGTALKRLYFGDTYRFSEDLDFSALAAPRGADLAAALDAARIRAVTALSAEGPF
                     DARLEPLREREAHPGGQDAFVVRFRFPWQRDALCPIKVEVTHDEPVLLPPATRRILPG
                     SGLAPAGSLLAYPLEEVVAEKLRALLQTDARLAVRGWSRPRARDYFDLWKILTTFAAE
                     VDPATVRRLLPAKCALREVAYTDTASFFTERLIREAEKHWVEALADLVPDLPPIEQVL
                     AELPPLVSRLVGE"
     gene            complement(5749..6606)
                     /locus_tag="PLP50_05170"
     CDS             complement(5749..6606)
                     /locus_tag="PLP50_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPA50976.1"
                     /translation="MNARPPDPVRYAEPKGIALIGALIEAKRFVFSAADARNASAKVG
                     LAPGAVETTLRRLADAGWVERLRRGLYATTGELPGLAALHPFAIATAIVQPSAVSHWS
                     ALNHHGLTTQVPRVVTCMTTRKVVTPSMRSGAGRRGPGRHRWETAGGLRASYSTVTPA
                     HYFGIEDVWIDQRSRVPITDRERTALETFARPGAFGGIGEGLGLLEEHLAELDLGRLV
                     AYALRYGATSVAKRLGFALERAGAPVEALEPLRALPATGVRLLDPASPARGRSVRRWG
                     IQDNLTPNR"
     gene            complement(6735..7760)
                     /gene="rtcA"
                     /locus_tag="PLP50_05175"
     CDS             complement(6735..7760)
                     /gene="rtcA"
                     /locus_tag="PLP50_05175"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HPA50977.1"
                     /translation="MTGLVRIDGSMGEGGGQVLRTALALSLTTRRPVEITRIRAGRQK
                     PGLMRQHLTAVQAAAAVGSAHVKGAEIGSRELRFEPGALRHGSFDFSVGTAGSTTLVL
                     QTILPALVLAGGPSTVRLRGGTHNPMAPPFDFLEKAFLPLVGRMGATVDAVLVQPGFY
                     PAGGGECIVTVTPAARLRPLVLLERGEVKRRAARAVVSKLSREIATRELAVVSRLLGW
                     EAERLEAVSVDSSGPGNVVTLEIESAGLTEVFTGFGEKGISAEAVAEKVAREARDYLA
                     SGVPVGEHLADQLLLPMALAGSGAFRTTPLSRHATTNIEVIRAFLPVRFDVEADEKGN
                     CVVRFSG"
     gene            complement(7757..8986)
                     /locus_tag="PLP50_05180"
     CDS             complement(7757..8986)
                     /locus_tag="PLP50_05180"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPA50978.1"
                     /translation="MTDRNFDVIDIPGGVPVKSWTRGVPFEPEAKKQLANIARLPFIH
                     KWVAAMPDVHLGIGATVGSVVPTVGAIIPAAVGVDIGCGMMALKTDLAARDLPDDLHP
                     VRTAIERAVPHGRTDNGGRNDRGAWAEPPDAVAEAWAPLAPRLEALVEKHPAIRKANH
                     VNHLATLGTGNHFIEVCLDEDECVWLMLHSGSRGVGNRIGSYFIELAKEDMRRHFINL
                     PDADLAYLPEGTKHFDDYVAAVGWAQEYAMTNRRLMMANVVAALGALPGLPPFTAGLV
                     AVNCHHNYVEREHHYGKNVFVTRKGAVRARKGDLGVIPGSMGTRSYIVSGLGNPESFH
                     SCSHGAGRSMSRAEARRRFTVADHAKATEGIECRKDEDVIDETPAAYKPIDAVMEAQR
                     DLVEVLHTLRQVVCVKG"
     gene            complement(9458..11020)
                     /locus_tag="PLP50_05185"
     CDS             complement(9458..11020)
                     /locus_tag="PLP50_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HPA50979.1"
                     /translation="MANRRLFRSLVGKLVPATNATNEAGGVAYERTPKQALAQYVATG
                     CLNATFYASADDQLEKLLALAAKVDSEFLAKLAVFARESAWMKDAPALLCAVLSTRSP
                     EHLAKAFPRVVDNGKMLRNFVQVVRSGAVGRKSLGTVPKRLVREWLAGKSGTALFRAS
                     VGNQPSLADVIKMVHPKPADGARQALYGYLIGKEVDETALPEIVRQFEAFKSAGASGT
                     RQVPNVPFEMLTALELGRDEWVAIARKASWQMTRMNLNTFARHGVFEVPGLANLVASR
                     LANPREIARSRVFPYQLLAAHSSAREGVPPVVREALQDAMEVAIAKVPRIEGKVFVCP
                     DVSGSMSSPVTGHRKGATTAVRCIDVAALVSAAIVRANPKAEVLPFEQKVVPVNLNPR
                     DSVVTNARKLAAIGGGGTNCSAALEALNGRRAKGDLVVFVSDNESWADPRTGRGSALM
                     GEWSRFRKRNPGAKLACVDLQPSGTTQAAEGDDVLNVGGFSDEVFNVLSAFAQGKLAA
                     DHWVGRVEEVVL"
     gene            11212..12819
                     /gene="rtcR"
                     /locus_tag="PLP50_05190"
     CDS             11212..12819
                     /gene="rtcR"
                     /locus_tag="PLP50_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HPA50980.1"
                     /translation="MARRKQVVFGLLGATLDRGEGSERWETWRPTVSLFQHEELLLDR
                     LALIHQARFAKLARRLVDDIASISPETETRLHAVELRDPWDFEEVYGALHDFARAYPF
                     DPEREDYFVHITTGTHVVQICLFLLTEARLFPARLIQTSPPERRHSSSPGTYQVIDLD
                     LSRYDRIAARFRKERREGLSFLKSGIATKSERFNGLIERIERVAVASPEPLLLMGPTG
                     AGKSLLARRIYELKKARRQISGPFVDVNCATVRGDGAMSALFGHEKGAFTGAAERRSG
                     LLRAADGGVLFLDEIGELGPDEQAMLLRALEEKRFLPLGSDREVRSEFQLLAGTNRDL
                     QEDVAAGRFREDLFARINLWTFRLPPLRERREDIPPNLDYELDRFAERSGATVRFNRE
                     ARERFLAFAMSGEATWDANFRDLSAAVTRMATLASGGRITVNDVDPEIERLKAQWRRP
                     GARVAPDSADGLVGILGPGASGIDRFDLAQLREVIRVSRESRSLSEAGRTLFAASRAR
                     KSNPNDADRLRKYLARFGLTWAQISGA"
     gene            12946..13635
                     /locus_tag="PLP50_05195"
     CDS             12946..13635
                     /locus_tag="PLP50_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPA50981.1"
                     /translation="MKTIGLLGGMSWESTVPYYVTLNRVVAERLGGLHSARVLLSSVD
                     FAPLEALMRARDWDEIGKTLAREAVRLEEAGARVLVLCTNTMHKVAETLEAAVGIPLV
                     HITDVTGTAVRAAGLRRVGLLGTRFTMEEDFYAARLARLGIEVLTPPADARAFVDRVI
                     FEELCRGQFLASSRSGYAEVMQDLVSRGAEGIVLACTEIGLLLGDAPFPVPLFDTAEI
                     HARAAADAALA"
     gene            13797..14144
                     /locus_tag="PLP50_05200"
     CDS             13797..14144
                     /locus_tag="PLP50_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPA50982.1"
                     /translation="MPQYLVAGYLPDDFDPSQMDEAVGREIHALNEEMIAAGVRKFAC
                     GLGAAKSLRPQSDGGVLVTDGPYLETKEHIGGFWILECADMDEAVAWARKGVAATRGQ
                     VEIREIFFVPTPE"
     gene            14236..14619
                     /locus_tag="PLP50_05205"
     CDS             14236..14619
                     /locus_tag="PLP50_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPA50983.1"
                     /translation="MPQYLIAIQHPEDYDPSLEGEAMIRDIAALNEEMDAAGARFFAG
                     GLESARHARSLRKQPGGGVSITDGPYLEAKEHVGGFWILDCADLDEALAWGRKAVVAC
                     RVPVEVRAFLRIRPKRESPGRGGSA"
     gene            14647..15927
                     /locus_tag="PLP50_05210"
     CDS             14647..15927
                     /locus_tag="PLP50_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA50984.1"
                     /translation="MSGVHETIEAVWRIEATRLIAAIARVVRDVGLAEELAQEALVTA
                     LEVWPEEGIPDRPGAWLMTAAKRRAIDALRRGRMLERKHVELGLELERQQERLGDALD
                     HALDEVIDDDVLRLVFTACHPVLPVEGRIALTLRLIGGLTTAEIARAFLVPEKTLGQR
                     ISRAKKTLAEAHVPFETPRGDELNRRVESVLTVVYIIFNEGYTATAGEEWMRTALSEE
                     ALRLGRILRRLLPGESEVHALIALMELQASRSAARRGPDGEAVLLPDQDRSLWDRAQI
                     QRGFAALERAQRLGGGARPYALQASIAACHMRARTAEETDWGRIVLLYDALLQVLPSP
                     VVALNRAVAVGMAWGPAAGLDELDAATALAGESATASYHLFPSVRGDFLRKLGRLSEA
                     RDEIRRALAMTKNLRERELLEKRLRQWEKATPSA"
     gene            16504..19887
                     /locus_tag="PLP50_05215"
     CDS             16504..19887
                     /locus_tag="PLP50_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50985.1"
                     /translation="MPIRSCSAALSGLLALAMLFVAGPGRADVVWRVADEGPRAPEAV
                     QILGDETRTLAFGPSGDWELRGTTWHAVPLRTTEVVGSERTLFFARGRFGATTRSSSS
                     CRVQLFTLNGDTWTRLWSETSCHPVVRSDERLYVVAEAFRTCGCASDPSLGPEARRLR
                     SVSLADGSVREEAPLPACSGTLFALAGKLHLIGTPPICGGPSSDDVAALVSTPPYDFY
                     RLDEAGWTQLPPWDLPVWTFFESTPNSLWVVHALGGVDYVARILTTTGFSDPVPVPRD
                     ITVSSGGPKPLEWNGRLLATSREGRGNIYLLEGNALVRMTPVSPVTARYPAPLVSVAG
                     NRIFTSADGWDVHERDGSEWIATTGVVGAAGADSYLSGETKTFAVRGSRLYRREDAGW
                     KRLPPSPGAEWAEVIGVWKDRPVVAEWVSNSTSRRHLALDDESEEWVDLKSPEGFDAP
                     LLGVGQDLYLSGPAGRLARLRNGAWAILEASPLPSEQCTSRPVANRRVGGETYAVSTD
                     SCFGPDRRDEAVFRVDGDRLVRAFPNLDRAMRVLDVLEVDGEPVLQVHDASPGVGPRA
                     VLVRPTEAGFDTLLTRDDVQESGRDSLARAEMSSAGSSVFFSGLRFARGRLSRQGGGT
                     VPAKIDPDGRIAYGDRYRFASDARPDPLLTLVPRVRKQIAAVVDATGHGGTRYRSELT
                     VANLSPGAACVAKVYSGAETEPSLEVPLGPGAQKRVADPVPGFVGPLAVEFEGLSDDR
                     EAWAAVRVFSASGGGTAGTSILGSEVGSLAGTTTLLRPIRAPGSRLHLALATSRDGSD
                     VPTWAYADPRQAPEIHASSPLPAGSFLQVDPDPSKLFGPLRIEASTLWLSPIDDARND
                     LLGYFVRNDGATNDGTVVPFEEPDAVPGRRTRFLPAVVGVTSEHGRYRTELTLGRRER
                     WGLPESGLTFTVTYRDEKGASTFPVAVDLDGLVEVPDAGEWLVANGVPIDPNDFAGTL
                     TFSSDRPEGAADLLVTAVVQARAAGAAGDYGVSVPVVNEVRWADGRAVVPALREDAGF
                     RSNVAVANPEPEGGPVVTLEVTLRQASDGSVIGTLPRVTLPPGRRFQWNRPLSQIGYG
                     GDAWAEVRRLGGSGRFVAYGVVNDNVTSDGTLLPMTQRR"
     gene            19985..20908
                     /locus_tag="PLP50_05220"
     CDS             19985..20908
                     /locus_tag="PLP50_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50986.1"
                     /translation="MPLYRAGRDLVRLEAAEPAVRGPAASLWAEVPDTDARADELRLR
                     VEVSGAAVEGDPEEALVWEVSNDETRLVAPGIEMTIRPRERRASATVARSLVGLRPGL
                     VARLLLEAPVTAMKMVTMQLLHAGAVVGPAGAAVVRGAAGAGKSTLVAACRAAGLGFL
                     GDESLLVDRDDADRHEATLRDLVLTPESARLLGLDAASVPAFSGGERKRRVPLPEPLA
                     PADRLARRVATVLLGPREPGPARLVPISGAAFLESFAAGEIPQERMYGGDPAAIARAW
                     VGRETYLLTGAADLGGAVDQIRDLVHRFGAR"
     gene            20932..22407
                     /locus_tag="PLP50_05225"
     CDS             20932..22407
                     /locus_tag="PLP50_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50987.1"
                     /translation="MSAAGPPVLFAHSCFLAHDPKQVRKMRPYPPLATLLAASVVRRA
                     GREVALFDAMLSAGEEEFETALARVAPRVVAILEDNFNFLTKMCTQRSREASVRLIRS
                     AKAAGARVAVNGSDASDDPAFYLDVGADAVILGETERTIVDLLDAWDRSPDADLSQIP
                     GLALPAGAVRAAGSAPTRTPPRPFVADLDALPLPAWDLVDVERYRKAWTEAHGRLSWN
                     MVTTRGCPYRCNWCAKPLYGVRYAQRSPELVAAEMKLLKETVAPDHVWFADDIFGLSH
                     RWTERFAEEVRERDASIPFTIQSRVDLMSPTAVEALRRAGAEEVWMGVESGAQSILDA
                     MDKGTTIAQVRGATRRLKEAGIRASWFLQLGYPGETWAEILATRELVREERPSDVGVS
                     VAYPLPGTVFYERVKSQLGERSHWKDSDDLDMMFEGTYSAPFYKRVRDLLHAEAALGA
                     IPPSETIGRRRRNLDAAWLAAERDEPLSRTAGAALPTRQRG"
     gene            22332..23819
                     /locus_tag="PLP50_05230"
     CDS             22332..23819
                     /locus_tag="PLP50_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50988.1"
                     /translation="MARRRARRAALSHSGRGAPDPAAGLTGSLRGEPMADLLLSHGYF
                     LAEDEKERAVMKPYPPLGLLSLSAYLKREGFSVEVFDTTFATREALFARLDEGPGVLG
                     LYTNLMTRPPVLAITAAAKTRGWTVVLGGPESANYPAEYLAAGADVVVRGEGEETLRE
                     LLPALAARGLHRLDGIAGIAFRDETGVVVSGPPRAQVRSLDALPWPDREAIDHEPYLA
                     AWRERHGEGSVNLITARGCAYKCDWCSHAVFGNTHRRRSPANVAAEVEWIRDRYRPEQ
                     VWYADDVFTISHRWLFEYAAELEKRGLKFPFETISRADRMADEEVLATLARLGCRRIW
                     IGSESGSQRLLDAMRRGVTVEQVRWATHAARRHGIEVGMFLMWGYDGETAEDVAATVE
                     HVRASLPDVYFTTLAYPIMGTGYYEKVKGRVSLSRPWAEGGDRDHVVAGRAGRDTYRL
                     ADRWLRAAVESRRLEETAPEEARALAGEADAARREFLAAFAEGDA"
     gene            23816..24595
                     /locus_tag="PLP50_05235"
     CDS             23816..24595
                     /locus_tag="PLP50_05235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA50989.1"
                     /translation="MIAAARGFDALAATYDEEFTRTDLGRRLRSAVWRHLDAAFAPGA
                     RVLELGCGTGEDAIHLAERGARVVATDASGEMVAETVRKAGAASLSHLVEARRVPFEE
                     LDAAGPSLGTFDGAFSNFGALNCAASLPPVAEALGRLLPSGAPLLLVVMGRWAPWEWA
                     FYLRRGDGARAFRRFRRGGTSWRGLTVRYPSAGRLLRTFAPWFRRRRLAALGVFLPPS
                     YAASSVSPSTLALLDRWERRIESAPLLPRLADHTLFALERR"
     gene            24592..25659
                     /locus_tag="PLP50_05240"
     CDS             24592..25659
                     /locus_tag="PLP50_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50990.1"
                     /translation="MTELAAAARPVPSRAEGALLPAVDPVVSLPIVTLLPFSRCNCRC
                     AMCDIWKERNGAALSRHDVERLLPDLARLGTRRVVLSGGEPLMHPDLFGLVAPLREAG
                     IGLTLLSSGLILERHAAAIAATFDDVVVSLDGPAEVHDAIRGVPGAFERTRRGLRALR
                     GGAPVPALSGRCTVQRENLAHLRATVAAARDLGLDRVSFLAADTTSPAFGRAAGWDEE
                     GRRRVAPDGADLARLAAELDRLELENAADLASGFVSESASKLRERLLAHFRAVGGEAS
                     FPRSSCNAPWVSAVVETDLSVRPCFFHPAYGRIGEGGLAAVLNGEAALLFRRSLDAAA
                     DPVCLRCPCRLNLRDGAGGTP"
     gene            25656..27089
                     /locus_tag="PLP50_05245"
     CDS             25656..27089
                     /locus_tag="PLP50_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50991.1"
                     /translation="MSRRRVVLYNPRAVFFTMPLGLLAVGSHLDRSRYEVVLVDGRLE
                     TDPAAAVLAAIDGEVDGAAPFVLGVSVLTGSPIQDALDVSRAAKARFPGLTVVWGGWH
                     PSLFPEGCLEEPSVDVSVQAQGEETFAEIVRRLDAGESLEGCAGCTHRDEGGAPRREA
                     ARALAPLTDLASHDYGLFPVERFFERKGKRQIDVVTSQGCRFRCAFCADPFVFGRQWV
                     GLPPERVGEELESLHRRFAFTDVNFQDETFFTSLDRVAGIAEELIRRRLPSTWAATMR
                     ADQGDRLPEDAWAACKRSGLRRLLIGVEAGTQEMVDRIRKDIRLEQVLRCAERCLRHG
                     IAAQFPFIVCFPGESDASVEATLDFARRLAAMSPTFQTPVFFFKPYPGTPLTEDAVKH
                     GFRLPATLDEWARFDFYAGDGPFTTPARTRLVERFRWYEKHAYGAAPPWKRPVAALAR
                     WRCERRAFRFPVEKAVGELLFPEPVLS"
     gene            complement(27102..28292)
                     /locus_tag="PLP50_05250"
     CDS             complement(27102..28292)
                     /locus_tag="PLP50_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA50992.1"
                     /translation="MDPHAPPTLLTGARVVGPEGERRADVRLEEGRVSREAIGRAGDL
                     VVPLDGHLLFPGLLNAHEHLGLNAFPEADAGGPRASAYDWIDALQPLLASDSFREVKR
                     VDEALRARHGALKNLLSGVTTVAHHDPWLAEMGDPSFPVRVVHPYGWCHSLRFAGRYG
                     PTVAEALAATPAGARFFVHLAEGTDAEAAGELSRLERLGGLGPATVLVHGVGLGEAGV
                     ARVIERGAAVVWCPASNLRVLGATLDPRALLAVGRLALGSDSRLSGASDLLAELGVAR
                     ESAGLEPEELLRLVTTAAAAVLGRPDAGHLSAGAVADVVVVRDEGGDPAGALTDLPRA
                     RLRAVVLGGVPRVADADLRPWLEAAGVPVRHVLLDGVPKVVDARLFTEEASALEPGLS
                     SAAE"
     gene            complement(28314..28982)
                     /locus_tag="PLP50_05255"
     CDS             complement(28314..28982)
                     /locus_tag="PLP50_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA50993.1"
                     /translation="MPIKVLAPAEAYLRWAPTYEEAGALAELDELAVRRLDPSPAGPL
                     LDAACGTGRRLRSADGAFRRGAFGIDLVLPMLLAGARGAAVAAADLTALPFAAGSFRT
                     AWCRLAIGHVAEAGRVYAELGRALAPGGRLLVTDFHPDAARRGLVRSFRSGGELLAVE
                     HHVHEVDAHRRAAADAGLALETLLEVPAGEEVRARFEAAGKLAAWEAQRDLPVLLALA
                     FRCA"
     gene            29045..30577
                     /locus_tag="PLP50_05260"
     CDS             29045..30577
                     /locus_tag="PLP50_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA50994.1"
                     /translation="MTLPLVLLVNPRVVRRRNARLPLSLLTLAAGLEGKARVRIVDGN
                     FDDDARATVLAALAAERPALLGVTVMPGPQVAPAIALSKAVRERAPGVPIVWGGYFPT
                     LYPDAALAAPYVDVVVRGPGEAALVDLVRAFGSGDRAPALAGIEGISWKDGDHVVHGA
                     ERPLRPPDVLPPLPYELLGDVPRFLQPTVLGQRTAVHQAAIGCRHRCSFCGVVSMFDG
                     KTLVPPAERVARDLLVLKERHGADAVQFYDHNFFDREETTVALAEALAPVGLPYWCYA
                     RTDALARFPARTWELVRRSGLRMVFMGAEAASDEALRRMHKGARVEETLEATRRCREA
                     GIVPELSFVLGGPGGDEAEIETTLRFIRKLKAIHPACEVILYFWTPTPRRRRAECPDL
                     AAEAPAHEPDPGKARAAPDRLDLPATPEEWAEPEWVDFVCHRNAPWLSPRMRRRIDDF
                     ATVLGCRFPTVQDVRLPRWGKAVLSAAASWRWAAGAYGRPAELYALRRVLPLKRPEAE
                     GL"
     gene            30574..31719
                     /locus_tag="PLP50_05265"
     CDS             30574..31719
                     /locus_tag="PLP50_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA50995.1"
                     /translation="MSAEAASPHVVLVNLALVPGLRAGELLARLSGLGRFAASLAEAG
                     ARVSVVARSHEDADLTVGSVRLVTVSDGEGPFAPLRSPARPVVARVRELAPDVVHVGG
                     FLFPVPTATLRAALPRRVPLVLQHHGEPPGSGRLGFLQRLLLRTADAVLITSRLIVDE
                     WVRGGYLAPETHVEEVLEASTDLRPVERAAARSTTGIDGDPAILWVGRLHPRKDPVTA
                     LRAFEAALARLPGARLHLVYGEAPLLPELDALCARSPALARAVRFVGEVPHEQLPAWY
                     SDADLFLTSSPSEGSNWALIEATACGLPAACSDIPANRRVAGPTAAYFPPGDVPSAAE
                     AIVRRSREPGPRGAALRGRFERRLSWSVVAREALGAYERAIVRRGGR"
     gene            31716..32864
                     /locus_tag="PLP50_05270"
     CDS             31716..32864
                     /locus_tag="PLP50_05270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA50996.1"
                     /translation="MRVALLVPGGVDRSLTERVIPALLWLIEELGRRAELVVFALGHG
                     PAPGTWEVPGARVVDLGPLRLSRVPGLAFARRARDLGTLLRAHGPFDAVHAFWATPCG
                     ATALAAAPRLPLVVSLAGGELAGIAEIGYGCDLVPRERRKVRLTLARAAAVTAASRPL
                     VAEALRRGVDARLVPLGVEDAGFLPPAERPAGGPFRLLHAADLNRVKDTPTLLRALRR
                     LLDRGLPVTLDVAGEDTLGGAVQAEAARLGLAETVVFHGRLPTARLRPLLAAADLFLL
                     SSRHEAGPVAVVEAAACAVPTVGTAVGHVAEGDPERSLAVPVGDDAALAEAAASLLLD
                     PGRRHAMGEAARAWARENRASVAAERFAELYREVIDARAGARRKRAAA"
     gene            complement(32818..34017)
                     /locus_tag="PLP50_05275"
     CDS             complement(32818..34017)
                     /locus_tag="PLP50_05275"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPA50997.1"
                     /translation="MSATDEAAPFSSEETMRRLGEPEVAAAGLAAVAALGDGSAAPLA
                     AFAADEARARTAAFAASLQGLGPRLAALPDVTVLPAELRGHLAGEAARCAARGERITG
                     LLARLEELLASAGLRAVPLKGAALVLGKEIPAGLRPMADLDLLFASEADLRAAAAEIV
                     GALGYAPLWNTRRHLVLAEREERVPFPACEHPGNPLRIELHRSFRLEVLGSVLDATDA
                     LRRDAIRAGAWALPSRTALLRHLLFHAAEDFAAKGLRGVQAYDFVALARTSGPLAIPE
                     LRRGERGPVALAARAVERLFPGTFEPGALERAARGVSPRALERAAALPALRHSRPARG
                     WTATALGLADGRLRAARFLLRTLLPSLDEVRANVAPDASGTRLVAAWAGVLTRRLASG
                     ARRLARQ"
     gene            34113..34592
                     /locus_tag="PLP50_05280"
     CDS             34113..34592
                     /locus_tag="PLP50_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA50998.1"
                     /translation="MSPSLPLHPAVIHFPIAAMFFAAAALALAHARPGARAASLSAAA
                     LLLAVAVAGGLAAFVTGWRWADELAYLAGGIGPIPGPKAVEGLAQRHALLGLASVVTA
                     AVALALVVRARRREAPPTLALLAALLASALVGATGHVGGTMVHNPPPPAAESDAGER"
     gene            complement(34614..34943)
                     /locus_tag="PLP50_05285"
     CDS             complement(34614..34943)
                     /locus_tag="PLP50_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPA50999.1"
                     /translation="MSRMTGIAAAVLGTCLLAGPMRAEGPGRSSTWESYCTVCHGSDG
                     KSNTEEGKKRNARNLTDARWLASVSDSRLEGSIRRGRDKMPAFGRKLSDEQVKALVAE
                     VRALAPK"
     gene            35092..35532
                     /locus_tag="PLP50_05290"
     CDS             35092..35532
                     /locus_tag="PLP50_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51000.1"
                     /translation="MSARARLHRLAPLVVFAAVSALACGTRKSPTEPVDDPPNPNATF
                     SRVQAEVFTPSCALSGCHAGAAPAQGMNLSAGSAWAQIVGVPAVGSSRLRVAPGDVAG
                     SYLVSKLRGDATITGSRMPLGGPYLSSDREQLVVDWIRRGAPND"
     gene            35541..36338
                     /locus_tag="PLP50_05295"
     CDS             35541..36338
                     /locus_tag="PLP50_05295"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HPA51001.1"
                     /translation="MKRNALLLALLLLAVPPAAAQEAPAWDPGYGMRFLSTHSPLVND
                     TGILETLFTHRFNQPVNEAGGNDLFGLDNGANIGIGLSWVPVRRVAAEVYRASSGGDY
                     EIAAKVAVLEPTGERPLGIAVRAGASWMTKYDIDRKAGAFGQLLVAWTVADRVTIAAA
                     PSYASNTPLFEDVFNVPLALQVRLGRGLFATGEYVFRNRDLDGSKGQWSFGLEKSVYR
                     HRFLLWIGNSAATTVDQTLAGDYAGGVMESNLRLGFNISRQFRIGGD"
     gene            36367..37242
                     /locus_tag="PLP50_05300"
     CDS             36367..37242
                     /locus_tag="PLP50_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51002.1"
                     /translation="MRPGHGDGEEALGSSRCGNDLLGYFVRNDGATNDGTVVPFEEPD
                     VLPGRRTRFLPAVVGVTSEHGRYRTELTLGRRERWGLPEKGLTFTVTYRDEKGATTFP
                     VAVDIDGIVEVPDAGAWLAANGVPIDPNDFAGTLTFSSDRPEGATDLLVTAAVQARGA
                     DAAGDYGVSVHVVNEVRWAKESALVPGLRQDASFRSNVAVTNPEPEGGPVVTLEVTLH
                     QASDGSVIGVLPQVTLPPGRRFQWNRPLSQIGYGGDAWAEVRRVGGSGRFVAYGVVND
                     NVTSDGTLLPMTAER"
     gene            37472..37798
                     /locus_tag="PLP50_05305"
     CDS             37472..37798
                     /locus_tag="PLP50_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51003.1"
                     /translation="MTRRVLALLVLLVFVAGPALATAEELRHSSARSGPAAVADSCPD
                     PGTDGQPCGPACLCFCCPGHANGLAVFTPRPAVGIPPSSEFELEAHDDLRPKGVLRRI
                     FHPPRA"
     gene            37900..39210
                     /locus_tag="PLP50_05310"
     CDS             37900..39210
                     /locus_tag="PLP50_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA51004.1"
                     /translation="MQPSSLRKFHRWHVHLLWVVLASAGPASAFSDPPTPADAAGAVS
                     ASDSPAVLELTWPDVVRLAEGHPRLAADRLQVAAARSGIAGAGAVPNPTFEADLGRGR
                     ARIGDEARPESELALSIPLGWIAERGPKLEAAVAEADVALAESLASRRELLLQLRTLF
                     WNLAYEQARVGVLETLVAQTAKLTRTVQRRVETGEVRPVEAPLVEIELEKVTAELEAA
                     GTVLRARQSALARWLAVSSDRTVVAKVDLSALPDVPDRETALARARSADPALRVAGAR
                     SRLQSAEVRAERRATIPSFSLRGFVTDELDRKAHGFGISVDVPIWSWNRGRIARAEAK
                     LAAAGRQAEATALEVETTVIEAQAACGASVASARRFRDEIVPRSERVASTMERTYELG
                     EADLLPVIDARRSLLDSRRLSLNALAQAQLDCSRLDALAGEETP"
     gene            39207..40526
                     /locus_tag="PLP50_05315"
     CDS             39207..40526
                     /locus_tag="PLP50_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51005.1"
                     /translation="MNGRILTCILLAAIAPAAVTSCRRAGSAPAGDAHETHSEESHAA
                     NAEPAEPSDLDRPVEELVALTCEHEKKTFECDECRYEAGFVRAPASLVAGGLISTVRA
                     GRQKVAVPVTLTGEVRFDERLVGHVSSPVEGIIRATHVTLGDRVRKGQPLVEIESVAV
                     GEAQAAWLEARSLLSLARRNFERLSALREENISSEKETLQARQELETAEIRAANALGT
                     LTRLGVGSADARTLTSATAGGRIVLRSPMDGLVLTLHAVPGETARTEEALATVGDNST
                     VWVWADLYERDIRSVVRGQASGRLEASVSVKAYPDEVFAGAVDLVTQAMDESSRTVKV
                     RVEVRNRAGHLLSGMFASVRLYLPGADEALAVPGSAVLEDEGRSFVFVHHEGDDYVRR
                     PVTVGRTWAGWAEVTSGLSPGQVVVAEGAFLMKSDVLRSKMGAGCAD"
     gene            40541..>43521
                     /locus_tag="PLP50_05320"
     CDS             40541..>43521
                     /locus_tag="PLP50_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPA51006.1"
                     /translation="MGFVDVLLRSRLLVLLGTALLAALGVVAWTRLPIDAFPDVTNTQ
                     VMILSKAPGLAAVDVEQRVSYPVEQVMRGLPRVQQVRSLSKAGLSQVVIVFEDGVDTY
                     WTRQVVFERLSTAREQLPPGVEPELGPISTGLGEIYQYTLEGDGRSAMDLRTIQDWIV
                     SPLLKPIPGVNEVNSFGGEVKQYQVLVSPEKLLKYDLTIGEVVEAVEQGNANAGGGVL
                     VRGWEQVYLRGVGLLKDVPDIERTVLKAVDGSPVYVRDVADVVIGAEPRQGAVSRDGK
                     GEVVAGMIIMLKGANSQDVVRRVKASVEKVQETLPAGVRLNVFYDRTSLIEACIATVV
                     DALLEGGIFVILVLFLFVAELRTALIVVLSLPLTFLVSFIVMGGAGITSNLMSLGGLA
                     FSVGMVVDASIVVVENVRRHLAHEAAKEHKRRVVAAAVAEVAKPVAFSVIIIAIILVP
                     LFTLQGVEGKMFAPLAATMLIALLVSLVVALTVVPVLSEVFLRQLPEREFAFIRRLRE
                     GYVRLLGHAVRRPAVTLGLSGGALVASLALLPFMGTEFMPPLDEGAIAVNVVRLPNAS
                     LEGSVKVATFMEKRLLSFPEVETVVSKTGRAEISEDPMGPEQTDVFVMLKPRRKWGTG
                     RDTAELVEAMQEDLSEIPGLRFSFSQPIALRVNELISGVKSDLAVKAFGPDLSVLKTF
                     ADRAAAELTGIEGARDVKVEQVSGMSQLDVVVDREALARYGIKVGDVNATIETAVAGR
                     HATTLIEEERRFAVVVRFPESARTDVPELERLLVPAPGGERVPLGQLARFTVVEAPAQ
                     VSRENGMRRVVAEVNVRGRDLGGFVAEARERIAPFAKQLPPGYTVEYGGQFENQQRAM
                     RQLAVVVPVALLLILVLLYLALGSVRDSLLVLLNLPFALVGGVLAVVVFGMPVSVSAA
                     VAFIVLLGIAVQNGVVLVAYFRQLREKGETVADTVAKGCEVRFRPLVMTALTSFIGHL
                     PMLYATGSGADIQKPLAVVVMG"
BASE COUNT         5726 a        15957 c        15609 g         6229 t
ORIGIN      
        1 ctttcaacca gccgcctctc cgaggcgaag ttcaacattc aacgggacac gccccggaat
       61 agaccgagga gcattgagag ggtgacggga ttcgccgcga agggcgcggc gtgtgcgacc
      121 tcgacggctg cgataaagga cccgccgtcg agccgctcgg tcgttgccgc caggaggacg
      181 tccttccgcc gcagcggagc gatctcaaag atgcccggag gcgacttgag cgcgccctcg
      241 agcccggtct ccagagttgc gggccagtgc cccgtacggc aggctatccg caaccggaga
      301 gtgtcgttcg gcagatcgcc gataagttcc agaatgttcc tcttcaggct tcccgcacga
      361 acgaagcact catcgagcga gtcgaggtag aggaagagct tcccgccgca acccttccac
      421 gccacgtagt cagggttcct ctttagcttc gcctcaagcg cctcgtcgct cgcgacgccg
      481 ccgatatcta cgacagccga tcgctcaggc cctggttcgg atgctgcgag tgccttcaac
      541 gtccggctct tgccaatgcc gcgctcgccg agaagcacga gacacgggaa gcccatcacg
      601 tcctcaaggc gctggaggtc ggcgttggct gcacgaccga agagcccttc tgggtcggcc
      661 agaaacccgt catctgcgag cgtcaggcgc ccgtcgcgcg ggactaggaa ccgggtccaa
      721 gcgtaatcgg aagtcgtcac cttccgtgaa gtatccatca gcacgctcgt ggtcacagcg
      781 tctgtggcca ggcatttcgg gcggcgactc gggtgtcgtc agcgcacccg cattcgatat
      841 gggggtatgc cgctctgatt ccgccgtcag aaggcctggg ctagagttca gtagaactgc
      901 cgatgctcct caccctctcc accacccacc gccccgcctc cgacctgggc tatctcctcg
      961 ccaaacaccc ggccaagttc cagagcttcg agctggcgtt cggatcggcg cacgtcttct
     1021 acaccgaggc ttcggaggaa cggtgcgagg tggcgctgct cctcgaagtg gacccggtgg
     1081 gtctcgtgcg cggccggacg gcaggtgcgg aagggcttct cgaccagtac gtgaacgacc
     1141 gcccgtacgc cgcctcgtcg ttcctgagcg tcgcgattgc gcaggtcttc gggagcgcgc
     1201 tggggggccg gagcaagggg cggccggagc tggccgagac cgcgatcccg ctcgaggcgc
     1261 gcctcccggt cctcccttgc cgcggcgggg aggacttcct gcggcggctg ttcgagccgc
     1321 ttgggtacga ggtggaggcg cggcgtctcc cgctcgacga cgcgttcccg gagtggggtg
     1381 agagccccta cgtcgacgtc acgctccggc ggacggcgac gctccgggag ttcctcacgc
     1441 atctctacgt cctcgtcccc gtcctcgacg acgagaagca ctactacgtc ggcacggcgg
     1501 aggtggagaa gctcctccgg ttcggtgggg ggtggctcgc ctcgcacccg gaacgcgagg
     1561 cgatcacccg gcgctacctg aagcacagct tccgcctcac gcgtgaggcg ctcgcgcggc
     1621 tcgtttccga cgaggccgaa gacccggacg ccgacgaagg ccggcgcgac cgcgaggagg
     1681 aggccgtcga ggagcggatc agcctcaacc aggagcggat gggaacggtg ctcgccgcgc
     1741 tgaaggggct cggggcgcgg cgtgtcctcg atctcggatg cggcgacgga aagctcctcg
     1801 cccggctcgt cgacgagcgc gcgttcgagg agatcgtcgg tgtcgacgtg tcgtgccggg
     1861 ccctggaggt cgccgcgcgc cggctccacc tcgaccggct gcccgagagg cagcggagcc
     1921 ggatccgcct tctccagagc gcgctgaact accgcgacgc gcggctcgcg ggattcgacg
     1981 cggcggcggt ggtggaggtc atcgagcacc tcgagccggg tcgcctcgac gccttcgcgc
     2041 ggtcgctctt cgagttcgcg aagccgggcg cggtcgtcct gacgactccg aacgccgagt
     2101 acaacgtgag gttcgagggc ctccccgccg ggaagctccg gcatcgcgat caccgtttcg
     2161 agtggacccg ggcggagttc gaggggtggt gccgtgggaa ggcggagacc ttcggctact
     2221 cggtgcgctt cgcgccggtc ggcccggtcg accagcaggt cggggctccg actcagatgg
     2281 ccgtcttcac gagggccgag gcgtgaagat ctccatcccg aacctctcgc tcgtcgtcct
     2341 cgtcggtccc tctgggtcgg ggaagtcgtc gttcgcgcgg aagcactttc tgccgaccga
     2401 ggtcctctcc tccgacttct gccgcggcct cgtcgcggac gacgagagcg accagggagc
     2461 gacgaacgac gccttcgagg tgctccactt cgtcgcgcgc aaacggctcg ccgccggccg
     2521 gctcacggtg atcgacgcga cgaacgtcca gcccgaggct cgaaagccgc tcgtcgccct
     2581 ggcccgcgag ttccactgcc tcccggtcgc gatcgtcctg aacctgcccg agaagctctg
     2641 ccacgagcgg aacgcgtcga gggccgaccg ggcgttcggc ccgcacgtgg tccgcggcca
     2701 ggtgcggacg ctgaggcagt cgctgcgcgg gctcgggcgc gaggggttcc ggcacgtctt
     2761 cgtcctctcg acccccgaag aggtcgagga ggcgtcggtc gagagggtcc ctctctacaa
     2821 cgaccgaagg ggagagatgg gcccgttcga cgtcatcggc gacgtccacg gctgctccgc
     2881 ggagcttcgg gcgctcctgg cgcggctcgg gtacggcgag gggggccatc cggacggccg
     2941 gaaggcggtc ttcctcggcg acctcgtcga ccgcggcccc gacacgccgg gcgtccttcg
     3001 cctcgtcatg gggatggtcg cggcgggcac cgcgctctgc gtgccgggca accacgacat
     3061 gaagctgaag cggaagctcg cggggaagga cgtccgcctc acccacgggc tcgccgagtc
     3121 gatctcgcag ctcgagaagg agccgccgga gttcgtggat acggtgtcgg ccttcctcga
     3181 ctcgctcgtg agtcactatg tcctcgacgg cgggcggctc gtcgtggcgc acgccgggat
     3241 gcgcgccgac atgcaggggc gcggctcggg ccgcgtgcgc gacttcgcgc tcttcggcga
     3301 gacgaccggc gagacggacg agtacggcct gccggtgcgc ttcaactggg cgtcggagta
     3361 ccgcggggcc gcgatggtcg tctacgggca cacgcccgtt gccgagcccg agtggctgaa
     3421 ccggacgatc aacgtcgaca ccggctgcgt tttcggcggg agtctcacgg cgctccgcta
     3481 cccggagaag gagctcgtct cggtgaaggc ggagcggacc tggtacgagc ccgcgcgccc
     3541 cttccttccc gaggaggcgc aggcgcccgc tctcaccacc cagcacgcgc tggacgacat
     3601 gctcgacctc gaggacgtca ccgggaagcg gctcgtcacg acgaggctcc accggagcgt
     3661 gacgattcgc gaggagaacg cggcggcagc gctcgaggtg atgagccggt tcggagcgaa
     3721 cccgaagtgg ctcgtttacc tccctcccac gatgtcgccg gccgagacga gcgagaggga
     3781 ggggctcctc gagcatcccg cggaagcgtt cgcgtacttc cggcgcgaag gggtcccgag
     3841 cgtcgtgtgc gaggagaagc acatgggctc gagggccgtc ctcgtcgtct gccgcgacga
     3901 ggaggtcgcg cggaagcggt tcggggtcgt cggcgaggga atcggcgcgt gcgtgacccg
     3961 gaccgggcgg cgcttcttcg aggacgaggg gctcgagcag gagctcctcg cccgggtggc
     4021 cggcgcgatc gaccgggccg ggctctggga ggagctgaag agcgactgga tggtcctcga
     4081 ctgcgagctg atgccgtggt cggtgaaggc gcagcagctc ctccggagcc agtacgcgcc
     4141 ggtgggcgcg gcggcgaacg ccgcgctgcc tctcgccgcg tcactcctcg agcggtccct
     4201 cgggcgcgac gaggcgtacg gcgccgtcgc ggagcgggtg gcggcgcggg ccggcctggc
     4261 gcggcgcttc gtctcggcct accgacacta ctgctggagg gtcgaggggc tcgccggcct
     4321 gaagctcgcg ccgttccacc tcctcgcgac ggaagcgggc gtccacgttg acaggaatca
     4381 cctctggcac atggagacgc tgcggcggat tgccgaggcc gacagcgggg tcgtcatcgc
     4441 gacgccttat cgcgtcgtgg acgtgaccga tccggcgtcc gaggccgagg ggatctcgtg
     4501 gtgggaggag ctgaccgggc gcggcggcga ggggatggtc gtgaagccgc tcgacttcct
     4561 ggcaagaggg gcacgcgggc tcatccagcc ggcgctgaag tgccgcgggc cggagtacct
     4621 ccggatcatc tacggtccgg agtacacgct cccggcgaat ctcgaacggc tccgcaagcg
     4681 cggcctcggc gcgaagcgat ccctcgcgct acgggagttc gcgctcggcg tcgaggggct
     4741 cgagcggttc gtccggcgcg agcctcttcg gcgcgtccac gagtgcgcct tcgccgtcct
     4801 cgcgctcgag agcgagccgg tggacccgag gttgtgaggg tcgacgccgt cgggcgcctc
     4861 ggcccgtccc gctcccgatg gctcactcac cgaccagtcg gctcacgagc ggggggagct
     4921 ctgccagcac ctgttcgatc ggcggcaggt ccggtacgag gtcggcgagg gcctccaccc
     4981 agtgcttttc ggcttcgcgg atcaaacgct ccgtgaagaa cgaagcggta tcggtgtagg
     5041 cgacctcgcg cagggcgcac ttcgccggta gcaggcgccg gacggtcgcc gggtcgacct
     5101 cggccgcaaa cgtcgtgagg atcttccaaa ggtcgaagta gtcgcgggcc cgtggacggc
     5161 tccagccgcg taccgcgagc cgcgcatccg tctggaggag cgcgcgcagc ttctcggcca
     5221 ccacctcctc gagcgggtag gcgaggagcg atcccgcggg agcgaggccg ctccccggga
     5281 ggatccggcg cgtcgcgggt gggaggagca ccggctcgtc gtgagtgacc tcgaccttga
     5341 tcgggcagag cgcgtcgcgc tgccatggaa accggaaccg gacgacgaac gcgtcctggc
     5401 cgcccgggtg cgcttcgcgt tcccgaagcg gctccagccg cgcgtcgaac ggcccctccg
     5461 cggacagtgc cgtcacggcg cggatgcgcg ccgcgtcgag cgccgcggcg aggtccgccc
     5521 cacgcggggc ggcgagcgcg gagaagtcga ggtcctccga gaagcggtag gtatcgccga
     5581 agtagagtcg cttgagcgcc gtgccgccct tgaagacgag cgttccggag agccgggaat
     5641 cggcggcgat ggctgcgagg agcgcgccca gcgcgacgtc cttctcgagg gtcgtgagcg
     5701 gaacgcccag cgagcgcgtg agggcgagga ggcgtttctg gagcggcgtc atcggttcgg
     5761 ggtcaggttg tcctgaatgc cccaccgtcg cacgctgcgg ccgcgcgccg ggcttgccgg
     5821 atcgaggagc cggacgcccg tcgccgggag agcgcggagc ggctccagcg cctcgacggg
     5881 agcaccagcg cgctccaggg cgaagccgag gcgcttggcg accgaagtcg cgccatagcg
     5941 cagggcgtac gcgacgagcc gcccgagatc gagctcggcg aggtgctcct ccaggaggcc
     6001 aagcccctcg ccgatgccgc cgaacgcccc ggggcgcgcg aaggtctcga gagcggtccg
     6061 ctcgcggtcg gtgatcggga cgcgggatcg ctggtcgatc cacacgtcct cgatgccgaa
     6121 gtagtgggcg ggcgtgacgg tggagtacga cgctcgcagc ccgcccgcgg tctcccagcg
     6181 gtgccggccg gggcccctgc gaccggcacc gctccgcatg ctcggcgtca cgaccttccg
     6241 cgtcgtcatg caggtcacga cgcgtggcac ctgcgtcgtg agcccgtgat ggttgagggc
     6301 ggaccagtgg ctgacggccg agggctggac gatggcggtc gcgatcgcga aaggatggag
     6361 cgccgcgagc ccgggaagct cacccgtcgt cgcgtacagg ccccgccgga gcctctccac
     6421 ccagccggcg tcggcgaggc gccggagcgt cgtctcgacg gcgccaggag cgagccccac
     6481 cttggcgctg gcgttccggg cgtccgccgc tgagaacacg aatcgcttcg cctcgatgag
     6541 cgccccgatg agggcgatgc ccttcggctc ggcgtagcgc actgggtcgg gcggtcgcgc
     6601 gttcacgagg aactcccacg caaacgtaca ctatgtgcgg ttacgtagca aaacagtgag
     6661 aacgatcggg gcgcccgatc tcgaacctct gacgacgctc ttccgcgcgc tgggggagga
     6721 ggcctgggcc gcgcctaccc gctgaaacga acgacgcagt tccccttctc gtccgcctcg
     6781 acgtcgaacc ggaccggcag gaacgcccgg atgacctcga tgttcgtcgt ggcgtggcgc
     6841 gagagaggcg tcgtgcggaa ggcgcccgag ccggcgaggg ccatcgggag gaggagctgg
     6901 tcggcgaggt gctcgccgac cggaacgccg gaggcgaggt agtccctcgc ctcgcgcgcg
     6961 accttctcgg cgaccgcctc ggcgctgatc cccttctcgc cgaaaccggt gaagacctcg
     7021 gtgaggcccg cgctctcgat ctcgagcgtg acgacgttgc cggggccgga ggagtcgacg
     7081 gaaacggcct cgagcctctc ggcctcccac ccgaggagac gcgagacgac ggcgagctcg
     7141 cgcgtcgcga tctcgcgcga gagcttcgac acgacggccc gcgcggcgcg ccgcttcacc
     7201 tcgccgcgct cgaggagcac gagcggccgg agtcgcgccg cgggagtgac cgtgacgatg
     7261 cactcgcctc cgccggccgg gtagaagccg ggctgcacga ggacggcgtc gaccgtcgca
     7321 cccatgcgcc cgaccagcgg gagaaaggcc ttctccagga agtcgaaggg cggggccatc
     7381 gggttgtgcg tcccgccccg gaggcggacg gtgctcgggc cgcccgccag gacgagcgcg
     7441 gggaggatcg tctggaggac gagcgtcgtc gacccggccg tcccgaccga gaagtcgaag
     7501 ctcccgtgcc ggagcgcgcc cggctcgaac ctcagctccc gcgaaccgat ctcggcgccc
     7561 ttcacgtgcg ccgatccgac ggccgcggcc gcctggacgg ccgtcaggtg ctgccgcatg
     7621 aggccgggct tctgccggcc cgcgcggatc cgcgtgatct ccaccgggcg gcgcgtcgtg
     7681 agggagaggg cgagggcggt ccggaggacc tgaccgcccc cttcccccat ggagccgtcg
     7741 atccggacga ggcccgtcat cccttcacgc agacgacctg gcggagcgtg tggagcacct
     7801 ccacgaggtc cctctgcgcc tccatgacgg cgtcgatcgg cttgtaggcg gcgggcgtct
     7861 cgtcgatcac gtcctcgtcc ttccggcact cgatcccttc cgttgccttc gcgtggtcgg
     7921 ccacggtgaa gcgccgccgc gcctcggcgc gcgacatcga gcgtccggcc ccgtggctgc
     7981 aggagtggaa gctctccggg ttgccgagac cgctcacgat gtatgagcgc gttcccatgc
     8041 tgccggggat cactccgagg tctcccttcc gtgccctcac ggcccccttc cgcgtgacga
     8101 agacgttctt cccgtaatgg tgctcgcgct cgacgtagtt gtggtggcag ttcacggcga
     8161 cgagaccggc cgtgaagggc gggaggcccg gaagcgcgcc gagcgcggcg acgacgttcg
     8221 ccatcatgag cctccggttc gtcatggcgt actcctgcgc ccagccgacg gcggcgacgt
     8281 agtcgtcgaa gtgcttcgtc ccttccggca ggtaggcgag atcggcgtcg ggcaggttga
     8341 tgaagtgccg gcgcatgtcc tccttggcga gctcgatgaa gtagctgccg atcctgttcc
     8401 cgacgccgcg cgagccgctg tggagcatga gccagacgca ctcgtcctcg tcgaggcaga
     8461 cctcgatgaa gtggttccct gtcccgaggg tcgcgaggtg gttcacgtgg ttcgccttcc
     8521 ggatcgccgg gtgcttctcg acgagcgcct cgagccgcgg ggcgagaggc gcccacgcct
     8581 cggcgacggc gtcgggcggc tcggcccagg cgccgcggtc gttccgtccg ccgttgtcgg
     8641 tccggccgtg gggaacggcc cgctcgatcg ccgtccgtac ggggtggagg tcgtccggga
     8701 ggtcgcgggc ggcgaggtcg gtcttgagcg ccatcatccc gcagccgatg tcgaccccga
     8761 cggcggccgg gatgatcgcc cccacggtcg ggacgacgct ccccaccgtc gcgccgatcc
     8821 cgaggtggac gtcgggcatc gccgcgaccc acttgtggat gaacgggagg cgggcgatgt
     8881 tcgcgagctg cttcttcgcc tcgggctcga acgggacgcc ccgcgtccag ctcttcaccg
     8941 ggacgccgcc cgggatgtcg atcacgtcga agttgcggtc ggtcatggct tctcccgaag
     9001 ggcgcgacga ggggtgtcga aacggactgc tctacccggc tgagctacgg cgctcgtctc
     9061 ccgacggcac cgacgggatt cgaacccgcg acatgtagtt ccgaccgcat tcgcgcctgt
     9121 cgatttcgtt cctcgattgc ggcgacgagg agtcgggaga cgctcccggc gctctgtcct
     9181 gagctctcgc ttgcgcgatc cgggcatcgc acccggcctc cggagtgact gtagtccccc
     9241 tggcattcgc cgcgttcact tcaatgagcc gatgatgggg cgacgaggtt cggcgagagc
     9301 tgcggggttc gagcccgctg aacgaccatg tactcccgcc ggcattcgcc cgtcgtcgtc
     9361 gttcgatcga gatgccgggc cgacgagggg acgccgagac gttcacatgg tgtagatgta
     9421 gtcccggcgg cattcggccc gacgatctca ggttacttca gagtacgacc tcctcgaccc
     9481 gacccaccca gtggtcggcg gccagcttcc cctgcgcgaa cgcggagagg acgttgaaga
     9541 cctcgtccga gaagccgccg acgttcagga cgtcgtcgcc ctcggccgcc tgcgtcgtgc
     9601 cggacggctg gaggtcgacg caggcgagct tcgctcccgg gttccgcttc cggaaccgcg
     9661 accactcgcc catcagcgcc gacccccggc ccgtcctcgg gtcggcccac gactcgttgt
     9721 cggagacgaa gaccacgagg tctcccttcg ccctccgccc gttcagcgct tcgagcgccg
     9781 ccgagcagtt cgttccacct cctccgatcg cggcgagctt cctcgcgttc gtcacgaccg
     9841 agtccctcgg gttcaggttc accggcacca ccttctgctc gaacgggagc acctcggcct
     9901 tcgggttggc ccgcacgatc gccgccgaga cgagcgccgc gacgtcgatg caccggaccg
     9961 cggttgtcgc ccccttccgg tggccggtca cgggcgagga catcgagccg gagacgtcgg
    10021 ggcagacgaa gaccttccct tcgatccgcg gcaccttcgc gatcgcgacc tccatcgcgt
    10081 cctgaagcgc ttcccggacg accggcggca cgccctccct cgccgaggaa tgcgcggcga
    10141 ggagctggta tgggaagacc ctcgacctcg cgatctccct cgggttcgcg agccgcgacg
    10201 cgacgaggtt ggcgaggccc ggcacctcga agacgccgtg ccgcgcgaac gtgttcaggt
    10261 tcatcctcgt catctgccac gacgccttcc gcgcgatggc gacccactcg tcccggccga
    10321 gctcgagagc cgtgagcatc tcgaacggga cgttcgggac ctgccgcgtc ccgctcgctc
    10381 ccgccgactt gaacgcttcg aactgccgga cgatctccgg gagcgcggtc tcgtcgacct
    10441 ccttcccgat gaggtagccg tagagcgcct gccgcgcccc gtcggccggc ttcggatgga
    10501 ccatcttgat gacgtcggcg agcgacggct ggttcccgac cgacgcccgg aagagcgccg
    10561 tgccgctctt cccggcaagc cactccctca cgagccgctt cgggaccgtc ccgagcgact
    10621 tcctccccac ggcccccgac cggacgacct ggacgaagtt ccggagcatc ttcccgttgt
    10681 cgacgacccg cgggaacgcc ttcgcgaggt gttccggcga ccgcgtcgag agaacggcgc
    10741 agaggagggc cggggcgtcc ttcatccagg cgctctcccg ggcgaagacg gcgagcttcg
    10801 cgaggaactc ggagtcgacc ttcgcggcga gggcgaggag cttctcgagc tggtcgtcgg
    10861 ccgaggcgta gaacgtcgcg ttcaggcagc ccgtcgccac gtactgggcg agggcctgct
    10921 tcggcgtcct ctcgtacgcc acacctcccg cttcgttcgt ggcgttggtc gccgggacga
    10981 gctttccgac gagcgacctg aagagcctcc tgttcgccat ggtttcctcc gcgtcccttc
    11041 gtttcctgtg gaccgagatt gcgaaggcgg tgccatgccg ggcggtagag gcccggccgc
    11101 cgaacaagca tctgaaaggc aaggacttgt cggggaaggt cgcccttcgg agcgttccca
    11161 ggtggtttcc ggatcgatct tctaggataa gcagcgatat cctagcggat catggcacgc
    11221 cgcaagcagg tcgtcttcgg gctcctcggc gccacgctgg accgcggcga gggctccgag
    11281 cggtgggaga cgtggcgccc gacggtctcg ctcttccagc atgaggagct gctcctcgac
    11341 cggctcgcgc tgattcacca ggcccgtttc gcgaagctcg cccggcgcct cgtggacgac
    11401 atcgcgtcca tctcgccgga gaccgagacc cgcctccacg ccgtggagtt gcgggacccg
    11461 tgggacttcg aagaggtcta cggcgcgctc cacgacttcg cccgcgcgta tcccttcgac
    11521 cccgagaggg aggactactt cgtccacatc acgacgggca cgcacgtcgt tcagatctgt
    11581 ctcttcctcc tcacggaggc gcggctcttc cctgcgcggc tcatccagac gtcgcccccg
    11641 gaacgccgcc actcgtcgag ccccggcacc taccaggtca tcgacctcga cctctcccgc
    11701 tacgaccgga tcgcggcgcg attccggaag gagcggcggg aagggctctc gttcctgaag
    11761 tcgggcatcg cgacgaagag cgagcggttc aacgggctca tcgagcggat cgagagggtc
    11821 gcggtcgcct cgcccgagcc gctcctcctg atggggccga ccggggccgg caagtcgctc
    11881 ctcgcccggc ggatctacga gctgaagaag gcgcggcggc agatctccgg tcccttcgtc
    11941 gacgtgaact gcgcgaccgt gcgcggcgac ggcgcgatgt cggcgctctt cggccacgag
    12001 aaaggggcgt tcacgggcgc cgcggagagg cgctccggcc tcctgcgcgc ggccgacgga
    12061 ggcgtcctct tcctcgacga gatcggcgag ctggggcccg acgagcaggc gatgctgctc
    12121 cgggcgctcg aggagaagag gttcctgccg ctcggctccg accgcgaggt gaggagcgag
    12181 ttccagctcc tcgccgggac gaaccgcgac ctccaggagg acgtcgcggc gggccgcttc
    12241 cgcgaggacc tcttcgcccg gatcaacctc tggacgttcc gcctgccgcc cctccgcgaa
    12301 cgtcgcgagg acatcccccc gaacctcgac tacgaactcg accgcttcgc ggagcggagc
    12361 ggagcgaccg ttcggttcaa tcgcgaagcc cgggagcgct tcctcgcgtt cgcgatgtcc
    12421 ggggaagcga cctgggacgc gaacttccgc gacctgagcg ccgccgtgac gcggatggcg
    12481 acgctggcct cgggagggcg catcaccgtg aacgacgtcg accccgagat cgagaggctg
    12541 aaggcacagt ggcgccggcc gggcgcgcgc gtcgcgccgg actctgccga cggtctcgtc
    12601 ggcatcctcg ggcccggagc ctccgggatc gaccggttcg acctcgcgca gctccgggag
    12661 gtgatccgcg tgagccggga gtcccgttcg ctctccgagg cggggcgcac gctcttcgcg
    12721 gcgtcgcgcg cccggaagag caacccgaac gacgccgatc gtctccggaa gtacctcgcc
    12781 cggttcggtc tgacgtgggc gcagatctcg ggtgcgtaga tccgcgttcg atcccgacgg
    12841 ccggcccaag acgggccgcc ctggtggtct ggggcgggac ggcgtccaca gaacggccgc
    12901 gccgcaaccc cgcctctccc gctcgcgatc tgtgacactc cgcgcatgaa gacgatcgga
    12961 ctcctcggcg ggatgagctg ggaatcgacg gtgccgtact acgtcacgct gaaccgcgtg
    13021 gtggcggagc ggctcggggg cctgcactcc gcgagggtcc tcctgtcgag cgtcgacttc
    13081 gcgccgctcg aggccctgat gcgcgcgagg gactgggacg agatcgggaa gacgctcgct
    13141 cgggaggccg tccgcctcga agaggccggg gcgagggtgc tcgtcctctg taccaacacg
    13201 atgcacaagg tcgcggagac gctggaggcg gcggtgggga tcccgctcgt ccacatcacg
    13261 gacgtgacgg gaacggccgt tcgcgccgcc ggcctcaggc gggtggggct gctcggaacg
    13321 cggttcacga tggaggagga cttctacgcc gcgcggctcg cccgcctcgg gatcgaggtg
    13381 ctcacgccgc cggccgatgc acgcgccttc gtcgaccgcg tcatcttcga ggagctctgc
    13441 cgcgggcagt tcctcgcgtc gtcccggagc ggctacgccg aggtgatgca ggacctcgtc
    13501 tcccgcggtg ccgaggggat cgtcctcgcc tgcacggaga tcgggctgct gctcggcgac
    13561 gcgccgttcc cggtgccgct cttcgacacc gccgagatcc acgcgcgggc ggccgcggac
    13621 gccgcgctcg cctgagggga cgaaccggcc cggctccccg gcgcgagggt cggcgacacc
    13681 ggccgatttc agcgccccgg cgaaattttc tcgtcggctc gtagaattcc gccctcgccg
    13741 ttcgtcgttg gtgtgaaggt cgggcagaaa gcccgcccga aggacaggag tacgccatgc
    13801 cgcagtacct ggttgccggt tacctccccg acgacttcga cccgtcgcag atggacgagg
    13861 cggtgggtcg cgagatccac gcgctcaacg aggagatgat cgccgccggc gtccggaagt
    13921 tcgcctgcgg tctcggggcg gcgaagtctc tgcggcccca gtccgacggc ggggtcctcg
    13981 tcaccgacgg gccgtacctc gaaaccaagg agcacatcgg cgggttctgg atcctcgaat
    14041 gcgcggacat ggacgaggcg gtggcgtggg cgcgcaaggg ggtcgcggcc acccgggggc
    14101 aggtcgagat ccgggagatc ttcttcgttc cgactcccga atagcccgcc gcgcgccccg
    14161 cccgacgtca ccgacgccgc ccgacctcac gggggggatc cgagagagct gtcgcccgag
    14221 ctcgctggag aagagatgcc gcagtacctg atcgccatcc aacaccccga ggactacgac
    14281 ccgtcgctgg agggcgaggc gatgatccgc gacatcgccg ccctcaacga agagatggac
    14341 gccgccggag ctcgcttctt cgccggcggt ctcgaatcgg cgcgccacgc ccgatcgctg
    14401 cgcaaacagc ccggcggcgg ggtctccatc accgacggcc cctacctcga agcgaaggag
    14461 cacgtcggcg gcttctggat cctcgattgc gccgacctgg acgaggccct ggcctgggga
    14521 cgcaaggccg tcgtcgcctg ccgtgttccc gtcgaggtgc gagccttcct gaggatccgg
    14581 ccgaagcggg aatcgcccgg ccgcggaggc tcggcgtgag gaggctgacc cggtagcctc
    14641 acgagcatga gcggtgtcca cgagacgatc gaggccgtct ggaggatcga ggccacacgg
    14701 ctcatcgccg cgatcgctcg cgtcgtccgc gacgtcggcc tcgccgagga gctggcgcag
    14761 gaagcgctgg tcaccgccct cgaggtctgg cccgaggagg gcatcccgga tcggcccggg
    14821 gcctggctga tgacggcggc gaagcggcgc gcgatcgacg ccctgcggcg cggccgcatg
    14881 ctcgaacgga agcatgtcga gctcggcctc gagctggagc ggcagcagga gcggctcgga
    14941 gacgccctgg atcacgcgct ggacgaggtg atcgacgacg acgtgctgcg gctcgtcttc
    15001 accgcgtgcc atcccgtgct cccggtggag gggcgcatcg cgctcacgct gcgcctgatc
    15061 ggcggcctca ccacggccga gatcgcgcgc gcgttcctcg tccccgagaa gacgctgggc
    15121 cagcggatct cccgcgccaa gaagaccctc gcggaggcgc acgtcccctt cgagacgccc
    15181 cgcggggacg agctgaaccg ccgggtcgag tcggtcctca cggtggtcta cataatcttc
    15241 aacgagggct acaccgcgac ggcgggcgag gagtggatgc ggacggccct ctccgaggag
    15301 gcgctgcggc tggggcggat cttgcggaga ctcctcccgg gcgagtcgga ggtccatgcg
    15361 ctcatcgcgc tgatggagct tcaggcctcg cgcagcgcgg ctcggcgcgg cccggacggc
    15421 gaggcggttc tcctccctga ccaggaccga tccctgtggg accgcgctca gatccagcgg
    15481 gggttcgccg ccctcgagag ggcgcagagg ctcggcggcg gcgcgcggcc ctacgcgctg
    15541 caggcctcca tcgcggcgtg ccacatgcgc gcccgcacgg cggaggagac cgactggggc
    15601 cgcatcgtcc tcctctacga cgccctgctg caggtccttc cttctcccgt cgtggcgctc
    15661 aaccgcgcgg tcgccgtcgg catggcctgg ggcccggccg cggggctcga cgagctcgac
    15721 gcggcgaccg cgctcgccgg cgagtccgcg accgccagct accacctctt ccccagcgtt
    15781 cgcggggact tcctgaggaa gctgggccgg ctctccgagg cgcgagacga gatccggcgc
    15841 gccctcgcga tgacgaagaa cctgcgggag agggagctcc tggagaagcg gctgaggcag
    15901 tgggagaagg cgaccccatc cgcgtagcct cgctgcccgc gcgctccctc ccgctctctc
    15961 tcctggccca gctcttcgcg tcgatgaagt cggggtgcgg gacgtggagg aggccggcga
    16021 cacggcgaat gaggtgctcc tcgcggaggt gcttctcggc gtcggcgaag gcgacgcgcc
    16081 acatcagctc gacgatccgc ttcttctcct cgagcgcgaa cccctcgtcg acgaggctcg
    16141 tgaactcgta gagcgaggtc gcgacgttcg cctcgacgcc ggggaaagag cgtccgaggg
    16201 cacctggagc gcggcgcgga cgaccgtccg ctcctcgtcc tcgacctcgg ggtcggctcg
    16261 catcaccccg acgaggagca cctcggccga aagacggtcg cgccgactcg gatcgcccgc
    16321 ggcttccgcg ggggcttcgg tgaccgctcc tggcagaacc gctggtggcc gtcgaccagg
    16381 gtgtaatcgt cactccgccg gcggccttcg cccggacgat ggaaacaggg ctcgtccgtt
    16441 ccgggacgcg cgacggtgag gtaccttcgc cgaggcgcac tcccagggta gaggggggaa
    16501 ggtatgccca tacgttcctg ctccgccgcg ctctccggac tcctcgcgct cgcaatgctc
    16561 ttcgtcgcgg ggccgggacg cgccgacgtc gtctggaggg tcgccgacga ggggccgcgc
    16621 gcgcccgaag ccgtccagat tctcggggac gagacgcgga cgctcgcgtt cggcccctcg
    16681 ggggactggg agctgcgggg aacgacgtgg cacgccgtcc cgctccgcac gacggaggtg
    16741 gtcggaagcg agcggacgct cttcttcgcg cgcgggcggt tcggggcgac gaccaggagc
    16801 agctccagtt gccgggttca gctcttcacc ctgaacggcg acacgtggac ccggctctgg
    16861 agcgagacgt cctgccatcc ggtcgtacgg tcggacgaaa ggctctacgt cgtggcggag
    16921 gccttcagga cctgcggctg tgcgagcgat ccttccctcg ggccggaggc ccggcgcctt
    16981 cgctccgtct ccctcgcgga cggttcggtc cgggaggagg cgcccctccc cgcctgttcc
    17041 ggcacgctct tcgcgctggc ggggaagctc cacctgatcg ggacgccgcc gatctgcgga
    17101 ggtccgagct ccgacgacgt cgccgccctc gtgagcacgc cgccctatga cttctaccgg
    17161 ctcgacgagg ccgggtggac gcagctgcct ccgtgggacc tgcccgtctg gaccttcttc
    17221 gaatcgacgc cgaacagcct ctgggtggtc cacgcgctcg gcggcgtcga ctacgtcgcc
    17281 cgaatcctca cgaccaccgg cttctcggac ccggtcccgg ttccgcgcga catcacggtg
    17341 agctcgggag gtccgaaacc gctcgaatgg aacgggcgcc tcctcgccac gagccgcgag
    17401 ggacggggca acatctacct cctcgagggg aacgccctcg tccggatgac gccggtctcg
    17461 ccggtgaccg cgcgatatcc cgcgccgctc gtctccgtcg ccgggaaccg catcttcacg
    17521 tcggccgacg gctgggacgt gcacgaacgg gatgggtcgg agtggatcgc gacgaccggt
    17581 gtggtcgggg ctgccggggc cgattcgtat ctctcgggcg aaacgaagac gttcgccgtc
    17641 cgcggttcgc ggctctaccg ccgggaggac gccggctgga agcgcctccc gccctcgccc
    17701 ggcgccgagt gggcggaggt catcggtgtc tggaaggacc ggccggtcgt cgccgagtgg
    17761 gtctcgaatt cgacctctcg ccggcatctc gcgctcgacg acgagtccga ggagtgggta
    17821 gacctgaaga gcccggaggg attcgacgcg ccgctgctcg gtgtggggca ggacctctat
    17881 ctctccggcc ccgccgggag gctggcacgg cttcgtaacg gggcctgggc gattctcgag
    17941 gcgtcgccgc tcccgtccga gcagtgcacg agcaggcccg tcgcgaaccg gcgggtgggc
    18001 ggcgagacgt atgccgtgtc gaccgactcg tgcttcggtc ccgaccgacg cgacgaggcc
    18061 gtcttccgcg tcgacggcga ccggctcgtc cgtgccttcc cgaacctcga tcgcgcgatg
    18121 cgcgtcttgg acgtgctcga ggtcgacgga gagcccgtcc tgcaggtcca cgacgcatcc
    18181 ccgggagtcg ggccgcgggc cgttctggtc aggccgacgg aggccggttt cgatacgctc
    18241 ctgacgcgcg acgacgttca ggagagcggg cgcgactcgc ttgcgcgggc cgagatgtcc
    18301 agcgccggaa gctcggtctt cttctcgggt ctccgcttcg cccggggccg gctctccagg
    18361 caggggggtg gaaccgtccc cgcgaagatc gaccccgatg gccgcatcgc gtacggagac
    18421 cggtatcgat tcgcctccga cgctcggccg gacccgctcc tcacgctcgt ccctcgcgtc
    18481 cggaaacaga tcgcggccgt cgtggatgcg accggacacg gcgggacccg ctaccgaagc
    18541 gagctgacgg tggcgaacct ctcgccgggg gccgcctgcg tggcgaaggt ctactccggc
    18601 gcggagaccg agccatcgct cgaggtcccg ctcggacccg gtgcgcagaa gcgggtcgcg
    18661 gaccccgttc ccggcttcgt cggtcctctc gccgtcgagt tcgaagggct ctccgacgac
    18721 cgggaggcgt gggcggccgt gcgcgtcttc agcgcgtccg gaggcgggac ggcagggacg
    18781 tcgattctcg gaagcgaggt gggaagcctc gccgggacga ccacgctgct gcgcccgatc
    18841 cgcgcgccgg ggagccgcct gcacctcgcg ctggcgacct cccgggacgg aagcgacgtc
    18901 ccgacgtggg cctacgcgga cccccgtcag gccccggaga tccacgcgag cagtccgctc
    18961 ccggccggtt cattcctcca ggtcgacccg gacccttcga agctcttcgg gccgctccga
    19021 atcgaggcgt ccactctctg gctctccccg atcgacgacg cgcgcaacga cctcctcggg
    19081 tacttcgtga ggaacgacgg cgcgacgaac gacgggacgg tcgtgccgtt cgaggagccg
    19141 gacgccgttc ccggccggcg cacgcggttc ctgccggccg tcgtcggcgt gacgtcggag
    19201 cacgggcggt accggaccga gctgacgctc ggccggaggg agcgatgggg cctcccggag
    19261 tcagggctca ccttcaccgt cacctaccgc gacgagaagg gcgcgtcgac gttcccggtc
    19321 gccgtcgacc tcgacggcct cgtggaggtg ccggatgccg gcgagtggct cgtcgcaaac
    19381 ggcgtcccga tcgacccgaa cgatttcgcg gggacgctca cgttctcgtc ggatcggccg
    19441 gaaggcgcgg ccgacctcct cgtgacggcg gtcgtccagg cgcgggccgc cggcgcggcg
    19501 ggcgactacg gcgtcagcgt ccccgtcgtg aacgaagtcc gatgggccga tgggcgcgcg
    19561 gtcgtcccgg ccctgcgcga ggatgccggg ttccgctcga acgtcgccgt cgcgaacccg
    19621 gagcccgagg gcggaccggt cgtgactctg gaggtgacgc tccgccaggc ctccgatggg
    19681 tcggtcatcg ggaccctccc gcgcgtgacg ctcccgcccg gccggcggtt ccagtggaac
    19741 cggccactct cgcagatcgg atacggcggc gacgcctggg ccgaggtgcg gcggctcgga
    19801 ggctcgggcc ggttcgtcgc gtacggagtg gtgaacgaca acgtgacctc ggacgggacg
    19861 ctgctgccga tgacgcagcg gcggtagaga ggcttcgcgg gcggggccgc gggttcgcgc
    19921 caccgccgcg tctcgcgccg cgcccgggcc gcggatgccg gcgagcgcct aagatcccgt
    19981 acgaatgccc ctctatcgcg caggccgtga cctcgtccgg ctcgaggcgg cggagcccgc
    20041 cgtccggggc cctgccgcgt ccctctgggc cgaggtgccc gacaccgacg cccgcgccga
    20101 cgagctccgc ctccgggtcg aggtgagcgg cgcggcggtc gagggcgacc cggaggaagc
    20161 gctcgtttgg gaggtgagca acgacgagac gcgcctcgtc gcgcctggga tcgagatgac
    20221 gatccggccg cgggagcgtc gcgcctcggc cacggtggcg cgttcgctcg tcgggcttcg
    20281 ccccgggctc gtggcaaggc tcctcctgga ggcgcccgtg acggcgatga agatggtgac
    20341 gatgcagctc ctccacgcgg gcgccgtcgt gggcccggcg ggcgccgccg tcgtgcgcgg
    20401 agcggcggga gcgggaaagt cgacgctcgt ggccgcctgc cgcgcggccg gcctcgggtt
    20461 cctcggcgac gagagcctcc tcgtcgaccg ggacgacgcc gaccgccacg aggcgacgct
    20521 ccgcgacctc gtcctcacgc cggagtccgc ccgcctgctc ggcctggacg cggcgtcggt
    20581 cccggccttc tcgggcggtg agcggaagcg gcgcgtgccg ctgccggagc cgctcgcgcc
    20641 ggccgaccgc ctcgcacgcc gcgtggcgac ggtcctcctc gggccgcgcg agccgggtcc
    20701 cgcgcggctc gtcccgatct cgggagccgc gttcctcgag tcgttcgcag ccggcgaaat
    20761 tccgcaggag cggatgtacg ggggagatcc cgcggcgatc gccagggcct gggtcggccg
    20821 ggagacgtac ctcctgacgg gagccgccga cctcggaggg gccgtcgacc agatccgtga
    20881 tctggtgcac cgattcggcg cccgctgaat tcctagactg cgtctcaaag aatgagcgca
    20941 gccgggcccc ccgtcctctt cgcccactcc tgcttcctcg cgcacgaccc gaagcaggtg
    21001 aggaagatgc gcccctaccc gccgctcgcg acgctcctcg ccgcctcggt cgtccggcgc
    21061 gcggggcggg aggtcgccct cttcgacgcg atgctctcgg cgggcgagga ggagttcgag
    21121 acggccctcg cgcgcgtcgc cccgcgggtc gtcgcgatcc tcgaggacaa cttcaacttc
    21181 ctgacgaaga tgtgcaccca gcggagccgc gaggcgtcgg tccggctgat ccggagcgcg
    21241 aaggcggccg gggcgcgcgt cgccgtaaac ggctccgacg cgtcggacga cccggcgttc
    21301 tacctcgacg tcggcgccga cgccgtgatc ctcggcgaga cggagcggac gatcgtcgac
    21361 ctcctcgacg cgtgggaccg ctcccccgac gccgacctgt cccagatccc gggcctcgcg
    21421 ctcccggcgg gcgccgtccg cgcggccggc agcgccccga cccggacccc gccgcgcccc
    21481 ttcgtcgcgg acctcgacgc ccttcccctc cccgcgtggg acctcgtcga cgtggagcgc
    21541 taccggaagg cgtggacgga ggcgcacggg cgcctctcgt ggaacatggt gacgacccga
    21601 gggtgcccct accgctgcaa ctggtgcgcg aagccgctct acggcgtccg ctacgcgcag
    21661 cggagcccgg agctggtcgc cgcggagatg aagctcctga aggagacggt cgctcccgac
    21721 cacgtctggt tcgcggacga catcttcggc ctctcccacc gctggacgga gcggttcgcg
    21781 gaggaggtcc gggagcgaga cgcctccatc ccgttcacga tccagtcgcg cgtcgacctg
    21841 atgtcgccga ccgcggtcga ggcgcttcgc cgcgccgggg ccgaggaggt ctggatgggg
    21901 gtcgagtcgg gggcgcagtc gatcctcgac gcgatggaca aggggacgac gatcgcccag
    21961 gttcgcgggg cgacccgccg gctgaaggag gccggcatcc gggcttcctg gttcctccag
    22021 ctcggctacc ccggcgagac atgggccgag atcctcgcga cgcgcgagct cgtccgcgag
    22081 gagcgtccct ccgacgtcgg cgtctcggtc gcctatcccc tccccgggac ggtcttctac
    22141 gagcgcgtga agagccagct cggggagcgg tcgcactgga aggacagcga cgacctcgac
    22201 atgatgttcg aggggaccta ctcggccccg ttctacaagc gggtccgcga cctcctccac
    22261 gcggaggccg ccctcggggc gatcccgccg tcggagacga tcggccggcg tcgccgcaac
    22321 ctcgacgcgg cgtggctcgc cgccgagcgc gacgagccgc tctctcgcac agcgggcgcg
    22381 gcgctcccga cccggcagcg gggctgacgg gcagcctccg gggcgagccg atggccgacc
    22441 tgctcctttc gcacggctac ttcctcgcgg aggacgagaa ggagcgggcg gtcatgaagc
    22501 cctacccgcc gctcggcctc ctctcgctct cggcctacct gaagcgggag gggttctcgg
    22561 tcgaggtctt cgacacgacg ttcgcgacgc gcgaggcgct cttcgcgcgc ctcgacgaag
    22621 ggcccggcgt cctcggcctc tacacgaacc tgatgacgcg cccgcccgtc ctcgcgatca
    22681 cggccgcggc gaagacacgc ggctggacgg tcgtcctggg cggccccgag agcgcgaact
    22741 acccggccga gtacctcgcc gccggcgccg acgtcgtcgt ccggggcgag ggagaggaga
    22801 ctctccgcga gctccttccg gcgctcgccg cgcgaggcct tcatcggctc gatgggattg
    22861 ccggcatcgc gttccgcgac gagacgggcg tcgtcgtctc cggcccgccg cgcgctcagg
    22921 tccgctcgct cgacgcgctc ccctggccgg accgcgaggc gatcgaccac gagccgtacc
    22981 tcgcggcctg gcgggagcgg cacggcgaag ggagcgtcaa cctcatcacg gcgcgcggct
    23041 gcgcctacaa gtgcgactgg tgctcgcacg ccgtcttcgg caacacccac cggcggcgct
    23101 ccccggcgaa cgtcgccgcc gaggtggagt ggatccgcga ccgctaccgc cccgagcagg
    23161 tctggtacgc cgacgacgtc ttcacgatct cgcaccgctg gctcttcgag tacgccgcgg
    23221 agctcgagaa gcgcggcctg aagttcccgt tcgagacgat ctcgcgggcc gaccggatgg
    23281 ccgacgagga ggtcctcgcc acgctcgcgc gcctcggctg ccggaggatc tggatcggct
    23341 cggagagcgg gagccagcgc ctcctcgacg cgatgcgccg cggcgtgacg gtcgagcagg
    23401 tgcgctgggc gacgcacgcg gcgcggcggc acgggatcga ggtcgggatg ttcctgatgt
    23461 ggggctacga cggcgagacc gccgaggacg tcgcggcgac ggtggagcac gtccgcgcct
    23521 cgctccccga cgtctacttc acgacgctcg cctacccgat catggggacc ggctactacg
    23581 agaaggtgaa ggggcgggtc tccctctcgc ggccgtgggc cgaagggggc gaccgcgacc
    23641 acgtcgtcgc gggacgcgcg ggccgcgaca cctaccggct cgccgaccgc tggctccgcg
    23701 cggccgtcga gtcgcggcgg ctcgaggaga cggcgccgga agaggcccgc gccctcgccg
    23761 gcgaggcgga cgcggcccgg cgcgagttcc tggccgcctt cgcggagggg gacgcgtgat
    23821 cgcggcggcg agagggttcg acgccctcgc cgcgacgtac gacgaggagt tcacccgaac
    23881 cgacctcggc cggcgcctcc ggtccgccgt ctggcggcac ctcgacgcgg cgttcgcgcc
    23941 gggagcgcgc gtcctcgagc tcggctgcgg gacgggagag gacgcgatcc acctcgccga
    24001 gcgcggcgcg cgggtcgtcg cgaccgacgc ctcgggggag atggtcgccg agaccgtccg
    24061 gaaggcgggg gcggcgagcc tctcgcacct cgtcgaggcg cgccgcgtcc cgttcgagga
    24121 gctcgacgcg gccgggccgt cgctcggcac gttcgacggc gccttctcga acttcggcgc
    24181 cctcaactgc gcggcctccc ttccgcccgt cgccgaggcg ctcgggcgcc tcctcccgtc
    24241 cggcgcgccg ctcctcctcg tcgtcatggg ccgatgggcc ccctgggagt gggccttcta
    24301 cctccgccgt ggcgacggag cccgcgcgtt ccgccgcttc cgcagaggag ggacctcctg
    24361 gaggggcctc accgtccgct acccctctgc cggccggctc ctccggacgt tcgccccctg
    24421 gttccggcgg aggaggctgg cggcgctcgg tgtcttcctg ccgccgagct atgccgcctc
    24481 ctccgtctct ccttcgaccc tcgcgctcct cgaccgctgg gagaggcgga tcgaatcggc
    24541 gcctcttctc ccgcgcctcg ccgaccacac gctcttcgcg ctggagcggc gatgaccgag
    24601 ctcgccgcgg cggcgcggcc ggtgccgtcc cgcgcggagg gggcgctcct ccccgccgtc
    24661 gacccggtcg tctcgctccc gatcgtcacg ctcctgccgt tctcccgctg caactgccgc
    24721 tgcgccatgt gcgacatctg gaaggagagg aacggcgccg cgctctcgcg ccacgatgtc
    24781 gagcggctcc tcccggacct cgctcggctc gggacgcgac gcgtcgtcct ctcggggggc
    24841 gagccgctga tgcaccccga ccttttcggc ctcgtcgcgc cgctccggga ggcggggatc
    24901 ggcctgacgc tcctctcctc cggcctcatc ctggagcggc acgccgccgc gatcgccgcg
    24961 acgttcgacg acgtcgtcgt gagcctcgac ggcccggccg aggtccacga cgcgatccgc
    25021 ggggtgccgg gcgcgttcga gcggactcgg cgcggcctcc gcgcgcttcg cggcggcgct
    25081 cccgttcccg ccctctcggg gcgctgcacg gtccagcggg agaacctcgc gcaccttcgc
    25141 gcgaccgtcg cggccgcgcg cgacctcggc ctcgacaggg tcagcttcct cgcggccgac
    25201 accacgagcc cggcgttcgg gcgggcggcg gggtgggacg aggagggccg ccgtcgcgtc
    25261 gcccccgacg gcgcggacct cgcgcgcctc gccgccgagc tggaccggct cgagctggag
    25321 aacgccgccg acctcgcctc gggcttcgtc tccgagagcg cgtcgaagct ccgcgagcgt
    25381 ctcctcgcgc acttccgcgc ggtcggcggc gaggcgtcct tcccccgctc ctcgtgcaac
    25441 gcgccgtggg tctcggccgt cgtcgagacc gacctctccg tccgcccctg cttcttccat
    25501 cccgcctacg gccggatcgg cgagggcggc ctcgccgcgg tcctgaacgg cgaggcggct
    25561 ctcctcttcc gccgctcgct cgacgcggcc gccgaccccg tctgcctccg ctgcccctgt
    25621 cgcctgaacc tgcgcgacgg cgcgggaggg acgccgtgag ccgccgccgc gtcgtcctct
    25681 acaacccgcg ggcggtcttc ttcacgatgc cgctcggcct cctcgccgtc gggtcgcacc
    25741 tcgaccggtc gcgctacgag gtcgtcctcg tcgacgggcg gctcgagacg gaccccgccg
    25801 cggccgtcct cgccgcgatc gacggggagg tcgacggcgc cgccccgttc gtcctcggcg
    25861 tctcggtcct cacggggtcg ccgatccagg acgcgctcga cgtctcccgc gccgcgaagg
    25921 cgcgctttcc gggtttgacg gtcgtctggg gcggctggca cccgtcgctc ttcccggaag
    25981 ggtgcctcga ggaaccgtcc gtcgacgtct ccgtccaggc gcagggggag gagacgtttg
    26041 cggagatcgt ccggcgcctc gacgcgggag agagcctcga ggggtgcgcc ggctgcacgc
    26101 accgcgacga gggcggcgcg ccgcggcgcg aggcggcgcg cgccctcgcc ccgctcaccg
    26161 acctcgcgtc gcacgactac ggcctcttcc ccgtcgagcg cttcttcgag cggaagggga
    26221 agcggcagat cgacgtcgtc acgtcgcagg gatgccgctt ccgctgcgcg ttctgcgccg
    26281 acccgttcgt cttcgggcgg cagtgggtcg ggctgccgcc cgagcgcgtc ggagaggagc
    26341 tcgagagcct ccaccgccgg ttcgcgttca cggacgtgaa cttccaggac gagacgttct
    26401 tcacgtcgct cgaccgcgtc gccgggatcg ccgaggagct gatccggcgg cggctgccgt
    26461 cgacctgggc cgcgacgatg cgcgccgacc agggcgaccg gctccccgag gacgcctggg
    26521 ccgcgtgcaa gcggtcgggc cttcggcgcc tcctcatcgg cgtcgaggcg gggacgcagg
    26581 agatggtcga caggatccgg aaggacatcc gcctcgagca ggtcctgcgg tgcgccgagc
    26641 gctgcctccg ccacggcatc gccgcgcagt tcccgttcat cgtctgcttc cccggcgaga
    26701 gcgacgcgag cgtcgaggcg acgctcgact tcgcccgccg cctcgcggcg atgagcccca
    26761 cgttccagac gcccgtcttc ttcttcaagc cctatcccgg cacgccgctc accgaggacg
    26821 ccgtgaagca cggcttccgc ctccccgcga cgctcgacga gtgggcccgg ttcgacttct
    26881 acgcggggga cggtccgttc acgactcccg cgcggacgcg cctcgtcgag cgcttccgct
    26941 ggtacgagaa gcacgcctac ggcgcggccc cgccctggaa gcgtcccgtc gccgccctcg
    27001 cccgctggcg ctgcgagcgg cgagcgttcc gcttcccggt ggagaaggcc gtcggcgagc
    27061 tcctcttccc ggagccggtc ctctcgtgag ccgcctcctc gtcactccgc cgcggaggaa
    27121 aggcccggct cgagcgcgga ggcctcttcg gtgaagagcc tcgcgtcgac gaccttcggc
    27181 acgccgtcga ggaggacgtg ccgcaccggg accccggccg cctcgagcca cgggcggagg
    27241 tccgcgtcgg cgacccgagg cacgcctccg aggacgaccg cgcggagccg cgccctcggg
    27301 aggtccgtca gcgcccccgc cgggtcgccc ccctcgtcgc gaacgacgac gacgtccgcg
    27361 acggctccgg ccgagaggtg cccggcgtcg ggccgcccga ggacggcggc ggcggcggtc
    27421 gtcacgagtc gcaggagctc ttcgggctcg agcccggcgc tctcccgcgc gacgcccagc
    27481 tctgcgagga ggtcgctcgc gccgctcagc cgcgagtcgc tcccgagggc gagccgcccg
    27541 acggccagga gcgctcgcgg gtcgagcgtc gcgccgagga cgcgcaggtt gctcgccggg
    27601 caccagacga cggccgcgcc ccgctcgatc acgcgcgcga cgcccgcctc cccgaggccg
    27661 acgccgtgaa cgaggacggt cgccgggccg aggccgccga ggcgctcgag gcgggagagc
    27721 tctcccgccg cctccgcgtc ggtcccttcc gccaggtgga cgaagaagcg cgccccggcc
    27781 ggcgtcgccg cgagcgcctc cgcgaccgtc ggcccgtagc gccccgcgaa ccgcagcgag
    27841 tggcaccagc cgtaggggtg gacgacgcgg accgggaacg acgggtcccc catctccgcg
    27901 agccacgggt cgtggtgcgc cacggtcgtg acgccggaga ggaggttctt cagcgcgccg
    27961 tgccgcgcgc ggagcgcctc gtcgacccgc ttcacctcac ggaacgaatc ggacgcgagg
    28021 agcggctgga gcgcgtcgat ccagtcgtac gcgctcgcgc gggggccgcc ggcgtccgcc
    28081 tcggggaacg cgttcaggcc gaggtgctcg tgggcgttca ggagccccgg gaagaggaga
    28141 tgcccgtcga gcggcacgac gaggtcgcca gcgcgcccga tcgcctcgcg cgacacgcgg
    28201 ccctcttcga gccggacgtc cgcccggcgc tcgccctcgg ggccgacgac gcgcgccccg
    28261 gtgaggaggg tcggcggagc gtgtgggtcc atcgcgttcc cggcgtcctc gcgtcaggcg
    28321 cagcggaagg cgagcgcgag gaggacgggg aggtcgcgct gcgcctccca cgccgcgagc
    28381 ttccccgccg cctcgaagcg ggcccgcacc tcttcgccgg ccggcacctc gaggagcgtc
    28441 tcgagcgcga ggcccgcgtc ggcggcggcg cggcggtgcg cgtcgacctc gtggacgtgg
    28501 tgctcgaccg cgaggagctc ccctccgctc cggaaggagc ggacgaggcc gcggcgcgcg
    28561 gcgtcggggt ggaagtcggt cacgaggagg cggccgcccg gcgcgagcgc ccggccgagc
    28621 tctgcgtaga cgcgtcccgc ctccgcgacg tgcccgatcg cgaggcggca ccaggcggtg
    28681 cggaaggagc ccgcggcgaa cgggagcgcc gtcaggtccg cggcggcgac cgcggcgccg
    28741 cgcgcccccg cgaggagcat cgggaggacg aggtcgatcc cgaacgcgcc gcgccggaac
    28801 gcgccgtccg cgctccggag cctgcggccg gtgccgcacg ccgcgtcgag aagcggaccg
    28861 gccggcgacg ggtcgaggcg gcggaccgcg agctcgtcga gctccgcgag agcccccgcc
    28921 tcctcgtacg tcggcgccca gcggaggtac gcctccgcgg gagcgaggac cttgatcggc
    28981 acgggcgcga gtataggagc gggcttccgc ccgcgtggac gcggtcgagg acggggacgg
    29041 ggccgtgacg ctcccgctcg tcctcctcgt caacccccgc gtcgtgcgcc gacgcaacgc
    29101 gaggctcccg ctctcgctcc tgacgctcgc cgccgggctc gaggggaagg cgcgcgtgcg
    29161 gatcgtcgac gggaacttcg acgacgacgc gcgcgcgacc gtcctcgccg ccctcgccgc
    29221 agaacgcccg gcgctcctcg gcgtcaccgt gatgccgggt ccgcaggtcg ccccggcgat
    29281 cgcgctctcg aaggcggtcc gcgagcgcgc cccgggggtg ccgatcgtct ggggcggcta
    29341 cttcccgacg ctctaccccg acgccgcgct cgccgcgccc tacgtcgacg tcgtcgtccg
    29401 cgggccgggc gaggcggcgc tcgtcgacct cgtccgcgcg ttcggctccg gcgatcgcgc
    29461 ccccgcgctc gccgggatcg agggaatcag ctggaaagac ggggaccacg tcgtccacgg
    29521 cgcggaacgc ccgctccggc cccccgacgt cctgccgccg ctcccgtacg agctcctcgg
    29581 ggacgtgccg cgcttcctcc agccgacggt cctcgggcag aggaccgcgg tccaccaggc
    29641 cgcgatcggc tgccggcacc gctgctcctt ctgcggcgtc gtgtcgatgt tcgacgggaa
    29701 gacgctggtg ccgccggccg agcgggtcgc gcgcgacctc ctcgtcctga aggagcgcca
    29761 cggcgccgac gccgtccagt tctacgacca caacttcttc gaccgcgagg agacgacggt
    29821 cgcgctggcc gaggcgctcg cccccgtcgg ccttccctac tggtgctacg cgaggacgga
    29881 cgccctcgcg cggttcccgg cgcggacctg ggagctcgtc agacgctccg gcctgcggat
    29941 ggtcttcatg ggagcggagg cggcgagcga cgaggcgctc cggcggatgc acaagggggc
    30001 ccgcgtcgag gagacgctcg aggcgacgcg gcgctgccgc gaggcgggga tcgtccccga
    30061 gctctcgttc gtcctcggcg ggcccggcgg ggacgaggcg gagatcgaga cgacgctccg
    30121 cttcatccgg aagctgaagg cgatccaccc cgcctgcgag gtcatcctct acttctggac
    30181 cccgacgccg cggcgccggc gcgccgagtg ccccgacctc gccgccgagg cccctgccca
    30241 cgagccggac ccggggaagg cccgcgcagc accggatcgc ctcgacctgc ccgcgacgcc
    30301 cgaggagtgg gcggagccgg aatgggtcga cttcgtctgc caccggaacg ccccgtggct
    30361 ctccccacgg atgcggcggc ggatcgacga cttcgcgacg gtcctcggct gccggttccc
    30421 gacggtgcag gacgtccggc tcccgcgctg gggaaaggcg gtcctctccg ccgcggcctc
    30481 ctggcgctgg gcggccggcg cctacggccg cccagccgag ctgtacgccc tccgacgcgt
    30541 gctcccgctg aagcgccccg aggcggaggg gctgtgagcg cggaggcggc gtcgccgcac
    30601 gttgtcctcg tcaacctcgc gctcgtcccg ggccttcgcg ccggcgagct cctcgcccgc
    30661 ctgtcgggcc tcggccgctt cgcggcctcg ctcgcggagg cgggcgcccg cgtcagcgtc
    30721 gtcgcgcgtt cgcacgagga cgccgacctc actgtcggca gcgtgcggct cgtgacggtg
    30781 agcgacggcg aggggccgtt cgcgccgctg cgcagccccg cgcgtcccgt cgtcgcgcgg
    30841 gtccgggagc tcgcccccga cgtcgtccac gtcggcggct tcctctttcc cgtcccaacc
    30901 gcgacgctcc gagccgccct gccgcgccgg gtgccgctcg tcctccagca tcacggggag
    30961 ccgccaggca gcgggcgcct cggcttcctc cagcgcctcc tgctccgcac cgccgacgcg
    31021 gtcctcatca cgagccgcct gatcgtcgac gagtgggtcc ggggcgggta cctcgccccg
    31081 gagacgcacg tcgaggaggt gctcgaggca tcgaccgacc ttcggcccgt cgagcgcgcc
    31141 gcggcgcgct cgacgacggg gatcgacggc gatccggcga tcctctgggt gggccgcctc
    31201 cacccgcgca aggaccccgt caccgccctc cgcgcgttcg aggccgcgct cgcgcggctg
    31261 ccgggggcgc gcctccacct cgtctacggc gaggcgcccc tgctccccga gctggacgcg
    31321 ctctgcgcgc gaagcccggc gctcgcgcgc gccgtccgct tcgtcggaga ggtcccgcac
    31381 gagcagcttc ccgcctggta ctcggacgcg gacctcttcc tgacctcgag cccgtccgaa
    31441 ggctcgaact gggcgctcat cgaggcgacg gcctgcggcc tcccggcggc ctgctccgac
    31501 atcccggcga accgccgcgt cgccgggccg accgcggcgt acttcccgcc gggcgacgtt
    31561 ccctccgcgg cggaggcgat cgtccggcgg agccgcgagc ccgggccgcg gggcgcggcg
    31621 ctgcgtggcc gcttcgagcg gcgcctctcg tggagcgtcg tcgcgcgcga ggcgctgggc
    31681 gcctatgaac gcgcgatcgt gcggcggggg ggccggtgag ggtcgcgctc ctcgtcccgg
    31741 gcggcgtcga ccggtcgctc accgagcgcg tcatccccgc gctcctctgg ctgatcgaag
    31801 agctcggacg ccgcgcggag ctcgtcgtct tcgcgctcgg gcacggcccg gcgcccggca
    31861 cgtgggaggt cccgggggcg cgcgtcgtcg acctcggacc gctgcggctc tcgcgcgttc
    31921 ccggcctcgc gttcgcgcgg cgcgcgcgcg acctgggcac gctcctccgc gcgcacgggc
    31981 cgttcgacgc cgtccacgcg ttctgggcga ctccgtgcgg cgcgacggct ctcgccgcgg
    32041 cgccccgcct gccgctcgtc gtctccctcg ccggcgggga gctcgcgggg atcgccgaga
    32101 tcggctacgg ctgcgacctc gtcccgcgcg agcggcggaa ggtccgcctc acgctcgccc
    32161 gcgccgcggc cgtgacggcc gcgagccgcc cgctcgtcgc cgaggcgctc cggcgcggcg
    32221 tcgacgcacg cctcgtcccg ctcggggtcg aggacgcggg cttccttccg ccggccgagc
    32281 gcccggcggg cggcccgttc cgcctcctcc acgccgccga cctcaaccgc gtcaaggaca
    32341 cgccgacgct cctccgcgcc ttgaggcgcc tcctcgaccg cggcctcccg gtgacgctcg
    32401 acgtcgcggg cgaggacacg ctcggcggcg ccgtccaggc cgaggcggcg cgcctcggcc
    32461 tcgcggagac cgtcgtgttc cacggccgcc tcccgacggc ccgcctccgc ccgctcctcg
    32521 ccgccgcgga cctcttcctc ctctcgtcgc ggcacgaggc cgggccggtc gcggtcgtgg
    32581 aggccgccgc ctgcgccgtg ccgacggtcg ggacggccgt cgggcacgtc gccgagggag
    32641 accccgagcg gtcgctcgcg gtgccggtcg gcgacgacgc ggcgctcgcc gaggccgccg
    32701 cctcgctcct cctcgacccc gggcggcggc acgcgatggg cgaggccgcg cgcgcctggg
    32761 cccgggagaa ccgcgcttcg gtcgccgcgg agcgcttcgc cgagctctac cgcgaggtca
    32821 ttgacgcgcg agccggcgcg cgccggaagc gagccgccgc gtgaggacgc ccgcccacgc
    32881 cgccacgagc cgtgtgcccg aagcgtccgg cgcgacgttc gccctcacct cgtcgagcga
    32941 cgggaggagc gtccggagga ggaagcgcgc ggcgcggagc cttccgtcgg cgaggccgag
    33001 cgccgtcgcc gtccagccgc gcgccggacg ggagtggcga agggcgggaa gagcggccgc
    33061 gcgctcgagg gcccgcggcg agacgccccg cgcggcccgc tcgagcgcgc ccggctcgaa
    33121 cgtccccggg aagagccgct cgacggcgcg cgcggcgagc gcgacgggcc cccgctcgcc
    33181 gcgtcgaagc tcggggatcg cgagagggcc gctcgtccgc gcgagggcga cgaagtcgta
    33241 tgcctgcacg ccgcggagcc ccttcgccgc gaagtcctcg gccgcgtgga agaggaggtg
    33301 gcggaggagc gccgtccgcg acggaagcgc ccaggctccc gcgcggatcg cgtcgcgccg
    33361 gagcgcgtcc gtcgcgtcga ggacgctccc gaggacctcc agccggaacg agcggtggag
    33421 ctcgatccgg agcgggttcc cggggtgctc gcaggccggg aacgggacgc gctcctcccg
    33481 ctcggcgagg acgaggtgcc ggcgggtgtt ccagagcggc gcgtacccga gcgcgccgac
    33541 gatctccgcc gccgccgccc gcaggtcggc ctccgaggcg aagagaaggt cgaggtcggc
    33601 catcggccgg agccccgccg ggatctcctt cccgaggacg agcgccgccc ccttcagcgg
    33661 gaccgcccgc aggcccgccg acgcgaggag ctcctcgagg cgcgcgagga gcccggtgat
    33721 gcgctctccc cgggctgcgc accgcgccgc ctcgcccgcg aggtggcccc gaagctccgc
    33781 ggggaggacc gtgacgtcgg ggagcgccgc gaggcgcggc ccgagcccct ggagcgacgc
    33841 cgcgaaggcc gctgtccgcg cccgcgcctc gtccgcggcg aaggccgcga gcggcgcggc
    33901 ggagccgtcc ccgagggcgg ccaccgccgc gagccccgcc gccgcgacct cgggctcccc
    33961 gagccgccgc atcgtctcct cgctcgagaa cggcgcggcc tcgtccgtcg cgctcacgtc
    34021 tttcgaaacg ccgtccgcga acgcctcgac gccgcagcgc cggcgtgcgc gttccttcgc
    34081 cgctccgcga tgatccgaga ggaggtcccg ccatgagccc gtcgctcccg cttcaccccg
    34141 ccgtcatcca cttcccgatc gccgcgatgt tcttcgccgc ggcggcgctc gcgctcgcgc
    34201 acgcccgccc gggggcgcgc gccgcgtcgc tctcggccgc ggcgctcctc ctcgccgtcg
    34261 ccgtcgcggg gggcctcgcg gcgttcgtca ccggctggcg ctgggccgac gagctcgcct
    34321 acctcgcggg agggatcgga ccgatccccg gcccgaaggc ggtcgaaggg ctcgcccagc
    34381 ggcacgcgct cctcggcctc gcgtccgtcg tgaccgcggc ggtcgccctc gcgctcgtcg
    34441 tccgcgcccg ccggcgcgag gcgccgccga cgctggctct cctcgcggcg ctcctcgcga
    34501 gcgccctcgt cggggcgacg gggcacgtcg gggggacgat ggtccacaac ccgccgcccc
    34561 ccgccgccga gagcgacgcc ggcgagcgct gacatgctgc tgcgggctct cccctacttc
    34621 ggggcgaggg ctcgcacctc ggcgacgagc gccttgacct gctcgtcgct gagcttccgg
    34681 ccgaacgcgg gcatcttgtc ccggccgcgt cggatcgagc cctcgaggcg cgagtcggag
    34741 accgaggcga gccaccgcgc atcggtcagg ttccgcgcgt tccgcttctt cccctcctcc
    34801 gtgttcgact tcccgtccga cccatggcag acggtgcagt aggactccca ggtcgacgag
    34861 cggcccggcc cctcggcccg catcggaccc gcgaggaggc aggtgccgag cacggcggcg
    34921 gcgattccgg tcattctcga cattcgcgtt ctctcccttc gcgaggagtg tcgcgcgtcg
    34981 cgtcccgctc ctcgcacgcc cgaagcggga aacccgcgac gtcgtgatcc gggtcacggg
    35041 cggcgcgcgc gctccctccg aagaatcggg ccacccgaaa ggagacgcgt catgtccgct
    35101 cgagcccgac ttcatcggct cgctccgctc gtcgtctttg cggccgtctc ggctctcgcg
    35161 tgcggcacgc gcaagagccc gacggagccg gtcgacgacc ctcccaaccc gaacgcgacg
    35221 ttctccaggg tgcaggccga ggtcttcacg ccctcgtgcg ccctctcggg atgccatgcc
    35281 ggcgcggccc ccgcacaggg tatgaacttg agcgcgggga gcgcctgggc ccagatcgtc
    35341 ggcgtccccg ccgtcggctc ttcgcggctg cgcgtcgcgc cgggcgacgt cgcggggtcg
    35401 tacctcgtct cgaagctccg gggggacgcc acgatcaccg gcagccgaat gcccctcggc
    35461 ggcccctacc tctcctccga ccgggagcag ctcgtcgtcg actggatccg gcgcggcgct
    35521 ccgaacgact gaggtccccc atgaagagaa acgcgctgct cctcgccctc ctcctcctcg
    35581 ccgtcccgcc ggccgccgct caggaagcgc ccgcctggga cccgggctac gggatgcgct
    35641 tcctttcgac gcactccccg ctcgtgaacg acaccgggat cctcgagacg ctcttcaccc
    35701 accgcttcaa ccagcccgtg aacgaggccg gcggcaacga cctcttcggc ctcgacaacg
    35761 gcgcgaacat cggcatcggc ctctcctggg tcccggtccg tcgcgtcgcg gcggaggtct
    35821 atcgcgcctc ctccggaggc gactacgaga tcgccgcgaa ggtggccgtc ctcgagccga
    35881 cgggggagag gcccctcggc atcgccgtcc gcgccggcgc gagctggatg acgaagtacg
    35941 acatcgaccg gaaggccggc gcgttcggcc agctcctcgt ggcgtggacc gtcgcggacc
    36001 gcgtcacgat cgccgcggct ccctcgtacg cctcgaacac gcccctcttc gaggacgtct
    36061 tcaacgtccc gctcgcgctg caggtccggc tcgggcgagg cctcttcgcg acgggcgaat
    36121 acgtcttccg caaccgggac ctcgacggct cgaaggggca gtggtcgttc ggcctggaga
    36181 agtcggtcta ccgccaccgc ttcctcctct ggatcgggaa ctccgccgcg acgacggtcg
    36241 accagacgct ggcgggcgac tacgcgggcg gggtgatgga gtcgaacctc cgcctcggct
    36301 tcaacatctc gaggcagttc cggatcgggg gcgactgagg gcgggccccc gccgactcgc
    36361 tctctcgtgc gccccggaca tggagatggc gaggaagcgc tcgggtcctc tcgctgcggc
    36421 aacgacctcc tcggctactt cgtccggaac gacggcgcga cgaacgacgg gacggtcgtg
    36481 ccgttcgagg agcccgacgt ccttccgggc cggcgcacgc ggttcctgcc ggccgtcgtc
    36541 ggcgtgacct cggagcacgg tcggtaccgg accgagctga cgctcggccg gcgggagaga
    36601 tgggggctgc cggagaaggg gctcaccttc accgtcacct accgcgacga gaagggcgca
    36661 acgacgttcc cggtcgcggt cgacatcgac ggcatcgtgg aggttccgga tgccggggcg
    36721 tggctcgccg cgaacggcgt tccgatcgat ccgaacgact tcgcaggaac tctcacgttc
    36781 tcctccgatc ggccggaggg agcgaccgac ctcctcgtca ccgcggccgt ccaggcgcgg
    36841 ggcgccgacg ccgcggggga ttacggcgtg agcgtccacg tcgtcaacga ggtccggtgg
    36901 gcgaaggaga gcgccctcgt ccccgggctc cggcaggacg cttcgttccg ttcgaacgtc
    36961 gccgtcacga atcccgagcc cgagggcggc ccggtcgtga ctctggaggt gacgcttcac
    37021 caggcctccg acggatcggt catcggggtt cttccgcagg tcacgcttcc acccggccgg
    37081 cggttccagt ggaaccggcc tctctcgcag atcggctacg gcggcgacgc gtgggccgag
    37141 gtgcggcggg tcggaggctc cggccggttc gtcgcgtacg gagtcgtcaa cgacaacgtg
    37201 acctccgatg gcacgctgct gccgatgacg gcggagaggt gactgcccgg gaggcgccgc
    37261 tgacgacgat ctccgcatgc tcgccgcgtt gacgggtggg gaccccgttc ctatgatcgc
    37321 ccgcgcgagg caggcgccgc gcgatgaacc gagcgaagac ctccgtcccg tcgcgacccc
    37381 atcacggaag gaacgaccgg gcggagacgc gcgccgtcac ccgcgtcggc gcgagtcgcg
    37441 cggaccggct ttcggggcgg agagcgcgcg catgacccgg cgagtcctcg cccttctcgt
    37501 cctgctcgtc ttcgtcgccg gtccggccct cgcgacggcg gaggaattgc ggcactcctc
    37561 cgcgagaagc ggtccggcgg ccgtggcgga ttcgtgcccc gacccgggga cggacgggca
    37621 gccctgtgga ccggcctgcc tctgcttctg ctgcccgggc cacgccaacg gcctcgcggt
    37681 cttcacgccc cggcccgccg tcgggattcc tccctcgagc gagttcgagc tcgaagcgca
    37741 cgacgacctt cgcccgaagg gcgtcctccg tcgcatcttt caccctcctc gcgcctgagt
    37801 ttctcctccc gagagacggc cgggacgccg cgcgcggtcc cggccccggc gcagcagtga
    37861 cgccccggac gggcgtcctc ggaaagcagg agctcgaagg tgcaaccttc atccctacgg
    37921 aagttccatc ggtggcacgt ccacctcctc tgggtcgtcc tcgcttcggc aggtccggcg
    37981 tccgccttct ccgatccgcc gacaccggcc gatgcagccg gcgcggtttc cgcgtccgac
    38041 tccccggcgg tcctcgagct gacctggccg gacgtcgtcc ggctcgccga gggacatcca
    38101 cggctcgcgg cggatcggct ccaggtcgcg gcggcccgca gcgggatcgc cggggccggc
    38161 gcggtgccga accccacgtt cgaggcggat ctcggccggg gccgcgcacg gatcggcgac
    38221 gaagcgcgcc cggagagcga gctggcgctg agcattcccc tcgggtggat cgccgaacgc
    38281 ggaccgaagc tcgaggctgc cgtcgccgag gcggacgtcg ctctcgccga gagcctcgcc
    38341 tcccggcggg agctcctgct tcagctccgg actctcttct ggaatctggc ctacgagcag
    38401 gcccgggtcg gcgtgctcga gacgctcgtg gcacagaccg cgaagctcac gcggaccgtc
    38461 cagcgacgcg tcgagaccgg agaggtccgc cccgtcgaag ccccgctggt cgagatcgag
    38521 ctcgagaagg tcacggctga gctcgaggcc gccgggacgg tcctccgggc ccggcaatcg
    38581 gcgctcgccc gatggctcgc cgtttcgtca gaccgaaccg tcgttgcgaa ggtcgatctc
    38641 tctgctcttc ccgacgtccc ggaccgcgaa accgccctcg cgcgagcgag gtcggccgac
    38701 ccggcgctga gggtcgcggg cgcgcgctcg cggctccaat cggccgaagt ccgggccgag
    38761 cgcagggcca cgatcccgtc cttctccctt cgggggttcg tcacggacga gctcgaccga
    38821 aaggctcacg gcttcggcat ctccgtcgac gtcccgatct ggagctggaa cagggggcgc
    38881 atcgcccggg ccgaggcgaa gctcgcagcc gccggccggc aggccgaagc gacggcgctc
    38941 gaggtcgaga cgaccgtcat cgaggcccag gccgcgtgcg gcgcgtccgt cgcgagcgcg
    39001 agacgcttcc gggacgagat cgtcccgcgc tccgaacgcg tcgcctccac catggaacga
    39061 acgtacgagc tgggcgaggc ggacctcctc ccggtgatcg acgctcgccg atcgctgctc
    39121 gactcacgcc gcctctccct gaacgcgctc gcgcaggcgc agctcgactg cagccgcctc
    39181 gacgccctgg ccggagaaga gaccccatga acggacgcat cctgacctgc atcctcctcg
    39241 cggccatcgc cccggcggcg gtcacgtcct gccggagggc cggctccgcg ccggccggcg
    39301 acgcccacga gacccattcg gaagagagcc acgcggcgaa cgccgaaccc gcggagccgt
    39361 cggatctcga ccgcccggtc gaggagctcg tcgccctcac ctgcgagcac gagaagaaga
    39421 cgttcgagtg cgacgagtgc cggtacgagg cgggcttcgt gcgcgcgccg gcgagcctcg
    39481 tcgcgggcgg gctcatctcg accgtacggg ccgggcgcca gaaggtcgcc gtccccgtca
    39541 cgctcacggg tgaggttcgc ttcgacgagc gcctcgtcgg tcacgtcagc tcgccggtgg
    39601 aggggatcat ccgggcgacg cacgtgacgc tgggcgaccg ggtgaggaaa ggccagccgc
    39661 tcgtcgagat cgagagcgtc gcggtcggag aggcccaggc ggcctggctc gaggcccgaa
    39721 gcctcctctc gctggctcgc cgcaacttcg agcggctctc cgcgctgcgc gaggagaaca
    39781 tctcttccga gaaagagact ctccaggccc ggcaggagct cgagaccgcg gagatccgcg
    39841 ccgcgaatgc gctcgggacg ctgacccgcc tcggcgtcgg ctccgccgac gcgcgcaccc
    39901 tcacgagcgc caccgccggc ggacgcatcg tcctgcgctc cccgatggac ggcctggtcc
    39961 tgaccctgca cgccgtgccg ggcgagacgg cccggaccga agaggccctc gccacggtcg
    40021 gagacaactc gacggtctgg gtctgggccg acctctacga gcgggacatc cgctccgtcg
    40081 tgaggggaca ggcctcgggg cggctcgagg cctcggtctc ggtcaaggcg tacccggacg
    40141 aggtgttcgc gggcgccgtc gacctcgtca cccaggcgat ggacgagtcc tcccggaccg
    40201 tgaaggtccg cgtcgaggtc cggaaccggg cggggcacct cctctcgggg atgttcgcca
    40261 gcgtccggct gtacctcccg ggcgccgacg aggcgctcgc ggtccccgga agcgccgtcc
    40321 tcgaggacga ggggcgttcc ttcgtcttcg tccaccacga gggcgacgac tacgtccgtc
    40381 gtcccgtcac ggtcgggcgg acctgggccg ggtgggccga ggtcacgagc ggcctttcgc
    40441 cgggccaggt cgtcgttgcc gagggggcct tcctcatgaa gtccgacgtg ctccgctcga
    40501 agatgggcgc gggctgcgcg gactgaggga gcgagaagac gtgggattcg tcgacgtcct
    40561 cctgaggagc cggctcctcg tcctcctcgg caccgcgctc ctcgccgccc tgggcgtcgt
    40621 cgcctggacg cgcctcccca tcgacgcctt tcccgacgtg acgaacaccc aggtcatgat
    40681 cctctcgaag gcgccggggc tcgccgccgt cgacgtcgag cagcgggtga gctatcccgt
    40741 cgagcaggtg atgcgcggcc tgccgcgcgt ccagcaggtc cggtccctgt cgaaggccgg
    40801 cctctcccag gtcgtcatcg tcttcgagga cggcgtcgac acgtactgga cgcgccaggt
    40861 cgtcttcgag cggctcagca ccgcccgcga gcagcttccg ccgggcgtcg agccggagct
    40921 cgggccgatc tcgaccggcc tcggggagat ctaccagtac accctcgagg gggacggccg
    40981 gagcgcgatg gacctccgga cgatccagga ctggatcgtc tccccgctcc tcaagccgat
    41041 ccccggcgtg aacgaggtca acagcttcgg cggcgaggtg aagcagtacc aggtcctcgt
    41101 ctcgccggag aagctcctca agtacgacct gacgatcggc gaggtcgtcg aggcggtcga
    41161 acaggggaac gcgaacgcgg gcgggggcgt cctcgtccgg ggctgggagc aggtctacct
    41221 gcggggagtc gggctcctga aggacgtccc cgacatcgag cgcacggtcc tcaaggccgt
    41281 cgacggctct cccgtctacg tgcgggacgt cgccgacgtc gtcatcgggg cggagccccg
    41341 ccagggcgcc gtgtcccgcg acggcaaggg cgaggtcgtc gcgggcatga tcatcatgct
    41401 caagggcgcg aactcgcagg acgtcgtgag gcgcgtcaag gcctccgtgg agaaggtgca
    41461 ggagacgctt ccggcgggag tgcggctgaa cgtcttctac gaccgcacct cgctcatcga
    41521 ggcctgtatc gcgaccgtcg tcgacgcgct cctcgagggc ggcatcttcg tgatcctggt
    41581 cctcttcctc ttcgtcgccg agctccgtac ggcgctgatc gtcgtcctct ccctccctct
    41641 caccttcctc gtcagcttca tcgtcatggg cggggccggg atcacgtcga acctcatgag
    41701 cctgggcggc ctcgcgttct cggtcggaat ggtcgtcgac gcttccatcg tcgtcgtcga
    41761 gaacgtccgc cgtcacctcg ctcacgaggc ggcgaaggag cacaagcggc gtgtcgtcgc
    41821 cgcggcagtc gccgaggtcg cgaagccggt cgccttctcc gtgatcatca tcgcgatcat
    41881 cctggtgccc ctcttcacgc tccagggggt cgaggggaag atgttcgccc cgctcgcggc
    41941 gacgatgctg atcgccctcc tcgtctcgct cgtggtcgcg ctcacggtcg tccccgttct
    42001 ctccgaggtg ttcctgcggc agctccccga gcgggagttc gcgttcatcc ggcggctccg
    42061 agaggggtac gttcggctcc tcggtcacgc ggtgcgccga cctgcggtga cgctgggcct
    42121 ctcgggcggc gccctcgtcg cctctctggc gctccttccc ttcatgggta cggagttcat
    42181 gccgccgctg gacgaggggg ccatcgcggt gaacgtcgtc cggctgccga acgcctcgct
    42241 cgagggctcg gtcaaggtcg cgaccttcat ggagaagcgc ctgctctcgt tccccgaggt
    42301 cgagaccgtg gtgagcaaga ccggccgggc ggagatctcg gaggacccga tggggcccga
    42361 gcagaccgac gtcttcgtca tgctgaagcc ccgccggaaa tggggcaccg ggcgcgacac
    42421 ggccgagctg gtcgaggcga tgcaggagga cctctccgag atccccggcc tgcgcttctc
    42481 gttctcgcag ccgatcgcgc tgcgggtgaa cgagctcatc tccggtgtga agagcgacct
    42541 cgcggtgaag gccttcggcc ccgacctctc ggtgctgaag acgttcgcgg accgcgcggc
    42601 cgccgagctg acggggatcg agggcgcccg cgacgtgaag gtcgagcagg tctccggcat
    42661 gtcgcagctg gacgtcgtcg tcgaccgcga ggcgctggcc cggtacggca tcaaggtcgg
    42721 agacgtcaac gccacgatcg agacggcggt cgccgggcgg cacgcgacga ccctgatcga
    42781 ggaggagcgc cggttcgccg tcgtcgtccg cttcccggag tcggcccgca cggacgtccc
    42841 cgagctcgag cgtctgctcg tgccggcccc cggcggggag cgggtgccgc tcggacagct
    42901 cgcccggttc accgtcgtcg aagcgcccgc gcaggtgagc cgcgagaacg gcatgcgccg
    42961 ggtggtggcg gaggtcaacg tccggggacg cgacctgggc gggttcgtcg ccgaggcccg
    43021 ggagcggatc gcaccgttcg cgaagcagct cccaccgggc tacaccgtcg agtacggcgg
    43081 ccagttcgag aaccagcagc gcgccatgcg ccagctcgcc gtcgtcgtgc ccgtcgcgct
    43141 gctcctgatc ctggtcctgc tctacctggc gctcgggtcc gtccgggact cccttcttgt
    43201 gctcctcaac ctcccgttcg cgctcgtcgg aggcgtcctc gccgtcgtcg tcttcgggat
    43261 gcccgtctcg gtctccgccg cggtggcgtt catcgtcctc ctgggcatcg cggtgcagaa
    43321 cggcgtcgtc ctcgtcgcct acttccgcca gctccgcgag aagggcgaga ccgtggccga
    43381 cacggtggcg aagggctgcg aggtccgctt ccgcccgctc gtgatgaccg cgctcacgag
    43441 cttcatcggc cacctcccga tgctctacgc gaccggctcg ggcgccgaca tccagaagcc
    43501 gctggccgtc gtcgtcatgg g
//
LOCUS       DAOONL010000021        43323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_641_length_43323_cov_17.248775, whole genome shotgun sequence.
ACCESSION   DAOONL010000021 DAOONL010000000
VERSION     DAOONL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 43323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_43323_cov_17.248775"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLP50_05325"
     CDS             complement(<1..854)
                     /locus_tag="PLP50_05325"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPA51007.1"
                     /translation="MRASLAALLALAIAFPLLAEWPPKGRGAATRSPVWAEHGMVASA
                     QPLCTQAGVETLRKGGSAVDAAIATNACLGLMEPTANGLGGDLFAILWDPAQKKVVGL
                     NASGRAPLALTADKVPPLPDGTIPLFSPYAWSVPGCGDGWLELHARYGKLPLAEVLAP
                     AIRYAEEGFPVSPVIAGNWAGSVNRFKDKPGFAEVFLPGGRAPKEGERFRNPALAKTL
                     RLLATKKRAGWEGEFADAIVAFSKANGGFFEREDFVRHRSTWDAPMSTGYRGYDVWEL
                     PPNTQGLAA"
     gene            921..2945
                     /locus_tag="PLP50_05330"
     CDS             921..2945
                     /locus_tag="PLP50_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51008.1"
                     /translation="MFEAASSSRFQGGLLPRRTFLALLAFASLLGPLLVFRETVRDPL
                     ERIPCGRPVPNERLGDPVIVLSVMGRQWARWDSGDFSRRDDRVFAPYPDTWPLGEPFL
                     LPSAIGYPWSRLADSDALGYNVPYFLATAVACFAAGLLCADLVGPGWAALIGALLLAW
                     SPGRLNNFGVLGTIWAGFVSLVLLFALRYLRSGRVRDAAACAAALLVVGLGSPYPLVM
                     GTVSVAVVAAASARSFRRPVLLGVGGSAAIVLLAVAYAPYLHLARDFDAPVGLRSIEV
                     HAADLLSILHTGVFAGPLRDLLDGLVPVFPEGASALFPTLTALAAFGAGAFLLRRRGP
                     WRRRDARHAARSLAPWLLLAGISFVFALGPTIRFAGRALGPGPYALLAQLPGFSGMRG
                     IHRWDQWFGLGVIVTATLVLGRLLRALPARRGRLLLVGVAPLLVLDLWTRPVPAQPVP
                     APSPFDAELRALPRDAIVAVWPFRRGTSERSWAEQLSHRRRVLNGFQTYPPPIHFWLD
                     SFLSDKPFEAAFAAYSELGVAAIEVDLEAAPRGSGERLGALLASGALPGVRAAMGSRS
                     RLLLLLEPKAPILVDPRTIEGLSFEGDSAVVARAEGRLLFRLGSGRLAVVVESPAGPV
                     SDVLRIPVVGAGELGTRLSRSLPPGAVVRAAADGREIGRTGPPPSRPPSG"
     gene            2957..5524
                     /locus_tag="PLP50_05335"
     CDS             2957..5524
                     /locus_tag="PLP50_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA51009.1"
                     /translation="MRDLEPRQRRIVLFALGAALVVSLAWTTWLAHLGVERGALLRAR
                     LWLALLVFGVHYVGAVVVLLKRPSDPANQALFPFLTLLGVLNAGRILGVVTGGVTFQD
                     RIAWAGSTAVHFVAIAWILVTIGGFFPVTRLHRPGRAYFVGTLVAMAPLAFYLRRWWL
                     DGGAAAPGGAWRTGVAIVALAVDLFVFVYFLSLAWVARKEDVRRRARVVLVAVLLYLG
                     LYIFLRDVPAVAGRSALLPVPVLSTLSVLLPLALIVAATRFRLFEVDRLIRRSASYAI
                     ASGALVVLLLAGVPALTALVALVSPALQGTLTTALVAVLLFLVFDPLRKRAQIALDRK
                     FRQGAYDPAQLVTDVVGDLRDAGEDLAGVVLHRIASAHRPDRQAFWVFALEAPGAAVR
                     PVTTGALADVPGLDRAAVLPMSDPLEARNSFHALTADGGDPPYAEGELANGLRAAGFV
                     AALPLFSGDGEPLGLIALGAKRTGETYVPEEQRILGTVAGHVALALERAALTRQVDED
                     RRVREKVLGHLQEGGSGALYECELCGRVADEDDLVCTADGHAVKMTQAVPRLLAGRYR
                     LDRRLGEGGMGTVYAARDIRGNRDAAVKVIRAEHLADRSAVARFRREARLTARLGHPN
                     AVAVYDYGELRPGGAFLVMELLSGGSLRNELILRKPIPFLETAWALDRALDVLAAAHA
                     AGLVHRDVKPDNLFVTRGPGGATRLKLLDFGLARETKAEGGAESGGAISSASILMGTP
                     GYIAPEIVSGGEATPASDQWAIAATGVELLTGVRLKFDRPDLTLKSLVEEVVSVVTTV
                     ASGVPVTYARSLAKGMAMDPEDRWVSVLALRRALDRSTGGRIPGDGDVEFDVLREET"
     gene            complement(5555..5935)
                     /locus_tag="PLP50_05340"
     CDS             complement(5555..5935)
                     /locus_tag="PLP50_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51010.1"
                     /translation="MRSANGTAPEEDVRPPIRFAGRSFVVASNQPTEVLRALEREIEA
                     RGGSIHPFLNAVTEVLVVGDLAGMDSGAHWAREQVRRGTIYRDRWGHLELISEEELRA
                     ALVESRRGRASGSPFDDRSIADGA"
     gene            complement(6333..7628)
                     /locus_tag="PLP50_05345"
     CDS             complement(6333..7628)
                     /locus_tag="PLP50_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51011.1"
                     /translation="MPSADRHLGELELLERAMTPGLAPDPHAASCPTCRDRIEGLAAG
                     REHLADLAALATKAESGPRVPVPAALSRLSRTLARAEELVRAAERDDDALPAWLDEAS
                     ARPDFPDVALHALQIASRLTSRRPSVALSFARSIRQRLHGDVGGDQALLAAEIGLLES
                     QSFVYVGRIDEACERALAASSAIEAADAPALLRARANYYAGSALWGAGRSEAALSRLL
                     AAREAFAADGQDPWVGRAEGAIGLVHFSEARFREALHAFDAALERLDPDVDPGPWEAI
                     QQNRAGILMNLGRLSEARSAFGQALELAVRHGLSAAATTVRVNLLNLGLEEGTFEDVR
                     VRGKKLVAFCAREGLSVDAYYARLALAEAQAALGNYGEVRALVEAIRGDAPPEVRDDP
                     DATVLLDRLDAGDHEMAGRLRRLRHYLSGRDRFEAAKRA"
     gene            complement(7615..8175)
                     /locus_tag="PLP50_05350"
     CDS             complement(7615..8175)
                     /locus_tag="PLP50_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51012.1"
                     /translation="MPGETAPLDNVAWAASRFATPKEAERPAGLEPSYWDWEDLYSRL
                     GPGLIVLGARRFGLSRDDAEEALQMAATAIVLAAPSVRSPEAYLTSVFLRECLGILRR
                     RSQLARTEVAPPVGFDAADDSCERIEVACRFRQAFSLLSPVCRSMMRSCLLGGRSRAG
                     SDSSSAARTVYKRYRKCLRTLAHALG"
     gene            complement(8183..9253)
                     /locus_tag="PLP50_05355"
     CDS             complement(8183..9253)
                     /locus_tag="PLP50_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HPA51013.1"
                     /translation="MSIEVRSVSKSFGSFLALDDVSLRVGDGELVALLGPSGGGKTSL
                     LRIIAGLEEPDRGTVLFHGEDATRRDARERRVGFVFQHYALFRRMTVFENVAFGLRVK
                     PRGERPSEADVKRRVGELLKLVQLDWLGDRLPSELSGGQRQRVALARALAVEPKVLLL
                     DEPFGSLDARVRQELRRWLRRLHDEIRLTSLFVTHDQEEALEVADRVVLLDRGRVVQD
                     GTPADLFEHPADPFVLDFLGSVNIFHGRVENGRAHLGPLSVAYPDAPHDGALPASGYA
                     RPWEIDLSRDLEGVEGFPATVRHVAAAGAVVRLELAPDDGTILKVEISRERFAELAPS
                     PGDRLRVVPRNVRVFLGGPGQG"
     gene            complement(9250..10077)
                     /gene="cysW"
                     /locus_tag="PLP50_05360"
     CDS             complement(9250..10077)
                     /gene="cysW"
                     /locus_tag="PLP50_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HPA51014.1"
                     /translation="MTAPRSREPRAVRLLLIGLALAAFALFLFVPLAAVFVEAFRDGA
                     RAWLAAVTEPDARHALKLTLLVAVIAVPVNAFFGLAAGWALGKFRFPGRSFVLTLVDL
                     PFAVSPVVAGLVFVLLFGRTGFFGPWLADRGLEIVFALPGIVLATVFVTFPFVAREVV
                     PLMQSQGTDEEEAARVLGAGFFTTFFRVTLPNVKWALLYGIVLCTARAVGEFGAVSVV
                     SGHVRGETNTLPLHVETLYNEYAFQGAFAAASLLTFVALATMVAKAIVSRRAREEAP"
     gene            complement(10074..10904)
                     /gene="cysT"
                     /locus_tag="PLP50_05365"
     CDS             complement(10074..10904)
                     /gene="cysT"
                     /locus_tag="PLP50_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HPA51015.1"
                     /translation="MSRRRVSPSLLPGFGPALGVTLLFLSLVVLAPLAALLATTLRGT
                     PREFLAAVSTPRVVAAVRLSVLVSLGAAAVSALLGLLLGWVLVRYRFPGRRLLDTLVD
                     LPFALPTAVAGITLTTLYAPTGWLGRPLAALGVPVAFTPLGIGVALVFLGLPFTVRTL
                     QPVLASLDPEVEEAAASLGATRLETFRRVLLPELLPALLTGSTLAFARGLGEYGSVVF
                     ISGNMPMRTEIAPLLVMTKLEQYDVAGATALATLLLVASFLLLLVLNRLEAWAARAVR
                     "
     gene            complement(10901..11938)
                     /locus_tag="PLP50_05370"
     CDS             complement(10901..11938)
                     /locus_tag="PLP50_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPA51016.1"
                     /translation="MTDSRRPVRSLLGFAASAASLLAIHALAGAAARAAEPVEILNVS
                     YDPTRELYRELNAAFAKDFLARTGRAVTVKMSHGGSGKQARSVIDGLRADVVTLALAY
                     DVDALADRGLVARGWQARLPHNASPYTSTIVFLVRSGNPKRIRDWSDLARPGVSVVCP
                     NPKTSGAARWGYLAAWGSVLTTGGSDAAAKALVGGLFRNVAVLDTGARGSTTTFVQRG
                     LGDVLVAWENEAHLALAETGAGRVEIVVPKTSILAEPPVAVVDAVADRKGTRAVAEAY
                     LRFLYTPEAQAIAARHHYRPRLAEAAAKAGRTFPSVATFTIDALFGGWRAAQKRHFDD
                     GGVFDSLFTTK"
     gene            complement(11938..12396)
                     /locus_tag="PLP50_05375"
     CDS             complement(11938..12396)
                     /locus_tag="PLP50_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPA51017.1"
                     /translation="MKVSQRGLYALKALLHLAGRWEEGIVATREIAEREEIPEKFLES
                     ILVVLKNARVVESRRGREGGHRLRRPPAEIVVGDVVRLLDGPLAPFGDATELTRLVAS
                     EPRHAGLFDLFLDVRNAAAAILDHTTLADLLERDRKILVARRRRGGVPET"
     gene            complement(12592..13383)
                     /locus_tag="PLP50_05380"
     CDS             complement(12592..13383)
                     /locus_tag="PLP50_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51018.1"
                     /translation="MRTRTAAALALAFAASFPAAAATRAGFELSVLVDGEERPELRKG
                     GSVYVEALKGHPYALRVTNPLGVRVGVALAVDGLNTIDASRRDAASAVKWILEPYQSV
                     VLDGWQVSSSHARRFVFTGERDSYGAALGKVEDLGVIEAVFFREKVRPALPVSPTACA
                     PKDAAPRSEASRAKGEAGAPAEKMAAAPSLSDEYAATGMGDRTRHEVTRVDLDLERSP
                     AARLRIRYEFRPQLVRLGLLPPDGDRLARRERSRGFDRYCPEPNR"
     gene            13694..14878
                     /locus_tag="PLP50_05385"
     CDS             13694..14878
                     /locus_tag="PLP50_05385"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPA51019.1"
                     /translation="MNVLVLNCGSSSLKFAVIDPDTSVEHVSGIAQRLGSPEASLDVK
                     RNGTKSTRPIPGALHDEALHGVVDALKELGLADGLLAVGHRVVHGGSKFAGSMAITPE
                     VVAKMEECIPLGPLHNPPNLVGIRIAQSLFPDLLQVGVFDTAFHQTMPPVAYTYPVPF
                     EWLEKHSVRRYGFHGTSHRYVAREALRLAGLPADDHAIVTAHLGNGCSAAAIRNGKSV
                     DTTMGLTPLEGLMMGTRCGSIDPAIIGHVADATGKSAKEILDSLNKKSGLLGVSGLSN
                     DMRTVEEAASNGNDRAKLALDIFCYVLAKTIAALVVPLGRLDALVFTGGIGENSTTVR
                     AMTLGRLGFLGLALDEAANAKHGRAQHGRITTGTKPQALVVPTNEELMIAEDAAQIAR
                     GA"
     gene            14901..17066
                     /gene="pta"
                     /locus_tag="PLP50_05390"
     CDS             14901..17066
                     /gene="pta"
                     /locus_tag="PLP50_05390"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPA51020.1"
                     /translation="MRTTVLVAPAGRGVGLTTACLGIVRALDREGIRVAFAKPVANRE
                     PDHTIPLLSLGAHFAIPPAVPRAEAEGLLADGDDQTLMERVVAIANEAGEDVDVLIVQ
                     GMHPEMGMVHSARVNALMLKALDAELVLVAAPSDQTAAEVAETVAIAARGYGAFSEGR
                     SVGCILNRVCDRRGMPAEPEVDSFGPSAGGCGACAPAGLSETCMKDHHAALEAEGIRP
                     IAIVPCNSDLMSPRVKDIGDALRAQFLYTGEAATRRVRDVAICAMTVPNAMKAMKPGT
                     LVLTPGDRSDILLASAMTVQSGTPLAGVVLTGGLGPDRGVLDLCRKVFDAGLPLLQVK
                     ASSYRAAAQVASMNPTIPIDDADRVERTMSFVADHVDLAWARAFAKPARVPRLSPPAF
                     RHRLVETAKADVKRIVLPEGAEPRTVAAASIVASRGIAKCVLLGNPDEVRDVAGRQGL
                     TLPDSVEIVNPASVAHRYIAPLVERRKSKGMTPERAELELQDAIMVGTMMMALDEADG
                     LVSGAVHTTAHTIRPALQIIKTVPGCNLVSSIFFMCLPDQVLVFGDCAVNPNPTAAEL
                     ADIAIQSADSAVAFGVPARVAMLSYSTGTSGTGEDVEKVKEATRIAKELRSDLALDGP
                     LQYDAAVMPDVARLKAPKSDVAGKATVLIFPDLNTGNVTYKAVQRSANVVSIGPMLQG
                     LAKPVNDLSRGCLVEDIVFTINLTAIQAQQAARARASKG"
     gene            17185..18543
                     /locus_tag="PLP50_05395"
     CDS             17185..18543
                     /locus_tag="PLP50_05395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPA51021.1"
                     /translation="MKNVLARRIAAVLVAAAFGVACVTNPVTGKSQLSLLGEEEEKAL
                     GAQAYGPLIQESYGPVNDPGLQKFLDGVGQGLAKVSHRPALDYEFTVVNANYDNAFAL
                     PGGKICITRGLLSRMTTEDQLAGVVGHEIGHVTARHGAQQYTKQMLIGGLLGIGGMIL
                     EAEEVRGAPLIMAAAGIGGQLVLLRYSRDHERQSDELGMEYMARAGYNPKGFVESMEI
                     LKAGHDTEPSKLEAMFQSHPLTSERIETARRRSAEKYPEQLGRPVRTETFGASTRALK
                     AEAPAFKLMDEGEKLLAKKDARAAAAKFDEAARLAPRQAILPALKAIALVEANELRSA
                     RESAREAARRDPALFHGRLAGGLSAYRLSDWRDAISELETAEKSVGAQLVTTYYLGRS
                     LEALGDRGQAAEKYKLILQSGATGEQADYAKRRLLEWGVISQTPQQQPYPQQQPQQPY
                     PR"
     gene            complement(18625..20442)
                     /locus_tag="PLP50_05400"
     CDS             complement(18625..20442)
                     /locus_tag="PLP50_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPA51022.1"
                     /translation="MRAGTLLGKAEQSLLVAALAVATVLPLIDAFGRPLGGFHVPGSG
                     SILQLVTLWIAFVGGLLATREGTHLTLSTAELLGSGRARRLARLFAFSVAAAVCAVLS
                     WASVGLVKADREQAKVLSFGLPEWIAELVMPAALLLMAIRFAWKASDGARGRFAALAA
                     LASPWLLSLVPEASAPKLVLPLAAVILLAALLGAPVFVAMGGLACLLFWKDAVPVAAV
                     SAEVYRLVASPTLPAIPLLTAAGYILAEGGASARLVRFFRALFGWMPGGIAVMVAAVC
                     ALFTTFTGGSGVTIIALGGLVYPILRDDGYPEGFSLGLVTAAGSLGLLFPPSLPVILY
                     SVVASVPADSLYLAGLVPGFLLVVLTAAWGIRQGLKLEAEGRSRPSFALRELGASFWE
                     AKWELALPVLVVVLFASGFASMVEASAAACAYAAIVECFVTRDIHPFRQLPGVLVKAG
                     ALMGAVLILLSIAMGLTSWLVDAMIPAALLDYVKAHVSSPLVFLLVLNGILLVLGSVL
                     EIYSAIVILAPLVAPMGAAFGIDPIHLGVIFLANLELGFLFPPVGLNLFLSSSRFGIP
                     LPKLYRHAVPYLLILGAGVLLITYVPAMSTGILTLLGKR"
     gene            complement(20439..21479)
                     /gene="dctP"
                     /locus_tag="PLP50_05405"
     CDS             complement(20439..21479)
                     /gene="dctP"
                     /locus_tag="PLP50_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPA51023.1"
                     /translation="MTARSPQAARRRLLLPALALFLAVASPARAQVVVKMATLAPDGS
                     SYHLILKEMAEKWKTATQGRVTVRLYPGSVAGDDADVVRKMRLGSLNAGLLTSSGVSA
                     IDPSVMALQVPMLYASYDEVDHVLAVYGPKLEASIAAKGFVVLNWADAGWVRFFTKTP
                     VRTPSDLKPLKLFAWGNDTDALEIWKGAGFNPVPLPSTEISTALQTGLVSALPTTPTA
                     AVLLQWYQHAKNLTDVKWALLLGATVVSKATWDKISPEDQRALRAAAAEAGARLRSES
                     RAAEARDVAAMQKRGLNVIAIDAKTEALWRAAAEAAYPKVRGKIVPEAAFDEARKILA
                     DFRAKKAAAPKK"
     gene            complement(21486..22415)
                     /locus_tag="PLP50_05410"
     CDS             complement(21486..22415)
                     /locus_tag="PLP50_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HPA51024.1"
                     /translation="MARTRRWAGALSPFLAASLLASGCSVRKVAVNSLANALAAGGDT
                     YATDDDPELVAAAIPFGLKTMEALLAETPKHDGLLLAASSGFTQYAFAFVQAEADFVE
                     EKDLARATALRARARKLYARALQYGLRGLEARHPGFEAGLRDEARRGATLAAATKTDV
                     PLLYWTGAAWGAAISLSKEDAELTADQGLAEALERRALELDEAFGKGSIHDFFISFEG
                     GRPAAAGGSVERARRHLERAVALSDGRRAAPYVSFAETVSVGAQNRPEFEELLQKALA
                     VDPDAVPELRLANTVARRRAAWLLARADELFLE"
     gene            22544..25723
                     /locus_tag="PLP50_05415"
     CDS             22544..25723
                     /locus_tag="PLP50_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51025.1"
                     /translation="MSQRAALRGFFLCILALLFGRPLLGQSGTITFAPDPVQVRVGEI
                     SQVVTATIEYLGTPPGGAQTLSFNGGDGISGATTSPPTITFTPVVGGPTTVLFQLVAA
                     ADAFPGPFEASASTTGAFGLMNYEILPFSVSPDSVLVGAGNTVSGLTATVSYPDFTPT
                     GPQQLTFSGLPAGASPVPSPVSFTVTGTGAVVGFGIATSPATPPGNYTVTVGTTPVAT
                     GTDTFQLLVAPGSSISISPTSTTVLPGETTAELTATVNFGPPGPTGPQQLVFSGLPPG
                     ATSVPSPVSFSIIQIPTVNVPFSIATSAATPPGSYVVTVGTSPASAGTASFELVVAAS
                     GRLDLSLASSSLDLCPGGAAVENAVTVTPADGFEGSAEVTFPLLPAELVVTPLSVPVD
                     AMPPAQTVSFAVAARPDATPGQKVVVVRVASPFGAGALTSFTVNVAAPGFTPALSPEA
                     LTLDAGGGTEKVIVSLAASACPATAETIVVTPSGLPAGVTVTPRSAVLFGPTHPPVEF
                     TFVAASSVPAGTATATFAFEPSSGGARTLPFLITVQRPGRLTVVAERPVMDVCPGGGA
                     APNTLTVSGTDGYTGTPTVTFPGLPAGLTATPATIEVQSVPPARVVSFTLSAAAGLAV
                     GDLTVTVKVADPNGVEATTTVVARVLPPDFVPSVAPASLVLSAGGAAGTMTASLTPGS
                     CPPSTDVTITPSGLPPGVVVTPTSAVLSPPELGPALFTFLAGAGAEPGASTITFTYAS
                     PGASPKTTTATISVCGAPAAPDSPTIRPAGNPQGPVTATDFLDLSWMAPDSGFPATRY
                     DWRINGGPWTAAAGTSAGAPPRGAVDPVQLFVRAFACDPEKGPGPEASSPVYALAAPQ
                     ASFAVPASIVVGQPVTFTDTSSPQATSWLWFPGDGMPATTVQSPTVTFPSAGPRVIVL
                     VATNGSGTSTKSTTVNVLPASAVRTAAGWFARRLDRQPDGRLALDEVDVARGTTLLLR
                     RLSGAGDAVAFLRLVDPTGATVVERRLVLAAGEEARHELSAFGEGSFRIELVGPEGLE
                     ATVVTTAVPTPAPELPAPPREPRRIEPRR"
     gene            complement(25881..26768)
                     /locus_tag="PLP50_05420"
     CDS             complement(25881..26768)
                     /locus_tag="PLP50_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPA51026.1"
                     /translation="MSSVVTTPDPPRFQPGDYKSDLKPVWCAGCGDFGVVSAMYRAMA
                     ELQLEPWNTVVVSGIGCSSRLPGYVEAYGFNSVHGRALPIATGIKVARPELNVVAVGG
                     DGDGLAIGGNHFLHASRRNLDVFYVLMDNEIYGLTKGQAAPTTPSGDKTKSTFFGNPE
                     PSVDPCELAISFGATWVARGFSGDLKGLVDLMVAGMSHRGFAFLNVMSPCVTWRGDDQ
                     FKVMKAKLRGLPEGYDRHSRAAAFAYTRETETLTTGVLYEAHEPSLLDRLEAQRAKAM
                     QGYEELTTEKVLKVFFPPF"
     gene            complement(26765..28540)
                     /locus_tag="PLP50_05425"
     CDS             complement(26765..28540)
                     /locus_tag="PLP50_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPA51027.1"
                     /translation="MPTRDLTLAMVGSGGDGVVTMGEMMAQAGARDGLNVIKVEAYGP
                     QIRGGESSCTLRMSAEPISAQGDAVDVLVVFNWADFTRFKGEVVPKADAVILYEASDP
                     TPLEDVKIPTATNATWIPVPFVELATKATGTKAAKNIVTLGILAELFGIPQESVRKAV
                     EFKFGKKKAGVLEANLKGFAAGREFAGTIGVDVSARKLEYAISAPKLVMTGNEACAVA
                     ALHAGCRFFAGYPITPSSEVLHFLSEWLPRSGGRIVQTEDELSALGAIIGGSFAGVKS
                     MTSTSGPGLSLMAEMLGLASMAEIPVVILDVQRGGPSTGLPTKSEQSDLYMAVFGSHG
                     DAPRIVLAPTDVEDCFHATVDAFNIAEETQLPVIVLSDQAIGQSRQTVEKANLVHEVI
                     DRRTPTAEELADYKRYAQTPTGVSPMSVPGTKGGMYQTNGLEHDETARPASSHVMHEK
                     MSRKRFAKLDFVEEKYPLHVHLGPEKADVGIVCWGSSKGPVEEAMKELAANGVSVSAF
                     VPRIIMPFPKKAFLEYAASVKTLVFLEVNFVGQLYNYMRTFVDLPAGTRVFTRSGGMN
                     LSVSEVKGRIESVLSGSGVREEVTA"
     gene            28720..29529
                     /locus_tag="PLP50_05430"
     CDS             28720..29529
                     /locus_tag="PLP50_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HPA51028.1"
                     /translation="MKALPLRTATAAPILFAALLAAGCSGKEAPQAPATDAGAPLVGA
                     HGGEAAAPAVPSIRGTVLETMDAAGYTYLKLKTAEGETWAAVNEAKVAVGDEVTVLQP
                     MTMDGFESKTLNRTFERIVFGTLGPGTAAPATAPGPAGPVPAAMAAAHAAAADGPETA
                     EKISVAKAEGPEGRTIAELFAVRAALAGKPVAVRGKVVKVNLGIMGKNWLHLRDGSGT
                     AEGKDHDLTVTTQDAVSMGDVVVVKGTVGVDRDFGSGYTYSLLVEDAKVTK"
     gene            complement(29519..30190)
                     /locus_tag="PLP50_05435"
     CDS             complement(29519..30190)
                     /locus_tag="PLP50_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPA51029.1"
                     /translation="MKPALPELTLAVEAAAVVVMTVSGMITAARARMDLIGTFTLALV
                     TAFGGGTLRDLILERRPLFWVANEGYVFGTLLLCLAFVYSRRAYATAAALDRKAVFVD
                     ALGLALFSLTGVRYALEAGLPPLPASLLGVVTGTFGGVLRDVLVREIPAIFRPGALYA
                     ASSFVGCWVVLAGPFLEVRQSFADAAGFATIVLLRMASVHLGLRLPDPHWFGKADGGP
                     DGPTS"
     gene            complement(30187..31383)
                     /locus_tag="PLP50_05440"
     CDS             complement(30187..31383)
                     /locus_tag="PLP50_05440"
                     /inference="COORDINATES: protein motif:HMM:NF017447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51030.1"
                     /translation="MPRRTSPTALLVVVGLAFFADSILYYLIVPLLPHYAKTLGLSQM
                     EVGVLFGVYAGALLAGTYPLGRLADRIGRRRPFLWGLVGLGATTFLFAFSASYPLLLV
                     ARALQGLAAAATWTTGLALLAEGFPSEKRGQAMATVFAAANVGVLLGPPVSGLLTESL
                     GPRSPFLLAGAIVLADAVARVLLLGDDESPRGERVGLRGLLADGTVRTLAGAMALGAA
                     LWAILESTLPLHLDSALRTGPKEIGILFAVAALAHTVTSPVMGRLSDRVGRKKVLLVG
                     YVLAAFLLPVPAFLASKGSVGLAMAALGVTTSFVLSPVSPGLADAVERMGSRSFGGVF
                     SLVNIAYAVGMMGGPLLGSALVEAAGIRGALVATSALFALYLVPLARMAPSPRRSAAP
                     ATIPAP"
     gene            complement(31447..31704)
                     /locus_tag="PLP50_05445"
     CDS             complement(31447..31704)
                     /locus_tag="PLP50_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51031.1"
                     /translation="MPRRTLALLVLAAAPCAVARENPAAPAPPPSATRTFEYVAQVRL
                     RGDTYRDLDLTSERFGPGELAKSVRGDDDALGASASLLFAF"
     gene            31899..32378
                     /gene="nrfH"
                     /locus_tag="PLP50_05450"
     CDS             31899..32378
                     /gene="nrfH"
                     /locus_tag="PLP50_05450"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPA51032.1"
                     /translation="MPSRRGKVRRRGLALAETAAGLLLGTALGVGAYTFVYAKGASYL
                     SNDPNACANCHVMTEQLDGWSRGSHRAVATCNDCHTPPGFVAKYATKASNGFWHSFAF
                     TSGRFPDPIQITDRNHRVTERACRKCHADVVHDIDRVTREGRELSCISCHRSVGHPH"
     gene            32407..33864
                     /locus_tag="PLP50_05455"
     CDS             32407..33864
                     /locus_tag="PLP50_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPA51033.1"
                     /translation="MTEKKSSTARTILVIAAIAAFAAVGVTALLVNIMEHKQEARNPF
                     FRVVELDDDTEDPAIWGKNFPVQYDTYKRTVDMVRTKFGGSEALPRTPTEKDPRTQVT
                     TSKIEEDPRLKRMWLGYAFAVDFREERGHAYMLDDQTFTERQQVVQQPGTCMNCHAST
                     YVAMKKLGNGDIVKGFEAINQMPYFDARKHVKHPVACIDCHDSKTMALRVTRPAFMEG
                     IRAYKASQGVPDYDVNTMATRQEMRTYVCGQCHVEYYFKGAEKRLTFPWHKGLKADEI
                     LAYYDEVGFKDWTHAETGSPNLKAQHPEFEMYNQGIHARSGVACADCHMPYTKVGAMK
                     VSDHHVRSPLLNINKACQTCHRWSEEELKARAETIQSRHMEMRNLAMDALMSLIDDVK
                     AAKEAGKSDEELAVARDFQRRATFLLDFAEAENSTGFHAPQEGARILAQSIDASRKGQ
                     LAVRGALPATPQPLQKAEAPAAPQAPGKVALLSAR"
     gene            33961..34752
                     /locus_tag="PLP50_05460"
     CDS             33961..34752
                     /locus_tag="PLP50_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51034.1"
                     /translation="MDAAAYSAFTRELARRLGAEPRVVGLVALGSMSGDGPPADDFSD
                     HDFFVVTRSGVQEAFRSDLGWLPGAERAVLVHRETEHGLKVVLDDGHLLELAVFDLEE
                     LRVARANRYLVLLDRGGVAERMAAVAAKPAEAPTDRWLSGQLLGNLLVGGGRAARGEL
                     LSARLFVAGHATGHLLRLLSRHVPPEAPVPLDDLDPFRRFERAFPILGAELNAALSLP
                     PLPGALALLDLASRELGGRVAAWPAEAAETVRRRLSGMRDGGDAG"
     gene            34767..36137
                     /locus_tag="PLP50_05465"
     CDS             34767..36137
                     /locus_tag="PLP50_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPA51035.1"
                     /translation="MLFKTIIEPFRIKSVEAVKFTTREEREKALVEAGYNVFLVPAED
                     VLIDLLTDSGTGAMSSAQWGALMQGDESYAGSRSFFRFRDVVQDLTGFKHVIPTHQGR
                     AAERILFHTVLKPGMVVPSNTHFDTTRANIEVEQAEARDLVIAEGKVPSLIHPFKGNV
                     DLAKLEKTLAEDGDRVPLVMVTVTNNSGGGQPVSLENLRGVRALCDTYGKPFFLDACR
                     FAENAWFIKQREPGQSDRTPKAIAQEMFSLADGCTMSAKKDGLANIGGFLAMNDDELA
                     ASCRSLLILTEGFPTYGGLAGYDLEAIARGLEEVVEEPYLRYRIRSTAYLADKLTAGG
                     VPIIQPPGGHAVYLDARAMLPHVTPLQYPGIAIANALYLEAGIRGVEIGTVMFGLHPD
                     GTEAPGPMDLVRLAIPRRVYTQSHIDLVAEGVLEVFRKREELRGYRITEAPKVLRHFS
                     ARFAPL"
     gene            complement(36210..37673)
                     /locus_tag="PLP50_05470"
     CDS             complement(36210..37673)
                     /locus_tag="PLP50_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51036.1"
                     /translation="MTDTPTDPHPLSLVVVGAGPAGLTAAWENARHGGTATVLERDDL
                     VGGIARTVERRGYRFDIGGHRFFTKVREVEELWHEMLADDFLRVPRQSRILYRRKLFS
                     YPLKPFEAFFGLGPIESFRCGLSFLAARLAPRSPEEDFETWVSNRFGRRLFSIFFKTY
                     TEKVWGVPCTQIRADWAAQRIRGLSLWKAVWHAVKPDRSVKTLIEEFDYPRLGPGQMW
                     ERFTARLEERGSRVLLGRSVARIAWQEGRGVTAVETDGSAGRETFTGDAYVSSMALSD
                     AVLALDPPPPPDVLEAARALRYRDYILVALMFEGERFFPDNWVYIHSPEVRIGRVQNF
                     GNWSAAMVPRPGVTCLGLEYFCFDTDPVWSAPDDELLETGRSEITKTGLVPEGARVLD
                     GAVVRMPKTYPMYDPGYSEKVDVVRRFFAERLPNLQQVGRNGMHRYNNQDHAMVTGLY
                     AVRNLYGASNDLWAVNVDDEYHEETRTLRPGEEGSRP"
     gene            complement(37769..39541)
                     /locus_tag="PLP50_05475"
     CDS             complement(37769..39541)
                     /locus_tag="PLP50_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPA51037.1"
                     /translation="MARHGADRTVPRAPQASGGELVAEVLGRHGVRFLFTLCGGHISP
                     ILAAAKARGVRVVDTRHEATAVFAADAVARLTGVPGVAAVTAGPGVTNALTALQNARL
                     AQTPLVLLGGATATVLRGRGALQDVDQIALVAPVVKRAYRVSKLRDLVPALEEAFAVA
                     RDGVPGPVFVECPVDLLYPERVVRGWYASKAGTPKGLVAKATRWYLDRHLDALFTGVD
                     GVDARGPASAPPPPPEAGSVGTAALLLGRSERPVALLGSGALLDPAGAGALARAVAEL
                     GLPVYLSGMARGLLGPASLLQCRHRRTDTLREADLVLLAGVPCDFRLDYGRQIRPDAV
                     VISANRDSAARALNRRPTLDVGGDPGLFLRALADAVPGGFPRPEWLARLRERDATRGA
                     EISAAPDGARGALDPVALCRSLEEALPEDGLVVADGGDFVATASYVLRPRAPLSWLDP
                     GVFGTLGVGAGFALGAALCRPGAPVLLLWGDGSAGYGLVEVDTFVRHGLGVVAVIGND
                     AAWTQIAREQVEVLGDDVGTVLRSTDYHAAARGLGGEGLLLDDPTRRGPVLAEAFRLA
                     RTGRPVVVDARLGKTAFRKGSISI"
     gene            complement(39563..41752)
                     /locus_tag="PLP50_05480"
     CDS             complement(39563..41752)
                     /locus_tag="PLP50_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPA51038.1"
                     /translation="MKAEGASPDRELLAGQVERVTFHSEETGFAVLRVRVRGRREAVT
                     VVGHAAAISAGEELRASGSWGNDPKHGPQFRAESLTTVAPTSAEGIERFLASGLVRGV
                     GPHFARRLVAAFGTGVFEVIETAPARLLEVPGVGPVRAERIVASWAEQKAVREIMLFL
                     HAHGVGTSRAVRIYRTYGADAIAVVSEDPYRLARDVRGIGFVTADALARRLGIPEDAM
                     IRVRAGLSHVLADALDEGHCGLPRETLIPRAAELLGRPETQVAEALELELAEKRLVPE
                     EAGGRPCVFLPALHAAERAVAQRLATLASGTTPWRRVKPEEAVAWVEERTGITLSASQ
                     RAAVATFLRSKVLVLTGGPGVGKTTLVDALLRIVLDASLRIALAAPTGRAAKRLSEST
                     RSEAKTIHRLLEVDPSTGRFRRGRDNPLDADLVVVDEVSMVDVLLARALLEAVPNGAA
                     LLLVGDADQLPSVGPGQVLRDLLSSDAVPSVRLTEVFRQAAESRIVVGAHRVREGHLP
                     DLTNPAGTDLFFFDAKEPEEAARRVVEVVAERIPRRFGLDPRRDVQVLVPVRRGPLGA
                     RALNEALGKALNPGGAPRVSRFGMELAPGDRVMQVENDYDREVYNGDLGLVTSVDPGE
                     GELRVAFDGREVTYGFDELDLLQLAWATTIHKSQGSEYPAVVIPLAMTHYAMLERNLL
                     YTAMTRGRKLVVLVGDRRAIAVAARRSSAGSRVTRLAELLRAARPGC"
     gene            41786..42823
                     /locus_tag="PLP50_05485"
     CDS             41786..42823
                     /locus_tag="PLP50_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51039.1"
                     /translation="MRNVRAVPALLAALLVAAAAPAQEPSRRGSIAAKAGVSKVLVDA
                     HVLGPGGRTLLGLTPSDFLLRVGGEEVPVEGVEFVPAERSELPPRPAGQAVEPGTPEF
                     PDGRLIVLFFQADFGRYRVKGQMRMANEMVRFLDRLLPTDRVAVVSYDSHLKLRLDFT
                     DDRKAIHRALFDTLKIGGAPDPPPASPFPSLAAHFDVEEARAAANVERGLYVVAKALE
                     KIPGGKSMFFLGWGLHVNRAPADWKWHALAVNALQVARVNVFTLDVSDADWHTLEGTL
                     RKLADLTGGTYAKTHVFSSQALDLAFRAIEGRYVVVFDRPDRPPGRHAIDLTLRMKKG
                     RVLARPFFDDP"
     gene            complement(42860..>43323)
                     /locus_tag="PLP50_05490"
     CDS             complement(42860..>43323)
                     /locus_tag="PLP50_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51040.1"
                     /translation="CVIIGVSMGINLWPLWSAGGLRAPVADLLPIASIFIILTVGLIA
                     FGELYTSPRMYEYIGALAPKGQEGLFLGYANLPLAIGSLTGGPVGAWIFNDVMAKGAT
                     TRADGLLELNRAAATRGWLLLMAIGFLSAVAMWAFNRWVEKQEAKEAPAAV"
BASE COUNT         6255 a        15289 c        15980 g         5799 t
ORIGIN      
        1 gccgcgagcc cctgcgtgtt cggcgggagc tcccagacgt cgtagccgcg gtagcccgtc
       61 gacatcggcg cgtcccacgt cgagcggtgg cggacgaagt cctccctttc gaagaagccg
      121 ccgttcgcct tcgagaaggc gacgatcgcg tcggcgaact cgccttccca gcctgctctc
      181 ttcttcgtcg cgaggagccg gagcgtcttt gcgagcgccg ggttccggaa gcgctcgccc
      241 tccttcgggg cgcggccgcc ggggaggaag acctccgcga agccgggctt gtccttgaag
      301 cggttcaccg acccggccca gttgcccgcg atgacgggcg agacggggaa gccctcctcc
      361 gcgtagcgga tcgccggggc gaggacctcg gcgagcggga gcttcccgta ccgcgcgtgg
      421 agctcgagcc agccgtcgcc gcagcccggg acgctccagg cgtaggggct gaagagcggg
      481 atcgtcccgt cggggagcgg cgggaccttg tcggcggtca gcgcgagcgg cgcgcgcccc
      541 gaggcgttca ggccgaccac cttcttctgc gcggggtccc agaggatcgc gaagaggtcg
      601 cccccgaggc cgttcgccgt cggctccatc aggccgaggc aggcgttcgt ggcgatcgcc
      661 gcgtcgacgg cgctcccccc cttgcggagc gtctcgacgc cggcctgggt gcagagcggc
      721 tgcgcgctcg cgaccatgcc gtgctccgcc cagaccggcg agcgggtcgc cgcgccgcgc
      781 cccttcggtg gccactcggc gaggagagga aaggcgatgg cgagggcgag gagggcggcg
      841 agggaggcgc gcatgccggg atcgtacgcg gcgaaggagg actgcggtca ccggcccgtc
      901 cgcgttctag actctcgcgg atgttcgagg cggcgagtag ctcgaggttc cagggggggc
      961 tcctcccgcg gcggacgttc ctcgcgctcc tcgcgttcgc gtcgctcctc gggccgcttc
     1021 tggtcttccg cgagaccgtc cgcgacccgc tcgagcggat cccctgcggc cgccccgtcc
     1081 cgaacgagcg gctcggagac ccggtcatcg tcctgtcggt gatgggccgg cagtgggcgc
     1141 gatgggactc gggcgacttc tcgcggcgcg acgaccgcgt cttcgcgccg tatcccgata
     1201 cctggcctct cggagaaccg ttcctcctgc cgtcggcgat cggttacccc tggtcccgcc
     1261 tcgcggactc ggacgcgctc ggctacaacg tgccgtactt cctcgcgacc gcggtcgcct
     1321 gcttcgcggc aggactcctc tgtgccgatc tcgtcggacc gggttgggcc gcgctcatcg
     1381 gagcgcttct tcttgcctgg agcccgggtc gactcaacaa cttcggcgtc ctcggaacga
     1441 tctgggcggg cttcgtctcg ctcgtcctgc tcttcgcgct ccggtacctt cggagcgggc
     1501 gtgtccgcga cgcggccgcc tgcgccgccg ccctcctcgt cgtcgggctg gggagcccgt
     1561 atccgctcgt catgggaacg gtctccgtcg ccgtcgtggc cgccgcctcg gcgaggagct
     1621 tccgacgtcc cgtactcctg ggggtcggcg ggagcgccgc gatcgtgctc ctcgcggtcg
     1681 catacgcccc ctacctccac ctcgcccgcg acttcgacgc ccccgtcggc ctgcggtcga
     1741 tcgaggttca cgcggccgac ctcctctcga ttctccacac gggcgtcttc gccgggccgc
     1801 tccgggacct tctcgacggc ctcgtcccgg tcttccccga gggagcctcg gcgctcttcc
     1861 cgacgctcac cgcgctcgcc gccttcggcg cgggcgcctt cctgcttcga cggcgcggcc
     1921 cgtggaggcg gcgggacgcg cggcacgccg cgcgatcgct cgccccctgg ctcctcctcg
     1981 ccgggatctc cttcgtcttc gctctcgggc cgacgatccg tttcgcgggg cgcgcgctcg
     2041 gccccgggcc gtacgccctc ctcgcccagc tgcccggctt ctccggcatg cgaggaatcc
     2101 accggtggga ccagtggttc ggcctcgggg tgatcgtcac cgcgaccctc gtcctcggtc
     2161 ggctccttcg cgcgcttccc gcgcgccggg ggcggctcct cctcgtcggc gtcgcccctc
     2221 tcctcgtcct cgacctctgg acgaggccgg tgccggcgca accggtcccc gcgccttcgc
     2281 cgttcgacgc ggagcttcga gcgctcccac gcgacgcgat cgtggcggtc tggcccttcc
     2341 gacgcggcac gtcggaacgc tcgtgggccg agcagctctc acaccgccgc cgtgtcctga
     2401 acggcttcca gacctatcct ccgccgatcc acttctggct cgactccttc ctctcggaca
     2461 agccgttcga ggcggcgttc gccgcgtact ccgagctcgg cgtcgccgcg atcgaggtcg
     2521 acctcgaggc ggccccgcgc ggttcgggcg agcgcctcgg ggcgctcctc gcgtcgggag
     2581 ccctccccgg ggtccgagcc gcgatgggga gccggtcgcg gctcctcctc ctcctcgagc
     2641 cgaaggcgcc gatcctcgtc gatccccgaa cgatcgaggg gctgagcttc gaaggagact
     2701 cggccgtcgt cgcgcgggcc gaggggcgtc tcctcttccg tctcgggagc gggcggctcg
     2761 cggtcgtcgt cgagtcgccg gcgggccccg tctccgacgt cctccggatc ccggtcgtcg
     2821 gcgcgggcga gctgggcacg cgcctttcac gttcgctccc gcccggtgcc gtcgtccggg
     2881 ccgccgccga cggccgcgag atcggacgga ccggtccgcc tccttcccgc ccgccgagcg
     2941 gataatcccc gagcccgtgc gcgacctcga gcctcgacag aggcggatcg tcctcttcgc
     3001 cctcggggcg gccctcgtgg tctccctggc gtggacgacc tggctcgctc acctcggggt
     3061 ggagcgcggc gcgctcctcc gcgcccgact ctggctcgcg ctcctggtct tcggcgtcca
     3121 ctacgtgggg gcggtcgtcg tcctgctcaa gcgcccgtcc gaccccgcga accaggccct
     3181 cttcccgttc ctgaccctcc tcggcgtcct gaacgcgggg cgcatcctgg gagtcgtgac
     3241 gggcggcgtc acgttccagg accggatcgc gtgggccggc tcgaccgccg tgcacttcgt
     3301 cgcgatcgcc tggatcctcg tgacgatcgg cggcttcttc ccggtgactc gcctgcacag
     3361 gccggggcgg gcctacttcg tcggcacgct cgtcgcgatg gcgcccctgg ccttctacct
     3421 caggcgatgg tggctcgacg gcggggcggc ggccccgggc ggcgcgtgga ggaccggagt
     3481 ggcgatcgtg gcgctcgcgg tcgacctctt cgtcttcgtc tacttcctct ccctggcctg
     3541 ggtcgcccgg aaggaggacg tgcgacggcg ggcacgcgtc gtcctcgtgg ccgtccttct
     3601 ctacctcggc ctctacatct tcctgcgcga cgtcccggcg gtcgccggtc ggagcgcgct
     3661 tctgccggtg ccggtcctct ccacgctttc ggtcctcctg ccgctcgccc tcatcgtcgc
     3721 ggcgacgcgc ttccgcctgt tcgaggtcga tcgcctcatc cgccggagcg cctcctacgc
     3781 catcgcctcg ggcgccctcg tcgtcctcct cctggccggg gtcccggcgc tcacggcgct
     3841 cgtggctctc gtctcgcccg ccctccaggg gacgctcacg acggccctcg tcgccgtgct
     3901 cctcttcctc gtcttcgacc cgctccgaaa gagggcgcag atcgcgctcg accggaagtt
     3961 ccggcagggc gcgtacgacc cggcgcagct cgtgaccgac gtcgtcgggg atctccggga
     4021 cgccggggag gacctggcgg gcgtcgtcct tcatcggatc gcgtccgcgc accggcccga
     4081 ccggcaggcc ttctgggtct tcgccctcga ggcgccggga gcggccgtgc ggcccgtgac
     4141 gacgggcgcc ctcgcggacg tccccgggct ggaccgcgca gccgtcctcc cgatgtcgga
     4201 ccctctcgag gcgcgcaact cctttcacgc cctgacggcc gacggcggag acccgcccta
     4261 cgccgaggga gagctcgcga acggccttcg ggccgccggg ttcgtcgcgg ctctgccgct
     4321 cttctcgggc gacggggagc cgctcggcct gatcgcgctc ggcgccaagc ggacgggaga
     4381 gacgtacgtc ccggaggagc agaggatcct cgggaccgtc gcggggcacg tcgccctcgc
     4441 cctcgagaga gcggccctga cgcgccaggt ggacgaggac cgtcgcgttc gcgagaaggt
     4501 actcgggcac cttcaggaag gcgggagcgg ggcgctctac gaatgcgagc tctgcggccg
     4561 ggtcgcggac gaggacgacc tcgtctgcac ggccgacggc cacgcggtga agatgacgca
     4621 ggccgtccct cggctcctcg ccgggcgcta ccggctcgac cggcggctcg gcgagggagg
     4681 gatggggacc gtctatgccg cgcgagacat ccggggcaat cgcgacgcgg ccgtgaaggt
     4741 gatccgggcc gagcacctcg ccgaccggag cgccgtcgcc cgcttccggc gggaggcccg
     4801 gctgacggcc cgcctcggac acccgaatgc cgtcgccgtc tacgactacg gcgagctccg
     4861 gcccggcggc gcgttcctcg taatggagct cctctcgggc ggctcgctac ggaacgagct
     4921 gatcctccgg aagccgatcc ccttcctcga gacggcctgg gcgctcgacc gcgcgctcga
     4981 cgtcctcgcg gcggcgcatg ccgcgggcct cgttcaccgc gacgtcaagc ccgacaacct
     5041 cttcgtcacg cgcgggccgg ggggggccac gaggctcaag ctcctcgact tcggcctggc
     5101 gcgggaaacg aaggcggaag gcggggccga gagcggcggg gcgatctcct cggcgagcat
     5161 cctgatgggg acgcccggct acatcgcgcc cgagatcgtc tcgggcggag aggcgacgcc
     5221 cgcctcggac cagtgggcga tcgcggcgac cggcgtggag ctcctgaccg gagtgcggct
     5281 caagttcgac cgacccgacc tgacgctcaa gtccctcgtc gaggaggtcg tctcggtcgt
     5341 gacgaccgtc gcctcggggg tcccggtcac ctacgcccgc tccctcgcca aggggatggc
     5401 gatggacccg gaagaccgtt gggtcagcgt cctcgcgctc cgccgcgccc tcgaccggag
     5461 caccggcggg cgaatcccgg gtgacggcga cgtggagttc gacgtcctcc gggaggagac
     5521 gtagcggccg ccggggccga gacgccggcg gggctcaggc accatcggcg atcgaacggt
     5581 cgtcgaacgg cgacccgctc gcgcgacctc tccggctttc cacgagggcg gcccggagct
     5641 cctcttcgct gatcagctcg aggtgtcccc agcgatcccg gtagatcgtc cccctccgga
     5701 cctgctcccg ggcccagtga gcccccgagt ccattccggc gaggtctccc acgacgagga
     5761 cctcggtcac ggcgttcagg aacgggtgga tcgacccccc acgtgcctcg atctctcgct
     5821 cgagcgcgcg aagcacctcc gtcggctggt tgctggcgac gacgaaagac cgcccggcga
     5881 accggatggg gggccggacg tcctcctcag gtgccgtccc gttcgcgctt ctcatagacc
     5941 tccccctttc ggggagccgg tccgaggacg gaccccggtc tcggcatccc tctactcctg
     6001 atgagggatt caggggggtc cgagtgcccc tctccccgaa gtttttttca cttttttttg
     6061 gggagggggg gcagttacgg gggtcgaatc ccctcatacg tagtgaatgg ggtctcggcc
     6121 cctgggcaaa ggccggtcga aagggccgga atggaggatt catggatgtc ctggtgttga
     6181 tcctgcttct cgtcaccggc ctcggggcgc cgtcgacgga cggcacttcg gagacctcct
     6241 cggtcacgac atcgtccacg acgacggaac cgacgctcag cgatgggggg gacggcgtgc
     6301 ggaagcagcc gatcggttga ccggagcttc tgtcaggccc gtttcgccgc ctcgaaccgg
     6361 tctcgaccgg agaggtagtg gcggagccgt ctgagccggc cggccatctc gtggtccccg
     6421 gcgtcgaggc ggtccaggag aacggtcgcg tccggatcgt cccggacctc gggcggagcg
     6481 tctccacgga tcgcttcgac cagcgcccgg acctcgccgt aattcccgag ggccgcctgc
     6541 gcctcggcca gggcgagcct cgcgtagtag gcgtcgaccg agagcccctc ccgggcgcag
     6601 aaagcgacga gcttcttccc gcggacgcgg acatcctcga acgtcccctc ctcgagaccg
     6661 aggttcagca ggtttacccg gaccgtcgtc gcggccgccg agaggccgtg ccggaccgcg
     6721 agctcgaggg cctgtccgaa ggccgatctc gcctcggaga gacgcccgag gttcatgagg
     6781 atcccggcgc ggttctgctg aatcgcctcc cagggaccgg ggtccacgtc cgggtcgagg
     6841 cgctcgaggg cggcgtcgaa ggcgtgcagg gcctcgcgga accgggcctc gctgaaatgg
     6901 acgagtccga tcgctccctc ggcccggccg acccaggggt cctgcccgtc cgcggcgaac
     6961 gcctcgcgcg ccgcgaggag acggctcaac gcggcctcgg atcgacccgc cccccagagc
     7021 gccgagccgg cgtagtagtt ggcacgcgcg cgcagcaggg cgggggcgtc ggccgcctcg
     7081 atcgccgaag aggccgcgag tgcccgctcg cacgcctcgt cgatccgccc cacgtagacg
     7141 aaggactgac tctccaggag gccgatctcg gccgcgagaa gcgcctggtc ccctccaacg
     7201 tctccgtgca gacgctggcg gatcgatcgg gcgaacgaga gcgccaccga tggtcgccgg
     7261 gacgtgagcc tcgaggcgat ctggagcgcg tgcagcgcga cgtccgggaa gtcggggcga
     7321 gcggaggcct cgtcgagcca ggccgggaga gcgtcgtcgt cgcgttccgc cgcgcgaacg
     7381 agctcctccg cccgggcgag cgtccgggag aggcgggaga gcgccgcagg cacgggaacg
     7441 cgggggcccg attccgcttt ggtcgcgagc gcggcgagat cggccaggtg ctcgcggccc
     7501 gccgcgagcc cctcgatcct gtcccggcag gtcgggcaac tcgcggcgtg cgggtcgggc
     7561 gccagtcccg gcgtcatcgc ccgctccagc agctccagct ctccgagatg acgatcagcc
     7621 gagggcatgg gcgagcgtcc tcaggcactt ccggtagcgc ttgtagacgg tgcgggcggc
     7681 ggacgaggaa tcggagccgg cgcgacttct gccgccgagc aggcagctcc gcatcatcga
     7741 gcgacagacg ggagagagga gcgagaacgc ctgccggaac cggcaggcga cctcgatccg
     7801 ctcgcaggag tcgtcggccg catcgaaccc gaccggcgga gcgacctccg tccgggcgag
     7861 ctggctccgt cgccgcagga ttccgaggca ttcgcgtagg aagaccgagg tgaggtaggc
     7921 ctcgggggac cggacggacg gagcggccag gacgatggcc gtcgccgcca tctggagcgc
     7981 ctcctccgcg tcgtcccggc tgagcccgaa gcggcgcgcg ccgaggacga tgaggcccgg
     8041 tcccagtcgg gagtagaggt cttcccagtc ccagtagctc ggctccaggc cggccggtcg
     8101 ctccgcctcc ttcggcgtag cgaatcgcga agccgcccag gccacgttgt cgagcggtgc
     8161 ggtctctccc ggcacggggc gattatccct gccccggccc tccgaggaaa accctcacgt
     8221 ttcggggcac gacacggagc cggtcgcccg gggagggcgc gagctcggcg aagcgctccc
     8281 tcgagatctc gaccttgagg atcgtcccgt cgtcgggggc gagctcgagg cggacgacgg
     8341 cgccggccgc agcgacgtgt cggacggtgg cggggaagcc ctccacccct tcgaggtcgc
     8401 gcgagaggtc gatctcccag ggacgagcgt agcctgaggc cgggagcgcg ccgtcgtggg
     8461 gggcgtcggg gtacgcgacg gagagagggc cgaggtgggc ccggccgttc tccacccggc
     8521 cgtggaagat gttcacggag ccgaggaagt cgaggacgaa cgggtccgcg gggtgctcga
     8581 agaggtcggc cggtgtcccg tcctggacga cgcgccctcg gtcgaggagg acgacgcggt
     8641 cggccacctc gagcgcctcc tcctggtcgt gggtgacgaa gaggctcgtg aggcggatct
     8701 cgtcgtggag gcggcggagc cagcggcgca gctcctggcg gacccgcgcg tcgagcgaac
     8761 cgaacggctc gtcgaggagg aggaccttcg gctcgacggc gagggcgcgg gcgagcgcga
     8821 cacgctggcg ctggccgccg gagagctcgg acgggagacg gtcgccgagc cagtcgagct
     8881 ggacgagctt caggagctcg ccgacgcgcc gcttcacgtc ggcctccgaa gggcgctcgc
     8941 cgcgcggctt gacgcggagg ccgaacgcca cgttctcgaa gacggtcatc cggcggaaga
     9001 gcgcgtagtg ctggaagacg aagccgacgc gccgctcgcg ggcgtcgcgg cgcgtggcgt
     9061 cctccccgtg gaagaggacg gtgccgcggt cgggctcctc gaggcccgcg atgatccgga
     9121 ggaggctcgt cttgccgccg ccggaagggc cgaggagggc gacgagctcg ccgtcgccga
     9181 cgcggaggga gacgtcgtcg agcgcgagga agctcccgaa cgacttcgag acggaccgaa
     9241 cctcgatgct cacggggcct cctccctcgc gcggcgcgag acgatcgcct tcgccaccat
     9301 cgtggcgagg gcgacgaacg tgaggagcga ggcggccgcg aacgccccct ggaaggcgta
     9361 ctcgttgtag agggtctcga cgtggagcgg gagcgtgttc gtctcgccgc ggacgtgccc
     9421 cgagacgacg gagactgccc cgaactcgcc gacggcgcgg gcggtgcaga ggacgatgcc
     9481 gtagaggagc gcccacttca cgttcgggag cgtcacgcgg aagaacgtcg tgaagaagcc
     9541 ggcgccgagg acccgggccg cctcctcctc gtcggtcccc tgcgactgca tgagcgggac
     9601 gacctcgcgc gcgacgaacg ggaacgtgac gaagacggtc gccaggacga tgcccgggag
     9661 ggcgaagacg atctcgagcc cgcggtcggc gagccagggg ccgaagaagc cggtgcgtcc
     9721 gaagaggagg acgaagacga ggccagcgac gacgggcgag acggcgaacg gcaggtcgac
     9781 gagcgtgagg acgaaactgc gccccgggaa gcggaacttc ccgagcgccc agcccgcggc
     9841 gaggccgaag aaggcgttca cggggacggc gatcaccgcg acgaggagcg tcagcttcag
     9901 cgcgtggcgg gcgtcgggct cggtgacggc ggcgagccag gcgcgcgcgc catcccggaa
     9961 ggcctccacg aagaccgccg cgagcgggac gaagaggaag agggcgaacg ccgcgagggc
    10021 gaggccgatc aggaggagcc gcacggcgcg cggctcgcgg ctccgcgggg cggtcaccgg
    10081 acggccctcg ccgcccacgc ctcgaggcgg ttcaggacga ggaggaggag gaaagaggcg
    10141 acgaggagga gcgtcgcgag cgcggtcgcc ccggccacgt cgtactgctc gagcttcgtc
    10201 atgacgagga gcggcgcgat ctcggtcctc atcggcatgt tcccggagat gaagacgacc
    10261 gagccgtatt cgccgaggcc ccgcgcgaac gcgagcgtcg agccggtgag gagcgcgggg
    10321 aggagctccg ggaggaggac gcggcggaag gtctcgagac gcgtcgcgcc gaggctcgcc
    10381 gcggcctcct cgacctccgg gtcgaggctc gcgaggaccg gctggagcgt cctcacggtg
    10441 aaggggaggc cgaggaagac gagcgcgacg ccgatgccga gcggggtgaa cgcgaccggg
    10501 acgccaagtg cggcgagagg ccttccgagc cagccggtcg gggcgtagag cgtcgtgagc
    10561 gtgatcccgg cgacggcggt cgggagcgcg aacgggaggt cgacgagggt gtcgaggagg
    10621 cgccgtcccg ggaagcgata gcggaccagg acccagccga ggaggaggcc gaggagcgcc
    10681 gagacggccg ccgccccgag cgagacgagg accgagagcc tgaccgcggc gacgacgcgc
    10741 ggcgtcgaga cggcggcgag gaactcgcgc ggcgttccgc ggagcgtcgt cgccaggagc
    10801 gcggcgagcg gcgcgaggac gacgagggag aggaagagga gcgtcacccc gagcgccggg
    10861 ccgaagccgg ggaggagcga cggggagacg cggcggcggc tcacttcgtc gtgaagaggg
    10921 agtcgaagac gccgccgtcg tcgaagtgcc gcttctgcgc ggcgcgccag ccgccgaaga
    10981 gggcgtcgat ggtgaaggtc gcgacggacg ggaaggtgcg cccggccttc gcggcggcct
    11041 cggcgaggcg cggccggtag tggtggcggg cggcgatcgc ctgcgcctcg ggcgtgtaga
    11101 ggaagcggag gtacgcctcg gcgaccgcgc gggtcccctt cctgtccgcg acggcgtcga
    11161 cgacggccac cgggggctcg gcgaggatcg acgtcttcgg gacgacgatc tcgacgcgcc
    11221 cggccccggt ctcggccagc gcgaggtgcg cctcgttctc ccaggcgacg aggacgtcgc
    11281 cgagcccccg ctggacgaac gtcgtcgtcg agccgcgcgc gccggtgtcg aggacggcga
    11341 cgttccggaa gagcccgccg acgagggcct tcgcggccgc gtcgctcccg ccggtcgtga
    11401 ggacggagcc ccatgccgcg aggtagcccc agcgggccgc gccggacgtc ttcgggttcg
    11461 ggcagacgac ggagacgccc gggcgcgcga ggtcgctcca gtcgcggatc cgcttcgggt
    11521 tcccggacct cacgaggaag acgatcgtcg acgtataggg ggaggcgttg tgggggaggc
    11581 gggcctgcca gccgcgcgcg acgaggccgc ggtcggcgag ggcgtcgacg tcgtacgcga
    11641 gggcgagcgt gacgacgtcg gcccggaggc cgtcgatcac ggagcgggcc tgcttccccg
    11701 agccgccgtg cgacatcttc acggtgacgg cgcggccggt cctcgcgagg aagtccttcg
    11761 cgaaggcggc gttcagctcg cggtagagct cccgcgtcgg gtcgtaggag acgttcagga
    11821 tctcgaccgg ctccgcggcg cgcgcggcgg cgccggcgag agcgtggatc gcgaggagcg
    11881 aggcggcgga ggccgcgaac ccgaggaggg agcggacggg gcgtcgggaa tcggtcatca
    11941 cgtctccggg actccgcccc tcctgcggcg cgcgacgagg atcttccggt cgcgctcgag
    12001 gaggtcggcg agcgtcgtgt ggtcgaggat cgccgcggcg gcgttcctca cgtcgaggaa
    12061 gaggtcgaag agcccggcgt gccgcggctc ggacgcgacg agccgggtga gctcggtggc
    12121 gtcgccgaac ggcgcgagcg gtccgtcgag gaggcggacg acgtcgccga cgacgatctc
    12181 ggccggcggg cggcgaaggc gatggccccc ttcgcgtccc cggcggctct cgacgacgcg
    12241 ggcgttcttg aggacgacga ggatcgactc gaggaacttc tccgggatct cctcgcgctc
    12301 ggcgatctcg cgcgtcgcga cgatcccttc ctcccaacgt cccgcgaggt gaaggagcgc
    12361 cttgagggcg tagaggccgc gctgggagac tttcaaagta ttcctacagg attagtagga
    12421 tatttgcggt cccgggcctt cgtcaaggac cggaagcctc ctcggcttct cccggggcgg
    12481 cggggtgcgt tacgtcccgt ggtggcggcc gaacgacgga gacggaacgc cccggggcgg
    12541 gggccccggg gcggctcagg aaggggcaga ggccgggcga cgggcgaccg gtcagcggtt
    12601 cggctcggga cagtagcggt cgaacccgcg ggatcgctcg cggcgggcga gccggtcccc
    12661 gtcgggcggg aggaggccga gccggacgag ctgcggccgg aactcgtagc ggatccggag
    12721 gcgcgcggcg ggggagcgct cgaggtcgag gtcgacccgg gtcacctcgt gccgcgtccg
    12781 gtcccccatc ccggtcgcag cgtattcgtc ggagagggac ggcgcggcgg ccatcttctc
    12841 ggccggagcg ccggcctcgc ctttggcgcg ggacgcctcg gagcggggcg cggcgtcttt
    12901 cggggcgcag gccgtcggcg agaccgggag ggcgggccgg accttctccc ggaagaagac
    12961 cgcctcgatg acgccgaggt cctccacttt cccgagcgcg gcaccgtagg agtcgcgctc
    13021 gcccgtgaag acgaatcgac gggcatggga cgagctgacc tgccaaccgt cgaggacgac
    13081 ggactggtac ggttccagga tccacttcac ggcggaggcg gcatcccggc gggaggcgtc
    13141 gatcgtattg agcccgtcga cggccagggc gaccccgacc cggacgccga gcgggttcgt
    13201 gacgcgaagg gcgtacgggt gccccttcag cgcctcgacg tagacggagc cccctttccg
    13261 gagctcgggg cgctcctctc cgtccacgag gacggatagc tcgaagccgg cgcgcgtcgc
    13321 tgcggcggca gggaaggaag cggcgaaggc gagggcgagc gcggcggcgg tacgggttct
    13381 catcggcgtc ctccgcccga tgagaccttc cgacggcagg atggttcccg gcggcggccg
    13441 cgaaaatgga accgagtcgc gacgggagcc ggcgtgagga acgaggctcc ccggctcgaa
    13501 agaccgccct ccgggcgtta tgatgccctg cttgcctcgc gggcgccccg gaagcggttt
    13561 tcggcgggcg ccgggaggcg gcatcggagt ccgacatcag aagcgaaacg aaacgccggt
    13621 gcgccgctcg gggggatggg ggggagacgt tctcccgcct ccggccgggc gcggccggca
    13681 cgggaagaag ggcatgaacg tactcgtcct caactgcgga tcctcgtccc tcaagttcgc
    13741 ggtcatcgac cccgacacgt ccgtcgagca cgtctccggc atcgcgcagc ggctcggctc
    13801 gcccgaggcc tcgctcgacg tgaagcggaa cggcacgaag agcacgcggc cgatcccggg
    13861 cgccctccac gacgaagcgc tccacggcgt cgtcgacgcc ctgaaggagc tcggtctcgc
    13921 cgacggcctc ctcgccgtcg ggcaccgcgt cgtccacggc ggatccaagt tcgccggctc
    13981 gatggcgatc acgcccgagg tcgtcgcgaa gatggaggag tgcatcccgc tcgggccgct
    14041 gcacaacccg ccgaacctcg tcgggatccg gatcgcgcag agcctcttcc cggacctcct
    14101 gcaggtcggc gtcttcgaca cggcctttca ccagacgatg ccgcccgtcg cctacaccta
    14161 cccggtgccg ttcgagtggc tcgagaagca ctccgtccgg cgctacggct tccacgggac
    14221 gagtcaccgc tacgtggcgc gcgaggcgct tcgcctcgcg gggctcccgg cggacgacca
    14281 cgcgatcgtg acggcccacc tcggcaacgg ctgctccgcg gcggcgatcc ggaacgggaa
    14341 gagcgtcgac acgacgatgg ggctgacccc gctcgagggg ctgatgatgg ggacccgctg
    14401 cggctcgatc gacccggcga tcatcggcca cgttgccgac gcgacgggga agagcgcgaa
    14461 ggagatcctc gactccctga acaagaagtc ggggctcctc ggcgtctcgg gcctctcgaa
    14521 cgacatgcgg acggtcgagg aggccgcctc gaacgggaac gaccgcgcga agctcgcgct
    14581 cgacatcttc tgctacgtcc tcgccaagac gatcgccgcg ctcgtcgtcc cgctcggacg
    14641 cctcgacgcg ctcgtcttca cgggcgggat cggcgagaac tccacgacgg tccgcgcgat
    14701 gacgctcggc cgcctcggct tcctcggcct cgcgctcgac gaggcggcga acgcgaagca
    14761 cggccgcgcg cagcacggcc ggatcacgac cggcacgaag ccgcaggccc tcgtcgtccc
    14821 gacgaacgaa gagctgatga tcgccgagga cgccgcgcag atcgcccgcg gcgcctgaga
    14881 cggaaggaag ggaagcccag atgcgcacca ccgtcctcgt cgcccccgcc ggccgcggcg
    14941 tcggcctgac gaccgcctgc ctcggcatcg tccgcgccct cgaccgcgag gggatccggg
    15001 tcgcgttcgc caagccggtc gcgaaccgcg agcccgacca caccattccg ctcctctccc
    15061 tcggcgccca cttcgcgatc ccgccggccg tgccgcgcgc ggaggcggaa ggtctcctcg
    15121 ccgacggcga cgaccagacc ctcatggagc gcgtcgtcgc catcgccaac gaggcgggcg
    15181 aggacgtcga cgtcctcatc gtccagggaa tgcaccccga gatggggatg gtccactcgg
    15241 cgcgcgtgaa cgcgctcatg ctcaaggcgc tcgacgccga gctcgtcctc gtggcggccc
    15301 cgtccgacca gaccgcggcc gaggtcgcgg agacggtcgc gatcgccgcg cgcggctacg
    15361 gggcgttctc ggagggacgg agcgtcggct gcatcctcaa ccgcgtctgc gaccgccggg
    15421 ggatgccggc cgagccggag gtcgactcct tcggcccgag cgccggcggc tgcggcgcgt
    15481 gcgcgccggc gggcctctcg gagacgtgca tgaaggacca ccatgcggcg ctcgaggcgg
    15541 aagggatccg cccgatcgcg atcgtcccgt gcaactccga cctgatgtcc ccccgtgtga
    15601 aggacatcgg cgacgccctc cgtgcccagt tcctctacac cggagaggcg gcgacccgcc
    15661 gcgtgcggga cgtcgcgatc tgcgcgatga cggtcccgaa cgcgatgaag gcgatgaagc
    15721 ccgggacgct cgtcctgaca ccgggcgacc ggagcgacat actcctcgcc tcggcgatga
    15781 cggtccagag cgggacgccg ctcgccggcg tcgtcctcac gggcgggctc ggccccgacc
    15841 gcggcgtcct cgacctctgc cggaaggtct tcgacgcggg cctgcctctc ctgcaggtga
    15901 aggcctcgag ctaccgggcg gcggcgcagg tcgcctcgat gaacccgacg atcccgatcg
    15961 acgacgcgga ccgcgtcgag cggacgatga gcttcgtggc cgaccacgtc gacctcgcct
    16021 gggctcgcgc cttcgcgaag ccggcgcgcg tcccgcgcct ctcgcccccc gccttccgcc
    16081 accgcctcgt ggagacggcg aaggccgacg tgaagcggat cgtcctcccc gagggggccg
    16141 agccgcgcac cgtcgcggcc gcctccatcg tcgcctcccg cggcatcgcg aagtgcgtcc
    16201 tcctcggcaa tcccgacgag gtacgcgacg tcgccgggcg gcaggggctg acgcttcccg
    16261 actccgtcga gatcgtgaac cccgcttccg tcgcccaccg ctacatcgcg ccgctcgtcg
    16321 agcggcgcaa gtcgaagggg atgaccccgg agcgcgccga gctggagctc caggacgcga
    16381 tcatggtcgg gacgatgatg atggcgctcg acgaggccga cggcctcgtc tcgggcgccg
    16441 tccacacgac ggcgcacacg atccggccgg ccctgcagat catcaagacg gtcccgggct
    16501 gcaacctcgt gtcgagcatc ttcttcatgt gcctgccgga ccaggtcctc gtcttcggcg
    16561 actgcgccgt gaacccgaac ccgacggcgg ccgagctggc cgacatcgcg attcagagcg
    16621 ccgactcggc cgtcgccttc ggcgtcccgg cgcgcgttgc catgctctcc tactcgaccg
    16681 ggacgagcgg cacgggcgag gacgtcgaga aggtgaagga agcgacgcgg atcgcgaagg
    16741 agctccgctc cgacctcgcc ctcgacggac cgctccagta cgacgccgcc gtgatgcccg
    16801 acgtcgcgag gctgaaggcg ccgaagtccg acgtcgccgg aaaggcgacg gtcctcatct
    16861 tccccgacct gaacaccggc aacgtgacct acaaggcggt ccagcgctcg gcgaacgtcg
    16921 tctcgatcgg gccgatgctc caggggctcg ccaagccggt gaacgacctc tcccgcggct
    16981 gcctcgtcga ggacatcgtc ttcacgatca acctcacggc catccaggcg cagcaggccg
    17041 cgcgcgcccg ggcgtcgaag ggctgacgcg tcttcccgga gggccgcgcg cgccgagagc
    17101 gcgtgcggcc ctccgcccct cccgcgagca cgcccggctg ccccgcccct tcacgattcc
    17161 ccccggaaga ggcaagatcc ggacatgaag aacgtcctcg cgcgccgaat cgccgccgtt
    17221 ctcgtcgcag ctgccttcgg ggtggcctgc gtcacgaatc ccgtcaccgg gaagtcgcag
    17281 ctctcgctcc tgggagagga ggaggagaag gccctcggcg cccaggcgta cggtccgctg
    17341 atccaggagt cgtacgggcc ggtcaacgac ccgggcctcc agaagttcct cgacggcgtc
    17401 gggcagggcc tcgcgaaggt ctcccaccgg cccgctctcg actacgagtt caccgtcgtc
    17461 aacgcgaact acgacaacgc gttcgccctg ccgggcggga agatctgcat cacgcgcggc
    17521 ctcctctccc ggatgacgac cgaggaccag ctcgcgggcg tcgtcgggca cgagatcggg
    17581 cacgtcaccg cgcggcacgg cgcccagcag tacacgaaac agatgctcat cggcggcctc
    17641 ctcgggatcg gcgggatgat tctcgaggcg gaggaggtcc gcggcgcgcc gctcatcatg
    17701 gcggccgccg gcatcggcgg gcagctcgtc ctcctccggt actcccggga ccacgagcgc
    17761 cagagcgacg agctcgggat ggagtacatg gcgcgcgccg gctacaaccc gaagggcttc
    17821 gtcgagtcga tggagatcct gaaggccggg cacgacacgg agccgtcgaa gctcgaggcg
    17881 atgttccaga gccacccgct gacctcggag cggatcgaga cggcccgccg gcgctccgcg
    17941 gagaagtacc cggagcagct cggccggccg gttcgcaccg agactttcgg ggcctcgacc
    18001 cgcgccctca aggcggaggc ccccgccttc aagctgatgg acgaagggga gaagctcctc
    18061 gcgaagaagg acgcccgcgc ggcggcggcg aagttcgacg aggcggcgcg cctcgcgccg
    18121 cggcaggcga tccttcccgc gctgaaggcg atcgccctcg tcgaggcgaa cgagcttcgg
    18181 tccgcccggg agtccgcgcg cgaggcggcc cgccgcgacc cggcgctctt ccacggccgg
    18241 ctcgcggggg ggctctcggc ctaccggctc tccgactggc gcgacgcgat ctccgagctc
    18301 gagacggcgg agaagtccgt cggcgcccag ctcgtgacga cctactacct cggccggagc
    18361 ctcgaggcgc tcggcgaccg gggccaggcg gccgagaaat acaagctcat cctccagagc
    18421 ggcgcgaccg gagagcaggc cgactacgcg aagaggcggc tcctggagtg gggagtcatc
    18481 tcgcagacgc cgcagcagca gccttacccc cagcagcagc ctcagcagcc ctacccgcgc
    18541 tgaggagcgg cgccgcgccc gcggcgcggc ccgcgcaccg gagcccccgg tcggcccgcg
    18601 ccctccgcgc gggccgtctc gtctctaccg tttcccgagg agagtcagga tgccggtcga
    18661 catcgccggg acgtaggtga tgaggaggac gcccgctccg aggatcagga ggtaggggac
    18721 cgcgtggcgg tagagcttcg ggagcgggat gccgaaacgc gaggaggaga ggaagaggtt
    18781 caggccgacg ggggggaaga ggaagccgag ctcgaggttc gcgaggaaga tgacgccgag
    18841 gtggatcggg tcgatcccga acgcggcgcc catcggagcg acgagcggcg cgaggatgac
    18901 gatcgccgag tagatctcga ggacgctccc caggacgagc agtatcccgt tcaggacgag
    18961 gaggaagacg agcggcgagg agacgtgcgc cttcacgtaa tcgaggagtg ctgcggggat
    19021 catcgcgtcg acgagccagc tcgtcagccc catggcgatc gacaggagga tcaggacggc
    19081 gcccatgagc gccccggcct tcacgaggac gccggggagc tgccggaacg ggtggatgtc
    19141 gcgcgtgacg aagcactcga cgatcgcggc gtaggcgcag gcggcggcgg aggcctcgac
    19201 catcgaggcg aagccgctcg cgaagaggac gacgacgagg acgggcagcg cgagctccca
    19261 cttcgcttcc cagaacgagg cgcccagctc gcggagcgcg aagctcggcc tggaccggcc
    19321 ctccgcttcg agcttcagcc cctgccggat accccaggcc gccgtcagga cgacgaggag
    19381 gaatccggga acgagcccgg cgaggtagag cgagtcggcc gggaccgagg cgacgacgct
    19441 gtagaggatg accggcagcg acggcgggaa gaggaggccg aggctcccgg cggccgtcac
    19501 gaggccgagg gagaagccct ccgggtagcc gtcgtcgcgg aggatcgggt agacgaggcc
    19561 gccgagcgcg atgatcgtga cacccgagcc gccggtgaac gtcgtgaaga gggcgcagac
    19621 cgccgcgacc atgacggcga tgccgcccgg catccagccg aagagcgcgc ggaagaagcg
    19681 gacgaggcgc gcggaggcgc ctccttccgc caggatgtag cctgcggccg tcaggagcgg
    19741 gatcgccggg agcgttggcg aggcgacgag gcggtagacc tctgccgata cggccgcgac
    19801 ggggacggcg tccttccaga agaggaggca ggcgagccct cccattgcga cgaagacggg
    19861 ggctccgagg agcgccgcga ggaggatgac cgcggcgagc ggaaggacga gcttcggcgc
    19921 gctcgcctcc ggcacgaggg agaggagcca gggcgaggcg agcgccgcga gggcggcgaa
    19981 ccggcccctg gccccgtccg aggccttcca ggcgaagcgg atcgccatca ggaggagggc
    20041 cgccggcatg acgagctcgg cgatccactc cgggaggccg aaggagagga ccttcgcctg
    20101 ctcgcggtcg gccttcacga ggccgacgct cgcccaggag aggacggcgc agacggcggc
    20161 cgcgacggag aacgcgaaga gccgggccag ccgccgggcc cggccgctcc cgaggagctc
    20221 ggcggtcgag agggtcaggt gggtcccctc gcgcgtcgcg aggaggccgc cgacgaaggc
    20281 gatccagagc gtgacgagct ggaggatcga cccggagccg gggacgtgga accccccgag
    20341 cggccggccg aacgcgtcga tcagcgggag gacggtcgcg accgcgagcg ccgcgacgag
    20401 gagcgactgc tccgccttcc cgaggagcgt gccggctctc acttcttcgg cgccgcggcc
    20461 ttcttcgccc ggaagtcggc caggatcttc cgtgcctcgt cgaaggcggc ctcggggacg
    20521 atcttgccgc ggaccttcgg ataggccgcc tcggcggccg cccgccagag cgcctccgtc
    20581 ttcgcgtcga tcgcgatcac gttcagcccg cgcttctgca tcgccgcgac gtcgcgtgcc
    20641 tcggccgcgc ggctctcgga ccggaggcgc gcgcccgcct ccgccgcggc ggcccgaagc
    20701 gccctctggt cctccggcga gatcttgtcc cacgtcgcct tgctgacgac ggtcgccccg
    20761 aggaggaggg cccacttcac gtcggtcagg ttcttcgcgt gctggtacca ctggaggagg
    20821 acggccgccg tcggcgtcgt cgggagggcg gagacgaggc cggtctggag cgcggtcgag
    20881 atctccgtcg acgggagcgg caccgggttg aagccggccc ctttccagat ctcgagggcg
    20941 tcggtgtcgt tcccccaggc gaagagcttg agcggcttga ggtcggaggg cgtcctcacg
    21001 ggcgtcttcg tgaagaagcg gacccagccg gcgtcggccc agttcaggac gacgaaccct
    21061 ttcgcggcga tcgacgcctc gagcttcggg ccgtagacgg cgaggacgtg gtcgacctcg
    21121 tcgtacgagg cgtagagcat cggcacctgg agcgccatga cggacgggtc gatcgcggag
    21181 acgccggagc tcgtcaggag gccggcgttc aggctcccga ggcgcatctt ccggacgacg
    21241 tccgcgtcgt cgcccgcgac gctcccgggg tagaggcgga cggtgacgcg cccctgcgtc
    21301 gccgtcttcc acttctcggc catctccttg aggatgaggt ggtagctcga gccgtcgggg
    21361 gcgagcgtcg ccatcttcac gacgacctgc gcgcgggccg gggaggcgac cgcgaggaaa
    21421 agggcgagcg cggggaggag gagccgccgt cgggcggcct gagggctgcg agctgtcatt
    21481 tcgttctact ccaggaagag ctcgtcggcg cgcgcgagga gccaggcggc ccggcgccgc
    21541 gcgacggtgt tggcgagccg aagctcgggg acggcgtccg ggtcgaccgc gagggccttc
    21601 tgcaggagct cctcgaactc gggccggttc tgggcgccga cggagacggt ctcggcgaag
    21661 gagacgtacg gggccgcgcg tctgccgtcg gaaagcgcga cggcgcgttc gaggtgcctg
    21721 cgggcgcgct cgacggagcc gccggctgcc gccggacggc ccccctcgaa cgagatgaag
    21781 aagtcgtgga tcgagccctt cccgaacgcc tcgtcgagct cgagggcgcg acgctcgagg
    21841 gcttcggcga gcccttggtc ggccgtcagc tcggcgtcct ccttggagag ggagatcgcc
    21901 gcgccccagg ccgcgcccgt ccagtagagg agggggacgt cggtcttcgt cgccgcggcg
    21961 agcgtcgccc cgcggcgcgc ctcgtcgcgc aggcccgcct cgaagcccgg gtggcgggcc
    22021 tcgaggcccc ggaggccgta ctggagcgct cgggcgtaga gcttccgggc ccgggcgcgg
    22081 agcgccgtcg cccgggcgag gtccttctcc tcgacgaagt cggcctcggc ctggacgaac
    22141 gcgaaggcgt actgcgtgaa gccgctcgac gcggcgagga ggagtccgtc gtgcttcggc
    22201 gtctcggcga ggagcgcctc catcgtcttg aggccgaacg gaatcgccgc cgcgacgagc
    22261 tcggggtcgt catccgtggc gtaggtgtcg ccgccggccg cgagggcgtt cgcgagggag
    22321 ttcacggcga cctttcggac cgagcagccc gaagcgagga gcgaggcggc gaggaagggc
    22381 gagagggctc cggcccagcg acgggtgcgt gccatccgtc acgagaataa accgcgcgga
    22441 ggggttcccg tctactccgt tgctgctaga gtaagtccca ccggcccatg gattcgagac
    22501 gcccggtgca tccgcgagga tgccggaggc gaaaggagcg atgatgtcgc agagagcggc
    22561 tctccgcggg ttcttcctct gcatccttgc cctcctgttc gggcggcctc tcctggggca
    22621 gtcggggacg atcaccttcg cgccggaccc tgtccaggtc cgcgtcgggg agatctcgca
    22681 ggtcgtcacg gcgacgatcg agtacctcgg gacgccaccg ggcggcgcgc agaccctgtc
    22741 cttcaacgga ggcgacggga tctcgggcgc gacgaccagc ccgccgacga tcacgttcac
    22801 tcccgtcgtg ggggggccga cgacggtcct gttccagctc gtcgcggcgg ccgacgcgtt
    22861 tccgggaccc ttcgaggcga gcgcttccac caccggcgcc ttcggcctga tgaactacga
    22921 gatcctgccc ttctccgtct cgccggactc cgttctcgtc ggggcgggca atacggtgag
    22981 cgggctcacg gcgacggtct cgtaccccga cttcacgccg acggggccgc agcagctcac
    23041 gttctccggc ctcccggcgg gcgcgagccc ggtcccgtca cccgtctcgt tcaccgtgac
    23101 gggaacgggc gcggtcgtcg ggttcggcat cgccacgagc ccggccacgc ctcccggaaa
    23161 ctacacggtc accgtcggca cgacgcccgt ggccaccggg accgacacgt tccagctgct
    23221 ggtcgccccc gggagctcca tctcgatctc gccgacctcc acgacggtgc tccccggcga
    23281 gacgacggcg gagctgacgg cgaccgtcaa cttcggtcct ccggggccga ccggaccgca
    23341 gcagctcgtc ttctcggggc tcccgccggg ggcgacttcg gttccctcgc cggtttcgtt
    23401 ctccatcatc cagatcccca cggtgaacgt gccgttctcc atcgcgacga gcgcggcgac
    23461 gcctccgggg agctacgtcg tgaccgtcgg cacgagcccg gcgagcgccg ggacggcctc
    23521 gttcgagctc gtcgtggccg cctcggggcg cctcgacctc tcgctcgcgt cgtcctcgct
    23581 cgacctctgt ccgggcggtg ccgccgtgga gaacgccgtg acggtcaccc ccgctgatgg
    23641 attcgagggg agcgcggagg tgacgttccc gctcctgccc gccgagctgg tcgtcactcc
    23701 gctctccgtt cccgtggatg ccatgccgcc ggcgcagacg gtgtcgttcg ccgtcgcggc
    23761 ccggccggac gcgacgccgg ggcagaaggt cgtcgtcgtc cgggtggcca gcccgttcgg
    23821 cgccggcgcc ctcacctcgt tcaccgtgaa cgtcgcggcg ccgggcttca cgccggctct
    23881 ctcgcccgaa gccctgacgc tggacgcggg aggcgggacg gagaaggtca tcgtctctct
    23941 cgcggcatcg gcctgccccg cgaccgcgga gacgatcgtc gtgacgccct cggggctccc
    24001 ggcgggcgtc acggtgacgc cgaggtcggc cgtcctgttc ggacccaccc atcctccggt
    24061 ggagttcacg ttcgtcgcgg cgtcctcggt gccggccggc acggccacgg cgacgttcgc
    24121 gttcgagccg tcgagcggcg gggcgaggac cctcccgttc ctcatcaccg tccagcgccc
    24181 gggacgcctc acggtcgtcg ccgagcgccc ggtcatggac gtctgcccgg gcggcggcgc
    24241 ggccccgaac acgctgacgg tcagcggcac tgacggctac acggggacgc cgaccgtgac
    24301 cttcccgggg ctccccgccg gactcacggc gacgcccgcc acgatcgagg tccagagcgt
    24361 cccgccggcg cgcgtcgtct cgttcactct ctccgcggcc gcgggcctcg ccgtcggcga
    24421 cctcacggtg accgtgaagg tggccgaccc caacggagtc gaggccacga cgacggtcgt
    24481 ggcccgcgtc ctcccgccgg acttcgttcc gtcggtcgcg cccgcgagtc tcgtcctgag
    24541 cgccggcggc gccgccggga ccatgacggc gtccctcacg ccgggaagct gcccgccgtc
    24601 gaccgacgtc acgatcacgc cgtcgggtct cccgccgggc gtcgtcgtca cgccgacctc
    24661 ggcggtcctg tcgccgccgg agctcggacc ggccctgttc acgttcctcg ccggcgccgg
    24721 cgccgagccc ggcgcgtcga cgatcacgtt cacgtacgcg tctcccgggg cgtctccgaa
    24781 gaccacgacg gcgacgatct ccgtctgcgg cgcgcccgcg gctccggact ctccgacgat
    24841 ccggcctgcc ggcaacccgc agggaccggt gacggccacc gatttcctcg acctctcgtg
    24901 gatggcgccg gactcgggct tcccggcgac ccgctacgac tggcggatca acggagggcc
    24961 ctggaccgcc gccgccggga cgtccgcggg ggcgccgccg cgaggagcgg tcgacccggt
    25021 ccagcttttc gtccgcgcgt tcgcctgtga ccccgagaag gggcccgggc cggaggcgtc
    25081 cagccccgtc tacgccctcg cggctccgca ggcgagcttc gccgtcccgg cgtcgatcgt
    25141 cgtcggccag ccggtcacgt tcaccgacac ctcctctccg caggcgacga gctggctctg
    25201 gttcccgggc gacgggatgc ccgcgacgac ggtccagtcg ccgacggtca cgttcccttc
    25261 ggccgggccg agggtgatcg tcctggtcgc gacgaacggc tcggggacct cgacgaagtc
    25321 gacgacggtg aacgtcctgc cggcttcggc cgtgcggacg gcggcgggct ggttcgcccg
    25381 caggctcgac cgccagccgg acggccggct cgcgctcgac gaggtcgacg tcgcccgggg
    25441 aacgacactc ctccttcgcc ggctctccgg agcgggggat gcggtcgcgt tcctgaggct
    25501 cgtggatccg accggagcga ccgtcgtcga gaggcggctc gtccttgccg cgggagagga
    25561 ggcgcgtcac gagctgtccg ccttcggcga agggagcttc cggatcgagc tcgtcggccc
    25621 cgagggcctc gaggcgaccg tggtgaccac ggccgtgccg actccggctc ccgagctccc
    25681 cgcgccgccg agggagcccc ggcgcatcga gccgaggcgg tagcggatcg gggaggctcg
    25741 ccacgagcct ccccgcggcg gctgcgccgc ctcccctcga gttcgcgtac cggagacgaa
    25801 aagagcccgg gccgtccggc ccgggctctt cgcatgggga gtcgaaagcc gcgcgccggc
    25861 gctgccgggc gcggaagaga tcagaacggc gggaagaaga ccttcaggac cttctcggtc
    25921 gtcagctcct cgtagccctg catcgccttc gccctctgcg cctcgagccg gtcgaggagc
    25981 gaaggctcgt gcgcttcgta gaggacgccc gtcgtgaggg tctccgtctc gcgcgtgtag
    26041 gcgaaggcgg cggctcggct gtgacggtcg tacccctcgg gaaggcctcg gagcttcgcc
    26101 ttcatcacct tgaactggtc gtcgccgcgc caggtgacgc agggcgacat cacgttcagg
    26161 aacgcgaagc cgcggtggct catgccggcg accatgaggt cgacgagacc tttcaggtcg
    26221 cccgagaagc cgcgggcgac ccaggtggcg ccgaaggaga tcgcgagctc gcacgggtcg
    26281 acgctcggct cggggttccc gaagaaggtc gacttcgtct tgtcgcccga gggcgtcgtg
    26341 ggagccgcct gtcccttcgt gagcccgtag atctcgttgt ccatcaggac gtagaagacg
    26401 tcgaggttcc ggcgagaggc gtgaaggaag tggttgccgc cgatcgcgag gccgtctccg
    26461 tcgccgccga ccgccacgac gttcagctcg gggcgggcga ccttgatgcc cgtcgcgatc
    26521 gggagcgcgc ggccgtggac gctgttgaag ccgtaggcct cgacgtagcc gggcagccgc
    26581 gaggagcagc cgatgccgga gacgacgacg gtgttccagg gctcgagctg cagctcggcc
    26641 atcgcgcggt acatcgcgga gacgacgccg aagtcgccgc acccggcgca ccagacgggc
    26701 ttgaggtcgc tcttgtagtc gcccggctgg aagcggggcg ggtcgggggt cgtcacgacg
    26761 ctgctcacgc ggtcacctcc tcgcggacgc cggatcccga aaggacgctc tcgatacggc
    26821 ccttcacctc ggacacggaa aggttcattc cgcccgaccg ggtgaagacg cgcgtcccgg
    26881 cggggaggtc gacgaaggtc ctcatgtagt tgtagagctg cccgacgaag ttcacctcga
    26941 ggaagacgag cgtcttcacg ctcgccgcgt attcgaggaa cgccttcttc gggaacggca
    27001 tgatgatcct ggggacgaac gcggagacgc tcacgccgtt cgccgccagc tccttcatcg
    27061 cctcctcgac ggggcccttc gaggagcccc agcagacgat gccgacgtcg gccttctccg
    27121 ggccgaggtg gacgtggagc gggtactttt cctcgacgaa gtcgagcttg gcgaacctct
    27181 tccggctcat cttctcgtgc atgacgtgcg aggaggcggg ccgggcggtc tcgtcgtgct
    27241 cgaggccgtt cgtctggtac atcccgccct tcgtcccggg gacgctcatc ggggagacgc
    27301 ccgtcggggt ctgcgcgtag cgcttgtagt cggcgagctc ctcggccgtc ggggtccggc
    27361 ggtcgatgac ctcgtggacg aggttcgcct tctcgaccgt ctgccggctc tggccgatcg
    27421 cctgatccga gaggacgatg accgggagct gagtctcctc ggcgatgttg aaggcgtcga
    27481 ccgtcgcgtg gaagcagtcc tccacgtcgg tcggcgcgag gacgatgcgc ggcgcgtcgc
    27541 cgtgcgatcc gaagacggcc atgtagaggt ccgactgctc cgacttcgtc ggcaggcccg
    27601 tcgaggggcc gccgcgctgc acgtcgagga tgacgaccgg gatctcggcc atcgaggcga
    27661 ggccgagcat ctcggccatg agggagaggc cgggaccgga cgtcgaggtc atcgacttca
    27721 cgccggcgaa ggagcccccg atgatcgcgc cgagcgcgga gagctcgtcc tcggtctgga
    27781 cgatccggcc gccggagcgg gggagccact cggagagaaa gtggaggacc tcggaggagg
    27841 gggtgatcgg gtagccggcg aagaagcggc agcccgcgtg cagcgccgcc acggcgcagg
    27901 cctcgttccc cgtcatgacg agcttgggcg cggagatcgc gtactcgagc ttccgcgcgg
    27961 agacgtcgac cccgatcgtc ccggcgaact cgcgcccggc ggcgaagccc ttgaggttcg
    28021 cctcgaggac gcccgccttc ttctttccga acttgaactc gacggccttc cgcaccgatt
    28081 cctgcgggat gccgaagagc tcggcgagga tgccgagcgt gacgatgttc ttcgcggcct
    28141 tcgttccggt cgccttcgtg gcgagctcga cgaacggcac cgggatccac gtcgcgttcg
    28201 tcgccgtcgg gatcttcacg tcctcgagcg gcgtcgggtc gctggcctcg tagaggatga
    28261 ccgcgtcggc cttcgggacg acctcgccct tgaaccgggt gaagtcggcc cagttgaaga
    28321 cgacgagcac gtcgaccgcg tcgccctggg cggagatcgg ctcagccgac atccggagcg
    28381 tgcaggaaga ctcgcctccc cggatctgcg gcccgtaggc ctccaccttg atgacgttca
    28441 ggccgtctcg ggcgccggcc tgggccatca tttcgcccat cgtcacgacg ccgtcgccgc
    28501 ccgagccgac catcgcgagc gtgaggtctc tcgtaggcat ctcggaaccc tccacgggcc
    28561 gcctcgcggc cccgaaaaag tcgctggagt ctctgcctgt cgggcgcgaa gtgccatgat
    28621 gccgtttcat cagggcccgc gccgcagagc ccactatacc ccggcctccc cgcgaggccc
    28681 cggcccagcg ggcgccctct ggcggtacca tccgcgccca tgaaggcgct tccgctccgc
    28741 accgcgaccg ccgccccgat cctcttcgcc gcgctcctcg ccgccggctg cagcggcaag
    28801 gaggcgccgc aggccccggc gaccgacgcc ggagctcccc tcgtcggagc ccacggcggc
    28861 gaagcggccg ctccggcggt cccgtcgatc cggggaaccg tcctcgagac gatggatgcc
    28921 gcgggctaca cctacctgaa gctgaagacg gccgagggcg agacgtgggc cgccgtgaac
    28981 gaggcgaagg tcgccgtcgg cgacgaggtc acggtcctcc agccgatgac gatggacggc
    29041 ttcgagagca agacgctgaa ccgcacgttc gagcggatcg tcttcgggac gctcgggccc
    29101 ggcacggccg ctcccgcgac cgcacccgga cccgccggcc ccgtcccggc ggcgatggcc
    29161 gcggcccacg cggccgccgc ggacggaccc gagacggccg agaagatctc cgtcgcaaag
    29221 gccgaggggc cggaaggacg caccatcgcg gagctcttcg ccgtgcgcgc cgccctcgcg
    29281 gggaagccgg tcgcggtgcg ggggaaggtc gtcaaggtga acctggggat catggggaag
    29341 aactggctcc acctgcgcga cggctccggg accgccgaag ggaaggacca cgacctgacc
    29401 gtgacgacgc aggacgccgt gtcgatgggc gacgtcgtcg tcgtgaaggg gacggtcggc
    29461 gtggaccgcg acttcgggtc gggctacacc tacagccttc tcgtcgagga cgcgaaggtc
    29521 acgaagtagg tccgtcgggc cctccgtccg ccttcccgaa ccagtgcgga tcggggaggc
    29581 ggaggccgag gtggaccgag gccatccgga ggaggacgat cgtcgcgaag cccgcggcgt
    29641 cggcgaacga ctgcctcacc tcgaggaagg gccccgccag gacgacccag cagccgacga
    29701 acgacgaggc ggcgtagagc gcccccggcc ggaagatggc cgggatctcc cggaccagga
    29761 cgtcgcgcag gacgccgccg aacgtccccg tgacgacgcc gaggagcgag gcggggagcg
    29821 gggggagccc cgcttcgagc gcgtagcgga cgccggtcag cgagaaaagg gcgaggccga
    29881 gcgcgtcgac gaagacggcc ttgcggtcga gcgccgcggc ggtggcgtag gcccgccggc
    29941 tgtagacgaa ggcgaggcag aggaggagcg tgccgaagac gtatccctcg ttcgccaccc
    30001 agaagagcgg cctccgctcc aggatcaggt cgcggagcgt tccgcctccg aaggccgtca
    30061 cgagcgcgag cgtgaacgtc ccgatcaggt ccatccgcgc ccgggcggcc gtgatcatcc
    30121 ccgagaccgt catcacgacg acggcggccg cctcgacggc gagcgtcagc tcggggagcg
    30181 cgggcttcac ggtgccggga ttgtcgcagg cgcggcggat cgccgcgggg acggagccat
    30241 ccgggcgagc ggcacgaggt agagcgcgaa gagggcgctc gtcgcgacga gggcgccgcg
    30301 gatgccggcg gcctccacga gggcgctccc gaggagcggg cctcccatca tcccgacggc
    30361 gtacgcgatg ttcacgaggg agaagacgcc cccgaacgag cggctcccca tccgctcgac
    30421 ggcgtcggcg aggccggggc tgacggggct caggacgaac gaggtcgtga cgccgagggc
    30481 ggccatcgcg aggccgaccg aacccttcga cgcgaggaag gccggcaccg gcaggaggaa
    30541 cgcggcgagg acatagccga cgaggagaac cttcttccgt cccacccggt cggagaggcg
    30601 gcccatcacc ggggaggtga ccgtgtgcgc cagcgcggcg acggcgaaga ggatcccgat
    30661 ctccttcggt ccggtccgga gggcggagtc gaggtggagc gggagcgtcg actccaggat
    30721 cgcccagagc gccgcgccga gcgccatcgc cccggccagc gtccggacgg ttccgtccgc
    30781 gagcagcccg cgcaggccga cgcgctctcc gcggggcgat tcgtcgtcgc cgaggaggag
    30841 aacgcgggcc acggcgtcgg cgaggacgat cgcgccggcg aggaggaagg ggctccgtgg
    30901 gccgaggctc tctgtcagga gcccggagac cggcgggccg aggaggacgc cgacgttcgc
    30961 ggcggcgaag acggtcgcca tcgcctgccc gcgcttctcc gacgggaacc cctcggcgag
    31021 aagagcgagg cccgtcgtcc aggtggcggc ggccgcgagc ccctggaggg cccgggcgac
    31081 gaggaggagc gggtacgacg cggagaaggc gaagaggaac gtcgtcgccc cgaggccgac
    31141 gagcccccag aggaacggcc ttcttcgtcc gatccggtcc gcgagccgcc cgagcgggta
    31201 cgtccccgcc aggagggcgc ccgcgtagac gccgaagagg accccgacct ccatctgcga
    31261 aaggccgaga gtcttcgcgt agtgcggcag gagcgggacg atcaggtagt agaggatcga
    31321 gtcggcgaag aaggcgaggc cgacgacgac gaggagagcc gtgggggagg tgcggcgggg
    31381 cacgggggga tggtagccgc acggaggtgc cgaggcgcgc ctcatcgggc gcgccccggc
    31441 gagagatcag aaggcgaaga ggagggatgc cgaggcgccg agcgcgtcgt cgtctccccg
    31501 gaccgacttc gcgagctcgc ccggaccgaa cctctcgctc gtgaggtcga ggtctcgata
    31561 cgtgtcgccc cggagccgga cctgcgcaac gtactcgaag gtcctcgtcg cggagggcgg
    31621 aggagcgggc gccgcgggat tctctcgtgc cacggcgcag ggggcggcgg cgaggacgag
    31681 aagcgccagc gttctccggg gcatgaggga ctcctttcgg cgatccggcg gggtacacga
    31741 ggcgtccccg ccgggccgtc cggaggatcg gccgcgggcc gtcacgctgg catgacatcc
    31801 atcatggcga ccgcgcgaac gcgctcggac ggtcctttcc gcgcttgacg cccggaggac
    31861 gccgggccag agtccccggg gtgagagcga gctacgaaat gcccagccgg cggggcaagg
    31921 tgcggcggcg cgggctcgcc ctcgccgaga cggcggccgg cctcctcctc gggaccgccc
    31981 tcggcgtggg cgcctatacc ttcgtctacg cgaagggcgc ctcctacctc tcgaatgacc
    32041 cgaacgcctg cgcgaactgc cacgtcatga cggagcagct cgacgggtgg tcgcgcggga
    32101 gccaccgggc cgtcgcgacc tgcaacgact gtcacacgcc gccgggcttc gtcgcgaagt
    32161 acgcgacgaa ggcctcgaac gggttctggc actcgttcgc gttcacgtcg gggcgcttcc
    32221 ccgatccgat ccagatcacc gaccgcaacc accgcgtgac cgagagggcc tgccggaagt
    32281 gtcacgcgga cgtcgtccac gacatcgacc gtgtgactcg cgaggggcgg gagctctcct
    32341 gcatctcgtg ccacaggtcc gtcggccacc cgcactgagc gcctccacgg gaagcgaggg
    32401 agagagatga ccgagaagaa gagcagcacc gcgaggacga tcctcgtgat cgcggcgatc
    32461 gccgcgttcg cggccgtcgg cgtgacggcg ctcctcgtca acatcatgga gcacaagcag
    32521 gaggcgcgga acccgttctt ccgcgtcgtc gagctcgacg acgacaccga ggatccggcg
    32581 atctggggga agaacttccc ggtccagtac gacacctaca agcggaccgt cgacatggtg
    32641 cggacgaagt tcggaggcag cgaggcgctg ccgaggacgc cgacggagaa ggacccgcgc
    32701 acgcaggtga cgacctcgaa gatcgaggag gacccgcgcc tgaagcggat gtggctcgga
    32761 tacgccttcg cggtcgactt ccgcgaggag cggggccacg cctacatgct cgacgaccag
    32821 acgttcacgg agcgccagca ggtcgtccag cagccgggga cctgcatgaa ctgccacgcc
    32881 tcgacctacg tcgcgatgaa gaagctcggc aatggcgaca tcgtgaaggg cttcgaggcg
    32941 atcaaccaga tgccgtactt cgacgcgcgc aagcacgtga agcacccggt cgcctgcatc
    33001 gactgccacg actcgaagac gatggccctc cgcgtgacgc gtcccgcgtt catggaggga
    33061 atccgggcct acaaggcgtc gcagggggtc ccggactacg acgtgaacac gatggcgacc
    33121 cgccaggaga tgcggaccta cgtctgcggc cagtgccacg tggagtacta cttcaaggga
    33181 gccgagaagc ggctcacgtt cccgtggcac aaggggctga aggccgacga gatcctcgcc
    33241 tactacgacg aggtcgggtt caaagactgg acgcacgcgg agaccggctc cccgaacctg
    33301 aaggcccagc accccgagtt cgagatgtac aaccagggga tccacgcccg gtccggcgtg
    33361 gcctgcgccg actgccacat gccgtacacg aaggtcgggg cgatgaaggt ctccgaccat
    33421 cacgtgagaa gtcccctcct caacatcaac aaggcctgcc agacctgcca ccgctggtcg
    33481 gaggaggagc tgaaggcgcg ggccgagacg atccagtcgc ggcacatgga gatgcggaac
    33541 ctggccatgg acgcgctgat gtcgctcatc gacgacgtga aggcggcgaa ggaggccggg
    33601 aagagcgacg aggagctcgc ggtcgcccgc gacttccagc gccgggcgac gttcctcctc
    33661 gacttcgccg aggccgagaa ctccaccggc ttccacgcgc cgcaggaagg ggcccgcatc
    33721 ctcgcgcagt cgatcgacgc ctcgcggaag ggccagctcg cggtccgagg cgcgctcccc
    33781 gcgacgccgc agccgctcca gaaggcggag gcgccggccg cgccacaggc ccccgggaag
    33841 gtggcgctcc tctcggcgcg ctgacggccg gcggcgaacc agtcgcttcc gagacggccc
    33901 tcggggagac tcgggggcct ttcgcgtgtg tgcggcctcg cgaggcgaga gaatcgcggc
    33961 atggacgccg ccgcctactc ggccttcacc cgggagctcg cccgccggct cggcgccgag
    34021 ccgcgcgtcg tcggcctcgt cgccctcggc tcgatgtcgg gggacggtcc gccggcggac
    34081 gacttctcgg accacgactt cttcgtcgtg acccggagcg gcgtgcagga ggcgttccgc
    34141 tcggacctcg gatggctccc cggcgcggag cgggcggtcc tcgtccaccg cgagacggag
    34201 cacgggctga aggtggtcct cgacgacggc cacctcctcg agctcgccgt cttcgacctc
    34261 gaggagctgc gcgtcgcccg cgcgaaccgc tacctcgtcc tcctcgatcg cggcggcgtc
    34321 gcggagcgga tggccgccgt cgccgcgaag cctgcggagg cgccgacgga ccgctggctc
    34381 tctgggcagc tcctcgggaa cctcctcgtc gggggaggcc gcgcggcgcg cggcgagctc
    34441 ctctcggctc gcctcttcgt cgcgggccac gcgacggggc acctgctccg gctcctctcg
    34501 cgccatgtcc cgccggaggc tcccgtcccg ctcgacgacc tcgacccgtt ccgccgcttc
    34561 gagcgcgcgt ttccgattct cggcgccgag ctgaacgcgg ccctctcgct cccgccgctc
    34621 ccgggcgccc tcgccctcct cgacctcgcg tcgcgcgagc tcgggggccg cgtcgccgcg
    34681 tggccggccg aggcggcgga aacggtccgg aggaggcttt cggggatgcg ggatgggggc
    34741 gacgccgggt agaatccccg gttgccatgc tgttcaagac catcatcgag cctttccgca
    34801 tcaagtcggt cgaagccgtc aagttcacga cgcgcgagga gcgcgagaag gcgctcgtcg
    34861 aggcggggta caacgtgttc ctcgtcccgg cggaggacgt cctcatcgat ctcctgaccg
    34921 actccggcac gggcgcgatg tcgtccgcgc agtggggcgc gctcatgcag ggggacgaga
    34981 gctacgccgg gagccgctcc ttcttccgct tccgcgacgt ggtgcaggac ctgacgggct
    35041 tcaagcacgt catcccgacc caccaggggc gcgcggcgga gcggatcctc ttccacaccg
    35101 tcctgaagcc cgggatggtc gtcccgagca acacgcactt cgacacgacc cgggcgaaca
    35161 tcgaggtgga gcaggccgag gcccgcgacc tcgtcatcgc ggaggggaag gtcccctcgc
    35221 tgatccaccc gttcaagggg aacgtcgacc tcgcgaagct cgagaagacg ctcgccgagg
    35281 acggcgaccg cgtcccgctc gtgatggtga ccgtcacgaa caactcgggc ggcgggcagc
    35341 ccgtctccct cgagaacctc cgcggcgtcc gggcgctctg cgacacgtac ggcaagccct
    35401 tcttcctcga cgcgtgccgc ttcgccgaga acgcctggtt catcaagcag cgcgagccgg
    35461 ggcagtccga ccggacgccg aaggcgatcg cgcaggagat gttctctctc gccgacggct
    35521 gcacgatgtc ggcgaagaag gacggcctcg cgaacatcgg cggcttcctc gcgatgaacg
    35581 acgacgagct ggccgcctcg tgccgcagcc tcctgatcct cacggaaggc ttcccgacgt
    35641 acggcggcct cgccggctac gacctcgagg cgatcgcccg cggcctcgag gaggtcgtcg
    35701 aagagccgta cctgcgctac cgcatccgct cgacggccta cctcgccgac aagctgacgg
    35761 ccggcggcgt cccgatcatc cagccccccg gcgggcacgc ggtctacctc gacgcgcggg
    35821 cgatgctccc gcacgtgacg ccgctccagt accccggcat cgcgatcgcg aacgcgctct
    35881 acctcgaggc ggggatccgg ggcgtcgaga tcgggaccgt gatgttcggc ctccaccctg
    35941 acgggacgga ggcgccgggg ccgatggacc tcgtccggct cgcgatcccg cgccgcgtct
    36001 acacgcagtc gcacatcgac ctcgtcgccg aaggggtcct cgaggtcttc cggaagcgcg
    36061 aggagctgcg cggctaccgg atcaccgagg ccccgaaggt cctccggcac ttctcggcgc
    36121 gcttcgcgcc gctctgagcc ggcgcggggc cgcgccccgc gggaccggga gagacgaccc
    36181 cgcgccggag tatcggcgcg gggagcggcc tacggccggg atccctcctc gccggggcgc
    36241 agcgttcgcg tttcctcgtg gtactcgtcg tcgacgttga cggcccagag gtcgttcgag
    36301 gcgccgtaga ggttcctcac cgcgtagaga cccgtcacca tcgcgtggtc ctggttgttg
    36361 tagcggtgca tcccgttccg tccgacctgc tggaggttcg ggaggcgctc cgcgaagaag
    36421 cgccggacga cgtcgacttt ctcggagtag cccgggtcgt acatcgggta ggtcttcggc
    36481 atcctcacga cggcgccgtc gaggacgcgc gccccctcgg ggacgaggcc ggtcttcgtg
    36541 atctccgacc ggcccgtctc gaggagctcg tcgtcgggcg cggaccagac cgggtcggtg
    36601 tcgaagcaga aatactcgag gccgaggcac gtgacgccgg ggcgggggac catcgcggcc
    36661 gaccagttcc cgaagttctg gacgcggccg atccgcacct cgggactgtg gatgtagacc
    36721 cagttgtcgg ggaagaagcg ctccccctcg aacatcagcg cgacgaggat gtagtcgcgg
    36781 tagcggagag cgcgggcggc ttcgaggacg tccggcggcg gcggcgggtc gagcgcgagg
    36841 acggcgtcgg agagggccat cgaggagacg taggcgtcgc ccgtaaaggt ttcccgtccg
    36901 gccgagccgt ccgtctcgac cgccgtgacg cccctgcctt cctgccaggc gatccgcgcc
    36961 acgctccgcc cgaggaggac tcgtgagccc cgctcctcga ggcgggcggt gaagcgctcc
    37021 cacatctgcc ccgggccgag gcgcgggtag tcgaactcct cgatcagcgt cttgacgctc
    37081 cggtcgggct tcaccgcgtg ccagacggcc ttccagagcg agaggccgcg gatgcgctgg
    37141 gcggcccagt cggcccggat ctgcgtgcag gggacgcccc agaccttctc ggtgtaggtc
    37201 ttgaagaaga tcgagaagag gcgccgaccg aagcggttcg agacccacgt ctcgaagtcc
    37261 tcctcggggc tgcgcggggc gaggcgggcc gcgaggaagc tgaggccgca gcgaaagctc
    37321 tcgatcgggc cgaggccgaa gaacgcttcg aacggcttca gcgggtagga gaagagcttc
    37381 cggcggtaga ggatgcgcga ctgccgcggg acgcgcagga aatcgtccgc gagcatctcg
    37441 tgccagagct cctcgacctc gcggaccttc gtgaagaacc ggtggccgcc gatgtcgaac
    37501 cggtacccgc gccgctcgac ggtccgggcg atcccgccga cgaggtcgtc gcgctcgagg
    37561 acggtcgcgg ttcccccgtg tcgggcgttc tcccaggccg cggtgagccc cgccggtccg
    37621 gcgccgacga cgacgagcga gagcgggtgc gggtcggtcg gggtgtcggt catctgcgtg
    37681 cgctctcccg ggggacggtg ccgatgcgag gtccggcgac gacccctcgc ggggagtgtc
    37741 gcacggcgcg gcgacgctcc gcgccggctc agatcgagat cgagcccttc cggaaagccg
    37801 tcttgccgag gcgcgcgtcg acgacgacgg gtcggccggt ccgggcgagg cggaacgcct
    37861 ccgcaaggac ggggccgcgg cgtgtcgggt cgtcgaggag gagcccctcg ccgccgaggc
    37921 cgcgggccgc cgcgtggtag tcagtcgagc gcaggacggt cccgacgtcg tcgccgagca
    37981 cctccacctg ctcgcgggcg atctgcgtcc atgcggcgtc gttcccgatc acggcgacga
    38041 cgcccaggcc gtgccggacg aacgtgtcga cctcgacgag gccgtagccg gccgagccgt
    38101 ctccccagag gaggaggacg ggcgctccgg ggcggcagag cgccgccccg agcgcgaagc
    38161 cggcgccgac gccgagcgtt ccgaagacgc cgggatcgag ccaggagagg ggcgcccgcg
    38221 gccggaggac gtaggaagcc gtggcgacga agtcccctcc gtcggcgacg acgaggccgt
    38281 cttccggaag ggcctcctcg aggctgcggc agagggcgac cggatccagc gcgcctcgcg
    38341 ccccatccgg cgcggcggag atctccgcgc cgcgggtggc gtcgcgttcc cgtaggcgcg
    38401 cgagccactc cgggcgaggg aagccgcccg gcacggcgtc ggcgagagcc cggaggaaga
    38461 ggcctgggtc gcctccgacg tcgagtgtcg gccgacggtt gagtgcccgg gcggcggagt
    38521 cgcgatttgc cgagatgacg acggcgtccg gccggatctg ccggccgtaa tcgagccgga
    38581 agtcgcacgg gacgcccgcg aggaggacga ggtcggcctc gcggagggtg tcggtccgcc
    38641 ggtggcggca ctgcaggaga gaagcggggc cgaggaggcc gcgcgccatc cccgagaggt
    38701 agacggggag gccgagctcc gcgaccgcgc gcgcgagcgc gccggccccg gccgggtcga
    38761 ggagcgcgcc gctcccgagg agggcgacgg gacgttcgct tcgccccagg aggagcgccg
    38821 cggtcccgac gctcccggct tcggggggag gaggaggagc ggacgccggc ccacgagcgt
    38881 cgaccccatc gaccccggtg aagagcgcgt cgaggtgcct gtcgagatac cagcgcgtcg
    38941 ctttcgcgac gagtcccttc ggcgtccccg ccttcgaggc gtaccaaccg cggacgaccc
    39001 gctcggggta gaggaggtcg acggggcact ccacgaagac gggtccggga acgccgtctc
    39061 gggccacggc gaaggcctct tccagcgccg ggacgaggtc gcggagcttc gagacgcggt
    39121 acgcgcgctt gacgacgggg gcgacgaggg cgatctggtc gacgtcctgg agggcgccgc
    39181 ggccgcgcag gacggttgcg gtggcgccgc cgaggagcac cagcggggtc tgggcgaggc
    39241 gcgcgttctg gagggcggtc aacgcgttcg tgacgccggg cccggcggtc accgcggcga
    39301 cgccggggac gccggtgagt cgcgccacgg cgtcggcggc gaagacggcc gtcgcctcgt
    39361 gtcgggtgtc gacgacccgc accccgcgcg ccttcgccgc ggcgaggatg ggggagatgt
    39421 gaccgccgca gagcgtgaag aggaatcgga cgccgtggcg gccgaggact tcggccacga
    39481 gctccccgcc ggacgcctgc ggcgcccgcg ggaccgtgcg gtctgctccg tgtcgcgcca
    39541 cgagtgtcct cctgcgcgga ggttagcagc ccgggcgggc tgcccggagc agctccgcga
    39601 gccgcgtgac gcggctcccc gcggaggagc gccgggcggc gacggcgatg gccctccggt
    39661 cgccgacgag aacgacgagc ttccggcccc gcgtcatcgc cgtgtagagg aggttccgct
    39721 cgagcatggc gtagtgggtc atcgcgagcg ggatgacgac cgccgggtac tccgacccct
    39781 gggacttgtg gatcgtcgtc gcccacgcga gctggaggag gtccagctcg tcgaagccgt
    39841 acgtcacctc gcggccgtcg aaggcgacgc ggagctcgcc ctcgccggga tcgacggagg
    39901 tcacgaggcc gaggtcgccg ttgtagacct cgcggtcata gtcgttctcc acctgcatga
    39961 cgcggtcgcc cggggcgagc tccattccga agcgggagac gcggggcgcg ccgccggggt
    40021 tcagcgcctt cccgagcgcc tcgttgagcg cgcgtgcgcc gagcggcccg cgacggaccg
    40081 ggacgaggac ctggacgtcc cgtcgcgggt cgaggccgaa gcggcgcggg atccgctcgg
    40141 cgacgacctc cacgacgcgc cgcgccgcct cctcgggctc cttcgcgtcg aagaagaaga
    40201 ggtcggtccc ggccgggttc gtcaggtcgg ggaggtgccc ctcgcggacg cggtgggccc
    40261 cgacgacgat ccggctctcg gccgcctgcc ggaagacctc ggtgaggcgg acggagggga
    40321 cggcgtctga ggagagcagg tcgcgcagga cctggcccgg cccgacgctc gggagctgat
    40381 ccgcgtcgcc gacgaggagc agggccgcac cgttcgggac ggcctcgagg agagcccggg
    40441 ccagcaggac gtcgaccatc gagacctcgt cgacgacgac gaggtccgcg tcgagcgggt
    40501 tgtcacgccc gcggcggaac cgcccggtcg acgggtcgac ctcgagcagg cggtggatcg
    40561 tcttcgcctc cgagcgcgtg ctctcggaga gccgcttcgc ggcccgcccc gtcggcgcgg
    40621 cgagggcgat ccggagcgag gcgtcgagga cgatccggag gagggcgtcg acgagcgtcg
    40681 tcttcccgac gccgggtccc cccgtgagga cgaggacctt cgagcgcagg aacgtggcga
    40741 cggccgcacg ctggctcgcg gagagggtga ttccggtcct ctcctccacc cacgcgaccg
    40801 cctcctccgg cttcacccgt cgccacgggg tcgtcccgga ggcgagcgtc gcgagcctct
    40861 gcgccacggc gcgctcggcc gcgtggagag ccggcaggaa gacgcacggc cggcctcccg
    40921 cctcttcggg gacgaggcgt ttctcggcga gctccagctc gagcgcctcg gcgacctggg
    40981 tctcggggcg gccgagcagc tcggcggcgc gcgggatcaa cgtctcgcgc gggaggccgc
    41041 agtgcccctc gtcgagggcg tccgcgagga cgtgggagag gcctgcccgg acgcggatca
    41101 tcgcgtcctc cgggatgccg agcctcctcg cgagggcgtc ggccgtgacg aagccgatgc
    41161 cacggacgtc gcgggcgagg cggtacgggt cctccgagac gaccgcgatc gcgtcggcgc
    41221 cgtaggtccg gtagatcctc acggcgcgcg aggtgccgac gccgtgggcg tggaggaaga
    41281 gcatgatctc gcggaccgcc ttctgctcgg cccagctcgc gacgatccgc tccgcgcgga
    41341 cggggccgac gccgggcacc tcgaggagcc gtgccggcgc ggtctcgatc acctcgaaga
    41401 cgccggtccc gaacgccgcg acgagccgcc gcgcgaagtg cggtccgacg ccgcggacga
    41461 ggcccgaagc gaggaagcgc tcgatcccct ccgccgaggt cggggccacc gtcgtcagcg
    41521 attcggcccg gaactggggc ccgtgcttcg gatcgttccc ccagcttccg gaggcgcgaa
    41581 gctcctcgcc ggcggagatc gccgcggcgt ggccgacgac ggtcaccgcc tcgcgcctcc
    41641 cgcgcacgcg gacgcgaagg acggcgaacc cggtctcctc cgagtggaac gtcacccgct
    41701 cgacctggcc cgcgaggagc tcccgatccg gcgacgcacc ttcggccttc atgcggcgag
    41761 gatcgtacgg gagagaatgg ccggcgtgag gaacgtccgc gcggtccccg ccctcctcgc
    41821 cgcccttctc gtcgccgcgg cggcgccggc gcaggaaccc tcgcggaggg ggagcatcgc
    41881 cgcgaaggcg ggcgtctcca aggtcctcgt cgacgcgcac gtcctcggtc ccggcgggag
    41941 gacgctcctc ggcctgaccc cctccgactt cctcctccgg gtcggcgggg aggaggtgcc
    42001 ggtggagggc gtggagttcg tcccggcgga gcggtcggag cttccgcccc ggcccgcggg
    42061 ccaggcggtc gagccgggga ccccggagtt ccccgacggg cggctgatcg tcctgttctt
    42121 ccaggccgac ttcggacggt atcgcgtgaa ggggcagatg cggatggcga acgagatggt
    42181 tcgcttcctc gaccgcctcc tgccgacgga ccgcgtcgcg gtcgtctcgt acgactcgca
    42241 cctgaagctc cggctcgact tcaccgacga ccgaaaggcg atccaccgcg ccctcttcga
    42301 cacgctgaag atcggcgggg cgcccgaccc gccgcccgcg agcccgttcc cgtccctggc
    42361 ggcgcacttc gacgtcgagg aggcgcgggc cgccgcgaac gtggagcggg ggctctacgt
    42421 cgtcgcgaag gcgctggaga agatcccggg cggaaagtcg atgttcttcc tcggctgggg
    42481 gctccacgtg aaccgggcgc cggccgactg gaagtggcac gccctggccg tgaacgcgct
    42541 ccaggtcgcg cgcgtgaacg tcttcacgct cgacgtctcg gacgccgact ggcacacgct
    42601 cgaggggacg ctccggaagc tcgccgacct gaccggcggg acctacgcga agacgcacgt
    42661 cttctcgagc caggcgctcg acctcgcgtt ccgcgcgatc gaggggcgct acgtcgtcgt
    42721 cttcgaccgc cccgaccgcc cgcccggccg ccacgcgatc gacctgacgc tccggatgaa
    42781 gaaggggcgg gtcctcgccc gccccttctt cgacgatccc tgagggcgcc cgtcccgcgg
    42841 gccccccgcc tcgcgcgcct cagacggcgg cgggggcctc cttcgcctcc tgcttctcga
    42901 cccagcggtt gaacgcccac atggcgacgg cggagaggaa gccgatcgcc atcaggagga
    42961 gccagccgcg cgtcgcggcg gcgcggttca gctcgaggag gccgtcggcg cgcgtcgtgg
    43021 cgcccttcgc catgacgtcg ttgaagatcc aggcgccgac cgggccgccc gtgagcgagc
    43081 cgatcgcgag cgggaggttc gcatagccga ggaagaggcc ctcctgcccc ttcggggcga
    43141 gggctccgat gtactcgtac atccgcggcg aggtgtagag ctcgccgaac gcgatgaggc
    43201 ccaccgtcag gatgatgaag atcgaggcga tcggaaggag gtcggccacg ggggcgcgga
    43261 gcccgccggc ggaccagagc ggccagaggt tgatccccat cgagacgccg atgatcacgc
    43321 agc
//
LOCUS       DAOONL010000022        42759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_661_length_42759_cov_19.424808, whole genome shotgun sequence.
ACCESSION   DAOONL010000022 DAOONL010000000
VERSION     DAOONL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 42759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42759
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_42759_cov_19.424808"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..320)
                     /locus_tag="PLP50_05495"
     CDS             complement(66..320)
                     /locus_tag="PLP50_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51041.1"
                     /translation="MTDPEEREARLEAAEALRGLARNMDTMAAQFASMTEKMGMHGHL
                     MPLSVELRTPERLRQLYSDVLSVADDLRRIAEEITEARAD"
     gene            complement(399..677)
                     /locus_tag="PLP50_05500"
     CDS             complement(399..677)
                     /locus_tag="PLP50_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51042.1"
                     /translation="MKPIEVRTVRSSLSKKGFEGKQADHLFYFFYFEGKKTAWWVKIS
                     HGAKEMRQREIKANAMVLGLSASDFHGVLSCEKDHQWLLAKYRAAQGT"
     gene            complement(674..1126)
                     /locus_tag="PLP50_05505"
     CDS             complement(674..1126)
                     /locus_tag="PLP50_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPA51043.1"
                     /translation="MSLADRIQTAGAGEEALYTRLHGETFSPRRSRLLGTMSSAESMP
                     DETAMDAVRYAPIVSWLTELVGGPDEATLRLSCPVRFDVTVEPDSEGFIAFMPFLASA
                     GTGDTYEEALRDLSESIRGLKAALDSEPLELLAPDAARIKARLAYLLG"
     gene            complement(1123..1845)
                     /locus_tag="PLP50_05510"
     CDS             complement(1123..1845)
                     /locus_tag="PLP50_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HPA51044.1"
                     /translation="MRESSRLANAPLVEVVFEIRFPGNFAVYAGLDRFQRALGPEFPS
                     LFVPKAAAGEPLALKPFQWKSEDGSRSVNVALNVFSYMSRRYGVFEEFKTEFRRLSSM
                     FFEIHRPDSLTRLGLRYVNIFPAMECTAEALHPWLELGLRLPALGESEASEVAAAFLL
                     KRPGGTLRLAFGEAAPSDFTPIGPAGVRLTGFALDFDFFRVGPAVVGDIDPFLDEGHQ
                     TIETLFFSMVKPEALRVMEGKG"
     gene            complement(2057..2134)
                     /locus_tag="PLP50_05515"
     tRNA            complement(2057..2134)
                     /locus_tag="PLP50_05515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2097..2099),aa:Arg,seq:ccg)
     gene            complement(2158..2233)
                     /locus_tag="PLP50_05520"
     tRNA            complement(2158..2233)
                     /locus_tag="PLP50_05520"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:His,seq:gtg)
     gene            2311..3621
                     /locus_tag="PLP50_05525"
     CDS             2311..3621
                     /locus_tag="PLP50_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPA51045.1"
                     /translation="MTSPDGATPSASSEGSLSNRILLGLLLGAICGVGANLLLVPRFP
                     DAVEAANRWLFVPVGQVFLRMLFMVVIPLVFASLALGVASLGDLKRLGRVGGRTMLYF
                     LSVTAFSVAIGLVLVNVLKPGEGLPAETREKLLETYRGEAASRMESAEKADFGPSLLV
                     NIVPRNPVAAAAKGDMLPVIFFALLFGIGVTKLSAEKNALWMKLLEGLADVTVVIIGM
                     ALKLAPFGVFGLIFSVTSRFGFDILRQLLFFVVTVLLGLSLLIFGVYPLYLKLRAGVS
                     PIAFLRKMRPVMITAFSTSSSSATLPTTIEVSERELGVPPQIAGFVLPLGATMNMNGT
                     ALFEGITCVFLAQVFGVHLSLGQQLFVIVLAVLTAIGVAGVPGGSIPLLVLVLTSVGV
                     PAEGIAVIIGVDRLLDMARTVPNVVGDASCAVYVAKKEGFELKI"
     gene            3624..4397
                     /locus_tag="PLP50_05530"
     CDS             3624..4397
                     /locus_tag="PLP50_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009334092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HPA51046.1"
                     /translation="MTFTHALVRPPASTFADGLTTAGLGSPDLPLALAQHEAYVRALE
                     ACGLSVTRLPADDRFPDSTFVEDTAVLARGLAVLCRPGAPSRAGEVEAIRTALESFFP
                     SAVAIEAPGTVDGGDVCEAGDHVFVGISERTNETGARQLAAHLATRGLGTTLVDVRGI
                     PGLLHLKSGIAWLGERTLALARPFAGRPEFAGWDVLDVPAGEEYAANAVLVNGHVLVA
                     TGYPRFAAAVSALGLPVLTLDMSEFRKLDGGLSCLSLRF"
     assembly_gap    4800..4899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4938..6266
                     /locus_tag="PLP50_05535"
     CDS             4938..6266
                     /locus_tag="PLP50_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51047.1"
                     /translation="MPGKKSEVRAWLAYDWANSAWAMTVAVAVLPVYFAGTVVPKGGV
                     ELLGFRIDATVLWGYVNSASTLAVFLTAPILGAIADRLGARRKLLTAFGLAGGLLTML
                     LAACGPGDVATTLVIYALSQIAFVSANVFYDSFLPHLAKDEELDRLSARGFAVGYVGG
                     SLHFVLALILMATHARWGLTAEAAARIALGSAGLWWAGFGAITFLGLREPPREGERPG
                     LLRAVREGIVDTLATTRRVAKVKPVLLFLLAFVLYNDGVQTVIKMASLYGTEEVGVGL
                     PMMMGTLLGIQLLAMVGALLFARLAAAWGTKRALVLAVALWLVAVGWAYLLTTPAGFV
                     VLAALAGLVLGAVQALSRSLFAAMIPRESSAELFGFFSVFNKLAGVLGTFLFATVRQA
                     TGTSRLAILSVAVLFVAGLVLLLRVNEAEARARRDELRGDGATTPTRRGS"
     gene            complement(6238..8766)
                     /locus_tag="PLP50_05540"
     CDS             complement(6238..8766)
                     /locus_tag="PLP50_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPA51048.1"
                     /translation="MRALRLRSALAAAFVLSLALPAAAAPPSPAEHLRLPVGADRTLA
                     DWGQIVSYFRALDAASDRVEVTTIGTSTRGEEMILAAISSPENLANQRRLREVARRLA
                     DPRGLSEAEATALVGEGKAVLLVTCGIHASEIGASQMAMEWAHALATAEDAETKRRLS
                     EVVLLLVPSLNPDGHRMEVDWYRKNLGTRWEGSRMPWLYHHYVGHDNNRDFAQLTQAE
                     TKAISRVLYHDWFPQVFLDEHQMGTSGPRMFVPPYADPLDPDIDPLVIREVNMIGSLM
                     SMRLEQAGKSGVISAWAFDVSWPGGSKNTAWWKNVTGVLTEVASVRLATPIEVPPTEL
                     RGGGKGLVEYGPQVNFPNPWRGGTWRLRDVMDYERVASDAIHEYCATHREDVLRNMLA
                     KARASIAAAAPGEGYRIPMAQRDPATARALAALLVEHGVEVKADSTGDAWVPLAQPYG
                     RFVREMLEVQRFPEVKLVPGKEIVAPYDVTAWSLPLSMGVTVEKGRLPESLRPLGATG
                     PVPSLPSGTTAVALGPESPERWKVVAAARKGGAVSILPAADGALAAGTIVLDAAAAEA
                     ASRAAADLGLSLAPLAAAPAGARPFRAPRVGLYKPWTASMDEGWTRFLLERYGFAPLT
                     LDNAALRAGKLAARLDVIVLPDVDKEVIADGKPKREEGAMRYFPEPRPEYAGGLGKEG
                     AAALRAFVEGGGTLVALGSSTEYLVEELGLPVRNALARVKSDEFLCPGGLVRLDVSPT
                     HPVTWGLPTSVPGFLDGPLAFQTTIPGAEMTREVLAAYPADGRDVLVAGWIRGEEKLA
                     RNAAAVALTLGKGKVVLLGFRPQHRAQTNATFPFLFNSLAWSVL"
     gene            complement(8796..9215)
                     /locus_tag="PLP50_05545"
     CDS             complement(8796..9215)
                     /locus_tag="PLP50_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51049.1"
                     /translation="MRLFQDVELNDAQANAVGRAMLAVARADGAADPREVALIEELAP
                     VDTTASDPSPAELAETLPEPKGRELLLQSALLVALVDGNYSAAERAVIGRFAETFGVG
                     EPRLEELASEVKAFLMAPLLPLANTPAVLDVSRKLKF"
     gene            complement(9318..10235)
                     /locus_tag="PLP50_05550"
     CDS             complement(9318..10235)
                     /locus_tag="PLP50_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPA51050.1"
                     /translation="MTAARRRGGCGRALALLAGLAFLFALAFLWLRYGRTPEAPPTPT
                     PTPVPTIAAPTPVPAPTAVPRPTSVPPDFEPAAGTGRGAIAVVIDDVGNADGSLARLE
                     RLSGPLAIAVLPDAPLAREAAALAKRKGWDLLVHLPMAGSRGPYEPGTISSDDDDATI
                     ARRVEEAIAAVPGAIGLNNHQGSVATADRRVVRAVLSVVRTKGLFFLDSKTSAASVAT
                     EEARALGLATIPRDVFLDDARAEAAAIGGAPEALGTAFARALSTAATKGHAVVIGHPH
                     AATVDFLELRFRFLEKSGVLRVRVSELVD"
     gene            complement(10232..11551)
                     /locus_tag="PLP50_05555"
     CDS             complement(10232..11551)
                     /locus_tag="PLP50_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA51051.1"
                     /translation="MTKSRLASFLGSLALLGTLFVVLPGLGADEKKGKGAKDGLYQPL
                     GLFTEVLSLVRGNYVEKVEVRPLLAGAFSGMTEAMDPFSEYVAPEKMAAFEAARAARE
                     KTDHVESGLVLARRSGYPVVVTAVPGSPAAAAGIRTDDLIEKVGDVSARSLALWEVEA
                     ALSGKPGGKGTVLVVREEKPRRRTLEIVRASWTPSLPSAERVSGELVVKVPAFGPGTA
                     DALAKILEPLDRTKSLVLDLRGNAWGTWEEAARTAALLGPPGPIGELKGRKIQTRTFT
                     VEAGRRLHDGGLVLLVDSGTGGPAELFAAALREGAARELGVDVGAAARREEKSAEAFA
                     PEDPGAAVAVPDKAKLRIRVVGEPTFGMGLTQEVVRLASGGALRITVGKVRTPAGRTL
                     SPRGLAPDDRVFHVLPEEGEEGKLPDPFLERALKVVAELPAPRARAS"
     gene            complement(11635..12726)
                     /locus_tag="PLP50_05560"
     CDS             complement(11635..12726)
                     /locus_tag="PLP50_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPA51052.1"
                     /translation="MTRDAAGTDRRRELEALRRRLAGLKTRYEETRQRAVDVRQQLEA
                     AEANLELRTTERRILELRAAETERAAAEAQAERDHARRQSVALRDDLSRRLDALYRMG
                     RLGYLQTLASADSGESFLRGLQLLSFLASRDARLLRDYEGSLVELAVKERNLTTLRTA
                     IAGVAAETREAERALQRARAEKATLLARLERTAEEQRSNVAALEDKTARLEALLELLE
                     TRGRALPAGSVSIRRYKGALDWPTRGKVVVPFGRIANPRFPRTFLRSSGWTIDAPTGT
                     PVRAVFAGDVVYAQWLKGYGNLVVLDHGDGVFTLYGHLMTGTVPRGTRVALGEVLGRV
                     ADPPEEEAAGVYFEIRESRASVDPRGWLR"
     gene            complement(12855..15029)
                     /locus_tag="PLP50_05565"
     CDS             complement(12855..15029)
                     /locus_tag="PLP50_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPA51053.1"
                     /translation="MPHLRMPPRPAAGLVLPAALLAVLAATAPAAPAEEVKGRVVVLG
                     FDGADHRLVSQMIAEGKLPNLAKLAREGSFSPLRPTIPAQTPVSWSTFSTGLSPGRTL
                     IFDFLKRDPKTYRPEFAIVSEGKKGFLLGRGNRAGAAAVGALLAFLLVFLIGRLVLKR
                     TKPAALVALGLALPAAFFSYRFGGLLPESLQTVVNHRQGTPFWEVAGKNGIRSVVLHV
                     PVTFPAVDYDEGRLLSGLGVPDLRGRIGTPSYYTSDPFFAPKNKNEFSVELIRLESNV
                     GTIRTEVFGPYNKLFPEPPVIKTPMTLTVLPDGGRLRIEPEGSDPVTIKPGEWSPWVT
                     FTFAFNPVVKLTGIGRFHLARIAPEVELYLSPIHFNPVDLPPNVKITAPRGLAKSLAK
                     RFGLYKTAGWSIDTWSMSEETIDEPTFLDDVTHTVQQYRKMMDGLLAEKDVRLYVQVY
                     EFTDRVGHVMWRFLDPTHPMYDAEKAAKFGPSVERNYRQMDEIVGDAQKELGPDDLLL
                     VCSDHGFASWRRSINYNTWLARNGYLGMKGGEEKLADLEQLFSQGEFWPNVDWSRTKA
                     YSMGLGEIYVNLRGREAQGTVEPGAEYDAVRSEIKTGLMALVDEATGRRPVERVYFRE
                     EAYPSFDANVIPDIFVTNSDGYRVGWQATLGVVTPELYEENRQVWSGDHCSVDPDVVP
                     GILFSSRPLRTTPRPGMADVPATIYKALGLTPPEPLDGAPLF"
     gene            15124..16449
                     /locus_tag="PLP50_05570"
     CDS             15124..16449
                     /locus_tag="PLP50_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA51054.1"
                     /translation="MSTGMLVALAVAAVAVIAAVVLFRSLSALKTASAEQSAQLEEKA
                     GQLEQEKERLAGQLAAAIEERDALAEKSAELGTRLAAVEAELDTTKAERDSLADKAKR
                     EQTTQRFFFGRKAREAFETFRQRLAADGFPERLEELAGDNPKTLTEEARKILKDVESW
                     AEQGGVEDATILHTLALVDYSRGDVERARLRLRKAARTSSDALLFENLGDLYRISGKL
                     RTALENYKIAAKTAPETSPVHRKLGLALYDAKDFGAAVKPLKTALELHREDPDLHLRT
                     ARALLESGDFQGTVEVAQAAGRKFPKDPLLPSCAILAYGRMKKFRDAQKTFEKAVEID
                     PKSADVHVAHGMALLDEGKLPEAAESFRTALECDASRPEAHYGLGLAANRDFAFDTAL
                     GHLKKSVGLKPDYAEAWYAMKTTYEGLKKFEAAVEALNKAIALNPRLSA"
     gene            complement(16519..17172)
                     /locus_tag="PLP50_05575"
     CDS             complement(16519..17172)
                     /locus_tag="PLP50_05575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPA51055.1"
                     /translation="MKIFLVRHGESEGNLGGTLQGCRLDTPLTRRGRRQAEALSVRLA
                     EAEIDDVVASPMARARETAAICAAPHGLAVRLDLELVEFDWGVWTGLPLDDEMDRSVA
                     ELRAKWRSGDVDAAPPRGESPIVAGARAARVLARLRAAGPAAPLVVAHGRFNRIFAAT
                     LLGRELARMDEVRQRNGSLSVFEWDGEGAARPLLLDDVSHLGGDVSTPTGKVDWVRA"
     gene            17208..18362
                     /locus_tag="PLP50_05580"
     CDS             17208..18362
                     /locus_tag="PLP50_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPA51056.1"
                     /translation="MSRTTEEQFPALSARIPDAEARAHFTWPFLRASEVFDELVDSAS
                     AEVLRRAGAWPVAPGTEPAALVAARGWAPRSLPLVAFVHRKLAAAGHLVASGGGYAPS
                     GTQPGDLAALEDELAAIDADSAVAGEAVRILVEEAGSFLSGEKTGEEILFRPDRLPLW
                     FRYFSNRNLLYGVNNSLGALALSRVLPASGARVLEIGGGAGSAAEAALAHLGGRVSRY
                     LFTEVVPTFARRGERAARAAAAPGVAVEAAKLDMTKPWAAQGAAPGAFDAVYSVNCFH
                     VAPDLSAVLAEAREALAPGGALVVSECVRPLGNDQPIYVELVFDLLESFTRVTLDPVA
                     RPTHGFLTPAAWRESLRRAGFVDVTFLPDAEELARSYPSFFASAVTARKG"
     gene            18364..18999
                     /locus_tag="PLP50_05585"
     CDS             18364..18999
                     /locus_tag="PLP50_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HPA51057.1"
                     /translation="MRLPRPLDAVEVRVLGALLEKELLTPDVYPMTLPGLLAACNQKT
                     SREPVTELSAAETTAALDRLRELVLVWKVTGGRAEKWEHNLEAKWQAGKPEKAILALL
                     LLRGAQTAAELRARAERMHELASVEAAEALLGAMAAHAEPLVAELPRRPGQKETRWTH
                     LASGSPPADASSPRAAAPPRTSPMEERLLTLEERVAALEAELAALRGSLGG"
     gene            complement(19011..19955)
                     /locus_tag="PLP50_05590"
     CDS             complement(19011..19955)
                     /locus_tag="PLP50_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51058.1"
                     /translation="MTIELRPLRESELREADGIYRRAFGTLRGLADPLTFDGDAERVR
                     CRWAADPDAAIALLEDGVLAGTSFVSRWGSVGLFGPLAVDPPRQGRGLAHPLVEEALR
                     LLDRPEVVLRGLVTSVSDPRTVGLYQRHGYWPVSLAVLMQKAPAAGRDAPAGFEALSR
                     LSPDAQAEALSACRELSDRVFDGLDVTVEILSVGEQETGDTVLLQDGPLLAGFAVCHA
                     GKGSEAGSGRAAIRFAAVPSGEGGEERLRRLLEAVEAWAAAAGATVVTAGTGSGRVGA
                     WNTLRAAGWLPCMQGAAMHRDRHPGYDREDVFVLDDWR"
     gene            complement(19997..22585)
                     /locus_tag="PLP50_05595"
     CDS             complement(19997..22585)
                     /locus_tag="PLP50_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPA51059.1"
                     /translation="MANERASELRCEAPRGWAETPARRATPEPLGRRLAEGGRLDLLR
                     TLRLAPRPGAPDGAAQVVLPGRRGEDLLVVLRQPSGALSIHRPVASTARRGAAEVVFE
                     LPLRRPPGRRGPAGDAVVLVLRAAARVEARAAATLARAFEETAFRRAGLAERLVHVTP
                     EGLRDGRLAPASPALLAPPPARNLLLLHGTFSSTASAYSDLARSGFFAALEPIYGGRV
                     VGFDHYTLSRTPAENARALAEALPGEEPFLFDVVAHSRGGLVLRTLLERPEEVGPVAS
                     RVATGRVVLAGVPNLGTPLASPGRWELALSGWLNLLDLLPKAPAPLLAAFVVEALLWL
                     ARRVTTLLPGLSAMDPAGETVAALGKRALPGAYSALAARHEATGALARRLLDAGADRF
                     FGEPNDLVVPTEGCIVLDRASGARVPAKSVALLGSGGSARPVPAHHFDLFSRDEAIAF
                     LAATLGGEPAAHVGAEESRRGRAPAIASTRAADAGAKPSRPARRAAAPRIGEARREAL
                     ELTLLPGDRRDLTALLFARYGTARVVVEVPRKGEAWREIIRGHERLRAHFDGDGRPLP
                     SESALERWGAVLFETLFPGDVRRLWDAARGAASGGATDVVFTSLLDWMADKPWELAFD
                     PHRRAFLAATEACLVRNVVTAVPCEARRRRRGRLSVVVAAAQPASLSSLSWEEEAEAV
                     RAGLAPLVSAGLARVRVERAVTAEALHRLVADGPCDVLHFVGHGTWDAGRRVGALVLE
                     SESGGVAPVEAPVLRTLLAGRGLALVVLNACQSARGSRGDLARGVAPALVAAGVPAVV
                     ANQFSVLDAAALVFARELYRSLALGRALGDAAREARLAVRCDAACGPVDWAVPVVYAR
                     DPGSTL"
     gene            22673..23617
                     /locus_tag="PLP50_05600"
     CDS             22673..23617
                     /locus_tag="PLP50_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51060.1"
                     /translation="MSRPPRIELPGALHHVSWRALERSPLFRDDVDRERFLADLGRTA
                     AERGWIVHAYALLGSSVEVLVETPAPNLSPGMRALGGRYAQAFNRRHGRGGPLFDGRF
                     RSLLIDPGTAFLDAVRVVALAPVRARLARAALDWRWSSAPSTAGLAERPAWLTVDATL
                     RRLSPARPRARERYRRLVSDTRNIRPLRERAVSRVVVGSAEFAREVRLRLAAPLPSSD
                     RPRPGAVELSRVRAAVASRFGVPEPALVRAGAHEAKVAAIWLSRRLTGLSGREVGEAF
                     GVLPARVSNVLGDVRTGRWRALRPMLEEMEKRLLAENE"
     gene            23610..25007
                     /locus_tag="PLP50_05605"
     CDS             23610..25007
                     /locus_tag="PLP50_05605"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPA51061.1"
                     /translation="MSERLLSVHKFGGTSLGDAERIVRAARLLAAASRRRRVVAVASA
                     MGGATDLLLRAAHEAERGRLGPALRSVAELRARHEAAAAGISAATGAAVGLDLAPFAE
                     LETALSGIALLRERTPRVTDLVASFGERLSVPLLAAALEATGVPARAVDARTFLVTDA
                     AHGAASCDRRKTDPRARRLLGRLLSGGIVPVVTGFVGRSPSGETTTLGRSGSDTTAAL
                     LGAALAAREVVIWTDVDGVLTADPRIVPEARLLPRLSYREAAEMTYFGAKVLHFPAVL
                     PAVAAKIPLVIRSSFAPEGPGTTISAKGDPRPGIRAVTAVSGLCLLSLDGKGMTGVPG
                     IAARVFQTTARLGVSVVMFSQASSEQNIAIVLPEADRARTAAALVQEFRYEKLVGAID
                     GVAARSPIAVVSAVGDGMRGTVGIAAKVFAAVARAGVNVESIAQGSSECNISFAVDEK
                     DRVAAVRALHEAVAP"
     gene            25004..25960
                     /locus_tag="PLP50_05610"
     CDS             25004..25960
                     /locus_tag="PLP50_05610"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPA51062.1"
                     /translation="MSAHVRAFAPATVANLGPGFDVLAAALDGPGDTVTAWRSKKPGV
                     RLVEVSGDRGRLPLDPERNTASVAAAFVLEAAVAAGRATPRTGVELALAKGLPLASGL
                     GSSAASAAAGAKAAALLLGERRSRVLLEAVLRGEHAADGSWHGDNGFAAILGGLVLVP
                     SSDPADYVPPVSLPVPPGLRFVLVHPDAELETKDARRVLPRELPLSEATRQAAALGAL
                     VDALHRGDLASAGAWMRSDRIAVPRRWALVPGWDEVTAAMKKAGALGWTLAGAGPTIL
                     ALSEEGSAPTKIAKAAERAFLALGVEADASVHAVSPRGAREA"
     gene            25961..27265
                     /gene="thrC"
                     /locus_tag="PLP50_05615"
     CDS             25961..27265
                     /gene="thrC"
                     /locus_tag="PLP50_05615"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPA51063.1"
                     /translation="MRAPLSRLSCLECGVTRAIEEVLTECPSCGGLLEVLHDLASFGR
                     SGREWRDLFDRRRSALPNAETPAFERSGVWRYREHVFPALAPEEIVSKPEGATRLYRC
                     VELGKELDLARLYVKHEGENPTLSFKDRGMTVGVSWARHLGRTDVVCASTGDTSAALA
                     AYAAEVPGMRAVVLLPEAKVTGEQLSQALHHGALTLGLATDFDGCMRIVADLARTRPV
                     YLLNSKNPVRIEGQKTIGQEAIHDLGWEVPDWFVVPVGNAGNVSAIGKGIREWLDLGV
                     IGKRPRIAGAQAEAADPFYRSFLTGFAERVTRTADETEASAIRIGAPVSHPRARREIE
                     LSNGTVTRVSEAELMEAAVLANRHGLAICPNSAVALAGAAKLRREGAIGRDETVVVVA
                     TAHAIKFSGAAVRYHGSGARHANPAQSLPASLEAVVAQLERC"
     gene            complement(27306..27980)
                     /locus_tag="PLP50_05620"
     CDS             complement(27306..27980)
                     /locus_tag="PLP50_05620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA51064.1"
                     /translation="MIIQCSACNTRYHYDEARFAGAPAKKIRCTKCSTVFEIRNPALD
                     APPPAGFQADETSSISAPMPGSDDFNFDTTVMGPHRKRPVVARPPEPIAAPAPPPRPP
                     APEVEKPPVAAAAAAGRRLRLPDWEKLSLACIAGPEAGRIFEIGKPRVVLGRAGVDIL
                     LNDPECSRSHAAIEISDDKVFLVDLGSTNGTYVADKRISQVELENRSEFDVGSSTLML
                     IRTRKD"
     gene            complement(28047..29213)
                     /locus_tag="PLP50_05625"
     CDS             complement(28047..29213)
                     /locus_tag="PLP50_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPA51065.1"
                     /translation="MTSLRTVYVDHHATTPLRPEALEAMRPWLTGAAANPSSLHAPGR
                     AARAAVERARERIAALLAVRPEELVFTSGGTESDALAVCGGARAVRDGEPLRTRVAVS
                     AAEHAAVREAARSLRREGFEPVEVPVDGMGLPGGQGLASAIDGRTALLSLILAGNETG
                     TLNGAIAESAATARGAGALVHTDAVQAVGKVPVRPSGLGVDLLTFTGHKLGGPRGAGV
                     LWVRRGVRLSPLFAGGGQERGRRGGTENVAAIVGLAAALDVVLPRLDEEGRRVGALRD
                     AFESGLRERVPGVRVNGLGPDGRLPTASSVTFPGVDGETLLVALDLEGVAASSGSACS
                     SGTPAPSRVLLACGLPEAEVRATLRFSFGWTTTEEDVVRLLEVLPPLVARVRAA"
     gene            29339..32443
                     /locus_tag="PLP50_05630"
     CDS             29339..32443
                     /locus_tag="PLP50_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA51066.1"
                     /translation="MVRTLARWLALVTAALAAPGAPAQAPPATPVPAPATAPADPSRG
                     VEVTGGAPDPAAVLRAEALTLFGRADDDRTAAWVASGGGPDARRVVSCPNLFGRIEVW
                     TYLSHRLLGPNARLLFFPEPGTGVGRYWTLLEGEPALLAAKSEATGPLGELPDGAFGC
                     ADAALVRDAVRSIRSRQGDTAGGLRERAALAAPISGAADAVPAASPAPLVFGGKRLSG
                     KEKKQALEALPERYQFFLRDVEPILTDVEETTFLRLGSDYQRDKFIEEFWKRRSVDSD
                     GQRVAFKDIYELRLRIVKERYRGVHTDQGRVFIVNGPPDGMRRIDCQDIYNPIELWYY
                     ERLESLRQSKVTLLFYQPFGTGDFRLWTPLDGQQAVLVGGVAGLAGISPGRRVDVTYC
                     QEWREVQGAMATAAGVFGTMGAQKMIDSLKSGPKPDVEGVDRILLMTTDLDPSAAKLP
                     VQRVFRFPEMVASKIRMEISLLLDRESLGTKALGEETFYDLDVVGEIVKGGKLVDNFR
                     YRFDFPASTVKGAFLPLGIERFLYPGEYRLKVKVADANRNAAALVDEKVVVPEAPDQV
                     LSPAEKAAREEAKAALARLVAEPNDRGVLSFLPLAREFATGVVRFETRASSDDIAYAE
                     FFLDGRKVVTDRRPPFEADFDLGELPRKRTVKVAGFAKDGRLLSEDEMILNEGREAFR
                     VRITSPEKGIQLEGPVRVVADLAVPQTKKLQQVEFWVNETRAATLYQPPFQQVVDVPR
                     QKEIGFLRVVATLADGSVSEDVRYFNAPKYLSEVDVQAVELYTSVFRKGRPVTGLSRE
                     SFRVLEDGAEQTIDGFEVVTNLPLALGIGVDTSGSMEETLPEAQKAANDFLRNVMTPR
                     DRCFLVTFDNEPQLVSGFTTDRDRLAQALAGLRAQGSTSLWDAVVYGLYQYQGTKGRK
                     AYVLLTDGSDRASKFTFEAALDYARKSGVAIYFIGLKIGGTQFDVRTKVNKMARETGG
                     TVYYVDSARALAGVYAEIDEELRSQYLLTYTPPARQGAKWRKVEVEVTPDDLEARTIS
                     GYYP"
     gene            32472..33047
                     /locus_tag="PLP50_05635"
     CDS             32472..33047
                     /locus_tag="PLP50_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPA51067.1"
                     /translation="MTRPPLLLASASPRRLELLRALGLEPLVCPADVDETLRPGEDPH
                     EAAERLARAKAAAVAQGAPTGAVVLAADTIVVLDGEALGKPRDRADARRMLHALRGRA
                     HDVVTGVALARDGRLVSGRETTEVLFAPMTDEEVDAYVASGEPSDKAGAYALQGLGGL
                     FVERITGTPSNVIGLPFRLVRRLGSEVGLQI"
     gene            complement(33227..35068)
                     /locus_tag="PLP50_05640"
     CDS             complement(33227..35068)
                     /locus_tag="PLP50_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HPA51068.1"
                     /translation="MTLGALGVVFGDIGTSPLYALRECFTGHHPIPPTPGNVLGILSL
                     IFWALVLTISVQYLAIVLRADNRGEGGILALMALLRPSGERGAGGGFLIVAVGLFGAA
                     LLYGDSIITPAITVLSSIEGLRIVTPAFDPYIRPIAVVVLLVLFSLQKKGTAGMGAIF
                     GPIMLVWFAVLAILGVVHVTTAPEVLSAVNPLHAVRFFQQNGVVGFLLLGAVFLVITG
                     GEALYADMGHFGAKPIRAGWYGLVLPSLLLNYFGQGALLLTKPDAAANPLFGMVPKWG
                     LLPLVALATIASVIASQAVISGAFSLTRQAIQLGYMPRLKIAHTSEKEIGQIYLPSIN
                     WILAIACVWLVLSFGSSSALASAYGIAVTTTMAITTTLMYVHARRAWNVPKAIALPVA
                     GLFLCIDLSFLGANLVKVFHGGWFPLAVATFVFMLMTTWKRGRQILSARLREASLPVD
                     VFLQDVEKRPPTRVRGTAVFMDRIPEGIPPPLLHNLKHNKILHERVVFLTVVTDEVPR
                     LKEKERSRLEYLGPGCWRVTLHYGFMENPDVPRALRKLKLDGKGFEPMETTFFLGRET
                     LIASKRPGMALWREHFFAWMSRSEGRATAFFNIPPGRVVELGLQVEL"
     gene            complement(35216..36175)
                     /locus_tag="PLP50_05645"
     CDS             complement(35216..36175)
                     /locus_tag="PLP50_05645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPA51069.1"
                     /translation="MPFSPASALSAGRLVTGSATFLLFALNTFFWCALLYLVTLLKLV
                     VPIAAFRRAASRLLVAIGEAWIAVNTVSLKLTQPTVYDVTGLEGLRRNVSYLVVSNHQ
                     SWVDIPVLQGVFLRRIPFLRFFLKQQLIRVPFLGAAWWALDFPFMKRHSREALERNPD
                     LRHEDLEATRRACERFRHAPAAILNFVEGTRFTPAKHARGGSPFRHLLVPKAGGLAFA
                     ASAMGPQLAGIVDVTIVYPHGSPTFWDLISRGLPEVVVRVQERAIPGGWFEGDYSDDA
                     AFRGSVQAWVRDLFLEKDAEIDALLASRREPAEPGRAPDGRLG"
     gene            complement(36212..39439)
                     /locus_tag="PLP50_05650"
     CDS             complement(36212..39439)
                     /locus_tag="PLP50_05650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA51070.1"
                     /translation="MRLRRLALLALGAVLAALPAAPRELGRLSLDEGLSQSIVEAIHQ
                     DRLGFLWLATEDGLNRWDGARFTVFRNVPGEARSFPYNDLKCLDEDGQGALWIGSFGG
                     GLNRFDPATGRVTAYRNDPADPTSLPADLVRAVRVDRDGRIWAGTQGGGLARLDPRTG
                     RFERFRHDARDGASLAHDDVRALLVDGRGTLWVGTYGGGLDRFDAASRRFVHVPSDGG
                     RGAELVDALLEDRSGHLWVGTWGGGLFLLDEESGRLVPRPRFARLPGERVKALAEDHE
                     AFLWIATDGAGLARYDRKTETVEVLSHDPSVPRSLATDRVFSLLEDRSRVLWIGTYGG
                     GLNTLDLGRKKFRNVRRDPHVPTSLGHDIVWAFHEDADGTVWVGTDSGGLQRWDRARD
                     VFRGFRHDPRDPASLAHDTVRALLGDAAGNLWVATNGGGLDRFDRATGRFAHHRHDPR
                     DPASLAHDELRTLYEDRSGTLWVGTYGGGLDRLERETGRFVHHRNDPADPATLSNDFV
                     RCVLEDSAGALWVGTQGGGLNRLDRATGRFTRFRANPADPASLSNDFVFALHEDRAGS
                     FWVGTYGGGLNRLDRAMGRFTRFTARDGLSSDSVYGILEDERGRLWISTNRGLTELDP
                     ADGTFRAWDETDGLQSDEFNGGAFYRSPRGEMFFGGIRGFDVFFPSEIAVRTEAAPVV
                     LTELLLANRPVPAGRPVDGRTLLPAFVTYADEVVLSHDDDVVSLEFAALHFAAPQKLR
                     YAFRLDGFDRDWIPTGADRRVATYTGLAPGRYLFRVKAANPDGVWGEREAHLKIVVTP
                     PFWGTWWFRLAAALGLAAAVVVALRRRMRTVRLAAAMRAAHDAQMAVMPHEDPEVEGF
                     EVSGVCIPALDVGGDFFDYVRSGEAGGKLGIVVGDVSGKGTPAAMAAAMSGGMVGALV
                     RRGEPLEAVAEQVNGALRAKIGRRMFAAACLATLDPERRELAFVNAGLCEPLLRSGDT
                     ATYLASEGSTFPLGAGARGRYRSRTVSLSPGDVVVLYTDGVPEAAGRAGTPWGYDALA
                     GFLRGLPTGSRTAREIRDAIVREVARVTGGSRPADDVAVVVVRAL"
     gene            39481..41661
                     /locus_tag="PLP50_05655"
     CDS             39481..41661
                     /locus_tag="PLP50_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HPA51071.1"
                     /translation="MRSRLDERAPDRHIRGLIRKRPPTRRTEAVDVLLVSMPFGPEMA
                     PSLGLSLLKAGLAPLGVSCRVLYLTLSFAERLGVGPYTRIATDGTRSIRELAGEHVFR
                     EALFGRDVEADARWERDVLLARACWPSKELARPVPPSFVGAVRRARSLAGPFLERALD
                     EVLAHRPRLVGFTSVFQQHVASLALARRIRTAAPETVVVIGGANCEGVMGAETARSFP
                     FLDAVVSGEGDLVFPELVARALAGRALDDLPGVATPGNVEAHFAAGRFPNAPSVTRMD
                     DLPVPDDGDYFEQFAQSRFAGTWTPGLFLETARGCSWGQKSHCTFCGLNGTTMAFRSK
                     SPARALAELRDVTSRHPESDLQVVDNILDTRYFESLLPELAKSPPPSPVFWETKASLK
                     KEQVRALAEAGVTRIQPGVESLSDAVLKLMKKGVTALQNVQLLKWCRELGVTPYWNLL
                     WGFPDEEPSEYARMARLVPLLAHLAPPAGVAGLRLDRFSPGFDDADRLGFLDVAPMPS
                     YAAVYPFPPDRLANLAYSFTFARRGPGEAAEYVAPLLRALARWKRVAGASALFSVPAG
                     DALFVFDLRTAARRPLTVLRGEARAVHDACDASTNVRAVVERVRSECGFAREATERAL
                     SALVRDRLLLEDGERRLALAVPLGRFAPDGRASARFHRLARRIGRRTRDGIAFPVHNA
                     AVTFRTRNGDDAPHAPRDTRTTSSSSEVLLDRFSVNRRGELVFS"
     gene            41699..41875
                     /locus_tag="PLP50_05660"
     CDS             41699..41875
                     /locus_tag="PLP50_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51072.1"
                     /translation="MATKKAAKKVAKKAVKKVAKKAPAKKAPAKKAVKKVAKKAVKKA
                     PAKKVAKKKDVGLI"
     gene            complement(41951..>42759)
                     /locus_tag="PLP50_05665"
     CDS             complement(41951..>42759)
                     /locus_tag="PLP50_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51073.1"
                     /translation="ADRDLSWLDATVVSDLSRDGRAILFAEQGAGGGAGTYSVYYRGT
                     EASAAARLGEGTAGPLSPDGKWAVAIVPSSPPSVVLLPTGPGRAKTLDRGPITSYQAA
                     AFFPGGERLLLAANEEGKPVRLYVQSTEAGGPRALPGEGLRFAPPSDVVSPDGRTAAV
                     SDGEGRVVFLPIDGAEPRLVPGLEPGDVPMRWSVDGGSLFVFRFDELPARIRRVELAT
                     RTREVVATIAPPDPAGVESIVAAQVTPDGRSWVYSFFQYRNDLHLVTGLK"
BASE COUNT         5917 a        15770 c        15177 g         5795 t
ORIGIN      
        1 ggcgcgaaac ccttgacact tgccgcgcgc gttcgcgccg cgaacgggcg acgcgaatcc
       61 agcgtttagt ccgcgcgggc ctcggtgatc tcttcggcta ttcgccttag gtcatcggcg
      121 acagagagaa catcagaata gagctgcctt agtctctcgg gagtcctgag ctccacggac
      181 agcggcatca agtgaccgtg catccccatc ttctcggtca tcgatgcgaa ctgcgcggcc
      241 atcgtgtcca tgttgcgagc aaggccgcgc agcgcttcgg cggcttccag gcgagcttct
      301 cgttcctccg gatcggtcat cacgattcct ccgcccttgt cgtcatcgga gtcgcggcat
      361 ctccggccag ctcgccttcg atttggtctt cgcgcctgtc aagtcccttg agccgctcgg
      421 tacttcgcga ggagccactg gtgatccttc tcgcacgaca gcaccccgtg aaagtcgctc
      481 gctgaaagac ctagcaccat ggcgttcgcc ttgatctcgc gctgcctcat ttctttcgca
      541 ccgtgcgaga tcttcaccca ccaagcagtc ttcttccctt cgaagtagaa gaaatagaac
      601 aggtgatctg cctgcttgcc ctcgaagccc ttcttcgaga gagaagacct gacggttcga
      661 acctcgatcg gcttcatccg aggaggtacg cgagcctcgc cttgattcgg gcggcatccg
      721 gcgcgagaag ctcaagaggt tcggaatcga gtgccgcctt cagcccacga atcgactctg
      781 acaggtcacg cagagcttcc tcatatgtgt ccccggtccc ggcgctggca aggaacggca
      841 tgaatgcaat gaatccttcg ctgtcgggct caacggtcac atcgaagcga acgggacacg
      901 aaagccggag agtcgcttcg tcggggcctc cgacgagttc ggtcagccag ctcacgatcg
      961 gcgcataccg gacggcatcc atggccgttt cgtcgggcat cgattcagca gaagacatcg
     1021 tccccagaag ccgcgagcgg cgtggagaga atgtctcgcc atggaggcga gtatagagag
     1081 cctcttcccc cgcgccggcc gtctgaattc gatctgccag gctcatccct ttccttccat
     1141 gaccctaagg gcctcgggct tgaccattga gaagaagagg gtctcgatcg tctgatgccc
     1201 ctcatcaagg aacgggtcaa tgtcaccgac cactgccggc ccgacgcgaa agaagtcgaa
     1261 gtcgagggcg aaccccgtga gtcgtacgcc cgccgggcca atcggcgtga agtcagaggg
     1321 ggccgcttca ccgaaagcaa gccggagagt ccctcccggc cgcttcagaa ggaatgccgc
     1381 ggcaacctcg ctcgcctccg actccccgag agcaggcagg cgcaagccga gctcaagcca
     1441 cggatgaagc gcttcagcag tgcactccat ggctgggaag atgttgacgt atctgaggcc
     1501 gagcctggtg agcgagtctg gacggtgaat ctcgaaaaac atggaagaca gccgccggaa
     1561 ttccgtcttg aattcttcga aaacgccgta gcgccttgac atgtaggaga agacgttgag
     1621 cgcgacgttc acagagcgcg agccgtcttc tgacttccac tgaaatggct tcaaggcgag
     1681 aggttcgcca gcggcagcct ttggaacgaa aagggagggg aactccggtc cgagcgctcg
     1741 ctggaagcgg tcgagtccgg cgtagacggc gaagttcccc gggaacctga tctcaaacac
     1801 gacctcgacg aggggtgcat tcgcaagccg agaactctct cgcattcctt ccaccgacta
     1861 cgtgccttcc tctccggtgg ggaccgcgtg tctcgcaggt cgaacccatc gcgattatgg
     1921 gccccgcttg cggaacgtgc acggcgggga ggatgaaatc ccgccccaaa cgcgtcccag
     1981 aggcacacgc gatccgggcg accggtaggc ggcctcccca ggttgggccc aagtgattga
     2041 aggggaagga gaaaagtggt gcgcccgccg cgattcgaac gcgggacctt tagatccgga
     2101 gtctaacgct ctatccgggc tgagctacgg gcgcgaattt cgaaagagcg gtgatgatgg
     2161 ggtgagagac gggggtcgaa cccgcgacca ccggaaccac aatccggcgc tctaccgact
     2221 gagctactct caccgcgctc attcggacgg cggaggatat cagaaacgcc ccgcgccgcc
     2281 atccccctcg gagtggcatc cttccgggcc atgacatctc ccgacggcgc aacgccttcc
     2341 gcctcgtccg agggctcgct ctcgaaccgg atcctgctcg ggctcctgct cggggcgatc
     2401 tgcggcgtcg gcgcgaacct cctcctcgtc ccgcgcttcc cggacgccgt ggaggccgcg
     2461 aaccggtggc tcttcgtccc ggtcggacag gtcttcctcc ggatgctctt catggtcgtc
     2521 atcccgctcg tcttcgcctc gctcgcgctc ggggtggcga gcctcggcga cctgaagcgg
     2581 ctcggccgcg tcggcggccg gacgatgctc tacttcctct ccgtcaccgc gttctccgtc
     2641 gccatcggcc tcgtcctcgt caacgtcctc aagcccggag agggcctccc ggcggagacc
     2701 cgcgagaagc tcctcgagac ctaccgcggc gaggcggcgt cccgcatgga gagcgcggag
     2761 aaggccgact tcgggccgtc gctcctcgtg aacatcgtcc cgcgcaaccc cgtcgccgcg
     2821 gcggcgaagg gcgacatgct cccggtgatc ttcttcgccc tcctcttcgg catcggcgtc
     2881 acgaagctgt cggccgagaa gaacgccctg tggatgaagc tcctcgaggg gctggccgac
     2941 gtcaccgtcg tgatcatcgg aatggcgctg aagctcgcgc cgttcggcgt cttcggcctg
     3001 atcttctccg tgacgtcgcg cttcggcttc gacatcctcc gccagctcct cttcttcgtc
     3061 gtcaccgtcc tcctggggct ctcgctcctg atcttcggcg tctacccgct ctacttgaag
     3121 ctccgtgcgg gcgtctcgcc gatcgccttc ctccggaaga tgcggccggt catgatcacg
     3181 gctttctcga cgagctcctc gagcgcgacg ctcccgacga cgatcgaggt gtcggagcgc
     3241 gagctcggcg tcccgccgca gatcgccggc ttcgtcctgc cgctcggcgc gacgatgaac
     3301 atgaacggga cggcgctctt cgaggggatc acctgcgtct tcctcgccca ggtcttcgga
     3361 gtccacctct cgctcggcca gcagctcttc gtcatcgtcc tcgccgtcct cacggcgatc
     3421 ggcgtcgcgg gtgtgccggg aggctccatc ccgctcctcg ttctcgtcct cacctccgtc
     3481 ggagtccccg ccgaggggat cgccgtcatc atcggagtcg accgcctcct cgacatggcc
     3541 cgcaccgtcc cgaacgtcgt cggcgacgcg agctgcgccg tctacgtcgc gaagaaggaa
     3601 ggcttcgagc tgaagatctg accatgacgt tcacgcacgc cctcgtgaga cctccggcct
     3661 cgaccttcgc cgacggcctg acgaccgccg gcctcggatc tcccgacctg ccgctcgccc
     3721 tcgcccagca cgaggcctac gtccgtgccc tcgaagcgtg cgggctctcc gtcacgcggc
     3781 tccctgccga cgaccgcttc ccggactcga cgttcgtcga ggacacggcc gtcctcgccc
     3841 gcggcctcgc cgtcctctgc cggccgggcg cgccgagccg ggccggcgag gtcgaggcga
     3901 tccgcaccgc cctcgagtcg ttcttcccct ctgccgtcgc catcgaggcg cccgggacgg
     3961 tcgacggcgg agacgtctgc gaggccggcg accacgtctt cgtcggcatc tcggagagga
     4021 cgaacgagac aggcgcccgc cagctcgccg cccacctcgc gacccgcggc ctcggaacga
     4081 ccctcgtcga cgttcgcggg atcccgggac tcctccacct caagagcgga atcgcgtggc
     4141 tcggcgagcg gaccctcgcg ctcgcgcgcc ccttcgccgg ccgccccgag ttcgcgggct
     4201 gggacgtcct cgacgtcccg gccggcgagg agtacgccgc gaacgccgtc ctcgtgaacg
     4261 gccacgtcct cgtcgccacg ggctacccgc gattcgcggc cgccgtgagc gcgctcggcc
     4321 tccccgtcct caccctcgac atgtcggagt tccgcaagct cgacggcggc ctgagctgcc
     4381 tctcgcttcg gttctagccg gtccgcccgc tgcgcggtcg ctccctcagg cggctggccc
     4441 gaagcccgca catcgcgatt cccgatctcg cggatcgctc gctccgacac ggacgtcgag
     4501 cggacgggag gggcgtcccg cgagcgagcg ttcgagcacg cgcggggtac ccgcacggct
     4561 ccgcgagcag gccgccgggg gacgagcgcg cacggattcg acgagcggag cgaggagctt
     4621 gcgcgctcgc cggcggcccg cgcagcggag ccgatgcggg tcgcgcgcgc gcagagctcg
     4681 ccgtgggacg ttcctcccgg ccgcgactcg tgagccacca cgagatggcc tggatggtct
     4741 ctccgtctct ccgtctctcc gtctctccgt ctctccgtct ctccgtctct ccgtctctcn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcttctctcc cccgtccccg
     4921 tgagagactc ccgcgccatg ccgggaaaga agagcgaggt gcgggcgtgg ctcgcttacg
     4981 actgggcgaa ctccgcgtgg gcgatgacgg tcgcggtggc cgtgctaccg gtctacttcg
     5041 cggggacggt cgtgccgaag gggggcgtcg agctcctcgg cttccggatc gacgcgacgg
     5101 tcctctgggg ctacgtgaac agcgcgtcga cgctcgcggt cttccttacg gcgccgatcc
     5161 tcggcgcgat cgccgaccgg ctcggcgcgc gccgcaagct cctcaccgcg ttcggtctcg
     5221 ccgggggcct cctcacgatg ctcctcgcgg cgtgcggccc gggcgacgtc gcgacgacgc
     5281 tcgtcattta cgccctctcg cagatcgcct tcgtctccgc gaacgtcttc tacgactcgt
     5341 tcctgccgca cctggcgaag gacgaggagc tcgaccgcct ctcggcgcgc ggctttgccg
     5401 tcgggtacgt cggaggctcg ctccacttcg tcctcgcgct catcctgatg gccacacacg
     5461 cgcgctgggg cctgacggcg gaagccgccg cacggatcgc tctcggctcc gccggcctct
     5521 ggtgggccgg cttcggggcg atcacgttcc tcggcctgag ggagccgccg cgggagggcg
     5581 agaggccggg cctcctccgc gcggtacgcg agggcatcgt cgacactctc gcgacgacgc
     5641 gccgcgtcgc gaaggtgaag ccggtcctcc tcttcctcct cgccttcgtc ctctacaacg
     5701 acggcgtcca gaccgtcatc aagatggcct cgctctacgg gaccgaggag gtcggcgtcg
     5761 ggttgccgat gatgatgggg acgctcctcg gcatccagct cctcgcgatg gtcggggctc
     5821 tcctcttcgc gcgcctcgcg gccgcctggg ggaccaagcg cgcgctcgtc ctcgccgtcg
     5881 cgctctggct cgtcgcggtc ggctgggcct acctcctgac gacgcccgcc ggcttcgtcg
     5941 tcctcgcggc gctcgcaggc ctcgtcctcg gggcggtcca ggcgctctcg cgctcgctct
     6001 tcgccgcgat gatcccgcgc gagtcgtcgg ccgagctctt cggcttcttc tccgtcttta
     6061 acaagctcgc gggcgttctc gggaccttcc tcttcgcgac ggtgcggcag gcgaccggga
     6121 cctcgcgcct cgcgatcctc tccgtcgccg tcctcttcgt cgccggcctc gtcctcctcc
     6181 tccgggtgaa cgaggccgag gcgcgcgcgc gccgcgacga gctgcgcggg gacggcgcta
     6241 caacaccgac caggcgaggg agttgaagag gaacgggaac gtcgcgttcg tctgcgcgcg
     6301 gtgctgcggg cggaacccga ggaggacgac cttccccttc ccgagcgtca gcgcgaccgc
     6361 ggcggcgttc cgggccagct tctcctcgcc gcggatccag ccggcgacga ggacgtcgcg
     6421 cccgtcggcc gggtaggccg cgagcacctc gcgcgtcatc tccgcccccg ggatcgtcgt
     6481 ctggaaggcg agcggcccgt ccaggaaccc gggaacggac gtgggaagcc cccacgtgac
     6541 cgggtgcgtc gggctcacgt cgagccggac gagcccgccg gggcagagga actcgtcgct
     6601 ctttacgcgc gcgagcgcgt tgcgcaccgg gagtccgagc tcctcgacga ggtactccgt
     6661 ggaggagccg agcgcgacga gcgtcccgcc cccttccacg aacgcccgga gcgccgccgc
     6721 gccctccttc ccgaggccgc ccgcgtactc cggccgcggc tcggggaagt agcgcatcgc
     6781 cccctcctcg cgcttcggct tcccgtccgc gatcacttcc ttgtcgacgt cggggaggac
     6841 gatcacgtcg aggcgcgcgg cgagcttccc ggcgcggagc gcggcgttgt cgagcgtcag
     6901 cggcgcgaag ccgtagcgct cgaggaggaa gcgcgtccac ccctcgtcca tcgaggcggt
     6961 ccacggcttg tagaggccga cgcgcggggc gcggaacggc ctcgcgccgg ccggcgcggc
     7021 cgcgagcggc gcgagcgaca ggccgaggtc cgcggcggcg cgcgaggccg cttcggcggc
     7081 ggccgcgtcg aggacgatcg tgccggcggc gagcgccccg tcggccgccg ggaggatcga
     7141 caccgcgccc cccttccgcg cggccgcgac gaccttccag cgctccgggc tctcggggcc
     7201 gagagcgacc gccgtcgtgc cggaggggag agatggcacc ggccccgtcg cgccgagagg
     7261 ccgcagggac tccgggagcc gccccttctc caccgtcacc cccatcgaga gcggcaggct
     7321 ccaggcggtg acgtcgtacg gcgccacgat ctccttcccc ggcacgagct tcacctcggg
     7381 gaaccgctgc acctcgagca tctcgcggac gaagcggccg tacggctgcg cgagcggcac
     7441 ccacgcatcg ccggtcgaat cggccttcac ctcgacgccg tgctcgacga ggagggcggc
     7501 cagcgcgcgc gccgtcgccg ggtcgcgctg ggccatcggg atgcggtacc cctcgccggg
     7561 cgcggcggcc gcgatcgagg cgcgcgcctt cgcgagcatg ttccggagga cgtcctcgcg
     7621 gtgcgtggcg cagtactcgt ggatcgcgtc ggaggcgacg cgctcgtagt ccatgacgtc
     7681 gcgcaggcgc cacgtcccgc cccgccaggg gttcgggaag ttgacctgcg ggccgtactc
     7741 gacgaggccc ttgccgcctc cgcggagctc ggtcggcggg acctcgatcg gcgtcgcgag
     7801 gcggaccgag gcgacctcgg tcaggacgcc cgtcacgttc ttccaccacg ccgtgttctt
     7861 cgagcccccc ggccaggaga cgtcgaacgc ccaggcggag atgacgccgc tcttcccggc
     7921 ctgctcgagc cgcatcgaca tcagcgaccc gatcatgttg acctcacgga tgacgagggg
     7981 gtcgatgtcg gggtcgagcg ggtcggcgta cggcggcacg aacatccgcg gcccgctcgt
     8041 ccccatctgg tgctcgtcga ggaagacctg cgggaaccag tcgtggtaga ggacgcggct
     8101 gatcgccttc gtctcggcct gcgtcagctg cgcgaagtcg cggttgttgt cgtgcccgac
     8161 gtagtggtga tagagccacg gcatccggct cccctcccag cgggtgccga ggttcttccg
     8221 gtaccagtcg acctccatcc ggtgcccgtc ggggttgagc gacgggacga ggaggaggac
     8281 gacctccgag aggcggcgct tcgtctcggc gtcctcggcc gttgcgagcg cgtgcgccca
     8341 ctccatcgcc atctgcgagg cgccgatctc gctcgcgtgg atgccgcagg tgacgaggag
     8401 gaccgccttc ccctcgccga cgagcgcggt cgcctccgcc tcggagaggc cgcgcgggtc
     8461 ggcgagacgg cgggcgacct cgcggagcct tcgctggttc gcgaggttct ccggcgagga
     8521 gatcgccgcg aggatcatct cctcgccgcg cgtcgaggtc ccgatcgtcg tcacctcgac
     8581 gcggtccgac gcggcgtcga gcgcgcggaa gtacgacacg atctggcccc agtcggccag
     8641 agtccggtcg gcgccgacgg ggagtctcag gtgctccgcc gggctcgggg gagcggcggc
     8701 ggccgggagc gcgagagaga ggacgaacgc ggcggcgagc gcggagcgga ggcggagggc
     8761 gcgcatgacc cgaaagtcta cgcgccgcgc ggcgttcaga acttcagctt gcgcgagacg
     8821 tcgaggacgg ccggggtgtt cgcgagcggg aggagagggg ccatcaggaa cgccttgacc
     8881 tcgctcgcca gctcctcgag cctcggttcg ccgacgccga aggtctcggc gaaccggccg
     8941 atgacggccc gctccgccgc cgagtagttc ccgtcgacga gggcgacgag gagggcggat
     9001 tgcagcagga gctcccggcc cttcggctcg gggagcgtct cggcgagctc cgccggggac
     9061 gggtcggagg ccgtggtgtc gacgggcgcg agctcctcga tgagggcgac ctcgcgcggg
     9121 tcggccgcgc cgtccgcgcg ggctacggcg agcatcgccc ggccgaccgc gttcgcctgc
     9181 gcgtcgttga gctccacgtc ctggaaaagg cgcatcgtcg ggccccccgt cgttctcgtt
     9241 ctgccgagtg gcggcggcga tgataccgct ccgggtcggg cccgaaaggg gcgccgcgcc
     9301 gccgccggcc ggcccgctca gtcgacgagc tcggagaccc ggacccgcag gacgccgctc
     9361 ttctcgagaa agcggaatcg gagctcgagg aagtcgacgg tcgcggcgtg ggggtgtccg
     9421 atgacgaccg cgtgcccctt cgtcgccgcc gtcgagaggg cgcgcgcgaa ggccgtcccg
     9481 agcgcctccg gagccccgcc gatcgccgcc gcctcggccc gcgcgtcgtc caggaagacg
     9541 tcgcgcggga tcgtcgcgag cccgagcgcc cgggcctctt ccgtcgcgac gctcgcggcg
     9601 gaggtcttcg agtcgaggaa gaagagcccc ttcgtccgga cgacggagag gacggcgcgg
     9661 acgaccctcc ggtcggccgt ggcgacggag ccctggtggt tgttcaggcc gatcgccccc
     9721 gggacggcgg cgatcgcctc ttccacgcgc cgggcgatcg tggcgtcgtc gtcgtccgag
     9781 gagatcgtcc ccggctcgta ggggccccgc gagcccgcca tggggagatg gacgaggagg
     9841 tcccatccct ttcgcttcgc gagggccgcc gcctcccgcg ccagtggcgc atcgggaaga
     9901 acggcgatcg cgagcggccc ggagagccgc tcgagacggg cgagcgaccc gtcggcgttc
     9961 ccgacgtcgt cgatgacgac ggcgatcgcg ccgcggcccg tgccggccgc cggctcgaag
    10021 tcgggcggga cgctcgtcgg ccggggaacc gccgtcgggg ccgggacagg ggtcggggcg
    10081 gcgatcgtcg ggaccggcgt cggggtcggc gtcggcggcg cctcgggcgt ccgtccgtag
    10141 cggagccaga ggaacgcgag ggcaaagagg aacgcgaggc ccgcgaggag cgccagcgcc
    10201 cgcccgcagc cgccccgccg ccgggccgcc gtcacgaggc gcgcgcgcgg ggagcgggga
    10261 gctcggcgac caccttcaga gcccgctcga ggaacgggtc cggaagcttc ccctcctccc
    10321 cttcctcggg gaggacgtgg aagacgcggt cgtcgggcgc gagacctctc ggcgagagcg
    10381 tccggccggc gggggtccgg actttcccga cggtgatccg gagcgcgccg ccgctcgcga
    10441 gacggacgac ctcctgggtc agacccatgc cgaaggtcgg ctccccgacg acgcggatcc
    10501 ggagctttgc cttgtcggga acggccaccg ccgcccccgg atcctccggc gcgaacgcct
    10561 cggccgactt ctcttcccgg cgcgctgccg cgccgacgtc gacgccgagc tcccgggccg
    10621 cgccctcgcg gagcgcggcg gcgaagagct cggcgggtcc gcccgtcccg ctgtcgacga
    10681 ggaggacgag ccccccgtcg tgcagacgcc ggcccgcttc gacggtgaag gtccgggtct
    10741 ggatcttccg tcccttcagc tcgccgatcg gcccgggcgg gccgagtagc gcggcggtcc
    10801 gggccgcttc ctcccaggtc ccccaggcgt ttccgcgcag gtcgaggacg agcgacttcg
    10861 tccggtcgag cggctcgagg atcttcgcga gggcgtcggc cgtcccgggc ccgaacgccg
    10921 gcaccttcac gacgagctcc ccggaaacgc gctcggccga gggaaggctc ggggtccagg
    10981 acgcgcgcac gatctcgagc gtgcgccgcc ggggcttctc ctcccggacg acgaggaccg
    11041 tccccttgcc cccgggcttg ccggagaggg cggcctcgac ctcccagaga gccaggcttc
    11101 gggccgagac gtcgcccacc ttctcgatca ggtcgtcggt cctgattccg gcggccgcgg
    11161 cggggctccc gggaacggcg gtgacgacga ccgggtagcc ggagcgtcgc gccagcacga
    11221 gccccgactc gacgtggtcg gtcttctcgc gggccgcgcg agccgcctcg aaggcggcca
    11281 tcttctcggg cgcgacgtac tccgagaacg ggtccatcgc ttcggtcatc cccgaaaagg
    11341 cccccgccag gagaggccgg acctcgacct tctcgacgta gttcccccgc acgagcgaaa
    11401 ggacctccgt gaagaggccg agaggctgat agagaccgtc tttcgcgccc ttccccttct
    11461 tctcgtcggc gcccagaccg gggaggacga cgaagagcgt gccgaggagg gcgagcgagc
    11521 cgaggaacga ggcgagccgg gacttcgtca ccgctcgatg atacgggatc gcgttcgggc
    11581 ggtttcgggg ccgtccacgc caggccgcct tcgccggccc gggttggcgg cgttctaccg
    11641 gagccagccg cgggggtcga ccgaggcgcg gctttcgcgg atctcgaagt agacgccggc
    11701 ggcctcctcc tcgggagggt ccgcgaccct cccgaggacc tcgccgagcg ccaccctcgt
    11761 gccgcggggc accgttcccg tcatcaggtg gccgtagagc gtgaagacgc cgtcaccgtg
    11821 gtcgaggacg acgaggttcc cgtatccctt gagccactgg gcgtagacga cgtcgcccgc
    11881 gaagacggcg cgcaccggcg tgccggtcgg cgcgtcgatc gtccacccgc tcgagcggag
    11941 gaaggtccgc gggaagcggg ggttcgcgat ccgcccgaag gggacgacca cctttccgcg
    12001 cgtcggccag tcgagagccc ccttgtacct ccgaatggag accgaaccgg ccgggagggc
    12061 ccggccccgc gtctcgagga gctcgaggag cgcctcgaga cgggccgtct tgtcctcgag
    12121 cgcggcgacg ttcgaccgct gctcctcggc cgtcctctcg aggcgcgcga ggagggtggc
    12181 cttctcggcg cgcgcgcgtt gcagagctct ctcggcctcg cgggtctcgg ccgcgacgcc
    12241 ggcgatcgcc gtgcggagcg tcgtgaggtt ccgctccttc acggcgagct cgacgagaga
    12301 gccctcgtag tccctgagga ggcgggcgtc ccgcgaggcc aggaacgaga ggagctggag
    12361 ccccctgaga aacgactcgc cggaatcggc ggaggcgagc gtctgaagat agccgagacg
    12421 acccatccgg tagagggcgt cgagcctccg ggagaggtcg tcccggaggg cgacgctctg
    12481 ccgtcgcgca tggtcccgct cggcctgcgc ctcggcggcg gcgcgttcgg tctccgcggc
    12541 gcgcagctcc aggatcctcc gctcggtggt ccgcagctcg aggttcgcct cggccgcctc
    12601 gagctgctgc cgcacgtcga cggcccgttg ccgggtctcc tcgtagcggg tcttcagccc
    12661 cgcgagccgg cggcggagcg cctccagctc ccggcgacgg tccgtcccgg ccgcgtcccg
    12721 cgtcacgacg gccggctctc gctcctgccc gtgggcgacg gaagaagcca gcagcgcgag
    12781 aaggggaaga aggcggagcc gcgagccgcg cctgcggcgg ttcccgcccc cggggcacgg
    12841 ctcaccgaac cgggtcagaa cagcggcgct ccgtcgagcg gctccggcgg cgtgaggccg
    12901 agagccttgt agatcgtcgc cggcacgtcg gccattccgg gtcgcggcgt cgtccggagc
    12961 gggcggctcg agaagaggat ccccgggacg acgtccgggt cgaccgagca gtggtccccc
    13021 gaccagacct gcctgttctc ctcgtagagc tcgggcgtca cgacgccgag cgtcgcctgc
    13081 cagccgacgc ggtacccgtc gctgttcgtc acgaagatgt cggggatgac gttcgcgtcg
    13141 aaggagggat aggcctcctc gcggaagtag accctctcga ccgggcggcg ccccgtcgcc
    13201 tcgtcgacca gggccatcag ccccgtcttg atctcgctcc ggaccgcgtc gtattccgcc
    13261 cccggctcga ccgtgccctg cgcctcgcgc ccccgcaggt tgacgtagat ctcgccgaga
    13321 cccatggagt aggccttcgt ccgggaccag tcgacgttcg gccagaactc cccctggctg
    13381 aagagctgct cgaggtcggc cagcttctcc tcgcccccct tcatcccgag gtagccgttc
    13441 cgggcgagcc aggtgttgta gttgatggag cggcgccagc tcgcaaagcc gtggtcggag
    13501 cagacgagga ggaggtcgtc ggggccgagc tccttctgcg cgtcgccgac gatctcgtcc
    13561 atctgcctgt agttccgttc gacggagggg ccgaacttcg ccgccttctc cgcgtcgtac
    13621 atcgggtggg tcgggtcgag gaagcgccac atcacgtgcc cgacccggtc ggtgaactcg
    13681 tagacctgga cgtagaggcg cacgtctttc tcggcgagga gcccgtccat catcttccgg
    13741 tactgctgga cggtgtgggt cacgtcgtcg aggaacgtcg gctcgtcgat cgtctcctcg
    13801 ctcatcgacc aggtgtcgat cgaccagccg gccgtcttgt agaggccgaa ccgcttggcc
    13861 aggctcttcg cgagcccccg cggggcggtg atcttcacgt tcggcgggag gtccaccggg
    13921 ttgaagtgga tcggcgagag gtagagctcg acctcgggag cgatccgcgc gaggtggaac
    13981 cggccgatac cggtgagctt gacgaccggg ttgaacgcga acgtgaacgt gacccagggg
    14041 gaccactccc ccggcttgat cgtcaccggg tcgctcccct cgggctcgat ccgcaggcgg
    14101 cccccgtcgg ggaggacggt cagcgtcatc ggcgtcttga tgacgggcgg ctcggggaag
    14161 agcttgttgt acggcccgaa gacctccgtc cggatcgtcc cgacgttcga ctcgaggcgg
    14221 atcagctcga cggagaactc gttcttgttc ttcggcgcga agaacgggtc ggaggtgtag
    14281 tagctcggcg tcccgatccg cccgcgcagg tccgggactc cgagacccga caggaggcgc
    14341 ccctcgtcgt agtcgacggc ggggaacgtc accgggacgt ggaggacgac ggaccggatc
    14401 ccgttcttcc ccgccacctc ccagaacggc gtcccctggc ggtggttgac gaccgtctgg
    14461 agcgactccg gcagcaggcc gccgaagcgg tacgagaaga aggcggccgg aagggcgagg
    14521 ccgagagcga ccagcgccgc gggcttcgtc cgcttcagga cgagccggcc gatgaggaag
    14581 acgaggagga acgccaggag cgctccgacc gccgccgcac cggcccggtt cccccggccg
    14641 aggaggaagc ccttcttccc ctccgagacg atcgcgaact cgggccggta ggtcttcgga
    14701 tcgcgcttca ggaagtcgaa gatcagcgtc cggccggggg agaggccggt cgagaaggtc
    14761 gaccaggaga cgggcgtctg ggccgggatc gtcggccgga ggggggagaa gctcccctcc
    14821 cgggcgagct tcgccaggtt cgggagcttc ccttcggcga tcatctggga gacgagccga
    14881 tggtcggcgc cgtcgaaccc gaggacgacg acccggccct tcacctcctc cgccggggcg
    14941 gccggagccg tggcggcgag gacggcgagg agggcggccg ggaggacgag accggcagcg
    15001 ggtcgggggg gcatccgaag gtgcggcacg cggcattcta atcacgcggt cgcgatccgc
    15061 ccccggccct cgggcggagg tagaattcgg ggtcgcgccg ttcccgttgc gcggaggagt
    15121 cccttgtcga ccggaatgct cgtcgccctc gccgtcgcgg ccgttgccgt gatcgccgcc
    15181 gtcgtcctct tccgctccct gtccgccctc aagaccgcct ccgcagagca gagcgctcag
    15241 ctcgaggaga aagccggcca gctcgagcag gagaaggagc ggctcgccgg gcagctggcc
    15301 gcggcgatcg aggaacggga cgccctcgcc gagaagtccg cggagctcgg cacccgcctc
    15361 gcggccgtgg aggccgagct ggacaccacg aaggccgagc gcgactcgct cgccgacaag
    15421 gcgaagcggg agcagacgac ccagcgcttc ttcttcggcc ggaaggcgcg ggaggcgttc
    15481 gagacgttcc gccagcgcct cgcggccgac ggcttcccgg agcgactgga ggagctggcc
    15541 ggcgacaacc cgaagacgct caccgaggag gcccggaaga tcctcaagga cgtcgagtcg
    15601 tgggccgagc aggggggcgt cgaggacgcc acgatcctcc acaccctcgc cctcgtcgac
    15661 tactcccgcg gcgacgtcga gcgcgcacgg ctccgcctgc gcaaggcggc gcggacctcg
    15721 tccgacgccc tcctcttcga gaacctgggc gacctctatc ggatctccgg gaagctccgt
    15781 accgcgctcg agaactacaa gatcgccgcg aagaccgccc cggagacctc cccggtccac
    15841 cgcaagctcg ggctcgcgct gtacgacgcg aaggacttcg gcgcggccgt caagccgctg
    15901 aagaccgccc tcgagctgca ccgggaggac ccggacctcc acctgaggac ggcgcgggcg
    15961 ctcctcgagt cgggcgactt ccaggggacc gtcgaggtcg cccaggccgc cggccggaag
    16021 ttcccgaagg acccgctcct cccctcctgc gccatcctcg cgtacggtcg gatgaagaag
    16081 ttccgcgacg cgcagaagac gttcgagaag gcggtcgaga tcgacccgaa gagcgccgac
    16141 gtccacgtcg cccacgggat ggccctcctc gacgagggga agctgccgga ggccgcggag
    16201 tcgttccgga ccgccctcga gtgcgacgcc tcccgtccgg aggcgcacta cggcctgggg
    16261 ctcgccgcca accgcgactt cgcgttcgac accgcgctcg ggcacctgaa gaagtccgtc
    16321 ggcctgaagc ccgactacgc cgaggcgtgg tacgcgatga agacgaccta cgaggggctg
    16381 aagaagttcg aggcggccgt cgaggccctg aacaaggcga tcgcgctcaa cccgcgcctg
    16441 tcggcgtgag tggatcgggg aggctcgcca cgagcctccc cgcggcgcct ccggcccctc
    16501 ccctcggtcg aggcggcgtc aggccctcac ccagtcgacc ttccccgtcg gcgtcgagac
    16561 gtccccgccg aggtgggaga cgtcgtcgag gaggagcggt cgcgcggccc cctccccgtc
    16621 ccactcgaag acggagagcg agccgttccg ctgacgcacc tcgtccatcc gggcgagttc
    16681 gcgcccgagg agcgtcgccg cgaagatccg gttgaagcgc ccgtgggcga cgacgagcgg
    16741 cgcggccggc ccggcggctc gcaggcgcgc gaggacgcgc gccgcacgcg cacccgcgac
    16801 gatcgggctc tcgccgcgcg gcggcgcggc gtcgacgtcc cccgaacgcc acttcgcgcg
    16861 gagctcggcg acgctccggt ccatctcgtc gtcgagcggc aggccggtcc agacgcccca
    16921 gtcgaactcc acgagctcga ggtccagcct caccgcgagg ccgtgcggtg ccgcgcagat
    16981 cgcggcggtc tcccgtgcgc gggccatcgg gctcgcgacg acgtcgtcga tctccgcctc
    17041 ggcgaggcgg accgagagag cctcggcctg gcggcggccg cgccgcgtga gcggggtgtc
    17101 gaggcggcac ccctggaggg tcccgccgag gttcccttcg ctctcgccgt gacggacgag
    17161 gaagatcttc atccgcgcga ttctacgaat ccgtatcatg cccgccgatg tcgaggacca
    17221 ccgaggagca gttcccggcg ctttccgccc ggattccgga cgccgaggcg cgcgcccact
    17281 tcacctggcc gttcctccgc gcgagcgagg tcttcgacga gctcgtcgac tccgcctcgg
    17341 ccgaggtcct ccggcgggcc ggcgcctggc cggtcgcccc cgggaccgag ccggcggcgc
    17401 tcgtcgccgc acgaggctgg gcgcctcgga gcctgccgct cgtcgccttc gtccaccgca
    17461 agctcgccgc cgcgggccac ctcgtcgctt ccgggggcgg gtacgcgccc tccggcaccc
    17521 agccgggcga cctcgcggcg ctcgaggacg agctcgccgc gatcgacgcg gactcggccg
    17581 tcgcgggcga ggcggtgagg atcctcgtcg aggaggcggg gagcttcctc tccggcgaga
    17641 agacgggaga ggagatcctc ttccgccccg accggctccc gctctggttc cgctacttct
    17701 cgaaccgcaa cctcctctac ggcgtcaaca actccctcgg cgccctcgcg ctctcgcgcg
    17761 tcctcccggc ctcgggcgcc cgcgtcctcg agatcggcgg cggcgcgggg agcgccgcgg
    17821 aggccgctct cgctcacctc ggcggacgcg tctcccgcta tctcttcacc gaggtcgtcc
    17881 cgacgttcgc gcggaggggc gagcgggccg cgcgcgccgc ggctgcgccg ggagtcgccg
    17941 tcgaggcggc gaagctcgac atgacgaagc cgtgggcggc gcagggagcg gcccccggag
    18001 cgttcgacgc cgtctactcc gtcaactgct tccacgtcgc ccccgacctc tcggccgtcc
    18061 tcgccgaggc gcgggaggcg ctcgcccccg gcggagcact cgtcgtctcc gagtgcgtcc
    18121 gcccgctcgg gaacgaccag ccgatctacg tcgagctcgt cttcgacctc ctcgagagct
    18181 tcacccgcgt cacgctcgac cccgtcgccc gcccgacgca cggcttcctg acgccggccg
    18241 cctggcgcga gtcgctccgg cgcgccggct tcgtagacgt gacgttcctc ccggacgccg
    18301 aggagctcgc gcggagctac ccgagcttct tcgcctcggc cgtgacggcg cggaagggct
    18361 gacgtgcgtc tcccccgccc gctcgacgcc gtggaggtcc gcgtcctcgg ggcgctcctc
    18421 gagaaggagc tcctgacccc cgacgtctac ccgatgacgc tcccggggct gctcgcggcc
    18481 tgcaaccaga agacctcgcg ggagccggtg accgagctct ccgccgcgga gacgaccgcc
    18541 gccctcgacc ggctccggga gctcgtcctc gtctggaagg tgacgggcgg gcgagccgag
    18601 aagtgggagc acaacctcga ggcgaagtgg caggccggga agccggagaa ggcgatcctc
    18661 gccctcctcc tcctccgcgg cgcgcagacc gccgccgagc tccgcgcccg cgccgagcgg
    18721 atgcacgagc tcgcctccgt cgaggccgcc gaggcgctcc tcggcgcgat ggccgcgcac
    18781 gccgagccgc tcgtcgccga gctcccgcgc cggccggggc agaaggagac gcgctggacc
    18841 cacctcgcct cgggctcgcc gcccgccgac gcgtcctcgc cgcgtgcggc cgcgccgccg
    18901 cggacgtcgc cgatggagga acgcctcctc accctggagg agcgcgtcgc cgcgctcgag
    18961 gccgagctcg ccgcgctccg gggctcgctc ggcgggtaga aggacgcagc ctagcgccag
    19021 tcgtcgagga cgaagacgtc ctcgcggtcg tagcccgggt gacggtcccg gtgcatcgcc
    19081 gccccctgca tgcaggggag ccagccggcc gcccggagcg tgttccacgc cccgacccgt
    19141 cccgagcccg tccccgcggt gacgacggtc gcgccggccg cggccgccca cgcctcgacc
    19201 gcctcgagga gacgccgcag gcgctcctcg cccccctcgc ccgaggggac cgcggcgaac
    19261 cgaatcgccg cgcgcccgga gcccgcctcg ctccccttcc cggcgtggca gacggcgaag
    19321 cccgccagga gcggcccgtc ctggaggagg accgtgtcgc ccgtctcctg ctcgccgacg
    19381 gagaggatct cgaccgtcac gtcgaggccg tcgaagaccc ggtccgagag ctcccggcag
    19441 gccgagagcg cctccgcctg agcgtcgggg gagaggcgcg agagcgcctc gaagccggca
    19501 ggcgcgtccc gtccggccgc cggcgccttc tgcatcagga cggccaggct caccggccag
    19561 tagccgtggc gctggtagag gccgaccgtc ctcgggtccg agacggaggt gacgaggccc
    19621 cggagcacga cctcgggccg gtcgaggagc cggagcgcct cctcgacgag cgggtgcgcg
    19681 aggccgcgcc cctggcgcgg cgggtcgacc gcgaggggtc cgaagaggcc gacgctcccc
    19741 cagcgggaga cgaagctcgt cccggcgagc acaccgtcct cgaggagggc gatcgcggcg
    19801 tccgggtcgg cggcccaacg gcaccgcacg cgttcggcgt cgccatcgaa ggtgagaggg
    19861 tcagccaagc cccggagcgt cccgaaggct cgccggtaga tcccgtcggc ctcgcgcagc
    19921 tcgctctcgc gaagcggtcg gagctcgatc gtcatggcgc gacctccttc tccatcttgc
    19981 accgcctccc ggcctttcag agcgtcgagc ccggatcgcg cgcgtagacg accgggacgg
    20041 cccagtcgac cgggccgcac gcggcatcac aacggacggc caggcgggcc tcgcgcgccg
    20101 cgtcgcccag ggcgcgcccg agcgcgagcg acctgtagag ctcgcgcgcg aagacgagcg
    20161 ccgcggcgtc gaggacgctg aactggttcg cgacgacggc ggggactccg gcggccacga
    20221 gcgccggggc gacgccgcgg gccaggtcgc cccggctccc ccgcgcgctc tggcaggcgt
    20281 tcaggacgac gagggcgagc ccccggccgg ccaggagcgt cctgaggacg ggcgcttcga
    20341 cgggcgcgac ccctccgctc tccgactcga ggacgagcgc gccgacccgg cgcccggcgt
    20401 cccacgtgcc gtggccgacg aagtggagga cgtcgcaagg gccgtcggcc acgagccggt
    20461 ggagcgcttc ggcggtgacg gcccgctcca cccggacccg cgcgagcccg gccgagacga
    20521 gcggcgcgag gccggcgcga accgcctccg cctcctcctc ccacgacagg gacgagaggc
    20581 tcgccggctg cgccgcggcg acgacgaccg agaggcggcc cctcctccgc cgtcgtgcct
    20641 cgcaggggac ggccgtgacg acgttccgca cgaggcaggc ctcggtcgcg gcgaggaacg
    20701 cccggcggtg cgggtcgaac gccagctccc acggcttgtc ggccatccag tcgaggagcg
    20761 acgtgaagac gacgtccgtc gccccgcccg aggcggcccc gcgcgccgcg tcccagagtc
    20821 tccgcacgtc ccccgggaag agcgtctcga agaggaccgc gccccatcgc tcgagcgcgg
    20881 actccgacgg gagcgggcgc ccgtcgccgt cgaagtgggc gcggaggcgc tcgtggccgc
    20941 ggatgatctc ccgccaggcc tctccctttc gcggcacctc gacgacgacc cgggccgtgc
    21001 cataccgagc gaaaaggagc gccgtgaggt cacgccggtc gcccggcagg agcgtcagct
    21061 cgagcgcctc gcgccgcgcc tcgccgatcc ggggagccgc ggcccggcgt gccggccggg
    21121 agggcttcgc ccccgcgtcg gcagcgcggg tggacgcgat cgcgggagcc cggccgcggc
    21181 ggctctcctc cgctccgaca tgggctgccg gctcgccccc gagcgtcgcg gcgaggaacg
    21241 cgatcgcctc gtcgcgagag aagaggtcga agtggtgcgc gggcacgggc cgcgcggacc
    21301 cgcccgaccc gaggagcgcg acgctcttcg ccgggacgcg ggcgccgctc gcccggtcga
    21361 ggacgatgca cccctcggtc gggacgacga ggtcgttcgg ctcgccgaag aaccggtctg
    21421 cccccgcgtc gaggaggcgg cgcgcgagcg cccccgtcgc ctcgtgccgc gcggcgaggg
    21481 ccgagtaggc tcctggcagc gcgcgcttcc cgagggcggc gaccgtctcg cccgccgggt
    21541 ccatcgccga gaggcccgga aggagcgtcg tcacgcgccg cgcgagccag aggagcgcct
    21601 cgacgacgaa ggccgcgagg agaggcgcgg gcgccttcgg caggaggtcg aggaggttca
    21661 gccagcccga gagggcgagc tcccagcgcc cgggcgaagc cagcggcgtc ccgaggttcg
    21721 gcacgcccgc gaggaccaca cgccccgtgg cgacgcggct cgcgacgggg ccaacctcct
    21781 cgggcctctc cagcagcgtc cgcaggacga ggccgccgcg ggagtgggcc acgacgtcga
    21841 agaggaacgg ctcctccccg ggcagcgcct ccgcgagcgc ccgcgcgttc tcggccggcg
    21901 tccggctgag cgtgtagtgg tcgaagccga cgacgcgccc tccgtagatc ggctcgagcg
    21961 cggcgaagaa cccgctgcgg gcgaggtcgg agtaggccga cgccgtgctc gagaacgtgc
    22021 catggaggag gaggaggttc cgggccggcg gcggggcgag gagcgccgga gaggcgggcg
    22081 cgaggcggcc gtctcgaagc ccctccggcg tcacgtggac gagccgttcg gccagtcccg
    22141 cgcgacggaa ggccgtctcc tcgaacgccc gggcgagcgt cgcggcggcc cgcgcctcga
    22201 cgcgcgccgc cgcgcggaga acgaggacga ccgcgtcgcc ggccggaccc ctccgcccgg
    22261 gcggccgtcg gaggggcagc tcgaagacga cctcggccgc gccgcgacgc gcggtggagg
    22321 cgacgggacg gtggatcgag agcgcgcccg acggctgccg gaggacgacg aggaggtcct
    22381 ctccgcggcg ccccggcagg acgacctgcg ccgccccgtc cggggctccc ggccggggcg
    22441 ccagccgaag cgtccggagg aggtcgaggc gcccgccctc cgccagccgg cggccgagcg
    22501 gctcgggcgt cgcgcgacgc gccggcgtct ccgcccaccc gcgcggcgcc tcgcaccgca
    22561 gctcgctcgc gcgctcattc gccatcggcc tctctcccgg aggcggcatc gtaccgccgc
    22621 cgatgcgcct gcgccgcgga ggtggggccg cccgcctcta caatcgaggc acatgtcgcg
    22681 tccgccccgc atcgagctcc cgggagccct gcaccacgtc tcctggcggg cgctcgagcg
    22741 gagcccgctc ttccgggacg acgtcgaccg ggagcgcttc ctcgccgacc tcgggcggac
    22801 cgcggcggag cggggctgga tcgtccacgc ctacgcgctc ctcgggagct ccgtcgaggt
    22861 cctcgtggag acgccggcgc cgaacctctc gcccgggatg cgtgccctcg gcggccgcta
    22921 cgcgcaggcc ttcaaccggc gccacggccg gggcgggccg ctcttcgacg gccgcttccg
    22981 ctcgctcctg atcgaccccg gcaccgcctt cctcgacgcc gtccgcgtcg tcgccctcgc
    23041 ccccgtgagg gcccgcctcg cgcgggcggc gctcgactgg cgctggtcga gcgcgccctc
    23101 cacggccggc ctcgccgagc gcccggcatg gctcaccgtc gacgccacgc ttcgtcgtct
    23161 ttccccggcc cggccgcgcg cccgcgagcg ctaccggcgg ctcgtctccg acacgcggaa
    23221 catccggccg cttcgcgagc gcgccgtctc gcgggtcgtc gtcgggagcg ccgagttcgc
    23281 gcgcgaggtc cggctccgcc tcgccgcgcc gctcccgtcg tccgatcggc cgcgtcccgg
    23341 cgccgtcgag ctctcgcgcg tgagggccgc cgtcgcctcg cggttcggcg tccccgagcc
    23401 ggcgctcgtc cgggccggcg cgcacgaggc gaaggtggcc gcgatctggc tctcgcgccg
    23461 cctcacgggc ctctccgggc gcgaggtggg agaggccttc ggcgtccttc ccgcgcgggt
    23521 cagcaacgtc ctcggcgacg tccggacggg gcgctggagg gcgctccggc cgatgctcga
    23581 ggagatggag aagaggctcc tcgccgagaa tgagtgaacg cctcctctcc gtccacaagt
    23641 tcggcggcac ctcgctcggc gacgcggagc ggatcgtccg ggccgcgcgc ctcctcgccg
    23701 cggcgtcccg ccgccgccgt gtcgtcgccg tcgcctccgc gatgggaggc gccaccgacc
    23761 tcctcctccg cgccgcgcac gaggcggaac gggggcggct cggtccggcg ctccggagcg
    23821 tcgccgagct gagggcccgg cacgaggccg ccgccgccgg gatctccgcg gcgaccggcg
    23881 cggccgtcgg cctcgacctc gcccccttcg ccgagctcga gacggccctc tcggggatcg
    23941 cgctcctgcg cgagcggacg ccgcgcgtca ccgacctcgt cgcctccttc ggcgagcgcc
    24001 tctcggtgcc gctgctcgcc gccgccctcg aggcgacggg cgtccccgcc cgcgccgtcg
    24061 acgcccggac gttcctcgtg accgacgcgg cgcacggcgc cgcgtcatgc gatcgccgga
    24121 agaccgaccc gcgggcgcgg cgcctcctcg gccgcctcct ctccggggga atcgtcccgg
    24181 tcgtcaccgg cttcgtcggg cggtcgcctt cgggcgagac gacgacgctc ggccgctccg
    24241 gctccgacac gacggccgcc ctcctcggcg cggctctcgc ggcgcgcgag gtcgtcatct
    24301 ggaccgacgt cgacggcgtc ctgacggccg acccccggat cgtccccgag gcccgtcttc
    24361 tgccgcgcct ctcctaccgc gaggcggcgg agatgaccta cttcggcgcg aaggtgctcc
    24421 acttcccggc cgttctcccg gccgtcgcgg cgaagatccc gctcgtcatc cggagctcct
    24481 tcgcgccgga ggggccgggg acgacgatct cggcgaaggg ggacccgcgc cccggcatcc
    24541 gcgccgtcac ggccgtctcc ggcctctgtc tcctctcgct cgacgggaag gggatgacgg
    24601 gcgtgccggg gatcgcggcc cgcgtcttcc agacgacggc ccggctcggc gtctccgtcg
    24661 tcatgttctc ccaggcgtcg tccgagcaga acatcgccat cgtcctgccc gaggccgacc
    24721 gcgcacggac cgcggcggct ctcgtgcagg aattccgata cgagaagctc gtcggcgcga
    24781 tcgacggcgt ggcggcgcgg tcgccgatcg ccgtcgtctc cgccgtcggg gacgggatgc
    24841 gcggcaccgt cggcatcgcg gcgaaggtct tcgccgccgt cgcgcgcgcg ggagtgaacg
    24901 tggaatcgat cgcccagggc tcctcggagt gcaacatctc cttcgccgtc gacgagaagg
    24961 accgcgtggc cgccgtgcgc gcgctccacg aggcggtggc gccgtgagcg cgcacgtccg
    25021 cgcgttcgcc cccgcgaccg tcgcgaacct cgggccgggg ttcgacgtcc tcgcggcggc
    25081 gctcgacggc cccggagaca cggttaccgc ctggcgctcg aagaagccgg gcgtgcggct
    25141 cgtggaggtc tccggcgacc gggggcgcct ccccctcgac ccggagcgga acacggcctc
    25201 cgtcgcggcg gcgttcgtcc tcgaggccgc ggtcgccgcc ggccgcgcga cgccgcgcac
    25261 gggggtcgag ctcgcgctcg ccaaggggct ccctctcgcg agcggcctcg gctcctccgc
    25321 cgcgtccgcg gccgccgggg cgaaggccgc ggccctcctc ctcggcgagc ggcgctcacg
    25381 cgtcctgctc gaggcggtcc tccgcggcga gcacgccgcc gacgggtcct ggcacggaga
    25441 caacggcttc gccgcgatcc tcggcggcct cgtcctcgtc ccctcttccg acccggccga
    25501 ctacgtcccg ccggtctccc ttccggtccc gcccgggctc cgcttcgtcc tcgtccaccc
    25561 cgacgccgag ctggaaacga aggacgcgcg ccgggtcctc ccgcgcgagc tcccgctctc
    25621 cgaggcgacc cgccaggccg cggccctcgg cgcgctcgtc gacgccctcc accgcggcga
    25681 cctcgcctcg gccggagcct ggatgcggtc cgaccggatc gccgtccccc ggaggtgggc
    25741 cctcgtgccg ggatgggacg aggtgacggc cgcgatgaag aaggccggcg ccctcggctg
    25801 gacgctcgcc ggcgcgggcc cgacgatcct cgccctctcg gaggagggga gcgcgccgac
    25861 gaaaatcgcg aaggcggccg agagggcctt cctcgcgctc ggcgtggagg ccgatgcgag
    25921 cgtccacgcc gtctcgccgc ggggggcgcg ggaggcgtag ttgcgagcgc ccctctcccg
    25981 cctctcctgc ctcgagtgcg gcgtgacccg cgcgatcgag gaggtgctca ccgagtgccc
    26041 ctcgtgcggc ggcctcctcg aggtcctcca cgacctcgcc tcgttcggga gatcgggccg
    26101 cgagtggcgc gacctcttcg atcgccggcg ctccgcgctg ccgaacgcgg agacgcccgc
    26161 gttcgagagg agcggcgtct ggcgctaccg cgagcacgtc ttcccggcgc tggcgccgga
    26221 ggagatcgtc agcaagcccg agggcgcgac ccgcctctac cgctgcgtcg agctcgggaa
    26281 ggagctggac ctcgcccgcc tgtacgtcaa gcacgagggg gagaacccga cgctctcctt
    26341 caaggaccgc ggcatgaccg tcggcgtttc ctgggcgcgg cacctcggcc ggaccgacgt
    26401 cgtctgcgcc tcgacgggcg acacctccgc cgcgctcgcc gcttacgccg ccgaggtgcc
    26461 ggggatgcgc gccgtcgtcc tcctcccgga ggcgaaggtc acgggcgagc agctctcgca
    26521 ggcgctccac cacggcgcgc tcacgctcgg cctcgcgacc gacttcgacg gctgcatgcg
    26581 gatcgtcgcc gacctcgcgc ggacgcgccc cgtctacctc ctgaactcga agaacccggt
    26641 gcggatcgag gggcagaaga cgatcgggca ggaggcgatc cacgacctcg gctgggaggt
    26701 ccccgactgg ttcgtcgtcc cggtcggcaa cgcggggaac gtctcggcga tcggcaaggg
    26761 gatccgcgag tggctcgacc tcggcgtcat cgggaagcgc ccgcgcatcg ccggagcgca
    26821 ggccgaggcg gccgacccgt tctaccgctc cttcctcacc ggcttcgccg agcgcgtcac
    26881 gcgcaccgcg gacgagaccg aggcctcggc gatccggatc ggagccccgg tctcgcaccc
    26941 gagggcacga cgcgagatcg agctctcgaa cggcacggtc acgcgcgtct ccgaggccga
    27001 gctgatggag gcggcggtgc tcgcgaaccg gcacggtctc gccatctgcc cgaactccgc
    27061 cgtcgccctc gccggtgcgg cgaagctccg ccgggaaggc gcgatcgggc gggacgagac
    27121 cgtcgtcgtc gtcgcgacgg cccacgccat caagttctcg ggggccgccg tgaggtacca
    27181 cgggagcggc gcgcgccacg cgaacccggc ccagagcctc cccgcctccc tcgaggcggt
    27241 cgtcgcgcag ctggagcgct gctgaagggg cgcggggcgc cccgcgctcc ggagagagcg
    27301 ggaggctagt ccttccgcgt ccggatgagc atcagcgtcg aggagccgac gtcgaactcc
    27361 gaccggttct cgagctcgac ctgcgagatc cgcttgtcgg cgacgtaggt cccgttcgtc
    27421 gagccgaggt cgacgaggaa gaccttgtca tcggagatct cgatcgcggc atggctgcgc
    27481 gagcactcgg ggtcgttcag gaggatgtcg acgccagcgc gaccgagaac gacgcgcggc
    27541 tttccgatct cgaagatccg ccccgcctcc ggtcccgcga tgcaggcgag cgacagcttc
    27601 tcccagtccg gcaggcggag gcgtcggccg gccgcggccg cggccgccac cgggggcttc
    27661 tccacctcgg gcgcaggcgg acggggaggc ggagccggag cggcgatcgg ctcgggtggc
    27721 cgggcgacga ccggccgctt ccggtgagga cccatcacgg tcgtgtcgaa gttgaagtcg
    27781 tcggagccgg gcatcggcgc gctgatcgag ctcgtctcgt cggcctggaa ccccgccggc
    27841 ggcggcgcgt ccagggcggg gttccggatc tcgaagacgg tcgagcactt ggtgcagcgg
    27901 atcttcttcg cgggggcgcc tgcgaagcgg gcctcgtcgt agtggtagcg cgtgttgcag
    27961 gcagagcact ggatgatcaa cgcatctccc ccacggtcga tccggattga gaacctgcgc
    28021 cattagacac gcccgcgccc cccggatcaa gccgcccgaa cgcgcgcgac gaggggcgga
    28081 aggacctcca ggagccgcac gacgtcttcc tccgtcgtcg tccacccgaa cgagaaacgc
    28141 agggtcgccc ggacctcggc ctccggcagg ccgcaggcga ggaggacgcg gctcggcgcc
    28201 ggcgtccccg acgagcaggc cgagccggac gaggcggcga caccttcgag gtcgagagcg
    28261 accagaagcg tctcgccgtc gaccccggga aacgtcaccg agctcgccgt cggaagccgc
    28321 ccgtccggcc cgaggccgtt gaccctcacc ccgggcaccc tttcccggag ccccgactcg
    28381 aaggcgtccc ggagcgcgcc gaccctccgg ccctcctcgt cgaggcgggg aaggaccacg
    28441 tccagcgccg cggcgagccc cacgatggcg gcgacgttct cggtcccgcc tcgccggccc
    28501 cgctcctgcc cgccgcccgc gaagagcggg gacagtcgaa cgcctcgcct cacccagagg
    28561 acgccggccc cccggggccc gccgagcttg tggcccgtga aggtgagcag gtcgacgccg
    28621 agcccggagg ggcggacggg gaccttcccg accgcctgga cggcgtcggt gtggacgagc
    28681 gcacccgcgc ctcgggcggt ggcggccgat tccgcgatcg cgccattcag ggtccccgtc
    28741 tcgttcccgg cgaggatcag agagaggagg gccgtccggc cgtcgatcgc ggaagcgagg
    28801 ccctgaccgc ctggcagccc cataccgtcg acggggacct cgaccggctc gaacccctcg
    28861 cgtcgcaggg agcgcgccgc ctccctcacc gccgcgtgct cggccgccga gacggcgacg
    28921 cgggtccgaa gcggctcgcc gtcccgcacc gcacgcgccc cgccacagac cgcgagggcg
    28981 tccgactccg tcccgcccga ggtgaacacg agctcctcgg gacggacggc gagcagcgcc
    29041 gcgatcctct ccctcgcccg ctcgacggcc gcccgggccg cacgccccgg cgcgtggagc
    29101 gaggaggggt tcgccgcggc gccggtcagc cagggacgca tcgcctcgag ggcctccggc
    29161 cggagcgggg tcgtggcgtg gtggtcgacg tagaccgtcc ggagggaggt catcggcccg
    29221 gattctagga gcgcgcgtct tcgccgccgc aaaccccggt ccccgtcgga gccgtacact
    29281 ccggcacggt ccctcccttg cttgccgcga aggaggggac gccaaggagg cggaactgat
    29341 ggtccggacg ctcgcacgat ggctcgccct cgtgaccgcc gcgctcgccg ctcccggcgc
    29401 gccggcccag gccccgcccg cgacgcccgt tccggcgccg gcgaccgccc cggcggaccc
    29461 gtcgcgcggg gtcgaggtca ccggcggcgc ccccgatccc gccgccgtcc tgcgcgccga
    29521 ggcgctcacc ctcttcggcc gcgccgacga cgaccgaacg gcggcatggg tcgcctccgg
    29581 gggcggtccc gacgcgcggc gggtcgtctc ctgcccgaac ctcttcggcc ggatcgaggt
    29641 ctggacctat ctctcgcacc gtctcctcgg gccgaacgcc cggctcctct tcttcccgga
    29701 gccggggacc ggggtcggcc gctactggac tctcctcgag ggcgagccgg cgctcctcgc
    29761 ggcgaagtcg gaggccaccg gaccgctcgg cgagctcccg gacggcgcct tcgggtgcgc
    29821 cgacgcggcg ctcgtgcgcg acgccgtccg ctccatccgg tcgcgccagg gcgacaccgc
    29881 cggcggcctg cgggagcgcg cggcgctcgc cgccccgatc tccggcgcgg cggacgcggt
    29941 tccggcggcc agcccggcgc cgctcgtctt cggcggcaag cgcctttccg gcaaggagaa
    30001 gaagcaggct ctcgaggcgc tgcccgagcg ctaccagttc ttcctccgcg acgtcgagcc
    30061 gatcctgacg gacgtcgagg agacgacgtt cctgcgcctc ggctccgact accagcgcga
    30121 caagttcatc gaggagttct ggaagcggcg ttccgtcgac tccgacggcc agcgtgtggc
    30181 gttcaaggac atctacgagc tgcgactccg gatcgtcaag gagaggtacc gcggcgtcca
    30241 cacggaccag gggcgagtct tcatcgtcaa cggcccgccc gacgggatgc gccggatcga
    30301 ctgccaggac atctacaacc cgatcgagct ctggtactac gagcggctcg agtcgctccg
    30361 gcagagcaag gtcacgctcc tcttctacca gccgttcggc accggcgact tccgcctctg
    30421 gacgcctctc gacggacagc aggcggtgct ggtcggggga gtggccggcc tggccggaat
    30481 ctcgcccggc cggcgggtcg acgtcaccta ctgccaggag tggcgcgagg tccagggcgc
    30541 catggcgacg gccgcaggcg tcttcgggac gatgggcgcg cagaagatga tcgactccct
    30601 gaagtcgggg ccgaagccgg acgtggaagg ggtcgaccgg atcctcctga tgacgaccga
    30661 cctcgacccg tccgcggcga agctcccggt ccagcgggtc ttccggttcc ccgagatggt
    30721 cgcgagcaag atccggatgg agatctcgct cctcctcgat cgcgaatcgc tcggcacgaa
    30781 ggccctcggc gaggagacgt tctacgacct cgacgtcgtc ggcgagatcg tcaagggagg
    30841 gaagctcgtc gacaatttcc gataccgttt cgacttcccc gcctcgacgg tgaagggggc
    30901 gttcctcccg ctgggcatcg agcggttcct ctacccgggc gagtaccggc tgaaggtcaa
    30961 ggtcgccgac gcgaaccgga acgcggccgc gctcgtcgac gagaaggtcg tcgtccccga
    31021 ggcgcccgac caggtcctct cgcccgcgga gaaggcggcg cgcgaggagg cgaaggcggc
    31081 cctcgcccgg ctcgtggccg agccgaacga ccggggcgtc ctctccttcc tccccctcgc
    31141 gcgggagttc gcgacgggcg tcgtccggtt cgagacgcgg gcctcgtccg acgacatcgc
    31201 gtacgccgag ttcttcctcg acggcaggaa ggtcgtcacc gaccgccgtc cgccgttcga
    31261 ggccgacttc gacctcggcg agctgccgcg caagaggacc gtgaaggtcg ccggcttcgc
    31321 gaaggacggc cggctccttt ccgaggacga gatgatcctg aacgaggggc gcgaggcgtt
    31381 ccgcgtccgg atcacctcgc cggagaaggg catccagctc gaggggccgg tccgcgtcgt
    31441 cgctgacctc gccgtccccc agacgaagaa gctccagcag gtggagttct gggtgaacga
    31501 gacccgtgcg gcgacgctct accagccgcc gttccagcag gtcgtcgacg tgccgcggca
    31561 gaaggagatc gggttcctcc gcgtcgtggc gacgctcgcg gacggcagcg tcagcgagga
    31621 cgtccgctac ttcaacgccc cgaagtacct ctcggaggtc gacgtgcagg ccgtcgagct
    31681 ctacacgtcg gtcttccgga agggccgccc ggtcacgggc ctctcccggg agagcttccg
    31741 ggtcctcgag gacggcgcgg agcagacgat cgacggcttc gaggtcgtca cgaaccttcc
    31801 cctcgcgctc gggatcggcg tcgacacctc cggctcgatg gaggagacgc tccccgaggc
    31861 gcagaaggcc gcgaacgact tcctccggaa cgtcatgacg ccgcgcgacc gctgcttcct
    31921 cgtgacgttc gacaacgagc cgcagctcgt ctccggcttc accaccgacc gggaccggct
    31981 cgcccaggcc ctcgccggcc tgcgcgccca gggctcgacc tccctctggg acgccgtcgt
    32041 ctacggcctc taccagtacc aggggacgaa ggggcgaaag gcctacgtcc tcctgaccga
    32101 cggcagcgac cgggcctcga agttcacgtt cgaggccgcg ctcgactacg cccgcaagtc
    32161 gggcgtggcg atctacttca tcggcctgaa gatcggcggc acgcagttcg acgtgcggac
    32221 gaaggtcaac aagatggcgc gggagacggg cggtacggtc tactacgtcg actccgcccg
    32281 ggcgctcgcg ggcgtctatg ccgagatcga cgaggagctc cggagccagt acctcctcac
    32341 ctacaccccg ccggcccgtc agggggcgaa gtggcggaaa gtggaggtgg aggtgacccc
    32401 ggacgacctc gaggcccgga cgatcagcgg ctactacccg taggcgcggg gcctccgtag
    32461 actccgccgg cgtgactcga ccgccgctcc tcctcgcctc cgcctccccg cggcggctcg
    32521 agctcctccg ggcgctcggg ctcgagccgc tcgtctgccc ggccgacgtc gacgagacgc
    32581 tccgccccgg cgaggaccct cacgaagcgg ccgaacggct cgcgcgagcg aaggcggccg
    32641 ccgtcgcaca gggagccccc accggcgccg tcgtcctcgc cgccgacacg atcgtcgtcc
    32701 tcgacggcga ggccctcggc aagccgcgcg accgggccga cgcgcgccgg atgctccacg
    32761 ccttgcgcgg ccgcgcgcac gacgtcgtca cgggcgtcgc cctcgcgcgc gacggccggc
    32821 tcgtctccgg ccgcgagacg accgaggtcc tcttcgcccc gatgaccgac gaggaggtcg
    32881 acgcctacgt cgcctccggc gagccgtccg acaaggcggg cgcctacgcc ctccaggggc
    32941 tcggcggcct cttcgtcgag cggatcaccg gcaccccttc gaacgtcatc ggcctcccct
    33001 tccggctcgt ccgccggctc gggtccgagg tcggcctgca gatctagcgg ggagccgccg
    33061 cggggaggct cctcgagcct ccccgatcca cttcagagct cgacctgcag gccgagctcc
    33121 acgacgcgcc cgggcgggat gttgaagaac gccgtcgccc gccccgctcg aggggaggcg
    33181 gcgcagccgc cgcggggagg ctcgtggcga gcctccccga tccacttcag agctcgacct
    33241 gcaggccgag ctccacgacg cgcccgggcg ggatgttgaa gaacgccgtc gcccgcccct
    33301 cggatcggga catccaggcg aagaagtgct cccgccagag cgccataccg gggcgcttgc
    33361 tcgcgatcag cgtctcgcgg ccgaggaaga acgtcgtctc catcggctcg aaccccttcc
    33421 cgtcgagctt cagcttccgg agcgcgcgcg ggacgtcggg gttctccatg aagccgtagt
    33481 ggagggtgac ccgccagcag ccggggccga ggtactcgag gcgtgagcgc tccttctcct
    33541 tcagccgcgg cacctcgtcg gtgacgaccg tcaggaagac gacccgctcg tggaggatct
    33601 tgttgtgctt caggttgtgg aggagcggcg gcgggatccc ctccgggatc cggtccatga
    33661 agacggccgt cccccgaacg cgcgtcggag gacgcttctc gacgtcctgg aggaagacgt
    33721 cgaccggcag cgacgcctcg cggaggcggg ccgagaggat ctgccgcccg cgcttccagg
    33781 tcgtcatgag catgaagacg aacgtggcga cggccagcgg gaaccagccg ccgtggaaga
    33841 ccttcacgag gttcgcgccg aggaacgaga ggtcgatgca gaggaagaga ccggcgacgg
    33901 gcagggcgat cgccttcggg acgttccagg cccgacgggc gtggacgtac atgagcgtcg
    33961 tcgtgatcgc catggtcgtc gtgaccgcga tgccgtaggc cgaggcgagc gcgctcgagc
    34021 tcccgaacga gaggacgagc cagacgcagg cgatcgcgag gatccagttg atcgagggaa
    34081 ggtagatctg cccgatctcc ttttccgacg tatgggcgat cttcagccgc ggcatgtagc
    34141 cgagctggat cgcctggcgc gtcagggaga aggccccgga gatgacggcc tgcgaggcga
    34201 tgacggaggc gatcgtcgcg agcgcgacga gcggcaggag gccccacttc gggaccatcc
    34261 cgaagagcgg gttcgccgcg gcgtcgggct tcgtcaggag gagcgccccc tggccgaagt
    34321 agttgaggag gagcgagggg agaacgaggc cataccaccc ggcccggatc ggcttcgcgc
    34381 cgaaatgccc catgtcggcg tagagcgcct cgccgccggt tatcacgagg aagaccgcgc
    34441 cgaggaggag gaagccgacg acgccgttct gctggaagaa tcggacggcg tggagcgggt
    34501 tcaccgccga gaggacctcc ggcgccgtcg tgacgtgcac cacgccgagg atcgcgagga
    34561 ccgcgaacca gacgagcatg atcgggccga agatcgcccc catccccgcc gtccccttct
    34621 tctgcaggga gaagaggacg aggaggacga cgacggcgat cggccggatg tacgggtcga
    34681 acgcgggggt gacgatgcga agcccctcga tcgaggagag gaccgtgatc gccggggtga
    34741 tgatgctgtc gccgtagagg agcgcggcgc cgaagagccc gacggccacg atgaggaacc
    34801 cgcccccggc tcccctctcc ccggagggac ggaggagcgc catcagcgcg aggatgcccc
    34861 cctccccccg gttgtcggcg cggaggacga tcgcgaggta ctggaccgag atcgtcagga
    34921 cgagcgccca gaaaatgagg gagaggatgc cgaggacgtt ccccggggtc ggcgggatcg
    34981 ggtggtgccc cgtgaagcac tcgcggaggg cgtagagggg gctcgtcccg atgtcgccga
    35041 agacgacgcc gagcgctccg agcgtcagga ccgcgaggta cttcccctcc ggcgccggcg
    35101 ggaggtgggt cttctccgtg ccggccgagg tggcttcgaa ggccccggag ggcgaccgcg
    35161 atggggggtg cgaggccgaa gactcgccgg agtccgactt caacggggcg gaagtctagc
    35221 ccaggcggcc atccggtgcc cggccgggtt cggcgggttc tcggcgggag gcgaggaggg
    35281 cgtcgatctc ggcgtccttc tcgaggaaga ggtcgcgaac ccaggcctgg acagagccgc
    35341 ggaacgcggc gtcatcggag tagtccccct cgaaccagcc gcccgggatc gccctctcct
    35401 gcaccctcac gacgacctcc gggagaccgc gcgagatcag gtcccagaag gtcggcgagc
    35461 cgtggggata gacgatcgtc acgtcgacga tgccggcgag ctgcggaccc atcgcggacg
    35521 ccgcgaacgc gagcccaccc gccttcggca cgaggaggtg ccggaacggc gacccgccgc
    35581 gcgcgtgctt ggccggggtg aagcgcgtcc cctcgacgaa gttcaggatc gcggccggcg
    35641 cgtgccggaa cctctcgcag gcgcgccgcg tcgcctcgag gtcttcgtgg cggaggtcgg
    35701 ggtttcgctc gagcgcctcc cgcgagtggc gcttcatgaa cgggaagtcg agcgcccacc
    35761 aggcggcccc gaggaacggc acgcggatca gctgctgttt caggaagaag cgcaggaacg
    35821 ggatccgacg gaggaagacg ccctggagga ccgggatgtc gacccacgac tggtggttcg
    35881 agacgacgag gtaggagacg ttccgccgga gcccctcgag ccccgtcacg tcgtagacgg
    35941 tgggctgggt gagcttcagg ctgaccgtgt tgacggcgat ccacgcctcg ccgatcgcga
    36001 ccaggagccg cgaggcggcc ctgcggaacg cggcgatcgg gacgacgagc ttcagcagcg
    36061 tgaccaggta gaggagggcg caccagaaga aggtgttcag cgcgaagagg aggaacgtcg
    36121 cgctcccggt cacgaggcgt cccgcggaca gggccgaggc tggggagaac ggcatcgcgc
    36181 gaaatggtac ggccgatgcc ccgtccgagg ctcagagcgc ccggacgacg acgacggcga
    36241 cgtcgtccgc cgggcgcgag cccccggtga cgcgcgcgac ctcgcggacg atcgcgtcgc
    36301 ggatctcgcg cgccgtccgc gagccggtcg gcaggccgcg caggaaaccg gcgagcgcgt
    36361 cgtagcccca cggcgtcccc gcgcgacccg ccgcctccgg gacgccgtcg gtgtacagga
    36421 cgacgacgtc gcccggcgag agggagaccg tccgggagcg gtagcgtcca cgcgcgccgg
    36481 cgccgagcgg gaacgtgctc ccctcggagg cgaggtaggt cgccgtgtcg cccgagcgga
    36541 ggagcggctc gcagagcccg gcgttcacga aggcgagctc gcgacgctcg gggtcgagcg
    36601 tcgcgaggca ggccgcggcg aacatcctcc tcccgatctt ggcgcggagg gcgccgttga
    36661 cctgctcggc cacggcctcg agcggttcgc cccgcctcac gagcgcgccg accatcccgc
    36721 ccgacatcgc cgccgccatg gcggccgggg tcccctttcc cgagacgtcg ccgacgacga
    36781 tcccgagctt cccgcccgct tcgccgctcc tcacgtagtc gaagaagtcg ccgcccacgt
    36841 cgagggctgg gatgcagacc cccgagacct cgaacccctc gacctcgggg tcctcgtgcg
    36901 gcatgacggc catctgcgcg tcgtgggccg cccgcatcgc cgccgcgagc cgcaccgtcc
    36961 gcatcctccg ccggagggcc acgacgacgg cggcggcgag gccgagggcc gcggcgaggc
    37021 ggaaccacca ggtgccccag aacggcggcg tcacgacgat cttcaggtgc gcctcgcgct
    37081 ccccccagac gccgtcgggg ttcgcggcct tcacgcggaa gaggtaccgc cccggcgcga
    37141 gccccgtgta cgtcgcgacg cgccggtccg cgcccgtcgg gatccagtcg cggtcgaagc
    37201 cgtcgaggcg gaacgcatag cggagcttct gcggggccgc gaagtggagc gccgcgaact
    37261 cgagcgagac gacgtcgtca tcgtgcgaga ggacgacctc gtccgcgtac gtgacgaagg
    37321 cgggcaggag cgtcctcccg tccacggggc ggccggccgg gacgggccgg ttcgcgagga
    37381 ggagctccgt caggacgacg ggggccgcct ccgtccggac ggcgatctcc gaagggaaga
    37441 agacgtcgaa gccccggatc ccgccgaaga acatctcgcc gcgcgggctg cggtagaagg
    37501 cgccaccgtt gaactcgtcg ctctgcaggc cgtccgtctc gtcccaggcg cggaacgtcc
    37561 catcggccgg gtcgagctcc gtcaggccgc ggttcgtcga gatccagagg cgcccccgct
    37621 cgtcctcgag gatcccgtag acggaatcgc tcgagagacc atcgcgcgcc gtgaagcgcg
    37681 tgaagcggcc catcgcgcgg tcgaggcggt tcaacccgcc cccgtacgtc ccgacccaga
    37741 agctcccggc gcggtcctcg tggagggcga agacgaagtc gttcgaaagg ctggccggat
    37801 cagcggggtt cgcccggaag cgcgtgaagc ggcccgtcgc acggtcgagg cggttcagcc
    37861 cgcccccctg cgtcccgacc cagagcgcgc cggccgaatc ctcgaggacg caccggacga
    37921 agtcgttcga gagcgtcgcc ggatcggcgg ggtcgttgcg atggtggacg aagcgccccg
    37981 tctcgcgctc gagccggtcg agccccccgc cgtaggtccc gacccagagc gtccccgacc
    38041 ggtcctcgta gagcgtccgg agctcgtcgt gggcgaggga cgccggatcg cgcgggtcgt
    38101 gccggtggtg cgcgaagcgg ccggtcgccc ggtcgaaccg gtcgaggcct ccgccgttcg
    38161 tcgcgaccca gaggttcccg gccgcgtcgc cgaggagcgc ccggacggtg tcgtgcgcga
    38221 gcgaggccgg gtcgcgcgga tcgtgccgga agccgcggaa gacgtcgcgc gcgcggtccc
    38281 atcgctggag cccaccggag tcggtcccga cccagacggt cccgtcggcg tcctcgtgga
    38341 acgcccagac gatgtcgtgc ccgagcgagg tcgggacgtg cgggtcgcgg cggacgttcc
    38401 ggaacttctt ccggccgagg tcgagcgtgt tcaggccccc gccgtaggtc ccgatccaga
    38461 ggaccctcga ccggtcctcg aggagggaga agacgcggtc cgtcgcgagg ctccgcggga
    38521 cggacgggtc gtgcgagagg acctccactg tctccgtctt ccggtcgtag cgggcgaggc
    38581 cggcgccgtc ggtcgcgatc cagaggaacg cctcgtggtc ctccgcgagc gccttcaccc
    38641 tctcgcccgg aaggcgcgcg aagcgcggcc gcggaacgag ccgccccgac tcctcgtcga
    38701 ggaggaagag cccgccgccc cacgtcccga cccagaggtg accggagcgg tcctccagga
    38761 gggcgtcgac gagctccgcg cctctcccgc cgtccgacgg gacgtggacg aaccggcgcg
    38821 acgccgcgtc gaaccggtcg agaccgccgc cgtacgttcc gacccagagc gttccgcgtc
    38881 cgtcgacgag gagcgccctc acgtcgtcgt gcgcgaggct cgccccgtcg cgcgcgtcgt
    38941 gccggaagcg ctcgaaccgc cccgtccgcg ggtcaaggcg cgcgagcccg ccgccttgcg
    39001 tcccggccca gatccgcccg tcgcggtcga cgcggacggc gcgcacgagg tcggcgggga
    39061 ggctcgtcgg gtcggccggg tcgttgcgat acgccgtgac gcgcccggtc gccgggtcga
    39121 accggttcag cccgccgccg aacgagccga tccagagcgc cccctgcccg tcctcgtcga
    39181 ggcacttcag gtcgttgtac gggaagcttc gcgcctcgcc cgggacgttc cggaagaccg
    39241 tgaagcgagc tccgtcccag cggttcaggc cgtcctccgt cgcgagccag aggaagccga
    39301 gacggtcctg gtggatcgct tcgacgatgc tctgggatag cccctcgtcg agagagaggc
    39361 gccccagctc ccgcggcgcg gcggggagcg ccgcgaggac ggcgccgagc gcgaggaggg
    39421 cgaggcggcg gagcctcatg ctctccgtta cggcccggcg cgagacgggt tcgtcctgcg
    39481 atgaggtcgc gccttgacga gcgcgccccg gaccggcaca ttcgcggcct cattcgaaag
    39541 cgcccgccca cccgccgcac ggaggccgtc gacgtcctcc tcgtctcgat gccgttcggc
    39601 cccgagatgg cgccgtccct cggactctcc ctgctgaagg cgggcctcgc gccgctcggc
    39661 gtctcgtgtc gcgtcctcta tctcacgctg agcttcgccg agcggctcgg cgtcggcccc
    39721 tacacgcgga tcgcgaccga cggcacccgt tcgatccgcg agctcgcggg cgagcacgtc
    39781 ttccgcgagg cgctcttcgg ccgcgacgtg gaggccgacg cccgctggga gcgcgacgtc
    39841 ctcctcgccc gggcctgctg gccctcgaag gagctcgcgc gtccggtccc gccgtcgttc
    39901 gtcggggccg tccggcgcgc acgaagcctc gccggcccgt tcctcgagcg ggccctcgac
    39961 gaggtcctcg cccaccggcc gcgcctcgtc ggcttcacga gcgtcttcca gcagcacgtc
    40021 gcctcgctcg ccctcgcgcg ccggatcagg accgccgcgc cggagaccgt cgtcgtgatc
    40081 ggcggcgcga actgcgaagg ggtgatgggg gccgagacgg cgcggagctt tccgttcctc
    40141 gacgccgtcg tctcgggcga gggagacctc gtcttccccg agctcgtcgc gcgcgccctc
    40201 gccggccgcg cgctcgacga cctccccggc gtcgcgactc cggggaacgt cgaagcgcac
    40261 ttcgccgcgg gccgcttccc gaacgccccg tcggtcacgc ggatggacga ccttcccgtc
    40321 ccagacgacg gcgactactt cgagcagttc gcgcagagcc gtttcgccgg gacctggacg
    40381 cccggcctct tcctcgagac ggcccgcggc tgctcgtggg ggcagaagag ccactgcacg
    40441 ttctgcggcc tcaacgggac gacgatggcc ttccggagca agtcgcccgc gcgcgccctc
    40501 gccgagctgc gcgacgtcac ctcgcgccac cccgagagcg acctgcaggt cgtcgacaac
    40561 atcctcgaca cgcgctactt cgagtcgctt ctccccgagc tcgcgaagtc gccgccgccc
    40621 tcgcccgtct tctgggagac gaaggcgagc ctgaagaagg agcaggtccg cgcgctcgcc
    40681 gaggccggcg tgacccgcat ccagcccggc gtggagagcc tctccgacgc cgtgctgaag
    40741 ctcatgaaga agggcgtcac cgcgctccag aacgtccagc tgctgaagtg gtgccgcgag
    40801 ctcggcgtca cgccgtactg gaacctcctc tggggcttcc ccgacgagga gccatcggag
    40861 tacgcgcgga tggcgcggct cgtgcctctc ctcgcgcacc tcgcgccgcc ggcgggcgtc
    40921 gccggcctgc ggctcgaccg cttcagcccc gggttcgacg acgcagatcg gctcggcttc
    40981 ctcgacgtcg cgccgatgcc gtcgtacgcg gcggtctacc ccttccctcc ggaccgactc
    41041 gcgaacctcg cctactcctt cacgttcgcg cgccgcggcc cgggagaggc ggccgagtac
    41101 gtcgcgcccc tcctccgcgc cctcgcgcgg tggaagcgcg tcgccggcgc gagcgccctc
    41161 ttctccgttc cggcgggcga cgcgctcttc gtcttcgacc tccggaccgc cgcgcggcgc
    41221 ccgctcacgg tcctccgcgg agaggcgcgc gccgtccacg acgcgtgcga cgcctcgacg
    41281 aacgtccgcg cggtcgtcga gagagtccgc tcggaatgcg gctttgcgcg cgaggcgacg
    41341 gagcgtgcgc tctccgcgct cgtgcgcgac cgcctcctcc tcgaggacgg agaacgtcgt
    41401 ctcgctctcg ccgtcccgct cggacgcttc gctccggacg gacgagcgag cgcgcgcttc
    41461 catcgcctcg cgcgaagaat cgggcgaagg acacgtgacg gaatcgcttt tcccgttcac
    41521 aatgcagcgg tgacgtttcg gacacggaac ggggacgacg cgccgcacgc cccgcgagac
    41581 acgcggacga cgtcttcctc cagcgaagtt ctactcgacc gcttctccgt gaatcgtcgc
    41641 ggagagctgg tcttttcctg aacccccgtc ctcgagggcg gaaccgaaag gagatccgat
    41701 ggcaacgaag aaggctgcga agaaggtcgc aaagaaggcc gtgaagaagg tcgccaagaa
    41761 ggcgccggcc aagaaggccc cggcgaagaa ggccgtcaag aaggtcgcga agaaggccgt
    41821 caagaaggcc cccgccaaga aggtcgccaa gaagaaggac gtcggcctca tctgatcgac
    41881 cgtcgcgccg gagccggtcc cctcgcgggg ccggctccgc gccgcgatct cccggcgccg
    41941 cctcggcccc ctacttcagg ccggtgacga ggtggaggtc gttcctgtac tggaagaagc
    42001 tgtagaccca ggagcgtccg tcgggcgtca cctgggccgc gacgatcgac tcgacgcccg
    42061 ccgggtccgg gggcgcgatc gtcgcgacga cctcccgcgt ccgcgtggcg agctcgaccc
    42121 gccggatgcg ggcaggaagc tcgtcgaagc ggaagacgaa cagtgagccg ccgtcgacgc
    42181 tccagcgcat cggcacgtct ccgggctcga ggcccgggac gagcctcggc tcggctccgt
    42241 cgatcgggag gaagaccacg cgcccttcgc cgtcggacac ggccgcggtc cgcccgtccg
    42301 gcgagacgac gtcggacggc ggagcgaacc gcagcccctc gcccggcagc gcgcgcggcc
    42361 cgccggcctc cgtgctctgc acgtagagcc ggaccggctt cccctcctcg ttcgccgcga
    42421 gcagcagcct ctctccaccg gggaagaacg ccgccgcctg gtagctcgtg atcggcccgc
    42481 gatcgagcgt cttcgcacgg cccggacccg tcgggaggag gacgacgctc ggcgggctcg
    42541 acgggacgat cgccaccgcc cacttcccgt cgggcgagag cgggccggcg gtcccttcgc
    42601 cgaggcgcgc cgcggcggac gcctccgtcc cgcggtagta gaccgagtac gtcccagcgc
    42661 ctcctccggc cccctgctcg gcgaacagga tggcgcgacc gtcacgcgag aggtccgaga
    42721 cgaccgtcgc gtcgagccac gagaggtccc ggtccgcgg
//
LOCUS       DAOONL010000023        42543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_668_length_42543_cov_15.658280, whole genome shotgun sequence.
ACCESSION   DAOONL010000023 DAOONL010000000
VERSION     DAOONL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 42543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_668_length_42543_cov_15.658280"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLP50_05670"
     CDS             <1..695
                     /locus_tag="PLP50_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPA51074.1"
                     /translation="GLEGFKKEFARDRAELASWKVADPGRISLEETIENAKPSILIGV
                     SATPGTFTEAIVRKMAALNQRPLVFPISNPTSKAECTAEQAVTWSDGRAIVATGSPFA
                     PVVHNGVTHRIGQCNNAFIFPGIGLGVCVSKARRVSDGMFLAAAQALATHVTEADIAE
                     SAVYPQVATIRDCSHSVACAVIRRAVAEGLAEPEILNGLEETVRRAMWFPEYLPIRYE
                     PTGLPTRRHAHA"
     gene            complement(783..1508)
                     /locus_tag="PLP50_05675"
     CDS             complement(783..1508)
                     /locus_tag="PLP50_05675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPA51075.1"
                     /translation="MPEETPRPETTDASRRDFLKGAAATAVAAGALGFPAILKGAEAL
                     ALAPLPWPDTALEPVISAKTLSFHWGKHHRGYVDALNGLVKGGPLEGKPLAEIVRASA
                     QDASARAVFNNSAQAWNHDFFWRSLRPKGGAAPSGALAERIEASFGSLDTLKKELVAA
                     GLGQFGSGWAWLVADGAALKVVKTANADTPLTTGQTPLLVLDVWEHAYYLDYQNRRKD
                     YLTAVVDGLLNWEFAASNLPKKA"
     gene            complement(1565..2536)
                     /locus_tag="PLP50_05680"
     CDS             complement(1565..2536)
                     /locus_tag="PLP50_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HPA51076.1"
                     /translation="MTHAVRIHRPGGPEALSWDEVRVGPPGPGEARVRHTAIGLNFID
                     TYHRSGLYPLPLPAVLGSEGAGVVTEVGPDVTEVKAGDRVAYAGPVGAYSEERLVAAH
                     RLVPLPDDVDDRVAAAAMLKGMTARYLLRKTHSVEAGETILLHSAAGGVGLIAAQWAR
                     SLGATVIGTVSTDEKAELARAAGCHHVVVTSREDLVQRVDEITGGVGVRVVYDSVGRE
                     TFEASLDCLAPLGLMVSFGNSSGPVPPFAPLLLSQKGSLFLTRPTLMAYTARREDLLE
                     TAADLFSVIRSGAVSVRIGATFPLREAAAAHRELEARRTTGSTVLVP"
     gene            2609..5635
                     /locus_tag="PLP50_05685"
     CDS             2609..5635
                     /locus_tag="PLP50_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA51077.1"
                     /translation="MALPIARRAWLAAALALLLADTRALSLSPERQLNQYAVSTWQTA
                     DGLPMNSVLAMTQAGDGSLWIGTEEGLVRFDGAEFEVRDRGNTPSMDAHNVQALCTDE
                     AGAVWAGTYGAGLLRWDGRAFSRLAAESEPERSRPIRALARGDDGSIWIGTAGGLERL
                     VDDRRTRFAADPGLASSAVLAISPGRKGRVWVATVAWLGFVENGALHRLVPEPAPGGV
                     VLSLAEGPDGALYVGTDRGLFRISTDHRACERIRGLSNDFVSALAVDQNGALWVGTNG
                     GLHRIGRDGRIERLTTGDGLSSDMIQTLLLDREGNLWFGTRGGGLGRLRDGDFVTFGG
                     AREGFVVDTVLSARSGGVFVGGWNGFLARLGPDGRFEPLASPAALAGSSIRALFEEAD
                     GTLLVGAWNGLYRARDGHVERVPDRGLIRNVRTVHVDQEGAIWVGLEAEGLVRLQAGK
                     VRRFTTADGLGGNEVRALLEDRDGTLWVATYGGLSRRTGESFTTLTTRDGLRSDLVRS
                     LALDADGAIWIGTYGGGIARLDRGVITSWDTRDGLYTDVAYAITEDDAGDFWISSNRG
                     VYRVARADLEARASGGGPRLRCRSFGIADGMASEECNGGDPAVARTADGRIYFPTVRG
                     VVAVAPTRLLPTAPPRVRIDSVVAGAIAVDHRETAEVPAGTRRLELRWAALGFLPPLK
                     TEFRYRLDGFDDEWVEAGTRRVATYTNLPPGSYSFRVVAGLDGSFGSAETRLPVRVGR
                     LWWQTWWARAALGFALAGLLGGAYRLRTGSLARDRRRLEEAVAARSAELAEANRALEE
                     AVVTDPLTKARNRRFLDMALDSERELAVRSSESKDSCRNRDLILYLVDLDRFKSVNDR
                     HGHRTGDLVLVETARRLQGALRRTDFLVRWGGEEFLVVARGSERRQADVQARRLLEAV
                     GGSPYSLGDLSLVQTCSIGWAPFPWLEERPRDATIEEVLGFADRALYEAKRSGRNGAV
                     GYVAGGDVGQKGSGESFEEQPGRVVEVVRTEGPRTG"
     gene            complement(5661..6290)
                     /locus_tag="PLP50_05690"
     CDS             complement(5661..6290)
                     /locus_tag="PLP50_05690"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HPA51078.1"
                     /translation="MKWTTLPAFAAATLAAALLFPATPAAAHCDSVEGPVVVDAKAAL
                     EKGDVAPVLKWISAAQEPAIREAFRKTLGVRKLGAEAKEVADTAFFETLVRLHRETEG
                     AAYTGLKRGVKEPAFIGQLEAALTSGSVDAFSKLVGEHAAAGIQGRFSKALAAKKSAD
                     ASPADGRAYVAAYVELMHYTKAIVEAVHAQHAGPAAHAAPAKGAGHTCD"
     gene            complement(6404..8707)
                     /locus_tag="PLP50_05695"
     CDS             complement(6404..8707)
                     /locus_tag="PLP50_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPA51079.1"
                     /translation="MSTNLPGNDNRRWALLGGVLLFTFLVLGFFGREVYRQAPPIPER
                     VVTPDGATLMTKDDILTGQQVWQSTGGQQLGSIWGHGAYQAPDWTADHLHREATALLD
                     VKAEAEGAQDFASLSFDRQAALKAALVREMRANTHDAATGVVTISAERAEAYRRAAAH
                     FDALFGGSPDLKPLREDYAMSETTVPDAARRKALTAFFFWTSWAATTERPGQAITYTN
                     NWPHEPLVENVPSGANVVWSLVSIALLLAGIGAVVWWTAFREKPEPAVTAPPVDPLVG
                     LTLTPSMRAVGKYVVAVLGLFAVQVGLGALLAHYTVEGQALYGLPVGEWVPYALARTW
                     HVQTAVFWIATAFLAAGLFLAPVIGGHEPKYQRLGVNVLFGALLLVVVGSLAGEYLAI
                     HQVLPLDLSFWLGHQGYEYVDLGRVWQIALFVGLVLWLVLMLRAILPAARRRDDARSL
                     VLMFTGATTAIGLLYGAGFFFTAKTHLSVMEYWRWWVVHLWVEGFFEVFATAAIALLF
                     AKLGLVDKKHAGAAVIASSALFLLGGIPGTFHHLYFSGTPVSILAVGASFSALEVVPL
                     TLIGLEAFRTYKLQHAAPWMAKYRWPIVFFVGVAFWNLVGAGVFGFLINPPLALYYMQ
                     GLNTTPVHAHTALFGVYGLLSLGLVLIIARKLSGVRAWKETQLKWAFWGMNVGLGLMV
                     VLSLLPIGLAQTWASVEHGLWYARSADFLQLPALQALRWLRILGDTVFLGGVAFLLWF
                     FVGLLTGWSYEKAEEPGFDDDTAPESA"
     gene            complement(8722..9399)
                     /gene="ric"
                     /locus_tag="PLP50_05700"
     CDS             complement(8722..9399)
                     /gene="ric"
                     /locus_tag="PLP50_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPA51080.1"
                     /translation="MNLPLQPESTLADLAVAYPAASRVFRRHGLDYCCHGRRPVSEAC
                     AERGLDAAALLSEVAAEARTSGDVETMADQPIPAIVERIIGWYHARLRTELPLLVQMA
                     RKVEGVHAEKESVPRGLAAHVESLSQELIDHLDKEEQGLFPMLLSGRGGQAAAQVQSL
                     EREHEDVALALQKTRALTTDLVPPIEACTTWRALYLRLDELEAELMEHVHLENNILFR
                     RALCADM"
     gene            9519..11075
                     /gene="norR"
                     /locus_tag="PLP50_05705"
     CDS             9519..11075
                     /gene="norR"
                     /locus_tag="PLP50_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HPA51081.1"
                     /translation="MSSNSQQEPLEVLLEIVGDLTASLAARDRYERLLSAVRRLVPYD
                     AACLLRLQGDDLVPLAGHGLVPGALERPYPRSRHPRLEAILRSEAPLLFPHDSPLPDP
                     FDGLLVADPHALDRIHACLGCALTADGEVVGALTADALDPEAFDGLDGRLLATLGALA
                     GAALRTASLIEALERKAERGTRVARELQRSADETSGAAILGASAAVAALLSEIGVVAA
                     SDLPVLVTGETGVGKELVARHVHSGSRRKDEALIHVNCAALPESIAESELFGHVQGAF
                     TGAVRDRAGKFEVADGGSLFLDEVGELPLSLQPKLLRALQQGEIQRIGSDRPHRVDVR
                     VIAATNRDLAAEVARGRFRADLYHRLAVYPIRVPALRERREDVPILATHFADAARRRL
                     GLGPLRLSEEARAALAAAEWPGNVRELENVVSRAVLRAAAGKPPGAPVLVQAAHVDAS
                     STATAPERAPAASPDAARPLREQVEDFQRRRIREAVERHAGNQAAAARELGMHRANLH
                     HLARRLGLRG"
     gene            11126..11734
                     /locus_tag="PLP50_05710"
     CDS             11126..11734
                     /locus_tag="PLP50_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPA51082.1"
                     /translation="MDTSFVSSTILLVLITDPLGNIPFFISALRHVRPERRRVVVLRE
                     CLVAFAALLLFLLAGRPILRLLHLSEDALRISGGVVLLLIAIRMIFPERGARLGEEEG
                     EDREPFIVPVAIPLIAGPSAMATVLLMSTPDPLRMLSLAGSLTVTIAITAVVFVSSTR
                     IQKVLGDQAIMAMERLMGLVLTAIAVEMLLGGVASYIRRGPG"
     gene            complement(11710..13281)
                     /locus_tag="PLP50_05715"
     CDS             complement(11710..13281)
                     /locus_tag="PLP50_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPA51083.1"
                     /translation="MSVRRRLASVPFLVLLALLAPSASAGASSDSKPEDDGIRLGVAR
                     LAWLEGDVAVARGDAAEGWRPGAVNDPLTTGDRLRVAAGGRAEVQLRGAVIHLAPGTE
                     LAFPDLSLEDRSVTLSAGTVSVRVFDVRGAGSLEVATPSVSVALELPGDYRFDVGDSG
                     GTAISIRRGRARAATAEGGVTLGWGERMRVTASPRPEYDVVTLGRGDAWDRRVDQRSA
                     RFRLVRSAAHVHADVLGIDDLDAWGDWEETAERGWAWFPKVSLADWMPYRSGRWIWRE
                     PWGWAWLSDEPWGWAPYHHGRWAIVRSRWAWLPDEPKDPGPSFLPAVVTFVGAGPGGV
                     GPLGPDGYVGWFPLAPGEPLQPWWVRVEGPTGDSSFRYLHRDRALYLPHAIFARGRFG
                     ESDLVHDAALLRDLRNAPVVRSGLPVDPERERVRTPPEASGAPASAGLRSALPIRRTK
                     TPAPAGRATPSPAPTPARAAPVPPSRSPRLTPRTPEASPAAAPPWASVPTPAPSAGAA
                     RSAPRPTPQPGPRRM"
     gene            complement(13390..16362)
                     /gene="gltA"
                     /locus_tag="PLP50_05720"
     CDS             complement(13390..16362)
                     /gene="gltA"
                     /locus_tag="PLP50_05720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPA51084.1"
                     /translation="MYQIVKREQFSDVTFLWDVHAPDVAKAARPGHFVMLRLDEGGER
                     IPLTVADYDPEWGTVTIVIQALGKTTREMMRAYKEGDTFADFVGPLGLESHVGKVGHV
                     VLVGGGLGVAPVFPQLRAFKEAGNRTTGIIGFRNKDLVFWEDRFRRYCDDLIVCTDDG
                     SYGKPGFVTAALQEVLEKEKPDLVVAIGPLPMMRACSEVSRPFGVKTMVSLNAIMVDG
                     TGMCGSCRVTVGGETKFACVDGPDFDGHLVDFPELQLRQERFKPQESRAKDDFSKVCS
                     IEEQLFEMNKRNYKKFKDLDEHAVKMPERNALERSRNFEEVNLGYTMADALQEADRCI
                     QCARPTCVAGCPVSIDIPRFIRHLLVRDLEGALSVIQESNLFPSICGRVCPQESQCEI
                     QCILEKKMEPVAIGRLERFVGDFAPLPDVKPPENAGTLGKVAVVGSGPAGLACAGDLA
                     RKGASVTVFEALHVVGGVLKYGIPSFRLPRTTIDREVKALEKLGVKFETNKVIGKTFT
                     IDQLKGEMGYDAVFIGTGAGFPMFLGIPGEHAGQVYSANEFLTRINLMHGNKFPFEDT
                     PIAMGKKVAVFGAGNTAMDCLRVARRLGAEEVHCIYRRTEAEAPARVEEIRHAKEEGI
                     HFNWLMSPTEIQVDEKGDVRAVVVEKMQLGEPDKSGRRRPVPTGEHETFACDTVIYAL
                     GTRANPVIARSAPDLGVRGEGYVNADAKTQATNLPGVFAGGDIVTGGATVILALGAGR
                     RAARAIETYLKTKEWPVVLEDEAAGAGVPVPAPVAAACAKCRRPFEPGEEDHLCCGGE
                     KVTWTCTSCQKVSEGFAFPYGLCPACGGTLSLGHDTDVTSEAAVAAIRHAFEIELGGV
                     TFYSRGAEELEAKDPDLAKLFRDLAEMERGHMDVLARRYHVEAPTESLDMHLEPAQIA
                     VYAGADIADLTGAALLRLAVHLEKRARAFFLDAGRKFPTGSHEWRLYRELEAEEREHV
                     DMLSTVLARIVAEKPVAV"
     gene            complement(16587..18335)
                     /locus_tag="PLP50_05725"
     CDS             complement(16587..18335)
                     /locus_tag="PLP50_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51085.1"
                     /translation="MRLNRPSHSTVHWFVLLVVSISALALSGPAASSTPEDVDPPLAW
                     RPFELGSYSRPVSTRVAEAQKAFDQGLVWAFAFNHDEAQRAFSEAARLDPALAMAWWG
                     VALVNGPHINNPAMSEVQSRTAWEALTKARALAGRATDVEKALIEALGARYSWPPPAD
                     RAPLDAAYAKAMLAVRDRFPADADVATLAAEALMDTRPWDQWTKDGKPQPGTAEVLAA
                     LEEARKLAPDHPGALHLTIHALEASPRPERAAEAADRLRALVPDSSHLVHMPSHIDVR
                     VGRWKEAAEANQRAMAADRRYQGRSGEIGFYRIYMAHNAHFFAYTAMMEGRREEALAT
                     ATGVVAGLDPAFVKENAAFADAFLTVVGEAQKRFGLWKEILAAKAPPEWLPVSTAHAH
                     FLRGVAHAALGEVAEAKRERAAFAAALPMVPKEFYWGSNPAADVLAVGLPYLDGEIAY
                     RLGDHAGAVEKLRSAVRLEDALKYDEPPAWTVPSRHALGAILLDAKRPAEAEAVYRED
                     LRAYPGNGWALRGLATALEMQKKSAEAEAVEARFAVAWARADTSLETSCFCVRKSRSD
                     APAGASALAQGRTLRR"
     gene            complement(18497..19477)
                     /locus_tag="PLP50_05730"
     CDS             complement(18497..19477)
                     /locus_tag="PLP50_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPA51086.1"
                     /translation="MKTMTPYDAIGEALAEEMRRDATVLAFGEGIATKRHDLVAEFGA
                     ARIRNTPLAEGIIAGTAAGAAATGLRPVVDLLFAPFLTFAMDELVNSAGKLRFLSGGQ
                     FSFPMVALAMTGAGWGVGAQHNHNVEAWFAHSPGLKVVMPSTAADFKGLLKSSIRDDN
                     PVAFFLDVALLHAPGEVPEGEHLVPLGKAAIRRPGSDVTLVSYAKTVLGCLAAAETLA
                     ADGISAEVIDLRTIKPWDEEAVLASVRKTGRLVVVHEASRTAGFGAEIAATVAEKAFD
                     ALKAPVVRLAGPDAPAASSWVLEQAFVPQADAIAAAARSAMARSMVLATA"
     gene            complement(19490..20452)
                     /locus_tag="PLP50_05735"
     CDS             complement(19490..20452)
                     /locus_tag="PLP50_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPA51087.1"
                     /translation="MSKDPTRPSPAELLEKMQLIRSFEERSAASFAEGRSPGTCTCVG
                     QEASAVGVVAALREDDLILTNHRSAGHLLARGADPGRLMAEVMGRATGYCGGKSGTLH
                     VSAKELGVILTSTIVGGELSLAPGVALSVSMKGGDEIVTCFFGDGAACEGVFHEALNL
                     AAVWKLPVLFVCENNEWQAFVHRRETMLTDDIASRASAYGIEGRTVDGNDVEAVLAAA
                     TEAAARVRATRMPFLLETKTYRLRGHFEPDDQGYVDKAELASWKARDPIAALRAKLLE
                     SGALTADAATAIERRVAERLDAAFSFAADSPWPSLDELTTHVYA"
     gene            20639..22108
                     /locus_tag="PLP50_05740"
     CDS             20639..22108
                     /locus_tag="PLP50_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA51088.1"
                     /translation="MRRAAALLALALGAAPAACREGVKPVRIAVHSAPQSFDPHLQNE
                     ILTSAVLANLYDGLTEFDEDSRVVPALASEWRNPDEKTWVFRLRPGVVFHDGRPLTAA
                     DVLFSLDRARHHSGSGLASYLVEIESVRMADPETIEIRTRRPFAALLAKLTSIAIVPA
                     DAPPEILDPIGTGSYRLARATADAVELVPATNGWRRGPLLPVTFVVDGDPRGRRERLM
                     TGDVDVATDLAEVAVKRLEASECCRVVAQPGSTVEYLRLSPAPPPFRDRRVRQAIDLA
                     IDRALYVAEAHGGIGQPAGQLCVPGVFGFAPDLKATARDLPRARRLLAEAGFGEGLDL
                     VLEHRPGRRGDILAGQLAEAGIRVTPLVTPWTELHPRIRSGAVDFYFGGVVAQTAEAS
                     DVLDSFVHTRDDSRGYGSTNHSRYSNPTADALIESASAALDLRRRRDLLQRAMRVVMD
                     DLYLLPIAGLYDIYGIRKEIVFEPRFDRRLPGRGIGRTR"
     gene            22105..23382
                     /locus_tag="PLP50_05745"
     CDS             22105..23382
                     /locus_tag="PLP50_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA51089.1"
                     /translation="MSRAIAAALAVCLAAVAACEGPGRPIRVAIHSAPVELDPHLQNE
                     SLTSGVLANVYEGLTELDEENRVRPKLASGWTNPDERTWVFSLRAGARFHDGRALTAG
                     DVVFSLERARRHPGSALASYLVEVESVRVVDDGRVEIRTRRPFASLLGKLTPVYVVPA
                     GSPERITEPDGTGPYRLVHADRRRLELVPVATSAERPEAPGPLHFVVEPDPARRLSAL
                     LDGEVEVAADLGEDAVDLLATTACCRAELLPGSTIEYLHLSDLEPPFRDRRVREAVHL
                     ALDRTLYLAEARHGLGQPAGQLAVPGLFGYEPGLRAPERDLARARALLEAAGYVEGFD
                     VDLEHRPGRRADVLVRQLGEAGIRATARESAWAELYGRLRNGEVGFYFGGVVSPRRAR
                     RATSWTATSTRARRTADTAPRTTRGTRTGRPTG"
     gene            23442..23576
                     /locus_tag="PLP50_05750"
     CDS             23442..23576
                     /locus_tag="PLP50_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51090.1"
                     /translation="MKVVMADLHFVPVAGLYQVYGTRRDVEFRPRIDLKLLGDRIRRR
                     "
     gene            23628..25490
                     /locus_tag="PLP50_05755"
     CDS             23628..25490
                     /locus_tag="PLP50_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA51091.1"
                     /translation="MAAPSTASSPALRSSSVTASPVAEVEARHVAHVLDERLGERGDG
                     TALRHLVGGQWQDISWRELAAKVRDLSSALVEAGVAEGDRVAILAANRPEWTMADLAI
                     LRVRGVSVPIHATSSSSQVAFILKDSGARVVFVDAGEPASKVVAALGAGVPLDRVIVF
                     GGGALPEGAAFADFEGTLARGRAAAREDEVAARLSRATPDDLLTLLYTSGTTGEPKGV
                     MLPHSAFTVTCTYHDLRLPPIGPDDVSLCFLPLSHIFERAWTFYILYRGAVTAYCEDP
                     KKVSEVLPQVRPTLMCAVPRLYEKAYTKIQERVRSSSAPKRALFRWAIGAGHAVSLRR
                     RAGEAVPQLLALRHALADRLVLAKIRAAFGGRPRLFPCAGAPLAPEIEEFFHSVGVFV
                     CQGYGLTETTATVTCHDPSRFHVGTVGSPLPGVEVRISEEGEVLVKGRTVMKGYWNRP
                     EETAAVLRDGWLRTGDAGEICEDGTLKITDRIKDLIKTAGGRYVAPQHVETTIGADPL
                     VEQVAVIGEGRSYVTALVVPAFEALEKWARAAGISFSGRDDLVRNERVLDLLKRTIEE
                     RTKDLAPWEKVRKFSLLTKEFSVEGGSMTPTQKVRRRAAAELYRDRIEEMYTGD"
     gene            25531..27138
                     /locus_tag="PLP50_05760"
     CDS             25531..27138
                     /locus_tag="PLP50_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPA51092.1"
                     /translation="MRRIAFALAVGLSFPTLIVRPVRAAEPAPVSPAELDAHIRFLAD
                     DLLEGRAPGTRGDGLAQAYIESVFRANGLAPAFGASYRQPVPIRIVVPDSKTTLTWTG
                     AAATAPAGRFGDDFVLAFPRPESAGSVSADVVFGGYGIEAKEWGWNDFEGSDVRGKLL
                     LLLSGEPGGDDPALFAGPILTHHGRWRTKLEVAAKRGAAGVVFVHTREGAGYGWEVVR
                     NGWTRTVAFAPDATTLSLEGWLTEAEATRAVRAGGVTLESLRAAAGKRGFRPIPLALK
                     LEAHGAPRYESVVSANVAGIVPGRGPAGTAPVVVVSAHHDHLGVGTPEEGDGIYNGAM
                     DNGSALAVLLALSRRIAARAGELPVDVLFLAPAAEEAGLLGSDLFARTPPVPLARIAA
                     NLNLEMSAVWGPARDVVAIGGSESALGAVVEAVAKKEGLRVAPEPAPDQGFFYRSDQF
                     SFAKAGVPGIWIDLGDDLVGTPPGTGLARREEYRAKRYHRPNDELDPAWELTGTAQLA
                     RFVELAIEEIGARGGKVPWRSGSPYAR"
     gene            27160..28887
                     /locus_tag="PLP50_05765"
     CDS             27160..28887
                     /locus_tag="PLP50_05765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA51093.1"
                     /translation="MSDTPERPVDGEEAEQSGFVSLGLDSRLLAALAALGYEEPTPVQ
                     RQAIPILLEGRDLLAQAATGTGKTAAFALPMLDRLVREGGERRGVFGLVLVPTRELAM
                     QVAEAVHRYGKQAGVRVLAVYGGSSMGLQLRALERGVDVVVATPGRALDHVRRGTLAL
                     ADVKLVVLDEADEMLDMGFAEDLEAILDATPDGRPMALFSATFPPRIAVLAARRLSSP
                     TELKLGASRAMGAAPEGQGRVKQTAYVVPRAHKLAALSRVLDLEDPTSAIVFCRTRTE
                     VDEISEKLNARGRRAEALHGGFSQEQRDRVMRRFRSGAADLLVATDVAARGLDVEQVS
                     HVFNFDVPSEAEAYVHRIGRTGRAGRAGSAITFYEPREQWLLANVERLTKKKIERATV
                     PTVADLKARRLELTRASLRETILGGGLDTFRVAVESLADEFDLLDVAAAGVKLAHEAA
                     GSPGEDEEIPAIPVPPERPRFAPRGDGPAPGPFPARRGPRLAGAVRLFVGAGRDAGIR
                     PADLFGAITNEAKLPSRAIGAIEIADRFSLVEVPGESADAVIEALRRTTLRGRKVLVR
                     RERPAPPRR"
     gene            29152..31176
                     /locus_tag="PLP50_05770"
     CDS             29152..31176
                     /locus_tag="PLP50_05770"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPA51094.1"
                     /translation="MTTTADSGPGSLRQAVADANASTGPDTIAFSGLAWPATISLVAP
                     RMDVVGDLTIDGPGPKLLTIEGDGTITGLFRLNRSADVQNRLVLRGATVRSFRFLYGG
                     AIEGYGATGPEPLESVIEVDGCVFEENGWASGDPNAQGGAISASGALTIRRSEFVGNT
                     TGGNGGGAVQSSGSLLVEDSLFWGNSAESDGGGAIRILGSGAVTRRIVNSTFSDNHVT
                     VDSANNDGGGAIAFVGLGAGTGLQVYSCTFAGNTTVQGGGGAILFTSQAAPAFSSLTI
                     SSSVFAGNTSGGSPNDLSPAADASVTADYSLFQAHASAAAAGIDSTVSSLFGVDPLLA
                     SIVDNGGPTRTRRPGIGSPAIDAGTNALGLSWDQRGAGFPRVVGGAADVGAHETGTTD
                     LFALTVTRTGPGAGAVASADFGIACGSECTDVYGAGTVVSLTALPEDGSVFAGWSGGG
                     CSGTGACAVPMNATQSVSARFEIGPAPGGETLTITIVGPGSVTSVPAGIDCAPTCSFS
                     FAAGAQVTLSPAPAADWAGTSGDPCVPGAATCTVTMADARNVTFYFLDSAASPVALTV
                     TRSGSGTGSVSSDLPGIACGADCAESYTKNSLVTLTAAPQAGSMFAGWSGGPCSGTAP
                     TCVVPMTEARTANAAFALLEPASVPGLGAFGGVLLASLLAAAGALAIRLR"
     gene            31342..31623
                     /locus_tag="PLP50_05775"
     CDS             31342..31623
                     /locus_tag="PLP50_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HPA51095.1"
                     /translation="MATKKEKPLAERFAEAKGRVEQLTKRPTNDQLLELYACYKQATE
                     GDVAGSRPGLLDLKGRAKFDAWAKVKGTGKDEAMKKYVALVDALRAELG"
     gene            31625..32278
                     /locus_tag="PLP50_05780"
     CDS             31625..32278
                     /locus_tag="PLP50_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPA51096.1"
                     /translation="MIFLDELRAEHVLIDRVAASLRSYAAARARGEGDASDGAAFRRF
                     FRLWAGAWHHAREEETLFPALAERAELSLSRGPLAAFVEQHHDMAAQIGAFEPLLDRA
                     VLTEEEGRRLVALVGAYTTDLGRHIDAENSVLIPESGVRLAQVNVLELHGRTPSAEEL
                     AARAEGERLAAAWPAAPDPGVRGEGCVVCPSFGTTCRGVEAEWWNESEWEEFSDHLG"
     gene            32478..33068
                     /locus_tag="PLP50_05785"
     CDS             32478..33068
                     /locus_tag="PLP50_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51097.1"
                     /translation="MASETRSYLAVSKEAVAWVKAMVPFGASNRPSDYVESWKKNRKK
                     IEGPDPGVLDKVLGGMTAFLPLLTGANRAMQIGNEVSGNAPSDYVSRFRQISEITLRT
                     KAGNCNEQSITAFVWLLDRGVRPLAWMHLTNGKHAFVVVGRKPGSGADPTKWGDGCVV
                     CDPWAGEAYSLPAVQAGAILQTKWGCGTANAEFGVA"
     gene            complement(33072..34799)
                     /locus_tag="PLP50_05790"
     CDS             complement(33072..34799)
                     /locus_tag="PLP50_05790"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPA51098.1"
                     /translation="MNAELIARARSWRDADPDPATVLEVDHLLEAGDEAALEDRFRSE
                     LEFGTAGLRGLLGAGPNRMNRAVVRRATAGVARHLLATVPDAARRGVVLARDARRGSE
                     EFALEAAGVLAGHGVRVFFFVDRAPTPLGAFAVKELGAAAGVVVTASHNPPEYNGYKV
                     YWENGAQIIPPVDAGIAGEIAKAGPAGALPFVPRAEAEARGLFVPLGEEMAERYHAEI
                     ARLALHPGEGRDLRIVYTAMHGVGARFTLAALAKAGFASVAPVAEQNEPDGAFPTVAF
                     PNPEEKGALDLALALCEETRADLLLANDPDADRLAAGARDRQGRMRVFSGNEIGVLLG
                     AYCLKEPRPKPEKPLVVTTIVSSSQLGKVARDLGAAYAETLTGFKWIENRALELERTE
                     ERTTVFGYEEALGYSVGDVVRDKDGISAALVFADLAGWCRARSITVWDFLEEIQREHG
                     LFLSAQKSVTIPGAEGAATIAAIMDGFRARPPAAIGGLAVAETRDYLAGVPGLPKSNV
                     VAYLLAGGSRVTLRPSGTEPKIKYYFELVESPRAGEPIPSAKSRGEKRLAELLDDFVR
                     LAVERGQPA"
     gene            complement(34825..36642)
                     /locus_tag="PLP50_05795"
     CDS             complement(34825..36642)
                     /locus_tag="PLP50_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA51099.1"
                     /translation="MPIRPGRRALLPCLLLSAFAAHGAPAAAQAAAPQAPPRTTASVE
                     LSLTNLDVVVTDAKGDPVRGLVAADFEVLHDGRPVELTNFSEIRLEGAAAVVPAITAA
                     PAGTTAAAAPPAATPGARPRRRVVLFFDRLYLPEPGVRKALFDALRSLVADTLDDGDD
                     VMIVTWERSVRTVLPFTDDLDALEPTLERIERRCARPGPEAGESALLEWETSWFKSQS
                     ADPRVGTAAGDGSETTMEILARQAYFEMKGKTAALKGLAATLGGVDGRKALVLVTNRF
                     SRFPGLEFLIGSRPDAGALEDAKRRAQDARKLLEEVTQAANANGVTLYGLFPDAFEGK
                     GLPSAADSPTSSPAWEHPNFDFSDIGGGRGAPAAPGRALPPASGPALGVRRDALLQNE
                     VEALDVVASATGGVALAGAGCVGRLVERVSRDLQSWYSLGFPSRPGTGRTASISVRVK
                     GRDLTVRARRAIVEKSVEEQMSDRVLAHLFQPDERSRIPISASLVGTTPGKDKHVLRL
                     EVRIPIESLALLPTAKGVQGVFSVFVASVAPEGDFSTVTRLNQPFEIPAKDLEKAQAG
                     HYTYELDVVTTGPGARVAIGIWDEKSNEAGFAIVTPPRT"
     gene            complement(36675..38351)
                     /locus_tag="PLP50_05800"
     CDS             complement(36675..38351)
                     /locus_tag="PLP50_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA51100.1"
                     /translation="MNGFRASLTPLLFSAAVLNVALPAAQAAPPTPPRATASVELSLT
                     NLDVVVTDAKGNPVHGLVAADFEVLHDGRPVEVTNFSEFRLEGGTALEAASPTATEPS
                     AAPAKPPRRRVVLFFDRLYLPEPGIRKNLFDSVRSLVAETLDDGDDVMIVTWERSIRT
                     VLPFTDDPGALRRALGRIEERCARPGREETDYINLDQEQAWFESLKEDPRFEGIPGDS
                     ELTAELTARQSYFEMQAKTAALQGLAATLGGADGRKVLVLVSHRYSRYPGLEFLIRSA
                     TDIEQILASKRKLQDARKLLDAVSNAANANGVTLYGLFPDAFEGLDVVSAGRSGGPPQ
                     GIVKDTLLQNEIEALDIVASATGGVVLAGGGNVGRLVERVSRDLQSWYSLGYPTRPGT
                     GRAASVSVRVKGRDLTVRTRRAIVEKSVEEQMSDRVLAHLFQPDERSRIPISASLVGS
                     RPEKGKNVLRVEVQIPIGSLALLPTAKGVGGAFSVFVAAVAPEGDFSTVNRLNQPFEI
                     PETELEKAKAGHYTYELDVVTKSPEARIAIGIWDEKTNEAGFALVTPSRT"
     gene            38421..39749
                     /locus_tag="PLP50_05805"
     CDS             38421..39749
                     /locus_tag="PLP50_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51101.1"
                     /translation="MISFVSLFVGFVVGIVNVQLMATAEVDRVELFLDGQVVAELREP
                     FSRPVDLGCEPAPHELVAVAYDERGRVLSSARQWVNRPRATADLSLLVDGGGDSSPRT
                     ARLVWRALAGEVPRSVTVTFDGAVLPAPDPQRIELPEHDPAHVHLLRADIDFGRGVVA
                     SAEMIVGGPSRVEARSELSAIPLSIEEGAALPAPEGLAGWLEAGGLPLEIAAVEDGPV
                     DVVVVGERSVPEALKRFGRRSRRSRRSRSELPATAPAVPSEAPAPVALDVRLRFLWPV
                     ATIWTQSAMVANNFLASSSSDEARLDLAWLTARHGDWPNWSGTSERLADAVAVAALTA
                     AEGNRRRAVVLVLGPSAKDDSLVTAEETARFLGRIGVPLHVWSISETPSPEASRWPAP
                     RTIASAADLDDAVRDLTAAAARQRIVWVEGSHLPQDVELGPLAKGIRLAR"
     gene            39876..41240
                     /locus_tag="PLP50_05810"
     CDS             39876..41240
                     /locus_tag="PLP50_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51102.1"
                     /translation="MVSFASLFVGFVIGVVNVELMASGGVDRVSLLLDGRPVAELREP
                     FRAAVDLGCDPAPHELVAVAWDAAGKELGRARQLINLPRPTAEASLVLEAGRGGTGRV
                     ARLTWRALVGEVPKALSVTFDGEPLPIADPRRIELPPHEPAQVHFLRAELEFGEGVSA
                     SAELIFGGSKRSEALADLTAIPVELEGEASPPSAAEMTGWLEARGVPLEVEGVEAAGV
                     DVVFVVEGSAIRQLRRAASRGADEYRRSSDSFLDPRKSPSGTPTFRRWGGTGGRRLSV
                     PVDLRFRIQRPVARMTAQKEMVANLFPSTRWFAFRALTPGRLVYALLSLSEEPPIGQR
                     LADAVVVAGLEAAKGNGRRAVVLILGPGAKDEGLLGAEDARSFLARLGVPLQVWSAGR
                     KHSREAARWKAQRLMSDSWEVEGAVHELLEAALRQRIVWVEGSHLPQDVELGPLAKRI
                     RLAR"
     gene            41274..>42543
                     /locus_tag="PLP50_05815"
     CDS             41274..>42543
                     /locus_tag="PLP50_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51103.1"
                     /translation="MVSFATLVFGLVFGIVDIEVRAPEATERVEILLNGESVAQLRRP
                     FSGAVDLGAVPIPGELVAIATDAEGRELGRCSQRINAPRANARARLVREPDDEKGRAV
                     ARLTWSCPTDAPPGSVDVTLDGQTIPAADPDRIVVPATDARGPRLLRAVVECGREVRA
                     VAEIGIGRDLPDIPSERTAVAVELDAGATLPAVDGLGGWFATESGPLRASAVDEVGLD
                     VVFVCEPSAFSELRRVFSKRAGPSRSTAREDLQFRFLLPAGGGAPGSNVLDWGRNGFA
                     ASKWFPRKWKAVGPTLATGIEVGDFFAPGQRISDAVALAGFAAAGRERRRAVVLLLGP
                     AAQDVSELSAAAAAQFIAALNVPLFAWSVGPSSSPIEEMWSVRRRVSTAAEFEAALAD
                     LREDLLRQRIVWVEGSHLPQDVELGPLAKGI"
BASE COUNT         5807 a        14751 c        16015 g         5970 t
ORIGIN      
        1 cggggctcga gggcttcaag aaggagttcg cgcgcgaccg cgccgagctc gcctcgtgga
       61 aggtcgccga cccgggccgc atctcgctcg aggagacgat cgagaacgcg aagccgtcga
      121 tcctgatcgg agtctcggcg accccgggga cgttcaccga ggcgatcgtg aggaagatgg
      181 ccgcgctcaa ccagcggccg ctcgtcttcc cgatctcgaa cccgacgtcg aaggccgagt
      241 gcacggccga gcaggccgtc acctggtcgg acggccgggc catcgtggcg accgggagcc
      301 cgttcgcgcc ggtcgtccac aacggggtga cgcaccggat cggccagtgc aacaacgctt
      361 tcatcttccc ggggatcggc ctcggcgtct gcgtctccaa ggcgcggcgc gtcagcgacg
      421 ggatgttcct cgcggcggcc caggccctgg cgacgcacgt caccgaggcg gacatcgccg
      481 agtcggccgt ctacccccag gtcgccacga tccgcgactg ctcgcactcg gtcgcgtgcg
      541 cggtgatccg gagggcggtc gcggaggggc tcgccgagcc ggagatcctg aacggcctcg
      601 aggagaccgt ccggcgcgcc atgtggttcc cggagtacct cccgatccgc tacgagccga
      661 cgggcctgcc cacccgccgg cacgcgcacg cctgatccgg cccgagggcg cgccgcgcgg
      721 tgcggcgcgc ccttgacgga agaccgctcc cgacggcccg gcgagagccg ggccgtcgcg
      781 tctcaggcct tcttcggcag gttcgaggct gcgaactccc agttcaggag gccgtcgaca
      841 accgcggtca ggtagtcctt ccggcggttc tggtagtcga ggtagtaggc gtgctcccag
      901 acgtcgagga cgaggagcgg cgtctgtccg gtcgtcagcg gcgtgtcggc gttcgccgtc
      961 ttcacgacct tcagcgccgc gccgtcggcg acgagccagg cccagccgct cccgaactgg
     1021 ccgaggccgg cggcgacgag ctccttcttc agggtgtcga ggctcccgaa ggaggcctcg
     1081 atgcgctcgg cgagggcgcc cgacggggcg gcgcccccct tcggccggag gctcctccag
     1141 aagaagtcgt ggttccacgc ctgcgcggag ttgttgaaga cggctcgagc cgaggcgtcc
     1201 tgtgccgagg cgcggacgat ctccgcgagg ggcttgccct cgagcgggcc tcccttcacg
     1261 aggccgttca gggcgtcgac gtagccgcgg tggtgctttc cccagtggaa cgagagcgtc
     1321 ttcgcggaga tgaccggctc gagggccgtg tcgggccagg ggagcggcgc gagcgcgagc
     1381 gcctcggctc ccttcaggat cgcggggaag ccgagcgcgc cagcggcgac ggcggtcgcg
     1441 gcggccccct tcaggaagtc gcggcgcgag gcgtcggtcg tttcgggacg gggcgtctct
     1501 tcgggcatcg tgtacctcct ggtggtcgtc ggggcggagg atacccggcg gtgtcccttc
     1561 cgcctcaggg gacgaggacg gtcgagccgg tcgtgcgacg ggcctcgagc tctcggtgcg
     1621 cggcggcggc ctcccggagc gggaacgtcg cgccgatccg gacggacacc gcgccggaac
     1681 ggatcacgga gaagaggtcc gcggcggtct cgaggaggtc ctcgcgccgc gccgtgtagg
     1741 ccatgagggt cggccgcgtc aggaagagcg accccttctg cgagaggagg aggggcgcga
     1801 acggcgggac cgggccggag gagttcccga acgagaccat gagaccgagc ggagcgaggc
     1861 agtcgagcga cgcctcgaac gtctcacgtc cgacggagtc gtagacgacg cgcacgccga
     1921 cccccccggt gatctcgtcg acccgctgca cgaggtcctc ccgagacgtc acgacgacgt
     1981 ggtgacagcc cgccgcgcgc gcgagctcgg ccttctcgtc cgtcgagacg gtgccgatga
     2041 cggtcgcgcc gaggcttcgc gcccactgcg cggcgatgag gccgacaccc ccggccgccg
     2101 agtggagcag gatcgtctcg cccgcttcga cgctgtgcgt cttccggagg aggtagcgcg
     2161 ccgtcatccc tttgagcatc gcggcggccg ccacgcggtc gtcgacgtcg tcgggtaggg
     2221 ggacgaggcg gtgggcggcg acgagccgct cctcggagta ggcgccgacg ggcccggcgt
     2281 aggcgacgcg gtcaccggcc ttcacctcgg tgacgtccgg gccgacctcg gtgacgacgc
     2341 ccgctccctc cgagccgagg accgcgggga gcgggagcgg gtagaggccg ctccgatggt
     2401 acgtgtcgat gaagttgagg ccgatcgcgg tgtggcggac gcgggcctca cccgggcccg
     2461 gcgggccgac ccggacctcg tcccacgaaa gggcctcggg tccgcccggt ctgtgaatgc
     2521 gaacggcgtg cgtcatgcgg cacctcgcgc accagcgtat cgcgacgggt ggacccgcgg
     2581 ggcgctcggc cgggctagac tttcggcgat ggcgctcccc atcgcccgac gggcgtggct
     2641 ggccgcggcc ctcgcgctcc tgctcgcgga cacgcgagca ctttctctct cgccggagag
     2701 gcagctgaac cagtacgcgg tctcgacctg gcagaccgcc gacgggctcc cgatgaactc
     2761 ggtcctcgcg atgactcagg cgggcgacgg gtcgctctgg atcgggacgg aggaggggct
     2821 cgtccgcttc gacggagccg agttcgaggt gcgcgaccgg ggcaatacgc cctcgatgga
     2881 cgcccacaac gtgcaggccc tctgcaccga cgaggccggg gccgtctggg cggggaccta
     2941 cggagccggc ctccttcgct gggacggccg ggccttctcg cggctggcgg cggagtcgga
     3001 gccggaaagg agccgtccga ttcgggcgct cgcgcgcggc gatgacggct cgatctggat
     3061 cgggacagcc ggcggtctcg agcggctcgt cgacgaccgc cggacgcggt tcgcggcgga
     3121 cccggggctc gcttcgtcgg ccgtcctcgc gatctcgccc ggtcggaagg gccgcgtctg
     3181 ggtggcgacg gtcgcgtggc tcggtttcgt ggagaacgga gcgctccacc gcctcgtccc
     3241 ggaacccgcg ccgggcgggg tcgtgctctc tctcgccgaa ggtccggacg gagcgctcta
     3301 cgtcgggacc gaccgggggc tcttccggat ctcgaccgac caccgcgcct gcgagcgcat
     3361 ccgagggctt tcgaacgact tcgtcagcgc gctcgccgtg gaccagaacg gcgcgctctg
     3421 ggtcgggacg aacggcggac tccaccggat cgggcgggac ggccggatcg agcggctgac
     3481 gacgggggac ggcctctcga gcgacatgat ccagacgctc ctcctggatc gcgagggcaa
     3541 cctctggttc ggcacgcggg gcggcggcct cggacggctg cgcgacggcg acttcgtcac
     3601 cttcggcggt gcgcgggaag gcttcgtcgt cgacacggtc ctctcagcga ggtccggcgg
     3661 agttttcgtg ggcggctgga acggcttcct ggcccgcctc ggacccgacg gccgtttcga
     3721 gcctctcgcg tctccggcgg cgctcgcggg cagctcgatc cgggccctct tcgaagaagc
     3781 cgacgggacg ctgctcgtcg gcgcgtggaa cgggctctac cgcgcgcgcg acggccacgt
     3841 ggagcgtgtt cccgaccgag gcctgatccg gaacgtgagg accgtccacg tcgaccagga
     3901 gggcgcgatc tgggtcggcc tcgaggcgga ggggctggtt cgactccagg cagggaaggt
     3961 ccggcgcttc acgacggcgg acggcctcgg gggaaatgag gtgcgcgccc ttctcgagga
     4021 ccgcgacggg acgctctggg tcgcgaccta cggaggcctg agccgccgga ccggcgagag
     4081 cttcacgacg ctcacgacgc gggacggtct ccgaagcgac ctcgtcaggt cccttgccct
     4141 cgacgcggac ggtgccatct ggatcgggac ctacggcggg gggatcgctc ggctcgaccg
     4201 gggcgtgatc acgtcctggg acacccgcga cggtctctac accgacgtgg cgtacgcgat
     4261 caccgaggac gacgccgggg atttctggat cagcagcaac cgtggtgtct accgcgtcgc
     4321 ccgggccgac ctcgaggcgc gggcgagcgg cggcggcccg aggctacgct gccggtcgtt
     4381 cgggatcgca gacgggatgg cgagcgagga gtgcaacggc ggggacccgg cggtggcgcg
     4441 gaccgccgat ggccgcatct acttcccgac ggtgcgcggc gtcgtcgcgg tcgctccgac
     4501 gcggctcctt cccacggccc cgccgcgggt gcggatcgat tccgtcgtgg ccggggcgat
     4561 cgcggtcgac catcgggaga cggccgaggt cccggccgga acgcgccgcc tcgagctccg
     4621 ctgggcggcg ctcgggttcc tcccgccgct caagaccgag ttccgctacc gcctcgacgg
     4681 attcgacgac gagtgggtcg aggccgggac gcggcgcgtc gcgacctaca cgaaccttcc
     4741 gccggggagc tactcgtttc gagtcgttgc cggactcgac gggagcttcg gcagcgcgga
     4801 gacgcggctc cccgtccggg tcgggcggct ctggtggcag acctggtggg cgcgggcggc
     4861 tctgggattc gctctggccg gactcctcgg cggcgcgtat cggctgagga cgggaagcct
     4921 cgcgcgcgat cgacggcggc tcgaagaggc cgtcgccgcg cggagcgccg agctcgccga
     4981 agcgaaccgg gcgctcgagg aagcggtcgt tacagaccct ctcacgaagg cgaggaatcg
     5041 ccgattcctg gacatggcgc tcgactccga gagggagctc gcggtccgct cctcggagag
     5101 caaggactcc tgcaggaacc gcgacctgat cctctacctc gtggacctcg acaggttcaa
     5161 gtcggtgaac gaccgtcacg gacaccggac gggcgacctc gtcctcgtgg agacggcgcg
     5221 gcgcctccag ggggctctcc gccggaccga cttcctcgtc cgatggggcg gcgaggagtt
     5281 cctcgtcgtg gcccgcggct cggagcgccg ccaggcggac gtgcaggctc ggcgtctgct
     5341 ggaggccgtg ggcgggtctc cgtactcgct cggcgacctc tccctcgtgc agacctgctc
     5401 gatcggctgg gcgcccttcc cgtggctcga ggagaggccg cgcgacgcga cgatcgagga
     5461 ggtcctggga ttcgccgacc gggcgcttta cgaggcaaag cggtcgggac ggaacggcgc
     5521 ggtcggctac gtcgccgggg gcgacgtcgg ccagaagggt tccggcgagt cgttcgagga
     5581 gcagcccggg cgcgtcgtcg aggtggtccg gacggagggt ccccggaccg gctgagagcg
     5641 cccgggcggc gtgttcgtcg tcagtcgcag gtgtggccgg ctcccttggc gggagcggcg
     5701 tgggcggccg ggccggcgtg ctgggcgtgg acggcctcca cgatcgcctt cgtgtagtgc
     5761 atcagctcga cgtaggcggc gacgtaggcg cggccgtccg ccggcgacgc gtctgccgac
     5821 ttcttcgccg cgagggcctt cgagaaccgt ccctggatgc cggccgccgc gtgctcgccg
     5881 acgagcttcg agaaggcgtc gaccgagccg gaggtgagcg cggcctccag ctgtccgatg
     5941 aacgcaggct ccttcacgcc ccgcttcagg cctgtgtagg cggccccctc ggtctcgcgg
     6001 tggagcctca cgagcgtctc gaagaaggcg gtgtcggcga cctccttcgc ctcggcgccg
     6061 agcttcctca cgccgagcgt cttccggaac gcctcgcgga tcgcgggctc ctgcgccgcc
     6121 gagatccact tcaggacggg cgcgacgtcg cccttctcga gcgcggcctt cgcgtcgacg
     6181 acgaccgggc cctcgacgga gtcgcagtgc gccgcggcgg gcgtcgcggg gaagaggagg
     6241 gccgcggcga gggtcgcggc ggcgaaggcc gggagcgtgg tccatttcat ctcgtcactc
     6301 cttcgggccg ttcggcccgt ttgaacgggg tttcggggaa acgcccgcgc cgcgcgggag
     6361 cgcgacgcgg gccggtatcc ggaggcttcg ggccgcgtcc gaactacgcg ctctccgggg
     6421 ccgtgtcgtc gtcgaagccc ggctcctccg ctttctcgta gctccagccg gtcaggaggc
     6481 cgacgaagaa ccagaggagg aacgcgaccc cgccgaggaa gacggtgtcg ccgaggatcc
     6541 ggagccagcg gagcgcctgg agggccggga gctggaggaa gtcggcgctc cgggcgtacc
     6601 agaggccgtg ctcgacgctc gcccaggtct gcgcgaggcc gatcgggagg agcgagagga
     6661 cgaccatcag gccgagtccg acgttcatcc cccagaacgc ccacttcagc tgcgtctcct
     6721 tccacgcgcg gacgccggag agcttgcgcg cgatgatcag gacgaggccg agcgagagga
     6781 ggccgtagac gccgaagagg gcggtgtggg cgtggaccgg cgtcgtgttg agcccctgca
     6841 tgtagtagag ggcgagcggc gggttgatga ggaagccgaa gacgcccgcg ccgacgaggt
     6901 tccagaacgc gacgccgacg aagaagacga tcggccagcg gtacttcgcc atccaggggg
     6961 cggcgtgctg gagcttgtag gtccggaagg cctcgaggcc gatgagcgtc agcgggacga
     7021 cctcgagcgc ggagaagctc gctccgaccg cgaggatcga gaccggcgtg ccgctgaagt
     7081 agaggtggtg gaacgtcccg ggaatgcccc cgaggaggaa gagggccgag gaggcgatga
     7141 cggccgcgcc cgcgtgcttc ttgtcgacga ggccgagctt cgcgaagagg agcgcgatcg
     7201 ccgccgtggc gaagacctcg aagaagccct cgacccagag gtggacgacc caccagcgcc
     7261 agtactccat gacggagagg tgcgtcttgg cggtgaagaa gaacccggcg ccgtagagga
     7321 ggccgatggc cgtcgtcgcg cccgtgaaca tcaggacgag cgatcgggcg tcgtcgcggc
     7381 ggcgcgcggc cgggaggatc gcgcggagca tcaggaccag ccagaggacg aggccgacga
     7441 agagggcgat ctgccagacg cggccgaggt cgacgtactc gtacccctgg tggccgagcc
     7501 agaacgagag gtcgagcgga aggacctggt ggatcgcgag gtactcgccc gcgagcgagc
     7561 cgacgacgac gaggaggagc gcgccgaaga ggacgttcac gccgaggcgc tggtacttcg
     7621 gctcgtgccc gccgatgacc ggcgcgagga agaggccggc ggcgaggaag gcggtcgcga
     7681 tccagaagac ggcggtctgg acgtgccacg tccgcgcgag cgcgtagggg acccactcgc
     7741 cgacggggag gccgtagagc gcctgccctt cgacggtgta gtgggcgagg agcgcgccga
     7801 ggccgacctg gacggcgaag aggccgagga cggcgacgac gtacttgccg acggcgcgca
     7861 tcgagggcgt cagcgtgagg ccgacgagcg ggtcgacggg gggagcggtg acggcgggct
     7921 cgggcttctc gcggaacgcg gtccaccaga cgacggcgcc gatcccggcg aggaggaggg
     7981 cgatcgagac gagcgaccag acgacgttcg cgcccgacgg gacgttctcg acgagcggct
     8041 catgcggcca gttgttcgtg taggtgatcg cctggccggg gcgctcggtc gtcgcggccc
     8101 agctcgtcca gaagaagaat gccgtgagcg ccttgcggcg ggccgcgtcg gggacggtcg
     8161 tctccgacat ggcgtagtcc tcgcggagcg gcttcagatc cggcgagccg ccgaagaggg
     8221 cgtcgaagtg agccgccgcg cgccgatacg cctcggcccg ctcggcggag atggtcacga
     8281 cgcctgtcgc ggcgtcgtgc gtgttcgccc gcatctcgcg gacgagggcc gccttcagcg
     8341 cggcctgccg gtcaaaggag agggaggcga agtcctgcgc tccctcggct tccgccttca
     8401 cgtcgaggag ggcggtcgcc tcgcggtgga ggtggtcggc cgtccagtcg ggggcctggt
     8461 aggcgccgtg cccccagatc gagccgagct gctggccgcc cgtcgactgc cagacctgct
     8521 ggcccgtcag gatgtcgtcc ttcgtcatga gggtcgcgcc gtcgggggtg acgacgcgct
     8581 ccgggatcgg cggggcctgc cggtagacct cgcggccgaa gaagccgagg acgaggaacg
     8641 tgaagaggag gactccgccg aggagcgccc aacggcggtt gtcgttgccg gggaggttcg
     8701 tgctcatcgt gccctttcga ttcacatgtc cgcgcagagc gcgcggcgga agagaatgtt
     8761 gttctcgagg tggacgtgct ccatcagctc ggcctcgagc tcgtcgaggc ggagatagag
     8821 ggcccgccac gtcgtgcagg cctcgatcgg cgggacgagg tcggtggtca gcgcgcgggt
     8881 cttctggagg gcgagggcga cgtcctcgtg ctcgcgctcg agcgactgga cctgcgccgc
     8941 ggcctggccc ccgcgcccgg agaggagcat cgggaagaga ccctgctcct ccttgtcgag
     9001 gtggtcgatc agctcctgcg agagagactc gacgtgggcc gcgagaccgc gggggacgct
     9061 ctccttctcg gcgtggacgc cttcgacctt ccgagccatc tggacgagga gagggagctc
     9121 cgtgcggagt cgcgcgtggt accagccgat gattctctcg acgatcgccg ggatcggctg
     9181 gtcggccatg gtctcgacgt cgccggaggt gcgggcctcg gcggcgacct cggagaggag
     9241 cgcggcggcg tcgaggccgc gctcggcgca ggcctcggag acgggacgac gcccgtgaca
     9301 gcagtagtcg aggccatggc gacgaaagac gcgggaggcg gccgggtagg cgacggcgag
     9361 gtcggcgagg gtcgattcag gctgaagcgg aaggttcatc ggtagcctcc gcatccctca
     9421 tcgcacgctt cgtgccgcac ttcacagcat ttggattcaa tgggttacgt gcctatgtta
     9481 tgaatgcgac agcagggagc gtcgtgaaga tcgcaacgtt gtcgtcgaat tcgcaacagg
     9541 aacctctcga ggtccttctc gagatcgtgg gcgacctgac ggcctcgctc gcggcgcgcg
     9601 accgatacga gcggctcctc tcggcggtcc gccgcctcgt cccctacgac gccgcctgcc
     9661 tccttcggct ccagggggac gacctcgtcc cgctcgcggg gcacgggctc gttccgggcg
     9721 cgctcgagcg tccctacccg aggtcccgtc atccccggct cgaggcgatc ctccgctccg
     9781 aagccccgct cctcttcccg cacgactcgc ccctgcccga cccgttcgac ggcctcctcg
     9841 tcgccgaccc gcacgccctc gaccggatcc acgcctgcct cggctgcgcg ctcaccgccg
     9901 acggagaggt cgtcggcgcg ctgacggcgg acgccctcga cccggaggcg ttcgacggcc
     9961 tcgacggtcg gctcctcgcg acgctcgggg cactggcggg cgcggccctc cggaccgcaa
    10021 gcctcatcga ggcgctcgag aggaaggccg agcgcgggac gcgcgtggcg cgagagctcc
    10081 agcggagcgc ggacgagacg agcggcgcgg cgatcctcgg ggcgagcgcg gcggtcgcgg
    10141 cgctgctctc ggagatcggc gtcgtcgccg cgtcggacct gccggtcctc gtgacggggg
    10201 agacgggcgt cggaaaggag ctcgtcgcgc ggcacgtcca ctcgggctcg cgccggaagg
    10261 acgaggcgct catccacgtc aactgcgcgg cgctccccga gtcgatcgcc gagagcgagc
    10321 tcttcggcca cgtgcagggc gcgttcactg gagcggtccg cgaccgcgcg gggaagttcg
    10381 aggtggccga cggcgggtcg ctcttcctcg acgaggtggg cgagctgccc ctctcgctcc
    10441 agccgaagct cctccgggcc ctccagcagg gggagatcca gcggatcggc tcggaccggc
    10501 cgcaccgcgt cgacgtgcgc gtcatcgccg ccacgaaccg cgacctcgcc gcggaggtgg
    10561 cgcgcgggcg gttccgcgcc gacctctacc accggctggc ggtctacccg atccgggtcc
    10621 cggcgcttcg cgagcggaga gaggacgtcc cgatcctcgc gacgcacttc gcggacgccg
    10681 cgcgccgccg cctcggcctc ggtccgctcc gcctctcgga ggaggcccgg gcggcgctcg
    10741 ccgccgcgga gtggccgggg aacgtccggg agctggagaa cgtcgtcagc cgggccgtcc
    10801 tgcgcgcggc ggccgggaag ccgcccggcg ccccggtcct cgtgcaggcg gcgcacgtcg
    10861 acgcctcctc gacggcgacg gcgccggagc gggcgccggc cgcgagcccg gacgccgcga
    10921 ggcccctccg cgagcaggtc gaggacttcc agcgcaggcg catccgcgag gcggtggagc
    10981 ggcatgcggg gaaccaggcc gccgccgcgc gcgagctcgg gatgcaccgc gcgaacctcc
    11041 accacctcgc acggcggctc ggcctcaggg gctgaggcac ggggacgccg ctccgggacc
    11101 gatcccgata tcctcggcaa tcccgatgga cacgagcttc gtctcctcca ccatccttct
    11161 cgtcctgatc acagacccgc tcgggaacat cccgttcttc atctccgcgc tgaggcacgt
    11221 ccgccccgag cggcgccgcg tcgtcgtcct ccgggaatgc ctcgtcgcgt tcgcggcgct
    11281 cctcctcttc ctcctcgcgg gacggccgat cctcaggctg ctccatctga gcgaggacgc
    11341 gctccggatc agcggcggcg tcgtcctgct cctgatcgcg atccggatga tcttcccgga
    11401 gcggggggcg cgcctcgggg aggaggaggg ggaggaccgc gagccgttca tcgtgccggt
    11461 ggcgatcccg ctgatcgcgg gtccctcggc gatggcgacg gtcctcctca tgtcgacgcc
    11521 ggacccgctc cggatgctct cgctcgccgg gtcgctgacg gtgacgatcg cgatcacggc
    11581 ggtcgtcttc gtctcgagca cgcggatcca gaaggtgctc ggggaccagg cgatcatggc
    11641 gatggagcgc ctgatggggc tcgtcctgac ggcgatcgcc gtcgagatgc tcctcggcgg
    11701 cgtcgcgagc tacatccgcc gcggtccggg ctgaggagtc ggacggggag cggagcgcgc
    11761 ggcaccggcc gacggggcgg gcgtcgggac ggaggcccac gggggcgcgg ccgccggcga
    11821 ggcctccgga gtgcggggcg tcagcctcgg gctccgggac ggcggaaccg gggcggctcg
    11881 cgcaggcgtc ggagccgggg acggcgtggc gcggccggcg ggcgcgggcg tcttcgtccg
    11941 ccggatggga agggctgagc ggagcccggc cgaagcgggg gcgccggacg cctcgggagg
    12001 cgtccggacc ctctctcgct ccgggtccac cggcagcccg ctccgaacga cgggagcgtt
    12061 ccggaggtcg cggaggagcg cggcgtcgtg aacgagatcg ctctcgccga agcggccgcg
    12121 ggcgaagatg gcgtgcggaa gatagagcgc gcggtcgcgg tggaggtacc ggaacgacga
    12181 gtcgccggtc ggcccctcca cccgaaccca ccagggctgg agaggctcgc ccggagcgag
    12241 agggaaccag ccgacgtagc cgtcgggtcc gagcggcccg acgcctcccg ggccggctcc
    12301 gacgaacgtg acgacggccg ggagaaagga cggaccgggg tccttcggct cgtccgggag
    12361 ccaggcccag cggctccgga cgatcgccca gcggccgtgg tggtacggag cccagcccca
    12421 gggctcgtcc gagagccagg cccagcccca cggctcgcgc cagatccagc ggcccgagcg
    12481 gtacggcatc caatccgcga ggctcacctt cgggaaccag gcccatcccc gctccgctgt
    12541 ctcctcccaa tcgccccacg cgtcgaggtc gtcgatgccg aggacgtcgg cgtggacgtg
    12601 ggccgctgag cggacgagcc ggaaacgggc tgagcgctgg tccacccggc ggtcccatgc
    12661 gtcaccccgg ccgagggtga cgacgtcgta ttcgggccgg ggcgaggcgg tcacgcgcat
    12721 ccgctcgccc cagccgagcg tgacgccccc ctccgccgtg gcggcccggg cccgacccct
    12781 ccggatgctg atcgccgtgc cgccggagtc gccgacgtcg aatcgatagt ccccagggag
    12841 ctcgagagcg acggagacgc tcggcgtcgc gacctccagc gagcccgcgc cgcgaacgtc
    12901 gaagacgcga acgctgaccg ttcccgcgga aagagtgacg gaccggtcct ccaacgagag
    12961 gtccggaaaa gcgagctccg tcccgggcgc cagatggatg accgcgcccc ggagctgcac
    13021 ctccgcgcgg ccgccggccg cgaccctgag ccggtcaccc gtcgtgagcg ggtcgttgac
    13081 ggccccgggc cgccacccct ccgctgcgtc gccgcgcgcg accgcgacgt ctccttcgag
    13141 ccaggcgagg cgcgccaccc ccagccggat cccgtcgtcc tccggcttcg aatcggacga
    13201 cgccccggca gacgcggagg gggcgaggag ggcgaggagg acgaggaacg gaacggaggc
    13261 gaggcggcgt cggacggaca tttggacctc actctctctc atccggcgga acggcggacg
    13321 cgacggcgga aacgggcccg gccgtcccgc ggagcgggct ctcaccgcgg ggcccgggac
    13381 ggcgtgctgc tacacggcga ccggcttctc cgccacgatc cgcgcgagga cggtcgagag
    13441 catgtcgacg tgctcgcgct cctcggcctc cagctcgcgg tagagcctcc actcgtgcga
    13501 gccggtcggg aacttccggc cggcgtcgag gaagaaggcg cgggccctct tctcgaggtg
    13561 gacggcgagg cggaggagcg cggctccggt gaggtcggcg atgtcggcgc cggcgtagac
    13621 cgcgatctgg gccggctcga ggtgcatgtc gagcgactcc gtgggcgcct cgacgtggta
    13681 gcgccgggcg aggacgtcca tgtgcccgcg ctccatctcg gcgaggtcgc ggaagagctt
    13741 cgcgaggtcg gggtccttcg cctcgagctc ctccgccccg cgcgagtaga acgtgacgcc
    13801 gccgagctcg atctcgaagg cgtggcggat cgccgcgacg gccgcctccg acgtgacgtc
    13861 ggtgtcgtgg ccgagcgaga gcgtcccgcc gcaggcgggg cagaggccgt aggggaaagc
    13921 gaagccctcg gagaccttct ggcaggaggt gcaggtccag gtgaccttct cgccgccgca
    13981 gcagaggtgg tcctcctcgc ccggctcgaa gggacgccgg cacttcgcgc aggcggccgc
    14041 gaccggagcg gggacgggca cgccggcgcc ggcggcctcg tcctcgagga cgacgggcca
    14101 ctccttcgtc ttcaggtagg tctcgatggc gcgcgccgcc cttcggccgg cgccgagggc
    14161 gaggatgacc gtggcgccgc cggtgacgat gtcgcctccg gcgaagacgc cggggaggtt
    14221 cgtcgcctgc gtcttcgcgt cggcgttgac gtacccctcg ccgcggaccc cgaggtccgg
    14281 cgcggagcgg gcgatgacgg gattcgcgcg cgtgccgagg gcgtagatga ccgtgtcgca
    14341 cgcgaacgtc tcgtgctcgc ccgtcgggac ggggcggcgc cggcccgact tgtcgggctc
    14401 gccgagctgc atcttttcga cgacgacggc cctcacgtcc cccttctcgt cgacctggat
    14461 ctccgtcggg ctcatcagcc agttgaagtg gatcccctcc tccttcgcgt ggcggatctc
    14521 ctcgacgcgg gccggggcct cggcctccgt gcgccggtag atgcagtgga cctcctcggc
    14581 cccgaggcgc cgcgcgacgc ggaggcagtc catcgcggtg ttcccggccc cgaagacggc
    14641 caccttcttc cccatggcga tcggcgtgtc ctcgaacggg aacttgttcc catgcatcag
    14701 gttgatgcgc gtcaggaact cgttcgcgct gtagacctgg cccgcgtgct cgcccgggat
    14761 gccgaggaac atcgggaagc cggcgcccgt gccgatgaag acggcgtcgt agcccatctc
    14821 gcccttgagc tggtcgatgg tgaaggtctt gccgatgacc ttgttcgtct cgaacttcac
    14881 gccgagcttc tcgagagcct tcacctcgcg gtcgatcgtg gtgcggggga gacggaacga
    14941 cgggatgccg tacttcagga cgccgccgac gacgtggagc gcctcgaaga cggtgacgga
    15001 cgctcccttg cgggcgaggt cgcccgcgca ggcgaggccg gccgggccgg agccgacgac
    15061 ggcgaccttc ccgagcgtcc cggcgttctc ggggggcttc acgtccggga gcggcgcgaa
    15121 gtcgccgacg aagcgctcga gccgcccgat ggcgaccggc tccatcttct tctccaggat
    15181 gcactggatc tcgcactgcg actcctgcgg gcagacgcgg ccgcagatcg acgggaagag
    15241 gttgctctcc tggatgacgg agagggcgcc ttcgaggtcg cggacgagga ggtgccggat
    15301 gaagcggggg atgtcgatcg agacggggca gcccgcaacg caggtgggcc gggcgcactg
    15361 gatgcagcgg tcggcctcct ggagcgcgtc ggccatcgtg tagccgaggt tgacctcctc
    15421 gaagttgcgc gagcgctcga gcgcgttcct ctcgggcatc ttcacggcgt gctcgtcgag
    15481 gtccttgaac ttcttgtagt tccgcttgtt catctcgaag agctgctcct cgatggagca
    15541 gaccttcgag aagtcgtcct tggcccgcga ctcctgcggc ttgaagcgct cctgccggag
    15601 ctgcagctcg gggaagtcga cgaggtggcc gtcgaagtcg gggccgtcga cgcaggcgaa
    15661 cttcgtctcg ccaccgacgg tcacgcggca ggagccgcac atgccggtgc cgtcgaccat
    15721 gatcgcgttc agcgagacca tcgtcttcac gccgaagggg cgcgagacct ccgagcaggc
    15781 ccgcatcatc gggagggggc cgatcgccac gacgaggtcg ggcttctcct tctcgaggac
    15841 ctcctggagc gccgcggtga cgaagccggg cttgccgtag gagccgtcgt cggtgcagac
    15901 gatcaggtcg tcgcagtagc gccggaagcg gtcttcccag aagacgaggt ccttgtttcg
    15961 gaagccgatg atcccggtcg tccgattccc ggcctccttg aaggcgcgca gctgggggaa
    16021 gacgggggcg accccgaggc cgccgccgac gaggacgacg tggccgacct tcccgacgtg
    16081 gctctcgagg ccgagcggcc cgacgaagtc ggcgaacgtg tcgccttcct tgtaggcccg
    16141 catcatctcc cgcgtcgtct tgccgagcgc ctggatgacg atcgtgaccg tcccccactc
    16201 cgggtcgtag tcggccacgg tgagggggat ccgctcgccc ccctcgtcca gccgaagcat
    16261 cacgaaatgg cccggccggg ccgccttcgc gacgtcggga gcgtggacgt cccagaggaa
    16321 ggtcacgtcg gagaactgct cgcgcttgac gatctggtac acgaatcccc cttgccgggg
    16381 cccggcggga cgctgcgacc gccgaacccg accgcggatg atgcccgaaa cggggcttcg
    16441 gcgctctcgg cggcggcctg aggagccgca ggacgctgca tcgcggcagg aagcggccgc
    16501 cggcgaggag ctcgcgggtg cccccgggcg gcccggagcc ggcccccgag gggtgagctc
    16561 ccgtcgacgg ccgggcccct cgcggtctac cggcggagcg tccgcccttg cgcgagggcc
    16621 gacgcgccgg cgggggcgtc ggagcggctc ttcctcacgc agaagcagga agtttcgagg
    16681 ctcgtgtcgg cgcgcgccca ggcgacggcg aaccgcgcct cgacggcctc cgcctcggcg
    16741 gacttcttct gcatctcgag cgccgtggcg aggccccgga gcgcccagcc gttcccgggg
    16801 taggcccgca ggtcctcgcg gtagaccgcc tccgcctccg cgggccgctt cgcgtcgagg
    16861 aggatcgcgc cgagcgcgtg gcgcgagggg accgtccagg ccggcggctc gtcgtacttc
    16921 agcgcgtcct cgagccggac cgcgcttcgg agcttctcga cggctcccgc gtgatctccc
    16981 agccgatagg cgatctcgcc gtcgaggtag gggaggccga ccgcgaggac gtcggccgcc
    17041 gggttcgacc cccagtagaa ctccttcggc accatcggga gcgccgcggc gaaggcggcc
    17101 cgctcccgct tggcctccgc gacctctccg agcgcggcgt gcgccacgcc gcgcaggaag
    17161 tgggcgtgcg ccgtcgagac cggaagccac tcgggaggcg ccttcgcggc gaggatctcc
    17221 ttccagaggc cgaaccgctt ctgcgcctcg ccgacgaccg tcaggaaagc gtcggcgaac
    17281 gccgcgttct ccttcacgaa cgccgggtcg agaccggcga cgacgcccgt cgctgtcgcg
    17341 agggcctcct ctcggcggcc ctccatcatg gcggtgtagg cgaagaagtg ggcgttgtgg
    17401 gccatgtaga tccggtagaa gccgatctcg ccgctgcggc cctggtacct ccgatcggcg
    17461 gccatggccc gctggttcgc ctcggcggcc tccttccagc ggccgacccg cacgtcgatg
    17521 tgggagggca tgtggacgag gtggctcgag tcggggacga gggcccggag gcggtcggcc
    17581 gcctcggcag cgcgctccgg ccggggcgag gcctcgagcg cgtgaatcgt caggtggagg
    17641 gccccggggt ggtcgggcgc gagcttccgc gcctcctcga gggcggcgag cacctcggcc
    17701 gtgccgggct ggggcttgcc gtccttcgtc cactggtccc aggggcgggt gtccatgagc
    17761 gcttccgccg cgagcgtcgc cacgtcggcg tcggcgggaa agcggtcccg cacggcgagc
    17821 atcgccttcg cgtaggccgc gtcgagcggc gcccggtcgg ccggcggcgg ccacgagtag
    17881 cgcgccccga gcgcctcgat cagggccttc tccacgtcgg tcgcccgccc cgcgagcgcg
    17941 cgggccttcg tgagcgcctc ccacgccgtc ctcgactgca cctcgctcat ggcggggttg
    18001 ttgatgtgcg gtccgttgac gagcgcgacg ccccaccagg ccatggcgag cgcggggtcg
    18061 aggcgggcgg cttccgagaa cgcgcgctgg gcctcgtcgt gattgaacgc gaaggcccag
    18121 acgagtccct ggtcgaaggc cttctgcgcc tcggcgactc gcgtcgagac gggacgggaa
    18181 tacgacccga gctcgaaggg acgccacgcc agcggcgggt cgacgtcctc gggcgtcgac
    18241 gaagcagccg ggccggagag ggcgagcgca gaaatggaga cgaccagcaa aacgaaccaa
    18301 tggaccgtcg agtgggacgg cctgttcagc ctcatcggga cccccaagcg agacgttttc
    18361 tcagaatctg gaccccgcgg tccgaaacgt caaccctacg ggccttcgat gggcatcgag
    18421 agaaggggcc ccgggcgacg ggtccggcac caacggccga tgcccatcgc gaggggcccc
    18481 tcgtggtcgt cagttctcaa gcggtcgcga ggaccatcga tcgggccatc gcgctccgcg
    18541 ccgcggcggc gatcgcgtcg gcctgcggga cgaaggcctg ctcgaggacc cagctcgaag
    18601 cggcgggcgc gtccgggccg gcgagcctca cgacgggcgc tttcaacgcg tcgaacgcct
    18661 tctcggcgac cgtcgcggcg atctccgcgc cgaagccggc cgtccggctc gcctcgtgga
    18721 cgacgacgag gcggccggtc ttcctcaccg aagcgaggac cgcctcctcg tcccacggct
    18781 tgatcgtccg aaggtcgatc acctccgcag agatcccgtc ggcggcgaga gtctcggcgg
    18841 ccgccaggca gccgaggacc gtcttcgcgt acgagacgag cgtcacgtcg gagccgggac
    18901 gccggatcgc ggccttcccg agcgggacga ggtgctcgcc ctcgggcacc tcgccggggg
    18961 cgtggaggag cgcgacgtcg aggaagaagg cgaccgggtt gtcgtcgcgg atcgacgact
    19021 tcaggagccc cttgaagtcc gccgcggtcg acggcatgac gaccttcagg ccgggcgagt
    19081 gggcgaacca ggcctccacg ttgtggttgt gctgggcgcc gacgccccag ccggcgcccg
    19141 tcatcgcgag ggcgaccatc gggaaggaga actgcccgcc ggagaggaag cggagcttgc
    19201 cggcgctgtt gacgagctcg tccatcgcaa acgtgaggaa cggggcgaag aggaggtcga
    19261 cgacggggcg gaggcccgtc gcggcggcgc cggcggcggt gccggcgatg atcccctcgg
    19321 cgaggggcgt gttgcggatg cgggcggcgc cgaactcggc gacgaggtcg tggcgcttcg
    19381 tggcgatccc ctcgccgaac gcgaggacgg tcgcatcgcg gcgcatctcc tcggcgaggg
    19441 cctctccgat ggcgtcgtag ggggtcatcg tcttcatggc gtgtccttct caggcgtaga
    19501 cgtgggtcgt gagctcgtcg agcgagggcc agggggagtc ggctgcgaac gagaaggcgg
    19561 cgtcgaggcg ctcggcgacg cgccgctcga tggcggtcgc ggcgtcggcg gtcagggccc
    19621 cgctctcgag gagcttcgcg cggagggcgg ctatcgggtc gcgcgccttc cacgacgcga
    19681 gctcggcctt gtcgacgtag ccctggtcgt cgggctcgaa gtggccgcgg aggcggtagg
    19741 tcttcgtctc gaggaggaag ggcatccgcg tggcgcggac tctcgcggcc gcctcggtcg
    19801 cggcggcgag gacggcctcg acgtcgttcc cgtcgaccgt ccgtccctcg atgccgtagg
    19861 cggaggcacg cgaggcgatg tcgtccgtga gcatcgtctc gcggcggtgg acgaaggcct
    19921 gccactcgtt gttctcgcag acgaagagga ccgggagctt ccagacggcg gcgaggttca
    19981 gcgcctcgtg gaagaccccc tcgcaggccg cgccgtcgcc gaagaagcag gtgacgatct
    20041 cgtcgccccc cttcatcgag accgagagcg ccacgccggg agccagggag agctcgccgc
    20101 cgacgatcgt cgacgtgagg atcacgccga gctccttcgc ggagacgtgg agcgtgccgc
    20161 tcttgccgcc gcagtagccc gtcgcccgcc ccatcacctc ggccatgagg cggcccgggt
    20221 cggcgccacg ggcgaggagg tgcccggcgc tccggtggtt cgtcaggatc aggtcgtcct
    20281 cccggagcgc ggccacgacg ccgacggcgg aggcctcctg gccgacgcag gtgcaggtgc
    20341 cgggggagcg cccctcggcg aaggaggcgg cgctccgctc ctcgaaggag cggatgagct
    20401 gcatcttctc gaggagctcg gcagggctcg gacgggtcgg gtccttcgac atcgttcacc
    20461 tctcgtacgg cgcggggcgc ctgtctcggc ggggcgttcc cggggggcgc tcccgacgcc
    20521 ggggaagatg cgacggtcgg aggcgtcccg ccacctaaga atcgcctaag caatcctaaa
    20581 aggtcgctat gcggaattcg cgcggggcga ggccgcgatc ggctgtcata ctggccgcgt
    20641 gaggcgcgcg gcggcactcc tcgcgctcgc cctcggggcg gcaccggcgg catgccggga
    20701 aggagtgaag cccgtccgga tcgccgtcca cagcgctcca cagagcttcg acccgcacct
    20761 gcagaacgag atcctcacgt cggcggtgct cgcgaacctc tacgacgggc tcaccgagtt
    20821 cgacgaggac tcccgggtcg tcccggcgct cgcctccgag tggcggaacc cggacgagaa
    20881 gacgtgggtc ttccgcctgc gcccgggcgt cgtcttccac gatggccggc ccctgacggc
    20941 ggcggacgtc ctcttcagtc tcgaccgagc ccgccaccac tccggcagcg gtctcgcgag
    21001 ctacctcgtg gagatcgagt cggtccggat ggccgacccg gagacgatcg agatccggac
    21061 gaggcggcct ttcgcggccc tcctcgcgaa gctcacctcg atcgcgatcg tccccgcgga
    21121 cgccccgccc gagatcctcg acccgatcgg caccggctcc taccgactgg ctcgagcgac
    21181 ggcggacgcc gtcgagctcg taccggcgac gaacggctgg cgccgtggcc cgctcctgcc
    21241 ggtgacgttc gtcgtggacg gcgacccgcg cggccgacgg gagcggctga tgaccggaga
    21301 cgtcgacgtc gccacggacc tcgccgaggt cgccgtgaag agactcgagg catccgagtg
    21361 ctgccgggtg gtcgcgcagc cgggctcgac ggtcgagtac ctgcgcctct ccccggcgcc
    21421 gcctccgttc cgggaccgtc gcgtgcggca ggcgatcgac ctcgcgatcg accgagccct
    21481 ctacgtcgcc gaggcgcacg gcggcatcgg ccagccggcg ggccagctct gcgtgccggg
    21541 ggtcttcggc tttgcgcccg acctgaaggc gacggcccgg gacctcccgc gtgcgcggcg
    21601 tcttctcgcc gaggccgggt tcggcgaagg cctcgacctg gtcctcgagc atcgtcccgg
    21661 ccggcgcggc gacatcctcg ccggccagct cgccgaggcc ggcattcggg tgacgccgct
    21721 cgtcacgccc tggacggagc tccacccccg gatccggagc ggcgcggtcg acttctattt
    21781 cgggggtgtc gtggcgcaga cggccgaggc gagcgacgtc ctggacagct tcgtccacac
    21841 ccgcgacgac tctcgcggct acggctcgac gaaccactcg cgctactcga accccacggc
    21901 cgacgccctg atcgagtcgg cctccgcggc cctcgacctc cggcgccgga gggacctgct
    21961 ccagcgggcg atgcgggtcg tgatggacga cctctacctc ctgccgatcg ccgggctgta
    22021 cgacatctac ggaatccgaa aggagatcgt gttcgagccg aggttcgacc gcaggcttcc
    22081 cggccgggga atcggccgga cccgatgagc cgggcgatcg ccgccgcgct cgccgtctgt
    22141 ctcgccgccg tcgccgcgtg cgaagggccg gggcgcccga tccgggtcgc gatccacagc
    22201 gcgccggtgg agctcgaccc ccacctgcag aacgagtcgt tgacgtcggg ggtcctcgcg
    22261 aacgtctacg agggtctcac ggagctcgac gaggagaacc gggtccgccc gaagctcgcc
    22321 tcgggatgga cgaaccccga cgagaggacc tgggtcttct cgctgagggc gggcgcgcga
    22381 ttccacgacg gacgggccct caccgcggga gacgtcgtct tcagcctcga gcgggcgcga
    22441 cgccacccgg gctccgctct cgcgagctac ctcgtcgagg tcgagtcggt ccgggtcgtc
    22501 gacgacggtc gggtggagat ccggacgcga cgaccgttcg cctcgctcct cgggaagctg
    22561 acaccggtct acgtcgtccc ggccgggtcg ccggagcgga tcaccgagcc ggacggaacg
    22621 ggcccctatc gcctcgtcca cgccgaccgg cggcggctgg agctcgtccc ggtcgcgacg
    22681 tccgcggagc gccccgaggc gcctggcccg ctccacttcg tcgtggagcc ggatcctgcc
    22741 cggaggctat cggcgctcct cgacggagag gtcgaggtgg ccgccgacct cggcgaggac
    22801 gcggtggacc tcctcgcgac gactgcctgc tgccgggcgg agctccttcc cgggtcgacg
    22861 atcgagtacc tccacctctc ggacctcgag ccgccgttcc gcgaccggcg cgtccgggag
    22921 gccgtccacc tcgccctcga ccgcaccctc tacctggccg aggcgcgcca cggcctcggc
    22981 cagccggccg gccagctcgc cgtgccggga ctcttcggct acgagccggg cctccgggcc
    23041 ccggaaaggg acctcgcgcg ggcccgcgcc ctcctcgagg ccgccggcta tgtggagggc
    23101 ttcgacgtcg acctcgagca tcggcccggc cggcgggccg acgtgctcgt gcggcagctg
    23161 ggcgaggccg ggatccgggc cacggcgcgc gagtcggcct gggcggagct ctatggaagg
    23221 ctccggaacg gggaggtggg cttctacttc ggaggcgtcg tgtctccccg acgggcgagg
    23281 cgagcgacgt cctggacggc tacgtccact cgcgcgagga ggaccgcgga tacggcgcct
    23341 cgaaccactc gaggtactcg aaccggacgg ccgacgggct gatcgaagcc gcgagctcct
    23401 cgttctcgct cgtccggcgg cgggagctcc tgcaggaggc gatgaaggtt gtcatggccg
    23461 accttcactt cgtcccggtg gcggggctct accaggtgta cggaacgagg agggacgtcg
    23521 agttccggcc gaggatcgac ctgaagctgc tcggagaccg gatccgcagg aggtaggccg
    23581 ggctccggcg cccgtcgccc ggccttcctc ggggtacgat ccgcgggatg gcagcccctt
    23641 cgacagcctc ctctccggct ctccgttcct cgtccgtcac ggcctcgccc gttgccgagg
    23701 tcgaggcccg ccatgtcgcc cacgtgctgg acgaaaggct cggcgagcgc ggagacggaa
    23761 cggccctccg ccatctcgtc ggcggccagt ggcaggacat ctcgtggcgc gagctcgccg
    23821 cgaaggtccg ggacctctcc tccgccctcg tcgaggcggg cgtcgcggag ggggaccgcg
    23881 tcgcgatcct ggccgcgaac cgccccgagt ggacgatggc cgacctcgcg atccttcgcg
    23941 tccggggcgt ctcggtcccg atccacgcga cgagcagctc gagccaggtc gccttcatcc
    24001 tgaaggactc gggagcgagg gtcgtcttcg tcgacgcggg ggagcccgcg tcgaaggtcg
    24061 tcgcggcgct cggggcgggc gttccgctcg atcgcgtcat cgtcttcgga gggggcgcgc
    24121 tccccgaagg ggcggccttc gcggatttcg aggggacgct cgcgcggggc cgcgccgccg
    24181 cgcgcgagga cgaagtcgcc gcgcggctct cgcgtgcgac gcccgacgac ctcctgacgc
    24241 tcctctacac ctcggggacg acgggcgagc cgaagggcgt gatgctcccg cactcggcct
    24301 tcaccgtcac gtgcacctat cacgacctcc ggctcccgcc gatcggtccg gacgacgtct
    24361 ccctctgctt ccttccgctc agccacatct tcgagcgggc gtggacgttc tacatcctct
    24421 atcgcggcgc cgtgacggcc tactgcgagg acccgaagaa ggtctccgag gtcctcccgc
    24481 aggtgaggcc gacgctgatg tgcgccgtcc cgcgcctcta cgagaaggcc tacacgaaga
    24541 tccaggagag ggtccggagc tcgtcggctc cgaagcgggc cctcttccgc tgggcgatcg
    24601 gggccggaca cgcggtgagc ctccggaggc gggccggtga ggcggtgccg cagctcctcg
    24661 ccctccgtca cgcgctcgcg gaccggctcg tcctcgcgaa gatccgggcc gcgttcggcg
    24721 gtcgcccgcg cctcttcccg tgcgccgggg cgccgctcgc tcccgagatc gaggagttct
    24781 tccactccgt cggcgtcttc gtctgccagg gctacggcct cacggagacg accgcgaccg
    24841 tgacgtgcca cgacccgtcg cggttccacg tcgggacggt cggctcgcca ctcccggggg
    24901 tcgaggtgcg gatctcggaa gagggggagg tcctcgtgaa ggggcggacc gtcatgaagg
    24961 ggtactggaa ccggcccgag gagacggcgg ccgtcctccg ggacggctgg ctgcggacgg
    25021 gggacgccgg cgagatctgc gaggacggga cgctgaagat cacggaccgg atcaaggacc
    25081 tgatcaagac ggcgggcggg aggtacgtcg ctccgcagca cgtcgagacg acgatcggcg
    25141 ccgacccgct cgtcgagcag gtcgccgtga tcggggaagg tcgcagctac gtcacggcgc
    25201 tcgtcgtgcc ggcattcgag gcgctcgaga agtgggcgcg ggccgccggg atctccttct
    25261 cggggcggga cgacctcgtg cggaacgagc gggtcctcga cctcctcaag cggacgatcg
    25321 aggagcggac gaaggacctc gcgccgtggg agaaggtgcg gaagttctcg ctcctgacga
    25381 aggagttctc cgtggaaggg ggctcgatga cgccgacgca gaaggtgcgc cgacgggccg
    25441 ccgccgagct ctacagggac cggatcgagg agatgtacac gggagactga ggcgggtcga
    25501 cggacgcccc gcccctatac tgggcgtccc gtgcgccgca tcgcattcgc cctcgccgtc
    25561 ggcctttcct tccccaccct gatcgttcgt cccgtccgag cagccgagcc cgcgcccgtc
    25621 tcgccagccg agctcgacgc gcatatccgc ttcctcgccg acgacctcct cgaggggcgc
    25681 gcccccggga cgcgcggcga cggcctggcg caggcctaca tcgagtcggt cttccgggcg
    25741 aacggcctcg cgccggcttt cggggcctcg tatcgccagc cggtcccgat ccggatcgtc
    25801 gtgcccgatt cgaagacgac gctgacgtgg acgggcgcgg cggccacggc cccggccggt
    25861 cggttcggcg acgacttcgt ccttgcgttt ccacgccccg agtcggccgg gagcgtctcg
    25921 gccgacgtcg tcttcggcgg ctacgggatc gaagcgaagg aatggggctg gaacgatttc
    25981 gaggggtccg acgtccgcgg gaagctgctc ctcctgctgt cgggcgagcc gggcggcgac
    26041 gacccggcgc tcttcgccgg cccgatcctg acgcatcacg gccggtggcg gacgaagctg
    26101 gaggtcgccg cgaaacgcgg cgccgcgggg gtcgtcttcg tccacacgcg cgagggggcc
    26161 ggctacggct gggaggtcgt ccggaacggc tggacgcgga ccgtcgcgtt cgcccccgat
    26221 gccacgaccc tctcgctcga gggctggctg acggaggcgg aggcgacgcg cgccgtgcgg
    26281 gcggggggcg tcacgctgga gtcgctccgc gcggccgccg ggaagcgggg attccggccg
    26341 attccgctcg ccctgaagct cgaggcgcac ggggcgcccc gctacgagtc ggtcgtctcg
    26401 gcgaacgtcg ccgggatcgt ccccggccgc gggccggcgg gaaccgctcc ggtcgtcgtc
    26461 gtcagcgccc atcacgacca cctcggcgtc gggacgcccg aggagggcga cggcatctac
    26521 aacggcgcga tggacaacgg ctcggcgctc gcggtgctcc tcgcgctctc gcggcggatc
    26581 gcggcccggg cgggggagct tccggtggac gtcctcttcc tcgcgccggc ggccgaggag
    26641 gcgggcctcc tcggctcgga cctcttcgcc cggacgccgc cggtgccgct cgcgcggatc
    26701 gcggcgaacc tcaacctcga gatgtcggcc gtctggggcc cggcgcgcga cgtcgtcgcc
    26761 atcggcggct ccgagagcgc cctcggagcg gtcgtcgagg cggtggcgaa gaaggaaggc
    26821 cttcgcgtcg cgccggagcc cgcgcccgac cagggcttct tctaccgctc cgaccagttc
    26881 tccttcgcga aggcgggggt gcccgggatc tggatcgacc tcggcgacga cctcgtcggc
    26941 acgccgcccg gcacgggcct cgcgcggcgc gaggagtacc gggcgaagcg ctatcaccgg
    27001 ccgaacgacg agctcgaccc ggcgtgggag ctgaccggca ccgcgcagct ggcgcggttc
    27061 gtcgagctcg ccatcgagga gatcggcgcc cgcggcggga aggtcccctg gaggtcaggc
    27121 tctccgtacg cccgctgagc gagggggtag catgagggcg tgagcgacac gcccgaacga
    27181 cccgtcgacg gcgaggaggc cgagcagtcc ggcttcgtct ccctcggcct cgactcccgc
    27241 ctcctcgccg cgctcgcggc gctgggctac gaagagccga cgcccgtgca gcggcaggcg
    27301 atcccgatcc tcctcgaagg gcgcgacctc ctcgcgcagg ccgcgaccgg cacggggaag
    27361 acggccgcgt tcgcgctccc gatgctcgac aggctcgtgc gggagggggg agagcgccgg
    27421 ggcgtctttg gcctcgtcct cgtcccgacg cgcgagctcg cgatgcaggt ggccgaggcg
    27481 gtccaccgct acgggaagca ggccggggtg agggtcctcg ccgtctacgg cggctcttcg
    27541 atgggcctgc agctccgggc gctcgagcgc ggagtggacg tcgtcgtggc gacgccgggc
    27601 cgagcgctcg accacgtccg gcgcgggact ctcgcgctcg ccgacgtgaa gctcgtcgtc
    27661 ctcgacgagg cggacgagat gctcgacatg ggcttcgcgg aggacctcga ggcgatcctc
    27721 gacgcgacgc ccgacgggcg tccgatggct ctcttctcgg ccacgttccc gccgcggatc
    27781 gccgtgctcg ccgcgcgccg gctctcctcg ccgacggagc tgaagctcgg agcatcccgc
    27841 gcgatgggag cggccccgga agggcagggc cgggtgaagc agacggccta cgtcgtgccg
    27901 cgggcccaca agctcgccgc gctctcgagg gtcctcgacc tcgaggaccc gacttccgcg
    27961 atcgtcttct gccgcacccg gaccgaggtg gacgagatct cggagaagct gaacgcgcgg
    28021 ggccgccgcg cggaagcgct ccacggcggc ttctcccagg agcagcgcga ccgcgtgatg
    28081 aggcgtttcc gctcgggcgc ggcggacctc ctcgtggcga ccgacgtggc ggcccgcggg
    28141 ctcgacgtgg agcaggtctc ccacgtcttc aacttcgacg tcccgagcga ggcggaggcc
    28201 tacgtccacc ggatcggccg gacggggcgt gcgggacggg ccgggagcgc gatcacgttc
    28261 tacgagccgc gcgagcagtg gctcctcgcg aacgtcgagc ggctcacgaa gaagaagatc
    28321 gagagggcga cggtcccgac cgtcgccgac ctgaaggcgc gccgcctcga gctgacgcgc
    28381 gcctcgctcc gggagacgat cctggggggc gggctcgaca ccttccgcgt cgcggtggaa
    28441 tcgctcgccg acgagttcga cctcctcgac gtcgcggcgg cgggcgtcaa gctcgcgcac
    28501 gaggccgcgg ggtcgccggg ggaggacgag gagatcccgg cgatccccgt tccgccggaa
    28561 cgcccccgct tcgcgccgcg cggcgacggc cccgcgccgg gtcccttccc ggcccggcgc
    28621 ggcccgcggc tcgcgggagc ggtgcgcctc ttcgtcgggg ccggccgcga cgcggggatc
    28681 cgtcctgccg acctcttcgg cgccatcacg aacgaggcga agctcccgtc gcgggccatc
    28741 ggggcgatcg agatcgccga ccgcttctcg ctcgtggagg tcccgggcga atcggccgac
    28801 gccgtgatcg aggcgctccg ccggacgacg ctgcggggac ggaaagtcct cgtccggcgg
    28861 gaacggcccg caccgccgcg gcgataagtc ccggcgcggg cgcgctcgtc cgccgacgcg
    28921 cggtgggcga gagccgcccg gcggcgcgcg gcggcgaccg gctcacgccc gtccggtggt
    28981 ccgtttgctc gcaccggccg gatcggctac cttgcgtaaa acagtcacgg tcggcgggtc
    29041 gcggtgtgcc cgcaaatcga aggaggacct gaacagatgc gccctctgct cctggcagga
    29101 gtgctcacag cgctcgcgac agccacgtcc gctccggcgg cgacgtttct cgtgaccacg
    29161 acggccgaca gcggccccgg gtcgctccgg caggcggtgg ccgacgcgaa cgcgagcacc
    29221 ggccccgaca cgatcgcgtt ctcggggctc gcctggccgg cgacgatctc tctcgtcgcg
    29281 ccccggatgg acgtcgtcgg cgacctcacg atcgacggac cgggtccgaa gctcctcacg
    29341 atcgagggag acggcacgat cacgggcctc ttccggctga accgctcggc cgacgttcag
    29401 aaccggctcg tcctccgggg ggcgacggtt cgctcgttcc ggttcctcta cggcggcgcg
    29461 atcgagggct acggagcaac gggccccgag ccgctcgagt ccgtcatcga ggtcgacggc
    29521 tgtgtcttcg aggagaacgg atgggccagc ggggacccga acgcgcaggg aggcgcgatc
    29581 tcggcctccg gcgcgctgac gatccggagg tcggagttcg tcgggaacac gaccggcggc
    29641 aacggcggcg gcgccgtcca gtcgtcggga tcgctcctcg tcgaggacag cctgttctgg
    29701 gggaactcgg cagagagcga cggcgggggc gcgatccgga tcctcggctc gggcgccgtg
    29761 acccgccgga tcgtcaactc caccttctcc gacaaccacg tcaccgtcga cagcgcgaac
    29821 aacgacggcg gtggcgcgat cgccttcgtg ggcctcggcg ccggaaccgg cctgcaggtc
    29881 tacagctgca ccttcgcggg gaacacgacg gtccagggcg gggggggcgc gatcctgttc
    29941 acgagccagg ccgccccggc cttctccagc ctcacgatca gcagcagcgt cttcgcgggg
    30001 aacacgtcgg gcggctcgcc gaacgacctt tccccggccg cggacgcgag cgtcacggcc
    30061 gactacagcc tcttccaggc gcacgcgagc gccgcggcgg ccggtatcga cagcaccgtc
    30121 tcgagcctct tcggcgtcga tccgcttctc gcttcgatcg tcgacaacgg cggtccgacg
    30181 cggacacggc ggcccgggat cggctcaccg gccatcgacg ccgggacgaa cgccctcggc
    30241 ctctcctggg atcagcgcgg ggcgggattc ccgcgcgtcg tcggcggcgc ggccgacgtc
    30301 ggcgcccacg agacgggcac gacggacctg ttcgccctga ccgtcacccg gaccggtccg
    30361 ggcgccgggg ccgtcgcgtc ggccgacttc gggatcgcct gcggaagcga gtgcaccgac
    30421 gtctacgggg ccgggaccgt cgtctccctc acggcgctgc ctgaggacgg ttcggttttc
    30481 gcgggctgga gcgggggagg ctgctcgggg accggcgcgt gcgccgtccc gatgaacgcg
    30541 acgcagtcgg tgtcggcgcg gttcgagatc gggccggcgc cgggaggcga gacgctgacc
    30601 atcacgatcg tcgggccggg gagcgtgacc tccgttcccg cagggatcga ctgcgcaccg
    30661 acgtgcagct tctcgttcgc ggccggagct caggtgacgc tgagccctgc gcccgccgcg
    30721 gactgggcgg gcacgtccgg tgatccgtgc gtgcccgggg cggcgacttg cacggtgacg
    30781 atggccgacg cgcggaacgt gacgttctac ttcctcgact cggcggcctc gccggtcgcc
    30841 ctgaccgtga ctcgttcggg aagcgggacg gggtcggtct cgagcgacct gccggggatc
    30901 gcctgcggcg ccgactgcgc ggagagctac acgaagaact ctctcgtcac gctcaccgcg
    30961 gctccgcaag ccggctcgat gttcgccgga tggagcggcg ggccgtgcag cggcacggca
    31021 ccgacgtgcg tcgttccgat gacggaggcg cggactgcga acgccgcgtt cgctctcctg
    31081 gagccggcgt ccgtgcccgg cctcggggcg ttcgggggcg tcctcctcgc cagcctcctc
    31141 gccgcggccg gcgctctcgc gatccgcctt cgttgaagcg attccggccg ggccgggccg
    31201 ccgtggggcg gcccggcccc gcgcctaccc ccctgagcgg tggctcgatt ctgctatctt
    31261 cgcggcacga tccaggacga aacgggcggg aatcgccgac aatacacgat ttcacgcctg
    31321 accccgtcga cgggagggac gatggcgacg aagaaggaga agccgttggc ggagcggttc
    31381 gcggaggcga aggggcgcgt cgagcagctg acgaagcggc cgacgaacga ccagctcctc
    31441 gagctctacg cgtgctacaa gcaggcgacg gagggagacg tcgcggggag ccgccccggc
    31501 ctcctcgacc tgaaggggcg cgcgaagttc gacgcctggg cgaaggtcaa ggggaccggg
    31561 aaggacgagg cgatgaagaa gtacgtcgcg ctcgtcgacg cgctccgcgc cgagctcggt
    31621 tgacgtgatc ttcctcgacg agctgcgcgc cgagcacgtc ctcatcgacc gcgtcgccgc
    31681 ctcgctccgg agctacgccg cggcccgggc ccggggagag ggcgacgcct cggacggggc
    31741 cgcgttccgg cggttcttcc ggctctgggc cggggcgtgg catcacgcac gcgaggagga
    31801 gacgctcttc ccggcgcttg cggagagagc cgagctgtcc ctctctcgcg gaccgctggc
    31861 ggcgttcgtc gagcagcacc acgacatggc ggcgcagatc ggcgcgttcg agccgctcct
    31921 cgaccgcgcg gtgctgacgg aggaggaggg gcggcggctc gtcgcactcg tcggcgccta
    31981 cacgaccgac ctgggccgcc acatcgacgc ggagaactcg gtcctgatcc cggagagcgg
    32041 ggtccggctc gcgcaggtca acgtcctcga gctgcacgga cggacgccgt cggccgagga
    32101 gctcgcggcg cgggcggagg gagagcgcct cgccgccgcc tggccggccg cgcccgaccc
    32161 gggcgtgcgc ggcgagggat gcgtcgtctg tccgtcgttc ggcacgacat gtcgcggcgt
    32221 ggaggccgag tggtggaacg agagcgagtg ggaggagttc tcggaccacc tcgggtgagg
    32281 ggccggggaa gggtgactcc gacgcctcgg tgacggggcg cacggaaccc gcgcccccga
    32341 ttccgcgtat tcgtctccga tcgaacgagc atcggcccga aaccgcgtcc cgcgggacgc
    32401 gggcgggcgt gtggctcgtc ggcggatcga cacgagctcg ggacgctctg cggagagcgt
    32461 cgccgaaagg agcggacatg gcctcggaaa ccaggagcta cctcgccgtc tcgaaggagg
    32521 ccgtcgcctg ggtgaaggcg atggtgccct tcggtgcctc gaaccggccc tcggactacg
    32581 tcgagagctg gaagaagaac cggaagaaga tcgagggccc ggaccccggg gtcctcgaca
    32641 aggtgctcgg agggatgacg gcgttcctgc ccctcctgac cggggcgaat cgtgcgatgc
    32701 agatcggcaa cgaggtctcc gggaacgcgc cgtccgacta tgtctcgcgc ttccgccaga
    32761 tctccgagat cacgctaagg acgaaggcgg gcaactgcaa cgaacagagc atcacggcct
    32821 tcgtctggct cctcgaccgt ggcgtgaggc cactcgcgtg gatgcacctg acgaacggca
    32881 agcacgcgtt cgtcgtcgtc gggcggaagc ccgggagcgg cgccgacccg acgaagtggg
    32941 gcgacgggtg cgtcgtctgc gatccgtggg cgggcgaggc ctattccctg ccggccgtcc
    33001 aggcgggcgc gatcctccag acgaagtggg gctgcggcac ggcgaacgcc gagttcggcg
    33061 tcgcgtagcg gctacgcggg ctggccccgc tcgacggcga gccggacgaa gtcgtcgagg
    33121 agctcggcga ggcgcttctc gccgcggctc ttcgcggacg gaatcggctc gccggcgcgc
    33181 ggcgactcca cgagctcgaa gtagtacttg atcttcggct ccgtccccga ggggcggagc
    33241 gtgacgcgcg agcccccggc gaggaggtag gcgacgacgt tcgacttcgg gagcccgggg
    33301 acgccggcga ggtagtcgcg cgtctcggcg acggcgagac cgccgatggc ggccggcggc
    33361 cgggcgcgga agccgtccat gatcgcggcg atcgtcgcgg ccccttcggc ccccgggatc
    33421 gtcacgctct tctgcgcgga gaggaagagc ccgtgctcgc gctggatctc ctccaggaag
    33481 tcccacaccg tgatcgaccg ggcccggcac cagccggcga ggtcggcgaa gacgagggcc
    33541 gcggagatgc cgtccttgtc gcggacgacg tcgccgaccg agtagccgag cgcctcctcg
    33601 tagccgaaga cggtcgtcct ctcctcggtc cgctccagct ccagggctcg gttctcgatc
    33661 cacttgaagc cggtgagcgt ctcggcgtac gcggcgccga ggtcgcgcgc gacctttccg
    33721 agctggctcg aggagacgat cgtcgtcacg acgaggggct tctccggctt cggacggggc
    33781 tccttgaggc agtaggcgcc gaggaggacg ccgatctcgt tcccggagaa gacgcgcatc
    33841 cgtccctgac ggtcgcgagc cccggcggcg agccggtcgg cgtcggggtc gttcgcgagg
    33901 aggagatcgg cgcgcgtctc ctcgcagagc gcgagggcga ggtcgagcgc ccccttctcc
    33961 tcggggttcg ggaacgcgac ggtggggaag gcgccgtcgg gctcgttctg ctccgcgacg
    34021 ggggcgacgc tcgcgaagcc ggccttcgcg agcgcggcga gcgtgaagcg ggcgccgacg
    34081 ccgtgcatcg ccgtgtagac gatccgcagg tcgcgcccct cgccgggatg gagggcgagg
    34141 cgggcgattt ccgcgtggta tcgctccgcc atctcctcgc cgagggggac gaagagtccc
    34201 cgcgcctcgg cctccgcgcg cgggacgaag gggagcgcgc ccgccggccc ggctttcgcg
    34261 atctctccgg cgatcccggc gtcgacgggc gggatgatct gcgctccgtt ctcccagtag
    34321 accttgtagc cgttgtactc gggcgggttg tggctggccg tgacgacgac cccggccgcg
    34381 gcgccgagct ccttcacggc gaaggcgccg agcggcgtcg gggcgcggtc gacgaagaag
    34441 aagacccgga cgccgtgacc ggcgaggacg ccggccgcct cgagggcgaa ctcctcgctc
    34501 ccgcggcggg cgtcgcgcgc gaggacgacc ccgcgccggg ccgcgtcggg gacggtcgcg
    34561 aggaggtgcc gggcgacgcc ggccgtggcg cgcctcacga ccgcgcggtt catccggttc
    34621 ggcccggcgc cgaggaggcc gcgcaggccg gccgtgccga actcgagctc ggaacggaag
    34681 cggtcttcga gggcggcctc gtcgccggcc tcgaggagat ggtcgacctc gaggacggtc
    34741 gcggggtcgg ggtcggcgtc gcgccaggaa cgggctcggg cgatcagctc ggcgttcatc
    34801 cgggagatgg tatcgccccg cggctcaggt gcgcggcggc gtgacgatgg cgaagccggc
    34861 ctcgttcgac ttctcgtccc agatgccgat ggcgacccgg gcgccggggc ccgtcgtcac
    34921 gacgtcgagc tcgtaggtgt agtgcccggc ctgggccttc tcgaggtcct tggccgggat
    34981 ctcgaacggc tggttcaggc gcgtcaccgt cgagaagtcc ccttcggggg cgaccgacgc
    35041 cacgaagacc gagaagacgc cctggacgcc cttcgcggtc ggcaggagcg cgagggactc
    35101 gatcgggatc ctcacctcga gccggaggac gtgcttgtcc ttgcccgggg tcgtgccgac
    35161 gaggctcgcc gagatcggga tgcgggagcg ctcgtcgggc tggaagaggt gggcgaggac
    35221 ccggtccgac atctgctcct cgacgctctt ctcgacgatg gcgcggcggg cgcggaccgt
    35281 caggtcgcgg cccttcacgc ggacggagat cgaggccgtg cgacccgtcc cgggcctcga
    35341 cggaaagccg agggagtacc acgactgcag gtcgcgcgag acccgctcca cgaggcggcc
    35401 gacgcatccg gcgccggcga gggcgacgcc gcccgtcgcc gaggcgacga cgtcgagcgc
    35461 ctcgacctcg ttctgaagga gggcgtcccg gcggacgccc agggcggggc cgctcgccgg
    35521 gggaagcgct cgccccggcg cggcgggggc gccccggccg ccgccgatgt cgctgaagtc
    35581 gaagttcggg tgctcccacg ccggagagga cgtcggggaa tcggccgcgg aggggagtcc
    35641 cttcccctcg aaggcgtcgg gaaagaggcc gtagagcgtg acgccgttcg cgttcgcggc
    35701 ctgggtgacc tcctccagga gctttcgggc gtcctgcgcc cgccgcttcg cgtcctcgag
    35761 cgcgccggcg tcggggcggg agccgatgag gaactcgagt ccggggaagc gggagaagcg
    35821 gttcgtgacg agcacgagcg ccttccggcc atcaacgccg ccgagagtcg ccgcgaggcc
    35881 cttcagtgcg gccgtcttcc ccttcatctc gaagtacgcc tgccgcgcga ggatctccat
    35941 ggtcgtctcg cttccgtctc cggcggcggt cccgacccgc gggtcggccg actgagactt
    36001 gaaccaggac gtctcccact cgaggagcgc cgactcgccc gcctccgggc ccggccgggc
    36061 gcagcggcgc tcgatccgct ccagggtcgg ctccagggcg tccaggtcgt cggtgaacgg
    36121 caggaccgtc cggaccgagc gctcccaggt gacgatcatg acgtcgtccc cgtcgtccag
    36181 ggtgtcggcg acgagggacc ggagggcgtc gaacagcgcc ttccggactc ccggctccgg
    36241 gaggtagagc cggtcgaaga agagaacgac gcggcggcgg gggcgcgccc cgggcgttgc
    36301 ggccggcggg gcggcggccg cggtcgtgcc cgccggtgcg gcggtgatcg cgggcaccac
    36361 ggcggctgcg ccctccaggc ggatctcgct gaagttcgtc agctcgaccg ggcgaccgtc
    36421 gtggaggacc tcgaaatcgg cggccacgag gccgcggacc gggtctccct tcgcgtcggt
    36481 cacgaccaca tcgagattcg tgaggctcag ctcgacggag gcggtcgtgc ggggaggggc
    36541 ctgtggggca gcggcctgcg ccgccgccgg cgccccgtgc gccgcgaacg cggagaggag
    36601 aagacacgga aggagagctc gccggccggg cctgatgggc atggaacgcc tcgcgcgcac
    36661 gagtctagtc tcgctcacgt gcgcgaggga gtgacgagcg cgaagccggc ctcgttcgtc
    36721 ttctcgtccc agatgccgat cgcgattctg gcctcggggc tcttcgtcac gacgtcgagc
    36781 tcgtaggtgt agtgcccggc cttcgccttt tcgagttccg tctccgggat ctcgaagggc
    36841 tggttcaggc ggttcacggt ggagaaatcc ccctcgggcg ccactgcggc gacgaagacg
    36901 gagaacgcgc ccccgacgcc tttcgcagtc ggcagaagcg cgagcgatcc gatcgggatt
    36961 tgcacctcga cccggaggac gttctttccc ttctcgggcc tcgagccgac gaggctcgcc
    37021 gagatcggga tgcgggagcg ctcgtcgggc tggaagaggt gggcgaggac ccggtcggac
    37081 atctgctcct cgacgctctt ctcgacgatc gcgcggcggg tccggacggt caggtcgcgc
    37141 cccttcacgc ggacggagac cgacgcggcc cgcccggtgc cgggcctcgt ggggtagccg
    37201 agggagtacc acgactgcag gtcgcgcgag acacgctcca cgagccggcc gacgttgccc
    37261 ccgccggcga ggacgacgcc cccggtcgcc gaagcgacga tgtccagcgc ctcgatctcg
    37321 ttctggagca gggtgtcctt gacgattccc tgcggcggcc cgccgctccg gccggccgag
    37381 actacgtcca gtccctcgaa cgcatcgggg aagagcccgt agagggtgac gccgttggcg
    37441 ttcgccgcgt tcgaaacggc gtccaggagc ttccgcgcgt cctgcagctt gcgcttgctc
    37501 gcgaggatct gctcgatgtc cgtggcgctc cggatcagga actcgagccc cgggtaccgg
    37561 gagtaccggt ggctgacgag gacgaggacc ttccgcccgt ccgcgcctcc gagggtcgcg
    37621 gcgaggccct ggagtgcagc ggtcttcgcc tgcatctcga aatacgactg ccgggcggtc
    37681 agctctgccg tcagctcgct gtcgccgggg atgccctcga atcgcgggtc ttccttcagg
    37741 gattcgaacc aggcctgctc ctggtcgagg ttgatgtagt cggtctcctc ccggcccggt
    37801 cgcgcgcagc gctcctcgat ccgcccgagg gcccggcgca acgcgccggg atcgtccgtg
    37861 aacggcagga ccgtgcggat cgacctctcc caggtgacga tcatgacgtc gtccccgtcg
    37921 tcgagcgtct ccgcgacgag ggaacggacg gagtcgaaga ggttctttcg tatgccgggc
    37981 tcggggagat agaggcggtc gaagaagagg acgacccgcc ggcggggggg cttcgccggc
    38041 gcggcggacg gctcggtggc cgtcggggac gcggcctcga gagccgtccc cccttccagc
    38101 cggaactcgc tgaagttcgt cacctcgaca ggccggccgt cgtggaggac ctcgaagtcg
    38161 gcggccacga gcccgtggac cgggttcccc ttcgcgtcgg tcacgacgac gtcgaggttc
    38221 gtgaggctca gctcgacgga agcggtcgcc cggggaggcg tcggcggcgc agcctgcgcc
    38281 gcggggagcg cgacgttcag gactgcggcg gaaaagagga ggggcgtgag gctcgcgcga
    38341 aacccgttca tcagcacgta cggcacgcag gatgcgtgcc gtacgtagag gtacccttcg
    38401 gaggacggcg ggaaggaagc atgatctcgt tcgtttcgct ttttgtcggg ttcgtcgtcg
    38461 ggatcgtcaa cgtccagctc atggcgaccg ccgaggtcga ccgggtcgag ctcttcctcg
    38521 acggccaggt cgtcgcggag ctgcgcgagc cgttctcgag gcccgtggac ctgggctgcg
    38581 agccggcgcc gcatgagctc gtcgccgtgg cctacgacga gcgcggacgg gtgctctcct
    38641 ccgcccggca gtgggtgaac cggccgcggg cgaccgccga cctgagcctt ctcgtcgacg
    38701 gcggcgggga ctcctcgccg cggacggcga ggctcgtctg gcgcgccctc gcgggggagg
    38761 tcccccgttc cgttacggtc acgttcgacg gcgcggtgct gccggcaccg gatccgcagc
    38821 ggatcgagct tcccgagcac gacccggcgc acgtccacct ccttcgcgcg gacatcgact
    38881 tcgggcgggg cgtcgtcgct tcggcggaga tgatcgtcgg cggaccctca cgggtcgaag
    38941 cgcgctcgga gctctccgcg atcccgctgt cgatcgaaga gggcgccgcc ctgcccgcgc
    39001 cggagggcct cgccgggtgg ctcgaagccg gaggacttcc gctcgagatc gcggcggtcg
    39061 aggacggccc cgtcgacgtc gtcgtcgtgg gggagcggag cgtgccggag gccttgaagc
    39121 gcttcggacg ccgcagccgg cgctcgcggc gaagtcgctc cgaactcccg gcgacggcac
    39181 cggcggtgcc gtcggaagct ccggcgcctg tcgccctgga cgtccggctc cggttcctct
    39241 ggcccgtcgc caccatctgg acgcagtccg ccatggtggc gaacaacttc ctcgcgagct
    39301 cgtcctcgga cgaggcgcgg ctcgacctcg cctggctgac cgcgcggcac ggcgactggc
    39361 cgaactggag cgggacgtcg gagcggctgg cggacgccgt cgcggtggcg gccctgacgg
    39421 ccgcggaggg gaaccgccgc cgcgcggtcg tcctcgtcct cggaccctcc gcgaaggacg
    39481 acagcctcgt gacggcggag gagaccgcac gcttcctcgg gcggatcggc gttccgctgc
    39541 acgtctggtc gatcagcgag accccgtccc ccgaggcgtc gcggtggccc gcaccgcgga
    39601 cgatcgcgag cgcggccgat ctcgacgacg ccgtccggga cctcaccgca gcggcggctc
    39661 gccagcggat cgtctgggtg gagggatcgc acctgccgca ggacgtggag ctcgggccgc
    39721 tcgcgaaggg gatccggctc gcgcgctgag gcgtctgggc cggcccgggc tcggcgcgcg
    39781 tgggaggccg gcctcctttc cgtgcgctgt cgaagacgtg gcacgtgctt cgcgagcgtc
    39841 actcgagtgt cctttccggg atacagggag gacggatggt ctctttcgcg tcgctcttcg
    39901 tcggattcgt gatcggagtc gtgaacgtgg agctgatggc gagcggaggc gtcgacaggg
    39961 tctcgctcct tctcgacggc cggccggtcg ccgagctgcg cgagccgttc cgggcggcgg
    40021 tggacctcgg gtgtgatccg gccccgcatg agctcgtggc ggtggcctgg gacgctgcgg
    40081 ggaaggagct cggccgggct cggcagctga tcaacctgcc gcggccgacc gcggaagcga
    40141 gcctcgtcct cgaggcggga cggggcggca cgggacgggt ggcgcgcctg acgtggcggg
    40201 cgctcgtcgg agaggtgccg aaggcgctgt cggtgacgtt cgacggggag ccgctcccga
    40261 tcgccgatcc gcgccggatc gagctgcctc ctcacgagcc ggcccaggtc cacttcctcc
    40321 gcgcggagct cgagttcggg gaaggcgtgt ccgcctcggc ggagctgatc ttcggcggct
    40381 cgaagcggtc cgaggcgctg gccgatctga cggcgattcc ggtggagctg gagggagagg
    40441 cctcgcctcc gtccgccgcc gagatgacag gctggctcga ggcgcgtgga gtcccgctcg
    40501 aggtcgaggg cgtcgaggcc gccggcgtcg acgtcgtctt cgtcgtcgag gggagcgcga
    40561 tccggcagct gaggcgggct gcgagccgag gcgccgacga gtaccgccgc agctcggact
    40621 cgttcctgga tcctcggaag agcccgagcg gaacgcccac cttccgacgc tggggaggca
    40681 cgggcggtcg tcgtctctcc gtccccgtcg atctccggtt tcggatacag cgtcccgtgg
    40741 cccggatgac ggcccagaag gagatggtcg cgaacctctt tccgtcgacc cgctggttcg
    40801 ccttcagggc gctgacgccc gggagactcg tgtacgccct cctctccttg tccgaagagc
    40861 caccgatcgg tcagcggctg gcggacgccg tggtcgtcgc ggggctcgag gcggcgaagg
    40921 ggaacggacg acgagcggtc gtcctcattc tggggccggg cgcgaaggac gagggcctcc
    40981 tcggagccga ggacgcgagg tcgttcctgg ctcggctcgg agttccgctg caggtctggt
    41041 cggccggacg gaagcactcc cgggaggcgg cccggtggaa ggcgcagcgc ctgatgtccg
    41101 actcctggga agtggaaggg gccgtccacg agctcctcga ggcagcgctc cgccagcgga
    41161 tcgtctgggt ggaaggatcg cacctgccgc aggacgtgga gctcgggcct ctcgcaaaga
    41221 ggatccggct cgcgcgctga tcccggtccg tcgcccgggg aggagggaag cccatggtct
    41281 ctttcgccac gctcgtcttc gggctcgtct tcggcatcgt cgacatcgag gtccgggcgc
    41341 cggaggcgac ggagagggtc gagattctcc tgaacggaga gtcggtcgcg cagcttcggc
    41401 gcccgttctc cggcgcggtc gacctcggag ccgtgccgat tccgggggag ctcgtggcga
    41461 tcgccaccga cgccgagggc cgggagctgg gacgctgttc ccagcggatc aacgcgcccc
    41521 gggcgaatgc ccgcgcgcgg ctcgtgcgcg agccggacga cgagaaggga cgggcggtcg
    41581 ctcgcctgac ctggagctgc ccgacggacg ctcccccggg ctcggtggat gtcacgctcg
    41641 acggccagac gattccggcc gcggaccccg atcggatcgt cgtgcccgct acggacgcga
    41701 gaggcccccg ccttctccgc gcggtcgtgg agtgcggccg cgaagtccgc gccgtcgcgg
    41761 agatcgggat cgggagagat ctgccggata tcccctcgga gcggacggct gtcgcggtgg
    41821 agctggacgc gggagcgacg cttcccgcgg tcgacggcct cgggggctgg ttcgcgacgg
    41881 agagcggacc gctccgggcg tcggccgtcg atgaggtcgg gctcgacgtc gtcttcgtct
    41941 gcgagccgag cgccttcagc gagctccgcc gcgtcttctc gaagagggca ggccccagca
    42001 gatccaccgc gagggaggac ctgcagttcc gtttccttct gccggcggga ggcggcgccc
    42061 ccggctccaa cgtgctcgac tggggacgga acggcttcgc ggcatcgaag tggttcccgc
    42121 ggaagtggaa ggccgtcggg ccgaccctcg ccacggggat cgaggtcgga gatttcttcg
    42181 ccccgggaca gcggatctcg gacgccgtcg cgctggcggg attcgccgcc gccgggaggg
    42241 agcggcgacg tgcggtcgtc ctcctgctgg gtcccgccgc gcaggatgtc agcgagctgt
    42301 cggcggcggc ggcggcgcag ttcatcgctg ccctgaacgt gcctctcttc gcgtggtcgg
    42361 tcgggccatc gtcgtctccg atcgaggaga tgtggagcgt gcggagacgg gtttcgacgg
    42421 cagcggagtt cgaggccgcg ctcgcagacc tccgcgagga cctgctgcgc cagcggatcg
    42481 tctgggtaga gggatcgcac ctgccgcagg acgtggagct cgggccgctc gcgaagggga
    42541 tcc
//
LOCUS       DAOONL010000024        41766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_685_length_41766_cov_17.720769, whole genome shotgun sequence.
ACCESSION   DAOONL010000024 DAOONL010000000
VERSION     DAOONL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 41766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41766
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_41766_cov_17.720769"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /gene="nadE"
                     /locus_tag="PLP50_05820"
     CDS             <1..419
                     /gene="nadE"
                     /locus_tag="PLP50_05820"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPA51104.1"
                     /translation="YCTLYGDMAGGLAPISDLPKMRVYELSRHLNRRAGRELIPASTL
                     EKAPSAELRPNQTDQDSLPPYALLDRILEGLVAGNLPPAKVARETGAPVALVAGLARQ
                     IDRAEYKRQQAAPGLKVSTKAFGSGRRIPIAQVPGT"
     gene            416..1117
                     /locus_tag="PLP50_05825"
     CDS             416..1117
                     /locus_tag="PLP50_05825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51105.1"
                     /translation="MKYAGDLPRSRYPWKEIPGSSHAVLLGRVAALGDSLSVLDVGFG
                     AGHLARRIRPRCRYLAGIELDPEAAREGSAFFDDPVLDDVVRGLAGPWREAFDVVVAA
                     DILEHLPEPGQALDLLRPLLREEGLLLVSLPNVANVTVRLALLLGRFPRTQRGILDRT
                     HLGFFTRRTGRELLEAHGYRVVRETPTAMPVELAFPVLGRPPLSAPVRAAATLLARAW
                     PGMFGYQFVYEARPA"
     gene            1114..1866
                     /locus_tag="PLP50_05830"
     CDS             1114..1866
                     /locus_tag="PLP50_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA51106.1"
                     /translation="MTRPPVLTLVVPAYNEEKRLPVSLARISEWVLSRTPQLSVEVLV
                     VDDGSSDRTAAVAEKTAAGLGLDFRVLRLLRNRGKGAAVREGVLAAAGEQVLVTDADL
                     STPIEEVDKLLAAGAPVAIGSRAVDETLVKQRQSLFRVASGKLFNLLVRLLAVSGIRD
                     TQCGFKLFRADAAREIFSRATVDRFAFDVEALLLARRLGYPIAEVPVLWFNSPDTRVG
                     LGGGLEAFAALFRIRRRVARTLRARPPAPRAS"
     gene            1931..2596
                     /gene="elbB"
                     /locus_tag="PLP50_05835"
     CDS             1931..2596
                     /gene="elbB"
                     /locus_tag="PLP50_05835"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPA51107.1"
                     /translation="MKPKVGVVLSGCGVYDGAEVHESVLTLLALDRAGAEAVCLAPDV
                     AQRHVVNHLTGQPAEGESRNVLVEAARIARGQVKSLAGFDPAPLDALVLPGGFGAAKN
                     LCDFAFRGADCEVHPDVASVVRAVHAAGKPVGAVCIAPVILAKLLGAEKPKLTIGTDA
                     GTAAAIGKMGGEHVSCGGGGAVVDAERRLVTTPAYMLDSPISQVSAGIEKLVGELLSM
                     VRG"
     gene            complement(2604..3581)
                     /locus_tag="PLP50_05840"
     CDS             complement(2604..3581)
                     /locus_tag="PLP50_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51108.1"
                     /translation="MSVLGWLPRKLGRIVADQVHDRLAPVLRDADGRFAALALRSGPQ
                     PAPAMGGTTISFDYPTSAANRPRWTAEAGSHPGVRRLIERDEPVWTETVRRISERGEE
                     LVRRHGGSGIPETADDADPRPRWRNAMMAGLDGVAFAAFLGPETRQVLEIGSGNSTKF
                     LRHLIGALELKARIVSVDPFPRAECDALCDTVIREPLETVDLALFDGLRPGDVVFFDG
                     SHRCFMNSDVTAFFLDVLARVPTGVAIGIHDIFLPDDYPEEWAERYYSEQYVLAAWLL
                     GAEGRYRVTLPAYWVSRNRERLGLDAIADAYGCPRDRFDGCAFWIEKTG"
     gene            complement(3682..7515)
                     /locus_tag="PLP50_05845"
     CDS             complement(3682..7515)
                     /locus_tag="PLP50_05845"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPA51109.1"
                     /translation="MPRTTERRSSGLSTVPRFTALRATLAFAIAAVLAGPAAGQKITE
                     VVFDHVGSPDTHEYVELWADPSADLSSFRVVVLDGSSNPGQILNVVTPGTANGAGFWS
                     SGSLTPLLESPVFTVLLVSGFTGLVGDDLDSGNDGVLDANPWSSIQDGVAFSDGTAGA
                     RTYTAPVLGPGFDGNATAPGGASRFPYYVDTNSVTDWKRNDFDGAGLPGFSGTLVSGE
                     AWNTPASVTRVHLEDYYAGVDASSQAALRSTIHAAIENHIWFPYTADTTDTWDILNLA
                     EQDPVDAGMILDKYKNARYTKIAGGTGIYNREHSWPNSYGFPSNGDVPYTDCHHLFAT
                     NTEYNGNRGNDPFGTCNSGCAEDETDLNYGFGGGSGTYPGNSNWSSPSSRIYEVWNHR
                     RGDLARAQLYMDVRYEGGTHAINGKSEPQLILTDNLSQITGTSGGTAYMGRLTVLLQW
                     HLADPVDDDERARNDVIASFQGNRNPFVDHPEWVECVFQGICGGNPGIVFSGIQSATD
                     PNLCATGGVGVTWNAPSSWNDDCTAGCDRGFHVLRDGSAITSGGCAGPLTASATSCTD
                     TSGTTGVSYAYAVEAFNHEAETSTGNTSISAADRADDGLAPVLTAGPSASSAVSSSFT
                     VAWTTDEPSDSRLEYGLTAGYGSSAYDATPVTAHSLTVTGLSPSTEYHFRAGSTDPCG
                     NGPAWSSDGTVTTAAVATTLDIGGWQLVQANSTQTYTLPAGTTVPAGGYVVIGRNVEK
                     TAFETAWGVTLGPDVVYLNSGETIPMINGGETYTLKNASGVIVDGPTIALPATFQSVR
                     RNDPCQPAGSAGSWTAGALSTATPGTGAGAGCGTGLVINEFSDASAFANEFVELHWDS
                     SAVEPAPTVATGAASGVTTTGATLNGIVNPNGLATTASFEWGLTTSYGQTTAGQSMGS
                     GSAALPLSASLTGLTACTTYHYRATATSSAGTTNGSDATFETSCPAPVVTTGAASDVT
                     TTGATLNGTVNPNGLATTASFEWGLTTSYGQTTAGQSMGTGSAALPLSASLTGLTACT
                     TYHYRATATSSAGTTNGSDATFETSCPAPVVSTGAASGVTTTGATLNGTVNPNGVATT
                     VSFEWGTTTSYGQTTPGQAIGAGTAAQAVSAALSDLAPCTTYHYRTTATSAAGTTNGE
                     DATFQTTCTGGRFYPLAPCRVLDTREDGPAMTDGVPREVAFHGACGVPAAARALATNV
                     TVTQPTLPGHVSVYPADAASTGTSVVHFVAGKTRASNVIIKLSDDGTGRARLEATVPV
                     GGTVHVIVDVSGYFD"
     gene            7903..11211
                     /locus_tag="PLP50_05850"
     CDS             7903..11211
                     /locus_tag="PLP50_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPA51110.1"
                     /translation="MLAAIAGFVSSEARATVQPTGSMAGPRSEHTATFLGTGKVLVAG
                     GADSTGRLATAELYDPATGSFAATASMPEERHGHTATMLPTGKILVAGGNGDAGALAT
                     CRLYDPRTGTWTSTGSMATARREHTATLLANGKVLVAGGQNGTAPGYTALASAELYDP
                     ALGTWTATGALGSARAGHAAALLADGKVLVAGGQTRWIPLPAVYTATAFLYDPTAGTW
                     SATGSMTAARRSFTLTRIPGGLVVAAGGRNASYLSSAERYNVSAGTWGSAGALGAARS
                     GHAAALLPDARVLLAGGSDGTGPAGAMLEYSPGTNTWAEVETGGARVDHTATLLPGGR
                     VLVVGGEQSGWLSDAEIVDRMNPAWTTAAAWGQADGTATLLPSGKVLLLGKAVFGDLS
                     RGVLFDPETETAVSTTGAMVARRSKHTATLLADGDVLIVGGVDGSGALVAQDEIYDPD
                     SDTFTALAVPLPTRRAEHAAALLPDGRVLVAGGQASSFLAPYIYSGGVWTVVNEEHGY
                     DGWGVRLMVLANGHVLVRSGNLLLQFDPFPGALNTRWRTLDISSGNAFGEPVLLPSGD
                     VLFVQGNPVPRYTLWDVESSSFREPFPAIPPAIPLRFGFRALALPDGRVLVTGGYGGD
                     SGVDPLAHAAVLDPATGVTTPLPSMAAERSNHTMTLLPNGRVLVAGGVIGSSTTIEAT
                     VETLRLWPGLAESSRPVITSWPASLSLPGSFVLNGTGFLGIGEASGGNGSASSSVGFP
                     SVLLRRIEGGFSRWIPSFEGAAFGATAFTSSEVSGIPGGHYLATLFASGIPSKPKVVV
                     VSPCEIYVGVMGSTSDASVCVGDDLRLEGRLLSNVTNVVWTWTLPDGSTSTDRLLTIP
                     DAQVEDGGFYTVVASRPGCVGPVDTVEVRVAPPGQMPIITAPFTLLHAQEGEASVEWQ
                     LGSSWTWTLSGGSILRGQGTNRIRFRAATDGASMTITVVESVGESCDQPPSMAVVELM
                     APPVLYSPVTPCRLFDTRNDTGPAAAAPALGPGETRVFSVGTRCGLTSSAVRALAVNQ
                     TVTQPAATGDLVLYRGDVTTIPVASSVSFTAGKTRANNGILELSKFGDGTFRVHNRST
                     GTVHFILDVSGVFK"
     gene            complement(11506..12756)
                     /gene="glyA"
                     /locus_tag="PLP50_05855"
     CDS             complement(11506..12756)
                     /gene="glyA"
                     /locus_tag="PLP50_05855"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPA51111.1"
                     /translation="MGRSLSTVDPEIAAAVEAEFARQRDGLELIASENYASRAVLEAM
                     GSVLTNKYAEGYPGRRYYGGCENVDVAESLAIARAKKLFSPANPDAIHANVQPHSGSQ
                     ANTAVYLACLKPGDTLLGMSLAHGGHLTHGHPLSISGKQYRVVSYGVTRETETIDYDE
                     LARIAEAEKPKLIIAGASAYPRVLDFPRFRQVADSVGALLMVDMAHIAGLVAAGLHPS
                     PVGHAHFVTSTTHKTLRGPRGGLVLCTPEWAKEIDKAVFPGLQGGPLEHVIAAKAVSF
                     LEALAPEFKEYQGRVLTNAKAFAEAVAGQGFRIVSGGTDNHLFLVDLGPKQLTGKVAE
                     KALDHAGITVNKNAIPFDPNPPLVTSGLRLGAPAVTTRGMGVAEMKTIAGWIGEVLAA
                     PEDEAVKVAVKAKVKELTSAFPIY"
     gene            complement(12786..13244)
                     /gene="rpiB"
                     /locus_tag="PLP50_05860"
     CDS             complement(12786..13244)
                     /gene="rpiB"
                     /locus_tag="PLP50_05860"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPA51112.1"
                     /translation="MRIAFGADHAGFELKTLLARRTAELGHEVIDLGTSSTASVDYPD
                     FGYAVGEAVASGRADRGVVVCGTGIGISIAANRVRGARAGVATDLFSAQLMREHNDAN
                     VLGLGARVTAVPLATAILEAFLSTPFAGGRHQRRVDKLDAPPPSAQGASR"
     gene            complement(13254..14618)
                     /gene="dgt"
                     /locus_tag="PLP50_05865"
     CDS             complement(13254..14618)
                     /gene="dgt"
                     /locus_tag="PLP50_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPA51113.1"
                     /translation="MNAGRPPRAVAFTKRELEAFEDERLAIYASRAAASTRRREIPAE
                     ADGHDYRTEFQRDRDRIVHSKAFRRLKHKTQVFIPFEGDHYRTRLTHTLEVTQVARTV
                     ARTLGLNEDLAESCALAHDLGHTPFGHSGEKALNRILTGGEPSVSLSPKAVAAVGAFK
                     HNYQSVRVVDLLEKRYEEPGLNLTDETREGILKHTTWKRDFPFPVLDPDGLHLGSGCH
                     LEGQAVGWADEIAQQAHDLEDGLGLVGIGKAEEIPLAREIVAALGAAWRDASDPAVKR
                     ALLIRGLIHHLVTDLVVASRAAIEKWLTAQKVTTRAELAAHRRKLPGSLVAPSREGEK
                     RYLELKEFVYRHIIHSQAVSQHDARAQLVVNRLFDAYAKNPRLLPDAVLARVAELSGL
                     PFLREVPLKKVDGVIAGRWREEPAVLRCIADHIAGMTDSYALAEYDALFAPYPRRPAG
                     TPAG"
     gene            complement(14650..15855)
                     /locus_tag="PLP50_05870"
     CDS             complement(14650..15855)
                     /locus_tag="PLP50_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPA51114.1"
                     /translation="MIPGAGLRWLEALGPQTIRPGLARTRALLSALGRPQDSFRTLLV
                     AGTNGKGSTAATAAAVLEAAGLRVGLTTSPHLVRVNERVRLPGGRDVSDAVLDATLSL
                     VAAVSGEGPLRPTYFETLVVAACELFRRAGVEVAVVEVGIGGRLDATNVLEPEVSVVT
                     NVDADHLETLGPTLADVAREKAGVFRPGHLALTTERGGMLGVLHAEAARVGADLVEVM
                     PSAAWDDVSPLAGAHQRANVALGVAAARALVPADDATVRRGVAATRWPGRLQTVPRPG
                     ARPLLVDGAHNPAGAAAVAAYLDEVGLAGRVGLVFGALGDKAVEGMFAPLAARARQVV
                     LVAPDSPRAVRPDALAARLGRDDLPRAASLAEALALLAGGEEDAPILVAGSLVLAGEA
                     LALAGCRDA"
     gene            complement(15852..16655)
                     /gene="accD"
                     /locus_tag="PLP50_05875"
     CDS             complement(15852..16655)
                     /gene="accD"
                     /locus_tag="PLP50_05875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPA51115.1"
                     /translation="MRTATSLGEGHFLKCDGCRETLYAKEVERNQRVCPKCGFHFRLS
                     VDERLKLLFDDGAFQLLFGDVHPTDPLRFRDSKRYRDRLREYGGRTGRPDAIAAAEGT
                     IEGTRVLVAALDYAFMGGSMGSVVGEVLTRAAEKAAAEKVPFVVVTASGGARMQEGIL
                     SLMQMAKVSAALARLSEARIPYVTVLTDPTTGGVTASFAMLGDVTLAEPRALIGFAGP
                     RVIEQTIRQTLPEGFQRSEFLLDHGFVDVVVPRKELKATLANVLRLLAA"
     gene            complement(16716..17519)
                     /locus_tag="PLP50_05880"
     CDS             complement(16716..17519)
                     /locus_tag="PLP50_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51116.1"
                     /translation="MTPLELRAVFAGYARGKDVLRDVSLTFAEGRVAALLGENGSGKS
                     TLLKVLAGLLPPSRGTVLLRGRPISEVPRREAARQLGYLPQGFEPFFPATALDVVLLG
                     RTPFLGAFSTPSRRDVAIARAALEEVDAAGLEERDVRSLSGGERQRVYLARALAGEPR
                     VLLLDEPTASLDPRHRFLVLDVLRRLATRGVCCVFSTHEVDLAARTADDAVLLARGAV
                     LGAGPVRQSVTERSISELFGVAACVTPLADGTPLVTLGAPPAVRDGGRG"
     gene            complement(17516..18505)
                     /locus_tag="PLP50_05885"
     CDS             complement(17516..18505)
                     /locus_tag="PLP50_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPA51117.1"
                     /translation="MRSPGRFLALLALLALGAVVASLLVGSVPIAPTDALAALAGDGD
                     EATRTIVRDLRLPRALLGLLVGGALALSGAALQALLGNPLADPYLLGVSGGAACGTLA
                     AVVLGLGTAGLFGSFALPLASLAGALGAVLLVGLGASVGGRLDRGRLLLSGVVVNALA
                     SAVLLFLLSVGDASDTRGFVFWMLGSLAGATGDAVVSLAVYSAVGLVVLLPLARAFDA
                     LTLGEETAFTLGVSVERAKRIAYVAASLLAAAAVAHAGIIGFVGLLVPHLVRRLTYGH
                     RALLAGSFLAGGVLLVLSDLLARTLFSPNEISIGAVTALLGAPAFLVLLRRRA"
     gene            complement(18502..19356)
                     /locus_tag="PLP50_05890"
     CDS             complement(18502..19356)
                     /locus_tag="PLP50_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPA51118.1"
                     /translation="MRTRQILLAAAAVAWVAVATPLAAAPPRRIVVLAPSAAEIVFAL
                     GAADRVVGVSDFAAGLPGAEGKARLGGFVPDLERIAALAPDLAVVSRDGTDRRAAERL
                     AALGIRVVVTDATSLDGVYTDVRRVGAALGRDVEAERIVARMQRRVAAAEERVRARRA
                     TRRPTALAVIWPDPPIVAGRDTFVGDLLDRAGLENVVPKGAGEWPRVSHETLVGWNPS
                     LLVRPDTRENRAAFEAVLSPASRWQLLPAVRAGRVVVLDGDWLERPGPRLVDALETLV
                     AKLDAGTP"
     gene            complement(19451..19672)
                     /locus_tag="PLP50_05895"
     CDS             complement(19451..19672)
                     /locus_tag="PLP50_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51119.1"
                     /translation="MSLSDDQRKFYENTLRVTKKEISDLEANIQEELAKVKERLAELQ
                     NGQKAARQMYAAACLRLGIQNDLEESEES"
     gene            complement(19692..22451)
                     /locus_tag="PLP50_05900"
     CDS             complement(19692..22451)
                     /locus_tag="PLP50_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPA51120.1"
                     /translation="MAKTILLIEYEPRYLDRIKGYLAGKDFDLVVAKDGEDGLEAYRR
                     AKPDLVLISTVLPKLRTRDVIRGMQALGATPPILLMASGYKGKDKRADAAREGASGIL
                     EKPFSEEALLGEISSALGGGSAEPFAAAGAPPLLSSDDIFSDVISGVEAEEKARPGAP
                     VGTDPGINKKLEQTLSGILSRPGAKPAPAASAKPSPATVPAPRVEANPPSQPIPAPKP
                     KIDTAVDRMLTETLSGLRPVKPAEKPAPAPAPVAPPVAPPAPAPKPAPAPAPKPAPAP
                     KAKPAAGGPGSFGRYQLLEKIAAGGMAEVYKARMRGEEGFEKIVAIKRILPHMADNDD
                     FITMFVDEAKLAAQLTHNNIIHIYDLGKEDAYHYIAMEYVEGKDLRSILKQGSEKGYP
                     LPVELALFVASKVANALDYAHRRMGMDGKELNLVHRDVSPQNVLISFEGDIKLCDFGI
                     AKAATKVQQTQAGALKGKLQYMSPEQAWGKKVDRRTDIFSLGVVLYEMLTGERLFSGD
                     TDLTILEQVRDARSTPPSAKNGEVPPKVDQIVLKALAKNPQDRYQNASEMERDINSVL
                     YSFQPAPGPADLAIFLHRLVEATPVASDAQIDAAFAQAAAQAPAPEEKKKGKGLVISK
                     KEKVPEPVPVPEPVEVSQPAISVDEDAGKSRTGLFAGIGVAAVVLIAAAVFLTRGKKE
                     EPAPPPVATPTAVAEPAPATTEAPTAPEKVIDAKALEAELKKATAEEAKKLREAAQKA
                     AAEQQPRPATGAPATAAPAVSLAPSQPLQPPKPAATATPVPAPTRAPEPTPVPVRPTD
                     PPRAVEAPRPATPAPAAAAPAAAVREGDLVGQGPGVREPRLVKLGAFPRLPAQARRVT
                     GPAAVMALVDENGRVTSTRVVKSSGSPVADDAAVSAVKGAQIEPATKAGVRVKMWKTF
                     NITVK"
     gene            22529..23881
                     /locus_tag="PLP50_05905"
     CDS             22529..23881
                     /locus_tag="PLP50_05905"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPA51121.1"
                     /translation="MSLSTKFAAPERFGRRFGRAYAVHVLGCKVSQIEADGIARTLES
                     EGFEPARPGEAAEVVVLHACTVTARADRDALRLARRLRRENPAALLAVTGCLAERDPD
                     GLSLRPEIDVVAGHGSRLALPRLLEDAAAGLLPGKVAERTASPAEVLASPLAAGGTGR
                     SRTFLKIQDGCGRRCSFCIVPSLRGAERSAPASEVEDAIRALGEDGVPEVVLCGVHLA
                     AFGHDHGESLVGLLRRLEDRPPACRLRLSSLEPMEAGEALVDLVASSRVVAPHLHLPL
                     QSGSDAVLRRMRRGMTRARFRDLLLRAARANPRLHLATDVIAGFPGETDAEFAETEAL
                     LAELPLASLHVFPFSPRSGTAAAAQPAGASVPASLRTRRAARLRARAATLLRRFAAAH
                     DGRAADVVALRGDVGLTETYVEVALPPSGPPPASRFAALLALRPDGGLDARPAVPFSC
                     "
     gene            23936..24679
                     /locus_tag="PLP50_05910"
     CDS             23936..24679
                     /locus_tag="PLP50_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA51122.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGRLFTKLIKEITMSAKVGGGVVE
                     SNPRLRSAVLAAKKASMPSDNIDKAIKRGTGELEGVTYEEVVYEGYGPGGAAILIEAM
                     TDNKNRTTAEIRHLLSKYGGNLGATGSVAFQFSKKGYIAVEKSSAAEDALMEAALEAG
                     AEDFQAEDSDVYEIYTDLASFEAVKAQLEAKKFALQTAELQMIPATMVAVGDKAPALL
                     KLLDMLEDNDDVQKVWSSGDIDDQYLQDA"
     gene            24778..25314
                     /gene="ruvC"
                     /locus_tag="PLP50_05915"
     CDS             24778..25314
                     /gene="ruvC"
                     /locus_tag="PLP50_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPA51123.1"
                     /translation="MRILGVDPGSRAAGWAIVSFDGAPRLTAAGVLRPATSSSFAERL
                     LALHDALLTVIERERPDEAAVERVFSGVNPQSLITLGEARGVLLLALARSGLPVHEVT
                     PAEIKKTVTGTGQAGKEQVRRMVLALVGAPAGSSRLAFDAADAAAAALTHGFRTSAGR
                     RGLRSGPSSSPVRPRVAG"
     gene            25364..26257
                     /locus_tag="PLP50_05920"
     CDS             25364..26257
                     /locus_tag="PLP50_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA51124.1"
                     /translation="MSFQGSLKELPLADIVQLVAVSGKTGMFSLTRAAERGAVYIQNG
                     QITHAKVNDVEGEDAIYALALWNEGQFQFSAGVESETRTITRSNTNLLMEAARRSDEW
                     KILSKKIPTTEAVPQLVCREGLSEPVTLTPNEWLVVTGTDGVRTIEEIARGTRLSAFD
                     VAKTLYGLITAELVEIRTKAEARPAPPATAPAATAPAQASGPVVTTHSGVFNAVTRGD
                     ERRSLQILCTKVKQEAESAALAPGDPAIDRLYRAALGEVDKGRGLDVVRELIRSLEQT
                     VAALRGREASLAFREKMAPLL"
     gene            26505..27638
                     /locus_tag="PLP50_05925"
     CDS             26505..27638
                     /locus_tag="PLP50_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51125.1"
                     /translation="MRRAFVLLALAAVPLLAEEPAREEPAGIIPRIAVYVPEGEAEIR
                     LSKLVRSSLFETQFEYDFVSGDIAAFLRYKYYGSRQSFTLAAFDRVSFAALESLSLGY
                     DRVRGLSAFVRRPVGLYGRLVFLAEVDRFNVSGPKKPHESADDGQTNLFGKVGFQWGT
                     EDDDFGNRISGDPNDRVRNLFTVARAFAPGGRGLSLGVTYGVPILDYDYLKVEGEAIQ
                     AIDVGTSRLVARLHAGFFPHKPPSEGEPPPGGLPFRIPAGEYLSLGGRDALRGLRDAP
                     FGTDQLHVTLEAFVPVFVNRNSEFLKATWNTLYAVGYVGTGNAGVGRDVYTRLGDYRQ
                     DAGLGFEVAFRISRYRVFASALAAREIGGGGSPRFLMTFRTVN"
     gene            27765..29126
                     /locus_tag="PLP50_05930"
     CDS             27765..29126
                     /locus_tag="PLP50_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPA51126.1"
                     /translation="MRRMRTGLLLALAVALLAALPLSAQVAPTLTGETGLFGIQDADT
                     VPQGKFAFSLFYSMYDRTAASVPLLAPLGDDPLRYSTDKLGLSITFGLLPNWEASFTA
                     GQRYYSSDDRLWAGNIGGRNRVGEVDHDETDKLRIGTKYVLNRKDPLKVAIFGAAYIP
                     TQRKSDDAAMSSYRADWEWGFSGTYGIFTGQLAYLWTADHDDQYDETYEIANELSFSA
                     GVGVPLIPDRLKGIFEIHRVMYDGGTTEPPDLTDAVLGARFALGKSGLVAGAGIRANI
                     DRWVKYGSSPSNIGGLLQLSYAPQPEKVVEAPKVVPSPHVPPPVEPTPAPAPVLEPAP
                     APVVEAPPAPKPETSTTDEILFDAAKSRLTNIAKAILDGVALRLKNNLSATCAITGHT
                     DPGEKGDHAALAKARADAAKDYLVKRHGIDASRIQVDGRGDAEAADDATRNRRAVVTV
                     TFP"
     gene            29213..30361
                     /locus_tag="PLP50_05935"
     CDS             29213..30361
                     /locus_tag="PLP50_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPA51127.1"
                     /translation="MDSSGAFTTGTSPALGGVFRSRALVVLATAAVVGLLWAGKILFV
                     TIFFALFLSFALHPFVELLEKLRVPRGLAAFVVLLLLCALFAFFIVNALDQAQAFSRD
                     WPSYERKIQALTAQARGVFSRIEEGTRRLLSDGGASGVRSVRLEEGMLDSISRVVLQA
                     KTIFSLLLYTAAVPMLAFFMLKDREKYSGALTRILRRKGGRAASDFAVNVAHVLSGYV
                     LGELFVVLITACITTAGLLILKVPYSYVLGPLAGLCVLLPYVGVILSTTPAVFVAFLG
                     TDEPFLALKVVAFYSVVQFLEGNVLTPFIVGSRVRLHPLAVLVAFLFWGILWGLPGAI
                     LAVPLTAAVKVVGERFERFAPWAALLGDRTPDPEPEPDEPKPEPEAAG"
     gene            30366..31034
                     /locus_tag="PLP50_05940"
     CDS             30366..31034
                     /locus_tag="PLP50_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPA51128.1"
                     /translation="MCGRYVQTSPADALAARFGARLPEGLAWVPRWNVAPSTPAPVVR
                     ADGPGRELALLTWGLLPSWAKDAAGLRPINARAEGLAGKPMFRDALRRRRALVPADGF
                     YEWQASGPGKVPWHFRLKGGGAFAFAGLWERREAPGGPPLETFAIVTTEANDVVRPVH
                     ARMPVILDAEAEALWMREGALSPDEAARCLRPLDASRMEAWAVSSWVSSPARDGPRCV
                     EPAG"
     gene            31106..32353
                     /locus_tag="PLP50_05945"
     CDS             31106..32353
                     /locus_tag="PLP50_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51129.1"
                     /translation="MHPEHRAAFNRAFTSALARGQAEDLARRAGRAPGFRLAETPVFL
                     TRELEERLVSASHGILAQLSRPETVARLRRFVPPEWDAPGIDELPSIAQIDLAVARAA
                     DGSLEPKLVELQGFPSLSAFEVLQADAWEAALSEIPGLPAGGWSPYFSGLTRTTYLDL
                     LRRTVVAGHDPENVVLLDLHPEGQKTAVDFDATRRLLGVEAVCATEVEKEGRRLYRRR
                     GGRRVPIERIYNRVVVDELVRTGARLPFDFRDDLAVEWAPHPNWFWAFSKAALPLLDH
                     PAAPRTLTLDTLDALPPDLAKRWVLKPLFSFAGGGVNVEPTEADVAAVPAAERDRWCL
                     QEKIEYAPALITVDGGGVKVEIRMMFFRPGSRREFTLGLNLCRLSRGKMHGVDFNRDF
                     TWVGSSVALRPAAGWPGESSRGA"
     gene            32404..32964
                     /locus_tag="PLP50_05950"
     CDS             32404..32964
                     /locus_tag="PLP50_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPA51130.1"
                     /translation="MPQNLKGLKVLFLVAPKGFQVEELDKPRRVLEAAGAEVVVATTA
                     AGGATTQDGTSVDAVPIVELRPTDFAGAVVVGGPGSPEIWENGIAHKFFRMTAHDGKP
                     VGGLSLGVGLLAKAGLLEGKSATIWVTPEALRTLKDAGARYEKKPVAVAGGVVTADGP
                     SSAESFGNIFAELLDGSRSRSGRVTR"
     gene            complement(32983..34062)
                     /locus_tag="PLP50_05955"
     CDS             complement(32983..34062)
                     /locus_tag="PLP50_05955"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPA51131.1"
                     /translation="MSNAPRTTVIRSSGSYVPPQVVTNEMISRIMDTTDEWIVPRTGI
                     RERRFGAPGVGPAELAEHAARRALEKAGLAPKDIGLVVFATMTPDHYFPGSGGLLAAR
                     LGLTTTPALDIRMQCAGFLSGLQTADAFIRSGAFDRVLLVGAEVHSGLMPWPESVWDV
                     MLGRSPGPCDPYWYERATGMRDRAVLFGDGAGAMVLEANESGDGRGFLGIVLKTDGNH
                     WDKLYVPGGGCKSRPYFTPEMFESLGTIPIVEGRQVFRLASQLMPEAVREVLAGCGRS
                     LEELDLLLMHQANLRINESVQKVLGLPDEKVFNNIQKYGNTTAATLPLVYDEAVAAGR
                     IGPGSLVGFTALGAGLHWGAALARI"
     gene            complement(34078..34374)
                     /locus_tag="PLP50_05960"
     CDS             complement(34078..34374)
                     /locus_tag="PLP50_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPA51132.1"
                     /translation="MDTEEDVPVVLPLEDAIDLHAFRPAETASVVEEYLAEAAVRGFD
                     EVRIVHGRGVGTQRRIVRAVLARSPLVVEFRDAPPERGGWGATVARLRPGGAVR"
     gene            34453..35361
                     /locus_tag="PLP50_05965"
     CDS             34453..35361
                     /locus_tag="PLP50_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPA51133.1"
                     /translation="MKHRFVVAAGLLAALASSTALAAKPRVAVIEFKDKTQHYSWYRA
                     GEAAQDMLVTELVKSGKYRVIEREQLAAIMQEKNLSLSGEIDPKTAVKAGKLLGVEYI
                     VTGALTELGVADRGARVPGGLFGRLPSVNVGSQKMDAAIDARMINTTTGEIVWADSAK
                     ESTSDAKVFVAGAGGGVEDRRKLDRVLRPVVVKLAESMGKVGVSTSGLGGASDASGIA
                     GKIAKVEGGSVFLNVGAEAGVKEGDTFDVYRVGNVIKDPDTGEVLGSDETKVGRVKIT
                     KVMGARLSTASIVSGSGFKPGDMIKN"
     gene            complement(35437..37557)
                     /locus_tag="PLP50_05970"
     CDS             complement(35437..37557)
                     /locus_tag="PLP50_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA51134.1"
                     /translation="MTPGTLLGRYRLLERAGAGGMSEVWRAEDQTLHRTVAVKVIHDP
                     IAADPTFAERFLREARLVAGLEHPNILAVYDFGTAPLEGKEVSYLVMPLVSGGSLKER
                     ITGPVAPAQAVPWLAAIAAALDHAHAKGILHRDVKPGNVLLDASGRPLLADFGLARSA
                     DSVSGLTATGTVMGTPSYMAPEQAMGKALDARADQYALACIAFEMLTGRVPFKADTPL
                     GVLHQHVSVPPEPVTSFVGGLPPAIDDVLSRALAKDAWRRYESCTDLVSALAAVFGLS
                     LGAAAAASAPVRAVPPPPPGAPTIHGAPTVLEAGGARVAADLPTVATAGAGVVPSAGI
                     SSPDTAMAAAASMPPPPPVAPARAGKSPALLVAATVAGLVVLGGGGWLAYSRFGPGQT
                     APEEKPAALASLAPGATSATEPAPAETVPTGPATDSFGSAGSGAPTETGPGTAPQAAS
                     TPLAASSVEESGPMGQVVETRPGPAATNSEDVVGKGQRGSSDTGGVLSGLESGSRDTG
                     AVVSADPSLPRREALVDGGQWSLDTRLEEAYRALDTTRSKGTLTREALAEAGGIAREI
                     ASTSDEPEARFIDLYTRAGSAFLNGENATAWQQLHEAFRVDQSGAAGGRVLRFVRRVM
                     KGKGMNPGRDANWILGLAFGDVRGDLDEELAAAAERAPGNRAVRVARALRAVEVRDGR
                     RAGALLREACGDGIEEACELLGRR"
     gene            complement(37671..40163)
                     /locus_tag="PLP50_05975"
     CDS             complement(37671..40163)
                     /locus_tag="PLP50_05975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA51135.1"
                     /translation="MPTARDLALLKAFDAVLAEGRFLDESIGAVLETALRFFDAVAVA
                     LLPAGGAPPMSRAGSSIVSAAAEQRLGRALETHLSEGREGRVAESGLVFLCAAVKTGE
                     QVSGAFGVALAQSAGKGMEVDDGIRSFARILGRILERERSVATLVKRREEAVALFELA
                     SGALHTLNADEVVRLTAASLQRQLEFEQVRAFRYHPEKGEVESVLAQGTSPGTGGGFV
                     PGQRVPIDNDGILVRALAAHGPAFDEDDADDSKGPRRKRRLAIPIEQGETVFGFLTMS
                     RRGAFVLTAQEMRLVQELARLAAGALERARLIELERRTAEHVTFVNRVHAALSGLNDL
                     EAILAKTVEETGAGLGLDLCAVQVRSFEGGRPPSSVWTKDGASVPGVEGTIPRALVDR
                     LAHPEAAVLLSDVALDADGPALVPMPNAAKELPRPLGLVAVPLVSHGEFLGVLSGVGA
                     GRPWAFDPPLLRAFQAVAAEASHAVTSARLLQRERDSYRFLDRLREVGRSFATTFDGP
                     RIKHALCEHAIALLAGESALFWDADAQTKSLRVLAQGGSDPSAEAPRHVPTESTGHPV
                     VRAWAEKALVLVDEAEIGALYPAAAGSPGLRAIAAPLLFQDEQIGVLTFAFRSPGGPW
                     PAELAGRLSLLADTAAVALHNSRLMKIIEQQTERDGATGLYNQGAIVKRLESELKRAE
                     RSGQPLSVAHVRIDGLGEASQRFGMPYGDALLPKIAAQLVRATRSVNIVGRGKGDRFW
                     ILIFDADKPIAQRAAEAIAKNFASFRDPRLDATGLRFSLTLGLAAYPEDAFDTASLLS
                     RAEEALDDASRSGPGSIALWGALAGADVAEEL"
     gene            complement(40239..40718)
                     /locus_tag="PLP50_05980"
     CDS             complement(40239..40718)
                     /locus_tag="PLP50_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPA51136.1"
                     /translation="MTLREALTHPWLTVRTQIALGLFFIVAALPKIADPPGFAHMVYN
                     YKMLPGPLVNVMALVLPWAELLMGLALVAGIWRKTAAALVGALLVVFIVAISVNLLRG
                     NAIDCGCFDVTAANLTREERFRDMWMVIFRDVGMLFLVAQGLLGARRVETDGEASAI"
     gene            complement(40715..41467)
                     /locus_tag="PLP50_05985"
     CDS             complement(40715..41467)
                     /locus_tag="PLP50_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA51137.1"
                     /translation="MNLRRFLAEAGALAVLAIACALVSNAFAAKERRLELPGDYPAAF
                     SVPKPPAPVPFERHPAPAADEPAPPASAAPAVPDRPAPSAPAPAPGSRRPAADREPAP
                     PPQERPAASSPSSPAAPDHVAEVLARFPPHGQPGVEITGEAAAWLHARGALFLDARRS
                     KDFVEKHVAGARSFPIWEAAVVKERVEELVAEGRDQTLPVVLYCTGGDCEDSHMLAQT
                     LFGAGFENLLVYRDGFPDWLSRGGAVATGRGR"
     gene            41637..>41766
                     /locus_tag="PLP50_05990"
     CDS             41637..>41766
                     /locus_tag="PLP50_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HPA51138.1"
                     /translation="MSENTLTIRDNRTGKEYVVPVENDTIKAMDLRKIKVNPEDFGL"
BASE COUNT         5694 a        14976 c        15261 g         5835 t
ORIGIN      
        1 gctactgcac gctctacggc gacatggccg gcgggctcgc gccgatctcg gacctgccga
       61 agatgcgggt ctacgagctc tcccggcacc tgaaccggcg cgccgggcgt gagctgatcc
      121 ccgcctcgac gctcgagaag gcgccctcgg ccgagctccg gccgaaccag accgaccagg
      181 actcgctccc cccgtacgcg ctcctcgacc ggatcctgga gggcctggtc gccgggaacc
      241 tccctcccgc gaaggtggct cgggagaccg gcgcccccgt ggcgctcgtc gcgggcctcg
      301 cccggcaaat cgaccgggcc gagtacaagc ggcagcaggc cgcaccgggc ctgaaggtct
      361 cgacgaaagc cttcggcagc ggccggcgca tcccgatcgc gcaggtcccc gggacgtgaa
      421 gtacgcgggc gacctgcccc gttcccgata cccgtggaag gagatcccgg gctcctcgca
      481 cgcggtcctc ctcgggcgag tcgccgcgct cggcgactct ctctcggtcc tggacgtcgg
      541 cttcggggcc gggcacctcg cccgccgcat ccgcccccgc tgccgctacc tcgccggaat
      601 cgagctcgac cccgaggcgg cccgggaggg ctcggccttc ttcgacgacc ccgtcctcga
      661 cgacgtcgtc cgcggcctcg ccggcccctg gcgcgaggcg ttcgacgtcg tcgtcgcggc
      721 cgacatcctc gagcacctcc ccgagccggg gcaggcgctc gacctcctgc ggccgctcct
      781 ccgcgaggag ggcctcctcc tcgtctcgct cccgaacgtc gcgaacgtca cggtccgcct
      841 cgcgctcctc ctcggacgct tcccgcggac ccagcgcggc atcctggacc ggacgcacct
      901 cgggttcttc acccgccgga cgggacggga gctcctcgag gctcacggtt accgggtcgt
      961 ccgcgagacc ccgaccgcga tgccggtcga gctcgccttc cccgtcctgg gccgaccgcc
     1021 cctctccgct cccgtccgcg ccgccgcgac gctcctcgca cgcgcctggc ccggcatgtt
     1081 cggctaccag ttcgtctacg aagcgaggcc cgcttgaccc gaccaccggt cctgacactc
     1141 gtcgtccccg cctacaacga ggagaagcgc cttcccgtct ccctcgcccg gatctccgag
     1201 tgggtcctgt cgcgcacgcc gcagctctcc gtcgaggtcc tcgtcgtcga cgacggctcc
     1261 tcggaccgga ccgccgcggt cgccgagaag accgcggccg ggctcgggct cgacttccgc
     1321 gtcctccggc tgctccggaa ccgcgggaaa ggagcggcgg tccgcgaggg ggtcctcgcc
     1381 gccgccgggg agcaggtcct cgtcacggac gccgaccttt ccaccccgat cgaggaggtc
     1441 gacaagctcc tcgccgctgg cgcccccgtg gcgatcggct cccgcgccgt cgacgagacg
     1501 ctcgtgaagc agaggcagtc gctcttccgc gtcgcgagcg ggaagctctt caaccttctc
     1561 gtccgcctcc tcgccgtctc gggaatccgc gacacccagt gcggcttcaa gctcttccgc
     1621 gccgacgccg cgcgggagat cttcagccgc gccaccgtcg accgcttcgc gttcgacgtc
     1681 gaggcgctcc tcctcgcgcg ccggctcggc tacccgatcg cggaggtgcc ggtcctctgg
     1741 ttcaactcgc ccgacacccg cgtcggcctc gggggaggcc tcgaggcgtt cgcggccctc
     1801 ttccggatcc ggcgccgcgt cgcccggacg ctgcgcgcgc gccctccggc cccgcgcgcc
     1861 tcctgacgac cggggtggtt ccctctccgc gccgcggccg atacgattcc cctccgcacg
     1921 gaggtgaacc atgaagccga aagtcggtgt cgtcctctcg ggctgcggcg tctacgacgg
     1981 cgccgaggtc cacgagtcgg tcctgacgct cctcgcgctc gaccgcgccg gcgccgaggc
     2041 ggtctgcctc gcgcccgacg tcgcgcagcg gcacgtcgtg aaccacctga ccggccagcc
     2101 ggcggagggc gagagccgca acgtcctcgt cgaggcggcc cgcatcgcgc gcggtcaggt
     2161 gaagagcctc gccggcttcg acccggcccc gctcgacgcc ctcgtcctgc ccggaggctt
     2221 cggtgcggcg aagaacctct gcgacttcgc gttccgcggc gccgactgcg aggttcatcc
     2281 cgacgtcgca agcgtcgtcc gggccgtcca cgccgccggc aagccggtcg gcgccgtctg
     2341 catcgcgccc gtgatcctcg cgaagctcct cggcgccgag aagccgaagc tgacgatcgg
     2401 aaccgacgcg gggacggccg cggcgatcgg gaagatgggg ggcgagcacg tctcctgcgg
     2461 gggcggcggc gccgtcgtcg acgccgaacg gcgcctcgtc acgacccccg cctacatgct
     2521 cgacagcccg atctcccagg tctcggccgg aatcgagaag ctcgtcgggg agctcctctc
     2581 gatggtccgc ggctgagggc gcctcagccg gtcttctcga tccagaaggc gcagccgtcg
     2641 aaccggtcgc gcgggcagcc gtacgcatcg gcgatcgcgt cgaggccgag ccgctcgcgg
     2701 ttccgcgaga cccagtaggc ggggagcgtc acccggtacc ggccctccgc cccgaggagc
     2761 caggcggcga ggacgtactg ctcgctgtag tagcgctcgg cccactcctc cgggtagtcg
     2821 tcgggaagga agatgtcgtg gattccgatc gccacgccgg tcgggacccg cgccaggacg
     2881 tcgaggaaga acgccgtcac gtcgctgttc atgaagcagc gatggctccc gtcgaagaag
     2941 acgacgtctc ccgggcgaag gccgtcgaag agcgcgaggt cgaccgtctc gagcggctcc
     3001 cggatcaccg tgtcgcagag ggcgtcgcac tcggcgcggg ggaagggatc gaccgagacg
     3061 attcgcgcct tcagctcgag agccccgatg agatgccgga ggaacttcgt ggagttgccc
     3121 gagccgatct cgaggacctg ccgcgtctcc ggcccgagga acgctgcgaa cgccacgccg
     3181 tcgagcccgg ccatcatcgc gttccgccag cgcgggcgcg gatcggcgtc gtccgccgtc
     3241 tccgggatcc cgcttccgcc gtggcgtcgg acgagctcct ctccccgttc ggagatccgg
     3301 cgaacggtct cggtccagac cggctcgtcg cgctcgatca gccgccgaac gccggggtgg
     3361 ctccccgctt ccgccgtcca ccggggccgg ttggccgccg aggtcgggta gtcgaaggag
     3421 atcgtcgtcc cgcccatcgc cggggccggc tgcggtcctg accgcagcgc cagcgccgcg
     3481 aatcggccat ccgcgtcgcg caggaccggc gcgagccgat cgtggacctg gtcggcgacg
     3541 atccgaccga gcttccgcgg gagccatccg agcacgctca tgcggcgccg atactagcaa
     3601 cccgggtgcg gcggccggcc cggctccccg ggtcggccgt cgctctgcgg gctgcggtct
     3661 ccgaagggcc gggaccgcgg gtcagtcgaa gtagccgctg acgtcgacga tgacgtggac
     3721 cgtcccgccg accgggaccg tcgcctccag gcgcgcccgg cccgtgccgt cgtccgaaag
     3781 cttgatgatc acgttggacg cccgcgtctt gccagcgacg aagtggacga cgctcgtccc
     3841 cgtcgaggcc gcgtccgccg ggtagacgga gacgtgaccc gggagcgtcg gctgcgtcac
     3901 ggtgacgttc gtggcgaggg cccgggcggc cgccggcacg ccgcacgcgc cgtggaacgc
     3961 gacctccctc ggcacgccgt ccgtcatcgc cgggccgtcc tcgcgcgtgt cgaggacgcg
     4021 gcacggcgcg agcgggtaga agcgcccgcc cgtgcaggtc gtctggaacg tggcgtcctc
     4081 gccgttcgtc gttccggccg cgctcgtggc cgtggtgcgg tagtggtagg tcgtgcaggg
     4141 ggcgaggtcc gacagggccg cggagacggc ctgcgcagcc gtcccggcac cgatggcctg
     4201 ccccggcgtc gtctgtccgt acgacgtcgt cgtcccccat tcgaaggaaa cggtcgtcgc
     4261 gactccgttc gggttcaccg tcccgttcag cgtcgcgccg gtcgtcgtca cgccgctcgc
     4321 cgcgccggtc gacacgaccg gtgccggaca gctcgtctcg aacgtggcgt ccgacccgtt
     4381 cgtcgttccc gcggagctcg tcgccgtggc gcggtagtgg taggtcgtgc aggccgtgag
     4441 ccccgtcagg ctcgccgaca ggggcagcgc cgccgagccg gtgcccatcg actgccccgc
     4501 ggtcgtctgc ccgtacgacg tcgtcagccc ccactcgaac gaggccgtcg tcgcgaggcc
     4561 gttcgggttg acggtgccgt tcagcgttgc gccggtcgtc gtcacgtcgc tcgccgcgcc
     4621 ggtcgtcacg accggggccg gacagctcgt ctcgaacgtg gcgtccgacc cgttcgtcgt
     4681 tcccgcggag ctcgtcgccg tggcgcggta gtggtaggtc gtgcaggccg tgagccccgt
     4741 caggctcgcc gacaggggca gcgccgccga gccggagccc atcgactgcc ccgcggtcgt
     4801 ctgcccgtag gacgtcgtca gcccccactc gaacgaggcc gtcgtcgcga ggccgttcgg
     4861 attgacgatg ccgttcagcg tcgcgccggt cgtcgtcacg ccgctcgccg cgccggtcgc
     4921 gacggtcggc gcgggctcca cggcggaaga gtcccagtgg agctcgacga actcgttggc
     4981 gaaggcgctc gcgtcgctga actcgttgat gacgagaccg gtcccgcagc ccgccccggc
     5041 gccggtgccg ggtgtggccg tggagagggc cccggccgtc cagctccccg ccgagcccgc
     5101 gggctggcag gggtcgttgc gccggacgct ctggaacgtc gccgggagcg cgatcgtcgg
     5161 accgtcgacg atgacgccgg aggcgttctt cagcgtgtac gtctctccgc cgttgatcat
     5221 cgggatcgtc tcgccggaat tcaggtagac gacgtcaggc ccaagggtga cgccccaggc
     5281 cgtctcgaac gccgtcttct ccacgttgcg cccgatcacg acgtagccgc ccgccgggac
     5341 ggtcgtcccc gcgggcagcg tgtaggtctg ggtcgagttc gcctggacga gctgccagcc
     5401 gccgatgtcg agcgtcgtgg cgacggccgc cgtcgtcacc gtcccgtccg aggaccacgc
     5461 cggcccgttc ccgcaggggt ccgtcgagcc ggcacggaag tggtactccg tcgagggcga
     5521 aaggccggtc accgtgagcg agtgcgcggt gacgggcgtg gcgtcgtacg ccgaggagcc
     5581 gtagccggcc gtgaggccgt actcgaggcg gctgtccgac ggctcgtcgg tggtccaggc
     5641 gacggtgaag gacgacgaga cggccgacga ggccgaaggc ccggccgtca ggaccggcgc
     5701 gaggccgtcg tcggcccggt cggcggcgga gatcgacgtg ttgccggtgc tcgtctcggc
     5761 ctcgtgattg aaggcctcga cggcgtaggc gtacgagacg ccggtcgtcc cggacgtgtc
     5821 cgtgcacgag gtcgcggatg cggtcagggg gcccgcgcag ccgccagagg tgatcgcaga
     5881 gccatcccgg aggacgtgga acccgcgatc acagccggcc gtgcagtcgt cgttccagga
     5941 ggacggggcg ttccacgtca ccccgacgcc gccagtcgca cagaggttcg ggtccgtcgc
     6001 cgactggatt ccggagaaga cgatgcccgg gttgccgccg cagatcccct ggaagacgca
     6061 ctccacccac tccgggtggt cgacgaatgg gttccggttc ccctggaagg acgcgatgac
     6121 gtcgttccgc gcccgctcgt cgtcgtcgac ggggtccgcg aggtgccact ggaggaggac
     6181 cgtgaggcgg cccatgtagg cggtgccccc ggaggtcccg gtgatctgcg agaggttgtc
     6241 ggtgaggatc agctgcggct cggacttccc gttgatggcg tgagtgcccc cctcgtaccg
     6301 gacgtccatg tagagctgcg cccgggcgag atcgccgcgg cgatggttcc agacctcgta
     6361 gatccggctc gacggggagg accagttgga gttgcccggg tacgtcccgc taccgccgcc
     6421 gaacccgtag ttgagatcgg tctcgtcctc cgcgcagccg ctgttgcacg tcccgaacgg
     6481 gtcgttcccg cggttcccgt tgtattccgt gttcgtcgcg aacagatggt ggcagtcggt
     6541 gtacggtaca tccccgttgc tcgggaagcc gtacgagttc ggccacgagt gctcgcggtt
     6601 gtagatcccc gtccccccgg cgatcttggt gtagcgagcg ttcttgtact tgtccaggat
     6661 catccccgcg tcgaccgggt cctgctcggc caggttcagg atgtcccagg tgtcggtggt
     6721 gtcggcggtg tacggaaacc agatgtggtt ctcgatcgca gcgtggatcg tcgacctcag
     6781 ggccgcctgg ctcgaggcgt cgacccccgc gtagtagtcc tcgaggtgga cgcgcgtgac
     6841 cgaagccggc gtgttccagg cctcccccga gacgagcgtc cccgagaagc cggggagccc
     6901 ggctccgtcg aagtcgttcc gcttccagtc cgtcacggag ttcgtgtcga cgtagtacgg
     6961 gaagcgcgac gcaccgcccg gggccgtcgc gtttccgtcg aagcccggcc cgaggacggg
     7021 ggccgtgtac gtcctggcgc ccgccgtacc gtcggagaac gccacgccgt cctgaatcga
     7081 ggaccacggg ttcgcgtcga ggactccgtc gtttccggag tccagatcgt caccgaccaa
     7141 gcccgtgaat cccgagacga gaaggaccgt gaacaccggg ctctcgagaa gcggggtgag
     7201 gcttcccgag gaccagaacc cggcgccgtt cgccgttccg ggcgtcacga cgttcaggat
     7261 ctgtcccggg ttcgagctcc cgtcgagaac gacgacccgg aacgaagaga ggtccgccga
     7321 cgggtccgcc cagagctcga cgtactcgtg cgtgtccggg ctgccgacgt ggtcgaagac
     7381 gacctccgtg atcttctgcc ccgccgccgg tccggcgagg acggcggcga ttgcgaacgc
     7441 gagggtcgcg cgaagagcgg tgaatcgcgg gacggtcgaa agtcccgagg aacgtcgttc
     7501 ggttgtcctg ggcatgggtc gttacctcgc acgggccggg aaggccaaag aggagattct
     7561 agtcccttcc actgctccgg gagcactgcg aaggtcgcct gttccgtcgc gagggtcccg
     7621 ggcccgctcc cggtcccccg gaaagcgcct cacccgggca gcctggaccc gcacggcgac
     7681 ccctccggcg cacgtcgcgt tcgaagcggc ttctcttccg cgcgccggtg acgcccctca
     7741 cgcgaccgcc tcctgagacg ctgtcaattc gccgaatgtc gtaatctgag cgatgccgcg
     7801 acagtcacgg cgcgaggggc ttcccgacga atgcggcgga ggtgatccat ggcgcagctc
     7861 tcacgttcgg ctcgatccgt gcgagtgggc agagtcttcg ctgtgctcgc ggccatcgcg
     7921 ggcttcgtct cgtcggaggc ccgcgcgacg gtgcagccga ccgggtcgat ggcggggccg
     7981 cggtcggagc acacggcgac gttcctcggc accgggaagg tgctcgtcgc gggaggggcg
     8041 gactcgacgg gccgtctcgc gacggcggag ctgtacgacc cggcgacggg gtcgttcgcg
     8101 gcgacggcgt cgatgccaga ggagcggcac gggcacacgg cgacgatgct gccgaccggc
     8161 aagatcctgg tggccggcgg gaacggcgac gcgggagcgc tcgcgacgtg ccggctctac
     8221 gacccgcgga cgggaacgtg gacgtcgacg gggtcgatgg cgacggcgcg acgggagcac
     8281 acggcgacgc tgctcgcgaa cggcaaggtc ctcgtggccg gcgggcagaa cggcacggcg
     8341 cctggctaca ccgcgctggc gagcgcggag ctttacgacc cggcgctcgg gacgtggacg
     8401 gcgacggggg ctctcgggag cgcgcgggcc gggcacgcgg cggcgctgct cgcggacggg
     8461 aaggtcctcg tggcgggcgg gcagacgagg tggatcccgc tgccggcggt ctacacggcg
     8521 acggcgttcc tctacgaccc gacggcggga acgtggagcg cgacggggtc gatgacggcg
     8581 gctcgcaggt cgttcacgct gacgcggatt ccgggcgggc tcgtcgtggc ggcgggaggg
     8641 cggaacgcga gctatctctc gagtgcggag cggtacaacg tctcggcagg gacgtgggga
     8701 agcgccggag ccctcggcgc ggcgcggagc ggccacgcgg cggcgctgct gccggacgcg
     8761 cgtgtcctcc tcgcgggcgg aagcgacggg acgggcccgg ccggggcgat gctggagtac
     8821 agcccgggga cgaacacctg ggcggaggtg gagacgggcg gggcgcgggt cgaccacacg
     8881 gcaacgctcc tgccgggcgg gcgcgtcctc gtggtcggcg gagagcaatc cgggtggctc
     8941 tcggacgccg agatcgtcga ccggatgaac ccggcgtgga cgacggcggc ggcctggggg
     9001 caggccgacg ggaccgcgac cctcctgccg agcgggaagg tcctcctcct cgggaaagcc
     9061 gtcttcggcg acctgtcgcg tggcgtgctc ttcgacccgg agacggaaac cgcggtcagc
     9121 acgacgggag cgatggtggc ccgaaggtcc aagcacacgg cgacgctcct cgcggacggc
     9181 gacgtcctga tcgtcggcgg ggtggacggc tccggagcgc tcgtcgcgca ggacgagatc
     9241 tacgacccgg actccgacac gttcaccgcg ctcgccgtgc cgctcccgac gcgaagggcc
     9301 gagcacgccg cggccctcct ccccgacggc cgggtcctcg tcgcgggcgg gcaggcctcg
     9361 agcttcctcg cgccgtacat ctactccggc ggagtctgga ccgtcgtcaa cgaagagcat
     9421 gggtacgacg gctggggggt tcgcctgatg gtcctcgcga acgggcacgt cctcgtcagg
     9481 agcgggaatc tcctgctcca gttcgacccg ttcccggggg cgctcaacac gcgctggcgg
     9541 accctggaca tctcgtcagg caacgccttc ggagagccgg tcctccttcc gagcggcgac
     9601 gtcctcttcg tccaggggaa ccccgtcccg cgctacacgc tctgggacgt cgagtcgtcg
     9661 agcttccggg aacccttccc cgcgatccct cccgccatcc ccctgcggtt cggtttccgc
     9721 gccctggccc tgcccgacgg ccgagtcctc gtcaccggcg gctacggcgg cgactcgggc
     9781 gtcgacccgc tggcgcacgc cgcggtcctc gacccggcga ctggcgtgac gacgcccctc
     9841 ccctcgatgg cggcggagcg ctccaaccac acgatgacgc ttctcccgaa cggacgggtc
     9901 ctcgtcgcgg gcggggtcat cgggagctcg acgacgatcg aggcgaccgt cgagacgctc
     9961 aggctgtggc ccggcctcgc ggaatcctcg cgccccgtca tcacctcgtg gcccgcttcg
    10021 ctgtcgctcc cgggctcgtt cgtcctgaac gggaccggct tcctcggaat cggcgaggcg
    10081 tccggcggga acggcagtgc gagctcgtcc gtcggcttcc cgtccgtcct cctgcgaagg
    10141 atcgaaggag gcttctcacg ctggatcccg tcgttcgagg gcgcagcctt cggagcgacg
    10201 gccttcacct cctccgaggt ctccgggatc ccgggaggcc actacctcgc gacgctcttc
    10261 gcgagcggca tcccctcgaa gccgaaggtc gtcgtggtga gcccgtgcga gatctacgtg
    10321 ggcgtcatgg ggagcacctc ggacgcctcc gtctgtgtcg gcgacgacct ccgcctggag
    10381 gggcgccttc tctccaacgt gacgaacgtg gtgtggacgt ggacgctccc ggacgggagc
    10441 acgtcgacgg accggctcct gacgatcccg gacgcgcagg tcgaggacgg gggcttctac
    10501 acggtcgtcg cgtcgaggcc cggctgcgtc ggccccgtcg acacggtgga ggtccgcgtc
    10561 gccccgcccg gccagatgcc gatcatcacc gcgccgttca cgctccttca cgctcaggag
    10621 ggagaggcgt ccgtggagtg gcagctcggg agcagctgga cgtggaccct ctcgggcggg
    10681 tcgatcctgc gcggccaggg gacgaaccgg atccgcttcc gggcggcgac ggacggagcc
    10741 tccatgacga tcaccgtcgt cgagtcggtc ggggagagct gcgaccagcc gccgtcgatg
    10801 gccgtggtcg agctcatggc tcccccggtg ctctactcgc cggtgacgcc ctgccgtctc
    10861 ttcgacacgc ggaacgacac cggacccgct gcggcggccc cggcgctcgg ccccggggag
    10921 acgcgggtct tcagtgtggg aacgcgctgc ggcctcacct cctcggcggt ccgcgcgctc
    10981 gcggtgaacc agacggtgac gcagcccgcg gccaccggag acctcgtcct ctatcgcggc
    11041 gacgtcacca ccatcccggt cgccagcagc gtcagcttca ccgcgggaaa gacgagggcc
    11101 aacaacggga tcctcgagct ctccaagttc ggggacggga cgttccgggt ccacaatcgg
    11161 tccacgggca ccgtccactt catcctcgac gtgagcggag tgttcaagta ggcgcagaac
    11221 ccgctgaagc tcgggggagg cggatcgcgc catccccgga cttcagccgc cgggacccgg
    11281 gcgggcccgg gtcccctttt cctcgtcgct cgcggagcga gcctcctctc cgccgcggag
    11341 ggggccgagg gggaacgggg gaggcgggtc gcgcctcccc cgaaccttac ttgtcgggac
    11401 ccggacggga ccgggtcccc tattcctcgt cgctcgcgga gcgagcctcc tctccggcgc
    11461 ggggaggttc gggggacccg gacgggaccg ggtcccccga gaacgctagt agatcgggaa
    11521 cgccgaggtc agctccttga ccttcgcctt gacggcgacc ttgaccgcct catcctccgg
    11581 cgccgcgagg acctcgccga tccagccggc gatcgtcttc atctcggcaa cgcccatgcc
    11641 gcgcgtcgtg acggcggggg cgccgagacg aaggcccgag gtgacgagag gcgggttcgg
    11701 gtcgaacggg atcgcgttct tgttgaccgt gatcccggcg tggtcgagcg ccttctcggc
    11761 gacctttccg gtgagctgct tcgggccgag atcgacgagg aagaggtggt tgtcggtgcc
    11821 gccggagacg atccggaagc cctgcccggc gaccgcttca gcaaacgcct tcgcgttcgt
    11881 caggacgcgc ccctggtact ccttgaactc gggcgcgagc gcctcgagga aggagacggc
    11941 cttcgcggcg atgacgtgct cgagcggccc gccctgcagg cccgggaaga ccgccttgtc
    12001 gatctccttg gcccactcgg gcgtgcagag gacgaggccg ccacgcggcc cgcggagcgt
    12061 cttgtgcgtc gtcgaggtga cgaagtgggc gtggccgacc ggcgaagggt ggagacccgc
    12121 cgcgacgagg ccggcgatgt gggccatgtc gaccatcagg agcgcgccga cggagtcggc
    12181 gacctgccgg aagcggggga agtcgaggac gcgcgggtag gccgaggcgc cggcgatgat
    12241 gagcttcggc ttctcggcct cggcgatccg ggcgagctcg tcgtagtcga tcgtctcggt
    12301 ctcgcgcgtg acgccgtagg agacgacgcg gtactgcttg ccggagatcg agagcgggtg
    12361 gccgtgcgtg aggtgtccgc cgtgagcgag cgacatcccg aggagggtgt ctcccggctt
    12421 caggcaggcg aggtagacgg cggtgttcgc ctgcgagccg gagtgcggct ggacgttcgc
    12481 gtggatcgcg tcggggttcg cgggcgagaa gagcttcttc gcgcgggcga tcgcgagcga
    12541 ctccgcgacg tcgacgttct cgcagccgcc gtagtagcgc cggcccgggt agccttcggc
    12601 gtacttgttc gtcaggacgg agcccatcgc ctcgaggacg gcgcgggagg cgtagttctc
    12661 ggaggcgatc agctcgaggc cgtcgcgctg gcgggcgaac tcggcctcga cggccgcggc
    12721 gatctcgggg tcgacggtgg agagggaccg ccccatcagg cggggagtcg gggcttcagt
    12781 cgtcgtcatc gggatgctcc ttgggcgctc ggcggggggg cgtcgagctt gtcgacgcgc
    12841 cgctggtgcc gcccgccggc gaacggtgtg gagaggaagg cctcgaggat cgccgtcgcg
    12901 agcgggacgg cggtgacgcg ggcgccgagg ccgaggacgt tcgcgtcgtt gtgctcgcgc
    12961 atcagctgcg cggagaagag gtcggtcgcg acgccggccc gtgcgccccg cacgcggttc
    13021 gccgcgatcg agatgccgat cccggtgccg cagacgacga cgccgcggtc ggcgcggccg
    13081 gacgcgacgg cctcgccgac cgcgtagccg aagtcggggt agtcgacgga ggcggtggaa
    13141 gaggtcccga ggtcgatcac ctcgtggccc agctccgcgg tccgacgggc cagaagggtc
    13201 ttgagctcga agccggcgtg gtcggcgccg aaggcgatgc gcatccgtcg atcctacccc
    13261 gcgggcgtcc ccgccggccg cctcgggtag ggggcgaaga gggcgtcgta ctcggcgagc
    13321 gcgtagctgt cggtcatccc cgcgatgtgg tcggcgatac agcggaggac ggccggttcc
    13381 tcgcgccagc gcccggcgat gaccccatcg accttcttga gcgggacctc gcggaggaac
    13441 gggaggccgg agagctccgc gacgcgcgcg aggacggcgt cgggcagaag gcgcgggttc
    13501 ttcgcgtagg cgtcgaagag gcggttgacg acgagctgcg cccgggcgtc gtgctgcgag
    13561 acggcctggc tgtggatgat gtggcgatag acgaactcct tcagctcgag gtaccgcttc
    13621 tccccttcgc gcgagggggc cacgagggag ccggggagct tccggcggtg cgcggcgagc
    13681 tcggcccgcg tcgtcacctt ctgcgccgtc agccacttct cgatcgccgc gcgcgaggcg
    13741 acgacgaggt cggtgacgag gtggtggatc aggccgcgga tcaggagcgc ccgcttcacg
    13801 gccgggtcgg aagcgtcgcg ccaggcggcg ccgagcgccg ccacgatctc cctcgcgagc
    13861 gggatctcct cggccttccc gatcccgacg aggccgaggc cgtcctccag gtcgtgcgcc
    13921 tgctgggcga tctcgtccgc ccagcccacc gcctgcccct cgagatggca gcccgagccg
    13981 aggtggaggc cgtccgggtc gaggacgggg aacgggaagt cgcgcttcca ggtggtgtgc
    14041 ttgaggatcc cctcgcgcgt ctcgtcggtc aggttcagcc ccggttcctc gtaccgcttc
    14101 tcgaggaggt cgacgacacg gaccgactgg tagttgtgct tgaacgcgcc gacggcggcg
    14161 accgccttcg gcgagagcga gacgctcggc tcgcccccgg tcaggatgcg gttcagcgcc
    14221 ttctcgccgg agtggccgaa cggcgtgtgc ccgaggtcgt gcgcgagggc gcacgactcg
    14281 gcgaggtcct cgttgaggcc cagcgtccgt gcgacggtcc gcgcgacctg cgtcacctcg
    14341 agcgtgtgcg tcagtcgcgt ccggtagtgg tccccctcga aggggatgaa gacctgggtc
    14401 ttgtgcttca gccggcggaa cgccttcgag tggacgatcc ggtcgcggtc gcgctggaac
    14461 tccgtccggt agtcgtggcc gtccgcctcg gccgggatct cccggcggcg cgtcgaggcc
    14521 gcggcccgcg aggcatagat cgcgagccgc tcgtcctcga acgcctcgag ctcgcgcttc
    14581 gtgaaggcga cggcccgcgg aggcctccct gcgttcatcc gaacctccgg gcgcccgccc
    14641 gccggcggct caggcgtccc ggcaaccggc gagggccaag gcctcgccgg cgaggacgag
    14701 cgacccggcg acgagtatag gggcgtcctc ctcccctccg gcgaggaggg cgagcgcctc
    14761 ggcgagcgag gcggcgcgcg ggaggtcgtc ccgtccgaga cgtgccgcga gcgcgtccgg
    14821 ccggaccgcg cgaggcgaat cgggggcgac gaggacgacc tggcgggcgc gcgccgcgag
    14881 gggggcgaac atcccttcga ccgccttgtc gccgagggcg ccgaagacga gaccgacccg
    14941 tcccgcgagg cccacctcgt cgaggtacgc ggcgacggcc gcggcgcccg cggggttgtg
    15001 cgctccgtcg acgaggagcg gccgtgcccc cggccgcggg accgtctgga gccggcccgg
    15061 ccagcgggtc gcggcgacgc cgcgccggac ggtcgcgtca tccgccggga cgagcgcgcg
    15121 cgccgccgcg acgccgaggg cgacgttcgc gcgctggtgc gcgccggcga gcggcgagac
    15181 gtcgtcccac gcggccgacg gcatcacctc cacgaggtcc gcgccgaccc gcgccgcctc
    15241 ggcgtggagg accccgagca tcccgccccg ctccgtcgtg agggcgaggt gtccggggcg
    15301 gaagacaccc gccttctcgc gcgcgacgtc cgcgagcgtc ggcccgagcg tctcgaggtg
    15361 gtccgcgtcc acgttcgtga cgacggagac ctccggctcg aggacgttcg tcgcgtcgag
    15421 ccggccgccg atccccacct ccacgacggc gacctcgacc cccgcgcgcc ggaagagctc
    15481 gcacgcggcg acgacgagcg tctcgaagta ggtcgggcgg agcggcccct ctcccgagac
    15541 ggcggcgacg agggagaggg tcgcgtcgag cacggcgtcg gagacgtcgc gcccgccggg
    15601 gaggcggacc cgttcgttca cgcggacgag gtgcggggag gtcgtcaggc cgacacggag
    15661 acccgccgcc tcgaggacgg ccgcggccgt ggccgccgtg gagcccttcc cgttcgtccc
    15721 ggcgacgagg agcgttcgga acgagtcctg cgggcgtccg agcgcggaga ggagggcccg
    15781 ggtccgggcg aggccgggcc gaatcgtctg cggaccgaga gcctcgagcc agcggagacc
    15841 ggcgccgggg atcacgccgc gaggagacgg aggacgttcg cgagcgtcgc cttcagctcc
    15901 ttgcgcggga cgacgacgtc gacgaagccg tggtcgagga ggaactcgga gcgctggaag
    15961 ccctccggaa gggtctgccg gatcgtctgc tcgatgacgc gcggtcccgc gaacccgatg
    16021 agcgccctgg gctcggcgag ggtgacgtcg ccgagcatcg cgaaggaggc cgtgacgccg
    16081 ccggtcgtcg ggtcggtcag gaccgtgacg taggggatgc gcgcctccga gagccgcgcg
    16141 agcgccgcgg agaccttcgc catctgcatg agggagagga tcccctcctg catccgggcg
    16201 ccccccgagg cggtgacgac gacgaaaggg accttctcgg ctgcggcctt ctcggcggcg
    16261 cgggtgagga cctcgcccac gacggagccc atcgagcccc ccatgaaggc gtagtcgagg
    16321 gcggcgacga gcaccctcgt cccctcgatc gtcccctcgg cggccgcgat cgcgtcgggc
    16381 cggccggtcc gtccgccgta ctcccgcagc cggtcgcgat agcgcttcga gtctcggaag
    16441 cggagcgggt cggtcgggtg gacgtccccg aagagaagct ggaacgcccc gtcgtcgaag
    16501 aggagcttca ggcgctcgtc gaccgagagg cggaagtgga agccgcactt cgggcagacc
    16561 cgctggttgc gctcgacctc cttggcgtag agggtctcgc ggcagccgtc gcacttgagg
    16621 aagtgcccct cgccgagcga ggtcgccgtg cgcatgtcgc gcagggcgcg gggcgccttg
    16681 tctttccgga accagagggc catgggggcg ggatcttacc ccctccctcc gtcccggacc
    16741 gccgggggcg ctccgagggt gaccagcggc gtcccgtcgg ccagcggcgt gacgcacgcg
    16801 gcgactccga agagctcgct gatcgacctc tccgtcacgc tctggcggac cgggccggcc
    16861 ccgaggacgg ctccgcgggc gaggaggacc gcgtcgtcgg cggtccgcgc cgcgaggtcg
    16921 acctcgtggg tggagaagac gcagcagacg ccgcgggtcg cgagacgccg gaggacgtcg
    16981 aggacgagga agcggtggcg ggggtcgagg ctcgccgtcg gctcgtcgag gaggaggacc
    17041 cgaggctcgc ccgcgagcgc ccgcgcgaga tagacccgct gccgctcgcc cccggagagg
    17101 ctcctcacgt cccgctcctc gagtcccgcg gcgtcgacct cttcgagcgc ggcgcgggcg
    17161 atcgcgacgt cgcggcgcga cggggtcgag aaggcgccga ggaacggcgt ccgccccagg
    17221 aggacgacgt cgagcgccgt cgccgggaag aagggctcga agccctgggg aaggtagccc
    17281 agctgccgcg ccgcctccct ccgcggcacc tcggagatcg gccggccgcg cagcaggacc
    17341 gtcccgcgcg aggggggaag gagcccggcg aggaccttca ggagcgtcga cttccccgag
    17401 ccgttctcgc cgagaagggc cgcgacgcgc ccctcggcga acgtcaggct cacgtcgcgg
    17461 aggacgtcct tcccgcgcgc gtatccggcg aagacggcgc gaagctcgag aggcgtcacg
    17521 cccgcctccg gaggaggacg aggaaggcgg gcgcgccgag gagcgccgtc accgcgccga
    17581 tcgagatctc gttcggggag aagagcgtcc gggcgaggag gtcggagagg acgaggagga
    17641 cgcccccggc gaggaaggag cccgcgagga gggcgcggtg gccgtacgtg agacggcgga
    17701 cgagatgcgg gaccaggagg ccgacgaacc cgatgatccc ggcgtgcgcc accgccgcgg
    17761 ccgcgaggag ggaggccgcg acatacgcga tccgcttcgc ccgctcgacg gagaccccga
    17821 gcgtgaacgc ggtctcctcg ccgagggtga gggcgtcgaa ggcgcgagcg agcgggagga
    17881 ggacgacgag accgaccgcg gagtagaccg cgagcgaaac gacggcgtcc ccggtcgcgc
    17941 ccgcgaggga gccgagcatc cagaagacga agccgcgcgt gtcggaggcg tcgccgaccg
    18001 agaggaggaa gaggaggacg gccgaggcga gcgcgttcac gacgacgccc gacaggagga
    18061 ggcggccccg gtcgagccgg cctccgacgg aggcgccgag gccgacgagg aggacggcgc
    18121 cgagcgcgcc cgcgagagag gcgagaggaa gggcgaagga gccgaagagc ccggcggtgc
    18181 cgaggccgag gacgacggcg gcgagagtgc cgcaggccgc tccccccgag acgccgagca
    18241 ggtaggggtc ggcgagcggg ttgccgagga gcgcctggag cgcggcgccc gagagcgcga
    18301 gggccccgcc gacgaggagc cccaggaggg cccgcgggag gcgcaggtct cggacgatcg
    18361 tccgcgtcgc ctcatcgccg tccccggcca gggcggcgag ggcgtcggtc ggcgcgatcg
    18421 gaacgctgcc gacgaggagc gacgcgacga ccgcgccgag ggcgaggagg gcgaggagcg
    18481 cgaggaagcg acccgggctc ctcaaggcgt ccccgcgtcg agcttcgcga cgagcgtctc
    18541 gagggcgtcg acgaggcgtg gaccgggccg ctcgagccag tcgccgtcga ggacgacgac
    18601 gcgacccgcg cggaccgccg ggaggagctg ccagcggctg gccggtgaga ggacggcctc
    18661 gaacgcggcc cggttctccc gcgtgtcggg ccggacgagg aggctcgggt tccaccctac
    18721 gagcgtctcg tgggagacgc ggggccattc gccggcgccc ttcggcacga cgttctcgag
    18781 cccggcgcgg tcgaggaggt cgccgacgaa cgtgtcgcgg cccgcgacga tgggcgggtc
    18841 gggccagatc accgcgaggg ccgtcggccg gcgggtggcg cggcgggcgc gaacgcgctc
    18901 ctcggccgcc gcgacgcgcc gctgcatccg ggcgacgatc cgctcggcct ccacgtcccg
    18961 gccgagggcg gcgccgacgc gccggacgtc ggtgtagacg ccgtcgaggg aggtcgcgtc
    19021 ggtgacgacg acgcggatgc cgagcgcggc gagccgctcg gcggcccgcc tgtcggtccc
    19081 gtcgcgcgag acgaccgcga gatcgggcgc gagcgccgcg atccgctcga ggtcgggaac
    19141 gaagccgccg agccgcgcct tcccctccgc accgggcaga cccgctgcga agtcggagac
    19201 gccgacgacc cggtccgccg ccccgagagc gaagacgatc tccgccgcgg acggggccag
    19261 aacgacgatg cggcgaggcg gcgccgccgc gaggggcgtc gcgacggcga cccacgcgac
    19321 ggcggccgcg gcgaggagga tctgacgcgt gcgcaaggtc gaggaagaag gcgaaggccc
    19381 cggcgccctc acccgaggga gtcgccgggg ccggtccgcg gccgatcgga cggcgggcgc
    19441 ccgccgcccg tcaggactcc tcggactcct cgaggtcgtt ctggatcccg aggcggaggc
    19501 acgcggcggc gtacatctgc cgcgcggcct tctggccgtt ctgaagctcg gcgaggcgct
    19561 ccttcacctt ggccagctct tcctggatgt tggcctccag gtcgctgatc tccttcttcg
    19621 tgacgcggag cgtgttctcg tagaacttcc tctggtcgtc gctgaggctc atgccggaac
    19681 tccttctcga gctacttcac cgtgatattg aacgtcttcc acatcttcac gcggacgccg
    19741 gccttcgtgg cgggctcgat ctgagccccc ttgacggccg agacggccgc atcgtctgcg
    19801 accgggctac cggacgactt gacgacgcgc gtcgaggtga cgcgtccgtt ctcgtccacg
    19861 agagccatga ctgcggccgg ccccgtgacg cgccgcgcct gggcggggag ccgcgggaac
    19921 gccccgagct tgacgagccg gggctcccgg acgcccggac cctggccgac gaggtcgccc
    19981 tcgcgcacgg cggcggccgg tgcggcggcg gccggcgccg gagtcgccgg acgcggcgcc
    20041 tcgacggcgc gcggaggatc ggtgggacgg accgggaccg gcgtcggctc gggcgcccgc
    20101 gtcggcgccg ggaccggggt cgccgtcgcc gcgggcttcg gcggctggag cggctggctc
    20161 ggcgcgagcg agacggccgg agcggccgtc gccggggcgc cggtggccgg cctcggctgc
    20221 tgctcggcgg ccgccttctg agcggcttcg cggagcttct tcgcttcctc ggccgtcgcc
    20281 ttcttcagct cggcctcgag cgccttggcg tcgatcacct tctcgggagc ggtcggcgcc
    20341 tcggtcgtgg ccggggcagg ctcggccacc gcggtcgggg tggcgacggg aggcggagcc
    20401 ggctcctcct tctttccgcg cgtcaggaag acggccgccg cgatcaggac gacggcggcg
    20461 acgccgatcc cggcgaacag ccccgtgcga ctcttccccg cgtcctcgtc gacgctgatg
    20521 gccggctggg agacctcgac gggctccggg acggggaccg gctcggggac tttctccttc
    20581 ttggagatga cgagcccctt ccccttcttc ttctcttccg gagcgggggc ctgcgcggcc
    20641 gcctgcgcga acgccgcgtc gatctgcgcg tcggaggcga cgggggtcgc ttccacgagg
    20701 cggtggagga agatcgcgag gtcggccggc cccggagcgg gctggaagga gtagaggacc
    20761 gagttgatgt cccgctccat ctccgaggcg ttctggtagc ggtcctgcgg gttcttcgcg
    20821 agcgccttga ggacgatctg gtcgaccttc ggcggcacct cgccgttctt cgccgagggg
    20881 ggcgtcgagc gcgcgtcgcg gacctgctcg aggatcgtca ggtcggtgtc gccgctgaag
    20941 agccgctcgc ccgtcagcat ctcgtagagg acgacgccga gcgagaagat gtcggtgcgg
    21001 cggtcgacct tcttccccca cgcctgctcg ggggacatgt actggagctt gcccttgagg
    21061 gcgcccgcct gcgtctgctg gaccttcgtc gccgctttcg cgatgccgaa gtcgcagagc
    21121 ttgatgtcgc cctcgaagct gatgaggacg ttctgcgggg agacgtcgcg gtggacgagg
    21181 ttcagctcct tgccgtccat ccccatccgg cggtgggcgt agtcgagggc gttggcgacc
    21241 ttcgaggcga cgaagagcgc gagctcgacc gggagcgggt accccttctc cgagccctgc
    21301 ttcaggatgg agcggaggtc cttgccctcg acgtactcca tcgcgatgta gtggtaggcg
    21361 tcttccttcc ccaggtcgta gatgtggatg atgttgttgt gggtcagctg tgctgcgagc
    21421 ttggcctcgt cgacgaacat cgtgatgaag tcatcgttgt cggccatgtg cgggaggatc
    21481 cgcttgatcg cgacgatctt ctcgaacccc tcctcgccgc gcatgcgggc cttgtagacc
    21541 tcggccatgc cgccggcggc gatcttctcg aggagctgat aacgtccgaa gctgccgggt
    21601 ccgccggccg ccggtttcgc cttcggcgcg ggggcgggct tcggcgctgg ggccggcgcc
    21661 ggcttcgggg ccggggcggg aggggccacg ggaggcgcga cgggagcggg ggccggcgcg
    21721 ggcttctcgg ccggcttgac cggccgaagg ccggagagcg tctcggtgag catccggtcg
    21781 acggcggtgt caatcttcgg cttcggggcc gggatcggct gcgacggcgg gttcgcctcg
    21841 acgcgcggcg ccgggacggt cgcgggcgac ggcttcgcgg aagctgccgg ggccggcttg
    21901 gcgccggggc gcgagaggat cccagaaaga gtctgctcga gcttcttgtt gatccccggg
    21961 tcggttccga ccggcgcccc ggggcgtgcc ttctcctcgg cctccacacc ggagatgacg
    22021 tccgagaaga tgtcgtcgga agagaggagc ggaggcgcgc cggctgcggc gaacggctcc
    22081 gcggacccgc cgccgagagc tgaggagatc tccccgagga gggcctcctc ggagaacggc
    22141 ttctccagga tcccgctggc gccctcccgg gccgcgtcgg ctctcttgtc cttccccttg
    22201 tatccggagg ccatcagcag gatcggaggc gtcgccccga gcgcctgcat cccccggatc
    22261 acgtcgcgcg tccggagctt cggcaggacc gtggagatca ggacgaggtc gggcttcgcc
    22321 cgcctgtagg cctcgagtcc gtcctcgccg tccttcgcga cgacgaggtc gaagtccttc
    22381 ccggcgagat agcccttgat ccggtcgagg tatctcggct cgtattcgat caggagaatc
    22441 gtcttcgcca tacgtcgtgg ggccttcggc ccggtccccc ccgcactccg aagtctcctc
    22501 cggaaagtca cttattatcg ccccggacgt gagcctgtca acgaagtttg ccgctcccga
    22561 gcgcttcggc aggcgcttcg gacgagccta cgccgtccac gttctcggct gcaaggtcag
    22621 tcagatcgag gccgacggga tcgcccggac gctcgagtcc gaggggttcg agccggcgcg
    22681 accgggcgag gcggcggagg tcgtcgtcct ccacgcctgc accgtgaccg cccgtgcgga
    22741 ccgcgacgcc ctccgactgg cccgtcggct gcggcgcgag aacccggcgg ccctcctcgc
    22801 cgtgacggga tgcctcgccg agcgggaccc ggacggcctc tcattgcgtc cggagatcga
    22861 cgtcgtcgcc ggccacggca gccggctggc cctcccccgc cttctcgagg acgccgccgc
    22921 gggcctcctt cccgggaagg tcgcggagcg gacggcctct ccggccgagg tcctcgcgtc
    22981 gcccctcgcc gccggaggga cgggcaggtc ccggacgttc ctcaagatcc aggacggctg
    23041 cggcaggcgc tgctctttct gcatcgtccc ctcgctccgc ggcgccgaga ggagcgcgcc
    23101 ggcgagcgag gtcgaggacg cgatccgggc cctcggcgag gacggcgtcc ccgaggtcgt
    23161 cctctgcggc gtccatctcg ccgccttcgg tcacgaccac ggcgagagcc tcgtcggcct
    23221 cctccgccga ctcgaggacc gcccacccgc ctgccggctt cgcctctcct cgctcgagcc
    23281 gatggaggcg ggagaagccc tcgtcgacct cgtcgcctcc tcccgcgtgg tcgcgccgca
    23341 cctccatctg ccactccagt cgggctccga cgccgtcctt cgacgcatgc gccgggggat
    23401 gacccgcgca cgcttccgcg acctcctgct gcgcgccgcc cgcgcgaacc cgcggctcca
    23461 cctcgcgacg gacgtcatcg ccggcttccc cggagagacg gacgcggagt tcgccgagac
    23521 cgaggcgctc ctcgcggagc ttcccctcgc gtcgttgcac gtcttcccgt tctcgccccg
    23581 gtccggcacg gccgccgccg cgcagccggc cggcgcctcc gtcccggcga gcctccgtac
    23641 ccgccgcgcg gcccgcctgc gcgcgcgtgc cgccactctg ctccggcgtt tcgccgccgc
    23701 ccacgacggc cgcgccgccg acgtcgtcgc gctgcgggga gacgtcgggc tgacggagac
    23761 ctacgtcgaa gtcgcgctcc caccgagcgg gccgcccccc gcgagccggt tcgccgcgct
    23821 cctcgcgctc cgaccggacg gcggcctcga cgcccgcccg gccgtaccgt tctcctgcta
    23881 gactccgccg ttcctgaatc ctggcccgag cggcccgcac tcctcgaggt gagccatgtc
    23941 cggacacagc aagtggagca cgatcaagca caagaagggc gccgccgacg ccaagcgcgg
    24001 ccggctcttc acgaagctga tcaaggaaat cacgatgtcc gccaaggtcg gcggaggcgt
    24061 cgtcgagtcg aacccgcgcc tgcgcagcgc ggtgctcgcc gcgaagaagg cgtcgatgcc
    24121 ctccgacaac atcgacaagg cgatcaagcg ggggacgggc gagctcgaag gggtcaccta
    24181 cgaggaagtc gtgtacgaag gctacgggcc cggcggcgcg gcgatcctca tcgaggcgat
    24241 gaccgacaac aagaaccgga cgacggccga gatccgccac ctcctctcga aatacggcgg
    24301 gaacctcgga gcgacgggat cggtcgcgtt ccagttctcg aagaagggct acatcgccgt
    24361 cgagaagagc tccgccgccg aggacgcgct catggaagcg gcgctcgaag ccggcgccga
    24421 ggacttccag gccgaggatt ccgacgtcta cgagatctac accgacctcg cttcgttcga
    24481 ggcggtgaag gcgcagctcg aggcgaagaa gttcgccctg cagaccgccg agctgcagat
    24541 gatccccgcg acgatggtcg ccgtcggaga caaggcgccc gcgctcctca agctcctcga
    24601 catgctcgag gacaacgacg acgtccagaa ggtctggtcg agcggcgaca tcgacgacca
    24661 gtacctccag gacgcgtagg cccaccgggg ggcgctgctc gccgcccccc ggccccccga
    24721 cgacgaggcg atggacggac ggtcgattcc ctctcacgcc ctgagtcccg aacggcggtg
    24781 aggatcctcg gcgtcgaccc ggggagccgc gccgccggct gggcgatcgt ctccttcgac
    24841 ggcgcgccgc ggctcacggc cgcgggcgtc ctccggccgg cgacgagctc cagcttcgcc
    24901 gagcgcctcc tcgcgcttca cgacgccctc ctgacggtca tcgagcggga acgcccggac
    24961 gaggccgccg tggagcgggt cttctcgggc gtcaaccctc agagcctgat cacgctcggc
    25021 gaggcgcgcg gcgtcctcct cctggcgctc gcccggtccg gcctgcccgt tcacgaggtg
    25081 acccctgcgg agatcaagaa gaccgtgacg ggcaccggcc aggccggcaa ggaacaggtg
    25141 cgccggatgg tcctcgcgct cgtcggcgcg ccggccggct cctcccggct cgcgttcgac
    25201 gcggccgacg ccgccgccgc ggccctgacg cacggattcc ggacctccgc cggccgccgc
    25261 ggcctccgct ccgggccgtc ctcctccccc gtccggccgc gcgtcgccgg ttgagcatcc
    25321 ccgggggggg tgctatcgtc cggtctcgcg agaacgcgca cgcatgtctt tccaaggctc
    25381 cctcaaagaa cttccgctcg cggacatcgt tcagctggtc gccgtttctg ggaagaccgg
    25441 gatgttctcc ctgacgcgcg ccgccgagag gggcgccgtc tacatccaga acggccagat
    25501 cacccacgcc aaggtcaacg acgtcgaggg tgaggacgcc atctacgcgc tcgcgctctg
    25561 gaacgaggga cagttccagt tctccgccgg cgtggagagc gagacgcgga cgatcacccg
    25621 ctccaacacg aacctcctga tggaggccgc gcgccggagc gacgagtgga agatcctctc
    25681 gaagaagatc cccacgaccg aggccgtgcc gcagctcgtc tgccgcgaag gcctgagcga
    25741 gcccgtcacg ctgaccccga acgagtggct cgtcgtgacc gggaccgacg gggtccggac
    25801 gatcgaggag atcgcgcgcg ggacccgcct ctccgccttc gacgtcgcca agacgctcta
    25861 cggactgatc acggccgagc tcgtcgagat ccgcacgaag gccgaggccc ggccggcccc
    25921 acccgccacc gctcccgccg cgaccgcgcc ggcgcaggcc tcggggcccg tcgtcacgac
    25981 gcacagcggc gtcttcaacg ccgtcacgcg cggcgacgag cgccgcagcc tccagatcct
    26041 ctgcacgaaa gtgaagcagg aagcggagag cgccgccctc gccccgggcg atcccgcgat
    26101 cgatcgcctc taccgtgccg ctctcggcga ggtggacaag ggccgcggcc tggacgtcgt
    26161 ccgcgagctg atccggagcc tggagcagac ggtcgccgcg ctgcgcggac gagaggccag
    26221 cctcgcgttc cgcgagaaaa tggccccgct gctgtagtgt tctcgggaaa cccgcgcccg
    26281 ggtttcccga acccttccgc ggcacggagc accggagctc ccctttcgcc atttcgatcc
    26341 tgctgtagcg ggaaacccgc gcccgggttt cccgaaccct tccgcggcac ggagcaccgg
    26401 agctcccctt tcgccatttc gatcctgctg tagcgggaaa cccgcgcccg ggtttcccga
    26461 acccttccgc ggcacggagg cgccgctctc tatactcgtc tcgcttgagg cgtgctttcg
    26521 tcctcctcgc tctcgcggcg gttccgcttc tggcggagga gccggcgcgc gaggagcccg
    26581 cggggatcat tccgcggatc gcggtctacg tcccggaggg cgaggcggag atccggctct
    26641 cgaagctcgt ccggagctcg ctcttcgaga cgcagttcga gtacgacttc gtctcgggcg
    26701 acatcgcggc gttcctccgc tacaagtact acggctcccg ccagagcttc acgctcgcgg
    26761 cgttcgaccg ggtctccttc gcggcgctcg agtcgctctc cctcgggtac gaccgggtcc
    26821 gcggcctttc ggcgttcgtg cggcgtcccg tggggctcta cgggcgcctg gtcttcctgg
    26881 cggaggtcga ccggttcaac gtctccggac cgaagaagcc gcacgagtcc gccgatgacg
    26941 gccagacgaa cctcttcggg aaggtcggct tccagtgggg gaccgaggac gacgacttcg
    27001 ggaaccggat ctccggcgac ccgaacgacc gggtccggaa cctcttcacg gtggcccgcg
    27061 cgttcgcccc ggggggccga ggcctctcac tcggggtcac ctacggcgta ccgatcctgg
    27121 actacgacta cctgaaggtc gagggggagg cgatccaggc gatcgacgtc gggaccagcc
    27181 gcctcgtggc ccggctccac gccggcttct tccctcacaa gccgccgagc gagggcgagc
    27241 cgccccccgg gggccttccg ttccggatcc cggccggcga gtacctctcg ctcggggggc
    27301 gcgacgcgct ccgggggctg cgggatgcgc cgttcgggac ggaccagctc cacgtcacgc
    27361 tcgaggcctt cgtcccggtc ttcgtcaacc gcaactcgga gttcctgaag gccacgtgga
    27421 acaccctgta cgcggtcggc tacgtcggga cggggaacgc cggagtcggg cgcgacgttt
    27481 acacccgtct cggcgactac cggcaggacg ccggcctcgg attcgaggtg gccttccgga
    27541 tctcgcgcta ccgggtcttc gcctccgccc tggcggcccg cgagatcggg gggggcgggt
    27601 ctccccgttt cctgatgacg tttcggacgg tcaattgact ttgccgggaa atctcgtgct
    27661 gctagactct tgacaactga tccgagccca ggacgcggag cggcttcccc ttccccggta
    27721 cgggggtgac tcgtccggtc cccggctgga aataaggaga ctcgatgcga cggatgcgga
    27781 ccggactcct cctcgcgctg gccgtggctc ttctcgcggc gcttccgctg agcgctcagg
    27841 tggctcccac cctcaccggc gagaccgggc tcttcggcat ccaggacgcg gacacggttc
    27901 cccagggcaa gttcgcgttc tcgctcttct acagcatgta cgaccggacg gccgcgtccg
    27961 tgccgctgct ggcgcctctc ggggacgacc cgctccgata cagcaccgac aagctcggcc
    28021 tctccatcac gttcggcctc ctgccgaact gggaggcgtc gttcacggcc gggcagcggt
    28081 actactcctc ggacgaccgg ctctgggccg ggaacatcgg ggggcggaac cgcgtcggcg
    28141 aggtcgacca cgacgagacc gacaagctcc ggatcgggac gaagtacgtc ctcaaccgga
    28201 aggacccgct gaaggtcgcc atcttcgggg ccgcctacat tccgacccag cgcaagagcg
    28261 acgacgccgc gatgtcgtcc tatcgcgccg actgggagtg gggcttctcc gggacctacg
    28321 gcatcttcac ggggcagctc gcctacctct ggacggccga ccacgacgac cagtacgacg
    28381 agacgtacga gatcgcgaac gagctgtcgt tctccgccgg cgtcggcgtc ccgctcatcc
    28441 ccgaccgcct gaaggggatt ttcgagatcc accgcgtcat gtacgacggc gggacgaccg
    28501 agccgcccga cctcaccgac gccgtcctcg gcgcgcgctt cgcgctcggc aagagcggcc
    28561 tcgtggccgg cgccgggatc cgcgcgaaca tcgaccggtg ggtgaagtac ggcagctcgc
    28621 cgagcaacat cggcggcctc ctgcagctct cctacgcccc acagcccgag aaggtcgtcg
    28681 aagcgccgaa ggtcgtcccc tcgccgcacg tcccgcctcc ggtcgagccc acgcccgccc
    28741 cggcccccgt cctcgagccg gcgcccgcgc ccgtcgtgga ggccccgccg gcgccgaagc
    28801 ccgagacctc gacgaccgac gagatcctct tcgacgcggc gaagagccgc ctgacgaaca
    28861 tcgcgaaggc gatcctggac ggtgtggcgc tccgcctgaa gaacaacctc tcggcgacct
    28921 gcgcgatcac gggccacacc gacccgggcg agaaaggcga ccacgccgcg ctcgcgaagg
    28981 cccgggccga tgccgcgaag gactacctcg tcaagcggca cggcatcgac gcgtcccgga
    29041 tccaggtcga cggccgggga gacgcggagg ccgcggacga cgcgacgcgc aaccgtcgcg
    29101 ccgtcgtcac ggtgacgttc ccctagccgg cgcccttccc cggcagagac ctcgagcggc
    29161 cggccttcgc cggccgctct cgtctctccc cgacgcgcta aactcgcgcc gcttggacag
    29221 cagcggcgcc ttcacgaccg gaacgagccc cgctctcggc ggggtttttc gttcgcgcgc
    29281 gctcgtcgtg ctcgcgacgg cggccgtcgt cggcctgctc tgggccggga agatcctgtt
    29341 cgtcacgatc ttcttcgccc tcttcctctc gttcgcgctc cacccgttcg tggagctcct
    29401 cgagaagctg cgcgtcccgc gcggcctcgc ggcattcgtc gtcctcctcc tcctctgcgc
    29461 gctcttcgcg ttcttcatcg tcaacgcgct cgaccaggcc caggccttct cgcgcgactg
    29521 gccgtcctac gagcggaaga tccaggcgct cacggcgcag gcgcgcggcg tcttctcgcg
    29581 catcgaggag gggacgcgcc ggctcctctc cgacggcggc gcctcgggag tccgctccgt
    29641 ccgcctcgaa gaggggatgc tcgactcgat ctcgcgcgtc gtcctccagg cgaagacgat
    29701 cttctcgctc ctcctctaca ccgcggcggt cccgatgctc gcgttcttca tgctgaagga
    29761 ccgcgagaag tactccgggg ctctgacgcg catcctccgg aggaaagggg ggcgcgccgc
    29821 ctccgacttc gccgtcaacg tcgcgcacgt cctctccggc tacgtcctcg gcgagctctt
    29881 cgtcgtcctg atcaccgcgt gcatcacgac ggcgggtctc ctcatcctga aggtcccgta
    29941 cagctacgtc ctcgggccgc tcgccgggct ctgcgtcctg ctgccctacg tcggcgtcat
    30001 cctctcgacg acgcccgcgg tcttcgtggc gttcctcggc accgacgagc cgttcctcgc
    30061 cctgaaggtc gtcgcgttct actcggtcgt gcagttcctg gaggggaacg tcctgacgcc
    30121 gttcatcgtc gggagccggg tccggctcca cccgctcgct gtcctcgtcg cgttcctctt
    30181 ctgggggatc ctctggggcc tccccggggc gatcctcgcc gttccgctca ccgcggcggt
    30241 gaaggtcgtc ggggagcggt tcgagcgctt cgcgccgtgg gcggccctcc tcggagaccg
    30301 gacgcccgac ccggagccgg agcccgacga gccgaaaccg gagccggaag cggccgggtg
    30361 agcccgtgtg cggccgctac gtccagacga gcccggccga cgccctcgcc gcgcgcttcg
    30421 gcgcccgcct gccggaaggg ctcgcgtggg tcccgcggtg gaacgtcgcg ccatccacgc
    30481 cggcgcccgt ggtccgtgcc gacgggcccg gacgtgagct cgccctcctg acctggggcc
    30541 tcctcccctc gtgggcgaag gacgccgccg gcctccggcc gatcaacgcc cgcgccgagg
    30601 ggctcgccgg gaagccgatg ttccgcgacg cgctccggcg acggcgcgcg ctcgtccccg
    30661 ccgacggctt ctacgaatgg caggcctccg ggccggggaa ggtcccctgg cacttccggc
    30721 tgaagggcgg aggggcgttc gcgttcgccg gcctctggga gcgccgcgag gctccgggag
    30781 gaccgccgct cgagacgttc gcgatcgtga cgacggaagc gaacgacgtc gtccggcccg
    30841 tccacgcgag gatgccggtg atcctcgacg ccgaggccga ggcgctctgg atgcgcgagg
    30901 gcgcgctctc tcccgacgag gccgcgcgct gcctccgccc gctcgacgcc tcgaggatgg
    30961 aggcgtgggc cgtctcctcc tgggtgagct ctcccgcccg cgacgggcca cgctgcgtcg
    31021 agccggcggg ctgacgcggg ccgggcgcgc gccccgcgcg acctcccttc ccttccgcgg
    31081 ggcggctcca tagactgacc gcgccatgca ccccgagcac cgcgcggcct tcaaccgcgc
    31141 cttcacgtcc gcgctcgccc gcggccaggc ggaggacctc gcgcgccgcg ccgggcgggc
    31201 gcccgggttc cgcctcgccg agacgccggt cttcctgacc cgcgagctcg aggagcgcct
    31261 cgtctcggcg tcgcacggga tcctcgcgca gctctcccgc cccgagaccg ttgcgcggct
    31321 gcggcgcttc gtcccgccgg agtgggacgc gcccggaatc gacgagctcc cgtcgatcgc
    31381 gcagatcgac ctcgccgtcg cgcgcgctgc cgacgggagc ctcgagccga agctcgtcga
    31441 gctccaggga ttcccgtcgc tgtcggcttt cgaggtgctg caggccgacg catgggaagc
    31501 ggccctctcg gagattccgg gcctgcccgc cgggggatgg agcccgtact tctccggcct
    31561 gacgcggacc acttacctcg acctccttcg tcgcaccgtc gtggccgggc acgacccgga
    31621 gaacgtcgtc ctcctcgacc ttcaccccga gggccagaag acggcggtcg acttcgacgc
    31681 gacgcggcgc ctgctcggtg tcgaggccgt ctgcgcgacg gaggtcgaga aggagggacg
    31741 ccgcctctac cgcaggagag gcggccggcg cgtcccgatc gagcggatct acaaccgggt
    31801 cgtcgtcgac gagctggtcc ggaccggcgc gcgcctcccg ttcgacttcc gcgacgacct
    31861 cgccgtcgag tgggcgccgc acccgaactg gttctgggcc ttctcgaagg ccgcgctgcc
    31921 gctcctcgac cacccggccg cgccgcggac gctcacgctg gacacgctcg acgcccttcc
    31981 cccggacctc gcgaagcgct gggtcctcaa gcctctcttc tccttcgctg gcgggggcgt
    32041 aaacgtggag cccaccgagg cggacgtcgc ggcggtcccc gccgccgagc gtgaccggtg
    32101 gtgcctgcag gagaagatcg agtacgcccc ggcgctgatc acggtcgacg gcggaggggt
    32161 gaaggtcgag atccggatga tgttcttccg gcccggctcc cggcgcgagt tcaccctcgg
    32221 cctgaacctc tgccggctct cccgcgggaa gatgcacggc gtcgacttca accgcgactt
    32281 cacctgggtc ggctcctcgg tggcgcttcg cccggcggcc gggtggcccg gggagagctc
    32341 ccgcggcgct tgacaggagc ggcccgcgcg gagatcgtct agaaaatgcc tcgggaggct
    32401 cacgtgccgc agaacctgaa gggtctgaag gtcctcttcc tcgtcgctcc gaagggtttc
    32461 caggtcgagg agctcgacaa gccgcgccgc gtcctcgagg cggccggtgc ggaggtcgtg
    32521 gtcgcgacga cggcggccgg cggcgcgacc acgcaggacg ggacctcggt cgacgccgtg
    32581 ccgatcgtcg agctgcggcc gaccgacttt gccggggcgg tcgtcgtcgg cggccccggc
    32641 tctccggaga tctgggagaa cgggatcgcg cacaagttct tccggatgac ggcgcacgac
    32701 ggcaagccgg tcggagggct ctctctcggc gtcggcctcc tcgcgaaagc cggcctcctc
    32761 gaggggaagt cggcgacgat ctgggtcacc ccggaggccc tgcgcacgct gaaggacgcc
    32821 ggggcgcgct acgagaagaa gcccgtcgcg gtggcgggcg gcgtcgtgac cgccgacggc
    32881 ccgtcctcgg cggagagctt cgggaacatc ttcgccgagc tcctggacgg ctcccggtcc
    32941 cgctccgggc gcgtcacgcg ctgacggcgc cgcgccgtcc cctcagatcc gcgcgagcgc
    33001 ggcgccccag tggaggcccg cgccgagggc cgtgaagccg acgagcgagc ccggcccgat
    33061 ccgccccgcc gcgaccgcct cgtcgtagac gagcgggagc gtggcggccg tcgtgttgcc
    33121 gtacttctgg atgttgttga agaccttctc gtcggggagg ccgaggacct tctgcaccga
    33181 ttcgttgatc cggaggttcg cctggtgcat caggaggagg tccagctcct cgagcgagcg
    33241 gccgcagccc gcgaggacct cccgaaccgc ttcgggcatc agctggctcg cgagccggaa
    33301 gacctggcgc ccctcgacga tcgggatcgt gccgagactc tcgaacatct ccggcgtgaa
    33361 gtacggccgc gacttgcagc cgccccccgg gacgtacagc ttgtcccagt ggttcccgtc
    33421 ggtcttcagg acgataccga ggaagccgcg gccgtccccc gactcgttcg cctcgaggac
    33481 catcgcgccg gcgccgtcgc cgaagaggac ggcccggtcg cgcatccccg tcgcccgctc
    33541 gtaccagtac gggtcgcagg gccccgggct cctccccagc atgacgtccc agaccgactc
    33601 cggccagggc atcaggccgg agtggacctc cgccccgacg aggaggacgc ggtcgaacgc
    33661 gccggagcgg atgaaggcgt cggccgtctg gaggcccgag aggaacccgg cgcactgcat
    33721 ccggatgtcg agcgccggcg tcgtcgtcag gccgagccgg gccgcgagga gaccaccgct
    33781 ccccgggaag tagtggtcgg gcgtcatcgt cgcgaagacg acgagaccga tgtccttcgg
    33841 cgcgaggccc gccttctcga gcgcccgccg ggcggcgtgc tcggccagct ccgccgggcc
    33901 gacgccgggc gcgccgaatc gccgctcgcg aatgccggtc cgcgggacga tccactcgtc
    33961 cgtcgtgtcc atgatccggg agatcatctc gttcgtgacg acctgaggag gcacgtagct
    34021 tccggaggag cggatgacgg tcgttctcgg cgcgttgctc atcgacgggg ccgaagtcta
    34081 gcggacggcc ccgccgggcc gaaggcgggc gacggtcgcg ccccagccgc cccgctccgg
    34141 gggcgcgtcg cgaaactcca cgacgagggg cgaccgggcc aggaccgcgc ggacgatccg
    34201 ccgctgcgtg ccgacccccc gtccgtggac gatccggacc tcgtcgaagc cgcgcacggc
    34261 ggcctccgcg aggtactcct cgacgacgga cgccgtctcc gccggacgga aggcgtgcag
    34321 gtcgatcgcg tcctccagcg ggaggaccac gggaacgtcc tcttccgtgt ccacggccac
    34381 agtgtagggc cggcccgccg gttgaaggtg cgcggccggc ggcggagaat ggccgccgaa
    34441 cggaggaatg ccatgaagca ccgattcgtc gtggccgcgg ggctcctcgc ggccctcgcc
    34501 tcgtccacgg cgctcgccgc caagccccgg gtcgccgtca tcgagttcaa ggacaagacc
    34561 cagcactatt cctggtaccg cgccggagag gccgcccagg acatgctcgt caccgagctc
    34621 gtcaagtccg ggaagtaccg cgtcatcgag cgcgagcagc tcgcggcgat catgcaggag
    34681 aagaacctct ccctctccgg cgagatcgac ccgaagacgg ccgtcaaggc gggcaagctc
    34741 ctcggcgtgg agtacatcgt caccggcgcc ctcaccgagc tcggagtcgc cgaccgcggg
    34801 gctcgcgtcc ccggcggcct cttcggaagg cttccgtccg tgaacgtcgg gtcgcagaag
    34861 atggacgccg cgatcgacgc ccggatgatc aacacgacga ccggcgagat cgtctgggcc
    34921 gactccgcga aggagtcgac gtccgatgcg aaggtcttcg tcgcgggcgc cggcggcggc
    34981 gtcgaggacc ggcggaagct cgaccgcgtc ctccgccccg tcgtcgtcaa gctggccgag
    35041 tcgatgggga aggtcggcgt gtcgacctcc ggcctcggcg gcgcgagcga cgcctcgggg
    35101 atcgccggga agatcgcgaa ggtcgaaggg ggctcggtct tcctgaacgt cggcgccgag
    35161 gccggcgtga aggaaggcga cacgttcgac gtctaccggg tcggcaacgt catcaaggac
    35221 ccggacaccg gcgaggtcct cggctccgac gagacgaagg tcggccgcgt gaagatcacg
    35281 aaggtgatgg gcgcccgcct ctcgaccgct tccatcgtga gcggctcggg cttcaagccg
    35341 ggcgacatga tcaagaactg acgccgcaga gacggcggga gaaggaatcg gcgggcctca
    35401 cggcccgccg atttcctttt ccctcgtctc ccgtgtctac cggcggccga ggagctcgca
    35461 ggcctcctcg atcccgtccc cgcacgcctc ccggaggagg gcgccggccc ggcggccgtc
    35521 ccggacctcg acggcgcgga gcgcccgggc gacccggacc gcccggttcc cgggggcgcg
    35581 ctcggcggcc gcggcgagct cctcgtcgag gtcgccccgg acgtcgccga acgcgaggcc
    35641 gaggatccag ttcgcgtcgc gtcccgggtt catccccttt cccttcatca cgcgccggac
    35701 gaaccggagg acccgacctc ccgccgcgcc cgactggtcg acccggaacg cctcgtgcag
    35761 ctgctgccag gccgtggcgt tctcgccgtt caggaacgcg ctcccggccc gcgtgtagag
    35821 gtcgatgaag cgggcctccg gctcgtcgct cgtggaggcg atctcgcgcg cgatgcctcc
    35881 cgcctccgcg agcgcctcgc gcgtgagggt ccccttcgag cgggtggtgt cgagggcgcg
    35941 ataagcctct tcgagccgcg tgtcgagcga ccactggcct ccgtcgacga gcgcctcgcg
    36001 gcgcggcagg gacggatcag ccgaaacgac ggcgccggtg tcccgcgaac cggactccag
    36061 cccggagagc acgccgcccg tatcggagct cccccgctgc cccttgccga cgacgtcctc
    36121 ggaattcgtc gccgcgggac ccgggcgcgt ctcgacgacc tgacccatcg gaccgctctc
    36181 ctcgaccgac gacgccgcca gaggcgtgct cgcggcctgc ggcgccgtgc cggggcccgt
    36241 ctccgtcggc gctccgcttc ccgcgctgcc gaagctgtcg gtcgccggcc cggtcgggac
    36301 cgtctccgcc ggagcgggct cggtggcgga ggtcgccccg ggggcgaggc tcgcgagggc
    36361 cgccggcttc tcctccggcg cggtctgccc cgggccaaag cgggagtacg cgagccagcc
    36421 gcctcccccg aggacgacga gaccggcgac ggtcgccgcc acgaggagcg ccgggctctt
    36481 cccggctcgc gccggcgcga ccggcggcgg aggcggcatc gaagccgcgg ccgccatcgc
    36541 cgtgtcgggg ctggagattc cggccgacgg gacgacgccg gcgcccgccg tcgcgacggt
    36601 cgggaggtcc gccgcgacgc gcgcccctcc cgcctcgagg accgtcgggg cgccgtggat
    36661 cgtcggagcc cccggtgggg gcgggggcac cgcgcggacg ggtgcggaag cggcggccgc
    36721 ggcgccgagg gagaggccga agaccgcggc gagcgccgag acgaggtcgg tacagctctc
    36781 gtaacgccgc cacgcgtcct tggcgagcgc acgcgacagg acgtcgtcga tggcgggcgg
    36841 aaggccgccg acgaacgacg tgaccggctc gggcgggacg ctgacgtgct ggtggaggac
    36901 gccgagcggc gtgtcggcct tgaacggcac gcgccccgtc agcatctcga acgcgatgca
    36961 ggcgagggcg tactggtcgg cccgcgcgtc gagggccttc cccatcgcct gctccggcgc
    37021 catgtacgag ggcgtcccca tcacggtccc ggtggccgtc agccccgaga ccgagtcggc
    37081 gctgcgggcg aggccgaagt ctgcgagcag cgggcggccg gaggcgtcga ggaggacgtt
    37141 gcccggcttc acgtcacggt ggaggatccc cttggcgtgc gcgtgatcga gcgcggcggc
    37201 gatggccgcg agccacggca ccgcctgcgc gggcgctacc ggccccgtga tccgctcctt
    37261 gagggagcct cccgagacga ggggcatgac gaggtacgag acctccttcc cctcgagggg
    37321 cgccgtgccg aagtcgtaga cggcgaggat gttcgggtgc tcgagccccg ccacgaggcg
    37381 cgcctcgcgg aggaaccgct cggcgaacgt cgggtccgcc gcgatcgggt cgtgaatgac
    37441 cttcacggcg acggtgcgat gcagggtctg gtcctcggcg cgccagactt ccgacattcc
    37501 gccggcgccg gcgcgctcga ggaggcgata gcggcccagg agggttccag gtgtcacggc
    37561 catggcatcc tcgtttccag gggaaggata cgaccgccgg gaagcccccg aatgcgaagc
    37621 gcgtcacgga accgggcgat cccggctccg ccgccgggcg agtggcggtg ctacagctcc
    37681 tcggcgacgt cggccccggc gagcgccccc cagagggcga tcgagccggg acccgagcgg
    37741 gaggcgtcgt ccagcgcctc ctcggcgcgc gagaggaggc tcgccgtgtc gaaggcgtcc
    37801 tccgggtagg cggcgagccc gagcgtcagg gagaaccgga gccccgtcgc gtcgaggcgc
    37861 gggtcccgga acgaggcgaa gttcttcgcg atcgcctcgg ccgctctctg cgcgatcggc
    37921 ttgtcggcgt cgaagatcag gatccagaag cggtcgccct tgccgcggcc gacgatgttc
    37981 accgaccggg tggcgcggac gagctgggcg gcgatcttcg ggaggagggc gtctccgtac
    38041 ggcatcccga agcgctgcga ggcctcgccg aggccgtcga tgcggacgtg cgcgacggag
    38101 agcggctggc cgcttcgctc ggcgcgcttg agctccgact cgaggcgctt gacgatcgct
    38161 ccctggttgt agaggccggt ggcgccgtcc cgctcggtct gctgctcgat gatcttcatg
    38221 agccgcgagt tgtggagcgc gaccgcggcg gtgtcggcga ggagcgaaag gcgtcccgcg
    38281 agctcggccg gccacggccc gcccggcgag cggaaggcga acgtgaggac gccgatctgc
    38341 tcgtcctgga agaggagcgg cgccgcgatg gcccggagcc cgggagatcc ggcggctgcc
    38401 ggatagaggg ccccgatctc ggcctcgtcg acgaggacga gcgccttttc ggcccacgcg
    38461 cggaccaccg ggtgcccggt gctctcggtc gggacgtggc gcggggcttc cgcggaaggg
    38521 tcgctcccgc cctgggcgag gactcggagc gacttcgtct gcgcgtcggc gtcccagaag
    38581 agggcgctct ccccggcgag gagcgcgatc gcgtgctcgc agagggcgtg cttgatgcgc
    38641 gggccgtcga aggtggtcgc gaaggagcgt ccgacttcgc gcaggcggtc gaggaagcgg
    38701 taggagtcgc gctcgcgctg gagcaggcgg gcggacgtga ccgcgtggga ggcctcggcg
    38761 gcgacggcct ggaaggcgcg caggagaggc ggatcgaaag cccacggccg gccggcgccg
    38821 acgccggaca gcacgccgag gaactctccg tgcgacacga ggggaacggc gacgaggccg
    38881 agggggcgcg ggagctcctt cgcggcgttc ggcatgggga cgagcgccgg cccgtcggcg
    38941 tcgagcgcga cgtcggagag gaggacggca gcctcggggt gggccagacg gtcgacgagc
    39001 gcgcgcggaa tcgtcccttc gaccccgggg acgctcgctc cgtctttcgt ccagacgctc
    39061 gacgggggcc tcccgccctc gaaggagcgg acctggacgg cgcagaggtc gaggccgagg
    39121 ccggcgcccg tctcctccac ggtcttcgcg aggatcgctt cgaggtcgtt caggccggag
    39181 agggccgcgt ggacccggtt gacgaaggtg acgtgctcgg ccgtccggcg ctccagctcg
    39241 atgaggcgtg cccgctcgag ggctcccgcg gcgaggcgcg cgagctcctg gacgaggcgc
    39301 atctcctgcg ccgtcaggac gaaggctccc cggcgcgaca tcgtcaggaa gccgaagacc
    39361 gtctccccct gctcgatcgg gatcgcgagg cggcgcttgc gtctcgggcc cttgctgtcg
    39421 tcggcgtcgt cctcgtcgaa cgcggggccg tgcgccgcga gcgcacggac gaggatcccg
    39481 tcgttgtcga tcgggacccg ctgccccggg acgaacccgc cgccggtgcc gggcgaggtc
    39541 ccctgcgcga ggacgctttc gacctctccc ttctcggggt ggtagcggaa ggcgcggacc
    39601 tgctcgaact ccagctgccg ctgcagcgag gccgcggtga gccggacgac ctcgtcggcg
    39661 ttcagggtgt ggagcgcgcc ggaggcgagc tcgaagagcg cgaccgcctc ctcgcggcgc
    39721 ttcacgaggg tggccacgct ccgctcgcgc tcgaggatcc ggccgaggat ccgggcgaag
    39781 gaacggatcc cgtcgtcgac ctccatcccc tttcccgccg actgcgcgag cgccacgccg
    39841 aacgcgccgc tgacctgctc gccggtcttc acggcggcgc agaggaagac gagccccgac
    39901 tcggcgaccc gcccttcgcg cccctccgag aggtgcgtct cgagggcgcg gccgaggcgc
    39961 tgctcggccg cggccgagac gatggaggag ccggcgcggc tcatcggggg ggccccgccg
    40021 gcgggaagga gcgcgacggc cacggcatcg aagaatcgga gcgccgtctc gaggacggcg
    40081 ccgatcgact cgtcgaggaa gcgcccctcg gcgaggacgg cgtcgaacgc cttcagcagg
    40141 gcgaggtcac gggcggtcgg cacggcggcg acgttctccg aagattgctc gggaccgaat
    40201 catagccggg aacgggcggc gcgcgatcgc cccctggctc agatcgccga cgcctcgccg
    40261 tccgtctcga cgcggcgcgc tccgagaagg ccctgggcca cgaggaagag catcccgacg
    40321 tcgcggaaga tcaccatcca catgtcccgg aagcgctcct cgcgggtcag gttcgcggcc
    40381 gtgacgtcga agcagccgca gtcgatcgcg ttcccgcgca ggaggttgac cgagatcgcc
    40441 acgatgaaga cgacgaggag ggcgccgacc agggcggccg ccgtcttccg ccagatcccg
    40501 gcgacgagcg cgaggcccat cagcagctcg gcccagggga ggacgagcgc catcacgttc
    40561 acgagcggtc ccgggagcat cttgtagttg tagaccatgt gcgcgaagcc cggcgggtcg
    40621 gcgatcttcg gcagcgccgc gacgatgaag aagaggccga gcgcgatctg cgtccggacc
    40681 gtcagccagg gatgggtgag ggcctcccgg agcgtcaccg ccccctcccc gtcgcgaccg
    40741 cgccgccgcg ggagagccag tccgggaacc cgtcccggta gacgaggagg ttctcgaagc
    40801 cggccccgaa cagagtctgc gcgagcatgt gggagtcctc gcagtcgccg cccgtgcagt
    40861 agaggacgac gggcagcgtc tggtcgcgac cctcggcgac gagctcctcg acccgctcct
    40921 tcaccaccgc cgcctcccag atcgggaagg agcgcgcgcc ggcaacgtgc ttctcgacga
    40981 agtccttcga gcgtcgggcg tcgaggaaga gggcgccgcg ggcgtggagc caggcggccg
    41041 cctcgccggt gatctcgacg cccggctggc cgtgcggcgg gaagcgggcg aggacctcgg
    41101 cgacgtggtc gggggcggct ggagacgacg gcgacgaagc ggccgggcgt tcctggggag
    41161 gcggcgcggg ctcccgatcc gcggccggac gccgtgaccc gggggcgggg gccggcgcgg
    41221 acggcgcggg acggtccggc accgccggag cggccgaggc gggcggcgcc ggctcgtccg
    41281 cggcgggcgc cgggtgcctt tcgaacggaa ccggcgccgg aggcttcggg acggagaagg
    41341 cggccgggta gtcgcccgga agctcgaggc gccgctcctt cgcggcgaac gcgttcgaga
    41401 cgagggcgca ggcgatcgcg aggacggcga gggcaccggc ctcggcgagg aagcggcgga
    41461 ggttcatctg gcggctccgt tccgggcccg aggcccgcgt cgcgattcta tgcaagggct
    41521 tcgcccttcc tctcgtgccg ttccggcgcg tgccgggcct cgccggtcgc tcgaatccgg
    41581 ccctcgcggg gctagaattg cgggtccagt ccagcgcggc aaggaagggg aagacgatga
    41641 gcgagaacac tctcacgatc cgggacaacc gcaccggcaa ggagtacgtc gtcccggtcg
    41701 agaacgacac gatcaaggcg atggacctcc ggaagatcaa ggtgaacccg gaggacttcg
    41761 gcctga
//
LOCUS       DAOONL010000025        38405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_818_length_38405_cov_21.774316, whole genome shotgun sequence.
ACCESSION   DAOONL010000025 DAOONL010000000
VERSION     DAOONL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 38405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38405
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_38405_cov_21.774316"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLP50_05995"
     CDS             complement(<1..439)
                     /locus_tag="PLP50_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HPA51139.1"
                     /translation="MSLESFVVRPKRRLVEKYREPVAWTELSNVDRQELAAEVAGLPS
                     GLASEEEEAKWFDLLMLHLQLSVLRHEPSFQRLSEQVRAICGLLEEKAAIPMVRERIV
                     LIQAMQSDEWWQDVTPTMLETARKHLRGLVSLIEKRRRAPVYTD"
     gene            878..3178
                     /gene="ccsA"
                     /locus_tag="PLP50_06000"
     CDS             878..3178
                     /gene="ccsA"
                     /locus_tag="PLP50_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPA51140.1"
                     /translation="MKKLVETLSSLKLAVGLLVVLLIGLSIGTILESRTDAATAQRVV
                     YYSWWFLALQGLFALNVACSLANLFPWGKKRAGFVTTHAALLLILAGATLTYFWKVEG
                     QLGLWEGESGATIENHDAQGNVVSRHDLPFSVKLVDFVLDTYPGTMRPAGFASEVVIT
                     DTDTGKSFPARIWMNHPLHHRGYTLFQSSYQQQGGREATVLSVSKDPGQLIVFAGYVA
                     VVLGMILVLVTRVSQARERDRERAALEAKVAEGGMSLPTMGKALLLALALLPLAAPAA
                     TPELEALRRLPVQHDGRTMPLDTLARETVWNVTGSHAFRGEDPAATFTGWLFDPRSAA
                     TTPVVKVGSKALAAAAGLPAGTKHVSFEQLVSNQRLMGLIRQAGMAAQREEPRRGVLK
                     DAEKLENRLNVLYGLLQRETVRPIPAAEGPKARWNRPEAVTKEALLALANGPRLAGWP
                     TAKKIDGEILYNRVNPAKLSWIVLLASLVVSIVAWSKASKALDWTSFALLAVGFGVMT
                     WGIGMRWAAGERIPAANMYESLLFLAWGVGLFAVIAYLVMRNRTVVLNAAAMSALTMA
                     LTDLLPMDRFIHPIAPVLAGTPWLAIHVPIIMVGYAVLALGVVVAHMQLAFAAFGKGK
                     GALVERLYELLYWYMHVGSILLLAGILTGSVWAASSWGRYWGWDPKEVWSLVAFLAYM
                     AILHLKVDKVIGTFGAAAISILAFQTILMTYLGVNYVLNVGMHSYGMGDSPIVKWMVL
                     VALVEVVFLAVCGVAYRDRRRMLAEA"
     gene            complement(3239..3589)
                     /locus_tag="PLP50_06005"
     CDS             complement(3239..3589)
                     /locus_tag="PLP50_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51141.1"
                     /translation="MMYRVTGAGSAVVSTLAPGTPHEMVSVWHRDGNDLVMTHYCAAQ
                     NQPRFRTKTVPANVIAMEFDGGTNLDSAKDVHIHAVKVELLGEDEIRETWIGWKGGKT
                     EEPPLVLHLARKKG"
     gene            complement(3861..5162)
                     /locus_tag="PLP50_06010"
     CDS             complement(3861..5162)
                     /locus_tag="PLP50_06010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51142.1"
                     /translation="MAEAPASSRFDVVVCGGGPGGSTTATLLARAGFSVALFERERFP
                     RFHIGESLLPWNVPLFRRLGVLEKLEATGPQVKYGARFYHQGTDHARLVRFADAFDGA
                     PASAFQVKRAELDTLLLDHARASGVAVSEQARVEEVLFEGERAVGVRVRVGDDAPREV
                     RAKAVVDATGRDALLSRRLGGRRKDPKLDRSAAFAHFSGFRRVDGPEGGDIVVVTTAD
                     GWWWLIPFSDGTVSVGVVMPSSRFANRPGTVEELFEARIAATPEVRDHLEGHSRLGEV
                     RAIPDYSYRTPRTSGDGFCLVGDAACFLDPVFSSGVLLAMSSGEMAAEAIGRALSRRG
                     RVDAADFRAMERRYQRAIGRFSSFVHGFYTPHVLETFYTDSPWPLVTGAVTTVLAGAV
                     FRPSLKSRLGTAAFRFASLLIRAKQKAAGAGPFERATGLLG"
     gene            complement(5155..6795)
                     /locus_tag="PLP50_06015"
     CDS             complement(5155..6795)
                     /locus_tag="PLP50_06015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51143.1"
                     /translation="MNRTGMVPDEAARGASAASDFDVVVIGGAIAGASTAILLRRRNP
                     ALRVLVVERSEEFDWKVGESTVEISSYFLIRVLKLYDHLSREQLPKQGFRYWFHNERA
                     TRVRDASEVGPTQLARTPSFQLDRAVLDGHLLRTAEEVGAEVWRPAKVVDLSLGGGDG
                     SSVTVEKPDGTKAVVRSRWIVDATGRQALLARRRGGVTPVESHPTSAIWVRYRGVKDL
                     DGTAVVGTDPADPFARGVVAARRLATNHFTGWGYWIWLIPLRGGETSVGLVWDKRLVQ
                     PEGAKPLEKLKRFLDSNPLTRELVEHAEPVDGDCRYYGHLPYFVDQFIGPGWTCVGDA
                     GGFLDPFYSPGLDQMSFSVHTRVDLIVKALEGTPAAEMERRYEDHNRRYDRFFRYFYE
                     SIYRDKYYIMGDYDTMTIAFLMDTALYYLVAVHPVYNESAARLGMPPFYGDGAELGLW
                     PMRFYARRLVAIAKRKKALGIYGNHNAGRRPRFVGFTLRTGAHVMLWHGLLRWAKAEL
                     ANAWTYVARPTPLEGPRPSLPPRAVAAPDGGAEAAAHG"
     gene            6984..9116
                     /locus_tag="PLP50_06020"
     CDS             6984..9116
                     /locus_tag="PLP50_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA51144.1"
                     /translation="MSDYRIQEHPILAIPGEAVVPFTWKGEALKARPGETIASALFAN
                     GVRIFGHHHKDGSPQGIFCANGQCAQCSVVANGLSVKSCMVRVAEGMAVEPLDGKAAL
                     LEADGDLRFHDVETVETECLILGGGPAGLAAAIELGKAGVNVVLVDDKAALGGKLVLQ
                     THKFFGSIEACHAGTRGLDIGKKLEAEVRSYPSVRIWTETTALAVFSDRKVGVLRDNH
                     YVLVRPQMLLVATGAREKSLVFKGNTLPGVYGAGAFQTLVNRDLVRPSERLFVVGGGN
                     VGLIGAYHALQAGIQVVGLCEALEECGGYKVHRDKLARMGVPIHTRHTIVSANGTEEV
                     ESVTIAKLDPSWKVIPGTEKTFACDTLLVAVGLDPVDEFLKKAREFGLPVVAAGDAEE
                     IAEASAAIFTGKIRGTEIARTLGRDVGEVPPEWLRTAEVLKARPGAVITEEIPADETG
                     VFPIFHCSQEIPCNPCTSICPKGAIKIEGDDILGLPTFDESACIACEQCVAICPGLAV
                     TLVDYRKKDAEGLLVSIPYEFPKARIKEGDMVTVLDTLGAVLGHVPVVKVRAPKFADH
                     ALLVKVKAPSEIAKRIAGIQVQEPWVSAAMEENVERIGDDDVVCRCERVTAGQIRQLI
                     REGVRDLNHLKALTRAGMGACGGKTCPTLIARIFREEGVPLAQVTPLTQRPVFMEVPL
                     GAFAGVVTGEGPTKDAVPTAVGALVGGV"
     gene            9116..10273
                     /locus_tag="PLP50_06025"
     CDS             9116..10273
                     /locus_tag="PLP50_06025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA51145.1"
                     /translation="MASSVYDVVVVGAGSVGMPTAMELAGKGLKVLCLDQFASAGQGS
                     NKAAIGGLRATHSSPAKIRLCLDSLKTFSTWKETHGFDIEWRQGGYMFVAYRPEEEKT
                     LKELLVVQKKAGLNIDWYAKDDLLKLAPGLDANGLLGGTFSPEDGSASPMLSSAAFHR
                     RAVELGVECRFNERVTAIMRRKGAVVGVRTDKGGYATSCVVNAAGAWARSLAHLGGQD
                     VPVNPDCHEAGITEPARRFLEPMVVDIRRTPGAANYYFYQAHSGQVIFCVTPDPPLIG
                     TDRRETSEFLPLIARRMVGLMPKLANLKVRRTWRGLYPMTPDGSPVVGRSAALRGYVD
                     AVGMCGQGYMLGPGVGALVARMVTETTTEDDAAILHELRPEREFAGTEKLK"
     gene            complement(10443..12185)
                     /locus_tag="PLP50_06030"
     CDS             complement(10443..12185)
                     /locus_tag="PLP50_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPA51146.1"
                     /translation="MTLRRGFLAFSLLAAHLVLADAAAASGHVYSRRFGSGSDQAVTA
                     VAVDGGGNAIVAGLFAGSIDFGGGSLTSAGGDDAFVAKLDPNGNHLWSRRFGDASAQS
                     ATGIAVDAIGNVLVTGYFSGTIDIGGATLTSAGGQDVFVAMFDSNGNHVWSKCFGNSS
                     NQAGAGVAVDPSGNVLLVGTFSGSIDFGGGNLTSAGSTDVFIARLDANGFHSWSKLFG
                     DASAQTGVAVAVDGDGNVFATGAFSGTVDFGGGGLTSAGSTDAFLVKLSAEGNYVWSK
                     RFGDASGQTGLALAVDASGGPILAGSFSGSVNFGGGALASAGSTDGFVAKLDSSGSHL
                     WSRALGGTGTDSANGVAVHGAGDVVVAGGFQASADLGGGTLTSAGGTDLFLARYTPGG
                     SWLTNAQAGDAAEQECRAVAVDASGFAIPAGRFQGSIDLGGGTLLSEGGHDAFAAKLG
                     VGTTLHTLAPCRLVDTRNAAGALGGPALEPGPSRRVFPVAGVCGIPATARSISVNVTV
                     TQPAAAGTLRVFPGDTAEEWTPTSTSISFSAQKTRANNSICLLSKDGAGSLAVRNDAP
                     GTVHFILDVSGYFD"
     gene            complement(12421..14376)
                     /locus_tag="PLP50_06035"
     CDS             complement(12421..14376)
                     /locus_tag="PLP50_06035"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51147.1"
                     /translation="MSRPAAPRAAQNLRGAILVALLLFASFAAQASEWSPVGPPGGAA
                     YGLALDPKDAKVLYAGAFGGGVWKSTDGGASWGRLSGIPAQETVNAVAVSPTDSRTVL
                     AAGFTALWRSTDGGATWKKVLDQKVQQPAMTGFAFDPAAPATVYASADNDGFPTGVFK
                     STDSGATWKPANVGINPNSRVRGVAVARDGKAVFAASEDGVYLSTDGGASWTVALPRK
                     AAHSVATGAGGLVLAGTNGDGVLRSTDGGKSWEESTTDAKWIGNIVYAIVASTATPGL
                     CYASIPNRVLRSTDGGATWKTFSRGFNWVNFRSLALDEKGGAVWAGSGRDGVVKTTDG
                     GATWTTGAGFLALEVKAILLDPASPKRLFVGTTQGGVHLSEDGGATWALSNEGLTNRA
                     VHSLAADPSAPGTFLAGTRKGAFRSTDGGRSWTHVLKGGCEPEVQHLRYAPSDPKRVY
                     AHSGRDFCQVARSDDGGLTWRDLKAPRDASTLLGHFAFHVDATNADRAFYSDDRHLYL
                     SADGGATFTTATGIPPTSRIQAIVAGAGAGELLAATNKGIYRSADGGKSWAVAGPGTE
                     KWNVRRILLDPASGTFYAGAWKEGVLRSKDGGKSWTRIGGEPPHPDVVALALEPNGTL
                     LVGFEGGGVWRLDGARSITPSAVTKPK"
     gene            complement(14697..15227)
                     /locus_tag="PLP50_06040"
     CDS             complement(14697..15227)
                     /locus_tag="PLP50_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51148.1"
                     /translation="MDAARVIALLRPHLEATGTRWMLAGGLALVAWGSTRTTADTDLL
                     VDDAFRSALLARLGAAGFEVLQDTDGFTNLLHFDREIGRLDLIWVEGETSRRLFAAAV
                     ERTGPDAIPLLVPAPEHLVAMKVKAMKEQPTRVFRDAEDLRLLLSLPDLDEDSVLATF
                     ERAGLGELHERIKGAS"
     gene            complement(15312..15782)
                     /locus_tag="PLP50_06045"
     CDS             complement(15312..15782)
                     /locus_tag="PLP50_06045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPA51149.1"
                     /translation="MTAAACPFRVELDVRFPEVDSYRVVWHGNYVLYCEVARNALCAA
                     AGLTPAEALACGWRVPITRFDVKVRRPARLDDRLEVSCTLRPPETAKLEMDYEVRRLP
                     DRLLLATGFTQQVVLSPQDELLLTFPKLLKELVGRILAYQRGERELTGEKILAG"
     gene            complement(15779..16885)
                     /locus_tag="PLP50_06050"
     CDS             complement(15779..16885)
                     /locus_tag="PLP50_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPA51150.1"
                     /translation="MREILVTGAAAVTALGRDLETTAARLAAGESAVAEVSVSEAGER
                     KGPAYAARIGSFTTEPEMPRAKARRLDRASQYAVVAVTRCVAEAGWVMAGNEEETGIL
                     LGTGSAGAGPLVDFERQMAVESPETASPFLFPYTVSNAPASVAAIELRVKGPNVTITQ
                     KDPAGLNALFYARFLLEDGRAKAIVAGAVDEWNLTYHLAYERLGVLKTATRPGYAMTE
                     GCSVVFLETAESARARGARPWARLAGAASSGAPVSPQTRRARPDDVAATLRAALADAG
                     VEPAAVGLVHASANGVPWIDAAEEAALADVFGTSPLPRVERVKLQAGENPCSGALQLA
                     LAARALRDDPSLGAVLVNALGAGGNQIAALFTAP"
     gene            complement(16896..18110)
                     /locus_tag="PLP50_06055"
     CDS             complement(16896..18110)
                     /locus_tag="PLP50_06055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPA51151.1"
                     /translation="MPDRTVVVTGLGAVTSLGNDLASTWAGLVAGRSGIGPITLFDAS
                     GFRTKLAAEVGELPEPGVSPRLRRRLSRTDLIGLTAAREALADAGLDLDREDLSRIGV
                     VLGGGVSGLLDSEAYFRDLLAKGPHGARPTRVLNHPPDQTTDRIAELWGFRGPRATIT
                     TACSSSSTSLGYAADLIRAGIADVVVTGGADSFARLTHGGFNALRACDPEPCRPFDRN
                     RKGLTIGEASGILVFEEEGRARRRGAAIRARFLGYGITSDAHHMTQPEPSGEAGARTI
                     RACLRSARLDPSDVDYVNAHGTATVQNDPAETLSIKLALGERARKICVSSNKSMLGHC
                     LCSAGAIEAVATVRTIETGIVPPTIHYVEPDPDCDLDVVPNTAREHPVRVALSNSFAF
                     GGNSTVVAFGRA"
     gene            18178..18612
                     /locus_tag="PLP50_06060"
     CDS             18178..18612
                     /locus_tag="PLP50_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPA51152.1"
                     /translation="MGERVDWNRYFMNIAAEVATRSTCPRKHVGAVIVRDRRILSTGY
                     NGSLRGLPHCDEAGCLMEDGHCIATVHAEANAILQAAMNGVRIEGGEIYTTASPCWNC
                     FKLIGNAGLVGICFSEFYREPRILEYAERMGIRLVDLGPGRR"
     gene            complement(18616..19290)
                     /locus_tag="PLP50_06065"
     CDS             complement(18616..19290)
                     /locus_tag="PLP50_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51153.1"
                     /translation="MHTILLVDDMRSFLDLEATFLSRADCRILTASTGLEAIRVARTE
                     KPDLIVLDIEMPEMNGIQACRILKSDPVTARILVVMLSSMKMEDEGRRAGADHFLRKP
                     IDEPTFLAEVKRFLPIVERAETRVVVDSPITFWRDGETFEARLTNLSRTGFYVAASRI
                     HPIGARLEVSFALPDDPEGKVVTGEALVVRHDDDPPGFGCRFRQVSSGARLVVEDWVE
                     RVAAGR"
     gene            complement(19332..21206)
                     /locus_tag="PLP50_06070"
     CDS             complement(19332..21206)
                     /locus_tag="PLP50_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51154.1"
                     /translation="MLRTVALILVLFASAPSLAVRPFEEEDAPSPVKAEVAVVASHAG
                     AGTEAATVAVLSREEIERLPARSLADLLRYLPSVDVRRRGPGVQADVSLRGADFNGTL
                     ILVDGEPVNDPQTNHHSLDLDVPADAIERIEVLYGSASALWGSEAVGGVIHIVTRGAD
                     LGRARAQIEGRYVHGSHSLDAGSVRVATRLSDSLTVSVDASRSESSGFRDDREHAEKT
                     VRASLRLETAAGPVTLSGGGASRAFGAWGFYGTRYPNQYEETRSRSLRLSADLSLGGG
                     WTLAPSASVRDHRDDFVLERTDPSFYRNLHDTDRSAVRLVARRPLLGGSLAIGGEAGR
                     DTIDSTNLGRRARSRSAAFVELARPFAAASPAAGGVRAGLRADRHEGFGTRLSPQLAA
                     WVGLGAGVKVRASAGTAFRVPTFTELHYVDPQTIGNPALKPEKSASYEAGLSWDHGAL
                     SFDAAVFLRHGTDLVDFVRSGPDEPYRATNLRTVDTRGLEATVVLDAARLAPTPLARL
                     AFRAAVYSADLEKLRAEAGATEGRYVLDPLRTRWDLLAEVRVPFRVEALARLSYFDRP
                     SFDEGVLLLDARLGYDVVDGDVVEVYLEGENLGAVRYEEVPGVPLPGRTLAAGVRLTW
                     "
     gene            21254..21829
                     /locus_tag="PLP50_06075"
     CDS             21254..21829
                     /locus_tag="PLP50_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51155.1"
                     /translation="MNAARAVVLALAGALLSLPASAQKPKPRAVLVEADGDEEALSAF
                     VARLESDLSDRGKATLSDARLAGISLGAVVAEPDGEAARVLRAEWAGAGWIGISLAPC
                     RVDVSRMTYRERSPEGYAYDRVVENVRVDCPATLRIVDSATGKEKKPLEVTGTANYRR
                     SGPDDEESPQLEATRDAAEKAAKKLPAALGR"
     gene            21947..22876
                     /locus_tag="PLP50_06080"
     CDS             21947..22876
                     /locus_tag="PLP50_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51156.1"
                     /translation="MRHPRHPSLLALAFLLAPATGFSLDLVSLPPGTGAAGIAVAPDG
                     TVWFSAQFSGALGRVSPSGEVSLFPFPSRDTQPLAVAVQPDGTAWSADLAGERLGRVA
                     PDGTFTEISTGRAGGEPTGLAVSADGIAWFTRPAEGRVGQRGSDGTVREFALPEASEP
                     TGIAVARDGAAWAVERRGNRLVRISPDGEITAWPLATPNAQPDAISIAPDGSVWAALP
                     NVSRIARVSSKGTLEEFAVDGAPAGIAVAPDGTVWFAAPTAKKLGRFLPGTGKVVWTP
                     LPDGPVAVSAAGDGTVWVVLGIGDKLGRIGPKE"
     gene            23046..23624
                     /locus_tag="PLP50_06085"
     CDS             23046..23624
                     /locus_tag="PLP50_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA51157.1"
                     /translation="MDAAILLFTNRGYAATSVREIVEMAGVTKPSLYYHFESKEGIYL
                     AILEELLKIADEGIAASRVKEGTARERLQAFLLGIFRVFETKKAEVRMMNAVFWGPAQ
                     GAPPFDFEIFHEKLKAVIAEIVAEGFAAGDLRAADPQDVTLALMGVLSFNMDLTLAHP
                     ELGLGQDGLRRTLDLVFEGLAAPRNAQQEPVR"
     gene            23621..24739
                     /locus_tag="PLP50_06090"
     CDS             23621..24739
                     /locus_tag="PLP50_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51158.1"
                     /translation="MNRTTLAAPSAALLLVLAAGAAGCGKPAAGNGSANGARPPAAVE
                     TAKAGTAPIEEAVEVVGSLTARSEADVKTEYTGTVAEVYVTQWVRVKAGTPLARLDTR
                     EADAGRQAAKAGALQAEAGAKRAARELDRTVKLKEAGLATQQQLDEARSAEEAARAAA
                     DAAKAQLALAETRLGKAVVRAPIDGVVALRNVNVGDYVENMGNPPPMFRVVDNRVLEL
                     TANVPSARMAALKVGQPFAFTSDALPGRTFSGKVSFINPAADEASRTVKVKAEVPNAD
                     GALKAGLFVKGRIVTGRREGVLVVPRAALVSWDTVARRGALYVVEGQVAKTVEVETGA
                     TAGDLVEVVKGISAGREVVTRGGFNLRDGDRVRVVRGA"
     gene            24743..29179
                     /locus_tag="PLP50_06095"
     CDS             24743..29179
                     /locus_tag="PLP50_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA51159.1"
                     /translation="MFLSDLSIKRPVFATVLMLALVTLGVFSYRRLAIDMMPDVEIPF
                     LSIVTQYPGASPETVEREVSKRIEQAVNPISGVKHVFSTSREGLSVVYVEFNLEVKIN
                     DVAQDARSKISAIRNDLPAGIEEPVIQKLDIGGMAVLSVAVRSERMTPRELTTLVDRK
                     VKRRLENLPGVGKVDLVGESLREVAVDLDPARLDALGLGVDEVVAGLAGENVNTPLGR
                     LSRGDQEMPVRVSGKPKEVDGFRSMVVATRGGVPIALGEVAEVRDTVEEQRKLALVSG
                     VPAVALDVYKQSKANTVGVVEAVTKELEELRAELPPSVDIQVVRDGSIMIKESVHDVT
                     NTLIIGAILTILIVFLFLNSWRSTVITGLTLPISVISSFIVMYFLGMTLNIMTLMALS
                     LAIGLLIDDAIVVRENIVRHLEQGQDHMTAARVGTAEIGLAVLATTLSIVAVFVPVAF
                     MKGIMGRFFFQFGITVAFAVLVSLFVSFTLDPMLSSRWVDPDVERIGNRHAVARALDR
                     FNDWFDRTADGYKVLIAWALDHRKAVLALATAAFVLGVGLFGALKSEFLGVYDQAEFQ
                     IKFRTAPGASFDETKGRMDAVLAELGKMPEVKHTYASIGAGDMGTVRDARVYVKLVEK
                     SERGKHATVLAAEARQRISGIAGIIPSVEMQSDDFAEKPVMVSIRGEEIPLLKKYAAE
                     LKKGALAIRGIEDVEVTLELDLPEYRLVVDRERAAASGLSTPAIARTVGALVGGQAVT
                     TYEDEEGEAVDVRVRLPLEMRRDVTQVGDLRIAVPGGRGGVPALVPLSELVKAERATS
                     PSEISRRDLSREVLLTANLDDLPLGAAVEQIREAAARIEMAPGYEIYFPGETERMEES
                     FGYMAEALLLAVVFVYLILAAQFESFIDPLSIMLSLPPSIVGMAGMLFLTGDTLSIMS
                     LIGLILLMGLVTKNAILLVDYTKVLRKGGMERREALITAGRTRLRPILMTTLAMIFGM
                     LPLALGLGQGAEMRAPLGRAVIGGLITSTVLTLLVVPVVYALFDDLALFIHRRWSKAN
                     ALEQEAHGHTGKAAAKATAATLLLALLVPAPLASAAEPASSGRRVLTLSDALSIAAEK
                     NRDVAKARAYQSWVRGKYVEERAGALPTVAATGSFGRSWDGTYAALTDGLFPSGQTVK
                     TAGVSLTQTLFAWGKVGAAVRAAKDGIASAEDQLEHYRQAAARDVTEAFHDVLLAKRI
                     EAIAKETLEQRERQLGEAERRHTLGTVTDYDVLAARVARDNQRPEVLRTASAVLVALD
                     RLRLVLAEESGEIDVDGALEAEAVELPSFEEAVATALEKRPDLSGLVRNAAIYRQLVR
                     IRNADDKPRLDLRANAGWQWFDAGVVSASGKVWGAGLYLSFPFFDGLATRGRVAQARS
                     DLTRAELDLAQARDGIHVEVRTALDRARVAAEIVNALSGNVAQARRLLEMSETGRELG
                     VKTRLEVDDALLNARAAEANLARAKRDYLVALADLRYAQGTL"
     gene            29211..29726
                     /locus_tag="PLP50_06100"
     CDS             29211..29726
                     /locus_tag="PLP50_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HPA51160.1"
                     /translation="MTTGRFAHLLVESPKAPPTEAELDAIAEAIGTPLPEELREWLAA
                     FNGGKLDYVFDLATEAGPFPVCFYTLYSTRRPSPARAAAGLVLHELEMERRLKELRPG
                     LLPIAGDGGTSVLYVDLAPPGTGQVVAYVEGLPGWDGTPDGCFVPVAPSLTAYAAGLY
                     LNTVEEPKRGG"
     gene            29793..30272
                     /locus_tag="PLP50_06105"
     CDS             29793..30272
                     /locus_tag="PLP50_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPA51161.1"
                     /translation="MKTIGAVVLVLSLGGGASLAEAGEARPGATRVAASAEVASRART
                     LLETQAAAWNRGDLEAFCSVYAEDALFLTAFGLTKGRAEVLARYRTRYPDAAARGTLS
                     FAVLSVDRLAGTPSEEAIAVVARWTISHPDRPAATGLTLLNLVRSGAGWKIVHDASM"
     gene            30340..30858
                     /locus_tag="PLP50_06110"
     CDS             30340..30858
                     /locus_tag="PLP50_06110"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HPA51162.1"
                     /translation="MRRTPVHVVAATVLATFVAAAEGPAPRPALRAAVAASLADVGWL
                     SGKWVVENGETYSEEWWSAPSGDSMVGAWRLSRSGRAKLFELLTLMEEERRVVLRIRH
                     FDAKGVAWEEKDRPLVLPLVEKGAGRAVFEGMEGDGLLRISYRRDAETLTVEIEKPEK
                     PLQRFRFGRAGS"
     gene            complement(30902..32470)
                     /locus_tag="PLP50_06115"
     CDS             complement(30902..32470)
                     /locus_tag="PLP50_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51163.1"
                     /translation="MPRHPSSLALVALLLVAPVAGAAPPSNDTCAAAITISGLELPFT
                     QQLETVQATEAGDPPMPCATASPGKSVWFKFEPATSDAYVFETGGSAPADYQPVLGLY
                     TGGCAGLTPVAGACARGRLTSYLEEGMTYYLMVAGDAVVVDPSIRIALDGVDLCPGGP
                     GPGGGGCGGAISVRVGDALSARAYNERNDSLLLIGGFSWDLGQNASPAGSTSPSVAFQ
                     YTAPVASTTVALTWTPPGQSPITRQLPISVGAAENLVATSDGPEAPAFQAAGETATLP
                     SPGGILRLSVQRDAPDWRFNSLIPSVASTKNAAGVPFVSDLSLTNLESTETLVGLELW
                     TAAGRREASPIRLAPKGSRTVSDVVKSAFGLEQTFGTLYISSTGAIVAGARTWAPFAG
                     GTTGQFALAGDVRTSASPALLRTGEVGVLPGVRQDSAFRTNIGVYNVSDKECVVELEA
                     RDEDGQTVGSKLALTLPPTRYAQEPLARLTTNLPSGSVTLTNGTSGCYVGGVAYVIDN
                     VTQDPFAVSQRKKP"
     gene            32563..34764
                     /locus_tag="PLP50_06120"
     CDS             32563..34764
                     /locus_tag="PLP50_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPA51164.1"
                     /translation="MSRTAWGAIALVGVLSAPPARAQPVESAAERPTVGLVLSGGSAH
                     GLAHVGVLKVLHELRIPVDVVTGTSMGSIVGGLYAAGMSPPEMEWLMGTIDWEDILDD
                     RPSRDRLSFRRKQDEMPSYVDLEMGLTTRGLAFPSGLIAGQKLGFLLKSLTLGTVGIE
                     EFDDLPIPFRCIATDIATGEKVVLAGGSLAEAIRASMSIPGVFSPVPRGDRLLVDGGL
                     VDNLPVGEARDLGADVVIAVDVSSDKFDMEKLKSFGGVLSRMTSLPVVRNVSVSAPRA
                     NVVLTPRVGDIPSMGFSRAAEIVARGEEEARRLAKVLSALSVPEEEYRAHRERIRGRR
                     VDRIIVDEIQTVSVGVDPRQVRARLESRPGKPLDVGTLRRDLDRIYEMGVFETVEFRI
                     LRDEGRHVLVIDARPKSWGPTFVRVGSGFEADFDGDATVALRGTLHAMQLNSRGGEVK
                     ATLELGTVPGLNVEYYQPLDFRGRTFFSVDGSFVRTLARVSTPDGLVGEGRITLLQGS
                     VDAGLSLGSWGQVRAGVFRGAGRGEPLVTNVPIDDLELDVGGLFAQASVDTLDDLSFP
                     RRGTLAIAAVEAYRPDLGSESSFNRLILRGTQAISVGRRTALHLTGGWEDTMGSHPPF
                     YLLFSLSDFTRLAALSREEATGERAAYAAAVVQRRIASLPTRVGRGVYLGGAFEAGRV
                     WARREDVGFDGIRPAGGLFVGADTVLGPIYAGFGVGYGGNTTLYVFLGRPY"
     gene            complement(34928..35716)
                     /locus_tag="PLP50_06125"
     CDS             complement(34928..35716)
                     /locus_tag="PLP50_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51165.1"
                     /translation="MKSVTAAAALAALVSATPLAHASEPTVVWYPSVVNTEGLNGARY
                     YSVLSVWNPSPAEITLTVDGFVSNSSGLAAPTATVTATVPGATHATIPNVLASLFGGL
                     EGLAAARITASGPATFFHSVVNDQTAAGNGRTLLKVDNARGENVLAGGTLLQLSNASA
                     EDRAKGLGKRTNVGYFNPSFDTKSVRFRAWTPNATLIGEEVRSIPAWSQVQQPVFELI
                     PPTTAGDSAHEDFLVTFEVEGGGLLVYSIVVDNKTNDGVFQAAE"
     gene            35981..>38405
                     /locus_tag="PLP50_06130"
     CDS             35981..>38405
                     /locus_tag="PLP50_06130"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPA51166.1"
                     /translation="MNARSTSFDALIAAALLLAAVSSPAAPVPLSNGGFEQAAASRAA
                     SWSVVGTLPPAAIVVRDAEVKRAGAASLRLSATSPASITVASDEVRLEVGHVYRLSAF
                     VRTENAVSDPTGRYPTPVAATLAMESFPFTNHSPSAGATRPFGKVETLFVATTARDRV
                     RLQLGRNGAATGTAWFDDVTLEEVTDVRELVPMETVRWHGPAFRYDEKGWIYVHVEGE
                     PYERGFQYGSLVAEELAEYVKKLGIQENPKDGPGAWAEMRFFADAVFLRKYDEEYLLE
                     MKGIADGAAKAGAKVDGRPVDFLDVVTMNSSIDLDYAKSALRSTPHPLTGRSFLTSED
                     EAKIPERAHKCSSFVATGEATKDGRPVFGQIFMWAGYTGVHFNVIADVVPSKGHRLVY
                     QTYPGGIHSGTDYYMNAAGILIGETTVAQTPWNVDGTPMSNRIRKAAQYASSIDDVAR
                     ILREKNNGMYTNDWPIVDVKTGEVAILLLGTKESKLWRTGEDPAPFGTPGFLWANNNA
                     RDPGVKREYGVQPDDAPFDPVYGPWDRDVAFRKWYDEVKGRIDATEAVNLWASSPINR
                     PHACDGKVTTAEMAEKLVFLAHYGKVTLREKFPAAGNRRMPDHPGAIPHLTLGYAVFS
                     PVYIAEKLKEAKAKEPKAAAAPKEPALDVTAVEERLAYDATKLWKGSVFPKTEGTNGL
                     VSGAAAYWRLLKGLPKEPARAFASVRDELAGLNARAAWFAAKEGDVAIREATREYGRR
                     SPYQIPRIKGTFALHQLRLLLGNERFRAATDAAFTANAGREVTAEELVATLSKGAGRD
                     VAPFLAPWIE"
BASE COUNT         5508 a        13275 c        14209 g         5413 t
ORIGIN      
        1 agtcggtgta gacgggggcg cggcgccgct tctcgatgag cgagacgagg ccgcgaaggt
       61 gcttgcgggc cgtctcgagc atcgtcggcg tcacgtcctg ccaccactcg tccgactgca
      121 tcgcctggat caggacgatc cgctcgcgga ccatcggaat cgcggccttc tcctcgagga
      181 ggccgcagat cgcgcggacc tgctcgctca gcctctggaa tgacggctcg tgcctgagga
      241 cggagagctg gaggtggagc atcaggaggt cgaaccactt cgcctcctcc tcctcggatg
      301 cgaggccgct cgggaggccc gccacctcgg cggccagctc ctgccggtcg acgttcgaaa
      361 gctccgtcca ggccacgggc tcgcggtact tctcgacgag ccggcgcttc ggtcggacga
      421 cgaagctctc gaggctcatc gaggaaacct cggcgtggag gagcgccgcc acctcgccgc
      481 ggagggctgt gtcgtcggcg cggcggctcg cgaggttcgt ttcgtcgatc gggctcgagc
      541 ccggtatggc cggaacgggc gcgaggcgct cgtccagctc gccgatcagg tcgaggcgcc
      601 gaaggaagag gaggtaggtg atctgctcga tgacctcgag cgggttcgag atgcctcccg
      661 accagaaggc gtcccagatg cggtcgacct agctgcgaag ctcgccggtg agcacgttcc
      721 gtccccctgc gcggcccgac ggcgaaaagc cgagcgggcg aatggaaggc cggggggagt
      781 ctacccgtcg tctcctcgac aggcctcccc gcgggtggga tcatccccgg atttcaggcc
      841 tgccgctcga gcggcgggcc ccaggacgga gcccctcttg aagaagctcg tggaaaccct
      901 ctcctccctc aagctcgccg tcggcctcct cgtcgtcctc ctgatcgggc tgtcgatcgg
      961 gacgatcctc gagtcgcgga ccgacgcggc gacggcgcag cgggtcgtct actactcgtg
     1021 gtggttcctc gccttgcagg ggctcttcgc gctgaacgtc gcctgctcgc tcgcgaacct
     1081 cttcccgtgg gggaagaagc gggccgggtt cgtcacgacg cacgcggcgc tccttctcat
     1141 cctggcgggg gcgacgctca cctatttctg gaaggtggag gggcagctcg gcctctggga
     1201 gggggagagc ggcgcgacga tcgagaacca cgacgcgcag gggaacgtcg tctcccggca
     1261 cgacctgccg ttctcggtga agcttgtcga cttcgtcctc gacacctacc cggggacgat
     1321 gcggccggcc ggattcgcga gcgaggtcgt cattaccgac accgacaccg ggaagtcgtt
     1381 cccggcgcgg atctggatga accacccgct ccaccaccgc gggtacacgc tcttccagtc
     1441 gagctaccag cagcaggggg ggcgcgaggc gacggtgctc tcggtctcga aggacccggg
     1501 gcagctcatc gtcttcgccg ggtacgtcgc ggtcgtcctc gggatgatcc tcgtcctcgt
     1561 gacgcgcgtc tcgcaggcgc gggagcggga ccgcgagagg gccgcgctcg aggcgaaggt
     1621 cgcagagggc ggcatgtcgc tcccgacgat ggggaaggcg ctcctcctcg ccctcgcgct
     1681 cctgccgctc gcggcgccgg cggcgacgcc ggagctcgag gcgctccgcc ggctcccggt
     1741 gcagcacgac ggccggacga tgccgctcga cacgctcgcg cgagagacgg tctggaacgt
     1801 gacggggtcg cacgccttcc gcggggagga cccggcggcg acgttcaccg gctggctctt
     1861 cgacccgcgc tcggccgcga cgacgccggt cgtgaaggtg ggcagcaagg cgctggcggc
     1921 ggcggcgggg ctcccggcgg ggacgaagca cgtctcgttc gagcagctgg tctcgaacca
     1981 gcgcctcatg gggctgatcc gccaggcggg gatggccgcg cagcgggagg agccgcgccg
     2041 gggcgtcctg aaggacgccg agaagctcga gaaccggctg aacgtcctct acgggctcct
     2101 ccagcgggag accgtccggc cgatcccggc ggcggaggga ccgaaggcgc gctggaaccg
     2161 ccccgaggcc gtgacgaagg aggcgctcct cgccctcgcg aacggcccgc gcctcgcggg
     2221 ctggccgacg gcgaagaaga tcgacggcga gatcctctac aaccgcgtca accccgcgaa
     2281 gctctcctgg atcgtcctcc tcgcctcgct cgtcgtctcg atcgtcgcct ggtcgaaggc
     2341 gagcaaggcc ctcgactgga cctcgttcgc cctcctcgcg gtcggcttcg gcgtgatgac
     2401 gtggggcatc gggatgcggt gggccgcggg ggagcggatc ccggcggcga acatgtacga
     2461 gtcgctcctc ttcctcgcgt ggggcgtcgg cctcttcgcg gtgatcgcct acctggtgat
     2521 gcgtaaccgg accgtcgtcc tgaacgccgc ggcgatgtcg gcgctgacga tggcgctcac
     2581 cgacctcctc ccgatggacc gcttcatcca cccgatcgcg cccgtcctgg ccgggacgcc
     2641 gtggctcgcg atccacgtcc cgatcatcat ggtcggctac gcggtcctcg cgctcggcgt
     2701 cgtcgtcgcg cacatgcagc tcgcgttcgc ggcgttcggg aaggggaagg gggcgctcgt
     2761 cgagaggctc tacgagctcc tctactggta catgcacgtc gggtcgatcc tcctcctcgc
     2821 ggggatcctg accggctccg tctgggccgc ctcgtcgtgg ggccgctact ggggctggga
     2881 cccgaaggag gtctggtcgc tcgtcgcgtt cctcgcgtac atggcgatcc tccacctgaa
     2941 ggtcgacaag gtgatcggga cgttcggggc cgcggccatc agcatcctcg cgttccagac
     3001 gatcctgatg acgtacctcg gagtgaacta cgtcctgaac gtcggcatgc actcctacgg
     3061 catgggcgac tcgccgatcg tgaagtggat ggttctcgtc gcgctcgtcg aggtcgtctt
     3121 cctcgccgtc tgcggcgtgg cgtaccgcga ccggaggagg atgctcgcgg aggcctgagg
     3181 acggggagcc cgagcggagc gccgcgaccc gggagggtgc ggcgctccgg cgggaggttc
     3241 agcccttctt ccgcgccagg tggaggacga gcggcggctc ctcggtcttg ccgcccttcc
     3301 agccgatcca ggtctcgcgg atctcgtcct ctccgaggag ctcgaccttc acggcgtgga
     3361 tgtgcacgtc cttcgcggaa tcgaggttcg tgccgccgtc gaactccatc gcgatcacgt
     3421 tcgccggcac cgtcttcgtc cggaaccgcg gctggttttg cgccgcacag tagtgcgtca
     3481 tcacgaggtc gtttccgtcg cggtgccaca ccgagaccat ctcgtgaggg gtgccgggag
     3541 cgagcgtcga gacgaccgcc gagccggcgc ccgtgacgcg gtacatcacg gcgacggccc
     3601 ccttctcccc gaccttcggc tcgatctcga cccagtcgcc ggcgagcgcc ttcagcttct
     3661 cgagggcggc ggcgccggac gtgggcgggg cggacggggg gggggggggg gcgcggcctt
     3721 cttcggctcc tcggcgcgga ggggaagggg gagggcgagg agggagccga cgccgacggc
     3781 gaagaggagc ttccgaatgc gggacgcggg cgacttcatg gaggcctcct tgtcgatctg
     3841 gcaggatcgt acctgcggcg ctacccgagg agtccggtcg ctctctcgaa ggggccggca
     3901 ccggcagcct tctgcttcgc ccggatcagg agcgaggcga agcggaacgc cgcggtgccg
     3961 agccgtgact tcaggctcgg gcggaagacg gcgccggcga ggacggtcgt gacggcgccc
     4021 gtgaccagcg gccagggcga gtcggtgtag aacgtctcga ggacgtgcgg cgtgtagaag
     4081 ccgtggacga agctgctgaa gcggccgatc gcacgctggt accgcctctc catcgcgcgg
     4141 aagtccgccg catcgacccg cccgcggcgc gagagcgccc ggccgatcgc ctccgcggcc
     4201 atctccccgg aggacatcgc gaggaggacg ccgctcgaga agaccggatc gaggaagcag
     4261 gccgcgtctc cgacgaggca gaagccgtcc cccgaggtgc gcggcgtccg gtaggagtag
     4321 tcggggatcg cgcgcacctc gccgaggcgc gagtgccctt cgaggtggtc acggacctcg
     4381 ggcgtcgcgg cgatccgcgc ctcgaagagc tcctcgaccg tccccggccg attcgcgaag
     4441 cgggacgacg gcatcacgac gccgacggag acggtcccgt cggagaacgg gatgagccac
     4501 caccagccgt cggccgtcgt gacgacgacg atgtcgccgc cttccggccc gtcgaccctc
     4561 cggaagccgg agaagtgggc gaacgcggcc gagcggtcga gcttcgggtc cttccggcgc
     4621 ccgccgagcc ggcgcgagag gagggcgtcc cgcccggtgg cgtcgaccac cgccttcgcc
     4681 ctcacctcgc ggggcgcgtc gtccccgacc cggacgcgga cgccgaccgc ccgttcgccc
     4741 tcgaagagga cctcctcgac tcgcgcctgc tcgctcaccg cgacgcccga ggcgcgggcg
     4801 tgatcgagga ggagcgtgtc gagctccgcc cgcttcacct ggaaggccga ggccggtgcg
     4861 ccgtcgaagg cgtcggcgaa cctcacgagg cgggcatggt ccgtcccctg gtggtagaag
     4921 cgggcgccgt acttcacctg cggcccggtc gcctccagct tctcgagcac gccgagcctt
     4981 cggaagagcg ggacgttcca cggcaggagc gactcgccga tgtgaaagcg cgggaagcgc
     5041 tcccgctcga agagcgcgac ggagaagccg gcgcgtgcga ggagcgtcgc cgtcgtcgag
     5101 ccgcccggcc ccccgccgca gacgacgacg tcgaagcgag aggaggcggg cgcctcagcc
     5161 atgcgcggcg gcctcggcgc ccccgtccgg tgcagccacg gcgcgcggcg ggagcgaagg
     5221 gcgcggtcct tcgagcggcg tcggccgcgc gacgtacgtc caggcgttcg cgagctcggc
     5281 cttcgcccac cggaggaggc cgtgccacag catgacgtgc gcgccggtgc ggagcgtgaa
     5341 gccgacgaag cgcggccgtc gcccggcgtt gtggttgccg tagatcccga gcgccttctt
     5401 ccgcttcgcg atcgcgacga ggcggcgcgc gtagaagcgc atcggccaga ggccgagctc
     5461 cgcgccgtcg ccgtagaacg ggggcattcc gagccgggcc gccgactcgt tgtagacggg
     5521 gtggaccgcg acgaggtagt agagggccgt gtccatcagg aaggcgatcg tcatcgtgtc
     5581 gtaatcgccc atgatgtagt acttgtcgcg gtagatcgac tcgtagaagt agcgaaagaa
     5641 gcggtcgtag cgccggttgt ggtcctcgta cctccgctcc atctctgcgg ccggcgtccc
     5701 ctcgagcgcc tttacgatca ggtcgaccct cgtgtggacg gagaacgaca tctggtcgag
     5761 ccccggcgag tagaacggat cgaggaagcc gccggcgtcg ccgacacagg tccaccccgg
     5821 cccgatgaac tggtcgacga agtaggggag gtggccgtag tagcggcagt ccccgtcgac
     5881 gggctcggca tgctcgacga gctcccgcgt gagcgggttc gagtcgagga atcgcttcag
     5941 cttctcgagc ggtttcgcac cctcgggctg aacgagtcgc ttgtcccaca cgaggcccac
     6001 gctcgtctct ccgccgcgga gcgggatgag ccagatccag tagccccagc cggtgaagtg
     6061 gttcgtcgcg aggcgtcggg ccgcgacgac gccgcgcgcg aaggggtcgg ccgggtccgt
     6121 cccgacgacc gcggtgccgt cgaggtcctt cacgccccgg tagcggaccc agatggccga
     6181 ggtcggatgg ctctcgacgg gcgtgacgcc gccgcgccgc cgtgcgagga gcgcctggcg
     6241 cccggtggca tcgacgatcc atctcgaccg gacgacggcc ttcgtcccgt cgggcttctc
     6301 gacggtgacg gagctcccgt ccccgcctcc gagagagagg tcgacgacct tcgccggcct
     6361 ccagacctcg gcccccacct cctccgcagt ccggaggagg tggccgtcga ggacggcccg
     6421 gtcgagctgg aagctcggcg tgcgcgcgag ctgcgtcggc ccgacctccg aggcgtcacg
     6481 cacccgcgtg gccctctcgt tgtggaacca gtatcggaag ccctgcttcg gcagctgctc
     6541 ccggctcagg tggtcgtaga gcttcaggac gcggatcagg aagtacgagg agatctcgac
     6601 cgtcgactcg cccaccttcc agtcgaactc ctcgcttctc tccacgacga ggactcggag
     6661 ggccggattg cggcgacgaa ggaggatcgc ggtggaggcc cccgcgatcg cgccgccgat
     6721 gacgacgacg tcgaagtccg aggccgcgga cgcgccgcgg gccgcctcat ctgggaccat
     6781 gccagtcctg ttcattcgcg gatcatagag agaggacgga cgagatcggg gaggaaacga
     6841 cgcgagaagg gcttctcctc tctggacgcc gacccccatc ctccttatcc tcgcctgttg
     6901 ggccgccccc gagagggcgc gcccgacccc cctcccggcg accgcggtcc cgcggcgccg
     6961 gcgagccggg gacgaggagc ctcatgtcgg actacaggat ccaggaacac ccgatcctcg
     7021 cgatcccggg cgaggcggtc gtgcccttca cgtggaaggg cgaggcactg aaggcgaggc
     7081 cgggcgagac gatcgcctcg gccctcttcg cgaacggggt gcgcatcttc gggcaccacc
     7141 acaaggacgg ctcgccgcag gggatcttct gcgcgaacgg ccagtgcgcc cagtgctcgg
     7201 tcgtggcgaa cggcctgagc gtcaagtcgt gcatggtgcg cgtcgccgag gggatggccg
     7261 tcgagccgct cgacggcaag gccgccctcc tcgaggcgga cggcgacctc cgcttccacg
     7321 acgtcgagac ggtggagacg gagtgcctga tcctcggcgg cggcccggcc ggcctcgccg
     7381 cggcgatcga gctcgggaag gcgggcgtga acgtcgtcct cgtcgacgac aaggccgccc
     7441 tcggcggcaa gctcgtcctc cagacgcaca agttcttcgg ctcgatcgag gcgtgccacg
     7501 ccgggacgcg cggcctcgac atcggcaaga agctggaggc ggaggtccgc tcctacccga
     7561 gcgtgaggat ctggaccgag acgacggccc tcgccgtctt ctccgaccgg aaggtcggcg
     7621 tcctgcgcga caaccactac gtcctcgtcc ggccacagat gctcctcgtg gcgacgggcg
     7681 cccgcgagaa gtcgctcgtc ttcaagggga acacgctccc cggcgtctac ggcgcggggg
     7741 cgttccagac gctcgtgaac cgcgacctcg tgaggccctc cgagcggctc ttcgtcgtcg
     7801 gcggcgggaa cgtcggcctc atcggcgcct accacgcgct ccaggcgggg atccaggtcg
     7861 tcggcctctg cgaggcgctc gaggagtgcg gcggctacaa ggtccacagg gacaagctcg
     7921 cgcggatggg ggtcccgatc cacacgcgcc acacgatcgt ctcggcgaac gggacggagg
     7981 aggtcgagag cgtcacgatc gcgaagctcg acccgtcctg gaaggtgatc ccggggaccg
     8041 agaagacgtt cgcgtgcgac acgctcctcg tcgccgtcgg cctcgacccg gtggacgagt
     8101 tcctgaagaa ggcgagggag ttcggcctgc cggtcgtggc ggcgggcgac gcggaggaga
     8161 tcgccgaggc gagcgcggcg atcttcacgg ggaagatccg cgggaccgag atcgcgcgga
     8221 cgctcggccg cgacgtcggc gaggtgccgc cggagtggct gcggacggcc gaggtcctga
     8281 aggctcgccc cggcgccgtc atcacggagg agatccccgc cgacgagacg ggcgtcttcc
     8341 cgatcttcca ctgctcccag gagatcccgt gcaacccctg cacctcgatc tgcccgaagg
     8401 gggcgatcaa gatcgagggg gacgacatcc tgggcctccc gacgttcgac gagagcgcct
     8461 gcatcgcctg cgagcagtgc gtcgcgatct gccccggcct cgcggtgacg ctcgtcgact
     8521 accggaagaa ggacgccgag gggctcctcg tttcgatccc gtacgagttc cccaaggcca
     8581 ggatcaagga aggcgacatg gtcaccgtcc tcgacacgct cggcgccgtc ctcgggcacg
     8641 tgccggtcgt gaaggtccgc gcgccgaagt tcgcggacca cgccctcctc gtgaaggtaa
     8701 aggccccctc cgaaatcgca aagcgaattg cagggatcca ggttcaggag ccgtgggtct
     8761 cggcggcgat ggaggagaac gtcgagcgga tcggcgacga cgacgtcgtc tgccgctgcg
     8821 agcgggtcac agccggacag atccgccagc tcatccgcga gggggtgcgg gacctcaacc
     8881 acctgaaggc gctgacgcgc gccgggatgg gcgcctgcgg cgggaagacc tgccccacgc
     8941 tcatcgcgcg gatcttccgc gaggaggggg tgccgctcgc ccaggtgacg ccgctgacgc
     9001 agcggcccgt cttcatggag gtgccgctcg gcgccttcgc gggggtcgtg acgggcgagg
     9061 gcccgacgaa ggacgcggtg ccgacggcgg tcggcgcgct cgtgggaggc gtctgatggc
     9121 ctcgtccgtg tacgacgtcg tcgtcgtcgg cgccgggagc gtcggcatgc ccaccgcgat
     9181 ggagctcgcc ggcaaggggc tgaaggtcct ctgcctcgac cagttcgcct ccgccgggca
     9241 ggggagcaac aaggccgcca tcgggggact ccgcgccacg cactcctcgc ccgcgaagat
     9301 ccggctctgt ctcgactcgc tgaagacgtt ctccacctgg aaggagacgc acggcttcga
     9361 catcgagtgg cggcagggcg gctacatgtt cgtcgcctac cgccccgagg aggagaagac
     9421 gctgaaggag ctcctcgtcg tccagaagaa ggcgggcctc aacatcgact ggtacgcgaa
     9481 ggacgacctc ctgaagctcg cgcccggcct cgacgcgaac ggcctcctcg gcggcacctt
     9541 ctcgcccgag gacggctcgg cctcgccgat gctctcctcc gcggccttcc accgccgcgc
     9601 ggtcgagctg ggtgtcgagt gccggttcaa cgagcgggtg accgcgatca tgcggaggaa
     9661 gggcgccgtc gtcggcgtcc gtaccgacaa ggggggctac gccacctcct gcgtcgtgaa
     9721 cgccgccggg gcctgggcgc ggagcctcgc gcacctcggc gggcaggacg ttccggtgaa
     9781 ccccgattgc cacgaggcgg ggatcaccga gccggcccgg cggttcctcg agccgatggt
     9841 cgtcgacatc cggcggacgc cgggcgcggc gaactactac ttctaccagg cgcactcggg
     9901 gcaggtcatc ttctgcgtca cgcccgaccc gccgctgatc gggaccgacc ggcgcgagac
     9961 gtcggagttc ctgccgctca tcgcgcggcg gatggtcggc ctgatgccga agctcgcgaa
    10021 cctgaaggtc cgccgcacgt ggcgcggcct ctacccgatg acgcccgacg gctcgcccgt
    10081 cgttgggagg agcgccgcgc tccgcggcta cgtcgacgcc gtcgggatgt gcgggcaggg
    10141 ctacatgctc ggcccgggcg tcggcgccct cgtcgcgcgg atggtgacgg agacgacgac
    10201 ggaggacgac gccgcgatcc tccacgagct gcggccggag agggagttcg cggggacgga
    10261 gaagctgaag tagggaaccc tcccccgaac tcctcctctc ccctgccgcg cccccgtcgt
    10321 ctgggagctc tgggtcgacg tctgggcgtg acacggatcc gggccggagg gcctcggctg
    10381 gctcccggtg ggcgtgacga actccctgct cggagcgtgt gacgcaggcg ccgccttcgg
    10441 cctcagtcga agtagccgct cacgtcgagg atgaagtgga ccgtccccgg cgcgtcgttc
    10501 ctcaccgcca ggctgccggc cccgtccttc gagagaaggc agatggagtt gttcgcgcgg
    10561 gtcttctggg cgctgaacga gatcgaggtc gaggtggggg tccactcttc cgccgtgtcg
    10621 ccggggaaga cccgaagcgt tcccgcggcc gcgggctgag tcacggtgac gttgacggag
    10681 atcgagcgcg cggtcgccgg gatgccgcag acaccggcga cgggaaagac ccgtcgcgaa
    10741 gggccgggct cgagagccgg ccctcccagg gccccggcgg cgttgcgcgt gtcgacgagg
    10801 cggcagggcg ccagcgtgtg gagcgtcgtc ccgacgccga gcttggcggc gaaggcatcg
    10861 tgaccgccct cgctcaggag cgttccgccg ccgaggtcga tgctgccctg gaagcggccc
    10921 gcgggaatcg cgaagcccga agcgtcgacg gcgaccgccc ggcactcctg ctcggcggcg
    10981 tcgccggcct gcgcgttcgt gagccagctc ccgcctgggg tgtagcgggc caagaagaga
    11041 tccgtaccgc ccgcgctcgt gagcgttccc ccgccgaggt ccgcgctcgc ctggaaccca
    11101 ccggcgacga cgacgtctcc tgccccgtgc acggcgacgc cgttggcgga atcggtgccg
    11161 gtgccgccga gggcgcggga ccagagatgg ctcccggagg aatcgagctt cgcgacgaac
    11221 ccgtcggtcg atcccgcgct cgcgagtgcc ccgcccccga agttcaccga cccgctgaac
    11281 gaacctgcga ggatcgggcc tccggacgcg tcgacggcca gggcgagtcc cgtctggccc
    11341 gaggcgtcgc cgaaccgctt gctccacacg tagtttccct ccgctgagag ctttacgagg
    11401 aaggcgtccg tcgagccggc gctcgtcagg ccccctccgc cgaaatccac ggtcccggag
    11461 aaggcgcccg tcgcgaagac gttcccgtcg ccgtcgacgg cgacggcgac gcccgtctgc
    11521 gcgctcgcgt caccgaagag cttgctccag gagtggaacc cgttcgcatc gagcctcgcg
    11581 atgaagacgt ccgtcgagcc ggcgctcgtc aggttccctc ccccgaaatc gatcgacccg
    11641 gagaacgtcc ccaccaggag gacgttcccc gacgggtcga ccgcgacgcc cgcgcccgcc
    11701 tggttgctcg agttcccgaa acacttgctc cagacgtggt tcccgttcga gtcgaacatc
    11761 gcgacgaaga cgtcctgccc gcccgcgctc gtgagcgtcg cgcctccgat gtcgatcgtg
    11821 ccgctgaagt acccggtcac caggacgttt ccgatcgcgt cgacggcgat gccggtcgcg
    11881 ctctgggcgc tggcgtcccc gaaccggcgg ctccagagat gattcccgtt cgggtcgagc
    11941 ttcgcgacga aggcgtcgtc gccccccgcg ctcgtcagcg agccgccgcc gaaatcgatg
    12001 gagcccgcga acagtccggc gacgatcgcg ttcccgccgc cgtcgacggc cacggccgtg
    12061 accgcctggt cggagccgct gccgaagcgg cggctgtaga cgtgcccgga cgccgccgcg
    12121 gcgtcggcga ggaccagatg cgcggccagc agcgagaagg cgaggaaccc tcgacgaagc
    12181 gtcatctctg caccgtcttc ccggacgggt ccgccaggtc gctcgcccgc cggccggact
    12241 gcctctcgtg ccggcccgac ccttcgggag aggaagaccc gcataccgac ctggcgttct
    12301 ccggttccgg tgcacgagga tataccgcga cgcagcaatc gcgcgtcggc gctcgagctt
    12361 cgcgggcctc ggccggtggt gggtcgagac ggtcggaagg aaccgcgaat ccggaggggt
    12421 ctacttcggt ttcgtgacgg ccgacggggt gatggagcgc gccccgtcga ggcgccagac
    12481 gcccccgcct tcgaagccga cgagcagcgt tccgttcggc tcgagtgcga gggcgacgac
    12541 gtcggggtgc gggggctcgc cgccgatccg tgtccagctc ttgccgccgt ccttcgagcg
    12601 gaggactccc tccttccacg ccccggcgta gaacgtgccg gaggccgggt cgaggaggat
    12661 ccgcctcacg ttccacttct cggtgccggg ccctgccacc gcccagctct tcccgccgtc
    12721 ggccgagcgg tagatcccct tgttcgtcgc ggcgaggagc tcgccggccc cggccccagc
    12781 gacgatcgcc tggatgcggg aggtgggcgg gatgccggtc gcggtcgtga acgtcgcgcc
    12841 gccgtcggcg gagaggtaga ggtgccggtc gtcgctgtag aaggcgcggt cggcgttcgt
    12901 cgcgtcgacg tggaacgcga agtggccgag gagcgtgctc gcgtcgcggg gcgccttcag
    12961 gtcgcgccag gtgaggccgc cgtcgtcgct ccgcgcgacc tggcagaagt cgcggccgct
    13021 gtgagcgtag acgcgcttcg ggtcggaagg ggcgtagcgg aggtgctgca cctccggctc
    13081 gcacccgccc ttgaggacgt gcgtccagct ccggccgccg tccgtcgacc ggaaggcgcc
    13141 cttgcgggtc ccggcgagga acgtcccggg agcggaggga tcggcggcga gcgagtggac
    13201 cgcgcggttc gtgagcccct cgttcgagag cgcccacgtc gcgccgccgt cctcggaaag
    13261 gtggacgcct ccctgagtcg ttccgacgaa gaggcgcttc ggcgaggcgg gatcgaggag
    13321 gatcgccttc acctcgagcg ccaggaaccc ggcgccggtc gtccaggtcg ctccgccgtc
    13381 cgtcgtcttc acgacgccgt cgcggccgct cccggcccag accgcgccgc ccttctcgtc
    13441 gagggcgagc gagcggaagt tcacccagtt gaagccgcgg gagaacgtct tccacgtggc
    13501 gccgccgtcg gtcgagcgga ggacgcggtt cgggatgctc gcgtagcaga ggccgggcgt
    13561 cgccgtcgag gcgacgatcg cgtagacgat gttcccgatc cacttcgcgt cggtcgtcga
    13621 ctcttcccag ctcttcccgc cgtcggtcga gcggagcacc ccgtcgccgt tcgttcccgc
    13681 gaggacgaga ccgcccgcgc ctgtcgcgac ggagtgcgcg gccttcctcg gaagggcgac
    13741 cgtccaggac gccccgccgt ccgtggagag gtagacgccg tcctcggagg ccgcgaagac
    13801 ggccttcccg tcccgcgcga cggcgacgcc ccggacgcgc gagttcgggt tgatcccgac
    13861 gttcgccggc ttccacgtgg cgccggaatc ggtcgacttg aagacgcccg tcgggaagcc
    13921 gtcgttgtcg gcgctcgcgt agacggtcgc cggtgcggcc gggtcgaacg cgaagccggt
    13981 catcgcgggc tgctgcacct tctggtcgag gaccttcttc caggtcgccc cgccgtcggt
    14041 cgagcgccag agggcggtga aaccggccgc gaggaccgtc cgggagtccg tggggctgac
    14101 cgcgacggcg ttcacggtct cctgcgcggg aatgcccgag aggcggcccc agctcgcccc
    14161 gccgtcggtc gacttccaga cgccgccgcc gaacgccccc gcgtagagga ccttcgcgtc
    14221 cttcgggtcg agcgcgaggc cgtacgccgc gccgccgggc gggccgacgg ggctccactc
    14281 ggaggcctgt gccgcgaagc tcgcgaacag caggagagcg acgaggatcg ctccgcggag
    14341 gttctgcgcg gctctcgggg cggcgggtcg gctcatgcgg cctccttgcg gcagtgccgc
    14401 gggggaggtc tctccccgcg ggcttacggt taccggacga agaacgcgga gagtaagcgg
    14461 ccgggggcgc gaaggggaag ggtcccgtgc gagggggggg gcggggcgcg gctcaggcgt
    14521 cgagcgtgaa ccgagggccc cgggcgaggg ggatcgcccg gagctgctcc gcggtccatc
    14581 ggaactgcct caggaagcgg gcgtactccg gcagcgacat cggcccgggg cgatgtctct
    14641 cgagcgaatc ggcgacggcg ggagacgccg acggaagcgc gtcgagatcg aggggctcag
    14701 gacgcgccct tgatccgctc atgaagctca ccgagaccgg cccgctcgaa cgtcgcgagg
    14761 acggaatcct cgtccagatc gggaagggag aggaggagcc gaaggtcctc ggcgtcgcga
    14821 aacacgcggg tcggctgctc cttcatcgcc ttcaccttca tggcgacgag gtgctccggg
    14881 gcggggacga ggagaggaat tgcgtccggg ccggtcctct cgacggcggc ggcgaacaac
    14941 cggcggctcg tctcgccttc cacccagatc aggtcgagcc ggccgatctc gcggtcgaag
    15001 tgcaggaggt tcgtgaagcc gtcggtgtcc tggaggacct cgaatcccgc tgctccgagg
    15061 cgcgcgagga gggcggagcg gaaggcgtcg tcgacgagga ggtccgtgtc ggccgtcgtg
    15121 cgtgtggagc cccaggccac gagggccagg ccacccgcga gcatccaccg cgtcccggtc
    15181 gcctcgaggt ggggcctcag gagcgcaata acgcgtgccg cgtccattcg ggcgaactct
    15241 acgccgacgg ggcgtcgacg ggctaccggc gccggactca ggggaaagcg cggaatccgc
    15301 tctcgcgggg cctatccggc caggatcttc tctcccgtca gctcccgctc cccgcgctgg
    15361 taggcgagga tgcgcccgac gagctccttc aggagcttcg ggaacgtgag gaggagctcg
    15421 tcctgcgggg agaggacgac ctgctgcgtg aagcccgtgg cgaggaggag ccggtcgggg
    15481 aggcggcgga cctcgtagtc catctcgagc ttcgccgtct cgggcgggcg gagcgtgcag
    15541 gagacctcga ggcggtcgtc gaggcgtgcg gggcggcgga ccttcacgtc gaagcgggtg
    15601 atcggcaccc gccacccgca cgcgagcgcc tccgccggcg tgaggcccgc cgcggcgcag
    15661 agggcgttgc gcgccacctc acagtagagg acgtagttgc cgtgccagac gacgcgatag
    15721 gagtcgacct ccgggaaccg gacgtcgagc tcgacgcgga aggggcacgc ggccgccgtc
    15781 acggcgcggt gaagagggcg gcgatctggt tgccgccggc gccgagcgcg ttgacgagga
    15841 cggcgccgag cgacgggtcg tcgcgaaggg cgcgcgcggc gagggcgagc tggagggcgc
    15901 cggagcaggg gttctcgccc gcctggagct tgacccgctc gacgcgcggc agcggcgagg
    15961 ttccgaagac gtctgcgagg gccgcctcct cggccgcgtc gatccagggg acgccgttcg
    16021 cggaggcgtg gacgagcccg accgccgccg gctcgacgcc ggcgtcggcg agcgcggcgc
    16081 ggagcgtcgc ggcgacgtcg tcgggccggg cgcggcgcgt ctgcggggag acgggggcgc
    16141 cgctcgaggc ggcgccggcg agccgggccc aggggcgcgc gccgcgcgcg cgggccgact
    16201 ccgcggtctc gaggaagacg acggagcatc cttcggtcat cgcgtagccc gggcgcgtgg
    16261 cggtcttcag gactccgagc cgctcgtacg cgaggtggta cgtgaggttc cactcgtcga
    16321 ccgcgcccgc gacgatcgcc ttcgcgcgtc cgtcctcgag gaggaagcgg gcgtagaaga
    16381 gggcgttcag gccggccggg tccttctggg tgatcgtgac gttcggtccc ttgaccctca
    16441 gctcgatcgc ggcgacggac gcgggggcgt tcgagacggt gtacgggaag aggaacggcg
    16501 aggccgtctc gggcgactcg acggccatct gccgctcgaa gtcgacgagg ggcccggcgc
    16561 cagcggagcc ggtgccgagg aggatgccgg tctcctcctc gttgccggcc atgacccagc
    16621 cggcctcggc gacgcagcgg gtgacggcga cgacggcgta ctggctcgcg cggtcgaggc
    16681 ggcgggcctt cgcccgcggc atctcgggct ccgtcgtgaa ggagccgatg cgggcggcgt
    16741 acgcgggccc cttcctctcg cccgcctcgg agacggaaac ctccgcgacg gcgctctcgc
    16801 cggcggcgag gcgcgccgcg gtcgtctcca gatcgcgccc gagcgcggtg acggcggcgg
    16861 cgccggtgac gaggatctct ctcacggcgc gctcctcagg cccgtccgaa cgcgacgacg
    16921 gtggagttgc cgccgaacgc gaaggagttc gagagggcga cgcggaccgg gtgctcccgc
    16981 gccgtgttcg ggacgacgtc gaggtcgcag tcggggtccg gctcgacgta gtggatcgtg
    17041 ggggggacga tccccgtctc gatcgtccgg accgtcgcga cggcctcgat cgctcctgcg
    17101 gagcagaggc agtgtccgag catcgacttg ttcgaggaga cgcagatctt ccgtgctctc
    17161 tcgccgaggg cgagcttgat cgagagcgtc tcggccgggt cgttctggac cgtcgcggtg
    17221 ccgtgggcgt tcacgtagtc gacgtcggag gggtcgaggc gcgcggagcg aagacaggcg
    17281 cggatggtcc gcgcccccgc ctcgcccgag ggctcgggct gcgtcatgtg gtgggcgtcg
    17341 ctcgtgatcc cgtagccgag gaagcgggcg cgaatcgccg cgccgcggcg tcgggcacgg
    17401 ccttcctcct cgaagacgag gatccccgac gcctccccga tcgtgagccc cttccggttc
    17461 cggtcgaagg gccggcacgg ttcggggtcg caggcgcgga gggcgttgaa gccgccgtgc
    17521 gtgaggcggg cgaaggaatc ggccccgccg gtgacgacga cgtcggcgat ccccgcgcgg
    17581 atcaggtccg ccgcgtagcc gagcgaggtc gacgaggagg agcaggcggt cgtgatcgtc
    17641 gcgcgcgggc cgcggaaacc ccagagctcg gcgatccggt cggtcgtctg gtcggggggg
    17701 tggttcagga cccgggtcgg gcgggccccg tgcggcccct tcgcgaggag gtcgcggaag
    17761 tacgcctcgc tgtcgaggag gcccgagacg cccccgccga ggacgacgcc gatccgcgac
    17821 aggtcctccc ggtcgaggtc gaggccggcg tcggcgagcg cctcgcgcgc ggccgtcagg
    17881 ccgatgaggt cggtccggga gaggcgacgc cggaggcgcg gcgagacgcc cggttcgggc
    17941 agttcgccca cctcggcggc gagcttcgtc cggaagccgc tcgcgtcgaa gagggtgatc
    18001 gggccgatcc cgctccggcc ggccacgagg ccggcccagg tggaggcgag gtcgttcccc
    18061 agggaggtga cggcgccgag gccggtgacg acgacggtcc tgtcgggcat gggccgaatc
    18121 ctacacgcgc ccgccgcggc gcagcgacgc ccgccccgcg ggccgcataa tcgggcgatg
    18181 ggcgagcgcg tcgactggaa ccggtacttc atgaacatcg cggcggaggt ggcgacccgc
    18241 tccacctgcc cgcgcaagca cgtcggcgcg gtcatcgtcc gggaccggcg gatcctctcc
    18301 acgggctaca acggctctct ccggggcctc ccgcactgcg acgaggccgg ctgcctgatg
    18361 gaggacgggc actgcatcgc cacggtccac gccgaggcga acgcgatcct ccaggcggcg
    18421 atgaacggcg tccgcatcga ggggggcgag atctacacga ccgcctctcc ctgctggaac
    18481 tgcttcaagc tgatcgggaa cgccggcctg gtcgggatct gcttctcgga gttctaccgc
    18541 gagccgcgaa tcctcgaata cgccgaacgg atggggatcc gactcgtcga cctcggcccc
    18601 gggcggcgct gagcttcagc gtcccgcggc gacgcgctcg acccagtcct ctacgacgag
    18661 ccgtgcgccc gaggagacct ggcggaagcg gcatccgaag ccgggcgggt cgtcgtcgtg
    18721 gcggacgacg agcgcctcgc ccgtgacgac cttcccctcc gggtcgtccg gcagcgcgaa
    18781 cgacacctcg agccgcgccc cgatcgggtg gatccgggac gccgccacgt agaagccggt
    18841 ccgcgagagg ttcgtcaggc gcgcttcgaa cgtctcgccg tcgcgccaga acgtgatcgg
    18901 cgagtcgacg acgacgcgcg tctcggcccg ctcgacgatc ggcaggaagc gcttcacctc
    18961 ggcgaggaac gtcggctcgt cgatcggctt gcggaggaaa tggtctgcgc ccgcgcggcg
    19021 tccctcgtcc tccatcttca tcgaggagag catgacgacg aggatgcggg cggtgacagg
    19081 gtcggacttc aggatgcgac acgcctggat gccgttcatc tcgggcatct cgatgtcgag
    19141 gacgatcagg tcgggcttct ccgtccgggc gacccggatc gcctccaggc cggtcgaggc
    19201 ggtcaggatc cggcagtcgg cgcgcgagag gaacgtggcc tcgaggtcga ggaagctcct
    19261 catgtcgtcg acgagcagaa tcgtgtgcat cgaaccggta cgccctcgct tccccgagtt
    19321 tacgctccgc ctcaccaggt gaggcgcacg cccgcggcca gggtccttcc ggggagcggc
    19381 acgcccggca cctcctcgta gcggacggcc ccgaggttct ccccctcgag gtagacctcg
    19441 acgacgtcgc cgtccacgac gtcgtagccg aggcgggcgt cgaggaggag gactccctcg
    19501 tcgaaggagg gccggtcgaa gtacgagagg cgcgcgagcg cctcgacgcg gaacgggacg
    19561 cggacctccg cgaggaggtc ccagcgcgtc cgcagcgggt cgaggacgta gcgcccctcg
    19621 gtcgcgcccg cctcggcccg gagcttctcg aggtcggccg agtagacggc ggcccggaac
    19681 gcgagccggg cgagcggcgt cggggcgagg cgcgccgcgt cgaggacgac ggtcgcctcg
    19741 aggccccgcg tgtcgacggt gcggaggttc gtggcccggt acggctcgtc cgggccggaa
    19801 cggacgaagt cgacgaggtc cgtgccgtga cggaggaaaa cggccgcgtc gaacgagagg
    19861 gcgccgtggt cccaggagag gcccgcctcg tacgacgccg atttctcggg tttcagcgcg
    19921 gggttgccga tcgtctgcgg gtcgacgtag tggagctcgg tgaacgtcgg gacccggaac
    19981 gcggtgcccg ccgaggcgcg gaccttcacg ccggccccga gcccgaccca cgccgcgagc
    20041 tgcggcgaga ggcgcgtccc gaagccctcg tgccggtcgg cgcggaggcc tgcacgcacc
    20101 ccgccggcgg ccggggacgc cgcggcgaac ggccgggcca gctcgacgaa cgcggcgctc
    20161 cgggagcgtg cgcgtcgtcc gaggttcgtg gagtcgatcg tgtcgcgccc cgcctctccc
    20221 ccgatcgcga gggagccgcc gaggagcggg cgtcgcgcga cgaggcggac ggcggagcgg
    20281 tcggtgtcgt ggaggttccg gtagaaggag gggtcggtcc gctcgaggac gaagtcgtcc
    20341 cggtggtcgc gcacggaggc cgaaggggcg agcgtccagc cgccgccgag ggagaggtcg
    20401 gccgagaggc gcaggctccg gctcctcgtc tcctcgtact ggttcggata gcgcgtgccg
    20461 tagaaccccc aggcgccgaa ggcccggctc gcgccgcctc ccgagagggt gaccgggccg
    20521 gccgcggtct cgagccgcag cgaggcgcgg accgtcttct cggcgtgctc ccggtcgtcg
    20581 cggaagccgc tcgactcgga gcgggaggcg tcgaccgaga ccgtgaggga gtcggagagc
    20641 ctcgtggcga cccggacgct cccggcgtcg agcgagtggg agccgtggac gtatcggcct
    20701 tcgatctggg cgcgcgcgcg gccgaggtcc gcgccgcgcg tgacgatgtg gatcacgccg
    20761 ccgaccgcct cggagcccca gagcgccgag gcggagccgt agaggacctc gatccgctcg
    20821 atggcgtcgg ccgggacgtc gaggtccagc gagtggtggt tcgtctgggg gtcgttgacc
    20881 ggctccccgt ccacgaggat cagcgtgccg ttgaagtcgg ccccgcggag ggagacgtcg
    20941 gcctggaccc cgggtccgcg gcgcctcacg tcgacggagg ggagataccg gaggaggtcg
    21001 gccagcgagc gcgccgggag ccgctcgatc tcctcccggc tgaggacggc gaccgtcgcc
    21061 gcctccgtcc ccgcccccgc gtgcgaggcg acgacggcca cctccgcctt cacgggggag
    21121 ggcgcgtcct cctcctcgaa cggccgcact gcgagcgacg gagcagaggc gaagaggacg
    21181 aggatgagcg cgaccgtgcg gagcatgcgg cgcggaggat agcggacgcg gcccatcgcg
    21241 ctactcttcc gtcatgaacg ccgctcgcgc cgtcgtcctc gcgctcgccg gggccctcct
    21301 ctcgctcccc gcgtccgccc agaagccgaa gccgcgggcc gtcctcgtgg aggccgacgg
    21361 cgacgaggag gcgctctccg cgttcgtggc ccgcctcgag tcggacctct ccgatcgagg
    21421 gaaggcgacg ctgtcggacg cccgcctcgc cgggatctcc ctcggcgcgg tcgtcgcgga
    21481 gccggacggc gaggccgccc gcgtcctgcg tgcggagtgg gccggagcgg gctggatcgg
    21541 catctcgctc gccccgtgcc gcgtggacgt cagccggatg acctaccgcg agaggagccc
    21601 ggagggatac gcctacgacc gggtcgtgga gaacgttcgg gtggactgcc cggcgacgct
    21661 ccggatcgtc gactccgcca ccgggaagga aaagaagccc ctggaggtca ccggcaccgc
    21721 gaactaccgg aggagcggtc ccgacgacga ggagagcccg cagctcgagg cgacccgcga
    21781 cgccgccgag aaggcggcga agaagctccc ggctgcgctc ggccgctgag ggcgccgccg
    21841 gaagctggcc ccgccggggc gaagagatac ttcgcgcggg tttcatgccc tgggcctatc
    21901 ctcggctgtt cgggcgcccg gcacgcggcg tccgcggcgg ttcgacatga ggcaccctcg
    21961 acacccgtct cttctcgcgc tcgcgttcct cctcgctccg gccaccggct tctctctcga
    22021 cctcgtcagc ctgccgccgg gaaccggggc ggcggggatc gccgtcgctc ccgacgggac
    22081 cgtctggttc tcggcgcagt tctccggggc gctcggccgc gtgagcccgt ccggcgaggt
    22141 ctcgctcttc ccgttcccct cgcgcgacac ccagccgctc gcggttgccg tccagccgga
    22201 cggcacggcg tggagcgccg acctcgccgg cgagcgactc ggccgcgtcg ctccggatgg
    22261 caccttcacg gagatctcca ccggaagagc gggaggggag ccgacggggc tcgccgtgtc
    22321 cgcggacggg atcgcgtggt tcacgcggcc ggccgagggg cgcgtcgggc agcgcggctc
    22381 ggacgggacg gtgcgcgagt tcgcccttcc ggaggcgagc gagccgaccg ggatcgccgt
    22441 cgcgcgagac ggcgctgctt gggccgtcga aaggagaggc aaccgcctcg tgcgcatctc
    22501 ccccgacggg gagatcacgg cctggccgct cgcgacgccg aatgctcagc ccgatgcgat
    22561 ctccatcgcg ccggacgggt ccgtctgggc ggccctgccg aacgtgagcc gcatcgcccg
    22621 ggtctcgtcg aaggggaccc tcgaggagtt cgccgtcgac ggcgctccgg ccggaatcgc
    22681 cgtggcgccg gatggaacgg tctggttcgc ggccccgacg gcgaagaagc tcgggcggtt
    22741 ccttccgggg acgggcaaag tcgtctggac gccgctcccg gacgggccgg tcgccgtctc
    22801 ggcggccggg gacggaacgg tctgggtcgt cctcggcatc ggcgacaagc ttgggcggat
    22861 cgggccgaag gagtaggcgc gccgccgggt ctcctctttt tctggtcgta gcccttgacg
    22921 aacgctttct gcggtcccag attacctacc ggtcggtagc tcgatccggt ggacggatag
    22981 gggctgccgg ggaggttcga tccgtgccgc aagaacctgg tgaaagctct gtccgcaaaa
    23041 gacttatgga cgccgcgatc ctcctcttca cgaatcgcgg ctatgccgcg acctcagtcc
    23101 gggagattgt ggagatggcg ggtgtgacca agccgtcgct ctactaccac ttcgagagca
    23161 aggaggggat ctacctcgcg atcctcgagg agctcctgaa gatcgccgac gaggggatcg
    23221 ccgccagccg ggtgaaggag ggcaccgcga gggagcgcct ccaggcgttc ctcctcggga
    23281 tcttccgggt cttcgagacg aagaaggccg aggtccggat gatgaacgcg gtcttctggg
    23341 ggccggccca gggcgcgccg ccgttcgact tcgagatctt ccacgagaag ctcaaggcgg
    23401 tcatcgccga gatcgtcgcg gagggattcg ctgcgggcga cctccgcgcg gccgacccgc
    23461 aggacgtcac cctcgccctc atgggcgtcc tctccttcaa catggacctc accctcgcgc
    23521 acccggagct cgggctcggg caggacggcc tccgccggac gctcgacctc gtcttcgagg
    23581 gcctcgccgc cccccgcaac gcacagcagg agcccgttcg atgaaccgca cgaccctcgc
    23641 cgccccgtcc gcagcccttc tcctcgtcct cgccgccggc gccgccggct gcggcaagcc
    23701 ggccgccgga aacggctcgg cgaacggagc gcgtccgccc gccgccgtgg agacggcgaa
    23761 agccggcacg gccccgatcg aggaggccgt cgaggtcgtc ggaagcctga ccgcccggag
    23821 cgaggccgac gtgaagaccg agtacaccgg gaccgtcgcc gaggtctacg tcacccagtg
    23881 ggtgcgcgtg aaggcgggga cgccgctcgc ccggctcgac acccgggagg ccgacgccgg
    23941 ccggcaggcg gcgaaggcgg gcgcgctcca ggccgaggcc ggcgcgaagc gcgccgcccg
    24001 ggagctcgac cggacggtga agctgaagga ggcggggctc gccacgcagc agcagctcga
    24061 cgaggcgcgc tccgcggaag aggcggcgcg agccgcggcc gacgcggcga aggcccagct
    24121 cgccctcgcc gagacgcgcc tcgggaaggc cgtcgtgagg gcgccgatcg acggcgtcgt
    24181 cgcgctccgg aacgtgaacg tcggcgacta cgtcgagaac atggggaacc cgccgccgat
    24241 gttccgggtc gtcgacaacc gcgtgctcga gctgacggcg aacgtcccgt cggcgcggat
    24301 ggcggccctg aaggtgggcc agccgttcgc gttcacgagc gacgcgctcc cggggcggac
    24361 cttctccggg aaggtctcct tcatcaaccc cgcggccgac gaggcgagcc ggacggtgaa
    24421 ggtgaaggcc gaggtgccga acgccgacgg cgcgctgaag gccgggctct tcgtgaaggg
    24481 acggatcgtc accggccgaa gagagggcgt cctcgtcgtg ccgcgggccg ccctcgtctc
    24541 ctgggacacg gtcgcccggc ggggcgcgct ctacgtcgtc gagggccagg tcgcgaagac
    24601 ggtcgaggtg gagacgggcg cgacggccgg agacctcgtc gaggtcgtga aggggatctc
    24661 cgcgggacgg gaggtcgtca cgcgcggagg cttcaacctg agggacgggg accgcgtccg
    24721 ggtcgtccgg ggagcctgag ccgtgttcct ctcggacctc tccatcaaga ggcccgtctt
    24781 cgcgacggtc ctcatgctcg ccctcgtcac gctcggcgtc ttctcgtacc ggcgcctcgc
    24841 gatcgacatg atgccggacg tcgagatccc gttcctctcg atcgtcacgc agtaccccgg
    24901 ggcctcgccg gagaccgtcg aacgggaggt ctcgaagcgg atcgagcagg ccgtcaaccc
    24961 gatctcgggc gtgaagcacg tcttctcgac ctcgcgcgag gggctctcgg tcgtctacgt
    25021 cgagttcaac ctcgaggtga agatcaacga cgtcgcgcag gacgcgcgct cgaagatctc
    25081 ggcgatccgc aacgacctcc cggccgggat cgaggagccg gtcatccaga agctcgacat
    25141 cggcgggatg gcggtcctct cggtcgccgt ccggtccgaa aggatgacgc cgcgcgagct
    25201 gacgacgctc gtcgaccgga aggtcaagcg gcgcctcgag aacctccccg gcgtcgggaa
    25261 ggtcgacctc gtcggcgaga gcctccggga ggtcgcggtc gacctcgacc ccgcgcgcct
    25321 cgacgccctc gggctcggag tggacgaggt cgtcgccggc ctcgcgggcg agaacgtgaa
    25381 caccccgctc ggccggctga gccgcggcga ccaggagatg ccggtccgcg tctccggcaa
    25441 gccgaaggag gtcgacgggt tccggtcgat ggtcgtcgcc acgcgcggcg gcgtaccgat
    25501 cgcgctcggc gaggtcgccg aggtgaggga caccgtcgag gagcagcgga agctcgcgct
    25561 cgtcagcggc gtcccggccg tcgcgctcga cgtctacaag cagagcaagg cgaacacggt
    25621 cggcgtcgtc gaggcggtga cgaaggagct cgaggagctc cgcgccgagc tcccgccgtc
    25681 ggtcgacatc caggtcgtcc gcgacggctc gatcatgatc aaggagtcgg tccacgacgt
    25741 cacgaacacg ctgatcatcg gcgcgatcct gacgatcctc atcgtcttcc tcttcctcaa
    25801 ctcctggcgc tcgaccgtca tcaccgggct gacgctcccg atctccgtca tctcctcgtt
    25861 catcgtcatg tacttcctcg ggatgacgct gaacatcatg acgctcatgg cgctgtcgct
    25921 cgcgatcggg ctcctgatcg acgacgcgat cgtcgtgcgc gagaacatcg tccggcacct
    25981 cgagcagggg caggaccaca tgacggcggc gcgggtcggg acggccgaga tcggcctcgc
    26041 cgtcctcgcg acgacgctct ccatcgtcgc cgtcttcgtc ccggtcgcct tcatgaaggg
    26101 gatcatgggg cgcttcttct tccagttcgg gatcaccgtc gcgttcgcgg tcctcgtctc
    26161 cctcttcgtc tccttcacgc tcgacccgat gctctcctcg cgctgggtcg acccggacgt
    26221 cgagcggatc gggaaccgcc acgccgtggc gcgcgccctg gaccgtttca acgactggtt
    26281 cgaccggacg gccgacggct acaaggtgct catcgcctgg gcgctcgacc accggaaggc
    26341 ggtcctcgcg ctcgcgacgg ccgccttcgt cctcggcgtc gggctcttcg gggcgctcaa
    26401 gagcgagttc ctcggcgtct acgaccaggc cgagttccag atcaagttcc ggaccgcgcc
    26461 tggcgcctcg ttcgacgaga cgaaggggcg gatggacgcc gtcctcgccg agctcgggaa
    26521 gatgcccgag gtgaagcaca cctacgcctc gatcggcgcg ggggacatgg ggaccgtgcg
    26581 cgacgcgcgg gtctacgtga agctcgtcga gaagtcggag cggggcaagc acgcgaccgt
    26641 cctcgccgcc gaggcgcggc agaggatctc cgggatcgcc gggatcatcc cgtccgtcga
    26701 gatgcagagc gacgacttcg cggaaaagcc ggtcatggtc tcgatccgcg gcgaggagat
    26761 cccgctcctg aagaagtacg ccgccgagct caagaagggg gccctcgcga tccgcgggat
    26821 cgaggacgtc gaggtgacgc tcgagctcga cctcccggag taccggctcg tcgtcgaccg
    26881 cgagcgcgcg gccgcctccg gcctctccac cccggcgatc gcccggacgg tcggcgccct
    26941 cgtgggcggg caggccgtga cgacgtacga ggacgaggag ggggaggcgg tcgacgtccg
    27001 cgtccggctc ccgctcgaga tgcgccgcga cgtgacgcag gtcggagacc tccggatcgc
    27061 cgtccccggc gggcggggcg gggtccccgc gctcgtgccg ctctcggagc tcgtgaaggc
    27121 cgagcgggcg acgtcgccct ccgagatctc gcggcgcgac ctctcgcgcg aggtcctcct
    27181 gaccgcgaac ctcgacgacc ttccgctcgg ggcggccgtc gagcagatcc gcgaggcggc
    27241 ggcgaggatc gaaatggccc ccggctacga gatctacttc cccggcgaga ccgagcggat
    27301 ggaggagagc ttcggctaca tggccgaggc gctcctcctc gcggtcgtct tcgtctacct
    27361 catcctcgcg gcgcagttcg agtcgttcat cgacccgctc tcgatcatgc tgtcgctccc
    27421 gccctcgatc gtcggcatgg ccgggatgct cttcctgacc ggcgacacgc tcagcatcat
    27481 gtcgctcatc ggcctcatcc tcctgatggg gctcgtcacg aagaacgcga tcctcctcgt
    27541 cgactacacg aaggtcctcc ggaagggcgg catggaacgc cgcgaggcgc tcatcacggc
    27601 ggggcggacg cggctccggc cgatcctgat gacgacgctc gcgatgatct tcggcatgct
    27661 gccgctcgcc ctcgggctcg gccagggggc cgagatgcgg gcgccgctcg gccgggccgt
    27721 catcggcggc ctcatcacct cgaccgtcct gacgctcctc gtcgtcccgg tcgtctacgc
    27781 gctcttcgac gacctcgccc tcttcatcca ccggcgctgg tcgaaggcga acgccctcga
    27841 gcaggaggcg cacgggcaca cggggaaggc ggcggcgaag gccacggccg cgacgctctt
    27901 gctcgccctg ctcgtcccgg cccctctcgc atcggctgcg gagcccgcct cgagcggccg
    27961 ccgcgtcctg acgctctccg acgccctctc gatcgccgcg gagaaaaacc gcgacgtcgc
    28021 gaaggcgcgc gcctaccaga gctgggtccg gggcaagtac gtcgaggagc gcgcgggagc
    28081 gctcccgaca gtcgcggcga cgggctcctt cggccgctcg tgggacggga cctatgccgc
    28141 gctgacggac gggctcttcc cctcggggca gaccgtgaag acggcgggcg tctccctcac
    28201 gcagacgctc ttcgcgtggg ggaaggtcgg ggcggcggtc cgggccgcga aggacggcat
    28261 cgcctcggcc gaggaccagc tcgagcacta ccggcaggcc gcggcccggg acgtgaccga
    28321 ggcgttccac gacgtcctcc tggcgaagag gatcgaggcg atagcgaagg agaccctcga
    28381 gcagcgcgag cgccagctcg gggaggcgga gcggcgccac acgctcggga ccgtcaccga
    28441 ctacgacgtc ctcgccgcac gggtggcccg cgacaaccag cgcccggagg tgctccggac
    28501 ggcgagcgcc gtcctcgtgg cgctcgaccg gctgaggctc gtcctcgccg aggagtcggg
    28561 agagatcgac gtggatgggg cgctcgaagc ggaggccgtg gagctcccct ccttcgagga
    28621 ggcggtggcg acggcgctcg agaagcgccc ggacctctcc ggtctcgtcc ggaacgcggc
    28681 gatctaccgc cagctcgtcc ggattcgaaa cgccgacgac aagccgcggc tcgacctccg
    28741 cgcgaacgcc ggatggcagt ggttcgacgc gggggtcgtc tcggcctcgg gcaaggtctg
    28801 gggggcgggc ctctacctct ccttcccgtt cttcgacggc ctggcgacgc gggggcgcgt
    28861 ggcgcaggcg cggagcgacc tgacgcgggc cgagctcgac ctcgctcagg cgcgcgacgg
    28921 aatccacgtc gaggtccgga cggcgctcga ccgggcgcgg gtcgcggcgg agatcgtgaa
    28981 cgccctctcg gggaacgtcg cgcaggcgcg gcggctcctc gagatgtcgg agacggggcg
    29041 cgagctcggc gtaaagacgc gcctcgaggt ggatgacgcc ctcctgaacg cccgcgcggc
    29101 cgaggcgaac ctcgcccgcg cgaagaggga ctacctcgtc gcgctcgccg acctccggta
    29161 cgcgcaggga acgctctgac ggggcggggc gcgtcgggga gaatggcgcg atgacgacgg
    29221 gacggttcgc gcatctgctc gtcgaatcgc cgaaggcgcc gccgaccgag gccgagctcg
    29281 acgcaatcgc ggaggcgatc gggacgccgc ttccggagga gctccgggag tggctcgccg
    29341 cgttcaacgg cggcaagctc gactacgtct tcgacctcgc gacggaggcc gggccgttcc
    29401 ccgtatgctt ctacaccctc tattcgaccc gccggccgtc tcccgcgcgg gccgccgccg
    29461 gcctcgtcct ccacgagctc gagatggagc ggcgcctgaa ggagctccgg ccgggcctgc
    29521 tgccgatcgc gggggacggc ggcacgtcgg tcctctacgt cgacctcgcg ccgccgggga
    29581 cggggcaggt cgtggcgtac gtcgaagggc tgccgggatg ggacgggacg ccggacggct
    29641 gcttcgttcc ggtcgccccg agtcttaccg cgtacgccgc gggcctctac ctgaacaccg
    29701 tggaggagcc gaagcgcgga gggtagggag cgcgggaggc cggcgccgtc ccgcccgtcc
    29761 ccccggggag cgaaggcgga tacgattcgg acatgaagac gatcggagcg gtagtcctgg
    29821 tcctctctct gggcggaggc gcgtccctcg cggaggccgg ggaggctcga cccggcgcca
    29881 cccgggtcgc cgcgtcggcg gaggtcgcct cccgggcgcg gacgctcctc gagacccagg
    29941 cggcggcctg gaacaggggc gacctcgagg ccttctgctc ggtctacgcc gaggacgccc
    30001 tcttcctgac ggcgttcggc ctgacgaagg gacgcgcgga ggtcctggcg cgctaccgga
    30061 cgcgctatcc cgacgccgcc gcgcgaggga cgctctcgtt cgcggtcctc tcggtcgacc
    30121 ggctcgcggg gaccccttcg gaggaggcga tcgcggtcgt cgcccgatgg acgatctcgc
    30181 atcccgaccg cccggcggcg acggggctca cgctcctcaa cctcgtgcgg agcggcgcgg
    30241 gctggaagat cgtccacgac gcctcgatgt gatcggaccg ctcgctcacc gggcccgggc
    30301 tccgatcggg gcgagcgggg ccttcggcag gagacaggga tgagaagaac gcctgtgcac
    30361 gtcgtcgccg cgaccgtcct cgcgaccttc gtcgccgccg ccgaggggcc ggcgcctcgc
    30421 ccggccctcc gcgcggcggt cgccgcctct ctcgcggacg tgggctggct ctccggcaaa
    30481 tgggtcgtcg agaacggcga aacgtactcc gaagagtggt ggtcggcgcc ctcgggcgac
    30541 tcgatggtcg gagcctggcg cctctcgcgg tccggacgcg cgaagctctt cgagctgctg
    30601 acgctgatgg aagaggagcg gcgggtcgtc ctccggatcc gtcacttcga cgcgaagggc
    30661 gttgcgtggg aggagaagga tcgccccctc gtcctgccgc tcgtcgagaa gggggcgggg
    30721 cgcgccgtct tcgaggggat ggaaggggat gggctgctcc gcatctcgta tcgccgcgac
    30781 gcggagacgc tgacggtcga gatcgagaag ccggaaaagc cgctccagcg cttccgcttc
    30841 ggccgggcgg ggagctgagc ccccgggggc gcgcgcctgg cgcgcccccg gaggcttccg
    30901 ttcacggctt cttgcgctgc gagaccgcga aggggtcctg ggtgacgttg tcgatgacgt
    30961 aggcgacacc gccgacgtag cagccggagg tgccgttcgt gagggtgacg gagcccgagg
    31021 gaaggttggt cgtgagacgc gcgagcggct cctgcgcgta gcgggtcgga gggagagtga
    31081 gggcgagctt cgatccgacg gtctgaccgt cctcgtccct ggcctcgagc tcgacgacgc
    31141 actccttgtc gctcacgttg tagacgccga tgttcgtccg gaacgcggag tcctgtcgga
    31201 cgccgggaag gacgccgacc tcgcccgttc ggaggagggc cggggaggcg ctcgtgcgga
    31261 cgtcgccggc gagggcgaac tggccggtcg tcccgccggc gaagggcgcc caggtccgtg
    31321 ccccggcgac gatcgccccg gtcgaggaga tgtagagcgt cccgaaggtc tgctcgaggc
    31381 cgaaggccga cttcacgacg tcggaaaccg tcctcgagcc cttcggcgcg aggcggatcg
    31441 gggaggcttc ccggcggccc gccgcggtcc agagctcgag gccgacgagc gtttcggtcg
    31501 actcgaggtt cgtgagggag aggtcggaaa cgaaggggac gccggcggcg ttcttcgtac
    31561 tcgccaccga ggggatcagg ctgttgaatc gccaatccgg ggcgtcccgc tggaccgaga
    31621 gccgcaggat tccgcccggc gaagggagcg tggccgtctc gccggcggcc tggaaggccg
    31681 gggcctccgg cccgtccgag gtcgccacga ggttttccgc ggcgccgacg gagatgggga
    31741 gctggcgggt gatcgggctc tggccgggcg gcgtccaggt cagggcgacg gtcgtcgagg
    31801 cgaccggcgc ggtgtactgg aaggcgacgg acgggctcgt cgagccggcg ggggaggcgt
    31861 tctggccgag gtcccagctg aagcctccga tgagcaggag agagtcgttc ctctcgttgt
    31921 aggcgcgggc cgaaagcgcg tctccgacgc gaacggagat cgctccgcca cagccgccgc
    31981 cgcccggtcc gggtccgccg gggcagaggt cgactccgtc gagggcgatc cggatggacg
    32041 ggtccacgac gacggcatca cctgccacca tcaggtagta ggtcatgccc tcctcgaggt
    32101 acgaggtgag ccgcccgcga gcgcacgcgc ccgcgaccgg ggtgaggccc gcgcagccgc
    32161 cggtgtagag gccgaggacc ggctggtagt cggcgggcgc gctgcccccc gtctcgaaga
    32221 cgtacgcgtc ggaggtggcg ggctcgaact tgaaccagac cgacttcccc ggggacgcgg
    32281 tcgcgcacgg catcggaggg tcgccggcct cggtcgcctg gacggtttcg agctgctggg
    32341 tgaacgggag ctcgaggccg gagatcgtga tcgcggcggc gcaggtgtcg ttcgacggcg
    32401 gggccgcgcc ggcgaccggc gcgacgagaa gcagtgccac cagggcgagg gacgaggggt
    32461 gtctgggcat ccgggttcct cctgaagggg tgatttggag gcgaaggttc gacggtagtg
    32521 tcgctcgttc gcccgttccc acgcacgact tatgatgcgc cgatgtcccg gaccgcctgg
    32581 ggagcgatcg ctctggtcgg tgtcctctcc gcgccgcccg cccgggcgca accggtcgag
    32641 agcgcggccg aaaggccgac cgtcggcctc gtcctgtcgg ggggctcggc gcacgggctc
    32701 gcccacgtcg gcgtcctgaa agtcctccac gagctgcgga taccggtcga cgtcgtgacg
    32761 gggacgagca tgggctcgat cgtcgggggt ctctacgcgg ccgggatgtc gccgcccgag
    32821 atggaatggc tgatggggac gatcgactgg gaggacatcc tcgacgatcg cccgtcgcgc
    32881 gaccggctct cgttccggag gaaacaggac gaaatgccga gctacgtcga cctcgagatg
    32941 ggcctgacga cgcggggact cgccttcccc tcggggctca tcgccggaca gaagctggga
    33001 ttcctcctga agtccctgac gctcggaacg gtgggtatcg aggagttcga cgacctgccg
    33061 atccccttcc ggtgcatcgc gacggacatc gcgaccgggg agaaggtcgt cctcgcgggc
    33121 gggagcctcg ccgaggcgat ccgggcgagc atgtcgatcc ccggagtctt ctcacccgtc
    33181 ccgcggggcg atcgcctcct cgtcgacggt ggcctcgtgg acaacctccc ggtcggagag
    33241 gcccgggacc tgggcgccga cgtcgtcatc gccgtggacg tctcgagcga caagttcgac
    33301 atggagaagc tgaagtcgtt cggaggcgtc ctcagccgga tgacgagcct gccggtcgtg
    33361 cggaacgtct cggtgtccgc tcctcgcgcg aacgtcgtcc tgacgccgcg cgtcggcgac
    33421 atcccctcga tgggcttctc ccgcgcggcc gagatcgtcg cgcgcggcga ggaggaggcg
    33481 cgccggctcg cgaaggtcct ctcggcgctc tcggtcccgg aggaggagta ccgcgcccat
    33541 cgcgaacgga tccgcggccg aagggtcgac cggatcatcg tcgacgagat ccagaccgtg
    33601 agcgtcggcg tcgacccgag acaggtccgg gcgcgtctcg agagccggcc ggggaagccg
    33661 ctcgacgtcg ggacgctccg gagggacctc gatcgcatct acgagatggg cgtcttcgag
    33721 acggtggagt tccggatcct ccgcgacgag ggacggcacg tcctggtcat cgacgcgcgg
    33781 ccgaagtcgt gggggccgac gttcgtccgg gtcgggagcg ggttcgaggc ggacttcgac
    33841 ggggacgcga ccgtcgccct tcgcgggacg ctccacgcga tgcagctgaa ctcccgcggc
    33901 ggcgaggtga aggcgacgct cgagctgggc accgtgcccg ggctgaacgt ggagtactac
    33961 cagccgctcg acttccgggg ccggacgttc ttcagcgtgg acggctcgtt cgtccggacc
    34021 ctggcccgcg tctccacgcc ggacggcctc gtcggcgaag gacggatcac gctcctgcag
    34081 ggctccgtcg acgccgggct ctcgctcggc tcctgggggc aggtgcgcgc cggggtcttt
    34141 cgcggcgcgg gccgcgggga gcccctcgtg acgaacgtgc cgatcgacga tctcgagctt
    34201 gacgtcggcg gcctcttcgc gcaggcctcc gtcgacacgc tcgacgacct gtcgtttccg
    34261 cggcgcggga cgctggcgat cgccgccgtg gaggcgtatc gccccgacct ggggtccgag
    34321 agctccttca accggctgat cctgaggggc acccaggcca tctccgtcgg gagacggacg
    34381 gcccttcacc tgacgggcgg gtgggaggac acgatgggga gccacccgcc gttctacctc
    34441 ctcttctcgc tctccgactt cacgcgcctc gcggctctct ctcgcgagga ggcgaccggc
    34501 gagcgggccg cctacgccgc cgccgtcgtc cagaggcgca tcgcgagcct gccgacccgg
    34561 gtcgggcgcg gcgtctacct cggcggcgcc ttcgaggccg gccgcgtctg ggcccggcgc
    34621 gaggatgtgg ggttcgacgg gatccgcccc gccgggggcc tcttcgtcgg ggccgacacg
    34681 gtcctcgggc cgatttacgc cggcttcggc gtcggctacg gcggcaacac gacgctctac
    34741 gtcttcctcg gccggccgta ctgacgcgaa cggggaggac ccggtccgcc gggtcctccc
    34801 cccggcgcct tcggcgcctc cctctccgcg cgggaacgct gcgcggcgcg ccacgccgaa
    34861 acgacgaagg gccggggctt ccgccccggc ccttctcgcg aggagccggc tcagggccgg
    34921 gagcgagtca ctcggcggcc tggaagacgc cgtcgttcgt cttgttgtcg acgacgatcg
    34981 agtagacgag gaggccgcct ccctccacct cgaacgtcac gaggaagtcc tcgtgcgcgg
    35041 aatcgccggc cgtggtcggc gggatgagct cgaagaccgg ctgctgcacc tggctccagg
    35101 ccgggatcga tcggacttcc tccccgatca gcgtcgcgtt cggcgtccag gcgcggaaac
    35161 ggacgctctt cgtgtcgaag ctcgggttga agtagccgac gttcgtccgc ttgccgaggc
    35221 ccttcgcgcg gtcctcggcg ctcgcgttcg agagctggag aagggtgccg cccgcgagga
    35281 cgttctcgcc ccgggcgttg tcgaccttca ggagggtccg gccgttaccc gccgccgtct
    35341 ggtcgttgac gacggagtgg aagaacgtcg ccggaccgct cgcggtgatg cgcgcggccg
    35401 cgagcccctc gaggccgccg aagagcgagg cgaggacgtt cgggatggtg gcgtgcgtcg
    35461 cgcccgggac ggtggccgtc acggtcgccg tcggggcggc gaggcccgag ctgttcgaga
    35521 cgaatccgtc caccgtgagc gtgatctcgg cgggggacgg gttccagacg ctcaggacgg
    35581 agtagtaccg ggcgccgttg aggccttccg tattcacgac ggacggatac cagacgaccg
    35641 tcggctcgga ggcgtgggcg agcggggtgg cggagacgag ggcagcaagt gccgctgctg
    35701 cggtgacgct cttcatgttc aatctcctgt cggcggcgtt ttccggggac gcgacgacta
    35761 cgcccccgga tctcggcgcg gatcgcgtgg gacgacggag gtgtgacgcg cgtcacgcga
    35821 gccttcacag gaggcagaac gagggcggcc agcggcagga cgcgactcgg accgaaccgg
    35881 gccccgatcg gtcgctcgat gggccgtccg gccgctcggt cccgccacct cgcccgggac
    35941 ggtacgatcc cgcggcccgg gctccaccga gggaggaacc gtgaacgctc gatcgacgag
    36001 tttcgacgcc ctgatcgccg ccgccctgct cctcgcggcc gtgtcctcac ccgccgcgcc
    36061 cgtgcctctg tcgaacggcg gcttcgagca ggccgcggcc tcccgcgccg cctcgtggag
    36121 cgtcgtcggc acgctcccgc ccgcggcgat cgtcgtgcgc gacgcggagg tgaagcgcgc
    36181 gggcgccgcg agcctgcgcc tctcggcgac gagccccgcg tcgatcacgg tcgcctcgga
    36241 cgaggtccgc ctcgaggtgg gacacgtcta ccgcctctcg gcgttcgtgc ggacggagaa
    36301 cgccgtctcc gacccgacgg ggcgctaccc gacgccggtc gccgcgacgc tcgcgatgga
    36361 gtcgttcccg ttcacgaatc actccccgtc ggccggcgcg acccgaccgt tcgggaaggt
    36421 cgagacgctc ttcgtcgcga cgacggcgag ggaccgcgtc cgcctccagc tcgggcggaa
    36481 cggcgcggcg accgggaccg cctggttcga cgacgtgacg ctcgaggagg tgaccgacgt
    36541 ccgcgagctc gtcccgatgg agacggtccg ctggcacggg ccggccttcc gctacgacga
    36601 gaaggggtgg atctacgtcc acgttgaagg ggagccgtac gagcgaggct tccagtatgg
    36661 ctcgctcgtc gccgaggagc tcgccgagta cgtgaagaag ctcggcatcc aggaaaaccc
    36721 gaaggacggg ccgggcgcgt gggccgagat gcgcttcttc gccgacgccg tcttcctccg
    36781 gaagtacgac gaggagtacc tcctcgagat gaaggggatc gccgacggcg ccgcgaaggc
    36841 gggggcgaag gtcgacggga ggcccgtcga cttcctcgac gtcgtcacga tgaactcctc
    36901 gatcgacctc gactacgcga agtcggccct ccggtcgacg ccccacccgc tcaccgggcg
    36961 gagcttcctg acgtcggagg acgaggcgaa gatccccgag cgggcgcaca agtgctcctc
    37021 gttcgtcgcg acgggagagg cgacgaagga cggccgcccc gtcttcgggc agatcttcat
    37081 gtgggcgggc tacacgggcg tccacttcaa cgtcatcgcc gacgtcgtcc cgtcgaaggg
    37141 gcaccgcctc gtctaccaga cctaccccgg cgggatccac tccgggaccg actactacat
    37201 gaacgccgcg gggatcctga tcggcgagac gaccgtcgcg cagacgccct ggaacgtcga
    37261 cgggacgccg atgtcgaacc gaatccggaa ggcggctcag tacgcctcct cgatcgacga
    37321 cgtcgcccgg atccttcgcg agaagaacaa cgggatgtac acgaacgact ggccgatcgt
    37381 cgacgtgaag acgggcgagg tggccatcct cctcctcggg acgaaggagt cgaagctctg
    37441 gcggaccgga gaggacccgg cgccgttcgg gacgcccggc ttcctctggg cgaacaacaa
    37501 cgcccgcgac ccgggcgtga agagggagta cggcgtccag ccggacgacg cgccgttcga
    37561 cccggtctac ggcccgtggg accgcgacgt cgccttccgg aagtggtacg acgaggtgaa
    37621 ggggcggatc gacgcgacgg aggccgtgaa cctctgggcc tcctcgccga tcaaccgccc
    37681 gcacgcctgc gacgggaagg taacgaccgc cgagatggcc gagaagctcg tcttcctggc
    37741 ccactacggc aaggtcacgc tccgcgagaa gttccccgcg gccgggaatc gccggatgcc
    37801 ggaccacccg ggggcgatcc cgcacctgac gctcggttac gccgtcttca gccccgtgta
    37861 catcgcggag aagctgaagg aggcgaaggc gaaggagccg aaggccgcgg ccgcgccgaa
    37921 ggagccggca ctcgacgtga cggccgtcga ggagcggctc gcgtacgacg ccacgaagct
    37981 ctggaagggg agcgtcttcc cgaagaccga ggggacgaac ggcctcgtct cgggcgcggc
    38041 cgcctactgg cggctcctca aggggctgcc gaaggagccg gcgagggcgt tcgcgtccgt
    38101 tcgcgacgag ctcgccggcc tgaacgcccg agccgcctgg ttcgccgcga aggaggggga
    38161 cgtcgcgatc cgggaggcga cgcgcgagta cgggcggcgc tcgccgtacc agatcccgcg
    38221 catcaagggg accttcgcgc tccaccagct ccggctcctc ctcgggaacg agaggttccg
    38281 ggcggcgacg gacgccgcgt tcacggcgaa cgcggggcgc gaggtgacgg cggaggagct
    38341 cgtcgcgacg ctctcgaagg gagccgggcg cgacgtcgcg ccgttcctcg ccccgtggat
    38401 cgagg
//
LOCUS       DAOONL010000026        38367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_823_length_38367_cov_16.660394, whole genome shotgun sequence.
ACCESSION   DAOONL010000026 DAOONL010000000
VERSION     DAOONL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 38367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_38367_cov_16.660394"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PLP50_06135"
     CDS             <1..1211
                     /locus_tag="PLP50_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA51167.1"
                     /translation="EQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNALLVE
                     MDGFEGNDGVILMAATNRPDVLDPALLRPGRFDRRVVVDRPDVKGREGILKVHVKNIP
                     MSDDVDLSVVARGTPGFAGADLANLVNEAALAAARADKKKVEMDDFNYAKDKVLMGAE
                     RKSMVMSDKEKRNTAYHEGGHALIAAFEEHADPLHKVTIIPRGRALGVTQQLPLEDKY
                     SYNKEYVEARLAVMMGGRLAEEVCLGQITTGASNDFEQATTMARRMVCEWGMSDLGPM
                     TFGHEEGEVFLGRDFARRPDYSEDTARKIDAEVNRIVTSAYQRGKQIVIEHRAALDAI
                     AAELLEKESLDGEEVYSLIERVTGKNVPHARKVHHEGGSAGEAASHEAPSGKPSSPPD
                     PAPPAVPVPA"
     gene            1279..3867
                     /locus_tag="PLP50_06140"
     CDS             1279..3867
                     /locus_tag="PLP50_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPA51168.1"
                     /translation="MKHISERIALTALAALLVHASAKGQELPVLAEIPAIGRVFSGTL
                     STQELSRGGPPFLVMLGPDSVQVWPVGSEVEPIASPGPRHLFEPAWGDFDGDGALDLA
                     VAVPTAGVLGEGSRQIAILKGDGEGEFVAGATLSVDGGAPNAATVAALAAGDFDGDGR
                     TDLSVLSFQDSRALLLFYRGRGALEFEVPRPTDISPSAGRWLTPGDIDGDGRTDLVVS
                     SRDLIAPGVFGTTSVWRSAGDGTFAVGPTFPGPSAFPSILADLDADGRPDLVDLNRLG
                     SGAECCTYRLQVHRNQGGGSFGPWTSADVDYGRPFVADTDGDGRLDLVVPQERSSSVA
                     VFRGGPEAFTRSEIGVPLAGFEVVGADWNGDDRADFVLGTHEGVIVAGEGSSRVDVLS
                     VPVLLSTAGPGGGGFGSDLLLTNAGSTPARVFLRYVAASGGGTGTVEQVIPPSGQIHA
                     PSAIDFLREAGLAISGEGPLFGTLRISTSGASSPKALAATVRVTTQAGAGVSFGGVRR
                     LEALRETSYVPWLVETERDRSNLALVNAGGPDDGPVTLRITVESGDPATPGHAVLPDL
                     VLEPGDFRQLNRVLRVAGLATRVGWARIERVDGRAPYLAWGAVNDNASDDGSFVPATS
                     ESDRAELNMWVVPVAVQSGRYATELVVTNPGEQPVQVDVTLAATGTTLRETVAPRATL
                     HLPDFFAELRRRGLAGAPEAGVAIASPLYVGPYGATKGVLAGVRVSSVTADGRSHGLF
                     EPAVLEPHHSTSVIVPDLRQDETTRTNLAIVNLRSQSATFRLEIFDGVTGHLAASRDE
                     IPLARNELLQIDAVLREMCPGVERAWARIRAGADGSFLAYAVINDGAAPGRGTDDGSF
                     VLGLPE"
     gene            4009..4899
                     /gene="folP"
                     /locus_tag="PLP50_06145"
     CDS             4009..4899
                     /gene="folP"
                     /locus_tag="PLP50_06145"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPA51169.1"
                     /translation="MTTFRTLLGNRELRFPSPAGVMAILNVTPDSFSDGGLHASTDAA
                     VAAALRFGAAGAALLDVGGESTRPRGTAYGGGAAEVSVEEELARVLPVVAGIRAGDPA
                     VPISIDTRRTEVARKALDAGADVVNLVAGLDPPTDLLELVAERDAALVLGHMRGTPAT
                     TFEVSRFGPDVVAEVAHDLARARRRCLVAGIRPDQILLDPGLGFGKTAEQNFELLARL
                     EEIGPDGAPIVLGASRKAFLGSVSGRPAAERLPESLAACAVAIERLRDRNPVLLRVHD
                     VDETLRFVHVLGAATAAGRR"
     gene            4937..6268
                     /gene="glmM"
                     /locus_tag="PLP50_06150"
     CDS             4937..6268
                     /gene="glmM"
                     /locus_tag="PLP50_06150"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPA51170.1"
                     /translation="MRALFGTDGIRGRAGEYPLDPATVSRVGGALASVLREAGRPSPL
                     TVVLGCDTRVSSPGIVAALSGGLIAAGAGVRFAGVVPTPAVAHLVRALGADAGISISA
                     SHNPWPDNGIKVFSSEGRKLPDALEAEIERRIATSAPAEPIPPAEDSSLPELYENHLV
                     ETLPKRLDGLKVVVDCANGAAFQVAPEAFRRAGAEVVPLAAAPDGRNINEGCGALHPE
                     AMANAVVRSGAHLGLALDGDADRAILADGEGRLLDGDDVLLLWALELEREGKKPGSVV
                     GTVMSNWGLEEALAARGIRLVRAAVGDRYVVEEMERHSALLGGEQSGHLIRADLTTTG
                     DGTLTGLHVAAVVAASGIPLAAGARFTRAPQVLRNVKVREKVPFERVPGFPHLVAGAE
                     SRIAGSGRILVRYSGTELLARVMVEGTERGTVESIADELAGALQGALGASD"
     gene            6288..8603
                     /gene="ppk1"
                     /locus_tag="PLP50_06155"
     CDS             6288..8603
                     /gene="ppk1"
                     /locus_tag="PLP50_06155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPA51171.1"
                     /translation="MIRHEQRPRRKRSPEAIEEPAPTLLEPPDEPAPAEPLAEPPGAV
                     EERGEVRSPVPLLNRELSWLDFNQRVLEEARDERWPLLERLKFLCISESNLDEFFMIR
                     VSGLRDQLSADLAERSDDGLTAREQLALVRKGVLAMEAEQTACLHEDLLPRLAAAGIT
                     LLSWGDLEEERREAARGYFRRNVLPVLTPLAVDPGHPFPFLSNLSVNLAIETRNPENG
                     EVKFARVKMPPAIPRLLPLRTVVEGKKKVKAARAEFLLLEDLVQANLDELFPGLEILA
                     SYLFRITRDADIEIQEDEASDLLATIEQEVRRRRFGAVVRVEVTPQTPKKMRKLLMRQ
                     LEVSESDIYEVSGILGAADLMALVRLDRRDLKDAPFTPALPAALASTEGSIFQAIRQG
                     DLLLHHPYDSFGPVVELIEEAADDPKTLAIKMTLYRTSSDSPVIPALVRAAEAGKQVA
                     VLVELKARFDEENNIVWAKALERAGIHVVYGVVGLKTHAKIALVVRREKDEIRRYVHL
                     GTGNYNPATAKVYTDLGLLTCRPEFGEDATRLFNTLTGLATRTSYNRIVTAPRDLHRA
                     VLEWISRETEHARAGRPARIRAKLNALVDTAVIEALYEASRAGVPVDLIVRGTCCLVP
                     GLPGVSETVRVRSIVGRFLEHSRFFLFENGGDPEVWASSADWMPRNFFRRVETAFPIV
                     DPELGRQLSDAFDAMMADNARARALRSDGTYERLRPGRGEPAIDSQAFFLDQARRRVL
                     KAAEHFVKGAAADGFDRVPDPRERGPEDEDR"
     gene            complement(8628..8843)
                     /locus_tag="PLP50_06160"
     CDS             complement(8628..8843)
                     /locus_tag="PLP50_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51172.1"
                     /translation="MDIPAKVYITCPSAELKKQPGTLIAVSANGYYEVHVQFGSNTHA
                     MLLPITETALLNAEPVLTPPAGFELER"
     gene            complement(8879..10336)
                     /locus_tag="PLP50_06165"
     CDS             complement(8879..10336)
                     /locus_tag="PLP50_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA51173.1"
                     /translation="MSAAPPREPLVLVVDDDAGSRKAMALTLTTDGRRVEEFPDADSA
                     LSRAMDDPSVGLVVTDLKMPGKTGIELATELAAARPDVSVLLVTAFGDIETLLKAKDL
                     GTVDYVAKPVARDDLRLRASAALGRARQAGEIKQLRERLDKRYGFEAIIGVSAAMERV
                     FDVLRKVAPTRMNVLITGESGTGKELVANALHHNSPRRPRAFVALNCGAIPKEIIESE
                     LFGHEKGAFTGALVKRMGRIEQAHGGTLFLDEVSEMPPDLQVKFLRVLEERRVTPVGG
                     NDSKEVDFRLVSATNRDLKKEIEAGRFRQDLYYRLKGVVVDLPSLRERREDVPLLAER
                     FREVFAREHERAVTGFTPAALSALMSYDWPGNVREMKSAIEWAVFSAAGPRIDVGDLP
                     PDVKGDSEGEGPHPEGTVPIAHSAILVGKTMAEIEKEAILTALQASGGNRRKAAERLE
                     IGLRTLQRKLKEYRGETGGDEGEDEADDDGDGPTA"
     gene            complement(10333..12360)
                     /gene="ligA"
                     /locus_tag="PLP50_06170"
     CDS             complement(10333..12360)
                     /gene="ligA"
                     /locus_tag="PLP50_06170"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPA51174.1"
                     /translation="MLKLPETVAAATERAAFLRAELRRHEHLYYALARPEVSDAEYDA
                     LARELLAIEERWPDLVTPDSPTRRVGGTPVDDLPNVRHEVPLLSLENAYSEAELDAWL
                     TRVTDRLDGRLPEVVCELKIDGLSISLVYEDGVLVRGATRGDGTTGEDVTPNVRTIRV
                     LPLRLPEGAPRLLEVRGEVYLGKAAFRALNAAREEAGEPLFANPRNAAAGSLRLLDSR
                     ETARRRLSVYLYSVARWEGDGGPATQVEALDALARLGLPVNPHRAVARSAGEVKAFLA
                     EWREKRHALDLETDGAVLKVSPVSDQQRLGATTKAPRWALAYKFPAEEASTRVTRIVV
                     QVGRTGVLTPVAEFEPVLLAGTTVRRATLHNYEDLSRKDVRVGDTVAVEKAGDVIPKV
                     TRVLLEKRPEGAVSFEMPAACPECGEAVVREEGEVAVRCVSGSCPAQVKEAIRHYVAR
                     RAMDVEGLGDERVDQLLAAGLIADVAGLYALRAEDLAALERWGGKSAANVLGEIERSK
                     EAGLGRLLFGLGIRHVGEKTGKVLARRFGTMEALSAATEEELTAVPEIGPETARSVRD
                     WFTLPANVRLLSKLAEAGVRMNEPEGAPAPGGPLSGMTFVLTGTLPRRTREEATAAIE
                     RAGGKVSGSVSRKTAAVVAGDEAGSKLEKARALGVPVWSEDDLDRAIGGGR"
     gene            12442..12849
                     /locus_tag="PLP50_06175"
     CDS             12442..12849
                     /locus_tag="PLP50_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HPA51175.1"
                     /translation="MKRKLLLAGGMVALGLGLAGLLLPVLPTTPFLLLAAALFARSSP
                     ERYERLLGHERLGPHVRDWVERRGLRRSVKVRAILLLWAALLPSSLWLGHRHLSGFLL
                     LAVGAAVSAYLLRLPTLEEPPADAALPKSGDAT"
     gene            12860..14836
                     /locus_tag="PLP50_06180"
     CDS             12860..14836
                     /locus_tag="PLP50_06180"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPA51176.1"
                     /translation="MSSPKAFLGLVLLVAGLPARAGERVPPVASYRIEASWDEERKVL
                     AGRETITFVNRTTRPFDDVVLHLYLNGFRNTRSTLYRALPRPTGSDGFGYCELKRIAP
                     SGGEDLTRRVTFASGGADNPDDRTLVRVPLTRPVAPGETLVLEVDFESRLPRGLLRTG
                     WKDDFVFAGQWFPKLAKATEAGWRGRPFHPFTEFFADFGDYDVTLLLPAEVKGRVAAT
                     GRVVEESDADAGRVRVRARAEDVHDFAFAFSPRFEVKTEKVAPKGLPAVEVRLYLQPD
                     HRRSRERYLRAAREGLAHYGSWLGPYPYPVLTIVDPPTGSGTGAMEYPTLLTGGTTWL
                     SPAAGGEPGAVVLHELAHQWFQGVVATDEVSEAHLDEGLATWAAARAVTRLQGPPSPV
                     VEAFGIPIALRSLSRTLPEGEAQDALDQAAFSRSDPTLRESWRALDDAAVRTNAYSRT
                     ALLLESVARTIGDQPLRAAVGEYVRRFAFRHPTTTDFLDVVGEVAGAEARALLERGWA
                     SAGPADYAVTKAETARMRPPAGFLGEGPRRAWAAPTQGRGEWASTVVVQRLGEVAWPV
                     VVELRFEGGHVVKRTWDGRGEWIRYRATGPRLLSAVVDPGRACLLDVNRLNDGLATER
                     DPRAARAWGHRLRFLAQNVLELFALVAALPGAAR"
     gene            14833..15804
                     /locus_tag="PLP50_06185"
     CDS             14833..15804
                     /locus_tag="PLP50_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51177.1"
                     /translation="MKGDALPVLWARGLLSAARTWKALLLALALNAALALVVTAPLAS
                     RLHDLLDERPTAAPLANGRDLVLEAHLLRAESGLLGGRARLEALGRGELLLEELPLLS
                     GPLAGLLTAGLLHAALSGLLAGGFAGRFASERDRGSLAAFLSDVVRLGLPSLVLGLLG
                     LAGILGAFWVFVELPRAATLSPDLRWDQERTGLSLLRLGAFLLAAGAIRAVVAQSRAA
                     LGLSRSVNPLAALGAGLGFVLGRPVKALLLEVAFGATALLPLALWLLFAPSLGGGDLR
                     LLATLVAGQQVVVLLRIASRTAHLGAASAWMRGAREASRPAPAKIES"
     gene            15801..16391
                     /gene="miaE"
                     /locus_tag="PLP50_06190"
     CDS             15801..16391
                     /gene="miaE"
                     /locus_tag="PLP50_06190"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HPA51178.1"
                     /translation="MIPLRTKTPGSWAERAAARLPELVADHAACELQAAVFALALVGR
                     YPGDPLLVDRLSALAAEELRHFRKAMREARRLGARVGTRRANAYVSVLRAAGRAGREP
                     EQGLDLLLCGALVEARSHERFLALVPRLAAEPRLAKLYVELAAAEERHGPAYLELAER
                     HAGREATAARLEELLDVEARAIAGAPTSPVAVHSGA"
     gene            complement(16405..16800)
                     /locus_tag="PLP50_06195"
     CDS             complement(16405..16800)
                     /locus_tag="PLP50_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPA51179.1"
                     /translation="MDGKPLGEAEIIARKLERAGVDVARRHVFLCADRENPKCADAER
                     AMAAWTHLKDGLKRRGLSERGGVLRTRVGCLRVCERGPIAVIYPEGIWYRACDPPVLD
                     RIMDEHLVGGRPVEEFVIARHSLPGGDGD"
     gene            16935..17855
                     /locus_tag="PLP50_06200"
     CDS             16935..17855
                     /locus_tag="PLP50_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51180.1"
                     /translation="MGLLKDFLRIDADRRSALATVRQEAQSHRLRIEELGAEARRKRD
                     EIGMLEEELRRLAEDVARTEELLLDLEARREAHDRDTHERKVDAVRSGLAHDRADGFR
                     IAEEFRQLRGTFEGERTRLLAEADTGRMMDNFFQIEAFLKDTGTPIPDAARKALMKER
                     QDLMARIGPLVAPPPVPDGVFKATVVYSTLEEGEPTAVVAVGLPDEADVSGPHDLSAL
                     LLYASYASVVEKIGPGVPRPRRSDGVVVYEQPAGSRPADEVALDLFLAVEDGLRKGAA
                     AAGVPCELTGVFLEPEIASAVFPRGDARGI"
     gene            17868..18302
                     /locus_tag="PLP50_06205"
     CDS             17868..18302
                     /locus_tag="PLP50_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51181.1"
                     /translation="MTVKWIGDAAQELGLSPLDAVEILASRQEYPMNGLLDEDRFKTL
                     RLMRGSKRLETSGSQPMPVPDRLASPSTVPIPTPPPQAHAAPPPAPVVTAPPPVPEPF
                     APAPPPVRPPVGERTSVTAPLQTIPGREGEKTVILSPIEPKE"
     gene            complement(18339..19511)
                     /locus_tag="PLP50_06210"
     CDS             complement(18339..19511)
                     /locus_tag="PLP50_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51182.1"
                     /translation="MDADVVVVGAGVVGLATAAELSRAGLSVVVAERHPGPGRETSSR
                     NSQVLHAGLYYPPGSRKARLCVAGNRNLAAFCEAHGVPFRRTGKWLLALEDGEEERLA
                     QIVTAGRENGALLEEVSLSRFREEEPHVRATAAVLSPSTGILDVHGLFRVFRAVAEEN
                     GALFAFRHVLKAAAPARGGYELVFVDPSGEEVRLSTPRVVNAAGLDADVVAAMPGLDV
                     DAAGYRQTWTKGSYFRVRSSRRHLARRLLYPVMPHGYGSVLGIHLTVDLDGELKLGPD
                     VEPLEERRADYAVDESRRGAFLAAARRYLPALVADDLSPDQSGIRARLEVIGAPFRDF
                     VVAEESERGLPGWVNLVGIESPGLTCCLELARQVRELLVPASGGRAVAARSAASRS"
     gene            19598..20854
                     /locus_tag="PLP50_06215"
     CDS             19598..20854
                     /locus_tag="PLP50_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51183.1"
                     /translation="MSPTPPDAPGAVTARLAARGLAILTLVNLFNYLDRFVVASLVES
                     LRNSELRLTDTQAGALMTGFIVVYMVASPFFGVLGDRGSRPRLLALGVFVWSLATAAA
                     GLARSFASLFAARAAVGIGEAAYGTIAPSLLADYFPREKRGRVYAIFFCAIPIGSALG
                     YVVGGLADTHLGWRAAFYVAGLPGLLLALLALTLPDPPRGVTDAAEAPPPGTPKDSGW
                     SSYLALAKNRPFRLAVLGYAAYTFGLGAMAFWMPAFLERVRGVPKETATIRFGLVVVV
                     TGFVGTFAGGFLGDALLKRTKEAYLWVSGVATLLAVPFAVVALTAAAPGVYFPALVVA
                     QLLVFASTGPINSAIVNDVLPWQRAAAVAACNFTIHVLGDVPSPPLLGAVSDASTLAN
                     AVLLVPAAFLVGGAIWTWGALTGRRA"
     gene            20851..22161
                     /locus_tag="PLP50_06220"
     CDS             20851..22161
                     /locus_tag="PLP50_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPA51184.1"
                     /translation="MIRTLATAVSRLVLRVFYRNVEAAGLDRVPAAGPTILVLNHPNG
                     LMDPLLLLCLSPRPVSFLAKSPLFTMPFVSLFVKAFDSIPVYRPQDADADVRRNRETF
                     AKAREMLKRGGVLALFPEGVSHDEPRLMPLKSGAARIALGAAAEGAVTVVPAGLTYEA
                     RDLFRSSALLHVAEPFTVEPIPLDEKGEPPRTAVKALTERLREALEGATLQADDAATL
                     ELAAFAESLLPAGDGSLEARVAVRRLLLERAALLRDRAPERLAALVARLERFRAILTA
                     ARLAPGALDSGASRAELAASTLAALLVALFLAPPALLGLLAHAPAYNLVGPLAKRLSR
                     GNTDIVATIKVLGAFLFFPLTWIALGTAAGLAWGLPLGLLTGLALPVLGYLALLLLER
                     WEATVAALRALGLALVRRPALARLRAERESLVAEMAALDAECRV"
     gene            complement(22215..23186)
                     /locus_tag="PLP50_06225"
     CDS             complement(22215..23186)
                     /locus_tag="PLP50_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPA51185.1"
                     /translation="MLPSFRYVRPRTVAEAVKALAAEGARAHAGGTDLLGCLRDGVFD
                     AKSVVSLSGLADLKGISATKDGGLRIGALTTLAEVARHAGIAKGWPALAQGAAAAASP
                     QLRNQGTLGGNLCQRPRCWYFRGDFDCARKGGEICYAVEGENTYHAVFGGSGCYIVHP
                     SDTASPLVALRATVKVVGPKGVRTLPVEKLFVLPEVDHTRETVLAPGEVLTEVLLPPP
                     LEGGKGHYRKVRSRGAWDFALGGVALALSVKDGKVASAGVVLSGVAPTPWRVPEVEKL
                     LVGKALDAKLAAEAAELAVKGAEPMSGNDYKVPLVKGAVEEALLALA"
     gene            complement(23191..25488)
                     /locus_tag="PLP50_06230"
     CDS             complement(23191..25488)
                     /locus_tag="PLP50_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPA51186.1"
                     /translation="MTDLLYWLQGPVPETEPPKAEMPPWGETKVVGKPVPRVDGYERV
                     SGSAVYPLDFALPDMLHAAILRSPHAHARVKAIDTSAAEKMPGVRAVLTAFSPGADVP
                     WYRSRGRFYSKLFDERVRHEGDEVAAVAAETRAQAFDALKAIRVEYEEFAFVLDPREA
                     LAPDAPLVHDGGNRYGEPARYERGDLAAGFAAADVVVEGTYSTACEIHAPMEVHGSVA
                     KWDGNRLTVWDSTQGVFVVQQTVAAALKLPLANVRVIGHYMGGGFGSKLEAGKYTVIA
                     ALLAKRTARPVKLFLTREEVFLNAGNRPANTMKVKIGAKKDGTLTAIEFSSIGSGGAY
                     PNGADTSFLATDLYTCPNVAAVDENVAINAGRARAMRAPGFPQGAWALEQAMDELALK
                     LGMDPVALRLANVPKVSQRRGNLPYTSTGLAECLSGGAKAFGWETTREKRKDDGPIRK
                     GYGVAAGMWGYGGGPPATVVVKLYADGSVNVNSGAADIGTGTKTVMAQTVAEELGVPL
                     EKIQIEHADTGTTQYSGPSGGSKTVPSDMPAARAAAIEVRTKLLAGAAEELKVPVAEL
                     KLDGNDVVSTKDPAKKLAVTAVPALQRNQVLVGVGTRGPNVEGKVISMFAAHFAEVEV
                     NVRTGEVAVTKLVAAQDSARVMNRLTFECQVQGGLVMGLGLGRTEERVLDRQTGKMVN
                     ANWHDYKIPTALDVPAAQEVLSADLVDTECNTAGAKGVGEPATIPTAAAIANAVRDAV
                     GLRVHDSPITPPKLAALLAAAGKKG"
     gene            complement(25493..26134)
                     /locus_tag="PLP50_06235"
     CDS             complement(25493..26134)
                     /locus_tag="PLP50_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPA51187.1"
                     /translation="MAERSDDRNDLDTNGGVSRRGFLGSVVPAAVGVAVAKEALGAPA
                     AAPAAIAELPAAEPTKLTLVVNGRTHKVLVEPRDTLLSVLREKLGLTGTKPGCERGEC
                     GACTVLIDGVARYSCLTLALEADGKKVTTVEGLMEGEELGPVQKAFVEEDAYQCGYCT
                     PGQVMAAEGLLRENPEPGLDEIRHGMSGNLCRCGAYAHIFKAVDKAARLRKER"
     gene            complement(26322..28118)
                     /locus_tag="PLP50_06240"
     CDS             complement(26322..28118)
                     /locus_tag="PLP50_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HPA51188.1"
                     /translation="MTGGGALAQLQTIGRALMLPVSVLPVAGLLLGLGNARLGLLPAG
                     AAHLLAEAGGAVFAALPLVFAIAVALGLSGNDGAAAVAAVVGYVVFVATLGAGANALG
                     LPTVRVLGFESVETGVFGGILVGLVAAALFRRFSRSDLPAALGFFGGPRLVPILTGLA
                     ATLLGLALSVVWPPLSDAVRLLSDRALEAGPAAAVFSYGVVDRLLLPFGLHHIWNVPF
                     FFELGTYVDRAGTAIHGEIPRFFAGDPRAGILGGGFLFKMWGLPAAALAIWRSAREDR
                     RARVGAVMLSAAATSFLTGVTEPIEFAFLFLAPFLYALHALLAGLSYLVAELAGMKLG
                     FTFSHGLFDLVLYWPMGTRPWLVPLLGPLWALVYYALFRAAIVRLDLRTPGREEPATP
                     AARPESPTPAAEPGRSEGIAERLVAAFGGARNIESLDACITRLRVTVQDLDRVDEGEL
                     GALGAAGVVVLGNAVQAVYGTRSENLKAAMEGVLGGRAGPAPREPSGPHGAVSPDVAA
                     RGRRLVEALGGAGNVRTVEACARTRVRVVLADPARVDEAGVRAAGADGVMRLPGGVVH
                     VLAGDEAAELAVALGRDAELSGAVEHRARIGE"
     gene            complement(28115..30655)
                     /gene="ptsP"
                     /locus_tag="PLP50_06245"
     CDS             complement(28115..30655)
                     /gene="ptsP"
                     /locus_tag="PLP50_06245"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPA51189.1"
                     /translation="MNEPPTGGPRAASPEPTLALKAPLSGPVVPIERVPDPVFAHRLL
                     GDGVAIDPVSAILLSPCDGVVATVHPSGHAVAIAADAGVEVLLHVGLDTVALGGRGFV
                     PRVAQGRRVVAGEPLLAFDADLVARSARSLVTPVVVTSRERVTSARVAGGPVVAGVDD
                     LMVLTLVPSASALVAPAAGERTRDVRVMRRLGLHARPAAALASAARRFRAEVLVETGT
                     SMASARSIVGLLSLGVVHGEVVRLRATGPDAGKALSALARVLEAEEGHEAAAPLPSAP
                     RPVRTGARVFPGIPSAPGCAAGPVVRLAREEPEVVEEGSDGRTERRDLDFALEEAKVQ
                     LRALEARLGAEAGSEHADIFEAHVALLEDPLLLSEAEHEIASGKSAAFAFREAVRRYS
                     DRIASLPNPALAARAADLRDAGGRVLRILAGSRSSAPAPPEGAIVVSEDLTPSEAATL
                     GRGGVAGFCTVAGGATSHVALLARSMGIPAVAGIDARALEVEPGVPGVLDGDAGELRL
                     APDEAEREAVRERCARRAARRTADRLAALEPARTRDGVRVTVLGNAGSLADAEDAIAA
                     GAEGIGLLRSELLFLDRANVPTEDEQEAVYRAVAEAVGPGRPLVVRTFDVGGDKPLRW
                     LPLPEEENPALGERGLRVFIDRPDLFRAQLRALLRVPPEYDVRVTFPMVATLDEWRGA
                     LADVEEERAALGAPPRVAGMTVEVASAAILADAFLREAGFATIGTNDLTQFTLAMDRG
                     HPRLSPRADALDPAVLHLVARTAAAARKRGVPVAVCGALAGDLAAVPLLLGLGVGELS
                     VGAAQVPSVKARVRELSLEECRSLAVGALEAGTAAEVRALLAASWGAA"
     gene            complement(30701..31297)
                     /locus_tag="PLP50_06250"
     CDS             complement(30701..31297)
                     /locus_tag="PLP50_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPA51190.1"
                     /translation="MPGRANPILSSRFDEALAFAVELHREQPRKGTGVPYVSHLLSVA
                     ALVLEHGGSEDQAIAALLHDAVEDQGGRPTAERIRERFGDLVADIVDGCTDTDVSPKP
                     PWRLRKEAYVARVRNEPAHVRLVSAADKLHNARTMVTDLRIHGPALWERFNAGRDETL
                     WYLESLVAAFREAGSTPIVEELARTVAELRLVSGNGHG"
     gene            31374..32159
                     /locus_tag="PLP50_06255"
     CDS             31374..32159
                     /locus_tag="PLP50_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51191.1"
                     /translation="MSPARKAAPKRPRPSAAPVPSAPADASGLGDVPPPLVYERTAAA
                     AEDEGFDAATAAVFAAVAAVDVFCVSETSGSVRHRLGSLVHAADLAEHALEDVGAGEE
                     EFTHLFAPRLPFDVLLGETGRLTAADLFLERWGDELPKDAVRAVKALVRAEDTAARAT
                     WAGKSATILDLATGRALPGPALLRRGTRPFVCRLVRLGSAFVPLAVERIVNRVTEEEL
                     LAEVRGAAQLGRDVLKAAGLRLRTTKKGFGLGARMLAAADEMG"
     gene            32326..34413
                     /locus_tag="PLP50_06260"
     CDS             32326..34413
                     /locus_tag="PLP50_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPA51192.1"
                     /translation="MREPWAGESQTSRHRLLSELIEASRALRGVGGIALSPFAFQGVF
                     VVLAGFGWPRAGAEHRLLRDAPVPFLVSSLLVSALASAALVLACAGVSALSGRKTRRA
                     GIFFVALLASLPIPMLAFFVGSGTLPSLDLAREGLPFLLRDATGWGRAAAVTLACVAV
                     PWALLSVLTLRSASAPPRGSFAPLPLASAGLLLLGAVFLLFAGGNVLAHVHPGSRRAA
                     DSRRQLLTCLFPSFPLLPALGADEVGAEALVDRLGRELPPRSELARYLSSLASLEAAP
                     RPNVVVVMLEGVPAGHVGFRGYRRSTTPNLDRLAAAGVSFDRAWAAANESKNGQTAIL
                     ASVYPMRGAENNFVSNALDYPRILCWEVFRALGYATAFISTQDETWLGMQRFQLLDGR
                     PPDLYVHALDIPEGETFPDRKKDEAPVVRRALDWMRRVADPRKPFLLVTNFQRTHFPY
                     QLPSGIPPSFQPLAPLGPRFRRWTEADLPNGVNNFDSALRYVDDRLGELLDGLRDLGV
                     EKDTIVAIVSDHGQGFEAGLEPVSMSLDAPYVHVPLVLSWPGTLPAGRIEENVSAIDL
                     FPTLLGLIGAPPCPGWDGRARLGGPASSRGRSRPIYSASIVWEARWLVQGAQEKVLVD
                     LMTGRATAFRGDASLRSPTDRGSFDEAPALLRSFLEDYSRRATYYTSPDLRRSRLYAE
                     PADSDGGALSRTD"
     gene            complement(34502..34822)
                     /locus_tag="PLP50_06265"
                     /pseudo
     CDS             complement(34502..34822)
                     /locus_tag="PLP50_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012346.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
     gene            complement(34849..35532)
                     /locus_tag="PLP50_06270"
     CDS             complement(34849..35532)
                     /locus_tag="PLP50_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPA51193.1"
                     /translation="MKVVLFGATGMVGQGVLREALRAPDVELVLAVGRSPTGLTHPRL
                     RELVHRDFTDFSGVEAELGGFDACFYCLGVSAAGSSEADYRRVTYDFTLAAANALARR
                     NPALTFVYVSGAGTDATAKGSGMWARVKGETENALRRLPFRGAYMFRPAFIRPAHGER
                     SKTASYRAFYAATGWLFPLLRRVAPRYVTTTEEVARAMLHVAREGAPQAVLENEDIVR
                     LAGSTNRSS"
     gene            complement(35606..35893)
                     /locus_tag="PLP50_06275"
                     /pseudo
     CDS             complement(35606..35893)
                     /locus_tag="PLP50_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            36109..36729
                     /locus_tag="PLP50_06280"
     CDS             36109..36729
                     /locus_tag="PLP50_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA51194.1"
                     /translation="MTTSPDSGARRTGILEAAAKVFLRYGYRKTSMDDLARAAGLSRQ
                     GLYLHFETKEALFQAAILGVIEGARAAYLSALAREELPLFDRVLGAFEAFHGCHIGQL
                     GEQYVGEIIEAAASLLGDAPAEHERRFVADVTEALAGDPGLGRTGPGIGPRELAETLC
                     ATSYGLKHRVATPAEYRDRMAVALRIALPGVPPGRRDAASARPRKR"
     gene            36734..37843
                     /locus_tag="PLP50_06285"
     CDS             36734..37843
                     /locus_tag="PLP50_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPA51195.1"
                     /translation="MKTVLAVGIPVAVLALLAASIWYVAGRLRTLFGLERRWPLRLGV
                     AAAVVGAAVAMLGTARLAHPLAGVVNLLGGYAFLFFVFLLLALLGLHAIQLRWTRPPA
                     WSGVAAVSLAVAATGAGALRAGSFTVAETVIRLPRLAREVTVMQISDVHLGHHRGRAY
                     LARIVDETNRRAPDLVLVTGDLVDSSAALEPGVLEPLAGLTAPAYFVGGNHEKYVDPE
                     RVFALVARNGVRVLHNEAVETHGLRLVGLDYMNADEDAFDMHPSDDARTIRSVLPGLA
                     SRNGLPSVLMHHSPVGASYAAEQGIDLMVSGHTHAGQVFPFTLLSGLAFPFNRGLHYE
                     GATAVFVSQGAGTFMSRARLGTSNEINLLRLVPGA"
     gene            complement(38192..>38367)
                     /gene="groEL"
                     /locus_tag="PLP50_06290"
     CDS             complement(38192..>38367)
                     /gene="groEL"
                     /locus_tag="PLP50_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPA51196.1"
                     /translation="DLLKAGIIDPAKVTKSALQNASSIASLMLTTEAMISEIKEKEKA
                     PAMPPGGGMGGMY"
BASE COUNT         4896 a        14672 c        13441 g         5358 t
ORIGIN      
        1 tcgagcaggg gaagaagaac gccccctgca tcatcttcat cgacgagatc gacgcggtcg
       61 gtcgccaccg cggcgccggc ctcggcggcg ggcacgacga gcgcgagcag acgctgaacg
      121 ccctcctcgt cgagatggac ggcttcgagg ggaacgacgg cgtcatcctg atggccgcca
      181 cgaaccgccc cgacgtgctc gacccggcgc tcctccgccc cggccgtttc gaccggcggg
      241 tcgtcgtcga ccgccccgac gtgaaggggc gcgaggggat cctgaaggtc cacgtcaaga
      301 acatcccgat gtccgacgac gtcgacctct ccgtcgtggc gcgcggcacg cccggcttcg
      361 ccggcgccga cctcgcgaac ctcgtgaacg aggcggcgct cgccgcggcg cgcgccgaca
      421 agaagaaggt cgagatggac gacttcaact acgccaagga caaggtcctg atgggggcgg
      481 agcggaagtc gatggtcatg tccgacaagg agaagcggaa cacggcgtac cacgaggggg
      541 gccacgccct catcgcggcg ttcgaggagc acgccgaccc tctccacaag gtgacgatca
      601 tcccgcgagg gcgcgccctc ggcgtcacgc agcagctccc gctcgaggac aagtactcct
      661 acaacaagga gtacgtcgag gcgcgcctcg ccgtcatgat gggcgggcgc ctcgccgagg
      721 aggtctgcct cggccagatc acgacgggcg cctcgaacga cttcgagcag gcgacgacga
      781 tggctcggcg gatggtctgt gaatggggaa tgagcgacct cgggccgatg acgttcgggc
      841 acgaggaggg cgaggtcttc ctcgggcgcg acttcgcgcg gcgccccgac tactcggagg
      901 acacggcccg gaagatcgac gccgaggtga accggatcgt cacgagcgcc taccagcgcg
      961 gcaagcagat cgtcatcgag caccgagccg ccctcgacgc catcgccgcc gagctcctgg
     1021 agaaggagtc gctcgacggc gaggaggtct actccctcat cgagcgcgtg acggggaaga
     1081 acgtcccgca cgcgcggaag gtccaccacg agggaggctc cgcgggcgag gcggcgtcgc
     1141 acgaggcccc ctccggaaag ccgtcgagcc ctcccgaccc ggcgcccccg gccgttcccg
     1201 tcccggcctg acggaccgcg ctccgggggc gtccccgccc gggccgtggg ttcgcaccgg
     1261 cccgggacac tacctgccat gaaacacatt tctgagcgga tcgcgctcac cgcgctcgct
     1321 gcgctcctcg ttcacgcttc cgcgaaggga caggaacttc ccgtcctggc cgagatcccc
     1381 gcgatcgggc gggtcttctc ggggaccctt tccacgcagg agctctctcg cggcgggcca
     1441 ccgttcctcg tgatgctcgg gccggactcc gtgcaggttt ggcctgtcgg cagcgaggtg
     1501 gaaccgatcg cctcgccggg accccggcat ctcttcgagc cggcgtgggg cgacttcgac
     1561 ggcgacggcg ccctcgacct cgccgtcgcc gtccccaccg cgggcgtgct cggagagggg
     1621 tcgcggcaga tcgcgatcct gaagggggac ggcgaggggg agttcgtcgc gggcgcgacg
     1681 ctgagcgtgg acggcggagc cccgaacgcc gcgaccgtcg ccgctctcgc cgccggcgac
     1741 ttcgacggcg acgggaggac cgacctgagc gtcctctcct ttcaggactc gcgggcactc
     1801 ctcctcttct accgcggccg gggcgcgctg gagttcgagg tcccccgacc gacggacatc
     1861 agtccgagcg ccggccggtg gttgaccccg ggagacatcg acggcgacgg gcgaacggac
     1921 ctcgtcgtct cctcgaggga tctgatcgcg cccggggtgt tcggaaccac gtcggtctgg
     1981 cggagcgctg gcgacggcac gttcgccgtt gggccgacct tcccgggtcc gagcgcgttc
     2041 ccctccatcc tcgcggacct cgacgccgac ggacgacccg atctcgtcga cctcaaccgc
     2101 ctgggatcgg gcgcggaatg ctgcacctat cgcctgcagg tccaccggaa ccagggcggc
     2161 gggagcttcg gcccctggac gagcgcggac gtcgactacg ggcgcccgtt cgtcgcggac
     2221 accgacgggg acggccggct ggacctcgtc gttccgcagg agaggagctc gtccgtcgcc
     2281 gtcttccggg gcggtcccga agccttcacg cgaagcgaga taggggtacc cttggccggc
     2341 ttcgaggtcg tgggcgccga ctggaacggc gacgaccggg ccgacttcgt cctcgggacg
     2401 cacgagggcg tcatcgtggc gggagagggc tcgtcgcgcg tcgacgtcct ctccgttccc
     2461 gtcctcctct ccacggcggg gccgggaggc ggcggcttcg ggtccgacct cctcctgacg
     2521 aacgcgggga gcacgccagc gcgggtgttt ctccggtacg tcgccgcgag cggaggaggg
     2581 accgggaccg tcgagcaggt gatcccgcct tccggccaga ttcatgcccc ctcggcgatc
     2641 gacttcctgc gggaggccgg cctggcgatc tcgggggaag gaccgctctt cgggacgctg
     2701 cgcatctcga cctcgggcgc ctcctcgccg aaggcgctcg ccgcgacggt ccgcgtcacg
     2761 acgcaggccg gcgcgggggt ctcgttcggc ggcgtccggc ggctcgaggc gctccgcgag
     2821 acgtcgtacg tgccgtggct cgtcgagacc gaacgggacc ggagcaacct cgccctcgtg
     2881 aacgccggag gtcccgacga cggaccggtc acgctgcgga tcacggtcga gtcgggagac
     2941 ccggccacgc cgggacacgc cgtccttccg gacctcgttc tcgagcccgg agacttccgg
     3001 cagctgaacc gcgtcctccg ggtcgccggg ctcgcgaccc gcgtcggctg ggcccggatc
     3061 gagcgggtcg acgggagggc cccgtacctg gcctggggag cggtgaacga caacgcgagc
     3121 gacgacgggt cgttcgtgcc ggcgacctcc gagagtgacc gggccgagct gaacatgtgg
     3181 gtcgtaccgg tcgccgtcca gagcggccgc tacgcgacgg agctggtcgt gacgaacccg
     3241 ggcgagcagc ccgttcaggt cgacgtcacg ctggcggcga cggggacgac cctccgggag
     3301 acggtcgcgc cccgggcgac gctccacctg ccggacttct tcgcggagct gcggcgtcgc
     3361 ggtctggcgg gcgccccgga ggccggcgtc gcgatcgcca gcccgctcta cgtcgggccc
     3421 tacggcgcga cgaagggagt cctcgcgggc gtccgtgtct cgagcgtgac cgcggacggc
     3481 cgctcgcacg ggctcttcga gcccgccgtc ctcgagccgc accactcgac gtccgtcatc
     3541 gtgcccgacc tccgacagga cgagacgacc cggaccaacc tcgccatcgt gaacctccgc
     3601 agtcagagcg cgaccttccg cctcgagatc ttcgacggcg tcacgggaca cctggcggct
     3661 tcgcgcgacg agatccctct cgcgaggaac gagctcctgc agatcgacgc ggtccttcgc
     3721 gagatgtgcc ccggagtcga acgcgcgtgg gcacggatcc gcgcgggggc cgacggcagc
     3781 ttcctcgcct acgccgtgat caacgacggc gccgcgccgg gccgaggcac ggacgacggc
     3841 tccttcgtcc tgggcctgcc ggagtgacgc gaggaccggg acgcctcgcg tccggccgcc
     3901 gcgctccgtc ttgcgcggcg gctgctcctc ggcggcgccc tccgccgccc cgggcccggc
     3961 attcgtggga gcgggcgagg tcctagactc ccggccggga cgctcgacat gacgaccttc
     4021 cgcaccctcc tcgggaaccg ggagcttcgg ttcccctcgc ctgccggggt catggcgatc
     4081 ctgaacgtca cccccgactc cttctcggac ggcggcctcc acgcctcgac ggacgccgcc
     4141 gtcgccgccg cgctccgctt cggggcggcc ggcgccgccc tcctcgacgt cggcggcgag
     4201 tcgacccggc cgcgcgggac cgcctacggc ggcggcgcgg ccgaggtctc cgtcgaggag
     4261 gagctcgccc gcgtcctccc ggtcgtcgcc gggatccggg ccggcgaccc ggcggtcccg
     4321 atctccatcg acacgcggcg caccgaggtg gcgcggaaag ccctcgacgc cggcgccgac
     4381 gtcgtcaacc tcgtggccgg cctcgatccg ccgacggacc tcctcgagct cgttgccgag
     4441 agggacgcgg ccctcgtcct cggccacatg aggggcaccc ccgcgacgac gttcgaggtc
     4501 tcgcgcttcg gccccgacgt cgtggccgag gtggcgcacg acctggcccg ggcgcggcga
     4561 cgctgcctcg tcgccggcat ccgccccgac cagatcctcc tcgatcccgg cctcggcttc
     4621 gggaagacgg ccgagcagaa cttcgagctc ctcgcgcggc tcgaggagat cggcccggac
     4681 ggtgctccga tcgtcctcgg ggcctcccgg aaggcgttcc tcggctccgt ctcgggacgt
     4741 cccgccgccg agaggctccc ggaatcgctc gccgcgtgcg ccgtggcgat cgagaggctt
     4801 cgcgaccgga atcccgtcct gctgcgcgtt cacgacgtcg acgagacgct ccgtttcgtc
     4861 cacgtcctcg gcgccgccac cgctgcgggc cgtcgctagc ccgctcccgc cgccggtccc
     4921 gtaaactccg cgggtcatgc gcgcgctctt cggcacggac gggatccgcg gaagggccgg
     4981 agagtatccg ctcgacccgg ccaccgtctc ccgggtcggg ggggccctcg cgtccgtcct
     5041 ccgcgaggcc ggccgtccct ctcccctgac cgtcgtcctc ggctgcgaca cccgcgtctc
     5101 ctcgccgggg atcgtcgcgg ccctatcggg agggctgatc gccgccggtg ccggcgtgag
     5161 gttcgcgggg gtcgtcccga ctcccgcggt cgcccacctc gtccgcgccc tcggcgccga
     5221 cgcggggatt tccatctccg cgtcccacaa cccctggccg gacaacggga tcaaggtctt
     5281 ctcctcggag gggaggaagc tccccgacgc gctcgaggcc gagatcgagc ggcgcatcgc
     5341 gacctccgcg ccggccgagc cgatcccgcc ggccgaggac tcctctctcc cggagctcta
     5401 cgagaaccac ctggtcgaga cgctcccgaa acggctcgac gggctcaagg tggtcgtcga
     5461 ctgcgcgaac ggggccgcgt tccaggtcgc cccggaggcg ttccggcgcg ccggggccga
     5521 ggtcgtcccg ctcgcggccg ccccggacgg ccggaacatc aacgaagggt gcggcgccct
     5581 ccatccggag gcgatggcga acgcggtcgt ccgctccggc gcgcacctcg gcctcgcgct
     5641 cgacggcgac gccgaccggg cgatcctcgc cgacggagag ggacgcctgc tcgacggcga
     5701 cgacgtcctc ctcctctggg cgctcgagct cgagcgggag gggaagaagc cggggtcggt
     5761 cgtcgggacc gtcatgtcga actggggcct cgaggaggcc ctcgccgcgc gcggtatccg
     5821 gctcgtccgg gccgccgtag gggaccgcta cgtcgtcgaa gagatggaac ggcactccgc
     5881 tctcctgggg ggagagcagt caggacacct gattcgggcc gacctgacga cgacgggaga
     5941 cgggacgctg accggcctcc acgtcgccgc ggtcgtcgcg gccagcggaa tccccctcgc
     6001 cgccggcgcc cgcttcacgc gcgcccccca ggtcctccgc aacgtgaagg tccgggagaa
     6061 ggtcccgttc gagcgggtcc cgggtttccc gcacctcgtc gccggggccg agagccggat
     6121 cgccggatcg gggcggatcc tcgtccgcta ctccggaacg gagctcctcg cccgggtcat
     6181 ggtggagggg acggagcgcg ggacggtcga gtcgatcgcc gacgagctcg cgggcgcgct
     6241 ccagggtgct ctcggagcct cggactgacc gccggggaga gagagacgtg atccggcacg
     6301 agcagcgtcc ccggcgaaag cggagccccg aagcgatcga ggagcctgcc ccgacgctcc
     6361 tcgaaccgcc ggacgagccc gcgcccgccg agcccctggc cgagccgccc ggggccgtcg
     6421 aggagcgcgg cgaggtccga tcgcccgtgc ctctcctcaa ccgggagctc tcctggctcg
     6481 acttcaacca gagggtcctc gaggaggcgc gcgacgagcg gtggccgctg ctggagcggc
     6541 tgaagttcct ctgcatctcc gagagcaacc tcgacgagtt cttcatgatc cgggtctcgg
     6601 gcctccggga ccagctctcc gccgatctcg ccgaacgttc cgacgacggc ctgacggcgc
     6661 gggagcagct cgcgctcgtc cggaagggcg tcctcgcgat ggaggccgag cagacggcct
     6721 gcctccacga ggacctcttg ccccgcctcg cggcggccgg gatcacgctg ctctcgtggg
     6781 gagacctcga ggaggagcgg cgggaggcgg cgcggggtta cttccgcagg aacgtcctgc
     6841 cggtcctgac gcccctcgcc gtcgacccgg ggcatccgtt cccgttcctc tcgaacctct
     6901 cggtgaacct cgccatcgag accaggaacc ccgagaacgg cgaggtgaag ttcgcacgcg
     6961 tcaagatgcc tccggcgatc cctcgcctcc ttccgctccg gacggtcgtc gaggggaaga
     7021 agaaggtcaa ggccgcgcgc gcggagttcc tcctcctcga ggacctcgtc caggcgaacc
     7081 tcgacgagct cttcccgggc ctcgagatcc tcgcctcgta cctcttccgg atcacgcgcg
     7141 acgccgacat cgagatccag gaggacgagg cctccgacct cctcgccacg atcgagcagg
     7201 aggtccggcg gcggcggttc ggggccgtcg tccgcgtcga ggtgacgccg cagacgccga
     7261 agaagatgcg caagctcctg atgcggcagc tcgaggtgag cgagagcgac atctacgagg
     7321 tctccggcat cctcggcgcc gcggacctga tggccctcgt gaggctcgac cggcgcgacc
     7381 tgaaggacgc cccgttcacc ccggctctcc ccgccgcgct cgcgtcgacg gagggctcca
     7441 tcttccaggc gatccgccag ggggacctcc tcctccacca cccctacgac tcgttcgggc
     7501 cggtggtgga gctgatcgag gaggccgccg acgacccgaa gacgctcgcc atcaagatga
     7561 cgctctaccg gaccagctcc gactcccccg tcatccccgc cctcgtccgg gcggcggagg
     7621 cgggcaagca ggtcgccgtc ctggtcgagc tgaaggcgcg cttcgacgag gagaacaaca
     7681 tcgtctgggc caaggccctc gagcgcgcgg ggatccacgt cgtctacggc gtcgtcggcc
     7741 tgaagaccca cgcgaagatc gccctcgtcg tccggcgcga gaaggacgag atccgccgct
     7801 acgtccacct cgggaccggc aactacaacc cggcgacggc gaaggtctac accgacctcg
     7861 gcctcctcac ctgccgcccg gagttcggcg aggacgccac gcgcctcttc aacaccctca
     7921 ccggcctcgc gacgcggacc tcctacaaca ggatcgtcac cgccccgcgc gacctccacc
     7981 gggccgtcct ggagtggatc tcgcgcgaga cggagcacgc gcgcgcgggg cggcccgcgc
     8041 ggatccgggc gaagctgaac gcgctcgtcg acaccgcggt catcgaggcg ctctacgagg
     8101 cctcgcgggc cggagtcccg gtcgacctga tcgtacgcgg cacctgctgc ctcgtcccgg
     8161 gccttccggg cgtctcggag acggtccgtg tccggtcgat cgtcgggagg ttcctcgagc
     8221 acagccgctt cttcctcttc gagaacggcg gcgaccccga ggtctgggcc tcgtcggccg
     8281 actggatgcc ccggaacttc ttccggcgcg tcgagaccgc gttcccgatc gtcgaccccg
     8341 agctcggccg gcagctctcc gacgctttcg acgcgatgat ggccgacaac gcgagggcac
     8401 gcgcgcttcg ttccgacggg acctacgagc ggctccgccc cggccgcgga gagccggcca
     8461 tcgactcgca ggccttcttc ctcgaccagg cgcggcgccg cgtcctgaaa gccgcagagc
     8521 acttcgtcaa gggcgccgcc gccgacggct tcgaccgcgt ccccgacccg cgcgagcgcg
     8581 gccccgaaga cgaggaccgc tgacccgagc ggggacgggt cccccgctta ccgttcgagc
     8641 tcgaagcccg ccggcggcgt gagcaccggc tcggcgttca ggagcgccgt ttcggtgatc
     8701 gggaggagca tcgcgtgcgt gttcgacccg aactgcacgt ggacctcgta gtagccgttc
     8761 gccgaaacgg cgatgagcgt cccgggctgc ttcttcagct ccgcgctggg gcaggtgatg
     8821 tagaccttcg ccgggatgtc cattcggctc ctcctcgctc gtcgggctcg agaagattct
     8881 aagcggtggg cccgtcgccg tcgtcgtcgg cctcgtcctc gccctcgtcg ccccccgtct
     8941 cgccgcggta ctccttcagc ttccgctgga gcgtccggag gccgatctcg agccgttcgg
     9001 ccgccttgcg ccggttcccg ccggaagcct ggagagcggt gaggatcgcc tccttctcga
     9061 tctcggccat cgtcttgccg acgaggatcg ccgagtgcgc gatgggaacg gtcccctcgg
     9121 ggtggggccc ctctccctcc gagtctccct tcacgtcggg cgggaggtcg ccgacgtcga
     9181 tccgcgggcc cgcagcgctg aagacggccc attcgatggc gctcttcatc tcgcggacgt
     9241 tgccgggcca gtcgtacgac atgagcgccg agagcgccgc cggcgtgaat cccgtcacgg
     9301 ccctctcgtg ctccctggcg aacacctcgc ggaaccgctc ggccaggagc ggaacgtcct
     9361 cgcgcctctc gcggagggac ggcaggtcga cgacgacccc cttcaggcgg taatagaggt
     9421 cctgtcggaa gcgccccgcc tcgatctcct tcttcaggtc gcggttcgtc gccgagacga
     9481 ggcggaagtc gacctctttc gagtcgttcc cgccgacggg cgtgacgcgc cgctcctcca
     9541 ggacgcgcag gaacttcacc tgcaggtcgg gaggcatctc cgagacctcg tcgaggaaga
     9601 gcgtcccgcc gtgcgcctgc tcgatccgcc ccatccgctt cacgagcgcg ccggtgaaag
     9661 cccccttctc gtgcccgaag agctccgact cgatgatctc cttcgggatc gcgccgcaat
     9721 tgagcgccac gaacgcccgc ggccggcgcg gcgagttgtg gtggagcgcg ttcgcgacga
     9781 gctccttgcc cgtccccgac tctcccgtga tcaggacgtt catccgcgtc ggggcgacct
     9841 tccggaggac gtcgaagacc cgctccatcg ccgcggagac gccgatgatc gcctcgaagc
     9901 cgtagcgctt gtcgagacgc tcgcggagct gcttgatctc gcccgcctgg cgcgcgcgcc
     9961 cgagagcggc cgaggcgcgg aggcgcaggt cgtcgcgcgc gaccggcttg gccacgtagt
    10021 cgaccgtgcc gaggtccttg gccttcagga gcgtctcgat gtcgccgaag gcggtgacga
    10081 gcaggacgga gacgtcgggc cgcgccgccg cgagctccgt cgcgagctcg atgcccgtct
    10141 ttcccggcat cttcaggtcg gtcacgacga ggccgaccga cggatcgtcc atcgcgcgcg
    10201 agagggccga gtcggcgtcg gggaactcct cgaccctccg gccgtccgtc gtcagcgtca
    10261 gggccatcgc cttccggctc ccggcgtcgt cgtcgacgac gaggacgagc ggctcgcgag
    10321 gcggcgccgc gctcaccgcc cgcccccgat cgcccggtcc aggtcgtcct ccgaccagac
    10381 cggcacgccg agcgcgcggg ccttctcgag cttcgacccg gcttcgtctc cggccacgac
    10441 cgcggccgtc ttcctcgaaa cgcttccgct cactttgcct ccggctctct cgatcgccgc
    10501 cgtcgcctcc tcccgcgtcc gccgcgggag cgtccccgtc aggacgaagg tcatgccgga
    10561 caacggccct ccgggggcag gcgccccctc cggctcgttc atacggacgc cggcctctgc
    10621 gagtttagag aggaggcgga cgttcgccgg gagggtgaac cagtcccgaa cggagcgcgc
    10681 cgtctccggt ccgatctccg ggacggccgt cagctcctcc tcggtcgccg ccgagagcgc
    10741 ctccattgtt ccgaaccgcc gcgccaggac cttgccggtc ttctccccga cgtggcggat
    10801 cccgaggccg aagaggagcc ggccgagccc cgcctccttc gaccgctcga tctccccgag
    10861 gacgttcgcg gccgacttcc caccccagcg ctcgagcgcc gccaggtcct ccgcgcggag
    10921 cgcgtagagc cccgcgacgt cggcgatgag accggccgcg agaagctggt cgacccgttc
    10981 gtcgccgagc ccttcgacgt ccatcgcgcg gcgagcgacg tagtggcgga tcgcctcctt
    11041 gacctgggcg gggcacgagc cgctgacgca ccgaacggcg acctcgccct cctcgcggac
    11101 gaccgcctcg ccgcactccg gacaggccgc gggcatctcg aacgacacgg cgccttcggg
    11161 gcgcttctcg aggagaacgc gcgtcacttt cgggatgacg tccccggctt tctcgacggc
    11221 gaccgtgtcg ccgacgcgga cgtccttccg ggagaggtcc tcgtagttgt ggagcgtcgc
    11281 gcggcgaacg gtcgtcccgg cgaggaggac cggctcgaac tcggcgacgg gggtcaggac
    11341 gccggtccgg ccgacctgga cgacgattcg cgtcacgcgc gtcgacgcct cctcggccgg
    11401 gaacttgtag gcgagcgccc agcgcggcgc cttcgtcgtc gcgccgagac gctgctggtc
    11461 ggagacgggg ctcaccttca ggaccgcccc gtccgtctcg aggtcgaggg cgtgccgctt
    11521 ctcgcgccac tcggcgagaa aggccttcac ctccccggcg ctccgcgcga cggcgcggtg
    11581 ggggttgacg gggaggccga ggcgggcgag cgcgtcgagc gcctcgacct gcgtcgccgg
    11641 ccccccgtct ccctcccacc gcgcgacgga gtagaggtag accgagaggc ggcggcgcgc
    11701 cgtctcgcgc gagtcgagga gccggagcga gccggccgcg gcgttccgcg ggttcgcgaa
    11761 gaggggctcg cccgcctcct cgcgcgcggc gttcagcgcc cgaaaggccg ccttgccgag
    11821 gtagacctcg ccccgcacct cgaggaggcg gggggccccc tcggggagcc gaagcggcag
    11881 gacgcggatc gtcctcacgt tcggcgtcac gtcctcgccg gtcgtcccgt cgccacgcgt
    11941 ggcgccccga acgaggaccc cgtcctcgta gacgagcgag atcgagaggc cgtcgatctt
    12001 cagctcgcag acgacctccg ggaggcgccc gtcgagccgg tcggtcacgc gcgtcagcca
    12061 ggcgtcgagc tcagcctcgg agtaggcgtt ctcgagggag aggagcggca cctcgtggcg
    12121 gacgttcgga agatcgtcga cgggcgtccc gccgacgcgt cgtgttggcg agtcgggggt
    12181 gacgagatcg ggccaacgtt cctcgatcgc gaggagctct cgcgcgaggg cgtcgtactc
    12241 ggcgtcgctc acctcggggc gggcgagggc gtaatagagg tgctcgtgcc ggcggagctc
    12301 ggcgcgcagg aacgccgcgc gctccgtcgc cgccgcaacg gtctcgggga gcttcagcac
    12361 gccccggatt gtaggggccg cctcggcggg gtgacgggcg atactcccgc gtccgccggg
    12421 cgggccggcg gcgacggacg gatgaagcgg aagctcctcc tcgcaggcgg tatggtcgcg
    12481 ctcggtctcg ggctcgcggg cctcctgctc cccgtcctcc cgaccacccc gttcctgctc
    12541 ctggcggccg cgctcttcgc ccgcagctcg cccgagcggt acgagcgcct cctcgggcac
    12601 gagcggctcg ggccccacgt gcgcgactgg gtcgagcggc gcggcctgcg ccggagcgtg
    12661 aaggtccggg cgatcctcct cctctgggcg gccttgctgc cgagctccct ctggctgggg
    12721 caccgtcacc tttccggctt cctcctcctc gccgtcggcg ccgccgtgtc cgcctatctc
    12781 ctccgcctcc cgacgctcga ggagccgccc gcggacgcgg ctcttcccaa gagcggcgac
    12841 gccacctagc atcgcggccg tgtcctcgcc caaagcgttc ctggggctcg tcctcctcgt
    12901 tgccggcctg cccgcccgcg ccggggagcg cgtcccgccc gtcgcctcgt atcgcatcga
    12961 ggcgagctgg gacgaggagc ggaaggtgct cgccgggcgc gagacgatca cgttcgtcaa
    13021 ccggaccacg cgccccttcg acgacgtggt ccttcacctc tacctgaatg gcttccgcaa
    13081 tacccgttcg acgctctacc gggccctgcc ccggccgacg ggctccgacg ggttcggcta
    13141 ctgcgagctg aagcggatcg ccccctccgg cggcgaggat ctgacgcgcc gggtcacgtt
    13201 cgcgtcgggc ggtgccgaca acccggacga ccggacgctc gtccgtgtcc cgctgacgcg
    13261 ccccgtggcg cccggagaga cgctcgtcct cgaggtcgac ttcgagtccc ggctcccgcg
    13321 gggcctcctg cggacggggt ggaaggacga cttcgtcttc gccggacagt ggttcccgaa
    13381 gctcgcgaag gcgacggagg cgggctggcg cgggaggccc ttccacccct tcacggagtt
    13441 cttcgccgac ttcggcgact atgacgtgac gctcctcctg ccggccgagg tgaaggggcg
    13501 cgtcgccgcc accggccgag tcgtcgaaga gtccgacgcg gacgcggggc gcgtccgcgt
    13561 gagggcccgc gcggaagacg tccacgactt cgccttcgcg ttctcgcccc gattcgaggt
    13621 gaagacggag aaagttgcgc cgaaggggct ccccgcggtg gaggtccggc tctacctcca
    13681 gcccgaccac cgccgctcgc gcgaacgcta cctgcgcgcc gcccgggagg gcctcgcgca
    13741 ctacggcagc tggctcgggc cgtatccgta tcccgtactg acgatcgtcg accccccgac
    13801 cggctcggga accggggcga tggagtaccc gacgctcctc accggcggaa cgacatggct
    13861 ctccccggcc gccggcggag agccgggcgc cgtcgtcctc cacgagctcg cgcaccagtg
    13921 gttccagggc gtcgtcgcga ccgacgaggt gagcgaggcg cacctcgacg aggggctcgc
    13981 gacctgggcc gccgcccgcg ccgtcacgcg gctgcaggga ccgccgagcc cggtcgtcga
    14041 ggcgttcggg atcccgatcg ccctccgctc cctctcgcga acgctccccg agggggaggc
    14101 gcaggacgct ctcgaccagg cggccttctc gcgctccgac ccgacgctcc gcgagtcgtg
    14161 gcgcgccctc gacgacgcgg cggtccggac gaacgcctac tcgcggacgg cgctcctcct
    14221 cgagtccgtc gcccgcacga tcggcgatca gccgctccgg gccgccgtcg gcgagtacgt
    14281 ccgccggttc gcgttccggc atccgacgac gacggacttc ctcgacgtcg tcggggaggt
    14341 ggcgggagcg gaggcgcgcg cgctcctcga gcgcggatgg gcttcggccg gtcccgccga
    14401 ctacgccgtc acgaaggccg agaccgcccg gatgcgccct ccggccggct tcctcggcga
    14461 ggggccgagg agggcgtggg cggcgccgac ccagggcagg ggcgagtggg cgtcgacggt
    14521 cgtcgtccag cgtctcggcg aggtggcctg gccggtcgtg gtggagctcc ggttcgaggg
    14581 gggccacgtc gtgaagcgga cgtgggacgg gagaggcgag tggatccgct atcgcgccac
    14641 cggaccgagg ctcctgtcgg ccgtcgtcga cccgggccgc gcctgcctcc ttgacgtcaa
    14701 ccggctgaac gacggcctcg cgacggagcg cgatccgagg gcggcgcggg cctggggaca
    14761 ccgactccgc ttcctcgctc agaacgtcct cgagctcttc gcgctcgtcg cagccctccc
    14821 gggggccgca cggtgaaggg cgatgcgctc cccgtcctct gggcgagggg actcctctcg
    14881 gctgcacgga cctggaaggc gctcctcctg gccctcgcgc tgaacgccgc gctcgcgctc
    14941 gtcgtgaccg cgccgctcgc ctcgcgcctc cacgacctgc tcgacgagcg gccgacggcc
    15001 gcgccgctcg cgaacggccg ggacctcgtc ctcgaagcgc acctcctccg tgccgagtcc
    15061 gggctcctcg gcgggagggc gcgcctcgag gcgctcgggc ggggggagct ccttctcgag
    15121 gagctgccgc tcctctccgg acccctcgcg ggccttctca ccgcgggcct gctgcacgcc
    15181 gcgctctcgg gcctcctcgc gggcggcttc gccggccgct tcgcttcgga gagggaccgc
    15241 ggcagcctcg ccgcgttcct ctcggacgtg gtgaggctcg gccttccgtc gctcgtcctc
    15301 ggcctcctcg gcctcgccgg gatcctcggc gcgttctggg tcttcgtgga gctcccgcgc
    15361 gccgcgacgc tctcgcccga cctccgctgg gaccaggaga ggaccggcct ctcgctcctc
    15421 cggctcggcg ccttcctcct cgcggccggc gcgatccggg ccgtcgtcgc gcagtcgcgc
    15481 gccgcgctcg gcctctcgcg ctccgtgaac cctctcgccg ccctgggcgc ggggctcggc
    15541 ttcgtcctcg gccgccccgt gaaggcgctc ctcctcgagg tcgccttcgg cgcgacggcg
    15601 ctcctcccgc tcgccctctg gctcctcttc gcgccgtccc tgggcggcgg cgacctccgc
    15661 ctcctcgcga ccctcgtcgc cgggcagcag gtcgtcgtcc tcctccggat cgcatcgcga
    15721 acggcccacc tcggagccgc ctccgcctgg atgcgcgggg cgcgcgaggc ctctcgcccg
    15781 gcgcctgcga agatcgagtc gtgatcccgc tccgcacgaa gactcccggg tcctgggcgg
    15841 agagggccgc ggcccgcctt cccgagctcg tcgccgacca cgcggcgtgc gagcttcagg
    15901 ccgccgtctt cgccctcgcc ctcgtcgggc gctaccccgg cgacccgctg ctcgtcgacc
    15961 gcctctcggc cctcgccgcg gaggagctgc gccacttccg gaaggcgatg cgggaggcgc
    16021 ggcgactcgg cgcgagagtc ggcacgcgcc gcgcgaacgc ctacgtctcg gtgctgcggg
    16081 ccgccggacg cgccgggcgc gagccggagc aggggctcga cctcctcctc tgcggcgccc
    16141 tcgtcgaggc ccggagccac gagaggttcc tcgcgctcgt gccgcggctc gccgcggagc
    16201 cgcgcctcgc gaagctctac gtcgagctcg cggccgccga ggagcggcac gggccggcgt
    16261 acctcgagct cgccgagcga cacgccggaa gggaggcgac ggcggcccgg ctcgaggagc
    16321 tcctcgacgt cgaggcgcgc gcgatcgcgg gcgcgccgac ctcgccggtc gccgtccact
    16381 ccggggcgtg atcgccttcg tccgtcagtc tccgtcgccg cccggcagag agtgtcgggc
    16441 gatgacgaac tcctccacgg gtctcccgcc aacgagatgc tcgtccatga tcctgtcgag
    16501 gaccggcgga tcgcaggccc ggtaccagat cccctcgggg tagatgacgg cgatcggccc
    16561 cctctcgcag acccgaaggc agccgacccg ggtccgtagg acgcctcccc tctccgagag
    16621 gccccgtcgc ttgaggccgt ccttcaggtg cgtccacgcg gccatcgcgc gttccgcgtc
    16681 ggcgcacttc gggttctcgc gatccgcgca gaggaagacg tgccggcggg cgacgtcgac
    16741 gccggccctc tcgagcttgc gggcgatgat ctcggcctct ccgagcggct tgccgtccat
    16801 ggcgtcctcc gtcgggcggg aagtgtaggc gagcgcccga agggcgcgtc ccggcggctt
    16861 cccggtcgag tcccgcccgc gccccggtat agacttcccg gactccccgg aacgcccgac
    16921 ccaaggagat ggcgatgggc ctcctgaagg actttctccg gatcgacgcc gaccggcgct
    16981 ccgccctcgc gacggttcgc caggaagcgc agtctcaccg gctccgcatc gaggagctcg
    17041 gcgcggaggc gcggcgcaaa cgggacgaga tcggaatgct ggaggaggag ctccgtcggc
    17101 tcgccgagga cgtggcccgg acggaggagc tcctcctcga cctggaggcc cgccgcgagg
    17161 cgcacgaccg ggacacacac gagcgcaagg tcgacgcggt ccggtcgggg ctcgcgcacg
    17221 accgtgccga cggtttccgg atcgcggagg agttccgtca gctccgcggc acgttcgaag
    17281 gggagcgcac gcgcctcctc gccgaggccg acaccggccg gatgatggac aacttcttcc
    17341 agatcgaggc gttcctgaaa gacaccggga cgccgatccc cgacgcggcc cgcaaggcgc
    17401 tgatgaagga gcggcaggac ctgatggcgc ggatcggccc gctcgtcgcg ccgcctcccg
    17461 tgcccgatgg ggtcttcaag gcgaccgtcg tctactcgac tctcgaggag ggcgagccga
    17521 ccgccgtcgt cgcggtcggc ctccccgacg aagccgacgt ctccgggccg cacgacctct
    17581 cggcccttct cctgtacgcg tcgtacgcgt cggtcgtcga gaagatcggc cccggagtgc
    17641 cgcggccgcg ccggtcggac ggcgtcgtcg tctacgagca gccggccggc tcccgccccg
    17701 ccgacgaggt cgcgctcgac ctcttcctcg ccgtcgagga cgggctgcgg aagggcgccg
    17761 cagcggccgg cgtcccgtgc gagctgaccg gagtcttcct cgagcccgag atcgcttcgg
    17821 cggtctttcc ccgcggcgac gcccgcggca tctgaccgga ggctgccatg accgtgaagt
    17881 ggatcggcga cgccgcccag gagctcggcc tctcccctct cgacgccgtc gagatcctcg
    17941 cgtcgcgcca ggagtacccg atgaacgggc tcctggacga ggaccggttc aagaccctcc
    18001 ggctgatgcg cgggagcaag cggctcgaga cgtccggctc ccagccgatg ccggtccccg
    18061 accggctcgc ctccccctcg acggtcccga tcccgacgcc tccgccgcag gcgcacgccg
    18121 ctcctccccc ggcgcccgtc gtcacggctc cgccgccggt ccccgagccg ttcgctccgg
    18181 cgccgccgcc cgttcggccg ccggtcggcg agcggacctc cgtgacggcg ccgctccaga
    18241 ccatcccggg acgcgagggg gagaagacgg tcatcctctc gccgatcgag ccgaaggagt
    18301 gagcccggcg ccgcccggcg ggcggcgcct cgccgccttc acgaccggga ggccgccgac
    18361 ctggccgcga ccgcgcggcc tcccgaagcc gggaccagga gctcccgcac ctggcgtgcg
    18421 agctcgagac agcaggtgag cccgggcgac tcgatgccga cgaggttcac ccatcccggc
    18481 agcccccgct ccgactcctc ggcgacgacg aagtcgcgga acggcgcgcc gatcacctcg
    18541 aggcgcgcgc ggatgccgga ctggtcgggt gagaggtcgt ccgcgacgag cgcggggagg
    18601 tagcggcgcg ccgcggcgag gaaggccccg cgccgcgatt cgtcgaccgc atagtccgcg
    18661 cggcgctcct cgagcggctc gacgtcgggt ccgagcttca gctccccgtc gaggtcgacg
    18721 gtcaggtgga tgccgaggac gcttccgtag ccgtgcggca tcaccgggta gaggaggcgg
    18781 cgcgcgagat ggcggcggga ggatcggacg cggaagtagc tccccttcgt ccaggtctgg
    18841 cggtagcccg cggcatcgac gtcgagcccg ggcatcgcgg cgacgacgtc ggcgtcgagt
    18901 ccggcggcgt tgacgacgcg cggcgtcgag aggcggacct cctcgcccga cgggtcgacg
    18961 aagacgagct cgtacccgcc gcgcgccggc gcggccgcct tcagcacgtg gcggaacgcg
    19021 aagagcgcgc cgttctcctc cgcgaccgcc cggaacacgc ggaacaggcc gtggacgtcg
    19081 aggatcccgg tcgagggcga gaggacggcc gccgtcgcgc ggacgtgagg ctcctcctcg
    19141 cggaagcgag agagagaaac ctcctcgagg agagcgccgt tctccctccc cgcggtgacg
    19201 atctgggcga ggcgctcctc ctccccgtcc tcgagcgcga ggagccactt ccccgtccgc
    19261 cggaacggga cgccgtgcgc ctcgcagaag gccgcgaggt tccggttccc ggcgacgcag
    19321 agccgcgcct tccgcgaccc gggcgggtag tagagccccg cgtggaggac ctggctgttg
    19381 cgggagctcg tctcgcgccc cggtcccggg tggcgctcgg ccaccacgac cgagagcccc
    19441 gcgcgagaga gctccgccgc agtggcgaga ccgaccaccc cggcgccgac gacgacgacg
    19501 tccgcgtcca ccgcgccgat cgtctcaccc cttccgcgcc ccgtcccgcg cggaagagca
    19561 tcgcgcgccg ggatcgtgta aaacgtcgcc gccccgcatg agcccgaccc ctccggacgc
    19621 ccccggcgcc gtgaccgccc gcctcgcggc gcggggcctc gccatcctca cgctcgtcaa
    19681 cctcttcaac tacctcgacc ggttcgtcgt cgcctcgctc gtcgagagcc tgcggaattc
    19741 cgagctgcgg ctgaccgaca cgcaggccgg cgcgctgatg accggcttca tcgtcgtcta
    19801 catggtcgcc tcgccgttct tcggcgtcct cggcgaccgc ggctcccgcc cgcgcctcct
    19861 cgcgctcggc gtcttcgtct ggagcctcgc cacggcggcc gcgggtctcg ctcggagctt
    19921 cgcgtcgctc ttcgcggcgc gggcggccgt cggcatcggc gaggcggcct acggcacgat
    19981 cgccccgagc ctcctcgccg actatttccc gagggagaag cgggggcgcg tctacgcgat
    20041 cttcttctgc gcgatcccga tcggctccgc gctcggctac gtcgtcggcg gcctcgccga
    20101 cacgcacctc ggctggcgcg ccgcgttcta cgtcgccggc ctccccgggc tcctcctcgc
    20161 cctcctcgcg ctgacgctcc ccgacccgcc acgtggcgtg acggacgccg ccgaggcgcc
    20221 gcccccgggg acgccgaagg actccggctg gtcctcctac ctcgccctcg cgaagaaccg
    20281 tcccttccgg ctcgccgtcc tcggctacgc cgcctacacg ttcggcctcg gcgcgatggc
    20341 gttctggatg cccgcgttcc tcgagcgggt gcggggcgtg ccgaaggaga cggccaccat
    20401 ccggttcggc ctcgtcgtcg tcgtcaccgg cttcgtcggc acgttcgccg gaggcttcct
    20461 cggcgacgcc ctcctgaaga ggacgaagga ggcctacctc tgggtctccg gcgtcgccac
    20521 gctcctcgcc gtcccgttcg ccgtcgtcgc gctcacggcc gcggcgccgg gcgtctactt
    20581 cccggccctc gtcgtcgcgc agctcctcgt cttcgcctcc acggggccga tcaactccgc
    20641 gatcgtcaac gacgtcctcc cgtggcagcg ggcggcggcg gtcgcggcgt gcaacttcac
    20701 gatccacgtc ctcggcgacg tcccgtcccc tccgctcctc ggcgccgtct ccgacgcgtc
    20761 cacgctcgcc aacgccgtcc tcctcgtccc ggccgcgttc ctcgtggggg gcgcgatctg
    20821 gacctggggc gcgctcacgg ggcggcgcgc gtgatccgca ccctcgcgac cgccgtctcg
    20881 cgcctcgtcc tgcgcgtctt ctaccggaac gtcgaggcgg ccggcctcga ccgggtcccg
    20941 gccgcggggc ccacgatcct cgtcctgaac cacccgaacg gcctcatgga cccgctcctc
    21001 ctcctctgcc tctcgccgcg ccccgtctcg ttcctcgcga agtcgccgct cttcacgatg
    21061 ccgttcgtct ccctcttcgt gaaggcgttc gactcgatcc cggtctacag gccgcaggac
    21121 gccgacgccg acgtgcggcg caaccgcgag accttcgcga aggcgcgcga gatgctgaag
    21181 cggggcgggg tcctcgccct cttccccgag ggggtctcgc acgacgagcc gcgcctgatg
    21241 ccgctcaagt cgggcgcggc acggatcgcc ctcggcgccg cggccgaggg cgccgtcacc
    21301 gtcgtccccg ccggcctcac ctacgaggcg cgcgacctct tccggagctc ggccctcctc
    21361 cacgtcgccg agccctttac cgtcgagccg atcccgctcg acgagaaggg ggagccgccg
    21421 cgcacggccg tgaaggcgct gaccgagcgg ctccgcgaag cgctggaggg ggcgacgctc
    21481 caggccgacg acgcggcgac gctcgagctc gccgcgttcg ccgagtcgct cctgcccgcc
    21541 ggggacggca gcctcgaggc gcgcgtcgcc gtccgccggc tcctcctcga gcgggccgcg
    21601 ctccttcgcg accgcgcgcc ggaacggctc gcggccctcg tcgcccgcct cgagaggttc
    21661 cgcgccatcc tcacggcggc ccgcctcgcg ccaggtgcgc tcgattccgg cgcctcgcgc
    21721 gccgagctcg ccgcctccac gctcgccgcg ctcctcgtcg ccctcttcct cgccccgccg
    21781 gcccttctcg ggctcctcgc ccacgccccg gcctacaacc tcgtcggtcc gctcgcgaag
    21841 cgcctctccc gcgggaacac cgacatcgtc gcgacgatca aggtcctcgg cgcgttcctc
    21901 ttcttccccc tcacgtggat cgcgctcggg acggcggccg ggctcgcctg gggcctcccg
    21961 ctcggcctcc tcaccggcct cgcgctcccc gtcctcggct acctcgcgct cctcctcctc
    22021 gagcgctggg aggcgacggt cgccgccctg cgcgccctcg gcctcgccct cgtccgccgc
    22081 ccggccctcg cccgcctccg cgccgagcgc gagtccctcg tcgccgagat ggccgccctc
    22141 gacgcagagt gtagagtgta gaccagaccc catcccaaga aggggtcagg tctaccatct
    22201 acagtctacg gggatcaggc gagggcgagg agggcctcct ccacggcgcc cttcacgagc
    22261 gggaccttgt agtcgttgcc cgacatcggt tcggccccct tcacggcgag ctcggccgcc
    22321 tcggcggcga gcttcgcgtc gagggccttg ccgacgagga gcttctcgac ctccgggacg
    22381 cgccacggcg tcggggcgac gcccgagagg acgacgccgg ccgaggcgac cttcccgtcc
    22441 ttcaccgaga gggcgagggc gacgccgccg agggcgaagt cccacgcgcc gcgcgagcgg
    22501 actttccggt agtggccttt ccccccttcg agcggcggcg ggagaaggac ctcggtgagg
    22561 acctcgcccg gcgcgaggac cgtctcgcgg gtgtggtcga cctcgggcag gacgaagagc
    22621 ttctcgacgg gaagggtccg gacccccttc ggcccgacga ccttcaccgt ggcgcgaagc
    22681 gcgacgagcg gggaagcggt gtcggagggg tggacgatgt agcagccgga gccgccgaag
    22741 acggcgtggt aggtgttctc cccctccacc gcgtagcaga tctcgccccc cttgcgggcg
    22801 cagtcgaagt cgccgcggaa gtaccagcag cgcgggcgct ggcagaggtt cccgccgagc
    22861 gtcccctggt tcctcagctg ggggctcgcg gcggcggcgg ccccttgcgc gagggcgggc
    22921 cagcccttcg cgatcccggc atgccgggcg acctcggcga gcgtcgtcag cgcgccgatc
    22981 cggaggcccc cgtccttcgt cgccgagatc cctttcaggt cggcgaggcc cgagaggctg
    23041 acgacggact tcgcgtcgaa gacgccgtcc cgaaggcagc cgaggagatc cgtgccgccg
    23101 gcgtgggcgc gggccccctc ggccgcgagc gccttcacgg cctcggcgac ggtgcgggga
    23161 cggacgtatc gaaagctcgg aagcatcgcg tcaccccttc ttcccggccg cggcgaggag
    23221 cgcggcgagc ttcggcggcg tgatcggaga gtcgtggacc cggaggccga cggcgtcgcg
    23281 gaccgcgttc gcgatcgccg cggcggtcgg gatcgtcgcc ggctcgccga cccccttcgc
    23341 ccccgcggtg ttgcactcgg tgtcgacgag gtcggcggag aggacctcct gcgccgccgg
    23401 gacgtcgagc gccgtcggga tcttgtagtc gtgccagttc gcgttgacca tcttcccggt
    23461 ctgccggtcg aggacccgct cctcggtccg gccgaggccg aggcccatca cgaggccgcc
    23521 ctggacctgg cactcgaacg tcaggcggtt catgacgcgc gcgctgtcct gagcggcgac
    23581 gagcttcgtg acggccacct cgcccgtccg cacgttcacc tcgacctcgg cgaagtgcgc
    23641 cgcgaacatc gagatgacct tcccctcgac gttcggtccg cgcgtcccga cgccgacgag
    23701 gacctggttc cgctgcagcg ccggcaccgc ggtaacggcg agcttcttcg ccgggtcctt
    23761 cgtcgagacg acgtcgtttc cgtccagctt cagctccgcg acggggacct tcagctcctc
    23821 cgctgccccg gcgaggagct tcgtccgcac ctcgatcgcc gcggcgcgcg ccgccggcat
    23881 gtcggacggg acggtcttcg agccgccgct cggcccgctg tattgcgtcg tgccggtgtc
    23941 cgcgtgctcg atctggatct tctcgagcgg gacgcccagc tcctcggcga cggtctgggc
    24001 catcacggtc ttcgtcccgg tgccgatgtc ggcggccccg gagttcacgt tgacgctccc
    24061 gtccgcgtag agcttcacga cgaccgtcgc cggcgggccg cctccgtagc cccacatccc
    24121 ggccgcgacg ccgtacccct tccggatcgg cccgtcgtcc ttcctcttct cccgcgtcgt
    24181 ctcccacccg aacgccttcg cgccgcccga gaggcactcc gcgaggccgg tggaggtgta
    24241 cgggaggttg ccgcgccgct ggctcacctt cggcacgttc gcgagtcgga gcgcgaccgg
    24301 gtccataccc aacttcagcg ccagctcgtc catcgcctgc tcgagcgccc aggccccctg
    24361 cgggaagccg ggggcgcgca tcgcgcgcgc gcgtccggcg ttgatcgcga cgttctcgtc
    24421 gacggccgcg acgttcgggc aggtgtagag gtccgtcgcc aggaacgacg tgtcggctcc
    24481 gttcggatag gcgccgcccg agccgatcga ggagaactcg atcgccgtca gcgtgccgtc
    24541 cttcttcgcg ccgatcttca ccttcatcgt gttcgcgggg cggttccccg cgttcaggaa
    24601 gacctcctcg cgcgtcagga agagcttcac ggggcgggcg gtcctcttcg cgaggagcgc
    24661 ggcgatgacc gtgtacttcc ccgcctcgag cttcgacccg aagccgcccc ccatgtagtg
    24721 accgatcacg cggacgttcg cgagcggcag cttcagggcc gcggcgaccg tctgctggac
    24781 gacgaagacc ccctgggtcg agtcccagac cgtcaaccgg ttcccgtccc acttcgcgac
    24841 ggagccgtgg acctccatcg gcgcgtggat ctcgcacgcg gtcgagtacg tcccctcgac
    24901 gacgacgtcg gccgccgcga agcccgccgc gaggtcgccc cgctcgtacc gggccggctc
    24961 gccgtagcgg ttcccgccgt cgtggacgag cggggcgtcc ggcgcgagcg cctcgcgcgg
    25021 gtcgaggacg aacgcgaact cctcgtactc gacgcggatc gccttcagcg cgtcgaacgc
    25081 ctgggcgcgc gtctccgcgg cgaccgcggc gacctcgtcc ccctcgtggc gcacccgctc
    25141 gtcgaagagc ttcgagtaga accgcccccg gctccggtac cacggcacgt cggcgccggg
    25201 agagaacgcg gtgaggacgg cgcggacccc cggcatcttc tcggccgcgc tcgtgtcgat
    25261 cgccttcacc cgggcgtgcg cgtgagggga gcggaggatc gcggcgtgga gcatgtcggg
    25321 gagggcgaag tcgagcgggt agacggccga gccgctcacg cgctcgtagc cgtcgaccct
    25381 cgggacgggc ttccccacga ccttcgtctc gccccacgga ggcatctcgg ccttcggcgg
    25441 ctcggtctcg gggacggggc cctggagcca gtagaggagg tccgtcacgg cgtcacctct
    25501 ccttccggag ccgggcggcc ttgtcgaccg ccttgaagat gtgggcgtag gcgccgcacc
    25561 ggcagaggtt gccgctcatg ccgtggcgga tctcgtcgag cccgggctcg gggttctccc
    25621 ggagcagccc ctccgcggcc atcacctggc ccggcgtgca gtagccgcac tggtaggcgt
    25681 cctcctcgac gaaggccttc tggacgggcc cgagctcctc cccctccatc agcccctcga
    25741 ccgtcgtcac cttcttcccg tcggcctcga gcgcgagcgt gaggcaggag tagcgcgcca
    25801 cgccgtcgat caggaccgtg cacgcgccgc actcgccccg ctcgcagccc ggcttcgtcc
    25861 cggtgaggcc gagcttctcg cggaggacgg agaggagcgt gtcgcgcggc tcgacgagga
    25921 ccttgtgcgt cctcccgttc acgacgagcg tcagcttcgt cggctccgcc gcggggagct
    25981 ccgcgatcgc ggctggcgcc gcggcgggag cgccgagcgc ctccttcgcc accgcgacgc
    26041 cgacggccgc cgggaccacg gagccgagga atccccggcg ggaaacgccg ccgttcgtgt
    26101 cgaggtcgtt ccggtcgtcg cttctctcgg ccatgcgtcc tcccggggcc ggactcggcg
    26161 cgcggcggct ccggcccgat cgggcatgtt acgacgcgac ggagctccct tccgcgtcgc
    26221 ggaagatgcg acggcctctc gggactgcgc atccgctcgt ccgaccgcac gtgtgtcgac
    26281 gccggccccg gcccggccgg agacctcgtc gtcgagagga cctactcgcc gatccgggcg
    26341 cgatgctcga cggccccgga caactccgcg tcgcggccga gcgccaccgc gagctccgcc
    26401 gcctcgtccc ccgcgaggac gtggacgacg ccgccgggga ggcgcatgac cccgtccgcg
    26461 ccggcagccc ggactccggc ctcgtcgacg cgcgccgggt ccgcgaggac aacacgcacg
    26521 cgggtccgcg cgcacgcctc gacggtccgg acgtttccgg cgcctccgag ggcctcgacc
    26581 agccggcgcc cgcgcgcggc gacgtcgggc gagacggccc catgcgggcc cgacggctcg
    26641 cgcggtgccg ggccggcgcg tcccccgagc accccttcca tggccgcctt gaggttctcc
    26701 gagcgcgtcc cgtagaccgc ctgcaccgcg ttcccgagga cgacgactcc ggccgccccg
    26761 agagccccga gctccccttc gtcgacccgg tcgaggtcct gcaccgtgac gcggaggcgc
    26821 gtgatgcagg cgtcgaggct ctcgatgttg cgggctcccc cgaaggcggc gacgagcctc
    26881 tcggcgattc cctccgatcg gccgggctcc gcggccggtg tcgggctctc gggccgggcc
    26941 gcgggggtcg cgggctcctc ccgccccggc gtccgcaggt caaggcggac gatcgccgcc
    27001 cggaagagcg cgtagtagac gagcgcccag agcggcccga ggagcggcac gagccagggt
    27061 cgggtcccca tcggccagta gaggacgagg tcgaacaggc cgtgcgagaa cgtgaagccg
    27121 agcttcatcc ccgcgagctc cgcgacgagg taggagagcc ccgcgaggag ggcgtgcagg
    27181 gcgtagagga acggcgcgag gaagaggaac gcgaactcga tcggctcggt cacgccggtc
    27241 aggaacgagg tcgcggccgc ggagagcatc accgcgccca cgcgcgcgcg ccggtcctcc
    27301 cgggcggagc gccagatcgc gagcgccgcg gcgggaaggc cccacatctt gaagaggaac
    27361 ccgccgccga ggatccccgc ccgcgggtcc cccgcgaaga agcgcgggat ctcgccgtgg
    27421 atcgcggtcc cggcgcggtc gacgtacgtc ccgagctcga agaagaacgg gacgttccag
    27481 atgtggtgga ggccgaacgg caggaggagc cggtcgacga cgccgtagga aaagaccgcg
    27541 gccgcgggtc ccgcctcgag ggcgcggtcg gagaggaggc ggaccgcgtc gctcagcgga
    27601 ggccagacga cggagagcgc caggccgagg agcgtcgccg cgaggcccgt caggatcggg
    27661 acgagccgag gcccgccgaa gaacccgagc gccgccggaa ggtccgaccg cgagaaccgc
    27721 cggaagagcg cggcggcgac gaggccgacg aggatcccgc cgaagactcc cgtctccacc
    27781 gattcgaagc cgaggacccg caccgtcggc aggccgagcg cgttcgcgcc cgcgcccaga
    27841 gtggcgacga agacgacgta gccgacgacc gcggcgaccg ccgcggcgcc gtcgttcccg
    27901 gagaggccga gcgccaccgc gatcgcgaag acgagcggga gagccgcgaa gacggcgccc
    27961 ccggcctccg cgaggaggtg cgccgcgccg gccgggagga ggccgagccg ggcgttcccc
    28021 aggccgagca gcagcccggc gacggggagg accgagaccg ggagcatcag cgcccggccg
    28081 atcgtctgga gctgcgcgag agcgccgccc cccgtcacgc cgcgccccac gaggccgcga
    28141 ggagcgcgcg cacctcggcc gccgtccccg cctcgagcgc ccccacggcc agagaccggc
    28201 actcctcgag cgagagctcc cggacccgcg ccttcacgga cgggacctgg gccgccccga
    28261 cggagagctc tccgaccccg aggccgagga ggagcggcac cgccgcgagg tctcccgcca
    28321 gcgcgccgca gaccgccacc gggactcctc gcttccgtgc cgcggcggcg gtgcgcgcca
    28381 ccaggtggag gacggccggg tcgagcgcgt cggcccgcgg agagaggcgc ggatgcccgc
    28441 ggtccatcgc gagcgtgaac tgcgtcaggt cgttcgtccc gatcgtggcg aaccccgcct
    28501 cgcgcaggaa cgcgtccgcg aggatcgcgg ccgaggccac ctccacggtc atcccggcca
    28561 ccctcggcgg cgcccccagc gccgcgcgct cttcctcgac gtccgcgagc gccccgcgcc
    28621 actcgtcgag cgtcgcgacc atcgggaacg tcaccctcac gtcgtactcc ggaggcaccc
    28681 tgaggagcgc ccgcagctgg gcgcggaaga ggtcggggcg gtcgatgaag acgcgcaggc
    28741 cgcgctcccc gagagccggg ttctcctcct ccggcagcgg gagccagcgg agcggcttgt
    28801 ctcccccgac gtcaaacgtc cggacgacga gcggcctccc cggccccacc gcctccgcga
    28861 cggcccggta aaccgcctcc tgctcgtcct ccgtcgggac gttcgcccga tcgaggaaga
    28921 gcagctcgga tcggagcagc ccgattccct ccgcgcccgc cgcgatcgcg tcctccgcgt
    28981 cggccaggct ccccgcgttg ccgaggaccg tcacgcggac gccgtcccgc gtccgggcgg
    29041 gctcgagcgc ggccaggcgg tccgccgtcc gtcgcgccgc gcgccgcgcg cagcgctccc
    29101 gcacggcctc gcgctccgcc tcgtcgggag cgaggcgcag ctcgccggca tccccgtcga
    29161 ggacgcccgg cacgcccggc tccacctcga gggcgcgggc gtcgatcccg gcgaccgccg
    29221 ggatccccat cgaccgcgcg agcagcgcca cgtgcgacgt ggcccctccg gcgacggtgc
    29281 agaacccggc gacgccgccc cggccgagcg tggccgcctc agacggcgtc aggtcctcgc
    29341 tcacgacgat cgccccttcc ggcggggccg gggcggacga gcgcgacccc gcgaggatcc
    29401 ggaggacgcg gcctcccgcg tcgcgcaggt cggcggcccg cgccgccagc gccgggttcg
    29461 ggaggctcgc gatccggtcc gagtagcgcc gcaccgcctc ccggaacgcg aacgccgcgc
    29521 tcttgccgga ggcgatctcg tgctcggctt ccgagaggag gagggggtcc tccagcagcg
    29581 ccacgtgcgc ctcgaagatg tcggcgtgct cgctccccgc ctcggcgccg agccgcgcct
    29641 cgagggcgcg gagctggacc ttcgcctcct cgagcgcgaa gtcgaggtcg cggcgctcgg
    29701 tccgcccgtc gctcccctcc tcgacgacct ccggctcctc gcgcgcgagg cggacgaccg
    29761 gcccggccgc gcagccgggc gccgacggga tcccggggaa gacccgggcg cccgtccgga
    29821 ccgggcgcgg ggccgacggg agcggcgccg ccgcctcgtg cccctcctcc gcctcgagga
    29881 cccgggcgag ggccgagagc gccttgcccg cgtcgggacc cgtcgcgcgg agccgcacga
    29941 cctcgccgtg gacgacgccg agcgagagga ggccgacgat gctccgcgcg ctcgccatcg
    30001 acgtccccgt ctcgacgagc acctccgccc ggaagcggcg cgccgccgaa gcgagcgccg
    30061 ccgcgggccg cgcgtggagg ccgagacggc gcatcacccg tacgtcgcgc gtccgctcgc
    30121 cggccgccgg cgccacgagg gccgacgccg aagggacgag cgtgaggacc atgaggtcgt
    30181 ccacccccgc gacgaccggg cccccggcga cgcgcgccga cgtcacccgc tcgcggctcg
    30241 tcacgaccac aggcgtcacg aggctccggg ccgagcgggc gacgaggtcg gcgtcgaacg
    30301 ccaggagcgg ctcgccggcg acgacccgcc gcccctgcgc gacgcgcggc acgaacccgc
    30361 gcccgccgag cgccaccgtg tcgagcccga cgtggagcag gacctcgacc ccggcgtcgg
    30421 ccgcgatcgc gaccgcgtgc ccggaggggt ggaccgtcgc caccacgccg tcgcacggcg
    30481 acaggaggat cgccgagacg gggtcgatcg ccacgccgtc cccgaggagc cggtgcgcga
    30541 agaccgggtc gggcaccctc tcgatcggga cgaccggccc cgagagcggg gccttcagcg
    30601 ccagcgtcgg ctcgggggag gcggcccgag ggccgcctgt cggcggctcg ttcatggcac
    30661 ggggggcgag ggcagtatag gcgcggaggc cgggcggctc tcagccgtgc ccgttgccgg
    30721 agacgagacg gagctcggcg acggtccgcg cgagctcctc gacgatcggc gtcgaccccg
    30781 cctcccggaa cgcggcgacg agcgactcga ggtaccagag ggtctcgtcg cgtccggcgt
    30841 tgaaccgctc ccagagcgcg ggtccgtgga tccggaggtc ggtcaccatc gtccgggcgt
    30901 tgtggagctt gtcggccgcc gagacgagcc gcacgtgcgc cggctcgttc cggacgcggg
    30961 cgacgtacgc ctccttccgg agccgccacg gcggcttcgg cgagacgtcc gtgtcggtgc
    31021 agccgtcgac gatgtcggcg acgaggtccc cgaagcgctc gcggatccgc tccgccgtcg
    31081 ggcgcccgcc ctggtcctcc accgcgtcgt gcaggagcgc ggcgatcgcc tggtcctcgc
    31141 tgccgccgtg ctcgaggacg agcgccgcca ccgagaggag gtgcgagacg tacgggacac
    31201 cggtcccctt cctcggctgc tcgcggtgga gctccaccgc gaacgcgagc gcctcgtcga
    31261 acctcgacga caggatgggg ttcgcccggc ccggcatgga cggatggtgc cacgaatgcc
    31321 ccggacgacg ggagcgggtc gcggcgggag cggcgagcgc gtacccttgc cccatgtcgc
    31381 ccgctcgcaa ggctgccccg aagcgccccc gtccttccgc cgcgcccgtc ccctccgctc
    31441 ccgcggacgc ctccggcctg ggcgacgtcc cgcctccgct cgtctacgag cggaccgcgg
    31501 ccgccgccga ggacgagggg ttcgacgccg ccaccgccgc cgtcttcgcg gccgtcgccg
    31561 ccgtcgacgt cttctgcgtc tccgaaacga gcggaagcgt gcgtcaccgg ctcggctcgc
    31621 tcgtccacgc cgccgacctc gcggagcacg cgctcgagga cgtgggcgcc ggcgaggagg
    31681 agttcacgca cctcttcgcg ccgcgcctcc ccttcgacgt cctgctcgga gagaccgggc
    31741 gcctcacggc cgccgacctc ttcctcgagc gctggggcga cgagcttccg aaggacgccg
    31801 tccgggccgt gaaggcgctc gttcgcgccg aggacaccgc cgcccgcgcg acctgggccg
    31861 ggaaatccgc gacgatcctc gacctcgcga ccggccgcgc cctccccgga ccggctctcc
    31921 tccggcgcgg gacgcgtccg ttcgtctgcc gcctcgtccg cctcggcagc gccttcgttc
    31981 ccctcgccgt cgaaaggatc gtgaaccggg tcaccgagga agagctcctc gccgaggtcc
    32041 ggggggccgc gcagctcggc cgcgacgtcc tgaaggccgc cgggctccgg ctccggacca
    32101 cgaagaaggg gttcgggctc ggggcgcgga tgctcgcggc ggcggacgag atggggtgac
    32161 ctcgagagac ctgcgctgac ccagctgggt ccgggtcggc cgcgaccttc gagccgacgg
    32221 tcacggggct gcgccggcta tcatgccgtc gggtccaggt ctccctgttc actcgagggc
    32281 ccacgcaccg actccgatcg ggtcggccat ggagtggttg tatcgatgcg cgagccatgg
    32341 gccggtgagt cgcaaaccag caggcatcgg ctcctctccg agctgatcga agcgagccgc
    32401 gcccttcgcg gcgtcggagg catcgcgctt tcacccttcg cctttcaggg ggtcttcgtc
    32461 gtcctcgccg gcttcggctg gccccgcgcc ggagccgagc acaggctcct tcgcgacgcg
    32521 cccgttccgt ttctcgtcag ctcgctcctc gtttcggctc tcgcttccgc ggcgctcgtc
    32581 ctcgcatgcg cgggcgtttc cgctctctcg ggccgcaaga ccaggagggc cggcatcttc
    32641 ttcgtggcgc tcctcgcctc cctcccgatc ccgatgctcg ccttcttcgt cggctcgggg
    32701 accctcccga gcctggacct cgcccgagag gggctgccct ttctcctgcg ggacgccacg
    32761 ggctggggcc gggccgccgc cgtgaccctg gcctgcgttg ccgttccctg ggcgctcctg
    32821 agcgtgctca cgttgcgaag cgcctccgct ccgccgcgcg ggtctttcgc gcccctgccg
    32881 ctcgcgagcg cgggcctcct ccttctcgga gcggtcttcc tgctcttcgc gggggggaac
    32941 gtgctcgcgc acgtccatcc cggctcgcga cgcgcggcgg actcccggcg tcagctcctg
    33001 acgtgcctgt ttccctcgtt cccgctcctc ccggccctcg gggccgacga ggtcggtgcg
    33061 gaggcgctcg tggaccggct cggacgcgag ctgccgccgc ggagcgagct cgcgcgctac
    33121 ctctcctcgc tcgcatcgct cgaggcggcc ccccgtccga acgtcgtcgt cgtgatgctc
    33181 gaaggggtcc cggccggcca tgtcggcttc cgcggctatc gccgatcgac gacgccgaac
    33241 ctcgatcgcc tcgccgccgc cggggtctca ttcgaccgcg catgggccgc cgcgaacgag
    33301 tcgaagaacg ggcaaacggc gatcctggcg agcgtctacc ccatgcgggg ggccgagaac
    33361 aacttcgtct cgaacgccct cgactacccg cgcatcctct gctgggaggt cttccgcgcg
    33421 ctcggctacg cgaccgcctt catcagcacg caggacgaga cctggctcgg gatgcagaga
    33481 ttccagctgc tcgacggaag gccgccggac ctctacgtcc acgcgctgga catacccgaa
    33541 ggcgagacgt tccccgacag aaagaaggac gaggcgcccg tcgtccgacg cgccctggac
    33601 tggatgcggc gcgtggccga tccgcggaag ccgttcctcc tcgtcaccaa ttttcagcgg
    33661 acccacttcc cgtaccagct gccctcggga atcccgccga gcttccagcc gctcgccccg
    33721 ctcggaccca gattccggcg ctggaccgaa gcagacctgc cgaacggcgt gaacaatttc
    33781 gattccgcgt tgcgctacgt cgatgaccgg ctcggagagc tcctcgacgg gctccgggac
    33841 ctcggagtcg agaaggacac gatcgtcgcc atcgtctccg accacggaca gggcttcgaa
    33901 gcgggtctcg agcccgtctc gatgtcgctc gacgcgccgt acgtccacgt cccgctcgtc
    33961 ctcagctggc ccggaactct gccggcgggc cggatagagg agaacgtctc ggccatcgac
    34021 ctcttcccga cgctgctcgg gctgatcggg gctcccccgt gcccgggatg ggatggacgc
    34081 gcccgactcg gcggccctgc ttcgtcacgc ggacgctcgc gtcccatcta ctcggcgagc
    34141 atcgtctggg aggcacgctg gctcgtgcag ggcgcgcagg aaaaggtgct cgtcgacctc
    34201 atgacgggac gagcgaccgc gtttcgcggg gatgcttcgc ttcggtcgcc gaccgatcgg
    34261 ggctccttcg acgaggcgcc cgcgctcctt cggtcctttc tcgaggacta ttcgaggagg
    34321 gcgacgtact acacgtcacc ggacctgcgt cgctcgcggc tgtacgcgga gccggcggat
    34381 tccgatggag gcgctctctc ccgtacggat tgaggcgacc ctggaaggag tcgaccggcc
    34441 tggcgcgctc gccggggggc atcgcgggcg ccttctcctt ctccttgatc tccgagatca
    34501 tcacctcggt cgtcagcatg aggctcgcga tggacgaggc gttctggagc gccgacttcg
    34561 tcaccttggc cgggtcgatg atccccgcct cgagcaggtc ctccaccttc tcggtggccg
    34621 cgttgaagcc gatgtggccg cccttctcct cgagctcctc gaggatgacc gcgccctcga
    34681 tccccgcgtt cgcgatgatc tggcgggagg gctcctcgag cgcgcgcttg acgatgctca
    34741 tgccgacctg gatgtcgccg gactcctcga gcttctcgac cgcgtcctgc gcgcggagga
    34801 gcgccacgcc gccgggcggc atcgttctta ccgggtccgg cgactcgatc aggaggacct
    34861 gtttgtcgag cccgcgagcc gcacgatgtc ctcgttctcc agcaccgcct gcggcgcgcc
    34921 ctcccgcgcg acgtggagca tggcccgcgc cacctcttcg gtcgtcgtga cgtagcgggg
    34981 ggcgacccgg cgcaggagcg ggaagagcca gccggtggcc gcgtagaagg cccggtacga
    35041 ggccgtcttc gagcgctccc cgtgggcggg gcggatgaag gcgggccgga acatgtacgc
    35101 gccccggaac ggcagacgcc gcagggcgtt ctcggtctcg cccttgacgc gcgcccacat
    35161 gccggacccc ttcgcggtgg cgtcggtccc ggcgcccgag acgtagacga aggtcagggc
    35221 cgggtttcgc cgggcgaggg cgttcgccgc cgcgagcgtg aagtcgtacg tgacgcggcg
    35281 gtagtcggcc tcggacgagc cggccgccga gacgccgagg cagtagaagc aggcgtcgaa
    35341 ccccccgagc tcggcctcca cgccggagaa gtcggtgaag tcgcggtgca cgagctcgcg
    35401 gaggcgagga tgcgtgaggc ccgtgggcga ccgcccgacg gcgaggacga gctcgacgtc
    35461 gggagcccgc agggcctcgc gcaggacgcc ctgtccgacc atcccggtcg cgccgaagag
    35521 gacgaccttc acggcgtcag cccgccgtcg agacgctgac gtcgcgccac ttccggtcgg
    35581 actcggcgag cgcctcggcg gcgcgggaga cgtcctcgac ggcggcgacg tcggcgtacc
    35641 cggcgttgtt caccacgacg tcgatccggc caaacctctc gagcccggcc gcgaccgccc
    35701 tctgcgctcc ctcgaagtcg gtcacgtcga gggccagcgg caggacgcgg tcgccgtgcc
    35761 gtacgacgag atcggccagc tgctccggcc tgcgggcggt ggcggccacc gcatcgccgg
    35821 cgtcgagcgc ggcctcgacc gcggcgcggc cgaggccgcg ggaagagccg gtgacgaacc
    35881 agactttcga catcttcgct cctttcgggc gtgaggcggg cgcggcgaga agaggcggcc
    35941 ctgatacgcg aacccgcgtc tcccgggtcg tcccgggcgc gacggcgccc gcgactcgcc
    36001 ggactttaca tcttgatcct agtgtgtcaa gtgtgtagag tcgcccccgt gaaacgagcc
    36061 ccttcgccga ccccgcgtcc cgcccgttcg cggggaggct ccggccccgt gacgacctca
    36121 cccgattccg gcgcgcgccg gaccggcatc ctggaggcgg cggcgaaggt cttccttcgc
    36181 tacggctaca ggaagacctc gatggacgac ctggcccgcg cggccgggct ctcgcggcag
    36241 gggctctacc tgcacttcga gacgaaggag gcgctcttcc aggccgccat cctcggggtc
    36301 atcgagggcg ccagagccgc ttacctctcc gcgctggccc gcgaggagct ccctctcttc
    36361 gaccgggtgc tcggcgcctt cgaggccttt cacggctgcc acatcgggca gctcggcgag
    36421 cagtacgtcg gcgagatcat cgaggccgcc gcgagcctcc tcggcgacgc gcccgcggag
    36481 cacgaacgcc ggttcgtcgc ggacgtgacg gaggcgctcg cgggggaccc cggcctcggc
    36541 cgcacgggcc cggggatcgg accgcgggag ctggcggaga cgctctgcgc gacctcgtac
    36601 ggcctcaagc accgcgtcgc gacgcccgcc gagtatcgcg accggatggc cgtcgccctc
    36661 cgcatcgccc tccccggcgt cccgccggga cgcagggacg ccgcgagcgc ccgccccagg
    36721 aagcggtgag aggatgaaga ccgttctcgc cgtcggcatc cccgtcgccg tcctcgccct
    36781 gctcgccgcg tcgatctggt acgtcgccgg ccgcctccgg acgctcttcg ggctcgagcg
    36841 gcgctggccg ctgcggctcg gggtcgccgc cgcggtcgtc ggcgccgcgg tcgccatgct
    36901 cgggacggcg aggctcgccc acccgctcgc gggcgtggtc aacctcctgg gcggctacgc
    36961 cttcctcttc ttcgtcttcc tgctgctcgc gcttctcggc cttcacgcga tccagctccg
    37021 gtggacccgg ccccccgcgt ggagcggcgt ggccgccgtg tccctggcgg tcgcggcgac
    37081 gggagccggc gccctgcggg ccggctcctt cacggtcgcc gagaccgtga tccggctgcc
    37141 ccggctcgcg cgcgaggtca cggtgatgca gatctccgac gtgcacctcg gccaccaccg
    37201 cgggcgcgca tacctcgccc ggatcgtcga cgagacgaac cggcgcgctc ccgacctcgt
    37261 gctcgtcacc ggcgacctcg tcgactcctc ggcggcgctc gagcccggcg tcctcgagcc
    37321 gctcgccggg ctcacggcgc cggcgtattt cgtcggaggc aaccacgaga agtacgtcga
    37381 cccggagcgc gtgttcgcgc tggtcgcgcg aaacggggtc cgcgtcctgc acaacgaggc
    37441 cgtcgagacc cacgggctcc gcctcgtcgg cctcgactac atgaacgcgg acgaggacgc
    37501 cttcgacatg cacccgtccg acgacgcgcg cacgatccgg tcggtcctgc ccggcctcgc
    37561 ctcgaggaac ggcctgccgt cggtgctgat gcaccacagc ccggtcggcg cctcgtacgc
    37621 ggcggagcag ggcatcgacc tgatggtctc gggccacacg cacgccggcc aggtcttccc
    37681 gttcaccctg ctttcggggc tggcgttccc gttcaaccgg gggctgcact acgagggggc
    37741 gaccgccgtc ttcgtttcgc agggggccgg caccttcatg tcgcgtgcgc ggctcggcac
    37801 ctccaacgag atcaacctgc tgcgcctggt gcccggagcg tgacccttcc gggtctcgcc
    37861 gattccgcct gacgaccggc cggccgcgtg cgggatggaa ccgcgggcgc agtcgcgaga
    37921 cgacggccgg cgcgcccgct ttcgcgctgg accgtgtcga cccgcggaac gtccgcaacg
    37981 actcgctccc gccgcggcac agagaagccg ccccaggcgt gaaacggccc ggcttcgcga
    38041 gaagccgggc cgtcgggtct tccgtgtcta cctgggcatc cctcacgcgg agggggtccg
    38101 ggggaatccc gctcggcggg tcacccggtt cactcccccg tgcggagggg gcccggggga
    38161 atcccgctcg gcgggttccc ccggttcaag atcagtacat cccgcccatg ccgccgccgg
    38221 gaggcatcgc gggcgccttc tccttctcct tgatctccga gatcatcgcc tcggtcgtca
    38281 gcatgaggct cgcgatggag gaggcgttct gcagcgccga cttcgtcacc ttggccgggt
    38341 cgatgatccc cgccttgagc aggtcct
//
LOCUS       DAOONL010000027        35703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_947_length_35703_cov_17.984375, whole genome shotgun sequence.
ACCESSION   DAOONL010000027 DAOONL010000000
VERSION     DAOONL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 35703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35703
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_35703_cov_17.984375"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /gene="sufC"
                     /locus_tag="PLP50_06295"
     CDS             <1..392
                     /gene="sufC"
                     /locus_tag="PLP50_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141371.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPA51197.1"
                     /translation="ARLVEMEEALLSRNVNEGFSGGEKKRNEIFQMAVLDPSLAILDE
                     TDSGLDIDALRVVASGVNSLRSPERSMLVITHYQRLLNYIVPDVIHVLSEGRIVRTGD
                     KSLALLLEEKGYSWLEEEAPVGSGTAS"
     gene            389..1783
                     /gene="sufD"
                     /locus_tag="PLP50_06300"
     CDS             389..1783
                     /gene="sufD"
                     /locus_tag="PLP50_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPA51198.1"
                     /translation="MSATAPAPTVSEAIAAATAAALAGSRALDERGAEALPLFARNLR
                     RDGQEALGRLGLPRPADEEWRYTSVAALARRLPDLLPASAARGGSPTVSAAAREALSC
                     FGPLSAERKGLHVLVLVNGRLDPDLSHVDGLPAGVRFEALSRAFAAPPALLEQRLGLA
                     GSHRSHPFAALNAAFLSDGAFVEVPDGVVLAEPLVVLHVADAAAGPLAAHPRVVVSLG
                     ENAQASVVEAYCGAGEAFVNAVTEIYLLAHARLDHSKLQEESPEAFHVGTAEAHLGPG
                     AQLYSRVLSVGGRIARNELTVRLHGDGAGATLDGLFLARGTQEVDNHTLVDHARPHGG
                     SQQRYKGILSGTARGAFDGKVVVRPAGAFTDAHQSNRNLLLSDTARVDAKPQLEIYNN
                     DVKCTHGSATGRLDADALFYLRSRGFSESEARSVLTLAFASEIVDRVPLDPLRAHLTE
                     RVLGWLSGDAPGAR"
     gene            1780..3027
                     /locus_tag="PLP50_06305"
     CDS             1780..3027
                     /locus_tag="PLP50_06305"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPA51199.1"
                     /translation="MIDITLTPATFDVETVRADFPILSRKVRGRPLVYLDSANTTQKP
                     EAVIRASDRFYRETNANIHRSTYRLSEEATAAYEGAREKVRRFLNAASTREVVFTRGT
                     TEAINLLASSFGQAFVKAGDEVLITGMEHHSNIVPWQLLCERTGARLRVLPIDDRGEL
                     VLEELDALLTERTRILGVVHVSNALGTVNPVKEIVARAHAKGVPVLVDAAQSLPHLGV
                     DVADLGCDFLAFSGHKVYGPTGIGVLWGKEEWLEKLPPYQGGGDMIASVSFEKTTYAE
                     LPARLEAGTGNLAGGVALGTALDYVTTIGLERIAAHERALLAHATERLSEVPGLRIVG
                     TAREKAGVVSFVLDDVHPHDVGTILDQEGICIRTGHHCAQPVMARYDLPATARASFGL
                     YNTLEEVDALVDGLRRVREVFGA"
     gene            3024..3473
                     /locus_tag="PLP50_06310"
     CDS             3024..3473
                     /locus_tag="PLP50_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPA51200.1"
                     /translation="MTALTDLYQEVILDHNRSPRNFRAIEGATHSGLGHNPLCGDQLT
                     LYVRVEDGTIREIGFQGKGCAISKASASLMTEAVKGKTLAEAEELFGRFHDLLTGPSD
                     VPAEAKSFGKLAVFSGVREFPVRVKCATLAWHTLHSALAGGSPASTE"
     gene            3526..3819
                     /locus_tag="PLP50_06315"
                     /pseudo
     CDS             3526..3819
                     /locus_tag="PLP50_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
     gene            complement(3845..4639)
                     /locus_tag="PLP50_06320"
     CDS             complement(3845..4639)
                     /locus_tag="PLP50_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51201.1"
                     /translation="MSGATLAAARAPLPLARVLRAYLQEARFEVLAALRTTGLALPFI
                     AIPVAIYLLFGVVISGSAGGGELGPGIHNYLFVGFAVLAVAMPGVFTGVTVATEREGN
                     LLKLKRALPLPPGATIVAKVAMAMVVAGIALTLVVVAALLAAKITLSPGQLLIVWAVL
                     FAGTIPFSAIGLLLGTFGSASAAPAWGNLVFLPMMWLSGLFIPLPDSLKRWVIVWPTF
                     HLDQLALGLAGVRQFTYIPPELAAAVLTGVTVLCGGLAIRRLARRG"
     gene            complement(4636..5499)
                     /locus_tag="PLP50_06325"
     CDS             complement(4636..5499)
                     /locus_tag="PLP50_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51202.1"
                     /translation="MNVTKRYGTTVALDGLHLEVRPGELLAVLGPNGAGKSTAIGLWL
                     GTLQPDEGTALVMGGPPLEAASRLGVGVMMQEVALAPTLTASEHVALTSSCYRDPLSV
                     EEALDLTGTRELARRRYGRLSAGQKRQVQFATAVCGRPRLLFLDEPTVGLDVPAREAM
                     WKAIAKLRNDGCSVVLTTHYLEEAEALADRVVVLARGRIVAEGSVDEMRALVTRKRIR
                     CASSVDVGAMRSWPGVVEATRDADLVNVTASDAETVVRRLLAEDPDLRQLEVQQASLA
                     EAFTEITREAA"
     gene            complement(5701..6789)
                     /locus_tag="PLP50_06330"
                     /pseudo
     CDS             complement(5701..6789)
                     /locus_tag="PLP50_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(6800..7489)
                     /locus_tag="PLP50_06335"
     CDS             complement(6800..7489)
                     /locus_tag="PLP50_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51203.1"
                     /translation="MTGSRGSLTARLIARDLHHYRWLILGTAIAGAASLVLTRFGDGD
                     GISTGPNVGFLLLLTTVIVLGVALPMLVLKEHETRSDLFVLSLPVSAAQYSFAKVASA
                     LAAFLLPWTALTGGAAAVTALSPAPDGSLPFFVATMTFLLVNFCLLMALTVITRSEPA
                     AIAGILVTNFSVTVFMSRLARLPGVAGRSKDAVATWSPEVLAVLAVELVVILLSLALA
                     FYVPSRRRDAL"
     gene            complement(7486..8394)
                     /locus_tag="PLP50_06340"
     CDS             complement(7486..8394)
                     /locus_tag="PLP50_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51204.1"
                     /translation="MTTTATPAIALRGVSKRYRFFALDDVSFRLEPGQILGFVGPNGA
                     GKSTTIRILMALVRPDAGDVEVLGCRMPDEQVRAKREVGFVSEEMRLFPGATIGWHVG
                     FVRSVFPGAWDDRYAARLLQRFHLHREQRVKGLSHGEQVKAALLLALARRPRLLVLDE
                     PTVGLDPVARQEVIGELMEVVRDEERSILFSSHNTLDVEQICDQIAFIDRGRIVDSGD
                     KEAFLERWRRLSLELPATIALPEVPGVVDVARSARTAVVTVNAFEEALPAAYQRAGAV
                     VRDVQRMTLEEIFVATVMNRRKERGE"
     gene            complement(8391..8780)
                     /locus_tag="PLP50_06345"
     CDS             complement(8391..8780)
                     /locus_tag="PLP50_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPA51205.1"
                     /translation="MHAELTISQADPRPMYQQIIEQVCRRIAAGDWEPGQEIPSIRAL
                     AASTRVSVITVKRAYLELEHQGVLTTRQGRGTFVAENVKLQQDLREQELDGHLARAVD
                     VGRELGLGTRELEQRLRKVVRQREEDR"
     gene            8934..9686
                     /locus_tag="PLP50_06350"
     CDS             8934..9686
                     /locus_tag="PLP50_06350"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPA51206.1"
                     /translation="MNLILVLPDELEADGTVRLTGRRARHVHDVHRARVGDELVVGLR
                     NGATGRGTVLAVSADEVRLAVELVGEPPPPSGVDLLLAMPRPKVLRRALQAAASLGAK
                     RIVLVNAARVEKSYFDTPFLQPEEIERNLVLGLEQARDTLLPELLVRPRFRPFVEDEL
                     DTLWPSDSFGRLLAHPADAPALGAPDAPRVGPRSVVAIGPEGGWVPFETDLLEERGFA
                     RFTLGPRVLRVETAVAFAFGQLLLSRALQSGR"
     gene            9724..11766
                     /locus_tag="PLP50_06355"
     CDS             9724..11766
                     /locus_tag="PLP50_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPA51207.1"
                     /translation="MRLEYGLALGLLLAVPAFAQDGPPPVPAAPISAPSPGAPYRDTV
                     LSGRTTNAAGLSFTVGAARYEVATGSLWEVTTKDGTPVGAFFRGAGTLAFSAGDPQAA
                     RLAARNAKHVGGAKEVDGELRAAFSRATFLFSPALRPAWSYAAGEEPPVRVFATHLER
                     FEHDRTPQVASRLAIAEAAQGAFFAATLEADPDLRHVYDPVTDDEEVLRVVDRPAGLP
                     TGFPEMRFARDLSRRPLGRTRRQAPRVDVRLVAVDVDVREGTAPWGELKVTETFVAVR
                     PVSFLVLDFATETIYRNDLLETRLRALTDGEGRPLPWALGDGELVVALPKALAPGARF
                     TLRLDYEAPYFERAGGDNLWELPIASGWYPQPPFFNASHHTFHAVVRARKPLLPFASG
                     DTVRRSEEDGWNVLETRLERPVPFATVLAGKYTTQESTEDGVTCRVASYGIPKEMSGK
                     MLLSVFHGVRKFYEWLLGPFPWKEFTIVEINAYGFGQAPPGMMRITKEAFQSSIFTDE
                     ISSLFSHGINQRVAHEIAHAYFGYVVADASPQHQWISEAFSEIASMYAIEKLKGKAEG
                     KKLAGTWAGAARHSAKAAPIDLANDLAPKIASTWDSSTAIDRAELVYSKGAHLLQTLR
                     LELGDDLFFTVLRSFLRSFEKQRDVTTDDFVALLSFATKKDWKPWFEKYYYGTEMP"
     gene            complement(11782..14217)
                     /locus_tag="PLP50_06360"
     CDS             complement(11782..14217)
                     /locus_tag="PLP50_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA51208.1"
                     /translation="MVRRSLVPTLALFPFLAVAATAAAQENVAFRTPPEEIRKLVDAP
                     LPPSVLVDPAGARLVLLDLPGYKTLAEVAEPELRLAGLRINPKSHNRARLNVTTGISV
                     KEIASGRSARVEGLPAAPRIQWTRFSPKGTYFSFVQCDADGLSLWVVDLASARASRVT
                     PASVSAVLDFPYQWLPDESGLLVHVRPSLEPFTPSAELPAGPVVKVAAGRKAPARTWQ
                     DLLKSENDEKTFAHYATTEVRRFALDGTSAAILPPAIRRSVRPSPDGKWILATTIVPP
                     FSYRFPVYRFGYRVDVLDAAGKLAATLVEKPVQDAIPVAFDATEAGRREFDWRDDAPA
                     TIVFAEAQDGGDPANDVPFRDRLFLLDAPFDGAPRPFAKTRNRFSEVTWGAGGVAVLS
                     DFRWKDRNTKTYLAMADAGDSEPKVLFDLSSENLYALPGDFVTRVDGRGRPLLLTSRD
                     GKRLFLSGEGYSPEGNRPFLDAYDVATGKTTRLWRADGTSTYERIVRVLDAERGVLLT
                     RVEGSKLFPNYQVRNVKARIAPRPVTSFENPYKPLEAVATRKVRYRRADGIDLAGDLH
                     LPPGYDPKRDGRLPLLLEAYPTEYKDKAAAGMVDSSPHAFVRPYWGSPVFWALRGYAV
                     LENAQFPIVGEGEKEPNDTFVEQLVANAKAAIDALEKEGIVDPKRCAVTGHSYGAFMT
                     ANLLAHSDLFAAGIARSGAYNRSLTPFGFQAEERTYWQARDVYSRMSPFDHAATLDEP
                     ILLIHGDADNNPGTFTLQSERLFEAIQGLGGRSRLVLLPFESHGYAARENILHMLWET
                     DTWLETWVKGKKD"
     gene            complement(14268..14690)
                     /locus_tag="PLP50_06365"
     CDS             complement(14268..14690)
                     /locus_tag="PLP50_06365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPA51209.1"
                     /translation="MAVDIDILYTGGLHCEATHRPSGSKLVTDAPLDNGGKGEAFSPT
                     DLCATSLGSCLVTTMALVATSHGIELAGTTVHVVKEMVADPRRRIASLPTTVTFPPSL
                     ASLPAEKRALLEATARACPVTQSLGPNVEVPMTFVWPA"
     gene            complement(14754..15218)
                     /locus_tag="PLP50_06370"
     CDS             complement(14754..15218)
                     /locus_tag="PLP50_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51210.1"
                     /translation="MVRPRPLPEKARWLVLVAGLTASIPLYAVVVFVLATTGAPGASA
                     PIGLRYAVYGAAFGAALLALVLAPRVGVDVATSPAYPEFLRKTLVSLAVAEVSAILGV
                     VLFVLGRNLGDFLVLAGLALAVNLLGIVPRGLRWFRLHEEPGSGGAPPLGPG"
     gene            15386..16852
                     /locus_tag="PLP50_06375"
     CDS             15386..16852
                     /locus_tag="PLP50_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HPA51211.1"
                     /translation="MEIDRESCLAAFLGRDRRFAGRFYAGVTSTGVYCRPGCPAPLPR
                     PSNVRFYPCAAAAEEAGFRACRRCRPETAPGTPAWAGTSTTVARALRQIESGLVDHAP
                     LGVLAARLGVGERHLRRLFAEHLGASPVAVAKTRRLHFARRLLDETPLRMSDVALAAG
                     FSSVRRFNEAMRAAFGAPPTVLRRAFAGSPASAAGVTLRLPYRAPFDSVELFAFLGAR
                     AIPGVESVEGSLWRRTAELAGVAGTVEAEVDAARSSIVVRLFGLPPGAALDAARRAGR
                     LFDTDADPAAIAAHLGRDPLLAPLVELRPGLRVPGAWDPFETAVRTVLGQQVSVKAAR
                     TLAARLVSRFGRPLPPGVARGSLTHLFPRPDDLAEADVASIGLPSRRGETIRVLAREV
                     AAGRLDLGPAASLEEAERRLAAIPGVGPWTAQAIALRSLGEPDAFPGGDLGVRKALAV
                     RGVPPTTAAAEARAAAWRPWRAYALVHLWTDLASREDS"
     gene            16849..17358
                     /locus_tag="PLP50_06380"
     CDS             16849..17358
                     /locus_tag="PLP50_06380"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPA51212.1"
                     /translation="MTTTLETATIDTPAGGFWLAARDGALVAAEFAERVPHRLSRLEA
                     RFGPLELRDAADPAGAASALRRFLSGDLNALEAVPLDAGGTPFQRDVWAALREIPAGT
                     TATYADLAARVGRPAAVRAVGGANGANPVAVFVPCHRVVGKDGLRGYAGGLERKEWLL
                     AHEATATRT"
     gene            complement(17411..17944)
                     /locus_tag="PLP50_06385"
     CDS             complement(17411..17944)
                     /locus_tag="PLP50_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA51213.1"
                     /translation="MKNRIEKRLDDLDRWKDAFLGALSATSPERLAFRADGVGWNALD
                     VVQHLVLVEEGVLAYARKKLLAPPQPISIVDRGKLALLVGVLRSPIRVRAPVAQVVPS
                     ETLPLETSATRWDQSRRDLRTLLLGLPEERRAALFFRHPAAGGLDPAGTLIFLHEHAR
                     HHEAQVRRIGRAPGAPR"
     gene            complement(17997..20483)
                     /gene="lon"
                     /locus_tag="PLP50_06390"
     CDS             complement(17997..20483)
                     /gene="lon"
                     /locus_tag="PLP50_06390"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPA51214.1"
                     /translation="MHPSKVLPVIPLRNAVLFPGVSFPIAAGRPGTLHAIETAMKSPE
                     QLVFAVTQRQDLEAVTPELLHTVGTIASIGPIQRGVGGARLLLQGKARGIAMRIEEKE
                     GRLEAVVVEAEEMPPLDSADTAFVALLKELRERSAELAKKLGLPDEAVGNVLTEADDP
                     GRLADLVAGYIDIPTAERQLLLETLPVEDRVRRTLVHVQRQIDVLDAQEEIKSKVQEE
                     LGDRQREMFLRQQLKAIQKELGEGEDDGDDLKGLKEKLDALVLTEEVRKEIDREWKKL
                     QRLSRESMEAQVVRNYLELVAELPWSARSEEHLDVVEAQRILDEDHYALKDVKDRILE
                     FLAVRQLLGRKTEAAAAPSADGAPADAPAGAGVPAPTPAGEKQPNRGRILLFAGPPGV
                     GKTSIAKSIARALGRKYVRISLGGARDEADIRGHRRTYVGAMPGRILQGMRQAGTKNP
                     VFLLDEVDKLGVSFQGDPASALLEVLDPAQNDSFTDHYLNVPFDLSEVLFVATANFVQ
                     SIPGPLLDRLEVIEFSGYTEREKTLIARNYLLPRQLSENGLGAGQLEVTDAALAEIIS
                     SYTREAGVRQLERELGKLGRKVARRIAAGEIEKAAVDAADVAPILGRKKIQPEKIVHE
                     DQVGVATGMYYTPAGGDIMFIEAALMRGKGEVLLTGQLGDVMKESARAAWTFARSHAA
                     LLGIDDTSFERDLHIHVPAGAIPKDGPSAGVAMASALVSALSKRPCRRDVAMTGEVTL
                     SGRVLPIGGVKEKLLGAVRAGIRTVVLPKENVPDLEDLPQDVRDGLEIVPVQDLSEAL
                     AVSLRGRVSGGQLKFAEEPAGTGTGAPPTH"
     gene            complement(20688..21065)
                     /locus_tag="PLP50_06395"
     CDS             complement(20688..21065)
                     /locus_tag="PLP50_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPA51215.1"
                     /translation="MRIERTSSFRTTVERSRFFAVAFPAAAEADVLDAVKAARGEHRK
                     ANHHCWAFRGRDAAGALVEKSKDDGEVGHPGRLLLELLKRDDLEGGLLVSRIFGGVKL
                     GVGGVSRAFREAAEGALEGVRSP"
     gene            21321..23561
                     /locus_tag="PLP50_06400"
     CDS             21321..23561
                     /locus_tag="PLP50_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51216.1"
                     /translation="MHSHLPTRPGSRLSVSLVVFLAVFALAAHGSAGTATVTGNVTDP
                     FGAPVPEARVTVSAEDGGVRAVATTSLDGAFRVAELAGGRYTITAEAAGFAPYAKAGV
                     ALGADRETRLDVRLSLTRFEGSATVEAEPGNFLIEEKEVSLLKPLDKAGLLSHESMVA
                     VGGGSSVAQKIYVRGFEDTLLNVTVDGAAQAGELYHHQGRVQIEPEFLKAIELEAGAG
                     PATSGAGALTGALRLTSKDAFDHLPPGRKVGALVKATGELNGDDAFGGVVSLYGKLSQ
                     NLGLVVSGVARSGNDYADGNGDVAAPTAYDQKRGHAKLTGLFDAHRFDLSYERLTDEG
                     TYYERPNLTNFTGTYVLSDHEMSRDTVSYNHRFDPAGDALDLAGTAYWTRSEFHNART
                     TTGALYGAGEFASFGLDLRNTARFGRHVLVGGIEARRDDASGTQQATPPRFWGSSDQE
                     ASVLGLYLQDHWQIHPIVALSAGLRWDSYRHEVVAGVGAGARNDDSGFSPNVSVSVTP
                     TTWLTLRGSYARAFRGITIREAFFSGLYVHRGGLDPERADNLEAGFDVRRGPWFVRGT
                     LFRQNIHDYINAVYEGVTAWGYWENVGDAKVEGYEVETGARWGSLSVAAGVWDSKNTI
                     AGRPMTDADLGLGTSIGRTWTLRADWSAPGPRLDLGLRVRLVEDEPNAIAPTAPDKDG
                     YAVTDLHTSWEPIDGLGLTVGASVTNLFDAYYYDQATYTTNAAGAKLGFAAKGRAFVL
                     STAFRF"
     gene            23584..>24622
                     /locus_tag="PLP50_06405"
     CDS             23584..>24622
                     /locus_tag="PLP50_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPA51217.1"
                     /translation="MTAAHRPRASGRAREVVFWVHLASGLVAGLVVAVMSATGVAIAF
                     EHEILAFFDRRVARVAAPEGARRLSLDALVSSAAEKRPGFRPTLFLVPSDPTSALELR
                     AGREGALYVDPYSGSVSDPRSGAAHEILHTLEEWHRWLGLEGRGQAVGKLVTGVCNAA
                     LVLLCLTGLWVWLPLLPAGRSAWRRRLWFRRGLEGPARDRSWHDVVGFWTLPVLALLA
                     GTAVVFSFEWGHRLVFAAFGEEAPAARGPGMLAGPPVSLPDGPPGRAPLSLEAIHAAA
                     AARHPGHEAIALPLARTAGPVRPLDVVVFEPAPFQTRGRVQLKVDPFTGDVLSTFGWA
                     DRSAGTRARVLI"
     assembly_gap    24623..24722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24723..24959
                     /locus_tag="PLP50_06410"
     CDS             <24723..24959
                     /locus_tag="PLP50_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HPA51218.1"
                     /translation="STFGWADRSAGTRARVLIRFLHTGEALGWPGRIVAVAASALSLV
                     LVYTGFTLSWARFRRWRARRRRDLPASEEVRAAF"
     gene            24988..26607
                     /locus_tag="PLP50_06415"
     CDS             24988..26607
                     /locus_tag="PLP50_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPA51219.1"
                     /translation="MPGLFVAQRAGRLRLVAALLTALVSAPSFAGEPVAPPCGTAAEL
                     KAALARAADEAARARIANGFADCAARATTPLLSPGRSPGSVRALFVVRSNAEKVFLAG
                     DMNGWSTSAQPFERLAGTDLHLLEVEVPDGARLDYKLVVSGTEWTLDPWNPRTMTGGY
                     GPNSELRTPAYVPPAQVEPGTPTANGSWAELKIESRAMNGPRTAFVWSSAPAGATGPY
                     PVLYLLDGTDYRDFAKAHVVAGNLVGAGRLPPLVLVLVPPVDRRAEYDDDRSAPDGGG
                     ANGPRGREAPGVAPPHAAFTRFLVDELVPAVEARWPVRKDAAGRGVMGASLGGFAALS
                     VTARRPGVFGRCGAQSTGGGDGGGFEALLADLGSLPAASVRVHLDAGTFEALFHGFDL
                     LSLTRRLRTALSQRQVVQYREVPEGHSWGSWRARLADALTFFWGDERPAPSPASRPLA
                     GPASGAARFSGEPVSLDVKDADLADVVRTLTDGRGLSVVAPPSFRGTVTASLRDVPWD
                     QALDLVLAGNGWGFVREGNVIRIVRRGDPGH"
     gene            complement(26624..28708)
                     /locus_tag="PLP50_06420"
     CDS             complement(26624..28708)
                     /locus_tag="PLP50_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPA51220.1"
                     /translation="MAEPKPEASPAAFEDVRLPDGAFRTLAPGETYEPVVPAGASVPE
                     VTVRSVAQGFLWSVVFSAAATYIALKLGQGIESTIPISILAVGASVLFVKLLAARAST
                     LLENVNVLAVGATSGIIAGGSVFTMPAIHILGLEGRSSFWQIFLVPLLGAVLGVFLLA
                     PFRRYFVRDLHGKLPYPEARATTEILVAGKRGGRSAVVLSWSAALAAVFDFVGPSMKG
                     WAENFSTAAIPALSGFTDRFKAVFTMNTSAAVFGLGYIMGLDYAAIIMAGSMVSFLVL
                     VPLFAWLSQWVPGAISAGAPALAAMPADDIFFEYVRPIGIGGIFCAGLLSILKMSPVI
                     VQATRQAFGEVARLARGGGAAAEAERTDRSLPMWVVLAGIVATALAILLYFRLSVLAG
                     TPGASRLALVSTGLTLLIAFLFAAVSAWAVAMISITPVSGMTLTTLIVTAVVLSSLGL
                     SGESGMLQTLLVGGVVCTALSMSASLVTQYKIGYWLGATPRRIEVWNLLGSVAASAAT
                     TAVILLMAKVYGFAPSPLHENPLPAPQPNAMAAVLRGVMGDAGAPWFLYAVGAVFAVV
                     VEMCGVSGLAFALGMYLPMELNSPLVLGAAVAWLVKRSTPDEPLAKARHEKGTLIASG
                     FIAGGALVGVLAALLKFVEDSTGRTLVPDLTAVPGVGAWLSAWGNWLGLAVFLALAAF
                     VYLSSKRETVEG"
     gene            28773..29402
                     /locus_tag="PLP50_06425"
     CDS             28773..29402
                     /locus_tag="PLP50_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPA51221.1"
                     /translation="MPSENAPTRREPPVLLALALAALVVSGVGPKDRFTWLLEVAPVL
                     IGAPLLVATTRRFPLTPLLYRLLFLHALVLMLGGHYTYAQVPLGEWAKEAFGFARNHY
                     DRLGHLMQGFVPAILAREVYLRRSPLRPGGWLRLAVTSVCLAFSALYELLEWAAAIAT
                     GEAATAFLGTQGDVWDTQWDMFLALCGALLAQALLARLHDRQLARLATA"
     gene            complement(29474..29803)
                     /locus_tag="PLP50_06430"
     CDS             complement(29474..29803)
                     /locus_tag="PLP50_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51222.1"
                     /translation="MKKTTAVLLVAVLAIVLGSTAVATMDMQKDMNAKYPTAKAKCAS
                     CHVKAMAKKGDAEVNAFGKDMMKFVVDPKAEKKTFDWAKLEPLDSDGDGKSNLDEFKA
                     GTNPGAK"
     gene            complement(30020..30238)
                     /locus_tag="PLP50_06435"
                     /pseudo
     CDS             complement(30020..30238)
                     /locus_tag="PLP50_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
     gene            30553..30966
                     /locus_tag="PLP50_06440"
     CDS             30553..30966
                     /locus_tag="PLP50_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51223.1"
                     /translation="MRVPTRFLLVALVALVLAPALVAQEGGRIDTAIASDSRETMAER
                     SSFPPTQPKIYVFAMVADAPPDTPVRAVWIAEKCEGQPPNTKFAERTTKFPGGATWIP
                     FSYTKPNPQWASGAYRVELYLGKQLAATVKFSVTK"
     gene            complement(30956..31579)
                     /locus_tag="PLP50_06445"
     CDS             complement(30956..31579)
                     /locus_tag="PLP50_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPA51224.1"
                     /translation="MRVLLRYFLQGLLVFVPSVLTIAIVVFVFRAVDAWMGLPVPGAG
                     FLATIALIVVVGALASNFVTKRVFELVEGVFRRLPFVKLLYSAIRDVTDAFVGERKGF
                     ERPVFVTLDEASGVKAVGFLTRDSLDGAGLADHVGVYLPQSYNFAGQLLVVPRRLVTP
                     VTARGADAMAFIVSGGAAELRGGAQSEATAPEGRPHADTGMPGGATS"
     gene            31740..33521
                     /locus_tag="PLP50_06450"
     CDS             31740..33521
                     /locus_tag="PLP50_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA51225.1"
                     /translation="MLLRRVGKTPDREAYLRPVPDGWEALSWREVGDRVRAIACGLRA
                     LGLEDEERASILASTRLEWILADLGILSAGGATTTIYPSTTTPDSLFILQDSDTRFLF
                     AENPEQAERIARERSKVPGLKAIVLLEGEAAGDGFVLTTEDLMEKGRAWDAAHPGRFE
                     ALVEAVRPDALATLMYTSGTTGRPKGVELTHDCWVYEAEGIDALGLLTEDDRQFLWLP
                     LAHSFGKVLEAAQLRIGFSTAVDGRVEKIVENLGQVRPTFVAAVPRIFEKIYNKIVGG
                     AKEAGGAKWAIFSWAVGVGREVSKLRLAGKEPAGLLALKHKIAHKLVFSKLQAIFGGR
                     LRFFISGSAPLARALGEFFHAAGVLIAEGYGLTETSAASCVNTFEHLRFGTVGHPLPG
                     TQVRIAEDGEILIKGRGVMRGYHNLPEQTAEALDADGWLHTGDIGHVEDGFVKITDRK
                     KDLIKTSAGKYVAPQMLERKLAVACPYVSHSLAHGDNRNFVSMLVALDPEAITKWAAA
                     KGLGGRPYAEVVASKEVRDLIAPYIDEVNADVASWEQVKKWTILPADLTLETGDLTPS
                     MKLKRKDVERKYRQTLDGFYEGTVVDV"
     gene            complement(33567..>35703)
                     /locus_tag="PLP50_06455"
     CDS             complement(33567..>35703)
                     /locus_tag="PLP50_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPA51226.1"
                     /translation="PVEIFRNLLVGAEGTLAFISEAVLSTVPDLPVKYTGLLLFPSMH
                     AACAAIVPLRDSGAKALELLDRASLRSVESQPGVPPSIRTLPEGAAALLVEYQAGDEA
                     ERKGLEAVAAAAVGPLALLEPARFTHDATEQALLWKVRQGTFPSVGAARRSGTTVLIE
                     DVAFPIEHLADAAVDLTKLFVKHGYPEAILFGHAKDGNLHFVITQSFNDQAAVEKYAR
                     LIEDVVELVVKKYDGALKAEHGTGRNMAPFVETEWGPEAYSVMKRLKALCDPEGILNP
                     GVILNADPLCHLKDVKPMPTVEEEVDKCIECGYCEPKCPSRELTLTPRQRIVLRREMT
                     RLEAEGTNAPLLAALESEFPYAGVDTCAVDGLCATACPVSIDTGQLTKRLRKARHSAR
                     ARKAALAIARRFATVEPLLRTGLRAGHLVQSALGAGAMAGITRAIRAVVGKPFPQWSA
                     EMPRAAKAARPATTKAGAAAVYFPACISRTMGALPGEPSETSLMEAFVTVAQRAGTPV
                     FIPEDVEGVCCGVPFSSKGYTEANAFAANRAIERFWAWSDEGRLPVVVDTSPCTYGVK
                     GSRPNLTPENQARFDRLTILDGVAFTHDTLLPKLTAKRKAGPVALHPVCSLTKMNLGA
                     KLEGIARFCSDDVTVPLSAGCCAFAGDRGFLHPELTASATKHEAAEVKAKGCEEHLSS
                     SRTCEIGMTRATGEVYRSYVFLLEKVTRG"
BASE COUNT         5095 a        13018 c        12573 g         4917 t
ORIGIN      
        1 aggcccgcct cgtcgagatg gaggaggcgc tcctctcgcg caacgtgaac gagggcttct
       61 cgggcggcga gaagaagcgc aacgagatct tccagatggc ggtcctcgac ccgtctctcg
      121 cgatcctcga cgagaccgac tccgggctcg acatcgacgc cctgcgcgtc gtggcgtcgg
      181 gcgtgaactc cctccggtct cccgagaggt cgatgctcgt catcacgcac taccagaggc
      241 tcctcaacta catcgtcccc gacgtcatcc acgtcctctc cgaaggccgg atcgtccgca
      301 cgggcgacaa gtcgctcgcg ctcctcctcg aggagaaggg ctactcctgg ctcgaggagg
      361 aggcgccggt cggaagcggg acggcttctt gagcgcgacg gcccccgccc cgacggtctc
      421 cgaggcgatc gccgcggcga cggccgcggc cctcgccggg tcccgtgcgc tcgacgagcg
      481 tggcgcggag gcgctcccgc tcttcgcgcg gaacctccgc cgcgacgggc aggaggccct
      541 cggccgcctc ggcctccccc gccccgcgga cgaggagtgg cggtacacca gcgtcgcagc
      601 gctcgcccgg cgcctgccgg acctcctccc ggcctccgcg gcgcgcggcg gctcgccgac
      661 ggtctccgca gcggctcgcg aggcgctctc ctgtttcggc ccgctctccg cggagcggaa
      721 ggggctccac gtcctcgtcc tcgtgaacgg gcggctcgac ccggacctct cccacgtcga
      781 cggcctcccc gccggcgtcc gcttcgaggc cctctcccgg gccttcgcgg cgccccccgc
      841 cctcctcgag cagcggctcg gcctcgccgg ctcgcaccgg agccacccgt tcgcggcgct
      901 gaacgcggcg ttcctctcgg acggcgcctt cgtcgaggtc ccggacggcg tcgtcctcgc
      961 tgagccgctc gtcgtcctcc acgtcgccga cgccgcggcc ggtcctctcg ccgcccaccc
     1021 gcgggtcgtg gtctcgctcg gcgagaacgc gcaggcctcc gtcgtcgagg cctactgcgg
     1081 cgccggggag gcgttcgtga acgccgttac cgagatctac cttctcgcgc acgcccgcct
     1141 cgaccactcg aagctgcagg aggagtcgcc ggaggcgttc cacgtcggca cggccgaagc
     1201 gcacctcggg ccgggggccc agctctactc gcgggtcctg tcggtcggcg ggcggatcgc
     1261 ccgcaacgag ctgaccgtcc ggctccacgg cgacggggcc ggcgcgaccc tcgacgggct
     1321 cttcctcgcg cgggggacgc aggaggtcga caaccacacg ctcgtcgacc acgcccggcc
     1381 ccacggaggg agccagcagc gctacaaggg gatcctctcc gggacggcgc gcggcgcgtt
     1441 cgacgggaag gtcgtcgtcc gccccgccgg agcgttcacc gacgcccacc agtcgaaccg
     1501 gaacctcctc ctctccgaca cggcgcgcgt cgacgcgaag ccccagctcg agatctacaa
     1561 caacgacgtc aagtgcaccc acggctccgc gacgggccgc ctcgacgcgg acgctctctt
     1621 ctacctccgc tccaggggct tctccgagag tgaggcccgg agcgtcctga cgctcgcgtt
     1681 cgcgagcgag atcgtcgacc gcgtcccgct cgacccgctc cgggcgcacc tgaccgaacg
     1741 cgtcctcggc tggctctccg gggacgcgcc gggggcacga tgatcgacat caccctgacg
     1801 ccggccacct tcgacgtgga gacggtccgg gccgacttcc cgatcctctc gcggaaggtc
     1861 cgcggccggc ctctcgtcta cctcgacagc gcgaacacga cgcagaagcc cgaggcggtc
     1921 atccgcgcgt cggaccgctt ctaccgcgag acgaacgcga acatccaccg ctccacctac
     1981 cgcctctcgg aggaggccac ggccgcttac gaaggggcgc gggagaaggt ccgccggttc
     2041 ctgaacgccg cctcgacgcg cgaggtcgtc ttcacccgcg ggacgacgga ggcgatcaac
     2101 ctcctcgcct cgagcttcgg gcaggccttc gtgaaggccg gcgacgaggt cctgatcacc
     2161 gggatggagc accactcgaa catcgtcccg tggcagctcc tctgcgagcg gacgggagcg
     2221 cgcctgcgcg tcctcccgat cgacgaccgc ggcgagctcg tcctcgagga gctcgacgcc
     2281 ctgctcaccg agcggacgcg gatcctcggc gtcgtccacg tctccaacgc cctcggcacc
     2341 gtcaacccgg tgaaggagat cgtcgcgcgg gcgcacgcga agggagtccc cgtcctcgtc
     2401 gacgccgcgc agtcgctccc ccacctcggc gtcgacgtcg cggacctcgg ctgcgacttc
     2461 ctcgccttct ccgggcacaa ggtctacggc ccgacgggga tcggcgtcct ctgggggaag
     2521 gaggagtggc tcgagaagct ccctccctac cagggaggcg gcgacatgat cgcctccgtc
     2581 tcgttcgaga agaccaccta cgcggagctc cccgcccggc tcgaagccgg caccgggaac
     2641 ctggcgggag gcgtcgccct cggcacggcg ctcgactacg tcaccacgat cgggctcgag
     2701 aggatcgccg cgcacgagcg cgcgctcctc gcgcacgcca ccgagcgcct ctcagaggtg
     2761 ccgggcctgc ggatcgtcgg gaccgcccgc gagaaggccg gggtcgtctc gttcgtcctc
     2821 gacgacgtcc acccgcacga cgtcggcacg atcctcgacc aggaggggat ctgcatccgg
     2881 acgggccacc actgcgcgca gccggtcatg gcccgctacg acctcccggc cacggcgcgc
     2941 gcgtcgttcg gcctctacaa cacgctcgag gaggtcgacg cgctcgtgga cggcctccgg
     3001 agggtgcgcg aggtattcgg cgcatgacgg ccctgaccga cctctaccag gaggtcatcc
     3061 tcgaccacaa ccggtcgccc cggaactttc gcgcgatcga aggggcgacc cattccggcc
     3121 tggggcacaa cccgctctgc ggcgaccagc tgacgctcta cgtgagggtg gaagacggga
     3181 cgatccgcga gatcggcttc caggggaagg ggtgcgcgat ctcgaaggcc tcggcctcgc
     3241 tgatgaccga ggccgtgaag gggaagacgc tggcggaagc cgaggagctt ttcggccgct
     3301 tccacgacct cctcaccggc ccgtccgacg tcccggccga ggcgaagagc ttcggcaagc
     3361 tcgccgtctt ctcgggcgtc cgcgagttcc ccgtccgcgt gaagtgcgcg acgctcgcct
     3421 ggcacacgct ccactcggcc ctcgccggcg gctcgcccgc ctccacggag tgacgcgatg
     3481 tccgccgcaa cccccgagcc gacggccgcc acaccggccc ccgacctcga gcagagcgtc
     3541 atcggcgcgc tgaagaaggt ctacgacccg gagatcccgg tcaacatcta cgacctcggc
     3601 ctcgtctact ccgtcgtcgt cggggacggc ggcgcggtga gcgtcacgat gacgctcacc
     3661 gcgcccgcct gcccggtcgc ggggacgctc cccggcgagg tggagcgcgt cgtccgctcc
     3721 gtcgagggcg tctccgaggc gaaggtcgac ctcgtctggg acccgccgtg gaacccgggg
     3781 atgatgtcga agatggcccg ggtgatgctg gggatgtgac cgcggcccgg gcctccggcc
     3841 cgggctagcc ccgtcgcgcc aggcggcgga tcgcgaggcc gccgcagagc accgtcacgc
     3901 cggtgaggac ggccgcggcc agctcgggcg ggatgtaggt gaactgtcgg acgccggcga
     3961 ggccgagggc gagctggtcg aggtggaacg tcggccagac gatcacccaa cgcttcagcg
     4021 agtcgggtag cgggatgaag aggcccgaga gccacatcat cggcaggaag acgaggttcc
     4081 cccaggccgg agccgccgag gccgatccga acgtccccag gagcaggccg atggcgctga
     4141 acggaatcgt gccggcgaag agcacggccc agacgatcag gagctgcccc ggtgagaggg
     4201 tgatcttcgc ggccagcagc gcggcgacga cgacgagcgt gagggcgatg ccggcgacca
     4261 ccatcgccat cgcgaccttg gcgacgatcg tcgccccggg cgggagcggc agagctcgct
     4321 tgagcttcag caggttcccc tcgcgctcgg tcgccaccgt cacgccggtg aagaccccgg
     4381 gcatcgccac ggcgaggacc gcgaagccga cgaacaggta gttgtggatt cccggaccga
     4441 gctcgccgcc gcccgcgctg ccggagatca cgacgccgaa cagcaggtag atcgccaccg
     4501 ggatcgcgat gaacggcagc gcgagacccg tcgtgcgcaa cgcggcgagc acctcgaacc
     4561 tcgcctcctg gagataggcg cggaggaccc gggcgagcgg gaggggcgcg cgggccgcgg
     4621 cgagcgtcgc gccgctcacg cggcctcccg ggtgatctcg gtgaacgcct cggcgaggct
     4681 cgcctgctgg acctcgagct ggcgcaggtc ggggtcttcc gcgagcagcc ggcgcacgac
     4741 cgtctcggcg tcggacgccg tgacgttcac gaggtcggcg tcgcgggtcg cctcgacgac
     4801 gcccggccag ctcctcatcg ctccgacgtc gacgctcgag gcgcagcgga tcctcttgcg
     4861 cgtcaccagc gcccgcatct cgtcgacgct cccctccgcg acgatccgcc cccgagccag
     4921 cacgacgaca cggtcggcga gcgcctcggc ctcctcgagg tagtgggtcg tgagcacgac
     4981 cgagcagccg tcgttgcgca gcttcgcgat cgccttccac atggcctcac gcgccgggac
     5041 gtccagccca accgtcggct cgtcgaggaa caggagccgc ggccggccgc agaccgcggt
     5101 cgcgaactgg acctgccgct tctggcccgc cgagagcctg ccgtagcgcc gcctcgcgag
     5161 ctcccgcgtg ccggtgaggt cgagcgcctc ctcgacggag aggggatcgc ggtagcagct
     5221 cgacgtgaga gcgacgtgct cactcgccgt gagcgtcgga gcgagcgcca cctcctgcat
     5281 catcacgccg acgccgaggc ggctcgcggc ctcgagcggc gggccgccca tcacgagcgc
     5341 cgtaccttcg tccggctgca gcgtgccgag ccagaggccg atcgccgtcg acttgccggc
     5401 gccgttcgga ccgagcaccg cgaggagctc gccggggcgg acctcgaggt ggaggccgtc
     5461 gagcgccacc gtcgttccgt acctcttcgt cacgttcacg agcgcggcga ccgtctccgg
     5521 atcggacgtt tcgtcgacgg ggccgcccgc cgcgcccgcg agagcccgaa tgcgacgggg
     5581 gccggcgtcg cgcccggcgt cctgcaacgc tcgccgcgga tagcgccgtc cgttcaggag
     5641 ctcgccgaga gctgtcgggc gcacgaggaa gtggtagctc gaaaggagca gcgccatcgt
     5701 cacgacgagg atcgccggga gcttgatcga ccagtggagc ggcacctcca tcagcatcac
     5761 ctgaaggccg aagacgatcg gcggatgcac caggtagagc cagtacgacg cgtcggagag
     5821 gtaccggcgc acggcgctct ccccggacag gaagcggacc gccgccccca ggagcccgaa
     5881 cgtccacgtc cagatcgaga gtgcgtacgc ggccgcatag gcccatcgca tcggcgcccc
     5941 gccctcgatc ccgccggtcg tcgccgccgc ggaaaggtcc ggccggaggc cggcgatggc
     6001 gagacagacc gaggtgagag ccgccgccag gacgaggttc accggccact gccgcgcgag
     6061 cgtgtcgagc cggtcgacgc gacggtgcag cagccagccg agcccgaacg ccgtaccgta
     6121 ggcgaccatc gccgggagct gcggcatgag gccgatgtcg ggcgtcggga tcccgaacca
     6181 ggccgcccag tccgtcttcc gggagaggac cgcgaccgtc ggcgccgcga gcaatgccgg
     6241 ggcgagatag ctcgcgagaa cgcggtcgag ccaccgatcc aggcctgccc gggcgctccc
     6301 gccggcatcc agggccgcga ccccggcgcg cagcgcgagc gccaggaggt agaagatcgc
     6361 gaggtcgtag aggaaccaga gatggacgag cgggacgccc cgaggcgcgg cggccccacc
     6421 cgggtcgccg ccgggaaacg tcctcgtcag cccccaggcc acggccgcga tcgtcagcgg
     6481 cgcgaggatc acccagccgg cggtgagcgg aaccaggact cgccgggcac ggtccttgac
     6541 gaagccgcga acgccgcggc gctggaggag cagtcggccg aagaagccgg cgatgacgaa
     6601 gaagagcgtc atccggaacg tatggatgac gaagaacgcc acggcgggca ccgtgccctg
     6661 agagacgtcc cgggcgggga cgacgaagaa gaacgacatc gttgcgtgga ggacgatgcc
     6721 gaggaggagc gccccggcgc gaacggcgtc caatgcgtgc aggcggtcgg gattcgtcgg
     6781 gcgattcatg gccggctcct cagagcgcgt ctctgcgacg cgacggaacg tagaaggcga
     6841 gggccagaga cagcaggatc acgacgagct cgaccgccag cacggcgagg acctcggggc
     6901 tccaggtcgc gacggcgtcc ttcgagcgcc cggcgacgcc ggggagccgg gcgagccggg
     6961 acatgaagac cgtcaccgag aagttcgtga cgaggatgcc ggcgatcgcc gccggctccg
     7021 accgggtgat gacggtgagg gccatgagca ggcagaagtt gacgagcagg aacgtcatcg
     7081 tcgcgacgaa gaacgggagg gagccgtccg gagccgggga gagcgcggtc acggcggcgg
     7141 cgccgccggt gagcgccgtc cacggcagga ggaaggcggc gagcgcggac gcgaccttcg
     7201 cgaacgagta ctgcgccgcc gacaccggca ggctgaggac gaagaggtcc gacctcgtct
     7261 cgtgctcctt gaggacgagc atcggcaggg cgacgccgag aacgatgacc gtcgtgagga
     7321 gcagcaggaa gccgacgttc ggcccggtcg agatgccgtc gccgtcgccg aagcgcgtca
     7381 ggacgagcga cgccgcgccg gcgatcgcgg ttccgagtat cagccagcgg tagtggtgga
     7441 ggtccctggc gatcagccgg gcggtgagag atcctcgcga gccggtcatt cgccgcgctc
     7501 ctttcgcctg ttcatgacgg tggcgacgaa gatctcctcg agtgtcatcc gctggacgtc
     7561 ccgtacgacc gcgccggccc gctggtacgc cgccggcagc gcctcttcga aggcgttgac
     7621 cgtgacgacc gccgtgcgcg cgctccgcgc gacgtcgacg actcccggca cctccgggag
     7681 cgcgatcgtc gccggaagct cgaggctcag gcgcctccaa cgctcgagga acgcctcctt
     7741 gtcgcccgag tcgacgatcc gcccgcggtc gatgaaggcg atctggtcgc agatctgctc
     7801 gacgtcgagg gtgttgtgtg acgagaagag gatcgagcgt tcttcgtccc ggacgacctc
     7861 catgagctcg ccgatgacct cctggcgggc gacggggtcg aggccgacgg tcggttcgtc
     7921 gagcacgaga aggcgcggcc tgcgcgccag cgcgaggagc agggccgcct tgacctgctc
     7981 gccgtgcgac agccccttga cccgctgctc gcggtggagg tggaaccgct ggaggaggcg
     8041 ggcggcatag cggtcgtccc aggcgccggg gaagacggaa cgcacgaagc cgacgtgcca
     8101 gccgatcgtc gcccccggga acagccgcat ctcctcggag acgaagccga cctcccgctt
     8161 cgcgcggacc tgctcgtcgg gcatgcggca gccgaggacc tcgacgtcgc cggcatccgg
     8221 acggacgagg gccatgagaa tccggatcgt ggtgctcttg ccggcgccgt tcgggccgac
     8281 gaagcccagg atctgccccg gctcgaggcg gaaggagacg tcgtcgagcg cgaagaagcg
     8341 atagcgcttc gagacgccgc ggagggcgat cgccggagtc gcggtcgtgg tcatcggtct
     8401 tcttccctct ggcggacgac tttgcgaagc cgctgctcga gctccctcgt cccgaggccg
     8461 agctcacgtc ccacgtcgac ggctctcgcg aggtggccgt cgagctcctg ttcccggagg
     8521 tcctgctgga gcttcacgtt ctcggcgacg aacgtccccc ggccctgccg ggtggtgagg
     8581 accccctggt gctcgagctc gaggtaggcg cgcttgacgg tgatcacgct cacgcgcgtg
     8641 gacgcggcga gcgcgcggat cgacgggatc tcctgccccg gctcccagtc gccggccgcg
     8701 atgcgccggc agacctgctc gatgatctgc tggtacatcg gcctcgggtc ggcctgcgag
     8761 atggtgagct cagcgtgcat atcaatatgc acactataca cggctttcga gaacgggcaa
     8821 gcgcccggcc catctcccgg gtcggggagc acgccgggga gggccgccgg ccgtcgggca
     8881 cgatcccgcg ccgataatcg acctctcctt cgaatgcgac gcgagacgcg accgtgaacc
     8941 tgatcctcgt cctccccgat gagctcgaag cggacggcac cgtgaggctg accggccgcc
     9001 gtgcccgcca cgtccacgac gtccaccgcg cccgcgtcgg cgacgagctc gtcgtcggcc
     9061 tgcggaacgg cgccaccggg agagggacgg tgctcgccgt gtcggccgac gaggtgcggc
     9121 tcgcggtcga gctcgtcggg gagccaccgc ccccctccgg cgtcgacctc ctcctcgcga
     9181 tgccgcggcc caaggtgctc cggcgcgccc tccaggcggc cgcgtcgctc ggcgcgaagc
     9241 ggatcgtcct cgtgaacgcc gcgcgtgtcg aaaagagcta cttcgacacc ccgtttctcc
     9301 agccggagga gatcgagcgg aacctcgtcc tcggcctcga gcaggcgcgc gacacgctcc
     9361 tcccggagct cctcgtccgg ccgcgcttcc gaccgttcgt ggaggacgag ctcgacacgc
     9421 tctggccgag cgactccttc ggacggctcc tcgcgcatcc cgccgacgct ccggcgctcg
     9481 gcgcgccgga cgcgccccgc gtcggcccgc gctccgtcgt cgcgatcggc cccgagggcg
     9541 gctgggtccc gttcgagacc gacctcctcg aagagcgcgg cttcgcacgg ttcacgctcg
     9601 gcccgcgtgt cctccgcgtc gagacggcgg tcgcgttcgc gttcggacag ctgcttctct
     9661 cgcgcgcgct ccaaagcggc cgctgacgcg ccgggtgtgc gatcccggta cgatcgcgga
     9721 accatgcgac tcgagtacgg cctcgcgctc ggactcctcc tcgccgttcc cgccttcgcc
     9781 caggacggcc cgccgcccgt cccggctgcc ccgatctccg cgccgtcgcc cggcgcgccg
     9841 taccgcgaca cggtcctctc gggccgcacc acgaacgcgg ccggcctctc gttcacggtc
     9901 ggcgccgcgc gctacgaggt cgcgacgggg agtctctggg aggtgacgac gaaggacgga
     9961 acgcccgtcg gcgcgttctt ccgcggagcg gggacgctcg cgttctcggc gggagacccg
    10021 caggccgcgc gtctcgccgc ccggaacgcg aagcacgtcg gcggcgcgaa ggaggtcgac
    10081 ggggagctcc gggcggcctt ctcgagagcc acgttcctct tctccccggc gcttcgcccc
    10141 gcgtggtcgt acgccgccgg ggaggagccc ccggtccgcg tcttcgcgac gcacctcgag
    10201 cggttcgagc acgaccggac gccgcaggtc gcctcccgcc tcgcgatcgc cgaggcggcg
    10261 cagggggcgt tcttcgcggc gacgctcgag gccgacccgg acctccgcca cgtctacgac
    10321 cccgtgaccg acgacgagga ggtgctccgc gtcgtcgacc ggccggccgg cctcccgacg
    10381 ggcttcccgg agatgcgttt cgcccgggac ctctcccgtc ggcccctcgg acggacgcgc
    10441 cggcaggcgc cgcgcgtcga cgtccggctc gtcgccgtgg acgtcgacgt gcgcgaaggg
    10501 acggccccgt ggggcgagct gaaagtgacg gagaccttcg tcgccgtccg ccccgtctcg
    10561 ttcctcgtcc tcgacttcgc gaccgagacg atctatcgga acgacctcct cgagacgcgc
    10621 cttcgcgccc tcaccgacgg agagggccgg ccgcttccct gggcgctcgg agacggcgag
    10681 ctcgtcgtgg ccctcccgaa ggccctcgct cccggcgcga ggttcacgct ccgcctcgac
    10741 tacgaggcgc cctatttcga gcgggccggc ggagacaacc tctgggagct cccgatcgcg
    10801 agcgggtggt acccgcagcc gcccttcttc aacgcgtcgc accacacgtt ccacgccgtc
    10861 gtccgcgccc gcaagccgct cctcccgttc gcctccgggg acacggtccg gcggtccgag
    10921 gaggacggtt ggaacgtcct cgagacgcgg ctcgagaggc ccgtcccgtt cgcgaccgtc
    10981 ctcgccggca aatacacgac gcaggagtcg accgaggacg gcgtcacctg ccgcgtcgcg
    11041 agctacggca tcccgaagga gatgtcgggg aagatgctcc tgagcgtctt ccacggggtc
    11101 cgcaagttct acgagtggct cctcgggccg tttccgtgga aggagttcac gatcgtcgag
    11161 atcaacgcct acggcttcgg ccaggcgcct cccggaatga tgcggatcac gaaggaggcg
    11221 tttcagtcga gcatcttcac ggacgagatc tcgtccctgt tctcgcacgg gatcaaccag
    11281 cgcgtcgcgc acgagatcgc ccacgcctac ttcgggtacg tcgtcgccga cgcctccccc
    11341 cagcaccagt ggatctcgga ggcgttctcc gagatcgcct cgatgtacgc gatcgagaag
    11401 ctgaagggga aggcggaggg gaagaagctc gccgggacgt gggccggggc cgcgcggcat
    11461 tcggcgaaag cggccccgat cgacctcgcg aacgacctgg cgccgaagat cgcctcgacc
    11521 tgggacagct cgaccgcgat cgaccgggcc gagctcgtct actcgaaggg cgcgcacctc
    11581 ctccagacgc tccggctcga gctgggtgac gacctcttct tcaccgtcct ccgctcgttc
    11641 ctcaggagct tcgagaagca gcgggacgtg acgaccgacg acttcgtcgc cctcctctcg
    11701 ttcgccacga agaaggactg gaagccctgg ttcgagaagt actactacgg caccgagatg
    11761 ccctgaggga ccggccccgc ctcagtcctt cttccccttc acccacgtct cgagccacgt
    11821 gtccgtctcc cagagcatgt ggaggatgtt ctcccgcgcc gcgtagccgt ggctctcgaa
    11881 cgggaggagg acgaggcggg agcgaccgcc gagcccctgg atcgcctcga agagccgctc
    11941 gctctggagc gtgaacgtcc ccgggttgtt gtccgcgtcg ccgtggatca ggaggatcgg
    12001 ctcgtccagc gtcgccgcgt ggtcgaacgg cgacatgcgc gagtagacgt cccgggcctg
    12061 ccagtaggtc cgctcctcgg cctggaagcc gaacggcgtg agggagcggt tgtaggcccc
    12121 ggagcgggcg atcccggcgg cgaagaggtc cgagtgcgcg aggaggttcg cggtcatgaa
    12181 cgcgccgtag gagtgccccg tgacggcgca gcgcttcggg tcgacgatcc cttccttctc
    12241 gagcgcgtcg atcgccgcct tcgcgttcgc cacgagctgc tcgacgaacg tgtcgttcgg
    12301 ctccttctcc ccctcgccga cgatcgggaa ctgtgcgttc tcgaggacgg cgtagccgcg
    12361 gagcgcccag aagacgggcg agccccagta gggccggacg aacgcgtgcg gggaggagtc
    12421 gaccatgccg gccgcggcct tgtccttgta ctccgtcggg taggcctcga ggaggagcgg
    12481 aaggcgcccg tcgcgcttcg ggtcgtagcc gggcggcagg tggaggtcgc ccgcgaggtc
    12541 gatgccgtcg gccctcctgt agcggacctt cctcgtcgcc accgcctcga ggggcttgta
    12601 agggttctcg aacgacgtga cggggcgcgg cgcgatccgc gccttcacgt tccggacctg
    12661 gtagttcggg aagagcttcg acccctcgac ccgcgtcagg aggacgcccc gctcggcgtc
    12721 gaggacccgg acgatccgct cgtacgtcga cgttccgtcc gcgcgccaga ggcgcgtcgt
    12781 cttcccggtc gcgacgtcgt acgcgtcgag gaacggccgg ttcccctcgg gcgagtagcc
    12841 ctcgccggag aggaagagcc gcttcccgtc gcggctcgta aggaggagag gccggccccg
    12901 gccgtccact cgcgtgacga agtcgcccgg cagggcgtag aggttctcgg aggagaggtc
    12961 gaagaggacc ttcggctccg agtcgccggc gtcggccatc gcgaggtagg tcttcgtgtt
    13021 gcggtccttc cagcggaagt cggagaggac ggcgacgcct cccgcgcccc aggtgacctc
    13081 gctgaaccgg ttgcgcgtct tcgcaaacgg gcggggcgcc ccgtcgaacg gagcgtcgag
    13141 gaggaacagg cggtcgcgga acgggacgtc gttcgcgggg tcgccgccgt cctgagcctc
    13201 ggcgaagacg atcgtcgccg gcgcgtcgtc gcgccagtcg aactcgcggc gccccgcctc
    13261 cgtcgcgtcg aacgcgaccg ggatcgcgtc ctgcaccggc ttctcgacga gcgtcgccgc
    13321 gagcttcccc gccgcgtcga ggacgtcgac gcggtagccg aagcggtaga ccgggaagcg
    13381 ataggagaac ggcgggacga tcgtcgtcgc gaggatccac ttcccgtcgg gggaaggccg
    13441 caccgatcgg cggatcgcgg gcgggaggat cgcggcgctc gtcccgtcga gcgcgaagcg
    13501 gcggacctcg gtcgtcgcgt agtgcgcgaa cgtcttctcg tcgttctcgc tcttcagcag
    13561 gtcctgccag gtccgggccg gcgccttgcg ccccgcggcg accttcacga ccgggccggc
    13621 ggggagctcc gccgacgggg tgaacggctc gagggacgga cggacgtgga ccaggagccc
    13681 ggactcgtcg gggagccact ggtacgggaa gtcgagaacg gccgagacgc tcgccggcgt
    13741 gacgcgcgaa gccctcgcgc tcgcgaggtc gacgacccag agggagaggc cgtcggcatc
    13801 gcactggacg aacgaaaagt acgtcccctt cggcgagaag cgggtccact ggatgcgcgg
    13861 cgcggccggg agcccctcga cgcgcgcgct ccttccggaa gcgatctcct tcacggagat
    13921 ccccgtcgtg acgttgaggc gggcgcggtt gtggctcttc gggttgatcc gcagcccggc
    13981 gagccggagc tcgggctccg cgacttccgc gagcgtcttg tagccgggga ggtcgaggag
    14041 gacgagccgc gctccggccg ggtcgacgag gacggagggc ggcagcggcg cgtcgacgag
    14101 cttccggatc tcctcgggcg gagtgcggaa ggcgacgttc tcctgggcgg cggccgtcgc
    14161 ggcgacggcg aggaacggga agagggcgag ggtcgggacg aggcttcggc gcaccatccc
    14221 gcaaggctac cccagccccg ccccggccac ggcgggcgcc cgcccgttca ggcgggccag
    14281 acgaacgtca tcggcacctc gacgttcggg ccgaggctct gcgtcacagg gcaggcgcgg
    14341 gccgtggcct cgaggagggc cctcttctcg gcggggagcg acgcgaggga cggcgggaac
    14401 gtcaccgtcg tcgggagcga cgcgatccgc cggcgcgggt cggcgaccat ctccttgacg
    14461 acgtggacgg tcgtcccggc gagctcgatg ccgtggctcg tcgcgacgag ggccatcgtc
    14521 gtgacgaggc aggagccgag cgaggtcgcg cagaggtcgg tcggcgagaa cgcctcgccc
    14581 tttccgccgt tgtcgagcgg ggcgtcggtg acgagcttcg agccggaggg gcgatgggtc
    14641 gcctcgcagt ggaggccccc ggtgtagagg atgtcaatgt cgacggccat gccggagacg
    14701 ctacgcgacg ccggccgtcc cgtcgagccc cgccgcgcgc gccgcgggac ggctcagccc
    14761 gggccgagcg gaggcgcccc gccgctcccc ggctcctcgt gcagccggaa ccagcgaagg
    14821 ccgcgcggga cgatcccgag gaggttgacg gcgagcgcga gcccggcgag gacgaggaag
    14881 tcgccgaggt tccggccgag gacgaagagg acgacaccca gtatcgcgga aacctccgcg
    14941 accgcgagcg agacgagcgt cttccggagg aactcggggt acgccgggct cgtcgcgacg
    15001 tcgacgccga cgcgcggcgc gaggacgagc gcgaggagcg ccgctccgaa cgcggcgccg
    15061 tagaccgcgt agcgaagccc tatcggcgcg gatgcgcccg gggcgcccgt cgtcgcgagg
    15121 acgaagacga cgacggcgta cagcgggatc gacgcggtca ggccggcgac gaggacgagc
    15181 cagcgtgctt tctcggggag gggtctcggt cggaccatgc tctcgctccg gcccgacgat
    15241 acccccactc acgcccggcg cggcgtctcg ggcccgaggg accgaaccga ctccgtgatc
    15301 cgcccgaccg cgcgacccgg cttgccgcgg cagccatgtc cgaaaaccgc cagacacggc
    15361 cggccccctg cgcgacactt cgcccgtgga gatcgaccgg gagtcctgcc tcgccgcctt
    15421 cctcggacgc gaccgccggt tcgcggggcg cttctacgcc ggagtgacgt cgaccggcgt
    15481 ctactgccgg cccggctgcc ccgccccgct cccgaggccc tcgaacgtcc ggttctatcc
    15541 gtgcgcggcc gccgccgagg aggccgggtt ccgggcctgc cgccgctgcc ggcccgagac
    15601 ggctcccggg acgccggcct gggcggggac gtcgacgacc gtcgcccgcg cgctccgcca
    15661 gatcgagtcg ggcctcgtcg accacgcgcc gctcggcgtc cttgcggccc ggctcggcgt
    15721 cggcgagcgg cacctccggc gtctgttcgc ggaacatctc ggggcctccc ctgtcgccgt
    15781 cgcgaagacg cgccggcttc acttcgcgag gcgccttctc gacgagacgc cgctccggat
    15841 gtcggacgtc gcgctcgccg ccgggttctc gagcgtgcgc cggttcaacg aggcgatgcg
    15901 cgcggcgttc ggggccccgc cgaccgtgct gaggcgcgcc tttgccggct ctcccgcatc
    15961 cgcggccggc gtgacgctgc gcctcccgta tcgcgctccg ttcgactccg tcgagctgtt
    16021 cgcgttcctc ggcgcccgcg cgattcccgg cgtcgagtcg gtcgagggca gcctctggcg
    16081 ccggacggcg gagctcgcgg gagtcgccgg gaccgtcgag gcggaggtcg acgcggcgcg
    16141 ctcctcgatc gtggtgcgcc tcttcgggct cccgcccggc gcggcgctcg acgcggcgcg
    16201 ccgggccgga cgcctcttcg acaccgatgc cgacccggcc gcgatcgcgg cgcacctcgg
    16261 gagggacccg ctccttgcgc cgctcgtcga gcttcggccg ggactccgcg ttcccggcgc
    16321 gtgggacccg ttcgagacgg cggtgaggac ggtcctcggc cagcaggtgt cggtgaaggc
    16381 cgcgcggacg ctcgccgcgc gcctcgtctc gaggttcggc cggccgctcc ctccgggcgt
    16441 cgcgcgcggc tccctcacgc acctcttccc gcgcccggac gacctcgccg aggccgacgt
    16501 cgcgtcgatc gggctcccgt cgcgccgggg cgagacgatc cgcgtcctcg cgcgggaggt
    16561 cgccgccggc cgcctcgacc tcggcccggc cgcgtcgctc gaggaggcgg aacggcgcct
    16621 cgcggcgatc cccggcgtcg gaccctggac ggcgcaggcg atcgcgctcc gctcgctcgg
    16681 cgagcccgac gcgttcccgg ggggcgacct cggggtccgc aaggcgctcg cggtgcgggg
    16741 cgtgcccccg acgaccgccg cggccgaggc gcgggccgcg gcctggcgtc cctggcgggc
    16801 ctacgcgctc gtccacctct ggaccgacct cgcatcacgg gaggactcat gacgaccacg
    16861 ctcgagaccg ccacgatcga cacgcccgcg ggagggttct ggctcgccgc ccgcgacggc
    16921 gcgctcgtcg ccgccgagtt cgccgaacgc gtgccgcacc gcctctcgcg gctcgaggcc
    16981 cgcttcggcc cgctcgagct ccgggacgcg gccgaccccg ccggcgccgc gagcgcgttg
    17041 cggcgattcc tctccggcga tctgaacgcg ctcgaggcgg tccccctcga cgccggcggc
    17101 acgccgttcc agcgcgacgt ctgggccgcg ctccgcgaga tcccggctgg cacgacggcg
    17161 acgtacgccg acctcgccgc gcgcgtcgga aggccggcgg ctgtgagggc cgtcggaggg
    17221 gcgaacggcg cgaacccggt cgccgtcttc gtcccgtgcc accgcgtcgt cggaaaggac
    17281 ggcctccgcg gctacgccgg aggtctcgag cgcaaggagt ggctcctcgc gcacgaggcg
    17341 acggcgaccc ggacctgagg agacgaggcg tcccgcgccg cgaggccctc cccccgcgcg
    17401 ccggcccgtc tcagcggggc gcgccggggg ctcggccgat ccgccggacc tgggcctcgt
    17461 ggtgccgggc gtgctcgtgg aggaagatga gcgtcccggc cgggtcgaga cccccggcgg
    17521 cggggtgccg gaagaagagc gccgcgcggc gctcctcggg caggccgagg aggagggtcc
    17581 ggaggtcgcg tcgggactgg tcccatcgcg tggcgctcgt ctcgagcggc agggtctccg
    17641 acgggacgac ctgcgccacg ggggcccgga ctcggatcgg cgagcggagg accccgacga
    17701 ggagcgcgag cttcccccgg tcgacgatcg agatcggctg cggcggcgcg aggagcttct
    17761 tcctcgcgta ggcgaggacg ccctcctcga cgaggacgag gtgctggacg acgtcgagcg
    17821 cgttccagcc gacgccgtcc gcccggaacg cgaggcgctc ggggctcgtg gccgagagtg
    17881 cgccgaggaa cgcgtccttc catcggtcca ggtcgtcgag gcgcttctcg atccggttct
    17941 tcacggcgcg gagtcttcca cgaagacgcc gcgccgtgaa gcgcgccggg aggcgctcag
    18001 tgcgtcggcg gcgcgccggt cccggttccg gccggctcct cggcgaactt cagctggccg
    18061 cccgagaccc gtccgcgaag cgagacggcg agcgcctcgc tcaggtcctg caccgggacg
    18121 atctcgagcc cgtcgcgcac gtcctgcggg aggtcctcga ggtcggggac gttctccttc
    18181 gggaggacga ccgtccggat cccggcccgg accgcgccga ggagcttctc cttcacgccg
    18241 ccgatcggga ggacccgccc ggagagcgtc acctccccgg tcatcgcgac gtcccgccgg
    18301 cagggccgct tcgagagcgc cgagacgagg gccgaggcca tcgcgacgcc ggccgacgga
    18361 ccgtccttcg ggatcgctcc cgccgggacg tggatgtgca ggtcacgctc gaacgaagtg
    18421 tcgtcgatcc cgaggagcgc ggcgtgcgag cgggcgaacg tccaggcggc ccgcgccgac
    18481 tccttcatga cgtcgccgag ctggccggtc aggaggacct cccccttgcc gcgcatcagc
    18541 gccgcctcga tgaacatgat gtcgccgccg gccggcgtgt agtacatccc cgtggcgacg
    18601 cccacctggt cttcgtggac gatcttctcg ggctggatct tcttgcggcc gaggatcggc
    18661 gccacgtcgg ccgcgtcgac ggccgccttc tcgatctcgc ccgcggcgat ccggcgcgcc
    18721 accttccgcc cgagcttccc gagctcgcgc tcgagctggc ggacgcccgc ctcgcgcgtg
    18781 taggacgaga tgatctccgc gagcgccgcg tcggtcacct cgagctggcc ggcgccgagc
    18841 ccgttctccg agagctgccg cggcagcagg tagttccggg cgatgagggt cttctcccgc
    18901 tccgtgtagc ccgagaactc gatgacctcg agccggtcga ggagcggccc cgggatcgac
    18961 tggacgaagt tcgccgtcgc gacgaagagg acttccgaga ggtcgaacgg gacgttcagg
    19021 tagtggtcgg tgaagctgtc gttctgcgcc gggtcgagga cctcgaggag cgcggaggcc
    19081 gggtccccct ggaacgacac gccgagcttg tcgacctcgt cgaggaggaa gaccgggttc
    19141 ttcgtcccgg cctggcgcat cccctggagg atccgcccgg gcatcgcgcc gacgtaggtt
    19201 cgcctgtggc cgcggatgtc cgcttcgtca cgggcgccgc cgagggagat ccggacgtac
    19261 ttgcgtccga gggcgcgggc gatcgacttc gcgatcgagg tctttccgac cccgggaggg
    19321 cccgcgaaga ggaggatgcg gccgcggttc ggctgcttct caccggccgg tgtcggggcg
    19381 ggcaccccgg cgccggcggg agcgtccgcg ggagcgccgt ccgcggacgg cgcggcggcg
    19441 gcttccgtct tccggccgag gagctggcgg accgcgagga actcgaggat ccggtccttc
    19501 acgtccttca gggcgtagtg gtcctcgtcg aggatccgct gcgcctcgac gacgtcgagg
    19561 tgctcctcgc tccgcgccga ccacggcagc tcggccacga gctcgaggta gttccgcacg
    19621 acctgggctt ccatcgactc gcgcgagagg cgctggagct tcttccactc ccggtcgatc
    19681 tccttgcgga cctcctcggt caggacgagc gcgtcgagct tctccttcag gcccttcagg
    19741 tcgtcgccgt cgtcctcgcc ttctcccagc tccttctgga tcgccttcag ctgctggcgg
    19801 aggaacatct cgcgctggcg gtcgccgagc tcctcctgga ccttcgactt gatctcctcc
    19861 tgcgcgtcga ggacgtcgat ctgccgctgg acgtggacga gcgtccgccg gacgcggtct
    19921 tcgacgggca gcgtctcgag caggagctgc ctctccgcgg tcgggatgtc gatgtagccg
    19981 gccacgaggt cggcgagccg tccggggtcg tccgcttcgg tcaggacgtt cccgacggcc
    20041 tcgtcgggca agccgagctt cttcgcgagc tccgcggaac gctcccggag ctccttcagg
    20101 agcgcgacga acgccgtgtc ggcggagtcg agaggcggca tctcctccgc ctcaacgacg
    20161 accgcctcga gccgcccttc cttctcctcg atccgcatcg cgatgccgcg cgccttcccc
    20221 tggaggagga gccgggcgcc gccgacgccg cgctggatcg gcccgatcga cgcgatcgtc
    20281 ccgacggtgt ggagcagctc gggcgtcacc gcctcgaggt cctgcctctg cgtgacggcg
    20341 aagacgagct gctccgggct cttcatcgcg gtctcgatcg catggagagt cccggggcgt
    20401 ccggcggcga tcggaaagct gactccggga aagagaaccg cgttgcggag cggaatgacg
    20461 gggagaacct tcgatggatg cattcggacc tttcgagcca acctaacgtg ctggactcaa
    20521 gaataaactt tagtaaatct ggcttagatt ttcaagagtc tcgtctaagc gccacatctc
    20581 attgacgctg agaacgttga cgcggattcc ccgcgcggcc atcgggcatc gccgacgagc
    20641 cggaccgcgc tcctcctcgc cctcgatgcc ctcgtcgaca cgccgctcta cggcgagcgc
    20701 acgccctcga gggcgccttc cgccgcctcg cggaaagctc gggagacccc gccgactccg
    20761 agcttcacgc ccccgaagat tcgtgacacg agcaggccgc cctcgaggtc gtcgcgcttc
    20821 agaagctcca ggaggaggcg tccggggtgg ccgacttccc cgtcgtcctt cgacttctcg
    20881 acgagagctc ccgccgcgtc gcggccgcgg aaggcccagc agtggtggtt cgccttgcgg
    20941 tgctcgcccc gtgcggcctt cacggcgtcg aggacgtccg cctccgcggc cgccgggaat
    21001 gcgacggcga agaagcggga ccgttcgacc gtggttcgaa acgaggaggt tcgctcgatc
    21061 ctcacgcgcc gacggtattc gagtcggacc cgagcgtcca atcaccgaat ccgccaaacg
    21121 agagtggcct gaagtcgccg ggacgacgaa cccgaggctc cgaaccccat gagcctcgaa
    21181 ttgtcgcgag ctccctcccg agagccgctc ccggccaccg catcccgggc ctcgaatcgt
    21241 cggttgacga ataacgctag acgcgtctaa catccggcgc ccctttttgc gagtcaatct
    21301 caaaatctga aaggcacccg atgcactctc acctcccgac ccgacccggc tcccggcttt
    21361 cggtctcgct cgtcgtcttc ctcgcggtct tcgccctcgc cgcacacggc agcgcaggca
    21421 cggccaccgt taccggaaac gtcaccgatc ccttcggggc gcccgtgccc gaagcacgcg
    21481 tcacggtttc cgcggaagac ggcggcgttc gcgcggtcgc gacgacctcg ctcgacgggg
    21541 ccttccgggt cgcggagctg gcgggagggc gctacacgat cacggccgag gcggccggat
    21601 tcgccccgta cgcgaaggcc ggcgtcgcgc tcggtgcgga cagggagacg cgcctcgacg
    21661 tccgcctctc gctgacccgc ttcgagggct ccgccacggt cgaggccgag cccgggaact
    21721 tcctcatcga ggagaaggag gtctctctcc tgaagccgct cgacaaggcc ggccttctct
    21781 cccacgagtc gatggtcgcg gtcggcggcg gatcgtccgt cgcgcagaag atctacgtcc
    21841 gcggcttcga ggacacgctc ctgaacgtga cggtcgacgg cgcggcgcag gcgggcgagc
    21901 tctaccacca ccagggacgc gtccagatcg agcccgagtt cctgaaggcg atcgagctcg
    21961 aggccggcgc cggtccggcc acgagcggcg cgggcgccct cacgggggcg ctccgcctca
    22021 cgagcaagga cgcgttcgac cacctccccc ccggccggaa ggtcggcgcc ctcgtgaagg
    22081 cgaccgggga gctgaacggc gacgacgcgt tcggcggcgt cgtctcgctc tacgggaagc
    22141 tctcgcagaa cctcggcctc gtcgtctcgg gcgtcgcccg cagcgggaac gactacgcgg
    22201 acgggaacgg cgacgtcgcc gcgccgacgg cctacgacca gaagcgcggg cacgcgaagc
    22261 tgaccggcct cttcgacgcg caccggttcg acctctcgta cgagcggctg accgacgagg
    22321 ggacctacta cgaacggccg aacctgacga acttcacagg gacctacgtc ctctccgacc
    22381 acgagatgtc ccgggacacc gtctcctaca accaccgctt cgaccccgcc ggcgacgccc
    22441 tcgacctcgc gggcacggcc tactggaccc gctccgagtt ccacaacgcc aggacgacga
    22501 cgggcgcgct ctacggagcc ggagagttcg cgagcttcgg gctcgacctc cggaacacgg
    22561 cgcgcttcgg ccggcacgtc ctcgtcggcg gaatcgaggc gaggcgtgac gacgcgagcg
    22621 gcacgcagca ggccacgccg ccgcgcttct ggggaagctc ggaccaggaa gcgagcgttc
    22681 tcggcctcta cctccaggac cattggcaga tccacccgat cgtcgccctc tcggccggac
    22741 tccgctggga ctcctaccgg cacgaggtcg tcgccggcgt cggcgccggg gcgcggaacg
    22801 acgactcggg cttcagcccg aacgtctcgg tctccgtcac gccgacgacc tggctgacgc
    22861 tccgcgggtc gtacgcccgt gcgttccgcg gcatcacgat ccgcgaggcg ttcttcagcg
    22921 gcctctacgt ccaccgcggg ggcctcgacc cggaacgcgc cgacaacctc gaagccgggt
    22981 tcgacgtccg gcgcgggccc tggttcgtcc gcgggacgct cttccgccag aacatccacg
    23041 actacatcaa cgccgtctac gaaggcgtca cggcctgggg ctactgggag aacgtcggcg
    23101 acgcgaaggt ggagggctac gaggtcgaga ccggcgcccg ctgggggagc ctctccgtcg
    23161 cggccggcgt ctgggactcg aagaacacga tcgccggccg cccgatgacg gacgccgacc
    23221 tcggcctcgg gacgagcatc ggccggacct ggacgcttcg cgccgactgg tcggcccccg
    23281 ggccacggct cgacctcggg cttcgcgtcc gcctcgtcga agacgagccg aacgccatcg
    23341 ccccgaccgc gcccgacaag gacggctacg ccgtgaccga cctccacacc tcctgggagc
    23401 cgatcgacgg gctcggcctg accgtcggcg cctcggtcac gaacctcttc gacgcgtact
    23461 actacgacca ggcgacctac acgacgaacg cggcgggcgc gaagctcggg ttcgccgcga
    23521 aggggcgcgc cttcgtcctt tcgaccgcct tccgtttctg accgggcgcc gcctccgtcg
    23581 ctcatgaccg ccgctcaccg cccccgcgcc tccggcagag ctcgcgaggt cgtcttctgg
    23641 gtccacctcg cgagcggcct cgtcgccggg ctcgtcgtcg ccgtcatgtc ggcgacgggc
    23701 gtcgccatcg cgttcgagca cgaaatcctc gccttcttcg accgccgtgt cgcgcgcgtc
    23761 gcggcgccgg aaggggcgag gcgcctctcc ctcgacgcgc tcgtctcgag cgccgccgag
    23821 aagcgccccg ggttccgccc gacgctcttc ctcgtcccct ccgacccgac ctccgccctc
    23881 gagctgcgcg ccggccgcga gggggccctc tacgtcgacc cgtactccgg ctccgtctcc
    23941 gacccccgct cgggtgcggc gcacgagatc ctccacacgc tcgaggagtg gcaccggtgg
    24001 ctcggcctcg aaggccgcgg ccaggccgtc gggaagctcg tgacgggcgt ctgcaacgcc
    24061 gcgctcgtcc tcctctgcct gacggggctc tgggtctggc tccccctcct cccggccggc
    24121 cggtcggcct ggaggcgccg gctctggttc aggcgcggcc tcgaggggcc ggcccgcgac
    24181 cgaagctggc acgacgtcgt cggcttctgg acgcttcccg tcctcgcgct cctcgccggg
    24241 accgccgtcg tcttctcgtt cgaatggggt caccgcctcg tcttcgcggc cttcggcgaa
    24301 gaggcgcccg cggcgcgcgg tcccgggatg ctcgcgggcc cgcccgtctc tctgcccgac
    24361 ggccctcccg gccgggcgcc gctctcgctc gaggcgatcc acgccgccgc tgccgcccgg
    24421 cacccggggc acgaggcgat cgccctgccc ctcgcacgga ccgccggccc ggtccgcccg
    24481 ctcgacgtcg tcgtcttcga gcccgctccg ttccagacgc gcggccgggt ccagctgaag
    24541 gtcgacccct tcaccggaga cgtcctctcc acgttcggct gggccgaccg gagcgccggg
    24601 acgcgcgccc gggtcctgat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nntccacgtt cggctgggcc gaccggagcg ccgggacgcg cgcccgggtc ctgatccgtt
    24781 tcctccacac gggcgaggcg ctcgggtggc cgggacggat cgtcgccgtc gccgcgagcg
    24841 cgctctccct cgttctcgtc tacaccggat tcacgctctc ctgggcacgc ttccggaggt
    24901 ggcgcgcccg gcgcagacgc gacctcccgg cgtccgagga ggtccgggcc gcattctgag
    24961 aacgcccccc gataatcgga cagaccgatg ccgggactct tcgtcgcgca gcgcgctggc
    25021 cgactccggc tcgtcgcggc cctcctgacg gccctcgttt cggcgccgtc cttcgcgggc
    25081 gagcccgtcg cccctccctg cggaacggcg gcggagctga aggccgcgct cgcccgcgcc
    25141 gccgacgagg cggcgcgggc ccggatcgcg aacggcttcg ccgactgcgc cgcccgcgcg
    25201 acaacgcccc tcctctcgcc cggacgctcg ccggggagcg tccgcgccct cttcgtcgtt
    25261 cgctcgaacg ccgagaaggt cttcctcgcg ggcgacatga acggctggag cacctcggcc
    25321 cagcccttcg aacggctcgc gggaacagac ctccacctcc tcgaggtcga ggtccccgac
    25381 ggcgcgcgcc tcgactacaa gctcgtcgtc tcgggaacgg agtggacgct cgacccgtgg
    25441 aacccgcgga cgatgaccgg tggctacggc ccgaactccg agctccggac cccggcgtac
    25501 gtcccgcccg cccaggtcga gccggggaca ccgaccgcga acgggagctg ggcggagctg
    25561 aagatcgaga gccgggcgat gaacggcccc cggaccgcct tcgtctggtc gtccgcgccc
    25621 gcgggggcga cgggaccgta cccggtactc tacctcctcg acggcaccga ctaccgcgac
    25681 ttcgcgaagg cccacgtcgt cgccgggaac ctcgtcggcg cgggccggct cccacccctc
    25741 gtcctcgtcc tcgtccctcc cgtcgaccgg cgcgcggagt acgacgacga tcgctcggcg
    25801 ccggacggag gcggagcgaa cggaccgcgg ggccgggagg cgcccggcgt cgcccctccg
    25861 cacgcggcct tcacgcgatt cctcgtcgac gagctcgtcc ccgccgtcga agcccgctgg
    25921 cctgtccgga aggatgccgc ggggcgcggc gtgatggggg cctcgctcgg aggcttcgcc
    25981 gccctctccg tcacggcccg ccgcccgggc gtcttcggcc ggtgcggcgc gcagtcgacc
    26041 gggggcggcg acggcggggg attcgaggcg ctcctcgcag acctcgggag cctcccggcc
    26101 gcgagcgtcc gcgtccacct cgacgcgggg acgttcgaag cgctcttcca cggcttcgac
    26161 ctcctctccc tcacgcgccg cctccgcacc gctctctccc agcgccaggt cgtccagtac
    26221 cgagaggtcc ccgaggggca cagctggggc tcctggcgcg cccgcctcgc cgacgcgctc
    26281 acgttcttct ggggagacga gcggcccgcg ccgtcaccgg cctctcgccc gctcgccggg
    26341 cctgcgtccg gggccgcgcg gttctcgggc gagccggtct cgctcgacgt gaaggacgcc
    26401 gacctcgccg acgtcgtccg caccctcacc gacgggcgcg gcctctccgt cgtcgcgccc
    26461 ccgtctttcc gcgggacggt taccgcgtcg ctccgcgacg tcccgtggga ccaggccctc
    26521 gacctcgtcc tggcggggaa cggctggggc ttcgtccgcg aagggaacgt gatccggatc
    26581 gtccggcggg gtgaccccgg ccactgacgg cccttccgga cgctcagccc tccaccgtct
    26641 cccgcttcga gctgaggtag acgaacgccg cgagggcgag gaagacggcg aggccgagcc
    26701 agttccccca cgcggagagc cacgcgccga ctccggggac cgccgtcagg tcggggacga
    26761 gcgtccggcc ggtcgagtcc tcgacgaact tcaggagcgc cgcgaggacc ccgacgaggg
    26821 cgccccccgc gatgaacccg gaggcgatga gcgtcccctt ctcgtgccgc gccttcgcga
    26881 gaggctcgtc gggcgtcgac cgcttcacga gccaggcgac ggccgcgccg aggacaagcg
    26941 gcgagttcag ctccatcggg aggtacatcc cgagcgcgaa cgcgagaccc gagacgccgc
    27001 acatctccac gacgacggcg aagacggcgc cgacggcgta gaggaaccac ggggcgcccg
    27061 catcccccat cacgccgcgg aggaccgccg ccatcgcgtt cggctgcggc gccgggagcg
    27121 ggttctcgtg gagcggcgac ggggcgaagc cgtagacctt cgccatcagg aggatgacgg
    27181 ccgtcgtggc cgccgaggcg gcgacggagc cgaggaggtt ccagacctcg atccgccgcg
    27241 gcgtcgcgcc gagccagtag ccgatcttgt actgcgtcac gagggaggcg ctcatcgaga
    27301 gggccgtgca gacgacgccg ccgacgagga gcgtctggag catcccgctc tcgccggaga
    27361 ggccgagcga ggagaggacg acggcggtga cgatgagcgt cgtgagcgtc atccccgaga
    27421 cgggcgtgat cgagatcatc gcgacggccc aggctgagac cgccgcgaaa aggaacgcga
    27481 tgaggagcgt gaggcccgtg gagacgagcg cgaggcgcga ggcgcccggc gtcccggcga
    27541 ggacggagag gcgaaagtag aggaggatcg cgagcgccgt cgcgacgatc cccgccagga
    27601 cgacccacat cgggagcgat cggtccgtcc gctcggcctc cgccgccgcg ccgccgccac
    27661 gggcgaggcg cgcgacctcg ccgaacgcct gccgcgtcgc ctggacgatg acgggcgaca
    27721 tcttcaggat cgagaggagc ccggcgcaga agatgccgcc gatcccgatc ggcctcacgt
    27781 actcgaagaa gatgtcgtcc gcgggcatcg cggcgagagc cggcgccccg gccgagatcg
    27841 cccccggcac ccactgcgag agccacgcga agagcgggac gaggacgagg aacgagacca
    27901 tcgaccccgc catgatgatc gccgcgtagt cgagccccat gatgtagccg aggccgaaga
    27961 ccgcggccga ggtgttcatc gtgaagaccg ccttgaagcg gtccgtgaag ccggaaaggg
    28021 ccgggatcgc ggcggtcgag aagttctcgg cccacccttt catcgacggc cccacgaagt
    28081 cgaacaccgc cgcgagcgcc gcggaccagg agaggacgac ggcgctccgg ccgccccgct
    28141 tccccgccac gaggatctcc gtcgtcgcgc gcgcctccgg gtacgggagc ttcccgtgga
    28201 ggtcccgcac gaagtagcga cggaacggcg cgaggaggaa gacgccgagg accgctccga
    28261 gcagcgggac gaggaagatc tgccagaacg acgagcgccc ctcgaggccg aggatgtgga
    28321 tcgccggcat cgtgaagaca gagccccccg cgatgatccc cgaggtcgcc ccgacggcga
    28381 ggacgttcac gttctcgagg agcgtcgagg cgcgcgcggc gaggagcttc acgaagagga
    28441 ccgaggcgcc gacggcgagg atcgagatcg ggatcgtcga ctcgatcccc tgcccgagct
    28501 tcagcgcgat gtaggtcgcc gcggccgaga agacgaccga ccagaggaac ccctgcgcca
    28561 cggagcggac ggtgacctcc ggaacggacg cccccgcggg gacgaccggc tcgtacgtct
    28621 cgcccggggc gagcgtgcga aaggcgccgt cgggaagtcg gacgtcctcg aaggcggcgg
    28681 gggaggcttc gggcttcggc tcggccatct tgggatgatg ccgcagcgcg gggctcccgg
    28741 cgtcgtctgg tctaatcggc ccggaggttc ccgtgccgtc cgagaacgcc ccgacgcggc
    28801 gcgagccgcc cgtcctcctc gcgctcgccc tcgccgcgct cgtcgtctcc ggagtcgggc
    28861 cgaaggaccg gttcacctgg ctcctcgagg tcgcgcccgt cctgatcgga gcgccgctcc
    28921 tcgtcgcgac gacccggcgc ttccccctca cgccgctcct ctaccgcctc ctcttcctcc
    28981 acgccctcgt cctgatgctc ggcggccact acacctacgc gcaggtgccg ctcggggagt
    29041 gggcgaagga ggcgttcggc ttcgcgcgga accactacga ccggctcggt cacctcatgc
    29101 agggcttcgt cccggcgatc ctcgcgcgcg aggtctatct ccgccgctcg ccgcttcggc
    29161 cgggcggctg gctccgcctc gcggtcacgt ccgtctgcct cgccttcagc gcgctctacg
    29221 agctgctcga gtgggccgcg gcgatcgcga cgggagaggc ggcgaccgcc ttcctcggga
    29281 cgcagggcga cgtgtgggac acgcaatggg acatgttcct cgcgctctgc ggcgccctcc
    29341 tcgcgcaggc gctcctcgcg cgcctccacg accgccagct cgcgcgcctc gccaccgcct
    29401 gaggagcgca acaaggaaaa gggccgggcg cccgaaggcg tccggcccgg tctcgcgcga
    29461 ggcgcgaagg gtcttacttg gcgccggggt tcgtgccggc cttgaactcg tcgaggttcg
    29521 acttgccgtc gccgtcgctg tcgagcggct cgagcttcgc ccagtcgaac gtcttcttct
    29581 cggccttcgg gtcgacgacg aacttcatca tgtccttgcc gaacgcgttc acctcggcgt
    29641 cgcccttctt ggccatcgcc ttcacgtggc agctggcgca cttggccttc gccgtggggt
    29701 acttcgcgtt catgtccttc tgcatgtcca tcgtggcgac ggcggtcgag ccgagcacga
    29761 tcgcgaggac ggcgacgagg aggacggcgg tggtcttctt catctcgtgg ctccttttgg
    29821 tcggggctga gccccgttcg gattatccct tttcaagttc tctgccatcc ggtaaagggg
    29881 acgattcggc gctcccagcc ctcggaactc cctggcgcga cgcatcgaga cggcgtcgcg
    29941 gaagcagccg agcggggtgc cacgcccaac ccggacgagg ccgacgacga agaggtcgtc
    30001 gccggcctgc cggtcgcctc tacgcggtgg ccgggacgat cgagccctcg gtcgggcaga
    30061 cctcgacgca cttcggcgag tcgtagtgcc cctggcactc cgtgcacttg gcggcgtcga
    30121 tgacgtaggt catgtcgccc gggctgatcg cctggttcgg gcactcgggc tcgcaggtcc
    30181 cgcagctgat gcagttctcg gtgatcatca tcgccatgac ggcctcctcg tttccggggg
    30241 acgccttctc cctgtccccc gcggagccgg accacgaacg cgggccggcg gccggaagag
    30301 gctaggggag gtccgtggcc ggcacaatga tgcgaacgga tcattccgaa atggcgcttc
    30361 cggagccgcg tatcccgttg acatcacgcc ggatcgggtt ccaaattccc tgtggtggcc
    30421 gctcgcccga aggaccggca ggaggtcgtg gcggcagtgg cgtcggcggg gccggcggcg
    30481 gaaggagccg ccgggagaga gacggagagc gtcggcgtcc gacccgttcc ggaagcacga
    30541 aaggagaccg aagtgagagt tccgacccga ttcctcctcg tcgccctcgt cgccctcgtt
    30601 ctggcgccgg ccctcgtcgc ccaggaggga ggccgcatcg acaccgccat cgcgtccgac
    30661 agccgcgaga cgatggccga gcgctcctcg ttccccccga cgcagccgaa gatctacgtc
    30721 ttcgcgatgg tcgccgacgc gccgccggac acgccggtcc gggccgtctg gatcgcggag
    30781 aagtgcgaag ggcagccgcc gaacacgaag ttcgcggaga ggacgacgaa gttccccgga
    30841 ggcgcgacct ggatcccctt ctcgtacacg aagccgaatc cgcagtgggc gtccggcgcc
    30901 taccgcgtcg agctctacct cggcaagcag ctcgcggcga cggtgaagtt ctcggttacg
    30961 aagtagcgcc tcccggcatc ccggtatccg cgtgcggccg cccctccggg gcggtcgctt
    31021 cgctttgtgc ccctccccgc agctccgcgg ctccgcccga gacgatgaag gccatcgcat
    31081 cggccccgcg cgcggtgacc ggtgtcacga ggcgtcgcgg gacgacgagg agctggccgg
    31141 cgaagttgta cgactgcggc aggtagacac cgacgtggtc ggcgaggccc gcgccgtcga
    31201 gcgaatcgcg ggtcaggaag ccgaccgcct tgacgccgct cgcttcgtcg agcgtgacga
    31261 agaccggccg ctcgaacccc ttccgctcgc cgacgaacgc gtccgtcacg tcgcggatcg
    31321 ccgagtagag gagcttgacg aacgggaggc ggcggaagac accctcgacg agctcgaaga
    31381 cgcgcttcgt gacgaagttc gaggcgagcg ccccgacgac gacgatcagc gcgatcgtgg
    31441 cgaggaaccc cgctccgggg acggggagcc ccatccacgc gtcgaccgcc cggaagacga
    31501 agacgacgat cgcgatcgtc aggaccgacg ggacgaagac gaggagcccc tggaggaagt
    31561 atcggaggag gacgcgcatg gcgcggggag tctacggggc cgggcgccgc cctgccgccg
    31621 gcccggggcc cgggtcgcgc tagcgcggtt tgcgccccgt cccgtggtag aaccagctca
    31681 gcccggaaag cgaaccgagg aggagtgccg tgcccgagac gttccgctcc gtcccgcaga
    31741 tgctgcttcg gcgcgtcggg aagacgcccg accgcgaggc ctacctccgc cccgtccctg
    31801 acggctggga ggccctctcc tggcgcgagg tgggcgacag ggtccgtgcg atcgcgtgcg
    31861 gcctccgcgc cctcggtctc gaggacgagg agcgcgcctc gatcctcgcg agcacccgcc
    31921 tcgagtggat cctcgccgac ctcgggatcc tctcggccgg cggcgcgacc acgacgatct
    31981 acccttcgac cacgacgccc gattccctct tcatcctcca ggactcggac acccggttcc
    32041 tcttcgccga gaacccggag caggcggaga ggatcgcgcg cgagcgctcg aaggtccccg
    32101 gcctgaaggc gatcgtcctc ctcgaggggg aggcggccgg ggacggcttc gtcctgacga
    32161 ccgaggacct gatggagaag ggacgcgcct gggacgccgc gcatcccggg cggttcgaag
    32221 cgctcgtcga ggccgtccgc cccgacgccc tcgcgaccct catgtacacc tcggggacga
    32281 cgggccggcc gaagggagtc gagctgacgc acgactgctg ggtctacgag gcggagggga
    32341 tcgacgcgct cggcctcctg acggaggacg accggcagtt cctctggctg ccgctcgccc
    32401 actccttcgg caaggtcctc gaggccgcgc agctccggat cggcttctcg acggccgtcg
    32461 acgggcgcgt cgagaagatc gtcgagaacc tcggccaggt ccggccgacg ttcgtcgcgg
    32521 cggtcccgcg catcttcgag aagatctaca acaagatcgt cggcggcgcg aaggaggccg
    32581 gcggcgcgaa gtgggcgatc ttctcgtggg ccgtcggcgt cggccgcgag gtctcgaagc
    32641 tccgcctcgc cgggaaggag ccggccgggc tcctcgccct gaagcacaag atcgcgcaca
    32701 agctcgtctt cagcaagctc caggcgatct tcggcgggcg cctccgcttc ttcatctcgg
    32761 ggagcgcgcc gctcgcgaga gccctcggag agttcttcca cgcggcgggc gtcctcatcg
    32821 ccgagggcta cgggctcacg gagacgagcg ccgccagctg cgtgaacacg ttcgagcacc
    32881 tgcgcttcgg aacggtgggc catccgctcc ccgggaccca ggtgaggatc gccgaggacg
    32941 gcgagatcct catcaagggg cgcggcgtca tgcgcggcta ccacaacctc cccgagcaaa
    33001 ccgcggaggc gctcgacgcg gacggctggc tccacacggg cgacatcggt cacgtcgagg
    33061 acggcttcgt gaagatcacg gaccggaaga aggacctcat caagacctcg gccggcaagt
    33121 acgtcgcccc gcagatgctc gagcgcaagc tcgccgtcgc ctgcccgtac gtgagccact
    33181 cgctcgcgca cggcgacaac cggaacttcg tctcgatgct cgtcgcgctc gacccggagg
    33241 cgatcacgaa gtgggccgcc gccaagggcc tcggggggag gccctacgcc gaggtcgtcg
    33301 cctcgaagga ggtccgcgac ctcatcgcgc cctacatcga cgaggtcaac gcggacgtcg
    33361 cgagctggga gcaggtgaag aagtggacga tcctcccggc cgacctgacg ctcgagacgg
    33421 gggacctgac cccgtcgatg aagctgaaac ggaaagacgt cgagcggaag taccggcaga
    33481 cgctcgacgg cttctacgag gggacggtcg tcgacgtctg agccgggccg gagcgcaccc
    33541 gcccggcgcc tcgccgggcg gcgcgctcag ccccgcgtca ccttctcgag gaggaacacg
    33601 tacgaccggt agacctcgcc ggtcgcccgc gtcatcccga tctcgcaggt ccggctgctc
    33661 gagaggtgct cctcgcaccc tttcgccttc acctcggccg cctcgtgctt cgtcgccgag
    33721 gccgtcagct cggggtggag gaacccccgg tcccccgcga aggcacagca acccgccgag
    33781 agcggcacgg tgacgtcgtc gctgcagaag cgggcgatcc cttcgagctt cgcgccgagg
    33841 ttcatcttcg tgagcgagca gaccgggtgg agcgcgacgg ggccggcctt ccgcttcgcg
    33901 gtgagcttcg gcaggagcgt gtcgtgcgtg aaggcgacgc cgtccaggat cgtcagccgg
    33961 tcgaaccgcg cctggttctc gggcgtcagg ttcgggcggc tccccttcac gccgtacgtg
    34021 caggggctcg tgtcgacgac gaccggcaag cgcccctcgt ccgaccaggc ccagaagcgc
    34081 tcgatcgccc ggttcgcggc gaacgcgttc gcctccgtgt accccttcga cgagaaggga
    34141 acgccgcagc agacgccttc gacgtcctcc gggatgaaga ccggcgtccc ggcccgctgc
    34201 gcgacggtga cgaacgcctc catgagcgaa gtctcggaag gctcgccggg gagcgccccc
    34261 atcgtccgcg agatgcaggc cgggaagtag accgcggccg cgcccgcctt cgtcgtcgcc
    34321 gggcgcgcgg ccttcgcggc gcgcggcatc tcggcgctcc actgcgggaa cggcttgccg
    34381 acgaccgccc ggatcgcgcg cgtgatcccc gccatcgcgc ccgccccgag cgccgactgg
    34441 acgaggtgcc ccgcgcggag gcccgtccgg aggagcggct cgaccgtcgc gaaccgtcgg
    34501 gcgatcgcga gcgcggcctt ccgcgcccgc gccgagtggc gcgccttccg gagacgcttc
    34561 gtcagctgcc ccgtgtcgat cgagacgggg caggcggtgg cgcagaggcc gtcgacggcg
    34621 cacgtgtcga ccccggcgta cgggaactcg gactcgagcg ccgccaggag cggcgcgttc
    34681 gtcccttccg cctcgaggcg ggtcatctcg cggcggagga cgatccgctg ccggggcgtc
    34741 agcgtcagct cgcggctcgg gcacttcggc tcgcagtagc cgcactcgat gcacttgtcg
    34801 acctcttcct cgacggtcgg catcggcttg acgtccttca ggtggcagag cgggtcggcg
    34861 ttcaggatga cgccggggtt caggatccct tcgggatcgc agagcgcctt cagccgcttc
    34921 atgacggagt aggcctcggg gccccactcc gtctcgacga acggcgccat gttccgcccc
    34981 gtgccgtgct cggccttcag cgcgccgtcg tacttcttca cgacgagctc gacgacgtcc
    35041 tcgatgaggc gggcgtactt ctcgacggcc gcctggtcgt tgaacgactg cgtgatgacg
    35101 aaatggaggt tcccgtcctt cgcgtggccg aagaggatcg cctccgggta gccgtgcttc
    35161 acgaagagct tcgtcaggtc gacggcggcg tcggcgaggt gctcgatcgg gaacgcgacg
    35221 tcctcgatga ggaccgtcgt cccgctcctg cgggccgcgc cgacggaggg gaacgttccc
    35281 tgcctcacct tccagaggag cgcctgttcc gtggcgtcgt gcgtgaagcg ggccggctcc
    35341 aggagggcga gcggcccgac ggccgccgcg gcgacggcct cgagcccctt ccgctcggcc
    35401 tcgtcgcccg cctggtactc gacgaggagc gccgcggcgc cctcggggag cgtccggatg
    35461 gagggcggga cgcccggctg cgactccacg gaacgaagcg aggcgcggtc gaggagctcg
    35521 agcgccttcg cacccgagtc gcggagcggg acgatcgcgg cgcaggccgc gtgcatcgag
    35581 gggaagagga gaagcccggt gtacttgacc gggaggtcgg gaaccgtgga gaggaccgcc
    35641 tcggagatga acgcgagcgt cccctccgcc ccgacgagga ggttccggaa gatctcgacg
    35701 ggc
//
LOCUS       DAOONL010000028        35594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_952_length_35594_cov_18.043248, whole genome shotgun sequence.
ACCESSION   DAOONL010000028 DAOONL010000000
VERSION     DAOONL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 35594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35594
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_35594_cov_18.043248"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1296)
                     /locus_tag="PLP50_06460"
     CDS             complement(337..1296)
                     /locus_tag="PLP50_06460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51227.1"
                     /translation="MTSPPTVPRKVSVVVPVYNEEANLPRLLPRLVPVLEGLGRPWEV
                     VFVDDGSRDRSLELLKAAVAERPGRVKAVELLRNAGQHMAIMAAFGETDGEYVITLDA
                     DLQNPPEEIPKLVGKLDEGFDVVGTIRSKRRDSLFRRAASRIVNRTTGLLTGMRLNDY
                     GCMLRGYHRDVVDVMTASDEVSTFIPALAQLYARRPVEIDVAHEERAEGESKYSLLRL
                     VRLNFDLMTGFSLVPLQLFGPLGFVTALFGVGFGIYLLVRRLVVGSEVEGVFTLFALA
                     FMVMGVIMAGVGMVGEYVGRIYQQVRGRPRFLVRLVHGGRREP"
     gene            complement(1373..3112)
                     /locus_tag="PLP50_06465"
     CDS             complement(1373..3112)
                     /locus_tag="PLP50_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA51228.1"
                     /translation="MGAYLTSGSRFRSEPLPPASRALLWTLLILAVAVLVADTGHTPF
                     FEPDEARYAEIPREMLATGDLVAPRLNGFRYYEKPPLHYWAVAASMSLFGESESSARL
                     PVKLSAAGMVLVTVLFARRRYGERVALVAGLVVATSGLVVALARLNIIDGPLSFALSS
                     AAFAFAAFQEHERSGDRARARIALYLLHFACAAAVMLKGFVGLVLPGGAILVWALLSR
                     RLGIVPKLFSPGPLLLFLVLVVPWHVAMARRDGEWFDFYVVGEHFRRYFETGHRRTAK
                     PFFFVLVLLGGLVPWTPFLGRLAGAFPSLRRGDWRERGAEAYLQVFWILVLVFYSASR
                     SKLVPYLLPIWPAVAVLLALGLERARQRGAALRGERRAAGLLFGLLAGATAAYAFGAG
                     LAARYGVEVQAAVAVGSLALGALLNLLAGRSAARAPRRAFSTERFALGMVLPWLGFSG
                     ALVLALPSVARSITPWPLVATLKRELRPDDLLLQRGHYLQGPAFYTKRLTPVADLPWS
                     ELDFGKEEARRRGLYLSDEEFARKWHGPARVLCIVHFGHLRDFGNPAAGLSLPHVLAR
                     SPNGKFLLVSNRP"
     gene            complement(3097..4533)
                     /locus_tag="PLP50_06470"
     CDS             complement(3097..4533)
                     /locus_tag="PLP50_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51229.1"
                     /translation="MGHGGGDLRRGLRRRIRLLRPAARLVPGGGLSPRARSAVEARDL
                     SKVYRRWGRTRAFGTLKSALLGRGLGRALEPSETVSALSGVSFDVRRGETFGVIGANG
                     SGKSTLLKLVAGLFKPTSGTLRVDGKVSALIELGAGFHPEISGRENVVINGVMLGLTR
                     KEIERKLPAIVEFSGLSEFIDEPVKTYSSGMYVRLGFAVAVHVDPEVLVVDEVLAVGD
                     EAFSRRCLDTIKEFSTRGRTIFFVSHSLALVEELCDRVLYLSGGRVAGLGEPREMLAA
                     YRLDVAAGEGTRLLAEHAVGQEALRAEAAAPVPEPAGAAEGDGLEAGPAPAERARRWG
                     DRSATIEGVRLLDRDGLERYAFRTGEPVTIEVEAAPARPLDDFVFGVGLFTPEDVCVH
                     GSNTEIDGFAAGRFDAAAKVRVTIPRCDLGPGTYLVDVAIHARNGTPYDYWRGACRFR
                     VDSPRTEAGVWAPERSWTVEGGATWART"
     gene            complement(4440..5246)
                     /locus_tag="PLP50_06475"
     CDS             complement(4440..5246)
                     /locus_tag="PLP50_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51230.1"
                     /translation="MERLRDLWRHRALVGVLVTREVKARYRGSVLGFFWSLLNPLLML
                     AVYTLVFQLLLPNRSPSTSPYALFLFCGLLPWNWLSSAITDAASSLLTHGALLRKILF
                     PAEVLPAVAVLAQGVHFLLALPVLLAALVAGALGAFGAKVPLGWPLLQVIPLLVLQGL
                     LLLGIGLFLAALTVHFRDVKDLLQTVLSVLFFATPILYTLNDLPKTKLTRLLALNPFA
                     PLFAGWHDALFYGRVSPPQTWATAAAISVAAFAVGFVFFDRLRDSFPEAV"
     gene            5321..6010
                     /locus_tag="PLP50_06480"
     CDS             5321..6010
                     /locus_tag="PLP50_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51231.1"
                     /translation="MGARVEVVEVRKSWGAGGSGGDAASAEALAGVSFVLDPGEFVAL
                     LGPSGCGKSTLLHLLGGLDRPSSGRVIVDGSDLARLSEADRDLYRRRTAATVFQLFNL
                     LPTMTALENVALPLLLDGVPASEADGRAERLLGEVGLAAKSGSFPWQLSGGQMQRVAV
                     ARALVASPALLLADEPTGSLDSRSGAQVLDLIAELASARRVTVLLATHSDEAARRASR
                     TLRLRDGRLER"
     gene            6007..8598
                     /locus_tag="PLP50_06485"
     CDS             6007..8598
                     /locus_tag="PLP50_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPA51232.1"
                     /translation="MSRTRPLLAPLLLRPFREDRGRFVLTVAGIAVGVATMAAILLAN
                     ASVLSSFTATVDVVAGKAGLTVLADGPGIPEETLERLAWLRMKGVAIVPAVSVTVALA
                     GGEGDVLELLGIDPVTDPSVRDYRFVAETADETSLEKLFGIFRAGAVLVPAPLAERLG
                     LGPGAELGVLSGGAAHRLRVAGVLRMEGAARAAAGSVLFADLATAQEVLGREGFLDRI
                     DLVLPAGLADADRTALEASVRDSLPPGVTVGRPERRTETVDRMVRSFRVNLTALGLVA
                     LLVGTYFVYNTLSISVLRRRSDIGTVRSLGASARSVFLVFLAEGAALGALGGALGVAL
                     GALLARVALAAVGGTATDFYLPQAAPVFTLAPSALATSFLAGLGTSILSALAPAVEAA
                     QVEPASTLRHGSVEASRRRAVGPLALGGVAALALAALATRPGPVDGLPLFGFASVFLV
                     IAGVSLLAPLSVVTVARLADGPAVRLLGVEARIARANLTGSLSRTAVAVAALSMGISM
                     MVAVAFMVGSFRATVVDWVSQTLKADLFVAPESGRSQRALGRLPEEAIRLVEAVPGVA
                     SVDPFLSFEATRDGAPYTIGSGRFDLVAAHGDLPMADGSDTRKVLARALARGEAVVSE
                     PYSVKFGVNAGDTVTLPALDGPVALRVAGVYRDYSNDRGTVTIDRALFRRLFRMDGAG
                     SLAVRLEPGVAPEEARRRLLSAADGRFALRVQTNAALREAALALFDRTFAVTWALEAV
                     AIAVAVLGVFSTLLALVLERRREIALLRVLGASAARVARAVRLEAAALSSLGVVLGVA
                     AGAAMGIVLVHVVNRQSFGWTIRTDVPWLFLAGALALVLLATLAAASRPARLAAATDP
                     AAVLKEE"
     gene            8603..9775
                     /locus_tag="PLP50_06490"
     CDS             8603..9775
                     /locus_tag="PLP50_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HPA51233.1"
                     /translation="MSPCRAPVRVRPVRELVASLRPAAALAALSLLFSFAPLAGEGGR
                     FRPLDPKATPAFPRDHGAHDDARVEWWYVTGHLVDRDGRRSGFQLTFFRTGLVDEPKG
                     ARSSAFAPRDLHLAHFARTDVDGRAFRYAERTHRDGPGGASARTTHLDVANEDWRLTE
                     LGGKLYLRASDRVDGVEETLTLLLTPEKPPVLHGENGLSKKAAEPDAVSRYVSFTRLR
                     AEGWATRGDEARSVTGSAWMDHEWGSGAIGRGTRGWDWFSVQLEDGRDLMIYQLRGED
                     DTRTLHSSGTLVERDGRATPLAPGDFSVEVTARWTSQRSKGTYPARWLLRVPKASLAL
                     EVVPVLPDQELVTDRSTRVTYWEGACDVRAPGAGRPLGRAYVELTGYAGKGALGLF"
     assembly_gap    10049..10148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10149..11410)
                     /gene="rseP"
                     /locus_tag="PLP50_06495"
     CDS             complement(<10149..11410)
                     /gene="rseP"
                     /locus_tag="PLP50_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPA51234.1"
                     /translation="MQISTNILAFVFVLSFLIFFHEFGHFLVARLFKFPVEVFSIGFG
                     KRLFGFKRNGTDYRVSLVPLGGYVKIIGLGPDESDVVDAGADQGLRGTRWQRFLVLLA
                     GPAVNLVLALLLTAGAFTIGVTVPKYAEERPVVKHVDAGSPAEKAGVRIDDVVVSLSG
                     KPVSTWREVETHLGLAPRAEIPVVLERGGESVSVVIRPEPQTKYDIGYTGLNPWLAPV
                     IGSLVKGYPGEKAGLQVGDRIVSIGGVAIDGYFEVVRRVREASSAFEEGKPQPISFEV
                     DRNGTRVALDVTPRFEGGSWRIGFTPKYELVERKMPFLAAIGASWKENLRQTSAVGQT
                     IKRMVSGSGSIRQLSGPVDIAKFSGEAVRTGWAALLGFMGLLSLQLGLLNLLPIPLLD
                     GGQLMVLTFEGIFRRDFSLKFKERLLQVG"
     gene            complement(11423..12622)
                     /locus_tag="PLP50_06500"
     CDS             complement(11423..12622)
                     /locus_tag="PLP50_06500"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPA51235.1"
                     /translation="MNAPRGVALLGATGSIGRSALSVFEALPERFRVVSMSAGSNLDA
                     LLPAIARFRPTVVSVKERAAADRVRREFPGVRVGFGEQGMIDVATHPEAEVVLGAVVG
                     AAGLPPAYEAVKLGKTLALANKEVLVVAGEAVMAAAAASGSAVLPVDSEHCALHQALR
                     CGRPEEVDRLVLTASGGPFRKRPLETFDAITIEDALAHPTWKMGPKITIDSATMMNKG
                     LEVIEARWLFDVPPERIGVVIHPQSIVHSFVEWIDGSVIAQISPNDMRFPILYALTYP
                     ERVPTPMPKLDLPSLGTLELAALDERRYPAVPLAYAALRAGGTAPAVLNAANEVAVAA
                     FLEGRIPYRSLVPVVERVLADHPVSPATTLEAVLEADREARRRAAELVSSGAPLASPR
                     TVAPASA"
     gene            complement(12619..13440)
                     /locus_tag="PLP50_06505"
     CDS             complement(12619..13440)
                     /locus_tag="PLP50_06505"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPA51236.1"
                     /translation="MRRTRELTAAVLIPLVLLVLVKGPLLSWAVLAGLACAAALHEFH
                     GLIRCGGWLAPRALSFVLFATIVVAAYLGSAPLLVAAAGAVLVAVPSAVLLAAPAEPV
                     WLASSATTSFATLYVGAGAAAIVALRGIGWEPVVFLLGIVWAGDSAAYYAGTRFGKRR
                     LAPVVSPKKSWEGFWGQVIAGALFGALLALVLPRIPGEPPAVVAGGALLGAVLSVVAV
                     VGDLVESTFKRSCSVKDSGGLLPGHGGLLDRLDSLLFASPVLFGILALLPELRPR"
     gene            complement(13437..14261)
                     /locus_tag="PLP50_06510"
     CDS             complement(13437..14261)
                     /locus_tag="PLP50_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPA51237.1"
                     /translation="MIEKDLVAAPGTPERALLDTLDLGRLPRHVAVIMDGNGRWARQR
                     HLPRVEGHRAGASAVRETVETAARLRLDALTLYAFSTENWKRPKAEVSTLFALLKEYL
                     RREFRSLVENDIRFRVVGRPDGLDPSVRSALSTALEATASCRGMVFSVALNYSGRAEI
                     ADAARALARDAAEGRLDPESIDEAAVARRLYTADLPDPDLLIRTSGELRISNFLLWQI
                     AYAEIHVTPVLWPDFRRRHLLEALLDYQRRERRYGGVTEEDASAPGAPPAPAALGA"
     gene            complement(14312..15103)
                     /gene="truA"
                     /locus_tag="PLP50_06515"
     CDS             complement(14312..15103)
                     /gene="truA"
                     /locus_tag="PLP50_06515"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPA51238.1"
                     /translation="MSVRGEVSHKAGAEGARRRLELSYLGTHFEGWQAQDVVRTGTGP
                     PRTVQLVVEEALSKMHGSRVRVHAASRTDAGVHAEGQVAHFDVPPGGPLVPREGLRRG
                     LNALLPWDVRVEDAAEVPFAWHARFDALGKRYVYRLRRADHLPPFEGLREALASPRLD
                     VRAMRAAAARLVGRNDFASFGLAGAPRRTTVRTLARLDVGELGPLVVVTAVGDGFLRG
                     MVRRLVGTLVEAGLGRTDPGEAFRRPGPTAPARGLTLEKVFYRAG"
     gene            complement(15227..15472)
                     /locus_tag="PLP50_06520"
     CDS             complement(15227..15472)
                     /locus_tag="PLP50_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51239.1"
                     /translation="MILVRNRNGTLYFLARDGDDALVSLRRSYPGRPLAILTKGDGLA
                     IVRVGDPLPYGEGGTEERLEIVNVVLPPAPEAFSFRA"
     gene            15624..18233
                     /gene="clpB"
                     /locus_tag="PLP50_06525"
     CDS             15624..18233
                     /gene="clpB"
                     /locus_tag="PLP50_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPA51240.1"
                     /translation="MNPDRLTLKVQELLQVIARDARSAGNPEVTPEHLAAALAAEPSV
                     GTPLLTRIGAPPALLAGELGALVARLPKVQGSGAETRFSPALVRILDRAEATAREFRD
                     EYVALEHLLLALLRDGASKAAEILRRHGVTEAAVLSALKEVRGSARVDDPNAEEKYQA
                     LKRYARDLTELARRGKLDPVIGRDDEIRRVMQVLTRRTKNNPVLIGDPGVGKTAVVEG
                     LARRVASGDVPESLKDKQVVALDLGAMVAGAKFRGEFEERLKAVIKEVEESEGKVILF
                     IDELHTLVGAGAAEGAMDAGNMLKPALARGELRAIGATTLGEYRKHVEKDPALERRFQ
                     PVVVGEPSVEDTISILRGLQERYEVHHGVKITDAAIVAAAVLSNRYIADRFLPDKAID
                     LIDEAASRLKMEIDSLPTEIDEVERKILQAEIERKALEKDDSAPARERLSALEKALAE
                     LRERSLSMKAVWKNEKDAIGAIRDLKKKTEDAKADAERAERQGDLSRAAELRYGVLNQ
                     LDRDLAAATAKLEELQEDGGFLREKVEEEDVAAVVSRWTGIPVSKMVEGETQKLLAME
                     ERLRLRVVGQDEAVTAVSAAVRRARAGMNDPKKPIGSFLFLGPTGVGKTELARALAEF
                     LFDDEGAMVRLDMSEYQEKHTVSRMIGAPPGYIGHDEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPDVWSTLLQVLDDGRLTDGKGRVVDFRNVVVVMTSNVGSQLIQELQGVDEEEMR
                     KGIEGELRHRFRPEFLNRIDDVIVFRRLTLEEIGKIVDLQLARVAALVAPRGVRLEWT
                     DAARRLLAERAFDPLYGARPLKRNIQKLVQDPLALRILAGGVPSGSTVTLDAGAPGEG
                     VAFSIGSPAAPAA"
     gene            18257..18733
                     /locus_tag="PLP50_06530"
     CDS             18257..18733
                     /locus_tag="PLP50_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPA51241.1"
                     /translation="MAAVRRYDPFRELATLQERMNRIFDDTFSSASRREDEGLSATWT
                     PPVDVLEKRDRIVLTAELPGFAEDQVQLRFEDGVLTLEGERRFEKEVDEARYHCVERS
                     YGRFSRSFRLPANVDEESITATFVNGLLVVELPKREEARPKSIRIRAGSPPAIDVK"
     gene            complement(18744..19700)
                     /gene="cysK"
                     /locus_tag="PLP50_06535"
     CDS             complement(18744..19700)
                     /gene="cysK"
                     /locus_tag="PLP50_06535"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPA51242.1"
                     /translation="MRIASDVTKLIGNTPLVRLNRMAKDAAPGAVVAAKLEYFNPANS
                     VKDRIGVAMIEALEKEGRIAPGKTTLVEPTSGNTGIALAFVAAAKGYRLVVAMPESVS
                     LERRKVLRLLGAELILTPAAEGMKGAIARANERLAELGENGVMPQQFENAANPAIHEA
                     TTAVELWNDTDGAMDVFVAGVGTGGTMTGVGRYWKPKKPSLRLVAVEPVHSPVLSGGA
                     PGPHRIQGIGAGFVPANLDTALVDEVVQVTNDQAFEYARRLAREEGILGGISSGAAAF
                     AALEIARRPDSAGKLVVFVVPSTSERYISTDLFKEDEAGPAA"
     gene            complement(19732..20112)
                     /locus_tag="PLP50_06540"
     CDS             complement(19732..20112)
                     /locus_tag="PLP50_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51243.1"
                     /translation="MVEIGSDPERDRGAAELAGLLALSRESVHQLLAPYVRAGLLVAV
                     RGRAGGYRAGEGLLGSPLSAVLAPFGAPSGRGRAAAGKGLSRLVEAVESEAVAARLAV
                     YERWTVGDLVARHRADRHVIDWEI"
     gene            20201..21517
                     /locus_tag="PLP50_06545"
     CDS             20201..21517
                     /locus_tag="PLP50_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPA51244.1"
                     /translation="MSRHTLAAAALLLSASTALAQRGGSEVRRAEDDPRRSSIVRAVE
                     QIGPSVVNISAERLVQRRATPFQEYFGVPGDRGRRSESLGSGVVLDASGIVVTNDHVI
                     SGASRIFVTTSDGRELEAEVLGADADNDLAVLKVDGKGLKPVRLGATGDLMIGETAIA
                     VGNPFGLSNTVTTGIVSALQRTVRGETRTYTDFIQTDAAINPGNSGGALCNVLGELIG
                     INTAIVGGANTIGFAIPVERVRRIVDDLLRFGEVKPVWVGIRGTTVTPERGRPSSRGL
                     GMRVRSVYPASPAEEAGLEPGDVVVSLNGRPVESREDFDTLLASVPPGQSVSVEMRRS
                     GELRKLSLAVARAPEDLGLEVLRREIGISVAQARGGLFLTSVARESPADRKGLERGDS
                     LLAVNGKRVSTLDDVARAVERGWGRSGLVLVVGRAGYAYTLTFGLE"
     gene            complement(21567..22109)
                     /locus_tag="PLP50_06550"
     CDS             complement(21567..22109)
                     /locus_tag="PLP50_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(21900..21902),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPA51245.1"
                     /translation="MRRLATLAAAVLLVAAPLAVAEEPKPLAAGDAAPAFSLPGSDGK
                     THALADYVGKKAVVLAWFPKVFTGGUTLECKSLRESGEELRSFDVAYFAASVDPLEQI
                     TKFAKSLDADYAMLADAEGSVAKAYGVLHPEKGYPRRWTFYIGKDGKLLHVDREVKAG
                     TAGPDMAKRLAELGVAKTGK"
     gene            complement(22180..23091)
                     /locus_tag="PLP50_06555"
     CDS             complement(22180..23091)
                     /locus_tag="PLP50_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA51246.1"
                     /translation="MSAPDLPRREALLADSALVLVTAIWGSTFVVNELVLKETPPLLF
                     LALRYGLAAAVLVLLARGRPRTPGVLRDGALIGLLLAVGIGCQLVGQLFTTASKAAFI
                     TGLSVPLTPVAAWLFHRQVPSGTNLAGLVLAAGGFGLMSWPKDASGLDPGDLLVFFTA
                     LVYAVIIVELGVASPKHDVRVYSAAQIVFAFLGVLAGRLLLTPFLDRPEAFFAAEARP
                     LAFTPQLLLAVLWMGLVASVVTSLVQTWAQARMPAVHAAVIFALEPVFTALFAAVALG
                     ERLSAREAWGGGLVLLGIVVSELRLRK"
     gene            23144..24571
                     /gene="gltX"
                     /locus_tag="PLP50_06560"
     CDS             23144..24571
                     /gene="gltX"
                     /locus_tag="PLP50_06560"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPA51247.1"
                     /translation="MNPAPRVRFAPSPTGSLHVGGARTALYNWLFARRHGGTFILRIE
                     DTDVDRNKPELVDQILEAMTWLGMTWDEGPFRQSERYDLYRQAAEKLLAEGKAYRAFE
                     TPEELEAARKAAEAQGETFRYSGAGREIPKAESDRRAASGEPFVIRLKMPDEPIVVDD
                     VVQGRAEFPAGALDDFVLMRSDGHPLYHFSVCVDDVAMGITHVVRGVDHLANTPKHVA
                     LFRALGAEPPKFAHLGMILGPDGKKLSKRHGAAGAEEFRAQGLLPEALVNFLALLGWS
                     PGEDRERMTMDEMAALFSLERIGPSPSRFDHEKLAWLNGQYLQDVPDARLAELVKPFG
                     VPGSCPDETLLRAIPLQKPRAKTLVELAQGLSSWVADPADWDAAGLKKFGKPAHAPLL
                     KTFLARIAAVTPLTHAGIEEAARALAAEAGVKLGDLAQPVRLALTGTLASPPLFEMIE
                     VLGLETTRRRVLALAARIEPEPTPS"
     gene            24580..26280
                     /locus_tag="PLP50_06565"
     CDS             24580..26280
                     /locus_tag="PLP50_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPA51248.1"
                     /translation="MEETTTPAPETAGKDFIREIVEDDLATGKHAKITTRFPPEPNGY
                     LHIGHAKSICLNFGVAREYGGTCNLRYDDTNPLKEDVEYVDAIEADVRWLGFDWHGKY
                     YASDYFGKLYDFAEELIRRGKAFVCDLTADEVRATRGTLTEPGKNSPYRDRPVEESLD
                     LFRRMRAGEFEDGSRTLRAKIDMASPNINLRDPALYRIRKAHHHRTGDAWCIYPMYDY
                     AHALSDWIEGITHSLCTLEFEDHRPLYDWCLLALGLESRPRQIEFARLNLTYTLLSKR
                     RLLHLVTGGHVRGWDDPRMPTIAGLRRRGFTPEAIRDFAERIGLAKRDSVVDVALLEH
                     CLREDLNRRAPRGMAVIDPVKVVLTNWPEGKVEELEAVNNPEDPSAGTRKVAFSRELF
                     IERDDFREVPPPKYHRLFPGSEVRLRWGYIVKCEEVVKDASGNVVELLCTYDPTSKSG
                     EAGAARKVKGTIHWVSAAHAVPAEVRLYDRLFTKEDPNDAPEGQDWLTNLNPASLEVR
                     QGFVEPALVGAAPGAFFQFERLGYFSVDPDGTPGKPVFNRAVGLRDTWAKIEKKGRTA
                     "
     gene            26334..26732
                     /locus_tag="PLP50_06570"
     CDS             26334..26732
                     /locus_tag="PLP50_06570"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPA51249.1"
                     /translation="MSKAPHGRRLLLWLPVALMAGVQLWLSSRSRLPDALSPFPGSDK
                     LAHGGWFFLLALLAWRAAREGEGWSRRRRLGGLLLAAALWAASDEAHQWFVPGRAVEA
                     ADLLADVAGAALALSLAEGFLTGRRLVRAG"
     gene            complement(26772..26845)
                     /locus_tag="PLP50_06575"
     tRNA            complement(26772..26845)
                     /locus_tag="PLP50_06575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26811..26813),aa:Gln,seq:ctg)
     gene            complement(26903..27442)
                     /locus_tag="PLP50_06580"
     CDS             complement(26903..27442)
                     /locus_tag="PLP50_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51250.1"
                     /translation="MTVDADRLRQVVESLLKGDKSLNEKDVKAAAGKQLKVAPEEIGA
                     GSIREVRRKLGIDRPAALAWARAQLVKAPTTEARVVIEAVAEKFGIRLGPPDVTRLRP
                     KEARRPRKNGRKKAGRAKVLKPEVVDAAPKKGRGKAADAEPVVVAGPVKGEVSVTFQG
                     RGTPEDLARFFLSLSKKAR"
     assembly_gap    27682..27781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27782..29150)
                     /locus_tag="PLP50_06585"
     CDS             complement(<27782..29150)
                     /locus_tag="PLP50_06585"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA51251.1"
                     /translation="MKQKAALPELPLPLRQAFVIVLAATLATGAFAAPAPETDPVVKR
                     AAPGESPRLSIAHDPLKCLSTEARPLVDARVLPGKELDQGFVYFRASGTEDYYYVLMK
                     ARAAEDLVGELPRPLPGIPGIDYYLQALDLASLPKTTPAYAPPVTDKTVCRDERTATA
                     VVTSASPREGLTVGLTRAGQEPVPPGFNKDDIAKVILVTGAVVSLSSALSSGTGAAAG
                     AAAAKAGGLSAGTVFGIGAGVVAAGVAVAASQSSDDENAAPTIGSVSVTPLYGSVPLQ
                     VTATATASDPDNDPLTYSWNFGAGSASGSTASFTFQSAGTFAVSLTVTDGKGGSATNA
                     TVATIRVDAQGTPQYLTGLASWSGDADLDVRLAGPGGVDVATQQGGRRLPAACGAGNR
                     TESVVYEGNRLPFGTYTLYVKHAATCAGGPSTARFSYSVQATSGSKCAGLLDVAPGAE
                     VQACTF"
     gene            complement(29305..31167)
                     /locus_tag="PLP50_06590"
     CDS             complement(29305..31167)
                     /locus_tag="PLP50_06590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA51252.1"
                     /translation="MRKQLGKYLIEAELGRGGMGVVYRAHQPSLERTVAIKLLSSDLI
                     GDPDGVRRFRLEAKSVAKLNHPNIVQVYDIEEEENLIYLVMEFVDGESLDSLISKEVL
                     TESRCLKIVAEIADALHYAHEKGIVHRDVKPANVLMTSEGRVKVADFGLAYLIDREGG
                     TTRTGFLVGSPNYMSPEQAMGQKVDRRSDVYALGVVLFRMLTGRVPFVAESSHAVLFM
                     QVQQEPPDPRDMNKGISLVTRGLVLKALNKKPEARFQTMAELRDAALRQAAALSAGMA
                     GELSLDEGTVALDTSEARASAEKAHVEAHGISMPPVAPERTKATAQIAAPVPPAVAAE
                     PAAPAPPSVVRRGPPRPPARPAAPVRSPILPIAVAVAGLLFLGLGGTLLYKSGLIPGL
                     GRPVPTPPLPLPTAVPTPGLPLPTPTAVFPPATPFPQPTVVPTEAPPLPTPRVEPTKP
                     IVVRVPTPRPTARIVVERPASTPAAGGGLCVQMGPTSFAQGKTSGFAPGFDVGSGPQA
                     RAAKPDLGRFYIEFEVRPSRPQQGQLVTIEAYFSNEAERDVALDHVEENVGMSVTNFR
                     PVTSPPPPAKVRVGRKEPVWAFQGPLGASFLKTIKVTDGTGDSWSRTLEINPCP"
     gene            31186..32430
                     /locus_tag="PLP50_06595"
     CDS             31186..32430
                     /locus_tag="PLP50_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51253.1"
                     /translation="MTECTSAAAASLPPPPPPGEEAWGRAAEAAVEALRLDPQRTRAG
                     LLCVAAGAAVLVALVSIVQGGRRELLRTVEAAGPSNVFVRAERGAPTPLTTAGLSAAR
                     ARFPGLREATGVRIVPAAVHGGTLELPATVYGVGPEALQLFELRRARGRLLGPHDARS
                     RSRTALLGARLAAGLARHGDPVGTRLTAGGETYEVVGVLEPSASVAGSGGELAGVEWD
                     SGLLVPLGAEPDAASAPEGEMPVDLLALRFSDPGEAARATRLLAPLVPTGAAVTTPTQ
                     AIEQYRAAHRSFDRVVLLVSVLTAASAAFGVTNLLRASVRARSLEIGLRRAAGARRED
                     VRLQFLAEGLLLGFGGGLLGILLGVLLSVTLLSRAGWTPHFAPGTLLLLAGGSAAFGI
                     AAGIRPANEAAALDPAATLRLE"
     gene            32427..33833
                     /locus_tag="PLP50_06600"
     CDS             32427..33833
                     /locus_tag="PLP50_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA51254.1"
                     /translation="MIAPLLLAALLAAPPASEPLSVERAVELALTGNPELLDAVDRSA
                     LAAANLSAARSRYLPQVTPYLLPSFRSGDGAAVSRYGVSVSEQLPFGPRFSGYAEASR
                     LEGEEGWDSFHSLTLSQPLLRGLDPAVTREPLRAARRQVAAEERNLGVQKRLTVVAVW
                     SAYLSAALQDELVREAAGRTTRAERILEASRAKEEIGSVSRLDVLRAEQLFAQARSQE
                     NDARNAREDAHDLLARLLGLPAGSRFRLEPPTALPVEVPGLEEALDAAAASREELVEA
                     RERVTDAEAQLRIARSLLLPSLDAGLTWSAAGRSDGFGGAFGNPGGSTWTLGFTSQAP
                     LNLGAQLSARTQAEVSLRIARRAVETLEADVARLVRSAGRRLATSRERLALDEANEEV
                     ARMQLEVAELRFGKGLTDNFFVVDAEGLYNAARVSLLTSRREVLLDGLRLLAEAGLLR
                     PEAFLPRPAPAPYTPSSR"
     gene            33830..35314
                     /locus_tag="PLP50_06605"
     CDS             33830..35314
                     /locus_tag="PLP50_06605"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51255.1"
                     /translation="MIEAESVAPAVRRLREAFSRLAGLPPRTKALLGGGAAVALLGAG
                     LVLRQAAVSDAVGEVRAVRGPLRITVAVAGALAPARAESYGPEIPGVELKVVELVPEG
                     TWVAPGALLVRFDDGPFRRELETARSRLVQTSGEAEQARQALRALLATQRSERLEAET
                     AFARAELDLSTFVNGVAPLQVQQSAAAVERAKREAAESTQKLEGLAPFAEKGYVSRDE
                     LRAAERRATEAAADLVLAEQQHRTLTRFTHPQLLAQKSQEVASRRNALRVVSEKTSAQ
                     AAQAKAAADLAGARAAEAAAAVADVERKIALCTVEARSSGLAVHREIFEKGGEKRRVR
                     VGDAVFAGQPVLDLPDLSEMLVETRVKEGEIHRVAPGQPAEVRLEAFPDEVFAATVLR
                     LGALTAGDRGEARSFPLVLKLAKADPRLRPGMTAQALVLAGEVADAVQVPIDAVRYEG
                     DAAVCTVKTVTGAETRRVKTGRSNAFFVEITEGLAEGDVVLLAR"
     gene            35386..>35594
                     /locus_tag="PLP50_06610"
     CDS             35386..>35594
                     /locus_tag="PLP50_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51256.1"
                     /translation="MDRERWSSYPAATEEERTLLAVLNGTAVRKTLPVVSTRGDERSV
                     EATFAVEAAEPFDGKAEVVVRLVFHG"
BASE COUNT         5032 a        12981 c        12675 g         4706 t
ORIGIN      
        1 cgagcttcac cggcacgccc cgggagcggg cgagctcggc gaccgactcg aaccatcgct
       61 cctcgccggg ggcgtccgga tgggtgtaga cgaaccggac cggctcgccg gcgtcgagga
      121 gccacttcgt gcagcggtac ccgacgtcgc tatagccaca aacgacgatt ccacccatgc
      181 cctaccccct gtcccggccc ggggggtccg gggggtgcaa gacaaacgca ccccccgggt
      241 ggccgacgat tccgcccatg acttaccccc ttctccaatt cgggggggcc gtggggtgca
      301 cggctaacgc gctccccggg tggtccacga ttcccctcat ggctccctcc ttcccccgtg
      361 cacgagccgg acgaggaagc gaggccggcc ccgcacctgc tggtagatcc ggccgacgta
      421 ctcgccgacc atcccgacgc ccgccatgat cacccccatc accatgaagg cgagagcgaa
      481 aagcgtgaag accccctcga cctccgaccc gacgacgagc cggcggacga ggaggtagat
      541 gccgaagccg accccgaaga gggccgtcac gaacccgagc gggccgaaga gctggagagg
      601 gacgagggag aaccccgtca tcaggtcgaa gttcagccgc acgagccgga ggagcgagta
      661 cttcgactcc ccctccgccc gctcctcgtg cgccacgtcg atctccacgg ggcggcgcgc
      721 gtagagctgg gccagcgccg ggatgaacgt cgagacctcg tccgaggccg tcatcacgtc
      781 gacgacgtcc cggtggtacc cgcggagcat gcagccgtag tcgttcagcc gcattccggt
      841 caggaggccg gtcgtccggt tcacgatgcg ggacgcggcc ctccggaaga ggctgtccct
      901 cctcttcgag cggatcgtcc cgacgacgtc gaaaccctcg tccagcttcc cgacgagctt
      961 cgggatctcc tcgggcgggt tctgcaggtc cgcgtcgagg gtgatcacgt actccccgtc
     1021 cgtctcgccg aaggcggcca tgatcgccat gtgctgcccg gcgttccgga ggagctcgac
     1081 ggccttcacc cggccgggcc gctcggcgac ggcggccttc aggagctcga gcgagcggtc
     1141 gcgggagccg tcgtcgacga agacgacctc ccagggccgt ccgagaccct cgaggacggg
     1201 aacgagtcgg gggaggagcc gcgggaggtt cgcctcctcg ttgtagacgg ggacgacgac
     1261 ggagaccttt cgcggcacgg tcggagggct cgtcacggga tgacgattct aggggacgag
     1321 ccgagacgca tttgaacggg tcccgaaccg gcccgggaca ccgccctcgc cctcaggggc
     1381 ggttcgagac gaggaggaac ttcccgttcg ggctccgggc caggacgtgc ggcagcgaaa
     1441 ggcccgcggc cgggttcccg aagtcgcgca ggtggccgaa gtggacgatg cagaggaccc
     1501 tcgccgggcc gtgccacttc ctcgcgaact cctcgtcgga gagatagagg ccgcgccgcc
     1561 gcgcctcctc cttcccgaag tcgagctcgc tccaggggag gtctgcgacc ggcgtgagcc
     1621 gcttcgtgta gaaggccggc ccctggaggt agtgcccccg ctgaaggagg aggtcgtcgg
     1681 gtcgcagctc gcgcttcagc gtggcgacga gcggccacgg cgtgatcgag cgggcgaccg
     1741 agggaagcgc caggacgagc gccccggaga agccgagcca cggcaggacc attccgaggg
     1801 cgaaccgttc cgtcgagaac gcgcggcggg gcgcacgggc ggcagaccgg cccgcgagga
     1861 ggttcaggag cgcgcccagg gccagcgacc cgaccgcgac ggcggcctgg acctccacgc
     1921 cgtaccgggc cgcgagaccg gccccgaacg cgtacgcggc cgtggcgccc gccagcaggc
     1981 cgaacagcag ccccgcggcc cggcgctcgc cccgcagcgc cgccccgcgc tgtcgcgccc
     2041 gctcgaggcc cagggcgagg aggaccgcga cggcgggcca gatcggcagg agatacggca
     2101 cgagcttgga gcgcgaggcc gagtagaaga cgaggacgag gatccagaag acctgcaggt
     2161 atgcctcggc gccccgttcc cgccaatcgc cgcggcgaag cgaggggaac gcccccgcga
     2221 ggcgccccag gaagggcgtc cacgggacga gccctcccag gaggacgagg acgaagaaga
     2281 acggcttcgc cgtcctccgg tgccccgtct cgaagtacct tcggaagtgc tcgcccacga
     2341 cgtagaagtc gaaccattcg ccgtctctcc gggccatcgc cacgtgccag ggcacgacga
     2401 ggaccaggaa gaggaggagc ggccccgggg agaagagctt cggaacgatg ccgaggcgtc
     2461 gtgagaggag ggcccagacg aggatcgcgc cccccgggag gacgagcccg acgaacccct
     2521 tcagcatgac ggccgccgcg caggcgaagt ggaggaggta gagcgcgatg cgcgcgcgcg
     2581 cgcgatcccc cgatcgctcg tgctcctgga aggcggcaaa ggcgaacgcc gccgaggaga
     2641 gcgcgaacga gagcgggccg tcgatgatgt tcaggcgggc gagggcgacg acgaggccgg
     2701 aggtcgcgac gacgagcccc gcgacgaggg ccacgcgctc gccgtaccgg cgcctcgcga
     2761 agaggaccgt cacgaggacc atcccggccg ccgagagctt caccggcagg cgggcggacg
     2821 actccgactc tccgaagagg gacatcgagg ccgcgaccgc ccagtagtgg aggggcggct
     2881 tctcgtagta acggaagccg ttcaggcggg gcgcgacgag gtcgcccgtg gcgagcatct
     2941 cgcgcgggat ctccgcgtac cgcgcctcgt ccggctcgaa gaacggcgtg tgccccgtgt
     3001 cggcgacgag gacggccacg gcgaggatca ggagcgtcca gagcagcgcc cgcgaggccg
     3061 gcggcagggg ctcggaccgg aagcggctcc cgctggtcag gtacgcgccc acgtcgcccc
     3121 tccttcgacg gtccagctcc gctccggggc ccacacgccc gcctcggtcc gcggcgagtc
     3181 gacccggaag cggcaagccc cgcgccagta gtcgtaggga gtcccgttcc gcgcgtggat
     3241 cgcgacgtcg acgaggtagg tccccggacc caggtcgcag cgcgggatcg tgacccgcac
     3301 cttcgcggcg gcgtcgaacc ggccggccgc gaagccgtcg atctccgtgt tcgagccgtg
     3361 gacgcagacg tcctccggcg tgaagagccc cacgccgaag acgaaatcgt cgagcgggcg
     3421 cgccggcgcg gcctcgacct cgatcgtgac cggctcaccg gtccggaacg cgtaacgctc
     3481 gaggccgtcg cggtcgagga gcctcacgcc ttcgatcgtc gcggagcgat cgccccagcg
     3541 ccgcgccctc tctgcggggg ccggcccggc ctccaagccg tccccttccg ccgcgccggc
     3601 cggctcgggg acgggagccg cggcctcggc gcggagcgcc tcctgcccca cggcgtgctc
     3661 ggcgaggagg cgtgtccctt ctccggccgc gacgtcgagc cggtaggccg cgagcatctc
     3721 ccgcggctca ccgaggccgg cgacccggcc tcccgacaga tagaggacgc ggtcgcacag
     3781 ctcctcgacg agggcgaggg agtgcgagac gaagaagatc gtcctgccgc gcgtcgagaa
     3841 ctccttgatc gtgtcgaggc agcgccgcga gaaggcctcg tcgcccacgg cgaggacctc
     3901 gtcgacgacg aggacctccg ggtcgacgtg gacggcgacc gcgaagccga gcctcacgta
     3961 catcccggac gagtaggtct tgaccggctc gtcgatgaac tccgagagcc ccgagaactc
     4021 gacgatcgcg gggagcttcc tctcgatctc cttccgcgtc agcccgagca tgacgccgtt
     4081 gatgacgacg ttctcccgcc ccgagatctc cgggtggaag ccggcgccga gctcgatgag
     4141 ggccgagacc ttcccgtcga cgcggagcgt cccggaggtc ggcttgaaga ggccggcgac
     4201 gagcttgagg agcgtcgact tcccggagcc gttcgccccg atgacaccga acgtctcccc
     4261 gcggcggacg tcgaacgaga cgccggagag cgcggagacc gtctccgagg gctccagcgc
     4321 ccggccgagg ccgcggccga ggagcgccga cttcagcgtc ccgaacgcgc gcgtccggcc
     4381 ccagcggcgg tagaccttcg aaaggtcgcg cgcctcgacc gcggagcggg cgcgcgggct
     4441 cagaccgcct ccgggaacga gtcgcgcagc cggtcgaaga agacgaatcc gacggcgaag
     4501 gccgcgacgg agatcgccgc cgccgtggcc cacgtctggg gcgggctgac gcgcccgtag
     4561 aagagggcgt cgtgccagcc ggcgaaaagc ggcgcgaacg ggttcagcgc gaggagacgg
     4621 gtcagcttcg tcttcgggag gtcgttcagg gtgtagagga tcggtgtcgc gaagaagagc
     4681 accgagagga ccgtctggag gaggtccttc acgtcgcgga agtggaccgt gagcgcggcg
     4741 aggaagagtc cgatcccaag caggaggagc ccctgcagga cgaggagcgg gatcacctgg
     4801 aggagcggcc agccgagagg gaccttcgcg ccgaacgcgc cgagggcccc ggcgacgagg
     4861 gccgccagga ggaccgggag cgcgaggagg aagtgcacgc cctgcgcgag gaccgccacc
     4921 gccggaagca cttcggccgg gaagaggatc ttgcgcagga gcgcgccgtg cgtcaggagc
     4981 gaagaggccg cgtcggtgat cgccgaggag agccagttcc agggcagcag gccgcagaag
     5041 aggaagaggg cgtaggggga ggtcgaaggg ctgcggttcg ggaggaggag ctggaagacg
     5101 agggtgtaga ccgcgagcat caggagcggg ttcaggagag accagaagaa accgagaacg
     5161 ctcccgcgat agcgagcctt gacctcgcgc gtcacgagga cgccgacgag cgcccggtgg
     5221 cgccagaggt ctcggagccg ctccattccg accgctgatg atacgcggcg ccgcgcttgc
     5281 cgccccatcc ccgcgccccg gttcgccgta gtgtcgtcgc atgggagcgc gcgtcgaggt
     5341 cgtcgaggtc cggaagtcgt ggggagccgg cgggagcggc ggagacgcag ccagcgcgga
     5401 agcgctcgcg ggggtctcct tcgtcctcga ccccggggag ttcgtcgcgc tcctcggacc
     5461 ttcggggtgc ggcaagtcga cgctcctcca cctcctcggg ggcctcgacc gcccgagctc
     5521 gggtcgggtg atcgtggacg ggagcgacct cgcgcgccta tccgaggcgg accgcgacct
     5581 ctaccgccgt cggacggccg ccaccgtctt ccagctcttc aacctcctgc cgacgatgac
     5641 ggctctcgag aacgtcgccc tcccgctcct gctcgacggg gtcccggcct ccgaagccga
     5701 cggacgcgcc gagaggctcc tcggtgaggt cgggctggcg gccaagtcgg gctcctttcc
     5761 gtggcagctc tcgggcggcc agatgcagcg cgtcgccgtc gcgcgcgccc tcgtcgcctc
     5821 gccggcgctc ctcctcgcgg acgagccgac ggggagcctc gactcgcgaa gcggcgcgca
     5881 ggtcctcgac ctgatcgcgg agctcgcgtc ggcccggagg gtgacggtcc tcctcgcgac
     5941 gcactcggac gaggccgcgc ggcgggcgag ccggaccctg cgcctccgcg acggccggct
     6001 cgagcggtga gccggacccg gccgctcctc gccccgctcc ttctccgccc gttccgggag
     6061 gaccgcgggc gcttcgtcct gaccgtcgcc ggaatcgccg tcggcgtcgc gacgatggcc
     6121 gcgatcctcc tcgcgaacgc atcggtcctc tcgtccttca cggcgacggt cgacgtcgtg
     6181 gccgggaagg cggggctcac cgtcctcgcc gacgggccgg gaatcccgga ggagacgctc
     6241 gagcggctcg cgtggctccg catgaagggg gtcgcgatcg tccccgcggt ctcggtgacg
     6301 gtcgccctcg ccggaggcga gggcgacgtc ctcgagcttc tcgggatcga cccggtcacg
     6361 gacccttccg tccgggacta ccgcttcgtc gcggagacgg cagacgagac gtcgctcgag
     6421 aagctgttcg ggatcttccg ggccggagcg gtcctcgtcc ccgcgccgct cgccgaacgc
     6481 ctcggcctcg ggccgggcgc ggagctcggg gtcctctcgg ggggcgccgc gcaccggctg
     6541 cgcgtcgccg gcgtccttcg gatggaaggg gcggcgcggg cggcggccgg ctcggtcctc
     6601 ttcgccgatc tcgcgacggc gcaggaggtc ctcgggcgcg agggcttcct cgaccggatc
     6661 gacctcgtcc ttccggccgg cctcgccgac gccgaccgga cggccctcga ggcctccgtc
     6721 cgcgattcgc tcccgcccgg cgtcaccgtc gggcgccccg agcggcgaac ggagacggtc
     6781 gaccggatgg tccgttcctt ccgggtcaac ctgacggccc tcggcctcgt cgccctcctc
     6841 gtcgggacct acttcgtcta caacacgctc tcgatctccg tcctgcggcg gcggagcgac
     6901 atcggcaccg tccgctctct cggcgcctcg gcccgctcgg tcttcctggt cttcctcgcg
     6961 gagggggccg cgctcggcgc cctcggaggg gcgctcggcg tcgcgctcgg ggcgctcctg
     7021 gcgcgagtcg ccctcgccgc cgtcggcggc accgcgaccg acttctacct cccgcaggcc
     7081 gcgccggtct tcacgctcgc cccgtccgcg ctcgcgacct cgttcctcgc cggcctcggc
     7141 acgtcgatcc tctccgccct cgcgccggcc gtcgaggccg cccaggtgga gcccgcatcg
     7201 acgctccggc acggctcggt cgaggcttcg cgccggcgtg ccgtggggcc gctcgccctc
     7261 gggggcgtcg ccgccctcgc gctcgccgcc ctcgcgacgc gtcccggacc ggtggacggc
     7321 ctccccctct tcggattcgc gtcggtcttc ctcgtcatcg cgggcgtctc cctcctcgcg
     7381 ccgctctccg tcgtgaccgt ggcgcgcctc gcggacggtc cggccgtccg gctcctcggc
     7441 gtcgaggcgc ggatcgcccg cgcgaacctg accgggagcc tctcgaggac ggccgttgcc
     7501 gtggcggccc tttcgatggg gatctcgatg atggtcgcgg tggcgttcat ggtcggctcg
     7561 tttcgcgcga ccgtcgtcga ctgggtctcg cagacgctga aggccgacct cttcgtcgct
     7621 cccgagagcg gccgttcgca gcgcgcgctc gggcgcctcc ccgaggaagc gatccgcctc
     7681 gtcgaggcgg tgcccggcgt cgcctccgtc gacccgttcc tctcgttcga ggcgacgcga
     7741 gacggagctc cctacacgat cggctcgggc cgcttcgacc tcgtcgccgc ccacggcgac
     7801 ctgccgatgg ccgacgggag cgacacgcgc aaggtcctcg cacgcgccct cgcgcggggc
     7861 gaggccgtgg tctccgagcc ctactcggtc aagttcggcg tgaacgcggg agacacggtc
     7921 accctccccg ccctcgacgg cccggtggcc cttcgcgtgg caggcgtcta tcgcgactac
     7981 tcgaacgacc gcggcaccgt gacgatcgac cgcgcgctct tccgaaggct cttccggatg
     8041 gacggcgccg ggagcctcgc cgtccgcctc gagcccggag tcgcgccgga ggaggcgcgg
     8101 cggagacttc tcagcgcggc ggacggacgc ttcgcgctcc gcgtccagac gaacgccgcg
     8161 ctccgggagg cggcgctggc cctcttcgac cggacgttcg ccgtgacctg ggcgctcgag
     8221 gccgtcgcga tcgccgtcgc cgtcctcggc gtcttctcga cgctcctcgc gctcgtcctc
     8281 gagaggcggc gggagatcgc gctcctgcgc gtcctcgggg cctcggccgc tcgcgtcgcc
     8341 cgcgccgtcc ggctcgaggc ggccgctctc tcctcgctcg gtgtcgtcct cggcgtcgcc
     8401 gcgggcgccg cgatgggcat cgtcctcgtc cacgtcgtca accgccagtc gttcgggtgg
     8461 acgatccgca ccgacgtgcc gtggctcttc ctcgccggcg cgctcgcgct cgtcctcctc
     8521 gccacgctgg ccgccgcttc gcgccccgcc cgcctcgccg cggcaaccga ccccgccgcc
     8581 gtcctgaagg aggagtagtc cgatgtctcc gtgtcgcgcc ccggtccgcg tccggcccgt
     8641 tcgcgagctc gtcgcgtctc ttcggccggc cgccgccctc gctgcgcttt cgctcctctt
     8701 ctccttcgcc ccgctcgctg gcgagggcgg ccgcttccgc cctctcgacc cgaaggcgac
     8761 gcccgccttc ccgcgagacc acggcgcgca cgacgacgcc cgcgtggagt ggtggtacgt
     8821 caccggccac ctcgtcgacc gcgacgggcg ccggtcgggt tttcagctga ccttcttccg
     8881 gaccggcctc gtcgacgagc cgaagggggc tcgatcgtcg gccttcgccc cgcgcgacct
     8941 ccacctcgcc cacttcgcgc ggaccgacgt cgacggaagg gcgttccgct acgccgagcg
     9001 gacccaccgc gacgggccgg gaggcgcctc ggcccggacc acgcacctcg acgtcgcgaa
     9061 cgaggactgg aggctcaccg agctcggcgg gaagctctac ctgcgggcga gcgaccgggt
     9121 cgacggcgtc gaggagactc tcacgctcct cctcacgccc gagaagccgc ccgtcctcca
     9181 cggcgagaac ggcctctcga agaaggccgc cgagcccgac gccgtcagcc gctacgtcag
     9241 cttcacgcga ttgcgggcgg aggggtgggc gacgcgcgga gacgaggccc gctccgtgac
     9301 cgggagcgcc tggatggacc acgagtgggg ctcgggggcc atcggccgtg ggacccgggg
     9361 ttgggactgg ttttccgtgc agctcgaaga cgggcgcgac ctgatgatct accagctccg
     9421 gggcgaagac gacacgcgga ctctccactc gtcgggcacc ctggtcgaga gggacggccg
     9481 cgcgacgccg ctggctcctg gcgacttttc cgtcgaggtg acggcgcgct ggacgagcca
     9541 gcgctcgaag gggacgtatc ccgcgcggtg gctcctgcgc gtcccgaaag cgagcctcgc
     9601 gctcgaggtc gtccccgttc tccccgacca ggagctcgtc accgaccgct ccacccgcgt
     9661 cacctactgg gaaggggcct gcgacgtccg cgctcccggc gccggccgcc cgctcggccg
     9721 cgcctacgtc gagctgaccg gctatgccgg caagggcgcc ctcggcctct tctgacgcgc
     9781 cgtgatggac cgtgtggacc gtggggccgt gtctgcactc gtgtattgtg tggcaaacaa
     9841 tacacgagtg cagacacggc cctcagcgct tagccgcttt cgcttcggcc ccttccccgg
     9901 tgaacccgtg agggcgccgg ggctcagaag ccgaggttct tcgcgatgtc gaaggcgagg
     9961 accgagacca tcaggaggac gaggaagacg aatcccacct ggaggagccg ctccttgaac
    10021 ttcagggaga agtcgcggcg gaagatccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnncc cacctggagg agccgctcct tgaacttcag ggagaagtcg cggcggaaga
    10201 tcccctcgaa ggtgaggacc atcagctgtc cgccgtcgag gagcgggatc gggaggaggt
    10261 tcaggaggcc gagctggagc gagaggaggc ccatgaagcc gaggagcgcc gcccatccgg
    10321 tccggaccgc ctcgcccgag aacttcgcga tgtcgaccgg gccggagagc tggcggatcg
    10381 agcccgagcc cgagaccatc cgcttgatcg tctgcccgac cgcggacgtc tgcctcaggt
    10441 tctccttcca ggaggcgccg atcgccgcca ggaacggcat cttccgctcg acgagctcgt
    10501 acttcggcgt gaagccgatc ctccacgagc cgccttcgaa cctcggcgtg acgtccagcg
    10561 cgacgcgcgt cccgttccgg tcgacctcga aggagatcgg ctgcggcttc ccttcctcga
    10621 acgcagagga cgcctcccgg acgcgccgca cgacctcgaa gtagccgtcg atggcgacgc
    10681 ccccgatcga gacgatccgg tcgccgacct ggagccccgc cttctctccg gggtagccct
    10741 tcacgaggct cccgatgacc ggcgcgagcc aggggttcag cccggtgtag ccgatgtcgt
    10801 acttcgtctg cggctcgggt cggatgacga cggagacgga ctccccgccc cgctcgagga
    10861 cgacggggat ctccgcccgc ggcgccaggc cgaggtgtgt ctccacctcc cgccacgtcg
    10921 agaccggctt gccggagagc gagacgacga cgtcgtcgat cctcacgccc gccttctcgg
    10981 ccgggctgcc ggcgtcgacg tgcttcacga ccggtctctc ctcggcgtac ttcggcaccg
    11041 tcacgccgat cgtgaacgcc cccgccgtca ggaggagggc gaggacgagg ttcaccgcgg
    11101 gtcccgcgag gaggacgagg aagcgctgcc agcgggtgcc gcggagcccc tggtccgctc
    11161 cggcgtccac gacgtcgctc tcgtcggggc cgagtccgat gatcttcacg tacccgccga
    11221 gcggaacgag ggagacccgg tagtccgtcc cgttcctctt gaacccgaag aggcgcttgc
    11281 cgaagccgat cgagaagacc tcgaccggga acttgaagag ccgtgccacg aggaagtgcc
    11341 cgaactcgtg gaagaagatg aggaacgaga ggacgaagac gaaggcgagg atgttcgtgg
    11401 agatctgcat cggacccttt gcttacgccg aagccggcgc gacggttctc ggggaagcaa
    11461 gcggcgcgcc ggaggagacc agctcggccg cccggcgtcg ggcctcccgg tcggcctcga
    11521 ggacggcctc gagcgtcgtg gccggggaga cgggatggtc ggcgaggacc cgctcgacga
    11581 cggggacgag cgaccggtag gggatcctcc cctcgaggaa tgccgcgacg gcgacctcgt
    11641 tggcggcgtt caggacggcg ggagccgttc cgcccgcccg gagcgccgcg taggcgagcg
    11701 ggacggccgg gtagcgcctc tcgtcgaggg ccgcgagctc gagcgtcccg agagacggca
    11761 ggtccagctt cggcatcggc gtcgggaccc gctcggggta cgtgagcgcg tagaggatcg
    11821 ggaaacgcat gtcgttcggc gagatctgcg cgatgaccga gccgtcgatc cactcgacga
    11881 aggagtggac gatcgactgg gggtggatga cgacgccgat ccgctcgggc ggcacgtcga
    11941 agagccaccg cgcctcgatc acctcgagcc ccttgttcat catggtggcc gagtcgatcg
    12001 tgatcttcgg ccccatcttc caggtcgggt gggcgagggc gtcctcgatc gtgatcgcgt
    12061 cgaacgtctc gagcggccgc ttgcggaacg gaccgcccga cgcggtcagg acgagtcggt
    12121 cgacctcctc cggccggcca cagcggagcg cctggtgaag ggcgcagtgc tccgagtcga
    12181 ccggcaggac cgcgcttccc gaggcggctg cggcggccat caccgcctcc ccggcgacga
    12241 cgaggacctc cttgttcgcg agggccagag tcttgccgag cttcaccgcc tcgtacgccg
    12301 gagggagacc ggccgctccg acgaccgccc cgaggacgac ctcggcctcg gggtgcgtcg
    12361 cgacgtcgat cattccctgc tcgccgaagc cgacgcggac ccccgggaac tcgcgacgga
    12421 cccggtcggc cgccgcccgt tccttcacgg agacgacggt cggccggaac cgggcgatcg
    12481 ccgggagaag cgcgtcgagg ttcgagccgg ccgacatcga gacgacccgg aaccgctccg
    12541 gcagcgcctc gaagaccgag agcgccgagc ggccgatcga gccggtggca ccgaggaggg
    12601 ccacgcctcg cggagcgttc atcggggtcg cagctccggg aggagggcga ggatcccgaa
    12661 gaggacgggt gaggcgaaga ggagcgagtc gagccggtcg agaaggccgc cgtgccccgg
    12721 gaggaggcct cccgagtcct tcaccgagca gctcctcttg aacgtcgatt cgacgaggtc
    12781 gccgaccacc gcgacgacgg acaggacggc gccgaggagc gctccgcccg cgacgaccgc
    12841 cgggggctcg ccggggatgc gcgggaggac gagcgcgagg agggctccga acagggcccc
    12901 ggcgatcacc tgtccccaga agccctccca gctcttcttc gggctgacga ccggggccag
    12961 gcggcgcttt ccgaaccggg tgccggcgta gtaggccgcg gagtccccgg cccagacgat
    13021 cccgagaagg aagacgaccg gctcccagcc gatcccgcga agcgcgacga tcgccgcggc
    13081 tccggcgccg acgtagaggg tcgcgaacga ggtcgtggcg ctggaggcga gccagaccgg
    13141 ctctgccggc gcggcgagca ggacggccga cggcacggcc acgaggacgg ctccggccgc
    13201 cgccacgagg agcggcgccg agccgaggta tgccgcgacg acgatcgtgg cgaagaggac
    13261 gaagctgagg gcccgcgggg cgagccagcc gccgcagcgg atgaggccgt ggaactcgtg
    13321 gagggccgcc gcgcaggcga ggccggcgag aacggcccac gaaagaagcg gccccttcac
    13381 gaggacgagc aggacgagcg ggatgaggac cgccgccgtc agctcccggg tgcgcctcac
    13441 gcgcccaggg ccgccggcgc gggcggggcg ccgggggcgg aagcgtcctc ctccgtgacg
    13501 ccgccataac gcctctcccg gcgctggtag tcgaggaggg cttcgagaag atgccgacgg
    13561 cggaagtcgg gccagaggac cggcgtgacg tggatctccg cgtaggcgat ctgccagagg
    13621 aggaagttcg agatccggag ctcgccgctc gtccggatga ggaggtcggg atcggggagg
    13681 tccgccgtat agagacggcg ggcgacggcc gcctcgtcga tcgactccgg gtcgaggcgt
    13741 ccctccgcgg cgtcgcgggc gagagcccgg gccgcgtcgg cgatctcggc tcggcccgag
    13801 tagttcagcg caacgctgaa gaccatgccg cgacaggagg ccgtggcttc gagcgccgtc
    13861 gagagcgccg accggaccga ggggtcgagg ccgtctggcc ttccgacgac gcggaagcgg
    13921 atgtcgttct cgacgagcga ccggaactcc cgccgcaggt actccttcag aagcgcgaag
    13981 agcgtcgaga cctcggcctt cggacgcttc cagttctcgg tcgagaaggc gtagagggtg
    14041 agcgcgtcga ggcgcaggcg cgcggcggtc tcgaccgtct cgcgcacggc ggacgcgccg
    14101 gcccgatgcc cctcgacacg gggcaggtgc cgctgccggg cccacctgcc gttgccgtcc
    14161 atgatgacgg cgacgtggcg cgggaggcgt ccgaggtcca gcgtgtccag cagagcgcgc
    14221 tcgggggtgc ccggcgcggc gacgaggtcc ttctcgatca tctgtccgac agccggaccg
    14281 ggatcgcccg ggaccggctg aatttctgat cttaccctgc ccggtagaag accttctcga
    14341 gcgtcagacc gcgggccgga gccgtcgggc cggggcgccg gaacgcctcc cccgggtcgg
    14401 tccgcccgag gccggcttcc acgagcgtcc caacgaggcg gcgcaccatc cctcgcagga
    14461 acccgtcccc gacggcggtg acgacgacga gcggaccgag ctctccgacg tcgagccgag
    14521 cgagcgtccg gaccgtcgtc cggcggggcg ccccggcgag accgaacgac gcgaagtcgt
    14581 tccgaccgac gaggcgggcc gcggcagctc tcatggcgcg aacgtcgagt cgcggcgagg
    14641 ccaacgcctc gcgcagcccc tcgaacgggg ggaggtgatc ggcccggcgg agccggtaga
    14701 cgtagcgctt gccgagggcg tcgaagcggg catgccacgc gaacgggacc tcggccgcgt
    14761 cttcgacccg gacgtcccac gggaggaggg cgttcaggcc gcggcgcagg ccttcgcgcg
    14821 ggacgagagg gccaccggga gggacgtcga aatgggcgac ctgtccctcc gcgtggacgc
    14881 cggcgtccgt gcggctcgcg gcgtgaacgc gcacgcggga gccgtgcatc ttcgagagcg
    14941 cctcctcgac gacgagctgg accgtccggg gaggtcccgt ccccgtccgg acgacgtcct
    15001 gggcctgcca cccctcgaaa tgggtgccaa gataggagag ctcgaggcgg cgcctcgcgc
    15061 cctcggcgcc ggccttgtgg ctgacctcgc cccgaacgct cacgacccga tgatagggtc
    15121 ctccggcccg tccgccggtc tgaccccgga ccttccggag cttgccggcg cccgcggatg
    15181 gccgggcgcc ggcgccggtg cgtccgctcg ggcggagacg gtccggtcag gcgcgaaaag
    15241 aaaaggcttc cggggcgggg ggaaggacga cgttcacgat ctcgagccgc tcttcggtcc
    15301 cgccctcacc gtacggaagg ggatccccga cgcgcacgat cgcgagcccg tcccccttcg
    15361 tcaggatcgc gagagggcgc cccggatagg agcgccgcag ggagacgagg gcgtcgtctc
    15421 cgtctcgagc gaggaagtag agcgtgccgt tccggtttcg gaccaggatc atgcgactgg
    15481 gaaggtagct cgcgcacccg ccgcaccgca tcacccgaac gggtgaatca gctgcggagc
    15541 gcagaaatct tcatctgtgc tgtagataga ttcttgacaa taacacacgc agatactaca
    15601 ttctcggacc ggaggacgtt ccgatgaacc ccgaccgcct cacactgaag gtccaggagc
    15661 ttctccaggt aatcgcccgg gacgcccgat ccgccgggaa tcccgaggtg acgccagagc
    15721 acctcgccgc ggcgctcgcg gcggagccct cggtcgggac ccctctcctc acccggatcg
    15781 gggctccgcc ggcgctcctc gccggcgagc tcggtgccct cgtcgcccgg ctgccgaagg
    15841 tccaggggtc cggggccgag acccgcttct ccccggccct cgtcaggatc ctcgaccgcg
    15901 ccgaggcgac ggcgcgcgag ttccgcgacg agtacgtcgc cctcgagcac ctcctcctcg
    15961 cgctcctccg cgacggggcg tcgaaggcgg ccgagatcct gaggcggcac ggtgtcaccg
    16021 aggcggccgt cctttccgcc ctgaaggagg ttcgcgggag cgcgcgtgtc gacgacccga
    16081 acgccgagga gaagtaccag gcgctgaagc gctacgcccg cgacctgacc gagctcgccc
    16141 ggcgcggcaa gctcgacccc gtcatcggac gcgacgacga gatccgccgc gtcatgcagg
    16201 tcctgacgcg ccggacgaag aacaacccgg tcctcatcgg cgacccgggc gtcggcaaga
    16261 cggccgtcgt cgaggggctc gcgcgccgcg tcgcctcggg cgacgtgccg gagagcctga
    16321 aggacaagca ggtcgtcgcg ctcgacctcg gcgcgatggt cgcgggggcg aagtttcgcg
    16381 gcgagttcga ggagcgcctg aaggccgtca tcaaggaggt cgaggagtcg gaggggaagg
    16441 tcatcctctt catcgacgag ctccacacgc tcgtcggcgc cggcgccgcc gaaggggcga
    16501 tggacgccgg gaacatgctc aagccggccc tcgcccgcgg cgagctgcgc gccatcggcg
    16561 cgacgacgct cggcgagtac cgcaagcacg tcgagaagga ccccgccctc gagaggcgct
    16621 tccagcccgt cgtcgtcggc gagccctcgg tcgaggacac gatctcgatc ctccgcggcc
    16681 tgcaggagcg ctacgaggtc caccacggcg tgaagatcac cgacgcggcg atcgtcgcgg
    16741 cggccgtcct ctcgaaccgc tacatcgccg accgcttcct ccccgacaag gcgatcgacc
    16801 tgatcgacga ggcggcctcg cgcctgaaga tggagatcga ctcgctcccg accgagatcg
    16861 acgaggtgga gcggaagatc ctccaggccg agatcgagag gaaggcgctc gagaaggacg
    16921 actccgcgcc ggcgcgcgag cgcctttcgg cgctcgagaa ggcgctcgcc gagctgcgcg
    16981 agaggtcgct ctcgatgaag gcggtctgga agaacgagaa ggacgccatc ggcgcgatcc
    17041 gcgacctcaa gaagaagact gaggacgcga aggccgacgc cgagcgcgcc gagcggcagg
    17101 gggacctgag ccgcgcggcc gagctgcgct acggcgtcct gaaccagctc gaccgcgacc
    17161 tcgccgccgc gaccgcgaag ctcgaggagc tgcaggagga cggcggtttc ctccgcgaga
    17221 aggtcgagga ggaggacgtc gcggccgtcg tctcgcgctg gaccggaatt cccgtctcga
    17281 agatggtcga gggggagacg cagaagctcc tcgcgatgga ggagcggctc cggctccggg
    17341 tcgtcgggca ggacgaggcg gtcaccgccg tgtcggccgc cgtccgccgc gctcgcgccg
    17401 ggatgaacga cccgaagaag ccgatcggct ccttcctctt cctcgggccg acgggcgtcg
    17461 ggaagaccga gctcgcccgc gccctcgccg agttcctctt cgacgacgag ggcgcgatgg
    17521 tccggctcga catgtcggag taccaggaga agcacaccgt ctcgaggatg atcggcgccc
    17581 ctcccggcta catcgggcac gacgaggggg gccagctgac cgaggcggtc cgccgcaggc
    17641 cctactcggt cgtcctcttc gacgagatcg agaaggccca ccccgacgtc tggtcgacgc
    17701 tcctgcaggt cctcgacgac ggccgtctga ccgacggcaa ggggcgggtc gtcgacttcc
    17761 ggaacgtcgt cgtcgtcatg acctcgaacg tcgggtcgca gctcatccag gagctccagg
    17821 gggtcgacga ggaggagatg cggaagggga tcgaggggga gcttcggcac cgattccggc
    17881 ccgagttcct gaaccggatc gacgacgtca tcgtcttccg ccgcctcacg ctcgaggaga
    17941 tcgggaagat cgtcgacctc cagctcgcgc gcgtcgccgc gctcgtcgcc ccgcgcggcg
    18001 tgaggctgga gtggaccgac gccgcccgcc gtctcctcgc cgagcgggcc ttcgacccgc
    18061 tctacggcgc ccgcccgctg aagcggaaca tccagaagct cgtccaggac ccgctcgcgc
    18121 tccgcatcct cgcgggcggc gtcccgtccg gctccaccgt gacgctcgac gccggagccc
    18181 cgggcgaggg cgtcgccttc tccatcggga gcccggccgc gcccgcggcc tgacgcagca
    18241 gagcaggagg gagaccatgg ccgccgttcg ccgctacgac cctttccggg agctcgcgac
    18301 gctccaggag cggatgaacc ggatcttcga cgacaccttc tcgagcgcct cgcgccgcga
    18361 ggacgagggg ctctcggcga cgtggacgcc gccggtcgac gtcctcgaga agagggaccg
    18421 gatcgtcctg accgccgagc tccccggctt cgcggaagac caggtccagc tccgcttcga
    18481 ggacggggtc ctgacgctcg agggggagcg gcgcttcgag aaggaggtcg acgaggcccg
    18541 ctaccactgc gtggagcgga gctacggccg cttctcgcgc tccttccggc ttccggcgaa
    18601 cgtggacgag gagtcgatca cggcgacctt cgtgaacggc ctcctcgtcg tcgagctgcc
    18661 gaagcgggag gaggcccggc cgaagagcat ccggatccgc gccggcagcc cgccggcgat
    18721 cgacgtgaag tgaggccgcc ggctcaggcc gcgggccccg cctcgtcctc cttgaagagg
    18781 tccgtcgaga tgtagcgctc ggaggtcgac ggcacgacga agacgaccag cttccccgcg
    18841 ctgtccggac gccgcgcaat ctcgagcgcg gcgaaggcgg ccgcgccgga ggagatcccg
    18901 cccaggatcc cctcttcgcg cgcgaggcgc cgcgcgtact cgaaggcctg gtcgttcgtc
    18961 acctgcacga cctcgtcgac gagcgcggtg tcgaggttcg ccgggacgaa gccggctccg
    19021 atcccctgta tcctgtgcgg cccgggcgcg ccgcccgaga ggaccggcga gtggaccggc
    19081 tcgaccgcga cgaggcggag ggacggcttc ttcggcttcc agtaccggcc gacgcccgtc
    19141 atcgtcccgc cggtgccgac gccggcgacg aagacgtcca tcgccccgtc cgtgtcgttc
    19201 cagagctcga ccgcggtcgt cgcctcgtgg atcgcgggat tcgccgcgtt ctcgaactgc
    19261 tgcggcatca cgccgttctc gccgagctcg gcaaggcgct cgttcgcgcg ggcgatcgcc
    19321 cccttcatcc cctccgcggc gggcgtcagg atcagctccg cgccgaggag ccggagaacc
    19381 ttccggcgct cgagggagac cgactcgggc atcgcgacga ccagccgata ccccttcgcg
    19441 gcggccacga aggcgagcgc gatccccgtg ttccccgacg tcggctcgac gagcgtcgtc
    19501 ttcccggggg cgatcctccc ttccttctcg agcgcctcga tcatcgcgac gccgatccgg
    19561 tccttcaccg agttggccgg gttgaagtac tccagcttcg cggcgacgac cgcgccgggc
    19621 gcggcgtcct tcgccatccg gttcagccgg acgagagggg tgttgccgat gagcttcgtg
    19681 acgtcggacg cgatccgcat ggcggcctcc tggactccgg gttccccggc gtcaaatctc
    19741 ccagtcgatg acgtggcgat cggcgcggtg ccgggcgacg aggtcgccga cggtccaccg
    19801 ctcgtagacg gcgaggcgcg ccgcgacggc ttcggactcg accgcctcga ccagccgtga
    19861 aaggcccttc ccagccgcgg cccggccgcg accggacggg gcgccgaacg gagcaaggac
    19921 ggccgaaagc ggagagccga gaagcccctc cccggcacgg tacccgccgg cacggccccg
    19981 gaccgcgacg aggagccccg cgcggacgta cggcgcgaga agctggtgga ccgattctcg
    20041 cgagagcgcg aggaggcctg ccaattcggc ggcaccccgg tcccgttccg ggtccgaacc
    20101 gatctcgacc atcgcttcga ttgcctttct ctgcctgagg ttaagaatct tgaaatctcc
    20161 gttccacttt actaacatta ttttgaggcc gaatctcaga atgtcaagac acacgcttgc
    20221 cgccgccgct ctccttcttt ccgcctcgac ggccctcgcc cagcggggag gctccgaggt
    20281 ccgccgcgcc gaggacgatc cccggaggag ctcgatcgtg agggcggtcg agcagatcgg
    20341 tccgtccgtc gtgaacatct cggccgagcg cctcgtccag cggcgggcga ccccgttcca
    20401 ggagtacttc ggcgttcccg gcgaccgcgg ccgccgctcc gagtcgctcg gctccggcgt
    20461 cgtcctcgac gcgtccggga tcgtcgtcac gaacgaccac gtcatctcgg gcgcctcgcg
    20521 gatcttcgtc acgacctccg acgggcgcga gctcgaggcg gaggtgctcg gcgcggacgc
    20581 cgacaacgac ctcgcggtgc tgaaggtcga cgggaagggg ctgaagccgg tccgcctcgg
    20641 ggcgacgggc gacctgatga tcggcgagac ggcgatcgcc gtcgggaacc cgttcggcct
    20701 ctcgaacacc gtgacgaccg ggatcgtctc cgcgctccag cgcaccgtgc gcggcgagac
    20761 gcggacgtac accgacttca tccagaccga cgccgcgatc aacccgggga actcgggcgg
    20821 cgcgctctgc aacgtcctcg gcgagctgat cggcatcaac accgcgatcg tcggcggcgc
    20881 gaacacgatc ggcttcgcga tcccggtgga acgggtgcgc cggatcgtcg acgacctcct
    20941 ccgcttcggc gaggtgaagc cggtctgggt cggcatccgc gggacgacgg tgacgccgga
    21001 gcgcgggcgg ccgagctcgc gcggcctcgg gatgcgcgtc cgctccgtct accccgcctc
    21061 ccccgcagag gaggccggcc tcgagcccgg cgacgtcgtc gtctcgctga acgggcggcc
    21121 cgtcgagtcg cgcgaggact tcgacacgct gctcgcctcg gtcccgcccg gccagtcggt
    21181 ctccgtcgag atgcgccgct ccggcgagct tcgcaagctc tcgctcgcgg tcgcccgcgc
    21241 cccggaggac ctcggcctcg aggtgctccg gcgggagatc gggatctcgg tcgcccaggc
    21301 ccgcggaggg ctcttcctca cgtccgtggc ccgcgagtct ccggccgacc ggaaggggct
    21361 cgagagaggg gactcgctcc tcgccgtgaa cgggaagcgt gtctcgacgc tcgacgacgt
    21421 cgcgcgcgcc gtcgagcggg gctggggccg gtcggggctc gtcctcgtcg tcgggcgggc
    21481 cggctacgcc tacacgctga cgttcggcct cgagtaagcg cggaccggag cgggccgccg
    21541 tccagcggcc cgctccgtcg ccgggctcac ttcccggtct tcgcgactcc cagctcggcg
    21601 agccgcttcg ccatgtcggg tcccgcggtc cccgccttca cctcgcggtc cacgtggagg
    21661 agcttcccgt ccttgccgat gtagaacgtc caccggcgcg ggtagccctt ctccgggtgg
    21721 aggacgccgt acgccttcgc gaccgatccc tcggcgtccg cgagcatcgc gtagtcggcg
    21781 tcgagggact tcgcgaactt cgtgatctgc tccagcgggt cgacgctggc cgcgaagtag
    21841 gcgacgtcga acgaccggag ctcctcgccg ctctcacgga gcgacttgca ctccagcgtt
    21901 caccctccgg tgaagacctt cgggaaccag gcgaggacga cggccttctt cccgacgtag
    21961 tcggcgaggg cgtgcgtctt cccgtccgac cccgggagcg agaaggccgg ggcggcgtcc
    22021 ccggcggcga ggggcttcgg ctcctcggcg acggcgagcg gcgccgccac gaggaggacg
    22081 gcggccgcga gcgtggcgag tctgcgcatg atcggctcct tgcggacgga tgctagccgg
    22141 gccgggccaa ccgggccagc ccgccccgct cccgtcccgc tatttccgaa ggcgcagctc
    22201 cgagacgacg atcccgagga ggacgagacc gcccccccac gcctcgcgcg ccgagagccg
    22261 ctcgccgagc gcaaccgccg cgaagagggc cgtgaagacc ggctcgaggg cgaagatgac
    22321 ggccgcgtgg acggccggca tccgggcctg cgcccaggtc tggacgagcg aggtgacgac
    22381 cgacgcgacg agacccatcc agaggacggc gaggaggagc tgaggcgtga atgcgagcgg
    22441 ccgcgcctcg gccgcgaaga aggcctccgg gcggtcgagg aacggggtca ggaggagccg
    22501 gcccgcaagg accccgagga acgcgaagac gatctgggcg gccgagtaga cccggacgtc
    22561 gtgcttcggc gaggcgactc cgagctccac gatgatcacg gcgtagacga gcgccgtgaa
    22621 gaagacgagg aggtcgccgg gatcgaggcc ggacgcgtcc ttcggccacg acatcagccc
    22681 gaagcccccc gcggccagga cgagccccgc gaggttcgtc ccgctcggca cctgccggtg
    22741 gaagagccag gcggcgaccg gcgtcagcgg cacggagagg ccggtgatga aggcggcctt
    22801 cgaggccgtc gtgaagagct gcccgacgag ctggcagccg atgccgacgg cgaggaggag
    22861 cccgatgagc gcgccgtccc ggaggactcc gggagtccgc ggacggccgc gcgcgaggag
    22921 gacgaggacc gccgcggcga gcccgtagcg aagggcgagg aagaggagcg gcggcgtctc
    22981 cttcaggacg agctcgttga cgacgaacgt ggagccccag atcgccgtca cgaggacgag
    23041 ggcggagtcg gcgaggagcg cctcgcgacg cggaaggtcc ggagcgctca cgaccggcga
    23101 tgatacgggt cggcgggagc ggcgcagtta tcatccccga ccgatgaacc cggccccgcg
    23161 cgtccgtttc gccccctccc cgaccggctc cctccacgtc ggaggggccc gcaccgccct
    23221 ctacaactgg ctcttcgccc gccgccacgg cggcactttc atcctccgga tcgaggacac
    23281 cgacgtcgac cggaacaagc ccgagctcgt cgaccagatc ctggaggcga tgacctggct
    23341 cgggatgacg tgggacgagg gaccgttccg gcagagcgag cgctacgacc tttaccggca
    23401 ggcggccgag aagctcctcg ccgaggggaa ggcctaccgc gccttcgaga cccccgagga
    23461 gctcgaggcg gcccggaagg cggccgaggc gcagggcgag acgttccgct actcgggcgc
    23521 gggtcgcgag atcccgaagg ccgagtcgga ccggcgcgcc gcctccggcg agccgttcgt
    23581 catccgcctg aagatgcccg acgagccgat cgtcgtcgac gacgtcgtcc aggggcgcgc
    23641 cgagttcccg gccggcgccc tcgacgactt cgtcctgatg cggtcggacg gccacccgct
    23701 ctaccacttc tcggtctgcg tcgacgacgt cgcgatgggg atcacgcacg tcgtccgcgg
    23761 cgtcgaccac ctcgcgaaca cgccgaagca cgtcgccctc ttccgcgccc tcggggccga
    23821 gccgccgaag ttcgcgcacc tcgggatgat cctcggcccc gacggcaaga agctgagcaa
    23881 gcggcacggc gcggccggcg ccgaggagtt ccgcgcgcag gggctcctcc ccgaggcgct
    23941 cgtcaacttc ctcgccctcc tcggctggtc gcccggcgag gaccgcgagc ggatgacgat
    24001 ggacgagatg gccgcgctct tctcgctcga gcggatcggc ccgtcgccct cgcgcttcga
    24061 ccacgagaag ctcgcctggc tgaacggcca gtacctgcag gacgtccccg acgcgcgcct
    24121 cgccgagctc gtgaagccgt tcggcgtgcc gggctcctgc cccgacgaga cgctcctccg
    24181 cgcgatccct cttcagaagc cgcgcgcgaa gacgctcgtc gagctcgcgc aggggctctc
    24241 ctcgtgggtc gccgacccgg cggactggga cgccgcgggg ctgaagaagt tcgggaagcc
    24301 cgcgcacgcg ccgctcctga agacgttcct cgcgcggatc gcggccgtga cgccgctgac
    24361 ccacgccggg atcgaggagg ccgcgcgcgc cctcgccgcc gaggcggggg tgaagctcgg
    24421 cgacctcgcc cagccggtcc gcctcgcgct gaccggcacc ctcgcctccc ctcccctctt
    24481 cgagatgatc gaggtcctcg gcctcgaaac gacccgccgg cgcgtcctcg cgctcgcggc
    24541 gcggatcgag cccgagccca ccccgagctg aggcgcccga tggaagagac gacgaccccc
    24601 gcccccgaga ccgccggcaa ggacttcatc cgcgagatcg tcgaggacga cctcgcgacg
    24661 ggaaagcacg cgaagatcac gacccgcttc ccgcccgagc cgaacggcta cctccacatc
    24721 ggccacgcca agtcgatctg cctcaacttc ggcgtcgcga gggagtacgg cggcacctgc
    24781 aacctccgct acgacgatac gaacccgctg aaggaggacg tcgagtacgt cgacgccatc
    24841 gaggccgacg tccgctggct cggcttcgac tggcacggca agtactacgc ctcggactac
    24901 ttcgggaagc tctacgactt cgccgaggag ctgatccgcc gcgggaaggc gttcgtctgc
    24961 gacctgacgg ccgacgaggt ccgcgccacg cgcgggacgc tgaccgagcc ggggaagaac
    25021 agcccgtacc gcgaccgacc cgtcgaggag agcctcgacc tcttccggcg gatgcgcgcc
    25081 ggcgagttcg aagacggctc gcggacgctc cgggcgaaga tcgacatggc ctcgccgaac
    25141 atcaacctgc gcgacccggc gctctaccgg atccgcaagg cgcaccacca ccggacgggc
    25201 gacgcgtggt gcatctaccc gatgtacgac tacgcccacg ccctctcgga ctggatcgag
    25261 gggatcaccc actcgctctg cacgctcgag ttcgaggacc accgcccgct ctacgactgg
    25321 tgcctcctcg cgctcggcct cgagagccgc ccccgccaga tcgagttcgc gcgcctgaac
    25381 ctgacctaca cgctcctctc caagcgccgc ctcctccacc tcgtcacggg cggacacgtc
    25441 cgcggctggg acgacccgcg gatgccgacg atcgcgggcc tgcgccgccg cggcttcacg
    25501 cccgaggcga tccgcgactt cgccgagagg atcggcctcg ccaagcgcga cagcgtcgtc
    25561 gacgtcgccc tcctcgagca ctgtcttcgc gaggacctca accgccgcgc cccgcgcggc
    25621 atggcggtga tcgacccggt gaaggtcgtc ctgacgaact ggcccgaggg gaaggtcgag
    25681 gagctcgagg cggtgaacaa ccccgaggac ccgtccgccg ggacgcggaa ggtcgcgttc
    25741 tcacgcgagc tcttcatcga gcgggacgac ttccgcgagg tgccgccccc gaagtaccac
    25801 cgcctcttcc ccggcagcga ggtgcgcctg cgctggggct acatcgtgaa gtgcgaggag
    25861 gtcgtcaagg acgcctcggg caacgtcgtc gagctgctct gcacgtacga cccgacctcg
    25921 aagagcggcg aagcgggcgc cgcgcggaaa gtgaagggga cgattcactg ggtctcggcg
    25981 gcccacgccg tcccggcgga ggttcgcctc tacgaccgcc tcttcacgaa ggaggacccg
    26041 aacgacgccc ccgaggggca ggactggctg acgaacctga acccggcctc gctcgaggtg
    26101 cggcagggct tcgtcgagcc ggcgctcgtc ggcgcggcgc ccggcgcgtt cttccagttc
    26161 gagcgactcg gctacttctc ggtcgatccc gacggcaccc ccggcaagcc cgtcttcaac
    26221 cgcgccgtcg gcctgcgaga cacgtgggcg aagatcgaga agaaggggcg gacggcatag
    26281 gcgggtccgc ccctgtcggg tcgaccgggc ggcaagtctc ctcgggggac gccgtgtcga
    26341 aggccccgca cggccggagg ctccttctct ggcttccggt cgccctgatg gcgggcgtcc
    26401 agctctggct ctcgtcgcga agccggctcc cggacgccct ctccccgttc ccgggctcgg
    26461 acaagctggc ccacgggggc tggttcttcc tcctcgcgct cctcgcctgg cgggccgctc
    26521 gggagggcga gggatggtcg cgccgccgcc ggctcggcgg gctccttctc gcggccgccc
    26581 tctgggctgc cagcgacgag gcgcaccagt ggttcgttcc ggggcgggcg gtggaggcgg
    26641 ccgacctcct cgccgacgtc gcgggcgcgg ccctcgccct ctccctcgcc gagggattcc
    26701 tcaccgggcg ccggctcgtc cgcgccggct gaaacgaaaa cgccgcccgt aggcggcgtg
    26761 tttcgtcagg ttggctgggg ggcagggatt cgaaccccga taggcagatc cagagtctgc
    26821 agtcctacca ttggacgacc ccccaacctg gaggggcggg cattatgagg gccggtcggg
    26881 cggcggtcaa ccgccgaccg tctcagcgcg ctttcttcga gagggaaagg aagaaccggg
    26941 cgaggtcctc cggtgtcccg cggccctgga acgtcacgga gacctctccc ttgacgggtc
    27001 cggccacgac gaccggctcc gcgtcagccg ccttcccccg ccccttcttc ggcgcggcgt
    27061 cgacgacctc gggcttcagg accttcgccc gtcccgcctt cttccggccg ttcttccggg
    27121 gacgccgggc ctccttcggg cgcagccggg tcacgtcggg cgggccgaga cggatgccga
    27181 acttctccgc gaccgcctcg atcacgaccc gcgcttccgt cgtcggggcc ttgacgagct
    27241 gcgcccgcgc ccaggcgagc gccgcgggac ggtcgatacc gagcttccgc cggacctcgc
    27301 ggatcgatcc cgcgccgatc tcctccggcg cgaccttcag ctgctttccg gcggccgcct
    27361 tgacgtcctt ctcgttcagc gacttgtcac ccttcaggag cgactcgacg acctggcgaa
    27421 gccgatcggc gtccacggtc atgggacctc cttttccgag ctcgcgtttc gcgagttcga
    27481 caattatgac ccaaaatggt cggttcggct cagtacctgg gaacgctcgg gtcgatccgg
    27541 ctcgaccagg cgaggatccc gccctcgagg ttcaccgccc cgccgatgcc gctcttccgg
    27601 aggaactgga ccaccgtggc gctccgctgg ccgctccggc aggcgacgac gacggtcgcg
    27661 tcgcgcggca cctcgtcgag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 ntgaacgtgc aggcctggac ctccgcgccg ggagcgacgt cgaggagacc ggcgcacttg
    27841 ctccccgagg tcgcctggac cgagtagctg aatcgcgccg tcgacgggcc tcccgcgcag
    27901 gtcgcggcgt gcttcacgta gagcgtgtag gtcccgaacg ggagccggtt cccctcgtag
    27961 acgacgctct cggtccggtt cccggcgccg caggcggccg gcagcctgcg tccgccctgc
    28021 tgcgtcgcca cgtcgacgcc gccggggccc gcgaggcgga cgtcgaggtc cgcgtcgccc
    28081 gaccagctcg cgaggccggt caggtactgc ggagtcccct gcgcgtcgac gcggatcgtg
    28141 gcgacggtgg cgttcgtcgc cgagccgccc ttcccgtccg tcacggtgag cgagaccgcg
    28201 aacgtcccgg ccgactggaa cgtgaagctc gcggtcgagc cgctggccga gccggcgccg
    28261 aagttccagc tgtaggtgag cgggtcgttg tcggggtcgg aggcggtcgc ggtcgcggtc
    28321 acctgaagcg ggacggagcc gtagagcggc gtcacgctca cggagccgat cgtcggcgcg
    28381 gcgttctcgt cgtcgctgct ctgcgacgcc gcgacggcca cgccggccgc gacgactccg
    28441 gcgccgatcc cgaagaccgt tccggcggaa aggccgcccg ccttcgccgc ggccgccccg
    28501 gccgccgcgc cggtccccga ggagagggcg ctcgagagcg acacgaccgc gccggtcacg
    28561 aggatgacct tcgcgatgtc gtccttgttg aagcccggcg gaaccggctc ctggcccgcg
    28621 cgggtcagcc ccacggtgag cccctcgcgc ggcgaggcgc tcgtgacgac cgcggtcgcc
    28681 gtccgctcgt cccggcagac cgtcttgtcc gtcacgggag gcgcgtaggc gggcgtcgtc
    28741 ttcggcagac tcgcgaggtc gagcgcctgg aggtagtagt cgatcccggg gatgccgggc
    28801 agcgggcgcg gcagctcgcc gacgaggtct tcggccgccc gcgccttcat caggacgtag
    28861 tagtagtcct cggtccccga ggcgcggaag tagacgaagc cctggtccag ctccttgccc
    28921 ggcaggacgc gggcgtcgac gagcggcctc gcctccgtgg agaggcactt cagcgggtcg
    28981 tgggcgatcg agagccgcgg gctctccccg ggcgcggccc tcttcacgac cgggtcggtc
    29041 tccggcgccg gcgcggcgaa ggctccggtc gcgagcgtcg cggccaggac gatcacgaac
    29101 gcctgtcgga gcggcagcgg gagctcgggg agcgcggcct tctgcttcac gggcggacct
    29161 cctcggttcg gcgggcgacc cgtccggcgc gtcgtcccga cgcgcgaaga aacggacccc
    29221 cggcgggtgt atccggtgcg aatcggttct ttcagttggt agggactata ccgccgaccc
    29281 gccgcgccgg gtcggtgcgc cggatcacgg gcagggattg atctcgaggg tgcgcgacca
    29341 ggagtcgccg gtgccgtccg tcaccttgat cgtcttgagg aacgaggcgc cgaggggccc
    29401 ctggaacgcc cagaccggct ccttccgtcc gacgcggacc ttcgccggcg gcggcgggga
    29461 ggtgaccggg cggaagttcg tgacgctcat cccgacgttc tcctcgacgt ggtcgagcgc
    29521 gacgtcgcgc tccgcctcgt tcgagaagta ggcctcgatc gtgacgagct gtccctgctg
    29581 cgggcggctc ggccggacct cgaactcgat gtagaagcgg cccaggtcgg gcttcgcggc
    29641 ccgggcctgc ggtccgctgc cgacgtcgaa ccccggggcg aacccgctgg tcttcccttg
    29701 cgcgaagctc gtcggcccca tctggacgca gaggcctccg cccgcggccg gcgtcgaagc
    29761 cggccgctcc acgacgatgc gggccgtcgg gcgcggcgtc gggacccgca cgacgatcgg
    29821 cttcgtcggc tcgacgcggg gcgtcggcag cggcggggcc tccgtcggga cgacggtcgg
    29881 ctgggggaac ggcgtcgccg gcggaaagac cgccgtcggc gtcgggagcg ggagaccggg
    29941 cgtcgggacg gcggtcggca gcgggagcgg aggcgtcggg acgggccgcc ccaggccggg
    30001 aatcagcccg ctcttgtaga gcagcgtccc gccgaggccg aggaagagga ggccggcgac
    30061 cgcgacggcg atcgggagga tcggggagcg caccggggcc gcggggcgcg ccggcgggcg
    30121 aggaggcccg cgccgcacga cgctcggagg ggccggcgcc gcaggctcgg cggcgaccgc
    30181 cggggggacc ggcgccgcga tctgcgccgt cgccttcgtc cgctccgggg cgacgggagg
    30241 catcgagatg ccgtgcgcct cgacgtgcgc cttctcggcg gacgcccggg cctccgaggt
    30301 gtcgagggcg accgtcccct cgtccaggga gagctcgccc gccatgccgg ccgagagggc
    30361 ggcggcctgg cggagcgccg cgtcgcgcag ctccgccatc gtctggaagc gcgcttcggg
    30421 cttcttgttg agcgccttca ggaccaggcc gcgcgtcacg agggagatcc ccttgttcat
    30481 gtcgcgcggg tcgggcggct cctgctgcac ctgcatgaac aggacggcgt ggctcgactc
    30541 ggcgacgaac gggacgcgcc ccgtgagcat ccggaagagg acgactccga gcgcgtagac
    30601 gtcgctccgg cggtcgacct tctgacccat cgcctgctcc ggcgacatgt agttcggcga
    30661 gccgacgagg aagccggtgc gggtcgtccc cccctcgcgg tcgatcaggt aggcgaggcc
    30721 gaagtcggcg accttgaccc gcccttccga cgtcatcagg acgttcgccg gcttgacgtc
    30781 gcggtggacg atccccttct cgtgcgcgta gtggagggcg tcggcgatct ccgcgacgat
    30841 cttcaggcag cgcgactcgg tgaggacctc cttcgagatg aggctgtcga gactctcgcc
    30901 gtcgacgaac tccatcacga ggtagatgag gttctcctcc tcctcgatgt cgtagacctg
    30961 gacgatgttc gggtggttca gcttcgcgac cgacttcgcc tcgaggcgga agcggcggac
    31021 gccgtccggg tcgccgatca ggtccgacga caggagcttg atcgcgacgg tccgctcgag
    31081 ggacggctgg tgggcccggt agacgacgcc cattccgccc cggccgagct cggcctcgat
    31141 gagatacttt ccgagctgct tcctcattgc tcgcccttga acccgatgac tgagtgtacg
    31201 tcagccgccg cggcgtccct gccccctccc ccgcccccgg gcgaggaggc ctggggacgc
    31261 gccgccgagg ccgcggtcga ggcgctccgg ctcgacccgc agcggacccg cgccgggctc
    31321 ctctgcgtcg ccgccggagc ggcggtcctc gtcgcgctcg tctccatcgt gcagggaggc
    31381 cgccgcgagc tcctgcgaac ggtcgaggcc gccggaccgt cgaacgtctt cgtccgcgcc
    31441 gagcgcggcg cgccgacgcc gctcaccacg gccggccttt ccgccgcccg ggcccgcttc
    31501 cccggcctgc gcgaggctac cggcgtccgc atcgtccccg cggccgtcca cggagggacc
    31561 ctcgagctgc cagcgacggt ctacggcgtc ggccccgagg cgctccagct cttcgagctc
    31621 cggcgggccc gcggccgcct cctcggcccg cacgatgccc gctcccgctc gcgcaccgcc
    31681 ctcctcggcg cgcggctcgc cgcgggcctc gcccgccacg gcgacccggt cggcacgcgg
    31741 ctgacggccg gcggcgagac gtacgaggtc gtcggcgtcc tcgagccctc cgcctccgtc
    31801 gccgggagcg gcggcgagct cgccggtgtc gagtgggact cggggctcct cgtcccgctc
    31861 ggggccgagc cagacgccgc gagcgccccg gaaggggaga tgccggtcga cctcctcgcc
    31921 ctccgcttct ccgacccggg ggaggcggcg cgcgccacgc gcctcctggc cccgctcgtc
    31981 cccacgggcg cggccgtcac gacgccgacg caggcgatcg agcagtaccg cgccgcccac
    32041 cgctcgttcg accgggtcgt cctcctcgtc tcggtcctga cggcggcctc cgccgcgttc
    32101 ggcgtcacga acctcctccg cgcgtcggtg cgggcgcgct cgctcgagat cggcctgcgg
    32161 cgcgccgccg gcgcccggcg cgaggacgtc cgcctccagt tcctcgccga ggggctcctc
    32221 ctcggcttcg gggggggcct cctcggcatc ctcctcggcg ttctcctctc cgtcacgctc
    32281 ctctcgcgcg ccgggtggac gccccacttc gccccgggca cgctcctcct cctcgcgggc
    32341 ggctcggccg cgttcggcat cgcggccggc atccgtcccg cgaacgaggc cgccgctctc
    32401 gacccggccg cgacgctgag gctcgagtga tcgctccgct cctcctcgcg gccctcctcg
    32461 ccgcgccgcc ggcgtccgag ccgctctccg tggaacgggc cgtcgagctc gcgctgaccg
    32521 gcaaccccga gctcctcgac gcggtcgacc ggtccgcgct cgccgcggcg aacctctccg
    32581 ccgcccgctc gcgctacctc ccccaggtca ccccctacct gctcccgtcg ttccggagcg
    32641 gtgacggcgc ggcggtgagc cgctacggcg tgagcgtcag cgagcagctc ccgttcgggc
    32701 cgcgcttttc cgggtacgcc gaggcgagcc gcctcgaggg ggaggagggc tgggattcct
    32761 tccattccct cacgctctcc cagcccctcc tgcgcgggct cgacccggcc gtcacgcgcg
    32821 agccgctccg cgccgcccgg cgccaggtcg ccgccgagga gcggaacctc ggcgttcaga
    32881 agcggctcac ggtcgtcgcc gtctggagcg cctacctctc cgcggccctc caggacgagc
    32941 tcgtccgcga ggcagccggg cggacgacgc gggcggagcg gatcctcgag gcgagccgcg
    33001 cgaaggagga gatcgggagc gtctcccgcc tcgacgtcct ccgcgccgag cagctcttcg
    33061 cccaggcccg ctcgcaggag aacgacgcgc gcaacgcgcg cgaggacgcc cacgacctcc
    33121 tggcccgcct cctcggactc ccggccggga gccgcttccg cctcgagccc ccgacggctc
    33181 tcccggtcga ggtcccgggg ctcgaggagg ccctcgacgc cgcggccgcg tcgcgcgagg
    33241 agctcgtcga ggcgcgagag cgggtgacgg acgccgaggc gcagctccgg atcgcgcgga
    33301 gcctcctcct cccctcgctc gacgccgggc tcacctggag cgcggcgggc cgctcggacg
    33361 gcttcggcgg agcgttcggc aatccgggcg gctcgacctg gacgctcggg ttcacctcgc
    33421 aggcgcccct caacctcggg gcgcagctct cggcgcggac ccaggccgag gtctcccttc
    33481 ggatcgcccg tcgggccgtc gagacgctcg aggcggacgt cgcccgcctc gtccgctccg
    33541 ccggccgccg cctcgccacc tcgcgcgaac ggctcgcgct cgacgaggcg aacgaggagg
    33601 tcgcacggat gcagctcgag gtggccgagc tccgcttcgg caaggggctc accgacaact
    33661 tcttcgtcgt cgacgccgag gggctctaca acgccgcccg cgtctcgctc ctcacctcgc
    33721 ggcgcgaggt cctcctcgac gggctccgac tcctcgccga ggccgggctc ctccgcccgg
    33781 aggcgttcct cccccggccg gcccccgcgc cgtacactcc gtccagccga tgatcgaagc
    33841 cgagtccgtc gcgccggccg tgcgccgcct ccgcgaggcg ttctcccgcc tcgccgggct
    33901 gccgccgcgg acgaaggcgc tcctcggcgg cggcgcggcg gtcgcccttc tcggggccgg
    33961 cctggtcctc cgccaggccg cggtttccga cgccgtcggc gaggtgcggg ccgtgcgcgg
    34021 accgctccgg atcaccgtcg ccgtcgccgg ggccctcgcc cccgcccggg ccgagagcta
    34081 cggccccgag atcccgggcg tcgagctgaa ggtcgtcgag ctcgtccccg aggggacgtg
    34141 ggtcgcgccc ggcgcgctcc tcgtccgctt cgacgacggg ccgttccgcc gcgagctcga
    34201 gacggcccgc tcgcgtctcg tccagacgtc gggagaggcc gagcaggcgc ggcaggcgct
    34261 ccgcgcgctc ctcgcgacgc agaggagcga gcggctcgag gccgagacgg cgttcgcgcg
    34321 cgccgagctg gacctctcga cgttcgtcaa cggcgtcgcg ccgctccagg tccagcagtc
    34381 ggcggccgcg gtcgagcggg cgaagcggga ggccgcggaa tcgacgcaga agctcgaggg
    34441 gctcgctccg ttcgccgaga aggggtacgt ctcccgggac gagctgcgcg ccgccgagcg
    34501 gcgggccacc gaggccgcgg ccgacctcgt cctcgccgag cagcagcacc ggacgctcac
    34561 gcgcttcacc cacccgcagc tcctggccca gaagagccag gaggtggcgt cccggcggaa
    34621 cgcgctccgc gtcgtctccg agaagacctc ggcccaggcc gcgcaggcga aggccgcggc
    34681 cgacctggcc ggcgcccgcg ccgcggaggc ggcggccgcc gtcgccgacg tggagcggaa
    34741 gatcgccctc tgcaccgtcg aggcccgctc gagcgggctc gcggtccacc gcgagatctt
    34801 cgagaagggg ggcgagaagc ggcgcgtgcg cgtcggcgac gccgtcttcg ccggccagcc
    34861 cgtcctcgac ctccccgacc tctcggagat gctcgtcgag acgcgcgtga aggagggcga
    34921 gatccaccgc gtcgcgcccg gccagccggc cgaggtccgc ctcgaggcct tccccgacga
    34981 ggtcttcgcc gcgaccgtcc tccgcctcgg cgccctcacg gcgggcgacc gcggcgaggc
    35041 ccgctccttc ccgctcgtcc tgaagctcgc gaaggccgac ccgcgcctcc gccccgggat
    35101 gaccgcgcag gcgctcgtcc tcgccggcga ggtggccgac gccgtccagg tcccgatcga
    35161 cgccgtccgc tacgagggcg acgcggccgt ctgcaccgtg aagaccgtca ccggcgccga
    35221 gacccgccgc gtgaagaccg gccgcagcaa cgcgttcttc gtcgagatca ccgaagggct
    35281 cgccgaaggg gacgtcgtcc tcctcgctcg gtaagggcga ggcgcggccc ccccgaccct
    35341 ggggagacgc tccgcggtca tcgcgcgctg cctatactcc cgtccgtgga ccgcgaacgc
    35401 tggagctcct atcccgccgc gaccgaggag gagcgcactc tcctcgcggt cctgaacgga
    35461 acggccgtgc gaaagaccct cccggtcgtc tccacccgcg gcgacgagcg ctccgtcgag
    35521 gcgacgttcg cggtcgaggc ggcggagccg ttcgacggga aggccgaggt cgtcgtccgg
    35581 ctcgtcttcc acgg
//
LOCUS       DAOONL010000029        35126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_982_length_35126_cov_18.012175, whole genome shotgun sequence.
ACCESSION   DAOONL010000029 DAOONL010000000
VERSION     DAOONL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 35126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35126
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_35126_cov_18.012175"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PLP50_06615"
     CDS             <1..1481
                     /locus_tag="PLP50_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPA51257.1"
                     /translation="PGAVDDAGNREANCAPMSRSFYLIAIGGTAMTPLAALLVEKGER
                     VFGSDLPLYPPMSDRLEALGIDVLPGFAARNLPPRVDRVVVGNLAGKDNPELVAALSR
                     GLPVASMPETLRTEFLAGRHPVVVAGTHGKTTTTALTAWLLAAAGRSPGYLVAGEPRN
                     FPSPSAIGTGEAFVVEGDEYSTSWEDKGPKFLHYAPRTFLLTSVEFDHADLYADLDAV
                     KEAFRAGVAIVPPDGRIVACGDDPNVRDVLGGARARVLLYGLGDAPDLDLRATAIEEG
                     PEGTRFVVEPRGRAPFAVLSPLAGRHNVANALAALAAGEGLDVPAERLAAGLPSFLGV
                     KRRLEVRAVAAGVTVVDDFAHHPTAVATTLAGARRRWPGQRVWGVFEPRSLTAGRADF
                     FGPYLAALRQADAVALAAPYHAARLSRPGAPGALDVETLVARLAAEGKEAFTAPDPDA
                     LREGLLSRLSEGDVVVVMSSGPFGGLCGKLVQALATREIAAV"
     gene            complement(1491..2255)
                     /locus_tag="PLP50_06620"
     CDS             complement(1491..2255)
                     /locus_tag="PLP50_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPA51258.1"
                     /translation="MRFLRYDDGGLPRWGRLEAGRVTPLDGAPWSGGEADGPARPYEE
                     LDLLPPVPPGGKVLCIGRNYLAHAKELGNEVPAEPLVFLKPSTSLLPHGGTVVLPPES
                     ERVDFEGELALVIGRRARRVGPEAFADVVFGATPAIDVSARDLQKKDGQWWRAKGFDT
                     FCPVGPVVAAAFDAADVRLRTFLDGEVKQDSRTSRMIFDLPTLVSWVSQAMTLEPGDL
                     LLTGTPEGVGPLADGQLLELEIEGLERLSVRVASER"
     gene            complement(2252..3118)
                     /gene="dapF"
                     /locus_tag="PLP50_06625"
     CDS             complement(2252..3118)
                     /gene="dapF"
                     /locus_tag="PLP50_06625"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPA51259.1"
                     /translation="MSAETIGGLPRTFRKMAGAGNDFLVFEGAANVGPREAETIRRLC
                     RRGTGVGADGVLFVSPREASGARREVRVDYFNADGGPARFCANGTRCAARFAVLRGLA
                     DAELVTHTGWGAVAATVRPDGTVTIRLPEPVALAGEIATFDPEGRVLEPRAAHLTVGV
                     PHLVVFCASGLEVETVDVATLGPPLRRHPDLPEGANVNFVSVRGGSRLSVRSFERGVE
                     AETLSCGSGVVASAVVAGAARGQLPPVSVATRSGSALVVDFRLEGSLAHDVTLTGDAR
                     VVFQGELTEEAV"
     gene            complement(3115..4197)
                     /locus_tag="PLP50_06630"
     CDS             complement(3115..4197)
                     /locus_tag="PLP50_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51260.1"
                     /translation="MRVGRTVLLLLAAALAAGCGRKIAPEAPLQVIPARPEPLRVSQE
                     GSDVVLRFPFPSKTIQGSPLTNLTRVTIYRELVPAPPGARAPEPADGAAEREREEKLF
                     RQRAEAIRDLPREGLDEITVGSELVVRDPMVPLFLEKRLGRVFLRYGVTATRDRKRVS
                     PLSPLVAIVPKVPPAEPVGLSATVEEGRVCLEWLPPAGMLDGSTPASVAGYAVYRREE
                     GEEAYGEWLGVAGRSPYVDTAVAVGKRYVYTLRAAPTADLPPVLGPAADEVVADTRDV
                     FPPAVPEGLLVLAEEGGNRCVWNPSLARDLAGYRVWARDGDGEWRRVADGLTDPAWYD
                     GGAPAGRRYAVTAVDAAGNESGRAER"
     gene            complement(4194..5519)
                     /gene="hslU"
                     /locus_tag="PLP50_06635"
     CDS             complement(4194..5519)
                     /gene="hslU"
                     /locus_tag="PLP50_06635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPA51261.1"
                     /translation="MRDLSPRRIVTELDRHVVGQAKAKRAVAVALRDRWRRQQLAPEV
                     AREITPKNILMVGPTGVGKTEIARRIARLVDAPFVKVEASKFTEVGYVGRDVESIARD
                     LVEAAIRLVRDERRERVAEKAQAAAEEKLVELLAAQVDPAGFVPGPDAKERLRRELRD
                     GKHEAREVEVEVVDERTPQISVMTPQGVEEMGIDLPGMLPNLFGGPKRKKARLPVSEA
                     RAVLVDREADVLSDEATVPQEAIRRAEETGIVFLDEIDKIAGREGKAGGPDVSREGVQ
                     RDLLPLVEGTTVKTKHGNVKTDHVLFVAAGAFHVARPSDLIPELQGRFPIRVELDALT
                     EADFVRILTEPEHSLPKQAQALLGTEGIELVFAEDGLAEIAKAAAEANRSLENIGARR
                     LHTILERVLSEASFAGPDAAGARVVVDRTAVTRALADLVASRDLSRFVL"
     gene            complement(5549..6094)
                     /gene="hslV"
                     /locus_tag="PLP50_06640"
     CDS             complement(5549..6094)
                     /gene="hslV"
                     /locus_tag="PLP50_06640"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPA51262.1"
                     /translation="MSETPAFHHTTVLAVRRGGAVALGSDGQVTLGTTVLKHGASKIR
                     RLAGGSVLAGFAGSTADAFALFTRFERKLEEFSGQLERAAIELATDWRTEKTLRSLEA
                     LLVVADREKTLLLSGTGDVISPDEGILAVGSGGPYATAAARALFRHTELGAEEIVRAS
                     LKIASEIDIYTNDAVSVETLP"
     gene            complement(6280..7191)
                     /gene="xerC"
                     /locus_tag="PLP50_06645"
     CDS             complement(6280..7191)
                     /gene="xerC"
                     /locus_tag="PLP50_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPA51263.1"
                     /translation="MSLAEALGAWDRHLADERAVSPHTRKAYVEDVARFLAFLGSFAG
                     KPAGETSPGEVDALAIRSWLASLRSEGQARTSIVRRLSALRTFFTFLQREGLAEGNPA
                     RGVATPRTDKNLPVALSVAEAAAVVEAPGADTEFGRRDRALLELLYATGLRVSELVGL
                     SLPDVDLPGRQVRTLGKGRKERIVPFGEKAAEALAAWLPARLALLGGRRVRGEPLFLN
                     ARGGRLTDRSVRRVLDQALSTAEVSRHASPHALRHSFATHLLSAGADLRTIQELLGHA
                     SLATTQKYTHLDVERLMEVYRKAHPKA"
     gene            complement(7188..8543)
                     /gene="trmFO"
                     /locus_tag="PLP50_06650"
     CDS             complement(7188..8543)
                     /gene="trmFO"
                     /locus_tag="PLP50_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPA51264.1"
                     /translation="MTRPRVTVIGGGFAGVECAYQLARRGHAVRLLEMRPARTTDAHE
                     TDRLAEMVCSNSFRSDNPGNAVGLLKREMEVVGSLVMEEARRAAVPAGDALAVDREIF
                     ARAVTARVESDPAIELVREEATALPDPGDGFVVVATGPLTSAPLAESLLARLGGDRLY
                     FYDAVAPIVEGSSLDLSVLYAASRWGKGGGADYLNVPLTREEYEAFVAALLAGETVPY
                     HDFEKAVHFEGCLPIEEMAARGPETLRHGPMKPFGLRDPRTGREPYAAVQLRQDNLAR
                     SHWNLVGFQTKLRVGEQRRVFRTLPGFANAELVRYGMLHRNTFLNGPAHLDRLFRFKE
                     DPRVLFAGQVTGVEGYLESAATGLMAGAALAQLLEGREPLPLPFSTALGALSRHVSES
                     DPEHYGPMNVTFGLIDDSALPPIRDRAKRREEIVRRALAEVAAWRDAALGPAPEAAGA
                     A"
     gene            8598..9302
                     /locus_tag="PLP50_06655"
     CDS             8598..9302
                     /locus_tag="PLP50_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA51265.1"
                     /translation="MTFERALYDEAYWRGLHPHHWFKNPPRKYAERDRDVLRVVAPRP
                     SDVVLELGSARGDVTFLLSRHAREVIGVDAAPEAIALAEGARSRLGIGNVRWLEADVA
                     DLAPVPDGSVDAVAAIDLVEHVDDPTLEAMLRECRRVLKSGGRLGIYTPDRAHYVERM
                     KAHDFVLKQFPQHIAVRRVAEYRRFLAAAGFAVDLETWSVSPFPLVRWVERALAPLPL
                     LGPTFRYRILIRALAP"
     gene            9313..10023
                     /locus_tag="PLP50_06660"
     CDS             9313..10023
                     /locus_tag="PLP50_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA51266.1"
                     /translation="MPEPPDFAPNAAVYDDAYYAAMYRPHWFFRNARKYRERDAALLR
                     AVRPHAGMRLLEVGSARGDTAFFFAPLVAAVVGVDAAPVAVEAARAAAAARSLANVTF
                     EVADARRLPGIADASFDAVLLADFVEHVTDEVLVPCLAEARRLLVPGGALAIYTPNRD
                     HWAERIKAGVPGLQQEDHIAVRPSAEVVRLVAGAGFSVDDLFFSASPYPLLGAVDRLL
                     PGVAICRFRTVLRALRTA"
     gene            10121..11689
                     /locus_tag="PLP50_06665"
     CDS             10121..11689
                     /locus_tag="PLP50_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HPA51267.1"
                     /translation="MSRRSLLLFLLLAALIPGADAAAQNTGGVVLYEGTRLTGSRIDL
                     DRDVFDLDETAFRSRRARSVAVASGCRATLFELSGYRGASVEVTGRIDDLSATRLGAR
                     GVSSARVACTRDLVGSSSAPWAAGSRGVTLFRDSGFKGDSETFSSDVPDLSRTRVGAR
                     QASSVDVPEGCVAVLFSGTGFSGRSTELRKSHDNLRLAEIGNDTVASLRVDCRGTGGS
                     TIDSGWSAGGRGVTLFRDSGFKGDSETFSTDVPDLSRTRVGARQASSIEVAEGCTAEL
                     FTGTGFSGRSAVFREAHDNLRLTDVGNDAAASLRVDCRGGYGLAGAGRRPGASGRGVT
                     LFRDSGFKGDSETFSTDVPDLSRTRVGARQASSIEVAEGCTAELFTGTGFSGRSAVFR
                     EAHDNLRLTDVGNDAAASLRVDCGRRRFRPADGRGAGYGSAGVTLFRDKDFDGEAESF
                     RSDVPDLARTRVGGRQASSIEVPAGCRVTLFSEPSYRGRSATFDADHGDLRQTPIGND
                     AAQSMRVDCGSTRW"
     gene            complement(11726..14344)
                     /gene="topA"
                     /locus_tag="PLP50_06670"
     CDS             complement(11726..14344)
                     /gene="topA"
                     /locus_tag="PLP50_06670"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPA51268.1"
                     /translation="MSKSLVIVESPAKAGTLGKFLGKDFTVHACYGHVRDLPRKGISI
                     DREAGYEPTYEVLPGKQRTIADLKRLAKGAEAIYLAADPDREGEAICWHLHELLRPAA
                     PNARFHRAEFNEITKSAIQKAIASPGEIDANRVAAQQARRIIDRLVGYEVSDLLWKKV
                     WRGLSAGRVQTVALRIIVEREAERERFVPVPYFSVPLQLAKGDAPFPARTVAWRGAKL
                     KFDGTDPRLATREAAEEVLGHLRAATFRIASVEAREKRTNPVPPFTTSKLQQGAARTL
                     GFTVRRTMQVAQRLYEGRTIAGRGSVGLITYMRTDSTRVSEEALAAVRAHIGATYGAD
                     ALPSEPRRFKQKKDAQDAHEAIRPTMMDLPPEVVAGDLSADEAKLYRLIWNRFVASQM
                     APSVSDVTTVEIEATRPGSTEVAGVRASGSVLKDPGWLRVYGQVAEGEEAEGEGNGDP
                     EEGKVRLPQLGEGDVLRLVEGQVEDHETQPPPRFNEASLVKFLEENGIGRPSTYAEIL
                     RKIEDREYVRKKDRRFLPTLLGRLVIDLMREGFDDFFQTESTARMEEELDEVEEGKLD
                     WRKAVGEFDARFTKDRDRALKKMISVKAGLALAEVRKRLPGFPLPEGVGEKCPKSGDE
                     LRLRMGKNGLFVACAGYPGCDYTVDVPEPEDDPVDASELEGQTCDECGSPMKLRTGRD
                     GSAFLGCTAYPNCRFTVAVKVANGKAEARPDVPTGEKCPLCGHDLVARHGRFGDYVGC
                     SNYPACRYRPPKPVTSTNVACPDCGTGEVLVRKGRFGPFYGCSNYPTCTRNFRARPVP
                     KACPACGAPYLLVRDRKAGAFYVCEREGCDFDAPAQDLDAWAFVTKVPEATALAAAAA
                     AASEKKRPAAKRARKG"
     gene            complement(14370..15485)
                     /gene="dprA"
                     /locus_tag="PLP50_06675"
     CDS             complement(14370..15485)
                     /gene="dprA"
                     /locus_tag="PLP50_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPA51269.1"
                     /translation="MRMQADIVLSWGLRVRGTRARTLARLLLATGSVEKTLGLSPAEA
                     VALGGEPGCEELLVPPSADGLAAARDGLGALGGRAIAICDPEYPALLREIADPPPVLF
                     VRGRTLSNAPVVGVVGSRRATRTGLEAARRLSAGLARAGVVVVSGFAHGIDAAAHAAA
                     LDEDGETWGVLGCGLDVLYPAAHGRLRERVFAGGAFLTELPPGTQPEPWHFPARNRII
                     AGCARLVVVVEAAPRSGSLITARCATEAGRDVGAVPGPVLGEQSAGTNALLKDGAILV
                     RDAADVLRELPDADLARLRPAARPEAGGATATPADPDAARVLLALDAVEPRPADELAA
                     VTGLSAARLSAALVLLELEGLAEALPGALFARKAPRA"
     gene            15531..15920
                     /locus_tag="PLP50_06680"
     CDS             15531..15920
                     /locus_tag="PLP50_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA51270.1"
                     /translation="MRHSRRAPLAKGLLLAIAVSLTGCASASRDAANAADQKAYGARM
                     AEKGFWREALFRFERAAKLAPDDPEVLNNLAVACEATGETARALAAYKRALELRPEDS
                     RIKRNYARFAEYYTSLQRASGPMAAPK"
     gene            15917..16621
                     /locus_tag="PLP50_06685"
     CDS             15917..16621
                     /locus_tag="PLP50_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51271.1"
                     /translation="MRRLSALAALLLLLGAPAGPLLAGSREVKLRLPLRPKLAVTGRE
                     RLSLAPFLVASKLSERKESPRVKDLDLDLEFRRYLGKQIARRTKMNVVTMPADVKLPS
                     TELKALGEASAYWKELGTRVGADVLLSGIVDFRVEDKSGYKTQEYQSPVDGRTYYRQV
                     FVETTGFTFEVTVLAFDGRTGAKLFQETFKDQKEKSRRAADEMVGLFENLFSLENQLL
                     GLFVVREREANRYVFD"
     gene            16608..19484
                     /locus_tag="PLP50_06690"
     CDS             16608..19484
                     /locus_tag="PLP50_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPA51272.1"
                     /translation="MCSTRRRAARLVPAGALAALLLVTAGPAPAQGQNKVVYDRFDWK
                     IYSSTHFRIYYYGKGEEALGKVASMAESAYDDLSRRLNYQVPKPIPLLYYVSHAEFEQ
                     NNIILNFIPEGVGAFAEPARNRMVLPIDLPDDKLQNLIAHELTHIFQYEILFGGRLSR
                     AITANIPTWFMEGMASYFAKDEDEKDRMFIRDAVNSDQIPPITRINITGYPAYRFGHA
                     FFDFVEAEWGKDAVRELVFEFRSFLGRDVAVALRRTFDVEPDDFDLKFRRYLRRKYLP
                     LLAVKGEASEYGERFRVGPWERPSYEVGVAPSPSGDFVATLTTYKEDVDIALLSTKSR
                     KLWKNLSSGRTTKYEYISAQFLTQAPESGRDLSFAPDGDRIAVFARRERGRQLYIFSA
                     RGGGILERIPVSPDMPVGPSFSPDGRSVVFSGISGTSRDIFRLDLETKAVTNLTSDEA
                     YDTAPVYSPDGKYLYYTKSVGELQKIVRFPVDDPTKVEQVTWGEGNDDDAALSPDGER
                     LYFASSRGGIFNVYGQDLKTGEIVQYTDVIGAAFGPAAYIGPDGQEKVVFSGYQAGRI
                     QLYVADAKKPFRRLEEKALSPAPVDPAAGPGFVPAIEVAIDHEKVEKSRFALFIDDVG
                     AYAGVQSDGTFVSSARVSLSDYIGDRRAFVIWDTLAGYSNFNAIYFDLKRRLQWGLQA
                     YDSRIYFLGYNQIGELTSDRRFLRETGVNVLGSYPFSRYTRVEGSLGFVSRSIDAPYA
                     YTNADGTQGQIVLPRKDNTMAAGLGFGYDATQFKAFGPHGGRALSVNYRYLPDFEDGG
                     TLSQEITAEARLYVPVTRRSNFAIRAFGGAASGASPAVYAFGGLDNLRGYDYRSFIGN
                     RIAYLNAEFRFPLIDALITGIGLNVGGVRGRFFFDVGGAWLEGEDFKWWDDGRLRDGR
                     AAWGAGFTAYFLGIPWNVDFARPFDGRSNLAGWQTTFYIGPGSF"
     gene            19552..20313
                     /locus_tag="PLP50_06695"
     CDS             19552..20313
                     /locus_tag="PLP50_06695"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HPA51273.1"
                     /translation="MKVRVLGAYGGSSPWHRQTSFLLDGTVALDAGALTQSLTLEEQS
                     RVRSILVTHSHLDHVASLPFLVENVFGRTEGPIEIVGPKDVTTLLQGHLFNDAIWPDF
                     TRIPTHLLPAVTFRAVEEGTPFRVDGLHGLAVAVSHVVPTFGYLLWDDRACVVFSGDT
                     GPTEALWAAARSRDDLKSIFVECSFPDSMAEIAEVSRHLTPATLALEMRKFPAGIPVN
                     LYHMKPPTLPQLRADVAALSEPRVRILDDGDELEF"
     gene            complement(20582..21694)
                     /locus_tag="PLP50_06700"
     CDS             complement(20582..21694)
                     /locus_tag="PLP50_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51274.1"
                     /translation="MSAVREALEALGHPPGALPEARLLGDLFVRFQAQVALRSAPDGS
                     AEDHLSAWLESGTGLCGEARTAAFAALAAEAGFAIGPARARGAAGDVRTILLAEDGRL
                     LLDVSFPLPAPVPLEPPARELATGYGALSVRRGSDGPGLHLETRGEERLLYSVDPGPG
                     AASADTTKEPPREPGLFRLLDDRLLRWKGGVLEVSDAWSRLRVPFPASDDDGLEALFG
                     PISPPEDERWATADPPTLAVYLASEARPEAFRALLGDPESFARTLPEEWSATAVEMRA
                     DGYSWVVREAEETLRTERVRVVPDGLVIEAEGPLALFRSRTLRLQRRTDGTRLRLLGV
                     LRDPVPPRGLPDGTRRRLVFELANELLALDRVAAGS"
     gene            complement(21703..22293)
                     /gene="pdxT"
                     /locus_tag="PLP50_06705"
     CDS             complement(21703..22293)
                     /gene="pdxT"
                     /locus_tag="PLP50_06705"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPA51275.1"
                     /translation="MRVIGVLALQGDFEAHGAALARLGVDSRPVRTAEEVRASAGLVL
                     PGGESTTMWKLMEGTGIAEAIVAVARRGDPLFVTCAGAILAARRITSPDRNGLGLLDA
                     TAVRNAYGRQLDSAVVTLTDLDRSVLGSEPLEAVFIRAPKLADLGPGVEVLARRNGDP
                     VLVRDGNLVAATFHPELADDVRVYDLVFGGAAARAA"
     gene            complement(22290..23201)
                     /gene="pdxS"
                     /locus_tag="PLP50_06710"
     CDS             complement(22290..23201)
                     /gene="pdxS"
                     /locus_tag="PLP50_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPA51276.1"
                     /translation="MSSTPVFSTPFTSDAFRVKVGLAEMLKGGVIMDVVNAEQAKIAE
                     DAGASAVMALERVPADIRRDGGVARMSPVSKIREIEAAVSIPVMAKCRIGHIAEARVL
                     ESLEVDFIDESEVLTPADEENHVYKHDFKVPFVCGCRNLGEALRRIGEGAAMIRTKGE
                     AGTGDIVHAVKHLRAVTRQMKSLTLLSRDEWMAAAKQLAAPFELVVWVAEHGKLPVPN
                     FAAGGVATPADAALCRILGAEAVFVGSGIFKSDDPANRARAIVEATTHWDDPARVAAA
                     SEKLVEGMKGIEAGKLVESELLQTRGW"
     gene            complement(23214..24719)
                     /locus_tag="PLP50_06715"
     CDS             complement(23214..24719)
                     /locus_tag="PLP50_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPA51277.1"
                     /translation="MTDRPSAADGAPGRIPLYRTIADGIIRDIRRGRFQEGSRLPASR
                     ALARTHEVNRATVHQALQVLKREGWIETNKGGGTRVARRSLAGPMVGPAPSWDRISSA
                     LLGRMLAEAGVPDGPLDGAHDLARLAPEEKEFPAEAFAKVLADVARDGALLAYGSPFG
                     YRPLRETLAARLERRGVPADADALLIVNGAQQGLDLIGRALVDPGDAVAIESPSYSGA
                     LSLLRAQGARLLGVPLDAEGPAMEPLRSALAERPKFFYTIPDFQNPTGLLATAARRRE
                     LVAAATAAGVLVVEDAFDADLLVEGELPPPLAALAPGHVVHIGTISKALFPGVRVGWI
                     SGPREFVHRLAALKRIGELTSPPVLQAALNVFLSRGDYDRHLLRVKARLKARLALAHK
                     TVLRSFPVGTRCERPLGGWVLWVDLPEDVSGRELAEAARGAGVLVSPGGDYAPDRADV
                     PSLRISVARSSGAELEKALESLGVLARAAVRRAHRRAPRAEERREEALIQI"
     gene            complement(24804..25088)
                     /locus_tag="PLP50_06720"
     CDS             complement(24804..25088)
                     /locus_tag="PLP50_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPA51278.1"
                     /translation="MALSRERANDLSHRVLDALRKTPGVELTAEAEFVRNRIASALQE
                     WEKESDGLSAEARRRVLARGRRVPEGSREFDQLLSEELRRLWDERAARGE"
     gene            complement(25184..25837)
                     /locus_tag="PLP50_06725"
     CDS             complement(25184..25837)
                     /locus_tag="PLP50_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPA51279.1"
                     /translation="MRFFLDTVDADEARRAKEWGLLDGAVVRADAVLAAGRDYRKAVS
                     EISQVCDGPVFAAVAAGEPKGMYKEARELAKLGKAIVVRLPMTREGLRVVRLLNDEQI
                     AADVHLLATPLQALLAARAGAAYASVAVSGLDGAGQVGMDVVDAVVRIYDNYGLQTQI
                     VVDAVQNPVHALDAAAMGADAAAIPYPVLDRLFDHPLTAQGVAAIRAHAGEGPRRVH"
     gene            complement(25842..28463)
                     /gene="gyrA"
                     /locus_tag="PLP50_06730"
     CDS             complement(25842..28463)
                     /gene="gyrA"
                     /locus_tag="PLP50_06730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPA51280.1"
                     /translation="MTDSPAPPPAPTEQRIPVSIEEEIRRSYLDYAMSVIVGRALPDV
                     RDGLKPVHRRVLYGMWEQGNRHNRPYKKSARIVGDVMGKYHPHGDASIYDTAVRMAQD
                     FSMSEPLVDGQGNFGSVDGDSAAAMRYTEVRLTALAEELVGEDIDKETVDWSPNYDGS
                     LTEPNVLPAKFPNLLVNGAEGIAVGMATKIPPHNLREACDAAILLLDRPDAGLDELLR
                     ALPGPDFPTGGILLGRRGVVEAYRSGRGIIQVRGRAEIEASTRADRESIVITEIPFQV
                     NKARLIEQIADLVNEKRLEGISAIRDESDRDGMRVVVDLKKGENANVLLNRLYQLTPL
                     QSSFGIIFLAIVDGQPRVLPLKDMLRHFLDHRRQVVVRRTRFDLAKAEGRAHILLGLA
                     LALSNLDRVIAIIRGSKDPEEARRRLTEEVAVDLPALLRFLDLPALDLSPRARRDGEL
                     VRLDDVQAQAILEMRLQRLTGLEREKIVAEYKDVLALIADLKDILARPERVAAIIRAE
                     LTEIRTRFGCDRRTEISTLERDLADEDLIKDEAVVLTVTRGGYAKSTPLSVYREQKRG
                     GKGRTGAAATEEDVVEHLFVASTHDSLLVFTDRGRVHCVRVWDVPSAGPAARGKALVN
                     LIQVEPGDTVAAMTTTRGFPEDRFLLFATKRGLVKKTVLSAYGNVRAAGIIAINLEEE
                     DELLSVQVTDGSRQVFLGTRNGMGIKFTETDVRPMGRDTTGVKGIELREGDEVVEMDL
                     VDEEATLLAVTELGYGKRTDVAEYRHQTRGGTGVINVRVTDKNGPVVGIKSVGEDDQL
                     LVITRMGMMIRFAAAGVRETGRAAQGVKVIDLDEGDTVVAVAKLASLGERDEAAEDDD
                     AESPVAPSGDEPESELE"
     gene            complement(28673..31138)
                     /gene="gyrB"
                     /locus_tag="PLP50_06735"
     CDS             complement(28673..31138)
                     /gene="gyrB"
                     /locus_tag="PLP50_06735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPA51281.1"
                     /translation="MMSSPPEPDDVSLEGRESSYGTESIKLLKGLEAVRKRPGMYIGN
                     TDDITGLHHMVTELVDNAIDEAQVGYCDRVTVTVHADETVTVEDNGRGIPVGLHPVHG
                     RETLEIILTDLHSGGKFDDNAYKVSGGLHGVGLSVVNALSATLDVEVRRGGRVWQQPY
                     AKGKPLEPIREVGRTNKTGTKLTFRADPEIFSVTAYNFDALSQRMRELAFLNPGVTIE
                     VFDERDETGEKKHVFRYEGGISSFVEHLNKNRERLHENVIFFTDVKDPSGDPVQDAKN
                     ERLDVALQWNDGYQEQIYCFTNTISNKDGGTHLEGLKAALTRAIQKYAEANNLTKNLK
                     ETNLSGDDCREGLTAVVSLKIRDPKFSSQTKEKLVSQEAKTWVQTVTYEKLSAYFEEN
                     PKVARRIVDKIIEAARAREAARRARELVRRKGALDGAGLPGKLADCQEADPALSELFI
                     VEGDSAGGSAKQGRDRRTQAILPLRGKILNVEKARFDKMLAATEIRSLILALGAGIGE
                     EFDVEKIRYHKVVLMTDADVDGSHIRTLLLTFFFRQMRAALERGYIYIAQPPLYKVVD
                     GKRESFLKDDKEYARFLLSRIGDDRVLVTEPSGVSVSGAALVRVFTEAQEWLSLVKRL
                     SLRGVPEELLRALVKAGLGPEAAREKGVLEALGAELPAELSPEVHADEEHGGFALHVA
                     LEVNGVVRKTIVDAAFLGAFDIRRAGELAAALDGFLDGPYLLRRNDGERRVATLVEAA
                     EAILESAKKGLAVSRYKGLGEMNPETLWETTMNPESRRLLQVRVEDAVEADELFTILM
                     GDAVEPRRAFIEANALAATNLDI"
     gene            complement(31119..32291)
                     /gene="recF"
                     /locus_tag="PLP50_06740"
     CDS             complement(31119..32291)
                     /gene="recF"
                     /locus_tag="PLP50_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPA51282.1"
                     /translation="MIGTLSVESFRNLAPARLGFHPRLNLLVGANGQGKTNVLESLAL
                     VSGRASFRTADLADVVREGANDATVTVGLVGESAGELAARIGKGRREHFWNGRKVSRV
                     EASRRLPLVVLTAADLGRLGGPPSDRRRALDRVAVAHEPALAAEHRRYEKARADRVAL
                     LAAPGRPDRDALAAFEEILASAGAAIAASRRRWVGPLGRRVEENARLLGLPYREISFR
                     LVSELPAHGSLPLLADALRSGLRQREESERRAGRALFGPHRDDLVLLFEERPLAPRAS
                     SGEARALVLAWALAERGLLAEAGGRLPVFAFDDFDSEWDPDVLTRFARALPDDGQVLL
                     TSARERVARELPLPAGAVWRVEAGRLAPSGPAAEPAAFVPAPARWPGERSDDVLAS"
     gene            complement(32313..33437)
                     /gene="dnaN"
                     /locus_tag="PLP50_06745"
     CDS             complement(32313..33437)
                     /gene="dnaN"
                     /locus_tag="PLP50_06745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPA51283.1"
                     /translation="MELTLPAATLAREMSLMQGVVERRTPHQILSNVLLEARDGMLTL
                     TATDLDTTFVSEIEPLSLSVPGAVTVPARKLFDVVRDLPAAAEVRLRVLENHHVQIDC
                     KAPKSRIRLNGLPATDFPTRPEATGGPTLSLTFGALRRMVEKVRFAVSTEEIRLQLQG
                     ALLKPKGGGLEMAATDGHRLALIEVEDAQAEAGFEAILVSKKVLDELSRLDAEDETPV
                     TIVRGTNHIGFTLGRRRIFSKLDDRRFPDYEKVISKENTKRATVVREELLGAVRRISL
                     LSGDRLKAVSLSFREGALVVSSESQDVGDGDQEIGAEYEADPVTLRLNSRYLEQFLEA
                     CETEKVHLFVKDEGSQCQGRPAEPVPGLKSYLYVVMPMRV"
     gene            complement(33660..34976)
                     /gene="dnaA"
                     /locus_tag="PLP50_06750"
     CDS             complement(33660..34976)
                     /gene="dnaA"
                     /locus_tag="PLP50_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPA51284.1"
                     /translation="MEKPTSWEKILDHLKSRVDERDFDTWFRGTRQKGETQDAVFVLI
                     SSPLFIDYIPREYGDQIAEAARLAGLGTREIRFVLEGRDDARAVVSPARPQPNRSRVG
                     DLNPAYTFEHFVIGKPNQLAHAAALHVADQTSHRYNPLFICGPTGVGKTHLMQAIGHR
                     RLSRRPGERVLYVYSETFVKEVVTGIRFNRMDQVRQRLLNADVLLFDDVQFLAGKATT
                     EDELFQTFNALYSIGSQIVFTSDVLPRDIPGLTDRMKSRFEGGLVVDIQQPDFETKVA
                     ILRHKAEIARVELDDDTAYYLSGKIKTHVRELESYFNRVVLVSSMRGEPITRDLAEEA
                     LRGILPDDSSKTSPAEILKAVAAHYGLKPAELRARTKREKIVFPRQVAMYVLKEAIGL
                     SLPEIGRLFGDKHHTTALHSIKKISARREEDPDFDREVAVLVAQFR"
BASE COUNT         4708 a        12699 c        12606 g         5113 t
ORIGIN      
        1 atcccggcgc ggttgacgat gccgggaacc gggaagcaaa ctgcgcgccc atgtcgcgca
       61 gcttctacct catcgccatc ggcggcaccg ccatgacccc tctcgccgcg ctcctcgtgg
      121 agaaggggga gcgggtcttc ggctccgacc tgccgcttta cccgccgatg tcggaccggc
      181 tcgaggctct cggcatcgac gtcctgcccg gcttcgccgc gcggaacctc ccgccgcggg
      241 tcgaccgcgt cgtcgtcggg aacctcgccg ggaaggacaa ccccgagctc gtcgccgccc
      301 tttcccgcgg cctcccggtc gcttcgatgc ccgagacgct ccggaccgag ttcctcgccg
      361 gccggcaccc ggtcgtcgtg gccgggacgc acgggaagac gacgacgacc gcgctcacgg
      421 cctggctcct cgcggccgcg gggcgctccc ccggttacct cgtcgccggc gagccgcgga
      481 acttcccctc tccctccgcg atcggcaccg gtgaggcgtt cgtcgtggag ggggacgagt
      541 actcgacctc gtgggaggac aaggggccga agttcctcca ctacgccccg cggacgttcc
      601 tcctcacctc cgtcgagttc gaccacgccg acctctacgc cgacctcgac gccgtgaaag
      661 aggcgttccg cgccggcgtc gcgatcgtcc cgccggacgg ccggatcgtc gcctgtggcg
      721 acgacccgaa cgtccgcgac gtcctgggcg gggcccgcgc ccgcgtcctc ctctacgggc
      781 tcggcgacgc tcccgacctc gacctgcgcg ccaccgcgat cgaggagggg cctgagggga
      841 cgcgtttcgt cgtggagccg agggggcggg ccccgttcgc ggtcctctcg ccgctcgcgg
      901 ggcggcacaa cgtcgcgaac gcgctcgcgg cgctcgccgc gggggaaggg ctcgacgtcc
      961 ccgccgaacg gctggccgcg gggctcccga gcttcctcgg cgtcaagcgc cggctcgagg
     1021 tccgcgccgt cgccgctggc gtgaccgtcg tcgacgactt cgcccaccac ccgacggccg
     1081 tcgccacgac gctcgccggg gcgcggcgac gctggcccgg ccagcgcgtc tggggcgtct
     1141 tcgagccccg gtcactgacg gccggccgcg ccgatttctt cgggccctac ctcgccgcgc
     1201 tccgccaggc cgacgccgtc gcgctggccg cgccgtacca cgcggcgcgc ctctcccggc
     1261 ccggcgcccc gggcgcgctc gacgtcgaga cgctcgtcgc gcgcctcgcg gccgagggga
     1321 aggaggcgtt cacggcgccc gacccggacg ccctccgcga agggctcctg tcccgcctct
     1381 cggaggggga cgtcgtcgtc gtcatgtcct ccggcccttt cgggggcctc tgcggcaagc
     1441 tcgtccaggc gctcgcgacg cgggagatcg ccgcggtctg acgcgggccg ctaccgctcg
     1501 gaggcgaccc tcaccgacag ccgttcgagg ccctcgatct cgagctcgag gagctgcccg
     1561 tcggcgagcg ggccgactcc ttccggcgtc cccgtcagga ggaggtcgcc cggctcgagc
     1621 gtcatcgcct gcgagaccca cgagacgagg gtcggcaggt cgaagatcat ccgggaggtc
     1681 cgggagtcct gcttcacctc gccgtcgagg aaggtccgga ggcgcacgtc cgcggcgtcg
     1741 aacgcggccg cgacgaccgg gccgaccggg cagaacgtgt cgaacccctt cgccctccac
     1801 cactggccgt ccttcttctg gaggtcgcgc gccgacacgt cgatcgcggg ggtggcgccg
     1861 aagacgacgt cggcgaacgc ctccggcccg acccgccgcg cgcgccggcc gatcacgagc
     1921 gcgagctccc cctcgaagtc gacccgctcg gactccgggg ggaggacgac cgtacctccg
     1981 tggggcagga gcgacgtcga cggcttcagg aagacgagcg gctcggccgg gacctcgttc
     2041 ccgagctcct tcgcgtgggc gaggtagttc cggccgatgc agaggacctt gcctccgggc
     2101 gggaccgggg gcaggaggtc gagctcctcg taaggccggg ccggtccgtc ggcctcgcct
     2161 ccggaccagg gggcgccgtc gagcggggtg acccggcctg cctcgagccg tccccagcgg
     2221 gggaggcccc cgtcgtcgta tcgcaggaac ctcataccgc ctcctccgtc agctcgccct
     2281 ggaagacgac ccgggcgtcc cccgtcagcg tcacgtcgtg cgcgaggctc ccctcgagcc
     2341 ggaagtcgac gacgagcgcg ctccccgagc gcgtcgccac ggagacgggc ggcagctgcc
     2401 cgcgcgcggc ccccgcgacg accgcggagg cgacgacgcc ggaaccgcac gagagcgtct
     2461 ccgcctcgac gccgcgctcg aagctcctca ccgagaggcg cgacccaccc cgcacggaga
     2521 cgaagttcac gttcgccccc tcggggaggt ccggatgccg gcgaagcggc ggaccgagcg
     2581 tggccacgtc gaccgtctcg acctccagcc cgctcgcgca gaagacgacg aggtgcggga
     2641 cgccgaccgt caggtgggcg gcgcgcggct cgaggacgcg cccctccggg tcgaacgtcg
     2701 cgatctcccc cgcgagcgcg accggctcgg gcagccggat cgtcaccgtg ccgtcgggcc
     2761 ggacggtcgc ggcgaccgct ccccagccgg tgtgcgtcac gagctcggcg tcggcgaggc
     2821 cgcgcaggac ggcgaaccgt gccgcgcagc gcgtcccgtt cgcgcagaag cgcgccggtc
     2881 cgccgtccgc gttgaagtag tcgacccgga cctcgcgtcg ggcgccggac gcctcccgcg
     2941 gggagacgaa gaggacgccg tcggccccga cgccggttcc cctgcggcag aggcgccgga
     3001 tcgtctccgc ctcgcgcggc ccgacgttgg cggctccctc gaagacgagg aagtcgttcc
     3061 ccgcgcccgc catcttccgg aacgtccgcg gcaggccgcc gatcgtctcc gcgctcatcg
     3121 ctccgccctc ccgctctcgt tcccggcggc gtcgaccgcc gtcaccgcgt agcgccgccc
     3181 cgcgggggcg ccgccgtcgt accaggccgg gtcggtcagg ccgtcggcga cccgccgcca
     3241 ctccccgtcg ccgtcccggg cccagacgcg gtacccggcg aggtcccgtg cgagagaggg
     3301 gttccagacg caccggttcc ccccttcctc ggcaaggacg agaaggccct ccgggacggc
     3361 cggcgggaag acgtcgcgcg tgtcggcgac gacctcgtcg gccgccgggc cgaggaccgg
     3421 cggcaggtcc gccgtgggcg cggcacggag cgtgtagacg taccgtttcc ccaccgcgac
     3481 ggcggtgtcg acgtacggcg agcggcccgc gacgccgagc cactcgccgt acgcctcctc
     3541 cccctcctcg cgccggtaga ccgcatagcc ggcgacggag gccggcgtcg acccgtcgag
     3601 catccccgcg ggaggaagcc actcgaggca gacccggccc tcctcgaccg tcgccgaaag
     3661 gccgacgggc tccgccggcg ggaccttcgg gacgatcgcg acgagcgggg agagcgggga
     3721 gacgcgcttg cggtcacgcg tcgccgtgac gccgtagcgg aggaagacgc gcccgagccg
     3781 cttctcgagg aagaggggga ccatcggatc gcgcacgacg agctcgctgc cgaccgtgat
     3841 ctcgtcgagg ccctcccgcg gcaggtcccg gatcgcttcg gcccgctgcc ggaagagctt
     3901 ctcctcccgc tcccgctccg ccgccccgtc cgccggctcc ggcgcccgcg ccccgggggg
     3961 ggcgggaacg agctcgcggt agatcgtcac gcgggtcagg ttcgtcagcg gggagccctg
     4021 gatcgtcttc gacgggaacg ggaagcggag gacgacgtcg ctcccctcct gcgagacccg
     4081 gagcggctcc gggcgcgcgg ggatcacctg cagcggcgct tccggggcga tcttccgccc
     4141 gcagccggcg gcgagcgccg cggcgaggag gagcaggacc gtccggccga ctctcacagg
     4201 acgaagcggg agaggtcgcg gctcgcgacg aggtccgcaa gggcgcgcgt cacggcggtc
     4261 cggtcgacga cgacgcgtgc gccggcggcg tcgggcccgg cgaagctcgc ctccgagagg
     4321 acccgctcga ggatcgtgtg gaggcgccgc gcgccgatgt tctcgaggct ccggttcgcc
     4381 tcggccgcgg ccttcgcgat ctcggcgagg ccgtcctcgg cgaagacgag ctcgatcccc
     4441 tccgtcccga ggagcgcctg cgcctgcttc gggagggagt gctccggctc cgtcaggatc
     4501 ctcacgaagt cggcttccgt cagggcgtcg agctcgacgc ggatcgggaa gcgcccctgc
     4561 agctccggga tcaggtcgga ggggcgcgca acgtggaacg ccccggccgc gacgaagagg
     4621 acgtggtcgg tcttcacgtt cccgtgcttc gtcttgaccg tcgtcccctc gacgagcggg
     4681 aggaggtcgc gctggacccc ctcgcgcgag acgtcggggc cgcccgcctt cccctcgcgc
     4741 ccggcgatct tgtcgatctc gtcgaggaag acgatccccg tttcctcggc gcggcggatc
     4801 gcctcctgcg gcacggtcgc ctcgtcggag aggacgtccg cctcgcggtc gaccaggacc
     4861 gcccgcgcct cggagaccgg cagacgcgcc ttcttccgct tcgggccgcc gaagaggttc
     4921 gggagcatcc cggggaggtc gatccccatc tcctcgaccc cctgcggcgt catgacggag
     4981 atctgcggcg tccgctcgtc gacgacctcg acctccacct cgcgcgcctc gtgcttgccg
     5041 tcgcgcagct cgcggcggag cctctccttc gcgtcggggc cggggacgaa tcccgcgggg
     5101 tcgacctgtg cggcgaggag ctccacgagc ttctcctcgg ccgcggcctg cgccttctcc
     5161 gcgaccctct cgcgccgctc gtcgcggacg aggcggatcg cggcctcgac gaggtcgcgc
     5221 gcgatcgact cgacgtcccg cccgacgtag ccgacctcgg tgaacttgga cgcctcgacc
     5281 ttcacgaacg gcgcgtcgac gagccgcgcg atccggcggg cgatctcggt cttgccgaca
     5341 cccgtcgggc cgaccatcag gatgttcttc ggggtgatct cccgggcgac ctcgggagcg
     5401 agctgctggc ggcgccaccg gtcgcggagg gcgacggcga ccgcgcgctt cgccttcgcc
     5461 tgcccgacga cgtgccggtc cagctcggtg acgatcctcc gcggggagag gtcgcgcagg
     5521 tggaggtcgc gcggtgaggc ggggcggctc acgggagcgt ctccacggag acggcgtcgt
     5581 tcgtgtagat gtcgatctcg gaggcgatct tcagcgaggc gcggacgatc tcctcggccc
     5641 ccagctccgt gtggcggaag agcgcccggg ccgcggcggt ggcgtacggg ccccccgagc
     5701 cgacggcgag gatcccttcg tcgggcgaga tgacgtcgcc cgtgccggag aggaggagcg
     5761 tcttctcccg gtcggcgacg acgaggagcg cctcgaggga ccggagcgtc ttctcggtcc
     5821 gccagtcggt cgcgagctcg atcgcggccc tctcgagctg gcccgagaac tcctcgagct
     5881 tccgctcgaa gcgggtgaag agggcgaagg cgtcggccgt cgagccggcg aagccggcga
     5941 ggaccgagcc gccggcaagg cggcggatct tcgaggcgcc gtgcttcaag accgtcgtcc
     6001 cgagcgtcac ctgcccgtcg ctcccgaggg cgacggcgcc gcccctgcgg acggcgagga
     6061 cggtcgtgtg gtggaaggcg ggcgtttcgc tcacggcggg gagtctaccc gcggcgtcag
     6121 ctcctacctc tccctcgtcg tgcaccggcc cgagacccgt gaccgccgaa gacgcccggg
     6181 gaccatcggt cggctgtcct gtcctcggga tacccggacg ccggtttgac ttcgtcttcg
     6241 cttcgcggtc cccgaacccc tccgcgcggg catttcttct caggccttcg ggtgggcctt
     6301 ccggtagacc tccatgaggc gctcgacgtc gaggtgggtg tatttctgcg tcgtcgcgag
     6361 ggaggcgtgg ccgaggagct cctggatcgt ccgcaggtcg gcgccggccg aaaggaggtg
     6421 ggtcgcgaaa gaatggcgga gggcgtgggg cgaggcgtga cgggaaacct cggcggtcga
     6481 gagcgcctgg tccaggaccc tgcggacgct ccggtccgtg agtcggccgc cgcgggcgtt
     6541 caggaagagc ggctcgcccc ggacgcgccg gccgcccagg agcgcgaggc gggccgggag
     6601 ccaggccgcg agcgcctcgg ccgccttctc gccgaacggg acgatccgct ccttgcgtcc
     6661 cttgccgagc gtccgcacct ggcgtcccgg caggtcgacg tcggggagcg agaggccgac
     6721 gagctccgag acccggaggc cggtcgcgta gaggagctcg aggagcgccc ggtcgcgccg
     6781 gccgaactcc gtgtcggccc ccggcgcctc gacgacggcc gccgcctcgg cgacggagag
     6841 cgcgaccggg aggttcttgt cggtccgagg ggtcgcgacg ccgcgcgccg ggttcccctc
     6901 ggcgagccct tcccgctgga ggaacgtgaa gaacgtccgg agcgccgaca ggcgtctcac
     6961 gatcgaggtc cgcgcctgcc cctcggagcg gagcgaggcg agccaggaac ggatggcgag
     7021 ggcgtccacc tctccgggcg aggtctcgcc tgccggcttc ccggcgaagc tcccgaggaa
     7081 cgcgaggaag cgcgcgacgt cttcgacgta ggccttccgc gtgtggggag agacggcccg
     7141 ctcgtcggcg aggtggcggt cccacgcgcc gagggcctcg gcgaggctca cgccgccccc
     7201 gccgcctccg gcgcgggccc gagcgccgcg tcgcgccagg cggcgacctc ggcgagggcc
     7261 cggcggacga tctcctcgcg ccgcttcgcc cggtcgcgga tcggcgggag cgcggagtcg
     7321 tcgatgaggc cgaacgtgac gttcatcggg ccgtagtgct cggggtcgct ctccgacacg
     7381 tgccgggaga gggcgccgag cgccgtcgaa aaggggagcg ggagcggctc gcgcccctcg
     7441 aggagctgcg cgagcgccgc gccggccatc agcccggtcg ccgccgactc gaggtatccc
     7501 tcgacccccg tcacctgccc ggcgaagagg acgcgcgggt cctccttgaa gcggaagagg
     7561 cggtcgaggt gcgccgggcc gttcaggaac gtgttccggt ggagcatgcc gtagcggacg
     7621 agctcggcgt tcgcgaagcc ggggagggtc cggaagacgc gccgctgctc gccgaccctc
     7681 agcttcgtct ggaagccgac gaggttccag tggctccggg cgaggttgtc ctggcggagc
     7741 tggaccgccg cgtacggctc gcggccggtg cgcgggtcgc gcaggccgaa cggcttcatc
     7801 ggcccgtgcc ggagcgtctc cgggccgcgc gcggccatct cctcgatcgg aaggcaccct
     7861 tcgaaatgga ccgccttctc gaagtcgtgg tacgggacgg tctcgccggc gaggagcgcg
     7921 gcgacgaacg cctcgtactc ctcgcgcgtc agcgggacgt tcaggtagtc ggcccctccg
     7981 cctttccccc agcggctcgc ggcgtagagg acggaaaggt cgagcgacga cccttcgacg
     8041 atcggggcga cggcgtcgta gaagtagagc cggtcgcccc cgagccgcgc gaggagcgac
     8101 tccgccaggg gcgccgacgt gagcgggccg gtcgccacga cgacgaaacc gtcgccgggg
     8161 tcgggaaggg ccgtcgcctc ttcgcggacg agctcgatcg ccgggtcgga ctcgacccgc
     8221 gcggtgacgg cccgggcgaa gatctcccgg tcgaccgcga gggcgtcgcc ggccggcacg
     8281 gccgcgcgcc gcgcttcctc catcacgagc gacccgacga cctccatctc gcgcttcagg
     8341 aggccgacgg cgttccccgg gttgtccgaa cggaaggagt tcgagcagac catctcggcg
     8401 aggcggtcgg tctcgtgggc gtcggtcgtc cgggcggggc gcatctcgag gagcctcacg
     8461 gcgtggccgc gccgcgcgag ctggtacgcg cactcgacgc cggcgaagcc gcctccgatc
     8521 accgtgaccc ggggtctcgt catgcggccc tcattgtagg gaaccggccg cccggccccg
     8581 ccgctacact cccgggcgtg acgttcgagc gcgcgctcta cgacgaggcc tactggcgcg
     8641 gccttcaccc gcaccactgg ttcaagaacc cgccccgaaa gtacgccgag cgggaccgcg
     8701 acgtcctccg ggtcgtcgcg ccgcgcccgt ccgatgtcgt cctcgagctc ggctccgccc
     8761 ggggcgacgt gacgttcctc ctctcccgcc acgcccggga ggtcatcggc gtcgacgccg
     8821 cgcccgaggc gatcgccctg gccgaagggg cgcgttcgcg cctcgggatc ggcaacgtgc
     8881 ggtggctcga ggccgacgtc gccgacctcg ctccggtccc cgacggcagc gtcgacgccg
     8941 tcgccgcgat cgacctcgtc gagcacgtcg acgacccgac gctcgaggcg atgctccgcg
     9001 agtgccgccg cgtcctgaaa tccggcggcc gtctcgggat ctacaccccc gaccgcgcgc
     9061 actacgtgga gcggatgaag gcgcacgact tcgtcctgaa gcagttcccg cagcacatcg
     9121 cggtccggcg cgtcgcggag taccggcgct tcctcgctgc ggccggcttc gccgtcgacc
     9181 tcgagacgtg gagcgtctcc ccgtttccgc tcgtccggtg ggtcgaacgg gcgctcgcgc
     9241 cgctcccgct cctcggaccg acgttccgct acaggatcct gattcgggcg ctcgcgccgt
     9301 aggatcgacg ggatgcccga gccgcccgac ttcgccccga acgccgccgt ctacgacgac
     9361 gcgtactacg ccgcgatgta ccgcccgcac tggttcttcc ggaacgcccg gaagtaccgg
     9421 gagcgggacg cggcgctcct gcgcgccgtg agaccccacg ccgggatgcg cctcctcgag
     9481 gtcggctcgg cccgcggcga caccgcgttc ttcttcgcgc cgctcgtcgc cgcggtcgtc
     9541 ggcgtcgacg cggccccggt cgcggtcgag gccgcgcgcg cggcggccgc cgcgcgctct
     9601 ctcgcgaacg tgaccttcga ggtcgccgac gcccgccgcc tcccgggcat tgccgacgct
     9661 tccttcgacg ccgtcctcct cgccgacttc gtcgagcacg tcacggacga ggtcctcgtc
     9721 ccgtgcctcg ccgaggcccg ccgcctcctc gtccccggcg gcgcgctcgc gatctacacg
     9781 ccgaaccgcg accactgggc ggagcggatc aaggcgggcg tcccgggcct ccagcaggag
     9841 gaccacatcg cggtccggcc ctcggccgag gtcgtccgcc tcgtggcggg ggcgggcttc
     9901 tccgtcgacg acctcttctt ctccgcaagc ccctatccgc tcttaggggc cgtcgaccgg
     9961 ctcctccccg gcgtagcgat ctgccgcttc cggaccgtcc tgcgcgcgct ccgtaccgcc
    10021 tgaagcgccc tcgggcgcgg acgccggcgc ggcacgcgcc ttgtaaggac cgatcgccga
    10081 gggctaccct cggcccatcg acgagagaaa ggacgccccg atgtcacgac ggagccttct
    10141 cctgttcctc ctcctcgcgg ccctgattcc cggcgcggac gccgccgccc agaacactgg
    10201 cggcgtcgtc ctctacgagg ggacgcgcct gaccggcagt cggatcgacc tcgatcgcga
    10261 cgtcttcgac ctcgacgaga cggcgttcag aagccgccgg gcgcggtccg tcgccgtcgc
    10321 ttccgggtgc cgcgccacgc tcttcgagct ctccggctac cgcggcgcga gcgtcgaggt
    10381 gaccggacgc atcgacgacc tctccgccac ccggctcggc gcccgagggg tctcctcggc
    10441 gcgcgtcgcc tgcacccggg acctcgtcgg ttcgagctct gcgccgtggg ccgccgggtc
    10501 gcgcggcgtg acgctcttcc gtgattccgg cttcaagggc gactcggaga ccttctcgtc
    10561 cgacgtcccc gacctctccc gcacccgcgt cggcgcacgc caggcgagct ccgtcgatgt
    10621 ccccgagggg tgcgtcgcgg tcctcttctc gggcaccggc ttcagcggcc gctccacgga
    10681 gctgcggaag tcgcacgaca acctccgtct cgcggagatc ggcaacgaca ccgtcgcttc
    10741 cctccgcgtc gactgccggg ggacgggcgg ctccacgatc gacagcgggt ggagcgccgg
    10801 cggacgcggg gtgacgctct tccgcgactc cggtttcaag ggcgactcgg aaaccttctc
    10861 gaccgacgtc cccgacctct cccgcacccg cgtcggcgca cgccaggcga gctccatcga
    10921 ggttgcggag gggtgcacgg ccgagctgtt caccgggacc ggcttcagcg gccgctccgc
    10981 cgtcttccgg gaggcgcacg acaacctccg cctgaccgac gtcggcaacg acgccgcggc
    11041 ctcgctccgc gtcgattgcc gcggcgggta cgggctggcc ggcgccggcc ggcgccccgg
    11101 ggcgtccgga cgcggggtga cgctcttccg cgactccggc ttcaagggcg actcggagac
    11161 cttctcgacc gacgtccccg acctctcccg cacccgcgtc ggcgcacgcc aggcgagctc
    11221 catcgaggtt gcggaggggt gcacggccga gctgttcacc gggaccggct tcagcggccg
    11281 ctccgccgtc ttccgggagg cgcacgacaa cctccgcctg accgacgtcg gcaacgacgc
    11341 cgcggcctcg ctccgggtcg actgcggccg gcggcgcttc cgccccgccg acggacgcgg
    11401 ggccgggtac ggctccgcgg gggtgacgct cttccgggac aaggacttcg acggggaggc
    11461 cgagtcgttc cgttcggacg tccccgacct ggcgcggacg cgcgtcggag ggcgccaggc
    11521 gagctcgatc gaggtcccgg cgggatgccg ggtgacgctc ttcagcgagc cgagctaccg
    11581 cggccgctcc gcgacgttcg acgcggacca cggcgacctc cggcagacgc cgatcgggaa
    11641 cgacgccgcg cagtcgatgc gcgtcgactg cggctcgacc cgctggtgag gcgaggagcg
    11701 cggggcgccg ccaggcgccc cgcgttcagc ccttgcgggc ccgtttcgcc gcgggcctct
    11761 tcttctccga ggcggcggcg gccgccgctg ccagggccgt cgcctcgggg accttcgtga
    11821 cgaaggccca ggcgtcgagg tcctgcgcgg gggcgtcgaa gtcgcacccc tcccgctcgc
    11881 agacgtagaa ggcgcccgcc ttccggtccc gcacgaggag atagggcgcg ccgcacgcgg
    11941 ggcaggcctt cggcaccggc cgcgcgcgga agttcctcgt gcacgtcggg tagttcgagc
    12001 aaccgtagaa cggcccgaag cgacccttgc ggacgaggac ctcgcccgtg ccgcagtcgg
    12061 ggcaggcgac gttcgtcgac gtgacgggct tcggcggccg gtagcggcag gcggggtagt
    12121 tcgagcagcc gacgtagtcg ccgaaccggc cgtgccgggc gacgaggtcg tggccgcaga
    12181 gggggcactt ctcgcctgtc ggaacgtccg gccgggcctc ggccttgccg ttcgccacct
    12241 tcacggcgac cgtgaaccgg cagttcgggt aggccgtgca gccgaggaac gcggagccgt
    12301 cccgccccgt ccggagcttc atcgggctgc cgcactcgtc gcaggtctgc ccctccagct
    12361 ccgaggcgtc caccgggtcg tcttccggct ccgggacatc gaccgtgtag tcgcacccgg
    12421 gatagccggc gcaggcgacg aagagcccgt tcttccccat ccggaggcgc agctcgtccc
    12481 cgctcttcgg gcatttctca ccgactccct cggggagggg gaagcccggg aggcgcttcc
    12541 tcacctcggc gagagcgagc cccgccttca ccgagatcat cttcttcagg gcgcggtcgc
    12601 ggtccttcgt gaagcgcgcg tcgaactcgc cgaccgcttt cctccagtcg agcttcccct
    12661 cctcgacctc gtcgagctcc tcctccatcc gggccgtcga ctcggtctgg aagaagtcgt
    12721 cgaagccctc gcgcatcagg tcgatgacga ggcgaccgag gagcgtcggg aggaagcggc
    12781 ggtccttctt cctgacgtac tcgcggtcct cgatcttccg gaggatctcg gcgtaggtgg
    12841 acggccggcc gatgccgttc tcttccagga acttcacgag cgaggcttcg ttgaagcggg
    12901 gcggcggctg cgtctcgtgg tcctcgacct gtccttccac gaggcgcagg acgtccccct
    12961 cgccgagctg cggcaggcgg accttccctt cctcggggtc gccgttcccc tctccctcgg
    13021 cctcctcccc ttcggccacc tggccgtaga cgcggagcca gcccgggtcc ttgaggaccg
    13081 agccgctcgc gcggacgccg gcgacctcgg tgctcccggg gcgcgtcgcc tcgatctcga
    13141 cggtcgtgac gtccgagacg gagggcgcca tctgcgaggc gacgaagcgg ttccagatca
    13201 gccggtagag cttcgcctcg tcggcggaga ggtcgcccgc gacgacttcc ggagggagat
    13261 ccatcatcgt cgggcggatc gcctcgtggg cgtcctgggc gtccttcttc tgcttgaacc
    13321 gccgcggctc ggaggggagc gcgtcggcgc cgtacgtcgc gccgatgtgg gcgcggaccg
    13381 ccgcgagagc ctcctcggag acgcgcgtcg agtcggtacg catgtaggtg atgaggccca
    13441 cggagccccg tcccgcgatc gtccgcccct cgtagaggcg ctgggcgacc tgcatcgtcc
    13501 gcctcacggt gaagccgagg gtccgggccg ccccctgctg gagcttcgag gtcgtgaacg
    13561 ggggaaccgg gttcgtccgc ttctcgcggg cctcgaccga cgcgatccgg aacgtggcgg
    13621 cgcgcaggtg cccgaggacc tcctcggccg cctcgcgcgt ggcgaggcgc gggtcggttc
    13681 cgtcgaactt cagcttcgcc ccgcgccagg cgaccgtccg ggccgggaag ggggcgtccc
    13741 ccttcgcgag ctgaagcggg acggagaagt agggaaccgg gacgaagcgc tcccgctcgg
    13801 cctctcgctc cacgatgatc cggagcgcga ccgtctggac ccggccggcg gagaggcccc
    13861 gccagacctt cttccagagc aggtcggaga cctcgtagcc gacgagccgg tcgatgatcc
    13921 ggcgggcctg ctgggcggcg acgcggttcg cgtcgatctc gcccgggctc gcgatcgcct
    13981 tctggatggc gctcttcgtg atctcgttga actcggcccg atggaaccgg gcgttcgggg
    14041 ccgcgggccg gaggagctcg tggaggtgcc agcagatcgc ctcgccctcg cggtcggggt
    14101 cggcggcgag gtagatcgcc tcggccccct tcgccaggcg cttcaggtcg gcgatcgtcc
    14161 gctgcttgcc agggaggacc tcgtaggtcg gctcgtagcc ggcctcccgg tcgatggaga
    14221 tccccttgcg ggggaggtcc cggacgtggc cgtagcaggc gtggacggtg aagtccttcc
    14281 cgaggaactt cccgagcgtg ccggccttgg cgggggactc gacgatgacg agagatttcg
    14341 acatgcggcc ggctacgatg ccacggttgt caagcccgcg gcgccttcct ggcgaagagc
    14401 gcccccggaa gggcctcggc gagcccctcc agctcgagga ggacgagcgc cgccgagagg
    14461 cgggccgcgg agaggccggt cacggcggcc agctcgtccg ccggccgcgg ctcgacggcg
    14521 tcgagggcga ggaggacccg cgccgcgtcg gggtccgcgg gcgtcgccgt cgcacccccg
    14581 gcctcgggcc gagccgccgg gcggagcctc gcgaggtccg cgtcggggag ctcgcggagg
    14641 acgtccgccg cgtcccggac caggatcgcc ccgtctttca ggagggcgtt cgtcccggcc
    14701 gactgctccc cgaggacggg gccagggacg gcccccacgt cacggcccgc ttccgtggcg
    14761 cagcgagccg tgatgagcga gccgctccgc ggcgccgcct ccacgacgac gacgaggcgg
    14821 gcgcagccgg cgatgatccg gtttcgggcc gggaagtgcc acggttcggg ctgggtcccg
    14881 ggcggcagct cggtcaggaa ggctcctccc gcgaagaccc gctcgcgcag gcggccgtgc
    14941 gcggccgggt agaggacgtc gaggccacag ccgagcacgc cccacgtctc gccgtcctcg
    15001 tcgagagcgg ccgcgtgagc ggccgcgtcg atcccatggg cgaaacccga gacgacgacg
    15061 actcccgctc gggcgaggcc cgcggaaagg cgacgggccg cctcgagccc ggtccgggtg
    15121 gcgcggcgcg acccgacgac gccgacgacg ggagcgttcg aaagggtccg accgcggacg
    15181 aacaggacgg gtggaggatc ggcgatctcc cgaaggagag ccgggtactc cgggtcgcag
    15241 atcgcgatcg cccggccccc gagcgcgccg agaccgtcgc gcgccgccgc gaggccgtcc
    15301 gcggacggcg ggacgaggag ctcctcgcat ccgggctcgc cgccgagcgc gaccgcctct
    15361 gccggcgaga gaccgagcgt cttctcgacg gagcccgtcg ccagaaggag gcgcgcgagg
    15421 gttcgcgcgc gcgttccccg gacccggagg ccccacgaga ggacgatgtc ggcctgcatc
    15481 cgcatggtgg gcatcttgcg taaggagcgc tgactataat gccgtcctcc gtgagacaca
    15541 gtcgccgagc ccccctcgcc aagggcctcc tcctcgcgat cgccgtgtcc ctgaccggtt
    15601 gcgcctccgc ctcgcgggac gccgccaacg ccgccgacca gaaagcctat ggggcccgga
    15661 tggccgagaa ggggttctgg cgggaggcgc tcttccgctt cgagcgggcc gcgaagctcg
    15721 ctccggacga ccccgaggtc ctgaacaacc tcgcggtcgc ctgcgaggcg acgggggaga
    15781 cggcccgcgc cctggccgcc tacaagcgcg ccctcgagct ccgccccgag gactcccgga
    15841 tcaagcgcaa ctacgcccgc ttcgccgagt actacacctc gctgcagcgc gcgtccggac
    15901 cgatggcggc cccgaaatga gacgactctc cgcgctcgca gccctcctcc tcctgctcgg
    15961 tgcgcccgcc ggtcctcttc tggccggctc gcgggaggtg aagctgcggc ttcccctccg
    16021 cccgaagctc gccgtgacgg gccgggagcg gctctccctc gcccccttcc tggtggcctc
    16081 caagctctcc gagcggaagg agtcgccgcg ggtgaaggac ctcgacctgg acctcgagtt
    16141 ccggcgctac ctcggcaagc agatcgcgcg ccggacgaag atgaacgtcg tcacgatgcc
    16201 ggccgacgtg aagctcccgt cgaccgagct gaaggcgctc ggcgaggcgt ccgcgtactg
    16261 gaaggagctc ggcacgcgcg tcggcgccga cgtcctcctc tcggggatcg tcgacttccg
    16321 ggtcgaggac aagtcgggct acaagaccca ggagtaccag agcccggtcg acggccggac
    16381 ctactaccgc caggtcttcg tcgagacgac gggattcacc ttcgaggtga ccgtcctcgc
    16441 gttcgacggc cggacggggg cgaagctctt ccaggagacg ttcaaggacc agaaggaaaa
    16501 gtcccggcgg gccgccgacg agatggtcgg gctcttcgag aacctcttct cgctcgagaa
    16561 ccagctgctc ggcctcttcg tcgtccggga aagggaggcc aaccgctatg tgttcgacta
    16621 ggcggcgcgc cgcgcggctc gtcccggcgg gggccctcgc ggccctcctc ctcgtgacgg
    16681 ccggcccggc tccggcccag ggccagaaca aggtcgtcta cgaccggttc gactggaaga
    16741 tctactcctc gacccatttc cggatctact actacgggaa gggggaggag gccctcggca
    16801 aggtcgcctc gatggccgag tcggcctacg acgacctctc gcgccggctg aactaccagg
    16861 tcccgaagcc gattccgctc ctctactacg tctcgcacgc ggagttcgag cagaacaaca
    16921 tcatcctgaa cttcatcccg gagggggtcg gagcgttcgc cgagccggcg cggaaccgga
    16981 tggtcctccc catcgacctt cccgacgaca agctccagaa cctgatcgcc cacgagctga
    17041 cccacatctt ccagtacgag atcctcttcg gcggccgcct ctcccgcgcc atcaccgcga
    17101 acatcccgac ctggttcatg gaggggatgg cctcgtactt cgcgaaggac gaggacgaga
    17161 aggaccggat gttcatccgc gacgcggtga actccgacca gatcccgccg atcacgcgga
    17221 tcaacatcac cggctacccc gcctaccgct tcggccacgc gttcttcgac ttcgtcgagg
    17281 ccgaatgggg caaggacgcc gtccgcgagc tcgtcttcga gttccgctcg ttcctcggcc
    17341 gggacgtcgc cgtcgcgctt cgccggacgt tcgacgtcga gcccgacgac ttcgacctga
    17401 agttccgccg gtacctgcgc cggaagtacc tcccgctcct cgcggtgaag ggggaggcgt
    17461 cggagtacgg cgagcggttc cgcgtcggcc cctgggagcg cccctcctac gaggtgggag
    17521 tcgccccgtc gccctcgggg gacttcgtgg cgacgctgac gacgtacaag gaggacgtcg
    17581 acatcgccct cctctccacg aagtcgcgga agctctggaa gaacctctcc tccggccgga
    17641 cgacgaagta cgagtacatc tcggcccagt tcctcaccca ggctcccgag agcggtcgcg
    17701 acctctcgtt cgcgccggac ggcgaccgga tcgccgtctt cgcgcgacgc gagcgaggcc
    17761 gccagctcta catcttctcg gcccgcggcg gagggatcct cgagcggatc cccgtctccc
    17821 ccgacatgcc cgtcggcccg tccttctccc ccgacgggcg ctccgtcgtc ttctccggga
    17881 tctccggcac ctcgcgcgac atcttccggc tcgacctcga gacgaaggcg gtcacgaacc
    17941 tgacgtcgga cgaggcgtac gacacggcgc cggtctactc ccccgacggg aagtacctct
    18001 actacacgaa gtcggtcggc gagctccaga agatcgtccg gttccccgtg gacgacccga
    18061 cgaaggtcga gcaggtcacc tggggcgagg gaaacgacga cgacgcggcg ctctcccccg
    18121 acggggagcg tctctacttc gcctcctccc gcggcggcat cttcaacgtc tacggacagg
    18181 acctgaagac gggagagatc gtccagtaca ccgacgttat cggcgcggcg ttcggccccg
    18241 ccgcctacat cgggccggac ggccaggaga aggtcgtctt ctctggctac caggcgggcc
    18301 ggatccagct ctacgtcgcc gacgcgaaga agccgttccg ccggctcgag gagaaggccc
    18361 tctcgccggc gccggtcgac cccgccgccg gtcccggctt cgtccccgcc atcgaggtgg
    18421 cgatcgacca cgagaaggtc gagaagagcc gcttcgccct cttcatcgac gacgtcggcg
    18481 cctacgccgg cgtccagtcc gacggcacgt tcgtctcctc cgcgcgggtc tccctctccg
    18541 actacatcgg cgaccggcgc gccttcgtca tctgggacac gctcgcgggg tactcgaact
    18601 tcaacgcgat ctacttcgac ctgaagcgcc gcctccagtg gggcctccag gcctacgaca
    18661 gccgcatcta cttcctcggc tacaaccaga tcggcgagct gacgtccgac cgtcgcttcc
    18721 tccgcgagac gggcgtcaac gtcctggggt cctacccctt ctctcgctac acgcgcgtcg
    18781 aggggtcgct cgggttcgtc tcgcgctcga tcgacgcccc ctacgcctac acgaacgccg
    18841 acggcacgca ggggcagatc gtcctcccgc ggaaggacaa cacgatggcg gccgggctcg
    18901 ggttcggcta cgacgcgacc cagttcaagg ccttcggccc tcacggcggg cgggccctct
    18961 ccgtcaacta ccgatacctc cccgacttcg aggacggcgg gacgctctcg caggagatca
    19021 ccgccgaggc gcgcctctac gtccccgtca cgcgccggtc gaacttcgcg atccgcgcgt
    19081 tcggcggggc cgcgtccggc gcctctccgg ccgtctacgc tttcggcggc ctcgacaacc
    19141 tccgcggcta cgactaccgc tccttcatcg gcaaccggat cgcctacctg aacgccgagt
    19201 tccgcttccc tctcatcgac gccctgatca ccgggatcgg gctgaacgtc ggcggcgtga
    19261 ggggtcgctt cttcttcgac gtcggcggcg cctggctgga gggggaggac ttcaagtggt
    19321 gggacgacgg gcggctccgc gacgggcgcg cggcgtgggg cgccggcttc accgcctatt
    19381 tcctcgggat cccctggaac gtcgacttcg cccgcccctt cgacgggcgc tcgaacctgg
    19441 cgggctggca gacgacgttc tacatcggcc cgggttcgtt ctgaggggcg cgaccgggaa
    19501 agggtgaccc ggatcacccc gcaccggtcc gccccgccct agaattcccg cgtgaaggtc
    19561 cgcgtcctcg gagcgtacgg cggctcctcg ccgtggcatc ggcagacctc gttcctcctg
    19621 gacgggacgg tcgccctcga cgcgggcgcg ctcacccagt ccctgacgct cgaggagcag
    19681 tcccgcgtcc gctcgatcct cgtgacccac tcccacctcg accacgtcgc ctcgctcccg
    19741 ttcctcgtcg agaacgtctt cgggcggacg gaggggccga tcgagatcgt cgggccgaag
    19801 gacgtgacga cgctcctgca ggggcacctc ttcaacgacg cgatctggcc cgacttcacg
    19861 cggatcccga cgcacctcct gccggccgtg acgttccgcg cggtcgagga ggggacgccg
    19921 ttccgcgtgg acggcctgca cgggctcgcg gtggcggtct cgcacgtcgt cccgacgttc
    19981 ggctacctcc tctgggacga ccgtgcctgc gtcgtcttct cgggcgacac cggtccgacc
    20041 gaggccctct gggccgcggc gcggagtcgg gacgacctga agtcgatttt cgtcgagtgc
    20101 tcgttccccg actccatggc cgagatcgcc gaggtctcca ggcacctcac gccggcgacg
    20161 ctcgcgctcg agatgcggaa gttcccggcc ggcatcccgg tgaacctcta ccacatgaaa
    20221 ccgccgacgc tgccgcagct gcgcgccgac gtcgccgccc tctccgagcc gcgggtgcgg
    20281 atcctggacg acggggacga gctggagttc tgaaaggttc gcggggggac ccgggccctg
    20341 cgggcccgcg gccggtcccc ccgcaccccc tccgcgccgg ctctgatctc cgcggcgtcg
    20401 cccgcgccgc cctccgggcc agcggccggt ccccccgcgc cccctccgcg ccggctctga
    20461 tctccgcggc gtcgcccgcg ccgccctccg ggccagcggc cggtcccccc gcgccccctc
    20521 cgcgccggct ctgatctccg cggcgtcgcc cgcgccgccc tccgggccag cggccggagc
    20581 ctcagctgcc ggccgcgacg cgatcgaggg cgaggagctc gtttgcgagc tcgaagacga
    20641 ggcggcggcg ggtcccgtcg gggaggccgc gcggcggtac cggatcgcgg agaaccccga
    20701 ggaggcggag ccgggtccca tcggttcggc gctgcaggcg aagcgtccgg gagcgaaaga
    20761 gggcgagggg cccctcggcc tcgatgacga gcccgtcggg gacgacgcgc acccgctcgg
    20821 tccggagcgt ctcctcggct tcgcgcacga cccaggaata cccgtcggcg cgcatttcga
    20881 ccgcggtcgc ggaccactcc tcgggaagcg tgcgcgcgaa cgactccggg tcgccgagga
    20941 gcgcccggaa cgcctcgggc cgcgcctccg acgcgaggta gaccgcgagc gtcggcgggt
    21001 ccgcggtcgc ccagcgttcg tcctccggcg gcgatatcgg gccgaacagc gcttcgaggc
    21061 cgtcgtcgtc gctcgccggg aacgggactc ggaggcggct ccaggcgtcc gagacctcga
    21121 ggacgccgcc cttccagcgg aggagccggt cgtcgaggag gcggaagagc cccggctcgc
    21181 gaggaggttc cttcgtcgtg tccgcggacg cggcgcccgg cccgggatcg acgctgtaga
    21241 ggagccgctc ttcgccccgg gtctcgaggt ggagccccgg cccgtccgat ccgcgccgga
    21301 cggagagcgc gccgtatccc gtcgcgagct ctcgcgccgg cggctcgagc ggaacgggag
    21361 cgggaagcgg aaacgagacg tcgaggagga gccggccgtc ctcggccagg aggatcgtcc
    21421 gcacgtcgcc ggcggcgcct cgcgcgcgtg ccggcccgat ggcgaagccc gcttcggccg
    21481 ccaacgccgc gaacgcggct gtccgggcct cgccgcagag gccggtgccg ctttcgagcc
    21541 aggccgagag gtgatcttcc gcacttccgt ccggcgcgga acggagcgcc acctgggcct
    21601 ggaagcggac gaagaggtcc ccgaggaggc gcgcctcggg gagcgctccc ggcgggtgtc
    21661 cgagcgcctc gagcgcctcg cggacggcgc tcacccgcgg tctcaggcgg cccgcgccgc
    21721 cgcgccgcca aagacgaggt cgtagacgcg cacgtcgtca gccagctccg ggtggaacgt
    21781 cgccgcgacg aggttcccgt cccggacgag gaccgggtcg ccgttcctcc gcgcgaggac
    21841 ctcgacgccc ggcccgaggt cggcgagctt cggcgcgcgg atgaagaccg cctcgagcgg
    21901 ctcgctcccg aggacggagc ggtcgaggtc ggtcagcgtc acgacggcgg agtcgagctg
    21961 ccggccgtag gcgttcctca cggccgtcgc gtcgaggagg ccgagaccgt tccggtccgg
    22021 gctcgtgatc ctccgcgcgg cgaggatcgc gcccgcgcag gtgacgaaga gggggtcgcc
    22081 gcgacgggcg acggcgacga tcgcctcggc gatgccggtc ccctccatca gcttccacat
    22141 cgtcgtcgac tccccgcccg gcaggacgag gcccgcgctc gcgcggacct cctccgccgt
    22201 ccggacgggc cgcgagtcga ccccgagccg cgcgagggcc gccccgtgcg cttcgaagtc
    22261 gccctgcagg gcgaggacgc cgatcaccct caccagcccc gggtctgcag gagctctgac
    22321 tcgacgagct tgcccgcctc gatccccttc atcccctcga cgagcttctc cgaggccgcc
    22381 gcgacgcggg ccgggtcgtc ccagtgggtc gtcgcctcaa cgatcgcgcg ggcgcggttc
    22441 gccgggtcgt ccgacttgaa gatgcccgac ccgacgaaga cggcctcggc gccgaggatc
    22501 cggcaaaggg ccgcgtcggc cggcgtcgcg acgccgcccg ccgcgaagtt cggcaccggg
    22561 agcttgccgt gctcggcgac ccagacgacg agctcgaacg gggcggcgag ctgcttggcg
    22621 gccgccatcc actcgtcgcg ggagagaagc gtgagcgact tcatctgccg cgtcaccgcg
    22681 cgcaggtgct tcaccgcgtg gacgatgtcg cccgtccccg cctcgccctt cgtccggatc
    22741 atcgcggctc cttcgccgat cctccggagc gcctcgccca ggttccggca accgcagacg
    22801 aacggcacct tgaagtcgtg cttgtagacg tggttctcct cgtcggcggg cgtgaggacc
    22861 tcgctctcgt cgatgaagtc gacctcgagg gactccagga cccgcgcctc ggcgatgtgc
    22921 ccgatccggc acttcgccat gaccgggatc gagaccgcgg cttcgatctc gcggatcttc
    22981 gagacggggg acatccgggc gactccgccg tcgcggcgga tgtcggccgg gacgcgctcg
    23041 agcgccatga cggccgaggc gccggcgtcc tcggcgatct tcgcctgctc ggcgttgacg
    23101 acgtccatga tgacgccccc cttgagcatc tcggcgagac cgaccttgac ccggaaggcg
    23161 tcggaggtga acggggtgga gaagacgggg gtgctgctca tggcgtgtcc ctttcagatc
    23221 tggatgaggg cttcctcgcg ccgctcctcg gcgcgggggg cgcggcggtg cgcccgccgg
    23281 acggccgccc gggcgaggac gccgaggctc tcgagggcct tctcgagctc ggcgcccgag
    23341 cttcgggcga cggagattcg gagggagggg acgtcggccc gatcgggggc gtagtcgccg
    23401 ccgggggaga cgaggacgcc ggcgccgcgc gccgcctcgg cgagctcgcg gccggagacg
    23461 tcctcgggca ggtcgaccca gaggacccag ccgccgagcg gtcgctcgca gcgcgtcccg
    23521 accgggaagc tgcggaggac cgtcttgtgg gcgagcgcca ggcgcgcctt cagccgcgcc
    23581 ttcacgcgga ggaggtggcg gtcgtagtcg ccgcgcgaga ggaagacgtt cagcgcggcc
    23641 tggaggaccg gcggcgacgt gagctcgccg atccgcttca gcgcggcgag gcggtggacg
    23701 aactcgcggg ggcccgagat ccagccgacg cggacgcccg gaaagagagc cttggagatc
    23761 gtcccgatgt ggacgacgtg ccccggggcg agggccgcga gcggaggtgg gagctccccc
    23821 tcgacgagga ggtccgcgtc gaacgcgtcc tcgacgacga ggacgcccgc cgccgtggcc
    23881 gccgccacga gctcgcgccg gcgcgcggcc gtcgcgagga ggccggtcgg gttctggaaa
    23941 tccgggatcg tgtagaagaa cttcggccgc tcggcgagcg cggagcggag gggctccatc
    24001 gccgggcctt ccgcgtcgag agggacgccg aggaggcgcg ccccctgcgc ccgcaggagg
    24061 gagagcgctc cggagtagct cgggctctcg atggcgacgg cgtcgccggg gtcgacgagg
    24121 gcgcggccga tcaggtcgag cccctgctgg gcgccgttca cgatgagcag cgcgtcggcg
    24181 tccgccggga cgccacgcct ctcgaggcgc gccgcgagcg tctcgcggag gggccggtag
    24241 ccgaacggcg agccgtaggc gagaagcgcg ccgtcccggg cgacgtcggc gaggaccttc
    24301 gcgaaggcct ccgccggaaa ctccttctcc tccggggcga ggcgggccag gtcgtgcgct
    24361 ccgtcgagcg gtccgtccgg cacgcccgcc tcggcgagca tccgaccgag gagcgccgag
    24421 gagatccggt cccacgacgg ggccggcccg accatcggcc cggccagcga ccggcgggcg
    24481 acgcgggtgc cgcccccctt gttcgtctcg atccacccct cgcgtttgag gacctggagc
    24541 gcctggtgga ccgtcgcccg gttcacctcg tgcgtccggg caagcgccct cgaggcggga
    24601 aggcgcgagc cttcctggaa cctcccccgc cggatgtcgc ggatgatccc gtccgcgatc
    24661 gtccggtaga gggggatgcg cccgggggcg ccgtccgcgg cggaagggcg gtccgtcacg
    24721 cccctgagga tgatccttgg cctagaccaa ggcaagatta cgagggccag agcccaacca
    24781 tgaggccgcg ccggcccggc cggtcactcc ccgcgggccg cccgttcgtc ccagagccgc
    24841 cgaagctcct cggagaggag ctggtcgaac tcccgcgagc cctcgggaac ccgacggccc
    24901 cgcgcgagga cccggcgccg ggcctcggcc gagagcccgt cgctctcctt ctcccactcc
    24961 tgcaacgccg aggcgatccg gttcctcacg aactcggctt cggccgtcag ctcgacgccc
    25021 ggggtcttcc ggagcgcatc gagcacccgg tgcgacaggt cgttggcgcg ttcgcgggac
    25081 agggccatgt ccttccttcg tcgcaggcgc gggaacgctc ccgccccgcg ttcattcccc
    25141 tcgtcggggg gtgcgggggg atcgcgaaac ccccgcgttc atgtcagtgg acgcggcgcg
    25201 gcccctcgcc cgcgtgcgcg cgaatcgccg cgacaccctg cgcggtgagc gggtggtcga
    25261 agaggcggtc gaggacaggg tacgggatcg cggccgcgtc ggctcccatg gccgccgcgt
    25321 cgagggcgtg gaccgggttc tggaccgcgt cgacgacgat ctgcgtctgg agcccgtagt
    25381 tgtcgtagat cctcacgacc gcgtcgacga cgtccatccc gacctgcccg gcgccgtcca
    25441 gcccggagac cgcgacggac gcgtaggcgg ctccggcccg ggcagccagg agggcctgga
    25501 gcggcgtcgc gaggaggtgg acgtccgccg cgatctgctc gtcgttcagg agcctcacga
    25561 cgcggagccc ctcgcgggtc atcgggagcc tcacgacgat cgccttgccg agcttcgcga
    25621 gctcgcgggc ttccttgtac atccccttcg gctcgccggc cgccacggcg gcgaagaccg
    25681 ggccgtcgca gacctgggag atctcggaga ccgccttgcg gtagtcgcgc cctgccgcga
    25741 ggaccgcgtc ggcgcggacg acggcgccgt cgagcagccc ccattccttc gcgcggcggg
    25801 cctcgtcggc gtccacggtg tcgaggaaga acctcatggc ctcactctaa ctcgctttcc
    25861 ggctcgtccc cggagggggc gaccggcgat tcggcgtcgt cgtcctcggc cgcctcgtcc
    25921 cgctcgccga gcgaggcgag cttcgcgacg gcgacgacgg tgtcgccctc gtcgaggtcg
    25981 atcaccttca ccccctgcgc cgcgcgtccg gtctcgcgga ccccggcggc ggcgaagcgg
    26041 atcatcatcc ccatccgggt gatgacgagg agctggtcgt cctcgccgac cgacttgatc
    26101 ccgacgacgg ggccgttctt gtccgtgacg cgcacgttga tgacgcccgt gccgccccgg
    26161 gtctggtggc ggtactcggc gacgtccgtc ctcttgccgt agccgagctc ggtcacggcg
    26221 aggagggtcg cctcctcgtc gacgaggtcc atctcgacga cctcgtcccc ctcgcgcagc
    26281 tcgatcccct tcacgcccgt cgtgtcgcgg cccatcggtc tcacgtcggt ctccgtgaac
    26341 ttgatcccca tcccgttccg cgtcccgagg aagacctggc gcgagccgtc cgtgacctgg
    26401 acggagagga gctcgtcctc ctcctcgagg ttgatcgcga tgatccccgc cgcgcggacg
    26461 ttgccgtagg cggagaggac ggtcttcttc acgaggccgc gcttcgtcgc gaagaggagg
    26521 aagcggtcct ccgggaatcc gcgggtcgtc gtcatcgccg cgaccgtgtc gcccggctcg
    26581 acctggatca ggttcacgag cgccttgccc cgcgccgccg ggccggccga cgggacgtcc
    26641 cagaccctca cgcagtggac ccggccgcgg tccgtgaaga cgaggaggga gtcgtgcgtc
    26701 gaggcgacga agaggtgctc gacgacgtcc tcctcggtcg cggcggcgcc cgtgcgcccc
    26761 ttgccgcctc gcttctgctc gcggtagacg gagagcggcg tgctcttggc gtagccgccc
    26821 cgcgtcaccg tcaggacgac cgcctcgtcc ttgatgaggt cctcgtcggc gaggtccctc
    26881 tcgagggtcg agatctccgt ccgccggtcg catccgaacc gggtccggat ctcggtcagc
    26941 tcggcccgga tgatcgcggc cacccgctcg ggccgggcga ggatgtcctt caggtcggcg
    27001 atgagggcga ggacgtcctt gtactcggca acgatcttct cgcgctcgag gccggtgagc
    27061 cgctggagtc gcatctcgag gatcgcctga gcctgcacgt cgtcgagccg gacgagctct
    27121 ccgtcgcgtc gcgcgcgcgg cgagaggtcg agggccggga ggtcgaggaa gcgcagcagg
    27181 gcggggaggt cgacggcgac ctcctccgta aggcgccgcc tggcctcctc ggggtccttc
    27241 gacccgcgga tgatcgcgat gacgcgatcg aggttcgaga gcgccagcgc gaggccgagg
    27301 aggatgtggg cccgtccctc ggccttcgcg aggtcgaacc gcgtcctgcg gacgacgacc
    27361 tgccggcgat ggtcgaggaa gtgccggagc atgtccttca gggggaggac gcgcggctgt
    27421 ccgtcgacga tcgcgaggaa gatgatcccg aacgagctct ggagcggcgt cagctggtag
    27481 agccggttca ggaggacgtt cgcgttctcg cccttcttca ggtcgacgac gacgcgcatc
    27541 ccgtcgcggt cgctctcgtc ccggatcgcg gaaatcccct cgagcctctt ctcgttcacg
    27601 agatcggcga tctgctcgat gaggcgcgcc ttgttgacct ggaaggggat ctcggtgatg
    27661 acgatcgact cgcggtcggc ccgcgtcgag gcctcgatct ccgcgcggcc gcggacctgg
    27721 atgatcccgc ggccgctccg gtaggcctcg acgacgccgc gccggccgag gaggatcccg
    27781 cccgtcggga aatcggggcc gggcagagcg cggaggagct cgtcgagccc cgcgtcgggc
    27841 ctgtcgagaa ggaggatcgc cgcgtcgcac gcctcgcgga ggttgtgcgg cgggatcttc
    27901 gtcgccatgc cgaccgcgat cccctcggcg ccgttcacga ggaggttcgg gaacttcgcc
    27961 ggcaggacgt tcggctccgt cagcgagccg tcgtagttcg gcgaccagtc gacggtctcc
    28021 ttgtcgatgt cctcgccgac gagctcctcc gcgagcgccg tgaggcggac ctcggtgtag
    28081 cgcatcgcgg cggcgctgtc gccgtcgacg gagccgaagt tcccctggcc gtcgacgagc
    28141 ggctcgctca tcgagaagtc ctgggccatc cgcaccgccg tgtcgtagat cgacgcgtcg
    28201 ccgtgggggt ggtacttacc catgacgtcg ccgacgatgc gggcggactt cttgtacggc
    28261 cggttgtgcc ggttgccctg ctcccacatc ccgtagagga cgcggcgatg aacgggcttc
    28321 agaccgtcgc ggacgtcggg gagggcgcgg cccacgatga ccgacatcgc gtagtcgagg
    28381 tagctgcggc ggatctcctc ctcgatggag accgggatcc gctgctcggt gggggcgggg
    28441 ggcggtgcgg ggctgtcggt catttcacgt cctcgccgcg gccgcggcct cggcgatggg
    28501 gggcacgggg ggatcgaaga aaccccgctt cattccggtc gccgggcggc ggcagccgac
    28561 cggcatgggg ggctcggggg gatcgagaaa accccgcttc gttccggtcg ccgggcggcg
    28621 gcggccgacc ggcatggggg gctcgggggg atcgagaaaa ccccgcttca tgtcagatgt
    28681 ccaggttcgt cgccgcgagg gcgttcgcct cgatgaaggc gcggcggggc tccaccgcgt
    28741 cgcccatcag gatcgtgaag agctcgtcgg cctcgacggc gtcctcgacc cggacctgca
    28801 gaagccgccg cgactccggg ttcatcgtcg tctcccagag cgtctccggg ttcatctcgc
    28861 cgaggccctt gtagcggctg acggcgagac ccttcttcgc gctctccagg atcgcctcgg
    28921 ccgcctcgac gagcgttgcg accctccgct ctccgtcgtt ccggcggagg aggtaggggc
    28981 cgtcgaggaa gccgtcgagg gcggccgcga gctccccggc gcggcggatg tcgaaggcgc
    29041 cgaggaaggc agcgtcgacg atcgtcttcc tcacgacgcc gttcacctcg agcgcgacgt
    29101 ggagcgcgaa gccgccgtgc tcctcgtcgg cgtggacctc gggcgaaagc tcggccggga
    29161 gctccgcgcc gagcgcctcc aggacgccct tctcgcgcgc cgcctcgggc cccaggccgg
    29221 ccttcacgag cgcgcggagg agctcctccg gcacgccccg gagggagagc ctcttgacga
    29281 gggagagcca ctcctgcgcc tccgtgaaga cgcggacgag ggccgctccg gaaacggaga
    29341 cgccgctcgg ctcggtcacg aggacgcggt cgtcgccgat ccgcgagagg aggaaccgcg
    29401 cgtactcctt gtcgtccttc aggaagctct cgcgcttgcc gtcgacgacc ttgtagagcg
    29461 gcggctgcgc gatgtagatg tagccgcgct cgagagccgc cctcatctgc cggaagaaga
    29521 acgtcaggag gagcgtccgg atgtgcgagc cgtcgacgtc ggcgtccgtc atcaggacga
    29581 ccttgtggta gcggatcttc tcgacgtcga actcctcgcc gatgccagcg ccgagcgcga
    29641 ggatcaggga gcggatctcc gtcgcggcga gcatcttgtc gaagcgcgcc ttctcgacgt
    29701 tcaggatctt tccgcggagc ggcaggatcg cctgcgtccg ccggtcccgc ccctgcttgg
    29761 ccgagcctcc cgccgagtcg ccctcgacga tgaagagctc ggagagggcc gggtcggcct
    29821 cctggcagtc ggcgagcttg ccggggagac ccgcgccgtc gagggcgccc ttccgcctca
    29881 cgagctcgcg ggcccggcgc gcggcctccc gcgcccgggc cgcctcgatg atcttgtcga
    29941 cgatccgccg ggcgaccttc gggttctcct cgaagtaggc cgagagcttc tcgtaggtca
    30001 ccgtctggac ccacgtcttg gcttcctggg agacaagctt ctccttcgtc tgcgaggaga
    30061 acttcgggtc gcggatcttc agcgagacga cggcggtcag cccctcccgg cagtcgtctc
    30121 ccgagaggtt cgtctccttc aggttcttcg tgaggttgtt ggcctcggcg tacttctgga
    30181 tcgcccgggt gagcgcggcc ttgagccctt cgaggtgggt cccgccgtcc ttgttggaga
    30241 tcgtgttcgt gaagcagtag atctgctcct ggtagccgtc gttccactgg agggcgacgt
    30301 cgaggcgctc gttcttcgcg tcctggaccg ggtctcccga ggggtccttc acgtcggtga
    30361 agaagatgac gttctcgtgg agccgctcgc ggttcttgtt caggtgctcg acgaaggagg
    30421 agatcccccc ctcgtagcgg aagacgtgct tcttctcgcc cgtctcgtcc cgctcgtcga
    30481 agacctcgat cgtgaccccc gggttcagga aggcgagctc gcgcatccgc tgggagagcg
    30541 cgtcgaagtt gtaggcggtg acggagaaga tctcggggtc ggcgcggaac gtgagcttcg
    30601 tccccgtctt gttcgtccgc cccacttccc ggatcggctc cagcggcttg cccttcgcgt
    30661 agggctgttg ccagacgcgc ccgccgcgcc gcacctcgac gtcgagcgtt gcggagaggg
    30721 cgttcacgac ggagaggccg acgccgtgca gtccgcccga gaccttgtag gcgttgtcgt
    30781 cgaacttccc gccggagtgg aggtcggtga ggatgatctc gagcgtctcg cggccgtgga
    30841 ccgggtggag gccgaccggg atgccgcggc cgttgtcctc gaccgtcacc gtctcgtcgg
    30901 cgtgcaccgt cacggtcacg cggtcgcagt agccgacctg cgcctcgtcg atcgcgttgt
    30961 cgacgagctc ggtcaccatg tggtggaggc ccgtgatgtc gtcggtgttc ccgatgtaca
    31021 tcccggggcg cttgcgaacc gcctcgagcc ccttcaggag cttgatcgac tcggtcccgt
    31081 agctgctttc gcgtccctcg agggagacgt cgtccggttc aggaggcgag gacatcatcg
    31141 gacctttccc ccggccaccg ggccggggcg ggaacgaacg ccgccggctc ggcggcgggc
    31201 ccggacgggg cgaggcggcc ggcctcgacg cgccagacgg cgcctgccgg cagggggagc
    31261 tctcgcgcga cgcgctcgcg cgcggaggtg aggaggacct gtccgtcgtc gggaagcgcc
    31321 cgcgcgaagc gggtgaggac gtccgggtcc cactcggagt cgaagtcgtc gaaggcgaag
    31381 acggggaggc gtccgccggc ttcggcgagg aggccccgct ccgcgagcgc ccaggcgagg
    31441 acgagggcgc gcgcctctcc cgaagaggcg cgcggggcga gcgggcgctc ttcgaagagg
    31501 aggacgaggt cgtcgcggtg gggcccgaag agcgctctcc ccgcgcgccg ctcactctcc
    31561 tcgcgttgcc ggagcccgga gcggagcgcg tcggcgagca gcgggagaga cccgtgggcg
    31621 ggtagctccg agacgagccg gaacgagatc tcccggtacg ggaggccgag gagccgggcg
    31681 ttctcctcga ctcgtcgccc gagcgggccg acccagcgcc tccgggaggc cgcgatcgcg
    31741 gctccggcgg aggcgaggat ctcctcgaac gcggcgaggg cgtcccggtc gggccggccg
    31801 ggagcggcga gaagcgcgac gcggtcggcg cgggccttct cgtagcggcg gtgctcggcg
    31861 gcgagggcgg gctcatgggc gacggccacg cggtcgagcg cgcgccggcg atccgagggc
    31921 gggcccccga ggcgccccag gtcggcggcg gtcaggacga cgagagggag ccttcggctc
    31981 gcctcgacgc gagagacctt cctgccgttc cagaagtgct cgcggcgtcc cttcccgatt
    32041 cgggctgcga gctcgccggc ggattcgccc acgagaccga cggtcaccgt cgcgtcgttc
    32101 gctccctcgc ggacgacgtc tgcgaggtcg gcggtccgga aggacgctcg cccggacacg
    32161 agagcgaggc tctcgagcac gttcgtcttc ccctgaccgt tggcaccgac gaggaggttc
    32221 agtcgcgggt ggaatccgag acgggcgggc gcgaggttac gaaaggactc gaccgagagg
    32281 gttccgatca ccacgtgtcg acgcgcaccc cctcagacgc gcatcggcat gacgacgtag
    32341 agatagctct tcagcccggg cacgggctcc gcgggccgtc cctggcactg cgacccctcg
    32401 tccttgacga acaggtggac cttctcggtc tcgcacgcct cgaggaactg ctcgagatag
    32461 cgcgagttca gccggagggt gacggggtcg gcctcgtact cggcgccgat ctcctggtcg
    32521 ccgtcgccga cgtcctggct ctcggaggag acgacgaggg cgccttcccg gaaggagagg
    32581 ctgaccgcct tcagccggtc gccggagagg agggagatcc ggcggaccgc gccgagcagc
    32641 tcctcccgca cgaccgtcgc ccgcttcgtg ttctccttgc tgatgacctt ctcgtagtcg
    32701 gggaagcgcc ggtcgtccag cttggagaag attcgccggc gcccgagcgt gaacccgatg
    32761 tggttcgtcc cgcggacgat cgtgacgggc gtctcgtcct cggcatcgag acgcgagagc
    32821 tcgtcgagga ctttcttcga cacgaggatc gcctcgaatc cggcttcggc ctgggcgtcc
    32881 tccacctcga tcagggcgag gcggtgcccg tcggtggcgg ccatctcgag accgcccccc
    32941 ttcggcttca gcagagctcc ctgaagctgg aggcggatct cctccgtcga gacggcgaac
    33001 cggaccttct cgaccatgcg gcggagagcg ccgaacgtga gggacagggt cggcccgccg
    33061 gtcgcctccg gacgggtcgg aaagtcggtc gcggggagcc cgttgaggcg gatccggctc
    33121 ttcggcgcct tgcagtcgat ctggacgtga tggttctcca ggacccggag gcgaacctct
    33181 gccgctgcgg gaaggtctcg cacgacgtcg aagagcttcc gggccggtac ggtgacggcg
    33241 cccggaacgg agagagagag aggctctatc tcggacacga aggtcgtgtc gaggtccgtc
    33301 gccgtcagag tgagcatccc gtcacgggcc tcgaggagga cgttcgacag gatctggtgg
    33361 ggcgttcgcc gttccaccac tccctgcatc aggctcatct ctcgggccag ggttgctgcc
    33421 ggaagtgtca gctccacatc ccctccactg attccttaaa ttctagcacc gaaagagtta
    33481 cagaagaagt agtaggtggg gccgccacct gtgggaaggc ggcggaagag gagaaaggaa
    33541 cggggcttat cgcgccgcaa ggggtgtgga gatctcgacg ggggcctgtg cgtttccgcg
    33601 agacgaacac gggaggacga aatgggccgg acggaatgca cagggagtcc ccgcgagttt
    33661 caccggaatt gcgcgacgag gacggcgact tcccgatcga aatcgggatc ctcctcccgg
    33721 cgggcggaga tcttcttgat ggagtgaagg gccgtcgtgt ggtgcttgtc tccgaagagg
    33781 cggccgatct cgggaaggga caggccgatc gcctccttga gaacgtacat cgcgacctgg
    33841 cgcgggaaga cgatcttctc ccgcttcgtc cgggcccgga gctcggcggg cttcaggccg
    33901 tagtgggcgg cgacggcctt gaggatctcg gcgggcgacg tcttcgagga gtcgtcgggg
    33961 aggattcccc gcagggcctc ctccgcgagg tctcgggtga tcggctctcc ccgcatggag
    34021 gagacgagga cgacccggtt gaagtagctc tcgagctcgc ggacgtgagt cttgatcttc
    34081 cccgagaggt agtaggcggt gtcgtcgtcc agctccacgc gggcgatctc ggccttgtga
    34141 cggaggatcg cgaccttcgt ctcgaagtcg ggctgctgga tgtcgacgac cagcccccct
    34201 tcgaaccggc tcttcatccg atcggtcagg cccgggatgt cgcgcgggag gacgtccgag
    34261 gtgaagacga tctggctccc gatggaatag agcgcgttga acgtctggaa gagctcgtct
    34321 tcggtcgtcg ccttcccggc gaggaactgg acgtcgtcga acaggaggac gtcggcgttg
    34381 aggaggcgct gccggacctg gtccatccgg ttgaagcgga tgccggtgac gacctccttg
    34441 acgaaggtct cggagtagac gtagaggacc cgctcgccgg gacgccggga gaggcggcga
    34501 tggccgatcg cctgcatcag gtgggtcttg ccgacgccgg tggggccgca gatgaagagg
    34561 gggttgtacc ggtgggaggt ctggtcggcg acgtggagcg ccgccgcgtg cgcgagctgg
    34621 ttcggctttc cgatgacgaa gtgctcgaac gtgtaggcgg ggttcaggtc gccgacgcgc
    34681 gagcggttcg gctgggggcg ggccggggag acgacggccc gcgcgtcgtc cctcccttcg
    34741 aggacgaagc ggatctcgcg ggtgccgagg ccggcgaggc gggcggcctc ggcgatctgg
    34801 tccccgtact cacgcgggat gtagtcgatg aagagcgggc tggagatcaa gacgaagacg
    34861 gcatcctgcg tctcgccctt ctgcctcgtg ccgcggaacc aggtgtcgaa gtcgcgctcg
    34921 tcgacccggc tcttcaggtg atcgaggatc ttttcccagg aagtaggttt ttccacgacg
    34981 actgcaccga cgcgaaggag accgcccggc aggagcgggg gcggatgcta gcacgcggcg
    35041 cccggtcggc tgacggcgcg agccgggagc ggtgtcatgg ctccttagaa atgtcacccc
    35101 ctggaactcg atagaaatgt ccgttt
//
LOCUS       DAOONL010000030        33468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1087_length_33468_cov_14.816090, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000030 DAOONL010000000
VERSION     DAOONL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 33468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33468
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1087_length_33468_cov_14.816090"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLP50_06755"
     CDS             complement(<1..685)
                     /locus_tag="PLP50_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HPA51285.1"
                     /translation="MSIRTRVGVVFGGPSREHGVSFLSARCLLENLPSDRYEAVPVFV
                     GKDGRWHGVADSREEMLRYFARPARDALLDGVVPGSRPAPPSEIVAAFEAAFRDGGWD
                     VVFPILHGTFGEDGVLQGLLEAMNVPYVGFGVAASAVGMDKLLMKATFGAAGLPQVRY
                     VGVRAAAWRDESRRAALLARAAALGLPLFVKPACAGSSVGITKVKERAGLEAAVDAAL
                     AVDATALVEA"
     gene            complement(832..1959)
                     /locus_tag="PLP50_06760"
     CDS             complement(832..1959)
                     /locus_tag="PLP50_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPA51286.1"
                     /translation="MSSSAFDSTSGHAGQVRFFNPGPVWVRPQVLQALGGPMMSHRSA
                     AFSDLYGRILENLPKVFRTKGQAHTLATSATGVWEAGLVSCATGPVLACCNGSFSDKW
                     GEMSQRLGLETDFVRSDWGKPILADDVKKALAAKKYQVVTVVHNETSVGTISDLAAIA
                     KVVHENSDALLFADVVTSLAATRVETDEWGLDLAVCGSQKALALPPGIAVFAVSEKAL
                     AAARAKKCRGLYLDLLDVEAFGKKKQNPTTPSLPLLYALDVQLDHILAEGMEARWARH
                     HEMLKAVEEWAPKAGFRFLPDPPAHSPSVSSLFPPEGVGAEDLNKAMKGAGWVPGSGY
                     GKFKSLNIRIGHMGDVDVASVKNVLAAYEEQAAKLLSASKG"
     gene            complement(2052..5468)
                     /locus_tag="PLP50_06765"
     CDS             complement(2052..5468)
                     /locus_tag="PLP50_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51287.1"
                     /translation="MARTPLRVCRAAILVLGSLLALSSAADLVWKVADEGPRAPGSVG
                     IVGDDARVLASGSGGTWELDGTVWRRISLDASASPDGSLAPSFVAGGRFVALKLRSGG
                     IEVFVLRENAWVRLGVVPDAGQDYVPSDDRLYVSTLRFNDCEPDPAGVHDVEGRRLRS
                     ISFADGSIREEPSLPARNGGLFVLFGKLHLLEWGGAVDGCAAPSSKAGSLAAAAEAPI
                     PFHRLDGDHWTTLEPWPANGWGFRSSPNSLWGFYSVGPTGIEARVLTAAGLSAPIPDP
                     QGVYDVSYGVRPFEWNGRLLASSREARGNIYELKEGRLERLTPESPVQGERSLAPSVA
                     VAGRCLFTWAQGWDVHVREAETWVPTAGAGETIGADTYFASPTRRFATVGNRVFRRGE
                     TGWSRLAPLPAKGSITGAVAVGERAVVAVDDGTDHLLEHDPATDTWRDLKLPEGFGLF
                     DASLWNGLQRSLAATADAVWVNGTGNRLARCRDGVWTIFTPPQAPTPGSSGSVVRVLD
                     GVPHLFGWWGETRPSAWVLRNERLEEAFPGLDPGLRVLDAASAGGRTFLAVRGAYEDG
                     TAAALVSFDGAVFRTLLTLNDLRSANLSPGATAPIAAVGDTAFFAQLAFSGGRLRDLM
                     GPLAPRAIAPSGGFGWDGARRYEGEHPLLVPVPRVRKHLAAVVDSTGVGGTRYRSSLS
                     VANLSPSRSAVARLFPGTAVVPARELALRPGAQVRIEDPFPGFIGPVTVEFEGLEDES
                     EAWAAVRVWSPSESGTAGTALVATDPGGVGADGTALRPVRTPGSRLHVAMAATNDGTG
                     AGLWVWDPRSAVPWEETALAKVPRGGFLQVDPHPEAVERPLVLAPSPIAGSTGGTDEP
                     MDLLGYLVRNDGATNDGTVVPFAPPDAIPGRRTRFLPAVVGVTSAHAAYRTELTLGSR
                     STDGRRVPRLEFQASYRDDAVSKTFPVVLEAGSTPYAMLFGEVLEVPDAGAWLVANGV
                     PIDPQAFIGTLSFTAERPEGAAALLVTAVVQARAPGAKGDYGVSVPLVSEGRWARERA
                     IVPGLREDGAFRSNLALANPEADGGPEVTLEVTLHAAADGAVIGTLPRMTLAPGRRAQ
                     PDRPLGQVGYGGDAWAEVRRVGGEGRFVAYGVVNDAVTSDGTLIPMEGAK"
     gene            complement(5591..6244)
                     /locus_tag="PLP50_06770"
     CDS             complement(5591..6244)
                     /locus_tag="PLP50_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPA51288.1"
                     /translation="MTYRAAVFDLDGTLLDSYVGIHDALSTVLEHFDRPPVTVEEARR
                     LVGHGLEALIAKVLPEELRAEGAERFRFRYKVNAPKLTRLMPDAELVVSELWRRGVKL
                     SIASNKPAVFSRQLLAGFGLEELFAFVGGPDLGFPEKPDPSMVLSALDAMGVPPAGTL
                     FVGDMVVDVATARAAGLSVAVVPLGSSTREELLAQRPDHLLEALSEVLGLFEEVRAH"
     gene            complement(6241..7197)
                     /locus_tag="PLP50_06775"
     CDS             complement(6241..7197)
                     /locus_tag="PLP50_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HPA51289.1"
                     /translation="MKAILVPSPGGPEALVPAEAPEPALRDGEVLVRVRATAVNRADL
                     LQAAGKYPPPPGESEILGLEAAGLVEGTGERVFCLLPGGGYAEKVAVPRSMLMPIPDR
                     LSFEEAAAVPEAWFTAYLNLFREGGLAAGEVVLLHAAASGVGTAAIPLAKDAGARVVA
                     TVRSAAKAGPLEALGADLVVDTTARDFVEAVEECFGKAAVDLVLDPVGGSALAGNVRL
                     LARGGRLVLIATMGGGAAELDLRAVLTKRLRIVGSTLRARPLAEKADLTAAFVRNVLP
                     GFADGRFRPLVDSVFPLERAADAHRRMASNANVGKIVLTVVS"
     gene            complement(7194..8891)
                     /gene="mobB"
                     /locus_tag="PLP50_06780"
     CDS             complement(7194..8891)
                     /gene="mobB"
                     /locus_tag="PLP50_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPA51290.1"
                     /translation="MTRPRLLSGLDVLLADPTPLRGLKLGLVANPASVTSRFVPTARA
                     LVEAGLDLRLLFGPEHGLTGAVQDMIAVGADDTPSGRLPVVSLYGDRFEDLSPKPEHL
                     AGLDAVVCDLPDVGSRYYTFVWTTFLVMKACAAAGKPVFVLDRPNPLGGFAVEGNLPE
                     DRLLSFVGLRPVPARHGMTPGELARWANVESALACDLTVVPMRVDGRPAPRGRVGETP
                     DWVLPSPNMPTRDTALVYPGMCLLEGANLSEARGTTRPFETVGAPWLDPDAAADRANA
                     LGLPGVLFRPHVFRPTFHKFAGQDCGGVQLHVTDTEAFRPYETGLRLVKLLRDLDPSR
                     FRWRTETYEYRSDVPAVDLLAGTPRYRELVDAGESLDAWIATFPGDVARFAPSREAAL
                     LYRAGPPRVQVVGAQDSGKTTHALGLVEALVARGFSVGAVKHTRHEHETDVEGSDSQR
                     HFAAGASPVVFVSGRRTGVHARHDADPSLDAVVAREIPDVDVVVVEGFREAPGPKVEV
                     CRAATGREPVAADDPEVLAVMTDRETTHASSVPRLPLGEVDRLVPLVLDALGLGGPR"
     gene            complement(8888..9835)
                     /locus_tag="PLP50_06785"
     CDS             complement(8888..9835)
                     /locus_tag="PLP50_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPA51291.1"
                     /translation="MKLARPAAFSALLLLAAAAALRPARAASRIPVDVAEETTRRVLR
                     LATGAVETVPLEAYVAAVLPDEIGSFAPPAALEAQAVAARSYAVAQKGRHADQGADVC
                     DSVHCQVFRGLGRATEATRRAAAATRGLVLVQGGHVVAAPFHAVCGGRTARPSAIWDD
                     EETPELTPVEDDACLASPGARWTFFLPRAKVPLLAEKLGFPAARFLEVWAHADDGRVA
                     ALRLAAPGGRSRVVRAFDVRARASELWGWSSIRSTDFEVEERPAGYAFTGRGTGHGAG
                     LCQAGAIARARRGESRDAILAHYYRGAAVATLGARGAAE"
     gene            complement(9859..10377)
                     /locus_tag="PLP50_06790"
     CDS             complement(9859..10377)
                     /locus_tag="PLP50_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPA51292.1"
                     /translation="MRSVPLRVAVLTVSDRSARGEREDRGGPAVAEAVRRLLGAEVVE
                     TQVVPDEKDDIVWHLTRFADNVLADLVLTTGGTGISERDVTPEATRSVISLELPGLPE
                     AMRAASRSALPSAMLSRQVAGIRGKTLVVNLPGSPRGAVECLEAIAAALPHAVAMLRG
                     EGAESHPTSLRA"
     gene            complement(10549..12396)
                     /locus_tag="PLP50_06795"
     CDS             complement(10549..12396)
                     /locus_tag="PLP50_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA51293.1"
                     /translation="MAARIVPDGAALSVGGSVRRVRAPATTAVFAEPAPGGRSTDAHG
                     LGEGPLRPAGASTALRTAWRRPFPDGLVVALLALLALGLSLLDARRDSATADEPIHAA
                     AGVSQVTSGTWLVNVEHPPLAKQLVGISAVGLGGAEPPRLDFRDFFRSTRSWLFASRD
                     DSVLFASRVSVALLFAALVVGAWAAAGKGPGGIAASALLVGNTAVFPHGHLVTTDVPL
                     ALFTVLLAGSLRRFDEARRLRPALAASVWLAAALATKYSAILLLPLTVLALLARRDGR
                     PTRRDLLAAAGVPVAAILILAAALSWSVRGEEPEMIRVLGRVYRMPKEDLALLERADS
                     VHRGLARWGFGLLFHLRQAEAGRLTYFDGPTSHPGAHYHVVALVVKSPVVWLAGVLAG
                     AYLAARGGPRFARLSYVAALLLFAGSLPGPRIGVRHVFPSIVFLALGAGAALGPSLSR
                     LPRVSRAALLLVALSPLASERNLGTDGLVGRLVGRPVLADSNLDWGQDLLRLRDLLAR
                     RGIPSSQVGIAYFGGDDPSLRIPGVADLLESSAPPRRYLAVSRHLLLLGPEAVLVTDT
                     SGRAARSLALARAPGSRLVARAGESLELFDLGGTGGSGAGAAPSARRAP"
     gene            12429..12842
                     /locus_tag="PLP50_06800"
     CDS             12429..12842
                     /locus_tag="PLP50_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51294.1"
                     /translation="MEPDTVHALRARRAVAALLVGTGLLTLSAGAVRHDLSWPDSLRM
                     ARRFWRAGPRGRLLNAPGIARDAGFAAEVIRADAIWPRDEDVVLTVGRELAPERRERV
                     RRTTAYLLAPRRVLLEVGQGTELHLRPVSAKGPAR"
     gene            12839..13891
                     /locus_tag="PLP50_06805"
     CDS             12839..13891
                     /locus_tag="PLP50_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51295.1"
                     /translation="MRRLPAAAAGLLLLLGLTSATFIAVRQPLSLGDYLAIWGLKARA
                     IHFSGELDSVFRVDPEGDYSHPAYPPLWPLLLSGASRLIGRYDELLLSLVRPVLLVFG
                     AALTFARTRGTTTWRLVAAAAFTLLPYFQSAAYTGYAETLLVVLVLGALLFLERSAPG
                     CRASTAAGALLALAALTKQEGIVAVLVVAAGLLLARRRGDALRVATLGLGLGVLPWSL
                     YRAAHSVRALSVHGASAFDLEKPLRALSTLATEVGWPGLFWSAGAVALLVVGPVARRD
                     RELLLAGTLAYAALLTASFVFARIDVSWYVAWSWDRLALLPVAALLPALAEAAAEPFR
                     TGEDVAAEGSGAAARP"
     gene            complement(13868..14389)
                     /gene="moaC"
                     /locus_tag="PLP50_06810"
     CDS             complement(13868..14389)
                     /gene="moaC"
                     /locus_tag="PLP50_06810"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPA51296.1"
                     /translation="MHSQKKPDLTHLDSEGAARMVDVSGKSETVRSATARAVITLGAA
                     AWSALDASENRKGDALAVARIAGIQAAKRASDWIPLCHPLLFDAVEVRLERRRSRRQV
                     VVFATVRGSGRTGYEMEAMVAAAAAALTVYDMCKAAEKGIVVGPIELVAKSGGKSGDW
                     RRASAPSGARRRA"
     gene            complement(14367..14954)
                     /locus_tag="PLP50_06815"
     CDS             complement(14367..14954)
                     /locus_tag="PLP50_06815"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPA51297.1"
                     /translation="MAHGFVLVGGLSSRMGRDKALLPIGGVPMALLQARKLGTICTRV
                     SFVGKEPGSLAALGHPFVADGTRERAAVHGVVAALAASPVEECLVLAADLPLVPAELL
                     EALRARRDAVGAAAVAPWDGRGPQALCSAWSTRALPALRASVAGGDLSLRRALESAGA
                     LVLSAEETERLPGFRPGVFLNVNSPEDLRALAEEA"
     gene            complement(14996..16198)
                     /locus_tag="PLP50_06820"
     CDS             complement(14996..16198)
                     /locus_tag="PLP50_06820"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPA51298.1"
                     /translation="MGFVTVPAAAEHYRKGRFVIIADDEDRENEGDLCLAAEHVTAEG
                     INFMAREGRGLVCVSLTEERCDELELAPMVEQNTSNHGTAFTVSVEARGKTTTGISAA
                     DRAQTVRTIVDPRTKPHDLLRPGHMFPLRARKGGVLKRAGHTEASIDLARIAGLSPAA
                     VICEILNDDGAMARVPDLLRFSEKHGIPLVSVADIIRHRMHHERLVQRVASPSLPTRH
                     GEFRVHAYRSDLTNEEHLALVMGDIAEEEPILVRVHSACLTGDVLGSMRCDCGEQLNK
                     ALEMIAREGRGILLYLLQEGRGIGLLNKLRAYDLQDGGLDTVAANEALGFAPDVRDYG
                     IGCQILRDLGVKKMRLLTNNPSKYVALDGFGLEIVERVSLEIPPTDATRGYLSVKKAK
                     MGHLLKLV"
     gene            complement(16234..17607)
                     /locus_tag="PLP50_06825"
     CDS             complement(16234..17607)
                     /locus_tag="PLP50_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51299.1"
                     /translation="MPRFTVLETSALFSNRVKYDYLFEMEMWLKGVERFFVLDHLPLS
                     AFERSHASLKNFVEEVAVVRSGISHLALLTTHLLGEGKEDLASFLLFLDSQVSRASGG
                     APVPQRRGSAETELALVVEKLDDFSKVLDELSRAPFLPLQTYLSLGRIVVGTLKSEPN
                     LGVLFRENLRPVLDRESKQSLARVLAGVSDASQKSALAALFVELFKGLRWTERIRQTI
                     ESPATVKRALLVFSLIRAQAASLGEFIRKRLLPRFPEDTPVAAGLERLVFSVEMEMRK
                     VMELELVDVALMRDPKLVYARMEDAAGILKDLFQQSIIGLAETFSSDVDGRALFPEYR
                     TKLDQSLRLRDDLWELLQAVQAFSRGADRKALKAMIDALDAFRRGSMKHLMFKDWSLF
                     DRFHAGFSRERSPRAFLPAAHQFEVFLKTLVREVSKRSVLSSHPFRGGRPRTGDDAEG
                     AAPPRYN"
     gene            complement(17616..19679)
                     /locus_tag="PLP50_06830"
     CDS             complement(17616..19679)
                     /locus_tag="PLP50_06830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPA51300.1"
                     /translation="MALRGGARRVGAASLPILALVLTACSSVAPDADRHEARPERLAL
                     VARLLEMEDRRAWDDAPGQAAAASPDARLRERAALAAGRLRATGAVPLLSTLLADLEP
                     RVRRAAAFASGLAGERRLVPLLRSALSDADPGTAAAAASALGRLGGDAAEEALRGVLR
                     ARSGPVAAAAAALYRSKDPGLVPLLAAAAASPDGETRRMAAWSLARVPRPGSESALRA
                     LLRAGDPEVVAWAARGLGLLGDAEAAGPLSEVALGPEPGPAIQALLALERLAAGGLAR
                     DVAREAGLARRGDPHPGVSLAALTLLRAAADDADVRALLERVVVHGGRRGGVALASLA
                     AGDAERAHALAFPGKGTAPLDLRLGAAEALPLLAAERVGPWLEALLGDSAPRVRMEAV
                     SRLPRPLVPRSLPLLTRALADLDGAVRAAALDATAPFAAGTGSDARLAAAWRAAFDAL
                     VASGEADLAATALDAAASLPAGGRELLAAKRDAADDLVRERARRLLRERFGVDSTDPS
                     PAVATRLAAADTLRLAERAEGPPVRVVVETSRGSFEAELFADAAPMTVESFVSLARAG
                     FFDGTTIHRVVPDFVVQAGDPRGDGTGGPGYAIRDELNPIPYVRGRLGMALSGPDTGG
                     SQWFVALSRQPHLDAAYTVFGEVTAGMEVVDRVEQNDRLLSVRVREEPGPGEDPSAGF
                     PRGVN"
     gene            complement(19688..20446)
                     /locus_tag="PLP50_06835"
     CDS             complement(19688..20446)
                     /locus_tag="PLP50_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPA51301.1"
                     /translation="MRYLLLSDLHSNDEALSAVLARVRRKKLDRVVCLGDFVGYAADP
                     NKVLDKIRTLPRPAVSIRGNHDKVVSGVDSGEMFNAPALFAARWTAEKLSKENLEFLR
                     RLPCGPVLVDETFAICHGSPLDEDAYIFSDWDAQMNFVHMDRVAPSVAVSFFGHSHIP
                     SIFTLAPDGILVEAVRGSRRKLFLEPGRRYLVNPGSVGQPRDRNPRASYAIFDSEERT
                     VTFDRVPYDVKSARQKVLRAGLPTILGDRLLLGL"
     gene            complement(20451..20975)
                     /locus_tag="PLP50_06840"
     CDS             complement(20451..20975)
                     /locus_tag="PLP50_06840"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPA51302.1"
                     /translation="MKAGPGRVFLVGFMGAGKSTVGRALAGRLGVPFLDLDEAFESLA
                     GVTIRRAFEERGEAWFREREAELLRGCEALPDVVVALGGGTFTFPENRAVVRRAGRSV
                     FLDVPFEAIATRLEGKAADRPLFRSIEEARALYDERRASYRLSDTILAVEGDEGVDAV
                     VDLIVEALASRGVG"
     gene            complement(20989..22359)
                     /gene="accC"
                     /locus_tag="PLP50_06845"
     CDS             complement(20989..22359)
                     /gene="accC"
                     /locus_tag="PLP50_06845"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPA51303.1"
                     /translation="MFRKVLVANRGEIALRIIAACKELGIATVAVHSTVDRDALHVEA
                     ADESVCIGPAAPSASYLNITSLVAAAEITGADAVHPGYGFLAENAHFAEILEEVGLTW
                     IGPPPEAIRRMGDKSTAKDTAKRAGVPTIPGSPGPVGSTEEALKLAERIGYPVLLKAV
                     AGGGGRGMRVARNGKELAAAFELATVEAERAFSNGSLYLEKYLAEPRHVEVQVFGDRL
                     GKIVHLGERECSLQRRHQKVFEESPSPAVSPELRERMGAAAIAAARAVDYVGAGTVEF
                     LLDQDGSFYFMEMNTRIQVEHPVTEMVTGFDLVKEQIRVAAGEPLSFPRVGGATDEFR
                     PRGHAIEFRINAEDPEKFTPSPGTITTLHVPGGPGVRVDTAAYIGWRIPPHYDSLLAK
                     LIVHGQTREEALARGRRALELFVVEGVRTTIPLHLRLLDVPDVRHGRFSTKWLERWLS
                     GETPAA"
     gene            complement(22359..22841)
                     /gene="accB"
                     /locus_tag="PLP50_06850"
     CDS             complement(22359..22841)
                     /gene="accB"
                     /locus_tag="PLP50_06850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPA51304.1"
                     /translation="MNVKEIRELADLVARRGLASLEVEEGGFRLRIEGPRPAVREAAP
                     VPVSAYPAVPVSWEEKAPRIEAPAPAPPAAAPEVDSGVHVVTSPIVGTFYRAPNPEAE
                     PFVKVGDTVEKGATLCIIEAMKLMNEIEADVAGTVVEAFPKNGQPVEFGEKLFGIRPA
                     "
     gene            complement(22888..24360)
                     /locus_tag="PLP50_06855"
     CDS             complement(22888..24360)
                     /locus_tag="PLP50_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA51305.1"
                     /translation="MTRETGPRQFLVVADEHRRQGRPREAIEVLEAGLQQSPASVAGH
                     VALGRLLLQTEQLEKAVDSFQTALTLDPQNLVAIRQLAEVHLARGEKVEAVKKLKLFR
                     GLSPGDREVAELIRQLDAELAPPPSPRSGIRLPVPVPPVPPVPAPATETSVPPEARLT
                     LVDAPAAPIAPAAASVELPPTPVAEPGPGLDAAAAAEASEAESPGTPPFAEVESESTR
                     LELLPEVPAVPPSEEIPDILVESSTAPDGESPEADMGPGPFGEGARAEVLAELSPSGP
                     PASAGALEEPLAAPEAASLPIPVATETMAGLLRAQGHLGRAREAYLALARTETDPAKV
                     SRYLEVAAELAATASSSVRGRLEAWVEPFARDGRSGNGDLVTAVEEIVERLAPAAALV
                     TDLEGVPVVTVGSRLEAEAMESLSAELTAFWKNVRRARADVGEGALRSLVLKGSAGTA
                     AMQAITTEYALLLKTGAGVPVGRIRFEAARAAEQLRPALL"
     gene            complement(24510..27464)
                     /locus_tag="PLP50_06860"
     CDS             complement(24510..27464)
                     /locus_tag="PLP50_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPA51306.1"
                     /translation="MRRTLQPLFAATVGLSLLLAGCSGESPTSPKPPAGGGTDGGSCA
                     VTIALDATDVTPMAGTAIILRATVRKGGALVPDGTSITFTTDFGFFLETGLPSVSKVT
                     QNGFADVTLGATSSGLAKVKAAFECGTAEKSVDYQPIPSNGPFISSISPTSGSCAGGD
                     VVTINGGRFGTDVANVQVLFGGKPASVQSLSETRIEVLTPARTLANPLVPEVVDLVVR
                     FFSGDVPLGDITEQKAFTYYCVDPNKRLTLTSVDPNSGKPEGGQQVVITGTNFLPSPT
                     SAVATTRVTFGSAPASVVSVTGTQITVQTPRHVLANPAVPETVDVAVTADLGLVSQQS
                     GLLPQAFTYRADGGAGTCNGVPGLFLATVLPEDPTNAGSPDGGDIVVIQGGGFTAGGT
                     STTTDRTAVFFGGNQGVTLSVSDSEIRVTTPRRVLASPDKPETVDVRVIVDAGGPREA
                     CAQAVKAYTYFPGGFLEPVVTSISPSTGPNDASTRVTIFGRNFKLPMQVFVGGVEASV
                     VEIKASQIIFLTPLATGPNSALAGRTVDVVVRDPYAGKDYTSPVQFRYYACPTLNTVI
                     PSSAPWNQSTVVTIAGQGFEEPVEVTFESGGLLLRPQVTSVSSSLITLVMPVIDPQLG
                     GGPACDNVTGTLTISFPALSSAACNTLGTPFTYRVDAMTAVSATPTQLNQTGGPFGTP
                     IGGTPATITVIGTNFIDPMTVEVYGGGVTIPINNAIVANADQLSFTAPAVPDSALNEQ
                     PCIPAPNAGVTGVKYVPTSFGIRLRNARTGCVVELPNILIYNPPEPVVCRTPLTITTL
                     TLPAATICTAYAGATATATGGTPGYTWNATGLPPGMSIDLGTGAISGTPQLPTAGAGG
                     TTTLSVGLSATDGTATTVSRTIGLQLIDPNGQFSITAPASTTVSAAAGTQIGAFTLTP
                     NATPADFQPVNWVVTGPGANQVVINGSGVLSTSGTAVAGGPYNITVTATDTPSCGGAS
                     HTASATYTFSLN"
     gene            complement(27508..28089)
                     /locus_tag="PLP50_06865"
     CDS             complement(27508..28089)
                     /locus_tag="PLP50_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51307.1"
                     /translation="MKNRIPLAVALLAGVLAVAGCSSNTQGDDASPVYLSAEYTLLPE
                     SWNVGSGAPLQLETVQLRSHLKNTTGGTSSFLDTRLDDYVVEWRRIDGGTKAPEAETF
                     PGNVIVPAGGTSTMSNYPYMTRSALLKAPLDQLLPFNGGIDRETGNSEIRCRATVTYR
                     GRTLSGQPVRGVGTFGLTFLYSAATETIVARVN"
     gene            complement(28112..30787)
                     /gene="pilQ"
                     /locus_tag="PLP50_06870"
     CDS             complement(28112..30787)
                     /gene="pilQ"
                     /locus_tag="PLP50_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HPA51308.1"
                     /translation="MFRTGRFSAPGLAGILVAALAAAGCSSQAGPAPTTENVVSASVA
                     PSVLTSAALLIDGDAPRLLLAADGPLRPMVYAREGSKTLVVDLPGTVATPGLEPPRAD
                     GTLLTALEMRSFSELGQPQVRFELTGRGALEAQIAGDPATRAMSIALLPRIEEPILAQ
                     AETPAAPTAPAVVAQAAPPTVPAPAAASPSEPVVVARHEASGAVATRLARVATETRRG
                     ALAVRLEADGELSYEAFTLSSPPRFVVDLSGVRNASRFRNEDVDAAGVARVRVSQFRT
                     EPTAVTRVVFDLEKESLPVLRQSGSTVSISFGGEAEAPVQVAAAAPAPAPAVPAAGSY
                     EAHEADDLAEPIVPTAPAPEVVAAAPVVAEPVVHVPAAAELPAVAEAAPVVEPAAVEP
                     PAPAEPVVVAELPAPEPVAVKTPEPVRVAAAAPTVTVASPEVAVPAPVPPRPARRKTS
                     ADDRALLEAAETLAAEQQSGGQLKDISNPYESRAIGQTERQYTGEPLTLNLKDADIKD
                     TLQKFSELTNLNIVLDPDVRGTVTVSLTDIPWDQALELILKINGLGYVLEGNVMRIAA
                     TGKLAHEESARQNLLKAQEANRPTRTVIQKLSYASANSAATTAKKVMSPRGDIFVDER
                     SNLLIIKELPEYLPTVLDLVKSLDTPVPQVMIEARIVEATRTFSKRLGIDWGFQAISD
                     QAHGNTTGMVFPNSAEVEGAVSLPNGNQILRASLANVLDTFRLDVALSAAEARGLVKL
                     VSSPKILTQTNMAASVQSGFQIPVQTTVNNTTTVLYIDATLRLDVTPQVTAEGTVILD
                     IKIQRREPAPGVAITGGNNVPLVTRDAQTRLMVRDGGTGMIAGIFKLSANDGQNMVPG
                     LWKIPLLGGLFRNKTMDETTDELMIFITPRVMKAL"
     gene            complement(30874..31374)
                     /locus_tag="PLP50_06875"
     CDS             complement(30874..31374)
                     /locus_tag="PLP50_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51309.1"
                     /translation="MKLNRASAWLLAVSILPASSALGQAPPPAAPATPPTETGIEVPV
                     PGEQDTTAPAYTYEVRGRRDPFRSLLLRTQDDKDRQRPPGIAGMLVEELELQGTIRTR
                     QGWIAMMRGGDNRSYLLRKGTTVFDGEVMEIGPTEIVFRQNVNDPTSPKPFRDVVKAL
                     ALQAKP"
     gene            complement(31374..32045)
                     /gene="pilO"
                     /locus_tag="PLP50_06880"
     CDS             complement(31374..32045)
                     /gene="pilO"
                     /locus_tag="PLP50_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPA51310.1"
                     /translation="MAIELKQLEDKPWYYGLGIGLALAVGLWAVANWQYPNFKEMQQR
                     LAGLKAEYGKLQEKIERGRVAERRLPQLREEVRRIELDLNRLLEILPTKRNTEELIKK
                     IEALTRQGDFFLKDFRPKEYVNKDFYAEWPIEVVLDGTYHNLALFFDKMARFSRIINV
                     ESLTMTGYSDARLGRTLGANFTLKTFIYLGDQASGSGRATPPRGGSAGGGAAIPSGAG
                     RDGGV"
     gene            complement(32056..32709)
                     /locus_tag="PLP50_06885"
     CDS             complement(32056..32709)
                     /locus_tag="PLP50_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPA51311.1"
                     /translation="MIKINLLSEAKPQRKKKGVSALGGAGRLNLFLILGGLLIGLLVV
                     GVQWWVEASRLKEQQEKNRIAQQEVTRLEAVLKEVADFEDKKAKLQKKVDLINALKAN
                     QRGPVRLMDEVSNALPDLLWIERMQVKGNSITIDGKALNPPAVANYLTNLKRVSAFQE
                     PKVRAITAASAGASNLYNFGLTFVFADLDRTQFVTAEPDGSEAASAPAAPAAPAAGN"
     gene            complement(32723..>33468)
                     /gene="pilM"
                     /locus_tag="PLP50_06890"
     CDS             complement(32723..>33468)
                     /gene="pilM"
                     /locus_tag="PLP50_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPA51312.1"
                     /translation="RWEAEQYVPFDINDVYLDYVVLDPGVPGETMGVLLVAAKKEKVD
                     DYKNVVTQAGRTPGLVDVDVFALQNCYEVNYGLDPTRVIALVNIGASVMNVNILASGQ
                     TAFWRDITFGGNQFTDALQKAFSLNFEQAEALKKGEPVGGQSAESIQPVLAGVCEDVA
                     GELQKTIDFFHATSGSEKVDTIVLSGGGSRVGQLDEVLKDRFGLPVEIMNPFRSVVAE
                     DRSVDPAWLSENGPSLAIGVGLAVRKLGE"
BASE COUNT         4802 a        11777 c        12340 g         4549 t
ORIGIN      
        1 ccgcctcgac gagggccgtc gcgtcgaccg cgagggccgc gtcgacggcc gcctccagcc
       61 ccgcgcgctc cttgaccttc gtgatcccga cggaggagcc ggcgcaggcg ggcttgacga
      121 agagggggag gccgagggcc gccgcgcggg cgaggagcgc cgcgcggcga ctctcgtcgc
      181 gccaggcggc cgcgcggacg ccgacgtagc gcacctgcgg caggccggcc gctccgaacg
      241 tcgccttcat caggagcttg tccattccga cggccgaagc ggcgacgccg aagccgacgt
      301 acgggacgtt catcgcctcc agcagcccct ggaggacgcc gtcctcgccg aacgtgccgt
      361 ggaggatcgg gaagacgacg tcccagcctc cgtcgcggaa ggccgcctcg aacgcggcga
      421 cgatctcgga cggaggtgcg ggtctcgagc cggggacgac gccgtcgagg agcgcgtccc
      481 gcgccggacg cgcgaagtac cgaagcatct cctcgcgcga atcggcgacg ccgtgccatc
      541 ggccgtcctt gccgacgaac accggcaccg cctcgtatcg gtcggacggg aggttctcga
      601 gcaggcagcg cgccgagagg aaggagacgc cgtgctcccg cgacggcccc ccgaagacga
      661 ctccgacccg ggtgcggatg ctcatgcgcg ctccatgttc gtcgcctccg ccgtggagga
      721 ggctcgaagt ccttcccgcc agcgtaccgc aaacgagaac gtgccccggg gccgccgcgg
      781 ccccggggca cgtgggagct tctcgatcgg ccgggctctc gttcccgccg ctcagccctt
      841 cgacgcggag aggagcttcg cggcctgctc ctcgtaggcg gcgaggacgt tcttcaccga
      901 cgcgacgtcg acgtcgccca tgtgtccgat ccggatgttg agcgacttga acttgccgta
      961 gcccgagccc ggcacccagc ccgcgccctt catcgccttg ttcaggtcct ccgcgccgac
     1021 gccctcgggc gggaagaggg aggagacgct cggagagtgg gccggcgggt cgggaaggaa
     1081 gcggaagccg gccttcggcg cccactcttc gaccgccttc agcatctcgt ggtgccgcgc
     1141 ccagcgggcc tccatcccct cggcgaggat gtggtcgagc tggacgtcga gggcgtagag
     1201 gagcgggagc gacggcgtcg tcgggttctg cttcttcttg ccgaacgcct cgacgtcgag
     1261 gaggtcgagg tagaggccgc ggcacttctt cgcgcgggcc gccgcgagcg ccttctccga
     1321 gaccgcgaag accgcgatgc cgggagggag cgcgagcgcc ttctgcgagc cgcagacggc
     1381 gaggtcgagg ccccactcgt cggtctcgac ccgcgtcgcc gcgagcgacg tgacgacgtc
     1441 ggcgaagagg agggcgtcgc tgttctcgtg gacgaccttc gcgattgccg cgaggtcgga
     1501 gatcgtgccg acggaggtct cgttgtggac gaccgtgacg acctggtact tcttcgccgc
     1561 gagggccttc ttcacgtcgt cggcgaggat cggcttgccc cagtcggacc gcacgaagtc
     1621 cgtctcgagg ccgagccgct ggctcatctc gccccacttg tcggagaagg agccgttgca
     1681 gcacgcgagg acggggcccg tcgcgcagga aacgagtccg gcttcccaga cgcccgtcgc
     1741 ggaggtcgcg agcgtgtggg cctgcccctt cgtccggaag accttcggga ggttctcgag
     1801 gatgcggccg tagaggtcgg agaaggccgc ggaacggtgg gacatcatcg ggccgccgag
     1861 ggcctggagc acctgcggac ggacccacac ggggccggga ttgaagaaac ggacctgccc
     1921 ggcgtggccg gaggtggagt cgaaggcgga gctgctcatg ttcaggattt taccctcggg
     1981 gtgcgcgctc gaagcatcgc gaaacccttt gtgagccaac ggcggagccg ggccgaccag
     2041 tccatttcgg ctcacttcgc cccctccatt gggatcagcg tcccgtcgga cgtgacggcg
     2101 tcgttcacga caccgtaggc gacgaagcgt ccctcgccgc cgacccggcg cacctcggcc
     2161 caggcgtcgc cgccgtaccc gacctggccg agcggacggt ccggctgagc gcggcggccc
     2221 ggagcgagag tcatccgcgg aagcgtcccg atcaccgccc cgtccgccgc ggcatggagc
     2281 gtcacttcga gcgtcacctc cggcccgccg tccgcctcgg ggttcgcgag tgcgaggttc
     2341 gagcggaagg cgccgtcctc ccgcaggccg gggacgatcg cccgctccct cgcccaccgc
     2401 ccttcgctca cgagcgggac gctcacgccg tagtccccct tcgcgcccgg ggcgcgcgcc
     2461 tggacgacgg ccgtgacgag gagcgcggcg gccccctccg gacgttccgc cgtgaaggag
     2521 agcgtgccga tgaaggcctg cgggtcgatc ggcacgccgt tcgcgacgag ccacgcgccg
     2581 gcgtcgggga cctcgaggac ctcgccgaac agcatcgcgt agggagtcga gcccgcttcg
     2641 aggaccaccg ggaacgtctt cgacacggcg tcgtcgcggt aggaggcctg gaactcgagg
     2701 cgggggacgc gccggccgtc cgtcgatcgc gagccgagcg tcagctccgt cctgtaggcg
     2761 gcgtgcgccg aggtgacgcc gacgacggcc ggcaggaagc gagtccgacg gcccgggatt
     2821 gcatccggcg gcgcgaacgg aacgacggtc ccgtcgttcg tcgccccgtc gttccggacg
     2881 aggtagccga ggaggtccat cggctcgtcg gtcccgcctg tcgaacccgc gatggggctc
     2941 ggagcgagga cgagcggccg ctcgacagcc tcgggatggg gatcgacctg gaggaatcct
     3001 cccctcggca ccttcgcgag agcagtctcc tcccacggca cggccgacct ggggtcccag
     3061 acccacaggc ccgctccggt gccgtcgttc gtcgccgcca tcgcgacgtg aagccggctg
     3121 ccgggagtcc tcacgggccg cagcgccgtt ccgtcggcgc cgacgccgcc gggatcggtc
     3181 gcgacgagag cggttcccgc cgtgcccgac tccgaggggc tccagactct caccgccgcc
     3241 caggcctccg actcgtcctc gagaccctcg aactccacgg tcaccgggcc gatgaagccc
     3301 gggaaaggat cctcgatgcg gacctgcgct cccggacgga gcgccagctc gcgcgcgggg
     3361 acgacggccg tccccggaaa gagccgggcg acggcggagc gggacggcga gaggttcgcg
     3421 acggagaggc tgctccggta gcgggtcccg ccgacccccg tcgagtcgac cacggccgcg
     3481 aggtgcttgc gcactctcgg caccgggacg aggagagggt gctctccctc gtagcgccgc
     3541 gcgccatccc agccgaagcc tcccgagggg gcgatcgcac gcggcgcaag cgggcccatc
     3601 aggtcccgca ggcgaccgcc ggagaaggcg agctgcgcga agaacgccgt gtcgccgacg
     3661 gcggcgatcg gcgccgtcgc gccggggctc aggttcgcgc ttcgcaggtc gttcagggtg
     3721 agcagcgtcc ggaacaccgc gccgtcgaac gagacgagcg ccgccgcggt cccgtcctcg
     3781 tacgctcccc gtacggcgag gaaggtgcgt ccgcccgcgg aggccgcatc gagaacgcga
     3841 agccccgggt cgaggcccgg gaatgcctcc tccagccgct cgttcctcag gacccaggcc
     3901 gagggccggg tctcgcccca ccacccgaag aggtgcggca cgccgtcgag gacgcgaacg
     3961 acgctgcccg aagagcccgg ggtgggagcc tgcggcggcg tgaagatcgt ccagacgccg
     4021 tcacggcagc gtgccaggcg gttcccggtc ccgttcaccc agaccgcgtc cgccgttgcc
     4081 gccagcgagc gctggagtcc gttccaaagg ctcgcgtcga agaggccgaa gccctcgggc
     4141 agcttcaggt cacgccaggt gtcggtggcc gggtcgtgct cgagaaggtg gtccgtcccg
     4201 tcgtcgacgg cgacgacagc cctctcgccg accgcgacgg cgcccgtgat cgaccctttg
     4261 gccgggagcg gcgcgagtcg ggaccatccc gtttcccctc gccggaagac gcggttcccg
     4321 acggtcgcga atcgccgcgt gggactcgcg aagtacgtgt ccgccccgat cgtctcgccc
     4381 gctcccgccg tcggcaccca ggtctccgcc tcgcggacgt ggacgtccca gccctgcgcc
     4441 caggtgaaga ggcaccgtcc cgcgacggcg acgctcgggg cgagggacct ctctccttgg
     4501 accggagact ccggcgtgag ccgctcgagg cgcccctcct tcagctcgta gatgttcccg
     4561 cgcgcctccc ggctcgacgc gaggagccgg ccgttccact cgaacgggcg gacgccgtac
     4621 gagacgtcgt agacgccctg cgggtcgggg atcggcgcgg agaggccggc cgcggtgagg
     4681 acgcgcgcct cgatgcccgt cgggccgacg gagtagaagc cccagaggct gttcggcgag
     4741 gagcggaagc cccagccatt ggccggccac ggctcgagcg tcgtccagtg atcgccgtcc
     4801 agccgatgga acgggatcgg agcctccgcc gccgcggcga ggctcccggc cttcgagctc
     4861 ggagcggcgc agccgtcgac agctcctccc cactccagga gatggagctt cccgaagagg
     4921 acgaagagcc ctccgttccg ggccgggagc gacggttcct cgcggatcga cccgtcggcg
     4981 aacgagatcg agcggagcct ccggccttcg acgtcgtgga ccccggccgg gtccggctcg
     5041 cagtcgttga accggagcgt ggagacgtag agccggtcgt ccgacgggac gtagtcctgc
     5101 ccggcgtcgg gcaccacccc gagacggacc caggcgttct cgcggaggac gaagacctcg
     5161 atgccgccgc tccggagctt cagggcgacg aaccggccgc ccgcgacgaa ggacggagcg
     5221 aggctcccgt ccggcgaggc cgaggcgtcg agcgagatcc gccgccagac cgtcccgtcg
     5281 agctcccacg tcccgccgga gccggacgcg aggacgcgtg cgtcgtcgcc gacgatcccg
     5341 accgagccgg gcgcacgcgg cccctcgtcg gcgaccttcc agacgaggtc ggcggccgac
     5401 gaaagggcga gaagcgaacc aaggaccagg atcgcggcac ggcaaacccg gagcggcgtt
     5461 cgagccatcg tcatccccct gttgacggga gtgtctcgga acgccgacgc ctcacgcgga
     5521 accgtacact tcctcgaccg cgccgaccgg ccggtgacag gccctcgagc tcgtcgcccg
     5581 gcgagggacc tcagtgagcg cgaacctcct cgaacaaccc gaggacctcc gaaagcgcct
     5641 cgaggagatg gtcgggccgc tgggcgagga gctcctcgcg ggtcgaggag ccgagcggga
     5701 ccacggcgac ggagagaccc gccgcacggg ccgtcgcgac gtcgacgacc atgtcgccga
     5761 cgaagagcgt cccggcgggg ggcacgccca tcgcgtcgag cgcggagagg accatcgacg
     5821 ggtcgggctt ctcgggaaag ccgaggtccg gcccgccgac gaacgcgaag agctcctcga
     5881 ggccgaagcc ggcgaggagc tgccggctga agacggccgg cttgttcgag gcgatggaga
     5941 gcttcacccc ccgcctccag agctccgaga cgacgagctc ggcgtcgggc atgaggcgcg
     6001 tcagcttcgg cgcgttcacc ttgtagcgga agcggaagcg ttccgccccc tcggcgcgga
     6061 gctcctccgg gaggaccttc gcgatgagcg cctcgaggcc gtgcccgacg agccgcctcg
     6121 cctcctcgac ggtgaccggc gggcggtcga agtgctcgag gacggtcgag agggcgtcgt
     6181 ggattccgac gtaggagtcg aggagggtcc cgtcgaggtc gaagacggcg gcccggtacg
     6241 tcacgagacc accgtgagga cgatcttccc gacgttcgcg ttcgacgcca tccggcgatg
     6301 ggcgtcggcg gcccgctcga gcgggaagac ggagtcgacg aggggccgga agcggccgtc
     6361 ggcgaaaccg ggaaggacgt tccggacgaa ggccgccgtc aggtcggcct tctcggcgag
     6421 agggcgcgcg cggagcgtcg acccgacgat ccggagccgc ttcgtcagga cggcgcgcag
     6481 gtcgagctcg gccgccccgc ctcccatcgt cgcgatgagg acgagccgtc cgccccgcgc
     6541 gaggagccgg acgttccccg cgagcgcgga gccgccgacc gggtcgagga cgagatccac
     6601 cgccgccttc ccgaagcact cctcgacggc ctcgacgaag tcgcgcgccg tcgtgtcgac
     6661 gacgaggtcc gcgccgagcg cctcgagcgg gcccgccttc gcggccgagc ggacggtcgc
     6721 gacgacgcgc gccccggcgt ccttcgcgag cgggatcgcg gccgtgccga ctccggaagc
     6781 ggccgcgtgg agaaggacga cctcgcccgc ggcgaggcca ccctctcgga agaggttcag
     6841 gtacgccgtg aaccatgcct ccgggaccgc cgcggcctcc tcgaacgaga gccggtcggg
     6901 gatcggcatc agcatcgagc gcgggaccgc gaccttctcc gcgtagccgc cgcccggcag
     6961 gagacagaag actcgctcgc ccgtcccctc gacgaggccc gccgcctcga ggccgaggat
     7021 ctccgactcg cccggcggcg gcgggtactt gccggccgcc tggaggaggt cggcgcgatt
     7081 gacggccgtc gcgcggacac ggacgaggac ctcgccgtcg cgaagggccg gctccggcgc
     7141 ctcggccggg acgagcgcct ccgggccgcc gggagacggg acgaggatcg ccttcatcgg
     7201 ggacctccga ggccgagcgc gtcgaggacg agcgggacga gccgatcgac ctcgccgagc
     7261 ggcaggcgcg gcacggagga ggcgtgcgtc gtctcgcggt ccgtcatcac ggcgaggacc
     7321 tccgggtcgt cggcggcgac cggctcgcgg ccggtggccg cccggcagac ctccaccttc
     7381 gggccgggcg cctcgcggaa gccctccacg acgacgacgt ccacgtcggg tatctcgcgc
     7441 gcgacgacgg cgtcgagcga cgggtcggca tcgtgtcggg cgtggacacc ggtcctccgt
     7501 ccggagacga agacgacggg gctcgccccc gcggcgaagt gccgctgcga gtcgctccct
     7561 tcgacgtcgg tctcgtgctc gtgacgggtg tgcttcacgg cgccgacgga aaagccccgc
     7621 gccacgagcg cctcgacgag cccgagcgcg tgggtcgtct tccccgagtc ctgcgcgccg
     7681 acgacctgga ctcgcggcgg cccggcgcgg tagaggagcg ccgcctcgcg ggacggggcg
     7741 aagcgcgcga cgtcgcccgg gaacgtcgcg atccaagcgt cgaggctctc cccggcgtcg
     7801 acgagctcgc ggtagcgcgg cgtccccgcg aggaggtcga cggcggggac gtcgctccga
     7861 tactcgtacg tctccgtccg ccagcggaag cgggacgggt cgaggtcgcg caggagcttc
     7921 acgagccgga ggcccgtctc gtacggccgg aacgcctccg tgtccgtgac gtggagctgg
     7981 acgccgccgc agtcctgccc cgcgaacttg tggaacgtcg gccggaagac gtggggacgg
     8041 aagaggacgc cgggaaggcc gagggcgttc gcacggtcgg cggcggcgtc cgggtcgagc
     8101 cacggcgccc cgaccgtctc gaacgggcgc gtcgtcccac gcgcctcgga caggttcgcc
     8161 ccctcgagga ggcacatccc gggatagacg agcgcggtgt cgcgcgtcgg catgttcggc
     8221 gacggaagga cccagtccgg cgtttcgccg acgcgacccc gcggcgcggg ccgcccgtcc
     8281 acgcgcatcg gcacgacggt aaggtcgcag gcgagcgcgc tctcgacgtt cgcccagcgg
     8341 gcgagctccc ccggcgtcat cccgtggcgg gccggcacgg ggcggaggcc gacgaacgag
     8401 aggagccgat cctccggaag gttcccctcg acggcgaagc cgccgagcgg gttcggtcgg
     8461 tcgaggacga agacgggctt ccccgccgcg gcgcacgcct tcatgacgag gaacgtcgtc
     8521 cagacgaacg tgtagtagcg ggagccgacg tcggggaggt cgcacacgac ggcgtcgagc
     8581 cccgcgaggt gctccggctt cggagagagg tcctcgaacc gatctccgta cagcgagacg
     8641 acggggaggc gcccgctcgg cgtgtcgtcg gccccgacgg cgatcatgtc ctggacggcg
     8701 cccgtcagcc cgtgctccgg gccgaagagg agcctcaggt cgagaccggc ctcgacgaga
     8761 gcgcgcgccg tcgggacgaa gcgtgacgtg acggaggccg ggttcgcaac gaggccgagc
     8821 ttcaggcccc ggagcggggt cgggtccgcg aggaggacgt cgaggccgga gaggaggcgg
     8881 ggtctcgtca ttcggctgct ccccttgcgc cgagcgtcgc gacggcggcg ccccgatagt
     8941 aatgcgcgag gatcgcgtcg cggctctcgc cgcgccgggc ccgtgcgatc gcgccggcct
     9001 ggcagagccc cgcgccatgc cccgtcccgc ggccggtgaa ggcgtagccc gcggggcgct
     9061 cctcgacctc gaagtctgtg gagcggatcg agctccagcc ccagagctcc gaggcgcgtg
     9121 ccctcacgtc gaaggcgcgg acgacccgcg accgcccccc gggagcggcg aggcgcaggg
     9181 cggcgacgcg gccgtcgtcg gcatgggccc agacctccag gaagcgggcc gcggggaagc
     9241 cgagcttctc cgcgaggagc ggcaccttcg cgcgcgggag gaagaaggtc cagcgtgccc
     9301 ccggcgacgc gaggcaggca tcgtcctcga ccggcgtgag ttcgggcgtc tcctcgtcgt
     9361 cccagatggc ggaggggcgg gcggtccggc cgccgcagac ggcgtggaac ggtgcggcga
     9421 cgacgtgacc tccctggacg aggacgaggc cgcgcgtcgc cgcggcggcg cgccgcgtcg
     9481 cctccgtcgc ccgcccgagg ccgcggaaga cctggcagtg gaccgagtcg cagacgtccg
     9541 cgccctggtc ggcgtgacgc cccttctggg cgaccgcgta cgagcgcgcg gcgacggcct
     9601 gcgcctcgag cgccgccgga ggcgcgaagc tcccgatctc gtccgggagg acggccgcga
     9661 cgtacgcctc gagcgggacc gtctcgacgg caccggtggc gaggcggagg acgcgccgcg
     9721 tcgtctcctc cgccacgtcg acgggaatcc gggaggcggc ccgggccgga cggagggccg
     9781 cggcagcggc gaggaggagg agcgcgctga acgcggcggg gcgagcgagc ttcacgcggc
     9841 ggaatccccg gcgaccgctc aggcgcggag ggaggtcggg tgcgactccg cgccctcgcc
     9901 gcggagcatc gcgaccgcgt gcggcaacgc cgcggcgatc gcctcgaggc actccaccgc
     9961 cccgcgcggc gagcccggga ggttcacgac gagcgtcttc ccgcggatcc cggcgacctg
    10021 ccgcgagagc atcgccgatg ggagcgccga acggctcgcg gcgcgcatcg cctccggcag
    10081 gcccggcagc tcgagcgaga tgacggagcg cgtcgcctcc ggcgtcacgt cgcgctcgga
    10141 gatccccgtc cctcccgtcg tcaggacgag gtcggcgagg acgttgtccg cgaagcgggt
    10201 caggtgccag acgatgtcgt ccttctcgtc cgggacgacc tgcgtctcga cgacctccgc
    10261 gccgaggagc cgccggacgg cctcggcgac cgccgggccg ccgcggtctt cccgctcgcc
    10321 gcgggccgag cggtcggaca cggtcaggac ggcgacgcgc agcgggacgg acctcatgcc
    10381 gacctcggtg aaaagcagtc cggcgcggcc ccgcagaagg cgactctctc tgacatggac
    10441 gggattctat gtggcctcta ccctccgctt gcccgggact cccatcagac ggtcgggccc
    10501 ggacagcgcc gtgaatcgcg ccgaccggca cagggcgccg ccgaacgctc acggagcgcg
    10561 tcgcgccgag ggcgccgcac ccgctccgct cccgccggtc ccgcccaggt cgaagagctc
    10621 cagactctcg cccgcgcgag caacgaggcg cgagccgggg gctcgggcga gggcgagaga
    10681 ccgggccgcg cgcccggacg tgtcggtgac gagcacggcc tcgggaccga gcaggaggag
    10741 gtggcgggac accgcgaggt accggcgggg cggggcgctg ctctcgagca ggtccgcgac
    10801 ccccgggatc cgcagcgagg ggtcgtcgcc gccgaagtac gcgatcccga cctgcgacga
    10861 cgggatcccg cgccgggcga ggagatcgcg aagacgaagg aggtcctgcc cccagtcgag
    10921 gttcgagtcg gcgaggacgg gacgaccgac gagacgcccg acgagaccat cggtcccgag
    10981 gttcctttcg gacgcgagcg gggaaagcgc gacgaggagc agggcggcac gcgagacgcg
    11041 cggaagcctc gagagagaag ggcccagcgc ggcccccgcg ccgagcgcca ggaagacgat
    11101 cgacgggaag acgtggcgca cgccgatgcg cggcccgggt agcgacccgg cgaagagcag
    11161 cagcgcggcc acgtaggaga gccgcgcgaa ccggggcccg ccgcgagccg ccaggtacgc
    11221 accggcaagg acaccggcga gccagacgac cggggacttc acgacgagcg cgacgacatg
    11281 gtagtgagcg cccggatgcg acgtcgggcc gtcgaagtag gtgaggcgtc cagcctctgc
    11341 ctgcctcaga tggaagagga ggccgaaccc ccagcgagcc agtccgcgat ggacggagtc
    11401 cgcgcgctcg agcagcgcca ggtcctcctt cggcatgcgg tacaccctcc cgaggacgcg
    11461 gatcatctcc ggctcctcgc cgcgcacgga ccacgagagc gccgccgcga ggatcaggat
    11521 cgccgccacg ggaacaccgg ccgccgcgag gaggtccctt cgggtcggtc gtccgtcgcg
    11581 ccgagccaga agggcgagga cggtgagggg aaggaggagg atcgccgagt acttcgtcgc
    11641 gagcgccgcc gcgagccaga cggacgccgc gagggcggga cgaagccgtc gggcctcgtc
    11701 gaaccgccgg agagagccgg cgagcaggac cgtgaacagc gcgagcggca cgtccgtcgt
    11761 gacgaggtgt ccgtgcggga agaccgcggt gttcccgacg agaagcgcgg aggccgcgat
    11821 cccgccgggt ccctttccgg cggcggccca ggccccgacg acgagtgccg cgaagaggag
    11881 cgccacggac acccgcgagg cgaacaggac ggagtcgtcg cggctcgcga agagccagct
    11941 cctcgtcgaa cgaaagaagt cgcggaagtc caggcgtggc ggctcggccc cccccagccc
    12001 gacggccgag atcccgacga gctgcttcgc gagcggagga tgctccacgt tgacgagcca
    12061 cgtcccgctc gtgacctgcg agacgcccgc cgccgcgtgg atcggctcgt cggccgtcgc
    12121 agaatcccgg cgagcgtcca ggagtgagag gcccagcgcg agaagagcca gaagcgcgac
    12181 gacgagtcca tcggggaaag ggcgcctcca cgcggtccgc agcgcagtcg aggcccccgc
    12241 aggccgaaga ggaccttccc cgagcccgtg agcgtccgtg gagcgcccgc ccggcgcggg
    12301 ctccgcgaag acggcggtcg tcgccggggc acggacgcgt cggacgctgc cccccacgga
    12361 aagtgccgcc ccgtcgggga cgatcctcgc ggccatccga ggaattctag gcgcacccta
    12421 gaatcggggt ggagcccgac accgtgcatg cccttcgcgc ccgccgcgcc gtagcggccc
    12481 tcctcgtcgg gacgggcctt ctcacgctgt cggccggcgc tgtccgacac gacctgtcgt
    12541 ggccggactc gttgcgcatg gcccggcgct tctggcgagc cggacctcgc gggcgtctgc
    12601 tcaacgcacc cggaatcgcg cgagatgccg gtttcgcggc cgaggtcatc cgcgcggatg
    12661 cgatctggcc acgcgacgaa gacgtcgtcc tcaccgtcgg ccgggagctc gcgcccgaac
    12721 ggagggagcg ggtgcggcgc accaccgcct accttctcgc tccccgtcgt gtcctcctcg
    12781 aggtcggcca gggtacggag ctgcacctcc ggcccgtgag cgcgaagggg ccggcgcgat
    12841 gagacggctc cccgccgccg ccgccgggct cctgctcctt ctcggtctca cgtccgcgac
    12901 tttcatcgcc gtccggcaac ccctttcgct cggcgactac ctcgcgatct gggggctcaa
    12961 ggcgcgcgcg atccacttct ccggcgagct cgattccgtc ttccgcgtcg acccggaggg
    13021 ggattactcc catcccgcat acccacccct ctggccgctc ctgctatcgg gtgcctcgcg
    13081 cctgatcggc cgatacgacg agcttctcct ctctctcgtc cggccggtcc ttctcgtctt
    13141 tggggccgcc ctcaccttcg cccgaacgcg cggcacgacg acctggcggc tcgttgccgc
    13201 cgcagcgttc acgctccttc cctacttcca gagcgccgcc tacaccgggt acgccgagac
    13261 gctgctcgtc gttctcgtcc tcggcgcgct cctcttcctc gagcggtccg ctccgggctg
    13321 ccgggcgtcg accgcagccg gggccctgct cgcgctcgcc gctctcacga agcaggaggg
    13381 gatcgtcgcc gtcctcgtcg tcgcagcggg actacttctc gcccggaggc gtggcgacgc
    13441 cctgcgcgtc gccacgctcg gcctcggtct cggcgtcctg ccctggagcc tctaccgagc
    13501 cgcgcactcg gtccgcgcct tgtccgtcca cggggcgtcg gcgttcgacc tggagaagcc
    13561 cctccgcgct ctctccaccc tcgcgacgga ggtcgggtgg ccgggcctgt tctggtccgc
    13621 cggggcggtc gcgctcctgg tcgtcggtcc cgtcgcgcgg cgcgaccgcg agcttcttct
    13681 cgcgggcacc ctcgcctacg ccgcgctcct cacggcctcg ttcgtgttcg cgcggatcga
    13741 cgtctcctgg tacgtcgcct ggtcctggga ccggctcgcg ctcctcccgg tcgccgcgct
    13801 cctccccgcc ctcgccgagg ccgcggcgga gccgttccgg acgggagaag acgtcgcggc
    13861 cgaaggctca ggcgcggcgg cgcgcccctg aaggggccga ggctcgtcgc cagtctccgc
    13921 tctttccgcc gctcttggcg acgagctcga tcggcccgac gacgatcccc ttctccgccg
    13981 ccttgcacat gtcgtagacc gtcagggccg ccgccgccgc cgcgaccatc gcctccatct
    14041 cgtagccggt ccgtcccgag ccgcggacgg tcgcgaagac caccacctgc cggcggctcc
    14101 gacgtcgctc gaggcggacc tcgacggcgt cgaagaggag cgggtggcag agcgggatcc
    14161 agtccgacgc tctcttcgcg gcctggatcc cggcgattcg cgcgacggcg agggcgtctc
    14221 ccttccggtt ctccgaggcg tcgagggcgg accacgccgc ggccccgagc gtgatgaccg
    14281 cgcgggccgt cgcggaccgg accgtctccg acttgcccga gacgtcgacc atccgggccg
    14341 caccctcgga atcgagatgc gtcaggtcag gcttcttctg cgagtgcacg gaggtcctcc
    14401 gggctgttca cgttgagaaa gactccggga cggaagccgg gcaggcgctc ggtctcctcc
    14461 gccgagagga cgagggcgcc agccgactcg agggcgcgcc ggagggagag gtcgccgccg
    14521 gcgaccgagg cgcgcagcgc cgggagcgcg cgtgtcgacc aggcggagca gagcgcctgc
    14581 gggccccgcc cgtcccacgg ggcgacggcc gcggcgccga ccgcatcccg ccgcgcccgg
    14641 agcgcctcga ggagctcggc gggaaccagc gggagatccg ccgcgaggac gaggcattcc
    14701 tccaccggag aagcggcgag ggcggcgacg acgccgtgga ccgcggcccg ctcccgggtc
    14761 ccgtccgcga cgaaggggtg gccgagcgcg gccagcgacc ccggctcctt cccgacgaag
    14821 gagaccctcg tacagatcgt cccgagcttt ctcgcctgaa ggagggccat cgggacgccg
    14881 ccgatcggca ggagcgcctt gtctcgcccc atccggctcg agagcccccc gacgaggacg
    14941 aagccgtgcg ccacgagccg gcatcgtaac ccgccggcgc acggcgggga gccgcctaca
    15001 ccagcttcag gaggtgcccc atcttcgcct tcttgacgga gaggtagccg cgcgtcgcgt
    15061 cggtgggcgg gatctcgagg gagacccgct cgacgatctc gaggccgaat ccgtcgaggg
    15121 cgacgtactt ggacgggttg ttcgtcagga ggcgcatctt cttgacgccg aggtcccgga
    15181 ggatctggca gccgatgccg tagtcgcgca cgtcgggggc gaagccgagg gcctcgttcg
    15241 cggcgacggt gtcgaggccg ccgtcctgga ggtcgtaggc gcggagcttg ttgaggaggc
    15301 cgatcccccg tccctcctgc aggaggtaga ggaggatccc ccgcccctcc cgggcgatca
    15361 tctcgagcgc cttgttcagc tgctcgccgc agtcgcagcg catcgagccg aggacgtcgc
    15421 cggtcaggca cgccgagtgg accctcacga ggatcggctc ctcctcggcg atgtcgccca
    15481 tcacgagggc caggtgctcc tcgttcgtca ggtccgaacg gtaggcgtgg acgcggaact
    15541 cgccgtgccg cgtcgggagg ctcggcgagg cgacccgctg gacgagtcgc tcgtggtgca
    15601 tccggtgccg gatgatgtcg gcgaccgaga cgagagggat cccgtgcttc tcagagaagc
    15661 gaaggaggtc ggggacgcgc gccatcgcgc cgtcgtcgtt caggatctcg cagatcaccg
    15721 ccgccggaga caggccggcg atccgggcga ggtcgatcga ggcctcggtg tgccccgcgc
    15781 gcttgaggac gccccccttc cgggcccgga gagggaacat gtgtcccgga cggaggaggt
    15841 cgtgcggctt cgtccgcggg tcgacgatcg tccgcacggt ctgggcgcga tccgccgcgg
    15901 agatgccggt cgtcgtcttg ccgcgcgcct ccaccgagac ggtgaacgcc gtcccgtggt
    15961 tcgaggtgtt ctgctcgacc atcggggcga gctcgagctc gtcgcagcgc tcctccgtga
    16021 gggagacgca gacgagcccg cgcccctccc gggccatgaa gttgattccc tccgccgtga
    16081 cgtgctcggc cgcgaggcag aggtctccct cgttctcgcg gtcctcgtcg tcggcgatga
    16141 tgacgaaacg gcccttccga tagtgctcgg cggccgccgg tacggtgacg aaacccaagg
    16201 aagaacctcc gggccgcggg gagcggctcc gcgtcaattg taccggggcg gcgccgcgcc
    16261 ctcggcgtcg tcgcccgtgc gcggccggcc cccccgaaac gggtgcgagg agaggaccga
    16321 ccgcttcgag acctcccgca cgagcgtctt gaggaagacc tcgaactggt gcgccgcggg
    16381 gaggaacgcg cgcgggctcc gctcacgcga gaagccggcg tggaaccggt cgaagaggga
    16441 ccagtccttg aacatgaggt gcttcatcga gccccgccgg aacgcgtcga gggcgtcgat
    16501 catcgctttg agcgccttcc tgtcggcgcc gcgggagaag gcctggaccg cctggaggag
    16561 ctcccagagg tcgtcgcgga ggcggaggct ctggtccagc ttcgtccggt actccgggaa
    16621 gagcgcccga ccgtcgacgt cggaggagaa cgtctccgcg aggccgatga tgctctgctg
    16681 gaagaggtcc ttgaggatcc ccgcggcgtc ctccatccgc gcgtagacga gcttcgggtc
    16741 ccgcatcagg gccacgtcga ccagctcgag ctccatcacc ttgcgcatct ccatctcgac
    16801 ggagaagacg aggcgctcga ggcccgcggc caccggcgtg tcctccggga agcgcggaag
    16861 gagccgcttg cggatgaact cgccgaggct cgccgcctgc gcccggatga ggctgaagac
    16921 gaggagcgcc cgcttgaccg tcgcgggcga ctcgatcgtc tgacggatcc tctccgtcca
    16981 gcgcagcccc ttgaagagct cgacgaagag ggccgccagc gccgacttct ggctcgcgtc
    17041 ggagacgccg gcgaggacgc gcgccagcga ctgcttcgac tcgcggtcga ggacgggccg
    17101 gaggttctcg cggaagagga cccccaggtt gggctcggac ttgagggtcc cgacgacgat
    17161 ccgcccgagg gagaggtagg tctgaagagg caggaacggc gcccgcgaga gctcgtcgag
    17221 gaccttcgag aagtcgtcga gcttctcgac gacgagggcg agctccgtct cggcgctccc
    17281 gcggcgctgc gggacggggg cgcctcccga ggcgcggctg acctggctgt cgaggaagag
    17341 gaggaacgac gcgaggtcct ccttcccctc cccgaggagg tgcgtcgtca ggagcgcgag
    17401 gtgcgagatc ccggacctca cgaccgcgac ctcctcgacg aagttcttca gcgacgcgtg
    17461 cgacctctcg aacgcggaga gcgggaggtg gtcgaggacg aagaaccgct cgaccccctt
    17521 cagccacatc tccatctcga agaggtagtc gtacttgacg cggttcgaga agagcgcgga
    17581 ggtctcgaga accgtgaagc gcggcatggc tgccgtcagt ttacgccgcg ggggaagcct
    17641 gcggaggggt cctcgccggg cccgggctcc tcgcggacgc ggaccgagag gagccggtcg
    17701 ttctgctcga cccggtcgac gacctccatc cctgccgtca cctcgccgaa gacggtatag
    17761 gccgcatcga ggtgcggctg gcgggagagg gcgacgaacc actgggagcc gccggtgtcg
    17821 gggccggaga gcgccatgcc gagccggccg cgaacgtacg ggatcgggtt cagctcgtcg
    17881 cggatcgcgt agccggggcc gccggtcccg tccccgcgcg ggtcacccgc ctggacgacg
    17941 aagtccggga cgacgcggtg gatcgtcgtc ccgtcgaaga accccgcgcg ggcgagagag
    18001 acgaagctct cgaccgtcat cggggccgcg tccgcgaaga gctcggcctc gaaggagccc
    18061 cggctcgtct cgacgacgac gcggacggga ggcccctcgg cccgttccgc gaggcgaagg
    18121 gtgtcggctg cggcgaggcg cgtcgcgacg gcgggcgacg ggtccgtcga atccaccccg
    18181 aagcgctccc ggaggaggcg ccgggcccgc tcgcgaacga ggtcgtcggc ggcgtcgcgt
    18241 ttcgcggcaa ggagctcgcg cccccccgcg ggcagggacg cggcggcgtc gagcgcggtg
    18301 gcggcgaggt ccgcctcgcc cgaagcgacg agcgcgtcga acgccgcgcg ccacgccgcc
    18361 gcgagcctcg cgtcgctccc cgtccccgcc gcgaacggag ccgtggcgtc gagcgcggcg
    18421 gcgcggacgg ccccgtccag gtcggcaagg gcgcgcgtca ggagcggaag gctgcggggg
    18481 acgagcggcc gtgggaggcg ggagaccgcc tccatgcgga cgcgcggcgc ggagtccccg
    18541 aggagcgcct cgagccaggg gccgacgcgc tcggcggcca ggagcgggag cgcttctgcg
    18601 gcgccgaggc ggaggtcgag aggggcggtc cccttcccgg gaaacgcgag ggcatgggcg
    18661 cgctcggcgt cgcccgcggc gaggctcgcg agggcgacgc ccccccggcg cccgccgtgg
    18721 acgacgaccc gttcgagcag cgcgcggacg tccgcgtcgt cggccgcggc tcgcaggagc
    18781 gtgagtgcgg ccagggagac gccggggtgc ggatcgcccc gccgtgcgag ccccgcctcc
    18841 cgcgcgacgt ctcgagcaag cccgcccgcc gcgagccgct ccagggcgag gagcgcctgg
    18901 atcgccggcc cgggctccgg cccgagcgcg acctccgaga gcggccccgc ggcctccgcg
    18961 tcgcccagga gaccgaggcc gcgcgccgcc caggccacga cctccgggtc gccggctcgc
    19021 agaagggcgc gcagcgccga ctcgctcccc ggccggggga cgcgcgcgag cgaccaggcc
    19081 gccatcctgc gcgtctcccc gtcgggcgag gccgccgccg cggcgagaag cggcacgagg
    19141 cccgggtcct tcgaacggta gagcgccgcc gccgccgccg cgaccggtcc ggaccgtgcg
    19201 cgcaggaccc cgcggagcgc ctcctcggcc gcgtcgcccc cgaggcgtcc gagcgccgaa
    19261 gccgcggccg cggcggtccc cggatcggcg tcggagagcg cgctccgaag gagcggaacg
    19321 agcctccgtt cgccggcgag gccggacgca aaggccgccg cccggcggac gcggggctcg
    19381 agatcggcga ggagcgtcga aagcagaggg accgcgccgg tcgcccggag gcgtccggcc
    19441 gcgagggcgg cccgctcgcg aaggcgcgcg tcaggcgagg cagcggcggc ttgcccgggg
    19501 gcgtcgtccc aggctcgccg gtcctccatc tcgaggagtc gcgccacgag cgcgagcctc
    19561 tccggccgcg cctcgtgccg atcggcatcc ggagcgacgg acgagcaggc cgtgaggacg
    19621 agcgccagaa tcggtagcga ggccgccccg acccgccggg cgccgccccg cagagccacc
    19681 ttgcgcctca gagccccagg aggagccggt cgccgaggat cgtcggaagc ccggcccgca
    19741 ggaccttctg ccgcgccgac ttcacgtcgt acggaacccg gtcgaacgtg accgtccgct
    19801 cctccgagtc gaagatcgcg tagctcgcgc gcgggttgcg gtctcgcggc tggccgaccg
    19861 accccgggtt cacgaggtat cggcggcccg gctcgaggaa gagcttccgg cggcttccgc
    19921 gtaccgcctc gacgaggatc ccgtccgggg cgagcgtgaa gatcgacggg atgtggctgt
    19981 gcccgaagaa cgagacggcg acggacggcg ccacgcggtc catgtggacg aagttcatct
    20041 gggcgtccca gtccgagaag atgtaggcgt cctcgtccag cggcgagccg tggcagatcg
    20101 cgaacgtctc gtccacgagg accgggccgc acggcagccg gcgcaggaac tcgaggttct
    20161 ccttcgagag cttctcggcc gtccagcggg ccgcgaagag cgcgggcgca ttgaacatct
    20221 cgccggagtc gacgcccgag acgaccttgt cgtggttgcc gcggatcgag acggccggac
    20281 ggggcagagt gcggatcttg tcgaggacct tgttcgggtc tgcggcgtag ccgacgaagt
    20341 cgccgagaca gacgacgcgg tcgagcttct tgcgcctcac ccgggccagg acggcggaga
    20401 gggcctcgtc gttcgagtgc aggtcggaca ggaggaggta gcgcacctcc tcagccgact
    20461 ccccggctcg cgagcgcctc gacgatcaga tcgaccacgg cgtccacccc ctcgtcccct
    20521 tccaccgcca ggatcgtgtc ggaaagtcta taggaagcgc gccgctcgtc atagagagcg
    20581 cgcgcctcct cgatcgaacg gaagagcggc cggtccgccg ccttgccctc gagccgcgtc
    20641 gcgatcgcct cgaacgggac gtccaggaag accgaccggc cggcccgccg gaccacggcc
    20701 cggttctctg ggaaggtgaa ggtaccgccg ccgagggcga cgacgacgtc gggaagcgct
    20761 tcgcagcccc ggaggagctc cgcctccctc tcccggaacc aggcctcgcc ccgttcctcg
    20821 aacgcgcggc ggatggtgac gccggcgagc gactcgaacg cctcgtcgag atccaggaaa
    20881 ggcaccccga gacgtccggc gagcgcgcgc cccacggtcg acttcccggc tcccatgaag
    20941 ccgacgagga agacgcgtcc gggtccggcc ttcacgacgg gagcgcggtc acgccgccgg
    21001 cgtctctccg gagagccacc gctcgagcca cttcgtcgag aagcgtccgt gccggacgtc
    21061 ggggacgtcg aggagccgca ggtggagcgg gatcgtcgtc cgcacccctt cgacgacgaa
    21121 gagctcgagg gcccgccgcc cgcgcgcgag agcctcctcg cgcgtctgtc cgtggacgat
    21181 cagcttcgcg aggagggagt cgtagtgcgg cgggatccgc cagccgatgt aggcggcggt
    21241 gtcgacgcgg acccccggtc ccccgggaac gtgaagggtc gtgatcgtgc cgggagacgg
    21301 cgtgaacttc tccgggtctt cggcgttgat ccggaactcg atcgcgtggc cgcgggggcg
    21361 gaactcgtcc gtcgcgccgc cgaccctcgg gaacgagagc ggctcacccg cggcgacccg
    21421 gatctgctcc ttcacgaggt cgaagccggt caccatctcg gtcaccgggt gctcgacctg
    21481 gatccgcgtg ttcatctcca tgaagtagaa ggagccgtcc tggtcgagga ggaactccac
    21541 cgtgccggcg cccacgtagt cgacggcgcg cgcggctgcg atcgcggccg cgcccatcct
    21601 ctcccgcagc tccggcgaca cggcggggga cggcgactcc tcgaagacct tctgatgccg
    21661 ccgctgcagc gagcattccc gctccccgag gtggacgatc tttccgagac ggtcgccgaa
    21721 gacctggacc tctacgtgcc tcggctcggc gaggtacttc tcgaggtaga gggagccgtt
    21781 cgagaacgcc ctctcggcct ccacggtcgc gagctcgaac gcggccgcga gctccttccc
    21841 gttccgggcg acgcgcatcc cgcgcccgcc accgcccgcg accgccttca ggaggaccgg
    21901 gtacccgatt cgctcggcca gcttcagcgc ctcctcggtc gagccgacgg ggccgggact
    21961 tcccgggatc gtcggcactc cggccctctt cgccgtgtcc ttcgccgtcg acttgtcgcc
    22021 catccgccgg atcgcctcgg gaggggggcc gatccaggtc aggccgacct cctcgaggat
    22081 ctcggcgaag tgcgcgttct ccgcgaggaa gccgtagccc gggtggaccg cgtccgcccc
    22141 ggtgatctcg gccgccgcga cgagcgaggt gatgttcagg tacgacgccg agggggccgc
    22201 cggcccgatg cagaccgact cgtcggcggc ctcgacgtgc agcgcgtccc ggtcgaccgt
    22261 cgagtggacg gcgacggtcg cgattccgag ctccttgcac gcggcgatga tccgcagggc
    22321 gatctccccg cggttcgcga cgaggacctt gcgaaacatc aggccggccg gatcccgaac
    22381 agcttctcgc cgaactcgac cggctggccg ttcttcggga aggcctcgac gaccgtcccg
    22441 gcgacgtcgg cctcgatctc gttcatcagc ttcatcgctt cgataatgca gagcgtcgcg
    22501 cccttctcca ccgtgtcgcc gaccttcacg aacggctcgg cttccgggtt cggggcgcga
    22561 tagaaggtcc cgacgatcgg cgaggtgacg acgtggaccc ccgagtcgac ctcgggcgcg
    22621 gccgccggcg gtgccggcgc gggggcttcg atccgtggcg ccttctcttc ccacgaaacg
    22681 gggacggccg ggtaggccga gacgggaacg ggcgccgcct cgcggaccgc gggccgcggc
    22741 ccctcgatgc gcaggcggaa gccaccttct tcgacctcga ggctggcgag gccccggcga
    22801 gcgaccagat ctgcgagttc cctgatttcc ttgacgttca tctctctctc ttcgagggat
    22861 tcgaattgcc gcggggcggg tcggccctca gaggagggcc ggacgaagct gctcggcggc
    22921 ccgcgccgcc tcgaaccgga tccgtcccac cgggacgccg gcgccggtct tcagcaggag
    22981 cgcgtactcg gtcgtgatcg cctgcatggc cgccgtgccg gcgctcccct tgaggacgag
    23041 gcttcggagc gcgccctcgc cgacgtcggc gcgggcgcgg cggacgttct tccagaatgc
    23101 cgtgagctcg gcggagaggc tctccatcgc ctcggcctcg aggcgcgatc cgacggtgac
    23161 gacggggacg ccttcgaggt cggtcacgag ggccgcggcc ggcgcgagcc gctccacgat
    23221 ctcctcgaca gccgtgacga ggtctccgtt tcccgaccgc ccgtcccggg cgaagggctc
    23281 gacccaggcc tcgagccgcc cccggaccga cgagctcgcc gtcgccgcga gctccgccgc
    23341 gacctcgaga taccggctca ccttcgccgg gtcggtctcc gtgcgggcca gagcgaggta
    23401 ggcctcgcgg gcgcgtccga gatggccctg ggcgcgcagg agcccggcca tcgtctccgt
    23461 ggcgacgggg atcggaagcg acgccgcctc cggagccgcc agcggctcct ctagggcgcc
    23521 cgcggatgcg ggaggccccg agggcgaaag ctcagccagg acttcggccc gggcgccctc
    23581 cccgaaaggc ccgggcccca tatcggcctc cggactctcg ccgtccgggg ctgtcgagga
    23641 ctccacgaga atgtcgggaa tctcctccga gggagggacc gcagggacct ccggtagaag
    23701 ctccagacgc gtcgactcgg actccacctc tgcgaagggc ggcgtgcccg gactttcggc
    23761 ctccgacgcc tccgccgccg cggcggcgtc gagcccaggt cccggctcgg cgaccggggt
    23821 cggcggcaac tcgaccgagg cggccgccgg cgcgatcggc gcggcaggcg cgtccacgag
    23881 agtgagccgg gcctctggcg gaacggatgt ctccgtcgcg ggtgccggca cgggcggaac
    23941 gggcgggacc ggcacgggaa ggcggattcc cgatctcggc gagggcgggg gcgccagctc
    24001 ggcatcgagc tgccggatca gctccgcgac ctcccggtcg cccgggctga gcccgcggaa
    24061 gagcttcagc ttcttgaccg cctcgacctt ctctccgcgg gcgaggtgga cctccgcgag
    24121 ctgccggatg gccacgaggt tctgcgggtc gagcgtcaga gccgtctgga acgaatcgac
    24181 cgccttctcg agctgctccg tctgcagtag aagccggccg agcgcgacgt gccccgcgac
    24241 cgaagcgggg ctctgctgca gcccggcttc gaggacctcg atcgcctcgc gcggtcgccc
    24301 ctgccgacga tgctcgtcgg cgacgacgag gaactggcgc ggcccggtct cccgggtcaa
    24361 gctcctcttg atctcgtcga tgcgagacag cgtcctgctg aagaggccca tcggtcccgg
    24421 ccagtgtacg gcaaagccgc cggcggaaaa agctcgggcc gccccgaagg gcggcccgga
    24481 aggggttcgg gagagcggga tgccccgcgt cagttgagag agaacgtata cgtcgcggag
    24541 gccgtgtggc tcgcgccgcc gcacgaaggc gtatcggtag cggtgaccgt gatgttgtag
    24601 gggccgccgg ccaccgccgt tccgctcgtc gaaagtaccc cggagccgtt gatcacgacc
    24661 tggttggcac ccgggccagt aaccacccag ttcacaggct ggaagtcggc gggggtcgcg
    24721 ttcggcgtca acgtgaatgc tccgatctgc gtcccggccg cggccgacac cgtcgtcgag
    24781 gccggcgccg tgatcgagaa ctgcccgttc ggatcgatga gctgcaggcc aatcgtgcga
    24841 ctcacggtcg tcgccgtccc gtcggtggcg ctgaggccga ccgagagcgt cgtcgtcccg
    24901 ccggcccccg ccgtgggcag ctgcggtgtc ccggagatgg cgccggtgcc gaggtcgatg
    24961 gacattccag gaggaagccc cgtcgcattc caggtgtagc ccggagtccc ccccgtcgcg
    25021 gtcgcggtcg cgccggcgta ggctgtgcag atcgtcgcgg cgggaagggt cagcgtcgtg
    25081 atcgtgagag gcgtccgaca gacgacgggc tcgggagggt tgtagatgag gatgttcggg
    25141 agctcgacca cgcagccggt ccgggcgttc cgaagccgga tgccgaacga cgttgggacg
    25201 tacttcacac ccgtgacgcc ggcgttcggg gcgggaatgc agggctgctc gttcagtgcc
    25261 gagtcgggga cggccggggc cgtgaagctg agctggtccg cgttcgcgac gatggcgttg
    25321 ttgatcggga tggtgacgcc gccgccgtag acctcgacgg tcatcgggtc gatgaagttg
    25381 gtcccgatca ccgtgatggt cgcgggggtg ccgccgatgg gcgtgccgaa gggacctccg
    25441 gtctggttca gctgcgtcgg ggtggcgctc accgcggtca tcgcgtcgac ccgataggtg
    25501 aacggggtgc cgagcgtgtt gcaggccgcc gaggagagcg cggggaagga gatcgtgagc
    25561 gtcccggtga cgttgtcgca ggccggtccg ccgccgagct gcgggtcgat cacgggcatg
    25621 acgagcgtga tgagggagga ggagaccgaa gtgacctgcg gacgcaggag caagccgccg
    25681 gattcgaagg tcacctcgac cggctcctcg aagccctggc cggcgatcgt cacgaccgtg
    25741 gactggttcc acggcgccga cgacgggatc acggtgttga gcgtcggaca ggcgtagtag
    25801 cggaactgga cggggctcgt gtagtccttg ccggcgtacg ggtcgcggac gacgacgtcg
    25861 accgtccggc ccgcgagggc gctgttcggc ccggtggcga gaggcgtcag gaagatgatc
    25921 tggctcgcct tgatctcgac gaccgaggcc tcgacgccgc cgacgaagac ctgcatcggg
    25981 agtttgaagt tgcggccgaa gatcgtgacg cgcgtcgagg cgtcgttcgg gcccgtggag
    26041 ggcgagatcg acgtgacgac cggctcgagg aaccctccgg ggaagtacgt gtaggccttg
    26101 acggcctggg cacatgcttc ccgcggtccg cccgcgtcga cgatgacgcg gacgtcgacc
    26161 gtctcgggct tgtcggggct ggcgaggacg cgccgcggcg tcgtcacccg gatctcggag
    26221 tcgctgacgc tcagcgtgac gccctggttg ccgccgaaga agacggccgt gcggtcggtc
    26281 gtcgtgctcg tgccgccggc cgtgaaccct ccgccctgga tgacgacgat gtctccgccg
    26341 tccgggctgc ccgcgttcgt cgggtcctcg gggaggaccg tcgcgaggaa gaggccgggg
    26401 actccgttgc aggtgccggc gccgccgtcg gcgcgatagg tgaacgcctg cggcaggaga
    26461 ccgctctgct gcgagacgag gccgaggtcg gcagtgacgg cgacgtcgac cgtctcgggg
    26521 acggcggggt tcgccaggac gtggcgcggc gtctggaccg tgatctgcgt ccccgtgacg
    26581 gagacgacgg acgcgggcgc ggagccaaac gtgacgcgcg tcgtggcgac ggcgctcgtc
    26641 ggcgacggca ggaagttcgt gcccgtgatc acgacctgct gcccgccctc gggcttgccc
    26701 gagttcgggt cgaccgacgt gagggtcagg cgcttgttcg ggtcgacgca gtagtacgtg
    26761 aacgccttct gctcggtgat gtcaccgagg ggaacgtcgc ccgagaagaa gcggacgacg
    26821 aggtccacca cctcggggac aagcgggttc gcgagcgttc gcgccggggt caggacctcg
    26881 atccgcgtct cggagagtga ctggaccgac gccggcttgc cgccgaagag gacctgcacg
    26941 ttcgctacgt cggtgccgaa ccggccgccg ttgatcgtga cgacgtcgcc gccggcgcag
    27001 ctccccgacg tcgggctgat gctggagatg aacggcccgt tcgacgggat gggctggtag
    27061 tcgaccgact tctcggccgt gccgcactcg aaggcggcct tcaccttcgc gaggcccgac
    27121 gaggtcgcgc cgagggtcac gtcggcgaac ccgttctggg tcaccttcga cacgctcgga
    27181 aggcccgtct cgaggaagaa gccgaagtcc gtcgtgaagg tgatcgaggt cccgtcgggg
    27241 acgagggccc cgccctttcg gaccgtcgcc cggaggatga tcgcggtccc cgccatcggg
    27301 gtgacgtccg tggcgtcgag agcgatcgtc accgcgcagc tgccgccgtc ggttcccccg
    27361 cccgcagggg gtttgggcga cgtgggcgac tcgccggagc agccggcgag gagcagcgag
    27421 agacccacgg tcgcggcgaa gaggggctgg agggttcggc gcatcgcttt cacctcggag
    27481 tttccacgca ccgtcgacat gccgtcctca gttcacccgc gccacgatcg tctccgtcgc
    27541 ggcgctgtag aggaaggtga ggccgaaggt gccgacgccc cggacgggct ggccggagag
    27601 cgtccggccg cggtaggtca cggtggcccg gcagcggatc tcgctgttcc ccgtctcgcg
    27661 gtcgatcccc ccgttgaagg ggaggagctg gtcgagcggg gccttgagga gagccgagcg
    27721 ggtcatatag gggtagttgg acatcgtcga cgtcccgccg gccgggacga tgacgttgcc
    27781 cgggaacgtc tccgcctccg gcgccttcgt cccgccgtcg atccgccgcc actcgacgac
    27841 gtagtcgtcg aggcgcgtgt cgaggaagct cgacgtcccc ccggtcgtgt tcttcaggtg
    27901 cgaccggagc tggaccgtct cgagctggag cggggcaccg ctcccgacgt tccacgactc
    27961 cgggaggagc gtgtactcgg cggagaggta gaccggcgag gcgtcgtccc cctgggtgtt
    28021 cgacgaacag ccggcgacgg cgaggactcc ggccaggagg gcgacggcga ggggaattcg
    28081 gttcttcatc gttcggctcc gtgtcgcggc ctcagagcgc cttcatcacg cgcggcgtga
    28141 tgaagatcat cagctcgtct gtcgtctcgt ccatcgtctt gttccggaag agacctccga
    28201 ggagggggat cttccagagg cccgggacca tgttctggcc gtcgttggcc gagagcttga
    28261 agatccccgc gatcatcccg gtgccgccgt cgcgcaccat gaggcgggtt tgcgcgtcgc
    28321 gggtgacgag cgggacgttg ttcccgccgg tgatcgcgac gcccggcgcc ggctcgcggc
    28381 gctggatctt gatgtcgagg atgacggtcc cctccgccgt gacctgtggc gtgacgtcga
    28441 gcctcagcgt ggcgtcgatg tagaggacgg tcgtcgtgtt gttgaccgtc gtctggaccg
    28501 ggatctggaa gcccgactgg accgacgcgg ccatgttcgt ctgcgtcagg atcttgggcg
    28561 acgagacgag cttcacgagg ccccgggctt cggcggcgct caacgcgacg tcgagccgga
    28621 acgtgtcgag gacgttcgcg aggctcgccc gaaggatctg attgccgttc gggagcgaga
    28681 ccgcgccctc cacctcggcg ctgttcggga agaccatccc ggtcgtgttc ccgtgagcct
    28741 ggtcggagat cgcctggaag ccccagtcga tgccgagccg cttactgaac gtccgggtcg
    28801 cctcgacgat ccgggcctcg atcatgacct gcggcacggg cgtgtcgagg ctcttgacga
    28861 ggtcgaggac cgtcgggagg tactccggga gctccttgat gatgaggagg ttcgagcgct
    28921 cgtccacgaa gatgtcgccc ctcggcgaca tgaccttctt cgccgtcgtc gccgcgctgt
    28981 tcgcgctcgc gtacgagagc ttctggatca ccgtccgggt cggacgattc gcctcctgcg
    29041 ccttgaggag gttctggcgg gcggactcct cgtgggcgag cttgccggtc gccgcgatcc
    29101 gcatgacgtt cccctcgagc acgtagccga gcccgttgat cttcaggatc agctcgaggg
    29161 cctggtccca ggggatgtcg gtcagcgaga cggtgaccgt gccgcgcacg tcgggatcga
    29221 ggacgatgtt caggttcgtc agctcgctga acttctggag cgtgtccttg atgtcggcgt
    29281 ccttgaggtt cagggtcagg ggctcgccgg tgtactggcg ctccgtctgg ccgatcgccc
    29341 gggactcgta cgggttcgag atgtccttca gctggccgcc gctctgctgc tcggcggcga
    29401 gcgtctcggc ggcctcgagg agcgcacggt cgtccgcgga ggtcttccgg cgggcggggc
    29461 gcggcggaac gggcgccggg accgcgacct cgggggaggc caccgtcacc gtcggggccg
    29521 ccgccgcgac gcggacgggc tcgggcgtct tcacggcgac cggctcggga gccggcagct
    29581 cggcgacgac gacgggctcc gcaggggccg gaggctcgac ggccgccggc tcgacgacgg
    29641 gggcggcctc ggcgacggcg gggagctccg cagctgcggg gacgtggacg accggctcgg
    29701 cgaccaccgg cgccgcggcg accacctcgg gagccggcgc ggtcgggacg atcggctcgg
    29761 cgaggtcgtc ggcctcgtga gcctcgtacg aaccggcggc cgggacggca ggcgcgggag
    29821 ccggggccgc agccgcgacc tgaacggggg cctcggcctc tccgccgaac gagatcgaga
    29881 cggtcgatcc gctctgccgg aggaccggga gggattcctt ctcgaggtcg aagacgacgc
    29941 gcgtgaccgc ggtcggctcg gtgcggaact gggaaacgcg gacccgggcg acgccggcgg
    30001 cgtccacgtc ctcgttgcgg aagcgcgacg cgttccggac gccggagagg tcgacgacga
    30061 agcggggcgg gctcgagagc gtgaaggcct cgtaggagag ctcgccgtcg gcctcgaggc
    30121 ggacggcgag agcgccgcgc cgggtctcgg tcgccacgcg cgccagacgg gtcgcgaccg
    30181 cgccggacgc ctcgtggcgg gcgacgacga caggctccga cggggacgcg gcggcgggag
    30241 cggggacggt cgggggagcg gcctgcgcga cgaccgcggg cgcggtgggc gccgcaggcg
    30301 tctcggcctg cgcgaggatc ggctcctcga tccgcgggag cagcgcgatc gacatcgccc
    30361 tggttgccgg gtccccggcg atctgggctt cgagcgcgcc ccggccggtc agctcgaacc
    30421 gcacctgcgg ctgcccgagc tccgagaacg acctcatctc gagggcggtc agaagcgtgc
    30481 cgtcggcgcg cggaggctcg agaccgggcg tcgcgaccgt tccggggagg tcgaccacga
    30541 gggtcttcga cccctcgcgg gcgtagacca tcggccgcag ggggccgtcg gccgcgagca
    30601 ggagccgcgg cgcgtctccg tcgatcagga gcgcggcgct cgtcaggacc gacggcgcga
    30661 ccgaggcgga gacgacgttc tccgtcgtcg gggcgggtcc ggcctgcgaa gagcagcccg
    30721 cggccgcgag ggccgcgacg aggattcccg cgagcccggg tgccgagaac cttccagttc
    30781 gaaacatggt tcctccccac gagaggttcg aggtgccgag gggccgaccg ggttcgaggc
    30841 gggtcgtctt gatcgtcatc gaacttcctt cgctcacggc ttcgcctgga gggccagcgc
    30901 cttgacgacg tcccggaacg gtttcgggct cgtcgggtcg ttgacgttct ggcggaacac
    30961 gatctcggtc gggccgatct ccatgacctc gccgtcgaag accgtggtcc ccttccggag
    31021 caggtacgag cggttgtcac cgcccctcat catcgcgatc cacccctgcc gggtccgaat
    31081 cgttccctgg agctccagct cctccacgag catccccgcg atcccgggcg gcctctggcg
    31141 atccttgtcg tcctgggtcc tcaggaggag ggaccggaac ggatcgcgcc ggccccggac
    31201 ctcgtaggtg taggccggcg cggtggtgtc ctgctcgccc ggcaccggga cctcgatccc
    31261 ggtctcggtc ggaggcgtcg cgggagcggc aggcggaggc gcctgtccga gcgcggacga
    31321 ggcggggagg atcgagacgg cgagaagcca ggccgacgcg cgattcagtt tcatcagacg
    31381 cctccatcgc gtcccgcacc ggacgggatc gcggctccgc cgccggcgga accgccgcgg
    31441 ggcggggtgg cccgaccgga tccggaggcc tggtcgccga ggtagatgaa cgtcttcagc
    31501 gtgaagttcg ctccgagcgt ccggccgagc ctcgcgtccg agtaccccgt catcgtcagc
    31561 gactcgacgt tgatgatccg ggagaagcgc gccatcttgt cgaagaagag cgccaggttg
    31621 tggtacgtcc cgtcgaggac cacttcgatc ggccactcgg cgtagaagtc cttgttgacg
    31681 tactccttcg ggcggaagtc cttcaggaag aagtcgccct gccgggtgag ggcctcgatc
    31741 ttcttgatga gctcctcggt gttccgcttc gtcgggagga tctcgaggag gcggttgagg
    31801 tcgagctcga tccgccggac ctcctcgcgc agctgcggga gccgccgctc ggcgacccgg
    31861 ccccgctcga tcttctcctg gagcttcccg tactcggcct tcaggccggc gagccgctgc
    31921 tgcatctcct tgaagttcgg gtactgccag ttcgcgacgg cccagagtcc gaccgcgagc
    31981 gcgaggccga tgccgaggcc gtagtaccag ggcttgtctt cgagctgctt cagttcgatc
    32041 gccacttcgg ccccctcagt tccccgcggc cggcgcggct ggagcggcgg gcgcggaggc
    32101 ggcctcgctc ccgtccggct ctgcggtcac gaactgggtc ctgtcgagat ccgcgaagac
    32161 gaaggtcaac ccgaagttgt agaggttcga ggcgccggcg ctcgcggcgg taatcgccct
    32221 gaccttcggc tcctggaacg ccgagacccg cttgaggttc gtcaggtagt tcgcgacggc
    32281 cggcggattg agggccttgc cgtcgatcgt gatgctgttg cccttcacct gcatcctctc
    32341 gatccagagg aggtccggga gggcgttgga gacctcgtcc atcaggcgga ccggcccgcg
    32401 ctggttcgcc ttgagggcgt tgatgaggtc gaccttcttc tggagcttcg ccttcttgtc
    32461 ctcgaagtcg gcgacctcct tgaggaccgc ctcgaggcgc gtgacctcct gctgggcgat
    32521 ccggttcttc tcctgctgct ccttcaggcg cgacgcctcg acccaccact ggacgccgac
    32581 gacgaggagc ccgatgagga gccccccgag gatcaggaag aggttcaggc ggcccgcgcc
    32641 gccgagcgcc gagacgccct tcttcttcct ctgtggcttc gcttcggaga ggaggttgat
    32701 cttgatcatc tggcctcctc cgtcactcgc cgagcttccg cacggcgagc ccgacgccga
    32761 tcgccagcga cgggccgttc tccgagagcc aggccggatc gacggagcgg tcctcggcga
    32821 cgacggagcg gaacggattc atgatctcga cgggcaggcc gaaccggtcc ttgaggacct
    32881 cgtcgagctg cccgacccgc gagcctccgc cggaaaggac gatggtgtcg accttttccg
    32941 agccggacgt cgcgtggaag aagtcgatcg tcttctggag ctcgccggcc acgtcctcgc
    33001 agacgcctgc gagcacgggc tggatgctct ccgcggactg cccgccgacc ggttccccct
    33061 tcttgagcgc ctcggcctgc tcgaaattga gcgagaacgc cttctgcagc gcgtccgtga
    33121 actggttccc gccgaacgtg atgtcgcgcc agaaggccgt ctgcccgctc gcgaggatgt
    33181 tcacgttcat caccgacgcg ccgatgttca ccagggcgat cacgcgcgtc gggtcgaggc
    33241 cgtagttcac ctcgtagcag ttctggagcg cgaagacgtc gacgtcgacg aggcccggcg
    33301 tccgcccggc ctgcgtgacg acgttcttgt agtcgtccac cttctccttc ttcgcggcga
    33361 cgagaagaac gcccatcgtc tcgcccggaa ccccgggatc gaggacgacg taatcgaggt
    33421 agacgtcgtt gatgtcgaac gggacgtact gctccgcctc ccaccgga
//
LOCUS       DAOONL010000031        33273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1101_length_33273_cov_19.610121, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000031 DAOONL010000000
VERSION     DAOONL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 33273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_33273_cov_19.610121"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLP50_06895"
     CDS             <1..310
                     /locus_tag="PLP50_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPA51313.1"
                     /translation="VSVVERTKEIGIRLAIGARKGDILRQFLLEATLLALGGGAVGVL
                     FGWVISSLINAFTPLPTLVTPFLITTGLLLATLTGVLAGVFPAVKASRLPPIEALRYE
                     "
     gene            303..1580
                     /locus_tag="PLP50_06900"
     CDS             303..1580
                     /locus_tag="PLP50_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51314.1"
                     /translation="MSERLFDVLRRTLGENLRFASIALRAHKLRAFLTIVGIVIGVWT
                     VIAMVALVTGFNRSTMEAFSSFGTTLVQFQKYEPRFFGGGGPPPEEERNRKDLTIEDA
                     EAIARLAPSIAAVSPERYLWGGSKVKGNGKEANSPTVAGGNQNYLVCNNWALADGRNI
                     ADVDVQHGTDVAVIGADVVKSLFEGKDPLGQPITINGRRVLVVGLLEKKGSQMGGSPD
                     NIIVIPISTFDVFFPQIKNSRRDTLHIATIPKSPELIPAAIEEGTNILRARRKVAFNE
                     PNDFAIFTAEKMIKEMTAVTNAISGVMVFIAAIALLVGGVGVMNIMLVSVTERTREIG
                     LRKSVGALRRDISLQFLTEAMTLTGLGGAVGVALGVLTALVVGAVSPLRTATPLWSVL
                     VGLGVSISIGLFFGLYPALKAARLDPIDALRWE"
     gene            complement(1663..2760)
                     /locus_tag="PLP50_06905"
     CDS             complement(1663..2760)
                     /locus_tag="PLP50_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPA51315.1"
                     /translation="MLPASLGLALVALLFRLVSPSEIRALLDLHLLSLFLVLILAVEC
                     LKRSGLIEVVVGRVLSRVRSERTLAAAAVLTTGAVSAMVTNDVALLLVVPFTLAFEKV
                     APELDPVRVVVLEIQAANLLGCLTPTGNPQNLLLFSRGGFTTASFFAAQLPWVIGMAA
                     ALLALVPILVPKRALPAPPPVQRTVEPRLATAGLFLLSLQLLAISQAVPRLVPLVAAV
                     PAAALLGRGLLKTDFTLLGVFSALFVGVEGLRRSALFETLDPVALLGATPAGFVLSGA
                     LLSQAVSNVPAAILLAPSAAAAGGGTLFTALLYGVNAGGCGTPIASLANLIGADLYLR
                     GRAHRAPFWRLFLAVSFALLAVAVALSLALA"
     gene            complement(2888..3859)
                     /gene="pip"
                     /locus_tag="PLP50_06910"
     CDS             complement(2888..3859)
                     /gene="pip"
                     /locus_tag="PLP50_06910"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HPA51316.1"
                     /translation="MAHLVKDPVSWLYPEIEPFRTGRLRVSDLHELHFEECGSPSGKP
                     VVFLHGGPGGGTSEKMRRFFHPEKYRIVLFDQRGSGKSTPYASLEENTTWDLVADTER
                     LREHLGIAKWQVFGGSWGSTLALAYAERHPERVTELVLRGIFLLRRKEIEWFYQEGAS
                     ILFPDAWEPYLAHIPEAERGDLVAAYHRRLTSDDPAVRLAAAKIWSGWEGATSKLLPD
                     ATFTGHYEEDEFALAFARIEAHYFQNRGFFEVDDQLLRDVGRIRHIPGVIVQGRYDVV
                     CPIFSAWALHRAWPEAELIVTPDSGHSAFEPGNSRALVAATDRFAAL"
     gene            complement(3988..4854)
                     /locus_tag="PLP50_06915"
     CDS             complement(3988..4854)
                     /locus_tag="PLP50_06915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPA51317.1"
                     /translation="MSGLLPGPVLAGALALLASPAFGERPPIPHVPVPAADADAPVVD
                     VLPGGVVRWPGKGIVLCGDEHETWKPRDGACYYPVDLDRGEALFPVFRDRAGARETAS
                     VRVLPPPYATESLRVDPRTVRVSEKVRRRAARERRQVVPLFSLRTNPSFSLPLAPPLA
                     EAPPARNFGTRRLFNGEPRSAHGGVDYRAKVGTPVLATEGGLVVLAAHHFFAGKNVFV
                     DHGAGLLSMYMHLSRIDVKAGQRVARGERLGLSGATGRVTGPHLHFGFRWRGARVDPN
                     LLLGDPATLPSP"
     gene            complement(4851..6500)
                     /locus_tag="PLP50_06920"
     CDS             complement(4851..6500)
                     /locus_tag="PLP50_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA51318.1"
                     /translation="MSSQDADDVKQRTVPQADRRRRLLSSERATETFARGGRHRVALG
                     FPNSYEVGMSNLGFQWVYRLLNREEDVSCERFFYDETELGGRGPRTLEGEQPLASFPL
                     VAFSVSWEMDYVHFLRMLPAARIPLLREDRGGDDPIVLVGGDCARINPLPLTPYVDAF
                     AMGDGERLVPGIAATLRADLPRRAALERLAGLKGLYVPAVHGERAEAADDGKVVVDQL
                     RAADGPGREPPHTTVLTPHTELSDKLLIEVARGCSEMCRFCWAAYAMAPQVRVPASSI
                     LAVAERCRPLTSRVGLIATAVADHPEILPILRGLSGMGFHIALSSIKIDAISEELLDV
                     LARQGEKSLAIAPEAGNERLRRAINKKVTDEMLREKVRLVARAGFANLKLYLQVGLPG
                     ETEEDVDDLVRMVDDLRLVMLEEGRKRGRLGTLVPSVNAFIPKPHTPFETEPLEDPDE
                     LARKLKKLEGAFRAMPNVTFRGMPVAEAIWEAWLAKMGLESGPILAEAAAGAPVRRLV
                     REHRETLLSVARPAARADAARRGVFDLAARQASPWGFIAKS"
     gene            6637..7077
                     /locus_tag="PLP50_06925"
     CDS             6637..7077
                     /locus_tag="PLP50_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HPA51319.1"
                     /translation="MYYVIGADGQQYGPIDEATVRGWLGEGRVNATSLSFKTGEAQWI
                     PLRDRPELGDVLAAPVAAAPPYAPPAGPMPVTPDAPKDWLVTLLLSIFLGSFGVDRFY
                     LGHVGLGIGKLLTFGGCGIWWLIDVILIALGNVTDAKGRPLVKS"
     gene            complement(7067..8092)
                     /locus_tag="PLP50_06930"
     CDS             complement(7067..8092)
                     /locus_tag="PLP50_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA51320.1"
                     /translation="MTHEEPLPPFRPAPALGQRHVQTFWGRLTRPRLLVPLRREKLAT
                     PDGDELVLDRLDGPSRAPLVVLLHGLEGSAHSVYVQGLLALLARRSLRAVALNFRSCA
                     RDLPSLRRNLPNRGRRLYHSGETADLDHLLRTLAAAEPGIALGAIGVSLGGNVLLKWL
                     GEHPGERLVAAASTISVPFDLEAGARELEGGLGPVYTATFLRGLRTKARQLLRRHPDL
                     ASRIDLGRLRRARTFFEFDDAVTGPLHGFRGAKHYYDESSAIGWVGRIATPTLCLSAE
                     DDPFLPREALDRVAARASGAVRLVRTRQGGHTGFISGASPFRVRYWAEERAAEFVAAR
                     LASPLSS"
     gene            8165..9316
                     /locus_tag="PLP50_06935"
     CDS             8165..9316
                     /locus_tag="PLP50_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPA51321.1"
                     /translation="MPRPPRLAPIPLSMKGSVYSAVLDRLAACPGETYPLHVGDTYLE
                     PATSCRMEDLAVAEHPGMHRYAPPQGIPALVDAVVERLRARTGVPLERANVFVAGGGT
                     SGLACVVGAIVAPEDEVAILAPHWPLIEGHVRMAGGVPVDVPFFGAVDSRESAVAAVE
                     TALTPRTVALYVNTPSNPTGHVVPRAWLEGLVDLARRHDLWLLFDEVYEDYVYEGEHV
                     PGLSLAPERSFAVHSCSKAYGMAGNRVGWVAGPAAPVAEALKLSTHTVYAAPTAAQLA
                     ALRVLRGPGDAWVEKTRAAYASAGAAAAERLGVPRPHGSTFLFLDVAPRLDGRGLTGF
                     LEDAADRGLAVAPGPSFGPYPTHVRVCYTSAPPDVVRRGIEVLAGLVGR"
     gene            9367..11292
                     /locus_tag="PLP50_06940"
     CDS             9367..11292
                     /locus_tag="PLP50_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51322.1"
                     /translation="MASERVRGAGAVPADRGTALLVVDDNEVNRDLLSRRLVAAGFAV
                     AGAASGPEALTALDEGRFDLVLLDVMMPEMDGLEVLRRIRARFGVGELPVVMASALGD
                     SRSLVEALRLGANDYVTKPLDMQVVLARLGTQLALRRATEDVTRLAAQLRAAQDQIST
                     LLSTGGEAFADFGRWARSMSSGVAGTLGAREISVFLLQEEAFVSVEGTTAEAPSPSAV
                     ALLPAGDGLSVEGERAVAPVRGPRGDLLGAVVADGKGEWDDVERGLLLGFAHQLGGAL
                     ELQRMKEQLAAARQRSSRLGGTTQSLEILYVCPTCSRCWGPEVRACGADGTPLTEPRG
                     FPYRVLDRYALRFLLGEGGMGAVFEAYDERLDRVVALKVVKPEHFRNATVRRRFVQEA
                     NAIAAIRHPNVVELHDSGDLGDGSMFLVMERLHGRDLADLHRFSGAGTPAQVASLLRQ
                     GAAALGAAHGAGIVHRDVKPENVFLSPARDGFRVRVLDFGLAKRVDGDRHLTQSGLIV
                     GTPAWMPPEQISGREVDERADVYSFAAVCYLALTGRGVTVERDLDKVFVDVVRNPPPP
                     VSALVPAAPAAVDAAFRAALAKKPEARPRDVVAWAAALAAALEGLPSTGPGWVFPAGG
                     GPPGSPSAPTLVTRPPA"
     assembly_gap    11344..11443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11592..11957
                     /locus_tag="PLP50_06945"
     CDS             11592..11957
                     /locus_tag="PLP50_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPA51323.1"
                     /translation="MHVEVRKKGDVVVADLTGRLVGGVGDEILREVVNELLAEGWKKI
                     LLNLSGVQVVDSAGIGELVASHKLCNKLGAQLRLLRLPKRVKETLHLAALLPVFHVFE
                     DEKDALHDLAAPAEPSGRG"
     gene            complement(11978..15163)
                     /locus_tag="PLP50_06950"
     CDS             complement(11978..15163)
                     /locus_tag="PLP50_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPA51324.1"
                     /translation="MRDAARFAPAFVFFLLAPVTAPALAAPVDSKAAVGKPPVENRFD
                     GMRFRNIGPFRGGRVTAVSGIRGQRNVHFQGATGGGVWRTTDGGTSWEPVSDEFFRTG
                     SVGAVAVAESDPNVVWVGMGEAPIRGNVSHGDGVWRSTDAGRTWKHLGLEATRQIAAL
                     RVHPKDPDTAWVAAQGKLWGPSEERGVYRTRDGGKTWARVLFVDTATGASDLALDPTN
                     PRILYAGMWQVVRRPWELVSGGPGSGLYRSEDGGDTWTRLTKGLPEGIWGKVGVAASG
                     AKPGRVFAMVEAEKGGLFRSEDWGESFTKVNEENDLRQRAWYYTGVWADPKNADTVWV
                     TNVQLWRSLDGGKSFTALPTPHGDHHDLWIDPDDSSHVLVGDDGGAYVTYDAGKSWSS
                     IDNQPTGQFYRVAVDDRFPYHVYGAQQDNTTVAIASRSRGREIGRAEWHPVGGCESGW
                     IAPKPGEPDVVFAGCYGGSITRYDHRTGEEREVTAWPQLAIGRPASDLKYRIQWNAPI
                     LVSRHDPSVLWHAAQVLLESRDEGQSWKEVSPDLTRNDKAKQGSSGGPITKDNTGVEV
                     YGTIFALAESPLAKGVLWAGSDDGLVHVTRDGGKSWRNVTPKGLPEWIQVNSIEASPH
                     DAATAYLAATLYKLGDLTPHLWKTADYGASWTRIDAGIARDAFTRVVREDPVRKGLLF
                     AGTETGLWMSLDDGRGWQRFQRNLPAVPITDLAVKDGDLVVATQGRAFWILDDLSPLR
                     QWTEEVAAAKTWLFTPPAAFRLRGGGDFAKAPQGAGQNAPAGALVSFWLKESPRESDV
                     VTLAVLDGGKLLRYFSNEKRDEKLAAEAEEPLENRLELSAGLNRFAWDLRALGPTLVP
                     KAVLWGPSTGPRILPGTYTVRLTAFGETRTVPLEVLPNPAVKQTKEDLAKQAAFLGEL
                     YDTLSHVHGTVRNLRDAREQISTLAERAKRSRVRSSVLDAAKEVDARLLAVEEKLVNP
                     KLKSPQDVLNFVPALDHQVVGLISAASGADAPPTAGALAYWAELKKTVDGALRDATRT
                     LIDGVAAWNARLADAGVPPVVIPARRIPTPR"
     gene            15291..15461
                     /locus_tag="PLP50_06955"
     CDS             15291..15461
                     /locus_tag="PLP50_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51325.1"
                     /translation="MNGLATRPRTGEELDRRIRMEWAVRQVQRGFKDVQEAALAARVP
                     VTHLASVVGADA"
     gene            complement(15565..18267)
                     /locus_tag="PLP50_06960"
     CDS             complement(15565..18267)
                     /locus_tag="PLP50_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA51326.1"
                     /translation="MTCSPFRPRLARLLLASALAVPMAASAAAPAPKGLPPLLDRELF
                     FGNPEIAGAQLSPDGKWTAFLKPWKETRNVWVKKTGDPYDKAHLVTAEPKRPVAGFFW
                     SRDSKYILFVKDNDGDENYNVWAVDPAAKAEAGKDAPPARNLSDAKGVRAQIYAVPKM
                     DPDTVFVGLNDRDAAWHDLYKVKISTGERTLVKKNTEKISGWVFDLDGQLRLAARIAD
                     NGDTEILRVDTEGLVKVYSCSVFESCGPVRFHKDGKRVYMESNKGEPDLTRLILFDPA
                     TGKEEPVETDPKGRVDFGNAVFSEATDELVATAYEDEKTRIYFRDKAWEADYRLLEKK
                     LPGREVIPAGSTADDQLWMVTAYSDVDPGTRYLFDRKTKKLSVEYVSRERLPREHMAE
                     MKPISYPSSDGLVIPAYLTVPKGVPAKGLPLVVFPHGGPWARDGWGFNNYAQWFANRG
                     YAVLAPNFRGSTGYGKKFLNAGNKEWGLKMQDDITWGVKHLVKEGIADPKKVAIMGGS
                     YGGYATLAGLTFTPDLYAAGVSLVGPSNLLTLLETIPPYWEAIRIIFHERMGNPNTPE
                     GKKLLEAASPLNSAAKIVKPLLVIQGANDPRVKKAESDQIVVALRDRGFPVEYICAPD
                     EGHGFARPVNNMAAMAASEAFLAKHLGGRFQEGGTPEVVARLKEITVDPKTVTLAKKV
                     DAAAVGAPKPVAEPVPGTYAYAGTISAGGQSMQVSATRVIKADGETWVVSDTAKMPMG
                     EATDVTTLAKGTLAPVKRSIRQGPVTIDLAFADGKATGTMAMGAEPKPISADLGGPLF
                     ADGNATYDSIACLPLAAGYAATYRNFDVQKQKATLRQLKVAGTEEVKVAAGTFSAWKV
                     ELTSAEGEPGSTTLWVDSASRKVVKTSTTLPQMGGAVVTSELQP"
     gene            complement(18326..19642)
                     /locus_tag="PLP50_06965"
     CDS             complement(18326..19642)
                     /locus_tag="PLP50_06965"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51327.1"
                     /translation="MTASARAPRVVVVGGGFGGLSAVRGLRGAPVEVTLVDRRNFHLF
                     QPLLYQVATGGLSPANIATPLRALFKRQKNVRVLLGEATRVDVAGRRLLLADGELSYD
                     VLVVATGARHSYFGRPEWEELAPGLKTLEDATRIRARVLAAFEEAEREEEPARRREAL
                     TFVVVGAGPTGVELAGALAEIARHTLRDEFRRIDPAEARVLLVEGTERVLGSFAPALS
                     ARAARDLARLGIELRTGSLVTGVGPRGVTLRRGDVVEEVPARTVLWAAGVEGSPLGRL
                     VARAAGAEVDRAGRVVVEPDLTVPRRPELFVIGDLALVKPTSGRPLPGVAPVAMQQGR
                     HVARTIRARLEGRPGRPFRYVDKGSLATIGRASAVAEIGGLRFGGYPAWLAWLFVHLL
                     YIVQFGNRVLVLVQWAWSYLTWARSARLITGTNAETPSPNGTSDAP"
     gene            complement(19639..22767)
                     /locus_tag="PLP50_06970"
     CDS             complement(19639..22767)
                     /locus_tag="PLP50_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51328.1"
                     /translation="MRPALPTRRSGRRLRRAAGAALLLGALPLAAAPPRGRVEPGFGV
                     TPGGLVWVVEPAARRLVLRETGGSVIGAFPMAGDEGFVLGATDGGARVTAPTPDGLRR
                     LPSRTASEAHVEARFVFRGPDGSVRATARTTALRGSEPAFLGDEVWLVRRETGSFRLF
                     RVGPDGEAPAGSIPEADVRRLAGRVSSPRVFGGSSAAVLFPGARAEVLWTAATGLVVP
                     DPLEACGEGRSRFLYRHAEGLARGSIRTAPSADSSWGGEPLAVAELFDAQGRLLRSAP
                     LGAWAELFPLPDGGLLGLDGMEAVRFDDRFAEISRAVLPLEEGSDTEAATRVVEQLRR
                     LERLGARASGADWAELALLPGAPAGSHVALAHRDPGGALARLSRVSDADASAPLAERA
                     VALLVDLAETEERSGLRRDLRALVEAGGPVWLRRAVAFVLLDGPGEAPAWALPAVAEA
                     VASGAAGDSLELPDEAWTTELAELVTAVDRARIDRIAAERPELAEALLAGSLDDAFSS
                     SFEELRFHAPARLFARTLLDCAAGPPSVAGLLALTRVAEAAIEAAPPPAEAHFGRSDE
                     AKGSSIRGALAATLLEAFRSPEPSLRAGALALGPLTGLPLDAGLFRSEVLRRPHLGAL
                     ATLGLLSDRSLSPRDWVGLFAELLAVARAASADPSACSVSAWPVLQAEGDGNRDRYCN
                     LFAIVRFAALDLGGDDDPAVISRERVVLLAELARSSAAPPELRAELKLSRALRGTASE
                     EEVTELLDEKGLAFAYRRLALEKARPPAPRLAAHLERELASGRVPPAERGTWIDALAR
                     LDPAAADRVAAEAWARGNVPLDGDDGEAGAFCRALDADRVRQSEPLRAALRAARTRPA
                     ASFEAASLLAKAGDAGSAGPLANALLERCPACVSSSELAALFGPLGEAGRSELARVAE
                     AVLPFGVSPLEALFEVDPARAEALALDHLDAALERGCVPEPLVAALLGNGLDPFRALL
                     SALEERGCDRARLRPGEPVAVGLARPAGTESGDAARDALDAAPTELCRRALARLLGVE
                     DDELLPDRPEGR"
     gene            22875..24500
                     /gene="aceB"
                     /locus_tag="PLP50_06975"
     CDS             22875..24500
                     /gene="aceB"
                     /locus_tag="PLP50_06975"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HPA51329.1"
                     /translation="MPESNVLSVLASEGIEVLGPIGPLYDEILTPEALRFVASLQRAF
                     GARREELLERRVEVQRRIDAGVLPDFLPETEEIRKGGWAVAPIPPMLLDRRVEITGPV
                     DRKMVINALNSGASVFLADFEDANSPTWANCVEGQLNLRDAVDGTIGYRAPDSGKEYR
                     LAEKTAVLMVRPRGWHLLEKHLLVDGRPCSGSLFDFGLFLFHGGRRLIAQGKTPAFYL
                     PKLEHHLEARLWNDVFTKAQKDLGVPHGTIRATVLIETILAAFQMDEILWELRDHSAG
                     LNCGRWDYIFSFIKRLGRLPGYVLPDRGLVTMEKRFLASYVTLLIQTCHRRGIHAMGG
                     MAAQIPIKNDPAANEAALAKVRADKLREVKAGHDGTWVAHPGLVPIAMEVFDAGMPQP
                     NQIATAMPAAEITAKDLLAVPEGPITEKGLRQNLNVGILYLEAWLRGNGCVPLYNLME
                     DAATSEISRTQVWQWLKNRVTLADGRTLTPELYGQVFDEEVGAVRARLAEDGVGSGRY
                     ELAARLFDEMIRSEELPEFLTLSAYEELMKMGA"
     gene            complement(24590..25645)
                     /gene="gap"
                     /locus_tag="PLP50_06980"
     CDS             complement(24590..25645)
                     /gene="gap"
                     /locus_tag="PLP50_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPA51330.1"
                     /translation="MATKVGINGFGRIGRQVLKAIRDKYPTELEVVAVNDIGDLKTMA
                     HLLKYDSTYGRFDGTVEVAGEDLLVDGKHLKMLKETDPAALPWKDLGVDIVIESTGLF
                     TIKSDGVNKKGKAVKGAENHITKGGAKKVIISAPAEGEDLTIVLGVNEKSYDPAKHHV
                     VSNASCTTNCLAPAAKVVHDRWTIQRGFMTTIHAYTNDQRILDLPHSDLRRARAAGLS
                     IIPTTTGAAKAVALVIPDLKGKFDGYSLRVPTPTVSVVDFTAQLATPTTTDELRQAFR
                     DAAAGAMKGILAAVDEPLVSVDFKGDPHSSSVDLPFTMVLGKEKSDFVKVVTWYDNEW
                     GYSMRTADLAALMAKTL"
     gene            complement(25731..26285)
                     /locus_tag="PLP50_06985"
     CDS             complement(25731..26285)
                     /locus_tag="PLP50_06985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA51331.1"
                     /translation="MARPAPRSASEPSPRATLTLEEDGARHTFHLDHAVTVGRDPACD
                     VLLSSPTVSRRHALVFPDGEGWTVRDLGSGNGTFLDARRVDEAPLPNGVAIRFGSVPA
                     WFEEEPQEPLTPSQKLQRALTQTLSIQPAKRARKKAAVAALAGGVALLLGATFWHRGC
                     SARGAGPDGPAEATAPIASTERPG"
     gene            complement(26289..27683)
                     /locus_tag="PLP50_06990"
     CDS             complement(26289..27683)
                     /locus_tag="PLP50_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPA51332.1"
                     /translation="MDPRSFLRALYRAAVSAVEPGRLVSEALSRRGSAVVVRSVEDPG
                     RREFVFVPRRVVLLAVGKAAPAMATAALRAIGNLVNEAVVVAPAGVPPEELPSGGRYL
                     PSSHPVPDERSLTAGREVLSLAAGLGRDDLLVVLLSGGGSSLLALPPEEVPLADKVRT
                     VSLLAAAGAPVEDVNLVRGALSRLKGGRLAAAARQADVVTLVLSDLGDAGWHLVASGP
                     TLGVPPSRRTAATLLETWRILPLVPPSVRSFLATPPAHEEVRDGGQRWGVLLGDVRTA
                     LDGARREALRLGADVRVLPELLSGEARAAARRLAVAGASAGNLVRRAGAPPRRLVTLF
                     GGETTVTVKGRGQGGRCRELALATAFHVDGIAGATLLVAGTDGVDHTPDAAGAFADHA
                     TLARAAALGLDPTVALTRNDSGPFFAALGDAFAPGPTGTAVGDVAFVLAPGGEDVPSA
                     LDLPEEGIPLPDRI"
     gene            27765..28055
                     /locus_tag="PLP50_06995"
     CDS             27765..28055
                     /locus_tag="PLP50_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51333.1"
                     /translation="MPQVDVDRIVGSIREGAGAVREKTQDAVEDVADKIEDALEKAGT
                     EGRKLQREVKEELARRWRQVDRVGRENAFLMAAGALAVGVLVGWLISRDRRS"
     gene            28063..29313
                     /locus_tag="PLP50_07000"
     CDS             28063..29313
                     /locus_tag="PLP50_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51334.1"
                     /translation="MDLAARFSELKSGFARSFWVANVLELFERFSFYGSKIVLAVFLA
                     ETVGLGPFGVSLVGLYGFAVYFLPILAGPVVDRFGFKKSLAACFAIFSLGYFLIGLSG
                     MPMGQPLVSAVGTKTWVVCALLITAVGGSLIKPCIVGTVARTTTKDTKALGYSIYYTL
                     VNIGGWLGPVLASQVRVSLGIAQVLVGASLVSFLLFLGTLVFFKEPARDPGAEERTLG
                     KVLKDAALVLANGRFIGFLVIFTGFWVMFWQVYDLFPFYVRDVLKVERFELIGSLEAF
                     SVIFLTVPVAALMKKVRAVPQMTLGLAVGSCSWLLLVVSQTWQAAAAAMFLLALGEVL
                     QAPRYYEYIADLAPKEQVGTFMGFAFLPIALGALLAGPLGGVLLQSYLKETMQPAKAW
                     MILSGLGFVSTAALLAYDRFLVRR"
     gene            29421..30248
                     /locus_tag="PLP50_07005"
     CDS             29421..30248
                     /locus_tag="PLP50_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51335.1"
                     /translation="MSYFLSTLLLLMGVQSMYQKFYAARSPKDAKTAVAIWIVGTIVV
                     ESIVIAIAVYASSYNAEHNMGWDPRSLVLNAARHMVPGPVGILLLGAACAVVLSTGMN
                     YLLSSSSNVMRDIYQRFVNTDADPTKMVALQKVFVLALGVLAFLLIFVPTYFKLQISV
                     LQYAYFAYTIYGVAVTPALFAALTWKRATRQGGLASIVVGTLATVFFEVVLPRIAPGV
                     IEGAKGAGAAYFGGDPWGIPSIYPAAILSIGALVVVSLLTPPPTEEQLAPLFGEKKM"
     gene            30338..31258
                     /locus_tag="PLP50_07010"
     CDS             30338..31258
                     /locus_tag="PLP50_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51336.1"
                     /translation="MGLGFLYESPFFREPFPFSLPTFLALELLVVTGLTAFRLAVLGY
                     SLAEAASLLPLDAAVAAVLLLATLGNPLKAPLVYGVAYILFVVAFLRQEPPSGFWRWF
                     GTFVVLGLLWGVFALLWRISPLVTGALLLSYILVRVLLPLHYRNEAREVLAKGKPFPV
                     GQRFVATLADHGSFSLAREKFRWFLDDGARSGEGTRAVRAAHRQVRARMKELGVYHVA
                     WDRLHARTERLQDGRELYLYEGRTVGLLRRKEDLPAGGRLENTAAAEESQLPALLLDV
                     GPIAVRLVASPGGGYRVDLPPPEIPLRLVP"
     gene            complement(31333..32748)
                     /locus_tag="PLP50_07015"
     CDS             complement(31333..32748)
                     /locus_tag="PLP50_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA51337.1"
                     /translation="MSAPGPRGLPVLVCDDAAGIRKTLAQILGDEGYRVETFEDGGSV
                     LSRLGERTGPRPAALFLDVWLPDVDGLSVLDQLRARGHDLPVVMISGHGTVETAVQAV
                     KRGADDFLEKPLALERVLLTLEKALERSRLARERDVLAGEVARQRERLEEEETLLGEG
                     PAMTRLREELRRAGASDARVLITGESGVGKEIAARTLHRLSPRAGGPFVEVNGAAIPE
                     DLIESELFGHVKGSFTGATEDRKGKWEAADGGTLFLDEIGDMSPRTQAKILRAIQDGK
                     ISRVGGTRTIETDVRIVAATNRDLPKMIAAGTFREDLYFRLAVVPIRVPTLAERTEDI
                     PVLVAHFAGKLARRRGRRPPTFSPEALEELKRRPWPGNVRELQNVVERALLLADGAVI
                     GPEDLPGDVLRLPRTATGENDGLLPGQTLADARDAFERRVVVRVLEECRGNVSRAAER
                     LGLDRTTLHRRLRAWGLTDEK"
     gene            complement(32771..>33273)
                     /locus_tag="PLP50_07020"
     CDS             complement(32771..>33273)
                     /locus_tag="PLP50_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA51338.1"
                     /translation="EVRSLAALVDAFHRFARLPETRLGELDLGAVVAQVVKLYEGTKA
                     GVRVAAETAPDLPAVRGDAEQVKRALVNLVDNAVAATPPGGAVSVRASVDRGLARVVV
                     ADDGPGIPPEERDLVFEPTFSTKASGYGLGLSITSRIAAEHRGRVILEENAPRGCRFV
                     FEWPAA"
BASE COUNT         4512 a        11872 c        12146 g         4643 t
ORIGIN      
        1 cgtctcggtc gtcgagcgga cgaaggagat cggcatccgc ctcgcgatcg gggcgcgcaa
       61 gggcgacatc ctccggcagt tcctcctcga ggcgacgctc ctcgcgctcg gcggaggggc
      121 ggtcggcgtc ctgttcggct gggtcatctc gtcgctcatc aacgccttta cgcccctgcc
      181 gaccctcgtg acgcccttcc tcatcacgac cggcctcctc ctcgccacgc tcaccggcgt
      241 cctcgccggc gtcttcccgg cggtcaaggc ctcgcgcctg ccgccgatcg aggccctgcg
      301 ctatgagtga gcgcctcttc gacgtcctcc ggcggactct cggcgagaac ctccgcttcg
      361 cctcgatcgc tcttcgcgcc cacaagctcc gcgcgttcct gacgatcgtc gggatcgtca
      421 tcggtgtctg gaccgtcatc gcgatggtcg ccctcgtcac cggcttcaac cgctcgacga
      481 tggaggcgtt ctcctccttc ggcacgacgc tcgtccagtt ccagaagtac gagccgcgct
      541 tcttcggcgg cggcggcccc cctcccgagg aggagcggaa ccggaaggac ctgacgatcg
      601 aggacgccga ggcgatcgcc cgcctcgccc cctccatcgc ggccgtctcg cccgagcgct
      661 acctgtgggg cgggtcgaag gtgaagggga acggcaagga ggcgaactcg ccgaccgtgg
      721 cgggcgggaa ccagaactac ctcgtctgca acaactgggc gctcgccgac ggacggaaca
      781 tcgcggacgt cgacgtccag cacggcaccg acgtcgccgt catcggggcc gacgtcgtca
      841 agtcgctctt cgaggggaag gacccgctcg gccagccgat tacgatcaac ggccgaagag
      901 tcctcgtcgt cgggctcctc gagaagaagg gctcgcagat gggcggcagc cccgacaaca
      961 tcatcgtcat cccgatctcg acgttcgacg tcttcttccc gcagatcaag aactcccggc
     1021 gcgacaccct ccacatcgcg acgatcccga agtcgcccga gctgatcccg gcggcgatcg
     1081 aggaggggac gaacatcctc cgcgcccggc gcaaggtcgc gttcaacgag ccgaacgact
     1141 tcgccatctt cacggcggag aagatgatca aggagatgac cgcggtgacg aacgcgatct
     1201 ccggggtcat ggtcttcatc gccgcgatcg cgctcctcgt cggcggcgtc ggagtcatga
     1261 acatcatgct cgtctcggtg acggagcgga cgcgcgagat cggcctccgc aagtcggtcg
     1321 gcgccctccg gcgcgacatc agcctccagt tcctcacgga ggcgatgacg ctcacgggcc
     1381 tcggcggcgc ggtcggcgtc gcgctcggcg tcctgacggc cctcgtcgtc ggcgccgtct
     1441 cgccgctccg aaccgcgacc ccgctctgga gcgtcctcgt cggcctcggg gtcagcatct
     1501 ccatcggcct cttcttcggc ctctaccccg ccctcaaggc cgcccgcctc gacccgatcg
     1561 acgccctccg ctgggagtaa gaccgagggg ggggggtcag gtctaccatc tacactctac
     1621 gggtacccgt agagtgtaga tggtagacct gaccccccct ctctaggcga gggcgaggga
     1681 gagcgcgacc gcgacggcga ggagcgcgaa ggagaccgcg aggaagaggc gccagaacgg
     1741 ggcgcggtgg gcgcggccgc ggaggtagag gtcggcgccg atcaggttcg cgagggaggc
     1801 gatgggcgtg ccgcagcctc cggcgttcac gccgtagagg agcgcggtga agagcgttcc
     1861 ccctccggcc gccgccgccg aaggggcgag gaggatcgcg gcggggacgt tcgagacggc
     1921 ctgcgagagg agcgcgcccg agaggacgaa gcccgcgggg gtggctccca ggagagcgac
     1981 cgggtcgagc gtttcgaaga gcgcggagcg gcgcagcccc tccacgccga cgaagagcgc
     2041 cgagaagacg ccgaggagcg tgaagtcggt cttcaggagc cctcgcccca ggagcgccgc
     2101 ggccgggacg gccgccacga gcgggacgag ccgagggacg gcctgggaga tcgcgaggag
     2161 ctgcagcgag aggaggaaga ggccggccgt ggcgaggcgc ggctcgaccg tcctctgcac
     2221 cgggggcggg gcggggagcg cccgtttcgg caccaggatc gggacgagcg cgagcagtgc
     2281 ggcggccatt ccgatcaccc acggcagctg ggccgcgaag aacgacgcgg tcgtgaagcc
     2341 gccgcgggag aagaggagga ggttctgcgg gttcccggtc ggcgtcaggc agccgaggag
     2401 gttcgccgcc tggatctcca ggacgacgac ccggacgggg tcgagctcgg gagcgacctt
     2461 ctcgaacgcg agcgtgaacg ggacgacgag gagcagggcg acgtcattcg tcaccatcgc
     2521 cgagacggcg ccggtcgtca ggaccgccgc ggcggcgagc gtccgctccg agcggaccct
     2581 cgataggacc cgcccgacga cgacctcgat caggcccgag cgcttcaggc actccacggc
     2641 gaggatcagg acgaggaaga gcgagagcag gtgcaggtcg agaagcgccc ggatctccga
     2701 cggcgagacg agccggaaga ggagcgcgac gagcgccagc ccgagcgacg ccgggagcac
     2761 gccccatgat aaccggccgt ggggtctggt ctacactcta cactctgctc gcgaccgcgg
     2821 gcggtcccgg accgcccgcg gtcggggcac cgccccctct ctgggaggca ggaagggccc
     2881 gccggggtca gagcgcggcg aagcgatccg tcgcggcgac gagcgcgcgg ctgttgcccg
     2941 gctcgaaggc gctgtgcccc gagtcgggcg tcacgatcag ctccgcctcg ggccaggcgc
     3001 ggtgcagcgc ccaggcgctg aagatcgggc agacgacgtc gtagcgcccc tggacgatga
     3061 cgccggggat gtggcggatg cggccgacgt cgcgcaggag ctggtcgtcc acctcgaaga
     3121 agccgcggtt ctggaagtag tgcgcctcga tccgcgcgaa cgcgagcgcg aactcgtcct
     3181 cctcgtaatg cccggtgaac gtggcgtcgg ggaggagctt cgacgtcgcc ccctcccagc
     3241 cgctccagat cttcgcagcg gcgaggcgga cggccgggtc gtcgctcgtc aggcggcggt
     3301 ggtaggccgc gacgaggtcg ccgcgctccg cctcggggat gtgcgcgagg tacggctccc
     3361 aggcgtcggg gaagaggatc gacgctccct cctggtagaa ccactcgatc tccttccgcc
     3421 ggagaaggaa gatcccgcgg aggacgagct cggtgacgcg ctcgggatgg cgctcggcat
     3481 acgcgagcgc gagcgtggag ccccaggagc cgccgaagac ctgccacttc gcgatgccga
     3541 gatgctcgcg gagcctctcg gtgtcggcca ccaggtccca cgtcgtgttc tcctcgaggc
     3601 tcgcgtaggg cgtgctcttg ccgctgccgc gctgatcgaa gaggacgatc cggtacttct
     3661 cggggtggaa gaagcgccgc atcttctcgc tcgtcccgcc ccccggcccc ccgtgcagga
     3721 agacgaccgg cttgccagac gggctaccgc actcctcgaa gtggagctcg tgcaggtcgg
     3781 agacgcggag ccgcccggtc cggaacggct cgatctcggg atacagccag ctgacggggt
     3841 ccttcacgag gtgggccatc ggaacctccg cgcgcatcct gacagatccg ggcccggagc
     3901 ggcctcggcc gagaaccgcc acggcagcgc cataacaata catttgtgca gacctgaccc
     3961 cagttcaggg gctcgcgccc gggtgggtta cggcgagggg agcgtcgcgg ggtcgccgag
     4021 gaggaggttc gggtcgacgc gggcgccgcg ccagcggaag ccgaagtgga ggtgcgggcc
     4081 ggtgacgcgg ccggtcgcgc ccgagaggcc gaggcgctcg ccgcgcgcga cgcgctggcc
     4141 ggccttcacg tcgatccgcg agaggtgcat gtacatcgag aggaggccgg cgccgtggtc
     4201 gacgaagacg ttcttcccgg cgaagaagtg atgggccgcg aggacgacga gcccgccctc
     4261 ggtcgcgagg accggcgtcc cgaccttcgc ccggtagtcg acgccgccgt gcgcggagcg
     4321 gggctcgccg ttgaagaggc gccgcgtccc gaagttgcgc gcgggtggcg cctcggcgag
     4381 cggcggcgcc agcgggaggg agaacgacgg gttcgtccgg agggagaaga gggggacgac
     4441 ctggcggcgc tcgcgggcgg cgcggcgccg gaccttctcc gagacccgaa cggtccgcgg
     4501 gtcgacacgg aggctttcgg tcgcgtaggg cggcggaagc acgcggaccg acgcggtctc
     4561 ccgcgctccc gcgcggtcgc gaaagacggg gaagagcgcc tcgccccggt cgaggtcgac
     4621 cgggtagtag caggcgccgt cgcgcggctt ccaggtctcg tgctcgtccc cgcagagaac
     4681 gatccccttg cccggccagc ggacgacgcc tcccgggagg acgtcgacga cgggcgcgtc
     4741 cgcatcggcc gcggggaccg ggacgtgcgg aatcgggggt cgctcgccga acgccgggga
     4801 ggcgaggagc gcgagggcgc cggcgagaac ggggccgggg agaaggccgc tcatgacttc
     4861 gcgatgaagc cccacggcga ggcctgccgg gccgcgaggt cgaagacccc gcgacgggcc
     4921 gcgtcggcgc gggccgcggg ccgcgccacg gagaggagcg tctcgcggtg ctcccgcacg
     4981 agccgccgga cgggcgcccc ggccgcggcc tccgccagga tcggcccgga ctcgagcccc
     5041 atcttcgcga gccaggcctc ccagatcgcc tccgcgacgg gcatcccgcg gaacgtcacg
     5101 ttcggcatcg cgcggaaggc cccctcgagc ttcttcagct tccgggcgag ttcgtcgggg
     5161 tcctcgagcg gctcggtctc gaacggcgtg tgcggcttcg ggatgaaggc gttgacgctc
     5221 gggacgagcg ttccgaggcg accgcgcttc cgcccttcct cgagcatcac gagccggaga
     5281 tcgtcgacca tccggacgag gtcgtccacg tcctcctcgg tctccccggg gaggccgacc
     5341 tgcaggtaga gcttcaggtt cgcgaagccg gcccgcgcga cgaggcgcac cttctcgcgg
     5401 agcatctcgt cggtgacctt cttgttgatc gcgcgccgga gccgctcgtt cccggcctcg
     5461 ggggcgatcg cgagcgactt ctctccctgc cgcgcgagga cgtcgaggag ctcctcggag
     5521 atcgcgtcga tcttgatcga ggagagcgcg atgtggaagc ccatccccga gagcccgcgg
     5581 aggatcggga ggatctcggg gtggtccgcg acggcggtcg cgatcagccc gacgcgggag
     5641 gtgagcgggc ggcatcgctc ggccacggcg aggatcgagg aggcgggaac gcgcacctgc
     5701 ggcgccattg cgtacgcggc ccagcagaag cggcacatct cggagcagcc tcgggccacc
     5761 tcgatcagga gcttgtcgga gagctcggtg tggggcgtca gaaccgtcgt gtggggcggc
     5821 tcgcgaccgg gcccgtcggc cgcgcggagc tggtcgacga cgaccttccc gtcgtcggcc
     5881 gcctcggccc gctcgccgtg caccgcgggg acgtagaggc ccttcagccc ggcgagccgc
     5941 tcgagcgcgg cgcgtcgcgg caggtcggcc cggagcgtcg ccgcgatccc ggggacgagg
     6001 cgttcgccgt ctcccatcgc gaaggcgtcg acgtacggcg tcagcgggag cgggttgatc
     6061 cgggcgcagt cgccgccgac gagaacgatc gggtcgtcgc ctccgcggtc ctcccggagg
     6121 agcgggatgc gtgcggcggg gagcatccgg aggaagtgga cgtagtccat ctcccacgag
     6181 acggagaagg cgacgagcgg aaaggaggcg agcggctgct ccccctcgag cgtccgcggg
     6241 ccgcgtccgc cgagctcggt ctcgtcgtag aagaaacgct cgcaggaaac gtcctcctcc
     6301 cggttgagga ggcgatagac ccactggaag cccaggttcg acatcccgac ctcgtaggag
     6361 ttcgggaagc cgagcgcgac ccggtggcgc ccgccgcgcg cgaaggtctc cgtcgccctc
     6421 tccgaggaga ggaggcggcg tcggcggtcg gcctggggaa cggtgcgctg cttcacgtcg
     6481 tcggcatcct gcgaggacat ctcatgctaa cccggcgctc cgacggcctc gcgagaatgg
     6541 tcccgggccc gcgtctcgct cccttccggt acgattcggc acccgcgcgg aaaccttcgg
     6601 ggcgcattcg acgtttcggg gagtgagagg gacgccatgt actacgtcat cggcgcagac
     6661 ggacagcagt acggaccgat cgacgaggcc accgtccgcg gctggctcgg agaggggcgt
     6721 gtcaacgcga cgagcctctc gttcaagacg ggtgaagccc agtggatccc gctgcgcgat
     6781 cgccccgagc tgggagacgt ccttgcggct ccggtcgccg cggcgccgcc ctacgcgccc
     6841 cccgccggcc cgatgcccgt gacgcccgac gcgccgaagg actggctcgt cacccttctc
     6901 ctctcgatct tcctcggctc cttcggcgtc gaccgcttct acctcggcca cgtcgggctc
     6961 ggcatcggca agctcctgac gttcggaggc tgcggcatct ggtggctcat cgacgtcatc
     7021 ctgatcgccc tcggcaacgt caccgacgcg aagggccgcc cgctcgtcaa gagctgaggg
     7081 gagacgcgag gcgcgcggcg acgaactccg ccgcgcgctc ctcggcccag tagcggaccc
     7141 ggaacgggct cgcccccgag atgaagccgg tgtgccctcc ctggcgcgtc cggacgagcc
     7201 ggacggcacc cgaggcgcgc gcggcgacgc ggtcgagcgc ctcgcggggg aggaacgggt
     7261 cgtcctcggc cgagagacag agcgtgggcg tggcgatgcg tccgacccag ccgatcgcgc
     7321 tcgactcgtc gtagtagtgc ttcgccccgc ggaagccgtg gagagggccg gtgaccgcgt
     7381 cgtcgaactc gaagaaggtg cgcgctctcc ggagccgccc gaggtcgatc cgggaggcga
     7441 ggtcgggatg ccggcgaagg agctgccgcg ccttcgtccg caggccccgg aggaacgtcg
     7501 cggtgtagac gggaccgagc ccgccctcga gctcccgcgc gcccgcctcg aggtcgaacg
     7561 ggacggagat cgtcgacgcg gcggcgacga gccgctctcc cgggtgctcg ccgagccact
     7621 tgaggaggac gttcccgccg agcgagacgc cgatcgcccc cagggcgatg cccggctcgg
     7681 cggcggcgag cgtccggagg aggtggtcga ggtccgccgt ctcccccgag tggtagaggc
     7741 gcctcccgcg gttcggcagg ttccggcgga gcgaggggag gtcgcgcgcg caggagcgga
     7801 agttcagcgc gaccgcccgg agagagcgcc gcgcgaggag ggcgaggagc ccctgcacgt
     7861 agacggagtg ggcgcttccc tccagcccgt ggaggaggac gacgagcggc gcgcgggacg
     7921 gcccgtcgag ccggtcgagg acgagctcgt cgccgtccgg tgtcgcgagc ttctcgcggc
     7981 gcagcggtac gaggagacgc ggacgcgtga ggcggcccca gaacgtctgg acatgcctct
     8041 gcccgagcgc gggggcgggg cggaacggcg ggagcggctc ttcgtgggtc atcggcggcg
     8101 cggcctgggg gcgcgagtgt aggcccggga cgcccgggcg gctcccggct aacatcccgc
     8161 ctccatgccg cgcccgcccc gcctcgcccc gatcccgctt tcgatgaagg gctccgtcta
     8221 ctccgccgtc ctcgaccgcc tcgcggcctg ccccggggag acctatccgc tgcacgtcgg
     8281 cgacacgtac ctcgagcccg ccacgagctg tcggatggag gacctcgccg tcgcggagca
     8341 cccggggatg caccggtacg ctccgccgca ggggatcccg gccctcgtcg acgcggtcgt
     8401 cgagcggctc cgcgcgcgaa cgggcgtccc gctcgagcgg gcgaacgtct tcgtcgccgg
     8461 aggcggcacc tcgggcctcg cctgcgtcgt cggggcgatc gtcgctccgg aggacgaggt
     8521 ggcgatcctc gccccccatt ggcccctgat cgaggggcac gtaaggatgg cgggcggggt
     8581 ccccgtcgac gtccccttct tcggggccgt cgattcgcgg gagtcggcgg tcgccgccgt
     8641 cgagacggcg ctgacgccac ggaccgtggc gctctacgtg aacacgccga gcaacccgac
     8701 cgggcacgtc gtcccccgcg cgtggctcga ggggctcgtc gacctcgccc gccggcacga
     8761 cctctggctc ctcttcgacg aggtctacga ggactacgtc tacgagggag agcacgtgcc
     8821 gggcctctcg cttgcgccgg agcgttcctt cgccgtccac tcctgctcga aggcgtacgg
     8881 catggcgggg aaccgggtcg gctgggtcgc cggcccggcc gcgcccgtcg ccgaggcgct
     8941 gaagctctcg acgcacacgg tctacgccgc gccgaccgcc gcccagctcg cggcccttcg
     9001 ggtcctccgg ggcccgggcg acgcatgggt cgaaaagacc cgtgcggctt acgcctcggc
     9061 cggcgccgcg gccgcggagc ggctcggcgt cccgcggccg cacggctcga cgttcctctt
     9121 cctcgacgtc gccccacgcc tcgacgggcg cgggctgacg gggttcctgg aggacgcggc
     9181 cgaccgcggc ctcgccgtcg cccccggacc cagcttcggg ccctacccga cgcacgtccg
     9241 ggtctgctac acgtccgcgc cccccgacgt cgtccggcgc ggcatcgagg tcctcgccgg
     9301 gctcgtgggc cgctgacgtc ctccccggtg ctatcgttcc cgctccgaac ggaaggagcg
     9361 cgcacgatgg cgtccgaaag ggtccgcggg gccggagccg tcccggcgga ccggggaacg
     9421 gccctcctcg tggtcgacga caacgaggtg aatcgggacc tcttgtcgcg ccgcctcgtg
     9481 gccgccggtt tcgccgtcgc gggagccgcc tcggggcccg aggccctcac ggcgctcgac
     9541 gaggggcgct tcgacctcgt cctcctggac gtgatgatgc cggagatgga cggcctcgag
     9601 gtgctgcgcc gaatccgcgc gcgcttcggc gtcggcgagc tccccgtcgt catggcgagc
     9661 gccctcggcg acagccggag cctcgtcgag gcgctccgcc tgggggcgaa cgactacgtc
     9721 accaagcctc tcgacatgca ggtcgtcctc gcgcgcctcg gcacgcagct cgcgctccgg
     9781 agggcgacgg aggatgtgac gcgcctggcg gcgcagctgc gtgccgcgca ggaccagata
     9841 tccaccctcc tctccaccgg gggcgaggcc ttcgccgact tcggccgctg ggcccgctcg
     9901 atgtcgtccg gcgtggcggg gacgctcggg gcgcgggaga tctcggtctt ccttctccag
     9961 gaggaggcct tcgtctccgt ggaagggacg accgccgaag cgccctcgcc ctccgcggtc
    10021 gcgctcctgc cggcgggcga cggcctctcc gtcgaagggg agagggccgt ggccccggtc
    10081 cgggggccgc gcggggacct cctcggcgcc gtcgtcgcgg acgggaaggg agagtgggac
    10141 gacgtcgagc gcggcctcct cctcggcttc gcccatcagc tcggcggggc gctcgagctt
    10201 cagcggatga aggagcagct cgcggcggcg cggcagcgct cctcccggct cggcgggacg
    10261 acgcagagcc tcgagatcct ctacgtctgc ccgacctgct cccgctgctg gggtccggag
    10321 gtgcgcgcgt gcggggccga cggcaccccg ctgaccgagc cgcgcggctt tccgtaccgc
    10381 gtcctcgacc gctacgcgct ccggttcctc ctgggcgaag gagggatggg cgccgtgttc
    10441 gaggcgtacg acgagaggct ggaccgcgtc gtcgccctga aggtcgtcaa gcccgagcac
    10501 ttccggaacg ccacggtgag gcgccggttc gttcaggagg cgaacgccat cgccgccatc
    10561 cgccatccga acgtcgtcga gctccacgac tccggggacc tcggggacgg ctccatgttc
    10621 ctcgtcatgg agcgcctcca cggccgggac ctcgcggacc tccaccggtt ctcgggcgcg
    10681 gggacgccgg cgcaggtggc ctctctcctc cgccaggggg cggcggcgct cggcgcggcc
    10741 cacggggcgg ggatcgtcca ccgcgacgtc aagcccgaga acgtcttcct ctccccggcc
    10801 cgcgacggat tccgcgtgag ggtcctcgac ttcggcctcg ccaagagggt cgacggggac
    10861 cgtcacctga cgcagagcgg cctcatcgtc gggacccccg cctggatgcc gcccgagcag
    10921 atctccggac gggaggtgga cgagagggcc gacgtctatt ccttcgccgc cgtctgctac
    10981 ctcgccctga cgggtcgggg cgtcacggtg gagcgcgacc tcgacaaggt cttcgtcgac
    11041 gtcgtgagga acccgcctcc gcccgtctcc gcgctcgtcc cggcggcgcc agcggcggtc
    11101 gacgcggcgt ttcgcgccgc gctcgctaag aagcccgagg cgcgcccgcg ggacgtcgtc
    11161 gcctgggccg ccgcgctcgc cgcggcgctc gagggcctcc cctcgaccgg ccccggctgg
    11221 gtcttcccgg cgggcggcgg cccccccggc tcgccctcgg cgcccaccct cgtcacgcgc
    11281 ccccccgcct gattctgctc gcctcgccgg ggggcgcgct ctccgggggg cgctgttcgc
    11341 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccccgg accccccggg
    11461 ttcgaggtag agcacactgt tcccggccgg gctgctagcc tctccggggg gcgctgttcg
    11521 ccgccccccg gaccccccgg gttcgaggta gagcacactg ttcccggccg ggctgctagg
    11581 atcgtcccac catgcacgtc gaggtccgaa aaaagggtga cgtcgtcgtc gccgacctga
    11641 ccggacgact cgtggggggg gtcggggacg agatccttcg cgaggtcgtg aacgagctcc
    11701 tcgccgaggg gtggaagaag atcctcctga acctctccgg ggtccaggtc gtcgacagcg
    11761 ccgggatcgg cgagctcgtc gcgagccaca agctctgcaa caagctcggc gcccagctcc
    11821 ggctcctccg gcttccgaag cgggtcaagg agaccctcca cctcgcggcc ctccttcccg
    11881 tcttccacgt cttcgaggac gagaaggacg cccttcacga cctggccgcc ccggccgagc
    11941 cctccggccg aggctgaacg ggcaagcgcc ccgcccctca gcgcggcgtc ggaatccgcc
    12001 gggcggggat gacgaccggc ggcaccccgg catccgcgag ccgggcgttc catgccgcga
    12061 ccccgtcgat cagcgtccgg gtcgcgtcgc ggagagcccc gtccaccgtc ttcttcagct
    12121 cggcccagta ggcgagggcg cccgccgtcg gcggagcgtc ggcgccggag gcggcgctga
    12181 tcaggccgac gacctggtgg tcgagcgccg ggacgaagtt gaggacgtcc tgcgggctct
    12241 tcagcttcgg gttgacgagc ttctcctcga cggcgaggag ccgcgcgtcg acctccttcg
    12301 ccgcgtccag caccgaggaa cggacccggg atctcttcgc gcgctccgcc agggtggaga
    12361 tctgctcccg ggcgtcgcgc aggttgcgga cggtgccgtg gacgtgcgag agggtgtcgt
    12421 agagctcgcc gaggaaggcg gcctgcttcg cgaggtcctc cttcgtctgc ttcacggcgg
    12481 ggttggggag gacctcgagg gggaccgtcc gggtctcgcc gaacgcggtg aggcggacgg
    12541 tgtaggtccc gggcaggatg cgcggccccg tgctcggtcc ccagaggacc gccttcggga
    12601 cgagcgtcgg tccgagggcg cggaggtccc aggcgaagcg gttgaggccg gcggagagct
    12661 cgagacggtt ctcgagcggc tcctcggcct ccgcggcgag cttctcgtcc cgcttctcgt
    12721 tcgagaagta ccggaggagc ttccctccat cgaggacggc gagcgtgacg acgtcggact
    12781 ccctcgggga ctccttcagc cagaacgaga cgagcgcccc ggctggggcg ttctgcccgg
    12841 ccccctgagg ggccttcgcg aagtcgccgc cgccgcgcag ccggaaagcg gccggcgggg
    12901 tgaagagcca ggtcttcgca gcggcgacct cttccgtcca ctgccggagc ggcgagaggt
    12961 cgtccaggat ccagaaggcc cgcccctgcg tcgcgacgac gagatcgccg tccttcacgg
    13021 cgaggtcggt gatcgggacg gccggcaggt tccgctggaa gcgctgccac cccctcccgt
    13081 cgtcgagcga catccagagc cccgtctccg tcccggcgaa gaggaggccc ttgcggaccg
    13141 ggtcctccct cacgacgcgg gtgaaggcgt cccgcgcgat cccggcgtcg atgcgcgtcc
    13201 agctcgcccc gtagtccgcc gtcttccaga ggtgcggcgt caggtcgccc agcttgtaga
    13261 gcgtcgccgc gaggtacgcc gtcgccgcgt cgtgaggcga ggcctcgatc gagttgacct
    13321 ggatccactc cgggaggcct ttcggcgtca cgttcctcca gctcttcccg ccgtcgcgcg
    13381 tgacgtggac gaggccgtcg tcgctcccgg cccagaggac tcccttcgcg agaggcgact
    13441 cggcgagcgc gaagatcgtc ccgtagacct cgacgccggt gttgtccttc gtgatcggcc
    13501 cgcccgagga gccctgcttc gccttgtcgt tccgcgtcag gtcgggggag acctccttcc
    13561 agctctgccc ctcgtccctc gattcgagga ggacctgggc cgcgtgccag agaacgctcg
    13621 ggtcgtgccg cgagacgagg atcggcgcgt tccactgtat ccggtacttc aggtcgctcg
    13681 cggggcgccc gatcgcgagc tgcggccacg cggtcacctc ccgctcctcg cccgtccggt
    13741 ggtcgtaccg cgtgatcgac ccgccgtagc agccggcgaa gacgacgtcg ggctcgcccg
    13801 gcttcggggc gatccagccg ctctcgcagc cgccgacggg gtgccactcc gcgcggccga
    13861 tctcgcggcc gcgcgagcgg ctcgcgatcg cgacggtcgt gttgtcctgc tgcgcgccgt
    13921 agacgtggta cgggaagcgg tcgtcgacgg cgacccggta gaactgcccg gtcggctggt
    13981 tgtcgatcga cgaccagctc ttcccggcgt cgtaggtgac gtatgcgccg ccgtcgtcgc
    14041 cgacgaggac gtgggaggag tcgtcggggt cgatccagag atcgtggtgg tcgccgtgcg
    14101 gcgtcgggag ggcggtgaag ctcttcccgc cgtcgagcga ccgccagagc tgcacgttcg
    14161 tcacccagac ggtgtcggcg ttcttcgggt ccgcccagac gccggtgtag taccaggccc
    14221 gctggcggag gtcgttctcc tcgttcacct tcgtgaagct ctcgccccag tcctcggagc
    14281 ggaagagccc ccccttctcc gcctcgacca tcgcgaagac gcggcccggc ttcgcgcccg
    14341 aggcggcgac gccgaccttc ccccagatcc cctcggggag ccccttcgtc agcctcgtcc
    14401 acgtgtcgcc gccgtcctcg gagcggtaga ggccgcttcc cgggccgcct gagacgagct
    14461 cccacggccg ccgcacgacc tgccacattc cggcgtaaag gattcgcggg ttcgtcgggt
    14521 cgagggcgag gtcgctcgcg ccggtcgccg tgtcgacgaa gaggacgcgc gcccacgtct
    14581 tcccgccgtc ccgcgtccgg tagacgcctc gctcctcgct cggcccccag agcttcccct
    14641 gcgccgcgac ccaggccgtg tcggggtcct tcgggtggac gcggagcgcg gcgatctggc
    14701 gggtcgcctc caggccgagg tgcttccacg tccggcccgc gtcggtcgag cgccagacgc
    14761 cgtcgccgtg cgagacgttt ccacggatcg gcgcctcgcc catcccgacc cagacgacgt
    14821 tcgggtccga ctcggcgacg gcgaccgcgc cgacggagcc ggtccggaag aactcgtccg
    14881 acaccggctc ccagctcgtg cccccgtccg tcgtcctcca gaccccgccc ccggtcgccc
    14941 cctggaagtg gacgtttctc tgcccgcgga tgccggagac cgccgtcacg cgcccgccgc
    15001 ggaacggccc gatgttccgg aaccgcattc cgtcgaagcg gttctcgacg gggggctttc
    15061 cgaccgcggc cttcgagtcg accggcgcgg cgagggccgg agcggtgacc ggggcgagaa
    15121 ggaagaagac gaaggccggg gcgaagcgcg ctgcgtctct cacgggagcc tccggacggg
    15181 gacgataccg gagcgccggc cacgcgggcc cgtgcgaaat ccggctgcgc ggggtccggc
    15241 gtaggagggg cgtgcggggc gaggacgctc ccgcgccacg gaggcgcgac atgaacggac
    15301 tcgcgacgag gcccaggacc ggagaagagc tcgaccgcag gatccggatg gagtgggcgg
    15361 tccggcaggt gcagaggggc ttcaaggacg ttcaggaggc cgccctcgcg gcccgcgtgc
    15421 ccgtgaccca cctcgcgtcc gtcgtcggag ccgacgcctg aggcgtcgcc gccgcgccgc
    15481 gcgcgggaga gaacgttccg gagacgaaac gggggcggcg acccgagcgg gtcgccgccc
    15541 cctggagctt ccggaggggc tttcctaggg ctggagctcg gaggtcacga cggcgccgcc
    15601 catctgcggc agcgtcgtgg aggtcttgac gaccttccgg ctcgcggagt cgacccagag
    15661 cgtcgtcgac cccggctcac cctcggccga ggtgagctcg accttccacg ccgagaacgt
    15721 cccggcggcc accttcacct cctcggtgcc ggcgaccttc agctgccgga gcgtggcctt
    15781 ctgcttctgg acgtcgaaat tgcggtaggt cgccgcgtac ccggccgcga gcggaaggca
    15841 cgcgatcgag tcgtacgtgg cgttcccgtc ggcgaagagc ggcccgccga ggtcggccga
    15901 gatcggcttc ggctcggccc ccatcgccat cgtcccggtc gccttgccgt cggcgaacgc
    15961 gaggtcgatc gtgaccggac cctgacgaat gctccgcttg accggggcca gcgttccctt
    16021 cgcgagcgtc gtgacgtcgg tcgcctcgcc catcggcatc tttgccgtgt cggagacgac
    16081 ccaggtctcc ccgtccgcct tgatgacgcg tgtggccgag acctgcatgc tctgcccgcc
    16141 ggccgagatc gtcccggcgt aggcgtaggt gccggggacg ggctccgcga ccggcttcgg
    16201 cgcgccgacc gcggccgcgt cgaccttctt cgcgagcgtg accgtcttcg ggtcgaccgt
    16261 gatctccttc agccgggcga cgacctcggg cgtgccgccc tcctggaacc ggccgccgag
    16321 gtgcttcgcg aggaaggcct cggacgcggc catcgccgcc atgttgttga cgggccgcgc
    16381 gaagccgtgc ccctcgtcgg gggcgcagat gtactccacc gggaagccgc ggtcgcggag
    16441 cgcgacgacg atctggtccg actcggcctt cttcacgcgc gggtcgttcg ccccctggat
    16501 gacgaggagc ggcttgacga tcttcgccgc ggagttgagc ggcgaggcgg cctcgaggag
    16561 cttctttccc tcgggtgtgt tcggattccc catccgctcg tggaagatga tccggatcgc
    16621 ctcccagtac ggcgggatcg tctcgaggag cgtcaggagg ttcgacgggc cgacgagcga
    16681 gacgcccgcg gcgtagaggt cgggggtgaa ggtgaggccg gcgagggtcg cgtagccgcc
    16741 gtaggagccg cccatgatgg cgactttctt cgggtcggcg atcccctcct tcacgaggtg
    16801 cttgacgccc cacgtgatgt cgtcctgcat cttcaggccc cactccttgt tgccggcgtt
    16861 caggaacttc ttcccgtagc cggtggagcc gcggaagttc ggggcgagga cggcgtagcc
    16921 gcggttcgcg aaccactggg cgtagttgtt gaagccccat ccgtcgcgcg cccacgggcc
    16981 gccgtgcggg aagacgacga gcggaaggcc cttcgcgggg actcccttcg ggacggtgag
    17041 gtaggccggg atcacgagcc cgtcggagga cgggtaggag atcggtttca tctccgccat
    17101 gtgctcgcgc gggaggcgct cgcgggagac gtactcgacg gagagcttct tcgtcttccg
    17161 gtcgaagagg tagcgcgtgc cggggtcgac gtcggagtag gccgtgacca tccagagctg
    17221 gtcgtcggcc gtggagccgg cggggatcac ctcgcggccg gggagcttct tctcgaggag
    17281 acggtagtcg gcctcccacg ccttgtcgcg gaagtagatc cgcgtcttct cgtcctcgta
    17341 ggccgtggcg acgagctcgt cggtcgcctc ggagaagacg gcgttcccga agtcgacgcg
    17401 ccccttcggg tcggtctcga cgggctcttc cttcccggtg gccgggtcga agaggatgag
    17461 gcgggtcagg tccggctcac ccttgttcga ttccatgtag acccgcttgc cgtccttgtg
    17521 gaagcggacg gggccgcagc tctcgaagac ggagcaggag tagaccttga ccagtccttc
    17581 ggtgtcgacc cggaggatct cggtgtcgcc gttgtcggcg atgcgcgcgg cgagccggag
    17641 ctggccgtcg aggtcgaaga cccagcccga gatcttctcg gtgttcttct tcacgagcgt
    17701 ccgctcgccg gtcgagatct tcaccttgta gaggtcgtgc caggccgcgt cgcggtcgtt
    17761 caggccgacg aagacggtgt cggggtccat cttcgggacg gcgtagatct gagcccggac
    17821 ccccttggcg tcggacaggt tccgcgcggg gggggcatcc ttgccggctt cggccttcgc
    17881 ggccgggtcg acggcccaga cgttgtagtt ctcgtcgccg tcattgtcct tgacgaagag
    17941 gatgtacttc gagtcccggc tccagaagaa gccggccacc gggcgtttcg gctccgcggt
    18001 gacgaggtgc gccttgtcgt acgggtcgcc cgtcttcttc acccagacgt tccgggtctc
    18061 cttccagggc ttcagaaagg cggtccactt cccgtcgggg gagagctggg cgccggcgat
    18121 ctcggggttg ccgaagaaga gctcccggtc gaggagcggc gggagcccct tcggggcggg
    18181 agcggcggcg gacgcggcca tcgggacggc gagagccgac gcgagcagga gccgcgcgag
    18241 gcgcggccgg aagggcgaac aggtcatcgt cgaggcctcc ttgagccggt tgagagcgtg
    18301 cagttgcact cgataacgaa gtacgtcacg gggcgtccga agttccattc ggagacggtg
    18361 tctccgcgtt cgtgccggta atgagccggg ccgagcgggc ccaggtcagg tagctccagg
    18421 cccactggac gaggacgagg acccggttgc cgaactggac gatgtagagg aggtggacga
    18481 agagccaggc gagccacgcc ggatacccgc cgaagcgaag gccgccgatc tcggcgacgg
    18541 ccgaggcgcg gccgatcgtc gcgaggctcc ccttgtccac gtagcggaac gggcggccgg
    18601 gccgcccttc gagccgcgcc cggatcgtgc gcgccacgtg ccgtccctgc tgcatcgcga
    18661 ccggcgcgac gcccgggagc ggacggccgg aagtcggctt gacgagcgcg aggtcgccga
    18721 tgacgaagag ctccgggcgc cgcgggaccg tcaggtcggg ctccacgacg acgcgccctg
    18781 cccggtccac ctcggctccg gccgcccggg cgacgaggcg gccgagcgga gagccttcga
    18841 cgcccgccgc ccagaggacc gtccgggcgg ggacctcctc cacgacgtcg ccgcggcgga
    18901 gcgtgacgcc gcgagggccg actccggtca cgaggctccc ggtgcggagc tcgatgccga
    18961 gccgggcgag gtctcgcgcg gcccgcgcgg aaagcgcggg agcgaacgac ccgaggaccc
    19021 gctccgtccc ctccacgagg aggacccggg cctcggcggg gtcgatcctc cggaactcgt
    19081 cccggagcgt gtgccgggcg atctcggcga gcgctcccgc gagctcgacg cccgtcgggc
    19141 cggctccgac gacgacgaac gtgagcgcct ccctccgccg ggccggttcc tcctcccgct
    19201 ccgcctcctc gaacgcggcg aggacgcgcg cccggatgcg cgtcgcgtcc tccagcgtct
    19261 tcaggccggg ggcgagctct tcccactcgg gacggccgaa gtaggagtgc cgcgcgccgg
    19321 tggccacgac gaggacgtcg tacgagagct ccccgtccgc gagcaggagc cggcggcccg
    19381 cgacgtcgac gcgcgtcgcc tcgccgagga ggacgcggac gttcttctgg cgtttgaaga
    19441 gcgcgcggag gggggtggcg atgttcgccg gcgagagccc gccggtcgcc acctgataca
    19501 ggagcggctg gaagaggtga aagttgcggc ggtccaccag agtcacctcg accggggcgc
    19561 cgcggaggcc gcggacggcc gagaggccgc cgaagccgcc gccgacgacg acgacacgcg
    19621 gagcgcgcgc actggccgtc atcgtccctc cggacgatcc gggaggagct cgtcgtcctc
    19681 caccccgagg agacgggcga gcgcgcggcg gcagagctcc gtcggcgcgg cgtcgagggc
    19741 gtctcgggcc gcgtcgccgg actctgttcc cgcgggcctc gcgaggccga cggccacagg
    19801 ctccccgggg cggagccgcg cgcggtcgca gccgcgttcc tcgagcgcgg agaggagggc
    19861 gcggaacggg tcgagcccgt tcccgaggag cgcggcaacg agcggctccg ggacgcagcc
    19921 cctctcgagc gccgcgtcca ggtggtcgag ggccagcgcc tcggcgcgcg ccgggtcgac
    19981 ctcgaagagc gcctcgagcg gcgagacgcc gaacggaagc accgcctccg cgacccgcgc
    20041 gagctccgaa cgccccgcct cgccgagcgg cccgaagagt gcggcgagct ccgaggacga
    20101 cacgcaggcg ggacagcgtt cgaggagggc gttcgcgagc gggccggcgg agccggcgtc
    20161 acccgccttt gcgaggagcg aggcggcttc gaagctcgcg gcggggcgcg tgcgcgcggc
    20221 ccggagcgcc gcccggagcg gctcgctctg ccggacccgg tcggcgtcga gggcccggca
    20281 gaaggcgccc gcctccccgt cgtcgccgtc gagcgggacg tttccgcgtg cccacgcctc
    20341 ggcggcgacg cggtcggccg cggccgggtc gaggcgggcg agcgcgtcga tccatgtccc
    20401 gcgctcggcg ggcgggaccc ggcccgaggc gagctcgcgc tccaggtggg cggcgaggcg
    20461 cggggcgggc ggccgcgcct tctcgagcgc gagccgccgg taggcgaagg cgagcccttt
    20521 ctcgtcgagg agctcggtga cttcctcctc ggacgccgtg ccgcggagcg cccggctgag
    20581 cttcagctcc gcgcggagct cggggggggc cgccgaggac cgcgcgagct cggccagcag
    20641 aacgacgcgc tcgcgcgaga tgacggccgg atcgtcgtcg ccgccgaggt cgagggccgc
    20701 gaagcggacg atggcgaaaa ggttgcagta gcggtcccgg ttcccgtcgc cctccgcctg
    20761 gaggaccggc cacgcgctga cggagcaggc cgatgggtcg gccgaggcgg cgcgagccac
    20821 cgcgagcagc tcggcgaaga ggccgaccca gtcccggggc gagagcgagc ggtcggagag
    20881 gagccccagc gtggccagcg cgccgaggtg ggggcggcgg aggacctcgg aacggaagag
    20941 gccggcgtcg agagggagtc cggtcagcgg cccgagcgcg agcgctccgg cgcggaggga
    21001 gggctcgggc gagcggaatg cctcgaggag cgtcgcggcg agggcgcccc ggatgctcga
    21061 gcccttcgcc tcgtcgctcc ggccgaagtg cgcctcggcg ggcgggggcg ccgcctcgat
    21121 cgcggcctcc gcgacgcggg tgagcgcgag caacccggcg accgacgggg gcccggcggc
    21181 gcagtcgagg agcgtccggg cgaagaggcg cgccggcgcg tggaagcgga gctcctcgaa
    21241 cgaggacgag aaggcgtcgt cgaggcttcc ggcgaggagc gcctcggcga gctccggccg
    21301 ctcggcggcg atccggtcga tcctcgcccg gtccacggcc gtcacgagct ccgcgagctc
    21361 ggtcgtccag gcctcgtcgg gaagctcgag cgaatctccg gcggctcccg aggccaccgc
    21421 ctcggccacg gcggggagcg cccaggcggg cgcctcgccg ggcccgtcga ggaggacgaa
    21481 cgcgaccgct cgacggagcc agacggggcc tccggcctcg acgagcgccc gcaggtcgcg
    21541 gcggaggccg gagcgctcct ccgtttccgc aagatcgacg aggagcgcga ccgcgcgctc
    21601 ggcgagcggg gcggaggcgt ccgcgtcgga gacccgggag agccgcgcga gcgctccgcc
    21661 cgggtcgcgg tgtgcgaggg cgacgtggct ccctgcgggc gcccccggca ggagcgcgag
    21721 ctcggcccag tcggccccgg aggcccgcgc cccgagccgc tcgagccgcc gaagctgctc
    21781 gacgacgcgc gtcgccgcct ccgtgtcgga accttcctcg agcgggagga ccgcccggga
    21841 gatctcggcg aagcggtcgt cgaaccgcac cgcctccatt ccgtcgagac ccaggaggcc
    21901 gccgtcggga agcgggaaga gctcggccca ggcgccgagc ggcgccgagc ggagaaggcg
    21961 gccttgggcg tcgaagagct ccgcgacggc gagaggctcg ccgccccacg aggagtccgc
    22021 ggagggggcc gtgcggatcg acccgcgcgc gaggccctcg gcgtggcgat agaggaaccg
    22081 ggaccgcccc tcgccgcacg cctcgagggg gtcgggaacg acgaggccgg tggcggcggt
    22141 ccagaggacc tcggcgcgcg cgccgggaaa gagaacggcg gcggacgagc cgccgaagac
    22201 gcgcggggag gagacccggc cggcgagccg cctcacgtcg gcctccggta tcgagccggc
    22261 cggggcctcc ccgtcgggac cgacccggaa gaggcggaag ctccccgtct cccgacggac
    22321 gagccagacc tcgtcgccga ggaacgcggg ctccgacccg cgcagcgcgg tcgtccgggc
    22381 ggtcgcgcgg accgagccgt cggggccgcg gaagacgaac cgcgcctcga cgtgcgcctc
    22441 gcttgccgtc cgcgacggga ggcgccgtaa accgtcgggc gtcggggccg tcacccgcgc
    22501 gccgccgtcg gtcgcgccga ggacgaatcc ctcgtcgccc gccatgggga acgccccgat
    22561 caccgatccg ccggtctcgc gcaggacgag ccgcctcgcg gccggctcga cgacccagac
    22621 gaggccgccg ggcgtcacgc cgaacccggg ctcgacgcgc cctcgcggcg gagcggccgc
    22681 gagcggcagg gcgccgagga ggagcgcggc ccccgccgcg cggcggagcc gccggccgga
    22741 gcgccgggtc gggagggcgg gccgcatcgg tcgcgcgagc atatccgcgc ggcgccttcg
    22801 accgtctgcc gcgtcgcggc accacaacgt aaactgcgcg cgtcttcccg acacgatcgg
    22861 acgaggaggc ccccgtgccc gagagcaacg tcctgtccgt cctcgcgagc gaagggatcg
    22921 aggtcctcgg accgatcggc ccgctgtacg acgagatcct cacgcccgag gcgctccgct
    22981 tcgtcgcctc cctccagcgc gccttcggcg cgcgccgcga ggagctcctc gagaggcgcg
    23041 tcgaggtgca gaggcggatc gacgcgggcg tcctgcccga cttcctcccc gagacggagg
    23101 agatccggaa gggcggctgg gcggtcgcgc cgatccctcc gatgctcctc gaccgccgcg
    23161 tcgagatcac cggccccgtg gaccggaaga tggtgatcaa cgccctcaac tcgggcgcga
    23221 gcgtcttcct cgccgacttc gaggacgcga actcgcccac ctgggcgaac tgcgtcgagg
    23281 ggcagctcaa cctgcgcgac gccgtcgacg ggacgatcgg gtaccgcgcg cccgactccg
    23341 gcaaggagta caggctggcg gagaagaccg ccgtcctcat ggtgaggcca cgcggctggc
    23401 acctcctcga gaagcacctc ctcgtcgacg ggaggccctg ctcggggagc ctcttcgact
    23461 tcggcctctt tctcttccac ggtggccgcc gcctgatcgc gcaggggaag acccccgcgt
    23521 tctacctccc gaagctcgag caccacctcg aggcgcgtct ctggaacgac gtcttcacga
    23581 aggcgcagaa ggacctcggc gtcccgcacg ggacgatccg cgcgaccgtc ctcatcgaga
    23641 cgatcctcgc cgcgttccag atggacgaga tcctctggga gctccgcgac cactccgccg
    23701 ggctcaactg cggccgctgg gactacatct tctccttcat caagaggctc ggacgcctcc
    23761 ccggctacgt cctccccgac cgcggcctcg tgacgatgga gaagcgcttc ctcgcctcct
    23821 acgtcacgct cctgatccag acctgccatc gccgcggaat ccacgcgatg ggcggcatgg
    23881 cggcgcagat cccgatcaag aacgaccccg ccgcgaacga ggcggcgctc gcgaaggtgc
    23941 gcgccgacaa gctccgcgag gtgaaggccg ggcacgacgg gacgtgggtc gcgcaccccg
    24001 gcctcgtccc gatcgcgatg gaggtcttcg acgcggggat gccgcagccg aaccagatcg
    24061 cgaccgcgat gcccgccgcc gagatcaccg cgaaggacct cctcgccgtc cccgaggggc
    24121 cgatcacgga gaaggggctc cgccagaacc tgaacgtcgg catcctctac ctcgaggcgt
    24181 ggctccgcgg aaacggttgc gtgccgctct acaacctgat ggaggacgcg gcgacctcgg
    24241 aaatctcgcg gacgcaggtg tggcagtggc tgaagaaccg cgtgacgctc gccgacggga
    24301 ggacgctcac ccccgagctc tacggccagg tcttcgacga ggaggtcggc gccgtccgcg
    24361 cccgcctcgc cgaggacggg gtcggcagcg gccgctatga gctcgccgcg cggctcttcg
    24421 acgagatgat ccggagcgag gagctgccgg agttcctgac gctctcggcg tacgaggagc
    24481 tgatgaagat gggggcgtag ggaagcgaaa ggggaggcct cggtgcctcc ccttcgcgtt
    24541 ccgtagcttc ttcgtcccgt cgcggcgctc gcgccgcgag cgccgcgacc tacagcgtct
    24601 tcgccatgag ggcggccagg tcggccgtcc gcatgctgta gccccactcg ttgtcgtacc
    24661 aggtgacgac cttcacgaag tcgctcttct ccttgccgag gaccatcgtg aacggcaggt
    24721 cgaccgagga ggagtgcggg tcgcccttga agtcgaccga gacgagcggc tcgtcgacgg
    24781 cggcgaggat tcccttcatc gctccggcgg cggcgtcgcg gaacgcctgg cggagctcgt
    24841 cggtcgtcgt cggggtcgcg agctgcgccg tgaagtcgac gacggagacg gtcggcgtcg
    24901 ggacgcggag cgagtagccg tcgaacttcc ccttcaggtc ggggatgacg agcgcgacgg
    24961 ccttcgcggc gccggtcgtc gtcgggatga tcgacagccc ggcggcgcgc gcccggcgca
    25021 ggtcgctgtg cggcaggtcg aggattcgct ggtcgttcgt gtaggcgtgg atcgtcgtca
    25081 tgaagccgcg ctggatcgtc cagcggtcgt ggacgacctt ggcggccggc gcgaggcagt
    25141 tcgtcgtgca ggaggcgttc gagacgacgt ggtgcttcgc ggggtcgtag gacttctcgt
    25201 tgacgccgag gacgatcgtc aggtcctcgc cctcggcggg agcggagatg atgaccttct
    25261 tcgcgccgcc cttcgtgatg tggttctcgg cccccttgac ggccttcccc ttcttgttca
    25321 caccgtcgga cttgatcgtg aagaggccgg tcgactcgat gacgatgtcg acgccgaggt
    25381 ccttccaggg cagggcggcc gggtcggtct ccttgagcat cttcaggtgc ttgccgtcga
    25441 cgaggaggtc ctcgcccgcg acctcgaccg tcccgtcgaa gcgcccgtag gtcgagtcgt
    25501 acttcaggag gtgggccatg gtcttgagat cgccgatgtc gttgacggcg acgacctcga
    25561 gctcggtggg gtacttgtcg cggatcgcct tgaggacctg ccggccgatg cggccgaacc
    25621 cgttgattcc gactttcgtg gccatcgtgg gcgtctcctt ccgggggcat tccccgcggg
    25681 tgatgctccg ttatacgacg gtcggccggc cgcgggagac cggaaaaccc tcagcccggc
    25741 cgttccgtgg acgcgatggg agcggtggcc tcggcggggc cgtccgggcc ggcgccgcgg
    25801 gccgagcagc cgcggtgcca gaacgtcgcg ccgaggagga gggcgacgcc gcccgcgagc
    25861 gccgcgaccg cggccttctt ccgggcccgc ttcgccggct ggatcgagag cgtctgcgtg
    25921 agcgcccgct ggagcttctg gctcggcgtg agcggctcct gcggctcctc ctcgaaccag
    25981 gccgggacgc tgccgaagcg gatcgcgacc ccgttcggaa gcggcgcctc gtcgacccgg
    26041 cgggcgtcga ggaacgtccc gttcccgctg ccgaggtccc gaacggtcca cccttcgccg
    26101 tcggggaaga cgagggcgtg acggcgggag accgtcggcg aggagaggag gacgtcgcag
    26161 gccgggtcgc gccccacggt cacggcgtgg tcgaggtgga acgtgtgccg ggcgccgtct
    26221 tcctcgagcg tcagtgtggc ccgaggggaa ggctcggagg cggaacgcgg ggcgggtcgg
    26281 gccatcggtc agatccggtc ggggagggga attccctcct ccgggaggtc gagggcggag
    26341 gggacgtcct ctcctccggg ggcgaggacg aaagcaacgt cgccgacggc ggtccccgtc
    26401 ggtccggggg cgaaggcgtc cccgagcgcg gcgaagaagg ggccggagtc gttccgggtg
    26461 agcgcgaccg tcgggtcgag gccgagcgcc gcggcgcgcg cgagggtcgc gtggtcggcg
    26521 aaggcgccgg cggcgtcggg cgtgtggtcg acgccgtccg tacccgcgac gaggagcgtg
    26581 gcgccggcga tcccgtcgac gtggaacgcc gtcgccaggg ccagctcgcg gcaacgtccg
    26641 ccctggcccc gccccttcac ggtcacggtc gtctcccctc cgaagagggt gacgagccgc
    26701 ctcggcggcg cgccggcgcg gcggacgagg tttccggcag aggcgccggc gacggcgagc
    26761 cgacgggcgg cggcgcgcgc ctcgccggag aggagctcgg gcaggacgcg gacatcggcg
    26821 ccgagccgga gcgcttcccg tcgcgccccg tcgagcgccg tccggacgtc gccgaggagg
    26881 accccccacc gctggccccc gtcccggacc tcctcgtgcg cgggaggcgt ggcgaggaac
    26941 gagcggaccg acggggggac gagcggcagg attcgccagg tctccaggag cgtcgcggcg
    27001 gtgcggcggg acggcgggac cccgagggtc gggcccgagg cgacgaggtg ccagccggcg
    27061 tcgccgaggt cggagaggac gagcgtgacg acgtcggcct gccgggccgc ggcggcgagc
    27121 cgccccccct tcagacgcga gagcgccccg cgcacgaggt tcacgtcctc gacgggcgcc
    27181 ccggcggcgg cgaggaggga gacggtccgg accttgtcgg cgaggggaac ctcctcgggc
    27241 ggcagggcga gaaggctcga gcccccgccc gagaggagga cgacgaggag gtcgtcccgc
    27301 ccgaggccgg cggcgagcga caggacctcg cggcccgccg tcaggctccg ttcgtccggc
    27361 acggggtgcg acgaggggag atagcgaccg ccggagggga gctcttccgg cgggacgccg
    27421 gccggggcga caacgaccgc ctcgttcacg aggttcccga tcgcccggag agccgccgtc
    27481 gccatcgccg gcgcggcctt cccgaccgcg agcaggacga cgcgccgggg gacgaagacg
    27541 aactcgcgcc gtccggggtc ctcgaccgag cggacgacga cggcgctgcc ccggcgggag
    27601 agggcctccg acacgagccg cccgggctcg acggccgaga ccgccgcgcg gtagagggcc
    27661 ctgaggaagg agcgggggtc caaggagcag aattctagcg aaccggcgag cgccccgcct
    27721 ctgcgagaat cgggccgggc cccgtcgggc catggaggtg acacatgcct caggtcgacg
    27781 tcgaccgaat cgtcggttcc atccgcgagg gcgccggagc cgttcgcgag aagacacagg
    27841 acgccgtgga ggacgtggcc gacaagatcg aggacgctct cgagaaggcc gggaccgaag
    27901 ggcggaagct ccagcgcgag gtgaaggaag agctcgcgag gcggtggagg caggtcgacc
    27961 gcgtcggccg cgagaacgcc ttcctcatgg ccgccggggc gctcgccgtc ggcgtcctcg
    28021 tcggctggct catctcccga gaccgccgca gctgacgccc gcgtggacct cgccgctcgc
    28081 ttctcggagc tgaagtccgg cttcgcccgc tccttctggg tcgccaacgt cctcgagctc
    28141 ttcgagcgct tctcgttcta cggctcgaag atcgtcctgg ccgtcttcct cgccgagacg
    28201 gtcggcctcg gccccttcgg cgtcagcctc gtcgggctct acgggttcgc cgtctacttc
    28261 ctcccgatcc tcgccggtcc cgtcgtcgac cgcttcggct tcaagaagag cctcgcggcc
    28321 tgcttcgcga tcttctcgct gggctatttc ctcatcggcc tctcggggat gccgatgggg
    28381 cagccgctcg tgtcggccgt cgggacgaag acctgggtcg tctgcgccct cctgatcacc
    28441 gccgtcggcg gctcgctcat caagccgtgc atcgtcggca ccgtcgcgcg gacgacgacg
    28501 aaggacacca aggccctcgg ctactccatc tactacaccc tcgtgaacat cgggggctgg
    28561 ctcgggccgg tcctcgcgag ccaggtgcgc gtctccctcg gcatcgcgca ggtcctcgtc
    28621 ggcgcgtcgc tcgtctcgtt cctgctgttc ctcgggacgc tcgtcttctt caaggagccc
    28681 gcgcgcgacc ccggcgccga ggagcggact ctgggcaaag tcctgaagga cgcggcgctc
    28741 gtcctcgcga acggccgatt catcggcttc ctcgtcatct tcaccggctt ctgggtgatg
    28801 ttctggcagg tctacgacct gttccccttc tacgtccgcg acgtcctgaa ggtggagcgt
    28861 ttcgagctca tcggctcgct cgaggcgttt tccgtcatat tcctgaccgt tcccgtcgcg
    28921 gcgctcatga agaaggtccg ggccgtgcct cagatgacgc tcggcctcgc ggtcgggagc
    28981 tgctcctggc tcctcctcgt cgtctcgcag acgtggcagg cggcggccgc cgcgatgttc
    29041 ctcctcgccc tcggcgaggt gctccaggcg ccgcgctact acgagtacat cgcggacctc
    29101 gccccgaagg agcaggtcgg caccttcatg ggcttcgcgt tcctcccgat cgccctcggc
    29161 gccctcctcg cgggccccct cgggggggtc ctcctccaga gctacctcaa ggagacgatg
    29221 cagcccgcga aggcctggat gatcctctcc ggcctcggct tcgtctcgac cgccgccctc
    29281 ctcgcctacg atcgcttcct cgtccgtcgc tgaatccgag ggtcaggtct tgcgatgagg
    29341 cattcgcgag ccgcgaatgc ctcatcgcaa gacctgaccc catccctgac gacttcggca
    29401 agtaccccgc actcaagggg ctgtcgtact tcctctccac gctcctcctc ctgatgggcg
    29461 tgcagtcgat gtaccagaag ttctacgcgg cgcgctcccc gaaggacgcc aagacggccg
    29521 tggcgatctg gatcgtcggc acgatcgtcg tcgagtcgat cgtcatcgcg atcgccgtct
    29581 acgcctcctc gtacaacgcc gagcacaaca tgggctggga cccgcgctcc ctcgtcctga
    29641 acgcggcccg ccacatggtc cccggccccg tcgggatcct cctcctcggc gccgcctgcg
    29701 cggtcgtcct gtcgaccggc atgaactacc tcctctcctc cagctccaac gtgatgcgcg
    29761 acatctacca gcgcttcgtc aacaccgacg ccgacccgac gaagatggtc gcgctccaga
    29821 aggtgttcgt cctcgcgctc ggcgtcctcg cgttcctcct catcttcgtc ccgacctact
    29881 tcaagctgca gatctcggtc ctgcagtacg cctatttcgc ctacacgatc tacggcgtcg
    29941 ccgtgacccc ggcgctcttc gcggcgctca cctggaagcg ggccacccgc cagggcggcc
    30001 tcgcctcgat cgtcgtcggc acgctcgcga ccgtcttctt cgaggtcgtc ctgccgcgga
    30061 tcgcgccggg cgtcatcgag ggggccaagg gggccggcgc cgcctacttc gggggtgacc
    30121 cgtgggggat cccgagcatc taccccgcgg cgatcctctc gatcggcgcc ctcgtcgtcg
    30181 tcagcctcct gaccccgccc ccgaccgaag agcagctcgc ccccctcttc ggcgagaaga
    30241 agatgtaggg gcccggggag cgcggtatcg tggactctcc tcgcgagggg tcgggcgccc
    30301 gcgaggagag agccgcggga acggaaggga gactgccgtg ggcctcggtt tcctgtacga
    30361 gtcgccgttc ttccgggagc cgttcccgtt ctcgctcccg acgttcctcg ccctcgagct
    30421 cctcgtcgtc acgggcctca cggccttccg cctcgccgtc ctcggctatt ccctcgcgga
    30481 ggcggcgtcg ctcttgcccc tcgacgccgc cgtcgcggcg gtcctcctcc tcgcgacgct
    30541 cgggaacccc ctgaaggcgc ctctcgtcta cggcgtcgcc tacatcctct tcgtcgtcgc
    30601 cttcctccgg caggagcctc cgtccggctt ctggaggtgg ttcgggacgt tcgtcgtcct
    30661 cggcctcctc tggggcgtct tcgctctcct ttggcggatc tcgcccctcg tgaccggggc
    30721 gctccttctc tcgtacatcc tcgtccgcgt cctcctcccg ctccactacc gcaacgaggc
    30781 ccgggaggtc ctcgcgaagg ggaagccgtt cccggtcggg cagcggttcg tcgcgacgct
    30841 cgccgaccac ggcagcttct ctctggcgcg ggagaagttc cggtggttcc tcgacgacgg
    30901 tgcccggtcc ggcgagggga cacgggccgt gcgcgctgcc catcggcagg tccgcgcgcg
    30961 aatgaaggag ctcggcgtct accacgtcgc gtgggaccgg ctccacgccc ggacggaacg
    31021 gctgcaggac gggcgcgaac tctacctcta cgagggacgg acggtcggtc tcctccggag
    31081 gaaggaggac ctccccgcgg gcggccggct cgagaacacc gccgccgccg aggagtcgca
    31141 gctcccggcg cttctcctcg acgtggggcc catcgcggtg cgcctcgtcg cctcgccggg
    31201 cggagggtac cgcgtcgacc tgccgccgcc cgagatcccg ctccgtctcg tcccctgaga
    31261 agaccatggg gtcaggtctt gtgatgaggc attcgcgatc gcgaatgcct catcgcaaga
    31321 cctgacccca tcctactttt cgtcggtgag gccccaggcg cggaggcggc ggtggagggt
    31381 cgtccggtcg aggccgaggc gctccgcggc gcgggagacg ttgccccggc actcctccag
    31441 cacccggacg acgacgcgcc gctcgaaagc gtcgcgcgcg tcggcgagcg tctggcccgg
    31501 caggaggccg tcgttctctc cggtggccgt ccgcggcagg cggaggacgt cgccggggag
    31561 gtcttcgggg ccgatgacgg cgccgtccgc gaggaggagc gcccgctcga cgacgttctg
    31621 cagctcgcgg acgttccccg gccagggacg ccgcttcagc tcctcgagcg cctcgggcga
    31681 gaacgtcggc ggccgccggc cgcgccgacg cgccagcttc ccggcgaagt gggcgacgag
    31741 gacggggatg tcctccgtcc gctcggcgag cgtcgggacg cggatcggga cgacggcgag
    31801 gcggaagtag aggtcctcgc ggaacgtccc ggccgcgatc atcttcggca ggtcacggtt
    31861 cgtggcggcg acgatccgca cgtcggtctc gatcgtccgc gtcccgccga cgcgcgagat
    31921 cttcccgtcc tggatcgccc ggaggatctt cgcctgcgtc cgcggcgaca tgtcgccgat
    31981 ctcgtcgagg aagagcgtcc cgccgtcggc ggcctcccac ttccccttcc ggtcctccgt
    32041 tgcgccggtg aacgagccct tcacgtgccc gaagagctcg ctctcgatca ggtcctcggg
    32101 aatcgccgcg ccgttcacct cgacgaacgg gccgcccgcg cggggcgaga ggcggtggag
    32161 agtccgcgcg gcgatctcct tgccgacgcc gctctcaccc gtgatcagca cccgggcgtc
    32221 cgacgccccg gcgcggcgga gctcctcgcg aaggcgcgtc atcgcgggcc cctcgccgag
    32281 gagcgtctcc tcctcctcga gccgctcgcg ctgccgggcc acctcccccg cgaggacgtc
    32341 gcgctcgcgg gcgagccggc tcctctcgag cgccttctcg agcgtcagaa gcaccctctc
    32401 gagggcgagc ggcttctcga ggaagtcgtc ggcgccgcgc ttcaccgcct gcacggccgt
    32461 ctccaccgtg ccgtggccgg agatcatcac gaccggcagg tcgtgcccgc gcgcgcgcag
    32521 ctggtcgagg acggagaggc cgtcgacgtc cggcagccag acgtcgagga agagcgcggc
    32581 cggcctcggc ccggtcctct ccccgaggcg cgagaggacc gacccgccgt cctcgaacgt
    32641 ctcgacgcgg tacccctcgt ccccgaggat ctgcgcgagc gtcttgcgga tcccggcggc
    32701 gtcgtcgcag acgaggaccg gcagcccccg cgggccggga gcgctcacgg cctcctccgc
    32761 gaacgagacg tcaggccgcc ggccactcga agacgaatcg gcatccccgc ggggcattct
    32821 cctccaggat cacgcgcccg cggtgctccg ccgcgatccg cgacgtgatc gagagcccga
    32881 gcccgtagcc gctcgccttc gtcgagaacg tcggctcgaa gacgaggtcc cgctcctcgg
    32941 gcgggatgcc ggggccgtcg tcggcgacga cgacgcgggc gaggccccgg tccaccgagg
    33001 cgcggaccga gacggcgccc cccggcggcg tcgcggcgac agcgttgtcg acgaggttca
    33061 cgagggcgcg cttcacctgc tcggcgtccc cgcggaccgc cgggaggtcc ggagccgtct
    33121 cggcggcgac gcggactccg gccttcgtcc cctcgtacag cttcacgacc tgcgcgacga
    33181 ccgcgccgag gtcgagctcg ccgagccgcg tctccggaag ccgcgcgaag cggtggaacg
    33241 cgtcgacgag cgccgcgagc gagcgcacct cct
//
LOCUS       DAOONL010000032        31209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1234_length_31209_cov_20.076106, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000032 DAOONL010000000
VERSION     DAOONL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 31209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31209
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_31209_cov_20.076106"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLP50_07025"
     CDS             <1..929
                     /locus_tag="PLP50_07025"
                     /inference="COORDINATES: protein motif:HMM:NF027969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPA51339.1"
                     /translation="LVEELAMLDAAGMPFDRDLFLAGELTPVFFGSALNDFGVEPFLA
                     EFLELAPGPAPRESSAGSVDPAEPRFSGFVFKIQANMDPAHRDRVAFVRVCSGRFTRG
                     MEVRHARTGRTLKLNKPLQFLAQERTLVDEAWAGDVLGLWDSGALRIGDTIHEGPAVE
                     YAGIPRFSPELFCRVVLLDPLKRKQLKKGLDQLSEEGAVQVFYDPVRLERDPILGAVG
                     ALQFEVIQHRLRAEYGADVSYARLPHQIARWIENEGFDPRDFDDPGRCELLADGEGRP
                     LVLFESEWHFERAKKTNPKLTFAAAVQPARAR"
     gene            1060..1965
                     /locus_tag="PLP50_07030"
     CDS             1060..1965
                     /locus_tag="PLP50_07030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPA51340.1"
                     /translation="MEIPESFYRKLLDSLTDGVYFVDRDRRITSWNHGAQRITGYSGE
                     EVVGSYCWDNLLRHVDGEGRELCHAGCPLAQTVADGRDRETDVALLHKGGHRVPVKVR
                     AVAIRDASGEIIGAVETFQDETEHQETRQRAEELQRLAFLDPLTGVGNRRWAEAELAS
                     RLREAQRHGFTFGLLFVDIDRFKAVNDRHGHETGDAVLRLVATTLRHAARGHDFVGRW
                     GGEELVVLAAHCPGPAFGAVAERFRVLVESSRTPTPAGPVSVTVSIGAALARPDDTPE
                     TLVARADALMYRAKAEGRNRVVGET"
     gene            complement(1984..4026)
                     /locus_tag="PLP50_07035"
     CDS             complement(1984..4026)
                     /locus_tag="PLP50_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA51341.1"
                     /translation="MTSASVEGPPLRLLLLEDVQTDAELEVRELRRAGIPVEWTRVET
                     ETAFREALAVLDPDLVLADYSLPGWNGLAALEVVRSERPDLPFIVVTGALDEETAADC
                     IKRGADDYVLKERLSRLPHAVRTAREAWATRVARRRAEEELRLRNSALEAAADGILLT
                     DVDGRIVWTNPAFTQITGWPLEEILGRKPDVLKSSLQRDAFYRQMWETILAGGVWRGE
                     LYNRRKDGSIYLEEMTITPVRSDKGVITHFVAIKEDLTSRRRQEDLIRNLATRDPLTG
                     LPNQAMLREDIERAVAVDGACGTLVMIDLDRFALLNEALGHPAGDRLLIEVAERLSLD
                     LPRSGMLYRFGGDEFVVLLPESGLEEAGEIAERLRRTVDGIRFASENVVFDVSASLGL
                     APVEAARSASATLSLADAALRMAKEAGRNRWVVCGDDTAGRRAIDDIGRWAAQVKDAI
                     REGRFRLYAQRIVSLETGESHHEEVLLRMIDANGGVVAPGAFLPAAERFGLMPALDRW
                     VVSKAIELLRARPERRLFVNLSGTSLGDQALLAEVEALVGASGLPAGALTIEITETAA
                     IADIAGLQRWARRLKEAGCGFALDDFGTGFSSFAYLQSLPVDLVKIDGSFVRDLDTNA
                     TNRALTGAMVTVAHALGKTVVAEMVERAPVAEILKELGAEYGQGWYWGRPEPTEAA"
     gene            complement(4037..4462)
                     /locus_tag="PLP50_07040"
     CDS             complement(4037..4462)
                     /locus_tag="PLP50_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51342.1"
                     /translation="MQEAPDVLVVEDNPDDVELTLRALQRMHLTNPVRVARDGVEALE
                     VLLGDGTTRPPLPRVVLLDLKLPRVGGLEVLARLRQEERTRTLPVVVLMSSREEPDVK
                     KAYSLGVNSYVVKPVEFEKFVDAVGRVGQYWLLFNLSPA"
     gene            complement(4465..7848)
                     /locus_tag="PLP50_07045"
     CDS             complement(4465..7848)
                     /locus_tag="PLP50_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA51343.1"
                     /translation="MVRTPRAKGSAKDDRNPDASGAFRRLWPYAALLVLLAAGLVTVH
                     VGFARAARRDYRNTLTTLAGQQKAAIEAHLDQRIADARVFAAFPSVRALLASDAGLGD
                     EDSARLGVVLEAGRAEWGARSILLLGEDGAVRAGAGEQLPPGSAAVVAGVAARAVVVS
                     LVGDGEAARLLVAARVRAPGDAPRGWAVLVDDPSRSLWKILRREPIPSRTGEALLVST
                     EADGPVLLSPARHVVSDPTPPRELRLAADRTALEGLDGPDRFGELVDYRGETVWAAFR
                     RIDRTRWGLVVKIDRAEAMRSRAPGRLWQSLALVSLALAAAATFRAIRTSERLRTAAE
                     LQRRDERHRLVLEQVRDAVVWHRPGDGRILEANGAATELWGLSRDELLDRSLFDLLAA
                     EDVTEARLKMSGARRVGGLIRTRGKRRDGGLFPADISARTVVLEGEEILVSVVRDVSD
                     AEAALGQIRFLNRVLRTLTAVDQVLVEVHHRETVLRRTCEEIVSTGGFLAAWIGVPDA
                     EGRIAPAASAGAFAGYLEEVRITTDAGPLGRGPVGTAFREGRTVVIDDWESDPTVEPW
                     REAAARRGFRSGASCAVRTGETTIGVLTLYADRAGAFTPEAVLLLEDLARDLGLALEL
                     IEAGERRRSAEESLAASEARYRRLFEDNPGPMWVFDVASLRFLAVNEAAVSLYGYSRD
                     EFLGMTLREIRPAEDEAALRVDVQVPRTRIRSSGPWRHRTKDGRTLLVEVVTHDVTFE
                     ERPARLVLVNDVTERVRAEEKLRAFFDSGMVGAFLADASGRLTAANDEFLRIVGRTPA
                     DLEEGALSWRGLTPPEWIEVDVARSAEALERGVCTPYEKEYLRKDGSRVPVLVGFALV
                     GETREESVAFVLDLRERKAAEEEVRRLNSDLEARVERRTEELVAKSRELESFAYSVSH
                     DLRAPLRAIDGFSRMIEEDHAAGLDGEGRRLLGVVRDAARRMGRLIDDLLAFSRAGRQ
                     DLKRTRVDVGELVREVLREVLSEEERGRTDLEIGELPPVEADAPLLRQVFVNLLSNAV
                     KFSATRARRSIAIRGRREDARVVYDVVDNGVGFDMKFAGKLFGVFQRLHGREFDGTGV
                     GLALAERIVSRHDGSISAWAEPDRGARFTVSIPEAGGEK"
     gene            7982..8326
                     /locus_tag="PLP50_07050"
     CDS             7982..8326
                     /locus_tag="PLP50_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPA51344.1"
                     /translation="MKGPKVRVEELEVTGEKLLATVRELVRQGNVRRIVIRNAKGVTL
                     LEIPLVVGLAGAVLVPIWAAIGALAALLAKMTLVVERVQDAPEEPPPPETAAGSPKPR
                     RRSPGRRTAASD"
     gene            complement(8857..9612)
                     /locus_tag="PLP50_07055"
     CDS             complement(8857..9612)
                     /locus_tag="PLP50_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPA51345.1"
                     /translation="MTEPPFDRWLEDLERRHLAELSFAQVRKGLQAVSSLYVERRGRI
                     GSGAVFEGEGKRAAFALFYGPLHHLVVERIVASVGAAEPPLSRVLDLGCGTGAAGAAW
                     ALAADAEVFGVERSGWAASEARRTYAALSLRGRATAGDLLRERLPGRGAAILLGWVVN
                     ELGDPSRGTLLPRLLEAARRGARVLVVEPIAARPVPWWGEWEQAFAADGGRGDLWRFS
                     APLPPLVARLDRAAGLDHRVLTARSLYLPGHQD"
     gene            complement(9635..10861)
                     /locus_tag="PLP50_07060"
     CDS             complement(9635..10861)
                     /locus_tag="PLP50_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51346.1"
                     /translation="MSVHATRFRLAAAAAFAVLAAPLAPSPADACTTAIVAPSASRSG
                     RPMLWKNRDVDGSDNQVVYLADGRYPYVGVVNRGDAAGLQVWAGINSTGFGVMNSASY
                     NLESGDTAGEGTFMKLALQSCATVDDFEALLARSNSGGRDVSANFGVIDAKGGAAVFE
                     TSAKGYVRVDAAPSGDGARGLLVRSNYSHSGTKGSGSGFLREARAIELLGSSTAGGLD
                     LATILRAARDVGNAGIGQTGEESGPPLVYTGDSICRLDTVAAIVLEAPAPGEGPEAGA
                     LWIVPSLPLAGAAVPVFPHAGAVPAPLAAGRGPAPLAARALELRKRLFPDTRGEKKRY
                     MDRRALAALKARSLPGLLAAEEATRAEVEAAREAWRRSRPAPADAAKLSASAAARALA
                     AGEAAVAAVAKELGEP"
     gene            10991..12199
                     /locus_tag="PLP50_07065"
     CDS             10991..12199
                     /locus_tag="PLP50_07065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPA51347.1"
                     /translation="MQDRPRRESFLPFSRPSIGEEEIAGVVEALRSGWITTGPRTARF
                     EEELAAFNEVPRCLALLSATTGLELALGALGIGEGDEVITTAHTFVCTVSNVVLRGAV
                     PVLVDVEADTMNLDAERIEERITPRTKAILPVHYGGLPCRMDRIWAVAERHGLAVVED
                     AAHTMGARYAGRRIGADPRSAFSVYSFHPNKNLTTIEGGAIAFHDLRHEAYLKLQRFH
                     GIARGAGTPYPEGSPLYDVPMPGRKSNFTDVQAAVGLAQLPKLDAFAARRRELARLYL
                     SLLAEVPEVELPADGDAEREHCWNLFVLRNRPEATGLSRDELTAALKAENVGTGIHWQ
                     GLFAFSFYRDYFARHPEGLPKDGLPHATRTTENLMSLPLFPAMTEEDVLDVVSALRRI
                     VAAGRRRARS"
     gene            complement(12271..13242)
                     /locus_tag="PLP50_07070"
     CDS             complement(12271..13242)
                     /locus_tag="PLP50_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA51348.1"
                     /translation="MVVPGEAAAFAVGRSAAWCRLVERATRVAPLGVTVLIRGETGSG
                     KDVVARLIVALGNREGAPFVAVNCAALPDTLVESELFGHARGSFSGATEARRGLFEEA
                     HRGTLFLDEIGALPLSAQAKLLRVLEEGRVRRVGENRVTDVRVRVLAATSLDMEAAIG
                     RREFREDLYYRLSALELLVPPLRERPEDIPLLAEHFLRKLREQGGPERTLSPEALDLL
                     TGYPFPGNVRELKHAVEQAAVFGDGPILEPTDFRALWGRSQMLLPASEPRGGRSPIVD
                     VTSARLEEALRRTGGNRLEAARLLGISRSSFYRILRRLPAAEAPQPD"
     gene            13291..14232
                     /locus_tag="PLP50_07075"
     CDS             13291..14232
                     /locus_tag="PLP50_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPA51349.1"
                     /translation="MSNVRLLLLEPDPARSARIARSFEELRTPVTVAASVEEVALAAA
                     SGPVVVAASGLGGGGAARLRALREDPRTASLAVLAVAPRGAGSVAALRAGADDVVPWP
                     SPAPVVRARLRGLFADARARAESHRTVAAFAGLLEAFEAREPHRIEHSARVGRLAAGM
                     GRRAGLSEAEIERVRVGGVLHDIGTVAIPDGILYKRERLTDGELAVVKSHPVIGFSLI
                     RDVPALEPYGPFVLRHHERIDGSGYPDGLRGAEIPLPVQLVALADAFDAMTSSRPYRA
                     VRDDGQTLSVLEGEARRGQWDRALFPLLVAEAEAQGG"
     gene            14381..15871
                     /gene="ahcY"
                     /locus_tag="PLP50_07080"
     CDS             14381..15871
                     /gene="ahcY"
                     /locus_tag="PLP50_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPA51350.1"
                     /translation="MSTVLDPKVAPASSARPKFKVADLSLSELGRKEIRLAEQEMPGL
                     MALRERHRGEKPLAGERVMGSLHMTVQTAVLIETLVELGADVRWVSCNIFSTQDHAAA
                     AVVVGRPESGGTPESPKGTPVFAWKGETLEEYWWCTREAIEWPDGSGPTLIVDDGGDA
                     TLLVHKGLEFETAGKVPAFDPKNDPEEWGVILELIRTELAKDGKVFSRLAKAIRGVSE
                     ETTTGVHRLYQMEKDGTLLFPAINVNDSVTKSKFDNVYGCRHSVVDGLNRATDVMLGG
                     KVAVVLGFGEVGKGCAEALRGQGCRVIVTEIDPICALQAAMQGYEVKTLEDVVATADV
                     FITATGNLDVITAEHMAKMKDKAIVGNIGHFDNEIDMAGLKKVPGVTRINIKPQYDEF
                     RFPDGHSVMILAEGRLMNLGCATGHPSFVMSSSFTNQVMAQIALAKNRGEYGKKVYVL
                     PKKLDEEVARLHLAHLGVKLTTLTPKQAGYLGVPAEGPYKPDHYRY"
     gene            complement(15956..17440)
                     /locus_tag="PLP50_07085"
     CDS             complement(15956..17440)
                     /locus_tag="PLP50_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPA51351.1"
                     /translation="MRAGGALLLLALPLWLGCATAPRATILYVTDAHEIAPVVDRHGE
                     RGGVARLKTVVDRLGAGGPDALLVFGGDLAGGTLFGGVFRGEPMVDALGRVGVDLATF
                     GQHEFDFGPAHAERLVRRSAFPWITSNLVDEAGRPFAALPTRRLVRVGGLTVGFLGLT
                     DDLGTTTPHRAVRQTDLVEAVAREAAALRREGAEAIVVLTQAGFEVNRRLLAEVPEVD
                     AVLTEEESETVSVVRWFDGRPVAAPSGNLGSVVELTLERSGGRISSRVAVHPVDVSVA
                     PDPELSEMEKELMARLERELAAPAGALARPLDETGSHERETPLGSLVADAFRAGTGAD
                     VALVPGSSLRAPLPAGPLSMRHVVSALPFGNRVVTVELAGAELRAVLERSLAAVGRRT
                     GGLLQLSGMTLEADLGAPAGRRLLAVRVADAPLRDDATYRVAVSSFLAAGGDGHAELA
                     AARRLGGVEPLDADLLAEHLRRLSRSGPAEPPTLGRMSFAPSGR"
     gene            complement(17481..18422)
                     /gene="arcC"
                     /locus_tag="PLP50_07090"
     CDS             complement(17481..18422)
                     /gene="arcC"
                     /locus_tag="PLP50_07090"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPA51352.1"
                     /translation="MRVVVALGGNALLKRGEPMTAENQRANVKRACEALAPVANDNEI
                     VISHGNGPQVGLLALQQSAYAHVEPYPLDVLGAQTEGMIGYVIEQEMGNLLPFERPFA
                     TILSMTEVDPADPAFRNPTKPIGPVYSKEDADRMAAEKGWTFKADGNGWRRVVPSPHP
                     KRIFEIRPVKWLLEKGTVVIFAGGGGIPTMYGPDRKLQGIEAVIDKDLASSLLAREVG
                     ADAFVMATDADAVYVGWGTPEARALKTVSIADLKKYSFPAGSMGPKVQAACEFAVATG
                     KAACIGALEDTARMLRGEAGTTVVRDDVEPEYWPRKG"
     gene            complement(18582..19580)
                     /locus_tag="PLP50_07095"
     CDS             complement(18582..19580)
                     /locus_tag="PLP50_07095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPA51353.1"
                     /translation="MSFNLKNRHFLTIHDFTPKEILYLLDLARDLKRAKYAGTEVKRL
                     GGKNVALIFEKDSTRTRCAFEVACYDQGANVTYLGPSGSQIGKKETMKDTARVLGRMY
                     DAIEYRGYGQAIVEELAKWAGVPVYNGLTDEFHPTQILADLLTMREHSDRPLGQVSYA
                     YLGDARNNMGNTLMLGGALMGMDVRLGAPKHLWPVEELVAKANELAKASGGKVTLTED
                     PKAAVKGVDFVHTDVWVSMGEPDSVWAERIKLLLPYQVNAALMAATGNPKAKFLHCLP
                     SFHNRDTKIGEEIYKKFGIEAMEATDDVFESPASVVFDQAENRLHTIKAVLVATLA"
     gene            19756..20940
                     /locus_tag="PLP50_07100"
     CDS             19756..20940
                     /locus_tag="PLP50_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA51354.1"
                     /translation="MKRDYAISGTTLMLAAALGGALLTQAACKKSGPDIKVGVIAELS
                     GDISEIGDACKKAAELAVTEINEAGGVEVAGTKHLLRLVIEDSQGAAEPAAEAAKRLI
                     DQEKVLAIVGPNASFGAVPAAQVAAEKKTLLISPWGTAPRVTLDDTGAPRTWVYRTCF
                     TDAFQGSALAKFAAAYMKATKAAVLYNPASEAPKSQAELLKKEFEGRGGQVVAFETYA
                     AGDKDFSAQWKRIAEAAPDVVFLPSYYGEIPEQIRQARAAGVKAPFLGSDTWTNSELL
                     KSAAEVDGAFFCAHYNPDAMEDKVGIFRGAYENRYDKEVPDDGAALTYDTLYLLKQAL
                     ETATSDDREAVREAFSKISSFDGVTGRIVFKGGAPDPVKSVVIKQFKAGKISFVTNID
                     PD"
     gene            21154..22155
                     /locus_tag="PLP50_07105"
     CDS             21154..22155
                     /locus_tag="PLP50_07105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA51355.1"
                     /translation="MKYTQLGRTGLVVSRLALGCMSYGDPRWRPWVLSEEDARPFFRR
                     ALEAGINLFDTADMYSLGASEELTGRLLREMGRRDELVIATKVHFPMGPGPNMSGLSR
                     KHVVQACEASLRRLGVETIDLYQIHRFDPATPVAETLEALDLLVRQGKVRYVGASSGE
                     AFRLAKALATADLRGYPRFVSMQNHYNLLYREEEREMLPLCAEEGLGVLPWSPLARGL
                     LAGTRATPADREATPRAASDDYARQLYDHPSDADVIDATRSVAAARGVPMARVALAWL
                     LSRPGVTAPIVGATKLAHLEDALAALDLALSPEETAALEEPYRPHAVRGFAFSPPAR"
     gene            22200..23114
                     /locus_tag="PLP50_07110"
     CDS             22200..23114
                     /locus_tag="PLP50_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA51356.1"
                     /translation="MRVFVTGATGHIGSAVAAAFARAGHETCGLVRDPQKTASLEAAE
                     VVPVLGTMEEPDAWREVAGRCSVLVHCAVDSSDRSWDLHRAALGALRDAARDRVDRVL
                     LATSGVWVYGDTGEAAVDESTPLDPPTFSAPRPAVDAAVLSWNGAGLRTLVIRPGCVY
                     GGAGSLTADWFESASRDGAARVVGDGTNRWAMVHRDDLADLYVRAAESGLAGEVLNAT
                     DRSRSTVLECAIAASRAAGAEGRVEHLPVEQAVAAMGPFATGLALNQHVASWKAVRRL
                     GWTPRHAGFVDEAPRLFAAWRASRTEAA"
     gene            23111..23491
                     /locus_tag="PLP50_07115"
     CDS             23111..23491
                     /locus_tag="PLP50_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA51357.1"
                     /translation="MSAPAIGSIGWLDLTVEDAPRLRDFYAAVTGWRPEPVPMGDYSD
                     WAMIDPSSGQARAGVCHARGGNAGLPAAWLVYVVVADLDASVAAATERGGEVLLGPKA
                     MGESRYAVLRDPAGAAFALWQNGA"
     gene            23634..25526
                     /locus_tag="PLP50_07120"
     CDS             23634..25526
                     /locus_tag="PLP50_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA51358.1"
                     /translation="MSVLLRPPEFDHYSVSRVLGEGGMGIVFLAEDRRTHLPVAVKVM
                     SRSLTDPELQARFMKENQILASLNHRNIVRCYEITRSREGLPTIVMEYVKAVDFGAFE
                     GRPFPELIPLMVQAAMGLAYLKERNILHRDLSPNNIMVTLQDERRLVKILDFGVAKVL
                     QEGSGGELTQTGEFLGKLAYASHELLTFGQVDFRSDIYSLGVIFFRLLTKRRPINVQN
                     SRNYLEWVMAQENRGPIDFAAPEGNPAIPDSLQALVLKMLAKKSEDRPQGYEEIIDAL
                     VAAQSEAEKSGLVPDPETVSTLPSPEARSSISGSAGTPASPAGSSPTPGGSGAAGLFD
                     DGAFPFTEAETKAPSSSPSLAGSRLPAPAPAPKATPASQMPDWLEQADYNEQMQVISE
                     SKQGIPRSPVVHPAFGGESRPRPVRRDTESIRVRQKEASSRKRTTVLVSLLVVAILGG
                     GAWAGWTFYVKPNLPSSSPEALALPAPPDPASVAPPTAPETVLPTAVPEPAKTVSRAA
                     KLGSAERRLVDANVAGFYASADTMGRGLSIILEFRQPANVSGLKSARIVSATDASGRN
                     VPGVAGATAEIRLNLNDDPTSLVRVRLFAVGENAAAAAASLRSISVEIGSATMRARFD
                     PRVLSR"
     gene            25588..26067
                     /locus_tag="PLP50_07125"
     CDS             25588..26067
                     /locus_tag="PLP50_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51359.1"
                     /translation="MRRSSAVPPTVTFWVAQAGDVPTLLPMMRALWEHEGIPFDEGLV
                     EPPLRELLSEPALGRVWLALAGGAVAGYAMGTWGFSTEQGGRFLLLDELFVLPAFRGT
                     GIGRAALAFVEEAARGAEGRAVRIEVAEENDGARRLYREAGYVDPRRLFLAKRLTER"
     gene            26127..27794
                     /locus_tag="PLP50_07130"
     CDS             26127..27794
                     /locus_tag="PLP50_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HPA51360.1"
                     /translation="MPLRRIEIEGFRGIRRMTLDLDDTTALIGENSCGKTSVLDALEL
                     AFGHRGARPAFSPLDFHLPVGTAAPVERISMRFTFAGEGGRETVAELTASRTPDGSPA
                     VATSRLTDRDGRDLSPGDEEAVEAFRRRHPVLRVRFNRPPRVAPVEIDLETPATGPEA
                     PAPAVVPPAPGEAGEGEVTRKLRRTVESAYRKFSGSWRPHPEELREPIAAARALAERW
                     VERLRLPPRAPGGRTTEILAETPVLLGATDPLRAESREGTGMQSLALLMLLGAVLDAE
                     NGSPVEAGAEPLVLIEEAEAHLHPILAARVFGLIDRVAGQKILTTNSGDLLASVPLHA
                     IRRLVRNGGGVAVHGLAEGALTVDEMRRVGYHIRVNRASSVFARCWLLVEGETESWLL
                     PEIARLIGHDLPAEGVRCVEFAQCGIAPLVKMARGLGIEWHLLADGDDAGRHYVRAAR
                     SFLQGEPDRERVTGFRERDVEHHLWGAGYAGVYRAAAQRAGVFPSLFGRRRIPTTAII
                     DRALKAHGKPRMALEVAEAMAAPDSPGVPRLLASVVEKAVRLARFST"
     gene            complement(27819..28517)
                     /locus_tag="PLP50_07135"
     CDS             complement(27819..28517)
                     /locus_tag="PLP50_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPA51361.1"
                     /translation="MRGLLFFDLDDTLLDHAGAEARAQLETFEGRRDVFGAVAFDEWL
                     AAYRRANGRLWAAYGRGEIGREELHRRRFADPLGELGCDPEAADETGAFYLERYRGAW
                     RLNEGAEELLAAAAKEGEVGILSNGFRELQRAKLARFRLERWVKHVVLSEEAGAMKPA
                     REIFDAAVRSACGGDGADGRRKLYLGDHYETDAVGAKGAGWLPVLYNPSRRPLPAPVL
                     NVARLTDAIPLLAG"
     gene            complement(28665..>29474)
                     /locus_tag="PLP50_07140"
     CDS             complement(28665..>29474)
                     /locus_tag="PLP50_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA51362.1"
                     /translation="EACGRYGARAFAWCLMPNHIHLALQTGSAPISRLVHDVHSRYAQ
                     YFNRRWERSGHLFQGRFQGIIVDREGYLLEVVRYIHRNPVKARLVARPEEFPWSSHGA
                     YLSTGPSWLASGEVLGLLAGGRPKARRLFQEFVTGSSAGRYDPDGARFGAVVGGEDFV
                     RTALAGAGREDLVRRTLTVEAVVKAVAAHEEVSVDELTGPSRLRSLSRVRSLCALLGR
                     EYGGIPFARTARFFGRDASTISRDVAFLERRLAEDPAEARRLAELRRVLAE"
     assembly_gap    29475..29574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29602..30045)
                     /locus_tag="PLP50_07145"
     CDS             complement(29602..30045)
                     /locus_tag="PLP50_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51363.1"
                     /translation="MGAPPPAPPLLPPPPPPRPAPPPAFALPVVEPACLRWQRVYLGA
                     MAAIYLACVVGGALLLVFRQEVADWPPRDEPWEIVLTGGLLLGLGVPFLALYVAGYFL
                     PRRPWAWVAHLVLIAFGLTSCCTMPAAIPLLVAFLRRDTQAWFGR"
     gene            complement(30047..30316)
                     /locus_tag="PLP50_07150"
     CDS             complement(30047..30316)
                     /locus_tag="PLP50_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HPA51364.1"
                     /translation="MITGVVIGEITATIRHPFFEGKKMLVVARTGADGAETGDYVIAI
                     DAIGVGPGDPVLVLDEGNGARQVVGSSTAPVRSVVVGFLDRVDVV"
     gene            complement(30313..30597)
                     /locus_tag="PLP50_07155"
     CDS             complement(30313..30597)
                     /locus_tag="PLP50_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HPA51365.1"
                     /translation="MLLGRVIGRVVPCVVTPGLEGVPMLLLQPLGKDGAPKGKTLVAA
                     DPTRMAGEGELVAYEGGREAALLLDPWFVPVDHAVVGIVDALHLPAKEPA"
     gene            complement(30597..>31209)
                     /locus_tag="PLP50_07160"
     CDS             complement(30597..>31209)
                     /locus_tag="PLP50_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HPA51366.1"
                     /translation="MKRAAALAVLDFAQIPAGLDATDAMLKKAPVAFFRSGTVTAGRY
                     LTLIGGTPASVEEALAEGLARAEGHLLDHLYLADVHPRLLDALEGRRGEDGTGALLIL
                     ETATASSVVLACERALKGTLVDLVEVRLSDAGLAGKGLALFRGDLHELEAAVEIVTSA
                     LPPAAGLSYRVLARPHDAFAREAALGTAFAASRFVDLGGEEAS"
BASE COUNT         4315 a        11597 c        11021 g         4176 t
ORIGIN      
        1 agctcgtcga ggagctcgcg atgctcgacg cggccgggat gccgttcgac cgcgatctct
       61 tcctcgcggg cgagctgacc cccgtcttct tcgggagcgc gctgaacgac ttcggcgtcg
      121 agccgttcct cgccgagttc ctcgagctgg cgcccggccc ggcaccgcgg gagtcgagcg
      181 ccgggtcggt cgacccggcc gagccgcgct tctccggctt cgtcttcaag atccaggcga
      241 acatggaccc ggcccaccgc gaccgcgtcg ccttcgtccg cgtctgctcg gggaggttca
      301 cccgcggcat ggaggtgcgg cacgcgcgca ccggacggac gctcaagctg aacaagccgc
      361 tccagttcct cgcgcaggag cgaacgctcg tcgacgaggc gtgggccggc gacgtcctcg
      421 gcttgtggga ctcgggggcc cttcggatcg gcgacacgat ccacgagggg cccgccgtcg
      481 agtacgccgg catcccccgc ttctcccccg agctcttctg ccgcgtcgtc ctcctcgacc
      541 cgctcaagag gaagcagctg aagaaggggc tcgaccagct ttcggaggag ggggccgtcc
      601 aggtcttcta cgacccggtc cgcctcgaac gggacccgat cctgggcgcg gtcggcgcgc
      661 tccagttcga ggtgatccag caccggctgc gggccgagta cggagccgac gtctcgtatg
      721 cgcgactgcc acaccagatc gcccgctgga tcgagaacga gggcttcgac ccgcgcgact
      781 tcgacgaccc gggccgatgc gagctgctcg ccgacggcga ggggcggccg ctcgtcctct
      841 tcgagagcga gtggcacttc gagcgggcga agaagacgaa tccgaagctg acgttcgccg
      901 ccgccgtaca gccggcccgg gcccggtagc cggctccgcc cggaccgccc cgcctccccc
      961 gtccctctcc cgagcctccc cggcggacgc gaacgttgcg cggggagagc gcccggctcc
     1021 acaatcagga cctcggacgg gctggatcgg aggcggacgg tggagatccc cgagtccttc
     1081 tacaggaagc tgctcgattc gctcacggac ggcgtctact tcgtcgaccg agaccgccgc
     1141 atcacctcgt ggaatcacgg cgcccagcgg atcaccggct attccggaga ggaggtcgtc
     1201 ggctcgtact gctgggacaa cctcctgcgc cacgtcgacg gcgagggacg cgagctctgc
     1261 cacgccggct gcccgctcgc ccagaccgtc gcggacggac gggaccggga gaccgacgtc
     1321 gcgctcctcc acaagggcgg gcaccgcgtc ccggtcaagg tgcgcgcggt cgcgatccgc
     1381 gacgcctcgg gcgagatcat cggagcggtc gagacgttcc aggacgagac ggagcaccag
     1441 gagacgcgcc agcgcgccga ggagcttcag cgcctcgcgt tcctcgaccc gctgacgggc
     1501 gtcggcaacc ggcgctgggc cgaggcggag ctcgcctcgc gcctccggga ggcccagcgg
     1561 cacggcttca cgttcggcct cctcttcgtc gacatcgacc gcttcaaggc ggtcaacgac
     1621 cgtcacgggc acgagacggg cgacgcggtc ctccggctcg tcgcgacgac gctgcggcac
     1681 gccgcgcgcg ggcacgactt cgtcggccgc tggggaggcg aggagctcgt cgtcctggcc
     1741 gcccattgcc ccggtccggc gttcggcgcg gtggcggaga ggtttcgcgt cctcgtcgag
     1801 agctcccgga cgccgacgcc cgcgggcccg gtctccgtga ccgtctcgat cggcgcggcg
     1861 ctcgcccgtc cggacgacac gcccgagacg ctcgtcgccc gcgccgacgc cctgatgtac
     1921 cgggcgaagg ccgagggccg caaccgcgtc gtcggagaga cctgagcccc gcggccgctc
     1981 tcctcaggcc gcctcggtcg gctccggcct cccccagtac cagccctgcc cgtactccgc
     2041 cccgagctcc ttcaggatct cggcgaccgg cgcccgctcg accatctcgg cgacgacggt
     2101 cttcccgagg gcgtgagcga ccgtgaccat cgcgccggtc agggcccggt tcgtggcgtt
     2161 cgtgtcgagg tcgcggacga acgagccgtc gatcttcacg aggtcgacgg ggagcgactg
     2221 gaggtaggcg aacgaggaga agccggtccc gaagtcgtcg agggcgaagc cgcagcccgc
     2281 ttccttcagc ctccgggccc agcgctggag gccggcgatg tcggcgatgg ccgccgtctc
     2341 cgtgatctcg atcgtcagcg cgccggctgg caggccgctc gcgccgacca gcgcctcgac
     2401 ctccgcgagg agcgcctggt cgccgaggct cgtgccggag aggttcacga agagccggcg
     2461 ctccgggcgg gcgcggagga gctcgatcgc cttcgagacg acccaccggt cgagcgccgg
     2521 catcagcccg aagcgctccg cggccggcag gaacgccccc ggagcgacga cgcccccgtt
     2581 cgcgtcgatc atccggagga ggacctcctc gtggtgggac tccccggtct cgagcgagac
     2641 gatcctctgg gcgtagagac ggaaccgccc ctcccggatc gcgtccttca cctgggcggc
     2701 ccagcgcccg atgtcgtcga tggcgcgccg gccggccgtg tcgtcaccgc agacgaccca
     2761 gcggttccgc ccggcctcct tggccattcg caaagcggcg tcggcgaggg agagcgtcgc
     2821 cgaagcgctc cgggccgcct cgaccggggc gaggccgagg ctcgccgaga cgtcgaagac
     2881 gacgttttcc gaggcgaacc ggatcccgtc gaccgttcgc cgcagccgct ccgcgatctc
     2941 tccggcctcc tcgaggcccg actccgggag aaggacgacg aactcgtccc cgccgaagcg
     3001 gtagagcatt ccgctccggg gcaggtcgag cgagagacgt tcggccacct cgatcaggag
     3061 gcggtcgccg gcaggatggc ccagggcttc gttcaggaga gcgaaccggt cgaggtcgat
     3121 catgacgagc gttccgcacg ctccgtccac ggccaccgcg cgctcgatgt cctcgcggag
     3181 catcgcctgg ttcggaagcc cggtcagggg atcgcgcgtc gcgaggttcc ggatcaggtc
     3241 ctcctgcctc cggcgggagg tcaggtcctc cttgatcgcg acgaaatgcg tgatcacgcc
     3301 tttgtcgctc cggacgggcg tgatcgtcat ctcctcgagg tagatcgacc cgtccttccg
     3361 gcggttgtag agctccccgc gccagacccc gccggccagg atcgtctccc acatctgccg
     3421 gtagaaggcg tcccgctgga gactcgactt caggacgtcc ggcttccgcc cgaggatctc
     3481 ctcgaggggc cagcccgtga tctgggtgaa ggcgggattc gtccagacga tccgcccgtc
     3541 cacgtcggtc aggagaatcc cgtcggcggc ggcctccagg gccgagttgc ggagccggag
     3601 ctcctcctcg gcccgtcgcc gggcgacccg cgtcgcccag gcctcgcgcg ccgtgcggac
     3661 cgcgtgaggg aggcgcgaga gccgctcctt caggacgtag tcgtccgctc cccgcttgat
     3721 gcagtcggcg gccgtctcct cgtcgagcgc ccccgtcacg acgatgaatg gaaggtcggg
     3781 gcgctcgctt cggaccactt cgagcgcggc caggccgttc caccccggga gcgagtagtc
     3841 cgcgaggacg aggtcggggt cgaggaccgc gagcgcctca cggaaggccg tctccgtctc
     3901 gaccctcgtc cactccaccg ggatgccggc ccgccggagc tcccggacct ccagctctgc
     3961 gtcggtctgg acgtcttcga gcaggaggag cctgaggggc ggaccttcga ccgaagcgga
     4021 cgtcatggca gagccctcac gccggagaca gattgaaaag gagccagtac tgccccaccc
     4081 tcccgacggc gtcgacgaac ttctcgaact cgacgggctt gacgacgtag ctgttgacgc
     4141 cgagggaata ggccttcttc acgtccggct cctcgcgcga ggacatgagg acgaccacgg
     4201 ggagagtccg ggtccgttcc tcctggcgga ggcgggcgag gacctcgagg ccgcccacgc
     4261 gggggagctt gaggtcgagc agcacgaccc ggggcagcgg cggccgggtc gtgccgtcgc
     4321 cgaggaggac ctcgagcgcc tcgaccccgt cgcgggcgac ccgcacggga ttcgtcaggt
     4381 gcatccgctg cagggcgcgg agggtcagct cgacgtcgtc ggggttgtcc tcgacgacga
     4441 gaacgtccgg agcttcctgc accgtcattt ctctcctcct gcctcgggga tcgacaccgt
     4501 gaaccgggcg ccacgatccg gctcggccca ggccgagatc gaaccgtcgt gccgggagac
     4561 gatcctctcc gcgagcgcga gcccgacacc ggtgccgtcg aactccctcc cgtggaggcg
     4621 ctggaagacg ccgaagagct tccccgcgaa cttcatgtcg aaccccacgc cgttgtccac
     4681 gacatcgtag accacccggg cgtcctcgcg ccgcccccgg atcgcgatac ttcgccgggc
     4741 tcgcgtcgcg ctgaacttca cggcgttcga gaggaggttc acgaagacct gccggaggag
     4801 gggagcgtcc gcctcgacgg gcggtagctc gccgatctcg aggtcggttc gcccgcgctc
     4861 ttcttcggac aggacctccc gcaggacctc ccggacaagc tccccgacgt cgacgcgcgt
     4921 cctcttcagg tcctgccttc cggcccggga gaacgcgagg aggtcgtcaa tgagcctccc
     4981 catccgccgc gcggcgtccc ggaccacccc gagaagccgc cgcccctccc cgtcgagccc
     5041 cgcggcgtgg tcctcctcga tcatgcggga gaaaccgtcg atcgcccgca gcggcgcccg
     5101 gaggtcgtgg gaaaccgagt aggcgaagct ctcgagctcc cgggacttcg cgacgagctc
     5161 ctcggtcctt cgctcgacgc gcgcctcgag gtcggagttc aggcggcgga cctcctcctc
     5221 ggccgccttt cgctcccgga ggtcgaggac gaacgcgacc gattcctcgc gggtctcgcc
     5281 gacgagcgcg aagccgacga ggacgggaac gcggctgccg tccttccgga ggtactcctt
     5341 ctcgtagggg gtgcagacac ctcgctccag ggcctccgcg gaccgcgcca cgtcgacctc
     5401 gatccactcc ggcggcgtca gaccccgcca ggagagcgcc ccctcctcca ggtcggccgg
     5461 ggtgcgtccc acgatccgga ggaactcgtc gttggccgcc gtgagccgcc cgctcgcgtc
     5521 cgcgaggaac gcgccgacca tcccggagtc gaagaaggcg cggagcttct cctccgcgcg
     5581 gacacgctcc gtcacgtcgt tcacgaggac gagccgggcc ggcctctcct cgaaggtgac
     5641 gtcgtgcgtg acgacctcga cgaggagcgt ccgtccgtcc ttcgtccggt gccgccacgg
     5701 tcccgacgat cggattcgcg tccgcgggac ctgcacgtcg acccggagag ccgcttcgtc
     5761 ctccgccggc cggatctcgc gaagcgtcat cccgagaaac tcgtcccgcg agtagccgta
     5821 gaggctgacg gcggcctcgt tcacggcgag gaaccgaagg gacgcgacgt cgaagaccca
     5881 catcggtccg gggttgtcct cgaagagcct ccggtagcgg gcttcgctcg cggcgagcga
     5941 ctcttccgcg ctccggcgcc gctcgccggc ctcgatcagc tcgagcgcga ggccgaggtc
     6001 ccgcgccagg tcctcgagga ggaggaccgc ctccggggtg aaggccccgg cgcggtccgc
     6061 gtagagcgtc aggactccga tcgtcgtctc acccgtccgg accgcgcagg aggctcccga
     6121 gcggaaccct cgccgcgccg ccgcctccct ccacggctcg acggtggggt cgctctccca
     6181 gtcgtcgatg acgaccgttc gcccctcgcg gaacgcggtc ccgaccggcc cccgcccgag
     6241 cggccccgcg tcggtcgtga tccggacctc ttcgagatag ccggcgaagg cgccggccga
     6301 ggcggccggg gcgatccgtc cctccgcatc cggaacgccg atccacgctg cgaggaagcc
     6361 acccgtcgag acgatctcct cgcacgtccg gcgaaggacg gtttcgcggt ggtggacctc
     6421 gacgaggacc tggtcgaccg ccgtcagcgt ccggaggacc ctgttgagga agcggatctg
     6481 accgagagcc gcttcggcgt cggagacgtc ccggacgacc gagacgagga tctcctcgcc
     6541 ctcgaggacg acggtccggg cagagatgtc cgccgggaag aggcctccgt cccgccgctt
     6601 cccccgcgtc cggatgaggc cgccgacgcg gcgcgcgccg gacatcttca gccgggcctc
     6661 cgtcacgtcc tccgcggcga ggaggtcgaa gaggctccgg tcgaggagct cgtcgcggga
     6721 caggccccag agctccgtcg ccgcgccgtt cgcttcgagg atccggccgt ctccgggccg
     6781 gtgccagacg accgcgtcgc gcacctgctc caggacgagg cggtgccgtt cgtcccggcg
     6841 ctgcagctcg gccgccgtcc ggagccgctc cgacgtccgg atcgcccgga aggtcgcggc
     6901 ggccgcgaga gcgagggaga cgagggcgag ggactgccac aggcggcccg gcgcgcggct
     6961 ccgcatggcc tcggcccggt cgatcttcac gacgaggccc cagcgcgtcc ggtcaatccg
     7021 ccgaaacgcg gcccagaccg tctcgccgcg gtagtcgacg agctcgccga agcggtcggg
     7081 gccgtccagc ccctcgagtg cggtccggtc cgcggccagg cgcagctcgc ggggcggtgt
     7141 cggatcggat acgacgtggc gcgcggggga gaggaggacg gggccgtccg cctccgtcga
     7201 gacgaggagc gcctcccccg tgcgggacgg gatcggctcg cgccgcagga tcttccagag
     7261 cgacctcgaa gggtcatcga cgaggacggc ccagccgcgc ggtgcgtcgc ccggcgcgcg
     7321 gacgcgcgcc gccacgagga gacgcgcggc ctccccgtcg ccgacgaggc tgacgacgac
     7381 cgcccgcgcc gcaacgccgg cgacgaccgc ggcagagccg ggcggcagct gctctcccgc
     7441 gccggcccgg acggccccgt cctcgccgag gagcaggatc gagcgtgccc cccactcggc
     7501 ccggcccgcc tcgaggacca ctccgaggcg ggccgagtcc tcgtcgccga gcccggcatc
     7561 cgaagccagg agggcgcgga cgctcgggaa ggccgcgaag acgcgggcgt ccgcgatgcg
     7621 ctggtcgagg tgagcctcga tggcggcctt ctgctggccg gccagcgtcg tcagtgtgtt
     7681 ccgatagtcc cgtcgcgccg ccctcgcgaa cccgacgtgg acagtcacca ggcccgcagc
     7741 gaggaggacg agcagggcgg cgtacggcca gaggcggcgg aacgcaccgg acgcgtcggg
     7801 gttccggtcg tccttcgccg agcccttcgc tcgcggggtt cgaaccacgg ccgagcccac
     7861 ctgctcctcc gggacgagag taccgcgata cggccatccc tgcgccgatt ccggcccgtt
     7921 tgggcaccct cggcgcgaac ccgtccgcgg cccccgccgt acctcgtcgc ggaggcacac
     7981 catgaagggt ccgaaggtcc gcgtcgaaga gctcgaggtg accggcgaga agctgctcgc
     8041 gacggttcgg gagctcgtcc gccaggggaa cgtccgtcgc atcgtcatcc gcaacgcgaa
     8101 aggggtgacg ctcctcgaga tcccgctcgt cgtcgggctc gcgggggccg ttctcgtccc
     8161 gatctgggcg gcgatcggag cgctcgcggc gctcctcgcg aagatgacgc tcgtcgtcga
     8221 gcgcgtccag gacgccccag aggagccgcc cccgcccgag accgccgccg gaagcccgaa
     8281 gccgcgccgt cgttcgcccg ggcgccggac cgccgcgagc gactgaggcg ggcgcgtccg
     8341 ccgccgaacc ggtcccggcc ccaggcgcgc ttcccgagcc tcccggcgcc gcgtcgcgcc
     8401 gaccgggcac gaaacggtag ctgcgacgtt cgttccccgt gcctcgcgat cgagggcatc
     8461 gccgggctgt tcgtcggaga ctcgcccgtg gcgctggccg aggagaccct ccgccggctc
     8521 gacgtgagcc ggggcaacgc ccggcggatg tcctccatca tcgacgtcct cctgcgcttc
     8581 tcccgcctgg gccggtacga gcggcagcgg gaccgggtcg acagggcgga gctcgcgcgg
     8641 tcggccctcg aggaggcggt cggcgacgcc tccactcggg cacggatcga ccttcggatc
     8701 gcggagcggc cttccgccga gtgcgcgagg gcgggggcgc cgtcttccgt ttctccgtgc
     8761 cctcggccgt cgacccgact ccgtcgcgga tcgccgacga gctccttcct ccgccgcggc
     8821 cccgcggaag cgggtcttcc cgcgcccgac gctccgtcag tcctgatggc ccggcaggta
     8881 gagcgagcgc gccgtcagga cgcggtggtc gaggcccgcg gcccggtcga ggcgggcgac
     8941 gaggggcggg agcggcgccg aaaagcgcca gaggtcgccc cggccgccgt ccgcggcgaa
     9001 cgcctgctcc cactcgcccc accacggcac cggacgcgcg gcgatcggct ccacgacgag
     9061 gacacgcgct ccgcggcgcg cggcctcgag gagccgcggg aggagagtcc cgcgggaggg
     9121 gtccccgagc tcgttgacca cccatccgag gaggatcgcg gcgccgcggc cggggaggcg
     9181 ctcgcgcagg aggtcccccg ccgtcgcgcg gccgcgcagg gatagcgcgg cgtacgtccg
     9241 gcgcgcctcg ctcgccgccc agccgctcct ctccactccg aagacctcgg cgtccgcggc
     9301 gagcgcccag gccgcgcccg ccgcaccggt gccgcacccg aggtcgagga cgcgcgagag
     9361 gggcggctcg gcggcgccga cggaggcgac gatccgctcg acgacgagat ggtggagcgg
     9421 cccgtagaag agcgcgaagg cggcccgctt cccctctccc tcgaagaccg cccccgagcc
     9481 gatccggccc ctccgctcga cgtagagcga ggagaccgcc tggagcccct tgcgcacctg
     9541 ggcgaaggag agctcggcga gatggcgtcg ctcgaggtcc tcgagccagc ggtcgaacgg
     9601 aggttcggtc atgccgtcgg ttgccctccc ggcgtcaggg ctctccgagc tccttcgcga
     9661 cggcggcgac cgcggcctcg cccgcggcga gggccctcgc tgcggccgac gcggagagct
     9721 tcgccgcgtc ggcgggcgcc gggcgcgagc gcctccacgc ctcccgcgcc gcctcgacct
     9781 cggcccgcgt cgcctcctcg gcggcgagca ggccggggag cgagcgcgcc ttcagcgccg
     9841 cgagggcgcg gcggtccatg tagcgcttct tctcgccccg cgtgtcgggg aagaggcgct
     9901 tcctcagctc gagggctcgc gcggcgagag gcgccgggcc ccggcccgcg gccagcggcg
     9961 ccgggaccgc gccggcgtga gggaaaaccg gcacggcggc tcccgcgagc ggaaggctcg
    10021 ggacgatcca gagcgctccc gcctcgggtc cctctcccgg cgcgggcgcc tcgagaacga
    10081 tcgcggcgac ggtgtcgaga cggcagatgc tgtcgcccgt gtagacgagc ggcggtccgg
    10141 attcctcgcc cgtctggccg atcccggcgt tcccgacgtc gcgcgcggcg cgcaggatcg
    10201 tcgcgaggtc gagtccgccc gcagtcgacg acccgaggag ctcgatcgcg cgcgcctcgc
    10261 gcaggaagcc ggatcccgac cccttcgttc cgctgtggct gtagttcgag cggacgagga
    10321 ggccccgggc gccgtcgccc gatggggcgg cgtcgacgcg gacgtagcct ttcgccgacg
    10381 tctcgaagac cgcggcgccc cccttcgcgt cgatgacgcc gaagttcgcg gagacgtccc
    10441 gtccgccgga gttggagcgc gcgaggagcg cctcgaagtc gtccaccgtc gcgcacgact
    10501 ggagggcgag cttcatgaac gtcccctcgc ccgccgtgtc gccgctttcg aggttgtagg
    10561 aggccgagtt catcacgccg aagccggtgg agttgatccc ggcccagacc tggaggccgg
    10621 ccgcgtcccc gcggttcacg acgccgacgt agggatagcg tccgtcggcg aggtagacaa
    10681 cctggttgtc gctcccgtcc acgtcccggt ttttccagag catcgggcgg ccgctcctcg
    10741 aggccgacgg ggcgacgatc gcggtggtgc aggcgtccgc gggggagggc gcgaggggcg
    10801 cggccagtac ggcgaacgcg gcggcggcgg cgagccggaa gcgggtggcg tgaacggaca
    10861 cgaagtcctc ctcggccgtc catcgtagcc gaggccaggt cctcacggtc cgtgcgcacc
    10921 ccgcgcgggc cgcctccgct cggggcggtc cggcctccct cgaacggccg gcccgtggca
    10981 gagtgcgcgc atgcaggacc gtcctcgccg cgagagcttc ctcccctttt cccgtccgag
    11041 catcggcgag gaggagatcg ccggagtcgt cgaggcgctc cgctccggct ggatcacgac
    11101 gggcccgcgc accgcccgct tcgaggagga gctcgcggcc ttcaacgagg tccctcgctg
    11161 cctcgccctc ctgagcgcga cgacgggcct cgagctcgcc ctgggcgcgc tcgggatcgg
    11221 cgagggggac gaggtgatca cgacggcgca cacgttcgtc tgcaccgtct cgaacgtcgt
    11281 cctccgcgga gccgtccccg tcctcgtcga cgtcgaggcc gacacgatga acctcgacgc
    11341 ggagcggatc gaggagcgga tcactccgcg gacgaaggcg atcctcccgg tgcactacgg
    11401 cgggcttccg tgccgcatgg accggatctg ggccgtcgcg gagcgccatg gcctcgccgt
    11461 cgtcgaggac gccgcccata cgatgggggc gcgctacgcc ggccgccgga tcggcgccga
    11521 cccgcggtcg gcgttctccg tctactcctt ccacccgaac aagaacctga cgacgatcga
    11581 gggaggcgcg atcgcgttcc acgatctccg ccacgaggcg tacctgaagc tccagcgttt
    11641 ccacggcatc gcgcgcggcg cggggacccc ctaccccgag ggctccccgc tctacgacgt
    11701 cccgatgccg ggccggaagt cgaacttcac cgacgtccag gcggccgtcg gcctcgccca
    11761 gctcccgaag ctcgacgcgt tcgccgcgcg ccgccgcgag ctcgcgcgcc tctacctctc
    11821 actcctcgcg gaggtgccgg aggtggagct ccccgccgac ggcgacgccg agcgcgagca
    11881 ttgctggaac ctcttcgtcc tccggaaccg ccccgaggcg accggccttt cgcgcgacga
    11941 gctgacggcg gcgctgaagg cggagaacgt cggcacgggc atccactggc aggggctctt
    12001 cgcgttctcg ttctaccgcg actacttcgc gcggcacccg gaggggctcc cgaaggacgg
    12061 cctgccgcac gcgacgcgga cgacggagaa cctgatgtcg ctcccgctct tccccgcgat
    12121 gacggaggag gacgtcctcg acgtcgtctc ggcgctccgg cggatcgtcg ccgccggtcg
    12181 acggcgggcg cggagctgac acgggctttc ccgaagggac ggctcgaacg aatgagctcc
    12241 gggcccctcg cgcgcgtcgc cggcgaggcg tcagtcgggc tggggggctt cggcggccgg
    12301 caggcggcgg aggatccggt agaacgagga gcggctgatg ccgaggagcc gcgccgcctc
    12361 caggcggttc ccgcccgtcc tgcggagcgc ctcctccagt cgcgccgagg tcacgtcgac
    12421 gatcgggctc cggcctccgc gcggctcgct cgccgggagc agcatctggg agcggcccca
    12481 gagcgcccgg aagtccgtcg gttcgaggat cggtccgtcg ccgaagaccg ccgcctgctc
    12541 gacggcgtgc ttcagctccc ggacgttccc ggggaacgga tagcccgtca gcaggtcgag
    12601 ggcctccggc gagagcgtcc gctccggacc gccctgctcc cggagcttgc ggaggaagtg
    12661 ctcggccagg agcgggatgt cctcggggcg ctcccggagc ggcgggacga ggagctccag
    12721 cgcggagagg cggtagtaga ggtcctcgcg gaactcccgc cgcccgatcg ccgcctccat
    12781 gtcgaggctc gtggcggcca ggacgcggac cctcacgtcc gtgacgcggt tctcgcccac
    12841 ccggcggacg cgcccttcct cgaggacgcg cagcagcttc gcctgcgcgg agagagggag
    12901 cgccccgatc tcgtcgagga agagggtccc gcggtgcgcc tcctcgaaga gcccccggcg
    12961 cgcctcggtg gctccgctga acgagccgcg ggcgtgtccg aagagctcgc tctcgacgag
    13021 ggtgtcgggg agcgccgcgc aattgaccgc gacgaagggc gccccctcgc ggtttccgag
    13081 ggcgacgatc aggcgggcga cgacgtcctt ccccgatccc gtctccccgc ggatgagcac
    13141 ggtgacgccg agcggggcga ctcgggtcgc ccgctcgacg agccgacacc aggctgcgga
    13201 ccggccgacg gcgaacgccg ccgcttcacc ggggacgacc attcgggcga gtctagcctc
    13261 tcgacgggcg attcgcgcta tcgtccgccc gtgtcgaacg tccgcctcct gctcctcgag
    13321 cccgacccgg cgcggtcggc gcggatcgcg cggtcgttcg aggagctgcg gacgccggtc
    13381 accgtcgcgg cctccgtcga ggaggtcgcc ctcgcggccg cctcgggccc cgtcgtcgtc
    13441 gcggcgagcg ggctcggcgg cggcggagcc gcccgccttc gggccctccg cgaggacccg
    13501 cggaccgcct ccctcgccgt cctcgccgtc gccccccgcg gggcgggcag cgtcgccgcg
    13561 ctccgggccg gtgccgacga cgtcgtgccg tggccgtcgc cggcgcccgt cgtgagggcc
    13621 cgcctccggg gcctcttcgc cgacgcgcgc gcccgggccg aatcccaccg aacggtcgcc
    13681 gccttcgccg gcctcctcga ggcgttcgag gcgcgggagc cccaccggat cgagcactcc
    13741 gcccgcgtcg ggcggctcgc ggccgggatg ggccgccggg cgggcctgtc ggaagccgag
    13801 atcgagcggg tccgggtcgg cggagtcctc cacgacatcg ggacggtcgc gatccccgac
    13861 ggcatcctct acaagcgcga gcgcctcacc gacggcgagc tcgccgtcgt caagtcccac
    13921 ccggtcatcg ggttcagcct gatccgcgac gtccccgccc tggagccgta cggccccttc
    13981 gtcctccgcc accacgagag gatcgacggg tcgggctatc cggacggcct acggggcgcc
    14041 gagatcccgc tcccggtgca gctcgtcgcc ctcgccgacg cgttcgacgc gatgacctcc
    14101 tcgcgcccgt accgcgccgt ccgggacgac gggcagacgc tgagcgtcct cgaaggggag
    14161 gcccgccgcg gccagtggga ccgggctctc ttccctctcc tcgtcgccga ggccgaggcc
    14221 cagggaggct gacggccctt tttgacaagg ggcggccccc gttgggagac tccccgcccg
    14281 gacactgcct ctcgccaccc ggcgcggggc gggccccgta ccacgatcgg ccaggagtac
    14341 gccgtccccc gacgacgggg cgggccggcc aaggagaaac atgagcaccg tcctcgaccc
    14401 gaaggtcgcc cccgcgtcct ccgcccgacc gaagttcaag gtcgccgacc tctccctctc
    14461 cgagctcggg cggaaagaga tccgcctcgc cgagcaggag atgcccggcc tgatggcgct
    14521 ccgcgagcgc caccgcggcg agaagccgct cgccggcgag cgcgtcatgg gaagcctcca
    14581 catgaccgtc cagacggccg tcctgatcga gacgctcgtc gagctcggcg cggacgtccg
    14641 ctgggtctcc tgcaacatct tctccaccca ggaccacgcc gccgcggcgg tcgtcgtcgg
    14701 ccggcccgag agcggcggaa cgcccgagag cccgaagggg actcccgtct tcgcctggaa
    14761 gggggagacg ctcgaggaat actggtggtg cacccgcgag gcgatcgagt ggcccgacgg
    14821 gagcggcccg accctgatcg tcgacgacgg cggcgacgcg acgctcctcg tccacaaggg
    14881 gctcgagttc gagacggccg ggaaggtccc cgcgttcgac ccgaagaacg accccgagga
    14941 gtggggcgtc atcctcgagc tgatccggac ggagctcgcg aaggacggga aggtcttctc
    15001 ccgcctcgcg aaggcgatcc gcggcgtctc cgaggagacg acgacgggcg tccaccgcct
    15061 ctaccagatg gagaaggacg ggacgctcct cttccccgcg atcaacgtga acgactccgt
    15121 gacgaagtcg aagttcgaca acgtctacgg ctgccgccat tccgtcgtcg acggcctgaa
    15181 ccgcgcgacg gacgtcatgc tcggcggcaa ggtcgccgtc gtcctcggct tcggcgaggt
    15241 cggcaagggg tgcgccgagg cgctccgcgg gcagggctgc cgcgtcatcg tcaccgagat
    15301 cgacccgatc tgcgcgctcc aggccgcgat gcagggctac gaggtgaaga cgctcgagga
    15361 cgtcgtcgcg accgccgacg tcttcatcac cgcgaccgga aacctcgacg tcatcaccgc
    15421 cgagcacatg gcgaagatga aggacaaggc gatcgtcggg aacatcggcc acttcgacaa
    15481 cgagatcgac atggccggcc tgaagaaggt ccccggcgtc acgcggatca acatcaagcc
    15541 gcagtacgac gagttccgct tccccgacgg gcactccgtc atgatcctcg ccgaggggcg
    15601 cctcatgaac ctcggctgcg ccacgggcca cccgagcttc gtcatgtcct cgtccttcac
    15661 gaaccaggtc atggcgcaga tcgccctcgc gaagaaccgg ggggagtacg gcaagaaggt
    15721 ctacgtcctc ccgaagaagc tcgacgagga ggtcgcccgc ctccacctcg cgcacctcgg
    15781 cgtgaagctg accacgctga cgccgaagca ggccggctat ctcggcgtcc cggccgaggg
    15841 gccgtacaag cccgatcact accgctactg accgccgcga agcgcccgtg atcgaccgaa
    15901 ggcccggggc gacccgggcc ttcgccgttc cgtccccccg acgagggact ctccgtcacc
    15961 tcccggaggg cgcgaacgac attcgcccga gcgtcggcgg ctccgccggg ccgctccggg
    16021 aaaggcgccg gaggtgctcc gcgaggaggt cggcgtcgag cggctcgacg cccccgagcc
    16081 gccgcgcggc ggcgagctcg gcgtggccgt cccctccggc ggcgaggaag ctcgagaccg
    16141 cgacgcggta cgtcgcgtcg tcccggagcg gcgcgtccgc gacccgaact gcgaggagcc
    16201 gccggcccgc cggcgcgccg aggtcggcct cgagcgtcat cccggagagc tgcaggaggc
    16261 cgcccgtcct ccgcccgacc gccgcgaggc tcctctcgag caccgcccgg agctccgccc
    16321 ccgcgagctc caccgtcacc acgcggttcc cgaacggcag ggcggaaacg acgtgccgca
    16381 tcgagagggg cccggcgggg agaggagcgc ggaggctcga accgggaacg agggcgacgt
    16441 ccgcgcccgt ccccgcgcgg aaggcgtcgg ccacgaggct tccgagcggt gtctcccgct
    16501 cgtgactccc ggtctcgtcg agcggccggg cgagggctcc ggccggagcc gcgagctccc
    16561 tctccagccg cgccatgagc tccttctcca tctccgagag ctcggggtcg ggggcgacgg
    16621 agacgtcgac cgggtgcacc gcgacgcgcg aggagatccg gcctccgctc ctctcgagcg
    16681 tcagctcgac gaccgacccg aggttgccgc tcggcgcggc gaccggacga ccgtcaaacc
    16741 agcgaacgac cgagaccgtc tcggattcct cctcggtcag aacggcgtcg acctccggga
    16801 cctcggcgag cagccgccgg ttcacctcga acccggcctg cgtcaggacg acgatcgcct
    16861 cggccccttc ccgccgaagc gccgccgcct cgcgcgccac cgcctccacg aggtcggtct
    16921 gccggaccgc tcggtggggt gtcgtcgtgc cgaggtcgtc cgtgaggccg aggaagccga
    16981 cggtgagccc cccgacccgg acgagacggc gcgtcgggag cgccgcgaac ggacgcccgg
    17041 cctcgtcgac gaggttcgac gtgatccagg ggaaggcgga gcggcggacg aggcgctcgg
    17101 cgtgggccgg cccgaagtcg aactcgtgct ggccgaacgt cgcgaggtcg acgccgacgc
    17161 gaccgagggc gtcgaccatc ggctccccgc ggaagacgcc gccgaagagc gtgccgcccg
    17221 cgaggtcccc gccgaagacg aggagcgcgt ccggccctcc ggccccgagg cggtcgacga
    17281 ccgtcttcag gcgcgcgacc cctcccctct cgccgtgccg gtcgacgacc ggcgcgatct
    17341 cgtgagcgtc ggtgacgtag aggatcgtcg cgcgcggcgc ggtcgcgcag ccgagccaga
    17401 gcggcagcgc gaggaggagg agggccccgc cggctctcac gggaggcggg acccctcgac
    17461 gccgcgggcg ggcaccggcc tcagcccttc cggggccagt actccggctc gacgtcgtcc
    17521 cggacgaccg tcgtcccggc ctctccccgg agcatccggg ccgtgtcctc cagcgctccg
    17581 atgcaggccg cctttccggt cgctacggcg aactcgcacg cggcctggac cttcggcccc
    17641 atcgacccgg ccgggaagga gtacttcttc agatcggcga tcgagaccgt cttcagggcg
    17701 cgcgcctcgg gcgtccccca cccgacgtag acggcgtcgg cgtccgtcgc catgacgaac
    17761 gcgtccgcgc cgacctcgcg cgccaggagc gaggaggcga ggtccttgtc gatgacggcc
    17821 tcgatcccct gcagcttgcg gtcggggccg tacatggtcg ggatgcctcc gcctccggcg
    17881 aagatcacga cggtcccctt ctcgaggagc cacttcaccg gccggatctc gaagatccgc
    17941 ttcgggtgcg gcgaggggac gacccggcgc cacccgttcc cgtccgcctt gaaggtccag
    18001 cccttctcgg cggccatccg gtcggcgtcc tccttcgagt agaccgggcc gatcggcttc
    18061 gtggggttcc ggaaggccgg gtcggccggg tcgacctcgg tcatcgagag gatcgtggcg
    18121 aaggggcgct cgaacgggag gaggttcccc atctcctgct cgatgacgta gccgatcatc
    18181 ccttccgtct gggcgccgag gacgtcgagc ggatacggct ccacgtgggc ataggccgat
    18241 tgctggagcg ccaggagccc cacctgcgga ccgttcccgt gcgagatgac gatctcgttg
    18301 tcgttcgcga ccggcgcgag cgcctcgcag gccctcttca cgttggcccg ctggttctcc
    18361 gccgtcatcg gctccccgcg cttgaggagg gcgttccctc cgagggcgac gacgacgcgc
    18421 atgtctctac tccttgtctt ccatcttgcg gccccccccc ccgcggcgac gagaagccgg
    18481 gggcacgggg gccctgctgc tcatgttctc gggggacccg gacgccgggt cgacttcgtc
    18541 ttcgcttcgc ggtccccgaa cccctccgcg cgagcttgct ctcaggccag agtcgcgacg
    18601 aggacggcct tgatcgtgtg gagccggttc tcggcctggt cgaagaccac ggacgccggc
    18661 gactcgaaga cgtcgtccgt cgcttccatc gcctcgatcc cgaacttctt gtagatctcc
    18721 tcgccgatct tcgtgtcgcg gttgtggaac gagggaaggc agtggaggaa cttcgccttc
    18781 gggttccccg tcgccgccat gagcgccgcg ttcacctggt acgggaggag gagcttgatc
    18841 cgctcggccc agaccgagtc gggctcgccc atcgagaccc agacgtcggt gtggacgaag
    18901 tcgacgccct tgacggccgc cttcgggtct tcggtgagcg tgaccttccc gccggacgcc
    18961 ttcgcgagct cgttcgcctt cgcgacgagc tcctcgaccg gccagaggtg cttcggggcg
    19021 ccgaggcgca cgtccatccc catgagcgcg ccgccgagca tgagggtgtt ccccatgttg
    19081 ttccgcgcgt cgccgaggta ggcgtaggag acctgcccga gcggcctgtc gctgtgctcg
    19141 cgcatcgtga ggaggtcggc caggatctgg gtcgggtgga actcgtcggt caggccgttg
    19201 tagaccggga cgccggccca cttcgcgagc tcctcgacga tcgcctgccc gtagccgcgg
    19261 tactcgatgg cgtcgtacat ccgcccgagg acccgcgccg tgtccttcat cgtctccttc
    19321 ttcccgatct gggagccgga gggtccgagg taggtgacgt tcgccccctg gtcgtagcag
    19381 gcgacctcga aggcgcagcg ggtacgggtc gagtccttct cgaagatcaa cgcgacgttc
    19441 ttcccgccga ggcgcttcac ctcggtcccc gcgtacttgg cccgcttcag gtcgcgcgcc
    19501 aggtcgagga ggtagaggat ctccttcggg gtgaagtcgt ggatcgtcag gaagtggcgg
    19561 ttcttgaggt tgaacgacat gggtcctccg gcgacggctt tcccctggcg gcggcgggac
    19621 cgctccgggc acctcccgcg aggctccttc cggacgtccg tcgagcgcgc gccgcccctc
    19681 cccggggcga aagccggatt ctagaatgga agaccgtttg accgcgcccc ccgtccggaa
    19741 atacgcttcc gggttatgaa gcgggactat gcgatctccg gaacgaccct catgctcgcc
    19801 gcggccctcg gcggcgccct cctcacccag gcggcctgca agaagagcgg acccgacatc
    19861 aaggtcggtg tcatcgccga gctgagcggg gacatctcgg agatcggcga cgcctgcaag
    19921 aaggcggcgg agctggccgt caccgagatc aacgaggccg gcggcgtcga ggtcgccggc
    19981 acgaagcacc tccttcgcct cgtcatcgag gacagccagg gggccgccga gccggccgcc
    20041 gaggcggcga agcgcctcat cgaccaggag aaggtcctcg cgatcgtcgg accgaacgcg
    20101 agcttcggag cggtcccggc cgcccaggtg gccgcggaga agaagacgct cctgatctcg
    20161 ccctggggga ccgcgccccg cgtgacgctg gacgacaccg gtgcgccgcg gacgtgggtc
    20221 taccggacct gcttcaccga cgccttccag gggtcggcgc tcgcgaagtt cgcggcggcc
    20281 tacatgaagg cgacgaaggc cgcggtcctc tacaacccgg cgtccgaggc cccgaagagc
    20341 caggccgagc tgttgaagaa ggagttcgag gggcgcggcg gccaggtcgt cgccttcgag
    20401 acctacgcgg ccggcgacaa ggacttctcg gcccagtgga agaggatcgc cgaggcggct
    20461 cccgacgtcg tcttcctccc gagctattac ggggagatcc ccgagcagat ccggcaggcc
    20521 cgggcggcgg gcgtgaaggc ccccttcctc ggcagcgaca cctggacgaa ctccgagctc
    20581 ctcaagagcg ccgcggaggt ggacggcgcg ttcttctgcg cccactacaa ccccgacgcc
    20641 atggaggaca aggtcggcat cttccgcggg gcctacgaga accgttacga caaggaggtc
    20701 ccggacgacg gcgccgccct gacgtacgac acgctctacc tcctgaagca ggctctcgag
    20761 accgcgacca gcgacgaccg cgaggcggtg cgcgaggcct tctcgaagat ctcgagcttc
    20821 gacggcgtga cgggccggat cgtcttcaag ggaggcgccc ccgacccggt gaagagcgtc
    20881 gtgatcaagc agttcaaggc ggggaagatc tccttcgtga ccaatatcga ccccgactga
    20941 gatcgacccc ggccgaacga ggccgtcctc ggcctcgttc ggcccccacc cgggcctccg
    21001 gccctctccc gtatcgaccc cgacggaacg cgacagcttc acggcattcc tcaaagggcc
    21061 cgcacgcgcg agcctttcgt gctctcccgc gttccgcggg cgcccggcgc cgaatagccg
    21121 gcggcgtgcg aggatcgtcg ggacggacgc gccatgaagt acacccagct cggccgcacc
    21181 ggcctcgtcg tctcccgcct cgccctcggc tgcatgagct acggggaccc gcgctggcgc
    21241 ccgtgggtcc tttcggagga ggacgcgcgg cccttcttcc ggcgggccct cgaggcgggg
    21301 atcaacctct tcgacacggc cgacatgtac tcgctcggcg cgagcgagga gctgaccggg
    21361 cgcctcctgc gcgagatggg gcgccgcgac gagctcgtga tcgccacgaa ggtccacttc
    21421 ccgatgggcc ccggcccgaa catgtcgggg ctctcacgca agcacgtcgt ccaggcgtgc
    21481 gaggcgagcc tccgccgcct cggcgtcgag acgatcgacc tctaccagat ccaccgcttc
    21541 gaccccgcga cgccggtcgc cgagacgctc gaggcgctcg acctcctcgt ccggcagggg
    21601 aaggtccgct acgtcggggc gagctcgggc gaggccttcc ggctggcgaa ggcgctcgcg
    21661 accgctgacc tccgcggcta cccgcgcttc gtctcgatgc agaaccacta caacctcctc
    21721 taccgcgagg aggagcgcga gatgctcccg ctctgcgccg aggaggggct cggggtcctc
    21781 ccgtggtcgc cgctggcccg aggcctcctc gccgggacgc gggccacgcc cgcggaccgc
    21841 gaggcgacgc cccgtgccgc ctcggacgac tacgcccggc agctctacga ccacccctcc
    21901 gacgccgacg tcatcgacgc gacgcgttcg gtcgccgcgg cccggggcgt gccgatggcc
    21961 cgggtcgcgc tcgcctggct cctctcgcgg cccggcgtca ccgcgccgat cgtcggcgcg
    22021 acgaagctcg cccacctcga ggacgcgctc gccgcgctcg acctggcgct ctccccggag
    22081 gagaccgccg cgctcgagga gccctaccgg cctcacgccg tccggggctt cgccttttcg
    22141 ccgcccgccc gctgaggcgg acgcggccta tcatccggac cgtcccggag gaggtcccca
    22201 tgagagtctt cgtcaccgga gccaccggac acatcggctc ggccgtcgcg gccgcgttcg
    22261 cccgagcggg gcacgagacc tgcggcctcg tccgggaccc gcagaagacc gcgtccctcg
    22321 aggccgcgga ggtcgtgccg gtcctcggca cgatggagga gcccgacgcc tggagggagg
    22381 tcgccggacg ctgctccgtc ctcgtccatt gcgccgtcga ctcgtcggac cgctcgtggg
    22441 acctccaccg cgccgcgctc ggggccctgc gcgacgccgc tcgcgatcgc gtcgaccggg
    22501 tcctcctcgc cacgagcggc gtctgggtct acggagacac gggggaggcc gccgtcgacg
    22561 agtcgacccc gctcgatccg ccgaccttct ccgcgccccg tccggccgtc gacgccgccg
    22621 tcctctcctg gaacggcgcc gggctgcgga cgctcgtcat ccggcccgga tgcgtctacg
    22681 gcggcgcggg gagcctcacc gcggactggt tcgagagcgc ctcgcgggac ggcgccgcac
    22741 gcgtcgtcgg ggacggtacg aaccgctggg cgatggtcca ccgcgacgac ctcgccgacc
    22801 tctacgtccg ggccgccgag tcgggcctcg ccggcgaggt gctcaacgcc accgacagga
    22861 gccggtccac ggtgctggag tgcgccatcg ccgcgagccg cgccgcgggg gcggaggggc
    22921 gcgtcgagca cctgccggtc gagcaggccg tcgccgcgat gggcccgttc gcgaccggcc
    22981 tcgccctgaa ccagcacgtc gcgtcgtgga aggcggtccg gcggctcgga tggacgcctc
    23041 gccacgcggg tttcgtcgac gaggcccccc gcctcttcgc ggcctggcgg gcctcccgga
    23101 cggaggccgc gtgagcgccc ccgcgatcgg ctcgatcggc tggctcgacc tcacggtcga
    23161 ggacgccccg cgcctccgcg acttctacgc ggccgtcacc ggatggcggc cggagccggt
    23221 tccgatgggg gactactccg actgggcgat gatcgacccc tcgtcgggcc aggcgcgcgc
    23281 cggggtctgt cacgcgcgcg gcgggaacgc gggcctcccg gccgcctggc tcgtctacgt
    23341 cgtcgtggcc gacctcgacg cgagcgtcgc ggccgcgacg gagcggggag gggaagtcct
    23401 gctcgggccg aaggcgatgg gcgagagccg ctacgccgtc ttgcgcgacc cggccggggc
    23461 cgccttcgcc ctgtggcaga acggggcgtg accgcgaccg caccgccgtc gggacgcgcg
    23521 cgtcgcgccc ggcccggctt ccgtccggcc gtcgagccct gccggaagcg cgtatcccgc
    23581 cgccgcgatg cgagttcagg aatgggccgt acaatccgcg aaacggtcct gcattgagcg
    23641 tccttctcag gcctcccgag ttcgatcact actccgtctc ccgcgtcctg ggcgaggggg
    23701 ggatggggat cgtcttcctc gcggaggacc ggcggacgca ccttccggtc gccgtgaagg
    23761 tgatgtcgcg gagcctgacc gaccccgagc tccaggcgcg gttcatgaag gagaaccaga
    23821 tcctggcctc gctgaaccac cggaacatcg tccggtgcta cgagatcacc cgctcgcgcg
    23881 aggggctccc gaccatcgtc atggagtacg tgaaggcggt cgacttcggc gccttcgagg
    23941 ggcggccgtt cccggagctg atcccgctca tggtccaggc cgcgatgggg ctcgcgtacc
    24001 tgaaggaacg gaacatcctc caccgcgacc tctcgccgaa caacatcatg gtcaccctcc
    24061 aggacgagcg ccgcctcgtg aagatcctcg acttcggcgt cgcgaaggtc cttcaggagg
    24121 ggagcggcgg ggagctgaca cagaccggcg agttcctcgg gaagctcgcc tacgcctcgc
    24181 acgagctcct gacgttcggc caggtcgact tccggagcga catctactcc ctcggcgtca
    24241 tcttcttccg tctcctgacg aagcgccgtc cgatcaacgt ccagaactcg aggaactacc
    24301 tcgagtgggt catggctcag gagaaccgcg ggccgatcga cttcgccgcc cccgagggga
    24361 acccggcgat ccccgactcg ctgcaggccc tcgtcctgaa gatgctcgcg aagaagtccg
    24421 aggaccgacc gcaggggtac gaggagatca tcgacgccct cgtcgccgcg cagtccgagg
    24481 cggagaagtc cggcctcgtc ccggaccccg agacggtctc gactctcccg tctcccgagg
    24541 cgcgcagctc gatctccggc tccgccggga ccccggcttc gcccgccggc tcgtctccga
    24601 ctccgggcgg ctcgggtgcc gcgggactct tcgacgacgg cgcgttcccg ttcaccgaag
    24661 cagagacgaa ggccccgtcc tcctccccga gcctcgccgg ttcgcggctc cccgctccgg
    24721 cgccggcccc gaaggccacc cccgcctcgc agatgcccga ctggctcgag caggccgact
    24781 acaacgaaca gatgcaggtc atcagcgaga gcaagcaggg gatcccccgc tcgccggtcg
    24841 tccaccccgc gttcggcggc gagtcgcgcc cgcgtccggt caggcgggac accgagtcga
    24901 tccgtgtgcg ccagaaggaa gcgtcctccc ggaaacgcac gaccgtcctg gtgtcgctcc
    24961 tcgtcgtcgc gatcctcggc ggaggcgcct gggccggctg gaccttctac gtcaagccga
    25021 acctcccctc ctcctcgccc gaggcgctcg ccctcccggc gccacccgat ccggcctccg
    25081 tggcgccgcc gacggcgccc gagaccgtcc tcccgacggc ggtcccggag cccgcgaaga
    25141 ccgtgagccg ggccgcgaag ctcggttcgg ccgagaggcg cctcgtcgac gcgaacgtcg
    25201 ccggcttcta cgcctccgcc gacacgatgg ggagagggct cagcatcatc ctcgagttcc
    25261 ggcagcccgc gaacgtctcg ggcctgaagt cggcccggat cgtctcggcc accgacgcct
    25321 ccggacggaa cgtgccgggc gtcgccgggg cgacggccga gatccgcttg aacctcaacg
    25381 acgacccgac gagcctggtg cgcgtccgcc tcttcgccgt cggcgagaac gcggccgcgg
    25441 cggccgcctc cctccgctcc atctccgtcg agatcggctc ggcgacgatg cgcgccaggt
    25501 tcgacccacg ggtcctctcg aggtaggcgc ggtggcggcg tcccgtccgg acgcgcgctg
    25561 ccgtggcgat cgccgcgggc gtatttcgtg agacgttcct ccgccgtccc gccgacggtg
    25621 acgttctggg tagcccaggc aggcgacgtc cccaccctcc tcccgatgat gcgggcgctc
    25681 tgggagcacg aggggatccc gttcgacgag gggctcgtcg agccgccgct ccgcgagctc
    25741 ctctccgagc ccgcgctcgg tcgcgtctgg ctcgcgctcg cgggcggcgc cgtggccggc
    25801 tacgcgatgg ggacgtgggg gttcagcacg gagcaggggg gtcgcttcct cctcctcgac
    25861 gagctcttcg tccttcccgc cttccgcggg acgggaatcg ggcgggcggc gctcgcgttc
    25921 gtcgaggagg ccgcgcgcgg cgcagaaggg cgtgccgtcc ggatcgaggt ggccgaggag
    25981 aatgacggcg cgcggcggct ctaccgcgag gcgggctacg tcgacccgcg gcgcctcttc
    26041 ctcgcgaagc ggctgaccga acgctgacga cgcccgacgc cgggacgctt cggggcctac
    26101 actccacgcg gagacgcgac cgcgacgtgc cgctcagacg catcgagatc gagggcttcc
    26161 gtgggatccg gcggatgacg ctggacctgg acgacacgac cgcgctgatc ggagagaact
    26221 cctgcggcaa gacgagcgtc ctcgatgcgc tcgagctcgc gttcggtcac cgcggcgcgc
    26281 ggcccgcctt ctcacctctc gacttccacc tcccggtcgg gacggcggct ccggtcgagc
    26341 ggatctcgat gcggttcacg ttcgccgggg agggcgggcg cgagaccgtc gccgagctca
    26401 cggcctcacg gacgcccgac ggctcccccg ccgtcgccac gtcccggctc acggaccgcg
    26461 acggacgcga cctgagcccg ggagacgagg aggccgtcga ggcgttccgc cgccggcatc
    26521 cggtcctgcg cgtccgcttc aatcggcccc cgcgagtcgc gcccgtggag atcgacctgg
    26581 agaccccggc gaccggcccg gaggcgcccg cgccggccgt cgtcccgcca gcgcccgggg
    26641 aagccggcga gggcgaggtc acccggaagc tccgtcggac ggtcgagagc gcctaccgga
    26701 agttctcggg ctcctggcgg ccccatcccg aagagctccg ggagccgatc gcggccgccc
    26761 gcgctctcgc ggagcgctgg gtcgagcgcc ttcgcctccc gccccgcgcc ccgggcggac
    26821 gcacgaccga gatcctcgcg gagaccccgg tcctcctcgg cgccaccgat cctctccggg
    26881 ccgagtcgcg cgaggggacg ggcatgcaga gtctcgcgct cctgatgctc ctcggggccg
    26941 tcctcgacgc cgagaatggc tcccccgtcg aggcaggtgc cgagccgctc gtcctgatcg
    27001 aggaagcgga ggcccatctc cacccgatcc tggctgcgcg cgtcttcggc ctgatcgacc
    27061 gcgtcgccgg gcagaagatc ctgacgacga actcgggcga cctgctcgct tcggtccccc
    27121 tccacgcgat tcgccgcctc gtccggaacg ggggcggagt ggccgtccac ggcctcgccg
    27181 agggggcact gacggtcgac gagatgcggc gcgtcggcta ccacatccgc gtcaacaggg
    27241 cctcctcggt cttcgcccgc tgctggctcc tcgtcgaggg ggagaccgag tcgtggctcc
    27301 tcccggagat cgcccggctc atcgggcacg acctccccgc cgagggggtc cggtgcgtcg
    27361 agttcgctca gtgcggcatc gcgccgctcg tgaagatggc gcgcggcctc gggatcgagt
    27421 ggcacctcct cgccgacggg gacgacgccg gacggcacta cgtccgggcc gcccgctcct
    27481 tcctccaggg agagcccgac cgcgagcgtg tcaccggctt ccgcgagcgg gacgtcgagc
    27541 accacctctg gggggccggc tacgccggcg tctaccgggc cgccgcccag cgtgccggcg
    27601 tcttcccgtc gctcttcggc cgccggcgca tccccacgac cgcgatcatc gaccgcgcgc
    27661 tgaaggcgca cgggaagccg cgcatggcgc tcgaggtggc cgaggcgatg gccgcgcccg
    27721 attccccggg ggtgccgcgc ctcctcgcgt cggtcgtcga gaaggccgtc cgcctcgccc
    27781 gcttctccac ctgaccggtt gacggggcct cttccccgct agcccgcgag gagcgggatg
    27841 gcgtcggtga gccgcgccac gttcagcacc ggcgccggaa gcggacgacg ggaggggttg
    27901 tagaggaccg ggagccagcc cgctcccttc gcgccgacgg cgtcggtctc gtagtggtct
    27961 ccgaggtaga gcttccggcg gccgtccgct ccgtcgcctc cgcacgccga tcggaccgcg
    28021 gcgtcgaaga tctcacgtgc cggcttcatc gcgcccgcct cctcggagag gacgacgtgc
    28081 ttcacccagc gctccagccg gaaacgcgcc agcttcgccc gctgcagctc ccggaagccg
    28141 ttcgagagga tcccgacctc cccctccttc gcggcggccg cgaggagctc ctcggcccct
    28201 tcgttcaggc gccaggcgcc acggtagcgc tcgaggtaga acgcacccgt ctcgtccgcc
    28261 gcttccgggt cgcacccgag ctccccgaga ggatcggcga accgtcggcg gtgaagctcc
    28321 tcccgcccga tctccccgcg gccgtaggcc gcccagaggc gaccgttcgc ccggcgatac
    28381 gcggcgagcc attcgtcgaa cgccaccgcg ccgaagacgt cccgcctccc ctcgaacgtc
    28441 tccagctgcg cccgcgcctc cgcccccgcg tggtcgagca gcgtgtcgtc caggtcgaag
    28501 aagagcagcc ccctcacccc cccatcctcc cacactcccc ccactccggt gccaggcgtg
    28561 aaatcgtgta ttgttcttcc gggacgtccc ggcccgacgc gcccccgcgc cgtcggcttc
    28621 aggcaaccgt gcgatggtgg ggcgtcacgt cgtgccggcc ggcgtcactc cgcgagcacc
    28681 cgacgcagct ccgccagccg gcgcgcttcc gcggggtctt cggcgaggcg gcgctcgagg
    28741 aacgccacgt cgcgcgagat cgtcgaggcg tcgcgcccga agaagcgggc ggtcctcgcg
    28801 aacgggatcc cgccgtactc ccggcccaga agggcgcaga ggctccggac ccgggagagg
    28861 ctccggaggc gggacggccc cgtcagctcg tcgacggaga cctcctcgtg cgccgcgacg
    28921 gccttgacga cggcttcgac cgtgagcgtg cgcctcacga ggtcttcccg ccccgccccc
    28981 gcgagcgccg tccggacgaa gtcctcccct ccgacgaccg ccccgaaccg ggctccgtcg
    29041 gggtcgtacc gccccgccga cgaccccgtg acgaactcct ggaagagccg gcgggccttc
    29101 ggtcgtcccc cggcgaggag accgaggacc tccccggagg cgagccacga gggacccgtc
    29161 gagaggtagg cgccgtggct cgaccagggg aactcctcgg gccgcgcgac gaggcgtgcc
    29221 ttcaccgggt tgcggtggat gtaccggacg acctcgagga gatatccctc gcggtcgacg
    29281 atgattccct ggaaccgtcc ctggaagaga tggcccgatc gctcccagcg ccggttgaag
    29341 tactgcgcat accgcgagtg gacgtcgtgg acgaggcggg agatcggcgc gctccccgtc
    29401 tgcagagcga ggtggatgtg gttcggcatg aggcaccacg cgaacgccct cgcgccgtag
    29461 cgaccgcacg cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggcac
    29581 cggctccggg gagagttgag gtcagcggcc gaaccaggcc tgagtgtcgc ggcggaggaa
    29641 ggcgacgagg aggggaatgg cggcgggcat cgtgcagcag ctcgtcaggc cgaaggcgat
    29701 caggacgagg tgcgcgaccc acgcccaggg ccggcgcggg aggaagtagc cggcaacgta
    29761 gagggcgagg aaggggacgc cgaggccgag aagcaggccc ccggtcagga cgatctccca
    29821 cggctcgtcg cgcggaggcc agtcggcgac ctcctgccgg aagacgagaa ggagcgcgcc
    29881 gccgacgacg caggcgaggt agatcgccgc catcgcgccg aggtagacgc gctgccagcg
    29941 gaggcaggcg ggctcgacga cggggagggc gaaggcaggg ggcggagccg gccggggcgg
    30001 agggggcggc gggaggagcg gcggagcggg gggcggggcg cccacgtcag acgacgtcga
    30061 cgcgatcgag gaagccgacg acgacggagc ggacgggggc ggtcgaggag ccgacgacct
    30121 ggcgggcgcc gttcccttcg tcgaggacga ggaccgggtc gccggggccg acaccaatgg
    30181 cgtcgatggc gatgacgtag tcgcccgtct cggcgccgtc cgcacccgtc cgcgcgacga
    30241 cgagcatctt cttcccctcg aagaaggggt ggcgaatcgt cgccgtgatc tcgccgatca
    30301 cgacgccggt gatcacgccg gctccttcgc ggggaggtgg agcgcgtcga cgatgccgac
    30361 gacggcgtgg tcgaccggga cgaaccaggg gtcgagaagg agcgcggcct cgcggcctcc
    30421 ctcgtaggcg acgagctccc cttcccccgc catccgggtc ggatcggcgg cgacgagggt
    30481 cttccccttc ggcgcgccgt ccttgccgag cggctggagg aggagcatcg ggaccccctc
    30541 gagaccgggc gtcacgacgc acggcacgac gcggccgatg acgcgcccga ggagcactac
    30601 gaggcctcct ctccgccgag gtcgacgaag cggctggccg cgaacgcggt gccgagcgcg
    30661 gcctcgcgcg cgaaggcgtc gtgggggcgc gcaaggacgc ggtacgagag gcccgcggcg
    30721 ggcgggaggg ccgacgtcac gatctcgacc gccgcctcca gctcgtggag gtcgccgcgg
    30781 aagagggcga gccccttgcc cgcgaggccc gcgtccgaca ggcgcacctc gacgaggtcg
    30841 acgagcgttc ccttgagcgc ccgctcgcag gcgaggacga cggaggaggc cgtcgccgtc
    30901 tcgaggatca ggagcgcgcc cgtgccgtcc tcgccccggc gcccctcgag ggcgtcgagg
    30961 aggcggggat ggacgtcggc gaggtagagg tggtcgagga ggtgcccctc ggcgcgggcg
    31021 agcccctcgg cgagcgcctc ctcgacggag gcgggcgtcc cgccgatgag cgtcaggtag
    31081 cgcccggccg tcaccgtccc cgagcggaag aaggcgacgg gggccttctt cagcatcgcg
    31141 tccgtcgcgt cgagccccgc ggggatctgg gcgaagtcga ggacggcgag ggcggcggcg
    31201 cgcttcatg
//
LOCUS       DAOONL010000033        30306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1316_length_30306_cov_19.079237, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000033 DAOONL010000000
VERSION     DAOONL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 30306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_30306_cov_19.079237"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1804
                     /locus_tag="PLP50_07165"
     CDS             335..1804
                     /locus_tag="PLP50_07165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA51367.1"
                     /translation="MEGIEGARTTREDTDGRTLRRALVFALLVGGGARLFLALARPLW
                     ADEVFTLTLARRPLVDLLSALRLDSGPPLHYVAAKLLLLPFPAPGTADVVVRLLSVAA
                     SLLHVPLLLRIGRRRGAARAGLVGAALFLLFPLAASSGAEGRGYALASLLALATFERL
                     LSLAEAPRPRTALAAGLLGGAAVLTHYLALLPVAGLLLAALARREGRRLVVFAGSVAA
                     VVAAAWLPVALGQPHASMAWSEARPLSERALQSATNLGLGIPVDPGPSRYAGPLAAAV
                     LGLAIAGGRRARVPAATPLLLGALGLLPLLVYSRSAVLPDRTALVFLPLVALVLAQAR
                     WSEVALASGAASVVILALSVPHWVRPTPAAELAAALAPRVRDGARVVAAELWGPELDY
                     RLARDGLAGRVTFFPSDVALHPGWYSDAGPAEERLREESEAVVGGVAGGRALFVFSPA
                     TRAGLALRRRMEEARAERVAAAGPFEVWAAGFARDGGGR"
     gene            1801..2955
                     /locus_tag="PLP50_07170"
     CDS             1801..2955
                     /locus_tag="PLP50_07170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51368.1"
                     /translation="MRVLLVVPRLPGTGFTGDRVRAELHLEALAGLGARVTIVGGVPP
                     NRPVAAVAGTEEVRGVPVRVSALPLHLVRAAFRGWPLQTALFDGDWKRALDGTGPFDL
                     VVLLLARLHPRLQPLLPKAPLVVDYVDALSEAARQNAERDPALWRRLYWRAEQARLAR
                     AEREAARGARLLLATTPADAAALPEGTEAIAIGARIGPPPPRERAPVVVFSGRMGYRP
                     NAVAAELLLREIWPRVRERVPRAELVVGGADAPSSLRRRAAATPGARLLSPVSDMRAL
                     LVSARVAAVPVDMGTGTPNKVYEALEAGCAVVATPAAASRAVLDGVAAPVRTASGPEA
                     FAREVVELLTDAKGAEELGEAGRRFVVAHADRGEIALSLGRLLRRAAEEA"
     gene            2952..4292
                     /locus_tag="PLP50_07175"
     CDS             2952..4292
                     /locus_tag="PLP50_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPA51369.1"
                     /translation="MRHASPGRRALLAAGDVLAAVAAFAAVVLLRRLVPLPGTEGVLP
                     AGKVPLDPWPWLAVVGLAALLAHSLAATWSEPLETLRERGGLFVASVLTALFLVGAFF
                     LLSRALPRTVALLYVPVAWSLHALFRALVDRAAPPPVRRVLVLGDGEDARRAAAALEG
                     GEAPGYTLVDWVPDGAGAIALGRGPGAPHQVDDVVLASGNPREREAFASLVERSAAGG
                     FELWLLPGLADVVASHVATRSLGDLPLTRVAARGASAPAFALRRILDLVLGLPLALLA
                     AIPVAAVSLVVVLETPGPAWIRQRRVGRGGRELHVWKIRTMRADAEKETGPVLSRVGD
                     ERVTRVGRLLRKARLDELPQLLNVLGGSMSLIGPRPERPELVATFAAEEPLYRLRHLL
                     KPGLTGLAQVMGAYATKPDVKLRYDLGYLFQWNPVLDLFVLVRTVTTVLRVSGV"
     gene            complement(4279..6099)
                     /locus_tag="PLP50_07180"
     CDS             complement(4279..6099)
                     /locus_tag="PLP50_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPA51370.1"
                     /translation="MRNGPEDATAAGRELLAPLGTLVALSLPFFVLALGSAQATRDAK
                     LAAQAAGAVLVLLGLAYGSPSATREPAGRGARLALVAFPVFLALLAASATAGVLGGRD
                     PLDLLPVLPALALFAWGSTRPGEASAGRALALLVACGALTGLLAALQRFAGLFRLPVQ
                     APEPRFFATALIGNPGDVGAALVVPALLAAASLARGERRLASALAFAALLAGLAAAAT
                     LAPIAAFGAGAAVLVLADLRRRLLPALGAGLVLLGLLQAGGVLSRVSEKLAAGEVGAL
                     TTQRDIGVLAALETVRAHPVLGVGPGGFSADFVRARLAAEERAGRRLVHRSASAHFDN
                     AHNDPLTVAAEAGVPAAVALAFVLAALLVPLVAATRRERAGKRPGGLPAEALLASLAA
                     VLVLSLANFPIQIVPVSGPFALLAGLALARGGGSVPLPSGRAGRTALALLALLLATGA
                     AVRLAGGRALARAETALRLVPGASGEGRSALVASALADARRAVALRPRSATAHLALGT
                     ARAAGADPAGAVASMERSRELEERAETLLNLGRLAIARGDDHAARPFFLRAVWVFPRL
                     AAAIPPAGDPEGVVAEVARLEAALAAGGAPPPAPPPLRLR"
     gene            6143..6715
                     /locus_tag="PLP50_07185"
     CDS             6143..6715
                     /locus_tag="PLP50_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPA51371.1"
                     /translation="MPILKPEPDLCPDDLFALPEERFPWWVIHVKSRQEKLLARECRR
                     RGVPFYLPLCEQRSARDRRGRIAYVPLFTGYLFARGDLEHERLEILKTNLCLQVLAVP
                     DQEGLSADLAQVRRLQEMGMPLVPWPELTKGSAVRIAEGPFRGMTGRVTKLKGRSRFI
                     VSVRFIHRSVSVEMARDALVPAVLERPTRR"
     gene            complement(6636..7775)
                     /locus_tag="PLP50_07190"
     CDS             complement(6636..7775)
                     /locus_tag="PLP50_07190"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPA51372.1"
                     /translation="MRRSQGERPGPHPAALRAALLRWFRKHRRDLPFRRTRDPWAVWL
                     SETVLQQTTVAAGAPRFERLLARFPTVEALASADERELLAAWSGLGYYARARNLHRAA
                     RLVAERGGLVPRTVDELRALPGVGPYTAAAVASLAFGVPIPLVDGNVSRVLSRLGAIP
                     GDGRSGRSGAAVAEAAADFLDRRRPAEHNEALMELGALVCLPRAPRCPDCPLRAGCRA
                     LALGRPEELPAPRARKPAVRLRLAAGVARRDDRLVLVADGHLVRGHLVVPMLELPSEA
                     DAARLLRTAWPGLAGRRARRLAPLGRVRHSVLERLYAVDVFDVEEAGPTDESVPVRLV
                     LPRDLESEPRGGLLGKVLALSASSGVPAPPARARPAPSRPRRTGG"
     gene            complement(7783..8379)
                     /locus_tag="PLP50_07195"
     CDS             complement(7783..8379)
                     /locus_tag="PLP50_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA51373.1"
                     /translation="MERPPGTVAAVATAAGGPTDLELVERIRQGDQAALDLLYKRYSA
                     PVYSLVWKVLQSSEEAEDVALDVFWQVWRQAGRYDPSRGAPPAWIFTLARSRAIDRLR
                     ARKRREGRTVSIDDPDLNIDPLDEKAAPDEVVSFRQNRDAVRAAMAKLSATQREAVEL
                     AFLEGMTHVEIAERLGQPLGTVKTRIRQGLIRLRKHLD"
     gene            complement(8391..9050)
                     /locus_tag="PLP50_07200"
     CDS             complement(8391..9050)
                     /locus_tag="PLP50_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPA51374.1"
                     /translation="MTPPVLTVPLLASSARRSDAQQHLELHPLGGLRGEPPEADATRV
                     GLLGGIRHLHVFFEVRAEPPLRVRSRRLHGNVWEDECVELFWADAAEPSRYLEVVVNP
                     EGIHYTARIANPHASRETWHVVRGVPVPGLRIRVEGSPANEPATRWTSWRCGISVPWA
                     VLTPPLGRPRPGEERRGNLTRIARGRSLRFETLAPTGRATPPDFHVPSAFATFRFVEI
                     L"
     gene            complement(9047..10885)
                     /locus_tag="PLP50_07205"
     CDS             complement(9047..10885)
                     /locus_tag="PLP50_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPA51375.1"
                     /translation="MSRLRNVVCLAAALTPLLAAAATPAPPPDPPRRMVVVSFDGAGG
                     LDYARHRDALSPDGFRRAEREGVSAERLQTVTPSLTAVAHASISTGALPQATGIVSNT
                     YWPAGGPLKARVDGFAAEPAVETLWEALARQGHRVAALSWPGVSGASTRSSTPVGLKW
                     ASLRSPGFVWRGPEEGAVLPDAAIALPPEVRSYSPPRLVRVEPARGRDGAAPRSLLFV
                     VVDTRDDGRREYDGLAALDETGGLEGRLRPGEWLALSERSDGVALRGRWVKLLRLAPD
                     ASRVELYVGGIGQTEAWPDDFQRTLDRRCGFWPGPPDRALLSGEPDVKSFLEMASRFS
                     EFFTAAYEVAERRGDWDVLLGYQPLLDEVGHELTPPEPGAAGFDAAHAERAEAAMRET
                     WRIADRAAARYLRFLELGGDVLFLSDHGQRPVRRVVHPAEILRRQGWITTDDLAGRPV
                     VRADSPADVAVSGGTALVVLNRAGEMPGGVLSPEEGAGVLADIASHLRDLKDETGAPL
                     FETVALRGEAATLGLDHPNVGDLLLLAGKGTALRSGFPRLGAEAPVLLPADVAGQHGY
                     APDPALDGIFFHVGGGLDPARLPLVRAVDVAARVAARLGLSPPGAR"
     gene            complement(10897..11661)
                     /locus_tag="PLP50_07210"
     CDS             complement(10897..11661)
                     /locus_tag="PLP50_07210"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPA51376.1"
                     /translation="MRELRERARRGGDGSLVHDVRVALRRLEATGRLFRGFPEAKDGD
                     ALRIAARDLRRRLSSLRSEEVGRTLLAERAGDEGLLRTVFPDELPALRVDPAEIARLA
                     KGVTDWRRRLAAALGGPFAPRSKAGRELRRRAGRRLRRRLERLAALLPPTSRTLHRAR
                     IAAKRLRYALELLEALEPRAAPLLDRLRPFQDAAGEAHDLLELAVRLRTAGPAADDPR
                     LGHLGRELQEEARRAVARARRRGAALRTALRRLETR"
     gene            complement(11778..13523)
                     /locus_tag="PLP50_07215"
     CDS             complement(11778..13523)
                     /locus_tag="PLP50_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA51377.1"
                     /translation="MKERRRRHGYDTVALPKPGPGEPSLEALAFSGLPDPAAVLDGTG
                     RLLAANDAARAEGAPAPEGASAPLGQLLPFWNDPESRDALLEATAAPGGARDFEVRIA
                     FEDGSRRRVFRVSARRLVGEAPARLVAVARDVTAVEAEKNAAPGPRSLEGTPASTDPV
                     TGFVTRTAFQELLEIAIEESARAKRSLALLFFDLDDFKALNDTHGLAAGDEYLRRLGE
                     ALLADLQPGRVFARLGGDEFGLIAPETNAAEAALEADGLVRFLTEFSPTFEGRRLQLT
                     ASVGVALHPAHGQRASDLMLAADLAMHQAKARGRARFILHDPNARERERIGLLHGQAD
                     RIRTALAGARFVPVYQPIAEVATGRIVAAETLVRLREDDGTLVRPDQFLDAAERFGFV
                     TAIDRVVIAGVFDALVASRKRISPDLEVSVNLSGLDFEDDALVAEISRLARLKGIRPS
                     RITFEITETAALRDLGRVQDFTGALTSEGFRFALDDFGVGFSSFRYLRDLPMSTLKFD
                     QSYVKDLPHKMENRVFVRGIAEICRGFGVKTVAEGVETPEILEILRELGVDRAQGYHV
                     GHPSLELPGRSGEAG"
     gene            complement(13520..14032)
                     /locus_tag="PLP50_07220"
     CDS             complement(13520..14032)
                     /locus_tag="PLP50_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51378.1"
                     /translation="MNGTEISIRPARREDVPVVARLIRDLADYERLAHECFADEAALE
                     THVFGPRPYVEVLLAEVDGAPAGFALFFHSYSTFLTKPGLYLEDLFVVPERRGLGLGR
                     RLLAELARLAVERGCGRLEWSVLKWNAPAIGFYERLGAVPMAEWQVYRLTAEPLRALA
                     GKAARAAGDA"
     gene            complement(14042..14680)
                     /locus_tag="PLP50_07225"
     CDS             complement(14042..14680)
                     /locus_tag="PLP50_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPA51379.1"
                     /translation="MNEPVAPFPFAVLAFSSLFSVVNPISGAPVFVSLTAHLRHAERQ
                     RAAVRAVLTGLATLAVFSAAGGAIFSFFGITVPAFQITGGILFALMSLRALEHGHEEV
                     PETSAPGRDPSIVPVGIPLIAGPGAISTVMVLVGQAGGASHRLALAGALVATLSVTLA
                     ILLAAPAITRRLGETGMKITNKVMALITAVIGIQFVINGTTAVAVQILRAAR"
     gene            14748..15359
                     /locus_tag="PLP50_07230"
     CDS             14748..15359
                     /locus_tag="PLP50_07230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51380.1"
                     /translation="MTRTDENPAGGRRLAPYFGLLTRRVQRARLAAALPHVRAGARLL
                     DLGCGLTDLPARFPSYVGCDRNPVVLDEMRRLHPSASFASWDVEADEPPPAVREGAPY
                     DAILMLAILEHLAEPARALARAGALLAPAGRLLATTPHPLGRLPLEAGAHLGLLSRHA
                     DEEHETLLDRAGLERCATEAGLGVVSYSRFLLGLNQLVVLARR"
     gene            15356..18052
                     /gene="uvrA"
                     /locus_tag="PLP50_07235"
                     /pseudo
     CDS             15356..18052
                     /gene="uvrA"
                     /locus_tag="PLP50_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            18154..19335
                     /locus_tag="PLP50_07240"
     CDS             18154..19335
                     /locus_tag="PLP50_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA51381.1"
                     /translation="MSEGSHRYVLVVDGREIELAAGEVTLGRSRSSTVRVDHESVSRS
                     HALLTLSNGKAVLKDLNSSNGTFVAGRRILNETILRPGDRLQLGAAVLEYRLLEPTGA
                     LGRTAHLTTTAAEALSAVAGAQGDLGARSDAAPEPSASAALEISAHELFREADEKAQK
                     GGLAPVGAAAALAAVHGSGVRAEPPPVPLPPPEAPKGAPAPPVPEATLSQFPIRSDGR
                     PSAVRPAPAPKVRRPRSSTSLSTAAEAAGLFPRLLATLVDGVILLALDLLLLSPVFLV
                     FLFKDAFQSTDLLRDWAFLAIAGGCAVLVLGANLWYVVGGWARTGRTPGKALMRLSIV
                     LVASGRAAPGIGLGPAFGRFLAWNLSGAVLGVGHLVALFRKDRRTMHDLLAGTRVVRG
                     R"
     gene            complement(19346..20254)
                     /locus_tag="PLP50_07245"
     CDS             complement(19346..20254)
                     /locus_tag="PLP50_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51382.1"
                     /translation="MKCRSARSTSRDRLIRVALGAALSLAAFAPRAGLPAPQQSILPR
                     DAAARPFLEGRELVEKGRFREAIPKLESALSTGHQTPQLRFGASRHQVDYYDPHYWLG
                     RALMELGEEERALSHLRASAAGGSFPDRRETEDRRLRIVELERREAARRAPPPRTPTP
                     TPPPAPTPLPPAPTPAPTVSAALETPELVPTPPAEPPATPLLLVPTPVPPRLEGPLAA
                     LAAGDFETTVERVRAERKRTPDARELDLVEAAALGGRFVLEGRRDRSLLDRARESLAA
                     FRSKGGSARAEATLLSPALRTLLETR"
     gene            20308..23436
                     /locus_tag="PLP50_07250"
     CDS             20308..23436
                     /locus_tag="PLP50_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51383.1"
                     /translation="MPRNLGSARVALTAGLLLLASGALAGPVPCPPDRPVLSGPSEVH
                     RLEGYWITWTNVLSPRGSGQPDTFLIERSEDPTFQTGVERLQPTTRTTATWGPPGGAP
                     RTIYHRVAVQSFCAGTSPVRVDSAVLAVHVTDQCRPTIGLPPPLASPALPPANTTYLV
                     SWDTLGAGEPGPGGGAQGLRFRLRRTAGGDVTESLNDAGAAAFTDPPGEYLYQVRTEN
                     ICGDASPWSEPLRVVVGTTKTSALFLVSAPRPVLLPVDARGEVRVAFVVRNAGSEPLD
                     VTATGGGAGLTAEPPTFRLESGRDLRVEARLPKAPSAETPFHGSVVLAADGDVSLRVP
                     IDVATRGLDTGGVPSGEPDTSRPAGWDEDAVDVDRNGNDVTRLLVNPGGVNAYLATSV
                     SEPWLVVSSADGKAWDRPLAPGESRPIWLRVDRSRRRAAIGTEIATVTVVTAGHEGEP
                     KSLSVIDDGPQVTGVGSPSANDDIPPLYLMKSRLLFPSLPNALDARGAGRFTSDLWLS
                     NVDALNAIQVVLTLTPAGQSNTSAGVRQFAFSLGAGETRRFRNVIGTIFGYEGGCSLD
                     VSSPSPTLSATALVNNKPVVPLVAGKTAALDSAIAGTVLSTAEFGFEMRPVAPGEGAS
                     QKDPDHVVSGLWHDARRRTNLILRETTGNETTVRVELFDGEGRAVLRDGIPVDFERTV
                     PGHGSVQINDDDLFPAGEFSLAFRAKVTFLRGSIDPFGQTHGAVVPLATVIDAGTQDA
                     ALRVGVSEKALTPVAVGSAPAARALATASALPFEGGPEPLLFPASHVTGAALSSGAPP
                     RWRSRVTFSNVSATEQRNVRLRFRPASGALPAGMLEQTSVVVPPGWGITFDDVLEQLF
                     GLPAGAEAWGTIEADNLERSDGSWLFTWSDVDVQTETYTADTEDPERGEFRTGMEGYS
                     YQHGYSSFQSNLGTVLIDGAEISARNRTNLILQEVGGASCKLAVGVYRPGALVPLAVR
                     IIDLKASEYLSRELFRDLMQLDLDEIVDARVVVRQLDGDGVFMAFVSKINLVTGDPAN
                     VFLRPASAGTGR"
     gene            23534..24892
                     /locus_tag="PLP50_07255"
     CDS             23534..24892
                     /locus_tag="PLP50_07255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA51384.1"
                     /translation="MVRRVGRGGMGIVYRAVDERDGKTVALKVIRETEVAGDESAPHV
                     AEARVRFTREAEILQGLLHANVVTFHEIGEEDGTPYLAMEFLEGRPIGSYAGHPYTET
                     LPLLIQAANGMEYLASRGIVHRDLSPDNVFVVESGSEKTVKLLDFGIAKLFESATSES
                     LTATGFFLGKVAYGSPEQLGSLGQGAPLDWRSDVYSLGVIFYQVLAGRRPFEGRAPVE
                     YIAAHLNLSPPPVAAPPDAPALPMELVRLIGQMLEKRREARPQSYREITERLVAILRS
                     SGAGVAGLLPEGDGAAPPGGPAAARAATSPTQLVSEKPRSPAVPARRVVGVLAAGAAL
                     LGLLLWGALRSGPREAPPARPADPVHLPPATAELRITSIPWGVVVSVVDVATGKAVAL
                     PASADTPLPLAVPPGRYRVTVVEPAPGTGRAETDAEAATGSPALVALTLRPLEEIVET
                     LR"
     gene            24889..26322
                     /locus_tag="PLP50_07260"
     CDS             24889..26322
                     /locus_tag="PLP50_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51385.1"
                     /translation="MKKPVLALAALLLVRIPAPAQVESALLSKVTFNLTNPGGKSLAM
                     GGAFTAIADDATAGIANPAGLGLLSSFEAGVSVKRVDDVIGLVTARSTATGGLVEPYP
                     DPRAANSDISGRSSLVEFAGIVIPVSRRVVAAVSFAENLSFEGDPGPDGYTYIELRDN
                     RSGGSTRNDYLFEFREHGAVSLRNRVLALSAAYRMTDRVRLGAGLTVNRMELNLLGDE
                     TGAHRIVHRTFTTPTVIETRTTTMAIRDLDRSVLGFVAGLHVDLVAGGKLTAGAVYRQ
                     TAKANGTLEIGGDVPVPLAGQQSRPFSARVPRDAAVGLAAQPIPGLTVAAEAQWIAYG
                     DFIDRSLPTVSYAGLVGPLPGMFVRDLLAEMTPPDDVVVPRLGIEYVATSGSLLLAFR
                     LGYHREPARGVTADLAAIEETTGMPFDVDEPPFSGSVRTVYDGGRPDDRFSGGLGATI
                     SRKVSFDLAFDLGRSSRVLSASLFYRF"
     gene            complement(26369..27328)
                     /locus_tag="PLP50_07265"
     CDS             complement(26369..27328)
                     /locus_tag="PLP50_07265"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51386.1"
                     /translation="MSRGLGEILAESGDLTFRRLRRASEWRRVHGGTIDRALLETGAV
                     REETLLDALCRLTGLPSVSRERLAAASREAVEALPADTRRRLRALPFDRRGDVLHVAV
                     VDPGNPVLETGLVASTGCDVRLYVTAEPILEDLLARWEEPGPAAPEESDLARPAAEQV
                     VAPAAGDRPRAATAEPSTKTARSAETDPFGRLARALLVDALDEGAEAVEIGAEGRGAV
                     VRSYAGGAVLSSRPIPAPVLDPLFAWLVERSRPAGPFDEGGLLLERAHRRVRVQVSST
                     GDGTAWLVLKAEATPPPAPAGNEICLHEAADGDVFCPACGAPV"
     gene            complement(27404..28012)
                     /locus_tag="PLP50_07270"
     CDS             complement(27404..28012)
                     /locus_tag="PLP50_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPA51387.1"
                     /translation="MSRPLTILMDEHRIIEQALGSLETCAIEVEGGLALERAVVRDYA
                     GFLKGFADAWHHGKEEDILFRRMVERGFSAEAGPVAVMLHEHRQGRELVGRIAAVGAG
                     EGPVAAEERASFLAASRGFVPLLRQHIQKEDNILYPMSTRVLTPEEFEKMLADFEAFE
                     RAAGAVGTRDRLSAVAEKLVAAFRPDEARMAAGAVGSCGSRG"
     gene            complement(28086..28493)
                     /locus_tag="PLP50_07275"
     CDS             complement(28086..28493)
                     /locus_tag="PLP50_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPA51388.1"
                     /translation="MFSRTSLHALRTLGYLAAHRERWVLAREIAEETGIPPNYLSKVL
                     AQLRKRGFVVSQKGWGGGFRMKGTGAGTTISAVLETFDGRREKDACAFGLGRCDSDDP
                     CPLHPHWEKVREEYERMLRTVTLRDLGGSGSAA"
     gene            complement(28576..>30306)
                     /locus_tag="PLP50_07280"
     CDS             complement(28576..>30306)
                     /locus_tag="PLP50_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPA51389.1"
                     /translation="GDLLEIEAEADPRLEIPEIHRRVIAQGGPALLFRRPKGSSFPVV
                     TNLFGTAKRIDLAFGKRPLDFVKTAVRASHELLPPTAGKLWGFRSFFREALRVGTREV
                     SAAPVTEVVETEPDLTRLPALTQWPEDGGPFLTLPLVYTEHPEKREHNLGMYRIQIHE
                     ARQTGIHWQIGKGGGFHHHVAEAKGEALPVTVFLGGPPALIIAAIAPLPEGLPELLLA
                     SLLLGKRLPLARVPGHPHRLVAGAEFAITGKVPPGVRRLEGPFGDHYGYYSLAHDYPV
                     LDVERVYRRRDAIFPATVVGKPVQEDFLIGDFLQELLSPLFPVVMPSVVSLKTYGDTG
                     FHSLAAAVVKDRYGREAISTAFRILGEGQLALTKFLLLTDQRVDLDDFRALLETVLSR
                     CRFETDLFVVSNVAMDTLDYTGPKVNEGSKGVLAGLGEPIRDLPRAFEGTLPAGAGAA
                     AVFCGGALCVSGPSWREDRDYAARLAADPAVAAWPLVALVDDAETAARDARSFLWHVF
                     TRFEPGADVHAARTEVVRNQVARTAPIVLDARMKSWMPAVVTPDPATVALVDRRWREY
                     FPARGAGARL"
BASE COUNT         3820 a        11075 c        11461 g         3950 t
ORIGIN      
        1 agggggagac gagacctgga cctgcatgga gcctcctcag gcggggcgcg ccggccgcac
       61 gcggacctcg cgggcggcgg atcgccgttg ggatgtgggg ccacgaaaaa gcccggccgt
      121 agccaggcgg acggagtcgt gctgagcgcg gcgaatcgaa atcaaggcgg gtctgccccg
      181 acccgaaatc gtagtcctga ggtcacctga agtcgaggaa atggtgtctc taaacagggc
      241 agaatcggtc cggttgttct gtaaccggac cgatcgggtc ccgatgaccc gtcgtctctt
      301 gcattcacct ccgggcggcc cgaaagtcgg cggagtggag ggaatcgagg gcgcgaggac
      361 gacgagagag gacacggacg ggcgaacgct ccgccgggcg ctcgtcttcg ccctcctcgt
      421 cggcggggga gcgcgactct tcctggcgct cgcccgtcca ctctgggccg acgaggtctt
      481 caccctgacg ctggcgcgcc ggccgctcgt cgacctcctc tccgcgctcc ggctcgactc
      541 gggcccgccg ctccactacg tggcggcgaa gctgctcctc ctgccgtttc cggcgccggg
      601 gacggccgac gtcgtcgttc gcctcctgtc cgtcgccgcg tccctgctgc acgtgcctct
      661 cctgctgcgg atcggccggc gccgcggggc cgcccgcgcc gggctcgtcg gggcggcgct
      721 cttcctcctc ttccctctcg ccgcgtcttc cggcgcggag gggcgcggat acgccctcgc
      781 ctctctcctc gcgctcgcga cgttcgagcg gctcctctcc ctggcggagg ctccgcggcc
      841 gcgcacggcg ctcgccgccg gcctcctcgg gggagccgcg gtcctgacgc actacctggc
      901 cctcctgccg gtcgcgggac tgctgctcgc ggccctcgcg cgaagagagg ggcgccgcct
      961 cgtcgtcttc gccgggtcgg tcgctgccgt ggtcgccgcg gcctggcttc cggtcgcgct
     1021 gggtcagccg cacgcgtcga tggcctggag cgaggcccgt ccgctttcgg agcgggctct
     1081 ccagtcggcc acgaacctcg ggctgggtat cccggtcgac ccgggcccgt cgcgctacgc
     1141 gggaccgctc gccgccgccg tcctcggtct cgcgatcgcc ggcggccggc gtgcgcgggt
     1201 tccggcggcg acgcccctgc tcctcggggc gctcgggctc ctgccgctcc tcgtctacag
     1261 ccggtcggct gtcctgcccg accggaccgc gctcgtcttt ctccctctgg tcgcgctcgt
     1321 gctggcccag gcgcgctgga gcgaggtggc gctcgcctcg ggcgcggcgt cggtcgtcat
     1381 cctcgcgctc tcggttcccc attgggtccg gccgacgccg gccgcggagc tcgccgccgc
     1441 gctcgcgccg agggtccgcg acggggctcg ggtcgtcgcc gcggaactgt gggggccgga
     1501 gctcgactac cggctcgcgc gggacgggct cgcgggccgg gtgacgttct tcccgtcgga
     1561 cgtggcgctc catcccggct ggtactcgga cgcggggccc gccgaggagc gcctgcggga
     1621 ggagtcggag gcggtcgtcg gcggcgtggc cggaggtcgg gcgctcttcg tcttctcccc
     1681 ggcgacccgg gcgggtctcg ccctgcgccg ccggatggag gaggcgcggg cggagcgggt
     1741 cgcggcggcc ggtccgttcg aggtctgggc ggccggcttc gcccgcgacg ggggcggacg
     1801 gtgagggtcc tcctcgtcgt gccccgcctc ccggggacgg gcttcacggg tgaccgcgtg
     1861 agggcggagc tgcacctcga ggcgctcgcc gggctcggcg cgcgcgtgac gatcgtcggc
     1921 ggcgtgccac cgaaccgtcc cgtcgccgcc gtcgccggga cggaggaggt gcggggcgtc
     1981 ccggtccggg tgtcggccct tcctctccac ctcgtccgcg ccgctttccg aggctggccg
     2041 ctccagaccg cgctcttcga cggggactgg aagcgagccc tcgacgggac ggggccgttc
     2101 gatctcgtcg tcctcctgct ggcgcgcctc cacccgcgcc tgcagcctct cctcccgaag
     2161 gcccctctcg tcgtcgacta cgtcgacgcg ctctcggagg cggcccgcca gaacgccgag
     2221 cgggacccgg cgctctggcg gcgtctctac tggagggcgg agcaggcgcg tctcgcgcgc
     2281 gccgaacggg aggcggcgcg gggcgcgcgg ctcctcctcg cgacgacgcc ggcggacgcg
     2341 gcggcgctcc ccgaggggac ggaggcgatc gcgatcggcg cccgaatcgg gccgcccccg
     2401 ccgagggaac gcgccccggt cgtcgtcttc agcggcagga tgggttaccg tccgaacgcc
     2461 gtggccgccg agctcctgct gcgcgagatc tggccgaggg tgcgcgagcg cgtcccgcgg
     2521 gcggagctcg tcgtcggcgg cgccgacgcc ccctcgtcgc tccgccgaag ggcggccgcg
     2581 acgccgggcg cccggctcct gagcccggtc tccgacatga gggcgctgct cgtctcggcg
     2641 cgggtcgcgg ccgttcccgt cgacatgggg accggaacgc cgaacaaggt ctacgaggcg
     2701 ctcgaggcgg gttgcgcggt cgtcgccacg cccgccgccg cctctcgtgc ggtcctcgac
     2761 ggcgtcgccg ctccggtccg gaccgcttcg gggccggagg ccttcgcgcg cgaggtcgtg
     2821 gagctcctca cggacgcgaa gggggccgag gagctgggag aggcgggacg ccggttcgtc
     2881 gtcgcgcacg ccgatcgcgg agagatcgcg ctttccctcg ggcggctcct gcgtcgggcc
     2941 gcggaggaag cgtgaggcac gcgagccccg gccggcgcgc cctgctcgcc gccggggacg
     3001 tcctggccgc ggtcgccgcc ttcgcggcgg tcgtcctcct tcgccggctc gtcccgctgc
     3061 cggggacgga aggggtcctt ccggcgggga aggtcccgct cgacccgtgg ccgtggctcg
     3121 ccgtcgtcgg gctcgccgcc ctgctggcgc attcgctcgc cgcgacctgg tccgagccgc
     3181 tcgagacgct ccgggagagg ggcggcctct tcgtcgcgtc ggtcctgacg gcgctcttcc
     3241 tcgtcggcgc gttcttcctc ctctcccggg cgctgccccg gacggtcgcg ctcctctacg
     3301 tccccgtcgc ctggagcctc cacgcgctct tccgggccct ggtggaccgc gcggcgcctc
     3361 cgcctgtccg ccgggtcctc gtcctcggtg acggagagga cgcgcggcgc gccgccgcgg
     3421 cgctcgaggg gggcgaggcg ccggggtaca ccctcgtcga ctgggtcccc gacggggcgg
     3481 gggcgatcgc cctcggccgg gggccgggcg cgccgcacca ggtcgacgac gtcgtcctcg
     3541 cctcgggcaa cccgcgcgag cgcgaggcgt tcgcctctct cgtggagcgg agcgcggcgg
     3601 gaggcttcga gctgtggctc cttccgggcc tcgcggacgt cgtcgcctcg cacgtggcga
     3661 cgcggagcct cggcgacctg ccgttgacgc gggtcgccgc gcggggcgcg tcggctcccg
     3721 cgttcgccct ccggaggatc ctggacctcg tcctgggatt gccgctcgcg ctgctcgccg
     3781 cgattccggt ggcggccgtc tccctcgtcg tcgtcctcga gacgccgggc ccggcctgga
     3841 tccgccagcg ccgcgtcgga cgcggcggcc gggagctcca cgtctggaag atccggacga
     3901 tgcgggcgga cgccgagaag gagacgggcc cggtcctctc ccgggtcggc gacgagcgcg
     3961 tgacgcgcgt cggccggctc cttcgcaagg cccggctgga cgagctgccc cagctcctga
     4021 acgtcctggg cggctcgatg agcctgatcg ggccgcgccc cgagcggccc gagctcgtcg
     4081 cgacgttcgc ggcggaggag ccgctctacc gcctccgcca cctgctgaag ccgggcctga
     4141 ccggcctcgc gcaggtgatg ggcgcctacg ccacgaagcc cgacgtgaag ctccgctacg
     4201 acctcggcta cctgttccag tggaatccgg tcctcgacct gttcgtcctc gtccggacgg
     4261 tgacgacggt cctccgggtc agcggagtct gagcggcggc ggtgccggtg gcggcgcccc
     4321 gccggcggcg agcgcggcct cgaggcgcgc gacctcggcg acgacgcctt cggggtcgcc
     4381 ggccgggggg atcgcggcgg ccaggcgcgg gaagacccac accgcccgca ggaagaaggg
     4441 gcgggccgcg tggtcgtctc cgcgggcgat cgcgagacgg ccgaggttca ggagcgtctc
     4501 ggcgcgctct tccagctcgc gcgaacgctc catcgacgcg acggcgccgg cagggtcggc
     4561 ccccgcggcg cgcgccgtgc cgagagccag gtgggcggtg gccgacctcg gacggagcgc
     4621 gacggcccgg cgggcgtcgg cgagggccga ggcgacgagc gcgctgcgcc cctcccccga
     4681 ggcgcccggg acgagccgga gcgcggtctc ggcgcgcgcg agcgcgcggc cgccggcgag
     4741 gcggactgcg gcgccggtgg cgagaaggag cgccaggagc gcgagggccg tgcgcccggc
     4801 gcgcccggag gggagcggga cagagccccc gccgcgcgcg agcgcgagcc cggcgagaag
     4861 agcgaacgga ccggacaccg ggacgatctg gatcgggaag ttcgccagcg agaggacgag
     4921 gaccgccgcg agcgaagcga ggagcgcctc ggcgggaagg cctccgggcc tcttcccggc
     4981 gcgctctcgc cgcgtcgccg ccacgagggg cacgaggagc gccgcgagga cgaacgcgag
     5041 cgcgacggcc gccgggacgc cggcctccgc ggcgaccgtc agcgggtcgt tgtgagcgtt
     5101 gtcgaagtgg gccgaggcgg agcggtggac gaggcgccgg ccggctctct cctcggcggc
     5161 gaggcgtgcc cggacgaaat cggccgagaa gccgccggga ccgacgccga ggacggggtg
     5221 agcgcggacg gtctcgagcg ccgcgaggac gccgatgtcc cgctgcgtcg tgagcgctcc
     5281 gacctcgccg gcggcgagct tctccgagac gcgcgacagg acgccgcccg cctggaggag
     5341 cccgagcagg acgagcccgg cgccgagcgc gggtaggagg cggcggcgca ggtcggcgag
     5401 caccaggacc gccgctcccg cgccgaaggc ggcgatcgga gcgagtgtgg cggccgccgc
     5461 gaggccggcg agcagagcgg cgaaggcgag ggccgaggcg agccgccgct ccccccgggc
     5521 gagcgaggcg gccgcgagga gcgccgggac gacgagcgcg gcgccgacgt ctcccgggtt
     5581 gccgatgagc gccgtcgcga agaagcgcgg ctcgggggcc tggaccggca gccggaagag
     5641 gcctgcgaag cgctggaggg ccgcgaggag cccggtgagg gcgccgcacg cgacgaggag
     5701 agcgagtgct cgcccggcgc tcgcctcgcc cggccgggtc gagccccagg cgaagagggc
     5761 gagcgcgggg aggacgggga ggaggtcgag agggtcgcgc ccgccgagga cgccggccgt
     5821 cgccgaggcg gcgaggagag ccaggaagac ggggaacgcc acgagggcga ggcgcgctcc
     5881 ccggccggcc ggctcgcggg tcgcggaagg gctcccgtac gcaagcccca ggaggacgag
     5941 gacggccccg gccgcctggg cggcgagctt ggcgtcgcgg gtcgcctggg ccgaaccgag
     6001 agccaggacg aagaacggaa gcgaaagcgc cacgagcgtg ccgagcggtg ccagaagctc
     6061 ccggcccgcc gccgtcgcgt cttccggccc gttcctcatc ccgcgaagaa tagccggggc
     6121 ggtcgattgg taaagtgagg gtgtgccgat cctgaagccg gagcccgacc tctgtcccga
     6181 cgacctcttc gcgctcccgg aggagcggtt cccctggtgg gtcatccacg tgaagtcgcg
     6241 tcaggagaag ctcctcgccc gcgagtgccg gcgacggggc gtgccgttct acctgccgct
     6301 ctgcgagcag cggagcgcgc gagaccgccg cgggcggatc gcctacgtgc cgctcttcac
     6361 cgggtacctc ttcgcccggg gggacctcga gcacgagcgg ctcgagatcc tgaagaccaa
     6421 cctctgcctc caggtgctcg ccgtgcccga ccaagagggg ctgtcggccg acctcgcgca
     6481 ggtccggcgc cttcaggaga tgggcatgcc gctcgtcccc tggccggagc tgacgaaggg
     6541 ctcggccgtc cggatcgccg aaggtccgtt ccgcgggatg acgggccgcg tgacgaagct
     6601 gaaggggaga agccgcttca tcgtctcggt ccgcttcatc caccggtccg tctcggtcga
     6661 gatggcgcgg gacgcgctcg tgccggcggt gctggaacgc ccgacgaggc gctgagggcg
     6721 aggaccttcc cgaggaggcc gccccggggc tcggactcga gatcgcgggg gaggacgagc
     6781 cgaaccggga cgctctcgtc cgtcggtccg gcttcctcga cgtcgaagac gtcgacggcg
     6841 tagagccgct cgaggaccga gtgcctcacg cggccgagcg gcgcgagccg tcgcgcacga
     6901 cggccggcga gtccgggcca ggcggtgcgg aggaggcggg ccgcgtcggc ctccgagggg
     6961 agctcgagca tcgggacgac gaggtgtccg cggacgaggt gcccgtcggc gacgaggacg
     7021 agccggtcgt cccggcgtgc gacgccggcc gcgaggcgaa gccggacggc gggcttgcgg
     7081 gcgcgcggcg cggggagctc ctccggacgc ccgagcgcga gggcccggca gccggcccgg
     7141 agcggacagt cggggcagcg cggagcgcgc gggaggcaga cgagggcgcc cagctccatc
     7201 agcgcctcgt tgtgctcggc ggggcgccgg cggtcgagga agtccgcggc cgcttcggcg
     7261 acggcggctc cggagcgtcc cgaccggccg tcgccgggaa tcgctccgag cctcgagagg
     7321 acgcgggaga cgttgccgtc gacgaggggg attgggacgc cgaaggcgag cgacgcgacg
     7381 gccgcagccg tgtagggccc gacgccggga agcgcgcgga gctcgtccac ggtgcgcggg
     7441 acgaggcctc cgcgctcggc gacgaggcgc gcggcgcggt ggaggttgcg cgcgcgggcg
     7501 tagtagccga ggccgctcca ggcggcgagg agctcccgct cgtcggcgga ggcgagcgcc
     7561 tcgacggtcg ggaagcgggc gaggagccgc tcgaatcgcg gggccccggc ggcgacggtc
     7621 gtctgctgca ggaccgtctc cgacagccag accgcccacg ggtcgcgcgt cctgcggaac
     7681 ggcaggtcgc ggcggtgctt ccggaaccag cgaaggaggg ccgcacgaag ggccgcggga
     7741 tgcggcccgg gccgctcgcc ctgcgatcgg cgcatcggct cctcagtcga ggtgcttgcg
     7801 gaggcggatg aggccctgcc ggatacgggt cttgacggtg ccgagcggct gcccgagcct
     7861 ctcggcgatc tcgacgtggg tcatcccctc gaggaacgcg agctcgaccg cctcgcgctg
     7921 ggtggcggag agcttcgcca tcgcggcccg gaccgcgtcg cggttctgcc ggaaggagac
     7981 cacctcgtcg ggcgcggcct tctcgtcgag cgggtcgatg ttcaggtcgg ggtcgtcgat
     8041 cgagacggtg cggccttcgc gccgtttccg ggcgcggaga cggtcgatgg cgcgggagcg
     8101 cgcgagggtg aagatccagg ccggaggggc cccgcgcgag ggatcgtagc gaccagcctg
     8161 gcgccagacc tgccagaaga cgtcgagggc gacgtcctct gcttcctcgg agctctggag
     8221 gaccttccag acgagggagt agaccggcgc ggagtagcgc ttgtagagga ggtcgagggc
     8281 ggcctggtcc ccttgccgga tgcgctcgac gagctcgagg tccgtgggac ctccggccgc
     8341 cgtcgccacg gccgcgaccg taccgggagg cctttccatc gcgcccgagg ttacaggatt
     8401 tcgacgaatc gaaacgtggc gaaggccgac ggcacgtgga agtcgggcgg cgtggcgcgc
     8461 cccgtcggcg cgagcgtctc gaaacgaagg ctccgtccgc gggcgatccg cgtgaggttc
     8521 ccgcgccgct cttctccggg ccgcggccgt ccgaggggag gcgtcaggac cgcccaggga
     8581 accgatatgc cgcaccgcca gctcgtccag cgggtcgcgg gctcgtttgc cggcgacccc
     8641 tcgacgcgga tgcgcagccc cggcaccggc acgccgcgga cgacgtgcca cgtctcgcgg
     8701 ctcgcatgag gattcgcgat gcgggcggtg tagtggatcc cttccgggtt cacgacgacc
     8761 tcgaggtagc gggagggctc ggcggcgtcg gcccagaaga gctcgacgca ctcgtcctcc
     8821 cagacgttcc cgtgaagcct ccggctgcgg acccggagcg gcggctcggc gcggacctcg
     8881 aagaagacgt ggaggtgccg gattcccccg aggaggccga cgcgcgtcgc gtccgcctcc
     8941 ggcggctcgc cgcggaggcc gccgagcggg tggagctcga ggtgctgctg cgcgtcggac
     9001 cgtcgggcgg agctcgccag gagcggaacg gtgaggacgg gcggcgtcac ctcgcccccg
     9061 gcggagaaag accgagacgc gcggcgacgc gcgcggcgac gtcgacggcg cggacgagcg
     9121 gcagccgtgc ggggtcgagc ccgccgccga cgtggaagaa gatgccgtcg agggcggggt
     9181 ccggagcgta gccgtgctgg ccggcgacgt cggccgggag aaggacgggc gcctcggccc
     9241 cgagcctcgg gaagccgctc cggagcgcgg tccccttccc ggccaggagg aggaggtctc
     9301 cgacgttcgg gtggtcgagc ccgagcgtcg ccgcctcgcc gcgcagcgcg acggtctcga
     9361 agagcggtgc ccccgtctcg tccttcaggt cgcgcaggtg gctcgcgatg tcggcgagga
     9421 cccccgcgcc ttcctccggc gacaggacgc ctcccggcat ctcccccgcg cggttgagga
     9481 cgacgagcgc ggttccgccg gagacggcga cgtcggcggg cgagtcggcg cgaacgaccg
     9541 gccgtccggc gaggtcgtcg gtcgtgatcc acccctgtcg gcggaggatc tcggccggat
     9601 ggacgacgcg ccggaccggc cgctgaccgt ggtccgagag gaagaggacg tcgccgccga
     9661 gctccaggaa cctcaggtag cgcgccgcgg cgcgatcggc gatccgccat gtctccctca
     9721 tcgcggcttc ggcccgctcg gcgtgggccg cgtcgaatcc ggcggctccc ggctccggcg
     9781 gcgtcagctc gtggccgacc tcgtcgagga gcggctggta gccgaggagg acgtcccagt
     9841 caccgcgccg ctcggcgact tcgtaggccg cggtgaagaa ctccgagaag cggctcgcca
     9901 tctcgaggaa ggacttcacg tcgggctctc ccgagaggag agccctgtcc ggagggcccg
     9961 gccagaaccc gcagcgccga tcgagggtcc gctggaagtc gtctggccac gcctcggtct
    10021 ggccgatccc gcccacgtag agctcgacgc gcgaggcgtc gggcgcgagg cggaggagct
    10081 tcacccaccg cccgcgaagg gccacgccgt cggacctctc cgagagcgcg agccattcac
    10141 ccggtcggag tcggccctcc aggccgccgg tctcgtcgag ggcggcgagg ccgtcgtact
    10201 cccgtcgccc gtcgtcccgc gtgtccacga cgacgaagag gagggatcgc ggggcggcgc
    10261 cgtcgcggcc ccgggccggc tcgactcgga cgaggcgggg cggcgagtag ctccggacct
    10321 cgggcggaag ggcgatcgcc gcgtcgggca ggaccgctcc ctcctccggc ccgcgccaga
    10381 cgaagccggg cgagcgaaga gaggcccact tcaggccgac cggagtcgac gagcgcgtcg
    10441 acgcgccgga gaccccgggc caggagaggg cggccacgcg gtgcccctgg cgcgcgagcg
    10501 cctcccagag cgtctcgacc gccggctccg ccgcgaaccc gtcgacgcgg gccttcaggg
    10561 ggccgcccgc gggccagtag gtgttcgaga cgatgccggt cgcctgcggg agagcgccgg
    10621 tcgagatcga ggcgtgggcg accgcggtga gggacggcgt gacggtctgc agccgctcgg
    10681 cggagacgcc ctcgcgctcg gccctgcgga acccgtcggg ggagagcgcg tcccgatgcc
    10741 gcgcgtagtc gaggccgccg gctccgtcga aggagacgac gaccatccgc ctcggagggt
    10801 cgggcggcgg ggcgggcgtc gcggcggcgg cgagcagcgg agtgagggcg gcggcgaggc
    10861 agacgacgtt tcggaggcgg gacattcgcg tcaagcctac ctcgtctcga gcctccggag
    10921 tgccgtccga agggcggccc ctcgccggcg cgcccgggcg acggcgcgtc gggcctcctc
    10981 ctggagctcc cggccgagat ggccgagacg ggggtcgtcc gccgccgggc cggcggtccg
    11041 gagccggacg gcgagctcga gcaggtcgtg cgcctcgccg gccgcgtcct ggaagggccg
    11101 gagacggtcg agaagcggtg cggcccgcgg ctcgagagcc tcgagcagct ccagcgcgta
    11161 gcggaggcgc ttggccgcga tccgcgcgcg gtggagcgtg cgggaggtgg gagggaggag
    11221 cgcggcgagg cgctcgagac gccggcggag acgccgcccg gcgcgcctcc gcagctccct
    11281 cccggctttc gagcgcgggg cgaagggtcc cccgagggcc gcggcgagcc gccgccgcca
    11341 gtcggtgacg cccttcgcca gccgcgcgat ctccgccggg tcgacgcgga gggcggggag
    11401 ctcgtccggg aagaccgtcc gaaggaggcc ctcgtccccg gcccgctccg cgaggagcgt
    11461 gcgaccgacc tcctcggagc ggagcgacga gaggcgccgg cgaaggtccc gcgccgcgat
    11521 ccggagcgcg tcgccgtcct tcgcttccgg gaagccgcga aagaggcggc cggtcgcctc
    11581 gagtcgcctc agggcgaccc ggacgtcgtg gacgagcgac ccgtcgcccc cgcgccgggc
    11641 ccgctcccgg agctcgcgca ggttcgcgct ccgcgcctcg agcgcgtcct ccaggaccgg
    11701 gaggatcgtg cgggatcgac cgttcgccag ggcgggcctc cgccccccat cctaggcccg
    11761 aggtgtgaag tctcccctca gcccgcctcg cccgaccggc cgggcagctc gagcgacggg
    11821 tggccgacgt ggtagccctg tgcgcggtcg acgccgagct cacggaggat ctcgaggatc
    11881 tccggcgtct cgaccccctc cgcgaccgtc ttcactccga agccgcggca gatctcggcg
    11941 atgccgcgga cgaagacccg attctccatc ttgtgcggca ggtccttcac gtagctctgg
    12001 tcgaacttga gggtcgacat cggcaggtcg cgaaggtatc ggaacgagct gaagccgacg
    12061 ccgaagtcgt cgagcgcgaa ccggaagcct tcggaggtca gcgcgcccgt gaagtcctgc
    12121 acccgaccga ggtcgcggag ggccgccgtc tcggtgatct cgaacgtgat ccgcgagggg
    12181 cggatcccct tcaggcgcgc gaggcgcgag atctccgcga cgagggcgtc gtcctcgaag
    12241 tcgaggcccg acaggttcac ggagacctcg aggtcgggcg agatccgctt gcgcgacgcg
    12301 acgagggcgt cgaagacgcc cgcgatgacg acgcggtcga tcgcggtgac gaagccgaag
    12361 cgctccgccg cgtcgaggaa ctggtcgggc cgcacgagcg tgccgtcgtc ctcccggagg
    12421 cggacgagcg tctccgccgc gacgatgcgg ccggtggcga cctcggcgat cggctggtag
    12481 accggtacga atcgggcgcc ggcgagcgcg gtgcggatcc ggtcggcctg gccgtggagg
    12541 aggccgatcc gctcccgctc ccgtgcgttc gggtcgtgga ggatgaagcg ggcccggccc
    12601 cgggccttcg cctggtgcat cgcgaggtcg gccgcgagca tcaggtcgga ggcccgctgg
    12661 ccgtgcgccg ggtgaagcgc gacgccgacg ctcgccgtca gctgcagccg cctcccctcg
    12721 aacgtcggag agaactccgt caggaacctc acgaggccgt ccgcctcgag ggcggcctcg
    12781 gccgcgttcg tctcgggggc gatcagaccg aactcgtcgc cgccgagccg ggcgaagacc
    12841 cgccccggct ggaggtcggc gaggagggcc tcgcccaggc gccggaggta ctcgtcgccc
    12901 gccgcgagac cgtgcgtgtc gttcagcgcc ttgaagtcgt cgaggtcgaa gaagaggagc
    12961 gcgagcgagc gtttcgcccg ggcggactcc tcgatcgcga tctcgaggag ctcctggaac
    13021 gccgtccggg tcacgaagcc ggtcacgggg tcggtcgacg ccggcgttcc ctcgagagaa
    13081 cgaggtcccg gagcggcgtt cttctcggcc tcgaccgctg tgacgtctcg ggcgacggcg
    13141 acgaggcgcg ccggcgcctc gccgacgagg cgacgcgccg agacccggaa gacgcgccgg
    13201 cgggagccgt cctcgaacgc gatccggacc tcgaagtcgc gtgctccccc gggtgcggcc
    13261 gtcgcctcga ggagggcgtc gcgggactcg gggtcgttcc agaaggggag gagctgtccg
    13321 agcggggcgc tcgcgccctc cggcgcgggc gccccctccg cccgggcggc gtcgttcgcg
    13381 gcgaggagac ggcccgtgcc gtcgaggacg gccgcggggt cgggaaggcc ggagaacgcg
    13441 agcgcctcga gcgagggctc gccgggcccc ggcttcggaa gggcgacggt gtcgtagccg
    13501 tgccggcggc gccgctcctt cacgcgtcgc cggccgcgcg ggccgccttt ccggcgagcg
    13561 cgcgaagggg ctccgccgtg agccggtaga cctgccactc cgccatcggc acggcgccga
    13621 ggcgctcgta gaagccgatg gccggggcgt tccacttcag gacgctccac tcgagccggc
    13681 cgcagccccg ctcgacggcg agccgcgcca gctccgcgag gagccgccgg ccgagcccga
    13741 ggccgcgccg ttccgggacg acgaagaggt cctccaggta gagacccggc ttcgtcagga
    13801 acgtcgagta gctgtggaag aagagcgcga agccggcggg cgcgccgtcg acctccgcga
    13861 ggaggacctc gacgtagggc cgcgggccga agacgtgcgt ctccagggcc gcctcgtcgg
    13921 cgaagcactc gtgcgcgagg cgttcgtagt cggcgaggtc gcggatgagc cgggcgacga
    13981 cggggacgtc ctcgcgccgg gcggggcgaa tcgagatctc ggtcccgttc acagggccgg
    14041 attatcgcgc ggcgcgcagg atctggacgg ccaccgccgt cgtgccgttg atgacgaact
    14101 ggatcccgat gacggcggtg atcagcgcca tcaccttgtt cgtgatcttc atccccgtct
    14161 cgccgaggcg gcgggtgatc gccggcgcgg cgaggaggat cgcgagcgtc accgagagcg
    14221 tcgcgacgag cgcgcccgcg agggccagcc ggtggctcgc gccgcccgcc tgccccacga
    14281 ggaccatcac ggtcgagatc gcgccgggcc cggcgatgag cgggattccg accgggacga
    14341 tcgacgggtc gcggcccggc gccgaggtct ccgggacctc ttcatggccg tgctccaggg
    14401 cccggagcga catgagcgcg aagaggatgc cgccggtgat ctggaaggcc gggacggtga
    14461 tcccgaagaa cgagaagatc gccccgcccg cggcgctgaa gaccgccagc gtggcgagcc
    14521 ccgtcaggac cgcgcggacg gccgctcgct gcctctcggc atggcgcagg tgcgccgtca
    14581 gcgacacgaa gaccggcgcg cccgagatgg ggttcacgac ggagaagagg gacgagaacg
    14641 cgaggaccgc gaaggggaac ggggcgacgg gctcgttcat cgtaccggcc gggccggacg
    14701 gctatcatac ggggcgcccc ggggcgaccg tcgcgggacg ctctccgatg acacggaccg
    14761 acgagaatcc cgcgggcggc cggcggctcg ccccctactt cggcctcctg acgaggcgcg
    14821 tgcagcgcgc gcggctcgcg gcggccctcc cgcacgtccg cgccggggcc aggctgctcg
    14881 acctcggctg cgggctgacc gatctgccgg cccgcttccc gtcgtacgtg ggctgtgacc
    14941 ggaaccccgt cgtcctcgac gagatgcgcc gcctccatcc ttcggcttcc ttcgcctcct
    15001 gggacgtcga ggccgacgag ccgccgcccg cggtgcgcga gggcgcgccg tacgacgcga
    15061 tcctgatgct cgcgatcctc gagcacctcg cggagcccgc gcgcgccctg gcccgggccg
    15121 gcgccctcct cgccccggcg ggacgactcc tcgcgacgac gcctcacccc ctggggcggc
    15181 tgccgctcga ggcgggggcg cacctcggcc tcctctcccg gcacgccgac gaggagcacg
    15241 agacgctcct cgaccgggcc ggtctcgagc gctgcgccac ggaggcgggc ctcggagtcg
    15301 tctcgtactc ccgcttcctc ctcggcctga accagctcgt cgtcctggcc cgccgatgag
    15361 cccgcgaggg gcgcgcgccg tcgaggtggc cggcgcgcgg acgcacaacc tgaagggggt
    15421 ctcctgccgc gtcccgatcg gggcgatcac ggtcgtgacg gggccgtcgg gctccggcaa
    15481 gtcgtccctc gccttcgaca cgctctacgc ggaggggcaa cgccgcttcg tcgagtcgat
    15541 gtcgacctac gcccggcagt tcctggagcg gatggagcgc cccgacgtcg agcggatcga
    15601 ccatctcctc ccggcgatcg ccctcgagca gagggcgccc gcgcggtcgg cccgctccac
    15661 ggtcgggacc gcgacggaga tccacgacgt cctccggctc ctcttcgccg ccgcggggac
    15721 ggtcgtctgc ccggcctgcg ggctcgacgt gaagcgcgac tctccggcga gcgtggcgga
    15781 tgcgctcgtc gccggctttg gcgagggggc gcggctcctc gtcctcgccc cgcgcccggc
    15841 cgccgagctc gccgatcgcg cgccgctctg ggtccgctcc ggcctcttcc gcgccgtcgc
    15901 ctccgacggg agcgtcgtct cgttcgatgc cgggacgccg ccgcccgcgg gagcggacgg
    15961 cctcgtccgt ctcgtcgtcg gccggcacgt cctccgcgcg ggggacgccg agctcgccgc
    16021 cacgctcgac ggggccttcg cggtcggcga ggggacgctc ctcgcgcgcg acgcggcgac
    16081 ggggcgcgaa ctccgcttcc tgcgcggcct cgcctgcaac ggctgcggcc gggcgttcga
    16141 ggagccgacg ccggcccact tctcgttcaa ctcgccgcgc ggcgcctgcc gcgcctgcga
    16201 ggggttcggg cggatcgccg gcctcgacgc ggagaaggtc ctgccggacc cgtcgaagac
    16261 gctcctcgag cgccccttcg ccccgttcaa cacgcccgcc tacgcgagcg cctaccccga
    16321 cctctggcgc gcctgcaagc ggctcgggat ccggaaggac gtcccgtggc agcggatgac
    16381 gtcgcgggag cgggacctcg tctggaacgg cgacggcgac tggtacggcg tgaaggggct
    16441 cttcgactgg ctcgagacga agcgctacaa ggtgcaggtc cgggtcatga tcgcccgcta
    16501 ccgcggctac gagcgctgcc cggagtgcca cggggcgcgg ctgacgcccg aggcgcgcgc
    16561 ggtccgcgtc ggcggcgtga cgctccccgc gctttgcgac ctgccgctcg acgccctcct
    16621 cgacaggctc cgctccctcc cgctcgaccc gcacgagcgg gcccggggcg gggcgctcgt
    16681 cgacgacctc gtgaaacgcg tcgggacgct cgtcgacgtc gggctctcgt acctgacgct
    16741 ctcgcggacg acgcggaccc tctcgggggg cgaggcgcag cgggtccagc tcgcggcggc
    16801 gctcgggaac acgctcaccg gcaccctcta cgtcctcgac gagccgacgg tcggtctcca
    16861 ccccgccgac accgcgcggc tcctcgccgt cctccgccgt ctcgcggacg cgggcaacgc
    16921 cgtctgcgtc gtggagcacg acctcgacgt catccgcgcc gcggaccacg tcgtcgacct
    16981 cggaccgcgc gccggcgcgc acggcgggag cctcgtcttc gaggggacgc cgcgggcgct
    17041 cgcacgcgcg tcgaccgcga cgggagcggc gctgcgggcg gcgcacgccg cggtcgtcgc
    17101 cgagcggccg gcgccgtacc cgcgcggggc gatccgggtc gtcggggcgc gagcgaacaa
    17161 cctcgccgac ctcaccgtcg agatcccgac cggggtcctc gcgggggtct gcgggccgtc
    17221 cggttccggc aagtcgtccc tcgtcgtcga cgtcctcgct tccggggccc tccgcgcgct
    17281 cggccggaag gaggcggacg ccctcgagcg gggggcgcac gagaggctcg aggggctcgc
    17341 ggcgttccac gacgtcatcc tcgtcgacca gtcgccgctc ggccggtcgg cccgctccaa
    17401 cccggcgacc tacacgaagg cgtgggacga ggtgcgggcg ctctgggccc ggagccccgc
    17461 ggcgcgcgcg gcggggctga cgaaggggag cttcagcttc aacgcgccgg gcggccgctg
    17521 cgagcggtgc gaggggtcgg gcgtcgtcac ggtcgacatg cagttcctcg ccgacgtgac
    17581 ggtcgtctgc gacgcgtgcg acgggcgccg cttcacgccc gaggtgctcg gcgtgagggt
    17641 ccgcgggaag gacgtccacg agctcctcgg gacgacggtc gacgaggcga tcgagctctt
    17701 cgccgacctg ccgggaatcg cgtcccggct cgccccgctc tccgaggcgg gcctcggcta
    17761 tctgcgcctc ggccagtcga ccgcgaccct ctcggggggc gaggcgcaac ggctgaaggt
    17821 cgcctcgttc ctgaaggctg gagccgggaa gccgaccctc ttcctcttcg acgagccgac
    17881 gacggggctc cacgcgggag acgtcgacgt cctgctcggc gtctttcggc gcctcctcgc
    17941 cgcggggcac tccgtcctcg cggtggagca caaccccgcg ttcctcctcg cgtgcgactg
    18001 gctcctcgag ctgggcccgg gcggcggcgc cgaaggggga cgggtcgtcc acgccgggag
    18061 cgtcccggag ctcctctcgg gcgagacgac ggcgacggcc gtggcgctcc gggccgcctc
    18121 gggcgctccc gctcgcctac aatcggcgcc gtcgtgtccg aagggagtca ccgctacgtc
    18181 ctcgtcgtcg acggccggga gatcgagctc gcggccggtg aggtcacgct cggccggagc
    18241 cgctcctcga ccgtccgcgt cgaccacgag tccgtctcga ggagccacgc gctcctcacg
    18301 ctctcgaacg ggaaggcggt cctcaaggac ctgaactcct cgaacgggac gttcgtcgcg
    18361 ggccggagga tcctgaacga gacgatcctc cggccgggcg accggctcca gctcggcgcc
    18421 gccgtcctgg agtatcggct cctcgagccg accggggccc tcgggagaac ggcgcacctg
    18481 acgacgacgg ccgccgaggc gctctcggcc gtcgccgggg cgcaaggcga cctcggggcg
    18541 cgctccgacg ccgctccgga gccgtccgcg agcgccgcgc tcgagatctc ggcccacgag
    18601 ctctttcggg aggcggacga gaaggcccaa aaagggggcc tcgccccggt cggggcggcg
    18661 gccgcgctcg cggccgtcca cggctcggga gtccgcgccg agccgccgcc cgtcccgctc
    18721 ccgccgccgg aggcgccgaa gggagctccc gcgccccccg tcccggaagc gacgctctcc
    18781 cagttcccga tccggagcga cgggcgcccg agcgcggtcc gccccgctcc cgccccgaag
    18841 gtccgtcggc cgcgctcctc gacgtcgctg tcgacggcgg cggaagcggc cggtctcttc
    18901 ccgcggctcc tggcgaccct cgtcgacggc gtcatcctcc tggcgctcga cctcctgctc
    18961 ctttctcccg tcttcctcgt cttcctcttc aaggacgcct tccagtcgac ggacctcctc
    19021 cgcgactggg ctttcctcgc catcgcgggc ggctgcgcgg tcctcgtcct cggagcgaac
    19081 ctctggtacg tcgtcggcgg ctgggcgcgg acggggcgga cgccgggcaa ggccctcatg
    19141 cgcctctcca tcgtcctcgt cgcctcgggc cgggccgccc cggggatcgg cctcggccct
    19201 gccttcgggc ggttcctggc ctggaacctc tccggggcgg tcctcggcgt cggccacctc
    19261 gtcgcgctct tccggaagga ccggcggacg atgcacgacc tgctggccgg gacgcgcgtc
    19321 gtccgcggtc ggtaggagcg cggcctcagc gcgtctcgag gagagtccgg agcgccgggg
    19381 acaggagcgt ggcctcggca cgggccgagc cccccttgct gcggaacgcg gcgaggctct
    19441 cgcgggcgcg gtcgaggagt gagcggtccc ggcggccttc gaggacgaag cgcccaccga
    19501 gcgccgccgc ttcgacgagg tccagctcgc gggcgtcggg ggtccgcttc cgctccgcgc
    19561 ggactcgctc gacggtcgtc tcgaagtcgc cggcggcgag tgccgcgagc ggcccttcga
    19621 gccggggcgg gaccggcgtc gggacgagga ggaggggcgt cgcggggggc tccgccgggg
    19681 gcgtcgggac gagctccggg gtctcgagcg ccgcggagac cgtcggcgcc ggggtcggcg
    19741 cgggagggag aggcgtgggc gcggggggcg gcgttggcgt cggcgtccgc ggcggagggg
    19801 ctcgccgggc cgcctcgcgc cgttcgagct cgacgatccg gagcctccgg tcctcggtct
    19861 ccctgcgatc ggggaaggag ccgcccgcgg ccgaggcgcg caggtgagac agggcccgct
    19921 cttcctcccc gagctccatc agggcccgcc cgagccagta gtggggatcg tagtagtcga
    19981 cctggtggcg gctcgctccg aaccggagct ggggcgtctg gtggccggtg gagagggcgg
    20041 actcgagctt cggaatcgcc tcccggaacc gccccttctc gacgagctcc cgaccctcga
    20101 ggaacggccg ggcggccgcg tcgcgcggta agatactttg ttgcggtgcc gggaggcccg
    20161 cccgcggggc gaacgccgcc aggctcaggg cggccccgag ggcgacccgg atgagccggt
    20221 cgcgtgaggt ggaacgggcc gaacgacact tcatggcggc gcgccgcgtc gagtttagcg
    20281 gcggggcgcg cggaggggat cacgacgatg cctcgaaatc tcggttccgc ccgcgtggcc
    20341 ctgacggcag ggctcctgct cctcgcgtcc ggcgctctcg cggggccggt cccgtgccct
    20401 ccggaccgcc cggtcctctc cggcccctcg gaagtccacc ggctcgaggg ctactggatc
    20461 acctggacga acgtcctctc gccgcgcggc tccggccagc ccgatacctt cctcatcgag
    20521 cggtcggagg acccgacgtt ccagaccggt gtcgagcgcc ttcagccgac gacccggacg
    20581 acggccacgt ggggcccccc cggaggcgcc ccgcgcacga tctaccatcg cgtcgccgtc
    20641 cagtccttct gcgccgggac ctcgccggtc cgggtcgatt cggccgtcct cgcggtccac
    20701 gtgaccgacc agtgccgccc gacgatcggg ctcccgcctc cgctggcgag ccccgccctg
    20761 ccgcccgcga acacgaccta cctcgtctcg tgggacacgc tcggggccgg ggagccgggg
    20821 ccgggaggcg gcgcgcaggg ccttcgcttc cgtctccggc ggacggccgg cggcgacgtc
    20881 accgagagcc tcaacgacgc cggcgccgcc gcgttcaccg acccgccggg cgagtacctc
    20941 taccaggtcc ggaccgagaa catctgcggc gacgcgagcc cctggtccga gccgctgcgg
    21001 gtcgtcgtcg gcacgacgaa gacctcggcc ctcttcctcg tctcggcccc gcgtcccgtc
    21061 ctgctgccgg tggacgcgcg gggcgaggtc cgggtggcgt tcgtcgtgag gaacgccggg
    21121 agcgagccgc tcgacgtgac cgccacgggc gggggcgccg gtctcacggc cgagcccccg
    21181 acgttccggc tcgagtccgg ccgcgacctg cgggtcgagg cccggctgcc gaaggcgccg
    21241 tcggccgaga ccccgttcca cggatccgtc gtcctcgcgg cggacggcga cgtctcgctg
    21301 cgcgtgccga tcgacgtcgc cacccggggc ctcgacacgg gcggggtgcc gtccggcgag
    21361 cccgacacct cgcgcccggc gggctgggac gaggacgccg tcgacgtcga ccggaacggg
    21421 aacgacgtca cccgcctcct cgtgaacccg ggaggcgtca atgcctacct tgccacgtcg
    21481 gtctccgagc cgtggctcgt cgtctcctcc gccgacggga aggcctggga ccggccgctc
    21541 gcccccggcg agtcgcgccc gatctggctg cgggtcgacc ggagccgtcg gcgggcggcc
    21601 atcgggacgg agatcgcgac ggtcaccgtc gtcacggcgg ggcacgaagg cgagccgaag
    21661 agcctgagcg tgatcgacga cgggccccag gtgaccggcg tcgggagccc ctcggcgaac
    21721 gacgacatcc ctccgctcta cctgatgaag agccggctcc tcttcccgtc cctcccgaac
    21781 gcgctcgacg cgcgcggcgc gggccgtttc acctcggacc tctggctttc gaacgtcgac
    21841 gcgctcaacg cgatccaggt cgtcctcacg ctgacaccgg ccgggcagtc gaacacctcc
    21901 gccggcgtgc ggcagttcgc gttctcgctc ggcgccggtg agacccgccg gttccggaac
    21961 gtcatcggga cgattttcgg gtacgagggc ggctgctcgc tcgacgtcag ctccccgagc
    22021 ccgactctct cggccacggc cctcgtgaac aacaagccag tcgtccccct cgtggccggc
    22081 aagaccgccg cgctcgacag cgcgatcgcg ggaacggtcc tttcgaccgc cgagttcggt
    22141 ttcgagatgc ggccggtcgc tcccggcgaa ggggcgagcc agaaggaccc cgaccacgtt
    22201 gtctccgggc tctggcacga tgcccggcgc cgcacgaacc tgatcctgcg ggagacgaca
    22261 gggaacgaga cgaccgtccg ggtcgagctg ttcgacggcg agggccgggc cgtcctccgc
    22321 gacgggatcc ccgtcgattt cgagagaacg gtgccgggcc acgggtcggt ccagatcaac
    22381 gacgacgatc tcttccccgc cggcgagttc tccttggcgt tccgcgcgaa ggtgacgttt
    22441 ctgcgcggct cgatcgaccc gttcgggcag acgcacggcg ccgtcgtccc gctcgcgacg
    22501 gtcatcgacg cgggcaccca ggacgccgcg ctccgggtcg gtgtctccga gaaggccctc
    22561 acgccggtgg cggtgggaag cgccccggcc gcgagggcgc tggccacagc gagcgccctg
    22621 ccgttcgagg gcgggcccga gcctctcctc ttcccggcca gccacgtcac gggcgcggcg
    22681 ctctcgagcg gggcgccgcc gcgctggagg agccgcgtca cgttctcgaa cgtgagcgcg
    22741 accgagcagc ggaacgtgcg gctgagattc cggcccgcgt ccggagcgct ccccgccgga
    22801 atgctggagc agacgtcggt cgtcgtcccg ccgggctggg ggatcacgtt cgacgacgtc
    22861 ctcgagcagc tcttcggcct cccggcggga gccgaggcgt gggggacgat cgaggccgac
    22921 aacctcgagc gttcggacgg ctcgtggctc ttcacctggt cggacgtcga cgtccagacc
    22981 gagacctaca ccgccgacac ggaggacccc gagcggggcg agttccggac cgggatggag
    23041 ggctactcct accagcacgg ctactcctcg ttccagtcga acctcgggac ggtcctgatc
    23101 gacggcgccg agatctcggc ccggaaccgt acgaacctca tcctgcagga ggtcggcggg
    23161 gcctcgtgca agctcgcggt cggcgtctac cggcccgggg cgctcgtacc gctcgcggtc
    23221 cggatcatcg acctgaaggc ttcggagtac ctctcgcggg agctcttccg cgacctgatg
    23281 cagctcgacc tcgacgagat cgtcgacgcg cgggtcgtcg taaggcagct cgacggggac
    23341 ggtgtcttca tggcgttcgt ctcgaagatc aacctcgtga ccggcgaccc ggcgaacgtc
    23401 ttcctccgcc cggcctctgc aggaacgggg cgctgacggg gagcgggcgg gtgcgcggta
    23461 acctcagcgg ggagaagcgc cccgtggagg agacctgagc ccgcccgccg tcgagaggat
    23521 cgcccactac cggatcgtcc gccgcgtcgg gcgcggcggt atggggatcg tctaccgcgc
    23581 ggtcgacgag cgggacggga agaccgtcgc gctgaaggtg atccgggaga cggaggtggc
    23641 cggggacgag tcggctcctc acgtcgccga ggcccgggtc cgcttcacgc gcgaggccga
    23701 gatcctccag gggcttctcc acgcgaacgt cgtcacgttc cacgagatcg gcgaggagga
    23761 cgggaccccc tacctcgcga tggagttcct cgagggacgc ccgatcggca gctatgccgg
    23821 ccacccctac acggagaccc tcccgctcct gatccaggcc gcgaacggca tggagtacct
    23881 cgcctcccgc gggatcgttc accgcgacct ctcgcccgac aacgtcttcg tcgtcgagag
    23941 cggcagcgag aagaccgtga agctcctcga tttcgggatc gcgaagctct tcgagtcggc
    24001 cacgtcggaa tcgctcacgg cgaccggctt tttcctcggg aaggtcgcgt acggctcgcc
    24061 cgagcagctc ggctcgctcg gccagggcgc tccgctcgac tggcggagcg acgtctacag
    24121 cctcggcgtc atcttctacc aggtcctcgc ggggcggcgc ccgttcgagg ggcgcgcgcc
    24181 ggtcgagtac atcgccgctc acctgaacct ttcgccgcct cccgtcgccg ctccgcccga
    24241 cgcacccgcc ctcccgatgg agctcgtgag gctcatcggg cagatgctcg agaagcgccg
    24301 cgaggcccga ccccagagct accgcgaaat caccgagcgg ctcgtggcga tcctccgttc
    24361 ctccggtgcc ggagtcgcgg gactgctccc cgagggggac ggcgccgcgc cccccggcgg
    24421 gccggccgcc gcgcgtgccg ccacctctcc gacgcagctc gtttcggaga agccgcggag
    24481 cccggccgtc cccgcgcggc gggtcgtcgg cgtcctggcg gcgggcgcgg cgctcctcgg
    24541 gctcctcctc tggggggcgc tccggagcgg gccccgcgaa gcgccgcccg cgcgccccgc
    24601 cgacccggtc cacctgccgc cggcaacggc ggagctgcgg atcacgtcga tcccgtgggg
    24661 cgtcgtcgtc tcggtcgtcg acgtggcaac gggaaaggcg gtcgcgttgc ccgcttccgc
    24721 cgacacgcca ctcccgctgg ccgttcctcc cggacggtac cgcgtcacgg tcgtcgagcc
    24781 ggctcccggg accggccgtg ccgagacgga cgcggaagcc gccaccggaa gccccgccct
    24841 cgtcgcgctc acactgaggc ccctagaaga gatcgtggag accctgcgat gaagaagccc
    24901 gtccttgccc tcgccgccct gctcctcgtc cggatcccgg ctccggccca ggtcgagtcg
    24961 gcgctcctgt cgaaggtgac gttcaacctg acgaacccgg gcggaaagtc gctcgcgatg
    25021 gggggcgcct tcaccgcgat cgccgacgac gcgaccgccg ggatcgcgaa cccggccggc
    25081 ctcggcctcc tctcctcgtt cgaggccggc gtctcggtga agcgggtcga cgacgtcatc
    25141 ggcctcgtca cggcccggtc gacggcgacg ggtggcctcg tcgagcccta tccggacccc
    25201 cgtgccgcca actccgacat ctcgggccgc agctcgctcg tcgagttcgc cgggatcgtg
    25261 atccccgtct cgcgccgggt cgtcgccgcg gtcagcttcg ccgagaacct gagcttcgaa
    25321 ggagaccccg gtccggacgg ctacacctac atcgagctcc gcgacaaccg ctcggggggc
    25381 tccacccgga acgactacct gttcgaattc cgcgagcacg gcgcggtctc gctccggaac
    25441 cgcgtcctgg ccctgtccgc cgcataccga atgacggacc gggtccggct cggagcgggg
    25501 ctgaccgtca accggatgga gctgaatctc ctcggtgacg agacgggggc gcaccggatc
    25561 gtccacagga cgttcacgac gccgacggtg atcgagacga ggaccacgac gatggcgatc
    25621 cgggacctcg accggagcgt gctcggcttc gtggccgggc tgcacgtcga cctcgtcgcg
    25681 ggcgggaagc tgaccgcggg cgccgtgtac cgtcagacgg cgaaggcgaa cgggacgctg
    25741 gagatcggcg gagacgtccc ggtcccgctg gccggacagc agagccggcc gttctcggcc
    25801 cgcgttcccc gcgacgcggc ggtgggcctc gccgcccagc cgattcccgg actgacggtc
    25861 gcggccgagg cgcagtggat cgcctacggc gacttcatcg accgctccct cccgaccgtc
    25921 tcgtacgccg gactcgtggg gccgctcccg ggcatgttcg tgagggacct cctcgccgag
    25981 atgaccccgc cggacgacgt cgtcgtcccg cgcctcggga tcgagtacgt cgcgacttcg
    26041 gggagcctcc tcctcgcgtt ccgcctcggg taccaccgcg agccggcgcg cggcgtcacc
    26101 gcggacctcg ccgcgatcga ggagacgacc gggatgccgt tcgacgtcga cgagccgcct
    26161 ttctccggca gcgtgaggac ggtgtacgac ggcgggcgcc ccgacgaccg cttctcgggc
    26221 ggcctcggcg cgacgatctc gcggaaagtc tcgttcgacc tcgcgttcga cctgggccgc
    26281 tcctctcgcg tcctgtcggc ctcgctcttc taccgcttct gacggcccgg cgcgcgaggg
    26341 gcgcgccgga gccgagccgt ttcgcgcttc agaccggggc gccgcaggcg gggcaaaaaa
    26401 cgtccccgtc cgccgcctcg tgcaggcaga tctcgttccc ggccggcgcg ggcggaggcg
    26461 ttgcttcagc cttcaggacg agccacgcgg tcccgtcccc cgtggaggag acctggacgc
    26521 ggacccgccg atgtgcgcgt tccaggagga ggcctccctc gtcgaacggg ccggccggcc
    26581 ggcttcgctc gacgagccag gcgaacagcg ggtcgagaac cggggcgggg atcggccgag
    26641 aggagaggac ggcgccgccg gcgtagctcc tcacgacggc accccggccc tccgcgccga
    26701 tctcgaccgc ttcggcccct tcgtcgaggg cgtcgacgag gagcgcccgc gcgaggcgcc
    26761 cgaacgggtc ggtctcggcg gaacgcgccg tcttcgtcga cggctccgca gtcgcggcgc
    26821 gcgggcgatc gccggcggcc ggcgcgacga cctgttcggc cgccgggcgc gccagatccg
    26881 actcttccgg tgcggccggg ccaggctctt cccacctcgc caggaggtct tccagaatcg
    26941 gttcggccgt cacgtagagc cgaacgtcgc atccggtcga agcgacgaga cccgtctcca
    27001 ggacggggtt cccggggtcg acgacggcca cgtggaggac gtcgccgcgg cggtcgaacg
    27061 ggagcgcgcg gaggcgccgt cgcgtgtcgg cggggagggc ctcgacggcc tcgcggctcg
    27121 ccgcggcgag cctctcgcgg gagacgctcg gcaggcccgt cagccggcag agcgcgtcga
    27181 ggagcgtctc ctcgcggacg gctcccgtct cgaggagcgc ccggtcgatc gtcccgccgt
    27241 ggacgcgcct ccactccgac gcgcgccgca ggcggcggaa ggtgaggtct ccagactcgg
    27301 cgaggatctc tcccagcccc cgcgacacga gcgctcctcc gttgggcgaa gagtacacgg
    27361 gagcggggcc ggcgcgaccg gccccttcat cggtcgtccg gcctcagccg cgggacccgc
    27421 agcttcccac ggcgccggcc gccatccggg cctcgtcggg ccggaaggcc gccacgagct
    27481 tctccgcgac ggcggagagc cggtcgcggg tgccgacggc cccggcggcc cgctcgaagg
    27541 cctcgaagtc cgcaagcatc ttctcgaact cctccggggt gaggacccgc gtcgacatcg
    27601 ggtagaggat gttgtcttcc ttctggatgt gctggcgcag cagcggcacg aaaccgcgcg
    27661 aggcggcgag gaacgacgcc cgctcctcag ccgcgaccgg gccctcgccg gcgccgaccg
    27721 cggcgatccg cccgacgagc tcgcgcccct gccggtgctc gtgcagcatg accgcaacgg
    27781 gtccggcctc ggccgagaag ccgcgctcca ccatccgccg gaagaggatg tcctcctcct
    27841 tgccgtggtg ccaggcgtcg gcgaagccct tcaggaagcc ggcgtagtcg cggacgacgg
    27901 cgcgctcgag cgcgaggcct ccctccacct cgatcgcgca ggtctcgagg gagccgaggg
    27961 cctgctcgat gatgcggtgc tcgtccatca ggatcgtcag gggtcgggac atcacgtctc
    28021 ctccgtcggt ttcgtcgttc tcaatggatc tgtagatccg ttgatatggc aaaaaaaaga
    28081 gaggctcacg ccgcgctccc cgacccgccg aggtcgcgga gggtcacggt ccggagcatc
    28141 cgctcgtact cctcccggac cttctcccag tggggatgga gcgggcaggg atcgtcggag
    28201 tcgcagcggc cgaggccgaa agcgcaggcg tccttctcgc gcctgccgtc gaacgtctcg
    28261 aggacggccg agatcgtcgt gcccgccccg gtccccttca tccggaagcc gccgccccag
    28321 cccttctggc tgacgacgaa gccgcgcttg cggagctggg cgaggacctt cgagaggtag
    28381 ttcggcggga tcccggtctc ctcggcgatc tcgcgggcga ggacccagcg ctcgcggtgc
    28441 gccgcgaggt agccgagcgt ccggagcgcg tggagggagg tccgggagaa catcgcgtcg
    28501 tctctcagtg gatgaggtta tccgttgaga cggtcccgcg caagtggcgg cgacccgtcc
    28561 cgcggtcgcg cggcctcaga gccgcgctcc ggcgcctcgc gcggggaagt actcccgcca
    28621 gcggcggtcg acgagggcga ccgtcgccgg gtcgggcgta acgacggcgg gcatccagct
    28681 cttcatccgt gcgtcgagga cgatcggcgc cgtccgggcg acctggtttc gcacgacctc
    28741 ggtcctcgcg gcgtggacgt cggcccccgg ctcgaatcgg gtgaagacgt gccagaggaa
    28801 cgagcgggcg tctctcgccg cggtctccgc gtcgtccacg agggcaacga gcggccaggc
    28861 ggcgacggcc gggtcggccg cgaggcgcgc ggcgtagtcg cgatcctctc tccacgaagg
    28921 ccccgagacg cagagcgcgc cgccgcagaa gacggccgcg gcgccagcgc ccgcgggaag
    28981 cgtcccctcg aacgcgcgcg gcaggtcgcg gatcggctcg ccgagccccg cgaggacccc
    29041 cttcgacccc tcgttcactt tcgggccggt gtagtcgagc gtgtccatcg cgacgttcga
    29101 gacgacgaag aggtcggtct cgaagcggca gcgggagagg accgtctcga ggagcgcgcg
    29161 gaagtcgtcg aggtccacgc gctggtcggt gagaaggagg aacttcgtca gcgcgagctg
    29221 cccctcgccg aggatccgga acgcggtcga gatcgcctcc cggccgtagc ggtccttcac
    29281 aacggccgca gcgagggagt ggaagccggt gtcgccgtag gtcttcaggg agacgacgct
    29341 cggcatgacg acggggaaga ggggagagag gagctcctgg aggaagtcgc cgatcaggaa
    29401 gtcctcctgc acgggtttcc cgacgacggt cgcggggaag atcgcgtcgc gccgccggta
    29461 gacccgctct acgtcgagga ccgggtagtc gtgcgcgagc gagtagtagc cgtagtggtc
    29521 accgaacggc ccctcgaggc ggcgcacccc cggcgggacc ttcccggtga tcgcgaattc
    29581 ggcgccggcg acgagccggt gcgggtgccc cggcacgcgc gcgagcggga gcctcttccc
    29641 gaggaggagc gaggcgagga ggagctccgg gagtccctcc gggagaggcg cgatcgccgc
    29701 gatgatgagc gcgggcggcc cgccgaggaa gaccgtcacc gggagcgcct cgcccttcgc
    29761 ctcggcgacg tggtggtgga agccgccccc ttttccgatc tgccagtgga tcccggtctg
    29821 cctcgcctcg tggatctgga tgcggtacat cccgaggttg tgctcccgct tctcggggtg
    29881 ctcggtgtag acgagcggga gcgtcaggaa cggaccgccg tcctcgggcc actgcgtcag
    29941 cgccggcagg cgggtcaggt cgggctccgt ctcgacgacc tcggtcacgg gagcggcgga
    30001 gacctccctc gtccccaccc tgagcgcctc gcggaagaac gagcggaagc cccagagctt
    30061 cccggccgtc ggcgggagga gctcgtgcga ggcgcggacg gcggtcttca cgaagtcgag
    30121 cggcctcttc ccgaacgcca ggtcgatccg cttcgccgtt ccgaagaggt tcgtgacgac
    30181 ggggaaggag gagcccttcg gacgccggaa cagcagcgcc ggccctccct gcgcgatgac
    30241 gcgccggtgg atctcgggga tctcgagcct cgggtcggcc tcggcctcga tctcgaggag
    30301 gtcccc
//
LOCUS       DAOONL010000034        29913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1347_length_29913_cov_16.291915, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000034 DAOONL010000000
VERSION     DAOONL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 29913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29913
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_29913_cov_16.291915"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLP50_07285"
     CDS             complement(<1..433)
                     /locus_tag="PLP50_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51390.1"
                     /translation="MTKTTSILKVAFRAMGRNKMRSLLTALGIIIGVACVVATIGIGE
                     GARVQTEQQLQSLGTNYLMIHPGTVTSSGARSGWGSNSKLSEEDVDAIRREVPTISYV
                     SASIRSVAQVVYGNQNWSTSIQGGEVDWPAIRSWNVAQGQFF"
     gene            complement(430..1107)
                     /locus_tag="PLP50_07290"
     CDS             complement(430..1107)
                     /locus_tag="PLP50_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51391.1"
                     /translation="MGETEVRALDGVTLDVAPGEFLAVMGPSGSGKSTFMNILGCLDR
                     PTSGTYVLEGVDVSTLDRNERAGIRNDKIGFVFQNFNLLSRTSALENVELPLLYSRKH
                     RLSDAEQLVKARECLGRVGLADRWDHTPAQLSGGQQQRVAIARALVNDPSIMLGDEPT
                     GNLDSRTSDEVMGIFQALNEEGKTIVLITHEHDIAEYARRVVAFRDGRLVADELVKAR
                     RSAKGAR"
     gene            complement(1170..2465)
                     /locus_tag="PLP50_07295"
     CDS             complement(1170..2465)
                     /locus_tag="PLP50_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51392.1"
                     /translation="MKKALVGAAIVAAVVVAAVLLLSKDKKSGPKFRKEKVTKGSVVA
                     TVTATGTLSAVTTVKVGSQVSGIIARLHADFNSEVRRGQLLAELDPTPFQATVDQRRA
                     DQERAKVELRNAELTLARAKKLLEAQLQAQSEYDNAKANRDSAAASVAQSSASLKQAE
                     TNLAYTRILSPIDGVVVDRQYDIGQTVAASFQAPVLFTIAQDLTKMQVLTNIDEADIG
                     RVKEGQEATFTVDAFAERTFTGRVSQIRLSPQTVQNVVTYPVLLDVGNPELRLRPGMT
                     ANVLVPVDRRDDVLRVPNAALRFKPDPADLADGGKKKVDAAAAPGAPPASGAGSATAG
                     APGAGQPGRRDGGQPGGNGAGRSGAGRPGPSRSGEVYVELPTGKLRTVRLRTTLTDGN
                     LTAVVTDELKEGDEVVLGLATARAAGNAGGGAPRGMGRM"
     gene            complement(2470..3537)
                     /locus_tag="PLP50_07300"
     CDS             complement(2470..3537)
                     /locus_tag="PLP50_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51393.1"
                     /translation="MRVLRSIQVLLPSLLLAASPAPAESPAETMPRFVLGLALSDLEP
                     LRDREMRDGELLFAFLSAELERDGWGARTELRAREGRFRDYFPGPVWLEEGYAWVGTP
                     LGELRAGKVPSVVGLADETFGGTLFSLNGVNRNPDWGVQIVGSRPLGWHSLDWAARWA
                     GSNDRVAWEEDGKGVESDPAARLRDGLSGRASFLYNRGLWTVRPGLSGATARIVPTDG
                     RREYRRSDVSGDVTATLGPISLQVQGLLRRADDPAGGEVRPRAERDGRALLFGAGVEF
                     PESTFRYVYSEWRYDGAASNERIHQPAAVWKGRKGIEAIVEYSSRRIRTGETVRTFNA
                     FRLGLVVRFPFGDSTGRGAKG"
     gene            complement(3534..4454)
                     /locus_tag="PLP50_07305"
     CDS             complement(3534..4454)
                     /locus_tag="PLP50_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51394.1"
                     /translation="MDVVLPRVVLGLASLALARRARSPRGAALAGAAGAAAAAGASLP
                     ALPAPLAFGSVLLVGILVLTALRADLHSPLVSEAAASSLAIAGGAGAACALALAGAGV
                     GSASAGAGAVFAALSVWGAEGARASAEAGWRRGAAWASGAVLSTGVAAGLVALADHLP
                     PRLSFLPAAAASLVSILAWLPALVLERLRVRRELGEEARLGILPEEDLPVLGNPWRRR
                     REPRLGRADERHEYVRSALLLAVARQQQRRRTGEAVRLRQLEVLAFRTRVRRAVEGRG
                     ARFAGLSGDAEAWAGAAPEAARGAPYHPEA"
     gene            complement(4457..5401)
                     /locus_tag="PLP50_07310"
     CDS             complement(4457..5401)
                     /locus_tag="PLP50_07310"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPA51395.1"
                     /translation="MNVYPDPLRRSDLPRGGVLTIGNFDGVHLGHQAMLRAVVARARE
                     IDAPALVLTFDPHPTTVLHPQRAPKLIQTLRQREEAIAALGVDGLVVVPFTKDFAAMP
                     AEDFVRDLLGRCLGAREAHVGSGFAFGCGKRGNVALLSRLGEESGIRVATLDVVSDGE
                     GPVSSTRVRTLVEAGKVSEAGRLLGRPYAMEGVIAKGDRMGRKIGFPTINLRSDNELY
                     PRDGVYVTEVLLRSFERTFSCVTNIGRRPTVYEDYATTIESYILDFSSDVYGEPIRLS
                     FHERLRDELVFPSMLELTAQIRRDVDATRLWFLSRGRG"
     gene            complement(5415..6767)
                     /gene="rimO"
                     /locus_tag="PLP50_07315"
     CDS             complement(5415..6767)
                     /gene="rimO"
                     /locus_tag="PLP50_07315"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPA51396.1"
                     /translation="MSLKLPSVGIVSLGCPKNLVDTEVMLGYLKREGLELGDPDSSRV
                     VIVNTCGFIDQAKEESVNAVLEQVRRKEAGEVDRVVVAGCMVQKYGAELAAEIPEVDH
                     FLGLDELEKAPAAALGLPSLARFAAKPLATRLYDDLAPRVLTGRRGYAYLKVAEGCNN
                     PCTFCTIPQMRGLQRSRSIESLVREAQGLEAQGVSELVLISQDTTRYGEDLGLGREGL
                     ATLVAALLRETGFPWVRFLYAYPKTLHDSVLELMAAEPRFVAYVDVPLQHVSRSVLAG
                     MRRGGDPESCLRLVERLRAAVPGIALRSTFIAGFPGEGEAEFAELLAFVKAAELDHVG
                     VFPYSPEPGSGAEGLGDPVPAPVKGERCAAVMEAQREISRRKNRHLRGKVHDAILEGA
                     STESEMLLEGRLARQAPEIDGKVLVNDVPEGFVPRPGAIVRVRVTGAHDYDLVGRVVP
                     "
     gene            complement(6865..8922)
                     /gene="aroF"
                     /locus_tag="PLP50_07320"
     CDS             complement(6865..8922)
                     /gene="aroF"
                     /locus_tag="PLP50_07320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPA51397.1"
                     /translation="MSDLPNVRAALDRIDAALLDLLAERAAVVDEVAALKSREHALLL
                     RDLERERDLLSKVGRDAQRRGLNAFHVVKIFRDVVEASVRQQESRLTRAANGIDEEPA
                     LLRVAFQGTEGAYSHLAGKKFFADRSEEPIFVGYRSFRQAVEAVEREECEFAILPLEN
                     SVAGGINETYALLGTSKLSIVGEEVWPVEHCLLGLAKVPLARIRRVFSHPQALMQCSE
                     FLESLAGATAESWFDTAGSVAKVKELGSEENAAIASEEAGERHGLVVLRRSIANQRNN
                     QTRFVVVHRRPFRFDPRIPCRTSLLLVTDHREGALERCLAELAKAGVNLTKLESRSMP
                     ATPWEYQFYVDVEGNVDDPRLAEALDGVGRNARYLRVLGCYPRKADPSREVPKDVDLL
                     PGAAPQVSWPAGTPVPVESPVPYPLAARRATDPGARTPVKVGDVVFGEGFVVIAGPCA
                     VENEAQLFEAARVVREGGGRVLRGGVFKPRTSPYSFQGLGIPGLDLLCRAGLEYHLPV
                     VTEVVAPEDVEKVAEFADMLQVGARNMQNFALLKAVGQSSRPVLLKRGMMSSVEELLL
                     AAEYVLSAGNRAVVLCERGIRTFETSTRNTLDLGAVQVLKARTHLPVIVDPSHAMGVT
                     AFVAPMARAALAAGADGVIVEVHPHPEEALCDGPQALTPELFATMMGDLRRMAQALGV
                     KMA"
     gene            complement(9011..10318)
                     /gene="asnS"
                     /locus_tag="PLP50_07325"
     CDS             complement(9011..10318)
                     /gene="asnS"
                     /locus_tag="PLP50_07325"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPA51398.1"
                     /translation="MSTGPVASVSGLRSRIGETVTLRGWLYNRRDSGKIRFLVFRDGS
                     GYVQCVVVKKEVSEEVWTESDRLTQESSFEVTGVVREAPRQEGGVEVGVTGLTVLSVC
                     HDWPITPKEHGVDFLMSQRHLWLRSAKQVAILRVRSEVESAIHDFYHSRGYTRVDSPI
                     LTPNACEGTSNLFETQYTEEEKAYLSQSGQLYLEPAAAALGKVYCFGPTFRAEKSKTR
                     RHLREFWMVEPEVAFLEIDGLKALAEEFVKELTARVLDRRKEDLKRLERDTTVLEATV
                     AKPFPRIDYRDAIGILEKKGFPVKFGDDLGGDEETALTEGFDTPVMVTRYPAAIKSFY
                     MQPDPEDPEVVLGLDMLAPEGYGEIIGGSQRIHDHDLLLSRIRQHELPVEAFQWYLDV
                     RKYGSFPHSGFGMGLERFTAWMCGVPHLRECIPYPRMLYRIYP"
     gene            complement(10592..10843)
                     /locus_tag="PLP50_07330"
     CDS             complement(10592..10843)
                     /locus_tag="PLP50_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51399.1"
                     /translation="MANPAWKTASDVTEGYLTLNSVMLRKYEPHELLSLQAELEKAAR
                     ELRGTVVPVDDVDGNQKKNRKLLRISQALTVLQAARSRR"
     gene            10950..12137
                     /locus_tag="PLP50_07335"
     CDS             10950..12137
                     /locus_tag="PLP50_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA51400.1"
                     /translation="MAFQGVDFYQLDGLLTEEEIAVRDLVRDFVDNEVLPVIEDCAYE
                     GRFPAELVPKMAAMNLFGPTISEYGLPGLSNVAYGLITQELERGDSGLRSFASVQSSL
                     VMFPIWKYGSKEQKDRWIPALAKGEAIGCFGLTEPDFGSNPGGMRTVAKRDGDSWVLN
                     GAKAWITNGTIADVAVVWAKVGGPEGRVHGFLVEKGTPGFTTSEHRMKMSLRASVTGQ
                     LAFEDCRIPAENVLPGVDGLKGPLSCLTQARYGISWGGIGSAMATYTCALDYAKSRVQ
                     FMGRPIAAHQLVQEKLAFMITEITKGQLLALQLGRMKDAGTMKPEHVSMAKRNNVWVA
                     RECAKLAREILGANGIVGEYPVFRHLANIESVFTYEGTHDIHTLVLGQAVTGIPSYNP
                     PAE"
     gene            12224..13714
                     /locus_tag="PLP50_07340"
     CDS             12224..13714
                     /locus_tag="PLP50_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA51401.1"
                     /translation="MAITGNLRTMQLSELLQWLSMGQKTGTLAIRGGAGEKRIYFQNG
                     RIISSSSTIEREYLGHFLVSYGYITEEELTKAMEVQEESKILLGKILVMIGAIAEEDL
                     VELIRLKACETIYDIFLWPEGAFQFLDGEIPQRPMVPIAIDVTGIVMEGLRRFDEWQR
                     IRGRIRSLRDVPTIVSPVDVESLTEPHRLILRAIDGVRSLAEIATATHNSDFPVARLV
                     FDLIESGHVELASAPTVTAPKSSELTPEDPYETVFEEAATPHFEGVSDTADELPLPPP
                     PPEGRPAPPRDFARFLRRGPDSGSHRVRPSGPVTTSLAAPVAAPPAAPPPSTSFRAAP
                     PAPSPAGPPTSGSLRVGGPSAASFLDIGAPPANVPWPAPVSGAFRVPAPSGPPSGSLR
                     APAPQGPPSSASLRSSAPPPSSQNRLEIPASASGSTFAPTAIPSLTRPMEELMSWSFT
                     PNEAFILSRINGLWDVRSIAKISPFPEGEVLRVFEKLHDGGLIRWR"
     gene            13730..14983
                     /locus_tag="PLP50_07345"
     CDS             13730..14983
                     /locus_tag="PLP50_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPA51402.1"
                     /translation="MEPPLPSPLLLAATAAAFAAACAIAFRAGRRTVAATLRQELLEA
                     ERRGEAIAEEGRLRSARLERANAAQARRAAALERVLRATPELRAHLPLDSVLRNTVQA
                     ARKSLGHRQVLLSLHDRLEGTLVPRAHVGLEDEWPSLAEVRVPAGPLAHDLVAPETAE
                     AAPLRSLRRRLGLAPPVAMPLAAGSRVVGLLELAEPGSDAVDVEEDRVVLDLFALQAV
                     QAVRLARAYRTVRLGTLRDALTGVANHGSFQETLRHEIARHERSAESLVLLMVDLDDF
                     KAVNDRFGHPVGDAVLRGVVSRLLESVREMDVVARYGGEEFAVVLPQTDAAQGSRVAE
                     RLRAAVAAAPLPAGPAEPLPLTVSIGLAVYPEDGRTKGALVECADRALYAAKRTGKNR
                     VVRFTRVPAASSDLRPSLRTAPRGA"
     gene            complement(14916..15572)
                     /locus_tag="PLP50_07350"
     CDS             complement(14916..15572)
                     /locus_tag="PLP50_07350"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPA51403.1"
                     /translation="MPRSRQAGRPSREFWERTTEEVARALLGCRLVRRRPGQPPVTVV
                     VVETEAYLGVRDRAAHTWNGRRTPRVAPMWGPSGHAYVFRVYGMHDCLNVVTREAGVP
                     EAVLLRAAVPLAWWTGTPVARGELLAASGPGRLCRVLGIGRELSGASLGGPELELLLP
                     LPGAARPLLVGPRVGVDYAGDAARWPLRFAVGGCGAVTRRAELSEGSGGGRWRPPEPA
                     "
     gene            15619..16569
                     /locus_tag="PLP50_07355"
     CDS             15619..16569
                     /locus_tag="PLP50_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA51404.1"
                     /translation="MPGCPVLVTGGAGFIGSHVVDALLALGHRVVVLDDLSTGDLRRV
                     PAEARFVRGDVRDPGLDALLVEERVDTVFHLAAQIDVRKSVEDPVFDAEVNVLGTIRV
                     ALAAAKAGVSQVVFSSSGGAIYGDPVGDRADEEHPLNPCSAYGVAKLAGEKYLAALAP
                     DGGYLLTVLRYANVYGPRQDGRGEAGVVGIFMNRLLSGQDAVIHGDGLQTRDFVHVAD
                     VVAANQASFLRRVGGTFNVGTGIETSVRELYGLVASACDADRPPRFDPAKPGEQRRSV
                     LDVGRARKLLGLAEFVPLEGGMHATAEWFRLHRAGQGLGG"
     gene            complement(16593..17849)
                     /locus_tag="PLP50_07360"
     CDS             complement(16593..17849)
                     /locus_tag="PLP50_07360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPA51405.1"
                     /translation="MDYRSLLKNVEKTLSAIEESADVVSTVASVGETVVGNFRNELGI
                     IGGRLYELEDGEYVLRRGFGRSKKVSEGMTVPVSYEPIRRAIEEGVVLADLKDPWVDR
                     ELEARLGVTRFAAVSFGDDRFILSFDVHPRVAADDLLVSLGIIRTVLDSKVKSEQLED
                     LLEEARRIQQSILPRHVPEMGDYEVWAATEPAEAVGGDFYDFITLGPEAFGTAIADAS
                     GHGLVAALLVRDVYVGLRMGIKGDLKIARAIERLNRILNKSRLSTKFVSLFYAEFEAN
                     GEVIYVNAGHPGPIHLHARTKSFSEMESTGMVLGPSPNAVYGRRAAKMEPGDLLLLYT
                     DGIVEAHDLRGREFGLDRVRRILLDYRKRSARETVEKLMSTVRLFSSGRAAEDDRTVV
                     VIRRTGKPRGAPASEPPAVTTSASAD"
     gene            complement(17889..19382)
                     /gene="dacB"
                     /locus_tag="PLP50_07365"
     CDS             complement(17889..19382)
                     /gene="dacB"
                     /locus_tag="PLP50_07365"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPA51406.1"
                     /translation="MTFVLLGLALCVARADAGTASAKRTPRRPAAAAPVRPLTLEERL
                     LSVARRTPVRSRSQVGVMIAPVGGAPILAMNGETELILASTTKLFTTAAALDRLGPGY
                     RFRTTLEQDGELLPDGTLSGRVVIRGGGDPAISGRLYDDDPWAVFRPWSAALSARGIV
                     AVRDGLALDTSFFDDVTTHPDWPAAQEQHWWQAPVSALSYNDNVVLVRVTGGLRPGGR
                     ASLGFYPAHPFLMSLVGRVTTHKGRGARVGVSRNPGSPRVVASGAVGRRRTWSGDVTV
                     VDPPLYLASAFTKVLEDEGITVGGEPEVRTERRSAPMSLPLLHVHETPILPTLAVSNK
                     RSQSFFAEQILKTLGAERRGLGSWENGLAEVSEFLRSLGLDPARYRLADGSGRSRENR
                     ATAAAYLDFLQALATRWPHFAAFEPTLAVTGDMAGSLRYRMLGPDTRGKVFAKTGNIA
                     GVVTLAGWIEARSGQRYAFVILANGGCGEGRGHAYQDRILATLARFG"
     gene            19460..22033
                     /locus_tag="PLP50_07370"
     CDS             19460..22033
                     /locus_tag="PLP50_07370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPA51407.1"
                     /translation="MPIRVDLASKTVLLSPAALLAPTSRRIGDGRGDSMERRWIGQAV
                     HRRVLSEALASIPGYAVEKAVRHTFAVDGFTAVLEGRLDGRFEDPDGTVVVDEVKSVH
                     FAEELARLAGSPRLDRFARQLQWYLLAIARAEGRSVRGRLVLADIETGDTRIVPVEWD
                     EAEVEEDLVARVRAVLRDFFGELALAEAKAAEADDLVFPFGRYRPGQREMAAAVARAV
                     RQGEQLLVEAPTGIGKTAAALFPMLVEALRRGRKLYVLTSKTTQQEIFRRTLEAISAA
                     SARSVRLRAKERMCANGVVLCHEDHCAFAKDYAAKLDASNLVDRLLGVSPHLEPDLVF
                     EESRSEQVCPFEVSLELAERADVVIGDYNYAFDPVVALSGLRDPASLDGALLLVDEVH
                     NLVERGRGIWSPEVSEAEVRRLSRAIGTTSPRTAAAAQEAAEEVLALVLDAASALPPS
                     TDAWNPAVDLVPLDLDRLDDVRLSMESLLVRHLADLRNGAERVPDDPVLGLYFLYAGF
                     HDVARRAAPNGRLLEAFDLLASRGSDGAKLSFLCKDPSRLLGETFDAAFATVGMSATL
                     QPPEFYRDLLGLDPDRTSVLSLPSPFPKENRTVLVAPGVDTTWKRRAAAMPRIAALVA
                     EIADACPGNVLALFPSYRFVDDVRALLPPLPGHRVLRPSDRSTELERNAVLSALREEG
                     SRILLLAVSGGPFAEGVDYPGEMLHGVVVVSPALPQVRFEQERMRLYFEERFGRGFEY
                     AYVIPGMTRVVQSAGRVIRSETDVGVIALVCRRFLQKPYRAFLPTHWYDEAPDELSSR
                     EPGEAVRAFFARTTPIDAAGPHAIADPDQGRGRLCGHEAPVRRLRRRPPRSTPASDPS
                     T"
     gene            22036..24189
                     /locus_tag="PLP50_07375"
     CDS             22036..24189
                     /locus_tag="PLP50_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA51408.1"
                     /translation="MKLPAKLRRLASAARLARLRRRFLGAGSAVDHWDELARAGEAGA
                     GRQWTELAQVQEVINERISGEAHVNPYLHFMRRHLAGRLPVARALTIGCGTGELERGL
                     FRHGFAIEHDGFDIAPEAVRGAIAAAKAEGCRGIRYSVADANALLLEPDSYDVVFGVH
                     SIHHVERLENAFEQIALALKPDGLLFLNEFVGPSRFQWSRRQLEVIDGVLRLLPERFL
                     RSRVRGTIKKRAPRPSVRRMLATDPSEAVRSDEILALVDGWFDVVEVRPYGGTVLHPL
                     LDEIAGNFARSEDGGSEILAAICRLEWALIQAGDLTSDFAVVVARKRGGVQAGARPGG
                     RTQAASPRLPDRYEVAEMNPMNVFRRLSGKPGKQADGTQESYKEWWTASSASESGAYS
                     TTYVADGEADYRSRGWNGDANSFGARQLIEIAGLTPSSRVLEVGCGMARVGREMAPKV
                     AEWHGADISANMLEFARARTSHLTNVHLHELSDVSLSAFADASFDFVYITTVLMHLDK
                     EDVYRYLLESHRVLKPGAMAFFDTWNLLHPDTHRIWRAIQADNVGSSKLRGRIQFSTA
                     PELRRNLEEVGFEVVRLDEDRLLRAFARKREAYAYDPSDTLPPFGYVDEPRNESTVSG
                     SLEVGGWVLDGIESVEIRLDGVRSLGRARLGLPRADVAELFPRYRDAAACGFGASVPT
                     EGLPPGSHTLQVIARDRDGFETDLCGNYRSVTFAS"
     gene            complement(24279..25961)
                     /locus_tag="PLP50_07380"
     CDS             complement(24279..25961)
                     /locus_tag="PLP50_07380"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPA51409.1"
                     /translation="MKLNALVLPVTAAVLLTLGAPAFAQCPEEGCPAQTLGTIEAPAE
                     GALVSGFVRVTGFALNGNLVSNVDLFIDGTDEANRVTPAGGANINLPRPDVTQWLPQY
                     AGTPGEQPGFEMSFRAANYANGTHTLHVRITDVTGCCYFLAPRTIRIDNTKNQAPFGI
                     LDVPLPDSSVHANGVLAVAGWALDDRRVEHVDVLIDGLLEREAVLGINRPDVAAFYPN
                     VADASTSGFLVFIDSTRLTNGVHTLTVKATDDQGQQGLLAIRRFQVFNNAPNLPPFGD
                     VDQPMLQATWFGNCLRLPGGPSGGEIRDTRYVMFVSGWALDTSVYAERGGVSHVFLEM
                     DGVVLKDTRGAFNTLGCRREFNLDNALVDCYGFYRPDVNVFYPGFPQSANSGYFFAVD
                     VGHLLTEKGFKEGNHMLQVKAADKEDNVTLLKELPVHIECATQNLDPSPLGYVSDPMN
                     YQFVGGIHPVLGWALDLDGVIRVRVLIDGVPQIDAVTGRDYAEYGLPSPDVSAVYPNY
                     PQRTNARWRFYLDTTKLANSEHDLLVEVIDGRGNYRSAGTRRFIVNNNTLTR"
     gene            26213..27271
                     /locus_tag="PLP50_07385"
     CDS             26213..27271
                     /locus_tag="PLP50_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPA51410.1"
                     /translation="MSKAASNALRVALSLGLAAVLLWLFFRSLDLAELGRALSDARPA
                     GLAAGLAITVVNLPLRAWRWIRLLHHVERVSFRESFVATTIGFAASVLLPARAGEIVR
                     PAVLSRRTGLPFAPALASIAVERLIDLVTVVLLFVVYAAGGWAPTGLAPEAQARLELL
                     RRAALLVGAGALAVFAGLGLLAARPALAERVLAPLERRLPKRFAARVVSLLRSFLGGL
                     AAVRTGRDVAVLAVSSLVMWLLNALQFHVVAGAFGIQLAFPISFFVMTWAVLGLAIPT
                     PGGVGGYHAAVAYALTGFYAVAAAPAAATALATHALAFVPITVAGAVLVATSGLTFRR
                     LAASGSSGPEPSGSSLRP"
     gene            27268..27747
                     /gene="nrdR"
                     /locus_tag="PLP50_07390"
     CDS             27268..27747
                     /gene="nrdR"
                     /locus_tag="PLP50_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPA51411.1"
                     /translation="MKCPFCGGVSDRVVDSRESREGEVIRRRRECLACLRRFTSYERV
                     EESPLVVVKSDGRREEFDRVKVLRGLRRACEKRPVEEARLEALCDEVEALFPTREPRE
                     LKTREIGAFLMEKLKEVDQVAFVRFASVYRRFEDAGDFVEEVETLLSDARDASRRSR"
     gene            27744..28157
                     /locus_tag="PLP50_07395"
     CDS             27744..28157
                     /locus_tag="PLP50_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HPA51412.1"
                     /translation="MTKGRPTTRWIYGSGAGDPGTPPPHLPPVDVVEEVHGWRLVFEI
                     PGTDPETVRVDVAGRVVVVRGERRPTERAHGTFLCVERSAGPFERAIELPDEPDPDGA
                     SASYADGLLTLEIPRRPATRRRSIPIRPARGPEKG"
     gene            28161..>29913
                     /gene="lon"
                     /locus_tag="PLP50_07400"
     CDS             28161..>29913
                     /gene="lon"
                     /locus_tag="PLP50_07400"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPA51413.1"
                     /translation="MAEETASVETIQIPDLLPVLPLKDVVLFPYVIVPLSVSRDRSIA
                     AVDAALSEQRILLLLAQKDPSVEEPTPADLYETGTAAAVMRMLKLPDGRIRLLVQGLS
                     RVKVDGFVQESPSLRAKAVRITEPEPPKPASEEAEALLRAVKEGLEKAVSLGKAISPE
                     VMLVAAGLDDPARLADLAASNLDLKLDEAQKILETLPGLERLKAVLEILRREIRVLTV
                     QQEISGMARGEIDKNQREYFLRQQLKAIQQELGVTDDVSEEVEQYRKKLEGKNLPPEA
                     TEEVEKQLKRLERGNPDSAETATIRTFLDWITGLPWSVTSADNLDLPRARAILDEDHY
                     GLEKPKERILEFLAVRKLAPESKGPILCLVGPPGVGKTSLGRSIARALDRKFVQMSLG
                     GVRDEAEIRGHRRTYVGALPGRVIQGINQAATSNPVFILDEVDKIGADFRGDPSSALL
                     EVLDPEQNRAFRDHYLGVPYDLSKVLFITTANVLDTIQPAFLDRMEVIRLSGYTLEEK
                     LAIARRHLVPKQRKENGLSEKGISFPDDALKAIIEEYTREAGLRNLEREIGSCCRKVA
                     VRIASGKTKPVKMTPALV"
BASE COUNT         4205 a        10947 c        10424 g         4337 t
ORIGIN      
        1 tgaagaactg cccctgcgcg acgttccagg agcggatcgc gggccagtcg acctcgccgc
       61 cctggatcga cgtcgaccag ttctggttgc cgtagacgac ctgcgcgacc gagcggatcg
      121 aggccgagac gtaggaaatg gtcggcacct cgcgccggat cgcgtcgaca tcctcctccg
      181 agagcttcga gttcgagccc cagcccgacc gtgcgccgga ggaggtgacc gttccgggat
      241 ggatcatcag gtagttcgtc ccgagcgact gcagctgctg ctcggtctgg acgcgcgccc
      301 cttcgccgat cccgatcgtc gccacgacgc aggcgacgcc gatgatgatg ccgagggccg
      361 tcaggagcga tcgcatcttg ttccgcccca tcgcgcggaa cgcgaccttc aggatgctcg
      421 tcgtcttggt catctcgccc ccttcgcgga gcggcgggcc ttcaccagct cgtccgcgac
      481 gagccgtccg tcccggaacg cgacgacccg ccgggcgtac tcggcgatgt cgtgctcgtg
      541 ggtgatcagg acgatcgtct tcccctcttc gttgagggcc tggaagatcc ccatgacctc
      601 gtcggaggtc cgcgagtcga ggttcccggt cggctcgtcg ccgagcatga tcgacgggtc
      661 gttcacgagc gccctcgcga tggccacgcg ctgctgctgg cctcccgaga gctgcgccgg
      721 ggtgtggtcc cagcgatcgg cgaggccgac cctcccgagg cactcgcgcg ccttcacgag
      781 ctgctcggcg tcggagaggc ggtgcttgcg cgaatagagg agcggcagct cgacgttctc
      841 gagggccgag gtcctcgaga ggaggttgaa gttctggaag acgaagccga tcttgtcgtt
      901 ccggatcccg gcccgctcgt tccggtcgag cgtggagacg tcgaccccct cgaggacgta
      961 ggtaccggag gtcggccggt cgaggcagcc gaggatgttc atgaaggtcg acttgccgga
     1021 acccgagggc cccatgacgg cgaggaactc gcctggcgcc acgtcgagcg tcacgccgtc
     1081 gagcgcgcgg acctcggtct ctcccatctg gtagacgcgc gtcaggtcgc ggagccggat
     1141 gaccggctcg gccgagctcg ccgtcacgcc tacatccgcc ccatgccgcg cggcgcgccg
     1201 ccgccggcat tgcccgccgc ccgcgccgtc gccaggccga ggacgacctc gtccccttcc
     1261 ttcagctcgt ccgtcacgac ggccgtcagg tttccgtccg tcagcgtcgt cctcagccgc
     1321 acggtccgga gcttcccggt cgggagctcc acgtagacct cgcccgaccg agacgggccc
     1381 gggcgtcccg cgccgctccg tccggccccg ttgccgcccg gctggccccc gtcccggcgg
     1441 ccgggctgcc cggcgccggg agctccggcc gtggcgctgc cggctccgct ggccggcggc
     1501 gcgccgggcg cggcggccgc gtccaccttc ttcttcccgc cgtctgcaag gtcggccggg
     1561 tcgggcttga agcggagcgc cgcgttcggc acgcgcagga cgtcgtcccg gcggtccacc
     1621 gggacgagga cgttggccgt catcccgggc cgcagccgca gctccgggtt gccgacgtcg
     1681 agaaggaccg ggtaggtgac gacgttctgc accgtctgcg gcgagagccg gatctgcgac
     1741 acccggcccg tgaaggtccg ctcggcgaac gcgtcgacgg tgaaggtcgc ctcctggcct
     1801 tccttcacgc ggccgatgtc ggcctcgtcg atgttcgtca ggacctgcat cttcgtcagg
     1861 tcctgcgcga tcgtgaagag caccggcgcc tggaaggagg cggcgacggt ctggccgatg
     1921 tcgtactgcc ggtccacgac gacgccgtcg atcggcgaga ggatcctcgt gtaggcgagg
     1981 ttcgtctcgg cctgcttcag gctcgccgac gactgggcca ccgacgccgc ggccgagtcc
     2041 cggttcgcct tggcgttgtc gtactccgac tgcgcctgga gctgggcctc gaggagcttc
     2101 ttcgcccggg cgagcgtcag ctcggcgttc cggagctcga ccttcgcgcg ctcctggtcg
     2161 gcccggcgct ggtcgaccgt cgcctggaag ggcgtcgggt cgagctcggc gaggagctgg
     2221 ccccgccgga cctcggagtt gaagtcggcg tggagcctcg cgatgatccc cgagacctgg
     2281 ctgccgacct tgacggtggt gacggccgaa agcgtcccgg tcgcggtgac cgtcgcgacg
     2341 acggagccct tcgtcacctt ctccttccgg aacttcggcc cgctcttctt gtctttggag
     2401 aggaggagga cggccgccac cacgaccgca gcgacgatcg ccgctccgac cagtgccttc
     2461 ttcatcggtt cagcctttcg cgccgcggcc cgtcgagtct ccgaacggga atcggacgac
     2521 gaggccgagc cggaacgcgt tgaaggtccg gacggtctcc cccgtccgga ttctccgcga
     2581 ggagtactcg acgatcgcct cgattccctt ccggcccttc cagacggccg cgggctggtg
     2641 aattcgttcg ttcgacgcgg cgccgtcgta gcgccactcg ctgtagacgt agcggaaggt
     2701 cgactccggg aactccacgc cggccccgaa gaggagcgcg cgcccgtccc gctcggcccg
     2761 cggccggact tcgccgccgg ccgggtcgtc ggcccgccgc aggagcccct ggacctgaag
     2821 ggagatcgga ccgagcgtcg ccgtgacgtc gcccgagacg tcgcttcgcc ggtactcccg
     2881 tctcccgtcg gtcgggacga tcctcgcagt cgcgcccgaa aggcccggcc gcaccgtcca
     2941 gagccccctg ttgtagagga aggaggcccg gcccgagagg ccgtcgcgaa gccgtgccgc
     3001 gggatccgat tcgacgccct ttccgtcctc ttcccaggcg acccggtcgt tgcttccggc
     3061 ccagcgggcc gcccagtcga gggagtgcca gccgagcggc cggctcccga cgatctggac
     3121 gccccagtcg gggttccggt tgacaccgtt cagcgagaag agcgtcccgc cgaacgtctc
     3181 gtcggccagg ccgacgacgc tcggcacctt ccccgcgcgg agctcgccca gcggcgtccc
     3241 gacccaggcg tagccctcct cgagccacac cgggcccggg aagtagtccc ggaaccggcc
     3301 ctcgcgcgcc cgcagctccg tgcgcgcccc ccagccgtcc cgctcgagct cggccgagag
     3361 gaaggcgaag aggagctcgc cgtcccgcat ctcccgatcg cgcagcggct cgaggtcgga
     3421 gagcgcgagc ccgagaacga agcgcggcat cgtctcggcc ggcgactcgg ccggagcggg
     3481 cgacgcggcg aggaggaggg acgggagcag gacctggatg gaccggagga ctctcaagcc
     3541 tccggatgat acggcgcgcc ccgcgcggct tccggagccg cgcctgccca ggcctcggcg
     3601 tctccggaga gcccggcgaa ccgggctccg cgcccctcga ccgcgcggcg tacgcgcgtc
     3661 cggaaggcga ggacctcgag ctgtcggagc cggacggctt cgcccgtcct gcggcgctgc
     3721 tgctggcgcg cgaccgcgag gaggagcgcg ctgcggacgt actcgtgccg ctcgtccgcg
     3781 cggcccaggc gcggctcccg gcgcctccgc cacgggttgc cgaggacggg caggtcttcc
     3841 tccgggagga tcccgagccg ggcctcctcc ccgagctcgc ggcggacgcg gagcctctcg
     3901 aggacgaggg ccggaagcca ggcgaggatc gacacgaggc tggcggccgc ggccggcaga
     3961 aacgagaggc ggggcggaag gtggtccgcg agagccacga gccccgccgc gacgccggtg
     4021 gagaggaccg cgccgctcgc ccacgccgcg ccgcgtctcc agccggcctc cgccgaagcg
     4081 cgagccccct ccgcgcccca gacggagaga gccgcgaaga ccgcgcccgc tccggcggag
     4141 gcgctcccga ccccggcgcc cgcgagcgcg agcgcgcacg cggcgcccgc tccgcccgcg
     4201 atcgcgaggc tggacgccgc cgcctccgag acgaggggcg agtgcaggtc cgcgcggaga
     4261 gccgtgagga cgaggattcc gacgaggagg acgcttccga aggccagggg ggcgggaagc
     4321 gcggggagcg aagccccggc cgccgcagcg gctccggccg cgccggccag cgcggccccc
     4381 cgcggagagc gcgcgcggcg ggccagggcg agcgaagcga gaccgaggac gacgcgcggc
     4441 aggacgacgt ccacgctcag cctcgcccgc gcgagaggaa ccagagtcgc gtcgcgtcga
     4501 cgtcgcggcg gatctgcgcc gtcagctcga gcatcgacgg gaagacgagc tcgtcgcgga
     4561 gcctctcgtg gaaggagagg cgaatcggct cgccgtagac gtcggaggag aagtcgagga
     4621 tgtacgactc gatcgtcgtc gcgtagtcct cgtagacggt cggccggcgg ccgatgttcg
     4681 tgacgcagct gaacgtccgc tcgaaggagc ggagaagcac ctccgtcacg tagacgccgt
     4741 cccgcggata gagctcgttg tcggacctca ggttgatggt cgggaagccg atcttccgtc
     4801 ccatgcggtc ccccttcgcg atgacccctt ccatcgcata gggccggccg aggaggcgtc
     4861 cggcctccga caccttcccc gcctcgacga gcgtgcggac ccgggtcgac gagacggggc
     4921 cctctccgtc cgagacgacg tcgagcgtcg ccacgcggat cccggactcc tctcccagcc
     4981 gggaaaggag cgcgacgttc cccctcttcc cgcagccgaa cgcgaagccc gagccgacgt
     5041 gcgcctcgcg ggcgccgagg cagcggccca ggaggtcgcg gacgaagtct tccgcgggca
     5101 tcgccgcgaa gtccttcgtg aacgggacga cgacgaggcc gtcgacgccg agcgcggcga
     5161 tcgcctcctc gcgctggcgg agggtctgga tcagcttcgg cgccctctgc gggtgaagca
     5221 ccgtcgtcgg gtgcgggtcg aacgtcagga cgagggcggg cgcgtcgatc tcccgggcgc
     5281 gcgcgacgac ggcccggagc atcgcctggt gcccgaggtg gacgccgtcg aagttgccga
     5341 tcgtcaggac gcctccgcgc gggagatcgg acctgcgaag cgggtcgggg tagacgttca
     5401 cggccgccgc cgcctcacgg gacgacgcgc ccgacgaggt cgtagtcgtg cgccccggtc
     5461 acgcggaccc gcacgatcgc gccgggccgc gggacgaaac cctcggggac gtcgttcacg
     5521 aggaccttgc cgtcgatctc cggcgcctgc cgggcgaggc gcccctcgag gagcatctcc
     5581 gactcggtcg aggccccctc caggatcgcg tcgtggacct tcccccgaag atgccggttc
     5641 ttcctccggg agatctcgcg ctgcgcctcc atcaccgccg cgcaccgctc ccccttcacg
     5701 ggcgccggga ccgggtctcc gagcccttcc gcccccgagc cgggctcggg cgagtagggg
     5761 aagacgccga cgtggtcgag ctcggccgcc ttcacgaacg cgaggagctc ggcgaactcg
     5821 gcctcgccct ccccggggaa gccggcgatg aacgtcgagc gcagggcgat gccgggcacg
     5881 gccgcccgga gacgctcgac gagccgcagg caggactccg ggtcgccgcc gcgccgcatg
     5941 ccggcgagga cggagcggga gacgtgctgg agagggacgt cgacgtacgc gacgaaccgg
     6001 ggctcggccg ccatcagctc gaggacggag tcgtggaggg tcttcgggta ggcgtagagg
     6061 aagcggaccc acgggaagcc ggtctccctg aggagcgccg cgacgagcgt ggcgagccct
     6121 tcgcggccga ggccgaggtc ctcgccgtac cgcgtcgtgt cctgcgagat gaggacgagc
     6181 tcggagactc cctgcgcctc gagcccctgc gcctcgcgga cgagggactc gatcgaccgg
     6241 gagcgctgga ggccgcgcat ctgcgggatc gtgcagaacg tgcaggggtt gttgcaccct
     6301 tcggcgacct tcaggtaggc gtatccgcgg cggcccgtca ggacccgcgg ggccaggtcg
     6361 tcgtagagcc gcgtcgcgag gggcttcgcg gcgaaccgcg cgagcgacgg caggccgagg
     6421 gcggcggcgg gcgccttctc gagctcgtcg aggccgagga agtggtcgac ctcggggatc
     6481 tcggccgcga gctcggcccc gtacttctgg accatgcagc cggcgacgac gacccggtcg
     6541 acctcccccg cctccttgcg ccgcacctgc tcgaggacgg cgttcaccga ctcctccttg
     6601 gcctggtcga tgaagccgca ggtgttgacg atgacgacgc gggaggagtc ggggtctccg
     6661 agctccagcc cctcgcgctt caggtagccg agcatcacct cggtgtcgac gaggttcttc
     6721 gggcagccga gcgagacgat tccgacggag gggagcttca gggacatgcg tgtcgacgga
     6781 cggcgccggg gggccgccgt ccgaaccttt gattatatcc ggcggacgcg gtcccgcgcc
     6841 cggacgccgc ggggggccgt ccgctcatgc catcttcacg ccgagcgcct gagccatccg
     6901 ccgcaggtcg cccatcatcg tcgcgaagag ctcgggggtc agcgcctgag gtccgtcgca
     6961 gagcgcctcc tccgggtgcg ggtggacctc gacgatgacc ccgtcggctc cggccgccag
     7021 cgccgcgcgg gccatcggcg cgacgaacgc ggtgacgccc atggcgtgcg acgggtcgac
     7081 gatcacgggc aggtgcgtcc gcgccttcag gacctggacc gcaccgaggt cgagggtgtt
     7141 ccgcgtcgac gtctcgaacg ttcggatgcc gcgctcgcaa aggacgacgg cgcggttccc
     7201 cgcggagagg acgtattccg cggcgaggag gagctcctcg acggacgaca tcatcccgcg
     7261 cttgaggagg acgggccggc tgctctgccc gacggccttc aggagggcga agttctgcat
     7321 gttgcgagcc ccgacctgga gcatgtcggc gaactcggcg accttctcga cgtcctcggg
     7381 cgcgacgacc tccgtcacga ccggcaggtg gtactcgagc cccgcgcggc agaggaggtc
     7441 gaggccgggg atcccgagcc cctggaacga gtaggggctc gtccgcggct tgaagacgcc
     7501 gccgcgcagg acgcggcctc ccccctcccg cacgacgcgc gccgcctcga agagctgcgc
     7561 ctcgttctcc acggcgcacg gcccggcgat cacgacgaat ccctcgccga agacgacgtc
     7621 ccccaccttc accggcgtcc gggcacccgg gtcggtcgcc cgccgggccg cgagcggata
     7681 cgggaccggg ctctcgacgg ggaccggcgt cccggccggc caggacacct gcggcgcggc
     7741 gcccggcagg aggtcgacgt ccttcggcac ctcgcgagac gggtcggctt tgcggggata
     7801 acagccgagg accctgaggt agcgcgcgtt gcggccgacc ccgtcgaggg cctccgcgag
     7861 gcgggggtcg tccacgttcc cctcgacgtc gacgtagaac tggtactccc acggagtcgc
     7921 cggcatcgat cggctctcga gcttcgtcag gttcacgccc gccttcgcga gctcggcgag
     7981 gcagcgctcg agcgccccct cgcggtggtc cgtgacgagg aggagcgacg tccggcacgg
     8041 gatccgcggg tcgaatcgga acggccgccg gtggacgacg acgaaccgcg tctggttgtt
     8101 cctctggttc gcgatggacc gccggaggac gacgagcccg tgccgctccc ccgcctcctc
     8161 cgaggcgata gcggcgttct cctcgcttcc gagctccttc accttcgcga cgctgccggc
     8221 ggtgtcgaac cagctctcgg ccgtcgcgcc ggcgaggctc tcgaggaact ccgagcactg
     8281 catcagcgcc tgcgggtgcg agaagaccct ccggatccgg gcgagcggca ccttcgcgag
     8341 gccgaggaga cagtgctcca cgggccagac ctcctcgccc acgatcgaga gcttcgaggt
     8401 gccgaggagg gcgtacgtct cgttgatccc cccagccacc gagttttcga ggggcaggat
     8461 cgcgaactcg cactcttccc gctcgaccgc ctcgaccgcc tgtcggaacg accgatagcc
     8521 gacgaagatc ggctcctcgg accggtccgc gaagaacttc ttccccgcga ggtggctgta
     8581 ggccccttcc gttccctgga aggcgacccg caggagcgcc ggctcctcgt cgatcccgtt
     8641 ggcggcgcgg gtcagccggc tctcctgctg ccgcacggag gcctccacga cgtcccggaa
     8701 gatcttcacg acgtggaagg cgttcaggcc gcgccgctgc gcgtcccgac ccaccttcga
     8761 gaggaggtcg cgctcccgct ccaggtcgcg aaggaggagc gcgtgctctc ggctcttgag
     8821 cgccgcgacc tcgtcgacga ccgcggcgcg ctcggcgagg aggtcgagga gagcggcgtc
     8881 gatccggtcc agcgccgccc ggacgttcgg gaggtcgctc acgcgctcct ccgtcgccgt
     8941 gccgcgggtg tggccgccct tccgccgcgg aagggtccga aaaggcgggg cgggagcggg
     9001 acctccgggg tcatggatag atgcggtaga gcattctcgg gtacgggatg cactcgcgca
     9061 ggtggggcac gccgcacatc caggcggtga agcgctcgag gcccatcccg aagccggagt
     9121 gcgggaacga cccgtacttc ctcacgtcga ggtaccactg gaacgcctct acggggagtt
     9181 cgtgctgccg gatccgggag aggagcaggt cgtggtcgtg gatcctctgc gagcccccga
     9241 tgatctcgcc gtagccctcg ggggcgagca tgtcgaggcc gaggacgacc tccgggtcct
     9301 ccgggtcggg ctgcatgtag aacgacttga tggccgccgg atagcgcgtg accatgaccg
     9361 gcgtgtcgaa cccctcggtg agcgccgtct cctcgtcgcc gccgaggtcg tcgccgaact
     9421 tcaccgggaa gcccttcttc tcgaggatcc cgatcgcgtc gcggtagtcg atccgcggga
     9481 acggcttcgc gaccgtcgcc tcgaggaccg tcgtgtcgcg ctcgaggcgc ttcaggtcct
     9541 ccttccggcg gtcgagcacc cgcgccgtca gctccttgac gaactcctcc gcgagcgcct
     9601 tcaggccgtc gatctccagg aaggcgacct ccggctccac catccagaac tcgcgcaggt
     9661 ggcgccgggt cttcgacttc tcggcgcgga acgtcgggcc gaagcagtag accttcccga
     9721 gcgcggcggc cgccggctcg aggtagagct gccccgactg cgagaggtag gccttctcct
     9781 cctcggtgta ctgggtctcg aagaggttcg aggtcccctc gcaggcgttc ggcgtcagga
     9841 tcggcgagtc gaccctcgtg tatccgcgcg agtggtagaa gtcgtggatc gcgctctcga
     9901 cctcgctccg cacgcgcagg atcgccacct gcttcgcgga gcggagccag aggtgccgct
     9961 gcgacatcag gaagtcgacg ccgtgctcct tcggcgtgat cggccagtcg tggcagaccg
    10021 agaggacggt cagcccggtg acgccgacct cgacgccccc ctcctgccgt ggcgcctccc
    10081 ggacgacgcc cgtcacctcg aaggacgact cctgcgtcag ccggtccgac tcggtccaga
    10141 cctcctcgga gacctccttc ttcacgacga cgcactggac gtatcccgag ccgtcgcgga
    10201 agacgaggaa gcggatcttc ccgctgtccc gccggttgta gagccagccc cggagggtga
    10261 cggtctcgcc gatgcgggag cggagcccgg agacggaagc gacaggtccg gtcgacatag
    10321 caggggaatt tagcaggcgc cgcaagctcc ggcgctttgg accggaaccg gggtgaccgg
    10381 ccgccgcgcc agaggcgcgt ggtccccccg ttcagtcccg gccggctcgg agggggttcg
    10441 gggggaccgg ccgccgcgcc gcaggcgcgt ggtccccctc aggcgaagcc ggctcggagg
    10501 gggttcgggg ggaccggccg ccgcgccaga ggcgcgtggt ccccccgaga caactgtggt
    10561 ccccccgaga caaccgtgcc ccccgagaaa actaccgcct cgaacgggct gcctgcagga
    10621 ccgtcaacgc ctgcgagatg cggaggagct tccggttctt cttctggttc ccgtcgacgt
    10681 cgtcgacggg aacgacggtc ccccgcagct cgcgggccgc tttctccagc tcggcctgaa
    10741 gcgagagcag ctcgtgcggc tcgtacttcc ggagcatgac actgttcagc gtcaggtagc
    10801 cctccgtgac gtcggaggcc gtcttccagg cgggatttgc catcgcgccg acaggatgcg
    10861 ccgggcccgc cagcccgtca acggccgcgt gctagattcc cccgtccgcg cgcggcaagg
    10921 ggccgtagag agccttagga ggcgaactcg tggcatttca gggcgtcgat ttctaccagc
    10981 tcgacgggct cctcaccgag gaggagatcg ccgttcggga cctcgtccgg gacttcgtcg
    11041 acaacgaggt ccttcccgtc atcgaggact gcgcctacga ggggcgattc ccggccgagc
    11101 tcgtcccgaa gatggccgcg atgaacctgt tcggaccgac gatctccgag tacggcctcc
    11161 cgggcctgtc gaacgtcgcc tacggactga tcacgcagga gctcgagcgg ggcgactccg
    11221 ggctccgctc gttcgcgtcg gtccagtcct ccctcgtcat gttcccgatc tggaagtacg
    11281 ggtcgaagga gcagaaggac cggtggatcc ctgcgctcgc gaagggcgag gcgatcggct
    11341 gcttcggcct gacggagccc gacttcggct cgaacccagg cggaatgcgg acggtcgcga
    11401 agagggacgg cgactcctgg gtcctgaacg gcgcgaaggc ctggatcacg aacgggacga
    11461 tcgccgacgt ggcggtggtc tgggcgaagg tgggcgggcc cgagggccgc gtccacggct
    11521 tcctcgtcga gaaggggacg cccggcttca cgacctcgga gcaccggatg aagatgtcgc
    11581 tccgggcttc cgtgaccggc cagctcgcct tcgaggactg ccggatcccg gccgagaacg
    11641 tcctccccgg cgtcgacggc ctgaaggggc cgctctcctg cctcacccag gcccgctacg
    11701 ggatctcctg gggcggcatc ggctccgcca tggcgaccta cacctgcgcg ctcgactacg
    11761 cgaagtcgcg cgtccagttc atgggccggc caatcgccgc ccaccagctc gtccaggaga
    11821 agctcgcctt catgatcacg gagatcacga aggggcagct cctcgcgctc cagctcggcc
    11881 ggatgaagga tgccgggacg atgaagcccg agcacgtctc gatggcgaag cggaacaacg
    11941 tctgggtcgc gcgcgagtgc gcgaagctcg cccgcgagat cctgggcgcg aacggcatcg
    12001 tcggcgagta ccccgtcttc cggcacctcg cgaacatcga gtcggtcttc acctacgagg
    12061 ggacgcacga catccacacg ctcgtcctcg gtcaggccgt caccgggatc ccctcctaca
    12121 acccgcccgc cgaatgaagg gaaggggctc gcggacccgc ccggagacgg gccggtccgc
    12181 gagtcgcccc ggagggccgg cctccccgta gaatgcctcc cggatggcca ttaccggaaa
    12241 cctccggacg atgcagttgt cggagctgct gcagtggctc tcgatggggc agaagaccgg
    12301 tacgctggcc atccggggcg gcgcgggaga gaagcggatc tacttccaga acggccggat
    12361 catctcctcc tcctcgacga tcgagcggga gtacctcggg cacttcctcg tctcgtacgg
    12421 ctacatcacc gaggaggagc tgacgaaggc gatggaggtc caggaggaat cgaagatcct
    12481 cctcgggaag atcctcgtga tgatcggcgc gatcgccgag gaggacctcg tcgagctgat
    12541 ccgcctcaag gcctgcgaga cgatctacga catcttcctc tggcccgagg gcgccttcca
    12601 gttcctcgac ggcgagatcc cgcagcggcc gatggtgccg atcgccatcg acgtcaccgg
    12661 gatcgtcatg gagggcctcc gccgcttcga cgagtggcag aggatccggg gccggatccg
    12721 gtcgctgcgc gacgtcccca cgatcgtctc tccggtcgac gtcgaaagcc tgacggaacc
    12781 gcaccggctc atcctccggg ccatcgacgg ggtccgctcc ctcgccgaga tcgcgacggc
    12841 aacccacaac tccgacttcc ccgtcgcccg cctcgtcttc gacctgatcg agagcggaca
    12901 cgtcgagctg gcgtcggcgc caaccgtgac cgcgccgaag agctcggagc tgacccccga
    12961 ggacccctac gagaccgtct tcgaggaggc cgcgacgccg cacttcgagg gggtgtccga
    13021 cacggccgac gagctgccgc tccccccccc gccgccggag ggccggccgg cgccaccgag
    13081 agacttcgcc cggttccttc ggcggggccc cgacagcggg tcccatcgcg tgcggccctc
    13141 gggaccggtc acgacgtcgc tcgcggctcc ggtcgccgcg ccgcccgcgg caccgccgcc
    13201 gtcgacgagc ttccgcgcgg cgccgccggc cccctccccc gccggaccgc cgacctcggg
    13261 gtcgctgcgg gtcggcggcc catcggccgc atccttcctc gatatcggcg ccccgccggc
    13321 gaacgtcccc tggccggcgc cggtctcggg agcgttccgg gtcccggccc cgtccggtcc
    13381 cccttcgggg agcctgcgcg cgccggctcc ccaggggcct ccgtcctccg cctcgctccg
    13441 gtcctcggcc cctcccccga gctcgcagaa ccggctcgag atccccgcct ccgcctccgg
    13501 gagcacgttc gcgccgacgg cgattccctc cctcacgcgg ccgatggagg agctgatgtc
    13561 ctggtcgttc acgccgaacg aggcgttcat cctctcccgt atcaacgggc tctgggacgt
    13621 ccgttcgatc gcgaagatca gcccgttccc cgagggcgag gtcctccggg tcttcgagaa
    13681 gctccacgac ggcggcctga tccgctggcg ctgagcgccg caacgccacg tggagccgcc
    13741 tcttccctcc ccccttctcc tcgccgcgac ggcggcggca ttcgccgcgg cctgcgcgat
    13801 cgcgttccgc gccggtcgcc ggaccgtcgc ggccaccctg cgacaggagc tcctcgaggc
    13861 ggaacggcga ggcgaagcga tcgccgagga gggccggctg cgatccgccc ggctcgagag
    13921 ggcgaacgcg gcccaggccc gacgcgccgc cgcgctcgag agggtcctcc gcgcgacgcc
    13981 cgagctccgg gcgcacctcc cgctcgactc ggtcctgcgc aacaccgtgc aggccgcccg
    14041 gaagagcctc ggccaccggc aggtcctcct ctccctccac gaccggctcg aagggaccct
    14101 cgtgccgcgc gcccacgtcg gcctcgagga cgagtggccg tcgctcgccg aggtccgcgt
    14161 ccccgccggc cctctggcgc acgacctcgt cgccccggag acggccgagg cggcgccgct
    14221 ccggtccctc cggcggcggc tcgggctcgc tccgcccgtt gcgatgccgc tcgccgcggg
    14281 ctcgcgcgtc gtcggtctcc tggagctcgc ggagccgggg agcgacgccg tcgacgtcga
    14341 ggaggaccgc gtcgtcctcg acctcttcgc gctgcaggcc gtccaggccg tgcggctcgc
    14401 gcgcgcctac cgcaccgtcc gcctcggcac gctgcgcgac gcgctgaccg gcgtggcgaa
    14461 ccacggctcc ttccaggaga cgctccggca cgagatcgcc aggcacgagc ggagcgccga
    14521 gagcctcgtc ctcctgatgg tcgacctcga cgacttcaag gcggtcaacg accggttcgg
    14581 ccaccccgtc ggcgacgccg tcctgcgcgg cgtcgtgtcg cggctcctgg agtcggtgcg
    14641 ggagatggac gtcgtcgcgc ggtacggcgg cgaggagttc gcggtcgtcc tcccgcagac
    14701 cgacgcggcg caggggagcc gcgtcgccga gcgcctccgc gccgccgtgg ccgccgcccc
    14761 gctgccggcc gggcccgccg agccgctccc gctcaccgtc tcgatcggcc tcgccgtcta
    14821 ccccgaagac gggcggacca agggggcgct cgtcgagtgc gccgacaggg ccctctacgc
    14881 cgcgaagcgg accgggaaga accgcgtcgt ccgcttcacg cgggttccgg cggcctccag
    14941 cgacctccgc ccgagccttc ggacagctcc gcgcggcgcg taacggcgcc gcagcctccg
    15001 acggcgaacc ggagcggcca ccgcgcggcg tcgcccgcgt agtcgacgcc gacgcggggg
    15061 ccgacgagga gcgggcgcgc cgcgccgggg agcggcagga ggagctcgag ctccggccct
    15121 cccagcgatg cccccgagag ctcgcgtccg atgccgagca cccggcagag gcggccgggg
    15181 ccggaggcgg cgagaagctc gccgcgtgcg acgggcgtcc ccgtccacca ggcgagcggg
    15241 accgcggcgc gcaggaggac cgcctccggc acgcccgcct cgcgcgtcac gacgttcaga
    15301 cagtcgtgca ttccgtagac ccggaagacg taggcgtggc ccgaaggtcc ccacatcggt
    15361 gcgacgcggg gcgtccggcg gccgttccag gtgtgggcgg cgcggtcacg cacgcccagg
    15421 tacgcctcgg tctccacgac gacgaccgtc accggcggct ggcccggccg gcgtcgcacg
    15481 aggcggcagc cgaggagggc gcgcgcgacc tcttcggtcg tccgctccca gaactcgcgg
    15541 gacggtctcc cggcttgtcg gctccgcggc acgccgctat tctcgcgaac gatcatgccg
    15601 aaagagtgtc ccccgcccgt gcccggctgc ccggtcctcg tgacgggcgg cgccggcttc
    15661 atcggctctc acgtcgtcga cgcgctcctg gccctcggtc accgcgtcgt cgtcctcgac
    15721 gacctttcca ccggcgacct ccggcgcgtc cccgccgagg cgcgcttcgt ccgcggcgac
    15781 gtgcgcgacc cgggcctcga cgcgctcctc gtcgaggagc gcgtcgacac cgtcttccac
    15841 ctcgccgcgc agatcgacgt ccggaagtcg gtggaggacc ccgtcttcga cgccgaggtg
    15901 aacgtcctcg gcacgatccg cgtcgcgctc gccgccgcga aggccggggt ctcccaggtc
    15961 gtcttctcct cgagcggcgg cgcgatctac ggcgaccccg tcggcgaccg ggcggacgag
    16021 gagcaccccc tgaacccctg ctcggcctac ggcgtggcga agctcgccgg cgagaagtac
    16081 ctcgccgcgc tcgcacccga cggcggctac ctcctgaccg tcctccgcta cgcgaacgtc
    16141 tacggacccc gtcaggacgg ccgcggagag gcgggcgtcg tcgggatctt catgaaccgc
    16201 ctcctctcgg gccaggacgc cgtcatccac ggggacggcc tccagacgcg cgacttcgtc
    16261 cacgtcgccg acgtcgtcgc cgcgaaccag gcctcgttcc tccggcgcgt gggcgggacg
    16321 ttcaacgtcg gcacggggat cgagacgagc gtccgggagc tctacggact cgtcgcgtcc
    16381 gcgtgtgacg ccgaccggcc cccgcgcttc gaccccgcca agccgggcga gcagcgccgc
    16441 agcgtcctcg acgtcggacg cgcccgcaag ctcctcggcc tcgcggagtt cgtcccgctc
    16501 gagggcggga tgcacgcgac ggcggagtgg ttccggctgc accgggcggg tcagggcctc
    16561 ggcggctgat gcgccgcccc gcgccgttcg ggtcagtccg ccgaggcgga cgtggtcacg
    16621 gcgggcggct cgctcgccgg ggctccgcgc ggcttgcccg tccggcggat gacgacgacg
    16681 gtccggtcgt cttccgcggc ccgacctgag ctgaaaagcc ggacggtcga catgagcttc
    16741 tcgaccgtct ctcgcgccga gcgcttccgg tagtcgagga ggatccgccg gacccggtcg
    16801 agcccgaact cccgcccgcg gaggtcgtgc gcctcgacga tcccgtcggt gtagaggagg
    16861 aggaggtcgc ccggctccat cttcgcggcc ctccggccgt agacagcgtt cggagacggg
    16921 ccgaggacca tacccgtcga ctccatctcc gagaagctct tcgtccgggc gtgcaggtgg
    16981 atcggccccg ggtggcccgc gttgacgtag atgacctcgc cgttcgcctc gaactcggcg
    17041 tagaagagcg atacgaactt cgtcgagagc cggctcttgt tcaggatccg gttcaggcgc
    17101 tcgatcgcgc gcgcgatctt caggtcgccc ttgatcccca tccgcaggcc cacgtagacg
    17161 tcccggacga gcagcgccgc gacgagcccg tgaccggagg cgtcggcgat cgccgtgccg
    17221 aacgcttcgg ggccgagggt gatgaagtcg tagaagtcgc cgccgacggc ctcggccggc
    17281 tcggtcgcgg cccagacctc gtagtccccc atctcgggaa cgtgacgcgg caggatcgac
    17341 tgctggatgc gccgggcctc ctccaggagg tcctcgagct gctcgctctt gaccttggag
    17401 tcgaggacgg tccggatgat cccgagtgag acgagcaggt cgtcggccgc cacgcgcggg
    17461 tggacgtcga aggagaggat gaaccggtcg tcgccgaacg agaccgccgc gaaacgcgtg
    17521 acaccgaggc gagcctccag ctcccggtcg acccaggggt ccttcaggtc ggccaggacg
    17581 accccttcct cgatcgcgcg gcggatcggc tcgtacgaca ccggaacggt catcccctcc
    17641 gacaccttct tcgagcggcc gaacccgcgt cgaaggacgt actccccgtc ctcgagctcg
    17701 tagaggcggc cgccgatgat cccgagctcg ttgcggaagt tcccgacgac ggtctctccc
    17761 accgaggcga cggtggagac gacgtccgcg ctctcctcga tcgcggacag ggtcttctcg
    17821 acgttcttca ggagcgagcg gtaatccatc ggggccgcgc agtatacgtc gcccgcgcgg
    17881 ggaggttctc agccgaaccg ggcgagcgtc gcgaggatcc ggtcctggta ggcgtgcccg
    17941 cgcccctcgc cgcatccgcc gttcgcgagg atgacgaacg cgtagcgctg tccgctcctc
    18001 gcctcgatcc acccggcgag agtgacgacg ccggcgatgt tgccggtctt cgcgaagacc
    18061 ttcccgcggg tgtcgggacc gagcatccgg taacgtaggg agcccgccat gtcgccggtc
    18121 acggcgagcg tcggctcgaa cgcggcgaag tggggccagc gcgtggcgag ggcctggagg
    18181 aagtcgaggt aggcggccgc ggtcgcacgg ttctcccgcg agcgtcccga gccgtcggcg
    18241 agtcggtagc gcgccgggtc gaggccgagc gagcggagga actccgacac ctcggcgagc
    18301 ccgttctccc aggagccgag gcctcgcctc tcggcgccga gcgtcttgag gatctgctcg
    18361 gcgaagaacg actgcgagcg cttgttgctg acggcgagcg tcgggaggat cggcgtctcg
    18421 tggacgtgca ggagagggag cgacataggc gccgaccgcc gctccgtcct cacctccggc
    18481 tcgccgccga cggtgatccc ttcgtcctcg aggaccttcg tgaacgccga ggcgaggtag
    18541 agaggcgggt cgacgaccgt gacgtcgccc gaccaggtcc gtcgccgacc gaccgctccc
    18601 gacgcgacga cccgcgggct gcccgggttg cgcgagactc cgacccgcgc gccacggccc
    18661 ttgtgggtcg tcacccggcc gacgagcgac atcaggaacg ggtgcgccgg gtagaagccg
    18721 aggctcgcgc ggccgcccgg gcggaggccg cccgtgaccc tcacgaggac gacgttgtcg
    18781 ttgtaggaga gggccgagac cggagcctgc caccagtgct gctcctgggc cgccggccag
    18841 tcgggatgcg tcgtgacgtc gtcgaagaac gacgtgtcga gcgcgagtcc gtccctcacg
    18901 gcgacgatcc cgcgcgccga gagagccgca gaccaggggc ggaagaccgc ccacgggtcg
    18961 tcgtcgtaga gacggccgga gatggcggga tcgcccccgc cgcggatcac cacccggccc
    19021 gacagcgtgc cgtcgggaag gagctccccg tcctgctcga gcgtcgtgcg gaagcggtac
    19081 ccggggccga gccggtcgag ggccgcggcc gtcgtgaaga gcttcgtcgt cgacgccagg
    19141 atcagctccg tctccccgtt catcgcgagg atcggcgccc ccccgacggg ggcgatcatc
    19201 accccgacct gggaccggct cctcaccggg gtcctccgcg cgacggaaag aagccgctcc
    19261 tcgagcgtca gcggccggac cggagcggcg gcggccggcc tccgcggcgt ccgcttcgcc
    19321 gacgcggtcc ccgcatcggc ccgggccacg cagagcgcga gcccgagcag cacgaacgtc
    19381 acgccccgtc cgagaggtcg tttcgacccg gccgtcacca ccatcccgat tgtaggcgac
    19441 ctcgtcctta gaatgccgcg tgccgatccg ggtcgacctc gcctcgaaga ccgtcctcct
    19501 ttcgcccgcc gcgctcctcg ccccgacctc gcggcggatc ggcgacggcc ggggcgactc
    19561 gatggagcgc cgctggatcg gccaggccgt ccaccggcgc gtcctctcgg aggcgctcgc
    19621 gtcgatcccg ggctacgccg tcgagaaggc ggtccgccac accttcgctg tcgacggctt
    19681 caccgcggtc ctggagggac gcctcgacgg ccgcttcgag gaccccgacg gcaccgtcgt
    19741 cgtcgacgag gtgaagtcgg tccacttcgc cgaggagctg gcccgcctcg ccggctcgcc
    19801 gcggctcgac cgcttcgccc gccagctcca gtggtacctc ctcgcgatcg cgcgtgccga
    19861 ggggcggtcg gtccgcggcc ggctcgtcct cgccgacatc gagaccggcg acacccggat
    19921 cgtcccggtc gagtgggacg aggccgaggt cgaggaagac ctcgtcgccc gcgtccgcgc
    19981 ggtccttcgc gacttcttcg gcgagctcgc tctcgccgag gcgaaggcgg ccgaggccga
    20041 cgacctcgtc tttccgttcg gccgataccg ccccggccag cgcgagatgg cggccgccgt
    20101 cgcccgggcg gtccgccagg gcgagcagct cctcgtggag gcgccgaccg ggatcggcaa
    20161 gacggccgcg gcgctcttcc cgatgctcgt cgaggcgctc cgccgcggtc ggaagctcta
    20221 cgtcctcacg tcgaagacga cgcagcagga gatcttccgg aggacgctcg aggcgatctc
    20281 cgcggcctcg gcccgctccg tccgcctccg cgcgaaggag aggatgtgcg ccaacggcgt
    20341 cgtcctctgc cacgaggacc actgtgcctt cgcgaaggac tacgccgcga agctggacgc
    20401 ttcgaacctc gtggaccgcc ttctcggcgt ctcgccgcac ctcgagcccg acctcgtctt
    20461 cgaggagtcg cggtccgagc aggtctgtcc gttcgaggtc tctctcgagc tcgccgagcg
    20521 ggccgacgtc gtcatcggcg actacaacta cgccttcgac ccggtcgtcg ccctctccgg
    20581 cctgcgcgac ccggcctcgc tcgacggcgc gctcctcctc gtggacgagg tccacaacct
    20641 cgtcgagcgc ggccggggca tctggtcgcc cgaggtctcc gaggcggagg tgcggcgcct
    20701 gtcccgggcg atcggcacga cgagcccgcg gacggcggcc gccgcgcagg aggccgccga
    20761 ggaggtcctc gccctcgtcc tcgacgccgc gtccgccctc cctccgtcga ccgatgcgtg
    20821 gaacccggcc gtcgacctcg tgcccctcga cctcgaccgc ctcgacgacg tccgtctctc
    20881 gatggagtcg ctcctcgtcc ggcacctcgc cgacctccgc aacggcgccg agcgcgtccc
    20941 cgacgacccc gtgctcgggc tctacttcct ctacgccggc ttccacgacg tcgcgcggcg
    21001 agccgctcca aacggccgcc tcctcgaggc cttcgacctc ctcgcctcgc gcgggtccga
    21061 cggcgcgaag ctctcgttcc tctgcaaaga cccctcgcgc ctcctcggcg agacgttcga
    21121 cgccgccttc gccacggtcg ggatgtcggc cacgctccag ccgcccgagt tctaccgcga
    21181 ccttctgggc ctcgaccccg accggacctc cgtcctctcg ctcccctcgc cgttcccgaa
    21241 ggagaaccgg accgtcctcg tcgcccccgg agtcgacacc acctggaagc ggcgcgccgc
    21301 cgcgatgccc cgcatcgccg cgctcgtcgc ggagatcgcc gacgcctgcc ccgggaacgt
    21361 cctcgccctc ttcccgtcct atcgcttcgt cgacgacgtc cgggccctcc tcccgccgct
    21421 ccccggacac cgcgtcctcc ggccgtcgga tcgctcgacc gagctcgagc ggaacgcggt
    21481 cctctcggcg cttcgggaag aagggtcgcg catcctcctc ctcgccgtct cgggcgggcc
    21541 gttcgccgag ggggtcgact acccgggcga gatgctccac ggcgtcgtcg tcgtctcccc
    21601 cgctctcccc caggtccgct tcgagcagga gcggatgcgg ctctacttcg aggaacggtt
    21661 cgggcgcggc ttcgagtacg cctacgtcat cccggggatg acgcgcgtcg tccagagcgc
    21721 cggccgcgtc atccgctcgg agaccgacgt cggcgtcatc gccctcgtct gccggcggtt
    21781 cctccagaag ccgtaccggg cctttctccc gacgcactgg tacgacgagg cccccgacga
    21841 gctctcctcg cgcgagccgg gcgaggcggt gagggcgttc ttcgcgcgca caacgccaat
    21901 cgacgcggcc ggtccgcacg ccatcgcgga cccggatcag ggaagaggac ggctctgcgg
    21961 gcacgaggcc ccggtgcgcc gactcaggag aaggccaccc cgttccaccc cggcttcgga
    22021 tccatcgacc tgacgatgaa gcttccggcc aagcttcgcc gactcgcgtc cgccgctcgc
    22081 ctcgcccggc tccgtcggcg attcctcggg gccggctccg cggtcgatca ttgggacgag
    22141 cttgcgaggg cgggggaagc tggcgcgggg cgccaatgga cggagctggc acaggtccag
    22201 gaggtcatca acgagaggat ttccggcgag gcgcatgtca acccctatct ccacttcatg
    22261 cggagacacc ttgccgggcg ccttcccgtc gccagagcgc tcacgatcgg ctgcggcacc
    22321 ggggagctcg aaaggggact cttccgacac gggttcgcca tcgagcacga cggcttcgac
    22381 atcgcccccg aagccgtccg aggggcgatc gcggcggcga aggccgaggg ctgccgcggg
    22441 attcgctatt ccgtagccga cgcgaacgcc cttcttctcg agcctgacag ctacgacgtc
    22501 gtcttcggcg tccactccat ccaccacgtc gagaggctcg agaacgcctt cgagcagatc
    22561 gccctcgctc tgaaacccga cgggctgctc ttcctcaacg agttcgtggg cccctcgagg
    22621 ttccagtggt cgaggcggca gctcgaggtc atcgacggcg ttctacgcct cctgcccgaa
    22681 cgtttccttc gaagccgcgt tagaggcacg atcaagaagc gggcgccgcg tccgtcggtc
    22741 cgccgcatgc tcgccacgga cccgtccgaa gccgttcgct ccgacgagat tctggctctc
    22801 gtagacggct ggttcgacgt cgtcgaggta cggccctacg gcggcaccgt cctgcaccct
    22861 ctcctcgacg aaatcgccgg gaacttcgcc cgaagcgagg acggtgggag cgaaatcctc
    22921 gcggcgatct gccgtctcga gtgggcgctc atacaggcgg gcgacctcac gagcgacttc
    22981 gcagtcgtcg tcgcgaggaa gcgcggaggg gtccaggcgg gagcgcgacc tggtggacgg
    23041 acgcaagcgg cgtcgcctag actgccggac cgatacgagg tagccgagat gaatccgatg
    23101 aacgtcttca ggaggctgtc cggaaagcct gggaagcagg cggacggcac gcaggagagc
    23161 tacaaggagt ggtggaccgc gtcgtcagcg agcgagtccg gcgcctactc cacgacttac
    23221 gttgccgacg gcgaggcgga ctaccgctct cgtggctgga acggggacgc gaattcgttc
    23281 ggcgcgaggc agctcatcga gatcgccggg ctcactccct cctctcgggt cctggaggtg
    23341 ggctgcggga tggctcgcgt cgggcgcgaa atggccccga aggtggccga gtggcacggg
    23401 gccgacattt cggcgaacat gctcgagttc gctcgcgctc gaacctcgca tctcacgaac
    23461 gtgcatctgc acgagctctc ggacgtgagc ctgtcggctt tcgcggatgc gagcttcgac
    23521 ttcgtgtaca tcacgaccgt cctcatgcac ctcgacaagg aggacgtcta ccggtacctg
    23581 ctcgagtcgc accgggtcct caagccgggc gccatggcgt tcttcgacac gtggaacctg
    23641 cttcaccccg acacccaccg gatctggcgc gccatccagg cggacaacgt cggctcttcg
    23701 aagctccgag gaaggattca gttctctacg gcgcccgagc tgcggaggaa cctcgaagag
    23761 gtgggcttcg aggtggtgcg cctcgacgag gatcggctcc tgagagcgtt cgcacgaaag
    23821 cgcgaagcct acgcctacga cccatccgac acgcttccgc cgttcgggta cgtcgacgaa
    23881 cctcggaatg agagcaccgt gtccggcagc ctcgaggtcg ggggctgggt tctcgacggc
    23941 atcgaatcgg tggagatccg actcgacggc gtgcgctctc tggggagagc tcgtttgggg
    24001 ctgcctcggg cagacgtcgc ggagctgttc ccccgttatc gggacgccgc agcctgcgga
    24061 ttcggcgcgt ccgtaccaac tgaggggctg cccccgggtt cgcacactct ccaagtcatc
    24121 gcccgggacc gcgacgggtt cgagacggat ctctgtggga actaccggtc cgtaacgttc
    24181 gcgagctgag ccccccgccc gccttccgcg atcctcgcct caacgaaacg gccccgcgct
    24241 cgcgcgcggg gccgtaggtc ttcgatcggc ttcggggctc agcgggtcag ggtgttgttg
    24301 ttgacgatga agcggcgcgt cccggcggac cggtagttgc cgcggccgtc gatgacctcg
    24361 acgaggaggt cgtgctcgga gttcgcgagc ttcgtcgtgt cgaggtagaa acgccaccgc
    24421 gcgttcgtgc gctgcgggta gttcgggtag accgcggaaa cgtcggggct cgggaggccg
    24481 tactcggcgt agtcgcgacc ggtcacggcg tcgatctgcg gcacgccgtc gatcagcacc
    24541 cggacgcgga tgacgccgtc gaggtcgaga gcccagccga ggaccgggtg gatcccgccg
    24601 acgaactggt agttcatcgg gtcggagacg tagccgagcg gcgacgggtc gaggttctgc
    24661 gtcgcgcact cgatgtggac gggcagctcc ttcaggaggg tgacgttgtc ctccttgtcg
    24721 gccgccttca cctggagcat gtggttcccc tccttgaagc ccttctcggt caggaggtgg
    24781 cccacgtcga cggcgaagaa gtagccggag ttcgccgact gcgggaagcc cgggtagaag
    24841 acgttcacgt cgggccggta gaagccgtag cagtcgacga gcgcgttgtc gaggttgaac
    24901 tcgcgccggc acccgagcgt gttgaaggcg cctcgcgtgt ccttcaggac gacgccgtcc
    24961 atctcgagga agacgtgaga gaccccgccg cgctcggcgt agaccgaggt gtcgagcgcc
    25021 cagcccgaga cgaacatgac gtagcgggtg tcgcggatct cgccgccgga gggcccgccg
    25081 gggagccgga ggcagttgcc gaaccaggtc gcctggagca tcggctggtc gacgtcgccg
    25141 aagggcggga ggttcggggc gttgttgaag acctggaacc gccggatggc gaggagcccc
    25201 tgctgcccct ggtcgtcggt cgccttgacc gtgagcgtgt ggacgccgtt cgtcaggcgg
    25261 gtcgagtcga tgaagacgag gaagccggag gtcgaggcgt cggcgacgtt cgggtagaag
    25321 gccgcgacgt cgggacggtt gatgcccagg accgcctcgc gctcgaggag gccgtcgatc
    25381 aggacgtcga cgtgctcgac ccgccggtcg tcgagcgccc accccgcgac ggcgaggacg
    25441 ccgttcgcgt ggacggacga gtccgggagc gggacgtcga ggatgccgaa cggcgcctgg
    25501 ttcttcgtgt tgtcgattcg gatcgttcgc ggcgcgagga agtagcagca gccggtgacg
    25561 tccgtgatcc ggacgtggag ggtgtgcgtg ccgttggcgt agttcgccgc gcggaacgac
    25621 atctcgaagc ccggctgctc gcccggcgtc cccgcgtact gcgggagcca ctgggtcacg
    25681 tcggggcgcg ggaggttgat gttggccccg cccgcgggcg tgacccggtt cgcctcgtcc
    25741 gtcccgtcga tgaagaggtc gacgttcgag acgaggttcc cgttcagcgc gaagcccgtg
    25801 acccggacga agcccgagac gagggccccc tccgccggag cctcgatcgt cccgagggtc
    25861 tgcgccgggc agccctcttc cgggcactgc gcgaacgcgg gcgccccgag cgtcagcagg
    25921 accgccgccg tcaccggaag gaccagagcg ttgagcttca tcagtcttcc tccccaggct
    25981 cacgaggtct caggttcacg gtgtccggct ccatcgcgga atcagacccg gaactttgag
    26041 agcgtgcgtt cgtttgcggg aattctaggt gggggccaaa accctgtcaa ttcgagggtt
    26101 tccccgattt cgcgcccggc ccggccaccg gggcgaccgg accggaagct ccccgccccc
    26161 cgccgatcgg gccgccgccg cgggcgggac cctctccaag taccatgcgc ccgtgtcgaa
    26221 ggctgcctcg aacgccctcc gggtcgccct ctccctgggg ttggcggccg tcctcctctg
    26281 gctcttcttc cggagcctcg acctcgccga gctcggccgg gccctctccg acgcccgccc
    26341 ggccggactc gccgccggcc tcgcgatcac ggtcgtgaac ctcccgctgc gcgcctggcg
    26401 ctggatccgc ctgctccacc acgtcgagag ggtctccttc cgggagtcct tcgtcgccac
    26461 gacgatcggg ttcgcggcct cggtcctcct gccggcccgg gcgggcgaga tcgtgcgccc
    26521 cgccgtcctc tcccgccgga cgggacttcc cttcgctccg gccctggcct cgatcgccgt
    26581 cgagcggctg atcgacctcg tcaccgtcgt cctgctcttc gtcgtctacg ccgcgggcgg
    26641 ctgggcgccg accggtctgg ccccggaggc ccaggcgcga ctcgagctgc tgcgccgcgc
    26701 ggccctgctc gtcggcgccg gggcgctcgc cgtcttcgcg ggactcggcc tcctcgcggc
    26761 ccggcccgcg ctcgccgagc gcgtcctcgc gccgctcgaa cggcggctcc cgaagcgatt
    26821 cgccgcccgg gtcgtctccc tcctccgttc cttcctgggg gggctcgcgg ccgtccggac
    26881 cggacgcgac gtggcggtcc tggccgtctc gtcgctcgtc atgtggctcc tgaacgccct
    26941 gcagttccac gtcgtcgcgg gcgccttcgg gatccagctc gcgttcccga tctcgttctt
    27001 cgtgatgacg tgggcggtcc tcggcctcgc gatcccgacc cccggcggcg tcgggggcta
    27061 ccacgcggcg gtggcctacg cgctgacagg cttctacgcg gtcgcggccg cgcccgccgc
    27121 ggcgaccgcg ctcgccacgc acgcgctcgc gttcgtcccg atcaccgtgg ccggcgccgt
    27181 cctcgtggcc accagcggcc tcaccttccg ccgcctggcg gcgtccggct cctccggccc
    27241 ggagccctcc gggagtagtc tccgcccgtg aagtgcccgt tctgcggcgg ggtctccgac
    27301 cgcgtcgtcg attcccgcga gagccgcgag ggagaggtga tccgccgccg gcgggagtgc
    27361 ctcgcctgcc tgcgccgttt cacgtcgtac gagcgggtcg aggagtcgcc gctcgtcgtc
    27421 gtcaagagcg acggccgccg cgaggagttc gaccgcgtca aggtccttcg cggcctccgc
    27481 cgcgcctgcg agaagcgccc cgtcgaggag gcgcgcctcg aggccctctg cgacgaggtc
    27541 gaggcgctct tcccgacccg cgagccgcgc gagctgaaga cgcgcgagat cggggccttc
    27601 ctgatggaga agctgaagga ggtcgaccag gtcgccttcg tccgcttcgc ctccgtctac
    27661 cggcggttcg aggacgccgg ggacttcgtc gaggaggtcg agaccctcct ctccgacgcg
    27721 cgcgacgcct cacggaggag ccgatgacga aaggacgacc gacgacgcgc tggatctacg
    27781 gctccggcgc gggcgacccc ggcacgccgc cgccccacct ccccccggtc gacgtcgtcg
    27841 aggaggtcca cggctggcgg ctcgtcttcg agatccccgg gaccgacccg gagacggtcc
    27901 gcgtcgacgt ggcggggagg gtcgtcgtgg tccgcggcga gcggcggccg acggagcgcg
    27961 cgcacggcac gttcctctgc gtggagcgct ccgccggccc gttcgagcgc gcgatcgagc
    28021 tcccggacga gcccgacccg gacggcgcgt cggcctcgta cgccgacggc ctcctgacgc
    28081 tcgagatccc gcgccgcccg gccacccggc ggcggtcgat cccgatccgg ccggcgcggg
    28141 gcccggagaa gggctagccg atggccgaag agaccgcgag cgtcgagacg atccagatcc
    28201 cggacctcct tccggtcctg ccgctgaagg acgtcgtcct cttcccttac gtgatcgtcc
    28261 cgctctccgt ctcccgcgac cgatcgatcg ccgcggtcga cgcggcgctc tccgagcagc
    28321 ggatcctcct cctcctcgcc cagaaggacc cgtcggtcga ggagcccacg cccgccgacc
    28381 tctacgagac cgggaccgcc gccgcggtca tgcggatgct gaagctcccc gacgggagga
    28441 tccggctcct cgtccaggga ctttcccggg tgaaggtcga cggtttcgtc caggagagcc
    28501 cttccctgcg cgccaaggcg gtccggatca ccgagcccga gccgccgaag ccggcgtccg
    28561 aagaggccga ggcgctcctc agggccgtca aggaggggct ggagaaggcc gtttccctgg
    28621 ggaaggcgat ctctcccgag gtgatgctcg tcgcggcggg actcgacgac ccggcccggc
    28681 tggccgacct cgccgcctcg aacctcgacc tgaagctgga cgaggcacag aagatcctcg
    28741 agacgctccc cggcctcgag cggctgaaag cggtcctcga gatcctccgc cgcgagatcc
    28801 gcgtcctgac ggtccagcag gagatctcgg ggatggcgcg cggcgagatc gacaagaacc
    28861 agcgcgagta cttcctccgg cagcagctga aggcgatcca gcaggagctc ggcgtcaccg
    28921 acgacgtctc cgaggaggtc gagcagtacc gcaagaagct cgaggggaag aacctcccgc
    28981 ccgaggcgac cgaggaggtc gagaagcagc tgaagcggct cgagcgcggc aaccccgact
    29041 cggccgagac cgcgacgatc cggaccttcc tcgactggat caccggcctc ccctggagcg
    29101 tcacatccgc cgacaacctc gacctgcctc gcgcccgcgc gatcctcgac gaggaccact
    29161 acggcctcga gaagccgaag gagcggatcc tcgagttcct ggccgtccgg aagctggctc
    29221 ccgagagcaa ggggccgatc ctctgcctcg tcggcccgcc cggcgtcggg aagacctcgc
    29281 tcggccggtc gatcgcccgc gccctcgacc ggaagttcgt ccagatgtcg ctcggcggcg
    29341 tgcgggacga agccgagatc cgcggccacc gccgcaccta cgtcggcgcc ctcccgggcc
    29401 gcgtcatcca ggggatcaac caggccgcca cgtcgaaccc cgtcttcatc ctcgacgagg
    29461 tcgacaagat cggagccgac ttccgcggcg acccgtcctc ggccctcctc gaggtcctcg
    29521 acccggagca gaaccgcgcg ttccgcgacc actacctcgg cgtcccctac gacctctcga
    29581 aggtcctctt catcacgacc gcgaacgtcc tcgacacgat ccagcccgcg ttcctcgacc
    29641 ggatggaggt catccggctg tcgggataca cgctcgagga gaagctcgcg atcgcgcgcc
    29701 ggcacctcgt cccgaagcag aggaaggaga acggcctctc cgagaaggga atctcgttcc
    29761 ccgacgacgc gctcaaggcg atcatcgagg agtacacgcg cgaggcgggt ctccggaacc
    29821 tcgaaaggga gatcgggagc tgctgccgga aggtcgcggt gcggatcgcc tcgggcaaga
    29881 cgaagcccgt gaagatgacc ccggcgctcg tcc
//
LOCUS       DAOONL010000035        28598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1442_length_28598_cov_14.143958, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000035 DAOONL010000000
VERSION     DAOONL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_28598_cov_14.143958"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLP50_07405"
     CDS             <1..939
                     /locus_tag="PLP50_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPA51414.1"
                     /translation="KAAATHEERLAAMREMIALLPKHKGTEKLYADLKKRLAKLESEV
                     EHAAHGGRRADPGHVKREGAGQWVLLGEPNAGKSTLLAALTHAHPEIGEYPFTTRVPL
                     PGMMPFEDVQVQLVDTPAVAAGHTEPYLANLAHNADGVLLVLDVAADDGEDSAKLLLE
                     LLEKARVWPRVRPLPADAPPFLAVRPVIALGNKSDLDDGTFAEMAREAIGTDLPFHPV
                     SALSGAGLDDLRLLLWRELRRIRVYAKEPGKKPDLAAPFVLPDGATVHDLAERVHKDV
                     ASALRFARIWGHAKFDGQQVDRAHVLADRDVVELHS"
     gene            complement(950..1837)
                     /locus_tag="PLP50_07410"
     CDS             complement(950..1837)
                     /locus_tag="PLP50_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPA51415.1"
                     /translation="MGKDAKLSRPYRVESGKKFRLKDFDPGDTGGLKLEKDHAAEKLE
                     KGVDLLAALQDKLYAQDKRALLLIFQAMDAAGKDGTIKHVMSGVNPQGCQVFAFKAPS
                     AEELDHDFLWRTTKCLPERGRIGIFNRSYYEEVLVVRVHPQILAGQKLPPELVTKRIW
                     DERLEDIAAMERYLTRNGIAVVKFFLNVSKKEQRKRFLDRLEEPDKNWKFALGDVEER
                     KHWDAYQEAYEEAIRATATPWAPWYVVPADAKWYTRLVVASAVIDALERMDLAYPEVD
                     EAKKAELEKAREALATAKG"
     gene            complement(1849..2244)
                     /locus_tag="PLP50_07415"
     CDS             complement(1849..2244)
                     /locus_tag="PLP50_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51416.1"
                     /translation="MNRSRLPLALVVLALLATAYPAAADRWVLLGEKHVTDRVERDTI
                     VVTATRGDFEAIQVRVKRAAVRFVDVKVIYGNGSTDDLAIRDVIPAGGESRVIDLKGG
                     DRVLHRVDFVYEAKSLGRRGALVQVWGRR"
     gene            complement(2317..2865)
                     /locus_tag="PLP50_07420"
     CDS             complement(2317..2865)
                     /locus_tag="PLP50_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA51417.1"
                     /translation="MRPLRVVVTGSECTGKTTLARALAKRFGAPWVPEFCRGWQDAKG
                     APLEAGDVAPIARGQVAVAVAAEAAARELVVLDTDLLSTVVYARHYYGACPGWVEDAA
                     RARLADLYLLCAPDLPWTADGIRDRGKAREEMHRLFAEELARAGARVTPVSGLGPARG
                     DEAAAAVARSLARRSPGARSAG"
     gene            complement(2862..3458)
                     /gene="pnuC"
                     /locus_tag="PLP50_07425"
     CDS             complement(2862..3458)
                     /gene="pnuC"
                     /locus_tag="PLP50_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPA51418.1"
                     /translation="MNALEVAGFLTGILAVWLTAKESPWCWPTGIVNVSLYAVVFAQA
                     KLYADMGLQGVFLAVCAYGWWEWLHGGEGGGRLAVSRASRRLLAGLVLAGAVFAAALG
                     WGLHRGTDAAFPFVDSTLASFSLVAQYLQSVKKLETWWFWIAVDVGYVGMYLAKGLTL
                     TALLYAIFLALCVIGLRDWRRSLEAPPAADAAPAAEPA"
     gene            complement(3460..5676)
                     /locus_tag="PLP50_07430"
     CDS             complement(3460..5676)
                     /locus_tag="PLP50_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51419.1"
                     /translation="MTRAPKTKTRSLAGALALAPLLALAGLASAAPDDPAATTEPPAA
                     TTAATAAVVPEGDPAAENVPPMPRRTEEVVVQAVRADADTPVTKTDVPRAEIERKNLG
                     QEMPALLADLPAMTYYSDTGVGNGYAYVSMRGVGPTRINFTLDGVPLNEPEDGTLYFV
                     DFGDLGSSVESLQVQRGVGTSAAGVASFVGSVNFASLDLAEETGTTARFTLGSFGTER
                     ATVGVQTGRLGSSGFKAYVRGSYQATDGYRENSEIRQRSLFAGLSHESEKGYFKLFGF
                     LGRERSQSAYYAVEPELLEEDPRYNPLTPDDRDRFGQQFVQAQYTRFLSETTSLAGQL
                     YVNDAGGWYRLWNSSRTALREYGLDWTNLGAAVTLKTTLGPLGVTWGVHGSDFGSRRT
                     RDVVDGARSYENRGFKDEASTFVKLSRDAGDWHLYADAQLRWARFRYDGTVAVAPVSW
                     TFFNPKLGVRRDLGKDLSLYASVGMTSREPGRGDLLLGEENISVPHDLDAVKPEKVVA
                     FEVGAAWKGGPFAAQLNLYDMEFRDEIALTGEQSEVGLSIRRNAERSFRRGLEWDLAF
                     RPREELAFRFTGSWSWNRIRSWTQFYEVYDDAGEWAGTTSRTFSDVEPVLSPPFVGNL
                     IVEGSPLPGLTLGATGRYVAKAWLDNTNAEGIATPSWWKVDLSASYDLSRFVKTGSPK
                     VKVVVENLFDEEKIWANGYSWLYATRDGAGREALGAIPYYFPMATRSVTAVLDLGF"
     regulatory      complement(5757..5851)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5924..6079
                     /locus_tag="PLP50_07435"
     CDS             5924..6079
                     /locus_tag="PLP50_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPA51420.1"
                     /translation="MATGRNDPCPCGSGRKLDASCLALSRHAEEGQGYAPPSDFPARE
                     LGLARPE"
     gene            6238..6783
                     /locus_tag="PLP50_07440"
                     /pseudo
     CDS             6238..6783
                     /locus_tag="PLP50_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
     gene            6792..7916
                     /locus_tag="PLP50_07445"
     CDS             6792..7916
                     /locus_tag="PLP50_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HPA51421.1"
                     /translation="MGWGNGWGWAPYVSVASRKARAEKATAALRKKGGALEPVSIEGR
                     TIARTFWGKSWCRNLERYGDYANRLPRGRSYLANGLVVDLKVAAGRVTALVQGSHLYE
                     VEVSVARLPEPRWKEIRSECAGKIDSLVELLSGRLSEAVMAVVTRPGAGLFPAPAEIR
                     MRCSCPDSARLCKHLAAALYGIGARLDRSPELLFLLREVDHLELVSAAAETPVAAATA
                     SDDTLAGADLSSIFGIDLDGGEVQRAGATRAAPAPKRASPPALGSRPVKATARKARVQ
                     VPPAPSSPKAAGKRAGRPARKAEPTPSTGRVNEAPTRSTSRARKPGEIRASELVALGI
                     SRATIRNWLAEQVLLPTEERGVYRKTKETRRRIDAYLARR"
     gene            complement(8037..8687)
                     /locus_tag="PLP50_07450"
     CDS             complement(8037..8687)
                     /locus_tag="PLP50_07450"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPA51422.1"
                     /translation="MADTSRLPEAQRRALDRLKSVPRMADFYLAGGTAVAVHLGHRLS
                     LDLDFFSVRPDADLDAVRDSVRHAFAEASTVAQTDTAVQLLCDGAPIDLVRYPYPPLR
                     AIEQTADGVAVASLLDLAAMKLGALSRRGLRRDFWDLAEIVRRGGVSLEEACRAYRER
                     YGVAEADLYHVLRSLAWFEDAERDPMYPAGLTEAGWRDIQAFFLREAPKLLTASFG"
     gene            complement(8633..8935)
                     /locus_tag="PLP50_07455"
     CDS             complement(8633..8935)
                     /locus_tag="PLP50_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51423.1"
                     /translation="MTPVPRELRWLLWDLDAENVDVEEGAASILGRVLEHGRLSDVRL
                     VLELYGRERVHEFFRAGDHPIVSERTRSFWHAFFGADEKWPTRPDFRKHSGAPWIG"
     gene            9193..9420
                     /locus_tag="PLP50_07460"
     CDS             9193..9420
                     /locus_tag="PLP50_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HPA51424.1"
                     /translation="MRTTLHIDDDVLQAAKEIAASRSTTAGKVLSELARQALEPRSKA
                     RVRNGVPLLPRRKKGSPRPTMALVNRLRDDR"
     gene            9417..9860
                     /locus_tag="PLP50_07465"
     CDS             9417..9860
                     /locus_tag="PLP50_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPA51425.1"
                     /translation="MSRVALLDVNVLVALFDPDHVHHEAAHAWFAPSRGDGWATCPST
                     ENGVVRVLSNPAYGAEPERPGALVARLRSFCASGGHVFWPDDVTLRGARVLRAGAALS
                     HGTLTDVYLLALALAHGGRLATFDRRIPVAAVAGPEKEHLEVIPA"
     gene            complement(10140..11558)
                     /locus_tag="PLP50_07470"
     CDS             complement(10140..11558)
                     /locus_tag="PLP50_07470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HPA51426.1"
                     /translation="MTRRPVSFRSRLALVYSGILATVLGLSGAGLYFVVRAQMIRHHD
                     GELAETAAAVEEVLAQHEDCADLTPEQVQELNRYSKLVLFHSAGGQPDVFYRSPDVAS
                     VPGARELAARPELLEESPGYRMFVEGHDQVRVYSVPYRSRAGRQGVIRVMERMGDVEL
                     PLRNLRLGLLFLSPAAIALAALVTWSLARRALAPVVEVTALAREIEATQLSRRLPAPA
                     VPDEIGRLVETFNQMIARLESSFEAMKRFTADASHELRSPLANVKSTVELALGRPRSD
                     EEYRSALASVGEEAERLRRIVEDLLLLARADSGRLPFEKEEVRLDVLAREVVESFGPR
                     ALEAGLRVEAAAESEVVVLGDERWLRQLVQNLVENAVRFSPARGSGKPPALVAIGVRA
                     DGEDALLIVEDEGPGIPEAERDRIFERFYRVDASRQRGAGTGAGLGLAICSWIVDAHR
                     GRIDASGRADGGTAFRVRLPRA"
     gene            complement(11555..12229)
                     /locus_tag="PLP50_07475"
     CDS             complement(11555..12229)
                     /locus_tag="PLP50_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA51427.1"
                     /translation="MRILVVEDDPKLLESVRKGLKEAGFGADGASDGREGLQRALEDD
                     YDALVLDVMLPGLSGLDVLRELRKRRRATPVLVLSARSAVEDRVRGLDLGADDYLAKP
                     FSFAELLARLRAITRRPAVEPQTVLSAGDLTVDSVRHEARRGGQLLELTPKEFSLLEY
                     LARRKGVVLTRAMILDHVWDLDYDGGSNLVEVYVNYLRKKVDAGHEVKLIHTVRGAGY
                     VLREPS"
     gene            12878..14212
                     /locus_tag="PLP50_07480"
     CDS             12878..14212
                     /locus_tag="PLP50_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA51428.1"
                     /translation="MPVRPRGRSLALLSAALLLLPILVLHAQASASTEVDRLVAEALE
                     KNPELRAARDLASAARERVRPAGALPDPMVSLSYENDGTAFSLGEMEMTRLSLMAEQQ
                     LPFPGKLRLAEEIATKDAERAATLPDRAVLSLTGAVRRAYAGLLEARENLRLVEEQLS
                     TWKGIEEVSRARYAAGLATQQDVLRAQAERTRLAQQREADRAAEETALSEVRQLLYRP
                     ADAPVPTEARLVPGELLLVPSTRESIARALEATPELREAALAKERERLGADLARRNLK
                     PDFVASAGYMNRGSLPLMWSASVGVTVPLWAGRKQRPLIAEAERLTSAAAATEASLRR
                     LVEALTEERLVRLRRLAAEARLDAEGLLEQDRLSVEAALASYRTGSVPFVSVLEALGT
                     YFADRRSAVGRLAGLLRARADLDELSLERGSGAMPPAMPSAGPASSASPSKM"
     gene            14223..15704
                     /locus_tag="PLP50_07485"
     CDS             14223..15704
                     /locus_tag="PLP50_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51429.1"
                     /translation="MNKRTLSVALAAGLALAAGSVTLLTACSKSGSHSAHVERYQCPM
                     HPEIVKDGPGDCPICGMKLVPIEEEKPAAQAAAPSAPGERRVAFYRSPMDPNETSPTP
                     KKDAMGMDFVPVYQDELDGAAGPAGLAPVTIDATKRQLLGLRTVKVERAPFATSIRTV
                     GRVAYDERRVHHVHTRYEAYVEDVTADFTGKFVQKGEILARVYSPDLYATQKEYLLAL
                     AASRSLAGSRADGVAKGGEDLLAAARQRLLLWDIRPADIDALEKRGEPTRTLNVYAPI
                     SGFVTGRMAFHGMKVMPSDTLFDIVDLSTVWVLADVYEYELPRLSVGQKANVTLAYWP
                     GRSWSGRVTYVYPAVDEATRTVKVRLELPNPGGTLKPEMFAQVLLEGAPRQALVVPDD
                     VVIDSGQRKVVFLVEAEGRLMPREIEVGDQAEGLVEVTSGLSAGDVLARGASFLVDSE
                     SRLKAALAAMAPAPAAKPAGGSEGARAPAAVPAPPPPPAGHAH"
     gene            15716..19045
                     /locus_tag="PLP50_07490"
     CDS             15716..19045
                     /locus_tag="PLP50_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPA51430.1"
                     /translation="MSHASPGEGGVIKRIIRFSAENKYLVLSLYVIAVLIAVWVMKRT
                     PLDAIPDQSDTQVIIYSKWDRSPDIIEDQVTYPIVTALLGAPKVKTIRGSSDFGFSYV
                     YVIFEDGTDLYWARSRVLEYLSKIQGSLPAGVKTEIGSDATSVGWVFQYALVDESGTN
                     TTDELRSYQDWFLRYAVQSVPGVAEVATVGGTQRQYQVTIDPNVLSAYGVPISRVAEA
                     IRMSNEEVGGRLIELSGREYMVRGRGYLKSVKDLEQVVLKTNERGTPLTIGDVGRVSL
                     GPEIRRGIADLDGKGDVVGGIVVMRSGENALNVIERVKKKLKDIEPSLPKGVKVVTTY
                     DRSDLIQRSVENLKAKLIEEIIVVSIIILIFLWHFPSAIVPILTIPISVVLAFIPFGQ
                     MGLTINIMSLAGIAISIGVLVDGAIVEVENAYKKLQLWNDGGRPGDFHKVRLEALMEV
                     GPSVFFSLLVIAVAFLPVFTLVDQEGRLFKPLAYSKNLAMAIAAFLAITLDPAMRMLF
                     ARMDYFSFRPKWLAWVVNQITVGKYYAEEKHPVSRLLHRIYEPPCRLVLRHPKTTIAA
                     ALLAMAATVPVFLRLGSEFMPPLYEGSILYMPTTMPGISVTEADRLLQVQDRILRSFP
                     EVERVFGKAGRADSATDPAPFSMMETTVILKPESEWRPRERWYSGWAPEWLASVFRHV
                     WRDRISHEELVDEMDRALRIPGTTNAWTMPIKARIDMLSTGIRTPIGIKVFGSDLSEI
                     ERIGKEVESAVRSVPATRSAFAERVAGGYFIDFELKRDQLARYGLTVDDANMVVMTAV
                     GGEPITTVIKGRERYSVSLRYPRELREDVSRLERVLVPTMSGAQVPMGQLADIRLVQG
                     PSMIRNENGLLSGYVYVDFDTSKEDVGGFVEEAKKAVAERVTLPQGYTLVWSGQFENM
                     IRVRERLKVVIPITLVLIWLLMYMNTKSAMKTFIVLLAVPFSIIGAVWFLWILGYNTS
                     IAVWVGIIALMGLDAETGIFMLLFLDLSYDEAKARGLLRNKAELIEAIIHGAVKRVRP
                     KAMTVFAAMLGLIPIMWSVGAGSDVMKRIAAPMVGGLATSFLLELLVYPAIYMLWKQR
                     SLPKPGPDPSEPSLEGAALPA"
     gene            19085..19564
                     /locus_tag="PLP50_07495"
     CDS             19085..19564
                     /locus_tag="PLP50_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51431.1"
                     /translation="MNHASPLPRWTAALAAAVLSASALAGPSGPPKAASKTPVFKDVK
                     AQTLEFMRYADMSLAPEQEKVKDDVLSAIPTVCCKKFSMKTCCCPCNMAMTIWGLSNY
                     MLVVKGANAAELKSAVLDWVKFIGPAGYTGDACFTGGCNRPFSKNGCGGMDHKNVVF"
     gene            complement(19762..20031)
                     /locus_tag="PLP50_07500"
     CDS             complement(19762..20031)
                     /locus_tag="PLP50_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51432.1"
                     /translation="MSFAPDPRGDAERPFRGRDRDVEALGECRQRRVCEATEVRDALA
                     GGGRHLPREPGATVPREVRVHERGPEPSMHAGILVDRTVQTIEVA"
     gene            complement(20924..21868)
                     /locus_tag="PLP50_07505"
     CDS             complement(20924..21868)
                     /locus_tag="PLP50_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51433.1"
                     /translation="MRSLRLRLRAWVVVTTTVAVGAVTAAGSLEERRQIRRAESAYIG
                     AFLEHLAAMPELRSDSSTVQAHLGALSGSLARAGGKVALVALKPSDPVPLGAGAVACQ
                     RLSLSDGEWALHYWSDGRYVRAQVRRALLTRLLLAVGLVLAIVAGLEWMLRRNLLRPL
                     STISRQIERITEGGGWLAHVPDTDEELQRLTRALRALGPGLEEQTRQWIETERRVAAA
                     SVLAGVRAVTLEPLRSAQLELSLAEAALTTDAATKTRLRRAARAVHRLTETLRDAEAT
                     WFRARGGAGVSTDESRARAAKGEARALRPTASGSVEPG"
     gene            complement(21865..22536)
                     /locus_tag="PLP50_07510"
     CDS             complement(21865..22536)
                     /locus_tag="PLP50_07510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51434.1"
                     /translation="MSATEPFVPALRFHALTGLYDPLMSFLVNEATLRRRTLSLLRPE
                     PGERVLDLGCGTGSLTVRLKQEFPRVEVVGCDVDPGALGLARRKAAEAGVSIRWCLGP
                     AHDPPLPDRSVDLVTSTLLFHHLRPDGKRAALAAALRLLEPGGRLVVVDFTRPSGFLR
                     RIAFSGVRLLDGLEVTADHAAGRFAEIVESAGFERVTEADRLDTPVGTISFLTAARRR
                     EGRGG"
     gene            complement(22533..23042)
                     /locus_tag="PLP50_07515"
     CDS             complement(22533..23042)
                     /locus_tag="PLP50_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51435.1"
                     /translation="MCTATRPDALLLVEDDVSLAICVAEFLSRRGHDVELAFDLASAS
                     CALARRAWPAVVTDVDLSGPSGAEGLEVVTAAAALRPRPAIVVWSGCVSERLRREALR
                     LGADAVLEKGSLWELEAEFARQLARRQPTGAVDGADRMRDRPAAEGGSERGRPERPAE
                     AATVGGGAR"
     gene            23197..23616
                     /locus_tag="PLP50_07520"
     CDS             23197..23616
                     /locus_tag="PLP50_07520"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPA51436.1"
                     /translation="MRGRTLALTALALTLGVAPGLATAHDGHAHGMEPILGTVAHIEG
                     NRLDVADPDGKTVSVTLTSATTYFRGTTEAKRTDLKAGLRVAVEVAHGKAGLEAKVVR
                     MGASEEVVWTCPMHPEVKSAGPGKCPKCGMHLEKKKA"
     gene            23645..24262
                     /locus_tag="PLP50_07525"
     CDS             23645..24262
                     /locus_tag="PLP50_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPA51437.1"
                     /translation="MRRTTRVLLVAAALAAVWGGYTLAAAVRRGFSARDEPSGAEEVV
                     ARAFRRWATPEAIRRVPNPVAASPEVLTRARRHFADHCAGCHGNDGSGRTPIGQRLYP
                     RAPDMRGGETQRLTDGELFSIIRNGIRLTGMPAWGDGTPKEDVGTWELVHFIRHLPGL
                     TPSELAEMEALNPKSPAEFEKARQIEAFLAGSGSTALSSPERRMK"
     gene            24259..25158
                     /locus_tag="PLP50_07530"
     CDS             24259..25158
                     /locus_tag="PLP50_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51438.1"
                     /translation="MKKALGPASLLVLTLLTSPALAQMGGGGMWNGPGDMMSNGSESL
                     IVPAGVRGDGKNGAFFVTDLWIRASGTGTATIAFHPANAATADPAVTATVALSQPVTF
                     LPDLLSAQLGLTKAFGNLRISATVPIAAVARVYNQSGAGAYGLAFMGMPQRMSLTAMP
                     MAGTWDDRQFEMVMLGLLPQPGARVNATVVNTTATAVTGTLDVVDADGSAPAGAGPKS
                     WPFRIAGYSSTQVDDVLAGVASRFPSGGAGLQLRVRLDRGAGGMVMAYAVVNDNLTND
                     GYVVMGSMMNSGLGMGSGSGMGM"
     gene            25246..>28598
                     /locus_tag="PLP50_07535"
     CDS             25246..>28598
                     /locus_tag="PLP50_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPA51439.1"
                     /translation="MDIAAFQARWLGNEGGERANKDPFLNDLCDLLGVPRPDPATGDA
                     EKDLYVYEKPAVLLHAAGRPTIGKIDLYRHGRFLLEAKQGSNATRTGRTRRGTNAWHG
                     MMSDALGQAKRYVETLDAPPPFLLVVDIGHCVDLYASFDGSRVYHPFPNALANRTFFA
                     DLEELLPTLHAVWEEPLSLDPLRRTTAVTREVAAHLAGLSKELAEAGHSPEAVATFLM
                     RAIFTMFAEDVGLLPNRLFTRTLYERWIPHPETFAPDVQALWTTMNRGGSFGFEGELL
                     RFNGGLFAAPQALALTRRQLEMLHEAARCDWKNVEPSIFGTLLERALDPKERHALGAH
                     FTPRAYVERLVRATVEEPLRAEWDVVRTLVRTLVEEGSETKIAEAERAVRAFHEKLCA
                     TRVLDPACGTGNFLYVTLDLFKRLEAEVVRLLRDLRPETGASGFLDLHGVSVTPRQLL
                     GIEVKRWAKEIAELVLWIGWLQWHFRTHGDTAPDEPVLRDYGNIECRDAVLAWDRVEP
                     ETDAAGKPVTRWDGETTKPSPVTGEPIPDETARVVVERYVNPRKAEWPEAEFVVGNPP
                     FVGNKRMRTALGDGYVEALRRAHDDVPETADLVMYWWNHAASLVRAERLTRFGLITTN
                     SITQTFNRRVVQRHLVASSACHLAFAVPDHPWVDSTDGAAVRVAMTVLSQSSGAGILL
                     SLIDEEQLDDGSSRVDFRSRKGTIHADLTTGPSVSGTRALVANQDLCLQGCKLVGDGF
                     LLTSDERDNLIAVDHRAEAFLPRYVAGRDLTQTEGVRYVVDFYGLDEAQARNEFAAGY
                     QRLLHLVKPERDQNKRDTRRRNWWLFGENAPKLRRALKGLRRFIATSEVAKHRAFVFL
                     PLPGTLADGSLAGIATDDAWHLGVLSSRVHVTWAFRRGGRMGAGNDPRYQNGPCFLLF
                     PFPPSTPAQTALIRSLGEALDAHRKRQQALHPGLTITGMYNVLEKLRSGEALTAKEKV
                     IHEQGLVSVLKQIHDDLDAAVFDAYGWPSTLTDEEILERLVALNAERAEEERRGLVRW
                     LRPEFQNPGGAKAATQETLPGGDDEEEEPAAKPAAPLPWPKALPERIAAVRDLMARAP
                     SPRSLAAVTSAFAKAKPKDVEPVLESLASL"
BASE COUNT         4222 a        10348 c         9960 g         4068 t
ORIGIN      
        1 aaggcggcgg cgacgcacga ggagaggctc gcggcgatgc gcgagatgat cgcgctcctc
       61 ccgaagcaca aggggaccga gaagctctac gccgacctga agaagaggct cgcgaagctc
      121 gagagcgagg tcgagcacgc cgcgcacggc gggcggcggg cggacccggg gcacgtgaag
      181 cgcgaggggg ccggccagtg ggtcctcctc ggcgagccga acgcggggaa gtccaccctc
      241 ctcgccgccc tcacccatgc ccacccggag atcggggagt accccttcac gacccgcgtc
      301 ccgctccccg ggatgatgcc gttcgaggac gtccaggtgc agctcgtcga caccccggcg
      361 gtcgccgcag gccacaccga gccctacctc gcgaacctcg cccacaacgc cgacggcgtc
      421 ctcctcgtcc tcgacgtggc cgcggacgac ggggaggatt cggcgaagct cctcctcgag
      481 ctcctcgaga aggcgcgcgt ctggccgcgc gtgcggccgc tccccgctga tgcgccgccc
      541 ttcctcgccg tgaggccggt catcgcgctc gggaacaaga gtgacctcga cgacgggacg
      601 ttcgccgaga tggcgcgcga ggcgatcggg acggacctgc cattccatcc ggtctcggcc
      661 ctctcgggag cggggctcga cgatctacgg ctcctcctct ggcgcgagct gcggcggatc
      721 cgcgtctacg cgaaggagcc cgggaagaag cccgatctcg ccgccccgtt cgtcctcccc
      781 gacggcgcga cggtccacga cctcgcggaa cgcgtccaca aggacgtcgc ctccgccctt
      841 cgcttcgcgc ggatctgggg gcacgcgaag ttcgacgggc agcaggtcga ccgggcccac
      901 gtcctcgcgg accgcgacgt cgtcgagctc cactcctgag ccggggcgat cagcccttcg
      961 ccgtcgccaa cgcctcccgc gccttctcga gctccgcctt cttcgcctcg tcgacctccg
     1021 ggtaggcgag gtccatcctc tcgagcgcgt cgatcaccgc cgaggcgacg acgaggcgcg
     1081 tgtaccactt cgcgtcggcc ggaacgacgt accagggcgc ccagggggtc gccgtcgcgc
     1141 ggatcgcctc ctcgtaggcc tcctggtagg cgtcccagtg cttccgctcc tcgacgtcgc
     1201 cgagcgcgaa cttccagttc ttgtcgggct cctcgagccg gtcgaggaag cgtttccgct
     1261 gctccttctt cgacacgttc aggaagaact tgacgacggc gatcccgttc cgcgtcaggt
     1321 agcgctccat cgcggcgatg tcctcgaggc gctcgtccca gatcctcttc gtcacgagct
     1381 cgggcgggag cttctgcccc gcgaggatct gcgggtggac ccgcacgacg aggacctcct
     1441 cgtagtacga gcggttgaag atgccgatcc gccctcgctc cgggaggcac ttcgtcgtcc
     1501 tccagaggaa gtcgtggtcg agctcctcgg ccgacggcgc cttgaacgcg aagacctggc
     1561 acccctgggg gttcacgccc gacatgacgt gcttgatcgt gccgtccttc ccggcggcgt
     1621 ccatcgcctg gaagatgagg aggagggccc gcttgtcctg ggcgtagagc ttgtcctgga
     1681 gcgcggcgag gagatcgacc cccttctcga gcttctcggc ggcgtggtcc ttctcgagct
     1741 tgagtccgcc ggtgtcgccc gggtcgaagt ccttcaggcg gaacttcttc ccgctttcga
     1801 cgcggtaggg gcgggagagc ttcgcgtcct tgcccacgtc ggcttccttc agcgccggcc
     1861 ccagacctgg acgagcgcac cgcgccgccc gagcgacttc gcttcgtaga cgaagtcgac
     1921 gcggtggagg acgcggtcgc ctcccttcag gtcgatcacg cgggactcgc ccccggcagg
     1981 aatgacgtcg cgaatggcga ggtcgtccgt cgagccgttc ccgtagatga ccttgacgtc
     2041 gacgaagcgt acggcggcgc gtttcacccg gacctggatc gcctcgaagt cgccgcgggt
     2101 ggcggtgacg acgatcgtat ccctctcgac gcggtcggtg acgtgcttct cgccgaggag
     2161 gacccagcga tccgccgccg cggggtaggc ggtcgcgagg agggcgagga cgacgagggc
     2221 gaggggcagg cggctgcggt tcatgggtcc tccttcttcc ggctcggggc cgtgcggggt
     2281 cgatccgtgc tgccgggggc gttggttgca gggagtctat ccggcggacc gtgccccggg
     2341 ggagcgccgg gcgagggagc gcgccaccgc ggcggcggcc tcgtccccgc gcgccgggcc
     2401 gagccccgag accggggtca cgcgggcccc ggcgcgggcg agctcctccg cgaagaggcg
     2461 gtgcatctcc tcgcgggctt tgccacggtc gcggatgccg tcggcggtcc agggcaggtc
     2521 gggcgcgcag aggaggtaga ggtcggcgag gcgtgcccgc gccgcatcct cgacccagcc
     2581 ggggcaggct ccgtagtagt gacgcgcgta gacgaccgtg gagaggaggt ccgtgtcgag
     2641 gacgacgagc tcccgcgcgg ccgcctcggc ggcgacggcc accgcgacct gcccgcgcgc
     2701 gatcggcgcg acgtccccgg cctcgagcgg ggcccccttc gcgtcctgcc agccgcggca
     2761 gaactcgggg acccacgggg cgccgaatcg ctttgcgagc gcgcgcgcga gcgtcgtctt
     2821 tcccgtgcac tccgagccgg tgacgacgac gcggagcggc ctcacgcggg ctccgcggcc
     2881 ggggcggcgt ccgcggccgg cggcgcctcg agcgagcgcc tccagtcgcg caggccgatg
     2941 acgcagagcg cgaggaagat cgcgtagagg agcgccgtga gagtgagccc cttcgcgagg
     3001 tacatcccga cgtagccgac gtcgacggcg atccagaacc accaggtctc gagcttcttc
     3061 accgactgca ggtactgcgc caccaggctg aaggacgcga gcgtggagtc gacgaacggg
     3121 aaggcggcgt ccgtgccgcg gtgaaggccc cagccgagcg ccgccgcgaa gacggcgccg
     3181 gcgaggacga ggccggcgag gagccgccgc gaggcgcgcg acaccgcgag ccgcccgccc
     3241 ccttcgccgc cgtggagcca ctcccaccag ccataggcgc agacggcgag gaagaccccc
     3301 tgcagcccca tgtcggcgta gagcttcgcc tgcgcgaaga cgacggcgta gagcgagacg
     3361 ttgacgatcc ccgtcggcca gcaccacggg ctctccttcg ccgtcagcca gacggcgagg
     3421 atccccgtga ggaagccggc gacctcgagc gcgttcaggt cagaacccca ggtcgaggac
     3481 ggccgtcacg ctgcgcgtcg ccatggggaa atagtacggg atcgccccga gcgcctcccg
     3541 gcccgctccg tcgcgcgtcg cgtagagcca gctgtagccg ttcgcccaga tcttctcctc
     3601 gtcgaagagg ttctcgacga cgaccttcac cttcgggctt cccgtcttca cgaagcgcga
     3661 gaggtcgtac gaggccgaga ggtcgacctt ccaccacgac ggcgtcgcga tcccttcggc
     3721 gttcgtgttg tcgagccacg ccttcgccac gtaccgcccg gtcgccccga gcgtcaggcc
     3781 ggggaggggc gagccctcca cgatcaggtt cccgacgaac ggcggcgaga ggaccggctc
     3841 gacgtccgag aacgtccggc tcgtcgtccc ggcccactcg cccgcgtcgt cgtagacctc
     3901 gtagaactgc gtccaggagc ggatccggtt ccagctccag ctcccggtga agcggaacgc
     3961 gagctcctcg cgcgggcgga aggccaggtc ccactcgagg ccgcgccgga agctccgttc
     4021 ggcgttccgt cggatcgaga gccccacctc cgactgctcg cccgtcaggg cgatctcgtc
     4081 gcggaactcc atgtcgtaga ggttcagctg ggcggcgaag gggccgccct tccaggcggc
     4141 cccgacctcg aacgccacga ccttctccgg cttcaccgcg tcgaggtcgt gcgggacgga
     4201 gatgttctct tcgccgagga ggaggtcgcc ccggcccggc tcgcggctcg tcatcccgac
     4261 ggaggcgtag agggaaaggt ccttcccgag atcgcggcgg acgccgagct tcgggttgaa
     4321 gaaggtccag gagaccgggg cgacggcgac ggtgccgtcg tagcggaagc gggcccagcg
     4381 gagctgcgcg tcggcgtaga ggtgccagtc gccggcgtcg cgcgagagct tcacgaacgt
     4441 cgaggcctcg tccttgaagc cgcggttctc gtaagagcgc gcgccgtcga cgacgtcgcg
     4501 tgtccgccgg ctcccgaagt cgctcccgtg gacgccccac gtcaccccga gcggcccgag
     4561 cgtcgtcttc agcgtcacgg ccgcgccgag gttcgtccag tcgaggccgt actcgcggag
     4621 cgccgtccgc gacgagttcc agaggcggta ccagccgccg gcgtcgttca cgtagagctg
     4681 cccggcgagg ctcgtcgtct cggagaggaa gcgcgtgtac tgcgcctgga cgaactgctg
     4741 gccgaagcga tccctgtcgt ccggcgtgag cgggttgtag cgggggtcct cctcgaggag
     4801 ctccggctcg acggcgtagt aggccgactg cgaccgctcg cggccgagga agccgaagag
     4861 cttgaagtac cccttctcgc tctcgtggga gaggccggcg aaaaggctcc gctggcggat
     4921 ctcggagttc tcgcggtagc cgtccgtcgc ctggtaggaa ccgcggacgt aggccttgaa
     4981 gcccgacgag ccgaggcgcc ccgtctggac gcccaccgtc gcgcgctccg tcccgaagga
     5041 gccgagcgtg aagcgggcgg tcgttccggt ttcctcggcg aggtcgaggc tcgcgaagtt
     5101 gaccgagccg acgaagctcg cgacgcccgc cgccgacgtc ccgacaccgc gctgcacctg
     5161 gaggctctcg acggagctcc cgaggtcgcc gaagtcgacg aagtagagcg ttccgtcctc
     5221 cggctcgttc agcgggacgc cgtcgagcgt gaagttgatc cgggttgggc cgacgccgcg
     5281 catcgagacg taggcgtagc cgttcccgac gcccgtgtcg gagtagtagg tcatcgccgg
     5341 gaggtcggcg aggagcgcgg gcatctcctg cccgaggttc ttcctctcga tctccgcgcg
     5401 cgggacgtcg gtcttcgtga ccggcgtgtc ggcgtcggcg cggacggcct ggacgacgac
     5461 ctcctcggtg cggcggggca tcggcgggac gttctcggcg gcggggtcgc cttcggggac
     5521 gacggccgcg gtcgcggcgg tcgtcgcggc cggcggctcg gtggtcgcag ccgggtcgtc
     5581 cggggccgcg gacgccagtc cggccagcgc gaggagcggg gcgagcgcga gcgcgcccgc
     5641 gaggcttcgg gtcttcgtct tcggggctcg ggtcatggaa gcgcctcctc tcccgcgcct
     5701 gacgcgcggg cgatcgaagc ggatccgtcg gggtctctcg aggtttgcgg aagctcccgc
     5761 ttccgtttgc cgttcccttc gccggtatga cccggatcag gttcgagggt ctgctctcag
     5821 gccggtttcc cggccacccc taggcatccg tcgggcggga atatggggcg gagcgggctc
     5881 cgtgtcaacg tgcggcgcgg ctcgtcgccg catgctccac gccatggcga ccggccgaaa
     5941 cgacccctgc ccctgtggca gcgggagaaa gctcgacgcc agctgcctcg cgctttcgcg
     6001 acacgccgaa gaaggccagg gctacgctcc gccttcggat ttccctgcaa gggagctggg
     6061 gctcgcgcga cccgagtgaa agacgcgacc ccgtcccgct cgcgcgtccc gacgctcctc
     6121 gcgatcgcgg tgacgccgca cgggcatctg aagctcgaac gtctcccgtc ggacgcggac
     6181 gcccccgcga cgcccgtcgc cgcgcgcatc gaggcggcct tcgcgcgggg cgccgggagc
     6241 ggcaagctcc agcggctcgg catgctcgcc gaggagatcg cctcgcgaca ggagaaggcc
     6301 ctcgtcttca cccagttcca ggagatgacc gcgccgctcg cgtcgttcct cgcgggtccg
     6361 ttcggccggc cggggctcgt cctccacggc ggaacgccgg tggcgaagcg ccgtgcgctc
     6421 gtcgaggagt tccagcgcga ggaggggccg cccttcttcg tcgtctcggt caaggcggga
     6481 ggcacggggc tgaacctcac cgaggcctcg cacgtcgtcc atttcgatcg ctggtggaac
     6541 ccggcggtcg aggaccaggc gaccgaccgc gcgttccgga tcgggcagac gcggaacgtc
     6601 ctcgtccaca agctcgtctg ccgcggcacg gtcgaggagc ggatcgacgc cctcatcgaa
     6661 gagaagaagg ccctcgcccg ggacgtcctc ggcgagggcg cggagcagaa gatcaccgag
     6721 atgagcgacg acgagatcct ccgcctcgtc gcgctcgacc tcaccgcggc gacggtggag
     6781 taggagaaga gatgggctgg ggaaacggct ggggctgggc cccgtacgtg agcgtcgcga
     6841 gccggaaggc ccgcgccgag aaggcgaccg cggccctgcg gaagaaggga ggcgcgctgg
     6901 agccggtctc gatcgagggg cggacgatcg cgcggacttt ctggggcaag tcctggtgcc
     6961 ggaacctcga gcgctacggc gactacgcga accgtctccc gcgcgggcgc tcctacctcg
     7021 cgaacgggct cgtcgtcgac ctgaaggtcg ccgccgggcg ggtcaccgcg ctcgtccagg
     7081 ggagccacct ctacgaggtc gaggtctccg tcgcgaggct ccccgagccg cgctggaagg
     7141 agatccgctc ggagtgcgcc gggaagatcg actcgctcgt cgagctcctg agcggccggc
     7201 tgtcggaagc ggtcatggcc gtcgtcacgc gccccggcgc cgggctcttt cccgctccgg
     7261 ccgagatccg gatgcgctgc tcctgtcccg actcggcccg cctctgcaag cacctcgccg
     7321 cggccctcta cgggatcggc gcccggctcg accggagccc cgagctcctc ttcctgctcc
     7381 gggaggtcga ccatctcgag ctcgtctccg cggccgccga gacgcccgtc gccgcggcga
     7441 cggcgtccga cgacacgctc gccggcgcgg acctctcgtc gatcttcgga atcgacctgg
     7501 acggcgggga ggtgcagcgg gccggggcga cacgggccgc tccggctccg aagcgcgcga
     7561 gtccgccggc tctcggctcg cgcccggtga aggcgaccgc gcggaaggcg cgggtgcagg
     7621 tgcccccggc tccgtcgtcc ccgaaggccg ctgggaaacg ggctggtcgg cccgcgcgga
     7681 aggcggagcc gacgccgtcc acggggcgag tcaacgaagc gccgacccgc tcaacctccc
     7741 gtgcgaggaa gccgggcgag atccgcgcga gcgagctcgt cgccctcggg atctcccgcg
     7801 ccacgatccg gaactggctc gccgagcagg tcctcctgcc gacggaggag cgcggcgtct
     7861 accggaagac gaaagagacc cgccggcgga tcgacgccta cctcgcccgg cggtgacccg
     7921 tggccgggag gacggccgct tccggggcca gcgtggggcc gaaacccgga cggcgtggac
     7981 ggcgatgcgc gatcgtcgac gggaagggcg gctccgcccg ggcacgacgg aggaggtcag
     8041 ccaaagctcg ccgtgaggag cttcggtgcc tctcgaagga agaacgcctg gatgtcgcgc
     8101 cagcccgcct cggtcagccc ggccgggtac atcgggtccc gctccgcgtc ctcgaaccag
     8161 gccagtgacc ggaggacgtg gtagaggtcg gcctcggcga caccgtatct ctcgcggtag
     8221 gcccggcacg cctcctcgag cgaaacgccg cccctgcgga cgatctcggc gaggtcccag
     8281 aagtcccgcc gaagtccgcg gcgcgacagg gctccgagct tcatcgcggc gaggtccagg
     8341 agggatgcca ccgcaacacc gtcggcggtc tgctcgatcg cgcggagagg cgggtagggg
     8401 tatcgaacca ggtcgatcgg agcgccgtcg cagaggagct gcacggcggt gtccgtctgg
     8461 gccaccgtcg aggcttcggc gaaggcgtgc cggacggagt cccggaccgc gtcgaggtcg
     8521 gcgtccggcc tcacgctgaa gaagtcgagg tccagcgaca gccggtgacc caggtggacg
     8581 gcgaccgccg ttccgccagc caggtagaag tccgccatcc gcggtacgga cttcagccga
     8641 tccagggcgc gccgctgtgc ttccggaagt cgggacgtgt cggccatttc tcgtctgctc
     8701 cgaagaacgc gtgccagaag ctgcgggtcc gctcggagac gatcgggtgg tctcccgcac
     8761 ggaagaactc gtggacgcgt tcccggccgt acagctcgag cacgagccgg acgtccgaaa
     8821 ggcggccatg ctccaggacc cgcccgagga tcgacgccgc accctcttcg acgtcgacat
     8881 tttcggcatc gaggtcccaa aggagccagc gcagctcgcg ggggacgggc gtcatggcga
     8941 catcgtagcg cggcgggccc atcgtccctg ctccgagagc agggactcga cgggtaagcc
     9001 tggcggaggc cgcggggagc gatcgcggtc ggcctcgtcc gagagacgcc ctggactcgc
     9061 gccgaggcgc cggaggcgtg acgcggggcc gggctgcaca ccatggtcgg cgaagcggtg
     9121 tcatggctcc ccggaaatgt cacggtccgc gaaggagttg acatcagatg cgtgatgcgt
     9181 gatgctcgaa cggtgaggac gaccctgcac atcgacgacg acgtgctcca ggccgcgaag
     9241 gagatcgccg cgagccgctc gacgacggcg gggaaggtcc tgtcggagct cgcccgccag
     9301 gcgctcgagc cgcgctcgaa ggcacgggtc cggaacgggg tcccccttct cccgcggagg
     9361 aagaagggga gcccgcgccc gacgatggcg ctcgtcaacc gcctgcggga cgaccggtga
     9421 gccgggtcgc gctcctcgac gtcaacgtcc tcgtcgccct cttcgatccc gaccacgttc
     9481 accacgaggc cgcgcacgcc tggttcgcgc cgagccgcgg cgacggctgg gcgacctgtc
     9541 cgtcgaccga gaacggggtc gtccgggtcc tgtcgaaccc ggcctacggc gccgagccgg
     9601 agcgcccggg tgcgctcgtc gcccgcctcc ggtcgttctg cgcctcgggc gggcacgtct
     9661 tctggcccga cgacgtgacg cttcggggcg ctcgggtcct tcgcgcgggc gcggccctgt
     9721 cgcacggcac gctgacggac gtctatctcc tcgcgctcgc tctggcgcac ggcgggcggc
     9781 tcgcgacgtt cgaccggcgg attcccgtcg cggccgtcgc gggaccggag aaggagcacc
     9841 tcgaggtgat tcccgcctga gcggcccggc acccgcctgc gcccctccgc cgacctctcc
     9901 gcgcgcctcc gcgcctctca tggacatgtc cgccttccac gcccgctggc tcgggaacga
     9961 ggggggagaa cgggccagcg aggacccgtt cccgacctgc ccgcgctggc gcgatcgctt
    10021 ccgggtggag gccacgcagc gctctgacac gacgacgatc gtgccgacgt gccgctggtc
    10081 ggcggaggga gctgcccgcc ggctccctcc aactcgcatc tccgcaactc gcccgtggct
    10141 caggcgcgag gaagccgaac gcggaacgcc gtcccgccgt cggctcgccc agaggcgtcg
    10201 atccgtcctc ggtgcgcgtc cacgatccac gagcagatgg cgagcccgag gccggccccc
    10261 gttccagcgc ctcgttgccg ggaggcgtcg acgcggtaga aacgctcgaa gatccgatcc
    10321 cgctccgcct cgggaatacc gggtccctcg tcctcgacga tgagaagcgc gtcctcgccg
    10381 tcggcccgca cgccgatcgc gacgagggcc ggcggcttcc cggacccgcg tgccggcgag
    10441 aaccggacgg cgttctccac gaggttctgg acgagctgcc tcagccagcg ttcgtctccc
    10501 aggacgacca cctcggactc cgcggccgcc tcgaccctga gaccggcctc gagcgctcgc
    10561 ggcccgaacg actcgacgac ctcgcgggcg aggacgtcga gccggacctc ctccttctcg
    10621 aacgggagcc ggccggagtc ggcccgggcc aggaggagca ggtcctcgac gatccggcgg
    10681 agccgctcgg cctcctcgcc cacgctcgcg agcgcggaac ggtactcctc gtccgagcgc
    10741 ggccgtccga gcgcgagctc gacggtgctc ttcacgttcg cgagcgggct gcgaagctcg
    10801 tgcgaggcgt cggcggtgaa tcgcttcatc gcctcgaacg acgactcgag cctcgcgatc
    10861 atctggttga acgtctcgac gagacggccg atctcgtccg gcacggccgg ggcggggagc
    10921 cgccggctga gctgcgtcgc ctcgatctca cgcgcgagcg cggtcacctc gaccacgggg
    10981 gcgagcgcgc gccgggcgag cgaccacgtc acgagcgccg cgagcgcgat cgccgcgggc
    11041 gagaggaaga ggagccccag ccggaggttc ctgagcggca gctccacgtc gcccatccgt
    11101 tccatgaccc ggatcacgcc ctgccgtccc gctcgcgagc ggtacggaac ggagtagacg
    11161 cggacctggt cgtgcccttc cacgaacatc cggtagcccg ggctctcctc gaggagctcc
    11221 ggccgcgccg cgagctctct cgcgccgggg accgaggcga cgtccgggga cctgtagaag
    11281 acgtcgggct gcccgccggc cgagtggaag aggacgagct tcgagtaccg gttcagctcc
    11341 tgcacctgct cgggcgtcag gtccgcgcag tcctcgtgct gcgccagaac ctcctcgacg
    11401 gcggcggcgg tctcggcgag ctccccgtcg tggtggcgga tcatctgggc gcggacgacg
    11461 aagtagagcc cggcgccgct gaggccgagg accgtggcga ggatccccga gtagacgagg
    11521 gcgagccggg acctgaagga gaccgggcgc cgggtcacga cggctccctg aggacgtagc
    11581 cggccccgcg caccgtgtgg atgagcttca cctcgtggcc cgcgtcgacc ttcttccgga
    11641 ggtagttgac gtagacctcg acgaggttcg agccgccgtc gtaatcgagg tcccagacgt
    11701 ggtcgaggat catggcccgt gtcaggacca cccccttgcg ccgcgccagg tactcgagga
    11761 gcgagaactc cttcggggtc agctcgagga gctgcccccc ccgccgcgcc tcgtgccgaa
    11821 cggagtcgac cgtcaggtcg ccggccgaga ggacggtctg cggctcgacg gcggggcggc
    11881 gcgtgatcgc ccggagtcgc gccagcagct ccgcgaagct gaagggcttg gcgaggtagt
    11941 cgtctgcgcc gaggtcgagg ccgcggacgc ggtcctcgac ggccgagcgg gcgctcagga
    12001 cgaggacggg cgtcgcccgg cgccgcttcc ggagctcgcg aaggacgtcg agaccggaaa
    12061 gtccggggag catgacgtcg aggacgagcg cgtcgtaatc gtcctccagg gctcgctgga
    12121 ggccttcgcg cccgtccgag gccccgtcgg cgccgaaacc agcctccttc agtcccttgc
    12181 ggacgctctc gagaagcttc gggtcgtcct ccacgaccag gatcctcacc gcggtccctc
    12241 ccggcgcgag actctcacgg aatcgtccgg ctaatccgcc tctaatcttc gcgcggcatc
    12301 cttcccggcg aggaagtcat gagcgcgccc cccctctcgc cgatcgcctc cccgcctcag
    12361 ccagggcggc cggaggccgg ccggggccgg agcgtgcttc gtgcggcgct cgccctcgcg
    12421 acggccggtg tcctcacgat cctcctcgtg ccgcccgggc tcgcctcggt ctgtccggcg
    12481 gattcgccct gctgccaggg cgccgatcgt cccgcccccg gctgccccgg gtgcctgccg
    12541 tcgacggaag gacacggcca gggcgaggac ccgggttctt ctctggcccg cgtcggcggg
    12601 tgcggctgca cggtcggtcc cgctccgccg ctgcgcgccg ctctcgttgc gacggcgggg
    12661 ggcgtcgaac gcgcatcggc cggtccgccc gcggcagctt ccccgttcgt cgcctcgggc
    12721 acccccgacg gaaacgcccg aagaggaacc cgcctgccgg tgtctcccgg caagggtggg
    12781 cctctgcacc ttcggaactg cgtcctgcgg aactgatcgc tccacgtccc ttcggccgta
    12841 tcctccgccc gttcccggac cgaacacgag gtgacgaatg cccgtacgac cgcgtggccg
    12901 aagcctggcg ctcctctcgg ccgccctgct gctcctcccc atcctggtcc tccacgccca
    12961 ggcctcggcg tccaccgagg tggaccgcct cgtggcggag gccctcgaga agaaccccga
    13021 gctgcgcgcg gcgcgggacc tcgcgtcggc cgcgcgtgag cgggtccgac ccgcgggcgc
    13081 gctccccgac ccgatggtgt ccctctcgta cgagaacgac ggcacggcct tctcgctggg
    13141 cgagatggag atgacgcgtc tctccctgat ggccgagcag cagctcccgt tccccgggaa
    13201 gctccggctc gccgaggaga tcgccacgaa ggatgccgag cgggccgcca cgctcccgga
    13261 ccgggcggtc ctttcgctca cgggcgccgt ccgccgcgcc tacgccggcc tcctcgaggc
    13321 ccgagagaac ctccgcctcg tcgaggagca gctctcgacc tggaagggga tcgaggaggt
    13381 gagccgggcc cgctacgccg cgggcctcgc cacgcagcag gacgtccttc gcgcgcaggc
    13441 cgagcggacg cggctggccc agcagcgcga ggcggaccgc gcggccgagg agaccgccct
    13501 ttccgaggtc cgccagctcc tctaccgccc ggcggacgcg cccgtcccga ccgaggcacg
    13561 gctcgttccg ggtgagctgc tgctcgtccc gtcgacccgg gagtcgatcg cgcgcgcgct
    13621 cgaggcgacg ccggagcttc gagaggcggc gctcgcgaag gagcgggagc ggctgggcgc
    13681 cgacctcgcc cgccggaacc tgaagcccga tttcgtcgcc tccgccggct acatgaaccg
    13741 ggggagtctc cccctcatgt ggtccgcgag cgtcggtgtc accgtcccgc tctgggcggg
    13801 acggaagcag cgccccctca tcgccgaagc ggaacggctc acgtcggccg ccgccgcgac
    13861 cgaggcctcc ctgcgccgcc tcgtcgaggc cctgacggaa gagcgcctcg tccgcctccg
    13921 ccgcctcgcc gccgaggccc ggctcgacgc cgaaggcctc ctcgagcagg accggctctc
    13981 cgtcgaagcg gcgctcgcga gctatcgcac cggctccgtc cccttcgtct ccgtcctcga
    14041 ggctctcggc acctacttcg ccgaccgccg ctccgcggtg ggccggctcg ccggcctcct
    14101 gcgggcccgg gccgacctcg acgagctttc cctggagcgc ggctccggcg cgatgcctcc
    14161 ggccatgcct tccgccggcc cggcctcgtc ggcctctccc tcaaagatgt aggaatcgaa
    14221 agatgaacaa gcggacgctc tccgtcgccc tcgccgccgg gctcgccctg gccgccggct
    14281 ccgtcaccct gctgaccgcc tgctcgaaga gcgggtccca ttcggcccac gtcgagcgct
    14341 atcagtgccc gatgcacccg gagatcgtca aggacggccc gggcgactgc ccgatctgcg
    14401 ggatgaagct cgttccgatc gaggaggaga agccggccgc ccaggccgcg gctccctccg
    14461 cccccgggga gcggcgcgtc gcgttctacc gctcgccgat ggacccgaac gagacctccc
    14521 cgacgccgaa gaaggacgcg atggggatgg acttcgtccc ggtctaccag gacgagctcg
    14581 acggcgccgc ggggccggcc gggctcgcgc ccgtcacgat cgacgccacg aagcggcagc
    14641 tcctgggcct caggacggtg aaggtcgagc gcgccccgtt cgcgacgtcg atccgcacgg
    14701 tcggccgcgt cgcgtacgac gagcgccgcg tccaccacgt ccacacgcgc tacgaggcgt
    14761 acgtcgagga cgtcaccgcg gacttcaccg ggaagttcgt ccagaaggga gagatcctcg
    14821 cgcgggtcta tagccccgac ctctacgcga cgcagaagga gtacctcctc gccctcgcgg
    14881 cgtcgcggtc cctggccgga tctcgcgccg acggcgtggc gaagggaggg gaggacctcc
    14941 tcgccgccgc gaggcagcgg ctcctcctct gggacatccg cccggccgac atcgacgcgc
    15001 tcgagaagcg cggcgagccg acccggacgc tcaacgtcta cgccccgatc tcgggcttcg
    15061 tgacgggccg catggccttc cacggaatga aggtcatgcc ttccgacacg ctcttcgaca
    15121 tcgtcgacct ctcgaccgtc tgggtgctcg ccgacgtcta cgagtacgag ctgccgcgcc
    15181 tctccgtcgg gcagaaggcg aacgtcaccc tcgcgtactg gccggggcgc tcctggtcgg
    15241 gacgcgtcac gtacgtctac ccggccgtcg acgaggcgac ccgcaccgtc aaggtccgcc
    15301 tcgagctgcc gaacccgggc gggacgctca agccggagat gttcgcgcag gtcctcctcg
    15361 agggcgcgcc ccgccaggcc ctcgtcgtcc ccgacgacgt cgtcatcgac agcggccagc
    15421 gcaaggtcgt cttcctcgtg gaggcggagg gccgcctgat gccgcgcgag atcgaggtcg
    15481 gcgaccaggc cgagggcctc gtcgaggtca cgtcgggcct ttcggccggc gacgtcctcg
    15541 cgcgcggcgc gagcttcctc gtcgactccg agtcgcgcct gaaggcagcc ctcgcggcga
    15601 tggcgcctgc gcccgccgcg aagcccgccg gcggctcgga aggcgcccgc gctccggcag
    15661 ccgtcccggc ccccccgccg ccccccgccg gccacgcgca ctgaaggagg ccgcgatgag
    15721 tcacgcaagc cccggagaag gcggcgtcat caagcggatc atccgctttt cggccgagaa
    15781 caagtacctc gtcctctccc tgtacgtcat cgcggtcctg atcgccgtct gggtcatgaa
    15841 gcggacaccg ctcgacgcga ttccggacca gtccgacact caggtcatca tctattcgaa
    15901 gtgggaccgg agccccgaca tcatcgagga ccaggtcacc tacccgatcg tcaccgcgct
    15961 cctgggcgcg ccgaaggtga agacgatccg gggctcctcc gacttcggct tctcctacgt
    16021 ctacgtgatc ttcgaggacg gcacggacct ctactgggcc cgctcccgcg tcctggagta
    16081 cctctccaag atccagggga gcctgccggc cggcgtcaag accgagatcg gctccgacgc
    16141 cacgagcgtc gggtgggtct tccagtacgc cctcgtcgac gagtcgggga cgaacaccac
    16201 cgacgagctc cggtcgtacc aggactggtt tctcagatac gcggtgcagt cggtgccggg
    16261 cgtcgcggaa gtggccacgg tcggtgggac gcaacggcaa taccaggtca cgatcgaccc
    16321 gaacgtcctc tccgcctacg gcgtcccgat ctcgcgggtc gccgaggcga tccggatgag
    16381 caacgaggag gtgggcgggc ggctcatcga gctctccggc cgggagtaca tggtccgcgg
    16441 ccgcgggtac ctgaagagcg tgaaggacct cgagcaggtc gtcctgaaga cgaacgaacg
    16501 cgggacgccc ctcacgatcg gagacgtcgg acgggtctcg ctcggccccg agatccggcg
    16561 cgggatcgcg gacctcgacg gcaaggggga cgtcgtcggc ggcatcgtcg tgatgcggag
    16621 cggcgagaac gccctcaacg tcatcgagcg ggtgaagaag aagctgaagg acatcgagcc
    16681 gtccctgccg aagggcgtca aggtcgtcac gacctacgac cgctccgacc tgatccagcg
    16741 ctctgtcgag aacctgaagg ccaagctgat cgaggagatc atcgtcgtct cgatcatcat
    16801 cctcatcttc ctctggcact tcccgagcgc gatcgtcccc atcctcacga tcccgatctc
    16861 ggtcgtcctc gccttcatcc cgttcgggca gatggggctg acgatcaaca tcatgtcgct
    16921 cgcgggcatc gcgatctcga tcggcgtcct cgtcgacggg gcgatcgtcg aggtcgagaa
    16981 cgcctacaag aagctccagc tctggaacga cggggggagg cctggagact tccacaaggt
    17041 ccggctcgag gccctgatgg aggtcgggcc gtccgtcttc ttctcgctgc tcgtcatcgc
    17101 ggtcgccttc ctgcccgtct tcacgctcgt cgaccaggaa gggcgactct tcaagccgct
    17161 ggcctattcg aagaacctcg cgatggcgat cgcggcgttc ctcgccatca cgctcgaccc
    17221 cgcgatgagg atgctgttcg cccggatgga ctacttcagc ttccgcccga agtggctcgc
    17281 ctgggtcgtc aaccagatca cggtcgggaa gtactacgcg gaggagaagc atcccgtcag
    17341 ccgcctcctc caccggatct acgagccccc ctgccgcctc gtcctcaggc acccgaagac
    17401 gacgatcgcg gccgccctcc tcgcgatggc ggcgacggtt ccggtcttcc tgaggctcgg
    17461 ctcggagttc atgccgcccc tctacgaggg gagcatcctc tacatgccga cgaccatgcc
    17521 cggcatctcg gtgaccgagg ccgaccgcct tctccaggtg caggaccgga tcctgaggtc
    17581 gttcccggag gtcgagcgcg tcttcgggaa ggccggccgc gccgacagcg cgaccgaccc
    17641 ggccccgttc tcgatgatgg agacgaccgt catcctcaag ccagagagcg agtggcgccc
    17701 ccgcgaacgc tggtacagcg gatgggcgcc tgagtggctc gcgtcggtct tccggcacgt
    17761 ctggcgggac cggatctccc acgaggagct cgtggacgag atggaccggg cgctgaggat
    17821 cccggggacg acgaatgcct ggacgatgcc gatcaaagcc cgcatcgaca tgctctcgac
    17881 ggggatccgg acgccgatcg ggatcaaggt cttcggctcg gacctctcgg agatcgagcg
    17941 gatcggcaag gaggtcgagt ccgccgtgag gagcgtcccg gccacgcgat cggcattcgc
    18001 cgagcgggtc gcgggcggct acttcatcga cttcgagctc aagcgcgacc agctcgcccg
    18061 gtacggactg acggtcgacg acgcgaacat ggtcgtcatg acggcggtgg gcggcgagcc
    18121 gatcacgacc gtcatcaagg ggcgcgagcg atactccgtc tccctccgct acccccgcga
    18181 gctccgtgag gacgtctcgc gtctcgagcg ggtcctcgtc ccgacgatgt cgggagccca
    18241 ggtcccgatg ggccagctcg ccgacatccg gctcgtccag ggcccctcga tgatccggaa
    18301 cgagaacggg ctcctctcgg gctacgtcta cgtcgacttc gacacctcga aggaggacgt
    18361 cggcggcttc gtcgaggaag cgaagaaggc ggtcgcggag cgggtcacgc tcccgcaggg
    18421 gtacacgctc gtctggagcg gccagttcga gaacatgatc cgcgtgcggg aacgtctgaa
    18481 ggtcgtcatc ccgatcacgc tcgtcctgat ctggctcctg atgtacatga acacgaagtc
    18541 ggcgatgaag acgttcatcg tcctcctcgc cgtccccttc tcgatcatcg gcgcggtctg
    18601 gttcctctgg atcctcggct acaacacgtc gatcgccgtc tgggtcggga tcatcgcgct
    18661 catgggcctc gacgccgaga cgggcatctt catgctgctc ttcctcgacc tctcctacga
    18721 cgaggcgaag gccagaggtc tcctgcggaa caaggccgag ctgatcgagg cgatcatcca
    18781 cggcgccgtg aagcgcgtcc gcccgaaggc gatgaccgtc ttcgccgcga tgctcgggct
    18841 catcccgatc atgtggtcgg tcggggccgg ctccgacgtc atgaagcgga tcgccgcccc
    18901 gatggtgggc ggcctcgcga cgtccttcct cctcgagctc ctcgtctatc ccgccatcta
    18961 catgctctgg aagcagcgaa gcctcccgaa acccgggccc gatccctcgg agccgagcct
    19021 cgagggcgcg gcgcttcccg cctgacaccg atcgaaccga actgacggaa ccaggagcta
    19081 cgacatgaac cacgcctccc ccctccctcg ctggaccgcc gccctcgcgg ccgccgtcct
    19141 ctccgcttcg gccctggccg gcccttccgg cccgcccaag gccgcctcga agacgcccgt
    19201 cttcaaggac gtgaaggccc agacgctcga gttcatgcgg tacgccgaca tgagcctcgc
    19261 gccggagcag gagaaggtca aagacgacgt cctctccgcc atcccgacgg tctgctgcaa
    19321 gaagttctcg atgaagacct gctgctgccc gtgcaacatg gcgatgacga tctggggcct
    19381 ctcgaactac atgctcgtcg tgaagggggc gaacgccgcc gagctgaagt cggcggtcct
    19441 cgactgggtg aagttcatcg ggccggccgg ctacacgggt gacgcctgct tcacgggcgg
    19501 ctgcaaccgc ccgttctcga agaacggctg cggcggcatg gaccacaaga acgtcgtctt
    19561 ctgaggaggc gaacgatgcg caccccggcg atcctcctct tcgtcctgct ggtcaacgcc
    19621 tcggcaccgg cacaggagca cgttcacggc gagccggggc cggcaccgcg gaccgagccc
    19681 ccgtccgcct ccgcccgcgg cgctcgggct ctgctccgcg atgaggcgtc cgcactgcgg
    19741 gccgttcggg cagccgcccg ctcacgcgac ctcgatcgtc tgaaccgtgc ggtcgacgag
    19801 tatgccggcg tgcatcgagg gctccgggcc gcgctcgtgg acgcggactt cacgggggac
    19861 ggtggctccg ggttcgcggg gaaggtgtcg gccgccgccc gcgagagcgt cgcgaacctc
    19921 cgtggcctcg cagacgcgtc gctgccggca ttcgccgaga gcttcgacgt cgcgatcgcg
    19981 gccgcggaac ggacgctcgg cgtcgcctct ggggtcgggg gcgaaggaca cgatgacgcc
    20041 gcgccaccga gacgaggcgg cgggtgcggc tccggacggt ccggacacgg agagcactaa
    20101 cgggcagccc gggctctgcc cccgcctcgg ggtcgcgccg agctcggcaa agcagtcacc
    20161 cccgaggcgg cggcggcgtc cccgtccctt cgcagagcct cgaggtctcc gagggggagc
    20221 ggacgccgcc agccccccgc tttcgtcgcg cccggctcga cttcgcgcgg ccctccgggc
    20281 ggcccggcga acgccggcgg aacagccgcg accctgaagg acggggtgat ggacgaggaa
    20341 gagacgctcg aggacggccg cacgcctcgg cgccgctgag gcccgcggcg tcgccggggc
    20401 cccctcgtac acctcacccg agcgggcctg aggcgccgtg gccgagaccg ccttcgacgg
    20461 cttctcgctc gggattctgc tgtgcgagat cctcaggggc cggagcccct ttccggcacg
    20521 cgacggcaac agcgtcagga ggatgggcca ggagctcggc cgggagaagc ccttccgccc
    20581 caaccgagcc gccgggcaag ccctctcgac cgtcagtcgc gggatccagg cgaagcggcc
    20641 ccccggcccg gtcttccgcc gcccgggccc tcgccgccga gattcgccgc cgtcgcgtgc
    20701 tacgccccgt gaatgccgtt cgtccgtcgg ccggagagcg tctcacgacc tgggctcgcc
    20761 gccgccctgt ggcctcctcc gtagacgtca gcctggtcgg tgccgctctc gtcgaggccg
    20821 tcgtcacgat gacacgtgtc gccgcgacgt agcctcgctg atgcggaagg tcggcctcgc
    20881 ggaagcgggg ctcgccgggc ggcgctggca aggggagcgg gcctcagccg ggctccacgc
    20941 tccctgaggc cgtcgggcga agagcacggg cctccccttt cgccgctcgg gcacggctct
    21001 cgtccgtcga gacgccggcg ccgccacgag cgcggaacca ggtggcctcc gcgtcgcgca
    21061 gagtctcggt caaccggtgg acggcacggg cagcgcgtcg cagccgcgtc ttcgtcgcgg
    21121 cgtcagtggt gagagccgcc tccgccaggc tcagctcgag ctgcgcggat cgaagcggct
    21181 cgagggtcac cgcccggact cccgcgagga ccgacgccgc ggcgacgcgt cgctcggtct
    21241 cgatccactg gcgagtctgc tcctcgagcc cgggtccgag cgcccgaagc gctcgtgtca
    21301 gccgctggag ctcctcatcc gtgtcgggaa cgtgcgcgag ccagcctccg ccttccgtga
    21361 tccgctcgat ctggcgcgag atcgtcgaga gcggtcggag gaggtttcga cggagcatcc
    21421 attcgagccc tgcgacgatg gcgaggacga ggccgacggc gagcaggagc cgcgtgagca
    21481 acgcccgccg gacctgcgcc cggacgtatc ggccgtcgct ccagtagtgc agcgcccact
    21541 cgccgtccga gagggaaaga cgctggcagg cgacggcccc ggcgccgagc gggaccggat
    21601 cggaaggctt caaggctacg agcgcgacct ttcccccggc cctcgcgagg gagcccgaca
    21661 gggcaccgag gtgcgcttgc accgtggaag agtcgctccg aagctccggc atcgcagcca
    21721 ggtgctcgag gaacgcaccg atgtacgccg actcggcgcg tcggatctgc cgtcgctcct
    21781 cgaggcttcc cgccgccgtc accgctccga cggcgacggt cgtcgtcacc acgacccagg
    21841 cgcggaggcg gaggcggagg ctcctcatcc gccgcgaccc tcgcggcggc gggccgcggt
    21901 gaggaaggag atcgtgccga cgggcgtgtc gaggcgatcg gcctccgtca cgcgttcgaa
    21961 cccggcggac tccacgatct ccgcgaaccg ccccgccgcg tggtccgccg tgacctcgag
    22021 accgtcgagg agccggactc cggagaaggc gatccgacga aggaaacccg atggcctcgt
    22081 gaagtcgacg acgacgagcc gtccgcccgg ctcgagcagc ctcagggcgg cggccagcgc
    22141 agcgcgcttg ccgtccggcc ggaggtggtg gaagagcagc gtcgaggtga cgagatccac
    22201 cgatcggtcc gggagcggcg ggtcgtgggc gggcccgagg caccaccgta tcgagacccc
    22261 ggcctcggcg gccttccgcc gggccagacc gagcgctccc gggtccacgt cgcaaccgac
    22321 cacttcgacc cgcgggaact cctgcttgag ccggaccgtg agggagcccg tgccgcaccc
    22381 caggtcgagg accctctctc cgggctccgg ccggaggagg ctcaacgtgc ggcgccggag
    22441 cgtcgcctcg ttcacgagaa aagacatcag ggggtcgtag agaccggtga gggcatggaa
    22501 cctcagcgcc gggacgaacg gctcggtcgc gctcaccgcg cgcctccccc caccgtggcc
    22561 gcctccgccg ggcgttccgg tctcccgcgt tcgctgcctc cttccgcggc gggtcggtcc
    22621 cgcatccggt ccgctccatc aaccgccccg gtcggctgcc gtctcgcgag ctgacgagcg
    22681 aactccgcct cgagctccca caggctcccc ttctccagga cggcgtccgc gccgagccgg
    22741 agcgcctcgc gccggaggcg ctcgctcacg cacccgctcc agacgacgat cgcggggcgc
    22801 ggccgcagcg ccgccgcagc cgtcacgacc tccaggcctt ccgcgccact cgggcccgag
    22861 aggtcgacgt cggtgacgac ggccggccag gcccgccgag cgagggcgca cgacgcggag
    22921 gcgagatcga aagcgagctc gacgtcgtgt ccgcgtctcg acaggaactc cgcgacacag
    22981 atggcgagcg agacgtcgtc ctcgaccagg agcagagcat ccggacgggt cgccgtgcac
    23041 atggcccgag gatgggaggc gaaccttgga aaccgatgag agacggtgct tcgggccgct
    23101 ctaatcgctc tctaaggtcg gagccgtatt tttccggcca cgagaggggc cgcgggcgcc
    23161 gccggcggcc gacgcccctc gacaggagga ttcgacatgc gcggaaggac ccttgccctc
    23221 acggccctcg ctctcacgct cggcgtcgcg ccgggtctcg ccacggcgca cgacggccac
    23281 gctcacggga tggagccgat cctcggcacg gtggcgcaca tcgaggggaa tcgcctcgac
    23341 gtcgccgacc cggacgggaa gacggtgtcg gtcacgctca cctccgcgac cacctacttc
    23401 cgtgggacga cggaggcgaa gaggaccgac ctgaaggccg ggctgcgcgt ggccgtcgag
    23461 gtcgcccacg ggaaggcggg gctcgaagcg aaggtggtcc ggatgggagc gtcggaggag
    23521 gtcgtctgga cctgcccgat gcacccggaa gtgaagagcg cgggcccggg caagtgcccg
    23581 aagtgcggca tgcacctcga gaagaagaag gcgtgatgac gaggcggacg gcgggagggg
    23641 acccgtgagg cggacgacac gagtcctcct ggtcgcggcg gccctcgctg ccgtgtgggg
    23701 cggatacacg ctcgccgccg ccgtccgtcg cggcttcagc gcgcgagacg agccgtccgg
    23761 ggccgaggag gtcgtggctc gggctttccg gcggtgggcg accccggagg cgatacgccg
    23821 tgttccgaac cccgtcgccg cgagccccga ggtgctcacc cgggcgcgcc gccacttcgc
    23881 cgaccactgc gccggctgcc acggaaacga cgggagcggc cggacgccga tcggccagcg
    23941 cctctatccg cgcgcgcccg acatgcgcgg cggcgagacc cagcgcctca ccgacggaga
    24001 gctcttctcg atcatccgga acggaatccg cctcaccggc atgcccgcct ggggcgacgg
    24061 gacgcccaag gaagacgtcg gcacgtggga gctcgttcac ttcatccgcc accttcccgg
    24121 gctgacgccc tcggagctcg cggagatgga agcgctgaac ccgaagagcc ccgccgagtt
    24181 cgagaaggcc cgccagatcg aggcgttcct cgccggctcc ggctcgacgg ctctgtcctc
    24241 gcctgaaagg agaatgaaat gaagaaggcg ctcggcccgg ccagcctcct cgtcctgacg
    24301 ctgctcacgt cccccgccct cgcgcagatg ggcggaggtg ggatgtggaa cggccccggc
    24361 gacatgatgt cgaatggctc cgaatccctc atcgtcccgg ccggagtccg aggcgacggg
    24421 aagaacggcg ccttcttcgt caccgacctc tggatccgcg cgagcggcac cggcacggcg
    24481 acgatcgcgt tccacccggc gaacgccgcc accgccgatc cggccgtgac ggccactgtg
    24541 gcgctctcgc agcccgtgac cttcctcccg gacctcctct ccgcgcagct cggcctgacg
    24601 aaagcgttcg ggaacctccg gatcagcgcc acggtcccga tcgccgcggt ggcccgcgtc
    24661 tacaaccaat cgggggccgg agcgtacggt ctcgcgttca tggggatgcc ccagcgcatg
    24721 tcgctcacgg cgatgccgat ggcgggcacc tgggacgacc gccagttcga gatggtcatg
    24781 ctcggcctcc tgcctcagcc cggcgcccgc gtgaacgcca ccgtcgtgaa cacgaccgcg
    24841 acggccgtga ccgggacgct ggacgtcgtc gacgccgacg gcagcgctcc cgccggagcg
    24901 ggaccgaaga gctggccctt ccggatcgcc ggctactcgt cgacgcaggt ggacgacgtc
    24961 ctcgctggcg tcgcctcgcg cttcccgtcg ggcggtgccg gcctgcagct ccgggtccgc
    25021 ctcgaccgcg gcgcgggcgg gatggtcatg gcctacgccg tcgtgaacga caacctgacg
    25081 aacgacggct acgtcgtgat ggggagcatg atgaactcgg gcctggggat gggatcgggc
    25141 tccggtatgg gaatgtaggc gtcgagaccg tcccccggga cgtggctttg cggggcgccg
    25201 gccggcgccc ctctgctaac ctccccccgc gcctccgcgc ccttcatgga catcgccgcc
    25261 ttccaggccc gctggctcgg gaacgagggg ggagaacggg ccaacaaaga cccgttcctg
    25321 aacgacctct gcgacctcct cggcgtgccc cgtcccgacc cggcgacggg cgacgcggag
    25381 aaggacctct acgtctacga gaagccggcc gtcctccttc acgcggccgg caggccgacg
    25441 atcgggaaga tcgacctcta ccggcacgga cgcttcctgc tcgaggccaa gcagggctcg
    25501 aacgcgaccc ggacgggccg gacgcggcgc ggaacgaacg cctggcacgg gatgatgtcc
    25561 gacgccctcg ggcaggcgaa gcgctacgtc gagacgctcg acgccccgcc cccgttcctc
    25621 ctcgtcgtcg acatcggcca ctgcgtcgac ctctacgcct ccttcgacgg ctcgcgcgtc
    25681 taccacccgt tcccgaacgc cctcgcgaac cgcaccttct tcgccgacct cgaggagctc
    25741 ctcccgacgc tccacgccgt ctgggaggag ccgctctccc tcgacccgct ccggcggacg
    25801 accgccgtca cgcgcgaggt cgcggcgcac ctcgccggcc tctcgaagga gctggccgag
    25861 gcggggcatt ccccggaggc ggtcgcgacg ttcctgatgc gggccatctt cacgatgttc
    25921 gccgaggacg tcggcctcct cccgaaccgc ctcttcacga ggaccctcta cgagcgctgg
    25981 atcccgcacc cggagacgtt cgcgcccgac gtccaggcgc tctggacgac gatgaaccgg
    26041 ggcgggtcgt tcggcttcga gggggagctc ctccgcttca acggcggcct cttcgcggcg
    26101 ccgcaggccc tcgccctcac gcgccgccag ctcgagatgc ttcacgaggc ggcccgctgc
    26161 gactggaaga acgtcgagcc ctccatcttc gggacgctcc tcgaaagggc cctcgacccg
    26221 aaggagcgcc acgccctcgg cgcccacttc acgccgcgcg cctacgtcga gcggctcgtg
    26281 agggcgaccg tcgaggagcc gctccgcgcc gagtgggacg tcgtgcggac gctcgtgagg
    26341 accctcgtcg aggagggctc ggagacgaag atcgccgagg ccgagagggc cgtccgcgcc
    26401 ttccacgaga agctctgcgc gacgcgcgtc ctcgaccccg cctgcggcac cggcaacttc
    26461 ctctacgtga cgctcgacct cttcaagcgg ctggaggccg aggtcgtgag gctcctccgc
    26521 gacctgcgcc ccgagaccgg ggcgagcggc ttcctcgacc tccacggcgt cagcgtcacg
    26581 ccccgccagc tcctcggaat cgaggtgaag cgctgggcca aggagatcgc cgagctcgtc
    26641 ctctggatcg gctggctcca gtggcacttc cggacccacg gcgacaccgc ccccgacgag
    26701 cccgtcctcc gcgactacgg gaacatcgag tgccgcgacg ccgtcctcgc ctgggaccgc
    26761 gtcgagccgg agacggacgc ggcggggaag cccgtcaccc gctgggacgg cgagacgacg
    26821 aaaccgagcc cggtgacggg ggagccgatc cccgacgaga ccgcgcgggt ggtggtggaa
    26881 aggtacgtga acccgcggaa ggcggaatgg ccggaggcgg agttcgtggt ggggaatccg
    26941 ccgttcgtgg ggaacaagcg gatgcggacg gcgctggggg acggctacgt cgaggcgctg
    27001 aggcgcgcgc acgacgacgt gccggagacg gcggacctcg tcatgtactg gtggaaccat
    27061 gcggcgtcgc tggtgcgggc agaaaggctg acgcggttcg gcctgatcac gacgaacagc
    27121 atcacgcaga cgttcaaccg acgcgtggtt cagcgacacc tcgtggcttc gtcggcatgc
    27181 catctcgcct tcgcggtacc agatcacccg tgggtcgatt cgacggatgg ggccgcggtt
    27241 cgggtcgcga tgacggttct ctcccaatcc tccggcgccg gcatcctcct ctcgctcatc
    27301 gatgaggagc agctggatga cggttcaagt cgagtcgatt tcagatcgcg gaagggcacg
    27361 attcacgcgg acctgacgac cggaccgtcg gtttccggca ctcgggcgct cgtcgcgaac
    27421 caagacctgt gccttcaggg ctgcaaactc gtgggcgacg ggttccttct gacctctgat
    27481 gaacgcgaca acttgatcgc cgttgatcat cgcgccgagg cctttcttcc gcgctacgtt
    27541 gccggccggg atctgacgca gaccgaaggc gttcgttacg tcgtggattt ctacgggttg
    27601 gacgaggcgc aagcgcgaaa tgagttcgct gccggttatc agcggctctt gcaccttgtg
    27661 aagcccgaaa gggatcagaa caagcgggat acgaggcggc gaaactggtg gttgttcggc
    27721 gagaacgcgc cgaagctgcg gcgggcgttg aaggggcttc gacggttcat cgccacttcc
    27781 gaagtcgcaa aacacagagc gttcgtgttc cttccactcc cgggaaccct ggctgatggt
    27841 tccttggccg ggatcgccac ggacgatgcc tggcacctcg gcgtcctttc ttctcgcgtc
    27901 catgtaacat gggcatttcg gagaggcggg cgaatgggcg cgggcaatga cccccgctat
    27961 cagaacgggc catgcttctt gctcttcccc ttcccgcctt ccacccccgc ccaaaccgcc
    28021 ctcatccgct ccctcggcga agccctcgac gcgcaccgca agcgccagca ggctctccat
    28081 cccggcctca cgatcaccgg gatgtacaac gtcctcgaga agctccggtc gggggaggcg
    28141 ctgacggcga aggagaaggt gatccacgag caagggctcg tctccgtcct gaagcagatc
    28201 cacgacgacc tcgacgcggc ggtcttcgac gcctacggct ggccgtcgac gctgaccgac
    28261 gaggagatcc tcgagcggct cgtcgcgctg aacgccgagc gagccgagga ggagaggcgc
    28321 ggcctcgtcc gctggctccg ccccgagttc cagaacccgg gcggcgcgaa ggcggcgacg
    28381 caggagactc ttccgggcgg agatgacgag gaggaggagc cggccgcgaa gccggccgcg
    28441 ccgctcccgt ggccgaaggc gctccccgag cggatcgccg ccgtccgcga cctgatggcc
    28501 cgcgcgccgt ccccccgctc gctcgccgcc gtgacttcgg ccttcgcgaa ggcgaagccg
    28561 aaggacgtgg agcccgtcct cgagagcctc gcctccct
//
LOCUS       DAOONL010000036        28107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1488_length_28107_cov_16.970911, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000036 DAOONL010000000
VERSION     DAOONL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1488_length_28107_cov_16.970911"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLP50_07540"
     CDS             <1..846
                     /locus_tag="PLP50_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51440.1"
                     /translation="VGAPAFAFDTRPAVCFPWANARGADGSVVPSPLLLEVLREARGS
                     TIHAATLSLATWRDDPAGFAVLTGRKLASAFNGAEIPDNASYPFFRDRLRWLAVLPVF
                     ACLLGTGVVGLLLAARRRLFRPGEAALVTIAALVPLAACLLVSTTTRYRSAAAGPLAL
                     GSGLFLGFTAEALARRRFAGAVVPASAATLLSLQTLLPSPVPCPKHRWSDALVAATLA
                     ESRFSPEAGAAEVRRYLEEGQDDPERGLGMIAMNRWLAGERTARVAAPGVAPVGRRYS
                     AARNR"
     gene            870..2504
                     /locus_tag="PLP50_07545"
     CDS             870..2504
                     /locus_tag="PLP50_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51441.1"
                     /translation="MSRRTALILVVASTVVLVAGVRLCAHRERTDSPLSSWHRWSETD
                     EWAAVAAAKRIAAGNVLDRPAFRPYFTWQRRFGSEAEWNAVLPANVAYQGPAYPYLLA
                     LAGAPAGGALEAVRLAQLLLAVVAAGSVAAAGFAVLLRSGRTPALALGVGALAGLLHG
                     TYGPLVFLDGFLYRDGPVAHLSALLLALPLIPQRPPRARLTAFVGLLAGVSALLKQTL
                     LPLGLVAGGVFALRAEGRRARTRAAAAFAAGLLCALGPLVARNVAVGASPLAFDTRPL
                     VGIPWANAKGADGSVDASPLLMDVLREAHGSTLRAGILTLETWRDDPAGFVLLLGRKL
                     GSALNGTEVADNASFFFFRDRLPWLARMPLFTWVVGAGVTGLLLAGRRRLFRGGEGLL
                     VTVSALAPLAACLMVSTTTRYRSGASAPLALGTALLALYLFEAFRERRPLQLLPWAGL
                     AAALTIVALLPSPVRAWPWRWADTIVAATLAEERLSPEAGAAEVRRYLAENRGDPDRA
                     RGLVAMHQWLAGRREHARVEPSGIAPPSRRVSAAAR"
     gene            complement(2575..4845)
                     /gene="pnp"
                     /locus_tag="PLP50_07550"
     CDS             complement(2575..4845)
                     /gene="pnp"
                     /locus_tag="PLP50_07550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPA51442.1"
                     /translation="MKESVTALVGGRALTIENGKVAKQADGAAVVRLGDTMVLVTACY
                     ARDPKDVDFVPLTVEYKEYQYAAGRIPGGFFKREGRPTEKEILTCRMIDRPIRPLFPA
                     GYAFETQVVALVLSADGENDPDVLAINGTAAALAVNPIPFTEVLGAVRVGRIGDRFVF
                     NPTKKEREESVMDLVVVGTRQAVSMVEAGAKECSEELMLEAILEGHRELQKVIDAIEE
                     LQRRQGVVKQAFTPPAPVPAEVMAEVRRNWDGPMMEALTWKGKIESYAKIKEVKKAAV
                     SVVPEDQPEKKAQVKRAISELVEVMTRETILRDRKRLDGRAFDEVRPIEVEIGVLPRT
                     HGSALFTRGETQALVTVTLGTSDDTQLIEDLEGEFEKKFLLHYNFPPFSVGEVKRFGS
                     PGRREIGHGRLAWRSIDAVLPKEFPYTIRVVSDILESNGSSSMATICGGSLALMDAGV
                     PMDAAVAGVAMGLVAEGDQWAVLTDIAGQEDHYGDMDFKVAGTRKGVTALQMDIKVSG
                     ISREVFEKALAQAKAGRMHILDIMEKAIPTARPEISAYAPRLLTIQVPEEKIRDVIGS
                     GGKTIRALQKEYSVKIDVQDDGSVTVASVDMASAETCIEAIRALTTVPEIGTEYEGTV
                     KRVEPYGAFVEILPGLDGLVHISELAPGRVRETTDVVKLGDKAKVKIIAIDPVNGKIK
                     LSRRQALTPEEAAAEVERLGLNATPPEGEGGGDDRPPFRREGGFGHRDRGPRPGFGGP
                     RGPRRDGPGGHGGPRH"
     gene            complement(4901..5158)
                     /gene="rpsO"
                     /locus_tag="PLP50_07555"
     CDS             complement(4901..5158)
                     /gene="rpsO"
                     /locus_tag="PLP50_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPA51443.1"
                     /translation="MTPKTETIKAFERHEGDTGSPEVQVALLSQRITALTEHFKTHAK
                     DHHSRRGLLQMVGQRRRLLDYLKKRDVTRYRALIERLGLRK"
     gene            complement(5432..6361)
                     /gene="truB"
                     /locus_tag="PLP50_07560"
     CDS             complement(5432..6361)
                     /gene="truB"
                     /locus_tag="PLP50_07560"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPA51444.1"
                     /translation="MSAAPADGPSGLLLVDKPEAMTSHDVVDAVRRLLGTRRVGHTGT
                     LDPAATGLLVLCVGRAGRLQSFLTCYDKSYEGTMKFGVETDTYDREGTPVGTPHPDPR
                     PDEERLRAACSRWVGEFEQAPPPFSAKKVGGKKFYELARRGEAVPHLPKKVRVTRFDA
                     VEVTADAARFHVDCSSGTYIRSLAHDVGKELGLGAHLASLRRTRIGPFSLERARTLPE
                     LEALPPAERTSPPSWMPLSEVPLPFPAIALLPGEAVKVRRGHAVPVRVPTETTGAPWV
                     RLVASGALVALGHLEPLGRGALALAKPKIVLQD"
     gene            complement(6358..7344)
                     /locus_tag="PLP50_07565"
     CDS             complement(6358..7344)
                     /locus_tag="PLP50_07565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPA51445.1"
                     /translation="MSETDFEAVSSLLASGRRFLLTGHRNPDGDSLGSALALAQALAA
                     RGKDARVVMRDRWSSSYDGMPGVGQVVVSETLPSDWPTGWDAAIVMECPEAERPGFPE
                     LLAGTTVNVDHHPGNTRWATHNLVVEPAAAVGEVVADLLDRLEWSMTPEIATNLWVSL
                     VSDTGSFRYGNTTPRALALGARLVAEGVKPDVVNEFLFEAKPLSTVKLESLVLGTLEL
                     HDEGRVALVSLPKRFLAESGADAADGEGLVNRARGIEGVKAAALVRESDEPGVFRCSL
                     RSKGSVDVRSVAAARSGGGHRNASGCRVGGTWESVKADIAGALSAAVAAEPR"
     gene            complement(7341..7715)
                     /gene="rbfA"
                     /locus_tag="PLP50_07570"
     CDS             complement(7341..7715)
                     /gene="rbfA"
                     /locus_tag="PLP50_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPA51446.1"
                     /translation="MKERRRPRQVADVVREELARLLRTEVSDPAVGFATITDVVLSPD
                     LRSARVFVSVLGGEGAFEKSVAALNRAAPMLRGMVGRNCHLRYAPELRFEEDHSLERG
                     ARIEEILRHLPHPDDEGGEGKE"
     gene            complement(7712..8059)
                     /locus_tag="PLP50_07575"
     CDS             complement(7712..8059)
                     /locus_tag="PLP50_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPA51447.1"
                     /translation="MVVALAVFDFHLPTCRGLKEKRAFLRPLKARLRGGFEISAAEVA
                     HHDLLQRASVGVAAVGPDRSGLEPLLGKVVAFVEEWAEESGVELTSLRSEFLEYGDVG
                     DAGSSFGAEQEVS"
     gene            complement(8130..11531)
                     /gene="infB"
                     /locus_tag="PLP50_07580"
     CDS             complement(8130..11531)
                     /gene="infB"
                     /locus_tag="PLP50_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPA51448.1"
                     /translation="MKTTSAKFTVTELARMMGKPAKEVLFLLQGIGVDVKSAESVLDP
                     GTAQAILTGKTQAPKTLIVRQTPVAPPPSSGATGTPKPKAERAALKRIKIVEKAPDAE
                     PTMDAEPATAVAESAAAAPVAAEPAPAAPEPAAPEAPARAESAPAVEEAAPAKSPAVA
                     EPESAPEPPVAPAPVAKVEAAVVETVPEPKPEPKEAEAPAPVVAAAPAPAPETPRAAP
                     TEAPPAAAPAAHPARHTSSRTAATPRVAPPAAAATPAPPASAPAAGTASGAIRRTAGG
                     IQTASAASLRAAAQKPATPGARPPVPAGALGRILKRAAEPAATRPTATPGQPAAAPPP
                     TAAPSAPPAAQRPSPQASAAPAARSGVSGRAVIAPPSAATIRAVARPTPGAPVARPAT
                     PVAAPGPHSRPGMAPRPGFAPRPGFAPRPGGLGRGPMAPAMPPPVPGTTDRRRKDAVK
                     GGSAVPPKKDDKKTASKKLRTKEASALATNVRDFLGTYQEDTYEDVPGAIPGEGEEVD
                     PNRPISKSAQRRAGKRTMMTETGDVVEVKKDQSGPVFLSEGVTVKELSEKTGILAKDL
                     VKAFLGRGILAAINHPVNPTLAVEVARDFGIEAAVVSFEEDLELSRQQAQTEADAESA
                     ASTADKKPRAPVVTVMGHVDHGKTSLLDSIRKAKVAEGEAGGITQHIGAYRVQAKDRP
                     IVFLDTPGHEAFTMMRARGARATDIVVLVVAADDGVMPQTVEAIDHARAAKVPVVVAI
                     NKIDKPEANVMRVKQALADKGVLVEEYGGEIVACEISAKKQIGIDALLEMILLQADLL
                     ELKAAVDGPARGVVLEARREVGRGTLATVLVQQGTLKVGDIFFAGSAVGRVRAMLDDR
                     GNRVTEAGPATPVEVMGFEDIPNSGDSLQVVDDESKARQVTGFRQQKEREEALAKSSR
                     LSLDSLFSRMAKGEVKELPIILKADVHGSEEVITQSLNKLSTAKVKVNVLHSGVGAIS
                     VNDVLLASASEAIVIGFNVRPERKAQELADKEGVDIRLYSVIYNLTDEIKKAMAGLLD
                     SVQKEVAKGRAEVRETFRIPKIGLICGCMVVDGVIPRGANARLLRDNRVIYEGKIGSL
                     RRFKDDASEVKSGFECGIGIAGYQDVKVGDVIEAFVTEEVAASLT"
     gene            complement(11528..13327)
                     /gene="nusA"
                     /locus_tag="PLP50_07585"
     CDS             complement(11528..13327)
                     /gene="nusA"
                     /locus_tag="PLP50_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPA51449.1"
                     /translation="MSSTEAPNTASRPRTARSAKAAKAAKAPPPTKAIEMLDALKAAS
                     RDKEIELDRLVSALEEAVATAARKVYKVREITARFDPATGELTAWTPYRIVEQKTKPE
                     VPAVPAIDPEDLPIGMVAPPPSAVATDADKEVRLPWIELLPEEVPGLLAGELSGQSWQ
                     IVSDPDGAASPVGPEAAAIGDEIRLFRSTEGLGRIAAQSAKQVLYQKVREAERDNIYN
                     EYAPKLGDLITGTVKRFERGDMIVDLGRTEAVIPREHQSRAERYTQGERVRGVLVDVH
                     RNPKGAQVVLSRTAPELLMKLMEMEVPEIYDGTVVIRGCVRQPGDRAKVAVSSRERDV
                     DPVGACVGMRGARVQAITRELRGERIDIIQFSEDIIAYAQNALSPAKITRVSLMAAED
                     EAAGEGEGEGEGEETMPTLECIVEEDQLSLAIGKRGQNVRLASALVGARIDIKSEQAV
                     KEQVAQALQRMLLASQRRGTALSDVAGLEPAALEALAAGGFETVGEVLDADTGEDEEA
                     MGRLDAAIADEDSREEALEALRAFESAPAEEVEEDEDDFLSSDDESPEEATGEDEDGS
                     PESVVDDGPADETEETASEEPGNAGPDTENPEK"
     gene            complement(13374..13856)
                     /gene="rimP"
                     /locus_tag="PLP50_07590"
     CDS             complement(13374..13856)
                     /gene="rimP"
                     /locus_tag="PLP50_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPA51450.1"
                     /translation="MTTLTRPELPIRAETLGRIREALAEKGLELCHVGWVPGARKGRL
                     TLTIDRPEGVTLDDCEAASHLADPILEGDETIPGSWLLEVESPGLDRPLFTLDDCRRF
                     AGRRVRVTTDVPVDGAKRMKGVLEGVDGESLVVMDEDRKRRYTVRFGDVKVVRLIPEF
                     "
     gene            14193..15266
                     /locus_tag="PLP50_07595"
     CDS             14193..15266
                     /locus_tag="PLP50_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPA51451.1"
                     /translation="MPLLALDLEEAAVGEAVPSRWSFTRDRAVFLSVLLHALFGLFVV
                     LAPTPEKPSNPDELPPDLLGLNKLWAVPPPNPPIPVHFYPAPGPKAPEPGPNPRLSDL
                     DRKAGGGDPRLPKADTPKAVAGPGIRDLAPGRRGRGRGPGDEARAAAEAVRLAAAATA
                     AERGAGGGAPNVSEDGKGTPAAKGLAGLAASPLRQIGPGQTASSAAATSRSGSPGGGG
                     DEGGGWESEGGFVDSGPLSFDTKGYDWGAYAAEMIRKIKRNWEIPKLAHYGLKGRLVV
                     RFYLLRDGRVEGLRLLVSSGVPPFDNASMQAIRSSNPFRPLPADFPNPREGVTITFLY
                     NIRPEDEGKAGTPAEPPRPLLPR"
     gene            15367..16884
                     /locus_tag="PLP50_07600"
     CDS             15367..16884
                     /locus_tag="PLP50_07600"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPA51452.1"
                     /translation="MQTRTKVLSLTALSLAAVVFGMILAGSLDFTRAASADAATPAVA
                     PAVASAPARVAPVGLPSFADIADRAMPAVVSIRSTDIIKPDQRRRGMERFFGDTPFHP
                     FFPDRRQDSGEPEEEQQLSGGTGFLIEADGFVLTNNHVIDGADKVEVTVGETDVYKAT
                     IVGRDPATDLALLKIEGSRPFPILPLGDSDRIRVGEWVMAIGDPLQFEKTVTVGVVSG
                     KGRAPGLSNVTRSFENLIQTDAAINFGNSGGPLVNVAGEVVGINTAISRLAQNIGFAV
                     PINVARRLIPQLKEGKVVRGYLGVTIGPVDADMQDAMGLPSRDGALVQSVEPGKPADK
                     AGIRHEDVIIRVDQTPVRNNRDLIDYVSGKRPGTKVTLSFLRGGKEMTAVATLETRTE
                     EGQAAEEEPAEDEHREKIGISVEELTPAARRNYAIGREIEGVVLTHVKNVSAAADAGL
                     REGDVIQEVNGRPVGTVAAFRAELKKAGDARFARFYVHRPGPRPANFVVAVRLKD"
     gene            16993..17499
                     /locus_tag="PLP50_07605"
     CDS             16993..17499
                     /locus_tag="PLP50_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51453.1"
                     /translation="MTAETKVLYDIRTEPAPIGGTIVSGLGGTLVGVVLLAVVWRRRE
                     RAARAFALLWILGWGGLSAFTAYGIASRHREARSWLESRSVEVVEGVVDQLSPATSDQ
                     KGIETFRIGDRLFRIEDGQTKTPGLNRSSVRGGPIRSRATVRLTIHDGTILRVEELAP
                     PPPGTVAR"
     gene            complement(17516..18805)
                     /gene="mqnE"
                     /locus_tag="PLP50_07610"
     CDS             complement(17516..18805)
                     /gene="mqnE"
                     /locus_tag="PLP50_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HPA51454.1"
                     /translation="MTPSGAGAPAGSFETAAAPEPETAERLLGRLADPALRRVAERSL
                     AGERLSFEDGLVLFRSPDLLGVGALANRERERRHGNRGYYNVNRYLNPTNLCWVDCGL
                     CAWARKPGVAGGYTMAIEEAVREAAAGWHDGITELHVVGGLHPSLPYDYYPGLLRMLR
                     ARFPGVHIKAWTMVELDWFARVGKKDLVETIRELKDAGMDSCPGGGAEIFAKRVRDVI
                     CTNKIDGERWLEIARLCHLEGVKTNVTMLYGSIETDEERVDHLLRIRGLQDETKGFLG
                     FVPLAFHPDGTPFAHLPATSGTLDLRVIAAARLLLDNVDHVKAYWVQIGEKFAPVALH
                     FGADDLVGTVTEETITHAAGATTEAGLSRLEMERLIRSAGREPFERDAYYERVVRDEG
                     GRVVRANRHDFVPASPSAPAPAPAHPPAPLDPALASS"
     gene            complement(18802..19050)
                     /locus_tag="PLP50_07615"
     CDS             complement(18802..19050)
                     /locus_tag="PLP50_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51455.1"
                     /translation="MSGPVWKLLRMTGSCDSCGESADELLGVGTVVDYAFRPDYRFCR
                     ACYDRYEGRHDELHPRAVAEQQLPPLISRPDSVEDVPE"
     gene            complement(19061..20263)
                     /locus_tag="PLP50_07620"
     CDS             complement(19061..20263)
                     /locus_tag="PLP50_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51456.1"
                     /translation="MESSTGTHSPSRLPRTVLALGAVSFLTDASSEMIFPLLPLLLAS
                     LPGAPAAALGVIEGVAEATAAGVKVVSGRLSDRAARRKPLVVAGYALSSAVRPLVALA
                     ALWPHVLLVRFLDRIGKGVRSAPRDALIAEVTPPDRRGAAYGLHRAMDNAGAVAGPLV
                     AAALLGLLALPLRTVLALSFVPAALALLVLVLGVRETPRSAAASAAVVRAPAAPLPPA
                     FRRAVAAVALFTLSASSDTFLLLKAREVGVAAAGLPLVWALSNAIRAALGTWGGGLSD
                     RHGRKGVLFAAWTLYAACYTLFAFVKTPLALLVVLAAYSVHAALSEGAERALVADLVP
                     PESRGRAFGWFHGAVGLAALPASAGFGLLWTRFGSRTAFLAGAGVALLAAVFLLLAVP
                     QRTRPPVR"
     gene            20250..20999
                     /gene="recO"
                     /locus_tag="PLP50_07625"
     CDS             20250..20999
                     /gene="recO"
                     /locus_tag="PLP50_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPA51457.1"
                     /translation="MLLSTTAFILRVDALSEKDVAATLLTRDVGVLRAAFRGARRRFR
                     HGALLELLTEVSVTLFRKEGAELARGDGLELVKSAFGLASKPETAMLLPYVAESLSTF
                     LPEAEAAPEAYRLARHVLDALEEGAPPALAARYFEAWLLRLAGLLPGGAECASCGRSL
                     ASGGVRFDAELPGLVGDECAAPGTVPVSAAVLRLLASVRRSRLDDLVRRGAGDPESLR
                     ALDDLLREVRRRFLGHELKSHRFLDSLSGGP"
     gene            21067..21981
                     /gene="glyQ"
                     /locus_tag="PLP50_07630"
     CDS             21067..21981
                     /gene="glyQ"
                     /locus_tag="PLP50_07630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPA51458.1"
                     /translation="MDFQTLILKLSEYWASRGCILEQPYDTEVGAGTMHPATFLRVLG
                     PEPWRCAYVQPSRRPADGRYGENPFRLVKHYQFQVVIKPSPADIQDLYVDSLRAIGIE
                     PAEHDLRFEEDNWESPTLGAWGVGWQVLLDGMEITQFTYFQQAGGIDLAPITAEITYG
                     LERLTMFLTRTRNVMDIEWAPGVPYGEVRKQDEFEVSKYGFEVADTDLLRQLFERLEA
                     ESGRCLASGLVVPAYETALKCSHAFNLLDARGAVSATERVGVIKRVRDLAVGCAKAWV
                     AARESLGHPLLPKETPMSEAAEAGDGEA"
     gene            22141..>22377
                     /locus_tag="PLP50_07635"
     CDS             22141..>22377
                     /locus_tag="PLP50_07635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPA51459.1"
                     /translation="MSEFLLEILAEEIPAGVLSQARNDLLRGLADALAAERIDGTFFA
                     HSTSRRLVLFSRDLPEAQEDHEVEAIGPSVKVAYD"
     assembly_gap    22378..22477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22478..24365
                     /gene="glyS"
                     /locus_tag="PLP50_07640"
     CDS             <22478..24365
                     /gene="glyS"
                     /locus_tag="PLP50_07640"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPA51460.1"
                     /translation="QEDHEVEAIGPSVKVAYDAEGRPTRAAEGFARGQGVAVEALRVV
                     ATPKGEYVSVRKVVEGRFTSEILGEVVPTVVAKMSFPRMMRWGDGRDVWIRPVHSVVC
                     LLDGLVVPMTLFGIESGRTTFGHRTLAPGRIVVVGVDDYLAKLRAAHVEPDYGVRRRT
                     LLEKAEALASEAGCTPVEDVALLDSLAHLVEWPGLVRGSFEAGYLELPEEILVTAMRE
                     HQKVLPTRDAAGALAPHFLAVADHVGDPMGHIVRGNEWVLNARFADARFFWDEDGRVR
                     LEERVPRLARLSFQEKLGDTLRKAERVEALSARLAADLGLEASADEAARAARLARADL
                     VTDMVREFPDLQGVVGGLYARRDGESETVCRAVYDHYRPAGVFDALPGEPVGRVVALA
                     DRLDTLAGFFALGLVPTGSRDPYALRRAALAVVRILLEGGHRLDLPVAITAARRLHEV
                     SGASEEEVVSTLHPFLLERLRFLLERRGHRHDEIEAVLTTSCPDVTDAAERVAAVSRV
                     RRQPDFGALAASFKRVQNILVQAGVASGGEIDPALMSDDAERQLAGDFFQARGILDEL
                     IASRRYDEALSVMASLGPVLDRFFVDVMVLVEDEAVRRNRLSLLAAMRDQFFRVARLS
                     EIQE"
     gene            24483..25052
                     /locus_tag="PLP50_07645"
     CDS             24483..25052
                     /locus_tag="PLP50_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HPA51461.1"
                     /translation="MRTTRRAVALSAVFAFALSAASAEARELAGASMPDTLSVAGKTL
                     KLNGMGLRKKAVFKVYVGGLYLETPSSDAAAILAADAPKAVRMHFLRSVDRGKLVETF
                     REGFAANAGAKAEAQKAGIEKLLATVTDVKDGSVTTYVYAPGAGTVVNRDGKDVATIE
                     GKEFAEVLFSLWLGPKPPSEDLRSGMLGR"
     gene            25103..27196
                     /locus_tag="PLP50_07650"
     CDS             25103..27196
                     /locus_tag="PLP50_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPA51462.1"
                     /translation="MNLPELSSLLSEKKFDVVENAVKDALLDAEENAELLAAAFRGLA
                     RASGQKGRLQGLSTLADATLRGPGTSLAAARLRWSLLKEAVRAGATPSSPEGFHKLFE
                     EAIRAAYPESASLTTLLGRFRFLESKEPADGLARVERAEKWLPFEVGRCFFMAVRGAG
                     KVVETNFALDAVRVDFEKSVGVSIPIGVAAKSLTPLPEGHFLHEKLTAREALAESVLS
                     DPSEGLRRLLDSFGRALPVAEVKEALAGLVPEERWTSWWTAARKSPHVVLHGSGKNAS
                     VEWTRTSQAADAAILAKFGKAHTPEARLDFFRKNAKRDPALAAQMGTKLVEDARALRD
                     ANPPLAYEIAVAIEKVEGVASGFAPEDLVPEAPLPMLGRIGDRLARERFLEGLLASRP
                     EDGARLVAEWFFREEDARTLDLIDRRLAEVDPATREATLEKLLKSPRSGPKAFLWFGL
                     RSAQDDAFRARLTPTVLGRLLDAASWEDLGGGRAKLRELFDRTGVAAAWLVKQATLEE
                     AQQFLQALDRHNELEPHRRAALFAAAEMRFPDLRKGGDDSFFVTDEALEGKKKELERL
                     VEVDIPENTKGLAIARAEGDLSENFEYQARKQRQQDLGVRVARLQEELRKARVLDPAT
                     VDTTEVRPGTRVTLRLLGGTRAVTLLGPWDSRPEADVYSYLADASKGLLGRKVGDRVS
                     FLGDEGLIEAIEVWK"
     gene            27284..>28107
                     /locus_tag="PLP50_07655"
     CDS             27284..>28107
                     /locus_tag="PLP50_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPA51463.1"
                     /translation="MRSRILARARRIWRAIPRWALWGAAGLVLALAVVSAFVVAYYAR
                     VVSRTMDGRRWNLPTRLYSDAWVLRPGDALGPDDVERRLARLRYAPVAELPVSGGRYL
                     RTRSRIVVGVNPRETAYGRSPGLAARIDFSGRRISALRREPDGAPIPYVVFEPEVVGS
                     VFDRKMEDRTLVRLERVPKVVVDAIVTTEDRDFFTHAGLDPTRLAGALLQGIARGRKV
                     RGTSTLTQQLVKNLFLTPERTLRRKAVEALLAVILDAKYGKEEILEAYLNEIYLGQR"
BASE COUNT         3687 a         9897 c        10351 g         4072 t
ORIGIN      
        1 gtcggggcgc ccgcgttcgc cttcgacacg cggccggccg tctgcttccc atgggccaac
       61 gcccgcggcg ccgacggctc ggtggtcccc tcgcccctcc tcctcgaggt cctgcgtgag
      121 gcgcggggat cgacgatcca cgcggcgacg ctctccctgg cgacctggcg cgacgacccg
      181 gccggcttcg ccgtcctgac cgggaggaag ctcgccagcg ctttcaacgg cgccgagatc
      241 cccgacaacg cgagctaccc gttcttccgc gaccggctcc ggtggctggc ggtgctcccc
      301 gtcttcgcgt gcctgctcgg gaccggcgtc gtcggcctgc tccttgcggc ccgccgccgc
      361 ctcttccgtc caggcgaagc cgctctcgtc accatcgcgg ccctcgtccc cctcgcggcg
      421 tgcctcctcg tctcgacgac gacgcgctac aggagcgccg cggccggacc gctcgctctc
      481 ggctcggggc tcttcctcgg cttcacggcc gaggcgctcg cgagacgccg gttcgcaggg
      541 gcggtggtgc ccgcctcggc ggccacgctc ctctccctgc agactcttct cccgtcgccg
      601 gtcccgtgcc cgaagcaccg atggtccgac gccctcgtcg cggcgacgct cgccgagtcg
      661 cggttctcgc ccgaggccgg cgccgcggag gtccgacgct acctggagga gggccaggac
      721 gaccccgagc gcggcctcgg gatgatcgcg atgaaccgct ggctcgccgg ggagcgcacg
      781 gcgcgcgtcg ctgctcccgg cgtcgcgccg gtcgggcgcc ggtactccgc ggcccgaaac
      841 cggtgagccg gcgatcggac ggggcggacg tgtcgcgtcg aacggccctc atcctcgtcg
      901 tcgccagcac cgtcgttctc gtcgccggag tccgcctctg cgcccatcgc gagcgcacgg
      961 actcacccct ctccagctgg catcgctggt ccgagaccga cgagtgggcc gccgtcgccg
     1021 ctgcgaagcg aatcgccgcc ggaaacgtcc tcgaccggcc ggcctttcgg ccctacttca
     1081 cctggcagcg gcgattcgga agcgaagcgg aatggaacgc cgtcctgccg gcaaacgtcg
     1141 cctaccaggg gccggcctat ccctatctgc tcgccctggc gggtgccccg gcggggggcg
     1201 ccctcgaggc ggtccgtctc gcacagctcc tcctcgccgt cgtcgcggcg ggcagcgtcg
     1261 cggcggcggg cttcgcggtc ctcctgcgct cagggcggac tccggccctg gccctcggcg
     1321 tcggcgccct cgcaggcctc ctccatggca cctacgggcc gctcgtcttc ctcgacgggt
     1381 tcctctatcg cgatgggccg gtcgcgcacc tctcggccct cctgctcgcc cttcccctca
     1441 ttccgcaacg tccgccccgg gcgcgcctga cggcgttcgt cggccttctg gccggcgttt
     1501 ccgccctcct gaagcaaacg ctcctgccgc tcggcctcgt cgcgggaggg gtcttcgccc
     1561 tccgtgcgga ggggcggagg gcgaggacgc gggcggcggc ggccttcgcg gctggtctcc
     1621 tctgcgccct cgggccgctc gtcgcgcgga atgtcgccgt cggcgcgtcc ccgctggcct
     1681 tcgacacgcg acccctcgtc ggcatcccct gggcgaacgc gaagggggcg gacgggtcgg
     1741 tggacgcctc gcccctcctg atggatgtgc tgcgagaggc tcatggctcg acgctgcgcg
     1801 cgggcatcct gaccctcgag acatggcgcg acgacccggc ggggttcgtc ctgctcctcg
     1861 gccggaagct cgggagcgcc ctgaacggca cggaggtggc cgacaacgcc agcttcttct
     1921 tctttcgcga ccggctgccg tggctggcgc ggatgccgct cttcacctgg gtcgtcggcg
     1981 cgggcgtcac cggcctgctc ctggccgggc gccgacgcct gttccggggt ggggaggggc
     2041 tgctcgtgac cgtgtcggcg ctcgccccgc tcgccgcgtg cctgatggtg tcgacgacga
     2101 ctcgctacag gagcggcgcc tccgcgcccc tggcgctcgg cacggcgctt ctcgcgctct
     2161 acctcttcga ggcgttccgc gaacgccggc cgctccagct cctcccgtgg gccggcctcg
     2221 cggcggccct gacgatcgtg gcgctcctcc cctcgcctgt ccgcgcctgg ccgtggcgct
     2281 gggccgacac gatcgtcgcc gcgacgctgg ccgaggagcg gctctcgccg gaagcgggcg
     2341 ccgcggaggt gaggcgctac ctcgcggaga accgcgggga tccggatcgg gcgcgtgggc
     2401 tcgtcgccat gcatcagtgg ctcgccggac ggcgggagca cgcgcgggtc gagccctccg
     2461 ggatcgcccc gccgtctcgg cgcgtctcgg cagcggcccg ctgaggaagc cggatgaaaa
     2521 gagagaaggc cgggctttcg cccggccttc gtggacgctc agggagcgcg aagctcagtg
     2581 acgcgggccg ccgtggccgc ccggtccgtc gcggcgcggg ccgcgcgggc cgccgaagcc
     2641 ggggcgcggg ccgcggtcgc gatggccgaa gcccccttcg cggcggaagg gcggacggtc
     2701 gtcgccgccc ccttcgccct cgggcggggt ggcgttcagc ccgaggcgct cgacctcggc
     2761 ggccgcctcc tcgggggtga gcgcctggcg gcgggagagc ttgatcttcc cgttgaccgg
     2821 gtcgatcgcg atgatcttga ccttggcctt gtcgccgagc ttcacgacgt ccgtcgtctc
     2881 gcggacgcgc ccgggggcga gctcggagat gtggacgagg ccgtcgaggc cggggaggat
     2941 ctcgacgaac gcgccgtagg gctcgacgcg cttgacggtc ccttcgtact cggtgccgat
     3001 ctcggggacg gtcgtgaggg ctcggatcgc ctcgatgcat gtctcggccg aggccatgtc
     3061 gacggacgcc acggtgacgg agccgtcgtc ctggacgtcg atcttgaccg agtactcctt
     3121 ctggagcgcc cggatcgtct tgccgccgga gccgatgacg tcgcggatct tctcctcggg
     3181 gacctggatc gtcaggaggc ggggcgcgta ggccgagatc tcggggcgcg ccgtcgggat
     3241 cgccttctcc atgatgtcga ggatgtgcat ccggccggcc ttcgcctgcg cgagcgcctt
     3301 ctcgaagacc tcgcggctga tgccggagac cttgatgtcc atctggaggg cggtgacgcc
     3361 cttgcgcgtg ccggcgacct tgaagtccat gtcgccgtag tggtcctcct ggccggcgat
     3421 gtcggtcagg acggcccact ggtcgccctc cgcgacgagt cccatcgcga cgcccgcgac
     3481 ggcggcgtcc atcgggacgc cggcgtccat gagggcgagc gaaccgccgc agatcgtggc
     3541 catcgaggag gagccgttcg actcgaggat gtcggagacg acgcggatcg tgtaggggaa
     3601 ctccttcggc aggacggcgt cgatggagcg ccaggcgagc cggccgtggc cgatctcgcg
     3661 ccggcccggg gagccgaagc gcttcacctc tccgacggag aacggcggga agttgtagtg
     3721 gaggaggaac ttcttctcga actcgccctc gaggtcctcg atgagctggg tgtcgtccga
     3781 ggtcccgagc gtgacggtga cgagcgcctg cgtctcgccg cgcgtgaaga gcgcggagcc
     3841 gtgcgtgcgc gggaggacgc cgatctcgac ctcgatgggg cggacctcgt cgaacgcgcg
     3901 gccgtcgagc cgcttgcggt cgcggaggat cgtctcgcgc gtcatcacct cgacgagctc
     3961 ggagatcgcc cgcttgacct gggccttctt ctccggctgg tcctcgggga cgaccgagac
     4021 ggcggccttc ttgacctcct tgatcttcgc gtacgactcg atcttcccct tccaggtgag
     4081 ggcctccatc atcggcccgt cccagttgcg gcggacctcg gccatcacct cggcggggac
     4141 cggcgcggga ggcgtgaagg cctgcttgac gactccctgg cggcgctgga gctcctcgat
     4201 cgcgtcgatg accttctgga gctcgcggtg cccctcgagg atcgcctcga gcatcagctc
     4261 ctcggagcac tccttcgcgc ccgcctcgac catcgagacg gcctggcggg tgccgacgac
     4321 gacgaggtcc atcacggact cctcgcgctc cttcttcgtc gggttgaaga cgaagcggtc
     4381 gccgatgcgg ccgacgcgga cggcgccgag gacttccgta aacgggatcg ggttcacggc
     4441 gagggcggcg gccgtgccgt tgatcgcgag gacgtcgggg tcgttctcgc cgtcggccga
     4501 gaggacgagg gcgacgacct gcgtctcgaa ggcgtacccg gccgggaaga gcggccggat
     4561 cggccggtcg atcatccggc aggtcaggat ctccttctcg gtagggcgcc cctcgcgctt
     4621 gaagaatcct ccggggatgc gcccggcggc gtactggtac tccttgtact cgaccgtgag
     4681 cgggacgaag tcgacgtctt tcgggtcgcg ggcgtagcag gcggtgacga ggaccatggt
     4741 gtcgccgagc cggacgacgg cggcgccgtc ggcctgcttg gccacctttc cgttctcgat
     4801 cgtgagggca cggcctccca ccagggcggt gacggattcc ttcatcgttt ccttctttcg
     4861 gccggagagg ccgcggcgcg gccccgcgcc ggcacggctc ctacttgcgg agcccgaggc
     4921 gctcgatcag ggccctgtag cgcgtcacgt cgcgcttctt gaggtaatcg agcaggcggc
     4981 gccgctggcc gaccatctgg agcagtccgc ggcgggagtg gtggtccttc gcgtgcgtct
     5041 tgaagtgctc ggtcagggcc gtgatccgct gcgagaggag cgccacctgg acctcggggg
     5101 agccggtgtc gccctcgtgc ctctcgaagg ccttgatcgt ttccgtcttg ggagtgatgg
     5161 tctgggccac ggttctctat ccttctcttc tcaatctcga acggcagagg ataaggtccg
     5221 ccgtcgatcg ggtcaatttc ggggccaagt ctctgtccgg tgagaagatg ccgtctggcc
     5281 cgttgtcggt ccgggtgtct gggaagggag ccggagccgg gccgacaggc cccggatccc
     5341 ccgggaaaac gattctatct gtccgcccgc gcggaggggg tccgggggaa ccggcgtcgg
     5401 gccgagaggc cccggatctc ccgggaacac ttcagtcttg tagcacgatc ttcggtttcg
     5461 cgagagcgag cgctccgcgg ccgaggggct cgaggtgccc gagggcgacg agggcgcccg
     5521 aggcgacgag ccggacccag ggtgcccccg tcgtctccgt cgggacgcga acggggacgg
     5581 cgtgccctct ccggaccttg accgcctcgc cgggaaggag cgcgatggcc ggaaagggga
     5641 gcgggacctc cgagagcggc atccagctcg gcggggaggt cctctcggcg ggagggagcg
     5701 cttcgagctc gggcagcgtc cgcgcccgct cgagggagaa ggggccgatg cgggtccggc
     5761 gaagggacgc gaggtgcgcg ccgaggccga gctccttgcc gacgtcgtga gcgagggacc
     5821 ggatgtaggt cccggaggag cagtcgacgt ggaagcgcgc cgcgtcggcc gtgacctcga
     5881 cggcgtcgaa gcgcgtgacc cggaccttct tcggcaggtg cgggacggct tcgccgcggc
     5941 gggcgagctc gtagaacttc ttcccgccga ccttcttcgc agaaaagggc gggggggcct
     6001 gctcgaactc gccgacccag cgggagcagg cggcccgcag ccgctcctcg tcgggccgag
     6061 ggtcggggtg cggcgtcccg acgggagtcc cctcccggtc gtaggtgtcg gtctccacgc
     6121 cgaacttcat cgtcccctcg tagctcttgt cgtagcaggt gaggaacgac tggaggcgtc
     6181 cggcccggcc gacgcacaag acgagaagac ccgtcgcggc ggggtcgagg gtcccggtgt
     6241 gtccgacgcg ccgcgtgccg agcaggcgcc gcacggcgtc cacgacgtcg tgcgaggtca
     6301 tcgcctcggg cttgtcgacg aggaggagcc cggacgggcc gtcggccgga gcggcgctca
     6361 tcgcggctcc gcggcgaccg cggcggagag cgcgccggcg atgtcggcct tcacggactc
     6421 ccaggtcccg cccacgcggc agccggaggc gttccggtgt ccgccgccgc ttcgcgcggc
     6481 agcgacggac cggacgtcca cggagccctt ggagcggagc gagcagcgga agacacccgg
     6541 ctcgtcgctc tccctcacga gggcggcggc cttcacgcct tcgatcccgc gagcccggtt
     6601 cacgagcccc tcgccgtcgg ccgcgtcggc ccccgactcg gcgaggaacc gcttcggcag
     6661 ggagacgagc gcgacgcgcc cctcgtcgtg gagctcgagc gtgccgagga cgagcgactc
     6721 gagcttcacg gtcgagagcg gcttcgcctc gaagaggaac tcgttgacga cgtcgggctt
     6781 cacgccctcc gctacgagac gcgcgccgag cgcgagggcg cggggggtcg tgttgccgta
     6841 gcggaaggaa cccgtgtcgg agacgagcga gacccagagg ttcgtggcga tctcgggcgt
     6901 catcgaccac tcgaggcggt cgaggaggtc ggcgacgacc tcgcccaccg cggcggccgg
     6961 ctcgacgacg aggttgtggg tcgcccatct cgtgttgccg gggtggtggt cgacgttcac
     7021 ggtcgtcccg gcgagcagct ccggaaagcc cgggcgctcg gcctcggggc actccatcac
     7081 gatcgccgcg tcccagccgg tcggccagtc ggacggcagg gtctcggaga cgacgacctg
     7141 gccgacaccc ggcatcccgt cgtacgagct cgaccagcgg tcccgcatga cgacgcgcgc
     7201 gtccttgccg cgcgcggcga gcgcctgcgc gagcgccagg gccgagccga gcgagtcgcc
     7261 gtccgggttc ctgtggcccg tcaggaggaa gcgccggccg gaagcgagga gagacgagac
     7321 ggcctcgaag tccgtctcgc tcattccttc ccctctccgc cctcgtcgtc gggatgcggc
     7381 aggtgtcgga ggatctcctc gatgcgcgcg ccgcgctcga ggctgtggtc ctcctcgaag
     7441 cggagctcgg gggcgtaacg caggtggcag ttgcgcccga ccatgccgcg gagcatcggg
     7501 gcggcccggt tcagcgcggc gacgctcttc tcgaacgccc cttcgccccc gaggaccgag
     7561 acgaagaccc gggcggagcg gaggtcgggg gagaggacga cgtcggtgat cgtggcgaag
     7621 cccacggcgg gatcggagac ctccgtccgg aggaggcggg cgagctcctc gcggacgacg
     7681 tcggcgacct ggcggggacg gcggcgttcc ttcacgagac ctcctgctcg gctccgaacg
     7741 aggagccggc atcgccgacg tccccgtatt cgagaaactc gctgcgcagc gaggtgagct
     7801 cgactccgga ctcctcggcc cactcctcga cgaaggcgac gaccttcccg aggaggggct
     7861 cgagaccgga gcggtccgga ccgaccgcgg cgacgccgac ggaggcgcgc tggagcaggt
     7921 cgtggtgggc gacctcggcg gccgagatct cgaagccgcc gcgaaggcgc gccttgagcg
     7981 gccggaggaa ggccctcttc tccttgagcc cgcggcaggt cgggaggtgg aaatcgaaaa
     8041 cggcgagggc gacgaccatc ggttctccgg ggcgacgagg ttccgggacg tccttcccgg
     8101 ggcgcgtgcc ccgggaagga cgtccggccc tacgtcagcg aggcggcgac ctcctcggtc
     8161 acgaaggcct cgatgacgtc gccgaccttg acgtcctgat agccggcgat gccgatgccg
     8221 cactcgaagc cgctcttgac ctcggaggcg tcgtccttga aacggcgcag ggagccgatc
     8281 ttcccttcgt agatcacgcg gttgtcgcgg aggagacggg cgttcgcgcc gcgcgggatg
     8341 accccgtcga cgaccatgca gccgcagatg aggccgatct tcgggatgcg gaaggtctcg
     8401 cgcacctcgg cccggccctt cgcgacctcc ttctggacgg agtcgaggag gccggccatc
     8461 gccttcttga tctcgtcggt caggttgtag atgaccgagt agaggcggat gtcgacgcct
     8521 tccttgtcgg cgagctcctg ggccttgcgc tccggcctca cgttgaagcc gatgacgatg
     8581 gcctcggaag ccgaggcgag caggacgtcg ttcacggaga tcgcgccgac gccggagtgg
     8641 aggacgttga ccttcacctt ggccgtcgag agcttgttga gcgactgtgt gatgacctcc
     8701 tcggagccgt ggacgtcggc cttgaggatg atcgggagct ccttgacctc gccctttgcc
     8761 atccggctga agagcgagtc gagcgagagg cgcgaggact tggccagggc ctcctcccgc
     8821 tccttctgct ggcggaagcc ggtgacctgg cgggccttcg actcgtcgtc gacgacctgc
     8881 agggaatcgc ccgagttcgg gatgtcctcg aagcccatga cctcgacggg ggtggcggga
     8941 ccggcctcgg tgacgcggtt gccccggtcg tcgagcatcg cgcggacgcg gccgacggcc
     9001 gagccggcga agaagatgtc gccgaccttg agggtgccct gctggacgag gacggtggcg
     9061 agcgtgccgc ggcccacctc gcgacgggct tcgaggacga cgccgcgggc gggcccgtcg
     9121 acggcggcct tgagctcgag gaggtcggcc tgcaggagga tcatctcgag gagggcgtcg
     9181 atcccgattt gcttcttcgc ggagatctcg caggcgacga tctcgccgcc gtactcctcg
     9241 acgaggacgc ccttgtcggc gagggcctgc ttgaccctca tgacgttcgc ctcgggcttg
     9301 tcgatcttgt tgatggcgac gacgacgggg accttcgccg cgcgggcgtg gtcgatcgcc
     9361 tcgacggtct gcggcatgac gccgtcgtcg gccgcgacga cgaggacgac gatgtcggtc
     9421 gcccgcgcgc cgcgggcgcg catcatcgtg aaggcctcgt ggcccggcgt gtcgaggaag
     9481 acgatcggcc ggtccttggc ctggacgcgg taggcgccga tgtgctgcgt gatgccgccg
     9541 gcttcgccct cggccacctt cgccttgcgg atggagtcga ggagggacgt cttgccgtgg
     9601 tcgacgtggc ccatgacggt cacgaccggg gcgcggggct tcttgtcggc cgtggaggcg
     9661 gcgctctccg cgtcggcctc ggtctgcgcc tgctggcggg agagctcgag gtcttcctcg
     9721 aacgagacga ccgccgcctc gatgccgaag tcgcgggcga cctcgacggc gagcgtcggg
     9781 ttcacgggat ggttgatcgc ggcgaggatg ccgcggccga ggaacgcctt gacgaggtcc
     9841 ttcgcgagga tgccggtctt ctcggagagc tccttgacgg tgacgccctc ggagaggaag
     9901 acggggccgc tctggtcctt cttgacctcc acgacgtcgc cggtctcggt catcatcgtc
     9961 cgcttgccgg cgcggcgctg ggccgacttg gagatcgggc ggttcggatc gacctcctcg
    10021 ccctcgcccg ggatcgcgcc cgggacgtcc tcgtacgtgt cttcctgata ggtgccgagg
    10081 aagtcgcgga cgttcgtggc gagagccgaa gcttccttcg tccggagctt cttgctggcg
    10141 gtcttcttgt cgtccttctt cggcgggacg gcgctgccgc ccttgaccgc gtccttgcgg
    10201 cgacggtccg tggtgccggg gacgggcggg ggcatcgcgg gcgccatcgg cccgcgtccg
    10261 agcccgcccg gacgcggcgc gaagccgggg cgcggagcga agcccggtcg aggggccatc
    10321 ccgggcctcg agtgggggcc gggagccgcg acgggcgtcg cgggacgcgc gacgggcgcg
    10381 ccgggggtgg ggcgcgcgac ggcgcggatc gtcgcggcgc tcggcggcgc gatgacggcg
    10441 cggccggaaa cgccggaccg ggcggccgga gccgcggagg cctgaggaga cggtcgctgc
    10501 gcggccggcg gggcggaggg cgcagcagtc ggcggcggag ccgccgcggg ctggcccggg
    10561 gtcgccgtcg gcctcgtggc ggccggctcg gcggcgcgct tgaggattcg gccgagggcg
    10621 ccggccggaa ccggaggccg ggcgccgggc gtggcgggct tctgagcggc ggcgcgcagg
    10681 ctcgccgcgg aagccgtctg gatgccaccc gccgttcgcc ggatcgcgcc gcttgcggtc
    10741 ccggcagccg gggcggacgc cggaggcgca ggggtcgccg cggccgcggg aggtgcgacg
    10801 cgcggggtcg ccgcggtccg gctggaggtg tggcgcgccg ggtgcgcggc cggagccgcg
    10861 gcgggcgggg cctcggtcgg ggcggcccga ggcgtctccg gggcaggggc cggagcagcg
    10921 gccaccaccg gagcgggggc ttcggcctcc ttgggctccg gcttcggctc cgggaccgtc
    10981 tcgacgacgg cggcctcgac cttcgccacg ggggccggcg cgaccggagg ctcgggggca
    11041 ctctccggct ctgcgacggc cggggatttc gcgggggccg cctcctcgac ggcgggcgcg
    11101 ctttcggccc tcgcgggggc ctccggcgcg gccggctcgg gcgcggcggg tgccggctcc
    11161 gcggccaccg gtgcggcggc ggcggactcg gcgaccgccg tcgcgggctc tgcgtccatc
    11221 gtcggctcgg catccggcgc cttctcgacg atcttgatcc gcttcagtgc ggcgcgttcg
    11281 gccttcggct tcggcgtccc cgtggctccc gaggagggag ggggagcgac cggcgtctgc
    11341 cggacgatga gggtcttggg ggcctgcgtc ttgcccgtca ggatcgcctg ggcggtcccg
    11401 gggtcgagca ccgactcggc ggacttcacg tcgacgccga tgccctgaag gaggaagagg
    11461 acctccttcg cgggctttcc catcatccgg gcgagctcgg tcacggtgaa cttcgcggac
    11521 gtcgtcttca cttctcgggg ttctccgtat ccggcccggc gtttccgggc tcctcgctcg
    11581 ccgtctcttc cgtctcgtcc gcggggccgt cgtcgacgac ggactcgggg gagccgtcct
    11641 cgtcttcgcc tgtcgcttcc tcggggctct cgtcgtcgga ggagagaaag tcgtcctcgt
    11701 cctcctcgac ctcttcggcc ggagccgact cgaacgcgcg aagggcctcg agggcctcct
    11761 cacgcgaatc ctcgtccgcg atcgcggcat cgaggcggcc catggcctcc tcgtcctcgc
    11821 cggtgtcggc gtcgagcacc tcgcccaccg tctcgaaccc gccggcggcg agagcctcga
    11881 gcgccgccgg ctcgagcccg gcgacgtcgg agagcgccgt gccgcgccgc tgcgacgcga
    11941 ggagcatccg ctggagggcc tgggcgacct gctccttcac ggcctgctcg ctcttgatgt
    12001 cgatccgggc cccgacgagc gcggaggcga gccggacgtt ctgcccgcgc ttgccgatgg
    12061 cgagagagag ctggtcttcc tcgacgatgc actcgagcgt cggcatcgtc tcctcgccct
    12121 cgccctcgcc ctcgccctcg cccgcggcct cgtcctcggc ggccatcagg gagacgcgcg
    12181 tgatcttggc gggagagagg gcgttctggg cgtacgcgat gatgtcctcg gagaactgga
    12241 tgatgtcgat ccgctctccg cgcagctcgc gggtgatcgc ctgcacgcgc gcgccgcgca
    12301 tgccgacgca ggcgccgacc gggtcgacgt ccctctcgcg cgaggagacc gccaccttgg
    12361 ccctgtctcc cggctggcga acgcagcccc ggatgacgac ggtcccgtcg tagatctcgg
    12421 ggacctccat ctccatcagc ttcatcaaga gctcgggagc ggtccgcgaa aggacgacct
    12481 gggcgccctt ggggttgcgg tggacgtcga cgaggacgcc ccggacccgc tcaccctgcg
    12541 tgtagcgctc ggcgcgggac tggtgctccc tcgggatcac ggcttcggtc cgcccgaggt
    12601 cgacgatcat gtcgccccgc tcgaagcgct tcaccgtccc ggtgatcagg tcgccgagct
    12661 tcggggcgta ttcgttgtag atgttgtcgc gctcggcctc gcggaccttc tggtagagga
    12721 cctgcttggc gctctgggcc gcgatgcgtc cgagcccctc ggtcgaccgg aagagccgga
    12781 tctcgtcgcc gatcgcggcg gcctcgggac cgacgggcga ggcggcgccg tcggggtcgc
    12841 tcacgatctg ccagctctgg ccggagagct cgccggccag gaggccgggg acctcctcgg
    12901 ggaggagctc gatccagggg agccggacct ctttgtcggc gtccgtcgcg accgccgacg
    12961 ggggaggcgc gaccatgccg atcgggaggt cctcggggtc gatcgcgggg acggccggaa
    13021 cctccggctt cgtcttctgc tcgacgatcc ggtagggggt ccaggccgtg agctcgcccg
    13081 tcgccgggtc gaatcgagcg gtgatctccc ggaccttgta gaccttgcgc gccgccgtgg
    13141 cgaccgcctc ctcgagcgcg gagacgagcc ggtcgagctc gatctccttg tcgcgcgagg
    13201 cggccttgag ggcgtcgagc atctcgatcg ccttggtcgg gggcggggcc ttggccgcct
    13261 tggcggcctt cgcggagcgg gccgttctgg gccgggaggc ggtgttcggg gcctcggtcg
    13321 agctcatcgt gtcgggtttc tccatgcgcg gcggtgtccg gtcggtctcg ggttcagaac
    13381 tcggggatca ggcggacgac cttgacgtcg ccgaaccgaa cagtgtaacg gcgctttcgg
    13441 tcttcgtcca tgacgacgag agactcgcca tcgacgcctt cgagaacgcc cttcatcctc
    13501 ttcgcgccgt cgacggggac gtcagtcgtc acgcgaacgc gtcgtccggc gaaacgccgg
    13561 cagtcgtcga gcgtgaagag cggtcgatcg aggccgggag actcgacctc gaggagccag
    13621 gagcccggga tcgtctcgtc gccctcgagg atcgggtccg cgaggtgcga ggcggcttcg
    13681 cagtcgtcga gggtgacgcc ctccgggcgg tcgatcgtca gcgtgaggcg ccccttccgg
    13741 gcaccgggga cccacccgac gtgacaaagc tcgaggccct tctcggcgag ggcctcacgt
    13801 atgcgcccga gggtctcggc gcgaatcgga agttcgggtc gtgtcagcgt cgtcaccaat
    13861 gaaaagggcg ggcgtcgaag cccaccctca agcccttgaa agtcagctcc cagaaggtga
    13921 ttcataccac cgcgccgcct tccgggcaag ccggcgccgg gtaagatggg gcgccggaga
    13981 gccgccgggc gtggcacgga gttcccatac gccgggccga gcgcgctttc gtgtcgcccg
    14041 aacgagaatt gaagaccatc tccccaggcc tgctcggcct cgatccgtcc cgacccgggg
    14101 accagacgcc cgttccctgg ccgccgccgg gggggctcgc tccggcgcac gatcccttcg
    14161 ctcgcccggc gcccctggag gaggaagtcc ccgtgccgct gctggccctc gacctcgagg
    14221 aggccgcggt gggggaggcg gtcccatctc gctggagctt cacccgcgac agggctgtct
    14281 ttctttccgt gcttctgcac gccctgttcg gccttttcgt cgtcctcgct ccgacccctg
    14341 agaagccgtc gaaccccgat gagctgccgc cggacctcct cgggctgaac aagctctggg
    14401 cggtgccgcc cccgaatccg ccgattcccg tccatttcta tccggcgccc gggccgaagg
    14461 ccccggagcc cgggccgaat ccgcgtctct ccgacctcga tcgaaaggcg ggcgggggcg
    14521 acccgcgcct cccgaaggcg gacacgccga aagcggtcgc cggtcccggg atccgcgacc
    14581 tcgcgccggg tcgccgcggg agaggccgcg ggccgggaga cgaggcccgc gcggccgccg
    14641 aggccgttcg cctcgcggcc gcggcgaccg cggcggagcg gggagcggga ggcggggcgc
    14701 cgaacgtctc cgaggatggg aaggggacgc ccgccgcgaa ggggctcgcg ggcctcgcgg
    14761 cgagccctct ccgccagatc gggcccgggc agaccgcgag ctccgccgcc gcgacctcgc
    14821 gttccggatc gccgggggga ggcggtgacg aagggggcgg atgggagtcg gaagggggct
    14881 tcgtcgactc cggacccctc tccttcgaca cgaagggcta cgactggggg gcgtacgccg
    14941 cggagatgat ccggaagatc aagcggaact gggagatccc gaagctcgcg cactacgggt
    15001 tgaaggggcg gctcgtcgtc cgcttctacc tgctgcggga cgggcgggtc gaggggctgc
    15061 ggctcctcgt ctcgtccggc gtcccgccgt tcgacaacgc ctccatgcag gcgatccgga
    15121 gctccaatcc gttccggccg cttccggccg acttcccgaa cccccgggag ggggtcacga
    15181 tcacgttcct ctacaacatt cgcccggaag acgaggggaa ggccgggacg ccggcggaac
    15241 cgcctcgccc gcttcttccc agatagcgtc cggcgggtga caatgtcagg ttctaccccg
    15301 gcctgaacga cgcagctgtg ccgggcatgg gccttgcttc gcagggcttc gatggagaaa
    15361 gagaccatgc agaccaggac caaggttctc tcgctcacgg cgctcagcct cgcggcggtc
    15421 gtcttcggga tgatcctcgc ggggagcctc gacttcaccc gcgccgcctc ggccgacgcc
    15481 gcgactcccg cggtcgcgcc cgccgtcgcg agcgccccgg cgcgcgtcgc gccggtcggc
    15541 ctcccctcct tcgcggacat cgccgatcgc gcgatgccgg ccgtcgtctc gatccggtcc
    15601 accgacatca tcaagccgga ccagaggcgc cggggaatgg agcggttctt cggcgacacg
    15661 ccgttccacc ccttcttccc ggaccgccgc caggattccg gcgagcccga ggaggagcag
    15721 cagctgtcag gagggaccgg cttcctcatc gaggccgatg gtttcgtcct gacgaacaac
    15781 cacgtcatcg acggcgccga caaggtcgag gtgaccgtcg gcgagacgga cgtctacaag
    15841 gcgacgatcg tcggccgcga cccggcgacg gacctcgcgc tgctgaagat cgagggcagc
    15901 cgccccttcc cgatcctccc tctcggcgac tccgaccgca tccgcgtcgg cgagtgggtc
    15961 atggcgatcg gcgacccgct ccagttcgag aagacggtca cggtcggggt cgtctccgga
    16021 aagggccgcg cgccgggcct ctcgaacgtg acccgctcgt tcgagaacct gatccagacg
    16081 gacgccgcca tcaatttcgg gaactcgggc gggccgctcg tgaacgtggc aggggaggtc
    16141 gtcggcatca acaccgcgat ctcccgcctc gcgcagaaca tcgggttcgc ggtcccgatc
    16201 aacgtcgcgc gccggctcat cccgcagctc aaggagggga aggtcgtccg cggctacctg
    16261 ggcgtgacga tcggaccggt cgacgccgac atgcaggacg cgatgggcct ccccagccgg
    16321 gacggtgcgc tggtccagtc ggtcgagccg gggaagccgg ccgacaaggc cgggatccgc
    16381 cacgaggacg tcatcatccg cgtggaccag acgccggtgc ggaacaaccg cgacctgatc
    16441 gactacgtct ccgggaagcg tcccgggacg aaggtcaccc tctcgttcct gcgcggcggc
    16501 aaggagatga ccgccgtcgc cacgctcgag acgcgaaccg aggaaggcca ggcggccgag
    16561 gaggagccgg ccgaggacga gcaccgcgag aagatcggga tctcggtgga ggagctcacg
    16621 ccggcggccc ggcggaacta cgcgatcggc agggagatcg aaggggtcgt cctgacccac
    16681 gtcaagaacg tgtccgcggc ggcggacgcc gggctgcgcg agggagacgt catccaggag
    16741 gtgaacggcc ggcccgtcgg cacggtcgcc gccttccggg ccgagctgaa gaaggccgga
    16801 gacgcccggt tcgcgcgctt ctacgtccac cgccccgggc cgcgcccggc gaacttcgtc
    16861 gtcgcggtcc gcctcaagga ctgaacccgg ggcccgtccg ggagccggcc cgaaggcccc
    16921 gcgccgactg gcgcggggcc ttcgcgtttc gtccggagcg ccggccggag tttcgaggca
    16981 tcatccgggg ccgtgaccgc cgagacgaag gtgctctacg acatccggac ggaaccggcg
    17041 ccgatcggcg ggacgatcgt gagcgggctg ggcgggacgc tcgtcggcgt cgtcctcctc
    17101 gcggtggtgt ggcgtcgccg ggagcgcgcc gctcgcgcgt tcgcgctcct ctggatcctc
    17161 ggctggggcg gactctcggc cttcaccgcg tacgggattg cctcccgcca tcgggaggcc
    17221 cgcagctggc tcgagagccg ctccgtcgag gtcgtcgaag gagtcgtcga ccagctctcg
    17281 ccggcgacct ccgaccagaa ggggatcgag acctttcgga tcggcgacag gctcttccgg
    17341 atcgaggacg gccagacgaa gacaccgggg ctgaaccgga gctcggtgcg gggggggccg
    17401 atccggagcc gggcgaccgt ccgcctcacg atccacgacg gcacgatcct ccgcgtcgag
    17461 gagctcgcgc cgccgccgcc cgggaccgtg gcgcgctgac ggggcgccgg tcgctctacg
    17521 acgaggcgag agcggggtcg agcggcgcgg gcggatgcgc cggggccggt gccggggccg
    17581 agggggaggc cgggacgaag tcgtgccggt tcgcccggac gacgcgaccg ccctcgtcgc
    17641 gcacgaccct ctcgtagtac gcgtccctct cgaacggctc gcggccggcg gagcggatga
    17701 ggcgctccat ctcgagccgc gagaggccgg cctcggtcgt cgcccccgcg gcgtgcgtga
    17761 tcgtctcctc cgtcacggtg ccgacgaggt cgtcggcccc gaagtggagc gcgaccgggg
    17821 cgaacttctc gccgatctgg acccagtagg ccttcacgtg gtcgacgttg tcgaggagga
    17881 gccgggcggc cgcgatgacc cgcaggtcga gcgtcccgct cgtcgccggg aggtgcgcga
    17941 acggcgtccc gtcggggtgg aacgcgagcg ggacgaagcc gaggaacccc ttcgtctcgt
    18001 cctgcaggcc gcggatccgg aggaggtggt cgacgcgctc ctcgtcggtc tcgatcgacc
    18061 catagagcat cgtgacgttc gtcttcacgc cctcgaggtg gcagaggcgc gcgatctcga
    18121 gccagcgctc gccgtcgatc ttgttcgtgc agatgacgtc gcggacccgc ttcgcgaaga
    18181 tctccgctcc gccgccgggg caggagtcca taccggcgtc cttcagctcg cgaatcgtct
    18241 cgacgaggtc cttcttcccg acgcgcgcga accagtcgag ctcgaccatc gtccaggcct
    18301 tgatgtggac gcccgggaag cgggcgcgga gcatgcggag gaggcccgga tagtagtcgt
    18361 aggggagcga agggtggagc ccgccgacga cgtggagctc ggtgatgccg tcgtgccatc
    18421 ccgcggccgc ctcgcggacg gcctcctcga tcgccatcgt gtaaccgccc gcgacgcccg
    18481 gcttgcgcgc ccaggcgcag aggccgcagt cgacccagca gaggttcgtc gggttcaggt
    18541 accggttgac gttgtagtag ccccggttcc cgtggcgcct ctcgcgctcg cggttcgcga
    18601 gggcgccgac gccgaggagg tccggcgagc ggaagaggac gaggccgtcc tcgaacgaga
    18661 gcctctcgcc ggcgagcgaa cgctcggcca cgcggcggag cgccgggtcg gcgagccgcc
    18721 cgaggagccg ttcggccgtc tccggctcgg gcgcggccgc cgtctcgaag gaacccgccg
    18781 gcgcgccggc gccggagggc gtcactcggg aacgtcctcc accgagtcgg ggcgcgagat
    18841 cagcggcggg agctgctgct cggccaccgc cctcgggtgg agctcgtcgt gccgtccctc
    18901 gtagcggtcg tagcaggctc ggcagaagcg gtagtcgggg cggaaggcgt agtcgacgac
    18961 cgtcccgacg ccgaggagct cgtcggcgct ctcgccgcac gagtcgcagg agccggtcat
    19021 cctgaggagc ttccagacgg gtccggacat ggggcggagt ctaccggacg ggcgggcggg
    19081 tccgctgggg cacggcgagc aggaggaaga ccgccgcgag cagggcgact cccgccccgg
    19141 cgaggaacgc cgtccgggag ccgaacctcg tccagaggag gccgaatccg gcgctcgccg
    19201 gaagcgccgc gagcccgacg gccccgtgga accagccgaa ggcacgccct cggctctcgg
    19261 gaggaacgag gtcggcgacg agcgcccgct cggccccctc cgagagcgcg gcgtggaccg
    19321 agtaggcggc gaggacgacg agcagggcga gcggagtctt cacgaacgcg aagagggtgt
    19381 agcaggccgc gtagagcgtc cacgcggcga agaggacccc tttcctcccg tgccggtccg
    19441 acaggccgcc gccccacgtg ccgagcgcgg cccggatcgc gttcgagagg gcccagacga
    19501 gagggagccc ggccgcggcg acgccgacct cgcgggcctt caggaggagg aacgtgtccg
    19561 acgaggccga gagcgtgaag agggcgaccg ccgcgacggc gcggcggaac gccggcggga
    19621 gcggggcggc gggcgcgcgg acgaccgcgg cagaggcggc cgccgagcgc ggagtctccc
    19681 ggacgcccag gacgaggacc aggagggcga gcgccgccgg cacgaaggag agcgcgagga
    19741 ccgtccggag ggggagcgcg aggaggccga gaagcgccgc ggcgacgagc ggcccggcga
    19801 ccgcgccggc gttgtccatg gcgcggtgga ggccgtaggc ggcgccccgg cggtcgggcg
    19861 gcgtcacctc ggcgatgaga gcgtcgcgcg gggccgagcg gacccccttt ccgatccggt
    19921 cgaggaagcg gacgaggagg acgtgcggcc agagagcggc gagggcgacg agaggacgca
    19981 cggcggagga gagcgcgtac ccggcgacga cgagcggctt gcgccgcgcg gctcgatccg
    20041 agagccggcc ggagacgacc ttcacgcccg cggccgtcgc ctcggcgacc ccctcgatca
    20101 cgccgagcgc cgcggccgga gctccgggga gggaggcgag gaggagcggc aggagcggga
    20161 agatcatctc ggacgaggcg tcggtcagga acgagaccgc gccgagggcc aggaccgtcc
    20221 gcgggaggcg ggagggagaa tgcgtgccgg tgctgctctc cacgacggcg ttcattctgc
    20281 gtgtcgacgc cctctccgag aaggacgtgg ccgcgacgct cctgacccgc gacgtcgggg
    20341 tcctccgcgc cgcgttccgg ggggcccgcc gccgcttccg ccacggggcg ctcctcgagc
    20401 tcctgacgga ggtctccgtc acgctctttc gaaaggaggg ggccgagctc gcccgcgggg
    20461 acgggctgga gctcgtgaag agcgcgttcg ggctcgcgtc gaagccggag accgcgatgc
    20521 tcctgccgta cgtcgcggag agcctctcga cgttcctccc ggaggccgag gcggcgcccg
    20581 aggcgtaccg gctcgcgcgc cacgtcctcg acgccctcga ggagggggcg ccgcccgcgc
    20641 tcgccgcccg ctatttcgag gcctggctcc tccgcctcgc cggcctcctg ccgggagggg
    20701 cggagtgcgc ctcctgcggc cgcagcctcg cgagcggcgg cgtccgcttc gacgcggagc
    20761 tgcccggcct cgtcggcgac gagtgcgccg cgcccggaac ggtgcccgtc tccgcggcgg
    20821 tcttgcgcct cctggcgagc gttcgccggt cgcggctgga cgacctcgtg cggcgggggg
    20881 ccggggatcc cgagtcgctc cgcgcgctcg acgacctgct gcgcgaggtg cgccgccgat
    20941 tcctcggcca cgagctgaag agccaccgct tcctcgacag cctctcgggc ggcccgtgac
    21001 gcgggcgccc ggccctgcta acctccgccg ctgccgcaat ttcgcgcgcc tcagaccgga
    21061 gcgaccatgg acttccagac gctgatcctg aagctgtccg aatactgggc ctcccgcggg
    21121 tgcatcctcg agcagccgta cgacacggag gtgggcgccg ggaccatgca cccggcgacg
    21181 ttcctccggg tcctcggccc cgagccgtgg cgctgcgcct acgtgcagcc gtcccggcgg
    21241 ccggccgacg ggcgctacgg cgagaacccg ttccgcctcg tgaagcacta ccagttccag
    21301 gtcgtgatca agccgtcgcc cgccgacatc caggacctct acgtcgacag cctccgggcc
    21361 atcggcatcg agccggccga gcacgacctg cgcttcgagg aggacaactg ggagtcgccg
    21421 acgctcggcg cctggggcgt cggctggcag gtcctcctgg acgggatgga gatcacccag
    21481 ttcacctact tccagcaggc cggcggcatc gacctcgcgc cgatcaccgc ggagatcacg
    21541 tacgggctcg agcgtttgac gatgttcctc acgcggacgc ggaacgtcat ggacatcgaa
    21601 tgggcgccgg gcgttccgta cggcgaggtg cggaagcagg acgagttcga ggtctcgaag
    21661 tacggcttcg aggtcgccga caccgacctg ctccggcagc tgttcgagag actcgaggcg
    21721 gagtccgggc gctgcctcgc ctccggcctc gtcgttcccg cctacgagac cgcgctgaaa
    21781 tgctcgcacg cgttcaacct cctcgatgcg cgcggggccg tctcggccac cgagcgcgtc
    21841 ggcgtcatca agcgcgtccg ggacctcgcg gtcggctgcg cgaaggcgtg ggtcgccgcg
    21901 cgcgagagcc tcgggcaccc gctcctcccg aaggagacgc cgatgtccga agcggccgaa
    21961 gccggcgacg gggaggcctg aaatgtcaga agctcccggg gggacggcta cgcgtacccc
    22021 ccggaccccc cgactcatcg cggagacgag gtaggttcct gatgtccgag cctcccgggg
    22081 ggacggctac gcgtaccccc cggacccccc aactcatcgc ggagacgagg tagatagccg
    22141 atgtccgagt tcttgctcga gatcctcgcc gaggagatcc ccgcgggcgt cctgtcgcag
    22201 gcacgcaacg acctcctgcg cgggctcgcc gacgccctcg ccgccgagcg gatcgacggg
    22261 acgttcttcg cccactccac gtcgcggcgc ctcgtcctct tctcgcggga cctccccgag
    22321 gcccaggagg accacgaggt ggaggcgatc ggcccgtccg tcaaggtcgc ctacgacnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca ggaggaccac gaggtggagg
    22501 cgatcggccc gtccgtcaag gtcgcctacg acgcggaggg gcgtccgacg cgcgccgccg
    22561 agggcttcgc ccgcggccag ggcgtcgcgg tcgaggcgct ccgcgtcgtc gcgacgccga
    22621 agggggagta cgtctcggtt cgcaaggtcg tcgaggggcg attcacctcg gagatcctcg
    22681 gcgaggtcgt cccgacggtc gtcgcgaaga tgtcgttccc ccggatgatg aggtggggcg
    22741 acggccgcga cgtctggatc cgcccggtcc attccgtcgt ctgcctcctc gacgggctcg
    22801 tcgtcccgat gacgctcttc gggatcgagt cgggccggac gacgttcggt caccggacgc
    22861 tcgcgccggg ccggatcgtc gtcgtcggcg tcgacgacta cctcgcgaag ctccgcgccg
    22921 cccacgtcga gccggactac ggcgtccgtc gccgaacgct cctcgagaag gccgaggccc
    22981 tcgcctccga ggctggctgc acgccggtgg aggacgtcgc gctcctcgac agccttgccc
    23041 acctcgtgga gtggcccggc ctggtccgcg gctcgttcga ggccgggtac ctcgagcttc
    23101 ccgaggagat cctcgtcacg gcgatgcgcg agcaccagaa ggtcctgccg acgcgggacg
    23161 ccgcaggagc gctcgcgccg cacttcctcg ccgtcgccga ccacgtcggc gacccgatgg
    23221 ggcacatcgt ccgaggcaac gagtgggtgc tgaacgcccg gttcgccgac gcccgcttct
    23281 tctgggacga agacggacgg gtccgcctcg aggagcgggt cccccgcctc gctcgccttt
    23341 ccttccagga gaagctcggc gacacgctcc ggaaggccga gcgggtcgag gcgctctccg
    23401 ctcgcctcgc cgccgacctc ggcctcgagg cttctgcgga cgaagccgca cgtgcggcac
    23461 gcctggcgcg ggccgatctc gtcaccgaca tggtgcgcga gttcccggat ctccaggggg
    23521 tcgtcggggg tctctacgca cgccgggacg gcgagagcga gacggtctgc cgcgccgtct
    23581 acgaccacta ccgtccggcc ggcgtcttcg acgccctccc gggggagccc gtcggccgcg
    23641 tcgtcgcgct cgccgaccgg ctcgacacgc tcgccgggtt cttcgcgctc ggcctcgtcc
    23701 cgaccggctc gcgcgacccg tacgccctcc ggcgggcggc gctcgccgtc gtccggatcc
    23761 tcctcgaggg cgggcaccgg ctggacctcc cggtcgcgat caccgccgcc cgccgcctcc
    23821 acgaggtgtc gggcgcctcg gaggaggagg tcgtctcgac gctgcacccg ttcctcctcg
    23881 agcgcctccg cttcctgctc gagcgacgcg gtcaccggca cgacgagatc gaggccgtcc
    23941 tcacgacgtc gtgccccgac gtgaccgacg cggccgagcg cgtcgccgcc gtctcgcgcg
    24001 tgaggcggca gcccgacttc ggagcgctcg ccgcctcgtt caagcgcgtg cagaacatcc
    24061 tcgtccaggc gggcgtcgcg tcgggaggcg agatcgaccc ggcactcatg agcgacgatg
    24121 cggagaggca gctcgcgggt gacttcttcc aggcccgcgg gatcctcgac gagctgatcg
    24181 cctcgcgccg gtacgacgag gcgctctcgg tcatggcctc tctcgggccg gtcctcgacc
    24241 gcttcttcgt cgacgtcatg gtgctggtgg aggacgaggc cgtgaggcga aaccggctct
    24301 cgctcctcgc cgcgatgcgc gaccagttct tccgcgtcgc gcgcctgagc gagatccagg
    24361 agtagggtcc cggcgcccgg ctccgccgcc ttcccaccgg ggagcggcgg agccggaggg
    24421 cgccggccct gctgccgaag cgcgcgatcg cagtatcctc gcggcccgaa ggaggacgac
    24481 ccatgaggac cacccggcga gccgtcgccc tgtctgccgt attcgcgttc gctctttccg
    24541 ccgcttcggc ggaggcgaga gagctggcgg gcgcctcgat gcccgacacc ctctcggtgg
    24601 cggggaagac gctgaagctg aacggaatgg ggcttcgaaa gaaggccgtg ttcaaggtct
    24661 acgtcggtgg cctctacctc gagacgccct cctccgacgc ggccgcgatc ctcgccgccg
    24721 acgcgccgaa ggcggtccgg atgcacttcc tccggagcgt cgaccgcggg aagctcgtgg
    24781 agacgttccg ggaggggttc gccgcgaacg ccggggcgaa ggcggaggcc cagaaggcgg
    24841 ggatcgagaa gctcctcgcg accgtgacgg acgtgaagga cggctcggtg acgacgtacg
    24901 tctacgcgcc gggcgccggg accgtcgtca accgcgacgg gaaggacgtc gcgacgatcg
    24961 aagggaagga gttcgccgag gtcctcttct cgctctggct cgggccgaag cctccgtcgg
    25021 aggatctccg gagcggaatg ctcgggcgct gaaggtcccg cggaggcgaa tcggagcggg
    25081 gcgggtacac tcccgccccg tcatgaatct acccgagctc tcgtcgctcc tctccgagaa
    25141 gaaattcgac gtcgtcgaga acgccgtcaa ggacgccctc ctcgacgccg aggagaacgc
    25201 ggagctcctc gcggcggcgt tccgcggcct cgcccgcgcg tccggccaga aggggcgcct
    25261 ccagggcctc tccacgctcg ccgacgcgac gctccgcggc ccgggcacgt ctctcgcggc
    25321 ggcccgcctc cgctggagcc tgttgaagga ggccgtccgg gccggcgcca cgccctcctc
    25381 gccggagggc ttccacaagc tcttcgagga ggcgatccgg gcggcgtacc ccgagtcggc
    25441 gtccctgacg acgctcctcg gccggttccg tttcctcgag tcgaaggagc cggcggacgg
    25501 cctcgcgcgc gtcgagcgcg ccgagaagtg gctccccttc gaggtggggc gctgcttctt
    25561 catggcggta cggggcgccg ggaaggtcgt cgagacgaac ttcgccctcg acgccgtccg
    25621 ggtcgacttc gagaagtcgg tcggggtctc cattccgatc ggcgtcgcgg cgaagagcct
    25681 cacgccgctc cccgaggggc acttcctcca cgagaagctg accgcccgcg aggcgctggc
    25741 cgagagcgtc ctttccgacc cgtcggaggg gcttcggagg ctcctcgact cgttcgggag
    25801 ggcgcttccc gtcgccgagg tgaaggaggc gctggccggc ctcgtccccg aggagcgctg
    25861 gacctcgtgg tggaccgcgg cgcgcaagag cccgcacgtc gtcctccacg gctcgggcaa
    25921 gaacgcctcc gtcgaatgga cgcggacctc gcaggcggcc gacgccgcga tcctcgcgaa
    25981 gttcgggaag gcgcacacgc ccgaggcccg tctcgacttc ttccggaaga acgcgaagcg
    26041 cgatccggcg ctcgcggcgc agatggggac gaagctcgtc gaggacgcgc gggcgctccg
    26101 cgacgcgaac ccgccgctcg cgtacgagat cgcggtcgcg atcgagaagg tcgagggcgt
    26161 cgcctccggg ttcgcgcccg aggacctcgt gcccgaggcg ccgctgccga tgctcggccg
    26221 gatcggggac cgcctcgcgc gggagcgctt cctcgaaggc ctcctcgcct cgaggccgga
    26281 ggacggcgcc cgcctcgtgg cggagtggtt cttccgggag gaggacgccc ggactctcga
    26341 cctgatcgac cggcggctcg ccgaggtcga ccccgcgacg cgggaggcga cgctcgagaa
    26401 gctcctcaag tctccccgga gcgggccgaa ggcgttcctc tggttcggcc tgcggtccgc
    26461 gcaggacgac gcgttccgcg cccgcctcac gccgacggtg ctcggccgcc tcctcgacgc
    26521 cgcgtcgtgg gaggacctcg ggggaggccg cgcgaagctc cgcgagctct tcgaccggac
    26581 cggggtcgcg gcggcctggc tcgtcaagca ggcgacgctc gaggaggccc agcagttcct
    26641 ccaggcgctc gaccggcaca acgagctcga gccgcaccgc cgcgcggcgc tcttcgcggc
    26701 ggccgagatg cggttccccg acctccgcaa gggaggggac gacagcttct tcgtcacgga
    26761 cgaggcgctc gaggggaaga agaaggagct cgagaggctc gtggaggtcg acatcccgga
    26821 gaacacgaag gggctcgcca tcgcccgcgc cgagggggac ctctccgaga acttcgagta
    26881 ccaggcccgc aagcagcggc agcaggacct cggagtccgc gtggcgcgcc tccaggagga
    26941 gctgcgcaag gcgcgcgtcc tcgacccggc gacggtcgac acgacggagg tgaggcccgg
    27001 gacgcgggtg acgctccggc tcctcggcgg gacccgcgcg gtgacgctcc tcggcccgtg
    27061 ggactcgcgg cccgaggccg acgtctactc ctacctcgcc gacgcctcca aggggctcct
    27121 cggcaggaag gtcggggacc gcgtctcctt cctcggagac gaagggctca tcgaggcgat
    27181 cgaggtctgg aagtaggact ggcgcggccc cgtcgccgac ctcgaggggc gggccctcga
    27241 ggtccggcgg cggttcgagg agggcgttcg ctacactccc cgggtgaggt cccgaatcct
    27301 cgcccgagcc cgccggatct ggagggcgat cccccggtgg gcgctctggg gtgcggccgg
    27361 cctcgtcctc gccctcgccg tcgtctcggc gttcgtcgtc gcctactacg cccgcgtcgt
    27421 gtcgcggacg atggacggga ggcgctggaa cctcccgacg cggctctatt ccgacgcgtg
    27481 ggtcctccgt cccggcgacg cgctcgggcc ggacgacgtg gagcggcggc tcgcgcggct
    27541 ccggtacgcg cccgttgccg agcttcccgt ctccggcggc cgctacctcc gcacgcggag
    27601 ccggatcgtc gtcggcgtca acccgcgcga gacggcctac ggccgctccc cggggctcgc
    27661 cgcccggatc gacttctcgg gccgccggat ctcggccctc cggcgcgagc cggacggggc
    27721 gccgatcccc tacgtcgtct tcgagccgga ggtcgtcggc tccgtcttcg accgaaagat
    27781 ggaggaccgg acgctcgtga ggctcgagag ggtcccgaag gtcgtcgtcg acgcgatcgt
    27841 cacgacggag gaccgggact tcttcacgca cgcggggctc gacccgacgc ggctggccgg
    27901 cgcgctcctt caggggatcg cgcgcgggcg caaggtccgc gggacgagca cgctcacgca
    27961 gcagctcgtc aagaacctct tcctcacgcc cgagcggacg ctccggcgaa aggccgtgga
    28021 ggcgctcctc gccgtcatcc tcgacgcgaa gtacggcaag gaggagatcc tcgaggcgta
    28081 cctcaacgag atctacctcg ggcagcg
//
LOCUS       DAOONL010000037        27970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1501_length_27970_cov_18.855669, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000037 DAOONL010000000
VERSION     DAOONL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27970
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_27970_cov_18.855669"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLP50_07660"
     CDS             <1..482
                     /locus_tag="PLP50_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51464.1"
                     /translation="FPLAWRLFSDEPTAEEMASVLDEALARARRLHPGVSLSHFATDK
                     GACFTAETFQAAVARHGMKARFGAVGRHGSIALIERLWLGPKGLLHLRLDRCLLRDDL
                     ESRIGLGLFYDSVLKPHQGLGGATPAEVYFQSGPARDHALPPPREGDPIPPPAFAVR"
     gene            complement(734..1324)
                     /locus_tag="PLP50_07665"
     CDS             complement(734..1324)
                     /locus_tag="PLP50_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPA51465.1"
                     /translation="MTAPSREIDRRRFVADLIEACPDVLAIAGLGAPAYDLFAAGDRD
                     TSFYLWGGMGGAAAMGLGLALAQPERSVLVLTGDGEQLMGLGALATIGARRPPNLTVV
                     VLDNGRYGETGMQETHTSLGTSLSGVAVACGLAWVETVSSAAELPRLAQRIRERAGCG
                     LAQVVVRAETMPRALPPRDGLLLKRRFRAALDARRP"
     gene            complement(1321..1863)
                     /locus_tag="PLP50_07670"
     CDS             complement(1321..1863)
                     /locus_tag="PLP50_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPA51466.1"
                     /translation="MTSVSDPVPAPWQEEVFSALKGAGVRQVAWVPDAGHAHVIDRVL
                     ADPGLLGIPLTTEEEGVALACGAALGGDRAVLLMQSSGVGNCVNMLSVVSTCRFPFLA
                     LVTMRGEWEERNEWQEPMGRATRTVLEAMGVTVLRAEEPGAVAGVVREALVAAFEREE
                     AVAVLLAQRLVGRKDWGDGK"
     gene            complement(1860..3062)
                     /locus_tag="PLP50_07675"
     CDS             complement(1860..3062)
                     /locus_tag="PLP50_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51467.1"
                     /translation="MTFADVLRLALGSVTAHRLRSALTVLGIVIGIASVVLLTSLGEG
                     TRQAILSEFSQFGTNLLAVTSGKIETHGIAGAVGGTTRPLTLDDAEALRAVPGIEKVV
                     PVVFGTARVSSGERARDTFVYGATSDGPEVWRFQVRLGRFLPETELDRATPVAVLGPK
                     LKRELFGAENAIGRRIRVGGHRFVVIGVMATVGQFVGFTLDDAVYVPVASAQQLFDQA
                     QVMEIDATFTPGLPPETVVAAVKRVLKARHGGEEDFTVMTQGEMLESFGRVFAIVSVV
                     VGAIGAISLLVGAIGILTMMWIAVGERTAEIGLLKALGATRGDVRNVFLAEAAILSGA
                     GGLAGVLLAVTLGSLVWLALPAVPFATPPEYAGAALATALVVGLVAGVAPAGRAAALD
                     PVENLRAE"
     gene            complement(3067..4269)
                     /locus_tag="PLP50_07680"
     CDS             complement(3067..4269)
                     /locus_tag="PLP50_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51468.1"
                     /translation="MRGGDLLAFAGGAFRGHRLRTSLSLLGVAIGVAAVILLTSLGEG
                     ARRYVTGEFALLGSNLVIVLPGKAETTGVVPVGGVPHDLTLEDVEALRRRVSLLVSVA
                     PLSVGGLTARFGERSRDLTVAGVTSDWKDVRRLALREGVFIPPGNPDRAPRVCVVGAT
                     VASELFPGRSPVGELLRLGDEKFRVTGVLVPRGVSVGLDLDEFVLVPIAHHLRMFDRR
                     SVFRVLCEARTSEDLEAAKAGILAVLKDRHDGEEDVTVLTQDAVMKTFGKVLGILTAA
                     LAGIAAVSLTVAGVGIMNVMLVSVSERTREVGLLKALGASGGQVLRIFLVEAAILSSA
                     GGVLGLLAGGLGTLAFRALYPDFPLAPPAWAVAAALGVSVSVGLVFGVVPARRAARLD
                     PVVALARR"
     gene            complement(4266..4940)
                     /locus_tag="PLP50_07685"
     CDS             complement(4266..4940)
                     /locus_tag="PLP50_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51469.1"
                     /translation="MIELTGVSRTWEVGGRPVHALRDVTLSIGAGEYVSVMGPSGSGK
                     STLLSLLGGLDRPTAGSYRFEGREVASLPDEELSRLRRERIGFVFQSFHLVPRMTALE
                     NVALPLLLAGVAPKERTARAAEALASVGLSPRLDHRPDQLSGGEKQRVCLARAVVTRP
                     GLVLADEPTGNLDSASGAEIVALLERLHSSGITLLVVTHDAGIGGRARRRVRLADGSV
                     VGDEAA"
     gene            complement(4937..6085)
                     /locus_tag="PLP50_07690"
     CDS             complement(4937..6085)
                     /locus_tag="PLP50_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51470.1"
                     /translation="MKQAWLRRGGVLLGIVAAVLLLRVTLFRPDPLPVTVVAVAKGAV
                     EETVAGSRAGTVKSRRRATLSPEVGGRVERLAVREGDRVRKGDLLVRIAAEDVRAQVA
                     LAEKSLVVAGAAEVEACRAAEQARRDLARSRSLAKDEVLSVGLLEKAETEAGMTAAAC
                     DAARARVGQARASLDVARVALEKTVLKAPFDGVVAEMSTEEGEWVTPSPPGLPIPPVV
                     ELFDPDDVYVSAPLDEVDLGRVKKDAEVRVTIDAYPGRTFAGRVRRIADYVLDQREQN
                     RTFEVEVALDDAAFARTLLPGASADAVVVLARKEDVLRVPSYAILPGGRALVLSKGRL
                     AEAKVETGLKGAEWAEVVSGLSAGDLVVTSLDRPEVKEGARARAEEAR"
     gene            6170..7213
                     /locus_tag="PLP50_07695"
     CDS             6170..7213
                     /locus_tag="PLP50_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPA51471.1"
                     /translation="MNRAPRLAVASAFLALAACSRSAPEPRVGREERKALAAQAGSTL
                     GVLPERMPGAEADSPELVALGRSLWFERRLSVTGTQSCNDCHRLDGQNPTGADVAPTS
                     NGARGTKGPRNSPSVKNAGYHVAQFWDGRARTLEEQAEGPILNPVEMAMPDAAAVEAV
                     LRGAAEYGAPFATAFPGVPQPITLPNTARALAAFERTLKTRDRLDDFLRGDLEALSHR
                     EAAGLKLFLTTGCTTCHNSPTIGANQFQVLGLVKEWETKDEGRFAVTKNEEDRRKFKV
                     PSLRNAVATGPYFHDGSVDRLEVAVKKMAWHQLGKELTDDEIASIVAFLGALADRSGP
                     ILPPAPSTKEADR"
     gene            7210..7542
                     /locus_tag="PLP50_07700"
     CDS             7210..7542
                     /locus_tag="PLP50_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51472.1"
                     /translation="MNHESLVKDAAARSGLPEATVEKVVAALADAVHHAHATPDELLH
                     ALLGADEPLHAHPVDPRDAVVVADLLERARAHPLGLDYLKGGHLGSVAVTFETHAFTV
                     LAARERLR"
     gene            7545..8561
                     /locus_tag="PLP50_07705"
     CDS             7545..8561
                     /locus_tag="PLP50_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HPA51473.1"
                     /translation="MKSWNLPKRVDLADITVRDGFQHEERWIPTEAKLWVLEELVLAG
                     YRRLEVTNLGNPKGMPQFRDADALLKGIRGSARVSKQIGEVELTAVTIREKAVDRAIA
                     AKSEGWGPDRILMMVSTSASHMKKNSGLDHAGYWAEAERCIRKAHDAGIRVNGTVSTI
                     WGCPIEGPTKLEDAVAFTKRFLDIGADDIEHADHDGSAPPDRVYAYYSIVLDAIPDVT
                     KHLAHFHVTRGWGLANVLAALQAGIVRFESTLGGIGGQPANFFEGAPVSGTGRYYYQD
                     PNNVGLVCTEDLVVMLDEMGLETGLDVDRVLATGRMVERIVGRRLRSETVKSGRIPKR
                     PTGF"
     gene            8934..9590
                     /locus_tag="PLP50_07710"
     CDS             8934..9590
                     /locus_tag="PLP50_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPA51474.1"
                     /translation="MAQLFPKSANSLAPASLAALVLVAGGAVGSLLALDYAGFNQRRG
                     ETVEQKVPFSHEHHVAGLGIDCRYCHTSVETSAVAGVPPTSTCYNCHKTVWNEAPMLE
                     PVRSSVKTGQPLEWTRVHDLPDFAYFNHSIHVAKGIGCATCHGPVHEMRLMRSAQPLQ
                     MRWCLDCHRNPEKFIRPKEEIYNMTWKAKDQETLGRELVKAYGVRPPAEITSCGTCHR
                     "
     gene            9614..12664
                     /locus_tag="PLP50_07715"
     CDS             9614..12664
                     /locus_tag="PLP50_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HPA51475.1"
                     /translation="MTPSSLHPGPADDTPARFPIDLAAIRERLDRRQGPAFWRGLEEL
                     ADDAGFREFLAKEYPRHASDWDESLDRGFSRRRFLELGMASMALAGMTACTRQPVEKI
                     VPYVKQPEEIVPGVPLFFATAATIGGFAQPLLAESHEGRPTKVEGNPEHPASLGGATA
                     LAQASVLGLYDPDRLKVPTKLGLLDTWPRLVSELQAAAKAQKQVEGAGLRILTQTVTS
                     PTLGAQLAALLAAYPKAKWHQWEPAGRDAVRAGARLAFGRAVETRYDLGAADVVVALD
                     SDLLASGPDSVRNAKAFSARRRLTGGKTETSRFYAVEVSPTPTGTLADHRLPLRASLL
                     PAFAQALGAALGLPGFAAPAGLDESAARFAATAANDLRAAAGRSVVVAGDFAPPEVHA
                     AAHAINEALGSLGKTVFLTEPVEAAPIDQLASLRELAEDVKAGSVELLLILGGNPVYD
                     APSDLAFGEALLKAGLTVALASHPDETTRYCHWAVPMAHDLEAWSDARSLDGTAALVQ
                     PLIEPLYEGKSAHELLSVLLDEVPRKGYDVVREAWKAARPAEADFEAFWRKALHDGVV
                     PGSAAPAVAAPVRLAEVAAALAAASAVKPAGLEAVFRPDPSLLDGRFANNGWLQELPK
                     PLSKLTWDNAAVVSPATAQELGVTNEDVVKVEAGGRSAELPVLIQPGQAAGVVTLHLG
                     FGRKHAGRVGNGVGADVNPLRTTAAPWLVPAVTVTKTGGRHPLAHTQQHFRMEGRDIV
                     RTMTLPEYRKDPAKAKGHGLPPKEASLFQPGWEYKENAWGLSVDLGSCFGCNACVVAC
                     QSENNVPVVGKEQVSKNREMHWIRVDQYYEGSDLENPAVHHQPLMCQHCEMAPCEVVC
                     PVAATVHGAEGLNEMAYNRCVGTRYCSNNCPYKVRRFNFLEYNGQREEEPVLKMLANP
                     DVTVRSRGVMEKCTYCIQRINRARIAAEREGRAIRDGEIKTACQQTCPADAIVFGNIN
                     DPAAGVSKLRSEPRTYWLLDELNTRPRTTYLARVTNPSPDVEGA"
     gene            12666..14066
                     /gene="nrfD"
                     /locus_tag="PLP50_07720"
     CDS             12666..14066
                     /gene="nrfD"
                     /locus_tag="PLP50_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPA51476.1"
                     /translation="MSTAYDDVRGADPIAQPPVLAPGHTPASVTERISEAVTTKLTKT
                     PKWWLGGFAVGFALLNVFLFAVTNLFMTGIGVWGNNIPVGWGWDIICFVWWIGIGHAG
                     TLISAILLLLRQSWRNSINRFAEAMTVFAVACAGMYPILHLGRPWLPHWLLPLPGTLG
                     MWPNFRSPLIWDVFAVSTYATISIVFWYVGMLPDLATLRDHAKAKFARFAYGMAALGW
                     RNSARHWANYEVASLLLAGLSTPLVLSVHSIVSFDFSVGIVPGWHTTVFPPYFVAGAV
                     FAGFAMVLTLVLPLRPLFKLHDFITERHLQNMAKVMLTTGLIVAYGYVLEIWAAFYSG
                     NEFEIFMVLNRFTGPYATQYWLLILLNIAIPQLLWLKGVRSNVWALWAISMCINVGMW
                     LERFVIIVVSLHRDYVPSNWAMYSGTKFDVMTFVGTMGLFLTLLLLFIRFLPALNMFE
                     MRVLTPEATKAHGEEK"
     gene            14071..14622
                     /locus_tag="PLP50_07725"
     CDS             14071..14622
                     /locus_tag="PLP50_07725"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HPA51477.1"
                     /translation="MHGTHPEPSLHGVLAEFRTPDELLAAIEAAKSAGYTKLDAYTPY
                     PIEKVLDALDLHHSKVPLLTLGAAIFGAVAVFSFAYWASAIDYPLNIGGRPLNSWPAF
                     IPATFEGAVFHGGLAAALGMLVLNGLPRPHHPVFEVKRFVEHASKDGYFLLVEADDPR
                     FDVSAVRRLLEGLAPVEVNDLEN"
     gene            14612..15244
                     /locus_tag="PLP50_07730"
     CDS             14612..15244
                     /locus_tag="PLP50_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPA51478.1"
                     /translation="MKTDLPPGRRLGVAALAVAALLGAGCRQGMYDQPKAKPLGRSDF
                     FRDGLSSRVPPAGTVARGWLREDRVLHTGIGPDGKFVSELPGAVPFDKALLLRGRQRY
                     DVFCSPCHGRQGNGEGMIVQRGFKQPVSFHVDRLRAQPLGYYFDVMTNGFNEMSSYAS
                     QVPVKDRWAIAAYVRALQLSRNAPASELSAADLEALEKAAASAAAPEVHP"
     gene            15241..16422
                     /locus_tag="PLP50_07735"
     CDS             15241..16422
                     /locus_tag="PLP50_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51479.1"
                     /translation="MNQGSNPLPEGVEKLGRIGLVAGLVGLALLGAGYATDRDQLFRS
                     YLTGYMWMLGLAAGALGVMLLHHVTRGAWGVVIRRILEAAASPLTLGFAAVAFLPIAL
                     GSHSLYEWTHTDVVAGDAILTHKKPWLNEPAFLGRSAAYLVLWIVLAAVFARLSARQD
                     ETGDRRIALSMQRWGAFGVVAFILSMTFAAFDWMMSLTPHWFSTIYGLYVIIGQAVAA
                     MALVALAGLLLGEGPAPALPLQARHLHDHGKLLFAFVCVWAYFGYSQFIIIWSGNLPE
                     ETSFYASRLNGAWKATTVVLVLLHYALPFALLLSRNRKRSAKALAPVAVLLLFARWLD
                     LHWLVAPAFSANAFTLHWLDLAAPLALGGIWLRLFTLGLKARPLLPANEPFLKEALAH
                     E"
     gene            16415..16846
                     /locus_tag="PLP50_07740"
     CDS             16415..16846
                     /locus_tag="PLP50_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51480.1"
                     /translation="MSDHPRPQHAPAEFDSEIHVKAIFGVLAGLALLIALAFGGMIAF
                     SGYLKSRSIARDPEPLPLAEANAPRPRPRAALQADPTADMTKFAREEEAAVSSYAWVD
                     RAAGVAQIPVERALEIVAERGLPVPPPLPEAGPAPAAGGSK"
     gene            16843..17667
                     /locus_tag="PLP50_07745"
     CDS             16843..17667
                     /locus_tag="PLP50_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPA51481.1"
                     /translation="MSRTVRLAGLGLLAVLVGSPALAQPMMPSPTREVGWEQNMGAQL
                     PLDAKFTDENGKAVRLGDYFGKRPVLLSLAYYECPMLCGIALEGLARSLKGFTLTPGA
                     DFEVVTLSFSPTEGPELAKAKKSNLLDLYGRKAEGQAGWHFLTGSDAEIRRVTEAVGF
                     RYRWDELQKQYAHATGIVVVTPEGVISRYFFGVEYAPKELRLGLSEASEGKVGGVAAQ
                     LLLLCFEYNPALGKYTAETMTALRIAGGFLVVAFGVFLAVSLRRERRSRAHLGANA"
     gene            17670..18650
                     /gene="coxB"
                     /locus_tag="PLP50_07750"
     CDS             17670..18650
                     /gene="coxB"
                     /locus_tag="PLP50_07750"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPA51482.1"
                     /translation="MLPNAPVFPPVSSTLASKVDTLFFFTLGVSVLFSVAIVATIAAF
                     IVRYRRRRADETGQEIKGNTLLLELAWSIVPLGLVMVSFFWGASIFFESARPPADAWS
                     FHVYGKQWMWKVEHPEGKREINEFHVPVNQAVKLTMTSEDVIHNFSVPAFRLKMDVLP
                     GRYTTAWFRATKTGTYHIFCDQYCGVDHSKMVGKIHVMEPQDYKRWLVSGASAGGAVA
                     SGEELFVAKACNTCHRPDSSARAPVLDGLFGKKVALVDGRTVTVDESYVRESILSPAT
                     KVVQGYQPIMPTFQGQISEEEVVQLIKYIKDLPPAGAPGAPAAAPAGGKP"
     gene            18680..20287
                     /gene="ctaD"
                     /locus_tag="PLP50_07755"
     CDS             18680..20287
                     /gene="ctaD"
                     /locus_tag="PLP50_07755"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPA51483.1"
                     /translation="MPSYLEAAHGPKSWLLTRDHKRIGILYLVAISVFFVLGGLMAVL
                     IRAELATPQGDMFSSDVYNRLFTMHGILMIFFFLIPSIPAVLGNFLLPVMIGAKDLAF
                     PKLNLTSWYLYMIGGLMTFGSLLTGGIDTGWTFYTPMSTTFAQGQVLVTAVGIFVAGF
                     SSILTGLNFIVTIHRMRAPGMTWFRLPLFVWAHYATSIIQILGTPVVAIAILLVGAER
                     LFHIGVFDPKYGGDPLLFQHLFWFYSHPAVYIMILPAMGVMSELVTAFSRKRVFGYHF
                     VAFSSLFIAVIGFLVWGHHMYVSGQSVVAGAIFSFLTMLVAIPSAVKVFNWTATLYKG
                     SISLQTPMLYALGFIGVFLIGGLTGVMLGTLGIDIHVHDTYFIVAHFHYVMVGSAIMG
                     YLGGLHFWWPKITGRMYDEFWSKVGALLIFVGFNTTFFPQFILGYLGMPRRYHMYPAE
                     FQTWNVLSSAGATILGVGYLIPVIYLLWSLKFGAKAPANPWGAKGLEWQTASPPPVEN
                     FATPPVVTEEAYAYETSVPAKEVTVGI"
     gene            20277..20933
                     /locus_tag="PLP50_07760"
     CDS             20277..20933
                     /locus_tag="PLP50_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HPA51484.1"
                     /translation="MASEARPAPLAHHFDDYDQQRESATLGMWLFLAQEVMFFGGLFL
                     AYTVYRWKYPAWFALGSNQLDVPLGLANTIVLIGSSLTMVLAVRAAQLGKARQIAGWI
                     GATMALGTAFLVVKGFEYHHKWVHHLIPGAGFHIEGTTDPAAQTFFVLYFCMTGLHAL
                     HMIVGLGLLAFLVPKALRGDFSAENHNWVEGVGLYWHFVDVIWIFLFPLLYLLGRHHA
                     "
     gene            20950..21279
                     /locus_tag="PLP50_07765"
     CDS             20950..21279
                     /locus_tag="PLP50_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HPA51485.1"
                     /translation="MSTVHVTPVRTYLLVFFGLMALTFLTVGVAHVNIADHLPGQVTD
                     AINDAVAMAIAVTKATLVVLFFMHVWHSARLNKIVVWASLFFILILFAFSLADYFSRG
                     WLGVPGK"
     gene            complement(21366..22844)
                     /locus_tag="PLP50_07770"
     CDS             complement(21366..22844)
                     /locus_tag="PLP50_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA51486.1"
                     /translation="MTEGRVARERRALLPLLAGAFLIVPGALRAAGPTEAEVASAASR
                     ITAASAFTTVEVLAAPGMAGRLSGTEGYRKAADHVVAELRKAGLRPPADLPDYRQAFT
                     HGLAGVEAASLTLLPAEGEKDGTPYEAEVLKDFSPMVNGGSGDVTAEVVFVGYGFDAA
                     SEGRDDYAGVDVRGKVVLVLRGEPEGREWKDHRSTVARTKAAARKGAAGLLLIDSPVT
                     STSVGISRDLPEAMVSEAFADRLLAGKKLTVAELRKVLSKGGLASFATGSRLRFQVKG
                     RPWREVTTHNVVALLPGSDPSVKGEYVVVGAHLDHIGEWPRLNPGADDNASGSASLLE
                     VARAAATLKVRPRRTIAFVFFAAEELGLLGATHFASHPPKGLETCVAVLNMDMLGAGN
                     GLYVAGGENFPEIKAAMERARDRLAPGFNLRSGKIRGEGRADHAPFFEKGIHAVSLFA
                     MGGDHHGYHTPEDTVYFVTPKTMESGARTVFGAAIALADAKP"
     gene            complement(22841..24019)
                     /locus_tag="PLP50_07775"
     CDS             complement(22841..24019)
                     /locus_tag="PLP50_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA51487.1"
                     /translation="MKDRLSRRDLLALAATSGAVLALPRWARAAEAAPAKKKVLILGG
                     TRFLGVAIVEAALAKGWELTLFNRGKSNPGLFKGRPLEEIHGDRNVADDVKKLAGRKW
                     DAVIDTSGYFPRQVRSAAEVLSGNVGQYVFISSISVYDAPMKAGMDETAAVARLAAGT
                     DVDAIKDITGGNYGALKLLCEEAAEKAMPGKALNVRPGYIVGDRDGSDRFTYWPVRVR
                     KGGEVLVAGKPADPLQFIDVRDLGDWIVRMVESGTNGVFNATGPKERLTMKAFLETCR
                     KETKSNATFTWAPEAKLKELGLTPEADFPIWVSTEGPEAGIGDVSIARALKAGLTFRP
                     LPETIASTLHWWDSLPEERRAKARAGLAAEKEAEVLTALKGKAPAAAAPAAPPSEVKK
                     "
     gene            complement(24131..24610)
                     /locus_tag="PLP50_07780"
     CDS             complement(24131..24610)
                     /locus_tag="PLP50_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPA51488.1"
                     /translation="MARLTGVFLDTSVLLGGTINVGPSSRPAQAVMDAVAEGRLRGAR
                     TAWHCCLEYYAVATRLPEELRLAPADALRLLEEEVLGRLDVVDLPAVARLPFLRSAVA
                     ERVAGGRLYDAHIAEIARRSGSDVIVTDNVRHFRGRPESPVRVLSAAELTAELRAGS"
     gene            complement(24598..24855)
                     /locus_tag="PLP50_07785"
     CDS             complement(24598..24855)
                     /locus_tag="PLP50_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPA51489.1"
                     /translation="MKASIDKAGRIVIPASVRARAGLTPGTEVEILVDDVSVRLVRKV
                     PGPKLVRRGRRLVAQPTVRGRALPTVDVAALVEEERNRWPG"
     gene            complement(24921..>27970)
                     /locus_tag="PLP50_07790"
     CDS             complement(24921..>27970)
                     /locus_tag="PLP50_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPA51490.1"
                     /translation="LERFRGGKLLSRTLLTAGKDPTVVEFPVTEADRGGFGVTLTALR
                     DHQWLSQTASVFVPWDDRELKLEFATFRDRIRPGTRETWRVKVTSPSGKPVEAGAAEL
                     LAYMYDRSLDVFAPHHPPSILSLYPSRTGTAWTRATLGAGRAQWVRGSFAPTPSGPSF
                     SPDRLLELDRYGIGGPGARGRRFAMRGGMGVAMVAESALERDGVPAPAPAAAPQMATS
                     VDMKKEDAGANEARTEAGVAPAAEAPALRSNFAETAFFTPQLLTGADGSASIEFTVPD
                     SVTSWNVWLHAVTKDLRGASLKRETKSVKELMVRPYVPRFLREGDRAELKVVVNNASE
                     GTLAGSLVLQVLDPETNEDLTARFGLPVADARRAVSVPAGGGANETFHLIAPPGVRTV
                     AFKVTATSGNLSDGELRPLPVLPSRMHLVQSRFVTLREGQRKEMRFEDLAKGGDPTLL
                     NEQMVVTVDAQLFYGVLEALPYLVNYPYECTEQTLNRFVSTGIVTSMFEKYPSVAAMA
                     KEMSKRDVRLETWDAADPNRKMTLEESPWLVDAKGGKDAGEGLAKVLDPRVAKAEREA
                     ALAKLAKAQTSIGGFPWWPGGPPSPYMTVYILHGLAKATEFGVDVPKEMVQRAWGYVA
                     KHYRDDLLSLMRKDCCWELLTFVNYVASCFPDPSWSENGLTEAERKEILDFSFKHWKE
                     HSPYLKGYLALTLKRANRMKDAQLVFASVMDSAKTTEELGTFWAQEDRSWLWYNDTIE
                     THAFALRTLTELTPKDPRRDGLVQWLFLNKKLNQWKSTRATAEVLYSLVHYLKAEGAL
                     GGRETMKVAVGGRETSFTFEPDRYTGRKNQVVIPGPQVDPKTTSTIAVEKGGKGFAFA
                     SAAWHFSTEKLPEADRGDFFNVSRRYFLRENTGKEWVLKPIADGTVLKPGDQVEVQIS
                     LRTKHEAEYVHLRDPRGAGFEPETTVSRYKWDLGIAWYEEVRDSGTNFFFEQLPVGEY
                     TFKYRLRANMAGTFRVGPATVQSMYAPEFNAYSSGAVLTVKGE"
BASE COUNT         4006 a        10606 c         9219 g         4139 t
ORIGIN      
        1 gcttccccct cgcctggcgc ctgttctccg acgagccgac ggccgaggag atggcctccg
       61 tcctcgacga agctctcgcc cgcgccagga gacttcaccc cggcgtttct ctctcgcact
      121 tcgcgaccga caagggtgcc tgcttcaccg ccgagacctt ccaggcggcc gttgcccgac
      181 acggcatgaa ggcccgtttc ggcgccgtcg gcagacacgg ctccatcgcc ctcatcgagc
      241 gcctctggct cggcccgaag ggcctcctcc atctccggct cgatcgctgc ctcctgcggg
      301 acgacctcga gagcaggatc ggcctcgggc tcttctacga ctccgtcctc aagccccacc
      361 aaggcctcgg cggcgcgacg cccgccgagg tctacttcca gagtggaccg gcccgggacc
      421 acgcccttcc gccaccacgc gaaggcgacc cgatccctcc ccccgccttc gccgtccgct
      481 aactcgacgc cgcccgccgc ctgcccgtcc tcttccggaa ggccgcgtag ggccgcgacg
      541 ccggatcgcc gacgatccgg cccttctccg acccttccgt cctcgtccag cgcgctccgc
      601 ggtgacggcg acgcatcccc gcgtcgtcgc ggaccctctg ccgctgcgtc gtgattccgg
      661 tagcgaccgg gccgctcaag cccgtcccgg aggccattgg actcggacag gcgggccgcg
      721 aaccgcgctc gcgtcagggc cgccgcgcgt cgagggccgc gcgaaagcgt cgcttcagga
      781 gaaggccgtc tcgcggcgga agcgcccgag gcatcgtctc ggcgcgaacg acgacctgtg
      841 ccaggccgca cccggcccgc tcccggatcc gctgcgcgag gcgcgggagc tccgccgcgc
      901 tcgagaccgt ctccacccac gcgagaccgc aggcgaccgc gacacccgag aggctcgttc
      961 cgaggctcgt gtgagtctcc tgcatcccgg tctcaccgta gcggccgttg tccagcacca
     1021 ccaccgtgag gttcggcggc ctccgcgctc cgatcgtcgc gagcgccccg agccccatga
     1081 gctgctcgcc gtcgccggtg agaacgagaa cgctccgctc cggctgcgcg agcgcgagac
     1141 cgagccccat cgcggccgcg ccccccatcc cgccccagag gtagaagctc gtgtcgcggt
     1201 ccccggccgc gaagaggtcg taggcgggcg cgccgagacc ggcgatcgcc aggacgtcgg
     1261 ggcaggcctc gatcaggtcc gcgacgaacc tccgccggtc gatctcgcgc gacggcgcgg
     1321 tcacttgccg tctccccagt ccttccggcc gacgagtcgt tgcgcgagga ggacggcgac
     1381 cgcctcctcc cgctcgaacg cggcaacgag agcctcgcgg acgactcccg ccaccgctcc
     1441 cggctcctcg gcacgcagga ccgtcacgcc catcgcctcg aggacggtcc tcgtcgccct
     1501 ccccatcggc tcctgccact cgttccgctc ttcccactcg ccgcgcatcg tgacgagggc
     1561 gaggaacggg aagcggcacg tcgagacgac cgagagcatg ttcacgcagt ttccgacgcc
     1621 gctcgactgc atgaggagaa cggcccggtc gccgccgagc gcggcgccgc aggcgagagc
     1681 gacgccctcc tcctcggtcg tgagcgggat gccgagcagt cccgggtcgg cgaggacgcg
     1741 atcgatcacg tgggcgtgcc cggcgtcggg cacccaggcg acctggcgga ccccggctcc
     1801 cttcagggcc gagaagacct cctcctgcca gggcgccggg accggatcgc tcacgctggt
     1861 cactccgccc ggagattctc caccgggtcg agcgcggccg cccggcctgc cggcgccacg
     1921 cccgcgacga ggccgacgac gagagccgtc gccagcgccg cgccggcgta ctcgggtgga
     1981 gtcgcgaacg gcaccgccgg gagggcgagc cagacgaggg acccgagcgt cacggcgagg
     2041 aggacgcccg cgagaccgcc cgctccggag aggatcgccg cctccgcgag gaagacgttc
     2101 cgcacgtcgc cgcgcgtcgc cccgagagcc ttcaggaggc cgatctcggc cgtccgctcc
     2161 ccgaccgcga tccacatcat cgtcaggatc ccgatcgccc cgacgaggag ggagatcgcg
     2221 ccgatggccc cgacgacgac gctcacgatc gcgaagacgc gcccgaacga ctcgagcatc
     2281 tccccctgcg tcatgacggt gaagtcctcc tcgccgccgt gccgcgcctt caggacccgc
     2341 ttcaccgccg cgacgaccgt ctccggcggg aggccggggg tgaacgtcgc gtcgatctcc
     2401 atcacctgcg cctggtcgaa gagctgctgc gcggaggcga cggggacgta gaccgcgtcg
     2461 tcgagcgtga agccgacgaa ctgccccacg gtcgccatga cgccgatgac gacgaagcgg
     2521 tggccgccga cacggatccg ccgcccgatg gcgttctccg cgccgaagag ctcccgcttc
     2581 agcttcggcc cgaggacggc gacgggagtc gcccggtcga gctccgtctc gggaaggaag
     2641 cggccgaggc gcacctggaa ccgccagacc tcaggcccgt ccgaggtcgc gccgtagacg
     2701 aacgtgtccc gcgcgcgctc cccggaagag acgcgcgccg tcccgaagac gaccgggacg
     2761 accttctcga tcccggggac ggcgcggagc gcctcggcgt cgtcgagcgt cagcggccgg
     2821 gtcgtcccgc cgacggcccc ggcgatcccg tgcgtctcga tcttcccgga cgtgacggcg
     2881 aggaggttcg tcccgaactg cgagaactcc gagaggatcg cctgccgcgt cccctcgccg
     2941 agcgacgtga ggaggacgac cgaggcgatg ccgatgacga ttccgaggac cgtgagcgcc
     3001 gagcggaggc ggtgggccgt cacggagccg agcgcgaggc ggaggacgtc ggcgaacgtc
     3061 acggcctcac ctccgcgcga gcgcgacgac cgggtcgagc cgagccgccc gccgcgccgg
     3121 cacaacgccg aagacgagcc cgacggagac cgagacgccg agggccgcgg caacggccca
     3181 ggcgggcggg gcgagaggga agtcgggata gagcgcgcga aacgcgagcg tcccgagtcc
     3241 ccccgcgagg aggccgagga cgccgccggc cgacgagagg atcgccgcct cgacgaggaa
     3301 gattcggagg acctgcccgc cgctcgcccc gagcgccttc aggagcccga cctcgcgcgt
     3361 ccgctcggag acggagacga gcatgacgtt catgatgccg acgcccgcga ccgtgagcga
     3421 gaccgccgcg atgccggcga gcgccgcggt caggatgccg agcaccttcc cgaacgtctt
     3481 catgaccgcg tcctgcgtca ggacggtgac gtcctcctcg ccgtcgtgac ggtccttcag
     3541 gacggcgagg atccccgcct tcgccgcctc caggtcctcc gaggtccgcg cctcgcagag
     3601 gacgcggaag accgaccgcc ggtcgaacat ccggaggtgg tgcgcgatcg ggacgaggac
     3661 gaactcgtcg aggtcgaggc cgacggagac gccgcgcggg acgaggacgc ccgtcacgcg
     3721 gaacttctcg tctccgaggc ggaggagctc gccgaccggc gaccgccccg gaaagagctc
     3781 ggacgccacc gtcgccccga cgacgcagac gcgcggcgcc cggtccgggt tgccgggagg
     3841 aatgaagacc ccttcccgga gcgcgaggcg ccgcacgtcc ttccagtccg acgtcacccc
     3901 ggcgacggtc aggtcgcggc tccgctcgcc gaaacgggcc gtgagccctc cgacggagag
     3961 cggcgccacc gacacgagga gcgagacgcg ccgccggagc gcctcgacgt cctcgagcgt
     4021 caggtcgtgc ggcacgccgc ccaccgggac gacgccggtc gtctccgcct tcccggggag
     4081 gacgatgacg aggttcgagc cgaggagggc gaactccccc gtcacgtagc gccgcgcccc
     4141 ctctccgagg ctcgtcagga ggatgacggc cgccacgccg atcgcgacgc cgaggagcga
     4201 gagcgaggtc cggagcctgt ggccccggaa cgccccgccg gcgaatgcga ggaggtcgcc
     4261 gcctctcacg ccgcctcgtc tcccacgacg ctcccgtcgg cgaggcgcac ccttcgcctc
     4321 gcgcgcccgc cgatgccggc gtcgtgtgtc acgacgagga gcgtgatccc cgaggagtgg
     4381 agccgctcga ggagcgcgac gatctcggcc cccgaggcgc tgtcgaggtt gccggtcggc
     4441 tcgtcggcga ggacgaggcc gggccgcgtg acgaccgccc gcgcgaggca gacgcgctgc
     4501 ttctcgccgc ccgagagctg gtcggggcgg tggtcgagcc ggggcgagag gccgacggag
     4561 gcgagcgcct cggcggcgcg agccgtccgc tccttcggcg cgacccccgc gaggaggagc
     4621 ggaagcgcca cgttctcgag cgcggtcatc cgcggaacga ggtggaacga ctggaagacg
     4681 aacccgatcc gctcgcggcg caggcgcgag agctcctcgt cggggaggct cgccacctcg
     4741 cgcccctcga agcggtacga gccggcggtc ggccggtcga gcccgccgag gagggagagg
     4801 agcgtcgact tccccgagcc cgacggcccc atgacggaga cgtactcccc ggcgccgatc
     4861 gagagcgtca cgtcgcgcag ggcgtggacg gggcgaccgc ccacctccca ggtccgcgac
     4921 acgccggtca gctcgatcac cgcgcctcct cggcgcgcgc cctcgccccc tccttcacct
     4981 ccggccggtc gagcgacgtg acgacgaggt cgccggcgga aaggccggag acgacctcgg
     5041 cccactccgc ccccttcagc cccgtctcga ccttcgcctc ggcgagcctc cctttcgaga
     5101 ggacgagggc ccggcctccc ggcaggatcg cgtacgaagg cacccggaga acgtcctcct
     5161 ttcgcgcgag gacgacgacg gcgtcggccg acgctcccgg gaggagcgtc cgcgcgaagg
     5221 cggcgtcgtc gagcgccacc tccacctcga acgtccggtt ctgctccctc tggtcgagga
     5281 cgtagtcggc gatgcgcctc acccgaccgg cgaacgtccg accgggatac gcgtcgatcg
     5341 tcacgcggac ctcggcgtcc ttcttgacac gccccaggtc cacctcgtcg agcggggcgc
     5401 tcacgtagac gtcgtccggg tcgaagagct cgacgaccgg cggaatcgga aggccgggcg
     5461 gcgatggggt tacccactcc ccctcctccg tcgacatctc cgccacgacg ccgtcgaacg
     5521 gcgccttgag gaccgtcttc tcgagggcga cgcgcgccac gtcgagggag gcgcgcgcct
     5581 gccccacgcg ggcccgcgcc gcgtcgcatg ccgccgccgt catcccggcc tcggtctcgg
     5641 ccttctcgag gaggccgacg gagaggacct cgtccttcgc gaggctccgg ctccgcgcga
     5701 ggtcgcggcg cgcctgctcc gcagcccggc aggcctcgac ctcggccgcc ccggcgacga
     5761 cgagcgactt ctccgcgagc gcgacctgcg ccctcacgtc ctccgccgcg atccggacga
     5821 ggaggtcccc cttcctcacg cggtctccct cgcggacggc gagccgttcg acgcggccac
     5881 ccacctcggg gctgagggtc gcgcgccgcc gggacttcac cgtcccggcg cggctccccg
     5941 ccaccgtctc ctcgacggcg cccttcgcga cggcgacgac ggtcacgggg agggggtccg
     6001 ggcggaagag cgtcacccgg aggagaagca cggcggcgac gatcccgagg aggacgccgc
     6061 ccctgcgaag ccacgcctgt ttcatcagac ggacatcgta ggagccgggc gaggcgttgg
     6121 gaagcgcgcc gacgggtgcc gggcgacgtc cgtctagaat cccgcctcca tgaatcgggc
     6181 tcctcgtctc gccgtagcgt ccgccttcct ggccctcgcc gcctgctcgc ggagcgcccc
     6241 ggagccgcgg gtcggacggg aggagcggaa ggcgctcgcg gcgcaggcgg gctccacgct
     6301 cggagtcctt ccggagagga tgccgggagc ggaggccgat tccccggagc tcgtcgccct
     6361 cggccggtcc ctctggttcg agaggagact ctcggtcacc gggacgcagt cctgcaacga
     6421 ctgtcaccgc ctcgacggcc agaatccgac cggcgccgac gtcgcgccga cctcgaacgg
     6481 cgcgcgggga acgaaggggc cgcgcaactc cccgagcgtg aagaacgccg gctaccacgt
     6541 cgcgcagttc tgggacgggc gcgcccggac gctcgaggag caggccgagg ggccgatcct
     6601 gaacccggtg gagatggcga tgcccgacgc ggccgccgtg gaggcggtcc tccgcggcgc
     6661 cgccgagtac ggcgcgccgt tcgccacggc ctttcccggc gtgccgcagc cgatcacgct
     6721 cccgaacacg gcccgcgccc tcgccgcctt cgagaggacc ctgaagacgc gcgaccgcct
     6781 cgacgacttc ctccgggggg acctcgaagc cctctcgcac cgcgaagccg cgggcctgaa
     6841 gctcttcctg acgacgggct gcacgacgtg ccacaactcg ccgaccatcg gggcgaacca
     6901 gttccaggtc cttggcctcg tgaaggagtg ggagacgaag gacgagggtc gcttcgccgt
     6961 gacgaagaac gaggaggacc ggcggaagtt caaggtcccc tcgctccgga acgccgtcgc
     7021 cacgggaccc tacttccacg acggctccgt cgaccgcctc gaggtcgccg tgaagaagat
     7081 ggcctggcac cagctcggca aggagctgac ggacgacgag atcgcctcga tcgtcgcctt
     7141 cctcggggcg ctcgccgacc ggagcggccc catcctcccg cccgctccgt cgacgaagga
     7201 ggccgaccga tgaatcacga atcgctcgtg aaggacgccg ccgcccgctc cggcctgccg
     7261 gaggcgacgg tcgagaaggt cgtggcggcc ctggccgacg ccgtccacca cgcccacgcc
     7321 acgcccgacg agctcctcca cgcgctgctc ggcgccgacg agccgctcca cgcccacccc
     7381 gtcgacccgc gcgacgcggt cgtcgtcgcc gacctcctcg agcgggcgag ggcgcatccc
     7441 ctcggcctcg actacctgaa ggggggccac ctcgggagcg tcgccgtcac gttcgagacc
     7501 cacgcgttta ccgtgctcgc cgcccgcgag cggctgaggt gaacatgaag agctggaacc
     7561 tcccgaagcg cgtcgacctc gccgacatca ccgtccgcga cggcttccag cacgaggaga
     7621 ggtggatccc gaccgaggcg aagctctggg tcctcgagga gctggtcctc gccggctacc
     7681 ggcgcctcga ggtgacgaac ctcgggaacc ccaaggggat gccccagttc cgcgacgcgg
     7741 acgcgctcct gaaggggatc cgcggcagcg cccgcgtctc gaagcagatc ggcgaggtgg
     7801 agctgacggc ggtgacgatc cgggagaagg ccgtcgaccg cgcgatcgcc gcgaagtccg
     7861 aagggtgggg gcccgaccgg atcctcatga tggtctccac ctcggcctcg cacatgaaga
     7921 agaactccgg cctcgaccac gccgggtact gggccgaggc ggagcgctgc atccggaagg
     7981 cgcacgacgc cggcatccgc gtgaacggga ccgtcagcac gatctggggc tgcccgatcg
     8041 aggggccgac gaagctcgag gacgccgtcg cgttcacgaa gcggttcctc gacatcggcg
     8101 cggacgacat cgagcacgcc gaccacgacg gctccgctcc cccggaccgg gtctacgcgt
     8161 actactccat tgtcctcgac gccattccgg acgtgacgaa gcacctcgcc cacttccacg
     8221 tcacccgcgg ctggggcctc gcgaacgtcc tcgcggcgct ccaggcgggc atcgttcgct
     8281 tcgagagcac cctcggcggg atcggcggcc agccggcaaa cttcttcgag ggcgcccccg
     8341 tctccggcac cggccgctac tactaccagg acccgaacaa cgtcggcctc gtctgcaccg
     8401 aggacctcgt cgtcatgctc gacgagatgg ggctcgagac cggcctcgac gtcgaccgcg
     8461 tcctcgcgac gggccggatg gtcgagcgga tcgtcggccg gcgcctccgg agcgaaacgg
     8521 tcaagtcggg ccggatcccg aaaaggccga ccggcttctg acgactccgc tcccggggtc
     8581 ctgagcgagc ggccgaccca tccggccgct cctccgccgc tggcgtgatc gctccccggc
     8641 tccggacaga gccgggcgct gtcgcacgcc tcctgctcgc tcgacgatca gccctccaat
     8701 caggaccgac acggggcctg ttcgcactcg ccgtcgtgtg gtaccttcca cgtttttttt
     8761 gagatcgtcg ctcaacagcg acccaccgcg aggagaggga tttgcactgt acagggaggt
     8821 cccggcacca cctccgcccg tccgcgacag ctccggggaa tcggcccgaa tccgtaacgg
     8881 ccccgggagg ggtcgaaatt ccccgcccgt ctccgagaaa ggcataagcg cagatggccc
     8941 agctctttcc gaagagcgcg aactctctcg cgcccgcctc cctcgccgcg ctcgtcctcg
     9001 tggcgggagg cgccgtcggc tcgctcctgg cgctcgacta cgccggcttc aaccagcggc
     9061 gcggcgagac ggtcgagcag aaggtcccgt tcagccacga gcaccacgtc gccggactcg
     9121 gcatcgactg ccggtactgt cacacctccg tcgagacgtc ggccgtggcg ggcgtccccc
     9181 cgacgtccac ctgctacaac tgccacaaga ccgtctggaa cgaggcgccg atgctcgagc
     9241 ccgtccgctc gagcgtgaag acgggccagc cgctcgagtg gacgagggtc cacgacctcc
     9301 ccgacttcgc ctacttcaac cactcgatcc acgtcgccaa ggggatcggc tgcgccacgt
     9361 gtcacgggcc ggtccacgag atgcgcctca tgcgctcggc ccagccgctc cagatgcgct
     9421 ggtgcctcga ctgtcaccgg aaccccgaga agttcatccg gccgaaggaa gagatctaca
     9481 acatgacctg gaaggcgaag gaccaggaga cgctcgggcg cgagctcgtg aaggcctacg
     9541 gcgtccgccc gcccgccgag atcacctcct gcggaacgtg ccaccgatga gccggggcga
     9601 ggagcgagac ctgatgaccc cttcgagcct ccaccccggg cccgcggacg acacgcccgc
     9661 ccgcttcccg atcgacctcg ccgccatccg cgagcgcctc gacaggcgcc aggggccggc
     9721 cttctggcgc ggcctcgagg agctcgccga cgacgcgggc tttcgcgaat tcctggcgaa
     9781 ggagtacccc cgtcacgcct ccgactggga cgagagcctc gaccgcggct tctcccgccg
     9841 gcgcttcctc gagctcggca tggcctcgat ggccctcgca gggatgacgg cctgcacgcg
     9901 gcagccggtc gagaagatcg tcccgtacgt gaagcagccc gaggagatcg tcccgggcgt
     9961 gccgctgttc ttcgcgacgg ccgcgacgat cggcggcttc gcccagccgc tcctcgccga
    10021 gagccacgag gggcgcccca cgaaggtcga ggggaacccc gagcaccccg cgagcctcgg
    10081 cggcgccacc gccctggcgc aggcgtccgt cctcggcctc tacgatcccg accggctgaa
    10141 ggtgccgacg aagctcggcc tcctcgacac ctggccgcgc ctcgtctccg agctccaggc
    10201 cgcggcgaag gcccagaagc aggtggaggg ggcgggactc cgcatcctga cgcagaccgt
    10261 cacctccccc actctcggcg cgcagctcgc ggcgctcctc gccgcctacc cgaaggcgaa
    10321 gtggcaccag tgggagccgg ccggccgcga cgccgtgcgg gccggcgccc gactcgcctt
    10381 cggacgcgcc gtcgagaccc gctacgacct cggcgccgcc gacgtcgtcg tcgccctcga
    10441 ctccgacctc ctcgcctccg gccccgactc cgtccgcaac gcgaaggcct tctccgcgcg
    10501 ccgccgcctc acgggcggaa agacggagac ctcgcgcttc tacgccgtcg aggtctcccc
    10561 gaccccgacc ggcacgctcg ccgaccaccg cctcccgctc cgggccagcc ttctccccgc
    10621 cttcgcgcag gcgctcggcg ccgccctcgg cctcccgggc ttcgcggccc ccgccgggct
    10681 cgacgagagc gcggcgaggt tcgccgcgac cgcggcgaac gatctgcgcg cggcggccgg
    10741 acgctccgtc gtcgtggcgg gcgacttcgc ccctcccgag gtgcacgccg cggcgcacgc
    10801 gatcaacgag gcgctcggga gcctcgggaa gaccgtcttc cttaccgagc cggtcgaggc
    10861 cgccccgatc gaccagctcg cctcgctccg cgagctcgcg gaagacgtga aggcgggctc
    10921 cgtcgagctc cttctgatcc tgggcgggaa cccggtctac gacgcgccct ccgacctcgc
    10981 gttcggcgag gcgctcctga aggcgggcct gacggtcgcc ctcgccagcc accccgacga
    11041 gacgacccgc tactgccact gggccgtccc gatggcccac gacctcgagg cgtggagtga
    11101 cgcccgctcc ctcgacggga ccgcggcgct cgtccagccg ctgatcgagc cgctctacga
    11161 ggggaagtcg gcgcacgagc tcctctccgt cctcctcgac gaggtccccc ggaagggcta
    11221 cgacgtcgtc cgggaggcct ggaaggcggc gcgccccgcc gaggccgact tcgaggcgtt
    11281 ctggcggaag gcgctccacg acggcgtcgt ccccgggagc gccgcccccg ccgtcgcggc
    11341 gccggtccgc ctcgccgagg tggccgccgc gctcgccgcc gcctccgccg tgaagcccgc
    11401 gggactcgag gcggtgttcc gccccgaccc ctcgctcctc gacggccgct tcgcgaacaa
    11461 cggctggctc caggagctcc cgaagccgct cagcaagcta acctgggaca acgccgcggt
    11521 cgtctcgccc gccaccgccc aggagctcgg cgtcacgaac gaggacgtcg tgaaggtcga
    11581 ggcgggcggg cgctccgccg agctccccgt cctgatccag ccgggccagg cggcgggcgt
    11641 cgtcacgctt cacctcggct tcggccggaa gcacgcgggc cgcgtcggca acggcgtcgg
    11701 cgctgacgtg aacccgctcc ggaccacggc cgccccctgg ctcgtcccgg cggtcaccgt
    11761 gacgaagacc ggcgggcgcc atcctctcgc ccacacgcag cagcacttcc ggatggaggg
    11821 gcgcgacatc gtccggacga tgacgctccc cgagtaccgc aaggacccgg cgaaggcgaa
    11881 ggggcacggc ctcccgccga aggaggcctc cctcttccag ccgggctggg agtacaagga
    11941 gaacgcctgg ggcctctccg tcgacctcgg ctcctgcttc ggctgcaacg cctgcgtcgt
    12001 cgcctgccag tcggagaaca acgtccccgt cgtcggcaag gagcaagtct cgaagaaccg
    12061 cgagatgcac tggatccgcg tcgaccagta ctacgagggg agcgacctcg agaacccggc
    12121 cgtccaccac cagcccctca tgtgccagca ctgcgagatg gcgccgtgcg aggtcgtctg
    12181 ccccgtcgcc gcgacggtcc acggagcgga ggggctgaac gagatggcct acaaccgctg
    12241 cgtcggcacg cgctactgct cgaacaactg cccctacaag gtccgccgct tcaacttcct
    12301 cgagtacaac ggccagaggg aggaggagcc ggtcctgaag atgctcgcga acccggacgt
    12361 caccgtccgc tcgcggggcg tcatggaaaa gtgcacctac tgcatccagc ggatcaatcg
    12421 cgcgcgcatc gccgccgagc gcgaggggcg tgcgatccgc gacggcgaga tcaagaccgc
    12481 ctgccagcag acctgcccgg ccgacgcgat cgtcttcggg aacatcaacg acccggcggc
    12541 gggcgtctcg aagctccgga gcgagccgcg gacctactgg ctcctcgacg agctgaacac
    12601 ccgtccgcgg acgacctacc tcgcccgggt cacgaacccg agccccgacg tggagggcgc
    12661 ctgacatgtc gactgcctac gacgacgtcc gcggcgccga cccgatcgcg cagcctcccg
    12721 tcctcgcccc gggccacacg ccggcctccg tcaccgagcg gatcagcgag gccgtcacca
    12781 cgaagctgac gaagacgccg aagtggtggc tcggcgggtt cgccgtcggc ttcgccctcc
    12841 tgaacgtctt cctcttcgcc gtcacgaacc tcttcatgac cggcatcggc gtctggggga
    12901 acaacatccc ggtcgggtgg ggctgggaca tcatctgctt cgtctggtgg atcggcatcg
    12961 ggcacgcggg gacgctcatc tccgcgatcc tcctcctcct ccggcagagc tggcgcaact
    13021 cgatcaaccg cttcgccgag gcgatgacgg tcttcgccgt cgcctgcgcc ggcatgtacc
    13081 cgatcctcca cctcggccgc ccctggctcc cccactggct cctcccgctc ccggggacgc
    13141 tcggcatgtg gccgaacttc cggagccccc tcatctggga cgtcttcgcc gtctcgacct
    13201 acgcgacgat ctcgatcgtc ttctggtacg tcgggatgct ccccgacctc gcgacgctcc
    13261 gcgaccacgc gaaggcgaag ttcgcccgct tcgcctacgg catggcggcg ctcggctggc
    13321 gcaactccgc gcggcactgg gcgaactacg aggtcgcctc gctcctcctc gcgggcctct
    13381 cgacgccgct cgtcctctcg gtccactcca tcgtctcgtt cgacttctcg gtcggcatcg
    13441 ttcccgggtg gcacacgacg gtcttcccgc cctacttcgt cgccggggcc gtcttcgccg
    13501 gcttcgcgat ggtcctcacc ctcgtcctgc cgctccgccc gctcttcaag ctccacgact
    13561 tcatcaccga gcggcacctg cagaacatgg cgaaggtgat gctgacgacc ggcctcatcg
    13621 tcgcctacgg ctacgtcctc gagatctggg cggccttcta cagcgggaac gagttcgaga
    13681 tcttcatggt cctgaaccgc ttcacggggc cgtacgccac gcagtactgg ctcctcatcc
    13741 tcctcaacat cgcgatcccg cagctcctct ggctcaaggg ggtccgctcg aacgtctggg
    13801 cgctctgggc gatctcgatg tgcatcaacg tcgggatgtg gctcgagcgg ttcgtcatca
    13861 tcgtcgtcag cctccaccgc gactacgtcc cctcgaactg ggcgatgtac tccgggacga
    13921 agttcgacgt gatgacgttc gtcgggacga tgggcctctt cctgacgctc ctcctcctct
    13981 tcatccgctt cctccccgcg ctgaacatgt tcgagatgcg cgtcctgacc cccgaggcca
    14041 cgaaggccca cggcgaggag aagtagcgtc atgcacggca cgcaccccga gccgtccctc
    14101 cacggcgtcc tggccgagtt ccgcacgccc gacgagctcc tcgcggcgat cgaggcggcg
    14161 aagtcggcgg gctacacgaa gctcgacgcc tataccccct acccgatcga gaaggtcctc
    14221 gacgccctcg acctccatca ctcgaaggtc ccgctcctga cgctcggggc ggcgatcttc
    14281 ggcgcggtcg cggtcttctc cttcgcctac tgggcgagcg cgatcgacta cccgctcaac
    14341 atcggcgggc ggcccctcaa ctcctggccc gccttcattc ccgcgacctt cgaaggcgcg
    14401 gtcttccacg gcgggctcgc ggccgcgctc ggcatgctcg tcctgaacgg cctcccgcgg
    14461 ccgcaccacc cggttttcga ggtgaagagg ttcgtcgagc acgcctcgaa ggacggctac
    14521 ttcctcctcg tggaggccga cgacccgagg ttcgacgtct cggccgtccg gcggctgctg
    14581 gagggcctcg cccccgtgga ggtcaacgac cttgaaaact gaccttccgc ccggccgccg
    14641 gctcggcgtg gcggcgctcg ccgtcgcggc cctcctcggc gcgggctgcc gccaggggat
    14701 gtacgaccag ccgaaggcga agccgctcgg ccggagcgac ttcttccgcg acggcctctc
    14761 ctcccgcgtc ccgcccgcgg ggaccgtcgc ccgcggctgg ctccgcgagg accgcgtcct
    14821 ccacaccggg atcggccccg acgggaagtt cgtctccgag cttcccgggg ccgttccgtt
    14881 cgacaaggcg ctcctgctgc gcggacgcca gcgctacgac gtcttctgct ccccctgtca
    14941 cggccggcag gggaacggcg agggaatgat cgtccagcgg ggcttcaagc agcccgtctc
    15001 gttccacgtc gaccgcctcc gcgcccagcc gctcggctac tacttcgacg tgatgacgaa
    15061 cggcttcaac gagatgtcga gctacgccag ccaggtcccc gtgaaggacc gctgggccat
    15121 cgcggcctac gtccgcgccc tgcagctctc ccgcaacgcc cccgcctccg agctctccgc
    15181 cgcggacctc gaggcgctcg agaaggctgc cgcctccgcg gccgcgccgg aggtccaccc
    15241 gtgaaccagg gctcgaaccc cctccccgag ggcgtcgaga agctcggccg gatcggcctc
    15301 gtcgccggcc tcgtcggcct cgccctcctc ggagccggat acgccaccga ccgagatcag
    15361 ctcttccgct cctacctcac cggctacatg tggatgctcg gcctcgccgc cggcgccctc
    15421 ggcgtgatgc tccttcacca cgtcacgcgc ggcgcctggg gcgtggtcat ccgccggatc
    15481 ctcgaagcgg cggccagccc gctcacgctc gggttcgccg cggtggcgtt cctgccgatc
    15541 gccctcggct cgcactccct ctacgagtgg acccacaccg acgtcgtcgc cggggacgcg
    15601 atccttaccc acaagaagcc gtggctgaac gagcccgcct tcctcgggcg ctccgcggcc
    15661 tacctcgtcc tctggatcgt cctcgccgcg gtcttcgcgc gcctctcggc ccggcaggac
    15721 gagacgggcg accgccggat cgccctctcg atgcagcgct ggggcgcgtt cggcgtcgtc
    15781 gcgttcattc tctcgatgac cttcgcggcg ttcgactgga tgatgtcgct gacgccgcac
    15841 tggttctcca caatctacgg cctctacgtg atcatcgggc aggccgtcgc ggcgatggcg
    15901 ctcgtcgccc tcgcggggct cctcctcggc gagggacccg cgcccgccct ccctctccag
    15961 gcgcggcacc tccacgacca cggcaagctc ctcttcgcgt tcgtctgcgt ctgggcctac
    16021 ttcggctact cgcagttcat catcatctgg tcgggcaacc tccccgagga gacctccttc
    16081 tacgcttcgc gcctgaacgg ggcctggaag gcgacaacgg tcgtcctcgt cctcctccac
    16141 tacgcgctgc cgttcgccct cctcctctcg aggaaccgca agcggagcgc gaaggcgctc
    16201 gcgccggtcg ccgtcctcct cctcttcgcc cggtggctcg acctccattg gctcgtcgcg
    16261 cccgcgttct cggcgaacgc cttcacgctc cactggctcg acctggccgc gccgctcgcc
    16321 ctcggcggga tctggctccg cctcttcacg ctcgggctga aggcccggcc gctcctcccc
    16381 gcgaacgagc cgttcctgaa ggaggcgctc gcccatgagt gaccaccccc gcccgcagca
    16441 cgcgccggcg gagttcgatt ccgagatcca cgtcaaggcg atcttcggcg tcctcgcggg
    16501 cctcgcgctc ctcatcgccc tcgcgttcgg cgggatgatc gccttcagcg ggtacctgaa
    16561 gtcccgctcg atcgcgcggg accccgagcc gctacccctc gccgaggcga acgctccgcg
    16621 gccacgcccc cgcgccgcgc tccaggccga cccgaccgcc gacatgacga agttcgcgcg
    16681 cgaggaggag gcggccgtct cctcgtacgc ctgggtcgac cgggccgcag gcgtcgcgca
    16741 gattcccgtc gagcgcgcgc tcgagatcgt cgccgagcgc ggactccccg tcccgccgcc
    16801 gctccccgag gccggccccg ctccggccgc aggaggctcg aaatgagccg caccgttcgt
    16861 ctggccggac tcggcctcct cgccgtcctc gttgggagcc ccgccctcgc gcagccgatg
    16921 atgccctccc cgacgcgcga ggtcggctgg gagcagaaca tgggagcgca gctcccgctc
    16981 gacgcgaagt tcaccgacga gaacgggaag gccgtccgac tcggcgacta cttcggaaag
    17041 cgccccgtcc tcctctcgct cgcctactac gagtgcccga tgctttgcgg catcgcgctc
    17101 gaggggctcg cgcggagtct gaagggcttc accctgacgc cgggcgccga cttcgaggtc
    17161 gtcacgctca gcttcagccc gaccgagggg cccgagctcg cgaaggccaa gaagtcgaac
    17221 ctcctcgacc tctacggccg gaaggccgaa gggcaggcgg gctggcactt cctgacggga
    17281 agcgacgccg agatccgccg cgtgacggaa gcggtcggct tccgctatcg ctgggacgag
    17341 ctccagaagc agtacgccca cgcgaccggg atcgtcgtcg tcacccccga gggggtcatc
    17401 tcgcggtact tcttcggcgt cgagtacgcg ccgaaggagc tccggctcgg cctctccgag
    17461 gcctccgagg ggaaggtggg cggcgtcgcg gcgcagctcc tcctcctctg cttcgagtac
    17521 aaccccgccc tcggcaagta caccgcggag acgatgacgg ccctccggat cgcgggcggc
    17581 ttcctcgtcg tcgccttcgg cgtcttcctc gcggtctcgc tccgccgcga gcgccggagc
    17641 cgcgcccatc tcggagccaa cgcctgaaca tgctgccgaa cgcgcccgtc ttcccgccgg
    17701 tctcctcgac cctcgcctcg aaggtcgaca ccctcttctt cttcacgctc ggcgtctcgg
    17761 tcctcttctc cgtcgcgatc gtcgcgacga tcgcggcctt catcgtccgc tatcgccgcc
    17821 gccgcgccga cgagacgggc caggagatca agggaaacac cctcctcctc gagctcgcct
    17881 ggtcgatcgt cccgctcggg ctcgtgatgg tcagcttctt ctggggcgcc tcgatcttct
    17941 tcgagtcggc ccggcctccc gccgacgcct ggtccttcca cgtctacggc aagcagtgga
    18001 tgtggaaggt cgagcatccc gaggggaagc gcgagatcaa cgagttccac gtcccggtga
    18061 accaggcggt gaagctgacg atgacctccg aggacgtcat ccacaacttc tccgtgccgg
    18121 cgttccgcct caagatggac gtcctgcccg gccgctacac gaccgcctgg ttccgggcga
    18181 cgaagaccgg cacctaccac atcttctgcg accagtactg cggcgtcgac cactccaaga
    18241 tggtcgggaa gatccacgtg atggagccgc aggactacaa gcgctggctc gtctccggcg
    18301 cctcggccgg cggcgccgtc gcgagcggcg aggagctctt cgtcgcgaag gcgtgcaaca
    18361 cctgccaccg ccccgacagc tccgcccgcg cgcccgtcct cgacggcctc ttcggaaaga
    18421 aggtcgcgct cgtggacggg aggaccgtca ccgtcgacga gagctacgtg agggagtcga
    18481 tcctctcccc cgcgacgaag gtcgtccagg ggtaccagcc gatcatgccg acgttccagg
    18541 ggcagatctc cgaggaggag gtcgtccagc tcatcaagta catcaaggac ctcccgcccg
    18601 ccggagcccc cggcgcgccg gccgccgctc ccgccggagg aaagccgtga ccgctcccgc
    18661 gaccgccgcc ccgaacgcgc tgccgagcta cctcgaggcc gcgcacggcc cgaagtcgtg
    18721 gctcctgacg agggaccaca agaggatcgg gatcctctac ctcgtcgcca tctccgtctt
    18781 cttcgtcctc gggggcctga tggccgtgct catccgggcc gagctcgcga cgccgcaggg
    18841 ggacatgttc tcctccgacg tctacaaccg gctcttcacg atgcacggca tcctgatgat
    18901 cttcttcttc ctgatcccgt cgatcccggc ggtgctcggg aacttcctcc tgccggtcat
    18961 gatcggggcg aaggacctcg ccttcccgaa gctcaacctg acgagctggt acctctacat
    19021 gatcggcggg ctgatgacgt tcggctcgct cctgacgggc ggcatcgaca ccggctggac
    19081 gttctacacg ccgatgtcga cgacgttcgc gcaggggcag gtcctcgtca cggcggtcgg
    19141 catcttcgtc gccggcttct cctcgatcct gacgggcctc aacttcatcg tcacgatcca
    19201 ccggatgcgg gccccgggga tgacctggtt ccgcctcccc ctcttcgtct gggcgcacta
    19261 cgcgacgagc atcatccaga tcctcgggac ccccgtcgtc gcgatcgcga tcctcctcgt
    19321 cggcgccgag cggctcttcc acatcggcgt cttcgacccg aagtacggcg gcgacccgct
    19381 cctcttccag cacctcttct ggttctactc gcaccccgcc gtctacatca tgatcctgcc
    19441 ggcgatgggc gtgatgagcg agctcgtcac cgccttctcg cgcaagcggg tcttcggcta
    19501 ccacttcgtc gccttctcga gcctcttcat cgccgtcatc ggcttcctcg tctggggcca
    19561 ccacatgtac gtctccgggc agtccgtcgt ggccggggcg atcttctcgt tcctgacgat
    19621 gctcgtggcg atcccctcgg ccgtgaaggt cttcaactgg acggccactc tctacaaggg
    19681 ctcgatctcg ctccagacgc cgatgctcta cgcgctcggc ttcatcggcg tctttctgat
    19741 cggcggcctg acgggcgtca tgctcgggac gctcgggatc gacatccacg tccacgacac
    19801 ctacttcatc gtcgcgcact tccactacgt catggtcggc agcgcgatca tgggatacct
    19861 cggcggcctc cacttctggt ggccgaagat caccggccgg atgtacgacg agttctggtc
    19921 gaaggtcggc gcgctcctga tcttcgtcgg cttcaacacg accttcttcc cgcagttcat
    19981 cctcggctac ctcgggatgc cgcgccgcta ccacatgtac ccggcggagt tccagacgtg
    20041 gaacgtcctc tcttccgccg gcgcgacgat cctgggcgtc ggctacctca tcccggtgat
    20101 ctacctcctc tggtcgctga agttcggggc gaaggccccc gcgaacccgt ggggcgcgaa
    20161 ggggctcgag tggcagaccg cgtcgccgcc gccggtcgag aacttcgcga cgccgccggt
    20221 cgtgacggag gaggcttacg cgtacgagac ctccgtaccg gccaaggagg tgaccgttgg
    20281 catctgaggc ccgccccgcc cccctcgccc accacttcga cgactacgac cagcagcgcg
    20341 agtcggccac gctcgggatg tggctcttcc tcgcgcagga ggtcatgttc ttcggcgggc
    20401 tcttcctcgc ctacaccgtc taccgctgga agtaccccgc ctggttcgcc ctcggctcga
    20461 accagctcga cgtcccgctc ggcctcgcga acaccatcgt cctcatcggg agctccctca
    20521 cgatggtcct cgccgtccgc gccgcccagc tcgggaaggc gcggcagatc gccggctgga
    20581 tcggcgcgac gatggcgctc gggacggcct tcctcgtcgt caaggggttc gagtaccacc
    20641 acaagtgggt ccaccacctg atcccggggg ccggcttcca catcgagggg acgaccgacc
    20701 cggcggcgca gacgttcttc gtcctctact tctgcatgac ggggctccac gccctccaca
    20761 tgatcgtcgg cctcggcctc ctcgccttcc tcgtcccgaa ggcgctccgc ggcgacttct
    20821 cggccgagaa ccacaactgg gtcgagggcg tcggcctcta ctggcacttc gtcgacgtca
    20881 tctggatctt cctcttcccg ctcctctacc tcctggggcg gcaccacgcc tgaggccgac
    20941 ggagtagcca tgtcgaccgt ccacgtcact cccgtccgca cctacctcct cgtcttcttc
    21001 gggctcatgg cgctgacctt cctgaccgtg ggggtcgcgc acgtgaacat cgccgatcac
    21061 ctccccggcc aggtcaccga cgcgatcaac gacgccgtcg cgatggcgat cgccgtgacg
    21121 aaggcgacgc tcgtcgtcct cttcttcatg cacgtctggc actcggcgcg cctgaacaag
    21181 atcgtcgtct gggcgtcgct cttcttcatc ctgatcctct tcgccttctc gctcgccgac
    21241 tacttcagcc gcggctggct cggcgtaccg gggaagtagc tcccgcgggg aatgcccgaa
    21301 aaagagaggg gccccggcgt cgccggggcc tctcttcgtc gggtgaatcg agcgatcgcg
    21361 cggggctacg gtttcgcgtc ggccagggcg atcgccgccc cgaagaccgt gcgcgccccc
    21421 gattccatcg tcttgggcgt gacgaagtag accgtgtcct ccggcgtgtg gtagccgtgg
    21481 tggtcgcccc ccatcgcgaa gagcgagacg gcgtggatcc ccttctcgaa gaacggcgcg
    21541 tggtcggcgc gcccctcgcc gcggatcttt cccgatctca ggttgaaacc gggcgcgagg
    21601 cggtcgcgcg cccgttccat cgccgccttg atctcgggga agttctcccc gccggcgacg
    21661 tagaggccgt tgccggcgcc gagcatgtcc atgttcagca ccgcgacgca cgtctcgagc
    21721 cccttcgggg gatggctcgc gaaatgcgtc gcgccgagga ggccgagctc ctcggccgcg
    21781 aagaagacga acgcgatcgt ccgccggggg cggaccttca gcgtcgcggc cgcccgcgcg
    21841 acctcgagga ggctcgcgga gccggaggcg ttgtcgtcgg cccccgggtt gaggcggggc
    21901 cattcgccga tgtggtcgag gtgcgcgccg acgacgacgt attccccctt caccgacggg
    21961 tcggagccgg ggaggagggc gacgacgttg tgcgtcgtca cctcgcgcca cgggcgcccc
    22021 ttcacctgga agcggagccg gctcccggtc gcgaaggagg cgaggccccc cttcgagagg
    22081 acctttcgga gctcggcgac ggtcagcttc ttcccggcga ggagccggtc ggcgaacgcc
    22141 tcgctcacca tcgcctcggg gaggtcgcgg ctgatgccga cgctcgtcga ggtgaccggg
    22201 ctgtcgatca ggaggagccc cgccgctccc ttccgcgccg ccgccttcgt ccgggcgacc
    22261 gtcgagcggt ggtccttcca ctcgcgcccc tccggctcgc cccggaggac caggacgacc
    22321 ttccccctca cgtcgacccc ggcgtagtcg tcgcgccctt cggaggcggc gtcgaagccg
    22381 tagccgacga agacgacctc ggccgtgacg tctccggagc cgccgttcac catcggggag
    22441 aagtccttca ggacctccgc ctcgtacggc gtgccgtcct tctctccctc ggcagggagg
    22501 agcgtcaggc tcgcggcctc gacgcccgcg aggccgtgcg tgaaggcctg cctgtagtcg
    22561 ggaaggtccg cgggcgggcg caggcccgcc ttgcggagct cggcgacgac gtggtcggcc
    22621 gccttccggt agccctccgt cccgctgagg cgcccggcca tcccgggcgc ggcgaggacc
    22681 tcgaccgtcg tgaacgccga cgcagcggtg atccgcgagg cggcggaggc gacctcggcc
    22741 tccgtcggac ccgccgcccg cagcgcaccg ggaacgatta gaaacgcgcc ggcgaggagc
    22801 ggaaggagcg ctcgccgctc cctcgcgacc cgcccctcgg tcacttcttc acctcggagg
    22861 gaggggcggc gggcgcggcg gcggccgggg ccttcccctt cagcgcggtc aggacctccg
    22921 cctccttttc cgcggcgagg ccggcccgcg ccttcgcgcg cctctcctcc gggagcgagt
    22981 cccaccagtg gagggtggag gcgatcgtct ccgggagcgg gcggaacgtc agccccgcct
    23041 tcagggcgcg cgcgatcgag acgtcgccga tcccggcctc ggggccttcc gtcgagaccc
    23101 agatcgggaa gtcggcctcg ggcgtcagcc ccagctcctt cagcttcgcc tcgggcgccc
    23161 aggtgaacgt cgcgttcgac ttcgtctcct tccggcacgt ctcgaggaac gccttcatcg
    23221 tgagccgctc cttcgggccg gtcgcgttga agacgccgtt cgtcccggac tcgaccatcc
    23281 ggacgatcca gtcgcccagg tcgcggacgt cgatgaactg gagcgggtcg gccggctttc
    23341 cggcgacgag gacctcgccc cccttccgga cccggacggg ccagtaggtg aagcggtcgc
    23401 tcccgtcccg gtccccgacg atatagccgg gcctcacgtt gagggccttc cccggcatcg
    23461 ccttctcggc cgcctcctcg cagaggagct tcagcgcgcc gtagttcccg ccggtgatgt
    23521 ccttgatcgc gtcgacgtcc gtccccgccg cgaggcgcgc gacggccgcg gtctcgtcca
    23581 tcccggcctt catcggcgcg tcgtagaccg agatcgagga gatgaagacg tactggccga
    23641 cgttccccga aaggacctcc gcggcggagc ggacctgccg cgggaagtag ccggaggtgt
    23701 cgatcacggc gtcccacttc cggccggcga gcttcttcac gtcgtcggcg acgttccggt
    23761 caccgtggat ctcctccagc gggcgcccct tgaagaggcc ggggttcgac ttgccccggt
    23821 tgaagagggt cagctcccac cccttcgcca gggccgcctc cacgatggcg accccgagga
    23881 agcgggtccc cccgaggatc aggaccttct tcttcgcggg cgcggcctcg gcggcgcggg
    23941 cccagcgcgg aagggccagg acggccccgg acgtggcggc cagggcgagg agatctctgc
    24001 ggctgagacg gtctttcatc gttgctcctc cacgggcatc accgagggta cggcagaggg
    24061 gcgcccgggt ttcagccggg gccgatccga acgcggaccg ccctcttccg tatcggaccg
    24121 atttcgagaa tcaggagccc gctctcagct cggcggtgag ctccgccgcc gagaggacgc
    24181 gcacggggct ctccggcctg ccccgaaagt gacggacgtt gtcggtgacg atgacgtccg
    24241 agccggagcg gcgggcgatc tcggcgatgt gggcgtcgta gaggcgcccg ccggcgaccc
    24301 gttctgcgac ggccgacctc aggaacggga ggcgcgcgac tgccggcaga tccacgacgt
    24361 cgagccgccc gaggacttcc tcctcgagca gccgcagcgc gtcggcgggc gccagacgga
    24421 gctcttccgg aaggcgggtc gcgaccgcgt agtactcgag gcagcagtgc cacgccgtcc
    24481 tcgccccgcg aagccggccc tcggcgacgg cgtccatcac ggcctgcgcc gggcggctgg
    24541 agggtccgac gttgatcgtt ccgccgagga ggacgctcgt gtcgaggaag acgcccgtca
    24601 gccgggccaa cggttccgct cctcctcgac cagcgccgcc acgtcgaccg tggggagcgc
    24661 ccttccccgg accgtcggct gcgccacgag tctccggccg cgtcgaacga gcttcggccc
    24721 ggggaccttc cgcacgagcc ggaccgagac gtcgtcgacg aggatctcca cctcggtgcc
    24781 gggcgtcagg ccggcgcgcg cgcggacgga ggccgggatg acgatcctgc ctgccttgtc
    24841 gatggaggcc ttcatgccat cagtatgcca tcgcgctgcc acgcccgcca ttcctgcccc
    24901 acacggcgcc tacgcccggg tcactcccct ttcaccgtca ggacagcccc ggacgagtag
    24961 gcattgaact ccggggcgta catcgactgg accgtcgccg ggccgacccg gaacgtcccg
    25021 gccatgttcg cccggaggcg gtacttgaac gtgtactcgc cgacgggaag ctgctcgaag
    25081 aagaagttcg tcccgctgtc gcggacctcc tcgtaccagg cgatcccgag gtcccacttg
    25141 tagcggctga cggtcgtctc cggctcgaag ccggctccgc gcgggtcgcg caggtggacg
    25201 tactcggcct cgtgcttcgt ccggagcgag atctgcacct ccacctggtc gcccggcttc
    25261 aggaccgtgc cgtcggcgat cggtttcagg acccactctt tccccgtgtt ctcgcggagg
    25321 aagtagcggc gcgacacgtt gaagaagtcg ccccggtcgg cctccgggag cttctcggtc
    25381 gagaagtgcc aggcggcgga ggcaaacgcg aagcccttgc cccccttctc gacggcgatc
    25441 gttgaggtcg tcttcgggtc gacctgggga cccgggatca cgacctggtt cttccttccg
    25501 gtgtagcggt ccggctcgaa cgtgaacgac gtctcccggc cgccgacggc gaccttcatc
    25561 gtctcccgcc cgccgagggc cccctcggcc ttcaggtagt ggacgaggga gtagaggacc
    25621 tcggccgtcg cccgggtcga cttccactgg ttcagcttct tgttcaggaa gagccactgg
    25681 acgaggccgt cgcgcctcgg gtccttcggc gtcagctccg tgagcgtccg aagcgcgaac
    25741 gcgtgcgtct cgatcgtgtc gttgtaccag agccaggagc ggtcctcctg cgcccagaac
    25801 gtcccgagct cctcggtcgt cttcgcggag tccatgacgc tcgcgaagac gagctgggcg
    25861 tccttcatcc ggttcgcccg cttcagcgtc agcgcgaggt agcccttcag gtagggcgag
    25921 tgctccttcc agtgcttgaa ggagaagtcg aggatctcct tccgctccgc ttccgtcagg
    25981 ccgttctcgg accaggaggg gtcggggaag cagctcgcga cgtaattgac gaacgtcagg
    26041 agctcccagc agcagtcctt ccgcatcagg gagaggaggt catcgcggta gtgcttcgcg
    26101 acgtagcccc aggcccgctg gaccatctcc ttcgggacgt cgacgccgaa ctccgtcgcc
    26161 ttcgcgaggc cgtggaggat gtagaccgtc atgtaggggg acggcgggcc gcccggccac
    26221 caggggaaac cgccgatcga ggtctgcgcc ttcgcgagct tcgcgagagc cgcctcccgc
    26281 tccgcctttg cgacacgcgg gtcgaggact ttcgcgaggc cttcccccgc gtccttcccg
    26341 cctttcgcgt cgacgagcca cgggctctcc tcgagcgtca tcttccggtt cgggtcggcc
    26401 gcgtcccacg tctcgaggcg aacgtcccgc ttcgacatct ccttcgccat ggcggcgacc
    26461 gacgggtact tctcgaacat cgaggtgacg atgccggtcg agacgaagcg gttcagggtc
    26521 tgctcggtgc actcgtacgg gtagttgacg aggtacggga gcgcctcgag gacaccgtag
    26581 aagagctggg cgtcgaccgt gacgaccatc tgctcgttga ggagcgtcgg atcgcccccc
    26641 ttcgccaggt cctcgaagcg catctccttc cgctgcccct cgcgaagcgt gacgaagcgc
    26701 gactggacca ggtgcatccg gctcggaagg accggcagcg gccgcagctc gccgtcggag
    26761 aggttcccgg acgtcgccgt gaccttgaag gcgaccgtcc ggacgccggg tggcgcgatg
    26821 aggtggaacg tctcgttcgc accgccgccg gccgggaccg aaaccgcccg gcgggcgtcg
    26881 gcgaccggga ggccgaagcg ggccgtgagg tcctcgttcg tctcggggtc gaggacctgg
    26941 agcacgaggc tccccgcgag cgtgccctcg gaggcgttgt tgacgacgac cttcagctcg
    27001 gcccggtccc cctcgcgcag gaagcgcggg acgtaggggc ggaccatcag ctccttcacg
    27061 ctcttcgtct cgcgcttgag cgaggcgccg cgcaggtcct tcgtcacggc atggagccag
    27121 acgttccacg acgtcaccga gtcggggacg gtgaactcga tcgacgcgga gccgtcggct
    27181 ccggtcagga gctgcggcgt gaagaacgcg gtctcggcga agttgctccg gagggcgggc
    27241 gcctcggccg ccggcgccac gccggcctcc gtccgcgcct cgttcgcgcc ggcgtcctcc
    27301 ttcttcatgt cgaccgacgt cgccatctgc ggggcggcgg cgggcgcggg ggccgggacg
    27361 ccgtcccgct ccaaggccga ctcggccacc atcgccacgc ccattccgcc gcgcatcgcg
    27421 aaccttcgtc cgcgcgcccc gggcccgccg atgccgtacc ggtcgagctc gagcagccgg
    27481 tcgggcgaga acgacgggcc ggacggcgtc ggggcgaagc tgccccggac ccactgtgcc
    27541 cggcccgcac cgagggtcgc ccgcgtccag gccgtccccg tccgggaggg atagagggag
    27601 aggatcgacg gcgggtggtg gggagcgaag acgtcgaggc tccggtcgta catgtacgcg
    27661 aggagctcgg ccgcgccggc ttccaccggc ttcccggagg gtgacgtcac cttcacgcgc
    27721 cacgtctcgc gcgtccctgg gcggatccgg tcgcggaacg tcgcgaattc cagcttcagc
    27781 tcgcggtcgt cccacgggac gaagaccgag gccgtctggc tcagccactg gtgatcgcgg
    27841 agcgccgtca gcgtgacgcc gaagccgccg cggtccgcct ccgttaccgg gaactcgacg
    27901 acggtcgggt ccttccccgc cgtcaggagc gtgcgcgaga ggagcttccc gccgcggaaa
    27961 cgctcgagga
//
LOCUS       DAOONL010000038        27878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1513_length_27878_cov_15.061388, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000038 DAOONL010000000
VERSION     DAOONL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_27878_cov_15.061388"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLP50_07795"
     CDS             <1..413
                     /locus_tag="PLP50_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA51491.1"
                     /translation="AVSSGAFGSVQCSVNVCDQRSLSLVAGGGLGVVAKRPLANTPWN
                     PSAAHPDDAAAAAYRSRWAALHLDDLGLPPAELAVRFATFAPGVSTAIVGSRRLSHLA
                     EMSALVAKGPLPEQMQIEIETRFAALGRDWPGMI"
     gene            complement(1265..2779)
                     /locus_tag="PLP50_07800"
     CDS             complement(1265..2779)
                     /locus_tag="PLP50_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HPA51492.1"
                     /translation="MTTRIRNRALLDRRVSAEEAAGMIRPGDTVGMSGFTGAGYPKAV
                     PVALAKRIMDASLRGERFRVGIWTGASTAPELDGALAMVDGVELRLPYQSDPTCRKKI
                     NAGDMEYLDIHLSHVGAFVRQGFLGKLDVALVEVTGIREDGALVPASSIGNNKVWLDS
                     AERVILEVNSWQSESLEGVHDVYQGFALPPNRKPIPLVGPADRIGTPYLMCPPEKIVA
                     IVETNAPDRNAPFSAPDEASKRIAGHILEFLGGEVKKGRLPKNLLPLQSGVGNIANAV
                     LFGLEDGPFTDLTAYTEVLQDGMLSLIASGKLRVASATAFSLSPQKVDELNAQMDRYR
                     DSLILRPQEISNHPELIRRLGCLAMNGMIEADIYGNVNSTHVNGTAIMNGIGGSGDFA
                     RNAYISFFMTPSTAKKGAVSCIVPMVTHVDHTEHDVHVLVTEQGLADLRGLSPKQRAE
                     RVIEKCAHPDYRPALRDYVRRAGSSSFGRHTPHLLDEAFAWHRQLLRTGTMQGA"
     gene            complement(2817..3383)
                     /locus_tag="PLP50_07805"
     CDS             complement(2817..3383)
                     /locus_tag="PLP50_07805"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HPA51493.1"
                     /translation="MKVSRTFLAALLALPLTAAEPPAPEALASQARGLAGNLMLSLQG
                     ELFAAMKAGGPVKALEVCRSRAPEIASATAMGTPWKIARTSRRVRNGANAPDEWEARK
                     LDEFATRLSAGEEVGALEAWEVVEVEGRPAFRYMKAIGTASPCLTCHGSALKPEIASK
                     VRELYPDDQAVGFVAGELRGAFTLSRPL"
     gene            complement(3414..5972)
                     /gene="hrpB"
                     /locus_tag="PLP50_07810"
     CDS             complement(3414..5972)
                     /gene="hrpB"
                     /locus_tag="PLP50_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HPA51494.1"
                     /translation="MKTTLPIDPFLPEIAATLRRRRALVLVAPPGAGKTTRVPRALLD
                     AGLLESGALWVLQPRRVAARAAARRLAAELGEEVGERVGYQVRDEGAHSERTRILVVT
                     EGILTRRLLEDPALPGISGVMLDEFHERSRHADLALAMLREVRESLREELLLVVASAT
                     LDPEPVSRFLADARGPAPILSVPGRPFPVEVRHAAGADPRPLEVRVASAVSTALGDGP
                     PGDVLAFLPGAAEIRRARETLASLDARLDLRLLHGDLPSAAQDAALAPPADGRRRVVL
                     ATNVAETSLTIEGVRSVVDSGLARTLRFDPRSGLDRLLLGRIPRASADQRAGRAGRLG
                     PGLAIRLFSEHEERGLRPFEEPELLRTDLAAPLLDVLAWSPRDPARFGWFERPPASSV
                     ERALRLLRRLGAVEELSFHPTPLGRRMARLPLHPRLGALVLAGHSAGLARQAATLAAL
                     VEERDVLAGSRAFGRAVAAPEHDSDLLLRLELLEEAERAGLSRPRLESLGLDPGAAHA
                     VLRLRDALLRRLPSSPRPPRASEEELLRLVLHGWPDRVARRRRGRPSEAVLAGGTPLA
                     FDPGCTVRSADLVVVVETVAGTNGPDRIRVASAVERAWLDALPGAGVLSETVRSWDAA
                     AGRVVATQRAVFDGLVLEEDEIPAPADEETARLLLEAASADPASVLALDGPAERLRAR
                     VAFLRRAMPELELPPLDDDALRELLGELVPGRRRLSELRAVPLEPLLRARLGARALRL
                     LDLHAPATLPVPTGRDLPLAYGGDGPPVLAVKLQELFGLARTPSVAQGRVPVLLHLLS
                     PAGRPVQVTTDLASFWTRTYPEVRKELRGRYPKHPWPDDPRSAPPRRGTTRSGR"
     gene            6061..6246
                     /locus_tag="PLP50_07815"
     CDS             6061..6246
                     /locus_tag="PLP50_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51495.1"
                     /translation="MTTDASVDPRLSPAARGGGDAHHELMDDVVGDEESSKTQRWIGW
                     LCGLLTLAAAVALWRLT"
     gene            6551..7834
                     /locus_tag="PLP50_07820"
     CDS             6551..7834
                     /locus_tag="PLP50_07820"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPA51496.1"
                     /translation="MKTPFNPFEMAQAQFDKAADVLGLDPATRDLLRFPLREYHVAIP
                     VRMDDGSVKVFRGFRVQHNDSRGPCKGGIRFHPHETADTVRALAMWMTWKCAVVDIPL
                     GGGKGGVICDPHHLSAREQEGICRGWIRQVAKNVGPVNDVPAPDVMTTPQHMLWMLDE
                     YETLAGGKYPGFITGKPVGSGGSLGRTEATGYGVIFTLREALKELGIDITKTKASVQG
                     FGNVAQYAIKLYTQLGGKVVCCSSWDQADQTSYSFRRLSGVDADELLGITDRFGGIDK
                     DKAKGLGYEVLPGDAWIEQDVEILIPAALENQVTGETVKKIKPSVKVIAEGANGPTTP
                     EADKVIQERGIYVIPDFLANAGGVTCSYFEQVQCNMNYFWEKDEVLQKLDTKMTSAFH
                     AVPELAKKRNLYMRDAAYVIAVSRVAKACKDRGWA"
     gene            7997..11248
                     /locus_tag="PLP50_07825"
     CDS             7997..11248
                     /locus_tag="PLP50_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPA51497.1"
                     /translation="MPGTGRSLIEELQERAKELQCLYRVHAICARAEAPLDELLREVA
                     SVLPAGWEHPAETVVRIVVGSSVYVSAEAEPSPWVQSAPIRVQGQEAGRLEVSYREPF
                     PVSDEGPFRREERKLIDTVAELLGQLLLQRRLSAAIRKAEKSGLPLPAEGGDWWVIVD
                     FLRQIDRPGYVTIARRMLNYLCWSGDEEAMSLLPRFTGSWSGPAGPEEPTDENRPMAR
                     RNLDDLLKVAEEVFVLASARMAPGEIRTSIQRWSKDSRSGFLIEALENQGTSLTELAE
                     ALTRFRHAALNDNELSRSIQFDLRAALVRRFLTDQLDFVGVAKNFIGVSDFQELTRRI
                     ICPPGSHGKVGGKSSGLFLAVQIVRKSEEYAETLADIRIPKTWYVTSDGVVNFIAYNH
                     LEDIVDRKYLEIDQVRREYPHIIQVFKNSQFSPEIVKGLSVALDDFEERPIIVRSSSL
                     LEDRAGASFSGKYKSLFLANQGPKRDRLAALMDAIAEVYASIFGPDPIEYRAERGLLD
                     VHEEMGIMIQEVVGSRVGKYWLPTFAGVAFSNNEFRWSPRIRRDDGLIRLVPGLGTRA
                     VDRVGDDYPILLAPGQPGLRANVTADAVVRYAPRMADVINLETRQFETVEMETLFREA
                     GGSLPGIDEMVSVHEEGRIHRPLMVDYQERARDLVVTFEGLVTGTPFVARIRALLKLL
                     QEKLGSPVDVEFASDGKDLYLLQCRPQSFADESQAAVLPTDVPPERVVFSANRFVSNG
                     RVPDVTHVVYVDPESYASIPSLDTLREVGHAVGRLNKLLPRRQFILMGPGRWGSRGDI
                     KLGVAVTYSEINNAAVLIEIARRKGSYVPDLSFGTHFFQDLVEAQIRYLPLFPDDPGV
                     DFNERFLLDSPNLLPTLVPEAAHLAKTLRVIDVPAATGGLVLKVRMNADLDRAVAILA
                     PPGAPEKGAGGVRQAAIGIPGSAGADDDRRWRIHLAERIAAETDPERFGVKGMWLVGS
                     SHDGTAGPGSDVDLVVHTDDDPARREALATWLEGWSLCLGEVNYLRTGLRTKGLLDVR
                     FISDSDISRGTSWAAKVTGAKDPARPLPLHASVRTGPVPRKGRGAGKNEGGPR"
     gene            11245..12108
                     /locus_tag="PLP50_07830"
     CDS             11245..12108
                     /locus_tag="PLP50_07830"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPA51498.1"
                     /translation="MNASALPLAAAGLALAALAPTAAAQPRLPFPSPAASVSQEIGTS
                     RVTVEYHRPAVRSRAIWGALVPYGETWRLGANEATTLTLSHDGKVAGHDVPAGTYALF
                     AVPGKERWTLVLNRKAKQWGAYFHDPKEDLLRFEVTPAAAPHAEWMTFALDAVEPASV
                     RVSWAWETLAFSFPVEFDVPGIVWAGVDAALKEKPDDMGTLLQAARWALQRKERLAEG
                     RAWIDRSVAVKESFWNRELLANYLRHEGKTAEAVVAMRRAIELSKGKAPDGYGKALEA
                     TVAAWEGEKAP"
     gene            12105..12617
                     /locus_tag="PLP50_07835"
     CDS             12105..12617
                     /locus_tag="PLP50_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HPA51499.1"
                     /translation="MSGGAPPGLFPEEAGGRFLDVGVLDALSTEARSRPRRRANGNLH
                     AMEDPVHRLLNAVEPGTYIRPHRHLHPPKDETYLVVRGALGLVLFDASGAVAATRRLS
                     AAAGEAFGVDLPAGVLHTVVALEEGTVFFEVKAGPYAPSCGDDVPPWAPAEGEPAATA
                     YEARLRALFP"
     gene            complement(12693..15785)
                     /locus_tag="PLP50_07840"
     CDS             complement(12693..15785)
                     /locus_tag="PLP50_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51500.1"
                     /translation="MARLSRSRVRWASTVLTVVLALGAATAAIAQETTARLFGAVTMK
                     SDQSPLPGVSVEAIHVPTGTRYTALTQANGRYTILNARVGGPYTITATISGFRPSTQK
                     DISLALGEGRQVNFGLDLESVTETVVVTAEGTPLISPDRMGSTSAMAEEQIKTLPTVR
                     RQLQDFARLNPYVGVDASDQTGTRISVAGKNNRYNTIQIDGAVNNDLFGLADTGTPGG
                     QTDAQPISLDAIQELQVVVSPFDIKQGGFTGGGINAITRGGTNEFHGSAYGSLRDQSF
                     VGDGPLDRPISDFDEDQYGFRIGGPILRDRLFFFLNGEMNRREAPTGVSADGTTTTVF
                     NDPAAAERFRNFLISRYDYDPGGLGDFGQKTDSDNAFVRLDFNVNESHNATLRHNYVD
                     AARDVVGDRSSTRFRFPTGIYAIADETNSTVLQVNSVFGSSSFNVARVGYQTIRDVRD
                     TPVIFPTVDIGPIARQPQLSAGTERFSGANSLDQDILEITDDFTFVKGNHTITVGTHN
                     EIFDFKNLFLSDFYGWYRFNTVADFEAGIAADYSITFANGADPRRPTAFGAEQWGLYV
                     GDNWRVNDKLTLSLGVRADMPRIPDSPARNQAVYDTFGIDTSDVPSGDVTVSPRLGFN
                     FAIDPKQQVRGGIGVFAGRTPFVWISNAFGGTGIETTSLSARNVPFNPDPFNQPKNFP
                     PGSSAVSVDGIDPDFEFPRVWRATLAYDRELPFGVRGSLEGMYTETIQDVYYMNMNKV
                     ATGNVAFDGRPTYTNVASGFRDIPYMTNTSLGRQTNVTLQLEKRFDFGLALTGSYAWT
                     DAKAGFEGTSSRAISNWQFHATRGDIFSPELSRSFWEIEDRFNITASQNFRTGPLAHN
                     VALFYTVQSGTPYSVMMGGDPNRDGYSSNDLLYVPNNASDIVLQGATAAEFDAFLAWT
                     GLDAYRGQVVARNSSFTPWNRSLDFHYDVELPISVVRTQLTFDVLNLINLIDSEKGII
                     RYVSNQSYTPLNYQGIDSASGKPIYRTAFNGALGEGRAFSINDLRSRWQFKFGLRLSF
                     "
     gene            16028..17209
                     /locus_tag="PLP50_07845"
     CDS             16028..17209
                     /locus_tag="PLP50_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA51501.1"
                     /translation="MPTAEAAAPVVPPLSILTESEREIAENVHQFAAETIGPRSLAMD
                     LANRMDPELVRQFFDLDLMGIEIPEEFGGLGQNFTTSIAVIEAIARVDAACAVCVDVQ
                     NTLVDNAVLRWGSPELRAKYLPQLAKQKVGSYALSEAESGSDAFALKTRAEKKGDRWV
                     LNGGKLWITNGLEAELFLVFANVDFSKGYKGITAFVVESGWKGFTKGKKEDKLGIRAS
                     STLALAFEDLEVPEENVLGKVGEGYKIAIETLNEGRIGIGAQMLGVARAALDYSAKYV
                     QTRVQFGKPISEFQAIQHELARMDMELECARLAVYNAARLKEAGKPFAREGAIAKLKA
                     SEVAEYCTSKGLELLGGYGYVREYPLEKMYRDAKIGKIYEGTSYMQLSTIAKDVLKRN
                     A"
     gene            17265..17340
                     /locus_tag="PLP50_07850"
     tRNA            17265..17340
                     /locus_tag="PLP50_07850"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17298..17300,aa:Cys,seq:gca)
     gene            17433..17517
                     /locus_tag="PLP50_07855"
     tRNA            17433..17517
                     /locus_tag="PLP50_07855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17466..17468,aa:Leu,seq:taa)
     gene            complement(17634..18296)
                     /locus_tag="PLP50_07860"
     CDS             complement(17634..18296)
                     /locus_tag="PLP50_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51502.1"
                     /translation="MKIPLPDSILGPNTFADFEAAPGSSGADLLVYVGGAVSPSEYHA
                     RRESSPTALLTLLRPDGARHSALALPYSPTLPGRPEDAWKAFLDHFLHEVLPALPIRP
                     DRMAVIGYSLGAALAVLLARAGGPRVVRLATVGAVGAVEAFLLEAPPLGPLICPVHVA
                     WNTEDPCSTHSLRFAGALEAAGVRHDVDTTTGGHAFPDYVANGRLPAAVAFAAEGLST
                     SC"
     gene            complement(18604..18903)
                     /locus_tag="PLP50_07865"
     CDS             complement(18604..18903)
                     /locus_tag="PLP50_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51503.1"
                     /translation="MNRNQALAWELHRQRPMPVDVRERDVLDDAIQAAHIREEMNEKK
                     LPSPTAPQQQTKSVESSATSSRTEASSPAEERVYQRLLRHSRIKAALAREDLPVN"
     gene            18979..19371
                     /locus_tag="PLP50_07870"
     CDS             18979..19371
                     /locus_tag="PLP50_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51504.1"
                     /translation="MKLTLAVVIASLALVGCGSTGVLPVGNASALPKPEGCQVAVFDS
                     EEDVGRPFEKLCIIDAKTGSTLYNDRSVSGAMKRVNAAACQCGADAVIVKAMGKQGVS
                     AWDWGSSKVSVVAIRYTGPAEASPAGSD"
     gene            19532..19729
                     /locus_tag="PLP50_07875"
     CDS             19532..19729
                     /locus_tag="PLP50_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51505.1"
                     /translation="MTEAEVRLFIGFLEAKAEQYWAHHAAARSQHHDSAHSFKFEAKT
                     YEDVHRSLLWILDGRDPMTGR"
     gene            19910..20476
                     /locus_tag="PLP50_07880"
     CDS             19910..20476
                     /locus_tag="PLP50_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51506.1"
                     /translation="MLAAVLVLTQAVAATPSPVPSTPTPAPTSEASPASPPSATRKLS
                     GGFGRKPEAPGKTKVVLTDETLPPTRKAGTFSVSGTRTPAKTGVPVEGEGSEPPVKAG
                     DEATWKARVATLRAALAQAEKDYDVADKANTVVAFGEAGNDYQTMMAIRNAALTPYRM
                     KLDELRSALAALPEECRKTPGCQPGWVR"
     gene            20571..23468
                     /locus_tag="PLP50_07885"
     CDS             20571..23468
                     /locus_tag="PLP50_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HPA51507.1"
                     /translation="MKPEEAARRQIDAMLEAAGWSVQDAGSVNLSASRGVAIREFPLA
                     HGYGFADYLLYVDGRAAGVIEAKKEGTPLTGVELQAERYAHGVSEAVPAAVRPVPFRY
                     LSTGVETRFANHLDPEPRSRNLFHFHRPELLATWLGADPLPTPGGKYPTGAPRPGTLR
                     GHLKAMPPLNGTGLWPAQVRAVENLEKSLAEDRPRALIQMATGSGKTFTAITSVYRLV
                     KHAGARRVLFLVDRANLGRQAHKEFQQYTTPDDGRKFTELYNVQLLSSNRIDPVARVV
                     ITTIQRLYSMLRGEPDLDPSLEEGSQFATGGGLVSEPVPVAYNAAIPVETFDVVFIDE
                     CHRSIYSLWRQVLEYFDATLVGLTATPSKQTFGFFNQNLVMEYGHAQAVADGVNVDYD
                     VYRIRTRITENGSTIEAGDDETIGKRDRATRALRWERLDEDITYAGGDLDRDVVALDQ
                     IRTVVRTFRDRLFTEIFPGRSEVPKTLIYAKDDSHADDIVQIVREEFGKGNEFAEKIT
                     YRTGTARVVAKTTGPDGAKTEDVTYKATGVKAEDLLSSFRNAYNPRVVVTVDMIATGT
                     DIKPLEVVMFLRAVKSRNFFEQMKGRGVRVITPTEFQQVTPDASNKDRFVLVDCVGIC
                     ESDLVDTQPLEKKRSMSFEKLLQAVAFGSTDPALLSSLASRLARIDHRIGEPEREALK
                     EAAGGVSLGHLAGSLVEALDPDRQVEAARVANGLPPGAEPTVEQVQKAATALLVEAAK
                     PVASNPALRNTLLSVQRSLDQTIDDISKDEVLEAGYSEAAREKAHALVTSFEEFLAEN
                     RDEITALQVLYSRPYRMRLKPEDVKALVEKLKAPPRAWTPELLWRAYETLERDKVRGA
                     ESRRLLTDVVSLVRFAFHQEDELVPFRNRVEERYSAWLAAQEARGVAFTDEQRRWLDD
                     IRDHIAANLAIEMDDFEYVPFAQKGGAGKVYQLFGEKLPGLLEELNEVLAA"
     gene            23541..23834
                     /locus_tag="PLP50_07890"
     CDS             23541..23834
                     /locus_tag="PLP50_07890"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPA51508.1"
                     /translation="MPTNLHIDDALLTRVMELGRFKTKREAVNEALKSKLDLLNRQEL
                     LAAVGTIDFDSSYDRKADARRHAAKMVRLGVWPKDALIPEAEAPKPKKRPARR"
     gene            23831..24247
                     /locus_tag="PLP50_07895"
     CDS             23831..24247
                     /locus_tag="PLP50_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPA51509.1"
                     /translation="MIVLDASVLSTGLGRRPRLVGREDPVAREVARILASAEPYFVPG
                     IVAQEYLSGAQSPEQERFLDDVVAKFAIRPATAEEHRIAARISNECRWRGFAVGSADC
                     LIAAQTLAADGFLFTLDKDVERIAPLCGLRLWRAGR"
     gene            24414..25943
                     /locus_tag="PLP50_07900"
     CDS             24414..25943
                     /locus_tag="PLP50_07900"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPA51510.1"
                     /translation="MTVPLGNVLREALRNGHSAKASSDGRGIRTLTLTAVTVGDFSEC
                     NTKVTVADPAKVRDLWLESGDLLVERANTPELVGTARLYRGPSGYAIFPDLLIRVRLS
                     EGVSPSFVELALSTDSARSHFRRSAQGIAGSMPKIDQQTIEEFPLVLAPEPEQRRIVD
                     EVEKQFTRLDAAVAGLQRAKANLKRYRASVLKAACEGRLVPTEAELARREGRSFESGE
                     ELLKRILKERRARWEADQLAKMTAAGRVPSPDGWKAGYREPRKPEIDGVPLPKGWAYS
                     TVETVGDVLLGRQRAPQYQTGRWTRPYLRVANVKDDRLDFRDVETMDFDPVHFEKYRL
                     VPGDILVSEGQSPERVGESAVYRGGIDGLCFQKTLHRFRPVPGGPSSGFAQIVFRTHV
                     KTGVFMRLASITTNIAHLTLEKFEAAPFPLPPLPEQHRIVAEVERRLSVVDELEATVE
                     KNLARCARLRQSILKMAFEGRLVPQDPNEEPASVLLDRIRERRGPEEPRRRGVRRVPR
                     P"
     gene            complement(25921..26325)
                     /locus_tag="PLP50_07905"
     CDS             complement(25921..26325)
                     /locus_tag="PLP50_07905"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPA51511.1"
                     /translation="MAAPRPPKLLLDECCGGSLAVALRARGATVVEAGFDRALPRGTP
                     DEEVLRWAGANGFAFLTRDRHLKTRPAELAAIKAHRVAVFYVPPSPRSREELCKAVTA
                     ALPRIARCLEKENRPFVRRILPNGALKDEELA"
     gene            complement(26303..27010)
                     /locus_tag="PLP50_07910"
     CDS             complement(26303..27010)
                     /locus_tag="PLP50_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPA51512.1"
                     /translation="MRTPVLDPRSQPAYTLREAALCVRVPPATLRTWTAGRPYPTATE
                     RRHSDPLILLADGDRGLFSFVNLIEAQVLRALRTVHELSLQNVRKALREYGRHDPKDH
                     PLAFEDFQTDGLDLFVKRYGQTINLSRSGQIALLSTLEQHLQRVDRDGIGPIRLFPFV
                     RTGADRRDLVSVSPLVAFGRPVIEGTRVGTDDVVARIRRGDSPADVAEDLHLTEQQVI
                     DACVFEEWDRRGGAQAA"
     gene            27176..>27878
                     /locus_tag="PLP50_07915"
     CDS             27176..>27878
                     /locus_tag="PLP50_07915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPA51513.1"
                     /translation="MSSNGSQQVVQRLWNYCNVLRDDGLSYGDYVEQLTFLLFLKMAH
                     EQTQPPWSRPSPIPEGFAWPSLLKRDGDALESHYRHLLETLGKEKGMLGLIFRKAQNK
                     VQDPAKLRRLVVDLIDPQQWMTLDADVKGDAYEGLLEKNAQDTKGGAGQYFTPRALIR
                     AMVEVMRPQPGETIADPACGTGGFLLAAHDYIQAANPHLEKAQKKHLKHQALRGVELV
                     ASVSRLCAMNLLLHGI"
BASE COUNT         4408 a         9491 c         9925 g         4054 t
ORIGIN      
        1 gggccgtctc ctccggcgcc ttcggctccg tccagtgctc ggtcaacgtc tgcgatcagc
       61 gctccctctc tctcgtcgcg ggcggcggcc tcggagtcgt cgccaagcgt cccctcgcga
      121 acacgccctg gaacccctcc gccgcccatc ccgacgacgc ggccgccgcg gcctaccgct
      181 cccgctgggc cgcccttcac ctcgacgacc tcggcctccc tcccgccgag ctcgccgttc
      241 gcttcgccac cttcgccccc ggagtctcga ccgcgatcgt cggctcccgc cgactctccc
      301 acctcgccga gatgagcgcc ctcgtcgcga agggcccgct ccccgagcag atgcagatcg
      361 agatcgaaac ccgcttcgcc gccctcggtc gcgactggcc gggaatgatc tgaggcggga
      421 agagggcgac cggaagccgg agcacgagaa cacgagagca cgagagatga ccgggcagag
      481 ccgacttcgt tcctcagaag tcgcggccag gaggggcatc ccacggcgct cgccgggggg
      541 cctgagcttc gcgcggcccc ccggaccccc cactcgcaat ctcggcatcg ctgccgttga
      601 gaggcctccg ctgcgcgcgc gcgacccgca tcggcgcctc tgcgcgggcc atcggcgcgg
      661 ccgcgaactc ctcgcgctcg ccggggggct gagcttcgcg cgccccccgg acccccacat
      721 tcgctcggag caacatcgct cgtcacatgc cgagctcgtc aaacagcgcg tccgcttttc
      781 ccgatggcct gctcgcggag ccgtgcgggt accccgcgcg tgctcgaacg ctcgctcgcg
      841 ggacgcccct cccgtccgct cacgccacgg gtggatcagc gaagaggaaa tcgaaggcga
      901 ggctcggggc tgttcgggga agtcggaggt ctggtcggtc cccgaggatg accctcaccc
      961 ctgctcggaa ctgacagggc ggtcctttcc gcgctcttcg gtcctgctcc ggtgggccga
     1021 gccacgggat gggtcccgcg agccgcctcg agcacggccg gggacccgct cgcgatcgcg
     1081 aggaccgggc ggggatcagg cccgcgcgct gtttgagcga agcgagttcg cgcgggcccc
     1141 cacccggccg cagcgggcgc gaaagcgggt cggccgcgcg cagccagcgg ccgtgggacc
     1201 catcccgggg cgaggctccc tccctcgtgc cccggtgctc tcgtgctccc gcgctccggt
     1261 gccctcaggc tccctgcatc gtgccggtgc ggaggagctg gcggtgccag gcgaaggcct
     1321 cgtcgaggag gtgcggcgtg tggcggccga atgaggagct tcccgcgcgc ctcacgtagt
     1381 cgcggagggc ggggcggtag tcggggtggg cgcacttctc gatgacgcgc tcggcgcgct
     1441 gcttcggcga gaggccgcgc aggtcggcga ggccctgctc ggtgacgagg acgtggacgt
     1501 cgtgctcggt gtggtcgacg tgcgtgacca tcgggacgat gcaggagacg gctcccttct
     1561 tggcggtcga gggagtcatg aagaaggaga tgtaggcgtt gcgcgcgaag tcgcccgagc
     1621 cgccgatgcc gttcatgatc gcggtgccgt tcacgtgcgt cgagttcacg ttgccgtaga
     1681 tgtcggcctc gatcatcccg ttcatcgcga ggcagccgag gcggcggatc agctcggggt
     1741 ggttcgagat ctcctggggc cgcaggatca ggctgtcccg gtagcggtcc atctgcgcgt
     1801 tcagctcgtc gaccttctgc gggctgaggg agaaggcggt cgcggaggcg acgcgcagct
     1861 tccccgaggc gatgagcgag agcatcccgt cctggaggac ctcggtgtag gccgtcaggt
     1921 cggtgaacgg gccgtcctcc aggccgaaga ggaccgcgtt cgcgatgttc ccgacgcccg
     1981 actggagggg caggaggttc ttcgggagcc gccccttctt cacctcgcct ccgaggaact
     2041 cgaggatgtg ccccgcgatc cgcttcgacg cctcgtcggg cgccgagaac ggggcgttgc
     2101 ggtcgggcgc gttcgtctcg acgatcgcga cgatcttctc gggcgggcac atcaggtaag
     2161 gcgtgccgat ccggtccgcc ggcccgacga gcgggatcgg cttgcggttc ggcgggagcg
     2221 cgaagccctg gtagacgtcg tggacgccct cgaggctctc cgactgccag gaattgacct
     2281 ccaggatgac gcgctcggcg gagtcgagcc agaccttgtt gttcccgatc gaggaggcgg
     2341 ggacgagcgc gccgtcctcg cggatgccgg tcacctcgac gagcgcgacg tcgagcttcc
     2401 cgaggaaacc ctgcctcacg aacgccccga cgtgcgagag gtggatgtcg aggtactcca
     2461 tgtcgcccgc gttgatcttc ttccggcagg tcgggtcgga ctggtagggg agcctcagct
     2521 ccacgccgtc gaccatcgcg agcgcgccgt cgagctcggg ggcggtcgac gcgcccgtcc
     2581 agatcccgac gcggaatctc tcgccccgga ggctcgcgtc catgatccgc ttcgcgaggg
     2641 cgaccgggac ggccttcggg tagccggctc ccgtgaaacc gctcatcccg acggtgtcgc
     2701 cgggccggat cattccggcg gcctcctcgg ccgagacgcg ccggtcgaga agagcgcggt
     2761 tccggatgcg cgtcgtcatg gcgtcggtcc tccttcgagc cggtcggcgt ccgcgatcag
     2821 agcgggcggg agagggtgaa ggcgcctcgg agctctccgg ccacgaagcc gacggcctgg
     2881 tcgtccgggt agagctcccg caccttcgac gcgatctcgg gcttgagcgc cgagccgtgg
     2941 caggtgaggc agggcgaggc ggtgccgatc gccttcatgt agcggaacgc cggccttcct
     3001 tcgacctcga cgacctccca ggcctccagg gcgccgacct cctcgcccgc ggagagccgc
     3061 gtcgcgaact cgtcgagctt gcgtgcctcc cactcgtccg gcgcgttcgc gccgttgcgg
     3121 accctgcgcg acgtgcgggc gatcttccag ggggtcccca tcgcggttgc cgaggcgatt
     3181 tcgggcgcac ggctccggca gacctcgagc gccttcaccg ggccgccggc cttcatcgcg
     3241 gcgaagagct caccctggag cgagagcatc aggttgccgg cgaggccgcg cgcctgagag
     3301 gcgagcgcct cgggagcggg gggctcggcc gcggtgagag ggagcgcgag aagggcggcg
     3361 aggaacgtgc gcgagacctt catctcttcc tccgtcggga ggagggtagc gcgtcagcgg
     3421 ccggagcggg tcgtgccgcg ccgcggcggg gcgctgcggg ggtcgtcggg ccaggggtgc
     3481 ttcgggtagc gcccccgcag ctccttccgc acctcggggt atgtgcgggt ccagaagctc
     3541 gcgaggtcgg tggtgacctg gacgggacgc cccgcggggg agaggaggtg aaggaggacc
     3601 ggaactcgcc cctgggccac gctcggcgtc cgggcgagac cgaagagctc ctggagcttc
     3661 acggcgagga ccggcggccc gtcgcctccg taggccagcg gaaggtcccg gccggtcggc
     3721 acggggagcg tcgccggcgc atggagatcg aggaggcgga gagcgcgggc gccgagccgg
     3781 gcgcgcagga gcggctcgag agggaccgcg cgtagctcgg agagccgccg gcggccgggg
     3841 acgagctcgc cgaggagctc tcggagggcg tcgtcgtcga ggggtggcag ctccagctcg
     3901 ggcatcgccc gccggaggaa cgcgacgcgt gcgcggagac gctccgccgg tccgtcgagc
     3961 gcgaggacgg acgccgggtc ggcgctcgcc gcctcgagga ggagccgcgc ggtctcctcg
     4021 tcggcgggcg cgggaatctc gtcctcctcg aggacgaggc cgtcgaagac ggctctctgc
     4081 gtcgcgacga cgcggccggc ggcggcgtcc caggagcgga ccgtctccga gaggacgccc
     4141 gcgccgggca gcgcgtcgag ccacgcgcgc tccaccgcgc tcgcgacccg gatccggtcg
     4201 gggccgttcg ttcccgcgac ggtctcgacg acgacgacga ggtcggcgct ccgaacggtg
     4261 cagcccgggt cgaaggcgag cggagtcccg ccggcgagga ccgcctccga cgggcggccg
     4321 cgcctcctcc gagcgacccg gtccggccac ccgtggagga cgagccggag gagctcctcc
     4381 tcggaggcgc ggggaggcct cggtgaggag gggagtcgcc ggaggagcgc gtcccgcagg
     4441 cgcaggacgg cgtgggcggc ccccggatcg agcccgagcg actcgaggcg cggccgggag
     4501 aggcccgccc gctcggcctc ctcgaggagc tcgaggcgga gcaggaggtc ggagtcgtgc
     4561 tccggcgccg cgacggcccg gccgaaggcg cggctccccg cgaggacgtc gcgctcctcg
     4621 acgagcgccg cgagcgtcgc cgcctgacgc gcgagcccgg ccgaatggcc cgcgaggacg
     4681 agggccccga ggcgcgggtg aagcgggaga cgcgccatgc ggcggccgag cggggtcgga
     4741 tggaacgaga gctcctcgac cgctcccagc cgccggagga ggcggagcgc cctctcgacg
     4801 gacgaggcgg gcggcctctc gaaccagccg aagcgggccg ggtcgcgagg cgaccaggcg
     4861 aggacgtcga ggagaggagc ggcgaggtcg gtccggagga gctcgggctc ctcgaacggg
     4921 cgcaggccgc gctcctcgtg ctcggagaag aggcggatcg cgaggccggg gccgaggcgc
     4981 ccggcgcggc cggcgcgctg gtcggcgctc gcgcgcggga tccggccgag gaggaggcgg
     5041 tcgaggccgg agcgcgggtc gaagcggagc gtccgcgcga ggccggagtc gacgacggag
     5101 cggaccccct cgatcgtgag ggaggtctcg gcgacgttcg tcgcgaggac gacgcgacgg
     5161 cgtccgtcgg cgggcggtgc gagcgccgcg tcctgggcgg cggaggggag gtcgccgtgg
     5221 aggagccgga ggtcgaggcg cgcgtcgagc gacgcgagcg tctcgcgcgc ccggcggatc
     5281 tcggccgctc cggggaggaa ggcgaggacg tcccccggcg gcccgtcgcc gagggccgtc
     5341 gagactgcgg aagcgacccg gacctcgagg gggcggggat cggcgccggc cgcgtgccgg
     5401 acctcgaccg gaaaggggcg gcccggcacg gagaggatcg gcgccgggcc ccgcgcgtcg
     5461 gcgaggaacc gcgagacggg ctccgggtcg agcgtcgcgg aggcgacgac gaggaggagc
     5521 tcctcgcgga gcgactcgcg gacctcgcgg agcatcgcga gggcgaggtc ggcgtggcgg
     5581 ctccgctcgt ggaactcgtc gagcatcacg ccggagatcc cggggagcgc gggatcttcg
     5641 aggaggcggc gggtcaggat cccctcggtg acgacgagga tgcgggtccg ttccgaatgc
     5701 gccccttcgt cgcggacctg gtagccgacc ctctccccga cttcctcgcc cagctcggcg
     5761 gcgaggcgcc gcgcggcggc ccgggccgcg acccggcgag gctggaggac ccagagggca
     5821 cccgactcga ggaggcccgc gtcgagcagc gcccgcggaa cgcgggtcgt ctttccggcg
     5881 cccggagggg cgacgaggac gagcgcgcga cgccgccgga gcgtcgcggc gatctcggga
     5941 agaaacgggt cgatcgggag cgttgtcttc accaggggcg gacaggatac ggggcgtcgg
     6001 ccggcgccgt cccggagagg agacgacgtc gcaccgaagg cccgggaacg ggaggctgtg
     6061 atgacgacgg acgcttcggt tgacccccgg ctctcgccgg ctgcccgcgg cggcggcgac
     6121 gcgcaccacg agctcatgga cgacgtcgtc ggcgacgagg agtcgtcgaa gacccagcgt
     6181 tggatcggct ggctctgcgg cctgctcacc ctcgccgccg cggtcgccct ctggcgcctg
     6241 acctgagccg cccgaggtcc cgtcgggtcc gagcggtcgc cgcggccggc ccctccccgc
     6301 tcgaccgggt gcgaggtccg aggagagggg ccccgcgacc aggtcgggcg ccgcgccgga
     6361 aacggccttc gtgcggctcg gaaagccctc cgcggtgcta gaatttctcg ttgatggccc
     6421 gccggttcgc ggctccgttc tcccggattc caggcgcgga gcggaggcgt attccgggtc
     6481 cgctcgcgaa cagggaagac ccggcccacc acaaaccgcc caccacccac gagaggagat
     6541 tcgtcgttcc atgaagactc cgttcaaccc cttcgagatg gcccaggcgc agttcgacaa
     6601 ggcggccgac gtcctcggcc tcgacccggc cacccgcgac ctccttcgct tccccctccg
     6661 cgagtaccac gtcgcgatcc cggtccggat ggacgacggg agcgtgaagg tcttccgcgg
     6721 tttccgcgtc cagcacaacg actcccgcgg cccctgcaag ggcggcatcc gcttccatcc
     6781 gcacgagacg gccgacacgg tccgcgccct cgcgatgtgg atgacctgga agtgcgcggt
     6841 cgtcgacatc ccgctcggcg gcggcaaggg cggcgtcatc tgcgacccgc accacctgag
     6901 cgcgcgcgag caggagggga tctgccgcgg ctggatccgc caggtcgcca agaacgtcgg
     6961 ccccgtcaac gacgtcccgg cgcccgacgt catgacgacc ccgcagcaca tgctctggat
     7021 gctcgacgaa tacgagacgc tcgcgggcgg caagtacccc ggattcatca ccggaaagcc
     7081 ggtcggctcg ggcggctcgc tcggccggac cgaggcgacg ggctacggcg tcatcttcac
     7141 gctcagggag gcgctcaagg agctcgggat cgacatcacg aagacgaagg cctccgtcca
     7201 gggcttcggc aacgtcgcgc agtacgcgat caagctctac acgcagctcg ggggcaaggt
     7261 cgtctgctgc tcgtcgtggg accaggccga ccagacctcc tactccttcc gccggctctc
     7321 gggcgtcgac gccgacgagc tcctcggcat caccgaccgc ttcggcggga tcgacaagga
     7381 caaggcgaag ggtctcggct acgaggtcct ccccggcgac gcctggatcg agcaggacgt
     7441 cgagatcctc atcccggccg ccctcgagaa ccaggtgacg ggcgagacgg tcaagaagat
     7501 caagccgagc gtgaaggtca tcgccgaggg cgccaacggg ccgacgacgc ccgaggccga
     7561 caaggtgatc caggagcgcg gcatctacgt catccccgac ttcctcgcga acgccggcgg
     7621 cgtcacctgc agctacttcg agcaggtgca gtgcaacatg aactacttct gggagaagga
     7681 cgaggtcctc cagaagctcg acacgaagat gacctccgcc ttccacgccg tccccgagct
     7741 cgcgaagaag cgcaacctct acatgcgcga cgcggcctac gtcatcgcgg tctcccgcgt
     7801 ggccaaggcc tgcaaggatc gcggctgggc ctgatcggtt ccccggccgg gggagctccg
     7861 cgaggggctc ccccggcctt ccccctcgtt ccggccgccc gctcctcccg cctcgggcgg
     7921 cgtccccgct gaccgctccc tgaacgaccg gccctgtggc actctcgcgc ggcacggcga
     7981 gccgcaggag gtgtcgatgc ccggtacggg tcggtccctg atcgaggagc tccaggagcg
     8041 ggcgaaggag ctccagtgtc tctaccgggt ccatgcgatc tgcgcgcggg cggaggctcc
     8101 gctcgacgag ctgctccggg aggtcgcgag cgtcctgccg gcgggctggg agcacccggc
     8161 cgagaccgtc gtccggatcg tcgtcggctc ctccgtctac gtttcggccg aggccgagcc
     8221 gagcccctgg gtgcagtccg cgccgatccg ggtccagggg caggaggcgg ggcgcctcga
     8281 ggtctcctat cgcgagccgt tcccggtgtc ggacgagggg ccgttccggc gggaggagcg
     8341 aaagctcatc gacacggtcg ccgagctcct cggccagctc ctcctccagc gacggctcag
     8401 cgcggcgatc cgcaaggccg agaagagcgg tctccccctt ccggccgagg ggggcgactg
     8461 gtgggtcatc gtcgacttcc tgcggcagat cgaccgcccc ggctacgtga cgatcgcgcg
     8521 gcgaatgctc aactacctct gctggagcgg cgacgaggag gcgatgagcc tccttccgcg
     8581 cttcacgggg agctggagcg ggccggcggg ccccgaggag ccgaccgacg agaaccgccc
     8641 gatggcgcgc cggaacctgg acgatctcct gaaggtggcg gaggaggtct tcgtcctcgc
     8701 ctcggcccgg atggcgccgg gcgagatccg gacgagcatc cagcgctgga gcaaggacag
     8761 ccgctccggg ttcctgatcg aggccctcga gaaccagggg acgtcgctca cggagctcgc
     8821 ggaggcgctg acgcgcttcc ggcacgccgc gctgaacgac aacgagctct cccggtcgat
     8881 ccagttcgac ctgcgcgcgg cgctcgtgcg gcggttcctc acggaccagc tcgacttcgt
     8941 gggcgtcgcg aagaacttca tcggcgtctc ggacttccag gagctgacgc ggcggatcat
     9001 ctgcccgccc gggagccacg ggaaggtcgg cggcaagagc tcggggctct tcctcgccgt
     9061 ccagatcgtc cgcaagtcgg aggagtacgc cgagacgctc gccgacatcc ggatcccgaa
     9121 gacgtggtac gtgacgtcgg acggcgtcgt gaacttcatc gcctacaacc acctcgagga
     9181 catcgtcgac cgcaagtacc tcgagatcga ccaggtgcgg cgcgagtacc cgcacatcat
     9241 ccaggtcttc aagaacagcc agttctcgcc ggagatcgtc aaggggctct ccgtcgcgct
     9301 cgacgacttc gaggagcggc cgatcatcgt gcggagctcg agcctcctcg aggaccgcgc
     9361 cggcgcctcg ttctcgggga agtacaagag cctcttcctc gcgaaccagg ggccgaagag
     9421 ggaccgcctg gccgcgctca tggacgccat cgccgaggtc tacgcctcga tcttcggccc
     9481 cgacccgatc gagtaccgcg ccgagcgcgg cctcctcgac gtccacgagg agatggggat
     9541 catgatccag gaggtcgtcg ggtcgcgggt cgggaagtac tggctcccga cgttcgccgg
     9601 cgtcgccttc tcgaacaacg agttccgctg gtcgccccgc atccggcgcg acgacggcct
     9661 gatccggctg gtcccgggcc tcggcacgcg cgccgtcgat cgcgtcggcg acgactaccc
     9721 gatcctcctc gcccccgggc agccaggcct ccgcgcgaac gtcaccgccg acgccgtcgt
     9781 ccgctacgcg ccccggatgg cggacgtcat caacctcgag acccgccagt tcgagacggt
     9841 cgagatggag accctcttcc gcgaggcggg cgggagcctg ccgggcatcg acgagatggt
     9901 ctcggtccac gaagagggac ggatccaccg cccgctcatg gtcgactatc aggagcgggc
     9961 tcgggacctc gtcgtcacgt tcgagggcct cgtcaccggg acgccgttcg tcgcgaggat
    10021 ccgggcgctc ctcaagctcc tgcaggagaa gctcggcagc ccggtcgacg tcgagttcgc
    10081 gtcggacggg aaggacctct accttctcca gtgccggccg cagagcttcg ccgacgagag
    10141 ccaggcggcc gtcctcccga ccgacgtccc tccggagcgg gtcgtcttct cggcgaaccg
    10201 attcgtctcg aacggacgcg tccccgacgt cacgcacgtc gtctacgtcg acccggagtc
    10261 ctacgcgtcg atcccgagcc tcgacaccct ccgcgaggtc gggcacgccg tcgggcggct
    10321 gaacaagctc ctcccgcggc ggcagttcat cctgatgggg ccgggacgtt gggggagccg
    10381 cggcgacatc aagctgggcg tcgcggtgac ctactccgag atcaacaacg cggccgtgct
    10441 gatcgagatc gcgcgccgca aggggagcta cgtcccggac ctctccttcg gcacccactt
    10501 cttccaggac ctcgtcgagg cccagatccg ctacctcccg ctcttcccgg acgaccccgg
    10561 ggtcgacttc aacgagcgct tcctgctcga ttccccgaac ctcctgccga cgctcgtccc
    10621 cgaggccgcg cacctcgcga agactctccg tgtcatcgac gtcccggcgg cgactggcgg
    10681 cctcgtcctg aaggtgagga tgaacgccga cctcgaccgg gccgtcgcga tcctcgcccc
    10741 gccgggagcg ccggaaaagg gggcgggagg ggtccgccag gccgcgatcg gcatccccgg
    10801 ctcggccggc gcggacgacg accggcgctg gaggattcac ctcgccgagc ggatcgccgc
    10861 cgagaccgac ccggagcgct tcggcgtgaa ggggatgtgg ctcgtcggct cctcgcacga
    10921 cggcacggcc gggccgggga gcgacgtcga cctcgtcgtc cacaccgacg acgaccctgc
    10981 ccggcgcgag gccctcgcga cgtggctcga agggtggagc ctctgcctcg gcgaggtgaa
    11041 ctacctccgg acggggctcc ggacgaaggg gctcctcgac gtccgcttca tctccgactc
    11101 ggacatctcc cgcgggacga gctgggccgc gaaggtgacg ggagcgaagg acccggcgcg
    11161 gcccctgccc ctccacgcgt cggtccggac cggccccgtg ccccggaagg ggcgtggcgc
    11221 cgggaagaac gaaggaggcc cccgatgaac gcaagcgccc ttccgctcgc cgccgccggc
    11281 ctcgcgctcg cggcgctcgc cccgaccgcc gcggcccagc cgcgcctccc gttcccgagc
    11341 cccgcggcct cggtctccca ggagatcggc acctcgcgcg tgacggtcga gtaccaccgg
    11401 cccgccgtcc gcagccgcgc gatctggggc gccctcgtcc cgtacggcga gacgtggcgc
    11461 cttggcgcga acgaggcgac gacgctgacc ctctcccacg acgggaaggt cgccgggcac
    11521 gacgtgccgg ccggcacgta cgccctcttc gccgtcccgg ggaaggagcg gtggacgctc
    11581 gtcctgaacc ggaaggcgaa gcagtggggc gcctacttcc acgacccgaa ggaagacctc
    11641 ctccggttcg aggtgacccc tgccgccgcc cctcacgcgg agtggatgac cttcgcgctc
    11701 gacgccgtcg agcccgcgtc cgtgagggtc tcctgggcct gggagacgct cgcgttctcc
    11761 ttcccggtcg agttcgacgt ccccgggatc gtctgggccg gcgtcgacgc cgcgctgaag
    11821 gagaagccgg acgacatggg gacgctcctg caagccgccc gctgggcgct ccagaggaag
    11881 gagcggctcg cggagggacg ggcctggatc gatcgctccg tcgccgtgaa ggagagcttc
    11941 tggaaccgcg agctcctcgc gaactacctt cggcacgagg ggaagacggc cgaggcggtc
    12001 gtcgcgatgc gccgggccat cgagctttcg aaggggaagg cccccgacgg gtacgggaag
    12061 gctctcgagg cgaccgtcgc ggcgtgggag ggggagaagg ccccgtgagc ggaggagcgc
    12121 cgcccgggct ctttcccgag gaggcgggcg gccgcttcct cgacgtcggc gtcctcgacg
    12181 cgctctcgac agaggcccgg agccggccgc gccgcagggc caacgggaac ctccacgcga
    12241 tggaggaccc ggtgcaccgg ctcctcaacg ccgtcgagcc cggcacctat atccggcccc
    12301 accggcacct tcacccgccg aaggacgaga cctacctcgt cgtccggggc gccctcggcc
    12361 tcgtcctctt cgacgcgtcg ggcgcggtgg cggcgacccg tcggctcagc gcagcggccg
    12421 gggaggcgtt cggcgtggac ctgccggcgg gcgtcctcca caccgtcgtc gcgctggagg
    12481 aggggacggt cttcttcgag gtgaaggccg gcccctacgc cccgtcgtgc ggcgacgacg
    12541 tgccgccgtg ggcgcccgcg gaaggcgagc ccgcggccac ggcgtacgag gcgcggctgc
    12601 gggcgctctt cccttagcgc gcgggagcgg cgagccaaag aagaaggggg gcctttcggc
    12661 cccccttctc gatcggactc ccgtccggaa ggtcagaagg agaggcgcag cccgaacttg
    12721 aactgccagc gggagcgcag gtcgttgatc gagaaggcgc ggccctcccc gagcgctccg
    12781 ttgaacgccg tccggtagat cggcttgccc gaggccgagt cgatcccctg gtagttgagg
    12841 ggcgtgtagg actgattcga cacgtaccgg atgatcccct tctcgctgtc gatcaggttg
    12901 atcaggttga ggacgtcgaa cgtcagctgg gtcctcacga cgcttatcgg aagctcgacg
    12961 tcgtagtgga agtcgagcga ccggttccag ggcgtgaagc tcgaattccg cgccacgacc
    13021 tggccgcggt acgcgtcgag gccggtccac gcgaggaagg cgtcgaattc cgcggcggtc
    13081 gctccctgga ggacgatgtc gctcgcgttg ttcggcacgt agaggaggtc gttcgaggag
    13141 tagccgtccc ggttcgggtc tccgcccatc atgacggagt acggcgtgcc ggactggacc
    13201 gtgtagaaga gggccacgtt gtgggccagc gggccggtcc ggaagttctg ggaagccgtg
    13261 atgttgaagc ggtcctcgat ctcccagaag gagcgggaga gctcggggct aaagatgtcg
    13321 ccgcgggtgg cgtggaactg ccagttcgag atcgcgcggc tcgaggttcc ctcgaacccg
    13381 gccttcgcgt cggtccaggc gtaggagccg gtgagggcga ggccgaagtc gaacctcttt
    13441 tcgagctgga gcgtcacgtt cgtctgccgc ccgagcgagg tgttcgtcat gtacgggatg
    13501 tcgcggaagc ccgacgccac gttcgtgtag gtcggccggc cgtcgaacgc gacgttgccg
    13561 gtcgcgacct tgttcatgtt catgtagtag acgtcctgga tcgtctcggt gtacatcccc
    13621 tcgagcgagc cgcggacccc gaaggggagt tcacggtcgt aggcgagggt ggcgcgccag
    13681 acgcggggga actcgaagtc cgggtcaatg ccgtcaacgg agacggcgga ggagccgggc
    13741 gggaagttct tcggctggtt gaacggatcg gggttgaagg ggacgttgcg ggcggagagg
    13801 ctcgtcgtct cgatgccggt gccgccaaag gcattcgaga tccagacgaa cggggtgcgg
    13861 cccgcgaaga cgccgatgcc gccacggacc tgctgcttcg ggtcgatcgc gaagttgaag
    13921 ccgaggcgcg gggagacggt gacgtcaccg ctcgggacgt cgctcgtgtc gatgccgaac
    13981 gtgtcgtaga cggcctggtt ccgggccggc gagtcgggga tgcgcggcat gtcggcgcgg
    14041 accccgaggc tgagcgtcag cttgtcgttg acgcgccagt tgtcgcccac gtagaggccc
    14101 cactgctcgg cgccgaaggc ggtcgggcgg cgcgggtcgg cgccgttcgc gaaggtgatc
    14161 gagtagtccg cggcgatgcc ggcctcgaag tccgcgacgg tattgaaccg gtaccagccg
    14221 tagaagtcgg agaggaacag gttcttgaag tcgaagatct cgttgtgggt cccgaccgtg
    14281 attgtgtggt tgcccttgac gaacgtgaag tcgtcggtga tctcgaggat gtcctggtcg
    14341 agcgagttcg cgcccgagaa gcgctcggtc ccggccgaga gctgcggctg gcgcgcgatc
    14401 gggccgatgt cgacagtcgg gaagatgacc ggcgtgtcgc ggacgtcacg gatcgtctgg
    14461 tagccgacgc gggccacgtt gaacgagctc gagccgaaga cgctgttcac ctggaggacc
    14521 gtcgagttcg tttcgtcggc gatcgcgtag atgcccgtcg ggaagcggaa gcgcgtgctc
    14581 gagcggtcgc cgacgacgtc tcgggcggcg tcgacgtagt tgtgccggag cgtcgcgttg
    14641 tgcgactcgt tcacgttgaa gtcgagacga acgaacgcgt tgtcgctgtc ggtcttctga
    14701 ccgaagtcgc cgaggccgcc cggatcgtag tcgtacctcg agatcaggaa gttccggaac
    14761 cgctcggcgg cggcggggtc gttgaacacc gtcgtcgtcg taccgtccgc ggaaacgccg
    14821 gtcggcgcct cgcgccggtt catctcaccg ttgaggaaga agaagagccg gtccctcagg
    14881 atcgggccgc cgatgcggaa gccgtactgg tcctcgtcga agtccgagat cggacggtcg
    14941 agagggccat cgccgacgaa gctttggtcg cggagtgagc cataggcgga gccgtggaac
    15001 tcgttcgtgc caccgcgggt gatggcgttg atgccaccgc ccgtgaagcc gccctgcttg
    15061 atgtcaaacg gcgagacgac gacctgaagc tcctggatcg cgtcgaggct gatcggctgg
    15121 gcgtcggtct ggccgcccgg cgtgccggtg tcggcgaggc cgaacaggtc attgttgacg
    15181 gcgccgtcga tctggatcgt gttgtaacgg ttgttctttc cggccacgct gatgcgcgtg
    15241 cccgtctggt cgctcgcgtc gaccccgacg taggggttca gccgggcgaa gtcctgcagc
    15301 tggcgccgga ccgtcgggag cgtcttgatc tgctcctccg ccatggccga cgtcgagccc
    15361 atccggtccg ggctgatgag cggggtcccc tcggccgtga cgacgaccgt ctcggtgacg
    15421 gactcgaggt cgagcccgaa gttcacctgg cggccctcgc cgagggcgag ggagatgtcc
    15481 ttctgggtgg aggggcggaa gccgctgatc gtcgcggtga tcgtgtacgg tccgccgacg
    15541 cgggcgttca ggatcgtgta acggccgttc gcctgggtga gcgccgtgta tcgcgtcccc
    15601 gtcggaacgt gaatcgcctc gaccgagacg cccggaagcg ggctctggtc ggacttcatc
    15661 gtgaccgcgc cgaagaggcg cgccgtggtc tcctgagcga tcgccgctgt ggcggcgccc
    15721 agggcgagga cgaccgtcag gacggtcgac gcccaacgga cccgggaacg actgagtctt
    15781 gccatatggc ctttcctcct cctcaaatcg attgaggaca cgtacatcct gtcaggaagc
    15841 acacccgatg attgtgaatt cgatgaaaaa gctttgaaac ggcgagcgag gaaaatcagg
    15901 cacccccaga gcggctccgt ggagccctcc gcaagccgcg ggggagtcta taggagcggg
    15961 aaggggagcg tcaacgacgg aaaggggtcg gagtttcgtt ctatcctcgc ctcgcaagga
    16021 gagactgatg cccacagccg aagcggcagc ccccgtcgtc ccgcccctgt cgatcctgac
    16081 ggagtccgag cgggagatcg ccgagaacgt ccaccagttc gccgccgaga cgatcggccc
    16141 ccgctccctc gccatggacc tcgcgaaccg gatggacccc gagctcgtcc ggcagttctt
    16201 cgacctcgac ctgatgggga tcgagatccc ggaggagttc ggcggcctcg ggcagaactt
    16261 cacgacctcc atcgcggtca tcgaggcgat cgcgcgcgtc gacgccgcct gcgcggtctg
    16321 cgtcgacgtc cagaacaccc tcgtcgacaa cgcggtcctc cggtggggct cgccggagct
    16381 gagggcgaag tacctgccgc agctcgcgaa gcagaaggtc ggctcgtacg ccctctcgga
    16441 ggcggagtcg ggctccgacg ccttcgcgct gaagacgcgc gccgagaaga agggggaccg
    16501 ctgggtcctg aacggcggca agctctggat cacgaacggc ctcgaggccg agctcttcct
    16561 cgtcttcgcg aacgtcgact tctcgaaggg gtacaagggg atcaccgcct tcgtcgtcga
    16621 gtcgggctgg aagggcttca cgaaggggaa gaaggaggac aagctcggta tccgcgcctc
    16681 ctccacgctc gccctcgcct tcgaggacct ggaggtcccg gaggagaacg tcctcgggaa
    16741 ggtcggcgag gggtacaaga tcgccatcga gacgctgaac gaggggcgga tcgggatcgg
    16801 cgcccagatg ctcggcgtcg cccgcgcggc cctcgactac tcggcgaagt acgtccagac
    16861 gcgcgttcag ttcggcaagc cgatctccga gttccaggcg atccagcacg agctcgcccg
    16921 gatggacatg gagctcgagt gcgcccgcct cgcggtctac aacgccgccc gcctgaagga
    16981 ggcggggaag ccgttcgccc gcgaaggggc gatcgcgaag ctgaaggcct cggaggtcgc
    17041 ggagtactgc acctcgaagg ggctcgagct cctcggcggc tacggctacg tgcgggagta
    17101 cccgctcgag aagatgtacc gcgacgcgaa gatcgggaag atctacgagg ggacgtccta
    17161 catgcagctc tcgacgatcg cgaaggacgt cctgaagcgg aacgcctgat cccagcgggg
    17221 cgtctggccc ggacagccgg ccgctgctag aatccgcctc cctgggcgac gtacccaagt
    17281 ggttaaggga aggctctgca aaagcctcat tcgtcggttc gaatccgacc gtcgcctcca
    17341 gactgaaacc gccgccgcgt ctccgggcgc ggcggttctc tttgaggggc cgggcgcctc
    17401 ccggtctacg atcgaccgtc cgggacgacc gggccggagt ggcggaatgg cagacgcagg
    17461 ggccttaaaa gccccgggcc ccgaagggcc atgagggttc gagtccctcc tccggcaatg
    17521 acttacgagg aaacgcccgg aatcgtcccg gcgaaggttg cccacacgta gcccaagcgc
    17581 ctcggaaaca aggagggcca gccccggggc tctgcgcctc tcccggggcg gcgtcagcag
    17641 gacgtcgaga gcccctctgc cgcgaacgcc acggcggccg gcaggcggcc gttcgcgacg
    17701 tagtccggga aggcgtgtcc gcccgttgtg gtgtcgacgt cgtgccggac tccagccgcc
    17761 tccagcgccc ccgcgaaccg gagcgaatgc gtcgagcatg gatcctctgt gttccacgcg
    17821 acgtgcaccg gacagatgag cggcccgagg gggggcgcct ccagcaggaa cgcctctacc
    17881 gccccgaccg cccccacggt cgcaagccgg accacccgcg ggccgccggc gcgcgcgagg
    17941 aggaccgcga gagcagctcc gagggaatac ccgatgacgg ccatacggtc gggccggatc
    18001 ggcagagctg gcagcacctc gtgaaggaag tgatcgagaa acgccttcca ggcatcttcg
    18061 ggtcggcccg ggagcgtggg agagtagggg agcgcgagcg ccgagtgacg cgctccgtcc
    18121 ggtcgaagga gagtcaggag agcggtcggc gaggactctc gccgggcatg gtactcactc
    18181 ggggagacgg caccaccgac gtagacgaga agatctgcac cagacgaccc gggggcggcc
    18241 tcgaaatcgg cgaaggtgtt cggccccagg atggaatctg gtagcgggat cttcatggtc
    18301 cgccgtcgat gagttcacgg gacctcgaga tcgaaaggta ctcgatgcct cgcgatcacc
    18361 gcgaccgctt cgaggaaggt ttcgccgagc ccgcgggagc tgcatcggag cctttcgagc
    18421 tgtcggcgcg tcctggctcg agctcggaca agtcgaagaa cagtccctcc tgtgctaccc
    18481 ggtagcggaa gggattcacg tcgaggaacg gcttcgtcca gagtcgggat acctcccgaa
    18541 gcgcagggtt cttgctggtg cggagtatgt cgaatcggcc gatcatcccg tccatctccg
    18601 agctcagttc acagggagat cttcccgggc gagggcggcc ttgatccggc tgtgtcgcag
    18661 cagtcgctgg tagaccctct cttccgcggg ggagctcgct tccgtgcgcg acgaagtggc
    18721 ggacgattcg actgacttcg tctgctgttg cggggccgtc ggtgaaggaa gcttcttctc
    18781 gttcatctcc tcccgaatgt gggctgcttg gatcgcatcg tcaaggacgt cgcgctcccg
    18841 cacgtcgacg ggcatcggtc gttgccgatg gagctcccat gcaagcgcct ggttccggtt
    18901 cactcgagtc gggcttcctt cgtcaacacg gacaccatct gtccgtgtgc ggtcgtacgg
    18961 tctccatcgg aggagctcat gaagctgacc cttgctgtcg taatcgcctc cctcgcgctc
    19021 gtcggatgcg ggtcaacggg cgttcttcct gtcggcaacg ccagtgctct gccgaagccc
    19081 gagggatgcc aggtcgccgt cttcgactcc gaggaagacg tcggccggcc cttcgagaag
    19141 ctctgcatca tcgacgcgaa gaccggttcg accctctaca acgacaggtc cgtctccggg
    19201 gccatgaaga gggtgaacgc ggcggcctgc cagtgcggag ctgacgccgt catcgtgaag
    19261 gccatgggga agcaaggagt ctccgcctgg gactgggggt cttcgaaggt ctctgtcgtc
    19321 gcgatccggt acaccggccc ggccgaggct tcgccggcgg ggtcggactg accgctgctg
    19381 cggggtctga cgccggcgcg gacgagcttc ggagcgcagc cggaggtcga gatcgtccgg
    19441 gctgaatcga tgatccaggg tgacggatgg agacgggagg gactcagtgg cagagtcttc
    19501 gtacctaccg gaggggatcc agccgcgagc catgacggag gccgaggtgc ggctcttcat
    19561 cggcttcctg gaggcgaagg cggagcagta ttgggcgcac cacgctgcgg cgaggagtca
    19621 gcaccacgac tcggcccaca gcttcaagtt cgaggcgaag acctacgagg acgttcaccg
    19681 atcgcttctg tggatactgg acggacggga cccgatgacg gggcggtgaa cggaggggag
    19741 ccttcgttga agaccactcg tgctccctct cgcactgcaa gcaggaggaa gccggaagac
    19801 gaaccgaagc tgccctgcgg tcgcacgtcc gagtgtgccg gccccgcccc ccgggacacc
    19861 attcgtccgt atctcacctt cagccttttc gacggaggag tccatcgcca tgcttgcagc
    19921 cgtcctcgtc ctaacccagg ccgtcgccgc cacgccgagt cccgtcccat caacgcccac
    19981 gccagcgcct acgtccgaag cgtccccggc ttcgccgccc tcggcgaccc ggaagctctc
    20041 gggcggcttt ggaaggaagc cggaggcccc tggcaagacg aaggtcgtcc ttaccgacga
    20101 gaccctcccg cccacgcgga aggccgggac attcagcgtc tcgggcacgc ggaccccggc
    20161 gaagaccggc gtcccggtgg aaggagaagg ttcggagcct cccgtaaagg cgggcgacga
    20221 ggccacctgg aaggctcgag tcgcgacgct ccgcgcggcg ctggcgcagg ccgagaagga
    20281 ctacgacgtc gctgacaagg ccaacaccgt cgtcgccttc ggggaggcgg ggaatgatta
    20341 ccagacgatg atggcgatcc gaaacgccgc tctcacgccg taccggatga agctcgatga
    20401 gctccggtcg gcgctcgcgg cccttcccga ggagtgccgg aagacgcccg gatgtcagcc
    20461 cggatgggtg cggtagcgga cctcacgtct tatcctcggc gtcgagagtc ccgaatcgct
    20521 cctcccgtct gacgacggcg agcgtcgggg gctgaaacac cgaggggggc atgaagccgg
    20581 aagaggccgc gcgaaggcag atcgacgcga tgctcgaggc ggcgggctgg tccgtccagg
    20641 acgccggctc cgtcaacctc tccgcctccc gaggcgtcgc catccgcgag ttccccctcg
    20701 cccacggcta cggcttcgcc gactacctcc tctacgtcga cggcagggcc gccggcgtca
    20761 tcgaggccaa gaaggagggg actcccctca ccggcgtcga gctccaggcc gagcggtacg
    20821 cccacggcgt ctcggaggcc gtcccggcag ccgttcgccc cgtccccttc cgctacctct
    20881 cgacgggcgt cgaaacccgc ttcgcgaacc acctcgaccc cgagccgcgc agccggaacc
    20941 tcttccactt ccaccgtccc gagctcctcg ccacctggct cggggccgat cccctcccca
    21001 cacccggcgg caagtacccg accggggcgc cccgcccggg caccctccgg ggccacctga
    21061 aggccatgcc acccctgaac ggaaccggcc tctggcccgc ccaggtccgg gccgtcgaga
    21121 acctcgagaa gtccctcgcc gaagaccggc cccgcgcctt gatccagatg gcgaccgggt
    21181 cggggaagac cttcacggcc atcacctcgg tctaccgcct cgtgaagcac gccggggcgc
    21241 gccgggtcct cttcctcgtc gaccgcgcga acctcggtcg ccaggcccac aaggagttcc
    21301 agcagtacac gacgccggac gacggccgga agttcaccga gctctacaac gtccagctcc
    21361 tcagctcgaa ccggatcgac ccggtcgccc gcgtcgtcat caccaccatc caacggctct
    21421 actcgatgct ccggggcgag ccggacctcg acccctcgct cgaggagggc tcccagttcg
    21481 cgacgggggg cgggctcgtc tccgagccgg tcccggtcgc ctacaacgcc gcgatccccg
    21541 tcgagacctt cgacgtcgtc ttcatcgacg agtgccaccg ctcgatctac tccctctggc
    21601 ggcaggtcct cgagtacttc gacgccaccc tcgtcggcct cacggcgacc ccgtcgaagc
    21661 agaccttcgg cttcttcaac cagaacctcg tcatggagta cggccacgcc caggccgtcg
    21721 ccgacggcgt caacgtcgac tacgacgtct accgcatccg gacccggatc accgagaacg
    21781 gctcgacgat cgaggcgggc gacgacgaga ccatcggcaa gcgggaccgg gccacccgcg
    21841 ctctccgctg ggagcggctc gacgaggaca tcacctacgc cggcggcgac ctcgaccgcg
    21901 acgtcgtggc cctcgaccag atccggaccg tcgtccggac gttccgcgac cgcctcttca
    21961 ccgagatctt cccgggccgg agcgaggtgc cgaagaccct catctacgcc aaggacgaca
    22021 gccacgccga cgacatcgtc cagatcgtcc gggaggagtt cggcaagggg aacgagttcg
    22081 cggagaagat cacctaccgc accggcacgg cccgcgtcgt cgcgaagacg accggcccgg
    22141 acggcgccaa gaccgaggac gtcacctaca aggcgaccgg ggtgaaggcc gaggacctcc
    22201 tctcctcctt ccggaacgcc tacaaccccc gcgtcgtcgt cacggtcgac atgatcgcca
    22261 ccggcaccga catcaagccg ctcgaggtcg tcatgttcct tcgggcggtg aagagccgga
    22321 acttcttcga gcagatgaag gggcgcggcg tccgcgtcat caccccgacg gagttccagc
    22381 aggtcacccc cgacgcctcg aacaaggacc gcttcgtcct cgtcgactgc gtcggcatct
    22441 gcgagagcga cctcgtcgac acccagccgc tcgagaagaa gcggtcgatg tcgttcgaga
    22501 agctcctcca ggcggtcgcg ttcggaagca cggaccctgc cctcctctcc tccctcgcga
    22561 gccgcctcgc ccgcatcgac caccggatcg gtgagcccga aagggaggcg ctgaaggagg
    22621 cggctggggg cgtgagcctc gggcatctcg ccggaagcct cgtggaagcc ctggaccccg
    22681 accggcaggt cgaggcggcc cgggtcgcga acgggctccc gccgggggcg gaaccgaccg
    22741 tggagcaggt gcagaaggct gcgacggctc tcctcgtcga ggcggcgaag cccgtcgcct
    22801 cgaatcctgc tctccggaac accctcctct ccgtccagag gtcgctcgac cagacgatcg
    22861 acgacatctc gaaggacgag gtcctcgagg cgggctactc cgaagccgcc cgggagaagg
    22921 cccacgccct cgtcacgtcg ttcgaggagt tcctcgccga gaaccgggac gagatcaccg
    22981 cgctccaggt cctctacagc cggccttacc ggatgaggct gaagcctgaa gacgtcaagg
    23041 cgctcgtcga gaagctcaag gccccgcccc gggcatggac gccggagctt ctctggcgtg
    23101 cctacgagac cctcgagagg gacaaggtcc ggggggccga atcccgccgc ctcctcaccg
    23161 acgtcgtctc gctcgtccgg ttcgcgttcc accaggagga cgagctcgtc ccgttccgga
    23221 acagggtcga ggagcgctac tcggcgtggc tcgccgcgca ggaggcgcgg ggcgtcgcct
    23281 ttaccgacga acagcgacgg tggctcgacg acatccggga ccacatcgcc gcgaacctgg
    23341 cgatcgagat ggacgacttc gagtacgtcc ccttcgcgca gaagggcggg gccggaaagg
    23401 tctaccagct gttcggggag aagctgccgg ggctgctcga ggagctgaac gaggtcctcg
    23461 cggcctgacg tcgtcgccct ccgcgggcga tgtgtggaag aatgtgtgag gctcctggcc
    23521 gggggcgccg gaggtggacc atgccgacga atctccacat cgacgacgcg ctgctgaccc
    23581 gggtgatgga gctgggtcgc ttcaagacga agcgcgaggc ggtgaacgag gcgctgaagt
    23641 ccaagctgga cctcctgaac cgccaggagc tcctggcggc tgtcgggacg atcgacttcg
    23701 actcgagcta cgaccggaag gccgacgccc gccgccacgc cgcgaagatg gtccggctcg
    23761 gggtctggcc gaaggacgcg ttgatccccg aggcggaggc tccgaagccg aagaagcggc
    23821 cggcgcgccg gtgatcgtcc tcgacgcctc cgtcctttcg actggcctgg ggcgacgtcc
    23881 gaggctcgtc ggacgcgagg atcccgtcgc gagggaggtg gcgcgcatcc tcgcgtcggc
    23941 ggaaccgtac ttcgtcccgg gaatcgtcgc gcaggaatac ctctcggggg cgcagagccc
    24001 cgagcaggag cggttcctcg acgacgtcgt cgcgaagttc gcgattcgtc cggcgacggc
    24061 tgaggagcac cggatcgcag cccggatctc gaacgagtgc cgctggcgcg gcttcgcggt
    24121 cgggtccgcg gattgcctca tcgccgccca gacgctcgcc gccgacgggt tcctcttcac
    24181 gctcgacaag gacgtcgaac gcatcgcgcc gttgtgcggc ctgaggctgt ggagggcggg
    24241 gcgatgaggg gcggggcggg gatctgcccg cggcgaggca gcgaggacct atctgtgcca
    24301 cccgaggacc tgaatgaggt gatcgcggcg tgagagctct gtcgcgagag atggagcgca
    24361 atgacccagc cggttccgag aaggcccggt caggccccct gccggaaggc tgggtgacgg
    24421 ttcccctcgg aaacgtgctt cgggaagcgc tccgaaacgg ccattcagcg aaggcgtctt
    24481 cggacggccg aggcattcgg accctgaccc taaccgctgt tacggtcggt gacttctcgg
    24541 agtgcaacac aaaagtgacg gtcgccgacc cagcaaaggt gcgagaccta tggctcgaat
    24601 cgggcgatct actcgtcgaa agggcgaata caccggaact cgtcgggacg gcccggctct
    24661 atcgaggtcc gagtggctac gcgatcttcc ctgaccttct gatcagggtc cgcctctccg
    24721 aaggcgtttc tccgtccttc gtggagctag cactttcgac ggactcggcg cggtcgcact
    24781 ttcggcgctc ggctcaagga attgcaggca gcatgccgaa gatcgaccag cagacgatcg
    24841 aagagttccc actcgtcctc gcccccgagc ccgagcagcg caggatcgtc gacgaggtcg
    24901 agaagcagtt cacgcggctc gacgccgccg tggcgggcct ccagcgggcc aaggcgaacc
    24961 tcaaacgcta ccgggcgtcg gtcctgaagg ccgcctgcga ggggcgcctc gtgccgaccg
    25021 aggccgagct ggcacggcgg gaggggcgtt cgttcgagtc gggcgaggag ctgctgaagc
    25081 gaatcctgaa ggagcgccgc gcccgctggg aggccgacca gctcgcgaag atgacggcgg
    25141 cggggagggt gccatcgccg gacgggtgga aggcgggata tcgcgaaccc cgtaagccgg
    25201 agatcgacgg agtacccctg cccaaaggct gggcctactc aaccgtcgag accgtcggtg
    25261 atgtgttgct gggtcgccag cgagcaccgc aataccagac ggggcggtgg acccggcctt
    25321 atctgcgcgt cgcgaacgtg aaggacgatc ggctggactt cagagacgtg gaaacgatgg
    25381 actttgatcc ggtccacttc gagaagtacc gactggttcc gggcgacatc ctggtgagcg
    25441 agggtcaaag ccctgaacgg gtcggcgaga gcgccgttta ccgcggtggg atcgacggcc
    25501 tctgctttca gaagacgctc caccgcttca ggcccgttcc cggcggccct tcatcaggat
    25561 ttgctcagat cgtgtttcgg acgcacgtga agaccggtgt cttcatgcgc ctggcgtcga
    25621 tcacaacgaa tatcgcgcac ctcacgctgg agaagttcga ggccgcacct tttcccctgc
    25681 cccccctccc cgagcagcat cgcatcgtcg ccgaggtcga gcgccgcctc tccgtcgtcg
    25741 acgagctcga ggcgaccgtc gagaagaacc tcgctcgctg tgcgcgcctg cgccagtcga
    25801 tcctgaagat ggccttcgag gggcgcctcg tccctcagga cccgaacgaa gagccggcga
    25861 gcgtcctcct cgatcggatc cgagaaaggc gggggcctga agagccgcgg cgccgaggtg
    25921 tcaggcgagt tcctcgtcct tgagcgcgcc gttcgggagg attcgccgga cgaaggggcg
    25981 gttctccttt tcgaggcagc gagcgattcg aggaagcgcg gccgtcacgg ccttgcagag
    26041 ctcttcgcgc gagcgcgggc tcggggggac gtagaagaca gcgacgcggt gggccttgat
    26101 cgccgcgagc tcggcgggcc gggtcttcag atggcggtcg cgcgtgagga acgcgaagcc
    26161 gttcgctccc gcccatcgga gcacctcctc gtcgggggtg cctcggggga gtgcccggtc
    26221 gaacccggcc tcgacgacgg tcgctcctcg ggcgcgaagc gccacagcga gcgaaccgcc
    26281 gcagcattcg tcgaggagga gcttaggcgg cctgggcgcc gccacgccgg tcccattcct
    26341 cgaagacgca ggcgtcgatg acctgctgct cggtgaggtg gaggtcctcg gcgacgtcgg
    26401 cgggggagtc accgcgccgg atccgggcga cgacgtcgtc ggtaccgacg cgcgtcccct
    26461 cgatcaccgg tcggccgaac gcgacgagcg gggagacgga cacgagatcg cgtcggtcgg
    26521 cgccggtgcg gacgaacggg aagagacgaa tcggaccgat gccgtccctg tccaccctct
    26581 gcaggtgctg ctcgagagtg gagaggaggg cgatctggcc ggaacggctc aggttgatgg
    26641 tctggccgta ccgcttcacg aacagatcca gtccgtcggt ctggaagtcc tcgaaggcga
    26701 gcggatggtc cttggggtcg tgcctgccgt actcgcgaag cgccttgcgg acgttctgga
    26761 ggctgagctc gtggacggtt cgaagcgccc tgagaacctg ggcctcgatg aggttcacga
    26821 aggaaaagag gccccggtcg ccgtcggcca gcaggatgag cggatcggag tgccgccgtt
    26881 cggtcgcagt cggatacggc cggccggccg tccaggtgcg aagcgttgcc ggcggaacgc
    26941 ggacgcagag cgcggcctcg cgaagggtgt aggccggctg ggaccgcgga tcgagaacgg
    27001 gcgttcgcat ggagaccgtg gcggcattat cgcctgagcg gaagggcggc tcccgcccgt
    27061 ctgttaccag atgcctcctg caaacgaggg gtcgtcacac agcaaacttc agcgaaccag
    27121 agacttacga tctcggtcat tatcaggggg cccgattcgg gcggagagag ggcgcatgtc
    27181 gagtaacggc tcccagcagg tcgtccagcg cctctggaac tactgcaatg tcctgcgcga
    27241 cgacggtctc tcgtacggcg actacgtcga gcagctgacg ttcctcctct tcctgaagat
    27301 ggcgcacgag cagacgcagc cgccctggag ccggccgtcg ccgatcccgg aaggcttcgc
    27361 ctggccgagc ctcctgaagc gggacggcga cgcgctggag tcgcactacc ggcaccttct
    27421 cgagacgctc ggcaaggaga aggggatgct gggcctgatc ttccggaagg cccagaacaa
    27481 ggtccaggac ccggcgaagc tccggcggct cgtcgtcgac ctcatcgacc cgcagcagtg
    27541 gatgaccctc gacgccgacg tgaagggcga cgcctacgag ggtctcctcg agaagaacgc
    27601 ccaggacacg aaagggggcg ccgggcagta cttcacaccg cgggcgctca tccgggcgat
    27661 ggtcgaggtg atgcgccccc agccgggcga gacgatcgcc gacccggcct gcggcacggg
    27721 cggcttcctc ctcgccgcgc acgactacat ccaggccgca aacccccacc tcgagaaggc
    27781 ccagaagaag cacctgaagc accaggcgct gcgaggcgtc gagctcgtcg cctccgtgtc
    27841 gcgcctctgc gccatgaacc tcctcctgca cggcatcg
//
LOCUS       DAOONL010000039        27066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1601_length_27066_cov_19.285587, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000039 DAOONL010000000
VERSION     DAOONL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_27066_cov_19.285587"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLP50_07920"
     CDS             <1..334
                     /locus_tag="PLP50_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA51514.1"
                     /translation="DERMAYYDTEELWFPEWENGGTPWTAKRFGHHNPIDYVKNWKTP
                     TLVIHGQRDFRVVYAQGLSTFTALQRRGIPSKLLIFPDENHWVLKPANSLQWHDTVLG
                     WLDEWLKK"
     gene            complement(464..2077)
                     /locus_tag="PLP50_07925"
     CDS             complement(464..2077)
                     /locus_tag="PLP50_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51515.1"
                     /translation="MSGALPRVLFRTKRGLCPSCGVPLELDEDAPEVTCRFCGAPAVL
                     ERRLRRVEPEVEGAPLRLYLDAASAEAGGDPSATPWVRSKAFRQSVVTRTLCPGCGEA
                     LEWRDDAERVDCPSCGTDCRLERRLQAPPLDPARGVPRRRTADERATADDDDPRTEQL
                     VWRVANEEDPRRRFALALRFEEWLFVNATAARLLPSLLRVLCEAEPALQVAIGDFVGK
                     LLCEGSVALRNATLRAIEPFAFERAVPPTLLFALGLGDGTSLKLLLDAAELAHRRGDD
                     DAATTALLAVNWIFQRTYPHHAVMGEILLHRLLYLSGPPLAFALLLARRRVTGTGFHY
                     SAETLLSFIDEAAVERPSLVPELARCFYVGLPKDEAEARSRMAFFGTLRTPAARSASL
                     REHLLPPEEAPDALYAELAALVLPLAADPGLGPAAEVALKRLVEIPRGVPAAVHDLVS
                     ERGRSLPAEMQRAYLRAVPDTPHLSWDDVGYGEVEREEPLAPELQSLLDRWNEDLRRA
                     ADEVDAARSSFRERKDELSGLDVPLFDQG"
     gene            complement(2094..3170)
                     /locus_tag="PLP50_07930"
     CDS             complement(2094..3170)
                     /locus_tag="PLP50_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPA51516.1"
                     /translation="MPDLADILELVDATGDLLAARAPADGPAVIRWGSQLVVREGQSA
                     LFLRDGRAMQLFEPGRHVLTTQNVAGLTGFVAGLAYGGETPFRAEVVFVGRQLFRDLR
                     WGTPEPVYIPDPVLLQVPVRANGRFAIRVADPALFVPKVVGTRPDFRQRDLEEFLRAQ
                     YVVSALTDALASLGKPFVELPRFTRELGTGVRAILAPEFAALGLELTDLSVNSVTTTE
                     EIQATLNGNAKIASEAFAKAKGTQYDLQARAAGAEALKRAGTSYREVGATDALKEVAS
                     NVGEGGGGSSLDAGVSLGAAMLVPQMMQGMLRPEAAPGGPAAHAAEEVDPVARLKQLK
                     ELLDLGAITQEEYDAKKAEWLKRL"
     gene            complement(3170..3658)
                     /locus_tag="PLP50_07935"
     CDS             complement(3170..3658)
                     /locus_tag="PLP50_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51517.1"
                     /translation="MSGSARALLERLGSLVPGYAGYADRETRRECDQALRLAVASRLG
                     AARATLDRRTADCARTGRLDLLEPLDSISRRVAALADALRHAPAGYAALFDAATVDAA
                     QLDRIYAFDLEMKEAGERVFDVAERVRAGVDAEALDALNAAVAAAEESLRRRGEVLRE
                     VR"
     gene            3962..5506
                     /locus_tag="PLP50_07940"
     CDS             3962..5506
                     /locus_tag="PLP50_07940"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPA51518.1"
                     /translation="MTGLVVLGAVLLVLVAVAVHDRLQKTHTIVSNFPVLGRFRYWFE
                     ALGGPLRQYIVTGNDEERPFSRDQRRWIYASAKKENSYFGFGTDNDLEKETGYVVVRH
                     STFPLHSPVPGEEGYDPGHPLPAAKVLGGARGRRHAFRPTSLVNTSAMSYGSLSHAAV
                     EAINRGVALAGAMQNTGEGGISDFHRKGGDIVWQIGTGYFGCRDENGRFSMERFLENV
                     ASAKVRAVEIKLSQGAKPGLGGVLPAAKVTPEISRIRHVPVGKDCISPSAHSAFSDVS
                     SLLDFVERLADATGLPVGIKSAVGDLGFFEELARQIETSGRAPDFVTIDGGEGGTGAA
                     PLVFTDHVAMPFKLGFASVYRAFAERGVAERVVFVGSGRLGFPEQALLALALGCDMVN
                     VAREAMLSIGCIQAQVCHTGRCPTGVATQDPWLVGGLDPTLKSARLANYLATLRKYLL
                     ALAHACGEVHPALVPLERIDILDGLSSRPAREVFGYQPGWGLPTEEERETIRRLMTGG
                     SASQAA"
     gene            complement(5525..6094)
                     /locus_tag="PLP50_07945"
     CDS             complement(5525..6094)
                     /locus_tag="PLP50_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA51519.1"
                     /translation="MTRPDPVVALAEEYPRFLAFLAKRTGDRAVAEEILQEAFAKAVT
                     KTHGLREDSSAVAWFYQVLRNALVDHHRRTASEGRGLAAYAAEQGGEARDEELEREVC
                     RCVGRIAADLKPEYAEALRVVEVDGRPLKELAERAGITPNNAAVRVHRAREALRKRLV
                     ATCSTCAAKGCTDCTCRRDEGNEGHEGRG"
     gene            6336..8453
                     /locus_tag="PLP50_07950"
                     /pseudo
     CDS             6336..8453
                     /locus_tag="PLP50_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
     gene            complement(8494..9621)
                     /locus_tag="PLP50_07955"
     CDS             complement(8494..9621)
                     /locus_tag="PLP50_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA51520.1"
                     /translation="MPAAPLESFFLWGPRQTGKSTLLRSLYPEAVWLDLLKTDEQIRY
                     ASRPALLREELEAVPPGLLVVIDEVQKAPGLLDEVHWLVENRGRVFALSGSSARKVRR
                     SHANLLGGRAVRFEMFGLTSEEVGPTFDLVRALNHGFLPRHYLSEAPGRLLRSYVTDY
                     LKEEVLAEGLSRNLPAFTTFLAAAALSDGELVSFATIARECGVSAPAVKGWFEVLVDT
                     LLGDFLPAFTKRPKRRVIGAPKFYFADVGVVNHLAKRGRMEPGSALFGKAFESWVRHE
                     LRAYSSYRERFFDLSYWRLASGIEVDFVVDDMVAAFEAKATPRVKSDDLNGLRQIRID
                     HPKLKERFVVSLEPKARVTEDGIVVLPWRVFVERLWGDELI"
     gene            complement(9739..10578)
                     /locus_tag="PLP50_07960"
     CDS             complement(9739..10578)
                     /locus_tag="PLP50_07960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51521.1"
                     /translation="MTLACDVSLAARIERAEARLLADAVARVGLRVGERGDVLSRPLA
                     GGIAAFAEPGSPLNKVAGLGFDGALPETELEEVEKGFARRECPVRVELSSLADPALGA
                     LLTRRGYVLEGFEDVLGLELPARDLPPIPEDVQVRPSGPEELELWLDVTVAGFLSPDT
                     QGVPSNESFEEEVLRRVIRDFAQAEGMTRYLAERGGVPAGGGSVRVADGLAQLCGAAT
                     LPAHRRRGIQTALLAERLAAAGRAGCDLAVVTTQPGSKSQENARRRGFALLYTRAVLV
                     RGG"
     gene            complement(10631..13237)
                     /locus_tag="PLP50_07965"
     CDS             complement(10631..13237)
                     /locus_tag="PLP50_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPA51522.1"
                     /translation="MTIPEKPPVTPLADRVPPRGTTDGDEILGLFLDWVADTGLTLYP
                     AQEEALLEIMAGKHVILGTPTGSGKSLVALGLHFKALCERRVSYYTSPIKALASEKFF
                     ALCGELGAENVGMRTGDASINAEGLVVCCTAEVLSNMALRMGEELDAPYVVMDEFHYY
                     ADRERGVAWQVPLLVLPGTQFLLMSATLGDTSAIAERLTHDTGREVAFVTSEERPVPL
                     DFAYAETPLHQTIEKLLEEGKSPVYVVNFTQRECAELAQALTSVRIGTKEEREAIREV
                     VAGRRLVTPYGKEFRRFLSFGVGVHHAGLLPRYRLLVEQLAQRGLLHVICGTDTLGVG
                     VNIPIRTVLFTKLAKFDGAKVGLLTVREFKQIAGRAGRRGFDTQGSVVAQAPEHIVEK
                     KQATKKSDASGKKVTKTFRGPAKGEISWTEETFQRLITRPPETLKSRFHLTHGMVLDL
                     IQRDADTNRDAPPPDRNFSALRDLVARCHEEDATKARLLKHAALLVRSLYRAGIVGMS
                     VEETGFRRVVVADDLQWDFSLHQALSIYAVTALERLDPASPTYALDVVTVVESILENP
                     DIVLRKQTDRAKGKLVEELKAEGVPYEERMAKLEEVTYPQPLADFLFGAFEAFREAHP
                     WAGGTEPKPKSIGREMFETFAGFSDYVKSYGLERSEGVLLRYLSQLYRTLDQTVPEPA
                     KTDELWDVLGFFRALVGETDASLLEEWESLRHPEELLEASAEPEKPKQAAWLRELLSD
                     PRVFAARVRSEMHLLVRALSRRDWEDAAERVWQPAENAEGAAEAAWTPERFEAALAPF
                     FEEHGDLVSGPESRLHQWTNLRPTGTRQWAVTQTLLDPEEENAWAVIADIDLRDETAP
                     DGPILRVTRIGT"
     gene            complement(13287..16271)
                     /locus_tag="PLP50_07970"
     CDS             complement(13287..16271)
                     /locus_tag="PLP50_07970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA51523.1"
                     /translation="MGRGTVPACVALLGALVLAPRASALDAGRPLSQAQVDVWRTENG
                     LPQDTVTSILRTREGHLWFGTYDGLVRFDGARFTVFDGKTTPLLATGSAFALMEDRHG
                     AIWVGRSENVLKYENGAFTQVLGADELGQGTVWALAEGADGTVWAGAGRGLVRFTAGK
                     ATLLTKENGLPPARLRTVCVDREGAVWIGTSGAGLARLRDGRVTRWTMTDGFPSDHVV
                     TVIADPSGGVWVATAGAGLVRIVGDSRRVWTPSDGLPTDQLTALALDAAGSLWIGTWG
                     SGVCRFRDGAFSVLAAPPLSNDKIWSILPDEEGFVWVGTWVGGLNRLRDRSFPSYGEP
                     EGLSNDNVRSVLHARDGSVWIATAGGGLNRFREGRFEVFRKADGLPSDEVSALREDRS
                     GALWVGTYTSGLARLRGGRIEAFGKDDGLPGDDVRVILEDREGTLWVATTTGVATSRD
                     GRRFERVSTPEWVSLNAVVSMLEDRGGAIWYGTSGDGLIRRDASGFRVLTTRDGLVSD
                     RIAALHEDEDGVLWIGSAWSGINRLKDGRLTSIRPEAGLAEGRVQTLIPDRFGALWLT
                     GNKGFQRILKRELNEVADGLRGAVRPLSFGLADGLRSASFASGQQPAGSVGPDGKIWL
                     PSYRGVVVVDPARIPPPPPPPGARVEEVVVDGGLRVARRELQIEPGRRLLEVRYAATT
                     LRPPELVGFRFRLDGFDDDWHEVGTRRSAYYTALPPGRYRFRVQSRVGDGAWGQEAEP
                     LSLVLEPAIHQTAWFRALAVLLLLSAAALLVRQRTVRLKQRHAELERLVGERTEALRE
                     ANERLSDLSFSDALTGIANRRRLDETLDAEWKRAIRFGHPLSLVLADIDFFKRYNDAL
                     GHPAGDRCLVEVARVLQANARRAGDVAARFGGEEFALLLPATSGADATVVAERTRAAL
                     EELAMPHPDGATPVVTASFGVATFVPESDGDPERLLATADAALYRAKRDGRNRVVSAT
                     PRGGRSPETGRSGEHRLPKTTG"
     gene            16446..17897
                     /locus_tag="PLP50_07975"
     CDS             16446..17897
                     /locus_tag="PLP50_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPA51524.1"
                     /translation="MHEMEPLFFGRTFDDFLFRPQRSPVASRRAVDLSMPLVPGLDLG
                     LPIVGANMDTVVGEEMARALALEGAFGFLHRSSPIEAQAARVRYVKTRHSYVIEKPLV
                     LPRTATIAEVRRLIEKHRSSGILIEETAGSGLLAGILSHRDLPHAPDGSERRVEELMT
                     PLARLLTRPPTVTMEEAEKAMWERRVEKLPLVDDAGRIRGLVTMRDLHLFKKKPWSVK
                     DERGRLRVGAAIGAAGDYLERAAALAEAEVDVVLMDVAHAHSEILAKAVPAFRSRFPR
                     IPLVVGNVATAEGARFLVDLGAAAVKVGVGPGRGCRTRLETGAGVPQLQAIREAYLAT
                     EGKVPIIADGGIRNDKDVFLALACGASTVMLGSLLSGTDESPGIVVDDPATRQKAKLY
                     RGMTSPEAVVDGSTDAGVADALATPAEGQSVRVPYVGSVVDVLARIAGHLRSAVSYAG
                     ETTLADAHRKIAADPARYLVPLSAAARTESFER"
     gene            17909..18295
                     /locus_tag="PLP50_07980"
     CDS             17909..18295
                     /locus_tag="PLP50_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010455492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPA51525.1"
                     /translation="MAIHVVRLGAPRGRGEGLRLGTVRRPPRGVPKAEFASRDFYDVW
                     LPDLAPSEELVKAAQAAADERAWKAFERKYRAEMSTPHAARLLDLLAALSHRTSLAVG
                     CYCADEARCHRSLLRALLAERGAELA"
     gene            18374..19117
                     /locus_tag="PLP50_07985"
     CDS             18374..19117
                     /locus_tag="PLP50_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51526.1"
                     /translation="MTAEEHVCPRCGIRFDADEEGVFATCPACGSLLHLGDEAEIAHQ
                     RLVPVLDEAAVLGRLARWLRDREVLADPKEVATRLLWFPFWLLPGDALVPAAPLLASN
                     LGAFRAPAGDRVAFREGEAGPAEIVPASVPPAAIVDDEATRRAARLLHVPFREVRFRL
                     WGHDYTVWFDAATGQALDFDLPPTSERRLDVTYALLLLALFAAGVYGVRTLFGGTAAP
                     GWVGAAVLAGAAGVFLAVAFALIRLTESR"
     gene            19174..20100
                     /locus_tag="PLP50_07990"
     CDS             19174..20100
                     /locus_tag="PLP50_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51527.1"
                     /translation="MASSALLGCPSCDSPLAVLAEGFPVRESVRPALGGDEAVARTRA
                     FWEGRKVPRAFLAAGSVEEPILVFVPFHERVRTFVLRPREGPVFVDRPSFAPAVRIPG
                     VPLEKLDAASASGATAREALNASALQRRALVFDPTEEAPKTPAISGGTVVEEKAGLVW
                     LPVWLVRCRHARNLYEAVLDGTSGSVLHARAPMERTTLLPEAVFFTYVFAFVFGMPLA
                     WWGKLVAFYFQLHWLGGLVLTVGTPGVVFWVLAFAWDRVRFRYEWTGDRLGVRREPVN
                     RPGRTLPEKIRDGFFTAARWLNGSPDKEEDRS"
     gene            20097..20630
                     /locus_tag="PLP50_07995"
     CDS             20097..20630
                     /locus_tag="PLP50_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51528.1"
                     /translation="MSGRPRVVARRCPDCGSLLLATAGVRWMTCTACPSALDPFAVPA
                     ERLPTFRPAGAGADEARRLAFYVFESAATEAPAWIWIPAFRILSTDSRSDPGALLARK
                     GYRPELVAAPLGSALARTPDEARALAALRLGAKAEEADLRAARLVSLSCRVSMGNVAE
                     KVSGFVAPAGSLRPPPA"
     gene            complement(20638..22158)
                     /locus_tag="PLP50_08000"
     CDS             complement(20638..22158)
                     /locus_tag="PLP50_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA51529.1"
                     /translation="MKLRTPPELRAAPHLDPDLVPGPPTPRRALLVNPFYPKDPHASF
                     GKHVLTPTLALTSVAGATPPGWVVALWDENLLQGPPPHEPFPSVVGITVHLTFASRAY
                     ELARWYRERGALVVLGGLHVSSCPDEAALHADALAVGDGVPLWPRILADAERGALEPV
                     YRAAFTAPYADAPPPRRDLLPRQSFLTTASLLATRGCHNRCDFCYLSTKGVKMPWSVK
                     EPAQVVDEWLATGEPYAVFLDNNLGADPEHLLRLCRALAPLERIWSAAVSIDVTDDPR
                     LVREMALAGCTGVFVGFETLNDPNLADARKRTPPVADYARRVGILHDNGIQVNGSFVL
                     GFDHDGPDVFDRTVAWIEANRLECATFHILTPYPGTPLFRRMEAGGRLLHRDWALYDT
                     AHVVFRPARMTPEELAEGYASCYRRLFSAGSIWRRRPLDPRAVAPYLAMSLLYKRANP
                     LWTFLIRHRLVASAWRPLVEATRRRHLVFRRRLAMREVSAAPASRQRETLALLPGA"
     gene            complement(22136..22927)
                     /gene="uppS"
                     /locus_tag="PLP50_08005"
     CDS             complement(22136..22927)
                     /gene="uppS"
                     /locus_tag="PLP50_08005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPA51530.1"
                     /translation="MKTDGASGSGERSVTRGAAALPRHVAIIMDGNGRWATRRGLPRA
                     VGHRAGAKAVRRVVEAARRCGVGTLTLYAFSSDNWRRPAAEVEALMGLFERSLGEQAR
                     RCVENGIRLRVIGRRDRLDPALVAAIDAAEARTAGGAAMELRIAVDYSGRGAVLAAIP
                     LLPAGPAPTREALLAALGRAGQGGAPEVDLLVRTGGERRTSDFLLWESAYAELFFSDR
                     LWPDVGAPDLDEALAWYAGRERRFGGLPAPAPATNGDPHEASHAA"
     gene            complement(23005..23391)
                     /locus_tag="PLP50_08010"
     CDS             complement(23005..23391)
                     /locus_tag="PLP50_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPA51531.1"
                     /translation="MSADANEGRFAYEGLDRVLHEKARLGILTSLATRPEGLLFGDLK
                     RLCALTDGNLNRHLEVLQEAGLVEVWKRFEGRRPQTLVRLSAEGRRRFLDYLGELEKV
                     LKDARKACALEDSASGSPLKAGWNPA"
     gene            complement(23388..23990)
                     /locus_tag="PLP50_08015"
     CDS             complement(23388..23990)
                     /locus_tag="PLP50_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51532.1"
                     /translation="MDVPQALDRIEEIHAQIAKGEVFRGYRPVPVAIAGVVGLLAGWL
                     QPRLVPEGDAARFLGYWIAVALGNVAINAAVIVAGYARCGDAFARRHTRRVVGQFVPC
                     LLAGAAVTLGLPAAGSALVRLLPGLWALLFGLGVFASRPYLARATGWVALYFVSAGTG
                     LLLFPPATLAALGLAHGAVFGAGLLGAAVVLHLDIPRGER"
     gene            complement(24022..24780)
                     /locus_tag="PLP50_08020"
     CDS             complement(24022..24780)
                     /locus_tag="PLP50_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPA51533.1"
                     /translation="MPSAQRPRRPAPSATDPLEVGLSAAGFALELFDALPDVVFFAKD
                     AEGRYRAVNDTLVRRLGRRTKRELLGKTARELFPAPLGERYLAQDLHVCRTGRLVEDL
                     LELHLYPDGHEGWCVTTKVPIRGPDGVVAGLAGTSRDVRSPEPGAGFEGLAGVLRLLH
                     ERPGGPHRVEELARLAELSPSRFSRRVRALFGLSASQLVIKVRVDAARRMLRESAAPI
                     ARVALACGYCDQSAFTRQFKAVTGLTPVQYRESP"
     gene            24851..25777
                     /locus_tag="PLP50_08025"
     CDS             24851..25777
                     /locus_tag="PLP50_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPA51534.1"
                     /translation="MAGAPPGTVGTIFNVQRGSFHDGPGIRTTVFLKGCPLRCPWCHN
                     PEGIAFAPEVLWSAGRCLSCGSCAAACPRNAGPLPAGAPLGSDGCSACRGCVEACPSA
                     AREVAGREVTVAEVVAEVLRDRAVYEESGGGVTFSGGEPLAQPAFLLSALAACRREGL
                     HVVVDTCGFAAREVALAAADLADLLLWDVKTLDPKRHLKLTGVWLAPILENLAAVSRA
                     GARIWLRLPVIPGVNDDDASLARAAALGERTAGVERVSLLPYHTTASGKRERLARAHA
                     SALAAAVSPSPERMASLTALFAPTGIPVTIGG"
     gene            25782..>27066
                     /locus_tag="PLP50_08030"
     CDS             25782..>27066
                     /locus_tag="PLP50_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPA51535.1"
                     /translation="MNPRTARLRQESLDAIPTISAERALLLTEYYRENLGRHSTPLLR
                     ARAFHDLCARKTLFLGEGELIVGERGPRPKAVPTYPELTCHSVEDLRILDSRPMTWYS
                     VPPEVIAAYEETVIPFWRGRSMRDQIFPALPPEWHAAYSAGVFTEFMEQRAPGHTVLD
                     DKVYRKGMNAFRREIAAALAALDFARDPEAVDKREELLAMDVACESLVLFAERHAALA
                     DERAALETDEMRRAELRKIADVCRRVPAEPPRDFHEALQMYWFCHLGVVTELNGWDAF
                     SPGHLDQHLLPFYRQGLANGTLTPESARELVECFFVKFNNHPAPPKVGVTAAESGTYT
                     DFANINLAGLLADGSDGSNEVTHLLLDVIDEMHLLQPSSNVQVSRKTPETVLKHVLRV
                     VRKGYGFPSIFNADTVVEEQLRQGKSLEDARAGGCS"
BASE COUNT         3675 a         9958 c         9750 g         3683 t
ORIGIN      
        1 cgacgagcgg atggcctact acgacaccga ggagctctgg ttccccgagt gggagaacgg
       61 cgggacgccc tggacggcga agcgcttcgg gcaccacaac ccgatcgact acgtgaagaa
      121 ctggaagacc ccgacgctcg tcatccacgg ccagagggac ttccgagtcg tctacgcaca
      181 ggggctctcg acgttcaccg ccctccagcg ccgcggcatc ccgagcaagc tcctcatctt
      241 ccccgacgag aaccactggg tcctcaagcc cgcgaactcc ctccagtggc acgacaccgt
      301 gctcggctgg ctggacgagt ggctgaagaa gtgacggtgt gaggcgcggc gccacccctg
      361 gcgtcgcgcc acctgaaggt ccgagagcgc ccggcgtgag ccgggcgctc tcgtttccgg
      421 ggtgctcgac gaccgggcgg ggacgatcgt ggggaggccg ctctcagccc tggtcgaaga
      481 gcggaacgtc gagccccgag agctcgtcct tccgctcgcg gaaggacgaa cgcgccgcgt
      541 cgacctcgtc cgcggcgcgg cggaggtctt cgttccagcg gtcgaggagc gactgcagct
      601 ccggggcgag cggctcctcg cgctcgacct ctccgtatcc cacgtcgtcc cacgagaggt
      661 ggggcgtgtc gggcaccgcg cggagatagg ccctctgcat ctccgccgga aggctccggc
      721 cccgctcgga gacgaggtca tggaccgccg cggggactcc gcgcggaatc tcgacgagcc
      781 gcttcagcgc gacctcggcc gcggggccga gcccggggtc cgccgcgagc gggaggacga
      841 gggccgccag ctccgcgtag agggcgtccg gcgcctcctc aggcgggagg aggtgctcgc
      901 ggagcgaagc cgatcgcgcg gccggcgtgc gcagcgtccc gaagaacgcc atccgcgacc
      961 gggcctccgc ctcgtccttc gggaggccga cgtagaagca gcgcgcgagc tcggggacga
     1021 gggaggggcg ctcgacggcg gcctcgtcga tgaacgagag gagcgtctcg gcggagtagt
     1081 ggaagccggt gccggtgacg cgccggcgcg cgaggaggag cgcgaacgcg agcggcgggc
     1141 cgctcaggta gagcagacgg tggaggagga tctcccccat cacggcgtgg tgcgggtagg
     1201 tccgctggaa gatccagttc acggcgagga gagcggtcgt cgccgcgtcg tcgtcgccgc
     1261 gccggtgggc gagctcggcg gcgtcgagga ggagcttcag ggaggtcccg tcgccgaggc
     1321 cgagcgcgaa gaggagcgtc ggagggaccg cccgctcgaa ggcgaacggc tcgatcgccc
     1381 ggagcgtcgc gttgcgaagc gcgacgctcc cttcgcagag gagcttcccg acgaagtcgc
     1441 cgatcgccac ctggagggcc ggctccgcct cgcagaggac tcggaggagc gagggaagga
     1501 ggcgcgcggc ggtcgcgttc acgaagagcc actcctcgaa gcggagcgcg agcgcgaacc
     1561 ggcgcctcgg gtcctcctcg ttcgcgacgc gccagacgag ctgctccgtt cgcggatcgt
     1621 cgtcgtccgc cgtggcgcgt tcgtcggcgg tgcgccgccg cggaacgccg cgggccgggt
     1681 cgagaggcgg cgcctgtagc cgccgctcga ggcggcagtc ggtgccgcag gagggacagt
     1741 cgaccctctc ggcgtcgtcc ctccactcga gcgcctcgcc gcagccgggg cagagcgtcc
     1801 gcgtcacgac ggactgccgg aaggccttcg acctcaccca gggcgtcgcc gacgggtccc
     1861 cccccgcctc ggccgaggcg gcgtcgaggt agagccggag aggtgccccc tcgacctccg
     1921 gctcgacccg ccggaggcgc ctctcgagca cggccggcgc gccgcagaag cggcacgtca
     1981 cctcgggggc gtcctcgtcg agctcgagcg ggacgccgca cgacgggcag aggccgcgct
     2041 tcgtccggaa gaggacgcgc ggcagggcgc cgctcaacgg gcgcgtccgc cgctcagagc
     2101 cgcttcagcc actcggcctt cttcgcgtcg tactcctcct gcgtgatcgc gccgaggtcg
     2161 aggagctcct tgagctgctt caggcgcgcg acggggtcga cctcctccgc cgcgtgcgcg
     2221 gccgggccgc ccggcgcggc ctccggcctc agcatcccct gcatcatctg cggaacgagc
     2281 atcgcggcgc cgagcgagac tccggcatcg agagaggagc cgccgccccc ctcgccgacg
     2341 ttcgacgcga cctccttcag ggcgtcggtc gcgcccacct cgcggtagct cgtcccggcc
     2401 ctcttcagcg cctcggctcc ggcggcccgc gcctggaggt cgtactgggt cccctttgcc
     2461 ttcgcgaagg cctctgaggc gatcttcgcg ttcccgttca gcgtcgcctg gatctcctcg
     2521 gtcgtcgtca ccgagttgac ggaaaggtcc gtcagctcga ggccgagcgc ggcgaactcg
     2581 ggggcgagga tcgcgcgcac gccggtgccg agctcccgcg tgaaccgcgg cagctcgacg
     2641 aacggcttcc cgagcgaggc gagggcgtcg gtgagggccg acacgacgta ctgcgcgcgc
     2701 aggaactcct cgaggtcgcg ctggcggaag tccggccgcg tgccgacgac cttcgggacg
     2761 aagagggcgg ggtcggcgac gcggatcgcg aagcgtccgt tcgcgcgcac cgggacctgc
     2821 aggaggaccg ggtcggggat gtagaccggc tccggcgtcc cccaccgcag gtcgcggaag
     2881 agctgacgcc cgacgaagac gacctcggcg cggaacgggg tctccccgcc gtaggcgagg
     2941 ccggcgacga acccggtcag ccccgcgacg ttctgcgtcg tcaggacgtg ccgccccggc
     3001 tcgaagagct gcatcgcgcg tccgtcgcgg aggaagagcg cgctctggcc ttcgcgaacg
     3061 acgagctggg agccccagcg gatgacggcg ggcccgtcgg ccggggcccg cgcggcgagg
     3121 aggtcgcccg tcgcgtcgac gagctcgagg atgtcggcca ggtcgggcat cacctcacct
     3181 cccggaggac ttcgccgcgg cgacggaggg actcttccgc ggccgcgacg gccgcgttca
     3241 gagcatcgag agcctcggcg tcgacgccgg cccgaacgcg ctccgccacg tcgaacaccc
     3301 gctcgcccgc ctccttcatc tcgaggtcga acgcgtagat ccggtccagc tgcgccgcgt
     3361 cgaccgtcgc cgcgtcgaag agcgccgcgt agcccgcggg ggcgtgtcgc aacgcgtccg
     3421 ccagagccgc gacccggcgg gaaatcgaat cgagcggctc gagcaggtcg agccggcccg
     3481 tccgggcgca gtcggccgtc ctgcggtcga gggtggcgcg ggccgcgccg aggcgcgagg
     3541 cgacggcgag ccgcagcgcc tggtcgcact cccggcgcgt ctcccgatcc gcgtagcccg
     3601 cgtagcccgg cacgagggag ccgagcctct cgaggagcgc tctcgccgag ccgctcatcg
     3661 aggcctcttc gcgccgaggg ccggcaccgc gccggcgcgc tcccgcgccg gtcccacgcc
     3721 cccggggcgt cgtgcagaca gccgttgcct catcttccgg acctcctcgt gcgggtcgat
     3781 ggcgggaggg taattcagag cgcccctgtt cggcgaacgg acggcgcgcc ctgccgccga
     3841 ggcgtgtcgg ccgtcacccg gacgccgcgg accttcctct gcgggcccgt cgcggtgcta
     3901 gcctcgccgc ctcgagacaa ccgaggagaa gggcgcgccg gtggcgcgcg aggggggaac
     3961 catgacggga ctcgtcgttc tcggcgccgt gctgctcgtt ctcgtcgcag tcgcggtcca
     4021 cgaccgcctc cagaagacgc acacgatcgt ctcgaacttc ccggtcctcg gacgcttccg
     4081 ctactggttc gaggcgctcg gcgggcctct ccggcagtac atcgtcaccg ggaacgacga
     4141 agagcgcccc ttcagccgcg accagcgccg ctggatctac gcgtcggcga agaaggagaa
     4201 cagctacttc ggcttcggga ccgacaacga cctcgagaag gagacgggct acgtcgtcgt
     4261 ccgtcactcc acgttcccgc tccactcgcc cgtcccgggc gaggaggggt acgaccccgg
     4321 ccaccccctg cccgccgcga aggtcctcgg cggggcgcgc ggacgccgcc acgcgttccg
     4381 gccgacgagc ctcgtgaaca cgtcggcgat gagctacggc tccctgagcc acgccgcggt
     4441 cgaggcgatc aaccggggcg tggccctcgc gggggcgatg cagaacaccg gcgaaggggg
     4501 catctccgac ttccaccgga agggcgggga catcgtctgg cagatcggca ccggctactt
     4561 cggctgccgg gacgagaacg gccgcttctc gatggagcgc ttcctcgaga acgtcgcgag
     4621 cgcgaaggtg cgcgccgtgg agatcaagct ctcgcagggg gccaaaccgg gtctcggcgg
     4681 cgtccttccg gccgcgaagg tgacgcccga gatctcgcgg atccggcacg tgcccgtcgg
     4741 gaaggactgc atcagcccgt cggcccactc ggccttctcg gacgtctcgt ccctcctcga
     4801 cttcgtcgag cgcctcgcgg acgcgaccgg cctccccgtg gggatcaaga gcgccgtcgg
     4861 cgaccttggc ttcttcgagg agcttgcccg gcagatcgag acgagcgggc gcgcgcccga
     4921 cttcgtgacg atcgacggag gcgagggagg gaccggggcg gcgccgctcg tcttcaccga
     4981 ccacgtcgcg atgccgttca agctcggctt cgcgagcgtc taccgcgcct tcgccgagcg
     5041 cggcgtcgcc gagagggtcg tcttcgtcgg ctcggggagg ctcggcttcc ccgagcaggc
     5101 gctcctcgcg ctcgcgctcg gctgcgacat ggtgaacgtc gcgagggagg cgatgctctc
     5161 gatcggctgc atccaggccc aggtgtgcca cacgggccgc tgcccgaccg gtgtcgcgac
     5221 gcaggacccg tggctcgtcg ggggcctcga cccgaccctg aagtcggcac ggcttgcgaa
     5281 ctatctcgcg acgctccgca agtacctcct cgccctcgcc cacgcctgcg gcgaggtcca
     5341 cccggcgctc gtcccgctcg agaggatcga catcctggac ggcctctcct cgaggcccgc
     5401 gcgcgaggtg ttcggctacc agcccggctg gggcctcccg acggaggagg agcgggagac
     5461 gatccgccgg ctcatgacgg ggggctcggc gagccaggcc gcatagcgga aggccgcggc
     5521 ttcgtcagcc gcggccctcg tgtccctcgt tcccttcgtc gcgccggcag gtgcagtcgg
     5581 tgcacccctt cgccgcgcag gtcgagcagg tcgcgacgag cctcttccgg agcgcctcgc
     5641 gggcccggtg gacgcggacc gccgcgttgt tcggagtgat cccggcgcgc tccgcgagct
     5701 ccttcagcgg ccggccgtcg acctcgacga cgcggagcgc ctcggcgtac tcgggcttga
     5761 ggtcggcggc gatccgcccg acgcagcggc agacctccct ctcgagctcc tcgtcccgcg
     5821 cctcgccgcc ctgctccgcc gcgtacgccg ccagcccgcg gccctccgag gcggtccgcc
     5881 ggtggtggtc gacgagggcg ttgcggagga cctggtagaa ccaggcgacg gcggaggagt
     5941 cctcgcggag gccgtgcgtc ttcgtcacgg ccttcgcgaa ggcctcctgg aggatctcct
     6001 cggccacggc gcggtcgccc gtccgcttcg cgaggaacgc gaggaagcgc gggtactcct
     6061 ccgcgagggc gacgaccggg tcggggcggg tcacccgcgc attctgacaa ggtccgagcg
     6121 gccgctcccc ccggggtcgt ccgtccccgg gatgccgacg accgaggtgt aaggaagccc
     6181 accggtccgc gtctcccgtt cgagagagag gagatgccat ggaatcccac gctcacgccc
     6241 acccggacgg ttcggcttcg ccggaccgtc gttccagctg tcactccccg acgacgccaa
     6301 agcctccttc ggcctcttcg cccctagccc cagcttcccg ctgggtctgc ccaatgcacc
     6361 ccgaggtcgt ctccgacggc ccgggcgact gcccggtctg cggcatggcg ctcgagcccg
     6421 agatgccgac cgcggaggtc gagtctcccg agctcgccga catgacgcgg aggctgaagg
     6481 tggcgaccgc gctcggcctg ccggtcctcg tcctcgcgat gtcggacatg gtcccgggcc
     6541 agccgctcca gcacgccgtc tcgtccggac tcctcacgct cgcccaggcc ctcctcgcga
     6601 cgccggtcgt cgcctgggcc ggggcgccgt tcttccggcg cgcctggacc tccgtcgtga
     6661 accgcagccc gaacatgttc acgctcatcg gcctcggcgt cgcggccgcc tggctctaca
     6721 gcgccgtggc cctcgtcgtc gccgctctcc gccccgacct cgttcccgag gtctaccggg
     6781 ggcacggcgg cgcgcccctc gtctacttcg aggcggcggc cgtcatcacg gcgctcgccc
     6841 tcctcggcca ggtcctcgag ctgaaagccc ggagccggac gagctcggcc atccgcgccc
     6901 tcctcgacct ctcgccgaag acggcgcgga ggatcgaagg agaaggccgc gaggtggacg
     6961 tccccgtcga gcacgtcgcc gcgggcgacc gcctccgcgt gcggcccggc gagcgcgtcc
     7021 cggtggacgg gacgatccag gacgggaact cctgggtcga cgagtcgatg atcacgggcg
     7081 agcccatccc ggtcgagaag acgccgggga gcgccgtcac cggcgggacc gtgaacggcg
     7141 ccggcggctt cgtgatggtc gccgagcggg tcggcgaggg gacgacgctc gcgcggatcg
     7201 tgaagctcgt cgccgaggcc cagaggagcc gcgccgcgat ccagcagctc gccgaccgcg
     7261 tctcggcctg gttcgtcccg gcggtcgtgg tcgcggccct cgcgaccttc gtgctctgga
     7321 tggccctcgg acccgagccc cgattcgtcc acgccctcgc ctcggcgatc gccgtcgtca
     7381 tcatcgcctg cccgtgcgcc ctcggcctcg cgactcccct ctcgatcatg gtcgcgaccg
     7441 gacgcggcgc gctctccggc gtcctcgtga agaacgccga cgccctcgag cggctcgccg
     7501 ccgtggacgt cctcgtcgtc gacaagaccg gcacgctgac cgaagggcgg cccggcgtcg
     7561 cggcgctcga agccgtcgag ccgttcacga aggacgaggt cgtccgcctc gccgccgccc
     7621 tcgagcgcgc aagcgagcac ccgctcggcg cggccatcgt ggccgccgcg ggcgccggcc
     7681 cgcttcccct cgtctcggag ttccggtccg tcacgggccg cggcgtcgcg ggccgcgtct
     7741 cgggccacga cgtcctcgtc gggagccttc gcttcctggc ggaggcgggg gtcgagacga
     7801 cggcgctcgc cgccctcgcg acggcccacc aggaagaagg acggacggcc gtcaccgtcg
     7861 ccgtcgacgg ccgccccgcg ggcctcgtcg ccgtcgccga cccggtcaag gcgtcggccc
     7921 cggccgccct cgcggccctc cgcaccgacg gcatcgagat cgtcctcctg accggcgatg
     7981 cgcgccgctc ggccgccgcc gtggcgagat cactcggcat cacaagcttc gaggccgagg
     8041 tcctccccga ggagaagggg gccgtcgtcg agcggctccg tgcggaaggg cgggtcgtcg
     8101 cgatggccgg agacggcgtg aacgacgccc cggccctcgc ccgggccgac gtcggcatcg
     8161 cgatggggac cgggaccgac gtcgcgatcg agagcgccgc cgtcacgctc ctccacggcg
     8221 acctcgcggg cctcgtccgc gcgcggcgcc tcggccgcgc cacgatgcgg aacatccgcc
     8281 agaacctcgc ctgggcgttc ggctacaacc tcctcggcgt ccctctcgcc gccgggctcc
     8341 tctaccccgt cttcggcgtc ctcctctcgc cgatggtcgc cggcgccgcc atgagcctca
     8401 gctcggtctc ggtcatcgtg aactcgctcc ggctgcgcag gctcgacctc tgaatcgaca
     8461 cgggaggggg cgacggtcgt ggggcgggac cggtcagatc agctcgtcgc cccagagccg
     8521 ctccacgaac acgcgccagg gaaggaccac gatcccgtcc tcggtgacgc gggccttcgg
     8581 ctcgagcgag acgacgaagc gctccttcag cttcggatgg tcgatccgga tctgccgaag
     8641 cccgttcagg tcgtccgact tcacgcgggg cgtcgccttc gcctcgaagg cggcgaccat
     8701 gtcgtcgacg acgaagtcga cctcgatgcc gctcgcgagc ctccagtacg acaggtcgaa
     8761 gaaccgctcc ctgtagctcg agtacgcgcg gagctcgtgc cgcacccagc tctcgaaggc
     8821 cttcccgaag agagcggacc ccggctccat ccgccctcgc ttcgcgaggt ggttcacgac
     8881 tccgacgtcg gcaaagtaga acttcggcgc gccgatgacc cgtcgcttcg gccgcttcgt
     8941 gaaggccggg aggaagtcac cgaggagcgt gtcgacgagg acctcgaacc aacccttcac
     9001 ggccggggcc gagacgccgc actcgcgcgc gatcgtcgcg aagctgacga gctcgccgtc
     9061 cgagagcgcc gccgccgcca ggaacgtcgt gaaggccggg agatttcgcg aaaggccctc
     9121 ggcgaggact tcctccttga ggtagtcggt cacgtacgac cggaggagcc tacccggcgc
     9181 ctcggagagg tagtgcctcg gcaggaaccc gtggttcagc gcccggacga ggtcgaaggt
     9241 cggcccgacc tcctccgacg tcagcccgaa catctcgaag cggacggccc tcccgccgag
     9301 gaggttcgcg tgcgaccggc gcaccttgcg cgcgctcgag cccgagaggg cgaatacccg
     9361 cccccggttc tcgacgagcc agtgaacctc gtcgaggaga ccgggggcct tctgcacctc
     9421 gtcgatgacc acgagcaggc ccggcgggac ggcctcgagc tcctcccgga gaagcgcggg
     9481 tctcgacgcg taccggatct gctcgtcggt cttcaggagg tcgagccaga cggcttcggg
     9541 atagagcgac ctcaggagag tgctctttcc cgtctggcgg ggcccccaga ggaagaacga
     9601 ctcgaggggg gcggcgggca aggggacgtt tctacgaaac ataactttcg attgtcatga
     9661 taatcgaaag taatacgtcc gaagcggaga ccgctccggg caacggcgta gcgggcccgg
     9721 gaccggcacc ggagccgatc agcccccgcg gacgaggacc gcgcgggtgt agaggagcgc
     9781 gaagccgcgc cgccgggcgt tctcctgcga cttcgagccg ggctgcgtcg tgacgacggc
     9841 gaggtcgcag cccgcgcggc ccgcggccgc gagccgctcc gcgaggagcg ccgtctggat
     9901 gccacggcgc cggtgcgcgg ggagcgtggc ggcgccgcag agctgggcga ggccgtcggc
     9961 gacgcgcacg ctccccccgc cggcgggaac gccgccccgc tccgcgagat accgcgtcat
    10021 cccctcggcc tgcgcgaagt cgcggatgac ccgccggagg acctcctcct cgaacgactc
    10081 gttcgagggg accccctgcg tgtccggcga gaggaagccg gcgacggtga cgtcgagcca
    10141 gagctcgagc tcttcgggtc cggacggacg gacctgcacg tcctcgggaa tcggcgggag
    10201 gtcccgcgcc gggagctcga ggccgaggac gtcctcgaac ccctcgagga cgtagccgcg
    10261 ccgcgtgagg agggcgccga gcgccgggtc cgcgaggctc gagagctcca cgcgaaccgg
    10321 gcactcccgg cgcgcaaagc ccttctccac ctcttcgagc tccgtctcgg ggagcgcgcc
    10381 gtcgaacccg aggccggcga ccttgttcag cggcgagccg ggctccgcga acgccgcgat
    10441 ccctccggcc agggggcgcg aaaggacgtc tccccgctcg ccgacgcgga gaccgacccg
    10501 ggcgacggcg tcggcgagga ggcgggcttc ggcccgttcg atccgggcgg ccagggaaac
    10561 gtcgcaggcg agcgtcatgc ggccccgatg atatcggcgg caatcgtctg cgccgaagga
    10621 ggtcgcggcg ctacgtcccg atccgcgtca cccggaggat cgggccgtcg ggggccgtct
    10681 cgtccctgag gtcgatgtcg gcgatgacgg cccaggcgtt ctcctcctcc gggtcgagga
    10741 gcgtctgcgt gaccgcccac tgccgagtgc cggtcggtcg caggttcgtc cactggtgga
    10801 ggcgcgactc cgggccggaa acgagatcgc cgtgctcctc gaagaacggc gcgagggccg
    10861 cctcgaagcg ttcgggcgtc caggccgcct ccgccgcgcc ttcggcattc tccgccggct
    10921 gccagacccg ctcggcggcg tcctcccagt cccgccgcga gagggcgcgg acgaggaggt
    10981 gcatctcgga gcggacgcgc gccgcgaaga cgcgcgggtc ggacagcagc tcgcggagcc
    11041 acgcggcctg cttcggcttc tccggctcgg cgctcgcctc gaggagctcc tcggggtgcc
    11101 gcaggctctc ccactcctcg aggaggctcg cgtccgtctc gccgacgagc gcgcggaaga
    11161 agccgaggac gtcccagagc tcgtccgtct tcgccggctc ggggacggtc tggtcgagcg
    11221 tccggtagag ctgcgagagg tagcggagga ggaccccctc gctccgctcg aggccgtagc
    11281 tcttcacgta gtccgagaag cccgcgaacg tctcgaacat ctcgcgcccg atcgacttcg
    11341 gcttcggctc ggtccccccg gcccacgggt gcgcctcgcg gaacgcctcg aaggcgccga
    11401 agaggaagtc ggcgaggggc tgcgggtacg tgacctcctc cagcttcgcc atccgctcct
    11461 cgtacggcac cccctcggcc ttcagctcct cgacgagctt ccccttcgcc cggtcggtct
    11521 gcttccggag gacgatgtcg gggttctcga ggatcgactc gacgacggtg acgacgtcga
    11581 gggcgtacgt gggcgaggcg gggtcgagcc gctcgagggc cgtgacggca tagatcgaga
    11641 gcgcctggtg gagggagaag tcccactgga ggtcgtcggc gacgacgacg cgccggaagc
    11701 cggtctcctc gaccgacatc ccgacgatcc ccgcccggta gagcgagcgg acgaggagcg
    11761 ccgcgtgctt gagcagccgc gccttcgtcg cgtcctcctc gtgacagcgg gcgacgaggt
    11821 cgcggagcgc cgagaagttc cgatcgggcg gcggggcgtc gcggttcgtg tcggcgtcgc
    11881 gctggatcag gtcgaggacc atcccgtgcg tgaggtggaa gcgcgacttc agcgtctcgg
    11941 gcggccgggt gatgaggcgc tggaaggtct cctcggtcca ggagatctcc cccttcgccg
    12001 gcccccggaa cgtcttcgtg accttcttcc cggaggcgtc ggacttcttc gtcgcctgct
    12061 tcttctcgac gatgtgctcg ggcgcctgcg cgacgacgct cccctgcgtg tcgaagccgc
    12121 gccggccggc gcgcccggcg atctgcttga actcgcggac ggtgaggagg ccgaccttcg
    12181 ccccgtcgaa cttcgcgagc ttcgtgaaga ggaccgtccg gatcgggatg ttcacgccga
    12241 cgccgagcgt gtcggtcccg cagatgacgt ggaggagccc tctctgggcg agctgctcga
    12301 cgaggaggcg gtagcgcggc aggagcccgg cgtggtggac gccgacgccg aaggagagga
    12361 agcgccggaa ctccttgccg tagggggtga cgagccgccg gccggcgacg acctcgcgga
    12421 tcgcctcgcg ctcttccttc gtgccgatcc tcacgctcgt cagcgcctgg gccagctcgg
    12481 cgcactcgcg ctgcgtgaag ttgacgacgt agaccgggct cttcccctct tccaggagct
    12541 tctcgatcgt ctggtggagc ggcgtctcgg cgtacgcgaa gtcgagcggg acggggcgct
    12601 cctcggacgt cacgaacgcg acctcgcgcc cggtgtcgtg cgtcagccgc tcggcgatcg
    12661 ccgacgtgtc gccgagcgtc gccgacatga ggaggaactg cgtccccggc aggacgagga
    12721 gcggcacctg ccaggcgacg ccccgttccc tgtcggcgta gtagtggaac tcgtccatga
    12781 cgacgtacgg cgcgtcgagc tcctcgccca tccggagcgc catgttcgag aggacctccg
    12841 ccgtgcagca gacgacgagg ccttcggcgt tgatgctcgc gtctcccgtc cgcatcccga
    12901 cgttctccgc tcccagctcg ccgcagagcg cgaagaactt ctcgctcgcg agcgccttga
    12961 tcgggctcgt gtagtacgag acgcgccgct cgcagagcgc cttgaagtgg agcccgaggg
    13021 cgacgaggga cttccccgac ccggtcggcg tcccgaggat gacgtgcttg cccgccatga
    13081 tctcgaggag cgcctcctcc tgggcgggat agagcgtcag cccggtgtcg gcgacccagt
    13141 cgaggaagag gccgaggatc tcgtcgccgt cggtcgtgcc gcggggcggg acgcggtcgg
    13201 cgaggggggt gacgggcggc ttctcaggga tcgtcatggc gggaggacgg agtttcccat
    13261 cctctcgccc ggggacgaaa cgacgctcag ccagtcgtct tcgggagccg gtgctcgccc
    13321 gaccgccccg tctccggaga acgtccgccg cgcggcgtgg cgctgacgac gcggttccga
    13381 ccgtccctct tggcccgata cagggcggcg tccgccgtcg cgaggaggcg ctccgggtct
    13441 ccgtcggact ccggcacgaa ggtcgcgacg ccgaagctcg cggtcacgac cggggtggct
    13501 ccgtcggggt gcggcatcgc gagctcctcg agggccgccc gggtccgctc ggccacgacc
    13561 gtggcgtccg ctccgctcgt cgccgggaga agcagcgcga actcctcgcc gccgaagcgg
    13621 gccgcgacgt cgcccgcccg gcgggcattc gcctgcagga cgcgcgcgac ctccacgagg
    13681 cagcggtccc cggccgggtg cccgagcgcg tcgttgtacc gcttgaagaa gtcgatgtcc
    13741 gcgaggacga gcgagagcgg gtgcccgaag cggatcgccc gcttccactc cgcgtcgagc
    13801 gtctcgtcca gccggcggcg gttcgcgatg cccgtgagcg cgtcggagaa ggagaggtcg
    13861 gagagccgct cgttggcctc tcgcagcgcc tccgttcgct ctccgacgag cctctcgagc
    13921 tcggcgtggc gctgcttcag ccggacggtc ctctgccgga ccaggagagc ggccgccgag
    13981 aggagaagga gcacggcgag ggcccggaac cacgcggtct ggtggatcgc cggctcgagg
    14041 acgagcgaga gaggctccgc ctcctgcccc caggcgccgt cgccgacccg gctctggacc
    14101 cggaagcgat accgcccggg cggcagggcg gtgtagtagg cggaccgccg cgtccccacc
    14161 tcgtgccagt cgtcgtcgaa gccgtcgaga cggaagcgga acccgacgag ctcggggggc
    14221 cgcagcgtcg tggccgcata gcggacctcg aggagccttc tccccggctc gatctgcagc
    14281 tcccgcctcg cgacgcggag gcctccgtcg acgacgacct cttcgacccg cgctccggga
    14341 ggaggcggag gcggcgggat ccgggcgggg tcgacgacga cgacgccgcg ataggacggg
    14401 agccagatct tcccgtcggg accgaccgac ccggcgggct gctgcccgct cgcgaacgag
    14461 gcgctccgga ggccgtcggc caggccgaac gacagcggcc gcacggcgcc gcgcaggccg
    14521 tccgcgacct cgttcagctc ccttttcagg atccgctgga accccttgtt cccggtcagc
    14581 cagagcgcgc cgaagcgatc cgggatgagc gtctgcaccc ggccctccgc gagcccggct
    14641 tcgggacgaa tcgaggtgag gcggccgtcc ttcaggcggt tgatgccgct ccaggcgctc
    14701 ccgatccaga ggaccccgtc ctcgtcctcg tgcagcgccg cgatccggtc gctcacgagg
    14761 ccgtctcgcg tcgtcaggac gcggaagccc gaggcgtccc gccggatgag gccgtcgccg
    14821 gacgtgccgt accaaatcgc ccccccgcgg tcctcgagca tgctgacgac cgcgttgagc
    14881 gagacccact ccggcgtcga gacgcgctcg aatcggcggc catcgcgcga cgtggcaacg
    14941 cccgtcgtcg tggcgaccca gagcgtcccc tcacggtcct cgaggatgac gcggacgtcg
    15001 tcgccgggca ggccgtcgtc tttcccgaac gcctcgatcc gtccgccccg cagccgcgcg
    15061 agacccgagg tgtaggtgcc gacccagagc gctccggagc ggtcttcccg gagcgccgac
    15121 acctcgtcgc tcggcaggcc gtccgccttc cggaagacct cgaagcgccc ctcgcggaac
    15181 cggttgagcc ctccaccggc ggtggcgatc cagacgctcc cgtcgcgcgc gtgaaggacg
    15241 gaccggacgt tgtcgttcga gagcccctcg ggctcgccgt aggacgggaa gctccggtcg
    15301 cggagccggt tcagcccgcc gacccaggtc ccgacccaga cgaatccctc ctcgtccggg
    15361 aggatcgacc agatcttgtc gttcgagagc ggcggcgcgg cgaggacgga gaacgccccg
    15421 tcgcgaaacc ggcagacgcc gctcccccag gtcccgatcc agagcgatcc tgccgcgtcg
    15481 agcgcgagcg ccgtgagctg gtcggtcggc aggccgtcgg agggggtcca gacgcgccgg
    15541 ctgtcgccga cgatccggac gaggccggcg ccggccgtcg cgacccagac cccgcccgac
    15601 gggtcggcaa tgaccgtcac gacgtggtcg ctcgggaagc cgtccgtcat cgtccagcgc
    15661 gtcacgcggc cgtcccgcag ccgcgcgagg ccggcgccgc tggtgccgat ccagaccgcc
    15721 ccttcccggt cgacgcagac cgtccgaagc cgcgccggag gcaggccgtt ctccttcgtc
    15781 aggagcgtcg ccttgccggc cgtgaagcga acgagcccgc gacccgcgcc ggcccagacg
    15841 gtgccgtccg cgccctcggc gagcgcccag accgtcccct gccccagctc gtcggcgccg
    15901 aggacctgcg tgaaggcgcc gttctcgtac ttcaggacgt tctcgctccg cccgacccag
    15961 atcgcgccgt ggcggtcctc catcagcgcg aaggctgagc ccgtcgcgag gagcggcgtc
    16021 gtcttcccgt cgaaaaccgt gaaccgggcg ccgtcgaaac ggacgagccc gtcgtacgtg
    16081 ccgaaccaga ggtgcccttc gcgcgtccgg aggatcgacg tgacggtgtc ctgggggagg
    16141 ccgttctccg tccgccagac gtcgacctgg gcctgcgaga gggggcgtcc cgcgtcgagg
    16201 gccgacgcgc gcggggcgag gacgagcgcg cccaggagag cgacgcacgc ggggaccgtg
    16261 ccccggccca ctctccacac cggtccccgc ggggtctcag ctgctgccag aaccctctcc
    16321 tcgtccgtcc cggcgccgag gaaggtctcc cggctgccgc gacggtccat tctagcggtc
    16381 tgcccgtccg ccgccaggcg aggacgacgt atccacaccc cgacggaggt ggaagaatgg
    16441 ggacgatgca cgagatggag cccctcttct tcggccgcac gttcgacgac ttcctctttc
    16501 gcccgcagcg gagccccgtc gcctcccgcc gcgccgtgga cctctcgatg ccgctcgtcc
    16561 ccggactcga cctcggcctc ccgatcgtcg gggcgaacat ggacacggtc gtcggcgagg
    16621 agatggcccg ggcgctggcc ctcgagggcg ccttcggctt cctccaccga agctccccga
    16681 tcgaggcgca ggcggcgcgc gtccgctacg tgaagacgcg ccactcctac gtgatcgaga
    16741 agccgctcgt cctccctcgg accgcgacga tcgccgaggt ccgccggctc atcgagaagc
    16801 accgctcgag cggcatcctg atcgaggaga ccgccggctc gggcctcctc gccgggatcc
    16861 tctcccaccg cgaccttccg cacgccccgg acgggagcga gaggcgggtc gaggagctga
    16921 tgaccccgct ggcgcggctc ctgacgcgcc cgccgaccgt caccatggag gaggccgaga
    16981 aggcgatgtg ggagcgccgc gtcgagaagc tcccgctcgt cgacgacgcc ggacggatcc
    17041 gtggcctcgt cacgatgcga gacctccacc tcttcaagaa gaagccctgg tccgtgaagg
    17101 acgagcgggg gcggctccgc gtcggggccg cgatcggcgc ggcgggcgac tacctcgagc
    17161 gcgccgcggc cctcgccgag gcggaggtgg acgtcgtcct gatggacgtc gcccacgccc
    17221 actccgagat cctcgcgaag gccgtccccg ccttccggag ccggttcccc cggatcccgc
    17281 tcgtcgtcgg caacgtggcg accgcggaag gggcgcgctt cctcgtcgac ctcggcgctg
    17341 ccgccgtgaa ggtcggcgtc gggccggggc gcggctgccg gacgcgcctc gagacgggcg
    17401 ccggcgtccc gcagctccag gcgatccgag aggcgtacct ggccacggaa gggaaggtgc
    17461 cgatcatcgc ggacggcgga atccggaacg acaaggacgt cttcctcgcc ctcgcctgcg
    17521 gcgcctcgac cgtcatgctc ggctcgctcc tctccgggac cgacgagtcc cccggcatcg
    17581 tcgtcgacga cccggcgacg cggcagaagg cgaagctcta ccgcggcatg acgtcccccg
    17641 aggccgtcgt cgacggctcg acggacgcgg gcgtcgccga cgccctcgcg acccccgccg
    17701 aagggcagtc ggtccgcgtg ccgtacgtcg ggagcgtcgt cgacgtcctc gcgcggatcg
    17761 ccggccacct ccgctccgcc gtcagctacg cgggcgagac gacgctcgcg gacgcgcacc
    17821 ggaagatcgc ggcggacccg gcccgctacc tcgtcccgct ctcggcggcc gcgaggacgg
    17881 agtccttcga gcgctgaagg aggaggacat ggcgattcac gtcgtccgcc tcggcgcccc
    17941 gcgaggcaga ggagaaggcc ttcggctcgg gacggtgcgt cgcccgccgc gcggcgtccc
    18001 gaaggcggag ttcgcctcgc gcgacttcta cgacgtctgg ctccccgacc tcgcccccag
    18061 cgaggagctc gtgaaggccg cgcaggcggc cgccgacgag agggcctgga aggcgttcga
    18121 gcggaagtac cgcgcggaga tgagcacgcc gcacgccgca cgtctcctgg acctcctcgc
    18181 cgccctctcg caccggacga gcctcgcggt cggctgctac tgcgccgacg aggcgcgctg
    18241 ccaccgctcg ctccttcggg cgctcctcgc cgagaggggc gccgagctcg cgtagcgcgt
    18301 ctccgaggcc gtccgctccg gtttccggcg agcttcgccg attccccatt cctggactta
    18361 acatcccgcc cacatgacgg cggaagaaca cgtgtgcccg cggtgcggca ttcgcttcga
    18421 cgcggacgag gagggtgtgt tcgccacctg cccggcctgc ggctcgctcc tccacctcgg
    18481 ggacgaggcg gagatcgccc accagcggct cgttcccgtc ctcgacgagg ctgcggtcct
    18541 cggacggctc gcgcgctggc tgagggaccg ggaggtcctg gccgatccga aggaggtcgc
    18601 cacgcgcctc ctctggttcc cgttctggct cctcccaggc gatgcgctcg tccccgcggc
    18661 gccgctcctc gcgagcaacc tcggcgcgtt tcgcgcgccg gccggcgatc gggtcgcctt
    18721 tcgggaggga gaggcgggac cggccgagat cgtcccggcc tccgtcccgc cggcggcgat
    18781 cgtcgacgac gaggcgaccc gccgcgcggc gaggctcctc cacgtgccgt tccgggaggt
    18841 ccgcttccgg ctctggggtc acgactacac ggtctggttc gacgcggcga cgggccaggc
    18901 tctcgatttc gacctgccgc cgacgtccga gcggcggctc gacgtgacct acgcgctcct
    18961 cctcctcgcc ctcttcgctg cgggcgtcta cggcgtccgg accctgttcg gcgggaccgc
    19021 cgcgcccggg tgggtgggcg cggccgtcct cgccggcgcc gccggggtct tcctggcggt
    19081 cgcgttcgcg ttgatccgcc tcacggagtc gcggtgacga acgcgcggcc taccgccctt
    19141 tcctgcccgc ggtgcggcgg ccggcttccg ggcgtggcct cttccgcgct cctgggctgt
    19201 ccgtcgtgcg actcgcccct tgcggtgctc gccgagggtt tcccggtccg cgagtcggtc
    19261 cggcccgcgc tcggcggcga cgaggcggtc gcccggacgc gcgccttctg ggagggacgg
    19321 aaggtgccgc gcgccttcct cgccgcaggc tccgtcgagg agccgatcct cgtcttcgtc
    19381 ccgttccacg agcgcgtacg gacgttcgtc cttcgcccca gggaggggcc ggtgttcgtc
    19441 gatcgcccct cgttcgcgcc ggccgtccgg attcccggcg tgcccctcga gaagctggat
    19501 gccgcctccg cgtcgggcgc gacggcgagg gaggccctga acgcctccgc gctccagcgc
    19561 cgcgccctcg tcttcgaccc gacggaggag gcgccgaaga ctcccgcgat ctcgggaggc
    19621 accgtcgtcg aggagaaggc cgggctcgtg tggctccccg tctggctcgt ccggtgccgg
    19681 cacgcccgca acctttacga ggcggtgctc gacggaacga gcgggtcggt tctccacgcg
    19741 cgtgcgccga tggagcggac gaccctcctg cccgaggccg tcttcttcac ctacgtcttc
    19801 gcgttcgtct tcgggatgcc gctcgcctgg tgggggaagc tcgtcgcgtt ctacttccag
    19861 ctgcattggc tcgggggcct cgtcctcacc gtggggacgc cgggcgtcgt cttctgggtg
    19921 ctggccttcg cgtgggaccg cgtccggttc cgctacgagt ggaccggcga ccgtctcggg
    19981 gtccggcgcg agccggtgaa ccggcccgga cggacgctcc ccgagaagat ccgggacggc
    20041 ttcttcaccg cggcccgctg gctcaacgga tcgccggaca aagaggaaga ccggtcgtga
    20101 gcggccgccc tcgcgtcgtc gcccggcgct gccccgactg cggctcgctc ctcctcgcga
    20161 cggccggcgt gcgctggatg acctgcaccg cctgcccctc cgccctcgac cccttcgccg
    20221 tgccggcgga gcggctcccg accttccgcc ccgccggagc cggagcggac gaggcgcgcc
    20281 ggctcgcctt ctacgtcttc gagagcgcgg ccaccgaggc gcccgcctgg atctggatcc
    20341 cggcgttccg gattctctcg acggacagcc ggtccgatcc cggcgccctg ctcgcgcgca
    20401 agggctaccg gcccgagctc gtcgccgcgc ccctcggctc cgccctcgcg cggacgccgg
    20461 atgaggcccg cgccctcgcg gcgctccgcc tcggcgcgaa agccgaggag gcggaccttc
    20521 gcgctgcccg cctcgtctcc ctctcctgcc gcgtctcgat ggggaacgtc gccgagaagg
    20581 tgtccggctt cgtcgcgccc gccgggagcc tccgcccgcc gccggcgtaa cggctcttca
    20641 ggctcccggg aggagagcga gcgtctccct ctgccgcgac gccggggccg cggagacctc
    20701 tcgcatcgcg aggcggcgcc ggaacaccag gtgccgccgc cgcgtcgctt cgacgagcgg
    20761 ccgccaggcc gacgcgacga ggcggtggcg gatgaggaag gtccagagcg ggttcgcgcg
    20821 tttgtagagg agcgacatgg cgaggtacgg cgcgacggcc ctcgggtcga gcggccggcg
    20881 gcgccagatc gacccggccg agaagaggcg ccggtagcag gaggcatagc cctcggcgag
    20941 ctcctccggg gtcatccgcg cggggcggaa gacgacgtgg gcggtgtcgt agagcgccca
    21001 gtcgcgatgg aggagccgcc ccccggcctc catccggcgg aagagcggcg tgccggggta
    21061 gggcgtcagg atgtggaacg tggcgcactc gagccggttc gcctcgatcc aggcgacggt
    21121 cctgtcgaag acgtcggggc cgtcgtggtc gaagccgagg acgaagctcc cgttcacctg
    21181 gatcccgttg tcgtgaagga tgccgacgcg gcgtgcgtag tcggcgaccg ggggcgtcct
    21241 cttccgcgcg tcggcgaggt tcgggtcgtt cagcgtctcg aagccgacga agacccccgt
    21301 gcagccggcg agggccatct cgcggacgag gcgcgggtcg tcggtgacgt cgatcgagac
    21361 ggcggcgctc cagatccgct cgagcggcgc gagcgcccgg cagaggcgga ggaggtgctc
    21421 cgggtctgcg ccgaggttgt tgtcgaggaa gacggcgtac ggctcgcccg tcgcgagcca
    21481 ctcgtcgacg acctgcgccg gctccttcac gctccacggc atcttcacgc ccttcgtcga
    21541 caggtagcag aagtcgcagc ggttgtggca gccgcgcgtc gccaggaggc tcgcggtcgt
    21601 caggaacgac tgccgcggga ggaggtcgcg ccgcggcggc ggggcatcgg cgtacggcgc
    21661 cgtgaaggcg gcgcggtaga cgggctcgag ggcgccgcgc tccgcgtcgg cgaggatccg
    21721 cggccagagg ggaacgccgt cgccgaccgc gagggcgtcg gcgtgcaggg ccgcctcgtc
    21781 ggggcaggaa gagacgtgga ggccgcccag gacgacgagc gcgccccgct cgcggtacca
    21841 gcgcgcgagc tcgtacgccc ggctcgcaaa ggtgaggtgg accgtgatgc cgacgacgga
    21901 cgggaacggc tcgtgcggcg gcggcccctg gaggaggttc tcgtcccaga gagcgaccac
    21961 ccagccggga ggtgtcgcgc ccgcgacgct cgtgagcgcg agcgtcggcg tcaggacgtg
    22021 cttgccgaag ctcgcgtgcg ggtctttcgg gtagaagggg ttgacgagga gggcgcggcg
    22081 cggtgtcggc gggcccggga cgagatccgg gtcgaggtgc ggcgcggcgc ggagctcagg
    22141 cggcgtgcga agcttcatgg ggatctccat tcgttgcggg cgccggggcc ggaaggccgc
    22201 cgaagcggcg ctcgcggccg gcgtaccagg cgagggcctc gtcgaggtcg ggggcgccga
    22261 cgtcgggcca gaggcggtcg ctgaagaaga gctcggcgta ggccgactcc cagaggagga
    22321 agtcgctcgt ccgccgctcg ccgccagtcc tcacgagcag gtcgacctcg ggcgccccgc
    22381 cctgccccgc cctcccgagc gccgcgagga gcgcttcgcg cgttggcgcg gggccggccg
    22441 gaaggagcgg gatcgccgcg aggacggcgc cccgccccga gtagtcgacc gcgatccgga
    22501 gctccatcgc cgccccgccc gccgtccggg cctcggccgc gtcgatcgcg gcgacgagcg
    22561 ccgggtcgag gcggtcgcgc cggccgatga cgcggaggcg gatcccgttc tccacgcagc
    22621 ggcgcgcctg ctcgccgagg gagcgctcga agaggcccat gagcgcctcc acctcggcgg
    22681 cgggcctccg ccagttgtcg gaggagaagg cgtagagcgt gagcgtgccg acgccgcagc
    22741 gccgagccgc ctcgacgacg cggcggacgg ccttcgcgcc ggcgcgatgc ccgacggccc
    22801 gcggaaggcc gcggcgcgtg gcccagcgcc cgttgccgtc catgatgatc gcgacgtgcc
    22861 gcgggagcgc cgccgcgcct cgagtcacgc tcctctctcc gctcccggac gcgccgtctg
    22921 tcttcatcgc tccacctttc gaaaagtact cggcattaca aagcaacgga acagacgaat
    22981 ccgcctctcg cattcctgcc tgcctcaggc cgggttccag cccgccttca gcggagagcc
    23041 ggacgcggaa tcctcgaggg cgcaggcctt cctcgcgtcc ttcagcacct tctcgagctc
    23101 gccgaggtag tcgaggaagc gccggcgccc ctcggcgctc agccgcacga gcgtctgcgg
    23161 acgccgcccc tcgaaccgct tccagacctc gacgaggccc gcctcctgga gcacctccag
    23221 atggcggttc aggttcccgt ccgtgagcgc gcagagccgc ttgaggtcgc cgaagaggag
    23281 cccctcgggg cgcgtcgcga gcgaggtcag gatgccgagc cgcgccttct cgtggaggac
    23341 ccggtcgagg ccttcgtagg cgaagcgccc ctcgttcgcg tcggcgctca ccgctccccc
    23401 ctcgggatgt cgaggtgcag gacgacggcc gccccgagga ggccggcgcc gaagacggcg
    23461 ccgtgcgcga ggccgagcgc cgcgagagtc gcgggaggga agagcaggag gcccgttccg
    23521 gcggagacga agtacagcgc cacccagccc gtcgcgcggg cgaggtacgg ccgggaggcg
    23581 aagacgccga ggccgaagag gagcgcccag agtccgggga ggagccggac gagcgcggac
    23641 cccgccgccg ggaggccgag ggtcacggcg gcccccgcga ggaggcatgg cacaaactgc
    23701 ccgacgaccc ggcgggtgtg ccggcgggcg aaggcgtctc cgcaccgggc gtagccggcg
    23761 acgatgaccg cggcgttgat cgcgacgttt ccgagcgcga cggcgatcca gtagccgaga
    23821 aagcgggctg cgtctccctc cgggacgagc ctcggctgga gccaccccgc gaggagcccg
    23881 acgacccccg cgatcgcgac cgggaccggc cggtaccctc ggaacacctc tcccttggcg
    23941 atctgcgcgt gaatctcctc gatccggtcg agcgcctggg ggacgtccac gccggagaga
    24001 ctactctgcg ttacagagtt gtcaagggga ctcgcggtac tgcaccggcg tcagccccgt
    24061 gaccgccttg aactggcggg tgaaggcgct ctggtcgcag tagccgcagg cgagggcgac
    24121 ccgcgcgatc ggcgcggcgc tctcgcgcag catccggcgc gccgcgtcga cccttacctt
    24181 gatgacgagc tgcgaggcag agaggccgaa gagcgcgcgg acacggcggg agaagcggct
    24241 cggcgagagc tcggcgagtc gcgcgagctc ttcgacgcgg tgcgggccgc cggggcgttc
    24301 gtggaggagg cggaggacgc ccgcgagccc ctcgaacccc gctccgggct ccggggaacg
    24361 gacgtcgcgc gacgtcccgg cgaggccggc gaccacgccg tcgggacccc ggatcgggac
    24421 cttcgtcgtg acgcaccatc cttcgtggcc gtcgggatag aggtggagct cgaggaggtc
    24481 ttccacgagg cgacccgtcc gacagacatg gaggtcctgg gcgaggtagc gctctccaag
    24541 cggtgccggg aagagctccc gcgccgtctt cccgaggagc tcccgcttcg ttcgccggcc
    24601 gagtcgccgg acgagcgtgt cgttcacggc ccggtagcgc ccctcggcgt ccttcgcgaa
    24661 gaagacgacg tccggaaggg cgtcgaagag ctccagggcg aatccagccg cggagagccc
    24721 gacctccagc gggtcggtcg cggacggcgc cgggcggcgg ggacgttgtg cggaaggcac
    24781 ggcggaaccc gccggaatcg tacaagacgc gccccagccc gcaggcggac gatctctgtg
    24841 aggaggcgtc atggccggcg cgccgcccgg gacggtcggg acgatcttca acgtccagcg
    24901 cgggtcgttc cacgacgggc ccggtatccg gacgacagtc ttcctgaagg ggtgccctct
    24961 ccgctgcccc tggtgccaca acccggaggg aattgccttc gcgccggagg tgctctggag
    25021 cgccgggcgc tgcctctcgt gcgggagctg cgcggcggcc tgcccgcgga acgccgggcc
    25081 tcttccggcc ggggccccgc tcggaagcga cgggtgctcg gcctgccgcg gctgcgtcga
    25141 ggcctgccct tccgccgcgc gagaggtggc cgggcgggag gtcaccgtcg cggaggtcgt
    25201 tgccgaggtc ctccgcgacc gggccgtcta cgaggagtcg ggaggcggcg tcaccttctc
    25261 cggcggcgag ccgctcgccc agcccgcgtt cctcctctcg gcgctcgcgg cctgccgccg
    25321 ggaagggctt cacgtcgtcg tcgacacctg cggcttcgcc gcgcgcgagg tcgccctcgc
    25381 ggcggccgac ctcgccgacc tcctcctctg ggacgtcaag acgctcgacc cgaagcgcca
    25441 cctgaagctg accggcgtct ggctcgcgcc gatcctcgag aacctcgccg ccgtgagccg
    25501 ggccggagcg aggatctggc tgaggctccc cgtgatcccc ggcgtgaacg acgacgacgc
    25561 gagcctcgcc cgcgccgccg cgctcgggga gcggacggcc ggcgtcgagc gcgtcagcct
    25621 cctcccctac cacacgaccg cgagcggcaa gcgggagcgc ctcgcgcgag cgcacgcgtc
    25681 cgccctcgcc gcggccgtct ccccgtctcc cgagcggatg gcgagcctca ccgccctctt
    25741 cgccccgacc ggaatcccgg tcacgatcgg aggatgagcc gatgaacccc cgcaccgccc
    25801 gtctccggca ggagagcctc gacgcgatcc cgacgatctc cgccgagcgt gcgcttctcc
    25861 tgaccgagta ctaccgcgag aacctcggcc gccattcgac gcctctcctg cgcgctcgcg
    25921 ccttccacga cctctgcgcc cggaagacgc tcttcctcgg cgagggcgag ctgatcgtcg
    25981 gcgaacgcgg gccccggccg aaggccgtcc cgacctaccc ggagctgacg tgccacagcg
    26041 tcgaggacct gcggattctc gattcccggc cgatgacctg gtacagcgtt ccccccgagg
    26101 tgatcgcggc gtacgaggag acggtcatcc cgttctggcg cggccggtcg atgcgcgacc
    26161 agatcttccc cgctctcccg cccgagtggc acgccgccta ctcggccggc gtcttcaccg
    26221 agttcatgga gcagcgcgcg ccggggcaca ccgtcctcga cgacaaggtc taccggaagg
    26281 ggatgaacgc cttccggcgc gagatcgccg ccgccctcgc cgccctcgac ttcgcgcgcg
    26341 acccggaggc cgtcgacaag cgcgaggagc tcctcgcgat ggacgtcgcc tgcgagtcgc
    26401 tcgtcctctt cgccgagcgg cacgccgcgc tggccgacga gcgggccgct ctcgagacgg
    26461 acgagatgcg tcgggccgag ctccggaaga tcgccgacgt ctgccggcgc gtcccggccg
    26521 agccgccgcg cgacttccac gaggcgctcc agatgtactg gttctgccac ctcggcgtcg
    26581 tcaccgagct gaacggatgg gacgccttca gcccgggtca cctcgaccag catctcctcc
    26641 ccttctaccg gcaggggctc gcaaacggga cgctgacgcc ggagagcgcc cgcgagctcg
    26701 tcgagtgctt cttcgtgaag ttcaacaacc acccggcgcc gccgaaggtg ggcgtgacgg
    26761 cggccgagag cgggacgtac accgactttg cgaacatcaa cctcgccggc ctcctcgccg
    26821 acggctccga cggctcgaac gaggtgacgc acctcctcct cgacgtgatc gacgagatgc
    26881 acctcctcca gccctcctcg aacgtccagg tctcgaggaa gacgccggag acggtgttga
    26941 agcacgtcct gcgcgtcgtg aggaagggct acggattccc ctcgatcttc aacgccgaca
    27001 cggtcgtcga agagcagctg cggcagggga agagcctcga ggacgcgcgg gccggcggct
    27061 gcagcg
//
LOCUS       DAOONL010000040        27032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1604_length_27032_cov_20.865181, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000040 DAOONL010000000
VERSION     DAOONL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_27032_cov_20.865181"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3031)
                     /locus_tag="PLP50_08035"
     CDS             complement(<1..3031)
                     /locus_tag="PLP50_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HPA51536.1"
                     /translation="MTKRIALGLVLAALLGSSPLLVAETTYLPGAAELSGLQGARFSS
                     TLELANPGDAAVTATVGLVPMAGMTTPAPVTRTLAAGESLRIEAALKTLFGLAEGSAG
                     TITVASETPLLASLTTRNVAAAEGPYGLGLGAVPEAELLATGETGHSIWVSHSADPKT
                     GYRTNLSVTLVDPGTVVVVRVFEADGSLAGETTLSAAAPQVWQQPVTAIAGEESLPVG
                     RAAFEVKAGRATAYAVVNDNVTSDAIALPCERVPAGSTERLVSGAALSPGHLGAFWVT
                     DLRLFNPGAEPVVATVRSLGAPSAASAPVPIPANGIVEVPRVLAFLGFPEKTASALLV
                     SADTPLLVAARTNNLDPTGVRTGTFSAQQFVSIWPTGLLAAGSTGYFTGIDQTLNVPG
                     TRTNLALVGGPEGAAGALVLRDERGAEQGRTPFQRAPGEWGQLGVADWFASALAGATS
                     RDGLSLATVPPNSRIDVTVEEGALDAYVSQIDNGSGDAVTRSFGLPGGGDCSKVAILT
                     LEATPLPVMPGIETTLSWTVAIQPPSANLTSQSIRIGSGPELQLDKDARSYKTSFPGA
                     GPVEVTLKVRKGSCVKTRTLQIAVCGDLALEPETLPVPTVGEPYPGVSFTAAAGRPPL
                     AFRISEGELPAGLTLTPSGFLGGTPVEAGAASFTVAVTDANGCTGERAYRIEVGCPPM
                     SIAPADLPAGTVGAPYPTVALTVTGGSGSGTFAGRRLPPGLTVSSSGRIEGTPTEAGQ
                     FDVEITYRDANGCDLTVARPILVCNPIGVTATLPAATAGVAIAPTTFTATGAVGPTTF
                     AVTAGALPTGVTFDGATGVLSGTPTVVGTFPFTVTATDSLGCTGTLATTLTVSCPAIT
                     VSPASLSNGTAGVAYGPATLTQTGGVGVVTYAVTTGALPAGLTLTSAGLLSGTPTVTG
                     TFSFTVTATDANGCTGTQSYTLVVECPVITVSPASLSNGTAGVAYGPVTLTQAGGVGA
                     VTYAVTAGALPAGVTLTTAGVLSGTPTVTGTFNFTVTA"
     gene            3358..4893
                     /locus_tag="PLP50_08040"
     CDS             3358..4893
                     /locus_tag="PLP50_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPA51537.1"
                     /translation="MSRRKLIGLGAATVAATALPDALFRLSSAAAATGPTEGVGYFAR
                     FGVTEKMLRDGLSAALSRGADYADLFFQHRVGNSLALEDGAVNRAYASVELGVGVRAV
                     KGDQTGYAYTEDLTPEALVQAAKTAAAIADGPAREGPKGLKVDTSLPARYPVKTRWED
                     VRPQQKLPLLSGLNEKVFAADKRITKVNVFFGDEAGAILIADSNGRVVEDLQPMTRLG
                     LSCVAEQDGKKETNGYNVAGRADLGYYSPDRLERIVKEAVSRTLVLFEAVQPPAGEMP
                     VVLGAGASGILLHEAIGHGMEADFNRKGISIFADRIGKPVANKFVNIVDEGTIPGARG
                     AINVDDEGNPVGKTMLVENGILTTYLHDSISAKHYKVKPTGNGRRESYAYAPVPRMRA
                     TYMLPGPHKKDEIVASVKKGIYCTNFTNGQVQIGAGDFTFYVKNGYLIEDGKLTKPIK
                     DVNIIGNGPKVLEKIDMVADDLVIDEGGWTCGKDGQSVPVSQGIPTVRVASITVGGRG
                     KQA"
     gene            4904..6232
                     /locus_tag="PLP50_08045"
     CDS             4904..6232
                     /locus_tag="PLP50_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPA51538.1"
                     /translation="MAQQDMLAAAHAAVKSALGKGAQEASARTYRVREVEVKWRDGAL
                     EKVHEATTRGLGLTLYVDGRYANVSTSDLRPEALETFIGDSVTLTRALAKDPFRTLPD
                     PKLYEGQAKVDLLLEDPKYATVTPEQRRAVAKEIEAAARSVKGSGAILSVTSSFSDTL
                     AESWRVSSNGFSGSRRDTQFWVSAEVSVKDPDGRKPEDYAFGGSRFVGEMPKAGEIGR
                     DAAERALSRLGAKKGESAVLTMAVENRAAGRLPAYLLGPLSGGALQQKRSFLEGKLGQ
                     AIASPALTLVDDPLVPKAFGSRLFDGEGIAAKRMPIVEAGVLKSYYIDTYYGKKLQMA
                     PTTGGPSNVVWTLGDKAQAALLAAMKEGILVTGFLGGNSNSLTGDFSLGVQGFRVRGG
                     KIAEPVGEMNISGNHLDLWKKLAAVGNDPYPYSSMKTPTLVFEGVQFAGV"
     gene            complement(7383..7775)
                     /locus_tag="PLP50_08050"
     CDS             complement(7383..7775)
                     /locus_tag="PLP50_08050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPA51539.1"
                     /translation="MTPRLPPPTFEYLNGEVLEQAPGRVRCLFRPTEEMTNPWGAVQG
                     GILAAFFDDTMGPAVVSIANGRGFTTVSLNVTYLRATKPGEPVVCDAIVVKHGRTQAY
                     AEATLTRQSDGELLARATSVNLFLEAPK"
     gene            complement(7780..9570)
                     /locus_tag="PLP50_08055"
     CDS             complement(7780..9570)
                     /locus_tag="PLP50_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPA51540.1"
                     /translation="MQKTLPALLLLLLVPMTIAAQTPTLADAKAFVEKAEEQLLKASI
                     AAERANWVASNFITEDTERIAAEGNQRRMELAAGLAKEATRFDGLALDAETARKLKLL
                     KLSLVLAAPSDPAKSEELARVAAGMEAAYGRGKWTPPGGESLSLEEISSRLATSRDPK
                     ELAALWAGWHEVGAGLRKDYARYVELGNAGAKELGFADMGAMWRSKYDMPPDAYAKEL
                     DRLWEQVKPLYVSLHAYTRMKLRETYGKDVVPEKGPIPAHLLGNMWAQAWGNLYPVLA
                     PKDADPGYDLTKILVEKKTDAKQMVRYGEGFFTSLGFEPLPKTFWERSLFTRPRDREV
                     VCHASAWCIDWVDDLRLKMCIEINAEDFATVHHELGHNVYQRAYNRQPVLFRDSANDG
                     FHEAIGDVIALSLTPEYLKKVNLLEKTPPPGKDVGYLLNMALEKIAFLPFGLLIDKWR
                     WQAFSGEVPEAKWNEAWWDLKLKYQGVVPPVARTEDHFDPGAKYHIPAGVPYSRYFLA
                     HVLQFQMHRALAKTAGCTDPIHTCSIYGNKEAGAKLIKMLEMGQSQPWPDALEALTGQ
                     REMDATAVLDYFAPLQQWLDEQTKGQPKGW"
     gene            9656..11581
                     /locus_tag="PLP50_08060"
     CDS             9656..11581
                     /locus_tag="PLP50_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPA51541.1"
                     /translation="MRLRVLVPAAVLAVLAVSTPGGRGAEPAASLAAFIRESYTKHEH
                     LVPTRDGTRLFTEVYVPKDAGPAKRYPFLMNRTPYSVAPYGIDAYPASLGPSEAAARE
                     KFIFVYQDVRGRMMSEGTFVDVRPVLPKKGPKDVDETTDAFDTVDWLVKNVPHNNGKV
                     GAWGISYPGFYAALAAIDAHPALVAVSPQAPVADWFAGDDFHHNGALFLAAAFNFYVR
                     FGQPREGPTTKWAPRFDHGTMDGYRFFLEAGSAAGLSKHMKGVAFWDDLMAHETYDAY
                     WQARNTRPHLAAIRPAVLTVGGWFDAEDAFGSLETYRAIERQSPGASNRLVVGPWWHG
                     GWARSDGESHGRVTFGQKTSVRYREEIELPFFLHHLKGAPDPKLPEATVFETGRNVWR
                     SFDAWPPREAKATSFRLGAGGVLASDPPPAGSAVAAEWVSDPAKPVPYVESVEIGMDA
                     DYMTADQRFASRRPDVAVWQTPPLEADLTVAGPIEVSLSVSTSGTDADWVVKLVDVWP
                     DDVPVEAWEWTPRNVWDEKPTRSKLGGNQQLVRGEPFRGRFRKSLSSPEPFVPGKVET
                     VAFTMPDVLHTFRRGHRVMVQVQSSWFPLVDRNPQTFVPVRTAGETDYRPATMRVHDG
                     AESPSRVVLRVLPPERP"
     gene            complement(11597..13375)
                     /locus_tag="PLP50_08065"
     CDS             complement(11597..13375)
                     /locus_tag="PLP50_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA51542.1"
                     /translation="MTEAGGTRSPRPFGILLEALLLAGLVTLSLAAVHAHQKDDSPTS
                     DEGIHLYSGAEALEDGSSWMNPEHPPLLKLAGAFALRPLGLRSPCGETPCAASPFGAY
                     PRWLYGNAAPAHVLVAAGRRPFPWVLAILVVALHLAVRPLAGPAAGLLAAGLVALDPT
                     FVAHAAYVHTDVGAALAFLAVVALAARAALTARLGPWLALGLALGLALVTKFSTALLV
                     PVVALLPLAPSLVRPGQGGGGPTPRGGLRDRGLAALAALGLAGLVVYGVYAYVLRAMP
                     AALVEASVRGYLSGRPARPDEVERYAALARAVPPIGHYVAGAKGVALLSERGRGANWF
                     RGEVSEKGFPLYFPAAFLLKSTSAVLVLLATAFVLGLARLRRSGTGGPSTTTAVLLAL
                     AVSAALLLASTRSAFNIGARHLLPIWALLVFAGAVVVGRELAARPRLRAALAGALVLS
                     AGLTLCSAGDSPIAWFNVLAGGPEGGRRWFSDSNVDWGQDLYRLHLYLSERGWEEETT
                     IVAFSGVATNYWSRRARVLDPAAPIRPGRYAVSHMMETLGEPFVREFEGEAAARQVAE
                     LVRALKARGRRVALVGGSITIWDLPG"
     gene            complement(13581..15500)
                     /locus_tag="PLP50_08070"
     CDS             complement(13581..15500)
                     /locus_tag="PLP50_08070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPA51543.1"
                     /translation="MASSSYTAADITVLEGLEPVRKRPGMYIGGVDSRGLHHLVWEIV
                     DNAVDEVINGYASRIEVRLGADGREVEVYDNGRGIPVDVHPRYKKSALELILTTLHAG
                     GKFEQGNYVHSGGLHGVGSSVVCALSSEMVVNVRRDGHEWRQSYAQGKATSKVTKVST
                     EKVRGSGTTVRFRPDPEIFGKLAFDADEIRDRLEAKTYLHRGLKIVFVDETKRPAVRE
                     EYAHDGGLADYLLKVVAERAKAPTHANGFVLAREADPRLELALQWTESTDEHLRSWVN
                     GIPTPSGGAHENGLKAGLVKAVRNFLGTHELLPKGVNPSAEDIREGLVALLSVYVLEP
                     QFQGQTKERLNNPEVAAQVDGAVRPAVENWLNENPSVAEQIVGRIVMATRAREASRAA
                     AQQVQRKTAISHRLNLPGKLADCSSTDPSESELFIVEGDSAGGSAKQGRDRQTQAILP
                     LRGKVLNAEQANSAKVAGNKELTDIVSALGCGSGADFDLGKLRYGKVFLLMDADADGH
                     HISTLLLTFFWRHLPKLVTEGHVYLAQPPLYRIDAGKETYWALDEAERDAVFAKLPKN
                     VKPEVMRFKGLGEMMPEQLKATTLDPRRRRALKVTVPDVLEAERTFADLMGKDAGARY
                     RFITERAREADALDV"
     gene            15593..16609
                     /locus_tag="PLP50_08075"
     CDS             15593..16609
                     /locus_tag="PLP50_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51544.1"
                     /translation="MPPGTDLLPRLFGLWVHLLERTGGAARLAALPVPIAPAARPLAA
                     LLAGAAAVLLLQAALASGRDVAVRAFRAAALLAALALPLAALRLVPASRGPAIGGAAL
                     LLVAFSSIAHRALASRRAEGTASRLLARARLLLEGTGLALSALSLGLLLAGRALPLRL
                     AFWSLFLLRLSIADLIDPSRLAARTGLAGSAARDARSALGKVRRPPRPLRRLRRALAA
                     AAKGSLLLLWLALPLAAALAPGEVAAGAWPRWALQLRWYPPLALALTALLLLGQAGRS
                     FRGGRPLDAARGAAVGVGTAAWLALAYRDGAFAAWRSALPGLVLAEAVAGFLLGAAAR
                     GRAR"
     gene            complement(16619..17041)
                     /locus_tag="PLP50_08080"
     CDS             complement(16619..17041)
                     /locus_tag="PLP50_08080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPA51545.1"
                     /translation="MSETVFTKIIRGEIPCHRVYEDERVFAFLDIAPLAPGHVLVVPK
                     EPAPTLDALSEESAAAIGRVLPRIARAVVAVTGTRDFNVLQNNGAAAHQAVPHVHFHV
                     IPKPSAGGGLGIDWPSGRLDGDEGRRIAAELAARLANG"
     gene            complement(17038..19806)
                     /locus_tag="PLP50_08085"
     CDS             complement(17038..19806)
                     /locus_tag="PLP50_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA51546.1"
                     /translation="MTAEPSSSIPPGTLVGRYRILASVGSGAMGEVYRAEDTSLGRIV
                     ALKTLPLDLVGTPDRRIRFGNEGRVMASLSHPNLVQVFDVGEFDGVPYLVQEFVEGET
                     LEGILLGGAVPAPKAARWIAQAAEGLAAVHDAGFLHRDIKPSNLMVDRQGRIRVLDFG
                     LAKVQSVPSDERKSQPSLTTDGFVVGTAQYMSPEQASGKKADARSDVFSLGAVFYELL
                     AGKAPFGGETAVDVMYAILHLPVPPIPGFETGVLRGFAAIADRALAKEPDERFPSMRD
                     LAAEIGAVLEEGGWEDEAAPKRPSTGRLSTIPPPAPPFAKRPGVRLAAGIGAALLALV
                     AAGLYLFRGRGEEAPAAALYRPVQLTTSTGLDVFPAFAPDGRTIVYASDRTGRFELYR
                     LPLAPGGREIALTTDGRQNLSPAWSPDGETIAYHAKSVGGVWLIPALGGVPRQLTTFG
                     SRPSFAPDGSALVLESGSIVDFAANSLGALPPSVLWIAPLDGAPPRPLTRVGRPSGGH
                     GAASWSPDGKRIAFAAYDRTSSEIWVVGVDGEDLVQVSEGNGQCYDPVWSADGGSITY
                     PCRSVDFAAGLYRRRIDPATGRTTGAPEEVATLGSAGTAFVKQLAISRDGTRLAWAGL
                     SMASNVWKVPVDGRGERTANEQAVTRETGRNSRPALARDGKRIALSRWRTGANQDVWI
                     VDADGGNAVQRTTDPGDDDYPFWFPGDARLAFMSQRRGHRALFTLDLTTGREELLADF
                     GPGHDAMRLSPDGTKVAFHSNRGGTTLNVWVAELPAGTPRQVTFDRELAAFPCWSPDG
                     RTLAFEAKREGDIHVFTVPVSGGTPVQLTSGSGQSWPYSFSPDGSKVAFAALRDGVWN
                     VAWVTRDGGTVRIVTANERLNSYIRYPEWSPTGEAIYYELAETTGNIWLVDTAAKDAA
                     GEKGGGRP"
     gene            19944..21035
                     /locus_tag="PLP50_08090"
     CDS             19944..21035
                     /locus_tag="PLP50_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPA51547.1"
                     /translation="MPAGRRLLVPLLLAALVATPSCRRLRSATSEAEPRPVTARGDLA
                     ADERATIDLFRQSSPSVVFITTLSRRRTGLFRIAEIPRGEGSGFVWDADGHVVTNFHV
                     IQGASSARVTLADGSTWPASFVGGAPDRDLAVLRIFPTGRTLRPILIGTSKDLLVGQK
                     VFAIGNPFGLDQSLSTGIVSALGRSIESVTGRQIEGVIQTDAAINPGNSGGPLLDSAG
                     RLIGVNTAIASTTGSSAGIGFAVPVDTVNQVVPQLIQHGRVIRPRLGVTLADDAVAAR
                     LGVEGALILSVEPDTGAAEAGLRGTTRDEDGSLLLGDVVTRAGERPVRSADDLIAALE
                     EKKPGETLPLLVLRAGETVRVAVSLSAAP"
     gene            complement(21032..24268)
                     /locus_tag="PLP50_08095"
     CDS             complement(21032..24268)
                     /locus_tag="PLP50_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA51548.1"
                     /translation="MLRYRTALLAVCLLASSAAPALDPGKRLSQYGLDVWQEGDGLPQ
                     NTVQAVLRGRDGYLWLGTQEGLVRFDGARFTTFDRRNTPELKHNSVISLAEGPDGTIW
                     AGTNGGGVLCRRRDGTFSAIGTAEGLAGGIVWSIAFDRAGDAWVAGDGIGAQRVVDGR
                     GAERIGRAQGLPLEQVRAIAFDADGALWVGTTGAGAAVVKGGRVTKVWDAATLGTGLV
                     RGLAPDAGGGVWVATSGGGLTYLKGDAVVRYREADGFPADQVSAVHVDAGGTLWFGTW
                     GAGVGRLRNGVPEVIGTREGLSNDQVWSLTSDEEGSVWIGTWVGGLNRLRDGLFLTYT
                     TREGLTSDNVRAVCPGRDGSVWIATAGGGLNRLKEGRVTAYREADGLPSDHVSALLED
                     RRGRLWVGTNLAGAAVFERGRFRPLGPAQGLPHHDVRAFLEDREGNVWIGTIGGGLTR
                     VAPDGSLRVFTVARDGLPTDRVVALAEAPDGALWIGSSGGGAVRYGEGRFRAFTTREG
                     LTSNRVLTVFPDAGGAVWIGTSGGGLNRIRGETVTSVTTREGLFDDLVQVILDDGKGS
                     LWMTCNKGVFRVEREELEAVADGRKAMLRPFALGASDGMRSASAAGGQQPAGFVAADG
                     RLWFPTFRGLAVLDPARIPASGRTPPVAIEEVLVDGRPVDLRVPLVLPPRAERVEIHY
                     TALTLVAPDRVRFRIYLDGLDDEPLEVGTRRVAYYTNLPSGPYVFRVSAADAGGAWLG
                     RGATFSFRKKARAREAWWFWVGLLGAAAGATWGGLKWRIASLRARESELLALVAERTR
                     SLSEEKTRAEEALVRAEEARAEAERHREVAEKATAEAEEASRTKSRFLANVSHELRTP
                     LNAIIGYSEMLSEEAQEEGRQALGRDLEKIRSAALHQLELINSVLDLARIEAGRLDLD
                     VETFLVRPLVEETVAIVRPLLERNRNSLVVRFSPEAAFVMTSDAMKVRQCLFNLLSNA
                     CKFTQDGRVEVDVALVEEAGRPWASFRVRDDGIGITPEKAARLFEPFAQADAATAQRF
                     GGSGLGLSITRELCLLMGGRVTVESVPDEGSVFEIRLPAALPRPPAAA"
     gene            24350..25585
                     /locus_tag="PLP50_08100"
     CDS             24350..25585
                     /locus_tag="PLP50_08100"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPA51549.1"
                     /translation="MRREAKGFRVIETTGVPVKAWTLGVPCDPAAEKQLANAASLPIV
                     YRWVAAMPDVHVGIGATVGAVIPTVEAIIPAAVGVDLGCGMAASLTSLTANDLPTNLA
                     PLRDAIEKAVPHGRTDSGGRSDRGAWHDPPARIGEAWRLLEPGWRAILDARPELDRGN
                     HPARHLGTLGTGNHFIELCLDEAGRVWLLLHSGSRGVGNRIGTDYIEVAREEMRVHQR
                     NLPDRDLAWLTEGTESFENYVAAVSWAQEYARTNRDLMLVAIEEALASVPSLPPFRSR
                     LLAVSCHHNYVRRERHYGREVWVTRKGAVRAGKGELGIIPGSMGARSFIVRGLGNPES
                     FESCSHGAGRAFSRNEARRRFTLADHAEATAGVECRKDEAVLDETPAAYKPIEAVMNA
                     QKDLVEILHTLKQVVCVKG"
     gene            complement(25707..26495)
                     /locus_tag="PLP50_08105"
     CDS             complement(25707..26495)
                     /locus_tag="PLP50_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA51550.1"
                     /translation="MMRPETPAALRLLALHAFPLDGRLWGLVSAAAASGQLGEGVAVS
                     APDFRGRGKSGLSAEKVHGMPLLSKDGAALLRPDEPFVVMGMSMGGYVALELIARLGP
                     ELRPRLRGLVLCNSRANADDEAGKAKRAATAEAIEKDGMEPLLSAMLPQFLAPSSRGG
                     ALEQLIRTMFLATPPATAAADQRGMAERADRFDVLGTLAVPFLAAVGEDDPLTPPSAS
                     EAMVEAAGNAPYVELLTLPGAHMAPLEHPEEFLPKLRAFLDRCR"
     gene            26559..>27032
                     /locus_tag="PLP50_08110"
     CDS             26559..>27032
                     /locus_tag="PLP50_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51551.1"
                     /translation="MPASRAHAAPVALLLVALLAPRPLLGDDLFAQLGDAARDGRLEA
                     EARLREGSVPVMAGYSVTVKGAPATRLGVAVREGKLESLRYVAPDDTFLLVGRGLRPD
                     VVLTRIETDPSGTITALDFHGRGLGRLVVGLFRPLVRRKVRQMKLHTELAELFRGN"
BASE COUNT         3759 a         9977 c         9572 g         3724 t
ORIGIN      
        1 tcgccgtgac cgtgaagttg aacgtccccg tcaccgtcgg cgtccccgag aggacgcccg
       61 ccgtcgtcag cgtcacgccc gccggcagcg ccccggccgt cacggcgtac gtcaccgccc
      121 cgactccgcc cgcctgcgtc agcgtcactg gcccgtatgc caccccggcc gtcccgttcg
      181 aaaggctcgc cgggctcact gtgatgaccg ggcactccac cacgagcgtg taggactgcg
      241 tccccgtgca cccgttcgcg tcggtcgccg tcacggtgaa gctgaaggtc cccgtgacgg
      301 tcggcgtccc cgagaggagc ccggcgctcg tcagcgtcag tccggccgga agcgctccgg
      361 tcgtgacggc gtacgtcacc accccgactc cgcccgtctg cgtcagcgtc gccggcccgt
      421 aggccactcc cgccgtcccg ttcgaaaggc tcgccggact gaccgtgatc gccggacagc
      481 tcaccgtgag cgtcgtcgcg agggtcccgg tgcagccgag agagtcggtc gcggtgacgg
      541 tgaaggggaa cgtcccgacg acggtcggcg tgccggacag gactcccgtg gcgccgtcga
      601 aggtgacccc cgtcgggaga gcgcccgcgg tgaccgcgaa cgtcgtcggg ccgaccgcgc
      661 cggtggccgt gaaggtggtc ggcgcgatcg ccacgccggc cgtcgccgcc ggaagggtcg
      721 ccgtgactcc gatggggtta cagaccagga tcgggcgcgc gaccgttagg tcgcagccgt
      781 tcgcgtcgcg ataggtgatc tcgacgtcga actggcccgc ctcggtcggc gtgccctcga
      841 tccggccgct gctcgagacc gtgaggcccg gcgggaggcg gcgccccgcg aacgtgcccg
      901 aaccggagcc cccagtgacg gtcagggcga ccgtcgggta gggagctccg accgtgccgg
      961 ccggaaggtc cgcgggcgcg atcgacatcg gcgggcagcc gacctcgatc ctgtaggcgc
     1021 gctctccggt gcagccgttg gcgtccgtga cggcgacggt gaacgaggcc gccccggcct
     1081 cgaccggcgt ccccccgagg aagcccgacg gggtgagggt gaggccggcg gggagctcgc
     1141 cttccgagat ccggaaagcg agcggcggac ggccggccgc ggccgtgaag gagacccccg
     1201 ggtacggctc gccgaccgtg ggcacgggga gcgtctcggg ctcgagcgcc aggtctccgc
     1261 agaccgcgat ctggagcgtc cgcgtcttca cgcagcttcc cttccggacc ttcagcgtca
     1321 cctcgacggg gccggctccc gggaaggagg tcttgtacga ccgggcgtcc ttgtcgagct
     1381 ggagctcagg gccggagccg atccggatcg actgggaggt caggttcgcg gacggcggct
     1441 ggatcgcgac ggtccacgag agcgtcgtct cgatgcccgg catcaccgga aggggcgtcg
     1501 cctcgagcgt gaggatggcg accttcgagc agtcgcctcc tcccgggagg ccgaacgagc
     1561 gcgtgacggc gtcgcccgag ccgttgtcga tctggctcac gtacgcgtcg agcgcgccct
     1621 cctcgaccgt gacgtcgatg cgcgagttcg gcgggaccgt ggcgagggag agcccgtcgc
     1681 gcgaggtcgc gcccgcgagg gccgacgcga accagtcggc gacgccgagc tggccccatt
     1741 cgccgggtgc cctctggaac ggcgtccgcc cctgctcggc tccgcgctcg tcccggagga
     1801 cgagggcccc ggccgccccc tcggggccgc cgacgagcgc gaggttcgtc cgcgtcccgg
     1861 ggacgttcag cgtctggtcg atccccgtga agtagccggt cgagcccgcg gcgaggagcc
     1921 ccgtcggcca gatcgagacg aactgctgcg ccgagaaggt ccccgtccgg actccggtcg
     1981 ggtcgaggtt gttcgtccgc gccgccacga ggagcggcgt gtccgcggag acgaggaggg
     2041 cgctcgccgt cttctcgggg aagccgagga aagcgaggac ccgcggcacc tccacgatcc
     2101 cgttcgcggg aatcgggacg ggcgcgctcg ccgccgaggg cgcgccgagg gagcggaccg
     2161 tcgcgacgac cggctcggcg cccgggttga agaggcgcag gtccgtgacc cagaaggcgc
     2221 cgaggtggcc cggcgagagg gcggcgcccg agacgagacg ctcggtcgag cccgcgggga
     2281 cccgctcgca cgggagcgcg atcgcgtcgg aggtgacgtt gtcgttgacg acggcgtagg
     2341 ccgtcgcccg gccggccttc acctcgaacg ccgcgcggcc gaccggaaga gactcctcac
     2401 cggcgatcgc cgtcaccggc tgctgccaga cctgcggggc cgcggccgag agggtcgtct
     2461 cgccggccag gctcccgtcg gcctcgaaga cgcggacgac gacgaccgtt cccgggtcca
     2521 cgagggtcac ggagagattc gtccggtagc cggtcttcgg gtcggccgag tggctcaccc
     2581 agatcgaatg ccccgtctcg ccggtcgcga gaagctccgc ctccggcacc gcgccgaggc
     2641 ccagcccgta aggcccctcg gccgcggcga cgttgcgcgt cgtgaggctc gcgaggagcg
     2701 gtgtctccga agcgaccgtg atcgtgccgg cgcttccctc cgcgaggccg aagagcgtct
     2761 tcagggcggc ctcgatccgc aggctctcgc ccgccgcgag cgttcgcgtg accggagcgg
     2821 gcgtcgtcat gccggccatc gggacgagcc cgaccgtcgc cgtcaccgcg gcgtcacccg
     2881 ggttcgccag ctcgagggtc gaagagaatc gcgcgccctg gaggccggag agctcggccg
     2941 cgcccggcaa gtaggtcgtc tccgcgacga ggagcggcga cgagccgagg agggcggcca
     3001 gaacgagccc gagggcgatg cgcttcgtca tctttcttga cctcatggaa tcagcaggga
     3061 gcttcgattc agggaggggc tggcgctggg acgaggggcc ggagagctca gcgaagagcc
     3121 gggagcagga cggccacgag gcctggaggt ctggactaga cgggggacca gacggacgct
     3181 accacggtca ggttcgaaca cggatcgatt ctcaatgtaa tccctttccg gtccggaacg
     3241 aaactttcgg gcgcatcggg cgtagccgct atatgtgccg caccggcgcg gtgtacgaac
     3301 tgtgaccttg cgtcacactt ttcgaggagg acttgcccca tgcctgagaa tcccggcatg
     3361 tcgagacgaa agctcatcgg cttgggcgcg gcgaccgtag ctgccaccgc cctgcccgac
     3421 gccctcttcc gcctctcatc cgccgccgcc gcgaccggcc cgacggaggg agtcggctac
     3481 ttcgcccggt tcggcgtcac ggagaagatg ctccgcgacg gcctctccgc cgccctctcc
     3541 cgcggggccg actacgccga cctcttcttc cagcaccgcg tcggcaacag cctcgccctc
     3601 gaggacggcg ccgtgaaccg cgcctacgcg agcgtcgagc tcggcgtcgg cgtgcgagcc
     3661 gtcaagggcg accagaccgg ctacgcctac acggaagacc tcacccccga ggcgctggtc
     3721 caggcagcga agaccgccgc cgcgatcgcc gacggtccgg cgagggaagg gccgaagggg
     3781 ctgaaggtcg acacctccct gccggcccgc tacccggtga agacccgctg ggaggacgtg
     3841 cggccgcagc agaagctgcc gctcctctcg ggcctgaacg agaaggtctt cgccgccgac
     3901 aagcggatca cgaaggtgaa cgtctttttc ggcgacgagg cgggggcgat cctgatcgcc
     3961 gactcgaacg gccgcgtcgt cgaggacctc cagccgatga cgcgcctcgg cctctcctgc
     4021 gtcgcggagc aggacgggaa gaaggagacg aacggctaca acgtcgccgg gcgcgccgac
     4081 ctcggctact actcccccga ccgcctcgag cgaatcgtga aggaggccgt ctcccgcacc
     4141 ctcgtcctct tcgaggcggt gcagcccccg gccggcgaga tgccggtcgt cctgggggcc
     4201 ggcgcttcgg gaatcctcct ccacgaggcg atcggccacg ggatggaggc ggacttcaac
     4261 cggaagggga tctcgatctt cgccgaccgg atcggcaagc cggtcgcgaa caagttcgtg
     4321 aacatcgtcg acgaggggac gatcccgggc gcccgcggcg cgatcaacgt cgacgacgag
     4381 gggaacccgg tcggcaagac gatgctcgtc gagaacggca tcctgacgac ctacctccac
     4441 gactcgatct ccgcgaagca ctacaaggtg aagccgaccg gcaacgggcg ccgcgagagc
     4501 tacgcctacg ccccggtccc ccggatgcgc gccacctaca tgctccccgg accgcacaag
     4561 aaggacgaga tcgtcgcctc ggtcaagaag gggatctact gcacgaactt cacgaacggg
     4621 caggtgcaga tcggggccgg cgacttcacg ttctacgtga agaacggcta cctcatcgaa
     4681 gacggcaagc tcacgaagcc gatcaaggac gtgaacatca tcgggaacgg cccgaaggtc
     4741 ctcgagaaga tcgacatggt cgccgacgac ctcgtcatcg acgagggggg ctggacgtgc
     4801 ggcaaggacg gacagtcggt gccggtctcc caggggatcc ccaccgtgcg cgtcgcgtcg
     4861 atcaccgtcg gcggacgcgg aaagcaggcg tgaggtgaga gagatggccc agcaggacat
     4921 gctcgccgcc gcccacgccg ccgtgaagag cgccctcggc aagggggcgc aggaggcctc
     4981 cgcccgcacg taccgcgtcc gcgaggtcga ggtgaagtgg cgcgacggag cgctcgagaa
     5041 ggtccacgag gcgacgaccc ggggcctcgg cctcacgctc tacgtcgacg gccgctacgc
     5101 gaacgtctcg acgagcgacc tccgcccgga ggcgctcgag acgttcatcg gcgactccgt
     5161 caccctgacc cgcgccctcg cgaaggaccc gttccggacg ctccccgacc cgaagctcta
     5221 cgaggggcag gcgaaggtcg acctcctcct cgaggacccg aagtacgcga ccgtcacccc
     5281 cgagcagcgc cgcgccgtcg cgaaggagat cgaggccgcc gcccgctccg tgaaggggtc
     5341 cggcgcgatc ctctccgtca cctcgagctt cagcgacacg ctcgccgaga gctggcgcgt
     5401 ctcctcgaac ggcttctccg gatcgcgccg cgacacgcag ttctgggtct ccgccgaggt
     5461 gagcgtgaag gaccccgacg gccgcaagcc ggaggactac gccttcggcg gctcgcgctt
     5521 cgtcggcgag atgccgaagg cgggcgagat cggccgcgac gccgccgagc gcgccctctc
     5581 gcgcctcggc gcgaagaagg gggagtcggc cgtcctcacg atggcggtcg agaaccgcgc
     5641 cgcgggccgg ctcccggcct acctcctcgg gcccctctcc ggaggcgcgc tccagcagaa
     5701 gcgctccttc ctcgagggga agctcggcca ggcgatcgcg agccccgcgc tcacgctcgt
     5761 cgacgacccg ctcgtcccga aggcgttcgg ctcgcgcctc ttcgacggtg aggggatcgc
     5821 cgcgaagcgg atgccgatcg tcgaggcggg cgtcctcaag tcgtactaca tcgacaccta
     5881 ctacgggaag aagctccaga tggcgccgac gaccggcggc ccgtcgaacg tcgtctggac
     5941 gctcggcgac aaggcccagg ccgcgctcct cgccgcgatg aaggaaggga tcctcgtcac
     6001 cggcttcctc ggcggcaact cgaacagcct cacgggcgac ttctcgctcg gcgtccaggg
     6061 cttccgcgtc cgcggcggca agatcgccga gccggtcggc gagatgaaca tttccggcaa
     6121 ccacctcgac ctctggaaga agctcgccgc cgtcggcaac gacccctacc cctactcctc
     6181 catgaagacc cccaccctcg tcttcgaggg cgtccagttc gccggggtct gaccgggaaa
     6241 atggccgcgt agagcggccg gagacacgat caccgagggc cggtccgaaa gggccggccc
     6301 tcttctcgtc cggggggcgg tgtcaagcgc cggcggcgag acacgggaca cgaaggacga
     6361 aggacgaagg acgagaggga cgaccgacgc atccacttcg ccgtaccgca cgagccgcgg
     6421 gcgggaggga tatcccgcgg cgctcgccgg ggggcctgag cttcgcgcgg ccccccggac
     6481 cccccactcg caatctggac ttcgctgctc ttcagaggcc tccgctgcgc gcgcgcgacc
     6541 cgcatcggtc ccgctgcgcg ggccgccggc gagcgcgcaa gctcctcgct ccgctcgtcg
     6601 aatcggtgcg cgctctcacc ccggcggcct gctcgcggaa ccgtgcgggt accccgcgcg
     6661 tgctcgaacg ctcgctcgcg ggacacccct cccgcccgct cacgtccagg gttgctacag
     6721 accacgtgcg cgagacacat tggcctcccg caagcagcgc ccgcgatcgg aggggggccg
     6781 tcagccctcg tcgcggtgct tcgggcccgg gttccggacc tcgtaggtcg ccgacgcgtc
     6841 gctgcccgcg atcagcgggt accgagcctg ctcccggatc gggtcgaggc tcaggtccac
     6901 gtccaggtcc ggccagcaga ggtggtcctc cgagagcctc tggacgttca ggatcgcagc
     6961 ccacagggcc tttcgcgaac cacgggacct ggtcgagcga gaggaagaac tctccctcct
     7021 tgagaagcac ccagatgcca tgcctcgagt gcagtcttcg cggtccgcac ctgaacctct
     7081 tcggagaggt cgacatcacc acggccgcgg gcgaggcgga gcgtctcgga ggcgaccccg
     7141 agcacggccg gggacccgct cgcggtcctg aggaccgggc ggggagacag cccgcgcacc
     7201 gattcgagcg aagcgagctt gcgcgggcgc cccgcccggc cgcagggaac gcgcaagcgg
     7261 gtcggtcgcg cgcagaagga gccgaagaga cgccccgccc cgtccgcggc ccccggccga
     7321 agcgacgaga gggcggagaa gggacttcac cggtctcctg tctcggctct cccggactac
     7381 ccctacttcg gcgcctcgag gaagaggttg accgaggtcg ccctcgcgag gagctcgccg
     7441 tcggactggc gggtgagggt cgcttcggcg taggcttggg tgcggccgtg cttgacgacg
     7501 atcgcgtcgc agacgacggg ctcgcccggc ttcgtcgcgc ggaggtaggt cacgttgagc
     7561 gagacggtcg tgaacccgcg gccgttcgcg atcgagacga ccgcgggtcc catcgtgtcg
     7621 tcgaagaagg cagcgaggat cccgccctgg acggcgcccc aggggttcgt catctcctcc
     7681 gtcgggcgga agagacagcg gacccggccg ggcgcctgct cgaggacctc gccgttgagg
     7741 tactcgaacg tcgggggcgg gagcctcggc gtcatccctc taccagccct tcggctgccc
     7801 cttcgtctgc tcgtcgagcc actgctggag cggcgcgaag tagtcgagga cggccgtcgc
     7861 gtccatctcg cgctggccgg tgagggcctc gagcgcgtcg ggccacggct ggctctgccc
     7921 catctcgagc atcttgatca gcttcgcccc ggcttccttg ttgccgtaga tcgagcaggt
     7981 gtgaatcggg tcggtgcagc cggccgtctt cgcgagggcg cggtgcatct ggaactggag
     8041 gacgtgggcg aggaagtagc gcgagtaggg gacgcccgcc gggatgtggt acttggcgcc
     8101 cgggtcgaag tggtcctcgg tgcgcgcgac gggcgggacg accccctggt acttcagctt
     8161 gaggtcccac caggcctcgt tccacttcgc ctccggcacc tcgccggaga aggcctgcca
     8221 ccgccacttg tcgatgagga ggccgaacgg caggaaggcg atcttctcga gcgccatgtt
     8281 gaggaggtag ccgacgtctt tcccgggagg aggcgtcttc tcgaggaggt tcactttctt
     8341 caggtactcg ggcgtcagcg agagggcgat gacgtcgccg atcgcctcgt ggaagccgtc
     8401 gttcgcgctg tcgcggaaga ggacgggctg gcggttgtag gcgcgctggt agacgttgtg
     8461 cccgagctcg tggtggacgg tcgcgaagtc ctccgcgttg atctcgatgc acatcttcag
     8521 gcgcaggtcg tcgacccagt cgatgcacca ggcgctcgcg tggcagacga cctcgcggtc
     8581 gcgcgggcgg gtgaagagcg accgctccca gaacgtcttc gggaggggct cgaagccgag
     8641 cgaggtgaag aagccctcgc cgtagcggac catctgcttc gcgtcggtct tcttctcgac
     8701 gaggatcttc gtcaggtcgt agcccgggtc ggcgtccttc ggggcgagga ccgggtagag
     8761 gttcccccac gcctgcgccc acatgttccc gaggaggtgc gccgggatcg gccccttctc
     8821 ggggacgacg tccttcccgt acgtctcgcg gagcttcatc ctcgtgtagg cgtggagcga
     8881 gacgtagagg ggcttcacct gctcccagag ccggtcgagc tccttggcgt aggcgtcggg
     8941 cggcatgtcg tacttggacc gccacatcgc gcccatgtcc gcgaagccga gctccttcgc
     9001 gccggcgttc ccgagctcga cgtagcgggc gtagtccttc cggaggcccg ccccgacctc
     9061 gtgccagccg gcccagaggg cggcgagctc cttcgggtcg cggctcgtcg cgaggcggct
     9121 cgagatctcc tccagggaga gcgactcgcc gccgggcggc gtccacttgc cgcggccgta
     9181 ggccgcctcc atcccggcgg cgacccgcgc gagttcctcg ctcttggcgg gatcggacgg
     9241 cgccgcgagg acgagggaga gcttcaggag cttcagcttc cgggccgtct ccgcgtcgag
     9301 cgcgagcccg tcgaagcggg tcgcctcctt ggcgagaccg gcggcgagct ccatccggcg
     9361 ctggttcccc tcggcggcga tccgctcggt gtcctcggtg atgaagttcg aggcgaccca
     9421 gttcgcgcgc tcggcggcaa tcgatgcctt gaggagctgc tcttcggcct tctcgacgaa
     9481 agccttcgca tcggccaggg tcggggtctg ggccgcgatc gtcatcggga cgaggaggag
     9541 aaggagaagg gcggggaggg tcttctgcat gtggggttca ctccgatcga cgatcatccc
     9601 acgcgagcgg tccgctgtta acgttcgccc gagcgccccc gagggaggtc ccgtcatgcg
     9661 ccttcgcgtc ctcgtcccgg ccgccgtcct cgccgtcctc gccgtctcca cgcccggcgg
     9721 gcggggcgcg gagcccgcgg cgtcgctcgc cgccttcatc cgcgagagct acacgaagca
     9781 cgagcatctc gtcccgactc gcgacgggac gcgcctcttc acggaggtct acgtccccaa
     9841 ggacgccggg cccgcgaagc gctatccgtt cctgatgaac cggaccccgt attcggtcgc
     9901 tccgtacggg atcgacgcct atccggcctc gctcgggccg tcggaggccg cggcgcggga
     9961 gaagttcatc ttcgtctacc aggacgtgcg gggccggatg atgtcggagg ggacgttcgt
    10021 cgacgtgcgg cccgtcctcc cgaagaaggg gccgaaggac gtcgacgaga cgacggacgc
    10081 cttcgacacg gtcgactggc tcgtgaagaa cgtcccccac aacaacggaa aggtgggggc
    10141 ctgggggatc tcctaccccg gcttctacgc ggccctggcg gcgatcgacg cgcacccggc
    10201 gctcgtcgcc gtctcgccgc aggccccggt cgccgactgg ttcgcgggcg acgacttcca
    10261 ccacaacggc gcgctcttcc tcgcggcggc gttcaacttc tacgtccgct tcggccagcc
    10321 gcgggagggg ccgacgacga agtgggcgcc ccggttcgac cacgggacga tggacggcta
    10381 ccgcttcttc ctcgaggccg gttcggccgc cggcctctcg aagcacatga agggggtcgc
    10441 cttctgggac gacctgatgg cccacgagac gtacgacgcg tactggcagg cgcggaacac
    10501 gcgaccgcac ctcgccgcga tccgccccgc ggtgctgacg gtcggcggct ggttcgacgc
    10561 cgaggacgcc ttcggctcgc tcgagaccta ccgggccatc gagaggcaga gccccggcgc
    10621 gagcaaccgg ctcgtcgtcg gcccctggtg gcacggcggc tgggcgcgga gcgacggcga
    10681 gtcccacggg cgcgtcacct tcggtcagaa gacctccgtt cgctaccgcg aggagatcga
    10741 gctcccgttc ttcctccacc acctcaaggg cgctcccgac ccgaagctgc cggaggcgac
    10801 cgtcttcgag acggggcgga acgtctggcg ctccttcgat gcctggcctc cgcgcgaggc
    10861 gaaggcaacc tccttccggc tcggcgcggg gggcgtcctc gcttccgatc ctccgcccgc
    10921 cgggagcgcc gtcgcggcgg aatgggtcag cgaccccgcg aagccggtgc cgtacgtcga
    10981 atccgtcgag atcgggatgg acgccgacta catgacggcc gaccagcgct tcgcctcgcg
    11041 ccgacccgac gtcgccgtat ggcagacgcc gccgctcgaa gcggacctca ccgtcgccgg
    11101 cccgatcgag gtgagcctct ccgtctcgac gagcggcacc gacgccgact gggtcgtgaa
    11161 gctcgtcgac gtctggcccg acgacgttcc cgtggaggcg tgggagtgga cgccgcggaa
    11221 cgtctgggac gagaagccga cccgctcgaa gctgggcggc aaccagcagc tcgtccgggg
    11281 cgagccgttc cgggggcgct tccggaagag cctctcctcg cccgagccgt tcgtccccgg
    11341 gaaggtggag acggtcgcct tcacaatgcc cgacgtcctc cacaccttcc ggcgcgggca
    11401 ccgcgtgatg gtgcaggtgc agagcagctg gttcccgctc gtcgacagga acccgcagac
    11461 gttcgtcccg gtccgcaccg cgggtgagac cgactaccgc ccggccacga tgcgcgtcca
    11521 cgacggcgcg gagagcccct cgcgcgtcgt cctgcgggtg ctgccgccgg agcgaccctg
    11581 acgccggacg gcgccttcag cccggcagat cccagatcgt gatcgagccg ccgacgaggg
    11641 cgactcttcg tccccgtgcc ttcagcgcgc gaacgagctc ggcgacctgc cgcgcggctg
    11701 cctctccctc gaactccctc acgaacggct cgccgagcgt ctccatcatg tgcgagacgg
    11761 cgtagcggcc cggacggatc ggggccgcgg ggtcgagcac gcgtgcccgg cgcgaccagt
    11821 agttcgtcgc gacgccgctg aacgcgacga tcgtcgtctc ttcctcccac ccccgctcgg
    11881 agaggtagag gtggagccgg tagaggtcct gcccccagtc gacgttcgag tcggagaacc
    11941 agcggcgccc gccttcggga cctcccgcca ggacgttgaa ccaggcgatc ggcgagtccc
    12001 cggcggagca gagcgtgagt cccgccgaca ggacgagcgc cccggcgagc gccgcgcgga
    12061 gccgggggcg cgccgcgagc tcgcggccga cgacgacggc gcccgcgaag acgaggaggg
    12121 cccagatcgg gaggagatgc cgcgcgccga tgttgaaggc ggagcgcgtc gaggcaagga
    12181 ggagcgccgc cgaaacggcg agcgcgagga ggaccgcggt cgtcgtcgat ggccctccgg
    12241 taccggagcg acgaagccgc gcgaggccga ggacgaaagc ggtcgccagg agcacgagaa
    12301 ccgccgacgt cgacttgagg aggaacgccg ccgggaagta gagcgggaag cccttctccg
    12361 aaacctcgcc gcggaaccag ttcgccccgc gcccgcgctc gctcaggagg gcgactccct
    12421 tcgccccggc gacgtagtgg ccgatcggcg ggacggcccg cgcgagggcg gcgtagcgct
    12481 cgacctcgtc ggggcgcgcg gggcgtcccg acaggtaccc gcgtacggac gcctcgacga
    12541 gcgccgccgg catcgcgcga aggacgtagg cgtagacgcc gtagacgacg aggccggcca
    12601 ggccgagggc cgcgagcgcg gcgaggccgc gatcgcgcag gcccccccgc ggcgtcggcc
    12661 cgccccctcc ctggccgggc cgaacgagcg acggcgcgag cggcagcagc gcgacgacgg
    12721 ggacgaggag ggccgtggag aacttcgtga cgagcgcgag cccgagcgcc aggccgagcg
    12781 cgagccaggg gccgagcctc gcggtgagag ccgcccgtgc cgcgagcgcg acgacggcga
    12841 gaaacgcgag agcggcgccg acgtcggtgt ggacgtaggc ggcgtgcgcg acgaacgtcg
    12901 ggtcgagggc gacgagcccg gccgcgagaa gcccggccgc gggccccgcg agcggccgca
    12961 ccgcgaggtg cagcgcgacg acgagtatcg cgaggaccca cgggaagggg cgtctccccg
    13021 ccgcgacgag gacgtgcgcc ggagcggcgt tcccgtagag ccaccgcggg tacgccccga
    13081 aaggcgacgc ggcgcagggc gtctcgccgc agggactccg gaggccgagc ggccggagcg
    13141 cgaacgctcc agcgagcttc aggaggggcg ggtgctccgg gttcatccag ctggagccgt
    13201 cctcgagcgc ctcggccccg gagtagaggt ggatcccctc gtccgaggtc ggggagtcgt
    13261 ccttctggtg cgcgtggacg gcggcgaggg agagcgtcac gaggccggcg agcaggagcg
    13321 cctcgaggag gatcccgaag ggccgcggcg accgcgttcc tcctgcttcc gtcatggggg
    13381 cgtccgctcg ccggggaggc cggtttgccc gagggcgatc tgccggaggg agaggggaat
    13441 ctcggcgcgg atgcgctgga ggagggcttt catgcgggcc gcggggcgat tctaggccgc
    13501 ggtcagacgt cgaggctatc cggtgctcgc cggaggggct cggcttcgcg cgccccccgg
    13561 cccccccacc gaccttcagc tcacacgtcc agcgcgtcgg cttcccgcgc ccgctcggtg
    13621 atgaacctgt agcgggcgcc ggcgtccttg cccatcaggt cggcgaaggt ccgttcggcc
    13681 tcgaggacgt cggggacggt caccttgagc gcccgtcggc ggcgcgggtc gagcgtcgtc
    13741 gccttgagct gctccggcat catctcgccg agccccttga accgcatgac ctcgggcttc
    13801 acgttcttcg ggagcttcgc gaagaccgcg tcccgctccg cctcgtcgag agcccagtac
    13861 gtctctttcc ccgcgtcgat ccggtagagc ggtggctggg cgaggtagac gtgcccctcg
    13921 gtgacgagct tcggcaggtg ccgccagaag aaggtcagga ggagcgtcga gatgtggtgc
    13981 ccgtccgcgt cggcgtccat gaggaggaag accttgccgt agcggagctt gccgaggtcg
    14041 aagtcggccc cgctcccgca gccgagcgcc gagacgatgt cggtcagctc cttgttcccg
    14101 gccaccttcg ccgagttcgc ctgctcggcg ttcaggacct ttccgcggag cgggaggatc
    14161 gcctgcgtct ggcggtcgcg cccctgcttg gccgagccgc cggccgagtc cccctcgacg
    14221 atgaagagct ccgactcgga cgggtccgtc gacgagcagt cggcgagctt cccggggagg
    14281 ttgaggcgat gcgagatcgc cgtcttccgc tgcacctgct gcgcggccgc acgggaggcc
    14341 tcgcgggcgc gggtcgccat gacgatccgc ccgacgatct gctcggcgac ggaggggttc
    14401 tcgttgagcc agttctcgac ggcggggcgg accgcgccgt cgacctgcgc ggcgacctcc
    14461 gggttgttca gccgctcctt cgtctgcccc tggaactgcg gctcgaggac gtagaccgag
    14521 agcagggcga cgagcccctc gcggatgtcc tcggccgagg ggttcacgcc cttcggaagg
    14581 agctcgtgcg tcccgagaaa gttccggacc gccttgacga ggcccgcctt gaggccgttc
    14641 tcgtgcgccc cgccggaggg tgtcgggatg ccgttgaccc acgagcggag gtgctcgtcg
    14701 gtcgactcgg tccactggag ggcgagctcg aggcgcgggt cggcctcccg cgcgaggacg
    14761 aatccgttcg cgtgggtcgg cgccttggcc cgctcggcga cgaccttcag gaggtagtcg
    14821 gcgaggccgc cgtcgtgcgc gtactcctcg cggacggccg gcctcttcgt ctcgtcgacg
    14881 aagacgatct tcaggccgcg gtggagatag gtcttcgcct cgagtcggtc ccggatctcg
    14941 tcggcgtcga acgcgagctt cccgaagatc tccgggtcgg ggcgaaagcg gaccgtcgtc
    15001 ccgctcccgc gcaccttttc ggtcgagacc ttcgtcacct tcgaggtggc cttgccctgc
    15061 gcgtagctct ggcgccactc gtgaccgtcg cggcggacgt tcacgaccat ctccgaggag
    15121 agggcgcaga cgacggacga gccgacgccg tggaggccgc cggagtggac gtagttcccc
    15181 tgctcgaact tcccgcccgc gtggagcgtc gtcaggatca gctcgagcgc cgacttcttg
    15241 tagcgcgggt gaacgtcgac cgggatgccg cgcccgttgt cgtagacctc gacctcgcgt
    15301 ccgtccgccc cgaggcgcac ttcgatgcgc gaggcgtagc cgttgatcac ctcgtcgacg
    15361 gcgttgtcga cgatctccca gacgaggtgg tggaggccgc gggaatcgac gcccccgatg
    15421 tacatgcccg gacgcttccg gacgggctcg agcccctcga ggacggtgat gtcggcggct
    15481 gtgtagctgg aggaagccat gacgggcggc cgacggtaca gaccggagga cggcgcgtca
    15541 aatgacgcgg gcgaggccac gaccgaccgc agttccgtct aacctccgcc tcgtgccgcc
    15601 cgggaccgac ctccttccgc gcctcttcgg gctctgggtc cacctcctgg agaggaccgg
    15661 cggcgccgcc cgcctcgcgg cgctccccgt cccgatcgcg ccggccgcac ggcccctcgc
    15721 cgctctcctc gcgggagccg cggcggtcct ccttctccag gccgctctgg cctccgggcg
    15781 ggacgtggcc gtccgggcgt tccgcgcggc ggccctcctg gccgccctcg ccctccccct
    15841 cgccgccctc cgcctcgtcc ccgcctcccg cgggccggcg atcggcggcg ctgcactcct
    15901 cctcgtcgcg ttctcctcga tcgcccatcg cgccctcgcc tcgcgccgcg ccgaggggac
    15961 cgcatcccgc ctcctcgccc gggcccgcct cctcctcgag gggaccgggc tggcgctctc
    16021 cgccctctct ctcgggctcc tcctcgcggg gcgggcgctt ccgctccgcc tcgccttctg
    16081 gtcgctgttc ctcctccgcc tctcgatcgc cgacctgatc gacccgtcgc gcctcgccgc
    16141 gcggacgggg ctggcgggct cggccgcgcg cgacgcccgg agcgcgctcg ggaaggtccg
    16201 gcggcctccg cgtcccctcc ggcggctgcg ccgcgcgctc gccgcggcgg cgaagggaag
    16261 cctcctcctc ctctggctcg cgctcccgct cgcggccgcc ctcgccccgg gcgaggtcgc
    16321 cgccggggcg tggccccggt gggctctcca gctccggtgg tacccgcctc tcgctctcgc
    16381 gctcacggcg ctcctgctgc tcgggcaggc ggggcgttcg ttccgcggcg gtcgccctct
    16441 cgacgccgcc cggggcgccg ccgtcggggt cggcacggcg gcgtggctgg cgctcgcgta
    16501 tcgcgacggc gcgttcgcgg cctggcggag cgcgctcccg ggcctcgtcc tcgccgaggc
    16561 ggtcgcgggg tttctcctcg gcgcagccgc ccgcggccgc gcgcgctagc gagccgcctc
    16621 agccattcgc gaggcgggcc gcgagctccg cggcgatccg ccgcccctcg tccccatcga
    16681 gccgcccgct cggccagtcg atcccgaggc cgccaccggc ggaaggcttc gggatgacgt
    16741 ggaagtgaac gtgcggcacc gcctggtggg cggcggcgcc gttgttctgc aggacgttga
    16801 agtcgcgggt cccggtgacg gcgacgacgg cccgggcgat gcgcggcagg acgcgtccga
    16861 tcgcggcggc ggactcttcc gaaagcgcgt cgagcgtcgg tgcgggctcc ttcgggacga
    16921 cgaggacgtg ccccggcgcg agcggcgcga tgtcgaggaa cgcgaagacc cgttcgtcct
    16981 cgtagacgcg gtgacacggg atctctcccc ggatgatctt cgtgaagacg gtctcgctca
    17041 cggcctgcct ccgcccttct ccccggccgc gtccttcgcg gccgtgtcga cgagccagat
    17101 gttcccggtc gtctcggcga gctcgtagta gatggcttcg cccgtcggcg accactccgg
    17161 gtagcggatg tagctgttca gcctctcgtt cgcggtcacg atccggaccg tcccgccgtc
    17221 acgcgtgacc cacgcgacgt tccagacccc gtcccgcagg gcggcgaacg cgactttcga
    17281 gccgtcgggc gagaacgaat agggccagct ctgcccgctt ccgctcgtca gctgcacggg
    17341 cgtcccgccg ctcaccggaa cggtgaagac gtggatgtcc ccctcgcgct tggcctcgaa
    17401 cgcgagcgtc cgcccgtcgg gcgaccagca cgggaacgcg gcgagctccc ggtcgaacgt
    17461 gacctggcgg ggcgtccccg cgggaagctc cgcgacccag acgttcaggg tcgtcccgcc
    17521 ccggttcgag tggaaggcga ccttcgtccc gtcgggcgac aggcgcatcg cgtcgtgacc
    17581 cgggccgaag tcggcgagga gctcctcgcg cccggtcgtc aggtcgagcg tgaagagcgc
    17641 ccggtgcccg cggcgctgcg acatgaacgc gagccgcgcg tctcccggga accagaacgg
    17701 gtagtcgtcg tcgccggggt cggtcgtccg ctggacggcg ttgccgccgt cggcgtcgac
    17761 gatccagacg tcctggttcg ccccggtccg ccagcggctg agggcgatcc gcttcccgtc
    17821 cctcgcgagg gcgggccgcg agtttcggcc cgtctcccgc gtgaccgcct gctcgttcgc
    17881 cgtccgctct ccccggccgt cgacggggac cttccagacg ttgctcgcca tcgagagccc
    17941 cgcccacgcg agcctcgtcc cgtcgcgcga gatcgccagc tgcttgacga acgcggtccc
    18001 cgcgctgccg agcgtggcga cctcctccgg ggcacccgtc gtccgccccg tcgccgggtc
    18061 gatcctccgg cggtagaggc cggcggcgaa gtcgaccgac cggcacgggt aggtgatcga
    18121 cccgccgtcc gcgctccaga ccgggtcgta gcactgcccg ttcccctcgc tcacctggac
    18181 gaggtcctcg ccgtccactc cgacgaccca gatctccgac gaggtgcggt cgtaggccgc
    18241 gaacgcgatc cgcttcccgt ccggcgacca cgaggcggcg ccgtggccac cgctcgggcg
    18301 accgacgcgc gtgagtggcc tcgggggcgc accgtcgagc ggggcgatcc agaggacgga
    18361 cggcgggagc gcgccgagcg agttcgcggc gaagtcgacg atcgagcccg actcgaggac
    18421 gagcgccgat ccgtccgggg cgaaggaggg acgggagccg aacgtcgtca gctggcgcgg
    18481 cacgcctccg agcgcgggaa tcagccagac gccgccgacg gacttcgcgt ggtacgcgat
    18541 cgtctcgcca tcgggcgacc aggcgggcga gaggttctga cgcccgtccg tcgtgagcgc
    18601 gatctcccgg ccgcccggcg cgagcgggag acgatagagc tcgaaccggc cggtccggtc
    18661 cgaggcgtag acgatcgtcc gcccgtcggg cgcgaacgcc gggaagacgt cgagacccgt
    18721 ggaggtcgtc agctgcacgg ggcggtagag cgcggcggcg ggcgcctcct ctccccgtcc
    18781 gcggaacagg tagagaccgg ctgccaccag cgcgaggagc gcggcgccga tcccggcggc
    18841 gaggcggacg cccggccgct tcgcgaacgg cggcgccggg ggcgggatcg tggagagacg
    18901 cccggtcgag gggcgcttcg gcgccgcttc gtcctcccag cccccctcct cgagcacggc
    18961 gccgatctcg gccgcgaggt cgcgcatgga ggggaagcgc tcgtccggct ctttcgcgag
    19021 cgcccggtcc gcgatcgcgg cgaacccgcg gaggactccg gtctcgaagc ccgggatcgg
    19081 cgggaccggg aggtggagga tcgcgtacat gacgtcgacc gccgtctccc cgccgaaggg
    19141 ggccttgccc gcgaggagct cgtagaagac ggcgccgagg gagaagacgt cgctccgtgc
    19201 gtccgccttc ttcccgctcg cctgctcggg cgacatgtac tgggccgtcc cgacgacgaa
    19261 gccgtcggtc gtcagcgagg gctggctctt ccgctcgtcg gacgggaccg actggacctt
    19321 cgcgaggccg aagtcgagga ctcggatccg tccctgccgg tcgaccatga ggttggaggg
    19381 cttgatgtcg cggtgcagga acccggcgtc gtggacggcg gcgagcccct cggcggcctg
    19441 ggcgatccag cgcgccgcct tcggtgcggg gaccgctccg ccgaggagga tcccttcgag
    19501 cgtctcccct tcgacgaact cctggacgag gtacgggacc ccgtcgaact cgcccacgtc
    19561 gaagacctgg acgaggttcg ggtgcgacag ggaggccatg acccgcccct cgttcccgaa
    19621 gcggatccgg cgatcgggcg tgccgacgag gtcgagcggg agggtcttga gggcgacgat
    19681 gcggccgagc gaagtgtcct cggcccggta gacctctccc atcgccccgc ttccgaccga
    19741 agcgaggatc cgataccgcc cgaccagcgt gccgggggga atggaggaag acggttccgc
    19801 agtcatcggc cggctcgacg ctctcctctg cccctcctcg gggcgcaaac cgcagtctca
    19861 cacgatctta ctctgggcgg cggcgcccgc gaccgctcct atactcgacc gccgaggccc
    19921 cgatgcgata cgtcgacagt ccgatgcccg cggggcgacg tctcctcgtc cccctgctcc
    19981 tcgccgcgct cgtcgccacg ccgtcgtgca gaaggctccg ctccgccacg tccgaggccg
    20041 agcctcggcc ggtgacggcg cgcggagacc tcgcggccga cgagcgggcg accatcgacc
    20101 tcttccggca gtcgagcccc tcggtcgtct tcatcacgac cctctcccgc cgccggaccg
    20161 gcctcttccg gatcgccgag atcccgcggg gggaaggctc cggcttcgtc tgggacgcgg
    20221 acggccacgt cgtcacgaac ttccacgtca tccagggagc ctcctcggcc cgagtcacgc
    20281 tcgccgacgg ctccacctgg cccgcctcct tcgtcggcgg ggcgcccgac cgggacctcg
    20341 cggtcctccg gatctttccc accgggcgca cgctccgtcc gatcctgatc gggacgtcga
    20401 aggacctcct cgtcgggcag aaggtcttcg cgatcggcaa cccgttcggc ctcgaccagt
    20461 ccctctcgac gggcatcgtc agcgccctcg gccggtcgat cgagtcggtc acggggcgcc
    20521 agatcgaggg ggtcatccag accgacgccg ccatcaaccc cgggaactcc ggaggcccgc
    20581 tcctcgacag cgccggacgc ctcatcggcg tgaacacggc aatcgcctcg acgaccggct
    20641 cgagcgccgg gatcggcttc gcggtcccgg tcgacaccgt gaaccaggtc gtcccgcagc
    20701 tcatccagca cggccgcgtc atccgcccgc gcctcggcgt cacgctcgcc gacgacgccg
    20761 tcgcggcccg gctcggcgtc gagggcgcgc tcatcctctc cgtcgagccc gacaccggag
    20821 ccgcggaggc cggcctccgc gggacgacgc gcgacgaaga cggctcgctc ctcctcggcg
    20881 acgtcgtcac ccgcgccggc gagcgccccg tccgttcggc ggacgacctg atcgcggcgc
    20941 tcgaggagaa gaagccgggg gagacgctgc cgctcctcgt cctccgggcc ggggagaccg
    21001 tccgcgtcgc cgtctcgctc tcggccgcgc cctaggccgc ggccggcggg cgcgggagcg
    21061 ccgccgggag gcggatctcg aagaccgagc cctcgtccgg gacgctctcc accgtcacgc
    21121 gccctcccat caggaggcag agctcgcgcg tgatcgacag gccgaggccg ctccctccga
    21181 acctctgggc ggtcgccgcg tccgcctggg cgaacggctc gaagaggcgc gccgccttct
    21241 ccggcgtgat cccgatcccg tcgtcacgga cgcggaagga ggcccagggg cgccccgcct
    21301 cctcgacgag cgccacgtcg acctccaccc gtccgtcctg cgtgaacttg caggcgttcg
    21361 agaggaggtt gaagaggcac tgccgcacct tcatcgcgtc cgacgtcatc acgaacgccg
    21421 cctcgggcga gaaccgcacg acgaggctgt tccggttcct ctccaggagc ggccggacga
    21481 tggcgaccgt ctcctcgacg agaggtcgga ccaggaaggt ctccacgtcg aggtccagcc
    21541 tcccggcctc gatccgggcc aggtcgagga ccgagttgat cagctccagc tggtggagcg
    21601 cggcggagcg gatcttctcg aggtcccgcc cgagagcctg gcgcccctct tcctgcgcct
    21661 cctccgagag catctcggag tagccgatga tcgcgttcag cggggtccgc agctcgtgcg
    21721 agacgttcgc gaggaaacgg ctcttcgtcc ggctcgcctc ctccgcctcc gccgtcgcct
    21781 tctccgccac ctcgcggtgc cgctcggcct cggcacgcgc ctcctcggcc cggacgagcg
    21841 cctcctccgc gcgcgtcttc tcctccgaga ggctccgcgt ccgctccgcg acgagggcga
    21901 ggagctcgga ctcgcgggcg cgcagactcg cgatcctcca tttcaggcct ccccacgtcg
    21961 cccccgccgc ggcgccgagc agccccaccc agaaccacca tgcctcgcgc gcccgggcct
    22021 tcttccggaa cgagaacgtc gccccccgcc cgagccacgc cccgccggcg tcggccgccg
    22081 agacgcggaa gacgtacggg ccgcttggga ggttcgtgta gtacgcgacg cgacgcgtcc
    22141 cgacttccag cggctcgtcg tcgaggccgt cgaggtagat ccggaatcgg acccggtcgg
    22201 gcgccacgag cgtcagcgcc gtgtagtgga tctcgacccg ctcggcccgc ggggggagga
    22261 cgagcggaac gcgcaggtcc accggccgcc cgtcgaccag gacctcctcg atcgcgaccg
    22321 gcggcgtccg ccccgaggcc gggatgcggg ccggatcgag gaccgccagt ccccggaacg
    22381 tcgggaacca gagccgcccg tcggcggcga cgaagcccgc gggctgctgc cctcccgccg
    22441 cgctcgcgct gcgcatcccg tccgacgcgc cgagggcgaa cgggcggagc atcgccttcc
    22501 ggccgtcggc cacggcctcg agctcctccc gctcgacgcg aaagaccccc ttgttgcacg
    22561 tcatccagag ggagcccttc ccgtcgtcca ggatcacctg aaccaggtcg tcgaagagcc
    22621 cttcgcgcgt cgtcacggac gtgaccgtct cgccgcggat ccggttcagc ccgccgcccg
    22681 acgttccgat ccagaccgca ccgcccgcgt cgggaaagac cgtgaggacc cggttgctcg
    22741 tcaggccttc ccgcgtcgtg aacgcacgga accggccttc cccgtagcgg accgccccgc
    22801 ccccggacga accgatccag agggccccgt ccggggcctc ggcgagcgcc acgacgcggt
    22861 cggtcggcag gccgtcccgc gcgaccgtga agacgcggag cgacccgtct ggggcgacgc
    22921 gggtcagccc gccgccgatc gtcccgatcc agacgttccc ctcccggtcc tcgaggaagg
    22981 ccctcacgtc gtgatgcggc agcccctgcg ccggcccgag cggccggaac cggccccgct
    23041 cgaagaccgc ggcccccgcg aggttcgtcc cgacccagag gcggccgcgc cggtcttcga
    23101 ggagcgccga gacgtgatcg ctcgggaggc cgtcggcctc gcggtaggcc gtgacgcgcc
    23161 cctccttcag ccggttcagc ccgcctccgg cggtcgcgat ccagaccgac ccgtcgcgcc
    23221 ccgggcagac cgcgcggacg ttgtcgctcg tcagcccctc gcgcgtcgtg taggtcagga
    23281 agagcccgtc gcgcaggcgg ttcagcccgc ccacccacgt cccgatccag acgctccctt
    23341 cctcgtcgct cgtgagggac cagacctggt cgttcgagag cccctctcgc gtcccgatca
    23401 cctccgggac cccgttgcga agccggccga cgccggcgcc ccacgtgccg aaccagagcg
    23461 tccctcccgc gtcgacgtgg accgcggaga cctggtcggc cgggaagccg tcggcttcac
    23521 ggtagcggac gaccgcgtcg cccttcaggt acgtgagccc gccccccgag gtcgcgaccc
    23581 agacgcctcc cccggcgtcg ggcgcgaggc cgcggacgag ccccgtgccg agcgtcgcgg
    23641 cgtcccagac cttcgtgacc cggccgccct tcacgaccgc cgcgccggcg ccggtcgtgc
    23701 cgacccagag tgcgccgtcg gcatcgaacg cgatcgcccg gacctgctcg agcggcagcc
    23761 cctgcgcccg gccgatccgc tccgctcccc gcccgtcgac aacacgctgg gctccgatcc
    23821 cgtctccggc cacccaggcg tctcccgccc ggtcgaacgc gatcgaccag acgatcccgc
    23881 ccgcgagccc ctcggccgtc ccgatcgccg agaacgtccc gtcgcggcgg cgacagagga
    23941 cgccgccgcc gttcgtgccg gcccagatcg tcccgtcggg cccctccgcg agcgagatga
    24001 cggagttgtg cttcagctcc ggagtgttcc gccggtcgaa cgtcgtgaag cgggcgccgt
    24061 cgaaccggac gagcccctcc tgcgtgccga gccagaggta gccgtcgcgt ccccggagga
    24121 ccgcctgtac cgtgttctgc ggcaggccgt ccccctcctg ccagacgtcg aggccgtact
    24181 gcgacaggcg cttgcccggg tcgagcgccg gagcggcgct cgaggcgagc aggcagacgg
    24241 cgaggagggc cgtcctgtag cgcagcatcc ccggatcata ggcgcgcgcc ccgccgtcgg
    24301 ccggccgacg gagcttgacc ttcccgctcc gggtccgcat gttccggccg tgaggcgcga
    24361 ggcgaagggc ttccgggtca tcgagacgac gggcgtcccg gtgaaggcgt ggacgctcgg
    24421 cgtcccgtgc gaccccgccg ccgagaagca gctcgcgaac gccgcctcgc tcccgatcgt
    24481 ctaccgctgg gtcgccgcga tgcccgacgt ccacgtcggc atcggcgcca ccgtcggcgc
    24541 ggtgatcccg accgtcgaag cgatcatccc ggccgccgtc ggcgtcgacc tcggctgcgg
    24601 catggcggct tcgctcacct cgctcacggc gaacgacctg ccaacgaacc tcgcgcccct
    24661 gcgggacgcg atcgagaagg ctgtcccgca cggtcggacc gattccggcg gacgaagcga
    24721 ccgcggcgcc tggcacgacc cgcccgcgcg gatcggcgag gcctggcggc tcctcgagcc
    24781 gggctggcgg gccatcctcg atgcccggcc cgagctcgat cgcggcaacc accccgcccg
    24841 gcacctcggg acgctcggca ccggaaacca cttcatcgag ctctgcctcg acgaggccgg
    24901 ccgcgtctgg ctcctcctcc acagcggctc gcgcggcgtc gggaaccgga tcgggaccga
    24961 ctacatcgag gtcgcccgcg aggagatgcg cgtccaccag cggaaccttc ccgaccgcga
    25021 cctcgcgtgg ctcaccgagg ggacggagag cttcgagaac tacgtcgccg ccgtctcgtg
    25081 ggcccaggag tacgcgcgga cgaaccgcga cctgatgctc gtcgcgatcg aggaggcgct
    25141 cgcctcggtc ccgtcgctcc cgccgttccg ctcgcgcctc ctcgccgtct catgtcacca
    25201 caactacgtc cgccgcgagc ggcactacgg gcgcgaggtc tgggtcacgc gcaagggggc
    25261 cgtccgcgcc ggcaagggcg agctcgggat cattccgggg agcatggggg cgcgctcctt
    25321 catcgtccgc ggcctcggca accccgagag cttcgagtcg tgcagccacg gcgccgggcg
    25381 cgccttctca cggaacgagg cgcgccgccg cttcaccctc gccgaccacg ccgaggcgac
    25441 cgccggcgtc gagtgccgga aggacgaggc cgtcctcgac gagacgcccg ccgcctacaa
    25501 gccgatcgag gcggtgatga acgcgcagaa ggacctcgtc gagatcctcc acacgctcaa
    25561 gcaggtggtc tgcgtgaagg gatagccctg cctcaggtcg gagctcgtcc ggcccccgtt
    25621 ctctccccgc gcccatctgt ctctccgacc ccccgccgaa ggcgcgagca cgacgaaggg
    25681 cggtgggtgg ggggcacggg gagggttcac cggcaccgat ccaggaacgc gcggagcttc
    25741 gggaggaact cctcgggatg ctcgagcggg gccatgtggg cgccggggag cgtcagaagc
    25801 tcgacgtagg gggcgttccc cgccgcctcg accatcgcct cggaggcgga cggcggcgtg
    25861 agcgggtcgt cctctccgac agcggcgagg aacgggacgg cgagcgtccc gagaacgtcg
    25921 aagcggtcgg cccgctccgc catgccgcgc tggtcggcgg cggccgtcgc cggcggcgtc
    25981 gcgaggaaca tcgtccggat cagctgctcg agcgcgcctc cgcgcgacga cggggcgagg
    26041 aactgcggga gcatcgccga aaggagcggc tccatcccgt ccttctcgat cgcctccgcc
    26101 gtcgcggctc gcttcgcctt ccccgcctcg tcgtccgcgt tcgcgcgcga gttgcagagg
    26161 acgaggccac gcaggcgcgg gcggagctcc ggtccgaggc gcgcgatcag ctcgagggcg
    26221 acgtacccgc ccatcgacat ccccatgacg acgaacggct cgtcgggccg gaggagcgcc
    26281 gccccgtcct tcgaaaggag cggcatcccg tggaccttct ccgccgacag acccgacttc
    26341 ccgcgcccgc gaaagtcggg cgccgacacg gcgaccccct cgccgagctg gccgctcgcg
    26401 gccgcggccg agacgaggcc ccagagccgg ccgtcgaggg ggaacgcatg gagggcgagg
    26461 aggcggagcg cggcgggcgt ctcggggcgc atcattcggc gaggatagcc cgcgctgggg
    26521 cgcggccgtc ccgacatcac gatcagcccg gaggtcccgt gcccgcttct cgtgcccacg
    26581 ccgcccctgt cgcgctcctc ctcgtcgcgc tcctcgcgcc ccgtccgctc ctcggcgacg
    26641 acctcttcgc ccagctcggc gacgccgccc gcgacggtcg cctcgaggcc gaggcgcggc
    26701 ttcgcgaagg ctccgtgccg gtgatggccg gctactcggt caccgtgaag ggcgcccccg
    26761 cgacgcgcct cggcgtcgcc gtccgcgagg ggaagctgga atctctccgc tacgtcgctc
    26821 ccgacgacac cttcctcctc gtcgggcggg gcctccgccc cgacgtcgtc ctcacgcgga
    26881 tcgagaccga cccctccggc acgatcaccg ccctcgactt ccacggacga ggcctcggca
    26941 ggctcgtcgt cggcctcttc cggccgctcg tgaggaggaa ggtccggcag atgaagctcc
    27001 acaccgagct cgccgagctc ttccgcggga ac
//
LOCUS       DAOONL010000041        26799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1628_length_26799_cov_17.577214, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000041 DAOONL010000000
VERSION     DAOONL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26799
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_26799_cov_17.577214"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..459
                     /locus_tag="PLP50_08115"
     CDS             52..459
                     /locus_tag="PLP50_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51552.1"
                     /translation="MRQSRLRRSAGALVAVLAATLLLPVFALARATAPPAPSHGCHCP
                     VKMACCEAGLCHGDMDTEPSADPSWSGCRDEAPHRAIVPPPSAAFDSALLLGAAPAAP
                     EAGTPTSFVTPAGGDAPRPGPPTPPPRSSLAAC"
     gene            543..2831
                     /locus_tag="PLP50_08120"
     CDS             543..2831
                     /locus_tag="PLP50_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51553.1"
                     /translation="MKLQTCVLVALLSARGLASEPAPATLSGSILAADGSGLPGARVT
                     VVPSPSGSGPAGTVASGERGLYLVRDLRPGTYELVVELPGFETGLRKDVSLSAGERRE
                     VDVTLRLAGVRERLTVVGPSPRDAIEAAEIRESSARDTGEALEKLPGVWKLRKGGIAN
                     EVVVRGSQSRDLNVLVDGERIYGACPNHMDPPAFHVDFAQVERVEVAKGPFDVRNQGS
                     LGGVVNIVTRRPEPGLHATANAAAGAWGYVNPSVTASWAGASFSALAGYSFRRSEAFA
                     DGEGRRFTERTNYRADSVEDEAFRAGTAWARVAFVPAEGHSVSLSYTRQETSHTYYPY
                     LQMDAVFDDADRFGLTWEATPSAVVTSVRLSAYYSQVDHFMTDEYRTSSTTAARGWSM
                     GTDAFSRTLGGRLEATAWGVTIGVEAYRRYWDTTTYLAGMQYKPQASLPGAASDVVGV
                     FAERSFRLGDVVTLDAGLRWDRASASIDGAKASTDLAWAYHGTRATSRADSLPGGSLR
                     ARWRAAEGLEVSLGAGTAARIPEPQERWFSLKRMGTDWVGNPDLAPSRNAALDLNASF
                     RAGRLYVSASAFVNDVSDYVVVSDAKKVNMVPGVMNPAARTYENVDARLAGGEATAAF
                     TLTERLFFSGAVSYVRGTQDPRPEVGILSKDLAEMPPVTGRASLRWDDGRFWGEAEGV
                     FAAAQSNVDTDLAEGTTPGWGIANLRAGFTIGSVGLTLGVENLFGRLYAEHLSYQRDP
                     FRSGVKVPEPGRNVFVNLSYRY"
     gene            2964..4922
                     /locus_tag="PLP50_08125"
     CDS             2964..4922
                     /locus_tag="PLP50_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA51554.1"
                     /translation="MPIRARIPSLSDWSAQVKCQTGCPVSTDAGRYVQLIAEGRDEDA
                     FLVARAPNPFASVCGRVCAAPCEDACRRGAIDAPVSIRALKRFVTERYGVESVRPQTQ
                     DRLFGELVGEGNRYAGHLPVVPFPAHSPAGGKVRVAVIGAGPAGLSAAHDLALLGYDV
                     TVFEAAGEPGGMMRFGIPEYRLPRTLIRAEIDRILALGVTLRLGARLSPSFGLAELRR
                     TGFEAVFLSVGVSQGRELDAPGVELDGVVKAVDYLLNVNRGFRMDLGRRVVVVGGGFV
                     AFDAARTALRVSKEGELEALEEAAGAADARAKEALDSARAALRGGAAEVTIVSLENFD
                     EMPVLRSTQGHEEFEEAKREGVAFLPRRGPKRFVGTSRLEAVELRKVLSVFDESGRFS
                     PRYDDADVVTLPADACVLAIGQKPDLSFLTPEDGVALTPGGTVKVDPATLATSAPGVY
                     AGGDVAFGPRNLIEAVANGKKAARSIHEHLSARGASAGLALETHLDVEKLFTPTYRMI
                     AGFELADRVTPPTIDVGRRTGIAEVETGYDEAEARRQGARCLVCHVQTIYDPEKCVLC
                     SRCVDVCPEYCLAIVPFEELELDEAERSLLEERANGNGLPLSAMVKDDDACIRCGLCA
                     VRCPTDAMTMERFTITERLVPKTSEVSR"
     gene            4919..5467
                     /locus_tag="PLP50_08130"
     CDS             4919..5467
                     /locus_tag="PLP50_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HPA51555.1"
                     /translation="MTTKTDPPAGAAAPPAEPNSRRDFLMKAGLGAGVVALVGQAGAS
                     LRSLVPNVSYDAPTTVKLGAPTDFPDGMKFLPDERLFVFREGKTFHAISAVCTHLGCT
                     VRAEALSKVEEANVEGKPLRLTHRFQCPCHGSKYSGDGTNETGPAPKPLAWYQLSVAA
                     DDGQLVVDLAQEVGHDFRLTVA"
     gene            5484..6827
                     /locus_tag="PLP50_08135"
     CDS             5484..6827
                     /locus_tag="PLP50_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HPA51556.1"
                     /translation="MSAKTEPPASSSAPGVHPKPFWSFRPRSDREAGDAVVSNFLLHW
                     FPAKTYKPSLDWSYSFWLGTASAALFFLLVLSGLPLLLLYVPSVERAYGSVKDIEFVV
                     TFGSWIRAVHRIAAHLMVAAVFLHLVRVFLTGAYKNGTGRGQRREWNWVLGVVMIVLT
                     LFLSFTGYLLPWDQLAYWAVTVGTNIASSVPFVGPQMRELLIGGRTIDQATLIRFYVL
                     HVIFLPAGLGALLAYHMWRIRKDGGLAKADRWTPTQEKTEASPAKSKTYSLLGVARGQ
                     APAIRAVTLEARDTTVNSVNDLVRRISIVVLGTFAVVSILACFVPSPLEEPANPMITP
                     NPAKAPWYFLWLQEIVTDTTLKIGSFTINGAFLGGVILPTLLLGLLTVWPWLDRSPAG
                     AAGVWFHSSRRRQNLVFLLVVLLVLAFTIVGTFLRGPYWNFYWPWEAWPELPTRI"
     gene            6830..8050
                     /locus_tag="PLP50_08140"
     CDS             6830..8050
                     /locus_tag="PLP50_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPA51557.1"
                     /translation="MEQRTSSPYPGKDRPVLAVIGVLLVVSTVVFAVTDHRKDWRWYQ
                     AEFRKMVAEKYGAEKAASVPAGMKQIWVKDLGRADRCITCHQGILWKGFETAEHPWKT
                     HPAEPLKIHPVEDYGCTACHGGQGWAVESEKAHGPIHFWEEPVLGKAMGEAYSLQGGD
                     KGALMQMNCNVCHRYDRETKGADLINLGKKLVNEKGCRACHVINGRGGTIGPDLTFVG
                     DKAAEQYDMSRLGGQKTAFAWHVAHLKNPKELVPDTVMPSFGFGTKESQALAALVLSW
                     RRAPVPATYRPGVPRTDPQTPEEIAEEERMKHGPGAWFVSTGCFVCHSVSSLGVKSAA
                     QVGPDLSTAVEDTQSRFGKTVDDFIRNPTGTMSVVLSRQIVLTDAEKEVAIQKLREAF
                     AEHQKQKAAGPEAR"
     gene            8161..10029
                     /gene="nosZ"
                     /locus_tag="PLP50_08145"
     CDS             8161..10029
                     /gene="nosZ"
                     /locus_tag="PLP50_08145"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HPA51558.1"
                     /translation="MTEKKKSVLTWGVLGAIVVAGVLAGRCAPSGPKGKKAPAAASDI
                     AVAAQKTYVAPGDLDEYYFFASGGHSGQMYVYGVPSMRHLSTIPVFTPYPATGYGFDD
                     ESKAMLNGLTWGDAHHPAPSETAGDYDGRWLFINDMNGRIARIDLRDFKTKQILGPVA
                     NVSGNHSGAYVTPNTEYAMMGSRFSAPVPKTNAVAIEKYASEYKGILAAIKIDPKSGE
                     MSLGWQIVTPPFNWDLGDAGKKVSDGWMFWTCYNSERATGKLEVTASQADRDYIAAVD
                     WRAAEKAIAEGKGEMLGGVKVLDPKKVPGLMYLMPCGKSPHGVDVSPDGKYVIGSGKL
                     QGVTTAFNFEKVLTAIRNKDFTGDEDGIPVLKYESIKDAEVEVGLGPLHTQFDGDGYA
                     YTSLFVDSAIAKWKLGTWEVVDKIPMSYSIGHLCAAEGDTVSPDGKYLVGLNKLSHGR
                     HLNVGPSQPESSQLVDISEEKMKLLYDAFTEPEPHYAVMIKADKLKPIEVYPKEENKH
                     PLAIWDVKDAGVTRKGTEVLAKVVLVRSTITPELIEVNQGDTVKVALTNIEQTTDELH
                     GWGLLDYNINIVVDPGETKVVTFKADKPGVFAYYCTNFCSALHQEMQGYLVVKPKG"
     gene            10153..10779
                     /locus_tag="PLP50_08150"
     CDS             10153..10779
                     /locus_tag="PLP50_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51559.1"
                     /translation="MRSLLERSNRLLEQPIGLKPRLLLVAATICLLGAYLFPLWNLTM
                     FAPQYQDGLRLDIYSYKFEAGNDGQDIKEINVLNHYIGMRDLEPEDFTEFKWIPFVVG
                     IMGLLFLRAAVFGHMAHVLDVLVLYTWFGLFSLWSFGYKMYRYGHDLDPKAAMNVEPF
                     MPPLFGGKQLANFEVFSYPQAGSYTLALAMVFLVLAFYFGWRESRKPA"
     gene            10781..12163
                     /gene="nosD"
                     /locus_tag="PLP50_08155"
     CDS             10781..12163
                     /gene="nosD"
                     /locus_tag="PLP50_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HPA51560.1"
                     /translation="MRSPAAAALLLALALPARAEEMAPVLPGALEGRPAPRDASPLQK
                     LVDAAPPGATVEVPTGTYEGDLVLDHPVRLVGRGRPLLLGSRSGSVVRVRAPNVTVEG
                     FDIDGREGGDLGKDSAGVHTSAPGTVVRSCRIRGALFGVYVREANDAAVEECEIRGIP
                     GKAPGEKGSGIHVWNTVGFRFVKNTIVDVRDGFYIQSSSQGLVAGNRASDLRYGIHYM
                     FSDDNVFEDNVFENGAAGSALMYSKRITFRRNQFLRNRGFASVGLLFKTCDDVVAEGN
                     LLADNARGVFFEDSYRNVFRGNVVASSDTAIVLFAGCGQNRITGNSFVGNLTPLTLVG
                     KTTDTVFDGNYWSENREPDLDGDGRSDRPYRLGNVFDHFRGNLLAADLFAQSFAAGAI
                     GVAEETFPVLRQQMAMDNAPLARPPLLPDVPRAKPVARAAHVPAVAASAATFLLGTAL
                     LVLGRRLPAA"
     gene            12177..13070
                     /locus_tag="PLP50_08160"
     CDS             12177..13070
                     /locus_tag="PLP50_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51561.1"
                     /translation="MIRFDAFGKSYGTQVAVRDVSLEVGAGEVVALLGPNGSGKTTCL
                     KAVAGLIHATSGRVLVDGRPASEPAARRGLSFLPQKVAFPDALTGREVVEFYRRLRGV
                     PAPRTAEVLRFACLNGAAARAVSTYSGGMVQRLGLAVAALPDSPLLLLDEPTAALDPD
                     GLSAFYALVEGRRSEGRTILFTSHHLGDVERLADRFAVLVEGRLVALLSAAELAHRLA
                     DRGVLRLRLDRSPAGVVDSIRAACPGASATEQELLLPGPASARPAYLEAVRATGATVV
                     ALSSDEGRLDALYTELVGGPR"
     gene            13067..13519
                     /locus_tag="PLP50_08165"
     CDS             13067..13519
                     /locus_tag="PLP50_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HPA51562.1"
                     /translation="MKPALRIAAAVSILLLAACGGGAEGPVPVDTKNDSCAWCRMSVS
                     DLRFAAQLTAPGHEPKLFDDAGCLRDWLKEPRESAPWTAWLSDHRTKEWVKATDAVVV
                     REPAIQTPMDSGLVAWASAASREQDPAGKGGTPVPIADVIGPFTPGVK"
     gene            13516..14352
                     /locus_tag="PLP50_08170"
     CDS             13516..14352
                     /locus_tag="PLP50_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPA51563.1"
                     /translation="MSDSSFVLCARQELLLAIRSRWTQIFAVVFAGLALAVAVAGYAL
                     SGGHGVQDFARTSASLVQLVLLLVPLSSLLIGVLSLAPERGAAELLFSQPVSRTSILL
                     GQATGLWAALTAAQAIGFGAAGTVVFSHRGSDGLGGFALLFAGSSLLTAVFLGLAALL
                     AAGAIGRRRTRALALALVVWFVAVVLLDVVALGVASVLRSGTASRVLILSVIVNPVGA
                     LRTGALLALQGTTAFGSASLAFLRFTGGTTWAFVLLSGSLAAWTVIPFALASRRLSRT
                     DL"
     gene            14426..16996
                     /locus_tag="PLP50_08175"
     CDS             14426..16996
                     /locus_tag="PLP50_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51564.1"
                     /translation="MKYAPLAAAAAAALLLFSNPAVASGAAYRSVLYPVAGNEGSSGD
                     REAGANGAFWIPSSAYDNPTGGWIPIVHRLDPSGDVTRWELDSTARSFFPVAVRWRTA
                     DSRAIVLVPLGAEQRALVYALDPATGAATRTEVPFLARSLRLDPQDGAPWLVGRDTVA
                     RLSGGTLQTWTIPGVVEAAGRFDAGGRLWLATEGGVLLSMTRDGALASWGPGSSPGTL
                     LTVDEGGNELWGVDGARDRLVRFRPSTGERTAWPQVRPVSNWAGLAVSGGTVALAASY
                     GPHVLSGAIADLAGGTTTVATSAPADSGTPANTPAPAVAVTTTRSDSTPSPTARTLYA
                     PAEDGRGLFTTSGEAYRALLWAGAGTFLVATDGVELWTPLAAGAMFTTRQVLPVAVEV
                     RPNDPTRNFFTEVAVSNLDAERDVVLTLTTTSATHTLSIDLPAGTTRVFPNVVQSFRD
                     LGGAALPPGETVAGTLEARFRNGSGGLSARVFTRFPDESTTGLGFTSLDAAREALVFR
                     KALNGLKNTAGFRTNVAVANLCGLEESCSTLDVSADFFDDATGTAVGTATLQVPPGQW
                     RQLDAPLAGFAGATGETFSVVFVPFSVGATAYDAYATVVSNANQDAAFVRATPSERAS
                     NLTLPVITDAGGIDTRFTSELALTNTTGAPAVADVTFTSAKSGNVVSETLELAAGLGV
                     LWPNAVGHFRSISPASVQADDYGPVRFAFRDFASGFASVRTTASNGTGLGFTAIDPYV
                     ERAKRTKRIVGLVENARFRTNLAVVHAGATGADPSAQITLRVTVRDASGAVVGTPLEK
                     TLGPGQLHQWTKILSDPLGRSGEGYTATIERVDGFDPFDAYVTVIDNASTDPVFVRAE
                     "
     gene            complement(17006..>17265)
                     /locus_tag="PLP50_08180"
     CDS             complement(17006..>17265)
                     /locus_tag="PLP50_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HPA51565.1"
                     /translation="LAAPPAWHDEARVVARIDADEILAKGDHPLGAVQGAAASLPKGG
                     ELLLVSSFLPAPLVDAMRKKGFETASFRAEGGRVATLLRLP"
     assembly_gap    17266..17365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17366..17688)
                     /locus_tag="PLP50_08185"
     CDS             complement(<17366..17688)
                     /locus_tag="PLP50_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPA51566.1"
                     /translation="MSGLPITPETKVGELLEAYPELEETLVAIAPAFSKLKNPVLRRT
                     VARVATLAQAARVGGVEVRTLVGTLRQAAGLAGEPTAAEAAAAGEILAAPPAWHDEAR
                     VVARI"
     gene            complement(17685..18923)
                     /locus_tag="PLP50_08190"
     CDS             complement(17685..18923)
                     /locus_tag="PLP50_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPA51567.1"
                     /translation="MSELINNREKRIEELKGIIRHLHAGASPDEVRGQLREIVRETDA
                     TEIAAMEQSLIADGMPVEEVRSMCDLHSQVLREFVVPAPAVGDVPPGHPVDTFRRENE
                     AILAAVARLRESLAALGDTGTSGARARVRAATNELFDVEKHYRRKEELLFPFLEKHGI
                     VGPSKVMWAKDDEARGLLKALAEALSVEEASTEEWRLVVETVGEPAAAAIAEMVLKEE
                     LVLLPLSLGTLSADEWGLVHAESPRVGWCLVPPREGWLPPLPSFPSDALSLPAGKAVA
                     LPSGHLTLEQLQGIFEALPVDLTFVDADDRVAFFSEGPDRVFSRTRAILGRKVQHCHP
                     PKSVDVVERILDDFRAGTQSVAEFWLELHGRFVHVRYFAVRDAKGVYAGCLEVTQDAT
                     RIRALAGERRLLQYESAVPA"
     gene            complement(19090..19761)
                     /locus_tag="PLP50_08195"
     CDS             complement(19090..19761)
                     /locus_tag="PLP50_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPA51568.1"
                     /translation="MSTALLERIALFRGLTPAETKALALVCRVQVCGRGETVFVEGTR
                     ATEMSFVALGTVKIVKSTPTRSVILRIVGAGGPVGIVAAWEGRPYPGTAIAVEPSTVV
                     HVPEREFFALSDRHPEILRQLLQLMMRRQVQLGQRVSEMTGSVESRIAQALLDLLASA
                     AKADGARAVVPLSLTRQEIADLAGTTVETAIRIMSRWGKEGLVLTEEDGFVIPDVTRL
                     RALVG"
     gene            19836..21566
                     /locus_tag="PLP50_08200"
     CDS             19836..21566
                     /locus_tag="PLP50_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51569.1"
                     /translation="MKDSARFFSYFSRRPGAYAAGFGTMALSAALFLAMPRIVRHVLE
                     SLETDPSPRRIAFACGAILLLGAADAVLFFFTRRILIGASRDVEFEMREDLFAHLLRL
                     PPRWHRKSRVGDVMSRAVNDLSAVRMMIGPGIMQAANTVVVGSIALTLMVFASPSLTL
                     VALAVLPFVVVATQVMGKVTHRRFTAIQEFFSEISASAQENFNGARLVRAFARETTEE
                     ARFDAKNRELRRRNLRLARLNALYFPLLQALVGSGFAVILWAGGRQILAGELTVARYV
                     EFNLYLMELVWPAIALGWVVNLWQRGTASWNRMAELWAAEPLPEESGDGPLPAGPLEV
                     KGLTFGYDGGPPVLRDVAFTVEPGTTVAVVGRTGSGKSTFLQLLLRLEEPPFGSIRLG
                     GRDLREVPLPLLRRTLVAVPQETFLFSDTLAANVAMGRPGATREEVERAALEAGLGPD
                     LETFPKGLDTIVGERGITLSGGQKQRTALARALLADSPILVLDDAFSAVDTETEERIL
                     EAVKARSGTRTIFLVSHRLSTIQHADFVVVLEKGRVVETGTHEELLRAGGLYAELAER
                     QRLSEEVEAA"
     gene            21569..23422
                     /locus_tag="PLP50_08205"
     CDS             21569..23422
                     /locus_tag="PLP50_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51570.1"
                     /translation="MAGHLPEDEAQGRGFDRGLLRRLFTLALPYRGYAGGALLVLFLE
                     SAAQLAGPLLTGAAIDLVFTPKGEAAGGAAFVARLLASLGVPVSGPRALDAIAGLYLL
                     SVLANFGLMFLMVEWTSRMGQGVMYDLRTRIFGHLQRSDVALYDKTPVGRLMTRLTTD
                     VDALNELFTSGFVSLLGDLAVLFGIVAVLYALDPTLATITLAVLVPMALVTNWFRKGA
                     RDTYRKVRVRIAKVNSFLQEHLSGIAVVQLFRREQPARTAFREVNDDHRQANLESIFY
                     YAVFYPALDFLSAVGMAAILWWGGGEVIRGTMTIGAVVTFLQYSKRFYRPLMDLSEKY
                     NILQAAMASSERIFELLDESPKVAAPPSPRPVPRPLRGEIDFDAVRFSYAPGEDVLKG
                     VSFRAAPGETIALVGATGSGKSTIVNLLLRFYDVTGGAVRVDGHDVRAFTPTEFRDGF
                     GIVLQDTFLFTGTIAENVAFFDPAMPRETIERACRDVGLGPLLERLPDGLDTRLTERG
                     GGLSVGEKQLVSFARALARDPRILILDEATSSVDPETEAVIERAVDRLLSGRTSVVVA
                     HRLSTIVRADRILVLSHGEVRESGTHAELLAKGGLYARLYALQLKDREAAA"
     gene            complement(23447..25903)
                     /locus_tag="PLP50_08210"
     CDS             complement(23447..25903)
                     /locus_tag="PLP50_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPA51571.1"
                     /translation="MCGIVSVCHGREVPGLGHEAGELLKRLEYRGYDSTGAAFVRADR
                     SVRLLKKVGAPSRVVPELGIDREAGQRFVGQVRWATYGAVTDANAQPHHVRCRVELVG
                     AHNGNISSTDTLRPRLSAGGHDVVSDNDGEALTHLAEDAFAGGLADPAQILAAGRAAL
                     AAAGLDVAVPDRALLLIEAARRADAGVEGSYAAAYADPASEGVVAVKSGSSLYAGIGA
                     DETGSFVLVSSDLTSVLSRTRMLVPLAEGEGIWFTESAYAVFPLAGPIGFASPRPRRS
                     KLNVRDTGLRPPFAYFMDQEIASSSENLEEIVRTYFRTPETEGLFAAFEDRVDLCKAL
                     VEKLLKLYGATDEAGLERGFRDLLGEPLLTELVARVRAHRELLVSYGPFVSDEKSLLA
                     DGVRLVPDAAEAAALLDLVLVWKKRRRVTTLLQELVSAIRTTQKEGGRVFLVASGTSY
                     HAALTAGYFFNVLAGVAVFPCNPGTFRSLYLNSLKPEDLLLGISQSGETKDLVDVFQD
                     VRARVPALRRVSLVNNENSRIPQELSEFYLPILCGPEIAVAATKSFLNQVAVLYVVAA
                     SFSLNERRIVEKLSAARALVTETLRRCEADVDEAAERLYLEPSLHILGTGLIGLAREG
                     ALKIREVVLNHAEGYDAAEFKHGPNTILGKNTLFSIHDLAGVLEAWEERRGAGPFPGG
                     LEALRLHPELVERHFSNYPLLFVCPPEERDVRITVSQIHTHKIRGADILVVCEPSDDL
                     ARAAAGRPAGDGRYWSKTIEVPPTGDPALFVFAASVVLQRLAFRMSVKKMEYLDRLRV
                     ADHGVHPDAPKNVSKSITVD"
     gene            26146..26478
                     /gene="ytxJ"
                     /locus_tag="PLP50_08215"
     CDS             26146..26478
                     /gene="ytxJ"
                     /locus_tag="PLP50_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HPA51572.1"
                     /translation="MQPVRDAEALERAFGEPWALVFKHSTRCPVSSAAWREVAAFRKA
                     HPEAVVYVVHVPEQRSLSNALAARTGIRHESPQAILLENGRVAWHDSHEGVTAEALGR
                     LLPAAVVR"
BASE COUNT         3687 a        10067 c         8983 g         3962 t
ORIGIN      
        1 atgaccggcg tcatactcgg accgcccgat cggacctaga agggacccgt catgagacag
       61 agcaggttgc ggcgctcggc cggggcgctc gtcgccgtcc tcgcggcgac gcttctgctg
      121 cctgtcttcg ctctcgcgag ggccaccgct cctcccgcgc cctcgcacgg ctgtcactgc
      181 cccgtgaaga tggcctgctg cgaagccggc ctctgccacg gggacatgga tacggagccc
      241 tccgccgatc cctcgtggtc ggggtgccgc gacgaggccc cccaccgggc gatcgttcct
      301 cccccgtcgg ccgcgttcga cagcgccctc ctcctcgggg cggctccggc cgcgcccgag
      361 gccggcacgc cgacctcgtt cgtgacgccg gcgggcggcg acgcgccccg ccccggtccg
      421 ccgaccccgc ctccccgctc ctccctcgcc gcctgctgac ggtccggggc ccggcccctc
      481 cgcccggcgc tccgcgcggc acggccgcgt ggagtccgtc gatgcaccgc gatggagcat
      541 tcatgaagct gcagacctgc gtcctcgtcg ctctcctctc ggcccggggc ctcgcgtcgg
      601 agcccgcccc ggcgacactc tcgggctcga tcctcgccgc ggacggaagc ggccttcccg
      661 gggcccgcgt caccgtcgtc ccctcgcctt cggggtccgg ccctgccgga accgtcgcgt
      721 ccggcgaacg ggggctctac ctcgtccggg acctgcggcc cgggacctac gagctcgtcg
      781 tcgagcttcc gggcttcgag acgggacttc ggaaggacgt ctcgctctcc gccggcgagc
      841 ggcgcgaggt cgacgtgacg cttcgcctcg ccggggtgcg cgaacgcctc acggtcgtcg
      901 gcccttcgcc ccgcgacgcg atcgaggccg ccgagatccg cgagagctcg gcgcgtgaca
      961 cgggcgaggc gctcgagaag ctcccgggcg tctggaagct ccggaaggga ggcatcgcga
     1021 acgaggtcgt cgtgcgcggc tcccagagcc gcgacctgaa cgtcctcgtc gacggcgagc
     1081 ggatctacgg ggcctgcccg aaccacatgg acccgcccgc cttccacgtc gacttcgccc
     1141 aggtcgagcg ggtcgaggtg gccaaggggc cgttcgacgt gcggaaccag gggagcctcg
     1201 gcggcgtcgt gaacatcgtg acgcgccggc ccgagcccgg gcttcacgcc accgcgaacg
     1261 ctgccgcggg ggcgtggggc tacgtcaacc cgtcggtcac cgcctcgtgg gccggcgcgt
     1321 cgttctcggc gctcgccgga tactccttcc ggcgatcgga agcgttcgcg gacggcgagg
     1381 gccggcgctt caccgagcgg acgaactacc gcgccgactc cgtcgaggac gaggcgttcc
     1441 gggcggggac cgcctgggcc cgggtcgcat tcgtcccggc cgagggccac tcggtctccc
     1501 tctcgtacac gcggcaggag acctctcaca cctactaccc gtacctgcag atggacgcgg
     1561 tcttcgacga cgcggaccgc ttcggcctga cctgggaggc gacgccctct gccgtcgtca
     1621 cgtcggtccg gctctccgcc tactactcgc aggtcgacca tttcatgacc gacgagtacc
     1681 ggacctcgtc gacgaccgcg gcgcgcggct ggtcgatggg gaccgacgcc ttctcgagga
     1741 cgctcggcgg acggctggag gcgaccgcgt ggggcgtgac gatcggcgtc gaggcgtacc
     1801 ggaggtactg ggacacgacg acgtacctcg ccgggatgca atacaagccg caggcctccc
     1861 ttcccggggc ggcgagcgac gtcgtcggcg tcttcgccga gcggtcgttc cgcctcggcg
     1921 acgtcgtgac gctcgacgcc ggcctgcgct gggaccgcgc ttcggcatcg atcgacggcg
     1981 cgaaggcctc gaccgacctc gcctgggcct atcacggcac gcgcgccacg agccgcgccg
     2041 actcccttcc cggcgggagc ctccgcgccc ggtggcgggc cgcggaaggc ctcgaggtct
     2101 ccctcggcgc cgggacggcc gcgcggattc ccgagccgca ggagcgctgg ttctccctga
     2161 agcggatggg gaccgactgg gtcgggaacc cggacctcgc cccttcgcgg aacgccgctc
     2221 tcgacctcaa cgcttcgttt cgcgccgggc gcctctacgt ctcggcgagc gcgttcgtca
     2281 acgacgtctc cgactacgtc gtcgtctccg atgcaaagaa ggtgaacatg gtcccgggcg
     2341 tcatgaaccc ggccgcccgc acctacgaga acgtcgacgc ccggctcgcc ggcggcgaag
     2401 cgaccgcggc cttcaccctg acggaacgcc tcttcttctc gggcgccgtg tcgtacgtcc
     2461 gcgggacgca ggacccgcgc cccgaggtcg ggatcctctc gaaggacctc gcggagatgc
     2521 cccccgtcac cggacgggct tcgctccgct gggacgacgg ccgcttctgg ggcgaggccg
     2581 aaggcgtctt cgcggccgcg caatcgaacg tcgacacgga ccttgccgag gggacgacgc
     2641 ccggctgggg catcgcgaac ctgcgagccg gtttcactat cgggagcgtc ggcctgaccc
     2701 tcggcgtcga gaacctgttc ggtcgtctct acgccgagca cctctcctac cagcgggacc
     2761 cgttccggtc gggcgtgaag gtcccggagc ctggccggaa cgtcttcgtc aacctctcgt
     2821 accgctactg atcggtcccc tccagggagc cggggccgag gatcggcggc cccggctccc
     2881 cccttttcac cctcgcccca tgacctgcgt cataccgcgc ccgcgcccgt gcgccgatcc
     2941 tcctcggcac agggagattc gacatgccga tccgggctcg tatcccgtcc ttgtcggatt
     3001 ggagcgctca ggtcaaatgc cagacgggct gtccggtttc gaccgacgcc gggcgctacg
     3061 tccagctcat agcagagggg cgggacgagg acgcgtttct cgtcgcccgg gcgccgaacc
     3121 ccttcgcttc ggtctgcggc cgggtctgcg ccgcgccgtg cgaggacgcc tgccgccgtg
     3181 gcgccatcga cgcccccgtc tccatccggg cgctcaagcg gttcgtgacg gagcgctacg
     3241 gagtcgagtc ggtccgcccc cagacgcagg accgcctctt cggcgagctc gtcggcgagg
     3301 ggaaccggta cgcgggccac cttcccgtcg tgcccttccc ggcgcactct ccggccggtg
     3361 gcaaggtccg cgtcgcggtc atcggcgcgg gaccggccgg cctctcggcc gcgcacgacc
     3421 tggcgctcct cggctacgac gtgaccgtct tcgaggcggc cggcgagccg ggcggcatga
     3481 tgcgcttcgg catccccgag taccgcctgc cgcggactct cataagggcg gagatcgacc
     3541 ggatcctcgc gctcggcgtc acgctccgac tcggcgcgcg gctctccccc tcgttcggcc
     3601 tcgccgagct ccgccgtacc gggttcgaag cggtcttcct ctccgtcggc gtctcgcagg
     3661 ggcgcgagct ggacgccccg ggcgtcgagc tggacggcgt cgtgaaggcg gtcgactacc
     3721 tcctgaacgt gaaccggggc ttccggatgg atctcggacg gcgggtcgtc gtcgtcggag
     3781 gcggcttcgt cgcgttcgac gcggcccgga ccgcgctgcg cgtctccaag gagggggagc
     3841 tcgaggcgct cgaggaggcg gcgggcgccg cggacgcgcg cgccaaggag gccctcgact
     3901 cggcccgcgc ggccctgcgc ggaggggcgg cggaggtgac gatcgtctcg ctcgagaact
     3961 tcgacgagat gcccgtcctg cgctccacgc aggggcacga ggagttcgag gaggcgaaac
     4021 gggagggggt cgccttcctc ccgcggcggg gcccgaagcg cttcgtcggc acgtcgcgcc
     4081 tcgaggccgt cgagctgcgg aaggtcctct ccgtcttcga cgagagcggc cgcttctccc
     4141 cgcgctacga cgacgccgac gtcgtcacgc tcccggccga cgcctgcgtc ctggcgatcg
     4201 gccagaagcc cgacctctcc ttcctgaccc ccgaggacgg cgtcgcgctc acgcccggcg
     4261 gcacggtgaa ggtcgacccg gccacgctcg ccacgtcggc gccgggcgtc tacgccggcg
     4321 gcgacgtggc gttcggcccg cggaacctca tcgaggcggt cgcgaacggc aagaaagccg
     4381 cccgctcgat ccacgagcac ctctccgccc gcggcgcctc ggcgggtctc gcgctcgaga
     4441 cgcacctcga tgtcgagaag ctcttcacgc cgacataccg gatgatcgcg ggcttcgagc
     4501 tcgcggaccg ggtcacgccg ccgacgatcg acgtcggccg gcggactggg atcgcggagg
     4561 tcgagaccgg ctacgacgag gccgaggcgc gccgccaggg ggcgcgctgc ctcgtctgcc
     4621 acgtccagac gatctacgac ccggagaagt gcgtcctctg cagccgctgc gtcgacgtct
     4681 gccccgagta ctgcctcgcc atcgtcccgt ttgaggagct cgagctcgac gaggcggagc
     4741 ggtcgctcct cgaggagcgc gcgaacggga acggcctccc cctctcggcg atggtcaagg
     4801 acgacgacgc gtgcatccgc tgcggcctct gcgccgtccg ctgccccacc gacgcgatga
     4861 cgatggagcg attcacgatc acggaacgtc tcgttccgaa gacgagcgag gtttctcgat
     4921 gacgacaaag acggaccccc cggccggcgc cgccgcgcct cccgccgagc cgaacagccg
     4981 acgcgacttc ctgatgaagg ccggcctcgg cgccggcgtc gtcgccctcg tcgggcaggc
     5041 gggcgcctcc ctgcgctcgc tcgtcccgaa cgtgtcgtac gacgcgccga cgacggtgaa
     5101 gctcggcgcc ccgacggact tccccgacgg gatgaagttc cttcccgacg agaggctctt
     5161 cgtcttccgc gaggggaaga ccttccacgc gatctcggcc gtctgcacgc acctcggctg
     5221 caccgtgagg gccgaggcgc tctcgaaggt cgaggaggcg aacgtcgagg ggaagcccct
     5281 ccgcctcacg caccgcttcc agtgcccctg ccacgggtcg aagtactccg gcgacggcac
     5341 gaacgagacg gggccggcgc cgaagccgct cgcctggtac cagctctccg tcgccgccga
     5401 cgacgggcag ctcgtcgtcg acctcgcgca ggaagtcggg cacgacttcc gcctgacggt
     5461 cgcctgagcg gagcgggacg aacttgagcg cgaagaccga gccccccgcc tcttcctcgg
     5521 cccccggagt ccacccgaag cccttctgga gcttccgccc ccgctccgac cgcgaggcgg
     5581 gagacgccgt cgtctccaac ttcctcctcc actggttccc ggccaagacc tacaagccgt
     5641 cgctcgactg gagctactcg ttctggctcg gcacggcctc ggccgccctc ttcttcctcc
     5701 tcgtcctctc gggcctccct ctcctcctcc tctacgttcc ctcggtcgag cgggcctacg
     5761 gatcggtgaa ggacatcgag ttcgtcgtca cgttcggctc ctggatccgc gccgtccacc
     5821 ggatcgcggc ccacctcatg gtggcggccg tcttcctcca cctcgtccgc gtcttcctca
     5881 ccggcgccta caagaacggc acggggcgcg ggcagcggcg cgagtggaac tgggtcctcg
     5941 gcgtcgtcat gatcgtcctg acgctgttcc tctccttcac gggctacctc ctcccgtggg
     6001 accagctcgc ctactgggcc gtcacggtcg ggacgaacat cgcgagctcg gtgccgttcg
     6061 tcggccccca gatgcgcgag ctcctgatcg gcgggcggac gatcgaccag gcgacgctca
     6121 tccgcttcta cgtcctgcac gtcatcttcc tgccggccgg cctcggcgct ctcctcgcgt
     6181 accacatgtg gcgcatccgc aaggacggcg ggctcgcgaa ggccgaccgc tggacgccga
     6241 cgcaggagaa gaccgaggcc tcgcccgcga agtcgaagac ttattcgctc ctcggcgtcg
     6301 cccgcggcca ggccccggcg atccgcgccg tgactctcga ggcgcgggac acgacggtga
     6361 actccgtcaa cgacctcgtc cggcggatct cgatcgtcgt cctcgggacg ttcgccgtcg
     6421 tgagcatcct ggcctgcttc gtcccgtcgc ccctcgagga gccggccaac ccgatgatca
     6481 cgccgaaccc ggcgaaggcg ccgtggtact tcctctggct ccaggagatc gtcaccgaca
     6541 cgacgctgaa gatcgggtcg ttcacgatca acggcgcgtt cctcggcggc gtcatcctgc
     6601 cgacgctcct cctcggcctc ctcaccgtct ggccgtggct cgacaggagc ccggccggcg
     6661 cggccggagt ctggttccac tcctcgagga ggaggcagaa cctcgtcttc ctcctcgtcg
     6721 tcctcctcgt cctcgcgttc acgatcgtcg ggaccttcct ccggggtccc tactggaact
     6781 tctactggcc ctgggaagcc tggcccgagc tgccgacgag gatctgacca tggagcagcg
     6841 cacgagctcg ccctaccccg gcaaggaccg ccccgtcctc gcggtcatcg gggtcctcct
     6901 cgtcgtctcg acggtcgtct tcgccgtcac cgaccaccgg aaggactggc gctggtacca
     6961 ggcggagttc cggaagatgg tagcggagaa gtacggcgcc gagaaggccg cgtccgtgcc
     7021 ggccggtatg aagcagatct gggtgaagga cctcggccgc gcggaccggt gcatcacctg
     7081 ccaccagggg atcctctgga agggcttcga gacggccgag cacccctgga agacccatcc
     7141 cgccgagccg ctgaagatcc accccgtcga ggactacggc tgcaccgcct gtcacggcgg
     7201 gcagggctgg gccgtcgaga gcgagaaggc ccacgggccg atccacttct gggaggagcc
     7261 cgtcctcggg aaggcgatgg gcgaggccta ctcgctccag gggggcgaca agggcgccct
     7321 gatgcagatg aactgcaacg tctgccaccg ctacgaccgg gagacgaagg gggcggacct
     7381 catcaacctc ggcaagaagc tcgtcaacga gaagggctgc cgggcctgcc acgtcatcaa
     7441 cgggcgcgga ggcaccatcg gccccgacct gacgttcgtc ggcgacaagg ccgccgagca
     7501 gtacgacatg agccgcctcg gcggccagaa gaccgcgttc gcctggcacg tcgcccacct
     7561 gaagaacccg aaggagctcg tccccgacac cgtcatgccg agcttcgggt tcgggacgaa
     7621 ggagtcccag gccctcgcgg cactcgtcct ctcctggcgc cgcgcccccg tcccggcgac
     7681 gtaccgaccg ggcgtcccgc ggaccgaccc gcagacgccc gaggagatcg ccgaggagga
     7741 gcgaatgaag cacgggccgg gcgcctggtt cgtctcgacc ggctgcttcg tctgtcactc
     7801 ggtctcctcg ctcggggtga agagcgccgc gcaggtcggg cccgacctct cgacggccgt
     7861 cgaggacacg cagagccgct tcgggaagac cgtggacgac ttcatccgga acccgaccgg
     7921 gacgatgtcg gtcgtcctgt cgcgccagat cgtcctgacc gacgccgaga aggaggtcgc
     7981 gatccagaag ctgcgcgaag ccttcgccga gcaccagaag cagaaagccg ccggccccga
     8041 ggcccgctga tcgactccgg ggggcgcgcg ccgatcccgt gcgccccccg ctcaccgcac
     8101 ctcaaccgat tcgcccccgg cctcgaaccg ggggcctcac agggagaagg aggggaccag
     8161 atgacggaaa aaaagaagag cgtcctgacg tggggcgtcc tcggagcgat cgtcgtggcc
     8221 ggagtcctgg cggggcgctg cgccccgtcc gggccgaaag ggaagaaggc cccggcggcc
     8281 gcctcggaca tcgccgtcgc ggcgcagaag acctacgtcg ccccgggcga cctcgacgag
     8341 tactacttct tcgcctcggg cggccactcg ggccagatgt acgtctacgg cgtgccgtcg
     8401 atgcggcacc tctccacgat cccggtcttc acgccctacc cggccacggg ctacggcttc
     8461 gacgacgagt cgaaggcgat gctgaacggc ctgacgtggg gcgacgcgca ccaccccgcg
     8521 ccctccgaga cggccggcga ctacgacggc cgctggctct tcatcaacga catgaacggc
     8581 cggatcgcgc ggatcgacct gcgcgacttc aagacgaagc agatcctcgg gcccgtcgcg
     8641 aacgtctccg ggaaccactc cggcgcctac gtcacgccga acaccgagta cgcgatgatg
     8701 ggctcccgtt tctcggcgcc tgtcccgaag acgaacgccg tcgcgatcga aaagtacgcc
     8761 tccgagtaca aggggatcct ggccgccatc aagatcgacc cgaagagcgg cgagatgtcg
     8821 ctcgggtggc agatcgtgac gccgccgttc aactgggacc tcggtgacgc tggcaagaag
     8881 gtctccgacg ggtggatgtt ctggacctgc tacaactccg agcgcgccac cggcaagctc
     8941 gaggtgaccg cctcgcaggc cgaccgcgac tacatcgccg ccgtcgactg gagggccgcc
     9001 gagaaggcga tcgccgaggg gaagggcgag atgctgggcg gcgtcaaggt cctcgacccg
     9061 aagaaggtcc ccggcctcat gtacctgatg ccgtgcggca agtcccccca cggggtcgac
     9121 gtctcccccg acggcaagta cgtcatcggc tcggggaagc tccagggcgt cacgacggcc
     9181 ttcaacttcg agaaggtcct gacggcgatc cggaacaagg acttcaccgg cgacgaggac
     9241 ggcatccccg tcctgaagta cgagtcgatc aaggacgccg aggtcgaggt cggcctcggc
     9301 ccgctccaca cgcagttcga cggcgacggc tacgcctaca cctcgctctt cgtcgactcg
     9361 gcgatcgcga agtggaagct cggcacctgg gaggtcgtcg acaagatccc gatgtcctac
     9421 tccatcggcc acctctgcgc ggccgagggc gacacggtga gcccggacgg gaagtacctc
     9481 gtcggcctca acaagctctc gcacggccgg cacctgaacg tcgggccgtc gcagcccgag
     9541 tcgtcccagc tcgtcgacat ctccgaggag aagatgaagc tcctctacga cgccttcacc
     9601 gagcccgagc ctcactacgc cgtgatgatc aaggccgaca agctcaagcc gatcgaggtc
     9661 taccccaagg aggagaacaa gcacccgctc gcgatctggg acgtcaagga cgccggcgtc
     9721 acccgcaagg ggaccgaagt cctcgcgaag gtcgtcctcg tgaggtccac gattaccccc
     9781 gagctcatcg aggtgaacca gggcgacacg gtcaaggtcg cgctgacgaa catcgagcag
     9841 acgacggacg agctccacgg gtggggcctc ctcgactaca acatcaacat cgtcgtcgac
     9901 ccgggcgaga cgaaggtcgt cacgttcaag gccgacaagc cgggcgtctt cgcctactac
     9961 tgcacgaact tctgctccgc tctccaccag gagatgcagg gctacctcgt cgtcaagccg
    10021 aagggctgac ggctctccct ccggacgggg cggcgccgcg gggcgccgtc ccgctcctcg
    10081 ccccttcgtc cgcggccgtc cccgggggtg cggcccggac cgccttccgg acccccgatc
    10141 gagggcaccg acatgcgatc gctcctggaa agaagcaacc gtctcctcga gcagccgatc
    10201 ggcctgaaac cccggctcct cctcgtcgcc gcgacgatct gcctcctcgg ggcgtacctc
    10261 ttcccgctct ggaacctgac gatgttcgcc ccgcagtacc aggacggcct ccgcctcgac
    10321 atctacagct acaagttcga ggcgggaaac gacgggcagg acatcaagga gatcaacgtc
    10381 ctaaaccact acatcggcat gagggacctc gagcccgagg acttcaccga gttcaagtgg
    10441 atcccgttcg tcgtcgggat catggggctc ctcttcctcc gcgccgcggt cttcgggcac
    10501 atggcccacg tgctcgacgt gctcgtcctc tacacctggt tcgggctctt ctcgctctgg
    10561 tccttcggct acaagatgta ccgctacggt cacgacctcg acccgaaggc ggccatgaac
    10621 gtcgagccct tcatgcctcc gctcttcggc ggcaagcagc tcgcgaactt cgaggtcttc
    10681 tcgtatcccc aggccggctc ctacaccctg gctctggcga tggtcttcct cgtcctggcg
    10741 ttctacttcg ggtggcgcga gtcgcggaag cccgcctgac gtgagatccc cggcagccgc
    10801 cgcgcttctc ctcgcgctcg ccctcccggc gcgagccgag gagatggccc cggtcctccc
    10861 gggcgccctc gaggggcgcc ccgcgccgcg cgacgcctcg cctctccaga agctcgtcga
    10921 cgcggcgccc ccgggcgcga cggtcgaggt cccgacggga acgtacgagg gagacctcgt
    10981 cctcgaccac cccgttcgtc tcgtcggccg cggacgcccc ctcctcctcg gctcccggtc
    11041 cgggagcgtc gtccgcgtgc gggccccgaa cgtcaccgtg gaggggttcg acatcgacgg
    11101 acgggagggg ggcgacctcg gcaaggactc cgccggggtc cacacctccg cgccgggaac
    11161 cgtcgtccgc tcctgccgga tccggggggc gctcttcggc gtctacgtcc gcgaggcgaa
    11221 cgacgcggcc gtcgaggagt gcgagatccg gggcatcccg gggaaggcgc cgggcgagaa
    11281 gggctccggc atccacgtct ggaacacggt cggcttccgg ttcgtgaaga acaccatcgt
    11341 ggacgtgcgg gacggcttct acatccagtc ctcgagccag ggtctcgtgg ccggcaatcg
    11401 tgcctcggac ctgcgctacg ggatccacta catgttctcg gacgacaacg tcttcgagga
    11461 caacgtcttc gagaacggcg ccgcggggag cgctctcatg tactcgaagc ggatcacgtt
    11521 ccggaggaac cagttcctcc ggaaccgcgg gttcgcctcg gtcggcctcc tcttcaagac
    11581 gtgcgacgac gtcgtcgccg aggggaacct cctcgccgac aacgcccgcg gcgtcttctt
    11641 cgaggactcc taccggaacg tcttccgcgg gaacgtcgtg gcgtcctccg acacggcgat
    11701 cgtcctcttc gcgggctgcg ggcagaaccg gatcaccggg aactccttcg tcgggaacct
    11761 cacgcccctg acgctcgtcg ggaagacgac cgacaccgtc ttcgacggga actactggtc
    11821 cgagaaccgc gagcccgacc tcgacggcga cgggcgctcc gaccgcccgt accgcctcgg
    11881 gaacgtcttc gaccacttcc gcgggaacct cctcgcggcg gacctcttcg cccagagctt
    11941 cgcggccggg gcgatcggcg tcgcggagga gaccttcccc gtgctccggc agcagatggc
    12001 gatggacaac gctcccctcg cccgcccgcc gctcctcccc gacgtcccgc gggcgaagcc
    12061 ggtggcgcgc gccgcgcacg tccccgccgt cgcggcctcc gccgccacgt tcctcctcgg
    12121 caccgcgctc ctcgtcctgg gccggcgtct cccggcggca tgagagagtg aacgcgatga
    12181 tccgtttcga cgccttcgga aagagctacg gcacccaggt cgccgtccgc gacgtctcgc
    12241 tcgaagtcgg ggccggcgag gtcgtcgcgc tcctgggacc gaacggctcg ggaaagacga
    12301 cctgcctcaa ggcggtcgcg gggctcatcc acgcgacctc gggacgggtc ctcgtggacg
    12361 ggcggccggc ctcggagccc gccgcccgcc gggggctctc cttcctcccg cagaaagtcg
    12421 ccttccccga cgccctgacg gggcgcgagg tcgtggagtt ctacaggcgc ctccggggcg
    12481 tccccgcgcc ccgcacggcc gaggtcctcc gcttcgcgtg cctgaacggg gccgccgcgc
    12541 gcgcggtgtc gacctactcg ggagggatgg tccagcgcct cggcctcgcc gtcgccgcgc
    12601 tccccgactc gccgctcctc ctcctcgacg agccgaccgc cgccctcgac cccgacgggc
    12661 tctccgcctt ctacgccctc gtcgaggggc gccgcagcga ggggaggacg atcctcttca
    12721 cgtcgcacca cctcggcgat gtcgagcgcc tcgcggaccg gttcgccgtc ctcgtggagg
    12781 ggcgcctcgt cgcgctcctc tcggcggcgg agctcgcgca ccggctcgcc gaccggggcg
    12841 tcctgcggct ccgcctcgac cgctcccccg cgggggtggt cgactcgatc cgcgccgcgt
    12901 gcccgggggc ctcggcgacg gagcaggagc tcctcctccc gggtccggcg tccgcccggc
    12961 cggcatacct cgaggccgtg cgcgcgaccg gcgcgacggt cgtcgctctc tcgtccgacg
    13021 aggggcgtct cgacgccctc tacaccgagc tcgtgggagg tccgcgatga agcccgccct
    13081 ccggatcgcc gccgccgtct cgatcctcct cctcgccgcc tgcggaggcg gcgccgaagg
    13141 ccccgttccg gtggacacga agaacgactc ctgcgcctgg tgccggatgt cggtctccga
    13201 cctccgcttc gccgcccagc tgacggcacc cggccacgag ccgaagctct tcgacgacgc
    13261 cggatgcctt cgcgactggc tgaaggagcc gcgggagtcc gcaccgtgga ccgcgtggct
    13321 ctccgaccac cggacgaagg agtgggtgaa ggcgacggac gccgtggtcg tgcgcgagcc
    13381 ggcgatccag acgccgatgg actccgggct cgtcgcctgg gcgagcgccg cctcgcgcga
    13441 gcaggacccg gcgggaaagg gcggcacgcc cgtgccgatc gccgacgtga tcggcccgtt
    13501 cacaccggga gtgaagtgag cgactcttcc ttcgtcctct gcgcccggca ggagctcctc
    13561 ctcgcgatcc gctcccgctg gacccagatc ttcgccgtcg tcttcgccgg cctcgcgctc
    13621 gccgtggccg tcgccggcta cgccctctcg ggcggacacg gagtccagga cttcgcccgg
    13681 acgtcggcct cgctcgtcca gctcgtcctc ctcctcgtcc cgctctcctc gctcctcatc
    13741 ggcgtcctct cgctcgcgcc ggagcggggc gcggcggagc tcctcttctc ccagccggtc
    13801 tcgcgcacgt cgatcctcct cggccaggcc acgggcctct gggccgccct gaccgcggcc
    13861 caggcgatcg gcttcggcgc cgccggaacg gtcgtcttct cccaccgcgg gagcgacggg
    13921 ctcggaggct tcgccctcct cttcgccggc tcctcgctcc tgacggccgt cttcctcggc
    13981 ctcgcggcgc tcctcgcggc cggggcgatc gggcggcgca ggacgcgcgc cctcgcgctc
    14041 gcgctcgtcg tctggttcgt cgcggtcgtc ctgctcgacg tcgtcgccct cggcgtcgcg
    14101 tcggtccttc gatcggggac cgcctctcgg gtcctgatcc tgagcgtgat cgtgaatccc
    14161 gtcggcgccc tgcgcaccgg cgccctcctc gcgctccagg ggacgacggc gttcggctcg
    14221 gcctcgctcg cgttcctccg gttcacgggc gggacgacgt gggccttcgt cctcctctcc
    14281 ggatcgctcg ccgcctggac ggtgattccg ttcgcgctcg cctcgcggag actctcccgg
    14341 accgacctct gatcgccgct tcccgtctgc gcggccgcct cgacggccgc ccggttcccg
    14401 gctatcgtcc cggccggagg cctccatgaa gtacgcaccc ctcgcggccg ccgcggcggc
    14461 ggccctgctt ctgttttcga acccggccgt cgcctcggga gccgcctacc ggagcgtcct
    14521 ctacccggtg gcggggaacg agggctcctc cggcgaccgc gaagcggggg cgaacggcgc
    14581 gttctggatt ccctcctccg cgtacgacaa cccgaccggc ggctggatac cgatcgtcca
    14641 ccggctcgac ccgtccggcg acgtcacgag gtgggagctc gactccacgg cccgctcgtt
    14701 ctttccggtg gccgtgcgct ggcgcaccgc ggacagccgg gcgatcgtcc tcgttccgct
    14761 cggggcggag cagcgggccc tcgtctacgc gctcgacccg gcgaccggcg ccgcaactcg
    14821 aacggaagtc ccgttcctcg cgcggtcgct tcgcctcgac ccgcaggacg gcgcgccctg
    14881 gctcgtcgga agggacacgg tcgcccggct ctccggcggc acgctccaga cctggacgat
    14941 ccccggcgtc gtcgaggccg cgggacggtt cgacgccggc ggccggctct ggctcgcgac
    15001 cgagggcggc gtcctcctgt cgatgacgag ggacggagcc ctcgcgagct ggggacccgg
    15061 ctcgtctccc ggaacgctcc tcaccgtcga cgagggcggc aacgagctct ggggcgtcga
    15121 cggggcgcgg gaccgtctcg tccgcttccg cccgtcgacc ggcgagagga ctgcctggcc
    15181 ccaggtccgg cccgtgtcga actgggcggg cctggcggtc tccggcggga ccgtcgccct
    15241 cgccgcgtct tacggtccgc acgttctctc cggggccatc gccgacctcg cgggcgggac
    15301 gacgaccgtg gcgacctccg ctcccgcgga ctccggaaca ccggcgaaca cgcccgcacc
    15361 cgccgtcgcc gtgacgacga cccgatcgga ctcgacgccg tcgcccacgg cgcggaccct
    15421 ctacgcgccg gccgaggacg ggcgcggcct ctttacgacg agcggagagg cgtaccgcgc
    15481 cctcctctgg gctggcgccg gcacgttcct cgtcgccacg gacggggtcg agctctggac
    15541 cccgctcgcc gcgggcgcga tgttcacgac ccgacaggtc ctgccggtcg ccgtggaagt
    15601 gaggccgaac gacccgacgc ggaacttctt cacggaggtc gccgtctcga atctcgacgc
    15661 ggagagggat gtcgtcctca ctctgacgac cacgagcgcg acgcataccc tctcgatcga
    15721 ccttccggcc gggacgacgc gcgtcttccc gaacgtcgtc cagtcgttcc gcgacctcgg
    15781 cggggccgcg ctcccgccgg gtgagaccgt ggcgggaacg ctcgaggccc gcttccggaa
    15841 cgggtccggc ggcctctcgg cccgcgtctt cacccgcttt cccgacgaat cgacgaccgg
    15901 gctcggtttc acctccctcg acgcggcgcg cgaggcgctc gtcttccgaa aggcgctgaa
    15961 cggcctgaag aacaccgccg gcttccggac gaacgtggcc gtcgcgaacc tctgcggcct
    16021 cgaggagagc tgctccacgc tcgacgtctc ggccgacttc ttcgacgacg ccaccgggac
    16081 cgccgtcgga acggccacgc tccaggtacc gcccgggcag tggagacagc tcgacgcgcc
    16141 gctcgccggc ttcgccggcg ccacgggcga gacgttctcc gtcgtcttcg tcccgttctc
    16201 ggtcggggcc accgcctacg acgcctacgc cacggtcgtc tcgaacgcga accaggacgc
    16261 cgccttcgtc cgggccacgc cttcggagcg cgcgtcgaac ctgactctgc cggtgatcac
    16321 cgacgccggc gggatcgaca cgcgcttcac gtccgagctc gcgctgacga acacgaccgg
    16381 ggcgcccgcc gttgccgacg tgacgttcac gtccgcgaag tccgggaacg tcgtctccga
    16441 gacgctcgag ctcgccgccg gcctcggcgt cctctggccg aacgccgtcg gccatttccg
    16501 ctcgatctcc cccgcctccg ttcaggccga cgactacggc ccggtgcggt tcgcgttccg
    16561 cgatttcgcg agcggattcg cctccgtccg gacgaccgcc tcgaacggga ccggcctcgg
    16621 cttcaccgcg atcgacccgt acgtcgagcg ggcgaagcgg acgaagagga tcgtcggact
    16681 cgtcgagaac gcgcgctttc ggacgaacct cgccgtcgtt cacgccgggg cgacgggcgc
    16741 cgacccgtcg gcgcagatca cgctgcgcgt gaccgtccgc gacgcctcgg gcgccgtcgt
    16801 cgggaccccg ctcgagaaga cgctgggccc gggccagctc catcagtgga cgaagatcct
    16861 ctcggatccc ctcggacggt ccggcgaggg ctacacggcg acgatcgagc gggtcgacgg
    16921 gttcgacccg ttcgacgcct acgtcaccgt catcgacaac gcctcgaccg acccggtctt
    16981 cgtgagggcg gagtagcgcc gcgccctacg gcagccgcag gagcgtcgcg accctcccgc
    17041 cctcggcgcg gaagctcgcc gtctcgaacc ccttcttccg catcgcgtcg acgagcggcg
    17101 cggggaggaa gctcgagacg agcaggagct cgcccccctt cgggagcgac gcggcggctc
    17161 cctgcaccgc gccgagaggg tggtcgccct tcgcgaggat ctcgtcggcg tcgatccggg
    17221 cgacgacccg agcctcgtcg tgccaggccg gcggcgcggc gaggannnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnntcgat ccgggcgacg acccgagcct cgtcgtgcca
    17401 ggccggcggc gcggcgagga tctcgcccgc ggccgcggcc tcggcggccg tcggctcgcc
    17461 cgcgaggccc gccgcctgcc ggagcgtccc gacgagcgtc cgcacctcga cgcccccgac
    17521 ccgcgccgcc tgggcgagcg tcgcgacgcg cgcgacggtc ctccggagga ccgggttctt
    17581 cagcttcgag aaagccggag cgatggcgac gagcgtctcc tcgagctccg ggtaggcctc
    17641 gagaagctcc cccaccttcg tctcgggggt gatcggcagg ccgctcacgc cggcaccgcc
    17701 gactcgtact ggaggagccg ccgctcgccc gcgagggcgc ggatccgggt cgcgtcctgc
    17761 gtgacctcga gacatcccgc gtagacaccc ttcgcgtcac gcacggcgaa gtagcggacg
    17821 tggacgaagc ggccgtggag ctcgagccag aactccgcga cgctctgcgt ccccgcgcgg
    17881 aagtcgtcga ggatgcgctc cacgacgtcg acgctcttcg gcgggtggca gtgctgcacc
    17941 ttccggccga ggatcgcgcg cgtccgcgag aagacccggt cgggtccctc gctgaagaag
    18001 gcgacgcggt cgtcggcgtc gacgaacgtc aggtcgaccg ggagcgcctc gaagatcccc
    18061 tggagctgct cgagcgtcag gtgcccggac ggcagggcga ccgccttccc ggccgggagc
    18121 gacagcgcgt ccgacgggaa gctcggcagc ggcgggagcc acccctcgcg gggcgggacg
    18181 aggcaccagc cgacgcgcgg cgactcggcg tggacgagcc cccactcgtc ggccgagagc
    18241 gtcccgaggg agagcgggag gaggacgagc tcctccttca agaccatctc ggcgatcgcc
    18301 gccgccgccg gctcgccaac cgtctcgacg acgagccgcc actcctccgt cgacgcctcc
    18361 tcgacggata gcgcctcggc gagcgctttc aggaggccgc gcgcctcgtc gtccttcgcc
    18421 cacatcacct tcgacgggcc gacgatcccg tgcttctcga ggaacgggaa gaggagctcc
    18481 tccttcctcc ggtagtgctt ctccacgtcg aagagctcgt tcgtcgccgc ccggacgcgg
    18541 gcgcgggcgc cggacgtccc cgtgtccccg agcgcggcga gggactcgcg gagcctcgcg
    18601 accgccgcga ggatcgcctc gttctcgcgg cggaacgtgt cgaccgggtg accgggcggg
    18661 acgtctccga cggcgggggc cggcacgacg aactcgcgga ggacctgcga gtggaggtca
    18721 cacatcgagc ggacctcctc gaccggcatc ccgtccgcga tgagcgactg ctccatcgcg
    18781 gcgatctccg tcgcgtcggt ctcccggacg atctcgcgca gctgcccgcg cacctcgtcg
    18841 ggcgacgccc cggcgtgtag atgccggatg atccccttca gctcctcgat ccgcttctcg
    18901 cggttgttga tcagctcgct catcgggtcc tcccgttcac gcgacggagg atgacccgca
    18961 ccggcctcag gtcggtatga cgcgcgtcag ggatatcgag atatcgtcgc aggacgagcg
    19021 acgacccggg aggtcgcctg agtccaggaa aggccccccg gaggacgcga ggcgcctccg
    19081 agccccggtt cagccgacaa gcgcccgaag gcgcgtcacg tccgggatga cgaagccgtc
    19141 ctcctccgtc aggacgagcc cctccttccc ccatcggctc atgatccgga tcgccgtctc
    19201 gaccgtcgtc ccggcgaggt cggcgatctc ctggcgggtg agcgagaggg gaacgacggc
    19261 gcgcgcgccg tccgccttcg cggccgaggc gaggaggtcg aggagcgcct gcgcgatccg
    19321 cgactcgacc gagccggtca tctcggagac acgctgcccg agctgcacct gccttcgcat
    19381 catcagctgg aggagctggc ggaggatctc ggggtggcga tcggaaaggg cgaagaactc
    19441 gcgctcgggg acgtggacga ccgtcgaggg ttccacggcg atcgccgtcc ccgggtaggg
    19501 acgcccttcc caggcggcga cgatcccgac cgggcccccg gcgccgacga tccgcaggat
    19561 cacgctccgg gtcggcgtcg acttcacgat cttcacggtc ccgagcgcga cgaaagacat
    19621 ctccgtcgca cgggtcccct cgacgaagac ggtctctccc cgcccgcaga cctggacgcg
    19681 gcagaccagc gcgagggcct tcgtctccgc gggggtgaga ccgcgaaaca gggcgatccg
    19741 ttcgagaagt gcggtcgaca cgatccggaa gcgcgaggat accagtcggg gcggatcggt
    19801 ccgtgccggt tcgggccggg taacatccct tctccttgaa ggactcggct cgcttcttct
    19861 cctacttctc ccgcaggccc ggggcctacg ccgccggctt cgggacgatg gcgctctcgg
    19921 cggccctctt cctcgcgatg ccgcggatcg tgcggcacgt cctggagtcg ctcgagacgg
    19981 acccctcgcc ccgccggatc gccttcgcct gcggcgcgat cctcctcctc ggcgccgccg
    20041 acgccgttct cttcttcttc acccgccgca tcctcatcgg cgcttcgcgc gacgtcgagt
    20101 tcgagatgcg ggaggacctc ttcgcccacc tcctccggct gccgccgcgc tggcaccgga
    20161 agagccgcgt cggcgacgtg atgagccgcg ccgtgaacga cctctcggcc gtccggatga
    20221 tgatcggccc cgggatcatg caggcggcaa acaccgtcgt cgtcgggtcg atcgcgctga
    20281 cgctcatggt cttcgcctcc ccgtcgctca cgctcgtcgc cctcgccgtc ctgccgttcg
    20341 tcgtcgtcgc gacgcaggtc atgggaaagg tcacccaccg gcgcttcacg gcgatccagg
    20401 agttcttcag cgagatctcc gcgagcgcgc aggagaactt caacggggcc cgcctcgtgc
    20461 gggcgttcgc ccgggagacg accgaagagg cgcgcttcga cgcgaagaac cgcgagctgc
    20521 gccgccggaa cctccgcctc gcgcggctga acgcgctcta cttccctctc ctgcaggcgc
    20581 tcgtcgggag cggcttcgcc gtcatcctct gggccggcgg tcggcagatc ctcgcgggcg
    20641 agctgacggt ggcgcggtac gtcgagttca acctctacct gatggagctc gtctggcccg
    20701 cgatcgccct cggctgggtc gtgaacctct ggcagcgcgg gaccgcctcc tggaaccgga
    20761 tggccgagct ctgggcggcc gagcctctcc ccgaggagag cggcgacggg ccgctcccgg
    20821 ccgggccgct cgaggtgaag gggctgacgt tcggctacga cggcgggccg cccgtcctcc
    20881 gcgacgtcgc gttcaccgtc gaaccgggga cgacggtcgc cgtcgtcggc cggaccggct
    20941 cgggaaagtc gacgttcctg cagctcctcc tccgcctcga ggagccgccg ttcgggtcga
    21001 tccgcctcgg cggccgcgac ctgcgcgagg tcccgctccc gctcctgcgc cggacgctcg
    21061 tcgccgtccc gcaggagacg ttcctcttct ccgacacgct cgccgcgaac gtcgcgatgg
    21121 ggcgccccgg ggcgacgcgg gaggaggtcg agcgggccgc gctcgaggcc ggcctcggac
    21181 ccgacctcga gacgttcccg aaggggctcg acaccatcgt cggtgagcgc ggcatcacgc
    21241 tctccggcgg ccagaagcag cggacggccc tcgcccgcgc cctcctcgcc gactcgccga
    21301 tcctcgtcct ggacgacgcc ttctccgcgg tcgacacgga gacggaggag aggatcctcg
    21361 aggccgtgaa ggcccgttcc gggacgcgaa cgatcttcct cgtctcccac cgtctctcca
    21421 cgatccagca cgccgacttc gtcgtcgtcc tcgagaaggg gcgggtggtc gagacgggga
    21481 cccacgagga gctcctccgc gccggcgggc tctacgccga gctcgccgag cgccagcgcc
    21541 tctccgagga agtggaggcc gcgtagcgat ggccggccac ctccccgagg acgaggccca
    21601 gggacgcggc ttcgaccgcg gcctcctgcg gcgcctcttc accctcgccc ttccgtacag
    21661 gggctacgcc ggaggcgccc tcctcgtcct ctttctcgag tcggccgcgc agctcgccgg
    21721 cccgctcctg acgggcgccg cgatcgacct cgtcttcacc ccgaagggag aggccgccgg
    21781 cggggccgcc ttcgtcgcgc gcctcctcgc gtcgctcggc gtaccggtct ccgggccgcg
    21841 cgccctcgac gcgatcgcgg gcctctacct cctctccgtc ctcgcgaact tcggcctcat
    21901 gttcctgatg gtcgaatgga cgtcgcggat gggacagggg gtgatgtacg acctccgaac
    21961 ccggatcttc gggcaccttc agcgctccga cgtcgcgctc tacgacaaga cgcccgtcgg
    22021 ccggctcatg acgcgtctga cgacggacgt cgacgcgctg aacgagctct tcacctccgg
    22081 cttcgtctcc ctcctcgggg acctcgcggt cctcttcggc atcgtcgccg tcctctacgc
    22141 cctcgacccg acgctcgcga cgatcacgct cgccgtcctc gtcccgatgg cgcttgtgac
    22201 gaactggttc cggaaggggg cgcgcgacac ctaccggaag gtccgggtac ggatcgcgaa
    22261 ggtgaactcc ttcctccagg agcacctctc cggcatcgcc gtcgtccagc tcttccggcg
    22321 cgagcagccc gcacggacgg cgttccgcga ggtcaacgac gaccaccgtc aggcgaacct
    22381 cgagtcgatc ttctactacg cggtcttcta ccccgccctc gacttcctct ccgccgtcgg
    22441 catggcggcg atcctctggt ggggcggcgg cgaggtcatc cgcgggacga tgacgatcgg
    22501 cgccgtcgtc acgttcctcc agtactcgaa gcggttctac cggccgctca tggacctctc
    22561 cgagaagtac aacatcctcc aggcggcgat ggcctcctcg gagcggatct tcgagctcct
    22621 cgacgaatct ccgaaggtcg ccgcgccgcc ctcgccccgc cccgtccctc gcccgctccg
    22681 cggcgagatc gacttcgacg ccgtccgctt ctcctacgcg ccgggcgagg acgtcctcaa
    22741 gggggtctcg ttccgcgccg cccccggcga gacgatcgcc ctcgtcgggg cgaccggctc
    22801 ggggaagtcg acgatcgtga acctcctcct ccgcttctac gacgtgaccg gcggcgccgt
    22861 ccgcgtcgac ggtcacgacg tgagggcgtt caccccgacg gagttccgcg acggcttcgg
    22921 gatcgtcctg caggacacgt tcctcttcac cgggacgatc gccgagaacg tggcgttctt
    22981 cgatccggcg atgccgcgcg agaccatcga gcgcgcctgc cgcgacgtcg gcctcgggcc
    23041 gctcctcgaa cgcctccccg acggcctcga cacccggctc acggagcgcg gcggcggcct
    23101 ctccgtcggc gagaagcagc tcgtctcctt cgcccgcgct ctcgcccgcg acccgcggat
    23161 cctgatcctc gacgaggcga cctcgagcgt cgacccggag acggaagccg tcatcgagag
    23221 ggccgtcgac cgcctcctct ccggccgcac ctccgtcgtc gtcgcccacc gcctctcgac
    23281 gatcgtccgg gccgaccgga tcctcgtcct ctcccacggc gaggtgcggg agtcggggac
    23341 gcacgccgag ctcctcgcga agggagggct ctacgcccgc ctctacgcgc ttcagctgaa
    23401 ggaccgggag gcggcagcct gagaggccgc cgccccgcgg acggcatcag tcgaccgtga
    23461 tcgacttcga gacgttcttc ggcgcgtcgg ggtggacgcc gtggtcggcg acccggaggc
    23521 ggtcgaggta ctccatcttc ttcaccgaca tccggaaggc gagacgctgg aggacgacgc
    23581 tcgcggcgaa gacgaagagg gccgggtctc cggtcggcgg gacctcgatc gtcttcgacc
    23641 agtagcgccc gtcccccgcg ggccgccctg ccgccgcccg ggcgaggtcg tcgctcggct
    23701 cgcagacgac gaggatgtcg gcgccccgga tcttgtgcgt gtggatctgg ctcaccgtga
    23761 tcctcacgtc gcgctcctcg ggcgggcaga cgaagaggag cgggtagttc gagaagtgcc
    23821 gttcgacgag ctcggggtgg agccgcaggg cctcgaggcc gccggggaac ggccccgctc
    23881 cacggcgctc ctcccacgcc tcgaggaccc cggcgaggtc gtggatcgag aagagcgtgt
    23941 tcttccccag gatcgtgttc ggcccgtgct tgaactccgc cgcgtcgtac ccctcggcat
    24001 gattcaggac gacctcccgg atcttgagcg ccccctcgcg cgcgaggccg atcaggccgg
    24061 tgccgaggat gtggagactc ggctcgaggt agaggcgctc cgccgcctcg tccacgtccg
    24121 cctcgcagcg gcggagagtc tccgtcacga gggcgcgggc cgccgagagc ttctcgacga
    24181 tccgccgctc gttgagcgag aaggacgccg cgacgacgta gaggacggcg acctggttca
    24241 ggaagctctt cgtcgccgcc accgcgatct ccggcccgca gaggatcggg aggtagaact
    24301 ccgagagctc ctgcgggatc cggctgttct cgttgttcac gagcgagacg cgtcgcaggg
    24361 cggggacgcg ggccctcacg tcctggaaga cgtcgacgag gtctttcgtc tcccccgact
    24421 gcgagatgcc gaggaggagg tcctccggct tcagggagtt caggtagagg gagcggaacg
    24481 tgccgggatt gcacgggaag acggcgactc cggcgagcac gttgaagaag tacccggccg
    24541 tcagcgccgc gtggtaggag gtgccggagg cgacgaggaa gacgcggcca ccctccttct
    24601 gcgtcgtccg gatcgccgac acgagctcct ggaggagggt cgtgacgcgc cggcgcttct
    24661 tccagacgag gacgaggtcg aggagggcgg ccgcctccgc ggcgtcgggg acgaggcgga
    24721 cgccatcggc gaggagcgac ttctcgtccg agacgaacgg accgtaggag acgaggagct
    24781 cccggtgcgc gcggacccgg gcgacgagct ccgtcaggag cggctccccg aggaggtccc
    24841 ggaagccgcg ttcgaggccc gcctcgtccg tggccccgta gagcttcagc agcttctcga
    24901 cgagcgcctt gcagaggtcg acgcggtcct cgaacgcggc gaagagcccc tccgtctccg
    24961 gcgtccggaa gtaggtccgg acgatttcct cgaggttctc ggacgaggag gcgatctcct
    25021 ggtccatgaa gtaggcgaac ggcggccgga gccccgtgtc gcgcacgttc agcttcgagc
    25081 ggcgcggcct cggcgaggcg aagccgatcg gcccggcgag cggaaagacg gcgtacgccg
    25141 actcggtgaa ccagatccct tcgccctccg cgagcgggac gagcatccgg gtccgcgaga
    25201 ggaccgacgt caggtcgctc gagacgagga cgaagctccc cgtctcgtcc gcgccgatgc
    25261 cggcgtagag cgacgagccc gacttcaccg ccacgacccc ctccgaggcc gggtcggcgt
    25321 aggcggcggc gtaggagccc tcgacgccgg cgtcggcgcg gcgcgcggcc tcgatcagga
    25381 gaagcgcccg gtcggggacg gcgacgtcga gtcccgcggc cgcgagcgcc gcccgccccg
    25441 cggcgaggat ctgcgccgga tcggcgaggc cgccggcgaa cgcgtcctcc gcgaggtggg
    25501 tgagcgcctc tccgtcgttg tcggagacga cgtcgtgccc gcccgcggaa agccgcggcc
    25561 ggagcgtgtc ggtgctcgag atgttgccgt tgtgggcgcc gaccagctcg acgcggcacc
    25621 tcacgtggtg cggctgggcg ttcgcgtccg tgacggcccc gtaggtcgcc caccggacct
    25681 gtccgacgaa ccgctgcccc gcctcccggt cgatccccag ctcggggacg acccggctcg
    25741 gcgcgccgac cttcttgagg agcctcacgc tccggtccgc gcggacgaag gccgcgcccg
    25801 tcgagtcgta cccccggtac tccaggcgct tcagcagctc ccccgcctcg tgtccgaggc
    25861 cgggcacctc acgtccgtga cagaccgaaa cgatgccgca catcgtgctc tctcccctcg
    25921 ttccgactcc gtcgcacgac ccgtgcgcgg tcggggcgcc ccggccggcg gcatcgaggc
    25981 gtccccagcg acgcgtcgga ccgctcatct tgcctcagcc cgctcccacg cgcgagatcg
    26041 aaggccgccc cgacgaagcg ccccgagacc gcggttccga tcgcttgacc gcccgcctcc
    26101 atacgcctac aacggttgcg cgggaccact cccgcggagg agatcatgca gcctgtccgg
    26161 gacgccgagg cgctcgagcg agcgttcggg gagccgtggg ccctcgtctt caagcactcc
    26221 acccgttgcc ccgtcagctc ggcggcctgg cgggaggtgg cggcgttccg gaaggcgcat
    26281 ccggaggcgg tcgtttacgt cgtccacgtc ccggagcagc ggtcgctctc gaacgccctc
    26341 gccgcgcgga ccgggatccg ccacgagtcg ccgcaggcga tcctcctcga gaacgggcgc
    26401 gtcgcctggc acgactctca cgagggagtg accgccgagg cactcgggcg gctcctcccg
    26461 gcagccgtcg tccgctgacg ccgcgccgtt cgtctccgcg gcgttcgccc cggcgccgga
    26521 agcctcgtgc acctcccctt cgagaagtcc tcgccggatc aggtcccccc ccggaggcgt
    26581 atgattcttc cccggcgggg cgacggcgcc tcgcggcgcc cgatcgcctc gcgcgaggag
    26641 ttccgctctt gacgaggcgt ccccggctcc cccgcttcct cacaatccag acggcgcgcg
    26701 tcgcggccgt cctgatcgtc tgcctcgccg gacatcgcgc gacggggcgc gacctctcgc
    26761 tccccgaggc gcaggagcgt gtacgcgccg cccacgagg
//
LOCUS       DAOONL010000042        26622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1652_length_26622_cov_18.789739, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000042 DAOONL010000000
VERSION     DAOONL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_26622_cov_18.789739"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2435
                     /gene="ppdK"
                     /locus_tag="PLP50_08220"
     CDS             <1..2435
                     /gene="ppdK"
                     /locus_tag="PLP50_08220"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPA51573.1"
                     /translation="EALKELVGEFKKAIKEKTGHDFPEDPLAQLWGAVGAVFSSWMNE
                     RAIVYRQLNSIPESWGTAVNVQSMVFGNLGEDCGTGVAFTRDPATGENLFYGEYLINA
                     QGEDVVAGIRTPQKIATLQQAMPKVYDQLMGIRAKLEKHYRDMQDVEFTVERGRLFML
                     QTRTGKRTGFAEVRIAVEMEREKLISPEEAILRVNPDSLNQLLRPVFDPAAKKAAVSE
                     GQLLAKGLNAGPGAATGRVVFSAQDAFEWAKRGEKVLLVREETSPEDIKGMNAAEGIL
                     TARGGMTSHAALVARQMGKVCIVGCESLRIDYAQRCMTVRGDVKNRVVMEGDWLSIDG
                     STGEVLEGRIATKPSEVLQVVLEKSLAPEASPVYTLYATIMGWADKVRRLRVRANADQ
                     PDQATNAVEFGAEGIGLCRTEHMFFGGDRITAVREMILGDSVEAREKALAKLLPMQRS
                     DFEGVFRAMGPRPVTIRTIDPPLHEFLPHTLDEQAAVAEKLDVSSAAVAQKVSELHEM
                     NPMLGHRGCRLGIVFPEITRMQARAILEAACNVKKEGVEVKPEIMIPLVGHVNELKDQ
                     EKLVREVAKDVFAKAGIKVDYLVGTMIELPRAALTADEIATVAEFFSYGTNDLTQTTF
                     GLSRDDAGKFLPDYVKREILPSDPFEKIDQAGVGKLVKMGVELGRTTRPKLKIGICGE
                     HGGEPSSVEFCHRTSLDYVSCSPFRIPIARLAAGQAAVREGATVSGKPAKAAKAAAKP
                     APPKKAAPKAKPAAKPVKKAAKAKAPAKVAKKVAKKVAKAAPKAKPTPKRVAKPAPRK
                     PAKKAPAKKGRK"
     gene            complement(2602..3558)
                     /locus_tag="PLP50_08225"
     CDS             complement(2602..3558)
                     /locus_tag="PLP50_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51574.1"
                     /translation="MRIAKTTNGSPSLLWTLSPLLVVSLALSACSKSNVGSQPKASAD
                     ALTEQNRLLLAENEKLAERVRTSEAADAEATATLAEVQASLEEIRGKELKILRKTLDV
                     TREGQARTSTREMLADELATIRSAIRKNLDRIASLERERKADGRKMASLQTLVDELKR
                     SLEEKDVTIATLEKTVFELNDRVREQESVILVKDTLLREKDDVIATRTREVNTAFVAV
                     ADKKTLKEKGVVEKKGSILGLGGAWQETGKYDPQVFREIDTTVEAELTIPAPAEKVRV
                     LPGHPEESYQIVSGGPSSSTLKVTDREAFWRDSRYLVVMIPN"
     gene            3796..4392
                     /locus_tag="PLP50_08230"
     CDS             3796..4392
                     /locus_tag="PLP50_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51575.1"
                     /translation="MPVTPSVLAALRHALADPLSAAAVKAEVLATRLDREAPALSPRA
                     RDVGADLASAGRLLDLLAAVAAVAEEAPEESTLSSLVAGVGGEIGAGDAGLRLRVRPS
                     AAGLALRRLVDFGTARGSAPRVVAHASPDGAEVLVAPLGPASSAPEKLLLLPRGVAGA
                     DELFLARAAVEADGGSLRLTDRGGVLEARLLWPREEGR"
     gene            4389..5735
                     /locus_tag="PLP50_08235"
     CDS             4389..5735
                     /locus_tag="PLP50_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA51576.1"
                     /translation="MKALVVEDDPGARRALAELLVELGHEPAEVGTVAEALASLEGAP
                     PDVAIVDLGLPDGDGLDVVRAAPGATAVLVLTGQGSVRNAVEAMKAGAHDFLVKPLRL
                     PQLRAALELLAGRTSLPQARTVRVGEEGLGELLGGSPPMKEVFRLLARVAPSDAPVLL
                     WGESGTGKEVAARTLHSLSRRAAGPFVGVNCGAISPNLVESELFGHEKGAFTGAERRR
                     EGTFELARRGTLFLDEVTEMPLEMQVKLLRVLETKSFRRVGGAEERDVDVRVVASTNR
                     DVREAVASGVFREDLYYRLAVFPLRLPPLRERLGDVPRLALEFLARVEAEEGRGIEDI
                     EPEALALLGRHAWPGNVRELRNAIHRAYVLSDPPAISGEAAEAVLAAPAPSAPGGSSG
                     EPLAVPVKVGDTARVAERKLLEATLAAVGGDKRAAAEMLGLSLKTVYNKLRSWGVS"
     gene            complement(5713..7509)
                     /locus_tag="PLP50_08240"
     CDS             complement(5713..7509)
                     /locus_tag="PLP50_08240"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA51577.1"
                     /translation="MRTFARKERIVLGVAVAALAANAVLTVGHLDRLVEGQREMRRSD
                     QVLTELARVEATLGSAEAAQRRFLLARGATSLAPLEAGRTVLAIQLDRLAELTRGDDG
                     QAERVARLSLAARRQLALLNDGVRLYRQAGTEEPPREDPAESENAAAVRDALDGLEAS
                     ERRTLAAREAATARSRFAAGAAAFASTLLGFCLVLVGLLLLRRELVADAAATAALEEA
                     NAALRDADRRKDEFLASLAHELRNPLAAMRNAVELLDAPEEGGDGRARERRGLARSVL
                     RRQLAHLTHLVDDLLDLSRVTSSRLVLKKEAVALAEIVEAALETTRPALEARRHELSV
                     AFPRESVLVEGDPVRLAQVVSNLLSNAAKYSPPGGRIELAVAADEVEAVVRVTDDGIG
                     LAPRDLERVFGLFEQGSARPVDAEGLGVGLALSRRLAQLHGGSLTATSAGPGHGSTFT
                     LRIPRASAPEREAAPTAPPPAPSPACRVLLVEDNADGARSLAELLSIAGHEVHVAPDG
                     PSGLEAARTLLPDAVVLDVGLPGFDGYELARRLRAEPPLANVLLIALSGWAGRDDRAR
                     SLEAGIDHHLVKPAGVSRLAELLATVTTRPRT"
     gene            7675..8175
                     /locus_tag="PLP50_08245"
     CDS             7675..8175
                     /locus_tag="PLP50_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51578.1"
                     /translation="MRSSRAVVEERCRVASGALDTLLRAELAACEAYRTAIHSVERGG
                     ESPALALRSLYRMHRRFADEIQRLLSASDEEEPEGARAVWTDVARRVAGAPGGLDGAT
                     VLKRLQGGERSSLELALGTLRELSGRPAAFVRARYAPGLATNLELLSALLAHHGDEPA
                     ADEARP"
     gene            complement(8787..9707)
                     /locus_tag="PLP50_08250"
     CDS             complement(8787..9707)
                     /locus_tag="PLP50_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPA51579.1"
                     /translation="MTGAHTPGGAQRYALLSVGAAIATISLKLVAWHLTKSVGLLSDA
                     VESVVNLLAAVVALWALRVAAEPPDAEHHFGHEKAEYLASAFEGLAILGASIAIALTA
                     WQRIADPRPIESAWLGLTVSTVASAINGAVAFVLFRAGKRLDSITLRSDAHHLMTDVW
                     TSAGVLSGVALVQLTGWHILDPIVALAVATNIVWMAGKILVETAHGLLDKTIPEGEQA
                     SLETLLQKFRSGGVDLHAVRTRQAGPVRFVDMHVLVPGGWTVQRGHDLCEEIEREVRT
                     VLPRASVLTHLEPIEDPASWDDPGSPRRPR"
     gene            complement(9704..10585)
                     /locus_tag="PLP50_08255"
     CDS             complement(9704..10585)
                     /locus_tag="PLP50_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA51580.1"
                     /translation="MAHLVLALTVLVWSFSFLAAARLRQELGLGEALAARFVPVLLGA
                     GAYLLVRRPLRIPRAAWPTVVAMGLLSVPVYNVFFFHGLKSVPSGTAALVIALNPVFT
                     AVLARLVLGEPFGRRHVAGLALALAGVFVVIRYGTDKPVDWPYLSSALLLALAPLSWA
                     FYAVLGRRLPAEADPIDTSYALLFVGSLPLLAFARPSTVAKLAASPGALGSALYLAVP
                     CTLLAWVSFLWALKRLPAGEAAAFIFLNPPLANLWAFVLEGQTLRAPFLAGAAILLAG
                     VGLIVLRPATNRRRLRT"
     gene            10654..11541
                     /locus_tag="PLP50_08260"
     CDS             10654..11541
                     /locus_tag="PLP50_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA51581.1"
                     /translation="MALSAFFFALMAAGAKLLGGRVPPQEVILVRGVLNALFMLWLLR
                     RRGLSWRPKRPGLLVLRGALGHFALSCYFWSVARQPLATAVLLQQVHPVFTAVLAAWF
                     LSEKPGKRFLPAFLLAASGVALLVPRAAPGAAPASASLAPLVVGLLGAFLSAAAYVTV
                     RDASRTEHEETIVLWFPLTSIPLATVGTLVEGPAVPTPVEWGWLVFIAAAGQLGQLFL
                     TRGLARVPAGRATLANPLTVVFGALLGWLAFAEPIGTATLAGGAAIVAAVLLAGAGRA
                     SVSDARPPSPPAAGAPRGA"
     gene            11552..12193
                     /locus_tag="PLP50_08265"
     CDS             11552..12193
                     /locus_tag="PLP50_08265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPA51582.1"
                     /translation="MMRPRRRLGGLAAVLVLLVLAAVFAMSALRVGFSGTEAEMAAAR
                     AAAEAAGVPLDSWKTLVVASSGSVGAPAATAWKVFSRLEEWPTWSAPLHLGARWIGEP
                     GFRVAGGFEQVLALGFPLGTVRTREFVTRLTPGREVTWCRDEAGIVSCHLWRFTPVTP
                     TRTFVVNVEVLHGGPVGLFAPFVQSRWSRLFQGAVSGLARRAEGEAEKAATVP"
     gene            12190..13608
                     /locus_tag="PLP50_08270"
     CDS             12190..13608
                     /locus_tag="PLP50_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HPA51583.1"
                     /translation="MTTAALREGFEKADREKRFVALSSVLAAVFLTAMKVAVGVSTGS
                     IGILAEAAHSSLDLVAAVVTLWAVRAAGRPADPEHTYGHGKIENLSALFETALLLLTC
                     LWIVYESIERLFFHPAHVEPTAWAFAVMAISIVVDVSRSRALARTAKKYGSQALEADA
                     LHFSTDVWSSAVVLLGLALVVVSQRTGVAWLAKADAVAALVVAAIVVVLSLNLGRKSI
                     DDLVDAVPVGLKERLVAAARVPGVVDVPALRVRRTGPHAFVDATLTVGRHVAFERAHE
                     IADAAEAELKRLLPGADVVAHVEPVSEGNEGLLATTRLLAARHGLGAHAIRTHGTDGN
                     VSMELHLEVDEQLTVREAHDVVSVFEEDLRRAAPEVAHVVSHIEPVGETTAVRKTAPA
                     DDAPVRSELDRLRAERGPSFRPEGLAVRRGEDGLFVSFSLPVPGDLSVGEAHALSDEV
                     ERELKRRLGGGRVVIHVEPREE"
     gene            complement(13638..15512)
                     /locus_tag="PLP50_08275"
     CDS             complement(13638..15512)
                     /locus_tag="PLP50_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA51584.1"
                     /translation="MRPTTPPILLALVLALPAGGSELEVRAATLARVGGAWSPSFSPD
                     GTRLAFVSDLSGVPQAWTLPLTGGFPERVTPFEDPVVRVRWSPASALLAFAVAPGGAA
                     NEQVWVSRPDGTGLRLVTGGGKETNRLDGFTPDASRLRVASSGRSVAAIDAYLLDPLT
                     GEASARTSVPGESSFSDVSCDGRFALLARSSRAGDDDLFLVDLASGAELLLTNAGSPA
                     AVRQAALSPDASTVWLSTDAGRDLLAFARIRFAPNGAPGPVEFVAARDDAELSAFAVD
                     GAGLTAALVWSVAGRNELVFVDLATLRSRPGPALPAETASEPVFSPDGSRLALVLSGP
                     ATPSDVWVLERARGTFRQVTRSPHAGVDPGSLVKPTLVRLPGRDGLPISARLYRPRGA
                     TGPGPFVLSLEGGPETSEPGTFRADVHALVAEGISVLAPSVRGSAGFGRRFASLDDGP
                     LRRDAIEDVAACADWAVAAGLADPERIGLLGVSGGGHLAMAALAAFPRRFAAAATLHG
                     FADLPSFLRTTEPWLAAATKAEYGDPETDPLLLATLSPARRLDRVTTPTLVLHGANDT
                     ICPVEGAEALVRSLDRHGVPTRWVLFPDEGHGLRKTSNGVRATVEVVRWFTSYLGGRT
                     "
     gene            15578..16591
                     /gene="pdxA"
                     /locus_tag="PLP50_08280"
     CDS             15578..16591
                     /gene="pdxA"
                     /locus_tag="PLP50_08280"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPA51585.1"
                     /translation="MPEVRPVVAVTLGDPASIGPEVVVKALAGGELGAVCVPVVVGDR
                     RVVARALATTGAVLEVVPVERPGAATGKAGRVEVVDVPAYGIDAIDWGRVQAPAGRAA
                     YESIVVAIDLALAGEVDAVATAPVSKEALRAAGVSQIGHTEIFAERTGTLEPLTLFET
                     RGLRIFFLTRHLSLAEALRQVTAARVLATLRGAAAALRTLGVAAPRIAVAALNPHGGE
                     HGLFGDEEEKELVPAVLRAREEGIDASGPLPADSVFWHAAHGRFDAVLSLYHDQGHVA
                     AKMYDFERTVSMTCGLPFLRTSVDHGTAFDLAGTGRASATSMEEAIRAAARHAFAVRG
                     RTA"
     gene            complement(16592..17206)
                     /locus_tag="PLP50_08285"
     CDS             complement(16592..17206)
                     /locus_tag="PLP50_08285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51586.1"
                     /translation="MPRTPAPEKLYDAAYFARWYHDPRERAARAAVVRRKAALALAAA
                     EHLLERPLRNVLDVGCGEAPWRAALLRLRPRLRYVGVEPSSWASARGAARGVVRGGLD
                     DLERLSLGGPFDLVVCSDVLHYVGDAQLRRGLAAMASLARGLLWLEAFTSEDDFEGDV
                     DGFVRRSPRAYRSVFRRAGLVPLGLFLHASPALAPRVVALERGS"
     gene            17350..18147
                     /locus_tag="PLP50_08290"
     CDS             17350..18147
                     /locus_tag="PLP50_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPA51587.1"
                     /translation="MKTILRISLGALLLLPLAATAQWEGSLEMKISGKDANGTSKALV
                     GKAGARMQIEMTNAQLRQAGQGAMKMTILTRTSEPDVSYMLNDAQKSYARIDVKEMQE
                     AIPASEREKKWAVKRLGSDRVAGYACERALVSEEGGETENEVCVSKEILGSAGLLSAG
                     RRDASAHGLEKALKANGLEGMPIRMAIRQRGKSEATMTWELVKAEKKALPASTFEIPA
                     GYRETSMMGTMMSPEQQKQIDDAMKNLTPEQRKMFEQMMKKQGGGGR"
     gene            complement(18220..19590)
                     /gene="mgtE"
                     /locus_tag="PLP50_08295"
     CDS             complement(18220..19590)
                     /gene="mgtE"
                     /locus_tag="PLP50_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPA51588.1"
                     /translation="MRIGELLGPDVLEIVREDPAALREGLLEFHPADVADLLAAIPRE
                     DRIPVLEQLHEEQLGGVLSYLGGGVLRMGLTRLPPAKIAQALDSLEPDDAARLLSFLP
                     EERRLPVLARMSAKDAAAARGLLAWEPGTAGRLMTDRFVKVSPDWSVSETMAHLRKID
                     PEVATVADLYAVDEKGRLVGVTSLRKLFPAEPGRKVWELMTHDVISVAPDTPQEEVAR
                     LVAKYGFNAMPVVGEDGKALGVVTVDDVVDVLVARETEAALAMGAVAAPSDPDEKHEF
                     EYFGAPLWRVVKSRLGWLLLLFIAGTLTGSVLKHFEGELSKVVALSFFIPLIIGTGGN
                     AGSQTVSTIIRALALKQVKVKDALRVCLREMSSGLILGGILCAVAVGRVSLWGVGGSL
                     ALTVGLTILAVCVWANAIAALVPLLAERFDFDPTVASAPLITTLVDATGLAIYMVIAK
                     AILPGI"
     gene            19722..21149
                     /locus_tag="PLP50_08300"
     CDS             19722..21149
                     /locus_tag="PLP50_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51589.1"
                     /translation="MSAEDGRVLVVDDDPVNRAILVGSLEAQGYAAGMAADGREALRL
                     LRTDGFDVVLLDLVMPVMDGFEVLRTIKADERLRSVPVIVVSALEEMESVVRCIEMGA
                     ADYLAKPFDPVLLRARLRASLASKRLDDVVAAHLLEIEELAEQLKTRNRFIQETFGRY
                     LSEAVVAELLESPDGLRLGGEKRKVTMLMSDLRGFSAIAERLEPEQVVRVINNYLGTM
                     ADVILSWDGTIDEFIGDSVLGFFGAPVARPDDARRALACALAMQKGMEDVNARNRSEG
                     LPAVEMGIAVHTGEVVVGNIGSEKRAKYGAVGSQVNLLARIEAFTCGGQVLISERTLG
                     EAGHGIVTGLSLAVRAKGFPEPLRVHDLLGLVEAPHLHLGRRAEEMRPVDPPLKAVFT
                     VLEGKHVARVEEPAEVVALSPRGAAILTSATVEVLSDLKLRIVGPGGLLPGELYAKVL
                     PSPDGGPVRLRFTSVPVELEPLLSP"
     gene            21180..21728
                     /locus_tag="PLP50_08305"
     CDS             21180..21728
                     /locus_tag="PLP50_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPA51590.1"
                     /translation="MPRPTRRDRAGTFGLVVGLGDLTLEPVDAIVNAANPSLLGGGGV
                     DGAIHAAAGPGLLAVCAGIGGCPPGEARMTGGHRLPARHVIHTVGPVWRGGGAGEDET
                     LACCYRASLRLAEEAGLASVAFPSIATGIYGFPIERASRIALREIRAFLAASRCVRSV
                     KVVTFSERDLAAYRAAEKELAS"
     gene            21725..22417
                     /locus_tag="PLP50_08310"
     CDS             21725..22417
                     /locus_tag="PLP50_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPA51591.1"
                     /translation="MTDRAGRAAVLVAGATGLVGGALLRRLLATPGGPRVVAVVRRPL
                     EPVSPRPDVVVADFARLERVDLPRAGTAFCALGTTMAKAGSREAFRAVDLDAVVAFAR
                     AARRAGATRFLGVSALGADPRSRVFYNRVKGEAEEALRALGFEGLALVRPSLLLGERP
                     ETRPAERAAAAVTRIVPWNLAGPLAKWRPVPADAVAAALLAIAGQPLRGVRVVENEEI
                     HRIASSDPLSVR"
     gene            22428..23189
                     /locus_tag="PLP50_08315"
     CDS             22428..23189
                     /locus_tag="PLP50_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPA51592.1"
                     /translation="MSSDTPGFPPAPPLPPPPMPPPPAGGGWQTPPPGGPALAGLPWE
                     HRAELGFLNAFVETIKLFVTNPADAWRRTKEKGDFAEPLIFAILVSWIGIAVSTLWSG
                     LFGQAWISFLPPELKEKMAGAFATNAAALMIQVGLAPIFVLIGLFVGAAFYHLSFMIV
                     GATKDSTSGFEGTFRVVSYASVSQLANVIPFVGGLAAVVWNLVLVVLGVVRMHRTTQG
                     KAIAGVLIPLLLCCVCIVVVVALVIGGLAAAFASR"
     gene            23194..23622
                     /locus_tag="PLP50_08320"
     CDS             23194..23622
                     /locus_tag="PLP50_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPA51593.1"
                     /translation="MTDVARGAAGAGRPPAVQLGLLWGAAAVSAVALAVSFRGSVATS
                     LALLPPCPFKSVTGLPCPACGSGRASAALATLDLPSALASNPLFTFGAFVFLLGGLFA
                     AAAAFTGRGVPEPRTLPVPVRAAVVGVVAANWLWLLLDGR"
     gene            23630..24088
                     /locus_tag="PLP50_08325"
     CDS             23630..24088
                     /locus_tag="PLP50_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51594.1"
                     /translation="MPRASADPATDFRMLCDALFEFVARHPQAGPKMADLGISGRGVF
                     PDLKLVLNVAPAGAKLRARGVHVTWSWGAAPKGFAPKVLVSCPTAVANRLFQGKENPA
                     LALDRGDLTLSALDPSVNERRLLNLLPVLVPLRTEVPGVLRKAGLARLIL"
     gene            complement(24002..24973)
                     /locus_tag="PLP50_08330"
     CDS             complement(24002..24973)
                     /locus_tag="PLP50_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51595.1"
                     /translation="MEALTARIAARFLVGTAALLGAAVAVSAPPRLVVEAPPSLAAYA
                     REVERLDPVAFLPAMELTGLFEPGDVRVVVTAEGSALATRAPSWAVAYAAPAEGTVVL
                     IPTRTPSWPDDGLPDVVRHEVAHVLTFRAAGGRQVPRWFDEGLAMAAGRRRVIEDDLR
                     EALAVLTGPRLRFAEVDALFLGGRSDANRAYALSSAFFGDLLARHGSAFPKRVLRRMA
                     GGELFEEAFRSATGVPFRDESGAFWGSRRSLERWLPIVTSTAAVWTAIVLLTLWAVRV
                     ARGRRHARLAEWEAAERAALQDQPREAGLPEDARDLGPEGDEDREEI"
     gene            complement(24961..25836)
                     /locus_tag="PLP50_08335"
     CDS             complement(24961..25836)
                     /locus_tag="PLP50_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPA51596.1"
                     /translation="MKILIADAFDDSLPGRLAPFGEVTQDAAKTAEADVLLVRSKTKV
                     TKEYLEGAPNLKLIIRGGVGLDNIDVQAAREKGIEVKNTPRASSIAVAELAMALMLTV
                     PNRVLEGHNGIKEGKFLKKELKRTELYGKTLGLLGAGLIGTEVARRAIAFGMRVVAHD
                     HYISNHALADMVEDDDELFALSDYISIHLPLTPETKGMLDKNAFAKMKDGVILINTAR
                     GKVVVEEDLAEALKSGKVRAYATDVFYSDPPDPSCPLLTAPNVVMTPHIGGSSKENLL
                     RIGDNVVDILKKWKR"
     gene            complement(26117..>26622)
                     /locus_tag="PLP50_08340"
     CDS             complement(26117..>26622)
                     /locus_tag="PLP50_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HPA51597.1"
                     /translation="VAAAAMTKNPVRQALVSSTGTFWDTVVVCAMTGLVVVNSGDWHE
                     GLAGAALTKTAFSHIPHVGPAVLTVGLLTFVFSTILGWSYYGEKAAEYLFGPKVVRPY
                     RLLWVVAVLVGSVATLPVVWSFADIANGLMAIPNLISLLALSGVLAAETRKYLAPGMI
                     DTAATEE"
BASE COUNT         3608 a         9343 c        10081 g         3590 t
ORIGIN      
        1 tcgaggcgct gaaggagctc gtcggcgagt tcaagaaggc gatcaaggag aagaccggtc
       61 acgacttccc cgaggacccg ctggcccagc tctggggcgc cgtcggggcg gtcttctcgt
      121 cctggatgaa cgagcgggcg atcgtctacc gccagctcaa ctcgatcccc gagagctggg
      181 ggacggccgt gaacgtccag tcgatggtct tcggcaacct cggcgaggac tgcggcacgg
      241 gcgtcgcctt cacgcgcgac cccgccacgg gcgagaacct cttctacggc gagtacctca
      301 tcaacgccca gggcgaggac gtcgtcgccg gcatccggac gccccagaag atcgcgaccc
      361 tccagcaggc gatgccgaag gtctacgacc agctcatggg catccgcgcg aagctcgaga
      421 agcactaccg cgacatgcag gacgtggagt tcacggtcga gcgcggccgg ctcttcatgc
      481 tccagacgcg caccggcaag cggaccggct tcgccgaggt gcggatcgcc gtcgagatgg
      541 agcgcgagaa gctcatctcg cccgaggagg cgatcctccg cgtcaacccc gactcgctga
      601 accagctcct ccgcccggtc ttcgaccctg ccgccaagaa ggccgccgtc tccgagggac
      661 agctcctcgc gaagggcctc aacgccggcc cgggcgccgc caccggccgc gtcgtcttct
      721 ccgcccagga cgccttcgag tgggcgaaga ggggcgagaa ggtcctcctc gtccgcgagg
      781 agacgagccc cgaggacatc aaggggatga acgcggccga ggggatcctc acggcccgcg
      841 gcggcatgac ctcccacgcg gcgctcgtcg cgcggcagat ggggaaggtc tgcatcgtcg
      901 gctgcgagtc gctccggatc gactacgccc agcgctgcat gaccgtccgc ggcgacgtga
      961 agaaccgcgt cgtgatggag ggcgactggc tctccatcga cggctcgacg ggcgaggtcc
     1021 tcgaggggcg gatcgcgacg aagccgtccg aggtcctcca ggtcgttctc gagaagagcc
     1081 tcgcccccga ggcgagcccg gtctacacgc tctacgcgac gatcatgggc tgggccgaca
     1141 aggtccggcg cctccgcgtc cgcgcgaacg ccgaccagcc cgaccaggcc acgaacgcgg
     1201 tcgagttcgg cgccgagggg atcggcctct gccgcaccga gcacatgttc ttcggcggcg
     1261 accggatcac ggccgtccgc gagatgatcc tcggcgacag cgtcgaggcg cgcgagaagg
     1321 cgctcgcgaa gctgctcccg atgcagcgca gcgacttcga gggggtcttc cgcgcgatgg
     1381 gcccgcggcc cgtcacgatc cggacgatcg acccgccgct ccacgagttc ctcccgcaca
     1441 ccctcgacga gcaggcggcc gtcgccgaga agctcgacgt ctcgtccgcc gccgtggccc
     1501 agaaggtgag cgagctccac gagatgaacc cgatgctcgg ccaccgcggc tgccgcctcg
     1561 ggatcgtctt ccccgagatc acgcggatgc aggcgcgcgc catcctcgag gcggcctgca
     1621 acgtgaagaa ggagggggtc gaggtcaagc cggagatcat gatcccgctc gtcggccacg
     1681 tgaacgagct gaaggaccag gagaagctcg tccgcgaggt cgcgaaggac gtcttcgcga
     1741 aggccggaat caaggtggac tacctcgtcg ggacgatgat cgagctgccg cgcgcggcgc
     1801 tgaccgccga cgagatcgcc acggtcgccg agttcttcag ctacggcacg aacgacctga
     1861 cgcagacgac gttcggcctc agccgcgacg acgccgggaa gttcctgccc gactacgtca
     1921 agcgcgagat cctcccctcc gacccgttcg agaagatcga ccaggccggc gtcggcaagc
     1981 tcgtgaagat gggcgtcgag ctcggccgga cgacccgccc gaagctgaag atcggcatct
     2041 gcggcgagca cggcggcgag ccgtcgtccg tcgagttctg ccaccggacg agcctcgact
     2101 acgtcagctg ctcgccgttc cggatcccga tcgcccgcct cgccgccggc caggccgccg
     2161 tccgcgaggg cgcgacggtc tccgggaagc cggcgaaggc cgcgaaggca gccgcgaagc
     2221 cggctccccc gaagaaggcg gcgccgaagg cgaagcccgc ggcgaagccg gtgaagaagg
     2281 ccgcgaaggc gaaggcgccg gcgaaggtcg cgaagaaggt cgcgaagaag gtcgccaagg
     2341 cggccccgaa ggcgaagccg accccgaaga gggtcgcgaa gcccgccccg aggaagcccg
     2401 cgaagaaggc cccggcgaag aagggccgga agtagcacca cgccccgcgc cggcaggtcc
     2461 ggcgcggggc gtccgagccc gggagacgac gacggcggcc gaacggccgc cgtcgctctc
     2521 tccggggacg tcccttcgcg aagaggcccg cggcggccga tcggccgccg cgggcggggc
     2581 gcaggagccg ggagagccgg gtcagttcgg gatcatgacg acgaggtacc ggctgtcgcg
     2641 ccagaaggcc tcacggtccg tgaccttcag cgtcgacgag ctcggtcctc cggagacgat
     2701 ctgatagctc tcctccggat gcccgggaag gactcggacc ttctccgcgg gcgccgggat
     2761 cgtgagctcc gcctccacgg tggtgtcgat ctcgcggaag acctgcgggt cgtacttccc
     2821 ggtctcctgc caggcgccgc cgaggccgag gatcgagccc ttcttctcga cgacgccctt
     2881 ctccttcagc gtcttcttgt cggcgaccgc gacgaacgcg gtgttgactt cgcgggtcct
     2941 ggtcgcgatg acgtcgtcct tctcgcgcag gagcgtgtcc ttcacgagga tcacgctctc
     3001 ctgctccctc accctgtcgt tcagctcgaa gacggtcttc tcgagggtcg cgatcgtgac
     3061 gtccttctct tccagcgatc gcttcagctc gtcgacgagc gtctggagag aggccatctt
     3121 ccggccgtcc gccttccgct cacgctcgag cgacgcaatc cggtcgaggt tcttgcggat
     3181 cgcgctccgg atcgtcgcga gctcgtcggc gagcatctcc cgggtgctcg tccgagcctg
     3241 tccctcgcgc gtcacgtcga gggtcttcct gaggatcttc agctccttgc cgcggatctc
     3301 ctcgaggctc gcctggactt cggcgagagt cgcggtcgcc tcggcgtccg cggcctccga
     3361 ggtccggacc cgttccgcga gcttctcgtt ttcggcgagg aggagccggt tctgctcggt
     3421 gagcgcatcc gcgctcgctt tcggctggct tccgacgttc gacttcgagc aggccgacag
     3481 ggcgagcgag acgacgagga ggggggagag ggtccagagc agcgacggtg agccgttggt
     3541 ggtcttcgcg attctcatgg tggacctccg tgaccggcgt cctttcaagg gccgcgccag
     3601 cggctgccgc tccgaccgga gccgcgcaag ccgtgaatga cgagggcgtt gcccgcgccg
     3661 cgcgcctccg atcgggatcg ggccgccgga ggaaggggag gaagcgatta caaagggcgt
     3721 gtaagaagcg gactcgccgg ccggacgatc cccgcggggg tgaccgggca cccggctcgc
     3781 tggtagcgtt ggcgcatgcc cgttaccccg tcggtcctcg cggccctgcg tcacgccctc
     3841 gccgacccgc tctcggcggc ggcggtgaag gccgaggtcc tcgcgacgcg gctcgaccga
     3901 gaggcgcccg ccctctcacc gagggcccgc gacgtcggcg ccgacctcgc gtccgcaggc
     3961 cggctcctcg atctcctcgc cgccgtcgcg gcggtcgccg aggaggcgcc ggaggaatcg
     4021 acgctctcct cgctcgtcgc gggagtcggc ggcgagatcg gcgcgggaga cgccggcctc
     4081 cgtctgcgag tccgcccgtc cgccgccggc ctggctctcc ggcggctcgt cgacttcggc
     4141 accgcgcgag ggtccgctcc gcgcgtcgtg gcgcacgcct cgccggatgg ggcggaggtc
     4201 ctcgtcgcgc cgctcggccc ggcctcgagc gccccggaga agctgctgct cctgccgcgg
     4261 ggcgtcgcgg gcgccgacga gctcttcctc gcgcgggccg ccgtggaggc ggacggggga
     4321 tcgctccggc tcacggatcg tggaggcgtg ctcgaggcga ggctcctctg gccgcgcgag
     4381 gagggacgat gaaggcgctc gtcgtcgagg acgaccccgg ggcgcgccgg gcgcttgccg
     4441 agctgctcgt cgagctgggt cacgagccgg cggaggtcgg aaccgtggcg gaagcgctcg
     4501 cgtctctcga gggggccccc cccgacgtcg cgatcgtcga cctcgggctg cccgacggag
     4561 acgggctcga cgtcgtgcgg gccgcccccg gcgcgacggc cgtcctcgtc ctgacgggcc
     4621 aggggtccgt ccggaacgcc gtcgaggcga tgaaggcggg cgcgcacgac ttcctcgtga
     4681 agcctctgcg cctgccgcag ctgcgcgcgg cgctggagct cctcgccggg cgcacctcgc
     4741 tcccgcaggc ccggacggtg cgggtcgggg aggagggtct cggcgagctc ctcggaggat
     4801 cgcccccgat gaaggaggtc ttccggctcc tcgcgcgggt cgccccgtcc gacgccccgg
     4861 tcctcctctg gggcgagagc gggacgggga aggaggtcgc cgcgcggacg cttcactccc
     4921 tctcaaggcg cgcggccggc cccttcgttg gcgtgaactg cggcgccatc tcgccgaacc
     4981 tcgtcgagag cgagctcttc gggcacgaga agggggcctt cacgggagcg gagcggcggc
     5041 gggaggggac gttcgagctc gcccgccgcg gcacgctctt cctcgacgaa gtgaccgaga
     5101 tgccgctgga gatgcaggtg aagctcctgc gggtcctcga gacgaagtcg ttccggcgcg
     5161 tcgggggcgc ggaggagcgc gacgtggacg tccgcgtcgt ggcgagcacg aaccgcgacg
     5221 tccgggaagc cgtggcgagc ggcgtcttcc gggaggacct ctactaccgg ctcgccgtct
     5281 tcccgcttcg cctccctccg ctccgcgagc ggctcggcga cgtcccgcgc ctggccctcg
     5341 agttcctcgc gcgcgtcgag gccgaggagg ggcgcgggat cgaggacatc gagccggagg
     5401 cgctcgcgct gctcgggcgc cacgcctggc ccgggaacgt ccgcgagctg aggaacgcga
     5461 tccaccgcgc ctacgtcctc tccgatcctc ccgcgatctc cggcgaggcc gcggaggcgg
     5521 tcctcgccgc gccggcgccg tcggcgccgg gagggtcgtc gggagagccg ctcgccgttc
     5581 ccgtgaaggt cggcgacacg gcgcgggtcg ccgagcggaa gctcctggag gcgacgctcg
     5641 cggccgtcgg aggagacaag agagcggccg ccgagatgct cgggctgtcg ctgaagaccg
     5701 tctacaacaa gctcaggtcc tggggcgtgt cgtgaccgtc gcgagaagct cggcgaggcg
     5761 ggagacgccc gcgggcttga cgaggtggtg gtcgatcccc gcctccagcg agcgcgcccg
     5821 gtcgtcgcgc ccggcccagc ccgagagcgc gatcaggagg acgttcgcga gcggaggctc
     5881 cgccctcagg cggcgcgcca gctcgtagcc gtcgaagccc gggaggccga cgtcgaggac
     5941 gacggcgtcc gggagcagcg tgcgggcggc ctccagcccg gacggtccgt ccggcgcgac
     6001 gtggacctcg tggccggcga tcgagaggag ctcggcgagc gaccgcgccc cgtcggcgtt
     6061 gtcctcgacg aggaggaccc gacaggccgg cgacggggcg ggaggcgggg ccgtgggcgc
     6121 ggcctcgcgc tcgggcgcgg acgcccgcgg gatccggagc gtgaacgtgc tgccgtggcc
     6181 cgggccggcg ctcgtcgcgg tcaggctccc gccgtggagc tgggcgaggc gccgcgagag
     6241 cgcgaggccg accccgagcc cctcggcgtc gaccgggcgc gccgagccct gctcgaagag
     6301 gccgaagacc cgctcgaggt cccggggcgc caggccgatg ccgtcgtcgg tgacccgcac
     6361 gacggcctcg acctcgtcgg cggcgacggc gagctcgatc cggccgcccg ggggcgagta
     6421 cttcgcggcg ttcgagagga ggttcgagac gacctgcgcg agacggaccg ggtcgccttc
     6481 gacgaggacg ctctcgcgcg ggaacgcgac ggagagctcg tgccgccgcg cctcgagagc
     6541 ggggcgcgtc gtctcgagcg cggcctcgac gatctcggcc agggcgacgg cctccttctt
     6601 caggacgagc cggctgctcg tgacgcgcga gaggtcgagg aggtcgtcga cgaggtgcgt
     6661 gaggtgagcg agctggcgcc ggaggacgga gcgcgcgagt ccccgccgtt cccgcgccct
     6721 gccgtcgccg ccctcttccg gagcgtcgag cagctccacg gcgttccgca tggccgcgag
     6781 cgggttgcgc agctcgtgcg cgagggaggc gaggaactcg tccttgcggc ggtcggcgtc
     6841 gcggagcgcg gcgttcgcct cctcgagggc cgcggtcgcg gcggcgtcgg cgacgagctc
     6901 gcggcgcagg aggaggagcc cgacgaggac gaggcagaaa ccgaggagcg ttgaggcgaa
     6961 cgccgcggcg ccggcggcga atcggctcct cgccgtcgcg gcctcccgcg ccgcgagcgt
     7021 ccgccgctcg gaggcctcga gcccgtcgag ggcgtcgcgg acggcggcgg cgttctcgct
     7081 ctccgccggg tcctcgcgcg ggggctcctc cgtcccggcc tgccggtaga ggcgcacgcc
     7141 gtcgttgagg agggcgagct gccgacgggc cgcgagggag agccgggcga cgcgctcggc
     7201 ctgaccgtcg tcgccccgcg tcagctccgc gagccggtcg agctggatgg cgaggaccgt
     7261 tctccccgcc tccagcgggg cgagagaggt cgctcccctc gccaagagga accggcgctg
     7321 ggcggcctcg gcggagccga gcgtcgcctc gaccctcgcc agctccgtca ggacctggtc
     7381 ggatcgccgc atctcccgct gtccctcgac gaggcggtcg aggtgtccga ccgtcaggac
     7441 ggcgttcgcg gcgagcgccg cgacggcgac gccgaggacg atgcgctcct tgcgggcgaa
     7501 agtccgcatg gaaagatctt ctctcggacg gcaccgtttt caaggtcgcg ggcgtgggag
     7561 cggcgcgagg acgggctgcg ccggccgcgg cgccgtcgcg cggtggcgcc cgccttgcac
     7621 ctcccccgga gccgggaagc ggccctgccg cgagcccggg acgggaggtg cgacatgaga
     7681 tcgagccgtg ccgtcgtcga agagcgctgt cgcgtcgcgt ccggagcgct ggacactctc
     7741 ctccgggccg agctcgcggc ctgcgaggcg taccggaccg cgatccactc ggtcgagcga
     7801 ggtggcgaga gccccgcact cgcccttcgc agcctctatc ggatgcaccg gcgcttcgcc
     7861 gacgagatcc agcggcttct ctcggcctcg gacgaggagg agcccgaggg tgcccgtgcc
     7921 gtctggacgg acgtggcgcg tcgcgtcgcg ggcgccccgg gcgggctcga cggcgcgacg
     7981 gtcctgaagc gcctgcaagg cggagaacgt tcgtcgctgg agctcgcgct cggaacgctc
     8041 cgggagctct ccggccggcc ggccgccttc gtccgggctc ggtacgcgcc gggcctggcg
     8101 acgaacctcg agctcctctc ggcgctcctg gcgcaccacg gagacgagcc ggcggccgac
     8161 gaggcgcgac cgtgatggca gagcgcttgc gtggagatcg gtcagaggat gccgaaaggc
     8221 gcgaccctcg aggaggtgaa cggagcggcc gggccgcgaa cgagacgaaa cgacagtggg
     8281 aaggatcccg gtaccggtat cccccccggg acgaggccca taaagccagc aggcgagatc
     8341 ggatgaggcg agcgcgagcg agccggaccg ccgcctgcgg gcccccgagg tccttcccgc
     8401 ttctccccca ggaggcggcg cttcccccgg cgccgccccc tttttttggg ctcgcctgaa
     8461 cctctggcgg cgtccgggat cggcccggga gagaccctcg cgccgcggcc gggacggaag
     8521 cggctttcct cgctccggcc ggcccctcgg cccgggaccg accgggcgac cttcgatgac
     8581 gcggcgcgag cggcgagaac cttcacggag acggatcgct cgccgtctgg caggtgtatc
     8641 tcgacgacgc gggcgcgggc gccgacgccg acctccgggg cgacctttgc agcacctcct
     8701 gcggctgcgg gaggccggcg ccaggggcac cgatccgcgg gagccgcacc ggcggcagga
     8761 gccgcaaagg gcatgccccg gagcggtcag cgcgggcggc ggggggagcc cgggtcgtcc
     8821 caggaggcgg ggtcctcgat cggctcgagg tgggtgagga cggaggcgcg ggggaggacc
     8881 gtgcgcacct cgcgctcgat ctcctcgcag aggtcgtgac cgcgctgcac cgtccagccg
     8941 cccggaacga ggacgtgcat gtcgacgaag cggaccggtc cggcctgcct cgtcctcacg
     9001 gcgtggaggt cgacgccccc gctccggaac ttctggagga gcgtctcgag cgaagcctgc
     9061 tcgccttcgg ggatcgtctt gtcgaggagg ccgtgcgccg tctcgacgag gatcttcccg
     9121 gccatccaga cgatgttcgt cgccacggcg agggcaacga tcgggtcgag gatgtgccag
     9181 ccggtgagct ggacgagggc gacgcccgag aggacgccgg ccgaggtcca gacgtcggtc
     9241 atcaggtggt gcgcgtcgga gcggagcgtg atcgagtcga ggcgcttccc cgcccggaag
     9301 aggacgaacg cgaccgctcc gttgatcgcg gaggcgacgg tcgagacggt gaggccgagc
     9361 caggccgact cgatcggcct cgggtcggcg atccgttgcc aggcggtgag cgcgatcgcg
     9421 attgaggcgc cgaggatcgc gaggccttcg aaggcggagg cgaggtactc ggccttctcg
     9481 tgcccgaagt ggtgctcggc gtcgggcggc tcggccgcga cccggagggc ccacagggcg
     9541 acgacggccg cgaggaggtt gacgaccgac tcgacggcgt cggagaggag gccgacggac
     9601 ttcgtcaggt gccaggcgac gagcttcagc gagatcgtcg cgatcgcggc gccgacggag
     9661 aggagcgcgt agcgttgggc gccgccgggg gtgtgcgcgc cggtcacgtt cggagcctac
     9721 gccggttcgt cgcggggcgg aggacgatca gcccgacgcc ggcgaggagg atcgcggctc
     9781 ccgcgaggaa cggagcccgc agcgtctgcc cttcgaggac gaacgcccag aggttcgcga
     9841 gcggcgggtt gaggaagatg aaggccgccg cctcgccggc ggggagcctc ttcagcgccc
     9901 agaggaacga gacccaggcg aggagcgtgc aggggacggc gaggtagagc gccgacccga
     9961 gcgccccggg gctcgcggcg agcttcgcga ccgtcgacgg tcgcgcgaac gccaggagag
    10021 ggaggctccc gacgaagagg agcgcgtacg acgtgtcgat cgggtccgcc tcggcgggga
    10081 gcctccgtcc gaggaccgcg tagaacgccc aggagagcgg ggcgagggcg aggaggagcg
    10141 cgctcgagag gtaaggccag tcgacgggct tgtccgtgcc gtagcggatg acgacgaaga
    10201 cgccggcgag cgcgagggcg agtccggcga cgtgccgccg gccgaacggc tcgccgagga
    10261 cgaggcgggc gaggacggcg gtgaagacgg gattgagcgc gatgacgagc gccgccgtgc
    10321 cgctcgggac cgacttcagc ccgtggaaga agaagacgtt gtagacgggg accgagagga
    10381 ggcccatcgc gacgaccgtc ggccaggccg cgcgcgggat ccggagcggg cggcgcacga
    10441 ggaggtatgc gccggccccg aggagcaccg ggacgaaccg cgccgcgagc gcctccccga
    10501 ggccgagctc ctggcgcagg cgcgccgccg ccaggaacga gaacgaccag acgaggaccg
    10561 tcagggcgag gacgaggtgg gccaagggag gcggagtatc gcaccggcgc gccgcggggc
    10621 gcccctcgcg gataatcgcc ggccgtgcgc gagatggccc tctccgcctt cttcttcgcc
    10681 ctcatggcgg cgggggcgaa gctcctcggc gggagggtac cgccgcagga ggtgatcctc
    10741 gtccggggcg tcctgaacgc gctcttcatg ctctggctgc tccgcaggcg cggcctctcg
    10801 tggcggccga agcggcccgg cctcctcgtc ctgcgtggcg ccctcgggca cttcgcgctc
    10861 tcctgctatt tctggtccgt cgcccggcag ccgctcgcca ccgcggtcct cctccagcag
    10921 gtgcaccccg tcttcacggc ggtcctcgcc gcctggttcc tctcggagaa gccggggaag
    10981 cgcttccttc cggcgttcct cctcgcggcg tccggcgtcg cgctcctcgt cccgcgggcc
    11041 gcgccggggg cggcccctgc gtccgcctcg ctcgcgccgc tcgtcgtcgg gctcctcggg
    11101 gcgttcctgt cggcggcggc ttacgtcacc gtccgcgacg cctcccgcac ggagcacgag
    11161 gagaccatcg tcctctggtt cccgctcacg tcgatcccgc tcgcgacggt cgggacgctc
    11221 gtcgaggggc ccgcggtgcc gacgccggtc gagtggggat ggctcgtctt catcgccgcg
    11281 gccggccagc tcggccagct cttcctcacg cggggcctcg cgcgggtgcc cgccgggagg
    11341 gcgacgctcg cgaacccgct caccgtcgtc ttcggcgcgc tcctcggctg gctcgcgttc
    11401 gccgagccga tcgggaccgc gacgctcgcc ggcggcgcgg cgatcgtcgc cgcggtcctc
    11461 ctggccggag ccgggcgggc gtcggtctcc gacgcgcggc cgccgtctcc tcccgcggcc
    11521 ggcgccccgc gaggggccta aactcgtccg gatgatgcgt ccccgtcgcc gtctcggcgg
    11581 tcttgccgcg gtcctcgtcc tcctcgtcct ggcggcggtc ttcgcgatgt ccgcgctgcg
    11641 cgtcggcttc tcggggacgg aggccgagat ggccgctgcg cgtgccgccg cggaagcggc
    11701 gggcgttccg ctcgattcct ggaagacgct cgtcgtcgcc tcctcgggga gcgtcggagc
    11761 gcccgcggcg acggcgtgga aggtcttctc ccgtctcgag gagtggccga cgtggtcggc
    11821 accgctccac ctcggggcgc gctggatcgg cgagccgggc ttccgggtcg ccggcggctt
    11881 cgagcaggtc ctggctcttg gcttcccgct cgggaccgtg cggacccggg agttcgtcac
    11941 gcgcctcacg cccggccgcg aggtgacgtg gtgccgggac gaggccggga tcgtctcctg
    12001 ccacctctgg cgcttcactc cggtcacgcc gacccggacg ttcgtcgtga acgtggaggt
    12061 cctccacggc ggcccggtcg gcctcttcgc gccgttcgtc cagtcccgct ggagccgcct
    12121 cttccagggg gccgtctcgg gcctcgcacg ccgggccgag ggtgaggcgg agaaggccgc
    12181 gaccgtcccg tgacgaccgc ggcgcttcgg gaaggcttcg agaaggccga ccgggagaag
    12241 cgcttcgtcg cgctctcctc cgtcctggcg gcggtcttcc tgacggcaat gaaggtcgcc
    12301 gtcggcgtct cgaccggctc gatcggcatc ctcgccgagg cggcccactc gtcgctcgac
    12361 ctcgtcgccg cggtcgtgac cctgtgggcc gtgcgggccg cggggcgacc cgccgacccg
    12421 gagcacacct acgggcacgg gaagatcgag aacctctcgg cgctcttcga gaccgcgctg
    12481 ctcctcctga cctgcctctg gatcgtctac gagtcgatcg agcgtctctt cttccacccg
    12541 gcgcacgtcg agccgaccgc ctgggccttc gccgtgatgg cgatctcgat cgtcgtcgac
    12601 gtctcccgtt cgcgggcgct cgcgcgcacc gcgaagaagt acgggagcca ggcgctcgag
    12661 gcggacgcgc tccacttctc gacggacgtc tggtcctcgg ccgtcgtcct cctggggctc
    12721 gcgctcgtcg tcgtctcgca gcggaccggc gtcgcctggc tcgcgaaagc cgacgccgtc
    12781 gccgcgctcg tcgtggcggc catcgtcgtc gtcctctcgc tgaacctcgg gaggaagtcg
    12841 atcgacgacc tcgtcgacgc ggtcccggtc ggcctgaagg aacggctcgt ggcggcggcg
    12901 cgcgtcccgg gcgtcgtcga cgtcccggcg ctccgggtga ggaggacggg gccgcacgcc
    12961 ttcgtcgacg cgacgctgac cgtcggccgc cacgtcgcgt tcgagcgggc gcacgagatc
    13021 gccgacgccg cggaggcgga gctgaagcgg ctcctccccg gcgccgacgt cgtcgcccac
    13081 gtcgagccgg tctccgaggg gaacgagggg ctcctcgcca cgacgcgcct cctcgccgcg
    13141 cggcacgggc tcggcgccca cgcgatccgg acgcacggga cggacgggaa cgtctcgatg
    13201 gagctccacc tggaggtgga cgagcagctg accgtccggg aggcgcacga cgtcgtctcc
    13261 gtgttcgagg aggaccttcg ccgggcggcg cccgaagtcg cgcacgtcgt gagccacatc
    13321 gagccggtcg gagagacgac ggccgtgcgg aagacggcgc ccgcggacga cgccccggtc
    13381 cgctccgagc tcgatcggct gcgagccgag agagggccgt cgttccggcc ggaagggctc
    13441 gcggtccgtc gcggcgagga cgggctcttc gtctccttct cgcttccggt gccgggcgac
    13501 ctgtccgtcg gagaggcgca cgccctctcc gacgaggtcg agcgggagct gaagcgccgg
    13561 ctgggcgggg gccgggtcgt cattcacgtc gagccgcggg aggagtgagc cggcggcgag
    13621 gccgtcggtg tccgccccta cgtccggccg ccgaggtagc tcgtgaacca gcgaacgacc
    13681 tcgaccgtcg cgcggacgcc gttcgaggtc ttccggagcc cgtgaccttc gtcggggaaa
    13741 aggacccatc gcgtcgggac gccgtggcgg tcgaggctcc gtacaagggc ctcggcgccc
    13801 tcgacggggc agatcgtgtc gttcgcgccg tgcaggacca gcgtcggcgt cgtgacccgg
    13861 tcgaggcggc gggccgggga gagcgtcgcg aggaggagcg ggtcggtctc ggggtcgccg
    13921 tactccgcct tcgtggcggc cgccagccac ggctcggtcg tccggaggaa ggaggggagg
    13981 tcggcgaagc cgtggagcgt ggcggcggcc gcgaagcgtc gcgggaacgc ggcgagggcg
    14041 gccatcgcga ggtggccgcc gccggagact ccgaggagcc cgatccgttc cgggtcggcc
    14101 aggcccgcgg cgacggccca gtcggcgcag gccgcgacgt cctcgatcgc gtcgcggcgg
    14161 agcgggccgt cgtcgagact cgcgaatctc ctgccgaagc ccgcgctccc gcggacggag
    14221 ggcgcgagga ccgagatccc ctccgcgacg agcgcgtgga cgtcggcgcg gaaggtgccc
    14281 ggctcggagg tctccgggcc gccctccagg gagaggacga acggccccgg gccggtggcg
    14341 ccgcgggggc ggtagagccg ggcggaaatg gggaggccgt ccctcccggg aagccgcacg
    14401 agggtcggct tcacgagcga gccggggtcg acgccggcgt gggggctcct cgtcacctgg
    14461 cggaacgtcc cgcgagccct ctcgaggacc cagacgtcgg acggcgtcgc gggaccggag
    14521 aggaccagcg cgagccgcga cccgtccggc gagaagacgg gctcggaggc ggtctcggcc
    14581 gggagggccg gtccggggcg gcttcgcagc gtggcgaggt cgacgaagac gagctcgttc
    14641 cgcccggcga cgctccagac gagcgcggcg gtgagcccgg ccccgtcgac ggcgaacgcg
    14701 gagagctccg cgtcgtcgcg ggcggcgacg aactcgaccg ggccgggagc gccgttcggg
    14761 gcgaagcgga tccgggcgaa ggcgaggaga tcgcggccgg cgtcggtgga gagccagacg
    14821 gtgctcgcgt cgggcgagag cgcggcctgc cggacggccg ctgggctccc ggcgttcgtc
    14881 aggaggagct cggccccgct cgcgaggtcg acgagaaaga ggtcgtcgtc gccagcgcgc
    14941 gacgagcggg cgaggagggc gaagcggccg tcgcaggaga cgtcggagaa ggagctctct
    15001 cccgggacgg aggtccgggc cgaggcctct cccgtgagcg ggtcgaggag gtaggcgtcg
    15061 atcgcggcga cgctcctccc gctggaggcg acccggaggc gggaggcgtc gggggtgaat
    15121 ccgtcgaggc ggttcgtctc cttcccgccg cccgtcacga gacggaggcc ggtgccgtcg
    15181 gggcgcgaca cccagacctg ctcgttcgcg gctccgcccg gcgcgaccgc gaacgccagg
    15241 agcgcgctcg cgggtgacca gcggaccctc acgaccgggt cctcgaacgg cgtgacgcgc
    15301 tccgggaagc cgcccgtgag ggggagcgtc caggcctgtg gcacgccgga gaggtccgag
    15361 acgaaggcga ggcgcgtgcc gtcgggcgag aaggacgggg accacgcgcc accgacgcgg
    15421 gccagcgtcg cggcgcggac ctcgagctcg cttccgccgg cgggaagcgc gaggacgaga
    15481 gcgaggagga tcggcggggt ggtcggtcgc atgctcacct cgtgcgggtc tgccggcctg
    15541 gcgtcgtcgt gggatcatcc tgccggaagc gaaagcgatg cccgaagtcc gtcccgtcgt
    15601 cgccgtcacc ctcggcgacc ccgcctcgat cggtccggaa gtcgtcgtga aggccctcgc
    15661 cgggggggag ctgggggccg tctgcgtgcc ggtcgtcgtt ggcgaccggc gggtcgtggc
    15721 gcgagccctc gcgacgacgg gagccgtgct cgaggtcgtc cccgtagagc gtcccggcgc
    15781 ggcgacggga aaggccggtc gggtcgaggt cgtcgacgtc ccggcctacg ggatcgatgc
    15841 catcgactgg ggacgggtcc aggctccggc cgggcgggcc gcatacgagt cgatcgtcgt
    15901 cgcgatcgac ctcgccctcg ccggcgaggt cgatgccgtc gcgacggccc ccgtgagcaa
    15961 ggaggccctg cgcgcggcgg gtgtttccca gatcgggcac acggagatct tcgcggagcg
    16021 gactgggacg ctcgagccgc tcacgctctt cgagacgaga gggctccgaa tcttcttcct
    16081 cacgaggcat ctctcgctcg ccgaggcgct ccggcaggtc acggcggcgc gggtgctcgc
    16141 gaccctgcgc ggcgccgccg cggctctccg gacgctgggt gtcgccgcgc cgcggatcgc
    16201 cgtggcggcg ctgaacccgc acggcggcga gcacgggctc ttcggtgacg aggaggagaa
    16261 ggagctggtc ccggccgtgt tgcgcgcgag ggaggagggg atcgacgcat ccgggcctct
    16321 cccggccgac tccgtcttct ggcacgcggc gcacgggcgc ttcgacgcgg tcctctcgct
    16381 ctaccacgac caggggcacg tcgccgcgaa gatgtacgac ttcgagcgca ccgtctcgat
    16441 gacctgcggc ctgcccttcc tccggacctc cgtcgaccac gggacggcgt tcgacctcgc
    16501 cgggaccggg cgcgcgagcg cgacgagcat ggaggaggcg atccgggcgg cggcgaggca
    16561 cgcgttcgcc gtgcgtggcc ggacggcctg atcaggagcc gcgttcgagc gcgacgacgc
    16621 ggggcgcgag cgcgggagag gcgtggagga agaggccgag cgggacgagg ccggcgcggc
    16681 ggaagaccga cctgtaggct cgcggggagc gccggacgaa gccgtcgacg tccccttcga
    16741 agtcgtcctc ggaggtgaac gcctcgagcc agaggaggcc gcgcgcgagc gacgccatcg
    16801 cggcgaggcc gcgccgcagc tgggcgtcgc ccacgtagtg gaggacgtcg gagcagacga
    16861 cgaggtcgaa cggcccgccg agagagagcc gctcgaggtc gtcgaggccg ccgcgcacga
    16921 cgccgcgcgc ggctccgcgg gccgacgccc aggagctcgg ctcgacgccc acgtagcgga
    16981 ggcgcggccg gaggcggagg agcgcggccc gccacggggc ctcgccgcag ccgacgtcga
    17041 ggacgttccg gagcgggcgc tcgaggaggt gctcggcggc ggcaagggcg agcgcggcct
    17101 tccgccggac gaccgcggcg cgggcggcgc gctcgcgcgg gtcgtggtac cagcgggcga
    17161 agtaggcggc atcgtagagc ttctccggag cgggcgtgcg cggcatgggc gaattctgcg
    17221 cgccccgcgg agcgtccgca ttccgggcgc tccgaacggg tgccgcccgt caccgccggc
    17281 cgggatcgcc gaggatagac tctctccctc gccgcggcgc gagccccggt cccgtgaaag
    17341 gagatttccg tgaagaccat cctccggatt tccctcggtg cgctcctcct cctcccgctc
    17401 gccgcgacgg cgcagtggga gggctctctc gagatgaaga tcagcggcaa ggacgcgaac
    17461 ggcacctcga aggcgctcgt cgggaaggca ggcgcccgga tgcagatcga gatgacgaac
    17521 gcgcagctcc ggcaggcggg ccagggcgcg atgaagatga cgatcctgac ccggacctcc
    17581 gagccggacg tctcctacat gctcaacgac gcgcagaaga gctacgcccg gatcgacgtg
    17641 aaggagatgc aagaggcgat cccggcgtcg gagcgggaga agaagtgggc cgtgaagcgg
    17701 ctcggctccg acagggtcgc cggctacgcc tgcgagaggg ctctcgtcag cgaggagggc
    17761 ggggagaccg agaacgaggt ctgcgtctcg aaggagatcc tcggctcggc cggcctcctc
    17821 agcgccggac gccgggacgc ctcggcgcac gggctcgaga aggcgctcaa ggcgaacggg
    17881 ctcgagggga tgccgatccg catggccatc cggcagcgcg ggaagtccga ggcgacgatg
    17941 acctgggagc tggtcaaggc cgagaagaag gcgctccccg cgtcgacctt cgagatcccg
    18001 gccggctacc gggagacgag catgatgggg acgatgatgt cccccgagca gcagaagcag
    18061 atcgacgacg cgatgaagaa cctgacgccc gagcagcgga agatgttcga gcagatgatg
    18121 aagaagcagg ggggcggcgg ccgctgacgc cgcgccgcct tccgcccggg ccccgcgacg
    18181 ccgccggcag cgccggcggc gttcgcgtct ccaccccgct cagatcccgg gaaggatcgc
    18241 cttcgcgatg accatgtaga tcgcgaggcc ggtcgcgtcg acgagcgtcg tgatgagcgg
    18301 ggcggaggcg acggtcgggt cgaagtcgaa ccgctccgcg aggagcggga cgagggccgc
    18361 gatcgcgttc gcccagacgc agacggccag gatcgtcagc ccgaccgtca gggcgaggct
    18421 cccgccgacg ccccagagag agacgcgccc gacggcgacg gcgcagagga tccctccgag
    18481 gatcagcccg gagctcatct cccggaggca gacccggagc gcgtccttca ccttcacctg
    18541 cttcagggcg agcgcccgga tgatcgtgga gaccgtctgg cttcccgcgt tccccccggt
    18601 gccgatgatg agcgggatga agaacgagag tgcgacgact ttcgagagct cgccctcgaa
    18661 gtgcttcagc acggagccgg tgagcgtccc cgcgatgaac agcagcagga gccacccgag
    18721 ccggctcttg acgacccgcc agaggggcgc gccgaaatac tcgaactcgt gcttctcgtc
    18781 ggggtcgctc ggcgcggcga ccgcgcccat cgcgagggcg gcctcggtct cgcgcgcgac
    18841 gaggacgtcg acgacgtcgt ccaccgtgac gacgccgagc gccttcccgt cttcgccgac
    18901 gaccggcatc gcgttgaagc cgtacttggc gacgaggcgg gcgacctcct cctgcggcgt
    18961 gtccggcgcg acggagatga cgtcgtgcgt catcagctcc cagaccttcc ggccgggctc
    19021 cgcggggaag agcttccgga gggaggtgac cccgacgagc cgtcccttct cgtcgaccgc
    19081 gtagaggtcg gccacggtgg cgacctcggg gtcgatcttc cggaggtggg ccatcgtctc
    19141 ggagacgctc cagtccgggc tcaccttcac gaagcggtcg gtcatcagcc ggccggccgt
    19201 tccgggctcc caggcgagga ggcctcgcgc cgccgcggcg tccttggccg acatccgggc
    19261 gaggaccggg aggcgccttt cctcggggag gaacgagagg agtcgcgccg cgtcgtcggg
    19321 ctcgagggag tcgagggcct gggcgatctt cgcgggcggg agccgggtca gccccatccg
    19381 aaggactccg cctccgaggt acgagaggac gcctccgagc tgctcctcgt ggagctgctc
    19441 gaggacgggg atccggtcct cccgcggaat cgccgcgagg aggtcggcga cgtcggccgg
    19501 gtggaactcg agcagcccct cgcggagcgc cgcggggtcc tcacggacga tctcgaggac
    19561 gtcggggccg aggagctctc cgatccgcac cgtcggcgag tttagtccgg accggccggc
    19621 ctagaatgac cgggagacga cgaggccgag gcggggccgt ggggtcggag gcggggcccg
    19681 cccggaaccg cggaggaacg gatggacgac cgggggcaga cgtgagcgcc gaggacgggc
    19741 gggtcctcgt cgtcgacgac gacccggtga accgcgccat cctcgtcggg agcctcgagg
    19801 cccaggggta cgcggcgggg atggcggccg acggtcgcga ggcgctccgc ctcctccgga
    19861 cggacgggtt cgacgtcgtc ctcctcgacc tcgtcatgcc ggtgatggac ggcttcgagg
    19921 tcctccggac gatcaaggcc gacgagcgtc tccgctccgt ccccgtcatc gtcgtctcgg
    19981 cgctcgagga gatggagagc gtcgtccgct gcatcgagat gggggcggcc gactacctcg
    20041 cgaagccgtt cgacccggtc ctcctccggg cgcgcctccg cgcgtcgctc gcctccaagc
    20101 ggctcgacga cgtcgtggcg gcccacctgc tggagatcga ggagctcgcc gagcagctca
    20161 agacgaggaa ccgcttcatc caggagacgt tcgggcgcta cctctccgag gcggtcgtgg
    20221 ccgagctcct ggaatcgccg gacgggctcc ggctgggggg cgagaagcgg aaggtcacga
    20281 tgctcatgtc ggacctgcgg ggcttctcgg cgatcgccga gcggctcgag ccggaacagg
    20341 tcgtgagggt catcaacaac tacctcggga cgatggccga cgtgatcctc tcctgggacg
    20401 gcacgatcga cgagttcatc ggcgactccg tcctcgggtt cttcggcgcc ccggtcgccc
    20461 gcccggacga cgcccggcgc gcgctcgcct gcgcgctggc gatgcagaag gggatggagg
    20521 acgtgaacgc gaggaaccgg agcgagggcc tccccgccgt cgagatgggg atcgccgtcc
    20581 acacgggaga ggtcgtcgtc gggaacatcg gctcggagaa gcgcgcgaag tacggcgccg
    20641 tggggagcca ggtgaacctc ctcgcgcgga tcgaggcctt cacctgcggt ggccaggtcc
    20701 tgatctcgga gcggacgctg ggagaggccg ggcacgggat cgtgaccggc ctctcgctcg
    20761 cggtccgggc gaagggcttc ccggagccgc tccgggtcca cgacctactc gggctcgtcg
    20821 aggcgccgca ccttcacctc gggcggcgcg cggaggagat gcggccggtc gacccgccgc
    20881 tgaaggcggt cttcacggtg ctcgagggga agcacgtcgc gcgtgtcgag gagccggccg
    20941 aggtcgtcgc gctctcgccg cgcggcgccg cgatcctcac gagcgcgacg gtcgaggtgc
    21001 tctccgacct gaagctccgc atcgtcggtc ccggcgggct cctcccgggc gagctctacg
    21061 ccaaggtcct tccgtcgccc gacggcggac cggtgaggct ccgcttcacg tcggtccccg
    21121 tggagctcga gccccttctc tcgccgtagg ggctcgaccc ccgggaatag aatcgaatcg
    21181 tgccgcgtcc aacgaggcgg gatcgcgcgg ggaccttcgg gctcgtcgtc gggctcggag
    21241 acctcacgct cgagccggtg gacgcgatcg tcaacgccgc caacccgtcg ctcctcggcg
    21301 gcggcggcgt ggacggggcg atccacgcgg ccgcgggccc cggtctcctc gcggtgtgcg
    21361 ccgggatcgg aggctgtcct cccggcgagg cacggatgac cggaggccac cggcttccgg
    21421 cccggcacgt cattcacacc gtcggaccgg tctggcgcgg cggaggcgcg ggagaggacg
    21481 agacgctggc gtgctgctac cgcgcgtcgc tccggctcgc cgaggaggcc ggccttgcga
    21541 gcgtcgcgtt cccgtcgatc gcgacgggga tctacggatt cccgatcgag cgggcgagcc
    21601 ggatcgcgct gagggagatc cgggccttcc tcgcggcgtc gcggtgcgtg aggtcggtga
    21661 aggtcgtcac cttcagcgag agggacctcg cggcgtaccg ggcggcggag aaggagctcg
    21721 cgtcgtgacg gatcgcgccg gccgggcggc ggtcctcgtc gccggggcca ccgggctcgt
    21781 cgggggcgcc ctccttcgac gcctcctcgc gacgcccggc gggccgcggg tcgtggcggt
    21841 cgtccgccgt cccctggagc ccgtctcccc acgccccgac gtggtcgtcg ccgacttcgc
    21901 gcgtctcgag agggtcgacc tcccgcgcgc cggcacggcg ttctgcgcgc tcgggacgac
    21961 gatggcgaag gcgggctcgc gggaggcgtt ccgcgcggtc gacctcgacg ccgtcgtcgc
    22021 gttcgcgcga gcggcgcgcc gggcgggggc gacccgcttc ctcggcgtca gcgccctcgg
    22081 cgccgatccg cgctcccgtg tcttctacaa ccgggtgaaa ggggaggccg aggaggcgct
    22141 gcgcgcgctc gggttcgagg ggctcgcgct cgtccggccg tcgctcctcc tgggggaacg
    22201 gcccgagacg cgtccggcgg agcgggcggc cgcggccgtc acgcgcatcg tcccgtggaa
    22261 cctcgccgga ccgctcgcga agtggcgacc cgttccggcc gacgcggtcg cggcggctct
    22321 cctcgcgatc gccgggcagc cgcttcgggg cgtccgcgtc gtcgagaacg aggagatcca
    22381 caggatcgcg tcctcggatc cgctcagcgt tcgatagaat cgaccgcatg tcgagcgaca
    22441 ctccgggctt ccctccggct ccgcccctgc ctcctccgcc catgccgccc ccgcctgccg
    22501 gaggaggctg gcagacgccc ccgcccggcg gcccggcgct cgccggcctc ccgtgggagc
    22561 accgcgccga gctcgggttc ctgaacgcct tcgtcgagac gatcaagctg ttcgtcacga
    22621 acccggccga cgcctggcgg cggacgaagg agaagggcga cttcgccgag ccgctcatct
    22681 tcgcgatcct cgtctcgtgg atcggcatcg ccgtctccac gctctggagc ggcctcttcg
    22741 gccaggcgtg gatcagcttc ctgccgccgg agctgaagga gaagatggcc ggggcgttcg
    22801 cgacgaacgc ggcggcgctg atgatccagg tgggcctcgc tccgatcttc gtcctgatcg
    22861 gcctcttcgt cggcgcggcg ttctaccacc tctccttcat gatcgtcggc gccacgaagg
    22921 actcgacgtc cggcttcgag gggacgttcc gcgtcgtctc ctacgcgtcc gtctcgcagc
    22981 tcgcgaacgt catcccgttc gtcggggggc tcgcggcggt cgtctggaac ctcgtcctcg
    23041 tcgtgctggg cgtcgtccgg atgcaccgga cgacccaggg gaaggcgatc gccggggtcc
    23101 tgatcccgct cctcctctgc tgcgtctgca tcgtcgtcgt cgtcgcgctc gtcatcggcg
    23161 gcctcgcggc ggccttcgcg agccgctgat ccggtgacgg acgtcgcgcg cggggcggcc
    23221 ggcgccggcc gcccccccgc cgtccagctc gggctcctct ggggggcggc cgccgtctcg
    23281 gcggtcgccc tcgccgtctc gttccgcggc tccgtcgcga cgtcgctggc gctccttccg
    23341 ccgtgcccgt tcaagtccgt caccggcctt ccgtgccccg cgtgcggcag cggccgggct
    23401 tcggccgccc tcgccacgct cgacctcccc tcggccctcg cctcgaaccc gctcttcacg
    23461 ttcggcgcgt tcgtgttcct cctcggcggt ctcttcgcgg ccgccgccgc gttcacgggg
    23521 agaggcgtcc ccgagccgcg gacgcttccg gttcccgtcc gcgccgctgt cgtcggcgtc
    23581 gtcgccgcga actggctctg gctcctcctc gacggacggt gacggagcga tgcctcgcgc
    23641 gtcggccgac cccgccaccg acttccggat gctctgcgac gcgctgttcg agttcgtcgc
    23701 ccggcatccc caggccggac cgaagatggc ggacctcggc atcagtggcc gcggggtctt
    23761 ccccgacctg aagctcgtcc tgaacgtggc gcccgcgggc gcgaagctcc gcgcccgcgg
    23821 ggtgcacgtc acgtggagct ggggcgcggc accgaagggg ttcgctccga aggtcctcgt
    23881 ctcctgcccg acggcggtcg cgaaccgcct cttccagggg aaggagaacc cggccctcgc
    23941 cctcgacagg ggcgacctga cgctctcggc cctcgacccg tcagtgaacg agaggaggct
    24001 tctaaatctc ctcccggtcc tcgtccccct ccggaccgag gtcccgggcg tcctccggaa
    24061 ggccggcctc gcgcggctga tcctgtagcg ccgcgcgctc ggccgcctcc cactccgcga
    24121 ggcgcgcgtg ccgccgcccg cgcgcgacgc gaacggccca gagcgtgagg aggacgatcg
    24181 cggtccagac ggccgccgtc gacgtcacga tcggaagcca ccgttcgagg ctccttctcg
    24241 acccccagaa cgcccccgat tcgtcccgga aggggactcc cgtggcgctc cggaacgcct
    24301 cctcgaacag ctcgcctccc gccatccggc ggaggacccg cttcgggaag gccgacccgt
    24361 gacgggcgag gaggtctccg aagaacgcgg aggagagggc gtaggcgcgg ttcgcgtcgc
    24421 tccgtccgcc gaggaagagg gcatcgacct ccgcgaatcg aagacggggt ccggtcagga
    24481 ccgcgagcgc ctcccggagg tcgtcctcga tcacccgccg gcgtccggcc gccatcgcga
    24541 gcccctcgtc gaaccagcgc gggacctgcc gtccccccgc ggcgcgaaac gtcaggacgt
    24601 gcgccacctc gtgccggacg acgtcgggga ggccatcgtc gggccaggag ggcgtccgcg
    24661 tcgggatcag gacgaccgtc ccctcggccg gagcggcgta cgcgacggcc cacgaggggg
    24721 cccgggtcgc gagcgcgctc ccttcggcgg tcacgacgac ccggacgtcg cccggctcga
    24781 agaggccggt cagctccatc gccggcagga aagccacggg gtcgaggcgc tccacctcgc
    24841 gggcgtacgc ggcgagggag gggggcgcct cgacgacgag gcgaggcggg gccgaaacgg
    24901 cgacggcggc cccgaggagg gccgccgtcc cgaccaggaa ccgtgctgcg attcgcgccg
    24961 tcagcgcttc cacttcttca ggatgtcgac gacgttgtcg ccgatccgga ggaggttctc
    25021 cttggaggaa ccgccgatgt ggggcgtcat gacgacgttc ggggcggtga ggagggggca
    25081 ggacgggtcg ggcggatcgc tgtagaagac gtcggtcgcg taggcccgca ccttcccgct
    25141 cttcagcgcc tcggcgaggt cctcctccac cacgaccttg ccgcgcgccg tgttgatgag
    25201 gatgacgccg tccttcatct tcgcgaaggc gttcttgtcg agcatcccct tcgtctcggg
    25261 cgtgagcggg aggtggatcg agatgtagtc ggagagcgcg aagagctcgt cgtcgtcctc
    25321 caccatgtcg gcgagggcgt ggttcgagat gtagtggtcg tgcgcgacga cccgcatccc
    25381 gaaggcgatc gcgcggcggg cgacctcggt cccgatgagg ccggcgccga ggaggccgag
    25441 ggtcttgccg tagagctcgg tccgcttcag ctccttcttg aggaacttcc cctccttgat
    25501 gccgttgtgc ccctccagga cgcggttcgg gaccgtcagc atcagggcca tcgcgagctc
    25561 ggcgacggcg atggacgagg cgcgcggggt gttcttgacc tcgatcccct tctcgcgggc
    25621 ggcctggacg tcgatgttgt cgaggccgac gccgccgcgg atgatcagct tcaggttcgg
    25681 tgcgccctcg aggtactcct tcgtgacctt cgtcttggag cggacgagaa ggacgtcggc
    25741 ttcggcggtc ttcgccgcgt cctgcgtcac ctctccgaac ggcgcgagac ggccggggag
    25801 ggagtcgtcg aaggcgtcgg cgatcaggat cttcatctct tcctcctggt ggatctgggc
    25861 gccggggcgc ccagcggcca ttctaggacg gaagggtccc gacgacggcg acggggaagg
    25921 cggtcggggc cgcgcggagg aaattggccg gtcgccggct cactcctccg tggcggcggt
    25981 gtcgatcatt ccgggtgcga ggtacttccg cgtctcggcg gcgaggacgc cgctcagcgc
    26041 gaggagcgag atgaggttcg ggatctggag ccgttgcggg ctcgcggggc tcgcccgcga
    26101 cggctccgcc cgagcctcac tcctccgtgg cggcggtgtc gatcattccg ggtgcgaggt
    26161 acttccgcgt ctcggcggcg aggacgccgc tcagcgcgag gagcgagatg aggttcggga
    26221 tcgccatgag gccgttcgcg atgtcggcga aggaccagac gacggggagg gtcgcgacgg
    26281 agccgacgag gacggcgacg acccagagga ggcggtaggg gcggacgacc ttcgggccga
    26341 agaggtactc cgcggccttc tcgccgtagt acgaccagcc gaggatcgtc gagaagacga
    26401 acgtcaggag gccgaccgtc aggaccgccg ggccgacgtg cgggatgtgg ctgaaggcgg
    26461 tcttcgtgag cgcggcgccc gcgagcccct cgtgccagtc gcccgagttc acgacgacga
    26521 ggccggtcat cgcgcagacg acgacggtgt cccagaacgt ccccgtggag gagacgagcg
    26581 cctgccgcac ggggttcttc gtcatcgcgg cggcggcgac ga
//
LOCUS       DAOONL010000043        26446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1678_length_26446_cov_18.735781, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000043 DAOONL010000000
VERSION     DAOONL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26446
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_26446_cov_18.735781"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLP50_08345"
     CDS             complement(<1..409)
                     /locus_tag="PLP50_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51598.1"
                     /translation="MSENPEVDSPETSVPVPPDPSRADQARKGLLVFFALAALGSGVF
                     QGLILRSGKPIGESPWLVYGLMWTPGIASLVARLVFREGFRDVSFRIGGATAWKAIGL
                     AWLLPPIVGFVAYGAAWATGLAPFEIPALAEVGL"
     gene            512..2206
                     /locus_tag="PLP50_08350"
     CDS             512..2206
                     /locus_tag="PLP50_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA51599.1"
                     /translation="MRRTFVPLPGRSAGRRGILLLALLLSTSAAHARPQAAEKAPPRI
                     RASVDVTVLDLDFVATKDGRPVHDLRRDELVVKVGGRVLPVDHFARVEASRISAPSAA
                     AAETPPPGRAPEAGTVVPRQLLVYFDDARLQPWQRRPVIDGLEGLLARLGPADEASIV
                     SYNGGMLVLAPFTNDRARLADGLARLRDLVPRGFHRAHLFRNAAAAMETEDRIQRNSS
                     GRSRPTSLKDSILRTWVEEERTRTRAGLAELRRTVSALAARPGKRRMVLVTTGWERMP
                     GQTLAQLGFGRDTWRQFDTSTWKETEAILRAANRSGVTIDVVDAKGFESELDIELSEM
                     REVGKWIARLNTRDGMATIAETTGGAFVENVSFYERALGEIWRESSDYYSVGVTLPEA
                     LPAGKVLKVHLSCSRPGVTLKTRSSVGARSAAEEARDLAESALLAPPPPSAFAIRLEL
                     GAPRPDGVFSRRRVTPWRLFVSGGDLTFSPDGDRRTAVVEVAFAAIDESGGRSEVAPE
                     RFDVSVPAARLAETAGRDLPLSGLLRTRTGRHRIVATVRDVASGRTAVATAPLVDD"
     gene            complement(2278..2730)
                     /locus_tag="PLP50_08355"
     CDS             complement(2278..2730)
                     /locus_tag="PLP50_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51600.1"
                     /translation="MERDFVEMLSALDAAGAEYLVVGAHALAAHGVPRATGDLDIWVR
                     ATPENARRVLAALREFGASLFDLTADDLSSPGTVFQIGVVPVRIDLLTSISGVGFEEA
                     WAGRVEVPIEGVTVGVLGRRELVRNKRATGRPKDLLDAETLAAAIAEG"
     gene            complement(2678..2896)
                     /locus_tag="PLP50_08360"
     CDS             complement(2678..2896)
                     /locus_tag="PLP50_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51601.1"
                     /translation="MSGRAERSSWPVKRIALAEEGGMPDPVVAAMTPSERIALVERLT
                     RNAWAFKGERIDGARLRRDVVRTRRGGG"
     gene            complement(2991..5039)
                     /locus_tag="PLP50_08365"
     CDS             complement(2991..5039)
                     /locus_tag="PLP50_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPA51602.1"
                     /translation="MSEKPFQPFVPASSTMTEFTGRALVLGLLMAVVLGAANAYLGLK
                     AGMTIAATYPAAVIGMAVLRIMKGSLLEENFARTVGSIGESVAAGAIFTIPAFVILGM
                     WKFEGFNAKEYGIATALMILGGLLGILFVTILRRVMVEDKGLPFPESVAASEIHKAGQ
                     RGADAAIQLFRAMGVGAVIKLLGDLGVFRASNDFHVAVGKVKESFVRLGLSKDSDSIA
                     AGGVTTISAPAATPAYMGVGYIIGPELGALNFAGGLLAWGLFVPLLVFFLGPNLIERY
                     TDASSGAQDWAGLAGHLYRYIVRPIAVGGMLVGACFTLWKMRKNLISGIKRGVADVKK
                     SAAAAEATERTEKDLSFKVVLLGIAAVFVLMVALYFYFTNVLGGALLAAIVMLITGFF
                     FAAVSGNLVGLIGSSNNPISGLTLATTIVAALTMVVIGVKGVEGVAAVLAVAAVVCVS
                     SAVAGEMLQDLKVGHILGGTPWKMQVGDVIGVVIAGAVMFFPLYLLHTSDLASNPGVG
                     GFGGKNLPAPQAGLMAALSQGIVGGEMAWPLVLVGIAMGITLILVRVKSPMLFSVGMY
                     LPLETTFAIFIGGLIRGFVDKLAGKRDFNVAQKARVENAGVLAASGLIAGEALVGLLV
                     AGVVAFRSDNKFPQLEAFAGIAPWLAIPVVALLAAYLIYVPLGKAGAADEPAPPTAVM
                     "
     gene            complement(5239..5607)
                     /locus_tag="PLP50_08370"
     CDS             complement(5239..5607)
                     /locus_tag="PLP50_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPA51603.1"
                     /translation="MKRLLSLLLLAALPALAAQPSLNVYFQSTLKDPAYQKKVFQKVA
                     STWQAPASFPKVGKKCVVQAVISRDGRVQNAFVSMESGVKAWDKASLAAVEKASPFPP
                     LPSTYEHKTLEAHFHVAVVP"
     gene            5672..7507
                     /locus_tag="PLP50_08375"
     CDS             5672..7507
                     /locus_tag="PLP50_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPA51604.1"
                     /translation="MRPVLASARTSLLPVLAIAAALLPTAPLAAQERPVVPIPPDHWT
                     QGELDNFRSTPSYDRTLAFLRRLEQTSPYLSLSFYGTSAEGRPMPVVVASKEKAFTPE
                     AAAKSGKPVVLVLNGIHSGEIDGKDACLILLRDMALGNRPDLLDTMTLLVVPIYNVDG
                     HERASKWARPNQDGPVEGMGFRTTAQGLDLNRDFLKADAPETRALLSLVAAWDPDLFV
                     DDHVTDGSDAQVTVTVSYGGEPATPEPLRSWLETVMRPALAEVEEKGWKTSPYIEWVD
                     PLDPHKGIDPGPTEPRYGTCYMPLRSIPSVLVEMHALKPYGERVRANAAFLSALLSRV
                     GRDPAPLKTARAAAKGTAKGARPGTPFVLAGETDLSRPETIDFPAYAWEQVVSPVTGR
                     PRLVYDPKTPMTVPIPYYRHTKATLTAPRPAAYLVPAGWPGIEEKLAAHGLRFTKLAA
                     PRTLPVGTYRASEAKFAAASYQGRVRLTAKIARAVETRTVPAGSLYVPLDDPLAPVAM
                     HLLEPEGPDSLFAWGLMSSALETKEYIDPRVLEPLAAKMLAEDPKLAAEWKALLSDPK
                     LAADARARHRFFYTRTPYWDESVGLLPVFRLDAPLEATAPAAPAP"
     gene            complement(7584..8306)
                     /locus_tag="PLP50_08380"
     CDS             complement(7584..8306)
                     /locus_tag="PLP50_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51605.1"
                     /translation="MALHAAIQNLLGAAGVTNVMPGWAILENARLLPPTPGSGAAFGD
                     APAQALRKVMKAYYEYGRAHWTWSQSAGVAAANGGLVKGQIQAVACGSFNQNFKWLAE
                     KGLGINGITNGQEPGQFLTMPGSVCIDSKWVGNVRTSTKTFGALKCFKFAGHYWVAYG
                     GVNYDVCYDNTFHAVSEIIWTRLLPPDQSLVGKGGLTATQIYRLEKPLPEGDHLVMLQ
                     QNGPNGWPSWQIVPKAQIKSLK"
     gene            complement(8444..9025)
                     /locus_tag="PLP50_08385"
     CDS             complement(8444..9025)
                     /locus_tag="PLP50_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPA51606.1"
                     /translation="MRNVCVYCGSSEGRDPAYAALARALGRALVARGLGLVYGGASVG
                     IMGIVADTVLGLGGRVTGVMPEALVRKEIAYGGLTELVVTSSMHERKTRMAELSDAFV
                     ALPGGIGTLEEIFEAWTWGQLGLHAKPCGFLSAAGYWDGLVGFLDHAVRERFVREAHR
                     AMLVVAEDPEELLDRFAAFRAPEVPKWIRKGDE"
     gene            complement(9022..9522)
                     /locus_tag="PLP50_08390"
     CDS             complement(9022..9522)
                     /locus_tag="PLP50_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPA51607.1"
                     /translation="MGGRPSTFDPARTYAFLEDEGGAVVLPGGAEFWSALAAGPPFPP
                     DVGRVVSGSGWLAALYALDVDGAAWERHPNGAELLLMVTGEMDLVLEGPGGEVTVRMP
                     AGRAFVVPRGAWHRQVVRVPGTYVGVTFGKGSEHRPLGEVAARGGRRAPPGRSRPAPS
                     RRPAKR"
     gene            complement(9522..9893)
                     /locus_tag="PLP50_08395"
     CDS             complement(9522..9893)
                     /locus_tag="PLP50_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA51608.1"
                     /translation="MQLGTFSVSLAVKDLDASLEFYEKLGFEVFAGQADQKWLILRNG
                     DVVIGLFQGMFERNILTFNPGWDVNARKLDSFVDVREIQKALKARGVTFAQEADESTA
                     GPASFVVVDPDGNPILFDQHV"
     gene            complement(10001..10513)
                     /locus_tag="PLP50_08400"
     CDS             complement(10001..10513)
                     /locus_tag="PLP50_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51609.1"
                     /translation="MRVSARSRLMPAVALACGLFASSAAASEFRDAQFGFSLAPPRFG
                     APAEGTSLVRLVVTGPPDGGFSPSVNVTVQETRTTREAFVRLSESQFQEAGLKLHSKE
                     NRTVSGRPAVLLDYEGSISGPRLRFLALAVILPDRVLLATCTGLASTYPSLEPSFRQS
                     LDSFKLTPSP"
     gene            complement(10591..11130)
                     /locus_tag="PLP50_08405"
     CDS             complement(10591..11130)
                     /locus_tag="PLP50_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51610.1"
                     /translation="MTTRDSAIDDSRRSSPRAAPARPARIRSAEPGDVPGWLRLAREV
                     EHLFGPMSEVPEFRAALETAIDGGHALAAVAPSEDGAEELAGGIVFSPEGGAIEWLAV
                     STRARGAGLGRELLAAAIARLDPARPVSVQTFAPASADGAAARRLYASFGFEDREDAE
                     ATPAGVPTVIMVRRASAGR"
     gene            complement(11127..11987)
                     /locus_tag="PLP50_08410"
     CDS             complement(11127..11987)
                     /locus_tag="PLP50_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPA51611.1"
                     /translation="MNASVLKLGLLAGLVVAAPGGSEVRAPAVARERVADLRRFFAGG
                     APVEAAFVLRNGRTGEVVRHGPARAAERLLPASTFKVPHTLIALETGVVSDADFVIPF
                     DESRKRDGFWSDDWSRDQTLRSAFQRSVYWYYRETARRIGPKRMAESLKRFGYGNESL
                     AGGVDAFWLEGGLRISADEQVLFLQRMNEGRLGVSDRSLEVLRSIMVLERGPGYTLRG
                     KTGTADVTPTRELAWLVGFVERADETWYFALNLEGEEVWERWGPGAARRQLVSSLLRE
                     LGVLPAAGAK"
     gene            complement(12001..12360)
                     /locus_tag="PLP50_08415"
     CDS             complement(12001..12360)
                     /locus_tag="PLP50_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPA51612.1"
                     /translation="MNVVNLKERLSRFSTYWDPKIVGELNGQHVKLAKLKGPFVWHSH
                     EAEDELFLVLDGSFTMEFRDRSVRLCEGEMLIVPRGVEHRPVAEEEVHVLLFEPASTV
                     NTGNAGGPLTVPEPERY"
     gene            complement(12540..13577)
                     /locus_tag="PLP50_08420"
     CDS             complement(12540..13577)
                     /locus_tag="PLP50_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA51613.1"
                     /translation="MRGRTERSGPDPLRVSLVALPDAVISTLGGLFDVLNAFARMDAL
                     GAGAGARPPFRVEIVGEARGSLGLASGVPIDVQRAVSEIDATDLVIVPSVLLGPGGWA
                     TGRSPRLVEWLRAMHEGGAVLCSACSGIFLLAETGLFDGRDATVHFAYARAFTATFPA
                     VPVHPERVLVVSGRREELVSSGASMAWHDLVLYLIARYAGATAAQEVARLFALQWHQD
                     GLTPYLVFEGRRDHGDAEIRSAQEWLDGHFSVANPVEAMVERSRLAERTFKRRFTEAT
                     GLAPIAYVQRLRIESAKRRLERSDTPIEKLAWQVGYEDPAFFRRLFRRTTGLAPGAYR
                     RRFRIPEFARP"
     gene            13758..14825
                     /locus_tag="PLP50_08425"
     CDS             13758..14825
                     /locus_tag="PLP50_08425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51614.1"
                     /translation="MSSPAIDTTRLESLLARAVGDLSAGHGGVMVSIGTKLGLYKALA
                     GAGPLSPRELAERTGCAERYVSEWLNAQVAGGYVDYHAVTRTYELSPEQEMVLADEES
                     PVFMPHAWQVTATMWADEEKTLEAFRTGNGVAWGDHDERLYCGVASFYRNAYRGSLVS
                     QWLPALEGVVARLEAGIAVADVGCGHGHSTLLMAEAFPESRFRGFDVHPASVEAARRN
                     AAEAGLDGRVSFDVAPASGFPGGSYGLICFFDALHDMGDPLGAARQAASALAPDGTVM
                     LIEPFANDRLEDNLSPVARLYYSASASLCCANAIADGGRLVLGAQAGEARLADVFRKA
                     GFTRFRRASETPFNLILEARR"
     gene            complement(14835..15374)
                     /locus_tag="PLP50_08430"
     CDS             complement(14835..15374)
                     /locus_tag="PLP50_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPA51615.1"
                     /translation="MRASVFVGTSVDGFIARPGGTFDFLSAGGSGEEPHGFEEFFATV
                     DALVMGRNTYEVVLPFPDWPYGAKPVFVLSHRPLPPGRAGAVVERMAGEPAEVFSQLA
                     ARGFGHVYVDGGRTIQAFLRAGLIQRLVLSRVPVLIGEGIPLFGPLDADVLLTHVATR
                     TFAGGLVQSEYEVAPAGGK"
     gene            complement(15394..16191)
                     /locus_tag="PLP50_08435"
     CDS             complement(15394..16191)
                     /locus_tag="PLP50_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPA51616.1"
                     /translation="MSTVRIALANVRIPATPEESVLQATAAVAEAGRRGALVICFPEC
                     FVPGYRWPGTTLPPPDPAFLERAWADVAKAAGAAGVTVILGTERVTDRGLQITACVIG
                     PDGRVVGWQDKGQLDPSEEGTYPAFATERRVFTAGELTFGVVICHEGWRYPETVRWAV
                     RRGAQVVFHPHAHVAEPGSYRPVAFADPANTFHEKAVLCRAAENTCYFASVNCASEGS
                     GTTSAIARPDGTLLCHQPYGREGLLVADLDLGAATGLLASRCRTSPI"
     gene            complement(16314..17024)
                     /gene="lepB"
                     /locus_tag="PLP50_08440"
     CDS             complement(16314..17024)
                     /gene="lepB"
                     /locus_tag="PLP50_08440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPA51617.1"
                     /translation="MTPTSKRKALLRDVRQLLLVAGLILTGRSVLADWYVVPTGSMKP
                     TILEGDRVFVLKAAYQVRVPFTSVRLFSTVEPDRGDVVVLRNPDGGRVPFVKRLVGLP
                     GDTVELRDGILLVNGKEQPVRYLPPVEDAAGQTVLLGTERLHGAPHPVRAVPALASAA
                     RTWGPVRVPEGEVLVMGDNRDESRDGRYFGTRPVADLLGRAVGVLWSWRSPWSHGPRL
                     GRFGRPFLDEVDDRTPAG"
     gene            complement(17096..17854)
                     /gene="map"
                     /locus_tag="PLP50_08445"
     CDS             complement(17096..17854)
                     /gene="map"
                     /locus_tag="PLP50_08445"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPA51618.1"
                     /translation="MTVENERDVEGILNAGRVVARVRDEMLERVEPGMTTAELDEIGG
                     RLLERLGARSAPRVTYGFPGSTCISVNEEAAHGIPGPRVIRAGDVVNVDVSAELEGYF
                     ADTGGTTVVPPVSDANGSLCHATRVALDSALSEARAGAPLNRIGRAVERVARAHGFRV
                     IRNLAGHGVGRKLHEEPEGIVSYYDRGDGRRLRLGQVIAIEPFLSTRSAHVTEAEDGW
                     TLVGHPESRSAQFEHTIIVTNGEPIVATRSERSS"
     gene            complement(17932..18213)
                     /locus_tag="PLP50_08450"
     CDS             complement(17932..18213)
                     /locus_tag="PLP50_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51619.1"
                     /translation="MISSSLIHAYAFVAPFAFALFWVAAAVVHVAFAVAVFRHASTRR
                     AEGGSHVFVGPELWTLAALVSGPVAVVTYWLLHCSRLAGSEEPLAPPPA"
     gene            complement(18278..18646)
                     /locus_tag="PLP50_08455"
     CDS             complement(18278..18646)
                     /locus_tag="PLP50_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51620.1"
                     /translation="MTPSPTAAFGDAPGSDLRRTPWYLREVPSPVAAVVVSPLVLVAH
                     VAGLVLGAVFLAALAVLLVAPGLGFVYLLIPDAAFGPRPGHLALAAVLGAALWSLVFI
                     TMTRVVRWSTLACSAKRLRS"
     gene            complement(18769..19287)
                     /locus_tag="PLP50_08460"
     CDS             complement(18769..19287)
                     /locus_tag="PLP50_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51621.1"
                     /translation="MKLDLQPTLSGILLELRPLREEDWADLYAVASDPLIWEQHPEST
                     RHEEEVFRRFFAGALESGGALVAIDRSSGRIVGSSRFHGYDPERREVEIGWTFLSRSH
                     WGGRHNAEMKRLMLDHAFRFVDTVLFLVGPQNHRSRRAVEKIGGALSGTRFERGHEAV
                     EYRITREAWFAD"
     gene            complement(19305..19784)
                     /locus_tag="PLP50_08465"
     CDS             complement(19305..19784)
                     /locus_tag="PLP50_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA51622.1"
                     /translation="MVPKNTICLWYDGAALEAAQFYARTFPDSAVGKVYRAPADYPSG
                     KEGDVLTVEFTVIGIPCLGLNGGPAFRPSEAFSFQVATDDQAETDRLWNAIVENGGQE
                     SACGWCKDRWGLSWQITPRALTAAIADPDPAVARRAFEAMMQMRKIDIAAIEAARRG"
     gene            complement(19787..20410)
                     /locus_tag="PLP50_08470"
     CDS             complement(19787..20410)
                     /locus_tag="PLP50_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPA51623.1"
                     /translation="MREIDGLKSVVRQSPLLDRSRRENSAEHSWHLALYALVLHDLAA
                     GPVDASRVIRMLLVHDLVEVDAGDTPLHSGVPPREQAEREEAAAERIFGILPRPEGEE
                     LLALWREFEAAETADAAFAKALDRVQPLIANVATGGGTWLASGLTRDQVLARYGPTIE
                     KGSPRLWAACEALVRSHFSGRPDASDETGAGVGADPSATDPRHHEGE"
     gene            complement(20549..20950)
                     /locus_tag="PLP50_08475"
                     /pseudo
     CDS             complement(20549..20950)
                     /locus_tag="PLP50_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
     gene            complement(20979..21524)
                     /locus_tag="PLP50_08480"
     CDS             complement(20979..21524)
                     /locus_tag="PLP50_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPA51624.1"
                     /translation="MKTSERAELLHVLKGRFEKHGNRHPGLAWAKVLARLEGNLPALR
                     SLRAMESSGGEPDVLARGGETGPITFYDCSAESPAGRRSLCFDGEARKARKEHRPESS
                     AVEMAAAMGIDLLTEEEYRTLQGFGEFDTKTSSWIATPADVRALGGALFGDRRYGRVF
                     AYHNGAQSYYAARGFRGLLRV"
     gene            complement(21668..22027)
                     /locus_tag="PLP50_08485"
     CDS             complement(21668..22027)
                     /locus_tag="PLP50_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51625.1"
                     /translation="MNARPILASALIAAGLLATACSDTPTEPSSPVANGNLQIQLTQP
                     CALPGSVAVSANGTQLGTLAVPGLSTFSVPAGSYSLAFVRGQDVFAGPGPVQVPAGGT
                     VVVTDPAGACMATTGAP"
     gene            complement(22078..22659)
                     /locus_tag="PLP50_08490"
     CDS             complement(22078..22659)
                     /locus_tag="PLP50_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPA51626.1"
                     /translation="MIITETFEPRSRQDWRKWLGRHHAESKAIWVVFLKKHTGRAALT
                     YQDALEEALCFGWIDGVRKRIDDLRFAQRFTPRTKSSRWSETNRALAADLAARGLMTP
                     AGLAARGSAPADTPPAREPTSGVPEWLAGALRTSPLAWKSFNALPPSHQRRYVGWISS
                     AKRDETRNKRVAEAIALLEENKRLGLGPGEVRK"
     gene            complement(22694..23215)
                     /locus_tag="PLP50_08495"
     CDS             complement(22694..23215)
                     /locus_tag="PLP50_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51627.1"
                     /translation="MRSRLSIALLSAAAVVLPSQGCVSTVFRPSPDRNTVACPPVEPS
                     SVRVLSRPPEEPFTVLGEVEAYVSGYYSDEEIVSRLRAKAAQKGAHAIYFARDVSMET
                     AEKRTTGEYNTPLNDRTWHKRASLVFTAVLLSDAPEPCPGVGRPAPVPGGATTAVTAA
                     TDIPPGAPRSPGR"
     gene            complement(23321..23788)
                     /locus_tag="PLP50_08500"
     CDS             complement(23321..23788)
                     /locus_tag="PLP50_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51628.1"
                     /translation="MSLRIQEVPAAEAPMELLLLADPSEAKIRSYLPESRCFVAFRGA
                     VVVGACVVQPRGAGAHELMCIAVLPEHQKSGYGTALLKWVVELFRGSGARQLEVGTGT
                     FGYQLAFYQRQGFRVTSIDHDFFVRSYPEPIFENGIQLFDMLRLTLRYPGDVT"
     gene            complement(23863..24222)
                     /locus_tag="PLP50_08505"
     CDS             complement(23863..24222)
                     /locus_tag="PLP50_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA51629.1"
                     /translation="MLNRLIPMLPVRHVPASVAFYGKLGFTVETRHEEWGWAMLRLGE
                     CRLMVDQSINVHPDAPRDPVLYLYPDDILEYHEAVRRNGLEIPDLEVTFYGMTEFRID
                     DPDGNRLWIGQNVSGTE"
     gene            complement(24314..24769)
                     /locus_tag="PLP50_08510"
     CDS             complement(24314..24769)
                     /locus_tag="PLP50_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51630.1"
                     /translation="MNEPPRFVTIRHDFNLFEAQLTCATLEAAGIAVLMQNESFAGTV
                     WTEAIAVGGVQVLVPEGSVAQAEAILGPSAHVLADDGAEVHLPEPSASSTPHFRSSCP
                     SGASAGLELFVPLRRQAAALSIVGLPLVPPLKRYRCKACGDVFRRHRHA"
     gene            24839..25276
                     /locus_tag="PLP50_08515"
     CDS             24839..25276
                     /locus_tag="PLP50_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51631.1"
                     /translation="MSTVPVIVGTALAPVIGVLGRLSGLDRDRAMYPVALIVIAAYYV
                     LFATMGGEEALPAELIGATVFAVIAIIGFRTSLWWVAAGIAGHGVFDFVVHPQLIVNP
                     GMPAFWPAFCGSIDLALGAILAVLLLRRAIPARDGAPTPRAHE"
     gene            complement(25277..26254)
                     /locus_tag="PLP50_08520"
     CDS             complement(25277..26254)
                     /locus_tag="PLP50_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPA51632.1"
                     /translation="MTAIVQDRYGSADVLQLREIERPRPKPDEVVVRVHAAGIDFGVW
                     HLMEGLPYAVRLAAGLRRPRNPVRGLELAGVVERVGAEVTAFAPGDAVFGIGDGSFAE
                     YTRASPSKLVPKPSKVGFEEAAVVPVSATTALVGLRAAEIEAGQTVLITGAGGGVGSC
                     AVQIARAMGAEVTGVCSTSKLDFVRSLGAADAIDYTREDITTGARTYDAIIDLAGRRR
                     VAALRRALAPTGTLVILGGEGGGKWLGMGRQAWSRVIGVTARQRFRSPLAFVNQEGLR
                     VLKDMLETGKLRPVVDRRYPLSEAPAAVRALAAGHSRGKSVITIRRAGS"
BASE COUNT         3867 a         9737 c         9219 g         3623 t
ORIGIN      
        1 aaaggcccac ctccgcgagc gccgggatct cgaacggggc gaggcccgtc gcccaagcgg
       61 cgccgtaagc gacgaagcca acgatcggcg gaaggagcca cgcgaggccg atcgccttcc
      121 aggccgtggc accgccgatc cggaacgaga cgtcgcggaa gccctcgcgg aagacgagcc
      181 gcgcgacgag cgaggcgatc cccggcgtcc acatcaggcc gtagacgagc cacgggctct
      241 ccccgatcgg cttccccgac cggaggatca gcccctggaa gacgccgctc ccgagcgcgg
      301 cgagcgcgaa gaagacgagg agccccttcc gagcctgatc ggcgcgggac ggatcgggcg
      361 gcacggggac ggacgtctcg ggcgaatcca cttccgggtt ctcgctcacc cggcgagtct
      421 cccacgacgc gggacgcccg gacatcgacc tcacgcgagg acggaccgtc cggcacgtcg
      481 cgtgctagaa ccttccggac aggagacgcc gatgcgaagg accttcgtcc cgcttccggg
      541 gcgctccgcc gggcgccgcg gcattctcct cctcgcgctg ctcctctcga cctccgccgc
      601 gcacgcccgt ccacaggccg cggagaaagc cccacctcgc atccgcgcct cggtcgacgt
      661 gacggtcctc gacctcgact tcgtcgcgac gaaggacggc cgccccgtcc acgacctccg
      721 gcgggacgag ctggtcgtga aggtcggagg gcgcgtcctc ccggtcgacc acttcgcccg
      781 cgtggaggcc tcacggatct ccgcaccgtc cgccgccgcc gcggagaccc cgccgccggg
      841 acgagcgccc gaggcgggca cggtcgtccc gcgccagctc ctcgtctact tcgacgacgc
      901 gcggcttcag ccgtggcagc gccggccggt catcgacggg ctcgagggcc tcctcgcgcg
      961 cctcggcccc gccgacgagg cctcgatcgt ctcctacaac ggcgggatgc tcgtgctcgc
     1021 gccgttcacg aacgaccgcg cccggctcgc ggacggcctc gcccgccttc gcgatctcgt
     1081 cccacgcggc ttccaccggg cgcacctctt ccggaacgcc gccgcggcca tggaaacgga
     1141 agaccggatc cagaggaact cttccggcag gagccggccg acgagcctga aggactccat
     1201 cctccgcacc tgggtcgagg aggagcggac gcggacgcgc gccggcctcg cggagctccg
     1261 gcgcaccgtg tcggccctcg ccgctcgccc cgggaagcga cggatggtcc tcgtgacgac
     1321 gggctgggag aggatgccgg gccagaccct cgcccagctc ggcttcggcc gcgacacctg
     1381 gcgccagttc gacacctcga cctggaagga gacggaggcg atcctgcgcg ccgcgaaccg
     1441 ctccggcgtg acgatcgacg tcgtcgacgc caagggcttc gagagcgagc tcgacatcga
     1501 gctctccgag atgcgggaag tcggcaagtg gatcgcgcgc ctcaacacgc gggacgggat
     1561 ggcgaccatc gccgagacga ccggcggggc cttcgtggag aacgtgagct tctacgagcg
     1621 cgccctcggc gagatctggc gggaatcgag cgactactac tccgtcggcg tcaccctgcc
     1681 cgaggcgctt ccggccggaa aggtcctgaa ggtccacctc tcctgctccc ggcccggcgt
     1741 cacgctgaag acccgctcga gcgtcggcgc ccggagcgcc gcggaagagg cccgggacct
     1801 cgccgagtcc gccctccttg ccccgccgcc gccctccgcg ttcgcgatcc gcctcgagct
     1861 cggcgctccc cgccccgacg gcgtcttctc ccggcgtcgg gtcaccccgt ggcgcctctt
     1921 cgtctcgggc ggcgacctga cgttctcccc cgacggcgac cgcaggacgg ccgtcgtgga
     1981 ggtcgccttc gccgccatcg acgagtccgg gggacggtcc gaagtcgcgc ccgagaggtt
     2041 cgacgtgtcc gtcccggccg cgcggcttgc cgagacggcc ggccgcgacc tgcccctctc
     2101 cggcctcctc cgcacccgta ccgggcgcca ccgcatcgtc gccaccgtcc gtgacgtcgc
     2161 ctccggccgc accgccgtcg ccaccgcccc gctcgtcgac gactagcccc aggctcggtg
     2221 ccaggcgtga aatcgtgtat taacggagaa cgtcccgact gcagccgcga tcggcgatca
     2281 tccttccgcg atcgcggccg cgagcgtctc cgcgtcgagg aggtccttcg gccgaccggt
     2341 cgcccgcttg ttgcggacga gctctctccg ccccaggacg ccgacagtca ccccctcgat
     2401 cgggacctcg acccgtccgg cccaggcctc ctcaaaaccg actccggaga tgctcgtcag
     2461 gagatcgatc cggacgggca cgaccccgat ctggaagaca gtacccgggc tcgagaggtc
     2521 gtccgccgtc aggtcgaaca gcgaggcgcc gaactcacgg agcgccgcga ggacccgtcg
     2581 cgcgttctcg ggcgtcgcgc gaacccagat gtccaggtcg ccggtcgccc gcggaacgcc
     2641 gtgcgccgcg agggcatgcg cccccacgac gaggtattca gcccccgccg cgtcgagtgc
     2701 ggacaacatc tcgacgaagt cgcgctccat cgatccgttc tcccttgaag gcccaggcgt
     2761 tccgggtgag acgctcgacg agagcgatcc gctccgacgg cgtcatcgcc gccacgacgg
     2821 gatcgggcat cccgccctcc tccgcgaggg cgatccgctt cacgggccag gacgacctct
     2881 ccgcgcgacc gctcacgaag acgattctat tccccggtgg cgtgagcgcc catccgcacg
     2941 aaggccgcgg cttgcgccgc ggccttcgac cggaacatgg aaaaccgggg ctacatgacc
     3001 gccgtcggcg gggccggctc gtcggccgcg cccgccttgc cgagcggcac gtagatcagg
     3061 tacgccgcga ggagggcgac gaccgggatc gcgagccagg gggcgatccc cgcgaacgcc
     3121 tcgagctgcg ggaacttgtt gtcggagcgg aacgcgacga cgccggccac gaggaggccg
     3181 acgagcgcct cgcccgcgat caggccggac gccgcgagga cgcccgcgtt ctcgacgcgc
     3241 gccttctgcg ccacgttgaa gtcgcgcttg ccggcgagct tgtcgacgaa gccgcggatg
     3301 aggccgccga tgaagatcgc gaacgtcgtc tcgagcggca ggtacatgcc gaccgagaag
     3361 agcatcgggc tcttgaccct cacgaggatg agcgtgatcc ccatcgcgat cccgacgagg
     3421 acgagcggcc aggccatctc gccgccgacg atcccctgcg agagggccgc catgaggccg
     3481 gcttgcgggg ccgggaggtt cttgccgccg aagccgccga cgccagggtt cgacgccagg
     3541 tccgaggtgt ggaggaggta gagtgggaag aacatgacgg cgccggcaat gacgacgccg
     3601 atgacgtcgc cgacctgcat cttccacggg gtaccgccga ggatgtgccc gaccttcagg
     3661 tcctggagca tctcgcccgc gacggcggag gagacgcaga cgaccgcggc gaccgcgagg
     3721 acggccgcga ccccctcgac gcccttgacg ccgatgacga ccatcgtcag cgcggcgacg
     3781 atcgtcgtcg cgagcgtgag gcccgagatc gggttgttcg aggagccgat gaggccgacg
     3841 aggttcccgg agacggccgc gaagaagaag ccggtgatga gcatgacgat cgcggccagg
     3901 agcgctccgc cgaggacgtt cgtgaagtag aagtagaggg cgaccatcag gacgaagacg
     3961 gccgcgatcc cgaggaggac gaccttgaag gagaggtcct tttcggtccg ctcggtcgcc
     4021 tcggccgccg ccgccgactt cttcacgtcg gccacgccgc gcttgatgcc cgagatgagg
     4081 ttcttccgca tcttccagag cgtgaagcag gcgccgacga gcatgccgcc gaccgcgatc
     4141 ggccggacga tgtagcggta gaggtggccc gcgagcccgg cccagtcctg ggccccggag
     4201 gaggcgtccg tgtacctctc gatgaggttc gggccgagga agaagacgag gagcgggacg
     4261 aagaggcccc acgcgaggag gccgcccgcg aagttcaggg cgcccagctc gggtccgatg
     4321 atgtagccga cgcccatgta cgccggcgtg gcggccgggg ccgagatcgt cgtcacgccg
     4381 ccggcggcga tggagtcgga gtccttcgag aggcccagcc tcacgaagct ctccttcacc
     4441 ttgccgaccg cgacgtggaa gtcgttcgag gcgcggaaga cgcccaggtc gccgaggagc
     4501 ttgatgacgg cgccgacgcc catcgcccgg aagagctgga tcgccgcgtc ggccccgcgc
     4561 tgacccgcct tgtggatctc gctcgccgcg accgactcgg ggaacggcag ccccttgtcc
     4621 tcgaccatga cgcgccggag gatcgtcacg aagaggatcc cgaggaggcc gcccaggatc
     4681 atcagggccg tcgcgatccc gtactccttc gcgttgaacc cttcgaactt ccacatcccg
     4741 aggatgacga acgccgggat cgtgaagatc gcgcccgccg cgaccgactc gccgatcgag
     4801 ccgacggttc gggcgaagtt ctcctcgagg agggagccct tcatgatccg gaggacggcc
     4861 atgccgatga cggcggccgg gtacgtcgcg gcgatcgtca tcccggcctt caggccgagg
     4921 taggcgttcg cggcgcccag gacgacggcc atcaggaggc cgaggacgag cgcccggccc
     4981 gtgaactccg tcatggtgga gctcgccggg acgaacggct ggaacggttt ctcactcatc
     5041 ggggacgatc ctccctcgcg atctgatctc gatggatacg gctgtgaggc cggaggcgca
     5101 tcaaacgcgg attctacatg cgggcgcggc ctcgtccgcc cctcgaaggc cggtcctcct
     5161 caccgcccgg aggcggggct cccgtctcgc cgcggtgcgg cgcggccccg gaaacgggcc
     5221 gcgcggccgc ccgctccgct acgggacgac ggcgacgtgg aaatgcgcct ccagcgtctt
     5281 gtgctcgtag gtcgagggga gcggcgggaa cggggaggcc ttctcgacgg cggcgaggga
     5341 cgccttgtcc cacgctttca cgcccgactc catcgagacg aaggcgttct ggacccgccc
     5401 gtcgcggctg atcaccgcct ggacgacgca cttcttcccg accttcggga acgacgcggg
     5461 cgcctgccag gtggacgcga ccttctggaa gaccttcttc tggtaggccg ggtccttcag
     5521 cgtcgactgg aagtagacgt tcagggacgg ctgggccgcc agggcgggaa gcgcggcgag
     5581 gaggaggagg gaaaggagtc gcttcatctg cggtctgctc cgggtccgag gatacgactc
     5641 ccgcgggctc tctccgcgct aacctccgcg aatgcgcccg gtcctcgcct ccgcccgcac
     5701 ctcgctcctc cccgtactcg cgatcgcagc cgcgctcctc cccaccgcgc ccctcgcggc
     5761 ccaggagcgg cccgtcgtgc cgatccctcc cgatcactgg acgcagggcg agctcgacaa
     5821 ctttcgctcg accccgtcgt acgaccggac gctcgccttc ctccggcgcc tcgagcagac
     5881 ctcgccgtac ctctcgctct ccttctacgg cacctccgca gaggggcggc cgatgccggt
     5941 cgtcgtcgcc tcgaaggaga aggctttcac ccccgaggcg gccgcgaagt cgggcaagcc
     6001 ggtcgtcctc gtcctgaacg gcatccactc gggcgagatc gacggcaagg acgcctgcct
     6061 gatcctgctg cgcgacatgg cgctcgggaa ccgccccgac ctcctcgaca cgatgacgct
     6121 cctcgtcgtc ccgatctaca acgtcgacgg acacgagcgc gcctcgaagt gggcccgccc
     6181 gaaccaggac ggccccgtcg aggggatggg cttccggacg accgcccagg gcctcgacct
     6241 gaaccgcgac ttcctgaagg ccgacgcccc ggagacccgc gcgctcctct cgctcgtcgc
     6301 cgcgtgggac cccgacctct tcgtcgacga ccacgtcacc gacggctccg acgcccaggt
     6361 caccgtcacc gtctcgtacg gcggcgagcc ggcgaccccc gagccgctcc gctcatggct
     6421 cgagaccgtg atgcggccgg ccctcgcgga ggtcgaggag aaggggtgga agacctctcc
     6481 gtacatcgag tgggtcgacc cgctcgaccc gcacaagggg atcgaccccg ggccgaccga
     6541 gccgcgctac ggcacctgct acatgccgct ccggtcgatc ccctcggtcc tcgtcgagat
     6601 gcacgcgctc aagccctacg gcgagcgcgt gcgcgcgaac gccgcgttcc tctcggcgct
     6661 cctttcgcgg gtcggacgtg acccggcgcc gctgaagacg gcccgcgcgg cggcgaaggg
     6721 gacggcgaag ggggcccgcc ccggcacgcc gttcgtcctc gcgggcgaga ccgacctctc
     6781 ccgccccgag acgatcgact ttcccgctta cgcctgggag caggtcgtct cccccgtcac
     6841 cggccgcccg cgcctcgtct acgacccgaa gacgccgatg acggtcccga tcccctacta
     6901 ccgccacacg aaggccaccc tcaccgcccc gcgcccggcg gcctacctcg tcccggcggg
     6961 ctggcccggc atcgaggaga agctcgcggc tcacggcctc cgcttcacga agctcgccgc
     7021 gccgcgcacg ctccccgtcg gcacgtaccg cgcgagcgag gcgaagttcg ccgccgcctc
     7081 ctaccagggg cgcgtccggc tcaccgcgaa gatcgcccgc gccgtcgaga cgcgcaccgt
     7141 cccggccgga tcgctctacg tcccgctcga cgacccgctc gccccggtgg cgatgcacct
     7201 cctcgagccc gaagggcccg actccctctt cgcctggggg ctgatgagct ccgccctcga
     7261 gacgaaggag tacatcgacc cgcgcgtcct cgagccgctc gccgcgaaga tgctcgcgga
     7321 agacccgaag ctcgccgccg agtggaaggc gctcctctcc gacccgaagc tcgccgccga
     7381 cgcccgcgcg cgccaccgct tcttctacac gcggaccccc tactgggacg agtcggtcgg
     7441 cctcctcccc gtcttccggc tcgacgcgcc gctcgaggcg acggcgccgg cggccccggc
     7501 gccgtagaga cggcgcctct cgccgctccg cgcgagggag cgggcgctcc gaaccgggcg
     7561 cccgctcccc ggcccgccgc cgcctacttc agcgacttga tctgcgcttt cgggacgatt
     7621 tgccaggagg gccagccgtt cggcccgttc tgctggagca tcacgaggtg gtcgccctcc
     7681 ggcagcggct tctccaggcg gtagatctgg gtcgcggtga gccccccctt cccgaccagg
     7741 ctctgatcgg gcggaagcag gcgcgtccag atgatctcgg agaccgcgtg gaaggtgttg
     7801 tcgtagcaga cgtcgtagtt cacgccgccg tacgccaccc agtagtgacc ggcgaacttg
     7861 aagcacttca gcgccccgaa cgtcttcgtg ctcgtccgga cgttccccac ccacttcgag
     7921 tcgatgcaga cgcttcccgg catcgtcagg aactgccccg gctcctggcc gttcgtgatg
     7981 ccgttgatcc cgagcccctt ctcggccagc cacttgaagt tctggttgaa cgacccgcag
     8041 gcgaccgcct ggatctgccc cttcacgagg cctccgttcg cggccgcgac ccccgcgctc
     8101 tgagaccagg tccagtgagc ccgtccgtac tcgtagtagg ccttcatcac cttccggagc
     8161 gcctgcgcgg gagcgtcccc gaacgccgcc ccggagccgg gcgtgggagg cagcagccgg
     8221 gcgttctcga ggatcgccca accgggcatc acgttcgtca ccccggccgc gccgaggaga
     8281 ttctggatcg ccgcgtgcag tgccataccg cctccttcat ccctgctccg ggcccccgcc
     8341 gacgccgacg gcgcacggtc ggcccgagac ggccgatcgg catcggggga gctgcccgtg
     8401 ttctcgtggg gccagtatat cgagggtccg gtcgggcggg gcgctactcg tcgccctttc
     8461 gaatccactt cgggacctcc ggcgcgcgaa aggccgcgaa gcggtcgagc agctcctccg
     8521 ggtcctcggc gacgacgagc atcgcccggt gcgcctccct cacgaagcgc tcgcgcaccg
     8581 cgtggtcgag gaagccgacg agcccgtccc agtatcccgc ggcgctcagg aagccgcacg
     8641 gcttggcgtg gaggccgagc tgcccccacg tccacgcctc gaagatctcc tcgagcgtcc
     8701 cgatcccgcc cggcagcgcc acgaaggcgt cggagagctc cgccatccgc gtcttcctct
     8761 cgtgcatcga cgacgtcacg acgagctccg tcagcccgcc gtacgcgatc tccttccgca
     8821 cgagcgcctc cggcatcacc cccgtcaccc gcccgccgag gccgaggacc gtgtcggcca
     8881 cgatccccat gatcccgacg ctcgccccgc cgtagacgag cccgaggccg cgcgccacga
     8941 gcgcccgccc gagcgccctc gccagcgcgg cgtacgccgg gtcgcgcccc tcgctcgacc
     9001 cgcagtagac gcagacgttc ctcatcgctt cgccggccgc cggctcggcg cgggtcggct
     9061 cctccccggc ggcgcccgac gtcctccgcg cgccgccacc tcgccgagag ggcggtgctc
     9121 ggagcccttt ccgaacgtca cgccgacgta cgtgcccggc acccggacca cctggcggtg
     9181 ccacgccccg cgcgggacga cgaacgcgcg gcccgcgggc attcggacgg tcacctcgcc
     9241 ccccgggccc tcgagaacga ggtccatctc gccggtcacc atcaggagga gctcggcgcc
     9301 gttcggatgg cgctcccacg ccgcgccgtc gacgtcgagc gcgtagagcg cggccagcca
     9361 gcccgacccc gaaacgacgc gcccgacgtc cgggggaaac ggcggtcccg ccgcgagcgc
     9421 gctccagaac tccgcgccac ccggaaggac gacggcgcct ccctcgtcct cgaggaaggc
     9481 gtacgtgcgt gccgggtcga aggtcgaagg cctgcccccc atcacacgtg ctggtcgaag
     9541 aggatcgggt tcccgtcggg gtcgacgacg acgaagctcg ccggccccgc cgtgctctcg
     9601 tccgcctcct gggcgaacgt cacgccgcgg gccttcagcg ccttctgaat ctcgcgcacg
     9661 tcgacgaagg agtcgagctt ccgcgcgttc acgtcccatc ccgggttgaa cgtcaggatg
     9721 ttcctctcga acatcccctg gaagaggcca atcacgacgt cgccgttccg caggatcagc
     9781 cacttctgat cggcctgccc ggcgaagacc tcgaatccga gcttctcgta gaactccagg
     9841 gacgcgtcga ggtccttgac ggcgaggctc accgagaacg ttccgagctg catgggcttc
     9901 cctttctgcc gccggagcgg cgggtgcgcc gctccggcga gatcgtgccc ctcgcggggc
     9961 gaggccgagg tcatcgcgga tctcctcgat ccgagcggcc ctacggtgag ggcgtcagct
    10021 tgaaggagtc gagggactgc cggaacgacg gctcgagcga cgggtacgtc gacgccaggc
    10081 cggtgcaggt ggcgagaagg acccggtccg gcaggatcac cgccagggcc aggaagcgga
    10141 gccgcgggcc gctgatcgag ccctcgtagt cgagcaggac cgcggggcgg ccggagaccg
    10201 tccggttctc cttcgagtgg agcttcagcc cggcctcctg gaactgcgac tccgacaggc
    10261 gcacgaacgc ctcccgcgtc gtgcgcgtct cctggaccgt cacgttgacg ctcggggaga
    10321 acccgccgtc gggcggtccc gtgacgacga gccgaacgag cgaggtcccc tccgcgggag
    10381 ccccgaagcg cgggggcgcg agggagaagc cgaactgtgc gtcccggaac tccgacgcgg
    10441 cggcggacga cgcgaagagg ccgcaggcga gcgccacggc cggcatgagt cgggagcggg
    10501 cggacacgcg catgagggcc tccttcggtg agccggaatg gtaaggcccg cgaccggaat
    10561 tctctgccgg ttcgtgctcc tctcgaagac tcagcggccc gcggaagccc gcctcaccat
    10621 gatcacggtc gggactccgg cgggcgtcgc ctccgcgtcc tcccggtcct cgaagccgaa
    10681 cgacgcgtaa aggcggcggg ccgccgcgcc gtccgccgag gcgggcgcga aggtctggac
    10741 ggagaccggc ctcgccgggt cgagccgcgc gatcgccgcg gcgaggagct cgcggccgag
    10801 acccgccccg cgggcgcgcg tcgagacggc cagccattcg atcgcgccgc cctccgggga
    10861 gaagacgatc ccgccggcca gctcctccgc tccgtcctcc gacggcgcga ccgccgcgag
    10921 cgcgtggccg ccgtcgatcg ccgtctcgag cgccgcgcgg aactccggca cctccgacat
    10981 cgggccgaag aggtgctcca cctcccgcgc gaggcgcaac caccccggga cgtcccccgg
    11041 ctcggcgctc cggatgcgag cgggcctcgc gggcgccgcg cgcggagagg accttcgcga
    11101 gtcgtcgatc gcggagtcgc gcgtcgtcat ttcgcgcccg cggccgggag cacgcccagc
    11161 tcgcggagga gcgacgacac cagctgccgc cgcgcggcgc ccggccccca gcgctcccag
    11221 acctcctcgc cctcgaggtt cagcgcgaag taccaggtct cgtccgcccg ctccacgaac
    11281 ccgacgagcc acgcgagctc gcgggtcggc gtcacgtcgg ccgtccccgt cttgccgcgg
    11341 agcgtatagc ccggcccgcg ctccaggacc atgatcgacc gcaggacctc gaggctccgg
    11401 tccgagacgc cgaggcgccc ctcgttcatc cgctggagga acaggacctg ctcgtccgcg
    11461 gagatcctga ggccgccctc gagccagaac gcgtcgacgc ctcccgccag gctctcgttg
    11521 ccgtacccga agcgcttcag ggactccgcc atccgcttcg gcccgatgcg ccgcgcggtc
    11581 tcccggtagt accagtagac cgacctctgg aacgcggagc ggagcgtctg gtcccgggac
    11641 cagtcgtcag accagaaccc gtcccgcttc cggctctcgt cgaacgggat cacgaagtcg
    11701 gcatcggaga cgactccggt ctcgagggcg atcagcgtat gcggcacctt gaacgtcgag
    11761 gccggcagga gccgctcggc ggcgcgcgcc gggccgtgac ggacgacctc gcccgtccgg
    11821 ccgttccgga ggacgaacgc agcttcgacg ggcgcgccgc ccgcgaagaa gcggcgcagg
    11881 tccgcgacac gttccctcgc gaccgcgggc gcgcggacct cgcttccgcc cggggcggcc
    11941 accacgagcc ccgcgaggag cccgagcttc aggacggagg cattcatcgg cagcgtccct
    12001 tcagtagcgc tccggctccg gcacggtgag cgggccgccc gcgttcccgg tgttgaccgt
    12061 cgaggcgggc tcgaacagga ggacgtggac ctcctcttcc gcgaccgggc gatgctcgac
    12121 gccgcgaggg acgatcagca tctccccctc gcagaggcga acggagcggt cgcggaactc
    12181 catcgtgaac gagccgtcga ggacgaggaa cagctcgtcc tcggcctcgt ggctgtgcca
    12241 gacgaacggc cccttcagct tcgccagctt cacgtgctgg ccgttcagct cgccgacgat
    12301 cttcgggtcc cagtaggtgg agaaccggga gaggcgctct ttcaggttga cgacgttcat
    12361 ggcgggtgtc cgggagggta gcgcgtccgc gcggcctgcg ggtcaacgcc gaacgacgag
    12421 tcgtcgcccc gggcggatcc ggggacgttt ccgaggatgg cgtgccggca tcggaggacc
    12481 tcgtcgttcc gacctggtcc aaccacgacc ggggcgcggc gcggccacga gacgcccctc
    12541 tacggccggg cgaactcggg gatgcggaag cgtctgcggt aggcgcccgg cgcgaggccc
    12601 gtcgtccgcc ggaacaggcg ccggaagaac gccgggtcct cgtaccccac ctgccaggcg
    12661 agcttctcga tcggcgtgtc gctgcgctcg aggcggcgct tggcgctctc gatccggagg
    12721 cgttgcacgt aggcgatcgg cgccaggccg gtcgcctcgg tgaagcgccg cttgaaggtg
    12781 cgctcggcga gccgcgatct ctcgaccatc gcctcgaccg ggttggcgac cgagaagtgg
    12841 ccgtccagcc actcctgcgc gctccggatc tccgcgtcgc cgtggtcgcg ccgcccctcg
    12901 aagacgaggt acggcgtgag gccgtcctgg tgccactgca gcgcgaagag gcgcgcgacc
    12961 tcttgcgccg cggtcgctcc ggcgtatctc gcgatgaggt agaggaccag gtcgtgccag
    13021 gccatcgagg cccccgagct cacgagctcc tcgcgcctgc cggagacgac gagcacccgt
    13081 tccgggtgga ccgggacggc cgggaacgtg gccgtgaacg cgcgggcgta ggcgaagtgg
    13141 accgtggcgt cccggccgtc gaagaggccc gtctcggcga ggaggaagat ccccgagcag
    13201 gccgagcaga gcaccgcccc accctcgtgc atcgcccgga gccactcgac gaggcgggga
    13261 gatcgccccg tcgcccagcc tccgggcccg agcagcaccg acggcacgat cacgagatcg
    13321 gtcgcgtcga tctccgagac cgcccgctgc acgtcgatcg ggacgccgct cgccagcccg
    13381 aggcttcccc tcgcctcccc cacgatctcg acgcggaacg gaggccgcgc gccggcgccg
    13441 gcaccgagcg cgtccatgcg tgcgaacgcg ttcagcacgt cgaagaggcc gcccagcgtc
    13501 gagatcaccg cgtcgggcaa cgcgacgagg ctgacgcgaa gcgggtccgg cccggaccgc
    13561 tcggttcgtc ctctcatcgg cttcgacgtt acctcgcggc cctcgctccg cgccacggcc
    13621 cgcgggcccg cgcggcccgc cgggcgtggc acgaacggac cgttcccggg cgattccggc
    13681 cctggtggcg ctccgggcgc gacgccatgt tgtgcgggac gtccggagac ccggatacga
    13741 gaacgaggag gcgactcatg agctcacccg cgatcgacac cacccggctg gagtctctgc
    13801 tcgcgcgcgc cgtcggcgat ctgtccgccg gccacggcgg cgtaatggtc agcatcggaa
    13861 cgaagctcgg cctctacaag gcgctcgcgg gcgccggccc gctcagcccg cgggagctgg
    13921 cggagcgcac gggctgcgcg gagcgctacg tgagcgagtg gctgaacgcg caggtcgcgg
    13981 ggggctacgt ggactaccac gccgtcaccc gcacctacga gctctccccc gagcaggaga
    14041 tggtgctcgc cgacgaggag agcccggtct tcatgccgca cgcctggcag gtcaccgcga
    14101 cgatgtgggc cgacgaggag aagacgctcg aggccttccg caccgggaac ggcgtggcgt
    14161 ggggagacca cgacgagcgg ctctactgcg gcgtggcgtc gttctaccgg aacgcctacc
    14221 gcgggagcct cgtgtcgcag tggctgccgg cgctcgaggg ggtcgtggcg cgtctcgagg
    14281 ccggcatcgc ggtggccgac gtcggttgcg gccacggcca ctcgacgctc ctgatggccg
    14341 aggcgttccc cgagtcgcgc ttccgcgggt tcgacgtcca cccggcgtcc gtcgaagcgg
    14401 cccggcgcaa cgcggccgag gccggtctgg acgggcgcgt ctccttcgac gtcgccccgg
    14461 cctccggttt ccccggcggg agctacggcc tgatctgctt cttcgacgcg ctccacgaca
    14521 tgggcgaccc gctcggcgcg gcgcggcagg ccgcctccgc cctggccccc gacggcacgg
    14581 tgatgctcat cgagccgttc gcgaacgacc gcctcgagga caacctctcg cccgtggcgc
    14641 ggctctacta ctccgcctcg gcctcgctct gctgcgcgaa cgcgatcgcc gacggcggcc
    14701 ggctcgtcct gggcgcgcag gccggagagg cgcgcctcgc cgacgtcttc cgcaaggccg
    14761 ggttcacccg tttccgtcgc gcctctgaga cgccgttcaa cctgatcctg gaggcgcgcc
    14821 gctgatcccc gcgctcactt cccgccggcg ggggcgacct cgtactcgct ctggacgagg
    14881 ccgccggcga acgtgcgcgt cgcgacgtgc gtgaggagga cgtcggcgtc gagcgggccg
    14941 aagagcggga tgccctcgcc gatgaggacc gggacgcggg agaggacgag gcgctggatg
    15001 aggccggccc gcaggaacgc ctggatcgtc cggcctccgt ccacgtagac gtgcccgaac
    15061 ccgcgcgccg cgagctgaga gaagacctcg gccggttcgc cggccatccg ctcgacgacc
    15121 gctccggccc gccccggagg aaggggccga tggctcagga cgaagaccgg cttcgccccg
    15181 tagggccaat ccgggaacgg gaggacgacc tcgtaggtgt tccgccccat cacgagagcg
    15241 tcgaccgtgg cgaagaactc ctcgaagccg tgcggctcct cgcccgagcc tccggccgac
    15301 aggaagtcga acgtgccgcc gggccgcgcg atgaacccgt cgacgctcgt gcccacgaag
    15361 accgaagctc tcatgacgtc ctcctcgatc gggttagatc ggcgaggtcc ggcagcgcga
    15421 ggcgaggagg ccggtggccg cgccgaggtc gaggtccgcc acgagcaggc cttcccgtcc
    15481 gtacggctga tggcacagaa gagtaccgtc ggggcgcgcg atcgccgacg tcgtgccgga
    15541 accctcgctc gcgcagttca ccgacgcgaa gtagcacgtg ttctccgccg cgcggcacaa
    15601 gaccgccttc tcgtggaacg tgttggcggg gtcggcgaag gcgaccggcc gatagctccc
    15661 gggctcggcg acgtgggcgt gcggatggaa cacgacctgc gcgccgcggc gcacggccca
    15721 gcggacggtc tccggatacc gccacccttc gtggcagatc acgaccccga acgtcagctc
    15781 tccggcggtg aacactctcc gctcggtcgc gaacgccgga taggtgccct cctccgacgg
    15841 gtcgagctgt cccttgtcct gccaccccac gacccggccg tccggtccga tcacgcaggc
    15901 cgtgatctgc aggccgcggt cggtcacgcg ctccgtgccc aggatgaccg tgacgccggc
    15961 cgccccggcg gccttcgcca cgtcggccca cgcccgttcg aggaacgcgg ggtctggcgg
    16021 cgggagcgtc gtccccggcc accggtaccc gggaacgaag cactccggga agcagatcac
    16081 gagcgcgccc cgtcggcccg cctcggcgac cgccgccgtc gcctggagca cggactcttc
    16141 gggcgtcgcc ggaatgcgga cgttcgcgag cgcgatgcgg acggtgctca tgccgcgagg
    16201 cccgctccgc gtcgggcgcc tccgggcgct gcgcggggtc cccgccccga accgatgccg
    16261 ccggctctct ccatcacgtt ctccatcacg ttctccattc ccttcacctc gtcctacccg
    16321 gcgggggtcc ggtcgtcgac ctcgtcgagg aacggccggc cgaagcgccc gaggcggggt
    16381 ccgtgactcc acggcgagcg ccagctccag agcaccccga cggcccggcc gaggaggtcg
    16441 gcgacgggcc tcgttccgaa gtaccgtccg tcgcggctct cgtcgcggtt gtcgcccatc
    16501 acgagcacct cgccttcggg gacgcgcacg gggccccagg tccgggccgc gctcgccagc
    16561 gccggcaccg cccgtaccgg gtgcggcgcg ccgtgaagcc gctcggtccc caggagcacg
    16621 gtctgcccgg ccgcgtcctc gacgggcgga aggtagcgga cgggctgctc cttcccgttg
    16681 acgaggagga tgccgtcgcg cagctcgacg gtgtcgcccg ggaggccgac caggcgcttc
    16741 acgaacggca cgcggccgcc gtcgggattc cgcaggacga ccacgtcccc gcggtccggc
    16801 tccacggtgg agaagagccg cacgctcgtg aacggcacgc gcacctggta ggcggccttc
    16861 aggacgaaga cgcgatcccc ttcgaggatc gtcggcttca tcgaccccgt ggggacgacg
    16921 taccagtcgg cgaggacgga gcggccggtc aggatgagac cggcgacgag gagcagctgg
    16981 cggacgtccc ggaggagggc cttccgcttc gaggtcggcg tcatgtcctc cccggaacgc
    17041 acgccgcgtg cccggccagg acggcacgcg ccgtccgatg gggcgcgccg tcccgtcagc
    17101 tgctcctctc cgagcgggtc gccacgatcg gctcgccgtt cgtgacgatg atcgtgtgct
    17161 cgaactgcgc cgagcggctc tccgggtgcc cgacgagcgt ccacccgtct tccgcttccg
    17221 tcacgtgggc gctcctcgtc gagaggaacg gctcgatggc gatcacctgg ccgaggcgca
    17281 gccggcggcc gtcgccccgg tcgtagtagc tcacgatccc ttccggctcc tcgtgcagct
    17341 tccggcccac cccgtgtccg gcgaggttcc ggatcacccg gaacccgtgc gccctcgcga
    17401 cccgctccac ggcccgcccg atccggttga gcggggcgcc ggcgcgcgcc tcggagagcg
    17461 cgctgtcgag ggccacccgc gtcgcgtggc agagggagcc gttcgcgtcc gagaccggcg
    17521 ggacgacggt cgtccccccc gtgtcggcga agtagccctc cagctcggcc gagacgtcga
    17581 cgttcacgac gtcgccggcc cggatgaccc ggggaccggg gatcccgtgc gccgcctcct
    17641 cgttcacgct gatgcaggtc gagcccggga agccgtacgt cacccggggc gcggacctcg
    17701 cgccgagccg ctccagcagg cggccgccga tctcgtcgag ctcggcggtc gtcatcccgg
    17761 gctcgaccct ctcgagcatc tcgtcccgga ccctcgcgac gacccgtccg gcgttcagga
    17821 tcccctcaac gtcgcgctcg ttctcgaccg tcatctcgcc ctcccggcgt ctgcacgttc
    17881 ctcgcggggt cccgcgtccc gcggggagct cgatgcctgc cgcacgacgc gtcaggccgg
    17941 cggcggggcg agcggctcct ccgatcccgc gagccgggag cagtggagga gccagtaggt
    18001 gacgacggcg accgggccgc tcacgagcgc cgcgagcgtc cagagctcgg ggcccacgaa
    18061 gacgtgactt ccgccctcgg cgcgccgggt cgaggcatgg cgaaagaccg cgaccgcgaa
    18121 cgcaacgtgg acgaccgcgg cggccaccca gaacagcgcg aaggcgaatg gcgcgacgaa
    18181 agcgtacgcg tggatgaggc tactcgagat catgcggctc gcatcgtacg acgtggccgg
    18241 gacgggcgag gcgcgccggg gccggccacc tcttcgatca ggagcgaagg cgcttcgccg
    18301 agcaggcgag cgtggaccag cggacgaccc gggtcatggt gatgaagacg agggaccaga
    18361 gagcggcgcc gaggacggcg gcgagggcga ggtggccggg gcgagggccg aaggcggcgt
    18421 cgggaatgag gagatagacg aatccgagcc cgggcgccac caggaggacc gcgagggccg
    18481 ccaggaagac cgccccgagg accagaccgg cgacgtgagc gaccaggacg aggggggaga
    18541 cgacgacggc ggcgacgggt gaaggcacct cgcgcaggta ccatggcgtc cggcggagat
    18601 cggagccggg cgcgtcgccg aatgccgcag tgggagaagg tgtcatgacc gcctcctgcc
    18661 ttcggaccgg acggattccg ttgctcgcgg gacgttacta caggccggtg tcacccggac
    18721 cggggcggcc gcgacgccgc gccccggcac gccgcacccg ggtccgcttc aatcggcgaa
    18781 ccaggcctcc cgggtgatcc ggtactcgac ggcctcgtga ccgcgctcga accgggtccc
    18841 cgaaagggct ccgccgatct tctcgacggc cctgcgggag cggtggttct gcggtccgac
    18901 gaggaagagc acggtgtcga cgaagcggaa cgcgtggtcg agcatcagcc gcttcatctc
    18961 ggcgttgtgc cggccgcccc agtgcgagcg cgagaggaac gtccagccga tctccacctc
    19021 gcgccgctcc gggtcgtagc cgtggaagcg ggaggagccg acgatccgcc cgctgctccg
    19081 gtcgatcgcg acgagcgcgc cgcccgattc gagcgccccc gcgaagaagc gccggaagac
    19141 ctcctcctcg tggcgggtcg actccgggtg ctgctcccag atcagcgggt cggacgcgac
    19201 cgcgtagagg tcggcccagt cctcctcccg gagggggcgc agctcgagga gtattccgct
    19261 cagcgtcggc tgaaggtcga gcttcatctt cggatcgggc cccgtcagcc gcgccgggcg
    19321 gcttcgatgg ccgcgatgtc gatcttcctc atctgcatca tcgcctcgaa cgcgcggcgg
    19381 gcgacggcgg gatcggggtc cgcgatcgcg gcggtgagcg cgcgcggcgt gatctgccac
    19441 gagagccccc acctgtcctt gcaccagccg caggcgctct cctgaccgcc gttctcgacg
    19501 atcgcgttcc acaggcggtc ggtctcggcc tggtcgtccg tcgccacctg gaacgagaac
    19561 gcctcgctgg gccggaacgc cgggccgccg ttcaggccga ggcaggggat gccgatcacg
    19621 gtgaactcga ccgtcaggac gtcgccctcc ttgcccgagg gatagtcggc gggcgcgcgg
    19681 tagaccttcc cgaccgcgct gtccgggaac gtccgggcgt agaactgcgc ggcttccagg
    19741 gcggcgccgt cgtaccagag gcagatcgtg ttcttcggga ccatcgtcat tccccttcgt
    19801 ggtgtcgcgg gtcggtcgcg gacgggtcgg ccccgacccc ggcaccggtc tcgtccgatg
    19861 cgtccggtct cccggagaag tggctcctca cgagcgcctc gcaggcggcc cagagccgag
    19921 gggagccctt ctcgatcgtc ggcccgtagc gggcgagcac ctggtcccgc gtgagcccgc
    19981 tcgcgagcca ggtccctccg cccgtggcga cgttcgcgat cagcggctgg accctgtcga
    20041 gcgccttcgc gaacgcggca tcggcggtct cggcggcctc gaactcccgc cagagcgcga
    20101 ggagctcctc tccctccggc cgcgggagga tcccgaagat cctctcggcc gccgcctcct
    20161 cccgctcggc ctgctcccgc ggaggcacgc cggagtggag gggcgtgtcg ccggcgtcca
    20221 cctcgacgag gtcgtgaacg aggagcatcc ggatcacgcg ggacgcgtcg accggccccg
    20281 ccgcgaggtc gtgcaggacg agcgcgtaga gggcgagatg ccaggaatgc tccgcgctgt
    20341 tctcccggcg gcttcggtcg aggagcggcg actggcggac gacggacttc agcccgtcga
    20401 tctcccgcag gaacgcgagg cgcgaagcga gcgggtcttc ggccgccttc cgaagcgtcg
    20461 agaggacggc ccggagcctc gcggccagct cgggatcggc ggcgatgccc gcgtcgtcga
    20521 gcttgcggcc cgcgagagga acgacggtct cgtccacgac gcgcgcgccc atcacccgga
    20581 gcgtctcgac gagagccccg tgcgcgatcg tggcctgcgg ccgggcgttg agcaccgaga
    20641 ccggcttgcc gctcatctcg acgccgccga cgagccagtc gagggcgttc ttcaggacgc
    20701 cggagacgcc atgcgcgtac tccgggctgg agacgacgac cgcgtcggcg cgggcgacgt
    20761 ccgcgcgcca cgcctcaacg gacgggggaa ggcccgcctc ctccacgtcg gagctgaaga
    20821 acggcagcga gtcgagcggc accgagaagc cgacggcggt gtccgccggc gcgagcgccg
    20881 cgagggcgcg gaggagggac gtgttcgacg aagcctcgcg gaggcttccc gagacggcga
    20941 ggacccgcat gcgtccctcg agccccgggc cgccgcgctc agacacggag gagcccgcgg
    21001 aagcccctcg cggcgtagta cgactgcgcc ccgttgtggt acgcgaacac ccggccgtag
    21061 cggcggtcgc cgaagagggc cccgccgagc gcccggacgt cggccggcgt cgcgatccag
    21121 ctcgaggtct tcgtgtcgaa ctcgccgaag ccctggaggg tccggtactc ctcctccgtc
    21181 agcaggtcga tccccatcgc cgccgccatc tcgacggcgc tgctctccgg ccggtgctcc
    21241 ttgcgggcct ttcgcgcctc gccgtcgaag cagaggctcc ttcgccccgc cgggctctcc
    21301 gccgagcagt cgtagaacgt gatcgggccc gtctctccgc cgcgggcgag gacgtccggc
    21361 tcgcctcccg acgactccat cgcgcgcagc gaccggagcg ccggaaggtt gccctcgagc
    21421 cgggcgagga ccttcgccca cgcgaggccc ggatgacggt tcccgtgctt ctcgaagcgt
    21481 cccttcagga cgtgcaggag ctctgcgcgt tcgctcgtct tcatggtgtc tctcgtctcc
    21541 ttcgtgcgcc cgtccgtgcc gttgcgcgag ccgcggcccg gcgcagcgta cgagaggagc
    21601 tcctcggcct ccggagccgc gggccgcgat gcctcgctct cgcggccggc gccgcgaacg
    21661 cggccgatca gggcgccccg gtcgtcgcca tgcaggcgcc ggcggggtcg gtcacgacga
    21721 cggtgccgcc ggccggcacc tggaccgggc cgggaccggc gaagacgtcc tggccgcgga
    21781 cgaaggccag ggagtaggag ccggcaggca ccgagaaggt gctcaggccc gggacggcga
    21841 gcgtgccgag ctgtgtgccg ttcgcggaga ccgcgacgct cccgggcaga gcacagggct
    21901 gcgtgagctg aatctggagg ttgccgttgg cgaccggcga ggagggctcg gtgggcgtgt
    21961 cgctgcaggc cgtcgcgaga agccccgcgg cgatcagagc cgatgccagg atcgggcgag
    22021 cgttcatggg gacctccgtt tccgagaact gccgggaatc ataggacgac gcgcgaacta
    22081 tttccggacc tcgccgggcc cgaggccgag ccgcttgttc tcctcgagca gggcgatggc
    22141 ctccgcgacc cgcttgtttc gggtctcgtc gcgcttcgcg gacgagatcc atcccacata
    22201 gcgccgctgg tgcgaagggg gcagggcgtt gaagctcttc cacgcgagcg ggctcgtccg
    22261 cagcgcgccg gccagccact cggggacgcc ggacgtcggc tcccgggccg gtggcgtgtc
    22321 ggccggcgcc gagccccgcg ccgcgaggcc cgcgggagtc atcaggcccc gggccgccag
    22381 gtccgcggcc agcgccctgt tcgtctccga ccagcgggac gacttcgttc tcggcgtgaa
    22441 cctctgtgcg aaccgcaggt cgtcgatcct cttccggacc ccgtcgatcc agccgaagca
    22501 gagcgcctcc tcgagagcgt cctgatacgt gagcgccgcc cgccccgtgt gcttcttcag
    22561 gaacacgacc cagatggcct tgctctcggc gtgatggcgg cccagccact tccgccagtc
    22621 ctgtcggctc cggggctcga aggtctccgt gatgatcatg ctttcgcctg gcccagcttt
    22681 cggtcggccg ggctcagcgg cccggggagc gcggcgcgcc gggtgggatg tccgtggcgg
    22741 ccgtgacggc cgtggtcgcg ccgccgggaa ccggggccgg ccggccgacg cccgggcacg
    22801 gctccggcgc gtcggagagc aggacggccg tgaagacgag cgaggcccgc ttgtgccagg
    22861 tgcggtcgtt cagcggcgtg ttgtactccc ccgtcgtccg cttctctgcc gtctccatcg
    22921 agacgtctcg cgcgaagtag atggcgtgcg cccctttctg agccgccttc gcccgcaggc
    22981 ggctcacgat ctcctcgtcg gagtagtacc ccgagacgta cgcctccacc tctccgagga
    23041 ccgtgaacgg ctcctcggga ggccgggaga gcacgcgaac ggacgaaggc tccaccggcg
    23101 ggcaggcgac ggtgttgcgg tcggggctcg ggcggaagac cgtcgagacg cagccctgag
    23161 aaggcaggac gacggctgcc gcggacagga gcgcaatgct gaggcgactt ctcatcttct
    23221 tgtctcccgt cgaacgaaat acgcaagagt tgcacctgcg gcgggcgtcc tctccccggg
    23281 acgccgccgg cccgtgggca tccccggccg ggacgacccg tcaggtcacg tcgccggggt
    23341 acctcagcgt gaggcgcagc atgtcgaaga gctgtatccc gttctcgaag atcggctccg
    23401 ggtagctccg gacgaagaag tcgtggtcga tgctcgtcac ccgaaagccc tgccgctggt
    23461 agaacgcgag ctgatagccg aaggtgccgg tgcccacctc cagctgccgc gcgccggagc
    23521 cgcgaaagag ctcgacgacc catttcagca gcgccgttcc gtatccggac ttctggtgtt
    23581 ccgggagcac ggcgatgcac atgagctcgt gggcgcccgc gccgcgcggc tgcacgacac
    23641 acgcgcccac gacgaccgcg ccgcgaaatg ccacgaagca cctcgattcc ggaaggtagg
    23701 agcgaatctt cgcttccgaa ggatcggcga ggagcagcag ctccatcggg gcctctgcgg
    23761 cagggacctc ctgaatccgt agcgacatct cttcctttcc gccttcagga ctccggatcg
    23821 aggtcgtcgt cgagccgccc cgatgaccgg agggccggct ccctactcgg ttcccgagac
    23881 gttctgcccg atccagagcc ggttgccgtc cggatcgtcg atccggaact ccgtcatgcc
    23941 gtagaacgtg acctcgaggt ccggaatctc gagaccgttt cgtcggacgg cctcgtgata
    24001 ctcgaggatg tcgtccgggt acaggtagag gacgggatct cgaggagcgt ccggatggac
    24061 gttgatcgat tgatcgacca tgaggcgaca ttcgccgagg cggagcatcg cccaccccca
    24121 ctcctcgtgc ctcgtctcga ccgtgaagcc gagcttcccg tagaacgcga cgctcgcggg
    24181 cacgtgtcgg acgggcagca tcggtatgag gcggttcagc atgagtggct cctcaggaga
    24241 cgattctctg acgccggacc gcgggcgtcg atggcgagca cggccatgct gcgcgagccc
    24301 gggggacgcc gggtcaggca tgcctgtgac gacggaagac gtcaccgcag gccttgcacc
    24361 gataccgctt caggggcggc acgagcggaa gcccgacgat ggagagggcc gcggcctggc
    24421 gacggagggg gacgaagagc tcgagaccgg ccgatgcgcc cgaggggcag gaggagcgga
    24481 agtggggagt ggacgaggcg gagggctcgg gcaagtggac ctctgcaccg tcgtccgcga
    24541 ggacgtgcgc cgacggtccg aggatcgcct ccgcttgagc cacggaccct tcgggaacga
    24601 ggacctgaac gccacccact gcgatggctt cggtccagac ggtgcctgcg aagctctcgt
    24661 tctgcatgag cacggcgatg cccgccgctt cgagggtggc gcaggtcagc tgcgcctcga
    24721 agagattgaa gtcgtggcga atggtcacga agcgcggcgg ttcgttcatg agcgtgtcgg
    24781 aggaagagcc cgtcggagcg gcgccgtcat cgcggtccag ggtggtaacg tcgatcgcat
    24841 gtcaaccgta cccgtaatcg tcggcacagc gcttgccccg gtgatcggcg ttctggggcg
    24901 gttgtcgggc ctcgaccgtg accgcgccat gtacccggtc gcgctcatcg tgatcgccgc
    24961 gtattacgtc ctgttcgcga ccatgggcgg agaagaggcc ctgccggccg agctgatcgg
    25021 ggcgaccgtc tttgccgtga tcgcgatcat cgggttccgc accagcctct ggtgggtcgc
    25081 ggccgggatc gccggtcatg gggtcttcga cttcgtcgtg caccctcaac tgatcgtgaa
    25141 cccgggcatg ccggctttct ggccggcgtt ctgcgggtcg atcgaccttg cgctcggggc
    25201 gatcctcgcc gtcctgctgc tccgccgcgc catccctgcg cgcgacggag cgcccacgcc
    25261 gcgggcacac gagtgatcac gacccggctc gacggatcgt gatcacggac ttcccccgcg
    25321 agtgaccggc ggccagcgca cggaccgcgg ccggagcttc gctcagcgga tagcggcgat
    25381 cgacgaccgg ccggagcttt cccgtctcca gcatgtcctt caggacgcgc agcccctcct
    25441 ggttcacgaa cgcgagcggc gaccgaaacc tctgtcgggc ggtgaccccg atgacccggg
    25501 accaggcttg ccggcccatg ccgagccact tgccgccgcc ctcgccaccg aggatcacga
    25561 gcgtcccggt cggggcgagc gcgcgtcgca gcgcagccac gcggcggcgt cctgcgagat
    25621 cgatgatggc gtcgtacgtg cgggcgccgg tcgtgatgtc ctcgcgcgtg tagtcgattg
    25681 cgtcggccgc gccgagggag cgcacgaagt ccagcttcga ggtgctgcac acaccggtca
    25741 cctccgcgcc catcgcgcgc gcgatctgaa ccgcgcagga gccgactccg ccgcccgcac
    25801 cggtgatcaa gaccgtctgt cccgcctcga tctcggccgc gcgcaggccc acgagggcgg
    25861 tcgtcgcgga caccggcacg accgccgcct cctcgaagcc gactttcgac ggcttgggga
    25921 cgagcttcga cggcgaggcc cgcgtgtact cggcgaacga gccatcgccg atgccgaaga
    25981 ccgcgtcgcc cggcgcgaac gcggtcacct ccgcgcccac cctctcgacc accccggcga
    26041 gctcgaggcc gcgcaccggg ttgcgcggac ggcgcagccc ggcggcgagg cgcaccgcgt
    26101 agggcaaccc ctccatgagg tgccacaccc cgaagtcgat gcccgccgcg tggactcgca
    26161 cgacaacctc gtccggcttc ggtcgggggc gctcgatctc gcgaagctgg agcacgtcgg
    26221 ccgagccgta gcggtcttga acgatggcgg tcatgagcga gtcggtcgaa cgtgcggtca
    26281 cggggtgctc ctcatccgca caggcgcgcc gagagcggcg acggctgagg gcggcgccga
    26341 tcagcccgag ggcagggatc ccgcggggca cgagccggga ctcgtacatc agggcgtgtc
    26401 ccgttgaatg ttgaacttcg cctcggagag gcggctggtt gaaagg
//
LOCUS       DAOONL010000044        26138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1719_length_26138_cov_16.986121, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000044 DAOONL010000000
VERSION     DAOONL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 26138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26138
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1719_length_26138_cov_16.986121"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLP50_08525"
     CDS             complement(<1..550)
                     /locus_tag="PLP50_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51633.1"
                     /translation="MAEPLIKIENVVKQYTRDTQVLTVLDGLSLTVEKGDFVALMGPS
                     GSGKTTLLNLIAGIDRPTRGRILVGDAEVSAMGEGALAKWRSEHVGFIFQLYNLVPVL
                     TAFENVELPLLLTRLSKKDRRRQVETALGVVGLSDRMDHYPRQLSGGQEQRVAIARAI
                     ATDPTLLVADEPTGDLDAKSGEE"
     gene            complement(561..1772)
                     /locus_tag="PLP50_08530"
     CDS             complement(561..1772)
                     /locus_tag="PLP50_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA51634.1"
                     /translation="MSLTEPSNRTADLSALKIRRDERPDRGRWVLPAAATVIVVAVGA
                     FFLLGGRGVTLRSPEVSVTRVALVTPTQASTVLTASGYIVARSKAEISPKSVGRVAWL
                     NLEEGQRVRKGELVARLESQELEAQRKQYAAAREQALAELVNARTERQRAAQLLKEAV
                     GSQQAFDGADARVNALEAQVASIEAQVRYVEELIRNAEIYAPIDGVVTVKKAFVGETV
                     SPQGFGGSGSAGATFAVIVDLGSLEMEADINEQNLGRLSIGQPAEVALDAYPDRPYKA
                     RLRQIVPTADRQKGSVKVKIEILDRDARILPEMSCRVVFLNPEAKVDAEAKPKVMVPT
                     ASVVEVGAKKGVLLVRDGQALFRPLELGAAAGSQLEVVSGVDGGEEIVAEAAASDVKW
                     RAEQKVRVKKD"
     gene            complement(1858..3306)
                     /locus_tag="PLP50_08535"
     CDS             complement(1858..3306)
                     /locus_tag="PLP50_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPA51635.1"
                     /translation="MNGDLSPDTLRAWGGAAVERLARYLETIESRPVLARTKPGELVA
                     QFPPNAPEEPEPWEAIERDLDRLVEPNLTHWQHPGFMAYFATNGSVPGIVGETLAAGY
                     NQVGILWRTSPASNEMEQAMVRWLASFVGLPREFDGQLADTASAASLIALAAAREQAF
                     PDVRKTGLAGLPPAVVYGSELAHSSIDKACVVLGLGISGLRKIPTDAEHRMDVTALEA
                     AIAADRAAGRRPIAVVATAGTTAVASIDPLPEIAALCRREGLWMHVDAAYAGAAASLP
                     EKRPLFAGWEEADSIVWNPHKWLFLPLECTGLFFRRMERVRRAFSVVPTYLETPEGSA
                     GVREYMDYGIQLGRRFRALKAWILMRTFGARGLRERLRSHIDLALRLERDLRNEPDVE
                     VLAPVLFSVVVFRFAPAGLSEPERDALNQRVLEEVNREGRSFLSHGVVKGKYALHAAL
                     GSLRTEERHLDVLLDSLRSARALAETVKSPSP"
     gene            complement(3336..4409)
                     /locus_tag="PLP50_08540"
     CDS             complement(3336..4409)
                     /locus_tag="PLP50_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51636.1"
                     /translation="MPRPFGTDRVELAAPGHLRVTCSRPKDWVARRPAERGVVLHPGA
                     AVRWEEELWEVVAAGDTPEGGAWYELAPWDDQHAIRVLFPYDDSSEAIRSEDRREAER
                     RRVGRVVALLLSPVVGLLPGHVQERLEVQMEVRATTLTLASIPLPFGVGVVALLAVLM
                     SLVGAAYVAAGTGRAPAEAPIGSFLLFLVYLFPESLARFAIAMGQGRPMGSVLGLPLF
                     AIVRLAGLAPAPTPAGTAPESPAPDEERLRADRFLMLEPLLSFLSPADQRRLAAERGF
                     APVTWGKRTAWFLLVYPGLTAPAHAARLLIGNGGFWSFLLLALALGLGVEQVVRLRTL
                     SRGEPAPSVLGRLVAPFAAPLLR"
     gene            4458..6260
                     /locus_tag="PLP50_08545"
     CDS             4458..6260
                     /locus_tag="PLP50_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPA51637.1"
                     /translation="MPEGRLLRSLSKLHVRDLGRPVSGRSLRAQFDRAVAVVLRLLAL
                     LAIAALLLEHGFDLPAETKAAVRRLLEVVLLTFVGLGLLRLVTRDDRRAFLREHRLDA
                     VVWAVVLLGFASRQAGVAAPVHLFGLDPDDLAGAWLAATQGLIVLSILFGAVRGSRRL
                     VGRRVQPQLVILVSFVLLAAAGTGLLLLPRSTKAAGIDLVDAVFTASSAASITGLAVV
                     DTRTAFTPFGHAILLALFQAGGLGIMTLTTFFAFAFSGGTLRQYASLQSIVGEESLGR
                     IRTTLVEIAVATFAVEAAGAAVLYRHLPDSAVTEAGRLFSSVFHSVSAFCNAGFVLTP
                     TDGSAPGLAGNAPVQATIMVLVVLGGLGFPVLAMLGGILARRRRRPGLQTKLVLTVSA
                     LLLTFGTGALLLLEKGSPALGQSAADRFLAALFHSVSARSGGFNTVDLSTLAPASLFA
                     IVVLMWIGGSPASTAGGVKTTTVAVAFLTVFSIAAGKDRVELFRRRIGDASIQRAFAT
                     VVVSVILLSVALLGLLALERKPPLDLAFEAVSALSTVGLSTGITPALGAPAKLLLSVL
                     MIAGRVGLLGCVLAATPLRRDARHEYAREDVLIT"
     gene            6266..6970
                     /locus_tag="PLP50_08550"
     CDS             6266..6970
                     /locus_tag="PLP50_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPA51638.1"
                     /translation="MPRQFAVIGLGNFGSQLAAELTRRGAEVLAIDEREEAVDDLRDH
                     VTHTVVLDATDDQALAEQRVAEMDAVVVSFGSDFEAALLTVSVLRQIGVKRIIVRATT
                     VRHERILETLGVDEVVLPVNETVTRLAGSLMVEGLYDSFALSSDFAVGEVAIPEELLG
                     RKAGEVDFAADYGVSLVTIRRIVPETRILGLGTRMVERILGIPSPATPLERGDTLIVF
                     GPKKSLEKLAKQGEPG"
     gene            complement(6985..7335)
                     /gene="speD"
                     /locus_tag="PLP50_08555"
     CDS             complement(6985..7335)
                     /gene="speD"
                     /locus_tag="PLP50_08555"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPA51639.1"
                     /translation="MSPLGTHLLLDLAGAPFATLDDPAVVEAALVETVAAMGAHVLGV
                     HLHRLEPQGISGVVVISESHLTIHTWPERGEAAIDLFTCGDPERARAAVAALVARLGA
                     RVSTLREVSRGAAG"
     gene            complement(7340..8467)
                     /locus_tag="PLP50_08560"
     CDS             complement(7340..8467)
                     /locus_tag="PLP50_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51640.1"
                     /translation="MPSSPLPSPWLFSRRADVLLFGGPALVSLLFLAGGARLGLLDGD
                     LPVPLWLLLVVGVDVAHVWSTGWRVYADTEELRRRPALTLGLPAAAYAAGVVLFTAGD
                     LVFWRVLAYLATFHFVRQQYGWVALYRRKSGENAEEGAAWERRLDAAVVYGATLAPLV
                     WWHARLPRRFHWLIAGDYAAGLPAWAGPAALAAFETVFALWVGKEARRLATGRPVSWG
                     KVLVVVTTALVWHLGIVVFDSDYAFAVTNVLVHGVPYFGLVLQSVRSSAAARAAAGRK
                     PVLADLGARHAALFMAPLLLLAFLEEWGWDLLVWHENGALFPGGAIDPGPLLLSLLVP
                     LLALPQATHYLLDAWIWKVKPENRAAAQAVGLAAPADADAR"
     gene            complement(8480..9646)
                     /locus_tag="PLP50_08565"
     CDS             complement(8480..9646)
                     /locus_tag="PLP50_08565"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPA51641.1"
                     /translation="MSATVRPASVRRAGGCWPTPEQTLLLRAALLPEPEARAAYASWR
                     ETADIERVDPASYRILPLLAHNLGGADPVLAKARGVLRHTWSRNQRLFHQASSAVKAL
                     QATGIPVMLLKGAALGLRCYESLGHRPMNDVDVLVPPERSAEARTVLASLGWRPSYHV
                     VPGHLPYVHAVGLTIGPGLDLDLHWHAIYERRRPEDERPFWENAEPLRGRAIDALAPS
                     PTDLLMQVAAHGLRWSPSPPLQWAADLVVLLRAEGTRVDWDRLLARAREAGFVLHLDA
                     TLSFVHGELGASVPAGVLSDLRSARPTRRERLELAVRSSSPGLLPGLVAHWLDFRRVD
                     LEAAHGSRFRRFLVYLATIWGAPSLAALPRVALVKSTKRLLRARHASSGRGSGS"
     gene            complement(9643..10338)
                     /locus_tag="PLP50_08570"
     CDS             complement(9643..10338)
                     /locus_tag="PLP50_08570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPA51642.1"
                     /translation="MSGGARRISVLVPVFDAEKYLGEALDSALSQSFSPAEVVVVDDG
                     SQDGSLRVASSFGERVRVIRQAHAGIGAARNRLVAEATEEWLAFLDADDRWTPTALAT
                     HLKALEARPHADAALGRVVQFVSPELPEAARSLLACPEGPMPGAHPGASLLRKSAFSR
                     VGPFRVDLRVGEFVDWWMRGLEAGLDAASHDAVVLERRLHETNTGRREKAAVTDYLKV
                     VRQALARRRSGAP"
     gene            complement(10335..11018)
                     /locus_tag="PLP50_08575"
     CDS             complement(10335..11018)
                     /locus_tag="PLP50_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPA51643.1"
                     /translation="MTVVLPVWNGERFLGEALESIGRQSWRAFEIVVVDDGSTDASAE
                     IAGAYPGVHLVRQARAGVAAARNRGVAAAQGELIAFLDQDDRYTPDKLERQVGAFQAD
                     PALELCFAHQRLFLDGLSTPPSWVRPGWLDGPLPGLLPGTLVVRRRAFDAVGPFAEGE
                     PIASDSDWLMRARSRGLRELLLPDVLLERRIHAANQSANVAAGHLELLRAVRRAAKRG
                     APPAGGASA"
     gene            complement(11030..11998)
                     /locus_tag="PLP50_08580"
     CDS             complement(11030..11998)
                     /locus_tag="PLP50_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51644.1"
                     /translation="MTDAFRRAARAAGGEEERAFVIGGQQLVLRTAGPAMLPVLAPAL
                     AHLVAAPGEPAGLEVFAWDSASTGVELPAPASTEDGDRHVWQPGVGILTVLDDSRRRA
                     VVWVASAADVPSNEVAAPLRSFLHLAFYELGAQFVHAAAVGRADGGLLVVGRSGAGKS
                     TTALACLGSPLGVAGDDYVLATDGPEPRVHSLYSSAKLEPHQLRRFPHLSGAVVNPDA
                     AGEKPMILLCPRWAGSVSAGFPLRGAVTPRVVGRGPTRARRVGGAALLAALAPSTIFQ
                     LPGTGRHTLARIGSLLRHLPCWELELGENVSDIPLALERVLEEARP"
     gene            complement(12052..12495)
                     /locus_tag="PLP50_08585"
     CDS             complement(12052..12495)
                     /locus_tag="PLP50_08585"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPA51645.1"
                     /translation="MDSVLAVRSPQVISEILDGEAVIIDLETGTYYSLDPIASSIWDA
                     IAAGGSTATGIVEGIAAAYGKGVPEVEAALTPFFSRLAAERLVVTVSGSQAALGPFPK
                     ASANLTEPTLSRFDDIQDLLLLDPIHEVEEAGWPARKPDPGDEPA"
     gene            12678..13964
                     /locus_tag="PLP50_08590"
     CDS             12678..13964
                     /locus_tag="PLP50_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPA51646.1"
                     /translation="MSNHELSIVFFLALAAILLACRLVGYLARYVGQPQVVGEMIAGV
                     LLGPSLLGWIAPQAQAALFPKAAMPVLYVVAQIGLVLYMFLVGLDFDVELLRKRARSA
                     VTVSWAGILTPFALGTLIAWAIHGDTALFAEKVSVGEAALFMGAAMSITAFPMLARII
                     YERGLSGTSMGTLALAAGSADDAAAWCILAVVLASFSGHAAVAVWAIGGGLAFGLFAF
                     FVLRPLLARAGAWVERKQAYDSFALPGALMLLAAAAWYTDMVGIYAVFGAFVLGAAMP
                     RGEIAKRIEAQVQPLTVNLLLPLFFVYSGLNTRIGLVNSWALWGLALLVLLAATLGKG
                     GACYAAARLGGEPHREAVGIGALMNARGLMELIILNIGLERGVITPTLFSIMVLMAIA
                     TTLTALPVFNLAFGRKAVGEEAVARPATAPGPLLDD"
     gene            complement(13994..15649)
                     /locus_tag="PLP50_08595"
     CDS             complement(13994..15649)
                     /locus_tag="PLP50_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA51647.1"
                     /translation="MRPSRREAIAALAGLPALSSLLAACRRGPDEPAFSGAIVGADVL
                     RGHRIRTGELLSRPVARKESVGVAILGAGISGLSAAWTFRRAGFTDFRIYELEDEPGG
                     NARSGANAVSAYPWGAHYVPVPMYGNAALETLLEEVGAVAGRSADGAPEWAEEMLCAE
                     PEERLYFRGLWYEGLYPRAGASREDREELARFEREMHRFAGLRDAKGRRAFALPRRRS
                     SDDAEWTPLDRISMREWLTRGGYRGARLFWFAEYATRDDFGSSLDDTSAWAGIHYFAS
                     RLRGTAPNEPFEEPASFLTWPEGNGRLVKHLLKAAGGRIVTAAVVFDVVPRPGGATLR
                     WLDVPRNEVVEVAARHVVYALPRYTAAKVLAPWRESPPPFLRAFEYAPWLVANLTLSG
                     RPADRGFPLCWDNVLYDSRGLGYVVATHQGGRSHGPTVLTYYLVFAGEEPRKAREKLL
                     SATWKELAEAVLADLSRAHPDLPALVTNVDVYLWGHAMARPHPGFVWHPEREALERPV
                     GRVRFAHADASGLPLFEEAQDAGVRSAEAILREDGERFRTLLG"
     gene            complement(15651..17165)
                     /locus_tag="PLP50_08600"
     CDS             complement(15651..17165)
                     /locus_tag="PLP50_08600"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPA51648.1"
                     /translation="MTPSPVRPGPVYLTVLVIATCGLVYELVAGALASYLLGDTVTQF
                     SLVIGIYLTAMGAGAWLSKFIEKGVARRFVEIEIAVAFLGGFSALILSFAFLSPRWFH
                     PVFYGLVFAVGTLVGVEIPLMLRLLRRSVAFKDLVAQVLTFDYLGALAASLLFPLVFV
                     PTLGLFRTSLLFGLLNVAVAFTSIRLFRDEIGPAGGLLAKAGLVTVLLLGGFVAADRL
                     SGLAEEQQYADEVLLAKSSPYQRVVLTRNRAGFQLFLNGHLQFSSVDEYRYHEALVHP
                     AFAVVPGARRVAVLGGGDGLAVREVLRHPSVEEVVLVDLDPLVTRLARENPLFLELNG
                     GSLLSPKVSVVNDDAMKWLETETGLFDLVFVDFPDPNSFAVGKLYTKRFYSLLRRRLA
                     PDGAFAVQSTSPLFARRSFWIIDRTIAATGFETRPYHAAVPSFGEWGFVLASPRPFDV
                     PSDLLPGLRFLSRETLPALFSFGPDMAPWAEAPVNRLHDQVLVRTYESEWRKFE"
     gene            complement(17170..17391)
                     /locus_tag="PLP50_08605"
     CDS             complement(17170..17391)
                     /locus_tag="PLP50_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HPA51649.1"
                     /translation="MNWWQLHAGQVWSALVFSAIGIGVFGIFFLVLPRILPFSLKKEI
                     EEDQNVALGIVIAAVVLGMAFIIGSAIGS"
     gene            complement(17391..17561)
                     /locus_tag="PLP50_08610"
     CDS             complement(17391..17561)
                     /locus_tag="PLP50_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51650.1"
                     /translation="MLRRGFQVFGALLLTAYAWAGLAGWELPTNAKKGVVAAGARSAT
                     GFRAYSFWTGGK"
     gene            complement(17569..19551)
                     /locus_tag="PLP50_08615"
     CDS             complement(17569..19551)
                     /locus_tag="PLP50_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HPA51651.1"
                     /translation="MSSRAASCPSCGGEVLFRAGSSVVTVCPQCRSAVSRKGANLEAL
                     GVVAELVPTSSPFRLGMTAKPKAAGLKPFRIVGRLQLSTGEGTWDEWHVSFEDGRYGW
                     LAEAQGTFWVTRPLPAPTNPPPPDFPQIAPGQRLSFGGYGEFTVTDRRQALYTSAEGD
                     LPFAATPGAVFMYADLSGADGSVGTLDYGDDPGVDAFFVGRPVALAELGVEGLEGWSE
                     RKVAARASSLNCPACGAGLQLKDPANTVRVACTYCGSVLATPQDGATAAKFEVLARLQ
                     KLPFKPLLPLGGEGTLRGRPYAILGAVLKACTVDGVHYYWREYLLKETKSEAYHWLVE
                     SNGHWTLVEPAAAGEVSTAPRLAVYRGTRYKHFQSTTARVEAVLGEFYWEVKKGESTD
                     VSDYVAPPRILSEERYANEVTWSEGSYVPKEELESGFGLGAPLPTPEGVGSNQPWPHA
                     ETARGFLRTVALFAGIAVFLFVFFNIKADRKVVYQKRHDLSVSTTDPTIAPDAIAEAL
                     TVVTEPFEVPRSGNVEARIDAPTDNSWVYVGAVLLEEATGQAYGFGLQSDYYHGVDGG
                     ERWSEGSRSRTVYVGRVPAGRYVLRLEPELERGKAPPYYDIRLRSGVPRFAHLMAVLV
                     LLLLGPIALAISKARFEGRRWAESDYGGGSGSDDDE"
     gene            complement(19634..20998)
                     /locus_tag="PLP50_08620"
     CDS             complement(19634..20998)
                     /locus_tag="PLP50_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA51652.1"
                     /translation="MSERDLDDAWKLRFRDPLVEAAFREEQRTSSARILRPSVFVALA
                     TILAFGVLDPIAFPETWRSILVIRLLALALPVALLAFLATLPRFARWTRPLLEVGSVG
                     AGWAVAAMIAVGQPQEPGTTVYFAALSFPLASAAIGLLPSGFAASVVRAGLIVLAFPA
                     VALVRKLPGWGPPLPADVVVVSLGTLLLLWFFFSIAGYFREAWQRRTFVTMRLLETAS
                     EKVRSVAADLKRLNEEKSTFLGIAAHDLRNPLGVILGYAEMLRDEEFPRKEAADMAAK
                     IVVSAERVRDLVAGLLEVSAAEAGRVALERRPCDLFGIARRVVESQLEPAARKEIGIR
                     LDGSPVVVTGDPGAIQQVVENVVSNAVKFSPWRSTVVVSVHADGGAGLVDVEDEGPGV
                     SEADRARLFAKFARLSARPTGGESSTGLGLSIVKTLTEAMGGEVSLESPPGRGARFRV
                     RIPV"
     gene            complement(21144..22130)
                     /locus_tag="PLP50_08625"
     CDS             complement(21144..22130)
                     /locus_tag="PLP50_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPA51653.1"
                     /translation="MTYIVFIIVVALVFDYINGFHDAANSIATVVGTRVLSPRVAVVW
                     AAFFNFAAAFALETRVAQTIGKGIVRSDVVDPDLVLASLAGAIVWNLLTWWWGLPSSS
                     SHALIGGFAGAAFVKAGAGSLVMKGLLVTSAFIVLSPLLGLALGFLNFLGAQWAFRRW
                     RPDRVDRVFRKVQLASAAAFSFGHGMNDAQKTMGIIFALLVSTGHLSGSHIPFWIVLL
                     CFAAISLGTLSGGWRIVRTMSMRITPLKPIGGSCAELAGAATLVGASLGGIPVSTTHT
                     ITGAIMGVGASRSLSSVRWGVARSIVVAWVLTIPASALVAGLTWKLFDFLRG"
     gene            complement(22137..22757)
                     /locus_tag="PLP50_08630"
     CDS             complement(22137..22757)
                     /locus_tag="PLP50_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPA51654.1"
                     /translation="MRLFPREETFLPQFARGAAVILEAARVLDALAAAPVLDDERAVR
                     IKQLEHQGDQIAHETFDLLNRTWITPIDREDIHVLVRNLDDVLDFIDAAAARIVLYRI
                     ASTTPELKKITSIIVQSAEAIQKALSLLSDLKHSRQILDACVEINRLENEADTVSQLA
                     IGRLFNDSKDPIEVMKWKEIYDFVEGATDRCEDVANTLEAIVIKST"
     gene            complement(22828..23937)
                     /locus_tag="PLP50_08635"
     CDS             complement(22828..23937)
                     /locus_tag="PLP50_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51655.1"
                     /translation="MTTPEPPRPSSRAPAMARRAVLPLLLGLAGGLLASAVLGRILEG
                     AIAGRTEESAFSLLERVGPDVEAVLAAGGDPKDAVDRVGRQLALRATLVATDGRVLGD
                     SGVDRSRLDALESHAGRPEVRDALRTGRGVEARFSSTLGHRLVYAATRLRSGEVLRLA
                     FPEKDLAAWEAPYRRRVLLVSAAAGLLAGAIVVRLRFRHAAELDLVLRAVESVSQGRR
                     PANPGAVSEETAAVHSALGDLLDRESERNEAVLRQETVSRAIFEGIPTGLLVVDRELS
                     LLDANAAALGLLRLPPGALRRGEHVLELVREKAVTDLLREALETGRAAGPLRLATGSG
                     DLELRAEAIRVSGEAISGRPAAVAILREAASPRPS"
     gene            complement(23967..25520)
                     /locus_tag="PLP50_08640"
     CDS             complement(23967..25520)
                     /locus_tag="PLP50_08640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA51656.1"
                     /translation="MNLDTFRSQFFAGAPRYEWLEDLGRGGMGVVFKAKDRDLDEVVA
                     IKVLYGHIGDGDDDGAILARFKREISLNRKVKHPNVARMYDFGSGGGHPYITMEFVPG
                     QDLQSLILREGRIPPERAIRILRQICQGTQAAHEQGIVHRDLKSQNIIVGSGDVVSIL
                     DFGLARGAVDEKLTQEAVVLGTPHYISPEQAMGQSADVRSDLYSIGIIAFEMLSGVLP
                     FTADSALGIAMKQISEPVPGNLSLYPEIPPRLREAVHRALAKRREDRPQTAAALDAAF
                     ARAAGLPDAPGADDEEIALARAIDAALGDPAGGAWPQRPVDSLRDGDPTPVVPHRPRP
                     RPAPGAEPAPSPIRPSSGATTPPVTPTMRRAAGRPTVFVVMGDGADRISAGKAFVESG
                     CDAVEARSGEEILELLMSRVPDAVVMDIALPKADGFEVARILKATPTFTQVPVLLLGT
                     RVDRAQEHFARQVGASEVLSRPVSERELVERTWRLLATRGFYRDEAPSSRISRSTAPV
                     PPGSDSTPS"
     gene            25678..>26138
                     /locus_tag="PLP50_08645"
     CDS             25678..>26138
                     /locus_tag="PLP50_08645"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261142.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HPA51657.1"
                     /translation="MTSIDSINTALGAEAKSLLEHTCTGIPKETLHLPGPDVVDRIWS
                     MSDRPIPVLRSLQQLVDHGRLAGTGYLSILPVDQGIEHSGGASFAKNPSCFDPEGIVK
                     LAVEGGCNAVASTLGVLGAVARKYAHKIPFLLKLNHNEFLTYPNKFDQIMFA"
BASE COUNT         3822 a         9083 c         9734 g         3499 t
ORIGIN      
        1 tctcctcgcc cgacttcgcg tcgaggtcgc ccgtcggctc gtcggcgacg aggagggtcg
       61 ggtcggtcgc gatggcccgc gcgatggcga cgcgctgctc ctgtccgccc gagagctggc
      121 gcgggtagtg gtccattcgg tcggagaggc cgacgacgcc gagcgccgtc tcgacctggc
      181 ggcgccggtc cttcttcgag aggcgggtca ggaggagcgg cagctcgacg ttctcgaacg
      241 ctgtgaggac cgggacgagg ttgtagagct ggaagatgaa cccgacgtgc tcgctccgcc
      301 acttcgcgag cgctccctcg cccatcgcgg agacctcggc gtcgccgacg aggatgcggc
      361 cccgcgtcgg ccggtcgatt ccggcgatca ggttgaggag cgtcgtcttc cccgagcccg
      421 aggggcccat gagggcgacg aagtccccct tctcgaccgt gagggagagc ccgtcgagga
      481 cggtgaggac ctgcgtgtcg cgcgtgtact gcttgacgac gttctcgatc ttgatgaggg
      541 gttcggccat cgtttcctcg tcagtccttc ttcacgcgga ccttctgctc cgcccgccac
      601 ttcacgtccg acgccgccgc ttcggcgacg atctcctcgc ctccgtcgac tccggagacg
      661 acttccagct ggctccccgc cgccgcgccg agctcgaggg ggcggaagag cgcctgaccg
      721 tccctcacga ggaggacgcc cttcttcgcg ccgacctcga cgacggacgc ggtcgggacc
      781 atcaccttcg gcttcgcctc cgcgtcgacc ttcgcctcgg ggttcaggaa aaccacccgg
      841 cacgacatct cggggaggat cctcgcgtcc ctgtcgagga tctcgatctt gaccttcacc
      901 gagcccttct gccggtccgc cgtcgggacg atctggcgaa ggcgcgcctt gtagggcctg
      961 tcggggtacg cgtcgagcgc cacctcggcc ggctgcccga tcgagagccg cccgaggttc
     1021 tgctcgttga tgtcggcctc catctcgagc gagccgaggt cgacgatgac cgcgaacgtc
     1081 gcgccggccg aaccggagcc gccgaagccc tgcggggaga cggtctcgcc gacgaaggcc
     1141 ttcttcaccg tgacgacgcc gtcgatcggg gcgtagatct cggcgttccg gatcagctcc
     1201 tcgacgtagc ggacctgcgc ctcgatggag gcgacctgcg cctcgagcgc gttcacgcgg
     1261 gcgtccgcgc cgtcgaaggc ctgctggctg ccgaccgcct ccttcaggag ctgcgcggcg
     1321 cgctgccgct cggtccgggc gttcacgagc tcggcgagcg cctgctcgcg cgcggcggcg
     1381 tactgcttcc gctgcgcctc gagctcctgc gactcgaggc gcgccacgag ttcgcccttc
     1441 ctcacccgct gtccctcctc gaggttcagc caggccacgc gcccgaccga cttcggggag
     1501 atctcggcct tgctccgggc gacgatgtat ccggaggcgg tcaggacggt ggaggcctgc
     1561 gtcggggtga cgagggcgac ccgggtgacg gagacctcgg gggagcggag cgtcactccg
     1621 cggccaccga ggaggaagaa cgcgccgacg gcgacgacga tcaccgtcgc ggcggcgggg
     1681 aggacccagc gaccccggtc cgggcgttcg tcgcgccgaa tcttcagggc ggaaaggtcc
     1741 gcggtgcggt tgctcggttc tgtaaggctc atctcgctct ctcggagtca cttgcgggcc
     1801 acgggccgcc ctcggcggcc gtcggcgcag ctcgtccctc cggctctatc gtggaatcta
     1861 cgggctgggg ctcttcacag tttcggcgag ggcccgggcg gagcgcaagg agtcgagaag
     1921 aacgtcgagg tgacgctcct cggtccggag gcttccgagg gcggcgtgga gggcgtactt
     1981 ccccttcacg acgccgtgcg agaggaacga gcgcccctcg cggttcacct cctcgaggac
     2041 cctctggttc agggcgtcgc gctccggctc ggagagcccg gccggggcga agcggaagac
     2101 gacgacggag aagaggacag gggccaggac ctcgacgtcc ggctcgttcc gcaggtcgcg
     2161 ctcgaggcga agggcgaggt cgatgtggct ccggagcctc tcgcggagcc ctcttgcccc
     2221 gaaggtccgc atcaggatcc aagctttcag cgcccggaag cgtcggccga gctggatgcc
     2281 gtagtccatg tattcgcgga cccccgcgct tccctcgggc gtctcgaggt aggtcgggac
     2341 gacggagaag gcgcgccgca cccgctccat cctccggaag aagaggccgg tgcactcgag
     2401 cgggaggaag agccacttgt gggggttcca gacgatcgag tcggcctctt cccagccggc
     2461 gaagaggggc cgcttctcgg ggaggctcgc cgccgcgccg gcataggcgg cgtcgacgtg
     2521 catccagagc ccttcgcgac ggcagagggc ggcgatctcg ggcagggggt cgatcgacgc
     2581 gaccgcagtc gtcccggccg tcgcgacgac ggcgatcggc ctccgtcccg ccgcccggtc
     2641 ggccgcgatc gccgcttcga gagccgtgac gtccatccgg tgctcggcgt ccgtcgggat
     2701 ctttcggagg cccgagatcc cgaggccgag gacgacgcac gccttgtcga tcgaggagtg
     2761 ggcgagctcg gatccataga cgaccgcggg gggaaggccc gcgaggccgg tcttccgcac
     2821 gtcggggaag gcctgctcgc gcgcggcggc gagggcgatg agggaggcgg ccgaggcggt
     2881 gtcggcgagc tggccgtcga actcccgcgg gagcccgacg aagctcgcga gccagcggac
     2941 catcgcctgc tccatctcgt tcgacgcggg ggaggtgcgc cagaggatgc cgacctggtt
     3001 gtatccggcg gcgagcgtct cgccgacgat cccgggcacc gagccattgg tcgcgaagta
     3061 ggccatgaag ccggggtgct gccagtgcgt caggttcggc tcgacgaggc ggtcgaggtc
     3121 gcgctcgatc gcctcccacg gctcgggctc ctcgggggcg ttcgggggga actgcgcgac
     3181 gagctcgccc ggcttcgtcc gggcgaggac ggggcggctt tcgatcgtct cgaggtagcg
     3241 ggcgagccgc tccacggcgg cgccgcccca ggcgcgcagg gtgtcggggg agaggtctcc
     3301 gttcatggtc cgccgagtct attgcggcgc gaacctcagc ggaggagcgg agcggcgaag
     3361 ggagcgacga gccggccgag gacgctcggc gccggctcgc cccgggagag cgtccggaga
     3421 cggacgacct gctcgacgcc gaggccgagc gcgagggcga ggaggaggaa ggaccagaag
     3481 ccgccgttcc cgatcaggag gcgcgcggcg tgggccgggg cggtgaggcc gggatagacg
     3541 aggagaaacc aggccgtcct cttaccccag gtcaccggcg cgaagccccg ctcggcggcg
     3601 agccgtcgct ggtcggccgg cgagaggaac gacaggagcg gctcgagcat caggaaccgg
     3661 tcggcgcgga gccgctcctc gtccggggcc ggcgattccg gtgccgttcc tgcaggggtg
     3721 ggtgccgggg cgaggccggc gaggcggacg atggcgaaga gcggcagtcc gaggaccgac
     3781 cccatcggtc ggccctgccc catcgcgatg gcgaacctcg ccagcgactc cgggaagagg
     3841 tagacgagga agaggaggaa cgacccgatc ggcgcttccg ccggagcacg gcccgttccg
     3901 gccgcgacgt aggcggcgcc cacgagcgac atcagcaccg cgagaagggc gacgacgccg
     3961 acgccgaagg ggagggggat cgacgccagg gtgagcgtcg tggcgcggac ttccatctgg
     4021 acctccagcc gctcctggac gtgccccgga aggagcccga cgacgggtga gagaaggagc
     4081 gccacgaccc gcccgacgcg gcgccgctcg gcctcgcgac gatcctccga ccggatcgcc
     4141 tcgctcgagt cgtcgtacgg gaagagcaca cggatcgcgt gctggtcgtc ccagggcgca
     4201 agctcgtacc aggcgccacc ctccggcgtg tccccggcgg cgacgacctc ccagagctcc
     4261 tcctcccagc ggacggccgc tcccgggtgc aggacgaccc ctcgttcggc gggcctgcgc
     4321 gccacccagt ccttcggacg ggaacacgtg acgcgcaggt gcccgggtgc ggcgagctcg
     4381 acgcggtcgg ttccgaacgg gcgtggcacg accggcgact atacgttctg cgcggagcgg
     4441 aacgtacact cgcggtcatg cccgaggggc ggctcctccg atcgctctcg aagctccacg
     4501 tgcgggacct cgggcgcccg gtctccgggc gaagcctccg ggcgcagttc gacagggccg
     4561 tggcggtcgt cctgcgcctc ctcgccctcc tcgcgatcgc ggcgctcctc ctcgagcacg
     4621 ggttcgacct cccggcggag acgaaggccg ccgtccgccg tctcctcgaa gtcgtcctgc
     4681 tgacgttcgt cggcctcggc ctgctccggc tcgtcacgcg cgacgaccgg cgtgcgttcc
     4741 tgcgggagca ccggctcgac gctgtcgtct gggccgtcgt cctcctcggg ttcgcgagcc
     4801 ggcaggccgg ggtcgccgct cccgtccacc tcttcgggct cgaccccgac gatctcgcgg
     4861 gcgcctggct cgccgcgacg caggggctga tcgtcctttc gatcctcttc ggggccgttc
     4921 gaggcagccg gcgtctcgtg ggccgccgcg ttcagccgca gctcgtcatc ctcgtcagct
     4981 tcgtcctcct cgcggcggcg gggaccgggc tcctcctcct tccgcggtcg acgaaggccg
     5041 cgggcatcga cctggtcgac gccgtcttca ccgcgtcgag cgcggcctcc atcacgggcc
     5101 tcgccgtcgt cgacacccgg acggccttca cgccgttcgg gcacgcgatc ctcctcgccc
     5161 tcttccaggc cggcggcctc ggcatcatga cgctgaccac tttcttcgcc ttcgccttct
     5221 ccggcggcac gcttcggcag tacgcgagcc tccagtcgat cgtcggcgag gagagcctgg
     5281 gacgaatccg gacgacgctc gtcgagatcg ccgtggcgac gttcgccgtc gaggcggcgg
     5341 gcgccgcggt cctctaccgg cacctgcccg actcggccgt caccgaggcg ggacggctct
     5401 tctcgtccgt cttccacagc gtctcggctt tctgcaacgc cgggttcgtc ctcacgccga
     5461 ccgacggctc cgctcccggg ctcgccggga acgcacccgt ccaggcgacg atcatggtgc
     5521 tcgtcgtcct gggcgggctc gggttccccg ttctcgccat gctcggcggg atcctcgcgc
     5581 gtcggcgccg gcggcctggc ctccagacga agctcgtgct gacggtgagc gccctcctcc
     5641 tcaccttcgg gacgggcgcg cttctcctcc tcgagaaggg cagcccggcg ctcgggcaga
     5701 gcgccgccga tcgcttcctc gccgccctct tccattcggt gtcggcccgg tcgggcggtt
     5761 tcaatacggt cgacctctcg accctcgctc cggcctccct cttcgcgatc gtcgtcctga
     5821 tgtggatcgg cgggtctccc gcctcgacgg ccggcggcgt gaagacgacg accgtcgccg
     5881 tcgccttcct gaccgtcttc tcgatcgccg ccggaaagga ccgggtcgag ctcttccgcc
     5941 ggcgcatcgg cgacgcgtcg atccagcggg ccttcgcgac cgtcgtcgtc tcggtgatcc
     6001 tcctctcggt cgcccttctc ggcctcctcg ccctcgagag gaagccgccc ctcgacctgg
     6061 cgttcgaagc ggtctccgcg ctttccaccg tcggcctctc gaccgggatc acaccggccc
     6121 tcggcgcgcc cgcgaagctc ctcctctcgg tcctgatgat cgcagggcgc gtcggccttc
     6181 tcggctgcgt cctggccgcc accccgctcc ggcgcgacgc gcgacacgag tacgcccggg
     6241 aggacgtcct gataacgtaa ggaccatgcc gagacagttc gccgtcatcg ggctcgggaa
     6301 cttcggctcc cagctcgccg ccgagctcac ccgacggggg gccgaggtcc tcgcgatcga
     6361 cgagcgcgag gaggccgtcg acgacctgcg ggaccacgtc acgcacaccg tcgtcctcga
     6421 cgcgaccgac gaccaggcgc tcgccgagca gagagtcgcc gaaatggatg ccgtcgtcgt
     6481 cagcttcggg agcgacttcg aggcggcgct cctcacggtc tcggtcctca ggcagatcgg
     6541 tgtgaagagg atcatcgtcc gggccacgac ggtgaggcac gagcggatcc tggagacgct
     6601 cggcgtcgac gaggtcgtcc tgccggtcaa cgagacggtc acgcgcctcg ccgggagcct
     6661 gatggtggag gggctctacg actcgttcgc cctctcgtcc gactttgcgg tcggggaggt
     6721 cgcgatcccg gaggagctcc tcgggaggaa ggcgggagag gtcgacttcg cggccgacta
     6781 tggcgtctcg ctcgtcacga tccgccggat cgtcccggag acgcggatcc tcggcctcgg
     6841 cacgcggatg gtcgagcgga tcctcgggat cccctcgccc gcgacgccgc tcgagcgggg
     6901 cgacaccctg atcgtcttcg gtccgaagaa gtcgctcgag aagctcgcga agcagggcga
     6961 gccgggctga gccgccggga gccgtcagcc ggcggcgccc cgggagacct cccgaagcgt
     7021 cgagaccctc gcgccgaggc gtgcgacgag cgcggcgacg gccgcgcggg cccgctccgg
     7081 atcgccacag gtgaagaggt cgattgccgc ctcgccccgc tccggccagg tgtggatcgt
     7141 caggtgcgac tcggagatga cgacgacgcc ggagataccc tgcggctcga gccggtggag
     7201 gtggacaccg aggacgtgag cgcccatggc cgcgaccgtc tcgacgaggg ccgcctcgac
     7261 gacggcgggg tcgtcgagcg tcgcgaaagg cgccccggcc aggtcgagga ggaggtgtgt
     7321 cccgagcggg ctcacgcatt cacctcgcgt ccgcatccgc gggcgccgcg aggccgaccg
     7381 cctgagccgc cgcccggttc tccggcttca ccttccagat ccacgcgtcg agaagatagt
     7441 gcgtcgcctg ggggagggca aggagcggca cgaggagcga gaggaggagc gggccggggt
     7501 cgatcgcgcc tccggggaag agggcgccgt tctcgtgcca gacgaggagg tcccagcccc
     7561 actcctcgag gaaggcgagg aggaggagcg gggccatgaa gagcgcggcg tgacgcgcgc
     7621 cgaggtctgc gaggaccggc ttccggcccg ccgcggcccg cgcggcggcg gaagagcgaa
     7681 ccgactggag gacgaggccg aagtacggga cgccgtggac gaggacgttc gtgacggcga
     7741 aggcgtagtc ggagtcgaaa acgacgatcc cgaggtgcca gacgagggcc gtcgtgacga
     7801 cgacgaggac ctttccccat gaaacggggc ggccggtggc gaggcggcgg gcttccttcc
     7861 cgacccagag cgcgaagacg gtttcgaagg cggcgagcgc ggcggggccg gcccacgcgg
     7921 ggagcccggc cgcatagtct cctgcgatca gccagtggaa ccgcctggga agccgcgcgt
     7981 gccaccagac gagcggcgcg agcgtcgcgc cgtagacgac cgccgcgtcg aggcgacgct
     8041 cccacgcggc gccctcctcg gcgttctcgc ccgacttcct ccgatagagc gcgacccagc
     8101 cgtactgctg ccgcacgaag tggaacgtcg cgaggtaggc aaggacgcgc cagaagacga
     8161 ggtcccccgc cgtgaagagg acgacgccgg ccgcgtacgc ggccgccggg aggccgaggg
     8221 tgagcgcggg acggcgtcga agctcctcgg tgtcggcgta gacgcgccag ccggtcgacc
     8281 agacgtgcgc cacgtcgacg ccgacgacga ggaggagcca gagcgggacc gggaggtctc
     8341 catcgaggag gccgaggcgc gcgcccccgg cgaggaagag gagcgacacg agcgccggcc
     8401 cgccgaaaag gaggacgtcg gcgcggcggg agaagagcca gggggacgga aggggcgagg
     8461 acggcaccgg gcgccgagtc tatgacccgg acccgcgtcc ggacgacgcg tgtcgcgcgc
     8521 ggaggaggcg cttcgtcgac ttgaccaggg cgacgcgcgg cagggccgcg agcgacggcg
     8581 ccccccagat cgtcgcgaga tagacgagga acctccggaa gcggctcccg tgggcggcct
     8641 cgaggtcgac acggcgaaag tcgagccaat gggcgacgag tcctggcagg aggccgggcg
     8701 aagaggagcg gacggcgagc tcgagccgct cccggcgcgt cggccgagcc gaacggaggt
     8761 ccgacaggac gcccgccggg acgctcgcgc cgagctcgcc gtggacgaac gagagcgtcg
     8821 cgtcgaggtg gaggacgaag ccggcctccc gggcgcgggc gaggaggcgg tcccaatcga
     8881 cccgcgtgcc ctcggcccgc aggaggacga cgagatcggc cgcccactgc aggggcgggc
     8941 tggggctcca tctcaggccg tgcgcagcca cctgcatgag gaggtcggtg gggctcgggg
     9001 cgagggcgtc gatggcccgg ccgcggagcg gttccgcgtt ctcccagaac ggtcgttcgt
     9061 cttcgggccg acggcgttcg tagatggcgt gccagtgaag gtcgaggtcg aggcccggcc
     9121 cgatcgtgag cccgacggca tggacgtagg ggagatgccc gggcacgacg tggtaggagg
     9181 gacgccagcc gaggctggcc aggacggtcc gggcctcagc ggacctctcg ggagggacga
     9241 ggacgtcgac gtcgttcatc ggacgatgcc cgaggctctc gtagcagcgc agcccgagcg
     9301 cggccccctt caggagcatc acggggatgc ccgtcgcctg gagcgccttc acggcgctcg
     9361 acgcctggtg gaagagccgc tggttccggg accaggtatg gcgcaggacg ccccgcgcct
     9421 tcgcgaggac cgggtcggcg ccgccgaggt tgtgggcgag gagcgggagg atccggtacg
     9481 aggcgggatc gacccgctcg atgtcggcgg tctcccgcca cgaggcgtag gcggcgcggg
     9541 cctcgggctc ggggagaagc gccgcccgca ggaggagcgt ctgctcgggg gtcggccagc
     9601 acccgcccgc gcggcgaacg gacgcaggac gaaccgtcgc gctcacggcg cgccgctccg
     9661 gcgccgggcc agggcctggc gcacgacctt gaggtagtcc gtgaccgcgg ccttctcccg
     9721 tcgaccggtg ttggtctcgt gaagccgtcg ctccaggacg accgcgtcgt gcgacgccgc
     9781 gtcgaggccc gcttcgagcc cccgcatcca ccagtcgacg aactctccga cgcgaaggtc
     9841 gacgcggaag gggcccacgc gcgagaaggc gctcttccgg aggagggaag cgcccgggtg
     9901 agcgcccggc atcggcccct ccgggcacgc gagaagcgag cgggccgctt cgggcagctc
     9961 cggagagacg aactgaacga cgcggccgag cgcggcgtcg gcgtgcggcc gggcctcgag
    10021 tgctttgagg tgcgtggcga gggccgtcgg cgtccagcgg tcgtcggcgt cgaggaaggc
    10081 cagccactct tccgttgcct ccgcgacgag gcggttccgc gccgcgccga tgcccgcgtg
    10141 ggcctggcgg atgacgcgga ccctctcgcc gaaagaagag gcgacgcgca ggcttccgtc
    10201 ttgggagccg tcgtcgacga cgacgacctc cgcgggcgaa aaggactgcg acagggccga
    10261 atcgagcgcc tcgccgaggt acttctcggc gtcgaagacg gggacgagga ccgagatccg
    10321 ccgggccccg ccgctcacgc ggacgctccc ccggccggcg gcgcgcctct cttcgcggcc
    10381 cgccgcaccg cgcggagcag ctcgaggtgg cccgcggcga cgttcgcgga ctggttcgcc
    10441 gcgtggatgc ggcgctcgag gaggacgtcc gggaggagga gctcccgcag accgcggctc
    10501 ctcgcgcgca tcagccagtc ggagtcgctc gcgatcggct ctccctccgc gaagggaccg
    10561 accgcgtcga aggcccgcct ccggacgacg agcgtccccg gcagcagacc cggcaggggc
    10621 ccgtcgagcc agccgggccg tacccacgag ggcggcgtcg agagcccgtc gaggaacagc
    10681 cgctgatggg cgaagcagag ctcgagcgcg ggatcggcct ggaacgctcc gacctgcctc
    10741 tcgagcttgt cgggcgtgta ccgatcgtcc tggtcgagaa acgcgatcag ctcgccctgg
    10801 gcggcggcga ccccacggtt tcgggcggcc gcgacgcccg cgcgggcctg tctgacgagg
    10861 tgcacgcccg ggtacgcacc ggcgatctcc gcgctcgcgt ccgtcgagcc gtcgtccacg
    10921 acgacgatct cgaaggcgcg ccagctctga cggccgatgc tctcgagcgc ctcgccgagg
    10981 aagcgctcgc cgttccacac ggggaggacg acggtcacgg tgaggctgtt cagggtcggg
    11041 cctcttcgag gactcgctcg agcgcgagcg gaatgtcgga gacattctcg ccgagctcga
    11101 gctcccagca ggggaggtgc cggaggaggc tcccgatcct cgcgagcgtg tgccggccgg
    11161 tgccgggcag ctggaagatc gtgctcggcg cgagcgcggc gaggagcgcg gcgccgccca
    11221 cccggcgcgc ccgtgtcggc ccgcgaccga cgactcgcgg cgtcacggct ccccggaggg
    11281 gaaagccggc cgagacggac ccggcccagc ggggacagag aaggatcatc ggcttctccc
    11341 ccgcggcgtc ggggttcacg acggccccgg agaggtgggg gaagcgccga agctggtggg
    11401 gctcgagctt ggcggagctg tagagcgagt ggacccgagg ctccggcccg tcggtggcga
    11461 gcacgtagtc gtcccccgcg acgccgagag gggagccgag gcacgcgagc gcggtcgtcg
    11521 atttcccggc gccgctccga ccgaccacga gaagaccgcc gtcggcccgt ccgaccgccg
    11581 ccgcgtggac gaactgtgcc ccgagctcgt agaacgcgag gtgcaggaag gagcggagcg
    11641 gagcggcgac ctcgttggac gggacgtccg cggccgacgc gacccagacg acggcgcgcc
    11701 ggcgggagtc gtcgaggacc gtcaggatcc ccacgccggg ctgccagacg tgccggtctc
    11761 cgtcttcggt cgacgccggc gcgggcagct cgacgccggt cgaggcggaa tcccaggcga
    11821 agacctcgag gccggccggt tcccccggcg ccgcgacgag gtgcgcgagc gcgggcgcga
    11881 ggacggggag catcgcgggg ccggcggtcc gaaggacgag ctgctggccg ccgatgacga
    11941 acgcgcgctc ctcctcgcct cccgcggcgc gcgccgcgcg gcggaaggcg tccgtcatcc
    12001 ggtcgaacgt cgtttccgga agggaagcgg gcggcgtcgc cccgccatcc gtcatgcagg
    12061 ctcgtccccc gggtcgggct tccgcgccgg ccagccggcc tcctcgacct cgtggatcgg
    12121 gtcgagaagg aggaggtcct ggatgtcgtc gaagcgcgac agcgtcggct cggtgaggtt
    12181 cgccgaggcc ttcgggaagg gccctagcgc ggcctgggat ccggagacgg tcacgacgag
    12241 ccgctcggcg gcgaggcggg agaagaacgg cgtcagggcc gcttccacct cgggaacgcc
    12301 cttcccgtag gcggccgcga ttccctcgac gatgcccgtc gccgtcgagc cgcccgccgc
    12361 gatggcgtcc cagatgctcg aggcgatcgg gtcgaggctg tagtaggtgc ccgtctcgag
    12421 gtcgatgatg acggcctcac cgtccaggat ctcggagatg acctgggggg accggacagc
    12481 cagcacggaa tccatgacgt cgacagcctc cgcaacggga cattctcgga cggtttcggt
    12541 gcctttgcca cgcccgcctc atcccggaat cgccggcgga gtctagcggc acgcggagcc
    12601 acgggactcc ctcccggggc gccggccgac gatgtggtgt cgatcatcac cgggattccg
    12661 gttagagtgc gcgccgcgtg tccaaccacg agctgtcgat cgtcttcttc ctcgccctcg
    12721 cggcgatcct cctcgcctgc cgcctcgtcg gatacctcgc ccgctacgtg ggccagccgc
    12781 aggtcgtcgg cgagatgatc gccggggtcc tcctcggccc gtctctcctc ggctggatcg
    12841 ccccgcaggc ccaggccgcg ctcttcccga aggccgcgat gccggtcctc tacgtcgtcg
    12901 cgcagatcgg cctcgtcctc tacatgttcc tcgtcgggct cgacttcgac gtggagctcc
    12961 ttcggaagag ggcacggagc gccgtgaccg tctcgtgggc cggcatcctg acgccgttcg
    13021 cgctcgggac tctcattgcg tgggcgatcc acggcgacac ggcgctcttc gccgagaagg
    13081 tctccgtcgg cgaagccgcg ctcttcatgg gcgcggcgat gtcgatcacc gccttcccga
    13141 tgctcgcgcg gatcatctac gagcgcggcc tctcggggac ctcgatgggg acgctcgcgc
    13201 tcgcggccgg ctcggccgac gacgcggccg cctggtgcat cctcgccgtc gtcctcgcga
    13261 gcttctcagg ccacgccgcg gtcgccgtct gggcgatcgg cggcggcctc gcattcggcc
    13321 tcttcgcgtt cttcgtcctc aggccgctcc tggcccgcgc gggagcgtgg gtcgagagga
    13381 agcaggcgta cgacagcttc gcgctccccg gcgcgctcat gctcctcgcg gcggcggcct
    13441 ggtacaccga catggtcggg atctacgcgg tcttcggcgc cttcgtcctc ggggccgcga
    13501 tgccgcgcgg cgagatcgcg aagcggatcg aggcgcaggt ccagcccctc accgtgaacc
    13561 tcctcctgcc gctcttcttc gtctactcgg ggctcaacac gcggatcggc ctcgtgaact
    13621 cctgggcgct ctggggcctc gccctcctcg tcctcctcgc ggcgacgctc ggaaaggggg
    13681 gcgcctgcta cgcggccgcg cgcctcggcg gcgagccgca tcgcgaggcg gtcgggatcg
    13741 gggccctcat gaacgcccgc ggcctgatgg agctgatcat cctgaacatc ggcctggagc
    13801 gcggcgtcat cacgccgacg ctcttctcga tcatggtcct gatggcgatc gcgacgacgc
    13861 tcacggcgct cccggtcttc aacctcgcgt tcggaaggaa ggccgttggc gaggaggcgg
    13921 tcgcgcgccc ggcgacggca cccgggcctc tcctggacga ctgacggcga gctggcgtgc
    13981 cgcgagccgc ccgtcagccg aggagcgtcc ggaagcgctc tccgtcttcg cggaggatcg
    14041 cttcggcgga ccgcaccccc gcgtcctgag cctcctcgaa gagcgggagc ccggaggcgt
    14101 cggcgtgggc gaagcggacg cggccgacgg gccgctccag ggcttcccgc tcgggatgcc
    14161 agacgaagcc ggggtgcggg cgcgccatcg cgtgccccca gaggtagacg tcgacgttcg
    14221 tgacgagcgc agggaggtcg gggtgcgcgc gggagaggtc cgccaggacc gcctccgcga
    14281 gctccttcca ggtggcggag aggagcttct cccgcgcctt ccgcggctcc tcgcccgcga
    14341 agacgaggta gtaggtgagg acggtcggac cgtggctgcg ccccccctgg tgcgtcgcga
    14401 cgacgtagcc gagcccccgg gagtcgtaga ggacgttgtc ccagcagagg gggaagccgc
    14461 ggtcggccgg gcgtcccgag agggtgaggt tcgcgacgag ccacggcgcg tactcgaacg
    14521 cccggaggaa cgggggaggg ctctcgcgcc acggggcgag gaccttcgcg gcggtgtagc
    14581 gggggagcgc gtagacgacg tgccgggccg cgacctcgac gacctcgttg cggggcacgt
    14641 cgagccagcg gagcgtggcc ccgccgggcc gcgggacgac gtcgaagacg acggccgccg
    14701 tgacgatccg tcccccggcc gccttcaaga ggtgcttcac gaggcggccg ttcccctcgg
    14761 gccaggtcag gaacgaggcg ggctcctcga acggctcgtt cggggcggtc ccgcggaggc
    14821 gcgaggcgaa gtagtggatg ccggcccagg cggacgtgtc gtcgagcgag gagccgaaat
    14881 cgtcgcgcgt cgcgtactcg gcgaaccaga agagccgggc gccgcggtag ccgccccgcg
    14941 tcagccactc cctcatcgag atccggtcga gcggcgtcca ctccgcgtcg tcggaggagc
    15001 ggcggcgggg gagggcgaac gcgcggcgcc ccttcgcgtc gcggagtccc gcgaagcggt
    15061 gcatctcccg ctcgaagcgg gcgagctcct cgcggtcctc ccgggaggcg ccggcgcgcg
    15121 ggtagagccc ctcgtaccag aggccgcgga aatagaggcg ctcctccggc tccgcgcaga
    15181 gcatctcctc ggcccactcc ggggcgccgt ccgcgctacg gccggcgacg gcgccgacct
    15241 cctcgaggag cgtctcgagc gccgcgttgc cgtacatcgg caccgggacg tagtgcgcgc
    15301 cccacgggta cgcggagacg gcgttcgcgc ccgagcgggc gttcccgccc ggctcgtcct
    15361 cgagctcgta gatccggaag tccgtgaagc cggcgcggcg gaacgtccag gcggcggaga
    15421 ggccggagat ccccgcgccc aggatcgcga ccccgacgct ctccttccgg gcgacggggc
    15481 gcgaaaggag ctcgccggtc cggatccggt gaccgcgcag gacgtcggcc ccgacgatcg
    15541 cgccggagaa ggcaggttcg tccgggcccc gccggcaggc cgcgaggagg gacgagaggg
    15601 ccggcaggcc ggcgagcgcg gcgatcgcct cgcggcggga aggtctcacg tcactcgaac
    15661 ttccgccact cgctctcgta ggtccggacg aggacctggt cgtggaggcg gttgacgggc
    15721 gcctcggccc acggcgccat gtcgggcccg aacgagaaga gggcgggcag cgtctcacgc
    15781 gagaggaatc ggaggccggg gaggaggtcg gacgggacgt cgaaggggcg cggcgacgcg
    15841 aggacgaagc cccactcgcc gaaggacggg acggcggcgt ggtagggccg cgtctcgaag
    15901 ccggtcgcgg cgatcgtccg gtcgatgatc cagaacgagc gccgggcgaa gagcggggag
    15961 gtcgactgga cggcgaacgc gccgtccggc gcgaggcgac gcctcaggag agagtagaag
    16021 cgcttcgtgt agagcttgcc gaccgcgaag ctgttcgggt cggggaagtc gacgaagacg
    16081 aggtcgaaga ggccggtctc cgtctcgagc catttcatcg cgtcgtcgtt gacgaccgag
    16141 accttcggcg agaggagcga gccgccgttc agctcgagga agagcgggtt ctcgcgggcg
    16201 aggcgggtca cgagcgggtc gaggtcgacg aggacgacct cctcgacgga cgggtgccgg
    16261 agcacctcgc ggacggcgag gccgtcgccg ccgcccagga ccgcgacgcg gcgggcgccg
    16321 ggaacgacgg cgaaggccgg gtggacgagc gcctcgtggt agcggtactc gtcgacggac
    16381 gagaactgga ggtggccgtt caggaagagc tggaacccgg cccgattgcg ggtcaggacg
    16441 acgcgctggt agggggagct cttcgcgagg agcacctcgt cggcgtactg ctgctcctcg
    16501 gccaggccgg acagccggtc ggcggccacg aagccgccga ggaggagcac ggtcacgagg
    16561 cccgccttcg cgaggaggcc gccggcggga ccgatctcgt cgcggaagag ccggatcgac
    16621 gtgaaggcga cggcgacgtt caggaggccg aagaggagag acgtccggaa gaggccgagc
    16681 gtcgggacga agacgagcgg gaagaggagc gaggcggcga gggcgccgag gtagtcgaac
    16741 gtcaggacct gggcgacgag gtccttgaac gccacggatc gccgcagcag ccggagcatc
    16801 agcgggatct cgacgccgac gagcgtgccg acggcgaaga cgaggccgta gaagaccggg
    16861 tggaaccagc ggggagagag gaaggcgaac gagaggatga gcgccgagaa gccgccgagg
    16921 aacgcgacgg cgatctcgat ctccacgaac cggcgggcga cgcccttctc gatgaacttc
    16981 gagagccagg cgcccgcccc catcgccgtc aggtagatgc cgatgacgag cgagaactgg
    17041 gtgacggtgt cgccgaggag gtagctcgcg agcgcgccgg cgacgagctc gtagacgagc
    17101 ccgcacgtgg cgatgacgag gacggtcagg tagaccggcc ccggccggac cgggctcggc
    17161 gtcaccgggt cacgagccga tggcggaccc gatgatgaac gccatcccga ggacgaccgc
    17221 cgcgatcacg atcccgagcg cgacgttctg gtcctcctcg atctccttct tcagggagaa
    17281 cgggaggatg cgcggcagga cgaggaagaa gatcccgaag accccgatcc cgatcgccga
    17341 gaagacgagc gccgaccaga cctgtcccgc gtggagctgc caccagttca tcacttccct
    17401 cccgtccaga agctgtaggc ccggaatccg gtcgcgctcc gcgccccggc ggcgacgaca
    17461 cccttcttcg cgttcgtcgg aagctcccac ccggcgaggc ccgcccaggc gtaggccgtc
    17521 aggaggagcg caccgaagac ctggaaccct cgtctcagca tgtcggcctc actcgtcgtc
    17581 gtccgagccg ctccccccgc cgtagtcgct ctcggcccac cgacgtccct cgaaccgggc
    17641 cttcgagatc gcgagcgcga tcgggccgag caggaggagg acgaggacgg ccatcaggtg
    17701 agcgaaccgg gggacgccgc tccggagccg gatgtcgtag taggggggcg ccttcccgcg
    17761 ctcgagctcc ggctcgaggc ggaggacgta gcgcccggcg gggacgcgcc cgacgtagac
    17821 ggtccgcgag cgggagccct cgctccacct ctcgccgccg tcgacgccgt ggtagtagtc
    17881 ggactgcagg ccgaacccgt acgcctggcc ggtcgcctcc tcgaggagga ccgccccgac
    17941 gtagacccag gagttgtccg tcggcgcgtc gatccgcgcc tcgacgttcc cggagcgggg
    18001 cacctcgaac ggctccgtga cgacggtgag cgcctcggcg atcgcgtcgg gggcgatcgt
    18061 cgggtcggtg gtggagacgg agaggtcgtg ccgcttctgg tagacgacct tccggtcggc
    18121 cttgatgttg aagaagacga acaggaagac ggcgatcccg gcgaagaggg cgacggtgcg
    18181 caggaacccc cgggccgtct cggcgtgcgg ccacggctgg ttcgagccga ccccctcggg
    18241 cgtcggcagg ggcgctccga ggccgaagcc cgactcgagc tcctccttcg ggacatacga
    18301 cccttccgac caggtcacct cgttcgcgta ccgctcctcc gagaggattc ggggcggcgc
    18361 gacgtagtcg gagacgtcgg tcgactcgcc cttcttcacc tcccagtaga actcgccgag
    18421 gacggcctcg acgcgtgcgg tcgtcgactg gaagtgcttg tagcgcgtcc ctcggtagac
    18481 cgcgaggcgc ggcgcggtcg agacctctcc ggccgcggcc ggctcgacga gcgtccagtg
    18541 cccgttcgac tcgacgagcc agtggtaggc ctcggacttc gtctccttca ggaggtactc
    18601 gcgccagtag tagtggacgc cgtcgacggt gcaggccttc aggaccgcgc cgaggatcgc
    18661 gtagggtctt ccgcggagcg ttccttcccc gccgagcggg aggagcggct tgaacggcag
    18721 cttctggagc ctggcgagga cttcgaactt cgccgccgtc gccccgtcct gcggcgtcgc
    18781 gaggacggag ccgcagtagg tgcaggcgac gcggacggtg ttcgccgggt ccttcagctg
    18841 caggcccgcg ccgcaggcgg ggcagttcag cgaggaggcc cgcgcggcga ccttccgttc
    18901 gctccatccc tcgagcccct cgacgccgag ctccgcgagc gcgaccggcc ggccgacgaa
    18961 gaaggcgtcg acgccggggt cgtcgccgta gtcgagcgtc ccgacgctcc cgtcggcgcc
    19021 cgagaggtcg gcgtacatga agacggctcc cggggtcgcc gcgaacggca ggtcgccctc
    19081 cgccgaggtg tagagcgcct ggcggcggtc ggtcaccgtg aactcgccgt agccgccgaa
    19141 cgagaggcgc tggccgggag cgatctgggg aaagtcgggc gggggcgggt tcgtcggcgc
    19201 ggggagcggc cgggtgaccc agaacgtccc ctgcgcctcc gcgagccagc cgtaccggcc
    19261 gtcctcgaac gagacgtgcc actcgtccca cgtcccctcg cccgtcgaga gctggagccg
    19321 gccgacgatc cggaacggct tcagccccgc ggccttcggc ttcgccgtca tcccgaggcg
    19381 gaacggcgac gaggtcggga cgagctcggc gacgacgccc agggcctcga ggttcgcccc
    19441 cttccgggac accgcggaac ggcactgcgg gcagacggtg acgaccgacg acccggcgcg
    19501 gaacaggacc tctcctccgc aggacggaca gctggcggcg cgggagctca cgtgccggaa
    19561 ggatagaaca ggccgggcgc gacggggcga cccgacgccg tcgattcgtc gcctcgtcgc
    19621 ctggagaacg ctcctagacg gggatcctca cgcggaagcg cgcgccccga cccggcgggc
    19681 tctcgaggga gacctcgccg cccatcgcct ccgtgagcgt cttgacgatc gagaggccga
    19741 ggccggtcga cgactcgccg cccgtcgggc gcgccgagag gcgcgcgaac ttcgcgaaga
    19801 gacgggcccg atcggcctcg ctgaccccgg gcccctcgtc ctcgacgtcg acgaggccgg
    19861 ccccgccgtc ggcgtggacc gagacgacga cggtggagcg ccagggggag aacttgacgg
    19921 cgttcgacac gacgttctcg accacctgct ggatcgcccc cgggtcgccc gtcacgacga
    19981 ccggcgaccc gtcgaggcgg atcccgatct ccttccgcgc cgccggctcg agctgcgact
    20041 cgacgacccg ccgcgcgatc ccgaagaggt cgcacggccg cctctcgagc gcgacccgcc
    20101 ccgcctcggc ggcgctcacc tcgaggagcc cggcgacgag gtcgcggacc cgctccgcgg
    20161 agacgacgat cttggccgcc atgtcggcgg cctccttcct cgggaactcc tcgtcccgca
    20221 gcatctcggc gtagccgagg atgacgccga gagggtttcg caggtcgtgc gcggcgattc
    20281 cgaggaacgt gctcttctcc tcgttcaggc gcttcaggtc ggcggccacg ctgcgcacct
    20341 tctcgctcgc ggtctccagc agcctcatcg tgacgaaggt ccggcgctgc cacgcctccc
    20401 ggaagtagcc ggcgatcgag aagaagaacc agaggaggag cagcgtcccg agcgagacga
    20461 ccaccacgtc ggccgggagg ggcggccccc agccggggag cttgcggacg agggccacgg
    20521 cggggaaggc gaggacgatg aggccggccc tcacgacgct cgccgcgaag cccgagggga
    20581 ggaggccgat cgccgcggag gcgaggggga agctgagggc ggcgaagtag acggtcgtcc
    20641 cgggctcctg cggctgcccg acggcgatca tcgccgcgac ggcccacccg gcgccgacgc
    20701 tccccacctc caggagcggc cgcgtccagc gggcgaagcg cgggagcgtc gccaggaagg
    20761 cgaggagcgc gaccgggagg gccagcgcga ggaggcggat aacgaggatg ctccgccagg
    20821 tctcggggaa cgcgatcggg tcgaggaccc cgaacgcgag gatcgtcgcg agggcgacga
    20881 agacggacgg gcggaggatg cgggcgctcg acgtccgctg ctcctcgcgg aaggccgcct
    20941 cgacgagggg gtcgcggaac cggagcttcc aggcgtcgtc gaggtcgcgc tcactcaccg
    21001 ggacgacggg cccctcccgg cgtggcgggc gcggaatgcg ccatcccgag gaggagtgtc
    21061 gtcccccgag caccgttccg ctacccattg ctggggattc tgcaccggac cccgccgctt
    21121 cgcgtcgcac ttttcggtcg gggtcacccc cggaggaagt cgaagagctt ccaggtgagg
    21181 ccggcgacga gcgccgaagc ggggatcgtc aggacccagg cgacgacgat gctcctcgcg
    21241 acgccccacc ggaccgagga gaggctgcgg ctcgcgccga cgcccattat cgccccggtg
    21301 atcgtgtggg tcgtcgagac gggtattccc ccgagggagg cgccgaccag cgtcgcggcg
    21361 ccggcgagct cggcgcagga gcccccgatc ggcttcagcg gggtgatccg catcgacatc
    21421 gtccgcacga ttcgccagcc gccggagagc gtgccgaggc tgatggcggc gaagcagagg
    21481 aggacgatcc agaacgggat atggctcccg gagaggtgtc ccgtcgagac gaggagggcg
    21541 aagatgatcc ccatcgtctt ctgcgcgtcg ttcatcccgt gcccgaacga gaacgcggcc
    21601 gcggaggcga gctggacctt ccggaagacc cggtcgaccc ggtcggggcg ccagcgccgg
    21661 aaggcccact gggcgccgag gaagttcagg aagccgaggg cgaggccgag gaggggcgag
    21721 aggacgatga acgcgctcgt gacgaggagc cccttcatga cgaggctccc ggcacccgcc
    21781 ttgacgaagg ccgcccccgc gaagccgccg atgagcgcgt gggacgagga ggaggggagc
    21841 ccccaccacc aggtcaggag gttccagacg atcgcgcccg cgaggctcgc gaggacgagg
    21901 tcggggtcga cgacgtcgga gcggacgatc cccttgccga tcgtctgagc cacgcgcgtc
    21961 tcgagcgcga acgccgcggc gaagttgaag aaggccgccc agacgaccgc gaccctcggc
    22021 gagaggacgc gcgtcccgac gaccgtggcg atggagttcg ccgcgtcgtg gaagccgttg
    22081 atgtagtcga agacgagggc gacgacgatg atgaagacga tgtacgtcac ggcgtctcag
    22141 gtcgacttga tcacgatcgc ctcgagggtg ttcgcgacgt cctcacagcg gtcggtcgcc
    22201 ccctccacga aatcgtagat ctctttccat ttcatgacct cgatcgggtc cttcgagtcg
    22261 ttgaagagcc ggccgatggc gagctgggag acggtgtcgg cctcgttctc gaggcggttg
    22321 atctcgacgc aggcgtcgag gatctgccgg gagtgcttga ggtcggagag gaggctcagc
    22381 gccttctgta tggcctccgc cgactggacg atgatcgagg tgatcttctt cagctccggg
    22441 gtcgtcgagg cgatccggta gaggacgatc cgggcggcgg ccgcgtcgat gaagtcgagg
    22501 acgtcgtcga ggttccggac gaggacgtgg atgtcctccc ggtcgatcgg cgtgatccag
    22561 gtccggttca ggaggtcgaa cgtctcgtgg gcgatctggt ccccctggtg ctcgagctgc
    22621 ttgatccgca cggcgcgctc gtcgtcgagg accggggcgg cggcgagggc gtcgaggacc
    22681 cgcgccgcct cgaggatcac cgcggcgccg cgggcgaact gcggaaggaa ggtctcttct
    22741 cggggaaaca gtcgcatcgg gggctctcct ccgggcgaag gaggttatca ggggccgcgt
    22801 cgcggcgagg ccccgactcc cggcggctca gctcggccgg ggcgaggccg cttcgcggag
    22861 gatcgccacg gccgcggggc ggccggagat cgcctctcca gagacccgga tcgcctccgc
    22921 gcggagctcg aggtccccgg atccggtcgc gagccgaagg ggtccggccg cccggccggt
    22981 ctcgagggct tcgcggagga ggtcggtcac ggccttctcg cggaccagct ccagcacgtg
    23041 ctcgcctctc cgcagggcgc cgggcgggag gcgcaggagg ccgagggccg cggcgttcgc
    23101 gtcgaggagg gagagctcgc gatcgacgac gagaagtccg gtcgggatcc cctcgaagat
    23161 cgcgcgggag accgtctcct gccggaggac cgcctcgttc cgctcgctct cccgatccag
    23221 gaggtcgccg agcgccgagt ggaccgcggc cgtctcctcg gagacggcgc cggggttcgc
    23281 tggccgacgc ccctggctga cgctctccac ggcgcggagc acgaggtcga gctcggccgc
    23341 gtgccggaaa cggagccgga cgacgatcgc gccggcgagc agcccggccg cggcagagac
    23401 caggaggacc cgccggcggt agggggcctc ccaggccgcg aggtccttct cggggaaggc
    23461 caggcggagg acctcgccgc tccggagccg ggtcgccgcg tagacgagcc gatgcccgag
    23521 cgtcgaggag aagcgggctt cgaccccgcg tcccgtgcgg agagcgtcgc gcacctcggg
    23581 gcggcccgcg tggctctcga gggcgtcgag gcggctccgg tcgacgccgc tgtccccgag
    23641 gacccggccg tcggtcgcga cgagcgtggc ccggagggcc agctgcctgc cgacccggtc
    23701 gacggcgtcc ttcgggtctc ctcccgcggc gaggaccgcc tcgacgtccg gaccgacccg
    23761 ctcgagcagc gagaaggcgg attcctccgt ccgtcccgcg atcgcccctt cgaggatccg
    23821 tccgaggacg gcgctcgcca gcaggccgcc ggcgagcccg aggagcaggg gaagcacggc
    23881 cctccgagcc atggccgggg cgcgggaaga ggggcggggc ggctcgggcg tcgtcatgcg
    23941 agacggggga tcgtagcgcc cgcgcgttaa gacggcgtgg agtcgctccc gggcgggacc
    24001 ggcgccgtcg accgcgagat ccgtgaggag ggcgcctcgt cccggtagaa gccgcgcgtc
    24061 gccaggaggc gccacgtccg ctcgaccagc tccctctcgg aaacgggacg agaaaggacc
    24121 tcgctcgcgc cgacctggcg agcgaagtgc tcctgcgccc ggtccacccg tgtcccgagg
    24181 aggagcacgg ggacctgggt gaacgtcggc gtcgccttga ggatccgcgc cacctcgaat
    24241 ccgtcggcct tcgggagggc gatgtccatc acgaccgcgt ccggcacccg cgacatcagg
    24301 agctcgagga tctcctctcc gctccgcgcc tccacggcgt cgcagcccga ctcgacgaag
    24361 gcctttccgg ccgagatccg gtcggccccg tctcccatca cgacgaagac cgtgggtcgt
    24421 ccggcggcgc gcctcatcgt cggagtcacg ggtggcgtcg tggcgcccga ggaaggacgg
    24481 atgggggacg gggcgggctc ggcgccaggc gccggacgcg gccgcggacg gtggggcacg
    24541 acgggcgtgg gatccccgtc ccggagggag tcgacgggcc gctgcggcca cgcgccgccg
    24601 gccggatcgc ccagggccgc gtcgatcgcc cgcgcgagcg cgatctcctc gtcgtcggcg
    24661 ccgggggcgt cgggcaggcc tgccgcccgg gcgaacgccg cgtcgagcgc ggccgccgtc
    24721 tgcgggcggt cctcgcgtct cttcgcgagg gcccggtgga cggcctcgcg gagacgcggc
    24781 gggatctcgg ggtagagcga gaggtttccc ggcaccggct cggagatctg cttcatcgcg
    24841 atcccgagcg cggaatccgc ggtgaagggg aggacgccgg agagcatctc gaaggcgatg
    24901 atgccgatcg agtagaggtc gctcctcacg tcggcggact gtcccatcgc ctgctcgggc
    24961 gagatgtagt gcggcgtccc caggacgacc gcctcctggg tcagcttctc gtcgaccgcg
    25021 ccccgtgcga ggccgaagtc gaggatcgag acgacgtccc cggacccgac gatgatgttc
    25081 tggctcttca ggtcgcggtg gacgatcccc tgctcgtgag cggcctgcgt cccctggcag
    25141 atctggcgga ggatccggat cgccctttcc ggcggaatcc gtccctcccg caggatgagg
    25201 gactggaggt cctggccggg gacgaactcc atcgtgatgt aggggtggcc gcctccgctc
    25261 ccgaagtcgt acatgcgggc gacgttcgga tgcttgacct tgcggttcag gctgatctcg
    25321 cgcttgaagc gggcgaggat cgctccgtcg tcgtccccgt ccccgatgtg cccgtagagg
    25381 accttgatgg cgacgacctc gtcgaggtct cggtccttcg ccttgaagac gacccccatc
    25441 ccgccgcggc cgaggtcctc gagccactcg tagcgcggcg ctccggcgaa gaactgggag
    25501 cgaaaggtgt cgaggttcat gtcgcgtcgc ggcccgcggg cgagcgggtc gtcgcgcgcc
    25561 gaacctctat tatcgcccgg cgacgcgatt cccgcaggtg gcccgccgcc ctgtcgcgcc
    25621 ggcgtccggg gcgataggat gcctgagtcc cgcaacgaca cgcgaggaga cctgccaatg
    25681 accagcatcg actccatcaa cacagccctc ggcgccgagg cgaagtcgct tctcgagcac
    25741 acctgcaccg gaatcccgaa ggagacgctg catctcccgg gacccgacgt cgtcgacagg
    25801 atctggtcga tgtccgatcg tccgatcccc gtcctccgga gcctccagca gctcgtcgac
    25861 catggccgcc tggccggcac cggctacctc tcgatccttc ccgtcgacca ggggatcgag
    25921 cactccggcg gcgccagctt cgcgaagaac ccgtcctgct tcgacccgga agggatcgtg
    25981 aagctcgccg tcgagggggg ctgcaacgcc gtcgcgtcga cgctcggcgt cctcggcgcc
    26041 gtcgcgcgga agtacgccca caagatcccg ttcctcctga agctcaacca caacgagttc
    26101 ctgacctacc cgaacaagtt cgaccagatc atgttcgc
//
LOCUS       DAOONL010000045        25984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1742_length_25984_cov_19.917660, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000045 DAOONL010000000
VERSION     DAOONL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25984
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_25984_cov_19.917660"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLP50_08650"
     CDS             complement(<1..556)
                     /locus_tag="PLP50_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA51658.1"
                     /translation="MIRRGRPVLLLLESDPWRRESLGPFLVAEGYDVSTEDGEGRPEP
                     DLILFGLGDEPGSPTPRIERVRLRHPEAKVLVVVREVNARTTFPCLMAGVKGVLPHTA
                     GPAELRQALRCVREGTLWTPRAVLSEWVERVSRLGLGHVSAFTRSEQRVLEAVRDELP
                     NKEIARRLGVTEATVKFHVGKLLKK"
     gene            1105..1521
                     /locus_tag="PLP50_08655"
     CDS             1105..1521
                     /locus_tag="PLP50_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPA51659.1"
                     /translation="MFAHHAARTFALTLGLVALLASASSAPAAEESTAAAKKSVVNLN
                     QASADELARLPRVGPSLAGKIVAHREQHGPFKRAQDLMEVKGIGEKMFALLEPYLAVS
                     GPTTLTEKVSSKGTTARKAAKPAKAKAPAVAEKAGR"
     gene            1597..2202
                     /locus_tag="PLP50_08660"
     CDS             1597..2202
                     /locus_tag="PLP50_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51660.1"
                     /translation="MPPARRSLRGITLPEILTTLAILSLAAGLSAAAAHGLAGQTSLR
                     AAAHEVASVFTQARGRAIHRGIQCGVKWIARDGDLTLQIHDDGDGDGVRSDDIEGGVD
                     PLVYGPVSVKKRWPKVTVGFIPGFVARDPKGNPVGDLSDPVRFGRSDIASFSPLGDCS
                     PGSVWLGDARSRQSLVRLSPTTATIAIYEWAAARQSWIRIW"
     gene            complement(2227..2955)
                     /locus_tag="PLP50_08665"
     CDS             complement(2227..2955)
                     /locus_tag="PLP50_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPA51661.1"
                     /translation="MKALVPAAGFGTRFRPATLTTPKPLLPLLGVPILVRLFRHLKAQ
                     GVTSAVLNAHHLAETLMAAVGDSCEGIPVAFSTEDPILGTAGAIRRASERGLLGDGRF
                     LVVNGDLFTTLPFAPLLAALDAHGVVSALGVLPHAAPGETALWGDADGRLVSLGDVRP
                     FPEATGPWLFTGLQCAAPALVPRIPPGVSELARDVLRPSTERRDGAFALVPYRVPEDG
                     LWFDLGSPAKLAAAEEAIAASGLA"
     gene            complement(2952..3518)
                     /locus_tag="PLP50_08670"
     CDS             complement(2952..3518)
                     /locus_tag="PLP50_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPA51662.1"
                     /translation="MSDAPRETEAKFRVADRPALEERLAALGAVPGARQDEVNLLLDD
                     DDGSLRSRGCALRVRTVGGRGLLTYKGPASFDGGVKGRLELESGVDAPGNVLALLEAL
                     GFRPRFRYDKRRTPWRFAGPARPLVVVDETPLGLFAEIEGESGTVRALAAELGVAEES
                     FLRASYFGLWQAAREVDPDLPEDMVFGE"
     gene            3537..5183
                     /locus_tag="PLP50_08675"
     CDS             3537..5183
                     /locus_tag="PLP50_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA51663.1"
                     /translation="MPRYRLSGLRLRAPQAPDGTLLARLLGVPPGDVSGVEVVRRSLD
                     ARKKPDLVYEYAVELTLASEPAAPAAPPLALEPAPEPRAPELLLPPLERKARVAVVGT
                     GPAGLFAALALADAGVSVVVLERGDAAEERYRRVMRFWKSGDFDPESNVQFGEGGAGT
                     FSDGKLTCGKRHDKIAVVLETLHRFGAPDTILYDGKPHIGTDRLVVVLRNIRKHLRER
                     GAELRYRAKVVDVLREGEDGLLRALLLESGEEVPCDAAIFALGHSARDTVERLLARGL
                     AMSPKAFAMGVRIEHPQDDVDRIQYGRLAASCGVMPADYKMTAQTSVGRAVYTFCMCP
                     GGEVIACSSEPEGVVTNGMSRYKRASGFANSGLVVQTHLGDFAGESGPEVLRGIAFQR
                     RVERTAFAAAGGTYAAPAIRVTDFLSGRAPRPLPRGTYTPSCVPARLEGIYPEAYLTA
                     LAEALRSFERKMRGFVSDEAVLIAPESRTSSPVRIERNEACESPSLPGFFPAGEGAGF
                     AGGIVSAALDGLRVARAVRARLAGEVPPPSSARPVEAPEY"
     gene            complement(5188..5982)
                     /locus_tag="PLP50_08680"
     CDS             complement(5188..5982)
                     /locus_tag="PLP50_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51664.1"
                     /translation="MKATTTRPATPEERLRLEAYLHPKPKSNPAWAATVMVTVPLAWA
                     LFMILNMLLGGLVPAAVRLLLAVAIAGGGAWHFGKRLSAQHAESARPAEPVLGFLQRD
                     LESGRVVVNRWEADAFVKVASDPGRFVSTTWFAKLTDGTVVLLAQPDLEEAELSGDFP
                     ATSFEIATGEESRHVLSIRRTGERLEPVALRAPLSDAEWEELGDEADAPVPFGWEEVL
                     ARAAANPLRARGKGEARSRQEALEREILKAHEEEAGRATPDTTRPT"
     gene            6063..7970
                     /gene="selB"
                     /locus_tag="PLP50_08685"
     CDS             6063..7970
                     /gene="selB"
                     /locus_tag="PLP50_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPA51665.1"
                     /translation="MARVLVGTAGHIDHGKSALVRALTGTDPDRLPEEKARGITIDLG
                     FAHAAWGGTTFSFVDVPGHEKLVRTMVAGAAGIDVALLVVASDDSVMPQTREHLAILS
                     LLDVRCGVVARTKADLVDEETGAVVDAEITDLVSGTFLEGAPVVPVSALTGRGLDALR
                     DALFREASAADLRDPSARPARLFIDRVFTMKGFGPVVTGTLDSGRVRVEDRLTLLPEG
                     RELRVRRLEVHGEEREAAEAGERTSLNLAGIETSELGRGMALVAPGSLAVSTLLTVEV
                     TALPDLDDPLSDGLSVRLHIGTADVAARLHLLDGGAGAPSLPPGERVVAQVSALEPVA
                     ARRGDRFVLRRPSPQETVGGGRVLDVGRPRVRRRAPLSAHAAAVLAGDDERAVAVLLL
                     SEAGPAGLEIAELANRLGVPDAAAAAHVCALVESGAALRLTPALAVAASASVEMATRA
                     DAFFAERRKAGVPTLLVGRRELLGKIARGLPETTAEAWLATLAAAKRLVVTGDQAGPP
                     GAAASGVADEAGAFASRIEEAWRTAGFDAPGNLDLAKALGTKPQVVAGLVQHLLKSGA
                     LVRLSPEVFVHAEPLAGIEAKVAEQKGRTMSVGDFRDLLGLSRKYLIPLLEHLDRKKV
                     TRRVGDARLVV"
     gene            8041..8325
                     /locus_tag="PLP50_08690"
     CDS             8041..8325
                     /locus_tag="PLP50_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HPA51666.1"
                     /translation="MATTTVKATYSLDPGTVLRLEEIARSWGVSKSEALRRAIRAAAS
                     GEAPASGGPLDALDALQQAAALTPASARAWAADARSERRAPRAQAGKCSR"
     gene            8322..8735
                     /locus_tag="PLP50_08695"
     CDS             8322..8735
                     /locus_tag="PLP50_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPA51667.1"
                     /translation="MTHLDTSFLIRALVPGSPEDATLRAWLRRGLPVAASAVVWTELL
                     CGPLTAREAELALLVVGEPVPFCGEHAALAASLFNATGRRRGSLADCMVAAAAIRSEA
                     ELATANEADFARFVPLGLRLAPAESARPARPRPRK"
     gene            complement(8773..9558)
                     /locus_tag="PLP50_08700"
     CDS             complement(8773..9558)
                     /locus_tag="PLP50_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPA51668.1"
                     /translation="MIRALLERISQKHRTMAWPDGPPPALPSRYRGLPAIDASRCPEG
                     CRACADVCPTEAIAIAGGKPSLDLGRCLFCPECEAACPEKAISFTSDYRLAVRTREDL
                     TLDGAALPLARALEGRLLSLFGRSLKLRVVSAGGCNACEADVNVLGTIGWDLGRFGIQ
                     VVASPRHADGVLITGPVTKNMELALRKTWDAVPPPKIVIAVGACAISGGPFYDRPEQL
                     GGAAAVVPVDLFIPGCPPHPLTILDGLLRLLGRLEEEGRVAGR"
     gene            complement(9569..11092)
                     /locus_tag="PLP50_08705"
     CDS             complement(9569..11092)
                     /locus_tag="PLP50_08705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPA51669.1"
                     /translation="MSGAFVRIRNGGALPLAKLPEAGFDAFRGELLGELAAGRRLVAF
                     FAAPVDGAAAELWAVVAADGPGTLSIARTRLPDDRFPSLTPAAPQAHLFEREMAEQLG
                     LKPQGHPWLKPVRYRTSWRPGHDAWGRTEGDPILPAVGDFFRVEGTEVHEVAVGPVHA
                     GVIEPGHFRFQCHGEKVFHLEIALGFQHRGVEESLLGGPHRTTAARMETLAGDTTVGH
                     STAFAQALEALSGTAVPPRAQALRGIALELERLANHTGDLGALAGDVGFLPTASFCGR
                     LRGDFLNMTAVLCGNRFGRGLVRSGGTLVDADEALVADLSRRLEETMKDLRGAAELLF
                     ATATVTARFEGTGRVDPATAEALGLVGPAARACGVDQDVRRDHPSGVFRFVHVPVMTA
                     HDGDVNARAYVRWLEAQRSYDFVRAQLAALPEGSVRNAPKPLRPEHVAVSLVEGWRGE
                     VAHVAVTDAGGRFSRYKVVDPSFHNWTGLAMALRNEEISDFPLCNKSFNLSYCGHDL"
     gene            complement(11089..12522)
                     /locus_tag="PLP50_08710"
     CDS             complement(11089..12522)
                     /locus_tag="PLP50_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPA51670.1"
                     /translation="MEVGLVLLPLLGAVVAALVRSPERRAWVVPAAGVLHLLLLIGLV
                     LGNVRAGRGFWLRLDPLATVILGTISVLFVVAAFYAPGYLALRRDRDNRVFCSGLLVF
                     LAMTSLLALAQHLGLLWVAMEATTLATAPLLYFNRTARSLEATWKYLMIGSVGIAIAL
                     LGSLFLAYSAHVGGAEPSLLFDDLLAGAGSFSRPWLRAAFVLLLVGYGTKMGLAPLHT
                     WKPDAYGEASGIVGALLAGGMTNAAFLALLRVYRVVNAAGEGAFARELLVFMGLVSIA
                     LAAVFVVRQRDLKRMLAYSSVEHVGILVLGIGLGGIGVFGSLLHAINNGLTKGVLFLS
                     VGNIHRAFGSKHLDEVSGAISRLPVSGSLFLVGFFAITGSPPFGPFVSEFTIVNAAIG
                     GGHAVAGTLLLVLLGIVFIGMGSTVLSAVQGRPGTASAGTPYKDDLARTAPILVAAAL
                     VLLLGLWLPEPLVSLLERAAEGLEARP"
     gene            complement(12536..13177)
                     /locus_tag="PLP50_08715"
     CDS             complement(12536..13177)
                     /locus_tag="PLP50_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPA51671.1"
                     /translation="MTHPVDILLVLVILLNFLVLGTPSLRMTIRGAALQGVLLALLPL
                     LVHRGFGWRVGAIAVATMALKGLLIPGLLEKAMRDVHIRREVEPYVGFVPSLFLCAVG
                     TALAILFSGNLPLAPEHAGTLLVPASLATLLSGFLVLTTRKKAISQVVGYLILENGIF
                     VFGQLLIEAMPFLVEAGVLLDLFVGIFIMGIVINHIRQEFSSLDTELLSELKD"
     gene            complement(13182..14084)
                     /locus_tag="PLP50_08720"
     CDS             complement(13182..14084)
                     /locus_tag="PLP50_08720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPA51672.1"
                     /translation="MIDTLVHLALVLALPPLLTGVIAKTKAAFAGRNGPPLLQGYYDL
                     AKLLRKGSVLPAGSTWVFLAGPVAGVAAPLLASLLLPFGGHEAPLSFAGDMILFAYLF
                     ALARFFTASAALDTGSAFEGMGAAREVAFSALAEPALFFGFVALARISGSLSLSGMLG
                     SGPGWVTAGAPLVLVAASWAVVLLAENCRIPFDDPNTHLELTMIHEVMVLDHGGPAFG
                     LVLYGAAVKLFVLSALFLRVVFPFNATDPNLDWLAFVAGVLLVAIGIGVVESVMARLK
                     LVKVPQLLVGACLLSAFGMLLLAR"
     gene            complement(14099..16081)
                     /locus_tag="PLP50_08725"
     CDS             complement(14099..16081)
                     /locus_tag="PLP50_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPA51673.1"
                     /translation="MSLLLVLGGALLCAAGGVPGLLLRRAETGRRLALAGSLAGSLLG
                     LAGAALAAVTGTVETIQRSWPLPGAAFHVSLDALSALFLAPLFLVSAAAAVSGARSFG
                     DEAHPDDAPRTRFFFGLSTGAIALVLVARNLLLFLAAWEVMALAAFFLVTADRESREA
                     RDAGRLYLFATHVSTLVLFGFFALFRSASGSTELVPLPAGVAGSDAGTALFVLCLLGF
                     GLKAGLLPLHIWLPPAHAAAPAHVSALMSGVLIKTGIYGIVRFTSLVPDPPASWGDAL
                     FLLGAASAVLGVAFALGQHDLKRLLAWHSVENIGIIVLGLGVGLGGRAAGRPEWALLG
                     FAGGLFHVVNHGLFKPLLFLGAGEAAHVAGTRDMDRMGGIARVLPKTALLFVAGAVAI
                     SGLPPLNGFASEWLVYLGLFDRARATGADALRAAFGVPALALTGALALACFVKAHGAV
                     FLGNARGEAPQLHGQPASHGEPPAHVGPMALLAAACALLGLLPAVVAPALARAAAVAA
                     PGPVPAKPLAELASLGTVGVAALAVVLLVAAVALLLRRRLAAETTAGPTWDCGYASPT
                     ARMQYTASSFARGPVGWFGWALRPRLKGAPVAKLFPASARFESHVLDTVLDRAVVPVF
                     RAGAWLARQGRVLQHGKMQLYLLYVVATLVALLFRV"
     gene            complement(16078..18129)
                     /locus_tag="PLP50_08730"
     CDS             complement(16078..18129)
                     /locus_tag="PLP50_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPA51674.1"
                     /translation="MEAASPDGLLLAGAIALVALSGLPGLLARKGGSGGERLSALLVG
                     AGALLGLAASARVLFLGGGFRVELPSPLPGGPLLLSADALSALFLVPVFLVPALGAVY
                     GLGYWPEAEHRRDARKLRFFYGLLAASMAVVLLARSGVLFLVAWEVMALAAFFAATTE
                     DRQPSVREAGWIYLVATHAGTLGLFGMTALLARTTGGFAWEAPDGGLAVTASSSVVFL
                     LAVVGFGGKAGLFPFHFWLPGAHGGAPSHVSAVMSGVMLKVGLYGLLRVVSLYGAPPA
                     WWGGLLLLLGAGSALFGAALAAGQGDLKRLLAYSSVENVGIVTTGIGLALLGRSEGRP
                     EWVALGLGGALLHVLYHSLFKPLLFLGAGAVIHAAGTREVDRLGGLLRKMPRTGAAFA
                     AGCAAALAFPPLAGFFSELVVYVGLFRAFAGPDSSLLAAFAAPALAVAGAVALAAFTK
                     LFGVVFLGSPRSEEAAHAHEPGRAMLGPILVLGALVAVAGLLSPLLAPGVDAAIRAWV
                     APAVPPGSVGELAHPVPPLAAIVPLGTIALLGVAFLGLLALVAGLLALRLRRASFDRG
                     ATWDCGYARPTGRMQYTGSSFGDWIAGRLTPSAAAPVLEVRPPVGSFPRGAAVSAPAP
                     ARDPLLLRLLEPFASRWARRFHDLHALQEGRLTIYLVYVLGTLVALLAWSALRGWVLP
                     R"
     gene            complement(18129..18830)
                     /locus_tag="PLP50_08735"
     CDS             complement(18129..18830)
                     /locus_tag="PLP50_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPA51675.1"
                     /translation="MKLTTADAADLLDVDESTIYRWIEERDLPAERIAERTLLNRAAL
                     LEWATEHRVAVSPRVFLPAEAGGERLPSFAAALAAGGVHHALPGGTRQEVLREAVSRL
                     PLPEGIEAEELLAFLLARETAGSSAIGDGIAIPHVRAPIVLDVDEALVSISFLAAPVD
                     FGASDGRPVTVLVTMVTPTVHGHLHLLAALACLLRDEAFRAAVRAAAPADELLALARQ
                     IEARGGGRRAEAGAV"
     gene            complement(18827..19717)
                     /locus_tag="PLP50_08740"
     CDS             complement(18827..19717)
                     /locus_tag="PLP50_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51676.1"
                     /translation="MAFVGRRAARPLLPCLPALLLAPVLSAQAPLETIPDPARLAPAE
                     VPFVNPLWQALDAPAAPKGPPPRLRDLETTGPLIVGVKLDAAGAVSETVVPEPPLRAL
                     AAAVQAQAPRWTFQPATKDGVAVRCWGTYGVDVEVELEDATWHAFALVPVGKEDPLPE
                     LPKEMPGETWMSRYPAAVSPAEPGVLSVEEVDFLPMPVKVPLKLEGTRLKSRLLALVE
                     VSGLGTVKRVVPVGTYEPMILRWVRESAKEWALTAARTGGSPVTSWLALDTTIEYSLG
                     SVKERGKRSVKKNVRGEPVP"
     gene            complement(19738..22593)
                     /gene="polA"
                     /locus_tag="PLP50_08745"
     CDS             complement(19738..22593)
                     /gene="polA"
                     /locus_tag="PLP50_08745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPA51677.1"
                     /translation="MPEKKTLALLDASGYLYRAFHAIRPLTAPDGRPTNATFGFATML
                     RKLLSQRKPDAVAVCFDRPEKTFRHEMDPEYKATRAAMPDDLVPQVADVKALCRAMGL
                     VVVEEPGFEADDLIGTLAEKGARAGLAVEVVSADKDLFQLVREGEVAVWHPVHEKLLD
                     SEGVRELFGAPPERVTDVLGLMGDSSDNIPGVRGIGEKGAKELVATFGSLEEIYARVG
                     ELKGKRREALEAGKAEAFRSRELATVRRDAPTPGGDNVESLLGTFAVPPASPEGAAAL
                     AAFFGELGFSRLRRELLEAAAATAPPPAKAPKSAGSGSLFDTAGEVAAAPVSGAGEGT
                     GERDAEEAGGPAVTAGSASGDLPAWESTPAALDAFLARSEVAGRIGLHVECAPGRPFP
                     PEPIALAVAIPGGGALTAPLRGEGSEAGRALLARLLAHEGVELVAHESKRLHLAAAAL
                     SLPAPRRVFDAMLAAYVDSPGLHAHDLDSDARALLGRPPETVPSPKDVAGSDAFETAA
                     ELATEAGRAYLGPRARLPLELADALFPRLSARPASLRVYEELELPLVPVLARMERAGI
                     LVDPDALSTLSVDLGARLARLEAEIHAAAGEPFNVGSPPQLGRILFEKLGYPVLKKTA
                     KTKSYATGSEVLEELSLKGFGPLPGLVLEWRELSKLKGTYVDALPRHLAADGRIHTRF
                     DQAVAATGRLSSNDPNLQNIPVRTEAGRSIRKAFVAPQGRLLVSADYSQVELRLLAHL
                     SGDAALIETFRRGDDIHRATAARIFGIHPDLVSPDQRRGAKTINFGILYGMGPFALAG
                     QLGVTQGEAKAFIAAYFQQFPSIRACLDRILAQARETGSTETIFGRLRPIPGIADRNH
                     AVRANAERMAMNAPFQGSAADLIKKAMIDLEVRLTAEFPSARMLLQVHDELVLECDEG
                     DAGPVAALAKETMEDVASLAVPLTVETARGADWAAAK"
     gene            22648..23745
                     /locus_tag="PLP50_08750"
     CDS             22648..23745
                     /locus_tag="PLP50_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51678.1"
                     /translation="MSSVPIPPAPPVTNARVASALLALADAEPPGARPASEYRSAAAT
                     LRQLPDELHGRRIHLGGLPHVTPRAADAIAAFLSCGSDESVDDRLAALLDRDDPARER
                     KREFLSRADVGRILAAPEGISPADLRADVHLHTDRSDGQIPLADLHRALAARGDRYAL
                     LTDHARDCAVAGGLHAGDFAAQARELERLNARTGEEFGMLLGAEANIAEDGSIDVLPA
                     TVPSLAGVVASVHTDLRGPKDQTARLVRAIERPGVAILGHPKGRLYNMRTGVRASWDR
                     VFAAAAERGVAIEINGCPERQDLPPSLARTALAAGCLFALVSDAHAHRHLPFQRVSLA
                     IARLAGIPAERVVNTWPRERFEEWLEERRLG"
     gene            23811..24278
                     /locus_tag="PLP50_08755"
     CDS             23811..24278
                     /locus_tag="PLP50_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPA51679.1"
                     /translation="MGLFGKSLDEKAAEAVKSLRGSIKGLKSLDAVVEGKTVTLTGEA
                     DDLEAKGKAMAEFNKLVETENTINKIALTKAPVAAAPTPAAGAVVTGGAPAATAAEAA
                     AKVHVVEKGDTLGAIAKKYYGKAGAYMKIFEANKDVLTDPDKIKPGQKLRIPD"
     gene            complement(24345..25208)
                     /locus_tag="PLP50_08760"
     CDS             complement(24345..25208)
                     /locus_tag="PLP50_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPA51680.1"
                     /translation="MPVAPAEKVFDARTARVSAAVLAAVPLAGWITWWREEEGASFHF
                     REEGGLGRRSYAGGADKASHFVMAAIAQDGAESIYRSLGKDERTARLLGIALVSAAGL
                     VIEIGDGFTRYGASWEDAAANVLGAAAAAGVNGLRLEDTIGFRFGVVRARVPDPCCRA
                     TAYGGDYSRWVQSVDLKLDGLLPRLGVAPGPARFLLVSLTYGSKGYRFSPVEVRQRNV
                     GIDLGLNVEQVLRAVGVRDESWWGRTLLFLSRTYRIPWTAFGWRYDLNAGRWHGPDTG
                     DRFDPGAVIYD"
     gene            complement(25412..>25984)
                     /locus_tag="PLP50_08765"
     CDS             complement(25412..>25984)
                     /locus_tag="PLP50_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPA51681.1"
                     /translation="PGGGSASAAAGAMGAALLGMVCRLTLGKEKYRESWEELERVASA
                     MDANRDTLLGLVDEDTRAYDAILAARKLPKETPEEKAARKKAIDEATILATTVPMQTA
                     FFSMEALAKAPVVLEKGNPNCASDAWVAALLLSAALEGALANVRINLPGISDAGLAEG
                     FRSDAEQAAKKAAETMERVRTLAKAKELAG"
BASE COUNT         3769 a         9299 c         9888 g         3028 t
ORIGIN      
        1 tcttcttcag gagcttgccg acgtggaact tcaccgtcgc ctcggtcacg ccgagccggc
       61 gcgcgatctc cttgttcgga agctcgtccc gcaccgcctc gaggacccgc tgctccgacc
      121 gcgtgaaggc gctgacgtgc ccgaggccga ggcgcgagac ccgctcgacc cactcggaga
      181 ggacggcccg cggcgtccag agcgtcccct cgcgaacgca gcgcagggcc tggcgaagct
      241 cggccgggcc tgcggtgtgc ggcagcacgc ccttgacccc cgccatcagg caggggaacg
      301 tcgtccgcgc gttcacctcc cggacgacga cgaggacctt cgcctccgga tggcgcaggc
      361 ggacccgctc gattcgaggg gtcggcgagc cgggctcgtc gccgagaccg aagaggatga
      421 gatccggctc cgggcgcccc tcgccgtcct ccgtggagac gtcgtagccc tcggccacga
      481 ggaacgggcc gaggctctcg cgccgccacg ggtcgctctc gagaaggagg aggacgggcc
      541 ggccgcggcg gatcacgcct tgctccgcgc gccgcccggg cgtgacgcca gcgtgatacc
      601 cgcccctcgg ggcattttcg gcactcgaat cctccgcact ccgaagaccg tcacagtcta
      661 gcgccttcgg agggccggca cgcccccgac gcggcccctt ccttcgcgcg atcctcatcc
      721 gggggctcgg ggcactgaca gtcttctctg tcacgacaac cccggatcgt cggagcggtc
      781 gaggccgcct cgttccgcgc tcgtcggctc gctccccgtc gctggccgac aggccttcct
      841 gtcggtcgag tgcgcggagc gcgacgcgct ctacgcgcaa gcgattgtca cggatcgact
      901 tgactcccga aggcggccct cggccgggcg cttgcagcgt ccggagcaga cctcgagaca
      961 cccaccagga gaggaggaga ggaatggcgc aggagacctg accggccgcg gccctccgca
     1021 cgaagaagag acagacgacc gaagccccga ccgaagcgcc ccgatccggg gcagatcccc
     1081 aagaaagaag agaaggagac cacgatgttc gctcaccacg ctgcccgcac gttcgccctc
     1141 accctcggcc tcgtcgccct cctcgccagc gcctcctccg cccccgcggc ggaggagtcg
     1201 accgccgccg cgaagaagag cgtcgtcaac ctgaaccagg cgtccgccga cgagctcgcg
     1261 cgcctgccgc gcgtcggccc gtcgctggcc gggaagatcg tcgcccaccg ggagcagcac
     1321 ggtccgttca agcgggcgca ggacctgatg gaggtgaagg ggatcgggga gaagatgttc
     1381 gcgctcctcg agccgtacct cgccgtctcg ggccccacga cgctgacgga aaaggtctcc
     1441 tcgaagggaa cgaccgcccg gaaggcggcg aagcccgcga aggcgaaggc ccccgccgtc
     1501 gcggagaagg ccggccgctg aggcgccgcg cgggccgggg gtcttccccc ggcccgcgcc
     1561 cgtcttcgag acgactcgag gctgcgaggt gtctccgtgc cgcccgcccg acgctccctc
     1621 cgcgggatca ccctccccga gatcctgacg accctcgcga tcctctccct ggccgccgga
     1681 ctctcggcgg cggccgctca cggcctcgcc ggccagacgt cgctgcgcgc cgcggcccac
     1741 gaggtcgcct ccgtcttcac gcaggcgcgc ggccgcgcga tccaccgggg gatccagtgc
     1801 ggcgtgaagt ggatcgcccg cgacggcgac ctgacgctcc agattcacga cgacggggac
     1861 ggcgacggcg tgaggagcga cgacatcgag ggcggcgtcg acccgctcgt ctacggcccg
     1921 gtctcggtga agaagcgctg gccgaaggtg acggtcggct tcatccccgg gttcgtcgcc
     1981 cgggatccga aagggaatcc cgtgggcgat ctctccgacc cggtccgctt cggccgctcc
     2041 gacatcgcga gcttctcgcc gctcggcgac tgctctcccg gctcggtctg gctgggcgac
     2101 gcgaggagcc ggcagtcgct cgtccgcctt tccccgacga ccgcgacgat cgcgatctac
     2161 gagtgggccg cggcgcggca gagctggatt cgcatctggt gacggccctt ggcggggatc
     2221 gccgcgtcag gcgaggcccg acgcggcgat cgcctcctcg gccgcggcga gcttcgccgg
     2281 ggagccgagg tcgaaccaga ggccgtcctc cgggacgcga tacgggacga gcgcgaaggc
     2341 gccgtcccgt cgctccgtcg aggggcggag aacgtcgcgt gcgagctcgg agacgccggg
     2401 cgggatccgc gggacgagcg cgggcgcggc gcactgcagg ccggtgaaga gccacggtcc
     2461 cgtcgcctcg ggaaacggcc gcacgtcgcc gagggagacg agccgcccgt ccgcgtcgcc
     2521 ccagagcgcc gtctcgcccg gcgcggcgtg cgggaggacg ccgagcgccg agacgacgcc
     2581 gtgcgcgtcg agcgccgcca gaagcggggc gaaggggagc gtcgtgaaga ggtctccgtt
     2641 gacgacgagg aaccgcccgt cgccgaggag gccgcgctcg gaggcgcgcc ggatcgctcc
     2701 cgccgtcccg aggatcgggt cttcggtcga gaaggcgacg gggatcccct cgcaggaatc
     2761 gccgaccgcc gccatcagcg tctcggcgag gtggtgcgcg ttgaggaccg ccgaggtgac
     2821 gccctgcgcc ttcaggtgcc ggaagagccg gacgaggatc gggacgccga ggagcgggag
     2881 gagcggcttc ggcgtggtga gcgtggcggg gcggaagcgg gtgccgaagc ccgcggccgg
     2941 gacgagcgcc ttcattcgcc gaagaccatg tcctcgggaa ggtcggggtc gacctcgcgg
     3001 gccgcctgcc agagcccgaa gtacgaggcg cgcaggaacg actcctcggc gacgccgagc
     3061 tccgcggcca gggcgcggac ggtccccgac tctccctcga tctcggcgaa gaggccgagc
     3121 ggcgtctcgt cgacgacgac gagggggcgc gcgggaccgg cgaagcgcca cggcgtccgc
     3181 cgcttgtcgt accggaagcg gggacggaag ccgagcgcct cgaggagggc gaggacgttg
     3241 cccggcgcgt cgacgccgct ctccagctcg agccttccct tgacgccccc gtcgaaggaa
     3301 gccggcccct tgtacgtgag gaggccgcgg cccccgaccg tacggacgcg cagcgcgcac
     3361 ccccgcgagc ggagcgaacc gtcgtcgtcg tcgaggagga ggttgacctc gtcctggcgc
     3421 gccccgggga cggcgccgag ggcggcgagg cgctcctcga gcgccggccg gtcggcgacg
     3481 cggaacttcg cctcggtttc gcggggggcg tcgctcacgt ccctagaata gcggccgtgc
     3541 cgcgctaccg cctctcgggc ctccgtctcc gcgcgccgca ggcgcccgac gggacgctcc
     3601 tcgcccgcct cctcggcgtc ccgccggggg acgtctcggg cgtggaggtc gtccgccgtt
     3661 cgctcgacgc gcggaagaag cccgacctcg tctacgagta cgcggtagag ctgacgctcg
     3721 cttcggaacc cgccgctccg gccgccccgc cgctcgctct cgagcccgct ccggagccgc
     3781 gcgcccccga gctcctcctc ccgcccctcg aacggaaggc gcgcgtcgcc gtcgtcggga
     3841 ccggcccggc cggcctcttc gccgcgctcg ccctcgccga cgccggggtc tccgtcgtcg
     3901 tcctcgagcg gggcgacgcc gccgaagagc gctaccgccg cgtgatgcgg ttctggaagt
     3961 cgggcgactt cgacccggag tcgaacgtcc agttcggcga ggggggcgcg gggacgttca
     4021 gcgacggcaa gctgacgtgc gggaagcggc acgacaagat cgccgtcgtc ctcgagacgc
     4081 tccatcgctt cggcgcgccc gacacgatcc tctacgacgg caagccccac atcgggaccg
     4141 accggctcgt cgtcgtcctc cggaacatcc ggaagcacct ccgcgagcgg ggcgccgagc
     4201 tgcgctaccg cgcgaaggtc gtcgacgtgc tccgcgaagg cgaggacggt ctcctgcgcg
     4261 cgctcctcct cgagagcggc gaggaggtgc cgtgcgacgc ggcgatcttc gcgctcggcc
     4321 actcggcgcg cgacaccgtc gagcggctcc tcgcccgcgg cctcgcgatg tcgccgaagg
     4381 cgttcgcgat gggggtacgg atcgagcacc cgcaggacga cgtcgaccgg atccagtacg
     4441 ggcggctcgc cgcctcctgc ggcgtcatgc cggccgacta caagatgacg gcccagacct
     4501 ccgtcgggcg cgccgtctac acgttctgca tgtgtcccgg cggcgaggtg atcgcctgct
     4561 cgtcggagcc ggagggcgtc gtgacgaacg ggatgtcgcg ctacaagcgc gcgtccggct
     4621 tcgcgaactc gggcctcgtc gtccagacgc acctcgggga cttcgcgggc gagagcgggc
     4681 ccgaggtcct ccgcggcatc gcgttccagc ggcgggtcga gcggaccgcg ttcgcggcgg
     4741 ccggcgggac gtacgccgcc ccggcaatcc gcgtgacgga cttcctttcc ggacgcgcgc
     4801 cgcgccccct cccgaggggg acctacacgc cgtcctgcgt cccggcccgg ctcgagggga
     4861 tctacccgga ggcgtacctg acggctctcg ccgaggcgct ccgctccttc gagaggaaga
     4921 tgcgcggctt cgtctcggac gaggctgtcc tgatcgcccc cgagtcgcgg acgtcgtcgc
     4981 cggtgaggat cgaacggaac gaggcgtgcg aatcgccttc gctccccggc ttcttccccg
     5041 cgggcgaagg ggccggcttc gcgggcggga tcgtctcggc ggcgctcgac gggctgcgcg
     5101 tggcgcgcgc ggtacgggcg cggctggccg gcgaggtgcc gccgccgtcc tcggcccggc
     5161 cggtcgaagc gcccgagtac tgaagcgcta cgtcggacgg gtcgtgtcgg gcgtcgccct
     5221 tcccgcctct tcctcgtgcg ccttcaggat ctcgcgctcg agcgcctcct gccgcgagcg
     5281 ggcctcgccc ttcccccgcg cgcggagcgg gttcgccgcc gcgcgcgcga ggacctcctc
     5341 ccagccgaac gggaccgggg cgtcggcctc gtcgccgagc tcttcccact cggcgtccga
     5401 gagcggcgcg cggagcgcga ccggctcgag ccgctcgccg gtccgccgga tggagaggac
     5461 gtgccggctc tcctcgccgg tcgcgatctc gaacgaggtc gccgggaagt cgccggagag
     5521 ctccgcttcc tcgaggtcgg gctgggcgag gagcacgacg gttccgtccg tcagcttcgc
     5581 gaaccaggtg gtcgagacga agcggcccgg atcggaggcg accttcacga acgcgtccgc
     5641 ctcccagcgg ttgacgacga cgcggcccga ttcgaggtcc cgctgcagga accccaggac
     5701 gggctcggcg gggcgggccg actcggcgtg ctgcgcggac agccgcttcc cgaagtgcca
     5761 ggccccgccg ccggcgatcg cgacggcgag gaggagccgc accgcggccg ggacgaggcc
     5821 gccgaggagc atgttcagaa tcatgaagag ggcccacgcg agcggcacgg tgaccatcac
     5881 ggtcgcggcc cacgccgggt tcgacttcgg cttcggatgc aggtacgcct cgagccgcag
     5941 gcgctcctcc ggcgtggccg ggcgggtcgt cgtcgccttc atgagcaccg gatgatgcca
     6001 cctcgctccc gcttcccggc gccgtccgtg acgcccctcc cgcgatcccc gatactcccc
     6061 gcatggcgcg cgtcctcgtc ggcaccgcgg gacacatcga ccacggcaag agcgccctcg
     6121 tccgggccct gaccgggacc gacccggacc gcctccccga ggagaaggcg cgcgggatca
     6181 cgatcgacct cggcttcgcg cacgcggcgt ggggcggcac gacgttctcg ttcgtcgacg
     6241 tcccgggtca cgagaagctc gtccggacga tggtcgccgg cgccgccggg atcgacgtcg
     6301 cgctcctcgt cgtcgcctcc gacgactccg tcatgcccca gacgcgcgag cacctcgcga
     6361 tcctctccct cctcgacgtg aggtgcggcg tcgtcgcacg gacgaaggcc gacctcgtcg
     6421 acgaggagac gggcgccgtc gtcgacgccg agatcaccga cctcgtctcc gggacctttc
     6481 tggaaggcgc tcccgtcgtc cccgtctccg ccctcaccgg gcgcggcctc gacgcgctga
     6541 gggatgccct cttccgggag gcctcggccg ccgacctccg cgacccgtcg gcccggccgg
     6601 cgcgcctctt catcgaccgg gtcttcacga tgaaggggtt cgggccggtc gtcaccggca
     6661 ctctcgattc cggccgagta cgggtcgagg accgcctgac gctcctgccg gagggacgcg
     6721 agctgcgcgt ccggcgcctc gaggtgcacg gcgaggagcg cgaggcggcg gaggccgggg
     6781 agcggacgag cctgaacctg gccggcatcg agacctcgga gctgggccgc gggatggccc
     6841 tcgtcgcccc cggaagcctc gcggtatcga cgctcctgac cgtcgaggta acggcccttc
     6901 ccgacctcga cgatccgttg tcggacggcc tttccgtccg ccttcacatc ggcaccgccg
     6961 acgtcgcggc gcgcctgcac ctcctcgacg gaggcgccgg cgccccgtcc ctccctccgg
     7021 gcgagcgggt cgtcgctcag gtctcggctc tcgagcccgt cgccgctcgc cgaggcgacc
     7081 gcttcgtcct tcggcggccg tcgccgcagg agacggtcgg cggcggccgg gtcctcgacg
     7141 tcgggcgccc gcgcgtgaga cggcgcgcgc cgctctccgc tcacgcggcc gccgtcctcg
     7201 ccggggacga cgagcgcgcc gtcgcggtgc tcctcctctc cgaggccggg cctgccggcc
     7261 tcgagatcgc cgagctcgcc aaccggctcg gcgtcccgga cgccgccgcg gccgcgcacg
     7321 tttgcgcgct cgtcgaaagc ggcgcggcgc ttcggctcac gcccgctctc gccgtcgccg
     7381 cgtcggcctc ggtcgagatg gcgacccgcg ccgacgcctt cttcgccgag cggcggaagg
     7441 ccggggtccc gacgctcctc gtcgggcggc gcgagctcct cgggaagatc gcgcgcggcc
     7501 tcccggagac gacggccgag gcgtggctcg cgacgctcgc cgccgcgaag cggctcgtcg
     7561 tgaccggcga ccaggccggc ccgcccggcg ccgccgcctc gggcgtggcg gacgaggccg
     7621 gggccttcgc ctcccgcatc gaggaggcgt ggcggacggc ggggttcgac gcccccggca
     7681 acctcgacct cgcgaaggcg ctcggcacga agccgcaggt cgtcgccggg ctcgtccagc
     7741 acctcctcaa gagcggggcg ctcgtccgcc tctctccgga ggtcttcgtc cacgcggagc
     7801 ccctcgccgg gatcgaagcg aaggtggcgg agcagaaggg gcggacgatg agcgtcggcg
     7861 acttccgcga cctcctcggc ctgtcgcgga agtacctgat cccgctcctc gagcacctcg
     7921 accggaagaa ggtcacccgg cgcgtcggcg acgcgcgcct cgtcgtctga cgggaggggc
     7981 acgccgagcg cggacggggc gcgaatggcg ctttcgtgag tatggccata ccattacggc
     8041 atggccacca cgaccgtgaa ggcgacctac tcgctcgatc ccgggacggt cctccggctg
     8101 gaggagatcg cgcgcagctg gggcgtctcg aagtccgagg cccttcgccg ggcgatccgc
     8161 gccgcggcgt cgggcgaggc ccccgcgagc ggagggccgc tcgacgcgct cgacgccctg
     8221 cagcaggcgg ccgcgctcac accagcatcg gctcgcgcct gggccgccga cgcccgttcg
     8281 gaacgccgcg ccccgcgcgc gcaggccggg aagtgctccc ggtgacccac ctcgacacga
     8341 gcttcctgat ccgggcgctc gtgccgggaa gcccggaaga cgcgacgctc cgcgcctggc
     8401 tccgtcgcgg tctcccggtc gcggcgagcg cggtcgtctg gaccgagctc ctctgcgggc
     8461 cgctgacggc gcgagaggcg gagctcgccc tcctcgtcgt cggagagcct gtcccgttct
     8521 gcggggagca tgcggcgctc gccgcgtcac tcttcaacgc caccgggcgc agacgcgggt
     8581 cgctcgcgga ctgcatggtc gccgcggccg cgatccggtc cgaggccgag ctcgcgacgg
     8641 cgaacgaggc ggatttcgcc cggttcgtcc cgctcggtct ccggctggcg ccggcggaat
     8701 ccgctcggcc ggcgcgtcca cgtccccgga agtgaccatc cagagctcga gcgtcgggcc
     8761 ggttcttccg ggtcagcgac ccgccacgcg cccctcctcc tcgagccgtc ccaggaggcg
     8821 aaggaggccg tcgaggatcg tgagcgggtg gggcgggcag ccaggaatga agaggtcgac
     8881 cgggacgacg gcggccgcgc cgccgagctg ctcgggacgg tcgtagaacg gcccgcccga
     8941 gatcgcgcag gcgccgacgg cgatgacgat cttcggcggc ggcaccgcgt cccacgtctt
     9001 gcggagcgcg agctccatgt tcttcgtgac gggcccggtg atcaggactc cgtcggcgtg
     9061 ccgcggcgag gcgacgacct ggatgccgaa gcggccgagg tcccagccga tcgtcccgag
     9121 gacgttcacg tcggcctcgc aggcgttgca gccgccagcg ctcacgaccc ggagcttcag
     9181 cgaccgtccg aagagcgaga ggagtctccc ctcgagcgcc cgcgcgagcg ggagcgccgc
     9241 gccgtcgagc gtgaggtcct cgcgggtgcg gacggcgagg cggtagtcgg aggtgaagga
     9301 gatcgccttc tcggggcagg cggcctcgca ctcggggcag aagaggcagc ggccgaggtc
     9361 gagcgacggc ttcccgccgg cgatcgcgat cgcctccgtg gggcagacgt cggcgcaggc
     9421 gcggcagcct tcggggcagc gggacgcgtc gatcgcgggg aggccgcggt agcgggacgg
     9481 gagcgccggc ggcgggccgt cgggccaggc catcgtccgg tgcttctggg agatccgctc
     9541 gaggagggcc ctgatcatct ccgcctcctc acaggtcgtg cccgcagtag gagaggttga
     9601 agctcttgtt gcagagcggg aagtcggaga tctcctcgtt ccggagggcc atcgcgaggc
     9661 ccgtccagtt gtggaacgag gggtcgacga ccttgtagcg cgagaagcgg ccgccggcgt
     9721 ccgtcacggc gacgtgggcg acctcgccgc gccacccttc gacgagggag acggcgacgt
     9781 gctcgggccg aagcggcttc ggtgcgttcc gcacgctccc ttcggggagc gcggcgagct
     9841 gcgcccggac gaagtcgtac gagcgctgcg cctcgagcca gcgaacgtag gcgcgcgcgt
     9901 tcacgtcgcc gtcgtgcgcg gtcatgacgg ggacgtggac gaagcggaag acgccggagg
     9961 ggtgatcgcg ccgcacgtcc tggtcgacgc cgcaggcgcg cgcggccggc ccgacgaggc
    10021 cgagcgcctc ggccgtcgcc gggtcgacgc gccccgtccc ctcgaaccgg gccgtcaccg
    10081 tggccgtcgc gaagagaagc tccgcggcac cgcgcaggtc cttcatcgtc tcctcgagcc
    10141 ggcgcgagag gtccgcgacg agcgcctcgt cggcgtcgac gagcgtcccg ccggagcgga
    10201 cgaggccgcg gccgaaccgg ttgccgcaaa ggacggccgt catgttcagg aagtcgccgc
    10261 ggaggcggcc gcagaacgag gccgtcggga ggaagccgac gtctcccgcg agggcgccga
    10321 ggtcgcccgt gtggttcgcg agccgctcga gctcgagggc gatgccgcgc agcgcctgcg
    10381 cgcgcggcgg gacggcggtc cccgagagcg cttcgagcgc ctgggcgaag gcggtcgaat
    10441 gcccgacggt cgtgtcgccc gcgagcgtct ccatccgggc ggcagtcgtc cggtggggcc
    10501 cgccgaggag cgactcctcg actcctcggt gctggaatcc gagggcgatc tcgaggtgaa
    10561 acaccttttc gccgtggcac tggaagcgga agtgccccgg ctcgatgacg ccggcgtgga
    10621 ccgggccgac ggcgacctcg tggacctcgg tcccctcgac gcggaagaag tcgccgaccg
    10681 cggggaggat cgggtcgccc tccgtccgcc cccaggcgtc gtgccccggc cgccacgagg
    10741 tgcggtatct caccggcttc agccacgggt gcccctgcgg cttcaggccg agctgctcgg
    10801 ccatctcccg ctcgaagagg tgcgcctgcg gcgccgccgg ggtgagcgag gggaagcggt
    10861 cgtccggaag acgcgttcgc gcgatcgaga gcgtcccggg gccgtcggcg gcgacgacgg
    10921 cccagagctc ggcggccgcg ccgtcgacgg gagcggcgaa gaacgcgacg aggcgcctgc
    10981 ccgccgccag ctcgccgagg agctcgccgc gaaaggcgtc gaagccggcc tcggggagct
    11041 tcgcgagcgg gagagcgccc ccgttgcgaa tacggacgaa ggcgccgctc acggccgcgc
    11101 ctccagcccc tcggccgccc tttcgaggag ggagacgagc ggctcgggga gccagagccc
    11161 gaggaggagg acgagcgcgg cggcgacgag gatcggggcc gtccgcgcga ggtcgtcctt
    11221 gtagggcgtc ccggccgagg cggtgccggg ccgcccctga acggccgaga ggaccgtcga
    11281 gcccatgccg atgaagacga tgccgaggag gacgaggagg agcgtcccgg cgaccgcgtg
    11341 ccctccgccg atcgcggcgt tgacgatcgt gaactccgag acgaacggcc cgaagggcgg
    11401 ggagccggtg atcgcgaaga agccgacgag gaagaggctt cccgagaccg ggaggcggga
    11461 gatggcgccc gagacctcgt cgaggtgctt gctcccgaag gcgcggtgga tgttcccgac
    11521 ggagaggaag aggaccccct tcgtcaggcc gttgttgatc gcgtgcagca gcgacccgaa
    11581 gacgccgatc ccgccgaggc cgatgccgag gacgaggatg ccgacgtgct cgacgctcga
    11641 gtaggcgagc atccgcttca ggtcgcgctg cctcacgacg aagacggcgg cgagcgcgat
    11701 cgagacgagc cccatgaaga cgaggagctc ccgggcgaag gccccctccc ccgccgcgtt
    11761 cacgacgcgg tagacgcgga ggagggcgag gaacgcggcg ttcgtcattc cgcccgcgag
    11821 cagcgccccg acgatgccgg aggcctcgcc gtaggcgtcg ggcttccagg tgtggagcgg
    11881 cgcgaggccc atcttcgtcc cgtagccgac gaggaggagg acgaacgcgg cccggagcca
    11941 cgggcgcgag aaggagcccg cgcccgcgag gaggtcgtcg aagaggagcg acggctcggc
    12001 gccgccgacg tgggccgagt aggcgaggaa gagcgagccg aggagcgcga tcgcgatgcc
    12061 gaccgacccg atcatcaggt acttccaggt cgcctcgagc gagcgggccg ttcggttgaa
    12121 gtagaggagc ggcgccgtcg cgagcgtcgt cgcctccatc gcgacccaga ggaggccgag
    12181 gtgctgcgcg agggcgagga gcgaggtcat cgcgagaaag acgaggaggc ccgagcagaa
    12241 gacccggttg tcgcggtcgc gccggagggc gaggtagccc ggcgcgtaga aggcggcgac
    12301 gacgaagagg acgctgatcg tcccgaggat gaccgtcgcg agcgggtcga ggcggagcca
    12361 gaagccgcgc ccggcgcgga cgttcccgag gacgagaccg atcaggagca ggaggtggag
    12421 gactccggcg gccgggacga cccaggcccg ccgctcgggc gagcggacaa gcgccgcgac
    12481 gacggcgccg aggagcggga ggaggacgag gccgacttcc atgcgcgcct ccgcctcagt
    12541 ccttcagctc cgagaggagc tcggtgtcga gcgacgagaa ctcctgccgg atgtggttga
    12601 tgacgatccc catgatgaag atgccgacga agaggtcgag gaggacgccc gcctcgacga
    12661 ggaacggcat cgcctcgatg aggagctggc cgaagacgaa gatgccgttc tcgaggatga
    12721 ggtagccgac gacctgcgag atcgccttct tccgagtcgt caggacgagg aagccggaga
    12781 ggagcgtcgc gagggaggcg gggacgagga gcgtcccggc gtgctcgggg gcgagcggga
    12841 ggttccccga gaagaggatc gcgagggccg tcccgacggc gcagaggaag agcgacggga
    12901 cgaagccgac gtacggctcg acctccctgc ggatgtggac gtcgcgcatc gccttctcga
    12961 ggaggccggg gatgaggagc cccttcagcg ccatcgtcgc gacggcgatc gccccgactc
    13021 gccagccgaa gccgcggtgg acgaggagcg ggaggagagc gaggaggacc ccctggagcg
    13081 ccgcgccgcg gatcgtcatc cggagcgacg gcgtcccgag gacgaggaag ttcaggagga
    13141 tgacgaggac gaggaggatg tcgaccgggt gcgtcatccg ttcacctcgc cagcagcagc
    13201 atgccgaagg ccgagaggag gcaggccccg acgaggagct gcggcacctt cacgagcttc
    13261 aggcgcgcca tgaccgactc gacgacgccg atgccgatcg ccacgaggag gaccccggcg
    13321 acgaaggcga gccagtcgag gttcgggtcg gtcgcgttga aggggaagac gacgcggagg
    13381 aagagcgccg agaggacgaa gagcttcacc gccgcgccgt agaggacgag gccgaacgcc
    13441 ggcccgccgt ggtcgaggac catcacctcg tggatcatcg tcagctcgag gtgggtgttc
    13501 gggtcgtcga acgggatccg gcagttctcc gcgaggagga cgacggccca gctcgccgcg
    13561 acgaggacga gcggggcgcc ggccgtcacc cagccgggac ccgagccgag catcccggag
    13621 agggagaggg agccggagat ccgcgcgagg gcgacgaagc cgaagaagag ggccggctcg
    13681 gcgagggcgg agaacgcgac ctctcgcgcg gcccccatcc cctcgaaggc cgacccggtg
    13741 tcgagcgccg cggaggcggt gaagaagcgg gcgagggcga agaggtaggc gaagaggatc
    13801 atgtcgcccg cgaaggagag cggcgcctcg tgccccccga acggcaggag gagggaggcg
    13861 aggagcggcg ccgcgacgcc cgcgaccggc ccggccagaa agacccaggt cgaccccgcg
    13921 gggaggacgc tcccctttcg gaggagcttc gccagatcgt agtagccctg caggagcggc
    13981 gggccgttcc ggccggcgaa ggcggccttg gtcttcgcga tgacgcccgt caggaggggc
    14041 gggagcgcga ggacgagggc gaggtggacg agcgtgtcga tcatcggatc tcccggggtc
    14101 agacccggaa caggagcgcg acgagcgtcg cgacgacgta gaggaggtag agctgcatct
    14161 tcccgtgctg gaggacgcgc ccctgccggg cgagccacgc tccggcgcgg aagacgggga
    14221 cgacggcgcg gtcgaggacg gtgtcgagga cgtggctctc gaatcgcgcg gaggcgggga
    14281 agagcttcgc gacgggcgcc cccttcaggc gcggccgaag ggcccacccg aaccacccga
    14341 ccggcccgcg ggcgaacgac gacgccgtgt actgcatccg cgcggtcggg ctcgcgtagc
    14401 cgcagtccca cgtcgggccg gccgtcgtct ccgccgcgag ccggcggcgg aggaggagcg
    14461 cgaccgcggc gacgaggagg acgaccgcga gggccgcgac gccgaccgtc ccgagcgagg
    14521 cgagctcggc gagcggcttc gccgggacgg ggccgggcgc ggcgaccgcc gcggcgcggg
    14581 cgagggcggg agcgaccacc gccgggagga ggccgaggag ggcgcacgcc gcggcgagga
    14641 gcgccatcgg gcccacgtgg gccggcggct caccgtgcga cgccggctgg ccgtggagct
    14701 gcggcgcctc gccgcgcgcg ttcccgagga agacggcgcc gtgcgccttc acgaagcagg
    14761 cgagcgccag cgcgccggtc agggcgagcg ccgggacccc gaacgccgcg cggagggcgt
    14821 cggccccggt cgcgcgggcc cggtcgaaga ggccgaggta gacgagccac tcgctcgcga
    14881 agccgttcag cggcgggaga ccggagatcg cgacggcccc cgcgacgaag aggagcgccg
    14941 tcttcgggag gaccctcgcg atgcccccca tccggtccat gtcgcgcgtc ccggcgacgt
    15001 gggccgcctc ccccgccccg aggaagagga gcggcttgaa gaggccgtgg ttcacgacgt
    15061 ggaagaggcc ccccgcgaag ccgaggagcg cccactcggg gcgccccgcc gcccgcccgc
    15121 cgaggccgac cccgaggccg aggacgatga tcccgatgtt ctcgacgctg tgccaggcga
    15181 ggaggcgctt caggtcgtgc tggccgagcg cgaaggcgac gccgaggacc gccgaggcgg
    15241 cgccgaggag gaagagcgcg tcgccccagg aagcgggcgg gtcggggacg agcgaggtga
    15301 accggacgat cccgtagatc cccgtcttga tcaggacgcc cgacatgagg gccgagacgt
    15361 gcgccggcgc cgccgcgtgc gccggcggga gccagatgtg gagcggcagg aggcccgcct
    15421 tcaggccgaa gccgaggagg cagaggacga agagggccgt ccccgcgtcg ctccccgcga
    15481 cgcccgccgg gaggggcacg agctccgtcg atccggaggc ggagcggaag agggcgaaga
    15541 aaccgaagag gacgagcgtc gagacgtgcg tcgcgaagag gtagagacgc ccggcgtcgc
    15601 gcgcctcgcg cgactcccgg tcggccgtga cgaggaagaa ggccgcgagg gccatcactt
    15661 cccacgccgc gaggaagagg aggaggttgc gcgccacgag gacgagcgcg atcgccccgg
    15721 tcgacaggcc gaagaagaaa cgggtccgcg gcgcgtcgtc cgggtgcgcc tcgtcgccga
    15781 aggagcgagc gccgctcacg gccgcggccg cggagacgag gaagagcgga gcgaggaaga
    15841 gggccgagag ggcgtcgagc gagacgtgga aggccgcgcc cgggagcggc caggagcgct
    15901 gaatcgtctc gaccgtcccc gtgacggccg cgagcgccgc gcccgcgagg ccgaggaggg
    15961 agccggcgag ggagccggcg agggcgagcc ggcgtcccgt ctccgctcgc cgcaggagga
    16021 gccccggcac gccgccggcc gcgcagagga gagcgccgcc gaggacgagg aggaggctca
    16081 cctcggcagg acccacccgc ggagcgcgct ccaggccagg agcgcgacga gcgtgccgag
    16141 gacgtagacg aggtagatcg tcaggcgccc ttcctggagg gcgtggaggt cgtggaaacg
    16201 gcgcgcccag cgcgacgcga acggctcgag gagccggagg aggagcgggt cgcgcgcggg
    16261 tgccggcgcc gagacggcgg caccccgcgg gaagctcccg acgggggggc ggacctcgag
    16321 gaccggcgct gcggcggacg gcgtcagccg gccggcgatc cagtcgccga aggagctccc
    16381 cgtgtactgc atccgcccgg tcgggcgggc gtagccacag tcccaggtcg cgccgcgatc
    16441 gaaggaggcg cggcggaggc ggagcgcgag gagacccgcg acgagggcga ggaggccgag
    16501 gaacgcgacg ccgaggagcg cgatcgtccc gagtgggacg atcgcggcga gcggcggcac
    16561 gggatgggcg agctccccga cggagccggg cggcacggcc ggagcgaccc acgcgcggat
    16621 cgccgcgtcg acgcccggcg ccaggagcgg cgagaggagg ccggcgacgg cgacgagcgc
    16681 gccgaggacg aggatcggac cgagcatcgc ccgccccggc tcgtgcgcgt gcgcggcctc
    16741 ttcggagcgg gggctcccga ggaagacgac gccgaagagc ttcgtgaagg ccgcgagcgc
    16801 gaccgccccc gcgaccgcga gcgccggcgc ggcaaacgcg gcgaggagcg aggagtcggg
    16861 cccggcgaag gctcggaaga ggccgacgta gacgacgagc tccgagaaga acccggcgag
    16921 gggcgggaag gcgagcgccg ccgcgcagcc ggcggcgaac gcggccccgg tccgcggcat
    16981 cttccggagg agcccgccca gccggtcgac ctcgcgcgtc ccggccgcgt ggatcaccgc
    17041 ccccgcgccg aggaagagga gcggcttgaa gagggagtgg tagaggacgt ggaggagcgc
    17101 cccgccgagg ccgagcgcga cccactccgg ccggccctcc gagcgcccga ggagggcgag
    17161 gccgatcccc gtcgtcacga tgccgacgtt ctcgacggag gagtaggcga ggaggcgctt
    17221 gaggtctccc tgccccgcgg cgagcgccgc cccgaagagg gccgagccgg cgccgaggag
    17281 gaggagcagg ccgccccacc aggcgggcgg cgctccgtag agcgagacga cgcggaggag
    17341 gccgtagagg ccgaccttca gcatgacgcc cgacatcacc gccgagacgt ggctcggcgc
    17401 gccgccgtgc gcgccgggga gccagaagtg gaacgggaag agacccgcct tcccgccgaa
    17461 gccgacgacg gcgaggagga agacgacgct cgaggaggcc gtcacggcga ggccgccgtc
    17521 cggcgcctcc caggcgaaac cgcccgtcgt ccgcgcgagg agcgccgtca tcccgaagag
    17581 gccgagcgtc ccggcgtgcg tcgcgacgag gtagatccac cccgcctccc gcaccgaggg
    17641 ctgcctgtcc tcggtcgtcg cggcgaagaa ggcggcgagc gccatcacct cccaggcgac
    17701 gaggaagagg acgccgctcc gggcgaggag gacgaccgcc atcgacgcgg cgaggaggcc
    17761 gtagaagaag cggagcttgc gggcgtcgcg cctgtgctcc gcctcgggcc agtagccgag
    17821 gccgtagacg gccccgagcg ccgggacgag gaagaccggg acgaggaaga gggccgagag
    17881 ggcgtcggcc gagaggagga gcgggccgcc cgggagcggg ctcgggagct cgacgcggaa
    17941 gccgccgccg aggaagagga cgcgcgccga cgcggcgagc ccgaggagcg ctccggctcc
    18001 gacgaggagc gcggagagcc gctctccccc cgaccctccc ttccgggcga ggagcccggg
    18061 gagcccgctc agcgcgacga gggcgatcgc gccggcgagg agcaggccgt cgggactcgc
    18121 agcctccatc agaccgcccc ggcctccgcc cgccgtccgc cgccgcgcgc ctcgatctgc
    18181 cgcgcgagcg cgaggagctc gtcggccggc gcggcggccc tcacggccgc gcggaacgcc
    18241 tcgtcccgga ggagacaggc gagggcggcg aggaggtgga ggtgaccgtg gacggtcggc
    18301 gtcaccatcg tgacgaggac cgtcaccgga cggccgtccg acgcgccgaa gtcgaccggc
    18361 gcggcgagga aggagatgct gacgagcgcc tcgtcgacgt cgaggacgat cggggcccgc
    18421 acgtgcggga tcgcgatccc gtcgccgatc gccgaggagc cggcggtctc gcgcgcgagg
    18481 aggaaggcga ggagctcctc ggcctcgatc ccctcgggga gcggaaggcg cgagaccgcc
    18541 tcgcggagga cctcctgccg ggtcccgccg ggcagggcgt ggtggacccc gcccgccgcg
    18601 agcgccgcgg cgaaggaggg gagccgctcg ccgccggcct cggcggggag gaagacccgg
    18661 ggcgagacgg cgacgcggtg ctcggtcgcc cactccagga gggccgcgcg gttcaggagc
    18721 gtccgctcgg cgatccgctc ggcggggagg tcccgctcct cgatccagcg gtagatcgtc
    18781 gactcgtcga cgtcgaggag gtcggcggcg tccgcggtcg tcagcttcac ggcaccggct
    18841 cgccgcggac gttcttcttg acggagcgct tcccgcgttc cttcaccgag ccgagggagt
    18901 actcgatcgt cgtgtcgagc gcgagccagg aggtgaccgg cgagcctccc gtcctcgcgg
    18961 cggtgagcgc ccactccttc gcgctctccc ggacccagcg gaggatcatc ggctcgtacg
    19021 tcccgaccgg cacgacgcgc ttgaccgtcc cgaggcccga gacctcgacg agagcgagga
    19081 gacgcgactt caggcgcgtt ccctcgagct tgagcgggac cttcaccggc atcggcagga
    19141 agtcgacctc ctcgaccgag aggacgcccg gctccgcggg ggagaccgcg gccgggtacc
    19201 ggctcatcca cgtctctccc ggcatctcct tcggcagctc gggaagcggg tcttcctttc
    19261 cgaccgggac gagcgcgaac gcgtgccacg tcgcgtcctc gagctccacc tcgacgtcga
    19321 caccgtaggt cccccagcag cggaccgcca ctccgtcctt cgtcgccggc tggaacgtcc
    19381 agcgcggcgc ctgggcctgg acggcggcgg cgagcgcgcg gagcgggggc tcggggacga
    19441 ccgtctccga gacggccccc gcggcgtcca gcttcacgcc gacgatgagg ggaccggtcg
    19501 tctccaggtc ccggagcctc ggcggaggcc ctttcggggc cgccggagcg tcgagcgcct
    19561 gccagagcgg gttgacgaac gggacctcgg ccggcgcgag gcgagccggg tcgggaatcg
    19621 tctcgagcgg cgcctgcgcg gaaaggaccg gcgcgaggag gagggccggg agacagggaa
    19681 ggagcggccg ggcagcccgg cggccgacga aggccatctt tcggattgta gcgcccgcta
    19741 cttcgcggcc gcccagtcgg cgccgcgagc ggtctccacc gtcagcggaa cggcgagcga
    19801 cgcgacgtct tccatcgtct ccttcgcgag ggcggcgacc ggcccggcgt caccctcgtc
    19861 gcactccagg acgagctcgt cgtggacctg gaggagcatc cgcgccgacg ggaactccgc
    19921 cgtgaggcgg acctcgagat cgatcatcgc cttcttgatc aggtcggccg ccgagccctg
    19981 gaacggggcg ttcatcgcca tccgctccgc gttcgcgcga acggcgtggt tccggtcggc
    20041 gatgccgggg atcggccgga gccggccgaa gatcgtctcc gtcgacccgg tctcgcgggc
    20101 ctgcgcgagg atccggtcga ggcaggcgcg gatcgagggg aactgctgga agtaggcggc
    20161 gatgaacgcc ttcgcctccc cctgcgtcac gccgagctgc cccgcgaggg cgaacggccc
    20221 catgccgtag aggatcccga agttgatcgt cttggccccg cgccgctggt cgggcgagac
    20281 gaggtcgggg tggatgccga agatcctcgc cgcggttgcg cgatggatgt cgtcgccgcg
    20341 gcggaacgtc tcgatgagcg ccgcgtcgcc cgagaggtgg gcgaggaggc ggagctcgac
    20401 ctgcgagtag tcggcggaga cgaggagacg cccctgcggc gcgacgaagg ccttccggat
    20461 cgaccgtccg gcctccgtcc tcaccgggat gttctggagg ttcgggtcgt tcgaggagag
    20521 gcgccccgtc gccgcgaccg cctggtcgaa ccgggtgtgg atccgcccgt ccgcggcgag
    20581 gtgccgcggc agcgcgtcga cgtaggtccc cttcagcttc gagagctcgc gccactccag
    20641 aacgagaccg gggagcggtc cgaagccctt cagcgagagc tcctcgagga cctccgagcc
    20701 ggtcgcgtag ctcttcgtct tcgcggtctt cttcaggacc gggtagccca gcttctcgaa
    20761 gaggatccgc ccgagctgcg gcggggagcc gacgttgaac ggctcccccg cggcggcgtg
    20821 gatctccgcc tcgagccgcg cgaggcgggc gccgaggtcg acggagaggg tcgagagggc
    20881 gtcggggtcg acgaggatcc cggcccgctc catccgcgcg aggaccggga cgagcgggag
    20941 ctcgagctcc tcgtagaccc ggagcgaggc gggacgcgca ctcaggcgcg ggaagagggc
    21001 gtcggcgagc tcgagcggca ggcgcgcgcg cgggccgagg taggcgcggc ccgcctcggt
    21061 ggcgagctcg gccgcggtct cgaaggcgtc gctcccggcg acgtccttcg gcgacgggac
    21121 cgtctccggc gggcgaccga ggagggcgcg cgcgtcggaa tcgaggtcgt gggcgtggag
    21181 ccccggagag tcgacgtagg ccgcgagcat cgcgtcgaag acgcggcgcg gggccgggag
    21241 cgaaagcgcg gccgcggcga ggtggagcct tttcgactcg tgggcgacga gctccacgcc
    21301 ttcgtgcgcg aggagccgcg cgagaagggc gcgccccgcc tcggagcctt cgccccgaag
    21361 cggcgcggtc agcgcgccgc cgccgggaat cgcgacggcg agggcgatcg gctcgggcgg
    21421 gaagggacgc cccggcgcgc actcgacgtg aaggccgatg cggcccgcga cctccgaccg
    21481 cgcgaggaag gcgtcgagcg cggcgggggt gctctcccac gcgggaaggt cgccggaggc
    21541 ggagccggcc gtgacggccg ggccgccggc ttcttcagca tcgcgctcgc cggttccttc
    21601 gcccgccccg ctcacgggcg cggcggcgac ctctccggcc gtgtcgaaaa gcgacccgct
    21661 ccccgcggac ttcggcgcct ttgccggtgg cggcgcggtc gcggccgccg cctcgagcag
    21721 ctcccttcgg aggcgcgaga agccgagctc gccgaagaag gccgcgagcg cggccgctcc
    21781 ttccggcgac gccggaggga cggcgaacgt cccgaggagg ctctccacgt tgtcaccgcc
    21841 cggagtcggc gcgtcgcgcc ggacggtcgc gagctcgcgg gagcggaagg cttcggcttt
    21901 cccggcctcg agcgcttccc ggcgcttccc cttcagctcc ccgacgcgtg cgtagatctc
    21961 ctcgagggag ccgaacgtcg cgacgagctc cttcgccccc ttctcgccga tcccgcggac
    22021 gcccgggatg ttgtcggacg agtcgcccat caggccgagg acgtcggtga cgcgctccgg
    22081 cggcgctccg aagagctccc tcacgccctc cgagtcgagg agcttctcgt ggacgggatg
    22141 ccagacggcg acctcgcctt cgcggacgag ctggaagagg tccttgtcgg ccgagacgac
    22201 ctcgacggcg aggcccgccc gcgccccctt ctccgcgagc gtcccgatca ggtcgtccgc
    22261 ctcgaagccc ggctcctcga cgacgacgag gcccatcgcg cggcagagcg ccttcacgtc
    22321 ggcgacctgc gggacgaggt cgtcgggcat cgccgcgcgg gttgccttgt actccgggtc
    22381 catctcgtgc cggaacgtct tctcgggccg gtcgaagcag acggcgacgg cgtcgggctt
    22441 ccgctgggag aggagcttcc ggagcatcgt ggcgaagccg aacgtcgcgt tcgtcggccg
    22501 cccgtcgggg gcggtgagcg ggcggatcgc gtggaaggcg cggtagaggt agcccgaggc
    22561 gtcgaggagc gccagcgtct tcttttccgg catgcctcac ggagtctatc gggccggcgg
    22621 gaccgtaaga tggggcgagg cccgcccgtg tcgtccgtcc cgattccccc cgccccgccc
    22681 gtcacgaacg cccgggtcgc ctccgccctc ctcgccctgg ccgacgccga gccgcccggc
    22741 gcgcgcccgg cctccgagta ccgctcggcc gccgcgacgc tccgccagct gccggacgag
    22801 ctgcacggaa ggcggatcca cctcggcggc ctcccgcacg tcacgccccg ggccgccgac
    22861 gcgatcgcgg cgttcctctc ctgcggctcg gacgagtcgg tcgacgaccg cctcgccgcg
    22921 ctcctcgacc gcgacgaccc ggcgcgcgaa cggaagcgcg agttcctctc ccgcgccgac
    22981 gtcggccgga tcctcgccgc gccggagggg atctcgcccg ccgacctccg ggccgacgtc
    23041 cacctccaca cggaccgctc cgacgggcag atcccgctcg ccgacctcca ccgcgcgctc
    23101 gccgcccgcg gcgaccgcta cgcgctcctg accgaccacg cgcgtgattg cgccgtcgcc
    23161 ggcggcctcc acgcgggcga cttcgccgcg caggcgcgcg agctcgagcg gctgaacgcg
    23221 aggacggggg aggagttcgg gatgctcctc ggcgcggagg cgaacatcgc cgaggacggg
    23281 tcgatcgacg tcctgccggc gaccgtcccc tctctcgccg gcgtcgtcgc ctccgtccac
    23341 accgacctgc ggggcccgaa ggaccagacc gcccggctcg tccgtgcgat cgagcggccc
    23401 ggcgtggcga tcctgggcca cccgaagggg cgtctctaca acatgcggac cggcgtgcgg
    23461 gcgagctggg accgggtctt cgcggctgcg gcggagcgcg gggtcgcgat cgagatcaac
    23521 ggctgcccgg agcggcagga cctcccgccg tcgctcgccc ggaccgcgct cgccgccggc
    23581 tgcctcttcg ccctcgtcag cgacgcgcac gcccaccgcc acctcccctt ccagcgcgtc
    23641 tccctggcga tcgcacggct cgccgggatc ccggccgagc gggtcgtcaa cacctggccg
    23701 cgggagcgct tcgaggagtg gctcgaggag aggcgcctgg gttgaatcgt ccccgcacgc
    23761 ggcgctcctg atatcgttct ctcccacgca ttccggcaga ggaggcaaag atgggtcttt
    23821 tcggcaagtc gctcgacgaa aaagccgccg aggcggtgaa gagcctgcgc ggctcgatca
    23881 aggggctgaa gagcctcgat gcggtcgtcg agggaaagac cgtcacgctc accggcgagg
    23941 cggacgatct cgaggccaag ggcaaggcca tggccgagtt caacaagctc gtcgagaccg
    24001 agaacacgat caacaagatc gcgctcacga aggcgcccgt cgccgccgcg ccgacgccgg
    24061 ccgccggcgc cgtcgtgacc ggcggggccc ccgcggcgac cgcagccgag gccgccgcga
    24121 aggtccacgt cgtcgagaag ggcgacacgc tcggcgcgat cgcgaagaag tactacggca
    24181 aggccggcgc ctacatgaag atcttcgagg cgaacaaaga cgtcctgacg gaccccgaca
    24241 agatcaagcc ggggcagaag ctccgcatcc cggactgatc gccgggcttc ggccgggccg
    24301 cgggaatccg ccctccggcc cggccgttcg cacccggcct cgcgtcagtc gtagatcacg
    24361 gcgcccgggt cgaagcggtc gccggtgtcg ggcccgtgcc accgtcccgc gttcaggtcg
    24421 tagcgccagc cgaaggccgt ccagggaatc cggtaggtgc gggagaggaa gaggagcgtc
    24481 cggccccacc agctttcgtc ccggacaccc accgcccgga ggacctgctc gacgttcagg
    24541 ccgaggtcga tcccgacgtt tcgctgcctc acctcgacgg gcgagaagcg gtaccccttc
    24601 gacccgtagg tgagcgagac gagaaggaag cgcgccgggc cgggcgcgac gccgaggcgc
    24661 ggcaggagac cgtcgagctt caggtcgacg gactggaccc agcgcgagta gtcgccgccg
    24721 tacgccgtcg cccggcagca cgggtcgggg acccgggccc gcacgacgcc gaagcggaag
    24781 ccgatcgtgt cctcgaggcg gaggccgttc acccccgcgg ccgcggccgc gccgaggacg
    24841 ttcgccgcgg cgtcctccca ggaggcgccg taccgcgtga agccgtcgcc gatctcgatg
    24901 acgaggcccg cggccgagac gagggcgatg ccgaggagcc gcgccgtccg ctcgtccttg
    24961 ccgagggagc ggtagatcga ctccgcgccg tcctgcgcga tcgccgccat cacgaagtgg
    25021 gacgccttgt cggcgccgcc ggcgtagctg cgacgcccga gccctccctc ctcccgaaaa
    25081 tggaaggacg ccccttcctc ctctcgccac caggtgatcc acccggcgag cggcacggcg
    25141 gcgaggaccg ccgccgagac ccgcgccgtc cgggcgtcga agaccttctc cgccggcgct
    25201 accggcaccg cggcccccgg agccgcgccc accgcggatc cgggttccgc gaggggggag
    25261 acgggcggga gcccggtgag gggcgccgcg aggaggcgga ccccttcgag gcggaagccg
    25321 ggcttcgcct gtccggcggg cgccccgagg accggggccg acccgccgag gaagaggagc
    25381 aggaaagcca gccggcggcg gcggtggccc ttcagccggc gagctccttc gccttggcga
    25441 gggtccgcac tctctccatc gtctcggccg ccttcttcgc cgcctgctct gcgtcgctcc
    25501 ggaacccttc ggcgaggccg gcgtcggaga tgccggggag gttgatcctc acgttcgcga
    25561 gcgccccctc gagcgcggcc gagaggagga gggcggcgac ccaggcgtcg gaggcgcaat
    25621 tcgggttccc cttctcgagg acgacgggag ccttcgcgag cgcctccatc gagaagaagg
    25681 cggtctgcat cgggacggtc gtcgcgagga tcgtcgcctc gtcgatcgcc ttcttccgcg
    25741 ccgccttctc ctcgggcgtc tccttcggga gcttccgcgc cgcgaggatc gcgtcgtagg
    25801 cgcgggtgtc ctcgtcgacg aggccgagga gcgtgtcgcg gttcgcgtcc atcgcggagg
    25861 cgacgcgttc cagctcctcc cacgactcgc ggtatttctc cttgccgaga gtcaggcggc
    25921 agaccatgcc gaggagggcg gcgcccatcg cgcccgccgc ggccgaggcc gagccgccgc
    25981 cggg
//
LOCUS       DAOONL010000046        25523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1807_length_25523_cov_22.561567, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000046 DAOONL010000000
VERSION     DAOONL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25523
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_25523_cov_22.561567"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLP50_08770"
     CDS             <1..365
                     /locus_tag="PLP50_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51682.1"
                     /translation="LGYLLGNPTYLSVHADPRVGISPLEMAFLATYAASAILSNPRLF
                     LWRFLAAGWTNRLVFAGLFANQFVAPVVVAFLFSERRPRGPGLELFAMLGGVVATLTF
                     LLLQARLERRRAAAHATS"
     gene            complement(414..1178)
                     /gene="xth"
                     /locus_tag="PLP50_08775"
     CDS             complement(414..1178)
                     /gene="xth"
                     /locus_tag="PLP50_08775"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPA51683.1"
                     /translation="MKIATWNVNSVKARKERLFAFLSRHAPDVVCLQELKTEEKGFPF
                     EELRAAGYFAAVLGQKTYNGVAVLSREEPADVSTGFGDGGDESQARLVAATVGGLRVA
                     SAYIPNGKEVGSEKWPYKLEWLARLAAWVERQAGGPPLVLGGDFNIAPEERDVASPAA
                     WADSVLCHPEVRSAWERILAAGLVDSFRLHHPEGGLYSWWDYRMLAFPKNEGLRIDHV
                     LLAPALVPRCTGAFIDRDERKGKLPSDHAPVFAELS"
     gene            complement(1212..1928)
                     /locus_tag="PLP50_08780"
     CDS             complement(1212..1928)
                     /locus_tag="PLP50_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPA51684.1"
                     /translation="MPKTGQRRHPRYEVQNVHGSLLYRTTVKVRNVSVSGLSLESAER
                     LQLGRAYAIRLAGAGEAVDVQGTIRWCHLSSARPRPGGETRAVYEAGLAFDDVFTGNG
                     RSLLGFLEHHVVLSPQQRLTGRFRADTLLPAALEARDEFEVLRLSLSGMLVRTALEAS
                     VESRFALELRLRTGTVPLEGRVAFVQREEAEKGSVAAQVGIEFVGGTDEARAAVARFI
                     SAELERRPGDPPEGSVPAAS"
     gene            2008..2808
                     /locus_tag="PLP50_08785"
     CDS             2008..2808
                     /locus_tag="PLP50_08785"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPA51685.1"
                     /translation="MPNAAKAAAPDPTAVDAAARILRRARSVLFVTGAGISADSGLPT
                     YRGVGGLYESDATEDGLPIEVLLSGETFRRSPATTWRYLGAIERACRGARPNRAHQAI
                     AALENALPRVVVLTQNIDGLHRAAGSRDVIEIHGDVHRLACTRCTFRTRVPDYEGLEM
                     PPRCPECAAPVRPEVVLFGEMLPTEAVSRLEEELRRGFDAVVSIGTTSVFPYVAAPVH
                     MARAWGAGTVEVNPGETEVSGIVDVRLRCGAAAAMDALVSAANRSPVG"
     gene            2841..4478
                     /locus_tag="PLP50_08790"
     CDS             2841..4478
                     /locus_tag="PLP50_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA51686.1"
                     /translation="MSLRLRVVSADGRRFEHDVAGDALVIGRSSRADLALADRAMSRE
                     HARFRRDEAGWWIEDLGSHNGTRLNEVPLDAARRIHDGDTISVGGSVLVAEIRGAGDT
                     SSGDSVIYRSARELLEAVSGSTDVSGIAGAGKRAAERLHMLNGVNQALASSISLEELL
                     ELILDRAFEHLRPQEAAIFLRDASGVDICAARRTTPGREALPMRSKSLFHEVIDKGMA
                     AHVVDSAADARFAESRSLILSGLRSFLAAPLLDSQGALGLIVVGAALGVRSFKSEDLE
                     LLVSLASVAALRIRNVRLVAEAMERQRLEQEVRLARQIQVALLPATLPQLPGWELHGG
                     TLPSRGVSGDFYKLLLRGDGTSCALFVADVSGKGIAASLLTASLEALSALPLEGSDPP
                     ARICEQVGALLHRRTPAEKYATAFLGVFDPPSGRLIWTNAGHNPALLLRAGGEAEWLR
                     SNGVPLGLIPGAKYGEGELVMEPGDVLLVYTDGITEAADPDDEEFGEERLLEAARRGG
                     TLPLREMATVLERDLDAFARGVPFHDDRTLVLIRRSA"
     gene            4490..5263
                     /locus_tag="PLP50_08795"
     CDS             4490..5263
                     /locus_tag="PLP50_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPA51687.1"
                     /translation="MYFRLVRLKVRPESFWAFRDYYEGRILPGLASAGGCLYAALLRP
                     VRPAEEAEAACDSLTLWESEERADAYVDSGRYDELLDGADPFLAGPTVWSSDLTRLEV
                     GDRPPLPDPKVETGSVEAGQEADGGLTLPALFLRVVDHRIDPGSLDALQRTWEEDVVP
                     GLLSTPGCLAAYLVEGLRGREQALSLTFWSDEASAVRYELSGRFDELAGKLAPYLSGL
                     YRWRLGLETDGAARNLRGSDLAVSAFHVVAGRRLGEPTG"
     gene            complement(5277..5867)
                     /locus_tag="PLP50_08800"
     CDS             complement(5277..5867)
                     /locus_tag="PLP50_08800"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPA51688.1"
                     /translation="MEDRVGLADLARSLERHQGAVETGVPADRKAAVAAILREHSGGA
                     ELLFIRRAEHPRDPWSGQMGLPGGRVEPADASPLDAALRETLEEIGLDLAALGRPLGR
                     LSEVRTHLPLGSVPHSVVPFAFSVDGDPRLAPNEEVQEALWVPLSFLADGRNRNSFTW
                     VRKGLPLPMPCIRFEGRVIWGLTLRIVDELVSVAAG"
     gene            6016..8643
                     /locus_tag="PLP50_08805"
     CDS             6016..8643
                     /locus_tag="PLP50_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA51689.1"
                     /translation="MLERSARLGPYEILDPLGAGGMGEVYRARDGRLGREVAIKVLRG
                     AFARDGDRIARFEKEARALAALSHPNVLAIHDFGRSGDVVYAVTELLDGETLRERLRH
                     GRPSWRKSAEIAAAAADGLAAAHARHLVHRDLKPENVFLTADGRVKVLDFGLAKVVEP
                     VLPEASTLTSPASTAEGRLLGTVAYMSPEQARGLGVDSRSDLFSLGTVLYEMLSGRRP
                     FERATAADTISAILHEDPDPLDAIAAIPPALAAIVARCLEKEPEERFHSAHDLAIALR
                     SLSAENLSGARPVRSRRRPARARPALLAAAAVAVLAAAALLVADRARERPGRETVQVV
                     ARPVTSMPGWEIEPAVSPDGSLVAFASNAAGSADVWLVESSGGEPRQLTDVGIPGDDA
                     PEDRSPAWLPDGRSLLFSSDRNGSRSLFRVSFLGGSPSLVVEDAAEPALSDDGTVLAF
                     TRTVGNGRYRRIWVAPLAAPDDARAITTDEDGLWDHARPALSPDGRRLCYADFRNLWI
                     VDLPGGRPRPLTADDAFDAEPRFSPDGRHVYFTSRRGDVVAVWAVPADGGAATRITGE
                     AASETHPSFSRDGSVLVTATRVRDSDVAVLDRVAKTVARISSTLSEETPVLEPDGRAV
                     YFSSNRTGRSNLWKQPLHAGRSVGPANRLTSFEAGGPATPSISRDGRFLAFFRALSGN
                     RDIWILPTAGGIARPLVGGPDREFHPALSPDARQLVFVSSRGGVEHLYTVPLRDGAAE
                     GEPRPITFGASADAFPVLSPDGSRIAFLRDGEIHLIDSEGRGEPERLTHDARPNHFAW
                     DAGGDALVVAGRFSGRRLVLRRVRLDGVVEPIEPEVELGGELSSGYLSVSADGRFLAV
                     QVGRLEADVWVRAPFTGGP"
     gene            8714..9139
                     /locus_tag="PLP50_08810"
     CDS             8714..9139
                     /locus_tag="PLP50_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51690.1"
                     /translation="MPTTLVHRLELMRLLHRFKGLVEWELEKLAALDTRTISLELEPP
                     AFAVRKPDRKPPPIATGTEPAAEAPAVSETPSIPGADAPDGSLADLSRPAGAPSHPLQ
                     KTKDPTNLESCCRSAGLPTGDYAELVDAATPYVRALAEE"
     gene            9136..10359
                     /locus_tag="PLP50_08815"
     CDS             9136..10359
                     /locus_tag="PLP50_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA51691.1"
                     /translation="MTRIDDRAVRPLVPGPRAVTLLLTTRCNLSCAYCPQRRGAPREM
                     DAAALEAAVRLVATSGHARPKLVLFGGEPLLAKALVRRVLALLAASARPGLAPDVHVV
                     TNGLLLDAETAASLDEAGVTIELSCDGFDAAQERRGAGTGADLRSVFLLLARSRPRLL
                     AERLVARVTVDSGNVGALGRSVAELRAAGIREIRAAPVQTPDPGWSEASADLLDRALS
                     EIAALPPNGNGDSTFDPLRPSAGPRNPFGVAACSLGEADVLFVDVDGRIAPCGAFAPS
                     VLPSLPPLAEELRQRLVGLRVDDPALDTRLALRAAGRQSLPALVASAARRSPRGPCAS
                     CEALDECAVCPAAIAGAPEQDPDLVPAIWCDWNRRTARHRRASLRSAVDDQRAGSSSS
                     APETSAVTAVRIAGA"
     gene            complement(10287..11600)
                     /locus_tag="PLP50_08820"
     CDS             complement(10287..11600)
                     /locus_tag="PLP50_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPA51692.1"
                     /translation="MRPAAAALAAGLAVPALAAPPAPRLQAVLAPLAGETEPLGELYR
                     ELHATPELSMQETATAAKMAGRLRALGYEVSEGVGGTGIVGVLRNGDGPVVMLRTDLD
                     ALPVEEKTGVSFASRATGKADDGTPTPVMHACGHDLHMTAWTGAATLLARAKGSWRGT
                     LVMVGQPAEEKGAGAAKMLKDGLFSRFPRPDFALALHVTPELPAGSVGVTPGAAFASV
                     DSVDLTIHGKGGHGAMPHRTVDPVLIAARTVVALQALVSREKDPFEPAVVSVGSFQAG
                     AKHNVIPPEARLKITVRAYREEVRQRLLEGIRRVATGESIAGNAPKAPDFSIVESTPG
                     VVNDASLTERLRTAFTAALGAANVASFPPASVSEDFSYFGREGVPASLFWLGVAEPAA
                     LAKAKADGTSLPPLHSAEFAPAYAPAIRTAVTALVSGALELLPAR"
     gene            11646..13157
                     /locus_tag="PLP50_08825"
     CDS             11646..13157
                     /locus_tag="PLP50_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPA51693.1"
                     /translation="MSAETPGWVRDAVFYQVFPDRFARSAQVAKPANLEAWDSPPTAH
                     GFKGGDLLGLAEKLDDLSALGVTALYLNPVFASAANHRYHTWDYHQVDPILGGNEALR
                     ELLDAAHARGMRVVLDGVFNHVGRGFFAFHSVLENGADSPYLDWFHLDLERLQDGRPL
                     GAYAEPKLLRRSKKPKPAFEELGYSAWWDCPALPKLDTRTPAVREYLWDVAERWIRFG
                     ADGWRLDVPSEIDDDEFWRELRRRVKRANPEAYLVGEIWHDARRWLAGDQFDATMNYP
                     LAKAALGFFLPELETDALRSVGGYRDVVELDAAAFAAEVERVLGLYDPAVRDAQLNLI
                     GSHDTPRFLTLAGGELAALEMAFLFLFTMPGAPCIYYGDEIGLEGGPDPDCRRTYPWD
                     EGSWDHVLREQVRRLAALRRAHPALRAGAYRTILAEDAIVSYVREEEGGDAVVVAFNV
                     SREERRVDLPVGALPERLLDAATGDDLVVTDGELRGIDLPGRSGRAFVTPPAG"
     gene            13654..15912
                     /gene="ccoN"
                     /locus_tag="PLP50_08830"
     CDS             13654..15912
                     /gene="ccoN"
                     /locus_tag="PLP50_08830"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HPA51694.1"
                     /translation="MSDTVRYDDRTPRLFFLAAIFWAVVGMLVGVLAAAMLFLPELNL
                     APYFTFGRLRPLHTNAVIFAFVGNMIFGGTYHSMQRLLKARLFSDALSKIHFWGWQLL
                     IAAGAVALVAGVTQAKEYAELPWVLDVVITFLWVVFAVNFFGTIARRREEHLYVAIWF
                     YIATIVAVAILHIGNSMAIPYSWLGSYSAYAGVKDALMQWWYGHNAVAFFLTTPYLGL
                     MYYYLPKAAERPVFSYKLSIMHFWSLVFVYIWAGPHHLHYTALPEWASTLGMLFSLIL
                     WMPSWGGMVNGLLTLRGAWNKLRDDPVLKFLVVAVTYYGMSTFEGPMMSIKSVNAVSH
                     YTDWTIGHVHAGTLGWNSFLSFGILYWIVPRLWKTELFSKKLATAHFWIGTIGLILYQ
                     VSMWVAGITQWAMWRAFEPDGRLVYPDFIETVLRIVPMYWVRLVGALLFFAGLLVMLY
                     NLVRTMRSAPAGFDEEPEVKAPPLVVDRSAPGATTAPGTWDHAFYRFQHVIRHGVHRA
                     IEGRVVLLTVMTALALSVGSLVEAIPLFFDESNVKTIASVKPYTPLELLGRDIYVREG
                     CYNCHSQMVRPFRHETERYGEYSKAGEFVYDHPFQWGSKRTGPDLHRVGAKYPSLWHV
                     RHMDQPRSTTPGSVMPRYPHLLKDPIDDSLVEAKMRAMRTVGVPYSDGEIATAKAAIA
                     AQAQAIASEVEAQQGPPGLADREITALTAYLQRLGTDIRWRRPEVQPPLALPAAPAAA
                     SVPAAAPVAAAR"
     gene            15918..16121
                     /locus_tag="PLP50_08835"
     CDS             15918..16121
                     /locus_tag="PLP50_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51695.1"
                     /translation="MLQELARESGAQAWAIASMLFFLGVWLVLAVRVVRARPDALAAR
                     ARLPLADDEAASPALAAEPGRGA"
     gene            16134..16721
                     /locus_tag="PLP50_08840"
     CDS             16134..16721
                     /locus_tag="PLP50_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPA51696.1"
                     /translation="MAEYEDKVLHDIDGIKEYDNPLPGWLMAIWWGAIAFSVLYLAFY
                     ALRFGERSMDSEYRAETERALASVQAHFDANPLVAPTSAELLAGAADPVVLDRGAGRF
                     AKSCASCHGEKAQGLIGPNLTDDRWIHGGSVEQVFQSIVKGWPAKGMPPWGRAIPPEE
                     ISALVSYVRSVQGSSPANAKPAEGDPFPPETIPGR"
     gene            16723..18195
                     /gene="ccoG"
                     /locus_tag="PLP50_08845"
     CDS             16723..18195
                     /gene="ccoG"
                     /locus_tag="PLP50_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HPA51697.1"
                     /translation="MAGPAAAKEERARAERPAGRERVFDVAPEEELLYSMSADGSRKF
                     IRPVLHRGRYWKIRRAMAWGLFVLFVALPLVPLGGHPAVHFDLGKRLFHVLGATFHPT
                     DNVLLLLFGFGVIVTVFFVGSTFGRMWCGYACPQTVYLEFFFRPVELLLEGAPLAQRK
                     LNAAPLSPRKAGIKTAKWAIWALLALLLASTFVAYFVSWKGLFAGLAAAPGEMTGALG
                     TIAFLTALILFDFGWFRDQMCTVACPYGRLQSVMADPDTILVAYDAKRGDPKVKVKDR
                     VAGVAAGDCIDCRSCVNACPTGTDIRRGLQSECIGTAQCIDACDEVMRGQKKPIGLIK
                     YTSEREEAGGTRRLVRPRNLIYLALLTLAWGSLAFLLLTRADALVEIVRGGREPYRVL
                     VTGEVANQQRLRVTNQLDVPQRFSVEVLSPGGARFVLAGGPVDVAPEKVVTVNVVTTV
                     PAEVFRDGQATARYVVTSDRGFRKEIEFLLLGPWGKGAAK"
     gene            18192..18725
                     /locus_tag="PLP50_08850"
     CDS             18192..18725
                     /locus_tag="PLP50_08850"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HPA51698.1"
                     /translation="MKLRKFLSEYRWPLYLTGLLTMSILAYAILVWVATRPTAPRPIA
                     GFYEASQEWDADEAVVAASRALGWTVTYELPAGVPHVAGTPRPVDVQVTDRDGQGVTG
                     LSGRLFALRAAEARRGPPGTLVELPHRPGSYRALLRVDEPGAWDFRLDARRDGQRFVH
                     SARVAVEKDAPEGGSAR"
     gene            18722..21250
                     /locus_tag="PLP50_08855"
     CDS             18722..21250
                     /locus_tag="PLP50_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HPA51699.1"
                     /translation="MKGPRERSHLHAAPVACAHCGLAVPEGMLRPGEEKQFCCNGCRQ
                     VHDLIRDWGYEKFYGLVEQQGGVLEPARPTGRAFEDLDDPRALEGSSDEEPGGRCRTR
                     LYLEGVHCAACVWLVEKLPEALDGVDSVRLNLASAVAEVTWRPGTVKLSAIARALDRL
                     GYSPHVHRAGAARDARRAEDRATLVKLGVAAAGAMNLMFLHGALYAGEASGMASPYET
                     FFRWVSLGIALPVLAFSARPFFQTAYAGLKSRFVHIDLPVAIGIAVTFAASAANVVRG
                     SGPVYFDSLAMLVAALLGARQVQRAAQRAALERADSLRGVAFLEFARRLEGDGPLAPA
                     VEVPVAALEPGDRVEVRSGELVPADGVVLFGRSTLDGSVLTGESAPVPVAEGGAVWAG
                     VTNLGARLVVKVEAAGEKTRVGALLAVVEDALARRPAVLRRTDLLARRFVQALLLLAA
                     AALLLALPLGTEAAVGRVVALLVVACPCALGLSVPLAVSVGLMRAARAGIFVKDPDAL
                     DRLRRVGTVLLDKTGTLTEGKATFARYLGDEAALELATTLEAESSHAVAHALRASLGR
                     RVNVVREVTEVREVAGRGISGVVDGRRLAVGNRAHVEALAAPAPGELAAHAEALVADG
                     FSPLWVAVDGRVVGVGGIGDPLRADARATVDALRARGVRVRILSGDDPRVVASVAARL
                     GIAPADALGGRTPEEKRDVVASLVGAREGEERRAPAVVMVGDGVNDAAALALADVGVA
                     VLGGKGASLVAADVVLTREGVAPLLDLLRGSRRVFGVIRRNLAFSLLYNAAGAALALL
                     GLVGPLLAAVLMPVSSLSVVLSSALSRPFPAAARRAARTHGAEA"
     gene            21251..21493
                     /gene="ccoS"
                     /locus_tag="PLP50_08860"
     CDS             21251..21493
                     /gene="ccoS"
                     /locus_tag="PLP50_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HPA51700.1"
                     /translation="METIFVLLPLALLIAGIALALFIWAARSGQFDDLETPAVRVLFD
                     DEPAPAAPRPALGEPAASPAPNPPETTSDAPTRRTE"
     gene            21490..22113
                     /locus_tag="PLP50_08865"
     CDS             21490..22113
                     /locus_tag="PLP50_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPA51701.1"
                     /translation="MSRPRTPVRILILLLGMVAAGSLAYALSNVRKGYEPRQPILFRH
                     SRMAGPAVMQTNEKGEAVNVGGYDIPCLTCHTMPYKGRHATVPSTAVCMNCHTAVGLN
                     REWVLKMKDYWDRGEPIPWVKVHDLPDFVYFDHSAHLNAKDEKGMPKLPLKDAEGRPW
                     QICANCHGRVEETDIVSVTNAFNMQWCLDCHRKPEMKASTDCVACHR"
     gene            22126..25263
                     /locus_tag="PLP50_08870"
     CDS             22126..25263
                     /locus_tag="PLP50_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA51702.1"
                     /translation="MRPNETSPRPEAGVDRREFLRLGSALAAAGALASAGCQPPQEAT
                     IPFHDMPESLAEGIGRARFFHTVVDGTPVLVRTREGRPILVAPSPTDASGRGLTLRHQ
                     AALMDLYDPDRARGPLSVRRGQGPTVASSWKTVGADVVSRLAKAGSGAVLLTGPVASP
                     ALSEAIAALSAATGLRHVAWSPIGGDAAAAAHRAAFGDERLPRPLLGKADLILGLGAE
                     ILDRPADGLERDFAMRRSPDAPEAKMSRFVQLEGRLSLTGANADERVRVRDSQLASVA
                     AALAHELVVTRKAGPLAGSPEVAAALAPFAPEAVAKRASLDPALLSRLAGELLAARGK
                     AIVLAGGAASGSASGEALELVALLLNLTLDAYSAGLFDEAAAERPAAGGAAALAALSD
                     EMRAGKVSLLLVAGANPVYDAPPAVRFAEAIAKVPFVVSLNDRLDETSLLADLFAPAS
                     HPFECWSDAALPKGLLAIQQPVIQPLYDTRGLLDLLVEWGAAAGAGGELAAAVAAATA
                     ALAKAPLPASAAPAPSPSFAWHFLRAAWAPRLGAAPGSPELEKAWNELLRAGAFQGPA
                     SAAPPARTLALAALALLSSIPEAPAGLELQLYPHLALHDGRAGNNAWLQEFPDPITRI
                     SWGGALSIAPRRFDEMGLANGDLVEVDAGHAKLVAPAYRHAGMHADEVALPLGLGRAS
                     CGPIGAGVGPNAFALGLLTGGRLLSAGLPVKIRKFGGREELAFAQGSDVIDRDRRPLV
                     PVTTLSAYEKDPKSGTEQQHGGPSAWPEHDYPNARWAMAIDLSKCNGCGKCVLGCQAE
                     NNIPVVGRRGIVDGREMSWMRIDRYYDAPKKEGRWDAEVWDGPLPVVEEPKVLFEPML
                     CQHCENAPCETVCPFVATMHSEDGLNQQIYNRCVGTRYCANNCPFKVRRFNFFEYGKA
                     QESALFRALEPRIKRNAELNTRSPMQMKNNPEVTVRSRGVMEKCSFCVQRIREARAAA
                     TRDGKEKTHFPDGAVVPACMEACPTGAITFGDVNAPGSKVAALSAHPRAMRLLDALGV
                     KPSISYLTKVRNDKA"
     gene            25250..>25523
                     /locus_tag="PLP50_08875"
     CDS             25250..>25523
                     /locus_tag="PLP50_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51703.1"
                     /translation="MTRPEASPYATVMDDSQLTGRPIVLAGAGPYGGDLEVPEPLVTG
                     AKKWSDVDQDICMHAERFPTKRWWAAFAVALTLLGVLVVSLVTLLYA"
BASE COUNT         3275 a         9945 c         8704 g         3599 t
ORIGIN      
        1 tcctcggcta cctcctcggg aacccgacct acctgagcgt ccacgccgac ccgcgcgtcg
       61 ggatctcgcc gctcgagatg gccttcctcg cgacctacgc cgcgagcgcg atcctctcga
      121 acccgcggct cttcctctgg aggttcctcg ccgccggctg gacgaaccgc ctcgtcttcg
      181 ccgggctctt cgcgaatcag ttcgtagcgc ccgtcgtcgt cgccttcctc ttctccgagc
      241 ggcgcccgcg cggccccggc ctcgagctct tcgcgatgct cggcggcgtc gtcgcgacgc
      301 tcacgttcct cctcctgcag gcgcggctcg agcgccgccg cgcggccgcg cacgcgacga
      361 gctgagcccc gttctcctgt ccgacgacgc cggacgcggc gggcccggcc gcctcaggag
      421 agctcggcga agaccggtgc gtggtcggaa gggagcttcc ccttccgttc gtcgcggtcg
      481 atgaatgccc cggtgcaacg cgggacgagc gcgggcgcga ggaggacgtg gtcgatgcgg
      541 aggccctcgt tcttcgggaa cgcgagcatc cggtagtccc accaggagta gagcccgccc
      601 tcggggtggt ggaggcggaa cgagtcgacg agcccggccg cgaggatcct ctcccacgcg
      661 gagcggacct cggggtggca gaggacggaa tcggcccacg cggcggggct cgcgacgtcg
      721 cgctcctcgg gggcgatgtt gaagtcaccg cccagtacga ggggtgggcc tcccgcctgg
      781 cgctcgaccc acgccgcgag gcgcgcgagc cactcgagct tgtagggcca cttctccgag
      841 ccgacctcct tgccgttcgg gatgtaggcc gaggcgacgc gcaggccgcc gacggtggcc
      901 gccacgaggc gcgcctggct ctcgtcgccg ccgtcgccga agccggtcga gacgtccgcc
      961 ggctcctcgc gggagaggac ggccaccccg ttgtacgtct tctgcccgag gaccgcggcg
     1021 aagtagcccg cggcgcggag ctcctcgaac gggaagccct tctcctccgt cttcagctcc
     1081 tgcaggcaga cgacgtcggg ggcgtgcctc gagaggaacg cgaagaggcg ctccttccgg
     1141 gccttcaccg agttcacgtt ccaggtggcg atcttcatcg ggcctcccgc gccgccgggc
     1201 gcggcgcgat tctaggaggc cgcggggacg gacccttccg gcgggtcgcc cggccggcgc
     1261 tcgagctcgg cggagatgaa ccgggcgact gcggcgcgcg cctcgtcggt gccgccgacg
     1321 aactcgattc cgacctgcgc cgccaccgag cccttctccg cttcctcccg ctgcacgaac
     1381 gcgactcgcc cctcgagggg gaccgtcccc gtccggaggc ggagctccag ggcgaatcgg
     1441 ctctcgacgg acgcctcgag ggcggtccgc acgagcatcc cggagaggct cagccgcagg
     1501 acctcgaact cgtcccgcgc ctcgagcgcc gccgggagga gcgtgtcggc ccggaagcgc
     1561 cctgtcaggc gctgctgcgg ggagaggacg acgtggtgct cgaggaagcc gaggagggag
     1621 cggccgttcc cggtgaagac gtcgtcgaac gcgaggcccg cctcgtagac cgcccgcgtc
     1681 tcgccgccgg ggcgcgggcg cgcgctcgag aggtggcacc agcggatcgt cccctggacg
     1741 tcgaccgcct cgcccgcccc ggcgagccgg atcgcgtagg cgcggcccag ctgaagccgc
     1801 tcggccgact cgagggagag gcccgacacg ctcacgttgc gcactttcac cgtcgtcctg
     1861 tagaggagcg agccgtggac gttctggacc tcgtaccgcg gatggcggcg ctggccggtc
     1921 ttcggcatgc tcgtctcgtc ctccggtgag cgatcgcggg cgtcgcgatt atgacgccgt
     1981 tgcgcccgga ccgtacactg ggccgtcgtg ccgaacgccg cgaaggccgc cgcgccggac
     2041 cccaccgccg tcgacgccgc cgcccggatc cttcgccggg cgcgctccgt cctcttcgtc
     2101 accggggccg gaatctccgc cgactcgggc ctcccgacct accgcggcgt cggcgggctc
     2161 tacgagtcgg acgcgacgga ggacgggctc ccgatcgagg tgctcctctc cggcgagacg
     2221 ttccggcgaa gtccggcgac gacctggagg tacctcggcg ccatcgagcg cgcctgccgc
     2281 ggcgcgcgac cgaaccgggc ccaccaggcg atcgccgcgc tcgagaacgc tctcccgcgc
     2341 gtcgtcgtcc tcacccagaa catcgacggc ctccaccggg cggccggctc gcgggacgtc
     2401 atcgagatcc acggcgacgt ccaccggctc gcctgcaccc gctgcacctt ccgcacgcgc
     2461 gtcccggact acgagggcct cgagatgcct ccccgctgcc ccgagtgcgc cgcgcccgtc
     2521 cggccggagg tcgtcctctt cggcgagatg ctccctaccg aggcggtctc ccgcctcgag
     2581 gaggagcttc gacgcggctt cgacgccgtc gtctcgatcg ggacgacgag cgtcttcccc
     2641 tacgtcgccg cgcccgtcca catggcgcgc gcgtggggcg cggggacggt cgaggtgaac
     2701 ccgggcgaga ccgaggtctc cggaatcgtc gacgtccgtc ttcgctgcgg cgcggcggcc
     2761 gcgatggacg cgctcgtctc cgccgcgaac cggtcgcccg tcggctagac tgcctcggaa
     2821 cggacaacac gggggaacgc atgtcgctcc gactgagagt cgtctcggcc gacggtcgcc
     2881 gcttcgaaca cgacgtggcg ggcgacgccc tcgtgatcgg ccgatcctct cgggccgatc
     2941 tcgccctggc ggaccgcgcc atgtcccgcg agcacgcccg gttccgtcgc gacgaggcgg
     3001 gctggtggat cgaggacctc ggctcgcaca acgggacccg gctgaacgag gttcctctcg
     3061 acgccgcgcg ccggatccac gacggtgaca cgatctccgt cggagggagc gtcctcgtcg
     3121 ccgagatccg cggcgccggc gacaccagca gcggggactc cgtcatctac cgctccgccc
     3181 gcgagctcct cgaagcggtc tcgggctcca cggacgtctc cgggatcgcg ggcgccggga
     3241 agagggcggc cgagcgcctc cacatgctga acggcgtgaa ccaggcgctc gcgagctcca
     3301 tctcgctcga ggagctcctc gagctgatcc tcgaccgcgc tttcgagcac ctccggccgc
     3361 aggaggcggc gatttttctg cgcgacgcct cgggggtcga catctgcgct gcccgtcgga
     3421 cgacacctgg gcgcgaggcg ctcccgatgc ggtcgaagag cctcttccac gaggtgatcg
     3481 acaaggggat ggccgcccac gtcgtcgatt ccgcggccga cgcccgcttc gccgagagcc
     3541 gtagcctgat tctctccggc ctccggagct tcctcgccgc gcctctcctc gactcccagg
     3601 gagcgctcgg gttgatcgtc gtcggcgccg ccctcggcgt gcggagcttc aagagcgagg
     3661 acctcgagct cctcgtctcg ctcgcctcgg tggcggcact ccgcatccgg aacgtccgcc
     3721 tcgtggccga ggcgatggag cggcagcgcc tcgagcagga ggtccgcctc gcgcggcaga
     3781 tccaggtcgc cctcctcccg gcgacgcttc cgcagctccc cgggtgggag ctccacggag
     3841 ggacgcttcc ctctcgcggc gtctccggcg acttctacaa gctcctcctc cgcggcgacg
     3901 gcacgtcctg cgcgctcttc gtcgcggacg tctcgggcaa ggggatcgct gcttccctgc
     3961 tcaccgcgtc gctcgaagcg ctctccgcgc tccccctcga gggatcggac ccgccggctc
     4021 ggatctgcga gcaggtcggg gcgcttctcc accgccggac gccggccgag aagtacgcca
     4081 cggccttcct cggcgtcttc gaccctccct ccggacggct catctggacg aacgccgggc
     4141 acaacccagc gctcctcctg cgcgccggcg gggaggcgga atggctccgg tcgaacggcg
     4201 tcccgctcgg gctcatcccg ggggcgaaat acggcgaggg cgagctcgtg atggagccgg
     4261 gagacgtcct cctcgtctac accgacggga tcacggaggc cgccgatccg gacgacgagg
     4321 agttcggtga ggagcggctc ctcgaagcgg cccgtcgagg tggcacgctc ccgcttcgcg
     4381 agatggcgac cgtcctcgag cgtgacctcg acgcgttcgc gcgcggcgtc ccgttccacg
     4441 acgaccggac gctcgttctc atccgcaggt cggcgtgagg acccggcccg tgtacttccg
     4501 cctcgttcgc ctgaaggtcc ggcccgagag cttctgggcg ttccgcgact actacgaggg
     4561 ccgcatcctc cccggcctcg cgtcggccgg gggatgcctc tacgccgcgc ttctccgccc
     4621 ggtccggccg gccgaggaag cggaggcggc gtgcgactcg ctcacgctct gggagagcga
     4681 ggagcgggcc gacgcctacg tcgattccgg ccgctacgac gagctcctcg acggcgccga
     4741 cccgttcctc gcaggcccga cggtctggtc gtcggacctc acccggctcg aggtcggcga
     4801 ccgccctccc ctgcctgacc cgaaggtcga gaccggatcg gtcgaggccg gacaggaggc
     4861 cgacggcggc ctcacgctcc ccgcgctctt ccttcgcgtc gtcgaccatc ggatcgatcc
     4921 cggcagcctg gacgcgcttc agcgaacctg ggaggaggac gtcgtccccg gcctcctctc
     4981 gactcccggc tgtctcgccg cgtacctcgt cgaagggctc cggggccgcg agcaggcgct
     5041 ctcgctcacg ttctggtccg acgaggcgag cgccgtgcga tacgagctga gcggccggtt
     5101 cgacgagctc gccgggaagc tcgcccccta tctctccggc ctctaccgct ggcgcctcgg
     5161 gctcgaaacg gacggcgcgg cgcggaacct gcgcggaagc gacctggcgg tctccgcgtt
     5221 ccacgtcgtc gccggccgga ggctcggcga accgacgggc tgagcgcgtc cgcgcgctac
     5281 cctgccgcga cggagacgag ctcgtcgacg atccggagcg tgagccccca gatcacccgt
     5341 ccctcgaagc ggatgcaggg catcgggagc gggagcccct tccgcaccca ggtgaacgag
     5401 ttccggttcc ttccgtccgc gaggaacgag agggggaccc aaagagcctc ctggacctcc
     5461 tcgttcggcg cgagacgagg gtctccgtcg accgagaagg cgaaaggcac gacgctgtgg
     5521 gggacggagc cgagcggaag gtgtgtccgg acctcggaga gtcgcccgag cggacgtccg
     5581 agcgcggcga gatcgaggcc gatctcctcc agcgtctccc ggagggccgc gtcgagcggg
     5641 gaggcatcgg ccggctcgac gcgcccaccc gggagcccca tctggccgga ccacgggtcg
     5701 cgcgggtgct cggcccgacg gatgaagagg agctccgccc cgcccgaatg ctcccgaagg
     5761 atcgcggcga ccgccgcctt ccgatccgcc gggacgcccg tctcgacggc gccctggtgc
     5821 cgctcgaggg aacgggcgag gtcggcgaga ccgacgcggt cttccacgtc cggattctgg
     5881 catccgtgtc gcaggcgccg gccggccgcc cggaagcgcg cgagctcccg agcttcccct
     5941 cgcctttctc gttgacgcgg ccgtccggga agaccacagt tctcctggaa cagcccggaa
     6001 cgaacggact ccgacatgct cgaaaggagc gcgaggctcg gtccgtacga gatcctcgac
     6061 ccgctcgggg ccggggggat gggcgaggtc taccgggcgc gcgacgggcg gctcggacgc
     6121 gaggtcgcga tcaaggtcct tcgcggcgcc ttcgcgcggg acggcgaccg gatcgcccga
     6181 ttcgagaagg aagcccgtgc gctcgcggcg ctctcgcatc cgaacgtcct cgcgatccac
     6241 gacttcggac gctccggcga cgtcgtctac gccgtcaccg agctcctcga cggcgagact
     6301 ctccgcgagc ggctccgtca cgggcgcccc tcctggcgaa agtcggccga gatcgccgcg
     6361 gccgccgccg acgggctcgc cgctgcccac gcccggcacc tcgtccaccg cgacctgaag
     6421 cccgagaacg tcttcctcac ggcggacggt cgcgtgaagg tgctcgactt cggcctcgcg
     6481 aaggtcgtcg agccggtcct ccccgaggcc tccaccctca cctcgcccgc gagtacggcg
     6541 gaggggcgcc tcctcgggac cgtcgcgtac atgtcgccgg agcaggcccg cggccttggc
     6601 gtcgactccc gctccgacct cttctcgctc gggacggtcc tctacgagat gctctcggga
     6661 cggcgcccct tcgagcgcgc gaccgcggcc gacacgatct ccgcgatcct gcacgaggac
     6721 cccgatccgc tcgacgcgat cgcggcgatc ccgccggcgc tcgccgccat cgtcgcccgc
     6781 tgcctcgaga aggagcccga ggagcgcttc cactccgcgc acgacctggc gatcgccctc
     6841 cgcagcctct cggccgagaa cctctccggc gcccgtcccg tccgctcgcg gcggcggccc
     6901 gcgcgcgccc gccccgcgct gctggcggcg gccgccgtcg ccgtcctcgc agcggccgcg
     6961 ctgctcgtgg ccgatcgcgc gcgagagcgg cccggccgcg agacggtcca ggtcgtcgca
     7021 cggcccgtca cctcgatgcc cggctgggag atcgagcccg ccgtctctcc ggacggcagc
     7081 ctcgtggcgt tcgcctcgaa cgcggcgggg agcgcggacg tctggctcgt cgagagctcg
     7141 ggcggcgagc cgcggcagct gacggacgtg gggatccccg gcgacgacgc gcccgaggac
     7201 cgctccccgg cctggttgcc cgacgggcgc tcgctcctct tctcctcgga tcgcaacggc
     7261 agcagatcgc tcttccgcgt ctccttcctc ggcgggagcc cgagcctcgt cgtggaggac
     7321 gccgccgagc ccgccctgag cgacgacgga accgtcctcg ccttcacccg caccgtcggg
     7381 aacgggcgct accggcggat ctgggttgcc ccgctcgcag cacccgacga cgcccgggcg
     7441 atcacgacgg acgaggacgg cctgtgggac cacgcccggc cggcgctctc ccccgacggc
     7501 cgaaggctct gctacgccga cttccggaac ctctggatcg tcgacctgcc cggcggcagg
     7561 ccccgcccgc tcaccgccga cgacgcgttc gacgccgagc cccgcttctc gcccgacggg
     7621 cgtcacgtct acttcacgtc tcgtcgcggg gacgtcgtgg cggtctgggc cgtcccggcc
     7681 gacggcggcg ccgcgacgag gatcaccggc gaggcggcct cggagaccca tccctccttc
     7741 tcccgcgacg ggagcgtcct cgtgacggcg acgcgcgtgc gcgactccga cgtcgccgtc
     7801 ctcgaccgcg tcgcgaagac cgtcgcccgg atctcgagca cgctgagcga ggagacgccc
     7861 gtcctcgagc cggacggccg ggccgtctac ttctcctcca accggaccgg ccgctcgaac
     7921 ctctggaagc agccgctcca cgctggccga tccgtcggac cggcgaaccg cctcacgtcc
     7981 ttcgaggccg ggggcccggc gacgccgtcg atctcgcggg acggccgttt ccttgcgttc
     8041 ttccgggccc tctccgggaa ccgcgacatc tggatcctcc cgacggccgg cggcatcgcc
     8101 cggcccctcg tcggcggccc cgatcgtgag ttccacccgg cgctctctcc ggacgcccga
     8161 cagctcgtct tcgtctcctc gcgaggcggc gtcgagcatc tctacacggt ccccctccgc
     8221 gacggcgccg ccgaaggcga gccgcggccg atcaccttcg gcgcctcggc cgacgccttc
     8281 ccggttctct caccggacgg ttcgcggatc gcgttcctcc gggacgggga gatccacctc
     8341 atcgactccg aaggacgcgg cgagcccgag cgcctgacgc acgacgcccg gccgaatcac
     8401 ttcgcctggg acgccggcgg cgacgcgctc gtcgtcgccg gtcgtttctc cgggcgcagg
     8461 ctcgtcctcc gccgcgtccg cctcgacggc gtcgtcgagc cgatcgagcc ggaggtcgag
     8521 ctcggcgggg agctctcctc gggctacctc tccgtgagcg ccgacggccg cttcctcgcc
     8581 gtccaggtcg gccggctgga ggccgacgtc tgggtccggg ctcccttcac gggcggaccg
     8641 tgaccgatga acgggcgccg ggaccgccct cggcgcaccc tcgaccgagc gcagacgacg
     8701 aaaggagatc ggaatgccca cgacgctcgt ccaccgcctc gagctgatga ggctcctcca
     8761 ccgcttcaag gggctcgtcg agtgggagct cgagaagctc gccgcgctcg acaccaggac
     8821 gatctccctc gagctcgagc ctccggcctt cgcggtacgg aagcccgacc ggaagccacc
     8881 tccgatcgcg acggggacgg agcccgcggc ggaggcgccg gcggtctccg agactccttc
     8941 cattccggga gccgacgcgc cggacggatc gctcgccgac ctctcacgcc cggccggagc
     9001 gccgtcgcac ccgttgcaga agacgaagga ccccacgaac ctcgagagct gctgccgatc
     9061 ggccggcctt ccgaccggcg actacgcgga gctcgtcgac gcggcgaccc cgtacgtccg
     9121 ggcgctcgcg gaggagtgac gcgcatcgac gatcgcgccg tccggccgct cgtcccgggc
     9181 ccgcgcgccg tgacgctcct gctcacgacg cggtgcaacc tctcgtgcgc ctactgcccg
     9241 cagcggcgcg gcgcgccccg cgagatggac gccgccgctc tcgaggccgc cgtccgcctc
     9301 gtggccacgt ccggccacgc gcgaccaaag ctcgtcctct tcggcggcga gccgctcctc
     9361 gcgaaggcgc tcgtccggag ggtgctggcg ctcctcgcgg catccgcgcg cccgggcctc
     9421 gcgcccgacg tgcacgtcgt gacgaacggc ctcctcctcg acgcagagac ggccgcgtcc
     9481 ctcgacgagg cgggcgtcac gatcgagctc tcctgcgatg gcttcgacgc cgcgcaggag
     9541 cgccgcggcg ccggcaccgg cgccgatctc cgctccgtct tcctcctcct cgcgcggagc
     9601 cgccctcgcc tcctcgcgga gcggctcgtc gcgcgcgtga ccgtcgactc cgggaacgtc
     9661 ggcgcgctcg gccgatcggt cgcggagctc cgcgcggcgg gaatccgcga gatccgggcc
     9721 gcgccggtcc agacgcccga cccgggatgg agcgaggcct ccgcggacct cctcgaccgc
     9781 gccctcagcg agatcgccgc tctccctccg aacgggaacg gggactcgac cttcgatccg
     9841 ctccggcctt cggcgggccc tcggaacccg ttcggcgtcg ccgcgtgcag cctcggcgag
     9901 gccgacgtcc tcttcgtcga cgtcgacggc cgcatcgccc cgtgcggagc cttcgcaccg
     9961 tccgtcctgc cgtcactccc gcccctcgcg gaggagcttc ggcagcggct cgtcgggctg
    10021 cgggtggacg acccggcgct cgacacgcgt ctcgcgctcc gcgccgcggg gaggcagagc
    10081 ctcccggcgc tcgtcgcctc ggcggcgcgt cgctctcccc gcggcccctg cgcctcctgc
    10141 gaggcgctcg acgagtgcgc cgtctgcccc gccgcgatcg ccggcgcgcc ggagcaggac
    10201 ccggacctcg tcccggcgat ctggtgcgac tggaaccgcc ggacggcccg gcaccgccgc
    10261 gcgagcctcc ggagcgccgt cgacgatcag cgcgcgggca gcagctcgag cgccccggag
    10321 acgagcgccg tcaccgcggt ccggatcgcg ggagcgtagg ccggggcgaa ctcggccgag
    10381 tggagcggcg ggagcgacgt cccgtcggcc ttcgccttcg cgagcgccgc gggctccgcg
    10441 acgccgagcc agaagagcga ggcggggacg ccttccctcc cgaagtagga gaagtcctcg
    10501 ctcacgctcg ccggagggaa ggaggcgacg ttcgcggcgc cgagcgccgc ggtaaaggcc
    10561 gtgcgcagcc tctccgtcag ggacgcgtcg ttcacgacgc ccggcgtcga ctcgacgatc
    10621 gagaagtcgg gcgccttcgg cgcgttcccc gcgatcgact cccccgtcgc gacgcgccgg
    10681 atcccctcga ggagccgctg cctcacctcc tcccggtagg cgcggaccgt gatcttcagc
    10741 cgcgcctcgg gcgggatgac gttgtgcttg gcaccggcct ggaacgagcc gaccgagacg
    10801 acggccggct cgaacgggtc cttctctcgc gagacgagcg cctggagcgc gacgaccgtc
    10861 cgggccgcga tgaggacggg gtcgaccgtc cggtgcggca tcgccccgtg cccccccttg
    10921 ccgtggatcg tcaggtcgac cgagtcgacc gaggcgaagg ccgctcccgg cgttacgccg
    10981 accgagcccg ccgggagctc gggcgtcacg tggagcgcga gggcgaagtc gggccgcggg
    11041 aaacgggaga agagcccgtc cttcagcatc ttcgcggccc cggccccctt ctcttcggcc
    11101 ggctgcccga ccatcacgag cgtcccgcgc cagctcccct tcgcgcgcgc gaggagcgtc
    11161 gccgcgccgg tccaggcggt catgtggagg tcgtgcccgc aggcgtgcat gaccggggtc
    11221 ggcgtgccgt cgtccgcctt tcccgtcgcg cggctcgcga acgacacgcc ggtcttctcc
    11281 tcgacgggca gggcgtcgag gtcggtccgg agcatcacga ccggtccgtc cccgttccgg
    11341 aggacgccga cgatcccggt cccgccgacg ccttcggaca cctcgtaccc gagcgcgcgc
    11401 agccgcccgg ccatcttcgc ggccgtcgcc gtttcctgca tcgagagctc cggcgtcgcg
    11461 tggagctccc gatagagctc gccgagcggc tccgtctcgc ccgccagcgg cgcgaggacg
    11521 gcctggaggc gcggcgcggg gggcgccgcg agggcgggga cggcgaggcc ggcggcgagg
    11581 gcggcagcgg ctgggcgcat cggatcctcc atggggtctc tcccgcctcc gactatagtg
    11641 gaggggtgag cgccgagacg cccggctggg tccgcgacgc cgtcttctac caggtcttcc
    11701 ccgaccggtt cgcgcggagc gcgcaagtgg cgaagccggc gaacctcgag gcgtgggact
    11761 cacctccgac cgcgcacgga ttcaagggag gcgacctcct cggcctcgcc gagaagctcg
    11821 acgacctctc ggcgctcggc gtgaccgcgc tctacctcaa cccggtcttc gcctccgcgg
    11881 cgaaccaccg ctaccacacc tgggactacc accaggtcga cccgatcctc ggcgggaacg
    11941 aggcgctgcg cgagctcctc gacgcggccc acgcgcgggg gatgagggtc gtcctcgacg
    12001 gcgtcttcaa ccacgtcggg cgcggcttct tcgcgttcca cagcgtcctc gagaacggcg
    12061 ccgactcccc ctacctcgac tggttccacc tcgacctcga gcgcctgcag gacggacggc
    12121 cgctcggcgc ctacgccgag ccgaagctcc tgcgccgctc gaagaagccg aagcccgcgt
    12181 tcgaggagct cggctactcg gcctggtggg actgccccgc actgccgaag ctcgacacgc
    12241 ggacgcccgc cgtccgggag tacctctggg acgtggccga gcgctggatc cgcttcggtg
    12301 cggacggctg gcgcctcgac gtcccgtcgg agatcgacga cgacgagttc tggcgcgagc
    12361 tgcgccgccg cgtgaagcgc gcgaaccccg aggcctacct cgtcggggag atctggcacg
    12421 acgcccgccg ctggctcgcg ggggaccagt tcgacgcgac gatgaactac ccgctcgcga
    12481 aggcggcgct cggcttcttc ctcccggagc tggagacgga cgccctgcgg tcggtcggag
    12541 gctaccggga cgtcgtcgag ctcgacgccg ccgcgttcgc agcggaggtg gagcgggtgc
    12601 tcggcctcta cgacccggcc gtccgggacg cacagctcaa cctcatcggg agccacgaca
    12661 cgccccgctt cctcacgctc gcgggcgggg agctcgccgc gctcgagatg gcgttcctct
    12721 tcctcttcac gatgcccggc gccccctgca tctactacgg cgacgagatc ggcctggagg
    12781 gcggacccga ccccgactgc cggcggacgt acccgtggga cgaaggctcg tgggaccacg
    12841 tcctgcgcga gcaggtccgg cggctcgccg ccctccgccg cgcccacccc gcgctccgcg
    12901 ccggcgcgta ccggacgatc ctggccgagg acgcgatcgt ttcctacgtc cgggaggagg
    12961 agggcggcga cgccgtcgtc gtcgcgttca acgtctcccg cgaggagcgg cgcgtcgacc
    13021 ttccggtcgg agcgctcccg gaacgcctcc tcgacgcggc caccggcgac gacctcgtcg
    13081 tgaccgacgg ggagctgcgc gggatcgacc tcccgggccg ctcgggccgc gcgttcgtca
    13141 cgcctccggc cggctgagcc cttctcgccg tcgaggcgtc gcggcgtcga tcccggcgag
    13201 gcgttccgcc ccggatgagg tccctccggg cgttcggcct gatgcgtttg catcacgccg
    13261 actcgatcgc ccagaaattg cgacgagaac ccaacattcg cacaacccgc gttcatttct
    13321 ttgtttatcg aaacgaattc cggattcggg attctgaaca tcaccggcca atccgacccg
    13381 ttcgcggcct gaattgaggc gtctgaagga tggtgctgcg ctgcaacggg aaggaacatg
    13441 cgcgccggat caggcagcag gagccgtgcc gccgccaggg ggccgcgtgt cgggagagag
    13501 ccgggaagcc atgcacctcg gggaggcgtc tcgacgcctc tgcggacctc cgtgcggctt
    13561 gaccggcgcc gcgtccgtcc cgccccggcg ccgctccccg gccccggccc cgcacctttc
    13621 gcggcgaacg cagcgcaaca ggatgggaga tcgatgagcg acaccgttcg gtacgacgat
    13681 cgaaccccgc ggctcttctt cctcgccgcg atcttctggg ccgtggtcgg gatgctcgtc
    13741 ggcgtcctgg cggccgcgat gctcttcctg ccggagctga acctcgcgcc gtacttcacg
    13801 ttcgggaggc tccgcccgct ccacacgaac gcggtcatct tcgcgttcgt cgggaacatg
    13861 atcttcggcg ggacctacca ctcgatgcag cggctgctga aggcccgcct cttcagcgac
    13921 gccctgtcga agatccactt ctggggatgg cagctgctga tcgcggccgg ggcggtcgct
    13981 ctcgtcgccg gcgtcacgca ggcgaaggag tacgccgagc tcccgtgggt cctcgacgtc
    14041 gtcatcacgt tcctctgggt cgtcttcgcc gtcaacttct tcgggacgat cgcccgccgc
    14101 cgcgaggagc acctctacgt cgcgatctgg ttctacatcg cgacgatcgt cgcggtcgcg
    14161 atcctccaca tcggcaacag catggcgatc ccctactcct ggctcgggag ctactcggcc
    14221 tacgcgggcg tcaaggacgc cctgatgcag tggtggtacg ggcacaacgc cgtcgccttc
    14281 ttcctgacga cgccctacct cggcctgatg tactactacc tgccgaaggc ggccgagcgc
    14341 ccggtcttca gctacaagct ctcgatcatg cacttctggt cgctggtctt cgtctacatc
    14401 tgggcggggc cgcaccacct ccactacacg gcgctccccg agtgggcctc gacgctcggg
    14461 atgctcttct cgctcatcct ctggatgccg tcctggggcg gcatggtgaa cggcctcctg
    14521 acgctccgcg gggcgtggaa caagctccgt gacgaccccg tcctgaagtt cctcgtcgtc
    14581 gccgtgacct actacggcat gtcgacgttc gaggggccga tgatgtcgat caagtcggtg
    14641 aacgcggtct cgcactacac cgactggacg atcggccacg tccacgccgg gacgctcggc
    14701 tggaactcgt tcctctcgtt cgggatcctc tactggatcg tcccccgcct ctggaagacg
    14761 gagctcttct ccaagaagct cgccacggcg cacttctgga tcgggacgat cggcctgatc
    14821 ctctaccagg tctcgatgtg ggtcgccggc atcacccagt gggcgatgtg gcgcgccttc
    14881 gagcccgacg gccgcctcgt ctaccccgac ttcatcgaga ccgtcctccg gatcgtcccg
    14941 atgtactggg tccgcctcgt cggcgcgctc ctcttcttcg cgggcctcct cgtcatgctc
    15001 tacaacctcg tccggacgat gaggagcgcc ccggccggct tcgacgagga gcccgaggtg
    15061 aaggcgccgc ccctcgtcgt cgaccgctcg gcccccggcg cgacgacggc ccccgggacg
    15121 tgggaccacg ccttctaccg cttccagcac gtgatccggc acggcgtcca ccgcgccatc
    15181 gagggacgcg tcgtcctcct gacggtgatg acggccctcg cgctctccgt cggctccctc
    15241 gtcgaggcga tcccgctctt cttcgacgag agcaacgtga agacgatcgc gagcgtgaag
    15301 ccctacacgc cgctcgagct cctgggccgc gacatctacg tccgcgaggg ctgctacaac
    15361 tgccactccc agatggtccg gccgttccgg cacgagacgg agcggtacgg cgagtactcc
    15421 aaggccggcg agttcgtcta cgaccacccg ttccagtggg ggtcgaagag gacggggccc
    15481 gacctgcacc gcgtcggcgc gaagtacccc tcgctctggc acgtccggca catggaccag
    15541 ccccgctcca cgacgcccgg ctccgtcatg ccgcgctacc cgcacctcct gaaggacccg
    15601 atcgacgact cgctcgtcga ggcgaagatg cgcgcgatgc gcaccgtcgg ggtcccgtac
    15661 tccgacggcg agatcgcgac ggcgaaggcg gcgatcgccg cccaggcgca ggccatcgcc
    15721 tccgaggtcg aggcgcagca ggggcccccc ggcctcgccg accgggagat cacggcgctg
    15781 accgcgtacc tccagcggct cgggaccgac atccgctggc ggaggcccga ggtccagccg
    15841 ccgctcgcgc tcccggcggc tcccgccgcc gcctccgtgc cggccgccgc gcccgtcgcg
    15901 gccgcgaggt agccgccatg cttcaggagc tcgcccgcga gagcggcgcc caggcctggg
    15961 cgatcgcctc gatgctcttc ttcctcggcg tctggctcgt cctcgcggtc cgcgtcgtcc
    16021 gcgcccggcc cgacgccctc gcagcgcggg cccgcctgcc gctggccgac gacgaggccg
    16081 cctcgccggc gctcgccgcc gagccgggac gcggggcctg accggagggg acgatggccg
    16141 aatacgaaga caaggtcctc cacgacatcg acggcatcaa ggagtacgac aaccccctcc
    16201 cggggtggct catggccatc tggtgggggg cgatcgcctt ctccgtcctc tacctcgcgt
    16261 tctacgcgct ccgcttcggc gagaggtcga tggactcgga gtaccgcgcg gagacggaga
    16321 gggcgctcgc ctcggtccag gcgcacttcg acgcgaaccc gctcgtggcg ccgacctccg
    16381 ccgagctcct cgccggcgcc gcggacccgg tcgtcctcga ccggggagcg gggcgcttcg
    16441 cgaagtcgtg cgcctcctgc cacggcgaga aggcgcaggg gctcatcggc ccgaacctga
    16501 ccgacgaccg ctggatccac ggcggctccg tcgagcaggt cttccagtcg atcgtgaagg
    16561 gctggccggc gaaggggatg ccgccctggg ggcgggcgat cccgccggag gagatctcgg
    16621 cgctcgtctc ttacgtgagg agcgtccagg gctcgagccc cgcgaacgcc aagcccgccg
    16681 agggcgaccc gttcccgccc gagacgatcc ccggacgctg acgtggccgg acccgccgcc
    16741 gcgaaggagg agagagcgcg cgccgaaagg cccgccggac gggagcgcgt cttcgacgtc
    16801 gcccccgagg aggagctcct ctacagcatg tcggcggacg ggtcgcggaa gttcatccgc
    16861 ccggtcctcc accgcggccg ctactggaag atccggcgcg cgatggcctg ggggctcttc
    16921 gtcctcttcg tcgcgctccc cctcgtcccc ctgggagggc atccggccgt ccacttcgac
    16981 ctcggcaagc ggctcttcca cgtcctgggg gcgacgttcc acccgaccga caacgtcctc
    17041 ctcctcctct tcggcttcgg cgtcatcgtc acggtcttct tcgtcggctc gacgttcggc
    17101 cggatgtggt gcggctacgc ctgcccgcag accgtctacc tcgagttctt cttccgtccc
    17161 gtggagctcc tcctcgaggg ggctccgctc gcgcagcgca agctgaacgc ggcgccgttg
    17221 agcccgcgca aggcggggat caagacggcc aagtgggcga tctgggcgct cctcgcgctc
    17281 ctcctcgcct ccacgttcgt cgcctacttc gtcagctgga aggggctctt cgcgggcctc
    17341 gccgccgcgc cgggcgagat gacgggcgcg ctcgggacga tcgccttcct cacggcgctg
    17401 atcctcttcg acttcgggtg gttccgcgac cagatgtgca cggtcgcctg cccctacggc
    17461 cgcctccaaa gcgtcatggc cgaccccgac acgatcctcg tcgcctacga cgcgaagcgg
    17521 ggcgacccga aggtgaaggt gaaggaccgg gtcgcgggcg tcgccgcggg cgactgcatc
    17581 gactgccgga gctgcgtgaa cgcctgcccc accggcaccg acatccgccg cggcctccag
    17641 tcggagtgca tcggtacggc gcagtgcatc gacgcctgcg acgaggtgat gcgcggccag
    17701 aagaagccga tcgggctgat caagtacacg tccgagcgcg aggaggcggg cgggacacgg
    17761 cgcctcgtga ggccccggaa cctgatctac ctcgccctcc tgacgctcgc ctggggctcc
    17821 ctcgccttcc tcctcctgac ccgcgcggac gcccttgtcg agatcgtccg gggcggccgc
    17881 gagccatacc gcgtcctcgt caccggcgaa gtcgcgaacc agcagcggct ccgcgtcacg
    17941 aaccagctcg acgtgccgca acgcttctcc gtcgaggtcc tctcccccgg cggcgcgcgg
    18001 ttcgtcctgg cgggcggtcc cgtcgacgtc gccccggaga aggtcgtcac cgtcaacgtc
    18061 gtcacgaccg tcccggcgga ggtcttccgc gacggccagg ccacggcccg gtacgtcgtc
    18121 acgtccgacc ggggtttccg gaaggagatc gagttcctcc tcctcgggcc ctggggaaag
    18181 ggggccgcga agtgaagctc cggaagttcc tctccgagta ccgctggccc ctctacctga
    18241 cgggcctcct gacgatgtcg atcctcgcct acgcgatcct cgtctgggtg gccacgcggc
    18301 cgacggcgcc gcgcccgatc gccggcttct acgaggcctc gcaggagtgg gacgcggacg
    18361 aggcggtcgt ggcggcgagc cgcgcgctcg gttggacggt cacgtacgag ctcccggccg
    18421 gcgtgccgca cgtcgccggg acaccccgcc ccgtcgacgt tcaggtcacc gaccgcgacg
    18481 ggcagggcgt cacggggctg agcggccgcc tcttcgccct gcgcgcggcc gaggcccgcc
    18541 gcggcccgcc cggcaccctc gtcgagctgc cccaccggcc cggctcctac cgcgccctcc
    18601 tgcgcgtcga cgagccgggc gcctgggact tccgcctcga cgcgcggcgc gacggccagc
    18661 gcttcgtcca ctcggctcgc gtcgcggtcg agaaggacgc ccccgaagga gggagcgcgc
    18721 ggtgaaggga cctcgcgagc gctcgcacct gcacgccgcc cccgtggcgt gcgcgcactg
    18781 cggcctcgcg gtccccgagg ggatgctccg tcccggcgag gagaagcagt tctgctgcaa
    18841 cggctgccgg caggtccacg acctgatccg cgactggggc tacgagaagt tctacggcct
    18901 cgtcgagcag caggggggcg tgctcgagcc ggcccgcccg accgggcgcg cgttcgagga
    18961 cctcgacgac ccgagggcgc tggaaggttc gagcgacgag gagcccggag gccgctgccg
    19021 gacgcgcctc tacctcgagg gggtccactg cgccgcctgc gtctggctgg tcgagaagct
    19081 cccggaagcg ctcgacggcg tcgactccgt gcggctcaac ctcgcgagcg cggtcgccga
    19141 ggtgacgtgg cgccccggga cggtgaagct ctcggcgatc gcccgggcgc tcgaccgcct
    19201 cggctactct ccgcacgtcc accgggccgg cgccgcccgc gacgcccgcc gcgccgagga
    19261 ccgcgccacc ctcgtgaagc tcggcgtcgc cgcggccggc gcgatgaacc tgatgttcct
    19321 ccacggggcg ctctacgccg gcgaggcctc cgggatggcg tccccttacg agacgttctt
    19381 ccgctgggtc tccctcggca tcgcgctccc ggtcctcgcg ttctcggcac gcccgttctt
    19441 ccagaccgcg tacgcggggc tgaagtcccg cttcgtccac atcgacctcc ccgtcgcgat
    19501 cgggatcgcc gtcacgttcg ccgcgagcgc cgcgaacgtc gtccgcggga gcggcccggt
    19561 ctacttcgac tcgctcgcga tgctcgtcgc ggccctcctc ggcgcccggc aggtgcagcg
    19621 cgccgcgcag cgggcggcgc tcgagcgggc cgacagcctc cgcggcgtcg cgttcctcga
    19681 gttcgcgagg cgcctcgaag gggacggtcc gctcgcgccc gccgtcgagg tccccgtcgc
    19741 ggcgctcgag ccgggcgacc gcgtcgaggt ccgctcgggc gagctcgtcc cggccgacgg
    19801 cgtcgtcctc ttcgggcgct ccacgctcga cggctccgtc ctcaccggcg agtcggcccc
    19861 cgtcccggtc gccgaggggg gcgcggtctg ggcgggcgtc acgaacctcg gggcccggct
    19921 cgtcgtgaag gtcgaggcgg ccggcgagaa aacgcgcgtc ggtgcgctcc tcgccgtcgt
    19981 cgaggacgcc ctcgcgcgac gtcccgccgt tctccgccgt accgacctcc tcgcgcggcg
    20041 cttcgtccag gcgctcctcc tcctcgccgc cgccgccctc ctcctcgccc tcccgctcgg
    20101 gacggaggca gccgtcgggc gcgtcgtcgc cctcctcgtc gtcgcctgtc cctgcgcgct
    20161 cggcctctcg gtcccgctcg ccgtctcggt cgggctgatg cgggcggccc gcgccggcat
    20221 cttcgtgaag gaccccgacg ccctcgaccg gctccgccgc gtcggcaccg tcctcctcga
    20281 caagaccggg acgctcaccg aagggaaggc gacgttcgcc cgatacctcg gggacgaggc
    20341 ggcgctcgag ctcgccacga ccctcgaagc ggagtcgtcg cacgccgtcg cccacgccct
    20401 ccgcgcctcc ctcggccgcc gcgtgaacgt cgtccgcgag gtgaccgagg tccgcgaggt
    20461 cgcgggacgt ggcatctccg gcgtcgtcga cgggcgccgc ctcgccgtcg ggaaccgcgc
    20521 ccacgtcgag gcgctcgccg ctcccgcccc gggcgagctc gcggcgcacg ccgaggcgct
    20581 cgtcgccgac ggcttcagcc cgctctgggt cgccgtcgac ggccgcgtcg tcggcgtcgg
    20641 cgggatcgga gacccgctcc gcgccgacgc gcgcgccacc gtcgacgcgc tccgcgcccg
    20701 cggcgtccgc gtgaggatcc tctccggcga cgacccgcgc gtcgtcgcct ccgtcgccgc
    20761 ccgcctcggg atcgcccccg cggacgccct cggtggccgc actccggagg agaagcgcga
    20821 cgtggtcgcc tcgctcgtcg gcgcgcgcga aggcgaggag cggcgcgccc ccgccgtcgt
    20881 catggtcggc gacggcgtga acgacgccgc cgccctggcc ctggccgacg tcggcgtcgc
    20941 cgtcctcggc ggcaagggag cctccctcgt cgcggccgac gtcgtcctga cgcgcgaggg
    21001 ggtcgccccg ctcctcgacc tcctccgcgg gtcgcggcgc gtcttcggcg tcatccgccg
    21061 gaacctcgcg ttctcccttc tctacaacgc ggcgggcgcg gccctggcgc tcctgggcct
    21121 cgtcgggccg ctcctcgcgg ccgtcctgat gcccgtctcc tcgctgagcg tcgtcctctc
    21181 ctcggccctg tcgcggccct tccccgccgc ggcgcgacgc gccgcgcgga cccacggggc
    21241 ggaggcctag gtggagacga tcttcgtcct cctcccgctc gccctcctca tcgcggggat
    21301 cgccctcgcg ctcttcatct gggccgcgcg ctcggggcag ttcgacgacc tcgagacgcc
    21361 cgccgtccgg gtcctcttcg acgacgagcc ggctcccgcc gccccgcgcc ccgcgctcgg
    21421 cgagccggcc gcttcccccg ccccgaatcc tccggagacc acctccgacg cgccgacaag
    21481 gaggaccgaa tgagccgacc gagaacgccc gtccgcatcc tgatcctcct cctcgggatg
    21541 gtcgcggcgg gctccctggc gtacgcgctg agcaacgtgc gcaaggggta cgagccgcga
    21601 cagccgatcc tcttccggca ctcgcggatg gccgggcccg ccgtgatgca gacgaacgag
    21661 aagggcgagg cggtgaacgt cgggggctac gacatcccct gcctcacctg ccacaccatg
    21721 ccctacaaag ggcggcacgc aaccgtgccg tcgaccgccg tctgcatgaa ctgccacacg
    21781 gcggtcggcc tgaaccgcga gtgggtcctg aagatgaagg actactggga ccggggcgag
    21841 ccgatcccgt gggtgaaggt ccacgacctc cccgacttcg tctacttcga ccactcggcc
    21901 cacctgaacg cgaaggacga gaagggaatg ccgaagctcc cgctgaagga cgcggagggg
    21961 cgcccctggc agatctgcgc gaactgccac gggcgcgtcg aggagaccga catcgtctcc
    22021 gtcacgaacg ccttcaacat gcagtggtgc ctcgactgcc accgcaagcc cgagatgaag
    22081 gcctcgaccg actgcgttgc ctgccaccgc tagagggaga cgaagatgag accgaacgag
    22141 acctcccccc ggcccgaggc aggcgtcgac cgacgcgagt tcctccgcct cgggagcgcg
    22201 ctcgcggcgg ccggcgccct cgcgagcgcg ggctgccagc cgccgcagga agcgacgatc
    22261 ccgttccacg acatgcccga gagcctcgcc gaggggatcg ggcgggcccg cttcttccac
    22321 accgtcgtcg acggcacgcc cgtcctcgtg aggacacgcg aggggcgccc gatcctcgtc
    22381 gccccgagcc cgaccgacgc gagcggccgg ggcctgacgc tccgccacca ggcggcgctg
    22441 atggacctct acgaccccga ccgggcccgc ggcccgctct ccgtccgccg gggccagggg
    22501 cccaccgtcg cctccagctg gaagacggtc ggcgccgacg tcgtctcccg cctcgcgaag
    22561 gccgggagcg gcgccgtcct cctgacgggg cccgtcgcga gccccgccct ctccgaggcg
    22621 atcgccgccc tctccgccgc gacgggcctc cgtcacgtcg cctggtcgcc gatcggcggc
    22681 gacgccgcgg cggccgcgca ccgcgccgcg ttcggcgacg agcggctccc ccgtccgctc
    22741 ctcgggaagg ccgacctgat cctgggcctc ggcgccgaga tcctcgatcg ccccgccgac
    22801 ggcctcgagc gcgacttcgc gatgcgccgc tcccccgacg cgcccgaagc gaagatgagc
    22861 cgcttcgtcc agctcgaagg gcgcctctcg ctcaccggcg cgaacgccga cgagcgggtg
    22921 cgcgtccgcg actcgcagct cgcctccgtc gccgccgccc tcgcccacga gctcgtcgtc
    22981 acccggaagg cggggccgct cgcgggcagc cccgaagtcg ccgccgccct cgcgccgttc
    23041 gcgcccgagg ccgtcgcgaa gagggcgagc ctcgacccgg cgctcctctc ccgcctcgcc
    23101 ggcgagctcc tcgccgcgcg cgggaaggcg atcgtcctcg cgggaggcgc ggcgagcggc
    23161 tcggcgtcgg gcgaggcgct cgagctggtc gccctcctcc tgaacctgac gctcgacgcc
    23221 tactccgccg gcctcttcga cgaggcggcc gccgagcggc ccgccgcggg tggcgccgcc
    23281 gccctcgcgg ccctctccga cgagatgcgc gctgggaaag tctcgctcct cctcgtcgcg
    23341 ggcgcgaacc ccgtctacga cgcgcccccg gccgtccgct tcgccgaggc gatcgcgaag
    23401 gtccccttcg tcgtctcgct gaacgaccgc ctcgacgaga cgagcctcct cgccgacctc
    23461 ttcgccccgg cgagccaccc gttcgagtgc tggagcgacg cggcgctccc gaaggggctc
    23521 ctcgcgatcc agcagccggt gatccagccg ctctacgaca cgcgcggcct cctcgacctc
    23581 ctcgtcgagt ggggcgccgc cgccggggcg ggcggagagc tcgccgccgc cgtcgccgcc
    23641 gccacggccg cgctcgcgaa ggccccgctc cccgcctccg cggctcccgc gccgagcccc
    23701 agcttcgcgt ggcacttcct ccgcgccgcc tgggcgcccc gcctcggcgc ggcccccggc
    23761 tcgcccgagc tcgagaaggc ctggaacgag ctcctccgcg ccggcgcctt ccaggggccc
    23821 gcctccgcgg cgcctcccgc gcggacgctc gccctggcgg ccctcgcgct cctctcctcg
    23881 atcccggaag cgcccgccgg cctcgagctc cagctctacc cccacctcgc gctgcacgac
    23941 gggcgcgccg ggaacaacgc ctggctccag gagttccccg acccgatcac gcgcatctcc
    24001 tggggtgggg cgctctcgat cgccccccgg cgcttcgacg agatgggcct cgcgaacggc
    24061 gacctcgtcg aggtcgacgc ggggcacgcg aagctcgtcg cgccggccta ccgccacgcc
    24121 gggatgcacg ccgacgaggt cgcgctcccg ctcggcctcg gccgcgcctc ctgcggcccg
    24181 atcggcgccg gcgtcgggcc gaacgccttc gcgctcggcc tcctgacggg cggccgcctc
    24241 ctctccgccg gcctccccgt gaagatccgg aagttcggcg ggcgcgagga gctcgcgttc
    24301 gcccagggct ccgacgtcat cgaccgcgac cgccgccccc tcgtcccggt cacgaccctc
    24361 tccgcctacg agaaggaccc gaagtcgggg accgagcagc agcacggagg cccctcggcc
    24421 tggcccgagc acgactaccc gaacgcccgc tgggcgatgg cgatcgacct cagcaagtgc
    24481 aacggctgcg gcaagtgcgt cctcggctgc caggccgaga acaacatccc ggtcgtcggc
    24541 cgccggggga tcgtcgacgg ccgcgagatg tcgtggatgc ggatcgaccg ctactacgac
    24601 gccccgaaga aggaaggccg ctgggacgcc gaggtctggg acggcccgct gccggtcgtc
    24661 gaggagccga aggtcctctt cgagccgatg ctctgccagc actgcgagaa cgcgccgtgc
    24721 gagaccgtct gcccgttcgt cgcgacgatg cacagcgagg acggcctgaa ccagcagatc
    24781 tacaaccgct gcgtcggcac ccgctactgc gcgaacaact gcccgttcaa ggtgcggcgc
    24841 ttcaacttct tcgagtacgg caaggcccag gagagcgccc tcttccgcgc cctcgagccg
    24901 cgcatcaagc ggaacgcgga gctgaacacc cgctccccga tgcagatgaa gaacaacccc
    24961 gaggtgaccg tgcggagccg cggggtcatg gagaagtgct ccttctgcgt ccaaagaatc
    25021 cgcgaggcgc gggccgcggc gacgcgcgac ggtaaggaga agacgcactt ccccgacggc
    25081 gccgtcgtgc cggcctgcat ggaggcctgc ccgaccggcg cgatcacgtt cggcgacgtg
    25141 aatgctcccg gctcgaaggt cgccgcgctc tccgcccacc cgcgggcaat gcgcctcctc
    25201 gacgcgttgg gcgtcaagcc ctccatctcc tacctgacga aggtgcgcaa tgacaaggcc
    25261 tgaggcgagc ccctacgcca cggtgatgga cgacagccag ctcacgggcc ggccgatcgt
    25321 cctcgcggga gccggcccct acggcggcga cctcgaggtc cccgagccgc tcgtgaccgg
    25381 cgccaagaag tggagcgacg tcgaccagga catctgcatg cacgccgagc gcttccccac
    25441 gaagaggtgg tgggccgcgt tcgccgtagc gctgacgctc ctcggcgtcc tcgtcgtctc
    25501 gctcgtcacc ctcctctacg ccg
//
LOCUS       DAOONL010000047        24963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1872_length_24963_cov_14.800426, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000047 DAOONL010000000
VERSION     DAOONL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24963
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1872_length_24963_cov_14.800426"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="tuf"
                     /locus_tag="PLP50_08880"
     CDS             <1..1028
                     /gene="tuf"
                     /locus_tag="PLP50_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPA51704.1"
                     /translation="RDATKILTVALAHVEYETEKRHYAHIDCPGHADYIKNMITGAAQ
                     MDGAILVVEAPKGPMPQTKEHVLLARQVNVPAMVVFLNKCDLMEDPELLDLVELEVRD
                     LLNKYEFPGDTITVVRGSAVKALNDPTGPDAQCIWELAKALDENIPDPVRETDKPFLM
                     PIEDVFSISGRGTVVTGRVERGVVKVGEEIEIVGFKDTVKKVVTGVEMFKKLLDQGQA
                     GDNIGVLLRGVERTDVERGQVLCKPGTIKPHTKFMGKIYVLSKEEGGRHTPFFNNYRP
                     QFFIRTTDVTGSAKLPEGVEMVMPGDNVELEISLIAPIAIEKGLKFAIREGGRTVGAG
                     TVTEILA"
     gene            1117..1434
                     /gene="rpsJ"
                     /locus_tag="PLP50_08885"
     CDS             1117..1434
                     /gene="rpsJ"
                     /locus_tag="PLP50_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPA51705.1"
                     /translation="MGNDKIRIRLKGYDYRILDQSTLEIVDTARRSGAKVAGPIPLPT
                     AISRWTVNRSPHVDKKSREQFEMRTHKRLLDIFEWTPQTVDALTKLELPAGVEVEIRT
                     MGK"
     gene            1463..2110
                     /gene="rplC"
                     /locus_tag="PLP50_08890"
     CDS             1463..2110
                     /gene="rplC"
                     /locus_tag="PLP50_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPA51706.1"
                     /translation="MITGILGRKIGMTQLFAADGTVVPVTVVEAGPCLVVQRKTKQND
                     GYDAVQLGLVGPRPSPRRLTKAVRGHFEKAGVPPTRTVGEVRCEEGDAFAPGDKVLCD
                     IFNAAEHVDVVGVSKGKGFQGVIKRHGFGGGVATHGSMFHRLPGSIGASAFPSRVIAG
                     TRSSGRMGGKQVTTKNLEVVKVDAENHLIYLRGAVPGARNTVVKIVKKPAKTQAA"
     gene            2122..2778
                     /gene="rplD"
                     /locus_tag="PLP50_08895"
     CDS             2122..2778
                     /gene="rplD"
                     /locus_tag="PLP50_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPA51707.1"
                     /translation="MATTKKTSPASGLTVPIRNLNAETVGEANLPEEIFGVPFRRHVV
                     WEVVRAIRAREHAGTHKTKVRSEVSGTGKKPFKQKHTGRARQGGGRPPIHRHGGTVHG
                     PVPRSYELKVNVATKKAALRCVLSEKLRENQLVLLESLELPTHRTKDLAAAMTKLGVT
                     GKALLLDRRENEKLAKAARNNPLLLVEDALGLDAYAALEAGTVVLTEQALKTVTEVLG
                     "
     gene            2775..3068
                     /gene="rplW"
                     /locus_tag="PLP50_08900"
     CDS             2775..3068
                     /gene="rplW"
                     /locus_tag="PLP50_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPA51708.1"
                     /translation="MKTAQSILLKPIITEKSTTLREAQNVLTFKVAPDANRIEIRRAV
                     EVLFDVKVDSVRVLKTTSKIKKVGRSIGRTSEGRKAYVKLKAGEKLPPIFEGV"
     gene            3070..3894
                     /gene="rplB"
                     /locus_tag="PLP50_08905"
     CDS             3070..3894
                     /gene="rplB"
                     /locus_tag="PLP50_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPA51709.1"
                     /translation="MPVRSYKPTSPGLRQRTTSDFAEITRSAPEKSLTRGKVKTGGRN
                     NLGQLTSWWRGGGHKRRYRVIDFKRDKFGIPGKVASVEYDPNRSARIALIHYADGEKR
                     YILAPAGIKVGQTVVSGPQADILTGNALPLKAIPLGTVVHNVELTPGKGGQMVRSAGA
                     GAQLMAKEGDHALLRMPSGELRRVRVLCYATVGQVGNLEHENVSIGKAGRNRWLGWKP
                     HNRGVAMNPVDHPMGGGEGKTSGGRHPTSPWGWKTKGKKTRNNKRTDALIVKRRTK"
     gene            3916..4215
                     /gene="rpsS"
                     /locus_tag="PLP50_08910"
     CDS             3916..4215
                     /gene="rpsS"
                     /locus_tag="PLP50_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPA51710.1"
                     /translation="MSRSTKKGPFVDGHLLEKIVALNSAGDKRVVKTWSRRSTIMPEM
                     VGHTVAVHNGKKFIPVYVSENMVGHKLGEFSPTRTFKGHSGRKVEKGASPAPAAK"
     gene            4226..4834
                     /locus_tag="PLP50_08915"
     CDS             4226..4834
                     /locus_tag="PLP50_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HPA51711.1"
                     /translation="MEAVARLRHYRGSAQKVRLVADLIRMKPVGRALATLRATPKGCS
                     PDIKKLVESAVANAQQNGSGVDADALVVSRVLVDGAGRKPLQRNAMLRSVSNYKKRFA
                     RPRFMSGPQGRMWLVTRPFCHVTVVLSDDPAVRRKAGLSAEPARPVAKKKGGKVAAAV
                     AKAAARPQAPKADAKPAKAEGAPKAPRTRKSAKAAEPAGGQE"
     gene            4842..5489
                     /gene="rpsC"
                     /locus_tag="PLP50_08920"
     CDS             4842..5489
                     /gene="rpsC"
                     /locus_tag="PLP50_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPA51712.1"
                     /translation="MGQKVHPYGFRIGYNRTWHSRWYAEKDYLRFLHDDLKLRAELKR
                     DLSNAAVSEVEIERGNKLKINIMTARPGVIIGKKGAAVDQLRDRLQRRLGKDIHVNIV
                     EIQRPETDAQLVSESIAMQLERRVAFRRAMKKGMESAFRFGAQGIKIRCSGRLNGAEI
                     ARSEWYQEGRLPLHTLKADIDYGFAEARTTYGKIGVKCWIYKGDLHRAKSRRKVA"
     gene            5502..5927
                     /gene="rplP"
                     /locus_tag="PLP50_08925"
     CDS             5502..5927
                     /gene="rplP"
                     /locus_tag="PLP50_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPA51713.1"
                     /translation="MLMPSKVKYRKQQKGRRRGTALRGGTLSFGEFGLQALEAGWLTA
                     RQIEAGRVAIQRHVKRGGKLWIRVFPDKPITKKPLETRMGKGKGAPEAWVSVIKPARI
                     LFEMEGVDEKIARDAFRLAADKIGIKTRFVSRSFEEAVR"
     gene            5924..6118
                     /gene="rpmC"
                     /locus_tag="PLP50_08930"
     CDS             5924..6118
                     /gene="rpmC"
                     /locus_tag="PLP50_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPA51714.1"
                     /translation="MKGTTVETMKGWSADERGAKLREWTQALFNLRLQKATGQLENPM
                     KIRQLRRDIARLKTAERQAR"
     gene            6115..6408
                     /gene="rpsQ"
                     /locus_tag="PLP50_08935"
     CDS             6115..6408
                     /gene="rpsQ"
                     /locus_tag="PLP50_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPA51715.1"
                     /translation="MTTETTTAAAKPAARKSRKVGTVVSNKMEKTVVVEVTRLVKHGR
                     YGKFQKKSARFLADDRVHACIPGDVVEIEETRPLSARKRWRVRAVVTAARREG"
     gene            6421..6795
                     /gene="rplN"
                     /locus_tag="PLP50_08940"
     CDS             6421..6795
                     /gene="rplN"
                     /locus_tag="PLP50_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPA51716.1"
                     /translation="MIQMGTILEVADNSGAKRLSCIRLKGGSVGQYGRLGDVITCAVR
                     ESAPDAPDAVKKGKVVKAVIVRTRREQRRRDGSYIRFDNNAAVLINNDKEPIGTRVFG
                     PVARELREKKYMKIISLAPEVI"
     gene            6806..7174
                     /gene="rplX"
                     /locus_tag="PLP50_08945"
     CDS             6806..7174
                     /gene="rplX"
                     /locus_tag="PLP50_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPA51717.1"
                     /translation="MKKKQPEYTGKTRVRKGDEVVVLAGKDRGAHGKVLRVVPDKGRV
                     VVEGVNLIKRATRPNPQKNIKGGIVEREAAIAISNVMLRDPETGKPTRVGVRVEGGER
                     VRYARRSSTTISSVTKKTGA"
     gene            7178..7720
                     /gene="rplE"
                     /locus_tag="PLP50_08950"
     CDS             7178..7720
                     /gene="rplE"
                     /locus_tag="PLP50_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPA51718.1"
                     /translation="MSSRLNEKYRKDVVPVLKKEFGIDNPMAVPKIEKVVLNMGVGEA
                     ITNVKIVEAAAEELTKIAGQRAVITRARKSIATFKLREGMPIGCRVTLRGERMYEFLD
                     RLLNIALPRVRDFRGVPTRAFDGRGNYTLGIRDHLVFPEIDPGKVDKSKGLNVTIVTT
                     AGSDDRARVLLRELGMPFGR"
     gene            7740..7925
                     /locus_tag="PLP50_08955"
     CDS             7740..7925
                     /locus_tag="PLP50_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPA51719.1"
                     /translation="MARRSSIVKTTRKPKFAIRTHNRCKICGRPRSYIRKFGTCRICF
                     RNLALGGYVPGVTKASW"
     gene            7936..8337
                     /gene="rpsH"
                     /locus_tag="PLP50_08960"
     CDS             7936..8337
                     /gene="rpsH"
                     /locus_tag="PLP50_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPA51720.1"
                     /translation="MAALTDPIADLLTRVRNGLTSRHEKIDVPSSRLKIEIARILKDE
                     GYIKNFKVLDDKGAGLLRLYLKYDDAGNPVIHGLTRVSRPGRRLYRGKDELPRVLGGL
                     GIAIVSTSQGLLTGHDARKRGLGGEVVCSVW"
     gene            8354..8911
                     /gene="rplF"
                     /locus_tag="PLP50_08965"
     CDS             8354..8911
                     /gene="rplF"
                     /locus_tag="PLP50_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPA51721.1"
                     /translation="MSRVGKKPITLPKGVEVKVDAAGVHVKGPKGTVSTPVLEGITVA
                     VKDGRVDLVRASEEKRVRAFHGLNRALLQNAVVGVAEGYKRSIDLVGIGYKAEKKGNE
                     VIFTCGYSHTVRFPEPDGVTIEVAPDAKAKDVTHIVVTGVDKQKVGETATRIRGIKPP
                     DPYKLKGFRFVGEVLKKKAGKAAGK"
     gene            8922..9290
                     /gene="rplR"
                     /locus_tag="PLP50_08970"
     CDS             8922..9290
                     /gene="rplR"
                     /locus_tag="PLP50_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPA51722.1"
                     /translation="MNRAQARVEARERIHTRIRRKVEGSPERPRLAIRRTLKYIYAQL
                     VDDASGRTLAQASSREEAILGGKKSAASKGAAVSVGALIAERAKEKGISAVVFDRGGH
                     LYHGNVKALADSAREKGLKF"
     gene            9419..9952
                     /gene="rpsE"
                     /locus_tag="PLP50_08975"
     CDS             9419..9952
                     /gene="rpsE"
                     /locus_tag="PLP50_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPA51723.1"
                     /translation="MIDHVVHINRVTKVVKGGKNFSFSALVIVGDGKGRVGYGAGKAK
                     EVPTAIKKGIEMAKKTMISVTLKGTTIPHAVTGHYGAGRVFLKPAAEGTGVIAGGAVR
                     AILEAVGVQNILTKSLGTTNPHNVVKATFDALRNLHLAATILKARGRTAGAAAVEAVA
                     APAAEAVAAPEIPAETK"
     gene            10132..10296
                     /gene="rpmD"
                     /locus_tag="PLP50_08980"
     CDS             10132..10296
                     /gene="rpmD"
                     /locus_tag="PLP50_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPA51724.1"
                     /translation="MRQVKSGICAPVDQKLTLKGLGLRRIRQEVVRPDTPALRGQILK
                     VRHLVEVVGE"
     gene            10315..10770
                     /gene="rplO"
                     /locus_tag="PLP50_08985"
     CDS             10315..10770
                     /gene="rplO"
                     /locus_tag="PLP50_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPA51725.1"
                     /translation="MAHGLHNLGPRKGATSARKRVGRGPGSGLGKTSGKGHKGQQSRS
                     GYSGQLGREGGQMPLHRRLPKRGFKNIFRKEWNTVPVEALERLFAAGATVDAEALGKA
                     GLLKRKGAEVKILGTGDLKKTLIVVAHQFTEGARKKIEAAGGRCEDVPA"
     gene            10770..12173
                     /gene="secY"
                     /locus_tag="PLP50_08990"
     CDS             10770..12173
                     /gene="secY"
                     /locus_tag="PLP50_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPA51726.1"
                     /translation="MVLESFRNIFAVPDLRKRVLFTFGLLAIYRIGAHIPTPGLDALA
                     LEEFFKASAGGLLGFLDMFSGGALRRLSVFALGITPYITASIILQLLTVVWPYLEKLS
                     KEGEMGRRKITQYTRYGTVLLSVVQGAGIALWLWGTTTPTGKFLVLRDPTSEGGKIPF
                     VLMAILTLTTGTAFVMWLGEQITERGIGNGISLIIFAGIVVGLPTAVWHSFRQIQRGQ
                     MSLFTALLLGVFMLAVVAVIVFVERAQRRIPVQYAKRVVGRRVYGGQNTYLPLRVNTG
                     GVIPIIFAVSIIQFPQYLASAFKSDFMKKLADQLAMGQPLYNLFYALAIIFFCYFYIS
                     IIFNPTDTAENMRKYGGFIPGIRPGKATSDYIDSVLTRITLIGSLYLVVVALIPEFLL
                     MGFKVQSIPGIGPWLDANLPTFITQGLGVPFYFGGTSLLIVVGVAMDTVQQIESQLVM
                     RNYDGFLKKGRIRGRRS"
     gene            12178..12768
                     /locus_tag="PLP50_08995"
     CDS             12178..12768
                     /locus_tag="PLP50_08995"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPA51727.1"
                     /translation="MPVDVVFFGPPGSGKGTQASRLSSTLGIPQVSTGDLLRSNVARG
                     TDLGKIARPIMESGALVPDDLVTRMLKERLEEPDAATGALFDGYPRTVPQAEELDRLL
                     RIEGRSLTAVLFIDVPDPVIVGRLVKRAEIEGRADDTPETVAARLRVYRDKTEPLAEL
                     YRKLGLFHRIDGDRSVDEVALDVLATLNRVTGRVLA"
     gene            12765..13625
                     /gene="map"
                     /locus_tag="PLP50_09000"
     CDS             12765..13625
                     /gene="map"
                     /locus_tag="PLP50_09000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPA51728.1"
                     /translation="MITCKGRAELLKMERATWIVQQTLADCVAACRPGVTTEEIDRIA
                     ADGIRKRGGKAAFPGYRGYPKTVCSSVNDEVVHGIPTSKRKLRDGDIVGIDLGVIVDG
                     YFGDAARTAIVGEVPAPVAELVRQTWRALVAGVSAVRVGGRIGDIGAAVEAVANEKGF
                     GVVREYVGHGIGTALHEEPQVPNYGPAGKGERIREGMTLAIEPMFNLGTARVRLGPDG
                     WTVRTADGKPSAHFEHTVVATADGPVVLGFGRYTSDGLVTGVPDETDYPVPAASPRGE
                     PAETPGESRV"
     gene            13649..13867
                     /gene="infA"
                     /locus_tag="PLP50_09005"
     CDS             13649..13867
                     /gene="infA"
                     /locus_tag="PLP50_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPA51729.1"
                     /translation="MSKEDAIEVTATVIEPLPNAMFKVELENKHQVLAHISGKMRKHF
                     IRILPGDKVLVELSPYDLNRGRIIYRYK"
     gene            13929..14042
                     /gene="rpmJ"
                     /locus_tag="PLP50_09010"
     CDS             13929..14042
                     /gene="rpmJ"
                     /locus_tag="PLP50_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPA51730.1"
                     /translation="MKVRASVKKICSKCKVIRRAGVVRVICENAKHKQRQG"
     gene            14055..14438
                     /gene="rpsM"
                     /locus_tag="PLP50_09015"
     CDS             14055..14438
                     /gene="rpsM"
                     /locus_tag="PLP50_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPA51731.1"
                     /translation="MARISGVDLPPNKRVEIGLTYIFGIGRPTARMILKDTGVGPDTR
                     IKDLSEDEVAKIRKKIQDDVKVEGDLRKETAQDIKRLMDIGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRGAVAGKKKATKK"
     gene            14454..14855
                     /gene="rpsK"
                     /locus_tag="PLP50_09020"
     CDS             14454..14855
                     /gene="rpsK"
                     /locus_tag="PLP50_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPA51732.1"
                     /translation="MAAAEAKGRKKGPKKEKKNVPHAVATVQASFNNTIVAITDPSGN
                     VLAWSSAGRIGFKGSRKGTPFAAQLAAGNAAQLAQEHGVRTLDVRVQGPGAGRESAIR
                     ALAAAGLDIRSIKDATPIPHNGCRPRKRRRV"
     gene            15058..15684
                     /gene="rpsD"
                     /locus_tag="PLP50_09025"
     CDS             15058..15684
                     /gene="rpsD"
                     /locus_tag="PLP50_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPA51733.1"
                     /translation="MARYRESVCRLCRREAMKLFLKGDRCFTNKCAIERRNFPPGQHG
                     KRRSKILGYGIQLREKQKLKRFYGLLETQFRLTFEKAERMRGVTGENLLSLLERRLDN
                     VVHRLGFAASRQQARQLVRHGHVAVNGRKVDVPSFVVGPGAVVSIKEKSRQNPGVVAA
                     VDSAKGRGVPEWLELDSTEFKGTVKSLPRREDVSLQVNEKLIVELYSK"
     gene            15713..16684
                     /locus_tag="PLP50_09030"
     CDS             15713..16684
                     /locus_tag="PLP50_09030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPA51734.1"
                     /translation="MMWKGFQRPNRLEVDEESGNYGRFTAQPLERGWGTTVGNALRRV
                     LLSSIEGAAITAVKIDGVEHEFTAMPGVVEDVTDVILNLKSLAIRMHDDGPRMLVLDA
                     KSKGVVTADAFEDDAQVEILNPEATVATLADGGRLRLEATVTKGRGYVPADRNFDAEA
                     PIGTIPLDSAHSPVRRVNYHVEAARVGQATDYDKLVLEVWTNGTLTPRDAVGIAAVLL
                     REHLAIFVNLETEEGAAASGPEIPQSELDALLDQNVEELELSVRSANCLKNAGIRTLR
                     ELVQKTEKEMLETKNFGRKSLNEIKEILREKGLAFGMKLETPTGAGR"
     gene            16696..17346
                     /gene="rplQ"
                     /locus_tag="PLP50_09035"
     CDS             16696..17346
                     /gene="rplQ"
                     /locus_tag="PLP50_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPA51735.1"
                     /translation="MQHNRAGRKLGRTSAHRKALFRNQLDSLILHERIQTTISKAKEL
                     RPLAEKLVTLARRGGLHARRLALRDISQEAAKRLFDEIAPRFATRPGGYTRILKLGQR
                     PGDAAEKAIIEFVDFDYAQRRAEKKAAEKAVTEKKESLLEKARRLVTGKGGEKDGAKA
                     DEGAEAAKADEKKAEKKAAAPKAAKPAKTAAPKAPKPKTEKKGGSTPRTPRKAGGA"
     gene            17351..17815
                     /locus_tag="PLP50_09040"
     CDS             17351..17815
                     /locus_tag="PLP50_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51736.1"
                     /translation="MFPGSPPPSGGGLPIAALLPAAIDPGPAMRAFLFVSLWTVVIVG
                     GLPRRTQEALQFRFGWWDAKLAGLFSSALETFAGYRLMRVTLAAFSAGQVSVTATVIL
                     CAIAAFLLLEGGIRLLVTYRLDDLAVPSLPVWAVARLVGRLGPKGRGDSPEA"
     gene            complement(17822..18256)
                     /gene="acpS"
                     /locus_tag="PLP50_09045"
     CDS             complement(17822..18256)
                     /gene="acpS"
                     /locus_tag="PLP50_09045"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPA51737.1"
                     /translation="MTTDARSALVAGAYPHGEAVLGLGVDLCEIARIEAALARHGSRF
                     LERVCVPGEVRRPLRSRRIGEHVAGLFAAKEAAMKALGTGMQGVAFREIAVARDSGGP
                     PRLVLTGRARERADALGVVGAHVSITHGTREAVAVVLLLGGR"
     gene            complement(18253..18501)
                     /locus_tag="PLP50_09050"
     CDS             complement(18253..18501)
                     /locus_tag="PLP50_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51738.1"
                     /translation="MSEKRSGRFLQLLLGLYFLEAGLLLLLAPWSTFWVRRVVLPSPV
                     SLEPLLAAPSFRGFVAGLGVLHLVLAARVLLGRAEGSA"
     gene            complement(18581..20953)
                     /locus_tag="PLP50_09055"
     CDS             complement(18581..20953)
                     /locus_tag="PLP50_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPA51739.1"
                     /translation="MTTRILRPALAIAFLLTAVPALAQREGYSYLSWVGSEVTLVSSA
                     EDDSTARPNTPILVGDRISTGSTSRAEAILADGNILRIDVGTELRFDRLARTYESEDD
                     RNSIYLERGGVHLEHRWSTSRDEATRIDTADVTVLFPDKGLLRVETGRRGTEVYVLTG
                     RAEVAARSGRATLRAGQYAFVSGDSELEVDRLGEPRDRFAAFVSDRRAYSDDRSASDD
                     VAAGYEYDYAVADFDRYGSWITVGGSRAWRPSVAVGWRPYTDGYWRWSPVGLTWVSYE
                     PWGWLPYHYGSWSWDVGFGWYWTPGIRYSPAWVYWSYTPSWVGWCPIGYYGSYYHSYY
                     DRSHRNRRGSERWTHAYPHLRGRIDLGRIDPRGWSYTSVSRLGSRFDSRRDVLGHESV
                     RFQPGERGLVATTPLRIERGRGGSVTTVVQEAVRRVPRTLDAGPRLGSGLDDLTPVLR
                     REGTLGSTQADGLRRAFVTAGEDPSYRPVPADQLAAPRRSAGSSTATVPSRGSERGVG
                     APSTPSRTADAASPVRGSTTRTGDGWRDAGSRGETSGATRNDTEPPLRGGFGNRDVRG
                     DDGWRSPGSEGRSGSTPSRTFDVPARRAAPTVESSETPARTGTRETTPRSETWRGSEP
                     SGGSDTPARTRDAEAAPSPRRSAPAPRADEGWRGNDTPRSAPAPRSDTPPSRTSETPS
                     RGGEAPSRSSSPSRSAPAPRYEAPPSRSYEAPSRSYEAPSRPSSPQRSAPAPRYEAPP
                     SRSSSMPRSAPAPRTSAPSRSSSSSSSSRPSSPPPSRSGDSSRRSASPRG"
     gene            21138..21950
                     /locus_tag="PLP50_09060"
     CDS             21138..21950
                     /locus_tag="PLP50_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA51740.1"
                     /translation="MVFEFLKKKLQSLTSDAVSAPEPRPGAAADYEERRARAEALHEK
                     GETAEAVALLEGLAADLAGEGSFPLAVAVRHQLSSWGAGPPATQTAARDAEEMARKRE
                     ETGVVRARQTGKASAAIPEGLERSRFFAEMTKEEIAGFIASTVRRTFREGEIVLEQGK
                     TGRDLYIVTRGVLRVEAAVGEGRKLRMGVLMAGDVFGEVAFLTGRPRTATVLAETAAE
                     CLEIGGEAWDGVLAGLPRVRQVLEEVHQERARFAADVIVEELRRKREKGREG"
     gene            21951..22466
                     /gene="def"
                     /locus_tag="PLP50_09065"
     CDS             21951..22466
                     /gene="def"
                     /locus_tag="PLP50_09065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPA51741.1"
                     /translation="MAIRDIVRYGDERLVAKNEDVIDFEDPALQQLIQDLFDTGWAAP
                     GLGIAAPQIGVNRRVCVVDLSVGKDPSQRLVLVNPRVVDAEGLVRDEEGCLSFPDIVE
                     IVERPEAVAVEAYDEKGVKRVLEGADLLARAFCHEIDHLDSRLFIERMSSLKRGFVLR
                     KVLKRQKRGEW"
     gene            complement(22515..23417)
                     /locus_tag="PLP50_09070"
     CDS             complement(22515..23417)
                     /locus_tag="PLP50_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51742.1"
                     /translation="MFRPRSSRPLILLVDDDPFQSEVASYIAEELDCDFEFALSGTEA
                     LAKVAQRAPDVVLLDVKMDDLSGYEVCRRIKTFPETADTQVLFVTARTDEEDLLKGFE
                     ALANDYLTKPFSARELKARTKNALRTKHLLDTIGARTRFLELQQEITEKLETEDAGAE
                     EKRAQLLVPILERIGQLFGADGVTLHTRRPGRPETRTAVSPAWPDGTPPSYLAVLALS
                     EDPRIEKAPRLPGEARDTAGDWLVVSAPLWVGSELLGTLRLHRRGVPPAPGDSTELEH
                     LVALAGHLARTLHRSDLIQELRSH"
     gene            23499..24926
                     /gene="cysS"
                     /locus_tag="PLP50_09075"
     CDS             23499..24926
                     /gene="cysS"
                     /locus_tag="PLP50_09075"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPA51743.1"
                     /translation="MSLRLFDTAARSLVDFEPLHPGRIGLYTCGPTVYNRVHIGNLRT
                     FVWEDVMCRAFRSLGFDVTQVMNLTDIDDKTIRGALAEGLPLREFTDRHIATFFEDLK
                     TLNVIPAAVYPRATDHVPEMVEIVKKLTERGHTYESDGSIWFRIASFPSYGKLSRVDL
                     SSMKRGARVADDEYEKEDVRDFAVWKGAKKGEPESALWETELGKGRPGWHLECSAMSM
                     KYLGETLDIHTGAVDNVFPHHENEVAQSEGATGKTFVRFWLHAEHLIVDGEKMAKSKG
                     NFYTLPDVLARGFSPRAVRYLLLSVPYRQKLNFTFDALRGAVSALERLESLEARLGER
                     AGTTEAAGASEAFLARLAEARAGVRAAWEDDLNSAAALGALFGLVKEANTGLEAGQLA
                     PADAAAARAFLREVDGVLGVLAPAEDASLEAEVEAKIAARQEARKRRDFKASDAIRDE
                     LAARGILLEDTPQGVRWRRRSVMAP"
BASE COUNT         4233 a         8562 c         8907 g         3261 t
ORIGIN      
        1 gccgcgacgc gacgaagatc ctcacggtcg ccctcgcgca cgtcgagtac gagaccgaga
       61 agcgccacta cgcccacatc gactgcccgg gccacgccga ctacatcaag aacatgatca
      121 ccggcgcggc gcagatggac ggcgcgatcc tcgtcgtcga ggccccgaag ggcccgatgc
      181 cgcagacgaa ggagcacgtc ctcctcgccc ggcaggtcaa cgtcccggcg atggtcgtct
      241 tcctcaacaa gtgcgacctc atggaggacc cggagctcct cgacctcgtc gagctcgagg
      301 tccgcgacct cctgaacaag tacgagttcc ccggcgacac gatcaccgtc gtccgcggct
      361 ccgccgtcaa ggcgctcaac gacccgaccg gccccgacgc ccagtgcatc tgggagctcg
      421 cgaaggccct cgacgagaac atcccggacc cggtccgtga gaccgacaag cccttcctca
      481 tgccgatcga ggacgtcttc tcgatctccg gccgcggcac cgtcgtcacc ggccgcgtcg
      541 agcgcggcgt cgtgaaggtc ggcgaggaga tcgagatcgt cggcttcaag gacacggtca
      601 agaaggtcgt caccggcgtc gagatgttca agaagctcct cgaccagggc caggcgggcg
      661 acaacatcgg cgtcctcctc cgtggcgtcg agcgtacgga cgtcgagcgc ggccaggtcc
      721 tctgcaagcc gggcacgatc aagccccaca cgaagttcat ggggaagatc tacgtcctgt
      781 cgaaggagga gggcggacgc cacacgccgt tcttcaacaa ctaccggccc cagttcttca
      841 tccgcacgac cgacgtgacc ggctcggcga agctccccga gggcgtcgag atggtcatgc
      901 cgggcgacaa cgtcgagctg gagatctccc tcatcgcccc gatcgccatc gagaaggggc
      961 tgaagttcgc gatccgcgag ggcggccgca ccgtcggcgc gggcaccgtc acggagatcc
     1021 tcgcctaaac gagcgtccgg tcgcggggag gggccttcgg gcaccctccc ccgcggccgg
     1081 tcctccgccc cctggggcgg taccacgaga aagacgatgg gcaacgacaa gatccgcatc
     1141 cgcctcaagg gctacgacta ccgaatcctc gaccagtcga cgctcgagat cgtcgacacc
     1201 gcgcgccgct cgggtgccaa ggtcgccggt ccgattccgc ttccgacggc catctctcgg
     1261 tggacggtga accgctcgcc gcacgtcgac aagaagtcgc gggagcagtt cgagatgcgg
     1321 acccacaagc gcctcctcga catcttcgag tggacgccgc agaccgtcga cgccctcacc
     1381 aagctcgagc tccccgccgg tgtggaagtc gagatccgca ccatggggaa atgaccccgg
     1441 agccgtaacg aggtttttga ccatgatcac agggattctg gggcgcaaga tcgggatgac
     1501 ccagctcttc gcagccgacg gcacggtcgt gccggtgacg gtggtggagg ccgggccgtg
     1561 cctggtcgta cagcgcaaga ccaagcagaa cgacggctac gacgcggtcc agctcgggct
     1621 cgtcgggccc cgtccgtcgc cccgccgcct cacgaaggcc gtccgcggcc acttcgagaa
     1681 ggccggcgtc cccccgaccc ggaccgtcgg tgaggtccgg tgcgaggaag gggacgcgtt
     1741 cgccccgggc gacaaggtcc tctgcgacat cttcaacgcc gccgagcacg tcgacgtcgt
     1801 cggcgtctcg aaaggcaagg ggtttcaggg ggtcatcaag cgtcacggct tcggtggcgg
     1861 cgtcgcgacc cacgggtcga tgttccaccg cctgccgggc tcgatcggcg cctcggcctt
     1921 cccctcccgg gtcatcgccg ggacccgctc gtcgggccgg atgggcggga agcaggtgac
     1981 gaccaagaac ctcgaggtcg tcaaggtcga cgccgagaac cacctgatct acctgcgggg
     2041 tgcggtcccc ggcgcgcgga acacggtcgt caagatcgtg aagaagcccg ccaagacgca
     2101 ggcggcgtga ggagagggac gatggccacg acgaagaaga cgtcccccgc ctccggcctg
     2161 accgtgccga tccgcaacct gaacgccgag accgtcggcg aggcgaacct ccccgaggag
     2221 atcttcgggg tgccgttccg ccgtcacgtg gtgtgggagg tcgtccgggc gatccgcgcg
     2281 cgcgagcacg cggggaccca caagacgaag gtccgctccg aggtctcggg cacgggcaag
     2341 aagcctttca agcagaagca caccggccgg gcgcggcagg gcggcggccg tccgccgatc
     2401 caccgccacg gcggcacggt gcacgggccc gtcccgcgct cctacgagct caaggtcaac
     2461 gtcgccacga agaaggcggc cctccgctgc gtcctctcgg agaagctgcg cgagaaccag
     2521 ctcgtcctcc tcgagagcct cgagctcccg acgcaccgga cgaaggacct cgccgccgcg
     2581 atgacgaagc tcggcgtgac cgggaaggcc ctcctcctcg accgccggga gaacgagaag
     2641 ctcgcgaagg cggcgcggaa caacccgctc ctcctcgtcg aggacgccct ggggctcgac
     2701 gcgtacgccg ccctggaggc gggaacggtg gtcctgacgg agcaggcgct caagacggtc
     2761 accgaggtgc tcggatgaag accgcccagt cgatcctgct caagcccatc atcaccgaga
     2821 agtcgacgac gctgcgcgag gcgcagaacg tcctgacgtt caaggtcgcc ccggacgcga
     2881 accggatcga gatccgccgc gccgtggagg tcctgttcga cgtcaaggtc gactccgtgc
     2941 gggtcctgaa gacgacgtcg aagatcaaga aggtcgggcg ctccatcggt cgcacgagcg
     3001 aggggcggaa ggcctacgtg aagctgaagg cgggcgagaa gctgccgccg atcttcgagg
     3061 gggtctgaca tgccggttcg ctcctacaag cccacgtccc ccggtctccg gcagcggacg
     3121 acgagcgact tcgcggagat cacgcggagc gcccccgaga agagcctgac ccgcgggaag
     3181 gtgaagacgg gcggccggaa caacctcggc cagctcacct cctggtggcg cggcggcggg
     3241 cacaagcgcc gctaccgcgt catcgacttc aagcgcgaca agttcggcat tccgggcaag
     3301 gtcgcctcgg tcgagtacga cccgaaccgc tccgcgcgca tcgcgctgat ccactacgcg
     3361 gacggcgaga agcgctacat cctggcgccc gccgggatca aggtcggcca gaccgtcgtc
     3421 tccgggcccc aggcggacat cctgaccggg aacgcccttc ccctgaaggc gatcccgctc
     3481 ggcacggtcg tccacaacgt ggagctcacg cccggcaagg ggggccagat ggtccgctcg
     3541 gccggcgccg gcgcccagct catggcgaag gagggcgacc acgctctgct ccgcatgccc
     3601 tcgggcgagc tccgccgggt ccgggtcctc tgctacgcga ccgtcggcca ggtcgggaac
     3661 ctcgagcacg agaacgtctc gatcggcaag gcgggccgga accgctggct cgggtggaag
     3721 ccgcacaacc gcggcgtcgc gatgaacccg gtcgaccacc cgatgggcgg cggcgagggc
     3781 aagacgtcgg gcggccggca cccgacctcc ccgtggggct ggaagaccaa ggggaagaag
     3841 acccgcaaca acaagcgtac cgacgcgctg atcgtcaagc gccgcacgaa gtaaggcgga
     3901 gcgaaggaaa ccctgatgtc gcgttcgaca aagaaaggcc ccttcgtgga cgggcacctc
     3961 ctcgagaaga tcgtcgccct caactcggcg ggggacaagc gcgtggtcaa gacctggtcg
     4021 cgccgctcga cgatcatgcc cgagatggtc ggccacaccg tcgccgtcca caacggcaag
     4081 aagttcatcc ccgtctacgt ctccgagaac atggtcggcc acaagctcgg cgagttctct
     4141 cccacccgca ccttcaaggg ccactcgggc cggaaggtcg agaagggcgc ctcgccggcg
     4201 ccggccgcca agtaacggag gaccgatgga agccgtcgcc cgcctccgcc actaccgcgg
     4261 ctccgcccag aaggtgcggc tcgtcgcgga cctcatccgg atgaagccgg tcggccgcgc
     4321 gctcgcgacc ctccgcgcga cgccgaaggg ctgctcgccc gacatcaaga agctcgtcga
     4381 gtccgccgtc gcgaacgccc agcagaacgg ctcgggcgtc gacgccgacg cgctcgtcgt
     4441 ctcgcgcgtc ctcgtggacg gcgcggggcg caagcccctt cagcggaacg cgatgctccg
     4501 ctcggtctcc aactacaaga agcgcttcgc ccggccccgc ttcatgagcg gcccgcaggg
     4561 gcggatgtgg ctcgtcaccc ggcccttctg ccacgtcacg gtcgtcctct cggacgaccc
     4621 ggcggtccgc cgcaaggcgg ggctgtccgc cgagccggcg cgcccggtcg cgaagaagaa
     4681 gggcggcaag gtcgccgccg cggtcgcgaa ggccgccgcg cggccgcagg ccccgaaggc
     4741 cgacgcgaag cccgcgaagg ccgagggcgc cccgaaggcc ccgaggacca ggaagagcgc
     4801 gaaggccgcg gagcccgccg gcggtcagga gtaggacgaa gatgggtcag aaggttcacc
     4861 cctacgggtt ccgcatcggc tacaaccgga cctggcactc gcgctggtac gcggagaagg
     4921 actacctccg cttcctccac gacgacctga agctccgggc cgagctcaag cgcgacctct
     4981 cgaacgcggc cgtgtccgag gtcgagatcg agcgcgggaa caagctcaag atcaacatca
     5041 tgaccgcccg cccgggcgtc atcatcggca agaagggcgc ggccgtcgac cagctccgcg
     5101 accgcctcca gcgccgcctc ggcaaggaca tccacgtcaa catcgtggag atccagcgcc
     5161 ccgagaccga cgcccagctc gtctccgagt cgatcgcgat gcagctcgag cgccgcgtcg
     5221 cgttccgccg agcgatgaag aagggcatgg aatcggcctt ccggttcggg gcgcagggga
     5281 tcaagatccg ctgctccggg cggctcaacg gcgccgagat cgcgcggtcc gagtggtacc
     5341 aggaggggcg tctgccgctc cacaccctca aggccgacat cgactacggc ttcgccgagg
     5401 cccgcaccac ctacgggaag atcggcgtga agtgctggat ctacaagggc gacctccacc
     5461 gcgcgaagtc ccggcgcaag gtcgcgtaag gaacgttcgc catgttgatg ccctccaagg
     5521 tcaagtatcg caagcagcag aaggggcgcc gtcgcggcac cgcgctgcgg ggcgggacgc
     5581 tctccttcgg cgagttcggc ctccaggccc tcgaggcggg atggctgacc gcccgccaga
     5641 tcgaggccgg ccgcgtcgcg atccagcgtc acgtcaagcg cggcgggaag ctctggatcc
     5701 gcgtcttccc cgacaagccg atcaccaaga agcccctcga gacccgaatg gggaagggca
     5761 agggcgcgcc ggaggcctgg gtctccgtca tcaagcccgc ccggatcctg ttcgagatgg
     5821 agggcgtcga cgagaagatc gctcgtgacg cgttccgcct cgccgccgac aagatcggga
     5881 tcaagacccg gttcgtgtcc cggtccttcg aggaggccgt gcgatgaagg ggacgaccgt
     5941 cgagacgatg aagggctggt ccgccgacga gcggggcgcc aagctccggg agtggacgca
     6001 ggcgctgttc aacctccgcc tccagaaggc caccggccag ctggagaacc cgatgaagat
     6061 ccgccagctg cggcgcgaca ttgcgcggct gaagacggcc gagaggcagg cccgatgacc
     6121 accgagacca ccaccgcagc ggcgaagccc gcagcccgca agagccgcaa ggtcggcacg
     6181 gtcgtctcca acaagatgga gaagaccgtc gtcgtcgagg tcacccgcct cgtcaagcac
     6241 ggccggtacg gcaagttcca gaagaagtcc gcccgcttcc tcgcggacga ccgcgtccac
     6301 gcctgcatcc ccggggacgt cgtcgagatc gaggagacgc ggccgctctc ggcgcgcaag
     6361 cgctggcgcg tccgcgccgt cgtgaccgcg gcgcggcggg aagggtagag gagagacgcc
     6421 gtgattcaga tggggacgat cctcgaggtc gcggacaact ccggcgccaa gcgcctctcc
     6481 tgcatccgcc tcaagggagg gtcggtcggg cagtacggac gcctgggcga cgtcatcacc
     6541 tgcgcggtga gggagtccgc cccggacgcg cccgacgcgg tcaagaaggg gaaggtcgtg
     6601 aaggccgtca tcgtgaggac gcggcgcgag cagcgccggc gggacggcag ctacatccgc
     6661 ttcgacaaca acgcggccgt cctgatcaac aacgacaagg aaccgatcgg gacccgcgtc
     6721 ttcgggccgg tcgctcggga gctccgcgag aagaagtaca tgaagatcat ctcgctcgct
     6781 cccgaggtga tctgaggcga gcgcgatgaa gaagaagcag cccgagtaca cggggaagac
     6841 cagggtccgg aagggcgacg aggtcgtcgt cctggccggc aaggaccggg gcgcgcacgg
     6901 gaaggtcctg cgcgtcgtcc ccgacaaggg acgggtcgtg gtggagggcg tgaacctcat
     6961 caagcgcgcc acgcgcccga acccgcagaa gaacatcaag ggcgggatcg tcgagaggga
     7021 ggcggccatc gccatctcga acgtcatgct ccgcgacccg gagacgggga agccgacccg
     7081 cgtcggcgtc cgcgtcgaag ggggggagcg cgtccgctac gcgcggcgga gcagcacgac
     7141 gatctcctcc gtgacgaaga agacgggagc gtaagcgatg tcgtcgcggc tcaacgaaaa
     7201 gtaccgcaag gacgtcgtcc cggtcctgaa gaaggagttc gggatcgaca acccgatggc
     7261 ggtcccgaag atcgagaagg tcgtcctgaa catgggcgtc ggagaggcga tcacgaacgt
     7321 gaagatcgtc gaggccgccg ccgaggagct gacgaagatc gccggccagc gcgccgtcat
     7381 cacgcgcgcc cggaagtcga tcgcgacctt caagctccgc gaaggcatgc cgatcggctg
     7441 ccgggtcacg ctgcgcggcg agcggatgta cgagttcctc gaccgccttc tcaacatcgc
     7501 ccttccgcgg gtgcgggact tccgtggcgt gcccacgcgg gccttcgacg gccgcgggaa
     7561 ctacacgctc ggcatccggg accacctcgt ctttccggag atcgatccgg gcaaggtgga
     7621 caagtcgaag ggcctcaacg tcacgatcgt gacgacggcc ggctccgacg acagggcgcg
     7681 cgtcctgctc cgcgagctgg gcatgccgtt cgggcgctag gaacaaggga gaaccgtcga
     7741 tggctcgtcg ctcctcaatc gtaaagacca cgcggaagcc gaagttcgcg atccggacgc
     7801 acaaccggtg caagatctgc ggccggccgc gctcctacat ccgcaagttc ggcacctgcc
     7861 gcatctgctt ccgcaacctc gcgctcggcg gctacgtccc gggcgtgacc aaggcgtcgt
     7921 ggtgaggagg ggcgcgtggc cgcactgacc gacccgatcg ccgacctcct gacccgcgtt
     7981 cgcaacgggc tcacctcccg ccacgagaag atcgacgtcc cgtcgtcgcg cctgaagatc
     8041 gagatcgccc gcatcctgaa ggacgagggc tacatcaaga acttcaaggt gctcgacgac
     8101 aagggcgccg gcctcctccg gctgtacctc aagtacgacg acgcggggaa ccccgtgatc
     8161 cacggcctca cgcgcgtctc gcgccccggc cgccggctct accgcgggaa ggacgagctc
     8221 ccgcgcgtcc tcggcggcct cggcatcgcc atcgtctcga cctcgcaggg gctcctcacc
     8281 ggccacgacg cgcgcaagcg cggcctgggc ggcgaggtcg tctgctcggt ctggtaggcc
     8341 cggagggaag acgatgtctc gagtgggaaa gaagccgatc accctcccca agggcgtcga
     8401 ggtcaaggtc gacgccgccg gggtccacgt caaggggccc aaggggaccg tctccacgcc
     8461 ggtcctcgag ggaatcacgg tcgccgtcaa ggacggtcgc gtcgacctcg tccgcgcgag
     8521 cgaggagaag cgggtccgcg cgttccacgg gctgaaccgg gcgctcctcc agaacgccgt
     8581 ggtcggcgtc gcggagggct acaagcgctc catcgacctc gtcgggatcg gctacaaggc
     8641 cgagaagaag gggaacgagg tcatcttcac gtgcggctac tcgcacacgg tccggttccc
     8701 ggagccggac ggcgtgacga tcgaagtggc gccggacgcg aaggccaagg acgtgacgca
     8761 catcgtcgtc acgggcgtcg acaagcagaa ggtcggcgag acggcgaccc ggatacgcgg
     8821 catcaagccg cccgatccct acaagctcaa gggcttccgc ttcgtcggcg aggtcctgaa
     8881 gaagaaggcc ggaaaggccg ccggcaagtg aggtctcgag gatgaacagg gctcaggcac
     8941 gggtcgaggc gcgcgagagg atccacaccc ggatccgccg gaaggtcgag ggttcgccgg
     9001 agcggccgcg gctggccatc cgcaggactc tgaagtacat ctacgcccag ctcgtcgacg
     9061 acgcgtccgg ccggacgctc gcccaggcga gctcccgcga ggaggcgatc ctcggcggga
     9121 agaagagcgc cgcgagcaag ggcgccgctg tctccgtcgg cgcgctcatc gcggagcggg
     9181 cgaaggagaa ggggatctcc gcggtcgtct tcgaccgggg cggtcacctc taccacggca
     9241 acgtcaaggc tctcgccgac tcggcgcgcg agaaggggtt gaagttttga gcagcacacc
     9301 gaaccgtccc ccccgcttcg gaaaccgtcc gggcggcccg ggccgcggcc ccggcggtcc
     9361 gaacgccgcc ccgccccgtc cgccgggcgc gctcggcgag ggccgcgcgg gcctcgacct
     9421 gatcgaccac gtggtgcaca tcaaccgcgt cacgaaggtc gtcaagggcg gcaagaactt
     9481 ctcgttctcc gcgctcgtca tcgtcggtga cggcaagggc cgcgtcggct acggcgcggg
     9541 caaggcgaag gaagtcccca cggccatcaa gaaggggatc gagatggcca agaagacgat
     9601 gatctccgtg accctgaagg ggacgacgat tccccacgcg gtcacgggcc actacggcgc
     9661 cggccgcgtc ttcctcaagc cggcggccga gggcacgggc gtcatcgccg gcggcgcggt
     9721 ccgggcgatc ctcgaggccg tcggggtcca gaacatcctg acgaagtcgc tcgggacgac
     9781 gaacccgcac aacgtcgtca aggcgacgtt cgacgccctc cggaacctcc acctggccgc
     9841 cacgatcctc aaggcgcgcg gccggacggc cggagcggcg gccgtcgaag ccgtcgcggc
     9901 gccggcggcc gaggccgtcg cggctccgga gatcccggcg gagacgaagt gatggccgag
     9961 aaagagaaga gctccaagaa ggacgtcgac gccctcctga cggaagtggc gtcggcggcc
    10021 ggcgtgccga agaagacggc gaaatcgacc ggcgccgctc ccgcggcggc gaggccggcg
    10081 gcctcggcgg cgaaggccgc ggcgccggag gccgggaaga agacggtccg gatccgccag
    10141 gtgaagtccg gcatctgcgc cccggtcgac cagaagctga ccctcaaggg cctcggcctc
    10201 cggcggatcc gccaggaggt cgtgaggccc gacacgcccg ccctccgcgg gcagatcctc
    10261 aaggtccgcc acctcgtcga ggtcgtgggc gagtagcgag agggacgaga caccatggct
    10321 cacggactcc acaacctcgg tccgcgcaag ggcgcgacgt cggcgcgcaa gcgcgtcggc
    10381 cgcggcccgg gctccggcct cggaaagacg tcgggcaagg gccacaaggg ccagcagtcg
    10441 cgcagcggct actccggcca gctcggccgc gagggcggcc agatgcctct ccaccgccgg
    10501 ctcccgaagc gcggcttcaa gaacatcttc cgcaaggagt ggaacaccgt cccggtcgag
    10561 gcgctcgagc gcctcttcgc ggcgggtgcc accgtcgacg ccgaggcgct cggcaaggcc
    10621 ggcctcctca agcgcaaggg cgccgaggtg aagatcctcg ggacgggcga cctgaagaag
    10681 acgcttatcg tcgtggcgca ccagttcacc gagggcgccc ggaagaagat cgaagcggcg
    10741 ggcggccgct gcgaggacgt cccggcgtga tcgtcctcga gtccttccgg aatatcttcg
    10801 cggtcccgga cctcaggaag agggtcctct tcaccttcgg gctgctggcg atctaccgga
    10861 tcggcgcgca catcccgacg ccggggctcg acgccctggc gctcgaggag ttcttcaagg
    10921 cgagcgccgg cgggctcctc gggttcctgg acatgttctc ggggggcgcc ctccgccgac
    10981 tctccgtctt cgcgctcggc atcacgccgt acatcacggc gtcgatcatc ctgcagctcc
    11041 tcaccgtcgt ctggccctac ctcgagaagc tctccaagga aggggagatg ggccggcgca
    11101 agatcacgca gtacacccgc tacggcacgg tgctcctctc ggtcgtccag ggcgcgggca
    11161 tcgccctctg gctctggggg acgacgacgc cgacgggcaa gttcctcgtc ctccgggacc
    11221 cgacgtccga gggcggcaag atcccgttcg tcctgatggc gatcctcacg ctgacgaccg
    11281 gcaccgcgtt cgtcatgtgg ctcggcgagc agatcaccga gcgcgggatc ggcaacggca
    11341 tctcgctcat catcttcgcg ggcatcgtcg tcgggctgcc caccgcggtc tggcactcgt
    11401 tccggcagat ccagcgcggc cagatgtcgc tcttcaccgc cctcctcctc ggcgtcttca
    11461 tgctcgccgt cgtcgcggtg atcgtcttcg tcgagagggc gcagcggcga atcccggttc
    11521 agtacgccaa gcgcgtcgtc gggcgccgcg tctacggcgg tcagaacacc tacctgcccc
    11581 tccgcgtgaa cacgggcggc gtcatcccga tcatcttcgc cgtctccatc atccagttcc
    11641 cgcagtacct cgcctccgcg ttcaagagcg acttcatgaa gaagctggcc gaccagctcg
    11701 ccatggggca gccgctctac aacctcttct acgccctggc gatcatcttc ttctgctact
    11761 tctacatctc gatcatcttc aacccgaccg acaccgccga gaacatgcgg aagtacggcg
    11821 ggttcatccc gggcatccgt ccgggcaagg cgacgtcgga ctacatcgac agcgtcctga
    11881 cgaggatcac cctcatcggc tcgctctacc tggtcgtcgt cgcgctcatc ccggagttcc
    11941 tgctgatggg cttcaaggtc cagagcatcc cgggcatcgg accgtggctc gacgcgaacc
    12001 tcccgacgtt catcacccag ggcctcggcg tgccgttcta cttcggcggg acgtcgctcc
    12061 tgatcgtcgt cggcgtcgcg atggacaccg tgcagcagat cgagtcgcag ctcgtgatgc
    12121 ggaactacga cgggttcctg aagaagggcc gcatccgggg gcggcggagc taggacgatg
    12181 ccggtcgacg tggtgttctt cggcccgccg ggctcgggaa aggggacgca ggcctcgcgc
    12241 ctgtcgtcca cgctcgggat cccccaggtg tccaccggag acctcctccg ctcgaacgtc
    12301 gcccggggca cggacctcgg gaagatcgcc cggccgatca tggagtcggg ggctctcgtc
    12361 cccgacgacc tcgtcacacg catgctgaag gagaggctcg aggagcccga cgcggcgacc
    12421 ggcgccctct tcgacggcta tccgcgcacc gtgccccagg cggaggagct cgaccggctg
    12481 ctccggatcg agggccgctc cctaacggcg gtgctcttca tcgacgtccc cgaccccgtc
    12541 atcgtcggca ggctcgtgaa gcgagccgag atcgaagggc gggcggacga cacgccggag
    12601 acggtcgcgg cgcgcctccg ggtctaccgc gacaagaccg agccgctcgc cgagctctat
    12661 cggaagctcg ggctcttcca ccggatcgac ggcgacaggt ccgtcgacga ggtggccctc
    12721 gacgtcctgg cgacgctgaa ccgcgtcacc gggagggtcc tggcgtgatc acctgcaagg
    12781 gccgggcgga gctcctgaag atggagcggg cgacgtggat cgtccagcag acgctggccg
    12841 attgcgtcgc cgcgtgccgg cccggcgtga cgaccgaaga gatcgaccgg atcgccgcgg
    12901 acggcatccg gaagaggggc gggaaggccg ccttcccggg ctaccgcggc tacccgaaga
    12961 ccgtctgctc ctcggtcaac gacgaggtcg tccacgggat cccgacgtcg aagcggaagc
    13021 tccgcgacgg ggacatcgtc gggattgacc tcggggtcat cgtggacggg tacttcggag
    13081 acgcggcgcg gacggcgatc gtgggcgagg tgcccgcgcc ggtcgcggag ctcgtccggc
    13141 agacgtggcg cgcgctcgtc gccggcgtct ctgcggtccg cgtcggcggc cggatcggcg
    13201 acatcggcgc ggcggtcgag gccgtcgcga acgagaaggg gttcggcgtc gtccgggagt
    13261 acgtcgggca cggcatcggg accgccctcc acgaggagcc ccaggtgccg aactacggac
    13321 cggcggggaa gggcgagcgg atccgcgagg ggatgaccct cgcgatcgag ccgatgttca
    13381 acctcgggac ggcccgggtc cggctcggac ccgacggctg gaccgtgagg accgccgacg
    13441 ggaagccgtc ggctcacttc gagcacacgg tggtggcgac cgccgacggg ccggtcgtcc
    13501 tcggcttcgg ccgctacacg tccgacggtc tcgtgacggg ggtccccgac gagacggatt
    13561 acccggtccc ggcggcttcg ccgcgggggg agccggccga gaccccgggc gagagccggg
    13621 tctgagagga gagggtttcg aggcgcagat gtcgaaggaa gacgccatcg aggtgaccgc
    13681 gacggtcatc gagcccctgc cgaacgcgat gttcaaggtg gagctcgaga acaagcacca
    13741 ggtcctggct cacatttccg ggaagatgcg gaagcacttc atccgcatcc tgcccgggga
    13801 caaggtcctc gtggagctct ctccgtacga cctgaaccgg ggtcgcatca tctaccggta
    13861 caagtagtaa actgtaaggc tccgccggct cccccgggag gccgggggcg aaacgatcga
    13921 gggatcacat gaaagtccgc gcgtccgtga agaagatctg ctccaagtgc aaggtgatcc
    13981 gcagggcggg agtcgtccgg gtcatctgcg agaacgccaa gcacaagcag cggcagggtt
    14041 agagggaaga gggaatggct cgaatctccg gcgtcgatct ccccccgaac aagcgtgtcg
    14101 agatcggtct cacctatatc ttcgggatcg ggcgccccac ggcccgcatg atcctgaagg
    14161 acacgggggt gggacccgac acgaggatca aggacctgtc cgaggacgag gtcgcgaaga
    14221 tccgcaagaa gatccaggac gacgtgaagg tcgagggcga cctccgcaag gagaccgccc
    14281 aggacatcaa gcgtctgatg gacatcggct gctaccgcgg gcttcgtcac cgccgcggcc
    14341 tgccggtccg tgggcagcgg acgcacacga acgcccgcac ccggaagggc ccgcgtcgcg
    14401 gcgccgtggc cggcaagaag aaggcgacga agaagtaagg atgaggaacg ccgatggctg
    14461 ccgctgaagc taagggccgg aagaagggcc ccaagaagga aaagaagaac gtcccgcacg
    14521 ccgtggccac ggtccaggcg tcgttcaaca acacgatcgt ggcgatcacc gacccctccg
    14581 ggaacgtcct cgcctggtcg tccgccggcc ggatcggctt caagggctcc cgaaagggga
    14641 cgccgttcgc cgcccagctc gcggcgggca acgcggccca gctcgcgcag gagcacggtg
    14701 tgcggacgct cgacgtccgc gtgcaggggc cgggcgcggg gcgcgagagc gcgatccgcg
    14761 cgctcgccgc ggccgggctc gacatccgct ccatcaagga cgcgaccccg atcccgcaca
    14821 acggctgccg cccccgcaag cggcgccgcg tctagtcccc atcccgatcc gggggagcgc
    14881 cgctcccccg gacgggtcgg cgagaaaggg aggcccgcgg gcctcccgac ccccctctga
    14941 gggggggcga ccccggcgcg cggtgagcgc gccggcgccc gtaaaccggc tcccgattcc
    15001 gggccgggac gaagggcgga acgcaacgac ggagtcggcc aggaggtttc cgttcccgtg
    15061 gcgcgttacc gcgaatccgt ctgccggctc tgccggcgcg aggccatgaa gctcttcctg
    15121 aagggcgacc gctgcttcac gaacaagtgc gccatcgagc gccgcaactt cccgcccggc
    15181 cagcacggga agcgtcgctc caagatcctc ggctacggca tccagctccg cgagaagcag
    15241 aagctgaaga ggttctacgg gctcctcgag acgcagttcc gcctcacgtt cgagaaggcc
    15301 gagcggatgc gcggcgtcac gggcgagaac ctcctctcgc tgctcgagcg ccgcctcgac
    15361 aacgtcgtcc accggctcgg cttcgccgcc tcccggcagc aggcccggca gctcgtccgt
    15421 cacggacacg tcgcggtgaa cggccggaag gtcgacgtcc cgagcttcgt cgtcggtccg
    15481 ggcgcggtcg tctccatcaa ggagaagagc cgccagaacc cgggcgtcgt ggcggcggtc
    15541 gactcggcca aggggcgcgg cgttccggag tggctcgagc tcgactccac cgagttcaag
    15601 gggaccgtca agtcgctccc gcggcgcgag gacgtctccc tccaggtcaa cgagaagctc
    15661 atcgtcgagc tctacagcaa gtgacgggcc ccggcccgag gagaagcgaa cgatgatgtg
    15721 gaagggcttc cagcgaccga accgcctcga ggtggacgag gagtcgggga actacggccg
    15781 cttcaccgcc cagccgctcg agcgcggctg gggaacgacg gtcggaaacg cgctccggcg
    15841 cgtcctcctc tcttcgatcg agggagcggc gatcaccgcc gtcaagatcg acggcgtcga
    15901 gcacgagttc acggcgatgc cgggcgtcgt cgaggacgtc acggacgtca tcctgaacct
    15961 caagagcctc gccatccgga tgcacgacga cgggccgcgg atgctcgtcc tcgacgcgaa
    16021 gtcgaagggc gtggtgacgg ccgacgcgtt cgaggacgac gcccaggtcg agatcctcaa
    16081 ccccgaggcg acggtcgcga cgctcgccga cggcggccgc ctccgcctcg aggcgacggt
    16141 cacgaagggg cgcggctacg tccccgccga tcgtaacttc gacgccgagg cgccgatcgg
    16201 gacgatcccg ctcgactccg cccactcgcc ggtccgccgc gtgaactacc acgtcgaggc
    16261 cgcccgcgtc ggccaggcga ccgactacga caagctcgtc ctcgaggtct ggacgaacgg
    16321 gacgctcacg ccgcgcgacg cggtcggcat cgccgccgtc ctcctgcgcg agcacctcgc
    16381 catcttcgtc aacctcgaga cggaggaagg cgcggccgcg agcggacccg agatcccgca
    16441 gagcgagctc gacgcccttc tcgaccagaa cgtcgaggag ctcgagctct cggtccgcag
    16501 cgcgaactgc ctgaagaacg ccggcatccg gacgcttcgg gagctcgtgc agaagaccga
    16561 gaaggagatg ctcgagacga agaacttcgg gcggaagagc ctgaacgaga tcaaggagat
    16621 cctgcgcgag aaggggctcg ccttcggcat gaagctggag accccgacgg gcgcgggacg
    16681 ctgaggacct gaccgatgca gcacaaccgg gccggacgca agctggggcg gaccagcgcg
    16741 caccggaaag cactcttccg caaccagctc gactccctca tccttcacga gcggatccag
    16801 acgacgatct cgaaggcgaa ggagctccgg ccgctcgccg agaagctcgt cacgctcgcg
    16861 cggcgcggcg gcctccacgc gcgccgcctc gccctccggg acatcagcca ggaagccgcc
    16921 aagcggctct tcgacgagat cgcgccgcgc ttcgcgacgc gtcccggggg ctacacccgg
    16981 atcctgaagc tcggccagcg gccgggcgac gctgcggaga aggcgatcat cgagttcgtc
    17041 gacttcgact acgcgcagcg ccgcgccgag aagaaggccg ccgagaaggc cgtcacggag
    17101 aagaaggagt cgctcctcga gaaggcgcgg cgcctcgtca ccggaaaggg cggcgagaag
    17161 gacggcgcga aggccgacga gggggccgag gcggcgaagg ccgacgagaa gaaggccgag
    17221 aagaaggccg ccgctccgaa ggccgccaag cccgcgaaga cggccgcgcc gaaggccccg
    17281 aagccgaaga ccgagaagaa ggggggctcg actccccgga cgcctcgcaa ggccggcggc
    17341 gcctagaccc gtgttccccg ggagccctcc gccgtcgggc ggagggctcc cgatcgccgc
    17401 gctccttcct gcggcgatcg accccggacc cgccatgcga gccttcctct tcgtttcact
    17461 gtggaccgtc gtgatcgtcg gagggctgcc gcggcggacg caggaggcgc ttcagtttcg
    17521 cttcggatgg tgggacgcga agctggcggg actcttcagc tccgctctcg agacgttcgc
    17581 gggataccgc ctcatgcgtg tcacgctggc ggcgttctcc gccggtcagg tgtcggtgac
    17641 ggcgaccgtc atcctgtgcg ccatcgccgc gttccttctc ctcgagggcg ggatccggct
    17701 cctcgtgacg taccgtctgg acgacctggc cgtcccgtcg ctccccgtct gggcggtcgc
    17761 gaggctcgtc gggcgcctgg gaccgaaggg ccggggggac tccccggagg cgtgaggtcc
    17821 ttcagcggcc gccgagcagc aggacgaccg cgacggcctc ccgcgtcccg tgcgtgatcg
    17881 agacgtgggc gccgacgacg ccgagcgcgt cggctctctc ccgggcccgg ccggtcagga
    17941 cgagccgggg cgggccgccg gagtctcgcg ccacggcgat ctcccggaac gccacgccct
    18001 gcatcccggt accgagcgcc ttcatcgcgg cctccttcgc cgcgaagagg ccggcgacgt
    18061 gctcgccgat gcgccgggaa cggagcgggc gacggacctc gcccgggacg cagaccctct
    18121 cgaggaaacg cgagccgtgg cgcgcgaggg cggcctcgat gcgcgcgatc tcgcagaggt
    18181 cgaccccgag gcccaggacg gcttcgccgt ggggatacgc gcccgcgacg agcgccgagc
    18241 gggcgtccgt cgtcacgcgg agccctccgc gcgcccgagg aggacgcgcg ccgccaggac
    18301 gaggtggagg accccgagac cggcgacgaa gccgcggaag gacggggccg cgaggagcgg
    18361 ttcgagggag acgggagacg ggaggacgac tcgcctgacc cagaacgtgc tccagggggc
    18421 gaggaggagc agcagcccgg cctccaggaa gtagaggccg aggaggagct gcaggaaccg
    18481 gccgctccgc ttctcgctca cgccggcatt ctagagggcg aacgagagcg ggcgccgccg
    18541 ggggcggcgc ccgcgtatcg ggccgtcgag cgggactcgt tcagccgcgc ggcgaggcgc
    18601 tgcggcgcga ggaatcgccc gagcgggagg gaggagggct cgacggacgg gacgacgagg
    18661 acgaagagga ggaacgggac ggggcgctcg tacggggcgc cggcgccgaa cggggcatcg
    18721 aggacgaacg cgaaggggga gcctcgtacc ggggagccgg ggcggaccgc tgcggggacg
    18781 acgggcggga gggcgcctcg tagctccgcg acggggcctc gtacgagcgg gacggcggcg
    18841 cctcgtatcg gggggccggc gccgagcggg aaggggacga ggagcgcgaa ggcgcctcgc
    18901 cgccgcggga aggggtctcg gaggtgcggg agggaggcgt gtcggaccgc ggggcgggcg
    18961 ccgagcgcgg cgtgtcgttc ccgcgccatc cctcgtccgc gcggggcgcc ggggcgctcc
    19021 tgcggggaga cggagcggcc tcggcgtcac gggtgcgggc gggcgtgtcg gaaccgccgg
    19081 aaggctcgct tcctcgccag gtctcggagc gaggcgtggt ctcgcgcgtg ccggtccgcg
    19141 ccggcgtctc cgaggactcg accgtgggcg cggcccgccg ggcggggacg tcgaacgtgc
    19201 gcgacggagt cgagccggag cgcccttcgc ttcccggcga gcgccagccg tcgtctccgc
    19261 ggacgtcgcg gtttccgaac cctccgcgaa gcgggggctc ggtgtcgttc cgggtggcac
    19321 cgctcgtctc gccccggctg ccggcatccc gccagccatc gcccgtccgg gtcgtggagc
    19381 cgcgcacggg gctcgcggcg tcggccgtcc gcgagggcgt cgacggggcg cccacgcccc
    19441 gctcgcttcc gcgggagggt accgtcgcgg tcgaggagcc ggccgaacga cggggagcgg
    19501 cgagctggtc ggcggggacc ggtcgatagg aggggtcctc tccggcggtc acgaaggccc
    19561 gccgaaggcc gtcggcctgg gtcgaaccga gggtcccctc gcgacggagg accggcgtga
    19621 ggtcgtcgag cccgcttccc aggcgggggc cggcgtcgag ggtcctgggg acgcggcgca
    19681 ccgcttcctg gacgacggtc gtcacggaac cgccgcggcc gcgctcgatt cgaaggggcg
    19741 tcgtggcgac gagcccacgc tcgccgggct ggaagcggac cgactcgtgt ccgaggacgt
    19801 ccctgcggga gtcgaagcgc gacccgagcc ggctgacgga cgtgtagctc cagccgcggg
    19861 ggtcgatccg ccccaggtcg atccgtccgc gcaggtgggg gtaggcgtgc gtccaacgct
    19921 ccgatccgcg ccgattccgg tgggaacggt cgtagtagga gtggtagtag ctgccgtagt
    19981 agccgatcgg gcaccagccg acccacgaag gggtgtagga ccagtagacc caggccgggg
    20041 agtagcggat gccgggggtc cagtaccagc cgaaaccgac gtcccagctc caggagccgt
    20101 agtggtaggg gagccagccc cagggctcgt aggagaccca ggtcaggccc acgggggacc
    20161 agcgccagta gccgtccgtg tagggccgcc agccgacggc gacgctgggt cgccaggccc
    20221 gggaaccgcc caccgtgatc caggatccgt aacggtcgaa gtcggcgacg gcgtagtcgt
    20281 actcgtagcc ggcggccacg tcgtccgaag ccgagcggtc gtcggagtag gcccgacggt
    20341 cggagacgaa ggcggcgaag cggtcgcgcg gctcgccgag ccggtcgacc tcgagctcgg
    20401 agtccccgga gacgaacgcg tactggccgg cccggagcgt ggctcgcccg gagcgcgcgg
    20461 cgacctcggc gcggccggtg aggacgtaga cctcggtgcc gcgccggccc gtttcgaccc
    20521 ggaggaggcc cttgtcgggg aagaggaccg tcacgtcggc ggtatcgatc cgggtcgctt
    20581 cgtcgcggga cgtcgaccag cggtgctcga ggtggacgcc gccgcgctcg aggtagatcg
    20641 agttccggtc gtcctcggac tcgtaggtcc gggcgagccg atcgaagcga agctcggtgc
    20701 cgacgtcgat ccgcaggatg ttcccgtcgg cgaggatcgc ctcggcgcgg gaggtcgagc
    20761 ccgtcgagat gcgatcgccg acgaggatcg gcgtgttcgg ccgagccgtc gagtcgtcct
    20821 cggcgctcga gacgagcgtg acctcggagc cgacccagga gaggtacgag tacccctcgc
    20881 gctgcgcgag ggccggaacg gccgtcagga ggaaggcgat tgcgagcgcg ggccgaagga
    20941 ttctggtggt catcggagtc atcctcccgt ctcgggagat gcgacccgca tgccaacgtc
    21001 gggagtcccc ccggggagac gatcgcgccg gcattgcggg cggccggcgg cgcggggagc
    21061 cccccggccg aaggacctgg ggaggtcccg cacggaagtg ccttgacccg gaggccttgc
    21121 cgcgccaccc ttgagacgtg gtcttcgaat tcctgaagaa gaagctccaa tcgctcacgt
    21181 cggacgctgt ctccgcgcct gagccccgac cgggggccgc agcggactac gaggagcgcc
    21241 gcgcgcgcgc cgaggccctg cacgagaagg gagaaacggc cgaggccgtc gccctcctgg
    21301 agggtctcgc cgcggatctc gccggcgagg ggagcttccc cctggccgtc gccgtccgtc
    21361 atcagctcag ctcctggggc gccggtcctc cggcgaccca gacagcggcc cgcgatgccg
    21421 aggagatggc ccggaagcgg gaggagaccg gcgtcgtccg tgcccggcaa accgggaagg
    21481 cgtcggcggc gatccccgaa gggctcgagc gctcgcgctt cttcgccgag atgacgaagg
    21541 aggagatcgc ggggttcatc gcgtcgacgg tgcggcggac cttccgcgaa ggcgagatcg
    21601 tcctcgagca ggggaagacc gggcgcgacc tctacatcgt cacccgcggc gtccttcgcg
    21661 tggaggccgc cgtcggcgag gggcggaagc tccggatggg ggtcctgatg gcgggcgacg
    21721 tcttcggaga ggtcgccttc ctgacggggc ggccgcgtac ggcgacggtc ctggcggaga
    21781 ccgccgcgga gtgtctcgag atcgggggcg aggcgtggga cggagtcctc gcggggctcc
    21841 cgcgggttcg gcaggtcctc gaagaggtcc accaggagag ggcccggttc gcggcggacg
    21901 tgatcgtcga ggagctgagg cggaagagag agaagggaag agagggctga atggcgatcc
    21961 gggacatcgt caggtacggc gacgagcgcc tcgtcgcgaa gaacgaggac gtgatcgatt
    22021 tcgaggaccc ggcgctccag cagctgatcc aggatctgtt cgacacggga tgggccgcgc
    22081 ccggcctcgg gatcgccgcg ccccagatcg gcgtgaacag gcgcgtctgc gtcgtcgacc
    22141 tctcggtcgg caaggaccct tcgcagcggc tcgtcctggt caacccgcgc gtcgtcgacg
    22201 ccgaggggct cgtgagggac gaggaggggt gcctctcgtt ccccgacatc gtcgagatcg
    22261 tcgagcgacc ggaggcggtc gcggtcgagg cgtacgacga gaagggggtc aagcgggtcc
    22321 tcgagggggc ggacctcctc gcccgggcgt tctgccacga gatcgaccac ctcgactcca
    22381 ggctcttcat cgagcgcatg tcgagcctga agcggggatt cgtcctccgc aaggtgctca
    22441 agaggcagaa gcgcggcgag tggtagccgc ggcctggcgc cgcggggagg ctcgtggcga
    22501 gcctccccga tctctcaatg actccggagc tcctggatga ggtcggaccg gtggagcgtc
    22561 cgcgcgaggt gccccgccag ggcgacgaga tgctcgagct cggtcgagtc gccgggagcg
    22621 ggcgggacgc cgcgacggtg gaggcggagc gtcccgagga gctccgagcc gacccagagc
    22681 ggggcggaga cgacgagcca gtctcccgcc gtgtcgcggg cctcacccgg cagacgcggg
    22741 gccttctcga tgcgagggtc ctcggaaagc gcgaggaccg cgaggtagga cgggggcgtg
    22801 ccgtccggcc aggcgggcga gacggcggtc cgggtctcgg gccgtccggg ccgccgcgtg
    22861 tggagcgtga cgccgtcggc cccgaagagc tggccgatcc tctcgaggat cgggacgagg
    22921 agctgggccc gcttctcctc ggctccggcg tcttccgtct ccagcttctc ggtgatctcc
    22981 tgctggagct ccaggaaccg cgtccgcgcg ccgatcgtgt cgagaaggtg cttcgtccgg
    23041 agggcgttct tcgtccgggc cttcagctcg cgcgccgaaa acggtttcgt gaggtagtcg
    23101 ttcgcgagcg cctcgaaccc cttgaggagg tcctcctcgt cggtccgggc ggtcacgaag
    23161 aggacctgcg tgtccgccgt ttccgggaag gtcttgatcc ggcggcagac ctcgtacccg
    23221 gagaggtcgt ccatcttcac gtcgaggagg acgacgtccg gggcgcgctg agccaccttc
    23281 gccagcgcct ccgtgcccga gagcgcgaac tcgaagtcgc agtcgagctc ttcggcgatg
    23341 taggaggcga cctccgactg aaagggatcg tcgtcgacga ggaggatgag cgggcgggat
    23401 gagcgaggac ggaacatcgg aacgcgcttt atccctcgcg cgggcggggt cgtcaaggag
    23461 attgcgatgg gccctctccg cgaagagaat ccggaaccgt gagcctgcgt cttttcgata
    23521 ctgcggcccg ttccctggtc gatttcgagc cactccaccc cggccggatc ggcctctaca
    23581 cctgcggccc gacggtctac aaccgggtcc acatcgggaa cctccggacg ttcgtctggg
    23641 aggacgtgat gtgccgggcg ttccggagcc tcggcttcga cgtgacccag gtgatgaacc
    23701 tgacggacat cgacgacaag acgatccgtg gcgcgctcgc cgaggggctc ccgctccggg
    23761 agttcaccga ccggcacatc gccaccttct tcgaggacct gaagacgctc aacgtgatcc
    23821 cggccgcggt ctacccgcgc gcgacggacc acgtccccga gatggtcgag atcgtgaaga
    23881 agctgaccga gcgcggccac acctacgaga gcgacggctc catctggttc cggatcgcct
    23941 cgttcccgtc gtacgggaag ctctcgcgcg tcgacctctc ctcgatgaag cgcggcgcgc
    24001 gcgtcgccga cgacgagtac gagaaggagg acgtccgcga cttcgccgtc tggaaggggg
    24061 cgaagaaggg agagcccgag tcggccctct gggagaccga gctcgggaag gggcggcccg
    24121 gctggcacct cgagtgctcg gcgatgtcga tgaagtacct cggcgagacg ctcgacatcc
    24181 acaccggggc cgtcgacaac gtcttcccgc accacgagaa cgaggtcgcc cagagcgagg
    24241 gggcgacggg gaaaacgttc gtccgcttct ggctccacgc cgaacacctc atcgtggacg
    24301 gcgagaagat ggcgaagtcg aaggggaact tctacacgct ccccgacgtc ctcgcccgcg
    24361 gcttctcgcc gcgcgccgtc cgctaccttc tcctctcggt gccgtaccgc cagaagctga
    24421 acttcacgtt cgacgccctg cggggcgccg tctcggccct cgagcgcctc gagtcgctcg
    24481 aggcgcggct cggcgagcgg gcgggcacca cggaagcggc cggggcctcc gaggccttcc
    24541 tcgcccgcct cgccgaggcc cgtgccggcg tgagggccgc ctgggaggat gacctcaaca
    24601 gcgccgcggc gctcggcgcc ctcttcgggc tcgtgaagga agcgaatacc ggcctcgagg
    24661 cggggcagct cgcccccgcc gacgcggccg cggcccgcgc cttccttcgc gaggtcgacg
    24721 gcgtcctcgg cgtcctggct cccgcggagg acgcttctct cgaggccgag gtcgaggcga
    24781 agatcgcggc ccggcaggag gcgcgcaagc gccgcgactt caaggcctcc gacgcgatcc
    24841 gagacgagct cgccgcccgc ggcatcctcc tcgaggacac cccgcagggg gtccgctggc
    24901 gccgaaggag tgtcatggct ccttagaaat gtcaccccct ggaactcgat agaaatgtcc
    24961 gtt
//
LOCUS       DAOONL010000048        24955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1874_length_24955_cov_15.248876, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000048 DAOONL010000000
VERSION     DAOONL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24955
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_24955_cov_15.248876"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..721
                     /locus_tag="PLP50_09080"
     CDS             320..721
                     /locus_tag="PLP50_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPA51744.1"
                     /translation="MKLTTRSEYALLALIHLARHEGDEYVPVAAIAEAQGVPHRFLEQ
                     LLLVLKRARLVKSQKGQRGGYRLARPPAKISLAEVIRLLDGALAPTESVSTYFYEPTP
                     VERERRLLRVFQEIRDLVAAKLEKTTLADVR"
     gene            792..1574
                     /locus_tag="PLP50_09085"
     CDS             792..1574
                     /locus_tag="PLP50_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA51745.1"
                     /translation="MLDSLTILGGRSRTGEPEPVGRLDLRVGEVLAVVGPTGSGKTTL
                     VNDLELFADGTTSTGRKVLVDGVPPPSRMRDEPSASPIALITQHTSFLSDLPVGEFLL
                     THARIRRSPTAAGRVVEETLAFANELTGEPIDARSRMTDLSGGQARALLIADATVVCD
                     TPVVLLDEVENAGIDRARALALLRRHRKAFVFVTHDPHVALLADARLVMKGGAMTALL
                     RADEADRRLAAVAGRMDFALARVREAIRAGERLAGSELEAFA"
     gene            1571..2293
                     /locus_tag="PLP50_09090"
     CDS             1571..2293
                     /locus_tag="PLP50_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPA51746.1"
                     /translation="MRLAVVAGPATTGKTAVLRQVVRRLLARGVAPAFLKIDVQYADE
                     DEALAAEFGIPTRKVYSGELCPDHCSVMVLGDAVRWAAGAGAEVLLVETAGLCLRCSP
                     YVDGALGIVVLEATSGMNLPRKVGPMLSLADVAVVTKIDRVSQAEREVFRARIEDAAP
                     SVLVREVNALHGIGIDPLLGTLLGGPQLVLGEDLEPRGLRLRGAPPVGTCTICVGKRD
                     VGWRSHFGVVRPLESATFYRGE"
     gene            2294..3469
                     /locus_tag="PLP50_09095"
     CDS             2294..3469
                     /locus_tag="PLP50_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPA51747.1"
                     /translation="MQAYLDDAASTRLDPRVLDEMLPLLSAEWGNPSSLHTRGRSARE
                     AVERARERTARLVGAEPSEVVFTASGTEADNLAILGTAAALEGTPWHLVTSAIEHPAV
                     LEPCRFLERRGVPVTWLPCGPDGRVAPEALRAALRPETRLVSVMAANNVTGVVQPVRE
                     LAAICTERGVPFHTDAVQAAGKVPLDVLADGVSLLSLSAHKIHGPKGAAALVVRKGTP
                     LAPLLFGGGQEGGLRPSTEDVPAIAGLGKAAEIAALEMRDEAVRQVALREALVERVLA
                     GIPGASLLGDRWRRLPGHAGFLFEGLEGAAPALLLALDEAGVAVSTGSACSARHADRP
                     SHVLEAMGFDPVRARGALRVSMGRFTTRPDVERLLTALSEAAARLRAGAGLARPLIR"
     gene            3480..4181
                     /locus_tag="PLP50_09100"
     CDS             3480..4181
                     /locus_tag="PLP50_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPA51748.1"
                     /translation="MLAAEPIPVPDLPGLDCGLCGVRTCAELAERLPNRPELLARCIH
                     LSPASARREETKGAPLPVRGPSLGPVSAPRGASGAWTDSLGREFDFYLEHFPEEPGPR
                     EVILPHNPLIGRELELAPGDLILGRPLGMSCGCPITHCGVVTDVDRRTGVVVWCVTGP
                     LGARAGGAKDIGYYSAEAYEGLVTEARTPIEIGRRYFFQPRLCMLQWRHSGLVNYVSQ
                     GRDGIQVRVEGLWIG"
     gene            4178..5530
                     /locus_tag="PLP50_09105"
     CDS             4178..5530
                     /locus_tag="PLP50_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPA51749.1"
                     /translation="MRHVVVAGGGASGALLASSLLRLARRPLRVTVVDPSPSLGRGRA
                     YADGPPEHLLNVRAAGMSALADDPSHFARWLSRRLPGLRAPDEAFAPRALYGDYLGEL
                     LEGARGAAAPDVELVHLRDVVVDAGVDGSSVRCALASGESLAADHLAVATGNAPSRPP
                     LDPPPGASWRWEDPLDAAAVDSLPVDAPVAVVGTGLTLVDLLVRLDVRGHRGSVYAVS
                     RHGLLPRAHPLAPRLAEPAPWSACPRTARGLAGAVRRRAELHPHEDGTRPVEALRAVT
                     DAVWAELAPAERRRFLRHLLPLWNVHRHRMPPESAGVVARWRRTGRLRILAGRAVEVV
                     HGSEGALHRLLVRPRGEERVVSFEAATLFDATGPGDPLGTPLARALLARGLASPHPTG
                     LGLDVEPSGLVRPAGAEPAGLLGALGPIARGRTYESTAMREIRVQAARLAGTWIGPLS
                     "
     gene            complement(5550..6617)
                     /gene="hydE"
                     /locus_tag="PLP50_09110"
     CDS             complement(5550..6617)
                     /gene="hydE"
                     /locus_tag="PLP50_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPA51750.1"
                     /translation="MDRDGILAWLRETDPSRLSRLYRRADAVRREHVGNEVHLRGLVE
                     VSNHCVRRCAYCGIAATNDGIERYRMTEEEVLACARQAVELGYGTLVLQAGEDYGLTR
                     AAVSSMVRAIKRETGLAVTLSLGERPDDDLVAWREAGADRYLLRFETSDAALYEAIHP
                     AIPGRPDRFAILGRLAVLGYEVGSGVLVGIPGQTWESLANDLLVFRQLDLEMIGVGPY
                     IPHPATPLGSGRFLVAPEGEQVLADEETTTKVVALTRLVRPDANIPSTTALATLDPSW
                     GRERGLLRGANVVMPNLTPPAYRQLYEIYPGKACVSETAEACGSCLPNRIRMIGRVPG
                     TGPGGRRRTQEPSPDLGDVVA"
     gene            complement(6617..7873)
                     /gene="hydF"
                     /locus_tag="PLP50_09115"
     CDS             complement(6617..7873)
                     /gene="hydF"
                     /locus_tag="PLP50_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPA51751.1"
                     /translation="MSSAPRGLRTHIGLFGRRNVGKSSVLNALTRQEVAIVSATPGTT
                     TDPVEKPMELLPLGPVVFIDTAGIDDEGVLGEQRVAKTRAALDRTDLALLVAEAPAWG
                     PFEEELLGEIRSRSIPVVVVLNKADSGPPPADLAERLGAAGVPAVATSIPSGAGLDEL
                     RQALLATAPADAVEGRRIVGDLVSPGDVVVLVTPIDAEAPKGRLILPQVQTLRDLLDS
                     GALAVVAREHELRGALAALVAPPRLVVTDSQAFRQVAAEVPPGVPLTSFSILFARFQG
                     DLAEMARGVRGVDRLKAGDRVLVAEACTHHPAGEDIGRVKIPAWLRARAGVPLEFETV
                     QGRDFPDDLSRYALVVHCGSCMGNRRQMLSRLLRGRQEGVPVTNYGLTIAYSLGILER
                     ALEPFPSALEAFRLAAPPPSLPEVTT"
     gene            complement(7884..9296)
                     /gene="hydG"
                     /locus_tag="PLP50_09120"
     CDS             complement(7884..9296)
                     /gene="hydG"
                     /locus_tag="PLP50_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPA51752.1"
                     /translation="MTHSALSPEPVVRIDDGGIWSDLESASRSDAGEVRALLAKSREL
                     KGLRGRDLAVLANVTDPALLDELFHTAREVKEAIYGRRIVLFAPLYISNLCANECTYC
                     AFRARNTALVRRALTQEEISREVKWLEEQGHKRILLVAGESYPREGFDYVLRSIETVY
                     ATSSGPGEIRRVNVNVAPLTDGEFRRLKEAKIGTYQLFQETYHRETYAEVHVAGKKRD
                     YDWRVTAMDRAMRAGIDDVGIGILFGLFDWRYELLALMQHVEHLERTFGVGPHTISVP
                     RIEPATGSEVADHPPHAVSEVDFRKVIAILRLAVPYTGLILSTRESAETRREVLGLGI
                     SQISAGSRTNPGGYEADPGDACAEQFSLGDHRSLDEVIRDVAAHGFVPSFCTACYRLG
                     RTGADFMDVAKPGEIKEHCNPNALATFQEYLLDYGSAETRRAGESRIAATLEEMEPAL
                     REKSRKMVEAVKSGRRDVFC"
     gene            9628..11004
                     /locus_tag="PLP50_09125"
     CDS             9628..11004
                     /locus_tag="PLP50_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51753.1"
                     /translation="MPHTLVIVGSLVVFVLVLAWLVPSGTYQTVEKAGRTVTVPGTYQ
                     QVEKVWLGPQWVVVAPIRGFLDGALIIAFLFAIGGAFGIVQAGGTIEFAIRKLTAALA
                     ARPFLEKLVIPVLMAVFSLAGSVFGMSEEVIPFVVLCIPLAISLGYDSIVGVAIPFLG
                     AAAGFGAAFFNPFTVGIAQGLVGLPLYSGLGYRVATWFVTTGVMIAWVMVYAARVKKR
                     PETSPVHDLDVARERSAPASGEAEPWTVRHALVLSLFVTTMGVLVWGILKKQWFIEEI
                     GALFLAMGLVLGVVAGLGGTRIATAFVAGAKDMVNVALIIACGRALLIIAREAKVLDT
                     ILFHGAGLISHLPGVVAAQVMFVVQCVINFFIHSGTAQAALTMPIMAPLADLVGLTRQ
                     TIVFAYQLCELINPILPTSAVTMGVLGMAKIPWERWARWFLPLMLTLMVLAFLLLVPP
                     VLMHWGPF"
     gene            11015..12553
                     /locus_tag="PLP50_09130"
     CDS             11015..12553
                     /locus_tag="PLP50_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPA51754.1"
                     /translation="MKALALLFAAALLGPGPVAASGPGAVRPGLANVALPEDHRTLTR
                     STSYEEMAAFLKTIDGKGSIAVSVEGKTAAGRNLFLVNARGSASPTFRILFYAQQHGD
                     EVSGKDALLYLLRDLARDPKLLPPEVDLWVMPMVNPDGAEAATRRNAAGADLNRDHVT
                     LFHPETEALHRVARRVRPHLAVDAHEFGRDQEEWTRKGWEKWPDITMDGLNNPHFDRE
                     RVAAAKRWVDASAAPLEKAGHRFLRYWVGGAPPDDEQRHSAPDTDGGLNGLGAYGGLS
                     FIIEAAVRHRSPNPQADLGKRVDAYLVLFRRFLDGGGRRAEDRAVVEQAWKRPLPPFL
                     PSDALWVNPEGTVTEFPVVDLATGKALRVPTANVMTDVAVKRSVPTPLGYAVEPAAAA
                     EIGALLKRHGLAYETLAAPRTVTAETCALLRVEEEFDDLYGRYGGRTIVKRGAAKTIE
                     LPAGSLWVPLEGPDALRAAILLEPTAMYGLWQYPRLKALVAWDGTIPVRRVVALAAPA
                     GSPR"
     gene            12550..13704
                     /gene="menC"
                     /locus_tag="PLP50_09135"
     CDS             12550..13704
                     /gene="menC"
                     /locus_tag="PLP50_09135"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPA51755.1"
                     /translation="MSGPAERASALPLEAERVPAFERVDLVHVSLPFVKPFSISTATW
                     TGKETFLVRVEGGGKIGWGECVADPDPFYAPETTTSARHVVAEFLLPLVEPGLTFVEL
                     DGRFGHVRGNRMAKASLENALVDLAAKRKGVPLHELLGGARKRIPSGISIGLQDSPAA
                     LVRVVEEAVARGYHRVKVKVKKGKDVDYVAAVRERFPDLPLMADANGDYRLEDAAHLA
                     RLDAFGLTMIEQPLSYADIFEHSLLEKELATPLCLDESIHDLADFRAAVELGACRVLN
                     LKEGRVGGLLESLRLLAFARERGIPVWSGGMDETGIGRAVNIHLQTVEGFTLPGDTSE
                     TSRYFREDLVTPSVVLDAEGFVEVPAGEGIGVDVIPERLEKYTLRMERLR"
     gene            13701..15377
                     /locus_tag="PLP50_09140"
     CDS             13701..15377
                     /locus_tag="PLP50_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA51756.1"
                     /translation="MRKTVLVPALTIAAAFGGVLAAPPAVAQPSPASAAPATSAADAA
                     RPRFVLPKAGEAIPAPLRAALDGVRAAALRAHVDFLAAPALGGRGLGSDGLEAALEYG
                     AAQLALAGVAPYGATDAQGARTYFQPVPMREIREPGGSVEIERRAGETTARRTFVHGV
                     DVVVPPQPARSVSGPLVFAGYGIRESAPARDDYRGLDVKGRVVVLREGLPPGEAWARK
                     DLVEKYAAGDTEERWAAKLETAKALGAVAVLAVEGNDWATRLLGTEKPATFAFRSMAE
                     GPSLEPLVFRCAPAVLEALLGAAPKAEEPSRALPASATLRATAVEKTGLSRNVVGILP
                     GSDPALKGEAVVLGAHADHLGRVDGVTHPGADDNASGVAALLEIARAFASSPVQPKRT
                     LVFALWTGEEEDKIGSGHWVRDPLWPLARTAVYLNFDMVAHPWKAEEIRDLVTAAKLP
                     GAAAFLAAARPDSFIEPGVASFAPDLGPLLARAGSAAELALHLDWTEGVNGGSDYRDF
                     ARARVPFVRFFGNFHPDYHRPGDTPASIDPAQVRRVTRLGLATAWLAADR"
     gene            15904..16728
                     /locus_tag="PLP50_09145"
     CDS             15904..16728
                     /locus_tag="PLP50_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPA51757.1"
                     /translation="MRVRLAALALLLASGSALAVRPDKAGCKDHPLFPTRMPNYSIAA
                     CETKEFTAWPFKLQRGKTRVVEGRYTFITYTVDDRKDDQSGLAVVRNYENALKKIGGT
                     VADSVPNWWVNGSVVVDGKEVWAEAERGNGKIWLRIVERQEMTQHVVADAASFRDALK
                     TTGHVAVEGIYFDTGKSVVKPESTPALQEVAKLLAADPALKLWVVGHTDSVSAIDGNM
                     KLSQARAEAVVAALTSTHGVAVARLRGYGVGPLAPVASNDAEPGRAKNRRVELVKQ"
     gene            complement(16756..18096)
                     /locus_tag="PLP50_09150"
     CDS             complement(16756..18096)
                     /locus_tag="PLP50_09150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPA51758.1"
                     /translation="MPLIRVLAAFVLVSGLASAEPPGWDQGRSVYERRIRPPEAPEKV
                     DWEARVTALLREEPGDLVVAAVGDLIFNERISHLKEPERKNLLRILQEADVAIGNLEF
                     SINDRPELQKVFYNFRAPREFAWELAATGINLVSLANNHALDFGPEGLLECLRALDLS
                     GIAHAGAGRTLAEAHEAGTKRPPGQKGRVALLSYMRFWTDRDRCSDPAGPCLATIDPA
                     TVLVARPDGTVESVEGPQERDVARMEEDVLLAKRRFGPVFVAYHVHDVSHARVFGIQD
                     TTPPNEEIPFRRAVDAGADAVLGSGPHVLRGIELRNGRPIFYSLSNFVYQYRTPKAIP
                     TDLPHQRDSEMPRPANVSVWDRRDSREVMESVVARMTYRGGALRKLELIPVTIDDEGP
                     LYGVPRLASTKRAEEIVALLQRLSAPYGTKIALKDWYGEVELSAVNDGAQAPSE"
     gene            complement(18216..19088)
                     /locus_tag="PLP50_09155"
     CDS             complement(18216..19088)
                     /locus_tag="PLP50_09155"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPA51759.1"
                     /translation="MVRRDSAPLVLLALLLAVPSIPARADGPAPRAGHLVLIGGGEKP
                     ADAMRKFVELAGGKDAPIVVIPTASGEPDAAEYYEKLFREELGCTDTVSLRIRSRADA
                     ARPDFVALARKARGVFFGGGDQIRITNALLGTPVGDAIAAAFAEGAVVGGTSAGTACQ
                     SERMITGEGDFTQVRTRSVELWQGLGFLPSDVVVDQHFLRRQRQNRLLSVVLEKPELL
                     GVGVDEETAIWVRPDGTFQVLGESGVMIFDAKGVSLSRRPRPTGQDLLGVRGLTLHLL
                     LPGESYDLRTRRPL"
     gene            complement(19177..21927)
                     /locus_tag="PLP50_09160"
     CDS             complement(19177..21927)
                     /locus_tag="PLP50_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA51760.1"
                     /translation="MPRPISVVTGAVLILLLGLSGIASAQGTVTGSVQGIVRDSEGGV
                     LPGATVTAVSGALVRGRMTTTTDARGGWRFPALPPGTYAIEAELQGFRKIRRDDVRVR
                     LGQAIAVDLVLALESVAAEAIVTAEAPLVSVVENRVSTSFGEDYISRQAVPRNYYQVI
                     TTAPGVNADTGTSGSAILAYGGTSESQNAFTLDGVNVADSGSGQHWVLPSIQWMEEIQ
                     IGGLGANAEYGGYTGGVINGVTKSGGNEFKGALEAYYQPDSFTSDNSPDTEDETFEFE
                     NYAVSLGGPVVKDRLWFFASAEYWHQETTPVGATATSDRKIPRFLGKLTYQVSEKNRV
                     FGMFEYDHVENERRGIDAYTFPEAARKQDGPGVSFSVNWESILNSSNFLNVKLTGYDG
                     NDDYLPYNGTDTPGHEDLYGHTGWSLVNAKMQDLSHRHLVTGDASWSLFKDGLFGKAD
                     SHSFKFGALYEKASTTDEWRRNGGFTYYDDSTLCPGDTEAEQIANYLRDPSCGWYERS
                     TGYGEYEVHAEHTGYTFYAQDSIRLSRVTINAGLRYTNYSAGWREGFGNPDVYEVDFV
                     DPRIGFSWDVRGDGRWAIKAHWGRYHSSMYTYLFDREVSGHASVPDGDCYWDGSAWGD
                     CSSSTPDEAPIGDVDHPYVDETLFTVEHQLGRDSSIGVDFIDRRFRSLMGLYNTNSDY
                     ERVTGIANPFGGGDLEVWTLNGDPVWSLTTDNPGYRDYDSLVLRYDKRHANGWQLRSS
                     VVWTDLKGNVLKNNGYAPELEDRNGLVNADGRMDSSFNEWEFKVSGAVDLPLGIVASG
                     QYTYLSGTYWTPYGDVRSYVDGNFYSGRNVFLTERGSEKLPARNILDLRLGWGMNVRG
                     PLRLELSLECFNVLNSDKPLTVDNYYGRYRSGSWSEYAAFGEALSIEAPRQFRAGLRL
                     IF"
     gene            complement(22041..22766)
                     /locus_tag="PLP50_09165"
     CDS             complement(22041..22766)
                     /locus_tag="PLP50_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPA51761.1"
                     /translation="MTPRCKIYNVENIMQARSGPERHRQLRQWVADRLRAEILEGRRR
                     PGDWLRQESVAREEGVSQTPVREALKQLVAEGLLEHVPYRGIRVVTLSVEDAEDLYAS
                     RAVLEPMAARHAAGSITADEVRELASLHERMLACEIPARLREYRDLNRRFHETIIAAS
                     RRPFLTRTLGHLWSAFPTMLWSNVPGIASTSLPEREDPDAAEHAEIVAALVAHDPDRA
                     AAAVKRHVEEAGRTLVAAMRAHP"
     gene            complement(22800..24797)
                     /locus_tag="PLP50_09170"
     CDS             complement(22800..24797)
                     /locus_tag="PLP50_09170"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HPA51762.1"
                     /translation="MSALVETVLSAGTPEERADAFVRLLRESPPPAPGAAGPRSRVGT
                     ILDALEASPEVRSSFLSSLSALFAEGDGTALFGDVGIPSDKGFFAELGSRVSARLIPS
                     PRDAHDLAEIAARLFRSEADVVAFRELPLSDLHRLVRLLETGLSREASASLSRAFADG
                     FRLLLARIHAQGLSRELRSRATPGSISASPFHRLLRSGDALLDAWLCGREVGPALQSF
                     NKDSGDCRKEVRAIQRRLEEAGVSVDVVFGLEVIDRCLTRTALMADVITLGPGPDRSR
                     ALHRLLARLVLNAHEDRSVLHLVRWNLHLLDRKIVDRTGKTGEHYVAATRPEYRHIWL
                     AAAGGGIVTVFTAAFKMLVHEWHLAAFPESFLYGLNYAISFLLMQAFGLILATKQPAM
                     TAAALASILRETTGDDREERIAGFFARLTSSQLAAALSNVLVVSAGAAAFVAAWSALA
                     GRPWVDEKTARETFLVLSPLDSGTVFFAALTGVLLWLGSVAGGWFENWSTYHRLPEGI
                     ARHPIGRLVGADRMRRAADALSRNASGWATNVALGFLLGFAPAIGRFFGIPFDVRHVT
                     LSTGQLGLAASSLGQGWFAEGAFLRGLAGIGVMFVLNLSVAFSLSLFNAARAYEMPPG
                     ELAGVLKHVLQRALRRPLDFVRPPRPTPREGPAQEPSPPTE"
BASE COUNT         3463 a         8766 c         9125 g         3601 t
ORIGIN      
        1 ccgtcttcac gacgacccac gccccgaccc aggcccgctc gaggtcgtcc tcgagatccg
       61 cggcggccgg gcctgcggtc gtgaggatgc acgcgagcgc ggcgacgcgc agagacgacc
      121 ttcccggttt cgtcctcatc tcggctcctt ccggtggcgg gatcgtagcc gagtgcgagc
      181 ggcgggagcc cgggtcgatc cccgaccacg gagaagggcg aaacgcggcc ggaccgggag
      241 caccggtcac ggaccccttg acgcgggtcg gggcatggtc gcagtattcc gacgggacta
      301 gtcgggtaag gatgacggat tgaagctgac gacccgaagc gagtacgccc tgctcgccct
      361 catccacctc gcgcggcacg agggcgacga gtacgttccc gtcgcggcga tcgccgaggc
      421 gcagggggtc ccgcaccgat tcctcgagca gctcctcctc gtcctgaagc gggcgcggct
      481 cgtgaagagc cagaaggggc agaggggcgg ctatcggctg gcccggcctc ccgcgaagat
      541 ctcgcttgcc gaagtgatcc gcctcctcga cggcgcgctc gcgccgaccg agtcggtgag
      601 cacctatttc tacgagccga cgccggtcga gcgcgaacgg aggctcctca gggtcttcca
      661 ggagatccgc gacctcgtcg cggcaaagct cgagaagacc acgctggcgg acgtgaggtg
      721 agacctccac gccggacaag tggctttcgc cagctatttt ccgactaaat caatcgggta
      781 aggagtctgt gatgctggac agcttgacga tcctcggcgg gcggagccgg acgggagagc
      841 cggagccggt cgggcgcctc gacctccggg tcggggaggt cctcgccgtc gtcgggccga
      901 cgggctcggg gaagacgacc ctcgtgaacg acctcgagct cttcgccgac gggacgacgt
      961 ccaccgggcg gaaggtcctc gtcgacggcg tccctccgcc gtcgcggatg cgcgacgagc
     1021 cttcggcgag cccgatcgcc ctcatcaccc agcacacctc tttcctctcc gacctccccg
     1081 tcggcgagtt ccttctgacg cacgcgcgca tccgccgctc gccgacggcg gcgggtcggg
     1141 tcgtcgagga gacgctcgcc ttcgcgaacg agctgacagg agagccgatc gacgcgcgga
     1201 gccggatgac ggacctctcc ggtggacagg cccgcgccct cctcatcgcc gacgcgaccg
     1261 tcgtctgcga cacgccggtc gtcctcctcg acgaggtcga gaacgctggc atcgaccgcg
     1321 cgcgcgccct cgcgctcctg cgccggcacc ggaaggcctt cgtgttcgtc acgcacgacc
     1381 cgcacgtcgc cctcctcgcc gacgcgcgcc tcgtgatgaa gggaggcgcg atgaccgcgc
     1441 ttctccgggc cgacgaggcc gatcgccggc tcgccgccgt cgcggggcgg atggacttcg
     1501 ccctcgcgcg ggtccgcgag gcgatccgcg cgggcgaacg gctcgccgga agcgagctgg
     1561 aggcgttcgc gtgaggctcg ccgtcgtcgc ggggcccgcc acgacgggaa agacggccgt
     1621 gcttcggcag gtcgtccgcc gcctcctcgc gcgcggcgtc gcacccgcgt tcctgaagat
     1681 cgacgtccag tacgccgacg aggacgaggc gctcgcggcc gagttcggca tcccgacgcg
     1741 gaaggtctac tccggggagc tttgcccgga ccactgctcg gtcatggtcc tcggcgacgc
     1801 cgtccgctgg gccgcagggg cgggagcgga ggtcctcctc gtcgagacgg ccgggctctg
     1861 cctgcgctgc tcgccgtacg tcgacggagc gctcgggatc gtcgtcctcg aggcgacgag
     1921 cggcatgaac ctcccgcgga aggtcggccc gatgctctcc ctcgccgacg tcgccgtcgt
     1981 gacgaagatc gaccgcgtct cccaggccga gagggaggtc ttccgcgccc gcatcgagga
     2041 cgccgcgccg tccgtcctcg tccgcgaggt gaacgccctc cacggcatcg gcatcgaccc
     2101 gctcctcggg acgctcctcg gcgggccgca gctcgtcctc ggggaggacc tcgagccccg
     2161 cggcctgcgg ctccgcggag cgccgcccgt cggcacctgc acgatctgcg tcggcaagcg
     2221 cgacgtcggc tggaggtccc acttcggcgt cgtccgcccg ctcgagagcg cgacgttcta
     2281 ccggggggag tgagtgcagg cgtacctcga cgacgcggcc tcgacccgcc tcgaccctcg
     2341 cgtcctcgac gagatgctcc cgctcctttc ggccgagtgg gggaacccct cgagcctcca
     2401 cacgcggggc cggtcggcgc gggaggcggt ggagcgggcg cgcgagagga cggcccggct
     2461 cgtcggcgcg gagccctcgg aggtcgtctt cacggccagc gggacggagg ccgacaacct
     2521 cgcgatcctc ggcacggcgg cggcgctcga ggggacgccc tggcacctcg tcacgagcgc
     2581 gatcgagcac ccggcggtcc tcgagccgtg ccgcttcctc gagcgacgcg gcgtcccggt
     2641 gacctggctg ccgtgcggcc cggacggccg cgtcgcgccc gaggccctcc gggccgccct
     2701 ccgtcccgag acgcggctcg tctcggtgat ggcggcgaac aacgtgacgg gcgtcgtcca
     2761 gccggtccgt gagctggccg cgatctgcac ggagcgcggg gtccccttcc acacggacgc
     2821 cgtccaggcc gcgggcaagg tgccgctgga cgtcctcgcg gacggcgtct ccctcctctc
     2881 cctctccgct cacaagatcc acggcccgaa gggggcggcg gcgctcgtcg tccggaaggg
     2941 gacgccgctc gcgccgctcc tcttcggggg tggccaggaa ggggggctcc ggccgtcgac
     3001 cgaggacgtc ccggccatcg cagggctcgg gaaggccgcg gagatcgcgg cgctcgagat
     3061 gcgggacgag gccgtgcggc aggtcgcgct tcgcgaggcg ctggtcgagc gcgtcctcgc
     3121 ggggatcccg ggcgcctcgc tcctcggcga ccggtggcgg aggctccccg ggcacgccgg
     3181 gttcctcttc gaaggcctcg agggagccgc gccggcgctc ctcctcgctc tcgacgaggc
     3241 cggcgtcgcc gtctcgacgg ggagcgcctg ctccgcgcgc cacgccgacc gcccctcgca
     3301 cgtcctcgag gcgatgggat tcgaccccgt ccgcgcccgc ggggcgctcc gcgtctcgat
     3361 gggccgtttc acgacgcgac ccgacgtgga gcgtctcctc accgccctct ccgaggccgc
     3421 cgcgcgcctc cgcgccgggg ccggcctcgc ccgtccgctg atccgctgag gaggagccga
     3481 tgctcgccgc cgaaccgatc cccgtccccg acctccccgg cctcgactgc ggcctctgcg
     3541 gcgtccgcac gtgcgcggag ctcgcggaac ggctgccgaa ccgtcccgag ctcctcgcgc
     3601 gctgcatcca cctctcgccc gcgtcggcgc ggcgggagga gacgaagggc gccccgctcc
     3661 cggtccgcgg cccgtccctc ggcccggtct cggcgccgcg cggcgcctcc ggggcgtgga
     3721 ccgactcgct cgggagggag ttcgacttct acctcgagca cttcccggag gagcccggcc
     3781 cgcgcgaggt gatccttccg cacaacccgc tcatcggccg cgagctcgag ctcgcgccgg
     3841 gagacctgat cctgggccgg ccgctcggga tgtcgtgcgg ctgcccgatc acccactgcg
     3901 gcgtcgtgac ggacgtcgac cggcggacgg gcgtcgtcgt ctggtgcgtc accgggccgc
     3961 tcggagcgcg cgcgggaggc gccaaggaca tcggctacta ctcggccgag gcctacgagg
     4021 gcctcgtcac ggaggcgcgc acgccgatcg agatcggccg ccgctacttc ttccagccgc
     4081 ggctctgcat gctgcagtgg cgccacagcg gcctcgtgaa ctacgtcagc cagggccgag
     4141 acggcatcca ggtgcgcgtc gaaggtctgt ggatcgggtg aggcacgtcg tcgtcgcggg
     4201 gggcggagcg agcggcgcgc tcctcgcctc gagcctcctc cgcctcgcgc ggcgcccgct
     4261 gcgggtgacc gtcgtcgacc cgtcgccgtc cctcggccgc ggccgcgcgt acgccgacgg
     4321 gcctccggag catctcctga acgtccgcgc cgcggggatg agcgccctcg ccgacgaccc
     4381 gagtcacttc gcgcgctggt tgtcgcggcg ccttcccggc ctgcgcgccc cggacgaggc
     4441 cttcgcgccc cgcgcgctct acggcgacta cctcggggag ctgctcgaag gggcccgcgg
     4501 cgccgccgcg cccgacgtcg agctcgtcca cctccgcgac gtcgtcgtcg acgccggggt
     4561 cgacggctcc agcgtccgct gcgccctcgc ctcgggggag tccctcgcgg ccgaccacct
     4621 cgccgtcgcg acggggaacg cgccgtcgcg gccgcccctc gacccgccgc ctggggcgtc
     4681 gtggcgctgg gaggatccgc tcgacgccgc cgcggtcgac tcgctccctg tcgacgcgcc
     4741 cgtcgccgtc gtcgggaccg gcctcaccct cgtcgacctc ctcgtgcgcc tcgacgtgcg
     4801 cgggcatcga gggagcgtct acgcggtgtc acgacacggc cttcttccgc gtgcccaccc
     4861 cctcgcaccg cggctcgccg agccggctcc ctggtcggcc tgcccgagga cggcgcgtgg
     4921 cctcgcaggc gcggtccgcc ggcgcgcgga gctccatcct cacgaggacg ggacgcgccc
     4981 cgtggaggcg ctccgggccg tgacagacgc cgtctgggcc gagctggccc cggccgaaag
     5041 gcggcgcttc ctgcgacatc tcctccctct ctggaacgtc catcgacacc ggatgccgcc
     5101 cgagtcggcg ggcgtcgtcg cgcgatggcg acggaccggc cgactccgca tcctcgcggg
     5161 ccgggccgta gaggtcgtgc acggctccga aggcgctctc catcgcctcc tcgtgcgccc
     5221 gcgcggagag gagcgggtcg tctccttcga ggccgcgacg ctcttcgacg cgacgggtcc
     5281 cggcgacccg ctcggaacgc ctctcgcgcg ggccctcctc gcgcgaggcc tcgcctcgcc
     5341 ccacccgacc ggactcgggc tcgacgtcga gccgtccggg ctcgtgcgcc ctgccggcgc
     5401 ggagccggcc ggacttctcg gcgcgctcgg cccgatcgca agagggcgca cgtacgagtc
     5461 gacggcgatg cgcgagattc gcgtccaggc ggcgcgcctc gcggggacgt ggatcggtcc
     5521 gctcagctga gggcgcctcc cgtcgtccgt caggcgacga cgtcgccgag gtcgggtgac
     5581 ggctcctgtg tcctcctccg tccccccggc cccgtccccg gcacccttcc gatcatccgg
     5641 atccggttgg gcaggcagct gccgcacgcc tcggccgtct ccgacacgca ggccttcccg
     5701 gggtagatct cgtagagctg ccggtaggcc ggcggcgtga ggttcggcat gacgacgttg
     5761 gcgccgcgaa ggaggccgcg ctcgcgcccc cacgaggggt cgagcgtcgc gagcgcggtc
     5821 gtggacggga tgttcgcgtc gggccggacc aggcgcgtca gcgcgacgac cttcgtggtc
     5881 gtctcctcgt cggcgaggac ctgctccccc tcgggagcga cgaggaagcg gcccgagccg
     5941 agcggcgtcg cggggtgcgg gatgtagggg ccgacgccga tcatctcgag gtcgagctgg
     6001 cggaagacga gcaggtcgtt cgcgaggctc tcccaggtct ggcccgggat gccgacgagg
     6061 acgccgctcc ccacctcgta gccgaggacg gcgagacgtc cgaggatcgc gaagcggtcg
     6121 ggacggcccg ggatcgccgg gtggatcgcc tcgtagaggg ccgcgtcgga ggtctcgaag
     6181 cggaggaggt atcggtcggc cccggcctcg cgccaggcga cgaggtcgtc gtccggccgc
     6241 tcgccgaggc tgagcgtcac ggcgagcccg gtctcccgct tgatcgcgcg gaccatcgag
     6301 gagacggccg cccgtgtcag gccgtagtcc tcgccggcct gcaggacgag cgtgccgtag
     6361 ccgagctcca cggcctgccg ggcgcaggcg aggacctcct cctccgtcat ccggtagcgc
     6421 tcgatgccgt cgttggtcgc ggcgatgccg cagtaggcgc agcggcggac gcagtggttc
     6481 gagacctcga cgaggccgcg caggtggacc tcgttcccga cgtgctcgcg ccggacggcg
     6541 tcggctcgcc ggtagaggcg cgagaggcgg gaggggtcgg tctcccggag ccaggccagg
     6601 attccgtcgc ggtcgatcac gtcgtcacct ccggaaggga cgggggcggg gcggcgaggc
     6661 ggaacgcctc gagggcggag gggaagggct cgagcgctct ctcgaggatg ccgagggagt
     6721 aggcgatcgt caggccgtag ttcgtcacgg gcaccccctc ctgccggccg cgcaggagcc
     6781 gggagagcat ctggcgtcgg ttccccatgc agctcccgca gtggacgacg agcgcgtagc
     6841 gggagaggtc gtccgggaag tcccgcccct ggaccgtctc gaactcgagc gggacgccgg
     6901 cgcgggcgcg gagccaggcg gggatcttca cgcggccgat gtcctcgccg gccgggtggt
     6961 gcgtgcaggc ctccgcgacg aggacccggt cgcccgcctt cagccggtcg acgccgcgga
     7021 cgccgcgggc catctcggcg aggtctccct ggaagcgggc gaagaggatg gagaaggacg
     7081 tcaacggaac gccggggggg acctccgcgg cgacctgccg gaacgcctgc gagtcggtga
     7141 cgacgaggcg cggcggggcg acgagggcgg cgagagcgcc gcgcagctcg tgctcgcggg
     7201 cgacgacggc cagggcgccc gagtcgagga ggtcccggag cgtctggacc tgcgggagga
     7261 tgaggcgtcc cttcggcgcc tcggcgtcga tcggcgtcac gaggacgacg acgtcgccgg
     7321 gggagacgag gtcgccgacg atccggcgcc cctcgacggc gtcggcgggg gcggttgcga
     7381 ggagcgcctg ccggagctcg tcgaggccgg cgccggaggg gatcgacgtg gcgacggccg
     7441 ggacgccggc cgccccgagc cgctccgcga ggtcggccgg gggcgggccc gagtcggcct
     7501 tgttgaggac gacgacgacc gggatcgagc ggctccggat ctcgccgagg agctcctcct
     7561 cgaacggccc ccacgcgggc gcctccgcga cgaggagcgc gaggtcggtc cgatcgagcg
     7621 cggcccgggt cttcgcgacc cgctgctcgc cgaggacgcc ctcgtcgtcg atcccggcgg
     7681 tgtcgatgaa gacgaccggc ccgagcggca ggagctccat cggcttctcg accgggtcgg
     7741 tcgtcgtgcc gggcgtggcc gagacgatgg cgacttcctg ccgcgtgagg gcgttcagga
     7801 cgctcgactt gccgacattg cgccggccga agagaccgat gtgcgtccgg aggccccggg
     7861 gggcggagct caagggccgg gcctcaacag aagacgtcgc ggcggccgct cttcaccgcc
     7921 tcgaccatct tccgggactt ctccctaagg gccggctcca tctcctcgag agtcgccgcg
     7981 atgcgggatt cccccgcgcg ccgcgtctcc gccgagccgt agtcgaggag gtactcctgg
     8041 aacgtcgcga gggcgttcgg gttgcagtgc tccttgatct cgcccggctt cgcgacgtcc
     8101 atgaagtcgg cgccggtccg gccgaggcgg tagcaggccg tgcagaacga cgggacgaag
     8161 ccgtgcgcgg cgacgtcgcg gatcacctcg tcgagcgagc ggtggtcgcc gagcgagaac
     8221 tgctccgcgc acgcgtcgcc cgggtcggcc tcgtagccgc cggggttcgt ccggctgccg
     8281 gccgagatct gggagatgcc gaggccgagg acctcgcgcc gcgtctcggc cgactcgcgc
     8341 gtcgagagga tcagtcccgt gtacgggacg gcgaggcgga ggatcgcgat gaccttccgg
     8401 aagtcgacct cggagacggc gtgcggcggg tggtccgcga cctccgaccc ggtggccggc
     8461 tcgatccgcg ggacggagat cgtgtgcggc ccgacgccga acgtccgctc gaggtgctcc
     8521 acgtgctgca tgagcgcgag gagctcgtag cgccagtcga agaggccgaa gaggatgccg
     8581 atgccgacgt cgtcgatccc ggccctcatg gcccggtcca tcgccgtgac gcgccagtcg
     8641 tagtcccgct tcttcccggc gacgtgcacc tcggcgtacg tctcgcggtg gtacgtctcc
     8701 tggaagagct ggtaggtccc gatcttcgcc tccttcaggc ggcggaactc cccgtcggtg
     8761 agcggcgcga cgttcacgtt cacgcggcgg atctcgcccg ggccgctgga ggtcgcgtag
     8821 accgtctcga tcgagcggag gacgtagtcg aacccctcgc gcgggtacga ctcgcccgcc
     8881 acgaggagga tccgcttgtg cccctgctcc tcgagccact tcacctcgcg ggagatctcc
     8941 tcctgcgtga gcgcccggcg gacgagcgcc gtgttccgcg cgcggaaggc gcagtaggtg
     9001 cactcgttcg cgcagaggtt cgagatgtag agcggcgcga agaggacgat gcgccggccg
     9061 tagatcgcct ccttcacctc gcgggccgtg tggaagagct cgtcgaggag cgccgggtcg
     9121 gtgacgttcg cgaggacggc gaggtcgcgg ccgcgcaggc ccttcagctc ccgcgacttc
     9181 gcgaggaggg cgcggacttc tccggcgtcg gaccgggagg cggattcgag atcgctccag
     9241 atcccgccgt cgtcgatccg gacgacgggc tcgggtgaga gggcggaatg cgtcatgggt
     9301 tctccctggc ggaccccgga tccggggtct cggccatcag ccatcatcgc gccccggacc
     9361 cctcccggtc cagcggagga tggcggcggc ggtgcccaat ggcgacgccg tcgcagagcc
     9421 gcgggggagc ccgggaacac cggaaatgtg cacgaaatgg accggtcgag ccccccggaa
     9481 ggacgcccgt tctctatgat gccggagcgc ccgcggagcg ctcgagaagc cgggagccgc
     9541 ccggggcgtc gtccacgaag gaacggaatc gggaagggag agggagcgat ggccgaggcc
     9601 gcgagcaaca gcggacgtac gttccgaatg ccccacactc tggtgatcgt cgggagcctc
     9661 gtcgtcttcg tcctggtcct ggcgtggctc gtcccgtcgg ggacgtacca gaccgtggag
     9721 aaggcggggc ggacggtcac ggtgccgggg acctaccagc aggtcgagaa ggtctggctc
     9781 ggcccgcagt gggtcgtcgt cgccccgatc cgcggcttcc tcgacggggc gctcatcatc
     9841 gcgttcctct tcgccatcgg cggcgccttc ggcatcgtcc aggcgggcgg gacgatcgag
     9901 ttcgcgatcc ggaagctgac ggcggccctc gcggcgcgcc cgttcctcga gaagctcgtc
     9961 atcccggtcc tcatggcggt cttctccctc gcgggctccg tcttcgggat gtccgaggag
    10021 gtcatcccgt tcgtcgtcct ctgcatcccg ctcgcgatct ccctcggtta cgactcgatc
    10081 gtcggtgtcg cgatcccgtt cctcggggcg gcagcgggct tcggggcggc cttcttcaac
    10141 ccgttcaccg tcggcatcgc gcaggggctc gtcggcctgc cgctctactc gggcctcggc
    10201 taccgggtcg cgacctggtt cgtgacgacg ggcgtcatga tcgcgtgggt gatggtctac
    10261 gcggcgcgcg tgaagaagcg cccggagacg agccccgtcc acgacctcga cgtcgcgcgc
    10321 gagcggagcg ccccggcgag cggcgaggcg gagccgtgga ccgtccggca cgccctcgtc
    10381 ctctcgctct tcgtgacgac gatgggcgtc ctcgtctggg ggatcctgaa gaagcagtgg
    10441 ttcatcgagg agatcggcgc ccttttcctc gcgatgggcc tcgtcctcgg cgtcgtcgcg
    10501 ggcctcggcg ggacgcggat cgcgacggcg ttcgtcgccg gcgcgaagga catggtcaac
    10561 gtggcgctca tcatcgcctg cggccgggcg ctcctcatca tcgcgcgcga ggcgaaggtc
    10621 ctcgacacga tcctcttcca cggcgcgggg ctgatctcgc acctccccgg cgtcgtcgcc
    10681 gcccaggtga tgttcgtcgt ccagtgcgtc atcaacttct tcatccactc ggggacggcg
    10741 caggccgcgc tcacgatgcc aatcatggcg ccgctcgccg acctcgtcgg cctgacgcgg
    10801 cagacgatcg tcttcgccta ccagctctgc gagctgatca acccaatcct cccgacctcc
    10861 gccgtcacga tgggggtcct cggcatggcg aagatcccgt gggagaggtg ggcgcgctgg
    10921 ttcctgccgc tcatgctgac gctcatggtc ctggcgttcc tcctcctcgt tccgcccgtc
    10981 ctcatgcact ggggaccgtt ctaggagcac ggccgtgaaa gcgctcgcgc tcctcttcgc
    11041 ggcggccctc ctcggccccg gtccggtcgc ggcgtccggg ccgggcgcag ttcgtcccgg
    11101 cctcgcgaac gtcgcccttc cggaggacca ccggactctg acgaggtcga cctcgtacga
    11161 ggagatggcg gccttcctga agacgatcga cgggaagggg tcgatcgccg tctccgtcga
    11221 ggggaagacg gcggcgggcc gaaacctctt cctcgtgaac gcgcgcggga gcgcgagccc
    11281 gacgttccgc atcctcttct acgcccagca gcacggcgac gaggtgtcgg ggaaggacgc
    11341 cctcctctac ctgctgcgcg acctcgcgcg cgacccgaag ctcctcccgc ccgaggtcga
    11401 cctctgggtg atgccgatgg tgaaccccga cggcgccgag gccgccacgc gacggaacgc
    11461 ggcgggtgcc gacctgaacc gcgaccacgt cacgctcttc cacccggaga cggaggcgct
    11521 ccaccgcgtg gcgcggcggg tgcggccgca cctcgccgtc gacgctcacg agttcggacg
    11581 cgaccaggag gagtggacga ggaaggggtg ggagaagtgg cccgacatca cgatggacgg
    11641 gctgaacaac ccccacttcg accgcgagcg cgtcgcggcg gcgaagcgct gggtcgacgc
    11701 gtcggccgcg cccctcgaga aggcgggaca ccgcttcctt cgctactggg tcggcggggc
    11761 gccccccgac gacgagcagc gccactcggc ccccgacacc gacggcggcc tgaacggcct
    11821 cggcgcctac ggcggcctgt cgttcatcat cgaggcggcc gtccgtcaca ggtcgccgaa
    11881 cccgcaggcc gacctcggca aacgggtgga tgcctacctc gtcctcttcc ggcggttcct
    11941 cgacgggggc ggccgaaggg cagaggaccg cgccgtcgtc gagcaggcgt ggaagcgccc
    12001 gctcccgccg ttcctgccgt cggacgcgct ctgggtgaac ccggagggga cggtgacgga
    12061 gttccccgtc gtcgacctcg cgacggggaa ggcgctccgg gtgccgacgg cgaacgtgat
    12121 gaccgacgtc gcggtgaagc ggagcgtccc gacgccgctc ggctacgccg tcgagccggc
    12181 ggcggccgcg gagatcggcg ctcttctgaa gcggcacggc ctcgcgtacg agacgctcgc
    12241 ggcaccgcgg acggtgacgg ccgagacgtg tgcgctcctc cgcgtggagg aggagttcga
    12301 cgacctctac ggccggtacg gcggccggac gatcgtgaag cgcggcgcgg cgaagacgat
    12361 cgagctgccg gccgggagcc tctgggtccc gctcgagggg ccggacgcgc tgcgcgccgc
    12421 gatccttctc gagccgacgg cgatgtacgg cctctggcag tacccccgcc tgaaggcgct
    12481 cgtcgcgtgg gacgggacga tccccgtccg gcgcgtggtc gccctcgcgg cgccggcagg
    12541 atcaccacgg tgagcgggcc cgcggaacgc gcgtcggcgc tgccgctcga ggccgagcgc
    12601 gtccccgcct tcgagcgggt cgacctcgtc cacgtgagcc tcccgttcgt gaagccgttc
    12661 tcgatctcca cggcgacctg gacggggaag gagacgttcc tcgtccgcgt cgagggcggc
    12721 gggaagatcg gctggggcga gtgcgtcgcc gatcccgacc cgttctacgc gccggagacg
    12781 acgacctccg ctcggcacgt cgtggccgag ttcctcctcc cgctcgtcga gccgggcctc
    12841 acgttcgtcg agctcgacgg gcgcttcggc cacgttcgcg ggaaccggat ggcgaaggcg
    12901 tcgctcgaga acgccctcgt ggacctcgcg gcgaagcgga agggcgtgcc gctccacgag
    12961 ctcctcggcg gggcgcgaaa gcgaatccct tcggggatca gcatcggcct gcaggactcg
    13021 cccgcggcgc tcgtccgcgt ggtcgaggag gcggtcgcgc gcggctacca ccgggtgaag
    13081 gtgaaggtga aaaaggggaa ggacgtcgac tacgtcgcgg cggtccgcga gcgcttcccc
    13141 gacctgccgc tgatggccga cgcgaacggc gactaccgcc tcgaggacgc cgcgcacctc
    13201 gcgaggctcg acgccttcgg cctcacgatg atcgagcagc cgctctcgta tgccgacatc
    13261 ttcgagcact cgctcctcga gaaggagctc gcgacgccgc tctgcctcga cgagtcgatc
    13321 cacgacctcg ccgacttccg cgccgccgtc gagctcggcg cctgccgggt cctgaacctg
    13381 aaggaagggc gcgttggcgg gctactcgag tcgctccggc tcctcgcctt cgcgagggag
    13441 cgggggatcc cggtctggtc gggcgggatg gacgagacgg gcatcggccg cgccgtgaac
    13501 atccacctcc aaacggtgga gggcttcacg cttccgggcg acacgtcgga gacgagccgg
    13561 tacttccgcg aggacctcgt gaccccctcc gtcgtcctcg acgcggaggg gttcgtcgag
    13621 gtgccggccg gcgaagggat cggcgtcgac gtgattccgg agcggctcga gaagtacacg
    13681 cttcggatgg agaggctgag atgaggaaga cggtgctcgt tcctgccctc acgatcgccg
    13741 cggcgttcgg cggcgtgctc gccgctccac cggctgtcgc gcagccgtct ccggcttccg
    13801 ctgctcctgc gacttcggcc gcggacgccg cgcggccccg gttcgtcctc ccgaaggcgg
    13861 gcgaggcgat tccggcgccg ctccgcgcgg cgctcgacgg cgttcgtgcc gcggcgctcc
    13921 gcgcgcacgt cgacttcctc gccgcgccgg ctctcggggg gcgtggcctc gggagcgacg
    13981 gcctcgaggc cgcgctcgaa tacggggcgg cgcagctggc cctcgccggc gtggcgccgt
    14041 acggcgccac cgacgcacag ggcgcacgca cgtacttcca gccggtgccg atgcgcgaga
    14101 tccgcgagcc ggggggctcc gtcgagatcg agcgccgggc cggggagacg acggcgcgac
    14161 ggacgttcgt tcacggcgtc gacgtcgtcg tcccgccgca gcccgcccgc tccgtctcgg
    14221 gaccgctcgt cttcgcgggc tacggcatcc gcgagagcgc gccggcgcgg gacgactaca
    14281 ggggcctcga cgtgaagggg agggtcgtcg tcctccgcga ggggctcccg ccgggagaag
    14341 cgtgggcgag gaaggatctg gtcgagaagt acgccgcggg cgacaccgag gagcgctggg
    14401 ccgcgaagct ggagacggcg aaggcgctcg gcgcggtggc cgtcctcgcc gtcgagggga
    14461 acgactgggc aacgcgcctc ctcgggaccg agaagcccgc gacgttcgcg ttccggtcga
    14521 tggcggaggg gccctcgctc gagccgctcg tcttccgctg cgcgcccgcc gtcctcgagg
    14581 cgctcctcgg cgccgcgccg aaggcggaag agccctcgcg cgcccttccc gcctccgcga
    14641 cgctccgcgc gacggcggtc gagaagacgg gcctctcgcg gaacgtcgtc gggatcctcc
    14701 cggggagcga tccggccctg aagggcgagg ccgtcgtcct cggcgcgcac gccgatcacc
    14761 tcgggcgcgt ggacggggtg actcaccccg gggccgacga caacgcctcg ggcgtcgcgg
    14821 cgctcctcga gatcgcgcgc gccttcgcgt cgtctcccgt ccagccgaag cggacgctcg
    14881 tcttcgcgct ctggaccggc gaagaggagg acaagatcgg ctcggggcac tgggtccggg
    14941 acccgctctg gccgctcgcg aggacggccg tctacctgaa cttcgacatg gtcgctcacc
    15001 cgtggaaggc ggaggagatc cgcgacctcg tcaccgcggc gaagctcccc ggcgcggcgg
    15061 cgttcctcgc cgccgcgcgc cccgattcgt tcatcgagcc cggggtggcg agcttcgcgc
    15121 ccgacctcgg gccgctcctc gcgcgggccg ggagcgcggc cgagctcgcc ctgcacctcg
    15181 actggaccga gggggtcaac ggcggcagcg actaccgcga cttcgcgcgg gcgcgcgtcc
    15241 ccttcgtccg gttcttcggc aacttccacc cggactacca ccgcccgggg gacacgcccg
    15301 cctcgatcga cccggctcag gttcgccgcg tcacccgcct cgggctcgcg accgcctggc
    15361 tcgccgcgga ccgctgaggg gcccgcccct cccgcggtcc gtcgcgctca cttccccttc
    15421 gagccgaaga gcctctgaag gaaggacggc tgcggagggc agctgcaacg ctgttccttc
    15481 gggacgttcg cgagggcttg ctcgacgtgg cgtccgcagc cggcccacgt ggggcggccg
    15541 cagcgggggc actggatctg gtggcacatg ggaggcggat tctgccacgc ccctgtggga
    15601 gcctcagcgg ctccgcgggc cgagcggccg cgggaggacc gactccggcg ggggacagga
    15661 gcagcgcctc tcgacgggga ctccggcgag cgcctcctcg acgtggcggc cgcaaccggc
    15721 ccaggcgggc ttcctgcact tcggacagcg aacgggatgg cacataacta tatgagtata
    15781 ttctagaaca aaacgggatg caaacggggc ttcgccgtgc cgccgccgtc gcttccggcc
    15841 ctagaattcc agatggcctt cgatgagaga cgtccgccga gctcggccga aagggatgca
    15901 cgaatgagag tccgcctcgc cgccctggcg ctcctcctcg cctccggctc cgccctcgcc
    15961 gtccggccgg acaaggcggg ctgcaaggac cacccgctct ttccgacccg gatgccgaac
    16021 tactccatcg cggcatgtga gacgaaggag ttcaccgcct ggcccttcaa gctccagagg
    16081 ggaaagacgc gcgtcgtgga gggcaggtac accttcatca cgtacaccgt cgacgaccga
    16141 aaggacgacc agtccggcct cgccgtggtc cggaactacg aaaacgccct gaagaagatc
    16201 ggcggcacgg tcgccgactc ggtgccgaac tggtgggtga acggctcggt cgtcgtcgac
    16261 ggcaaggagg tctgggccga agcggagcgc ggaaacggca agatctggct ccggatcgtc
    16321 gagaggcagg agatgacgca gcacgtcgtc gcggacgccg cctccttccg cgacgccctg
    16381 aagacgaccg ggcacgtggc cgtcgaaggg atctacttcg acacgggcaa gtccgtcgtc
    16441 aagcccgagt cgacaccggc gctccaggaa gtcgcgaagc tcctcgccgc agacccggcg
    16501 ctgaagctct gggtcgtcgg ccacaccgac tccgtcagcg cgatcgacgg aaacatgaag
    16561 ctgtcgcagg cgcgtgccga ggccgtcgtc gcggccctca cgtccaccca tggcgtcgcc
    16621 gtcgcccgcc tcaggggcta cggcgtcggc cccctcgccc ccgtcgcctc gaacgacgcc
    16681 gagcccggcc gtgcgaagaa ccggcgcgtc gagctcgtga agcagtagcg ccaccgccgc
    16741 cgggcccgcg gaggtttact ccgagggggc ctgcgcgccg tcgttcacgg ccgacagctc
    16801 tacctcgccg taccagtcct tcagcgcgat cttcgtgccg tacggcgcgg agagccgctg
    16861 gaggagcgcg acgatctcct ccgccctctt cgtcgacgcg agccgaggga ctccgtagag
    16921 cggtccctcg tcgtcgatcg tcacgggaat cagctcgagc ttccggagcg cgccgccgcg
    16981 gtacgtcatc cgcgcgacga cgctctccat gacctcacgc gagtcgcgcc ggtcccagac
    17041 cgacacgttc gcggggcgcg gcatctcgga gtcgcgctgg tgggggagat ccgtcgggat
    17101 cgccttcggc gtgcggtact ggtagacgaa gttgctcagg ctgtagaaga tcggtcgtcc
    17161 gttcctcagc tcgattccgc gcaggacgtg cgggccgctc ccgaggacgg cgtcggctcc
    17221 cgcgtcgacc gcgcgacgga acgggatctc ctcgttcggc ggggtcgtgt cctggatgcc
    17281 gaagacccgc gcgtggctca cgtcgtggac gtggtacgcg acgaagacgg ggccgaaacg
    17341 ccgcttcgcc aggaggacgt cctcctccat gcgcgccacg tcccgctcct gcggcccctc
    17401 caccgactcg accgtcccgt cgggtcgggc gacgaggacg gtcgccgggt cgatcgtcgc
    17461 gaggcacggt ccggccgggt ccgagcagcg gtcgcggtcg gtccagaagc gcatgtagga
    17521 gaggagcgcg acgcgcccct tctgcccggg gggccgcttc gtgccggcct cgtgcgcctc
    17581 tgcgagggtc cggccggccc cggcgtgggc gatccccgac aggtcgagag cgcgcaggca
    17641 ctcgaggagc ccctcggggc cgaagtcgag ggcgtggttg ttcgccaggc tcacgaggtt
    17701 gatcccggtg gcggcgagct cccacgcgaa ctcgcgcggc gcgcggaagt tgtagaagac
    17761 cttctgaagc tcgggccggt cgttgatcga gaactcgagg ttcccgatcg cgacgtcggc
    17821 ttcctgcagg atgcggagaa ggttcttccg ctccggctcc ttcaggtggc tgatccgctc
    17881 attgaagatc aggtcgccga cggcggcgac gacgaggtct cccggctcct cgcgcaggag
    17941 cgccgtcacc cgggcctccc agtcgacctt ctccggcgcc tccggcggcc gaatccgccg
    18001 ctcgtacacg gaccgcccct ggtcccaccc gggcggctcc gccgacgcca ggccggagac
    18061 gagaacgaac gccgcgagga cccgaatcag tggcacccac tgcggtgccc ccccacacgg
    18121 tgccacccca cgcggtgcca ggcgtgaaat catgtattgt tctcctcttt gcgaatactg
    18181 cacgatttca cgcctggcac cacctccggt acccgctaca ggggacggcg ggtgcggagg
    18241 tcgtaggact cgccaggcag gaggaggtgg agcgtcaggc cgcggacgcc gaggaggtcc
    18301 tggccggtcg ggcggggccg ccgggagagc gagaccccct tcgcgtcgaa gatcatgacg
    18361 ccgctttccc cgaggacctg gaaggtcccg tcggggcgaa cccagatggc cgtctcctcg
    18421 tcgacgccga ctccgaggag ctcgggtttc tcgaggacca ccgagaggag gcggttctgc
    18481 ctctgccggc ggaggaaatg ctggtcgacg acgacgtcgc tggggaggaa gccgaggccc
    18541 tgccagagct ccacgctgcg ggtcctcacc tgagtgaagt ctccttcgcc ggtgatcatc
    18601 cgctcgctct ggcaggccgt tccggccgac gtgccgccga cgacggcgcc ctcggcgaac
    18661 gccgcggcga tcgcgtcccc gaccggcgtt ccgagaaggg cgttcgtgat ccggatctgg
    18721 tctccgcccc cgaagaagac gccgcgggcc ttccgggcga gggcgacgaa gtcggggcgc
    18781 gcggcgtcgg cgcggctccg gatccggagc gagacggtgt cggtacagcc gagctcctcg
    18841 cggaagagct tctcgtagta ctccgcggcg tcgggctcgc cggacgccgt cgggatcacg
    18901 acgatcggcg cgtccttccc gccggccagc tcgacgaact tgcgcatcgc gtccgcgggc
    18961 ttttcgccgc cgccgatcag aacgagatgc cccgcgcgag gcgcgggtcc atcggcgcgc
    19021 gccgggatcg acggcacggc aaggaggagg gcgaggagaa cgagtggagc ggaatcacgg
    19081 cggaccatcg gctctcctgg gcgcccggcg gacgcggggc ggaagaaaaa gcgggggcgg
    19141 ccggcaggcc gcccccgatg agagaggagg agggacctag aagatcaggc ggagcccggc
    19201 ccggaactgg cgcggggcct cgatgctgag agcctcgccg aaggccgcgt actcggacca
    19261 gctgcccgag cggtagcggc cgtagtagtt gtcgacggtg aggggcttgt ccgagttgag
    19321 gacgttgaag cactcgaggg agagctcgag gcggagcggc ccgcggacgt tcatccccca
    19381 tccgagccgg aggtccagga tgttccgcgc cgggagcttc tcggatccgc gttcggtgag
    19441 gaagacgttc cggccgctgt agaagttgcc gtcgacgtaa gagcggacgt cgccgtaggg
    19501 cgtccagtag gtgccggaga ggtaggtgta ctggccgctc gcgacgatcc cgagcggaag
    19561 gtcgaccgcg ccggacacct tgaactccca ctcgttgaag gaggagtcca tccgcccgtc
    19621 ggcgttgacg aggccgttgc ggtcctcgag ctcgggcgcg tagccattgt tcttcaggac
    19681 gttccccttg aggtccgtcc agacgacgga ggagcggagc tgccagccgt tcgcgtgccg
    19741 cttgtcgtac cggaggacga gggagtcgta gtctcggtag ccggggttgt cggtcgtcag
    19801 tgaccagacg gggtcgccgt tcagcgtcca gacctcgagg tcgccgcctc cgaagggatt
    19861 cgcgatgccg gtgacccgct cgtagtcgct gttcgtgttg tagaggccca tgaggctgcg
    19921 gaagcgacgg tcgatgaagt cgacgccgat cgacgagtct cggccgagct ggtgctcgac
    19981 ggtgaagagg gtctcgtcga cgtacgggtg gtcgacgtcg ccgatcggcg cctcgtcggg
    20041 cgtggaggac gagcagtcgc cccaggccga gccgtcccag tagcagtcgc cgtcaggaac
    20101 cgaggcgtgg ccggagacct cgcggtcgaa gaggtaggtg tacatcgagg agtggtagcg
    20161 gccccagtgc gctttgatcg cccagcgtcc gtcgccgcgg acgtcccagg agaatccgat
    20221 ccgcgggtcg acgaagtcga cctcgtagac gtcggggttc ccgaaaccct cgcgccagcc
    20281 ggcgctgtag ttcgtgtagc ggaggcctgc gttgatcgtc acgcggctca ggcggatgga
    20341 gtcctgagcg tagaacgtgt agccggtgtg ctcggcgtgg acctcgtatt cgccgtagcc
    20401 ggtcgagcgc tcgtaccagc cgcagctcgg gtcgcggagg tagttcgcga tctgctccgc
    20461 ctcggtgtcg ccggggcaga gagtcgagtc gtcgtagtag gtgaagccgc cgtttcgccg
    20521 ccactcgtcg gtcgtggagg ccttctcgta gagggcaccg aacttgaagg aatgcgagtc
    20581 ggccttgccg aagaggccgt ccttgaagag gctccacgag gcgtcgcccg tcacgaggtg
    20641 ccgatgggag agatcctgca tcttcgcgtt cacgaggctc caaccggtgt ggccgtagag
    20701 gtcctcgtgg cctggcgtgt cggtgccgtt gtaggggagg tagtcatcgt tcccgtcgta
    20761 gcccgtcagc ttcacgttga ggaagttgga cgagttgagg atggactccc agttcacgga
    20821 gaaggagacg cccggcccgt cctgcttgcg ggccgcctcg gggaaggtgt aggcgtcgat
    20881 tccgcgccgt tcgttctcga cgtggtcgta ctcgaacatt ccgaacacgc ggttcttttc
    20941 gctcacctgg tatgtcagct tcccgaggaa tcgggggatc ttccgatcgg aggtggcggt
    21001 ggcgccgacc ggggtcgtct cctggtgcca gtactcggcg ctcgcgaaga accagagccg
    21061 gtctttcacg accggcccgc cgaggctgac ggcgtagttc tcgaactcga aggtttcgtc
    21121 ctcggtgtcg ggcgagttgt ccgaggtgaa agagtcgggc tggtagtacg cctcgagggc
    21181 ccccttgaac tcgttgccgc ccgatttcgt gacgccgttg atgacgccgc ccgtgtagcc
    21241 gccgtactcg gcgtttgcgc cgaggccgcc gatctgaatc tcctccatcc actggatgct
    21301 cggcagaacc cagtgctggc ctgagcccga atcggccacg ttcacgccgt ccagcgtgaa
    21361 ggcgttctgg ctctcactcg tcccgccgta ggcgaggatc gcgctgccgc tcgttccggt
    21421 gtcggcgttc acgcccgggg ccgtcgtgat cacctggtag tagttgcgcg ggacggcctg
    21481 ccggctgatg tagtcctcgc cgaagctcgt cgagaccctg ttctcgacaa cgctcacgag
    21541 cggtgcctcc gccgtgacga tggcctcggc ggcgacgctc tcgagcgcga ggacgaggtc
    21601 gacggcgatc gcctgcccga ggcggacccg cacgtcgtca cggcggatct tccggaagcc
    21661 ctgcagctcg gcctcgatcg cgtaggttcc cggcgggagc gcggggaagc gccagccgcc
    21721 gcgtgcgtcg gtcgtggtcg tcatgcgacc gcgaacgaga gcgcccgaga cggcggtgac
    21781 ggtggcgccg ggaaggacgc ccccttcgga gtcgcggacg attccctgga cgctgccagt
    21841 gacggtgccc tgggcgctcg cgatgcccga gaggcccaac agcaggatca gaacggctcc
    21901 tgtgacgacg gagatcggac gtggcatgac tcctcctctt gcccggcctc gggcttgttt
    21961 cgagccccgc gggacgagtg ttcgcttgcg atcgttcgag ccccgacgcg gggagagaaa
    22021 ctggatcccc gtcgggcgca tcacggatgg gccctcatgg cggcgacgag cgtccgtccc
    22081 gcctcctcga cgtgccgctt cacggcggcg gcggcgcgat cggggtcgtg cgcgacgagg
    22141 gccgcgacga tctccgcgtg ctcggcggcg tcggggtcct cgcgctcggg gagcgaggtc
    22201 gaggcgattc cgggtacgtt gctccagagc atcgtcggga aggccgacca gaggtggccg
    22261 agcgttcgcg tgaggaacgg tcggcgggag gccgcgatga tcgtctcgtg aaagcgccgg
    22321 ttcaggtcgc ggtactcgcg gaggcgggcc gggatctcgc aggccagcat tcgctcgtgg
    22381 agcgaggcga gctcccggac ctcgtcggcc gtgatcgagc cggccgcgtg ccgcgccgcc
    22441 atcggctcga ggacggcgcg gctcgcgtag aggtcctcgg cgtcctcgac cgagagcgtc
    22501 acgacgcgga tgccccggta ggggacatgc tcgagcagcc cctcggcgac gagctgcttg
    22561 agcgcctccc gaaccggggt ctggctgacg ccctcttcgc gggcgacgct ctcctgacgg
    22621 agccagtctc cgggcctgcg acgcccctcg aggatctcgg cccgaagcct gtcggcgacc
    22681 cactggcgaa gctgccggtg tcgttcgggt cctgatcgcg cctgcatgat gttctctaca
    22741 ttatatattt tgcatcgagg tgtcactcat ttttatccta ctgatcagaa aagacttagt
    22801 tactccgtcg ggggcgaagg ctcttgcgcc ggcccttccc ggggggtcgg ccgcggcggc
    22861 cgcacgaagt ccagcgggcg gcgaagcgct cgctgaagga cgtgcttcag gacgccagca
    22921 agctccccgg gcggcatctc gtaggcccgc gccgcgttga agagcgacag cgagaaagcc
    22981 accgagaggt tcaggacgaa catcacgccg atccccgcga ggccgcgcag gaacgcgccc
    23041 tccgcgaacc agccctggcc gaggctcgag gccgcgagcc cgagctgccc cgtggagagg
    23101 gtgacgtggc ggacgtcgaa cgggatgccg aagaagcggc cgatcgcggg agcgaacccg
    23161 agcaggaagc cgagcgcgac gttcgtcgcc cagcccgaag cgttgcgcga gagggcgtcg
    23221 gcggcgcgcc gcatccggtc ggctccgacg agtcgtccga tcgggtggcg ggcgattcct
    23281 tcggggaggc ggtggtaggt cgaccagttc tcgaaccagc cgccggccac gctcccgagc
    23341 cagaggagga ctcccgtgag cgcggcgaag aagaccgtac cgctgtcgag cgggctgagg
    23401 acgaggaacg tctcgcgcgc cgtcttctcg tcgacccacg gccggccggc gagcgcgctc
    23461 caggccgcca cgaaggcggc cgcgcccgcg ctcacgacga ggacgttcga gagcgcggcg
    23521 gcgagctgcg agctcgtgag ccgggcgaag aagcccgcga tccgctcctc ccggtcgtcg
    23581 cccgtcgtct cgcgaaggat gctcgccagg gcggccgccg tcatcgcggg ctgcttcgtc
    23641 gcgaggatga ggccgaacgc ctgcatcagg aggaagctga tcgcgtagtt gaggccgtag
    23701 aggaagctct ccggaaacgc ggcgaggtgc cactcgtgga cgagcatctt gaacgccgcc
    23761 gtgaagacgg tgacgattcc gccgccggcg gccgcgagcc agatgtggcg gtactcgggc
    23821 cgggtcgcgg cgacgtagtg ctccccggtc ttcccggtcc tgtcgacgat cttccggtcg
    23881 aggaggtgga ggttccacct cacgaggtgg aggacgctcc ggtcctcgtg cgcgttgagc
    23941 acgaggcggg cgaggagcct gtggagggcg cgcgaacgat ccggcccggg cccgagcgtg
    24001 atcacgtccg ccatgagagc ggtgcgggtc aggcaccggt cgatcacttc gaggccgaag
    24061 acgacgtcga cgctgacgcc ggcctcctcg agccgccgct ggatggcgcg cacctccttg
    24121 cggcagtcgc ccgagtcctt gttgaaggac tgaagggccg ggccgacctc ccggccgcag
    24181 agccaggcgt cgaggagagc gtccccggag cgaaggagac ggtggaaggg ggacgccgag
    24241 atcgagcccg gggtcgcgcg cgagcgaagc tcccgcgaga gcccctgcgc gtggatccgc
    24301 gccaggagga gacggaaccc gtcggcgaag gctctcgaga gggaggcgga ggcctcacgc
    24361 gagagccccg tctcgagcag gcggacgagg cgatggaggt cggaaagcgg aagctcccgg
    24421 aaggcgacga cgtcggcctc ggagcggaag agccgggccg cgatctcggc gaggtcgtgc
    24481 gcatcacggg gagaggggat cagccgggcg gagacgcggc tcccgagctc ggcgaagaac
    24541 cccttgtcgc tcggaatgcc gacgtcgccg aagagcgcgg tcccgtcccc ctcggcgaag
    24601 agcgccgaga gcgacgacag gaagctcgac cggacctcgg gggacgcctc gagggcgtcg
    24661 aggatcgtac cgacccggga cctcgggccg gccgcgccgg gcgccggcgg cggcgactcc
    24721 cgcaggagcc ggacgaaggc gtcggcgcgc tcctcgggcg tgcctgcgga gaggaccgtc
    24781 tcgacgaggg cgctcacgac gagcagaagg atagcgtctc ccgagcgccc ggtccggaag
    24841 cataatttag ccgtgcccgc gaacctgacg ccgccctacc acgacgccga ggagcggttt
    24901 aaggcggcgg cgacgcacga ggagaggctc gcggcgatgc gcgagatgat cgcgc
//
LOCUS       DAOONL010000049        24657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1904_length_24657_cov_19.517722, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000049 DAOONL010000000
VERSION     DAOONL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_24657_cov_19.517722"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLP50_09175"
     CDS             <1..570
                     /locus_tag="PLP50_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPA51763.1"
                     /translation="KYLCGGPGAPAFLWVPKRHQDVSLQPLSGWLGHAEPFAFESAYR
                     PAEGIARFLCGTPPVLSMSALDAALDVLLEADLALVRAKSESLTSFFLEAVEQACGGA
                     LRLVSPRDAARRGSQVSFAHPDAYAVMRALVARGVVGDFRAPDVLRFGFAPLYVRFVD
                     AWDAARALADVLSSHAHERPEFRRREAVT"
     gene            complement(572..988)
                     /locus_tag="PLP50_09180"
     CDS             complement(572..988)
                     /locus_tag="PLP50_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51764.1"
                     /translation="MKNADRNILLAATAALALAVAPSALADVRIHVNLPLPPPPHEVL
                     RHAPLPPPPHVDIRVDRRDRRDRRDRWRDADRRHRDRYRDDGWRNDHRYRDRDRWAFV
                     EGRWVRKPFLHAVWVNGYYDRWGRWVPGYWTRARYR"
     gene            complement(1182..2747)
                     /gene="ahpF"
                     /locus_tag="PLP50_09185"
     CDS             complement(1182..2747)
                     /gene="ahpF"
                     /locus_tag="PLP50_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HPA51765.1"
                     /translation="MLDPALAAQLQQIYSDLPVTVVLRQRPSEHPKNGELTTMLADVA
                     GTAPGKIVHLVEGEPSAIPAFEVAAEGRAATILFRGIPGGHEFSSLVLAVKYAAGQGK
                     RPDEGIQNRIRHLKGPVRLRTYVSLSCHNCPEVVQALNLMAVIHPDFVHEMVDGGLAE
                     GEVEKLGIQGVPTVISLVHENAQVSVGRAGLAELLDALEERFGSAPSEAPGAFPERDP
                     YDVLVVGGGPAGASAAIYSVRKGLRTALVAKNVGGQVLETLGIENLIGTTRTEGKRLA
                     ADLLSHLRSYPVEVLDNRRVEAIEDGDLKTVRLAGGEVLRTKALVMATGAQWRELNVP
                     GEKEYIGRGVAFCPHCDGPFYKDREVVVIGGGNSGIEAAIDLAGICSRVTVVEFMAEL
                     KADKVLVDNLKKLPNTEVIVNHRTAEILGDGSKVTAIKVVDRATDAERTFPIDGIFIQ
                     IGLAPNSGLVRGLVDVNRFGEIVVDEKCRTSTKGIYAAGDVTTVPWKQIVIAMGEGAK
                     AALAAFEDRIHAA"
     gene            complement(2878..3441)
                     /gene="ahpC"
                     /locus_tag="PLP50_09190"
     CDS             complement(2878..3441)
                     /gene="ahpC"
                     /locus_tag="PLP50_09190"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HPA51766.1"
                     /translation="MPVINSQIPEFKVQAYHNGAFKEVSNKDLAGKWSVFFFYPADFT
                     FVCPTELGDLADNYAEFKKMGVEIYSVSTDTHFTHKAWHDASDTIKKIQYVMLADPTH
                     ILSKGFGVHIDEAGLAYRGTFVVNPEGQVKIAEIHDNGIGRDAKELLRKVQAAQYVAS
                     HPNEVCPAKWTPGQETLKPSLDLVGKI"
     gene            3661..3737
                     /locus_tag="PLP50_09195"
     tRNA            3661..3737
                     /locus_tag="PLP50_09195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3695..3697,aa:Met,seq:cat)
     gene            complement(3819..6230)
                     /locus_tag="PLP50_09200"
     CDS             complement(3819..6230)
                     /locus_tag="PLP50_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPA51767.1"
                     /translation="MDARALLRRLPLRRALLWGTAGLGLLVAGPGVWRAGFVSPEPTL
                     LLRDRNDLFLGEVTLDEGQSQLGYWRLPAVPPRVAAATIAVEDRRFAWHPGVDLAAIA
                     RALTQNVRHARRVSGGSTLAMQVARLQHPGRRGLIRKAQEAVTAFLLTVRYGRQGVLR
                     HYLEIVPYGNRVHGIAYAARRYLDKPVEDLSWAETAFLTAIPQSPRRMNPFDPAGRLR
                     AVARGKRILRLLRRRGELTDAELALAAREIASLRVPPRRGRPPEAIHALLRFAEEARS
                     PGGREALAGRTVVKLTVDLPLQRRVALAARDSVTIWEKQGAGNAAVIVVDRKRNAIVA
                     RVGSTRWADAEHAGAIDYARVPRSPGSALKPFLYALALERGTITPATILPDLDRGAGG
                     ITNADDIFLGPLLPRVALANSRNVPAADLLDRVGLAEGYAFLRDLGLHDGSIPVHRYG
                     LGLAIGGLPVTLEGMVRAYSVLTNDGTLVEPLFWEGQPLPRPRRLLSEESARAVTLYL
                     ADPMARLPSFPRMGVTEYPFPVAVKTGTSSRYRDAWTAAFSTRYLVAVWVGHPDFKPM
                     NRLSGYRAAAELAQKVMLSLHDGETDGLGDLSFPAPRGSVSVRLCALTGRLATDACER
                     TVTEWIRPGQEPLDECAAHVKLAVDARSGLLATRATPRRFVEVRTFVDLGPQYAAWSA
                     AAGLPRPPGLVARRETSGGAFTSGAPPLVAGTTARVRITSPANGLRVLRDPETPADNA
                     TLALKAVVSPAAREIVWWVDGEPYATAPYPYTVRWKVTPGEHTFVARLPFVRLASPGV
                     TVRVE"
     gene            complement(6231..11978)
                     /locus_tag="PLP50_09205"
     CDS             complement(6231..11978)
                     /locus_tag="PLP50_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPA51768.1"
                     /translation="MKRGTVPIALLSLALSTVGVKASAAGSRANSAEPAARVVPDRLL
                     RRWDPVTVFFSADTGPKAGGPEDDAGRFATIDPAQPGAFRWLDAKTLQFRPAEPWPAL
                     SRFRVTPAGSKATTLATLLDAPASTVPPDGAEGLEPFDEITLTFAEPLPPAALAKMIA
                     LELRPLPGTSGGRRVPPDEIRIKPLERMSRTDAASYALSLAHAVPAGTRAVLTLKLSA
                     DEAAAGAARELSFSTGEPFRVVSFGCRERQLPVTPAGTLYEKDQALRCGSEAPAVLVE
                     FSSSPRPISPVEARNLVRFTPAVDDLKFETSARTLVATGKFRRDTLYAVTLLSTKMSD
                     ARGRALEPAGRSELFVVFPQRASFVKWGAGQGVVERLGAQMVPVEGRGAEKLDVRVQK
                     IDPLERSLWPFPDRGVSVDESERPPGPGEEPKAWDAPDQNIPPDGIEPHLRLLGSPPV
                     STIVTLPLRKDGGAARFGFDLTPHLAKISGPGEPGTYLVGLRPLEGGSLRSWMRVQAT
                     DLSLSTLEEPYAVRFAVTSLSTALPVAGATVRLEGTHRRPNDAKWETFAEGTTGPDGT
                     FHWKAPGRPDLRESRTMRRIVVTKGKDVLVVDAAHPPEKYADNQWSRSRETWLDWAFE
                     VLEGRGPMAETLCHLFTERPVYRPEEEVHLKGYLRERVAGRLHPLPAGEWTLVVSGPG
                     DMTWRFPVALSEGGSFYRLFNEKNLPTGVYDAHLEDKGQRKFGHVSFRMEAYRLPEFE
                     VDLHGPDRTALDKAFEVSLTATYYAGGRVSGRPVQWRVTQFPTDWQPPAREGFRFSSD
                     GRFSRTERFRSSPQTTKEGVTNDAGGATLKLDPTIEPTAQPRTYVVEATVTGADDQTV
                     TSTRRVAALPPFVLGLDVPRFVERAKALSPRVIAVGPDGKTLEGKAITVRLLKRQWHS
                     VLRASDFSDGVARYLTDVVDEKVSEQTVKSGKEPLTVPLPIETSGVYVVELEARDRLG
                     RAQVISVDLYAGGDEPMTWARPATKVFSVAPDKVKYQPGAKAAIVLQSPFQRARALAV
                     VEAPEGNRYSWIDVEKGAATFPLDILPTFVPRVPVHFVLMRGRIPGTGPAQGNATDLG
                     KPETMAATAWLQVEPTANRVEVKLAHPEVSMPGRTVDVTITLKDPAGKPLSGEVTLWL
                     VDQAVLALGKEQRLDPLPDFITPVRSHLVARDTRNSVFGLLPFAEAPGGDEGEEGGVL
                     DRATVRKNFKTVPFYDPAIVVGPEGTRTVQVPLPDNLTNFKVRAKAMSGPDRFGYGTG
                     TIAVRLPVLVQPAFPRFLRAGDVFTGVAVARIVEGKGGPASAQVKVTGAMLDGPATRS
                     LTMEPNKPERIEFGVKVPANGTGPITFQAGVERASDKASDAFEVKLPILPDRDPVARR
                     VVADLAPGAEFKVPEIQEPVRAGTLKRSLLLSSEPAVVKMASGLDFFDAYPYGCTEQK
                     VSAARALAAARKLRGVLGRTGDEDRASRAFATAQQAIADAVDANGLVAYWPGSTGYVA
                     LTAWSVDFLVEAKEASFATDARLLDRLLDALEKALRSDYAHFVGGESYLERTWALSAL
                     AHAGRFDAAYAAELARKPQVLRLEGKAQVLLAFSRSPDAKSEVSRGMANALWEGVVFR
                     SREGKPVYGGLQDGDRLGDLLLLCSEDRTLAEVLRALQRVAPEDPRIAALVNGLVVRG
                     RGDGWGTTNANAEALLALSDVLKSGRFEPFTCTVKEGASSRPLASASGSAVAKTVSTA
                     PAEAAIAVSGASRPLVVMAETRYVAAEDGSRAPAASGGFVVSREVAVVKADGPMEKTA
                     LAEPGKTIPLAVGTVVEDHVQVVNPEKRNHVAITVPLAAGMEPLNPALATAPPEATPS
                     KPPTLAPTYVKFLDDSVSWFYDSLPAGTYDFRFRTRATTPGSFVQPPAKCELMYDGAV
                     RGSSNGARIEVSRPQ"
     gene            complement(11975..17092)
                     /locus_tag="PLP50_09210"
     CDS             complement(11975..17092)
                     /locus_tag="PLP50_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51769.1"
                     /translation="MIRRALLLSFLAPQLLAADDVRVAPAAVPAAGRQTAVVTVPRFG
                     RYSLAVASKSGVALTVVDRMSGPGERNGTPGETDGRADVFLDRGEVRLVLNGDPKAKG
                     RATLSARPFVEGNGATPLRLVEGKPVETTLGDVEQRSWWISVEARRYVAFEAAGRRLT
                     DLRLWRDGSWLHAAEPESEVTMPQPGRPLRVIRLAADLAPGLYLLSAYGGPGEPWAEE
                     SDASPLHLRWGLPRVGIAGRARHVVGPSGIDRYLVGGGATYFNLEVPEARAISFSASD
                     WTESRPFVEGGERGAIDKKTNPPEAAIRTGGRTDGLTLVTVRGEAGQPYVLTHFELRD
                     RYPIRGSGDYWVGTIPSSDPRDAVDQTGLLLDDVSRTPIRTAAAEIDLGKPWAGRGNL
                     DGELTLFFQVGSAGRYVIDAAGVDAKIRVLPALGEGTPELKPFELRGRGTVDLDPGYW
                     QLTAQPVKKGIVTLSLHAEKEKAPAPAAPRGGILFPSVALSDRHGYTLVVGTRPRVVS
                     GLVLRSLPMDLTEPVPVAVRPGQTISVPFEATEEQTLIAETEDGGALPISVDGAPPVP
                     RPRVAKGRHEARVTLGGDAPRVVSLAARPRAFEKDVPLPPLPDGALAALPKFPALLEG
                     VAQGLDLAKDETATFNVTVPQPALYRIETTGLLATGALLRTRTVTQFRSEAENGVGRN
                     ALVADFLREGDYQVTVQPRGLSAGHLGVGLTKTRVVDGGAVTDGVPARLSLDAGEAAS
                     LVLDVKRAGSYRIRARGFKRTFAVRLEDAEGWPLLKPGIDGDLRQTLPKGTYRLVVLP
                     TAVPARVVVSLDRSASALAFRGHGPHALPLDKKAAARWMEPAAGAERVPDRFTFTMPA
                     EAAVKVVLTAGMEGTLRSGAASLRVPARRPFRGTLEAGVWTLEAVSERKDSRVEYEVA
                     IVPDPLVAGLARPVTAPAEFTLAVGEAGLYEVASTGGEDVRARLRDESGALVAQNDDR
                     TDDWNFALTTPLSPGRYKLRVDPAGAPRASCRVALRRLEKSVGPEEPLPIARELSPGR
                     GVLEIPVNVPEGTLLEVAAEAPESVGIGISGEDGTLLGQAVGRAPRVAVPAFKAGLAR
                     LSLFSADGRGGKVRLTAKAIPMAAWSERQLAAGGSTAGGAVAVALEAPGVLRFDGPVR
                     VSAAPAAPALPVEGLVAAGGEKLVVAAERPVRALRVRLDENDVHLEARSARVDLAPAK
                     GPVLVRARAALGQPGLALARGAATAGERSGLAAALEAPKAVSLEGDDALSDVVLARLA
                     FAAPRVEKLATLSTEGAIPSRGARAFSLPPGAKRLRVALGAGLFALVTRSGEGLALLG
                     GLEAPSDETLDTDGDRITFLEANGVEARFALDVFEKAVPMAPLAPGAPLDASLPDAGV
                     LRVPLAPPAAGESRMLHVRGASGPATLLASDGRSARGADLAVPTGGGTLLLPHAPGLV
                     LAWVDRPGREAEDLIGAAFDVKSVSLHPPAAPMLSGAVAAFDVDAAGPALVALRAAGP
                     ALTLVAREGTKPEVAIHKAGVSLDAYAPDGKARVVVRALGGGALSGLAAFTATPVTKI
                     GEGLGPETLLGAGQTRLYGFHAARAGAIGLGVRADSDVVSATLLAPDGSRLGEGLVQM
                     PDLAAGDYLLAVRAPGDAAPVRIRPALAGIEPPGTGPPPEVIRTYVMKGPEVQGMSAT
                     RLEESDAETSSPEEETEADGEEESSEDEDEDGEGGPQ"
     gene            complement(17323..17529)
                     /locus_tag="PLP50_09215"
     CDS             complement(17323..17529)
                     /locus_tag="PLP50_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51770.1"
                     /translation="MDLLFDLPPPAAALARRATTVKIRAKAWRVASEADLLRLKKLAQ
                     ASRSSLGDAEDIVFLEARGARSGK"
     gene            complement(17570..17800)
                     /locus_tag="PLP50_09220"
     CDS             complement(17570..17800)
                     /locus_tag="PLP50_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51771.1"
                     /translation="MARAMGRRRGRARARRVSPERRGQIASLGGEARQRSLLATRRLV
                     ENLLYAAAVRELRGEAREVVRMKTFHGRLPGL"
     gene            17955..18248
                     /locus_tag="PLP50_09225"
     CDS             17955..18248
                     /locus_tag="PLP50_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPA51772.1"
                     /translation="MGEPARVGKRGAVVIPAPLRKRFGMGDGTLVVFDEGPDGVLIRP
                     AIAVPVETYSPERKAALILDTAVDAADYRRARKEVRERLGIDPDAVPHSRPPK"
     gene            18245..18664
                     /locus_tag="PLP50_09230"
     CDS             18245..18664
                     /locus_tag="PLP50_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPA51773.1"
                     /translation="MKRTRPFLDANVLFSAAWSEASRLKLLWEVEGADLLASEYVIEE
                     ARRNLEAVEARLRLQGLVQRLRVVPEQLEGLLPAGLELREKERPVLAAALGARATHLV
                     TGDRRDFGRFLGQTVAGVLVLTPAEFLALRRAPSRHR"
     gene            18722..21052
                     /gene="secA2"
                     /locus_tag="PLP50_09235"
     CDS             18722..21052
                     /gene="secA2"
                     /locus_tag="PLP50_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HPA51774.1"
                     /translation="MTAAGFRRLLARLRGETSAEPADDFAPVVRAIGALEEDTAWLTD
                     AELRTRAVVLRERVKQGEPTEDLLVPWFALAREAARRVLGMRPFDVQVLGAAALHAGR
                     IVEMETGEGKTLAAVLPVSLEALAGRGAHVLTANDYLARRDAAWMGPLYRALGLTVAA
                     ITQGSSRDERRAAYAADVTYLGANEAGFDLLRDRLARPPDDVAQRPYHFALFDEADSI
                     LIDEARIPLVIAGGTGIPDGSAVRLAAEVRELKDGLHFERDPEARNVRLTDAGVVELE
                     RRLGSGSLYDAENTPLLAAANVALHARALLARDVDYVVKDRAVRLVDELKGRIADRRR
                     WPDGIHTALEAKEGLPLTREGRVLGSITLQAFAALYPKLSGMTGTAATQAEELRSIYG
                     LSVVVVPPNRPRIRLDRSDLVFARRGDKEQAVVEEIRRLHASGRPVLVGTASVEESER
                     LAALVRGAGAPCRVLNAKNDEEEAAVIAEAGDVGAVTISTNMAGRGVDIRLGGAREER
                     ADAVRALGGLFVLGTNRHEARRIDDQLRGRAGRQGDPGESRFFVSLEDDLFVKHGAAD
                     SLPRRLREWAESGPVRDPAAGKAIAHAQRVIEGQDLEIRRTLLRYDRLLESQRQIFAA
                     RRDEAPTGFLELAAPDRWVAVRDDFGEELASRVERDLTIAWIDSLWSDHLENVAELRE
                     GISLRTLGTQDPLAEYQRALVALFADLLRELDARVAATFETAEITADGVDLGREGLAS
                     PSSTWTYLVNDQPFGSTMERMAKNLVRFAKGMLRPG"
     gene            complement(21018..24524)
                     /locus_tag="PLP50_09240"
     CDS             complement(21018..24524)
                     /locus_tag="PLP50_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51775.1"
                     /translation="MDERIETEAAGELGVEQRIDLAGRIAAMMLLSGRMVFAQAGSSR
                     LDPAADLSVADLARGAMSLGGGPIVQISETFIRELAGSGLFTSRGQGLLGWAQQAHAQ
                     FLCARYLLSSGIPTTQMLRLLRSTDPDHARRVVPQLRGVAAWLASQKREFFEAFVESD
                     PEVLLTLGGDSVPPERKDALALSYLTHVEKRLVNPVRPTIRPRFDGLVHPGIGAALGA
                     VIRDRTRAPLTRRTAVEMALAAKAEELVDVVLPIATDPSEDRYLRHEAIELVAGLGSP
                     EERAVLVPLLNLGREDDPDDEFRGLALWTVWPSLISARELFAALVPPNRPNFYGRYKS
                     FLRNQPIRTLAVNDVPIALEWAARVLRSEEASPLLQLVGPIATIAWRASDDAAVSQSL
                     VDFLTMQFQGHLLISGQRIGKEVAEAVEEDPERRQKIAFHLLRTLEPRHWWKLVASEP
                     PLLRPSDLRELLALARDSKGTDRRTAIVGLISRVLDPSLSDHVDALLETGESIPELSE
                     TILWTFGPVPLKSDTATAMRAQWESSENQRTTAPVLPRSTPQDIVSRLMNERERLGAE
                     ATFPAILDALAGAEGGGSSHGRLTFDLRALGTWRALPADLRREAECAAEAYLSAATTH
                     QEVWLGRDSAPMSAWVALAALRLVATTDLATDQLWKKWAAVTVGLPYYPTEDSEALSE
                     LRRLALAKAREETRAAIRALLSDLGKVRAGYDFESRVGPIWDEWVTAEVVAVLKRPET
                     GPATFQALLEALAAQMPVEAVALSRAMLTDSEPTGPLPAKEASCATILLSTEPAESWG
                     LVWPRLVGAPASLEDVLLSAKRATFRGGKPFYQRLEARQVGEIYRWLVANTPYSGDVE
                     HLGVFTPSRRDELQDLRDEVLRFLSSGGEVATLREFEAIRVEQPELAFLAYYAVDARE
                     RYLERSWIPAAPTELGELFASPARCLVRTGQELSDLILELCVDIQNALGGETPDVDAL
                     WNENAGGDLRPKSERALTNYLANRLRERLGRWGSVILHREVEIKGGLSGAEKTDIYVD
                     AVGDAAVSGSKTVSLVIEAKGCWHQELLSAMETQLVNGYMKTARQAHGLYAVFWFDPT
                     CWEAQDRTRRMKTGAMTFAELREVLASQASDLARSGAQVRSFVFDGSVRRAGPALSER
                     KALRRTGPRRGPTPAAASPSRT"
BASE COUNT         3435 a         8840 c         8782 g         3600 t
ORIGIN      
        1 aagtacctct gcgggggccc cggcgccccg gcgttcctct gggtcccgaa gcgccaccag
       61 gacgtttccc ttcagccgct ctcgggctgg ctcggccacg ccgagccctt cgctttcgag
      121 agcgcctacc ggccggccga ggggatcgcc cgcttcctct gcggcacgcc tcccgtcctt
      181 tcgatgtcgg ctctcgacgc cgccctcgac gtcctcctcg aggccgacct cgcgctcgtc
      241 cgcgccaagt cggagtcgct cacgtcgttc ttcctcgagg cggtggagca ggcgtgcggc
      301 ggcgcgttgc gcctcgtctc gccgcgcgac gcggcgcggc gcggcagcca ggtctcgttc
      361 gcgcatcccg acgcctacgc cgtcatgcgg gcgctcgtcg cccgcggtgt cgtcggcgat
      421 ttccgcgctc cagacgtcct ccgcttcggt ttcgcgccgc tctacgtccg cttcgtcgac
      481 gcctgggacg ccgcgagggc gctcgcggac gtgctttcct cgcacgccca cgagcgcccg
      541 gagttccgcc gccgcgaggc ggtgacgtga gctaccggta gcgggcccgc gtccagtagc
      601 ccgggaccca gcggccccag cggtcgtagt agccgttcac ccagaccgcg tgcaggaagg
      661 gcttcctcac ccagcgcccc tcgacgaacg cccaccggtc gcgatcgcgg tagcgatggt
      721 cgttccgcca cccgtcgtcg cggtagcggt cgcgatggcg acggtccgcg tcgcgccagc
      781 ggtcccggcg gtcccggcgg tctcgccggt cgacccggat gtcgacgtgc ggcggcggcg
      841 ggagaggcgc gtgccggagc acctcgtggg gcggaggcgg cagcggcagg ttcacgtgga
      901 tccgcacgtc cgcgagggcc gacggcgcca ccgcaagggc cagcgccgcc gtcgcggcga
      961 ggaggatgtt cctgtcggcg ttcttcatgt cggacctcct ggggcgttct cgggagctcg
     1021 ttgctcccgc ccttctcact gcaggcccga tgccaggcgc cgcgcccccg aaggtgtgcg
     1081 ccggatcacc ttgagaggcg aaagggcccc ccgcgatggc gcgaggggcc ctcttccggc
     1141 ctccggggag gacggtgcgt tggcgttcgg cgcgttttcg cctacgccgc gtggatccgg
     1201 tcttcgaacg ccgcgagcgc ggccttagct ccctcgccca tcgcgatgac gatctgcttc
     1261 cacggcaccg tcgtgacgtc gccggccgcg tagatcccct tcgtcgaggt gcggcacttc
     1321 tcgtcgacga cgatctcgcc gaagcggttc acgtcgacga ggccgcggac gaggcccgag
     1381 ttcggggcga ggccgatctg gatgaagatg ccgtcgatcg ggaaggtcct ttccgcgtcg
     1441 gtcgcgcggt cgacgacctt gatcgccgtc accttgctcc cgtcgccgag gatctccgcc
     1501 gtccggtggt tcacgatcac ctcggtgttc gggagcttct tcaggttgtc gacgaggacc
     1561 ttgtcggcct tcagctcggc catgaactcg acgaccgtca cgcgcgagca gatcccggcg
     1621 aggtcgatcg ccgcctcgat gcccgagttg ccgccgccga tgacgacgac ctcccggtcc
     1681 ttgtagaacg gcccgtcgca gtgcgggcag aacgcgacgc cgcggccgat gtactccttc
     1741 tcgcccggga cgttcagctc gcgccactgc gcgcccgtcg ccatgacgag cgccttcgtc
     1801 cggaggacct cgcctccggc gaggcggacc gtcttcaggt cgccgtcctc gatcgcctcc
     1861 acgcggcggt tgtcgaggac ctcgaccggg tagctccgaa ggtgcgagag gaggtcggcc
     1921 gcgagcctct tcccctcggt gcgcgtcgtc ccgatgaggt tctcgatccc gagcgtctcg
     1981 aggacctgcc cgccgacgtt cttcgcgacg agcgccgtcc ggagcccctt gcgcaccgag
     2041 tagatcgccg cggacgcgcc cgccggcccg cccccgacga cgaggacgtc gtacgggtct
     2101 cgctcgggga aggcgcccgg cgcctcggag ggcgccgagc cgaaacgctc ctcgagcgcg
     2161 tcgaggagct ccgcgaggcc ggcccggccg acggagacct gcgcgttctc gtgaacgagg
     2221 gagatgacgg tcgggacgcc ctggatgccg agcttctcga cctcgccctc cgcgaggccg
     2281 ccgtcgacca tttcgtggac gaagtcggga tggatcaccg ccatcaggtt cagcgcctgg
     2341 accacctcgg ggcagttgtg acacgacaga gagacgtacg tccggaggcg gaccggcccc
     2401 ttcaggtgcc ggatccggtt ctggattccc tcgtcgggcc gcttcccctg cccggcggcg
     2461 tacttcaccg cgaggacgag cgacgagaac tcgtgaccgc ccggaatccc gcggaagagg
     2521 atcgtcgccg cgcgcccctc ggcggccacc tcgaacgcgg ggatcgcgct cggctccccc
     2581 tcgacgaggt ggacgatctt cccgggcgcc gtccccgcga cgtccgcgag catcgtcgtc
     2641 agctccccgt tcttcgggtg ctcggacgga cgctgccgga ggacgaccgt cacggggagg
     2701 tcgctgtaga tctgctggag ctgcgcggcg agagcggggt cgagcatcgt catcctcttc
     2761 gggggtgtgc gggagtcgag gcgggaagga aggccgttcg gcgcgcggtc acgcgcctcg
     2821 agcggccgga gggggacccg gcgttcccgc cgggtccccc gtggaccggg acgtgaatca
     2881 gatcttgccg acgagatcga gcgagggctt gagcgtctcc tggccggggg tccacttggc
     2941 cgggcagacc tcgttcgggt ggctcgcgac gtactgcgcc gcctgcacct tccggaggag
     3001 ctccttggcg tcgcgcccga tgccgttgtc gtggatctcg gcgatcttga cctgcccctc
     3061 ggggttcacg acgaacgtgc cgcggtaggc gagacccgcc tcgtcgatgt ggacgccgaa
     3121 gcccttcgag aggatgtggg tcgggtcggc gagcatgacg tactggatct tcttgatcgt
     3181 gtcggaggcg tcgtgccacg ccttgtgggt gaagtgggtg tcggtcgaga cggagtagat
     3241 ctcgacgccc atcttcttga actcggcgta gttgtcggcg aggtcgccga gctcggtcgg
     3301 gcagacgaac gtgaagtcgg ccggatagaa gaagaagacc gaccacttgc cggcgaggtc
     3361 cttgttcgag acctccttga aggcgccgtt gtggtaggcc tgaaccttga actcggggat
     3421 ctggctgttg atgacgggca tgctcgttct ccttgcgttc tgatgcgggc gggtcggccc
     3481 actttttaga ccttttctaa tttatgacaa gagggaggaa aaggcaagcc cacgttcggt
     3541 cgattcgttt tcccgggctc ggggcgcgcc tcgagcgggc gcactcgccc acgccgccgg
     3601 ctccggccgc cacgtcgctt gacttcccat cgtgcccccg gtatgttccc cctcccctga
     3661 cgcggggtgg agcagcctgg tagctcgttg ggctcataac ccaaaggtcg caagttcaaa
     3721 tcttgccccc gcaaccaatc agaagtcgaa gggcgccagt cagttacgac cggcgccctt
     3781 cgtgttttcg ggtgccctga agacagaggt cggcgggctc actccacccg gaccgtgacg
     3841 cccggcgagg cgagcctcac gaacgggagg cgcgccacga acgtgtgctc gccgggggtc
     3901 accttccagc gaacggtgta gggatagggc gcggtcgcgt acggttcgcc atccacccac
     3961 cagacgatct cgcgcgccgc gggtgagacg acggccttca gggccagggt cgcgttgtcg
     4021 gcgggagtct ccgggtcgcg caggacgcgc aggccgttcg cgggtgaggt gatcctcacg
     4081 cgcgccgtcg tccccgcgac gagaggcggg gcgcccgatg tgaaggcgcc cccagaggtt
     4141 tcgcgccggg cgacgagtcc gggagggcgg ggcagcccgg ccgccgcgct ccaggccgcg
     4201 tactgcgggc cgaggtcgac gaaggtccgg acctccacga agcgacgcgg cgtcgcgcgc
     4261 gtggcgagga ggcccgagcg ggcgtcgacg gcgagcttca cgtgcgcggc gcattcgtcg
     4321 agcggctcct gcccggggcg gatccactcg gtcaccgtcc tctcgcaggc gtccgtggca
     4381 aggcggccgg tcagggcgca gagccgcacg ctcaccgagc cgcgcggcgc ggggaaggag
     4441 aggtcgccga ggccgtccgt ctcgccgtcg tggagcgaga gcatcacctt ctgcgcgagc
     4501 tccgcggcgg cgcgatagcc ggagagccgg ttcatcggct tgaagtcggg gtgccccacc
     4561 cagacggcga cgaggtagcg cgtcgagaag gccgcggtcc aggcgtcgcg gtagcgcgac
     4621 gacgtcccgg tcttcacggc gacggggaac gggtattccg tcacccccat ccgcgggaag
     4681 ctcgggaggc gcgccatcgg gtcggcgagg tagagcgtca cggcccgcgc gctctcctcc
     4741 gagaggagcc gccgcgggcg agggagcggc tgcccctccc agaagagcgg ctccacgagc
     4801 gtgccgtcgt tcgtgaggac gctgtaggcg cggaccatcc cctcgagcgt caccggcagg
     4861 ccgccgatcg cgaggccgag cccgtagcga tggaccggga tcgacccgtc gtggagaccg
     4921 aggtccctga ggaacgcgta cccctccgcg aggccgaccc ggtcgaggag gtcggccgcg
     4981 gggacgttgc gcgagttcgc gagggcgacg cgtggcagga gcgggccgag gaagatgtcg
     5041 tccgcgttcg tgatcccgcc ggccccgcgg tcgaggtcgg ggaggatcgt cgccggggtg
     5101 atcgtcccgc gctcgagcgc gagggcgtag aggaacggct tcaacgcgct cccgggcgag
     5161 cgggggacgc gcgcgtagtc gatggcgccg gcgtgctcgg cgtccgccca tcgcgtcgag
     5221 ccgacgcgcg cgacgatcgc gttccgtttc cggtcgacga cgatcaccgc cgcgttcccg
     5281 gccccctgct tctcccagat cgtcacggag tcgcgcgcgg cgagggcgac gcgccgctgg
     5341 agcggaaggt cgaccgtcag cttcacgacg gtccgtccgg cgagcgcctc gcgccctccc
     5401 ggcgaccgcg cctcctccgc gaagcggagg agggcgtgga tcgcctccgg cggccgcccg
     5461 cggcgcggcg gcacgcggag gctcgcgatc tcgcgcgcgg cgagcgccag ctcggcgtcg
     5521 gtcagctcgc cgcggcggcg gaggagccgc aggatccgct tcccgcgcgc gaccgcccgg
     5581 agccgcccgg ccgggtcgaa cgggttcatc cggcgcggcg actgcgggat cgccgtcagg
     5641 aacgccgtct cggcccacga gaggtcctcg acgggcttgt cgaggtagcg ccgcgccgca
     5701 taggcgatgc cgtggacgcg gttcccgtac gggacgatct cgaggtagtg gcgcagcacg
     5761 ccctggcggc cgtagcgcac cgtgaggagg aacgccgtca ccgcctcctg cgccttccgg
     5821 atcagcccgc gccggccggg gtgctggagg cgtgcgacct gcatcgcgag cgtcgaccct
     5881 cccgacaccc gcctcgcgtg gcggacgttc tgcgtcaggg cgcgggcgat cgccgcgagg
     5941 tcgacgccgg gatgccacgc gaagcggcgg tcctcgaccg cgatcgtcgc ggcggccacg
     6001 cggggcggga cggcggggag cctccagtag ccgagctgcg actgtccttc gtcgagggtc
     6061 acctcgccga ggaagaggtc gttccggtcg cgcaggagga gcgtcggctc gggtgagacg
     6121 aagcccgcgc gccagacccc gggcccggcg acgagaagcc ccagccccgc cgtgccccag
     6181 aggagggcac ggcggagagg gaggcgccgg aggagcgcgc gggcgtccac ctactgcggg
     6241 cgcgagacct cgatccgcgc gccgttcgag ctcccgcgca cggcgccgtc gtacatcagc
     6301 tcgcacttcg cggggggctg gacgaatgag ccgggcgtcg tcgcgcgggt ccggaagcgg
     6361 aagtcgtacg tcccggccgg cagcgaatcg tagaaccagg agacgctgtc gtcgaggaac
     6421 ttcacgtagg tcggcgcgag cgtcgggggc ttgctcgggg tcgcctccgg cggcgccgtc
     6481 gcgagcgccg ggttgagcgg ctccatcccc gcggcgagcg ggacggtgat cgccacgtgg
     6541 ttcctcttct ccgggttcac gacctgcacg tggtcctcga cgaccgtccc caccgcgagc
     6601 gggatcgtct tccccggctc ggcgagcgcc gtcttctcca tcgggccgtc ggccttcacg
     6661 accgcgacct cccgggagac gacgaacccg ccggaggcgg ccggagcccg gctgccgtcc
     6721 tcggcggcga cgtagcgcgt ctcggccatc acgacgaggg gccgggaggc cccgctcacc
     6781 gcgatcgcgg cttcggccgg cgcggtggag acggtcttcg cgacggcgct gccgctcgcg
     6841 gaggcgagcg ggcgcgagct cgcaccctcc ttcaccgtgc acgtgaaggg ctcgaagcgc
     6901 ccgctcttca ggacgtccga gagggcgagg agcgcctccg cgttcgcgtt cgtcgttccc
     6961 cagccgtcgc cgcgtccgcg gacgacgagg ccgttcacga gcgccgcgat gcgcgggtcc
     7021 tccggcgcca cgcgctggag cgcgcggagc acctcggcca gcgtccggtc ctcgctgcag
     7081 aggaggagga ggtcgccgag ccggtccccg tcctggaggc cgccgtagac cggcttccct
     7141 tcgcggctcc ggaagacgac gccttcccag agggcgttcg ccatgccgcg cgagacctcg
     7201 ctcttcgcat ccggcgagcg cgagaaggcg aggagcacct gggccttccc ctcgaggcga
     7261 aggacctggg gctttcgcgc cagctcggcg gcgtaggccg cgtcgaacct cccggcgtgc
     7321 gcgagcgcgg agagcgccca cgtcctctcg aggtacgact ccccgccgac gaagtgggcg
     7381 taatcggagc ggagcgcctt ctcgagcgcg tcaaggagcc ggtcgaggag ccgggcgtcc
     7441 gtcgcgaagg aggcctcctt cgcctcgacg aggaagtcga cgctccaggc ggtcagcgcg
     7501 acgtagcccg tgctcccggg ccagtacgcg acgagcccgt tcgcatcgac cgcgtcggcg
     7561 atcgcctgct gcgcggtcgc gaaggcgcgg ctcgcgcgat cctcgtctcc cgtgcggccc
     7621 aggaccccgc ggagcttccg cgccgccgcg agcgcccgcg ccgcgctcac cttctgctcg
     7681 gtgcagccgt acgggtatgc gtcgaagaag tcgagcccgc tcgccatctt cacgacggcc
     7741 ggctcggagg agaggaggag cgagcgcttc agcgtcccgg cccggacggg ctcctggatc
     7801 tccgggacct tgaactccgc tcccggcgca aggtccgcga cgacgcgccg cgcgaccggg
     7861 tcacggtcgg ggaggatcgg gagcttcacc tcgaacgcgt ccgacgcctt gtcgctcgcg
     7921 cgctcgacgc ccgcctggaa cgtgatcgga cccgtgccgt tcgccggcac ctttacgccg
     7981 aactcgattc gctcgggctt gttcggctcc atcgtgaggg agcgcgtggc agggccgtcg
     8041 agcatcgcgc ccgtgacctt cacctgcgcg gaggcggggc ctcccttccc ttccacgatg
     8101 cgggccacgg cgacgcccgt gaagacgtcc ccggcacgga ggaagcgggg gaacgccggc
     8161 tggacgagga cggggaggcg caccgcgatc gtcccggtcc cgtatccaaa gcggtcgggg
     8221 ccgctcatcg ccttcgcgcg caccttgaag ttcgtcaggt tgtcggggag cggaacctgc
     8281 accgtcctcg tgccttcggg cccgacgacg atcgccgggt cgtagaacgg cacggtcttg
     8341 aagttcttcc tcaccgtggc gcggtcgagg actccccctt cctccccctc gtcgccgccg
     8401 ggcgcctcgg cgaacggcag caggccgaag accgagttgc gcgtgtcgcg cgcgacgagg
     8461 tgcgacctca ccggcgtgat gaagtcgggg agcgggtcga gccgctgctc cttgccgagc
     8521 gccaggaccg cctggtcgac gagccagagc gtcacctcgc ctgaaagcgg cttcccggcc
     8581 gggtccttca gcgtgatcgt cacgtcgacc gtccgccccg gcatcgagac ctccgggtgc
     8641 gcgagcttca cttccacgcg gttcgccgtc ggctccacct ggagccaggc ggtcgcggcc
     8701 atcgtctccg gcttgccgag gtccgtcgcg ttcccctgcg cggggcccgt ccccgggatc
     8761 cgcccgcgca tcaggacgaa gtggaccggc acgcgcggca cgaaggtcgg gaggatgtcg
     8821 agcgggaacg tcgccgctcc cttctcgacg tcgatccacg agtagcggtt cccctccggc
     8881 gcctcgacga cggcgagcgc gcgcgccctc tggaacgggc tctggaggac gatcgcggcc
     8941 ttcgcgcccg gctggtactt caccttgtcg ggggcgacgc tgaagacctt cgtcgcgggg
     9001 cgcgcccagg tcatcggctc gtcgccgccg gcgtagaggt cgacggagat cacctgcgcc
     9061 cggccgagcc ggtcgcgcgc ctccagctcc acgacgtaga cgcccgaagt ctcgatcggg
     9121 agcgggacgg tgaggggctc cttgccgctc ttcaccgtct gctcgctcac cttctcgtcg
     9181 acgacatccg tcaggtagcg ggcgacgccg tccgagaagt cgctcgcgcg gaggaccgag
     9241 tgccactgcc gcttcaggag ccggacggtg atcgccttcc cctcgagcgt cttcccgtcg
     9301 gggccgacgg cgatcacgcg cggcgagagc gccttcgcgc gctcgacgaa gcggggaacg
     9361 tcgaggccca gtacgaaggg cgggagcgcg gcgacccggc gcgtcgacgt caccgtctgg
     9421 tcgtcggcgc cggtgaccgt cgcctcgacg acgtaggtgc gcggctgcgc ggtcggctcg
     9481 atcgtcgggt cgagcttcag cgtcgccccg cccgcgtcgt tcgtcacgcc ctccttcgtc
     9541 gtctgcggcg acgagcggaa gcgctcggtg cgggagaagc ggccgtcgga cgagaaccgg
     9601 aacccctcgc gcgcgggcgg ctgccagtcg gtggggaact gcgtgacgcg ccactgcacc
     9661 gggcgcccgc tcaccctccc gccggcgtag tacgtcgccg tgagcgagac ctcgaacgcc
     9721 ttgtcgagcg ccgtccggtc ggggccgtgg aggtccacct cgaactccgg caggcggtac
     9781 gcctccatcc ggaagctcac gtggccgaac ttccgctgtc ccttgtcttc gagatgcgcg
     9841 tcgtagacgc cggtcgggag gttcttctcg ttgaagagcc tgtagaagct ccccccttcg
     9901 gagagcgcga cggggaagcg ccaggtcatg tcgcccggtc cgctcacgac gagcgtccac
     9961 tcccccgccg ggagcggatg gagccgcccc gccacgcgct cgcggaggta gcccttgagg
    10021 tggacctcct cctcggggcg gtagacgggg cgctcggtga agaggtggca gagcgtctcg
    10081 gccatcgggc cgcgcccttc caggacctcg aacgcccagt cgagccacgt ctcgcggctc
    10141 cggctccact ggttgtcggc gtacttctcc ggcgggtgcg cggcgtcgac gacgaggacg
    10201 tccttcccct tcgtcaccac gatccgccgc atcgtgcggc tctcgcgcag gtcggggcgc
    10261 cccggcgcct tccagtggaa cgtcccgtcg gggccggtcg tcccctcggc gaacgtctcc
    10321 cacttcgcgt cgttcgggcg gcggtgcgtc ccctcgaggc gcaccgtcgc gccggcgacc
    10381 gggagcgccg tcgagagcga ggtcaccgcg aatcggaccg cgtacggctc ctcgagcgtc
    10441 gagagagaga ggtccgtcgc ctgaacgcgc atccagctcc tcaggcttcc cccttcgagc
    10501 gggcggagac cgacgaggta ggtgccgggc tcgcccggcc ccgagatctt cgcgaggtgc
    10561 ggcgtgaggt cgaagccgaa ccgcgccgcg ccgccgtcct tccggagcgg aagcgtgacg
    10621 atcgtcgaga cgggcggcga gccgagcagc cggaggtgcg gctcgatccc gtcgggtggg
    10681 atgttctggt cgggggcgtc ccacgccttc ggctcctcgc cggggcccgg cgggcgctcc
    10741 gactcgtcga cggaaacgcc ccggtccggg aagggccaga ggctcctctc gagcgggtcg
    10801 atcttctgga ccctcacgtc cagcttctcc gcgccgcgcc cttccaccgg gaccatctgc
    10861 gcgccgaggc gctccacgac gccctgcccg gcgccccact tcacgaacga ggcgcgctgc
    10921 gggaagacga cgaagagctc gctccttccg gccggctcga gcgcccgccc gcgggcgtcg
    10981 ctcatcttcg tcgagaggag ggtcacggcg tagagcgtgt cgcgccggaa cttcccggtg
    11041 gcgacgagcg tgcgggccga ggtctcgaac ttcaggtcgt ccaccgcggg ggtgaagcgg
    11101 acgaggttcc tcgcctcgac cggcgagatc gggcgcgggc tcgacgagaa ctcgacgagg
    11161 accgccggcg cctcggagcc gcacctcagc gcctggtcct tctcgtagag cgtgcccgcg
    11221 ggggtgaccg ggagctgccg ctcgcggcag ccgaaggaga cgacccggaa cggctcgccc
    11281 gtcgagaacg agagctcccg ggcggctccg gccgccgcct cgtcggccga gagcttcagc
    11341 gtgaggacgg cgcgcgtgcc ggccgggacc gcgtgcgcga gcgacagggc gtaggaggcg
    11401 gcgtcggtcc ggctcatccg ctcgagcggc ttgatccgga tctcgtccgg cggaacgcgc
    11461 cggccgcccg acgttcccgg aagcgggcgc agctcgaggg cgatcatctt cgcgagcgcg
    11521 gccggaggaa gcggctcggc gaaggtcagc gtgatctcgt cgaacggctc gagcccttcg
    11581 gcgccgtcgg gaggcacggt cgacgccggc gcatcgagga gcgtcgcgag cgtcgtcgcc
    11641 ttcgacccgg cgggcgtcac gcggaagcgc gagagcgccg gccacggctc ggcgggacgg
    11701 aactgcagcg tcttcgcgtc gagccagcgg aaggcgcccg gctgcgccgg gtcgatcgtg
    11761 gcgaaacgcc cggcgtcgtc ctcggggccg ccggccttcg ggcctgtgtc ggcagagaag
    11821 aagacggtca ccgggtccca gcggcggagc aaccggtcgg ggacgacccg cgcggcgggc
    11881 tccgcggaat tcgcgcggga accggccgcc gaggccttga cgcccacggt ggagagcgcc
    11941 agggagagaa gagcgatcgg aaccgtcccg cgcttcactg cggacctcct tctccgtcct
    12001 cgtcctcgtc ttcggaggac tcttcctcgc cgtccgcttc cgtctcttcc tcgggcgagg
    12061 aggtctccgc gtcgctctcc tcgagccgcg tcgcgctcat cccctgcacc tcgggcccct
    12121 tcatcacgta cgtccggatc acctcggggg gcgggccggt ccccggcggc tcgatgccgg
    12181 cgagcgccgg gcggatcctc accggcgcgg cgtcgcccgg cgcgcggacc gcgagaaggt
    12241 agtcgcccgc cgcgaggtcg ggcatctgga cgaggccctc gccgagccgc gagccgtccg
    12301 gcgcgaggag ggtcgcagaa acgacgtccg agtcggcgcg cacgccgagg ccgatcgccc
    12361 ccgcgcgcgc ggcgtggaag ccgtagaggc gcgtctgccc cgcgccgagg agcgtctccg
    12421 gaccgagccc ctcgccgatc ttcgtcacgg gtgtcgccgt gaaggcggcc agccccgaga
    12481 gggcgccgcc accgagcgcg cggacgacga cgcgcgcctt gccatccggc gcgtaggcgt
    12541 cgaggctcac gcccgccttg tggatcgcca cctcgggctt cgttccctcg cgcgccacga
    12601 gcgtcagcgc gggtcccgcg gcccggaggg cgacgagcgc cggcccggcg gcgtcgacat
    12661 cgaacgccgc gacggcaccc gagagcatcg gcgccgcggg gggatggagc gagacgctct
    12721 tgacgtcgaa cgccgcgccg atgaggtcct cggcctcccg cccggggcga tccacccacg
    12781 cgaggacgag ccccggcgcg tgcgggagga ggagcgtgcc tccgcccgtc gggaccgcga
    12841 gatcggcgcc gcgagcgctc cggccgtcgg aggcgaggag cgtcgccgga cccgacgcgc
    12901 cgcggacgtg gagcatccgg ctctcacccg cggccggcgg cgcgagggga acgcgcagga
    12961 ccccggcgtc ggggagcgaa gcgtcgagcg gcgcgcccgg agcgagcggc gccatcggca
    13021 ccgccttctc gaagacgtcg agggcaaacc gcgcctcgac gccgttcgcc tccaggaacg
    13081 tgatccggtc gccgtcggtg tcgagcgtct cgtcggaggg cgcctcgagc ccgcccagga
    13141 gcgcgagccc ctcccccgag cgggtgacga gcgcgaagag ccccgcgccg agcgccacac
    13201 ggaggcgctt cgcccccggc gggagcgaga aggcgcgcgc gccgcgcgac gggatagccc
    13261 cctccgtcga gagcgtcgcg agcttctcga cgcggggcgc ggcgaaggcc aggcgcgcga
    13321 ggacgacgtc ggagagcgcg tcgtcccctt cgaggctcac ggccttcggc gcctcgagcg
    13381 cagccgcgag gccgctccgc tcgcccgccg tggcggcgcc ccgcgcgagc gcaagaccgg
    13441 gttgccccag cgcggcccgg gcacggacga ggaccggccc cttcgccggg gcgaggtcga
    13501 cgcgggcgct ccgcgcctcg agatggacgt cgttctcgtc gaggcggacg cgcagggcgc
    13561 gcaccggcct ctcggcggcg acgacgagct tctcgccccc cgccgccacg agcccttcga
    13621 ccggaagcgc cggggccgcc ggtgcggcgc tcacccggac cgggccgtcg aagcgcaaga
    13681 ctccgggcgc ctcgagcgcg acggcgaccg cgccgcctgc ggtcgagccg ccggccgcga
    13741 gctgccgctc gctccaggcc gccatcggaa tcgccttcgc agtgaggcgg accttcccgc
    13801 cgcgtccgtc ggccgagaag agggagaggc gagcgaggcc cgccttgaag gcgggcaccg
    13861 cgacacgcgg cgcgcgcccg acggcctgcc cgaggagcgt cccgtcctct cccgagatgc
    13921 cgatcccgac gctctccggc gcctcggccg cgacctcgag gagcgtcccc tccgggacgt
    13981 tcaccgggat ctccaggacg ccgcgaccgg gcgaaagctc gcgcgcgatc ggcagcggct
    14041 cctcggggcc cacgctcttc tcgaggcggc ggagcgcgac ccggcacgag gcgcgcggcg
    14101 cccccgcggg gtcgacgcgg agcttgtacc ggccggggga gagcggcgtc gtgagggcga
    14161 agttccagtc gtccgtccgg tcgtcgttct gggcgacgag agcgcccgat tcgtcgcgta
    14221 ggcgtgcgcg gacgtcctcg ccgccggtgg aggcgacctc gtagagcccc gcctcgccga
    14281 cggccagcgt gaactccgcc ggcgctgtca ccgggcgcgc gagccccgcg acgagcgggt
    14341 cgggcacgat cgcgacttcg tactcgacgc ggctgtcctt ccgctccgag accgcctcga
    14401 gcgtccacac acccgcctcg agcgtcccgc ggaagggccg ccgcgccggg acccgcaggc
    14461 tcgccgcgcc cgagcgcagc gtcccttcca tgccggccgt caggacgacc ttcacggccg
    14521 cctccgccgg catcgtgaac gtgaaacggt cgggaacgcg ctcggcgccc gccgccggct
    14581 ccatccaccg tgcggcggcc ttcttgtcga ggggaagcgc gtgcggcccg tgcccgcgga
    14641 acgcgagcgc cgacgccgac cggtcgagcg agacgacgac gcgcgccggc accgccgtcg
    14701 ggaggacgac gagccggtac gtccccttcg gaagcgtctg gcggaggtcg ccgtcgatcc
    14761 ccggcttcag gaggggccac ccttccgcgt cttcgagccg gaccgcgaac gtccgcttga
    14821 agccccgggc gcggatccgg tagctccccg cgcgcttcac gtcgaggacg agcgacgccg
    14881 cctcgcccgc gtcgagcgag aggcgcgccg ggacgccgtc ggtcacggcg ccgccgtcca
    14941 cgacccgggt cttcgtgagg ccgacgccga ggtgccccgc cgagaggccg cgcggctgca
    15001 ccgtcacctg gtagtccccc tcgcgcagga agtcggcgac gagcgcgttc cggcccacgc
    15061 cgttctccgc ctcgctccgg aactgggtga ccgtccgggt ccggaggagg gcgcccgtcg
    15121 cgaggagccc ggtcgtctcg atccggtaga gcgccggctg cgggacggtg acgttgaagg
    15181 tagccgtctc gtccttcgcg aggtcgagcc cctgggcgac tccttcgagg agcgccggga
    15241 acttcggcag cgccgcgagc gcgccgtcgg gaagcggcgg gagagggacg tccttctcga
    15301 aggcgcgggg ccgcgcggcg agcgagacga cccgcggcgc gtctcccccg agcgtcaccc
    15361 tcgcttcgtg gcgtcctttc gccacgcgcg gtcggggtac ggggggcgcc ccgtcgaccg
    15421 agatcgggag cgcgccgccg tcctccgtct cggcgatgag ggtctgttct tcggtcgcct
    15481 cgaacgggac gctgatcgtc tggcccgggc ggaccgcgac cgggacgggc tcggtcaggt
    15541 ccatcgggag cgagcggaga acgaggcccg agacgacgcg cggcctcgtc cccacgacga
    15601 gggtgtagcc gtgccggtcc gagagggcga ccgaggggaa gaggattcct ccccgcggcg
    15661 cggcgggagc gggcgccttc tccttctccg cgtggagcga gagcgtgacg attcccttct
    15721 tcaccggctg cgccgtgagc tgccagtagc ccgggtcgag gtcgacggtg ccgcgcccgc
    15781 gcagctcgaa cggcttcagc tccggcgttc cctccccgag cgcggggagg acccggatct
    15841 tcgcgtcgac ccctgccgcg tcgatcacgt acctgccggc cgagccgacc tggaagaaga
    15901 gcgtcagctc gccgtcgaga ttcccgcgac cggcccatgg cttcccgagg tcgatctcgg
    15961 ccgcggccgt tcggatcggc gtccgcgaga cgtcgtcgag gaggaggccg gtctggtcga
    16021 cggcgtcgcg cgggtcggag gacgggatcg tgccgaccca gtaatcgccg ctcccgcgga
    16081 tgggataccg gtcgcgtagc tcgaagtgcg tgaggacgta gggctgcccc gcctcgccgc
    16141 gtacggtgac gagcgtgagc ccgtccgtcc gccctccggt gcggatcgcg gcttccggcg
    16201 ggttcgtctt cttgtcgatc gctccccgct cgcccccttc gacgaagggc ctcgactccg
    16261 tccaatcgct cgcgctgaag gagatggctc gcgcttccgg cacctcgagg ttgaagtacg
    16321 tcgcaccacc gccgacgaga tagcggtcga tgccggaggg ccccacgacg tggcgcgcgc
    16381 gcccggcgat tccgacccgc ggaagccccc accgtaggtg gagcgggctc gcgtcgctct
    16441 cctcggccca cggctcgccg gggccgccgt aggcggaaag gagatacaga ccgggagcga
    16501 ggtcggccgc gaggcggatc acccggagcg gacgccccgg ctgcggcatc gtgacctcgc
    16561 tctcgggctc ggcggcgtgg agccacgacc cgtcgcgcca gagccgcagg tcggtgaggc
    16621 ggcggccggc cgcctcgaac gccacgtatc gccgagcctc gacggagatc caccatgagc
    16681 gctgctcgac gtcgccgagc gtcgtctcga ccggcttccc ctcgacgagg cgcagcggcg
    16741 tcgcgccgtt cccctcgacg aacggccggg ccgagagcgt cgcccgcccc ttcgccttcg
    16801 ggtcgccgtt caggacgagt ctgacctccc cgcggtcgag gaagacgtcg gcccgcccgt
    16861 ccgtctcccc cggcgtcccg tttcgctcgc cggggcccga catccggtcg acgaccgtca
    16921 gcgccacgcc gctcttgctc gcgaccgcga gcgagtaccg gccgaagcgc ggcaccgtga
    16981 cgaccgccgt ctgccgcccc gccgccggta cggcggcggg ggcaacgcgg acgtcgtcgg
    17041 cggcgaggag ctggggagcg aggaacgaga gaagaagggc ccttctgatc acgcgtcctc
    17101 cgtcgactgc gctgcgcctc acgcctgcgg gagggtcgtc cgccgatccg ggtcgatgcc
    17161 gcggccaagc catggccacg cggaccggga acccgggcga tgggaccgga ggcgtcgtgc
    17221 agctcaagtc ggttcggcgg aatcatcgcc gattcgtcgc gtcgtcgctc gacgtcttcg
    17281 aggcctgatg ctgctgaagc ctcagctcgc ggtcccgaac gctcacttcc cgcttctcgc
    17341 cccgcgagcc tcgaggaaga cgatgtcctc cgcgtcgccc agagaggaac ggctggcctg
    17401 cgcgagcttc ttcagtcgca ggagatccgc ttccgaggcg accctccagg ctttcgctcg
    17461 gatcttgacg gtggtcgctc gccgggcgag cgctgccgcg ggcggcggga ggtcgaacag
    17521 caggtccacg cgcaggcccg ttctcgggga cctgtgagcg ttttcgctct tagaggccgg
    17581 gcagccgtcc gtggaacgtc ttcattctca cgacctcccg cgcctccccg cgaagctccc
    17641 ggaccgccgc ggcgtagagg aggttctcga cgaggcgcct cgtcgcgaga agcgagcgct
    17701 ggcgcgcctc tccgccgagc gacgcgatct ggccacgccg ctcgggggag acacgtctcg
    17761 cccgggcgcg tccgcggcgc cgccccatcg cacgggccat cgctcccaca tccacggtcg
    17821 cactctactt gactccaagc agcaggcagc ctctctgaat gggctggcgg gagccgccga
    17881 tacccctcgg cggggattcg ggtatcctcg cgccaggcgc ccgattctct gatattcggg
    17941 cgcgggaggc agccatgggt gagcccgccc gggtgggcaa gcgcggcgcg gtcgtcatcc
    18001 ccgccccgct tcgaaagcgt ttcggcatgg gcgacggcac gctcgtcgtc ttcgacgagg
    18061 ggccggacgg ggtcctcatt cgtcccgcca tcgccgtccc cgtggagacc tactccccgg
    18121 agcggaaggc ggcgctcatc ctcgacacgg ccgtcgatgc cgcggactac cgcagggctc
    18181 gcaaggaggt ccgcgagcgg ctcggcatcg accctgacgc cgtccctcac tcccgaccgc
    18241 cgaaatgaag cggactcgcc cgttcctgga cgccaacgtc ctcttctcgg cggcctggag
    18301 cgaagcgtca cgcctgaagc tcctttggga ggtcgaagga gcagatctcc tcgcgtcgga
    18361 gtacgtcatc gaggaggccc gacggaacct cgaagccgtc gaggcccgcc tgcgcctcca
    18421 ggggctcgtt caacgcctcc gcgtcgtacc cgagcagctc gaagggctcc tcccggcggg
    18481 gctcgagctc cgcgagaagg aacgacctgt cctcgccgcg gcgctcggcg cgcgcgccac
    18541 ccatctcgtc acgggcgatc gccgcgactt cgggcgcttc cttggccaga ccgtggcggg
    18601 agtcctcgtc ctgactccgg ctgaattcct cgcccttcgg cgcgcgccat cccgccacag
    18661 gtagccgagc tcgaggtcct cctgacggac gcagggaggc gcggctggga caatcgaccc
    18721 gatgacggcg gcaggattcc gacggcttct ggcccgcctc cgcggcgaga cgtccgcgga
    18781 gccggccgac gacttcgccc cggtcgtccg ggcgatcggc gcgctcgagg aagacaccgc
    18841 ctggctgacc gacgcggagc ttcgaacgcg cgccgtcgtc ctgcgcgagc gcgtgaagca
    18901 gggcgagccc acggaggacc tcctcgttcc ctggttcgcg ctggcccggg aggcggcgcg
    18961 gcgcgtcctc gggatgcgcc cgttcgacgt ccaggtcctc ggcgccgccg cgcttcacgc
    19021 cgggcggatc gtggagatgg agaccggcga gggaaagacg ctcgccgccg tcctcccggt
    19081 cagcctcgag gcgctcgccg ggcgcggcgc ccacgtcctg accgcgaacg actatctcgc
    19141 gcgccgcgac gccgcgtgga tggggccgct ctaccgggcg ctcgggctca ccgtggcggc
    19201 gatcacccag ggctcgtcgc gcgacgaacg ccgcgccgcg tacgcggccg acgtcaccta
    19261 cctcggagcg aacgaggccg gcttcgacct cctgcgcgac cggctcgcgc ggccaccgga
    19321 cgacgtcgcc cagcggccgt accacttcgc gctcttcgac gaggccgact ccatcctcat
    19381 cgacgaggcg cgcatcccgc tcgtcatcgc cgggggaacg ggcattcccg acggctccgc
    19441 cgttcgcctc gcggcggagg tgagagagct gaaggacggc ctccacttcg agcgcgaccc
    19501 cgaggcccgg aacgtgaggc tcaccgacgc cggagtcgtg gagctcgaga ggcgcctcgg
    19561 gtcggggagc ctctacgacg ccgagaacac gccgctcctc gccgcggcga acgtcgccct
    19621 ccacgcgcgg gcgcttctcg cccgggacgt cgactacgtc gtgaaagacc gggccgttcg
    19681 cctcgtggac gagctgaagg ggcggatcgc cgatcgccgg cgctggccgg acggcatcca
    19741 tacggccctc gaggcgaagg aagggctccc tctgacgcgg gaggggcgag tcctcggctc
    19801 catcacgctc caggctttcg ccgccctcta tccgaagctc tcggggatga ccggaacggc
    19861 cgcgacgcag gccgaggagc ttcgctcgat ctacggcctt tccgtcgtcg tcgttccccc
    19921 gaaccggccc cgcattcgcc tggaccggtc ggacctcgtc ttcgcgaggc gcggcgacaa
    19981 ggagcaggcc gtcgtcgagg agatccggcg cctccacgcc tcggggcgcc cggtcctcgt
    20041 cggtacggcg agcgtcgagg agtcggagag actcgcggcg ctcgtccgcg gcgccggcgc
    20101 cccgtgccgg gtcctcaacg cgaagaacga cgaggaggaa gccgccgtca tcgcggaggc
    20161 gggcgacgtc ggcgccgtca cgatctcgac gaacatggcc gggcgcggcg tcgacatccg
    20221 tctcggcggg gcgcgcgagg agagggcgga cgcggttcgg gcgctcggcg gcctcttcgt
    20281 cctcgggacg aaccggcacg aggcgcgccg catcgacgac cagctgcgcg gccgcgcggg
    20341 gaggcaggga gacccgggtg aatcgcgctt cttcgtgagc ctcgaggacg acctcttcgt
    20401 gaagcacggc gcggccgact cgttgccgcg tcgcctccgg gagtgggcgg agagcggccc
    20461 cgtccgcgat ccggccgcgg gcaaggcgat cgcccacgcc cagcgcgtca tcgaggggca
    20521 ggacctcgag atccgaagga ccctgcttcg ctacgaccgg ctcctcgaga gccagcggca
    20581 gatcttcgcg gcgcggcggg acgaggcgcc caccggcttc ctcgagctcg ccgcccccga
    20641 ccggtgggtg gccgttcggg acgatttcgg cgaagagctc gcgagccgcg tcgagcgcga
    20701 cctgacgatc gcctggatcg acagcctctg gtccgatcac ctcgagaacg tcgccgagct
    20761 gcgggaggga atctcgctcc ggacgctcgg gacgcaggac cctctcgccg agtaccagcg
    20821 ggcgctcgtc gccctcttcg ccgacctcct ccgcgagctc gacgcacgag tcgccgcgac
    20881 gttcgagacg gcggagatca ccgctgacgg agtcgacctc ggccgcgagg gactcgcgag
    20941 cccttcctcc acctggacct acctggtcaa cgaccagccc ttcggctcga cgatggagag
    21001 gatggcgaag aacctggtca ggttcgcgaa ggggatgctg cggccggggt agggccgcga
    21061 cgggggccgg ttcgacgaag tgccttccgt tctgagagcg ccggtcccgc tcgccgtaca
    21121 gagccatcga aaacaaagga gcggacttga gctcctgacc gcgccagatc cgaagcctgc
    21181 gacgccagga cctccctcag ttcggcaaac gtcatcgcac ccgtcttcat cctacgcgtc
    21241 cgatcttgcg cttcccagca agtggggtcg aaccagaata cggcgtagag accgtgtgcc
    21301 tgcctcgctg tcttcatata gccgttcacc agctgcgtct ccatcgccga gagaagttcc
    21361 tgatgccagc agcctttcgc ctcaatcacg agcgagacgg tcttgctacc gctgacggcg
    21421 gcgtcgccga ctgcatcgac atagatgtcg gtcttctcgg cgccgctgag accgcccttg
    21481 atctcgacct cgcggtgcaa gatgaccgat ccccagcggc cgagccgctc gcgtagccga
    21541 ttcgcaaggt agttagttag ggcccgctcc gacttcggcc gtaggtctcc acccgcattc
    21601 tcattccaga gagcgtcgac gtccggcgtt tcaccgccga gggcgttttg gatgtccacg
    21661 catagctcga gaatcagatc cgagagctcc tgacctgtac ggaccaggca tctcgccggg
    21721 cttgcgaaca gctcgccgag ctccgtcggc gcggcaggta tccatgaacg ttcgaggtac
    21781 cgctctcgcg catccacggc gtagtaggcc aagaaggcga gctctggctg ctccacacga
    21841 atggcctcaa actctcggag agtcgccact tctcccccgc tgctgaggaa ccggaggacc
    21901 tcgtcccgaa ggtcttgcag ctcatcccgc cgggatgggg tgaacacgcc gaggtgctca
    21961 acatctccag aatacggggt attcgcgaca agccatcgat agatctcgcc cacctgccgt
    22021 gcttcgagac gctgatagaa cggcttcccg ccacggaagg tggcccgctt ggccgacagg
    22081 agcacgtcct caagcgaggc tggcgccccg accagtcgcg gccagacgag cccccagctt
    22141 tccgccggtt ccgtagaaag caagattgtc gcgcacgacg cttccttcgc gggaagcggc
    22201 ccagtcggct ccgaatccgt cagcatggcc cgactgagcg ccaccgcctc caccggcatt
    22261 tgtgccgcga gcgcttccag cagtgcctgg aaggtcgccg ggccggtctc gggcctcttc
    22321 agcacagcca caacttctgc tgtgacccac tcgtcccaga tcggtccgac gcgtgactcg
    22381 aagtcgtagc ctgcgcgcac tttgccaagg tcagaaagga gcgctctgat tgctgcgcgg
    22441 gtttcctccc gagccttcgc tagcgccagt cgccgcagct ctgagagagc ctccgagtcc
    22501 tctgtagggt aatacggaag tcctaccgtg acggctgccc acttcttcca cagctgatcg
    22561 gtggcaaggt ctgtggtggc gacgagtctc agggctgcga gtgcgaccca tgcggacatt
    22621 ggagcggagt cccgaccgag ccagacttcc tgatgcgtcg tcgcggccga gagataggct
    22681 tccgccgcac attccgcttc gcgccgaagg tcagctggga gtgcgcgcca ggttccgagc
    22741 gcccgcagat cgaacgtgag gcgaccgtga gatgaaccgc cgccttccgc gcccgcaagt
    22801 gcgtcgagaa tcgccgggaa tgtcgcttct gctccgagcc gttccctttc attcatgagg
    22861 cgagaaacta tgtcctgcgg agtcgagcgc gggagaaccg gtgctgttgt tcgctgattc
    22921 tcggaggact cccattgcgc cctcatcgcg gtcgccgtat cggacttgag cggaaccggg
    22981 ccgaacgtcc agagaatcgt ctcggagagt tccgggatcg actctcccgt ctcaaggagg
    23041 gcgtcaacgt ggtcagaaag gctggggtcg aggactcggg agatcaatcc cacgatggcc
    23101 gttctccggt cggtcccctt gctgtcccga gcgagagcga gcagttcccg caaatcggac
    23161 ggccgaagga ggggaggttc agaggcgaca agcttccacc agtggcgagg ctcgagtgtg
    23221 cggagaagat ggaaggcgat cttctgtcga cgctccgggt cctcctcgac agcttcagcc
    23281 acctccttcc cgattctttg gccactgatc agaaggtgtc cctgaaactg catcgtcaga
    23341 aagtcgacca gcgattggct gacagccgcg tcgtcggatg cacgccaggc gatggtcgca
    23401 atcggaccga ccagttgcag gagaggcgac gcctcttccg atcgcaggac tctcgccgcc
    23461 cattcgagtg ctatcgggac gtcattgacg gcgagggtcc gaatcggctg gttcctgagg
    23521 aaggacttgt atcggccata gaagttcggc cggttaggcg gcacgagggc ggcgaacagt
    23581 tccctggcgc tgataagcga gggccaaacc gtccacaggg cgaggccgcg gaactcatca
    23641 tccgggtcgt cttctcgtcc aaggttcagt agcgggacga gcacggcccg ctcttccggg
    23701 gatccgagac ctgcgaccag ctcaatggcc tcgtgcctta ggtaccgatc ctccgatggg
    23761 tccgttgcga tggggagtac cacatccacg agctcttcag ccttcgcggc gagagccatc
    23821 tcgactgcag tccgtcgcgt caacggagct cgcgtccggt cccgaatgac cgcaccgagc
    23881 gctgctccaa ttccggggtg tactaggccg tcgaagcgtg gccggatggt aggccggaca
    23941 gggttgacga gtcgcttctc gacgtgggtc agatacgaga gcgcgagtgc gtcctttcgt
    24001 tccggaggta ccgagtctcc accaagggtc agcagcacct caggatcgga ctcgacgaag
    24061 gcctcgaaga actcgcgctt ctgtgatgct agccaagctg cgacgccgcg caactgcggg
    24121 acgacccgcc gtgcgtgatc cggatctgtt gatcgcaaga gcctgagcat ctgcgtggtg
    24181 gggatgcctg aagagagcag gtaccgagca cagaggaact gcgcatgggc ttgctgcgcc
    24241 cagccaagca gcccctggcc gcgcgacgtg aagaggcccg aacccgcgag ttcgcggatg
    24301 aatgtctcgg agatctgaac gatcgggccc ccgccgagac tcatcgcacc ccgcgcgaga
    24361 tccgctacgg atagatctgc ggcggggtcc agtcgtgacg agcctgcctg cgcgaatacc
    24421 atccggcccg atagcagcat catggctgct atccgccccg caagatctat tcgttgctcg
    24481 acaccgagct ccccggcggc ctccgtctcg atccgctcgt ccacctctct gcagagttcc
    24541 ctgcagcccc gctcgtagat gtcccaaggc gcggacggga gtctgccttg agtccggaat
    24601 aggggcgtgt cccgggcgct gttgaatttc gaatgaagag gggcggctcc ttgccgg
//
LOCUS       DAOONL010000050        24655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1905_length_24655_cov_17.820163, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000050 DAOONL010000000
VERSION     DAOONL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_24655_cov_17.820163"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLP50_09245"
     CDS             <1..1072
                     /locus_tag="PLP50_09245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPA51776.1"
                     /translation="AESPKTASNQKKIREFVDAHGIARFHDVRGDVGGICHQILPEYG
                     YVRPGQVVVGTDSHTTSHGALGAFSFGIGATEMASVWTLGVALNVEVPPTIKVEVTGE
                     FPPHVGPKDLILHLVGKLTAQGANFRVLEYHGETMRKMSTSGRLAVCNMSVEAGATSG
                     IVPADEETLRYLREEAGVTDPIELVVPDPDAVYERVVTVDVSRMEPQIACPHTVDNVK
                     PISEVAGVKVHQVVIGSCTNGRIDDLEAAAAVVRGKKVAKGTRMLVFPASGRVFQEAL
                     DRGYVADFMKAGAVVMNAGCGPCLGVHEGALGDNEVAVSTTNRNFKGRMGNPKSEVYL
                     CSPAVAAASAVAGVLADPRKGS"
     gene            1075..1575
                     /gene="leuD"
                     /locus_tag="PLP50_09250"
     CDS             1075..1575
                     /gene="leuD"
                     /locus_tag="PLP50_09250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPA51777.1"
                     /translation="MGKVAFVVGDDVSTDVIYPGRYMATVLPTETPQFAFANDPALNA
                     KLNAKEVPPGSVLVGGKNFGCGSSREQAVSCLKGWELTIVASDVARIFLQNSINLGLN
                     IVTAPGVEASVGDEVVVEGKKVVNRTTGKSWDVVPLPPARQAIIDAGGLIAYTRKKML
                     EARARG"
     gene            complement(1760..2608)
                     /locus_tag="PLP50_09255"
     CDS             complement(1760..2608)
                     /locus_tag="PLP50_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51778.1"
                     /translation="MAVAFLLRSLASAALLGAASLAHAELPAAGGLVVSPTRVVLVGD
                     GAGAKLVVRNTRASTTTYRLSFVRMRMGERGAMSFVTEAWTGELFADGLVRFSPAVLK
                     LAPGLDHAVRLKVRLPHGVGPGEYRSHLLFRPVEAPGEADAVAREEGGAAGDARAGPP
                     VGVTVPVIVRVGDVSAEVRIASLTVRGTAEGSRLEVVLERSGSASIYGDLAVRLESDG
                     ARTELVGGLAGLAVYAPNERRVVEIPLRLPEAMPAGEPRLRVEYRSAGEDGALLAVRE
                     AALPPR"
     gene            complement(2632..3003)
                     /locus_tag="PLP50_09260"
     CDS             complement(2632..3003)
                     /locus_tag="PLP50_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51779.1"
                     /translation="MRRSRRTESQPIRRTVRFAAGGLLLLLCLAFSASAAAAAGNTSV
                     GIRITSWPVGPSQAHVTAVTDSEMPAEVAATVSTSRPIAASTPAGTKKAAAASATPSM
                     ISADRLPGESDERALVLVSWE"
     gene            complement(3143..5770)
                     /locus_tag="PLP50_09265"
     CDS             complement(3143..5770)
                     /locus_tag="PLP50_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA51780.1"
                     /translation="MPPPGGAAGTRVDAGPAGRSATLTVRLRALALVLLLAFTPPALA
                     ARALEVSGETAVVAGIVVNGQEVATEELIRAEGTLWIPLGPLAEWTYSEVRTVESQVQ
                     LVTPLGMALVNDEELREFDGQRYVSVAFLERRLGARVAFDEAQGALVVQLAWSEPAAA
                     TGPGPRLTPDVRAPGSSLSLLRQDLYYDRSHSDGRATSATTVAGRVAGGRFRLRLDSD
                     LENYSLLSEAVWVKDLGRAKVLVGHQNVSLNPLLPSSSLSGAQLAWTNKPETLFPVST
                     DGRELLPRQMRSTTTIRGQGPIGGIAELRVDGVFRQRVTVGLSGIYEFLDVELPGRAS
                     TRVEVYTFSRDNLLVPTGVQAKTVATSDYLLPAGTWTLVGGAGAAGNLGSELLGSDRG
                     GKGAIGFVQARRGISKSLGVEVSARADEEGWQVVGGLDAVLREAVTLSLFAGTGKDGS
                     GYRGEAGWFTPRVRLSAFSSMRDEGFAGPASPRNEEHYGEALVRPLPWLEVGAAARSW
                     RSADAGFDWVRPVLSARPAPWLMVRAWPVFDGEYRVDLFAQMGRKTTLSAYHQGYFGA
                     EASSALDSRHRVSVGAELGGDRAPRVSVVARRQGVRSFRADLGLGAIWSDGKVGVLAS
                     AFYPLTAGLFLRGEYESVQLSRAKGTPSSPRVLVGLSANFGYVGNRFLPTDSSPWSTG
                     RGALAGRIRREAGDAEVNADLAGIAVRVDDRVAARTLAGGTFYVGDLSEGVHRVELDD
                     ERLPIELVPVRNELVVEVVAGAVTRVDFPVRVELSIAGRITCAAGAPARGAIIEAVDG
                     DGRVVGSTESDSFGLYRLDGIRPGSYSVRVAAEGAQGPAATRAVTVTNDFLFDVDVKV
                     DCPVGTLPPAGVAPAQNDR"
     gene            complement(5701..6591)
                     /locus_tag="PLP50_09270"
     CDS             complement(5701..6591)
                     /locus_tag="PLP50_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51781.1"
                     /translation="MTALRRLSIAALIGLGFLAPGARAQIALSPPIVEVDLSGRSATE
                     SVRLINMGKRDVEVSVRVEPFDLDEANKYRSLPSTEDSLDRWMVVQPGRFVVPAGQSR
                     AVRFSIRPKSRPALGEHRAMLFFEEVERESGPATTLKALFRVGAAIYAHVGEVRRVGR
                     VVSTSCSRDGGLLEIASEGSANARMTGRWGLWSASAFPGIGATTYSTAAASAAEGARP
                     TPPAGLLFEGSLPTVPVLPGTRRNVLVPLPKEAPEGDLVLHLLGTLGGTPLDLAVPCR
                     LPAAPPAPESTPAPPAAPPR"
     gene            complement(6588..7175)
                     /locus_tag="PLP50_09275"
     CDS             complement(6588..7175)
                     /locus_tag="PLP50_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51782.1"
                     /translation="MWAERGRLPSIGLRLAGAALVSLCTWPAAGVPPAPPRPAVGFQI
                     ELPRLVELDSIGGRDLLATGNAPTNGLVTGLSFDLGSYSAAGASRPHALRARVVRRVE
                     KLWRVALEGSTKESDAHEVRVELIAPDGTVGALGVAGEPAAKIAIRAVPLNPVRIEEG
                     GTAFLQGGVELELDLATARQAGRYEGSISVTVIPR"
     gene            complement(7206..7805)
                     /locus_tag="PLP50_09280"
     CDS             complement(7206..7805)
                     /locus_tag="PLP50_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51783.1"
                     /translation="MKNSMKLLALVLVAAAALFGAVQPAQAQAQGTTAVSVNVNGIVI
                     LYYYSSVNVTMPADLLGSQAVSAGAAAPTAVAVATGAEATLANPTSATPVSLAAMNLD
                     ILNAWSVRSLNTASGTTQVTISGTQDTLTAGAGNVITIDDRQVRATSGAFGATSTFPS
                     AGTFVTPQTGDVRLVLDMSATTATGNFAGGNYTLTAVNL"
     gene            8329..10722
                     /locus_tag="PLP50_09285"
     CDS             8329..10722
                     /locus_tag="PLP50_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HPA51784.1"
                     /translation="MAPAADRNWLLAALGCLALSASLAPPAVSQAIANGSFETGNLSG
                     WTVGGVGRADAVRADDFDRPFYDFDLPFPDGTWAALLSTNSTANASSTDLDGNGVPEY
                     HVTTLSQTFSVSAAPVNLSLSWLVLTDERRRTGGGAAAYDDLFSIDLSGTPILRRSVY
                     RPGGASPWTDTPAYNGIRYRVSKGGSPVNGSDFQGTNGGGRMTAFSDLCVTISQPGTY
                     TLTFTVADQGGTDSSYDSALMVDKVELPSSCVASPIRMTDTSGANVEVKSGGVVFNPH
                     FNRQPATSDDGSVIAFVSNANLTSDNPNAVEQVFTFASNVYRRLTSFASGTVGRPSIT
                     RNGRFVAFASNAPAAGNPDGNSEIYRYDTVTSTLTQVTNTTGCTNGAPSISDDPAGSS
                     IFFETTCNLGGANSNGNREIARWTGDSTFLVTAGSPTACTSHSPHAAWGTPTTAVFVS
                     TCGFTGAANADGNAEIFRWVAPSSPGTYTRITNTTGAIASDAPSITADGAFVSFVSNA
                     NLASSNADGSFEVFRWQSGNSSFVQATNDASGLVAYTSARIGGSNGNLIATERLSLLS
                     GTFETSLLTVGGGEAVLLTSSDFTLPAVAAGASPPAVVFQSSADLRGENPDRNLEIWA
                     SDSSVGRVRTYTRTLPPLAILQCGGYSQICECTNPRTTTDDLVVSDPGTVGSLVVSLV
                     VTHTYVGDLIFRLTHVESGTTVTLLHRPLVPDPNPTAQCGCSNDNLNVVFDDAAATAA
                     DGQCSGNPAINGRFRPYTPLSAFAGAPLAGTWRLTAVDMAGQDTGSISSWGMTVTLQ"
     gene            10734..12530
                     /locus_tag="PLP50_09290"
     CDS             10734..12530
                     /locus_tag="PLP50_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HPA51785.1"
                     /translation="MRTTLRHAFLLFAPAVIALFAPTAPGQALPEPIAPPDTWGYEMY
                     DQTTGSCPFQFVDLASGGSLLTFPAPGDDEGAVLVPTRPFQLYGSPVFGFVASTNGYL
                     AATGSLSAEDGRDYSNDSVPSVPGYVPLVPGTPTASFPGRIAVFHDDLALGGGSVRWQ
                     EAASCPRASGAVAGEACTVVQWSGVSRAGGSEPLTFQAILYHSSFAIALQVAASDGSG
                     GGSASAGIQDLEARLGLSYAANEPGRLGPGTAVCFFDPRHPAGGPRADLRLSTTAKVE
                     PSAPDALVTYGVAVFNAGPSDATGGTVDAPFPSGLEWESDSCGGAFAAGQWVLDALPA
                     GGSAECHVTARVVATVEGIVTHPFVVNGDATDPDPTNDGVDVLVQVFVPAPVDDGDGV
                     PASTEDSFPGGLGVPPLAPGDGNGDGIADSLQAGVASLPAASGAGWLTVELLGGGCSS
                     LQEVAALAESAVAEGDLQYDFPHGLVAFRIPCPAGGASVRVWYHGASAVPPVYRKYGP
                     LVPGDGVAVWYTLPGVGFGESRGIPTASFTLVDGQTGDDTGIDGVIVDQGGPGQPGES
                     VATIPVAGPWGLATLAALILAAGIALQRSRLR"
     gene            12733..13239
                     /locus_tag="PLP50_09295"
     CDS             12733..13239
                     /locus_tag="PLP50_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51786.1"
                     /translation="MTKHAAARSSRATTKPRSAWAFPAIPLALAVAILAGQPAVAATS
                     SVEVSAFIRATASCSVDLQPGSVRVSADDVRRGYVDLPASSRVRVRTNSSNGYMLVLE
                     VESSEFDAVVLEESGERVEIRGTGFVERPYRGRAEVVSELGYRLLLNPATQPGVYAWP
                     VSLSAQPR"
     gene            complement(13331..13795)
                     /locus_tag="PLP50_09300"
     CDS             complement(13331..13795)
                     /locus_tag="PLP50_09300"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HPA51787.1"
                     /translation="MRPRLLLAFFAAGAAIVGAPARADIRCSIQNVTPVSFSTYDVFR
                     TSPRDAQGSVTYTCRGVGQRTVTIDLSSGSSGRFNPRSMSSTGSTLPYNLFLDSACTT
                     IWGDGSGGSSRYGPLSPPNNTSIVVPIYGRIPPEQNVAPGSYADSVTLTLNF"
     gene            complement(13834..16176)
                     /locus_tag="PLP50_09305"
     CDS             complement(13834..16176)
                     /locus_tag="PLP50_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HPA51788.1"
                     /translation="MRAERLPGCLILALLLAWSAAARAGETIVVRVVLNEIPKGELFA
                     VLEDDGGILLKAEDLTALGLVPADRPVRTIDGEPWVALGEAAGITFRLDMATLTLHLV
                     ATPASFPSSTLDLLPKRRLDAIRTRDRSAFFNYRLDYTGEAGLRLRNLSFGQELGVRL
                     GDWLLHGEGRWERTDEQQRWLRLSTSLTRDDRERLTRFVAGDFVGTSGLLGSALGLGG
                     VSYSRAFRIDPYLVTRPAFEFTGSALTPSQVDVFLDGQKIATQAFPPGTFSLRNIVGY
                     TGARTLEVVIRDVAGREERHLVPFYFSDVGLSRGLSDFSYSIGAPRREFGQASFDYGS
                     LTFLGFHRWGVTDGLTLGARAEANGDVFNAGPDVAAVLGRWGTLTAAANVSAGRGAET
                     GWAGALSWVYGGRSLGGRLFVRSFSSGYATVDEASSSARTRVETGVGLNATSPRLGSL
                     AFDLSRRERFDGPTESSVSTTFFRRVWRSAHLRVSGRWTSGESSRRELFVGLTAYLGG
                     QATLSAGARSENGRESFAVEGLRGAPLGEGIGYGLSARVPLEGGGETQGSANVQVKRR
                     FGLFTAGVDLSDAPGAGPSETYRLSAAGGLAWAGGALRLTRPITDSFGVARVGTLEGV
                     RVSQNGQVVGRTDDTGIVFLPELASFLENQVTIENRDIPLDYSVPALTKVVSPPFRSG
                     SVLSFDVQRVQGVTGTILARREGDVVPVEFAVFTLETAAGPVEHTTGTGGEFYLEDLA
                     PGRYVGRASPAGRPCTFTLEVPDSTEFIFEAPPLVCEPNR"
     gene            complement(16166..16915)
                     /locus_tag="PLP50_09310"
     CDS             complement(16166..16915)
                     /locus_tag="PLP50_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HPA51789.1"
                     /translation="MKAGWTALPSLALTAAFVLARPASGAEFSVSPIRIEFDRTTRTS
                     SVNVRNDGDRPLRAQVTLSEWSQDGDGRDVYSDSKDLVFFPKILSVEPGAEAVVRIGV
                     RSLPASVEKTYRLYVEEMPEPSPEGGARVNVRVRFGVGVFVQPPVKSTRAEIVALDVR
                     DGTVHVPVRNTGSVSFRVDKVLLCGTRASGEPLSREIGGWYVLAGATRPFQARLTEEE
                     CRGLVRIDVAAVGADLKQEAGLDVPDGACAR"
     gene            complement(16947..17429)
                     /locus_tag="PLP50_09315"
     CDS             complement(16947..17429)
                     /locus_tag="PLP50_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HPA51790.1"
                     /translation="MLRRRLLRVLVVLVCGAMSHGAVADGGLVSVSATVLSKSNCKFR
                     TSAAALPFGTLDAALSSDRVVSATLDFRCQGSAPIASFMFSSDDGLNEFGPNRPRMKH
                     ATDSNEYLPYSLGLSPATGTIGKGVTGTLTLTGTVTPADVAASIAGSFSDTVTLTLLP
                     "
     gene            complement(17541..18020)
                     /locus_tag="PLP50_09320"
     CDS             complement(17541..18020)
                     /locus_tag="PLP50_09320"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HPA51791.1"
                     /translation="MKKTALIALTALAAIAVLVPAGNLMANDSATVTVTANVVGTCKF
                     VSPKTGAVSFGALDPSAATDVAGTVTQPQFWCTKGAAYTISDDNGLNETGTTFRMVHG
                     TNAAEFIPYSFTYTASGTGTGPQSPFTMDIAAQVLGASYANALAGAYSDTVTLTINP"
     gene            complement(18191..18490)
                     /locus_tag="PLP50_09325"
     CDS             complement(18191..18490)
                     /locus_tag="PLP50_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51792.1"
                     /translation="MTTFGLVLVVGVPLLVLVVYLEGKRQARIYGPPSARPNLAGAGL
                     LELQKHLQADRRVDTLVEQVRDEAAETEQDAGGEGRRPSADTGREPRPAPPDRKS"
     gene            18539..19444
                     /locus_tag="PLP50_09330"
     CDS             18539..19444
                     /locus_tag="PLP50_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HPA51793.1"
                     /translation="MSEPPTVPSPGVERPRRSVLYVPGSNAKALAKARTLPCDAVILD
                     LEDSVAPEAKEPARRAVVEAVAAGGFAPRELLVRVNARGTPWHREDLAALARLPVDGI
                     VLPKVETLEDVREAAGVLGASGATAAPALWLTIETPRGVLAADLLAGAHPSVAGLVAG
                     TSDLTKELRARHVPGREPLLHALSAIVLAARAHSLAALDGVHLALEDEEGFAAACRQG
                     RDLGFDGKTLIHPRTIEAANRAFAPDPSEVEAARRILAAWEAARAEDRGVVVHDGHLV
                     EELHAREAERLVALAGAIASRTAGG"
     gene            complement(19457..20014)
                     /locus_tag="PLP50_09335"
     CDS             complement(19457..20014)
                     /locus_tag="PLP50_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51794.1"
                     /translation="MNCRKSAPVLLAILVLTSCASGGGTDRALVEAGRKLRREQPPPT
                     AAELRLEVDSFLDNELPLLRDKATKRIEREQRIWRAWFVGGAALGVLAASSGSVAGSE
                     GATKALLVGAGGVGTLVGGVGYYTRAPKLEACRVFLAAARADVASWRANAIPPGDGPV
                     PPAVWHAWVDRVAAIRAAGPCATVR"
     gene            20079..20996
                     /locus_tag="PLP50_09340"
     CDS             20079..20996
                     /locus_tag="PLP50_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA51795.1"
                     /translation="MSRVLSLHDVVKHYGSVKAVDGVSFEVNAGRITGLLGRNGAGKT
                     TTLRMITGVLRPDRGRVELFSAEVEAARDRLGYLPEERGLYRKMRVLDHLLFLAEIKG
                     RSPAAMRPAAERWLTRFELQEKRNAKVEELSKGNQQKVQLAGALLFDPELVVLDEPGS
                     GLDPVNVVLVRRLLRELAAEGKAILLSTHQMGEAEKLCDEIVLVHAGKVVRSGPLAEV
                     KASGGRDAVHLEFAGDGAFLAELPAVASARVDTNRAELRLAPGGDAQEVLAAAAARLR
                     VLRFEVVAPSLEEVFLEAVGETAPPEVAA"
     gene            20993..22318
                     /locus_tag="PLP50_09345"
     CDS             20993..22318
                     /locus_tag="PLP50_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51796.1"
                     /translation="MRKLWAVLKREYRETVRKPSFLIMTVLAPFLFGALMLVPALLAT
                     RGMGERKVAVLDGTGRLRAAVEGLSGEAPGERSAFARGRGTNVPTGRIVPEYVDLFTV
                     DPKNAVGPYLERLSVERTPKEQRLDGVLLVPADAFERPLTHLTYFSRSAADLLAQERL
                     GRAVNRALQRERLAARGVSPAELELILAPLPVETVQVTKSGQERRGGEGSFVLAMLFL
                     ALLFIPSLVYGQEVMRGVIQEKTDRVVELLVSSMTPMELLSGKILGMAAIGLTQMAVW
                     VTMGGSLAVAGLSEARSAGFDLSTVLRPSIAAWFVVFFLLSYLVTVGVYAAGGAIAGS
                     EKEAQQVLTPVMFVFLVPWFLMMPILTNPDSTLSVVLSLVPIYTPMTMFIRLLVSEPP
                     AWQVALSLVLSVATIAGLMKASAKIFRAGLLATGKRPTIPEIWRWLKEA"
     gene            22545..22781
                     /locus_tag="PLP50_09350"
     CDS             22545..22781
                     /locus_tag="PLP50_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51797.1"
                     /translation="MQPTDSEILWLNLTNAGLGLATLGGIVALGWAAVRELRERAKGR
                     VAVHDLDPHAAFIPELGLTMADGGEKVDENEEKK"
     gene            22807..23754
                     /locus_tag="PLP50_09355"
     CDS             22807..23754
                     /locus_tag="PLP50_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51798.1"
                     /translation="MRCPFLREAHVQSCQASPIRKQIVRTGSASDERCATAAWHECPV
                     VRELNEEHPSASSCPFRTESLAQYCSAAPVTKLIPWSDQAFSRCGSEAHRYCDLYLAL
                     ARTPAPTAAPGEAPPRSDDADGIPVPQWLWFAPGHTWLSVASDGVCHIGVDAFLARTL
                     GGIDRVQFVTTKGLERPAAVLTVRDVDVTVSFPNPVFITAVNGALRADPARLARDPYG
                     LGWLFEGIVRDRSAVPNGAPRITDGLLRGEAATSWMRRETLRLRGLAEEAAERISGVR
                     LAADGGLPEPALVRTLPHEEALRLSHLLLSTASDEERST"
     gene            23751..24329
                     /locus_tag="PLP50_09360"
     CDS             23751..24329
                     /locus_tag="PLP50_09360"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPA51799.1"
                     /translation="MKLPALVSITAGLVPALAAGWLLFPRLIYKTEAQPLPFNHKVHA
                     EQGMSCGDCHAVAEDGRFAGMPTTESCAGCHAEKGENAGVNALVERYVEPGVEIPWLS
                     NARQPDNAYFPHAAHVTGEKVACARCHGPHGESTAVRPVQVNRLSGYPRDIWGPSISR
                     LRREEWQGMKMSDCIACHRAGGRESACIDCHK"
     gene            24340..>24655
                     /locus_tag="PLP50_09365"
     CDS             24340..>24655
                     /locus_tag="PLP50_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HPA51800.1"
                     /translation="MKLEVSRRDLLLAGTGAAAGIALSPVPWKALDDLSIWTQRPPTP
                     TPRPAGPLAFRFTSCTLCPAACGLKARSFGPQPVGFSGVPGHPASDGGLCPLGLTAHH
                     LAK"
BASE COUNT         3595 a         9144 c         8423 g         3493 t
ORIGIN      
        1 ggccgagtcg ccgaagaccg cgtcgaacca gaagaagatc cgcgagttcg tcgacgccca
       61 cggcatcgcc cgcttccacg acgtccgcgg cgacgtcggc ggcatctgcc accagatcct
      121 ccctgagtac ggctacgtgc ggcccggaca ggtcgtcgtc ggcaccgatt cgcacacgac
      181 gagccacggc gccctcggcg cgttctcgtt cgggatcggc gcgacggaga tggcctccgt
      241 ctggacgctc ggcgtcgccc tcaacgtcga ggtcccgccg acgatcaagg tcgaggtgac
      301 gggcgagttc ccgccgcacg tcggcccgaa ggacctgatc ctccacctcg tcggcaagct
      361 caccgcccag ggggcgaact tccgcgtcct cgagtaccac ggcgagacga tgcggaagat
      421 gtcgacctcc gggcggctcg ccgtctgcaa catgtcggtc gaggcgggcg ccacgtcggg
      481 catcgtcccg gccgacgagg agacgctccg ttacctccgc gaggaggcgg gcgtcaccga
      541 cccgatcgag ctcgtcgtcc ccgacccgga cgccgtctac gagcgggtcg tgacggtgga
      601 cgtgtcgcgg atggagccgc agatcgcctg cccgcacacg gtcgacaacg tcaagccgat
      661 ctccgaggtc gcgggggtga aggtccacca ggtcgtcatc ggctcctgca cgaacgggcg
      721 gatcgacgac ctcgaggcgg ctgccgcggt cgtccgcggg aagaaggtcg cgaaggggac
      781 ccggatgctc gtcttccccg cgtcggggcg cgtcttccag gaggcgctcg accgcggcta
      841 cgtcgccgac ttcatgaagg cgggcgccgt cgtcatgaac gccggctgcg gaccgtgcct
      901 cggcgtccac gagggggcgc tcggcgacaa cgaagtcgcc gtctccacga cgaaccggaa
      961 cttcaagggg cggatgggga acccgaagtc ggaggtctac ctctgctcgc ccgccgtcgc
     1021 ggccgcgtcg gccgtcgccg gcgtcctcgc cgacccgcgg aaggggagct gaacatgggc
     1081 aaggtcgcat tcgtcgtcgg ggacgacgtc tccaccgacg tcatctaccc gggccgctac
     1141 atggccaccg tcctcccgac cgagacgccg cagttcgcgt tcgcgaacga cccggcgctc
     1201 aacgcgaagc tgaacgcgaa ggaggtcccg cccggctccg tcctcgtcgg cgggaagaac
     1261 ttcggctgcg gctcctcgcg cgagcaggcc gtctcctgcc tcaaggggtg ggagctgacg
     1321 atcgtcgcga gcgacgtcgc gcggatcttc ctgcagaact cgatcaacct cggcctgaac
     1381 atcgtcacgg ccccgggcgt cgaggcctcc gtcggcgacg aggtcgtcgt cgaggggaag
     1441 aaggtcgtca accggacgac cggcaagtcg tgggacgtcg tcccgctccc gccggcccgc
     1501 caggcgatca tcgacgccgg cggcctcatc gcctacacgc ggaagaagat gctggaggcg
     1561 cgggcgaggg gctgactctc ctcacgacgc ccgggtcgcg acgacggggc gtcgtctcgg
     1621 tcgatgtccg ttccgagccc cgcccgtcgc tctcgggacg tcccggcggg cctcgcgccg
     1681 gtggagcgcc tgcgcggtgg gcgactcgcg cgatgtcggg aggcgctccg tgcggcctcc
     1741 gcggacgcgt tgccggactc tatcggggag ggagcgccgc ctctcgcacg gcgagcaacg
     1801 caccgtcctc tcccgcggag cggtactcga cccgcagccg tggctcgccg gccggcatcg
     1861 cctccggcag ccggagcgga atctccacga cacgccgttc gttcggggca tagacggcca
     1921 ggcctgcgag gcctcccacc agctcggtac gggctccgtc ggactccagc cggacggcga
     1981 gatcgccgta gatggaagca ctcccgctgc gctccagaac cacctcgagg cgggagccct
     2041 ccgcggtccc gcggaccgtg aggctcgcga tgcgcacctc cgcgctcacg tcgccgactc
     2101 gtacgatgac cggcacggtg acgccgacgg gcggcccggc gcgagcgtcg ccggcggcgc
     2161 ctccctcttc ccgagcgacg gcgtccgctt cgcccggcgc ttccacgggt cgaaagagca
     2221 ggtgggaccg atactcgccc ggcccgacgc cgtgcggcag cctcaccttc agccgaaccg
     2281 cgtggtcgag accgggggcg agcttcagca cggcgggaga gaagcggacc aggccgtcgg
     2341 cgaagagctc gccggtccac gcttcggtca cgaagctcat cgcgccgcgc tctcccatcc
     2401 tcatccgaac gaacgagagc cgatatgtcg tcgtgctcgc tctcgtgttc cggacgacca
     2461 gcttcgcgcc cgccccgtct cccacgagga cgaccctcgt gggggagacg acgagcccgc
     2521 cggcggcggg gagctccgcg tgagcgagcg acgccgcgcc gaggagcgcg gcagaggcga
     2581 ggctacgaag gaggaacgcg acggccacct ggccgggatc gtagctcctc gctactccca
     2641 ggagacgaga accagggcgc gctcgtcgga ctcgccggga agacggtccg cggagatcat
     2701 cgacggcgtc gcggaggccg ccgcggcttt cttcgtccca gccggagtgg acgcggcgat
     2761 cgggcggctc gtgctcaccg tcgcggccac ctcggcgggc atctccgaat ccgtcacggc
     2821 cgtgacgtgc gcctgcgacg ggccaaccgg ccacgaggtg atccggatcc ccacgctcgt
     2881 gttccccgcg gccgccgccg ccgaagcgga gaacgcgagg cagaggagaa gaaggaggcc
     2941 gccggcagcg aatcgaacgg ttcggcggat cggctgcgac tcggttcggc ggctgcggcg
     3001 catgccggga catgcgcaag gctgcggcca gccctaggca ccgcgtaaac agcttacaga
     3061 caactatttg aacgaacgag accggagggg gacttgtgtc atgagacatg gtggggctgt
     3121 gtcccggtac acgccccgac cgtcagcgat cgttctgcgc gggcgcgact cccgccggcg
     3181 gcagcgtccc gaccgggcag tccaccttga cgtctacgtc gaagaggaag tcgttcgtca
     3241 ccgtgaccgc gcgagtcgcc gctgggccct gcgcgccttc ggccgcgacc ctgaccgagt
     3301 agctcccggg acggatcccg tcgaggcggt acaggccgaa tgagtcggac tcggtactcc
     3361 ccacgacacg tccgtcgccg tcgaccgcct cgatgatcgc cccccgcgcc ggcgcgcccg
     3421 ccgcgcacgt gatcctgccg gcgatcgaca gctccacgcg aaccgggaag tcgactcgag
     3481 tcacggcgcc ggcgacgacc tccacgacga gctcgttcct gacgggcacg agctcgatcg
     3541 gcaagcgctc gtcgtccagc tccacgcggt ggacgccctc ggacaggtcg ccgacgtaga
     3601 acgtcccgcc ggcaagggtc cgtgccgcga cccggtcatc caccctcaca gcgattccgg
     3661 ccaggtcggc gttcacctca gcgtcgccag cctcccgtcg aatccgcccc gcgagcgcgc
     3721 cgcgtcccgt ggaccagggg ctcgagtcgg tcgggaggaa gcggttcccc acgtatccga
     3781 agttcgccga aagccccacg aggacgcgcg gcgagctcgg cgtccccttc gcccgcgaga
     3841 gctgcaccga ctcgtattcg ccgcgcagga agagcccggc ggtgaggggg tagaaggcgc
     3901 tcgccaggac gcccaccttc ccgtcgctcc agatcgctcc gaggccgagg tcggcccgga
     3961 agctccggac gccttgccgg cgggcgacga ccgagacccg gggagcccgg tctcccccga
     4021 gctccgcacc caccgaaacg cgatggcgcg aatcgagggc ggaggacgcc tcggcgccga
     4081 agtagccctg gtggtaggcc gagagggtgg tcttccttcc catctgagcg aagaggtcga
     4141 cccggtattc gccgtcgaac accggccagg cccgcaccat gagccacgga gcgggccggg
     4201 cggagaggac cggccgcacc cagtcgaacc cggcgtccgc gctcctccag gatcgcgcgg
     4261 ccgcccccac ctcgagccag gggagcgggc gaacgagcgc ctcgccgtag tgctcctcgt
     4321 ttcgcggcga cgccggtccc gcgaacccct cgtcgcgcat ggacgagaag gccgacaggc
     4381 gaacccgcgg cgtgaaccag cccgcctcgc cccggtagcc gctcccgtcc ttccccgttc
     4441 cggcgaagag cgagagagtc accgcctcac gcaggacggc gtcgaggccg cccacgacct
     4501 gccagccctc ttcgtccgct cgcgccgaga cttcgacccc gaggctcttc gagatccccc
     4561 gccgcgcctg gacgaacccg atcgcccctt tccccccgcg gtcgctgccg agaagctccg
     4621 acccgaggtt cccggcggct cccgctcccc cgacgagcgt ccaggtgccc gccggcagca
     4681 ggtagtcgga cgtcgcgacg gtcttcgcct ggacaccggt cgggacgagg aggttgtcgc
     4741 ggctgaaggt gtagacctcc acccgcgtac tcgcccggcc tggcagctcc acgtcgagga
     4801 actcgtagat ccccgaaagg cccaccgtca ccctctggcg gaagacgccg tccacccgga
     4861 gctcggcgat gccgccgatc ggtccctgcc cgcggatcgt cgtcgtcgac cgcatctgcc
     4921 gcggcagcag ctcgcgaccg tctgtcgaga ccgggaagag ggtctcgggc ttgttcgtcc
     4981 aggcgagctg tgcgcccgac aggctcgagg aggggaggag cgggttcagc gacacgttct
     5041 ggtgccccac caggaccttc gcccggccga ggtccttgac ccagaccgcc tcggagagga
     5101 gcgagtagtt ctcgaggtcc gagtcgaggc gcagccggaa gcggcctccc gccacccgcc
     5161 cggcgaccgt cgtcgccgag gtggcgcgcc cgtccgagtg cgaccggtcg tagtagaggt
     5221 cctgccggag aagggaaagg gagctcccgg gcgccctcac gtcgggcgtg agcctcggac
     5281 cgggacccgt cgcggccgcc ggctcgctcc aggccagctg caccaccagc gcgccctggg
     5341 cctcgtcgaa ggcgacccgt gccccgagcc gtctctcgag gaacgcgacg ctcacgtagc
     5401 gctgcccgtc gaactcgcgc agctcctcgt cgttgacgag agccatgccg agaggcgtca
     5461 cgagctgcac ctgcgactcc accgtccgaa cctcggagta ggtccattcg gccagcggcc
     5521 cgagcgggat ccagagcgtc ccttccgcgc ggatcagctc ctccgtcgcg acctcctgcc
     5581 cgttcaccac gatccccgcg acgacggcgg tctcgccgct cacctccagc gcgcgcgcgg
     5641 ccagagccgg cggggtgaag gcgagaagga ggacgagggc gagcgcccgg agccgcacgg
     5701 tcaacgtggc ggagcggccg gcggggccgg cgtcgactcg ggtgccggcg gcgccgccgg
     5761 gaggcggcac ggcaccgcga ggtcgagagg cgtcccgccg agcgtcccga ggaggtgaag
     5821 cacgagatcg ccctccggcg cctccttcgg aagcgggacg aggacgttcc tccgcgtgcc
     5881 ggggaggacg ggcaccgtcg gcaggctccc ttcgaagagg agcccggccg gcggcgtggg
     5941 ccgcgccccc tccgcagcgc tcgcggccgc tgtcgagtag gtcgtcgccc cgattccggg
     6001 gaaggcggac gcgctccaga gaccccatcg ccccgtcatc cgcgcattcg cgctcccctc
     6061 gctcgcgatc tcgaggagcc ccccatcgcg cgagcagctc gtcgaaacga cgcgcccgac
     6121 gcgccggacc tcgccgacgt gcgcgtagat cgccgcgccg acgcggaaga gcgccttcag
     6181 cgtcgtcgcg ggcccgctct cgcgctcgac ctcctcgaag aagagcatcg cgcggtgctc
     6241 cccgagcgcc ggcctcgact tcggccggat ggagaagcgg acggcccggg actgtccggc
     6301 cggcacgacg aagcgcccgg gctggacgac catccaccgg tcgagcgagt cctcggtcga
     6361 agggagcgac cgatatttgt tcgcctcgtc gaggtcgaac ggctcgacgc ggacgctcac
     6421 ctcgacgtcg cgcttcccca tgttgatgag gcgcaccgac tccgtcgccg agcggccgga
     6481 gaggtcgacc tccacgatcg ggggcgagag ggcgatctgc gcccgggcgc ccggagccag
     6541 gaagccgagc ccgatgaggg cggcgatcga gaggcgccgg agggccgtca tcgcggaatg
     6601 accgtgacgg agatggaccc ctcgtagcgg ccggcctgcc tggccgtggc gaggtcgagc
     6661 tcgagctcca cgcctccctg caggaacgcc gtgcccccct cctcgatccg gacggggttc
     6721 agcggaacgg ccctgatcgc gatcttcgcc gcgggttctc cggcgacccc gagcgcgccg
     6781 accgtcccgt cgggagcgat cagctcgacg cggacctcgt gtgcgtcgga ttccttcgtc
     6841 gagccctcca gcgcgacccg ccagagcttc tcgacgcgcc ggacgacgcg cgcccgaagg
     6901 gcatgggggc ggctcgcgcc cgcggcactg tagcttccga ggtcgaagga gaggcccgtg
     6961 accaggccgt tcgtcggggc gtttccggtc gcgaggaggt cccgtccgcc gatcgagtcg
     7021 agctcgacga gacgaggaag ctcgatctgg aaaccgacgg ccggccgggg gggcgccggt
     7081 ggcacccccg cggccggcca agtgcagaga gagacgagcg cggcgccggc gagccgcagg
     7141 ccgatcgagg gaagcctccc gcgctcggcc cacatcgtcg tcggcgcccc cctcctcgat
     7201 ccgcgctaca ggttgacggc cgtgagggtg tagttgccgc cggcgaagtt gccggtcgca
     7261 gtcgtcgccg acatgtcgag cacgagccgg acgtcaccag tctgaggagt gacgaacgtc
     7321 cccgccgacg ggaaggtgga ggtcgccccg aaggccccgg aggtcgcgcg gacctgcctg
     7381 tcgtcgatcg tgatcacgtt cccggcgccc gccgtgagcg tgtcttgcgt tccgctgatc
     7441 gtgacctggg tggtcccgct ggccgtgttg agcgagcgga cggaccaggc attgaggatg
     7501 tcgaggttca tggcggcgag agagacgggc gtggccgacg tggggttggc gagcgtcgcc
     7561 tccgcgcccg tcgcaacggc gaccgcagtc ggggcagcgg cgccggcgct gaccgcctgc
     7621 gacccgagga ggtccgcggg catcgtcacg ttcacgctgc tgtagtagta gaggatgacg
     7681 atgccgttca cgttcacaga gacggccgtg gtgccctggg cctgtgcctg ggccggctgg
     7741 accgcgccga acagggccgc ggcggcgacg aggacgaggg cgaggagctt catcgagttc
     7801 ttcatttcgt ttccttcctc caagttgggt ctctcactcc cggccgcggg gcccgccagg
     7861 ggcaccggag ccgcaaatac gttcagctga tccgcggggc gcctcggccg cccggattcg
     7921 aaccttctgc gatcgaccgc accgccccat ctccctcgga aaatcccggc gttgccgctc
     7981 gcacccgctc gaccattttc tgtgcgctcg agctagccat catggactcg cgagcgattt
     8041 ctgtgccaga ggtggcacac cgagagacac ctgagacacg caacgcgttt ctgtcgggaa
     8101 cggagcgaga agaccgcctc tctgcatcga ttccgcgtcg ttgaccggga aaaccccggg
     8161 cgatgtcgcg acagagtttc acaaactgat cccgcgcaag actctgattc gggacggcgt
     8221 tcccaaatag atgctcgggc gtctcacgac gctacaagcc cggggtcatt tcgcgtcagc
     8281 ctgcatggtc cgggaagtgc atggcattct gtaccgggag gagtgtccat ggctccagcc
     8341 gcagatcgca actggctcct cgccgcgctc ggctgtctcg cgctgtccgc ctccctggca
     8401 cctccggcag tgtcgcaggc catcgcgaac gggtcgttcg agacaggcaa cctgagcggc
     8461 tggacggtgg gcggcgtcgg gagggcggac gccgtgaggg cggacgactt cgaccggccc
     8521 ttttacgact tcgaccttcc gtttcccgac ggcacctggg cggcgctcct ctcgacgaac
     8581 tccacggcca acgcctcctc gaccgacctg gacggcaacg gagtccccga ataccacgtc
     8641 acgaccctgt cgcagacttt ctcggtctcg gcggctccgg tcaatctctc gctctcgtgg
     8701 ctcgtcctga cggacgaacg gaggcgcacc ggaggcggcg cggcggccta cgacgacctc
     8761 ttctcgatag acctcagcgg cacgcccatt ctccggcgga gcgtgtatcg gcccggtggc
     8821 gcgtcgccct ggaccgacac cccggcctac aacggaatcc gctacagggt ttcgaaaggc
     8881 ggaagcccgg tcaacggaag cgatttccag gggaccaacg gcggcggccg catgaccgcg
     8941 ttctccgacc tctgcgtcac catctcccag ccggggacct acaccctcac cttcaccgtc
     9001 gccgaccagg gggggaccga ctcgagctac gactcggcgc tcatggtcga caaggtcgag
     9061 ctcccctctt cctgcgtcgc ctcgccgatc cggatgaccg acacctccgg ggcgaacgtc
     9121 gaagtaaagt cgggcggcgt cgtcttcaac ccgcacttca accgccagcc ggcgacctcc
     9181 gacgacggct cggtcatcgc cttcgtctcg aacgcgaacc tcaccagcga caacccgaac
     9241 gccgtcgagc aggtcttcac gttcgcctcg aacgtctatc gccgcctgac gagcttcgcg
     9301 agcggaacgg tcggccgacc ctcgatcacg cggaacggcc gcttcgtcgc cttcgcgagc
     9361 aacgcgcccg ccgccgggaa ccccgacggg aactcggaga tctaccgcta cgacaccgtc
     9421 acctcgaccc tcacgcaggt gacgaatacg accggctgca cgaacggtgc gccctcgatc
     9481 tccgacgacc cggcggggag ctcgatcttc ttcgagacga cgtgcaacct cggcggcgcc
     9541 aactccaacg ggaatcgcga gatcgcccgc tggaccgggg actccacgtt cctcgtgacc
     9601 gccggctctc ccaccgcctg cacttctcac tcccctcacg ccgcatgggg cacgccgacc
     9661 acggcggtct tcgtctcgac ctgcggcttc accggggcgg ccaacgccga cgggaacgcc
     9721 gagatcttcc gctgggtcgc cccgtcctcg cccgggacct acacccgcat caccaacacg
     9781 acgggcgcca tcgccagcga cgccccgtcc atcaccgccg acggcgcgtt cgtctccttc
     9841 gtctccaacg cgaacctcgc ctcgtccaac gccgacggct cgttcgaggt cttccgctgg
     9901 cagtcgggca actcgtcctt cgttcaggcc accaacgacg cgagcggcct cgtcgcctac
     9961 acctcggccc gcatcggcgg ctcgaacggc aacctcatcg ccaccgagcg cctcagcctc
    10021 ctctccggga cgttcgagac gtcgctcctc acggtgggag ggggagaggc ggtcctcctg
    10081 acctccagcg acttcacgct ccccgccgtc gccgccgggg cgagcccccc cgcggtcgtc
    10141 ttccagtcct cggcggacct ccggggagag aaccccgatc ggaacctcga gatctgggcg
    10201 agcgattcgt cggtggggcg cgtccgcacc tacacccgca cgctcccccc gctcgccatc
    10261 ctccagtgcg gcggctactc gcagatctgc gagtgcacga acccgcgaac gaccactgac
    10321 gacctcgtcg tctccgaccc cggcacggtc ggctccctcg tcgtcagcct cgtcgtcacc
    10381 cacacctacg tcggcgacct gatcttccgg ctgacgcacg tcgagtccgg caccaccgtc
    10441 acgctcctcc accggcccct cgtccccgac ccgaacccca cggcccagtg cggctgctcg
    10501 aacgacaacc tcaacgtcgt cttcgacgac gccgccgcga ccgcggccga cggccagtgc
    10561 tccggcaatc cggcgatcaa cgggaggttc cgcccctata cgccgctctc ggccttcgcg
    10621 ggagcgcccc tcgccgggac ctggcgcctg acggcggtcg acatggcggg tcaggacacc
    10681 ggcagcatct cctcctgggg catgacggtc acgttgcagt agaggacgcg acgatgagaa
    10741 cgacgctccg ccacgccttc ctcctcttcg cgcccgcggt gatcgccctc ttcgccccga
    10801 ccgcgcccgg acaggcgctc ccggagccca tcgccccgcc cgacacctgg ggctacgaga
    10861 tgtacgacca gacgacgggg tcctgcccat tccagttcgt cgacctggcc agcggcggct
    10921 cgctcctcac cttccccgcg ccgggagatg acgagggggc cgtcctcgtc ccaacgcgcc
    10981 cgttccagct ctacggctcc cccgtcttcg gcttcgtcgc ctccacgaac gggtatctcg
    11041 ccgccaccgg ctccctctcc gcggaggacg ggcgcgacta cagcaacgac tcggtgccgt
    11101 ccgtccccgg ctacgtcccg ctcgtcccgg gcacgccgac cgcctcgttc cccggccgca
    11161 tcgccgtgtt tcacgacgac ctcgctctcg gcggcggctc ggtgcggtgg caggaggccg
    11221 cctcctgccc gcgcgcctcc ggcgcggtcg cgggggaagc ctgcacggtc gtccagtgga
    11281 gcggcgtctc ccgggctggc ggcagcgagc ccctcacctt ccaggcgatc ctctaccact
    11341 ccagcttcgc gatcgcgctc caggtcgcgg cgtccgacgg ctcgggcggt gggagcgcca
    11401 gcgccggcat ccaggacctc gaggcccgcc tcggcctttc ctacgccgcc aacgagccgg
    11461 gccgcctcgg cccgggcacc gccgtctgct tcttcgaccc gcggcatccg gccggcggcc
    11521 cgagagccga cctccggctc tccacgacgg ccaaggtcga gccgtccgcg cccgacgccc
    11581 tcgtcaccta cggggtcgcc gtcttcaacg ccggtccctc cgacgccacc ggcggcaccg
    11641 tcgacgcgcc gttcccttcc ggcctcgagt gggagtccga ctcctgcggc ggcgccttcg
    11701 ccgcaggcca gtgggtcctc gacgccctcc ccgccggagg ctccgccgag tgccacgtca
    11761 cggcccgcgt cgtcgcgacc gtcgaaggga tcgtcaccca ccccttcgtc gtgaacggcg
    11821 acgcgaccga tcccgatccg acgaacgacg gggtcgacgt cctcgtgcag gtcttcgtgc
    11881 ctgcgcccgt cgacgacgga gacggcgttc ccgcctcgac cgaagactcc ttccccggcg
    11941 ggctcggcgt tccgcccctc gcccccgggg acggcaacgg cgacggcatc gccgactcgc
    12001 tccaggccgg agtcgcttcg ctcccggcag cctcgggcgc aggctggctc accgtcgagc
    12061 tcctcggcgg cggctgttcg tccctgcagg aggtcgccgc gctggccgag agcgccgtcg
    12121 cggaaggcga cctgcagtac gacttcccgc acggcctcgt cgcgttccgg atcccgtgtc
    12181 cggcaggcgg cgcgagcgtg cgggtctggt atcacggagc ctctgccgtg ccgcccgtct
    12241 accggaagta cggacctctc gtcccgggag acggcgtcgc cgtctggtac acgcttcccg
    12301 gcgtcggttt cggtgagagc cgcggcatcc ccacggccag cttcactctc gtcgacgggc
    12361 agaccggcga cgacacgggg atcgacggcg tcatcgtcga ccagggcggc cccggccagc
    12421 cgggggagag cgtcgccacg attccggtgg ccggcccctg gggcctcgcg acgctcgcgg
    12481 ccctgatcct cgccgcgggg atcgcgctcc agcggtcgag gctcaggtag ccgtgccacg
    12541 acggaggagg ctgacgcctt ccgaggccgc tcgcgccgtc cgggccgcct gcttctctgt
    12601 gcccggaccc tgtgtcaggc ctgttccagg agacaccatc cccgccccac gtcccgcaca
    12661 accactttcc atacaatgac ttagacagcc gcttcggtgg catgacgctc gctcttcgtc
    12721 tcgtgaagag cgatgacgaa gcacgccgcg gcccgcagct cccgagcgac gacgaagccg
    12781 agatcggcct gggcgttccc ggcaattccg ctcgccctgg cggtcgcgat cctcgcgggc
    12841 cagcccgctg tcgcggcgac gagctccgtc gaggtctcgg ccttcatccg ggccacggcg
    12901 tcctgctccg tcgacctcca gcccggaagc gtccgcgtga gcgcggacga cgtccggcgc
    12961 ggctacgtcg acctgcccgc gtcgagccgg gtccgtgtcc ggacgaactc ctcgaacggc
    13021 tacatgctcg tcctcgaggt cgagagcagc gagttcgacg ccgtggtcct cgaggagtcc
    13081 ggcgagcggg tcgagatccg gggcactggt ttcgtcgagc gcccgtaccg cggccgcgcg
    13141 gaagtcgtct ccgagctcgg ctacagactc ctcttgaacc ccgccaccca gcccggcgtc
    13201 tacgcgtggc ccgtttccct ctccgcgcag ccgcgctgag cgaagagccg agacgccgct
    13261 ccgggccgcc gccgtcgttc ccgggcgtcg agcgctccgc ggcgagggat tcggtccgcg
    13321 gccgcgcgcg tcagaagttg agcgtcagcg tcacggagtc cgcgtagctc ccgggggcga
    13381 cgttctgctc cggcgggatc cgaccgtaga tcgggacgac gatcgacgtg ttgttcggcg
    13441 gcgacaaggg gccgtaacgc gagctccctc cgctgccgtc tccccagatc gtcgtgcagg
    13501 ccgaatcgag gaagagattg taggggagcg tgctgcccgt cgacgacatg cttcgcgggt
    13561 tgaaccgccc ggaggagccg ctcgacaggt cgatcgtgac cgtccgctgt ccgacgccgc
    13621 ggcaggtgta ggtcaccgag ccctgcgcat ctcgcggcga tgtccggaag acgtcgtagg
    13681 tggagaagga gacgggcgtg acgttctgga tcgagcaccg gatgtccgcc cgcgcgggcg
    13741 ctccgacgat cgcggccccc gcggcgaaga aggcgaggag caggcgaggt ctcatcgcga
    13801 cggccattct agcgacacgc accgggacgc ggatcatcga ttcggctcgc agacgagagg
    13861 cggcgcctcg aagatgaact ccgtcgaatc cggcacctcg agcgtgaagg tgcagggtcg
    13921 gcccgccggg ctcgcccgcc ccacgtaacg gccgggcgcg aggtcctcca ggtagaactc
    13981 tcccccggtt cccgtcgtgt gctcgaccgg gccggccgcg gtctcgagcg tgaagaccgc
    14041 gaactcgacc ggcacgacgt cgccctcccg gcgcgcgagg atcgtccccg tgacgccctg
    14101 cacccgctga acgtcgaacg acaagacgga gccgctccgg aaggggggag agacgacctt
    14161 cgtcagcgcg ggaacggagt agtcgagagg aatgtctcgg ttctcgatcg tcacctggtt
    14221 ctccaggaac gaagcgagct ccggaaggaa gacgatgccc gtgtcgtccg tccgtccgac
    14281 gacctggccg ttctgcgaga cgcggacccc ctcgagcgtt ccgacacggg ccacgccgaa
    14341 gctgtcggtg atcggtcgag tcaggcgcag cgcgccgccg gcccacgcga gcccgccggc
    14401 ggccgagagc ctgtaggtct cgctcggtcc ggcgccggga gcatccgaga gatcgactcc
    14461 cgccgtgaag agaccgaagc ggcgcttgac ctggacattc gccgagccct gggtctcccc
    14521 gccgccctcg agcggcaccc tcgcggacag accgtagccg atcccctccc cgaggggcgc
    14581 gcctcgaagc ccttcgacgg cgaaggactc ccggccgttc tcgcttcggg cgccggcgga
    14641 gagtgtcgcc tgccctccga ggtaggcggt gagcccgacg aagagctcgc gacgggagct
    14701 ctccccgctc gtccagcgcc cgctcaccct gagatgagcg gacctccaga cccttcggaa
    14761 gaacgtggtg gagacgctcg actccgtcgg cccgtcgaat cgttcccggc gagagagatc
    14821 gaaggcgagg gaaccgagcc ggggcgaagt ggcgttcaat ccgacgccgg tctcgacccg
    14881 ggtgcgcgcc gaggagctcg cctcgtccac cgtggcgtag cccgagctga acgacctcac
    14941 gaagagccgt ccgcccaggg agcggccgcc gtaaacccac gagagggctc cggcccaccc
    15001 cgtttccgcg ccgcgacccg cgctgacgtt cgccgcggcg gtcaaggtgc cccagcgccc
    15061 gaggacggcg gcgacatccg gacctgcgtt gaagacgtcg ccattcgcct ccgcacgcgc
    15121 ccccagggtc aggccatccg tgacgcccca gcggtggaag ccgaggaacg tgagggaccc
    15181 gtagtcgaag ctcgcctggc cgaattcccg gcgcggcgcc ccgatcgaat agctgaagtc
    15241 cgagaggccg cgggaaagac cgacgtcgga gaagtagaag gggacgaggt ggcgctcctc
    15301 gcgtccggcg acgtcccgga tgacgacctc gagcgtccgc gctcccgtgt agccgacgat
    15361 gttgcgaagg gagaacgtcc cgggcggaaa cgcctgcgtg gcgatcttct gcccgtcgag
    15421 gaagacgtcg acctgggacg gcgtcagcgc cgagccggtg aactcgaagg ccgggcgggt
    15481 gacgaggtag gggtcgatcc gaaaggcccg cgagtagctc acgccgccga ggccgagcgc
    15541 gctcccgaga aggccgctcg tcccgacgaa gtcgccggcg acgaagcggg tcaggcgctc
    15601 ccggtcgtcg cgcgtgagcg aggtcgagag ccggagccac cgctgctgct cgtccgtccg
    15661 ctcccagcgc ccctccccgt ggagcagcca gtcgccgagc cgcacgccca gctcctggcc
    15721 gaaggagagg ttccggaggc gcagcccggc ctccccggtg tagtcgagcc ggtagttgaa
    15781 gaacgccgag cggtctctcg ttcgaatcgc gtcgagccgg cgcttcggga ggagatcgag
    15841 cgtgctcgac gggaagctcg cgggggtcgc gaccaggtgg agcgtgaggg tcgccatgtc
    15901 gaggcggaac gtgatgcccg cggcctcgcc gagcgcgacc cacggctccc cgtcgatggt
    15961 ccggaccggc cgatccgcgg gcacgaggcc gagcgccgtc aggtcctccg ccttcagcag
    16021 gatgcctccg tcgtcctcga ggacggcgaa aagctcgcct ttcgggatct cgttcaggac
    16081 gacccggacc acgatcgtct ccccggcccg tgcggccgcg gaccacgcga gcaggagggc
    16141 gagaatcagg cagccgggaa ggcgctcagc gcgcacacgc cccgtcgggg acgtcgagac
    16201 cggcctcctg tttcaggtcc gcgccgacgg ccgcgacgtc gatccggacc aggccgcggc
    16261 attcctcctc ggtcaggcgc gcctggaagg gtcgcgtggc cccggcgagg acgtaccacc
    16321 cgccgatctc gcggctcagc ggctccccgc tggcgcgggt accgcaaagg aggaccttgt
    16381 cgacccggaa cgaaaccgat cccgtgttcc gaaccgggac gtggaccgta ccgtcgcgaa
    16441 cgtccagagc cacgatctcg gcccgggtcg acttcacggg cggctgcacg aagactccca
    16501 cgccgaaccg gacgcgcacg ttcacccgcg ccccaccctc cgggctcggc tcgggcatct
    16561 cctcgacgta aagccggtag gtcttctcga cggacgccgg gagcgaccgg acgcctatac
    16621 gcacgacggc ctccgcgccc ggctcgacgc tcaggatctt cgggaagaag acgaggtcct
    16681 tcgagtcgga atagacgtct cgtccgtccc cgtcctgcga ccattccgag agggtgacct
    16741 gcgcccgcag aggccgatcg ccgtcattgc gcacgttcac ggaagacgtt cgcgtggtcc
    16801 ggtcgaactc gatgcggatg ggcgagaccg agaactcggc gccggacgcc ggacgggcca
    16861 acacgaacgc cgcagtcaaa gccagggacg ggagggcggt ccatccggcc ttcatggaga
    16921 ggaatgttag cgtgtgtctc tccgccctac ggcaggagcg tgagggtgac cgtgtcggag
    16981 aaggagcccg cgatcgaggc ggcgacgtcg gccggcgtga cggtccccgt cagggtcagc
    17041 gtcccggtca ctccctttcc gatggtgccc gtcgcggggg agagtccgag gctgtaggga
    17101 aggtactcgt tcgaatccgt ggcgtgcttc atccggggac ggttcggtcc gaactcgttc
    17161 aggccgtcgt ccgaggagaa catgaaggag gcgatcggcg cacttccctg gcagcggaag
    17221 tcgagcgtgg cgctcacgac ccggtcggac gaaagggccg cgtccaacgt cccgaacgga
    17281 agcgccgcgg cactcgtccg gaacttgcag ttgctcttcg agagcacggt ggcggagacg
    17341 gagacgagcc cgccgtccgc gaccgcgccg tggctcatcg ccccgcagac gaggacgacg
    17401 agaacacgca gcaggcgtcg gcgcaacatc acgatcggag ctccccttcg cgacgcagaa
    17461 tcccgccgga gtccatccgt agcaggagaa gggggacgcg agccccgccc gcgtcccctt
    17521 tcgcgaggtc tcttgctggc ctaagggttg atcgtgagcg tgaccgtgtc gctgtacgcg
    17581 cccgcgagcg cgttcgcgta ggacgcgccg aggacctgag cggcgatgtc catcgtgaac
    17641 gggctctggg gcccggttcc cgtgccggac gcggtgtagg tgaaggagta cgggatgaac
    17701 tcggccgcgt tggtgccgtg caccatgcgg aaggtcgttc cggtctcgtt cagaccgttg
    17761 tcgtccgaga tcgtgtaggc cgcgcccttc gtgcaccaga actggggctg cgtgacggtg
    17821 cccgccacgt ccgttgccgc cgacgggtcg agagcgccga aggagacggc tcccgtcttg
    17881 ggcgaaacga acttgcaggt ccccaccacg ttggcggtca ctgtcacggt ggcggagtcg
    17941 ttcgccatca ggttcccggc cggaacgagg acggcgatcg cggcgagggc ggtcagggcg
    18001 atcagggcgg tcttcttcat ctcttcactc ccccagtccc gtcccatttc gggacgagac
    18061 gtgtcggtac ggatcattcg agtttcgttt cgggccgtgt gtcaggtgtc tcagggcaca
    18121 cgctgacaca cacgcaatac ggatgccggc ctccgcgaaa ggaatcccgg agtcctcgcc
    18181 gcctcgcctc tcaggatttc cgatcgggcg gagcggggcg gggctctcgc cccgtgtccg
    18241 ccgacggtct ccgcccctct ccccctgcgt cctgctccgt ctccgccgcc tcgtcccgga
    18301 cctgctcgac gagcgtgtcg acgcgccggt cggcctggag gtgcttctgg agctcgagga
    18361 ggccggcgcc ggcgaggttc gggcgggcgc tcggcgggcc gtagatccgc gcctgccgct
    18421 tcccctcgag atagacgacg aggacgagga gcgggactcc gacgacgaga acgaggccga
    18481 aagtcgtcat cagcttgatt gtgaacctgg gggcggccgc ctccctagaa tcgaacgcat
    18541 gtccgagccg cctacggttc cctcccccgg cgtcgagcgc ccccggcgct ccgtcctcta
    18601 cgttcccggc tcgaacgcga aggccctcgc gaaggcgcgg acgcttccct gcgacgccgt
    18661 gatcctcgac ctggaggact cggtcgcccc ggaggcgaag gaacccgcgc ggcgcgccgt
    18721 cgtcgaggcc gtcgccgcgg gcggcttcgc cccgcgcgag ctcctcgtga gggtgaacgc
    18781 gcgcgggacg ccgtggcacc gggaggacct cgccgccctc gcccgcctgc ccgtggacgg
    18841 catcgtcctg ccgaaggtcg agacgctcga agacgtccgg gaggccgccg gcgtcctcgg
    18901 cgcgtccggc gcgacggcgg caccggccct ctggctgacg atcgagacac cgcgaggtgt
    18961 cctggccgcc gacctcctcg ccggcgcgca cccgtcggtc gccggcctcg tcgccgggac
    19021 ctccgacctg acgaaggagc tgcgcgcccg gcacgtcccg gggcgggagc cgctcctcca
    19081 cgccctctcg gcgatcgtcc tggcggcccg ggcgcactct ctcgcggcgc tcgacggcgt
    19141 ccacctcgcg ctcgaggacg aggaggggtt cgccgcggcc tgccggcaag ggcgcgacct
    19201 cggcttcgac ggcaagacgc tgatccaccc gcgaacgatc gaggccgcga accgcgcgtt
    19261 cgcgcccgac ccttcggagg tggaagcggc ccggcggatc ctcgcggcgt gggaggccgc
    19321 gagggcagag gaccgaggcg tcgtcgtcca cgacgggcac ctcgtcgagg agctccacgc
    19381 gcgggaggcc gagcgactcg tcgcgctcgc cggagcgatc gcctcgcgga cggccggcgg
    19441 ctgacgcccc ggcgggtcag cggaccgtcg cgcagggccc ggccgcacga atcgctgcca
    19501 cgcgatcgac ccacgcgtgc cagacggccg gcggcacggg accgtccccc ggcgggatgg
    19561 cgttggctcg ccaggacgcc acgtcggcgc gggccgccgc gaggaagacc cggcaggcct
    19621 ccagcttcgg agcgcgggtg tagtagccga cgccgccgac gagcgtgccg acgccgccgg
    19681 cgccgacgag aagggccttc gtcgctcctt ccgatccggc gacgctcccg gacgaggcgg
    19741 cgaggacgcc gagcgccgcg cctccgacga accatgccct ccagatccgc tgctccctct
    19801 cgatccgttt cgtcgccttg tcccggagga gcggaagctc gttgtcgagg aaggagtcga
    19861 cctcgagccg gagctcggcc gcggtgggcg gcggctgctc ccggcgcagc tttcggcccg
    19921 cctcgacgag ggcccggtcg gtcccgcctc ccgatgcgca ggacgtgagg acgaggatcg
    19981 cgaggaggac cggtgcggac ttgcggcagt tcatggcggc tcggtttcgg aggatacgct
    20041 tcggcccgcg cattccgggg tgcgtaagat gccgccccgt gagccgtgtc ctctccctcc
    20101 acgacgtcgt gaagcactac ggttcggtga aggccgtcga cggcgtctcc ttcgaggtga
    20161 acgccggccg gatcaccggc ctcctcggcc ggaacggagc cggcaagacg acgactctcc
    20221 ggatgatcac gggggtcctg cggcccgacc gcggccgggt ggagctgttc agcgcggagg
    20281 tcgaggcggc gcgggaccgg ctcggctatc tcccggagga gcgcggcctc taccggaaga
    20341 tgcgcgtcct cgaccacctg ctcttcctcg ccgagatcaa ggggcgctcc ccggccgcga
    20401 tgcgcccggc cgccgagcgg tggctcaccc gattcgagct ccaggagaag aggaacgcga
    20461 aggtggagga gctctccaag gggaaccagc aaaaggtcca gctcgcgggc gcgctcctct
    20521 tcgacccgga gctcgtcgtc ctcgacgagc cgggctcggg cctcgacccg gtcaacgtcg
    20581 ttctcgtgag gcgccttctc cgcgagctgg ccgccgaggg gaaggcgatc ctcctctcga
    20641 cccaccagat gggcgaggcc gagaagctct gcgacgagat cgtcctcgtc cacgcgggga
    20701 aggtcgtccg ctcgggcccg ctcgccgagg tgaaggcctc gggcgggcgt gacgccgtcc
    20761 acctcgagtt cgccggagac ggcgcgttcc tcgcggagct ccccgccgtc gcctcggcgc
    20821 gggtcgacac gaaccgcgcc gagctgcggc tcgcgcccgg gggagacgcc caggaggtgc
    20881 tcgccgccgc ggcggcgcgg ctccgggtcc tccgcttcga ggtcgtcgcc ccctctctcg
    20941 aggaggtctt cctcgaggcg gtcggcgaga ccgctcctcc ggaggtggcg gcgtgaggaa
    21001 gctctgggcc gtcctgaagc gcgagtaccg cgagaccgtc cgcaagccct cgttcctgat
    21061 catgaccgtc ctcgccccgt tcctcttcgg ggcgctgatg ctcgtccccg cgctcctcgc
    21121 gacgcgcggg atgggcgagc ggaaggtcgc ggtcctcgac ggaacggggc gcctgcgcgc
    21181 cgcggtcgag gggctctcgg gggaggcgcc cggcgagcgc tcggccttcg cccgaggccg
    21241 ggggacgaac gtcccgaccg gccggatcgt ccccgagtac gtcgacctct tcacggtcga
    21301 cccgaagaac gcggtgggtc cctacctcga gcgactctcc gtcgagcgca cgccgaagga
    21361 gcagcgcctc gacggggtcc tcctcgttcc ggccgacgcc ttcgagcggc ccctcaccca
    21421 cctcacgtac ttcagccgct ccgcggccga cctcctcgcc caggagcgcc tcgggcgcgc
    21481 cgtgaaccgc gccctccagc gggaacgcct cgccgcgcgc ggggtgtcgc cggcggagct
    21541 ggagctgatc ctcgcgccgc tgccggtcga gaccgtccag gtgacgaagt cggggcagga
    21601 gcgccggggc ggagagggga gcttcgtcct cgcgatgctc ttcctcgccc tcctcttcat
    21661 cccttcgctc gtctacgggc aggaggtgat gcggggcgtc atccaggaga agacggaccg
    21721 cgtcgtcgag ctcctcgtct cgtcgatgac gccgatggag ctcctctccg ggaagatcct
    21781 cgggatggcg gcgatcggcc tgacgcagat ggccgtctgg gtgacgatgg gcggctccct
    21841 cgccgttgcc ggactctccg aggcgcgctc ggccggcttc gacctctcga ccgtcctccg
    21901 tccctcgatc gcggcctggt tcgtcgtctt cttcctcctc tcctacctcg tgacggtcgg
    21961 cgtctacgcc gcgggcgggg cgatcgccgg ctcggagaag gaggcgcagc aggtcctcac
    22021 gccggtgatg ttcgtcttcc tcgtgccgtg gttcctgatg atgccgatcc tgacgaaccc
    22081 cgactcgacg ctctccgtcg tcctctcgct cgtcccgatc tacacgccga tgacgatgtt
    22141 cataaggctc ctcgtctccg agccgccggc ctggcaggtc gccctctcgc tcgtcctgtc
    22201 cgtcgcgacg atcgccggcc tgatgaaagc ctcggccaag atcttccgcg ccggtctcct
    22261 ggcgaccggc aagcggccga cgatccccga gatctggcgc tggctgaagg aagcgtgagc
    22321 tgaaggcggc acgggcgcgt cggaaactcc gacgcgcccg tcggaaaatc cggcattcct
    22381 ggagcgctcc cagggcctgc ggtctcagga aagtaacgta aaccgctcgt tttgtgtttg
    22441 ttgcccgcgc gacccgaggg gcgaggcccg ccggcacgcc tcctgcagct ccagggttgc
    22501 gtcgacttcc cagcgaggga aatcccgacg cgaggaggtt tcccatgcag cccaccgact
    22561 ccgagatcct ctggctcaac ctgacgaacg ccggactcgg cctcgccacc ctgggcggga
    22621 tcgtcgctct cggctgggcg gccgtccgcg agctccgcga gcgcgcgaag ggccgcgtcg
    22681 ccgttcacga cctcgacccg cacgccgcct tcatccccga gctcggcctg acgatggccg
    22741 acggcggcga gaaggtcgac gagaacgagg agaagaagta gccccggggc tgcgagaggc
    22801 gtagccgtgc gttgcccctt ccttcgcgag gcgcacgtcc agtcgtgcca ggcctccccg
    22861 atccgcaagc agatcgtgcg gacggggagc gcatcggacg agcgctgcgc gacggcagcc
    22921 tggcacgagt gccccgtcgt ccgcgagctg aacgaggagc acccgagcgc gagctcctgc
    22981 ccgttccgga cggaatcgct cgcccagtac tgctccgcgg cgccggtgac gaagctgatc
    23041 ccctggagcg accaggcctt ctcccgctgc gggagcgagg cccaccgcta ctgcgacctc
    23101 tacctggcgc tcgcgaggac ccccgctccg accgcggccc cgggcgaggc cccgccccgg
    23161 tccgacgacg ccgacggcat cccggtcccg cagtggctct ggttcgcgcc ggggcacacc
    23221 tggctctcgg tcgcgtccga cggcgtctgc cacatcgggg tcgacgcatt cctcgcgcgc
    23281 acgctgggcg ggatcgaccg ggtgcagttc gtcacgacga aggggctcga gaggccggcg
    23341 gccgtcctca ccgtccgcga cgtggacgtg accgtctcct tcccgaaccc ggtcttcatc
    23401 accgccgtca acggcgcgct ccgcgcggac cccgcccgcc tcgcccgtga tccctacggc
    23461 ctcggctggc tcttcgaggg gatcgtccgc gaccgctccg ccgtcccgaa cggcgccccg
    23521 cggatcaccg acggtctcct tcgcggcgag gccgcgacct cctggatgcg ccgcgagacg
    23581 ctgcgcctgc gcgggctcgc cgaggaggcc gccgagcgga tctcgggcgt gcgcctcgcc
    23641 gccgacggcg ggctccccga gccggccctc gtccggaccc tgccccacga ggaggcgctc
    23701 cggctttccc acctcctcct ctccaccgct tccgacgaag agaggtctac ttgaagctac
    23761 cggccctcgt ctcgatcaca gccggcctcg tccccgccct ggcggcgggc tggctcctct
    23821 tcccgcgcct gatctacaag accgaggcgc agcccctccc cttcaaccac aaggtccacg
    23881 ccgagcaggg gatgtcgtgc ggcgactgcc acgccgtcgc ggaggacggc cgcttcgccg
    23941 gcatgccgac gaccgagagc tgcgccgggt gtcacgccga gaagggcgag aacgcgggcg
    24001 tcaacgccct cgtcgagcgc tacgtcgagc cgggggtcga gatcccgtgg ctctcgaacg
    24061 cccgccagcc cgacaacgcg tacttcccgc atgccgcgca cgtgaccggc gagaaggtcg
    24121 cctgcgcgcg ctgccacggc ccgcacgggg agtcgaccgc cgtgcgcccc gtccaggtga
    24181 accgcctctc cggctacccg cgcgacatct ggggcccctc gatctcgcgc ctccggcgcg
    24241 aggagtggca gggaatgaag atgtccgact gcatcgcgtg ccatcgcgcg ggcgggcgcg
    24301 agagcgcctg catcgactgc cacaagtgag gaggacggca tgaagctcga agtctcccgc
    24361 cgcgacctcc tcctcgccgg aaccggcgcc gccgccggga tcgccctgtc gccggtcccg
    24421 tggaaggccc tcgacgacct ctccatctgg acgcagcgtc ccccgacgcc gacgccgcgc
    24481 ccggccgggc cgctcgcgtt ccgcttcacg tcgtgcacgc tctgccccgc cgcctgcggg
    24541 ctgaaggcgc gctcgttcgg gccgcagccc gtcggcttct ccggcgtccc cggacacccc
    24601 gcgagcgacg gcggcctctg cccgctcggg ctgaccgccc accacctcgc gaagc
//
LOCUS       DAOONL010000051        24525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_1924_length_24525_cov_21.633633, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000051 DAOONL010000000
VERSION     DAOONL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24525
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_24525_cov_21.633633"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLP50_09370"
     CDS             <1..868
                     /locus_tag="PLP50_09370"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HPA51801.1"
                     /translation="SASLAFGRVASIRSSAERVLDALGPSPADALARDAWDAPRLDGF
                     VHRWVDSRSLRPFLRAVGATLRAEGSLAALFAAGDDGGPDFVPALDRFFTTLRERSEA
                     SPRAAPRGLLFLLPSPGNGSACKRAHLFLRWVVRTDDLDLGLWCGERFSPARLLLPMD
                     THVHRISRYLGLTARPTADLGAAREATAALARIDPEDPVRFDWALSRLGILAACVRDP
                     RRSLCGDCAVRPVCRAASPDGRPPVPSRRTGADPACSSRRPGSSSSPSRQSPRSPSAS
                     PTATPSGRARRR"
     gene            760..1335
                     /locus_tag="PLP50_09375"
     CDS             760..1335
                     /locus_tag="PLP50_09375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA51802.1"
                     /translation="MQLSKTRIVVLAVAAVATLALGLAYRDTLRKGPAKVTEPPAPAA
                     GRPGASPVSLRDGGTRDLSAPSGKLLVVHFWATWCAPCEEELPGLVAWWSEAKADPRI
                     ELVAVSVDEEWPTVEAFLAKRNAAGLPVALDPAKRAAFAFGTEKFPETWFLSPRGEVL
                     FHQVGPLDWGAPESRAALRALAEQALGPAAT"
     gene            1384..1980
                     /locus_tag="PLP50_09380"
     CDS             1384..1980
                     /locus_tag="PLP50_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51803.1"
                     /translation="MRELRSAVNAAFWFALFLLGWTLLVPPPEGTGKGSRPRFIRMTI
                     EDRGPGNHVERVRINVPWFLFRGGLHAVSASQIEHEADLQFQDPVTIDLLREIWKELS
                     EKPEGTDVARVHDDDELTFRKEKGEIRLTIREDVGESEGPPREIVTIRFPARFMEAAV
                     SGDRDLDLRALFEEMRGAARGDVLEVTSDDAHVRVVID"
     gene            complement(2114..2653)
                     /locus_tag="PLP50_09385"
     CDS             complement(2114..2653)
                     /locus_tag="PLP50_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51804.1"
                     /translation="MSAFPTQRPTVDVPSLSVRWEGLVVVIDEQTINTVLHRITRRVP
                     EVSDILVEAEAGVLSLTIKVKKGVRVPLKSRVTSIRFRDGWLGFALEELKAFGFVPIP
                     PWVVKRIVDRQPPGFAYFYPDERVVVLNLTTVLPPELSLEVREIRCENGEIRVAFGPS
                     RLRLDRLVASLDQDPFESD"
     gene            complement(2686..3669)
                     /locus_tag="PLP50_09390"
     CDS             complement(2686..3669)
                     /locus_tag="PLP50_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPA51805.1"
                     /translation="MTLRTAVRLTRPFTLLPPLLGIVSGAVCAFGSVHNPDPEHRLTW
                     AVVGAIALGSLCASFLNAASNVLNQITDLEVDRVNKPDRPLVTGEAGLRETWVLTWLL
                     YALAMVPVWFVVVPPREGLAARLLAPLATHQTFFLFLGGLVFTLVYSLPSWGRTKRLG
                     MWANVTIAIPRGCLLKVAGWTMVAPANALEPWVIGFLFFLFLVGAASTKDFSDVPGDR
                     AGGCRTLPILHGNRKAVAIMSPFFVLPWLLLIPLGFLADPLAPEHRLLTGNPWLLALL
                     GVVLTAWGLYTVRLLRRNPDELSTTENHPSWTHMYLMMMAAQVGFALAYVL"
     gene            3814..4845
                     /locus_tag="PLP50_09395"
     CDS             3814..4845
                     /locus_tag="PLP50_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPA51806.1"
                     /translation="MACKYGSHRVLEPKGGLPQPAQKLDNDFSKIEDNEILIDVKHLN
                     IDSASFTQLEAEAGGDVEKIAAKILSIVGERGKMQNPVTGSGGMFIGTVNKVGSALAS
                     RDLKPGDKIASLVSLSMTPLKIKRIIKVYADIDRVEVEAQAVLFESGIYAKLPDDMDE
                     GLALAALDVAGAPAQAAKLIRPGQSVLIIGAGGKSGMLVAYEAMKRVGPTGWVVGNVR
                     RAASIDDLKALDLCHAHVVADASKPVEFLNAVVAANKGREYDVVFNCVNIQSTEMSSI
                     LPCRQEGIVYFFSMATHFGKAALGAEGVGKDVTMLVGNGYSKDHAEITLAELRESPKL
                     RKFFETRYV"
     gene            4918..5742
                     /locus_tag="PLP50_09400"
     CDS             4918..5742
                     /locus_tag="PLP50_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPA51807.1"
                     /translation="MSEKVIITCAVTGAETTRESQPALPITPEEIAASAFEAWEAGAA
                     VLHLHVRNDDGSPTQDVNVFRKAIELVRAKCDIVIECTTGGAVGMTPEERLQPVTLKP
                     EMASLDCGTVNFGDEYIVNTLPIMREFARSMREHGVRPTLECFDLGHVYASHILIKEG
                     LLDEPYHYGLVLNVPGSVRYEVDVLEMFVRKLPKGAHWTLMGIGGKANLDAIYGALAL
                     GGNIRVGFEDNIYYSKGRLAKSNAELVERAARIAKDCGRPLARPDDVREMLKLRKA"
     gene            5752..7020
                     /gene="ablA"
                     /locus_tag="PLP50_09405"
     CDS             5752..7020
                     /gene="ablA"
                     /locus_tag="PLP50_09405"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPA51808.1"
                     /translation="MLYPKVDFRSIPLFSGVSDAEWNDWTWQIRNGIRDVETLEKVVK
                     LTDRERDEIRQCLGKFTMEITPYYAALMDRDDPFCPVRMQSVPRIAEMHDDPSDMADP
                     LHEDVDSPVPGLTHRYPDRVLLLVTNICSMNCRHCTRRRLVGDTDVDMPEDNVSAAIE
                     YIRNTPTVRDVLLSGGDPFVLPDERLRSILTRLRAIPHVEIIRIGTRTPVVLPQRITD
                     DLVGMLKEFHPLWVNVHFNHPKEITAEAKAACERLANAGIPLGNQSVLLRGVNDDPIV
                     MKKLVHKLMTVRVRPYYIYQCDLSLGISHFRTSVAKGIEIIESLRGHTTGLAVPTFVV
                     DAPGGGGKIPVMPNYLISMAEGKAILRNYEGVITSYSWPRGRGPANPATKDPGDDAKY
                     RAKDGVATLLAGDRRNLEPTGLARHKRNGD"
     gene            7087..7944
                     /locus_tag="PLP50_09410"
     CDS             7087..7944
                     /locus_tag="PLP50_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51809.1"
                     /translation="MPGALADVAGGVTAVTGFEKGSGKTTLLGALRPLARRAGPVALF
                     GIGVDGSLKAGGDGRAAVVRVEEGDVVQTTDLFARASSARLEILDVLPSRTSLGRLLL
                     GRAVRAGEVTLVGDESLSALSRSIDLVRDEQWAASVLVDGAASRITQAAALDGVRFLY
                     TVRVDGGNLSRVAERVWALAALAMLPVVPEPSEGTFRVEGPLTEEGREALPRDLGALS
                     LEDLTKSFLPPRLLLRLLETVACSVRRGIALLGVVALRRDVTAEELRAAVGPVAAPFL
                     LPSPFEAAA"
     gene            7941..9548
                     /locus_tag="PLP50_09415"
     CDS             7941..9548
                     /locus_tag="PLP50_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51810.1"
                     /translation="MNGPLAEYSGFATLWKRFAPETPFGRAASAAAEVLTDRAALEER
                     WDLTDEALRLLDVLEGDRVRLDRVRHHLRRIPRFPESPREAFDEVELFQLKKLLANHR
                     RLLALLPEGTKARFGLAPLPESLHALLGTGRDGDETFHVADAYDPELASVRGEIRRAD
                     AEAAAFRAARDEALRARCGFSLGDRPFLLVPREALGERAGVSDLLDVEPWDATRLCVR
                     PRPSVAELRIGEEKAALLARERGIEARVLRALSAPLSEALPLFLEQAGAVERLDLALA
                     GARLAREAGLVRPVLHDGPVRIVGGRHLPTEDLCAELGTPYTPLDAAFDGGPTVVFGS
                     NMGGKTVVLKTLALLQLAAQSGLFVPAARFETRLFRRFHYVGEGRGREAERGLSGFGF
                     EVRQLAEAHADFGDETLALFDELARTTSSVEAEALLSALLEELAARPRTVALFSTHFR
                     GVKRLPGVSYLRMAGLDRSRLELGAEGDDLPARIRRIDLLMRYHLEPDNPGERRSDAI
                     AVARLLGLSPGLSRRAEELLHDHDVKD"
     gene            9562..11127
                     /locus_tag="PLP50_09420"
     CDS             9562..11127
                     /locus_tag="PLP50_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPA51811.1"
                     /translation="MESRLHLPPERITRGRELASRIVAPVQDFIDAHTTVTVERATLR
                     LAGADGADADGVPVPNLVVDQVRDRLEEGALRPWVNALVRTGDDVAALSRRVAGGFRL
                     DGIPLADPVSLEAKAAELVSAASMRVRGNRKRREELLARYAGKNHSPLLYVIVATGNI
                     HEDVKQARAAASQGADVVAVIRTTAQSLLDYVPYGATTEGFGGTYATQENFRIMRAAL
                     DEEVERHGRYVHLTNYASGLCMPEIAVMGALERLDMMLNDSMYGILFRDINMYRTFVD
                     QKFSRMVNAWAGVVINTGEDNYLTTSDAVEKAYTVLASQFLNERFALVAGLEPWQMGL
                     GHAFEMDPSLPDGFLLEVAQALMAREIFPEAPLKYMPPTKFMTGDVFRGVVQNALFNF
                     ASKLTGQGIHLLGMLTEAIHTPFMQDRYLALENAKYVMGSMAGLGDEITFRPDGVIVR
                     RANQVLEEAVAFLEKVEGTGLMDAIATGLFADVKRPRDGGKGLDGLRKKGEGYWNPFE
                     THLTRELGLGGGR"
     gene            11124..11870
                     /locus_tag="PLP50_09425"
     CDS             11124..11870
                     /locus_tag="PLP50_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPA51812.1"
                     /translation="MTTPVRPYGDTLDDGAVQLSFTLPVPFGPRGREAARLLVEKLGF
                     RHAEVVHSAPLAEGFSFYVAYGRTDVSVDLDAVRVEEATGERVYTMDEACAVIREKLG
                     RKLVVVGACTGFDAHTVGIDAIMNMKGYNHHYGLERYSEVEAHNLGAQVPNERLIDYA
                     VSVNADAILVSQIVTQKDVHVRNLTELIELLEAKGLRERFVVCAGGTRIGNKLAVELG
                     YDAGFGPGTYADHVATFVIDRLVERAAARA"
     gene            complement(11940..13808)
                     /locus_tag="PLP50_09430"
     CDS             complement(11940..13808)
                     /locus_tag="PLP50_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA51813.1"
                     /translation="MREIRPKGAADPFRGLEYLPEPDYARPLRGLPPEIADRVLRRLA
                     LLYGAEAAAEVHRHVDRLVRVHDAYATAEIRAAEAAFDSRGRFSEKDAVLITYGDLVV
                     SEGRSPLATLSDFAYVLFRGLVTTVHVLPFFPSSSDRGFSIISYEEVDPKLGSWEEIA
                     RLGRGFRLMFDGVFNHVSAKSRRFQKFLAGDPEYQRFFRVFSSREEIDDDHLKLVLRP
                     RTSDLLSEFATVEGSRWLWTTFSRDQIDLNFRHPRVLLEVLEILLYYVRRGADLVRLD
                     AVTYIWHELGTKCAHLRQTHEVVKLVRDVLDAAAPHVALVTETNVPHADNVTYFGDGT
                     DEAQMVYNFALPPLVLHAFVTGSAEALSVWAAQLAPPSETTAFFNFLDSHDGIGLMGA
                     RGILPEEEIGKLCERVKAVGGLVSMRTGEGGAPSPYELNVTWWSALNDAEADEPLERK
                     VDRFLASRAIALSLRGVPGIYLLSFFGARNDFEAVKRDGQARSINRSAMREEWLFDQF
                     SDPTSIPSRTASRFIDLLGRRAAEPAFHPAAGQRVLRLDPRLFALVRTPRGGGAAVVV
                     VVDVSGTDVETTLDLSGAGLAATELVDLVSGRSFPSDRGLVPLRLRPYQVLWLRQK"
     gene            13914..15134
                     /locus_tag="PLP50_09435"
     CDS             13914..15134
                     /locus_tag="PLP50_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPA51814.1"
                     /translation="MSDFAQYGPITTLTRLGNRPLVELEDALVRHTRRNKAALLIPSL
                     ISELERPALLKICDEIAQAPYLDTVLISLDRADEAGYRFALDYFKRLKRRTVVLWNDG
                     PGVKALLEKLEKADLALGERGKGRACWIGFGYLLAQGNVEYIALHDADVLDYDRAMLA
                     RLLYPIADPILNFDFCKGYYARFTDRLNGRVARLFVGPLLGSLRSLLGANPYIEFLSA
                     FRYPLAGEFALSSDLLRQIRVPSDWGLEVGTLSEVYRHRSARRVCQVDIADRYDHKHQ
                     ALSQEDPTKGLHRMAADIAKHLLRTLAAADTVLGDGTLKSLLASYQRRAEDAASSFYA
                     LAKMNGLTFPRHEEEMAIATFAGALKNGVDQFLADPLGAPLIPNWARVLSAVPEAGDL
                     LLEAVTSEGGILNG"
     gene            complement(15144..16649)
                     /locus_tag="PLP50_09440"
     CDS             complement(15144..16649)
                     /locus_tag="PLP50_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HPA51815.1"
                     /translation="MSMVSMRARAGRLLPSAFALLAAPAALASEAVSTAPEEPLPIPL
                     DAYSGEAGMTLWRVLVHRVEEDPVNLVATVVFVLAILHTFLAPRITAAAHRLQHRNEK
                     ETGGDGHSFAVEMLHFLGEVEAVFGIWAIPLLLSAAALRGWHSVEEYVAHVHFTEPMF
                     VVVIMAIASTRPVLAFAERAMGAVASLAGRTPLAWWATILTIGPLLGSFITEPAAMTI
                     CALLLSRHLYELGPSPRLAYGTLGLLFVNVSVGGTLTHFAAPPVLMVAGKYGWGLTFM
                     LRTFGWKAALAVFVSTALFAFAFRKEFADLAGRRKARLADPVERPSERGVPAWIVVVH
                     LAFLGWTVFTAQTPALFVAGFLYFLAFTQATAPHQNPVNLRPALLVGFFLAGLVLHGT
                     LQQWWIAPVLARLEELPLFAGATVLTAFNDNAAITYLASLVPGFTPELKYAVVAGAVA
                     GGGLTVIANAPNPAGQSILSRYFPDGVSPLGLLLGALVPTGVVVLAFLFLP"
     gene            complement(16717..17073)
                     /gene="mnhG"
                     /locus_tag="PLP50_09445"
     CDS             complement(16717..17073)
                     /gene="mnhG"
                     /locus_tag="PLP50_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPA51816.1"
                     /translation="MSLRDLLAAALMLAGVATALLAAFGVVRMPDVPTRMSATSKAST
                     LAKMCVFLALALAFDDLGVAARSLAAVAFVFLTAPLAAHAIGRAAFALGVPLFPGTKV
                     NEMPRRNDGEGCDPDP"
     gene            complement(17070..17339)
                     /locus_tag="PLP50_09450"
     CDS             complement(17070..17339)
                     /locus_tag="PLP50_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPA51817.1"
                     /translation="MTLLSQLALWVGLPALGLALLLTLFRIVKGPTLPDRIVGLDLLG
                     TLGISIAAVVALSARDAVYLDVAIVLALLAFLGTVAFAAYLERSR"
     gene            complement(17336..17800)
                     /locus_tag="PLP50_09455"
     CDS             complement(17336..17800)
                     /locus_tag="PLP50_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPA51818.1"
                     /translation="MGTFAFLVFAWVAVTGEVSVANLLEGAVLAGLLVLVLRVPLRRR
                     FRLEKVPKALGLLLYFLKELLLSNAAVARILLSPASSLSPGIVAVPLDLKSDAGITVL
                     ANLVTLTPGTLSLDVSPDRTTLYVHALHVDDPDAFRREVKEGFERRVKEVFE"
     gene            complement(17804..19315)
                     /locus_tag="PLP50_09460"
     CDS             complement(17804..19315)
                     /locus_tag="PLP50_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011456809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HPA51819.1"
                     /translation="MNTLLVLPLLVPLATAAATILLARSPRAVRTASFAGAAASLGAA
                     VALLGATRDGTILVTQAGAWEAPFGISLVADRFAAAMVLVAALLLAAVVVSSIRGLDG
                     ARERAGYHPLLHVLSMGVSGAFLTGDLFNLYVWFEVMLMSSFVLVSLGGERAQLQGAL
                     KYVVLNLVSSALFLAGAGLLYGSIGTLNMAGAAVALRELPAQGLPSVVASLFLLAFGI
                     KAAAFPLFFWLPASYHTPPMPVAALFAGLLTKVGVYALVRVLTLVFVRDTAFTGPLLL
                     AVAALTMVTGVLGAMAQGDVRKILSFHIVSQIGYMLMGLGLGTRLALAGTVFYVLHHI
                     VVKANLFLVAGAMRRAGGSFSLEKLGSLLARSPLLAAAFLVPAVSLAGLPPLSGFWAK
                     YLLVRAGLEAGNGTIVAVSLAVSLLTLFSMTKIWNEAFWKPAPAGAGAAPAPLSAAER
                     ASLLGPVLVLGAITVAIGLGAGVLYRFADAAAAQLLDPELYVRAVLGARGGGT"
     gene            complement(19312..19656)
                     /locus_tag="PLP50_09465"
     CDS             complement(19312..19656)
                     /locus_tag="PLP50_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HPA51820.1"
                     /translation="MTLLLALAIGVLYAAGTYLLLRRSAIRVVFGLALLGYASNLLVF
                     TAGRLSREGPPIVPEGAVAAAATSADPLPQALVLTAIVIGFGVQAFALVLLKRTCQEA
                     GTDDVDAMREEG"
     gene            complement(19653..20069)
                     /locus_tag="PLP50_09470"
     CDS             complement(19653..20069)
                     /locus_tag="PLP50_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HPA51821.1"
                     /translation="MRSLILAAATRVVFPLLLLFSVFLLFRGHNEPGGGFVGGLVAAT
                     AYALLALTGGVAAARRVLPARPEVLIGTGLLAALASGLVGLAAGRPFMTGLWGDVSLP
                     VVGKPGTPVLFDAGVYLTVLGVVLLILLTLQEEDEA"
     gene            complement(20066..22384)
                     /locus_tag="PLP50_09475"
     CDS             complement(20066..22384)
                     /locus_tag="PLP50_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative monovalent cation/H+ antiporter subunit
                     A"
                     /protein_id="HPA51822.1"
                     /translation="MIAAVLSTFAVAALAPLLERRLGRRAAPLLALVPAALFVSFARH
                     VPEVSDGGTRTDALAWIAELGVSLSFRLDGLSLLMALVVTGVGAVVLLYAGSYMEGHP
                     RRGNLLGYLLAFLAAMLGLVLSDNLLGLFVFWEATSLLSYVLIGFEREKAEVRASALQ
                     ALLVTGGGGLALLAGFVLLGQAGGSLEIGALAANAEAVKASPLYLPALLLVLLGAFTK
                     SAQVPFHFWLPDAMAAPAPVSTFLHSATMVKAGVFLLARLHPTLGGTPEWTGIVTTVG
                     AATMLTGALLAVGQRDLKRLLAYSTVSALGSLVMLVGVGTPAALAAAMVLLLAHALYK
                     GALFLVAGIVDHETGTRDVASLGGLFRALPVTAVAAAAAAFSMAGLPPLLGFVSKELL
                     YDAALRASPAPLLLVPVAVGSNMLLVAVALSVGWGPFTGRAGETPKAPHEAPAAMWAG
                     PLLLGLGSLAAGLFSGAISPFVSAAAAAALGHPYGVKLELWHGFNLVLAKSLVTLVVG
                     VALFAARGRIRSLAGRLAPIAAYGPGRLYRAGLAGLVRFAKGTASVLSSGSLRQDLRI
                     VLGATVVLAAPLLLTRAVLGTPDALRSVRPLEAVLAFLVVAGAVATVLAGSRLAAVVA
                     LGVTGFGVALLFLVFGAPDLAMTQFAVETLSVLLFVAVLRRLPRLQSRSAPRARAVDA
                     LLAGAVGAVMTGLVLAVHAEPLRSRLAPFFRESSLALAKGRNVVNVILVDFRGLDTLG
                     EITVLGVAALGVFSLVRLRLSSRAAAAKGDRP"
     gene            complement(22466..23164)
                     /locus_tag="PLP50_09480"
     CDS             complement(22466..23164)
                     /locus_tag="PLP50_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51823.1"
                     /translation="MGKNLGQQIALAFERLLDAFLSVVPAALVLLAAALVGVLTGFVL
                     RALLRWAIRLRRKSRPDESSSSRQILRAAGLKADPERVAGTVSFWTAVVVALAVGVNA
                     LEPGALRNVLSEAVGYLPRLLGAALLAVVGLGIGALVRRSVLLGAVNAGLPWARMGAR
                     AAYVLVVASFAAAALDHLGVGRSILVAAFSIAGGAIALTLALAFGLGARDLARGWLEK
                     KMRAEEEDSGIRHV"
     gene            complement(23176..24450)
                     /locus_tag="PLP50_09485"
     CDS             complement(23176..24450)
                     /locus_tag="PLP50_09485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51824.1"
                     /translation="MTAHPLPPLSPEVADAIRKIGRVDLLVGIPSFNNERTIGHVVRA
                     VVAGLARHFPGAKAVLVNSDGGSTDRTRELVERTEVGSLTEILVDAVPTGATTRIVTP
                     YHGIPGKGSAFRTIFRIAAELEAEACCVLDSDLRSITPEWIELLLEPVVSHGFDFVAP
                     LYARHRYDGTITNTIVYPLTRALYGKRIRQPIGGDFGFTGDLAARFLEFGDWDGPVAQ
                     YGIDIFMTTTALAEGRKVAQSFLGAKIHDPKDPGSDLSPMMVQVVSTLFSLMERHEPA
                     WKRVAGSEKVPVFGFPYTVGLEPVPVKLDRLLAQYRQAAHDLPPIWESFLSPASLAAI
                     RDAAHAPAVSLGDEAWVRIIAEAAAGFRRRALPPATLIKSLVPLYLGKVATFVAETEA
                     GTAEEAEARLEELALAYERGKDALEALWGKVP"
BASE COUNT         3856 a         8643 c         9020 g         3006 t
ORIGIN      
        1 ctccgcctcg ctcgccttcg gccgggtcgc ctcgatccgc agctccgccg agcgggtcct
       61 cgacgccctc ggtccatccc cggcagatgc cctcgcgcgg gatgcctggg atgcccctcg
      121 gctcgacgga ttcgtccaca ggtgggtcga ttcccgatcc cttcgcccct tcctccgggc
      181 cgtcggggcg actctgcgtg ccgagggctc gctcgccgcc ctcttcgccg ccggggacga
      241 cggcgggccg gacttcgtcc cggccctcga ccgcttcttc accaccctcc gggaacggag
      301 cgaggcctcc ccgcgcgccg ctccccgcgg cctcctcttc ctcctcccct cccccggcaa
      361 cgggagcgcc tgcaagaggg cgcacctgtt cctccggtgg gtcgtccgga cggacgacct
      421 cgacctcgga ctctggtgcg gggagcgctt ctcccccgcc cgtctcctcc tcccgatgga
      481 cacccacgtc caccggatct cccggtacct cggcctgacg gcccgtccga cggccgacct
      541 gggagccgcc cgcgaggcga ccgccgcgct cgcccggatc gaccccgaag acccggtccg
      601 cttcgactgg gccctctcgc gcctcggcat cctcgccgcg tgcgtccgcg acccgcgccg
      661 gtcgctctgc ggcgactgcg ccgtccgccc cgtctgccgg gccgcgagcc ccgacgggcg
      721 accccctgta ccatcccgcc gcacaggagc cgaccccgca tgcagctctc gaagacccgg
      781 atcgtcgtcc tcgccgtcgc ggcagtcgcc acgctcgccc tcggcctcgc ctaccgcgac
      841 accctccgga agggcccggc gaaggtgacc gagccgcccg ctccggccgc cgggcggccc
      901 ggagcgagcc ccgtctccct ccgggacggc ggcacgcgcg acctttccgc cccgtccggg
      961 aagctcctcg tcgtccactt ctgggccacc tggtgcgccc cctgcgagga ggagctcccc
     1021 ggcctcgtcg cctggtggag cgaggcgaag gccgacccgc ggatcgagct cgtcgccgtc
     1081 tcggtcgacg aggagtggcc caccgtcgag gcgttcctcg cgaagcgcaa cgccgccggc
     1141 ctcccggtcg ccctcgaccc ggcgaagcgg gccgccttcg ccttcgggac ggagaagttc
     1201 cccgagacct ggttcctctc ccccaggggc gaggtcctct tccaccaggt cggccccctc
     1261 gactggggcg ccccggagtc ccgcgccgct ctccgcgccc tcgcggagca ggcgctcggc
     1321 ccggccgcga cctgacggga accttttcgc ccctcgtccg ttactgagga cggagagacc
     1381 gatatgaggg agctcaggtc cgccgtcaac gccgcgttct ggttcgccct cttcctcctc
     1441 ggctggaccc ttctcgtgcc gccgccggag gggacgggca aggggagccg gccccgcttc
     1501 atccggatga cgatcgagga ccggggcccc gggaaccacg tcgagcgcgt tcggatcaac
     1561 gtcccgtggt tcctcttccg gggcggcctc cacgccgtct ccgccagcca gatcgagcac
     1621 gaggcggacc tccagttcca ggacccggtc acgatcgacc tcctccgcga aatctggaag
     1681 gagctctcgg agaagcccga ggggaccgac gtcgcccgcg tccacgacga cgacgagctc
     1741 acgttccgca aggagaaggg ggagatccgg ctgacgatcc gcgaggacgt cggcgagagc
     1801 gaaggccccc cgcgcgagat cgtcacgatc cgtttccccg cccggttcat ggaagccgcc
     1861 gtctccggcg accgcgatct cgacctccgc gccctcttcg aggagatgcg cggggccgcc
     1921 cgcggcgacg tcctcgaggt gacgagcgac gacgcccacg tgagggtcgt gatcgactga
     1981 gtgcgggcgg gtaccttccg ccacctcggg gaggctcctc gagcctcccc gattccttcc
     2041 cccggtgccc cgctcgaggg gaggcgccgc agggcgccgc ggggaggctc gcggcgagcc
     2101 tccccgatcc acatcagtcg ctctcgaacg ggtcctggtc gagcgaggcg acgaggcggt
     2161 cgagtcggag gcggctcggg ccgaaggcga cgcggatctc gccgttctcg cagcggatct
     2221 cgcgcacctc gagggagagc tccgggggga ggaccgtcgt caggttcagg acgacgacgc
     2281 gctcgtcggg gtagaagtag gcgaagccgg ggggctggcg gtcgacgatc ctcttcacga
     2341 cccacggcgg gatcgggacg aagccgaacg ccttcagctc ctcgagcgcg aagccgagcc
     2401 agccgtcgcg gaagcggatc gacgtgacgc ggctcttcag agggacgcgg acccccttct
     2461 tcaccttgat cgtcagggag aggacgcccg cttcggcctc gacgaggatg tcggagacct
     2521 cggggacccg ccgcgtgatc cggtggagga cggtgttgat cgtctgctcg tcgatgacga
     2581 cgacgagccc ctcccacctc acggagaggg acgggacgtc gaccgtcggc cgctgcgtcg
     2641 ggaaggcgct catgacgccg gaatcctacc cgatcgggct ggcgctcaga ggacgtaggc
     2701 gagcgcgaag ccgacctgcg ccgccatcat catcaggtac atgtgggtcc aggacgggtg
     2761 gttctccgtg gtggagagct cgtcggggtt ccgccggagg aggcggaccg tgtagagccc
     2821 ccacgccgtc aggacgacgc cgaggagggc gaggagccag gggttgccgg tcaggaggcg
     2881 atgctccggc gcgagcgggt cggccaggaa tccgagcggg atcagcagga gccacgggag
     2941 gacgaagaag ggcgacatga tcgccacggc cttccggttc ccgtggagga tcgggagcgt
     3001 ccggcagccc cccgcgcggt cgccggggac gtcggagaag tctttcgtgg aggcggcgcc
     3061 gaccaggaag aggaagaaga ggaagccgat gacccacggc tcgagggcgt tcgccggggc
     3121 gaccatcgtc cagcccgcca ccttcaggag gcacccgcgc gggatcgcga tcgtgacgtt
     3181 cgcccacatc ccgagccgct tcgtccgccc ccacgacggg agcgagtaga cgagggtgaa
     3241 gacgaggccg ccgaggaaga ggaagaaggt ctggtgcgtc gcgagcgggg cgaggaggcg
     3301 cgcggcgagc ccctcgcgcg gcgggacgac gacgaaccag accgggacca tcgcgagggc
     3361 gtagaggagc cacgtcagga cccacgtctc gcgcaggccc gcctcgccgg tgacgagagg
     3421 gcgatcgggc ttgttgaccc ggtccacctc gaggtcggtg atctggttca ggacgttcga
     3481 ggcggcgttc aggaacgagg cgcagaggga gccgagggcg atggcgccga cgaccgccca
     3541 ggtgagccgg tgctccgggt cggggttgtg gacgctcccg aaggcgcaga ccgccccgga
     3601 gacgatgccg aggaggggcg ggaggagcgt gaaggggcgg gtgaggcgga cggcggtgcg
     3661 gagggtcatg ttcgcgcgct tcgagggtac accgaaggcc tgatatcctc tccggtcggc
     3721 cccggaccgg gcaccggctc cgcgggacgg catcgcggtg gaacgccccc cgccgggcgc
     3781 aacctgaaac gcgatcgcag agaggaatca acgatggcct gcaagtatgg ctcccaccgc
     3841 gtcctcgaac cgaagggcgg gcttccccag ccggcccaga agctcgacaa cgacttctcg
     3901 aagatcgagg acaacgagat cctgatcgac gtgaagcacc tgaacatcga ctccgcgagc
     3961 ttcacccagc tcgaggcgga ggccgggggc gacgtcgaga agatcgccgc gaagatcctg
     4021 tcgatcgtcg gcgagcgcgg gaagatgcag aaccccgtca ccggctccgg cgggatgttc
     4081 atcggcaccg tgaacaaggt cggctccgcc ctcgcgagcc gcgacctgaa gccgggcgac
     4141 aagatcgcga gcctcgtctc cctcagcatg acgccgctga agatcaagcg gatcatcaag
     4201 gtctacgccg acatcgaccg ggtcgaggtc gaggcgcagg cggtcctctt cgaaagcggc
     4261 atctacgcga agctcccgga cgacatggac gagggtctcg ccctcgcagc cctcgacgtc
     4321 gccggcgcgc cggcccaggc cgcgaagctc atccgccccg gccagagcgt cctcatcatc
     4381 ggcgccggcg gcaagagcgg catgctcgtc gcctacgagg cgatgaagcg ggtcgggccg
     4441 accggctggg tcgtcgggaa cgtccggcgc gccgcctcga tcgacgacct gaaggcgctc
     4501 gacctctgcc acgctcacgt cgtcgccgac gcgtcgaagc ccgtcgagtt cctgaacgcc
     4561 gtcgtcgccg cgaacaaggg gcgcgagtac gacgtcgtct tcaactgcgt gaacatccag
     4621 agcacggaga tgagctcgat cctcccctgc cggcaggagg ggatcgtcta cttcttcagc
     4681 atggcgacgc acttcgggaa ggccgccctc ggcgccgagg gcgtcggcaa ggacgtcacg
     4741 atgctcgtcg gcaacggcta ctcgaaggac cacgccgaga tcaccctcgc cgagcttcgc
     4801 gagagcccga agctccgcaa gttcttcgaa acccgctacg tctgagggcc ctccgcccgc
     4861 gtcgcgcccg tccggggcgc ggcgcgggcg gacgccgcgg ccctcccgga ggaccccatg
     4921 agcgagaagg tcatcatcac ctgcgccgtc accggcgccg agacgacgcg cgagtcgcag
     4981 cccgccctgc cgatcacccc ggaggagatc gccgcgagcg ccttcgaggc gtgggaggcc
     5041 ggcgccgccg tcctccacct ccacgtccgc aacgacgacg ggtcgccgac gcaggacgtg
     5101 aacgtcttca ggaaggcgat cgagctcgtc cgcgccaagt gcgacatcgt catcgagtgc
     5161 acgacgggcg gcgcggtcgg gatgacgccc gaagagcgcc tccagccggt gacgctgaag
     5221 cccgagatgg cgagcctcga ctgcggcacc gtcaacttcg gcgacgagta catcgtgaac
     5281 acgctcccga tcatgcggga gttcgcgagg tcgatgcgag agcacggcgt ccgcccgacg
     5341 ctcgagtgct tcgacctcgg ccacgtctac gcgagccaca tcctgatcaa ggagggactc
     5401 ctcgacgagc cataccacta cggcctcgtc ctgaacgtcc ccggctcggt ccgctacgag
     5461 gtcgacgtcc tcgagatgtt cgtgcggaag ctcccgaagg gggcccactg gaccctgatg
     5521 gggatcggcg gaaaggcgaa cctcgacgcg atctacggcg ccctcgcgct cggcgggaac
     5581 atccgcgtcg gcttcgagga caacatctac tacagcaagg ggcgcctcgc gaagagcaac
     5641 gccgagctcg tcgaacgcgc cgcgcgcatc gcgaaggact gcgggcgccc gctcgcccgc
     5701 ccggacgacg tccgcgagat gctgaagctc cggaaggcct gaggcgagga gatgctctac
     5761 ccgaaagtcg acttccgctc catcccgctt ttctccggcg tctccgacgc ggagtggaac
     5821 gactggactt ggcagatccg gaacggcatc cgagacgtcg agacgctcga gaaggtcgtg
     5881 aagctgaccg accgcgagcg cgacgagatc cggcagtgcc tcgggaagtt cacgatggag
     5941 atcacaccgt actacgcggc gctgatggac cgggacgacc cgttctgccc cgtgcggatg
     6001 cagtcggtgc cgcgcatcgc cgagatgcac gacgacccgt ccgacatggc cgacccgctc
     6061 cacgaggacg tcgacagccc cgtgccgggc ctgacccatc gctatcccga ccgcgtcctc
     6121 ctcctcgtca cgaacatctg ctcgatgaac tgccggcact gcacgcggcg ccggctcgtc
     6181 ggcgacaccg acgtcgacat gccggaggac aacgtctccg cggcgatcga gtacatccgc
     6241 aacaccccga cggtgaggga cgtcctcctc tcgggcggcg acccgttcgt cctccccgac
     6301 gagcggcttc ggtcgatcct cacgcgcctc cgcgcgatcc cgcacgtcga gatcatccgg
     6361 atcgggacgc gcacgcccgt cgtcctgccg cagcggatca ccgacgacct cgtcgggatg
     6421 ctgaaggagt tccacccgct ctgggtgaac gtccacttca accacccgaa ggagatcacc
     6481 gccgaggcga aggcggcctg cgagaggctc gcgaacgccg gcatcccgct cgggaaccag
     6541 agcgtcctcc tccgcggggt caacgacgac ccgatcgtca tgaagaagct cgtccacaag
     6601 ctgatgacgg tgcgggtgcg gccctactac atctatcagt gcgacctctc gctcggcatc
     6661 agccacttcc ggacgtcggt cgcgaagggg atcgagatca tcgagtcgct ccgcggccac
     6721 acgaccggcc tcgccgtccc gacgttcgtc gtcgacgcgc ccggcggcgg cgggaagatc
     6781 cccgtgatgc cgaactacct catctcgatg gccgagggga aggcgatcct ccgcaactac
     6841 gagggggtca tcacgtccta ctcgtggcct cgcggccgcg ggccggcgaa cccggccacg
     6901 aaggaccccg gcgacgacgc gaagtaccgc gcgaaggacg gcgtcgcgac gctcctcgcc
     6961 ggcgacaggc gcaacctcga gccgacgggc ctcgcgcggc acaagaggaa cggcgactga
     7021 tcgggcccgt gcgcgccgcg cgagccgggg gcgggacgcc gcgatccgcg gcgtcccgtt
     7081 gaggccgtgc ccggcgcgct cgcggacgtc gccggagggg tcacggcggt gaccggcttc
     7141 gagaagggct cggggaagac gacgctgctc ggcgccttgc gccccctcgc gcggcgcgcc
     7201 ggtccggtcg cgctcttcgg aatcggcgtc gacggaagcc tgaaggcggg cggcgacggg
     7261 cgcgcggccg tcgtgcgggt cgaggagggg gacgtcgtcc agacgaccga cctcttcgcc
     7321 cgcgcctcgt ccgcccggct cgagatcctc gacgtcctcc cgtcgcgcac gtccctcggg
     7381 aggctcctcc tcggccgggc cgtccgcgcg ggcgaggtga cgctcgtcgg ggacgagagc
     7441 ctctcggcac tctcgcgctc gatcgacctc gtgcgggacg agcagtgggc cgcctcggtc
     7501 ctcgtcgacg gcgccgcgag ccggatcacg caggccgccg cgctcgacgg cgtccgcttc
     7561 ctctacaccg tccgcgtcga cggcggaaac ctctctcgcg tcgcagagcg ggtctgggcc
     7621 ctcgccgccc tcgcgatgct cccggtcgtc cccgagccgt cggaggggac gttccgcgtc
     7681 gaggggccac tgacggagga gggccgcgag gccctcccgc gcgacctcgg ggcgctctcc
     7741 ctggaagacc tgacgaagtc gttcctgccg ccgcgcctcc tcctccgcct cctcgagacg
     7801 gtggcatgca gcgtccggcg cgggatcgcg ctcctcggtg tcgtcgcgtt gcggcgcgac
     7861 gtgacggcgg aggagctccg cgcggcggtc ggtccggtcg cggcgccgtt cctcctcccg
     7921 agcccgttcg aggcagcggc atgaacgggc cgctcgccga gtactccggc ttcgcgacgc
     7981 tctggaagcg cttcgcgccg gagactccgt tcgggcgggc cgccagcgcg gcggcggaag
     8041 tcctcaccga ccgggccgcg ctcgaggagc gctgggacct caccgacgag gcgctccgtc
     8101 tcctcgacgt gctcgagggc gaccgcgtcc ggctggaccg ggtccgccat catctgcgcc
     8161 gcatcccccg cttccccgag tcgccgcgcg aggcgttcga cgaggtggag ctcttccagc
     8221 tgaagaagct cctcgcgaac caccgcaggc tcctcgcgct cctgccggaa gggacgaagg
     8281 cgcggttcgg cctcgcgccg ctccccgagt cgctccacgc cctcctcggc acgggacgcg
     8341 acggggacga gacgttccac gtggccgacg cgtacgaccc ggagctcgcc tccgtccgag
     8401 gagagatccg ccgcgccgac gcggaggcgg ccgcgttccg cgccgcgcgc gacgaggcgc
     8461 tccgcgcccg ctgcggattc tcgctcggcg accgcccgtt cctcctcgtc ccgcgcgagg
     8521 cgctcggcga gagagccggc gtctcggacc tcctcgacgt ggagccgtgg gacgcgacgc
     8581 ggctctgcgt gcggccgcgg ccgagcgtcg cggagctccg gatcggcgag gagaaggccg
     8641 ccctcctcgc ccgcgagcgt gggatcgagg cgcgcgtcct ccgcgcgctc tcggcgccgc
     8701 tctctgaggc gctcccgctc ttcctcgagc aggcgggcgc cgtcgagcgg ctcgacctcg
     8761 cgctcgcggg ggcgcgtctc gcgcgggagg ccggtctcgt gaggccggtc ctccacgacg
     8821 ggcccgtccg gatcgtcggg gggcgccacc ttccgaccga ggatctctgc gccgagctcg
     8881 ggacccccta cacgccgctc gacgccgcgt tcgacggcgg gccgacggtg gtcttcggct
     8941 cgaacatggg cgggaagacg gtcgtcctga agacgctcgc cctcctccag ctcgccgcgc
     9001 agtcgggcct cttcgtcccg gcggcgcgat tcgagacgcg cctcttccgc cggttccact
     9061 acgtcggcga ggggcgcggc cgcgaggcgg agcgcggtct ctccggcttc ggcttcgagg
     9121 tccgacagct cgccgaggcc cacgccgact tcggcgacga gacgctcgcc ctcttcgacg
     9181 agctcgcccg gacgacgagc tcggtcgagg ccgaggcgct cctctcggcg ctcctcgagg
     9241 agctcgcggc gcgcccgcgg acggtcgccc tcttctccac ccacttccgc ggcgtgaagc
     9301 gccttcccgg cgtgtcgtac ctgcggatgg cggggctcga ccggtcgcgg ctcgagctcg
     9361 gcgccgaggg ggacgacctt cccgcccgca tccgtcgcat cgacctcctc atgcgctacc
     9421 acctcgagcc cgacaacccg ggcgagcggc gttccgacgc catcgccgtc gcgcggctcc
     9481 tcggcctctc gccgggcctc tcccggcgcg ccgaggagct cctccacgat cacgacgtca
     9541 aggactgaac caggaggccc catggagagc cggcttcacc tgccgccgga acggatcacc
     9601 cgcggccgcg agctcgcctc gcggatcgtc gcgccggtcc aggatttcat cgacgcgcac
     9661 acgaccgtta ccgtcgagcg ggcgacgctc cgcctcgccg gcgccgacgg cgccgacgcc
     9721 gacggcgtcc ccgtcccgaa cctcgtcgtc gaccaggtcc gcgaccgact cgaggagggc
     9781 gcgctccggc cctgggtgaa cgcgctcgtt cggacgggcg acgacgtcgc cgcactgagc
     9841 cggcgcgtgg ccggagggtt ccgcctcgac ggcatcccgc tcgccgaccc ggtctcgctc
     9901 gaggcgaagg ccgccgagct cgtctcggcc gcctcgatgc gcgtccgagg gaaccggaaa
     9961 cggcgcgagg agctcctcgc gcggtacgcc gggaagaacc actcgcccct cctctacgtc
    10021 atcgtggcga ccgggaacat ccacgaggac gtgaagcagg cgcgcgccgc cgcgtcccag
    10081 ggagccgacg tcgtcgcggt catccggacg accgcgcagt cgctcctcga ctacgtgccg
    10141 tacggcgcga cgaccgaggg cttcggcggc acctacgcca cgcaggagaa cttccggatc
    10201 atgcgggccg cgctcgacga ggaggtcgag cggcacggtc gctatgtcca cctgacgaac
    10261 tacgcctccg gcctctgcat gccggagatc gccgtcatgg gggcgctcga gcgcctcgac
    10321 atgatgctga acgactcgat gtacgggatc ctcttccgcg acatcaacat gtaccggacg
    10381 ttcgtcgacc agaagttcag ccggatggtc aacgcctggg cgggcgtcgt catcaacacc
    10441 ggcgaggaca actacctgac gacgtcggac gcggtcgaga aggcgtacac cgtcctcgcc
    10501 agccagttcc tgaacgagcg ctttgccctc gtcgccggcc tcgagccgtg gcagatgggg
    10561 ctcgggcacg cgttcgagat ggacccgtcg ctcccggacg gcttcctcct cgaggtcgcg
    10621 caggcgctca tggcgcggga gatcttcccc gaggcgccgc tgaagtacat gccgccgacg
    10681 aagttcatga cgggcgacgt cttccggggc gtcgtccaga acgcgctctt caactttgcc
    10741 tcgaagctga ccggccaggg gatccacctc ctcgggatgc tgaccgaggc gatccacacg
    10801 ccgttcatgc aggaccgcta cctcgccctc gagaacgcga agtacgtcat ggggagcatg
    10861 gcgggcctgg gcgacgagat cacgttccgc cccgacggcg tcatcgtgag gagagcgaac
    10921 caggtcctcg aggaggccgt ggcgttcctc gagaaggtcg aagggaccgg gctcatggac
    10981 gcgatcgcga cggggctctt cgccgacgtg aagcgccccc gcgacggcgg caaggggctc
    11041 gacggcctcc ggaagaaggg cgaaggctac tggaacccgt tcgagacgca cctgacgcgc
    11101 gagctggggc tcggaggcgg gcgatgacga cacccgtgag accgtacggc gacaccctgg
    11161 acgacggcgc cgtccagctc tccttcacgc tccccgtccc gttcggcccg cgcgggcgcg
    11221 aggcggcccg gctcctcgtc gagaagctcg gcttccggca cgccgaggtc gtccactccg
    11281 ctccgctcgc cgagggcttc agcttctacg tcgcctacgg gcggaccgac gtctccgtcg
    11341 acctcgacgc cgtccgcgtc gaggaggcga cgggcgagcg ggtctacacg atggacgagg
    11401 cctgtgccgt catccgggag aagctcgggc gcaagctcgt cgtcgtcggc gcctgcaccg
    11461 gcttcgacgc ccacaccgtc gggatcgacg ccatcatgaa catgaagggc tacaaccacc
    11521 actacggcct cgagcgctac tccgaggtcg aggcgcacaa cctcggcgcc caggtgccga
    11581 acgagaggtt gatcgactac gccgtctcgg tgaacgccga cgcgatcctc gtcagccaga
    11641 tcgtcacgca gaaggacgtc cacgtgagga acctgacgga gctgatcgag ctgctggagg
    11701 cgaaggggct ccgggaacgg ttcgtcgtct gcgcgggcgg cacccggatc ggcaacaagc
    11761 tcgcggtcga gctcggctac gacgccggct tcggccccgg gacgtatgcc gaccacgtcg
    11821 cgacgttcgt gatcgaccgg ctcgtcgagc gggccgccgc gcgcgcctga tgcgttccgc
    11881 gactgtgcgg gcggccgggt ccggccgccc gcacagagtg tagagtgtag accagacccc
    11941 tacttctggc ggagccagag gacctggtag gggcggaggc ggagggggac gaggccgcgg
    12001 tcggacggga aggagcggcc cgagacgagg tcgacgagct ccgtcgcggc gaggcccgcg
    12061 ccggagaggt cgagagtggt ttcgacgtcg gtcccggaga cgtcgacgac cacgacgacc
    12121 gcggccccgc cgccgcgcgg cgtccggacg agggcgaaga gccgcgggtc gaggcggagg
    12181 acgcgctgcc ccgccgccgg gtggaaggcc ggctcggccg cgcggcggcc gaggagatcg
    12241 atgaagcggc tcgcggtgcg cgaggggatc gaggtggggt cggagaactg gtcgaagagc
    12301 cactcctccc tcatcgcgga gcggttgatc gagcgggcct ggccgtcgcg cttcaccgcc
    12361 tcgaagtcgt tccgcgcgcc gaagaagctg aggaggtaga tcccggggac gccccgcagc
    12421 gagagggcga tcgcgcggga ggcgaggaag cggtcgacct tccgctcgag cggctcgtcc
    12481 gcctccgcgt cgttcagcgc gctccaccag gtgacgttca gctcgtaggg gctcggcgcg
    12541 cccccctcgc ccgtccgcat cgagacgagg ccgccgacgg ccttcacgcg ctcgcagagc
    12601 tttccgatct cctcctccgg gaggatcccg cgcgcgccca tgaggccgat accgtcgtgc
    12661 gagtcgagga agttgaagaa cgccgtcgtc tcggacggcg gagcgagctg ggcggcccac
    12721 accgagaggg cctcggccga gccggtgacg aaggcgtgga gaacgagcgg cggcagcgcg
    12781 aagttgtaga ccatctgcgc ctcgtccgtc ccgtcgccga agtaggtgac gttgtccgcg
    12841 tgggggacgt tcgtctccgt gacgagggcg acgtgcggcg cggccgcgtc gaggacgtcc
    12901 ctcacgagct tgacgacctc gtgcgtctgg cggaggtggg cgcacttcgt cccgagctcg
    12961 tgccagatgt aggtgaccgc gtcgagccgg acgaggtccg cgccgcgcct cacgtagtag
    13021 aggaggatct cgagcacctc gaggaggacc ctcgggtggc ggaagttgag gtcgatctgg
    13081 tcgcgcgaga aggtcgtcca gagccagcgg gacccctcca ccgtcgcgaa ctcgctcagg
    13141 aggtcggagg tcctcggccg gaggacgagc ttcaggtggt cgtcgtcgat ctcctcgcgc
    13201 gaggagaaga cgcggaagaa gcgctggtac tccgggtccc ccgcgaggaa cttctggaag
    13261 cgccggctct tggcggagac gtggttgaag acgccgtcga acatgagccg gaagccgcgc
    13321 ccgaggcgcg cgatctcctc ccagctgccg agcttcgggt cgacctcctc gtacgagatg
    13381 atcgagaagc cgcggtcgga cgacgagggg aagaacggga ggacgtggac cgtcgtgacg
    13441 aggccgcgga agaggacgta cgcgaagtcg gagagcgtcg cgagcggcga gcgcccctcc
    13501 gagacgacga ggtcgccgta cgtgatgagg accgcgtcct tctcgctgaa tcgcccgcgc
    13561 gagtcgaagg ccgcctcggc cgcgcggatc tccgccgtcg cgtaggcgtc gtggaccctc
    13621 acgagacggt cgacgtgccg gtggacctct gcggcggcct ccgcgccgta gaggagcgcg
    13681 aggcggcgga ggacgcggtc ggcgatctcg ggaggaaggc cgcggagggg gcgcgcgtag
    13741 tcgggctcgg gaaggtactc gagaccgcgg aacgggtccg cggcgccctt cggacggatc
    13801 tcccgcatga ggaagaccgt agcacgtccg ggagacggac tcctccgcgc gcccacggga
    13861 cggacggtcg ttttcgcttc cctcggcgcg ccggaccggg gtagaaagcc cccatgagcg
    13921 acttcgcgca gtacgggccg atcacgaccc tcacccgtct cggcaaccgg ccgctcgtgg
    13981 agctggagga cgccctcgtc cgccacacgc gccggaacaa ggccgcactc ctgatcccct
    14041 ccctcatctc cgagctggag cgcccggcgc tcctgaagat ctgcgacgag atcgcgcagg
    14101 ccccgtacct cgataccgtc ctgatctccc tcgaccgggc cgacgaggcg ggctatcgct
    14161 tcgcgctcga ctacttcaag cggctgaagc ggcgcacggt cgtcctctgg aacgacggcc
    14221 ccggcgtgaa ggcgctgctc gagaagctcg agaaggccga cctcgccctc ggcgagcgcg
    14281 gaaaggggcg cgcctgctgg atcggcttcg ggtacctcct cgcgcagggg aacgtcgagt
    14341 acatcgcgct ccacgacgcc gacgtcctcg actacgaccg cgccatgctc gcccggctcc
    14401 tctacccgat cgccgacccg atcctgaact tcgacttctg caagggctac tacgcgcgct
    14461 tcaccgaccg gctgaacggc cgcgtggcgc gcctcttcgt cggcccgctc ctcggctcgc
    14521 tccggtcgct cctcggggcg aacccgtaca tcgagttcct gtcggccttc cgctacccgc
    14581 tcgccgggga gttcgccctc agctcggacc tcctccggca gatccgcgtt ccgtcggact
    14641 gggggctcga ggtggggacc ctctccgagg tctaccgtca ccggtcagcc cgccgcgtct
    14701 gccaggtcga catcgccgac cggtacgacc acaagcacca ggcgctctct caggaggacc
    14761 cgacgaaggg cctccaccgg atggcggccg acatcgccaa gcacctcctc cggaccctcg
    14821 ccgccgccga caccgtcctc ggagacggga cgctcaagtc gctcctcgcc tcctaccagc
    14881 gccgcgccga ggacgccgcc tcgagcttct acgcgctcgc gaagatgaac ggcctcacgt
    14941 tcccccggca cgaggaggag atggcgatcg cgacgttcgc cggggccctc aagaacggcg
    15001 tggaccagtt cctcgccgac ccgctcgggg cgcccctcat cccgaactgg gcgcgcgtcc
    15061 tttccgccgt acccgaggcg ggcgacctcc tcctcgaggc cgtcacgtcc gaggggggca
    15121 tcctgaacgg ctgaagggca ccctcagggc aggaatagaa acgccaggac gacgaccccc
    15181 gtcgggacga gcgctccgag gaggagcccg agcggcgaga cgccgtcggg gaagtagcgg
    15241 gagaggatcg actgacccgc cggattcggc gcgttcgcga tgaccgtcag gccgcctccc
    15301 gcgacggccc ccgcgacgac cgcgtacttc agctccggcg tgaagccggg gacgagcgag
    15361 gcgaggtagg tgatcgccgc gttgtcgttg aaggccgtca gcaccgtcgc cccggcgaag
    15421 agcgggagct cctcgagccg cgcgagaacc ggcgcgatcc accactgctg gagcgtcccg
    15481 tggaggacca gccccgcgag gaagaagccg acgaggagcg cgggacggag gttcaccggg
    15541 ttctggtgag gcgccgtggc ctgcgtgaag gcgaggaagt agaggaagcc ggcgacgaag
    15601 agggccggcg tctgcgcggt gaagacggtc cagccgagga acgcgaggtg gacgaccacg
    15661 atccaggccg ggaccccccg ctccgacggg cgctcgacgg ggtcggcgag ccgggctttc
    15721 cgccggcccg cgaggtcggc gaactccttg cggaacgcga aggcgaagag ggcggtcgag
    15781 acgaagacgg cgagcgcggc cttccagccg aacgtccgga gcatgaacgt caggccccag
    15841 ccgtacttgc cggcgaccat caggacgggg ggcgcggcga agtgggtgag cgtccctccg
    15901 acggagacgt tcacgaagag gaggccgagc gtcccgtacg cgagccgcgg cgaagggccg
    15961 agctcgtaga ggtggcgcga gaggaggaga gcgcagatcg tcatcgcggc gggctccgtg
    16021 atgaaggagc cgaggagcgg cccgatcgtc aggatcgtgg cccaccaggc gaggggcgtc
    16081 cggcccgcga gggaggcgac ggcgcccatg gcgcgctcgg cgaaggcgag gaccggccgg
    16141 gtcgacgcga tcgccatgat gacgacgacg aacatcggct cggtgaagtg gacgtgcgcg
    16201 acgtactcct ccaccgagtg ccagccgcgg agcgccgcgg ccgagaggag gagcgggatc
    16261 gcccagatgc cgaagaccgc ctcgacctcg ccgaggaagt ggagcatctc gacggcgaag
    16321 ctgtgtccgt ctccgcccgt ctccttctcg ttccggtgct ggagccggtg ggcggccgcc
    16381 gtgatccgcg gggcgaggaa ggtgtggagg atcgcgagga cgaagacgac ggtcgcgacg
    16441 aggttcaccg ggtcttcctc gacccggtgg acgaggaccc gccagagcgt catccccgcc
    16501 tcgcccgagt aggcgtcgag agggatcgga agcggctcct ccggagcggt cgaaaccgcc
    16561 tccgacgcga gggccgccgg cgcggcgagg agggcgaacg ccgagggcag gaggcgcccc
    16621 gcgcgggcgc gcatcgagac catgctcatc gtgaccggat tgtggcatcc ccggagcgcc
    16681 gtccgctccg ccgtccttcg gagccgacgg tccgcttcag ggatcggggt cgcagccttc
    16741 gccgtcgttc cgccgcggca tctcgttcac cttcgtcccg gggaagagcg ggacgccgag
    16801 cgcgaacgcc gcgcggccga tcgcgtgggc ggcgagcggt gccgtcagga agacgaacgc
    16861 cacggcggcg agggagcggg ccgccacgcc gaggtcgtcg aacgcgagag cgagcgcgag
    16921 gaagacgcac atcttcgcga gcgtcgaggc cttcgacgtc gccgacatcc gcgtcggcac
    16981 gtccggcatc ctcacgacac cgaacgcggc gaggagcgcc gtggcgacgc cggcgagcat
    17041 cagcgcggcg gcgaggaggt cgcgcaggct caccgcgacc gctccaggta ggcggcgaac
    17101 gcgaccgttc ccaggaacgc gaggagggcg aggacgatcg cgacgtcgag gtagaccgcg
    17161 tcccgggccg agagggcgac gaccgcggcg atgctgatcc cgagcgtgcc gaggagatcg
    17221 agcccgacga tccggtcggg aagcgtcggt cccttcacga tccggaagag cgtcaggagg
    17281 agcgcgaggc cgagcgcggg caggccgacc cagagcgcga gctgcgagag gagcgtcact
    17341 cgaagacctc cttcacgcgc cgctcgaacc cctccttcac ctcgcgccgg aaagcgtccg
    17401 ggtcgtcgac gtggagggcg tggacgtaga gcgtcgtccg gtcgggcgag acgtcgaggc
    17461 tgagcgtccc gggagtgagc gtgacgaggt tcgcgaggac ggtgatcccg gcgtcgctct
    17521 tcaggtcgag ggggacggcg acgatgccgg gagagaggga cgaggcgggc gacaggagga
    17581 tccgcgccac cgccgcgttc gagaggagga gctccttcag gaagtagagg aggaggccga
    17641 gcgccttcgg caccttctcg agccggaagc ggcgccggag agggacgcgg aggacgagga
    17701 cgagaaggcc agcgaggacc gccccctcga ggaggttcgc gaccgagacc tcgccggtga
    17761 cggcgaccca ggcgaagacg aggaacgcga acgtgcccac cggtcaagtc cccccgccgc
    17821 gcgcgccgag tacggcgcgg acgtagagct ccgggtcgag gagctgggcg gccgccgcgt
    17881 ccgcgaagcg gtagaggact cccgcgccga ggccgatcgc gaccgtgatc gcgccgagga
    17941 cgaggaccgg accgaggagc gaggcccgct ctgccgccga caggggcgcg ggcgccgcgc
    18001 cggcgccggc cggggccggc ttccagaacg cctcgttcca gatcttcgtc atcgagaaga
    18061 gcgtcagcag gctgacggcg agggagacgg cgacgatcgt cccgttcccc gcctcgaggc
    18121 cggcccgcac gaggaggtac ttcgcccaga accccgagag gggcgggagg cccgcgaggg
    18181 agaccgccgg gacgaggaaa gcggctgcga ggaggggcga gcgggcgagg agggagccga
    18241 gcttctcgag cgagaagctc cctccggcgc gccgcatcgc tcccgcgacg aggaagaggt
    18301 tcgccttcac gacgatgtgg tggaggacgt agaagaccgt ccccgcgagc gcgagccgcg
    18361 tcccgaggcc gagccccatc agcatgtagc cgatctggct gacgatgtgg aacgagagga
    18421 tcttccgcac gtcgccctgc gccatcgccc cgaggacgcc ggtgaccatc gtcagcgccg
    18481 cgacggcgag gaggagcggg ccggtgaagg ccgtgtcgcg gacgaagacc agcgtgagga
    18541 cgcggacgag ggcgtagacg ccgaccttcg tcaggagccc cgcgaagagc gccgccaccg
    18601 gcatcggcgg cgtgtggtac gaggccggga gccagaagaa gagggggaac gcggccgcct
    18661 tgatcccgaa cgcgaggagg aagagggagg ccacgacgct cgggagcccc tgcgccggca
    18721 gctcgcggag cgcgaccgcc gcgccggcca tgttcagggt cccgatcgag ccgtagagga
    18781 gccccgcgcc cgcgaggaag agcgccgagg agacgaggtt caggacgacg tacttcagcg
    18841 ccccctgcag ctgcgcccgc tcgcccccga gggagacgag gacgaaggac gacatcagca
    18901 tgacctcgaa ccagacgtag aggttgaaga ggtcgcccgt caggaacgct ccggagacgc
    18961 ccatcgagag gacgtggagg agggggtggt agcccgcccg ctcgcgcgcc ccgtcgaggc
    19021 cgcggatcga ggagacgacg accgccgcca ggaggagcgc ggccacgagg accatcgcgg
    19081 ccgcgaagcg gtcggcgacg agggagatcc cgaacggggc ctcccaggct ccggcctgcg
    19141 tgacgaggat cgtcccgtcc cgcgtcgcgc cgaggagcgc gaccgccgcg ccgagggacg
    19201 ccgccgcccc ggcgaagctc gccgtccgca cggcccgtgg cgagcgcgcg aggaggatcg
    19261 tcgccgccgc ggtcgcgagc gggacgagga gcgggaggac gaggagcgtg ttcacccctc
    19321 ctccctcatc gcgtcgacgt cgtcggtccc cgcctcctgg caggtccgct tcaggaggac
    19381 gagggcaaac gcctggacgc cgaacccgat gacgatcgcc gtcaggacga gcgcctgcgg
    19441 gagcgggtcg gccgaggtgg ccgccgcggc gacggccccc tccgggacga tcggcgggcc
    19501 ctcccgcgag aggcggcccg ccgtgaagac gaggaggttc gacgcgtacc cgaggagggc
    19561 gaggccgaag acgacgcgga tcgcgctccg ccgcaggagg aggtaggtcc ccgccgcgta
    19621 gaggacgccg atcgcgagcg cgaggaggag cgtcacgcct cgtcctcctc ctggagcgtg
    19681 aggaggatca ggaggacgac gccgaggacc gtcaggtaga cccccgcgtc gaagaggacc
    19741 ggcgtccccg gcttcccgac gaccggcagg gagacgtcgc cccagaggcc cgtcatgaac
    19801 ggccggcccg ccgcgaggcc aacgaggccg ctcgcgagcg cggcgaggag acccgtgccg
    19861 atcaggacct ccgggcgcgc cggcaggacg cgccgcgccg cggccacccc gcccgtgagc
    19921 gcgaggaggg cgtaggccgt cgcggcgacg aggccgccga cgaaacctcc gccgggctcg
    19981 ttgtgcccgc ggaagaggag gaagacggag aagaggagga ggagcgggaa gacgacccgc
    20041 gtcgcggcgg cgaggatgag cgacctcacg gccggtcccc cttcgcggcg gcggcacggg
    20101 acgagagccg gagcctcacg agcgagaaga cgccgagcgc ggcgacgccg aggacggtga
    20161 tctcgccgag ggtgtcgagg ccgcggaagt cgacgaggat gacgttgacg acgttccgcc
    20221 ccttcgcgag agcgaggctc gactcgcgga agaagggggc gaggcgcgag cggagcggct
    20281 cggcgtggac cgccaggacg agcccggtca tcaccgcgcc gaccgcgccg gcgaggaggg
    20341 cgtcgacggc gcgcgcgcgg ggggccgaac ggctctgcag ccgcgggagg cgccggagga
    20401 cggcgacgaa gaggaggacc gagagcgtct cgaccgcgaa ctgcgtcatc gcgaggtcgg
    20461 gggcgccgaa gacgaggaag aggagcgcga cgccgaagcc cgttacgccg agcgcgacga
    20521 cggcggcgag gcgcgagccc gcgaggacgg tggcgacggc tccggcgacg acgaggaagg
    20581 cgaggaccgc ctcgagcggc cggaccgacc ggagggcgtc gggcgtcccg aggacggccc
    20641 gcgtcaggag gaggggcgcg gcgaggacga ccgtcgcgcc gaggacgatc cgcaggtcct
    20701 gccggagcga gccggaggag aggaccgacg ccgtcccctt cgcgaacctc acgaggccgg
    20761 cgaggccggc gcggtagagg cgcccggggc cgtacgcggc gatcggggcg aggcgccccg
    20821 cgagggaacg gattcggccc cgcgccgcga agagggcgac gccgacgacg agcgtcacga
    20881 ggctcttggc gaggacgagg ttgaagccgt gccagagctc cagcttcacg ccgtacgggt
    20941 ggcccagcgc ggcggcggcc gccgccgaga cgaagggcga gatcgccccc gagaagaggc
    21001 cggcggcgag gcttccgagg ccgaggagga gcgggccggc ccacatcgcc gccggcgcct
    21061 cgtgcggggc cttcggcgtc tcccccgcgc gacctgtgaa gggcccccat ccgacggaga
    21121 gggcgacggc cacgaggagc atgttcgagc cgacggccac cgggacgagg aggagcggcg
    21181 ccggcgaggc gcggagcgcc gcgtcgtaga ggagctcctt gctgacgaaa ccgaggagcg
    21241 gcgggagccc ggccatcgag aaggccgcgg cggccgccgc gaccgccgtg accggcaggg
    21301 cgcggaagag gccgccgagg ctcgcgacgt cgcgcgtccc ggtctcgtgg tcgacgatcc
    21361 ccgcgacgag gaagagcgcc cccttgtaga gggcgtgcgc gaggaggagg accatcgccg
    21421 cggcgagcgc cgcgggcgtc ccgacgccga cgagcatcac gagcgagccg agggcgctga
    21481 cggtggagta ggcgaggagc cgcttgaggt ccctctgccc gacggcgagg agggcgccgg
    21541 tgagcatcgt cgccgcgccg acggtcgtga cgatcccggt ccactccggc gtcccgccga
    21601 gcgtcgggtg gaggcgcgcg aggaggaaga cgcccgcctt caccatcgtc gcggagtgga
    21661 ggaaggtgct gaccggcgcg ggcgcggcca tcgcgtccgg gagccagaag tggaacggga
    21721 cctgcgcgga cttcgtgaag gcgcccagga ggacgaggag gagcgcgggg aggtagaggg
    21781 ggctcgcctt caccgcctcc gcgttcgccg cgagcgcgcc gatctcgagg ctcccgcccg
    21841 cctggccgag gaggacgaag ccggcgagga gcgcgaggcc gccgcccccc gtgacgagga
    21901 gcgcctggag ggcggaggcg cgcacctcgg ccttctccct ctcgaagccg atgaggacgt
    21961 acgagaggag gctcgtcgcc tcccagaaga cgaagaggcc gaggaggttg tccgagagga
    22021 cgagcccgag catcgccgcg aggaaggcga ggaggtaccc gagcaggttg ccgcggcggg
    22081 ggtgcccctc catgtagctc ccggcgtaga ggaggacgac cgcgccgacg ccggtgacga
    22141 cgagcgccat gaggagcgag agaccgtcca gccggaacga gagggagacg cccagctccg
    22201 cgatccaggc gagcgcgtcc gtgcgcgtcc cgccgtcgga gacctccggg acgtggcggg
    22261 cgaacgagac gaagagggcc gccgggacga gcgccaggag cggcgcggcg cgccgcccga
    22321 gacgtctctc gaggaggggc gccagcgcgg cgacggcgaa ggtggagagg acggcggcga
    22381 tcacgcggtc ccctagaacg cgcggaaggc ccgcgctatt ccgcccccgc gggcccggcc
    22441 ggcgagcggc gtcgggccca tcgcctcaga cgtggcggat cccggagtcc tcctcctcgg
    22501 cccgcatctt cttctcgagc cagccgcggg ccaggtcgcg cgcgccgagg ccgaaggcga
    22561 gggcgagcgt cagggcgatc gcgcccccgg cgatcgagaa cgccgcgacg aggatcgacc
    22621 gcccgacgcc gaggtggtcg agagcggccg ccgcgaagga ggccacgacg aggacgtagg
    22681 cggcccgcgc ccccatccgc gcccacggga ggccggcgtt cacggccccg aggaggacgc
    22741 tccgcctcac gagggcgccg atgccgaggc cgacgacggc gagcagcgcg gccccgagga
    22801 ggcggggcag gtacccgacc gcctcggaga ggacgttccg gagcgccccc ggctcgagcg
    22861 cgttcacgcc gaccgcgagc gcgacgacga cggcggtcca gaacgagacc gtcccggcga
    22921 cccgctccgg atcggccttc agccccgcgg cccggaggat ctgccgcgaa gaggaggact
    22981 cgtcgggacg cgacttccga cggaggcgga tcgcccaccg gagcagggcg cgcaggacga
    23041 agccggtcag gacgccgacg agcgcggccg ccaggaggac gagcgcggcc gggacgaccg
    23101 agaggaacgc gtcgagaagg cgctcgaagg cgagggcgat ctgctgtccg aggttctttc
    23161 ccatcgtcgt ctccgtcagg ggaccttccc ccagagggcc tcgagcgcgt ccttgccgcg
    23221 ctcgtaggcg agggcgagct cctcgaggcg ggcctcggcc tcttcggccg tccccgcctc
    23281 cgtctcggcg acgaacgtcg ccactttccc gagatagagc ggcacgagcg acttgatcag
    23341 cgtcgcgggc gggagggcgc gcctccggaa ccccgccgcc gcctcggcga tgatcctcac
    23401 ccacgcctcg tcgccgaggg agacggccgg cgcgtgggcc gcgtcgcgga tcgcggcgag
    23461 cgaggcgggc gagaggaacg actcccagat cggcgggagg tcgtgcgccg cctgccggta
    23521 ctgcgcgagg agccggtcga gcttgaccgg gaccggctcc aggccaacgg tgtacgggaa
    23581 gccgaagacg ggcaccttct ccgatcccgc gacccgcttc caggccggct cgtgccgctc
    23641 catgagcgag aagagcgtcg agacgacctg gaccatcatc ggcgagaggt cggagccggg
    23701 gtccttcggg tcgtggatct tcgcgccgag gaacgactgc gcgaccttcc gcccctcggc
    23761 gagcgccgtc gtcgtcatga agatgtcgat gccgtactgc gcgaccggcc cgtcccagtc
    23821 gccgaactcg aggaagcgcg cggcgaggtc gccggtgaag ccgaagtccc cgccgatcgg
    23881 ctggcggatc cgcttgccgt agagggcgcg cgtgagcggg tagacgatcg tgttcgtgat
    23941 cgtcccgtcg tagcggtgcc gcgcgtagag cggggcgacg aagtcgaagc cgtgggagac
    24001 gaccggctcg aggaggagct cgatccactc cggcgtgatc gagcgaaggt cgctgtcgag
    24061 gacgcagcac gcctccgcct cgagctcggc cgcgatccgg aagatcgttc ggaacgcgct
    24121 ccccttcccc gggatgccgt ggtacggggt gacgatccgt gtcgtcgcgc ccgtcgggac
    24181 cgcgtcgacg aggatctcgg tgaggctccc cacctccgtc cgctcgacga gctcgcgggt
    24241 ccggtcggtc gagccgccgt cggagttgac gaggaccgcc ttcgcgccgg ggaagtggcg
    24301 cgccaggccc gcgacgacgg cgcggacgac gtggccgatc gtccgctcgt tgttgaacga
    24361 ggggatcccg acgaggaggt cgacgcggcc gatcttccgg atcgcgtcgg cgacctcggg
    24421 cgagaggggc gggagcgggt gcgccgtcat gcctcctcct cgaggagctc gtcgagggcg
    24481 gcggcccagc cgcggcccgt cgggaagggg gcgacgcgcg cgccc
//
LOCUS       DAOONL010000052        23335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2093_length_23335_cov_18.853522, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000052 DAOONL010000000
VERSION     DAOONL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23335
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2093_length_23335_cov_18.853522"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..3125)
                     /locus_tag="PLP50_09490"
     CDS             complement(153..3125)
                     /locus_tag="PLP50_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51825.1"
                     /translation="MNLRVPALLFATAVLTSTGALGQAPTLVKDIYPGSSATATSRAG
                     FDRILGVSNGRVFLNASVYDPSMGLWVTDGTAAGTRQLLDIPTGYGADVGGTFYFSVG
                     SPWGVSLWKSDGTAAGTTFVTPVGADPFSYGGPFRLTRAGGRLYFLFDDGIHGCEPWT
                     SDGTEAGTMLLEDVTPGPDGTFSIGPNTYVSLVEAGGLLFFASPPLSSPGIGLWRSDG
                     TPAGTFQVADLSAVSSPVNVDGTLFFGASDGTHGIELWKSDGTVAGTVLVRDIVPGGA
                     SSSPTNLVAHGSALYFRAGQDGPIWKSDGTEAGTALVHPQTGSGPLVSSGDRVFSASG
                     SQLWASDGTEGGTVLVQGFGVSISLSTAATIPGALLLWVDRGVDGLELWRSDGTPAGT
                     TLVKAIEAGNEDASPYQSFSIPGAGVFAIGGTADLLWRSDGTPSGTYSLLEPVFPPND
                     SFPAYLTDVNGTVLFSAFDAEHGRELWRSDGTPEGTVLVKDLEPGPGSSSPSEFFVTP
                     SLVYFTAQTSATGFEVYRTDGTGSGTFLVKDVQPEAEYSPLLGLLGDLLLFPADDGVH
                     GLELWRTDGTADGTFLLGDLAPGAASSNPGAVGVLNGHFYFDASDGSTRALWRTDGTT
                     AGTVAVAAGLGSSGPAAELGGALYFSATDSTHGTELWRTDGTAAGTGLFRDINPTGDS
                     SVYVAGRLGDRLVFWADDGTHGFEPWTADGTSAGTSILKDLCPGPARSGPGALTFLGS
                     TLFFFAHDGIHGLEPWKTDGTTAGTTLVRDVAPGPVGSYFVGTFSTMGHEALFTASDH
                     VSGREYWRTDGTEVGTTQVADLVPGIGSGAPLWYTTGGRTPPVRSGGRMFFAANDGVT
                     GDELFSLPIPLGFHPVTPCRVADTRDPGGVAGGVPLGSAQTLILPVAGRCGIPSTAIS
                     VAANVTVVSPTATGSLSVFAGGPIVSGTTEVPVTVGKTRAMNAIPILGTTGSLSVRAG
                     LPEGGTTHVVLDVNGYFE"
     gene            complement(3181..6138)
                     /locus_tag="PLP50_09495"
     CDS             complement(3181..6138)
                     /locus_tag="PLP50_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51826.1"
                     /translation="MRSRLPLPLLATILLASEGALGQAPTLVKDIYPGTSSTASASAG
                     FERILGVSGGKGFLNGMEDGNQGLWISDGTAAGTHAILDLVPVAGVDVDGTFYFGAAA
                     QDGGSLWKTDGTAGGTVFVSRFSTDPSVIAHVSKPTKVGSRLFFAVDDGIHGAELWTS
                     DGTTAGTNLVKDLTPGSTGSLDYASELVDVGGTLFFTCRLDLGYGLWRSDGTEAGTTQ
                     LADLESVSSLVNVNGTLFFRATDPAHGSELWKSDGTPAGTVLVRDIVPGAAGSSPNAL
                     VAFGGALYFSLGSDGSTWKSDGTEVGTVLVHTFPTSDPLVVSGSHLYSVSGSQLWASD
                     GTSAGTVLVRDFGTAIFLWNSTTLAGTLHLWVDRGVDGLELWKSDGSPAGTTLVTVVE
                     PGNASPSPRGSVSIPGASLHLLSNMPFGLWRSDGTAAGTAPVQPPVFRPNDGVPVWLT
                     DVNGTLLFSATDAEHGAELWRSDGSPEGTVMVKDLAPGPAGSLPLQFQAASSIVYLQA
                     CPSSAGCQLYRTDGTEGGTALVKSIQPDSSVGHFRMLGLLGEQLLIAPNDGIHGNELW
                     RSDGTPDGTFLLGDLTPGPASSDLLALGTLGGSFLFSASSDATTTLWKTDGTAAGTTV
                     VGPMPVWNGGSAVLDDVVVFSASAAPHGTELWRTDGTAAGTTLLADINPSGDSSPSFA
                     GRLGDRVFFWADDGVHGTEPWVTDGTAAGTILLRDINPGPARSLGIRWTILGSTLYFF
                     AYDGIHGHELWKSDGTPAGTVLVRDVAPGPAGSMLLESFAAAGHEVFFTASDHVSGRE
                     LWRSDGTEAGTRLVADLASGLAAGTVSWTLSGNRVTLARSGGKVFFTASDGTSGHELW
                     ALPIPLGFHPVTPCRVADTRDPGGIAGGVPLANAQTLILPVAGRCGIPSTAISVAANV
                     TVVNPTATGSLSVFAGGPIVSGTTEVPVTVGKTRALNAIPILGTTGSLSVRAVLPEGG
                     TTHVILDVSGYFE"
     gene            6400..8682
                     /locus_tag="PLP50_09500"
     CDS             6400..8682
                     /locus_tag="PLP50_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51827.1"
                     /translation="MNDTDTAARPRRGGGRPLRLADGLAVVVGAFVLSLATIRAARVP
                     ITHDEALSFLRFVSAPLAEVLSFAGTDAANNHLLLTILERLLVSVLGPSELVLRLPVL
                     LSFAAGLAASWLLLRGRCAPALALAAFLTFATNRLGIELFSLARGYGLAVGLSVCGLL
                     ALVRSIERDFRPRPAVVGLTLLVLAALAQLTLLDVWAAASAAFLLVALRRYATGPGGA
                     ARSGWPRTLGVVAVPALLCAATAIPISFALSRGGALYAGGRMGFVDDTIGTVVRETLA
                     PAPWVDLLRGPATVALLVAAAFVLAAVGILLFSRAKRSGGPAALFVGATFLFYVLAIE
                     VQVRVFGVGYPVDRMALPLVPLLAFATALAAAVLLPGGSRLVRRGASALAVLLCVASV
                     AQLAATADVTRSRLWWFDADAPHVLDEIGRQTARLRPAEGSVSVGCSWILEPSLNYYR
                     VVRGLTWLLPFDRKGLAGERDFHVVATPDMASPEAAGLETVRTFAAAGTVLAVAGAER
                     LRSATPPLVATGGVLEPVVRAASGRREVTVWIPAVVHASGIGGALWRSDLRLRNRSDA
                     PARIVVGMSWKGERPQRALVVAGSGEALVPDVAGQLGVEGAGPLEVRIRGDVEVWARV
                     YDGATPNDEGVAAGSWFSAVHAAAGLGPGAVGRLEGLEESDRRRTNLGVLNLGPSPAE
                     VSVSWLGPRGNVLVSESRTLGPEEWRQETRPLAPHAEAAPICGATARVSVIRGTGIVA
                     WATVIDSVTHETRVVSARRE"
     gene            complement(8795..9496)
                     /locus_tag="PLP50_09505"
     CDS             complement(8795..9496)
                     /locus_tag="PLP50_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51828.1"
                     /translation="MMERRGIPPRATTSISVPLQLFPRLPHAPPPFPLLLGLHGYGMH
                     AATLFPLLARMAPEGWGVVAVEGPQSAFLPGEALGPSGARGFHWGVSPRHEDNQAVHR
                     AAVAEAIAWSVARGADPVRVSLVAFSQPCSFDYRLALAPPHGHPFRAVVALCGGLPGE
                     WTEPGPGTAASSATHALHVSTDADEYYPLERVAPFASLLASRFASARHEIHHGGHRIP
                     STAIPVVRAFLEAHG"
     gene            complement(9493..9888)
                     /locus_tag="PLP50_09510"
     CDS             complement(9493..9888)
                     /locus_tag="PLP50_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPA51829.1"
                     /translation="MLLSRAAETALKALPHLDPCGPGAPGRETRDLALACGEPAPWLG
                     KVLQKLARAGVLRSKRGRTGGFVLGRPAAEITLADVLLAIEGADDLERLLDVPAGPAG
                     PALLPARDALLSALRETTLESIVPPGDSR"
     gene            complement(9955..10344)
                     /locus_tag="PLP50_09515"
     CDS             complement(9955..10344)
                     /locus_tag="PLP50_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HPA51830.1"
                     /translation="MDWLLKTEPSTYSFEDLVREKAAVWDGVTNAQARLHLRSMKKGD
                     RLVVYHTGDVKAAVGRATVAAAPRPDPADPKGTVVDVKAGKPLKRPVTLAEMKAAKLF
                     AGSPLVTIGRLSVVPLTAEQYAFVVGE"
     gene            complement(10344..11672)
                     /locus_tag="PLP50_09520"
     CDS             complement(10344..11672)
                     /locus_tag="PLP50_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA51831.1"
                     /translation="MARLRALLARAVDLREGEGPALGLASAYFFLLLASYYVIRPLRD
                     EFGVRGSESALSWLFVGTLLGTAALNPLFSELVTRLTRKVFVPVVYRVLLGTLVLFWA
                     LLSFAPEGWRLPVAQAFFIWASVFNLFAVSVFWGFLADLFRPEQGKRLFGFIAVGGTL
                     GAVAGAALTASLAGPLGPTNLILLAAVLLEGAVFCVRGLVRHFHVDDSTRAAAPGAEP
                     EGVPPGSGALSGLATVSRSTYLLAISLFLLFFTVSSTFLYFEQARIVKATFTDSAART
                     AWFARIDLWVNLLSAFTQLFLAGRVVRLLGVGGTLVALPVLTLAGFAALAVSPTAGIL
                     ALVMVTRRAGNFALFRPAREVLFTVLPREEKYAAKSFVDTFVYRFGDVVGAFADKGLR
                     ALGAVGATLAGLFLPVAILWALLSAWLGRRQGKLAAARESGGTPADIIAA"
     gene            complement(11674..12807)
                     /locus_tag="PLP50_09525"
     CDS             complement(11674..12807)
                     /locus_tag="PLP50_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA51832.1"
                     /translation="MSRIARGTTRRDFLRTGAAAGAAFGLAGTALDALGAEPPARRKL
                     LILGGTAFLGPELVEAAQSARWTVTLFNRGKTNPHLFPDVEKLRGDRRSDLKALEGRS
                     WDAVVDTSGYFPKDVTASARLLAKSVGLYVFVSTISVYGDTSKPGIDERSPVAKTDQP
                     DADKVTGANYGALKALCEEAAERAMPGKALVIRPGLIIGPGDTSDRFTYWPVRVARGG
                     EVLAPGTPEDPVQFVDVRDLAAWTIAMAGKGRTGVFNATGPKEPLTFGPMLAACREAS
                     GSNATFTWVDAKTLEELKIAPWSDMPCWVPPFGETAGSRRVNNGKAVGEGLTFRPIVE
                     TARDTLAWWKTLPEERRSRLKAGLTAERETAALATVREKKAKG"
     gene            complement(12904..14055)
                     /locus_tag="PLP50_09530"
     CDS             complement(12904..14055)
                     /locus_tag="PLP50_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51833.1"
                     /translation="MTFAGYVLRHLRARPLRASLTAGAFAFSIGLLGFLLLLSSALRK
                     EWSPHMAQRMIVTAKSSFFEKLPMSYMAKIEATPGVDTVVPFDFLVGFYRDARPENQV
                     PIQSAPPEPMLRVYMEAEVPADQAKAWVDDPTGALVGPLLAEKLGWKVGDRVVLQAPV
                     PGGVVETTIRAVMGYRLDAGLYLHRRYFSQLTADEGRTNMFWVLAKSRGDVDRVTAAL
                     ARALENAPVPVLAMTERQWQLQFLEMLGNVNALLGSVGVATGFALLLITANSLAMSAR
                     ERRNESAVLRVLGFPRKAIAGLLLGEALVYGLAGALLGGGVMWLFARGIGAALDKTQY
                     AGMGQLLVPDAPALFFTALVSMLLALFAGLAPALNLARRPVVELLREAG"
     gene            14147..14551
                     /locus_tag="PLP50_09535"
     CDS             14147..14551
                     /locus_tag="PLP50_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HPA51834.1"
                     /translation="MRSLLVPLLAVPALLAAPPTADVSPLRITELSVCREIVDRGCES
                     EGRTFGPDVESVAVLTRVEGATGEAFVFHVWSFEAKEVQRVRLPVRAAVYRTWSKKSV
                     KGQPGRWKVEVLDPIGRSLGVVDFAVQPPKID"
     gene            14635..14709
                     /locus_tag="PLP50_09540"
     tRNA            14635..14709
                     /locus_tag="PLP50_09540"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14667..14669,aa:Val,seq:tac)
     gene            complement(14717..15202)
                     /locus_tag="PLP50_09545"
     CDS             complement(14717..15202)
                     /locus_tag="PLP50_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPA51835.1"
                     /translation="MVSETYLPPARLLLSSRWAALAVVVDGAPAAAMTSYALAPDGAS
                     LYLHLSQMALHTRALLTEPRAALAVSAPDTGDGDPQTLPRLSLSGVALALVPGTPEYE
                     TGQAAYVSRFPAAGERFALADFVLFRFEPSEARWVGGFARALRMSGTQLAEALAEAGR
                     G"
     gene            complement(15213..16145)
                     /locus_tag="PLP50_09550"
     CDS             complement(15213..16145)
                     /locus_tag="PLP50_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA51836.1"
                     /translation="MRIVVTGAAGFLGSHLADRLLAEGHEVVGIDNLITGHVRNIAHL
                     MGNRAFRFLHHDVTEYIYLDGPLDAVLHFASPASPIDYLQIPIQTLKVGALGTHKALG
                     LSHAKKARFLLASTSEVYGDPLVHPQPETYWGNVNPVGPRGCYDEAKRFAEAMTVAYR
                     NFHGVETRIVRIFNTYGPRMRANDGRIVPSLINQALAGEPLTVFGDGSQTRSFCFVSD
                     LVDGIYRLLLSDQSDPCNVGNPAEMTVMQFAETIRRLTGTKSTIVHKPLPVDDPKQRR
                     PDISLAKAKLGWEPKVSLEDGLAATIDYFRALRG"
     gene            16227..16736
                     /locus_tag="PLP50_09555"
     CDS             16227..16736
                     /locus_tag="PLP50_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51837.1"
                     /translation="MPGLGEDGMRVVTLRSLVGFLVLVFAVAGPLAADPPTLEGTWLF
                     DARASKNVPEALKGVDLKIDVKDPQVTITKLAGERPVGEPMVVMLDGKSRPQDVGGHR
                     ATVTAKWLEKGRRFEHVISMMQAGSVFATTQTIVTEVSETGGTMLRKYSIRQARETQE
                     RALVYRRKP"
     gene            complement(16807..18105)
                     /locus_tag="PLP50_09560"
     CDS             complement(16807..18105)
                     /locus_tag="PLP50_09560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPA51838.1"
                     /translation="MNICMVGTGYVGLVTGACLADFGMDVTCIDKDETKIALLKKGVS
                     PIYEPGLEELIHKNAQAKRLHFATEIREAVERALVIFIAVGTPPRADGSPDLSFIFQV
                     AEAISEHMNGYKVVVTKSTVPTGTGAQIEEILRKNPGGHKFSVVSNPEFLREGSAIED
                     FMRPDRVVIGSRDAEAIAIVKDVYSPLIVAGVPFVVSDVESAELIKYASNGFLALKIS
                     FINEIAAMCERMGADVKDVARGMGLDKRISPHFLLPGPGFGGSCFPKDASGVVDVARK
                     HGYTFEIMETVLRVNATVKARMVEKVEKVCGDLAGKRVAVLGLAFKPETDDIRDSSSL
                     KLIEDLVARGASVAAYDPAAMENTKAVLPGIEYAEDVYACAAGADALVLATDWNQFRK
                     LDLARLEATMKAKRFVDLRNLYEPREMKRLGWEYVAIGRA"
     gene            complement(18182..19225)
                     /locus_tag="PLP50_09565"
     CDS             complement(18182..19225)
                     /locus_tag="PLP50_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA51839.1"
                     /translation="MAFRVLVAGGAGLVGSAVARQLLEEGADVTVVDALVAEGDGRAV
                     REARLETLRAYPNAKVVTADLSLPGAAAEVFREARPGAVVNAALFPPGGPGLDAMLEA
                     ARAHEKPFLVHLSDAALYGPPGEAGQPATEAEPIDPGRDPVLLQRMVEESRLSASYLS
                     WVVLRLFGLLAPGAPPTRFPQDALESLLAGEEIVLPSDAPRDLLHVGDAVHGILAALA
                     LRPEGRTINLGSGRGVAPSFVLHLLGDRASRPVRYRVSGETFRPGRIADLALAGELLR
                     FRPPTPLETALTEIVTARLGSPQIPTSRPGPALRPVWPAPAAATAPDAPREVSRRELF
                     GFFRGPLGGGRSR"
     gene            complement(19228..20433)
                     /locus_tag="PLP50_09570"
     CDS             complement(19228..20433)
                     /locus_tag="PLP50_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPA51840.1"
                     /translation="MKADSRTRSRVGVIVFAAILLFAVMVVVVGGKTGFFLARSSYFA
                     RFPNSQGLSGGNQVRLAGVTVGAVQEVEVPKSPGEDLTVRFDIERRYRHLVRTDSRVE
                     IKTIGLLGDKYLEVTPGSPGTPLLDPGSEIAAIRGSELDKILAGSGDLFENVNSVAES
                     LKTILGRVEKGEGFLGEMTSASPEGKELSRSLRQTIAATNALLADVRAGKGLVGRLVA
                     DEKLGDEVAAELAASAASLRKLLAAVERGAGDGEGLVPALLGDPEGKKKFFAMVDSLN
                     ATAEGLSGFSRELSSGSGLLPKLVRDEAFAKELTEDLRRISSSLASVAEKVDRGEGTA
                     ARIVNDPALFEAIDDILVGVNESKLLRWLVRNRQRSGIQARYEETTAKGAAPAPEALG
                     PAPTPEPRE"
     gene            complement(20450..21199)
                     /locus_tag="PLP50_09575"
     CDS             complement(20450..21199)
                     /locus_tag="PLP50_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA51841.1"
                     /translation="MSEPVVELRAVSKAFGEKVVLDAVDLAVARGEVLVILGGSGSGK
                     SVTLRHMNGLTQPDAGSVFVEGVEVSRLAEEELGEVRRKVGMLFQMGALFDSMTVFEN
                     VAFALFEHTKMTGEEIAARVTEVLGFVDLGPDVLPLLPSSLSGGMKKRVSLARTIALK
                     PDVLLYDEPTTGLDPVTAMTINRLIVDLNSRLATTSVVVTHDIASALFVADRIAFLEK
                     GRFAFVGTPGEARSSDVAALRAFLAAEEAGA"
     gene            complement(21196..22014)
                     /locus_tag="PLP50_09580"
     CDS             complement(21196..22014)
                     /locus_tag="PLP50_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51842.1"
                     /translation="MSEASAPDGRPAPFWTLPRLRRWFRKVVSHLGGLGLLAWSIVRE
                     GRRRWEGGEILRQMDALGVQSISVANLTCLFTGMVLALQTAHSLAEFGGKLFVGRAVI
                     LSLARELGPVLTALMLAARCGAGITAELGTMAVTEQVDALRALGSSPIRKLVLPRVVA
                     LTVMLPVLTLIAIFHGMLGGLFIAVFELKIGASFYLSTTLQALSFNDLMHGLCKTPFF
                     GLEIALIGCYNGLSATGGADGVGRATTVAVVVASITILITDFFLSKLFIALPIG"
     gene            22055..22768
                     /locus_tag="PLP50_09585"
     CDS             22055..22768
                     /locus_tag="PLP50_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA51843.1"
                     /translation="MQLLYTTWVWLFGLLVTPVFGTLAILTSWIPPRGRVYQYWARWW
                     SRTFLAGAGIDVRVETSEAARTVPEAIFMANHTSAADIVVLFVALARDVRFVAKKQLF
                     WIPFLGWSMWLAGFIPVDRENKEKAREAFDQIGKQLRKGASILVFPEGTRSRNGKLGA
                     FKKAGFLLAMRTSLPIVPVGISGARAVLGADGLLVRRGRIEVRVGDPIPSDGYTFARR
                     AEYVERVRAEILRLGDSAG"
     gene            22849..>23335
                     /locus_tag="PLP50_09590"
     CDS             22849..>23335
                     /locus_tag="PLP50_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPA51844.1"
                     /translation="MSNLNEDAVLEALRKVKYPGFSRDIVSFGFVKDVAVGGGNVSFR
                     LSMTTASAEIAEQIRVEADAALRTLPGVSAVTIKVETGQAPVNLSGAQGARPDVLPET
                     RFTVAVASGKGGVGKSTVAANLALALRQLGYTVGLLDCDMYGPSQQMMMGIDEKPFLN
                     DE"
BASE COUNT         3449 a         7813 c         8797 g         3276 t
ORIGIN      
        1 tctcggggat ctcgagcctc gggtcggcct cggcctcgat ctcgaggagg tccccctcgg
       61 ctctcagggc ggcgaggaac tggcgaaggt cgtccatcgc cgctgaggtt acgacgccgc
      121 gggccgaagg gctccggccc gcggcggccg ctttactcga agtacccgtt cacgtcgagg
      181 acgacgtgcg tcgtgcctcc ctccggcagg ccggcgcgaa ccgagagcga gccggtcgtg
      241 ccgaggatcg gaatcgcgtt catggcgcgc gtctttccga cggtgaccgg cacctccgtc
      301 gtcccggaga cgatcgggcc tccggcgaag accgagaggg agccggtcgc ggtggggctg
      361 acgacggtga cgttcgcggc gacggagatc gcggtcgagg ggatgccgca gcggccggcg
      421 accgggagaa tcagcgtctg cgcggagccg agggggaccc cgcccgcgac gccgccggga
      481 tcgcgcgtgt cggcgacgcg gcagggagtg acggggtgga agccgagcgg gatcgggagg
      541 gagaagagct cgtcacccgt gacgccgtcg ttggcggcaa agaacatgcg gccgccggag
      601 cgaaccgggg gcgtgcggcc tcctgtcgtg taccaaagtg gcgctccgct cccgatgccc
      661 ggaacgaggt cggcgacctg tgtcgtgccg acctccgtcc cgtcggtccg ccagtactcg
      721 cgtcccgaga cgtgatccga cgcggtgaag agcgcttcgt ggcccatcgt ggagaaggta
      781 ccgacgaagt acgacccgac gggtcccggc gcgacgtcgc ggacgagggt cgtccctgcc
      841 gtcgtcccgt cggtcttcca gggctcgagt ccgtggatgc cgtcgtgcgc gaagaagaag
      901 agggtggagc cgaggaacgt gagggcgccc ggtccagacc tcgcgggacc cgggcagagg
      961 tctttgagaa tcgaggttcc cgcagaggtc ccgtctgccg tccacggctc gaatccgtgg
     1021 gtgccgtcgt cggcccagaa gacgagtcgg tctccgagtc gaccggccac gtacactgac
     1081 gagtcgccgg tcggattgat gtcgcgaaac agcccggtgc ccgccgcggt tccgtcagtt
     1141 ctccagagct cggttccgtg agtcgaatcc gtcgcggaga agtagagggc gccgccgagt
     1201 tccgccgcgg gcccggagga tcctaggccc gccgcgacgg cgaccgttcc cgccgtggtc
     1261 ccatccgttc tccaaagggc ccgcgtggag ccgtcagatg cgtcgaaata gaagtggccg
     1321 ttcagcacgc cgacggcgcc cgggttgctc gatgcggcgc cgggtgcgag gtcgccgaga
     1381 aggaaggttc cgtcggcagt cccgtccgtg cgccagagct cgaggccgtg gacgccgtcg
     1441 tctgcaggaa agaggaggag gtcgcccagc aggccgagga gcggcgaata ctcggcttct
     1501 ggctggacgt ccttgacgag gaaggtgccg gacccggtcc cgtccgtccg gtagacctcg
     1561 aagcccgtcg cggacgtctg agccgtgaag tagacgagcg acggcgtgac gaagaactcg
     1621 ctgggagacg agctgcccgg acccggctcg aggtccttca cgaggaccgt tccttccggt
     1681 gtcccgtcgc tcctccagag ctcccggccg tgttccgcgt cgaaggccga gaacagtacc
     1741 gtgccgttga cgtcggtgag atacgcaggg aaggagtcat tcggcgggaa cacgggctcc
     1801 aggagcgagt aggtgcccga aggtgttccg tcggaacgcc agagaaggtc tgctgtgccc
     1861 ccgatggcga acactcccgc cccaggtatc gagaaggact gatatggcga ggcgtcctcg
     1921 ttgccggcct cgatggcttt caccagcgtc gtccccgccg gcgtcccgtc gctgcgccag
     1981 agctcgaggc cgtcgacgcc gcggtcgacc cagaggagaa gggcaccggg gatcgtcgcc
     2041 gcggtggaga gggagatcga aacgccaaag ccctggacga ggaccgtgcc gccctcggtg
     2101 ccgtcgctcg cccagagctg cgagccgctg gcgctgaaga cacggtcgcc ggaagagacg
     2161 agcggaccgc tgccggtctg cggatggacg agagcggtcc cggcctcggt gccgtcgctc
     2221 ttccagatcg gtccgtcctg cccggcgcgg aagtagagcg cgctgccgtg cgcgacgagg
     2281 ttcgtcgggg aggagctcgc gccaccggga acgatgtcgc ggacgaggac ggtccctgcg
     2341 acggtaccgt cgcttttcca gagctcgatg ccgtgcgtgc cgtcagaggc gccgaagaag
     2401 agcgtcccgt cgacgttcac gggggacgag accgctgaaa ggtccgcgac ctggaacgtc
     2461 ccggcgggcg tcccgtcgct cctccagagg ccgatgcccg gagacgacag cggggggctc
     2521 gcgaagaaga gaaggcctcc cgcctcgacg agggagacgt aggtgttcgg accgatagag
     2581 aacgtcccgt ccggccccgg cgtgacatcc tcgaggagca tcgtcccggc ctcggtcccg
     2641 tcgctcgtcc acggctcgca cccgtggatc ccgtcatcga agaggaagta gaggcgcccc
     2701 cccgcgcgcg tgagccggaa ggggcctccg taggagaacg gatcggcgcc gaccggagtg
     2761 acgaacgtgg tccccgctgc ggtgccgtcc gacttccaga gcgagacacc ccaaggcgac
     2821 cccacgctga agtagaaggt ccccccgacg tcggcgccat agccggtggg gatgtccagg
     2881 agctgccgcg tcccggcggc ggtcccgtcc gtgacccaga gacccatcga cgggtcgtag
     2941 accgaggcgt tcaggaagac cctgccgttc gacaccccga ggatccggtc gaatcccgct
     3001 cgggacgtcg ccgtcgcgga gctccccggg tagatgtcct tcacgagcgt cggggcctgg
     3061 ccgagggcgc cggtcgaggt gaggaccgcc gtcgcaaaga ggagcgcggg aacgcggagg
     3121 ttcatcggga atccgccttt cgtttggggc gcggtgcttc gattcctgcg ctcgcgggtc
     3181 tcactcgaag tacccgctca cgtcgaggat gacgtgtgtc gtgccgccct ccgggaggac
     3241 cgcgcggacg gagagcgagc cggtcgtgcc gaggatcggg atggcgttga gggcgcgggt
     3301 cttcccgacg gtgacgggca cctccgtcgt cccggagacg atcgggcctc cggcgaagac
     3361 ggagagggag ccggtggcgg tggggttgac gaccgtgacg ttcgcggcga cggagatcgc
     3421 ggtcgaaggg atgccgcagc ggccggcgac ggggaggatc agcgtctgcg cgttggcgag
     3481 cgggacaccg cccgcgatgc cgccgggatc gcgcgtgtcg gcgacgcggc atggcgtgac
     3541 ggggtggaag ccgaggggga tcgggagggc ccatagctcg tgacccgacg tgccgtcaga
     3601 ggcggtgaag aagacctttc ctccggagcg cgcgagagtg acgcgattcc cgctgagggt
     3661 ccaggaaacg gtcccggcgg cgagcccgga agcgaggtcg gcgacgagtc tcgtccccgc
     3721 ctccgttccg tcgctccgcc agagctcgcg ccccgagacg tgatccgagg ccgtgaagaa
     3781 gacctcgtgc cccgctgcgg cgaagctctc gaggagcatc gaaccggccg gcccgggtgc
     3841 gacgtcgcgg acgaggaccg tccctgccgg cgtcccgtcg gacttccaga gctcatggcc
     3901 gtggatgccg tcgtacgcga agaagtagag agtcgacccc aggatcgtcc accgaatccc
     3961 gagagacctc gccgggccgg ggttgatgtc cctgaggaga atggtccctg ccgccgttcc
     4021 gtcggtgacc caaggctccg ttccgtgtac gccgtcgtcg gcccagaaga acacccgatc
     4081 acccaggcga cctgcgaagg acggggacga gtctccggac gggttgatgt ccgcgaggag
     4141 ggtcgtgccg gccgcggtgc cgtcggttct ccagagctcg gtgccgtgcg gcgcggcgga
     4201 ggccgagaag accacgacat cgtcgagcac ggcgctcccg ccgttccaga ccggcatcgg
     4261 cccgacgacc gtcgttcccg cggccgtccc gtcggtcttc cagagcgtcg tcgtcgcgtc
     4321 gctcgacgcg gagaagagga acgagcctcc aagggtgccg agcgcgagga ggtcgctcga
     4381 ggcgggaccg ggggtcaggt ccccgagcag gaacgtcccg tccggcgtgc cgtcgctgcg
     4441 ccagagctcg ttgccatgaa tcccgtcgtt cggggcgatg aggagctgct cgcccaggag
     4501 tccgagcatt cggaagtgcc ccaccgacga atctggttgg atgctcttga cgagagccgt
     4561 tccgccctcc gttccatcgg tccggtagag ctgacaaccg gctgaagatg ggcatgcctg
     4621 gaggtagacg atcgacgacg ccgcctggaa ttgcagggga agggaaccgg ccggcccggg
     4681 agcgaggtcc ttcaccatga cggttccttc gggactcccg tcgctcctcc agagctcggc
     4741 tccgtgctcg gcgtccgtgg ccgagaacag cagggtcccg ttcacgtcgg tgagccagac
     4801 cggaacgccg tcgttcggcc ggaagacggg cggctggacc ggggcggtcc ccgcagccgt
     4861 cccgtcggac ctccagagtc cgaagggcat gttgctcagg aggtggagcg acgcgcccgg
     4921 aatcgatacg gagccccggg gagacggact cgcgttgccg ggctcgacga cggtcacgag
     4981 ggtcgttccg gccggcgagc cgtcgctctt ccagagctcg aggccgtcga cgccacggtc
     5041 cacccagagg tgcagggttc cggcgagggt cgtcgagttc cacaggaaga tcgccgttcc
     5101 aaagtcgcgc acgaggaccg ttccggccga cgtcccgtcc gaagcccaga gctgggagcc
     5161 gctcaccgaa tagaggtggc tccccgacac cacgagcgga tcgctcgtcg ggaacgtgtg
     5221 gacgaggacc gtgccgacct cggtgccgtc gctcttccat gtcgagccgt ccgacccgag
     5281 actgaagtag agggcgcccc cgaacgccac gagggcattc ggggaggagc cggcggcgcc
     5341 ggggacgatg tcccggacga ggacggtccc ggcaggcgtc ccgtcgctct tccagagctc
     5401 ggagccgtgc gccgggtctg tcgcccggaa gaagagcgtg ccgttcacat tcacgaggga
     5461 ggagaccgat tcgagatccg cgagctgcgt ggtgccggcc tcggtgccgt cgctcctcca
     5521 gagtccgtag ccgaggtcca ggcggcaggt gaagaagagc gtcccgccga cgtcgacgag
     5581 ctcggaggcg tagtcgagcg agccggtgga cccgggcgtg aggtccttca cgagattcgt
     5641 ccccgccgtc gtcccgtcgc tcgtccagag ttccgcgccg tgtatgccgt cgtccacggc
     5701 gaagaagagc cggctgccga cctttgtggg cttcgagacg tgtgcgatga ccgagggatc
     5761 cgtgctgaac cggctgacga agacggtccc gccggccgtt ccgtccgttt tccagagcga
     5821 tccgccatcc tgggcagccg ctccgaaata gaacgtcccg tcgacatcga cgcccgcgac
     5881 ggggacgagg tccaggatcg cgtgcgtccc cgcggccgtc ccgtccgaga tccagaggcc
     5941 ctggttcccg tcttccattc cgttcaggaa gcccttcccg cccgagaccc cgaggatcct
     6001 ctcgaagccc gcgctcgccg aggccgtgga agacgtcccc gggtagatgt ccttcacgag
     6061 cgtcggggcc tggccgaggg cgccttcaga ggcgaggagg atcgtggcca ggagcgggag
     6121 cggaaggcgg gaacgcatcg cataccccct gcgaccgcgg gcccgtggcg ggctccggcc
     6181 tcgtgtcggc gtggatcgag caattctacg cgccaggggc gcgcgagcgc gcggagaagc
     6241 tgtcgcgcga ggccgctcag ccgtgcgctt cgaggaacgg ctcttccccg ccgtaggacg
     6301 acgagcggct cgcgaggggc ccggggctcc ttcgcagccc gtgtccggac tgctcgatcg
     6361 agtggacgac tccgggagga ggggcgacac ttcgccggcg tgaacgacac ggatacggct
     6421 gcgcggcccc ggagaggagg ggggcgtccc ctccgcctcg cggatgggct cgccgtcgtc
     6481 gtcggagcct tcgtcctatc gctcgcgacg atccgagccg cgagggtccc gatcacgcac
     6541 gacgaggccc tctccttcct ccgcttcgtg tcggcgcccc tcgccgaggt gctctccttc
     6601 gccgggacgg acgccgccaa caaccatctc cttctgacga tcctggaaag gctcctcgtc
     6661 tccgtgctcg ggccttcgga gctcgtcctc cggctaccgg tgctcctctc tttcgccgcg
     6721 ggcctcgcgg cctcctggct gctcctgcgg gggcgctgcg cgccggcgct cgccctcgcg
     6781 gcgttcctca cgttcgccac gaatcggctc ggaatcgagc tcttctcgct cgcgaggggg
     6841 tacggtctgg ccgtagggct ttcggtctgc ggtctcctcg cgctcgtccg ttcgatcgaa
     6901 cgagactttc gtccgcgtcc ggccgtcgtc gggctcacgc tcctggtcct ggccgcgctc
     6961 gcgcagctga ccctcctcga cgtctgggcg gcggcctcgg ccgccttcct cctggtcgcc
     7021 cttcggcggt acgcgacggg accgggaggc gctgcgcggt ccggctggcc gcgcactctc
     7081 ggcgtcgtcg cggtgccggc gctcctctgc gcggcgaccg ccatcccgat ctccttcgcc
     7141 ctgagccgtg ggggcgcgct ctacgccggc ggccgaatgg gattcgtgga cgacacgatc
     7201 ggcacggtcg tgagggagac gctcgcgccg gcgccctggg tagacctcct gcgcgggccg
     7261 gccacggtcg ccctcctcgt ggcggcagcc ttcgtcctgg cggcggtcgg catcctcctc
     7321 ttctcccggg cgaagcgaag cggcgggccc gcggctctct tcgtcggggc gacgttcctc
     7381 ttctacgttc tcgcgatcga ggtgcaggtc cgcgtctttg gggtcggata tcccgtcgat
     7441 cgaatggcgc ttcctctcgt tccgctcctc gcgttcgcga cggctctcgc ggccgcggtc
     7501 ctccttccgg ggggcagcag gctcgtccga cggggagcga gcgctctcgc cgtgctcctc
     7561 tgcgtcgcga gcgtcgcgca gctcgcggcc acggccgacg tcacccgctc gagactctgg
     7621 tggttcgatg cggacgctcc tcacgtcctc gacgagatcg gccgacagac ggcacgctta
     7681 cggccagccg aggggagcgt gagcgtcggc tgctcctgga tcctcgagcc ctctctgaac
     7741 tactaccggg tcgtccgcgg gctgacgtgg ctccttccgt tcgacaggaa gggcctcgcg
     7801 ggagagcgcg acttccatgt ggtggcgacg ccggacatgg cgagcccgga ggccgccggt
     7861 ctcgagacgg tccggacctt cgctgccgcc gggaccgtcc tcgcggtcgc gggagccgag
     7921 aggcttcgct cggcgacgcc accgctcgtc gcgaccggag gcgtcctcga gcccgtcgtt
     7981 cgcgcggcat cgggtcgccg cgaggtcacc gtctggatcc ccgcggtcgt tcatgcctcg
     8041 ggcattggtg gggcgctctg gcgatcggac ctcaggctcc ggaaccgttc ggacgcgccc
     8101 gcgaggatcg tcgtcggcat gagctggaaa ggggagcgcc cccagcgggc cctcgtcgtc
     8161 gccgggagcg gtgaggcgct cgtgccggac gtggccggcc agctcggcgt ggagggagcc
     8221 ggtccgctgg aagtccggat tcggggcgac gtcgaggtct gggcacgcgt gtacgacggc
     8281 gcgactccaa acgacgaagg tgtcgccgcc gggagctggt tctccgcggt ccacgcggcc
     8341 gccgggctcg gacctggagc cgtcggccgg ctggagggac tcgaggaatc cgatcgtcgc
     8401 cgtacgaacc tcggagtcct caacctgggc ccgtcgccgg cggaagtctc cgtgagctgg
     8461 ctgggtcctc gcgggaacgt gctcgtatcc gagagccgga cgctggggcc cgaggagtgg
     8521 cgtcaggaga cgcggcctct cgcgccgcat gccgaagcgg ccccgatctg cggggcgacg
     8581 gctcgagtct ccgtcatccg tggcacgggg atcgtcgcct gggcgacggt gatcgactcc
     8641 gtcacacacg agacccgagt cgtctcagct cgtcgcgagt gatccgaagg ggatctcgga
     8701 ggccgcctcc tccgattccc tgaggcaggc cgtctcggac cgcctgcgcg gtgccgcggc
     8761 gagctcgtcg cggcggcacc gcgggcaccc gtgctcagcc gtgcgcttcg aggaaggccc
     8821 tcaccaccgg gatcgccgtc gaagggatcc ggtggccgcc gtggtggatc tcgtgccggg
     8881 cgctcgcgaa acgggaggcg aggagcgatg cgaacggcgc gacacgctcg agggggtagt
     8941 actcgtcggc gtccgtcgag acatgaagcg cgtgtgtcgc ggaggaggcg gcggtgccgg
     9001 ggccgggctc cgtccactcg cccggcaggc ctccgcagag cgcgacgacg gcgcggaacg
     9061 gatggccgtg cggcggggcg agggcgagcc ggtagtcgaa cgagcagggc tggctgaacg
     9121 cgacgagcga gacgcggaca gggtcggcgc cgcgggcgac gctccaggcg atcgcctcgg
     9181 cgaccgccgc gcggtggacg gcctggttgt cctcgtggcg cgggctgacg ccccagtgga
     9241 agccgcgcgc gccggagggg ccgagcgcct cgccgggaag gaaggccgac tgcggcccct
     9301 cgacggcgac gacgccccac ccctccggcg ccattcgcgc gaggagcggg aagagcgtcg
     9361 cggcgtgcat gccgtagccg tggaggccga ggaggagcgg gaacggcggc ggcgcgtgcg
     9421 gcaggcgcgg gaagagctgg agcgggacgg agatcgaggt cgtcgcgcgc ggtgggatcc
     9481 cgcggcgctc catcatcggg aatccccggg cgggacgatg ctctcgagcg tcgtctcgcg
     9541 cagcgcggag aggagggcgt cgcgggcggg gaggagggcc ggacccgccg gtccggccgg
     9601 gacgtcgagg agccgctcga ggtcgtcggc cccctcgatc gcgaggagga cgtcggcgag
     9661 cgtgatctcg gccgcgggcc gcccgaggac gaagccgccc gtccggcctc gcttcgagcg
     9721 caggaccccg gcgcgggcga gcttctggag gaccttcccg agccacggcg ccggctcgcc
     9781 gcaggcgagc gcgaggtcgc gcgtctcgcg cccgggcgcc cccgggccgc acggatcgag
     9841 atgcgggagc gccttcaggg ccgtctcggc ggcgcgggag aggagcatca gcgtcagcga
     9901 caggcccgcg cggggaacgc cccccgacgg cctcgagcgg gcgtcacgcg ggcctcactc
     9961 cccgacgacg aacgcgtact gctcggccgt cagcgggacg acggagagcc gtccgatcgt
    10021 cacgagaggc gagccggcga agagcttcgc cgccttcatc tccgcgagcg tcacgggccg
    10081 cttcagcggc ttgccggcct tcacgtcgac gaccgttccc ttcgggtcgg ccgggtcggg
    10141 gcgcggcgcg gcggcgacgg tcgcccggcc gacggcggcc ttcacgtcgc ccgtgtggta
    10201 gacgacgagc cggtccccct tcttcatgga gcggaggtgg aggcgcgcct gggcgttcgt
    10261 gacgccgtcc cagacggcgg ccttctcgcg gacgaggtcc tcgaacgagt aggtcgacgg
    10321 ttcggtcttc aggagccagt ccatcaggcg gcgatgatat ccgcgggcgt cccgccgctc
    10381 tcccgcgccg cggcgagctt gccctgccgg cgcccgagcc aggccgagag gagcgcccag
    10441 aggatcgcga ccggcaggaa aaggccggcg agcgtcgcgc cgacggcgcc gagcgcgcgg
    10501 agccccttgt cggcgaaggc gccgacgacg tcgccgaagc ggtagacgaa cgtgtcgacg
    10561 aagctcttcg cggcgtactt ctcctcgcgc gggaggacgg tgaagaggac ctcgcgcgcc
    10621 gggcggaaga gggcgaagtt gccggcgcgc cgcgtcacca tgacgagggc gaggatcccc
    10681 gccgtcgggg agacggcgag cgccgcgaag ccggcgagcg tgaggaccgg aagggcgacg
    10741 agcgtcccgc cgacgccgag caggcggacg acgcggccgg ccaggaagag ctgcgtgaag
    10801 gccgagagga ggttcaccca gaggtcgatc cgggcgaacc aggcggtccg cgcggcggag
    10861 tccgtgaacg tcgccttcac gatccgcgcc tgctcgaagt agaggaacgt cgagctgacg
    10921 gtgaagaaga ggagaaagag ggagatcgcg aggaggtacg tcgagcgcga gacggtcgcg
    10981 aggcccgaga gggcgccgct ccccggcgga actccttccg gctcggctcc cggcgcggcg
    11041 gcgcgcgtcg agtcgtcgac gtggaagtgg cggacgaggc cgcggacgca gaagacggcc
    11101 ccttcgagga ggacggccgc gaggaggatc aggttcgtcg ggccgagcgg accggcgagc
    11161 gaggccgtca acgcggcccc ggcgacggcg ccgagcgttc cgccgacggc gatgaagccg
    11221 aagaggcgct tgccctgctc gggtcggaag aggtcggcga ggaagcccca gaagacagag
    11281 acggcgaaga ggttgaagac gctcgcccag atgaagaagg cctgggccac ggggaggcgc
    11341 cacccctcgg gggcgaacga gaggagggcc cagaagagga cgagcgtccc gaggaggacc
    11401 cggtagacga cggggacgaa gaccttccgc gtcagtcgcg tgacgagctc ggagaacagg
    11461 gggttcaggg ccgcggtccc gaggagcgtc ccgacgaaga gccaggagag ggcgctctcg
    11521 ctgccccgga ccccgaactc gtcacggagg gggcggatga cgtagtagct cgcgaggagg
    11581 aggaagaagt aggcgctcgc caagccgagc gccggcccct cgccctcgcg caggtcgacg
    11641 gcccgggcga ggagggcgcg gaggcgcgcc acgtcagccc ttcgccttct tctcgcgaac
    11701 ggtggcgagg gcggcggtct cccgctcggc ggtcaggccg gccttcagcc gcgagcgtcg
    11761 ctcctcgggc agggtcttcc accaggcgag ggtgtcccgc gccgtctcga cgatcgggcg
    11821 gaacgtgagc ccttctccca ccgccttccc gttgttcacg cggcgggagc cggccgtctc
    11881 gccgaagggc ggaacccagc agggcatgtc gctccagggg gcgatcttca gctcctcgag
    11941 cgtcttcgcg tcgacccagg tgaaggtcgc gttcgagccc gaggcttcgc ggcaggcggc
    12001 gagcatcggc ccgaacgtca gcggctcctt cggacccgtg gcgttgaaga cgccggtccg
    12061 ccccttgccg gccatcgcga tcgtccacgc cgcgaggtcg cggacgtcga cgaactggac
    12121 cgggtcctcg ggggtcccgg gagcgaggac ctcgcccccg cgggcgacgc ggaccggcca
    12181 gtaggtgaag cggtcgctcg tgtcgccggg gccgatgatg aggccggggc ggatcacgag
    12241 ggccttcccc ggcattgccc tctcggcggc ctcctcgcag agcgccttca gcgccccgta
    12301 gttcgcgccg gtcaccttgt cggcgtcggg ctggtcggtc ttcgcgacgg gggagcgctc
    12361 gtcgatcccg ggcttcgacg tgtcgccgta gaccgagatc gtcgagacga agacgtagag
    12421 gccgacgctc ttcgcgagga gcctcgccga ggccgtcacg tccttcggga agtagccgga
    12481 agtgtcgacg acggcgtccc aggagcggcc ctcgagggcc ttcaggtcgc tccggcggtc
    12541 gccgcggagc ttctcgacgt cggggaagag gtgagggttc gtcttcccgc ggttgaagag
    12601 cgtgacggtc cagcgggcgc tctgcgcggc ctcgacgagc tcggggccga ggaaggccgt
    12661 cccgccgagg atgaggagct tcctccgggc cggaggctcc gctccgagag cgtcgagggc
    12721 cgtcccggcg aggccgaaag ccgcgcccgc cgcggcgccg gtcctgagga agtccctccg
    12781 cgtcgttccc cgggcgattc gagacatgtc actcctcctc ggacgcgccc cgcgcgggcc
    12841 gccgcgggtg cggcgccgag tcggaggtac ggcccgcggc aggagcgggt ttccgccgga
    12901 gggtcagccc gcctcgcgga ggagctcgac gaccgggcgg cgcgcgaggt tgagggccgg
    12961 cgcgagaccg gcgaagagcg ccaggagcat cgacacgagg gccgtgaaga agagcgccgg
    13021 agcgtcgggg acaaggagct gtcccatccc ggcgtattgc gtcttgtcga gcgccgcgcc
    13081 gatgccgcgg gcgaagagcc acatcacgcc tcccccgagg agcgccccgg cgaggccgta
    13141 gacgagcgcc tcgccgagga ggagcccggc gatcgccttc ctcgggaaac cgaggacgcg
    13201 gaggacggcg gattcgttcc gccgctcgcg ggcgctcatc gcgaggctgt tcgcggtgat
    13261 caggaggagg gcgaagccgg tggcgacgcc gacgcttccg agcagcgcgt tcacgttccc
    13321 gagcatctcg aggaactgga gctgccactg ccgctccgtc atcgcgagga ccgggacggg
    13381 ggcgttctcg agggcgcggg cgagggccgc ggtgaccctg tcgacgtcgc cgcgactctt
    13441 cgcgaggacc cagaacatgt tcgtccgccc ctcgtcggcg gtgagctgcg agaagtagcg
    13501 tcggtggaga tagagcccgg cgtcgaggcg atagcccatg acggcgcgga tcgtcgtctc
    13561 gacgaccccg ccggggaccg gcgcttgcag gacaacccgg tcgccgacct tccacccgag
    13621 cttctcggcg aggagcggcc cgacgagcgc gcccgtcggg tcgtcgaccc acgccttcgc
    13681 ctggtcggcc ggcacctcgg cctccatgta gacgcggagc atcggttccg ggggcgccga
    13741 ctggatcggg acctggttct cggggcgggc atcccggtag aagccgacga ggaagtcgaa
    13801 cgggacgacg gtgtcgaccc cgggcgtcgc ctcgatcttc gccatgtagg acatcgggag
    13861 cttttcgaag aaggagctct tcgccgtcac gatcatccgc tgcgccatgt ggggcgacca
    13921 ctcctttcgg agggccgacg acaggagcag caggaagccg aggaggccga tggagaacgc
    13981 gaacgctccg gccgtgaggg acgcccgcag gggcctcgcg cggaggtgac ggaggacgta
    14041 gccggcgaag gtcaacgagt gagtctacgt cgggccggcc gccgggtttc ccgccccgga
    14101 gaccttcgcg aggcgcccgt ccgctatcat gcccgatcgc ggcccgatgc gttccctcct
    14161 cgtccccctc ctcgccgtcc cggcgctcct cgcggccccg ccgacggccg acgtgtctcc
    14221 cctccgtatc acggagctga gcgtctgccg ggagatcgtc gaccgggggt gcgagagcga
    14281 ggggcggacg ttcggacccg acgtggagag cgtcgccgtc ctgacgaggg tggagggggc
    14341 gaccggcgag gcgttcgtct tccacgtctg gtccttcgag gcgaaggagg tccagcgcgt
    14401 tcgtctcccc gtgcgcgcgg ccgtctaccg gacctggtcg aagaagagcg tgaaggggca
    14461 gccgggacgc tggaaggtgg aggtcctcga cccgatcggg cgttccctcg gcgtcgtcga
    14521 cttcgccgtc cagcccccga agatcgactg acccgtcccg gtcttcccgg tcgtcgcggg
    14581 ctccgccgtg cgccttgacg ttcgccgagc cccccggcta ccgtgccgga ggccgggagg
    14641 ctagctcagc ggcagagcgc ctgctttaca cgcaggtggt cgggggttcg atcccctcgc
    14701 ctcccaccac gacgcttcag ccccgtcccg cctccgcgag cgcctcggcg agctgtgtgc
    14761 cgctcatccg gagcgcgcgc gcgaagccgc cgacccagcg ggcctcggag ggttcgaacc
    14821 ggaagaggac gaagtcggcc agcgcgaagc gctccccggc ggcggggaaa cgggagacgt
    14881 aggcggcctg ccccgtctcg tactccggcg tcccgggcac gagcgcgagt gccacccccg
    14941 agagagagag cctcgggagg gtctgggggt cgccgtctcc cgtgtcggga gccgagacgg
    15001 cgagcgcggc gcgcggctcc gtcagcaggg ctcgggtgtg gagcgccatc tgggagaggt
    15061 ggaggtagag cgaagcgccg tcgggcgcga gggcgtagga ggtcatcgcg gcggcggggg
    15121 cgccgtcgac gacgacggcg agcgctgccc agcggctgga gaggaggagc cgggcgggag
    15181 ggaggtacgt ttcggagacc atcggcccct catcagccgc gcagcgcgcg gaagtagtcg
    15241 atcgtcgcgg cgagcccgtc ctcgaggctc accttcggct cccagccgag cttcgccttc
    15301 gcgagcgaga tgtcggggcg acgctgcttc gggtcgtcga cgggcagcgg cttgtggacg
    15361 atcgtcgact tcgtcccggt gagccggcga atcgtctcgg caaactgcat caccgtcatc
    15421 tcggccgggt tcccgacgtt gcaggggtcg gactggtcgg agaggaggag ccggtagatg
    15481 ccgtcgacga ggtcggagac gaagcagaag gagcgcgtct gggagccgtc cccgaagacg
    15541 gtcagcggct cgcccgcgag ggcctggttg atgagggacg ggacgatccg gccgtcgttc
    15601 gcccgcatcc gcggaccgta ggtgttgaag atcctcacga tccgcgtctc gacgccgtgg
    15661 aagttccggt aggcgacggt catcgcctcg gcgaaccgct tcgcctcgtc gtagcagccg
    15721 cgtggcccga cggggttcac gttcccccag taggtctcgg gctgggggtg gacgagcggg
    15781 tcgccgtaga cctcggacgt cgaggcgagg aggaaccgtg ccttcttcgc gtgagagagg
    15841 ccgagcgcct tgtgcgtgcc gagcgcgccg accttgagcg tctggatcgg gatctggagg
    15901 tagtcgatcg gcgacgcggg cgaggcgaag tggaggacgg cgtcgagagg gccgtcgagg
    15961 tagatgtact cggtcacgtc gtggtggagg aaccggaagg cccggttccc catcaggtgg
    16021 gcgatgttcc ggacgtggcc ggtgatcagg ttgtcgatcc cgacgacctc gtgcccctcg
    16081 gcgaggagac ggtcggcgag gtggctcccg aggaatccgg cggcgccggt cacgacgatg
    16141 cgcatgcaac ccatcattct acgggatcga tcgaagggga gacggaagcc cgcaaggagc
    16201 acaataaacc gttcggagga gccccgatgc cggggctcgg ggaggacggc atgagagtgg
    16261 tgacgctccg ctcgctcgtc ggtttcctgg tcctggtctt cgccgtggcc ggcccgctcg
    16321 cggccgaccc gccgaccctc gaggggacct ggctcttcga cgcgagggcg tcgaagaacg
    16381 tcccggaggc gctgaagggg gtcgacctga agatcgacgt gaaggacccc caggtgacga
    16441 tcacgaagct cgccggggag cgccccgtcg gcgagccgat ggtcgtgatg ctggacggga
    16501 agtcccgccc gcaggacgtc ggcggccatc gcgccacggt gaccgcgaag tggctcgaga
    16561 aggggcgccg gttcgagcac gtcatcagca tgatgcaggc ggggagcgtc ttcgcgacga
    16621 cgcagacgat tgtgaccgag gtctcggaga ccggcggcac gatgctgcgg aagtactcca
    16681 tccggcaggc gcgcgagacg caggagcgcg cgctcgtcta tcgccgcaag ccctgacgcc
    16741 cgggcggggg cgacggggaa aggaccgcgg ggccgccgcg accgcgttcg atcccggacg
    16801 gaacgctcag gcccggccga tggccacgta ctcccagccg aggcgcttca tctcgcgcgg
    16861 ctcgtagagg ttccgaaggt cgacgaagcg cttcgccttc atcgtcgcct cgaggcgcgc
    16921 gaggtcgagc ttgcggaact ggttccagtc ggtggcgagg accaaggcgt cggccccggc
    16981 cgcgcacgcg tagacgtctt ccgcgtactc gatgcccggg aggacggcct tcgtgttctc
    17041 catcgccgcc gggtcgtagg ccgcgacgga ggcgccgcgg gccacgaggt cctcgatcag
    17101 cttgagggac gagctgtcgc ggatgtcgtc ggtctccggc ttgaaggcga ggccgaggac
    17161 ggcgacccgc ttcccggcga ggtcgccgca gaccttctcg accttctcga ccatccgagc
    17221 cttcaccgtc gcgttcacgc ggaggacggt ctccatgatc tcgaacgtgt agccgtgctt
    17281 gcgggcgacg tcgacgacgc cggaggcgtc cttcgggaag caggagccgc cgaagcccgg
    17341 cccggggagg aggaagtggg gggagatccg cttgtcgagg cccatccccc gggcgacgtc
    17401 cttcacgtcg gcccccatcc gctcgcacat cgcggcgatc tcgttgatga aggagatctt
    17461 cagcgcgagg aatccgttcg aggcgtactt gatcagctcg gccgactcga cgtcggagac
    17521 gacgaacggg acgcccgcca cgatgagcgg ggagtagacg tccttgacga tggcgatcgc
    17581 ctcggcgtcg cgcgagccga tgacgacccg gtcgggacgc atgaagtcct cgatggccga
    17641 cccttcccgg aggaactcgg ggttcgagac gaccgagaac ttgtgtccgc cggggttctt
    17701 ccgaaggatc tcctcgatct gcgcgcccgt gccggtcggg accgtcgact tcgtgacgac
    17761 gaccttgtag ccgttcatgt gctccgagat cgcctcggcg acctggaaga tgaacgaaag
    17821 gtcgggggag ccgtccgcgc gcgggggagt ccccaccgcg atgaagatga cgagggcgcg
    17881 ctcgaccgcc tcgcggatct cggtggcgaa gtggagccgc ttggcctgcg cgttcttgtg
    17941 gatcagctct tcgaggccgg gctcgtagat cggcgagacc cccttcttca ggagggcgat
    18001 cttcgtctcg tccttgtcga tgcaggtgac gtccatcccg aagtcggcga ggcaggcgcc
    18061 cgtcacgagg cccacgtatc ccgttccgac catgcagatg ttcatgcgtt cctccaacag
    18121 gggactccca ccgggcggtg tccggacggc ggagtctagc gtcggagagg ggaacctgtc
    18181 ttcagcgcga gcgcccgccg ccgagcgggc cgcggaagaa gccgaaaagc tccctccggg
    18241 agacctcccg gggcgcgtcg ggcgcggttg cggcggctgg ggcgggccag accggccgga
    18301 gcgcggggcc ggggcggctc gtggggatct ggggagagcc gaggcgggcg gtgacgatct
    18361 cggtgagggc cgtctcgagg ggcgtggggg gccggaagcg gaggagttcg cccgcgagcg
    18421 cgaggtcggc gatccggccg gggcggaagg tctcgccgga gaccctgtag cggacggggc
    18481 gggacgcgcg gtcacccagg aggtggagga cgaacgaggg ggcgacgcca cgcccgctcc
    18541 cgaggttgat cgttcgcccc tccggccgga gggcgagcgc ggcgaggatc ccgtggaccg
    18601 cgtcgccgac gtggaggagg tcgcgcggcg cgtcggaggg gaggacgatc tcctcccccg
    18661 cgaggagcga ctcgagggcg tcctgcggga agcgggtcgg cggcgctccc ggggcgagga
    18721 ggccgaagag acggaggacg acccacgaca ggtacgaggc gctgaggcgc gactcctcga
    18781 ccatgcgctg caggagaacg gggtcgcgcc cggggtcgat cggctcggcc tcggtcgccg
    18841 gctggcccgc ctcgcccggg gggccgtaga gcgcggcgtc ggagaggtgg acgaggaacg
    18901 gcttctcgtg cgcgcgtgcc gcctcgagca tcgcgtcgag gcccggcccg ccgggaggga
    18961 agagcgccgc gttcacgacg gccccgggcc gggcctcacg gaagacctcg gccgcggcgc
    19021 cggggagcga gaggtcggcc gtgacgacct tcgcgttcgg gtaggcgcgg agcgtctcga
    19081 gccgggcctc ccggacggcc cggccgtccc cctccgcgac gagggcgtcg acgaccgtca
    19141 cgtcggcccc ctcctcgagg agctgccgcg cgacggccga gccgacgagg ccggcgccgc
    19201 cggcgacgag gacgcggaag gccaccgcta ctccctcggc tccggagtcg gcgccgggcc
    19261 gagcgcctcc ggcgcggggg ccgcgccctt cgcggtcgtc tcctcgtagc gtgcctggat
    19321 ccccgagcgc tgccggttcc tcacgagcca ccggaggagc ttcgactcgt tcacgccgac
    19381 gaggatgtcg tcgatcgcct cgaagagggc cgggtcgttc acgatccgcg ccgccgtccc
    19441 ctcgccccgg tcgaccttct cggcgacgct cgcgaggctc gaggaaatgc gccgcaggtc
    19501 ctcggtcagc tccttcgcga acgcctcgtc gcggacgagc ttcggcagga ggccggagcc
    19561 ggaggagagc tcccgcgaga agcccgagag gccctccgcc gtcgcgttca gggagtcgac
    19621 catcgcgaag aacttcttct tcccctccgg gtcgccgaga agcgcgggga cgagcccctc
    19681 cccgtcgccc gcgcccctct cgacggcggc gaggagcttt cggagcgagg cggcggaggc
    19741 cgcgagctcg gcggcgacct cgtcgccgag cttctcgtcg gcgacgagcc ggccgacgag
    19801 ccccttcccg gcccgcacgt cggcgagaag ggcgttcgtc gcggcgatcg tctgccggag
    19861 gctccgcgag agctccttcc cttccggaga ggcgctcgtc atctcgccga ggaacccctc
    19921 ccccttctcg acccgtccga ggatcgtctt gagcgactcg gcgacggagt tcacgttctc
    19981 gaagaggtcc ccggagccgg cgaggatctt gtcgagctcg ctccctcgga tcgccgcgat
    20041 ctccgacccc ggatcgagga ggggcgtacc gggggagccg ggcgtcacct cgaggtactt
    20101 gtcgccgagg aggccgatcg tcttgatctc cacgcggctg tcggtcctca cgaggtgccg
    20161 gtagcggcgc tcgatgtcga agcggacggt gaggtcctcg ccggggctct tcggcacctc
    20221 gacctcctgg accgcgccga cggtgacgcc cgcgagccgg acctggttcc ctccggagag
    20281 cccctgcgag ttcgggaagc gcgcgaagta gctggagcgg gcgaggaaga agccggtctt
    20341 tccaccgacg acgacgacca tgacggcgaa gaggaggatc gcggcgaaga cgatgacccc
    20401 gacgcgggaa cgggtgcggc tgtcggcttt cacggtgcga cggtctccct caggctccgg
    20461 cctcctccgc ggcgaggaac gcgcggaggg cggcgacgtc ggagctccgc gcctcgccgg
    20521 gggtcccgac gaaggcgaag cgccccttct cgaggaacgc gatccggtcg gccacgaaaa
    20581 gggccgaggc gatgtcgtgg gtgacgacga cggaggtcgt cgcgaggcgc gagttcaggt
    20641 cgacgatgag gcggttgatc gtcatcgccg tcacgggatc gaggcccgtc gtcggctcgt
    20701 cgtagaggag gacgtcgggc ttgagcgcga tcgtccgggc gagggagacg cgcttcttca
    20761 tcccgccgga gagcgaggac gggaggagcg gcaggacgtc ggggccgagg tcgacgaagc
    20821 cgaggacctc cgtcacccgc gcggcgatct cctcgccggt catcttcgtg tgctcgaaga
    20881 gggcgaaggc gacgttctcg aagaccgtca tcgagtcgaa gagcgccccc atctggaaga
    20941 gcatcccgac cttccggcgg acctccccga gctcctcctc cgcgaggcgc gacacctcga
    21001 cgccctcgac gaagaccgac cccgcgtcgg gctgggtgag gccgttcatg tgccggagcg
    21061 tgaccgactt gccgcttccc gagccgccca ggatgacgag gacctcgccc cgcgcgaccg
    21121 cgaggtcgac ggcgtcgagg acgaccttct ctccgaaggc cttcgagaca gcgcggagct
    21181 cgacgacggg ctcgctcatc cgatcggcag cgcgatgaag agcttggaga ggaagaagtc
    21241 ggtgatgagg atcgtgatcg aggcgacgac gacggcgacg gtcgtcgcgc ggccgacgcc
    21301 gtcggcgccg ccggtggccg agaggccgtt gtagcagccg atgagggcga tctcgaggcc
    21361 gaagaacggc gtcttgcaga ggccgtgcat caggtcgttg aacgagagcg cctgcagggt
    21421 cgtcgagagg tagaagctcg cccctatctt cagctcgaag acggcgatga agagccctcc
    21481 gagcatcccg tggaagatcg cgatgagcgt caggaccggg agcatgaccg tcagcgcgac
    21541 gacgcgcggg aggacgagct tgcggatcgg ggaggagccg agggcccgca gggcgtcgac
    21601 ctgctccgtg acggccatcg tcccgagctc ggccgtgatc ccggcgccgc agcgggcggc
    21661 gagcatgagg gcggtcagga cggggccgag ctcgcgggcg agggagagga tgaccgcgcg
    21721 gccgacgaag agcttcccgc cgaactcggc gagggagtgg gcggtctgga gggcgaggac
    21781 catcccggtg aagaggcagg tcaggttcgc gaccgagatc gactggacgc cgagggcgtc
    21841 catctgccgg aggatctcgc ccccttccca ccgcctccgc ccctcgcgca cgatcgacca
    21901 tgcgaggagg ccgaggcccc cgaggtggga gacgaccttc cggaaccagc ggcggagccg
    21961 cgggagggtc cagaaggggg ccggccggcc gtcgggtgcg gacgcttcgg acacgcgggc
    22021 gattgtacgg gcggcccggt agacttccgc tccgatgcag ctcctctaca cgacgtgggt
    22081 gtggctcttc gggctcctcg tcactcccgt gttcggcacg ctcgccatcc tcacctcctg
    22141 gatcccgccg cggggccgcg tctaccagta ctgggcccgc tggtggtccc ggacattcct
    22201 ggccggggcg ggaatcgacg tgagggtgga gacgtccgag gccgcgcgca cggtccctga
    22261 ggcgatcttc atggcgaacc acacctcggc ggccgacatc gtcgtcctct tcgtcgcgct
    22321 cgcgcgggac gtccgcttcg tcgcgaagaa gcagctcttc tggatcccct tcctcggctg
    22381 gtcaatgtgg ctcgccggct tcatccccgt cgaccgcgag aacaaggaga aggcgcgcga
    22441 ggcgttcgac cagatcggga agcagctgag gaagggcgcc tcgatcctcg tcttccccga
    22501 aggaacccgc tcgcggaacg ggaagctcgg ggcgttcaag aaggcggggt tcctcctcgc
    22561 gatgcggacc tctctcccga tcgtcccggt cggcatctcc ggggcgcggg cggtgctcgg
    22621 cgcggacggg ctgctcgtcc ggcgggggcg catcgaggtg agggtcggcg acccgatccc
    22681 ctcggacggc tacacgttcg cccggcgggc ggaatacgtc gagagagtgc gggccgagat
    22741 cctccggctc ggcgattcgg cggggtaaga tccgccggcc gcgcggcgcc gggcgacggg
    22801 cccgcggagg ccggccgccc cgccggccac atcgatcgga ggtccaccgt gtcgaacctg
    22861 aacgaggacg ccgtcctcga agcgctccgg aaggtcaagt accccggctt ctcccgggac
    22921 atcgtctcct tcggcttcgt gaaggacgtg gccgtcggcg gcgggaacgt ctcgttccgg
    22981 ctctcgatga cgacggcctc cgcggagatc gccgagcaga tccgggtcga ggcggacgcg
    23041 gcgctccgga cgctcccggg ggtctcggcg gtcaccatca aggtcgagac ggggcaggcg
    23101 ccggtgaacc tctcaggggc gcagggcgcg cgccccgacg tcctccccga gacgcgcttc
    23161 acggtggccg tcgcctcggg gaaggggggc gtcggcaagt cgacggtggc cgcgaacctc
    23221 gccctggcgc tccggcagct cggctacacg gttggcctcc tcgactgcga catgtacggc
    23281 ccgtcgcagc agatgatgat ggggatcgac gagaagccgt tcctcaacga cgagg
//
LOCUS       DAOONL010000053        23193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2119_length_23193_cov_13.889532, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000053 DAOONL010000000
VERSION     DAOONL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_23193_cov_13.889532"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1668)
                     /locus_tag="PLP50_09595"
     CDS             complement(46..1668)
                     /locus_tag="PLP50_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HPA51845.1"
                     /translation="MPASLVSLLLRSARENPGRAAVGLPGEEPLTYSGLDRASASVAA
                     WLAERGVRRGDRVGLLLPKSVEAVVGVWGALRTGAAYVPIDRSSPPQRAALIASNCGM
                     SALLASTEVPDAVEAIRAALPGIPVLLLHGDGGAGVPPGPAGPLPSPNVRDLAYILYT
                     SGSTGVPKGVMVSHGAALSFVEWAAARFELRPEDVLSSHAPLHFDLSTFDLFGAALAG
                     ARLVVLDEETVRFPMASAEVLETERISVWYSVPGALRRMVRTARLSDRKLPALRTVLF
                     AGEVYPADELRALQLALPPDTALYNLYGPTETNVCTYWRVPPAGTWTSPAVPIGIDCE
                     NCEGVVVDPELRPVPEGETGELLVRGGTLMEGYWGDAEATARSFVADFLHPHLSDRLY
                     RTGDIVRRQPDGTYAFHGRVDHMVKVRGYRVELGEVESALLRAAGVTEAAVVAVEREG
                     SSGRETELVAFVSGEGPDPSRERDLRRHLASHVLKYMVPAEIRFLAAFPTTSSGKIDR
                     QALKTLADRPEPASSGAPASRTQSGSRPGGTT"
     gene            complement(1671..2816)
                     /locus_tag="PLP50_09600"
     CDS             complement(1671..2816)
                     /locus_tag="PLP50_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA51846.1"
                     /translation="MDFDFTSDQVELREAVTAFARAELGADLLEEEKAGHFSRTKWEA
                     AARFGLQGLPMPEAFGGTDRDLLTTVCAMEGLGYGCPDNGLIFSLNAHVWSAEMPILK
                     FGTPEQKDRYLPKLVGGEWIAVHAMTEEGSGSDAFNVRTTATRRGDRFVLNGSKLFIT
                     NAPVADVVVVFASEDLSSGPDGISAFLVEKGTPGFSVSRPFEKMGLRTSPMGQIFLED
                     CEVPAERRLGGEGAGVALFNSSMDYERACILAANIGTLERQLETCVERARTWQRFGKA
                     IGKFQSVSNKIADMKVRLETARLLLYKTAWLKGQGKRASMESAMAKLYVSECFVQSSL
                     DAIELFGAYGYMAEYEIERDLRDAVGGTIYSGTSAIQRMIVARHLGL"
     gene            complement(2833..3468)
                     /locus_tag="PLP50_09605"
     CDS             complement(2833..3468)
                     /locus_tag="PLP50_09605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPA51847.1"
                     /translation="MSGKAPLLPDALASSLGHAGRVRVQDSLGDRLKQLARNALLFGA
                     NRVVGPLPGHALRLAYYRNALGWRIGERSSVHHGLKIFGGRGRVTIGNRSTFQMDCLI
                     VGAGMNDLTIGDDVAIAYRVNLVMGSHDVFSPAFDGITGPITMEDHVFVGTGSTILLG
                     VTLGEGTVVTAGSVVTKSSPPYSILRGNPAQVVAKRPRNLSYSAEHFWTFH"
     gene            complement(3465..4982)
                     /locus_tag="PLP50_09610"
     CDS             complement(3465..4982)
                     /locus_tag="PLP50_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HPA51848.1"
                     /translation="MSDSIHPVGSATPAPPAAAQPGDLAEALLRLARSVTSRRRLFAA
                     VALGTFLAIQVFAFVWPATYEARAAVMIQKSRYTPRLDSPARAESPVLVAEITEEEVN
                     SEIAVLTSRRVLSATVEATGLAKARPSWFWRLLFAPLRAYERAHAAYHEVTPPNDVDR
                     AIRALSGSLGVERLKESRILVVTYEAGDPGVAEAVLAELLRNYVAHHVEVHGTQRVVP
                     FFSSQTETLARELARHEDALQAIKKRVRAVDMPTEREVQLKLDAGLREENEMLGRRLV
                     ELDGKIAAYDRLLERAARGETDSAASAASQPALDEFKVQALRLQLEQIRVEGRYQDDA
                     PPVLENRRKLEAARKALEEERQYAAQHGPTAVALREERERAVAERAGIVERRQALERQ
                     LRTSRARLVELDEGSVAASRAQRLIRSVEDRYMMVLSQQERALIDSALDSGKFTNVSV
                     VQEAAASAKPVRPKKLIVLLASVAGGLLLGFAACVAQEVRVTGLAPLLAAAVREP"
     gene            complement(4979..6151)
                     /locus_tag="PLP50_09615"
     CDS             complement(4979..6151)
                     /locus_tag="PLP50_09615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA51849.1"
                     /translation="MYYPWLYLTSGAERTILEIAKRSRYRITIFTNEYQPDATFPELG
                     QMDVRLLSRVPVERSLRSVASAATRLLSQELDLSEFDGLLVVCEGLGDLVALRQRRIP
                     VFCLCLTPLRIAFDPVYRANYLASRNALHRVVVRAGTFLYRGVDRIAWRRYRRVFAIS
                     EEVRTRILAGRLAPPERMRTLHPGVELSAFTPSPPAERVFFVPGRIMWTKNLELAIQA
                     FQLFKETAPEPSTWKLVIAGIVDRKSRPYLERLRSLAGGDPSVEFRIHPSDEEMRALY
                     AACFATLFTAFNEDWGLVIIEAMASGKPAVAVNRGGPKEIVRHGHDGLLSEADPVAFA
                     SAMARLAGENGLHERLAMNAPSSAARFGWAPFVSELDDELERTLGALDAAGAREAA"
     gene            complement(6243..>7466)
                     /locus_tag="PLP50_09620"
     CDS             complement(6243..>7466)
                     /locus_tag="PLP50_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPA51850.1"
                     /translation="HSRRPKLSSGYLGEFYSPVVGPDEFIKLATALKAVPFVTANTGT
                     GSAEEAAAFARFFESRGFPVVYWEVGNEVYFEGLSESGQIGLPPDVYARRVIEYAAAI
                     RKQAPYAKIYSAAVIGPEEQDSFWNSVVLGLAGPYVDGISVHNAYFPLYGYRADGTIP
                     PDDELFTSMLGATKAVERSMTVLEGQLRRLGKLIPIFVTEYDGIFYPDEAIEDPVRTH
                     QRNPTLASALFNASVLHVMARSERVYGAHHMSLAGTHFGSLIGTAGAARFRNPQFYVH
                     REYARESGNLMTEVRLDPKDALFDSKPVKLLSGQMDVPMLDALATRDPGESHFALFVV
                     NRSLSTAVDATIEMNLPSGVTGTVSVLNGPGPGARNSAERPDAVALTTTPFGAAGAFS
                     YRFPPHSLTIFRFAR"
     assembly_gap    7467..7566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7567..7975)
                     /locus_tag="PLP50_09625"
     CDS             complement(<7567..7975)
                     /locus_tag="PLP50_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51851.1"
                     /translation="MRKTPPLSHRSWISRIARAANAAALLVVFAAARPARAQPMPVAT
                     SSIHVDFQKVKRVIPALLFGQNLQTIERGEGIVKPDGSFDREIVSLLSEARVTTLRFP
                     GGTAADFFHWWQALGPHSRRPKLSSGYLGEFYSP"
     gene            complement(7981..12108)
                     /locus_tag="PLP50_09630"
     CDS             complement(7981..12108)
                     /locus_tag="PLP50_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51852.1"
                     /translation="MLQTLPWRRLALAGLFVVFPAARAQEPGWEELGPSSVGGRVSAL
                     AADPRDPRHLVVGTPAGGLWRSTDGGQVWQTLAPWLAATPLSAVAISPVDSNVLLAGT
                     GALSDGGSVSPGIGLIRSTDGGTSWTVAGPIGAGLYVSALLVDDADPSRVLVATDTGV
                     KLSTDAGTTFAATLDGDAVSMLVRDPLRAGSVFASGRGGLYRSDDRGSTWVRTAGWPL
                     RATDTFGVGTTSVAVSARTPGLLYATIQVLGDLNRTDRALLLRSRDGGTTFEELAPPP
                     FCTSCGFAQAIALDPLDDARILLGGDGLSVSTDAGATWRAVTGDAAGVHRILVLADRV
                     LVAGTSGVATLDAAWSTATSSSYGLAVTSITSLDASRESPPRLLAGTADSGTLLAVPA
                     DPVPVWHVIHGRREAAGPARFDPFDADRLYVSSTGGRLFRSDDRGVSFRPIVQGLDLD
                     QPAPTEAPLEPSPLDPGTLFTGRMQLFRSTDSGDSWTGFRPPGSPEITRIAVSPVRSG
                     RVYFAVALGAKLFKADGIGTDLITISDDPGLAVTSLHLDPDAENVLYATLADPSSQTG
                     RVFKSYDFGQAWEEISLPGLATPSSIVKDAFGALYIGTSDGVWRSPNDGSSWSAFRNG
                     LAGAGVSALRVADDTLFAGTFGRGVFRIPLEELVTIDTIPSGLEILVDGQVVTTPYYA
                     RWARGSVHRIEPVLFENEDTRQVFVSWSDGGASSHPVTTTTGREWFTAAIRRSHRLRT
                     EVTPTSGGSLALDPPSADGFYVERSFATLVAIPSPVHRFAGYSGDVSGSDGILGFAVM
                     DRPRSVKAHFEPLHLVVKSEPSGLALGVDGASSTTPATFQWASGSVHVVSAPELVDRG
                     EGSRPLAFDGWSDLGDREHSVVVHRDTFVTDLTARYIPTITTLAVPASGSAFLKTTGQ
                     DDAPRLAALLLTPATGAPAPEALQVLSGRLDGTVVTELATVSSEPRLLSHAYVEEGGT
                     AGRTRITAYNPGTAEASVDLVLRGLDGTPIQTTSAAFRVPAGGFTVQFLSDLLAFPEQ
                     VRGLLTLVSNRPLATSVHSMRANLRPSSFLDPVLVAPFNDADSGVPSSAAVQSILLTP
                     DTVHRLAVSNTGLAGLSGTLAFRDDTGAPLRVELESGHATTAGYELPPGGTAVFRFRV
                     PDARPSDAEVRTAQVRVLPRNDQAVGALAPRPSASQPAPMVQLVEERTVGNTSGQPVV
                     LPRTVPPSRAVTRFLVPVELALRDSGVILTNRLGLAVTATLTLRDSGGGTVGSADVPV
                     PAGGQVAISCRSLFPSAGDLQGSLAGTVAGPNGATLDVVGFLRRVNDRGEEILAGFPV
                     LDGAAGTRDLAPVFPLALDGDSWRSEWSLWNATPADLVTRLVFQRDGRTAYFPFE"
     gene            complement(12189..13868)
                     /locus_tag="PLP50_09635"
     CDS             complement(12189..13868)
                     /locus_tag="PLP50_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51853.1"
                     /translation="MKRAVLLLPMLTVLVAGCGKNRASASGSGVVAAAPQTPPAAPER
                     AKVTIDATRVLRLVSPLLFGMHIEWVENGNGLLEADRPALRSEVLELLQPLRVPAFRF
                     PGGIHADYYDWSLGIAGPNHRGSSRNAFNGQSLEHRFGTPELVALLEKTGAEAVLTAN
                     YGTGSAEQASAWAKHLRSLGARARLWEVGNEIYLTGPREGGPNGKAIYRRPEEYAKDF
                     PRYRSALRAVFPDALVGAIGHLDTGAFPLAPEESRDWTVRMLESLEGPVDFLAVHNAY
                     APVILDDSASFADERARGEVYRSLYAGAAETRENLDQVLETLARLSPANANVPLAVTE
                     FGPLFGVSSRRDQHAIYVDQSRTLAAALYVASLLDVFLGEPRIFLACYTNPIHRWYGS
                     LLTDTEAGLVRTPTYHLYSLYRSRFEPGLLSTVVTSPTFDARANGIVKAHSGLPDLLA
                     RASLSADGSRLTALLVNRSLDRPLRTAVSMAGFAPRSTDCLLLTAPSPASINGPGLTT
                     TTRTGGEEIAPRPWPCPAGNPLEVEIPPNAILSLVAEQAPARTTSVAPPRK"
     gene            complement(13865..14971)
                     /locus_tag="PLP50_09640"
     CDS             complement(13865..14971)
                     /locus_tag="PLP50_09640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPA51854.1"
                     /translation="MRVLQVIDKSFLGGGQTVVRHLLEGFRGTDVESLLACRDGGPLV
                     EAARALGTRVHTIPFDKRFRPSAARRVADLVRSERVDVVHAHGLVAATTCWMARRLFG
                     ARVPLLYHQHGFHHHNYGPLTIGLRRAAEKAICRRVDRVIPVSSADLALLSSGGYAPR
                     ERLELVYYGIPEPRATAEEIEGARRAARLEPGRPVVGLVGRLHVQKGVDVFLAAAALV
                     HEARPDAQIVVVGEGELETELHEQGRRLGLKDAVRWTGGRPSAPFMPLFSVAVLSSRW
                     EGLPLVLLEYMARSLPIVTTDAEGCLDAVGETEARIVPRDDPRAMADAILGLLGDPVS
                     AHRLGESARARFDGTFDIVTMTRRFESIYREVLR"
     gene            complement(14968..15960)
                     /locus_tag="PLP50_09645"
     CDS             complement(14968..15960)
                     /locus_tag="PLP50_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA51855.1"
                     /translation="MAAPVRPAPPDATAPRLSVQVVTWNSADVLDACLASLHAQTSPA
                     MEVIVVDNASTDGSAEVAERWFARGLPGTLRRESGNTGFCAAQNRALEISSGDWVLFL
                     NPDAELPADFVERALAVVDALPPDVGTVAPLILLPDGRVDSTGIVVDRFGRAYDRGRG
                     EPSADRYRSEEDVPACTGAAALHRREMLQDVAVGGLALDENLFAYYDDLDLSWRARLR
                     GWRCRYVPALVARHRRAGRNSLRAMADRPSRGSEQALTVRNRYLVMAKCERFRDLLGA
                     LPWLVPFELARPLFLAWNAPGALRGYALAARGLPEALRQRRVIQAGRGTRRGPR"
     gene            complement(15960..17165)
                     /locus_tag="PLP50_09650"
     CDS             complement(15960..17165)
                     /locus_tag="PLP50_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51856.1"
                     /translation="MRIAVLHPYPVSGRAVGGVTRLHALVRHLAPRHRVTVLAHAGED
                     PASDAEAVRELAEIGVKQLLFPRPGAGAVKRLSWVLGRSPYAVAYNRNPGLADALASL
                     EREEGLDAVHVELSYMAPFLARLQPRTVRILAEQETMSLALDRLRRVPFAGRTPFERY
                     LPLEKGKVRRFEAEHLRSFQLLYGISGTEASWLAARTGRRVGILPHVVATRTFVPAAG
                     EPTEPTVLFVGNFLHRPNLHGLTWFLESVWPGVRAAMPLARLEAVGPGLTEAWRREHE
                     RDGVLAPGRVENLVERYQQAAVFVNPIRTGGGMRGKVLEAFSCGRPVVSTPMGMEGID
                     AEPGRHHLEADEPQDFAGAVVRYLSDAGLRRAHGRAARELVDRLYDPSVVFTRLEDDA
                     LEAVSRSGR"
     gene            complement(17162..18592)
                     /locus_tag="PLP50_09655"
     CDS             complement(17162..18592)
                     /locus_tag="PLP50_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPA51857.1"
                     /translation="MRSLLANGISMLAARVLVPVFSFAINVGIARAYGAATLGVYIHL
                     LALLLIFQAAASAGMNLLLTRELATRPEESAAHLGSGRSFALLSGSVATLAFLAYSAF
                     LLPEDRLLSAAVLSLTILPSAWIATQEAFFMATRTHHRITVVSLAENALKLALAVAAY
                     LLGAGILGLCAGIAAARFLALGAGFVLMRRAGAPRTGRPTLAGALAFGRTVAPFAVLF
                     ALSMLYFRVDVLLVEALRGEGDTGTYGAALTLYSVVLLLPESAMGAVYPRLASSFRAS
                     AEGYAEATLLSLRLLVVGIVPLSLFLVCFADRVLGLAYGERFLSSATTLRLLAVSLPL
                     HAVNGVLGQALQAARLQGAMLRIVVLGLATHVVANVVLLSRLGIEGAAAALFLSSSVV
                     ALGAARCFHRNVAPIKPGARLFGSLATIGGPLVLALLAPPIWRPAAFAVSLLALSAGA
                     ISFLLRRSDLDRVVQAFRPPKLTVAS"
     gene            complement(18595..19590)
                     /locus_tag="PLP50_09660"
     CDS             complement(18595..19590)
                     /locus_tag="PLP50_09660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51858.1"
                     /translation="MTTIVLPDVSSSWAPLCPHPEWERIYGGLLSRCTACGLHATSEL
                     PEFVYDETYFTEDGTAGYNFESEFARAHDEARFEPELQRIEARGLKGSVLDIGCASGA
                     FLARAQARGWEVAGVEVADFARERTSALLGVPVASSLDGLPAGRRFDVVTLHHVLEHI
                     HEPLPFLRDEVAPRVGRLLVVEVPNFSSLASRVHGPRWRDLRPDQHVFHYTPSVLAKL
                     LSQAGFRPVRVTTLWEPLWSLRTALNCLRLLPALVRQPRHEAPPPGAGPAEVSDPASY
                     RRPTGPKRVLTEASRLAFRPLVRSLERAGLGERLVVEAEPLPGPSRSDPESVPKS"
     gene            complement(19587..21095)
                     /locus_tag="PLP50_09665"
     CDS             complement(19587..21095)
                     /locus_tag="PLP50_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPA51859.1"
                     /translation="MTTASESTAAAPSGRRVSWVLPGLLAGGLPFAVAAVAPSRTAFL
                     ILAASMGAAALAALVAFSPARMADGLFLGFVGVVSIPLAKHFFYQPHVGGWPGLRLSL
                     SDLFLSLAAPLLLAALLWGRRRNAVPAAVLLAYGLLLAWYSMSLVLSGSHRDLGLFEL
                     SSAVHALFLAFATAALFRRPLLTPFLVLLSCAVSVHTIFALLQAATGRPVGRSFFHGT
                     EVLRETLVSGAVRLRPSGLFDHPIVYANFLVLALPVLAAGYFVCSGGLKRALVAGTFS
                     LGTAGLILTLSRGAWFSAAIASISLLLLAWRRGLLAGAARRSVRRALAGAAGLVLALG
                     VLFGPAVYDRLARSQAGNVEVRYELNAIALRMIGSHPVGGVGLNSFIHVMERYDPRNV
                     KRYFPATVHNLYLLEAAETGLPGGLLFLLLIFTVVVSALRRLRTVADPALAWVAAALL
                     AGFLGFAVSQVADFSHRIEPLRSIVWVHLGLLFGILGVGSAGTTPDQEVVPA"
     gene            complement(21128..22351)
                     /locus_tag="PLP50_09670"
     CDS             complement(21128..22351)
                     /locus_tag="PLP50_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA51860.1"
                     /translation="MRLFVSWGAALLFLALGPGSGLPASGVPWGPRSDEVARGQRVLM
                     AFAHEARASLAESRGGSLHGGSMRADVRASLLAAFASAEKALERSLATSPQPSGSTTL
                     HDGALVGRLRIATVDQVALDVVAYASGGLVVAGLLAYPDDGTPHSTVLHVHGGFGGIF
                     TNADGDMVQTCIDWAGRYGRTAFAPSLRGQDGGEGAPELCLGEADDVGAAAALVRSLE
                     VTDPDRVALVGGSTGGCVVLRAAPQIEGLRAVVAYVPPTDWKGLVAFHRTSWAPATES
                     LCEGGSRLWDVGGPALADVLDQMICGHVDCTDAEYEVRSPIPGVPFQTAPTLVVSAGS
                     DNVVPLDQQVLYSVLRQGSGHPVDVYVTDPCDPAGTAAGATDTLLHVREAYHLLSVGA
                     VGSGFLYLMQELDRP"
     gene            22562..>23193
                     /locus_tag="PLP50_09675"
     CDS             22562..>23193
                     /locus_tag="PLP50_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPA51861.1"
                     /translation="MTSKSAPSARGTKYVYSFGGGSAEGDGSQKNLLGGKGANLAEMA
                     RIGLPVPPGFTITTEVCTHFYANGRSYPKGLEKEVAQHLAGVEKKLGKKFGDPKNPLL
                     VSVRSGARASMPGMMDTILNLGLNDVTVEGLAKASGNPRFAWDCYRRFVAMYGDVVLD
                     LKPVGKHERDPFEVILEAKKEKFGAHLDTELTVEALKELVGEFKKAIKEKT"
BASE COUNT         3621 a         7794 c         8675 g         3003 t
ORIGIN      
        1 cgcggagctc gttcgcgagg aaccgcctga gggaatcgag tacgttcatg tcgtgcctcc
       61 cgggcgactg cccgactggg tccggctcgc cggcgccccg ctgctcgcgg gttcaggccg
      121 gtcggcgagg gtcttgaggg cctggcggtc gatctttccg ctcgaggtcg tggggaacgc
      181 cgccaggaag cggatctccg ccgggaccat gtacttgagg acgtgggacg cgaggtggcg
      241 cctgaggtcc ctttcgcggc tcgggtccgg tccttcgccc gagacgaagg cgacgagctc
      301 cgtctcgcgg ccgctcgacc cttcccgctc gaccgcgacg accgccgcct cggtcacccc
      361 cgcggcgcgc aggagggccg attcgacctc gccgagctcc acccggtagc cgcgcacctt
      421 caccatgtgg tcgacgcgac cgtggaaggc gtaggtcccg tcgggctgcc ggcggacgat
      481 gtcgccggtg cggtagaggc ggtcggagag gtggggatgg aggaagtcgg cgacgaacga
      541 cctcgccgtc gcctcggcgt ctccccagta cccttccatc aacgtcccgc cgcggacgag
      601 gagctccccg gtctcccctt cgggcaccgg cctcagctcc gggtcgacga cgacgccctc
      661 gcagttctcg cagtcgatgc cgatcgggac cgccggtgac gtccaggtgc ccgccggggg
      721 gacccgccag taggtgcaga cgttcgtctc cgtcggcccg tagaggttgt agagggcggt
      781 gtccggcggg agggcgagct gcagcgcccg cagctcgtcg gcggggtaga cctcgccggc
      841 gaagaggacc gtccgcaggg cggggagctt ccggtcggag aggcgggccg tccggaccat
      901 ccgccggagg gcccccggga ccgagtacca gacggaaatc cgttcggtct cgaggacctc
      961 cgcggaggcc atcgggaagc gaacggtctc ctcgtcgagg acgacgaggc gcgctcccgc
     1021 gagagcggcc ccgaagaggt cgaaggtgga gaggtcgaag tggagaggcg catggctcga
     1081 gaggacgtcc tcgggacgga gctcgaaccg ggcggcggcc cattcgacga acgacaaggc
     1141 cgcgccgtgc gacaccatca cgcccttcgg cacgccggtc gaccccgacg tgtagaggat
     1201 gtaggcgagg tcacggacgt tgggagaggg gagcggtccc gccggaccgg gtgggactcc
     1261 tgccccgccg tcgccgtgca ggagaaggac ggggattccc ggcagcgccg ctcggatcgc
     1321 ctcgaccgcg tcgggaactt cggtcgaggc cagcagcgcc gacattccgc agttcgacgc
     1381 aatgagcgcc gcgcgctgag gcggggagga ccgatcgatc gggacgtacg cggcccccgt
     1441 gcggagggcc ccccacacgc cgacgacggc ctcgaccgat ttcgggagga ggaggccgac
     1501 gcggtcccca cggcggacgc cgcgctcggc gagccaggcc gcgacggacg cgctcgcccg
     1561 gtcgagcccg gaataggtca gaggctcctc tccgggaagc ccgacggctg cccgaccggg
     1621 gttttccctg gcggaccgga ggaggaggga gacgagcgag gccggcacgc tcagagcccc
     1681 aggtgacggg cgacgatcat cctctggatc gcggaggtcc ccgagtagat cgtcccgccg
     1741 acggcgtcgc gaaggtccct ttcgatctcg tactcggcca tgtagccgta ggccccgaag
     1801 agctcgatgg cgtcgaggct cgactggacg aagcactcgc tgacgtagag cttcgccatc
     1861 gccgactcca tgctcgcccg tttcccctgg cccttcagcc acgccgtctt gtagaggagg
     1921 agccgggccg tctcgagccg caccttcatg tcggcgatct tgttcgacac cgactggaac
     1981 ttcccgatcg ccttgccgaa ccgctgccag gtacgggccc tctcgacgca cgtctcgagc
     2041 tggcgctcga gcgtcccgat gttcgccgcc aggatgcagg cccgctcgta gtccatcgag
     2101 ctgttgaaga gggccacccc ggctccctcg ccgccgaggc ggcgctccgc cgggacctcg
     2161 cagtcctcga ggaagatctg tcccatcggg gaggtcctga gccccatctt ctcgaagggc
     2221 cggctgaccg agaagcccgg cgttcccttc tccaccagga acgcggagat gccgtccggt
     2281 ccgctcgaga ggtcctcgga cgcgaagacg acgacgacgt ccgccactgg ggcattcgtg
     2341 atgaagagct tcgagccgtt gaggacgaag cggtcgccgc gccgcgtggc ggtcgtccgt
     2401 acgttgaagg cgtcggaccc cgagccctcc tcggtcatcg cgtggacggc gatccactcg
     2461 ccgccgacga gcttcgggag gtaccggtcc ttctgctccg gggtgccgaa cttcaggatc
     2521 ggcatctcgg cgctccagac gtgggcgttc agcgagaaga tcagcccgtt gtcggggcag
     2581 ccgtagccca agccctccat cgcgcagacg gtcgtcagta ggtcgcggtc ggttcccccg
     2641 aacgcctccg gcatcgggag gccctggagc ccgaagcgcg cggcggcctc ccacttcgtc
     2701 cgggagaagt ggccggcctt ctcctcttcg aggaggtccg cgccgagctc ggccctcgcg
     2761 aaggccgtca cggcctcccg gagctctacc tggtcgctcg tgaagtcgaa gtccatgctc
     2821 ttctcgaatc ggtcagtgga aggtccagaa gtgctctgcc gaatagctca ggttgcgcgg
     2881 gcgcttcgcc acgacctggg ccgggttgcc acgcaggatc gagtaggggg gcgagctctt
     2941 cgtgacgacc gacccggcgg tcacgacggt cccttccccg agcgtcacgc cgaggaggat
     3001 ggtggacccc gtcccgacga agacgtggtc ctccatcgtg atcggccccg tgatgccgtc
     3061 gaacgccggc gagaagacgt cgtggctccc catgacgagg ttcacccggt aggcgatcgc
     3121 gacgtcgtcg ccgatcgtca ggtcgttcat ccccgccccg acgatgaggc agtccatctg
     3181 gaaggtgctc cggttgccga tcgtcacccg accccggccc ccgaagatct tgagtccgtg
     3241 gtggacgctg ctccgctcgc cgatcctcca gccgagcgcg ttccggtagt acgcgaggcg
     3301 cagcgcgtgc cccggtaggg gcccgacgac gcggttcgcc ccgaagagga gggcgttgcg
     3361 cgcgagctgc ttgaggcggt cccccaggga gtcctgcacc cggacccggc cggcgtgccc
     3421 gagggacgac gcgagggcgt cgggaagaag aggcgccttg ccgctcacgg ctcgcgcacc
     3481 gccgctgcga ggagcggcgc cagtcccgtc accctcacct cctgggccac gcaggccgcg
     3541 aagccgagga ggaggccgcc ggcgaccgac gccaggagga cgatcagctt cttggggcgg
     3601 accggcttgg cggacgcggc cgcctcctgt acgacggaga cgttcgtgaa cttccccgag
     3661 tcgagcgccg agtcgatgag cgctcgctcc tgctgcgaaa ggaccatcat gtagcgatcc
     3721 tcgaccgagc ggatgaggcg ctgcgcccgc gaggcggcga cgcttccctc gtcgagctcg
     3781 acgaggcggg cccgggaggt ccgcagctgc cgctccagcg cctgtcgccg ctcgacgatc
     3841 ccggcccgct cggccacggc gcgctcccgc tcctcgcgga gcgccacggc ggtcggcccg
     3901 tgctgcgccg cgtactgccg ttcctcctcg agggccttcc gggcggcttc gagcttcctc
     3961 cggttctcga ggacgggcgg ggcgtcgtcc tggtaccggc cctcgacccg gatctgctcg
     4021 agctgcaggc ggagggcctg gaccttgaac tcgtccagcg cgggctggct cgccgcggat
     4081 gcggcggagt ccgtctcccc acgcgccgcg cgctcgagga gcctgtcgta cgcggcgatc
     4141 ttcccgtcga gctcgacgag gcgccggccg agcatctcgt tctcctcgcg aaggcccgcg
     4201 tcgagcttca gctggacctc ccgctcggtg ggcatgtcga ccgcgcgcac cctcttcttg
     4261 atcgcctgga gggcgtcctc gtgccgggcc agctcccttg cgagggtctc cgtctgagag
     4321 gagaagaagg ggacgacccg ttgagtgccg tggacctcca cgtggtgggc gacgtagttc
     4381 cggaggagct ccgccaggac cgcctctgcc acaccggggt cccccgcctc gtaggtgacg
     4441 acgaggatgc gggactcctt gagccgctcg acgccgagcg agccggagag cgcccggatc
     4501 gcgcggtcca cgtcgttcgg aggtgtgacc tcgtggtacg cggcgtgggc ccgctcgtag
     4561 gcccgaagcg gcgcgaagag gagccgccag aaccaggaag gccgggcctt cgcgaggccc
     4621 gtcgcctcga ccgtagccga gaggactcgg cggctcgtca gcacggcgat ctcggaattg
     4681 acctcctcct cggtgatctc ggcgacgagg acgggagact cggcccgggc cggggaatcg
     4741 agccgggggg tgtaccggct cttctggatc atcacggccg cccgcgcctc gtaggtcgcg
     4801 ggccagacga aggcgaacac ctggatggcg aggaaggtcc cgagagcgac ggccgcgaag
     4861 agacgccgcc gggacgtcac cgacctcgcg aggcgcagca atgcctccgc gaggtcgccg
     4921 ggctgcgccg cggcaggtgg cgccggtgtg gcgctcccga cggggtggat cgagtcgctc
     4981 aagcggcctc ccgagcgccg gcggcgtcga gggcaccgag cgtacgctcg agctcgtcgt
     5041 cgagctcgga gacgaacggg gcccagccga agcgggctgc gctcgaaggg gcgttcatcg
     5101 cgaggcgctc gtggagaccg ttctcgccgg cgagccgcgc catcgccgac gcgaacgcta
     5161 ccgggtcggc ctcggagagg agcccgtcgt gcccgtgccg gacgatctcc ttcgggccgc
     5221 cccggttcac cgcgaccgcc ggcttgcccg aggccatggc ctcgatgatg acgagccccc
     5281 agtcctcgtt gaacgcggtg aagagcgtcg cgaagcaggc ggcgtacagg gcacgcatct
     5341 cttcgtccga cgggtggatg cggaactcca ccgaggggtc cccgccagcg agggaccgga
     5401 gacgttcgag gtacggccgg ctcttcctgt cgacgatccc ggcgatgacg agcttccagg
     5461 tcgagggctc cggcgccgtc tccttgaaga gctggaacgc ctggatcgcg agctcgaggt
     5521 tcttcgtcca catgatccgc cccgggacga agaagaccct ctcggccgga ggcgacggcg
     5581 tgaaggcgga aagctccacg ccggggtgga gcgtccgcat ccgctcgggg ggcgccaggc
     5641 gccccgccag gatgcgcgtc cgcacctcct cgctgatcgc gaagacccga cggtagcgcc
     5701 gccaggcgat ccggtcgacg ccgcggtaga ggaaggtccc ggctcggacg acgacgcggt
     5761 gaagggcatt tcgggacgcg aggtagttcg cccgatacac ggggtcgaag gcgatacgga
     5821 ggggagtcag gcagaggcag aaaacgggga tccggcgctg acgaagggcg acgaggtccc
     5881 cgagcccctc gcagacgacg aggagaccgt cgaactccga gaggtcgagc tcctgggaga
     5941 ggagccgcgt cgcggcgctc gccaccgacc ggagcgacct ctcgacggga acgcgggaga
     6001 ggaggcggac gtccatctgc cccagctcgg ggaaggtcgc gtcgggctgg tactcgttcg
     6061 tgaagatcgt gatccggtat cggctccgct tcgcgatctc caggatcgtc cgctcggcgc
     6121 cgctcgtcag gtagagccac gggtagtaca ccgcgagccg cctgcgcctc ggcgcgtcat
     6181 cgttctgtag ggtcatacgg aaccccccac agtaccatag cacccggcgg ccgggcggag
     6241 gctcaccgcg cgaaacggaa gatcgtgagc gagtgcgggg ggaacctgta ggagaaggca
     6301 cccgcggcgc cgaagggcgt cgtggtgagc gccacggcgt ccggtctctc ggcgctgttc
     6361 cgagcgccgg gtccgggacc gttcaggacc gagacggtgc cggtcacgcc ggacgggagg
     6421 ttcatctcga tcgttgcgtc cacggccgtc gagaggctcc ggttcacgac gaacagagcg
     6481 aagtggctct cgccgggatc ccgggtggcc agggcgtcga gcatcgggac gtccatctgc
     6541 cccgagagga gcttcaccgg cttcgagtcg aacagcgcgt ccttcgggtc gagccggact
     6601 tcggtcatca ggttcccgga ctcgcgcgcg tactcgcggt gcacgtagaa ctgcgggttc
     6661 cggaaccggg cggctccggc ggtcccgatg aggctgccga agtgagtccc ggcgagcgac
     6721 atgtggtgcg ccccgtacac gcgctcgctt cgcgccatga cgtggaggac gctcgcattg
     6781 aagagggcgc tggcgagcgt cgggttcctc tggtgggtcc ggacggggtc ctcgatggcc
     6841 tcgtccgggt agaagatccc gtcgtactcg gtgacgaaga tcgggatgag cttcccgagc
     6901 cttcggagct gcccttcgag cacggtcatg gacctctcga cggccttcgt cgctcccagc
     6961 atcgacgtga agagctcgtc gtccggaggg atcgtcccat cggccctgta gccgtagagg
     7021 gggaagtagg cattgtggac gctgatgccg tccacgtagg gtcccgcgag cccgaggacg
     7081 acgctgttcc agaagctgtc ctgctcctcg gggccgatga ccgccgccga gtagatcttc
     7141 gcgtacgggg cctgcttgcg gatggcggcc gcgtattcga tgacgcggcg ggcgtagacg
     7201 tcgggcggga gaccgatctg gccgctctcc gacaggccct cgaaatagac ctcgtttccg
     7261 acctcccagt agacgacggg gaacccgcgc gactcgaaga accgcgcgaa ggcggccgcc
     7321 tcctcggcgc tgccggtgcc ggtgttcgcg gtgacgaagg ggaccgcctt cagcgccgtg
     7381 gcgagcttga tgaactcgtc gggccccacg accggggagt agaactcccc gaggtagccg
     7441 ctcgagagct tcgggcgccg cgagtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnccgg ggagtagaac tccccgaggt agccgctcga gagcttcggg cgccgcgagt
     7621 gcgggccgag cgcttgccac cagtggaaga agtccgcggc cgtccccccc gggaagcgga
     7681 gcgtcgtgac ccgggcctcg gagaggaggc tcacgatctc gcggtcgaag gagccatccg
     7741 gcttgacgat cccttcgccc cgctcgatcg tctggaggtt ctgcccgaag agaagagccg
     7801 ggatcacccg cttcaccttc tggaagtcga cgtggatcga gctcgtggcg acgggcatcg
     7861 gctgcgcgcg cgcaggccgc gcggcggcga agaccacgag gagcgccgcg gcgttcgctg
     7921 cgcgggcgat ccgcgaaatc catgacctgt gggagagggg gggagtcttt ctcattcgtc
     7981 tcattcgaac gggaagtacg ccgttcgtcc gtccctctgg aacacgagac gcgtaacgag
     8041 atcggcagga gtcgcgttcc agagggacca ctcggaacgc cagctgtcgc cgtcgagggc
     8101 gagcgggaag acaggggcga ggtccctcgt accggcggct ccgtcgagga cggggaagcc
     8161 cgccaggatc tcctcgcccc ggtcgttcac gcggcggagg aagccgacga cgtcgagcgt
     8221 cgcaccgttc gggccggcga ccgtgccggc cagggacccc tgaaggtcgc ccgccgaagg
     8281 gaagagggag cggcacgata tggccacctg ccctccggcc gggaccggga cgtctgcgga
     8341 cccgaccgtc cctccgcccg agtcccgcag cgtcagggtc gccgtgacgg ccagtcccag
     8401 gcggttcgtc agtatgaccc cgctgtccct gagggcgagc tccaccggaa cgaggaagcg
     8461 cgtcaccgcc cgggagggtg gaacggtgcg gggcaggacg acgggttgcc cggaggtgtt
     8521 tcccacggtc ctctcctcga cgagctggac catcggggcc ggctggctgg cgctggggcg
     8581 cggagcgaga gctccgaccg cctggtcgtt gcgcggcagg acccggacct gggccgtccg
     8641 aacctcggcg tcggaagggc gcgcgtccgg tacgcggaac cggaagaccg cggttccccc
     8701 gggcggcagc tcgtagcctg cggtcgtggc gtggccggac tcgagctcga cccggagcgg
     8761 agcgccggtg tcgtcccgga acgcgagggt ccccgaaagg cccgccaggc cggtgttcga
     8821 gactgcgagg cggtggaccg tgtcgggggt cagaagaatg ctctgtactg cggcgctcga
     8881 cgggaccccg ctgtcggcgt cgttgaaggg ggcgacgagg acggggtcga gaaagctcga
     8941 cggcctgagg ttcgccctca tggagtggac ggacgtggcc agcgggcggt tcgagacgag
     9001 cgtcaggagt ccgcggacct gctccgggaa tgcgaggagg tcggacagga actggaccgt
     9061 gaagcccccc gccggcacgc ggaacgcggc ggacgtcgtc tggatgggcg tgccgtcgag
     9121 cccgcgcagg acgaggtcca cggaggcctc cgccgtgccg gggttatacg ccgtgatccg
     9181 ggtccggccc gccgttcccc cttcctcgac gtaggcgtgg ctcaggagcc gcggctccga
     9241 ggagacggtc gccagctccg tcacgacggt tccgtcgagg cggccgctca ggacctgaag
     9301 cgcctccggc gccggagcgc ccgtggcggg cgtgaggaga agcgccgcga gcctcggggc
     9361 gtcgtcctgg cccgtggtct tcaggaaggc ggagcccgac gccggcacgg cgagcgtcgt
     9421 gatcgtcggg atgtaccgcg ccgtgaggtc ggtgacgaag gtgtcgcggt gcaccaccac
     9481 gctgtgctcc cggtcgccga ggtcgctcca gccatcgaag gcgagcggcc gggagccttc
     9541 tccccgatcg acgagctcgg gggccgagac gacgtggacg gagcccgacg cccattggaa
     9601 cgttgcggga gtcgtggacg aggccccgtc gacgccgagg gcgaggccgg agggctcgct
     9661 cttcacgacg agatggagcg gctcgaagtg tgccttcacc gagcgcgggc ggtccatgac
     9721 cgcgaagccg aggatgccgt cggacccgct cacgtcgccg gagtagcccg cgaaccggtg
     9781 cacgggagag gggatcgcga cgagcgtcgc gaagctcctc tcgacgtaga agccgtccgc
     9841 cgagggcggg tcgagagcga gcgagccgcc cgaggtcggg gtgacctcgg tgcggaggcg
     9901 gtgcgatcgg cggatcgcag ccgtgaacca ctcgcgtccg gtcgtcgtcg tgaccgggtg
     9961 gctcgatgct ccgccgtcgc tccaggagac gaagacctgg cgggtgtcct cgttctcgaa
    10021 aaggacgggc tcgatccgat ggacggaccc gcgggcccag cgagcgtagt agggagtcgt
    10081 gacgacctgt ccgtcgacga ggatctcgag tccggagggg atcgtgtcga tcgtcacgag
    10141 ctcctccagc ggaatccgga agacgccgcg cccgaaagtc ccggcgaaga gggtgtcgtc
    10201 cgccacccgg agcgcgctca cccccgcgcc ggcgagcccg ttgcggaacg ccgaccagct
    10261 ggatccgtcg ttgggagagc gccagactcc gtcagacgtt ccgatgtaga gcgctccgaa
    10321 cgcgtccttg acgatgctgc tcggcgtcgc gaggcccgga aggctgatct cctcccacgc
    10381 ttggccgaag tcgtaggact tgaagacccg ccccgtctgg gacgaagggt cggcgagcgt
    10441 cgcgtacagg acgttctccg cgtccggatc gaggtggagc gacgtgacgg cgagtccggg
    10501 gtcgtcgctg atcgtgatca ggtccgtgcc gatgccgtcg gccttgaaga gcttcgctcc
    10561 gagggcgacg gcgaagtaga cccggccgga gcggaccggc gacaccgcga tccgggtgat
    10621 ctccggcgac cccggggggc ggaagccggt ccaggagtcg ccggaatcgg tcgaccggaa
    10681 gagctgcatt cggcccgtga agagagtccc gggatcgagt gggctgggct cgaggggggc
    10741 ctcggtcggg gcgggctgat cgaggtcgag cccctggacg atcgggcgga agctcactcc
    10801 gcggtcgtcg gagcggaaga ggcgcccccc ggtcgaggag acgtagaggc ggtcggcgtc
    10861 gaaggggtcg aagcgcgccg ggcccgccgc ttcccgcctc ccgtggatga cgtgccagac
    10921 cgggacgggg tcggccggca ccgcgagaag ggtgcccgag tcggccgttc ccgcgaggag
    10981 gcgagggggc gactcgcggg aggcgtccag gctcgtgatc gaggtgacgg cgagcccgta
    11041 gctcgaggac gtcgccgtcg accaggccgc gtcgagcgtt gcgactcccg aggtccccgc
    11101 cacgaggacc cgatccgcga ggaccaggat ccggtgcacg ccggccgcgt ccccggtgac
    11161 ggccctccag gtcgctccgg cgtccgtcga aacggaaagc ccgtccccgc cgaggaggat
    11221 ccgcgcgtcg tcgagcgggt cgagcgcgat cgcctgggcg aatccgcagg aggtgcagaa
    11281 cggaggaggc gcgagctcct cgaacgtcgt tcctccgtcg cgagagcgca gcaggagagc
    11341 ccggtcggtc ctgttgaggt cacccagcac ctggatcgtc gcgtagagaa gtcccggagt
    11401 cctcgcggac accgccacgc tcgtcgtccc gaccccgaac gtgtcggtgg cccggagcgg
    11461 ccacccggcc gttctcaccc acgtgctccc gcggtcgtcg ctccggtaga ggcctccccg
    11521 gcccgacgcg aagaccgaac cggcgcggag agggtcacgg accagcatcg acacggcatc
    11581 cccatcgagg gtcgcggcga aggtcgtccc cgcgtccgtc gagagcttca cgccggtgtc
    11641 ggtggcgacg aggacccgcg atggatccgc gtcgtcgacg aggagtgcgg agacgtagag
    11701 acccgcgccg atgggtccgg cgacggtcca tgaggtgccg ccatccgtgg accggatcag
    11761 cccgatcccg ggggagaccg agcccccgtc cgagagtgcc ccggtcccgg caaggaggac
    11821 gttcgagtcg acgggggaaa tcgcgacggc gctcaggggc gtcgccgcca gccacggcgc
    11881 gagcgtctgc cagacctgcc cgccgtcggt ggagcgccag aggccacccg ccggggtccc
    11941 gacgacgaga tgccgggggt cgcgcgggtc cgccgccagc gcggagaccc tgccgccgac
    12001 cgagctcggt ccgagctcct cccagcccgg ctcctgtgcc cgggccgccg ggaaaaccac
    12061 gaacaggccg gccagcgcaa gccggcgcca ggggagcgtt tggagcattt ccgagatgtt
    12121 atctcatctc gtgaggccgg cccgccgggc cgggccccgt cgcggcgctg tcccgccccg
    12181 gcccgttcct acttgcgggg aggagcgacc gaggtcgtgc gagccggcgc ctgctcggcg
    12241 acgaggctca ggatcgcgtt tggcgggatc tcgacctcga gggggtttcc agccgggcat
    12301 ggccatggcc tcggtgcgat ctcctcgccg ccggtccggg tcgtcgtcgt gagccccggc
    12361 ccgttgatcg aggccggaga aggggccgtc aggaggaggc agtcggtcga tcgcggcgcg
    12421 aagcccgcca tggaaacggc cgtgcggagc ggccgatcga ggctccgatt cacgaggagg
    12481 gccgtcaggc ggctgccgtc ggcgctcagg ctcgcgcgcg cgaggagatc gggcagcccg
    12541 ctgtgagcct tcacgatccc gttggcacgg gcgtcgaagg tcggagaggt gacgaccgtc
    12601 gagaggagac ccggctcgaa gcgggagcgg tagagcgagt agaggtgata ggtcggcgtc
    12661 cggacgagcc ccgcctcggt gtcggtgagg aggctcccgt accagcgatg gatcgggttc
    12721 gtgtagcagg cgaggaagat ccgaggctcc ccgaggaaga cgtcgaggag agacgcaacg
    12781 tagagggcgg ccgccagcgt ccgcgactgg tcgacgtaga tggcgtgctg gtcccgccgg
    12841 gagctcacgc cgaagagagg tccgaactcc gtcacggcga gaggcacgtt cgcgttcgcg
    12901 ggggagaggc gggcgagcgt ctccaggacc tggtccaggt tctcccgggt ctccgccgcg
    12961 cccgcgtaga ggctccggta gacctcgccc ctcgcgcgct cgtcggcgaa ggaggccgag
    13021 tcgtcgagga tcacgggcgc gtaggcgttg tggacggcca ggaagtcgac gggtccctcg
    13081 agggactcca gcatccggac ggtccagtcg cgactttcct cgggggccag ggggaacgcg
    13141 cccgtgtcga ggtgtccgat ggccccgacg agcgcgtccg gaaagaccgc tcgaagagcg
    13201 gaccggtagc gcgggaagtc cttcgcgtac tcctccggcc gacggtagat ggccttgccg
    13261 ttcggtccgc cttcgcgcgg cccggtcaga tagatctcgt tgccgacctc ccagagccgc
    13321 gcccgtgcac cgagagacct gaggtgcttc gcccaggccg atgcctgctc ggcgctgccg
    13381 gtcccgtagt tcgccgtcag caccgcctcg gcgccggtct tctcgagcag cgcgacgagc
    13441 tcgggcgtgc cgaagcggtg ctccaggctc tgcccgttga aggcgttccg gctcgagccc
    13501 cgatggttcg ggccggcaat tccgagcgac cagtcgtagt agtcggcgtg gatcccgccc
    13561 gggaaacgga aggccggcac gcgaagcggc tggaggagct cgaggacctc ggagcggagc
    13621 gcgggccgat cggcctcgag gagcccgttt ccgttctcga cccactcgat gtgcataccg
    13681 aacaggagcg ggctcacgag cctgaggacg cgcgtggcgt cgatcgtgac tttggcacgc
    13741 tccggtgcgg ccggaggtgt ctggggcgcc gccgccacca cgccggatcc ggacgccgag
    13801 gcccggttct tcccgcagcc cgcgacgagc acggtcagca tcggaaggag aaggaccgcg
    13861 cgtttcacct caggacctcc cggtagatcg actcgaaccg gcgcgtcatg gtcacgatat
    13921 cgaacgttcc gtcgaagcgg gcccgggcgg attctccgag gcggtgcgcc gacaccggat
    13981 cgccgagcag cccgaggatg gcgtcggcca tggcccgggg gtcgtcccgg ggaacgatcc
    14041 gggcttccgt ctccccgacc gcgtcgaggc agccttccgc gtccgtcgtg acgatcggga
    14101 gggagcgtgc catgtactcc agaaggacga gcgggagccc ctcccagcgc gaggagagga
    14161 cggcgacgga gaagagcggc atgaaagggg cgctcgggcg tccgcccgtc cagcggaccg
    14221 cgtccttcag gccgagccgt cgcccctgct cgtggagctc ggtctcgagc tccccctcgc
    14281 cgacgacgac gatctgtgcg tcgggtcgcg cctcatggac gagggctgcc gccgccagga
    14341 agacgtcgac ccccttctgg acgtgcagcc ttccgacgag ccccacgacg gggcggccgg
    14401 gctcgaggcg ggccgcccgc cgggcgccct cgatctcctc cgccgtggcg cgcggctccg
    14461 gtatcccgta gtagacgagc tccagcctct cccgcggcgc gtagcccccg ctcgagagga
    14521 gcgccaggtc cgcgctcgag accgggatga cccgatccac cctccggcag atcgccttct
    14581 ccgcggcccg gcggaggccg atcgtgaggg ggccgtagtt gtggtggtga aagccgtgct
    14641 ggtgatagag gagcggcaca cgggccccga agagccgcct ggccatccag caggtcgtgg
    14701 ccgcgacgag gccgtgggcg tgaacgacgt cgaccctctc cgaccggacg aggtccgcga
    14761 cgcgccgcgc cgccgagggg cggaaccgct tgtcgaatgg gatcgtgtgg actctcgtcc
    14821 cgagcgcccg ggcggcctcc acgagcgggc ctccgtcgcg gcaggccagg agcgactcga
    14881 cgtcggtccc ccggaacccc tcgaggaggt gccggacgac ggtctgaccg cctccgagga
    14941 aggacttgtc gatcacctgg aggactctca acgcgggccc ctcctcgttc ctcggccggc
    15001 ctggatgacc cgtcgctggc gcagcgcctc gggaagcccc cgcgcggcga gcgcgtatcc
    15061 gcggagggcg ccgggcgcgt tccaggcgag gaacagcggc cgcgcgagct cgaacgggac
    15121 gagccagggg agcgcgccga gcaagtcgcg gaaccgttcg cacttggcca tgacgaggta
    15181 gcgattccgg accgtcaggg cctgctcgct gccgcgcgag ggccggtcgg ccatcgcgcg
    15241 gagggagttc cggccggccc ggcgatgacg cgcgaccaga gccgggacgt accggcacct
    15301 ccagccccgc aggcgcgccc gccacgagag gtccaggtcg tcgtagtagg cgaagaggtt
    15361 ctcgtcgagc gcgagcccgc cgacggccac gtcctggagc atctcgcgcc ggtggagcgc
    15421 cgcggcgccg gtgcaggccg ggacgtcctc ctcgctccgg tagcggtccg ccgaaggctc
    15481 acccctcccg cggtcgtacg cgcggccgaa gcggtcgacg acgatccccg tgctgtcgac
    15541 ccgtccgtcg ggcagcagga tcagcggcgc cacggtcccg acgtccggag ggagggcgtc
    15601 gacgacggcg agcgctctct cgacgaagtc cgccgggagc tccgcgtccg ggttcaggaa
    15661 cagcacccag tcgcccgagg agatctcgag ggcccggttc tgcgcggcgc agaagcccgt
    15721 gttcccgctc tcgcgccgga gcgttcccgg gagcccccgc gcgaaccacc tctcggcgac
    15781 ctccgcggag ccgtcggtcg aagcgttgtc gacgacgatc acctccatcg ccggggacgt
    15841 ctgggcgtgg aggctcgcga ggcaggcgtc gaggacgtcc gcgctgttcc aggtcacgac
    15901 ctggaccgag aggcgcggcg ccgtcgcatc cggcggcgcc ggccggacgg gggccgccat
    15961 cagcgtccgc tcctcgagac ggcctcgaga gcgtcgtcct cgaggcgcgt gaagacgacg
    16021 gacgggtcgt agagccggtc cacgagctcc cgggccgccc ggccgtgggc gcggcggagg
    16081 ccggcgtcgg acaggtaccg gacgaccgcg cccgcgaagt cctgcggctc gtccgcctcg
    16141 aggtgatgcc ggcccggctc cgcgtcgatc ccttccatcc ccatcggtgt cgagacgacg
    16201 ggccgcccgc aggagaaggc ctcgaggacc ttccccctca tcccgccccc ggtccggatc
    16261 gggttgacga agacggccgc ctgctggtag cgctccacga ggttctccac gcgccccggg
    16321 gcgaggacgc cgtctcgctc gtgctcccgt cgccaggcct cggtcagccc cggtccgacc
    16381 gcctccaggc gagcgagagg catggccgcc cgcaccccgg gccagacgct ctcgaggaac
    16441 caggtgaggc cgtgaaggtt cgggcgatgg aggaagttcc cgacgaagag gacggtcggc
    16501 tcggtcggct cgccggccgc ggggacgaag gtccgcgttg cgacgacgtg cggcaggatc
    16561 ccgacgcggc gtccggtcct cgcggccagc catgaggcct ccgtgccgct gatgccgtag
    16621 aggagctgga aggagcggag gtgctcggcc tcgaagcggc gcaccttccc cttctccagc
    16681 gggaggtagc gctcgaaggg ggtccggccc gcgaacggga ccctccggag ccggtcgagc
    16741 gcgagcgaca tcgtctcctg ctcggccagt atccgaacgg tccggggctg gagcctcgcg
    16801 aggaagggcg ccatgtacga gagctcgacg tgcacggcgt ccagcccttc ctcccgctcc
    16861 agggaggcca gggcgtcggc gaggcccggg ttcctgttgt acgcgacggc gtaaggggac
    16921 cggccgagga cccacgagag gcgtttcacc gctcccgcgc cgggccgagg aaagagcagc
    16981 tgcttcacgc cgatctccgc gagctcgcga acggcctcgg cgtccgaggc cgggtcctcc
    17041 ccggcgtggg cgaggacggt gacccggtgg cggggagcga ggtggcggac gagggcgtgg
    17101 aggcgggtca ccccgccgac cgcgcgcccg ctcacggggt aggggtgcag gacggcaatc
    17161 ctcacgaggc gaccgtcagc ttcggcgggc gaaaggcctg gacgacccgg tcgaggtcgg
    17221 accggcgcag gaggaaggag atggccccgg ccgagagagc gagaagggag accgcgaacg
    17281 cggcgggtcg ccagatcggg ggagcgagaa gggcgaggac gagcggaccg ccgatcgtcg
    17341 cgagcgaccc gaagagccgc gccccgggct tgatcggcgc cacgttccga tggaagcagc
    17401 gcgcggcccc gagcgcgacg accgaggacg acaggaagag ggcggcggca gctccctcga
    17461 tcccgaggcg cgaaaggagg acgacgttgg cgaccacgtg cgtggccagg ccgaggacga
    17521 cgatccggag catcgcgccc tggagccggg ccgcctgcag ggcctgcccg agcacgccgt
    17581 tcaccgcgtg gagcggcagg gagaccgcga ggaggcggag cgtcgtggcc gaggagagga
    17641 accgctctcc gtatgcgagg cccaggacgc ggtcggcgaa gcagacgagg aagagcgaaa
    17701 gggggacgat cccgacgacg aggagccgca acgagaggag cgtcgcctcg gcatagccct
    17761 cggccgaggc ccggaaggac gaggcgagac gcgggtagac ggcgcccatc gcgctctcgg
    17821 gaaggagcag gacgaccgag tagagggtga gggccgcgcc gtacgtcccc gtgtctccct
    17881 ccccgcgcag cgcctccacg aggagcacgt ccacccggaa gtagagcatc gagagcgcga
    17941 agaggaccgc gaacggggcc accgtgcggc cgaaggcgag cgcgcccgcg agcgtcgggc
    18001 gccccgtcct gggcgctcct gcccgacgca tcaggacgaa ccccgcgccg agggccagga
    18061 accgcgccgc cgcgattccg gcgcagagcc cgaggatgcc cgccccgagc aggtacgccg
    18121 cgacggccag cgccagcttc agcgcgttct cggcgaggga gacgacggtg atccggtggt
    18181 gggtccgggt cgccatgaag aacgcttcct gcgtcgcgat ccaggccgag gggaggatcg
    18241 tgagcgaaag gacggcggcg gagaggagcc ggtcctcggg cagcaggaag gccgagtagg
    18301 cgaggaaggc gagcgtggcg acggagccgc tcaggagagc gaaggacctc ccgctcccca
    18361 ggtgcgccgc ggactcctcg ggcctcgtcg cgagctcccg ggtgaggaga aggttcatcc
    18421 ccgcgctggc ggccgcctgg aagatcagga ggagcgccag caggtgaatg tagacgccga
    18481 gcgtcgccgc gccgtaggcc cgtgcgattc cgacgttgat ggcgaacgag aagacgggga
    18541 cgaggacccg tgccgccagc atcgagatcc cgtttgcgag gagcgaacgc accgtcagct
    18601 cttcggaacg ctctcggggt cggaccgcga cgggcccggg agcggctccg cctccacgac
    18661 gagccgctcg cccaggccgg cccgctcgag ggagcggacc aagggccgga aggcgaggcg
    18721 cgaggcctcc gtcaggacgc gcttcggccc ggtgggccgg cgatacgacg ccgggtccga
    18781 cacttcggcc gggccggctc cgggcggggg cgcctcgtgc cggggctggc gaacgagggc
    18841 cggcaggagc cgcaggcagt tcagcgcggt ccggaggctc cagaggggct cccagagggt
    18901 cgtcacgcga acgggacgga acccggcctg cgagaggagc ttcgcgagga ccgagggcgt
    18961 gtagtggaag acgtgctggt ccggccgaag gtcccgccac cgcggtccgt ggacgcggga
    19021 ggcgagggac gagaagttgg ggacctccac cacgaggagg cgcccgaccc ggggagccac
    19081 ctcgtccctc aggaacggca ggggctcgtg gatgtgctcg aggacgtggt ggagggtgac
    19141 gacgtcgaac cgccggccgg ccggaaggcc gtcgagggag ctcgcgacgg ggacgccgag
    19201 cagggccgac gtgcgctcgc gggcgaaatc cgcgacctcg accccggcga cctcccaacc
    19261 ccgggcctgg gcgcgagcca ggaaggcgcc gctggcgcag ccgatgtcga gcacgctgcc
    19321 cttcagccca cgtgcctcga tcctctggag ctcgggctcg aaacgcgctt cgtcgtgggc
    19381 gcgggcgaat tccgactcga agttgtagcc ggccgtgccg tcctcggtga agtaggtctc
    19441 gtcgtagacg aactcgggca gctccgaggt ggcgtggagc ccgcaggccg tgcagcgcga
    19501 caggagccca ccgtagatcc tctcccattc gggatgcggg cagaggggag cccaggacga
    19561 gctcacgtcg gggagaacga tcgtcgtcac gcggggacga cctcctgatc gggcgttgta
    19621 ccggcggacc cgacgcccag aatgccgaag aggagaccga ggtggaccca gacgatcgac
    19681 cggagcggct cgatccgatg gctgaagtcc gcgacctggg agacggcgaa gcccaggaac
    19741 cccgcgagaa gcgccgcggc gacccaggcg agggccgggt ccgcgacggt ccgcagccgg
    19801 cgcagcgcgg acacgacgac ggtgaagatg aggaggagga agagcaggcc cccgggaagg
    19861 ccggtctcgg cggcctcgag gaggtagagg ttgtggacgg tcgcggggaa gtaccgcttc
    19921 acgttccgcg ggtcgtagcg ctccatgacg tggatgaagc tgttcagccc gactccgccg
    19981 accgggtggc tcccgatcat tcgcagggcg atcgcgttga gctcgtaacg gacctcgacg
    20041 ttccccgcct ggctccgggc gagacggtcg tagaccgccg gcccgaagag gacgccgaga
    20101 gccaggacga ggcccgcggc ccccgcgaga gcgcgccgga ccgacctccg ggccgcgcca
    20161 gcgaggagcc cgcgacgcca ggcgaggagg agcagggaga tcgatgcgat cgcggcggag
    20221 aaccaggcgc cgcgggagag ggtcaggatc agccctgcgg ttccgaggga gaacgtcccc
    20281 gcaacgaggg ccctcttcag accgccggag cagacgaagt acccggcggc caggacgggg
    20341 agcgcgagca cgaggaagtt cgcgtagacg atcgggtggt cgaagagccc cgagggcctg
    20401 agccggacgg cccccgacac gagggtctcc cggagcacct cggtcccgtg gaagaaggag
    20461 cgcccgacgg ggcgtcccgt cgcggcctgg aggagcgcga agatcgtgtg gacgctgacg
    20521 gcgcacgaga ggagcacgag gaaaggagtc agcagggggc gccggaagag cgcggccgtg
    20581 gcgaacgcca ggaagagggc gtggacggcc gacgagagct cgaagaggcc gaggtcgcgg
    20641 tgggagcccg acaggacgag cgacatcgag taccaggcca gcagcaggcc ataggccagg
    20701 aggacggcgg cgggaacggc gttccgtcgt cgcccccaca ggagggcggc caggaggagc
    20761 ggggctgcca gggagaggaa gaggtccgaa agagagagcc ggagtccggg ccagcccccc
    20821 acgtgaggct ggtagaagaa gtgcttcgcg agcgggatcg aaacgacgcc gacgaagccg
    20881 aggaagaggc cgtccgccat tcgcgccgga gagaaggcga cgagggccgc gagcgcggcg
    20941 gcgcccatgg aggccgcgag gatcaggaac gccgtgcggg aaggtgccac ggcggcgacg
    21001 gcgaacggga gtcctcccgc gaggagtccc gggaggaccc aggacacgcg acgcccggag
    21061 ggggctgcag ccgtgctctc ggatgccgtc gtcatcggtt ttctggccct cgttcgtccg
    21121 gcgcccgtca gggccgatcc agctcttgca tcaggtagag gaacccggat ccgacggcgc
    21181 cgacggagag gaggtgatag gcctcccgga cgtggaggag agtgtccgtc gcgccggccg
    21241 cagtcccggc cgggtcgcac gggtcggtca cgtagacgtc gacggggtgg ccggagccct
    21301 gacggagaac ggagtagagg acctgctggt cgagcgggac gacgttgtcg gagcccgccg
    21361 acacgacgag cgtcggggcg gtctggaagg ggacgccggg gatcggcgag cggacctcgt
    21421 actcggcgtc ggtgcagtcg acgtgaccgc agatcatctg gtcgagaacg tcggccagcg
    21481 ccgggcctcc cacgtcccag agccggctgc cgccctcgca gagcgactcg gtggcggggg
    21541 cccaggacgt gcggtgaaag gccacgaggc ccttccagtc cgtcggcggg acataggcca
    21601 cgacggcccg caatccctcg atctgaggcg ccgcgcggag gacgacacag ccgccggtgg
    21661 atccgccgac gagcgcgacc cggtcggggt cggtgacctc gagcgagcga acgagcgcgg
    21721 ccgccgcccc gacgtcgtcc gcctccccga ggcagagctc gggggcccct tctccgccgt
    21781 cctggccgcg gagcgaagga gcgaaggccg tcctgccgta ccgcccggcc cagtcgatgc
    21841 aggtctgcac catgtcaccg tcggcgttcg tgaagatccc tccgaaccct ccatggacgt
    21901 gaaggaccgt gctgtggggg gtcccgtcgt ccggatacgc gaggagcccg gcgacgacca
    21961 gcccgccgga ggcgtaggcg acgacgtcga gtgcgacctg gtcgacggtc gcgatcctga
    22021 ggcggccgac gagggccccg tcgtgcagcg tcgtcgaccc cgagggctgc ggcgacgtcg
    22081 cgagggacct ctccagggcc ttctcggcgc tggcgaaggc ggccaggagc gaagcccgaa
    22141 cgtcggcccg catcgacccg ccgtggaggc tccctccccg gctttccgcc agcgaagcgc
    22201 gcgcctcgtg ggcgaaggcc atcaggaccc gctgcccgcg cgccacctcg tccgatcgag
    22261 gtccccaggg cacgcccgat gccgggagcc cggagccggg gccgagggcg aggaacagca
    22321 gggccgctcc ccaggagaca aatagtcgca tttttgaact gtagcacggc tcaccgaacc
    22381 ctccgggatg gtcccgacct ccctgcggga ctcccgccgg ggcggcgccc gctccggcgg
    22441 accaccggcc cgacattctc gagtcctccc cggggacacg cttcgcgtgc gcgggtagaa
    22501 tcccgaggtt tcgaagccga gccttgcagc gattgagcta cccaacctca ggaggactcc
    22561 catgaccagc aaatccgcgc cctccgcgcg cgggacgaag tacgtctact ccttcggcgg
    22621 cggatccgcc gagggcgatg gcagccagaa gaacctcctc ggcggcaaag gcgcgaacct
    22681 cgccgagatg gcgcggatcg gcctcccggt ccccccgggg ttcacgatca cgaccgaggt
    22741 ctgcacgcac ttctacgcga acggccggag ctacccgaag ggcctcgaga aggaggtcgc
    22801 gcagcacctc gccggcgtcg agaagaagct cggcaagaag ttcggcgacc cgaagaaccc
    22861 gctcctcgtg tcggtccgtt cgggcgcgcg cgcctcgatg cccgggatga tggacacgat
    22921 cctgaacctc ggcctgaacg acgtgacggt cgaggggctc gcgaaggcgt cgggcaaccc
    22981 gcgtttcgcg tgggactgct accggcgctt cgtcgccatg tacggcgacg tcgtcctcga
    23041 cctcaagccg gtcggcaagc acgagcggga cccgttcgag gtgatcctcg aggcgaagaa
    23101 ggagaagttc ggagcgcacc tcgacaccga gctgacggtc gaggcgctga aggagctcgt
    23161 cggcgagttc aagaaggcga tcaaggagaa gac
//
LOCUS       DAOONL010000054        22789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2165_length_22789_cov_18.142606, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000054 DAOONL010000000
VERSION     DAOONL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_22789_cov_18.142606"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..542
                     /locus_tag="PLP50_09680"
     CDS             93..542
                     /locus_tag="PLP50_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51862.1"
                     /translation="MLSTLNLRALGWALLASALLAASVVAGSRNLQNFDGALAAYLFA
                     TLFAAFGIVYRYAVWLQRPPTRLYWRAGWRLLFSPRFPAYLAELARRLVDELALQRFI
                     WKRSPKRGLAHVLLAWGCLSAFAITVPLVFGWVHFTLKNGTIDVYCP"
     gene            1234..2718
                     /gene="istA"
                     /locus_tag="PLP50_09685"
     CDS             1234..2718
                     /gene="istA"
                     /locus_tag="PLP50_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPA51863.1"
                     /translation="MIPQETVVEIRRLFYGEHFPVGTIAATLGVHHETVERAIGNAEF
                     HGPRPAERASVTAPFREFLAGTLGQYPRLRATRLYEMVRQRGYAGSVVQLRRAVRELR
                     PVAPEAFLKLRKFPGEEAQVDWAHFGTVKVGRAERRLSCFVLTLSYSRALYLEFFFDQ
                     HLESFLAGHVNALTDLGGVPRTFLTDNLASAVLERAGSHVRFNPRYSELCAHYHAAPR
                     PCAVGRGSEKGRVERAIQYVRHGFFAARPFTTLEDFNRQALLWRNEVAHARPWPGDDR
                     ITVAEAFAQERSALLPLPAHPFETTRLVPAARSKTIYVRFDLNDYSIPPEAVGRPLTL
                     AVTSTSVRVLSGVEEIARHPRCWDRHAVVEVAAHKDALLREKRKALGSTGSGRLSACV
                     PKAEVFLSAAVKRGASPGVETRRLLALLRDYGAEALAWAVEEALSRGTPRAASVHFLL
                     EARRRSRRFRTAPPLDLRSRPELDQMHVTPHALETYDDLADSED"
     gene            2693..3484
                     /gene="istB"
                     /locus_tag="PLP50_09690"
     CDS             2693..3484
                     /gene="istB"
                     /locus_tag="PLP50_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPA51864.1"
                     /translation="MTSPTPKTDLPAALSSLGLHATAASLDDFLSRATRAHWPVRQLL
                     EELVRVELDARASRSLQRRLLRSRIGPFKPLADFDWNWPKKIDRPLIERALTLDFLRD
                     GRNLVLVGANGLGKTLLAKAIAHHAVLSGYSVVFRTAAELLADVQATDSPQLRRKKLA
                     KYTRPQLLCLDEVGYLSFDDQAADLLYGVLNPRYEASRSTLVTTNLAFKDWPSIFPNA
                     TSLVTLIDRLTHHADVTLITGQSYRVRESEQEAAARLNTRPPKPS"
     gene            3481..3876
                     /locus_tag="PLP50_09695"
     CDS             3481..3876
                     /locus_tag="PLP50_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51865.1"
                     /translation="MTETLCARDYVAAVAAVLADLPEPRPRFRPADRRLALDLRRRGV
                     PIPTLRAAVRLATLRRLTRDPARPPLPPVRSLSYFLPVLDELLAAPPAPACLAYIDAR
                     LARLKPAPTPSPGLGPSAPVQNPAFSSER"
     gene            4065..4490
                     /locus_tag="PLP50_09700"
     CDS             4065..4490
                     /locus_tag="PLP50_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51866.1"
                     /translation="MPLVLLVLISVTGLGLTWDYELMQGRAHAFMAISHAITVILFLL
                     WLPFGKFFHVFQRPAQLGVALYQREGEAGEQAVCPHTGEAFASRMHVEDLKQVTAELG
                     YDYRLAGGGSHLDLSPQGKRAALAKAHLAARTESGAFFG"
     gene            4505..6763
                     /locus_tag="PLP50_09705"
     CDS             4505..6763
                     /locus_tag="PLP50_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HPA51867.1"
                     /translation="MARLPVSPEALVAQFGPSLGFPPKEGFPGRDEPDRVVPTHCCFC
                     GMQCGIKLLVKDNRIVGFDPWEEFPFNEGRLCPKGVQRYLQNNHPDRLLSPLERVEGQ
                     GFRPLSWDAALDRTASEVRRIQEKHGKDAFAMLSGVSLTNEKSYLVGKFARLALGTRN
                     LDYNGRLCMVSAGAGNKKAFGLDRASNGYADLAKAEVILVAGANVSETFPTLTHWIWQ
                     ARDNGAKLIVVDPRMTPLARTADLHLPVRPGRDSALFGAILKQLVDRDQLDHDFIANH
                     TSGFAEAAEAVKGYDLAWAEEVTGIPRRKIEAAAEWWGKAKTSFLLHARGIEHHTKGV
                     ENVVSAINLVLATGRIGKPYCGYGTITGQGNGQGGREHGHKCDQLPGNRDIENSKHRE
                     WIAGVWGVPEPEIPGKGLSAYEIVEAIHRGEIKGLLSICFNPLVSLPNASFVREALEK
                     LEYFAAIDFFLSETARHADVVLAGSLHEEEEGTVTTAEGRVTRVRAAIDPPADAWRDT
                     DVLLDLARRLGREELFDFAAPAPLAGTREGSLFTAKRIFDELRAASKGGTADYFGITY
                     EKIEANLGVFWPCPSVDHPGTPRLWEDRKFATPDGRAHFNAVRYRDPGEVTDPDFPVI
                     LTTGRVVSQYLSGTQTRRIGKLVEQYPEPLLEIHPALAKTLGIADRDLVRVETRRGAA
                     EFPAQLVTTIREDTVFIPYHWPGRKSANLLTSGLLDPVSKIPEFKVSACRLVPTGRKA
                     PSAAEAKAHASV"
     gene            6777..7361
                     /locus_tag="PLP50_09710"
     CDS             6777..7361
                     /locus_tag="PLP50_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPA51868.1"
                     /translation="MTTTASRSHEEFFLDPQRCIGCHACEMACAECETNGQLAMIHVD
                     YVDRAMSPQTSVQVCMHCEEPTCARVCPADAIAKDELGVVHSANAARCIACSNCVLAC
                     PFGVPKKIEPMKLMMKCNLCYDRTSAGRRPMCATVCPSGALWYGPRERMAEMRPNSSP
                     VNEFRFGLQRVKTRVNVMMPKGSTELVVDGTEAP"
     gene            7358..7915
                     /locus_tag="PLP50_09715"
     CDS             7358..7915
                     /locus_tag="PLP50_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPA51869.1"
                     /translation="MSDRPEGLPARPAWEVDFPLERVEAQHVSRREFAKFLVLASGGL
                     AVGSGWVAAKDALFPPAAIPGPVRLAALSEIPVGGMTQFTIPGLDQPGILIRLEEGLF
                     RAFEQKCTHLSCAVFFDPGSRKIECPCHNGAFDPMSGEVLQGPPPRPLRSFPVEVRGD
                     EVWLVPPEAPAEAAAPPAAPETEGA"
     gene            complement(8127..8345)
                     /locus_tag="PLP50_09720"
     CDS             complement(8127..8345)
                     /locus_tag="PLP50_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51870.1"
                     /translation="MTGVAVSQKRCPAEIRKPASGSLIAIADHMNQTEKASVRETALI
                     ASVRQASAASGKLSAAGRAATARIGRGR"
     gene            8495..9445
                     /locus_tag="PLP50_09725"
                     /pseudo
     CDS             8495..9445
                     /locus_tag="PLP50_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9620..12136
                     /locus_tag="PLP50_09730"
     CDS             9620..12136
                     /locus_tag="PLP50_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51871.1"
                     /translation="MRAAFRTPVWFRSALPVILAFGIGGPARGDVLSWVATDLTGVDV
                     MDVAIEPGGSGRIWAATAGRGLLLSTDGGKTFAPSPSLGKDARVFGIDFDPENWSVVY
                     AATADGLYRVTAATAERLWLRDPDPTAMWVSVDPHDPRVLLLGASGGCIYRSADRGIT
                     WSPTRCGTHPYRSRVGRALFDPSGTGLVLATAYDWYPGSRLLRSGDRGATWSYVDPAQ
                     VAPTWPNLAVDPEPPGEGNLSHRVWCASTLSPGLLVSRDFGLNWSSVVAAKPGAKPEA
                     FGALALTASGRILSAGPGGVFQTDRTGSPWAPSTVGRPSGEVRRIVALDDAGAELLLA
                     TSTGLYRTERTAEVPAAPVLLPTIVDAVGRGGARFTTSFTLYNASDGMAEGTVDYVAS
                     ESAGSAGSGSFPFRIPAREQLVVGDASTWLRAAGLAVPEATPDQPQVGTLTIRSSDGL
                     WGGEVVAVARVMTPSGPGLSGTGLWSLPASSLTTTPSYLPGLRETAEERTNVAVANAG
                     TAGTIKLRATFRHAAGTFTREAELGPGQWAQWNAPLGSEGVPWADATIERIAGDEPYT
                     AYATIVDQSTNDGSFLPARPAPAAGAAVVPVVVEANGFETELVLFNPGSVDATASLVF
                     TDSLDPAPGGIAPTPSLAVPVPARGSVVLPRVIDELRRRGVPIGPTTASQAGPLRVEA
                     AADGHPVPILASARTTIPAPSGGAYGVSYPALSPDELARSEVWVTGLGPASDVRSNLA
                     LLNPGTEVVTLEWTVFPGSPGGWPRGLEGTVTLRPGEWKQILLEDAGDTYRFPVRNVV
                     VRVRKTSGPGPFAAYGVVNDGESPGLGTGDGTYVPMSATR"
     gene            12371..15205
                     /locus_tag="PLP50_09735"
     CDS             12371..15205
                     /locus_tag="PLP50_09735"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA51872.1"
                     /translation="MPRLRIHASPDRFAAGRIVAIPAEGLVLSRARGQGLVIDDPGIA
                     HGHARFDLTVHGLVLTDGGSGLGTWANGVPVTRVSLRPGDQVQVGSTLFVVQGDQRPT
                     PAPASRPGSSPRRRSSPWAACCLSVLALVVLLSAGGAFIVYRYGLPWVNEQLGLEKPA
                     TDAGDGRTDPREEVVPEVAGPPQESGTESTGRVTPGGGHQAFSLGDQVTVVVPPGAVD
                     APADLVIRPADGVPEAGWSAMRAGRVFDVSVSGRTLFKKPLALTLSYDPDLVPKGADP
                     ALSVAAAYYDEGSREWVVVPAAVDTARRRVAFLTEHLCKWTVLYRQLGWETHASRHFN
                     IVHRPVDAIAFLKGGQKGSLFADRFVKDLGEWLDQAYEAYEKQGFTMPGFELVPAEAP
                     LSEARVVPPATLPGFERLIDGAGALILENRKVPIWAFVGSATAYWGSQAQEAAWNPKS
                     GNILFPTGFDSPEVCRHEASHELFHAVQNRYFNIASMAVRRWWVEATADYAAARIAIG
                     ETGPTPNMGAAIKPRYLERCISFLIPDDKGDQPDSFHEYSTAHFVDYLVSRGAGFKAM
                     WDAVARPGLADLTQVLDPLDKHLKERFGEKRGLAAFYADFARFYLFDPASPLRLKAGE
                     TLRSTAATRLDTLDPGASAAFTFSLEPSFTARLWSVKPKWDANRPTRKVKVTAPRRAA
                     GVSLFLYRLPEGRRLAGGGGFEAELGDKPLVLSIGQDDHLLVLAVRAFEGGKASAEVR
                     MEDLAEPAKVKAPPPAPAARRGLELQMVLYVSYDRGYQDEKQRWPASGFQPFENTGWR
                     GFVLSGSGHFTGTRLDGDKESRVEGTRTASAVTKLKWINHRLTKRPNGEKIQEEWWEI
                     ELENIPVTPHDPNEYILEGYDASTGKWNTGFEKSVPRLEHKVVFYPSGTSIALKNVFW
                     QRTMAKSIGDMEGFAVLAERPHIRLYFTGP"
     gene            complement(15340..16035)
                     /locus_tag="PLP50_09740"
     CDS             complement(15340..16035)
                     /locus_tag="PLP50_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPA51873.1"
                     /translation="MTTFLAQMSFTWLALFPILNPPAMAPVFLGLTSAVTDRARNRLA
                     LRIGVYTALFLTVLLLVGGWILKILGISIPVVAIAGGILLFHSAWHMLNKDPRVSEEE
                     QAEIRSSMENKAFFPLTMPVTAGPGAMAVTLSLVPRGHILEASTLLAFLATACGILLA
                     GATVFVFYRFSGPVIRRMGDTGAATISQISAFVLLAIAVQIVWGGVSDLLVQTLEDPA
                     LAEGLKAFAERLR"
     gene            16202..17425
                     /locus_tag="PLP50_09745"
     CDS             16202..17425
                     /locus_tag="PLP50_09745"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HPA51874.1"
                     /translation="MKLFRSSLLISALVLLIAVPATADMRGVPATDERPQATIEGTIT
                     SVNVPIVGGGPIVTLLDGLVAFDATGATVRYFNGYSATTDTLAAGQRVLALLDPATAP
                     LRATTVVILTDRADVTFSGRVGAVDSDAGTLSVLGFTSKVNERTVFGGPFEGIGSKGL
                     EDVRVGDMVFVEAKADAGALLATKVMKLGAPSDSVRRIHGTVASIGETAWTLTLPDGS
                     ALTVKVGAETKVTGAPKVGDTVDVLARPQSDGSLLAVAILKSVPPPVVAFERFEGAVK
                     AIGATTWTIAPKGGGPDRSFAVDGETKILGNPAVGDGVGVLAKKLDDGTWLAVIIAKA
                     MVPGPARTEVEFEGIVKSISPGNPTGVWIVGETKVVVTSRTVVRGEPRVGDTVEVEGQ
                     RNPDGVVQASKIEKR"
     gene            complement(17508..19499)
                     /gene="uvrB"
                     /locus_tag="PLP50_09750"
     CDS             complement(17508..19499)
                     /gene="uvrB"
                     /locus_tag="PLP50_09750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPA51875.1"
                     /translation="MSLFEVVSELTPQGDQPAAIEALVRGFREGREQQVLLGVTGSGK
                     TFTMAKVVEALDRPTLVLSHNKTLAAQLYQEFQRFFPRNRVEYFVSYYDYYQPEAYVP
                     QSDTFIEKETQINEEIDKLRIAATAALFERRDVLIVASVSCIYGLGSPESFGRMAIEL
                     KTGARYGLTWYLKRLVEAQYERTSLDLDRGSFRVRGDVLEIHPSYEDTGVRVEFFGDE
                     IERVSRIDLLTGKVLEPLREFSLYPKSHYVTPEEVRQRALGSIEAELVTRVADLTAQK
                     KLLEAQRLEQRTRFDLEMMREIGYCAGVENYSRHLSGRAAGEPPPTLLDYFPADYLTI
                     VDESHQTVPQLRAMYHGDRSRKETLVEYGFRLPSALDNRPLRFEEWLERVGPRLYVSA
                     TPAAYELQQAGGEVVEQVIRPTGLVDPAVEVRPVKGQVDDLLGRVRERAARGERTLVT
                     TLTKRMAEDLTSYFLELGVRARYLHSDVETLERVQILGDLRRGVFDCLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLVQTAGRAARNLNGLVVFYADVVTDSMRRAMDET
                     ARRRAIQEAYNAEHGIVPETIVKSLDSPLLKMANLDYGDAPALRASGKDALRDDRSIE
                     EEIASLSKKMKEAARELEFETAAQLRDRIRELRAMQVFR"
     gene            complement(19496..19918)
                     /locus_tag="PLP50_09755"
     CDS             complement(19496..19918)
                     /locus_tag="PLP50_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51876.1"
                     /translation="MSSTPPPPISPGGPPATRDEKDPRPGGVLKWGLVGCAGLSVLLI
                     VGMVLFLRKAPQLMESLLGATESQVVSAIAADVPAAEREAFRTEYAAFVETAKAGKAK
                     PDDIQGLQKKIVAALEDRTVSGEELKGLTEHLRSMPKK"
     gene            19981..22398
                     /locus_tag="PLP50_09760"
     CDS             19981..22398
                     /locus_tag="PLP50_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPA51877.1"
                     /translation="MLGRRAAALLVLLLPVLLAAGLGVGAGWATSRLIRVPKVEQLAS
                     YRPDIVTEIRGADGSIVARFAIERRFLVTRDQLPEVLVQAVLAAEDTRFYDHGGVDII
                     RIGGAALRDLATRRLAEGASTITQQLARSVFLTPQKSFARKINEVFLTVEIEKRFSKD
                     QILTMYLNQVYLGHGNYGVEAASTWLFNHPARELTLTEAALLAGLIQRPEAFSPVRNP
                     AGAKSRRDLVLRRMTEVGYIDAATAAAARAEPVVLSRSAREATIGPYFCEEVRQYLEK
                     TYGDRGLYRQGLRVDSTIDPRIQSWAEEELRRGLRRHERRFGFRKPRNLVVEGVDPER
                     YRDPEWDEPEAPGRDARAERAVVLSATRTGAELRVKDRRFTLPARAFRFTRAESPAKA
                     VKRGDVVLVERIEDEKGREETIVWQEPLVEGAVVVLENGTGAVRALVGGYDFSRSKFN
                     RAVQALRQVGSAFKPFVYMTAVEAGFTSADTVLDSPIAITIDPRQPPWRPQNYTRSYG
                     GILTYQHALENSINVPAVRVDLLVGTRKVIETARRLGIRQNLLAYPSLALGSFEVTPM
                     EMTAAFSVFANQGLLFSPRLVERVRDAEGVLLEENPPDPSEAASPVASYVLLNMLSGV
                     TERGTAARARALGLKVAGKTGTTNDHTDAWFVGVTPRHTIGVWVGHDAKKSLGAKSTG
                     GDTALPIWMGIVSRLKEAGLVKPSDDFDVPQGVVFVPFDLATGYRATPDCRRVVLGAF
                     ANGTQPTELCGERPHAVSTLPHYLQKAVYSPKRGEPAGAEVRVDDAPSSGRAGLPRSL
                     GENGPPG"
     gene            complement(22480..>22789)
                     /gene="rplI"
                     /locus_tag="PLP50_09765"
     CDS             complement(22480..>22789)
                     /gene="rplI"
                     /locus_tag="PLP50_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPA51878.1"
                     /translation="RLEQERKRYDVAQAKEKEQAATVAKAFEGLTVTIRKKAGEHDAL
                     YGSVTAAELAEALEAKGITVDRRRIELDEPIKRLGAHTVHVRLHREVIAEMNVEVVAG
                     "
BASE COUNT         3239 a         8403 c         7686 g         3461 t
ORIGIN      
        1 cctcctgacc cggcccggaa tcctccttgc cccatgaccc gcgtcatacg cgggcccttc
       61 tcagcggacc cgggcctccc tagagtccgt ccatgctctc gacgctgaac ctccgcgccc
      121 tcggctgggc actcctcgcc tcggccctcc tcgcggcatc cgtcgtcgcg ggctcgagga
      181 acctgcagaa cttcgacggg gcgctcgcgg cctacctctt cgcgacgctc ttcgcggcgt
      241 tcggcatcgt ctaccgctac gccgtctggc tccagcgtcc gccaacgcgg ctctattggc
      301 gggccggctg gcgcctcctc ttctcccccc gcttccccgc ctacctcgcc gagctcgccc
      361 gccgcctcgt cgacgagctc gcgctccagc gcttcatctg gaagcggagc ccgaagcggg
      421 gcctcgcgca cgtcctcctc gcgtggggct gcctctcggc cttcgcgatc acggtcccgc
      481 tcgtcttcgg ctgggtccac ttcacgctga agaacggcac gatcgacgtc tactgtccct
      541 gatcacctga gatcgcggga ttctgaacaa gagccggctg aagggtagtc agcatcttcc
      601 ccgccgccgc ggagcggcgg cgggcgaggt ggtgtgtcgt aaggtgggcg acggggtcgg
      661 gcggacgaga gaagggcccc gactgttgac gcagccggga cccgagggcc ggggaaaacc
      721 gaagtaggtg cgcagaggtt ttcacggtcg gatctggaag tcgagcccgg cggtgaggct
      781 gtctgcaagg gactggtcgc gttgggcttc ggcgtctgtg cgagaggtgc gagcggccgt
      841 tcgtgatctg ccatgagtgc gaccggggca atgtgtacgg aagtgttgcg tgccaggtct
      901 cggcgcggcg ggagagtcag cggcgctcga atcggcggca ccagcagagc cgggcgggac
      961 ggcacgacca cgccgaccgc caggcccggt accgccggaa agcgcccggc gagaaagtga
     1021 cggatcaggg tagagacgga gaggggaagg cggtcaccgt ggcggcacgg ccggagaggc
     1081 gcccgagcgc tccggcgcgg gaggtgaccg atggacagac ccggtccggt catcgtcgcc
     1141 gggtgtcggc ggcggcgtgc agcgtggcgg agtgcatcgt ctgcggggcg tcccacccgt
     1201 ggacggaccc gttccacgag ctgggagtag gccgtgatcc cgcaagagac cgtcgtggag
     1261 atccggcggc tgttctacgg cgagcacttc ccggtgggaa cgatcgccgc gaccctgggc
     1321 gtgcaccacg agacggtgga acgggcgatc ggcaacgcgg agtttcacgg gccgcggccg
     1381 gccgaacggg cctcggtgac ggcgccgttt cgggagtttc tggccgggac gctggggcag
     1441 tacccgcgtc tgagggcgac gcggctctac gagatggtcc ggcagagggg atacgccggg
     1501 agcgtggtcc agctgcggcg ggccgtgagg gagctgcggc cggtggcgcc ggaggctttc
     1561 ctgaagctgc ggaagtttcc cggggaggag gcccaggtgg actgggcgca cttcggcacc
     1621 gtgaaggtgg ggcgggccga gaggcgcctg tcgtgcttcg tcctgacgct gtcctactcg
     1681 cgggcgctgt acctggagtt cttcttcgac cagcacctgg agagcttcct ggccgggcac
     1741 gtcaacgccc tgacggacct gggcggggtg ccgaggacct tcctgaccga caacctggcc
     1801 tcggccgtcc tggagagggc cgggtcccac gtccgcttca acccgcgcta ctcggagctg
     1861 tgcgcccact accacgcggc cccacgtccg tgcgccgtgg ggcgagggtc ggagaagggg
     1921 cgggtggagc gggccatcca gtacgtgcgc cacggcttct tcgcggcccg ccctttcacc
     1981 actctcgagg acttcaatcg ccaggctctt ctctggcgca acgaggtggc ccacgctcgg
     2041 ccgtggcccg gcgacgaccg gatcacggtg gccgaggcct tcgcgcagga gcgctcggcg
     2101 cttctgcctc tgccggcgca ccccttcgag acgacgcggc tcgtgccggc cgcgcggagc
     2161 aagacgatct acgtccgctt cgacctgaac gattactcga tccctcccga ggccgtcggc
     2221 cggcccctga cgctggccgt gacgtcgacg agcgtgaggg tcctctcggg cgtcgaagag
     2281 attgcccggc acccccgctg ctgggaccgt cacgccgtgg tggaggtcgc cgcccacaag
     2341 gacgccctgc tgagggagaa gaggaaggcc ctcggctcga ccggctcggg gcgcctttcg
     2401 gcctgcgtgc cgaaggcgga ggtcttcctg tcggccgccg tgaagcgggg cgcctcgccg
     2461 ggggtggaga cccgacgcct tctggcgctc ctgcgcgact acggggccga ggcgctggca
     2521 tgggccgtgg aggaggctct ttcgcgggga acgccccgcg ccgcctcggt ccacttcctg
     2581 ctcgaggccc gtcgccgctc gcgtcgcttc cgcaccgccc ctccgctgga cctgcgctcg
     2641 cggcccgagc tggaccagat gcacgtcacc ccccacgcgc tggagaccta cgatgacctc
     2701 gccgactccg aagactgacc tgcccgccgc gctttcctcc ctcggcctgc atgccacggc
     2761 cgcatccctc gacgacttcc tctcccgcgc caccagggcc cactggcccg tccgccagct
     2821 cctcgaagag ctcgtccgcg tcgagctgga cgcccgggcc tctcgaagcc tgcagagacg
     2881 tctcctgcgc tcgcgcatcg gccccttcaa gcccctggcc gacttcgact ggaactggcc
     2941 gaagaagatc gaccgccccc tgatcgagcg agccctgacg ctcgacttcc tccgcgacgg
     3001 ccgcaacctc gtcctggtcg gcgccaacgg cctgggcaag accctcctgg ccaaggccat
     3061 cgcccaccac gccgtcctct ccggctactc cgtcgtcttc cgcaccgccg ccgagctgct
     3121 cgccgacgtc caggccaccg attcccccca gctgcgccgt aagaagctcg ccaagtacac
     3181 ccgcccccag ctgctctgcc tcgacgaggt cggctacctc tccttcgacg accaggccgc
     3241 cgacctgctc tacggcgtcc tgaatccccg ctatgaggcc tcccgctcca ccctcgtcac
     3301 gaccaacctc gccttcaagg actggccctc gatcttcccg aacgccacgt ctctggtcac
     3361 cctcatcgac cgcctcaccc accacgccga cgtcaccctg atcaccggcc agagctaccg
     3421 cgtccgcgag agcgagcagg aagccgccgc tcgcctgaat acgcggcccc cgaagccgtc
     3481 gtgaccgaaa ccctctgcgc ccgcgactac gtcgccgccg tcgccgccgt ccttgccgac
     3541 ctgcccgagc cacggccccg cttccgtccc gccgaccgtc gcctcgctct cgatctccgc
     3601 cgtcgcggcg tcccgatccc gaccctccgg gccgccgtcc gcctcgccac gctccgcaga
     3661 ctcacacgcg accccgctcg ccctccgctc cctccggtcc gctccctgtc ctacttcctc
     3721 cccgtcctcg acgagctcct cgccgcaccc ccggcgcccg cctgccttgc ctacatcgac
     3781 gcccggctcg ccaggctcaa gcccgcgccg acgccgtctc cgggcctcgg cccgtcggct
     3841 cctgtccaga atcccgcgtt ttcaagtgag cgctgacagt ctacgaggcg cacctcctcg
     3901 gcttccacgc cttcgacttc ccgctcggcg gcttcgtcgc gctgaacctc ttccacgccc
     3961 tgaactggtc gtcggtcctc gtcctcctcg gcgtcttcgg gttcctgcgg cgccggatca
     4021 ccgacgccgg gcagatctcg acgcagacct tcgagatgga cctgatgccg ctcgtcctcc
     4081 tcgtcctgat ctccgtcacc ggcctcgggc tgacgtggga ctacgagctg atgcaggggc
     4141 gggcccacgc cttcatggcg atcagccacg cgatcaccgt catcctcttc ctcctctggc
     4201 tcccgttcgg gaagttcttc cacgtcttcc agcggccggc acagctcggc gtcgcgctct
     4261 accagcgcga gggggaggcc ggggagcagg ccgtctgccc gcacaccgga gaggccttcg
     4321 cctcccggat gcacgtcgag gacctgaagc aggtgacggc cgagctcggc tacgactacc
     4381 gcctcgccgg cggcggctcg cacctcgacc tctccccgca gggaaaacgg gccgcgctcg
     4441 cgaaggccca cctcgccgcc cggaccgagt cgggcgcctt cttcggctga gaggagcgaa
     4501 cgaaatggca cgcctccccg tctcccccga ggccctcgtc gcgcagttcg gcccgagcct
     4561 cggcttcccg ccgaaggaag gcttccccgg ccgcgacgag cccgaccgcg tcgtcccgac
     4621 ccactgctgc ttctgcggga tgcagtgcgg gatcaagctc ctcgtgaagg acaaccggat
     4681 cgtcggattc gacccgtggg aggagttccc gttcaacgag gggcgcctct gcccgaaagg
     4741 ggtccagcgc tacctccaga acaaccaccc cgaccgcctc ctctccccgc tcgagagggt
     4801 cgaggggcag gggttccgcc cgttgtcgtg ggacgccgcg ctcgaccgga ccgcctccga
     4861 ggtccggcgg atccaggaga agcacgggaa ggacgccttc gcgatgctct cgggcgtctc
     4921 gctgacgaac gagaagagct acctcgtcgg gaagttcgcc cgcctcgccc tcggcacgcg
     4981 gaacctcgac tacaacggcc gcctctgcat ggtgagcgcc ggcgccggga acaagaaggc
     5041 cttcggcctc gaccgggcga gcaacggcta cgcggacctc gcgaaggccg aggtgatcct
     5101 cgtcgccggg gcgaacgtca gcgagacctt cccgacgctc acccactgga tctggcaggc
     5161 gcgcgacaac ggcgcgaagc tgatcgtcgt cgacccgcgg atgacgccgc tcgcccggac
     5221 ggccgacctc cacctccccg tccgccccgg gcgcgactcc gccctcttcg gggcgatcct
     5281 caagcagctc gtcgaccgcg accagctcga ccacgacttc atcgcgaacc acacctccgg
     5341 cttcgcggag gccgccgagg cggtgaaggg gtacgacctc gcctgggccg aggaggtcac
     5401 ggggatcccg cggcggaaga tcgaggccgc ggcggagtgg tgggggaagg cgaagacgag
     5461 cttcctcctc cacgcgcgcg gcatcgagca ccacacgaag ggggtcgaga acgtcgtctc
     5521 ggcgatcaac ctcgtcctcg cgacggggcg gatcgggaag ccctactgcg gctacgggac
     5581 gatcaccggg cagggaaacg gccagggagg ccgcgagcac gggcacaagt gcgaccagct
     5641 ccccggcaac cgcgacatcg agaactcgaa gcaccgggag tggatcgccg gggtctgggg
     5701 cgtgccggag ccggagatcc ccggcaaggg gctctccgcg tacgagatcg tcgaggcgat
     5761 ccaccggggt gagatcaagg ggctcctctc gatctgcttc aacccgctcg tgtcgctccc
     5821 gaacgcgagc ttcgtccgcg aggcgctcga gaagctcgag tacttcgcgg cgatcgactt
     5881 cttcctctcc gagacggccc ggcacgccga cgtcgtcctc gccggctcgc tccacgagga
     5941 ggaggagggg accgtcacga ccgccgaggg gcgcgtgacg cgcgtccgcg ccgcgatcga
     6001 cccgccggcg gacgcgtggc gcgacacgga cgtcctcctc gacctggccc ggcgcctcgg
     6061 gagggaggag ctcttcgact tcgccgcccc ggcgccgctc gccgggacgc gcgagggatc
     6121 gctcttcacg gcgaagcgga tcttcgacga gctgcgcgcc gcctcgaagg gggggacggc
     6181 cgactacttc gggatcacct acgagaagat cgaggcgaac ctcggcgtct tctggccctg
     6241 cccgtccgtc gaccaccccg gcacgccccg cctctgggag gaccggaagt tcgcgacgcc
     6301 cgacggccgg gcgcacttca acgccgtccg ctaccgcgac ccgggcgagg tgaccgaccc
     6361 cgacttcccc gtcatcctga cgacggggcg cgtcgtctcg cagtacctct cggggacgca
     6421 gacccgaagg atcgggaagc tcgtcgagca gtaccccgag ccgctcctcg agatccaccc
     6481 ggcgctcgcg aagacgctcg gcatcgccga ccgcgacctc gtgagggtcg agacgcgccg
     6541 cggcgccgcc gagttcccgg cccagctcgt gacgacgatc cgcgaggaca ccgtcttcat
     6601 cccgtaccac tggcccggac ggaagtcggc gaacctcctc acctcgggcc tcctcgaccc
     6661 cgtctcgaag atccccgagt tcaaggtgag cgcctgccgg ctcgtgccga cgggccgcaa
     6721 ggccccttcc gccgccgagg cgaaggcgca cgcgagcgtc tgaggcggga ggagcgatga
     6781 cgacgaccgc ctcccggagc cacgaggagt tcttcctcga cccgcagcgc tgcatcggct
     6841 gccacgcctg cgagatggcc tgcgccgagt gcgagacgaa cggccagctc gcgatgatcc
     6901 acgtcgacta cgtcgaccgg gccatgagcc cccagacctc ggtgcaggtc tgcatgcact
     6961 gcgaagagcc gacctgcgcc cgcgtctgcc ccgccgacgc gatcgccaag gacgagctcg
     7021 gcgtcgtcca ctcggcgaac gcggcgcgct gcatcgcctg cagcaactgc gtcctcgcct
     7081 gccccttcgg cgtcccgaag aagatcgagc cgatgaagct gatgatgaag tgcaacctct
     7141 gctacgaccg gacgagcgcg gggcgaaggc cgatgtgcgc cacggtctgc ccgagcggcg
     7201 cgctctggta cggcccgcgc gagcggatgg ccgagatgcg gccgaacagc tcccccgtga
     7261 acgagttccg tttcggcctg cagcgcgtga agacgcgggt gaacgtgatg atgccgaagg
     7321 gctcgaccga gctcgtcgtc gacggaacgg aggcgccatg agcgaccgcc ccgaaggcct
     7381 tccggcccgg ccggcatggg aggtcgactt ccccctcgag cgcgtcgagg cgcagcacgt
     7441 ttcgcggcgc gagttcgcga agttcctggt cctcgcctcc ggaggcctcg ccgtcgggag
     7501 cggctgggtc gcggcgaagg acgcgctctt tcccccagcg gcgatccccg gcccggtccg
     7561 cctcgcggcc ctctccgaga tcccggtcgg cgggatgacg cagttcacga tcccgggcct
     7621 cgaccaaccg gggatcctga tccgcctcga ggaggggctc ttccgcgcgt tcgagcagaa
     7681 gtgcacccac ctctcctgcg ccgtcttctt cgaccccggc tcgcggaaga tcgagtgccc
     7741 ctgccacaac ggggcgttcg acccgatgag cggcgaggtc ctccagggcc cgccgccgcg
     7801 gccgctccgc tccttccccg tggaggtccg cggcgacgag gtctggctcg tcccgcccga
     7861 ggccccggcc gaggctgccg ccccgccggc cgcgccggag acggagggcg cgtgaccgac
     7921 ttccgccgcg cccaggagca ggcccacccc tcgaagacga acaccgtcct cctcggggtc
     7981 atcctcctcc tcgtcctcat cgtctcgctc caggtctggc tcctgaccgc cggcctcaac
     8041 acggcgctcg gcggcgaccg ctcgatcgtc tggcccgcct tccacgcctc gctcgtcctc
     8101 ttcctcgcgg gggcggcgtc cctccgctac ctgccgcggc cgatccgcgc cgtcgcggcc
     8161 cgccccgcgg cggagagctt ccccgacgcc gcgctcgcct ggcggacgct cgcgatcagc
     8221 gccgtctccc tcacgctcgc cttctccgtc tggttcatgt ggtcggcgat cgcgatcaag
     8281 ctccccgacg ccggcttccg gatctcggcc gggcagcgct tctggctgac ggcgacgccc
     8341 gtcatcctcg gctccctcct ccggatcccc tacggcgtct tcgtctcgcg cttcggcagc
     8401 cggcggacgc tcgcggccgt cacgctcctc ctcgtcgtcc cctgcgtcgg gacggggatc
     8461 gccgtccagg accccgacga cgccgttcgg cgtcctcctc ttctggtcgg ccgtcaccgg
     8521 catcgccggg gcgaacttcg cgacctccat ggccgtcgtc acgctctggt tcccgaagtc
     8581 gaagcagggc tcggcgctcg ggatcaacgg cctcgggaac ctcggcgtca ccgtcgccca
     8641 gctcctcgtc cccgtcgtca tcgccgccgg gatcttcggc gccctcgggg gcgggtcgct
     8701 ttccctgcgg ctgaaggcgg gcgggacgca ggccgtctgg ctccagaacg cggccttcgt
     8761 ctggatcccg gtcattcttc tctgcacggc ggcggtctgg ttcggcaccc gggactttcc
     8821 gatggcgccg aagacgatct cctcgcagct cggcgtcgcg cgcgacctcc acacctggac
     8881 gatctcggcg ctctacttcc tgacgttcgg ctgcttcgtg gcgatggggg cctcgctccc
     8941 gctcatcatc cagtcggtct tcgccgacgc ccccggcggc gccccgagcc cgctcctcta
     9001 cgcgccgttc gctccgctcg tcgcgacgct gatgcgcccc gtcggcgggt gggccgcgga
     9061 ccgcttcggc gcggggagga tgaccgcgat cgcgatcggg gtgatggcgg tcggcggctt
     9121 cagcctgacc cggttcctcc gccccgacgc cttcgacggc ttcttcgcga cgatcctcgt
     9181 catctgcgcc gcctccggct tcgggaacgg ctcggtcttc aagatcatcc cgaccgtcaa
     9241 cccgcgcgag gcgggggccg tcatcgggat cgtctcgtgc gtcggcgcct tcggcggctt
     9301 cttcccgccg ctcttcctcg gctggtgcat cgagcgcttc gggagccccg cctgggccta
     9361 cacggcgatg gccgtcgtcg cgctcggcgc cctcgccgtc aacttctggt tctaccagcg
     9421 ggagggctcg ccgacgcgct gctgaggcgc ggtcagggag gagctcgacg acggaccgga
     9481 gcatcccgac aggggcggga cgcggcagcg tccggatcgg gagattccac ccgaagctcc
     9541 tccggggcca gtgtcgccac gtcctcgaga tcggcggcga ggacgtgtga ggtgcgtgtt
     9601 ccggccatca ctccctgtgg tgagagccgc ctttcgcact cccgtctggt tccgcagcgc
     9661 ccttcccgtg atcctcgcct tcggcatcgg ggggccggcc cggggtgacg tcctttcctg
     9721 ggtcgccacg gatctcaccg gcgtcgatgt catggacgtc gcgatcgagc ccggcggttc
     9781 cggccgcatc tgggcggcga ccgccgggcg aggcctgctt ctttccacag acggcgggaa
     9841 gacgttcgcg ccgtcgccaa gcctcggcaa ggacgcgagg gtcttcggaa tcgacttcga
     9901 tccagagaat tggtccgtcg tctacgccgc cacggcggac gggctctacc gggtcacggc
     9961 cgcgaccgcc gaacggctct ggctgcgcga ccccgacccg accgccatgt gggtctccgt
    10021 cgatcctcac gatccccgcg tcctgctcct cggggcgtca ggcggctgca tctacaggag
    10081 cgctgatcgc ggcatcacgt ggagcccaac gcggtgcggc acccatccct atcgaagcag
    10141 agtcggacgc gcactcttcg acccgtccgg taccggtctc gtcctcgcga cggcgtacga
    10201 ctggtacccc ggctcgcggc tcctccgttc cggggatcgc ggcgcgacct ggtcctacgt
    10261 cgacccggcc caggtggcac cgacctggcc caacctcgcc gtcgatccgg agcccccggg
    10321 cgagggcaac ctctcccatc gcgtctggtg cgcctcaacc ttatccccag gcctcctcgt
    10381 cagccgggac ttcggtctga actggtcgtc cgtggtcgcg gccaagcccg gggccaagcc
    10441 cgaggccttc ggtgcgctcg ccctcaccgc ctcgggtcga atcctctcgg ccgggccggg
    10501 cggcgtcttc cagacggacc gcaccggaag cccgtgggct ccctccaccg tcgggcgccc
    10561 ttcgggcgag gtccgccgaa tcgtcgccct cgatgacgcc ggcgccgagc tcctcctcgc
    10621 gacctcgacg ggcctctaca gaaccgagcg aaccgcggag gtgccggcgg cgccggtcct
    10681 tctcccgacg atcgtggatg ccgtcggaag aggcggcgcc cgcttcacga cctccttcac
    10741 gctctacaac gcctccgacg gcatggccga aggcaccgtc gactacgtcg cgtccgagtc
    10801 ggccgggtcc gccgggagcg gctcgttccc cttccggatc cccgcccgcg agcagctcgt
    10861 cgtcggcgac gcctcgacct ggctgcgggc cgcaggcctc gccgtccccg aggcgacacc
    10921 tgaccaaccg caggtcggga cgctgacgat ccggtcgtcg gacggcctct ggggcggcga
    10981 ggtcgtcgcg gtcgcgcgcg tgatgacacc ctcggggccc gggctctccg gaaccggcct
    11041 ctggtccctg ccggcgtcct cgctcacgac cacgccgtcg tatcttccgg gcctgcgcga
    11101 aaccgcagag gagagaacga acgtcgccgt cgcgaacgcc ggcacggcgg ggacgatcaa
    11161 gctccgcgcg acgttccgtc acgccgccgg aaccttcacg cgagaagcag agctcggtcc
    11221 aggccagtgg gcgcagtgga acgcgcccct cgggtcggag ggcgtcccgt gggcggacgc
    11281 cacgatcgag cggattgccg gcgacgagcc gtacacggcc tatgccacga tcgtggacca
    11341 gtccacgaac gacggctcct tccttcccgc gcgaccggct cccgcggccg gcgccgccgt
    11401 cgtcccggtc gtcgtcgagg cgaacgggtt cgagacggag ctggtcctct tcaatcccgg
    11461 ttccgtcgac gcgacggcct ccctcgtttt caccgattcc ctcgacccgg ctcccggcgg
    11521 catcgcgccc acgccatcgc tcgccgtgcc cgttcccgcg cgcggctccg tcgtcctccc
    11581 tcgcgtcatc gacgagctgc gaaggcgcgg cgtgccgatc ggtcccacga ccgcctctca
    11641 ggccgggccg ctccgcgtcg aggcggcggc ggacgggcac cccgttccca tcctcgcctc
    11701 ggcgcgaacg acgattccgg ctccctcggg aggcgcctac ggggtctcct atccggctct
    11761 ctcccccgac gagctggcac gctccgaggt gtgggtgacg gggctcggcc ccgcgtccga
    11821 cgtccgctcg aacctcgctc tgctcaaccc gggaacggag gttgtgacgc tggagtggac
    11881 ggtcttccct gggagcccag gcggctggcc ccgaggactc gagggcaccg tcacgcttcg
    11941 acccggcgaa tggaagcaga tcctcctcga ggatgcgggc gacacgtacc gctttcccgt
    12001 ccggaacgtc gtcgtccggg ttcggaagac ctcgggcccg ggcccgttcg cagcgtacgg
    12061 cgtcgtcaac gacggcgaga gccccggcct cggcactggc gacgggacct atgtcccgat
    12121 gagcgcgacc cgctgagccc ctgcagcaga cggaacgcgt cgacgatcct cgtcacctgc
    12181 tcggctggtg catcgagcgc ttcgggagcc ccgcctgggc ctacacggcg atggccgtcg
    12241 tcgcgctcgg cgccctcgcc gtcaacttct ggttctacca gcgggagggc tcgccgacgc
    12301 gctgctgagg cgcaacgact ccttctgcca cgcgatgcgg ccttcggccg ccgcgctata
    12361 tttccgtgga atgccacgct tgcgcattca cgcctcgcct gatcgcttcg cggcaggtcg
    12421 catcgttgcg atcccggcgg aaggcctggt tctgagcagg gcccgcgggc aggggctggt
    12481 gatcgacgat ccagggatcg cccacggcca cgccaggttc gacctcaccg tccacggcct
    12541 cgtcctgacc gacggaggga gcggactggg gacctgggcc aatggcgtgc cggtcacccg
    12601 ggtgagcctg cgccctggag accaggtgca ggtgggctcg acgctgttcg tcgtacaggg
    12661 cgatcagagg cccactccgg cgccggcttc gagaccgggg tcttccccgc gtcgccggag
    12721 ctctccctgg gccgcctgct gcctctcggt cctcgccctg gtggtcctgc tgtccgccgg
    12781 cggtgcgttc atcgtctacc ggtacggact gccctgggtg aacgagcagc tcggactgga
    12841 gaagcctgcc acggacgccg gggacggccg cacggatccg agggaggagg tcgtcccgga
    12901 agtcgccggc ccgccgcagg agagcggaac cgagtccacg ggacgcgtca cgccgggagg
    12961 ggggcatcaa gccttctccc tgggagacca ggtgacggtg gtcgttccgc cgggggcggt
    13021 cgacgctcca gccgatctcg tcatccgacc tgcggacggg gttccggagg cgggttggag
    13081 cgcgatgagg gcggggaggg tcttcgatgt ctccgtgagc ggccggacgc tgttcaaaaa
    13141 accgctcgcc ctgaccctct catacgatcc cgacctcgtt ccgaagggcg cagatccggc
    13201 tctctccgtg gccgccgcgt actacgacga gggctcgaga gagtgggtcg tggtgccggc
    13261 cgccgtggac acggcccgga gaagggtcgc cttcctgacg gaacacctgt gcaagtggac
    13321 cgtcctgtac cgtcagctgg gatgggagac ccacgcttcc aggcacttca acatcgtcca
    13381 caggcccgtt gacgccatcg ccttcctcaa gggcgggcag aagggctccc tcttcgccga
    13441 ccggttcgtc aaggacctcg gggagtggct ggaccaggcc tatgaagcct acgagaagca
    13501 ggggttcacc atgccgggat tcgagctggt ccctgcagag gcccccttga gcgaggcccg
    13561 cgtcgtgccg cccgccaccc tgccgggctt cgagcggctg atcgacgggg ccggggcgct
    13621 gatcctcgag aatcggaagg tccccatctg ggcgttcgtc ggttccgcga cggcctactg
    13681 gggcagccag gcgcaggagg cggcctggaa ccccaagtcg gggaacatcc tgttccccac
    13741 gggcttcgac agcccggagg tctgccggca cgaggccagc cacgagctgt tccacgccgt
    13801 tcagaaccgg tacttcaaca tcgcctccat ggcggtgcgc cgttggtggg tggaagccac
    13861 ggccgactac gcagccgccc gcatcgcgat cggggagacc gggccgacgc ccaacatggg
    13921 ggcggccatc aagcctcgat atctggagcg ctgcatcagc ttcctcatcc cggacgacaa
    13981 aggcgaccag ccggactctt ttcacgagta ctccaccgcc cacttcgtgg actatctcgt
    14041 gagccgcggc gccgggttca aggcgatgtg ggatgccgta gcccggcccg ggctggcgga
    14101 cctcacccag gtcctcgacc cgttggacaa gcacctgaaa gagcgcttcg gagagaagcg
    14161 ggggctcgcc gccttctacg ccgacttcgc gcgcttctac ctcttcgacc ccgcgagccc
    14221 ccttcggctg aaggccggcg agaccctccg ctccactgcg gccacccggc tggacaccct
    14281 cgacccgggg gcttccgccg ccttcacttt ctccctggag ccgtccttca ccgccaggct
    14341 gtggagcgtg aaaccgaaat gggacgcaaa ccgccccacg cggaaggtca aggtcaccgc
    14401 ccctcgccgc gccgccggcg tctccctgtt tctctaccgc ctgccggaag gccggcgtct
    14461 ggccggcggg ggcggattcg aggcggagct gggggacaag cccctggtcc tctccatcgg
    14521 ccaggacgac cacctcctgg tcctcgcggt ccgggccttc gagggaggaa aggcctcggc
    14581 cgaggtgcga atggaggacc tggccgagcc ggccaaggtc aaggcaccgc cgcccgcgcc
    14641 ggcggcccgg cgcggcctgg agctccagat ggtcctctac gtctcctacg accgggggta
    14701 ccaggacgag aaacagcgat ggcccgcgtc cggtttccag ccgttcgaga acaccggctg
    14761 gcgagggttc gtcctcagcg ggtcggggca tttcacgggc acccgcctcg acggcgacaa
    14821 ggagagccga gtcgagggaa cccgcaccgc ctctgccgtg acgaaactga agtggatcaa
    14881 ccaccggctc acgaaacggc cgaacgggga aaagatccag gaggagtggt gggagatcga
    14941 gctggagaac atcccggtca ccccccatga tccgaacgag tacatcctcg agggctacga
    15001 cgcttccacg gggaaatgga acacgggctt cgagaaatcc gtccccaggc tggagcacaa
    15061 ggtggtcttc tacccatccg ggaccagcat cgcgctgaag aacgtcttct ggcagaggac
    15121 catggccaag tccatcggcg acatggaggg gtttgccgtc ctggccgagc gcccccacat
    15181 ccgcctctac ttcaccggcc cctgaggtgt ccgccgcacc tcgggctcta cgggttcgga
    15241 cacctcacgt ccggaacgcc cgggccggac gcctcctgcc ggcggctgcc agctgtcccg
    15301 ggtgagtcgc gggagagctt catgccccct gttccgctcc tagcggagcc gttcggcgaa
    15361 ggccttcagc ccctcggcga gcgcggggtc ctcgagcgtc tggacgagga ggtcgctcac
    15421 gccgccccag acgatctgga cggcgatcgc gaggaggacg aaggcgctga tctggctgat
    15481 cgtcgcggcc cccgtgtcgc ccatccgccg gatgaccggc ccgctgaagc ggtagaagac
    15541 gaagaccgtc gcgccggcga ggaggatgcc gcacgccgtc gcgaggaacg cgaggagcgt
    15601 cgaggcctcc aggatgtgcc cccgcgggac gaggctgagc gtgacggcca tcgccccggg
    15661 ccccgccgtg acgggcatcg tgagcggaaa gaaggccttg ttctccatcg aagagcggat
    15721 ctcggcctgc tcctcctcgg aaacgcgcgg gtccttgttc agcatgtgcc aggcggagtg
    15781 gaagaggagg atcccgccgg cgatcgcaac gaccgggatg gagatcccga ggatcttcag
    15841 gatccacccc ccgacgagga ggaggaccgt caggaagagg gccgtgtaga cgccgatccg
    15901 gagggcgagg cggttccgcg cgcggtcggt cacggcgctc gtcaggccga ggaagaccgg
    15961 agccatcgcg ggcggattga ggatggggaa caaagccagc caggtgaagg acatctgggc
    16021 gaggaaggtc gtcatgcagc tcccgcggtg gcccggcgcc cgcgccgggg atgctaacgc
    16081 agggcgccga gctccgtccc gccggatcga agcgttaggc ttccgtggcc gccgacactt
    16141 ccccgagaga cgaaaacgat tccggcggat ggaattctgt ccgacccaag cgaggttgcc
    16201 gatgaagctc tttcgatcgt cgctcctgat ctccgccctg gtcctcctca tcgcggtgcc
    16261 tgccaccgcc gacatgcggg gcgtccccgc caccgacgag cgcccgcagg cgacgatcga
    16321 ggggacgatc acgtccgtga acgtcccgat cgtgggcgga gggccgatcg ttaccctcct
    16381 cgacggtctc gtcgcgttcg atgcgacggg cgccacggtc cggtacttca acggatactc
    16441 cgcgacgacc gacactctcg ccgcgggtca gcgcgtcctc gccctcctcg acccggccac
    16501 cgccccgctg cgggcgacga ctgtggtcat cctcacggac cgggccgacg tcacgttctc
    16561 cggccgggtc ggcgccgtcg actccgacgc cgggaccctg tcggtcctcg gattcacctc
    16621 gaaagtgaac gagcggaccg tcttcggcgg gccgttcgag gggatcggct cgaagggact
    16681 cgaggacgtc cgcgtcggcg acatggtctt cgtggaggcg aaggcggacg ccggcgcgct
    16741 cctcgcgacg aaggtcatga agctcggcgc gccctcggac tcggtccgcc ggatccacgg
    16801 gacggtcgcc tcgatcggcg agaccgcgtg gacgctcacg ctccccgacg gctccgccct
    16861 cacggtgaaa gtcggagcgg agacgaaggt caccggcgcc ccgaaggtcg gcgacaccgt
    16921 cgacgtcctc gcccgtcccc agtccgatgg ctccctcctc gccgtcgcga tcctcaagtc
    16981 ggtcccgcct cccgtcgtcg cgttcgaacg tttcgagggg gccgtgaagg cgatcggggc
    17041 gacgacctgg acgatcgccc cgaagggcgg cggccccgat cggtcgttcg ccgtggacgg
    17101 ggagaccaag atcctgggaa acccggccgt cggtgacggc gtcggggtcc tcgcgaagaa
    17161 gctcgacgac ggcacctggc tcgccgtcat catcgcgaag gcgatggtcc ccgggccggc
    17221 gcggaccgaa gtcgagttcg agggcatcgt gaagagcatc tcgccgggca acccgaccgg
    17281 cgtctggatc gtcggggaga cgaaggtcgt cgtgacgagc cgaaccgtcg tgaggggcga
    17341 gccgagagtc ggcgacaccg tcgaggtcga agggcagaga aacccggacg gcgtcgttca
    17401 agcctcgaag atcgagaagc gctgacggtc cggaggccgc tccggcgagg aaggggcggg
    17461 ggcggctcgg ccgcccccgc cccttcacat tccggggctc cctcccgtca gcggaagacc
    17521 tgcatggccc ggagctcgcg gatccggtcg cgcagctgcg cggccgtctc gaactcgagc
    17581 tcccgggccg cctccttcat cttcttcgag agagaggcga tctcctcttc gatcgagcga
    17641 tcgtcgcgca gggcgtcctt cccggaagcc cggagcgccg gggcgtcgcc gtagtcgagg
    17701 ttcgccatct tcaggagcgg cgagtcgagc gacttgacga tcgtctccgg gacgatcccg
    17761 tgctcggcgt tgtacgcctc ctggatggcc cggcggcgcg cggtctcgtc catcgcccgc
    17821 ctcatcgagt cggtcacgac gtccgcgtag aagacgacga ggccgttcag gttccgagcg
    17881 gcgcgcccgg ccgtctggac gagcgaggtc tccgatcgga ggaacccctc cttgtcggcg
    17941 tcgaggatcg ccacgagcga gacctcgggg aggtcgagcc cctcgcgcag gaggttgatg
    18001 ccgacgaggc agtcgaagac gccgcgccgg aggtcaccga ggatctggac ccgctcgagc
    18061 gtctcgacgt cggagtggag atagcgcgcc cgcacgccga gctcgaggaa gtagctcgtg
    18121 aggtcctcgg ccatcctctt cgtcagcgtc gtgacgagcg tccgctcccc cctcgccgcc
    18181 cgctcccgca cgcgccccag gaggtcgtcg acctgtccct tcacggggcg gacctccacc
    18241 gccgggtcga cgaggccggt cggccggatg acctgctcga cgacctcccc gcccgcctgc
    18301 tgaagctcgt acgcggcggg cgtcgccgag acgtagagcc tcgggccgac ccgctcgagc
    18361 cactcctcga atcggagggg acggttgtcg agcgccgacg gtaggcggaa gccgtactcg
    18421 acgagcgtct ccttccggga gcggtcgccg tggtacatcg cgcggagctg agggaccgtc
    18481 tggtggctct cgtcgacgat cgtcaggtaa tcggccggga agtagtcgag gagggtcggc
    18541 ggcggctctc ccgcggcccg cccggagaga tgacgggagt agttctcgac gccggcgcag
    18601 tagccgatct cccgcatcat ctcgaggtcg aagcgggtcc tctgctcgag ccgctgcgcc
    18661 tccaggagct tcttctgcgc cgtcaggtcg gccacccggg tgacgagctc cgcctcgatg
    18721 gagccgaggg cccgctgccg cacctcctcc ggggtgacat agtgcgactt cgggtagagg
    18781 gagaactcgc ggagcggctc gaggaccttc ccggtcagga ggtcgatccg cgagaccctc
    18841 tcgatctcgt cgccgaagaa ctcgacgcgg acccccgtgt cctcgtacga ggggtggatc
    18901 tcgaggacgt ccccgcggac ccggaacgag ccgcggtcga ggtcgaggct cgtccgctcg
    18961 tactgggcct cgacgagacg cttcaggtac caggtcaggc cgtagcgggc cccggtcttc
    19021 agctcgatcg ccatccgtcc gaacgactcg gggctcccga gaccgtagat gcaggagacc
    19081 gacgcgacga tcaggacatc gcgcctctcg aagagcgcgg ccgtcgcggc gatccggagc
    19141 ttgtcgatct cctcgttgat ctgggtttcc ttctcgatga acgtgtcgga ctgcgggacg
    19201 tacgcctcgg gctggtagta gtcgtagtag gagacgaagt actcgacccg gttccgcggg
    19261 aagaagcgct ggaactcctg gtagagctgg gcggcgagcg tcttgttgtg ggacaggacg
    19321 agcgtcgggc ggtcgagcgc ctcgacgacc ttcgccatcg tgaacgtctt ccccgagccg
    19381 gtgacgccga ggaggacctg ctgctcgcgc ccctcgcgga agccgcgcac gagcgcctct
    19441 atcgccgccg gctggtcccc ctgcggcgtc agctcggaga cgacctcgaa gaggctcact
    19501 tcttcggcat ggagcgaagg tgctccgtca ggcccttcag ctcctccccc gacaccgtcc
    19561 ggtcctcgag ggccgccacg atcttcttct ggaggccctg gatgtcgtcc ggcttcgcct
    19621 tccccgcctt cgccgtctcg acgaaggcgg cgtactccgt ccggaacgcc tcccgctcgg
    19681 cggcggggac gtcggccgcg atcgcggaga cgacctggct ctcggtggcg ccgaggagcg
    19741 actccatgag ctgaggcgcc ttccgcagga agaggaccat cccgacgatc aggaggaccg
    19801 agaggccggc acagccgacg agcccccact tcaggacgcc gcccggccgt gggtccttct
    19861 cgtcccgggt cgcgggcgga ccgcccggag agatcggggg gggcggggtg ctgctcatcc
    19921 tctgcctcct cgcacgtctt ccgacccgga cggggcggag acgcatgtga gaatacctcc
    19981 gtgctcggac gccgcgccgc cgcgctcctc gtcctcctcc tcccggtcct cctcgcggcg
    20041 gggctcgggg tcggtgccgg ctgggcgacg tcgcggctca tccgcgtccc gaaggtcgaa
    20101 cagctcgcga gctaccggcc cgacatcgtc accgagatcc gcggcgccga cggctcgatc
    20161 gtcgcgcgct tcgcgatcga gcggcggttc ctcgtcacgc gcgaccagct ccccgaggtt
    20221 ctcgtgcagg cggttctcgc cgcggaggac acgcggttct acgaccacgg cggcgtcgac
    20281 atcatccgga tcggcggcgc cgcgctccgc gacctcgcga cccgccggct cgccgagggc
    20341 gcctcgacga tcacgcagca gctcgcccgc tccgtcttcc tgacccccca gaagtcgttc
    20401 gcccggaaga tcaacgaagt cttcctgacc gtggagatcg agaagaggtt ctcgaaggac
    20461 cagatcctca cgatgtacct caaccaggtc tacctcggcc acggtaacta cggcgtcgag
    20521 gcggcgtcga cctggctctt caaccacccg gcccgggagc tcacgctcac ggaagcggcg
    20581 ctgctggccg ggctcatcca gcggcccgag gctttctccc cggtccggaa ccccgccgga
    20641 gcgaagtcgc ggcgcgacct cgtgctgcgc cgtatgacgg aggtcggcta catcgacgcc
    20701 gcgacggccg ccgcggcgag agcggagccg gtcgtcctct cgcggtccgc gcgcgaggcg
    20761 acgatcgggc cctacttctg cgaggaggtc cggcagtacc tcgagaagac ctacggcgac
    20821 cgggggctct accgccaggg cctgcgcgtc gactcgacga tcgacccccg catccagtcg
    20881 tgggccgagg aagagctccg gcgcggcctg cggcggcacg agcgccgctt cgggttccgc
    20941 aagccccgca acctggtcgt cgaaggggtc gatcccgagc ggtaccggga ccccgagtgg
    21001 gacgagccgg aggcgccggg gcgcgacgcc cgcgcggagc gggccgtggt cctttcggcg
    21061 acccggaccg gcgcggagct ccgcgtgaag gaccgccgct tcaccctccc cgcgcgggcc
    21121 ttccgcttca cacgggccga gagcccggcg aaggccgtga agcgcgggga cgtcgtcctc
    21181 gtcgagagga tcgaggacga gaaggggcgc gaggagacga tcgtctggca ggagccgctc
    21241 gtcgagggag ccgtcgtcgt cctggagaac gggacgggcg ccgtaagggc cctcgtgggc
    21301 gggtacgact tctcgcgctc gaagttcaac cgcgccgtgc aggccctccg gcaggtcggc
    21361 tcggcgttca agccgttcgt ctacatgacc gccgttgaag ccggcttcac ctcggcggac
    21421 accgtcctcg actcgccgat cgcgatcacg atcgacccgc gtcagccgcc gtggcggccg
    21481 cagaactaca cgcggagcta cggcgggatc ctgacgtacc agcacgcgct cgagaactcg
    21541 atcaacgtcc cggcggtgag ggtcgacctc ctcgtcggca cgcggaaggt gatcgagacg
    21601 gcgcgccgcc tcggcatccg gcagaacctc ctcgcctatc cctccctcgc cctcggctcg
    21661 ttcgaggtga cgccgatgga gatgacggcc gccttctccg tcttcgcgaa ccaggggctc
    21721 ctcttctccc cgcggctcgt cgagcgggtc cgcgacgccg aaggggtcct cctcgaggag
    21781 aacccgcccg acccgagcga ggccgcctcc cccgtcgcct cctacgtcct cctcaacatg
    21841 ctctcgggcg tgaccgagcg cgggacggcg gcccgggccc gcgccctcgg cctgaaggtg
    21901 gccgggaaga ccgggacgac gaacgaccac accgacgcgt ggttcgtcgg cgtgacgccg
    21961 cgccacacga tcggcgtctg ggtcggtcac gacgcgaaga agagcctcgg tgcgaagtcg
    22021 acgggcggcg acaccgcgct cccgatctgg atggggatcg tctcccgctt gaaggaggcg
    22081 ggcctcgtga agccctccga cgatttcgac gtcccccagg gcgtcgtttt cgtcccgttc
    22141 gacctcgcga ccggctaccg ggccacgccc gactgccgtc gcgtcgtcct cggcgcgttc
    22201 gccaacggca cccagccgac cgagctctgc ggcgagcggc cgcacgcggt gtcgaccctg
    22261 ccccactacc tccagaaggc ggtctactcc ccgaagcgcg gggagccggc cggcgccgag
    22321 gtccgtgtcg acgacgcgcc gtcctccggg cgagcggggc tgccgcgctc cctcggggag
    22381 aacggcccgc cgggctgacc gctcgtcccg ggccggcccc ttcacgcgcg aaagccccgc
    22441 gaaggcgggg cttccgttcc gggacgagcc ggggagcgcc tatccggcga cgacctcgac
    22501 gttcatctcg gcgatcacct cgcggtggag ccggacgtgg acggtgtgcg ctccgagcct
    22561 cttgatcggc tcgtcgagct cgatccggcg gcggtccacg gtgatcccct tcgcctcgag
    22621 cgcctcggcc agctcggcag ccgtgacgga gccgtagagg gcgtcgtgct cgcccgcctt
    22681 cttccggatg gtgaccgtca gcccctcgaa ggccttcgcg acggtcgccg cctgctcctt
    22741 ctccttcgcc tgggccacgt cgtagcgctt ccgctcctgc tcgagccgc
//
LOCUS       DAOONL010000055        22342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2239_length_22342_cov_22.302553, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000055 DAOONL010000000
VERSION     DAOONL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22342
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_22342_cov_22.302553"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..571)
                     /locus_tag="PLP50_09770"
     CDS             complement(56..571)
                     /locus_tag="PLP50_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA51879.1"
                     /translation="MSTDPKSIAASLLPEFDLEMAGTRKVLERVKDEDWAFKPHAKSF
                     SMGELAGHVATIPEWLTGTLNAPSFDMAPGGVPAEFSRPASAREALALFLEGVKAARA
                     ALEATADPAFAEPWSLLENGKAWFTMPRSAVVRGFVMNHLIHHRAQLTVYLRLRDVPV
                     PALYGPSADES"
     gene            700..1539
                     /locus_tag="PLP50_09775"
     CDS             700..1539
                     /locus_tag="PLP50_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPA51880.1"
                     /translation="MPRIVVVGSIAIDEVVALRRPLAAGLHLDGKEHGRRVGGGAANT
                     AIPLAFAGHDVVVVSAAGTDADGGTILRRLAEAGVDTSQVARLPGPSTRSLVLVDPAG
                     ERTVVNLHRCREGGPLRRLRTVPADAIYVRSRELDLARILAERLDSALVVAHVPPVVA
                     GARPAHVLVASASDLSPEEGERPWDVLRRAGGETVRHVVVTRGAAGAEAHASDGRLRV
                     PAREAASVDSTGAGDVFAAGLLHALVSGAPMEAALRTAVAWGTASVECEGVPPRERIR
                     ALL"
     gene            complement(1550..1921)
                     /locus_tag="PLP50_09780"
     CDS             complement(1550..1921)
                     /locus_tag="PLP50_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HPA51881.1"
                     /translation="MPHLVLEHSANVPDRPDLRRVLLDLHEALLAAAPFERKDLKSRA
                     VRHDVYAVGDGAADRSFVALSIAILDGRSDDVKQAISDAALEVLVRAFPRLVAEGRGA
                     ISVEVRDLHRASYRRVRVREA"
     gene            complement(1977..3026)
                     /locus_tag="PLP50_09785"
     CDS             complement(1977..3026)
                     /locus_tag="PLP50_09785"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HPA51882.1"
                     /translation="MIKALGYGNVAATAPLERMEIDRRDPGPKDVRIDVLYCGICHSD
                     LHTARNEWHGTTYPCIPGHEIVGRVAAVGSEVKGFKVGDMAGVGCLVDSCRSCSECKD
                     DLEQFCAKGAIFTYNSPDAHLGGMTYGGYTNVIVVDEAFVLKVPAGLDPAGAAPLLCA
                     GVTTYSPLRHWGAGPGKKVGVVGLGGLGHMAVKLAHAMGAHVVLFTTSAGKTADAKRL
                     GADEVVLSKDPEAMKAHAGTFHFILDTVAASHDLDAYTTLLRRDGTLCLVGAPEYPHP
                     SPTVFNLIFGRKSIAGSLIGGIKETQEMLDFCAAKGLTADIETIPVQKVNEAYERMLK
                     SDVKYRFVIDMASLK"
     gene            complement(3189..6077)
                     /locus_tag="PLP50_09790"
     CDS             complement(3189..6077)
                     /locus_tag="PLP50_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51883.1"
                     /translation="MSHPLSRRALLHAGIALSLVASAGPGLAQDVYRFDGGEVTHNRS
                     CGPGVPPTGSTSPIQPDARSVVVSLGPWQCFGVHDGTAAFSFPQTVARKPGTSDQWAF
                     DQPATVSVAWSWASLPSPYEKDAYLLVSQLEPVPACRDYRDSPFDGYPAGSTITPQAA
                     IACPEWRGSIGGASRFAVQYNVHSAFRQRLTSGENGYQSLNVKVFYMRDDGVDLEVTL
                     AHRERPVNDPPVWDVTVRNRGGATANDASLTLWSESTVLKVEPACLQGSPFSPACGLG
                     AVAPGEERKFVVSTDLGRNNDRSADLSARVSTASVDVHLPNNEAFDRQMVENCFERAG
                     TACLFVDLACEATTSRLAEGLQPPGAGLGARLLRSLAESTVDTSIFRRLRDEVFSRSA
                     AGRRTTELYYAHARELATLVAASPSLLSATLANVLAWQPHVRALVTGEGAATAVLTSE
                     QVDGLLGVIAQLESLGSPELAAVLARERAARDLPSLVGKTVHEALAIQQETVPDTVTV
                     PAAASIHGVAPAFFHSDLRVLNPATSGAVTVAARYRCFTGPCPADTEKSFTVNGRELK
                     VFEDAVVSLFGAPETAGTIELTGPVLAESRVYTPTLPAPTSGSDVPGLPVDEAFAESV
                     LLSLSRGAFRTNVGVYNPGPDVAAVTVTLHAPGGTKLGEVKRDVSGRSAVQVNDVFGA
                     AGIAGDVPDVYAVVRADGVRELYSYATVIDNRSQDSVFVKGRNARGADPERVTLPAAA
                     SIHGVPPAFFHSDVRVFNPSPDAAATVTATYRCFTGACPAVMEKSFEVGPREMRVLDD
                     VVATLFSAPETAGAIELSGRVHVESRVYTPERGAPTTGTSIPGQPAAEATTQAALLSL
                     SHSAAPGQGFRTNVGVFNPAPEPHAVTISLRRPDGTQLAVLSRTVPPFLPLQVNDVFG
                     AAGVTQDVPAAWALVSGDGVRGFFAYATVIDNQSQDSVFVKGRPLRAP"
     gene            complement(6330..8174)
                     /locus_tag="PLP50_09795"
     CDS             complement(6330..8174)
                     /locus_tag="PLP50_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPA51884.1"
                     /translation="MRRALHLAFLLFGGPALAAGVVGSGTPASCTEAALAAALAGGGA
                     VTFSCGGGAVTIPVTATKTISSPTPTSVDGGGLVTLDGQDAVRLFYVDYQRQLALRNL
                     TLRRGRVADLGAAVRSHYHERGTAALTIENVRFEDNVCTQAGDDVGGGALYIQGGVST
                     VDGSTFLRNRGGNGGAIGYLGGDGTGPANGLTIRDSTFVGNETQARVSSFGATGGALY
                     VDGAGDGEVRIERCTFRDNVAWAGGAIHSVMYLSPQALVISDSTFLENRATGGNGGAI
                     YHQDGAFAFTGSLVAGNRAFTQGGGLWLYHPTSATVTNVTFTGNRAEGAIAPSGYAIG
                     GGFSIYPSGPNVTLSHLTVAHNHAGWTGGGITVAAPASRVTLRASIVAHNTAANGGNP
                     WNIGKNCDVGSPRMTNGGDVIEYPERNLGDGNDENCVAAPVVVEPLLGPLADHGGLVE
                     TVSLLPGSPAIDAAWAGCASAVDARGAARPAGVRCDVGAFEGEETPTAFHTVTPCRVL
                     DTRLGAATPVAANGSLSFDAAGTCGVPADARALAVNLTVVAPQAGGVLRLWGAGGPTP
                     TAHVLGFAPGKTRASSAIASPGALGRLTVRNDSAQPAHAVLDVSGYFR"
     gene            8292..10112
                     /locus_tag="PLP50_09800"
     CDS             8292..10112
                     /locus_tag="PLP50_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPA51885.1"
                     /translation="MPPLVAAQRLSKAFGARDLFSSLSFGIEPGERIGLIGPNGAGKS
                     TLLSILAGETTPDDGAVVFQGGVSVGLVPQIPSFAEGATVRSVVLEGALAHVSHGEEV
                     AWEAELKTDEALAKLSLTSGGLGPDSLVATLSGGQRKRVALARELVRQPDLLLLDEPT
                     NHLDVESIVWLEELLSRAPFATVTVTHDRLFLTRVATRILELDRRNPGGLLSVPGGFP
                     KWLEAKETLLAAQESREASLRNVLRRETEWLRRGPKARATKQKARIERAEALGEEIET
                     LEERNVSRTATLDFAGAGRAPKRLLVAEGVSKAYGGRTLFAGLDLLLGPGSRVGLIGP
                     NGCGKSTLLRVLLGAERPDAGKVTRADGLSVALFEQGRESLDPDATVKETICPEGDQV
                     EYRGRFLHFRSWLDRFLFSQEQAEMKVSRLSGGEQARLLMARLMLRPAQLLVLDEPTN
                     DLDLATLNVLEEALAEFPGALLLVTHDRAFLDRATDSLLAFDPEGGGGVTTLAGLDQW
                     ERWRKERRESTAKASPKPAPAAPPKPAKRRLGYLEQRELDGMEARILEAEERLAARKA
                     ECERPDVVSDGPRLVALAREIEEAQAEVDRLYARWAELSA"
     gene            10156..11658
                     /locus_tag="PLP50_09805"
     CDS             10156..11658
                     /locus_tag="PLP50_09805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA51886.1"
                     /translation="MQEPPRSDPAVRPAGGTPPTRLPALRREALVTTLLALVWAAAWS
                     LGRIQEYAPHASLWFPPAGLTFAALAVLGPRAVPGVAVAAILVTLRLGEVYDVDYDLE
                     ALLVAGALFAAAHVGAFGLGAAALRRWSGNSVPQAVTAFLVVAPASSALAAVGGAVAL
                     ATAGVIERAEARAILLPWFVGDFVGVVALGPALAAVLERLAATFRLRSTPFFGAVSRL
                     RPAHPGWHRFALLFALCVLPLAGSLVLLGGFGLRGLATAFLVFFAIVPLMWIVHTEGS
                     NRAFGAVAALSVAIAGAGALAGAGDHTTAYQFAMIVLAGAAYFGLAVPSLYLDNEQLR
                     RLATTDPLTGAANRLAFDEAAIRETERARRFGMPLSLVLLDLDRFKAVNDTWGHGVGD
                     AVLVEVVRRLRAELREIDVLARVGGEELAVLLPMTPSPAAADTARRLAAALRARPVTR
                     GAIAVPVTASFGVAQVDPAAGFEAARDRADRALYDAKRLGRDRVETAGGS"
     gene            11669..13222
                     /locus_tag="PLP50_09810"
     CDS             11669..13222
                     /locus_tag="PLP50_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HPA51887.1"
                     /translation="MQHLFEVVNDLFAFFVPVSDLLWDFPKNVPAWAAIPVLGEFSFA
                     MLLLLGTGLFFTLRTGAVQLRRFGESIRIVMDRKASETGISPFAAFMLSSAMRAGPGN
                     IMGVTGAVTVGGPGALFWMWVAGTFGMATGFAEATLAQLFKERKESEFVGGLPFYGMR
                     ILGNRRWVGVTLALLFITYALLNVPSQTFHLFTALGSVASTLAGTTYGRTSGVFYAIG
                     ALLVVSVAALVLGGLKRVIRFTDLCVPFMAVLYCGVVLLLVVLNWRLVPWFFGSVFAE
                     AFSPEAIFGGSFGVALQQGIKRGLMVNEAGQGTITMAAAAADNRHPVEQGLVQSMGVW
                     FDTVVICSMAGFVTVMAKLWAAPDVFDWGARKADKLLVFMSSARHLTPGTGFDPAVQV
                     ALSVSYALFAFTTLIGMIVFAEISANMLSRKPRFILGLRALGALFFVPFGVLTVLADL
                     QLGNIWYVTDLVNILVASANVPILLAGGKHVVAALRHYERTGGREGFVSERDIGLATP
                     YWTEEARKG"
     gene            13320..15008
                     /locus_tag="PLP50_09815"
     CDS             13320..15008
                     /locus_tag="PLP50_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPA51888.1"
                     /translation="MTRLRPRLVSACALLLLLLAATLPAAAAKPAAPAREIDALLVRA
                     IPADGPGVAVIAVKDGKTVFRKARGMASLELGVPLSPESVFRLGSITKQFTAVAVLML
                     AEEGKLSLSDPITKYLPGYPTHGHTITVEHLLTHTSGIRSYTGIPGYMSGPKIRAGLT
                     TAELVEVFSKEPMDFAPGAEWRYNNSGYVLLGAILEKVSGKPYGELLAERIFRPLGMK
                     STRVGDEGPILAKRAAGYTRDGEKVANARFLSMTQPHAAGALVSTVDDLALWDAALYG
                     EKLVKRASLVQAWTPAVTRDGRPTRYGYGFGVSTLRGARSIEHGGGIFGFSTYAVRLP
                     DEKVYVAVLANSDAPAADPGTLGKEIAALLIGKPFPARVPVPVDPKLLERWVGVYRFD
                     PQTTRTITLDGGKLFSQRSGGPRLEVKPLSESEFFFDRSLTRISFVAGADGKAKEALF
                     YPEGADEAEKGVREGDVPAVPSETKVDPALYDLYAGEYELAPGFVLTVRRDGNRLLTR
                     ATGQEELEVFPASETEFFLKVVDARITFVKGTDGKVASLVLRQGGREMPAKRIR"
     gene            complement(15112..16614)
                     /locus_tag="PLP50_09820"
     CDS             complement(15112..16614)
                     /locus_tag="PLP50_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPA51889.1"
                     /translation="MSGPSPLPAAFVRSAAARGEERRAGAPLLAGLLSASSLDLLGNL
                     GQLRLLLLACALALADAFRATGLGRGGLFAIVALVLGPLALSALLFRPFDASCVAPYT
                     SVARNAGAAMPLAAWLGLWGLPAFLAVSLVALEVARRHAASEGGAGRRAWLAAAGLLV
                     LALAFGLASGIPSEIVVLPLASAAAALALAPGGGAAPERRAVLLLGAGALLVTLGVDV
                     LSVGGDRMNSVFKLSFQAWALLAVASGPALAWVVVALRDGERTRPGSWPVTAALLLGA
                     SALYPLTATPARFAERFPRVTPGEACRPFEGALAPPDALGSGAAGRPRGLDGSAFLAD
                     SALCVEGRLVPLRYDGAGFAWLRANERGSPAILEAVLPEYRWGSRFSVHTGLPAVVGW
                     SWHVRQHLAVVPGSVVEKRIAEVERFYRSRLAAPGEGPGERRTTVADVRRLLSRHRVR
                     WVVDGLPERAVYGEEGLAKLSELERLGLLERAYANPGLTLWRVLPDATAR"
     gene            16632..17012
                     /locus_tag="PLP50_09825"
                     /pseudo
     CDS             16632..17012
                     /locus_tag="PLP50_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            17155..17670
                     /locus_tag="PLP50_09830"
     CDS             17155..17670
                     /locus_tag="PLP50_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51890.1"
                     /translation="MATDTPTFTPTFTPTSTPTVTPTVTPTTTMTPVPTAAPDVDLGA
                     TTAVPGQALLASGSVPDTSLSYQLCLIAHATWTLGSPFGGVCTLSAPLTSPTIAFSGV
                     PLGSAPGAGSYDLLLLGPASTVLAADDASAAPGLVVTGLANVPVGGRAGLLGFAFLIA
                     AGAWVLLRQFR"
     gene            complement(17743..18663)
                     /gene="ftcD"
                     /locus_tag="PLP50_09835"
     CDS             complement(17743..18663)
                     /gene="ftcD"
                     /locus_tag="PLP50_09835"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPA51891.1"
                     /translation="MTTLIECIPNISEGRRPEVVEAVRKAAVGAGPGVLLLDWTSDAD
                     HNRSVLTFLGDGPGLLRGMEAMVDEAIRHVDLTKHEGAHPRLGAVDVIPFVPIRGATT
                     ADCVALAKELSARLAAKHGLPIYLYEDAATSEARRNLATIRKGEFEGLAEKMKSPEWK
                     PDFGPEAPHPTAGATVVGARSPLIAYNINLGTADLSIADRIAKSIRHLSGGFRFVKAM
                     GVRLEARGIVQVSINMTNYEKTPLHRVFETVRSEAERFGVPVVGSEIVGLVPQAALVG
                     AAEHYLRLEAPASSQVLENKLLDVAFAGSE"
     gene            18765..20741
                     /locus_tag="PLP50_09840"
     CDS             18765..20741
                     /locus_tag="PLP50_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51892.1"
                     /translation="MPELAAYPGSPALSAEAREKVLQTFRHTLEMARAGRNEDALLGC
                     DFILKMDARFAPARQLLASLRGIAAGTVVDLAEFAPYLGGAAPAAAAPAPVPEAPPAT
                     PPSGYGVPPPPVEVLPAVDPFAAPGAGLDSLSFDDLAANPFAPAPAHDPFAAPAPAAD
                     FSFGDPSPAAAPAADELAPTEAAPDPFAGPGPAGDPFAAPAPAAFPSAPPAASGVDPR
                     IAQFLRQGDEAEARGALQEAIDLWSRVFLIDLSNEDASRRIDTARARQDEAARQLDVL
                     LSEGVQLYEAGNLASARDAFLKVLTLSESDATARNYLNEIDVALASTGGTAAHAPSYA
                     PAPVPAGPVEPYLRDELESPAGPAFADGLPSLDETPLGEERLGGGVPAPAPRVEAPAE
                     KGGKVDGRILVGGAVLLLLGVAAVAWFLFRPKADTPSADLSGGDTPVAAPAADPIEKA
                     KALFKEGKAEEALALLTAIPSTDARHDQALVLIDTIKSSAQPGPSTGPAVSDAEVEAA
                     RLAGFASLQAGRYIDAVKSLDVVVRARQDDTEAAQALVKARDAVSALGSAIRSYNEQD
                     YESAQRLLWDLRKQDPKNQDVEEYLFKSYFNDGIMGLQAGSMKKAAESFREAAALRPA
                     DADTQRHLKFARKYPNGPSDLLARIYVKHASPRP"
     gene            20752..21384
                     /locus_tag="PLP50_09845"
     CDS             20752..21384
                     /locus_tag="PLP50_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPA51893.1"
                     /translation="MTEKKHDPLAEHPLGGQPAPAPRGRGKTLSLFPDVSEAPLGPEP
                     ATAEAAAFPPAESTAPAPDEVDEPGPMVDEADVTVTFARRFAAGLADVVILGLFGALE
                     LAAGAILLDLRFPPSALLGVGAFLLLAALILLVLVPLVWGATPGMALADLRVTADDGG
                     SPTLSAATLRFVGALLTGALAGVPLLVAAFDRKGRTLSDLLSRTTLVAAR"
     gene            21418..>22342
                     /locus_tag="PLP50_09850"
     CDS             21418..>22342
                     /locus_tag="PLP50_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPA51894.1"
                     /translation="MRIALGQMNATVGDFSGNGAKIRALWKEAEAAKADLLLVPEMAL
                     CGYPARDLLERPAFLSEAARLLDDLVRGSGRTALLAGTVLPNPAKTGKPLFNAAVLLR
                     NGRIAAIARKSLLPAYDVFDEVRHFEPARRPTLARLAGRKVGLTICEDLWNDKDFWRA
                     RRLYPTDPGEALVARGADLILNVAASPFSEGKPRLRRRMLARLARDGGLPVVYLNLVG
                     GNDELVFDGGSMVLDGAGRVRARAALFEEDFVVVDVDGPRVAPVSGSEPADLPDDAGG
                     EPLESLRRALVLGIRDYARKCGFRTAVVGLSG"
BASE COUNT         2903 a         8246 c         8091 g         3102 t
ORIGIN      
        1 ggggaggcgg cgcagccgcc gcggggaggc tcgtggcgag cctccccgat ccacttcagc
       61 tctcgtcggc ggacggtccg tagagcgcgg ggaccgggac gtcgcgcagc cggaggtaga
      121 ccgtcagctg cgcgcggtgg tggatcaggt ggttcatcac gaaccccctc acgacggcgc
      181 tccggggcat cgtgaaccag gccttcccgt tctcgaggag ggaccagggc tcggcgaagg
      241 cggggtcggc cgtcgcctcg agggccgcgc gcgcggcctt caccccttcc aggaagagcg
      301 cgagcgcctc gcgcgccgac gcgggccggg aaaactcggc cgggaccccg cccggggcca
      361 tgtcgaacga gggggcgttc agcgtcccgg tcagccactc cggaatcgtc gcgacgtggc
      421 cggcgagctc gcccatcgag aacgacttcg cgtgcggctt gaacgcccag tcctcgtcct
      481 tcacgcgctc gaggaccttt cgtgtgccgg ccatctcgag gtcgaactcg ggaaggagcg
      541 acgcggcgat gctcttcgga tcggtgctca tcaggggacc tccttgatcg gatggcgcga
      601 gtaaagcgta agtctggccg gagtgcaagg ggaaagttcg acccgtcgtc gcggatcgcg
      661 ggaacgcacg atcgcggaag ggtttagcct tccatcgaca tgccccggat cgtggtcgtc
      721 gggtcgatcg ccatcgacga ggtcgtcgcc ttgcggcggc cgctcgccgc cggcctccac
      781 ctcgacggga aggagcatgg acggcgcgtc ggcggcggcg ccgcgaacac ggcgatcccc
      841 ctcgcgttcg ccgggcacga cgtcgtcgtc gtctccgccg ccgggaccga cgcggacggc
      901 gggacgatcc tccgccgtct cgccgaggcg ggagtcgaca cctcgcaggt cgcgcggctc
      961 cccgggccgt cgacccggtc gctcgtcctc gtcgacccgg ccggggagcg gaccgtcgtg
     1021 aacctccatc gctgccgcga gggcggtccg ctgcgccggc tccggaccgt tccggccgac
     1081 gcgatctacg tgaggagtcg cgagctggac ctcgcccgga tcctcgccga gcggctcgat
     1141 tcggcgctcg tcgtcgccca cgtcccgccc gtcgtggccg gagcgcgccc ggcccacgtt
     1201 ctcgtcgcct ccgcgtcgga cctctcgccg gaggaggggg agagaccgtg ggacgtgctc
     1261 cgaagggcgg gcggcgagac ggtccggcac gtcgtcgtca cgcgcggcgc cgcgggagcc
     1321 gaggcccatg cgagcgacgg ccgcctccgg gtcccggctc gcgaggccgc ctccgtcgac
     1381 tcgaccggcg cgggagacgt cttcgccgcg gggctcctcc acgcgctcgt ctcgggcgcc
     1441 ccgatggagg cggcactccg gaccgccgtc gcgtggggga ccgcgtctgt cgaatgcgaa
     1501 ggggtgccgc cgcgcgagcg gatccgcgcc cttctctgaa cgcgctccgt cacgcttccc
     1561 gaacgcggac cctcctgtag ctcgcccggt ggaggtcgcg gacctcgacg ctgatcgctc
     1621 cccgcccctc cgccacgagg cgcgggaagg cccggacgag gacctcgagc gccgcgtccg
     1681 agatcgcctg cttcacgtcg tcggagcgtc cgtccaggat cgcgatcgag agggcgacga
     1741 aggagcggtc cgctgcgccg tcgccgacgg cgtagacgtc gtgcctgacc gcgcggctct
     1801 tcaggtcctt ccgttcgaag ggggccgccg cgaggagcgc ctcatgaagg tcgagaagga
     1861 cgcggcggag gtccggccgg tcggggacgt ttgcggagtg ctcgaggacg aggtgcggca
     1921 tgccgcgatt ctccgacgcg ggcgccgccg ggaggcggcg cccgcgctcc atgccactac
     1981 ttcagcgaag ccatgtcgat gacgaagcgg tacttcacgt ccgacttcag catccgctcg
     2041 tacgcctcgt tcaccttctg gaccgggatc gtctcgatgt cggccgtgag gcccttggcg
     2101 gcgcagaaat cgagcatctc ctgcgtctct ttgatgccgc cgatgagcga gccggcgatc
     2161 gacttccgcc cgaagatgag gttgaagacg gtcggcgacg ggtgcgggta ctccggcgcg
     2221 ccgacgaggc agagcgtgcc gtcgcggcgg aggagcgtcg tgtaggcgtc gaggtcgtgg
     2281 gacgccgcga cggtgtcgag gatgaagtgg aaggtccccg cgtgcgcctt catcgcctcc
     2341 gggtccttcg agaggacgac ctcgtcggcg ccgagccgct tcgcgtcggc cgtcttcccc
     2401 gcggaggtcg tgaagaggac gacgtgcgcc cccatggcgt gggcgagctt gacggccatg
     2461 tggccgaggc cgccgaggcc gacgacgccg accttcttcc cggggccggc gccccagtgg
     2521 cggagcggcg agtaggtcgt gacgccggcg cagaggagcg gcgcggcgcc cgccgggtcg
     2581 agccccgccg ggaccttcag gacgaacgcc tcgtcgacga cgatgacgtt cgtgtagccg
     2641 ccgtaggtca tgccgccgag gtgggcgtcg gggctgttgt aggtgaagat cgcccccttc
     2701 gcgcagaact gctcgaggtc gtccttgcac tccgagcagc tccggcagga gtcgaccagg
     2761 cagccgacgc cggccatgtc gccgaccttg aatcccttca cctccgaccc gacggccgcg
     2821 acgcggccga cgatctcgtg gccggggatg caggggtagg tcgtcccgtg ccactcgttc
     2881 cgggcggtgt ggaggtcgga gtggcagatg ccgcagtaga ggacgtcgat ccgcacgtcc
     2941 ttcgggccgg ggtcgcggcg gtcgatctcc atccgctcga ggggggctgt ggcggcgacg
     3001 tttccgtagc cgagggcctt gatcatcggt cggtctcctt ggggcgcggc cgcgcccggg
     3061 ccgcagcgta tacgacggct ccgggcgttt cgtaaagcca cggcgtcttc ccggcgcctc
     3121 gccgcggagg ccggtcgtcc cgccgggcgc cgggaggacc cctccagcgc gcgcctggcg
     3181 tcgcccgctc acggcgcccg gagaggccgc cctttcacga agacggagtc ctgggactgg
     3241 ttgtcgatga cggtggcgta ggcgaagaag ccgcgaacgc cgtcgccgct gacgagcgcc
     3301 caggccgccg ggacgtcctg cgtgacaccc gcggctccga agacgtcgtt gacctggagc
     3361 ggaaggaacg gcggaacggt ccgcgagagg accgcgagct gggtgccgtc gggccggcgg
     3421 agggagatgg tcacggcgtg cggctccggc gccgggttga agacgccgac gttcgtccgg
     3481 aagccctgcc ccggagcggc gctgtgcgag agggagagga gcgccgcctg cgtcgtcgcc
     3541 tcggccgccg gctggcccgg aatgctcgtc cccgtcgtcg gcgctccccg ctcgggcgtg
     3601 tagacgcggc tctcgacgtg gactctcccc gaaagctcga tcgccccggc cgtctcgggc
     3661 gccgaaaaga gcgtcgcgac gacgtcgtcc aggactctca tctcgcgcgg gccgacctcg
     3721 aagctctttt ccatcacggc cgggcaggcg cccgtgaagc agcggtaggt cgccgtcacg
     3781 gtcgcggccg cgtcgggcga cgggttgaag acgcggacgt cggagtggaa gaaggccggg
     3841 gggacgccgt ggatcgacgc cgccgccggc agcgtgacgc gctccgggtc ggccccgcgg
     3901 gcgtttcgtc ccttgacgaa gaccgagtcc tgcgagcggt tgtcgatgac ggtggcgtag
     3961 gagtagagct ctcgcacgcc gtcggccctg acgacggcgt agacgtcggg cacgtccccg
     4021 gcgatccccg ccgcgccgaa gacgtcgttg acctggacgg ccgagcggcc ggagacgtcg
     4081 cgcttcacct ctcccagctt cgttccgccg ggcgcgtgga gggtgacggt gacggccgcg
     4141 acgtcggggc cggggttgta gacgcccaca ttcgtccgga aggccccgcg ggagagggag
     4201 aggaggaccg actcggcgaa cgcctcgtcc accgggaggc ccgggacgtc cgacccgctc
     4261 gtgggcgcgg ggagcgtcgg ggtgtagacg cggctctccg ccaggacggg gccggtcagc
     4321 tcgatggtgc ccgcggtctc gggcgcgccg aagagggaga cgaccgcgtc ctcgaagacc
     4381 ttcagctcgc gcccgttcac cgtgaagctc ttctccgtgt cggccggaca cgggcccgta
     4441 aagcaacggt agcgcgccgc gacggtgacc gccccggaag tggcggggtt gaggacgcgc
     4501 agatcggagt ggaagaacgc cggcgccacg ccgtggatgg acgcggcggc cgggacggtg
     4561 accgtgtccg ggacggtctc ctgctggatc gcgagcgcct cgtgcacggt ctttccgacg
     4621 agcgagggaa ggtcgcgtgc cgccctctcc cgcgcgagga cggccgccag ctccgggctc
     4681 ccgaggctct ccagctgggc gatgacgccg aggagaccgt cgacctgctc gctcgtgagg
     4741 accgcggtcg cggctccctc ccccgtcacg agcgcgcgca cgtgcggctg ccaggcgaga
     4801 acgttcgcga gcgtcgccga gaggagcgag ggactcgcgg cgacgagggt cgcgagctcc
     4861 ctcgcgtggg cgtagtagag ctccgtggtg cgccggcccg ccgcgctccg gctgaagacc
     4921 tcgtcgcgca ggcggcggaa gatcgaggtg tcgacggtgc tctcggccag cgagcggagg
     4981 aggcgcgcgc cgagcccggc tcccggaggt tgcaggccct cggcgagccg actcgtcgtc
     5041 gcctcgcagg cgaggtcgac gaagaggcag gccgtacccg cgcgctcgaa gcagttctcc
     5101 accatctggc gatcgaacgc ctcgttgttc gggaggtgga cgtcgaccga cgccgtggag
     5161 acgcgggccg acaggtcggc ggagcgatcg ttgttccggc cgaggtccgt ggagacgacg
     5221 aacttccgtt cctcgccggg cgcgacggcg cccagcccgc aggccggtga gaacggagag
     5281 ccctggaggc acgcgggctc gaccttcagg acggtcgatt cgctccagag cgtgaggctc
     5341 gcgtcgttcg ccgtggcgcc tccccggttc cggacggtca cgtcccagac cggcgggtcg
     5401 ttgacgggcc gctcccggtg cgcaagcgtc acctcgaggt cgactccgtc gtcccgcatg
     5461 tagaagacct tgacgttcaa gctctgatag ccgttctcgc cgctcgtcag ccgctgtctg
     5521 aacgccgagt gcacgttgta ctggacggcg aagcgcgatg ccccgccgat gctgcctcgc
     5581 cactccgggc acgcgatcgc ggcctgaggc gtgatcgtcg accccgcggg gtacccgtcg
     5641 aacggcgagt cccgatagtc acggcaggcc gggaccggct cgagctgcga gacgaggaga
     5701 tacgcgtcct tctcgtacgg gctcgggagg ctcgcccagg accacgcgac cgagacggtg
     5761 gccggctggt cgaaggccca ctggtcgctg gtccccggct ttcgcgcgac cgtctgcggg
     5821 aagctgaacg cggccgttcc gtcatgaacg ccgaagcact gccagggacc gagggaaacc
     5881 accaccgacc tcgcgtcggg ctggatcggg ctcgtcgacc cggtcggcgg gaccccgggg
     5941 ccgcaggacc ggttatgggt gacttcgccc ccgtcgaaac ggtagacgtc ctgtgcgagg
     6001 ccgggtcctg ccgacgcgac gagcgagagc gcgattccgg cgtggaggag ggcgcgacgg
     6061 gagagcgggt gtgacatcgc ggatcctccg tccgatgccc gagggccagc cctgaagatg
     6121 agaggcgatc tcgatttctg atcggatcat aggccgtttg gtccgcgacg cgaatccggg
     6181 ccgtcgacga gtcggtccca cggtccgcga gctctcctgc ggcgtgcggg cgggaggccg
     6241 gcccgccctt cggcacgcac ggggtctgtc ggcgacggcg tgagactcgg cttctcgtcg
     6301 tcgcctgcga cgaccggagc gccggcccct cagcggaagt agccggagac gtcgaggacg
     6361 gcgtgcgcgg gctgggcgga gtcgttccgt accgtcagcc ggccgagggc gcccggcgag
     6421 gcgatcgcgc tcgaggcacg cgtcttcccg ggggcgaagc cgaggacgtg ggccgtcggc
     6481 gtcggcccgc ccgcgcccca gagcctcaaa acgcctccgg cctgtggggc gacgacggtg
     6541 aggttcaccg cgagggcgcg cgcgtcggcc gggacgccgc acgtccccgc cgcgtcgaac
     6601 gagagagagc cgttcgcggc gacgggcgtc gcggcgccga ggcgcgtgtc gaggacccgg
     6661 cacggcgtga cggtgtggaa ggccgtcggc gtctcctccc cctcgaacgc cccgacgtcg
     6721 cagcggactc cggccgggcg cgcggcgccg cgcgcgtcga cggcggaggc gcagcccgcc
     6781 caggccgcgt cgatcgccgg gcttccgggc aggagggaga ccgtctccac gagcccgccg
     6841 tgatcggcga gcgggccgag gagcggctcg acgacgacgg gcgcggcgac gcagttctcg
     6901 tcgttgccgt cgcccaggtt tcgctccggg tactcgatca cgtcgccgcc gttggtcatc
     6961 cggggcgacc cgacgtcgca gttcttcccg atgttccagg ggttgccgcc gttggcggcg
     7021 gtgttgtgcg ccacgatcga ggcgcggagc gtgactctcg aggccggggc ggcgacggtg
     7081 atccccccgc ccgtccagcc ggcgtggttg tgcgcgaccg tgaggtgaga gagcgtgacg
     7141 ttcgggccgg aggggtagat cgagaacccg ccgccgatcg catagcccga cggcgcgatg
     7201 gccccttcgg cgcggttccc ggtgaaggtg acgttcgtga ccgtcgcgct ggtcgggtgg
     7261 tagagccaga ggccgccccc ctgcgtgaag gcccggttcc ccgcgacgag cgacccggtg
     7321 aaggcgaacg cgccgtcctg gtggtagatc gcgccgccgt tgccgccggt cgcgcggttc
     7381 tcgaggaagg tcgagtcgga gatgacgaga gcctgagggc tcaggtacat gacggagtgg
     7441 atcgcgccgc cggcccaggc gacgttgtcg cggaaggtgc agcgctcgat ccgcacctcc
     7501 ccgtcccccg cgccgtcgac gtagagggcg cccccggtcg ccccgaagct cgagacgcgg
     7561 gcctgcgtct cgttgccgac gaaggtcgag tctcgaatcg tcaggccgtt cgcggggccg
     7621 gtcccgtcgc cgccgaggta gccgatggcg ccgccgttgc ctcctcggtt ccggaggaag
     7681 gtgctgccgt cgaccgtcga gacgcctccc tggatgtaga gggcgcctcc gcccacgtcg
     7741 tcgcccgcct gggtgcagac gttgtcctcg aagcggacgt tctcgatcgt gagggccgcg
     7801 gtcccccgtt cgtggtagtg gctcctcacg gcggcgccga ggtcggcgac gcgcccgcgc
     7861 cggagcgtga ggttgcggag cgccagctgc cgctggtagt cgacgtagaa gaggcggacc
     7921 gcgtcctgcc cgtcgagcgt cacgaggccg ccgccgtcga ccgaggtggg cgtcggggac
     7981 gagatcgtct tcgtcgccgt gaccgggatc gtgacggccc cgcccccgca cgagaaggtc
     8041 acggcgcccc cgcccgcgag cgccgcggcc agcgcggctt cggtgcacga cgccggcgtg
     8101 ccgctcccga cgacgccggc ggccagcgcg gggccgccga agaggaggaa cgcgagatgg
     8161 agcgcgcgac gcacccggga acgatccctc ggcaggaggc cggcggcaat cctcctcggg
     8221 tctagaaagc cctcgccgga cgacgagcgg cggtgcctcg ccccttcccg cgcctctgct
     8281 accgtcgcgc catgccgccg ctcgtcgccg cgcagcgcct gtcgaaggcg ttcggcgccc
     8341 gggacctctt ctcctccctc tcgttcggga tcgagccggg cgagcggatc ggcctgatcg
     8401 gcccgaacgg ggcggggaag tcgacgctcc tctcgatcct cgccggggag acgacgcccg
     8461 acgacggcgc ggttgtcttc cagggggggg tctccgtcgg cctcgtcccc cagatcccga
     8521 gcttcgcgga aggggcgacc gtccgctcgg tcgtcctcga gggggcgctc gcacacgtct
     8581 cgcatggcga ggaggtcgcc tgggaggcgg agctgaagac cgacgaggcg ctcgcgaagc
     8641 tctcgctcac gtcggggggc ctcggccccg acagcctcgt cgcgacgctc tcgggcgggc
     8701 agaggaagcg cgtcgccctc gcccgcgagc tcgtccgcca gcccgacctc ctcctcctcg
     8761 acgagccgac gaaccacctc gacgtcgaga gcatcgtctg gctcgaggag ctgctttcgc
     8821 gcgcgccgtt cgccacggtg acggtgacgc acgaccggct cttcctgacg cgcgtggcga
     8881 cacgcatcct cgagctcgac cggaggaacc ccggcggcct cctctccgtc cccggcggct
     8941 tcccgaagtg gctcgaggcg aaggagacgc tcctcgccgc gcaggagagc cgggaggcgt
     9001 ctctccgcaa tgtcctccgc cgcgagaccg agtggctccg ccggggaccg aaggcgcggg
     9061 cgacgaagca aaaggcccga atcgagcggg ccgaggcgct cggggaggag atcgagacgc
     9121 tcgaggagcg gaacgtctcc cggaccgcga cgctcgattt cgcgggcgcc gggcgcgccc
     9181 cgaagcgcct cctcgtcgcc gagggggtct cgaaggccta cggcgggcgg actctcttcg
     9241 cgggcctcga cctcctcctc ggccccggca gccgcgtcgg cctgatcgga ccgaacggct
     9301 gcgggaagtc gacgctcctg cgcgtcctcc tcggggccga gcgccccgac gccggcaagg
     9361 tgacgcgcgc cgacggcctc tctgtcgccc tcttcgagca agggcgcgag tcgctcgacc
     9421 ccgacgcgac ggtgaaggag acgatctgcc cggaggggga ccaggtcgag taccgcggcc
     9481 gcttcctcca cttccggagc tggctcgacc gattcctctt ctcgcaggag caggccgaga
     9541 tgaaggtctc gcgcctctcc ggcggcgagc aggcccggct gctgatggcg cgcctgatgc
     9601 tgcggcccgc gcagctcctc gtcctcgacg agccgacgaa cgacctcgac ctcgcgacgt
     9661 tgaacgtcct cgaggaggcg ctcgccgagt tccccggcgc gctcctcctc gtcacgcacg
     9721 accgcgcctt cctcgaccgc gccaccgact cgctcctcgc gttcgatccc gagggaggcg
     9781 gcggcgtcac gacgctcgcg ggcctcgacc agtgggagcg gtggcggaag gagcggcgcg
     9841 agtcgacggc gaaggcctcc ccgaagcccg ccccggcggc gccgccaaag ccggcgaagc
     9901 ggcgcctcgg ctacctcgag cagcgggagc tggacgggat ggaggcgcgg atcctcgagg
     9961 cggaggagcg gctcgccgcg cggaaggccg agtgcgagag accggacgtc gtctccgacg
    10021 gcccgcgcct cgtcgcgctc gcccgcgaga tcgaggaggc gcaggccgag gtcgaccggc
    10081 tctacgcgcg ctgggccgag ctgtccgcct gaccggcctc ccggcacccg cgccgatctg
    10141 ctagcgtccc cgccggtgca ggagccgccc cggtccgacc cggccgtccg cccggcgggc
    10201 gggacgccgc cgacccgcct gccggcgctc cggcgggagg ccctcgtcac gactctcctc
    10261 gcgctcgtct gggccgcggc ctggtcgctc gggcggatcc aggagtacgc gccgcacgcg
    10321 agcctctggt tcccgccggc cggcctcacg ttcgcggcgc tcgccgtcct cgggccgcgc
    10381 gccgtccccg gcgtggcggt cgcggcgatc ctcgtcacgc tccggctcgg cgaggtctac
    10441 gacgtcgact acgacctcga ggccctcctc gtcgcgggag cgctcttcgc cgcggcccac
    10501 gtcggcgcct tcggcctcgg ggcggcggcg ctccggcgct ggtccggaaa cagcgtgccg
    10561 caggcggtca ccgccttcct cgtggtcgcg ccggcctcgt ccgcgctcgc ggccgttggc
    10621 ggcgccgtgg cgctcgccac ggcgggggtc atcgagcgcg cggaggcacg cgcgatcctc
    10681 ctcccctggt tcgtcgggga cttcgtcggc gtcgtcgccc tcggccccgc cctcgccgcg
    10741 gtcctcgagc gacttgccgc cacgttccgt ctgcgctcaa ctcccttctt cggggcggtc
    10801 tcccggcttc gcccggcaca cccgggatgg caccgattcg cgctcctgtt cgccctctgc
    10861 gtcctccccc tcgccggatc gctcgtcctg cttggaggct tcggcctccg cggcctggcc
    10921 accgcgttcc tcgtcttctt cgcgatcgtc ccgctcatgt ggatcgtcca cacggagggg
    10981 tcgaaccgcg cgttcggcgc cgtcgccgcg ctgagcgtcg cgatcgccgg cgccggcgcg
    11041 ctcgccggcg ccggcgatca cacgacggcg taccagttcg cgatgatcgt cctggcgggc
    11101 gccgcctact tcgggctcgc cgtgccgtcc ctctacctcg acaacgagca gctccggcgg
    11161 ctcgccacga ccgacccgct gaccggcgcc gcgaaccgcc tcgcgttcga cgaggccgcg
    11221 atccgcgaga cggagcgcgc gcgccggttc gggatgccgc tctccctcgt cctcctcgac
    11281 ctcgaccgct tcaaggcggt gaacgacacc tgggggcacg gggtcggcga cgccgttctc
    11341 gtggaggtcg tccgccgtct ccgcgcggag ctgcgggaga tcgacgtcct ggcccgcgtc
    11401 ggaggcgagg agctggccgt tctgctgccg atgacgccgt ctcccgccgc cgccgacacc
    11461 gcccgccggc tcgcggcggc gctcagggcg cggcccgtga cgcggggggc gatcgcggtg
    11521 ccggtcacgg ccagcttcgg cgtcgcccag gtcgacccgg cggctggctt cgaggcggcc
    11581 cgcgatcgag ccgaccgcgc gctgtacgat gcgaagcgcc tgggtcggga ccgggtcgag
    11641 accgccggcg gaagctgaag ggagcgggat gcagcacctc ttcgaggtcg tcaacgacct
    11701 cttcgcgttc ttcgtgccgg tctccgacct cctctgggac ttcccgaaga acgtccccgc
    11761 gtgggcggcg atcccggtcc tcggcgagtt ctcgttcgcg atgctcctcc tcctcgggac
    11821 ggggctcttc ttcacgctcc ggacgggcgc ggtccagctc cggcgcttcg gcgagagcat
    11881 ccggatcgtc atggaccgga aggcgtcgga gacgggcatc agtcccttcg ccgccttcat
    11941 gctcagctcg gcgatgcgcg ccgggccggg gaacatcatg ggcgtgacgg gggcggtgac
    12001 ggtcggcggc cccggggcgc tcttctggat gtgggtcgcc gggacgttcg ggatggcgac
    12061 cggcttcgcc gaggcgacgc tcgcgcagct cttcaaggag cgcaaggaga gcgagttcgt
    12121 cggcggactg ccgttctacg ggatgcggat cctcgggaac cgccgctggg tcggcgtcac
    12181 gctcgcgctc cttttcatca cgtacgccct cctgaacgtc ccgagccaga cgttccatct
    12241 cttcacggcg ctcggctccg tcgcgtcgac gctcgccggg acgacctatg gacggacctc
    12301 gggcgtcttc tacgcgatcg gcgcgctcct cgtcgtctcc gtcgccgcgc tcgtcctcgg
    12361 cgggctcaag cgcgtcatcc gcttcaccga cctctgcgtc ccgttcatgg cggtgctcta
    12421 ctgcggcgtc gtcctcctcc tcgtcgtcct gaactggcgg ctcgtcccct ggttcttcgg
    12481 ctcggtcttc gcggaggcgt tctcgcccga ggcgatcttc ggaggcagct tcggcgtcgc
    12541 gctccagcag gggatcaagc gcggtctcat ggtgaacgag gcaggacaag ggacgatcac
    12601 gatggccgcg gccgccgccg acaaccggca ccccgtcgag caggggctcg tccagtcgat
    12661 gggggtctgg ttcgacacgg tcgtaatctg ctcgatggcc ggcttcgtca ccgtgatggc
    12721 gaagctctgg gccgccccgg acgtgttcga ctggggggcg cggaaggccg acaagctcct
    12781 cgtcttcatg tcctccgcgc ggcacctcac gccggggacc ggcttcgacc cggccgtcca
    12841 ggtcgccctc tccgtcagct acgccctctt cgccttcacg acgctcatcg ggatgatcgt
    12901 cttcgccgag atcagcgcga acatgctctc gcggaagccc cgcttcatcc tcgggctgcg
    12961 cgccctcggc gccctcttct tcgtcccgtt tggcgtcctg acggtcctcg ccgacctgca
    13021 gctcgggaac atctggtacg tgaccgacct cgtgaacatc ctcgtcgcct cggcgaacgt
    13081 gccgatcctc ctggccggcg gaaagcacgt cgtcgccgcg ctccgccact acgagcggac
    13141 cggcggacgg gaaggcttcg tctccgagag ggacatcggc ctcgcgacgc cgtactggac
    13201 ggaggaggcg cggaagggct gaggcccgcc cggcggcgac gacggcccgg aaccccggga
    13261 ggggcgcttc cgttagagtc ggcatggact tccgatcccg aacgaagaga ggtgtcccga
    13321 tgacccgtct ccgaccgcgt ctcgtgtcgg cgtgcgccct tctcctcctc ctcctcgccg
    13381 cgacccttcc cgccgccgcg gcgaagccgg ccgcgccggc acgggagatc gacgccctgc
    13441 tcgtccgggc gatccccgcc gacggtccgg gcgtcgcggt gatcgcggtg aaggacggga
    13501 agaccgtctt ccggaaggcc cgcgggatgg cgagcctcga gctcggcgtg ccgctctccc
    13561 ccgaatccgt cttccgtctc ggctcgatca cgaagcagtt cactgcggtc gcggtcctga
    13621 tgctcgccga ggaggggaag ctctcgctct cggacccgat cacgaagtac ctccccgggt
    13681 acccgacgca cggccacacg atcaccgtcg agcacctgct gacgcacacg tcggggatcc
    13741 ggagctacac cggcatcccc ggatacatga gcgggccgaa gatccgggcg ggcctgacga
    13801 cggcggagct cgtcgaggtc ttctcgaagg agccgatgga cttcgccccg ggggcggagt
    13861 ggcgctacaa caactccggc tacgtcctcc tcggcgcgat cctcgagaag gtctcgggca
    13921 agccgtacgg cgagctcctc gccgagcgga tcttccggcc gctcgggatg aagtcgacgc
    13981 gggtcggcga cgaggggccg atcctcgcga agagggccgc cggctacacg cgcgacggcg
    14041 agaaggtcgc gaacgcccgc ttcctgagca tgacgcagcc ccacgccgcc ggcgcgctcg
    14101 tctccaccgt cgacgacctc gcgctctggg acgccgccct ctacggcgag aagctcgtga
    14161 agcgggcctc gctcgtgcag gcgtggaccc cggccgtcac ccgcgacggg aggccgacgc
    14221 gctacggcta cggcttcggc gtctcgacgc ttcgcggcgc ccgctcgatc gagcatggcg
    14281 gcgggatctt cggcttctcc acgtacgccg tgaggctccc ggacgagaag gtctacgtcg
    14341 ccgtcctcgc gaacagcgac gcccccgcgg ccgacccggg gacgctcggg aaggagatcg
    14401 ccgcgctcct gatcgggaag cccttccccg cgcgcgtccc cgtcccggtg gacccgaagc
    14461 tcctcgagcg ctgggtcggc gtctaccgct tcgacccgca gacgacccgg acgatcaccc
    14521 tcgacggcgg gaagctcttc tcccagcgct cgggcgggcc gcgcctcgag gtgaagccgc
    14581 tctcggagag cgagttcttc ttcgaccgct ccctcacccg gatctcgttc gtcgccggcg
    14641 ccgacgggaa ggcgaaggag gccctcttct accccgaagg ggccgacgag gcggagaagg
    14701 gcgtccgcga gggagacgtc ccggcggttc cgtcggagac gaaggtcgac ccggccctct
    14761 acgacctcta cgcgggggag tacgagctcg ccccgggctt cgtcctgacg gtccgcaggg
    14821 acgggaaccg cctgctgacg cgcgcgacgg ggcaggagga gctcgaggtc ttccccgcct
    14881 cggagacgga gttcttcctg aaggtcgtcg acgcgcggat cacgttcgtg aaggggaccg
    14941 atgggaaggt ggcgagcctc gtcctccgcc agggcggccg cgagatgccg gcgaaacgga
    15001 tccgttgacc ggaccttcca cgcgcgggga ggggcgtgag cacgcgctcc tccccgcgcg
    15061 accccgcgga gcgaccggcg cccgctcctt cgcccacccc ggccggcggc gtcagcgcgc
    15121 cgtcgcgtcc ggaaggaccc gccagagcgt gaggccgggg ttcgcgtacg cccgctcgag
    15181 aagtccgagg cgctcgagct cggagagctt cgcgagcccc tcctcgccgt agaccgcccg
    15241 ctccggcagg ccgtcgacga cccaccggac gcggtggcgc gataggagcc ggcgcacgtc
    15301 cgcgacggtc gtcctcctct cgcccggccc ctctccgggc gcggcgaggc gcgagcggta
    15361 gaaacgctcg acctccgcga tgcgcttctc gacgacggag cccggaacca ccgcgaggtg
    15421 ctggcggacg tgccagctcc agccgacgac cgccggcagc ccggtgtgga cgctgaaccg
    15481 cgatccccag cggtactcgg ggaggaccgc ctccaggatc gccggcgagc cgcgctcgtt
    15541 cgcgcggagc caggcgaacc ccgccccgtc gtaccggagc gggacgagcc gcccctcgac
    15601 gcagagcgcg gagtcggcga ggaacgctga gccgtcgagg ccgcgcggcc gcccggccgc
    15661 ccccgagccg agggcgtccg gcggggccag cgcgccctcg aacggccggc acgcctcccc
    15721 gggcgtgacg cgcgggaagc gctccgcgaa gcgcgccggc gtcgccgtga gggggtagag
    15781 cgccgacgcg ccgaggagga gcgccgccgt cacgggccag gagccgggac gcgtgcgctc
    15841 gccgtcccgg agcgccacga cgacccacgc gagcgccggt cccgacgcga cggcgaggag
    15901 ggcccacgcc tggaaggaga gcttgaagac gctgttcatc cggtcgccgc cgacggagag
    15961 aacgtcgacg ccgagcgtga ccaggagggc cccggcgccg aggaggagga cggctcggcg
    16021 ctccggggcg gcgccgccgc ccggtgcgag cgccagcgcg gccgccgccg atgcgagcgg
    16081 gaggacgacg atttcggacg ggattcccga ggcgagcccg aatgccaacg cgaggacgag
    16141 gagtcccgca gcggcgagcc acgccctacg ccccgctcct ccctccgacg ccgcatgcct
    16201 tcgggcgacc tcgagcgcca cgaggctcac cgccaggaac gccgggagcc cccagagccc
    16261 gagccacgcc gcgagcggca tcgccgcgcc ggcgttcctc gcgacgctcg tgtagggggc
    16321 gacgcaggac gcgtcgaacg ggcggaagag gagggccgag agggcgagcg ggccgaggac
    16381 gagcgcgacg atcgcgaaga gcccgccgcg cccgagtccc gtcgcccgga acgcgtccgc
    16441 gagcgcgagc gcgcacgcca ggaggaggag gcgcagctgc ccgaggttcc cgagcaggtc
    16501 gagggacgag gcggagagga ggccggcgag aagcggcgcg ccggctcgcc tctcctcccc
    16561 ccgcgcggcg gcgctccgga cgaacgcggc ggggagcgga gaaggaccgg acaccggacg
    16621 gagagtgtcg gctcctcggc ctgctccgag acccgcagcg gatcgccgac ttccacgcga
    16681 tgtgcgacgt cttcgtcctc ccgagccgct ccgagatgat ggcgctggtc caggtcgagg
    16741 cgatgctggc cgggaccccc gtcgtcgcga gcgacatccc gggcgcccgg gtcgtcgggc
    16801 gggagacggg gttcggtctt ctctgtccgc ccgagtcgcc ggaggcgctc gccgcggcgc
    16861 tcgagggcgc cctccggagg cgagaggagc tgcgccccga cccggcgctc gtcgcccggc
    16921 tgttctcgcc cgagacggcg gtctgccgga acgaggagct cctgacggac gtggcgggcg
    16981 cgtccgcgcc cggcaggacc acgtcatcct gagacagggt cccgacattc gggcggtgct
    17041 tgcgggggga gatgccgacg aagaccagga gcggcacggg cagcacgtgg gcatggcttc
    17101 ccgtgatcgc cacgattctg ctcgccacgc tcgttcccgg catcccggag gaacgtggcg
    17161 acggacacgc cgacgttcac cccgaccttc acgccgacgt ccacgccgac ggtcactccg
    17221 acggtgaccc cgacgacgac gatgacgccc gtccccaccg cggccccgga cgtcgacctc
    17281 ggcgcgacga cggccgttcc gggccaggcc ctcctcgcct ccggaagcgt ccccgacacg
    17341 tccctcagct accagctctg cctgatcgca catgccacgt ggacgctcgg gtcgcccttc
    17401 ggcggggtct gcacgctttc ggcgccgctg acgtccccga cgatcgcgtt cagcggagtg
    17461 ccgctcggct ccgctccggg agcgggaagc tatgacctcc tgctcctcgg gcccgcctcc
    17521 acggtcctcg cggccgacga cgcctccgcg gcgcccggcc tcgtcgtcac cgggctcgcg
    17581 aacgtccccg tcggcggacg cgccggcctc ctcggcttcg cgttcctgat cgcggccggg
    17641 gcctgggtcc ttctccgcca gttccgctga ccgcgagccg ccgcgaggcg gcggctcgcc
    17701 ccccgagcgg acggcctccc gtccgcccgg gaacgcgccc gcctactcgc ttccggcgaa
    17761 ggcgacgtcg aggagcttgt tctcgaggac ctgggacgag gcgggggcct cgaggcgcag
    17821 gtagtgctcg gccgcgccga cgagggcggc ctgcggcacc aggccgacga tctcggaacc
    17881 gacgaccggg acgccgaacc gctcggcctc ggagcggacc gtctcgaaga cacggtggag
    17941 cggcgtcttc tcgtagttcg tcatgttgat cgagacctgg acgatgccgc gcgcctcgag
    18001 ccggaccccc atcgccttca cgaaccggaa gcctcccgag aggtggcgga tcgacttcgc
    18061 gatccggtcg gcgatcgaga gatcggccgt tccgaggttg atgttgtagg cgatgagcgg
    18121 ggagcgcgcg ccgacgacgg tcgcgcccgc cgtcgggtgc ggcgcttcgg gtccgaagtc
    18181 gggcttccac tcggggctct tcatcttctc ggcgagcccc tcgaactccc ccttccggat
    18241 cgtcgcgagg ttgcgccgcg cctcgctcgt ggcggcgtcc tcgtagaggt agatcggcag
    18301 gccgtgcttc gccgcgagcc gggcggagag ctccttcgcg agcgcgacgc agtcggccgt
    18361 cgtggcgccg cggatcggga cgaaggggat cacgtcgacg gccccgaggc gtggatgggc
    18421 gccctcgtgc ttcgtcaggt cgacgtggcg gatcgcctcg tcgaccatcg cctccatccc
    18481 gcggaggagc ccgggcccgt cgcccaggaa cgtcaggacg gagcggttgt ggtccgcgtc
    18541 ggaggtccag tcgagcagga ggacccccgg accggccccg acggcggcct tccggacggc
    18601 ctcgacgacc tccggccgcc gtccctcgct gatgttcggg atgcactcga tcaatgtcgt
    18661 catctcaacc tctccccgct cgggggccga agatcatacc gtgcgaccgt cgaggcgctt
    18721 gccgccggaa aggcgccttg ttacgctgac ttcgagaggt tcgcatgccc gagctcgctg
    18781 cctatccagg gagtcccgct ctctccgccg aggcccgcga gaaggtcctc cagacgttcc
    18841 gccacacgct cgagatggct cgggccggcc ggaacgagga cgcgctcctc ggctgcgact
    18901 tcatcctgaa gatggacgcg cgcttcgcgc cggcgcgcca gctcctcgcc tctctccgcg
    18961 ggatcgccgc ggggaccgtc gtcgacctgg ccgagttcgc gccatatctc ggcggggccg
    19021 cgcccgccgc ggcggcgccc gcgcccgtcc cggaggcgcc tccggccacg cccccctccg
    19081 gatacggcgt gccgccgccg cccgtggagg tcctgccggc cgtcgacccg ttcgccgcgc
    19141 ccggcgccgg gctcgacagc ctgagcttcg acgacctcgc ggcgaacccg ttcgctccgg
    19201 cgccggccca cgacccgttc gccgccccgg cgcccgccgc cgacttctcc ttcggagacc
    19261 cctcgccggc cgcggcgccg gccgcggacg aactcgcccc caccgaggcg gcccctgacc
    19321 cgttcgcggg ccccgggccc gccggcgacc cgttcgccgc gcccgcccct gccgcgtttc
    19381 cgtccgcgcc accggcggcc tccggcgtcg acccccggat cgcgcagttc ctccggcagg
    19441 gggacgaggc cgaggcccgg ggagccctcc aggaggcgat cgacctctgg agccgcgtct
    19501 tcctgatcga cctgtcgaac gaagacgcct cccgccggat cgacacggcc cgcgcgcggc
    19561 aggacgaggc cgcccggcag ctcgacgtcc tcctctccga gggggtccag ctctacgagg
    19621 ccgggaacct cgcctcggcg cgagacgcgt tcctgaaggt cctgacgctc tccgagtcgg
    19681 acgcgacggc gcggaactac ctcaacgaga tcgacgtggc gctcgcctct accggcggga
    19741 cggccgcgca cgcgccctcc tacgcgcccg cgcccgtgcc cgccgggccg gtcgaaccgt
    19801 acctccgcga cgagctggag tctccggcgg ggcccgcgtt cgcggacggg cttccctcgc
    19861 tcgacgagac gccgctcggg gaggaacgcc tcgggggagg cgtccccgcg cccgccccac
    19921 gggtcgaggc gcccgccgaa aagggcggga aggtggacgg gcggattctc gtcggcggcg
    19981 cggtgctcct cctactcggc gtcgcggccg tcgcctggtt cctcttccgt ccgaaggcgg
    20041 acaccccctc cgccgacctc tctggcggcg acacgccggt cgcggctccc gccgccgacc
    20101 cgatcgagaa ggcgaaggcc ctcttcaagg aggggaaggc cgaagaggcg cttgcgctcc
    20161 tgacggcgat tccgtcgacc gatgcgcggc acgaccaggc gctcgtcctc atcgacacca
    20221 tcaagtcgtc cgctcagccg ggcccctcca ccggtcccgc cgtcagcgac gccgaggtgg
    20281 aggccgcccg cctcgcgggc ttcgcgagcc tccaggccgg tcgctacatc gacgccgtga
    20341 agtcgctcga cgtcgtcgtg cgcgcgcggc aggacgacac cgaggcggcg caggccctcg
    20401 tgaaggcccg ggacgccgtg tcggcgctcg gctcggcgat ccgctcgtac aacgagcagg
    20461 actacgagag cgcccagcgg ctcctctggg acctccgcaa gcaggacccg aagaaccagg
    20521 acgtggagga gtacctcttc aagagctact tcaacgacgg gatcatgggg ctccaggcgg
    20581 ggagcatgaa gaaggcggcg gagtcgttcc gggaggcggc cgcgctgcgc cccgcggacg
    20641 ccgacacgca gcggcacctg aagttcgccc gtaagtaccc caacgggccg agcgacctcc
    20701 tcgcgcggat ctacgtcaag cacgcctccc cgcgcccctg acggcccgcc gatgaccgag
    20761 aagaagcacg acccgctcgc ggagcacccg ctcgggggac agcccgcgcc cgctccccgt
    20821 ggccgcggga agacgctgag cctcttcccc gacgtctcgg aggctccgct cggcccggag
    20881 cccgcgacgg ccgaggccgc ggcgttcccc cccgccgagt cgacggcgcc cgctccggac
    20941 gaggtcgacg agccgggccc gatggtcgac gaggcggacg tgaccgtgac gttcgcgaga
    21001 cgcttcgcgg ccggcctggc cgacgtcgtc atcctcggcc ttttcggcgc gctcgagctg
    21061 gcggccgggg cgatcctcct cgacctccgc ttcccgccgt ccgcgctcct cggagtgggc
    21121 gccttcctcc tcctggccgc cctcatcctg ctcgtcctcg tccccctcgt gtggggggcg
    21181 actcccggga tggccctcgc cgacctgcgc gtgacggccg acgacggcgg ctcgccgacg
    21241 ctctccgctg cgacgctccg tttcgtcggg gcgctcctga ccggtgccct cgccggcgtc
    21301 ccgctcctcg tcgcggcgtt cgaccggaag gggcgcacgc tctccgacct cctctcgcgc
    21361 accaccctcg tcgcggcccg ctgagggcgg gccgcggcga gggttacgat ccgcccgatg
    21421 cgcatcgccc tcggccagat gaacgcgacg gtcggcgact tctccggcaa cggggcgaag
    21481 atccgggcgc tctggaagga ggctgaggcg gcgaaggccg acctcctcct cgtccccgag
    21541 atggcgctct gcggctaccc ggcgcgcgac ctcctcgagc gccccgcgtt cctctcggaa
    21601 gcggcgcggc tcctcgacga cctcgtccgc ggatccgggc ggaccgcgct cctcgcgggc
    21661 accgtcctcc cgaatccggc gaagaccggc aagccgctct tcaacgcggc cgtcctcctg
    21721 cgaaacggcc ggatcgccgc gatcgctcga aagagcctcc tccccgccta cgacgtcttc
    21781 gacgaggtcc gccacttcga gccggcgcgc cgcccgacgc tcgcccggct cgcgggccgg
    21841 aaggtggggc tgacgatctg cgaggacctc tggaacgaca aggacttctg gcgcgcgcgt
    21901 cgcctctacc cgaccgaccc gggagaggcg ctcgtggcgc gcggagccga cctgatcctc
    21961 aacgtcgccg cctccccgtt ctcggaaggg aagccgcgcc tcaggcgccg catgctcgcg
    22021 cgcctcgcgc gcgacggagg gctcccggtc gtctacctga acctcgtcgg ggggaacgac
    22081 gagctcgtct tcgacggcgg ctcgatggtc ctcgacgggg ccggccgcgt gcgggcgcgc
    22141 gccgcgctct tcgaggagga cttcgtcgtc gtcgacgtcg acggcccgcg cgtcgccccc
    22201 gtttccggct cggagccggc cgacctcccc gacgacgccg gaggggagcc gctcgagtcg
    22261 ctccggaggg ccctcgtcct cggcattcgc gactacgcgc gcaagtgcgg cttccggacg
    22321 gcggtcgtcg gcctctccgg cg
//
LOCUS       DAOONL010000056        21572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2368_length_21572_cov_19.201329, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000056 DAOONL010000000
VERSION     DAOONL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_21572_cov_19.201329"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..496
                     /locus_tag="PLP50_09855"
     CDS             266..496
                     /locus_tag="PLP50_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51895.1"
                     /translation="MPIASKLLAAAALALAAILLALPADAYLDPGTGSIVVQGVIAAI
                     AAGAVALKLYWKRLKSVFGRRPAAGPGETREE"
     gene            493..>1235
                     /locus_tag="PLP50_09860"
     CDS             493..>1235
                     /locus_tag="PLP50_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017959092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51896.1"
                     /translation="MTGSSTSAGSFRDPAGSVHVLGRRVFRTISGRASAAFESAEEAG
                     LFSRLVSEGRLLPHRRLDEAEANALGLEAPIVLEHPRLPFVSWPYEWSFPALKAAALF
                     HLDVHLDALGRGFTLSDASAFNVQFQGPRPVFVDTLSFRPYRDGEYWLGHRQFCEQFL
                     NPLLMHSLLGVSHAPWFRGSLEGVPATDLAALLRARHRLSWNVAVHVLLPARFQRAST
                     REGAVRVSSGRKLPLEGLLAMLRGLRSWIR"
     assembly_gap    1236..1335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1336..1515
                     /locus_tag="PLP50_09865"
     CDS             <1336..1515
                     /locus_tag="PLP50_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51897.1"
                     /translation="VEFVPKSDPMVQELLRLREDVFDGYTEEHFLECLGRRAEALDTA
                     RVSVNGRLLVSYRRR"
     gene            1527..>3052
                     /locus_tag="PLP50_09870"
     CDS             1527..>3052
                     /locus_tag="PLP50_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51898.1"
                     /translation="MERPHAHLRPWYALLPSAILVAPVLAFLVDKDYSLVAPESLCWV
                     FGAAAVGGAVGLLALRSPGWLRAVLLSLCLSLFVDVQFAPVDRGILVLAFAAFALGVW
                     LLRANAFPILSAGFAALLLSLVLVPTRPAFQAYSGIDVGARPVREELPPLIVLILDEH
                     AGIEGIPLDIEGGAEVREALSELFGSRGFRLYPRAFSQYSSTTDSLSNLLNHSARGTR
                     DVWVREFRGRFSLRESRFLRHAVSSGYAIRIYQSDYLDFCDVEGVRPEACLTYAAARL
                     GSILEQDITWSERAAAIGTSLLSRSALLEITNRTRVWLRRHGVPIPLPGDEPRTLVGP
                     FESMTVLDRIEADVTRGARGRLFLAHLLIPHSPYVYGADCRVTPRDATPHWLKAKDVL
                     PPGPAGSRTEQLTRERYRRYFSQVQCLTLRLRSFFDALEAGGALEDALVVVLGDHGPR
                     IRPQRPRPGSLDSARPIDYVDAFSALFVARGPGVEPGSEPAPRSIVRAFAEVLGYAGP
                     P"
     assembly_gap    3053..3152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3418..3867
                     /locus_tag="PLP50_09875"
     CDS             3418..3867
                     /locus_tag="PLP50_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA51899.1"
                     /translation="MTTEKRRQAVLLMAEDNDDDFLLARSALEEARLLNDLRRVRDGV
                     ELLDYLRRRPPFEDPEENPLPALILLDLNMPKIDGREALSQIRNDPALCHIPVVVLTS
                     STAPEDVQQAYRLGVNSYIAKPVTFAGLVASLRILGLYWFELVVRPE"
     gene            3875..5779
                     /locus_tag="PLP50_09880"
     CDS             3875..5779
                     /locus_tag="PLP50_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA51900.1"
                     /translation="MSPDGRPPRILVVDADADEALLVEGYLREGLGAPDLPVRRTQTA
                     AEARAILAEDPPDVLLLDVRAHDTEALGLLEQMRDEGSPTPAIVLTGRSNAEAALHAH
                     RLGAAEVLVKPSLTPASLAATIRFATQLEESRRKEAETEQALRRAERQAEALVASSLD
                     IVSILDREGRILFTSPSTEKLLGHRVETLVTRNVLEVVCPEDTAAVQAALRRCLESPG
                     TTVSVEFGVHRADGAIRHLEALTTNLLDDPGVLGLVVNSRDVTERHRSEDTLRRLEAA
                     VEQSASIIFLTDATGMITFVNPAFTRTFGYTREEAIGASPRLLRSGRHDDAFYADLWA
                     ELAAGNTVKTEIWNRARDGRLVIVRASITPIRDRRSAVTGYLAVQDDITERNVLNERL
                     RLAQKMEALGHLAREVAHELDAVLSGVLSGIDAAASRIPAGSPAEPLLAEARAAAVRG
                     AALGRELLSFGRGAPMELARVGANALLRDAAAQLRGRIGHGISLHLDLSPEAVAVTVD
                     RRELAYALVQLADFLLESLPPGRTLTLGTTVGPLSEPGLEALALGSGKSWVILTVGDD
                     GTPMDEETRSRLFDPFGSSRALGRGGGLGLAAPFGTVKQAGGFLFAVPAERGNLFRVY
                     LPPAPGSILS"
     gene            complement(5792..9991)
                     /locus_tag="PLP50_09885"
     CDS             complement(5792..9991)
                     /locus_tag="PLP50_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA51901.1"
                     /translation="MRRTALAAAAGYGALGVLWVVFSDDVVRAIVDPERMTTLQSAKG
                     ILFVALSAVFVFAIAVRSAQRFGAEVDLERGERLTAGAWAPVAVFVVLLAAVAGAGWV
                     LHERAIAEKRATTLATLSATAEVKIAQASGWLDALRIDARLLAGEAEVEAVLSAGAGP
                     DSEGSRRDLGAELSRYAEHHPGRSVALLDAAGEVVEQVGPPIRLGPRLKEALESTRTT
                     PEALLAEPGTEEGLPLVIRAVAAVSAAVPGGNVPGWVVVGAPAREQLRALLARAPGAS
                     PSEEAVVVRREGGEAVVVAAETGDTLRTRRVSAVGDSLAARAARGEDGAREEVDDRGV
                     ASFGALRRVSGTDWTLLVRRDAEEAYRPLRRRLAGIALFNLALLAAAAGATLSWWRGL
                     RVRHAFRERELAAERDAVARHFAWLSEASNDLVLLTDESFRIVEANRKAFDTYGYSRK
                     ELIGADTTILRPAADRPAAREAFARLLADGSIHLETIHQRKDGSLFPVDFSGRRIEVD
                     GRPFVQYVIRDITERKESDERIHRLNQLYELLSRANAAIVRTPESVRLARRVCEIAVE
                     VADFRLVWVSEATADGELRALAVAGPAAAIIGEAALTLDPEAPSGHGPTVDAIREGHP
                     VVANDYLSDASTTVWHEAARRFGLESLAAFPIPGAAGPRGALAVGAHRKGYFDDEIVR
                     LLAGLAEDLGFALAARDREVERQRAEEALKVTARRLSDAETIGRTGSFYGDLETQQLS
                     WSDGHYRLFGVTPETFTPSNESFLERVHPDDRERMRALAAANWDGQAAGGHEYRVLDP
                     SGEVRWLNATSELVRDDSGRPVGVFGVVRDVTETKLAEERQRLWSHVLEGSADGVLLA
                     GADQRILLVNRAFETMSGYAAEEVLGRTPRFLGSGHHDRAFYRQMWATIARTGRWRGE
                     IHNRRSNGEVYPVLLSITAVKDERGATSHYVALYTDLTERVAAIERIEFLSQRDPLTH
                     FVNRPGLETQLARAIASSRLEGHALALVALDVDRFSAINESLGGPVGDEILRSIASRL
                     AGAVRTRDVLARIGPDEFAVAFVSVKGTAGCEIAARKVQAIFESPFEVAGRSIPISAS
                     CGVAVSPEDGEDAPTLMRNADAALRHAKSGEGGTLEYFRREMTARAMETLEIETRLRQ
                     ALAKGELLLHYQPQVEVETGRIVGAEALVRWKDPVRGLIPPGVFIPVAEERGLIVPLG
                     EWVLRAAATQLRQWLDAGLPRIVVAVNVSAAQFRSPDFETRLVEVLEASGVPPELIEL
                     ELTESVLARDVEAAAALLGRLRARGVSIAIDDFGTGYSSLGYLRKFPLQRLKIDQGFV
                     REIWNDPSAAAIVRTVIGMSRGLGLKVVAEGVEEKRQFSFLRFHGCDEAQGYFIKRPV
                     PPEAFREFLEAGDFDPDPPAA"
     gene            complement(10128..10934)
                     /locus_tag="PLP50_09890"
     CDS             complement(10128..10934)
                     /locus_tag="PLP50_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51902.1"
                     /translation="MRGRIGLWLLALLLTLGSAVFQRMTGPTHPVRGAVTLGGEEVRF
                     RLLRSHGGEGDQPIVVRVADRAVTGSVAWRRYPTRDAFTMLPMVRDGETLRAALPHQP
                     PAGKLEYQVRLARDGETALFPERPAITRFKGAVSPVVLVPHIVAMFGGMLVANAAGLL
                     ALRGAKRLLGISVVALVLLALGGLFLGPLVQKAAFGAYWTGWPFGTDLTDNKTAFAVL
                     AWAVAAFRARSGRDARLAVGIAALVTLAVFLVPHSAWGSQIDWSKLQAGS"
     gene            10976..11869
                     /locus_tag="PLP50_09895"
     CDS             10976..11869
                     /locus_tag="PLP50_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA51903.1"
                     /translation="MRSRVAPGAVAARLALFGLLLVPAATSRAAGEAVPPVVSGGWLE
                     PKLASAEIVLLDARPLRDFLASHLPGAQSVAVENLRATRGGVPATLHPPELLGTIFAG
                     AGVTPRAHVVVYGAESDVDATYVATAARVAGATRVSVLDGGFARWSADLRPVRVERTR
                     FPVPVARLRGERRLVIDLEEVAKRVGDGRTVLLDVRQAAQWAKERIPGAKNRPWKADV
                     SDGLFRPADRLRADYEAAGVSWEKPVVVYCNSGHQASEAFWVLKYVLGHPDVRLYQGS
                     WLEWSMTPGTPREGDRPAEER"
     gene            11866..12639
                     /locus_tag="PLP50_09900"
     CDS             11866..12639
                     /locus_tag="PLP50_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPA51904.1"
                     /translation="MTPGEIAALLASSFGAGVMNALAGGGTILTFPTLVLLGIPAIQA
                     NATSTVALLPGAAASMAGYRREVATHAAWLKTLFLPSLVGGAIGSVLLLKTPEKLFAS
                     LAPFLVLFATLLFLLQIALAKRKGDAEVHAHDGPTTARWGVATLAQLAVAIYGGYFGA
                     GIGILMLVVLGFLGLTDIHAMNGLKNFFGITINLVAAAWFIWNGAVLWPIALVMVAGA
                     TVGGYAGARFARRIGKEKARAAVVVIGLGVTVVLLLQRA"
     gene            complement(12608..>15172)
                     /locus_tag="PLP50_09905"
     CDS             complement(12608..>15172)
                     /locus_tag="PLP50_09905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA51905.1"
                     /translation="RGVLGTGGSAVVYEAFDRELKRRVALKVLRTDRTSEAALKRFRR
                     EVAIARDAASPRLVRVFDIGQAGDTVFLTMELVEGESLRELLARGPLPPERAVAIAAE
                     VLRALADLHALGIVHRDVKPGNILLSDAGEVKLADFGLARHWEGSETRVTETEALVGT
                     VEYLSPEQALGDLLDARSDLYAVGVVLFEMLAGKVPLRGESAIGTIVAHIRQEPVDVR
                     KLRPEAPAWLSALVGRLLAKDRERRYATATEVLADLEARRARRPPRRLGRALALGGSA
                     ALVALLLTLAFVPVFPWNRPRLTRLAPDGAGGVQALDGKGRVLWRRAGTAASRQIALA
                     RLSGGETGAIAVPGGTDDPRKRVVVLDGLTGRPLADMELPDPAGDRDGAGAFPGFSST
                     FAVGAVFPFDVDGDGDDEVAVSLAHLPLWAGATYVCDPGRRRVVAAFYGSGHHYARAA
                     VDVDGDGRKELILAGLNNRMGYSAAVAALRIPGEGEGRRAWSEVPFAESPDRPAWRDE
                     KSLLAWYALVPDAAFDRDEGQVLEASAGFLRVGAWGPRQVELALDGFPRGPREPGEAK
                     RRSDAREAAYGLLREGMRLRGAGLAREALVAVERALEKARAAGDAPLGQWIARARARA
                     LLDARQPGEADAAWGASFERSAEPARIALEAGRAFHLRGDLARAVAWYRRGLTGRGDH
                     ARIGIYPRDLMLGVVLALGEAGRWDDAAAEVDRFEAAYPDRVRDVAAARAFVAWRSGR
                     RPARYADSVADIGLFRYWGLEARRAGGEAADALLVDVERVGREALDVAPLFVSLRAEL
                     LAALGRRDEARVLATEALDGARDLAATDTGVRAHLSLVAERARKLGFRPAGGGAPQ"
     assembly_gap    15173..15272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15273..15871)
                     /locus_tag="PLP50_09910"
     CDS             complement(<15273..15871)
                     /locus_tag="PLP50_09910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA51906.1"
                     /translation="MSDGSDPSDPYARLSYRRLIAWPARIEREAPFLREVLANAPSRR
                     LLDLGCGTGEHARFLSEEGFEVTGVDASEAQLTQAREAGPGPEFVKGDLAELDAAVTG
                     PFGGAISLGNGLPHLHEKEMASFLGHLARLLLPGAPVLFQLLNYDRILDQGVTSLPLN
                     VRDDGGERVVFLRLMEPRPDGTVLFNPTSLRWRPGAEPPV"
     gene            16155..18482
                     /locus_tag="PLP50_09915"
     CDS             16155..18482
                     /locus_tag="PLP50_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPA51907.1"
                     /translation="MARKGGGRGGLARASTDAARSLNRESSRRRRCIVAATAAIFGAA
                     GLLAIGYRLLSGPRQAPIILISIDTLRADHLPAYGYTKIPTPAIDELARNGVVFENCY
                     THVPLTLPAHASLLTGLLPPDHGVRNNIGFRVAEGTPTLASELKRVGYATGGAISAAV
                     LRRGTGIAQGFDWYDDDLRAEGPEVRTTEVQRDGSETLERALSWLRERANASSGEPPF
                     LFFHIFEPHTPYAPPARYRRAGVSPYDGEVMYADEIVGRLFDELRRLDLFERSLIVLL
                     SDHGEGLGDHGEADHGVLLYREVMRVPFIVKLPGQEGGGRRVNDPVQLVDLNPTLREW
                     VGLPPRPEQRGTSLLATLRGGPAPGDRRVYGESLYGRLHFGWKELFSLTDSRFKLILA
                     PRPELYDVEKDALERHDLLAGGSPGVEASTERDRLHRALRVLIGSGAPKAPNPVDEEE
                     VARLRALGYVGSPTLDPTEDATAPDPKDRIGDLALFRKAMRLKGVGMYRPAAEDLEKV
                     AASSPRMVDAWDELGGIYRRLGQAEKAVDAFRSGLAVAPSRGEAHLALADFLVELGRL
                     DEARREADAAAPYRPGEAEVRRAVIEATAGDPAAARASALRATNALPAAAPFVDGLLA
                     HAREEYATAVPLLEKALDAVKGRSPRTLPRVHYLLGSALVEQSVHVSDEASRTRLRQE
                     AERHLRTELALEPTSAGAVTALCRVHALAGAPEKLRRDLEAFARGNPSPAVFRLAETI
                     FGQAGLKDDAARWGLRARASAGRSRPALPAIAPRP"
     gene            complement(18594..19388)
                     /locus_tag="PLP50_09920"
     CDS             complement(18594..19388)
                     /locus_tag="PLP50_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPA51908.1"
                     /translation="MSGSKDINIKLRIWRQKGPGTKGGLVSYDVPGVSTDSSFLEMLD
                     LVNERLMEKGDEPIAIDHDCREGICGACSLVINGVPHGPRAATTTCQLHMRSFHEGQT
                     ITVEPWMAKAFPVIKDLIVDRSAYDRIIAAGGFVSVNTGVGVDANAIPVPKENAELAM
                     DAAACIGCGACAAACPNASAMLFVGAKVSHLGLLPQGQPERQERVLKMVEQMDAEGFG
                     GCTNMGECEAACPKEIKLEVIARMNRDYIGAKVTKRPKGADKGGGA"
     gene            complement(19388..21301)
                     /locus_tag="PLP50_09925"
     CDS             complement(19388..21301)
                     /locus_tag="PLP50_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPA51909.1"
                     /translation="MELDGKCPTGPMESRWTRHKAAIKLINPANKRKYSLIMVGSGLA
                     GASGAASLAELGYNVSCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVK
                     GGDFRARESNVYRLAEVSNDIIDQCVAQGVPFGREYGGTLGNRSFGGAQVSRTFYARG
                     QTGQQLLIGAYQALERQIGLGKVKMYPRTEMLDLIVVDGVARGIVVRDMVTGKVSSHL
                     ADAVMLATGGYGNGYFLSTNAKGCNGTAIWRAHRRGAFFANPCYTQIHPTCIPVHGEN
                     QSKLTLMSESLRNDGRIWVPLKKGDTRKPGDIPEAERDYYLERKYPSYGNLAPRDISS
                     RAAKQVCDEGRGVGPGGQGVYLDFAESIGRLGESVIRERYGNLFEMYERITDENPYEV
                     PMRIYPAVHYTMGGLWVDYNLMSNLPGLFVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTIGNYLAQGKPAKIDASHPDVKAAEADVNDRVKKLLSIKGKRTVDSIHKQLGLL
                     MWEKCGMGRNEAGLKEAIARIPEIREEFWKNVFVPGSGDDLNVSLEKAGRLADFLELD
                     ELICRDALAREESCGGHFREEYQTPDGEALRNDEKFAHVAAWEWKGEGSTPERHTEQL
                     QFESVHLATRSYK"
     gene            complement(21313..>21572)
                     /locus_tag="PLP50_09930"
     CDS             complement(21313..>21572)
                     /locus_tag="PLP50_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51910.1"
                     /translation="QFSHTDVYTNVVVGFSNVWVSGFYVLAMLALGFHLFHGGFSLFQ
                     TLGLRTPKYEKPIKLVLAVLCTVIVVVNVSFPIAVLAGLVK"
BASE COUNT         2858 a         7928 c         7486 g         3000 t
ORIGIN      
        1 ggccagatcg agtacctcct gcgggaggcg gtgaaggccc ggcggcgcgg gcgggggcgg
       61 gagggggcga acgccgggcc ggctggcccg tgacgaggcc cgggcctgcg gtcgccgtcc
      121 gggccggtgg gcgagcgctg ccggacccgt gaggccggcc ttgcgctctt cccgcggctg
      181 gcgcggtccc ccggaacgcc cccgcatcgg ctcccgccgc gcgcggggta cgatggatcg
      241 gctccctcgc tcgggaagga ggaccatgcc gatcgcttcc aagcttctcg ccgcagccgc
      301 tctcgcgctg gccgcgatac tcctggcgtt accggccgac gcctacctcg atcccggcac
      361 cgggagcatc gtggtgcagg gcgtgatcgc ggcgatcgcg gccggggccg tggccctcaa
      421 gctctactgg aagcggctga agtccgtctt cggtcgaagg ccggccgccg gaccggggga
      481 gacacgggag gagtgaccgg cagctccacg agcgcggggt cgttccgcga cccggccggc
      541 agcgtccacg tcctcggccg tcgcgtcttc cggacgatct ccgggcgggc atcggccgcc
      601 ttcgagtccg cagaggaggc aggccttttc tcgcggctcg tctcggaagg gcgcctcctc
      661 ccccatcgac ggctcgacga ggccgaggca aacgccctcg gtctcgaggc gccgatcgtc
      721 ctcgagcatc cgcggcttcc cttcgtatcc tggccctacg agtggtcgtt cccggcgctc
      781 aaggccgcgg ccctcttcca tctcgacgtc cacctcgacg cgctcggtcg aggcttcaca
      841 ctgagcgacg cgtccgcttt caacgtgcag ttccagggac cgcgccccgt cttcgtcgac
      901 acgctctcgt tccgtcctta tcgcgacggc gagtactggc tggggcatcg ccagttctgc
      961 gagcagttcc tcaaccccct gctgatgcac tccctgctcg gggtctccca cgctccgtgg
     1021 ttccggggaa gcctcgaggg cgtgccggcg accgacctcg cggcgctgct ccgcgcacgg
     1081 caccggcttt cctggaacgt cgcggtccac gtcctgctgc cggcccggtt ccagcgcgcc
     1141 tcgacccggg agggagcggt ccgggtgtca tccggtcgaa agctccccct ggagggcctc
     1201 ctcgccatgc tgcgggggct gcggagctgg atacgnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnngtcga gttcgtgccc aagtccgacc cgatggtcca ggagctcctc
     1381 cgcctccgcg aggacgtctt cgacggctac acggaagagc atttcctcga gtgtctcggg
     1441 cggcgggccg aggccctcga caccgcccgc gtctccgtga acggacgact gctggtgtcg
     1501 taccgccggc gttgaggacg gaccgcgtgg aacgacctca cgcccatctc cgtccctggt
     1561 acgcgctcct tccctccgcg atcctcgtcg cgcccgtcct cgcctttctg gtcgacaagg
     1621 actacagcct cgtggcgccc gagagcctgt gctgggtgtt cggcgccgct gccgtcggcg
     1681 gcgccgtcgg cctcctggcg ctccggtcgc cggggtggct gagggcggtc cttctgtcgc
     1741 tctgcctgtc gctcttcgtg gacgtccagt tcgctcccgt ggaccggggg atcctcgtgc
     1801 tcgcgttcgc agccttcgcc ctcggcgtct ggcttctgcg ggcgaacgcc ttcccgatcc
     1861 tctcggccgg cttcgccgcg ctgctcctct ccctcgtgct ggtaccgacc cggcccgcgt
     1921 tccaggccta ctccgggatc gacgtgggcg cccgcccggt ccgtgaggag ctgcccccgc
     1981 tgatcgtcct gatcctggac gagcacgcgg gaatcgaggg catccccctc gacatcgaag
     2041 gaggggccga agtccgcgag gccctctccg agctcttcgg gtcccgcggc ttccggctct
     2101 acccccgcgc cttcagccag tactcgagca cgacggactc cctctcgaac ctgctcaacc
     2161 attcggcacg cgggacgcgc gacgtctggg tccgcgagtt ccgaggccgc ttctcgctcc
     2221 gcgagagccg gttccttcgg cacgccgtct cctccgggta tgcgatcagg atctatcagt
     2281 cggactacct cgacttctgc gacgtcgaag gcgtccggcc ggaggcctgc ctcacctacg
     2341 cggccgcccg gctcgggtcg atcctcgagc aggacatcac gtggagcgag agggccgccg
     2401 cgatcgggac cagcctcctc tcgaggtcgg cgctgctcga gatcacgaac cgaacgcggg
     2461 tctggctccg gaggcacgga gtcccgatcc ctctgcccgg cgacgagcct cgaacgctcg
     2521 tgggcccttt cgagtcgatg accgtcctcg accggatcga ggccgacgtc acgcggggag
     2581 cgcgcggccg gctgttcctg gcccacctcc tgatccctca ctctccatac gtctacgggg
     2641 cggactgccg agtgacgccc cgcgacgcga ccccgcactg gctgaaggcg aaggacgtgc
     2701 tgccgccggg acccgccgga tcccgcaccg agcagctgac gcgggaacgc tatcggcgct
     2761 acttctcgca agtgcagtgc ctgacgctgc ggctcaggtc cttcttcgac gcgctcgagg
     2821 ccggtggggc gctcgaggat gccctggtcg tggtcctggg agaccacggc ccgcgcatcc
     2881 gtccgcaaag gcctcggccc gggagcctgg attccgcgag acccatcgac tacgtggatg
     2941 ccttctccgc gctgttcgtc gcgcgaggcc ccggagtcga gcccggctcc gaacccgcgc
     3001 cccgctcgat cgtgcgggca ttcgccgagg tgctcggcta cgccggccca ccnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgccccgc tcgatcgtgc gggcattcgc
     3181 cgaggtgctc ggctacgccg gcccaccgcc ggagacggag gagctgctct tgtcggagaa
     3241 gggagaggcc cggctggttc ccgtccccat gcctccgttc tgagcccggg aacccggatg
     3301 cgcccgcgat cgggggaggt ccgccgggga cgtgacttcc ggggccgaaa ggagacaatg
     3361 gggcgccgcg tgccgccggg cttcgcgccc ccggcccccg gcttcgggag gggtggaatg
     3421 acgaccgaga aacgccggca ggccgtcctt ctgatggcgg aggacaacga cgacgacttc
     3481 ctcctggcgc ggagcgccct cgaggaggcc cgcctgctga acgacctgcg ccgggtgcgc
     3541 gacggcgtcg agctcctcga ctatctccgg aggcggccgc cgttcgagga cccggaggag
     3601 aaccctctcc cggccctcat cctcctcgac ctgaacatgc ccaagatcga cggccgcgaa
     3661 gcgctctcgc agatccggaa cgacccggcg ctctgccaca tcccggtcgt cgtcctgacg
     3721 tcgtccaccg cgccggagga cgtccagcag gcctaccgcc tcggcgtcaa ctcctacatc
     3781 gcgaagccgg tcacctttgc aggactcgtc gcctcgctcc ggatcctcgg cctctactgg
     3841 ttcgagctcg tcgtccggcc ggagtgaacg cccggtgagc cccgacggcc gccccccgcg
     3901 catcctcgtc gtcgacgccg acgccgacga ggcgctcctc gtggagggct acctccgcga
     3961 gggactcggg gcgccggacc tcccggtgcg ccggacgcag acggccgccg aggcacgggc
     4021 tatcctcgcc gaggaccctc ccgacgtcct cctcctcgac gtccgggcgc acgacaccga
     4081 ggcgctcggc ctcctcgagc agatgaggga cgaagggagc ccgaccccgg cgatcgtcct
     4141 gacgggccgc tcgaacgccg aggcggcgct ccacgcccac cggctcgggg cggccgaggt
     4201 cctcgtcaag ccgagcctca cgccggcgag cctcgccgcc acgatccgct tcgcgacgca
     4261 gctcgaggag tcgcggagga aggaggcgga gacggagcag gcgctccgac gggccgagcg
     4321 gcaggccgag gcgctcgtcg cctcctcgct cgacatcgtc tcgatcctcg accgggaggg
     4381 acgcatcctc ttcacgagcc cctcgacgga gaagctcctc ggtcaccgcg tcgagacgct
     4441 cgtcacccgg aacgtcctcg aggtcgtctg ccccgaggac acggcggccg tccaggccgc
     4501 gctccggcgc tgcctcgaga gcccggggac gaccgtctcg gtcgagttcg gcgtccaccg
     4561 tgccgacggc gccatccgac atctcgaggc cctcacgacg aacctcctcg acgatcccgg
     4621 ggtcctcggt ctcgtcgtca actcccgcga cgtcacggag cgccaccgct ccgaggacac
     4681 ccttcggcgc ctcgaggccg ccgtcgagca gtcggccagc atcatcttcc tcacggacgc
     4741 gacggggatg atcaccttcg tcaaccccgc cttcacgagg accttcggct acacgcgaga
     4801 agaggcgatc ggcgccagcc cgcggctcct ccggagcggg cggcacgacg acgcgttcta
     4861 cgccgatctc tgggcggagc tcgcagccgg gaacacggtg aagacggaga tctggaaccg
     4921 ggcccgggac ggacgcctcg tcatcgtccg ggcctcgatc accccgatcc gggaccgccg
     4981 gagcgccgtg accggctacc tcgccgtgca ggacgacatc accgagcgga acgtcctgaa
     5041 cgagcgcctc cgcctcgcgc agaagatgga ggccctcggc cacctcgcac gcgaggtggc
     5101 tcacgagctc gacgccgtcc tcagcggcgt cctctcgggg atcgacgcgg ccgcgagccg
     5161 gatccccgcc gggagccccg ccgagccgct tctggccgag gcgcgggcgg ccgccgtgcg
     5221 tggcgccgcg ctgggacgcg agctcctctc gttcggccgg ggcgcgccga tggagctcgc
     5281 ccgcgtcggg gcgaacgccc tgctgcgcga cgcggccgcg cagctgcgcg ggaggatcgg
     5341 gcacggcatc tcgctgcacc tcgacctctc gcccgaggcc gtcgcggtga cggtcgaccg
     5401 ccgggagctc gcctacgccc tcgtgcagct cgccgacttc ctcctcgagt cgctccctcc
     5461 cggccggacc ctcacgctcg ggacgaccgt ggggccgctc tcggagccgg gcctggaggc
     5521 tctcgctctg ggctccggga agagctgggt gatcctgacc gtcggggacg acgggacgcc
     5581 gatggacgag gagacccgca gccgcctgtt cgacccgttc ggctcgtccc gggcgctcgg
     5641 ccgcggcggc ggcctcggcc tggcggcgcc gttcggaacc gtgaagcagg cgggcggatt
     5701 cctcttcgcc gtgccggcgg agcgcgggaa cctcttccgc gtctacctgc cgcccgcccc
     5761 cgggtcgatc ctctcctgag ccggccgcgc ctcaggccgc gggaggatcg gggtcgaagt
     5821 ctccggcctc gaggaactcc cggaacgcct cgggcgggac cgggcgcttg atgaagtagc
     5881 cctgcgcttc gtcgcagccg tggaaacgga ggaaggagaa ctgcctcttc tcctcgaccc
     5941 cttccgcgac gaccttcagc ccgaggccgc gcgacatccc gatgaccgtc ctcacgatcg
     6001 ccgccgccga ggggtcgttc cagatctcgc ggacgaagcc ctggtcgatc ttcaggcgct
     6061 ggagcgggaa cttcctcagg tagccgagcg aggagtagcc ggtcccgaag tcgtcgatcg
     6121 cgatggagac accgcgcgcg cgcagccgac cgaggagcgc cgccgcggcc tcgacgtcgc
     6181 gcgccaggac ggactcggtc agctcgagct cgatcagctc gggcgggacg ccgctcgcct
     6241 cgaggacctc gacgaggcgg gtctcgaagt cgggcgatcg gaactgggcc gccgagacgt
     6301 tcaccgcgac gacgatccgg ggcagccccg cgtcgagcca ctggcgcagc tgcgtcgccg
     6361 cggcccggag gacccactcg ccgagcggga cgatcagccc acgctcctcg gccaccggga
     6421 tgaagacgcc cggcgggatc agtccccgga cggggtcctt ccagcggacg agcgcttccg
     6481 cgccgacgat ccgccccgtc tccacctcca cctgcggctg gtagtggagg aggagctcgc
     6541 ccttcgcgag cgcctgccgc agcctcgtct cgatctccag ggtctccatc gcccgagccg
     6601 tcatctcccg gcggaagtac tcgagcgttc ccccttcgcc cgacttcgcg tgccggagcg
     6661 ccgcgtccgc gttccgcatc agcgtcggtg catcctcgcc gtcctccggg ctgaccgcga
     6721 ccccgcagct cgccgagatc gggatcgacc ggccggcgac ctcgaacggc gactcgaaaa
     6781 tcgcctggac cttccgggcc gcgatctcgc acccggccgt ccccttcacg ctcacgaagg
     6841 cgaccgcgaa ctcgtcgggt ccgatccggg ccaggacgtc gcgcgtccgg accgcgccgg
     6901 cgagccggct cgcgatggaa cggaggatct cgtcgccgac cgggccgccg agcgactcgt
     6961 tgatcgccga gaagcggtcg acgtcgagcg cgacgagggc gagcgcgtgc ccctcgagcc
     7021 gcgacgaggc gatcgcccgc gccagctgcg tctcgaggcc cgggcggttc acgaagtggg
     7081 tgagggggtc ccgctgggag aggaactcga tcctctcgat cgccgcgacc cgctccgtca
     7141 ggtcggtata gagggcgacg taatggctcg tcgctccccg ctcgtccttc acggccgtga
     7201 tcgacaggag gaccggatag acctcgccgt tgctcctccg gttgtggatc tctcctcgcc
     7261 accgccccgt ccgggcaatc gtcgcccaca tctgacggta gaacgcccga tcgtggtgtc
     7321 ccgacccgag gaaccggggg gtcctcccga gcacctcctc cgccgcgtat ccgctcatcg
     7381 tctcgaaggc ccggttcacg agcaggatgc gctggtcggc cccggccagc aggacgccgt
     7441 cggcgctccc ctcgaggacg tgcgaccaga ggcgctgccg ctcctcggcg agcttcgtct
     7501 ccgtcacgtc ccggacgacg ccgaagacgc cgacggggcg gccggagtcg tcgcggacga
     7561 gctccgaggt cgcgttcagc cagcggacct cgccggaggg gtcgaggacg cggtactcgt
     7621 ggcctccggc cgcctgaccg tcccagttcg ccgccgcgag cgcgcgcatc cgctcccggt
     7681 cgtccgggtg gacccgctcg aggaacgact cgttcgacgg ggtgaaggtc tccggcgtca
     7741 cgccgaagag ccggtagtgc ccgtcggacc agctgagctg ctgcgtctcg aggtcgccgt
     7801 agaagctccc ggtccgcccg atcgtctccg cgtcggaaag ccggcgggcc gtcaccttca
     7861 gcgcctcctc ggcgcgctgc cgctcgacct ccctgtcgcg cgccgcgagg gcgaagccga
     7921 ggtcttcggc gaggcccgcg agaagccgga cgatctcgtc gtcgaagtag cccttccgat
     7981 gcgccccgac ggcgagcgcg cctcgcggcc ccgcggctcc ggggatcggg aacgcggcga
     8041 gcgactcgag cccgaagcgc cgcgccgcct cgtgccagac ggtcgtcgag gcgtcggaga
     8101 ggtagtcgtt cgcgacgacg ggatgccctt cgcgaatggc gtcgacggtc ggcccgtggc
     8161 ccgagggcgc ctccgggtcg agcgtcagtg cggcctcccc gatgatcgcc gccgccggcc
     8221 cggcgacggc gagcgcccgg agctccccgt cggcggtcgc ctccgagacc catacgagac
     8281 ggaagtcggc cacctccacc gcgatctcgc agacccttcg ggcgagacgg accgactcgg
     8341 gcgtccggac gatcgccgcg ttcgcccgcg agaggagctc gtagagctgg ttcagccggt
     8401 ggatccgctc gtccgattcc ttccgttccg tgatgtcgcg aatgacgtac tggacgaagg
     8461 ggcgaccgtc gacctcgatc cgccgcccgc tgaagtcgac cggaaagagg gagccgtcct
     8521 tccgctggtg gatcgtctcg aggtggatgg aaccgtccgc gaggagcctc gcgaaagcct
     8581 cccgggcggc cggacggtcc gcggcgggcc ggaggatcgt cgtgtcggcc ccgatcagct
     8641 cctttcggga atagccgtac gtgtcgaagg cctttcggtt cgcctcgacg atccggaacg
     8701 actcgtcggt caggagcacg aggtcgttcg aggcttccga cagccacgcg aagtggcgcg
     8761 ccacggcgtc ccgttcggcc gcgagctccc tctcccggaa ggcgtgccgc acccgaaggc
     8821 cgcgccacca cgaaagcgtc gctccggccg ccgctgcgag gagagcgagg ttgaagagcg
     8881 cgatcccggc gagtcgtcgc cggagcggcc ggtaggcctc ctcggcgtcc cgccggacga
     8941 gaagcgtcca gtcggtgccg gagacgcggc ggagggcgcc gaagctcgcg acaccgcggt
     9001 cgtcgacctc ttcccgggcg ccgtcctccc cgcgtgcggc ccgcgccgcg agcgagtccc
     9061 ccacggcgga cacccggcgg gtccgcagcg tgtcgccggt ttctgcggcc acgacgaccg
     9121 cctccccgcc ctcccggcgg acgacgaccg cctcctcgct cgggctcgct ccgggagccc
     9181 gagcgaggag cgcgcgcagc tgctcgcgcg ccggcgcccc gacgacgacc cagcccggga
     9241 cgttgccgcc cggcacggcg gcgctcacgg ccgccactgc ccggattacg agcggcagcc
     9301 cctcctcggt ccccggctcg gcgaggagcg cctcgggcgt cgtccgtgtc gactccagcg
     9361 cctccttcag gcggggtccg aggcggatcg gcggccccac ctgctccacg acctcgcctg
     9421 cggcgtcgag gagcgcgacg gagcggcccg ggtggtgctc cgcgtagcgg gagagctcgg
     9481 ccccgaggtc gcgccgggag ccttcggaat cgggcccggc gcccgcggag aggaccgcct
     9541 cgacctccgc ctcgccggcc agcagccggg cgtcgatccg aagggcgtcg agccatcccg
     9601 aggcctgcgc gatcttcacc tccgccgtcg cggacagcgt cgcgagggtg gtcgcccgct
     9661 tctcggcgat ggcccgctcg tggagcaccc agcccgcccc ggccacggcc gccaagagaa
     9721 cgacgaagac ggcgacgggc gcccacgccc ccgcggtcag ccgctcgccc ctttcgagat
     9781 cgacctcggc gccgaaccgc tgcgccgatc ggaccgcgat cgcgaagacg aagaccgcgc
     9841 tcagggcgac gaagaggatc cccttcgcgc tctgcagggt cgtcatccgc tccgggtcga
     9901 cgatcgcccg gacgacgtcg tcggagaaga cgacccagag gactccgagc gcgccatacc
     9961 cggccgcggc ggcgagcgcc gtgcgtcgca ctccgggtgc cgccgatctg gagcgcccct
    10021 ctgccgtcat gccgtcgctc gcggtgccgc accgctcgtg gcgagtatag gcgtcgggag
    10081 gcgggcggac cgggaggaag ggacccggcg cggccgtcct ttcgccgtca gcttccggcc
    10141 tggagcttcg accagtcgat ctgcgatccc caggcgctgt gcggcacgag gaagacggcg
    10201 agcgtcacga gcgcggcgat gccgacggcg aggcgggcgt cgcgtcccga gcgcgcccgg
    10261 aatgcggcga cggcccaggc gaggacggcg aaggccgtct tgttgtcggt caggtcggtc
    10321 ccgaagggcc agccggtcca gtacgccccg aacgccgcct tctggacgag cggcccgagg
    10381 aagagcccgc cgagggcgag gaggacgagg gcgacgaccg aaatgccgag gaggcgcttc
    10441 gccccgcgca gcgcgaggag ccccgcggcg ttcgcgacga gcatcccccc gaacatcgcg
    10501 acgatgtgcg gaacgaggac gaccggcgag acggccccct tgaagcgggt gatcgcgggg
    10561 cgctccggga agagcgccgt ctccccgtcg cgggcgaggc gcacctggta ctccagcttc
    10621 ccggcgggag gctggtgcgg cagcgccgcc cggagcgtct ccccgtcgcg gaccatcgga
    10681 agcatcgtga acgcgtcgcg cgtcgggtag cgccgccagg cgacgcttcc cgtgaccgcc
    10741 cggtcggcga ccctcacgac gatcggctgg tccccttctc cgccgtgaga gcggaggagc
    10801 cggaatcgga cctcctcgcc gccgagcgtc accgccccgc ggaccggatg ggtcggcccg
    10861 gtcatccgct ggaagaccgc cgagccgagg gtgaggagga gggcgaggag ccagagaccg
    10921 attcgaccgc gcatgcgcgg cggaggatag cccgccagct ggcatcatcc ccgccatgcg
    10981 ctcccgcgtc gccccgggcg ccgtcgccgc ccggctcgcc ctcttcggtc tcctcctcgt
    11041 cccagcggcg acctcccggg ccgccggcga ggccgtcccg cccgtcgtct ccggaggctg
    11101 gctcgagccg aagctcgcgt cggcggagat cgtcctcctc gacgcccggc cgctccgcga
    11161 cttcctcgcc tcgcaccttc ccggcgccca gagcgtcgcc gtggagaacc tccgcgcgac
    11221 gcggggcggc gtaccggcga cgctccaccc cccggagctc ctcgggacga tcttcgccgg
    11281 cgcgggcgtc acgccgcgcg cccacgtcgt cgtctacggg gccgagagcg acgtcgacgc
    11341 cacgtacgtc gccaccgccg cccgcgtcgc cggggcgacg agggtcagcg tcctcgacgg
    11401 aggcttcgcc cgctggagcg ccgacctccg ccccgtccgg gtcgagcgga cgcgcttccc
    11461 ggtaccggtc gcgcggctgc gcggcgagcg gcggctcgtg atcgacctcg aggaggtcgc
    11521 gaagcgcgtg ggcgacggga ggaccgtcct cctcgacgtc cgccaggcgg cccagtgggc
    11581 gaaggagcgg atcccgggcg cgaagaatcg cccctggaag gcggacgtct ccgacggcct
    11641 cttccgaccc gccgatcggc tccgcgccga ctacgaggcc gcgggcgtct cctgggagaa
    11701 gccggtggtc gtctactgca acagcggcca ccaggcctcc gaggcgttct gggtcctgaa
    11761 gtacgtcctc ggccaccccg acgtccggct ctaccagggc tcctggctcg agtggtcgat
    11821 gaccccgggg acgccccgtg aaggcgaccg cccggcggag gagcggtgac cccgggcgag
    11881 atcgcggccc tcctcgcctc ctcgttcggt gccggcgtga tgaacgccct cgccggcggc
    11941 ggcacgatcc tcaccttccc gacgctcgtc ctcctcggca tccccgccat ccaggcaaat
    12001 gccacgtcca ccgtcgcgct cctccccgga gccgccgcga gcatggcggg ctaccggcgc
    12061 gaggtggcga cgcacgcggc gtggctgaag accctcttcc tcccgagcct cgtcggcggg
    12121 gcgatcggct ccgtcctcct cctgaagacg ccggagaagc tcttcgcgag cctcgcgccg
    12181 ttcctggtcc tcttcgcgac gctcctcttc ctcctccaaa tcgcgctcgc gaagcggaag
    12241 ggggacgccg aggtccacgc gcacgacggg ccgacgacgg cccgctgggg cgtcgcgacg
    12301 ctcgcgcagc tcgccgtcgc gatctacggc ggctacttcg gcgccggcat cggcatcctc
    12361 atgctcgtcg tcctcggctt cctcggcctc accgacatcc acgcgatgaa cggcctgaag
    12421 aacttcttcg ggatcacgat caacctcgtc gcggcggcct ggttcatctg gaacggggcc
    12481 gtcctctggc cgatcgccct cgtcatggtc gccggagcga cggtcggcgg ctacgcgggc
    12541 gcgcgcttcg cccggcggat cgggaaggag aaggcgcggg cggccgtcgt cgtcatcggc
    12601 ctcggcgtca ctgtggtgct cctcctccag cgggcctgaa gccgagcttc cgcgcccgct
    12661 ccgccaccag cgagaggtgc gcccgcaccc ccgtgtcagt cgcggcgagg tcccgggcgc
    12721 cgtcgagggc ctccgtcgcc aggacacgcg cttcgtctct ccgcccgagc gcggcgagga
    12781 gctccgcacg cagggagacg aagagcggcg cgacgtcgag ggcttcgcgt ccgacccgct
    12841 cgacgtcgac gaggagcgcg tcggcagcct cgcccccggc acgtcgcgcc tcgaggcccc
    12901 agtaccggaa gagcccgatg tcggcgacgc tgtcagcata gcgcgccggc ctgcgtccgc
    12961 tccgccaggc gacgaaggcc cgggccgcgg cgacgtcccg gacgcgatcg gggtaggccg
    13021 cctcgaaacg atcgacctcg gccgccgcgt cgtcccatcg ccccgcctcg ccgagcgcga
    13081 ggacgacccc cagcatcagg tcgcgcgggt agatcccgat ccgggcgtgg tcgccccggc
    13141 ccgtcaggcc ccggcgatac caggcgaccg cccgggcgag gtccccccgg aggtggaacg
    13201 cgcgccccgc ctcgagcgcg atccgcgccg gctcggccga cctctcgaac gaggcccccc
    13261 aggccgcgtc ggcttccccc ggctgtctcg cgtccaggag ggcgcgggcc cgcgcgcgcg
    13321 cgatccactg ccccagcggg gcgtctcccg ccgcgcgcgc cttctcgagc gcccgctcca
    13381 cggccacgag cgcttcccgg gcgagccccg ccccgcgcag ccgcatcccc tcgcggagga
    13441 gcccgtacgc cgcctcccgc gcgtcggagc gccgtttcgc ctcgcccggc tcgcgcggcc
    13501 cgcgcgggaa gccgtcgagc gcgagctcca cctgccgcgg gccccacgcg ccaacgcgca
    13561 ggaacccggc cgacgcctcg aggacctgcc cctcgtcgcg atcgaaggcg gcgtccggga
    13621 cgagggcgta ccaggcgagg agcgacttct cgtcccgcca ggccgggcga tcgggcgact
    13681 cggcgaacgg gacctccgac caggctcgcc ttccctcccc ttctcccggg atccggagcg
    13741 cggcgaccgc ggcggagtag cccatcctgt tgttgaggcc ggccaggatc agctccttcc
    13801 gcccgtcgcc gtcgacgtcg acggccgccc gcgcgtagtg gtggcccgag ccgtagaacg
    13861 cggcgacgac gcgccgccgg ccggggtcgc agacgtacgt cgctcccgcc cagagcggga
    13921 gatgggcgag gctcacggcg acctcgtcgt caccgtcgcc gtccacgtcg aaagggaaga
    13981 cggccccgac ggcgaacgtg ctcgagaagc ccgggaacgc ccccgccccg tcccggtcgc
    14041 cggccgggtc ggggagctcc atgtcggcga gggggcggcc ggtgaggccg tcgaggacga
    14101 cgacgcgctt cctcggatcg tccgtcccgc ccggcaccgc gatggccccc gtctcgcctc
    14161 cggaaagcct cgcgagcgcg atctggcggc tcgccgccgt gcccgcccgc ctccagagga
    14221 cgcgcccctt cccgtcgagc gcttgtaccc ctcccgcgcc gtcgggggcg aggcgcgtca
    14281 ggcgcggccg gttccacggg aagaccggga cgaacgcgag ggtgaggagg agggcgacga
    14341 gcgcggccga gcccccgagc gcgagggccc tcccgagacg tcgtggcggc cgccgagccc
    14401 ggcgggcctc caggtcggcg aggacctcgg tggccgtcgc gtagcgccgc tcgcggtcct
    14461 tcgcgaggag ccgcccgacg agggccgaaa gccacgcggg cgcctccggg cggagtttcc
    14521 tcacgtcgac cggctcctgc cggatgtgcg ccacgatcgt cccgatcgcc gactcgccgc
    14581 ggagcgggac cttgcccgcg agcatctcga agaggacgac gccgaccgcg tagaggtcgc
    14641 tccgcgcgtc gagcaggtcg ccgagcgcct gctccggcga gaggtactcg accgtcccga
    14701 cgagcgcctc cgtctcggtc acccgcgtct cgctcccctc ccagtggcgg gcgaggccga
    14761 agtcggcgag cttcacctct ccggcgtccg agaggaggat gttcccgggc ttcacgtcgc
    14821 ggtggacgat cccgagggcg tgaagatcgg cgagcgcccg gaggacctcc gcggcgatcg
    14881 cgacggcccg ctccggcggc agcggcccgc gcgcgaggag ctcgcggagc gactcccctt
    14941 ccacgagctc catcgtcagg aagaccgtgt cgcccgcctg accgatgtcg aagacgcgga
    15001 cgaggcgcgg gctggccgcg tcgcgggcga tcgccacctc gcgtcggaag cgcttcagcg
    15061 ccgcctcgga cgtccggtcg gtccgcagga ccttcaacgc gacgcgccgc ttcagctccc
    15121 ggtcgaacgc ctcgtagacg accgccgagc ctcccgtgcc gaggacgccg cgnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccggcgg ctcggcgccc gggcgccacc
    15301 ggagcgacgt cgggttgaag aggaccgtcc cgtcgggacg cggctccatc aggcggagga
    15361 agacgacccg ctcgcccccg tcgtcccgga cgttcagcgg caggctcgtc accccctggt
    15421 cgaggatccg gtcgtagttg aggagctgga aaaggaccgg cgcgccgggc agcagcagcc
    15481 gcgcgaggtg acccaggaac gacgccatct ccttctcgtg gaggtgcggc aggccgttcc
    15541 cgagcgaaat cgctcccccg aacggccccg tcaccgcggc gtcgagctcc gcgaggtccc
    15601 ccttcacgaa ctccggcccc ggccccgcct cccgcgcctg ggtgagctgc gcctccgacg
    15661 cgtcgacgcc cgtcacctcg aagccctcct ccgacaggaa ccgcgcgtgc tcgccggtgc
    15721 cgcagccgag gtcgaggagc ctccgcgacg gcgcgttcgc gaggacctcg cggaggaacg
    15781 gcgcctcccg ctcgatgcgc gcgggccagg cgatgagccg ccggtaggag aggcgggcgt
    15841 aggggtcgct cggatcggag ccgtcggaca tgaacggagt ttcgcaccgc gtccggagcg
    15901 ccggaggctc gacggcgcgc ggacgtgacg gaccgacctc ttccccgttg cgggcaaatg
    15961 gggccgtgtc tgcactcgtg tattgtctga cctcggcgac gcgagcgggg cagcggcgtt
    16021 gacgcgcacc ggctccgcgc cgtacgcggt tcctcgtgat gccggggagg caagggggcc
    16081 cgtgtggcat catcccggac ctgtcgcgga cgtcgccggt cggggtgacg cgggccgcga
    16141 ggaaagggcg ctcgatggcg cggaagggcg gcggaagggg cgggctcgcc agggcatcga
    16201 cggacgccgc ccgcagcctc aaccgcgaat cgagccgtcg gcggcgatgc atcgtcgcag
    16261 ccaccgcggc gatcttcggc gcggcggggc tcctcgcgat cgggtacagg ctcctgtccg
    16321 gaccgcggca ggctccaatc attctcatct cgatagacac cctgcgcgcg gaccacctgc
    16381 ccgcctacgg ctacacgaag atcccgacgc cggcgatcga cgagctcgcc cggaacggcg
    16441 tcgtgttcga gaactgctac acccacgtcc cgttgacgct gccggcgcac gcttcgctcc
    16501 tgacggggct ccttccaccc gatcacggag tccggaacaa catcggcttc cgggtggcgg
    16561 agggaacgcc gacactcgcg tccgagctga agcgggtcgg ctacgcgacg ggcggggcga
    16621 tctcggcggc cgtcctccga cgcgggacgg gcatcgccca gggattcgac tggtacgacg
    16681 acgacctgag ggccgagggc ccggaggtcc gcacgacgga ggtccaacgc gacggaagcg
    16741 agaccctcga gagggcgctt tcgtggctga gagagcgtgc gaatgcctcg agcggtgagc
    16801 cgccgttcct gttcttccac attttcgagc cgcacacgcc gtacgcccca cccgctcggt
    16861 accgacgcgc cggagtctcc ccgtacgacg gcgaggtcat gtacgccgac gagatcgtgg
    16921 gccggctctt cgacgagctc cgcaggctcg acctcttcga gcgcagcctg atcgtcctgc
    16981 tctcggatca cggcgaaggc ctcggcgacc acggagaggc agaccacgga gtcctcctct
    17041 atcgcgaggt gatgagggtg cccttcatcg tgaagctccc ggggcaggag ggcggaggaa
    17101 ggcgggtgaa cgatccggtg cagctcgtcg acctcaaccc gacgctgagg gagtgggtcg
    17161 ggcttccgcc gaggccggag cagcgcggca cctctctcct cgccacgctc cggggagggc
    17221 cggcgcccgg cgacaggcgc gtctacgggg agagcctgta cggacggctc catttcggct
    17281 ggaaggagct cttcagcctc accgactcgc ggttcaagct catcctcgcg ccgcggccgg
    17341 agctctacga cgtcgaaaag gacgcgctcg agcgccacga cctgctcgcg ggcggttcac
    17401 ccggcgtgga agcgtcgacc gagcgagacc ggcttcatcg cgcgctccgc gtcctcatcg
    17461 gaagcggggc gccgaaggcc cccaacccgg tcgacgagga agaggtcgcc cggctccggg
    17521 cgctcgggta cgtcgggtct ccgactctcg atccgaccga ggacgccacg gccccggacc
    17581 cgaaggaccg gatcggggac ctcgcgctct tcaggaaggc aatgcgcctg aagggcgtgg
    17641 ggatgtaccg gccggcggcc gaggacctcg agaaagtcgc ggcctcgagc ccgaggatgg
    17701 tcgatgcctg ggacgagctc ggtggcatct accgacgcct cggtcaggcg gagaaggccg
    17761 tggacgcttt caggagcggg ctcgcggtgg cgccctcgag gggtgaggcg cacctcgccc
    17821 tcgccgattt cctcgtcgag ctgggccggc tggacgaagc ccggcgggag gccgacgccg
    17881 ccgcgccgta ccgcccgggc gaagcggagg tccggcgcgc cgtcatcgag gcgacggccg
    17941 gagacccggc ggcggcgcgc gcgtccgcgc tgcgcgcgac gaatgcgctc cccgccgccg
    18001 ctccattcgt cgacgggctc ctcgcgcacg ctcgcgagga atacgccacg gcggtcccgc
    18061 ttctcgagaa ggctctcgac gcagtcaagg ggcgctcgcc ccgaacgctg ccgcgcgtcc
    18121 actatctcct cggcagcgcg ctcgtcgagc agagcgtcca cgtgtcggac gaagcgtccc
    18181 ggacacggct ccggcaggaa gcggagcggc acctccggac cgagctcgcg ctggagccga
    18241 cgagcgccgg cgcggtgacc gccctctgcc gcgttcacgc cctggcgggc gccccggaga
    18301 agctgcgtcg cgacctggaa gcgttcgcgc gagggaaccc gtctcccgcg gtcttccggc
    18361 tcgccgagac catcttcggt caggcgggcc tgaaggacga cgcggcccgc tggggcctgc
    18421 gcgctcgcgc gagcgccggg cgatcgcggc cggccctgcc ggcgatcgcg ccccgcccct
    18481 gacgacgagg ggcaacccga aggggcgcgc ggccggggtc gcccccggcc gcgccgatct
    18541 acctctccct gggtcgagtg cgcggggcgc cgtcgcggcg ccccgcacgg gctctacgcg
    18601 ccgccgccct tgtcggcccc cttgggccgc ttcgtgacct tggcgccgat gtagtcgcgg
    18661 ttcatccgcg cgatgacctc gagcttgatc tccttcgggc aggcggcctc gcactccccc
    18721 atgttcgtgc agccgccgaa cccctcggcg tccatctgct cgaccatctt caggacgcgc
    18781 tcctgccgct cgggctggcc ctgcgggagg aggccgaggt gggagacctt cgctccgacg
    18841 aagagcatcg ccgaggcgtt cggacaggcc gcggcgcagg cgccgcagcc gatgcacgcc
    18901 gccgcgtcca tcgcgagctc ggcgttctcc ttcggcaccg ggatggcgtt cgcgtcgacg
    18961 ccgacgcccg tgttcaccga gacgaagccg cccgccgcga tgatccggtc gtaggccgag
    19021 cggtcgacga tcaggtcctt gatgaccggg aacgccttcg ccatccacgg ctcgacggtg
    19081 atcgtctggc cctcgtggaa gctcctcatg tggagctggc aggtcgtcgt cgccgcgcgc
    19141 ggcccgtgcg ggacgccgtt gatgacgagc gagcaggcgc cgcagatccc ctcgcggcag
    19201 tcgtggtcga tcgcgatcgg ctcgtcgccc ttctccatga ggcgctcgtt cacgaggtcg
    19261 agcatctcga ggaaggagga gtcggtgctc acgcccggga cgtcgtagga gacgaggccg
    19321 cccttcgtgc ccggtccctt ctgccgccag atgcggagct tgatgttgat gtccttcgac
    19381 ccgctcatca cttgtagctc ctcgtggcga ggtggacgga ctcgaactgg agctgctccg
    19441 tgtgccgctc cggggtcgag ccctccccct tccactccca ggccgcgacg tgggcgaact
    19501 tctcgtcgtt acggagagcc tcgccgtccg gcgtctggta ctcctcgcgg aagtggccgc
    19561 cgcacgactc ctcgcgggcg agggcgtcgc ggcagatcag ctcgtcgagc tccaggaagt
    19621 cggcgagccg acccgccttc tcgagcgaga cgttcaggtc gtcgccgctc cccgggacga
    19681 agacgttctt ccagaactcc tcgcggatct ccgggatcct cgcgatcgcc tccttcaggc
    19741 cggcctcgtt gcggcccatg ccgcacttct cccacatgag gaggccgagc tgcttgtgga
    19801 tcgagtcgac cgtccgcttg cccttgatcg agaggagctt cttcacgcgg tcgttcacgt
    19861 cggcctcggc ggccttcacg tccgggtggg aggcgtcgat cttcgcgggc ttgccctgcg
    19921 cgaggtagtt gccgatggtg tacgggatga cgaagtagcc gtccgcgagc ccctgcatga
    19981 gggccgaggc gccgaggcgg ttcgcgccgt ggtcggagaa gttcgcctcg ccgaggacga
    20041 agagccccgg gaggttcgac atcaggttgt agtcgaccca gaggccgccc atcgtgtagt
    20101 ggacggccgg gtagatccgc atcgggacct cgtacgggtt ctcgtcggtg atcctctcgt
    20161 acatctcgaa gaggttcccg taccgctcgc ggatgacgct ctcgccgagc cggccgatcg
    20221 actcggcgaa gtcgaggtag acgccctgcc cgccggggcc cacgccgcgc ccctcgtcgc
    20281 agacctgctt ggccgcgcgg gaggagatgt cgcggggggc gaggttcccg tagctcgggt
    20341 acttgcgctc gaggtagtag tcgcgctcgg cctcggggat gtcgcccggc ttgcgcgtgt
    20401 cacccttctt caaggggacc cagatccggc cgtcgttgcg gagcgactcg gacatgagcg
    20461 tcagcttgct ctggttctcg ccgtggaccg ggatgcaggt ggggtggatc tgcgtgtagc
    20521 aggggttcgc gaagaaggcg ccgcgccggt gcgcgcgcca gatggcggtg ccgttgcagc
    20581 ccttcgcgtt cgtggagagg aagtagccgt tgccatagcc gcccgtcgcg agcatgacgg
    20641 cgtcggcgag gtggctcgag accttgccgg tgaccatgtc gcggacgacg atgccgcgtg
    20701 cgaccccgtc gacgacgatg aggtcgagca tctccgtccg cgggtacatc ttcaccttgc
    20761 cgaggccgat ctgccgctcg agcgcctggt aggcgccgat gaggagctgc tgccccgtct
    20821 ggccccgcgc gtagaacgtc cgcgagacct gcgcgccgcc gaacgagcgg ttcccgagcg
    20881 tgccgccgta ctcgcgcccg aacggcacgc cctgggcgac gcactggtcg atgatgtcgt
    20941 tgctcacctc ggcgaggcgg tagacgttcg actcgcgcgc gcggaagtcg cctcccttga
    21001 ccgtgtcgta gaagaggcgg tagacgctgt cgccgtcgtt ctggtagttc ttcgcggcgt
    21061 tgatgccgcc ctgcgcggcg atggagtgcg cgcggcgcgg gctgtcctgg tagcagaagc
    21121 aggagacgtt gtagccgagc tcggcgaggg acgcggcgcc cgaggcgccc gcgaggcccg
    21181 agccgaccat gatgaggctg tacttccgct tgttggccgg gttgatgagc ttgatggccg
    21241 ccttgtgccg cgtccagcgc gactccatgg ggccggtggg gcacttgccg tcgagttcca
    21301 tcccgcttcc tcctacttca ccaggcccgc gaggaccgcg atcgggaagg agacgttcac
    21361 gacgacgatc acggtgcaga gaacggcgag gaccagcttg atcggcttct cgtacttcgg
    21421 cgtccggagg cccagcgtct ggaagaggct gaagccgccg tggaagaggt ggaagccgag
    21481 ggcgagcatc gcgaggacgt agaagcccga gacccagacg ttcgagaagc cgacgacgac
    21541 gttcgtgtag acgtcggtgt ggctgaactg cg
//
LOCUS       DAOONL010000057        21452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2388_length_21452_cov_14.446464, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000057 DAOONL010000000
VERSION     DAOONL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21452
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_21452_cov_14.446464"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..690
                     /gene="rplM"
                     /locus_tag="PLP50_09935"
     CDS             283..690
                     /gene="rplM"
                     /locus_tag="PLP50_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPA51911.1"
                     /translation="MNELPRRWIVVDADGKVLGRLATVVAMHLTGKSKPTYTPFLDTG
                     DFVVVVNAEKVALTGVKETAKLYRYHTGYPGGLKTTSADKLRATRPEKIVEEAVRGML
                     PKTKLGKKMFKKLKVYAGPSHPHVAQVSKPVSA"
     gene            707..1099
                     /gene="rpsI"
                     /locus_tag="PLP50_09940"
     CDS             707..1099
                     /gene="rpsI"
                     /locus_tag="PLP50_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPA51912.1"
                     /translation="MSNLQYHAIGRRKEAVARVYLRPGSGAFVVNDRPFEKYFPNDVL
                     KMVIRQPLQLTESTEKFDIVARVAGGGSAGQAGAIRHGLARALCLFNLELRPSLKKAG
                     LLVRDSRMKERKKYGQRGARARFQFSKR"
     gene            1102..1914
                     /gene="rpsB"
                     /locus_tag="PLP50_09945"
     CDS             1102..1914
                     /gene="rpsB"
                     /locus_tag="PLP50_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPA51913.1"
                     /translation="MSQITMKELLEAGVHFGHQTKRWNPKMKKYIFGKRNGIYIIDLQ
                     KTLKCFREAAAFVTEAASQGKNILFVGTKRQAQDAIYEEANRCGMFFVNNRWLGGTLT
                     NFVTIRKSIMRLKEIEGMLAENSEAILTKKERIRLERERTKLAKNLSGITEMEELPDL
                     LFVIDPKKEAIAVQEANKLGIPIVGIVDTNCDPDPITHVIPGNDDAIRAIKLFTGKIA
                     DAVLEGLHAQEERFVGKAAEGDDEPMPANFVVTEGAAAVEAIASKDTAPTQA"
     gene            2056..2973
                     /gene="tsf"
                     /locus_tag="PLP50_09950"
     CDS             2056..2973
                     /gene="tsf"
                     /locus_tag="PLP50_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPA51914.1"
                     /translation="MEITTAMIKELREKTGAGILDCKSALAEANGSIEEAEKSLRKKG
                     LAAAAKKAGRVAAEGLVGYACDGSIAALVELNCETDFVAKTDDFKAARAALAAFVAAE
                     VSFGDAPEGNAEALKAMPCPPAVPGATGTIADWLQAATAKTGENTQVRRFARVVAGAG
                     NALHLYAHPGDKTVVVVETTGCDAELGRDLAMQVAALTPSWAVREQVPEKVLADEREI
                     ARAQAVASGKPENVVEKMVEGRIGKFFGEAVLVDQPFVKDDSKKVAQVVAERGGKDAK
                     VVRFVRYKVGEGVEKKQTDLAAEVAALTK"
     gene            2986..3729
                     /gene="pyrH"
                     /locus_tag="PLP50_09955"
     CDS             2986..3729
                     /gene="pyrH"
                     /locus_tag="PLP50_09955"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPA51915.1"
                     /translation="MGSETGRPVYRRVLLKLSGEALLGGQPFGIEPAMVARLADEVTG
                     VARMGVQVAVVIGGGNIIRGVSAATQGIDRVTGDNMGMLATVINALALQDAAERRGVP
                     TRVMTAFEVRSVAEPFIRRRAERHLDKGRVIILAGGTGNPFFTTDSAAALRASELRAD
                     AILKGTKVDGIYSADPKTDPAAVLLPHVTYQEVLEKRLGVMDAASIALCRESRIPIRV
                     FNLLVPGNIQRVVTGEPIGSTVDAGEPAN"
     gene            3755..4315
                     /gene="frr"
                     /locus_tag="PLP50_09960"
     CDS             3755..4315
                     /gene="frr"
                     /locus_tag="PLP50_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPA51916.1"
                     /translation="MPLAEIRKETERRMTTSIDALKAELKHLRTGRASVSLLEGVHVE
                     YYGTPTPLNQVANLSTPDATLILIQPWEAQLCPLIEKAIRNSDLGLNPSSDGRVVRVP
                     VPSPTEERRKEIVKKAHTLGEQTKVEIRHHRHEANDKIKKEGKASAVSADEEKKGLDD
                     IQKLTDRYVAEVDAVVKAKEADILAR"
     gene            4323..5003
                     /gene="ispD"
                     /locus_tag="PLP50_09965"
     CDS             4323..5003
                     /gene="ispD"
                     /locus_tag="PLP50_09965"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPA51917.1"
                     /translation="MAVVALIPAAGLGTRFHDAGPKALVRLAGRPFLLHALERLAASR
                     RVDRAVVAVSPGHEAAFAAALEGFRAMPVATTPGGETRRDSVVAAWRASGAADDDLLC
                     VHDAARPLVDPADVAAVVDAAAEHGAALAGSPVTDTVKRLRDGFVAGTVPRYDLFAAA
                     TPQVMRAGLLREAVEAGLADATDDVELLERRGVPVRAVVTSRWNVKITFPEDLRRAEA
                     WLAAEGRR"
     gene            5000..5473
                     /gene="ispF"
                     /locus_tag="PLP50_09970"
     CDS             5000..5473
                     /gene="ispF"
                     /locus_tag="PLP50_09970"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPA51918.1"
                     /translation="MSLRIGQGLDAHPLVAGRRCVLGGVEIPSDAGPEGHSDGDVVLH
                     ALADALLGAAAAGDLGSVFGTTDPRFRDAPSSVFVTEALARAGSPKVVNVDVTLIAER
                     PRVGTHRDAMRAVIAALLGIPVERVSVKASSGNGMTGFGRGEGVFASAVVLVEVP"
     gene            5470..6195
                     /gene="rlmB"
                     /locus_tag="PLP50_09975"
     CDS             5470..6195
                     /gene="rlmB"
                     /locus_tag="PLP50_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPA51919.1"
                     /translation="MIVAGFHPVAAALAERPEAVEALLLQDGRRDARARELERTARER
                     GIPVRFVPREELDRTAGKAHNGVAARVATRDYDPLEEALAGEPGTRLVLFLDEVTDPG
                     NLGSILRSAAAAGASVVLPERHSVGLSESVSKTSAGALERVKVARVGNAARFLEDVKK
                     DRFWVFGASPEGEPMWDVDLTGDVVLCLGAEGPGLRRLTRETCDRLVAIPMAPGAGSI
                     NVAVSAAVLLFEALRQRRPPPGR"
     gene            complement(6219..9053)
                     /locus_tag="PLP50_09980"
     CDS             complement(6219..9053)
                     /locus_tag="PLP50_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51920.1"
                     /translation="MRRFLLVPVGVVVLLSFRLHAVELVVPVVLDVRSGAAHYRTELT
                     LTNGAALPVLLGFAYRGSLGDAAGSVSAGIGAGTQWTIPDVIAWLEGHGVVFPGLRDG
                     VPHAGTLRITCPDEQAATLAAVARTTTPTSAPHPEGAAGLAYPASRTDLAFTGRAVVH
                     GLRAGMTERSNLAVWNPGGSPVTVRITVTSGTGDGQEVVVTEGWSLGPGDWKQVDGIL
                     AKAGLPQGWAIVERVAGGAFSAYGVVNDGGTNDGSFVEAAPDAAAPGAIVVPVLVETP
                     GFRTELILANRGTTAAPLEVDYRESLSRSEGEGGRATVTLAPREQRIISDAFAWLRGL
                     GVAVGPAGPAPLAGRLRVTGPPGATGLWAAARVASPSPGGGAYGVFLPGVRPDPASGA
                     GAWIAGLRSDGEVRSNVAFVHAGPPGSGAVELEFRVFDGLAGGAPAGAPARVALAEGE
                     WHQLADPLRPLGVASGWVRVTRIAGTAPWLAYGVLNDGAGPGLRTGDGAYVPAVASAS
                     TSAARIAPDDFSYLGAFRLPAGGERPLTFEYGGNAMTFRPGGDPGGAGDGFPGSLFVS
                     GHDRMPYGELPDGSRVAEVSIPAPLVSRDVETLPVAAFLQPLTEVTGGLFAGIDEIPR
                     LGMQYLDRPETGPRIHLAWGEHLPPPPARATHAWFEPDLSAPRPRGAWFLDGLSPWEG
                     NGYMLEIPDAWANAHVSGRVLGTGRYRDGGMAGMGPALFAYRPWLDAAGTPPASGATL
                     PVTPLLRYASTLETERVERCLVGTQHGDTWEGAAWLTTASGRSAVLFAGTKGVGARFW
                     YGFLNPAGPDLPCVAGDFVNEYTTCRLADGTPCAASEMVECAGHTSSRGWWSSRFASR
                     LLLYDPDDLARVASGEAEPWEPQPYAHLDVDPLLYLNPSGVDLADIGPGVQQRFRLGD
                     VTFDRASGLLYLLELFADGARPVVHVFRVR"
     gene            9235..9310
                     /locus_tag="PLP50_09985"
     tRNA            9235..9310
                     /locus_tag="PLP50_09985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9268..9270,aa:Thr,seq:tgt)
     gene            9379..9466
                     /locus_tag="PLP50_09990"
     tRNA            9379..9466
                     /locus_tag="PLP50_09990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9413..9415,aa:Tyr,seq:gta)
     gene            9528..9603
                     /locus_tag="PLP50_09995"
     tRNA            9528..9603
                     /locus_tag="PLP50_09995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9561..9563,aa:Thr,seq:ggt)
     gene            9669..9743
                     /locus_tag="PLP50_10000"
     tRNA            9669..9743
                     /locus_tag="PLP50_10000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9701..9703,aa:Gly,seq:tcc)
     gene            9816..9971
                     /gene="rpmG"
                     /locus_tag="PLP50_10005"
     CDS             9816..9971
                     /gene="rpmG"
                     /locus_tag="PLP50_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPA51921.1"
                     /translation="MPRDLISLACGECKRRNYTTTKNKKTHQEKLEVSKYCRFCRKHT
                     AHKEGKV"
     gene            9986..10061
                     /locus_tag="PLP50_10010"
     tRNA            9986..10061
                     /locus_tag="PLP50_10010"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10019..10021,aa:Trp,seq:cca)
     gene            10142..10354
                     /gene="secE"
                     /locus_tag="PLP50_10015"
     CDS             10142..10354
                     /gene="secE"
                     /locus_tag="PLP50_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPA51922.1"
                     /translation="MNLTERWKTFREFLVDVRKETGKVSWPAKDEVVGTTTVVILYTV
                     VVGVFLFAVDATITPAINWFFAAFGQ"
     gene            10431..10958
                     /gene="nusG"
                     /locus_tag="PLP50_10020"
     CDS             10431..10958
                     /gene="nusG"
                     /locus_tag="PLP50_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPA51923.1"
                     /translation="MDWYIVHTYSGFEDRVVQELTNRIKAQGMESQFGEIRVPKETVV
                     EMKAGKRRNVERKFFPGYVLVQMEMADETWHLVRNTPKVTGFVGGSSKSPVPLMPEEV
                     EAILHHTAESKEKPKPKYTYERGEKVKIVDGPFKEFTGDVEEINIERSTLRVLVTIFG
                     RPTPVELEFVQVQKL"
     gene            11031..11456
                     /gene="rplK"
                     /locus_tag="PLP50_10025"
     CDS             11031..11456
                     /gene="rplK"
                     /locus_tag="PLP50_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPA51924.1"
                     /translation="MAKKVVGQVKLQIEAGKATPAPPVGTALGPQGVNIMDFCKAFNA
                     KTQKDAGLIIPVVVTVYSDRSYSFILKTPPASVLLKKAAKVEKGSGVPNKNRIGKVTM
                     QQVEEIAKLKLVDLNAADLEAAKKTVAGTARSMGIEVIG"
     gene            11617..12327
                     /gene="rplA"
                     /locus_tag="PLP50_10030"
     CDS             11617..12327
                     /gene="rplA"
                     /locus_tag="PLP50_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPA51925.1"
                     /translation="MTEGKKYTAAAAKIDRTKLYDVQQAVALVRSAAYAKFNETLEVA
                     LRLGVDPKHADQMVRGTVVLPHGTGQTIRVAVVASGEKVREAEDAGADVVGGDDLVAK
                     IQGGYLDFEALVATPDMMKSLGRLGKVLGPRGLMPNPKAGTVTFDVGKAVKELKAGKI
                     EFRVDKTAIIHCPVGKLSFADAALVENTEALIAAVQKAKPSAAKGKYVKSIFLGSTMG
                     PSVPVDPAVADKVAGRAA"
     gene            12324..12848
                     /gene="rplJ"
                     /locus_tag="PLP50_10035"
     CDS             12324..12848
                     /gene="rplJ"
                     /locus_tag="PLP50_10035"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPA51926.1"
                     /translation="MNRTEKTDAIAQMTEALGSAPHAFLIDFQGISVPDVTELRRQIR
                     ATGSEYVVVKNTLALRAIKDAPIGALSEHFTGMTAVAWSKTDIVQLAKVLHQFGKTNP
                     KIKVKAALVEGRPVPAAALESLASLPTRPELVARLLGLMQSPVRRLVTVLSSPQRKLA
                     MTISAVAEKKSAAA"
     gene            12919..13299
                     /gene="rplL"
                     /locus_tag="PLP50_10040"
     CDS             12919..13299
                     /gene="rplL"
                     /locus_tag="PLP50_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPA51927.1"
                     /translation="MALSVEQFVSEVEGMSVLELNNLVKALEEKFGVSAAMMAAPAAA
                     AAPAAAAAPAEEQTEFTVSLKEAGGNKINVIKAVREVNSSLGLKEAKDLVENLGVLKE
                     SVSKDEAAAIKKKFEEIGAKVEIK"
     gene            13455..17729
                     /gene="rpoB"
                     /locus_tag="PLP50_10045"
     CDS             13455..17729
                     /gene="rpoB"
                     /locus_tag="PLP50_10045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPA51928.1"
                     /translation="MADRMLAPRQTARHDFGKIKTFLPLPNLIDVQRRSYEDFLQMDL
                     LPHERDDVGLEAVFRGVFPFSDFRETCALEYVSFRIGDWASRSGRLEGIQHLRFTCEH
                     CGETVRVKDPRANTVGCQACGHPNPNKVTIDEVCGTPVELRLPFTVAECQERGLTYAV
                     PLKVTFRLKVFDKDNAPTGKSAPKEWPIRDIKEEEVYFGDIPLMTDNGTFIINGTERV
                     IVSQLHRSPGVFFTREGAHTLIGKIIPYRGSWVEFEIDSKGLFGVRIDRKRKFPGTVF
                     LRALGLESDEQILKKFYSAVGLTFDKGKAHLVLPPDVLAKEEIRQKHVRGVRKDGRIL
                     ADVKLTAEQREQLASGQSVTVTADPAKLGRILFAVDVVDLSSGEVLYEAGTEVPDDLA
                     TVMTEKQATPVEVLFPDWEPIGETLIATLRKDSVKTKREALLEIYKRMRPGDPPTDES
                     AKNLFVGMFFDPRKYDFSRVGRYKFNIRMGLSTSLEQKTLSSDDFYVVIDALLKLRKD
                     IGKTDDIDNLGNRRVRCVGELLENQFRIGLVRMERAIKEKMSVHQDIDSAMPHDLINS
                     KPVIAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLSRERAGFEVRDVHAT
                     HYGRICPIETPEGPNIGLISSLSCYARISEYGFIESPYKKVRDGRVLEHFEIFTVGDS
                     GWKKGAIVEASELEAMNAKLKKAGKRLAVGRPTAFYLPAWEEEDYAIAQANEPLEADG
                     RFVNDRVQVTLQGEFSAKPRETVHFMDVSPKQLVSVAAALIPFLEHDDANRALMGSNM
                     QRQSVPLLRTEPPIVGTGLEGVVARDSGAVVLCKRDGIVDTVDARRIIVRVEGIGPDG
                     KPREFGADIYPLLKFRRSNQNTCINQKPIVKEGQRVRAGEVLADGPCTSGGELALGRN
                     ILVAFMPWRGYNFEDAILISEKMVKEDYYTSIHIEEFEIEARETKLGPEEITKDIPNV
                     SEHALRDLDESGIIRIGANVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRD
                     ASLKCPPGIEGVVVDVKIFTRKDDEKQRDVRAKQILDEEIGFLNKDAEDEIRIVRTEA
                     KEKIERLLEGRTVLEDVVDPQGNVLVAANHKLTVEKIGMMGPRLLKSLRLRDEEVKDT
                     VRLVLQRADSQVDVIRKVAADRVALLSKGDELAPGVNKTVKVYVAMKRKLQVGDKMAG
                     RHGNKGVISRILPEEDMPYLPDGTPVQIVLNPLGVPSRMNVGQILETHLGWAAQALGL
                     SIASPVFDGASEEEIRALLEKSNLPRSGKTELYDGMTGDRFEQKVTVGYIYMLKLSHL
                     VDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTVKSD
                     DVDGRSRVYEAIVKGEVPEEPGLPESFNVLVRELQSLCLDVELLKV"
     gene            17741..>21452
                     /gene="rpoC"
                     /locus_tag="PLP50_10050"
     CDS             17741..>21452
                     /gene="rpoC"
                     /locus_tag="PLP50_10050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPA51929.1"
                     /translation="MNLDTFGASPLSPAVPAFRGPGPRDFNAIKIALASPEKIRSWSF
                     GEVTKPETINYRTFKPERDGLFCAKIFGPITDWECLCGKYKRMKHRGVVCDKCGVEVT
                     KSKVRRERMGHIELAAPVSHVWFFKGLPSRIGHLLDLTLRDLERILYFEAYVVVDPGP
                     LAEEENVNEKALITEERYRDLRAKYGADAFVAKMGAEAIQELLQRVNIDDLADELRLI
                     MKTETSALKRLKAAKRLKVVDAFRRSGHRPEWMILNVIPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVINRNNRLKKLLELRAPEVIVRNEKRMLQEAVDALFDNGRRGRVLKGTN
                     NRPLKSLSDTLKGKSGRFRQNLLGKRVDYSGRSVIVVGPELKLNQCGLPKRMALELFK
                     PFIYRRLEQKGLTSTIKMSKELVEAAPNEVWDALEEVIKEHPVLLNRAPTLHRLGIQA
                     FEPVLIEGKAIKIHPLVCAAFNADFDGDQMAVHVPLSPKAQVEAQVLMLSAHNILSPA
                     HGKPLAVPSQDLVLGLYYLTRRKEGCKGEGRVFPGFDDVLLAHGAGEVDTHAGITVRY
                     TGRLVDLESQVLGDQDIVHAEPIDCEGTRIETTVGRVLMCAHLPKDIPFINGTLKKKG
                     LQDLVNYCFVNLGLERTVEILDIIKQLGFTAATRAGISIGVDDMRIPSKKTGFVDKAR
                     KDVLEVDNQRAQGAITDGERHNKIIDIWHRATEAVSEAMMKEMKAEDAAPGNEFNPIF
                     MMADSGARGSKEQVRQLAGMRGPMSKPSGEVMEQPIISNFREGLSVLEYFISTHGARK
                     GLADTALKTADSGYLTRRLVDVAQDVIINEEDCATLDGITVTAIVEGGEILEPLRDRI
                     VGRFVQEDVYDPATDEVVIAANDLITEEIATAIQEAGIEKVKIRSVLTCETRRGVCRK
                     CYGRNLATGRMVDIGEAVGVIAAQSIGEPGTQLTMRTFHYGGTARVSETARHVAKNPG
                     VARFLNALTVRKNDGTEVVVNRNSKLVIQDAKGREKERYSLAYGSVLKVTSGEEVKPG
                     QLLVTWDPFASPILSEIEGKVVFKDILEGENLREETDKVTGLSQKIVVETTGTTEKRS
                     PQLVVLGADGTERRYALPIPAHLMVADGDTVRPGDALAKKPVEKKRTRDIVGGLPRVV
                     ELFEARRPKEPAVITEVSGAVRHAGTVKGQQKLVVESEDGQKRDILVPRGAHVMVQEG
                     ERVEAGDPLTEGDPSPHDILAVKGEKELQRYMT"
BASE COUNT         3699 a         7482 c         7414 g         2857 t
ORIGIN      
        1 cagctcggac atttctatcg agctggcacc gtgacatttc catggagctt cgacactttt
       61 ctccgggtca cgcttgacag gccggaggcc gcgtcgtatg tttcccggcc ctgcgcgagg
      121 gcgaccgcgc gcaaggtgcg tcctcccccg ggagccggtc tttctcagtg tcccggccgg
      181 gtcctgaata ccaagagacg agaagcgtgg ctcgttgcgc gcttcgccga accccgaccg
      241 gagagctgag cttccgtgag ttcccgaacg cctctcccca agatgaacga gctcccgcgg
      301 cgctggatcg tcgtcgatgc cgacggcaag gtcctgggcc gcctcgcaac ggtcgtcgcg
      361 atgcacctga cgggcaagtc gaagccgacc tacacgccgt tcctggacac cggcgacttc
      421 gtcgtcgtgg tcaacgccga gaaggtcgcg ctcacgggcg tcaaggagac cgccaagctt
      481 taccggtacc acaccgggta cccgggcgga ctcaagacga cgagcgccga caagctccgc
      541 gcgacccgtc ccgagaagat cgtcgaggag gccgtccggg ggatgcttcc gaagacgaag
      601 ctcggaaaga agatgttcaa gaagctgaag gtctacgccg gcccgagcca cccgcacgtg
      661 gcccaggtct cgaagccggt ttcggcgtga gtcgcgagga gagatcgtga gcaacctgca
      721 gtaccacgcg atcggccggc ggaaggaagc ggtcgcccgc gtctaccttc gtccggggag
      781 cggggcgttc gtcgtcaacg atcgcccgtt cgagaagtac ttcccgaacg acgtcctcaa
      841 gatggtcatc cgccagcccc tccagctgac ggagtcgacc gagaagttcg acatcgtcgc
      901 ccgcgtcgcg ggcggcggga gcgcgggcca ggcgggcgcg atccgccacg gcctcgcgcg
      961 cgccctctgc ctcttcaacc tcgagcttcg gccgagcctg aagaaggcgg gcctcctcgt
     1021 gcgcgactcg cggatgaagg agcggaagaa gtacggccag cgcggcgccc gcgcccggtt
     1081 ccagttctcg aagaggtagc catgagccag atcacgatga aggagctcct cgaggcgggc
     1141 gtccacttcg ggcaccagac caagcgctgg aacccgaaga tgaagaagta catcttcggg
     1201 aagcgcaacg gcatctacat catcgacctg cagaagacgc tgaagtgctt ccgcgaggcg
     1261 gcggccttcg tcaccgaggc ggcctcccag gggaagaaca tcctcttcgt cgggacgaag
     1321 cgccaggccc aggacgccat ctacgaggag gcgaaccgct gcggcatgtt cttcgtgaac
     1381 aaccgctggc tcggcgggac cctcacgaac ttcgtgacga tccgcaagtc gatcatgcgc
     1441 ctcaaggaga tcgaggggat gctcgccgag aactcggagg cgatcctcac gaagaaggag
     1501 cgcatccggc tcgagcgcga gcggacgaag ctcgcgaaga acctctccgg catcaccgag
     1561 atggaggagc tcccggacct cctgttcgtg atcgacccga agaaggaggc gatcgcggtg
     1621 caggaggcga acaagctcgg catcccgatc gtcggcatcg tcgacacgaa ctgcgacccc
     1681 gacccgatca cgcacgtcat ccccggcaac gacgacgcga tccgggcgat caagctcttc
     1741 accggcaaga tcgccgacgc cgtcctcgag ggcctgcacg cccaggagga gcggttcgtc
     1801 gggaaggccg cggaggggga cgacgagccg atgcccgcga acttcgtcgt gacggagggc
     1861 gcggccgccg tcgaggcgat cgcctcgaag gacaccgcgc cgacgcaggc ctgatcctcg
     1921 accgatcctg atcgagccgg gccggcggga gcagcggatg gcgcgctccc gccggccctc
     1981 ttcacgtccg gcagccccgg gcgcacgaaa cgaaagaaac cgcgcgggag agcccgcgcg
     2041 cgacgggaga gcccgatgga aatcaccacc gccatgatca aggagctccg ggagaagacc
     2101 ggggccggaa tcctcgactg caagtcggcc ctcgccgagg cgaacggctc gatcgaggag
     2161 gccgagaagt cgctccgcaa gaaggggctc gccgccgccg ccaagaaggc cggccgcgtc
     2221 gccgccgaag ggctcgtcgg ctacgcctgc gacggctcga tcgccgcgct cgtcgagctg
     2281 aactgcgaga ccgacttcgt cgcgaagacc gacgacttca aggccgcccg ggcggccctc
     2341 gcggcgttcg tcgccgccga ggtctccttc ggcgacgccc ccgaggggaa cgccgaggcg
     2401 ctcaaggcga tgccgtgccc gccggccgtc cccggggcga ccgggacgat cgccgactgg
     2461 ctccaggccg ccacggcgaa gacgggcgag aacacccagg tccgccggtt cgcccgcgtc
     2521 gtcgccggtg cggggaacgc gctgcacctc tatgcccacc ccggcgacaa gaccgtcgtc
     2581 gtcgtcgaga cgaccggttg cgacgcggag ctcggccgcg acctcgccat gcaggtcgcc
     2641 gcgctgacgc cctcctgggc cgtccgcgag caggtccccg agaaggtcct cgccgacgag
     2701 cgcgagatcg cccgggccca ggccgtcgcc tcgggcaagc cggagaacgt cgtcgagaag
     2761 atggtcgagg gacggatcgg gaagttcttc ggcgaggcgg tcctcgtcga ccagccgttc
     2821 gtcaaggacg actcgaagaa ggtagcccag gtcgttgccg agcgcggggg caaggacgcg
     2881 aaggtcgtcc gcttcgtccg ctacaaggtg ggcgagggcg tcgagaagaa gcagaccgac
     2941 ctcgccgcgg aggtcgcggc gctgacgaag tagaacggga cgccgatggg cagcgagacg
     3001 ggccggccgg tctaccgccg ggtcctcctc aagctctccg gggaggccct cctcggaggg
     3061 cagcccttcg gcatcgagcc ggcgatggtc gcgcgcctcg ccgacgaggt gaccggcgtc
     3121 gcccggatgg gcgtgcaggt cgccgtcgtc atcggcggcg ggaacatcat ccgcggcgtc
     3181 tcggccgcga cgcaggggat cgaccgggtg accggcgaca acatggggat gctcgccacg
     3241 gtcatcaacg cgctcgcgct ccaggacgcc gcggagcggc ggggcgtgcc gactcgggtg
     3301 atgacggcct tcgaggtccg ctccgtcgcc gagccgttca tccggcgacg ggcggagcgg
     3361 cacctcgaca aggggcgcgt catcatcctc gcgggcggaa cgggcaaccc cttcttcacg
     3421 accgactcgg ccgcggcgct ccgggcgagc gagctgcggg ccgacgcgat cctgaagggg
     3481 acgaaggtcg acggcatcta cagcgccgac ccgaagaccg atcctgcggc cgtcctcctc
     3541 ccgcacgtca cctaccagga ggtcctcgag aagcggctcg gcgtcatgga cgccgcctcg
     3601 atcgccctct gccgggagag ccgaatcccg atccgggtct tcaacctcct cgtccccggg
     3661 aacatccagc gcgtcgtgac gggcgagccg atcggctcga cggtcgacgc gggcgaaccc
     3721 gccaactgaa tcgagaccag agaggagaca gccgatgccc ctcgccgaga tccgcaagga
     3781 aaccgagcgc cggatgacga cgtcgatcga cgccctgaag gccgagctga agcacctccg
     3841 gacgggacgg gcttccgtct cgctgctcga aggggtccac gtcgagtact acgggacgcc
     3901 gacgccgctg aaccaggtgg cgaacctctc cacgccggac gcgacgctga tcctcatcca
     3961 gccctgggag gcgcagctct gcccgctcat cgagaaggcg atccgcaaca gcgacctcgg
     4021 cctgaacccg agctccgacg ggcgggtcgt ccgcgttccg gtcccctctc cgaccgagga
     4081 gcggcgcaag gagatcgtca agaaggccca caccctcggc gagcagacga aggtcgagat
     4141 ccgccaccac cgccacgaag ccaacgacaa gatcaagaag gaagggaagg cgagcgcggt
     4201 ctcggccgac gaggagaaga agggcctcga cgacatccag aagctgaccg accggtacgt
     4261 ggccgaggtc gacgcggtcg tcaaggcgaa ggaagcggac atcctcgcgc ggtgaccggg
     4321 acgtggccgt cgtcgcgctg atcccggcgg cgggcctcgg cacgcgcttc cacgacgcgg
     4381 ggccgaaggc gctcgtgcgg ctcgcgggga ggccgttcct cctccacgcc ctcgagcgcc
     4441 tcgccgcctc gagacgcgtc gaccgcgcgg tcgtcgccgt ctcgccgggc cacgaggcgg
     4501 ccttcgcggc ggctctcgag ggcttccggg cgatgccggt cgcgacgacg cccggcgggg
     4561 agacgcggcg cgactccgtc gtcgccgcgt ggcgcgcctc gggggcggcc gacgacgacc
     4621 tcctctgcgt ccacgacgcg gcgcgcccgc tcgtcgaccc ggccgacgtc gcggccgtcg
     4681 tcgacgcggc ggccgagcac ggggccgccc tcgccgggag ccccgtgacc gacacggtga
     4741 agcggctccg cgacggcttc gtcgccggga cggttccgcg gtacgacctc ttcgccgcgg
     4801 ccacgccgca ggtcatgagg gccggcctcc tccgcgaggc ggtcgaggcc gggctcgccg
     4861 acgcgaccga cgacgtcgag ctcctcgagc ggcgaggtgt tcccgtccgc gcggtcgtca
     4921 cgtcccgctg gaacgtgaag atcacgtttc ccgaggacct ccgacgagcc gaagcgtggc
     4981 tcgccgcgga ggggcgtcgg tgagcctgcg gatcgggcag ggcctcgacg cgcacccgct
     5041 cgtcgcgggg cggcgctgcg tcctgggcgg agtcgagatc ccgtcggacg ccggccccga
     5101 gggtcactcg gacggcgacg tcgtcctcca cgccctcgcg gacgcgctcc tcggcgcggc
     5161 ggccgcaggg gacctcggga gcgtcttcgg gacgaccgac ccgcgattcc gcgacgcccc
     5221 gtcgtccgtc ttcgtgaccg aggcgctggc ccgcgcgggg agtccgaagg tcgtgaacgt
     5281 ggacgtcacg ctgatcgccg agcggcctcg ggtcgggact caccgggacg cgatgcgagc
     5341 cgtcatcgcc gcgctcctcg ggatccccgt cgagcgcgtc tccgtgaagg cctcgagcgg
     5401 caacgggatg acgggcttcg ggcgcggcga gggcgtcttc gcctccgccg tcgtcctcgt
     5461 cgaggtcccg tgatcgtcgc cgggttccac cccgtggccg cggcgctcgc cgagcggccg
     5521 gaggcggtcg aggcgctcct gctccaggac ggacggcgcg acgcgcgggc gcgcgagctg
     5581 gagcggacgg cgcgggagag aggaatcccg gtgaggttcg tcccgcgcga ggagctcgac
     5641 cggacggcgg ggaaggccca caacggcgtc gccgcgcgcg tcgcgacgcg ggactacgac
     5701 cctctcgagg aggcgctcgc cggcgagccc gggacgcgcc tcgtcctctt cctcgacgag
     5761 gtgaccgacc cggggaacct gggctcgatc ctccgctccg ccgcggcggc cggcgcctcc
     5821 gtcgtcctgc cggagaggca ctccgtcggc ctttccgagt cggtctcgaa gacgagcgcc
     5881 ggggcgctcg agcgggtgaa ggtggcgcgc gtggggaacg cggcccgctt cctcgaggac
     5941 gtcaagaagg accgcttctg ggtcttcggc gcctcgccgg agggcgagcc gatgtgggac
     6001 gtcgacctca cgggcgacgt cgtcctctgc ctcggcgccg aggggccggg tctccggcgg
     6061 ctgacgcgcg agacgtgcga ccgtctcgtg gcgatcccga tggcgccggg cgccggctcg
     6121 atcaacgtcg cggtctccgc cgcagtcctc ctcttcgagg cgctccggca acgccggccg
     6181 ccgcccggcc gctgagagac gctgcagggg cggcgtcctc accggacgcg gaagacgtgg
     6241 acgacggggc gcgccccgtc cgcgaagagc tcgagcaggt agaggaggcc gctggcgcgg
     6301 tcgaacgtca cgtcgccgag gcggaagcgc tgctggacgc ccggcccgat gtcggcgagg
     6361 tcgacgccgg acgggttcag gtaaaggagc ggatcgacgt cgaggtgggc gtagggctgc
     6421 ggctcccacg gctccgcctc cccggaggcg acccgcgcga ggtcgtccgg gtcgtagagg
     6481 aggagccgcg aggcgaagcg gctgctccac catccgcggc tcgaggtgtg ccccgcgcac
     6541 tcgaccatct ccgatgcggc gcacggcgtc ccgtccgcga ggcggcaggt cgtgtactcg
     6601 ttcacgaagt cgcccgccac gcaggggagg tccgggcccg cggggttgag gaagccgtac
     6661 cagaagcggg cacccacccc cttcgtcccc gcgaagagga cggctgacct cccggaggcc
     6721 gtcgtgagcc aggcggcccc ctcccaggtg tcgccgtgct gggtcccgac gaggcaccgc
     6781 tcgacccgct cggtctcgag cgtcgaggcg tagcgaagca ggggcgtgac cgggagggtc
     6841 gcgccggatg cgggaggcgt cccggcggcg tcgagccagg gcctgtaggc gaagagcgcc
     6901 ggccccattc cggccatgcc gccgtcgcgg tagcggcccg tgccgaggac acggcccgag
     6961 acgtgggcgt tcgcccacgc gtcggggatc tcgagcatgt agccgttccc ctcccacggc
     7021 gagagcccgt ccaggaacca ggctcccctt ggccgcgggg cggagaggtc gggctcgaac
     7081 caggcgtgcg tggcgcgggc cggcggcggc gggaggtgct ctccccaggc gaggtgaatt
     7141 cgcggccccg tctcgggccg gtcgaggtac tgcatcccga ggcgcgggat ctcgtcgatc
     7201 ccggcgaaga ggccgccggt gacctcggtc agcggctgga ggaacgccgc cacggggagc
     7261 gtctcgacgt cgcgcgagac gaggggcgcg gggatcgaga cctccgcgac acggctcccg
     7321 tcgggcagct cgccgtaggg catccggtcg tggcccgaga cgaagagcga gccggggaag
     7381 ccgtctccgg ctccacccgg gtcgcccccc ggccggaagg tcatcgcgtt ccccccgtat
     7441 tcgaacgtca agggacgctc tcctccggcc gggaggcgga aggcgccgag gtaggagaag
     7501 tcgtcggggg cgatccgggc ggccgacgtg gaagcgctcg cgacggccgg gacgtacgcg
     7561 ccgtcgcccg tacggaggcc gggtccggcg ccgtcgttga ggaccccgta ggcgagccac
     7621 ggcgccgttc ccgcgatccg ggtgacccgg acccatcccg acgccacccc gagcgggcgg
     7681 agcgggtcgg cgagctggtg ccactcgccc tccgcgaggg cgacacgcgc gggggctccg
     7741 gcaggcgccc ctccggcgag gccgtcgaag acgcggaact cgagctcgac ggcgcccgag
     7801 ccgggcgggc cggcgtggac gaaggcgacg ttcgaccgga cctccccgtc cgaccgaagg
     7861 ccggcaatcc aggctccggc cccggaagcc gggtcgggcc gaacgccggg aaggaagacg
     7921 ccgtacgcgc cgccgccggg agagggggaa gcgactcgcg cggccgccca gagcccggtc
     7981 gcgcccggcg gccccgtcac ccggagccgg ccggcgagcg gcgccgggcc ggccggcccg
     8041 acggcgacgc cgaggccgcg cagccaggcg aaggcgtcgg agatgatccg ctgctcgcgc
     8101 ggcgcgagcg tcaccgtcgc gcgtccgccc tctccttcgg aacgcgacag cgactcccgg
     8161 taatcgacct cgaggggcgc ggccgtcgtg ccgcggttcg cgaggatcag ctcggtccgg
     8221 aagcccggcg tctcgaccag gacggggacg acgatcgccc cgggggccgc cgcgtcgggc
     8281 gccgcttcca cgaacgagcc gtcgttcgtc cccccgtcgt tcacgacgcc gtaggcggag
     8341 aaggccccgc ccgcgacccg ctcgacgatc gcccagccct gcggaaggcc ggccttcgcc
     8401 aggatgccgt cgacctgctt ccagtctccc ggcccgaggc tccagccctc ggtcacgacc
     8461 acctcttggc cgtctccggt cccgctcgtc acggtgatcc ggacggtcac gggggagccg
     8521 ccggggttcc agacggcgag gttcgacctt tcggtcattc cggctctgag cccgtggacg
     8581 acggcccggc cggtgaaggc gaggtccgtc ctggaggccg gataggcgag cccggcggcg
     8641 ccttccgggt ggggtgcgga ggtgggcgtc gtcgtccgcg cgacggccgc gagggtcgcg
     8701 gcctgctcgt cgggacaggt gatgcggagc gtcccggcgt ggggcacgcc gtcccgaagg
     8761 cccgggaaga cgaccccgtg cccttccagc cacgcgatca cgtcggggat cgtccactgc
     8821 gtgccggccc cgatgcccgc cgagacgctc ccggcggcat ctccgagcga gccccggtac
     8881 gcgaagccga ggaggacggg gagggcggcc ccgttcgtca acgtcagctc cgtgcgatag
     8941 tgcgcggccc cgctccggac gtcgaggacc acaggaacga cgagctcgac ggcgtggagt
     9001 cgaaacgaca acaggacgac gaccccgacc gggacgagga gaaagcgacg catcggaacc
     9061 tccgacaggg cccgatcgaa tatggcctcg ggcgaagctg accgccagga agcccgttcc
     9121 gggtggtcgc ggcacgggac tcgcttgacg agaaggccga gcattgatat aagccacggt
     9181 tcgcgccttc cggcttcggc cgggtgtccg gccggaggcc ccggggcgcg acgggctggc
     9241 gtagctcaac tggcagagca gccgatttgt aatcggcagg ttgcgggttc aagtcccatc
     9301 gccagctcca cgggcgctcc cggaaggggt tccgggccgc tccgtgggaa ggagagagga
     9361 agagacggac gtaaaggcgg gtaggtggcc gagtggttaa tggcagcaga ctgtaaatct
     9421 gccgcgctta ggcgctacgg gggttcaaat ccctccctgc ccaccagcac ttccgacatc
     9481 ggctccggct ccgaggaggg gttcggagcg gccagtccag gggacgggcc cacgtagctc
     9541 aggcggtaga gcacgtcctt ggtaaggacg gggtcagcag ttcaattctg ctcgtgggct
     9601 ccagactcct ctctcgggcg atcggcccca ccggagagag ggcggaacga cggggacgaa
     9661 cgctacgggc gggagtaact caggggtaga gtcacagcct tccaagctgt tggtcgcggg
     9721 ttcgaatccc gtctcccgct ccaccccctt cccggggcgg ggacggacgg agagccggga
     9781 cgagccgcgg gggcggcgag gaagcggaga aagcgatgcc gagagacctg atcagcctgg
     9841 cgtgcggcga gtgcaagcgc cggaactaca cgacgacgaa gaacaagaag acccaccagg
     9901 agaagctgga ggtctcgaag tactgccgtt tctgccgcaa gcacacggcg cacaaggaag
     9961 gcaaggtcta gggacggacg ggcacagggg tgtagctcaa ttggcagagc aacggtctcc
    10021 aaaaccgtag gttcggagtt cgagtctccg cacccctgcc acatcccccg cctcgccagg
    10081 gcgggccgaa cgccccggag cgggcggcac ggcgcccgag ggcgccagga agacggatcg
    10141 aatgaacctc accgagcggt ggaagacctt cagggagttt ctcgtcgacg tccggaaaga
    10201 gaccggcaag gtcagctggc ctgcgaagga cgaggtcgtc ggaacgacga ccgtcgtgat
    10261 cctctatacc gtcgtcgtcg gcgtgttcct cttcgcggtc gacgcgacga tcacgccggc
    10321 catcaactgg ttcttcgccg ccttcgggca gtgatcccgg gaggcgcccc gcgatgatcg
    10381 agaacgacgg ctccccgacc ctttccgaag cgtcctcccc ggcgccggcg atggactggt
    10441 acatcgtcca cacgtactcg ggcttcgagg accgggtcgt ccaggagctg acgaaccgga
    10501 tcaaggccca ggggatggag tcgcagttcg gcgagatccg ggtgccgaag gagaccgtcg
    10561 tcgagatgaa ggcggggaag cgccgcaacg tcgagcgcaa gttcttcccc ggctacgtcc
    10621 tcgtccagat ggagatggcc gacgagacgt ggcacctcgt gaggaacacg ccgaaggtca
    10681 ccggcttcgt cgggggctcc tcgaagagcc ccgttccgct catgccggag gaggtcgaag
    10741 cgatcctcca ccacacggcc gagtcgaagg agaagccgaa gccgaagtac acgtacgagc
    10801 gcggcgagaa ggtgaagatc gtcgacggcc ccttcaagga gttcacgggg gacgtcgagg
    10861 agatcaacat cgagcgttcg acgctccgcg tcctcgtcac gatcttcggc cggccgacgc
    10921 ccgtcgagct cgagttcgtc caggtccaga agctgtaatc cgcgggagcc ggctcgccgg
    10981 ccgcccgaag aacgcggggc tcgccccgtc accactcgaa ggaacccaca atggccaaga
    11041 aagtcgtcgg gcaggtcaag ctccagatcg aagccggaaa agcgactccc gcgccgcccg
    11101 tcggcaccgc cctcggcccg cagggcgtga acatcatgga cttctgcaag gccttcaacg
    11161 cgaagacgca gaaggacgcg gggctgatca tcccggtcgt cgtgacggtc tattccgacc
    11221 ggtcctactc cttcatcctg aagacccctc cggcctccgt cctcctcaag aaggccgcga
    11281 aggtggagaa gggctcgggc gtcccgaaca agaaccggat cgggaaggtc acgatgcagc
    11341 aggtcgagga gatcgccaag ctgaagctcg tggacctcaa cgccgccgac ctcgaggccg
    11401 ccaagaaaac ggtcgcgggg acggctcgct ccatggggat cgaggtcatc ggctgagaag
    11461 aacgaaatcg tcgcgtgacg atttctcatt gagacggcga acgggccggc gccgcccctc
    11521 tcacggcgcg cggctccgcg atgcccgggc tgcctcccgg ggcttctcat cgcggggacg
    11581 caggacggtc gccgcactcg gaagagcgga agagaaatga cggaaggcaa gaagtacacc
    11641 gccgcggcag ccaagatcga ccggacgaag ctctacgacg tccagcaggc cgtcgcgctc
    11701 gtccgttcgg cggcgtacgc gaagttcaac gagacgctcg aggtggccct gcgcctcggc
    11761 gtcgacccga agcacgcgga ccagatggtc cgcggcacgg tcgtcctccc gcacgggacg
    11821 ggccagacga tccgcgtggc cgtcgtcgcc tccggcgaga aggtccgcga ggcggaggac
    11881 gccggcgccg acgtcgtcgg aggggacgac ctcgtcgcga aaatccaggg cgggtacctc
    11941 gacttcgagg ccctcgtcgc cacgccggac atgatgaaat cgctcggacg gctcggtaag
    12001 gtcctcggcc cccgcggcct catgccgaac ccgaaggccg gcacggtcac cttcgacgtc
    12061 ggcaaggcgg tcaaggagct gaaggccggc aagatcgagt tccgcgtcga caagaccgcg
    12121 atcatccact gcccggtcgg caagctctcg ttcgcggacg cggccctggt ggagaacacc
    12181 gaggcgctga tcgcggcggt ccagaaggcg aagccgtccg ccgccaaggg gaagtacgtg
    12241 aagtcgatct tcctcggctc gacgatgggg ccgagcgtgc cggtcgaccc cgcggtcgcc
    12301 gacaaggtcg ccgggagggc cgcgtgaacc gcaccgagaa gaccgacgcc atcgcgcaga
    12361 tgacggaagc cctggggagc gctccccatg ccttcctcat cgacttccag gggatctccg
    12421 tccccgacgt caccgagctg cgccgtcaga tccgcgcgac cggctcggag tacgtcgtcg
    12481 tcaagaacac gctcgccctg cgcgccatca aggacgcccc gatcggcgcg ctctccgagc
    12541 acttcaccgg catgacggcg gtcgcctggt cgaagaccga catcgtccag ctcgcgaagg
    12601 tcctccacca gttcgggaag acgaacccga agatcaaggt gaaggccgcc ctcgtcgagg
    12661 gacgcccggt cccggccgcg gccctcgagt cgctcgccag cctcccgacg cgccccgagc
    12721 tcgtggcgag gctcctcggc ctcatgcagt ccccggtgcg ccgcctcgtc accgtcctct
    12781 cgtcgccgca gcgcaagctc gcgatgacga tctcggccgt ggccgagaag aagagcgccg
    12841 cggcctgagg ccggacgcac acgaaacgaa tctggatacg cgcccgcgcg aagagacaag
    12901 gaaagcaagg agaacgaaat ggccctttcc gtcgagcagt tcgtcagcga agtcgagggg
    12961 atgtccgtcc tcgagctcaa caacctggtc aaggccctcg aggagaagtt cggcgtctcc
    13021 gcggccatga tggccgcccc ggccgccgcg gccgccccgg ccgccgccgc cgcccccgcc
    13081 gaggagcaga ccgagttcac cgtctccctc aaggaggccg gcggcaacaa gatcaacgtc
    13141 atcaaggcgg tccgcgaggt caactcctcc ctcggcctga aggaggccaa ggacctcgtc
    13201 gagaacctcg gtgttctcaa ggagtccgtc tcgaaggacg aggcggcggc catcaagaag
    13261 aagttcgagg agatcggcgc gaaggtcgag atcaagtagt ccggcgtgtc ccgcaacggg
    13321 aggaccgtcc ggtgacggag gtcgattccc cggggggccg gctcgtccgg ccccgcgggg
    13381 atcgcgccgt ctcgccggac ggtatttccc tctttccgcg tcaccctctt cccggatgaa
    13441 gggagcccgc tcgaatggct gaccgcatgc tcgccccgag gcagaccgcc cggcacgatt
    13501 tcggcaagat caagacgttc ctgcccctgc cgaacctgat cgacgtccag aggcggtcct
    13561 acgaggactt cctccagatg gacctcctcc cgcacgagcg ggacgacgtc ggtctggagg
    13621 ccgtcttccg cggggtcttc ccgttctccg acttccggga gacgtgcgcg ctcgagtacg
    13681 tctcgttccg gatcggcgac tgggcctccc ggagcgggcg gctcgagggg atccagcacc
    13741 tccggttcac ctgcgagcac tgcggcgaga cggtccgggt gaaggacccg cgggcgaaca
    13801 cggtcgggtg ccaggcctgc ggccatccga acccgaacaa ggtgacgatc gacgaggtct
    13861 gcggcacgcc ggtcgagctc cgcctgccgt tcacggtcgc ggagtgccag gagcggggcc
    13921 tcacgtacgc ggtccccctg aaggtcactt tccggctgaa ggtcttcgac aaggacaacg
    13981 ccccgacggg gaagagcgcc ccgaaggagt ggccgatcag ggacatcaag gaggaggagg
    14041 tctacttcgg agacatcccc ctcatgacgg acaacgggac cttcatcatc aacggcacgg
    14101 agcgcgtcat cgtctcgcag ctccaccgct ctccgggcgt cttcttcacg cgcgaggggg
    14161 cccacaccct catcgggaag ataatcccgt accgcggctc ctgggtggag ttcgagatcg
    14221 actccaaggg tctcttcggc gtccggatcg accggaagcg gaagttcccc gggacggtct
    14281 tcctccgcgc gctcggcctc gagagcgacg agcagatcct caagaagttc tactccgccg
    14341 tcgggctcac gttcgacaag gggaaggcgc acctcgtcct cccgccggac gtcctcgcga
    14401 aggaggagat ccgccagaag cacgtccgcg gcgtccggaa ggacgggcgg atcctcgccg
    14461 acgtcaagct caccgccgag cagcgggagc agctggcctc cggccagagc gtcacggtca
    14521 cggccgaccc ggccaagctc ggccggatcc tcttcgccgt cgacgtcgtc gacctttcct
    14581 cgggcgaggt cctctacgag gcgggtaccg aggtcccgga cgacctcgcg accgtcatga
    14641 cggagaagca ggcgacgccg gtcgaggtcc tcttccccga ctgggagccg atcggcgaga
    14701 cgctcatcgc gaccctccgg aaggactcgg tcaagacgaa gcgcgaggcg ctcctcgaga
    14761 tctacaagcg gatgcgcccc ggcgacccgc cgacggacga gagcgcgaag aacctcttcg
    14821 tcgggatgtt cttcgacccg cgcaagtacg acttctcgcg cgtcggccgc tacaagttca
    14881 acatccggat gggcctctcg acctcgctcg agcagaagac gctctcgtcc gacgacttct
    14941 acgtcgtcat cgacgcgctc ctgaagctcc ggaaggacat cggcaagacc gacgacatcg
    15001 acaacctcgg caaccgccgg gtccgctgcg tcggcgagct cctcgagaac cagttccgca
    15061 tcggcctcgt ccggatggag cgcgccatca aggagaagat gtccgtccac caggacatcg
    15121 actccgcgat gccccacgac ctgatcaact ccaagccggt catcgcggcg atcaaggagt
    15181 tcttcgggtc gtcgcagctc tcgcagttca tggaccagac gaacccgctc tcggaggtga
    15241 cgcacaagcg ccgcctgtcg gccctcgggc cggggggcct gtcgcgcgag cgcgccggct
    15301 tcgaggtccg cgacgtccac gccacgcact acggccgcat ctgcccgatc gagacgccgg
    15361 aaggcccgaa catcggcctg atctcctcgc tctcctgcta cgcgcggatc tccgagtacg
    15421 gtttcatcga gtcgccctac aagaaggtcc gcgacgggcg ggttctcgag cacttcgaga
    15481 tcttcaccgt cggcgactcg ggctggaaga agggggccat cgtcgaggcc tccgagctcg
    15541 aggcgatgaa cgcgaagctg aagaaggccg gcaagcggct cgccgtgggc cggcccaccg
    15601 ccttctacct cccggcgtgg gaggaggagg actacgccat cgcgcaggcg aacgagccgc
    15661 tcgaggccga cggccgcttc gtgaacgacc gcgtccaggt gaccctccag ggcgagttca
    15721 gcgcgaagcc gcgggagacg gtccacttca tggacgtctc gccgaagcag ctcgtctccg
    15781 tcgccgccgc gctcatcccg ttcctcgagc atgacgacgc gaaccgagct ctcatgggct
    15841 cgaacatgca gcgtcagtcg gtgccgctcc tgcgcaccga gccgcccatc gtcggcaccg
    15901 gtctcgaggg cgtcgtcgcc cgcgactcgg gcgccgtcgt cctctgcaag agggacggca
    15961 tcgtcgacac ggtcgacgcg cgccggatca tcgtgagggt cgaggggatc gggccggacg
    16021 gcaagccgcg cgagttcggg gcggacatct acccgctcct gaagttccgc cggtcgaacc
    16081 agaacacgtg catcaaccag aagccgatcg tcaaggaagg ccagcgcgtg agggcgggtg
    16141 aggttctcgc cgacggcccc tgcacgtcgg ggggcgagct cgccctcggg cggaacatcc
    16201 tcgtcgcctt catgccgtgg cgcggctaca acttcgagga cgcgatcctg atctccgaga
    16261 agatggtgaa ggaggactac tacacctcca tccacatcga ggagttcgag atcgaggccc
    16321 gcgagacgaa gctcgggccc gaggagatca cgaaggacat cccgaacgtc tccgagcacg
    16381 cgctccgcga cctcgacgag tcggggatca tccggattgg cgcgaacgtg aagccgggcg
    16441 acatcctcgt cgggaaggtc acgccgaagg gcgagaccca gctcaccccc gaggagaagc
    16501 tcctccgggc gatcttcggc gagaaggccg gcgacgtccg cgacgcctcg ctcaagtgcc
    16561 cgccgggcat cgagggcgtc gtcgtcgacg tgaagatctt cacgcgcaag gacgacgaga
    16621 agcagcggga cgtgagggcc aagcagatcc tcgacgagga gatcggcttc ctcaacaagg
    16681 acgccgagga cgagatccgc atcgtccgga ccgaggccaa ggagaagatc gagcggctcc
    16741 tcgagggccg cacggtcctc gaggacgtcg tcgacccgca ggggaacgtc ctcgtcgccg
    16801 cgaaccacaa gctcacggtc gagaagatcg gcatgatggg gccgcggctc ctcaagagcc
    16861 tccgactccg cgacgaggag gtcaaggaca ccgttcggtt ggtcctccag cgcgcggact
    16921 cccaggtcga cgtcatccgc aaggtggcgg ccgaccgggt cgctctcctc tcgaagggcg
    16981 acgagctcgc ccccggcgtc aacaagacgg tcaaggtcta cgtcgccatg aagcgcaagc
    17041 tccaggtcgg cgacaagatg gccggccgcc acgggaacaa gggcgtcatc agccggatcc
    17101 tccccgagga ggacatgccg tacctcccgg acggcacgcc ggtccagatc gtcctgaacc
    17161 cgctcggcgt cccgtcgcga atgaacgtcg ggcagatcct cgagacgcac ctcgggtggg
    17221 cggcgcaggc cctcggcctg agcatcgcct cgcccgtctt cgacggggcc tccgaggagg
    17281 agatccgggc gctcctcgag aagtcgaacc tgccgcggag cggcaagacg gagctctacg
    17341 acggcatgac gggtgaccgc ttcgagcaga aggtcacggt cggctacatc tacatgctca
    17401 agctgtccca cctcgtcgac gacaagatcc acgcccgctc catcggcccc tactcgctca
    17461 tcacgcagca gcccctcggc ggcaaggcgc agtttggcgg ccagcggttc ggcgagatgg
    17521 aggtctgggc gctcgaggcg tacggcgcgg cgtacacgct ccaggagctc ctcacggtca
    17581 agtcggacga cgtcgacggg cgctcccgcg tctacgaggc gatcgtcaag ggcgaggtcc
    17641 ccgaggagcc cggcctcccc gagtcgttca acgtcctcgt ccgcgagctg cagtcgctct
    17701 gcctcgacgt cgagctcctg aaggtctgag gagggaactg atgaacctgg acaccttcgg
    17761 cgcctccccc ctctcgcccg cggtcccggc gttccggggc ccggggcccc gcgacttcaa
    17821 cgcaatcaag atcgccctgg cgagccccga gaagatccgc tcctggtcct tcggcgaggt
    17881 gacgaagccg gagacgatca actaccggac cttcaagccg gagcgcgacg ggctcttctg
    17941 cgcgaagatc ttcggaccga tcaccgactg ggagtgcctc tgcggcaagt acaagcggat
    18001 gaagcaccgg ggcgtcgtct gcgacaagtg cggcgtcgag gtgaccaagt ccaaggtccg
    18061 tcgcgagcgg atggggcaca tcgagctcgc ggccccggtc tcccacgtct ggttcttcaa
    18121 gggccttccg agccggatcg gccacctcct cgacctgacg cttcgcgacc tcgagcggat
    18181 cctctacttc gaggcctacg tcgtcgtcga cccgggcccg ctcgcggaag aggagaacgt
    18241 caacgagaag gcgctcatca ccgaggagcg ctaccgggac ctccgggcga agtacggcgc
    18301 cgacgcgttc gtcgcgaaga tgggcgccga ggcgatccag gagctcctcc agcgggtcaa
    18361 catcgacgac ctcgccgacg agctccggct catcatgaag acggagacct ccgcgttgaa
    18421 gcgcctcaag gccgccaagc gcctgaaggt cgtcgacgcc ttccgccgct ccgggcaccg
    18481 gcccgagtgg atgatcctga acgtcatccc ggtcatcccg cccgagctcc gccccctcgt
    18541 gccgctcgac ggcggacgct tcgcgacgtc ggacctgaac gacctctacc ggcgcgtcat
    18601 caaccggaac aaccgcctca agaagctcct cgagctccgc gcgccggaag tcatcgtccg
    18661 caacgagaag cggatgctcc aggaggcggt cgacgccctc ttcgacaacg gccggcgcgg
    18721 ccgcgtcctg aaggggacga acaaccggcc gctgaagtcg ctctccgaca ccctcaaggg
    18781 gaagtcgggc cgcttccgcc agaacctcct cggcaagcgc gtcgactact cgggccggtc
    18841 ggtcatcgtc gtcggccccg agctgaagct gaaccagtgc ggcctcccga agcggatggc
    18901 cctcgagctc ttcaagccct tcatctaccg gcgtctcgag cagaaggggc tcacctcgac
    18961 gatcaagatg tcgaaggagc tcgtcgaggc ggcgccgaac gaggtgtggg acgccctcga
    19021 ggaggtcatc aaggagcacc cggtcctcct gaaccgggcg cccacgctcc accgcctcgg
    19081 catccaggcg ttcgagccgg tcctcatcga gggcaaggcg atcaagatcc acccgctcgt
    19141 ctgcgcggcc ttcaacgccg acttcgacgg cgaccagatg gccgtccacg tcccgctctc
    19201 gccgaaggcg caggtcgagg cgcaggtcct catgctctcg gcgcacaaca tcctctcgcc
    19261 cgcgcacggc aagccgctgg cggtgccgtc gcaggacctc gttctcggcc tctactacct
    19321 gacccgccgt aaggaagggt gcaagggcga gggccgggtc ttcccgggct tcgacgacgt
    19381 cctcctcgcg cacggcgcgg gcgaggtcga cacgcacgcc gggatcacgg tccgctacac
    19441 gggccgcctc gtcgacctcg agagccaggt gctcggcgac caggacatcg tccacgccga
    19501 gccgatcgac tgcgagggga cccggatcga aacgacggtc gggcgcgtcc tgatgtgcgc
    19561 gcacctcccg aaggacatcc cgttcatcaa cgggaccctg aagaagaagg gcctgcagga
    19621 tctcgtcaac tactgcttcg tgaacctcgg cctggagagg accgtcgaga tcctcgacat
    19681 catcaagcag ctcggcttca cggccgccac ccgggccggg atctccatcg gcgtcgacga
    19741 catgcggatc ccgtcgaaga agacgggctt cgtcgacaag gcgcgcaagg acgtcctcga
    19801 ggtcgacaac cagagggccc agggcgcgat caccgacggc gagcgccaca acaagatcat
    19861 cgacatctgg caccgcgcca ccgaggccgt ctccgaggcc atgatgaagg agatgaaggc
    19921 cgaggacgcg gctcccggga acgagttcaa cccgatcttc atgatggccg actccggcgc
    19981 ccgaggctcg aaggagcagg tgcgccaact cgccggcatg cgcggtccca tgtccaagcc
    20041 ttcgggcgag gtcatggagc agccgatcat ctcgaacttc cgggaagggc tctccgtcct
    20101 cgagtacttc atctcgacgc acggcgcccg caagggcctc gcggacacgg ctctcaagac
    20161 ggccgactcc ggctacctca cgcgccgcct cgtggacgtc gcccaggacg tcatcatcaa
    20221 cgaggaggac tgcgccacgc tcgacgggat caccgtcacg gcgatcgtcg aggggggcga
    20281 gatcctcgag cctctccgcg accgcatcgt gggccgcttc gtccaggagg acgtctacga
    20341 cccggcgacg gacgaggtcg tcatcgccgc caacgacctg atcaccgagg agatcgcgac
    20401 cgccatccag gaggcgggca tcgagaaggt gaagatccgc tcggtcctca cctgcgagac
    20461 ccgtcgcggc gtctgccgga agtgctacgg ccgcaacctc gccacgggcc ggatggtcga
    20521 catcggcgag gcggtcggcg tcatcgccgc ccagtcgatc ggcgagccgg gtacccagct
    20581 cacgatgcgg acgttccact acggcgggac ggcgcgcgtc tcggagaccg cccggcacgt
    20641 cgcgaagaac cccggcgtcg cgcgcttcct caacgcgctc acggtccgca agaacgacgg
    20701 caccgaggtc gtcgtcaacc gcaactccaa gctcgtcatc caggacgcca aggggcgcga
    20761 gaaggagcgc tactccctcg cctacggctc cgtcctcaag gtgacctcgg gcgaggaggt
    20821 caagcccggc cagctcctcg tcacctggga ccccttcgcc tcgccgatcc tctcggagat
    20881 cgaaggcaag gtcgtcttca aggacatcct cgagggggag aacctccgcg aggagaccga
    20941 caaggtcacg ggcctgtcgc agaagatcgt cgtcgagacg accgggacca ccgagaagcg
    21001 gtcgccccag ctcgtcgtcc tcggcgccga cgggacggag cgccgctacg cgctcccgat
    21061 cccggcccac ctcatggtcg ccgacggcga cactgtccgg ccgggcgacg cgctcgccaa
    21121 gaagcccgtc gagaagaagc ggacccgcga catcgtcggc ggtctcccgc gcgtcgtcga
    21181 gctcttcgag gcccggcgcc cgaaggagcc cgcggtcatc acggaggttt ccggcgccgt
    21241 ccgtcacgcc ggcacggtca aggggcagca gaagctcgtc gtcgagtccg aggacgggca
    21301 gaagcgggac atcctcgtcc cccggggcgc gcacgtcatg gtccaggaag gcgagcgcgt
    21361 cgaggcgggc gaccccctca ccgagggcga tccctccccg cacgacatcc tcgcggtcaa
    21421 gggcgagaag gagctccagc ggtacatgac cg
//
LOCUS       DAOONL010000058        21354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2411_length_21354_cov_21.541669, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000058 DAOONL010000000
VERSION     DAOONL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21354
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2411_length_21354_cov_21.541669"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..680)
                     /locus_tag="PLP50_10055"
     CDS             complement(129..680)
                     /locus_tag="PLP50_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51930.1"
                     /translation="MNEGPGPSGERLRAAFDAVHDLLESRYGIPVRIADVLDPNTGDF
                     DGVEIAVDHDQEVDVALFVLAHLFGHTVQWNVSEELRELGIAASSLDASPGPERLEAI
                     RRYEREASEYALALFHEAGVEDLDAWLSDWAEADWRYLSAAYAGKALPGEFRAHLRPG
                     AALLVPRSIPPFQPTRWVSRYSF"
     gene            complement(677..1603)
                     /locus_tag="PLP50_10060"
     CDS             complement(677..1603)
                     /locus_tag="PLP50_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51931.1"
                     /translation="MAEQQVPRNRSAAQRSAARSGVSPAVRAAAKETAVDLLKGRAAD
                     TVFGAQPSYVKAAFLNPYNLSLLGGAAAASLLTLNPLPILGALGLEALWLLWAPDSKR
                     LRHLLWDPRFAKLREAIEAEERRKRIAVLPEHDRERVEALVARRREIERLAAQNPTFT
                     GELLRGELVKTDRLVDAFVEMALTCARYRSYLDSVDARALERDVERYAAEVRRGDPED
                     QRTEISQKNLAIVVKRQERMREIDHYLAVARGQLDLIENTFQLIADQIVTMQSPKELS
                     GQLDELLEGVEAIKQSARDTEAMLEASGLSNP"
     gene            complement(1615..2421)
                     /locus_tag="PLP50_10065"
     CDS             complement(1615..2421)
                     /locus_tag="PLP50_10065"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA51932.1"
                     /translation="MSAAAPAAVPRLSVKDLSVTYIGRGGEKTEAVRDVSFDIADKPG
                     AGEIVVFLGPSGCGKSTILKAVAGLLPPTKGEVLLDGQPIGGPSQDRGMVFQAYTSFG
                     WLTVQENVEYGLKLRGVAAKERGDRAREVLKEVGLLDFAKRFPKDLSGGMKQRVAIAR
                     TLVNKPRVVLMDEPFGALDPMTRWGMQGLLLDVSRIEDNTILFVTHDVSEAVYLADTC
                     YVLSSRPARILHRVDVPNFAERDVHLKSTDEFRAIEKKLLDMLYAPQPAG"
     gene            complement(2418..3215)
                     /locus_tag="PLP50_10070"
     CDS             complement(2418..3215)
                     /locus_tag="PLP50_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51933.1"
                     /translation="MSNRGLFALRAPLPKRTALVLGFVMPALVLAVWCVLSYGGLAPA
                     DFLPSPTEVVRGTLQLFLQHDLWRAILVSTRRIALAFLIASAVALPLGVLMGAFTPVN
                     RLFEPIVAPLRYMPISAFIPLLILWFGIYEKQKIAFLFLGVFVYLLPVVVTAIRTVPE
                     EYVQTAMTLGATRGQVIRTVLLPAAAPEIFDSFRVMNAISWTYVILAEAVNPEHGLGY
                     MVELARTHQKASWSFAGLLVIGGIGLLTDFLISSVSRLLFRWREVNA"
     gene            complement(3339..4967)
                     /locus_tag="PLP50_10075"
     CDS             complement(3339..4967)
                     /locus_tag="PLP50_10075"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HPA51934.1"
                     /translation="MKLTPFAKLFITVVVLGVLGYAFWHYKGDTVRQWAGAEKKGAVE
                     SAGVDSADFDALKGAPADAERGKGSEGVTGAALTGSGKLGRPLVVAINTWAGHAPGVV
                     FNAGMDPNPTSYYKRKYGLDVKFVLLEDPAAKLAAFRKGDVDVMWNTVDNWAREASIL
                     AEQGAKAKSILLQDWSRGGDGIVSLASIKSIEDLRGKKVACTQFTPSHFLLLYLLSQS
                     GLTPEERAGVEKAIVFTQDAPAAAAMFKARQVDAAVTWEPDLSASVEARNDAHILVST
                     AAATNIIADTLCARQDLIDSYPETVRDFVHGWFDGIEMMKSDPAGSYAVVGKALKLDE
                     ETVSGMLSGLKLTPYADNAQFYGLTGGKAHYETLFDTAFVIWRKKGLVTRPVDAKDWA
                     DTRFLGALAAAYQTQQVEEPKVAAKAPAATDRAIINKQIQIHFTPNSDEIMPGSFFVL
                     DALGETMTSFGNTYLRVEGNTDATGKASLNMALSERRALAVKNYVLTNFPNIEASRFQ
                     TIGRGSTNPVADNTTETGRQLNRRTDIKVVLATN"
     gene            complement(5067..5780)
                     /locus_tag="PLP50_10080"
     CDS             complement(5067..5780)
                     /locus_tag="PLP50_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HPA51935.1"
                     /translation="MANVGFFARLGNLWKGFLSIWISDVEKEHPEIAYENAINSMVEK
                     YTKLKTATAAIIRRREDIDERLKKATRELAQTEAELNAAVETAQDDLAVILIQKKNQL
                     EADITEMRADMDTASKDAESAKSSLLQVQGEIKKLKAERDTMLARMQSAQARLKIQEQ
                     LDGLSVDAEVKALENVRDHIKTTVAAANLGKELSETSLDGRLAALKTQAGDVQAKQQL
                     AELKAKRAAAQAAQGQKTM"
     gene            complement(5782..6447)
                     /locus_tag="PLP50_10085"
     CDS             complement(5782..6447)
                     /locus_tag="PLP50_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51936.1"
                     /translation="MSEKKNWGSTVLGWFVVKEAGEEASLPAEVDVPAADGEPAAAPP
                     PGPPPVTFVKEPPRAVAGQVDFDGVLDAAGVDAQERDLFAKAQALLGGLPPGTDPAVK
                     RQIVEASLKAFGVPVDKIIEAGVESIQALEGYLRRTAGETADTAREVQTIVAGYEQKI
                     AEARALLDRKLQEQAAVQTSCNARKLEVQQVLEFFGQEEVARVVHSSPKLIEPTAPPE
                     RKD"
     gene            6531..7493
                     /locus_tag="PLP50_10090"
     CDS             6531..7493
                     /locus_tag="PLP50_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HPA51937.1"
                     /translation="MARGHWARSTEELRKRLATAVPRETLRALHRRSAARHFAVVLRQ
                     SLLFASAFAAGWEFESLWVRVPAAIVLGLCVFDGTILLHEVVHRTVFRSDRPRAYRLL
                     GLLYALPSGLSPSQFTRWHLDHHAELGSATDDPKRAWLSPKRNVRWLKLLYATPALFP
                     IYFRAARHAMADYPAALRRTILLERAAATLLHLAVAGAIGLLGGWERVVWLWAIPVFV
                     VFPPVFTLNRLGQHYDVDPGEPSRWGTRMARSRFWDVAWLWSSYHLEHHYFPTVPFYR
                     LRDLNAALTPFLDGEGLRARRYRELVWDWLVRNRAPHTDWRARL"
     gene            7512..8945
                     /locus_tag="PLP50_10095"
     CDS             7512..8945
                     /locus_tag="PLP50_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA51938.1"
                     /translation="MAENLGRILVVDDEPFARTVVRRMLEVDGWEVSEARSTQEARAL
                     LAGEPEPVALLLDVLLDGESGLEYLERLREEGVLVPVIVMTAMEDVETAVRAMKAGAY
                     DFLVKPVTPERLRTALTHAAERRALLDENRDLRKRVRQEAFGRTLVGESPAMKALFSE
                     MERVVETDIAVCLLGETGTGKELVARWLHENGPRGKGPFVDINCAAIPETLIESELFG
                     HEKGAFTGAAARHKGKLEQADGGTLFLDELGEMAHATQARLLRVLQEKTLVRVGGSET
                     IPYNARLISATQRDLTAAVRQGSFREDLYFRVVVYPLRLPPLRERIEDIALLVHHFIR
                     VFRASTGRPVEGITPEAIVTLEAYTWPGNVRELQNAVFRAVVSARGPFLTTADFPDLS
                     PDRVPRYQPRPYPARPDGPPTPAAGLQKAAMAAPSLVTMEDHERAAIQQALDASNGNV
                     RAAADRLQVARSTLYRRIRALGLTAAS"
     gene            9044..11179
                     /locus_tag="PLP50_10100"
     CDS             9044..11179
                     /locus_tag="PLP50_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HPA51939.1"
                     /translation="MAIEVSTPPLEKLHGLLPDDLVRAYRIAYTSRRIDDKEIQLKRQ
                     NRIFFQISGAGHEAVQVAAAMLMRPGVDWLFPYYRDRALCLGLGVTPLEMLQTAVGAK
                     AGEASAGRQMPSHWGSRRLNIFTTSSPTGSECLPGVGVAEAGRFLERPESRPFLAATS
                     GFAKDEVSVVTIGDGSTSEGEFWEALNAATVRRLPVLFLVEDNKFAISTPVEVNTPGG
                     SISKVVRGYPGLFLAEVDGCDFLASFDALRWAFAYCTERKGPALVHAHVVRPYSHSLS
                     DDDRLYRTEEERKKDAEGDPLVRMEALLLEHGVLDAEKLAELKAEVDAEVNAAADAAI
                     ESPQPEAAEVFAHVISPDVDPTSPAFDTEDAPAFTGTPTTMVDLINAALKDEMRRDGR
                     IVVFGEDVADATRAEVLAECKGKGGVFKVTAGLQKAFGSDRVFNSPLAEATIVGTAIG
                     WAARGLKPVVEIQFFDYIWPAMQQLRDELATVRWRSGGTWKAPVVVRVAIGGYLTGGG
                     PYHSQSGEVTFAHIPGLRVAYPSNALDANGLLRTAIRCDDPVLFLEHKHLYRQTHNKG
                     ANPGPDYMVPFGKAKVVRPGADATVVTYGATVFRSVVAARKIAEDSGKEVEVIDLRTL
                     APYDFDAIATSVGKTSRLLVVHEDWKTHGFGAEVAARASDELFERLDAPVRRVGAKDV
                     FCGYAPQLEDATLPQSAEIEQALRELLAY"
     gene            11181..12107
                     /locus_tag="PLP50_10105"
     CDS             11181..12107
                     /locus_tag="PLP50_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA51940.1"
                     /translation="MKRALPPLALAALFLGALGVGAFFADPIGLVADAGALRFRFAGA
                     RATTVPGPSGTRLFARELGPRSAGTPVVLLHGLGAASHYWSETAIDLFRAGRTVIVPD
                     APGSGRSDPPAAESGWGIPGRVAAVSALLDALGVRKADLVGHSLGGWTAGAFALAEPH
                     RVRRLVLVDAAGFSKPDPRAEAELRASLSPGDRAGARRLYDLLFLRKPFPASGFLLDA
                     FGRQYRSENVVATVAALEEKDGLLGREAELPEGTVFVHGAEDLICPLPDTRAAFARAR
                     NARLFVIRGTGHDAPLEAPRLFHETLRAALAE"
     gene            complement(12123..13967)
                     /locus_tag="PLP50_10110"
     CDS             complement(12123..13967)
                     /locus_tag="PLP50_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51941.1"
                     /translation="MTASARRLATGTALLTLLLAGAALRLHRVRAAPPGPWIDEALAL
                     RAARVVSATNAPLLGTSPLQPPDAGFVNSWLTNLSLHGLSVIDRAAGGGIASVRAMSV
                     LPALVLLAGIAALAGAAAGGRPFPLLLSAFLGATSSWLLVTGRWGWNAVATSAVVVLA
                     AALALLAASRRSVPLGAAAGALAGLAGWGYVAAWALLPLPPALWLVGRLRERRAAAND
                     APDAAAFRRVAAAGLVAFALVLAPLAAHYAAHPERALARARELSAAREGTGTALSSLA
                     RNAAAYARLFALGGDPNERHGDPGRPVLPLAVTGLALVGAAAGLRRGGSERLLASAAG
                     LFLLASLFAVEEAANAYRAVHAAPFLVVLAALGAEHLVAVSGGRRRLAIAAVVAVVGV
                     AGATDAGAFLRWLGSPRLEGAFGGPERALADAIRADLAARGPADVVLSPGAARNAFVV
                     DTLLARPESGRPAIRLADGLDALRYRPSRGLLLAESAATARPAAPGSLAVATGGALPG
                     FPGWRLLRVPAGAAAAAAAASLGALPLLPAPGRGELLVAEEGLYSFSSRGGLDARLDG
                     SVLFGEARPAGRLTVRLAPGRHDLFVHALRPGAELRVTGPDGFVLPAP"
     gene            complement(13964..15649)
                     /locus_tag="PLP50_10115"
     CDS             complement(13964..15649)
                     /locus_tag="PLP50_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPA51942.1"
                     /translation="MSEALRRSHPALLGAIAGLAAGNVLLALNPHLLAPLPSLRLLAA
                     CGLLGFLVLAPFGLLVREPGTPSAGFWGLSAVVFTLLAVHAEAQRKLYYDFLGNGTRR
                     LLVGVAIVGAVSAVASLLAARKRPRGRRAAAYLLGPAALLSAVPLLGRHGPERLPLDP
                     PQSIPRTATRSLLVVGLEGVSWELLSRAASDGSLPVFARLLDEGAGGPLGTLAPYDRS
                     ALWTTAATGKMPRKHGVVSETSQSTPAGLLRLGPRLPGFARPIPLPLASPRREGGATR
                     RSRTFWEVLAERGHEAAVLSWPAADPPREGLVLWATERFFSEEGAKGEALPEETAERA
                     RLLLLGAGNLDRPLARALEPAGLPDAERTQARAAAGAARDLGIVGATLAGVPTGPGSV
                     SALVLSGLSGPARALGPAADPRRYFGVSRRETDAAEKALLAYYRFLDDTLGDLLEREG
                     RERTLCVFAPASFGPAPPLTALLDLLRGRAPVASPDGGDDGFLVLWGSGIRAGVRLTS
                     ADVVDLAPTLLALAGEPIARDMDGRVLAEAFDDRFSQGTSIPVVETFEPEGPQ"
     gene            15682..16488
                     /locus_tag="PLP50_10120"
     CDS             15682..16488
                     /locus_tag="PLP50_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPA51943.1"
                     /translation="MVVNEALRILRTVRYDAAHFSVFLQAGTIAAAAKPGQFVMIQAG
                     DGLRPYLRRAFSVADVTTVAGVPGIELVVRAVGVGTEALGRFAEGTPIPVLGPLGVPF
                     PIEDLKQSDRVALVAGGIGLAPLVFLSRRLAERGIPADLFYGGRNESEVLKRPDFERF
                     LGAHRCRYATDDGSLGKRGRVTDIFGAALEAGTSYRRVFACGPVPMFRTLAGILESAG
                     VEGSFALESEMACGFGVCLGCVLPGRDGRFHTICTEGPCLPPTAIDWSRV"
     gene            16502..17446
                     /locus_tag="PLP50_10125"
     CDS             16502..17446
                     /locus_tag="PLP50_10125"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPA51944.1"
                     /translation="MSVDLTVHLGRLLLKNPIATASGTFGYGLEFAEFVDLASLGAIS
                     VKGLSLRPCLGNPPPRICETDAGMLNAIGLQNVGVEEFLGEKLPKLHEAGATVIANVW
                     GDTEAEYRAVVERIGDDPRVAAVELNVSCPNVVKGGMVFGNDPSALGALVAAVRPATR
                     LPLVVKLSPNVTDVVAAARAAVDGGADVLSLINTLVGLSIDIETREPKIGFTTGGLSG
                     PAIRPVAVRMVWEVRRALPHVPIFGIGGIETAEHVVEFLIAGANAVQVGTASFRDPGI
                     SGRLVRDLARWCERHGVERVSDLTGTLKTRPRPEKAYV"
     gene            17439..18155
                     /gene="pyrF"
                     /locus_tag="PLP50_10130"
     CDS             17439..18155
                     /gene="pyrF"
                     /locus_tag="PLP50_10130"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPA51945.1"
                     /translation="MSDGRKRIAVALDASERSRLLELARLVGPAVGVLKVGLEAFCGH
                     GPELVRELAALAPVFLDLKLHDIPNTVGGAAAAAARTGASILNVHAGGGAEMMRAAGE
                     RAREAATSAGLPVPKVIAVTVLTSLDAAALAEVGFAGAPRETALRLALLARQAGLDGV
                     VCSPEEIGTIRSACGPEFLLVVPGIRPAGSAAGDQKRIATPRAAARAGADLLVIGRPI
                     TGAPDPAAAARAIADEINGA"
     gene            complement(18198..18608)
                     /locus_tag="PLP50_10135"
     CDS             complement(18198..18608)
                     /locus_tag="PLP50_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51946.1"
                     /translation="MAERRKGRKASAGDGLEADFGPGPEPAEEERAEAAGHRPRGPGV
                     EGAPEEGWTAEAGARVPCGKEGCLLKPLGDLATRFVLELARRGHSDSAARAATSGIEL
                     LKALRDFLDEEIALAERAAEARRTAARYTKIPVE"
     gene            complement(18601..>18906)
                     /locus_tag="PLP50_10140"
     CDS             complement(18601..>18906)
                     /locus_tag="PLP50_10140"
                     /inference="COORDINATES: protein motif:HMM:NF037143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51947.1"
                     /translation="AKLGASLYAGLDPAASFRTTPPYRFQKRAGAYVLTLDLPFVEKE
                     DVDLAVASGDLVVTLGNFRHHIPIPRTLAGSVPTKAKVEAGRLTVRFERSAAEAERG"
     assembly_gap    18907..19006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19007..19961)
                     /locus_tag="PLP50_10145"
     CDS             complement(<19007..19961)
                     /locus_tag="PLP50_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HPA51948.1"
                     /translation="MRILLYTGKGGVGKTTVAAATGLALAARGLKTLVLSVDAAHSLA
                     DAFDLDGRLADKRHGVPVAVAGNLWIQEVDVTEEIGRHWSDISGYIATLLAVTGIEEV
                     LAEELAIFPGMEEVSALLYVNRYAREEAYDVLILDCAPTGESLRFVSLPPTLDWYMKK
                     LFRIERSILKVARPMAEKVTNLPIPSERYFDNVKALAGKLDGVDALLKDPKTTTVRLV
                     TNAEKIVLRETQRAFMYFGLYGFTVDAVIVNRLLPDGLGDPFFEKWRRTQATFLAEAK
                     RYFDPVPIWTVPLEDDQVVGAAGLAKLGASLYAGLDPAASFR"
     gene            complement(20012..20401)
                     /locus_tag="PLP50_10150"
     CDS             complement(20012..20401)
                     /locus_tag="PLP50_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPA51949.1"
                     /translation="MADAAGVRLDVFLDVACIFPTRSQAKEACEGGKVDLNGSAAKPH
                     RTVKPGDTLQVTVPGRRRTLVVRAVAERHVPKAEARTLYEETTPELTSEQLEARRLEK
                     LLAPRRDAGAGRLSRREREERGRLRGY"
     gene            complement(20720..21082)
                     /locus_tag="PLP50_10155"
     CDS             complement(20720..21082)
                     /locus_tag="PLP50_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51950.1"
                     /translation="MSTRKRNRGEGAFGAIVGIAIVVVVGIALFKIVPLHYAGNKVKD
                     AMSEQANFAGVKPLDKIQYEIFVIAQEAGTPLTIQDIKIRRHGGDVIVEAKYMQKTSV
                     LGYQYTYAFDHTVQKPVF"
     gene            complement(21116..>21354)
                     /locus_tag="PLP50_10160"
     CDS             complement(21116..>21354)
                     /locus_tag="PLP50_10160"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPA51951.1"
                     /translation="PAETFFALGDNRDESRDSRAWGPVPMGHVRGRALFVYWSVRPPA
                     EPIVGRNAGLRRLLDGAIHFFSRTRWKRTFHAVG"
BASE COUNT         2861 a         7666 c         7769 g         2958 t
ORIGIN      
        1 cgacgaggac gaggtcgtgg acgttcccgt ggaggacgaa gaggaccgcc tcgttgcgca
       61 ggtagcggcg gcggaggtct tcggcccagg cggggaggat gcgggcttcg gccatggcgg
      121 ctccttggtc agaacgagta gcgcgagacc cagcgggtcg gctggaacgg ggggattgag
      181 cgcgggacga ggagggcggc ccccgggcga aggtgggcgc ggaactcccc gggaagcgcc
      241 ttcccggcgt aggccgcgga aaggtagcgc cagtcggcct cggcccagtc cgagagccac
      301 gcgtcgaggt cctcgacgcc cgcctcgtga aagagcgcca gggcgtactc agaggcttcc
      361 cgctcgtagc gtcggatcgc ctcgagccgc tcggggcccg ggctcgcgtc gagcgacgag
      421 gccgcgatcc cgagctcccg gagctcctcc gacacgttcc actggaccgt gtgcccgaag
      481 aggtgggcga ggacgaagag cgcgacgtcg acctcctggt cgtggtcgac ggcaatctcg
      541 acgccgtcga agtcgccggt gttcgggtcg aggacgtcgg cgatcctcac ggggatcccg
      601 tacctcgact cgaggaggtc gtgcaccgcg tcgaacgcgg cgcggaggcg ctccccggag
      661 ggaccgggtc cctcgttcac gggttcgaga ggccggaggc ctcgagcatc gcctcggtgt
      721 cgcgcgccga ctgcttgatc gcctcgaccc cctcgaggag ctcgtcgagc tggcccgaga
      781 gctccttcgg cgactgcatc gtgacgatct ggtcggcgat gagctggaac gtgttctcga
      841 tcaggtcgag ctggccgcgg gcgacggcga ggtagtggtc gatctcccgc atccgctcct
      901 gacgcttcac gacgatcgcg aggttcttct gcgagatctc ggtccgctgg tcctcggggt
      961 ccccccggcg gacctcggcc gcgtagcgct cgacgtcccg ctcgagggcc cgcgcgtcga
     1021 cggagtcgag gtaggagcgg tagcgggcgc aggtgagggc catctcgacg aaggcgtcga
     1081 cgagacggtc cgtcttcacg agctcgcccc ggaggagctc gcccgtgaac gtcgggttct
     1141 gcgcggcgag ccgctcgatc tccctgcggc gggcgacgag cgcctcgacc ctctcccggt
     1201 cgtgctccgg caggaccgcg atccgcttcc tccgttcctc ggcctcgatc gcctcgcgaa
     1261 gcttcgcgaa gcgcgggtcc cagaggaggt ggcggaggcg cttgctgtcg ggggcccaga
     1321 ggagccagag cgcctcgagg ccgagggcgc cgaggatcgg gagcgggttc agcgtcagga
     1381 gcgacgcggc cgccgctccc ccgaggaggg agaggttgta ggggttgagg aacgcggctt
     1441 tcacgtagga cggctgggcg ccgaagaccg tgtcggccgc ccttcccttc agcaggtcga
     1501 ccgcggtctc cttcgcggcc gcgcgcacgg ccggcgagac gcccgaccgg gccgcggacc
     1561 tctgcgccgc ggagcggttc cgggggacct gctgctccgc caccgtgccc cgtcctagcc
     1621 cgccggctgc ggggcgtaga gcatgtcgag gagcttcttc tcgatcgccc ggaactcgtc
     1681 cgtcgacttc aagtggacgt cccgctccgc gaaattcggg acgtcgaccc ggtggaggat
     1741 ccgggccggg cgggaggaga ggacgtagca ggtgtcggcg aggtagaccg cctcggagac
     1801 gtcgtgcgtc acgaagagga tcgtgttgtc ctcgatcctc gagacgtcga ggaggagccc
     1861 ctgcatcccc caccgcgtca tcgggtcgag cgcgccgaac ggctcgtcca tcaggacgac
     1921 gcgcggcttg ttcacgagcg tccgcgcgat ggcgacccgc tgcttcatcc cgcccgagag
     1981 gtccttcggg aaccgcttcg cgaagtcgag gaggccgacc tccttcagga cctcgcgcgc
     2041 gcggtcgccg cgctccttcg cggcgacgcc ccggagcttc aggccgtact cgacgttctc
     2101 ctggacggtg agccagccga acgacgtgta ggcctggaag accatcccgc ggtcctgcga
     2161 ggggcccccg atcggctgcc cgtcgaggag gacctccccc ttcgtcggcg gcaggaggcc
     2221 ggcgacggcc ttcaggatcg tcgacttgcc gcacccggag gggccgagga agacgacgat
     2281 ctccccggcg ccgggcttgt cggcgatgtc gaaggagacg tcgcggacgg cctcggtctt
     2341 ctcgcctccc cgcccgatgt acgtgacgga gaggtccttc accgagagcc gcgggacggc
     2401 ggccggcgcg gcggcgctca cgcgttcacc tcgcgccagc ggaacaggag gcgcgagacc
     2461 gacgagatga ggaagtcggt caggaggccg atgccgccga tcacgagaag gccggcgaac
     2521 gaccaggaag ccttctggtg cgtccgggcg agctcgacca tgtagccgag gccgtgctcg
     2581 gggttcaccg cctcggcgag gatgacgtag gtccaggaga tcgcgttcat gacgcggaag
     2641 gaatcgaaga tctcgggagc cgccgccggg aggaggacgg tccggatcac ctgccctcgc
     2701 gtggcgccga gcgtcatcgc cgtctggacg tactcctcgg ggaccgtgcg gatcgccgtg
     2761 acgacgacgg ggaggaggta gacgaagacg ccgaggaaga ggaacgcgat cttctgcttc
     2821 tcgtagatcc cgaaccagag gatgaggagc gggatgaagg ccgagatcgg catgtaccgg
     2881 agcggggcga cgatcggctc gaagagcctg ttcaccgggg tgaacgcccc catcaggacg
     2941 ccgagcggga gcgcgaccgc cgaggcgatg aggaacgcga gcgcgatccg cctcgtggag
     3001 acgaggatcg ccctccagag gtcgtgctgc aggaagagct ggagcgtgcc gcggacgacc
     3061 tccgtcggcg aggggaggaa gtcggccggc gcgagaccgc cgtagctcag gacgcaccag
     3121 acggcgagga cgagcgccgg catgacgaag ccgaggacga gcgccgtccg cttcgggagc
     3181 ggcgcccgga gcgcgaagag tccacggttg ctcaagccat tcccctcctg cctcgtctcc
     3241 cggcgcggga ggcgcgtcgc cgcgcctccc gggtacccgc cccctatacc ggtctccggt
     3301 gggggacgcg gggggatcga gccccccccg cgatcgtttc agttcgtcgc gaggacgacc
     3361 ttgatgtcgg tccggcggtt cagctgccgt cccgtctcgg tcgtgttgtc ggcgaccggg
     3421 ttcgtcgagc cgcgcccgat cgtctggaac cggctcgcct cgatgttcgg gaagttcgtc
     3481 aggacgtagt tcttcacggc gagcgcccgc cgctcggaga gggccatgtt cagcgaggcc
     3541 ttaccggtcg cgtcggtgtt cccctcgacg cgcaggtacg tgttcccgaa cgaggtcatc
     3601 gtctcgccga gggcgtcgag gacgaagaag gagcccggca tgatctcgtc gctgttcggc
     3661 gtgaagtgga tctggatctg cttgttgatg atcgcccggt ccgtcgccgc cggcgccttc
     3721 gcggcgacct tcggctcctc gacctgctgc gtctggtacg cggcggcgag ggcgccgagg
     3781 aagcgggtgt cggcccagtc cttcgcgtcg accggccgcg tgacgagccc cttcttccgc
     3841 cagatgacga acgccgtgtc gaagagggtc tcgtagtggg ccttgccgcc cgtcaggccg
     3901 tagaactggg cgttgtcggc ataaggggtg agcttgaggc ctgagagcat cccggagacg
     3961 gtctcctcgt cgagcttgag cgccttgccg acgacggcgt aggagcccgc cgggtccgac
     4021 ttcatcatct cgatcccgtc gaaccagccg tggacgaagt cgcggacggt ctccgggtag
     4081 gagtcgatca ggtcctgccg ggcgcagagc gtgtccgcga tgatgttcgt ggcggcggcg
     4141 gtggagacga ggatgtgggc gtcgttccgc gcctcgacgg aggcggagag gtccggctcc
     4201 caggtcacgg cggcgtcgac ctgccgcgcc ttgaacatcg cggccgcggc cggcgcgtcc
     4261 tgcgtgaaga cgatcgcctt ctcgacgccg gcgcgctcct cgggcgtgag gcccgactgc
     4321 gacaggaggt agaggagcag gaagtgggag ggcgtgaact gcgtgcaggc gaccttcttc
     4381 ccgcggaggt cctcgatcga cttgatcgag gcgagcgaga cgatgccgtc gccgccgcgg
     4441 gaccagtcct gcaggaggat cgacttcgcc ttcgccccct gctcggcgag gatcgacgcc
     4501 tcgcgcgccc agttgtcgac ggtgttccac atgacgtcga cgtcgccctt ccggaacgcg
     4561 gcgagcttgg ccgccgggtc ctcgaggagg acgaacttca cgtcgaggcc gtacttccgc
     4621 ttgtagtagg aggtggggtt cgggtccatg ccggcgttga agacgacgcc gggggcatgc
     4681 cccgcccagg tgttgatggc gacgacgagc ggccgtccga gcttgccgga gccggtgagc
     4741 gcggcacccg tcaccccctc ggaacccttg ccccgttcgg cgtcggcggg agcgcccttg
     4801 agcgcgtcga agtcggcgga gtcgactccg gcgctctcga cggctccctt cttctcggct
     4861 cccgcccact gccggacggt gtcccccttg tagtgccaga aggcgtagcc gagaacgccg
     4921 aggacgacca cggtgatgaa gagcttcgcg aacggggtga gcttcatgtc cctctccctg
     4981 gcggaggggc gaccgctccg gcgccggatc gcgccgcggc ccctctccgc gaaaacgcga
     5041 gcggcccccg cgggggccgc tcgtccttac atcgtcttct ggccctgggc cgcctgggcc
     5101 gcggcccgct tcgccttcag ctcggcgagc tgctgcttgg cctgcacgtc gccggcctgg
     5161 gtcttgagcg ccgcgaggcg gccgtcgagc gaggtctccg agagctcctt gccgaggttc
     5221 gcggcggcga cggtcgtctt gatgtggtcg cggacgttct cgagcgcctt cacctcggcg
     5281 tccaccgaga ggccgtcgag ctgctcctgg atcttcagcc gggcctgcgc cgactgcatc
     5341 cgcgcgagca tcgtgtcgcg ctcggccttc agcttcttga tctcgccctg cacctggagg
     5401 agcgagctct tcgccgactc ggcgtccttc gaggccgtgt ccatgtcggc ccgcatctcc
     5461 gtgatgtcgg cctcgagctg gttcttcttc tggatcagga tgacggcgag gtcgtcctgg
     5521 gccgtctcga cggccgcgtt cagctcggcc tcggtctggg ccagctcgcg cgtcgccttc
     5581 ttcaggcgct cgtcgatgtc ctcgcggcgc cggatgatgg ccgccgtcgc cgtcttgagc
     5641 ttcgtgtact tctccaccat cgagttgatg gcgttctcgt aggcgatctc cgggtgctcc
     5701 ttctcgacgt ccgagatcca gatcgagagg aatcccttcc agaggttgcc cagcctcgcg
     5761 aagaaaccga cgttggccat gctaatcctt cctctcgggc ggggcggtcg gctcgatgag
     5821 cttcggggac gaatggacca cccgcgccac ctcctcctgc ccgaagaact cgaggacctg
     5881 ctggacctcg agcttcctcg cgttacagct cgtctgtacg gcggcctgct cctgcagttt
     5941 ccggtcgagg agggcgcgcg cctcggcgat cttctgctcg tacccggcga cgatcgtctg
     6001 gacctcgcgc gccgtgtcgg ccgtctcgcc cgccgtccgg cgaaggtagc cctcgagcgc
     6061 ctggatcgac tcgacgcccg cctcgatgat cttgtcgacg gggacgccga acgccttcag
     6121 cgacgcctcg acgatctgcc tcttcacggc cgggtcggtc cccgggggaa ggcccccgag
     6181 gagcgcctgc gccttcgcga agaggtcgcg ctcctgggcg tcgacccccg cggcgtccag
     6241 gacgccgtcg aagtcgacct ggccggcgac cgccctcggc ggctccttca cgaacgtgac
     6301 gggcggcggt ccgggagggg gcgcggccgc gggttccccg tccgccgccg ggacgtccac
     6361 ctccgcgggg agagaagcct cctcccccgc ctccttgacg acgaaccagc cgaggacggt
     6421 ggagccccag ttcttctttt cgctcatggc aagtctcgaa ttcgagatct tatcaagcgg
     6481 gcggcccgga atccgctccc cgaccgggcc gtcgtaaagt cgggagggag atggcgcgcg
     6541 gtcactgggc ccggtcgacg gaggagctcc ggaaacgtct cgcgacggcg gtgccgcgcg
     6601 agacgctccg ggcgctgcac cggcgttccg ccgcgcggca cttcgcggtc gtccttcgcc
     6661 agtcgctcct cttcgccagc gccttcgccg ccggctggga gttcgagagc ctctgggtcc
     6721 gcgtcccggc cgcgatcgtc ctcgggctct gcgtcttcga cgggacgatc ctcctccacg
     6781 aggtcgtcca ccggacggtc ttccgctccg accggccgcg cgcataccgc ctgctcgggc
     6841 tcctgtacgc cctgccgtcg gggctctcgc cgtcgcagtt cacgcgctgg cacctcgacc
     6901 accacgccga gctcggctcg gcgacggacg acccgaagcg ggcctggctc tcgccgaagc
     6961 ggaacgtccg gtggctgaag ctcctctacg cgacgcccgc cctcttcccg atctacttcc
     7021 gggcggcccg gcacgcgatg gcggactacc ccgccgcgct ccggcggacg atcctcctcg
     7081 agcgggcggc cgcgacgctc ctccacctcg ccgtcgcggg ggcgatcggc ctcctcggcg
     7141 ggtgggagcg ggtcgtctgg ctctgggcga tccccgtctt cgtcgtcttc ccgccggtct
     7201 tcacgctgaa ccggctgggg cagcactacg acgtcgaccc cggcgagccg tcgagatggg
     7261 gcacgcggat ggcgcgctcc cgcttctggg acgtggcctg gctctggtcg agctaccacc
     7321 tcgagcacca ctacttcccg accgtcccct tctacaggct gcgtgacctg aacgccgccc
     7381 tgaccccgtt cctcgacggc gaggggctcc gcgcgcgccg ctaccgcgag ctcgtctggg
     7441 actggctcgt gaggaaccgg gcgcctcaca cggactggcg cgcccgactg taatagactt
     7501 ggaagccctt tatggcggag aacctcggca ggatcctcgt cgtcgacgat gagcctttcg
     7561 cacggacggt cgtccggcgg atgctcgagg tggacggctg ggaggtttcc gaggcccggt
     7621 cgacgcagga agcccgcgcg ctcctcgccg gcgagccgga gccggtcgcg ctcctcctcg
     7681 acgtcctcct cgacggcgag agcggtctgg agtacctcga acggctgcgg gaggaggggg
     7741 tcctcgtccc ggtcatcgtc atgaccgcca tggaggacgt ggagaccgcc gtccgggcga
     7801 tgaaggccgg cgcctacgac ttcctcgtca agccggtcac tccggagcgc ctccggacgg
     7861 ccctgacgca cgccgccgag cggcgcgcgc tgctggacga gaaccgggac ctccgcaaga
     7921 gggtccgcca ggaggcgttc ggccggacgc tcgtcggcga gagccccgcg atgaaggcgc
     7981 tcttctccga gatggagcgg gtcgtcgaga ccgacatcgc cgtctgcctc ctcggggaga
     8041 cggggacggg caaggagctc gtcgcccggt ggctccacga gaacggaccg cgcggcaagg
     8101 gcccgttcgt cgacatcaac tgcgccgcga tccccgagac gctgatcgag agcgagctct
     8161 tcggtcacga gaagggggcg ttcaccggcg ccgcggcccg ccacaaggga aagctcgagc
     8221 aggccgacgg cgggacgctc ttcctcgacg agctcgggga gatggctcac gcgacccagg
     8281 cccggctcct ccgcgtcctc caggagaaga ccctggtgcg ggtgggcggc agcgagacga
     8341 tcccctacaa cgcccggctc atctccgcga cgcagcgcga cctgacggcc gccgtccgtc
     8401 aggggagctt ccgggaggac ctctacttcc gggtcgtcgt ctatccgctg cggcttcccc
     8461 cgctccgaga gcggatcgag gacatcgcgc tcctcgtcca ccacttcatc cgggtcttcc
     8521 gggcgagcac cggccgcccc gtcgagggga tcacgcccga ggcgatcgtc acgctcgagg
     8581 cgtacacctg gccagggaac gtccgggagc tgcagaacgc cgtcttccgc gcggtcgtct
     8641 ccgcgcgggg gccgttcctg acgaccgccg acttcccgga cctctccccc gaccgggtgc
     8701 cgcgctacca gccgcgcccg tatccggccc ggccagacgg ccccccgacc cccgccgccg
     8761 gccttcagaa ggcggcgatg gccgcgccga gcctcgtcac gatggaggac cacgagcggg
     8821 ccgcgatcca gcaggcgctc gacgcctcca acgggaacgt gagggccgcc gcggaccgcc
     8881 tccaggtcgc ccggtcgacg ctctaccggc ggatccgcgc cctcggcctg acggccgcgt
     8941 cctgagcgcc gggcgcccgc gccggcgccc aaacccgctc tgctactctc ccgtcccgtc
     9001 cggggcggcc cgtgggcgcc cactcccgac gccgaggatg acgatggcga tcgaagtctc
     9061 gaccccgccc ctggagaagc tccatggcct ccttccggac gacctcgtcc gggcctaccg
     9121 gatcgcgtac acctcgcgcc ggatcgacga caaggagatc cagctcaagc ggcagaaccg
     9181 gatcttcttc cagatcagcg gggccgggca cgaggcggtc caggtcgcgg cggcgatgct
     9241 catgcggccc ggcgtcgact ggctcttccc gtactaccgg gaccgggccc tctgcctcgg
     9301 tctcggcgtc acgccgctcg agatgctgca aaccgccgtc ggcgcgaagg ccggggaggc
     9361 ctccgccggg cggcagatgc cgtcccactg gggctcccgg cgtctcaaca tcttcacgac
     9421 ctcctccccg accggctccg agtgcctccc cggcgtcggc gtcgccgagg cggggcggtt
     9481 cctcgagcgg cccgagtccc ggcccttcct cgccgccacc tccggcttcg cgaaggacga
     9541 ggtctcggtc gtcacgatcg gcgacggatc gacatccgag ggggagttct gggaggcgct
     9601 gaacgcggcg acggtccgga ggctcccggt cctcttcctc gtcgaggaca acaagttcgc
     9661 gatctcgacg ccggtcgagg tgaacacgcc cggcggctcg atctcgaagg tcgttcgcgg
     9721 ctaccccggc ctcttcctcg ccgaggtcga cgggtgcgac ttcctggcgt ccttcgacgc
     9781 gctgcggtgg gcgttcgcct actgcacgga gcggaagggg cccgccctcg tccacgccca
     9841 cgtcgtccgc ccctactcgc actcgctctc cgacgacgac cggctctacc ggacggagga
     9901 ggagcggaag aaggacgccg aaggcgaccc gctcgtccgg atggaggcgc tcctcctcga
     9961 gcacggcgtc ctggacgcgg agaagctcgc ggagctgaag gccgaggtcg acgccgaggt
    10021 gaacgcggcg gccgacgcgg cgatcgagag cccgcagccg gaggcggcgg aggtcttcgc
    10081 ccacgtcatc tcccccgacg tcgacccgac ctcccccgcg ttcgacaccg aggacgcccc
    10141 cgcgttcacc ggcaccccga cgacgatggt cgacctgatc aacgccgccc tgaaggacga
    10201 gatgcgacgg gacgggcgga tcgtcgtctt cggcgaggac gtcgccgacg cgacgcgcgc
    10261 cgaggtcctc gccgagtgca aggggaaggg gggcgtcttc aaggtgaccg cgggcctcca
    10321 gaaggccttc ggctccgacc gcgtcttcaa ctcccccctc gccgaggcga cgatcgtcgg
    10381 caccgcgatc ggctgggccg cccgcggcct gaagccggtc gtcgagatcc agttcttcga
    10441 ctacatctgg ccggcgatgc agcagctgcg cgacgagctc gcgacggtcc gctggcgctc
    10501 cggcgggacc tggaaagctc ccgtcgtcgt ccgggtcgcg atcggcggct acctgacggg
    10561 gggaggcccc taccattcgc agtcgggcga ggtgacgttc gcgcacatcc cgggcctgcg
    10621 cgtcgcctat ccgtcgaacg ccctcgacgc caacggcctc ctccggaccg ccatccgctg
    10681 cgacgacccg gtcctcttcc tcgagcacaa gcacctctac cgacagacgc acaacaaggg
    10741 ggcgaacccc ggccccgact acatggtccc gttcgggaag gcgaaggtcg tccgccccgg
    10801 cgccgacgcc acggtcgtga cgtacggcgc gaccgtcttc cggagcgtcg tcgcggcgcg
    10861 gaagatcgcc gaggactcgg ggaaggaggt cgaggtgatc gacctgcgga cgctcgcccc
    10921 gtacgacttc gacgccatcg cgacgagcgt cgggaagacg agccggctcc tcgtcgtcca
    10981 cgaggactgg aagacgcacg gcttcggcgc cgaggtcgcg gcgcgggcgt cggacgagct
    11041 cttcgagcgg ctcgacgctc cggtgcggcg cgtcggggcg aaggacgtct tctgcggcta
    11101 cgcgccgcag ctggaggacg cgacgctccc gcagagcgcg gagatcgagc aggcgctccg
    11161 ggagctcctc gcgtactgac gtgaagcggg cgcttccccc gctcgccctc gcggccctct
    11221 tcctcggcgc gctcggggtg ggcgccttct tcgccgaccc gatcggcctc gtggccgacg
    11281 ccggggcgct tcgcttccgc ttcgccgggg cgcgcgcgac gacggtgccc ggcccctcgg
    11341 ggacgcgcct cttcgcccgg gagctcgggc cccgctcggc cggaacgccg gtcgtcctcc
    11401 ttcacggcct cggcgccgcc tcgcactact ggtccgagac ggcgatcgac ctcttccgcg
    11461 ccgggcggac cgtgatcgtc cccgacgccc cgggctccgg gcggagcgac ccgcccgcgg
    11521 cggagtcggg ctgggggatc ccggggcgcg tcgcggcggt ctcggcgctg ctcgacgccc
    11581 tgggggtccg gaaggcggac ctcgtcgggc actcgctcgg gggctggacc gccggggcct
    11641 tcgctctcgc cgagccgcac cgcgtccgcc ggctcgtcct cgtcgacgcc gccggcttct
    11701 cgaagcccga cccgcgggcc gaggccgagc tccgggcgag cctctcgccc ggagaccgcg
    11761 ccggcgcccg ccggctctac gacctcctct tcctgcggaa gccgtttccc gcctccgggt
    11821 tcctcctcga cgccttcggg cgacagtacc ggagcgagaa cgtcgtcgcg acggtggcgg
    11881 cgctggagga gaaggacggc ctcctcggcc gcgaggcgga gctccccgag gggaccgtct
    11941 tcgtccatgg cgccgaggac ctcatctgcc ccctgcccga cacgcgcgcg gcgttcgcgc
    12001 gtgcgaggaa cgcccgcctc ttcgtgatcc gcgggaccgg gcacgacgcg ccgctcgaag
    12061 cgccccgcct cttccacgag acgctccggg ccgccctcgc ggagtaggag cggcgcggcg
    12121 ggtcagggcg ccgggaggac gaagccgtcc ggcccggtca cgcgcagctc cgccccgggg
    12181 cgcagggcgt gcacgaagag gtcgtgccgt cccggcgcca ggcgcacggt gagcctcccg
    12241 gccggacgcg cctcgccgaa gaggacgctc ccgtcgaggc gcgcgtcgag cccgccgcgg
    12301 ctcgagaacg agtagagccc ttcctcggcg acgaggagct cgccccgtcc gggggccggg
    12361 aggagcggga gcgcgccgag ggacgcggcc gccgccgcgg ccgccgcgcc ggccgggacg
    12421 cggaggagcc tccagccggg gaagcccggc agcgctccgc ccgtggcgac ggcgagggag
    12481 cccggcgcgg cggggcgcgc ggtggcggcg gactccgcga ggaggaggcc gcgggacggg
    12541 cggtagcgga gcgcgtcgag gccgtccgcc agccggatcg ccggtcgccc gctctccggc
    12601 cgggcgagga gagtgtcgac gacgaaggcg ttccgcgcgg cgccgggcga aaggacgacg
    12661 tccgccgggc cgcgggcggc gaggtccgcc cggatcgcgt cggccagcgc gcgctccggc
    12721 ccgccgaagg cgccctcgag ccgcggcgag ccgagccagc ggaggaaggc cccggcgtcg
    12781 gtcgcgccgg cgacgccgac gaccgcgacg acggcggcga tggcgaggcg gcgccgccct
    12841 ccggacacgg cgacgagatg ctcggcgccg agggccgcga ggacgacgag gaacggagcg
    12901 gcgtggacgg cccggtacgc attcgcggcc tcctcgacgg cgaagaggct cgcgaggagg
    12961 aagagccccg cggccgacgc gaggagccgt tccgaccctc cgcggcgcaa cccggccgcg
    13021 gccccgacga gggcgaggcc cgtcacagcg agcggcagga cgggacggcc ggggtcgccg
    13081 tggcgctcgt tcggatcgcc tccgagggcg aagagccgcg cgtaggccgc ggcgttccgg
    13141 gccaggctcg acagggcggt cccggtccct tcgcgcgcgg cggagagctc gcgggcacgc
    13201 gcgagcgccc tttcgggatg cgcggcgtag tgggccgcga gcggggcgag gacgagggcg
    13261 aacgcgacga ggccggccgc ggcgacccgg cggaacgcgg ccgcgtccgg ggcgtcgttc
    13321 gcggcggccc ggcgctcgcg gagccggccg acgagccaga gcgccggggg aagcggcagg
    13381 agggcccagg ccgcgacgta gccccacccc gcgagcccgg ccagcgcccc cgcggcggcg
    13441 ccgaggggga ccgaccgccg gctcgcggcg agaagcgcga gcgccgcggc gaggacgacg
    13501 acggccgagg tcgcgaccgc gttccagccc cacctcccgg tgacgaggag ccaggacgag
    13561 gtggcgccga ggaaggcgga gaggaggagg gggaacggcc gcccgcccgc cgcggctccg
    13621 gccagggcgg cgatccccgc gaggaggacg agcgccggca ggaccgacat cgcgcggacg
    13681 gaggcgattc ctcccccggc ggcgcggtcg atgacggaga ggccgtggag cgagaggttc
    13741 gtcagccagg agttgacgaa acccgcatcg gggggctgca gaggcgaggt gccgagaagg
    13801 ggcgcgttcg tcgccgagac gacccgcgcg gcgcgcaggg cgagcgcctc gtcgatccag
    13861 ggacccggcg gcgccgcccg gacgcggtgg aggcggagcg cggcgccggc gaggaggagc
    13921 gtcaggagcg ccgtccccgt cgcgagtcgc cgcgccgacg ccgtcactgc ggaccttccg
    13981 gctcgaacgt ctccacgacc ggaatgctcg tcccctgcga gaagcggtcg tcgaacgcct
    14041 cggcgaggac ccggccgtcc atgtcgcggg cgatcggctc gcccgcgagg gcgaggagcg
    14101 tcggcgccag gtcgacgacg tcggccgagg tgagccggac gcccgcgcgg atcccggagc
    14161 cccagaggac gaggaagccg tcgtcgcccc cgtcgggcga cgcgaccgga gcccgcccgc
    14221 gcaggaggtc gaggagcgcg gtcaggggcg gcgcggggcc gaagctggcc ggggcgaaga
    14281 cgcacagcgt ccgctcgcgg ccctcccgct cgaggaggtc tccgagcgtg tcgtccagga
    14341 acctgtagta ggcgaggagc gccttctcgg cggcatcggt ctcgcggcgc gagacaccga
    14401 agtagcggcg ggggtccgcc gccgggccga gggcgcgcgc cggaccggag aggcccgata
    14461 ggacgagggc cgagacgctt ccggggccgg tcggaacgcc cgccagcgtg gcgccgacga
    14521 tcccgagatc gcgcgccgcg ccggccgccg cgcgcgcctg ggtccgctcg gcgtccggca
    14581 gcccggccgg ctcgagggct cgcgcgagcg ggcggtcgag gttcccggcg ccgaggagga
    14641 gaagcctcgc ccgctccgcg gtctcctccg ggagagcctc ccccttcgcc ccctcttccg
    14701 agaagaaccg ttcggtggcc cagaggacga gcccctcccg cggagggtcg gctgcgggcc
    14761 aggagaggac cgccgcctcg tgaccgcgct ccgcgagcac ctcccagaaa gtccggctcc
    14821 gccgcgtcgc cccgccctcg cggcgcgggc tcgcgagcgg cagcggaatc ggtcgggcga
    14881 agccgggtag gcgcggcccg agccgaagga gccctgcagg cgtcgactgc gacgtctccg
    14941 agacgacgcc gtgcttgcgc ggcatcttcc cggtcgcggc cgtcgtccag agggccgagc
    15001 ggtcgtaggg ggcgagcgtg ccgagcggac cgcccgcccc ttcgtcgagg agccgtgcaa
    15061 agacggggag actgccgtcg gacgccgcgc gagagaggag ctcccaggag accccttcga
    15121 ggccgacgac gaggaggctc cgcgtcgccg tcctcgggat cgactgcggc gggtcgaggg
    15181 gcaggcgctc gggcccgtga cggccgagca gcggcaccgc cgagaggagc gccgccgggc
    15241 cgaggaggta cgcggcggcg cgccgtccgc gcggccgctt ccgggccgca aggagagagg
    15301 cgacggcgga gacggcgccg acgatcgcga cgccgacgag gaggcgccgg gtcccgttcc
    15361 cgaggaagtc gtagtagagc ttccgctggg cctcggcgtg gacggcgagg agggtgaaga
    15421 cgaccgcgga gaggccccag aacccggcgg acggggttcc cggctcccgg acgagaaggc
    15481 cgaacggggc gaggacgagg aacccgagga ggccgcaggc cgcgaggagg cggagggacg
    15541 ggagcggggc caggaggtgc gggttcaggg ccagcaggac gttccccgcc gcgaggcccg
    15601 cgatggcccc gaggagcgcg gggtggctgc gccggagagc ctcgctcata atccgcgtcc
    15661 gcgagtctcg ccgtcttccg catggtggtc aacgaagccc tccgcatcct ccgcaccgtc
    15721 cgctacgacg cggcccattt ctccgtgttt ctgcaggccg ggacgatcgc cgcggccgcg
    15781 aagccggggc agttcgtcat gatccaggcc ggcgacggcc tccggcccta cctccgccgc
    15841 gcgttctccg tcgccgacgt cacgacggtg gcgggcgtcc ccggcatcga gctcgtcgtg
    15901 agggccgtcg gggtcgggac ggaggcgctg ggccgattcg cggaggggac accgatcccg
    15961 gtcctcggac cgctcggcgt gccgttcccg atcgaagacc tgaagcagtc cgaccgggtc
    16021 gcgctcgtgg ccggcgggat cggcctcgcc ccgctcgtct tcctctcgcg gcgcctcgcc
    16081 gaaagaggga tcccggccga cctcttctac ggcggccgta acgagagcga ggtgctgaag
    16141 cgtcccgact tcgagcgctt cctcggcgcg caccgctgcc gctacgccac cgacgacggc
    16201 tcgctcggga agaggggtcg cgtgaccgac atcttcggcg ccgccctcga ggccgggacg
    16261 agctatcggc gcgtcttcgc ctgcgggccg gtcccgatgt tccggactct cgccgggatt
    16321 ctcgagtccg caggcgtcga ggggtcgttc gccctcgagt cggagatggc gtgcgggttc
    16381 ggcgtctgcc tcggctgcgt cctgccgggc cgcgacgggc gcttccacac gatctgcacc
    16441 gagggaccct gcctcccgcc gaccgcgatc gactggagcc gggtatgaga ggagcgtcgc
    16501 gatgagcgtc gacctcaccg tccacctcgg ccgactcctg ctgaagaacc cgatcgcgac
    16561 ggcgtccggg acgttcgggt acgggctgga gttcgcggag ttcgtcgacc tcgcgtcgct
    16621 cggggcgatc tccgtcaagg gcctctcgct ccggccctgc ctcggcaacc cgccgccgcg
    16681 catctgcgag acagacgccg ggatgctgaa cgcaatcggc ctccagaacg tcggcgtcga
    16741 ggagttcctc ggcgagaagc tcccgaagct ccacgaggcg ggagcgaccg tcatcgcgaa
    16801 cgtctggggc gacacggagg cggagtaccg cgccgtcgtc gagcggatcg gcgacgaccc
    16861 acgggtcgcc gccgtcgagc tgaacgtctc ctgcccgaac gtcgtcaagg gggggatggt
    16921 cttcgggaac gacccgtcgg ccctcggcgc gctcgtcgcc gcggtccgcc ccgcgacgcg
    16981 cctcccgctc gtcgtgaagc tctcgccgaa cgtcaccgac gtcgttgccg ccgcccgggc
    17041 ggcggtcgac ggcggggccg acgtcctttc gctcatcaac acgctcgtcg gcctctcgat
    17101 cgacatcgag acgcgcgagc cgaagatcgg cttcaccacg ggcggtctct ccgggcccgc
    17161 gatccggccg gtcgccgtcc ggatggtctg ggaggtgcga cgggccctac ctcacgtccc
    17221 gatcttcggc atcggcggga tcgagacggc cgagcacgtc gtcgagttcc tcatcgcggg
    17281 ggcgaatgcc gtccaggtcg gaacggccag cttccgcgac ccgggcatct ccggccggct
    17341 cgtccgcgac ctcgcgcgct ggtgcgagcg gcacggcgtg gagagggtct ccgacctgac
    17401 cggcacgctg aagacgcgcc cgcgccccga gaaggcgtat gtctgacggc cggaagcgga
    17461 tcgcggtcgc cctggacgct tccgagagga gtcggctcct cgagctcgcg cggctcgtcg
    17521 ggccggcggt cggcgtcctg aaggtcgggc tcgaggcctt ctgcggccac ggtcccgagc
    17581 tcgtccgcga gctggccgcg ctcgcacccg tctttctcga cctgaagctc cacgacatcc
    17641 cgaacaccgt gggcggcgcc gccgcggccg ccgcgcggac gggcgcctcg atcctgaacg
    17701 tccatgcggg cggcggcgcc gagatgatgc gcgccgccgg cgagcgggcc cgggaggccg
    17761 cgacgtccgc cggcctcccg gtgccgaagg tcatcgccgt caccgtcctc acttcgctcg
    17821 acgcagcggc gctggccgag gtcggattcg ccggggcccc gcgggagacc gcccttcgcc
    17881 tcgcgctcct cgcgcggcag gcgggtctcg acggggtcgt ctgctctccg gaggagatcg
    17941 ggacgatccg ctcggcctgc ggcccggagt tcctcctcgt cgtcccgggg atccgccccg
    18001 cggggagcgc cgccggcgac cagaagcgga tcgcgacacc ccgcgcggcg gcgcgcgcgg
    18061 gagccgacct cctcgtcatc gggcggccga tcacgggagc gcccgacccg gccgcggcgg
    18121 cgcgggcgat cgcggacgag atcaacgggg cctgaaccgc caggacctcc cggtgccggc
    18181 gggaggcgcg cccgcgccta ctcgactggg atcttcgtgt agcgggccgc cgtccggcgg
    18241 gcctcggccg cccgctcggc gagggcgatc tcctcgtcga ggaagtcccg gagcgccttc
    18301 agcagctcga tcccggaggt cgccgcccgg gccgccgagt cggagtgccc ccggcgggcc
    18361 agctcgagga cgaatcgggt cgcgaggtct ccgagcggtt tcagcaggca gccttccttc
    18421 ccgcacggga cccgcgcgcc cgcctcggcg gtccaccctt cctcgggcgc gccctcgacg
    18481 cccggcccgc gaggccggtg gcccgccgcc tcggcccgct cctcctcggc aggctcgggc
    18541 ccgggaccga agtcggcctc gagaccgtct cccgcgctcg ccttccgccc cttgcgtctc
    18601 tcagccacgt tccgcctccg ccgcgctccg ctcgaagcgg accgtcagcc gccccgcctc
    18661 caccttagcc ttcgtcggga cgctccccgc gagcgtcctc gggatcggga tgtggtggcg
    18721 aaagttcccg agggtgacga cgaggtctcc cgacgcgacc gcgaggtcga cgtcctcctt
    18781 ctcgacgaac ggcaggtcga gcgtcaggac gtaggccccc gcccgcttct ggaaccggta
    18841 gggcggggtc gtccggaacg acgcggccgg atcgagcccc gcgtagaggg aggcgccgag
    18901 cttcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccg gaacgacgcg
    19021 gccggatcga gccccgcgta gagggaggcg ccgagcttcg cgaggccggc cgccccgacg
    19081 acctggtcgt cctcgagcgg aacggtccag atcggcaccg gatcgaagta gcgcttcgct
    19141 tccgcgagga aggtcgcctg cgtccgccgc cacttctcga agaacgggtc cccgagcccg
    19201 tccgggagga ggcggttcac gatgacggcg tcgaccgtga agccgtagag cccgaagtac
    19261 atgaacgccc gctgcgtctc gcgcaggacg atcttctcgg cgttcgtgac gaggcgcacc
    19321 gtcgtcgtct tcgggtcctt caggagcgcg tcgacgccgt ccagcttccc ggcgagcgcc
    19381 ttcacgttgt cgaagtagcg ctcggagggg atcgggaggt tcgtcacctt ttccgccatc
    19441 ggccgggcca ccttcaggat cgacctctcg atccggaaga gcttcttcat gtaccagtcg
    19501 agcgtcggag ggagcgagac gaaccggagc gactcccccg tcggcgcgca gtcgaggatc
    19561 aggacgtcgt acgcttcctc gcgggcgtac cggttgacgt agaggagggc ggagacctcc
    19621 tccatgccgg ggaagatcgc gagctcctcg gcgaggacct cttcgatgcc ggtgaccgcg
    19681 aggagggtcg cgatgtatcc cgagatgtcg gaccagtggc ggccgatctc ctcggtgacg
    19741 tcgacctcct gtatccagag gttcccggcg accgcgaccg ggacgccgtg ccgcttgtcg
    19801 gcgaggcgtc cgtcgaggtc gaaggcgtcg gccagcgagt gcgccgcgtc gaccgacagg
    19861 acgagcgtct tcaggccgcg cgcggcgagc gcgagccccg tggccgcggc gacggtcgtc
    19921 ttgccgacgc cgcccttgcc cgtgtagagg aggattctca tcgaatggga ggaggcatct
    19981 tagcccgcga cgccgcgccg gcggcgcgcg gtcagtagcc gcggagccgg ccgcgttctt
    20041 cccgctcccg ccgggagagc cggccggccc cggcgtcgcg gcgaggcgcg aggagctttt
    20101 ccagccgccg cgcctcgagc tgctccgacg tgagctcggg ggtcgtctcc tcgtagagcg
    20161 ttcgcgcctc ggccttcggg acgtgccgtt cggcgacggc cctcacgacg agcgtcctcc
    20221 gtcgcccggg gacggtgacc tggagcgtgt cccccggctt caccgtccgg tgcggcttcg
    20281 ccgccgaccc attcagatcg accttccccc cctcgcacgc ctccttggcc tgcgagcggg
    20341 tcgggaagat gcacgcgacg tcgaggaaga cgtcgaggcg gacgccagcg gcgtccgcca
    20401 ccggctccgg gcggctcctc tcccgcgtca tccccggccc ggcgcgcgga cgagcccccg
    20461 cgccgcggtc ttccgctccc ggtggggggc gcagggggat cgaggctggc cggggggcgc
    20521 cacccggggg gctgagcttc gcgcgccccc cggaccccca cccgacttcg cgcggttcgc
    20581 attcctcgtg cgtccgcgtt cgcacgcccc ccggaccccc cgcctcgcat ccgcgtcatc
    20641 ggcgcagcaa ggcccccgcc ccgcgttcac tcgcccccgg tggggggcgc ggggggaccg
    20701 agaaaccccc gcgttcactt cagaagaccg gcttctggac ggtgtggtcg aaagcgtagg
    20761 tgtactggta gccgaggacg gaggtcttct gcatgtactt cgcctcgacg atgacgtcgc
    20821 ccccgtgcct tcggatcttg atgtcctgga tcgtgagggg agtcccggcc tcctgggcga
    20881 tgacgaagat ctcgtactgg atcttgtcga gcggcttgac ccccgcgaag ttcgcctgct
    20941 cgctcatcgc gtccttcact ttgttgccgg cgtagtgcag cggcacgatc ttgaagagcg
    21001 cgatgccgac aacgacgacg atcgcgatcc cgacgatcgc cccgaaggcc ccttcaccgc
    21061 gattccgttt ccgggtcgac atgctctgcc tcctcatcgg gccctcccgc gcggctcagc
    21121 cgacggcgtg gaaggtccgc ttccagcggg ttctcgagaa aaagtgtatc gcgccgtcga
    21181 ggagacggcg caggccggcg tttcgtccga cgatcggctc cgcggggggc cggacggacc
    21241 agtagacgaa gagggcccgc ccccggacgt gccccatcgg cacggggccc caggcccgcg
    21301 agtcccggga ctcgtcgcgg ttgtcgccga gggcgaagaa cgtctccgcc ggga
//
LOCUS       DAOONL010000059        21286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2431_length_21286_cov_22.201262, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000059 DAOONL010000000
VERSION     DAOONL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21286
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_21286_cov_22.201262"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLP50_10165"
     CDS             <1..680
                     /locus_tag="PLP50_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPA51952.1"
                     /translation="EGRTAQLKIDKAERELAALELKAPHDGIVVLKRNWRGELPQVGQ
                     VVWSTQTLAEIPDLGKLEAEVWVLEADAGGLAPGRPATLSLEGEAGRPVPAKVKSVDA
                     LARPRLRNVPIQYFGAVLSLDAIDPARMKPGQRVAATLDLGGEADALVVPRQALFERG
                     GETVAFRLGKGAFEPVPVTLGATALGRVAVASGLKAGDVVALVDPEAAPEPATSPAPG
                     SPVGGAS"
     gene            677..1912
                     /locus_tag="PLP50_10170"
     CDS             677..1912
                     /locus_tag="PLP50_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA51953.1"
                     /translation="MSLRESLLRALASLSAHKLRSVLTMLGMIFGVGAVIAMLSIGAG
                     AERQALDMIRRMGISNVLVRAKEFRPDELQEVRKKSIGVSLRDAAGIEEAVPGVDFTG
                     PRVTVNPWSVRGRGGKSEARVSGVSWRHAELVSLPLAAGRFLTKEDEETHAQVCVLGD
                     DARRDLFGWEPALGRTVKVNDVWLTVVGVLAPSGEGGDSFQGVSVGSTARELFVPFTT
                     VLRKLDRDALESPLSELVVKLRDGTPATEAARLVGALLDRLHGGAADFEVVVPEALLA
                     QSRKTQRLFNVVMGCIAGISLLVGGIGIMNIMLATVLERTREIGIRRAIGARRKDVLV
                     QFVVEAFALSALGGLLGVGLGVLIAKVVAASAGWPTVVTLWSLLLSTGVSGAVGLLSG
                     FYPALRAASIDPIEALRYE"
     gene            complement(1920..2660)
                     /locus_tag="PLP50_10175"
     CDS             complement(1920..2660)
                     /locus_tag="PLP50_10175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPA51954.1"
                     /translation="MTGSAGADAAPAPRVPDRVLFDDGSVLALDKPAGVSMATSSRPG
                     KSEEEVVSRLLASCGLPETAPRPLLVHRLDEGTSGVVLLARDAATHRRLSEAFQKRAT
                     EKTYRALVWGHPVPVRGETRAPLGRDRKDGRKMRVREEGKPAVTRWTTLRRYASVADL
                     EVAPETGRTHQIRVHLAARGHPLVGDDLYGGGTRWRGVRDPALRAAFAAVAHPLLHAA
                     RVVVPALGIDVEAPLPGDYAALLGLLAR"
     gene            complement(2657..4432)
                     /locus_tag="PLP50_10180"
     CDS             complement(2657..4432)
                     /locus_tag="PLP50_10180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA51955.1"
                     /translation="MLIRMVRRTCVLAAALLWAIPVPRTAEGQEPAAIRVERLPASLA
                     GTWEFALGDPPGGLADLDTLPFRPIAVPGSWQSAGFSGHGTGWYRVTLDLDPSLSIVP
                     LAFTCTQIRDADEVYLDGQPIGRTGGFPPAYDKGTVVERIYELPPSRSSVPGRHVLAV
                     RVYNAGPRGGGITGTPRLEAVSAAFHARTMREAPRALLAAAFGALGLFSLFFYLRDRG
                     QSDFLFFFLTTSVTAVLIVSWLSVWAVFGLPLTFLFRVGHAGVFVLPALAWVLFLKFF
                     ERPIGSLHRVLLSAQVVGALACLLWPRPDDIYYTFPLGGLLGAAASADVLAHLGTAAR
                     RRVRHARSLLAAALVAVLAAAFDVAGLLALGLGASIEIALSGPAFFLLMSVLLAALAD
                     RLARLRLAASTDPLTSLANRAVLFDRIGLELARARRSAHPVALAILDLDGFKGFNDRH
                     GHLTGDRLLVAVAAAITQAIRDTDLAARFGGDEFVVLLPEADATRAVSCLERIRDGVA
                     RARVSGARDGTTVSAGVAVFDPNVRASVSVSAWLRQADTALYAAKAAGRNRVLVAHGA
                     PPGATSAERVPSDDASIRRVPGTPP"
     gene            4513..5223
                     /locus_tag="PLP50_10185"
     CDS             4513..5223
                     /locus_tag="PLP50_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA51956.1"
                     /translation="MELRDSVALVTGAGRGIGRAVALAFAREGARVALMGLTASHLAS
                     VEKEAGALGARCAVLPGDVSDEGSVARCVAAAEQQLGPVDVLVNNAGVWAHGPVDRLD
                     ALVFDHTLAVNLRGPFLMARAVLPGMKSRRRGHVVNVASTAALRGFAGGAAYCASKFG
                     LAGLSQAMLQDVRGSDVRVTCLMPSTVATDMALRGDFLKSPETAIRPEDVAQAVVDLV
                     RLDSRVLPVSLELWQTNP"
     gene            complement(5246..6784)
                     /locus_tag="PLP50_10190"
     CDS             complement(5246..6784)
                     /locus_tag="PLP50_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPA51957.1"
                     /translation="MSGEDLTASRVRRMLPSVSEVLRELTARAPVEPDVAFRAARDVC
                     AEELKRIRDAGGESVPIEVLVERALARVAGVPPPLVVPAPPPLVPAASFEPEPPSPPS
                     GDDPFSETTGALDLRWDREAREPLAAPAVAVPLEAEPPPPLPADEVPVSAPEAEPVDS
                     VAGGPFTVPEEPAGRAPEGGGFVFALPEDEAAPAPPDFPAEPQEEIGDETLTRLSREA
                     ESIDLRSVLAGGGAAAGPPEREEEFGAAATAAVLDAPPGADEPVPPPFEDDEDTVLTI
                     PRGGRRGATREGGPRWGRLAGIAAALAALAALGYLLVGLWIDRGADSGRVAVARQAPE
                     RTEAPAREASEREALPVESPTPEPAVPAAPGAEGATAPASVAVPVPTAPRERPGAVPT
                     VAPALVAPMPVPVKTAPPAPVRQSRDGLVVTRDRAGRPEVFSIHFTSYRDRGAAERDL
                     KRIEALVGREGYVAEVDLGEKGIWFRVMVGEFPSAEEAKGVRAELAAKGTRDMGWVYR
                     VVAP"
     gene            complement(6781..7797)
                     /locus_tag="PLP50_10195"
     CDS             complement(6781..7797)
                     /locus_tag="PLP50_10195"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPA51958.1"
                     /translation="MSGKKDVLHVGIDLGTSRSAICASNGERHVVESWVGWPVDMVAR
                     KVVKKPVVFGHEALENRPMLDLHRPLERGLIKEGSPKAEAAVRELLAHLIEKAGGAGK
                     TVQAVVGVPAEAMRVSRQHLRNAVKGVADALLLVSEPFAVAYGMDALLHTMIIDIGAG
                     TSDFCVMNGRYPTDDDQRTLTHAGDTVDEQLGLQIRARHPDANFSIHMVREWKERNSF
                     VGEPKEPVFVEAPVRGKPTRLEVTAEMRKACESLVGPLAETMLDLLSRVDPEYQQRVR
                     NSVVLAGGGSQIPGLAAALERALEEVGGGRVRSVEDPVFAGASGSLAIAADAPEGDWE
                     RLAG"
     gene            complement(7794..8807)
                     /locus_tag="PLP50_10200"
     CDS             complement(7794..8807)
                     /locus_tag="PLP50_10200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPA51959.1"
                     /translation="MRDAERLKKQMLRDAVRENLDVIEGRPPARARPGGRWVGRAVRG
                     AALVAIPLALFASINAFSGGGVTPTEVVVVPQRTVAPSPEPGLETLDWPAPKSIDPTL
                     FHLDVRTIVLDAGHGGVDPGALTADGLPEKDVTLDVARRVREKLRGGLFRVVMTREGD
                     ETVSLRERSRRARDAGGDVLVSIHVNSIPNPDRRGVETYYLGPTDDPAIRRLASAENL
                     SSGYSLSDFRGLLEEVYVGVRQAESRRLAESVQAELVASTRKVTPEAGDRGVKSAPFV
                     VLIASEMPGILAEVSSVSHAEDARLLRDEAYREAIAEALARGVRAYADARNLAGAGRS
                     GNG"
     gene            complement(8863..9477)
                     /locus_tag="PLP50_10205"
     CDS             complement(8863..9477)
                     /locus_tag="PLP50_10205"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPA51960.1"
                     /translation="MARIYDLSPVLSPRIAVWPGDVPFSREVTTPPDGTTFSSIRTTL
                     HLGSHADAPLHMVAGAAAIDEVPLETWIGTCQVVEARVSPRGRVRLEDLAEPPSAPRV
                     LLKTGTFGSRERFDDAFAGLDPELAPALAARGVVLVGIDTPSVDPFDDHAYTAHRALV
                     AAGVCWLEGLVLADVPPGRYELSALPLRIEGGDGSPVRAVLVAP"
     gene            complement(9485..10348)
                     /locus_tag="PLP50_10210"
     CDS             complement(9485..10348)
                     /locus_tag="PLP50_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HPA51961.1"
                     /translation="MSEATRKIDEAARGASPHAGGSEELVPLDPDHPGFKDPVYRARR
                     NAIARLALEYRDGQPLPDVEYTEDEQAVWRTVWEHLAPLHERFACREWKESSRALALD
                     RGCVPQLSEVNEALKRGAGFQMLPVAGLVQGRTFLGMMGKGVFLSTQYMRHHSVPLYT
                     PEPDVVHELVGHAASLFHPGIVALSRRFGEAAWRVDDAALARLEYVYWYTLEFGLVEE
                     EGQRKAVGAGLLSSFGELGSFESNAEIRPLDCDAAAAIPYDPTRYQELLFVSPSFDRM
                     VEDVSAWLERQ"
     gene            complement(10455..11993)
                     /locus_tag="PLP50_10215"
     CDS             complement(10455..11993)
                     /locus_tag="PLP50_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51962.1"
                     /translation="MRGGRASVRAAAIATVLLLGAAFIGRSVVSWDAAEAISDTLGFL
                     VQGRFEAAKLPSAELGPDSAFAFRSHYGLFPTLLLLPFVAVPWALRSVLGAQGLDAGI
                     ALAWTTGTLLAVAAFRRLARFLVPESSPWWGPAFLASTFLWAYAADSFFETFAAAGLA
                     CAAAEILEGGGRTGLRPAFLAAAAFSGAALLKPVLWILVPVFVLGAALAWRGRAGGLR
                     RTFVFGALVACGLAVSWTASALRFGGGENFGYGDEALRFTTPLLQGLYGLLLSPGRGL
                     LFFAPFAVVGAARLLFRGVPLAVRVLCGVAPLLLVLTVARFEGWHGGSAWGPRHTLPV
                     LPLLAAPALLARRRAVLPAVAAGVGVNALGVFVAAGAWMSWVELSRPPEGSSWPRSGP
                     EIVSVVPSLSPISGHAFLLGRSLGLSLASPVSRAGATERVPAPGAGQFISPLFLRALF
                     GLPSMHPIVPALLHRIGAAYVLRGDPAAAAPFLREALALAPARPGLRELLGEVEARTQ
                     PHPR"
     gene            complement(12002..12202)
                     /locus_tag="PLP50_10220"
     CDS             complement(12002..12202)
                     /locus_tag="PLP50_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51963.1"
                     /translation="MAKQRSDDEQKAVETILRALAHRLQRLRDEAAMQALRGETDPGV
                     EKGLDMALRELNDLLPRRKRVR"
     gene            12251..12967
                     /locus_tag="PLP50_10225"
     CDS             12251..12967
                     /locus_tag="PLP50_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPA51964.1"
                     /translation="MSVDVLLVRTHSPGNLGSAARACKAFGAGLLLLDPRADAAHEDA
                     RAFASGAEDLLDAAPRLASWEEAEDGAGAILALTSLRGRATRGLPPAWSWAAARRTAG
                     SGRLLLVFGPERSGLTTEEVRRSQGRLALPTRPGFPVLNLAQAVAAALALLEGGRSAR
                     GGAEAGEPAGRDAASAEAHRRLREELATALRASGFLGREDDAVLFELLAPLLRARPTG
                     REAELWTGALRKLGRRVRGA"
     gene            complement(13002..16301)
                     /locus_tag="PLP50_10230"
     CDS             complement(13002..16301)
                     /locus_tag="PLP50_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPA51965.1"
                     /translation="MRTLPILLALSLVAPAGLAAPAKAAAKDSKAAAEKPKEPYSAET
                     FAGLALRDLGPALTSGRIGDLAVDPVRKGTYYVVVASGGVWKTTNWGTTFEPVFDSEG
                     SFSIGCIALDPTNPLVVWVGTGENNSQRSVGYGDGVYRSPDGGKTWENVGLKASEHVG
                     KILVHPKDGKTVYVAAQGPLWADGGDRGLYKTTDGGKTWKAVLTISPKTGVSDVWMDP
                     RDPEVLYATAYQRRRHVFTLINGGPESGIHKSTDGGATWTKLTNGLPKGDVGRIGLAI
                     PPTAPDTVYALVEARGKDGGFYRSLDGGMSWEKRGEYATGSAQYYQELFPDPHDASRV
                     YSMDVYMQVTEDGGKTWRDAGEKAKHVDNHALWIDPEDPNHLLNGNDGGIYETWDRCE
                     TWQFKTNLPVTQFYRVAVDDARPFYNVYGGTQDNNTLGGPSATTKVHGIGNEDWFVTQ
                     GGDGFFAAIEPGNPDIVYSEAQHGVLVRYDRKTGEQVDVQPQAGPGEAPLRWNWDSPL
                     FISPHKPTRLWFAAQRVFRSEDRGNSWKAVSPDLTRQVDRNRLPVMGRVWSVDAVAKN
                     ASTSFWGNIVSLAESPKKEDLVYAGTDDGLVQVTEDGGGTWRKVATFPGVPEMTYVAD
                     LAPSPHEADVVYAAFDNHKRGDFKPYVLKSADRGKTWVSVAGDLPARGTVYTVAEDPG
                     RPGLLFCGTEFGLFFSHDGGKRWVQLKGGMPVINVRDLAIQEREGALVAATFGRGFRV
                     LDDLTPLRLATPETLAKEAVLFPVRDAWAYIPRVPYGLKGKAFLGESYYAAENPPFGA
                     VFTTYLKEELKGRKKARQDAEKEKAKKGEDVFYPTWEELRAEDREEEPTLLLTVTDEA
                     GNVVRRLTAPAKAGFARIAWDLRYPASNPARLKPAEESPWYPGPRGPMVAPGTYRVAL
                     AKKVDGVVTPLGEPQSFRVVPLGAATLGEVDRAAVLAFSKNTARVQRAALGAVEAAKE
                     AKGRVDHLEKAFLDTPAAPAELFGEVRRLKLALADLDVVLNGDSTLGRRNEPALPGLV
                     DRVQTVVYSHWSTTAAPTATQEKEVETAAAGIGTALETLRSIAKEISALEAKAEALGA
                     PWTPGRIPELK"
     gene            complement(16306..18447)
                     /locus_tag="PLP50_10235"
     CDS             complement(16306..18447)
                     /locus_tag="PLP50_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA51966.1"
                     /translation="MKQIPTDLVPALLLVCAFAAAFVPREAEAQGRIVYPPTRTVDAK
                     DVLHGVTVPDPYRWLEDVKDPEVQAWMKAQDDLTRKELAKAPERARLLARLKELAYLD
                     SVSAPAKRGNHFFLTRRHATKEKSVVYWREGKDGEEKVLFDPNGWSKDGSVGLGDWTP
                     SWDGTKVAYQLKENNSDEATLHVIDVATGKVSEVDVIPGGKYATASWTPDGSGFYYTW
                     LPSDPKIAVDERPGHQEVRFHRLGEDPTKDRVVHGKLGDPTAFLSAALSRNGRWLVLY
                     VQYGWTSNDVLFRDLADPAQADRWTPLVVGTEAQWGVEVLGDRFFVTTNDGAPRSRVF
                     EVDPLRPARKEWKEVVPERKDATLVSASVLGGRLALNYLKDASSLLEVRELDGALVRE
                     VKLPGLGAVGGPAGLPEEDEAYYSFESYTTPREIHAMSMKTGAQELWFRLKVPVDPSA
                     YEVEQVFFPSKDGTRLSMFLVHRKGMKRDGKARTILYGYGGFQVSETPRFSAMLYPWL
                     EEGGVWAAVSLRGGGEYGEEWHRDGMLLKKQNTFDDFLAAARWLSAEKVTSPERLAIY
                     GGSNGGLLVGAAMTQEPKAFSAVLCAVPLLDMVRYHLFGSGRTWISEYGSADDPKQLA
                     ALLAYSPYHAVKKGTAYPATLLLSADSDDRVDPMHARKLAAALQAATTGGPVLLRIER
                     NAGHGGADLVKSAVESRADEFAFVLAHTKGK"
     gene            18540..19847
                     /locus_tag="PLP50_10240"
     CDS             18540..19847
                     /locus_tag="PLP50_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51967.1"
                     /translation="MTRLSALLAVAALAGGPLAGAGARPAPPEVVAAPDGLAFALALG
                     GGGQVLASGPYLLRVPGAEGPLRLEMARLAVDGARLRGELRLSNDSGSLLSGLELDFR
                     SVTPTVRAVPGRAAPEPLPLALPRPLSVGELLPGEATPFVPFEIAPLPLGEDVTVVVL
                     LGAVTGLAVSPPIAVEGALRPTALDTDRSGRLYVATAGVGRVLRLTAASAATPAEAAR
                     PSAPPTGMALRRRDHGLFVATGGATVEVYRPGRTRPATLDAGRPVTLLRVDASGVLRA
                     ASGNGVLAFDEAKAGPPRPLGPEGSEVLSFDADARGRVRAVVRNGETRRVVASEAAGA
                     SPLAAGRGPGADALDAPRAVRFAADGALWIAADGPTPESAVLTRFSPNGEPRGTLPRL
                     GLALLLGKDEEAGVPPAVDLAPGPDGLLWVLLESGALFGARPF"
     gene            complement(19873..20295)
                     /locus_tag="PLP50_10245"
     CDS             complement(19873..20295)
                     /locus_tag="PLP50_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51968.1"
                     /translation="MEITRKSFLSGAAAGLAGLTLLPAEALAAPRAKVEEGLLGLRSA
                     AGETFTARSAEGATVTLLLERVEELKSGTDVEQFRLLFSADGPYRLAEGTWRLVGTRG
                     RRVGDLFLVPAGTNQAGQALYRADFCLLSKLVPAAPRP"
     gene            20409..20717
                     /locus_tag="PLP50_10250"
     CDS             20409..20717
                     /locus_tag="PLP50_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51969.1"
                     /translation="MTDTLSISDFAPLVGAAFTIRFPDGTLELTLGTVEPLGSRAPRA
                     DVPALRTEPFSLVFLGPLAPVLPQRTWALEHPVLGTHEVFLVPLGPQEGRMRYEAVFN
                     "
     gene            complement(20710..>21286)
                     /locus_tag="PLP50_10255"
     CDS             complement(20710..>21286)
                     /locus_tag="PLP50_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA51970.1"
                     /translation="SPCRASSRHGPEGRVTGAKAAVTGVAAEESAEARRVALRPAEPA
                     DRELLFRVYASTREEELAPVPWPPETKEAFLRQQFDAQDAWWRANYDGASFEVVVADG
                     RDVGRLYLWEGPSEVRIVDVALLPQARRGGIGTSLVRDVQARASAAGKSVTIHVERMN
                     PALRLYERLGFRLVEDKGVYLFLSWSGDAVS"
BASE COUNT         2802 a         7928 c         7746 g         2810 t
ORIGIN      
        1 tggaggggcg cacggcgcag ctgaagatcg acaaggccga gcgcgagctc gccgccctcg
       61 agctgaaggc gccgcacgac gggatcgtcg tcctgaagcg aaactggcgc ggcgagctgc
      121 cccaggtcgg gcaggtcgtc tggagcaccc agacgctcgc ggagatcccc gacctcggca
      181 agctcgaggc cgaggtctgg gtcctcgagg ccgacgccgg agggctcgcg cccgggcgcc
      241 ccgccacgct ctcgctcgag ggcgaggccg gccggcccgt ccccgcgaag gtgaagtcgg
      301 tcgacgctct cgcccggccg cgcctccgga acgtcccgat ccagtacttc ggcgccgtcc
      361 tctcgctcga cgcgatcgac ccggcccgga tgaagccggg ccagcgtgtg gccgcgacgc
      421 tcgacctcgg cggcgaggcc gacgccctcg tcgtcccgag gcaggcgctc ttcgagcgcg
      481 gcggcgaaac cgtcgccttc cggctcggga agggggcctt cgagcccgtt cccgtcacgc
      541 tcggcgcgac cgcgctcggc cgcgtcgccg tcgcctcggg cctgaaggcg ggcgacgtcg
      601 tcgcgctcgt cgacccggag gcggcgcccg agccggccac gtcgcccgcg cccggctccc
      661 ccgtcggagg ggcgtcgtga gccttcgcga gtcgctcctg cgcgccctgg cgagcctctc
      721 ggcgcacaag ctgcggtccg tcctgacgat gctcgggatg atcttcggcg tcggcgccgt
      781 catcgcgatg ctctcgatcg gcgcgggcgc cgagcggcag gcgctcgaca tgatccggcg
      841 gatggggatc tcgaacgtcc tcgtccgcgc gaaggagttc cgccccgacg agctccagga
      901 ggtccggaag aagtcgatcg gcgtctcgct ccgcgacgcc gccgggatcg aggaggccgt
      961 gccgggggtc gacttcaccg gcccgcgcgt cacggtgaac ccctggagcg tgcgggggcg
     1021 gggcggaaag agcgaggcgc gcgtctcggg cgtctcgtgg cgccacgcgg agctcgtgtc
     1081 gctgccgctc gccgccgggc gtttcctgac gaaggaggac gaggagacgc acgcccaggt
     1141 ctgcgtcctc ggcgacgacg cccgccgcga cctcttcggc tgggagccgg cgctcgggcg
     1201 aacggtcaag gtgaacgacg tctggctcac cgtcgtcggc gtcctcgctc cctcgggcga
     1261 gggcggcgac tcgtttcagg gggtctccgt cgggtcgacc gcgcgggagc tcttcgtccc
     1321 cttcacgacg gtcctccgca agctggaccg cgacgcgctc gagtccccgc tctccgagct
     1381 cgtcgtgaag ctccgcgacg ggaccccggc gacggaggcc gcgcgcctcg tgggagccct
     1441 cctcgaccgg ctccacggcg gcgcggccga cttcgaggtc gtcgtccccg aggcgctcct
     1501 cgcccagagc cggaagaccc agcgtctctt caacgtcgtc atggggtgca tcgccgggat
     1561 ctcgctcctc gtcggcggga tcgggatcat gaacatcatg ctggcgaccg tcctcgagcg
     1621 gacgcgcgag atcggcatcc gccgcgcgat cggcgcgcgt cgaaaggacg tcctcgtcca
     1681 gttcgtcgtc gaagcgttcg cgctgagcgc gctcggaggc ctcctcgggg tcggcctcgg
     1741 ggtcctcatc gcgaaggtcg tcgccgcctc cgccggctgg ccgaccgtcg tgacgctctg
     1801 gtccctcctc ctgtcgaccg gcgtctccgg cgccgtcggc ctcctctccg gcttctaccc
     1861 ggcgctgcgc gccgcctcga tcgacccgat cgaggcgctc cgctacgagt aggcgcgcgt
     1921 cagcgcgcga ggagcccgag gagcgcggcg tagtcgcccg gaagcggcgc ctcgacgtcg
     1981 atcccgagcg cgggaacgac gacccgggcg gcgtggagga gcgggtgcgc cacggccgcg
     2041 aaggcggcgc ggagcgccgg gtcgcggacg ccgcgccagc gcgtgccgcc gccatagagg
     2101 tcgtcgccga cgagcgggtg tccccgcgcc gcgaggtgga cgcggatctg gtgcgtccgg
     2161 cccgtctcgg gcgcgacctc gaggtccgcg acggaagcgt accggcgaag cgtcgtccat
     2221 cgggtgacgg ccggcttgcc ttcctcccgg acgcgcatct tccggccgtc cttccggtcg
     2281 cgtccgagcg gagcgcgggt ctcccctcgc accggcacgg gatgccccca gacgagcgcg
     2341 cggtacgtct tctccgtcgc acgcttctga aacgcctccg agagacgccg gtgcgtcgcg
     2401 gcgtcacggg ccaggaggac gactcccgag gtcccctcgt cgagccggtg gacgaggagc
     2461 ggtcgcgggg ccgtctccgg caggccgcag gaggcgagca gccgcgagac gacctcctcc
     2521 tccgacttcc ccggtcggga agaggtcgcc atcgagacgc cggcgggctt gtcgagggcg
     2581 aggaccgagc cgtcgtcgaa gaggacgcgg tccgggaccc gcggcgccgg cgcggcgtcc
     2641 gccccggcgc tcccggtcac ggcggcgtcc ccgggacgcg gcggatcgac gcgtcgtcgc
     2701 tcgggacgcg ctctgcggag gtcgccccgg gcggagcgcc gtgcgcgacg aggactcggt
     2761 tccggcccgc ggccttggcg gcgtagagcg cggtgtccgc ctggcgcagc caggccgaga
     2821 cggacacgct cgccctcacg ttcgggtcga agacggcgac gcccgcggag acggtcgtcc
     2881 cgtcacgcgc cccggaaacc cgcgcccggg cgacgccgtc ccggatcctc tcgagacagg
     2941 agacggcccg ggtcgcgtcg gcctccggca ggaggacgac gaactcgtcg cccccgaagc
     3001 gggccgcgag gtcggtgtcc cggatcgcct gcgtgatcgc ggcggcgacc gcgacgagga
     3061 gccggtctcc ggtcaggtgg ccgtgccggt cgttgaaccc cttgaacccg tcgaggtcga
     3121 ggatcgcgag cgcgacgggg tgggcgctcc tccgggcccg ggcgagctcg aggccgatcc
     3181 ggtcgaagag gaccgcccgg ttcgccaggg aggtgagcgg gtcggtcgag gccgcgagcc
     3241 ggagacgcgc cagccggtcg gcgagcgccg cgaggaggac gctcatcagg aggaagaagg
     3301 ccggcccgga gagggcgatc tcgatcgacg ccccgagtcc gagggcgagg agaccggcga
     3361 cgtcgaacgc cgccgccagg accgcgacga gcgccgcggc gaggagggac cgcgcgtgcc
     3421 ggacgcgccg acgcgccgcc gtcccgaggt gcgcgaggac gtcggccgac gcggccgcgc
     3481 cgaggaggcc gccgagaggg aacgtgtagt agatgtcgtc gggcctcggc cagaggaggc
     3541 acgccagcgc cccgaccacc tgcgccgaca gcaggacgcg gtggaggctc ccgatcggac
     3601 gctcgaagaa cttcaggaag aggacccagg cgagcgccgg caggacgaag acgccggcat
     3661 gcccgacgcg aaagaggaac gtcaggggca ggccgaagac ggcccagacc gagagccagg
     3721 agacgatgag gacggccgtg acgctcgtcg tcaggaagaa aaagaggaag tccgactgcc
     3781 cccggtcgcg caggtagaag aagagggaga agagcccgag cgcgccgaat gccgcggcga
     3841 ggagggcgcg gggcgcctcg cgcatcgtcc gggcgtggaa ggccgcggag accgcctcga
     3901 gccgcggagt gccggtgatc ccgcctccgc gcggccccgc gttgtagacc cgcacggcca
     3961 ggacgtgacg tcccgggacc gaggaccgcg acgggggaag ctcgtagatc cgctccacga
     4021 ccgtcccctt gtcgtaagcc ggcgggaacc cgccggtccg gccgatcggc tggccgtcga
     4081 ggtagacctc gtccgcgtcc cggatctgcg tgcaggtgaa cgcgagcggg acgatcgaga
     4141 gcgagggatc gaggtcgagc gtgacccggt accagcccgt cccgtgtccg gagaagccgg
     4201 cggactgcca gctcccgggc acggcgatcg ggcgaaacgg gagagtgtcc aggtccgcga
     4261 gcccgccggg cgggtccccc agcgcgaact cccaggtgcc ggcgaggctt gccggcaggc
     4321 gctcgacccg tatcgccgcg ggctcctgcc cttcggccgt gcgcggcacg ggaatggccc
     4381 agaggagcgc cgccgccaga acgcacgttc gccggaccat ccggatcagc atctcccatc
     4441 cgcgacgaag gggcaacggc cgcccaggag gcgggtcgac ggggcggata agatcggccc
     4501 cggaggcccg ccatggagct cagggattcg gtcgccctcg tcaccggcgc cggccgcggc
     4561 atcggccggg ccgtcgccct cgccttcgcg cgcgaagggg cccgagtcgc cctgatgggc
     4621 ctgaccgcgt cgcacctcgc gtccgtcgag aaggaggcgg gcgctctcgg cgcgcggtgc
     4681 gccgtcctcc ccggcgacgt ctccgacgag gggagcgtcg cgcgctgcgt ggccgcggcc
     4741 gagcagcagc tcgggccggt cgacgtcctc gtcaacaacg cgggcgtctg ggcgcacggc
     4801 ccggtcgacc ggctcgacgc cctcgtcttc gaccacacgc tcgcggtcaa ccttcgcgga
     4861 ccgttcctga tggcgcgcgc cgtcctcccg gggatgaaga gccggcgccg cggccacgtc
     4921 gtcaacgtcg cgtcgacggc ggctctccgc ggcttcgccg gcggcgcggc ctactgcgcc
     4981 tcgaagttcg gcctcgcggg cctctcgcag gcgatgctcc aggacgtccg ggggagcgac
     5041 gtgcgcgtca cctgcctcat gccttcgacc gtcgcgaccg acatggccct ccggggcgac
     5101 ttcctgaaga gcccggagac ggcgatccgc cccgaggacg tcgcgcaggc ggtcgtcgac
     5161 ctcgtccggc tcgactcccg cgtcctcccg gtctccctgg agctctggca gacgaacccc
     5221 tgaacgcgcg gggccacgcc cgggctcagg gggcgacgac cctgtagacc caccccatgt
     5281 cgcgcgtccc cttcgccgcg agctccgcgc ggacgccttt ggcttcttcc gccgacggga
     5341 actccccgac catgacgcgg aaccagatcc ccttctcgcc gaggtcgacc tccgcgacgt
     5401 agccctcgcg cccgacgagc gcctcgatcc gcttcaggtc ccgctcggcg gcgccacgat
     5461 cccggtacga ggtgaagtgg atcgagaaga cctcggggcg accggcccgg tcgcgagtga
     5521 cgacgagacc atcccggctc tgccggaccg gtgcgggcgg ggccgtcttc acggggacgg
     5581 gcatcggcgc caccagcgcg ggcgcgaccg tcgggacggc gcccgggcgt tctctgggag
     5641 cggtcggcac ggggacggcg acggaagccg gggccgtcgc gccctccgcc cccggcgcgg
     5701 ctggcaccgc gggctccggc gtcggcgact ccacggggag cgcctccctc tcggaggcct
     5761 cccgggccgg cgcctcggtc ctctccgggg cctgccgggc cacggcgacc cgaccggagt
     5821 ccgcgccccg gtcgatccac agcccgacga ggaggtagcc gagcgccgcg agagccgcga
     5881 gagccgcggc gatcccggcg agacgtcccc atcgcggccc gccctcgcgt gtcgcaccgc
     5941 gacgtcctcc gcgaggaatc gtcaggacgg tgtcttcgtc gtcctcgaat ggtggaggca
     6001 ccggctcgtc cgccccgggc ggcgcgtcga gaacggcggc cgtcgccgca gccccgaact
     6061 cctcctcccg ctccgggggg ccggcggccg cgcccccgcc cgcgaggacc gagcgcaggt
     6121 cgatcgactc ggcttcccgc gagaggcgcg tcagggtctc gtctccgatc tcctcctgcg
     6181 gctcggccgg gaagtccgga ggagccggcg ccgcctcgtc ctccggcagg gcgaagacga
     6241 aaccgccgcc ctcgggcgcg cggcccgcgg gctcctcggg gaccgtgaac ggcccgcccg
     6301 ccacggaatc gaccggctct gcctcgggag cggagacggg gacctcgtcc gccgggagag
     6361 gaggaggagg ctccgcctcg agcggaaccg cgacggccgg agcggcgagc ggttcgcgcg
     6421 cctcgcggtc ccagcggagg tccagcgcgc ccgtcgtctc ggagaacggg tcgtccccgg
     6481 agggcggaga aggcggctcc ggctcgaagc tcgcggccgg aacgagcggc ggcggcgccg
     6541 gcacgacgag cggcggcggc acccccgcca cgcgcgcgag cgcccgctcc acgaggacct
     6601 cgatcggaac gctctccccg ccggcgtcgc ggatcctctt cagctcctcg gcgcagacgt
     6661 cccgcgccgc ccggaaagcg acgtcgggct cgaccggcgc gcgcgccgtg agctcgcgga
     6721 ggacctccga gaccgacggg agcatgcgcc tcacgcggga ggcggtgagg tcttctccgc
     6781 tcatccggcg agtcgctccc agtccccttc cggagcgtcg gcggcgatcg cgagggagcc
     6841 gctcgcgccg gcgaacaccg ggtcctccac cgaccggacc cggccgccgc cgacctcctc
     6901 gagcgcccgc tcgagggccg ccgcgaggcc ggggatctgc gagccgcctc ccgcgaggac
     6961 gacgctattt cgcacccgct gctggtactc ggggtcgacg cgagagagga gatcgagcat
     7021 cgtctcggcg aggggcccga cgaggctctc gcacgccttc ctcatctccg ccgtcacctc
     7081 gaggcgggtc ggcttcccgc ggaccggggc ctcgacgaag accggctcct tcggctcgcc
     7141 gacgaagctg ttcctctcct tccactcccg gaccatgtgg atcgagaagt tcgcgtcggg
     7201 gtggcgcgcg cggatctgaa gccccagctg ctcgtcgacc gtgtcgcccg cgtgggtgag
     7261 cgtccgctgg tcgtcgtcgg tcgggtagcg gccgttcatg acgcagaagt cgctcgtccc
     7321 ggcgccgatg tcgatgatca tcgtgtggag gagcgcgtcc atgccgtagg cgacggcgaa
     7381 cggctccgac accagcagga gcgcgtcggc gacccccttc acggcgttcc gcaggtgctg
     7441 gcgggagacg cgcatcgcct cggccgggac cccgacgacg gcctggacgg tcttcccggc
     7501 gccgccggcc ttctcgatca ggtgcgcgag aagctccctc acggccgcct cggccttcgg
     7561 ggagccctcc ttgatgaggc ccctctcgag cgggcggtgg aggtcgagca tcggccggtt
     7621 ctcgagcgcc tcgtgcccga agacgaccgg cttcttcacg accttccggg cgaccatgtc
     7681 gacgggccat ccgacccagc tctccacgac gtgccgctcg ccgttcgagg cacagatcgc
     7741 gctgcgcgag gtgccgaggt cgatcccgac gtggaggacg tccttcttgc cgctcatccg
     7801 tttccgctcc ttcccgctcc cgcgaggttg cgtgcgtcgg cgtaggcgcg cacgccccgc
     7861 gcgagcgcct cggcgatcgc ctcccggtac gcctcgtcgc ggaggaggcg cgcgtcctcc
     7921 gcgtgcgaga cgctcgagac ctccgccagg atccccggca tctcggaggc gatcaggacg
     7981 acgaacgggg cgctcttcac gccgcggtcg ccggcctccg gcgtgacctt ccgcgtcgag
     8041 gcgacgagct cggcctggac cgactcggcg agccgtctcg actccgcctg gcgaacgccc
     8101 acgtagacct cctcgagaag ccccctgaag tccgaaagag aatagcccga ggaaaggttc
     8161 tcggcgctcg cgagccggcg gatcgcgggg tcgtccgtcg ggccgaggta gtacgtctcg
     8221 acgccgcggc gatccgggtt ggggatcgag ttgacgtgga tcgagacgag aacgtctccg
     8281 cccgcgtccc gcgcccttcg cgatcgctcg cgcagggaga ccgtctcgtc cccctcacgg
     8341 gtcatcacga cgcgaaagag gcccccgcgg agcttctccc tcacccgccg ggcgacgtcg
     8401 agcgtgacgt ccttctcggg gaggccgtcg gcggtgagcg cccccgggtc gacgccgccg
     8461 tgtccggcgt cgaggacgat cgtcctcacg tcgaggtgga agagcgtcgg gtcgatcgac
     8521 ttcggcgcgg gccagtcgag cgtctcgagc cccggctcgg gagatggggc gaccgtccgc
     8581 tgcggcacga cgacgacctc cgtcggggtg acgcccccgc cggagaaggc gttgatcgac
     8641 gcgaagagcg cgagcgggat cgcgaccagg gccgcgccgc gcaccgccct gccgacccaa
     8701 cgccctcccg ggcgggcgcg cgccgggggc cgtccctcga tgacgtcgag gttctcccgc
     8761 acggcgtcgc ggagcatctg cttcttgagc cgctcggcgt ctcgcatggc caagctctca
     8821 ttttcgcagc ttttcccgcc cgcggcgggc cgccacggcc cgtcagggcg cgacgaggac
     8881 cgcccgaacc ggggagccgt ctcctccctc gatccggagc ggcagcgccg agagctcgta
     8941 gcggcccggc gggacgtccg cgaggacgag cccctcgagc cagcagacgc ccgcggcgac
     9001 gagcgcccgg tgcgccgtgt aggcatggtc gtcgaacggg tcgacggagg gggtgtcgat
     9061 cccgacgagg acgacgccgc gcgccgcgag cgccggggcg agctccggat cgagccccgc
     9121 gaaggcgtcg tcgaaccgct cccggctccc gaacgtcccg gtcttcagga ggacccgggg
     9181 cgcggacggc ggttcggcca ggtcctcgag gcgcacgcgg ccccgcggcg acacccgcgc
     9241 ctccacgacc tggcaggtcc cgatccacgt ctcgagggga acctcgtcga tcgcggccgc
     9301 cccggccacc atgtggagcg gcgcgtcggc gtgagagccg aggtggagcg tcgtccggat
     9361 cgacgagaag gtcgtcccgt cgggaggcgt cgtcacctcc cgcgagaacg ggacgtcgcc
     9421 cggccagacg gcgatccgcg gcgacaggac cggagagagg tcgtagatcc gcgccacccg
     9481 tccctcactg cctctcgagc cacgccgaga cgtcctcgac catccggtcg aacgaggggg
     9541 agacgaagag gagctcctgg taccgcgtcg ggtcgtacgg gatcgcggcc gccgcgtcgc
     9601 agtcgagcgg gcggatctcg gcgttcgact cgaacgagcc gagctcgccg aacgaagaga
     9661 ggagcccggc gccgaccgcc ttcctctgcc cctcctcctc gacgaggccg aactcgagcg
     9721 tgtaccagta gacgtattcg agccgcgcga gcgccgcgtc gtcgacgcgc cacgccgcct
     9781 cgccgaaacg ccgggagagg gccacgatgc cggggtgaaa gaggctcgcc gcgtggccga
     9841 cgagctcgtg gacgacgtcc ggctcgggcg tgtagagcgg cacggagtgg tgacgcatgt
     9901 actgcgtcga gaggaagacc cccttcccca tcatcccgag gaaggtccgc ccctggacga
     9961 ggccggccac gggaagcatc tggaagccgg cgcctctctt cagcgcctcg ttcacctcgg
    10021 aaagctgcgg cacgcaccct cggtcgagcg cgagggccct cgagctctcc ttccattccc
    10081 ggcacgcgaa cctctcgtgg agcggcgcga ggtgctccca gacggtcctc cagaccgcct
    10141 gctcgtcctc cgtgtactcc acgtcgggca gcggctggcc gtcgcggtac tcgagcgcga
    10201 gccgcgcgat cgcgttcctc cgcgcccggt agaccgggtc cttgaagccc gggtggtccg
    10261 ggtcgagcgg gacgagctcc tccgaccctc cggcgtgagg ggaggcgccc cgcgccgcct
    10321 cgtcgatctt ccgcgtggcc tcgctcatcc gatccgtccg tccggcgggc agtctatccg
    10381 agaggacccg ggtttcgccc cggcggtcgg gcgcctctca cgcggcgacg tctccgccca
    10441 gaaggctcgg tcctctaccg cggatgcggc tgcgtccgcg cctccacctc gcccaggagc
    10501 tctctgagcc ccggccgggc cggcgcgagc gcgagcgcct cgcgcaggaa cggagcggcc
    10561 gcggccggat cgccgcgcag aacgtacgcc gcgccgatcc gatggagaag cgcagggacg
    10621 atcggatgca tcgaagggag gccgaagagc gcacggagga acaggggaga gatgaactgg
    10681 ccggcgcctg gagcggggac tcgctccgtc gccccggcgc gtgagaccgg agacgccagg
    10741 gagagcccga gcgagcgccc gagcaggaac gcgtgccccg agatcggcga cagcgagggc
    10801 acgaccgaca cgatctcggg tcccgaccgg ggccagctcg acccctccgg aggccggctc
    10861 agctccaccc acgacatcca cgctccggcc gccacgaaga caccgagcgc gttcactccg
    10921 accccggccg ccacggccgg caggacggct cgtcgccgcg cgagaagcgc cggcgccgcc
    10981 aggagcggca gcaccggaag cgtgtgacgg gggccccacg ccgagccccc gtgccagccc
    11041 tcgaaccggg cgacggtcag gaccaggagg agcggcgcga cgccgcagag caccctcacg
    11101 gccagcggaa ccccgcgaaa gagaaggcga gcggcgccga cgacggcgaa gggcgcgaag
    11161 aacagcagtc cccggcccgg ggagagaagg agcccgtaga ggccctggag aagcggcgtc
    11221 gtgaatctca gcgcctcgtc cccgtagccg aagttctcgc ctccaccgaa ccggagagcg
    11281 ctcgcggtcc agctgacggc gagcccgcat gcgacgagcg cgccgaagac gaaggttcgc
    11341 cgaaggccgc cggcgcgccc gcgccacgcg agggccgctc cgaggacgaa gaccggcacg
    11401 aggatccaga ggacgggctt caggagcgca gctccggaga acgccgcggc ggcgaggaac
    11461 gccgggcgga gtccggttcg acccccaccc tcgaggatct ccgctgcggc gcacgcgagc
    11521 cccgcggcag cgaacgtctc gaagaaggag tccgccgcat acgcccagag gaaggtcgac
    11581 gcgagaaacg cgggacccca ccacggggag gattccggga ccaggaaacg ggcgaggcgc
    11641 cggaacgccg cgaccgccag cagcgtcccc gtcgtccacg cgagcgcgat tccggcatcg
    11701 agcccctgcg ctccgaggac gctccgaagc gcccaaggga ccgcgacgaa cgggagaagc
    11761 aggagcgtcg ggaagagacc gtagtgcgag cggaaggcga aggcgctgtc cgggccgagc
    11821 tccgcagagg ggagcttcgc tgcctcgaat cgcccctgga ccaggaaccc gagcgtgtcg
    11881 gagatcgcct ccgcggcgtc ccacgagacg acggagcgac cgatgaaggc cgcgccgagg
    11941 agaaggacgg tcgcgatcgc cgcggcgcgg acagaggccc ttccgccgcg cacgggtacc
    12001 ctcagcggac ccgcttccgg cgcggcagga ggtcgttcag ctcccgcagg gccatgtcga
    12061 gccccttctc gacgcccggg tcggtctcgc cccggagcgc ctgcatcgcc gcctcgtcgc
    12121 gaagccgctg caggcggtgc gccagggccc ggaggatcgt ctcgacggcc ttctgctcgt
    12181 cgtcgcttcg ctgtttcgcc acgcgggcat tctggacgag accggcgccg ctttggaaga
    12241 tgatccggcg gtgagcgtcg acgtcctcct cgtccgcacc cacagccccg ggaacctcgg
    12301 ctcggccgcg cgggcctgca aggcgttcgg cgcgggcctc ctcctcctcg acccgcgcgc
    12361 tgacgccgct cacgaggacg cccgggcctt cgcgtcgggc gcggaggacc tcctcgacgc
    12421 cgcgccccgc ctggcctcgt gggaagaagc cgaagacggg gccggcgcca tcctcgcgct
    12481 cacctcgctc cgcgggcggg cgacacgcgg gcttccgccc gcctggagct gggccgcggc
    12541 ccgacggaca gccgggagcg ggaggctcct cctcgtcttc ggcccggagc gctccggtct
    12601 gacgacggag gaggtgagga ggtcgcaggg gcggctcgcg cttccgacgc ggcccggctt
    12661 cccggtcctg aacctcgcgc aggccgtggc agcggcgctc gcgctcctcg agggcggacg
    12721 gtccgcgcgg ggcggggcgg aggccggaga gccggcgggt cgggacgcgg cctccgcgga
    12781 ggcgcaccgg cgcctccggg aggagctcgc caccgctctc cgcgcctcgg ggttcctggg
    12841 ccgcgaggac gacgccgtcc tcttcgagct cctcgcgccg ctcctgcgcg cccggccgac
    12901 cgggcgggag gcggagctct ggacgggcgc gctccggaag ctcgggcgac gcgtgcgggg
    12961 cgcgtagagg aagccctcca cgcgccccgc cgctcaacgc ttcacttcag ctcggggatc
    13021 cgtccgggcg tccacggggc gccgagcgcc tccgccttcg cctcgagggc cgagatctcc
    13081 ttcgcgatcg accggagcgt ctcgagggcc gtgccgatcc cggcggcggc cgtctcgacc
    13141 tccttctcct gggtcgccgt cggcgccgcc gtcgtcgacc agtgcgagta gacgaccgtc
    13201 tggacccggt cgacgaggcc cgggagcgcc ggctcgttcc gccggccgag ggtcgagtcg
    13261 ccgttcagga cgacgtcgag gtcggcgagc gcgagcttca ggcgccgcac ctcgccgaag
    13321 agctcggcgg gggcggccgg ggtgtcgagg aacgccttct cgaggtggtc gacgcgcccc
    13381 ttcgcctcct tcgcggcctc gacggccccg agcgcggcgc gctggacgcg ggcggtgttc
    13441 ttcgagaacg cgaggaccgc ggcccggtcg acctcaccga gcgtcgccgc gccgagcggg
    13501 acgacccgga acgactgcgg ctcgccgagc ggcgtcacga cgccgtcgac cttcttcgcg
    13561 agggcgacgc ggtaggtgcc gggggcgacc atcggcccgc gcggcccggg gtaccacggg
    13621 ctctcctccg cgggcttcag gcgcgccggg ttcgacgccg ggtagcggag gtcccacgcg
    13681 atccgcgcaa agcccgcctt cgcgggggcg gtcagccgtc ggaccacgtt ccccgcctcg
    13741 tcggtcaccg tcaggaggag cgtcggctcc tcctcgcgat cctccgcgcg gagctcctcc
    13801 cacgtcgggt agaagacgtc ctcccccttc ttcgccttct ccttctccgc gtcctggcgg
    13861 gccttcttcc tccccttcag ctcctccttc aggtacgtcg tgaagacggc gccgaacggc
    13921 gggttctcgg ccgcgtagta cgactcgccg aggaacgcct tccccttcag gccgtacggc
    13981 acccgcggga tgtaggccca tgcgtcgcgg accgggaaga ggaccgcctc cttcgcgagc
    14041 gtctcggggg tggcgaggcg gagcggcgtc aggtcgtcga ggacgcggaa gccgcggccg
    14101 aacgtcgccg cgacgagcgc cccctcgcgc tcctggatcg cgaggtcgcg gacgttgatg
    14161 accggcatcc cgcccttcag ctgcacccag cgcttcccgc cgtcgtgcga gaagaagagg
    14221 ccgaactcgg tgccgcagaa gagaaggccg ggccggcccg ggtcctcggc gaccgtgtag
    14281 acggtgccgc gcgccgggag gtcgcccgcc acggagaccc acgtcttccc tcggtcggcg
    14341 ctcttcagga cgtacggctt gaagtcgccc cgcttgtggt tgtcgaacgc ggcgtagacg
    14401 acgtccgcct cgtgcggcga cggcgccagg tcggcgacgt acgtcatctc cggcacgccg
    14461 gggaacgtcg caaccttccg ccacgtcccg ccgccgtcct ccgtcacctg gacgaggccg
    14521 tcgtcggtcc cggcgtagac gaggtcttcc ttcttcgggc tctcggcgag ggagacgatg
    14581 ttcccccaga aggacgtcga ggcgttcttc gccacggcgt cgacgctcca gacgcgtccc
    14641 atcacgggga gcctgttccg gtcgacctga cgcgtcaggt cggggctgac ggccttccag
    14701 ctgttcccgc ggtcctcgct ccggaagacc cgctgcgccg cgaaccagag gcgcgtcggc
    14761 ttgtggggcg agatgaagag aggcgagtcc cagttccagc gcagcggcgc ctcgccgggg
    14821 ccggcctgcg gctggacgtc gacctgctcc cccgtcttcc ggtcgtagcg gacgaggacg
    14881 ccgtgctgcg cctcggagta gacgatgtcg gggttccccg gctcgatggc ggcgaagaag
    14941 ccgtcgcccc cctgcgtgac gaaccagtcc tcgttcccga tgccgtgcac cttcgtcgtg
    15001 gccgacggcc cgccgagcgt gttgttgtcc tgcgtgccgc cgtagacgtt gtagaagggg
    15061 cgcgcgtcgt cgacggcgac gcggtagaac tgcgtcaccg ggaggttcgt cttgaactgc
    15121 cacgtctcgc agcggtccca cgtctcgtag atgccgccgt cgttgccgtt caggaggtgg
    15181 ttcgggtcct ccgggtcgat ccagagggcg tggttgtcga cgtgcttcgc cttctcgccg
    15241 gcgtcccgcc acgtcttgcc gccgtcctcc gtcacctgca tgtagacgtc catcgagtag
    15301 acgcggctcg cgtcgtgcgg gtcggggaag agctcctggt agtactgcgc cgagccggtc
    15361 gcgtactcgc cccgcttctc ccagctcatc ccgccgtcga gcgagcggta gaagccgccg
    15421 tccttcccgc gcgcctcgac gagcgcgtag acggtgtcgg gcgcggtcgg cgggatcgcc
    15481 aggccgatcc gcccgacgtc ccccttcggg aggccgttcg tcagcttcgt ccaggtcgcg
    15541 ccgccgtccg tcgacttgtg gatgccgctc tccggcccgc cgttgatgag cgtgaagacg
    15601 tgccgccggc gctggtaggc cgtcgcgtag aggacctccg ggtcgcgcgg gtccatccag
    15661 acgtcgctga cgcccgtctt cggcgagatc gtgaggaccg ccttccacgt cttcccgccg
    15721 tcggtcgtct tgtagaggcc gcggtcgccg ccgtcggccc agagcggccc ctgcgccgcg
    15781 acgtagaccg tcttaccgtc cttcgggtgg acgaggatct tcccgacgtg ctcggaggcc
    15841 ttgaggccga cgttctccca cgtcttcccg ccgtcgggcg agcgatagac gccgtcgccg
    15901 tagccgacgc tccgctggct gttgttctcg cccgtcccca cccagacgac gagcgggttc
    15961 gtcgggtcga gggcgatgca gccgatcgag aaggagccct cggagtcgaa gaccggctcg
    16021 aacgtcgtcc cccagttcgt cgtcttccag acgccgcccg aggcgacgac gacgtagtac
    16081 gtccccttgc ggacggggtc gacggcgagg tcgccgatgc ggcccgacgt gagggccggg
    16141 ccgaggtcgc ggagcgcgag ccccgcgaac gtctcggcgg agtacggctc cttcggcttc
    16201 tcggccgcgg ccttcgagtc cttcgccgcg gccttcgcgg gcgcggcgag gccggccggg
    16261 gcgacgaggg agagggcgag gaggatcgga agagttctca tggcgtcact tccctttcgt
    16321 gtgcgcgagg acgaaggcga actcgtcggc ccgggactcg acggccgact tcaccaggtc
    16381 ggcgccgccg tggcccgcgt tccgttcgat ccggaggagg acgggcccgc ccgtcgtcgc
    16441 ggcctggagc gccgcggcga gctttcgggc gtgcatcggg tcgacccggt cgtcgctgtc
    16501 ggccgagagg aggagcgtcg ccgggtaggc ggtccccttc ttcacggcgt ggtagggcga
    16561 gtaggcgagg agcgcggcga gctgcttcgg gtcgtcggcc gagccgtact cgctgatcca
    16621 ggtgcggccc gagccgaaga ggtggtagcg gaccatgtcg aggagcggca cggcgcagag
    16681 gacggccgag aaggccttcg gctcctgcgt catcgcggcg ccgacgagga ggccgccgtt
    16741 cgagccgccg tagatcgcga gccgctcggg gctcgtcacc ttctccgcgg agagccaacg
    16801 cgccgcggcg aggaagtcgt cgaacgtgtt ctgcttcttc agcagcatcc cgtcccggtg
    16861 ccactcctcg ccgtactcgc cgccgccgcg gaggctcacc gccgcccaga cgcccccctc
    16921 ctcgagccag gggtagagca tcgcggagaa gcgcggcgtc tcgctcacct ggaagccgcc
    16981 gtagccgtag aggatcgtcc gggccttgcc gtcccgcttc atccccttcc ggtggacgag
    17041 gaacatcgag aggcgcgtgc cgtccttcga cgggaagaag acctgctcga cctcgtaggc
    17101 cgacgggtcg acggggacct tcaggcggaa ccagagctcc tgcgcccccg tcttcatcga
    17161 catcgcgtgg atctcgcgcg gcgtcgtgta cgactcgaag gagtagtacg cctcgtcctc
    17221 ctcgggaagg cccgcggggc cgccgacggc gccgaggccg gggagcttca cctcgcggac
    17281 gagcgccccg tcgagctcgc ggacctcgag gagcgaggag gcgtccttga ggtagttcag
    17341 cgcgaggcgc ccgccgagga ccgaggccga aacgagcgtc gcgtccttcc gctcggggac
    17401 gacctccttc cactccttcc gcgccggccg gagcgggtcg acctcgaaga cgcggctccg
    17461 cggcgcgccg tcgttcgtcg tcacgaagaa gcggtcgccg aggacctcga cgccccactg
    17521 cgcctccgtg ccgacgacga gcggcgtcca ccgatcggcc tgggccgggt cggcgaggtc
    17581 gcggaagagg acgtcgttcg aggtccagcc gtactggacg tagaggacga gccagcgccc
    17641 gttgcgcgag agcgcggccg acaggaaggc cgtcgggtca ccgagcttgc cgtggacgac
    17701 gcggtccttc gtcgggtcct cgcctagccg gtggaagcgg acctcctggt gcccgggccg
    17761 ctcgtcgacg gcgatcttcg ggtcgctcgg gagccaggtg tagtagaagc ccgagccgtc
    17821 cggcgtccac gacgcggtcg cgtacttccc gcccgggatg acgtcgacct ccgaaacctt
    17881 cccggtcgcc acgtcgatca cgtggagcgt cgcctcgtcg gagttgttct ccttcagctg
    17941 gtaggcgacc ttcgtcccgt cccacgaggg ggtccagtcg ccgaggccga cgctcccgtc
    18001 cttcgaccag ccgttcgggt cgaaaaggac cttctcctcg ccgtccttcc cctccctcca
    18061 gtacaccacc gacttctcct tcgtcgcgtg gcggcgggtg aggaagaagt ggttcccgcg
    18121 cttcgccggc gccgagaccg agtcgaggta cgcgagctcc ttcaggcgcg cgaggaggcg
    18181 cgcgcgctcc ggggccttcg cgagctcctt ccgcgtcagg tcgtcctgcg ccttcatcca
    18241 ggcctgcacc tcggggtcct tcacgtcctc gagccagcgg tacggatcgg gaaccgtcac
    18301 gccgtggagg acgtccttcg cgtcgacggt tcgcgtcggc gggtagacga tgcggccctg
    18361 ggcctcggcc tcgcgaggga cgaaggcggc cgcgaaggcg cagacgagga ggagggcggg
    18421 cacgaggtcc gtcgggatct gtttcatgcg gggacgctcc ttggatcggc acgcggtggt
    18481 catcgtgctg ggcgcgcaat catcccacgg ggtgggcccg gggcggctac catcgggatg
    18541 tgacgcgact ttccgcgctc ctcgccgtcg ccgcgctcgc cggagggccg ctcgcggggg
    18601 cgggcgcccg cccggccccg cccgaggtcg tcgccgcacc ggacggcctc gcgttcgccc
    18661 tcgcgctcgg cggcggcgga caggtcctcg cctccggccc ctacctcctc cgggtccccg
    18721 gagcggaagg gcctcttcgc ctcgagatgg cccgcctcgc ggtcgacggg gcccggctcc
    18781 ggggcgagct ccggctgtcg aacgactctg ggtcgcttct ctcgggcctc gagctcgact
    18841 tccgcagcgt cacgccgacg gtgcgggcgg tgccgggccg ggccgccccg gagccgctcc
    18901 ccctggccct cccgcgcccg ctctctgtcg gcgagctgct gccgggcgaa gcgacgccgt
    18961 tcgtcccgtt cgagatcgcg ccgctccctc tcggcgagga cgtgaccgtc gtcgtcctcc
    19021 tcggcgccgt gaccggcctc gccgtgtcgc cgccgatcgc cgtcgaggga gcccttcggc
    19081 cgaccgccct cgacacggac cgctccggcc gcctctacgt cgccacggcg ggcgtcgggc
    19141 gggtcctccg gctgaccgcc gcctcggcgg cgacacccgc cgaggcggcc cgcccgtcgg
    19201 ctccgcccac ggggatggcg ctccgccggc gcgaccacgg tctcttcgtc gcaacgggcg
    19261 gagcgacggt cgaggtctat cgcccgggcc gcacgcgccc ggcgacgctc gacgcgggac
    19321 ggcccgtcac gctcctgcgc gtcgacgcga gcggcgtcct gcgggccgcg tcggggaacg
    19381 gggtcctcgc gttcgacgag gcgaaggccg gcccgcccag gcctctcggc cccgagggga
    19441 gcgaggtcct ctccttcgac gccgacgccc gcggccgcgt ccgggccgtc gtccggaacg
    19501 gagagacgcg gcgggtcgtc gcctcggagg cagcgggcgc ttctcccctc gcggcgggcc
    19561 ggggccctgg ggccgacgcc ctcgacgcgc cccgggccgt ccgcttcgcc gcggacggcg
    19621 ccctctggat cgcggccgac ggcccgacgc cggaaagcgc cgtcctcacg cgcttctccc
    19681 cgaacgggga gccgcgcggc acgctccccc ggctcggtct cgcgctcctg ctcggcaagg
    19741 acgaggaggc gggcgtcccg ccggccgtcg acctcgcgcc cggaccggac gggcttctct
    19801 gggtccttct cgagagcggg gcgctcttcg gcgcccgccc cttctgagcg gctcgcgccg
    19861 cgggaccgcc cttcaggggc gcggcgctgc cgggaccagc ttcgagagga ggcagaagtc
    19921 ggcccggtag agggcctgtc cggcctggtt cgtcccggcg ggaacgagga agaggtcgcc
    19981 gacgcgccgg ccccgcgtcc cgacgaggcg ccacgtcccc tcggcgaggc ggtacggacc
    20041 gtcggctgag aagaggagcc ggaactgctc cacgtccgtc ccggacttca gctcctccac
    20101 ccgctcgagg aggagcgtga cggtcgcgcc ctcggcgctc cgcgccgtga acgtctcgcc
    20161 cgcggcggat cgcagcccga ggagcccctc ctccaccttc gcccgggggg cggcgagcgc
    20221 ctccgccggc aggagcgtca gccccgcgag gcccgcggcc gcgccggaaa ggaacgactt
    20281 ccgtgtgatc tccatggcac tcctcacgct acgcgcgccg cgccggcgag gccgccgcgg
    20341 cctccccgtt cccgggcgct ggacgaagcg cccggccgcg ggcatcctcc cgccgggcgg
    20401 agggacccgt gaccgacacg ctctcgattt ccgatttcgc gccgctcgtc ggcgcggcct
    20461 tcacgattcg attccccgac ggaacgctcg agctgacgct cggcaccgtg gagcccctcg
    20521 ggtcgcgcgc gccgcgagcc gacgtgccgg cgctccgcac cgagcccttc tcgctcgtct
    20581 tcctcggccc gctcgcgccc gttctccctc agcgcacctg ggcgctcgag caccccgtgc
    20641 tcggcacgca cgaagtcttc ctcgtgccgc tcggcccgca ggaaggccgg atgcgctacg
    20701 aggcggtctt caactgaccg catcgccgct ccacgacagg aaaaggtaga cccccttgtc
    20761 ctcgacgagc cggaacccga gccgctcgta gaggcggagg gcggggttca tccgctcgac
    20821 gtggatcgtg acggacttgc cggccgccga cgcccgcgcc tgcacgtccc ggacgagact
    20881 cgtcccgatc ccgccgcgcc gcgcctgggg caggagcgcc acgtcgacga tcctcacctc
    20941 cgaaggtccc tcccagaggt agagccggcc cacgtcgcgc ccgtcggcga cgacgacctc
    21001 gaacgaggcg ccgtcgtagt tcgcccgcca ccaggcgtcc tgcgcgtcga actgctggcg
    21061 gaggaacgcc tccttcgtct cgggaggcca tggcaccggc gcgagctcct cctcgcgcgt
    21121 cgacgcgtag acgcgaaaga ggagctcccg gtccgcgggc tccgcgggac ggagcgcgac
    21181 gcggcgcgcc tcggccgatt cttccgccgc cacgcccgtc accgccgcct tcgcgcccgt
    21241 cacgcgcccc tcaggtccgt ggcgggaaga cgccctgcag ggcgat
//
LOCUS       DAOONL010000060        20769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2525_length_20769_cov_22.815777, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000060 DAOONL010000000
VERSION     DAOONL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_20769_cov_22.815777"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /gene="purS"
                     /locus_tag="PLP50_10260"
     CDS             complement(<1..202)
                     /gene="purS"
                     /locus_tag="PLP50_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPA51971.1"
                     /translation="MKVQVTVVPKSGVLDPQGKAIAGALARLGFPGVGDVRAGKVFRV
                     EVEANDAAEAKALAEQMAVKLLC"
     gene            complement(279..1061)
                     /locus_tag="PLP50_10265"
     CDS             complement(279..1061)
                     /locus_tag="PLP50_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA51972.1"
                     /translation="MIEIAFLGSGSTGNCAVVRSGRTTLLLDAGLSPRQISVRLGKLG
                     ASLDDVSALLLTHEHSDHVSAAAALATKRGLPVCGTAGTLAKAGLPGPLFADLRVVRD
                     GDELVFGNGDLTVRVTATPHDGVEPCCYVFASGSGHRAGVATDLGHLSKKVLAALAGC
                     EVLGLEANHDVDVLREGAYPAFLKKRILSDVGHLSNDAAADGLERLVGERTRTLVGLH
                     LSQNNNTPTLAERALRRGLDRLRAEVALELAGPSAPSGWFRA"
     gene            complement(1058..1513)
                     /gene="dut"
                     /locus_tag="PLP50_10270"
     CDS             complement(1058..1513)
                     /gene="dut"
                     /locus_tag="PLP50_10270"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPA51973.1"
                     /translation="MSAGIAVRFRLRPECADLGPPLYASDLAAGADLRAALEVPLTLA
                     PGARSVVPTGLTLEIPPGFEAQVRARSGLALKKGLALANGVGTIDADYRGEVGVIVVN
                     LGAEPVTIERGERIAQLVVAPVVRAVFEIAPDLNGTARGAGGFGSTGTA"
     gene            complement(1510..2793)
                     /locus_tag="PLP50_10275"
     CDS             complement(1510..2793)
                     /locus_tag="PLP50_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA51974.1"
                     /translation="METRTLGDGLTVWLDPMRDVRSVALGVYVAGGSADEEPSDLGST
                     HFLEHLLFRRARRRTGADIARTTDRLGGDCDAYTTKEWMAVHARTPSERLPDALSLLL
                     DLTEAPAFTADDVETERKVILEEMAEASDVPEDRLHETFVRSYWPDHPLGAPILGTEE
                     TVGALTRARLVRRFREVFRPERTLLVAVGAFEPEAFLKLVSAERRKRRHATVPLGPAR
                     PAGPSPRGKRCAFHIPRPDLNQTHLLVGTPAPAYVDRLVPAAWLLSVVLGGGVSSRLW
                     RRVRERHGLAYQVGAGLSLHRDGGMALIEAATAPKKLPKLVKATGAVLRELRQTGVTR
                     AELRRAKDQVRAEVAMSLESTSSRRESAVRGWFYRGRPWEADESIATMEAVTAADVDD
                     AIAALFGEPDASGFGVTGPTLVGATVDDLAGELAA"
     gene            complement(2860..3450)
                     /locus_tag="PLP50_10280"
     CDS             complement(2860..3450)
                     /locus_tag="PLP50_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPA51975.1"
                     /translation="MKAGRAAGRRGRGGGYTLVALLVGMTVASILIAAVLPLASAQAQ
                     REKEAELIFRGLQYAEGIRIFRRRFGRYPNSLGELLEVKPRSVRKLWKDPMTKDGEWG
                     IVSQEGVPVVTTQTGLKGRPTPTPTPTPTPSPFKPPPGAGGGDRTGPVLGVYSKSTER
                     GYRLWEGREAYNEWKFTEASLTSSGRTTGTPGPGVF"
     gene            complement(3447..3797)
                     /locus_tag="PLP50_10285"
     CDS             complement(3447..3797)
                     /locus_tag="PLP50_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HPA51976.1"
                     /translation="MAIIGTLAAIGIPMLMNTPIKAKEAALKENLFTFRSCLDQYKAD
                     KGHYPESLEVLVQEKYIRKVPVDPFTKSASTWELVYEEPDSAEAASEEPLGIIDVKSG
                     SDGTAIDGTPYNTW"
     gene            complement(3890..4354)
                     /locus_tag="PLP50_10290"
     CDS             complement(3890..4354)
                     /locus_tag="PLP50_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPA51977.1"
                     /translation="MKAPSRVRGYSLVELVVVVTIILTLTAMIVPVARFNWTRMKEME
                     LKEALRTMRTAIDEHKRLSDQGLIPVELETEGYPKELEDLVKGIELVGQVKKTQRFLR
                     KIPIDPMTGEAEWGLRSLQDDPDSTSWGRQNVYDVYSLSESTALDGTKYKDW"
     gene            complement(4362..6758)
                     /locus_tag="PLP50_10295"
     CDS             complement(4362..6758)
                     /locus_tag="PLP50_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51978.1"
                     /translation="MTVQSTRPPRAFLLAAVLVTAFVTSGCAAERAFRQAQDHEQRRH
                     WDLAVMAYEKANELDPVNMKYQTALFRSKMQAAQTHLSRGRLHRSAGQLDLAKVELEQ
                     SVALDPTNDVALQELKRVEQEIEERRAEAAGGTPTEKAKARARGRSAAPPMLHPASDE
                     PISVSFPPDSPIKKIYQALCAASGINVIFDPQLKDDKFTIDLRNLTFQKALETAMRQA
                     GHFYKVIDERTILIAQDTQQNRKEYEDQVVRTFFLSNGDVKDVSQMVRGLLDLRRMGT
                     IPQLNAIVIRDTADKVAVAERLIEVNDKAKAEVVLDVELLQLSTSKLLELGAKLSKYE
                     TSGSLAGTATGGSGGSTSSALPWKDLLKLSLSDFNFTVPSILVNFIKSNTDTELLARP
                     QLRIAEGEKAQLVIGDRVPIPVTTINTQQAIGQSGVIPVTSYQYQDVGIKVDVEARVH
                     HNREVSLKLTVEISEVKPTASGEAAEQPTISTRTITSNIRLKDGETSFLAGLIRTKSA
                     NSRSGLPFLSDIPLIGSLFSGKRQETARDDIFLSLTPHITRAPQIDVEDLVPVWVGTE
                     NNVSFSGLNVRLESPQASDTPFDPPAEAPASRPAAPTGPVPGTMPGRAILPVQGSGPN
                     DPFRRPATPTPAPQGGSNILSGDAAQRSSGFVESGGPRSLALGLELENGELAVGETAL
                     LTLNGPAELADAGALEVTIEWDPAVAELVAVTPGPWRTGSAALNTRLDSDRGPGRVRI
                     GLGTPTGVVGLPSGALARFTLRALAPGDALVRVSAGAGLGKSGPLRPEANAVPLVVRD
                     "
     gene            complement(6769..7368)
                     /locus_tag="PLP50_10300"
     CDS             complement(6769..7368)
                     /locus_tag="PLP50_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51979.1"
                     /translation="MSALSSRKALLALLGVLLVALVYRYWPGGGTVGAGPSARGARPG
                     AAARAGEETLLPGEIPPLALGTGRGEVGGAVVRNVFAFHVPPTPTPKPLPPTPTPFPR
                     PGSVEFIGPRLPTPTPTATPIVPPAIPFRALGIFGPNDRPIVTFEDGPRLINAREGDI
                     LDGRFILRRVGRESVDFAFVGLPPEITRRIPVLPPDASR"
     gene            complement(7365..8015)
                     /locus_tag="PLP50_10305"
     CDS             complement(7365..8015)
                     /locus_tag="PLP50_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51980.1"
                     /translation="MTSSLTAFRDRRLLFALLLVVLAANAAVLLSYRTFYDDRLRALE
                     GEQAGLERRRDEARSQAERAAAAERKLFETQAALTSFFSETLGEREAKIASLIEELYA
                     TTRAVGLRPDTIGYQSVDEPGTDSLTMSFGVEGPYRDVKRLLAELESSKRFLVVERVG
                     LSGGSSDDPDSVRVRLTVTNYFRPGSLRPIRTARDARAGTAGRRPAAATRGEGATR"
     gene            complement(8012..8632)
                     /locus_tag="PLP50_10310"
     CDS             complement(8012..8632)
                     /locus_tag="PLP50_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51981.1"
                     /translation="MAAPRPNLARRPFVDTRPANLVAVLLLAAAIGLTFVSVRTVRAY
                     VEGSRTSREAIAGLRSEITRHEEARRSAETKLARFDLAGMKAGSEEANALARLRTFSW
                     TRFLTRLEKTLPNDVRVVSMGLSREAEKKRSGEARAEAESGSFSLSLRLVSRDPDGMP
                     KLIRTFYASPWFDAPTPVSEAGGEQGSVEGRTLAINVFYRDREVKP"
     gene            complement(8635..9597)
                     /locus_tag="PLP50_10315"
     CDS             complement(8635..9597)
                     /locus_tag="PLP50_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51982.1"
                     /translation="MSTDGLLARLGSALVGSWRYRRPPHAFVLTEERLVHVASPREAR
                     AGGGLAVRSRDLPPGTFLAAPGGARVAGPGLAQAVAALLPPNERLAAASLAVPDAFVK
                     AAPVDVEPGVEKNSKELAEVLRWKVGRLWGEPAPALRIGWCRAGQGAGGAPRYLVVSA
                     AEEAVASCEAAFGAHGIRIGALEPASLALSALAAKALPASGFLVFADGTTVSTVWLEG
                     GAPRFLRTRAASSDPDEALQEFRLAATFVVGADGEGPGPDLAADVIVVPEASELAGRL
                     RDFRRENGGKEPVSLLASLAGRGLPVRPGDTAPLVGLGLLEGAE"
     gene            complement(9587..11218)
                     /locus_tag="PLP50_10320"
     CDS             complement(9587..11218)
                     /locus_tag="PLP50_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPA51983.1"
                     /translation="MTTNDAPAVPAFPRVLDAASEESRAREVGRRLGIPYVDLWTFRV
                     DPALFKSVPLEWMLRHEFVPESEEDGVLSVVLTDPTDVVRRDELEALLKRRVRVKVGS
                     RAAMHEILQKSESTQRVLEEATEEFRMQVVREDEEGQEVLTIDRIAADASPIIKLVDS
                     VLFNAIQRRASDIHIETQENVVVIKYRIDGVLYPAMEPIDKKHHQTIVSRIKVMSELD
                     IAEKRIPQDGRFKVRVRGRTIDFRVSIMPTVHGEDVVIRILDKESANAEFKNLRLDVL
                     GMDVETMRRLRKFIREPYGMVLVTGPTGSGKTTTLYACLSEIQSVEDKIVTIEDPVEY
                     QLRGITQIPVNEKKGLTFARGLRSILRHDPDKVMVGEIRDEETAQIAVQAALTGHLVF
                     TTVHANNVVDVLGRFLNMKVELYNFVSALNCILAQRLVRKICPRCKTPMEPTKQLLDE
                     SALTPAQVADTVFYEGRGCIECNGTGFYGRMAITELLDLSDRIRGLILDRRPAAEIKK
                     AAKEEGMAFLRESALRKVFLGETTLKEINKVTFVD"
     gene            complement(11222..12457)
                     /locus_tag="PLP50_10325"
     CDS             complement(11222..12457)
                     /locus_tag="PLP50_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPA51984.1"
                     /translation="MPEFVCRIGTPDGSVVTRTVEAPDEAALRAELARQGSRLFSAKA
                     GSGRSAPVASLGARLGSLRGRGHVGVHEFLVFNQELVALLKAGLPVVSGFEILLERQE
                     NAHFKRILIDVREQLVSGVALSDAFLSHGDTFPRLYATSLKAGERSGEVEKVLRRFLD
                     YQKILGRVKRKVTGAMAYPIVLVSLAVGLIFILMTYVIPKFTEFYAGFGADMPALTVA
                     VVGTATFMRAHLLEILLGIGAAVFAFDRWRRTDSGRRSIDGFLLKLPAVGVVIHEFSL
                     SQFARALATLVGAGTPLVTSLEISTGSIGNRRVSDAVASVVPKVREGAELWRSLEQTG
                     QFTSLAVEMIKVGEATGALEEMLTNVSDFYDEAIEARLQTIINLIEPVILIVMGGVIA
                     TILLSVYLPMFTILSSIRG"
     gene            complement(12457..13203)
                     /locus_tag="PLP50_10330"
     CDS             complement(12457..13203)
                     /locus_tag="PLP50_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPA51985.1"
                     /translation="MRSSSLPGLLAAALFLAAPAVARTPAPKPTAGPDVVRVFVLKHR
                     KAEDAFLLVRPLLGARGTIVLQTATNALTVRDTVRTVERVARAVSAFDLPPRAIAVSV
                     SLIRATHAPGPGGPAVPVDERFRGVADRLRSLFSFTEYASLDEVVLQGLEGDPVSWTL
                     GGSFRIDFLLEAGGGQESVRLRNLVLARLRRDEAGIETARDIARTSLNLRMREPFVLG
                     IGREEGGSAALFLVLTADPVESGPGIAGRR"
     gene            complement(13203..13742)
                     /locus_tag="PLP50_10335"
     CDS             complement(13203..13742)
                     /locus_tag="PLP50_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51986.1"
                     /translation="MRELACDRLDDLLARREEDRLTAAEEGFLRGHLATCEACAAEAL
                     RRDPVLLFARAAAPEPLSAEARGRFVSGVLAATATAKAGRRLQRAHARPVLRLAASLL
                     LAASVAGVWLARDGGDGAGEVPRPELVAAADEGIRTPPDVVPAVEDLGGDTAVVYQFP
                     STRPGEPTVVFVVDRNADI"
     gene            complement(13739..14395)
                     /locus_tag="PLP50_10340"
     CDS             complement(13739..14395)
                     /locus_tag="PLP50_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA51987.1"
                     /translation="MAMQLVHVRRAPQEGTTIEPRDERHLAALAKAGDEDAFAELVRG
                     RTDSVVAFLRRMLGDTEDARDVAQLTFLRVWENLPRYDPSYAFSTWLFRIAGNLAIDA
                     LRSRGTRARTLAESFRVVQGGLLAASPEAPLRLSQEEVRRVFEACSSVLSEKQRLVFV
                     LREMEEKESREVAEIVGCRESTVRNHLFQARRLLREELKRRFPEYVPESHRSAEGAGA
                     "
     gene            14533..15762
                     /locus_tag="PLP50_10345"
     CDS             14533..15762
                     /locus_tag="PLP50_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPA51988.1"
                     /translation="MTRTDHPSAGAAGLTALLARRLLPEAAPARADVWVQGVSVGEVE
                     LAHTLALGLRAARPQTSLLVTSSTPAGVGLLGRRFRDLVPPARLQPFPLDLPVSVRRL
                     LDSAAPRLLVLMETELWPVLLRAARRRGLPVLVASGRLSERSVRRLRAARPLFRGSLA
                     ALSAVAARSEEDAARFAAVGVPEERVHVTGDMKFDRPLPPEPAFADSLRALAAGRPIL
                     VAGSIAEEELDLALSLPRALSDGPSGAPFLLLAPRRTESWDEAERRAVAAGLRIVRRS
                     RPGSGGPADVFLLDTIGELASAYRLGDIALLGGTFGRRGGHNVLEPLLASLPVVLGPS
                     VWNIRRTVEAAGAAVVAVPDAEAVAPALARLLDDPAARETARDAANALFAKHRGATER
                     TVKLALGLLGNPPGETP"
     gene            15759..16784
                     /gene="lpxK"
                     /locus_tag="PLP50_10350"
     CDS             15759..16784
                     /gene="lpxK"
                     /locus_tag="PLP50_10350"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPA51989.1"
                     /translation="MTRLLLPFSGLYGAGVSLRLALYRRGLLAVRAAARPVVSVGNVA
                     AGGTGKTPFVRWLASELLRRGRRPSILTRGYGRKSRGTVVVSDGRGTLASVSDSGDEP
                     ALLARALPAVPIVADVRRRAAAARAETLGTPVDLHLLDDGFSHVALARDVDIVLLDAT
                     APDAGGALLPTGRLREPLSSLARADLLVVTKTEQADAAPALAIARQFAPGVPVYRART
                     EVLGVIGRDGSSVEPRDLPPGTTVAVAGIARPDSFRATLAGLEIEPAELLSFRDHEPY
                     GPASLGRIERALEESGATAVVTTEKDAVKLDVLLRVPVFRVAVEAQPVEPSFVSDLLS
                     LLARRPS"
     gene            16787..17689
                     /locus_tag="PLP50_10355"
     CDS             16787..17689
                     /locus_tag="PLP50_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA51990.1"
                     /translation="MAGKHGALRDGVELGAFLVLRAFFRLLPLPLADAVGRGLGRFYR
                     LVDRRRRRLVARNLALAFPERTVAEREEISRAVFAHFGAIAADLVRSEAAPTEELLAR
                     VEVEGLEHARAAAASGRGVFFATPHLGNWEWANLVTGANGMPVTIVARPLDNPLLDAR
                     LTALRERTGGHVVNKKDAARTILRSLRAGAVVGILGDQRARPPDAVLTPFFGRLAWTT
                     TAIARLADRTGALILPVVCLRVGPGRYRLTYSEPIDPAALSPEEREVSRLTARVTELI
                     ESQVRAAPAQWLWLHDRWKGAPEN"
     gene            complement(17975..18562)
                     /locus_tag="PLP50_10360"
     CDS             complement(17975..18562)
                     /locus_tag="PLP50_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPA51991.1"
                     /translation="MVLDAPVLVLNRSFAALALTDARRGFTLFYKGHVRAVLPDFTTY
                     GWDEWCDIPVQPGDEDVVKTPGLLLKVPRVIQLLSCDRPPRYDVKFSRHNIYVRDGTR
                     CQYCGRRFPTAELSLDHVVPLSRGGVSSWENVVCACLRCNVKKGNRLPDEAGMALRRP
                     PVKPKASPLGLLSPSRIHPAWRSFLDVAYWNVELG"
     gene            18586..19593
                     /gene="waaC"
                     /locus_tag="PLP50_10365"
     CDS             18586..19593
                     /gene="waaC"
                     /locus_tag="PLP50_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HPA51992.1"
                     /translation="MKILLVRLSSFGDVVFTLPLAKALRGERDRLAWAVEGPLAGLLE
                     GAPYVDATFAATTRAWRKRPFAPESRGELRAFLASLRAFEPDVVVDAQGLLKSAWVTL
                     AARAERKVGFGFRTATEKVNPLFTRERVDVPPGAHVVDRGLALAERVLGRRGFERTPD
                     VSHLVGKPAPEVDAWLAARPARPFALLQPFSSRATKEWSPAETAAFCRALAARGIDAV
                     LRWGPGERERAEGIASLADGAALLAPPTPPAASARLAARAALFVGADTGPTHLAAAAG
                     TPTVALFGPTDPTRFGPVGPRVRILRDGSGAYNAGRPGLPGLTHDAVLEASLALLDER
                     T"
     gene            19547..20440
                     /locus_tag="PLP50_10370"
     CDS             19547..20440
                     /locus_tag="PLP50_10370"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPA51993.1"
                     /translation="MPYSKLPSRSSTRGPEPRPGRRGGAFLLALLLGGPLAASVTANV
                     LPNLGRAELPKDQVASVPFRAGETLVYTVSWLKIEGGEMTLATSREWSPDGVPVHRIR
                     LSAVSNDYVSRFYPVDTRYESWIDARDFMPVRFEKRAREGRYSADEVEEFDLAKRVGT
                     WRNAKDRRRVVALPPRFQDVISSFYFMRTVPLVPGEETRVDLCSRGKLYRLVVAVLGR
                     EKVETELGTFDAIKVQPRMHEGEGSEDQNKGKLFLWFSDDERRLPVMARTILPIGSVT
                     ARLTRVTGEPPASAAPPPAPR"
     gene            20487..>20769
                     /locus_tag="PLP50_10375"
     CDS             20487..>20769
                     /locus_tag="PLP50_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HPA51994.1"
                     /translation="MAVKLGELLLKAKLINAEQLAAALKQQKESGVKLGETLVRLGFV
                     TEDDITETLSAQFGVPSINLTHFEIDPNVLKLVPAEVARKYNILPVNKTG"
BASE COUNT         2778 a         7446 c         7594 g         2951 t
ORIGIN      
        1 tgcagaggag cttgacggcc atctgctccg cgagcgcctt cgcctcggcg gcgtcgttcg
       61 cctcgacctc gacccggaag acctttcccg cccgcacgtc gccgacgccc gggaagccga
      121 ggcgcgcgag cgcgccggcg atcgccttcc cctgcgggtc gaggacgccg gacttcggga
      181 cgaccgtgac ctggaccttc atgcgttcct cctggggcgg tcggccgatc tcggcccccc
      241 ccgatcgtcg tgcgcgccgc gcgcggccgg gccgcgcctc acgcccggaa ccacccgctc
      301 ggggcggacg gccccgcgag ctcgagggcg acctccgccc ggagccggtc gagaccccgg
      361 cggagcgcgc gctccgcgag ggtcggcgtg ttgttgttct gggagaggtg gaggccgacg
      421 agcgtccgcg tccgctcgcc gacgaggcgc tcgagcccgt cggccgccgc gtcgttcgag
      481 aggtgcccca cgtcggagag gatccgcttc ttcaggaagg ccggataggc cccttcccgg
      541 aggacgtcga cgtcgtggtt cgcctcgaga ccgagcacct cgcacccggc gagggccgcg
      601 aggaccttct tcgagaggtg gccgaggtcg gtcgcgacgc cggcgcggtg cccggagccg
      661 ctggcgaaga cgtagcagca cggctcgacg ccgtcgtgcg gcgtcgccgt gacgcgaacg
      721 gtgaggtctc cgtttccgaa cacgagctcg tcgccgtcgc ggacgacgcg taggtcggcg
      781 aagagggggc ccgggaggcc cgccttcgcg agggtgccgg cggtgccgca gaccgggagg
      841 ccccgcttcg tcgcgagggc cgctgccgcg gagacgtggt cggagtgttc gtgcgtcagg
      901 aggagggccg agacgtcgtc gagcgaggcg ccgagcttcc cgagccgaac cgagatctgc
      961 cgcggcgaga ggccggcgtc gaggaggagc gtcgtcctcc cggaacggac gacggcgcag
     1021 ttgcccgtcg agcccgatcc gaggaaggcg atctcgatca cgccgtcccc gtcgagccga
     1081 agccccccgc gccgcgggcg gttccgttca ggtcgggtgc gatctcgaag acggcgcgga
     1141 cgaccggcgc gacgacgagc tgggcgatcc gctcgccgcg ctcgatcgtg accggctcgg
     1201 cgccgaggtt cacgacgatc acgccgacct cgccgcggta gtcggcgtcg atcgtcccga
     1261 cgccgttcgc gagcgcgagc cccttcttca gcgcgaggcc ggaacgggct cgcacctgcg
     1321 cctcgaagcc gggcgggatc tcgagcgtca gaccggtcgg gacgacggag cgcgcgccgg
     1381 gagcgagcgt cagcggcacc tcgagcgccg cgcgcaggtc ggccccggcg gcgaggtcgc
     1441 tcgcgtagag cgggggaccg aggtccgcgc attccggacg tagccggaag cggacggcga
     1501 ttccggcgct cacgccgcga gctcgccggc gaggtcgtcg acggtggcgc cgacgagcgt
     1561 tgggccggtg acgccgaagc cggaggcgtc gggctcgccg aagagggcgg cgatcgcgtc
     1621 gtcgacgtcg gcggcggtga cggcctccat cgtggcgatc gactcgtcgg cctcccaggg
     1681 ccgcccgcgg tagaaccagc cgcggacggc cgactcgcgc cgagaggagg tcgactcgag
     1741 cgacatcgcc acctcggcgc ggacctggtc cttcgcgcgg cggagctcgg cgcgcgtgac
     1801 gccggtctgc cggagctcgc ggaggaccgc gcccgtcgcc ttcacgagct tcgggagctt
     1861 cttcggcgcc gtggcggcct cgatgagcgc catcccgccg tcccggtgga gcgagaggcc
     1921 cgcccccacc tggtaggcga ggccgtgccg ctcgcggacg cgccgccaga ggcgcgagga
     1981 gacgccgccg ccgaggacga cggagaggag ccacgccgcc gggacgagcc ggtccacgta
     2041 ggccggtgcg ggcgtgccga cgaggaggtg cgtctggttc aggtcgggcc tcgggatgtg
     2101 gaaggcgcag cgcttcccgc gcggagaggg cccggccggg cgggccgggc cgagcgggac
     2161 cgtcgcgtgc cgcctcttcc tccgctccgc cgagacgagc ttcaggaacg cctccggctc
     2221 gaacgcgccg acggcgacga ggagcgtccg ctccgggcgg aagacctcgc ggaagcggcg
     2281 gacgagccgg gcgcgggtga gggcgccgac cgtctcctcg gtcccgagga tcggggcgcc
     2341 gagcgggtgg tcgggccagt aggagcggac gaacgtctcg tggaggcggt cctccgggac
     2401 gtcgctcgcc tcggccatct cctcgaggat caccttccgc tccgtctcga cgtcgtcggc
     2461 ggtgaaggcg ggagcctcgg tcaggtcgag gaggagcgag agcgcgtcgg ggagccgctc
     2521 ggacggggtc cgggcgtgga cggccatcca ctccttcgtc gtgtaggcgt cgcagtcgcc
     2581 gccgaggcgg tcggtcgtcc gggcgatgtc ggcgcccgtg cggcgccggg cacgccggaa
     2641 gaggaggtgc tccaggaagt gggtcgagcc gaggtcggag ggctcctcgt cggccgagcc
     2701 tcccgccacg tagacgccga gcgcgacgct tcgcacgtcg cgcatcgggt cgagccagac
     2761 ggtcaggccg tcgccgaggg tccgcgtttc cacggcgcgg atcttacccg cgcgccccgg
     2821 aggtcatggc gtaccgcggc cctggggggc ctggccgccc tagaagaccc caggccccgg
     2881 cgtgccggtc gtcctaccgg acgaggtcag gctcgcctcc gtgaacttcc actcgttgta
     2941 ggcctcgcgc ccctcccaga gccgatagcc gcgctccgtc gacttcgagt agacgcccag
     3001 caccggtccc gttcgatccc cgccaccggc gcccggcggc ggcttgaacg ggctcggagt
     3061 cggcgtcgga gtgggcgtcg gcgtcggccg tcccttcagc cccgtttggg tagtaacgac
     3121 cgggaccccc tcctgcgaga cgattcccca ctcgccgtcc ttcgtcatcg ggtccttcca
     3181 gagcttccgg acgctccgcg gcttcacctc gaggagctcg cccagtgagt tcggataccg
     3241 tccgaagcgg cggcggaaga tccggatccc ttccgcgtac tgaaggcccc ggaagatgag
     3301 ctcggcctcc ttctcgcgct gggcctgggc ggaggcgagc gggaggacgg ccgcgatgag
     3361 gatcgaggcg accgtcatcc cgacgaggag cgccacgagc gtgtagcccc cgccgcgccc
     3421 ccggcgcccc gccgcccggc cggccttcac cacgtgttgt acggcgtccc gtcgatcgcg
     3481 gtcccgtcgg agcccgactt cacgtcgatg atccccagcg gctcctcgga ggcggcctcg
     3541 gccgagtcgg gctcttcgta gacgagctcc cacgtgctgg ccgacttcgt gaacgggtcg
     3601 acggggacct tccggatgta cttctcctgg accaggacct cgagcgactc gggatagtgc
     3661 cccttgtcgg ccttgtactg gtcgaggcag gagcggaacg tgaagaggtt ctccttcagc
     3721 gccgcttcct tcgccttgat cggcgtgttc atcagcatcg ggattccgat cgccgcgagc
     3781 gtcccgatga tcgccatgac gacgagcaac tctatgagcg tgaatccacg gcccctggag
     3841 ggctggaggg gaggctgagg ggcgagagtc tcttcagggg gcatcgtcgt caccagtcct
     3901 tgtacttcgt cccgtcgagc gccgtactct ccgagagaga gtagacgtcg tagacgttct
     3961 gtcgacccca gctcgtcgag tcggggtcgt cctggaggct ccggaggccc cattccgcct
     4021 ccccggtcat cgggtcgatc gggatcttcc gaaggaaccg ctgtgtcttc ttgacctgcc
     4081 cgacgagctc gatccccttc acgaggtcct ccagctcctt cgggtagcct tcggtctcga
     4141 gctcgaccgg gatgagcccc tggtccgaga ggcgcttgtg ctcgtcgatc gccgtgcgca
     4201 tcgtccgaag cgcctccttc agctccatct ccttcatccg cgtccagttg aatcgcgcca
     4261 ccggcacgat catcgccgtc agcgtcagga tgatcgtgac gacgacgacg agctcgacga
     4321 gcgagtatcc gcggactcgg gacggggcct tcacggcgtc ctcagtccct cacgacgagg
     4381 ggcaccgcgt tcgcctcggg gcgaagcgga ccgctcttgc cgagccccgc gccggcggag
     4441 accctcacga gggcgtcgcc cggggcgagc gcgcggagcg tgaagcgggc gagcgcgccg
     4501 ctcgggaggc cgacgacgcc ggtcggcgtg ccgaggccga tccgcacgcg ccccgggccc
     4561 cggtcggagt cgaggcgcgt gttcagcgcc gccgaaccgg tgcgccacgg tccgggcgtc
     4621 accgcgacga gctcggccac ggccgggtcc cactcgatcg tcacctcgag cgcgccggcg
     4681 tcggcgagct ccgcggggcc gttgagcgtc aggagggcgg tctcaccgac cgccagctcg
     4741 ccgttctcga gctcgaggcc gagggcgagg gaccgcggcc cgccgctctc gacgaagccc
     4801 gacgaacgct gcgccgcgtc accggagagg atgttggagc ctccctgagg cgccggagtc
     4861 ggcgtcgcgg gtcggcggaa cgggtcgttc gggccgctcc cctggacggg gaggatcgcc
     4921 cggcccggca tcgtccccgg gaccggaccc gtcggcgcgg cgggacggga ggcgggagcc
     4981 tccgcgggcg ggtcgaacgg cgtgtcggac gcttgcggcg actcgagcct cacgttgagg
     5041 ccggagaagg agacattgtt ctccgtcccg acccagacgg ggaccaggtc ctccacgtcg
     5101 atctgcggtg cgcgggtgat gtggggcgtg aggctcagga agatgtcgtc ccgggccgtc
     5161 tcctgcctct tgccgctgaa gaggctcccg atgaggggga tgtccgagag gaacgggagc
     5221 ccggaccggg agttcgccga cttcgtccgg atcaggccgg cgaggaagct cgtctcgccg
     5281 tccttcagcc ggatgttcga cgtgatcgtc cgcgtgctga tcgtcggctg ctccgcggcc
     5341 tctcccgagg cggtcggctt gacctcggag atctcgaccg tcagcttgag cgagacctcg
     5401 cggttgtggt ggacccgcgc ctcgacgtcg accttgatgc cgacgtcctg gtactggtac
     5461 gaggtgaccg ggatgacgcc ggactggccg atcgcctgct gcgtgttgat cgtcgtgacc
     5521 gggatcggga cccggtcgcc gatgacgagc tgcgccttct ccccctcggc gatccggagc
     5581 tgcggccggg cgaggagctc ggtgtcggtg ttcgacttga tgaagttgac gaggatcgac
     5641 gggacggtga agttgaagtc ggagagggag agcttgagga ggtccttcca cgggagggcc
     5701 gacgaggtcg acccgccgga gcccccggtc gccgtcccgg ccaacgaccc gctcgtctcg
     5761 tacttcgaga gcttcgcgcc gagctcgagg agcttcgacg tgctcagctg gaggagctcg
     5821 acgtcgagga cgacctcggc cttcgccttg tcgttcacct cgatgagccg ctcggcgacg
     5881 gcgaccttgt cggccgtgtc gcggatgacg atcgcgttca gctgcgggat cgtccccatc
     5941 cggcgcaggt cgaggagccc ccggaccatc tgggagacgt ccttcacgtc gccgttcgag
     6001 aggaagaagg tccggacgac ctggtcctcg tactccttcc ggttctgctg ggtgtcctgc
     6061 gcgatgagga tcgtcctttc gtcgatcacc ttgtagaagt gccccgcctg gcgcatggcc
     6121 gtctcgagcg ccttctggaa cgtcaggttc cggaggtcga tcgtgaactt gtcgtccttc
     6181 agctgcgggt cgaagatgac gttgatcccg gaggcggcgc agagcgcctg gtagatcttc
     6241 ttgatcggcg agtcgggagg gaacgacacg ctgatcggct cgtcggaggc ggggtggagc
     6301 atcggcgggg cggccgaccg cccccgcgcc cgcgccttcg ccttctccgt cggcgtcccc
     6361 ccggccgcct cggcccggcg ctcctcgatc tcctgctcga cccgcttcag ctcctggaga
     6421 gcgacgtcgt tcgtcgggtc gagcgcgacg ctctgctcga gctccacctt cgcgaggtcg
     6481 agctgcccgg ccgagcggtg gaggcgcccg cgcgagaggt gcgtctgggc cgcctgcatc
     6541 ttcgagcgga agagcgccgt ctggtacttc atgttgacgg ggtcgagctc gttcgccttc
     6601 tcgtacgcca tgacggcgag gtcccagtgc cggcgctgct cgtggtcctg cgcctggcgg
     6661 aaggcgcgct cggcggcgca tcccgacgtg acgaaggccg tgacgaggac ggcggcgagg
     6721 aggaaggcgc ggggcggtcg cgtgctctga acggtcatgg gcttctcttc agcgggaggc
     6781 gtcgggcgga aggacgggga tccgccgggt gatctcgggc ggcagcccga cgaaggcgaa
     6841 gtcgaccgac tcgcggccga cgcgccggag gatgaagcgg ccgtcgagga tgtctccctc
     6901 gcgggcgttg atgaggcggg ggccgtcctc gaacgtgacg atcggcctgt cgttcggtcc
     6961 gaagatcccg agcgcgcgga acgggattgc gggagggacg atcggcgtcg ccgtcggcgt
     7021 cggcgtcggg aggcgcggcc cgatgaattc gaccgagccg ggcctcggga acggcgtcgg
     7081 ggtcggcggg agcggtttcg gagtcggcgt gggcgggacg tggaacgcga agacgttccg
     7141 gacgaccgcg cctccgacct cgccgcgccc cgtcccgaga gcgaggggcg ggatctcacc
     7201 cgggaggaga gtctcctccc cggcccgagc ggcagcgccg gggcgggcgc ctctcgccga
     7261 gggaccggcg ccgaccgtcc cgccacccgg ccagtagcgg tagacgagcg cgacgaggag
     7321 gacacccagg agcgcgagga gcgccttacg ggaggagagg gcgctcatcg ggtcgccccc
     7381 tctccacggg tcgcagcggc cggccgccgc cccgccgttc cggcgcgggc gtcccgggcg
     7441 gtccggatgg gtcgcagcga gccgggccgg aagtagttcg tcacggtgag tcgtacgcgg
     7501 accgagtccg gatcgtccga cgagccgccg gagaggccga cccgttcgac gacgaggaaa
     7561 cgcttcgagc tctccagctc ggcgaggagc ctcttcacgt cgcggtacgg cccctcgacg
     7621 ccgaacgaca tcgtgaggga gtcggtgccc ggctcgtcga ccgactggta cccgatcgtg
     7681 tccgggcgca ggccgaccgc gcgggtcgtc gcgtagagct cctcgatcag ggaggcgatc
     7741 ttcgcctcgc gctcgccgag cgtttccgag aagaacgacg tgagagcggc ctgcgtctcg
     7801 aacagcttcc gctcggcggc cgcggctctc tccgcctggc tgcgggcctc gtcgcggcgg
     7861 cgctcgaggc cggcctgctc tccttcgagg gcgcgaaggc gatcgtcgta gaacgtccgg
     7921 tacgagagga ggaccgcggc attcgccgcg aggacgacga ggaggagagc gaagaggagt
     7981 cgccggtccc ggaaggcggt cagcgagctc gtcacggctt cacctcacgg tcgcggtaga
     8041 agacgttgat cgcgagcgtc cgcccctcga cgctcccctg ctcgccgccc gcctcgctga
     8101 ccggtgtcgg cgcgtcgaac cagggcgagg cgtagaaggt ccggatcagc ttcggcattc
     8161 cgtcggggtc gcgagagacg aggcggaggg agagcgagaa ggacccgctc tccgcctccg
     8221 ctctcgcctc gccggagcgc ttcttctccg cctcgcggga gagccccatg gagacgacgc
     8281 gcacgtcgtt cgggagagtc ttctcgaggc gcgtcaggaa ccgggtccag gagaacgtcc
     8341 ggaggcgcgc cagggcgttc gcctcctccg atccggcctt cattccggcg agatcgaacc
     8401 gggcgagctt cgtctcggcc gaccggcggg cctcctcgtg acgggtgatc tcggagcgca
     8461 gccccgcgat cgcctcgcgc gacgtccggg aaccttcgac gtaggcgcgg accgtccgga
     8521 cgctcacgaa ggtcaggccg atcgccgcgg cgagaaggag gacggccacg aggttcgcgg
     8581 gccgggtgtc gacgaaggga cggcgggcga ggttcggtct cggggcggcc atggtcactc
     8641 cgctccttcg aggagcccga gcccgacgag aggcgccgtg tctccggggc ggaccggaag
     8701 cccccggccg gcgagggacg cgaggaggga gacgggctcc ttccctccgt tctcgcgccg
     8761 gaagtcccgg agccggccgg cgagctccga cgcctcgggg acgacgatca cgtcggccgc
     8821 caggtcggga ccggggccct cgccgtcggc cccgacgacg aacgtcgccg cgagccggaa
     8881 ctcctggagc gcctcgtcgg ggtccgacga ggcggcgcgg gtccggagga accggggcgc
     8941 cccgccctcc agccagacgg tcgagacggt cgtgccgtcg gcgaagacga ggaagccgga
     9001 ggccgggagc gccttcgccg cgagggccga gagggcgagc gaggcgggct cgagggcgcc
     9061 gatccggatt ccgtgcgccc cgaaggccgc ctcacacgag gcgacggcct cctccgccgc
     9121 ggagacgacg aggtagcggg gcgcgcctcc ggccccctgt ccggcccggc accagccgat
     9181 ccggagcgcg ggcgccggct cgccccagag ccgccccacc ttccagcgaa ggacctcggc
     9241 cagctccttc gagttcttct cgacgccggg ctcgacgtcg acgggggccg ccttcacgaa
     9301 ggcgtccggg acggcgaggg acgcggccgc gaggcgctcg ttcggcggga ggagcgcggc
     9361 gacggcctgc gcgaggccgg gccccgcgac gcgggcgccg ccgggggcgg ccaggaacgt
     9421 gcccggcggg agatcgcggg agcggacggc gaggccgccc cccgctcgcg cctcgcgggg
     9481 agaggcgacg tggacgagcc gctcctccgt caggacgaag gcgtgcggcg gccgccggta
     9541 gcgccagctc ccgacgaggg cgctcccgag gcgggcgagg agtccgtcag tcgacaaagg
     9601 tcaccttgtt gatctctttc agcgtcgtct ccccgaggaa gaccttgcgg agcgccgact
     9661 cgcggaggaa ggccatcccc tcctccttcg ccgccttctt gatctccgcg gcgggccgcc
     9721 ggtcgaggat gaggccgcgg atccggtcgg agaggtcgag gagctcggtg atcgccatcc
     9781 gaccgtagaa gccggtcccg ttgcactcga tgcagccgcg cccctcgtag aagacggtgt
     9841 ccgcgacctg cgcgggggtg agggccgact cgtcgaggag ctgcttcgtc ggctccatcg
     9901 gcgtcttgca gcgcgggcag atcttccgga cgagccgctg ggcgaggatg cagttgaggg
     9961 ccgagacgaa gttgtagagc tcgaccttca tgttgaggaa ccggcccagg acgtcgacga
    10021 cgttgttcgc gtggaccgtc gtgaagacga ggtgccccgt gagcgccgcc tggacggcga
    10081 tctgggccgt ctcctcgtcg cggatctcgc cgaccatgac cttgtcgggg tcgtgccgga
    10141 ggatggagcg caggcccctc gcgaacgtca gccccttctt ctcgttgacc gggatctgcg
    10201 tgatgccgcg gagctggtac tcgaccgggt cctcgatcgt gacgatcttg tcctcgacgg
    10261 actggatctc cgagaggcag gcgtagagcg tcgtcgtctt cccggagccg gtcggcccgg
    10321 tgacgaggac catcccgtag ggctcgcgga tgaacttgcg gagccgcctc atcgtctcga
    10381 cgtccatccc gaggacgtcc agcctgaggt tcttgaactc ggcgttcgcc gactccttgt
    10441 cgaggatccg gatgacgacg tcctcgccgt ggacggtcgg catgatcgag acgcggaagt
    10501 cgatcgtccg cccgcggacc cggaccttga agcgcccgtc ctgcgggatc cgcttctcgg
    10561 cgatgtcgag ctccgacatg accttgatgc ggctgacgat cgtctggtgg tgcttcttgt
    10621 cgatcggctc catcgccggg tagaggacgc cgtcgatccg gtacttgatg acgacgacgt
    10681 tctcctgggt ctcgatgtgg atgtcggagg cgcggcgctg gatcgcgttg aagaggacgg
    10741 agtcgacgag cttgatgatc gggctggcgt cggccgcgat ccggtcgatc gtcaggacct
    10801 cctgcccctc ctcgtcctcc cgcacgacct gcatccggaa ctcttccgtc gcctcctcga
    10861 ggacccgctg cgtcgactcg gacttctgga ggatctcgtg catcgcggcc cgcgagccga
    10921 ccttcacgcg gacccggcgc ttcaggagcg cctcgagctc gtcgcgccgg acgacgtccg
    10981 tcgggtccgt caggacgacg gagaggacgc cgtcctcctc gctttccggg acgaactcgt
    11041 ggcggagcat ccactcgaga gggacgctct tgaagagagc ggggtcgacc cggaaggtcc
    11101 agaggtcgac gtagggaatg ccgagcctcc gcccgacctc ccgggcgcgg ctctcctcgg
    11161 aggccgcgtc caggacgcgc gggaaggcgg ggacggcggg ggcgtcgttc gtcgtcatgc
    11221 gtcagccccg gatgctggag aggatcgtga acatcggtag gtatacggac aggaggatcg
    11281 tcgcgatgac gccgcccatc acgatcagga tgaccggctc gatcaggttg atgatcgtct
    11341 ggaggcgcgc ctcgatcgcc tcgtcgtaga agtccgagac gttcgtcagc atctcctcga
    11401 gggcgcccgt cgcctcgccc accttgatca tctcgacggc gagggaggtg aactgccccg
    11461 tctgctcgag gctccgccag agctcggccc cctcccgcac cttcggcacg acggaggcga
    11521 ccgcgtccga aacgcgtcgg ttgccgatgg agccggtcga gatctcgagc gaggtgacga
    11581 gcggcgtgcc ggccccgacg agcgtcgcga gggcccgggc gaactgcgag agggagaact
    11641 cgtggatgac gacgccgacc gccgggagct tcaggaggaa cccgtcgatc gaccgtcgtc
    11701 cggagtcggt ccgacgccag cggtcgaacg cgaagaccgc cgccccgatg ccgaggagga
    11761 tctcgaggag gtgcgcgcgc atgaaggtcg ccgtgccgac gacggccacg gtcagcgccg
    11821 gcatgtccgc gccgaagccc gcgtagaact ccgtgaactt cgggatgacg tacgtcatca
    11881 ggatgaagat cagcccgacg gcgaggctca cgaggacgat ggggtaggcc atcgccccgg
    11941 tcaccttccg cttcacacgg ccgaggatct tctggtagtc gaggaaacgc cggaggacct
    12001 tctcgacctc gccggagcgc tcccccgcct tgagcgaggt ggcgtagagg cgcgggaacg
    12061 tgtcgccgtg cgagaggaac gcgtccgaga gcgccacccc cgagacgagc tgctccctca
    12121 cgtcgatcag gatccgcttg aagtgcgcgt tctcctgccg ctccaggagg atctcgaagc
    12181 cggagacgac cgggaggccg gccttcagga gcgcgacgag ctcctggttg aagacgagga
    12241 actcgtggac accgacgtgc ccccggcccc gcagcgagcc gaggcgggcc ccgaggctcg
    12301 cgaccggggc gctccggccg ctccccgcct tcgcggagaa gagccgcgag ccctggcggg
    12361 cgagctcggc ccggagggcc gcctcgtcgg gggcctccac ggtccgggtg acgacgctcc
    12421 cgtcgggcgt cccgatgcgg cagacgaact cgggcatcag cgccgccccg cgatcccggg
    12481 accggactcc accggatcgg ccgtcaggac gaggaagagc gccgcgctgc ccccctcctc
    12541 gcgaccgatg ccgaggacga acggctcgcg catccggagg ttcagggagg tccgggcgat
    12601 gtcccgggcc gtttcgattc cggcctcgtc gcgccggaga cgggcgagga cgaggttccg
    12661 caggcgaacg ctctcctgcc ctcctcccgc ctcgaggagg aagtcgatcc ggaagctccc
    12721 tcccagggtc caggagaccg ggtcgccctc gagcccctgc aggacgacct cgtcgagcga
    12781 ggcgtactcg gtgaagctga agaggctccg gagccggtcg gcgacgccgc ggaagcgctc
    12841 gtccaccggc accgccgggc ctcccgggcc gggcgcgtgc gtcgcacgga tgagcgagac
    12901 ggagacggcg atcgcccgcg gcgggaggtc gaacgccgag accgcccgag cgaccctctc
    12961 gacggtgcga acggtgtccc tcaccgtcag ggcgttcgtc gcggtctgaa ggacgatcgt
    13021 cccgcgcgcg ccgaggagcg gccgaacgag aaggaacgcg tcctccgcct tccggtgctt
    13081 caggacgaag acccggacga cgtccggtcc cgccgtcggc ttcggcgcgg gggtcctggc
    13141 gacggccgga gcggcgagga agagcgcggc cgcgaggagg cccggcaggg acgacgacct
    13201 catcagatgt ccgcgttccg gtcgacgacg aagacgaccg tcggctcgcc cggccgggtc
    13261 gacgggaact ggtagacgac ggccgtgtcg ccgccgaggt cctcgaccgc ggggacgacg
    13321 tcgggcgggg tgcggatccc ctcgtccgcg gcggcgacga gctcgggacg aggcacctcg
    13381 ccggcgccgt ccccgccgtc ccgcgcgagc cagacgcccg cgacggaggc ggcgagcagg
    13441 agcgatgcgg cgagccggag gacgggacgg gcatgcgctc tctggaggcg ccggccggcc
    13501 ttcgcggtcg cggtggccgc gaggacgccg ctgacgaacc gcccccgcgc ctcggcggag
    13561 agcggctccg gcgcggccgc ccgggcgaag aggaggaccg ggtcgcggcg caacgcctcg
    13621 gcggcgcacg cctcgcaggt ggcgaggtgc ccccggagga agccctcctc ggccgcggtg
    13681 aggcgatcct cctcccgccg cgccaggagg tcgtcgaggc ggtcgcaggc gagctctctc
    13741 acgctccggc tccctcggcg ctccggtgcg actcggggac gtattccggg aagcgccgct
    13801 tcagctcctc acgcaggagg cgccgggcct ggaagaggtg gttcctcacg gtcgactccc
    13861 ggcagccgac gatctcggcc acctcgcggc tctccttctc ctccatctcg cgcaggacga
    13921 agacgagccg ctgcttttcc gacaggaccg acgagcacgc ctcgaagacg cggcggacct
    13981 cttcctggga gaggcgcaga ggcgcctccg ggctcgcggc gaggagcccc ccctgcacga
    14041 cgcggaagct ctcggcgagc gtccgggccc gcgtccccct cgagcggagg gcgtcgatcg
    14101 cgaggttccc ggcgatccgg aagagccagg tcgagaaggc gtacgacggg tcgtagcgcg
    14161 ggaggttctc ccacacgcgg aggaacgtca gctgggcgac gtcgcgcgcg tcttccgtgt
    14221 cgccgagcat ccggcggagg aacgcgacga cggaatcggt tcgcccgcgg acgagctcgg
    14281 cgaaggcgtc ctcgtcgccc gccttcgcga gcgccgcgag gtgccgttcg tccctcggct
    14341 cgatcgtcgt cccctcctgc ggcgcccggc ggacgtggac cagctgcatg gccatgccgt
    14401 cggttctacg gttccgcgcc cggcctcgtc tacgggacct ccgtctccgg ctcccgctcg
    14461 cggtggggcg cggcgcggcg catataatgc ggcctctcgg atgccgaacg taacctcttc
    14521 cagggaagcc ggttgacccg aaccgaccac ccttcggccg gtgcggccgg cctgacggcc
    14581 ctcctcgcgc ggaggctcct tcccgaggcc gcgccggctc gcgccgacgt ctgggtacag
    14641 ggggtctcgg tcggagaggt cgagctcgcc catacgctcg cgctcggact tcgggccgct
    14701 cgcccgcaga cgtcgctcct cgtcacgtcc tccaccccgg ccggcgtcgg tctcctcgga
    14761 aggcggttcc gcgacctcgt cccgccggcc cggctgcagc cgttccccct cgacctcccg
    14821 gtctcggtac gccggctgct cgactcggcc gcgccccgcc tcctcgtcct gatggagacc
    14881 gagctctggc ccgtcctcct ccgggcggcc cggcgccgcg ggctgccggt cctcgtggcg
    14941 agcgggcgcc tctccgagcg atcggtccgc cggctccgag ccgctcggcc gctcttccgc
    15001 ggcagcctcg ccgctctctc agccgtcgcg gcccgcagcg aggaggacgc cgcgcggttc
    15061 gccgccgtcg gcgtccccga ggagcgggtc cacgtcaccg gggacatgaa gttcgaccgg
    15121 cccctccctc ccgagccggc gttcgccgac tccctgcggg cgctcgccgc cggccggccg
    15181 atcctcgtcg ccggctcgat cgcggaagaa gagctcgacc tcgcgctctc gctcccgcgc
    15241 gccctctccg acggcccgtc gggagcgccg ttcctcctcc tcgccccgcg tcggacggag
    15301 agctgggacg aggcggagcg ccgcgccgtc gccgccgggc tgaggatcgt ccgaaggtcg
    15361 cgccccgggt cgggcggccc ggccgacgtc ttcctcctcg acacgatcgg ggagctcgcg
    15421 tcggcctaca ggctcggcga catcgcgctc ctcggcggca cgttcgggcg gcggggcggg
    15481 cacaacgtcc tcgagccgct cctggcgagc ctcccggtcg tcctcggccc ctccgtctgg
    15541 aacatccggc gaaccgtcga ggcagccggc gccgcggtcg tcgcggtgcc cgacgccgag
    15601 gccgtcgcgc ccgctctggc gcgcctcctg gacgaccctg cggcccggga gaccgcccgc
    15661 gacgcggcga acgccctctt cgcgaagcac cgcggcgcga cggagcggac ggtgaagctc
    15721 gctctcggcc tcctcgggaa ccctcccgga gagacgccgt gacccggctc ctcctgccgt
    15781 tctccggcct ctacggcgcc ggggtgtcgc tccgcctcgc gctctaccgg cgcggcctcc
    15841 tcgccgtccg cgccgccgcc cggcctgtcg tctccgtcgg gaacgtggcc gcgggcggga
    15901 ccgggaagac ccccttcgtc aggtggctcg cctccgagct gctccgccgc ggccggcgcc
    15961 cgtcgatcct gacgcgcggg tacggccgca agagccgcgg gaccgtcgtc gtctcggacg
    16021 ggcgcgggac gctcgcttcc gtctccgact cgggcgacga gccggccctt ctcgcccggg
    16081 cgctcccggc cgtcccgatc gtcgccgacg tgcggcggcg agccgcggcc gcccgcgccg
    16141 agacgctcgg gaccccggtc gacctgcacc tcctcgacga cggcttctcg cacgtcgccc
    16201 tggcccggga cgtcgacatc gtccttctcg atgcgaccgc gcccgacgcg gggggcgcgc
    16261 tcctcccgac cggccgcctg agggagccgc tctcgtctct cgccagggcc gacctcctcg
    16321 tcgtcacgaa gacggagcag gccgacgccg ctccggccct cgcgatcgcg cggcagttcg
    16381 ccccgggcgt cccggtctac agggcgcgga cggaagtcct cggggtgatc ggccgggacg
    16441 gctcctccgt cgagccgcgc gacctgccgc ccgggacgac cgtcgcggtc gcgggaatcg
    16501 cccgccccga ctctttccgc gcgacgctcg ccggcctcga gatcgagccg gcggagctcc
    16561 tctccttccg cgatcacgag ccgtacggcc cggcttcgct cggccggatc gagcgggcgc
    16621 tcgaggagag cggcgcgacc gccgtcgtga cgacggagaa ggacgccgtg aagctggacg
    16681 tcctccttcg cgtccccgtc ttccgggtcg cggtcgaggc gcagcccgtc gagccctcct
    16741 tcgtctccga cctcctctcg ctcctcgccc ggaggccctc gtgaggatgg ccggcaagca
    16801 cggggccctg cgagacgggg tcgagctcgg cgcgttcctc gttctccgcg ccttcttccg
    16861 gctcctcccg ctcccgctcg ccgacgccgt cggtcggggc ctcggccgct tctaccgcct
    16921 cgtcgaccgg aggcgccgcc gcctcgtcgc ccggaacctg gcgctcgcct tccccgagag
    16981 gacggtcgcc gagcgggagg agatctcgcg cgcggtcttc gctcacttcg gcgcgatcgc
    17041 cgccgacctc gtccggtccg aggcggctcc gaccgaagag ctgctcgcgc gcgtcgaggt
    17101 cgaggggctc gagcacgcgc gggccgccgc cgcctccgga cgcggcgtct tcttcgccac
    17161 gccgcacctc ggcaactggg agtgggcgaa cctcgtcacc ggcgccaacg gcatgcccgt
    17221 cacgatcgtg gcgcggccgc tcgacaaccc gctcctcgac gcgaggctca cggcgctgcg
    17281 cgaaaggacg ggggggcacg tcgtgaacaa gaaggacgcc gcccggacga tcctccgatc
    17341 tctgcgggcc ggggccgtcg tcgggatcct gggcgaccag cgggcccgcc cgcccgacgc
    17401 cgtcctgacg ccgttcttcg ggcgcctcgc ctggacgacg accgcgatcg cgcgcctggc
    17461 cgaccggacc ggcgccctga tcctccccgt ggtctgcctg cgggtcgggc cggggcgcta
    17521 ccggctcacc tactccgagc cgatcgaccc ggcggctctg tcgccggagg aaagggaggt
    17581 ctcgcgcctg acggcgcgcg tgacggagct gatcgagagc caggtccgcg cggcccccgc
    17641 gcagtggctc tggctgcacg accgctggaa gggcgccccc gaaaactgaa gaaccccggc
    17701 ggtgaggccg gggttcctga agctcgggtg ctgtggtgcg ctctctctct aggctccctc
    17761 cgctgccggc tcccgacgcg tgggtcggcc tccggcgccc gggggtactt caactttcct
    17821 gccacggcgc ccgacgggag gggagcgcag gcgcatctgc tgagattgaa aacagatacg
    17881 ctcgttgcgg cgcttcggga gaaggcgggc cggtgggccc gggtgcacga atccgtgcgt
    17941 tcccgttgaa cggaatgacg cgccggcgcc cggctcagcc gagctcgacg ttccagtagg
    18001 cgacgtcgag gaagcttcgc caggccgggt ggatccggct cggcgagagg aggccgagcg
    18061 gcgaggcctt cggcttcacc ggcggccggc ggagcgccat ccccgcctcg tcggggagcc
    18121 ggttcccctt cttcacgttg caccgcaggc aggcgcagac gacgttctcc cacgaggaga
    18181 cgccccctct cgagagcggg acgacgtggt cgagggagag ctccgcggtc gggaaacgcc
    18241 ggccgcagta ctggcagcgc gtcccgtcgc ggacgtagat gttgtgtcgg gagaacttga
    18301 cgtcgtagcg cggaggccgg tcgcaggaga ggagctggat gacccggggg actttcagga
    18361 ggaggcccgg tgtcttcacg acgtcctcgt cgccgggctg gaccgggatg tcgcaccact
    18421 cgtcccagcc gtacgtcgtg aagtcgggaa gcacggcccg gacgtggccc ttgtagaaga
    18481 gcgtgaaccc gcgtcgcgcg tccgtgaggg cgagggcggc gaacgaccgg ttcaggacga
    18541 gaaccggcgc gtcgagcacc attacgtgag aatagcgaaa cgcgcatgaa gattctcctc
    18601 gtccgcctct cctcgttcgg agacgtcgtc ttcacgctcc cgctcgcgaa ggcgctgcgc
    18661 ggagagcggg accggctcgc gtgggccgtg gaggggccgc tcgccggcct cctcgaaggg
    18721 gccccgtacg tcgacgcgac gttcgccgcg acgacgcggg cctggaggaa gaggccgttc
    18781 gccccggaga gccgcgggga gctccgcgcg ttcctcgcgt cgctgcgcgc gttcgagccc
    18841 gacgtcgtcg tcgacgcgca ggggctcctg aaatcggcct gggtcacgct cgccgcgcgc
    18901 gcggagcgca aggtcggctt cggcttccgg acggccaccg agaaggtgaa cccgctcttc
    18961 acccgcgagc gggtcgacgt cccgccgggc gcgcacgtcg tcgaccgcgg cctggccctc
    19021 gccgagaggg tcctcggccg gcgcggcttc gagcggaccc ccgacgtctc gcacctcgtc
    19081 gggaagccgg ccccggaagt ggacgcctgg ctcgccgcgc gccccgcgcg ccccttcgcg
    19141 ctcctccagc cgttctcctc gcgcgcgacg aaggagtggt cccccgccga gaccgccgcg
    19201 ttctgccgcg cgctcgccgc gcgcgggatc gacgccgtcc tccgttgggg accgggcgag
    19261 cgggaacgcg ccgagggaat cgcctccctc gcggacggcg cggcgctcct cgccccgccg
    19321 acgccgccgg ccgcctcggc gcgcctcgcg gcgcgggcgg cccttttcgt cggcgccgac
    19381 accggcccga cgcacctcgc ggccgccgcc ggcacgccga cggtcgcgct cttcggcccc
    19441 accgacccga cacgcttcgg gccggtgggg cctcgggtcc ggatcttgcg tgacgggagc
    19501 ggggcctaca atgccggtcg ccccggcctc cccggactga cgcacgatgc cgtactcgaa
    19561 gcttccctcg cgctcctcga cgagaggacc tgagccccgc cccggaaggc gcggaggggc
    19621 ctttctcctc gcgctcctgc tcggggggcc cctcgctgcc tccgtcacgg cgaacgtcct
    19681 cccgaacctc ggccgggcgg agctcccgaa ggaccaggtc gcgtcggtgc cgttccgcgc
    19741 cggcgagacg ctcgtctaca ccgtctcgtg gctcaagatc gaggggggcg agatgacgct
    19801 cgccacgagc cgggagtgga gccccgacgg ggtcccggtc caccggatcc gcctctcggc
    19861 cgtgtcgaac gactacgtct cgcgcttcta ccccgtcgac acccggtacg agtcgtggat
    19921 cgacgcgcgg gacttcatgc cggtccgctt cgagaagagg gcccgggaag ggcgctactc
    19981 ggccgacgag gtcgaggagt tcgacctcgc gaagcgcgtc gggacctggc ggaacgccaa
    20041 ggaccgtcgg cgggtcgtcg ccctcccgcc ccgcttccag gacgtcatct cgtccttcta
    20101 cttcatgagg accgtccccc tcgtgccggg ggaggagacc cgcgtcgatc tctgctcccg
    20161 cgggaagctc taccggctcg tcgtggcggt cctgggccgg gagaaggtcg agacggagct
    20221 cggcaccttc gacgcgatca aggtccagcc ccggatgcac gagggcgagg ggagcgaaga
    20281 ccagaacaag gggaagctct tcctctggtt cagcgacgac gagcggcgcc tcccggtgat
    20341 ggcccggacg atcctcccga tcggctcggt gacggcccgc ctcacccgcg tgacggggga
    20401 gccccccgcc tcggccgccc cccctccggc ccctcgctga agcgccggac ctgccggagg
    20461 gcgggcgacc gactatcatt gccctcatgg cggtcaagct cggagaactt ctcctgaagg
    20521 cgaagctcat caacgcggag cagctcgccg cggccctgaa gcagcagaag gaatcggggg
    20581 tgaagctggg cgagacgctc gtccggctcg gcttcgtcac cgaggacgac atcacggaga
    20641 cgctctccgc gcagttcggc gtgccgtcga tcaacctgac ccacttcgag atcgacccga
    20701 acgtcctgaa gctggtcccc gccgaggtcg cgcgcaagta caacatcctc ccggtcaaca
    20761 agaccggcg
//
LOCUS       DAOONL010000061        20411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2579_length_20411_cov_12.906219, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000061 DAOONL010000000
VERSION     DAOONL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_20411_cov_12.906219"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..2244
                     /gene="csaB"
                     /locus_tag="PLP50_10380"
     CDS             469..2244
                     /gene="csaB"
                     /locus_tag="PLP50_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HPA51995.1"
                     /translation="MSAPRSRVVVCGYYGFGNAGDELILAAMLRELRTQRPGLVVTVI
                     SGDPESTRASHGVDAVHWQDVPAIGARVRHADLVVVGGGGLFQEYSGIDPDSLFTDRH
                     YAITFYAESAILGALCGRPVMLYAVGVGPLFSEHGRRVVRAACDAAQVITLRDEESAR
                     VVASLGVDPERLRVTADPVFALPAGGDARRPADAARAIPPGEGPLVGVALRHWDVGVA
                     PYFWEREVAAGLDAFLESHEARLLFVPFQHLARANENDVAVAERVRSRLRKPERASVF
                     PGGGDPSALGAVLGCCDLVLGMRLHAMILAATAGVPAVALSYDPKVELAMRQLGAERF
                     VVPLADVEARTVATLMEEALAEGASRRGDLGERVGRLAEQARSNAVAAIDLLDRGARE
                     RTLSPALAEVIARSVTSRFEVEATLRGEAAALREAAGLAEAALAESGSARAAAEAAAE
                     SRAGELRRKGEELAACEAELAQARSVIGRRETELKEVHTRLMSLDTELRRSRTDLAAR
                     SAELEEVRGDLSTESAELEPALDELSRQDEALEGSRTEVRGLRDRVAGLEGDLARIHR
                     SRLWKLATRYWRFLEAIGRLPGPRS"
     gene            2413..3588
                     /gene="wecB"
                     /locus_tag="PLP50_10385"
     CDS             2413..3588
                     /gene="wecB"
                     /locus_tag="PLP50_10385"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPA51996.1"
                     /translation="MSGPGADPRAGVLVVIGTRPEAIKLAPVVEALQGSETLRPVILA
                     TSQHREMLRQALAPFDLEPDVDLEVMTAGQSPTMVAARVLDRIERSLQDIAPAFVVVQ
                     GDTTTALAAAWASFYAGIPVAHVEAGLRSGRIDSPFPEEFNRRGVSVAASLHFAPTPG
                     ARENLLGEGVSPDGVFVVGNTNVDAVARILKGRRRPEPEPGRATILVTLHRRESFGPP
                     LDRALGAIRRLALEQGDRVRVVYPVHPNPKVSEPARRHLLDLPNVELRPPLDYPEFLE
                     VFSTSRFALSDSGGVQEEAPTVRVPVLIARETTERPEVLESGWGILVGTDPDRILGES
                     RRLLDDEDAYRARCAGPNPFGDGTAARRIVDVLSTRWAARPRSPLATPELRTSRGAK"
     gene            3590..5233
                     /locus_tag="PLP50_10390"
     CDS             3590..5233
                     /locus_tag="PLP50_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51997.1"
                     /translation="MSAEDRVASLARTKAGLEADLLETSLDLRRASEDYEALAAAFRA
                     SAERLREAAGAVSAAHHEREELVRVVASLQAELAATRRDRDEVKRSLEEVCSTRVWRL
                     GTLYWRLLGRLGLLPAKAGGALAGAASVASEAAGPVADGPGQAMLAVPAAPPGVEMPT
                     RTGRSRRDPAAPGLPDVVCFSIVEWEFLFQRPQQLLSRLADLGHRVYYISQFFEPDPG
                     APEVHRLRSRVYTLRLRGSSGRLFSEELTPTDAGAVFESLASLREREGITAAVSVVHQ
                     PFWWPLAERARSAFGWAVLYDCMDDHGGFQTNARPVGGAERGILEGADTVVASSRVLE
                     SRIHAVKRPVELIPNGCDYEYFSSMAPRSRGVRPTVGYYGCIADWFEADLVADVAERR
                     PDWEFLLIGPTYLADLTRLPALPNVSFPGVVPYGRLLDRIEPIDVFLLPFRRSTLTEA
                     ANPVKAYEIMATGRPLVSVPLPELEPFGDLVRFGNGPVEFERRIEEALREDGPEIVDR
                     RRDFARRNSWDTRAARFAELVRALGAGREAGPPRPEDDA"
     gene            5230..9672
                     /locus_tag="PLP50_10395"
     CDS             5230..9672
                     /locus_tag="PLP50_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA51998.1"
                     /translation="MSSSGDERPPQGRDDGLAEVEELRALVDRSRNRLRTLRQRLRRR
                     ELQVAARSGSVEELLRRAEERGRGRMAARQESASPHGTRPAGPDASIQALTAEIASQA
                     ETIAALTRERDALGAELRGIHSSKLWRLGAAYWSVLRAVGLVKPPPGPEPGVTALPAT
                     EGTAPLGVPEPAAVPGPAVGAVVDTAAETAPVVGSRGEGDGHARPGGAAFDVVCFSII
                     EWDFRFQRPQQLTSRLADRGHRVFYVSQVFRPEGPAVTLREIRPNVWEVSLRGPAKNV
                     YRDDLAPSEVEALFESLDVLRREEGLGATVSLVQLPFWAPLVEEARSRLSWPVVYDCM
                     DFHAGFSTNESAMLSRERQLLSGADLVVVSSRFLQDEATPLARRVVRVPNACDFDAFA
                     AIPAPAAKPAPVIGYYGAIADWFDSDLVADLAERRPDWRFLLVGSTYTADVSRLSTLP
                     NVELRGEVSYGDLPRWIERMDVLLIPFRRTPLTEATNPVKAYEILAAGRPLVSVPLPE
                     VAALGDLVRLAGTPEEMEREVLAALASDTPGERERRREFARGQTWAHRVEALVPALEE
                     AFPRVSVVVVTWNNREWNARCLASLREKSEWPNLDVIVVDNASTDGTPDLLGEEAARD
                     ARIRVVLNDTNAGFAVANNQGLELATGDVLVLLNNDTMMTRGALASLVRHLRADRSIG
                     LLGPSTNEIANEGRVAVGYSTLDEVPAWAAEFVREHDGELVETSMLAMFCLAMTRETW
                     SRTGPLDERFEVGMFEDDDYSRRVRELGLRTVCARDSFVHHAGRASFGALGDARYLDI
                     FNRNKARYELKWGLWLPHVAPEAMKMVPAFRRELAYRMEQAGADPSRVVVILPTIGWN
                     IALIQRPHHLARALARAGYFVVFDCSNSAAESFGGFQEVEKNLLLFRGPVEILTELAS
                     PILWTLPYNAGHVDSWPTRRIVYDCIDDLAVFPYNQELLRQHHERMLREADVVACVSR
                     PLLQECRRVREDALYLPNAVDVDHFAAPAAAHEPPAALRPVLDAGRPILGYYGALASW
                     LDVELLESAARLRPDWSFVLVGIRLADAPSLEALERLPNVYVLPPQPYRDIPAVLACF
                     DVAFIPFVVNEITHATSPLKLFEYFAGGKPVISSPMPECAAFPEVRIARNAGELAGAL
                     DAALLDSRDPGHQTRVRRVATENSWKARVRAAIASLAPGATRPWPGEPAPGRLQGVER
                     FSYQGQHREGRCCVCGETTRFTHGDPAAFRESLVCRVCGATSRYRSIARGLLRAIAEL
                     GGPAAESLEELPKGHAGPRLRIFDTQQPFAAPSCAYPIPDLLAACGWIDVEIGLYRPT
                     DPPGTRYGPRLTNQDLQALAFPDASFDIVVTSDVMEHVRLDEAAHREIHRVLRDGGVY
                     LFTVPHSRAWPRTLVRRRVVDPADPSRDVDDLPPEYHGDTNSAEGGALAYRAYGTDLD
                     SLLEKLGFSVDYTKADVPELCIHATELFYCGKVPATSPRSPARR"
     gene            9799..10032
                     /locus_tag="PLP50_10400"
     CDS             9799..10032
                     /locus_tag="PLP50_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA51999.1"
                     /translation="MRGLPVLLALVLAPVLVATPALAYVDPAASSLILQVVLGGIAGL
                     GVLLRLFWGRIRRWVRGESQPAADGKSPAGSSR"
     gene            10029..11444
                     /locus_tag="PLP50_10405"
     CDS             10029..11444
                     /locus_tag="PLP50_10405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA52000.1"
                     /translation="MNPDPGSFRDRSGRVYRDGDRVFRGLSSAALEAWQQLERCGLYA
                     NLREGERIVGTRLVPSPEVPPALAALGWSAVLEHDAVPFVSHPWEWPFEMLRDAALLE
                     LDVLDEALAKGMVLRDGTAHNVQFLGARPVFIDVPSLGPYRAGETWAGYRQFCSEFLN
                     PLLLESARSLPAHVLLRGALGGVPPGVCAGALSTRDLFRRGVLTHVWLHAKADSRYRG
                     TGHNVRSELALAGFSAELVKRNSRSLRRAIEALPEPRQESAWEGYETNTSYTADDTRL
                     KEEVVRRATAARAARLVWDLGANTGHYSRIAAEAAPCVVAIDSDAGCVGRLYASLRNT
                     RERRILPLLGDVTDPSPGLGWRLGERGPLESRGRPDVVLALALVHHVVLHGNVRTEEL
                     ADWLMGLGRTLVVEFVTKDDPMAKRLLLNRDDQFEDYDLKAFEAALERRGRIVERHLL
                     CEGTRVLLVCEVSVPTSHTAA"
     gene            complement(11560..13746)
                     /locus_tag="PLP50_10410"
     CDS             complement(11560..13746)
                     /locus_tag="PLP50_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPA52001.1"
                     /translation="MTRWPTLALVASCVPFSVLVFSPIGVWRHNVLEFPFRISEALPY
                     LLGLAAAGAAVSFSVLLLFRGPWRGRAATLLLGLAAALWIQGTFLTWSYGALDGRAID
                     WAQKPWRGWLDLVVWAIVLGLFLFGWRRVRPHAALIAGALLAIPGVAALVSVLSVDAY
                     PSYRRSEVDETLLFRLSSSRNVVVLVLDGFQTTELQRLLSDEPALRERFSGFTYFRNA
                     LADFPITHASLPAILTGRLCDNSEPYLDFIESAYSSESSLPKVLKQRGYTVDLYGMFR
                     AVWADESVMSNVRPPRRGLRGFGGDLAYLLDLGLFRALPQAGKKLVYANQAWSIRRLA
                     PRLGLGQRAAVSIAGGIRDNLDFLDEFRRSTTPQEPGPVFKLFHLLGPHPPLSLDEKG
                     ERASLPYDPTNYRRASVGCVRVAEAFLRELRRRGLYDGSLLFILSDHGYGVPFELPSD
                     VAVRGARAPAPFGRALPLVLVKPIGSTGPMRTSDAPVLLSDVPRTAMALLGLPPGSTP
                     GSSMFDLRDDTVRSRVHTAVVDTNWEKSRYLSVMTDYSVDGFSWSKSSWRPTGRSRGS
                     YADALVAFRALERNPFLGRPVSYSSAGSDARMLVTNEGTLLIYFPQADPGRGDHAGVE
                     FPLEGLEKGRSYRLEVEVVDPCAARAPGRFRQDLWLGGRLIDTYDLAQDAGTGSRRVT
                     FEFRALARNATLKAEVRAVGKPEKGWEWGSSAGIGIRGIQLQATGP"
     gene            complement(13799..16186)
                     /locus_tag="PLP50_10415"
     CDS             complement(13799..16186)
                     /locus_tag="PLP50_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPA52002.1"
                     /translation="MTSPLVPLLGLLCILGLAVPALLLGRAVLGRLRFDGGAEVIALS
                     MALGLGLLSHLLLLLGLLRLLRTPVTVALLVIVSAGSIAWLRRIGVRPLGGWMPRGEH
                     GSGGGSPRPRRTFAFLLVAVVGVALAPYLLISLYPPTAFDATMYHLAAAKEFAQQGAV
                     VVVPNLRFPVYTFGVHVLYTALLELGSDLAPALLSTLASALVALLLVAWGRAAEGWRT
                     GLAAGLLWLASPIVLYVSTIPYLDTMLTLFVTSAGYCFWRWHRDDCRLSLALSGAFVG
                     LACGVKYTGLFELATLGILLLVLAARRPKALAAIATFGVSALAAGAPWYIRSAWLTGN
                     PTWPFLASLFGAGPYWTSRDLADAWADLRTPGMGHDVKALLALPWNLFAHGTRFYAST
                     ANELSRAIPLALPLVLVGGATLTSGWVVLVLLSSLVLSWFLGSQVLRYLLPALPIYCL
                     LAAYGFTTLLKRIALRRRPPAATAALVVLAVLLPLRVARVGWGQLRSSGPPPLTDADR
                     ERYLSSSYPAYRALLAAPQDGGAVYSLFGSSLAYYSPARFAGDWFGPWPYRTVLAAAR
                     SGGRDLHALLTTWGYEYLHVARNRMFSQANAAVPEDEEFRRLFQVVYADEHAELYRLL
                     QEEVPPSPEVQLLRNSGFERLDDADRGAAWGTFGSPRFDSTGAESRSGKAAVLVDQRN
                     TIYQPVAVEPNGAYRLRMFARAASGPGAVVRLQLNWTDQHGKPAGLYLRPVKLADGWV
                     ELTAVCRAPGTALRATVYGSCHEGCGAGVWIDDLTLSEIQEVPLVSSPWPKGERP"
     gene            complement(16273..16524)
                     /locus_tag="PLP50_10420"
     CDS             complement(16273..16524)
                     /locus_tag="PLP50_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52003.1"
                     /translation="MCDGTGLGVTRLSWSAPAGGAVEVRVGKPDGTLFARTGSVGEKD
                     TGKWVGEGTVFYLVRAAIAGEPTSVLGSTKVHVTTQGCK"
     gene            16930..18747
                     /locus_tag="PLP50_10425"
     CDS             16930..18747
                     /locus_tag="PLP50_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52004.1"
                     /translation="MTGAPLRSKGAEAALVIGIGALGVALRLAWIRHFPTLPVSDFAS
                     VLEFAEALARDLAATDARQWYSLSPGASLLISLGLRVGVASPADTARVMTAVVTGLIP
                     LLPVLGWRGIAPYWVRATTGLLLAVWPGQVFFSGVIAQDNWAILPTVALGVLGCRTLV
                     DGKARPVASAALLGLAFLTRQEMLAALAPLALAGAGLVPRRPGRGRLAAGFGGTLAAV
                     LLLVAAHRYAGSGRVALTTTHGSLAMLCSTIPGASWAYNASPYSYLETFAPGQEKSEV
                     AFRLVKQELRRRPLYHSVRAFYFTFAPVFRSSRESMYWSLGAHVLPDAERDRGARVLA
                     ALRTPLTAEMLVVVWAGFAVLLVALASRNTSLLLLCLAVVLKVGVHALTVNQPRYIYP
                     VTALILLAAPLAAWVGRRERGGRRLALAAAAAVLCTFALAFLTRIAERWTRAHDTPIE
                     YRFALWDGSGGERLRCRTANAGIWSWYPKRDEKVVSFFRPDVAPGDVAEVACEVPAGS
                     YVPPAALEIAVAPEVGRGLFELSLGRDGQDPAVVDSRLLADGRPIELPLVPGELSRIT
                     ARLKAEDRAFGRGLGHAATMALRLREPTPVPPPGATRVR"
     gene            18875..>20411
                     /locus_tag="PLP50_10430"
     CDS             18875..>20411
                     /locus_tag="PLP50_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HPA52005.1"
                     /translation="MNRTRLAGFLVSTTLLLSGFARADGPALVRVDRAGPGDRDALVA
                     AGVPLVLEQERLFLALGDAPAIRQRLQSLGRTGVVVDPDTTGSSYYVAGLRPGATAGD
                     LAATCGELLVVEDGWALVRTPGELGDPCVDSTKWFLRRVPMEPLRPTAPLPEPYASWQ
                     RGEPLALDPHPVVQTIVDDLTPATIQANWEAIVNVATTRYSRSAGCVTATDSVFAAFQ
                     TLGLNPVRQNHTSGHAPNVIGTITGYAHPEQVIIVIGHIDDVPSSGAAPGADDNASGA
                     ATVVSAAQALAGYTFEKTVKLIAVTGEEQGLYGSAHYADEAWATGEQIIAVLNADMTG
                     WAGDGLPASGENLDVNTNAASAWLGTLLAEAATAYGTGCVVNSFSCSSMVYSDHAPFW
                     DYGWSAICAITDNEGSCGQGGSYPYYHTVNDTLANCGNPSFYASTVKAFVAAAAHLAV
                     PLCGGGAFPPVPTGVAAAATGVNQITVSWTSGGAGLEYEVLRGRGGCEAGDFTPVATT
                     TSSH"
BASE COUNT         2956 a         7233 c         7284 g         2938 t
ORIGIN      
        1 agggcgctcg ggctcccgat gtacacccac cccgtagctc cgtaatcgcc aaccggccgg
       61 ggggctccgc cccccggcca tcgacctgac cgaagggccg ggcgcgaaag cgcccggccc
      121 ttttcgtgtg tgcgacgggc aaggcgcgaa gcgccgtgac cggcgctgtc gaccgagagg
      181 cgaaaccgga ccggaacgtg acctggaggt ggaggttctc cgggaatcga gatggcctgg
      241 cccccgagcc gagcggagac ggccgcgtcg cgtgcgatgg cgggaaggag accgcagcag
      301 cgttccggcc cgaagagcac cacgcgcatg gaggttgggt ttgccacact ccgagcctcg
      361 tcgcgaacgc aagttgagcc gacctccgcg cctgcctata ctgccgcggt gacggtcccc
      421 tcccggcccc acgatcttca gggtggccgc cgtcgcgcaa gtcccaaagt gagcgctccc
      481 cggtcccgcg tggtcgtctg cggctactac ggattcggga acgccggcga cgagctgatc
      541 ctggccgcga tgctccgcga gctccgcacc cagcggcctg gcctcgtcgt gaccgtgatc
      601 tccggcgacc ccgagtcgac ccgcgcgagc cacggtgtcg acgcggtcca ctggcaggac
      661 gtgcccgcga tcggagcacg ggtccgccac gcggacctcg tcgtcgtcgg cggagggggt
      721 ctcttccagg agtactcggg catcgacccc gacagcctct tcaccgaccg gcactacgcc
      781 atcacgttct acgccgagtc cgcgatcctg ggcgcgctct gcggccggcc cgtcatgctc
      841 tacgcggtcg gggtcgggcc gctcttctcc gagcacgggc ggcgcgtggt gcgcgccgcg
      901 tgcgacgcgg cgcaggtcat cacgctgcgg gacgaggagt ccgcgcgcgt ggtcgcctcg
      961 ctcggagtcg atccggagcg cctgcgcgtg accgcggacc ccgtcttcgc gctgccggcc
     1021 ggcggcgacg cgcggaggcc ggcagatgcc gcccgagcga tcccgcccgg ggaggggccg
     1081 ctcgtgggcg tggcgctgcg ccactgggac gtgggggtgg ctccgtactt ctgggagagg
     1141 gaggtcgcgg caggactgga cgcgttcctg gagagccacg aggcccgcct gctcttcgtg
     1201 ccgttccagc atctcgcccg cgcgaacgag aacgacgtcg cggtcgcgga acgggtgcgc
     1261 tcccggcttc ggaagcccga gcgcgcgtcc gtgtttccgg gcggcggaga tccgagcgcc
     1321 ctcggagccg tcctcgggtg ctgcgacctc gttctcggaa tgcgcctcca cgcgatgatc
     1381 ctcgccgcga cggccggcgt ccctgccgtc gctctctcgt acgacccgaa ggtcgagctg
     1441 gcgatgcggc agctgggcgc ggaacggttc gtcgttccgc tcgcggacgt cgaggcccgt
     1501 acggtcgcaa cactcatgga ggaggccctg gccgagggcg cctcccgccg cggggacctc
     1561 ggcgaacgcg tcggtcggct ggcggagcag gcgcggagca acgccgtcgc ggcgatcgac
     1621 ctgctcgatc gaggagcccg ggagcggacg ctgagcccgg ccctcgccga ggtgatcgcc
     1681 cggtcggtga cgtcgcgatt cgaggtcgag gcgacgctgc ggggcgaagc ggcggccctt
     1741 cgcgaagcgg cgggactggc cgaggccgcc ctggcggagt caggcagcgc gcgcgcagcg
     1801 gcggaagcgg cggccgagtc gcgagcggga gagctacgcc ggaagggcga agagctcgcg
     1861 gcctgcgaag ccgagctggc gcaggcaagg agcgtgatcg gacgccggga gacggagctg
     1921 aaagaggtcc acacccgcct gatgtcgctc gacacggagc tgcgacggag tcggactgat
     1981 ctcgcggcga ggtctgcgga gctggaggag gtccgcggcg acctctcgac ggagtcggcg
     2041 gagctggagc cggcgctgga cgagctttcg cgtcaggacg aggcgctcga gggttcccgg
     2101 acggaggtca gggggcttcg cgaccgcgtg gccggcctcg agggagacct ggcccgcatc
     2161 caccggtcac ggctgtggaa gctggccacg cgctactggc ggttcctcga ggcgatcggc
     2221 cgcctgccgg ggccgaggag ctagccggga gcccgggccg cacccggcca gcctgctaga
     2281 gtcgatgctc gtcattcggc gccgcgagag cgggaatcga gggggatcga ccatcaggca
     2341 cactgctttc gtcgcagacg ggccgcggcg ggccacggag agcgcccagc cgcggcgagg
     2401 agggctcccc ggatgagcgg acccggagcg gatccgcgcg ccggcgtcct ggtcgtcatc
     2461 ggcactcgtc cggaggcgat caagctcgcg cccgtggtcg aggcgctgca aggctcggag
     2521 acgctccgcc cggtgatcct cgccacgtct cagcaccggg agatgctgcg ccaggcgctc
     2581 gcccctttcg acctcgaacc cgacgtcgat ctcgaggtga tgaccgccgg tcagtcgccc
     2641 accatggtcg cggcccgcgt gctggaccgg atcgagcggt cgctccagga catcgcgccg
     2701 gcgttcgtcg tcgtacaggg cgacacgacc accgccctgg ccgcggcctg ggcgtccttc
     2761 tacgccggga ttccagtcgc ccacgtggag gcgggtctcc ggagcggcag gatcgactcg
     2821 ccgtttccgg aggagttcaa ccggcgcggc gtctcggtcg cggcctcgct ccacttcgct
     2881 ccgaccccgg gggcgcgcga aaacctcctc ggggagggcg tctcgccgga cggcgtcttc
     2941 gtcgtcggga atacgaacgt cgatgccgtc gcgcggatcc tgaagggccg tcggcgtccg
     3001 gagccggagc ccggtcgcgc gacgatcctc gtgacgcttc atcgccgcga gagcttcggc
     3061 ccgccgctcg acagggcgct cggcgccatc cgccgcctcg cgctcgagca gggcgatcgc
     3121 gtccgggtcg tctaccccgt tcacccgaac ccgaaggtct cggagcccgc gcgccggcat
     3181 ctcctggacc tgccgaacgt cgagctgcgg ccacccctcg actaccccga gttcctcgag
     3241 gtcttctcga cctcgcggtt cgcgctctcg gactcgggcg gtgtgcagga ggaggctccg
     3301 acggtgaggg tcccggtcct catcgcccgt gagacgaccg agcgcccgga agtcctcgag
     3361 tcggggtggg gaatcctggt cggcaccgac ccggaccgga tactgggcga gtcgcgtcgg
     3421 cttctcgacg acgaggacgc ctatcgggcc cggtgcgccg gtccgaatcc cttcggcgac
     3481 ggaaccgccg cgcgccggat cgtggacgtg ctctcgaccc gatgggcggc gaggcctcgt
     3541 tccccgctcg cgacgccgga gctccggacc tctcgaggag cgaagtgaga tgagcgcgga
     3601 agaccgggtc gcttcgctgg cgcggacgaa agccgggctc gaggccgacc ttctggagac
     3661 gtcgctcgac ctgcgccgcg cctcggagga ttacgaggcg ctcgcggcgg cgttccgcgc
     3721 ctcggccgaa cgcctccgcg aggcggccgg cgcggtctcc gccgcgcacc acgagcgcga
     3781 ggagctcgtc cgcgtcgtcg cgtctctcca ggccgagctc gcggcgacgc ggcgggaccg
     3841 ggacgaggtg aagcgaagcc tggaggaggt ctgctcgacg cgcgtgtggc ggctcgggac
     3901 gctctactgg cggctcctcg gtcggctcgg actcctcccg gcgaaggcgg gaggagcgct
     3961 cgccggcgcg gcgagcgtcg ccagcgaggc ggccggaccc gtcgcggacg gtccgggtca
     4021 ggcgatgctc gcggtgccgg cggcgcctcc tggcgtcgag atgcccacac ggaccggcag
     4081 gtcccgcagg gaccctgcgg cgccgggact gcctgacgtc gtctgcttct cgatcgtgga
     4141 atgggagttc ctgttccagc gtccccagca gctcctctcc cggctcgccg acctcgggca
     4201 ccgcgtctac tacatcagcc agttcttcga gcccgacccg ggagctcccg aggtccaccg
     4261 cctccgttcg cgcgtctaca cgctccggct gcgcggcagc tccgggaggc tcttctccga
     4321 ggagctgacg ccgaccgacg ccggggcggt cttcgagtcg ctggcatcgc tccgcgagcg
     4381 cgaggggatc acggccgccg tgtcggtcgt ccaccagccg ttctggtggc cgctcgccga
     4441 gagggcgagg agcgcgttcg gctgggcggt gctctacgac tgcatggacg atcacggggg
     4501 cttccagacg aacgcccggc cggtgggagg cgcggaacgg gggatcctcg aaggcgccga
     4561 caccgtcgtg gccagctcgc gggtcctcga gagccggatc cacgccgtga aacgtccggt
     4621 ggagctgatc ccgaacggct gcgactacga gtacttctcg tcgatggctc cgcgttctcg
     4681 gggcgtgcgg ccgacggtcg gctactacgg ctgcatcgcg gactggttcg aggcggacct
     4741 cgtggccgac gtcgccgaga gacggcccga ctgggagttc ctcctgatcg gcccgaccta
     4801 cctcgccgac ctgacgcgcc tgccggcgct gccgaacgtc tccttccccg gagtcgtgcc
     4861 gtacggccgg ctcctcgacc ggatcgagcc gatcgacgtc ttcctccttc cgttccggcg
     4921 gtcgacgctc acggaagccg ccaaccccgt caaggcgtac gagatcatgg cgacggggcg
     4981 ccccctcgtc tcggttcctc ttccggagct cgagccgttc ggggacctcg ttcgcttcgg
     5041 gaacgggccc gtcgagttcg agcgacggat cgaagaggcg ctccgggagg acgggcccga
     5101 gatcgtggat cggcgacgcg acttcgcccg gaggaactcc tgggacacac gggcagcgcg
     5161 gttcgcggaa cttgtgcgcg cgcttggagc cggccgggag gccggtccgc cgcgcccgga
     5221 ggacgacgct tgagctcgag cggagacgag cggcccccgc agggtcgcga cgacgggctc
     5281 gcggaggtcg aggagctgag agccctcgtc gaccgcagcc gaaatcgcct gcggaccctc
     5341 cgccagcggc tccgtcggcg cgagctgcag gtggccgcgc ggagcggaag cgtcgaggag
     5401 ctcctgcgcc gcgcggagga gcgcgggcgt gggcggatgg cggcgcggca ggagagcgcg
     5461 tccccgcacg gcacgcggcc cgccgggccc gacgcgtcca ttcaggccct gaccgcggag
     5521 atcgcctcgc aggcggagac catcgccgcc ctcacgcgag agcgtgatgc cctcggagcc
     5581 gagctccggg gtatccacag ctcgaagctc tggcggctgg gggccgcgta ctggagcgtc
     5641 cttcgcgcgg tcggcctcgt gaagccgccg cctgggccgg agcccggcgt gaccgctctc
     5701 ccggcgacgg aaggtacggc ccccctcgga gttccggagc cggccgccgt gccgggtccc
     5761 gccgtcgggg ccgtcgtcga caccgccgcc gagaccgccc ccgtcgtcgg ctcccgaggc
     5821 gaaggcgacg gccatgcccg gcccggcggc gcggcattcg acgtcgtctg cttctcgatc
     5881 atcgaatggg acttccggtt ccagcgtccg cagcagctga cgagccggct cgcggatcgc
     5941 ggacaccgcg tcttctacgt cagccaggtc ttccggcccg agggaccggc cgtgacactt
     6001 cgggagattc ggccgaacgt ctgggaggtc tcgctgcggg ggccggcgaa gaatgtctac
     6061 cgggacgacc tcgcgccgtc cgaggtcgag gcgctcttcg agtccctcga cgtcctccgg
     6121 cgcgaggaag ggctcggggc gacggtctcg ctcgtccagc tgccgttctg ggcgcccctc
     6181 gtcgaggagg cgcgttcgcg cctctcctgg ccggtcgtct acgactgcat ggacttccac
     6241 gcgggcttct cgacgaacga gagcgcgatg ctctcgcggg agcggcagct cctctcgggg
     6301 gcggacctcg tcgtcgtctc ctcccgcttc ctccaggacg aggccacgcc gctcgcccgc
     6361 cgcgtcgtga gggtcccgaa cgcctgcgat ttcgacgcct tcgccgcgat tcccgcgccg
     6421 gcggcgaagc cggcgcccgt gatcggctac tacggcgcca tcgcggactg gttcgactcc
     6481 gacctcgtcg cggacctggc ggaacggaga cccgactggc gcttcctcct cgtcggctcg
     6541 acctacacgg ccgacgtgtc gcggctctcc acgcttccga acgtcgagct gcggggcgag
     6601 gtctcgtacg gggacctccc ccgctggatc gagaggatgg acgtcctcct gatcccgttc
     6661 cgccgaaccc ccctgaccga ggcgacgaac ccggtgaagg cgtacgagat cctggcggcg
     6721 gggcggccgc tcgtctcggt gccgctcccg gaggtcgcgg ctctcggcga tctcgtgcgc
     6781 ctcgccggga cgccggagga gatggagcgc gaggtcctcg cggccctcgc ctcggacacc
     6841 cccggggagc gcgagcgcag gcgcgagttc gcccgcgggc agacctgggc gcaccgcgtc
     6901 gaggcgctcg ttccggctct cgaggaagcc ttcccgaggg tctcggtcgt cgtcgtcacc
     6961 tggaacaacc gcgagtggaa cgcacggtgc ctcgcgagcc tccgggagaa gagcgaatgg
     7021 ccgaacctcg acgtgatcgt cgtcgacaac gcctcgaccg acggcacccc ggacctgctc
     7081 ggggaggagg cggctcgcga cgcgcggatc cgcgtcgtcc tgaacgacac gaacgccggc
     7141 ttcgcggtcg cgaacaacca gggcctcgag ctcgcgaccg gggacgtcct cgtcctcctc
     7201 aacaacgaca cgatgatgac gcggggggcg ctcgcctccc tggtccgcca cctgcgtgcc
     7261 gatcgatcga tcggcctcct cggaccctcc acgaacgaga tcgcgaacga aggaagggtc
     7321 gcggtcggct actcgacgct ggacgaggtc ccggcctggg ccgcggagtt cgtccgggag
     7381 cacgacggcg agctcgtgga gacgtcgatg ctcgcgatgt tctgcctcgc gatgacgcgc
     7441 gagacgtgga gccggacggg gcctctcgac gagcgattcg aggtcgggat gttcgaggac
     7501 gacgactact cgcggcgcgt gagggagctc gggctccgca cggtctgcgc ccgggacagc
     7561 ttcgtccacc acgccggacg ggcgtcgttc ggcgccctcg gcgacgcccg ctacctcgac
     7621 atcttcaaca ggaacaaggc tcgctacgag ctgaagtggg ggctctggct cccgcacgtc
     7681 gcgcccgagg cgatgaagat ggtcccggcc ttccggcgcg agctcgcgta ccggatggag
     7741 caggcgggcg ccgacccgtc gcgcgtcgtc gtcatcctcc ccacgatcgg ctggaacatc
     7801 gcgctcatcc agcggccgca ccacctcgcg cgggcgctgg cccgggcggg gtacttcgtc
     7861 gtcttcgact gctcgaacag cgcggccgag agcttcgggg gcttccagga ggtggagaag
     7921 aacctcctcc tgttccgggg acccgtggag atcctcacgg agctggcctc cccgatcctc
     7981 tggacgctgc cgtacaacgc cgggcacgtc gacagctggc cgacgcggcg gatcgtctac
     8041 gactgcatcg acgacctggc cgtcttcccc tacaaccagg agctgctccg gcaacatcac
     8101 gagcggatgc tccgcgaggc ggacgtcgtc gcctgcgtct ctcggccgct ccttcaggag
     8161 tgccggcgcg tgcgggagga cgccctctac ctcccgaacg ccgtcgacgt cgaccacttc
     8221 gcggcgccgg ccgccgccca cgagcctcct gccgcgctgc gcccggttct cgacgcgggg
     8281 aggccgatcc tcggctacta cggggccctc gcctcgtggc tggacgtgga gctgctcgag
     8341 tcggccgcgc gcctccggcc ggactggagc ttcgtcctcg tcgggatccg gctcgccgac
     8401 gcgccgtcgc tcgaggcgct cgagaggctc ccgaacgtct acgtcctccc gccgcagccc
     8461 taccgggaca tcccggcggt cctcgcgtgc ttcgacgtcg ccttcatccc gttcgtcgtg
     8521 aacgagatca cccatgcgac ctcgccgttg aagctcttcg agtacttcgc ggggggcaag
     8581 cccgtcatct cgtcgccgat gccggagtgc gctgccttcc ccgaggtgag gatcgcccgg
     8641 aacgccgggg agctcgcggg cgccctggac gccgctctcc tcgactcccg cgacccgggc
     8701 caccaaaccc gcgtccggcg ggtcgcgacc gagaactcct ggaaggcgcg tgtccgcgcg
     8761 gcgatcgcct cgctcgcccc cggagcgacg cggccgtggc ccggagagcc ggccccgggt
     8821 cgtctccagg gcgtggagag gttctcgtac caggggcagc atcgcgaggg gcgctgctgc
     8881 gtctgcggcg agacgacccg gttcacccac ggcgatccgg ccgcgttccg cgagtcgctc
     8941 gtctgtcgcg tttgcggggc gacgagccgg taccgctcga tcgcccgcgg cctccttcgg
     9001 gccatcgcgg agctcggcgg accggccgcg gagagcctcg aagagctccc gaaaggccac
     9061 gccggcccga ggctccgcat cttcgacacg cagcagccgt tcgccgcgcc gtcgtgcgcc
     9121 tatccgattc ccgaccttct ggccgcctgt ggctggatcg acgtcgagat cggcctctac
     9181 cgtccgaccg acccgccggg cacccgatac gggccccgcc tcacgaacca ggacctgcaa
     9241 gccctcgcgt ttccggacgc ctccttcgac atcgtcgtga cgagcgacgt gatggagcac
     9301 gtccgcctcg acgaggccgc gcaccgggag atccatcgag tcctccggga cggcggcgtc
     9361 tacctcttca cggtgcccca ttcgagggcc tggccccgca ctctcgtgcg gcgccgcgtc
     9421 gtggacccgg ccgatccttc ccgcgacgtc gacgacctgc cgccggagta ccacggggac
     9481 acgaactccg cggaaggggg cgcgctcgcc tatcgcgcct acggaacgga cctcgactcg
     9541 ctcctcgaga agctcggctt ctcggtcgac tacaccaaag cggacgtccc ggagctctgc
     9601 atccacgcga ccgagctctt ctactgcggg aaggtcccgg caacgtctcc gcggagcccg
     9661 gcgaggcgat gatggtgcct ctgcccgtcc ttcccgcgtc gcgatcgtcg aatcgccttt
     9721 gaaccagggg gtcagctcat gccgttcctt ccccgtcgtc gtccgatccc gacggccgtc
     9781 tccaggcctc gagtgcccgt gcgaggactg ccggtccttc tcgctctcgt cctcgcgccg
     9841 gttctcgtcg cgaccccggc gctggcctac gtcgatccgg cggcgtcgag tctgattctc
     9901 caggtcgtcc tcggaggtat cgccggtctc ggagtcctcc tgcggctgtt ctggggcagg
     9961 atccgtcgct gggtgcgtgg ggagtcccag ccggccgccg acgggaagtc gccggcgggc
    10021 tcgtcacgat gaaccccgat cccgggtcgt tccgggatcg ctcgggacgg gtctatcgcg
    10081 acggcgacag ggtctttcgc gggctttcca gcgccgcgct cgaggcgtgg cagcagctcg
    10141 agaggtgcgg gctctacgcg aacctgcgtg aaggcgagcg aatcgtcggc acgcggctcg
    10201 tgccttcgcc ggaggtccct cccgcgctcg ctgcgctcgg gtggagcgcg gtcctcgagc
    10261 acgacgccgt cccgttcgtc tcgcacccct gggaatggcc gttcgagatg ctgcgcgatg
    10321 ccgcgctcct cgagctcgac gttctcgacg aggcgctcgc gaagggaatg gtcctgcgcg
    10381 acggcacggc ccacaacgtg cagttcctgg gtgcgcggcc cgtcttcatc gacgtcccgt
    10441 cgctcggacc ctaccgggcg ggagagacct gggcgggcta ccggcagttc tgctcggagt
    10501 tcctgaaccc cctccttctc gagagtgcgc ggtccctccc ggcgcacgtc ctccttcgcg
    10561 gagccctggg tggcgtcccg cctggagtct gcgcgggagc gctgtcgaca cgcgacctct
    10621 tccgtcgcgg ggtcctcacg cacgtctggc ttcacgcgaa ggccgattcc cgctaccgcg
    10681 gcaccggaca taacgtccgg tcggagctcg cattggcggg attctcggcg gagctggtga
    10741 agcggaacag ccgctcgctc cggcgggcga tcgaggcgct cccggagccc cgccaggagt
    10801 cagcgtggga gggctacgag acgaatacct cgtacacggc ggacgacacc cgactcaagg
    10861 aggaggtcgt ccgaagggcg accgccgcgc gggccgcgcg actcgtctgg gacctcggcg
    10921 caaacacggg gcactactcg aggatcgcgg cggaggcggc cccttgcgtg gtcgcgatcg
    10981 attccgacgc cggctgcgtc gggcgcctct acgcgagcct gcggaacacg agggagcgcc
    11041 gcatcctccc gctgctcggc gacgtcaccg acccttcacc cgggctcggc tggcggctcg
    11101 gcgagcgtgg cccgctcgaa tcccgaggtc gacccgacgt cgtcctcgcc ctggcgctcg
    11161 ttcatcacgt cgtgctccac ggcaacgtga ggaccgagga gctcgccgac tggctcatgg
    11221 gcctcgggcg gactctcgtc gtcgagttcg tcacgaaaga cgacccgatg gcgaagaggc
    11281 tcctcctcaa ccgcgacgac cagttcgagg actacgacct caaggccttc gaggcggcgc
    11341 tcgagcgccg cggccgcatc gttgagcggc acctcctttg tgaggggacg cgcgtcctcc
    11401 tcgtctgcga ggtctcggtg cccacatcgc acaccgctgc ctgagaccgt tcttcctgac
    11461 gaggttcgct cccgcggcgg cttccgcacg cgactggcgt tcccggggcc ggcacgccgt
    11521 cgatccggtc accacggccg gccgggctcg ccaacgcggt cacgggccgg tcgcttgcag
    11581 ctggatacca cgaatgccga ttcccgccga gcttccccac tcccagccct tctccggctt
    11641 ccctaccgcc cgtacctcgg ccttcagcgt ggcattccgc gcgagcgccc gaaactcgaa
    11701 cgtcacgcgc cgggagccgg tgccggcgtc ctgcgcgaga tcgtaggtgt cgatcaatcg
    11761 gccgcccagc cacaggtctt gacggaaccg gcccggcgcc ctggcggcgc acgggtcgac
    11821 gacttcgacc tcgagccgat acgaacgccc cttctcgagg ccctcgaggg ggaactcgac
    11881 tcccgcgtgg tcgccccgcc ccggatcggc ctgggggaag tagatgagga gcgtcccctc
    11941 gttcgtcacg agcattcgcg cgtccgatcc agcgctcgag tacgagaccg gacggccgag
    12001 gaacggattc cgttcgagcg cacggaaggc gacgagcgcg tccgcgtagc tgccgcgcga
    12061 tcgtccggtc ggccgccagg agctcttcga ccaggagaac ccgtcgacgg agtagtcggt
    12121 catgacggag aggtaccggc tcttctccca gttcgtgtcg acgaccgcgg tgtggacccg
    12181 acttctgacc gtgtcatctc gcaggtcgaa catcgaggag ccgggtgtcg acccgggagg
    12241 aagccccagc aacgccattg cggttctcgg cacgtcgctg agaaggacgg gcgcgtccga
    12301 ggtccgcatc ggcccggtgc ttccgatcgg cttgacgagg acgagtggca gcgctctccc
    12361 gaagggcgca ggcgctcggg caccgcgaac ggcgacgtcc gacggcagct cgaaggggac
    12421 gccgtagccg tggtccgaga ggatgaagag aagggagccg tcgtaaagcc cgcggcggcg
    12481 cagctcccgg aggaacgcct cggcgacccg gacgcagccg acggaggccc tccggtagtt
    12541 cgtcgggtcg tagggaaggc tcgccctctc acccttctcg tcgagggaga ggggcgggtg
    12601 agggccgagc aggtggaaga gcttgaagac cgggccgggc tcctgcgggg tcgtagagcg
    12661 gcgaaactcg tcgaggaagt cgaggttgtc ccggatcccg ccggcgatcg acacggcggc
    12721 cctctgtccg aggcccagtc gcggggccag tcgccggatg ctccacgcct ggtttgcgta
    12781 gaccagcttc ttccccgcct ggggcagagc ccggaagagc ccgaggtcga gcaggtaggc
    12841 gaggtcgccg ccgaagccac gcaggccgcg ccgcgggggc cgaacgttgg acatcacact
    12901 ttcgtcagcc catacggccc ggaacatgcc gtagagatcg acggtgtacc cgcgctgctt
    12961 gaggaccttt ggcagggacg actccgagct gtaggcgctc tcgatgaagt cgaggtaggg
    13021 ctcggagttg tcgcagaggc ggccggtcaa gatcgcagga agcgacgcgt gcgtgatcgg
    13081 gaagtcggcc agcgcgttac ggaagtacgt gaagccactg aagcgctctc gaagggccgg
    13141 ctcgtcggac agaagacgct ggagctcggt cgtctggaag ccgtcgagaa cgagaacgac
    13201 gacgttccgg gaggaggaga gccgaaagag cagtgtctcg tcgacctcgc tgcgccggta
    13261 cgaagggtag gcgtcgacgg aaaggacgga cacgagcgcg gcgacgcccg gtatcgcgag
    13321 cagcgcgccc gcgatgagag ctgcgtgcgg gcggacgcgt cgccagccga agaggaagag
    13381 cccgagcacg atggcccaga cgaccagatc cagccagccg cgccacggct tctgcgccca
    13441 gtcgatggct ctcccgtcga gggcgccgta gctccaggtg aggaacgtcc cctggatcca
    13501 gagcgccgcg gcgagtccca gaaggagcgt agccgcgcga ccgcgccagg gaccacggaa
    13561 gaggagtagc accgagaagc tcacggccgc tccggcggcg gccaggccga ggaggtaggg
    13621 cagcgcctcg gagatgcgga acgggaactc gaggacgttg tgtctccaga cgcctattgg
    13681 agaaaagacg agaaccgaga acggcacgca ggatgcgacg agagccagtg tgggccaccg
    13741 ggtcacctcg tcttctcgct gcggatcgct caacgagtct gaatggcgcc tcttcatgtc
    13801 agggccgttc ccccttcggc cacggcgagg acaccagcgg gacctcctgg atctcggaca
    13861 gggtgagatc gtcgatccac acgccggcac cacacccctc gtggcacgac ccgtatacgg
    13921 ttgctcgtaa ggcggtgccc ggagcccgac agacggcggt gagctcgacc catccgtcgg
    13981 cgagcttcac aggccgcagg tacagacccg cgggcttccc atgctggtcg gtccagttca
    14041 gctgcaggcg aacgaccgca ccgggccccg acgcagctct ggcgaacatc cgcaggcggt
    14101 aggctccgtt cggctcgacc gcgacgggct ggtagatggt gtttctttgg tccacgagga
    14161 cggcggcttt ccccgatcgg gactcggccc cggtcgaatc gaaccgcggc gagccgaatg
    14221 tcccccaagc tgctccgcga tccgcgtcat cgagtcgctc gaatccggag ttccgaagga
    14281 gctgcacctc cggcgacgga gggacttctt cctggaggag ccggtagagc tccgcatgct
    14341 cgtccgcgta gacgacctga aagagacgcc ggaactcctc gtcttccgga acggccgcat
    14401 tcgcctgaga gaacatgcgg ttcctcgcca cgtgaagata ctcgtagccc caggtcgtca
    14461 ggagggcgtg gaggtcgcgg cctccgctcc tcgccgctgc cagaacggtg cgatagggcc
    14521 acggcccgaa ccagtccccg gcgaaacggg cgggcgagta gtaggcgagg ctcgatccga
    14581 agagcgagta gacggctcct ccatcctggg gggccgcgag gagggctcgg tacgccgggt
    14641 aactgctcga gagatagcgt tcgcgatccg cgtccgtgag aggcggtggt ccggaactgc
    14701 gaagctgtcc ccaacccaca cgggcgacac gaagcggaag caggacggcg aggacgacga
    14761 gggccgccgt ggccgctgga ggccggcggc ggagagcgat cctcttcagc agggtcgtga
    14821 acccgtacgc tgccaggagg cagtagatcg ggagtgcagg gagaaggtac cggagaacct
    14881 gggagccgag gaaccacgac agcacgagcg acgagagaag aaccaggacg acccagcccg
    14941 aggtgagggt cgccccgccc acgaggacga ggggaagcgc gaggggaatc gcccgggaga
    15001 gctcgttcgc agtcgaggcg tagaacctgg tcccgtgggc gaacaggttc caggggagcg
    15061 ccaggagtgc cttcacgtcg tggcccatcc cgggcgtcct gagatcggcc catgcgtccg
    15121 cgaggtcgcg ggaggtccag taggggccgg cgccgaacag gctcgcgagg aagggccagg
    15181 tcggattccc ggtgagccag gcgcttcgga tgtaccacgg ggcccccgcg gccagcgcgc
    15241 tcaccccgaa ggtcgcgatc gccgcgaggg ccttcgggcg acgtgccgcg aggacgagca
    15301 ggaggatacc gagcgtcgcg agctcgaaaa ggcccgtgta cttcacgccg caggcgagac
    15361 cgacgaacgc gccggagagc gcgaggctca gccggcagtc gtcccgatgc caccgccaga
    15421 agcagtagcc ggccgaggtc acgaacaggg tcagcatcgt gtcgaggtag gggatcgtcg
    15481 agacgtacag gacgatcggg ctcgcgagcc agaggagacc tgccgcgagg cccgtgcgcc
    15541 agccctccgc agcacggccc caggctacga ggaggagcgc gacgagggcc gaggcgagcg
    15601 tcgagagaag cgcgggcgcg agatcgctcc cgagctcgag gagcgccgtg tagaggacgt
    15661 gcactccgaa cgtgtagacg ggaaaccgga ggttcggcac gacgacgacg gcgccctgct
    15721 gcgcgaactc cttcgcagcg gcgaggtgat acatcgtcgc gtcgaaggcc gtcgggggat
    15781 agagcgaaat gaggaggtag ggcgccagcg ccaccccgac gaccgcgacg aggaggaaag
    15841 cgaaggtccg tcgcgggcgg ggagatccgc cgccggagcc gtgctcgccg cgaggcatcc
    15901 acccgcccag agggcggacc ccgatccgcc ggagccaggc gatcgacccg gccgagacga
    15961 tcacgagcag cgcgaccgtc actggggtcc gcaggagccg gagcaaaccg aggagaagca
    16021 ggaggtgaga gagcaggccg agaccgaggg ccatcgagag ggcgatcacc tcggccccgc
    16081 cgtcgaaccg caggcgcccg agaacagcac ggccgaggag aagcgcggga actgcgagcc
    16141 ccaggatgca gaggagcccg agcaggggga cgaggggact cgtcatccga ggtttcgatg
    16201 gctaccaagg gctgcagcgg ctcgcgcgag cgccttcgcg ccgggccgtg cgccaggcgc
    16261 ggaggcgacg cctcacttgc acccttgcgt cgtgacgtgg accttcgtgc tgccgagcac
    16321 gctcgtcggc tcaccggcaa tggccgctcg cacgaggtag aagaccgtgc cctcgccgac
    16381 ccacttgccg gtgtccttct cgccgacgct ccccgtccgt gcgaagagcg tcccgtccgg
    16441 tttgccgacg cggacctcta ctgcgcctcc ggcaggagcg gaccaggaaa ggcgggtcac
    16501 cccgaggccg gtgccgtcgc acacctggat cggattcggg gacgcggtga gggtcccctg
    16561 cgtcgccgga gcgctggacg acgccggcga agagtggccg aaagaggctg cggggtcagc
    16621 ggagggcacc gcttccaccg acggggtctc gctggaatcg ccgcagccgc tcgcggccgc
    16681 gacgagcgtg agaaggacga tcgagacgaa tctggcaggt gatgacctca cggaatgcct
    16741 tccctcctga tttcgtggcg attctctggc ccggagaata cgggaccgat ccgggcgggg
    16801 gaagcgcatc ggcccgacgg tgcggtgccc ccctgtaaca tcgttccccg tgccacccgc
    16861 ccgcgctgca cgtcccgcgt tccggctccc gatccgtccg acggtccgtc gtcgcaggga
    16921 gacgccgcgt tgacgggcgc gccgctccgg tcgaagggcg ccgaggccgc gctcgtcatc
    16981 ggcatcggag cgctcggcgt cgcgcttcgg ctcgcctgga tccggcactt cccgacgctg
    17041 ccggtctcgg acttcgccag cgtgctcgag tttgcggagg cgctcgcccg cgacctcgcg
    17101 gcgacggacg cgcgtcagtg gtactccctc agcccgggag catcgctcct gatctccctc
    17161 ggcctgaggg tcggcgtcgc gtcaccggcc gacacggcgc gcgtcatgac ggccgtcgtg
    17221 acgggactca tcccgttgct gcctgtgctc ggctggcgag gcatcgcccc ttactgggtc
    17281 cgagcgacga cgggcctcct cctcgccgtc tggcccggcc aggtcttctt ctcgggagtg
    17341 atcgcccagg acaactgggc catcctcccg acggtcgccc tcggcgtact cggctgccgg
    17401 acgctggtcg acgggaaggc ccggccggtc gcctccgcgg ccctgctcgg cctcgccttc
    17461 ctcacgcgcc aggagatgct cgcagcgctc gctcctctcg cgcttgccgg ggcggggctc
    17521 gtgcctcgcc ggcccgggag ggggcgcctc gccgcgggct tcggcgggac gctcgcggcc
    17581 gtgctcctcc tcgtcgccgc gcatcgctac gctggctccg gccgcgtcgc gctgacgacg
    17641 acgcacggat ccctcgccat gctctgctcg accatcccgg gggccagctg ggcctacaac
    17701 gcctctccct actcctatct cgagacattc gctccggggc aggagaagag cgaggtggcg
    17761 ttcaggctcg tcaagcagga gcttcggcgg agacccctct accactcggt gcgggccttc
    17821 tacttcacgt tcgcgccggt cttccggtcg agccgcgaga gcatgtactg gagcctcgga
    17881 gcgcacgttc tcccggacgc ggagcgcgac aggggggctc gcgtcctcgc agccctgcgc
    17941 acgccgctca cggcggagat gctcgtcgtc gtgtgggccg gcttcgccgt gctgctcgtc
    18001 gcgctcgcct cccggaacac ctccctcctg ctcctctgtc tcgcagtcgt cctgaaggtc
    18061 ggagtccacg cgctcacggt gaaccagcct cgctacatct acccggtcac ggcgctgatc
    18121 cttctcgcgg cgccgctcgc ggcctgggtc ggacgcaggg agcgcggggg ccggaggctc
    18181 gccctcgcgg cggccgccgc cgtcctctgc accttcgctc tcgcgttcct cacgcggatc
    18241 gcggagcgct ggacgcgcgc ccacgacacg ccgatcgagt accgcttcgc gctgtgggac
    18301 ggatcgggcg gagagagact tcgatgccgc acggcgaacg ccggcatctg gtcgtggtac
    18361 ccgaagcggg acgagaaggt ggtgtcgttc ttccgacccg acgtcgcccc gggcgacgtc
    18421 gccgaggtcg cctgcgaggt gcccgccggc tcgtacgtcc cgcccgcggc gctcgagatc
    18481 gccgtcgcgc ccgaggtggg acggggcctc ttcgagctct cgctcggacg ggatggccag
    18541 gaccccgcgg tcgtggactc gagactgctg gccgacgggc ggcccatcga gctcccgctc
    18601 gttcccggag agctctcgcg gatcacggct cggctgaagg cggaggatcg agcgttcggg
    18661 aggggcctcg ggcacgcggc gacgatggcg ctgcgactga gggaaccgac acccgtcccg
    18721 ccgcccggcg cgacgcgcgt ccggtagcgg gacgtccggc gattccggtc gaaacgagtc
    18781 ctcgttccgc acgtccccgc gttcgcggcg gtatcatcga acgctcgagc gaatttctcg
    18841 acgcgcgagt cgcccctgct gatgcggagg tctgatgaac cggacccgtc tggctggctt
    18901 cctcgtttcg accacacttc tcctgagcgg cttcgcacgg gcggacggac ccgccctcgt
    18961 gcgtgtcgac cgggccgggc ccggagaccg agatgcgctg gtggccgccg gcgtccctct
    19021 cgtgctcgag caggagcgcc tcttcctcgc gctgggcgac gcgccggcga ttcgtcagcg
    19081 gcttcagtcg ctcgggagga ccggcgtcgt cgtcgacccc gacacgacgg gctcttcgta
    19141 ctacgtcgcc ggcctcaggc ccggcgccac ggcgggcgac ctcgcggcca cctgtggcga
    19201 gctcctcgtc gtggaggacg gctgggccct cgtgcgcacg ccgggtgagc tcggcgatcc
    19261 ctgcgtcgac tcgacgaagt ggttcctccg gagagtcccg atggagccgc tccggccgac
    19321 ggcgcccctc cccgagccgt acgcgtcgtg gcagcgcggc gagccgctgg ccctcgaccc
    19381 tcaccccgtc gtccagacga tcgtcgacga cctcacaccc gcgacgatcc aggcgaactg
    19441 ggaggcgatc gtcaacgtgg ccacgacccg gtactcgagg tcggcgggct gcgtgacggc
    19501 gaccgactcc gtcttcgcgg cgttccagac gctcggcctg aacccggtcc gccagaacca
    19561 cacgtccggg cacgcgccga acgtgatcgg gacgatcacc gggtacgcgc accccgagca
    19621 ggtgatcatc gtcatcggcc acatcgacga cgtgccgtcg agcggcgccg cccccggagc
    19681 cgacgacaac gcgagcggcg cggcgaccgt cgtctccgcg gcccaggcgc tggccggcta
    19741 cacgttcgag aagacggtca agctcatcgc ggtgaccggc gaagagcagg ggctctacgg
    19801 ctcagcgcac tacgctgacg aggcctgggc cacgggagag cagatcatcg ccgtcctgaa
    19861 cgccgacatg acgggctggg cgggcgacgg cctcccggcc tcgggggaga acctcgacgt
    19921 caacacgaac gccgcctccg cctggctcgg gacgctgctg gcggaagccg cgacggccta
    19981 cggcacgggc tgcgtcgtga acagcttctc ctgctcgtcg atggtctaca gcgaccacgc
    20041 cccgttctgg gactacggct ggtcggcgat ctgcgccatc accgacaacg aggggtcgtg
    20101 cgggcagggg gggagctatc cctactacca caccgtgaac gacacgctcg ccaactgcgg
    20161 caacccctcg ttctacgcgt cgacggtcaa ggcgttcgtc gccgcggcgg cccacctggc
    20221 cgtgccgctc tgcggcggcg gcgccttccc gccggtcccg acgggggtcg ccgcggcggc
    20281 cacgggcgtg aaccagatca ccgtcagctg gacgagcggc ggcgccggcc tggagtacga
    20341 ggtcctccgg ggccgcgggg gctgcgaggc cggcgacttc acgccggtcg ccacgacgac
    20401 gtcgtcccac t
//
LOCUS       DAOONL010000062        20169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2646_length_20169_cov_18.157453, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000062 DAOONL010000000
VERSION     DAOONL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20169
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2646_length_20169_cov_18.157453"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1078)
                     /locus_tag="PLP50_10435"
     CDS             complement(236..1078)
                     /locus_tag="PLP50_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52006.1"
                     /translation="MRVRLAGLPVEVGPDGRLSPAQKLALEQIESAPLLPGGVDRVLP
                     GAAAIRLATEPPWAGSLGISPTGEPPAVTCDGEQVRIGSQGFRAVVSPADGTADLYAA
                     TGVGHSVETVMRVLLSCRLPHLGGLPLHAAAIAMGTVGAVFYGVSGAGKSTLSECAPF
                     PVLSDEAVVAMPFDGRWEVSSSGFWGTLDRSTAPRGYLPLAGLFELHQDTSTRIERLP
                     ARRAFVSLLRVVTIPDSPPLWSAAVDALRRLVESVPVYSFGWTPAIPPWGAIREAIGL
                     PSAD"
     gene            complement(1075..2046)
                     /locus_tag="PLP50_10440"
     CDS             complement(1075..2046)
                     /locus_tag="PLP50_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52007.1"
                     /translation="MSALLELTYACSWRCVFCYNARHHDVARLSGDEWIGVLDDLRDL
                     GTLWITLTGGDPIAHPEFLRIAEAARDRAFGLRVFTNGALVDRAMAREIAGLGPLSVE
                     LSIHGATGDEHDRVTRSAGSFGEMTRAVDLLVENGARVVLKAVVTRLNEGSVDDIAAF
                     AARRGVELRLDVTLSPRDGGDKSPLGYRASAEGAERVLSRLGAAGELRAVRRSPGETN
                     CGLGQTTMAIDPEGNVYPCVQWREEALGNIRERRLGEIWRRSPLREHVTRISRAANDF
                     LVEAGAPLSGFRFCPALARQETGDPTRASSAHLELARAAARARGPEL"
     gene            complement(2109..2366)
                     /locus_tag="PLP50_10445"
     CDS             complement(2109..2366)
                     /locus_tag="PLP50_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPA52008.1"
                     /translation="MLRVHPRIAWQVVAGEAILLDLDNGRAIGLNETGTFLWPLLGTQ
                     DEGDLLESLVREFDVDRDMARRDLERFVALLRENGFIQDTA"
     gene            complement(2590..3135)
                     /locus_tag="PLP50_10450"
     CDS             complement(2590..3135)
                     /locus_tag="PLP50_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52009.1"
                     /translation="MRTNATAAFALATLAAIAVYPSRATCADIGKAGPKACSALIVTA
                     DKSEDTAWTKKKTFRATKILDVRVRAVIPSTVEFEAADVVTFRFTTPKGNLYQTIDVP
                     VRSSTKKGEAERRVPGYPFPLAVQQPKSTKVDGTPAQTVEARLPVGGTVIMESGLYGD
                     WIVQAFVGGARPCSATFVLQP"
     gene            complement(3146..6757)
                     /locus_tag="PLP50_10455"
     CDS             complement(3146..6757)
                     /locus_tag="PLP50_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPA52010.1"
                     /translation="MPFSALLDGGDGFLYGTTYTYTSTIFKIARDGSSFVKLRTLGPS
                     EGAHPVGTLVRGDDGFLYGTMSGGYGTGATDGTLFRIGTDGLGFEVRHSFATSPGSRP
                     VGGLLKASDGMLYGVTSEGGASGNGVIYRFNPALSDYTILHSLIGSEGRTPEAGLLEG
                     SDGWLYGTTSSGGEYWGGTLFKVDRTTGALATLRAFAPADGTAPIAALIRGSDGYLYG
                     TCSKSGLLGGGTVFRIAEDGSGFSVLAHLDASTIEGILPRTGLVRAGDYFYGTTEAGG
                     ATGSGTVFRVAVDGSQFATLHSFSLAVDGDRPIGPLHVHSDGLLYGVAFWGGPDGGGT
                     LFRLGTDGSAFEVIRSFDDSSCPDGCRPLGGLVNGGDGFLYGVTSGDSYYGGAAVFRL
                     NPATLAFESVAALQSGSEPQGSLLRKGSSFYGGTRQGGANYAGSIFRVDLPPGGSVTT
                     LHSFETSGPEGRYANGGLVEGSDGLIYGTTTAGGTGDRGTIFHIGVDGRSFSTVFSFS
                     SFDGRSPDGPLVVGDDGFLYGTTRGGGENVDAGAAYVFSPAAGVFSLLYSFDARDSQG
                     DAPTGPLLQTSDGRLYGTTSRTGGDTSSTGGTVYRLSPPAHVLPATAVSICRDQMGPT
                     VTATTAGPARTYQWGYRPLYGSVTALVGQTGPTYTVNGADFPDIYDGGVAEVVCTIRY
                     AGDYGESTSNPVAVTVNAACGDGSAVPPPVRVFTAATSNGTASTTLHWVNPATDVNAI
                     VIRYRSDGVYPTSADDAGSQLLCGMPDGPCGSAAAGAIGTFVHSSQTPGTTYSYGVFT
                     RSAAASSGGKFASAVPFNGPPKASWAYSTGATSVVPPGLLPTASGTPGALYTVSNDRG
                     LHAMTLGATGGDWPAGWKPLLMNAPSQGRPIPVNFPSFTVNGVRKVVYVTSQDGRVYV
                     ADALTGALLRSSAVLGDGIQHSPGAMLQDYGGDYDVLVVGTRNTLAALPMNELIGLNP
                     DTLAVLWRFDNGKGTNGIGIISGQPLVDYATNRVYFTSRQLAGGSSSTVWCLSFTESG
                     ATKVWEANVGSVDASVTLSNGTLYVGNLDGNVYALNASDGTFKWTTPFVTGDGPVRSL
                     VWSAGTGNRLYFSTTRKTWAITDNGAGTAPTAYWTPAPGVTSPSNPLVYAGSSYVGTG
                     TGQLLEFDVTVPGPPAPVGVTLTPHPAQIGRPTMDSVNGVIYVGTDEGAVHAASYPF"
     gene            complement(8263..8808)
                     /locus_tag="PLP50_10460"
     CDS             complement(8263..8808)
                     /locus_tag="PLP50_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52011.1"
                     /translation="MRTNATAAFALATLAAIAVYPSRATCADIGKAGPKACSALIVTA
                     DKSEDTAWTKKKTFRATKILDVRVRAVIPSTVEFEAADVVTFRFTTPKGNLYQTIDVP
                     VRSSTKKGEAERRVPGYPFPLAVQQPKSTKVDGTPAQTVEARLPVGGTVIMESGLYGD
                     WIVQAFVGGARPCSATFVLQP"
     gene            complement(8819..11662)
                     /locus_tag="PLP50_10465"
     CDS             complement(8819..11662)
                     /locus_tag="PLP50_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPA52012.1"
                     /translation="MSLSADGNTAIVGGFRDSGNLGAAWVFVRSGGIWTQQAKLVGTG
                     AVGSAYQGWSVSLSADGNTAIVGALYDNVSLGAAWVFVRSGGTWTQQGAKLVGTGAVG
                     SPHQGYSVSISGDGNTAIVGGADDNAYVGAAWLFSRSGGVWTQQGAKLVGANAVGMSS
                     QGVSVSLSANGSTAIMGGYDDDGGVGAAWVYAKLAPVITQTAGTNPSCNGASITLDAG
                     AGYQTYSWSPGGATTRTITVSPTSTTTYTVTVTDACSSASASHTQAVKPLVVAAAGAD
                     SEICAGEAVTLAGSGGAACSWSPSTGLSDPSSCTPTASPTQTTTYSLTVTQDGCASAN
                     VAQTTVTVSKPVFAGASGLSLVSFNDQCGLRLEWPAGTSSCPRSRSLVYNVYRSTNSS
                     FVPSSASLLERCLTTTFYNDMHADRDVTFYYVVRAENSGIGSAGPCNGGDEDDNLVRL
                     SGTVPAGTSCPTEPEDVAVLTARSGDGQNKLEWVNPSGSYSSTVLRYGTTGYPMSPTD
                     GLPLATVAGTAGAHDSFVHTPITNDVTYFYSAFVDAGGSYSSGRFVSGRPQATSGAVK
                     WAYNTSASSVVPSGLRPTVGEAPGALYTVSNDRGLHAMTLGATGGDWPAGWKPLLMNA
                     PSQGRPIPVNFPSFTVNGVRKVVYVTSQDGRVYVADALTGALLRSSAVLGDGIQHSPG
                     AMLQDYGGDYDVLVVGTRNTLAALPMNELIGLNPDTLAVLWRFDNGKGTNGIGIISGQ
                     PLVDYATNRVYFTSRQLAGGSSSTVWCLSFTESGATKVWEANVGSVDASVTLSNGTLY
                     VGNLDGNVYALNASDGTFKWTTPFVTGDGPVRSLVWSAGTGNRLYFSTTRKTWAITDN
                     GAGTAPTAYWTPAPGVTSPSNPLVYAGSSYVGTGTGQLLEFDVTVPGPPAPVGVTLTP
                     HPAQIGRPTMDSVNGVIYVGTDEGAVHAASYPF"
     gene            13932..15131
                     /locus_tag="PLP50_10470"
     CDS             13932..15131
                     /locus_tag="PLP50_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52013.1"
                     /translation="MTERTRLYGSILADHLRRHRQMAFVSGPRQVGKTTACRSAADTY
                     LSWDNADDRRLFLRGPSALAERLGLDRLREQRPVVVLDELHKHAKWKSLLKGFFDTWG
                     DRVRVLVTGSSRLDVFRRGGDSLMGRYLLYRMHPWSVGECLRTEAPESPIHRASAVSD
                     ADWGALLVHGGFPEPFLKRDIRFTRRWRSLRREQLSREDLRDVAHVADLGAMETLMEL
                     LSERTASQLVYAGLSREIGIALDTAKRWVDLLGRLHYGFLVRPWFRNVEKALRKEPKW
                     YLRDWSGVTDVGAHAETLVACHLLKAVETWTDLGFGDFELRYLRDKQKREVDFLVVAD
                     RKPWFLVEVKASGQPLSPSLSHFQGQTKAGHAFQIAMDLPFENVDCFKEKRPVVVPAR
                     TFLSQLP"
     gene            15313..17004
                     /locus_tag="PLP50_10475"
     CDS             15313..17004
                     /locus_tag="PLP50_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPA52014.1"
                     /translation="MKRAGLVLFLSLPLTPCAGAAPVPKGPVLEALEKGVARSFRLLK
                     EKGDPAPYFLSYHVTESEETSLSASRGAVRRDATYRTRLLDIDVRVGGYDLDSSRRLR
                     GGGYASNRVRPVSLPLEDDPVALEASLWLETDRRYRSAVERLQQVKADVAVKAAADDA
                     SADFSQEAPAKLVEPLPLSGVLPAGSAEKLKALSAVFEREGAVLDSEAALSVKTTRKS
                     FASSEGASVQHGDVTWRLTPFATTRADDGMDLYRFESFTARTPEGLPDEATLRKTAES
                     MTADLVALRAAPVLDPYTGPAVLSGKAAGVFFHEIFGHRIEGHRQKDETDGQTFTKMV
                     GQRVLPEFLTVYDDPTQKVSGSTDLNGFYLVDDEGVAARRTTVVDKGILKGFLMSRTP
                     VAGFAKSNGHGRGQPGMRPVGRQGNLVIEAAASVPNAKLREMLVEECRRQGKPFGLYF
                     ADISGGFTFTGRYLPQAFKVVPIMVYRVYADGRPDELVRGADLIGTPLVAFSRIVAAG
                     DEGGIINGYCGAESGTVPVSAVAPSLLTSQIEVQKKAKSSERPPILPPPAAEVKP"
     gene            17844..18772
                     /locus_tag="PLP50_10480"
                     /pseudo
     CDS             17844..18772
                     /locus_tag="PLP50_10480"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
     gene            18837..19181
                     /locus_tag="PLP50_10485"
     CDS             18837..19181
                     /locus_tag="PLP50_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPA52015.1"
                     /translation="MAQPDFVVEPDHGFLFLATEGEPIALKTLSLYVSRYVRGSGVAR
                     TGSCHLFGHAMATLMLENGADVRMIPAILGHVKPTTTEIYTRVAITKLNEIHTATHPA
                     ARLGPQGKKPDA"
     gene            19219..19449
                     /locus_tag="PLP50_10490"
     CDS             19219..19449
                     /locus_tag="PLP50_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPA52016.1"
                     /translation="MGELPRITFNPKVMGGKPCIRGMRVTVSTVVGLVATEHTHAEIL
                     ELYPYLEEEDIREALQYAAWRAGEVEIPLASA"
     gene            19446..19835
                     /locus_tag="PLP50_10495"
     CDS             19446..19835
                     /locus_tag="PLP50_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPA52017.1"
                     /translation="MKLLLDMNLSPRWAELLRQAGHDAVHWSTVGAATAEDVELMTWA
                     RTHGYVVVTNDLDFGAILAATQAEGPSVLQMRTAAVSPRSLGDRLRKALGRFEQDLLA
                     GALITVDERRERVRLLPIKLQRPEPGD"
     gene            19895..>20169
                     /locus_tag="PLP50_10500"
     CDS             19895..>20169
                     /locus_tag="PLP50_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52018.1"
                     /translation="MPTIEPSFPPLVTMELPGWELSWSELAPTGEGGDALARARLEAA
                     AEVRGRLKLETLSEHPAVAGLRRLFRRAGCDPTRYRSGSEALLRRVL"
BASE COUNT         3151 a         6819 c         7052 g         3147 t
ORIGIN      
        1 gatggagcag gtccttgagg ccgagccaga ggcgttcgat gagcgcgatg gagccttcga
       61 cgagctggtc gcatcaggat cacctcgccc gatgtgaggg gaccgtccaa aggctcaacg
      121 agcacctttc agcctcgctc gagcgtcggc gacatgctct ccccagtcag cgcgaagacg
      181 gcctcgaaga gcccggtcgc cgccgcgatt gactgtgcgt ggcacccgtt cggcatcaat
      241 ctgccgaagg gaggccgatc gcctcgcgga tcgcgcccca gggtgggatc gcgggcgtcc
      301 agccgaagga gtagacagga accgattcga ccagccggcg gagggcgtcg actgcggccg
      361 accagagggg tgggctgtcg gggatggtga cgacccgaag cagggagacg aaggcgcgcc
      421 gcgccggcag tcgttcgatt cgcgtgctcg tgtcctggtg aagctcgaac agacccgcga
      481 gcggaaggta gccccggggc gcagtcgaac gatcgagcgt tccccagaag cccgacgagg
      541 agacctccca gcgcccgtcg aacggcatcg cgacgacggc ttcgtccgag aggacaggga
      601 acggtgcgca ctccgacagc gtcgactttc ccgcgcccga aaccccatag aaaacggcgc
      661 cgacggttcc catcgcgatc gcggcggcat ggagcggcag ccctccgagg tggggaaggc
      721 ggcagctgag cagcactcgc atgaccgtct ccacggagtg gccgacaccg gtagcggcgt
      781 agaggtcggc cgtgccgtcc gcagggctca cgacggcgcg aaagccctgc gagccgatgc
      841 gaacctgctc gccgtcgcag gtcacggcag gcggttcgcc ggtcggagag atcccgaggg
      901 agccggccca ggggggctcg gtcgcgagac ggatcgcggc cgcgccggga agaactcggt
      961 cgacgccgcc cggcagcagc ggtgcgctct cgatctgctc cagtgcgagc ttctgggccg
     1021 gggagaggcg cccgtcggga ccgacctcga cgggaaggcc cgcgaggcga actctcaaag
     1081 ctccggtccg cgagcgcggg ccgcggctcg ggcgagctcg agatgggcgc tggatgctcg
     1141 cgtggggtca cccgtctcct gccgggcgag ggcaggacag aaccggaatc ccgagagggg
     1201 cgctcccgcc tcgacgagaa agtcgttcgc agcgcgcgag atgcgcgtca cgtgctcgcg
     1261 aagaggggac cggcgccaga tctctccgag ccgtcgctcg cggatgttcc cgagggcctc
     1321 ctcgcgccac tgcacgcacg ggtagacgtt cccctccggg tcgatcgcca tggtcgtctg
     1381 gccgaggccg caattcgtct ctcccggaga gcgtcggaca gcgcgcaact ccccggcggc
     1441 cccgagccgc gagaggaccc gctcggctcc ttccgcggac gcgcggtagc cgagaggtga
     1501 tttgtcgcct ccgtcgcggg ggctcaacgt cacgtcgagc ctcagctcaa cgcctcttcg
     1561 tgccgcgaat gccgcgatgt cgtcgacgga cccttcgttc agccgggtca ccacggcctt
     1621 caggacgacg cgggccccgt tctcgacgag caggtccacc gcccgcgtca tctcgccgaa
     1681 agagcccgcc gagcgagtaa cccggtcgtg ctcgtcgccg gtcgccccgt gaatgctgag
     1741 ctcgacggag agcggaccta ggccggcgat ctcccgcgcc atcgcgcggt ccacgagcgc
     1801 gccgttcgtg aagacgcgca gcccgaacgc ccggtcgcgg gcagcctccg cgatccggag
     1861 gaactcggga tgtgcaatcg ggtcgccccc cgtgagagtg atccagagcg tcccgaggtc
     1921 tctgaggtcg tccagaactc cgatccactc gtcgcccgag aggcgcgcga cgtcgtggtg
     1981 acgcgcgttg tagcagaaga cgcaccgcca cgagcatgcg taggtgagct cgagaagggc
     2041 gctcacgggt tgcgctgatg cggcagcccg gtcgagggcc gcggtgagcc tcgacgtcga
     2101 cggtgcgctc aagcggtgtc ctgaatgaag ccgttctctc tcagcagggc cacgaaacgc
     2161 tccaggtcgc ggcgcgccat gtcgcggtcg acgtcgaact cgcggacgag ggactcgagg
     2221 agatcgccct cgtcctgtgt gccgaggagc ggccagagga acgtgccggt ttcgttcagg
     2281 ccgatcgctc gcccgttgtc gaggtcgagg aggatggcct cgcccgcgac gacctgccac
     2341 gcgattcgcg gatggactcg gagcatggcc attcagctca ggtgaaacga cgggttgtcg
     2401 gagcaacgga acgggtccgc cttcgcgcac gccgcggcga ggttgcgctt cacctcgacc
     2461 tcctcgatcc aggcgatgtc cggcggagag tacggcctct ttccgcgttc actcgccgag
     2521 ggctccggtc gatttccgta caggtctggc aacgaggcgc tcctctcggt caaggcgttg
     2581 gctctgggct cagggctgga ggacgaaggt ggcggagcag ggtcgggcgc cgccgacgaa
     2641 ggcctgcacg atccagtctc cgtagaggcc gctctccatg atcaccgtgc cgccgaccgg
     2701 caggcgggcc tcgacggtct gcgcgggcgt gccgtctacc ttcgtgctct tcggctgctg
     2761 gacggcgagc gggaaggggt atccaggcac ccgacgctcc gcctcaccct tcttcgtcga
     2821 agacctcacg ggcacgtcga tcgtctgata gaggttcccc ttcggggtcg tgaagcggaa
     2881 cgtcacgacg tccgcggcct cgaactccac ggtggaagga atgacggccc tgacccgaac
     2941 gtcgaggatc ttcgtggccc gaaacgtctt cttctttgtc catgcggtgt cttcggactt
     3001 gtccgcggtg acgatgaggg cggagcacgc cttcgggccg gccttgccga tgtcggcgca
     3061 ggttgcccgc gaggggtaga cggcgatcgc ggccagcgtg gcgagcgcga aagcggcggt
     3121 agcgttcgtt ctcatcgcag cttcctcaga aggggtacga ggcggcgtgg acggccccct
     3181 cgtcggtccc gacgtagatc acgccgttca ccgagtccat cgtcgggcgg ccgatctgcg
     3241 cagggtgcgg cgtgagggtg actccgacgg gcgcgggagg acccgggacc gtcacgtcga
     3301 actccagcag ctgacctgtg ccggtgccca cgtaggagct gccggcatag acgagagggt
     3361 tcgaggggct ggtgaccccg ggagcaggag tccagtacgc cgtcggggct gttccggcgc
     3421 cgttgtcggt gatcgcccag gtcttgcggg tcgtggagaa gtagaggcga ttgccggtcc
     3481 cggcggacca gacgaggctc cggacgggcc cgtctcccgt aacgaacggc gttgtccact
     3541 tgaacgtccc gtcggaggcg ttgagggcgt agacgttgcc gtcgaggttc ccgacgtaga
     3601 gcgtgccgtt cgagagcgtc acggaggcgt ccaccgaccc gacgttcgcc tcccagacct
     3661 tcgtggcgcc ggactcggtg aagctcaggc accagacggt cgagctgctc cctcccgcga
     3721 gctggcggct cgtgaagtag acgcggttcg tcgcgtagtc gacgagcggc tggccgctga
     3781 tgatcccgat gccgttcgtc cccttcccgt tgtcgaagcg ccagagtacg gcgagcgtgt
     3841 cggggttcag gccgatgagc tcgttcatcg gcagagcggc gagggtgttc cgggtcccga
     3901 cgaccagcac gtcgtagtca ccgccgtagt cctggagcat cgcgcccggg ctgtgctgga
     3961 tgccgtcgcc caggacggcg ctcgagcgga ggagcgcccc cgtcagggcg tcggccacgt
     4021 agacgcggcc gtcctgcgag gtcacgtaga cgaccttgcg gacaccgttg acggtgaagc
     4081 tggggaagtt gaccgggatc gggcgaccct gtgagggggc gttcatcagg agcggcttcc
     4141 agcccgcggg ccagtcgccg cccgtcgcgc cgagcgtcat cgcgtggagg ccgcggtcgt
     4201 tcgagacggt gtagagagcg ccgggtgttc cgctggccgt gggcaggagc cctggcggaa
     4261 ccaccgaggt cgcgccggtg gagtaggccc aggaggcctt tggagggccg ttgaagggaa
     4321 cggcgctggc gaacttgccg ccggaggagg cggcggccga acgagtgaag acgccgtagg
     4381 aataggtcgt gcccggcgtc tgcgaagagt gcacgaacgt gccgatcgcg ccggcagccg
     4441 cgctcccgca cggcccgtcg ggcatgccgc agagaagctg cgagcctgcg tcgtcggccg
     4501 aggtcgggta gacgccgtcc gaccggtacc gaatgacgat cgcgttgacg tccgtggctg
     4561 ggttgaccca gtggagcgtc gtcgaagccg tgccgttgct ggtcgccgcg gtgaagacgc
     4621 ggactggcgg cgggaccgct gacccgtcgc cacaggcggc gttgaccgtc actgcgacgg
     4681 gattcgaggt cgactccccg tagtcgcccg cataccggat ggtacagacg acctctgcga
     4741 cgccgccgtc gtagatgtcc gggaagtcgg cgccgttgac ggtgtaggtc ggacccgtct
     4801 ggccgacgag ggccgtgacg gacccgtaga gcggcctgta gccccattga taggtccttg
     4861 ccgggccggc cgtcgtcgcc gtgaccgtcg ggcccatctg gtcgcggcag atcgatacgg
     4921 ctgtcgccgg caggacgtgc gcgggcgggc tcaggcgata gacggtccca ccggtggagc
     4981 tcgtgtcgcc gccggtcctc gatgtggttc cgtagagcct gccgtcgctc gtctgaagca
     5041 gtgggcctgt cggcgcgtct ccctggctgt cgcgcgcgtc gaagctgtag agaagcgaga
     5101 agacccccgc ggcgggcgag aacacatacg ccgcgccggc gtcaacgttc tcgccaccgc
     5161 cgcgcgtcgt gccgtagagg aacccgtcgt ctccgacgac gagggggccg tccgggctcc
     5221 ggccgtcgaa gctgctgaag gagaagaccg tcgagaacga tctcccgtcc acgccgatgt
     5281 ggaagatggt gcccctgtcg ccggtacctc ccgcggtcgt cgtgccgtag atcagcccgt
     5341 cggacccctc gacgagtccg ccgttcgcgt atctgccttc ggggcccgag gtctcgaacg
     5401 aatgcagcgt cgtaacggag cctcccggcg gcaggtcgac ccggaagatg ctgcccgcgt
     5461 aatttgctcc cccctgccgg gtgcctccgt agaacgagga ccccttgcga aggagcgacc
     5521 cttgcggctc ggacccggac tgaagggcgg ccaccgattc gaaggcgagc gtcgctggat
     5581 tcaatcggaa gaccgcggcg ccgccatagt acgagtcgcc gctcgtcacg ccgtagagga
     5641 agccgtctcc accgttcacc agtccgccca gcggcctgca gccgtcggga cacgacgagt
     5701 cgtcgaacga gcgaatcacc tcaaacgcgg agccgtccgt gccgagacgg aagagcgtgc
     5761 cccctccgtc ggggccgccc cagaaggcga cgccgtagag aaggccgtcg gagtggacgt
     5821 gaagcgggcc gatcggccga tcgccgtcga cggcgaggct gaaggagtga agcgtggcga
     5881 attgggagcc gtccaccgcc actcgaaaga ccgtccctga tcccgtcgcg ccgcccgctt
     5941 ccgtcgtgcc gtagaagtag tcgccggccc tgacgaggcc cgtgcgggga aggatgccct
     6001 ctatcgtcga cgcgtccagg tgcgcgagga cgctgaaccc acttccgtct tccgcgatcc
     6061 gaaagacggt gccacctccg agcagcccgg acttcgagca cgttccatag aggtagccgt
     6121 ccgatcccct gatcagggcc gcgatgggag ctgtgccgtc cgccggtgcg aacgcccgga
     6181 gcgtcgcgag cgctccagtc gtccggtcca ccttgaacag ggtccctccc cagtactctc
     6241 cacccgacga ggtcgtcccg tagagccagc cgtcgcttcc ctccagaagg ccggcttccg
     6301 gagtccggcc ttccgacccg atcagcgaat gcaggatcgt gtagtcgctc aacgccgggt
     6361 tgaatcggta gatcacgccg ttgccactgg ctcctccttc cgaagtgacg ccatagagca
     6421 tcccgtcgct cgccttgagg aggcccccaa cgggtctgga gccgggcgag gtcgcgaatg
     6481 agtgacggac ctcgaagccg agcccatctg tgccgatccg gaaaagggtc ccgtcggtgg
     6541 caccggtgcc gtagccgccg ctcatcgtgc cgtacaggaa cccgtcgtct ccacgcacga
     6601 gcgttccgac ggggtgggcg ccttcagaag ggccgagcgt ccggagcttg acgaaggagc
     6661 tgccgtccct tgcgatcttg aagatcgtcg acgtgtatgt gtaggtcgtt ccatacagga
     6721 atccgtcgcc cccgtcgagg agggcggaga agggcatgga tccgtccgtg ccggcgaacg
     6781 aatgaagggt cgtgaggcgt gccgaggcgt cgatgcgata caccgtcccg gcgtccgagc
     6841 ccccaccatt ctgcgtcgtg ccgtacagga acccgtcgcc gccgtccaac aggcctgcct
     6901 ggggtgacct tccgtcgcgc ccgccgaaag aatgaaagac ggagagggtg tagccgccga
     6961 ggccgtccgg cgtgagtttg tagatggcgc cggagcctgc gctcgtcgtc ccgtacaaga
     7021 cgccgcccga ggtttccagc agtcttccgc gcgagggtgc agcgggttgc gccagcgtcc
     7081 cgaacgaatg cagcacgtcc agggagttct gcgacgcagc cagaggggca atggcgaggt
     7141 gagcgaaggc gacgcatgcg gccgcgcggg aggtgcgggg gcggccaacc ttgcgaagcc
     7201 tggtcctgct catgacgaag agccgacgtc gggccacggt cgcgaaacga gactcctgat
     7261 acaaatgagc cccctctccg gaaaaggcaa caataaaggc aacgaatgag gataccagag
     7321 aggactccac cgaggcggat cgtcgttgac aagcccggcg cgaccctgtt caccgactcg
     7381 tcggcgagca ggatccggac ggcttcagcc gagccggaga agtcgtcgaa cccgtcgccc
     7441 ggcaacgcct gcgagggccg gacgcaggcc cggaatcgac tcagccagga cgcgcaggtg
     7501 cgcgtacagc cgctcgcgag cgacgaagcg cgccagacgc cgttgcacac tcggaggaag
     7561 gtcccgaagg gcgagcagcg gtcggccgcc ggggcgaacc acggcacaga cgcgcccgct
     7621 cacctgtgag gacggaacca cccccggcat gaccgacgag tctcccgcgt gaacgacgca
     7681 ctccttcagt ggctctcccc tcaagacggt gccgaggtac cggtgaatga ggggaccgcg
     7741 caccgtgtcg acgaggatca cgtcaccgta ctcgaggggt ccgttcaggg gctcgacccg
     7801 gaccgcccag cctcgctcga gcgtgggaga catgctccca cccgtcagct cgtggaccga
     7861 ctcgaagagc cgcgtcgccg cgtctcgccc gaagtccgtc gatcccgctc gtggctcttg
     7921 cgtcgcggcg agcgtcggcg tcgagggtgt gttcaggccg cgtccccgtt gtcgaggtcg
     7981 aggaggatgg cctcgcccgc gacgacctgc cacgcgattc gcggatggac tcggagcatg
     8041 gccattcagc tcaggtgaaa cgacgggttg tcggagcaac ggaacgggtc cgccttcgcg
     8101 cacgccgcgg cgaggttgcg cttcacctcg acctcctcga tccaggcgat gtccggcgga
     8161 gagtacggcc tctttccgcg ttcactcgcc gagggctccg gtcgatttcc gtacaggtct
     8221 ggcaacgagg cgctcctctc ggtcaaggcg ttggctctgg gctcagggct ggaggacgaa
     8281 ggtggcggag cagggtcggg cgccgccgac gaaggcctgc acgatccagt ctccgtagag
     8341 gccgctctcc atgatcaccg tgccgccgac cggcaggcgg gcctcgacgg tctgcgcggg
     8401 cgtgccgtct accttcgtgc tcttcggctg ctggacggcg agcgggaagg ggtatccagg
     8461 cacccgacgc tccgcctcac ccttcttcgt cgaagacctc acgggcacgt cgatcgtctg
     8521 atagaggttc cccttcgggg tcgtgaagcg gaacgtcacg acgtccgcgg cctcgaactc
     8581 cacggtggaa ggaatgacgg ccctgacccg aacgtcgagg atcttcgtgg cccgaaacgt
     8641 cttcttcttt gtccatgcgg tgtcttcgga cttgtccgcg gtgacgatga gggcggagca
     8701 cgccttcggg ccggccttgc cgatgtcggc gcaggttgcc cgcgaggggt agacggcgat
     8761 cgcggccagc gtggcgagcg cgaaagcggc ggtagcgttc gttctcatcg cagcttcctc
     8821 agaaggggta cgaggcggcg tggacggccc cctcgtcggt cccgacgtag atcacgccgt
     8881 tcaccgagtc catcgtcggg cggccgatct gcgcagggtg cggcgtgagg gtgactccga
     8941 cgggcgcggg aggacccggg accgtcacgt cgaactccag cagctgacct gtgccggtgc
     9001 ccacgtagga gctgccggca tagacgagag ggttcgaggg gctggtgacc ccgggagcag
     9061 gagtccagta cgccgtcggg gctgttccgg cgccgttgtc ggtgatcgcc caggtcttgc
     9121 gggtcgtgga gaagtagagg cgattgccgg tcccggcgga ccagacgagg ctccggacgg
     9181 gcccgtctcc cgtaacgaac ggcgttgtcc acttgaacgt cccgtcggag gcgttgaggg
     9241 cgtagacgtt gccgtcgagg ttcccgacgt agagcgtgcc gttcgagagc gtcacggagg
     9301 cgtccaccga cccgacgttc gcctcccaga ccttcgtggc gccggactcg gtgaagctca
     9361 ggcaccagac ggtcgagctg ctccctcccg cgagctggcg gctcgtgaag tagacgcggt
     9421 tcgtcgcgta gtcgacgagc ggctggccgc tgatgatccc gatgccgttc gtccccttcc
     9481 cgttgtcgaa gcgccagagt acggcgagcg tgtcggggtt caggccgatg agctcgttca
     9541 tcggcagagc ggcgagggtg ttccgggtcc cgacgaccag cacgtcgtag tcaccgccgt
     9601 agtcctggag catcgcgccc gggctgtgct ggatgccgtc gcccaggacg gcgctcgagc
     9661 ggaggagcgc ccccgtcagg gcgtcggcca cgtagacgcg gccgtcctgc gaggtcacgt
     9721 agacgacctt gcggacaccg ttgacggtga agctggggaa gttgaccggg atcgggcgac
     9781 cctgtgaggg ggcgttcatc aggagcggct tccagccggc cggccagtcg ccgccggtcg
     9841 cgccaagcgt catcgcgtgg agaccgcggt cgttggagac cgtgtagagc gcgcccggcg
     9901 cttcgccgac ggtcggacgc aggccggaag ggacgacgga cgaggcggac gtgttgtagg
     9961 cccacttcac cgcgcccgaa gtcgcctgcg gcctgccgga gacgaaccgc ccgctcgaat
    10021 aggacccgcc agcgtcgacg aaagcgctgt agaagtaggt cacgtcgttc gtgatcggcg
    10081 tgtggacgaa ggagtcgtgc gcgcccgcgg tccctgcgac cgtcgcgagc ggaagtccgt
    10141 ccgtcggcga catcggatac cccgtcgtgc cgtagcgcag gaccgtcgag gagtacgacc
    10201 cggaggggtt gacccactcg agcttgttct gcccgtcgcc cgagcgtgcc gtcaggacgg
    10261 ccacgtcctc cggttccgtc gggcaggacg ttccggccgg cacggtcccg gaaagcctga
    10321 cgaggttgtc gtcctcgtca cctccgttgc acggcccggc cgagccgatc ccggagttct
    10381 cggccctcac gacgtagtag aaagtgacgt cgcggtcggc gtgcatgtcg ttgtagaacg
    10441 tggtcgtgag acatctctcg agaagggagg ccgacgaggg gacgaagctc gagttggtcg
    10501 agcgatagac gttgtagacc agggaacggc tgcgtgggca cgagctcgta cccgccggcc
    10561 actcgagacg gaggccgcat tggtcattga agctgaccag gctgagcccc gaggcccccg
    10621 cgaacaccgg cttggagacg gtgacggtcg tctgagcgac gttggccgac gcgcagccgt
    10681 cctgggtgac ggtgagagag tacgtcgtcg tctgggtcgg tgaggccgtg ggggtacagg
    10741 agctgggatc ggaaaggccc gtcgacggag accaggaaca ggccgccccc ccgctcccgg
    10801 cgagcgtgac ggcctcgccg gcgcagatct cagaatctgc cccggcagcg gcgacgacga
    10861 gcggtttcac ggcctgcgtg tggctcgccg aggcgctcga gcacgcgtcg gtgaccgtca
    10921 cggtgtaggt cgtcgtggag gtgggcgaga cggtgatcgt cctcgtcgtg gcgccgcccg
    10981 gggaccagga gtaggtctga tagccggcac cggcgtcgag cgtgatggaa gcgccgttac
    11041 agctcgggtt ggtgccggcg gtttgggtga tgacgggcgc gagcttggcg tagacccatg
    11101 cggcgccgac acctccgtcg tcgtcgtacc cgcccatgat cgccgtgctt ccgttcgcgg
    11161 agagcgaaac cgacacgccc tggcttgaca ttccaacggc attggcacca acaagcttgg
    11221 cgccctgctg cgtccagacg cctccgcttc gcgaaaacag ccacgcggcg ccgacgtacg
    11281 cgttgtcgtc ggccccgccg acgatcgcgg tgttcccgtc gccggaaatc gaaaccgagt
    11341 agccctggtg cggcgatccc acggcccctg tcccaacgag cttggcgccc tgctgcgtcc
    11401 aggttcctcc gcttcgcacg aagacccatg cggcaccgag gctaacgttg tcgtagagcg
    11461 cgccgacgat tgccgtgttc ccgtccgcgg aaagcgagac cgaccagcct tgatacgccg
    11521 atcccacggc gccagttccg acgagcttgg cctgctgcgt ccagatccct ccgcttcgaa
    11581 cgaagaccca tgcggcgccg aggttgccgc tgtcgcggaa cccgccgacg atcgccgtgt
    11641 tcccgtccgc cgagagtgac acggagtagc cttgcttcgc tgctcccacg gctcctgtcc
    11701 cgacgagctt gcttccctgt tgcgtccaga ctccgccgct tcgcacaaag acccacgcgg
    11761 cgccggcatc gctgctgtca cccgagccgc ccacgatcgc cgtgttcccg tccgcggaga
    11821 gtgcgaccga atccccctgg tagctgaacg gcccggaccc tgtcccgacc agcttgtttc
    11881 cctgctgcgt ccaaacgccc ccgcttcgtg aaaagaccca cgcagcgccg gtgctactgc
    11941 tatccgcgcg cccacaaacg atcgctgtgc ttccgtccgc ggaaatcgag accgaccaac
    12001 cctgagacgc ccctcccatc gtccccgtac ctacgagctt cgcaccgtcc tgcgtccatc
    12061 cgccttcgct tcgggcgaac acccatgcgg caccggtacc caggttgtcg ttgtctccgc
    12121 cgatgagcgc catgttgcca tccgctgaga gcgataccga tctaccctgg cgcgacttcg
    12181 ccacggcgcc ggtcccaacg agcttcgccc cctgctgcgt ccagtctccc tcggttcgcg
    12241 tgaagaccca tgcagcaccg gcgtttccgt tgtcgcgggg gccgcctacg aatgctgtgt
    12301 tcccgtccga cgaaagggag gccgaggtgc cctggtacac cgtcgctccc acggcgccgg
    12361 tcccaacgag cttggtcccc tgctgtgtcc agacaccagc ggcgcgtgtg aatacccacg
    12421 ctgcaccggc gcctccgctg tcgttcttcc cgcccacaac cgcggtgctc ccgtccgacg
    12481 agagggagac ggagtatccc tgttgcgcgt ttcccgaggc tccacttccg atcagcttgg
    12541 ttccctcctg ggtccaaacc actccgcttc gcgtgaagac ccacgaggcg ccagcgcctc
    12601 cattgtcgcc atacccgcca acgatcgccg tgttcccgtt cgcggaaact gatactgaag
    12661 agccctggta cgccgatccc acggctcctg ccccgacgag cttggtgccc tgctgcgtcc
    12721 agaccccttc gcttcgtgtg aagacccacg cagcccctgc acccccgttg tcgtaatacc
    12781 cgcccacgaa cgccgtgttc ccatccgcgg aaagcgagac cgaccagcct tggtatgccg
    12841 atcccttgta ccccgtgccg acgagcttgg ccccctgctg cgtccacgcg cttccgcttc
    12901 gcgagaacac ccacgcggcc ccgcggcctc cgctgtcgtt gtatccgccg acgatcgcgg
    12961 tattcccgtc ggcggagagc gacaccgagt aaccctggta cacatagaag cccacggctc
    13021 ctgtgccgac gagcttggtc ccctgctgca tccagacgcc gccatttcgc gtgaagaccc
    13081 acgcggcgcc gactcctgcg gagtcgccag acccgcccac aagtacggtg ttcccgtcag
    13141 ccgagatcgc gaccgaggtg ccctgccgtg cggttccgac gtcccccgtc ccgacgagct
    13201 tgggcccctc ctgcgtccag gttcctccac ctcgggtgta gatccacacc gcgccgacgc
    13261 ctccgtggtc accgggccca cccactgccg ccgtgttccc gtccgcggaa acggccacag
    13321 agagtccgcg ttcgattgtc ccttccgcat cggaccccac cagcttgggc ccctgctggc
    13381 agaactgtcc gagcaagccg tgagaggaga gaagcgccgc gagaacggca acgcggaacg
    13441 tcaacatgat ctcggcctcc cgggccctct acctggacct acttttccga actcctcagc
    13501 gtagcgttac acctcgggcc gcgtcaacgc tgggttcctg atccttggag gagcttcacg
    13561 cgtcgaggcc tggcgtgaag cggcgtgcta gagacctccg aaatccgccc tgatgcgggc
    13621 actcacggcg ccctgcgccg gagagccgcc atgaagagct caccgacagg tcgtctgtgt
    13681 cagcccatca tcgcggcgag ggcgcgggtc tcgaggtcgg tcggcgaggc gctggtggag
    13741 tcgctgggca cgagacgggg catcgggtga gagtagcggc cttcacgctc ctcggcgacc
    13801 tactcgaagg agacgtcgca ccgatcgcgg cccttctccg ggaggtcgtc tctcgatccg
    13861 cactagaacc attctcgttc gattcgtaga acggtctagg ccattctacg atatatgcga
    13921 gaatggtcca gatgacggaa cggacgcggc tctacggctc gatcctcgcg gaccaccttc
    13981 ggcgccatcg ccagatggcg ttcgtgagcg gtccgcgcca ggtgggcaag acgacggcct
    14041 gccggtctgc ggcggatacg tacctgagct gggacaacgc cgacgaccgg aggcttttcc
    14101 tgcgcggacc gtcggcgctt gccgaacgac tcgggctcga ccgcctccga gagcagcggc
    14161 cggttgtcgt tctcgacgag ctccacaagc acgcgaaatg gaagtcgctc ctgaaggggt
    14221 tcttcgacac ctggggagac cgggtgcgcg tcctcgtgac ggggagctcg cgcctcgacg
    14281 tctttcgacg cgggggcgac agcctcatgg gccggtatct cctctaccgg atgcacccgt
    14341 ggtcggtggg ggagtgcctc cgaacggaag cgccggagag tcccattcat cgggcctcgg
    14401 cggtgagcga tgcagactgg ggggcgctgc tcgttcacgg aggatttccc gagccgttcc
    14461 tcaagcgcga catccggttc acgaggcggt ggcgttccct tcgccgggag cagctctcgc
    14521 gggaggacct ccgtgacgtc gcccacgtcg cggacctcgg cgcgatggag acgctcatgg
    14581 agcttctgag cgagcgaacg gcctctcagc tcgtctacgc gggactctcc cgggagatcg
    14641 gcatcgcgct cgacacggcg aagcggtggg tcgaccttct cgggcggctt cactacggct
    14701 tcctcgtgcg gccctggttc cggaacgtgg agaaggctct tcgcaaggag ccgaagtggt
    14761 acctcaggga ctggagcggc gtgacggacg tgggggcgca cgcggaaacg ctcgtcgcgt
    14821 gtcacctgct caaggccgtg gaaacctgga cggatctcgg gttcggcgac ttcgagctgc
    14881 gctacctgcg cgacaagcag aagcgggaag tggacttcct cgtcgtcgcg gatcggaagc
    14941 cgtggttcct cgtcgaggtg aaggcgtcag ggcaaccgct ctcgccgtcg ctgtcgcact
    15001 tccaggggca gacgaaggcc gggcacgcgt tccagatcgc gatggatctc cccttcgaga
    15061 acgtcgactg cttcaaggag aagaggcccg tcgtcgttcc ggccaggact ttcctgagcc
    15121 agcttccctg aagggaaacg gagttccctt ggggacgccc tcggtctcga gggcgcaggg
    15181 cgcgcagcag gtctcgccgt cctcgatcct tcttgcgcct cttctcccga cgttcttacc
    15241 ccccgcttcc gtaccatcaa agactgccaa tcgggtgact cgtccgtccg cgacggacat
    15301 gcaggagatg agatgaagag agcgggtctg gttctgttcc tgtcgcttcc gctgacgccg
    15361 tgcgcgggag cggcgccggt gccgaagggg ccggtgctcg aggcgctgga gaagggcgtc
    15421 gctcggtcgt tccggctcct gaaggagaag ggcgacccgg ccccctactt cctgtcgtac
    15481 cacgtgacgg agtcggaaga gacatcgctc tccgcctcgc gcggggcggt gcggcgcgac
    15541 gcgacgtacc ggacgcggct cctcgacatc gacgtgaggg tcggggggta cgacctcgac
    15601 agcagccggc gcctgcgcgg gggcgggtac gcgtcgaacc gggtccgccc ggtctcgctg
    15661 cccctcgagg acgaccccgt cgcgctcgaa gcgagcctct ggctcgagac cgaccggagg
    15721 taccggtcgg ccgtcgagcg gctccagcag gtgaaggccg acgtcgccgt gaaggccgcg
    15781 gcggacgacg cgtcggccga cttctcgcag gaggcgccgg cgaagctcgt ggagccgctt
    15841 cctctcagcg gcgtgctccc ggccggttcc gcggagaagc tgaaggcgct gtcggcggtc
    15901 ttcgagcgcg aaggcgccgt cctcgactcg gaggccgcgc tctcggtgaa gacgacccgg
    15961 aagtcgttcg cttcgagcga gggggcgtcg gtgcagcacg gcgacgtgac gtggcggctc
    16021 acgcccttcg ccacgacgcg cgcggacgac gggatggacc tctaccggtt cgagtcgttc
    16081 acggcgcgga cgccggaggg gctgcccgac gaggcgacgc tccggaagac ggccgagtcg
    16141 atgacggccg acctcgtcgc ccttcgcgcg gctcccgtcc tcgacccgta caccgggccg
    16201 gcggtcctct ccgggaaggc ggccggggtc ttcttccacg agatcttcgg gcaccggatc
    16261 gagggacacc ggcagaagga cgagacggac ggccagacgt tcacgaagat ggtcgggcag
    16321 cgcgtcctcc ccgagttcct gacggtctac gacgacccga cgcagaaggt ctcgggctcg
    16381 acggacctga acggcttcta cctcgtcgac gacgaggggg tcgcggcccg gcgcacgacg
    16441 gtcgtcgaca aggggatcct gaaggggttc ctgatgtcgc ggacgccggt ggcgggcttc
    16501 gcgaagtcga acgggcacgg ccgtggccag ccggggatga ggcccgtggg ccggcagggg
    16561 aacctcgtca tcgaggcggc cgcgtcggtg ccgaacgcga agctgcgcga gatgctcgtc
    16621 gaggagtgcc gcaggcaggg gaagccgttc ggtctctact tcgccgacat ctccggcggg
    16681 ttcaccttca ccgggaggta ccttccgcag gcgttcaagg tcgtgccgat catggtctac
    16741 cgcgtctacg ccgacgggcg gcccgacgag ttggtgcgcg gcgcggacct catcgggacg
    16801 ccgctcgtcg cgttcagcag gatcgtcgcg gcgggagacg aagggggaat catcaacggc
    16861 tactgcgggg ccgaatcggg cacggttccc gtatcggcgg tcgccccgag cctcctgacg
    16921 tcgcagatcg aggtccagaa gaaggcgaag tcgtcggagc ggccgcccat actcccgccg
    16981 ccggcggcgg aggtgaagcc gtgaacgcgc cacgcatcgg cgcggccctc gcggtcgccg
    17041 cagcgcttct ctccggcccg atcgccgcgg ccgatgctcc cacgcccggg cgcgacccgg
    17101 tcctctcggc gctcgaggcc gagctcgctc gctcggtgga gcggctccgg ctcgacggcg
    17161 tggagaggcc gtacttcatc tcgcacacgg tcaccgacac caacgtccgg cgcgtcgagg
    17221 cgacgttcgg ggccgtcgtg aggtcgaccg tcgagaagtc tcggcgtctc aggaccgacg
    17281 tccgcgtcgg aagctacgac gtcgacagct cggagttcct ggccgggcgc tcgccgtacg
    17341 agcggttcga cgccttcccg cggatgctcg tgaccgacga cgacgagatg gcgataaggc
    17401 acgaggtctg gctcgcgacc gacgaggcgt acaaggcggc cgcggagcgt ctcgagcgga
    17461 agaaggccgt gctcgcgaac cgggtggaga aggacccggt ccccgacttc agccgcgaga
    17521 ggccgttcgt ctcgcttgcc ccgcgccgcg ccgcacccga gccgggggag tggccggagc
    17581 gcgtgaagcg cctctcggcg atcttccgcg agctccccga cgtccaggag tcgagcctcg
    17641 tcctgacgat cgcctcggtg aaccggacgt tcgtcaacag cgaggggacc cgtgtccgcg
    17701 tgccgtccgc acgctggtcg atcgaagtcc aggcctcgac gcaggccgag gacggcgcgc
    17761 cgctgaagcg tgtgttgagt ttccacgagg tcgagccgtc gcgccttccc acggaggccg
    17821 tgcttggcgc ggcgatccgg aagatggccg ccgagctgag cgcgctgcgc gtcgcccccg
    17881 tcctcgagag ctacacgggg ccggtgctct tcgcggacga ggcggcgccg acgctcttcg
    17941 ccgacctctt cgcgccgcag ctctccggcc ggcgaccgcc gctgacggcc cggcccgcga
    18001 cgggcggggc ggagcagggg tccgacctgg ccggccggct gggccggctc gtcctccccg
    18061 tcttcctcgg cgtcgtggac gaccccacgc tcgcgagcga cggcggccgg accctcctcg
    18121 ggtcgtacga cgtcgacgac gagggcgtgc cgcccggacg ttgacgctcg tcgaccgggg
    18181 cgtcctgaag acgctcgcga tgggacggcg gccgcgcaag gagatcgcgg cgtcgaacgg
    18241 ccacgcgcgc ggatcgagct acttcccccc ggcgccggcg gtgagcaacc tcctcgtccg
    18301 cgcctcgtcg ggccggagcc acgaggagct gaagaaggag ctgatccagc aggcgaccgc
    18361 ccagggcctg ccgttcgcgc ttctcgtccg cgcgctcggg ccgggccgga cggaagagac
    18421 ggatatgttc gggatgtcgt tcggcgaggc tcgaccgcgc tcgccgctcg gcgatccggc
    18481 cctcgcctgg aaggtcttcc cggacgggcg tgaggagctt gtgcgcggcc tctcgttcgg
    18541 tccggtcgcg atccgcgatc tccgcgacat ctcggccgcg gggaccgaca cgtgggtcct
    18601 ctcccgcccg agctccgtcg gcgcccatgc tccggtctcg gtcgtggccc ccgagcttct
    18661 cttcccggat ctggagctgc gccgcgacac gagcacggcg ccgaaacctc cgctcctctc
    18721 gaacccgtgg ttcgacaaga gcgcggcggg aaagggagcg aagaagaggt agccgtcgtc
    18781 gtcgtgcgtc acgactccgc gccgttgtcc gcgaggttag tcaccgacac gcgatcctgg
    18841 cccagcccga cttcgtcgtc gagccggacc acggcttcct cttcctcgcg accgaggggg
    18901 agccgatcgc cctgaagacg ctcagcctct acgtgagccg gtacgtccgg ggctcgggcg
    18961 tggcgcggac gggctcgtgc cacctcttcg ggcacgcgat ggcgacgctg atgctcgaga
    19021 acggggccga cgtccggatg atcccggcga tcctcgggca cgtgaagccg acgacgacgg
    19081 agatctacac ccgcgtggcg atcacgaagc tcaatgagat ccacacggcc acccacccgg
    19141 cggcccggct ggggccgcaa ggcaagaagc ccgatgcgtg aatcccgaac tcgaccgaac
    19201 cgctatcatg agggggccat gggcgagctg ccgcgcatca ccttcaaccc gaaagtcatg
    19261 ggcggcaagc cgtgcatccg cgggatgcgc gtgacggtga gcactgtggt cgggctcgtc
    19321 gccaccgagc acacgcacgc cgagatcctg gagctctatc cctaccttga ggaagaggac
    19381 atccgggagg cgctgcagta cgcggcctgg cgcgcgggcg aggtcgagat acctctggcc
    19441 tcggcgtgaa gctcctgctc gacatgaacc tctcgccccg ctgggcggag ctcctccgcc
    19501 aggcgggcca cgacgccgtc cactggtcga ccgtcggtgc ggccacggcg gaggacgtcg
    19561 agctgatgac ctgggcgcgg acgcacggct acgtcgtcgt gacgaacgac ctggacttcg
    19621 gggcgatcct ggccgcgacg caggccgaag ggccgagcgt cctccagatg cggacggcgg
    19681 ccgtctcccc gcgctccctc ggggatcgcc tccgcaaggc gctcgggcgc ttcgagcagg
    19741 acctcctggc tggagcgctg atcacggtgg acgaaaggcg cgagcgggtc cggctgctgc
    19801 cgatcaagct ccagcgcccg gagcccgggg actgacgggg ggagctatcc cggggcgagg
    19861 agcgccggtc gcggagtcgg cgagaatcgt cgcgatgccg acgatcgagc cctcctttcc
    19921 gcctctcgtg acgatggagc tccccgggtg ggagctctcg tggtccgagc tggcgccgac
    19981 cggagagggc ggggacgcgc tcgcccgggc gaggctcgaa gccgcggccg aggtgcgcgg
    20041 ccgcctgaag ctcgagacgc tgtcggagca cccggcggtc gcggggctcc gacgcctctt
    20101 tcgccgcgcg gggtgcgacc cgacgcgcta ccggtcgggt tccgaggcgc tgctccggcg
    20161 tgtgctgaa
//
LOCUS       DAOONL010000063        19816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2737_length_19816_cov_18.895906, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000063 DAOONL010000000
VERSION     DAOONL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_19816_cov_18.895906"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLP50_10505"
     CDS             complement(<1..211)
                     /locus_tag="PLP50_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HPA52019.1"
                     /translation="MSRFDNEIRVRPSVLDRLIDYEPEYSREAPASRVKNLRLLKQSV
                     RRDLEWLLNTRATAVVPPELKEVWSS"
     gene            complement(285..1778)
                     /gene="tssC"
                     /locus_tag="PLP50_10510"
     CDS             complement(285..1778)
                     /gene="tssC"
                     /locus_tag="PLP50_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HPA52020.1"
                     /translation="MSSGAEKAKAQAIAREQEGEVDLLSKIIDEGRLAQHESQTQQAR
                     DMIAEFVQQAMQGQMVLSRNLESSVQARIAEIDRLLSAQLNEIMHSEPYQKLEGSWRG
                     LHHLVYESETSVMLKIRVLNVSKKDLLKDLERAAEFDQSAVFKKVYEEEYGTFGGQPF
                     GALIGDFYFSNHPQDMLLLEKMAQVAAAANAPFIAASAPQLFGWESFQEMTEVRDLAK
                     IFDRTEYAKWRSFRDSEDSRYVGLTMPRTLMRLPYGKETVPTETFNFEEDVDGKDHNK
                     YLWGNAAYSFGTRLTEAFAMYGWCVAIRGVEGGGLVQGLPVHTFQTDEGDVALKCPTE
                     IAITDRREKELSDLGFIPLVHCKNTDYAAFFGAQSAQKPKKYDLDSANANARLSTMLP
                     HIFAVSRFAHYLKAMMRDKVGSFMSRKDCEMFLNQWISNYVSPDDTASADTKARYPLR
                     DAKIEVAEIPGKPGAYRAVAFLRPHFQLDELQVSLRLVADLPASARK"
     gene            complement(1771..2307)
                     /gene="tssB"
                     /locus_tag="PLP50_10515"
     CDS             complement(1771..2307)
                     /gene="tssB"
                     /locus_tag="PLP50_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HPA52021.1"
                     /translation="MVKKESIQQKLKRVRPPRVQITYDVEIGGAIELKELPFVVGVLG
                     DFSGQPEQPLPRMKDRKITEIDRDNFDQVLAGMTPRVATSVENKLAGDGSKMAVDLKF
                     QSLEDFEPDKVVQQVEPLRKLVEARQRLSDLLSKADGNEKLEKMLNDVLQNAGSQQQL
                     ASALGLSPEGPKKGDGNE"
     gene            complement(2360..3478)
                     /gene="tssA"
                     /locus_tag="PLP50_10520"
     CDS             complement(2360..3478)
                     /gene="tssA"
                     /locus_tag="PLP50_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HPA52022.1"
                     /translation="MSSEAPLLDFERLLAPVSDDRPSGESLQYSGLYDEIREARRADD
                     QLEQGDWKRELKVADWDEAVALATDALQTKTKDLQVAAWLAEGLTMLHGFAGARDGIR
                     LMKGLHADYWDTCYPEIDEGDMEGRANVLSFFDRQVALALRKAPMTRAPGMNYSWLEW
                     SESRPFDVPEATAGPLDHDAQARLAELKRQAAEENKTSGEDFRKAKSSTPKVFYEEAS
                     KALAVAWDEYLALDRVMDERFGRQTPGLGELKVALEEVRRVVDGILKEKRALEPDPSA
                     PGAADAEEAGDVPAGAVYRAAGPAGPLRSRGEALARLGEVAEFFRQTEPHSPVSYLVQ
                     RAISWGQMPLETWLADVIRDTNVLDGIKETLGIRPGTE"
     gene            3607..4416
                     /locus_tag="PLP50_10525"
     CDS             3607..4416
                     /locus_tag="PLP50_10525"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HPA52023.1"
                     /translation="MPTPKELLDSGNLKAAIEALTQEIRDSPADPQKRTFLFELLAFA
                     GEWDRAEKQIVAAAGADAPSQMAAGVYRGLLQAERERARVFAGKARPHFLRPVPPGVE
                     KTLAALGRISEGDLAVARTLLDEAEEERPALAGRRGESAFEDLRDFDDRIGGVVEMVF
                     QGKYTWFPLEFARSLEPNAPKKLRDLIWPGARITADDGTSADVFLFALYPGSAAHEDD
                     LVKLGRMTDWKNLSEDLQAGLGLRSILVDGEDVSLLELGPVEFDEREPLPS"
     gene            complement(4538..5029)
                     /locus_tag="PLP50_10530"
     CDS             complement(4538..5029)
                     /locus_tag="PLP50_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HPA52024.1"
                     /translation="MGGLVDYFLKIDGIEGESADEKHKGEIDVLSWAWGASQSGSMQY
                     GGGGGAGKVQMQDFSFTMRINKATPKLVLHCANGAHLKSAILTARKAGKDQQEYLKLT
                     FTDLLVSSYQTGGSGDSDVVPIDSISLNFAKIEFEYKEQKADGTLGGAIKTHYDLKAN
                     KGG"
     gene            complement(5138..6163)
                     /gene="tagF"
                     /locus_tag="PLP50_10535"
     CDS             complement(5138..6163)
                     /gene="tagF"
                     /locus_tag="PLP50_10535"
                     /inference="COORDINATES: protein motif:HMM:NF021393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HPA52025.1"
                     /translation="MAAWSLISKLKEGFAGADGPPRMETCALHLYGKLPIYKDFISSG
                     LTEEGAKEFRDWLGNGFSRRWANDDEYREAEIPLHTFLLPLPGGRRSVAGALWGSHDE
                     GGLRRFPFTLFSVVPQGKAIADPLVALSYLEVFEERATQIRRTYAAGRTLASFYEAYR
                     GAKIEVPVKRPAKVAEGVEKEAREVTLGDFAESLLGEGAAESWPSFLARLRAACDAPA
                     GDGAGAVRVPVGNLLPATVQMQLWLAWLETEGAFGRRTPSGVLVCRSGGRSRAVLFFR
                     ELRAEDFLLLHPERSDHEFVEEAVPAAPESAPKPADGGESPETPSRPKGWDRPLAAFL
                     RPEGRET"
     gene            complement(6243..10514)
                     /locus_tag="PLP50_10540"
     CDS             complement(6243..10514)
                     /locus_tag="PLP50_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HPA52026.1"
                     /translation="MYEFLDLLYRLPKPIKTLGIVVLLSPVCLVIARALGFGHLWWAV
                     IGGLILIALLIAAFDVFVKKGDKARGGAFEKDLARDAQGGAGASKQEVREAVKELSQK
                     WTESLAQLKATGLQVYSLPWYLLIGEPQSGKSTTLKNSGLEFPVGGDGLSGSGGTRNC
                     DWWFANEAVILDTAGRFTFQEEAAPDATEWSTFLKMLKKYRKECPINGVLVVIPATSL
                     LEDTPEEQDKKAANIRTKLLHLQRVLEIRFPIFIMVTKADRILGFSEFFSKLDPVDQR
                     QLVGWSNPVGPDKPYVLATFDEAYEEILTRVHKLRMKFTANEEIVQNVDRIFVFPEEL
                     RALGNSLKRYFQGIFQETRYDDAFVFRGFYLSSGVQQGKPIARATRELLGSSAEGIVE
                     NLEQIFKRSRAFFIKDFYEKKVFPEQGLIARTKAALEREKTTRWVIRAVTVFLPIFCL
                     LGMILPYVAMRKFVKPMRETALKAQACVEKKEACSIGEAYGIAESIYKHQAAIRQRPF
                     LFALFFQRASSGELFRLLGKINQRLTYERVVNPLMAEGEARMARLDWAGPYDYRVFFS
                     ALDGHMKWWAVKKGALGAEQKPLVAASELKLIPLLAFLQNTKGTAGTAATKEIDEWVA
                     SIGSSGTPDEILAALPSDPPKDPELIRVPDPAGALKKFEEYWSVANLARWDFRLNQLL
                     AQYVQRYSELLKVEDPSSRGYLDRVAATGKEFRRVYDDTTKHLATVRPGSKEFPGAGP
                     DDWKRNFDADYTQLMRFTTVVPGVVNEQARSRIKGQMDSDWKNLEVQVLAYAYLVVPD
                     AQDPAKKGWSPSAAAISPMVADATAFADLAAFEATENPAAVVEEAARKSTPADQKATL
                     EAFTKKQVDAKAKAVANLTAIPAIPAEQKTLWQDTALATFVPRTADLALVYRVLPYTQ
                     QFLDRSVGPACLENVCFTRNHAQQMVPLGVSYISFAEASSISTRPDVDALAQGHAEAM
                     LDYLRRYVDRFAGRGGGGGGGFIVPGSALRASSWREFQAAITRWSPESQGGDAPVTDM
                     SGALAFDDVQTFAASNRRLASILPYFKGKAAPRRSTGPAKAPPELVTAANLFKNNVVP
                     LPEQELKAWRQLSLGQDGVSLRGFHAFSRNPAVRRSSYGRQLIAVENRGANLIRDAIQ
                     PSFQQAAEPVVRKIREQCLGQFPFVRESQLRQERVAYAAGSSLKPGGSGNTATYRLDL
                     ATIGQNHFSGILTDVGALADEYALNPILWGWEPQFDFVGDAGRGLLAVGRGWQLFLFG
                     GGRSTSVIKEHKVEIRPLQFSPSPGKRFMGDRINSLFLFDRSVVVRPSTDFKTGRQPS
                     PYVWRLGPADAPMSITGRDEQSGSGWTGSLEAYGGPLRFFYFVRVASEERDRRDDSDD
                     PERAREKEKIWTIRVVIPNAVQRTEPLEGLFELAFEEPMPPILPN"
     gene            complement(10522..11199)
                     /locus_tag="PLP50_10545"
     CDS             complement(10522..11199)
                     /locus_tag="PLP50_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HPA52027.1"
                     /translation="MDYTTELFKVAAKEFLFLSSFRQKVRKGVRADIEDVAADLDEIF
                     REQASAVRSDPRLQALYDQARYPLVVLADEILIHSGWEFARDWQDRLLEEKYFRSNIG
                     GDQYFAIAKELRPEDVELGSIVYAGLALGFRGKYRERPEKLAEVRKNTYRLLSEYLAS
                     QSDKITPEAYTVMATSGKRLNPVVTLGRVAIISFSVLIGFWILTFIAWATLVGQVKSV
                     AEAMGVI"
     gene            complement(11201..12559)
                     /gene="tssK"
                     /locus_tag="PLP50_10550"
     CDS             complement(11201..12559)
                     /gene="tssK"
                     /locus_tag="PLP50_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HPA52028.1"
                     /translation="MKKTPQVLWAEGMFLRPHHLQTAERYREEALQREIQRIQPFYFG
                     LQSLDVAPDQLENFVFEIRDVSLKLKDGTALSSDSTLRISPRSFKNELDKASGRMRVF
                     VGVPVMRETAPNTFAPGEGPAGVDRRYVAENAEALDENTGGNPQPVGVRRCNGKLFFG
                     AESQEGYECLEVAVVERSGSGRNFPVLSREFIPSLTELGASRTIQALCDGVTNRIEAK
                     HRLLLTDVAQGRLAITSDGTSGWQAIIKLQILGSFLFLFQQLTKIPRIHPFHVYTEFA
                     RLAGELSIFDDGKVPVRIPVYDHEKLGPCFHETCRVIEALLEKIVASRFVKVDFVLRE
                     DLLVADLLEEWLASQTEVFLAIEAEMEEKDLRGRIVTMKLGTVRDIPVLRQRRLFGLD
                     LELLKRTPAGLPQREDYFYFKIEKEGPYWANVVRDRNVALSDALDPKLKFSLYIITKP
                     GS"
     gene            complement(12569..13051)
                     /locus_tag="PLP50_10555"
     CDS             complement(12569..13051)
                     /locus_tag="PLP50_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52029.1"
                     /translation="MTKTPSRFLLRIAACGLLLGAFPALTGCASKVGTISFRCGAQIN
                     GGLALAVDVIRATEEESRKIQEMGEKWFYDQTRFNIQGQNRIQTVTFTTGEGAGPCDK
                     TVSVPFSKGERYLVLIADYKFQNPDPTKHVVVLSREKWVGQKILVSVLERELAVERRW
                     "
     gene            complement(13048..15180)
                     /locus_tag="PLP50_10560"
     CDS             complement(13048..15180)
                     /locus_tag="PLP50_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPA52030.1"
                     /translation="MSPLLRGVRVSGPPRSLTLEVWLGPMEGLRYAAEARQAMQLVRI
                     GRLPFDRQTKERNHLVLSVSAGVSGSHAEVRFAQGRIFLRDLGSSNGTFAAQQAVTTE
                     TEVKSGEIFLVSLTPVQVYLTSPLAAPPPFEPLAVTHWEGASVEPLLTAAAECAAGRR
                     EGYVDTRHLLDAILALGDPEIEELLASGGVRPAEVRQALFHKPFFRDTLGWIAEILVK
                     PVNLTGSFETAVVSPRVARLLDGTRTAVPDGVSGDEAVRWAARSVLAGLVADRFGPVG
                     DWLTACGITSVPEGFVGESGAKAPAARPAERELTGRASMKGTAGKGRATTLLTEEVGE
                     ATAKGGPSPLSRTLPPPPPPPPPASPESSAVAPAPARRHEPEPEAAPAARPEPPREPE
                     PLPAPPPSAVPAPGPAPFAWGAPRTPAEILLDGRARELADELFALAGELRFGTSDDRR
                     RQLKKRVEKELAPLPRESRTRFLELLRRHFPVLPAGAAVDPEVTRLRRKLEEVERKRV
                     DESPSKGVERRKAQASTASSGPLPLKQLVHGEELAQADRNVVVLRRVIEYALAMEGMT
                     LGLIQSLTMPGNETMAFKLPGFKETLRHFLHAIEQGKPVSIQRVEEYLEELKKWQVAI
                     LAAFHQAPGEWFDRLWKRINPVTIESAPRSAGWKLRGEAAEWWDLYKIAVRDLSPDLV
                     NDQILQGVARLSKEELEKLRKTQRGEHS"
     gene            complement(15272..17404)
                     /locus_tag="PLP50_10565"
     CDS             complement(15272..17404)
                     /locus_tag="PLP50_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52031.1"
                     /translation="MKGLPTARLLPPGDAPSPFGLHLVLGPLAGGAEEGPVPLPLSAD
                     EGTSRVYLGALKGDADSVVELVAVKLLPNVLPDALAESAARPTNRTLVARFDAERERM
                     RALRAESPYFPRLLQPDGGPEGTLPALVYDKPSRTFFIPPCASCGRPLELLTDEQFLR
                     ERRLPSYLESLHRLVGCAGCATTDRVVAVFSYSPLPGEADVPVGGPDELLKGLSRALL
                     RDWDEGQVKAFASRESAEEALRLRAAGGGSLNDFAARWSPFSFCPSPFLVSGLSPVRW
                     DEWADFVGGRTEEDLVSPAAPETLETESTRRRIEWLRASLPPTGRFLYGHDGSGVDAI
                     EVLYLKMTAFRQLAAGLLAFYRATGQPHLDLHPGHVLLDSYGAGASLPALWTFQVRLH
                     GLSAASRTSSFGVEVVVPPPEPMFPYAAPEILEFRLASRRPGDVYLSEIEPADAGLGV
                     KVEGRLSDPNGLFPRPEEPDWIHVTFPDEALGLGIDALVVRRKPDVPASYEELTFVSE
                     PVELDDAAVKRLRRSFGVRLPGARYKVYPRFGAPSDLHSLGVLLLRSLAGGERTELPE
                     LLQGVQRAAATAARSKEGDALARVKAALAAEASTATLLDRSRVFWKEEDRVPGRPNAI
                     PPPLFERALLLALRLLTRIPGFSIATGPGDFVAEDPTGRVEQVLRETDEVVAELRALL
                     VHRQPLHFEIQDVLAELLEAESETAPRA"
     gene            complement(17485..19536)
                     /locus_tag="PLP50_10570"
     CDS             complement(17485..19536)
                     /locus_tag="PLP50_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HPA52032.1"
                     /translation="MTPIRRILASGLLAVALPGAAAEGVRPVSSPRHERAVLPGAPGP
                     NRLDVDVPLLAGSEARSRSGLLSDLRFHDAAGREVPYLLISPGRSEPEWLPATLLPIA
                     ATKTASGFEADLGALRCVDRLRVHGIPAPFLKRLRLEGSGDRARWAVLVAEGTLFDLP
                     EEGLARTVVAFPAGEHRFFRLVWNDAKSGRVPTPSRVEARAAGTGGTPETLRAEVSFE
                     RRESEPGRSRYRLRLPAAGLPLSALEVGVRSGNVLREARVLESRLADGRLVPYELGAA
                     TLRRAEREDAAAAEMTITLTPPTEAELELVVEDAGNPPLELASVTALFEGLPFVYFES
                     PDGAPLAARYGAPGLERPRYDLEAARPAVTRRGAPPPASASWGEERPRPALVAEASAP
                     PAVPAGAALERDAFRVSRPIPPGPPGLTALRLDAAVLAASPSLADVRIASADGRQVPY
                     LLETLGEPLVVRLPAPQRVDDPRPRGGEGTGGRLSFHRIELPHEDLPASRLELTTTAR
                     VFARSVVLLRERLGGRPETAGTFTAITSSSWQSADPAREAPRLVLELPPGTGGTLFVS
                     IDDGDNAPLPLSRAALLLPAHRLRFVREDGPLALLAGRPGLAVPRYDLELLAPRLLGA
                     PATEVELAPEALPAVAPSAGSRVFWVALVGAVVALLVLLARLLRRPTGSAGSEAGGGE
                     G"
     gene            complement(19533..>19816)
                     /locus_tag="PLP50_10575"
     CDS             complement(19533..>19816)
                     /locus_tag="PLP50_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPA52033.1"
                     /translation="LARGTSLAQDLAYTLAWAVFGVGLLAAGIVGKSRAARAAALGLL
                     VVTVLKAFLHDLGRLGGLYRVASFVGLAISLSLVAIAIQKFVLRPREKE"
BASE COUNT         2849 a         6870 c         7156 g         2941 t
ORIGIN      
        1 tggaggacca gacctccttc agctcgggcg gcacgaccgc cgtcgcccgc gtgttgagga
       61 gccactcgag gtcgcgccgg acggactgct tgagcaggcg caggttcttc acccgcgagg
      121 ccggggcctc gcgcgagtac tcgggctcgt agtcgatcag ccggtccagg accgacgggc
      181 ggacacggat ctcgttgtcg aaccgagaca tctcgggcgg actcctcggg cggacgagcg
      241 gtggatcggc cggacggcga agaccgccgt ccggccgggt ggggctactt gcgcgccgaa
      301 gcgggcaggt cggccacgag gcggagcgag acctgcagct cgtcgagctg gaagtggggc
      361 cggaggaacg ccaccgcgcg gtaggcgccg ggcttgccgg ggatctcggc gacctcgatc
      421 ttcgcgtcgc gcagcggata ccgggccttc gtgtccgcgg acgccgtgtc gtccggagag
      481 acgtagttcg agatccactg gttgaggaac atctcgcagt ccttccggga catgaagctg
      541 ccgaccttgt cgcgcatcat ggccttcagg tagtgcgcga agcgcgagac cgcgaagatg
      601 tgcgggagca tcgtcgagag gcgggcgttc gcgttcgccg agtcgaggtc gtacttcttc
      661 ggcttctgcg ccgactgcgc cccgaagaag gcggcgtagt ccgtgttctt gcagtggacg
      721 agcgggatga agccgaggtc cgagagctcc ttctcgcgcc ggtcggtgat cgcgatctcc
      781 gtcgggcact tcagggcgac gtcgccctcg tcggtctgga aggtgtggac cggcagcccc
      841 tggacgaggc cgcccccctc gacgccgcgg atcgcgacgc accagccgta catcgcgaac
      901 gcctcggtga ggcgggtgcc gaaggagtac gccgcgttcc cccagaggta cttgttgtgg
      961 tccttgccgt cgacgtcctc ctcgaagttg aacgtctcgg tcgggaccgt ctccttgccg
     1021 taggggaggc gcatcagcgt gcggggcatc gtgaggccga cgtagcggga gtcttccgag
     1081 tcgcggaagg agcgccactt ggcgtactcg gtccggtcga agatcttcgc gaggtcgcgg
     1141 acctcggtca tttcctggaa gctctcccag ccgaagagct gcggcgccga ggccgcgatg
     1201 aacggggcgt tcgcggcggc cgccacctgg gccatcttct cgagcaggag catgtcctgc
     1261 gggtggttcg agaagtagaa gtcgccgatg agcgcgccga acggctggcc gccgaacgtg
     1321 ccgtactcct cctcgtagac cttcttgaag acggccgact ggtcgaactc cgccgcccgc
     1381 tcgaggtcct tgaggaggtc cttcttcgag acgttcagga cgcggatttt cagcatcacc
     1441 gacgtctcgc tctcgtagac gaggtggtgc aggccgcgcc aggagccctc gagcttctgg
     1501 tacggctcgg agtgcatgat ctcgttgagc tgcgccgaga ggagccggtc gatctcggcg
     1561 atgcgcgcct ggaccgacga ctccaggttg cgcgagagga ccatctggcc ctgcatcgcc
     1621 tgctggacga actccgcgat catgtcgcgg gcctgctgcg tctggctctc gtgctgggcg
     1681 aggcgcccct cgtcgatgat cttcgagagg aggtcgacct ctccttcctg ctcgcgggcg
     1741 atcgcctggg ccttcgcctt ctccgcgccg ctactcattg ccgtccccct tcttcggccc
     1801 ctcgggtgag aggcccagag cgctcgcgag ctgctgctgg ctcccggcgt tctggaggac
     1861 gtcgttcagc atcttctcga gcttctcgtt cccgtccgcc ttcgacagca ggtccgagag
     1921 gcgctgccgc gcctcgacga gctttcggag cggctccacc tgctggacga ccttgtccgg
     1981 ctcgaagtcc tcgaggctct ggaacttcag gtcgaccgcc atcttcgatc cgtcaccggc
     2041 gagcttgttc tcgacggacg tggccacgcg cggggtcatg ccggcgagga cctggtcgaa
     2101 gttgtcgcgg tcgatctcgg tgatcttccg gtccttcatg cgcgggagcg gctgctcggg
     2161 ctggcccgag aagtcgccga ggactccgac gacgaagggg agctccttga gctcgatcgc
     2221 gccaccgatc tcgacgtcgt acgtgatctg gacgcgcggc ggccggaccc gcttgagttt
     2281 ctgctggatg ctttccttct tcaccatgag cgttcctccc tagaagtccg tcggcttctg
     2341 cgttcgcctg ctgtcgcgtc tattcggtcc cgggccggat cccgagagtc tccttgatcc
     2401 cgtccaggac gttcgtgtcc ctgatcacgt ccgcgagcca cgtctcgagc ggcatctgcc
     2461 cccacgagat cgcgcgctgg acgaggtagg agacggggct gtgaggctcg gtctggcgga
     2521 agaactcggc cacctcgccg agccgggcga gcgcctcgcc ccggctgcgg agcgggccgg
     2581 cggggcccgc cgcgcggtac acggctccgg ccgggacgtc gcccgcctcc tcggcgtccg
     2641 ccgcccccgg cgccgacggg tccggctcga gcgcccgctt ctccttgagg atcccgtcga
     2701 cgacgcgccg gacctcctcg agggcgacct tcagctcgcc gaggcccggc gtctgccgtc
     2761 cgaacctctc gtccatgacg cggtcgagag ccaggtactc gtcccacgcg acggcgagcg
     2821 ccttcgacgc ctcctcgtag aagaccttcg gcgtcgacga cttcgccttg cggaagtcct
     2881 cgccgctcgt cttgttctcc tcggccgcct gccgcttcag ctccgcgagg cgcgcctgcg
     2941 cgtcgtggtc gagcgggccg gcggtcgcct cggggacgtc gaacggccgc gactcgctcc
     3001 actcgagcca cgagtagttc atcccgggcg cgcgggtcat cggcgccttg cgcagcgcga
     3061 gcgcgacctg ccggtcgaag aacgagagga cgttcgcccg cccctccatg tcgccctcgt
     3121 cgatctcggg gtagcaggtg tcccagtagt cggcgtggag ccccttcatg aggcggatcc
     3181 cgtcgcgcgc gcccgcgaag ccgtgcagca tcgtgagccc ctcggcgagc caggcggcga
     3241 cctgcaggtc tttcgtcttc gtctggagcg cgtccgtcgc gagcgcgacg gcctcgtccc
     3301 agtcggcgac cttcagctcg cgcttccagt cgccctgctc gagctggtcg tcggcgcggc
     3361 gggcctcccg gatctcgtcg tagagaccgg agtactggag gctctcgccc gacggtcggt
     3421 cgtccgacac cggggcgagg agccgttcga agtcgaggag aggagcctcc gacgacatct
     3481 agatgcctcc caaggattgc gagttggtgt tactgtaacc gactcagcag tccctgtcat
     3541 ctcgtcagcc ccgatccccc gggccggtct ccgcgccggt cggaggcggg gcccaggagg
     3601 ttcgtgatgc cgactcccaa ggagctcctc gactcgggga acctgaaggc cgcgatcgag
     3661 gccctcaccc aggagatccg ggactcgccc gccgacccgc agaagaggac cttcctcttc
     3721 gagctcctcg ccttcgccgg cgagtgggac cgggccgaga aacagatcgt cgcggccgcg
     3781 ggagccgacg cgccgtccca gatggccgcc ggcgtctacc ggggcctcct ccaggctgag
     3841 cgcgagcgcg cccgggtctt cgcggggaag gcccgcccgc acttcctgcg ccccgtcccg
     3901 cccggcgtcg agaagaccct cgccgcgctc ggacggatct ccgaggggga cctcgccgtc
     3961 gcccgcacgc tgctcgacga ggccgaggag gagcgcccgg ccctcgcggg ccgacgcggc
     4021 gagagcgcgt tcgaggacct ccgcgatttc gacgaccgga tcggcggcgt cgtcgagatg
     4081 gtcttccagg ggaagtacac ctggttcccg ctcgagttcg ccaggagcct cgagccgaac
     4141 gccccgaaga agctgcgtga cctcatctgg ccgggagcgc ggatcacggc cgacgacggc
     4201 acgagcgcgg acgtcttcct cttcgccctc tacccgggct ccgccgcgca cgaggacgac
     4261 ctcgtgaagc tggggcgaat gaccgactgg aagaacctct ccgaggacct tcaggccggc
     4321 ctcggccttc gctcgatcct cgtcgacggc gaggacgtga gcctgctcga gctcggcccg
     4381 gtcgagttcg acgagcgcga gccgctcccg agctgagccg gaggcggcgt gcgtcccgtt
     4441 ccggggtgca cgccggcccc cccgccaaga gagagccggc ctcccgcgag ggaggccggc
     4501 tccgtttcga cgatggtcca gagggagaag gctggcctca gcctcccttg ttcgccttga
     4561 ggtcgtagtg cgtcttgatg gcgccgccga gcgtgccgtc cgccttctgc tccttgtact
     4621 cgaactcgat cttggcgaag ttcagggaga tcgagtcgat cggaacgacg tcgctgtcgc
     4681 cgctcccccc cgtctggtag gacgagacga ggaggtcggt gaacgtcagc ttcaggtact
     4741 cctgctggtc cttgccggcc ttgcgcgcgg tcaggatggc cgacttgagg tgcgcaccgt
     4801 tcgcacagtg gagaacgagc ttcggcgtcg ccttgttgat ccgcatcgtg aacgagaagt
     4861 cctgcatctg caccttgccg gcgccaccgc cgccgccgta ctgcatcgac ccggactgcg
     4921 acgcacccca cgcccacgac aggacgtcga tctcgccctt gtgcttctcg tcggcgctct
     4981 cgccctcgat accgtcgatc ttcagaaaat agtcaacgag tccacccatg ttttgcttcc
     5041 tcctttcggc agcttccggc ctataagatg ggtgtcgtcc cgcaggaatt caaggcccgc
     5101 gagccgaccg ggcacatcgg tgtgacctcc cgcaccttca ggtctcccgc ccctcgggcc
     5161 gcagaaacgc ggccagggga cggtcccagc ccttcgggcg gctcggggtc tcgggagact
     5221 cgccgccgtc ggcgggcttc ggcgcgctct ccggcgccgc ggggaccgcc tcctccacga
     5281 actcgtggtc ggacctctcc gggtgcagca ggaggaagtc ctcggcgcgc agctcccgga
     5341 agaacaggac ggcccgggac cggccgcccg accggcagac gaggacgccc gacggagtcc
     5401 tccggccgaa ggccccttcc gtctcgagcc aggccagcca gagctgcatc tgcaccgtcg
     5461 ccggcaggag gttcccgacc gggacgcgga ccgcgcccgc cccgtcgccg gccggggcgt
     5521 cgcaggccgc ccgcaggcga gcgaggaacg acggccacga ctcggccgcg ccctcgccga
     5581 ggagcgactc ggcgaagtcc ccgagcgtga cctcgcgggc ctctttctcg accccctcgg
     5641 cgaccttcgc cggccgcttc accggcacct cgatcttcgc cccgcgatac gcctcgtaga
     5701 acgaggcgag cgtccggccc gccgcgtagg tgcgccggat ctgcgtcgcg cgctcctcga
     5761 agacctcgag gtacgacagc gcgacgagcg ggtcggcgat cgccttcccc tgcgggacga
     5821 cggagaagag cgtgaacggg aagcgacgga ggccgccttc gtcgtggctc ccccagagcg
     5881 cgccggcgac ggaacgccgg cccccgggaa ggggcagaag gaacgtgtgg agcgggatct
     5941 ccgcctcgcg gtactcgtcg tcgttcgccc agcggcggct gaacccgttc ccgagccagt
     6001 cgcggaactc cttcgcgccc tcctcggtca ggccgctcga gatgaagtcc ttgtagatcg
     6061 ggagcttccc gtagagatgc agcgcgcagg tctccatcct cgggggcccg tcggcgcccg
     6121 cgaaaccttc cttcagcttc gagatgagac tccacgctgc cactcggtca cctcgctcct
     6181 tcggaatcgg atcgaaaagg gacggacggg gcccggagcg tccgggcccc gcccgagagc
     6241 ggtcagttcg gcaggatcgg gggcatcggc tcctcgaacg cgagctcgaa gagcccctcg
     6301 agcggctcgg tacgctgcac cgcgttcggg atgacgaccc ggatcgtcca gatcttctcc
     6361 ttctcccgcg cgcgctcggg gtcgtcggag tcgtcgcgcc ggtccctctc ctcgctcgcg
     6421 acgcggacga agtagaagaa gcggagcggc ccgccgtacg cctcgaggct gccggtccag
     6481 ccggagcccg actgctcgtc gcgccccgtg atcgacatcg gggcgtccgc gggcccgagg
     6541 cgccagacgt aggggctcgg ctgccggccc gtcttgaagt cggtcgacgg gcggacgacg
     6601 accgagcggt cgaagaggaa gagggagttg atccggtcgc ccatgaaacg cttgcccggg
     6661 ctcggcgaga actggagagg ccggatctcg accttgtgct ccttgatgac ggacgtcgag
     6721 cgcccgccgc cgaagaggaa gagctgccag ccccgcccga cggcgaggag gccccggccc
     6781 gcgtcgccga cgaagtcgaa ctgcggctcc cagccccaga ggatcgggtt cagggcgtac
     6841 tcgtccgcga gggcgccgac gtcggtcagg atgccggaga agtggttctg gccgatcgtc
     6901 gcgaggtcga gccggtaggt cgcggtgttc ccgctccctc ccggcttcag cgacgagccg
     6961 gccgcgtagg cgaccctctc ctgccggagc tggctctccc tgacgaacgg gaactggccg
     7021 aggcactgct cgcggatctt ccggacgacc ggttcggccg cctgctggaa ggagggctgg
     7081 atcgcgtcgc ggatcaggtt cgcgccgcgg ttctcgacgg cgatcagctg ccgcccgtag
     7141 gagcttcgcc ggacggcggg attccgcgag aacgcgtgga agccgcggag ggagacgccg
     7201 tcctgtccga gcgagagctg ccgccatgcc ttcagctcct gctcggggag cgggacgacg
     7261 ttgttcttga agaggttcgc cgcggtgacg agctccggcg gcgccttcgc cggaccggtc
     7321 gaccgtcgcg gcgcggcctt ccccttgaag tacgggagga tcgaggcgag ccgccggttc
     7381 gaggccgcga acgtctggac gtcgtcgaag gccagcgccc ccgacatgtc ggtcacgggc
     7441 gcgtcgcccc cctgcgactc ggggctccag cgcgtgatcg ccgcctggaa ctcgcgccag
     7501 gacgaggcgc ggagcgccga gcccggcacg atgaagccgc cgcccccgcc gcccccgcgg
     7561 ccggcgaagc ggtcgacgta gcggcggagg tagtcgagca tcgcctcggc gtggccctgg
     7621 gcgagcgcgt cgacgtcggg ccgcgtcgaa atggacgagg cctcggcgaa ggagatgtac
     7681 gagaccccga gcggcaccat ctgctgcgcg tggttgcgcg tgaagcagac gttctcgagg
     7741 caggccggcc cgaccgagcg gtcgaggaac tgctgggtgt acgggaggac gcggtagacg
     7801 agggcgaggt cggccgtccg ggggacgaac gtcgcgagcg ccgtgtcctg ccagagcgtc
     7861 ttctgctccg ccgggatcgc cgggatcgcg gtgaggttcg cgaccgcctt cgccttcgcg
     7921 tcgacctgct tcttcgtgaa cgcctcgagc gtcgccttct ggtcggccgg cgtcgacttg
     7981 cgggccgcct cctcgacgac cgcggcgggg ttctccgtcg cctcgaacgc cgcgaggtcg
     8041 gcgaacgccg tcgcgtcggc caccatgggc gagatcgcgg ccgccgacgg gctccagccc
     8101 ttcttcgccg ggtcctgcgc gtcggggacg acgaggtagg cgtaggcgag gacctggacc
     8161 tcgaggttct tccagtccga gtccatctgc cccttgatcc ggctgcgggc ctgctcgttg
     8221 acgacgcccg gcacgaccgt cgtgaagcgc atcagctggg tgtagtcggc gtcgaagttc
     8281 ctcttccagt cgtcgggacc ggctcccggg aactccttcg acccgggccg gacggtcgcg
     8341 aggtgcttcg tcgtgtcgtc gtagaccctc cggaactcct tgccggtcgc cgcgacgcgg
     8401 tccaggtagc cccgcgagga cgggtcctcg accttgagga gctccgagta gcgctggacg
     8461 tactgggcga ggagctggtt caggcggaag tcccagcgag cgaggttcgc gacgctccag
     8521 tactcctcga acttcttgag cgctcccgcg ggatcgggaa cgcggatcag ctccgggtcc
     8581 ttcggggggt cggaggggag ggccgcgagg atctcgtcgg gagtgccgct cgagccgatc
     8641 gaggcgaccc actcgtcgat ctccttcgtc gcggcggtcc cggccgtccc cttcgtgttc
     8701 tggaggaacg cgagcagcgg gatcagcttc agctccgacg ccgccaccag cggtttctgc
     8761 tcggcgccga gggccccctt cttcacggcc caccacttca tgtgaccgtc cagggcggag
     8821 aagaagacgc ggtagtcgta cgggccggcc cagtcgaggc gggccatccg cgcctcgccc
     8881 tccgccatga gcgggttgac gacgcgctcg taggtcaggc gctggttgat cttcccgagg
     8941 agccggaaga gctcgcccga ggaggcgcgc tggaagaaga gcgcgaagag gaaggggcgc
     9001 tgccggatcg cggcctggtg cttgtagatg ctctcggcga tcccgtaggc ctcgccgatc
     9061 gagcacgcct ccttcttctc gacgcacgcc tgggccttca gcgcggtctc gcgcatcggc
     9121 ttgacgaact tccgcatcgc gacgtacggg aggatcatcc cgaggagaca gaagatcggc
     9181 aggaagaccg tcacggcgcg gatgacccag cgggtcgtct tctcgcgctc gagggccgcc
     9241 ttcgtccgcg cgatgaggcc ctgctcgggg aagaccttct tctcgtagaa gtccttgatg
     9301 aagaacgcgc gcgaacgctt gaagatctgc tcgaggttct cgacgatccc ctccgccgag
     9361 ctcccgagga gctcgcgcgt ggcccgggcg atcggcttgc cctgctggac gccggaggag
     9421 aggtagaacc cgcggaagac gaacgcgtcg tcgtagcgcg tctcctggaa gatcccctgg
     9481 aagtagcgct tcagcgagtt gccgagcgcg cggagctcct cggggaagac gaagatccgg
     9541 tcgacgttct ggacgatctc ctcgttcgcc gtgaacttca tccggagctt gtggacccgg
     9601 gtcaggatct cctcgtacgc ctcgtcgaac gtcgcgagga cgtacggctt gtcggggccg
     9661 accgggttcg accagccgac gagctgccgc tggtcgaccg ggtcgagctt cgagaagaac
     9721 tcggagaagc cgaggatccg gtccgccttc gtgaccatga tgaagatcgg gaagcggatc
     9781 tcgaggaccc gctgcaggtg gaggagcttc gtccggatgt tcgcggcctt cttgtcctgc
     9841 tcctccggcg tgtcctcgag gagcgaggtc gccgggatga ccacgaggac gccgttgatc
     9901 gggcactcct tgcggtactt cttgagcatc ttcaggaagg tcgaccactc ggtggcgtcc
     9961 ggggccgcct cctcctggaa cgtgaagcgg ccggcggtgt cgaggatgac ggcctcgttc
    10021 gcgaaccacc agtcgcagtt gcgcgtcccg ccggagcccg agaggccgtc gccgccgacg
    10081 gggaactcga ggcccgagtt cttgagcgtc gtcgacttgc ccgactgcgg ctcgccgatc
    10141 agcaggtacc acgggagcga gtagacctgc aggccggtcg ccttcagctg ggcgagcgac
    10201 tccgtccact tctgggagag ctccttgacg gcctcgcgaa cctcctgctt ggaggcgccg
    10261 gcgccgccct gcgcgtcgcg cgcgaggtcc ttctcgaacg cgccgccgcg cgccttgtcg
    10321 cccttcttga cgaagacgtc gaacgcggcg atgaggaggg cgatgaggat gagcccgccg
    10381 atgacggccc accagaggtg cccgaagccg agcgcccgcg cgatgacgag gcagacgggc
    10441 gagaggagga cgacgatgcc gagcgtcttg atcggcttgg gaaggcggta gaggaggtcg
    10501 agaaactcgt acatggggcc ctcagatcac tcccatcgcc tcggccacgc tcttgacctg
    10561 gcccaccagc gtggcccagg cgatgaacgt caggatccag aacccgatca ggacgctgaa
    10621 gctgatgatg gcaacgcgcc cgagcgtcac gacggggttc agcctcttcc ccgacgtggc
    10681 catcaccgtg taggcctccg gggtgatctt gtcgctctgg ctcgcgaggt actccgagag
    10741 gagccggtag gtgttcttcc gcacctcggc gagcttctcg ggccgctcgc ggtacttgcc
    10801 gcggaatccg agcgcgagcc cggcgtagac gatcgagccg agctccacgt cctccggccg
    10861 gagctccttc gcgatcgcga agtactggtc gccgccgatg ttcgagcgga agtacttctc
    10921 ctcgaggagg cggtcctgcc agtcgcgcgc gaactcccac ccggagtgga tcaggatctc
    10981 gtccgcgagg acgacgagcg ggtagcgcgc ctggtcgtag agcgcctgca gccgcgggtc
    11041 gctccgcacc gcggaggcct gctcccggaa gatctcgtcg aggtcggcgg ccacgtcctc
    11101 gatgtcggcg cggacgccct tcctcacctt ctgccggaac gacgacagga agaggaactc
    11161 cttcgccgcg accttgaaga gctcggtcgt gtagtccatc tcagctcccg ggcttggtga
    11221 tgatgtagag cgagaacttc agcttcgggt ccagggcgtc ggagagggcc acgttgcggt
    11281 cgcgaacgac gttcgcccag tacggccctt ccttctcgat cttgaagtag aagtagtcct
    11341 ccctttgcgg gaggcctgcc ggcgtccgct tcaggagctc gaggtcgagc ccgaagagcc
    11401 ggcgctggcg caggaccggg atgtcgcgca ccgtgccgag cttcatcgtc acgatccgcc
    11461 cccgcaggtc cttctcctcc atctcggcct cgatcgcgag gaagacctcg gtctgggagg
    11521 cgagccactc ctcgaggagg tccgcgacga ggaggtcctc gcggaggacg aagtcgacct
    11581 tcacgaagcg cgaggcgacg atcttctcga ggagcgcctc gatgacgcgg cacgtctcgt
    11641 ggaagcacgg gccgagcttc tcgtggtcgt agacggggat ccggaccggg accttgccgt
    11701 cgtcgaagat cgagagctcg cccgcgagcc gggcgaactc cgtgtagacg tggaacgggt
    11761 ggatgcgcgg gatcttcgtg agctgctgga agaggaacag gaagctcccg aggatctgga
    11821 gcttgatgat cgcctgccac cccgaggtgc cgtcggaggt gatcgcgaga cgcccctggg
    11881 ccacgtccgt caggagcagg cggtgcttcg cctcgatccg gttcgtcacg ccgtcgcaga
    11941 gcgcctggat cgtccgcgag gcgccgagct cggtcagcga cgggatgaac tcgcgggaga
    12001 ggaccgggaa gttcctcccc gagccggagc gctcgacgac ggcgacctcg aggcactcgt
    12061 agccctcctg gctctcggcg ccgaagaaga gcttgccgtt gcagcgcctc accccgacgg
    12121 gctgcgggtt gccgccggtg ttctcgtcga gggcctcggc gttctcggcc acgtagcgcc
    12181 ggtcgacgcc cgccggcccc tcgcccgggg cgaaggtgtt cggcgcggtc tcgcgcatca
    12241 ccgggacgcc gacgaagacg cgcatcctcc cgctcgcctt gtcgagctcg ttcttgaagc
    12301 tccggggcga gatgcggagg gtcgagtcgg acgacagcgc ggtgccgtcc ttcagcttga
    12361 gggagacgtc gcggatctcg aagacgaagt tctcgagctg gtccggcgcc acgtccagcg
    12421 actggaggcc gaagtagaac ggctggattc gctggatctc gcgctggagc gcctcctcgc
    12481 ggtagcgctc ggccgtctgg aggtgatggg gccggaggaa catcccctcg gcccagagga
    12541 cctgcggcgt cttcttcatg ggaccccgtt accagcggcg ctcgacggcg agctcccgct
    12601 cgaggacgga gacgaggatc ttctgcccca cccacttctc gcgggagagg acgacgacgt
    12661 gtttcgtcgg gtcggggttc tggaacttgt agtcggcgat caggacgagg tagcgctctc
    12721 ccttcgagaa gggaacggag accgtcttgt cgcacggtcc ggccccttcg ccggtcgtga
    12781 aggtgacggt ctggatccgg ttctggccct ggatgttgaa gcgggtctgg tcgtagaacc
    12841 acttctcgcc catctcctgg atcttccggg actcctcctc cgtggcgcgg atgacgtcga
    12901 cggcgagggc gagcccgccg ttgatctggg cgccgcagcg gaacgagatc gtcccgacct
    12961 tcgacgcgca gccggtgagc gcggggaagg ccccgaggag caggccgcag gcggcgatcc
    13021 ggagaaggaa gcgggacggc gtcttcgtca tgagtgttct cctcgctgtg tcttcctgag
    13081 cttctcgagc tcttccttcg aaagtcgggc cacgccctgg aggatctggt cgttgaccag
    13141 gtcgggagag aggtcccgga ccgcgatctt gtagaggtcc caccactcgg ccgcctcgcc
    13201 ccgcagcttc cagccggcgc tccgcggcgc ggactcgatc gtgaccgggt tgatccgctt
    13261 ccagagccgg tcgaaccact cccccggggc ctggtggaag gccgcgagaa tcgcgacctg
    13321 ccacttcttg agctcctcga ggtactcctc cacgcgctgg atgctgaccg gcttgccctg
    13381 ctcgatcgcg tggaggaagt gccggagcgt ctccttgaag ccggggagct tgaaggccat
    13441 cgtctcgttc cccggcatcg tgagggactg gatgaggccg agcgtcatcc cctccatcgc
    13501 gagcgcgtac tcgatcactc ggcggaggac gacgacgttc cggtccgcct gcgcgagctc
    13561 ctcgccgtgg acgagctgct tgagcgggag cggtccggaa gacgccgtcg acgcctgggc
    13621 cttccggcgc tcgacgccct tcgacgggct ctcgtcgacg cgcttcctct cgacctcctc
    13681 gagcttccgg cgaagccggg tcacctcggg gtcgacggcc gcgcccgccg gcaggaccgg
    13741 gaagtgccgc ctgaggagct cgaggaagcg cgtgcgcgac tctctcggga gcggggcgag
    13801 ctccttctcg acgcgcttct tcagctgccg gcgccggtcg tcggaggtgc cgaaccgcag
    13861 ctcccccgcc agggcgaaga gctcgtcggc gagctcgcgc gcccgcccgt cgaggaggat
    13921 ctccgccggc gtccgggggg cgccccaggc gaacggagcg ggcccgggcg ccggcacggc
    13981 cgatgggggc ggcgcgggga gcggctccgg ctcgcgcggc ggctccggcc gcgccgccgg
    14041 agcggcttcg ggctccggct cgtggcgccg ggcgggagcc ggggcgaccg cggacgactc
    14101 cggggaggcc gggggaggag gcggaggagg gggcggaagc gtccgcgaaa gcggggacgg
    14161 cccgcccttc gccgtcgcct caccgacctc ctccgtcagg agcgtcgtcg cgcggccttt
    14221 ccccgccgtc cccttcatcg aggcccgccc cgtcagctcc cgctccgcgg gtcgtgcggc
    14281 cggggccttc gccccggact ccccgacgaa tccctccggc acggaggtga tcccgcaggc
    14341 ggtcagccag tcgcccaccg gtccgaacct gtcggcgacg aggccggcga ggacgctccg
    14401 ggcggcccag cggaccgcct cgtcgccgga gaccccgtcg gggaccgccg tgcgggtgcc
    14461 gtccaggagg cgcgcgacgc ggggagagac gaccgcggtc tcgaagctcc cggtgaggtt
    14521 caccggcttg acgaggatct ccgcgatcca gccgagcgtg tcgcggaaga agggtttgtg
    14581 aaagagagcc tgacggacct cggccggccg cacgcccccg ctcgcgagga gctcctcgat
    14641 ctccgggtcg ccgagggcga ggatcgcgtc gagcaggtgg cgcgtgtcga cgtacccttc
    14701 ccgccggccg gccgcgcatt cggccgccgc cgtaaggagc ggctcgacgg acgccccctc
    14761 ccagtgcgtc acggcgagcg gctcgaacgg cggcggcgcc gcgagcgggc tcgtcaggta
    14821 gacctggacc ggcgtgagcg agacgaggaa gatctcgccc gacttcacct cggtctcggt
    14881 cgtcaccgcc tgctgggcgg cgaacgtccc gttggagctg ccgaggtccc tgaggaagat
    14941 ccgcccctgg gcgaagcgga cctccgcgtg ggagccggag acgccggccg acacggagag
    15001 gacgaggtgg ttgcgctcct tcgtctgccg gtcgaacggg aggcgcccga tccgcacgag
    15061 ctgcatcgct tgccgcgctt cggcggcgta gcggagcccc tccatcgggc cgagccagac
    15121 ctcgagcgtg agagagcgcg gcgggccgct cacgcgcacc ccccggagaa ggggcgacac
    15181 gccagcacct cgaacgagga gacggacgac ctgtcggaca cagtggccgg atcttaccct
    15241 cggtccgcaa accctgcgac ccggtccggc gtcaggcgcg cggcgccgtc tccgattccg
    15301 cctcgagcag ctccgcgagg acgtcctgga tctcgaagtg gagcggctgg cgatggacga
    15361 ggagcgcccg gagctcggcg acgacctcgt ccgtctcccg gagcacctgc tccacgcgtc
    15421 ccgtcgggtc ctccgccacg aagtccccgg gacccgtcgc gatcgagaat cccgggatcc
    15481 gcgtcaggag gcggagcgcc aggaggagcg ccctctcgaa caggggcggc gggatcgcgt
    15541 tcggccggcc ggggacccgg tcctcctcct tccagaagac gcgggagcgg tcgaggagcg
    15601 tcgcggtcga agcctcggcc gcgagggccg ccttcacacg cgcgagcgcg tccccttcct
    15661 tcgaccgggc cgccgtcgcg gcggcccgct ggaccccctg gagcagctcc ggcagctcgg
    15721 tccgctcgcc gccggcgagg gagcgcagga ggaggacgcc gagggagtgg aggtcggacg
    15781 gcgcgccgaa gcgcgggtag accttgtagc gcgcgccggg gaggcggaca ccgaaggagc
    15841 gtcgcagcct cttcaccgcg gcgtcgtcga gctcgaccgg ctcggagacg aacgtgagct
    15901 cctcgtacga ggccgggacg tcgggcttgc gcctcacgac gagagcgtcg atgccgaggc
    15961 cgagcgcttc gtcggggaac gtgacgtgga tccagtcggg ctcctccggc cgcgggaaga
    16021 gtccgttcgg gtcgctgagc cgcccttcga ccttcacgcc gagccccgcg tccgccggct
    16081 cgatctcgga gaggtagacg tcgccgggac gccgcgaggc gaggcggaac tcgaggatct
    16141 cgggcgccgc gtaggggaac atcggctccg gcggcggcac gacgacctcg acgccgaagg
    16201 acgacgtccg cgaggccgcg gagagcccgt gcagccgcac ctggaacgtc cagagagccg
    16261 gaagcgaggc gccggcgccg tagctgtcga ggaggacgtg ccccgggtgc aggtcgaggt
    16321 gcggctgtcc cgtggcgcgg tagaacgcga ggaggcccgc ggcgagctgc cggaaggccg
    16381 tcatcttcag gtagaggacc tcgatcgcat cgactcccga gccgtcgtgc ccgtagagga
    16441 aacgccccgt cggcgggagc gacgcccgca gccattcgat ccggcggcgg gtcgattccg
    16501 tctcgagggt ctcgggcgcg gccggcgaga cgaggtcctc ctccgtccgt ccgccgacga
    16561 agtcggccca ctcgtcccag cgcaccgggg agagcccgct cacgaggaac ggagaggggc
    16621 agaacgagaa ggggctccag cgcgcggcga agtcgttgag cgagccgccg ccggccgcgc
    16681 ggagacggag cgcctcctcg gccgactccc gggaggcgaa cgccttcacc tggccttcgt
    16741 cccagtcccg gagcagcgcc cgggagagcc ctttcagcag ctcgtcgggc ccgcccaccg
    16801 ggacgtcggc ctcgcccggg aggggcgaat aggagaagac ggcgacgacg cggtccgtgg
    16861 tggcgcagcc cgcgcagccg acgagcctgt ggagagattc caggtacgac ggaagccgcc
    16921 tctcgcgcag gaactgctcg tcggtgagga gctcgagcgg gcggccgcac gaggcgcacg
    16981 gcgggatgaa gaacgtccgc gacggcttgt cgtagacgag ggccgggagc gtcccctcgg
    17041 gaccgccgtc cggctgcagg agcctcggga agtacgggct ctccgcccgg agcgcgcgca
    17101 tccgctcccg ctccgcgtcg aagcgcgcca cgagggtccg gttcgtcggt cgcgcggcgc
    17161 tctcggcgag agcgtccggg aggacgttcg ggaggagctt gacggcgacg agctcgacga
    17221 cgctgtcggc gtctcccttg agggctccga ggtagacgcg gctcgtcccc tcgtccgccg
    17281 agagcggcag ggggaccggc ccttcctcgg ccccgcccgc gagcggtccg aggacgaggt
    17341 ggagcccgaa gggcgacggg gcgtcgcccg gggggaggag gcgggcggtg ggaaggccct
    17401 tcaccgggac cctcccgccc gccgcgccgt acggacgttc ggcacgcgcg cgctcaccac
    17461 tgaaattcct ccggagaccc gagtctaccc ttccccgccc ccggcctcgc tcccggccga
    17521 ccccgtcggt cgccgcagga ggcgggcgag gaggacgagg agcgcgacga cggccccgac
    17581 gagcgcgacc cagaagaccc tcgacccggc ggagggcgcc acggcgggga gcgcctccgg
    17641 cgccagctcc acctccgtcg ccggggcgcc gaggagccgc ggggccagga gctcgaggtc
    17701 gtagcggggc acggcgaggc ccggccggcc ggcgaggagc gcgagagggc cgtcctcgcg
    17761 gacgaagcgg agccggtgcg ccgggaggag cagcgccgcg cgcgagagcg ggagtggagc
    17821 gttgtcgccg tcgtcgatcg agacgaagag cgtccccccg gttcccggcg gcagctcgag
    17881 gacgaggcgc ggcgcctcgc gcgccgggtc ggcgctctgc cacgaagacg aggtgatcgc
    17941 ggtgaacgtc ccggccgtct cgggccggcc gccgaggcgc tcgcggagga ggacgacgct
    18001 tcgcgcgaag acccgcgccg tcgtcgtgag ctcgaggcgt gaggcgggga ggtcctcgtg
    18061 cgggagctcg atcctgtgga acgagagcct cccgccggtc ccctctccac cgcggggacg
    18121 cgggtcgtcc acccgctgcg gggcggggag ccggacgacg agcggctccc cgagcgtctc
    18181 gaggagatac ggcacctgcc gcccgtcggc ggaagcgatc cggacgtcgg cgaggctcgg
    18241 gctcgccgcg agcaccgccg cgtccagccg gagcgccgtc aggccgggcg ggccgggcgg
    18301 gatcgggcga gagacgcgga acgcgtcgcg ctcgagcgcc gcgcccgccg ggacggccgg
    18361 cggcgcggac gcttcggcaa cgagggccgg acgcggccgc tcctctcccc aggacgcgct
    18421 cgccggcggc ggggcgccgc gcctcgtcac ggcgggccgg gcggcctcga ggtcgtacct
    18481 cggccgttcg agccccggag ccccgtaccg ggccgcgagg ggcgcgccgt ccgggctttc
    18541 gaagtagacg aaggggagcc cttcgaagag ggccgtgacc gaagcgagct cgaggggcgg
    18601 gttccccgcg tcctcgacga cgagctccag ctccgcctcc gtcgggggag tgagggtgat
    18661 cgtcatctcg gccgctgcgg cgtcctcgcg ctcggccctc cgaagcgtcg cggcgcccag
    18721 ctcgtacggg acgaggcgcc cgtccgcgag tcgcgactcg aggacgcgcg cctcgcgcag
    18781 gacgttcccg gatcggacac cgacctcgag agccgacagc gggagccccg cggccggaag
    18841 ccgcagccgg taacggctcc gccccggctc gctctcccgc ctctcgaacg agacctccgc
    18901 ccgaagcgtc tccggcgtgc cgccggtccc ggccgcgcgc gcctcgacgc gggacggggt
    18961 cgggacccgg ccgctcttcg cgtcgttcca gacgagccgg aagaaccggt gctcccccgc
    19021 ggggaacgcg acgaccgtcc gcgcgaggcc ctcttcgggc aggtcgaaga gcgtcccctc
    19081 cgcgacgaga acggcccagc gcgcccggtc gccgctcccc tcgagacgga gacgcttcag
    19141 gaacggcgcc gggatcccgt ggacgcgaag gcggtcgacg caacggagcg ccccgaggtc
    19201 ggcctcgaac ccgctcgccg tcttcgtcgc ggcgatcgga aggagcgtgg cggggagcca
    19261 ctccggctcg gatcggcctg gagaaatgag caggtagggc acctcgcgtc cggcggcgtc
    19321 gtggaatcgg aggtccgaga gcagaccgct ccgggagcgc gcctcggagc cggcgaggag
    19381 cgggacgtcc acgtcgaggc gattcggtcc gggcgcgccc ggaaggacgg cccgctcgtg
    19441 ccgcggggac gacacgggac ggactccttc ggccgccgcg ccggggaggg cgacggcgag
    19501 gagaccggag gcgaggatcc ggcggatcgg cgtcattcct tctcccgcgg gcggaggacg
    19561 aacttctgga tggcgatcgc gacgagcgag agcgagatcg ccagaccgac gaacgacgcg
    19621 acgcggtaga gcccgccgag ccgcccgagg tcgtgcagga acgccttgag gaccgtcacg
    19681 acgaggaggc cgagcgccgc ggcgcgcgcg gcccggctct tcccgacgat gccggccgcg
    19741 aggaggccga cgccgaagac ggcccaggcg agcgtgtacg cgaggtcctg cgcgagcgac
    19801 gtcccgcgcg cgaggt
//
LOCUS       DAOONL010000064        19779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2744_length_19779_cov_17.219580, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000064 DAOONL010000000
VERSION     DAOONL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_19779_cov_17.219580"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLP50_10580"
     CDS             <1..992
                     /locus_tag="PLP50_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HPA52034.1"
                     /translation="AADEAAVVAAASAFVRDLGNTPGSDMVPRTFAEAVKAAARKRGV
                     KATVLGKKEIERERMGGLLAVNRGAAEEPRFVILEHRGGRKGEKPVVLVGKGVTFDSG
                     GISLKPADRMGDMKWDMLGAATVCGAVLAAADLGLPVNVVALATLTENLPSGTAYKPG
                     DIVTFRNGKTAEIDNTDAEGRVILADALDYAKELAPAAIVDFATLTGAALVALGLEAS
                     IVFSDHDDLVKELVSAGERTDERLWRLPLWDDYKENIRSDWADFKNTGGRSGGSINGA
                     MFLKEFVDPKTPWAHVDIAPNAYFEREHAGYAPGATSIGLAMTLRWLRDRAAR"
     gene            997..2769
                     /gene="recN"
                     /locus_tag="PLP50_10585"
     CDS             997..2769
                     /gene="recN"
                     /locus_tag="PLP50_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPA52035.1"
                     /translation="MLSSLRVRDLAVLEDVDVALEPGLTVLTGETGAGKSLVVDALSL
                     LSGERSDATLVRGGAERLVVEGSFETDDPAVGAALSAAGLRDGDARGPSELVVRREVS
                     SAGKGRLFLDGSPAALRTLAEVAPRLLVLYGQAEARELLDPAAPRELLDRFAALAERA
                     AEVARLHAAFREKEADRERIAALGKDGPARLEVLDFRIGEIDRAAPVAGEEEALAREK
                     ASLQNVEKRGRLLADAVAALEGDEGGALPALTALRRTFAALAEIDASAAEKLAAADEL
                     VERLSDLAASTSRELERLEADPARLAEVVERLDLVARLKRKYGATLEEVLSYRDAIGR
                     ERDELADLDGALRKAAQASEVAFAAFRAASLELSSARQAAAPKLAKAVEKHLSDLAFL
                     SSTFQVEVSRRAEPDGAFLAGEERVAFGPHGIDVVSLAFAPNPGEPPRPLAKIASGGE
                     LSRVQLAVAAALAEAETAREGRRGSRRGGPVRTFVFDEVDAGVSGATAEAVGRKLRAL
                     AGRDQVLVVTHLPQVAAAGERHLSVRKEVAAGRTRTVVTSLDEKGRVEAIAALLAGAS
                     VTDAARRQARALLASAGDDARPAR"
     gene            3096..3653
                     /locus_tag="PLP50_10590"
     CDS             3096..3653
                     /locus_tag="PLP50_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPA52036.1"
                     /translation="MIIRPFRGATPVLGERVFLAETAAVVGDVVLGDDVSIWYGSVVR
                     GDCCTIRIGARSNVQDNSTLHVTKDVGPLLLEEEVTLGHNCVVHACTIRRGALVGMNA
                     TVLDGAEIGERALVAAGAVVLGGTVVPPRTLWAGSPARLRRPLTDAEAEGLDAYWRNY
                     LEYKEEYLREDVRRGRSLPLPVPVL"
     gene            3670..4929
                     /gene="hisS"
                     /locus_tag="PLP50_10595"
     CDS             3670..4929
                     /gene="hisS"
                     /locus_tag="PLP50_10595"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPA52037.1"
                     /translation="MITSVKGMRDLLPPDTRVWNRLEETARTVFSLYGYDEIRTPVLE
                     PTELFVRSVGEASDIVHKEMYAFTDRGGRDVSLRPENTAGVARAYVEHQMPQAGGVRK
                     LYYVGPQYRYERPQKGRYREFRQIGAEVLGAADPATDAELLVMLFDLFTRLGFTGLSV
                     SLNSVGSEESRPAYVEALRAYLAAHEGTMGEDDRRRLRENPLRVLDTKDPALAAALAG
                     APRVLDHLDPASKAHHEELLALLEASGIPYRVEPRLVRGLDYYTRTVFEVTAQGLGAQ
                     DALLGGGRYDRLVSDLGGPRTPGIGFAIGEDRLVDVLPEPFRRAAIDAFAPVAVMAID
                     PPDRAAALALAGELRRAGVAVDLDPAGKGPGHALKNAAKRGCRTAVLVGEKERQSGVV
                     VVKDLVERSQREVPIGDLPRTLKGERP"
     gene            4935..6707
                     /gene="aspS"
                     /locus_tag="PLP50_10600"
     CDS             4935..6707
                     /gene="aspS"
                     /locus_tag="PLP50_10600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPA52038.1"
                     /translation="MVARVPAGSLRREDAGRTVQVKGWVHRRRDFGELVFVDLRDRSG
                     ILQVVFDAELLDEPELLSRAKELRGEYVLAVTGAIVPREGSARNPDMPTGDVELRATA
                     LELLNRAETPPFVIEDDVRASEDLRLKYRYLDLRRPEMTSNLVKRSDVTFRIRQVLHE
                     EGFLEVETPILTKSTPEGARDFLVPSRVHPGEFYALPQSPQLFKQLLMVAGMDRYYQI
                     ARCFRDEDLRADRQPEFTQVDLELSFPDEETVYALIEKLFAAVFPAYGIPCPVPFPRI
                     THAEAMDRFGIDRPDTRFGLELRALDVSASPSEILRGAETVKGIAVPGGAAFSRKRLD
                     ELEAAAKQLGAAGLVWVKLQAEPPGSWGSNAKKMLDDATVASARAALDAKDGDLLLVV
                     AGKRTTVNDVLGALRLRVAREEKLVPEDRFDFLWVTDFPLLEWHPEDGRWYATHHPFT
                     APRPEDVPLLDTDPGKVHARAYDVVLNGMELGGGSIRIHQPEVQSKVFATLGIGPEEA
                     KAKFGFLLEAFRYGAPPHGGLALGLDRMLMVLTRSASIRDVIAFPKTASASCLMTESP
                     GPVADAQLAELGIRLAGDDGPKPA"
     gene            6685..7797
                     /locus_tag="PLP50_10605"
     CDS             6685..7797
                     /locus_tag="PLP50_10605"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPA52039.1"
                     /translation="MTGRSRPEPRAGRAFRFAAAGVPCEVAVGEGALARLADALPRIA
                     PGRWLVVSSAPVLAAQGDRLRTALARARSVDPEPLVVPDGERAKSWAELGRLLSGMAA
                     RGLARDGGIVAFGGGTVGDVCGLAASLALRGVPVVQVPTTLLAAVDSALGGKTAVNLA
                     GGKNLAGSFHHPRFVCADTRLLDTLSERDHRSGLAEIAKSALLSAPFWRRLPRLVEGL
                     VSRDPLALAEAIGRSLRLKASIVSRDPDEALGLRHVLNLGHTIAHALEGASAFRLRHG
                     EAVAWGLLAALRLSSVRGLLTSARAEAAASTLRGLLAPPPLDARVRRGFPELLSRDKK
                     RDRAGLRDVLLEAPGRPRLVRVTVAELAASLPSGDG"
     gene            7807..9558
                     /locus_tag="PLP50_10610"
     CDS             7807..9558
                     /locus_tag="PLP50_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPA52040.1"
                     /translation="MAALPRRPRLRIVTALIATLVVVSIVPLALSAVRLVSINRDALE
                     TAEAQYLTRSAVTLSDAIDAYLRRMLVQVKKIGDGLELARAVAPGEDPFAFIGRTGLI
                     GRYRDAEPSFLVLRALDPDRRGGVVQPAGLDPSLEKELDRAFDTALRGSVYVGDALRI
                     PGLPDPGLVMAAPVPAPDGETVGVVEAFVSLAPIRALLEQERKRDLVAYVVDRRGSLV
                     LSSDPAIFGGKEMLLDVELVAEFVQHPVRLTRTYPQGEGETVRRVLGTVAPVGPAEDL
                     PDWGVLVEKDVARAFAAATQMTRTSWLVAALAVALAVVAAILAANALSRPVRALAESV
                     HRMSEGDLSQRVDVPGTYELAELAEGFNHMGGELEKSIEKLKLAARENQELFLNSIRA
                     LAAAVDAKDPYTRGHSERVARYSVAIARHLGQPLEEVRKVRIAALLHDVGKIGIDDRI
                     LRKPTALTDDEFEIMKLHPVKGALIMGQIPQLKWIIPGMKYHHEKWDGTGYPDGLRGE
                     EIPMLARIISVADTFDAMTTTRPYQKAMRSDYVVSRIKTFAGTRYDTAVIEALEKALQ
                     KNDLEVIGEAARMAEAS"
     gene            9555..10349
                     /locus_tag="PLP50_10615"
     CDS             9555..10349
                     /locus_tag="PLP50_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52041.1"
                     /translation="MRGVCAVTAVVALWVLSGCQSTLDAAKKLPEPSPAVADLVDYRQ
                     GLALLREGRVDEAIQQLGSARAAYPRSAEVANALGLAYLYRKDHRNATKLFAEALELD
                     ENLVEAKNNRGVAAMEAGRFEDAEKDFGDVLSGSRTSEHVKARYNLGLLRARQLRWAD
                     ADRELTTVLADDPGYVDAARERGLARMKLEDFRGALEDFLLYLRENGNDPVANYNAAL
                     CLLTTDRRDLAVRYMERTVQSAPESDEARKARRFLGGEAGAAGRER"
     gene            10346..10696
                     /locus_tag="PLP50_10620"
     CDS             10346..10696
                     /locus_tag="PLP50_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52042.1"
                     /translation="MSFEDVLRPVLDAPGAQAVAFLDPQGEAIASLGDHVLLETLGAY
                     QSVWTTELARAAMGGGLGEVTEVDFEFDAGRVLATAVREGYFLLALFGRDGVPSVARP
                     SIAAARTRLAAEIG"
     gene            10711..11646
                     /gene="ftsY"
                     /locus_tag="PLP50_10625"
     CDS             10711..11646
                     /gene="ftsY"
                     /locus_tag="PLP50_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPA52043.1"
                     /translation="MAEGARTSLMARLKRGLFMTHTELIARVGDAIKARFSPDPKALE
                     ALEEALLAADVGPATASELVEAVRVEAGKRDAGEADVVRRVLKAEIERRLSVEGPASA
                     LVPPGMPRVVLMVGVNGTGKTTTAAKLAARAAAAGGRPVLAAADTFRAAAIDQLEVWA
                     GRIGIPLVKHRPGADPAAVVFDACAAAKARGADLLLVDTAGRLHTKHNLMEELSKIRR
                     IAGREIPGAPHEVLLVLDAVTGMNGLAQAREFLKAAGVTGLVLTKMDGTAKGGVILAI
                     VRELNLPVRYVGVGETADDLLDFDPAGFAAALIDD"
     gene            11639..12757
                     /gene="ribD"
                     /locus_tag="PLP50_10630"
     CDS             11639..12757
                     /gene="ribD"
                     /locus_tag="PLP50_10630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPA52044.1"
                     /translation="MTDLASAFERVFELAERGRYSTSPNPRVGAVVVDAAGAVVGEGW
                     HERAGSPHAEAVALAEAGSRARGATVVVNLEPCAHHGRTPPCADALVAAGVARVVFSV
                     LDPDPRTAGKGRERLREGGVATETGAFAERAERLNEPFLTAARSGRPFVHLKWGASLD
                     GKIATASGRSRWVTCEEARADGMRLREECDAILVGAGTVRTDDPALTRRAGLNRSVVP
                     HRRVVVDGRLRVPADRKVFSPAAPGEAWLATAVPEADPRLAPFRDAGVRVLSLPSAAP
                     GRVDLAALLVRLAGSDVRSLLLEGGGETAFSFLESGLVDRVTGYYAPLLLGGREAPSA
                     LGGAGFPTLEGACRLSHLEVAPLGDGFRVSGRVARPAA"
     gene            12767..13411
                     /locus_tag="PLP50_10635"
     CDS             12767..13411
                     /locus_tag="PLP50_10635"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPA52045.1"
                     /translation="MFTGLVEAVVEVRAVGRRAGGALLDVERPATFGDVARGESISVS
                     GVCLTAVPGVEAGRLRFDVSAETLERSTLGALRPGDAVNLERALAVGGRLGGHVVQGH
                     VDATAPVTRLERSGEFWTLGVRLDPSFARYVVEKGSVALDGVSLTVASLAEEELTVAV
                     IPETFRATTLGRRRAGALLNVEVDVLAKYVERLLGRAAAPSSTMDDRLRALLGS"
     gene            13408..14532
                     /gene="hemW"
                     /locus_tag="PLP50_10640"
     CDS             13408..14532
                     /gene="hemW"
                     /locus_tag="PLP50_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPA52046.1"
                     /translation="MTARPGAYVHVPYCAHRCAYCSFVAVTGRETERDYFDAVVAEIE
                     GRAGEVAGPLETVYFGGGTPSFADPESLLRVLHTLRRTWGLVPRAEVTAEANPDDLVP
                     ERLDALAGLGVNRLSVGVQSLRDAELVPLERRHDAASARDAVRRALGRGLRVSADLMI
                     GIPGQTREGLLEGLSELLAAGVGHVSVYLLEIEKAPRLVALRESAPALFAGDDEMAER
                     WERVDDACHAAGLPRYETSNWARPGEESRHNLKYWRREPVVAVGVSAASFDGLVRRTN
                     GGSIPAYMAAIREKGTAFVSEERLPADAALREEVLLGLRTVDGVEARTFEAALGTLPA
                     AERERLADAVAAGLLETAAGRVRLTRRGVLLSNEVFALLL"
     gene            14668..16887
                     /locus_tag="PLP50_10645"
     CDS             14668..16887
                     /locus_tag="PLP50_10645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPA52047.1"
                     /translation="MNAETLVYVIPVFGLAGLLYTWLKSSWVARQDVGTERMAKIAGA
                     IQQGAMAFLRAEYRVLGAFVVVVALLLGVSGALQDGSSPLVAAAFVAGALCSALAGLI
                     GMKVATKANVRTTQAARRGLGPALEIAFAGGSVMGLGVVGLGVLGLGGCFLVFSKLFA
                     GDVHKTIAVITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHPLNPA
                     TIADNVGDNVGDVAGMGADLFESYVGSIVGAMVLGAAFMTGRLATGEPFTTDGFGGLS
                     AVLLPLVLAGVGILTSIAGTFFVRVREGGSPQRALNTGEFGSSIVMVVLSFFIIRGML
                     PGSFVFDDALYGAERTITSLGVFFSTVIGLAAGLAIGVLTEHYTGTGTGPVLSIARQS
                     VTGAATNIIAGLGVGMRSTAWPILILAASIIGAFHFGGLYGIAIAAVGMLSNTGIQLA
                     VDAYGPISDNAGGIAEMAELPKDVRSRTDKLDAVGNTTAAIGKGFAIGSAALTALALF
                     GAFMTAAHLKTIDVSKAEVMAGLLVGAMLPFLFSSMAMAAVGRAAMAMIQEVRRQFTE
                     IPALKSAIFAMDAHSGKDFKDWPAEDQKIFEDALGKAEYGKCVEISTTAAIKEMVAPG
                     LLAVIVPVVVGFGPRLLGLGSGAEMLGGLLAGVTATGVLLAIFQSNSGGAWDNAKKMF
                     EEGVEIDGHRYYKGSDPHKAAVVGDTVGDPFKDTSGPSLNILLKLMSVIALVIATLL"
     gene            complement(17223..17669)
                     /locus_tag="PLP50_10650"
     CDS             complement(17223..17669)
                     /locus_tag="PLP50_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPA52048.1"
                     /translation="MGMDIGGSKGGPKSEINVTPLVDVMLVLLIIFMILQPMLQMGYD
                     VNVPPNTPSNVLPQANPEQIIVSLTANNEIYLNKERVERSNLPIRLQEVLRNRGKKPV
                     FFSCEDTVKYDDAMRLMDVVRNNGAENIGIVMDWVNPLEQVAPAGS"
     gene            complement(17681..18094)
                     /locus_tag="PLP50_10655"
     CDS             complement(17681..18094)
                     /locus_tag="PLP50_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HPA52049.1"
                     /translation="MEDFGGRHETKSEINVTPLVDVMLVLLIIFMVVTPMLQKGKPVL
                     LPQTDQPDKKPESDKELLISVQSDKQIFIDAKWYPDQEFAAKMREFGERAASKDVLIK
                     ADKSLQYGDVRKVMRMIKDGGFEKIGLITERKAEK"
     gene            complement(18161..18841)
                     /locus_tag="PLP50_10660"
     CDS             complement(18161..18841)
                     /locus_tag="PLP50_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPA52050.1"
                     /translation="MEMDLGHIWAQMSITVKAIMVLLVFLSVYSLGVSLERWWAFRNA
                     KKQSVKFALEIGPLLKQEKLKEAIDLAKKYKASHVAKVVSPGLLEFAYEAHGGNVAGH
                     DVVAAAERAITRAAMMTGADMRRGLGGLATIATTAPFIGLLATVLGIIRSFQGMATSG
                     TGGLGAVSAGIAEALIGTALGLFVAIPAVWLYNYFLNKIERFNVEMSNSSSELLDYFI
                     KRMGSRAA"
     gene            complement(18899..19639)
                     /locus_tag="PLP50_10665"
     CDS             complement(18899..19639)
                     /locus_tag="PLP50_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPA52051.1"
                     /translation="MFETSLIESKKQKPTGSRWLSVPMSVFLHFVVGGTVLAASMWYI
                     EDIPEPPIPVTFYTEAAPPPPPPPPPPKAAAPKAAPKVEAVKPSANAAPTIIPDVLPV
                     APAAPEADDSGIEGGVEGGVEGGVEGGVMGGVLGGVKGGVLGGAAEVEEPLRVGGDVK
                     EPVEISRVQPVYPEAARKARLQGIVILEAIITKTGSVESVRVLRGINPLLDNAAMRAV
                     QQWKYKPATFNGRPVPVYLTVTVTFKLQ"
BASE COUNT         2658 a         7024 c         7239 g         2858 t
ORIGIN      
        1 cggcggccga cgaggccgcg gtcgtggccg cggcgtcggc cttcgtccgc gacctcggga
       61 acacaccggg cagcgacatg gtcccgcgga cgttcgccga ggccgtgaag gccgctgcgc
      121 ggaagcgggg cgtgaaggcg accgtcctcg ggaagaagga gatcgagcgc gagcggatgg
      181 gcggcctcct cgccgtcaac cgcggcgccg cggaggagcc gcggttcgtg atcctcgagc
      241 accgcggcgg gaggaagggg gagaagcccg tcgtcctcgt cggcaagggg gtcacgttcg
      301 actcgggcgg catctcgctc aagcccgcgg accggatggg cgacatgaag tgggacatgc
      361 tcggcgcggc gacggtctgc ggggcggtcc tcgcggcggc cgatctcggg ctccccgtga
      421 acgtcgtcgc cctcgcgacg ctcaccgaga acctgccctc ggggacggcc tacaagccgg
      481 gcgacatcgt caccttccgg aacgggaaga cggcggagat cgacaacacc gacgccgagg
      541 ggcgcgtgat cctcgcggac gccctcgact acgcgaagga gctcgcgccg gcggcgatcg
      601 tcgacttcgc cacgctcacg ggcgcggccc tggtcgccct cgggctcgag gcctcgatcg
      661 tcttcagcga ccacgacgac ctcgtgaagg agctcgtctc ggccggcgag cggaccgacg
      721 agcgcctctg gcgcctcccg ctctgggacg actacaagga gaacatccgc tccgactggg
      781 ccgacttcaa gaacacgggc gggcgcagcg gcgggtcgat caacggggcg atgttcctga
      841 aggagttcgt cgacccgaag acgccctggg cgcacgtcga catcgcgccg aacgcctact
      901 tcgagcggga gcacgccggc tacgcgccgg gcgccacgtc gatcggcctc gcgatgacgc
      961 ttcgctggct ccgggaccgc gccgcccgct gaggccgtgc tctcgtcgct ccgcgtccgc
     1021 gacctggccg tcctcgagga cgtcgacgtc gcgctcgagc cgggcctgac cgtcctgacg
     1081 ggggagaccg gcgcgggcaa gtcgctcgtc gtcgacgcgc tctcgctcct ctcgggcgag
     1141 cgctcggacg cgacgctcgt ccgcggcggg gcggagcggc tcgtcgtgga aggctccttc
     1201 gagaccgacg acccggcggt cggcgcggcg ctctccgccg cggggctccg ggacggggac
     1261 gcgcgcgggc cttcggagct cgtcgtgcgc cgcgaggtct cctcggcggg gaagggacgc
     1321 ctcttcctcg acggttcgcc cgcggcgctc cgcacgctcg cggaggtggc gccgcggctc
     1381 ctcgtcctct acggtcaggc ggaggcgcgg gagctcctcg accccgccgc gccgcgggag
     1441 ctcctcgatc gcttcgcggc gctcgcggag agggccgcgg aggtggcgcg gctccacgcc
     1501 gcgttccggg agaaggaggc cgaccgtgag cggatcgccg cgctcggcaa ggacgggccg
     1561 gcccgcctcg aggtcctcga cttccggatc ggcgagatcg accgggcggc gcccgtcgcg
     1621 ggcgaggagg aggcgctcgc gcgggagaag gcctcgctcc agaacgtgga gaagcgcggg
     1681 cgtctcctcg ccgacgccgt cgcggcgctg gagggggacg agggaggcgc gctccccgcg
     1741 ctgacggccc tccgccggac gttcgccgcg ctcgcggaga tcgacgcctc cgcggcggag
     1801 aagctggcgg cggccgacga actcgtcgag cgcctgtccg acctcgcggc gtcgacctcg
     1861 cgggagctcg agcgcctcga ggccgacccc gcgaggctcg ccgaggtcgt ggaaaggctc
     1921 gacctcgtcg cgcggctgaa gcggaagtac ggagcgacgc tcgaggaggt cctttcctac
     1981 cgcgacgcga tcggacggga gcgggacgag ctcgccgacc tcgacggcgc gctccggaag
     2041 gcggcgcagg cttcggaggt ggcgttcgcc gcgttccgcg ccgcttcgct cgagctctcc
     2101 tccgcgcggc aggccgcggc gccgaagctc gcgaaggcgg tcgagaaaca cctctccgac
     2161 ctcgccttcc tctcctcgac gttccaggtc gaggtctcgc gccgcgccga gccggacggg
     2221 gcgttcctcg ccggcgagga gcgcgtcgcg ttcggccccc acggcatcga cgtcgtttcg
     2281 ctcgccttcg cgccgaaccc cggagagccg ccgaggccgc tcgcgaagat cgcctccggc
     2341 ggcgagctct cccgcgtgca gctcgcggtc gcggccgcgc tcgcggaggc cgagaccgcg
     2401 cgcgagggtc ggcggggcag ccggcgcggc ggccccgtga ggacgttcgt cttcgacgag
     2461 gtcgacgcgg gcgtctcggg agcgacggcc gaggcggtcg ggaggaagct ccgggctctc
     2521 gccggccggg accaggtcct cgtcgtcacg caccttccgc aggtcgcggc cgcgggggag
     2581 cgccacctct ccgtgcggaa ggaggttgcg gcggggcgga cgcgcaccgt cgtcacgagc
     2641 ctcgacgaga aggggcgcgt cgaggcgatc gccgcgctcc tcgcgggagc ctccgtgacc
     2701 gacgcggcgc ggcgccaggc gcgcgcgctc ctggcgtccg cgggcgacga cgcgcgcccg
     2761 gcgcgctgag cgaagcgcgc gcgctctgtc aaggacgaac gcgaggtcct ctcctctgca
     2821 ggcttcgtgc ccgtcccgct tcgagacacc acgtgtgccg agtttgatga tcgagatgag
     2881 gacgatgtga aggaattcag aggagctgag gaaaaccctg tgcgtaactc gcgaggaggg
     2941 accccaaccc cccgtcgttc ccgacgttct tccaccgtgc acacgtttcg gtgcagccgg
     3001 ccggttcggc tgggccggac ggagaggtcg cgagaggcgc tcgtctcgaa ggggcgctcc
     3061 ggcttctggt atcgtcggcg gcccgtgagg acgacgtgat catccggccc ttccgcggcg
     3121 cgaccccggt cctgggcgag agggtcttcc tcgccgagac ggcggcggtc gtcggagacg
     3181 tcgtcctcgg ggacgacgtc tcgatctggt acggctcggt ggttcgcggc gactgctgca
     3241 cgatccggat cggagcgcgg tcgaacgtcc aggacaactc gacgctccac gtcacgaagg
     3301 acgtcggccc cctcctcctc gaggaggagg tgacgctcgg ccacaactgc gtcgtccacg
     3361 cctgcacgat ccggcgcggc gcgctcgtcg ggatgaacgc gaccgtcctg gacggggccg
     3421 agatcgggga gagggccctc gtcgccgcgg gggccgtggt cctcggcggc accgtcgtcc
     3481 cgccgcggac gctctgggcg gggagccccg cgcggctccg ccgcccgctc accgacgcgg
     3541 aggccgaagg gctcgacgcg tactggcgga actacctgga atacaaggag gagtacctcc
     3601 gagaggacgt gcggcgcggg cggagcctcc cgctccccgt gcccgtcctc tgacgaaggg
     3661 ccggccccga tgatcacgag cgtcaaggga atgcgggacc tgctcccgcc cgacacgcgc
     3721 gtctggaacc gcctcgagga gacggcccgg accgtcttct ccctctacgg ctacgacgag
     3781 atccggacgc ccgtgctgga gccgacggag ctcttcgtgc ggagcgtcgg cgaggcgtcg
     3841 gacatcgtcc acaaggagat gtacgccttc accgaccggg gcgggcgcga cgtctcgctc
     3901 cggcccgaga acaccgcggg cgtggcgcgc gcctacgtcg agcaccagat gccgcaggcg
     3961 ggcggcgtga ggaagctcta ctacgtcgga ccgcagtacc gctacgagcg gccccagaag
     4021 gggcgctacc gcgagttccg gcagatcgga gcggaggtgc tcggcgcggc cgaccccgcg
     4081 acggacgcgg agctgctcgt gatgctcttc gacctcttca cccgcctcgg gttcacgggc
     4141 ctctcggtct cgctgaacag cgtcgggagc gaggagtcgc gcccggccta cgtcgaggcg
     4201 ctccgcgcct acctcgccgc gcacgagggg acgatggggg aggacgaccg gcggcgcctg
     4261 cgcgagaacc cgctccgcgt cctcgacacg aaggaccccg cgctcgcggc ggcgctcgcc
     4321 ggggcgccgc gggtcctcga ccacctcgat cccgcctcga aggcgcacca cgaggagctc
     4381 ctcgcactcc tcgaggcgag cggaatcccg taccgggtcg agccccggct cgtccggggt
     4441 ctcgactact acacgcgcac ggtcttcgaa gtgacggcgc aggggctcgg cgcgcaggac
     4501 gccctcctcg gcgggggtcg ctacgaccgg ctcgtgtcgg acctcggcgg gccgaggacg
     4561 cccggcatcg gattcgcgat cggagaggac cggctcgtcg acgtcctgcc ggagccgttc
     4621 cgccgcgcgg cgatcgacgc gttcgcgccc gtcgccgtca tggcgatcga cccgcccgac
     4681 cgcgcggccg ccctcgcgct cgccggcgag ctccgccgcg cgggcgtggc cgtcgacctc
     4741 gacccggccg ggaaggggcc gggccacgcg ctgaagaacg ccgcgaagcg cggctgccga
     4801 acggccgtcc tcgtgggcga gaaggagcgg cagtcgggcg tcgtggtcgt caaggacctc
     4861 gtggagaggt cccagaggga ggtgccgatc ggcgacctcc cgcgcacgct gaagggagag
     4921 aggccgtgag cgcaatcgtc gcccgcgtcc ccgccggctc gctccgcagg gaggacgccg
     4981 gacggacggt ccaggtcaag ggctgggtcc accgccgccg ggatttcggc gagctcgtct
     5041 tcgtcgacct gagggaccgg tccgggatcc tgcaggtcgt cttcgacgcc gagctcctcg
     5101 acgagcccga gctcctctcg cgcgccaagg agctgcgcgg ggagtacgtc ctcgccgtga
     5161 ccggcgcgat cgtcccgcgg gaaggctccg cccggaatcc cgacatgccg acgggcgacg
     5221 tcgagctccg ggccaccgcg ctcgagctcc tcaaccgcgc ggagacgccg cctttcgtga
     5281 tcgaggacga cgtccgcgcc tcggaagacc tccggctgaa gtaccgctac ctcgacctcc
     5341 gccggccgga gatgacgtcg aacctcgtga agcggagcga cgtgacgttc cggatccggc
     5401 aggtcctcca cgaggagggg ttcctcgagg tggagacgcc gatcctgacg aagtcgacgc
     5461 ccgagggggc gcgcgacttc ctcgtcccgt cgcgcgtcca ccccggtgag ttctacgcgc
     5521 tcccgcagtc cccgcagctc ttcaagcagc tcctcatggt cgcggggatg gatcgctact
     5581 accagatcgc gcgctgcttc cgcgacgagg acctccgggc cgaccggcag cccgagttca
     5641 cgcaggtcga cctcgagctg tcgttccccg acgaggagac ggtctacgcg ctgatcgaga
     5701 agctcttcgc ggcggtcttc ccggcgtacg ggatcccgtg cccggtcccc ttcccccgga
     5761 tcacccacgc cgaggcgatg gatcggttcg ggatcgaccg gcccgacacg cgcttcggcc
     5821 tcgagcttcg cgcgctcgac gtctcggcgt caccgtcgga gatcctccgc ggcgcggaga
     5881 ccgtcaaggg gatcgccgtc cccggaggcg cggccttctc gcgcaagcgg ctcgacgagc
     5941 tcgaggccgc ggcgaagcag ctcggcgccg cggggctcgt ctgggtgaag ctccaggcgg
     6001 agccccccgg gagctggggc tcgaacgcga agaagatgct cgacgacgcg acggtcgcgt
     6061 cggcccgggc cgcgctcgac gcgaaggacg gggacctcct cctcgtcgtc gcggggaaga
     6121 gaacgacggt gaacgacgtc ctcggagcgc ttcggctccg cgtcgcgcgc gaggagaagc
     6181 tcgtccccga ggaccgtttc gacttcctct gggtcaccga ctttccgctc ctcgagtggc
     6241 acccggagga cggccgctgg tacgcgacgc accatccgtt caccgcaccg cggcccgagg
     6301 acgtgcccct cctcgacacg gacccgggga aggtccacgc tcgggcctac gacgtcgtcc
     6361 tgaacgggat ggagctcggc ggcgggtcga tccggatcca ccagccggag gtccagtcga
     6421 aggtcttcgc gacgctcggc atcggacccg aggaggcgaa ggcgaagttc ggattcctcc
     6481 tcgaggcgtt ccgctacggc gcgccgccgc acggcggcct cgccctcggg ctcgaccgga
     6541 tgctgatggt cctgacgcgc tccgcctcga tccgcgacgt catcgcgttc ccgaagacgg
     6601 cctcggcgag ctgtctgatg acggagtcgc ccgggccggt cgcggacgcc cagctcgccg
     6661 agctcggcat ccgcctggcg ggagatgacg ggccgaagcc ggcctgagcc ccgcgccggc
     6721 cgcgccttcc gcttcgcggc ggccggcgtg ccgtgcgagg tggccgtcgg agagggagcg
     6781 ctcgcgcgcc tcgcggacgc tctcccgcgg atcgctccgg ggcgctggct cgtcgtgtcg
     6841 tccgcccccg tcctggcggc gcagggcgac cgcctccgga ccgcgctcgc ccgggcgcgg
     6901 agcgtcgacc cggagccgct cgtcgttccc gacggcgagc gggcgaagag ctgggcggag
     6961 ctcggccggc ttctctccgg gatggccgcg aggggcctgg cgcgcgacgg cgggatcgtg
     7021 gcgttcggcg gagggacggt cggcgacgtc tgcgggctcg ccgcatcgct cgcccttcgg
     7081 ggtgttcccg tcgtccaggt cccgacgacg ctcctggccg cggtcgacag cgctctcggc
     7141 gggaagacgg cggtgaacct cgccggcggc aagaacctcg ccggctcctt ccaccacccg
     7201 cggttcgttt gcgccgacac gcggctcctc gacacgctct ccgagcggga ccatcggtcg
     7261 ggcctggccg agatcgccaa gtccgcgctc ctctccgccc cgttctggcg gcgcctgccg
     7321 cgcctcgtcg agggcctcgt gtcgcgcgac cccctggcgc tcgcggaggc gatcgggcgc
     7381 tcgctccgcc tgaaggcctc gatcgtctcc cgcgatcccg acgaggccct cgggctgcgt
     7441 cacgtcctga acctcgggca cacgatcgct cacgcgctgg agggggcgtc cgcattccgg
     7501 ctgcgacacg gcgaggccgt cgcctggggg ctcctcgccg cccttcgact ctcctcggtc
     7561 cggggtctcc tgacgtcggc ccgcgcggag gccgcggcct cgacgctccg cggcctcctc
     7621 gcgccgccac ccctcgacgc gcgcgtccgc cggggcttcc cggagctcct ctcgcgcgac
     7681 aagaagaggg accgggcggg gctccgggac gtcctgctgg aggctcccgg aagaccgcgg
     7741 ctcgtgcgcg tcacggtggc ggagctcgcc gccagccttc cgagcggcga cggctagaat
     7801 ccgcgggtgg ccgcactgcc tcgccgaccc cggctgcgca tcgtcacggc gctgatcgcg
     7861 acactcgtcg tcgtctcgat cgtcccgctc gcgctctccg cggtccggct cgtctccatc
     7921 aaccgcgacg ccctcgagac cgcggaagct cagtacctga cccgaagcgc cgtgacgctc
     7981 tccgacgcga tcgacgccta cctgcgccgg atgctcgtcc aggtcaagaa gatcggggac
     8041 ggactcgagc tcgcgcgtgc agttgccccc ggcgaggacc cgttcgcgtt catcggccgc
     8101 acggggctca tcggccggta ccgcgacgcg gagccgagct tcctcgtcct ccgggccctc
     8161 gaccccgacc gccgcggcgg cgtcgtgcag cccgcgggcc tcgacccgtc cctcgagaag
     8221 gagctcgacc gggccttcga caccgcgctg cgcggctcgg tctacgtcgg cgacgccctc
     8281 cggattcccg ggctcccgga tcccggtctc gtcatggccg cgcccgttcc ggcaccggac
     8341 ggggagaccg tcggagtcgt ggaggcgttc gtctcgctcg cgccgatccg cgccctgctc
     8401 gagcaggagc gaaagcgcga cctcgtcgcc tacgtcgtcg atcgccgcgg gagcctcgtc
     8461 ctctcgagcg acccggcgat cttcggcggg aaggagatgc tcctggacgt ggagctcgtc
     8521 gccgagttcg tccagcatcc cgttcgcctg acgcggacat acccgcaggg agagggagag
     8581 acggtgcggc gggtcctcgg gacggtcgct ccggtcggtc cggcggagga cctcccggac
     8641 tggggcgtcc tcgtcgagaa ggacgtcgcg agggcgttcg cggcggcgac gcagatgacg
     8701 cggacgtcgt ggctcgtcgc ggccctcgcc gtcgcgctcg ccgtcgtcgc ggcgatcctc
     8761 gccgcgaacg ccctctccag gcccgtccgg gccctcgcgg aatccgtcca ccggatgtcg
     8821 gagggagacc tttcccagcg cgtggatgtg cccgggacgt acgagctcgc ggagctcgcc
     8881 gaggggttca accacatggg aggggagctc gagaagtcga tcgagaagct gaagctcgcc
     8941 gcgcgcgaga accaggagct cttcctcaac tcgatccggg ccctcgcggc cgccgtcgac
     9001 gcgaaggacc cgtacacccg cggccactcc gagagggtcg cgcgctactc cgtcgcgatc
     9061 gcccggcacc tgggccagcc gctcgaggag gtccggaagg tccggatcgc ggcgctcctg
     9121 cacgacgtcg ggaagatcgg catcgacgac cgcatcctgc gcaagccgac ggcgctcacg
     9181 gacgacgagt tcgagatcat gaagctgcac ccggtcaagg gggcgctcat aatgggccag
     9241 atcccccagc tgaagtggat catccccggg atgaagtacc accatgagaa gtgggacggg
     9301 accgggtacc cggacgggct gaggggagag gagatcccga tgctcgcgag gatcatctcg
     9361 gtggccgaca cgttcgacgc gatgacgacg acccgcccct accagaaggc gatgaggagc
     9421 gattacgtgg tctcccggat caagacgttc gccggcacgc ggtacgacac ggccgtcatc
     9481 gaggcgctcg agaaggcgct ccagaagaac gacctcgagg tgatcggcga ggccgcccgg
     9541 atggcggagg cgtcgtgagg ggcgtctgcg ccgtcacggc ggtcgtcgcg ctctgggtcc
     9601 tgagcgggtg ccagagcacg ctcgacgccg cgaagaagct cccggagccg agccctgccg
     9661 tcgccgacct cgtcgactac cggcaggggc tcgccctcct ccgggagggg cgcgtcgacg
     9721 aggcgatcca gcagctcggc tcggcccggg ccgcctaccc gcggagcgcg gaggtggcga
     9781 acgcgctcgg cctggcgtat ctctaccgga aggaccaccg gaacgcgacg aagctcttcg
     9841 ccgaagcgct cgagctcgac gagaacctcg tcgaggcgaa gaacaaccgg ggcgtcgcgg
     9901 cgatggaggc gggccggttc gaggacgcgg agaaggactt cggcgacgtg ctctccgggt
     9961 cgcgcacgtc cgagcacgtc aaggcgcggt acaacctcgg gctgctgcgc gcccgccagc
    10021 tccgctgggc cgacgcggac cgcgagctga cgaccgtcct cgccgacgac ccgggctacg
    10081 tcgacgcggc gcgcgagcgg ggtctcgcgc ggatgaagct cgaggacttc cggggggccc
    10141 tggaggactt cctcctctac ctccgcgaga acgggaacga cccggtcgcc aactacaacg
    10201 ccgccctctg cctcctgacg accgatcggc gggacctcgc cgtccgctac atggagagga
    10261 cggtccagtc cgcgcccgag agcgacgagg cgcgaaaggc gcgccgcttc ctcggcggcg
    10321 aggcgggagc ggcaggcagg gaacgatgag cttcgaagac gtcctccgtc cggtcctcga
    10381 cgcgcccggc gcgcaggccg tcgcgttcct cgacccgcag ggggaggcga tcgcgtccct
    10441 cggcgaccat gtcctcctcg agacgctggg cgcctaccag tccgtctgga cgacggagct
    10501 cgcgcgggcc gccatgggcg ggggcctcgg cgaggtcacc gaggtcgact tcgagttcga
    10561 cgcgggacgg gtgctcgcga ccgcggtccg ggagggctat ttcctcctcg ccctcttcgg
    10621 gcgggacggg gtgccgtccg tggcgcggcc gtccatcgcg gccgcccgca cgcggctcgc
    10681 ggccgagatc ggctgaaagg ggcagggacc gtggccgagg gagctcgcac gtcgctgatg
    10741 gcccgcctga agcgcgggct cttcatgacc cacacggaac tgatcgcgcg cgtcggcgac
    10801 gcgatcaagg cccgattctc gccggatccg aaggcgctcg aagccctcga ggaggcgctc
    10861 ctcgcggccg acgtcggacc ggcgaccgct tccgagctcg tcgaggcggt ccgggtcgag
    10921 gcggggaagc gcgacgcggg cgaggccgac gtcgtccgcc gcgtcctgaa ggccgagatc
    10981 gagcgacggc tctccgtcga gggaccggcg tccgccctgg tcccgccggg aatgccgcgc
    11041 gtcgtcctga tggtcggcgt caacgggacg gggaagacga cgacggcggc gaagctcgcc
    11101 gcgcgtgcgg ccgcggccgg ggggcgcccg gtcctcgccg ccgccgacac cttccgggcg
    11161 gccgcgatcg accagctcga ggtctgggcc ggacggatcg gcattccgct cgtgaagcac
    11221 cgtccgggcg ccgacccggc cgccgtcgtc ttcgacgcgt gcgccgcggc gaaggcgcgc
    11281 ggagccgatc tcctcctcgt cgacaccgcg ggacgcctcc acacgaagca caacctcatg
    11341 gaggagctct ccaagatccg gcggatcgcc gggcgggaga ttccgggggc gccccacgag
    11401 gtcctgctcg tcctcgacgc ggtgacgggg atgaacggcc tcgcccaggc ccgcgagttc
    11461 ctgaaggccg cgggcgtcac cggcctcgtc cttacgaaga tggacgggac cgcgaagggg
    11521 ggcgtcatcc tcgccatcgt ccgcgagctg aacctccccg tgcggtacgt cggcgtcgga
    11581 gagacggccg acgacctcct cgacttcgac ccggccggct tcgccgccgc gctgatcgat
    11641 gactgacctg gcctccgcct tcgagagggt cttcgagctc gccgagcggg ggcggtactc
    11701 cacctcgccg aacccgcgcg tcggcgcggt cgtcgtcgac gcggccggag ccgtggtcgg
    11761 cgaggggtgg cacgagaggg ccggaagccc gcacgcggag gcggtcgcac tcgcggaggc
    11821 ggggagccgg gcccgcggcg cgacggtcgt cgtgaacctc gagccgtgtg cccatcacgg
    11881 ccggacgccg ccctgcgccg atgccctcgt cgcggccggc gtcgcccggg tcgtcttctc
    11941 cgtcctcgat cccgacccgc ggacggcggg gaaaggacgc gagcgcctcc gcgaaggcgg
    12001 cgtcgcgacc gagacgggcg ccttcgccga gcgcgccgaa cgactgaacg agccgttcct
    12061 gacggccgcg aggagcggcc ggccgttcgt ccacctgaag tggggggcgt cgctcgacgg
    12121 gaagatcgcg acggcctcgg gcaggagccg ctgggtgacg tgcgaggagg cgcgcgccga
    12181 cgggatgcgg ctccgggagg agtgcgacgc catcctcgtc ggggcgggga cggtgcggac
    12241 cgacgacccc gcgctgaccc gccgggcggg cctgaaccgc tccgtcgtgc ctcaccgccg
    12301 cgtcgtcgtc gacggccggc ttcgggtccc cgccgaccgg aaggtcttct cgcccgccgc
    12361 gcccggcgag gcctggctcg ccacggccgt gcccgaggcc gacccgcgcc tcgccccgtt
    12421 ccgggacgcc ggggtccggg tcctctccct gccttcggcc gcgccgggtc gggtcgacct
    12481 cgccgcgctg ctcgtccggc tcgccgggag cgacgtccgg tcgctcctcc tcgagggggg
    12541 cggcgagacg gccttctcct tcctcgagtc gggcctcgtc gaccgcgtca cgggctacta
    12601 cgcgccgctc ctcctcggag ggcgggaggc accgtcggcc ctcggtggcg ccgggttccc
    12661 gaccctggag ggcgcctgcc gtctctcgca cctcgaggtc gcgccgctgg gggacggctt
    12721 ccgggtgagc gggcgtgtcg cccggccggc ggcgtaggat cggcccgtgt tcacgggact
    12781 cgtcgaggcc gtcgtcgagg tgcgggccgt cgggaggcgg gcggggggcg ccctgctgga
    12841 tgtcgagcgg ccggcaacat tcggagacgt cgcccgcggg gagagcatct ccgtctcggg
    12901 cgtctgcctc accgccgtcc ccggcgtcga agccgggaga ctccggttcg acgtctccgc
    12961 cgagacgctc gagcggtcga ccctcggcgc gcttcgtccc ggcgacgccg tgaacctcga
    13021 gcgggccctc gccgtcggcg gccgcctggg cgggcacgtc gtgcaggggc acgtcgacgc
    13081 gacggctccc gtcacgaggc tcgagaggag cggcgagttc tggaccctcg gggtccggct
    13141 cgatccctcc ttcgcgcgtt acgtcgtcga gaaagggtcc gtcgcgctgg acggcgtctc
    13201 cctgacggtc gcctccctcg cggaggagga gctgacggtc gccgtcatcc cggagacgtt
    13261 ccgcgcgacg acgctcggcc ggcgccgcgc gggagccctg ctgaacgtgg aggtcgacgt
    13321 tctcgcgaag tacgtcgagc gcctcctcgg ccgggccgcc gctccgtcat ccacgatgga
    13381 cgaccggctc cgggcgctcc tcggctcgtg acggcccgcc ccggcgccta cgtccacgtc
    13441 ccctactgcg cgcaccgctg cgcctactgc tcgttcgtgg cggtgacggg ccgggagacc
    13501 gagcgggact atttcgacgc cgtcgtggcc gagatcgaag gtcgagccgg cgaggtcgcc
    13561 ggcccgctcg agacggtcta cttcggcggc ggcacgccgt cgttcgccga tccggagagc
    13621 ctgctccggg tccttcacac gctgaggcgg acctggggac tcgtcccccg agccgaggtg
    13681 accgccgagg cgaacccgga cgacctcgtc ccggagcgcc tcgacgccct cgccggcctc
    13741 ggggtcaatc ggctctcggt cggcgtccag tcgctccgcg acgccgagct cgtcccgctc
    13801 gagcggcggc acgacgccgc ttccgcgcgg gacgccgtcc gccgcgcgct cggccggggc
    13861 ctccgggtct ccgccgacct gatgatcggc atcccgggcc agacgcgcga aggcctcctc
    13921 gaaggccttt cggagctcct tgccgccggc gtcggccacg tctcggtcta cctcctcgag
    13981 atcgagaagg cgccgcgcct cgtggctctc cgggagtcgg ccccggcgct cttcgcgggg
    14041 gacgacgaaa tggcggagcg atgggagcgc gtcgacgacg cctgccacgc cgccgggctg
    14101 ccgcgttacg agacctcgaa ctgggcgaga cccggcgagg agagccgcca caacctgaag
    14161 tactggcggc gggagccggt cgtcgcggtc ggcgtgtcgg cggcttcctt cgacggcctc
    14221 gtccggcgga cgaacggcgg gtcgatcccg gcctacatgg cggcgatccg ggagaagggg
    14281 acggccttcg tctccgagga gcggctcccg gccgacgcgg cgctccggga ggaggtgcta
    14341 ctcggtctcc gcacggtcga cggggtcgag gcccggacgt tcgaggccgc cctgggcacg
    14401 ctgcccgcgg ccgagaggga gcggctcgcg gacgccgtcg cggcgggcct cctcgagacg
    14461 gcggcgggcc gggtccgtct gacgcgaaga ggcgtcctcc tgtcgaacga ggtcttcgcc
    14521 ctcctcctct gagcacctcc ggcggccgtg tcgggcgcgg ggccggtccg gtccgcgggc
    14581 cgcttggcct cccgggccga gcgtgagacc atccgccggc tcgggaccaa gaaggacccg
    14641 gattcgtacg ttccggagcg gaggcgcatg aacgcagaga ctctggtcta tgtcatcccc
    14701 gtgttcggtc tcgcggggct tctctacacc tggctgaagt cgtcctgggt cgcccgtcag
    14761 gacgtgggca cggagcggat ggcgaagatc gccggggcga tccagcaggg cgccatggcg
    14821 ttcctccggg ccgagtaccg tgttctcggg gccttcgtcg tcgtcgtggc cctgctcctc
    14881 ggggtctccg gcgcgcttca ggacgggtcg agcccgctcg tcgcggccgc cttcgtcgcc
    14941 ggggcgctct gctcggcgct ggcggggctg atcgggatga aggtcgccac gaaggccaac
    15001 gtgaggacga cccaggcggc gcgccggggg ctcgggccgg cgctcgagat cgccttcgcg
    15061 ggcggctcgg tcatggggct cggggtcgtc ggtctcggcg tcctcgggct cggcggctgc
    15121 ttcctcgtct tctcgaagct cttcgcgggc gacgtccaca agacgatcgc cgtcatcacc
    15181 ggcttctcgt tcggagcgag ctcgatcgcc ctcttcgcac gggtgggcgg cggcatctac
    15241 acgaaggcgg ccgacgtcgg cgcggacctc gtcggaaagg tcgaggcggg aatccccgag
    15301 gaccacccgc tcaaccccgc gacgatcgcc gacaacgtcg gcgacaacgt gggcgacgtc
    15361 gccgggatgg gcgccgacct cttcgagtcg tacgtcgggt cgatcgtcgg ggcgatggtc
    15421 ctcggagccg cgttcatgac cggccgcctc gccacgggcg agccgttcac gacggacggc
    15481 ttcggaggcc tctcggccgt cctccttccc ctcgtcctcg cgggcgtcgg catcctcacg
    15541 tcgatcgccg gaacgttctt cgtccgggtg agggaagggg gctcgcccca gagggccctg
    15601 aacacgggcg agttcggctc gtcgatcgtg atggtcgtcc tctcgttctt catcatccgc
    15661 gggatgctcc ccgggagctt cgtcttcgac gacgcgctct acggcgcgga gcggacgatc
    15721 acctcgctcg gcgtcttctt ctcgacggtc atcggcctcg ccgccggcct cgccatcggc
    15781 gtcctcacgg agcactacac cggaaccggg accggcccgg tcctttccat cgcgaggcag
    15841 tcggtcacag gcgcggcgac gaacatcatc gcggggctcg gggtcgggat gcgctcgacg
    15901 gcctggccga tcctgatcct cgccgcttcg atcatcggcg ccttccattt cggaggcctc
    15961 tacggaatcg cgatcgcggc cgtcgggatg ctctcgaaca cgggcatcca gctcgccgtc
    16021 gacgcctacg ggccgatctc cgacaacgcc ggggggatcg ccgagatggc ggagctcccg
    16081 aaggacgtcc ggagccggac cgacaagctc gacgccgtcg gaaacacgac cgcggcgatc
    16141 gggaagggct tcgccatcgg ctcggccgcg ctgacggccc tcgccctctt cggcgccttc
    16201 atgaccgccg cgcacctgaa gacgatcgac gtctcgaagg ccgaggtcat ggcgggtctc
    16261 ctcgtcggcg cgatgctccc gttcctcttc tcctcgatgg cgatggccgc cgtgggccgg
    16321 gccgcgatgg cgatgatcca ggaggtccgg cgccagttca ccgagattcc ggcgctgaaa
    16381 tccgcgatct tcgcgatgga cgcgcactcc gggaaggact tcaaggactg gccggcggag
    16441 gaccagaaga tcttcgagga cgcgctcggg aaggccgagt acggcaagtg cgtcgagatc
    16501 tccacgacgg ccgcgatcaa ggagatggtc gcgccggggc tcctcgcggt catcgtgccg
    16561 gtcgtcgtcg gcttcggacc gaggctcctc ggcctcggct ccggcgcgga gatgctcggc
    16621 gggctcctgg cgggggtgac ggccacgggc gtcctcctcg cgatcttcca gtcgaactcg
    16681 ggaggcgcct gggacaacgc gaagaagatg ttcgaggagg gcgtcgagat cgacgggcac
    16741 cggtactaca agggatcgga cccccacaag gccgccgtcg tcggagacac cgtcggcgat
    16801 cccttcaagg acacctccgg tccgtccctg aacatcctgc tgaagctcat gtcggtcatc
    16861 gccctcgtca tcgcgaccct gctctgagct ccgggcgccg cggcggcgcc ctttcggtca
    16921 ccgagcggag cgaggccccg gcgagagccg gggcctcgcg gcgtcagggg ccgccgccac
    16981 ccaccctcga gcgggacaac gggaacgcga ggctcccgag gcgactcggg ccgaggcctg
    17041 ccggacgtcg tccggcaggg gaggggggcc gggagccggc gggcggggga accggattgg
    17101 ggcggccgga gcgggcggcc gggcctcgac gaggaaggcc ggaaaagcag cgtccccggc
    17161 cgtgaggccg gggacgcgat cgggagaccg tgtttcggcg ggcgagtccc gccggagcgg
    17221 aatcagctgc ccgcgggcgc gacctgctcg agggggttga cccagtccat gacgatgccg
    17281 atgttctcgg cgccgttgtt ccggacgacg tccatgagcc tcatggcgtc gtcgtacttg
    17341 accgtgtcct cgcaggagaa gaagacgggc ttcttgccgc ggttccggag gacctcctgg
    17401 aggcggatgg ggaggttcga ccgctcgacc cgctccttgt tgaggtagat ctcgttgttc
    17461 gcggtgagcg agacgatgat ctgctccggg ttcgcctggg gaagcacgtt cgagggggtg
    17521 ttgggcggga cgttgacgtc gtagcccatc tgcagcatgg gctgcaggat catgaagatg
    17581 atgaggagga ccagcatgac gtccacgagg ggcgtcacgt tgatctcgga cttgggcccg
    17641 cccttgctgc caccgatgtc catacccatg gcgtgccctc ctacttctcg gccttgcgct
    17701 cggtgatgag gccgatcttc tcgaagccgc cgtccttgat catccgcatg accttgcgga
    17761 cgtcgccgta ctggagcgac ttgtcggcct tgatcaggac gtccttgctc gcggcgcgct
    17821 caccgaactc gcgcatcttg gcagcgaact cctggtcggg ataccacttc gcgtcgatga
    17881 agatctgctt gtcggactgg acgctgatga ggagctcctt gtccgattcc ggtttcttgt
    17941 cgggctggtc cgtctgcggc agcaggacgg gcttgccctt ctggagcatg ggcgtgacaa
    18001 ccatgaagat gatgaggaga accagcatga cgtcgacgag aggcgtgacg ttgatctcgc
    18061 tcttcgtctc gtgccggcct ccgaagtctt ccattcccat cgtcgtgtcc tccgttcagt
    18121 tccgagaaga gcttgccggg gagccccggc gaggctctcc tcaggccgcc cgggagccca
    18181 tgcgcttgat gaagtagtcg aggagctccg acgaggagtt cgacatctcg acgttgaagc
    18241 gctcgatctt gttgaggaag tagttgtaga gccagaccgc cgggatggcg acgaagaggc
    18301 cgagagccgt gccgatgagg gcttccgcga taccggcgga gaccgcgccg aggccgccgg
    18361 tgccggaggt cgccatgccc tggaaggagc ggatgatgcc gaggaccgtc gcgaggaggc
    18421 cgatgaacgg cgccgtcgtg gcgatcgtgg cgaggccgcc gaggccgcgg cgcatgtcgg
    18481 cgcccgtcat catggcagcg cgggtgatcg cccgctcggc ggcggcgacg acgtcgtgac
    18541 cggcgacgtt tccgccgtgg gcctcatagg cgaactcgag aagaccgggc gacaccacct
    18601 tggcgacgtg gctggccttg tacttcttgg cgaggtcgat cgcctccttc agcttctcct
    18661 gcttgaggag cggcccgatc tcgagggcga acttcacgga ctgcttcttc gcgttccgga
    18721 aggcccacca gcgctcgagg gagaccccga gggagtagac cgacaggaac acgagaagaa
    18781 ccatgatcgc cttgaccgtg atggacatct gtgcccagat gtgtccgaga tccatttcca
    18841 tggtggagat cctccttgtt ggatttcagc gtcgcttgcg atcgcgtttg tgatcgggtt
    18901 actggagctt gaaggtcacc gtgaccgtca ggtagaccgg gaccgggcgg ccgttgaagg
    18961 tcgccggctt gtacttccac tgctggacgg cgcgcatcgc ggcgttgtcg agcagcgggt
    19021 tgataccgcg caggacgcgg accgactcga cgctcccggt cttcgtgatg atcgcctcga
    19081 ggatgacgat gccctggagt cgggccttgc gggccgcctc ggggtagacg ggctggacgc
    19141 gggagatctc gacgggctcc ttgacgtcgc cgccgacgcg caggggctcc tcgacctcgg
    19201 ccgcgccgcc gaggacgcca cccttcactc cgccgaggac gccgcccatg acgccgcctt
    19261 cgacgccgcc ctcgacgcct ccttcgacgc ctccttcgat gccggagtca tcggcctcgg
    19321 gggccgcggg agcgaccggg aggacgtcgg ggatgatcgt gggggccgcg ttggccgacg
    19381 gcttgaccgc ctcgaccttg ggggccgcct tgggggccgc ggccttgggg ggcggcggcg
    19441 gcggcggcgg cgggggagcg gcctctgtgt agaaggtgac cgggatcggc ggctcgggga
    19501 tgtcctcgat gtaccacatc gacgcggcga ggacggtccc gccgacgacg aagtggagga
    19561 agacggacat cggaaccgag agccagcgac tgccggtcgg cttctgcttc ttcgactcga
    19621 tgagggaagt ttcgaacatg gtcgtggtcc tctcgcccgg gcccgtcaga tcgcctcgag
    19681 cgggttcttc ctggccgctt cctcgcgctc cttctgcttg acgcggtcgc gcgcggcgag
    19741 ggccttcgtc tgccactcct tggccttctc gaggagctc
//
LOCUS       DAOONL010000065        19778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2745_length_19778_cov_16.102064, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000065 DAOONL010000000
VERSION     DAOONL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2745_length_19778_cov_16.102064"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLP50_10670"
     CDS             complement(<1..608)
                     /locus_tag="PLP50_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPA52052.1"
                     /translation="MRRLLLAVSLLLLAPAAPGAGTEVVVVDVDTDINPVTAEFVKRV
                     VEEAAAEGAPAVVLRINTPGGRLDSTREITKSILSSEVPVVGFVHPPGSQAASAGFII
                     LMACDVAAMAPGTNAGAASPVGGGGEELPKTIGKKVSEDAAALVRSLVVPRGRPVDDA
                     VKTITDALSYSESEAKEKGLVEIVARDVPDLLKQLDGRTIKRV"
     gene            629..1543
                     /locus_tag="PLP50_10675"
     CDS             629..1543
                     /locus_tag="PLP50_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPA52053.1"
                     /translation="MLQRAVRFALADLLTCGAAALLLAVGLAGGVPASAPAAFAKVAV
                     ALALPLVFAAVRGRRPAPGSLLASVADFGPILPLLLVFDNLGPFVLGAGSVDLDPLLV
                     AADRLLFGGDDPTRLLEPYAGPLLLEVLTVCYALYYFHPIVLGALLWKDDLREKTPGA
                     RFPAYVFGMLLVFYVSYAGYFAVPAIGPRFTVPHDGPLPRAAVAKAIDDTLDRLETSR
                     RNCFPSGHTMVTIAVLVEAARRSRKTFLAFLPFATGLVLATVFCRYHYVVDVLAGLVL
                     AFVVGGLARWLARRVPQEADGTVLATSR"
     gene            complement(1504..2652)
                     /locus_tag="PLP50_10680"
     CDS             complement(1504..2652)
                     /locus_tag="PLP50_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52054.1"
                     /translation="MAEGLSVLEVSSRRDLAEFVELPYRLFASEPDWVPPLRSDVRWM
                     LDPARNPFWNHARRSLLLARRGGRVVGRVAAIADDAHNRIHADRTAFFGFFECENDPE
                     AAKALFDAAEAAARRLLPGCDRLRGPVNPSMNDEVGFLLAGESEPGPPVLMMTWNPAY
                     YLELAAAAGFSKEKDLVALIAPIDDRSFARLGRITDAVRRRNPELTFRTIRMDRFPEE
                     LAKVKEVYNAAWERNWGFVPMTSEEIDAMAKKLKDLLYPPIVWFVEAGEKPVAFMLGM
                     PDFNQVLIRMGGRLLPFGWLRFLLGRKKVDRVRLMAFGVLPEYRRKGLDAVCYFEGYR
                     AAMAAGFRTVEFSWILEDNLDLLKPIEVFEGRLYRRYRLVARTVPSAS"
     gene            complement(2693..3931)
                     /locus_tag="PLP50_10685"
     CDS             complement(2693..3931)
                     /locus_tag="PLP50_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPA52055.1"
                     /translation="MRSSALKPAVAAPVRERVSILDKTRRYTVPDEIKAAGVWTYFRM
                     LESGQDPVVSMDGHEMVMLGSNNYLGLTSHPKVKEAAIAAVRKYGAGCAGSRFLNGTL
                     DIHVQLEERLAEFMRKPACITFSTGFLANLGAIGTLPGKGDTIYLDRQDHACIYDGAR
                     LAIGAEVRKFKHNDPDDLVRLLNGHPDKGGRLLVVDGVFSMEGDIAPVPAYAEICAEY
                     GMAFMIDDAHGIGVLGKTGRGTAEHFGLEDRADLIMGTFSKSLAAIGGFIVADADVVK
                     YIQHKARALIFTAAPPPASIGAVLAALDIIEAEPERRQHLWDNTRFMMTSLRAIGFDC
                     GESETPVIPIVVGEDYLAFKMAKRLEEEGVFVNPVVSPACPPGRALIRTSYMATHRRE
                     HLTRALEAFQKVGRELGLVG"
     gene            4158..5375
                     /locus_tag="PLP50_10690"
     CDS             4158..5375
                     /locus_tag="PLP50_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPA52056.1"
                     /translation="MSVSQLAKSIAESPTLRLNEQARLLREKGEAVVHLGAGEPKNKA
                     PIAAILAAASKLNTGDVKYTPADGTPGLKKQIIRYTEENYDRVVAPENVIVSAGAKQS
                     VFNILYSVLNPQDEVVILAPYWVSYPEMVKMCYGVPVIVKAEDGGFVPRPEDIEKSVG
                     SATRVIICNSPNNPSGVLYPESLVSWLVDFCERKGIWLIMDDIYHRLVFDGKKSPSCF
                     KYTKKDTETSKVIAVNGVSKLYGMTGFRIGWAIAPRPLVQIMTNVQGQITTTVSVLLQ
                     AAAEGAMSGLQSAVDSLRLQLENNRNVMLQELASFNGVKTIKPDGTYYCLPDFRAYSQ
                     KSVELSDFLLKKALVVTVPGREFGMEGYLRLSYAGTIKEIQEGIARMKWALDPSAPNE
                     IYVGDKKLVRDWL"
     gene            5381..7012
                     /gene="pckA"
                     /locus_tag="PLP50_10695"
     CDS             5381..7012
                     /gene="pckA"
                     /locus_tag="PLP50_10695"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPA52057.1"
                     /translation="MLEIKTPAEGEASARKADYGLANHGLSNLRMVYWNLPTEALYEE
                     ISFRREARIVAGGPVVAHTGKHTGRAANDKAVVKEASTEGHVWWGPYNRPYAPEKFND
                     LFNRLQGYLQGHDLFVQDVYAGADPAYRVPVRIITEKAWHSLFVRNMFVLPQSLEEYR
                     RHVPEFTVIAAPGFQGFPPIDGTNAQTFITLSFDQRVCLIGNTEYAGEIKKSVFTVLN
                     YLLPLDGVLSMHASANVGPKGDSALFFGLSGTGKTTLSADPNRSLIGDDEHGWSDEGV
                     FNIEGGCYAKVIALSPTAEPEIHSTTHRFGTILENVVFDPVTRKIDLDDETITENTRG
                     SYPLEFIANAVPEKKAGHPKNIIFLTCDAQGVMPPIARLTPDQALYQFISGYTSKIAG
                     TEAGLGKEPELTFSACFGGPFMVHHPYFYADLLKRKIERYGVSCWLLNTGWVGGPYNI
                     GKRISIRYTRALLNAALDGTLHGVEYVKDPVFGFEVPKSCPGIPDSVMDPSSSWPDKA
                     AYQTRYRSLAARFVENFKKFEEGCAPEVIAAGPKA"
     gene            7171..9018
                     /locus_tag="PLP50_10700"
     CDS             7171..9018
                     /locus_tag="PLP50_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPA52058.1"
                     /translation="MTLTRDSGGAAPTSGRDGWLFVLPLLSTPLLTAAIGTAVRAMAG
                     SGQGRALPPGEIAAQLVVGYALLALSRHPLPFLFAQAVVIAIPHAAQALRVAILAGPI
                     RLADLRAAPELVRVLDLRWTMSLAGPFCVLAALLFANRGFRRGPAVAAAGLSALLAGA
                     LFVRPAAVAALADGGRPYVPWSHVDNMRQQGPVGYLLGELARQRIERQGPPTIDAVRA
                     ALAEAPARPREGAGSGASRSRRPVVLILLESFWDPARLRSAGLSGDPFAPRFRELWSA
                     AGESAALSPEFGGATANAELEVLCGIPTRLVFPGVVFASGLASDLPCLPRLLARGGLR
                     AEAHHPNVPSFWSRARAYPRLGFTRFFSAADFDLDDLNGDLLSDESLYRQAWSRTRKG
                     PPGSVALTYVLTYTGHWPYDLNPARRAYEISARSAPEVVGRYASSVLHSSRELAAFVD
                     RILDDEPDALVVALGDHLPSLGATDEAYRKSGLVDDPDAGLSASTFRSLTTVPLLVVD
                     GKAGPLPLGTISQFELPALVLERLGLPVPAWMSALLPPPGWHVRTREEGLLVLPPDGT
                     EHYCRTPAEGPACDVGFRWLERARVLSRDLVEGRQHALALLGDVSAPPE"
     gene            9061..10755
                     /locus_tag="PLP50_10705"
     CDS             9061..10755
                     /locus_tag="PLP50_10705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPA52059.1"
                     /translation="MRLALALALLLSPLGARAERPADLLLTGGKVWTADAETPLAEAV
                     AVRDGKVVFVGSDAEAAAWRGPKTEVIPLGGRLVTPGFEDAHLHLMSGARNLERVDLS
                     GEATLAGLQERIRRFAAANPRSPWVVGRGWVYGTFPGGLPTKELLDAVVPDRPAYMDG
                     YDGHTGWANSRALALAGITKETKDPVNGTIVRDPRSGEPTGALKEAAGDLVEDLVPEP
                     DAERHYALLLNALGILNRGGITAIQDAGWTKDEAARFLPLLERARKEGKLTVRARLTV
                     DLDPAEPAPGIAEAVRLRGLYRDGPIVFGGVKGYVDGVIEAHTAAMLAPYSDDTTLGA
                     GTPRWDGAALESAVAAADRRGLQVWLHAIGDRGIRMALDAYEAAAKANGPRDRRHRVE
                     HVEAVSAADAPRFGALGVVAAMQPLHANPDQNNADVWSKNVGPERESRGFAWRLVEGS
                     GGRIAFGSDWPVVAPDVIRGVSCAVTRKTPEGTPAGGWQPHLALTPESALRHYTIDAA
                     FAGFAEKERGSISVGKRADLVVLSRDLLSGPPEGILQTRALLTLFGGKPVWRDPSF"
     gene            10953..11528
                     /locus_tag="PLP50_10710"
     CDS             10953..11528
                     /locus_tag="PLP50_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52060.1"
                     /translation="MPPPLPIPECYWVVPGLLLAGEYPFSLVEDDGRERLRAFLDAGI
                     RLFVDLTEVRESGRLGALEPYAETLLEEAAARGVSVRVVRHAVRDMSVPRDGTLERIV
                     EELEGAVARREPAYVHCWGGIGRTGTAVAAFLSRRLGLTGDEALDLLDALWQEVPKSA
                     VFPESPQTDEQRRAVRAFAGRRGPAGPGEGG"
     gene            11525..12310
                     /locus_tag="PLP50_10715"
     CDS             11525..12310
                     /locus_tag="PLP50_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPA52061.1"
                     /translation="MSSRIESPTNPRVAAASRAVREGEALLLEGARTLRDALDAGVVP
                     DVVFAAPDLAPIDAAVAEDARAAGASVVEVSQRVLARLSELGSTRALVALAPVPSARL
                     ESLSRAALVLLLDGLQDPANVGAILRAAEAFGAGGVILTPGTASPYAPKAFRASSGSA
                     LRVPVVRNVLSTDAVRWVRSRGALLIGADAHGGEEPTALAGVAPLVLAIGSEGHGLSA
                     PLVAALERRVTIPLSPRVESLNAAVATGILLYALSPKTSAEKR"
     gene            complement(12271..12828)
                     /gene="amrA"
                     /locus_tag="PLP50_10720"
     CDS             complement(12271..12828)
                     /gene="amrA"
                     /locus_tag="PLP50_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPA52062.1"
                     /translation="MLTDAERDDLIQRARHAAARALGLPEGKRELPPPAGRLAEPGAS
                     FVTWRRDGRLRGCIGSVEPWRPLAEDVETNAVAALVRDPRFAPAQARDLRWLSVEVSV
                     LSPLDEVSDPLRQLEIGVHGVVVRKGSRSGLLLPQVAPEWGWDVPTLLAQVCLKAGLP
                     EDSWKEGSPPAALYRFSAEVFGESA"
     gene            complement(12846..13499)
                     /locus_tag="PLP50_10725"
     CDS             complement(12846..13499)
                     /locus_tag="PLP50_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA52063.1"
                     /translation="MSTVRMQSLTLPGPAGDLEALLRVPVRPRGAAVVAHPHPLFGGT
                     MHTKVVHRAAKVFSDRLHLSALRFNFRGVGASAGSYDEGRGETDDLVAAVHWVRERQP
                     SGPLALAGFSFGSLCAARAAVSVAPHVLLLIGVPLDRWDEEEARALEGRNVVWVQGGE
                     DSFGAGALALETAERHGFRAASVPGADHFFTGHLEGFEAAAVRLLAEAGFGAPVVPA"
     gene            13527..15272
                     /locus_tag="PLP50_10730"
     CDS             13527..15272
                     /locus_tag="PLP50_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA52064.1"
                     /translation="MNEVTEGTGTALALPAPSPRLERAIVLALVVLSAALALWHLAGD
                     AATADEPVHVAAAVEIAREGTGRWNPEHPPLAKALAGLSLAGLPLRPADDPLRSAAGP
                     ARLLRFLFANETPAETILFRARLPFVALLAALLLAVRGEARRRWGPWAGITALAFAAL
                     EPNLNAHAGVVHTDLAVTLFVVLSLSPLARLARPEARRATALLGLLWGLAFLSKYSAP
                     LLALATLPFLAADATPRRQDFGRLLRRLAAAASIALAVTLAGFAWAGRSQTPEDRRAI
                     AVERLELKGRSPAAARLAIRAGDLLPPLGNVLTGALSVALQSEVGAGVNYLLGTVSRE
                     GSPWYFPLALAVKTPLGLLLGLGVALSARPGRRLAAVAGTALLLFLLLSSRTTYNIGV
                     RHALLAFPLFAITAGAAAAGRADPADRRSASLRRGAAVGALLVAALEAAVAHPHPFSF
                     FNALAGGAEGGRRFFADSSLDWGQDLLRLKAVAPALAPDGLATIVFGGDLPSRHAPLL
                     RPPRPGEEDRPGAILAIGEAPFALGPELLRAKGATGDADRLESIRTALRERGRRIGTV
                     GGSIGVWRIESAPPR"
     gene            complement(15282..16769)
                     /locus_tag="PLP50_10735"
     CDS             complement(15282..16769)
                     /locus_tag="PLP50_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA52065.1"
                     /translation="MVRRSARAVVLLSVALGACGAQPPPPALLVAMEQDVRTLDPHRH
                     DDSVTHSVLANVYEPLVTFDPQMRVVPALAEGWSNPNDLTWRFRLRPRVRFHDGLPLT
                     ARDAKFSLERARRFRAAPHLESLERIEAVNDETLEIGTSVPEPVLLNKLAGVGIVPAG
                     TPEEVTVPVGTAAYRFVSWVPGKELRLVANERYRDGAPPIREARFRVLPDATARAAAL
                     AAGELHLARELKRKDLDGAGKNVRFVSHPGLVVVLLGTRFDVPGPLRSRDVRRAIYLA
                     IDPQELVDLSGVEAVPVDQVVPPSVFGHLPDGGEPRPDPDRARALLRRAGLGEGFDVT
                     LDMAEAFAPSVGRVLAAQLARVGIRTTVASLPWHILSARLERGESPFFSVGWSCNGDA
                     SHLFDALLHTRQGRSWGVSNFGGYANPRLDEAVERAGAILSPARRLDALHEAMRVVLE
                     DLPLIPLFNRRRTYGVDARLEFRPRLNGQVILRELSWVDGPAGRP"
     gene            complement(16839..17603)
                     /locus_tag="PLP50_10740"
     CDS             complement(16839..17603)
                     /locus_tag="PLP50_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPA52066.1"
                     /translation="MSQEDQKLALFIDIDNVIIGLRESGHPKFDVKLLLSRLLDKGKI
                     VIKKAYADWSRYAEYKRAFHESAFELIDIPQHKMTGKNSADIRMVVDAMDLASNKEHI
                     TTFVIGSGDSDFSPLVSKLKENNKTVLGFGVKGSTSELLIENCDEFIYYEDLVREQVK
                     VPAVAGLPEKSREVFSLLIESMQALQRENKEILWGSMVKQTMQRKNPSFSESYYGFRA
                     FSDLLESAQKHRVITLRRDAKSGSYVITGYGPGTAR"
     gene            17829..18824
                     /locus_tag="PLP50_10745"
     CDS             17829..18824
                     /locus_tag="PLP50_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPA52067.1"
                     /translation="MPISDSSNEGVEALRAAVVAVDVTGRGALAVSGPDAAELLHGLV
                     TNEVKALPPGSGCHAALLTPKGRMRAELAVLRVSESEVLLDCDPSLAEPLLGILQGYV
                     PFSRSVLRDQTSETGVVHLEGPTAAGVLLAAALPEPAPDDYAHAAGSLDGVPVRVVRV
                     SRAGEPGFDVRTSPTAVPALLDLLSSRGAALVSPDVLEAGRIEAGLPRWGAELTETTL
                     PNEAGLERTAISYTKGCYLGQETVARIRTYGHVNRRLVALLLPIGSAAAPGDDVRAGE
                     ETVGNVTSAVDSSRRAARVALAYVKKGHDEPGTALAVATKEGAAAGRVAAVPLGR"
     gene            18814..19731
                     /locus_tag="PLP50_10750"
     CDS             18814..19731
                     /locus_tag="PLP50_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA52068.1"
                     /translation="MGAETRAREPGAAPYVALLFVQLFFGTLPVFGKIAMREMEPFAL
                     AAFRALFGALFLSLAARAFSGPEPRQTLREKGEVALLSLLGVVANQVLFITGLSLTTA
                     THATLLVATVPVFTILVGLLSRRERPSPRRLLAVPIAFGGVLFLLDPANLSFRHGTFV
                     GDLLVTLNSVAYAWFLVRARDVLVRRPALSFVSQAFRYGALPILLLALPGLSRLRPAE
                     LSGPALGSLVGVVLLGTVGSYALNAWALARTSASTTAFFIYVQPVFAGAFAFFALGEE
                     PSPRIFGAAALIFAGVALAIWPARKPPAS"
BASE COUNT         2648 a         7498 c         6743 g         2889 t
ORIGIN      
        1 acccgcttga tcgtccggcc gtcgagctgc ttcaggaggt cggggacgtc gcgcgccacg
       61 atctcgacga gccccttctc cttcgcctcg gactccgagt aggagagcgc gtcggtgatc
      121 gtcttcaccg cgtcgtccac gggcctcccg cgcgggacga cgagcgacct cacgagggcg
      181 gcggcgtcct cgctcacctt cttgccgatc gtcttcggga gctcctcgcc tccgccgccg
      241 accggagagg ccgcgccggc gttcgtcccg ggggccatcg ccgcgacgtc gcacgccatc
      301 aggatgatga agccggccga ggcggcctgg gagccgggcg ggtggacgaa gccgacgacc
      361 gggacctccg acgagaggat cgacttcgtg atctcccggg tcgagtcgag ccggccgccc
      421 ggggtgttga tccggaggac gacggcgggg gccccctcgg cggccgcctc ctccacgacc
      481 ctcttcacga actcggccgt gacggggttg atgtccgtgt cgacgtcgac gacgaccacc
      541 tccgtccccg ccccgggcgc ggccggagcg agcagaagga gagagaccgc gaggaggagc
      601 cgtcgcatcg gccgattata ggttcggcgt gctacaacgc gccgtgcgct tcgcgctcgc
      661 cgacctcctg acgtgcgggg ccgcagccct ccttctcgcc gtcgggctcg ccggcggcgt
      721 cccggcctcg gcgccggcag cgttcgcgaa ggtcgcggtg gcgctcgccc tcccgctcgt
      781 cttcgccgcg gtccgcggcc gccgccccgc tccggggtcc ctcctggcga gcgtcgccga
      841 cttcgggccg atcctcccgc tcctcctcgt cttcgacaac cttggcccgt tcgtcctggg
      901 cgccggctcg gtcgacctcg acccgctcct cgtcgcggcg gaccggctcc tcttcggcgg
      961 cgacgacccg acgcggctcc tcgagccgta cgcggggccg ctcctcctcg aggtcctcac
     1021 cgtctgctac gcgctctatt acttccaccc catcgtcctc ggcgcgctcc tctggaagga
     1081 cgacctccgc gagaagaccc ccggcgcgcg ctttcctgcc tacgtcttcg gcatgcttct
     1141 cgtcttctat gtcagctacg ccggctactt cgccgtcccg gcgatcggcc cgcgtttcac
     1201 ggtcccccac gacgggccgc tcccgcgcgc ggcggtcgcg aaggcgatcg acgacaccct
     1261 cgaccgcctc gagacgagcc ggaggaactg cttcccttcg ggacacacga tggtgacgat
     1321 cgcggttctc gtcgaggcgg cccggcgttc gcggaagacg ttcctcgcct tcctcccgtt
     1381 cgcgaccggg ctcgtcctcg ccacggtttt ctgccggtac cactacgtcg tcgacgtcct
     1441 cgccggtctc gtcctcgcgt tcgtcgtcgg cggcctcgcg cggtggctcg cccggcgggt
     1501 ccctcaggag gccgacggaa ccgtcctggc gacgagccgg tagcgccggt agagcctccc
     1561 ctcgaagacc tcgatcggct tcaggaggtc gaggttgtcc tccaggatcc aggagaactc
     1621 cacggtccgg aagccggccg ccatggcggc cctgtagccc tcgaagtagc agaccgcgtc
     1681 gagccctttc cgcctgtact cgggcaggac cccgaacgcc atgaggcgga cccggtcgac
     1741 cttcttccgc ccgaggagga acctcagcca gccgaacggc aggagcctcc ctcccatccg
     1801 gatcaggacc tggttgaagt cgggcatccc gagcatgaac gcgaccggct tctccccggc
     1861 ctcgacgaac cagacgatcg gcggatagag gaggtccttc agcttcttcg ccatggcgtc
     1921 gatctcttcc gacgtcatcg gcacgaagcc ccagttccgc tcccaggcgg cgttgtagac
     1981 ctccttgacc ttcgcgagct cctccggaaa gcggtccatc cggatcgtcc ggaacgtcag
     2041 ctcggggttc ctccgacgga ccgcgtcggt gatccggccg aggcgggcga aggagcggtc
     2101 gtcgatcggc gcgatcagcg cgacgaggtc cttctccttc gagaagccgg ccgcagccgc
     2161 cagctcgagg tagtacgccg ggttccaggt catcatcagg accggcggcc ccggctcgct
     2221 ctcgccggcg aggaggaagc cgacctcgtc gttcatcgac gggttcacag gcccgcgaag
     2281 gcggtcgcac cccgggagga gccggcgggc cgccgcttcc gccgcgtcga agagcgcctt
     2341 cgccgcctcc gggtcgttct cgcactcgaa gaagccgaag aacgccgtcc ggtcggcgtg
     2401 gatgcggttg tgcgcgtcgt cggcgatggc ggcgacccgc ccgacgaccc tcccgccacg
     2461 ccgggcgagg aggagggaac gccgcgcatg gttccagaac gggttcctcg ccgggtcgag
     2521 catccagcgg acgtccgagc ggagcggcgg cacccagtcg ggctcggacg cgaagagccg
     2581 atacgggagc tcgacgaact ccgcgaggtc gcgccgggag gagacctcga ggacggacag
     2641 cccttcggcc acgctccggt ccctcccgat ccggcccggc cccggtcggc gcttagccga
     2701 cgaggccgag ctcgcgcccg actttctgga acgcctcgag ggcccgcgtc aggtgctccc
     2761 gcctgtgcgt cgccatgtac gacgtccgga tgagcgctcg tcccggcggg caggccgggc
     2821 tgacgaccgg attcacgaag accccttctt cctcgagacg cttggccatc ttgaacgcga
     2881 ggtagtcctc gccgacgacg atcgggatga ccggcgtctc ggactcgccg cagtcgaagc
     2941 cgatcgcccg gaggctcgtc atcatgaagc gggtgttgtc ccagaggtgc tgccgccgct
     3001 ccggctccgc ctcgatgatg tcgagcgcgg cgaggacggc cccgatcgac gccggagggg
     3061 gcgccgccgt gaagatcagc gcccgcgcct tgtgctggat gtacttgacg acgtccgcgt
     3121 cggccacgat gaagccgccg atcgccgcca gcgacttcga gaacgtcccc atgatgaggt
     3181 ccgcgcggtc ctcgaggccg aagtgctccg cggtgccgcg cccggtcttg ccgaggacgc
     3241 cgattccgtg ggcgtcgtcg atcatgaacg ccattccgta ctcggcgcag atctcggcgt
     3301 aggccgggac cggagcgatg tcgccctcca tcgagaagac gccgtccacg accaggagcc
     3361 tgcctccctt gtcggggtgg ccgttcagga ggcggacgag gtcgtccggg tcgttgtgct
     3421 tgaacttccg gacctccgca ccgatcgcca gccgggcccc gtcgtagatg caggcgtgat
     3481 cctggcggtc gaggtagatc gtgtcgccct tgccagggag cgtcccgatc gcccccaggt
     3541 tcgcgaggaa cccggtggag aaggtgatgc aggccggctt gcgcatgaac tcggccagcc
     3601 gctcctcgag ctgcacgtgg atgtcgagcg tcccgttcag gaaccggctg cccgcacagc
     3661 cggcgccgta cttcctcacc gccgcgatcg cggcttcctt gaccttcggg tgagaggtca
     3721 ggccgaggta gttgttcgaa cccagcatga ccatttcgtg gccgtccatg ctgaccacgg
     3781 ggtcctgccc cgattcgagc atccggaaat aggtccagac gccggccgct ttgatctcgt
     3841 cgggcaccgt gtagcggcgc gtcttgtcca ggatcgagac ccgctcccga acaggggcgg
     3901 cgaccgcggg cttgagcgcg gaactcctca tactcctccg ccagcgtcgc cgcccaccgg
     3961 ggccttccgg gcgacccgca aactccgccg atcttagcac cggcccggga ccccggccgg
     4021 aacggctccc gaacaccccc gcccccctcg cgccggacgc gtaaacacct tccagaccgg
     4081 tacaatcccc cgttgccctg ggtgtcgcgg agcacggctc cagcggccca ccggcgaaaa
     4141 gggaggttcc caacgacatg agcgtaagtc agctcgcgaa gtccatcgcg gagtcgccga
     4201 cgctccgcct gaacgagcag gcccggctcc tgcgcgagaa gggcgaggcg gtcgtccatc
     4261 tcggcgcggg cgagccgaag aacaaggctc cgatcgccgc gatcctcgcg gctgcgtcga
     4321 agctgaacac cggcgacgtc aagtacacgc cggcggacgg cacgccgggg ttgaagaagc
     4381 agatcatccg ctacacggag gagaactacg accgcgtcgt cgcccccgag aacgtcatcg
     4441 tctcggccgg ggcgaagcag tcggtcttca acatcctcta ctcggtcctg aacccgcagg
     4501 acgaggtcgt catcctcgcc ccgtactggg tcagctaccc cgagatggtg aagatgtgct
     4561 acggcgtccc ggtcatcgtg aaggccgagg acggcggctt cgtgccgcgc ccggaggaca
     4621 tcgagaagtc cgtcggctcc gcgacgcgcg tcatcatctg caacagcccg aacaacccct
     4681 cgggagtcct ctaccccgag agcctcgtct cctggctcgt cgacttctgc gagcgcaagg
     4741 ggatctggct gatcatggac gacatctacc accggctcgt cttcgacggc aagaagtcgc
     4801 cgagctgctt caagtacacg aagaaggaca ccgagacgtc caaggtcatc gccgtcaacg
     4861 gcgtctcgaa gctctacggc atgaccgggt tccggatcgg ctgggcaatc gcgccgcggc
     4921 cgctcgtcca gatcatgacg aacgtccagg ggcagatcac gacgaccgtc tccgtcctcc
     4981 tgcaggcggc ggcggaaggg gcgatgtcgg gactccagag cgccgtcgac agcctccggc
     5041 tgcagctcga gaacaaccgg aacgtcatgc tccaggagct cgcctcgttc aacggggtga
     5101 agacgatcaa gccggacggc acgtactact gcctccccga cttccgggcc tactcgcaga
     5161 agtcggtcga gctgtcggac ttcctcctga agaaggcgct cgtcgtcacc gtccccggcc
     5221 gggagttcgg gatggagggc tacctccgcc tctcgtacgc cgggacgatc aaggagatcc
     5281 aggaggggat cgcccgcatg aagtgggcgc tcgacccgtc cgcccccaac gagatctacg
     5341 ttggagacaa gaagctcgtg agggactggc tgtgaagaac ctgctcgaga tcaagacccc
     5401 ggccgaggga gaagcctcgg cgcggaaggc cgactacggc ctggcgaacc acgggctctc
     5461 gaacctccgg atggtctact ggaacctccc gaccgaggcg ctctacgagg agatctcctt
     5521 ccgccgcgag gcgcggatcg tcgcgggcgg ccccgtcgtc gcccacaccg gcaagcacac
     5581 cggccgcgcc gcgaacgaca aggccgtcgt gaaggaagcc tccaccgaag ggcacgtctg
     5641 gtggggcccg tacaaccgcc cctacgcccc cgagaagttc aacgacctct tcaaccgcct
     5701 gcagggctac ctgcaggggc acgacctctt cgtccaggac gtctatgccg gggccgaccc
     5761 ggcctaccgc gtcccggtcc ggatcatcac cgagaaggcg tggcactcgc tcttcgtgag
     5821 gaacatgttc gtcctcccgc agagcctcga ggagtaccgc cggcacgtgc cggagttcac
     5881 cgtcatcgcg gcgccgggct tccagggctt cccgccgatc gacgggacga acgcccagac
     5941 gttcatcacc ctgagcttcg accagcgcgt ctgcctcatc gggaacaccg agtacgcggg
     6001 cgagatcaag aagtcggtct tcaccgtcct caactacctc ctcccgctcg acggcgtcct
     6061 ctccatgcac gcctccgcga acgtcggccc gaagggcgac tcggcccttt tcttcggcct
     6121 ctccggcacc ggcaagacga cgctctcggc cgacccgaac cggagcctca tcggcgacga
     6181 cgagcacggc tggagcgacg aaggcgtctt caacatcgag gggggctgct acgcgaaggt
     6241 catcgccctc tcgccgacgg ccgagcccga gatccactcc acgacccacc gtttcggcac
     6301 gatcctcgag aacgtcgtct tcgacccggt cacccggaag atcgacctcg acgacgagac
     6361 gatcaccgag aacacccgcg gctcctaccc gctcgagttc atcgcgaacg ccgtccccga
     6421 gaagaaggcc ggccacccga agaacatcat cttcctcacg tgcgacgcgc agggcgtcat
     6481 gcccccgatc gcgcgcctca cccccgacca ggcgctctac cagttcatct cgggctacac
     6541 gtcgaagatc gccggcaccg aggccggcct cggcaaggag cccgagctga cgttctcggc
     6601 ctgcttcggc gggccgttca tggtccacca cccctacttc tacgccgacc tcctgaagcg
     6661 aaagatcgag cgctacggcg tcagctgctg gctcctgaac accggctggg tcggcggccc
     6721 gtacaacatc ggtaagcgga tctccatccg ctacacccgc gcgctcctga acgccgccct
     6781 cgacggcacg ctccacggcg tcgagtacgt gaaggacccc gtcttcggct tcgaggtccc
     6841 gaagagctgc cccggcatcc ccgactccgt catggacccg tccagctcct ggcccgacaa
     6901 ggcggcctac cagacccgct atcgctcgct cgccgcccgg ttcgtcgaga acttcaagaa
     6961 gttcgaggag ggctgcgccc ccgaggtcat cgccgcgggg ccgaaagcct gacgatcggc
     7021 acgatctgac gcgacccggt ttcgagggga gcccgcgaga gcgggctccc ctttctcctc
     7081 aatcgtcgcg ccgtgatcgt cgccgcatcg gttaatgtcg cggccagcac gacgcggtgg
     7141 tgaaggaatc cccgccggga ggttcgcccg gtgactctga ctcgagacag cggtggcgct
     7201 gcgcccactt ccgggcgcga cgggtggctc ttcgtcctgc cgctcctgtc gactcccctc
     7261 ctgaccgccg cgatcgggac ggcggtccgt gcgatggccg ggtcggggca aggccgcgcg
     7321 ctcccgccgg gcgagatcgc cgcgcagctc gtcgtcggct acgccctcct cgccctctcg
     7381 cggcaccctc tccccttcct cttcgcgcag gccgtcgtca tcgccatccc gcacgcggcc
     7441 caggcgctcc gcgtcgcgat cctcgccggg ccgattcgcc tcgccgacct ccgggcggct
     7501 cccgagctgg tgcgggtcct ggacctccgc tggacgatgt cgctcgccgg gccgttctgc
     7561 gtcctcgccg cgcttctctt cgcgaaccgc gggttccggc gtggccccgc cgtggcggcg
     7621 gccggcctgt ccgcgctcct cgccggggcc ctcttcgtcc gcccggccgc cgtcgccgcc
     7681 ctcgcggacg gcggtcgacc gtacgtgccc tggagccacg tcgacaacat gcgccagcag
     7741 gggcccgtcg gctaccttct cggcgagctg gcccggcaga ggatcgagcg ccaggggccg
     7801 ccgacgatcg acgccgttcg ggccgccctc gccgaggccc ccgcgcgccc ccgcgaggga
     7861 gccgggagtg gggcgtcgcg ctcccgccgg cccgtcgtcc tgatcctcct cgagtcgttc
     7921 tgggacccgg cccggctccg ttccgcgggc ctgagcggag acccgttcgc gccacgcttc
     7981 cgcgagctct ggtcggctgc cggcgagagc gccgccctca gcccggagtt cgggggcgcg
     8041 acggcgaacg ccgagctgga ggtgctctgc ggcatcccga cgcgcctcgt cttcccgggc
     8101 gtcgtcttcg cgtcgggcct cgcgtccgat ctgccgtgcc tcccgaggct cctggcgcgc
     8161 ggaggccttc gggcggaggc ccatcacccg aacgtcccct cgttctggag ccgcgcccgc
     8221 gcctaccccc gtctcggctt tacgaggttc ttctcggcgg ccgacttcga cctggacgac
     8281 ctcaacggcg acctcctctc cgacgagtcc ctctaccgcc aggcgtggtc gcggacgcgg
     8341 aaaggcccgc ccggctccgt cgccctcacg tacgtcctca cgtacaccgg gcactggccc
     8401 tacgacctga acccggcgcg ccgcgcctat gagatctcgg cccgatccgc cccggaggtg
     8461 gtggggcggt acgcgagctc ggtcctccac tcctcccggg agctcgccgc gttcgtcgac
     8521 cggatcctcg acgacgagcc ggacgcgctc gtcgtcgccc tcggcgacca cctcccgtcc
     8581 ctcggagcga ccgacgaggc ctaccgcaag tccggcctcg tggacgaccc ggacgccggc
     8641 ctgtcggcgt cgacgttccg gagcctgacg accgtgccgc ttctcgtcgt cgacgggaag
     8701 gccggcccgc tcccgctggg gacgatcagc cagttcgagc tccccgccct cgtcctcgag
     8761 cggctcgggc ttccggtccc cgcgtggatg tccgcgctcc tgccgccgcc cggctggcac
     8821 gtgaggacgc gcgaggaagg gctgctcgtc ctgccgccgg acgggacgga gcactactgc
     8881 cggacgccgg cagaggggcc ggcctgcgac gtcgggttcc ggtggctcga gagggcacgc
     8941 gtcctctctc gcgacctcgt ggaagggcgg cagcacgccc tggccctctt gggggacgtc
     9001 agcgctccgc cggagtgacg gctacgccgg gcggcgcgca gaatcgcccc ggaggaaccg
     9061 atgagactcg cgttggccct cgcgctcctg ctctcgccgc tcggcgcccg cgcggagcgg
     9121 cccgcggacc tgctcctcac gggaggaaag gtctggacgg ccgacgccga gacgccgctc
     9181 gcggaggccg tcgcggtgag agacggcaaa gtcgtcttcg tcgggagcga cgcggaggcc
     9241 gcggcgtgga gagggccgaa gaccgaggtg attccgctcg gcgggcgcct cgtcacgccc
     9301 ggcttcgagg acgcccacct ccacctgatg agcggggcgc ggaacctcga gcgggtcgac
     9361 ctgtccgggg aggcgacgct cgcggggctg caggagagga tccgacgctt cgccgcggca
     9421 aacccgagga gcccgtgggt cgtcgggcgc ggctgggtct acggcacgtt cccgggcggc
     9481 ctgccgacga aggagctgct cgacgcggtc gtccccgatc ggccggccta catggacggc
     9541 tacgacggcc acaccggatg ggcgaacagc cgggcgctcg cgctcgccgg aatcacgaag
     9601 gagacgaagg acccggtgaa cggcaccatc gtccgcgacc cgaggtcggg tgagccgacg
     9661 ggcgccctga aggaggccgc cggcgacctc gtcgaggacc tcgtccccga gcccgacgcg
     9721 gagcggcact acgcgctcct gctgaatgcg ctcgggatcc tgaaccgcgg cgggatcacg
     9781 gcgatccagg acgcgggatg gacgaaggac gaggcagcgc ggttccttcc tctcctcgag
     9841 agggcgcgga aggaagggaa gctgacggtc cgcgcgcggc tcaccgtcga cctcgacccc
     9901 gccgagcccg cgccggggat cgcggaggcc gtccgccttc gggggctgta tcgagacggg
     9961 ccgatcgtct tcggcggcgt gaagggatac gtcgacggcg tgatcgaggc gcacaccgcc
    10021 gcgatgctcg cgccctattc cgacgacacg actctcggcg ccgggacgcc gcgatgggac
    10081 ggggccgcgc tcgagagcgc cgtcgccgcg gcggaccgga gggggctcca ggtctggctc
    10141 cacgcgatcg gcgaccgcgg catccggatg gcgctcgacg cgtacgaggc cgcggcgaag
    10201 gcgaacggcc ctcgcgaccg gcggcaccgc gtcgagcacg tcgaggccgt ctccgcggcc
    10261 gacgcgccgc gcttcggggc cctcggcgtc gtcgccgcca tgcagcctct ccacgcgaac
    10321 cccgaccaga acaacgcgga cgtctggtcg aagaacgtcg gccccgagcg cgagagccgc
    10381 gggttcgcct ggaggctcgt cgaggggtcc ggcgggcgga tcgccttcgg gagcgactgg
    10441 cccgtcgtgg cgcccgacgt catccggggc gtctcctgcg ccgtcacgcg gaagacgccc
    10501 gaggggacgc ccgccggcgg ttggcagccg cacctcgcgc tgacgcccga gagcgcgctc
    10561 cggcactaca cgatcgacgc cgccttcgcc ggcttcgcgg agaaggagcg cggctcgatc
    10621 tccgtcggga agcgggcaga cctcgtcgtc ctctcccgcg acctcctctc cggcccgccc
    10681 gaggggatcc ttcagacccg tgccctcctg acgctcttcg gcgggaagcc cgtctggcgc
    10741 gacccatcgt tctgacggcg aggtccacgc cgtcgcggtc ttcgacccgg gcctcgggtc
    10801 cgggccgcgt cgagcacccg ttctcccggg ggagccgtcc tctccccgtc gcgcgggaca
    10861 cctcacgcca ggactcgcgc gaagaggcgg cggggccccg gtcccggcgg tccctcgacg
    10921 cggcgggccg ttccgtatca gactccgccc ggatgcctcc gccgcttccg atccccgagt
    10981 gctactgggt cgtcccgggg ctcctcctgg cgggcgagta cccgttctcg ctcgtggagg
    11041 acgacgggcg ggagcgtctt cgggcgttcc tcgacgctgg catccgcctt ttcgtcgacc
    11101 tcaccgaggt ccgcgagagc ggacgcctcg gggcgctcga gccgtacgcc gagaccctgc
    11161 tcgaagaggc cgcggcccgt ggggtgtcgg tgcgggtcgt gcgccacgcg gtgagggaca
    11221 tgagcgtgcc gcgggacggg accctggagc ggatcgtcga ggagctggag ggggcggtcg
    11281 cgcgccggga gccggcctac gtccactgct ggggaggcat cggccggacc ggcacggcgg
    11341 tggccgcgtt tctctcccgc cggctcggcc tgaccggaga cgaggccctc gaccttctcg
    11401 acgcgctctg gcaggaggtt ccgaagagcg ccgtcttccc cgaatcgccc cagacggacg
    11461 agcagcgccg ggccgttcgc gcgttcgccg ggaggcgcgg ccccgcgggc ccgggcgagg
    11521 gcggatgagc tcccggatcg agtccccgac gaatccccgc gtcgccgcgg cctcgcgcgc
    11581 ggtgcgggag ggcgaggctc tcctcctcga gggggcgagg accctgcgcg acgcgctcga
    11641 cgccggcgtc gtgcccgacg tcgtcttcgc cgcccccgac ctcgctccga tcgacgcggc
    11701 ggtcgccgag gacgcccgcg cggcgggagc ctccgtggtc gaggtctccc agcgcgtcct
    11761 cgctcgcctc tcggagctcg gctcgacgcg cgcgctcgtc gcgctcgcgc ccgtcccgtc
    11821 cgcccggctc gagtcgctct cgcgcgccgc gctcgtcctc ctcctcgacg gcctccagga
    11881 cccggcgaac gtcggcgcga tcctccgcgc ggccgaggcg ttcggcgcgg gcggcgtgat
    11941 cctgaccccg gggacggcct ccccgtacgc gccgaaggcg ttccgcgcct cgtccggcag
    12001 cgccctccgg gtcccggtcg tccggaacgt cctgtcgacc gacgcggtgc gatgggtccg
    12061 ctcgaggggc gccctcctca tcggagccga tgcgcacggc ggcgaggagc cgacagccct
    12121 cgccggcgtc gcgccgctcg tcctcgcgat cggctccgag gggcacggcc tctcggcgcc
    12181 gctcgtcgcc gctctcgaga ggcgcgtgac gatcccgctt tcgccgcgag tcgagtcgct
    12241 caacgcggcg gtcgcgaccg ggatcctcct ctacgcgctc tccccgaaga cctccgcgga
    12301 gaagcgatag agcgccgcgg gagggctccc ctccttccag gaatcctcgg ggagacccgc
    12361 cttcaggcag acctgcgcga ggagggtcgg gacgtcccag ccccactccg gggcgacctg
    12421 cggcagcagg aggccggagc gcgagccctt cctcacgacg acgccgtgga ccccgatctc
    12481 gagctgccgg agggggtcgg agacctcgtc gagcgggctc aggacggaga cctcgaccga
    12541 gagccaccgc aggtcccgcg cctgggccgg cgcgaagcgc gggtcgcgca cgagcgccgc
    12601 gacggcattc gtctcgacgt cctcggcgag cgggcgccac ggctcgacgc tcccgatgca
    12661 gccgcggagc cggccgtccc gtctccaggt gacgaacgac gcgcccggct cggcgaggcg
    12721 cccggccggc ggcggcagct cgcgctttcc ctccgggagg ccgagggccc gcgcggcggc
    12781 gtgccgcgcc cgctggatca gatcgtccct ttcggcgtcg gtgagcatcg tctggcccct
    12841 ttccgtcacg ccggaacgac cggcgcgccg aagcccgcct cggcgaggag gcggaccgcc
    12901 gccgcctcga acccctcgag gtggcccgtg aagaagtggt ccgcccccgg aacggacgcg
    12961 gcccggaagc cgtggcgctc cgccgtctcg agggccagcg cgccggcgcc gaacgagtcc
    13021 tccccgccct ggacccagac gacgttgcgg ccctcgagcg cgcgcgcctc ctcctcgtcc
    13081 caacggtcca gcggcacccc gatcaggagg aggacgtgcg gggcgaccga gacggccgcc
    13141 cgcgccgcgc agagcgaccc gaacgagaaa ccggcgaggg cgagcggccc ggacggctgt
    13201 cgttcccgga cccagtgcac ggcggcgacg aggtcgtcgg tctcgccgcg cccctcgtcg
    13261 tagctccccg ccgaggctcc gactccccgg aagttgaagc ggagggcgga gaggtggagc
    13321 cggtccgaga agaccttcgc ggcgcggtgg acgaccttcg tgtgcatcgt cccgccgaag
    13381 aggggatgtg ggtgcgccac gacggcggcc ccgcgcggcc ggaccggaac gcgcaggagc
    13441 gcctcgaggt ccccggcggg cccgggcagc gtcagggact gcattcggac ggtgctcacg
    13501 gcgttcccga agttagcatg cgcggagtga acgaagtgac cgaggggacc gggaccgccc
    13561 tggcccttcc cgcgccgtcg ccgcggctgg agcgcgcgat cgtcctcgcg ctcgtcgtcc
    13621 tgtcggccgc cctggcgctc tggcatctgg cgggcgacgc cgcgacggcc gacgagccgg
    13681 ttcacgtcgc cgcggccgtc gagatcgcgc gcgaggggac cggacgctgg aacccggagc
    13741 acccgccgct cgccaaggcg ctcgccggcc tctcgctcgc gggccttccg ctccgcccgg
    13801 ccgacgaccc gctccgctcc gccgccggtc ccgctcgcct cctccggttc ctcttcgcga
    13861 acgagacgcc ggccgagacg atcctcttcc gggcccggct ccccttcgtc gcgctgctcg
    13921 cggcgctcct cctcgccgtg cggggcgagg cgcggaggcg atgggggccg tgggcgggaa
    13981 tcacggccct cgccttcgcc gcgctcgagc cgaacctgaa cgcccacgcc ggggtcgtcc
    14041 acaccgacct cgcggtcacg ctcttcgtcg tcctctcgct ctcgcccctc gcgcgcctcg
    14101 cccggcccga ggcgaggcgg gccacggccc tcctcggcct cctctggggg ctcgcgttcc
    14161 tctcgaagta cagcgcgccg ctcctcgccc tcgcgacgct cccgttcctc gccgccgacg
    14221 cgacgccgag gcggcaggac ttcgggcgcc tcctccgccg cctcgcggcg gccgcgtcga
    14281 tcgcgctggc cgtgacgctc gccggcttcg cgtgggccgg tcgcagccag acgcccgagg
    14341 accggcgggc gatcgccgtc gagaggctcg agctgaaggg gcgctcgccg gccgcggccc
    14401 ggctcgcgat ccgcgccggt gacctcctgc cgccgctcgg gaacgtcctg acgggagcgc
    14461 tctccgtcgc gctccagagc gaggtcggcg ccggcgtgaa ctacctcctc ggcaccgtct
    14521 cgcgcgaagg ctcaccctgg tacttccccc tcgccctcgc ggtcaagacg cccctcggcc
    14581 tcctcctcgg cctcggcgtc gcgctctccg cgcgcccggg ccgccgcctc gccgcggtgg
    14641 ccggcaccgc gctcctcctg ttcctcctcc tctcctctcg gacgacgtac aacatcggcg
    14701 tccgacacgc gctcctcgcc ttcccgctct tcgcgatcac cgcgggcgcc gccgccgcgg
    14761 gccgggcgga cccggcggac cggcgatccg cgagcctgcg gcgcggagcc gcggtcggcg
    14821 cgctcctcgt cgccgcgctc gaggcggcgg tggcccaccc gcacccgttc tcgttcttca
    14881 acgccctcgc gggaggagcc gaagggggcc ggcgcttctt cgccgacagc agcctcgact
    14941 ggggacagga cctcctccgg ctgaaggccg tcgcccccgc gctcgcaccc gacggcctcg
    15001 cgaccatcgt cttcgggggc gacctcccct cgcgccacgc gccgctcctc cgcccgccgc
    15061 ggcccggcga ggaagaccga cccggcgcga tcctcgcgat cggcgaggcg cctttcgcgc
    15121 tcggcccgga gctgcttcgg gcgaaggggg cgaccggaga cgccgaccgg ctggagtcga
    15181 tccggacggc cttgcgcgag cgtggccgac gcatcgggac cgtcggcggg tcgatcggag
    15241 tctggcggat cgagagcgct cccccgcgct gatcccgccg gctacggcct cccggccggc
    15301 ccgtcgaccc aggagagctc gcggaggatc acctgcccgt tcaggcgcgg ccggaactcg
    15361 agcctcgcgt cgaccccgta ggtccttcgg cggttgaaga gcgggatcag cgggagatcc
    15421 tcgaggacga cgcgcatcgc ctcgtgcaac gcgtcgagcc tccgcgccgg ggagaggatc
    15481 gccccggccc gttcgaccgc ttcgtcgagc cgcgggttgg cgtagccgcc gaagttcgag
    15541 actccccagc tccggccctg ccgcgtgtgg aggagggcgt cgaagagatg cgacgcgtcg
    15601 ccgttgcacg accagccgac cgagaagaag gggctctctc cccgctcgag acgagccgag
    15661 aggatgtgcc acgggaggct ggcgaccgtc gtccggatgc cgacccgcgc gagctgcgcg
    15721 gcgaggactc tcccgacgct cggggcgaat gcctccgcca tgtcgagcgt cacgtcgaac
    15781 ccttcaccca gccccgcccg acgcagcagc gcccgggccc gatccgggtc cggccgaggc
    15841 tcgccgccgt cggggaggtg gccgaagacc gaaggcggaa cgacctggtc gacggggacc
    15901 gcctcgaccc ccgaaaggtc gacgagctcc tgcgggtcga tggcgaggta gatcgcccgg
    15961 cgcacgtcgc gggagcggag cggaccgggc acgtcgaacc gcgtcccgag gaggacgacg
    16021 acgagccccg ggtggctcac gaaccggacg ttcttccccg cgccgtccag gtccttccgc
    16081 ttgagctccc gcgcgaggtg gagctccccc gccgcgagcg cggcggcccg cgcggtcgcg
    16141 tcgggaagga cccggaagcg cgcctcccgg atcggcggcg cgccgtcccg gtagcgctcg
    16201 ttcgccacga ggcggagctc cttccccggc acccacgaga cgaatcggta ggccgccgtc
    16261 ccgaccggaa cggtgacctc ctcgggcgtc ccggccggga cgattccgac ccccgcgagc
    16321 ttgttcagca ggaccggctc cggcaccgac gtgccgatct cgagcgtctc gtcgttgacg
    16381 gcctcgatcc tctcgagcga ctcgaggtgc ggcgcggccc ggaaacggcg ggcgcgctcg
    16441 aggctgaact tcgcgtctcg cgccgtcagc ggaaggccgt cgtggaagcg cacgcgagga
    16501 cgtagacgga aacgccacgt caggtcgttc gggttgctcc acccctcggc gagcgccggg
    16561 acgacgcgca tctgcgggtc gaacgtgacg agcggctcgt agacgttcgc gaggaccgag
    16621 tgggtgacgc tgtcgtcgtg gcgatgcgga tcgagcgtcc gcacgtcctg ctccatcgcg
    16681 accaggagcg cgggcggagg gggctgcgcg ccgcacgcgc cgagcgcgac cgaaaggagg
    16741 acgacggccc gggccgaccg acgcaccatg cccgattcta gtgcgcggac cggccggaac
    16801 gcggcacgtc ccgcagaccc ggacgacagc tcacgccttc agcgagccgt ccccggcccg
    16861 taccccgtga tcacgtacga gccgctcttg gcgtcccggc ggagcgtgat gacccggtgc
    16921 ttctgggccg actcgaggag gtcggagaag gcgcggaacc cgtagtacga ctcggagaag
    16981 ctcgggttct tgcgctgcat cgtctgcttg accatcgagc cccagaggat ctccttgttc
    17041 tcgcgctgga gcgcctgcat cgactcgatg aggagcgaga agacctctcg gctcttctcc
    17101 gggagccccg cgacggccgg gaccttcacc tgctcgcgga cgaggtcctc gtagtagatg
    17161 aactcgtcgc agttctcgat caggagctcg gacgtcgagc ccttcacgcc gaagccgagg
    17221 acggtcttgt tgttctcctt cagcttcgag acgaggggcg agaagtccga gtcgcccgag
    17281 ccgatcacga aggtcgtgat gtgctccttg ttcgaggcca ggtccatcgc gtcgacgacc
    17341 atccggatgt cggccgagtt cttcccggtc atcttgtgct gcggtatgtc gatcagctcg
    17401 aaggccgact cgtggaaggc ccgcttgtac tccgcgtacc gggaccagtc ggcgtaggcc
    17461 ttcttgatga cgatcttccc cttgtcgagg aggcgcgaga ggagcagctt gacgtcgaac
    17521 ttcgggtgcc cgctctccct gaggccgatg atgacgttgt cgatgtcgat gaagagggcg
    17581 agcttctggt cttcctggct catgctcact gggggtgcgg cggggacgac ggtctcgacg
    17641 atcgtcgtcg tggcgggaga gaaggcctcg gtctcgaaga acgtgacgtc ggcggcgggg
    17701 gtggcggccg cctccgcggc ggccgtggcg cccgacttcg tcgaccggcg acggcggcgc
    17761 gtgggacggg ttttctggct ggcttcgttc aagcgtctca tattacgaca catgcgagaa
    17821 tcgaacggat gccgatcagc gattcatcga acgaaggagt cgaggccctc cgcgcggcgg
    17881 tcgtggccgt cgacgtgacc ggacggggtg ccctcgcggt ctcgggtccc gatgccgcgg
    17941 agctcctcca cggcctcgtc acgaacgagg tgaaggccct gccccccggg tcgggctgcc
    18001 acgcggcgct cctcacgccg aaggggagga tgcgggccga gctcgccgtc ctccgcgtct
    18061 cggagtccga ggtcctcctc gactgcgacc cctccctcgc cgaaccgctc ctcggaatcc
    18121 tccagggcta cgtcccgttc agccggtcgg tccttcggga ccaaacgagc gagaccggcg
    18181 tcgtccacct cgagggcccg accgcggccg gggtcctgct cgccgcggcc ctgcccgagc
    18241 ccgcgccgga cgactacgcg catgccgccg gctcgctcga cggcgtcccg gtccgcgtcg
    18301 tccgcgtctc gcgcgccggc gagccgggct tcgacgttcg cacctcaccg accgccgtcc
    18361 ccgcgctcct cgacctcctc tcctcccgcg gggccgcact cgtctccccc gacgtcctcg
    18421 aagcgggccg gatcgaggcc ggcctccccc gctggggcgc cgagctgacg gagacgacgc
    18481 tcccgaacga ggccggcctc gagcggaccg cgatctccta tacgaagggg tgctacctcg
    18541 gacaggagac ggtcgcgcgg atccggacct acggccacgt gaaccggcgg ctcgtcgcgc
    18601 tcctcctgcc gatcggcagc gccgccgccc cgggcgacga cgtgcgcgcc ggcgaagaga
    18661 ccgttggaaa cgtgacgagc gcggtcgatt cctcccggcg cgcagcgcgg gtcgctctcg
    18721 cctacgtgaa gaaggggcac gacgagcccg ggacggcgct cgccgtggcg acgaaggagg
    18781 gcgcggccgc gggacgcgtg gccgccgtcc cgcttgggcg ctgaaactcg cgcccgcgag
    18841 ccgggcgccg ctccctacgt cgcgctcctc ttcgtccagc tcttcttcgg gacgctcccc
    18901 gtcttcggca agatcgccat gcgggagatg gagccgttcg cgctcgcggc cttccgtgcc
    18961 ctcttcgggg ccctcttcct ctcgctcgcg gcccgagcct tctcgggccc cgagccgcgc
    19021 cagacgctcc gggagaaggg agaggtggcg ctcctctcgc tcctcggcgt cgtggcgaac
    19081 caggtcctct tcatcaccgg cctctcgctc acgaccgcca cccacgccac gctcctcgtg
    19141 gcgaccgtcc ccgtcttcac gatcctcgtc gggctcctct cgcggaggga gcgcccctcg
    19201 ccgcggcggc tcctcgccgt cccgatcgcg ttcggcggcg tcctcttcct cctcgacccc
    19261 gcgaacctct cgttccgcca cgggacgttc gtcggcgacc tcctcgtcac gctcaactcc
    19321 gtcgcctacg cctggttcct cgtccgggcg cgcgacgtcc tcgtccgccg ccccgccctc
    19381 tcgttcgtgt cccaggcgtt ccggtacggg gccctgccga tcctcctcct cgccctcccg
    19441 gggctctcgc gcctccgccc cgccgagctc tccggtccgg ccctcggatc gctcgtcggc
    19501 gtcgtcctcc tcgggacggt cggctcctac gccctgaacg cctgggccct cgcccggacg
    19561 agcgcctcga cgacggcgtt cttcatctac gtccagccgg tcttcgcggg agcgttcgcc
    19621 ttcttcgcgc tcggcgagga gccctcgccc cggatcttcg gcgccgccgc cctcatcttc
    19681 gccggcgtcg ccctcgcgat ctggcccgcc cggaagcccc cggcctcctg acccggcccg
    19741 gaatcctcct tgccccatga cccgcgtcat acgcgggc
//
LOCUS       DAOONL010000066        19371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2848_length_19371_cov_22.226651, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000066 DAOONL010000000
VERSION     DAOONL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_19371_cov_22.226651"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLP50_10755"
     CDS             <1..449
                     /locus_tag="PLP50_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA52069.1"
                     /translation="GRLFDAVLLHDAVSHMTTRGDVAAAVATAFAHTAPGGVALFQPD
                     CVAETFAEETGTGGSDGGGRGLRYLEWRFRPEPEAERFVVEMAYLLREAGGEVRVAQD
                     RLEMGLFPRAYWLETLAAAGFEALVVPLEHSAAGDYEVFVGRRPVR"
     gene            complement(481..1623)
                     /locus_tag="PLP50_10760"
     CDS             complement(481..1623)
                     /locus_tag="PLP50_10760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA52070.1"
                     /translation="MTTTTLETTTTATDWVGRASQIGETLAPGVAGRDRLGEIDLAAF
                     EAFRAEGVTAALVPAEAGGGGASHADAGRLLRELGRHDGATAVALSMHMHLVAAQVWR
                     HRHGLDASALFRKVVERRAVLVSTGAADWIGSSGSARKVDGGYRVRARKSPASGCEAG
                     NVLVTSARWEEAPGGPRVLHFAVPMTAEGVRIEKTWDTLGLRATGSHTVVLEEVFVPD
                     AAVSLERPADVWHPVWNVILGAAMPLILSAYVGIADAAVARALEAAKGRAESHVLQLL
                     GEMLNAHTTAADAVDAMFRDADDLRFEATDSFAGRALSRKTVAADALIATVRLAIEAT
                     GGSGYTRSSDLERLYRDVHGVLFHPLPRARQTTFSGRAALGLAPVG"
     gene            1722..2396
                     /locus_tag="PLP50_10765"
     CDS             1722..2396
                     /locus_tag="PLP50_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPA52071.1"
                     /translation="MSERRSGYGQYCPISRALDLLGERWTLLVVRDLLVGTTRFNELA
                     RGLPGLSRSLLSLRLRQLERAGLLERVDGEYLLTAAGRELRPIVFALGEWGARWTFGE
                     PRPAELDPALLVWWMHTRLDTTGFPGRRQVFHVRFSDDERRFWIVVEPGGPSVCDIDP
                     GFETDVTITSDRASLYQVWLGRLPLEEAVRSGRVSFAGPVALTRRMTSVLRLSPMAEA
                     ARPATR"
     gene            2427..3401
                     /locus_tag="PLP50_10770"
     CDS             2427..3401
                     /locus_tag="PLP50_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPA52072.1"
                     /translation="MTTRPRGRTFLLALALFAPGALPAAPPGPSTFSIAAADPATGEV
                     GVAVASRFFAVGTVVPWAKANVGAVATQASANTSFGPDGLDLMARGLTAGEALKVLLR
                     GDEGRERRQVGLVTASGDAATFTGSGANAWAGGRKGPNYAVQGNILVGEAVVEAMERS
                     FLASAGRPLAERLLAALAAGDAAGGDSRGRQSAALLVCRAKGGYNGFSDRAVDVRVDD
                     HADPFRELARLVGMAVVNDLWNRGWTGFMERRHAEALKWQEEATARAEAQPAMLPEVV
                     YDLAVIRLAAGDRDGALAAARRALALNPKLAAQAAVDPDLEAIRAELR"
     gene            3423..4157
                     /locus_tag="PLP50_10775"
     CDS             3423..4157
                     /locus_tag="PLP50_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52073.1"
                     /translation="MNVDRRLLWTLAGVAGLSLLAVAYLLGRASGTERTEPRPEGAAP
                     AFSPVLAGTGANVVMTPAPTSPSADPAIGELPFSSAEHSSPDALPESRTPFLPTPDPE
                     PGLAPPPEDPERAAVAAYLDAVDAIQASGLAGSPENAANEMAVALARGDASGLDGLVR
                     EAEEARRRLLGISPPAACAAHHRETLASFDDALEMLRALRDASASGDPAASLAAVAAR
                     ATALRSRAEALQREDRVLRVRAGRVP"
     gene            4259..4687
                     /locus_tag="PLP50_10780"
     CDS             4259..4687
                     /locus_tag="PLP50_10780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPA52074.1"
                     /translation="MPSQERLDALRASEHPRCFLCSPESPAGLKLRFRVQPDGSVAAF
                     FPCREVFQSYPETLHGGIVAALLDAAMANALFSIGVVAVTAEMDVRYRAPVVLQRGAV
                     VRGVVEKTTSHPLYYVRGEVEQDRKVVARASARFLVRGVF"
     gene            4695..5144
                     /locus_tag="PLP50_10785"
     CDS             4695..5144
                     /locus_tag="PLP50_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPA52075.1"
                     /translation="MPDTPESPAAPPQGPVLFYTPTLVDHFVNPRNVGELPDSETDGY
                     GLVGDPSCGDQMKLWIGVKFGRIARIAFKSYGCPGAIATSSMLTHLALGLTLEDARRL
                     TDDDVVEALGGIPEAKKHCSLLGVKALQAALDDRERREAERPETAKP"
     gene            5141..6301
                     /locus_tag="PLP50_10790"
     CDS             5141..6301
                     /locus_tag="PLP50_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPA52076.1"
                     /translation="MIYLDSNATTPLRPEALEAMLPFLSERQGNPSSPHRAGHEARAA
                     VEEARRRIAARLGFEPEEIVFCASGTEADNLALRGVTRALRKKGRHLVTSAIEHHAVL
                     HTARDLERDGWEVTSLPVGPDGAVRPEALAAALRDDTVLVTVMRANNDTGVVQPVAEL
                     AALCRERGVVFHTDAVQSAGKLAERPGDLGAGLVTVSAHKLYGPKGVAALCVRRGTPL
                     SPVTTGGGHERGLRAGTENVAGIVGFAAAVDLAFEALAADGPRLAALRDRLEARVLAR
                     VDGVRVNGAGAPRVPNTSSLSFRGVDGESIVLGLDVRGICVSTGSACSTGDPEPSHVL
                     LAMGLGPRAAQGSIRISLGRTTTEADVDATATALLETVTRLRAISSAPDAEG"
     gene            complement(6321..7676)
                     /locus_tag="PLP50_10795"
     CDS             complement(6321..7676)
                     /locus_tag="PLP50_10795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA52077.1"
                     /translation="MTTDGPAGRSPRNVAVAAIYLLALAWILGKAALFRERVPPGVPP
                     DEPVHVSYVAWLEASGAVLPRYEEMRLLEPNGRFGAQPSYLAHPPAYYHVLRGLARVT
                     GGVPSGPLDAATRRLRSLSAPLFAAAAALVLWLGFRRAAPLPAHALYAATVATVPPFA
                     FVGSAVNNDVLAFLAGGLALLGLARLLEGRAGATTGTLVGAGLALALLSKATAGLLVL
                     LAAAAALALARRTVRWERDPRFLLALLPWLLLPAIHYVPILVRYGTPVPSLDVVDPEA
                     YARSAFVEAPGTPRLGLFPWALKLLKVFGSTWLSLIAHVWLPVGPLPTLAGPALAALL
                     AALGLLRRRRAVGTDDAPLDDLVRAGAVALAGTLLVNLAWARDGYLATERLGGIHARY
                     YLPLFPVLALAAARGLERLPARGLERVPARALLAGAVVLLLVLFDAGVTARYLALFPA
                     P"
     gene            complement(7682..8533)
                     /locus_tag="PLP50_10800"
     CDS             complement(7682..8533)
                     /locus_tag="PLP50_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPA52078.1"
                     /translation="MISRRFTLASRLGEPIHGDLRLPDGPGPHPVVVCCHGFKGFKDW
                     GFHPWLGARLADAGLAAVHFNFSRNGVREADGDIEDLDAFRRNTLSIERDDLDTVIDA
                     VLTGRLDLSLDGARLGLLGHSRGGGLALLGASERPEAKALATWAAVSHFDRIADEPTL
                     AEWRRTGVYEVVNSRTGQKLPMGVAFLDDVLANLARLDLLAAARRLAVPWLLVHGTAD
                     LTVELGEAEELHEATAGRARLFPLEGAGHTFGAVHPFAGPTPHLAAAAEATLAHFRTS
                     LAGASAA"
     gene            complement(8548..9708)
                     /locus_tag="PLP50_10805"
     CDS             complement(8548..9708)
                     /locus_tag="PLP50_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPA52079.1"
                     /translation="MPLLPLSPIDHVFTGRGAYPIEFVFAYEGAIDAGRLEASLREAI
                     EAFPPVGSRFVRLDERSLAFEPCADGCAFTVASSPSDWDATPRKDVFLDPVSGKEGEP
                     LGRALLTRTPSGCVLGFSLSHAVADGYSYFHFLTSWARLFRGEPILPPTHDRAALAAG
                     LDGAKSGGAADDAGLFRSGPRPDVDRAHLAIHRHVLSRAELVARHAEAQEGNPLRLSH
                     NDTAAAWLWKTRLAEWSGDDPSLETYVSCPVDFRRLLPSTGPAYFGNAVTLAKASIPK
                     GELGAASLGDLATRVRRAVDAVDVVAARRSLASLEALRRSEGLGAMEECHVVHPSQGL
                     LLTNLSRLPVAEVAFDAGAPVAFEILTPGVRGAVALPAPDGGLDLRVFLPSA"
     gene            complement(9768..10517)
                     /locus_tag="PLP50_10810"
     CDS             complement(9768..10517)
                     /locus_tag="PLP50_10810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPA52080.1"
                     /translation="MSGRTIAIGDVHGDLEGLERLLARLPVLLPEDTLLFVGDYVDRG
                     PNPAGVVRRVRSLPSETAARVVFLRGNHEDAWLKACREGAPEFVLPRGNGCYSTYRSY
                     TGGPVPGPGENPTADELRAMATGAFLPADVVAWMGGLPHFHEDEHAIYVHAGLPHDGR
                     RWLHPSEVADPRPLLWQRSRSFYEGYKGKRVVFGHTPVRKLPQDQSVHTPGDPLDVYV
                     TASLAGIDTGAGMGGFLSAIELPEMRVLDAR"
     gene            10686..11729
                     /locus_tag="PLP50_10815"
     CDS             10686..11729
                     /locus_tag="PLP50_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPA52081.1"
                     /translation="MRARTGLLLLALPLALLLVPAGSLADDDPGVAARLKALEERIES
                     LEFDLDQVRKSSDDALFWLRLGDVAEIDKVNVAGPPNPRAKDRYGIANERHPIRFAQY
                     VFAPKVREAGRKYPVVVLPHGGVHGDFGTYHVHIVKEMLEQGWVVLAPEYRGSTGYGK
                     GFYEAIDYGGLEVDDVIAGLDWAVSELPYVDGSRAAIAGWSHGGLIALLAVMHHPEKF
                     RAAYAGVPVTDLVTRLGYHEQSYEDLFAAKHHVGKKVVEDVDEYRRRSPVFWASKLKT
                     PLLVHATTNDRDVNVIEAENLIAALKAEGKSFEHRIYTDAPAGHSFNRIDTRLAKESR
                     KEIWAFLRKHLGR"
     gene            11775..12188
                     /locus_tag="PLP50_10820"
     CDS             11775..12188
                     /locus_tag="PLP50_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HPA52082.1"
                     /translation="MAGLGISVPGFLLRLLAAALLVFATYNPAGWSFLHWVRKSGGGP
                     LPLKVLAGIALVAGWVFCVRATLRSLGHVGTALAAAFFGVLIWLFVDLRGTDVEGSTL
                     AWIVLACLAATLAVGLSFSLVRRRVTGQIDDTDEE"
     gene            12196..13221
                     /locus_tag="PLP50_10825"
     CDS             12196..13221
                     /locus_tag="PLP50_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPA52083.1"
                     /translation="MSRSVLRAEIGEQPDVLTRLLVREGPRIRRIARDLARRDLRFVL
                     VAARGTSDNAARYAQYALGLTCRLPVALAGPSLVTLYDSPPRLKGALVVGISQSGRSP
                     DVVETIASARREGVPTLAIVNDPESPLAKAAAEVIPLQAGVERSVAATKTYTAQLLAV
                     ALLAAGLSGSRKHAEGLGALPDAVADALGTEGAARRAAARLATAARLVVLSRGLNYPT
                     AHELALKLKELALLEAEALSGADFRHGPIALAGPKLPAIVVSPPGTATEEDLSDLAAE
                     LRRRGSPVLAIGPHGRGSLPVPRLPELLSPIVAIVPGQLLALHAARARGLDPDAPRGL
                     SKVTETR"
     gene            13292..13903
                     /locus_tag="PLP50_10830"
     CDS             13292..13903
                     /locus_tag="PLP50_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52084.1"
                     /translation="MKKTFALVACALLVAGLAACNADKGPADAAIKAADQAITAAAPE
                     AEKFIPEQLTAAKDALKAAQDQFAKGDYKAALAAATELGTKAQELATAAAAKKDELTK
                     AWTDLSGSLPQMVTAIQAKVEELGKAKKLPKGMDKAALTGAQEGLAGIQKSWEEASAA
                     FTGGNLVDAMAKAGGLKEKATEIMSALGLQAPAPAEAPAAPAN"
     gene            complement(14015..14764)
                     /locus_tag="PLP50_10835"
     CDS             complement(14015..14764)
                     /locus_tag="PLP50_10835"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPA52085.1"
                     /translation="MRTTDLNADVGELPDLATVEEELLGVVTSVNVACGGHAGDEASM
                     ERVVRAALARGVAIGAHPSYPDRAGFGRRGMSLPGGLLAGTVAEQLASLLEVAARCGA
                     RLSHVKPHGALYNAAAKDPDLARAFAEGVARVAPGVVLVGLAGSEMLEAFAGAGFRVA
                     GEAFADRGYEPDGTLIPRSRAGAMKETPEDAARQALSIVLDGQVVAAGGARVRLDAQT
                     ICLHSDTPGAPAFARAIAERLRAAGVVLRAL"
     gene            complement(14761..15987)
                     /locus_tag="PLP50_10840"
     CDS             complement(14761..15987)
                     /locus_tag="PLP50_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA52086.1"
                     /translation="MRLAWLREASPEARRALLAAASGWMLDAMDVMLYAFALTAIRAE
                     FGLDGARAGLLASVTLVASAVGGIGAGLLADRFGRARVLVLSILAYALFTGLTATSRT
                     VLELALWRTLVGLGLGAEWSAGSVLVAETWPAAHRGKAIGLMQSGWAVGYVLAAFLAA
                     AVLPAWGWRPLFVLGVLPGLLAVWVRRRVAEPAIWRERGALAGGGTGLALLLKPPLLG
                     RAVAATSLATVLLFAYWGLFTWVPTFLAAPAAQGGAGLGLVRSTGFVVAMQAGAFLGY
                     TTFGFLSDRFGRRPVFLAFVLGAAVVVPAFGLLARSEAALLLLAPAVGYLGHGYFSVF
                     GVLLAELFPSSVRGAAQGFCYNAGRAVSALAPFSIGALADRHGLGSALALTSALYLAG
                     ALLMRLLPETRGRELE"
     gene            complement(15984..16916)
                     /locus_tag="PLP50_10845"
     CDS             complement(15984..16916)
                     /locus_tag="PLP50_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HPA52087.1"
                     /translation="MIRVLSPGLLTTVQDLGRPGLAGLGVPAGGAADAHALRLGNLLL
                     GNEEGAAALEATLVGPELLFEEAALVVLAGAPFGARLDGAPLPPWKTVEVPAGGRLAV
                     GRAHSGARACVCVRGGLDLPPVLGSRATDVATGFGGLDGRPLGAGDRLSVGPARGAPK
                     GRRVDAAAARWSGPRRRLRVTPGPQGDWFGEDVVEAFFSRSFRVSASSSRTGVRLGGE
                     ALTAPARSLVTEGVVAGAVQIPPGGLPIVLFVEHPSTGGYPKIGSVISADLSALAQLR
                     PGEEVRFARVSFEEARRLLLDREARLDAPGVFLS"
     gene            complement(16913..17620)
                     /gene="pxpB"
                     /locus_tag="PLP50_10850"
     CDS             complement(16913..17620)
                     /gene="pxpB"
                     /locus_tag="PLP50_10850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPA52088.1"
                     /translation="MTKDAPAGLPRLLPASDAALLVVLGEGPSEAATAAVLALRAALA
                     ARPPRGLLDLRPAYASLLVAFDPRATSHAELSRAVEPLLPPPGLPFAPAARCVEVPVC
                     YEGDCAPDLADVARGAGLSPEEAAELHAAAPYRVAFLGFSPGFAYLLGLPPLLATPRL
                     PAPRLAVPAGSVGIAGGQTGLYPRPTPGGWRLVGRTPLALFEPAREPAALLAPGDAVR
                     LVPIPRRDFDRLRREKP"
     gene            17703..18128
                     /locus_tag="PLP50_10855"
     CDS             17703..18128
                     /locus_tag="PLP50_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52089.1"
                     /translation="MALDVFHQVLAKLHEAAGGDPRQMVPFVEVLKQEKLFGSVDMIT
                     TRLQSEGWIADAPKKDHVFVTTWGVEELRRTSAASGAAKPAEPKNAAALRAAAERARE
                     LAATLEELASGTAGTAAQRKAKAKKALAAATEAVDEALG"
     gene            18125..>19371
                     /locus_tag="PLP50_10860"
     CDS             18125..>19371
                     /locus_tag="PLP50_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA52090.1"
                     /translation="MSLDALLPSPVRFLEGRLGRLRAPESLARYEAYFEERGKAISEA
                     VDRAGTPKLLMFDRFGTRIDEVVFPPAYHELVRRAYEAGVVFRAVEEGDLAASYQIGY
                     VTSFYDAGLYCPHTVSLSTAIPLAKYGSAELRAKYLGKLLKRDATVWQGATWMTEAKG
                     GSDLGAFVETVARPDGERWLLTGEKYFTSNVGAELAIAAARPEGAPPGVRGLELFLVP
                     RRRDDGALNFRVRRLKEKIGTRSVPTGEVELDGSEAYLLGARGQGVYLVLEALNVSRV
                     CNSIGAVALTQRALHEAWSFAEGRVAFGRPVAEQPLFARQARERHETLLDAFALAWEA
                     VTLLSEVWRETPRYSDRYHLFRLVAHLSKYWTAEVAVQSAKWAMEAHGGAGILSDYPV
                     ERLFREAIILGIWEGTPHRQVLDG"
BASE COUNT         2697 a         7129 c         7280 g         2265 t
ORIGIN      
        1 tcgggcgcct cttcgacgcc gtgctcctcc acgacgcggt ctcccacatg acgacccgcg
       61 gggacgtcgc ggccgcggtc gccacggcgt tcgcccacac cgctcccgga ggagtcgcct
      121 tgttccagcc cgactgcgtg gccgagacgt tcgccgagga gaccggcacg ggcgggagcg
      181 acggcggtgg gcggggcctg cggtacctcg agtggcgctt ccgccccgag ccggaggccg
      241 agcggttcgt cgtcgagatg gcctacctcc tgcgtgaggc aggcggcgag gtgcgtgtcg
      301 cgcaggaccg gctcgagatg gggctcttcc cgcgcgcgta ctggctcgaa acgctcgccg
      361 cggccggctt cgaggcgctc gtcgtgccgc tcgagcactc ggcggcgggg gactacgagg
      421 tcttcgtcgg gcggcgcccc gtgcgttagc gcgccggtcg cgtgcgggcc ggggcactcc
      481 tcacccgacc ggggcgaggc ccagggccgc cctcccgctg aaggtcgtct gccgcgcccg
      541 cgggagcggg tggaagagga cgccgtggac gtcgcggtag aggcgctcga ggtccgagct
      601 ccgcgtgtac ccgcttccgc ccgtcgcctc gatcgcgagt cgcacggtcg caatgagcgc
      661 gtcggcggcg accgtcttcc gcgagagggc gcggccggcg aaggagtcgg tcgcctcgaa
      721 ccggaggtcg tccgcgtcgc ggaacatcgc gtcgacggcg tcggcggccg tcgtgtgcgc
      781 gttgagcatc tcgccgagga gctggaggac gtgcgactcg gcgcgcccct tcgccgcctc
      841 gagcgcccgc gcgaccgcgg cgtcggcgat gccgacgtag gccgagagga tgagcggcat
      901 cgccgcgccg aggatgacgt tccagaccgg gtgccagacg tcggccggcc tctcgagcga
      961 gaccgcggcg tcggggacga agacctcctc gaggacgacg gtgtgcgagc ccgtggcgcg
     1021 aaggccgagc gtgtcccagg tcttttcgat gcggacccct tcagccgtca tcgggaccgc
     1081 gaagtggagg acccggggcc cgccgggcgc ctcctcccac cgggcgctcg tcacgaggac
     1141 gttccccgcc tcgcacccgc tcgccggcga cttacgggcg cgtacccggt agccaccgtc
     1201 gaccttccgc gcgcttccgc tcgagccgat ccagtcggcc gcgccggtgc tgacgaggac
     1261 ggccctcctc tcgacgacct tccggaagag cgcggaggcg tccaggccgt gccggtgccg
     1321 ccagacctgc gcggcgacga ggtgcatgtg catcgagaga gcgacggccg tggcgccgtc
     1381 gtggcggccg agctcacgga ggagccggcc ggcgtcagcg tgcgaggcgc ctccgccgcc
     1441 cgcctcggcc ggaacgagcg ccgcggtgac gccctccgcg cggaaggcct cgaacgccgc
     1501 gagatcgatc tcgccgaggc ggtcgcgccc ggcgacgccg ggggcgaggg tctcgccgat
     1561 ctggctggcg cgaccgaccc agtcggtcgc cgtggtcgtc gtctccagtg tcgtcgtcgt
     1621 catgccggaa ggatgggaac gctccggcct cgcgaccagt tcaaaaacga gaccggaccg
     1681 gtccagaatt cgcaccgaca cccggcccgg gaggtgacga cgtgagcgaa cgacggtccg
     1741 gctacggaca gtactgcccc atttcccgcg cgctcgacct cctcggcgag cggtggacgc
     1801 tcctcgtggt tcgcgacctg ctcgtcggca cgacccgctt caacgagctc gctcgcggct
     1861 tgccgggcct ctcgcggagc ctcctctcgc tccggctccg ccagctcgag cgcgccggcc
     1921 tcctggagcg ggtcgacggg gagtacctgc tcaccgcggc ggggcgcgag ctccggccga
     1981 tcgtcttcgc cctcggcgag tggggcgcgc gctggacgtt cggagagccg cgccccgcgg
     2041 agctcgaccc ggccctcctc gtctggtgga tgcacacgcg cctcgacacg acggggtttc
     2101 cggggcgccg ccaggtcttc cacgtccgct tctccgacga cgagcggcgc ttctggatcg
     2161 tcgtcgagcc cggcggccct tccgtgtgcg acatcgaccc gggcttcgag accgacgtga
     2221 cgatcacctc cgacagggcg tcgctctacc aggtctggct cgggcggctc ccgctcgagg
     2281 aagcggtccg ttcgggccgc gtgtcgttcg cggggcctgt cgcgctcacg cgccggatga
     2341 cgtcggtcct ccgtctgagc ccgatggccg aggccgcgcg gccggccacc cggtgaggcc
     2401 cgatagactc cccccggggc agacgcatga cgacgagacc tcgcggccgg acctttctcc
     2461 tcgcgctcgc gctcttcgcg cccggggcgc tcccggccgc cccgcccggc ccgtcgacgt
     2521 tctcgatcgc ggcggccgac ccggcgacgg gcgaggtcgg cgtcgccgtc gcgagccgct
     2581 tcttcgccgt cgggacggtc gtcccctggg cgaaggcgaa cgtcggggcc gtcgcgacgc
     2641 aggcctccgc gaacacctcg ttcgggccgg acggcctcga cctgatggcg cgcggcctga
     2701 cggccggcga ggcgctgaag gtcctcctcc gaggcgacga ggggcgcgag cggcgacagg
     2761 tcggcctcgt gacggcctcg ggcgacgcgg cgacgttcac cggctccggc gcgaacgcgt
     2821 gggccggcgg gcggaagggg ccgaactacg ccgtgcaggg gaacatcctc gtcggcgagg
     2881 cggtcgtcga ggcgatggag cgctcgttcc tcgcgagcgc cgggcggccg ctcgccgaac
     2941 gactcctcgc ggcgctcgcc gcgggcgacg cggccggcgg cgacagccgg ggtcggcagt
     3001 cagccgccct cctcgtctgc cgcgcgaagg ggggctacaa cggcttctcc gaccgcgccg
     3061 tcgacgtccg cgtggacgac cacgcggacc cgtttcgcga gctcgcgcgc ctcgtcggga
     3121 tggccgtcgt gaacgacctc tggaaccgcg gctggaccgg cttcatggag aggcgtcacg
     3181 ccgaggcgct gaagtggcag gaggaggcga ccgcccgtgc cgaggcgcaa ccggcgatgc
     3241 tccccgaggt cgtctacgac ctcgccgtca tccggctcgc cgcgggagac cgtgacggcg
     3301 ccctcgcggc tgcccggcgc gcgctcgcgc tgaacccgaa gctcgccgcg caggccgccg
     3361 tcgaccccga cctcgaggcg atccgcgcgg agctgcggta gggagcggaa cgagtggcga
     3421 cggtgaacgt cgaccggagg ctcctctgga ccctcgcggg cgtcgcgggg ctgtcgctcc
     3481 tcgcggtcgc gtacctcctc ggacgcgcga gcggcacgga gcggacggaa cctcgacccg
     3541 aaggagccgc cccggccttc tctccggttc tcgcggggac cggcgcgaac gtcgtcatga
     3601 cgcctgcccc gacgtcgccg agcgccgacc ccgcgatcgg agagctcccc ttctcctccg
     3661 cggagcactc ttcccccgac gcccttcccg agtcgcggac tccgttcctc ccgacgcccg
     3721 atcccgagcc cgggctcgcg ccgccgcccg aggacccgga gcgcgccgcg gtcgcggcct
     3781 acctcgacgc cgtcgacgcg atccaggcct ccggcctcgc ggggagcccg gagaacgccg
     3841 cgaacgagat ggcggtggcc ctcgcgcggg gggacgcgtc gggcctcgac gggctcgtcc
     3901 gcgaggcgga ggaggcgcgc cgccggctcc tcggcatctc gccgccggcg gcctgcgccg
     3961 cccaccaccg ggagaccctc gcgagcttcg acgacgcgct cgagatgctc cgcgcgctcc
     4021 gtgacgcgag cgcctcgggc gatcccgccg cctctctcgc cgcggtcgcg gcccgtgcga
     4081 cggccctccg ctcccgcgcc gaggccctgc agcgtgagga ccgcgtgctc cgcgtccgcg
     4141 ccggccgggt gccctgaccc gcgggcgcac cttcgtcggg cttgaccgaa acgaacatac
     4201 gcccagaata gggatcggga agagccgcag tcccttcatg ccagacccgt ccgaacccgt
     4261 gccgagccag gaacggctcg acgccctccg agcctccgag cacccgcgct gcttcctttg
     4321 cagtcccgag agccccgccg ggctgaagct gaggttccgg gtccagcccg acggctccgt
     4381 cgccgcgttc ttcccgtgcc gtgaggtctt ccagagctac ccggagacgc tccacggcgg
     4441 aatcgtcgcc gctctcctcg acgcggccat ggccaacgcg ctcttctcga tcggggtcgt
     4501 cgccgtgacc gcggagatgg acgttcgcta ccgcgcgccc gtcgtcctcc agcggggcgc
     4561 cgtcgtccgc ggcgtcgtcg agaagacgac gtcccatccc ctctactacg tccgtggcga
     4621 ggtggagcag gaccggaagg tcgtcgcccg cgcctcggcc cgattcctcg tccgcggcgt
     4681 cttctaggag agccatgccc gatacgcccg agtcccccgc cgcgcctccc caggggccgg
     4741 tcctcttcta cacgccgacg ctcgtcgacc acttcgtcaa cccgcgcaac gtcggagagc
     4801 ttcccgactc ggagaccgac ggctacggcc tcgtcggcga cccctcctgc ggcgaccaga
     4861 tgaagctctg gatcggcgtg aagttcggga ggatcgcccg gatcgcgttc aagtcatacg
     4921 gctgccccgg cgcgatcgcc acgagcagca tgctgacgca cctcgctctc ggcctgacgc
     4981 tcgaagacgc gcgacgcctc accgacgacg acgtcgtcga ggcgctcggc gggatccccg
     5041 aggcgaagaa gcactgctcg ctcctcggcg tcaaggcgct ccaggccgcc ctcgacgatc
     5101 gggagaggcg agaggcggag aggccggaga cggcgaagcc gtgatctacc tcgacagcaa
     5161 cgcgacgacg ccgcttcgac ccgaggccct cgaggcgatg ctcccgttcc tcagcgagag
     5221 gcaggggaat ccctccagcc cccaccgcgc cggccacgag gcgcgggccg ccgtcgagga
     5281 ggcgcgccgg cggatcgccg cgaggctcgg cttcgaaccg gaggagatcg tcttctgcgc
     5341 gagcggcacg gaggccgaca acctcgcgct tcggggcgtg acgcgcgcgc tccggaagaa
     5401 ggggcgccac ctcgtcacga gcgcgatcga gcaccacgcc gtcctccaca ccgcgcggga
     5461 cctcgagcgc gacggctggg aggtgacgag cctcccagtc ggccccgacg gcgccgtaag
     5521 gccggaggcg ctcgccgccg cgctccgcga cgacaccgtc ctcgtcacgg tgatgcgggc
     5581 gaacaacgac accggggtcg tccagccggt cgccgagctg gccgcgctct gccgcgagcg
     5641 cggcgtcgtc ttccacaccg acgccgtcca gtcggccgga aagctcgccg agcggcccgg
     5701 cgacctcggc gccggcctcg tcaccgtctc ggcccacaag ctctacggcc cgaagggggt
     5761 cgccgcgctg tgcgtgaggc ggggaacgcc gctttcgccc gtcacgaccg ggggcgggca
     5821 cgagaggggc cttcgcgccg gaacggagaa cgtcgccggg atcgtcggct tcgccgctgc
     5881 cgtcgacctc gcgttcgaag cgctcgccgc cgacggcccg cgtctcgccg cgctgcgcga
     5941 ccgcctcgag gcacgcgtcc tcgcccgggt cgacggcgtc cgggtgaacg gcgccggcgc
     6001 cccgcgcgtg ccgaacacct cgagcctctc cttccgcggc gtcgacggcg agtcgatcgt
     6061 cctcggcctc gacgtgaggg ggatttgcgt ctccaccggc tcggcctgct cgaccggaga
     6121 cccggagccg tcgcatgtcc tcctcgcgat gggcctcggc ccgcgcgccg cgcaggggtc
     6181 gatccggatc tctctcgggc ggacgacgac cgaggcggac gtcgacgcga cggccaccgc
     6241 cctcctcgag acggtcaccc gcctccgcgc catctcgagc gccccggacg cggaggggtg
     6301 acgactccgg gccgggaacg tcagggcgcc ggaaagagcg cgaggtagcg cgccgtcacc
     6361 cccgcgtcga agaggacgag gaggaggacg accgcgccgg cgaggagcgc acgcgcggga
     6421 acgcgctcga gcccgcgcgc cgggagacgc tcgagaccgc gcgccgccgc gagggcgagg
     6481 accgggaaga gcggcaggta gtagcgcgcg tgaatgcctc cgaggcgctc cgtggcgagg
     6541 tagccgtccc gcgcccacgc gaggttgacg aggagcgtcc cggcgagggc gacggcgccg
     6601 gcccggacga ggtcgtcgag cggggcgtcg tcggtgccga ccgcgcgcct cctgcgcagg
     6661 agcccgagcg ccgcgaggag cgcggcgagg gccggaccgg cgagcgtcgg gagcggtccg
     6721 accgggagcc agacgtgcgc gatgagcgag agccacgtcg agccgaagac cttcaggagc
     6781 ttcagggccc aggggaagag gccgaggcgc ggagtgcccg gcgcctcgac gaaggccgaa
     6841 cgggcgtacg cctccgggtc gacgacgtcc agcgaaggga ccggcgtgcc gtagcgtacg
     6901 aggatcggga cgtagtggat cgccgggagg aggagccacg ggaggagcgc gaggaggaag
     6961 cgggggtccc gctcccagcg gacggtgcgt cgcgcgaggg cgagcgccgc ggccgcggcg
     7021 aggaggacga ggagtccggc cgtggccttc gagaggagcg caagggcgag gcccgcgccg
     7081 acgagcgttc cggtcgtcgc gccggcgcgc ccctcgagga ggcgcgcgag gccgaggagc
     7141 gcgaggccgc cggcgaggaa cgcgaggacg tcgttgttca cggcggagcc gacgaaggcg
     7201 aacggcggga ccgtcgcgac cgtcgccgcg taaagggcgt gcgccgggag cggcgccgcg
     7261 cgccggaagc cgagccagag gacgagcgcg gcggccgccg cgaaaagcgg cgccgagaga
     7321 gagcggaggc ggcgcgtcgc cgcatcgaga ggcccggagg ggacccctcc cgtgacgcgc
     7381 gcgaggccgc ggaggacgtg gtagtaggcg ggcgggtgag cgaggtacga cggctgagcg
     7441 ccgaagcgcc cgttcggctc gaggaggcgc atctcctcgt agcgcgggag gacggccccg
     7501 gacgcctcga gccaggcgac gtaggagacg tggaccggct cgtcgggcgg gacgcccggc
     7561 gggacgcgct cgcggaagag ggccgccttc ccgaggatcc aggcgagggc aaggaggtag
     7621 atcgccgcga ccgcgacgtt cctgggggac cggcccgcgg gaccgtcggt cgtcacggcg
     7681 gtcaggccgc cgaggccccg gcgagcgagg tacggaagtg ggcgagggtc gcctcggcgg
     7741 ccgccgcgag gtggggcgtc ggcccggcga acgggtggac cgcgccgaac gtgtgccccg
     7801 ccccttcgag cgggaagagc cgggcgcgtc ccgccgtcgc ctcgtgaagc tcttccgcct
     7861 cgccgagctc caccgtcagg tcggccgtcc cgtggacgag gagccacgga acggcgaggc
     7921 gccgtgccgc cgcgagaagg tcgaggcggg cgaggttcgc gaggacgtcg tcgaggaacg
     7981 cgactcccat cgggagcttc tgcccggtgc gcgagttgac gacctcgtag acgccggtcc
     8041 gtcgccactc ggcgagagtc ggctcgtccg cgatccggtc gaagtgcgag acggcggccc
     8101 aggtcgcgag cgccttcgcc tcggggcgct ccgacgcgcc gaggagcgcg aggccgccgc
     8161 cgcggctgtg gccgaggagg ccgagccggg cgccgtcgag cgaaaggtcg aggcgcccgg
     8221 tgaggaccgc gtcgatcacc gtgtcgaggt cgtcgcgctc gatcgagagg gtgttccgcc
     8281 ggaaggcgtc gaggtcctcg atgtcgccgt ccgcctcgcg gacgccgttt cgcgagaagt
     8341 tgaagtggac cgccgcgagc cccgcgtccg cgagacgcgc gccgagccag gggtgaaagc
     8401 cccagtcctt gaatcccttg aagccgtgac agcagacgac gacggggtgc ggcccggggc
     8461 cgtcggggag gcggaggtcg ccgtggatcg gctcgccgag ccggctcgcg agggtgaagc
     8521 gacgggagat caccgcgcga ttgtggctca cgcggacggg aggaagacgc gcaggtcgag
     8581 gccgccgtcc ggagccggaa gcgcgacggc gccgcggacg ccgggcgtca ggatctcgaa
     8641 cgcgacgggc gctccggcgt cgaacgcgac ctcggcgacg gggaggcgcg agaggttcgt
     8701 caggaggagc ccctgcgagg ggtggacgac gtggcactcc tccatcgccc cgagcccctc
     8761 ggagcggcgg agcgcctcga gcgaggcgag cgaccggcgc gcggccacga cgtcgaccgc
     8821 gtccacggcg cggcggacgc gcgtcgcgag gtcgccgagc gaggccgcgc cgagctcccc
     8881 cttcgggatc gaagctttcg cgagggtgac cgcgttgccg aagtaggctg ggccggtcga
     8941 cgggaggagg cgccggaagt cgaccgggca gctgacgtac gtctcgagcg acgggtcgtc
     9001 gccggaccac tccgcgagtc gcgtcttcca gagccaggcg gcggcggtgt cgttgtgcga
     9061 gaggcgcagg gggttcccct cctgcgcctc cgcgtgccgc gcgacgagct cggcccgcga
     9121 caggacgtga cggtggatcg cgaggtgcgc acggtcgacg tcggggcgcg gccccgagcg
     9181 gaagaggccg gcatcgtcgg ccgcgccccc actcttcgcc ccgtcgaggc ctgcggcgag
     9241 cgccgcccgg tcgtgcgtcg gcgggaggat cggctcgccg cggaacagcc gcgcccagct
     9301 cgtcaggaag tggaagtagg agtagccgtc ggcgacggcg tgcgagaggc tgaagccgag
     9361 gacgcaaccg gagggcgtcc gcgtcaggag ggcccgcccg agcggctccc cttccttccc
     9421 gctcaccggg tcgaggaaga cgtccttgcg cggtgtcgcg tcccagtccg acggcgagga
     9481 ggcgaccgtg aaagcgcagc cgtccgcgca gggctcgaag gcgagagagc gctcgtcgag
     9541 ccggacgaag cggctcccga ccggcgggaa ggcctcgatc gcctcccgga ggctcgcctc
     9601 gaggcggccc gcgtcgatcg ctccctcgta ggcgaagacg aactcgatcg ggtaggcccc
     9661 gcggccggtg aagacgtggt cgatcggcga aagggggagg agcggcatgg cctggatgat
     9721 cgcccaggcc ggaatgaagc gggcacgccc ctcgccgggc gagcccctca gcgggcgtcg
     9781 aggacgcgca tctcggggag ctcgatcgcg gagaggaagc cccccattcc cgcccccgtg
     9841 tcgattcccg cgagcgacgc cgtgacgtag acgtcgagcg ggtccccggg ggtgtggacc
     9901 gactgatcct gggggagctt cctcaccggc gtgtgcccga agacgacccg cttccccttg
     9961 tagccttcgt agaaggaccg gctccgctgc cagaggagcg ggcgcgggtc cgcgacctcc
    10021 gacgggtgga gccagcgtcg gccgtcgtgc ggaagcccgg cgtggacgta gatcgcatgc
    10081 tcgtcctcgt ggaagtgcgg aagcccgccc atccacgcca cgacgtcggc cgggaggaac
    10141 gcgcccgtgg ccatcgcgcg aagctcgtcg gccgtcgggt tctcgccggg gcccggcacc
    10201 ggcccgcccg tgtacgaccg gtaggtcgaa tagcagccgt tcccgcgcgg caggacgaac
    10261 tcgggggcgc cctcgcggca ggccttcaac caggcgtcct cgtgattccc gcggaggaag
    10321 acgacgcggg ccgccgtctc ggacggcagc gacctcaccc gccggacgac gcccgcgggg
    10381 ttcgggccgc ggtcgacgta gtcgccgacg aagaggagcg tgtcttccgg gaggaggacg
    10441 ggcagcctcg cgaggagccg ttccagcccc tcgaggtccc cgtgcacgtc tccgatcgcg
    10501 atcgtccgcc ccgacatcgt ggtctcgttc cgcggacgat accggaggat gcccgggagc
    10561 agacgccttc gctgcgagcg agcgcggccg ccgagggccc cgcccggcgc ggagaggcct
    10621 ccggctgcta acctgacggg ccggaccgca cgggccagcg agccgcggtc ctccgggagg
    10681 attccatgcg cgctcggacc gggctccttc tcctcgccct tcccctcgcc ctcctcctcg
    10741 tccccgccgg atcgctcgcc gacgacgatc ccggcgtcgc cgcgcggctg aaggcgctcg
    10801 aggagcggat cgagagcctc gagttcgacc tcgaccaggt ccgcaagtcg agcgacgacg
    10861 ccctcttctg gctccgcctc ggagacgtcg ccgagatcga caaggtgaac gtcgccggcc
    10921 cgccgaaccc ccgtgcgaag gaccggtacg gcatcgcgaa cgagcggcac ccgatccgtt
    10981 tcgcgcagta cgtcttcgcg ccgaaggtcc gcgaggcggg gcggaagtac ccggtcgtcg
    11041 tccttccgca cggaggcgtg cacggcgact tcgggaccta ccacgtccac atcgtcaagg
    11101 agatgctcga gcaggggtgg gtcgtcctcg cccccgagta ccgcggctcg accggctacg
    11161 ggaagggctt ctacgaggcg atcgactacg gcggcctcga ggtggacgac gtgatcgccg
    11221 gcctcgactg ggccgtctcg gagctgccgt acgtcgacgg ctcccgtgcc gcgatcgccg
    11281 gctggagcca cggcggcctc atcgcgctcc tcgcggtgat gcaccacccg gagaagttcc
    11341 gcgcggccta cgccggcgtc ccggtgacgg acctcgtgac gcgcctcggc taccacgagc
    11401 agagctacga ggacctcttc gccgcgaagc accacgtcgg gaagaaggtc gtcgaggacg
    11461 tcgacgagta ccggcggcgg agcccggtct tctgggcttc gaagctgaag acgccgctcc
    11521 tcgtccacgc cacgacgaac gaccgcgacg tgaacgtgat cgaggccgag aacctgatcg
    11581 cggcgctcaa ggccgagggg aagagcttcg agcacaggat ctacacggac gctcccgccg
    11641 ggcactcctt caaccggatc gacacgcgcc tcgcgaagga atcccgcaag gagatctggg
    11701 cgttcctccg caagcacctc ggccgctgac ggctcggata gactgaaggg cgctgacccg
    11761 aacaggaggc gacgttggcc ggactcggca tctcggtccc ggggttcctc ctgcggctcc
    11821 tcgccgcggc gctcctcgtc ttcgccacct acaacccggc gggctggtcc ttcctccact
    11881 gggtgcggaa gagcggcggc gggcctctcc cgctgaaggt cctcgccggc atcgcgctcg
    11941 tcgccggctg ggtcttctgc gtccgggcga cgctccgctc gctcgggcac gtcgggacgg
    12001 cgctcgcggc ggcgttcttc ggcgtcctca tctggctctt cgtcgacctc cgggggaccg
    12061 acgtcgaggg ctcgacgctc gcctggatcg tcctcgcctg cctcgccgcg acgctcgccg
    12121 tcggcctctc cttctccctc gtgcggcggc gcgtcaccgg gcagatcgac gacacggacg
    12181 aggagtgagg cgggcatgag ccgctccgtc ctgcgagccg agatcggcga gcagcccgac
    12241 gtcctgaccc ggcttctcgt ccgggagggg ccccgcatcc gccggatcgc ccgcgacctc
    12301 gcccgtcgag acctccggtt cgtcctcgtc gccgcccgcg gcacctccga caacgccgcc
    12361 cgctacgctc agtacgcgct cgggctgacg tgccggctcc ccgtggcgct cgcggggccg
    12421 tcgctcgtga cgctctacga cagcccgccc cgcctgaagg gggcgctcgt cgtcggcatc
    12481 tcgcagtcgg ggcgctcgcc cgacgtcgtc gagacgatcg cctcggcgcg gcgcgaggga
    12541 gtgccgacgc ttgcgatcgt gaacgacccc gagagcccgc tggcgaaggc cgcggccgag
    12601 gtgatcccgc tccaggcggg cgtcgagcgg agcgtcgccg cgacgaagac gtacacggcc
    12661 cagctcctcg ccgtcgcgct cctcgccgcg ggcctgtcgg gctcgcggaa gcacgcggaa
    12721 gggctcgggg cccttcccga cgccgtggcg gacgcgctcg ggacggaagg ggccgcccgc
    12781 cgggccgccg cccgcctcgc gacggcggcc cgcctcgtcg tcctctcgcg cggcctgaac
    12841 taccccaccg cgcacgagct cgcgctgaag ctgaaggagc tggccctcct cgaggccgag
    12901 gccctctccg gcgccgactt ccggcacggc ccgatcgccc tggccgggcc gaagctcccg
    12961 gcgatcgtcg tctccccccc ggggaccgcg accgaggaag acctctccga cctcgccgcc
    13021 gagctccgcc gccgcgggag cccggtcctc gcgatcggcc cgcacggcag gggctccctc
    13081 ccggtcccgc ggctcccgga actcctctcc ccgatcgtcg cgatcgtgcc cggccagctc
    13141 ctcgcgctcc acgccgcgcg ggcgcggggc ctcgacccgg acgcgccccg cgggctctcg
    13201 aaagtcaccg agacccgctg accggaacgg cagtaatcta tcggatggtc cgcgcacagt
    13261 cgcggaaacg aatgctcgaa ggagtcgctc gatgaagaag accttcgccc tcgtcgcgtg
    13321 cgccctgctc gtggccggcc tcgccgcctg caacgccgac aagggccccg ccgacgccgc
    13381 catcaaggcc gccgatcagg cgatcacggc cgccgccccc gaggccgaga agttcatccc
    13441 ggagcagctc accgccgcca aggacgccct caaggccgcc caggaccagt tcgccaaggg
    13501 cgactacaag gccgccctcg ccgccgcgac cgagctcggt acgaaggccc aggagctcgc
    13561 caccgccgcc gcggccaaga aggacgagct gacgaaggcc tggacggacc tctccgggtc
    13621 gctcccgcag atggtcacgg cgatccaggc caaggtcgag gaactcggca aggcgaagaa
    13681 gctcccgaag gggatggaca aggccgccct gaccggggcc caggaagggc tcgccggcat
    13741 ccagaagtcg tgggaggagg cttcggccgc cttcaccggc ggcaacctcg tcgacgcaat
    13801 ggcgaaggcg gggggcctga aggagaaggc gacggagatc atgagcgccc tcggcctgca
    13861 ggctccggcc ccggccgagg ctccggctgc cccggcgaac tgattccgaa ttgacgtccc
    13921 gcgccgctcg cggcgcgcgt gcggccccgg cttcgccggg gccgctctct tttcctgggc
    13981 agcccccgcg cgagcgctcg gtcgaccggc cctctcagag cgcccggagg acgactcccg
    14041 ccgcccggag ccgctccgcg atcgcgcggg cgaaagcagg cgcacccggc gtgtccgagt
    14101 gaaggcagat cgtctgcgcg tcaagccgca cgcgcgcgcc gccggccgcg acgacctgcc
    14161 cgtcgagcac gatcgatagc gcctgccgag ccgcgtcctc aggagtctcc ttcatcgcac
    14221 ccgcccggct cctcgggatg agcgtgccgt cgggctcgta gcctcggtcc gcgaacgcct
    14281 ccccggcgac gcggaagccc gccccggcga aggcctcgag catctccgag ccggcgagac
    14341 cgacgaggac gacacccggc gcgacgcgcg cgacaccttc ggcgaaggcg cgggcgaggt
    14401 ccgggtcctt cgcggcggcg ttgtagagcg cgccgtgcgg cttgacgtgg gagaggcgcg
    14461 ccccgcagcg ggcggcgacc tcgaggagcg aggcgagctg ctcggcgacc gtcccggcga
    14521 ggagcccgcc cggcaggctc atcccgcgtc ggccgaagcc ggcgcggtcg gggtacgagg
    14581 ggtgcgcgcc gatcgcgacg ccgcgcgcga gcgccgcgcg gacgacgcgc tccatcgagg
    14641 cctcgtctcc cgcgtgcccg ccgcaggcga cgttgaccga ggtgacgact ccgaggagct
    14701 cctcctcgac ggtcgcgagg tccggaagct cgccgacgtc ggcgttcagg tcggtcgtcc
    14761 tcactcgagc tcccgccccc gcgtctccgg gaggagccgc atcaggagcg ccccggcgag
    14821 gtagagcgcc gaggtgaggg cgagggcgct cccgaggccg tgacggtccg cgagcgcgcc
    14881 gatcgagaag ggggcgagcg cgctcaccgc ccggccggcg ttgtagcaga agccctgcgc
    14941 ggccccgcgg acggacgacg ggaagagctc ggcgaggagg acgccgaaga cggagaagta
    15001 gccgtgcccg aggtagccga cggcgggcgc gaggaggagg agcgccgcct ccgaacgggc
    15061 gaggagcccg aacgcgggga ccacgacggc cgcgccgagg acgaacgcga ggaagacggg
    15121 acgccggccg aagcggtctg agaggaagcc gaaggtcgtg tagccgagga acgcccccgc
    15181 ctgcatcgcg acgacgaagc cggtcgagcg gacgaggccg aggcccgcgc ccccctgcgc
    15241 ggcgggcgcg gcgaggaacg tcgggaccca ggtgaagagt ccccagtagg cgaagaggag
    15301 gacggtggcg agggaggtcg ccgcgacggc gcggccgagg aggggcggct tcaggagcag
    15361 cgcgaggccc gttcctcccc cggcgagcgc cccgcgttcg cgccagatcg ccggctcggc
    15421 gacgcggcgg cggacccaga cggcgaggag gcccggcagg acgccgagga cgaagagcgg
    15481 ccgccacccc cacgccggga ggaccgccgc cgcgaggaac gcggcgagga cgtagccgac
    15541 ggcccaaccc gactgcatca ggccgatcgc cttgccgcgg tgggccgcgg gccacgtctc
    15601 ggcgacgagg accgagccgg cggaccactc cgctccgagg ccgaggccga cgagcgtccg
    15661 ccagagggcg agctcgagga cggtgcgcga cgtcgccgtg aggcccgtga agagggcgta
    15721 ggccaggatc gagagcacga ggacgcgggc ccgcccgaag cggtccgcga ggaggcccgc
    15781 tccgatcccg ccgacggccg acgcgacgag cgtcacggag gcgaggagcc ccgcccgcgc
    15841 gccgtcgagc ccgaactccg cgcggatcgc cgtcagggcg aaggcgtaga gcatgacgtc
    15901 catcgcgtcg agcatccagc ccgaggcggc cgcgaggagc gcgcggcggg cctcgggcga
    15961 ggcttcgcgg agccacgcga gcctcacgag aggaagaccc ccggcgcgtc gaggcgcgcc
    16021 tcgcggtcga ggaggagacg acgcgcctcc tcgaaggaga cccgggcgaa gcggacctcc
    16081 tcgcccgggc ggagctgggc gagggcggag aggtcggccg agatcacgct gccgatcttc
    16141 ggatatccgc ccgtcgaagg gtgctcgacg aaaaggacga tcgggaggcc cccgggtgga
    16201 atctggaccg cgccggcgac gaccccctcc gtgacgagcg agcgggccgg cgcggtgagc
    16261 gcctctcccc cgaggcggac ccccgtccgg ctcgaggagg ccgagacgcg gaacgaccgc
    16321 gagaagaacg cctcgacgac gtcctccccg aaccagtcgc cctgcggccc cggcgtcacg
    16381 cgcaagcggc ggcgggggcc gctccagcgc gccgcggcgg cgtcgacgcg ccgccctttc
    16441 ggcgctcccc gagccgggcc gacggagagt cggtcgcccg cgccgagcgg ccgaccgtcg
    16501 aggccgccga agccggtcgc cacgtccgtc gcacggctcc cgaggacggg cgggagatcg
    16561 agcccgcctc tcacgcagac gcaggcccgc gcccccgaat gggcgcgccc gaccgcgagc
    16621 cggccgccgg ccgggacctc caccgtcttc cacggcggga gcggcgcgcc gtcgaggcgc
    16681 gctccgaacg gcgctcccgc gaggacgacg agggcggcct cctcgaagag gagctcgggc
    16741 ccgacgagcg tcgcctcgag cgcggccgcc ccctcctcgt tcccgaggag gaggttcccg
    16801 aggcggaggg catgcgcgtc ggccgcgccg ccggccggaa cgccgaggcc ggcgagcccc
    16861 ggccggccga ggtcctgcac cgtcgtcagg aggccgggag agaggacccg gatcacggct
    16921 tctcccgacg gaggcggtcg aagtcccggc gcgggatcgg gacgaggcgg acggcgtctc
    16981 ccggggcgag gagcgcggcg ggctcgcgcg ccggctcgaa gagcgcaagc ggcgtccggc
    17041 cgacgaggcg ccacccgccc ggcgtcgggc gtgggtagag ccccgtctgc cctcccgcga
    17101 ttccgacgct cccggccggg acggcgaggc ggggcgccgg gaggcgcggc gtcgcgagga
    17161 ggggcggaag gccgaggagg taggcgaagc cgggggagaa accgaggaag gcgacgcggt
    17221 agggcgccgc cgcgtggagc tccgcggcct cttccggcga gagccccgcg ccgcgcgcga
    17281 cgtcggcgag gtcgggggcg cagtcgccct cgtagcagac cggaacctcc acgcaccgcg
    17341 cggcgggcgc gaaaggaagg ccgggcggcg ggaggagcgg ctcgacggcg cgcgagagct
    17401 ccgcgtgcga tgtcgcccgc gggtcgaagg cgacgaggag cgatgcgtag gcgggccgga
    17461 ggtcgaggag cccgcgcgga gggcgcgccg cgagcgccgc gcggagcgcg aggaccgcgg
    17521 ccgtcgccgc ctccgagggc ccttccccca ggacgacgag gagcgcggcg tcgctcgccg
    17581 gaagaaggcg cgggaggccg gccggggcgt ccttcgtcac gcgccccgac gatacgctcg
    17641 gaccgcccct cgacgcggcg cgcgcacggg attaccgtcg gagaaaacgg gcggaggctg
    17701 cgatggctct cgacgtcttt caccaggtcc tggcgaagct ccacgaggcg gccggcgggg
    17761 acccacggca gatggtcccg ttcgtcgagg tcctgaagca ggagaagctc ttcgggagcg
    17821 tcgacatgat cacgacccgc ctccagtcgg aggggtggat cgccgacgcg cccaagaagg
    17881 accacgtctt cgtcacgacc tggggcgtcg aggagctccg gcgtacgtcg gccgcgtccg
    17941 gcgccgcgaa gccggcagag ccgaagaacg ccgccgcgct ccgcgccgcg gcggagaggg
    18001 cgcgcgagct cgccgcgacg ctcgaggagc tggcctcggg gacggccggg accgccgcgc
    18061 agcggaaggc gaaggcgaag aaggccctcg ccgccgcgac ggaggcggtc gacgaggccc
    18121 tcggttgagc ctcgacgcgc tcctgccctc gcccgtccgc ttcctcgagg ggcggctcgg
    18181 gcgcctccgc gccccggagt ccctcgcccg gtacgaggcg tacttcgagg agcgcggcaa
    18241 ggcgatctcc gaggcggtcg accgtgccgg gacgccgaag ctgctcatgt tcgaccgctt
    18301 cgggacgcgg atcgacgagg tcgtcttccc gcccgcctac cacgagctcg tccggagggc
    18361 ttacgaggcg ggcgtcgtct tccgggccgt cgaggaaggc gacctcgcgg cctcgtacca
    18421 gatcggctac gtcacctcct tctacgacgc cgggctctac tgcccgcaca ccgtctcgct
    18481 ctccacggcg atcccgctcg cgaagtacgg ctcggccgag ctccgggcga agtacctcgg
    18541 aaagctcctg aagcgggacg cgaccgtctg gcagggggcg acctggatga ccgaggcaaa
    18601 gggcgggtcg gacctcggcg ccttcgtcga gaccgtcgcc cgccccgacg gcgagcgctg
    18661 gctcctgacc ggggagaagt acttcacgag caacgtgggc gccgagctcg cgatcgccgc
    18721 cgcgcgcccc gagggggctc ctcccggcgt ccgcggcctc gagctcttcc tcgtcccgcg
    18781 gcggcgtgac gacggcgccc tcaacttccg cgtccggcgc ctgaaggaga agatcggcac
    18841 ccgctccgtc ccgacgggcg aggtggagct cgacgggagc gaggcgtacc tcctcggcgc
    18901 gcggggccag ggggtctacc tcgtcctcga ggcgctgaac gtctcgcgcg tctgcaactc
    18961 gatcggcgcc gtcgcgctga cgcagagggc gctccacgag gcctggagct tcgccgaggg
    19021 gcgcgtcgcg ttcggcaggc cggtcgccga gcagccgctc ttcgcccgcc aggcccggga
    19081 gcgccacgag accctcctcg acgcgttcgc cctcgcctgg gaggccgtca cgctcctctc
    19141 ggaggtctgg cgcgagacgc cgcgctattc cgaccgctac cacctcttcc gcctcgtcgc
    19201 gcacctctcc aagtactgga ccgccgaggt cgcggtccag tcggcgaagt gggcgatgga
    19261 ggcgcacggc ggcgccggca tcctctccga ctacccggtc gagcggctct tccgcgaggc
    19321 gatcatcctc ggcatctggg aggggacgcc gcaccggcag gtcctcgacg g
//
LOCUS       DAOONL010000067        19209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2896_length_19209_cov_16.376527, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000067 DAOONL010000000
VERSION     DAOONL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19209
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_19209_cov_16.376527"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLP50_10865"
     CDS             <1..461
                     /locus_tag="PLP50_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPA52091.1"
                     /translation="VEGIGMPGHFLVRVGGAAGVHVDTFAGGQELSREGCRSLHDRIA
                     GTKSRWSDDFLRPNTIDQVLERVLNNLAATTREENDAAGSYRVATFLAALRPLSPERT
                     LQKAITAAAIGVTEEAASGFADVIERFPDSPEAERASQGLDSLAAGTTVN"
     gene            complement(475..1230)
                     /locus_tag="PLP50_10870"
     CDS             complement(475..1230)
                     /locus_tag="PLP50_10870"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPA52092.1"
                     /translation="MSDRRLAAGLVLLVLALLLLALAPSARSAPPDAAAILARADVPR
                     TALSEARLHLRVTTSGSGSATFTGEFAVLVKGPERIRIEFLAPADRGKLLLVNGKDAW
                     LVLPNTRNSIRVPRSHRVTGGFAVADVARTRFVDDYDATVERTDVLAGRTCDVLLLRA
                     KKGRDPAFPVLRVWVDRAESLTRKVIFLLPSGKTAREATFDEWGTLRGVRTVTRMTIV
                     DALRSGTTVVDYLDAERSKLDDALFDPPVPAGR"
     gene            complement(1223..2455)
                     /locus_tag="PLP50_10875"
     CDS             complement(1223..2455)
                     /locus_tag="PLP50_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPA52093.1"
                     /translation="MTLRWWRLAARNLLRHRRRTFLTGSIVVVGYVAATMTAGFVAQT
                     FGGLKDITIRGQGGHLRVVSAEAVGKTDEEAAGLLLDDAEELTRSVATSPDVRQAMPR
                     LSFFGLVAHGDRSVGYMGTGTVPALEKSATLAAEAVAEGTFLSDPEADEVMLGGGLAR
                     SLGVTVGGLVTVMTTTPDGGLNAVDAEVVAVLRWPIKELDDRVLFLPYAPAARLLKAE
                     GKATSIAVTLKEGTDLERAAGALRARLHRTGTPAAVKTWIESAPFYVAVKRLYAAIFL
                     FTGLVLATVVVLAAANTMTMSVFERTREIGTLLAIGMERGQVRRLFLAEGLLMGLAGS
                     VAGAVASLLIRAAVNAARIELPPPPGATSGGALHVELIPLAFGVGFVLMTVTLLVASW
                     WPARRAARLDPVEALSHV"
     gene            complement(2452..3156)
                     /locus_tag="PLP50_10880"
     CDS             complement(2452..3156)
                     /locus_tag="PLP50_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52094.1"
                     /translation="MTAPLLSATALTRTYAVGEERVVGVRDAALSLERGELAVLAGPS
                     GSGKTTLLNLLGLLDRPDSGRLELLGEDVSTLDDRGRARLRREKLGFVFQSHQLVPVL
                     SAEENVALALWIRGLPEAECRVRARAALAAVGLAGMEGRRPDAMSGGQRQRVAVARAI
                     VGEPALVLADEPTASLDSETAARLLDLFDALHAEKGIAFLFSSHDPRIVERVPRRVRL
                     VDGRIASDERTGGGAT"
     gene            complement(3153..3959)
                     /locus_tag="PLP50_10885"
     CDS             complement(3153..3959)
                     /locus_tag="PLP50_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPA52095.1"
                     /translation="MTEFLAFPFFQRALLAGFVVAVVCGALSFFVVLRRLAFVGSGVS
                     HAAFGGVALAALVGAPPGLGALLAALAVALATAKASGDGDLTEDTAVGVFTVAGMAIG
                     VVAISFLETNVDLFGLMFGNILTVDPRDLLALVAASALVVLLLVLYFRPLLLASIDED
                     GAFAAGVNVARMRLLVLVLLALTVVVALKVVGILLVSALLVLPGAVARALFSRWPGFL
                     LGSVSAALVMVLGGLLLSVALDVPSGAAIILTGTILFLGAVGAGRLRRNA"
     gene            complement(3956..4615)
                     /locus_tag="PLP50_10890"
     CDS             complement(3956..4615)
                     /locus_tag="PLP50_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA52096.1"
                     /translation="MSVAARFENVTVRFGGRPAVESTSFTIPAGMRTAMVGPNGGGKT
                     TLARVLLGILRPDDGRVAFLDEQGRELPRPRIGYLPQRSTLSPQAPVTGLDLVGLSLA
                     PGPGFGMSPEVEAEARRALGRAGLADDLATRPVGRLSGGQRQRVLLARAIAGTPAILL
                     LDEPDTALDAEGMKTLRRLVAEEVAAGRTIVYVTHDSDAMGGADAVFRIDGRLAQETR
                     A"
     gene            complement(4612..5703)
                     /locus_tag="PLP50_10895"
     CDS             complement(4612..5703)
                     /locus_tag="PLP50_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPA52097.1"
                     /translation="MSLSRRRLSLTLAIVAGAAGCAAPATRPAPSPQIAVATIAPLAD
                     LVQRVAGPDWTVRTVIPPGTSPHVFEPEPSDLRKLLGARAVFVAGAGYDDWMRKVLAA
                     CASRAPVHDAAASLGIGQGHAWTEGAGHDASAEERGAHDEDDHATHDHEVAGHEGHGH
                     EAGAHGPEPEGDPVGAGGAHDGHAHGILGEDPHWWLSPELAARCLAGIAEAFAGVDPI
                     HAEAYRARAREGAAELAELDREIVARLAPLRGARFVSAHRAWVYFADRYGLVEAASIE
                     PVPGREPSPRELKALVTEGRQGGLGRLFTEPQFPPAAARVVANEAGLDLTLVDPIGGV
                     PGRATYPELMRFNADAFARGLSGAAKERP"
     gene            5775..6086
                     /locus_tag="PLP50_10900"
     CDS             5775..6086
                     /locus_tag="PLP50_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52098.1"
                     /translation="MEHRDGHPHEHGPGCGHRAIRHGDHADYLHDGHLHSAGEAGVTE
                     HVLAVDRGRPAACAPVECPARHHPGCPHPRVPHGDHLDYLVDGALHHLHGDHCDLHGT
                     V"
     gene            complement(6093..6998)
                     /locus_tag="PLP50_10905"
     CDS             complement(6093..6998)
                     /locus_tag="PLP50_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52099.1"
                     /translation="MAVLDMNHGFPNLGHASVVGRLERIGAEERDRIGRSAPEVRVVS
                     YDVRRGLAIPSSASRFPIVVGTGGPGALDPRQNDGVSPLAQGIAEDPAWEAPLWRFFD
                     ALLARRGTALFGICHTFGLLARWAGFGEAVARGPEKGKSAGAVANRLTGEAEAHPWFG
                     GYYRTSREGRVRVLDSRLFDILPSGRGSGTILAHEASADGEGGEAVTMVELDRFADGS
                     PRIWAVNHHPEIGGAAEQRESLMRLASGREVGPGWLEERLRALDAFNVSQETERELRA
                     TRAFTFEEPVRAVLARALAERSGAT"
     gene            complement(7058..8059)
                     /locus_tag="PLP50_10910"
     CDS             complement(7058..8059)
                     /locus_tag="PLP50_10910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPA52100.1"
                     /translation="MKKIAIVGTGAWGTALAIHGARAGLAVSLLGYRDDIVDDLARTR
                     AHPALPGAEPLPSLVEPTMSPDEAFDGAEAVLWSVSVQFTASELERLGPWLRRGLPLA
                     STSKGIEIGTHRRTSQMMVDALGPDVPVAVLSGPTFAIEVARGLPTVAVVACPDAGVA
                     ERLQKGLASPTFRLYRSTDVVGVEIAGATKNVVAIAAGIVDGLKLGQNTRAALLTRAL
                     AEIRRLGTRLGGQPETFSGLAGVGDLILTATGDLSRNRRVGLALASGQSVEEALASLG
                     GQVAEGVPTAGAIVELARSVDVVMPISETVTEILAGTTKPREAVSRLMTRSLKEEGT"
     gene            complement(8056..9312)
                     /locus_tag="PLP50_10915"
     CDS             complement(8056..9312)
                     /locus_tag="PLP50_10915"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPA52101.1"
                     /translation="MTATASILAVGSELLGTTRLDTNSLFLTGELEAIGVRVVRKACV
                     VDGWEELLAELRFALERSPLLVVTGGLGPTEDDRTKEAVAHLLGRRLVRDEEILARLK
                     ERFRKRGYEMPAVNAKQADVIEGALVLSNRRGTAPGYLVEDGGRSIVLLPGVPHEMKA
                     LFVEAVVPRLTEGAPREGLHRRVLKVVGLGESLVEQLVKPVYEAHREQDVTILAAAPG
                     EVQLHFAARGTREEAGRVLDALEADFRAALGGSLFGRDEETLEGVVGRLLREAGRTLA
                     VAESCTGGMIAARLTDVAGASEVFPGGVVAYANEAKVALLGVSPESIARYGAVSEEVA
                     REMAEGARRRFGASAGLAVTGVAGPAGGTAEKPVGTVHVALDTESTTRHERLTLGGDR
                     AMIRRWTTSAALGMVRAFLNEGGGAK"
     gene            complement(9309..11252)
                     /locus_tag="PLP50_10920"
     CDS             complement(9309..11252)
                     /locus_tag="PLP50_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPA52102.1"
                     /translation="MLYRLERVHKSFGTKSVLVGATWQHDPGRVVGLVGRNGAGKSTL
                     LRIVQGHVEPDAGRVLLAGGTTFASLEQAVEPEGDEPLRDFVARAQEGLRGLEGEMRR
                     LEEAIAAASASGAGEAVEPLLAEHDALRERFERNGGYEADARVERVLLGVGLPREIWG
                     RPVGELSGGQKHRAQIARLLLGEATVLLLDEPTNHLDLAGLEFLETWLVERKAAAAQA
                     ALVVSHDRRFLNAVADRIVEVAHGQLEDYPGDYEAYRRLKAERDRVRAKAAEEQRKLV
                     ERTEDFIRRNIAGQKTKQAQARRKMLAKLERVEAPREDANEVTFRFEEARTSGDRVLE
                     LKGLVPGYAETGPLLSPVSFLLRRGERIALWGPNGCGKTTLLKTISRLLPPLAGSAVP
                     GTGVIVGYYDQEMRDLPSSWTVLDVVRELDPTLLDGEARDFLALFDFRGDEVEQRVET
                     LSGGEKGRLSLARIVFGGANLLLLDEPTNHLDLDSRERLEEALLDFEGTIVFVSHDRW
                     FVDRIATHVLEVKDRQASLWPGSWSDLVRRRAAEKAAAGDEAPSTGGTRPERAPVRRP
                     DRQAERQRTKARREVEALEEEVVRLEERIREIDAALADPATWKDGPRTRELTLARDDA
                     QRALEARYAAWEDAVKKLEEVPA"
     gene            complement(11319..12215)
                     /locus_tag="PLP50_10925"
     CDS             complement(11319..12215)
                     /locus_tag="PLP50_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPA52103.1"
                     /translation="MNELYQELLPLGREGVVPAGDELWQEGAKGDNVVLLVEGTFEVL
                     QSSPSGEPIVLRLARAGDVLGEMACLDGGARSAGLRAATDCRIRTIPAAEFRDFIRLR
                     PHLMEELFRIESQRIRSLSRMVSQSQHRAITDRLTSLYNYGFFIERLDLELERAVQMR
                     DAVSVVLFDIDHFKSYNDAHGHPAGNVVLVQVSDLLRRTARRGDIVARFGGEEFVVLL
                     YGASRREALQVAEAARARVEATAFAGGETQPLGRVTISGGVACFPSDARGATDLLKAA
                     DVRLYKAKGAGRNRVESGGDGP"
     gene            12304..14151
                     /locus_tag="PLP50_10930"
     CDS             12304..14151
                     /locus_tag="PLP50_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPA52104.1"
                     /translation="MADEDLLEKAFAEDEEAPRLASGNRVVPPQLLSKLRHDMRTPIH
                     QILGYAEMVEEDAIATGQTGCAADLGKIQDAARRLLEMVDTLQERLLPAVDEAALPEP
                     AHDELPATGLDPGPGPRVDVPPVTGPIRRADLDIPVAEPVEPAHLLVVDDNAENRDML
                     SRRLRAHGFTIAVAENGPEALRLLEEVPFDLVLLDVVMPGMSGLEVLRDLRGRHDAAD
                     LPVIMATARDDSNDVVTALRLGANDYVTKPLDFPVVLARVGTQLSLARAKARIGTLAR
                     DLEVRNRFIFETFGRYLSNEVVERLLQTPEGLKLGGETRKVTLLMSDLRGFTQVADRI
                     PPDRVVRLLNNYLGTMADLLLSFQGTIDEFIGDAILAIFGAPVQREDDALRAVACAVE
                     MQKAMERVNEFNRREGLPPVEMGIAIHTGEVVVGNIGSDRRAKYGVVGSPVNLTARIE
                     SYTVGGQILVSERTRREGGGQLSVGSEVVVQMKGFENPVSAWDVRGVAGGYDLFLPAR
                     DEPLHALRGPLAVRFATISGKRVDGPESEGLLVRLSMKEAEVETRERPDPFRNLRLRF
                     VGNDGRTIPGELYGKVSPPRAGTKGFGLRFTSVPPEIRSFLETVLAGSR"
     gene            complement(14158..14526)
                     /locus_tag="PLP50_10935"
     CDS             complement(14158..14526)
                     /locus_tag="PLP50_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA52105.1"
                     /translation="MAKILVVDDDEMNRDMLSRRLLRKGYEVALAVNGAEAIEKARAE
                     RPELILMDLSMPVLDGYEATRRLKADTSTSRIPIIGLSAHAMVGDREKALSAGCDDYD
                     TKPVELPRLLGKVESFLEAG"
     gene            14609..15607
                     /locus_tag="PLP50_10940"
     CDS             14609..15607
                     /locus_tag="PLP50_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA52106.1"
                     /translation="MAFSWLSGSDDVAELIAKRNYSRAAKVLRGQLAKDPANASLKQQ
                     LGDVLALDGKSYEAVELLWKLADDYATSGFVGKAIAVLKKVQRIDPTLTKVEEKLARL
                     VRQKDDESTLALRVRAGAMRRRTELETTPTPMPERPAARPPAAEPFVLDFEADDGAPP
                     EGASETAPRSLHPVGEEAAQGMVASPLFSDFSSEELVEVIKGLELLTYEAGDVIVAEG
                     APGDSLFVLTTGTVKAFVKDPDGHYHKVREMYEGAFFGEVSILTGKPRSATITAATPC
                     ELLALDRAALESIAERQPNVMTILRHFCEARYGSVDEQQIRRGDTHPGVSRRPSAV"
     gene            complement(15663..16973)
                     /locus_tag="PLP50_10945"
     CDS             complement(15663..16973)
                     /locus_tag="PLP50_10945"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPA52107.1"
                     /translation="MSLIVRTIVKKRDGGELSEGEIRDFVAGVTNRTVTDEQAAALLM
                     AICCRGMTTAETAALTFAMMNSGETWDLSPHGFVADKHSTGGVGDKSTLVLAPLLAAC
                     GVKTGMMSGRGLGHTGGTLDKLEAIPGFVTGQSKEAFDRLVEKVGCALIAATDTVAPA
                     DRRLYALRDVTGTVESIPLITASILSKKLATGAHAVVFDVKTGNGAFMQKPEDAHTLG
                     RALVDTTRATGRKASGYVTAMDRPVGIAVGNANEVEESIRLLRGDGPADLRENVLLLG
                     ADLLLAAGVADTEKEARRRLNASLQDGTALEAFARVIAEQGGNPAVCDDLALLPQPAA
                     KHEVKAAAAGTVRRVATRELGLLSIEIGCGRARKEDVVDPASGFRVFRKTGETVAAGE
                     PLLVVELGPRTQLPKGYLERVAACFEIGEGAGAEPALPFVVEKL"
     gene            complement(16970..18391)
                     /locus_tag="PLP50_10950"
     CDS             complement(16970..18391)
                     /locus_tag="PLP50_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA52108.1"
                     /translation="MRTAFAAALLLALPAAAAPAAKAPAKKAPCDLVVVAGHALPMDA
                     AFSVRAATAVAVRAGEIVAVGDPAAIDAAWAPKQRIERKDAVLLPGLVNTHGHAAMNL
                     LRGLSNDQPLMEWLTKYIFPAEGKNVSPEFVRAGTDLACLEMIRGGTTTFADMYYFES
                     DVAASVDRAGLRGVLGETWIDFPAPGHANLEETKAVTRAFLEKWKGHPRITPAIAPHA
                     PYTCSRETLQAARALADEYGAPLLTHVSETWDEQTQLREKHGKSPTAWLDEVGFLGPR
                     LCVAHGVWLSPEDLATLAARGVSLSHNPESNMKLGSGIAPVASARKAGVVVGLGTDGS
                     AGSNNDLDMFDAMDFAGKLAKVAALDPTPLAAPELLRMATIDGARALGLDRLVGSIET
                     GKRADLIVVDLAKAHVEPVYDLPSTVVYASRAGDVSLTVVDGKVLWDGKRVTTLDEAK
                     VLAAAREWRGKVLKSLETSAEKR"
     gene            complement(18403..18807)
                     /locus_tag="PLP50_10955"
     CDS             complement(18403..18807)
                     /locus_tag="PLP50_10955"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPA52109.1"
                     /translation="MTARSLGAAEREALVAAATAARENASAPYSHFHVGAALLAADGR
                     IFGGCNVESASYGLTCCAERTAVFKAISEGVRSFRAVAVVTGADEPTSPCGACRQVLW
                     DQCRDIAVVMATTGGKLEETTLASLLPRAFEL"
     gene            complement(18812..>19209)
                     /locus_tag="PLP50_10960"
     CDS             complement(18812..>19209)
                     /locus_tag="PLP50_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52110.1"
                     /translation="GVTGPSYETPAEIRAFRALGASAVGMSTVPEVIALSHMGVGVLG
                     VSCITNMAAGVLKQPLRHDEVLETTRRVKDRFVRLLTALVEAMGKGRSSDRPVVEPAR
                     RAKKAPRKVMTKLAAGLRRGAKPQARRRR"
BASE COUNT         2650 a         7165 c         6875 g         2519 t
ORIGIN      
        1 ccgtcgaggg gatcgggatg cccggccact tcctcgtccg cgtgggcggg gcggccggcg
       61 tccacgtcga cacgttcgcc gggggacagg agctctcgcg ggaaggctgc cgctcccttc
      121 acgaccggat cgccgggacg aagagccgct ggagcgacga cttcctccgg ccgaacacga
      181 tcgaccaggt cctcgagcgg gtcctgaaca acctcgcggc cacgacgcgc gaggagaacg
      241 acgcggccgg gagctacagg gtcgccacgt tcctcgcggc cctgcgcccg ctctccccgg
      301 agcggacgct gcagaaggcg atcaccgccg ccgcaatcgg cgtcacggag gaggccgcga
      361 gcggcttcgc cgacgtcatc gagcgcttcc ccgactcacc cgaagcggaa cgcgcctcgc
      421 aggggctgga ctcgctcgcc gccggtacga cggtgaactg accgcccggc cccgtcaccg
      481 cccggcggga accggcgggt cgaagagcgc gtcgtcgagc ttcgaccgct cggcgtcgag
      541 gtagtcgacg acggtcgtcc cggaccggag cgcgtcgacg atcgtcatcc gcgtcacggt
      601 ccggaccccg cggagcgtcc cccactcgtc gaacgtcgcc tctcgggccg tcttccccga
      661 cgggaggagg aagatcacct tccgcgtgag cgactcggcc cggtcgaccc agacgcgcag
      721 gaccggaaac gccgggtccc gccccttctt cgcccggagg agcaggacgt cgcaggtcct
      781 ccccgcgagg acgtccgtcc gctcgacagt ggcgtcgtag tcgtcgacga agcgggtccg
      841 cgcgacgtcc gcgacggcga agccgccggt cacccggtgg ctgcgcggca cgcggatcga
      901 gttgcgggtg ttcgggagga cgagccaggc gtccttcccg ttcacgagga ggagcttgcc
      961 gcggtccgcc ggcgcgagga actcgatccg gatcctctcg ggtcctttga cgaggacggc
     1021 gaactcgccg gtaaaggtcg cgctgccgga gcccgaggtc gtcacgcgga ggtgcaggcg
     1081 cgcctcggag agggccgtgc gcgggacgtc ggcgcgggcc aggatcgcgg ccgcgtccgg
     1141 cggggcgctc cgcgcggacg gcgcgagggc cagcaggagg agcgcgagga ccagcaggac
     1201 gaggccggcc gcgagtcggc ggtcagacat gggagagggc ctccacgggg tccagccggg
     1261 ccgcccgccg cgccggccac cacgacgcga cgaggagcgt gaccgtcatc aggacgaagc
     1321 cgacgccgaa cgcgagcggg atcagctcca cgtggagcgc gcccccgctc gtggcgcccg
     1381 ggggcggcgg gagctcgatc cgcgccgcgt tgaccgccgc gcgaatgagg agcgacgcga
     1441 cggccccggc cacggacccg gcgaggccca tcaggaggcc ctcggcgagg aagagcctcc
     1501 tcacctggcc gcgctccatc ccgatcgcca ggagcgtccc gatctcccgg gtcctctcga
     1561 agaccgacat cgtcatcgtg ttcgccgcgg cgaggacgac gaccgtcgcc aggacgagcc
     1621 ctgtgaagag gaagatcgcc gcgtagagcc tcttcacggc gacgtagaac ggggcgctct
     1681 cgatccaggt cttcacagcg gccggcgtgc cggtccggtg gagccgcgcc cggagcgccc
     1741 cggccgccct ctcgaggtcg gtcccctcct tcagcgtcac ggcgatcgag gtcgctttcc
     1801 cctcggcctt caggaggcgc gcggccggcg cgtacgggag gaagaggacc cggtcgtcga
     1861 gctccttgat cggccaccgg aggaccgcga cgacctcggc gtcgacggcg ttcaggccgc
     1921 catcgggtgt cgtggtcatg accgtcacga gaccgccgac cgtcacgccg agcgagcggg
     1981 cgaggccccc cccgagcatc acctcgtcgg cctccggatc ggagaggaac gtcccctcgg
     2041 cgaccgcctc cgcggcgagc gtcgcgctct tctcgagggc ggggacggtc ccggtgccca
     2101 tgtagccgac gctccggtcc ccgtgcgcga cgaggccgaa gaacgagagc cgcggcatcg
     2161 cctggcggac gtccgggctc gtcgcgacgc tccgggtcag ctcctcggcg tcgtcgagga
     2221 ggagaccggc cgcctcctcg tcggtcttcc cgacggcctc cgccgacacg acgcggaggt
     2281 gtccgccctg gccccggatc gtgatgtcct tcaacccgcc gaaggtctgg gcgacgaagc
     2341 cggcggtcat cgtcgcggcg acgtagccga cgacgacgat gctccccgtc aggaacgtcc
     2401 ggcgccggtg ccggaggagg ttgcgcgccg cgaggcgcca ccagcggagc gtcacgtcgc
     2461 gcctccgccg gtccgttcgt ccgaggcgat ccggccgtcg acgagacgga cgcgccgcgg
     2521 gacccgctcg acaatccgcg ggtcgtggct cgagaagagg aacgcgattc ccttctcggc
     2581 gtggagggcg tcgaagaggt cgaggaggcg cgcggccgtc tcggagtcga gagaggccgt
     2641 cggctcgtcc gcgaggacga gcgccggctc gccgacgatc gctcgcgcga cggcgacccg
     2701 ctgcctctgg ccgcccgaca tcgcgtcggg gcggcgtccc tccatcccgg cgagaccgac
     2761 ggcggcgagc gccgcacggg cgcggactcg gcactccgcc tccgggaggc cgcggatcca
     2821 gagggcgagg gcgacgttct cttcggccga caggaccggg acgagctgat gggactggaa
     2881 gacgaagccg agcttctcgc gccggagcct cgcgcgcccg cggtcgtcga gcgtcgagac
     2941 gtcctcgccg aggagctcga ggcggccgga gtcgggccgg tcgaggaggc cgaggaggtt
     3001 caggagcgtc gtcttgccgc tccccgacgg gccggcgagg accgcgagct cgccccgctc
     3061 gagggagagc gcggcgtccc tcacgccgac gacccgctcc tcgcccaccg cgtaggtccg
     3121 ggtcagggcg gtcgcggaga ggagcggcgc cgtcacgcgt tccgccggag ccgccccgcg
     3181 ccgaccgcgc cgaggaacag gatcgtgccg gtgaggatga tcgccgcgcc cgaagggacg
     3241 tcgagcgcga cggagaggag gagcccgccg aggaccatga cgagcgccgc cgacaccgac
     3301 ccgaggagga agccgggcca gcgcgagaac agtgcccgcg cgaccgcgcc gggaaggacg
     3361 agcagggccg agacgaggag gatgccgacg accttcagcg cgacgacgac ggtcagcgcg
     3421 aggaggacga ggacgaggag ccgcatccgc gccacgttca cgccggccgc gaacgctccg
     3481 tcctcgtcga tggaggcgag gaggagcggc cggaagtaga ggacgaggag gaggacgacg
     3541 agagccgagg ccgccaccag ggcgaggagg tcccgcgggt cgaccgtcag gatgttcccg
     3601 aacatgaggc cgaagaggtc gacgttcgtc tcgaggaagc tgatggcgac gacgccgatc
     3661 gccatccccg cgacggtgaa gacgcccacc gcggtgtcct cggtcaggtc gccgtcgccg
     3721 gacgccttgg ccgtcgcgag ggccaccgcc agggcggcca ggagcgcccc gagcccgggc
     3781 ggggcgccga cgagcgcggc gagggccacg ccgccgaagg ccgcgtgcga gacgccggag
     3841 ccgacgaacg cgagcctccg gaggacgacg aagaacgaga gcgccccgca gacgaccgcc
     3901 acgacgaagc cggcgaggag cgcccgctgg aagaacggga acgcgaggaa ctccgtcacg
     3961 cgcgcgtctc ctgggcgagg cgcccgtcga tccggaagac ggcgtcggcg ccccccatcg
     4021 cgtcgctgtc gtgcgtgacg tagacgatcg tccgccccgc ggcgacctcc tcggcgacga
     4081 gccggcggag cgtcttcatt ccttccgcgt cgagcgccgt gtcgggctcg tcgaggagga
     4141 ggatcgccgg cgtccccgcg atcgcgcggg cgaggaggac acgctgcctc tggccgcccg
     4201 agagccggcc gacggggcgc gtggcgaggt cgtcggcgag gccggccctc ccgagcgccc
     4261 gccgcgcttc cgcctcgacc tcgggcgaca tcccgaagcc ggggccgggc gcgagggaga
     4321 gtccgacgag gtcgagaccg gtcacgggcg cctgcggcga gagcgtcgac cgctgcggga
     4381 ggtagccgat tcgcgggcgc ggcagctccc gcccctgctc gtcgaggaac gcgacgcggc
     4441 cgtcgtccgg ccggaggatc ccgaggagga cccgcgcgag cgtcgtcttt ccgccgccgt
     4501 tcggtccgac catcgccgtg cgcatcccgg ccgggatcgt gaacgaggtg gactcgaccg
     4561 ccggccggcc gccgaagcgg accgtcacgt tctcgaagcg ggcggcgacg ctcacggcct
     4621 ctccttcgcc gcgccggaga gtccccgtgc gaaggcgtcg gcgttgaagc gcatcagctc
     4681 aggatacgtc gcccgaccgg ggacgccccc gatcgggtcg accagcgtca ggtcgaggcc
     4741 cgcctcgttc gcgacgaccc gcgccgctgc cggcgggaac tgcggctcgg tgaagagacg
     4801 gcccagcccc ccctgccggc cttccgtcac gagcgccttc agctcgcgcg gcgagggctc
     4861 gcgtcccggc acgggctcga tcgaagcggc ctccacgaga ccgtaccgat cggcgaagta
     4921 gacccaggcc cggtgcgccg agacgaaccg cgccccgcgg agcggcgcga gccgggccac
     4981 gatctcccga tcgagctcgg cgagctccgc ggcgccctcc cgggcccggg cccggtaggc
     5041 ctcggcatgg atcgggtcga cgccggcgaa cgcctccgcg attccggcga ggcagcgggc
     5101 cgccagctcg ggcgagagcc accagtgcgg atcctcgccg aggatgccgt gggcgtggcc
     5161 gtcgtgagcc cctcccgctc ccaccgggtc gccttccggc tcggggccgt gcgccccggc
     5221 ctcgtgtccg tggccctcgt gcccggcgac ctcgtggtcg tgcgtcgcgt gatcgtcctc
     5281 gtcgtgcgcg ccgcgctcct ccgccgacgc gtcgtgaccg gcgccctccg tccacgcgtg
     5341 tccctgtccg atcccgaggg aggccgccgc gtcgtggacc ggcgcccgcg acgcgcaggc
     5401 cgcgaggact tttcgcatcc agtcgtcgta acccgctccc gccacgaaga ccgcccgagc
     5461 cccgaggagc ttccgcaggt cggacggctc cggctcgaac acgtgcggcg acgtccccgg
     5521 cgggatcacg gtccgcacgg tccagtcggg cccggcgacg cgctggacga ggtcggcgag
     5581 aggggcgatc gtcgcgaccg cgatctgggg ggaaggcgcg ggacgcgtcg cgggcgccgc
     5641 gcagcccgcc gcgcccgcga cgatcgccag cgtcagggaa aggcgcctcc gggacaggct
     5701 catcggcgtc ccgccccggc ttgacctcgg cccgctcccg ggccgatgct ccccatccag
     5761 gagggcgaca cgccgtggag cacagggacg ggcatcccca cgagcacgga ccgggctgcg
     5821 ggcatcgggc gattcggcat ggcgatcacg ccgattatct ccacgacggg cacctccatt
     5881 ccgccggcga agcgggcgtg accgagcacg tcctcgccgt cgatcgcgga cgcccggccg
     5941 cctgcgcccc cgtcgagtgc cctgcccggc accacccggg atgcccgcac ccgcgggtcc
     6001 ctcacggcga ccacctcgac tacctcgtcg acggcgccct ccatcacctt cacggcgacc
     6061 actgcgacct ccacggcacc gtctgagccc gctcaggtcg ccccggagcg ctccgcgagc
     6121 gcccgcgcga gaacggcccg gacgggctcc tcgaacgtga acgccctcgt cgcacgaagc
     6181 tccctttccg tctcctgcga gacgttgaag gcgtccagcg cccgaagccg ctcctcgagc
     6241 cacccgggcc cgacctcccg cccggaggcg aggcgcatca ggctctccct ctgctccgcc
     6301 gcgccaccga tctcggggtg atggttcacc gcccagatcc tcggcgaccc gtccgcgaaa
     6361 cggtccagct cgaccatcgt caccgcctcg ccgccttccc cgtccgcact ggcctcgtgg
     6421 gcgaggatcg tgcccgagcc gcggcccgac gggaggatgt cgaagagccg gctgtcgagg
     6481 acgcggaccc gcccttcgcg gctggtccgg tagtagccgc cgaaccaggg gtgcgcctcc
     6541 gcctctcccg tgaggcggtt cgcgacggcc cccgcgctct tccccttctc cggcccgcgg
     6601 gccacggcct ctccgaagcc cgcccatcgg gccagcaggc cgaaggtgtg gcagatgccg
     6661 aagagcgcgg tgccgcgacg cgccaggagg gcgtcgaaga agcgccagag gggcgcctcc
     6721 cacgccggat cctcggcgat cccctgggcg aggggcgaga cgccgtcgtt ctggcggggg
     6781 tcgagggcgc ccggtccgcc cgtaccgacg acgatcggga agcgggacgc gctcgacggg
     6841 atcgcgagcc cgcgccggac gtcgtacgag acgacgcgga cctcgggggc ggatcgtccg
     6901 atccgatcgc gctcttccgc gccgatccgc tcgaggcgcc cgacgaccga ggcgtgcccg
     6961 agattcggga agccgtggtt catgtcgagg accgccacgt cgaggaacgc gcgctccgcc
     7021 ggcggctctt cgccggggcg gtcgacccgg gcgtacgtca ggtcccttcc tccttgagcg
     7081 agcgcgtcat gaggcgggag accgcttcgc gtggtttcgt cgtcccggcg aggatctccg
     7141 tgaccgtctc ggagatcggc atgacgacgt cgacgctccg cgccagctcg acgatcgcgc
     7201 cggccgtcgg gaccccctcc gcgacctggc cgccgagcga ggccagcgcc tcttcgacgc
     7261 tctggcccga cgcgagagcg aggccgaccc tccggttccg cgagaggtcg cccgtcgccg
     7321 tcaggatgag gtcgccgacg ccggcgagac cggagaaggt ctccggctgg ccgccgaggc
     7381 gcgtccccag cctccggatc tccgcgagcg cccgtgtcag gagcgccgcc cgggtgttct
     7441 gtccgagctt caggccgtcg acgatccccg cggcgatcgc gacgacgttc ttcgtggcgc
     7501 cggcgatctc gacgccgacg acgtcggtcg agcggtagag acggaaggtc ggcgaggcga
     7561 ggcccttctg gagccgctcg gcgacgcccg cgtccgggca ggcgacgact gcgaccgtcg
     7621 ggaggccgcg cgccacctcg atggcgaacg tcgggccgga gaggacggcg accgggacgt
     7681 ccggcccgag ggcgtcgacc atcatctggc tcgtccggcg gtgggtcccg atctcgatcc
     7741 ccttcgaggt cgaggcgaga ggcaggccgc ggcgcagcca cgggcccagc cgctccagct
     7801 cgctcgcggt gaactggacc gagaccgacc agaggaccgc ctcggcgccg tcgaaagcct
     7861 cgtccgggct catcgtcggc tcgacgaggg acgggagcgg ctcggcgccg ggcagcgcgg
     7921 ggtgggcgcg cgtccgggcc aggtcgtcga cgatgtcgtc gcggtagccg aggaggctga
     7981 cggccagccc ggcccgtgcg ccgtggatgg cgagagccgt tccccatgcg cccgtcccga
     8041 cgatcgcgat cttcttcact tcgccccccc tccttcgttc agaaaggccc ggaccatccc
     8101 gagcgccgcc gaggtcgtcc agcggcggat catcgcccga tcgccgccga gggtcagccg
     8161 ctcgtggcgg gtcgtcgact ccgtgtcgag cgcgacgtgc accgttccga ccggcttctc
     8221 cgcggtcccg cccgccggtc cggcgacgcc ggtgaccgcg agccccgcgg aggcgccgaa
     8281 gcggcgtctc gctccctccg ccatctcccg ggccacctcc tccgagacgg cgccgtaccg
     8341 cgcgatcgac tccggcgaaa cgccgaggag cgccaccttc gcctcgttcg cgtacgcgac
     8401 gacgcccccc ggaaagacct cggaagcccc ggcgacgtcc gtgagccggg ccgcgatcat
     8461 cccgcccgtg cacgactcgg cgacggcgag cgtccgtccg gcctcgcgca ggagccgccc
     8521 gacgaccccc tcgagggtct cctcgtctcg gccgaagagc gatcccccga gcgccgcgcg
     8581 gaagtcggcc tcgagcgcgt cgagcacccg cccggcctcc tctcgcgtcc cccgcgcggc
     8641 gaagtggagc tggacctctc ccggcgctgc ggcgaggatg gtcacgtcct gctcccgatg
     8701 ggcctcgtag acgggcttca cgagctgctc gacgaggctc tcgccgagcc cgacgacctt
     8761 caggacccgc cggtggagcc cctcccgggg agctccttcc gtgagccgcg ggacgacggc
     8821 ctcgacgaag agcgccttca tctcgtgcgg gacgcccggc aggaggacga tcgaccgtcc
     8881 cccgtcctcg acgaggtagc cgggcgccgt cccccgccgg ttcgagagga cgagcgcccc
     8941 ctcgatcacg tcggcctgct tcgcgttcac cgcgggcatc tcgtatcccc gcttccggaa
     9001 acgctccttc agcctcgcga ggatctcctc gtcccggacg agccggcgac cgaggaggtg
     9061 cgccacggcc tccttcgtcc ggtcgtcctc ggtcggcccg agaccgcccg tcacgacgag
     9121 gaggggcgac ctctcgagcg cgaaccgcag ctcggcgagg agctcctccc agccgtcgac
     9181 gacgcacgcc ttgcggacga cgcgcacccc gatcgcctcg agctcccccg tcaggaagag
     9241 cgagttcgtg tcgagccgcg tcgtgccgag aagctcgctc ccgaccgcca ggatcgaggc
     9301 cgtcgccgtc acgccggcac ctcctcgagc ttcttcaccg cgtcctccca cgccgcgtag
     9361 cgcgcctcga gcgcccgctg cgcgtcgtct cgggcgagcg tcagctcgcg ggtccgcggc
     9421 ccgtccttcc acgtcgccgg gtcggcgagc gccgcgtcga tctcccggat cctctcctcc
     9481 agccgcacga cctcctcctc gagcgcctcg acctcgcgcc gggccttcgt ccgctgccgc
     9541 tcggcctggc ggtcgggccg ccggaccggg gccctctcgg gcctcgtccc gcccgtggaa
     9601 ggcgcctcgt ccccggcggc ggccttctcg gccgcccgcc gccggacgag gtccgaccag
     9661 ctccccggcc agagcgacgc ctgccggtcc ttcacctcga ggacgtgggt cgcgatccgg
     9721 tcgacgaacc agcggtcgtg cgagacgaag acgatcgtcc cctcgaagtc gagaagggcc
     9781 tcctcgagcc gctcgcgcga atcgaggtcg aggtggttcg tcggctcgtc gaggaggagg
     9841 aggttcgcgc cgccgaagac gatccgggcg agcgagagcc gccccttctc gccgccggag
     9901 agcgtctcga cccgctgctc cacctcgtcg ccccggaagt cgaagagagc gaggaaatcg
     9961 cgcgcctcgc cgtcgaggag cgtcgggtcg agctcgcgga cgacgtcgag gaccgtccac
    10021 gaggacggga ggtcgcgcat ctcctggtcg tagtagccga cgatcacccc ggtccccggg
    10081 acggcgctcc ccgcgagggg cgggaggagg cgcgatatcg tcttcaggag cgtcgtcttc
    10141 ccgcagccgt tcggccccca gagagcgatc cgctcgcccc gacgcaggag gaacgagacg
    10201 ggcgaaagga gcggaccggt ctccgcgtac ccggggacga gccccttcag ctccagcacg
    10261 cggtcgccgg aggtgcgcgc ctcctcgaac cggaacgtca cctcgttcgc gtcctcccgc
    10321 ggcgcctcca cgcgctcgag cttcgcgagc atcttccggc gcgcctgggc ctgcttcgtc
    10381 ttctgccccg cgatgttccg ccggatgaag tcctcggtcc gctcgacgag cttccgctgc
    10441 tcctcggccg ccttcgcccg gacccggtcc cgctccgctt tcaggcgccg gtacgcctcg
    10501 tagtcgcccg ggtagtcctc gagctgcccg tgggcgacct ccacgatccg gtcggcgacg
    10561 gcgttcagga accgccggtc gtgagagacg acgagggccg cctgagcggc ggcggccttc
    10621 cgctccacga gccacgtctc gaggaactcc aggccggcca ggtcgaggtg gttcgtcggc
    10681 tcgtcgagca ggaggacggt ggcctcgccg aggaggaggc gcgcgatctg cgcccggtgc
    10741 ttctggccgc cggagagctc cccgaccggc cggccccaga tctcccgcgg gaggccgacg
    10801 ccgagcagga cgcgctcgac gcgggcgtcg gcttcgtatc cgccgtttcg ctcgaagcgc
    10861 tcgcgcaggg cgtcgtgctc ggccaggagc ggctcgaccg cctcgccggc cccggaggcg
    10921 gacgccgccg cgatcgcctc ttccagccgg cgcatctccc cttcgagccc gcgcagcccc
    10981 tcctgcgccc gcgcgacgaa gtcccggagc ggctcgtccc cctccggctc gacggcctgc
    11041 tcgagggacg cgaatgtcgt cccgcccgcg agaagcaccc gccccgcatc gggctcgacg
    11101 tggccctgga cgatccggag gagggtcgac ttcccggcgc cgttccgtcc gacgaggccg
    11161 acgacccgcc ccgggtcgtg ctgccaggtc gctccgacga ggacgctctt cgtgccgaaa
    11221 gacttgtgga cgcgctcgag ccgatagagc acggccggag gataacctcc gagtcccgag
    11281 gccgctgcgg gagggtccgg gccgaggccg ccccgcggct acggcccgtc gccgcccgat
    11341 tcgacgcgat tccggccggc gcccttcgcc ttgtagagcc ggacgtcggc cgccttcagg
    11401 aggtcggtcg cgccgcgtgc gtccgagggg aagcacgcga cccctccgct gatcgtcacg
    11461 cggcccaggg gctgggtctc ccccccggcg aacgccgtcg cctcgacccg cgcgcgggcg
    11521 gcctcggcca cctggagcgc ctcgcgccgc gaggcgccgt aaaggaggac gacgaactcc
    11581 tctcctccga accgggcgac gatgtcgccg cgccgcgcgg tccggcgaag gaggtcggag
    11641 acctgcacga ggacgacgtt ccccgcgggg tggccgtgcg cgtcgttgta ggacttgaag
    11701 tggtcgatgt cgaagaggac gacgctgacg gcgtcgcgca tctggaccgc gcgctcgagc
    11761 tcgaggtcga gccgctcgat gaagaagccg tagttgtaga gcgaggtgag cctgtcggtg
    11821 atggcgcggt gctggctctg cgagaccatc cgcgagaggc tgcggatcct ctgcgactcg
    11881 atccggaaga gctcctccat caggtgcggc cggaggcgga tgaagtcccg gaactcggcc
    11941 gccgggatcg tccggatgcg acagtccgtg gcggcccgga ggccggccga ccgggccccg
    12001 ccgtcgaggc aggccatctc tccgaggacg tcgccggcgc gcgcgaggcg caggacgatc
    12061 ggctcgccgg acggcgagga ctggaggacc tcgaacgtcc cttccacgag gaggacgacg
    12121 ttgtcgccct tcgccccctc ctgccagagc tcgtcgccgg cggggacgac gccctcccgt
    12181 ccgagcggca gcagctcctg gtaaagctcg ttcacggcac ttccggctcc tcgcgttccg
    12241 atgcccgaag tatatcgaac cgctcgcggg tcacccgtcg agcggcgata cactcgtcgc
    12301 gatatggcgg acgaggacct gctcgaaaag gcgttcgcgg aggacgagga ggcgcctcgc
    12361 ctcgcgtcgg ggaaccgtgt cgtgccgccg cagctcctct cgaagctccg gcacgacatg
    12421 cggactccga tccaccagat cctcggctac gcggagatgg tcgaggagga cgccatcgcc
    12481 acgggccaga ccgggtgcgc cgccgacctg gggaagatcc aggacgccgc ccgccgcctt
    12541 ctggagatgg tcgacaccct ccaggaaagg ctgctccccg cggtcgacga ggccgcgctg
    12601 ccggagcccg cgcacgacga gctgccggcg accggtctcg atcccggccc gggcccgcgc
    12661 gtcgacgtcc cacccgtgac cgggccgatt cgccgcgccg acctcgatat cccggtcgcc
    12721 gagcccgtcg agccggcgca tctcctcgtc gtcgacgaca acgcggagaa ccgcgacatg
    12781 ctctcccgcc gcctccgggc gcacggcttc acgatcgcgg tcgcggagaa cggcccggag
    12841 gcgcttcgcc tcctcgagga ggtcccgttc gacctcgtcc ttctggacgt cgtcatgccc
    12901 gggatgagcg gcctcgaggt gcttcgggac cttcggggcc gccacgacgc cgcggacctc
    12961 cccgtgatca tggccaccgc ccgggacgac tcgaacgacg tcgtgaccgc gctccgcctc
    13021 ggcgccaacg actacgtcac gaagccgctc gacttcccgg tcgtcctcgc ccgggtcggc
    13081 acgcagctct ccctcgcgcg ggcgaaggcc cggatcggga cgctcgcgcg ggacctcgag
    13141 gtgcggaacc ggttcatctt cgagacgttc ggccgctacc tctcgaacga ggtcgtcgag
    13201 cggctcctcc agacgcccga gggcctcaag ctgggaggcg agacgcggaa ggtcacgctc
    13261 ctgatgtcgg acctccgcgg ctttacgcag gtcgccgacc ggatcccccc ggaccgcgtc
    13321 gtcaggctcc tgaacaacta cctcgggacg atggccgacc tcctcctctc gttccagggg
    13381 acgatcgacg agttcatcgg ggacgcgatc ctggcgatct tcggcgcccc cgtccagcgg
    13441 gaggacgacg ccctgcgcgc cgtggcctgc gccgtcgaga tgcagaaggc gatggaacgc
    13501 gtgaacgagt tcaaccggcg cgaggggctc ccgccggtcg agatggggat cgcgatccac
    13561 accggcgagg tcgtcgtcgg gaacatcggc tccgaccgcc gggccaagta cggcgtcgtc
    13621 gggagccccg tgaacctgac cgcgcgcatc gagtcgtaca cggtcggcgg ccagatcctc
    13681 gtcagcgagc ggacccggcg cgaaggcggg ggccagctct cggtcgggag cgaggtcgtc
    13741 gtccagatga agggcttcga gaaccccgtc tccgcctggg acgtgcgggg ggtggccgga
    13801 ggatacgacc tctttctccc ggcccgcgac gagcccctgc acgcgcttcg gggtcctctc
    13861 gccgtccggt tcgccacgat ctccggcaag cgggtggacg gcccggaatc cgaagggctc
    13921 ctcgtgcggc tgtcgatgaa ggaggcggag gtcgagacgc gcgagcgtcc cgacccgttc
    13981 cgcaacctgc gcctccggtt cgtcggcaac gacggccgga cgattccggg ggagctctac
    14041 ggcaaggtct ccccgccgcg ggccgggacg aagggtttcg gcctccggtt cacgtcggtc
    14101 cctccggaga tccgctcctt cctcgagacg gtcctcgcag gcagccgctg agggcctcta
    14161 ccccgcttcg aggaacgact cgaccttccc gaggagccgc ggcagctcga ccggcttcgt
    14221 gtcgtagtcg tcgcagcccg ccgagagcgc cttctcgcgg tctccgacca tcgcgtgcgc
    14281 cgaaaggccg atgatcggga tccgcgacgt ggacgtgtcc gccttcagcc gccgggtcgc
    14341 ctcgtagccg tccaggaccg gcatcgacag gtccatcagg atcagctcgg gcctctcggc
    14401 gcgagccttc tcgatcgcct ccgctccgtt cacggcgagt gccacctcgt accccttccg
    14461 gaggaggcgt cgcgagagca tgtcgcggtt catctcgtcg tcgtcgacga ccaggatctt
    14521 cgccatcggt gccgcccgga gtgtagcgcc ccgccgcctt gacagcagcc ggaacgctgc
    14581 cctaccgtcg agccggagta gatgtcgaat ggcgttttcg tggctctccg gctctgacga
    14641 cgtcgccgag ttgatcgcga agcgcaacta cagccgcgcg gcgaaagtcc tccgcggcca
    14701 gctcgcgaag gaccccgcga acgcttccct gaaacagcag ctcggggacg tcctggccct
    14761 cgacggaaag agctacgagg ccgtcgagct cctctggaag ctcgccgacg actacgcgac
    14821 ctccgggttc gtcggcaagg cgatcgccgt gctgaagaag gtccagcgga tcgacccgac
    14881 cctcacgaag gtcgaggaga agctcgcgcg cctcgtgcgt cagaaggacg acgagtcgac
    14941 cctcgcgctc cgcgtccgcg cgggcgccat gcgtcggcgg accgagctgg agaccacgcc
    15001 gacacccatg ccggagcggc cggccgccag gccgccggcc gccgagccgt tcgtcctcga
    15061 cttcgaggcc gacgacggcg cacccccgga gggcgcctcc gagacggcgc cccgcagcct
    15121 ccaccccgtc ggcgaggagg cggcgcaggg gatggtcgcg agccctctct tcagcgactt
    15181 ctcgagcgag gagctcgtgg aggtcatcaa ggggctcgag ctcctcacct acgaggcggg
    15241 cgacgtcatc gtcgccgagg gggcgccggg cgactccctc ttcgtcctga cgaccggaac
    15301 ggtcaaggcc ttcgtgaagg acccggacgg gcactaccac aaggtccgcg agatgtacga
    15361 aggcgccttc ttcggcgagg tctccatcct caccgggaag ccccggagcg cgacgatcac
    15421 cgcggcgacg ccgtgcgagc tgctcgccct cgaccgggcg gccctggagt cgatcgcgga
    15481 gcgccagccg aacgtcatga cgatcctccg gcacttctgc gaggcccggt acgggagcgt
    15541 cgacgagcag cagatccggc gaggcgacac ccatcccggc gtgagccgcc gcccgtcggc
    15601 cgtctgaggg cgcgaagggc cggcgtctcc gcgcgagtca gagacgccgg acccgcccgg
    15661 gctcagagct tctcgacgac gaacgggagc gccggctcgg cgccggctcc ctcgccgatc
    15721 tcgaagcagg ccgcgacgcg ctcgaggtag cccttcggga gctgcgtccg ggggccgagc
    15781 tcgacgacga ggagcggctc ccccgccgcg accgtctccc cggtcttccg gaagacgcgg
    15841 aaacccgagg cggggtcgac gacgtcctcc ttccgggcgc gcccgcagcc gatctcgatc
    15901 gagaggaggc cgagctcgcg cgtcgcgacg cgccggaccg tcccggcggc cgcggccttc
    15961 acctcgtgct tcgcggcggg ctgcgggagg agcgcgaggt cgtcgcagac ggccgggttc
    16021 ccgccctgct cggcgatcac gcgggcgaac gcctcgaggg ccgtcccgtc ctggagcgag
    16081 gcgttcaggc gccggcgcgc ctccttctcc gtgtcggcga cgcccgcggc gagcaggagg
    16141 tcggcgccga ggaggaggac gttctcgcgg aggtcggccg ggccgtcgcc ccggaggagg
    16201 cggatcgact cctcgacctc gttcgcgttc ccgacggcga tgccgacggg gcggtccatc
    16261 gccgtgacgt agccgctcgc cttccgcccc gtggcccggg tcgtgtcgac gagggcgcgg
    16321 ccgagcgtgt gcgcgtcctc cggcttctgc atgaaggcgc cgttccccgt cttcacgtcg
    16381 aagacgacgg cgtgcgcgcc cgtggcgagc ttcttcgaga ggatcgaggc cgtgatgagc
    16441 gggatcgact cgaccgtccc cgtcacgtcg cggagcgcgt agagccgccg gtcggccggg
    16501 gcgacggtgt ccgtcgcggc gatgagggcg cagccgacct tctcgacgag ccggtcgaac
    16561 gcctccttcg actgcccggt gacgaagccg ggaatcgcct cgagcttgtc gagcgtgccg
    16621 cccgtgtggc ccaggccgcg ccccgacatc atcccggtct tcacgccgca ggcggcgagg
    16681 agcggcgcga ggacgagggt cgacttgtcg ccgacgcccc cggtcgagtg cttgtcggcg
    16741 acgaagccgt ggggcgacag gtcccaggtc tcgcccgagt tcatcatggc gaacgtgagg
    16801 gccgccgtct ccgcggtcgt catcccgcgg cagcagatcg ccatcaggag ggccgccgcc
    16861 tgctcgtcgg tcacggtgcg gttcgtgacc cccgcgacga agtcccggat ctccccttcc
    16921 gacagctcgc cgccgtcgcg cttcttgacg attgtccgaa cgatgaggct catctcttct
    16981 ccgcgctcgt ctcgagcgac ttcaggacct tcccccgcca ctcccgcgcc gcggcgagga
    17041 ccttcgcctc gtcgagcgtc gtgacccgct tcccgtccca gaggaccttc ccgtcgacga
    17101 cggtgagcga gacgtcgccg gcgcgcgagg cgtagacgac ggtggagggg aggtcgtaca
    17161 cgggctcaac gtgggccttc gcgaggtcca cgacgatcag gtcggctcgc ttccccgtct
    17221 cgatcgaccc gacgaggcgg tcgagcccga gagccctcgc gccgtcgatc gtcgccatcc
    17281 ggaggagctc gggcgccgcg agcggcgtcg ggtcgagggc cgcgaccttc gcgagcttcc
    17341 ccgcgaagtc catggcgtcg aacatgtcga ggtcgttgtt cgagccggcc gagccgtcgg
    17401 tccccaggcc gacgaccacg cccgccttcc gggccgacgc gaccggggcg atccccgagc
    17461 cgagcttcat gttcgactcg gggttgtgcg aaaggctgac gccgcgcgcg gcgagtgtcg
    17521 cgaggtcctc cggcgaaagc cagacgccgt gggcgacgca gagccggggc cccaggaacc
    17581 cgacctcgtc gagccaggcc gtcgggctct tgccgtgctt ctccctcagc tgcgtctgct
    17641 cgtcccaggt ctccgagacg tgcgtcagga gcggggcgcc gtactcgtcg gcgagggccc
    17701 gggccgcctg cagcgtctcg cggctgcacg tgtagggggc gtgcggcgcg atcgccggcg
    17761 tgatccgcgg gtgccccttc cacttctcga ggaacgcgcg tgtcaccgcc ttcgtttcct
    17821 cgaggttcgc gtggccgggg gccgggaagt cgatccaggt ctcgccgagg actccgcgga
    17881 ggccggcgcg gtcgacggac gccgccacgt cggactcgaa gtagtacatg tcggcgaacg
    17941 tcgtcgtccc gccccggatc atctcgaggc aggcgaggtc ggtcccggca cgaacgaact
    18001 ccggggagac gttcttcccc tcggccggga agatgtactt cgtgagccac tccatgagcg
    18061 gctggtcgtt cgagaggccg cggaggaggt tcatcgcggc gtgcccgtgg gtgttcacga
    18121 ggcccgggag gaggacggcg tccttccgtt cgatccgctg cttcggcgcc cacgccgcgt
    18181 cgatcgccgc cgggtcgccg acggcgacga tctcgccggc ccggacggcg acggccgtcg
    18241 cagcgcgcac ggaaaaggcc gcgtccatcg ggagcgcgtg gcccgccacg acgacgaggt
    18301 cgcagggcgc cttcttcgcg ggggccttcg cggcgggcgc cgcggcggcc gggagggcga
    18361 ggaggagcgc ggcggcgaaa gcggtccgca cggtcgcgcc cctcacagct cgaaggccct
    18421 cggcaggagc gaggcgagcg tcgtctcctc cagcttcccc cccgtcgtcg ccatgacgac
    18481 ggcgatgtcg cggcactggt cccagaggac ctgccggcag gcaccgcagg gagaggtcgg
    18541 ctcgtccgcg cccgtcacga cggcgacggc ccggaacgaa cggacgcctt ccgagatcgc
    18601 cttgaagacc gccgtccgct ccgcgcagca ggtgaggccg tagctcgcgg attcgacgtt
    18661 gcagcctccg aaaatgcgtc cgtccgcggc gaggagcgcc gcgccgacgt ggaagtgcga
    18721 atagggggcg gacgcgttct cccgcgccgc cgtcgccgcg gcgacgagcg cctcgcgctc
    18781 ggccgccccg agggacctcg ccgtcatctc ctcacctcct ccgccgcgcc tgcggcttcg
    18841 cgccccggcg cagcccggct gccagcttcg tcatcacctt ccgcggcgct ttcttcgcgc
    18901 gccgggccgg ctcgacgacc ggccggtcgg acgatctccc cttccccatc gcctcgacca
    18961 gcgcggtgag gagcctgacg aagcggtcct tcacgcggcg cgtcgtctcg aggacctcgt
    19021 cgtggcggag cggctgcttc aggacgccgg ccgccatgtt cgtgatgcag gagacgccga
    19081 ggacgccgac gcccatgtgg gacagggcga tgacctcggg gacggtcgac atgccgacgg
    19141 cgctcgcgcc gagcgcccgg aacgcgcgga tctcggccgg cgtctcgtag gacggcccgg
    19201 tgacgccga
//
LOCUS       DAOONL010000068        19055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_2941_length_19055_cov_17.974211, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000068 DAOONL010000000
VERSION     DAOONL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19055
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2941_length_19055_cov_17.974211"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2861
                     /locus_tag="PLP50_10965"
     CDS             <1..2861
                     /locus_tag="PLP50_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA52111.1"
                     /translation="GPASTHPMASTPDALALVALFDTVQGFLWMKDLDGRYTYCNDLF
                     ARSFGGEAGGVLGKTDFDLFPAETAVAFQENDRKAIAAGGVVVFDEEVRLRRDDEKTL
                     FESRKGPLRDAGGRVVGTVGSSRAVADSKAAEDRLALEDRVLARIVAGEPLAGTLDAI
                     ARVIEAQTLDTAVTIYLLSACGNVLRLAAGPRVPEATLRVLDELPVGEKDSQSGAAAY
                     RKEAVAVEDVSTDPLWAGYRDVGLAHGIGAGFSTPLLSREGNVLGVLTLYLGRKGRLT
                     ERQRRLVDTAARIAAIAVARSREEQALRDSEARYRLVSEKSADVIWLYDLRAERFEWV
                     SPSIERLRGYTVAETLGRSLADVLTPESMALVAEQLPRALAEFAEKGEAAQTRTIEVD
                     QVRKDGSIVPTEVATTFVTDATGRVTHLQGVTRDISERRRAELARDEAAAFRDALIDA
                     SGDGIAVFDQEHRVVQTNSRFAEMLGYSREEVLSLRTWDFVADLTEEEVRIRFADLSR
                     VHETFESRHRRKDGSVYEVEVSASGLSVNGENVLFTISRDISARRQAEAARERLQQQL
                     LAAQKMESVGRLAGGVAHDFNNMLSVIQGHATLALDRVDPESPMAEDLREIRTAAQRS
                     ARLTRQLLAFARRQPTEPRALDLEETIGRMRRMLGQLLGEDVRLRIVSGTSAARVRID
                     PAQVDQLLTNLCTNARDAISGSGTVTIETACVVLDERWCARRGAGRPGRWVRLTVTDD
                     GCGIDPEVIPHVFEPFFTTKEVGRGTGLGLASVHGIVEQNGGFLDVESEPGRGASFHV
                     FLPRLEGSVSWSEHPAVRATPRGAGETLLLVEDEPALLGLVQRMLEGLGYRVLTAFCG
                     EEALEVAAENRSEIRLLVTDVVMPGMNGRELCERLLATVPGLRCLFVSGHPADVLADR
                     GLAGEGIRLLTKPFTAHDLAVQVREALDVSRPGGLTLD"
     gene            complement(2877..3569)
                     /locus_tag="PLP50_10970"
     CDS             complement(2877..3569)
                     /locus_tag="PLP50_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA52112.1"
                     /translation="MPAVLITGAGKGFGRAMLEEYLRRGWTAYPVVRDSRVAASLEGS
                     GCRPIVADVVASDAEGLIAATLDAYGDPLDLLVNNAGIIRKARGLAAAEPEDLESHFH
                     VHCLGALRCTRAARPWLRRAERPLVVNVTSRFGSIGQTAAGGFRGIYAYNVAKAAQNM
                     LTACLDQELRDERIRVVAVHPGRLRTTVGAADADTEPAVAARALADWVDRLDRDAPCA
                     CWDLMRGGVIEW"
     gene            3654..5012
                     /locus_tag="PLP50_10975"
     CDS             3654..5012
                     /locus_tag="PLP50_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52113.1"
                     /translation="MDAGHAEALPAGGGTVLARDLDGLKSVDDRWGHGAGDELPRHFA
                     SMMTAVVGPGRQTWRQRRVSLRKMSQGKTIPGKALPIRRSVIAQGGFRVGLARATVVA
                     LLASRGLALADERSLEPVLVAGGGTADGGTPVLVDLKDPESLAVDGQGRVYVADREKG
                     VIFVLDFRHGTAETITGPGSEQGRTSPGPASNRTLPGVQHIALDPFRGVFFAQEDVGV
                     VFRYDLVARTVERVAGRYSEAEFDGDGGPATKAVLRVHGLACDAEGNLYVSGANRIRR
                     ISRASGLIRTVAGTGKLGFWGDGGPATEADLANPGAMAFDRNGTLYFSDRANNRIRAI
                     DPKGRIRTVAGNGRSGPPEDGEATRSAAGEVVALAVDGDGDVVFVTEARFVRRLLVSR
                     NRLETFLQSPWSTLHYPREITGVAVGAEGSLYFDVPRSNGLMSLPAALLRKESGSKGS
                     RR"
     gene            5009..7057
                     /locus_tag="PLP50_10980"
     CDS             5009..7057
                     /locus_tag="PLP50_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52114.1"
                     /translation="MKACIVVALLLAGGAARAAGPTVVTVAGGGAGDGGPAVSAEVGA
                     QGGLVVLESGDVLFADPAHSSIRRVDAATRTISTVLRTGEREPPLSLALGAPGKVLFA
                     SAYRVEELDLATGHRRAIAGSGQSLYAYAPLELSGPATQVPLGHISAVAVDPQGRVCF
                     ADSFRGVILRVDPSTGSLERFFEIDGAPPTTGSEPAWFISGVAFDSAGALYFSDGRRH
                     LVHRVEAGKATAVAGTGEIRRAFPRKSAPPGQDDSAAPATSSPLIRPGPLAIRPTGEV
                     AFGEEYGLVQYVNRHGQLATLLGWKSRASAVGGIAFDRDGALYAKVTPESGVGQVLKL
                     PAGATEPEVLAGSGLAHCCGDGAPGPEAVLSKPEGVAIDAGGDLVFADTGNHRIRRVS
                     ARTGRIETIVGGGSYALPGSRHVNGHPIRPAPPPGRTHGLSFEVLWPRFVATDPAGNV
                     YFAQRDGPVHRVERDGTVVTLGAERKNALGAKFPDGFSGIGGLAVDASGTVFVAAEHR
                     IWRISADGRLGVLGGSGHEGFAGDGGFALPADLSRPAWPVVDGRGTLYFVDGGNSRIR
                     RIDRTGRISTVAGNGLGWELGEGLAIRQAIGTVTGLALDPAGDLHYATSDNRIWRLSR
                     ADGQLRVVAGRDFGRPPSPDGPVAGITSLGRPRALAFDRRGVLYFSEPDRSRVRAIRP
                     "
     gene            complement(7079..7915)
                     /locus_tag="PLP50_10985"
     CDS             complement(7079..7915)
                     /locus_tag="PLP50_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52115.1"
                     /translation="MSLASYVQKTYEADMEREIASGATNQGGRAPQELSFPPLPSVTY
                     LDPAFRLEARASSGLRVYFTASGDCSLSGSTVRILSAGSCVVTAQHPGNERYAPAPDV
                     VQVLRIRKADQWIIVPDLPFELPFGSGTLVAGMRASSGLDVVVRADGCCTADGASIRF
                     AGVGTCLITGHQPGSANYAAAPIVEHVVTVVKGEQTIQLPDLGERSHGPDDVPFVPVA
                     SSGLSVYISTAGPCVFTGEALRVTGTGACAITAEQPGNENYHPAPKAFVTVNVARPES
                     TW"
     gene            8586..12275
                     /locus_tag="PLP50_10990"
     CDS             8586..12275
                     /locus_tag="PLP50_10990"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52116.1"
                     /translation="MRLSTALALLLPAFVLASAAVGALPTATVTGSATICAGQTTEIR
                     ADLTGVGPFTVRWSDGFVDAGVVASASRHLVTPAATTTYTVTTVSDPGGSSAGTGSAT
                     VTVLPTAAPLTDITTPSPVAPGTTGLLASIPDAGAGATYAWTITNGSITDGQGTASIT
                     YSVGGLGKTLLGVTVASPGGCSATGATWTWVGADPAESLHVSRLAGPSGGGAWFDGPL
                     ATARFLNPSGVASDAAGNVYVLEWSWGLVRKVSPSGRVTTLAGLAGSYGAADGRGPIA
                     RFYYPDGIAVSASGEVYVADTGNHTIRRILPTGEVTLFAGTTGVLGGADGEPGVARFY
                     SPRGVVVDAAGTLYVADTSNHTIRKITPAGVVSTLAGEARSPGSANGNGSAARFRSPE
                     GVAVDAGGNVYVADTENHTIRKITPAGEVTTLAGMPGVTGGADGDATQALFRRPAALV
                     VDGTGNVLVADTGNMTVRLVSTSGVVSTLAGTALQRGNADGTGSDARFTSPSGIALEP
                     GGTFVVSDSRSVRRMTVAGVVSTVTGETDAPMGQVDGPGADARFYSPAALASDSHGNL
                     FVADSDAHVIRKVTPSGETTTFAGAVVDFPCALVDGPPADARFCSPSGLAIDALDNLY
                     VSDTYNNAIRKVTPDGVVTTLAGGSACESCDGTGRAAGFDEPWGLTVDPAGNVWVADF
                     YNHTIRRITPSGVVTTVVGRAGNLGYNDGTGSAASFDRPAGVAADRHGNVYVAEFESG
                     RIRKVTGDGVVTTLFGPHIMRPVGIQVDGSGNVYLSGYISTGILRVSPSGAATPVAGE
                     SVVPGAAEGTGAAALVHAVEGLALRPNGDLAFSNISARSIWQGVPSLADVATIDAISA
                     PVGQTRQLGVAPSTATSWHWELVRTESASTAALSATNVPNPTFTPDVPGLYKFRLTAS
                     DGTKKSVTIATLNAGLPAPSVHVTGGGAYCPGEAVPIRVESSGIPPLTITWSDGLVWS
                     GIHAGSGMREVTATTSTTITVTSVTDATGVTGVGTGGAVIEVTPRTATPVISAPATVG
                     AGSPDWTASIVPHPGNTYSWSISGGRITSGSHGSSITFTAGDAGTLTLYAREQAPGEC
                     QSSSGTATVQVLPAGSAMRFYPVTPCRVLDTRDASGPKFGQPLAGDGTLGLNVAGMCG
                     VSPTAKAVAANVTVTQPAGSGYIVVFPGDEPEPVTSTVHFTSGRTRASNTHLKLSLSG
                     LDDISIKNASPGPVHVILDVSGYYE"
     gene            12727..15957
                     /locus_tag="PLP50_10995"
     CDS             12727..15957
                     /locus_tag="PLP50_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52117.1"
                     /translation="MAGPAGARRLFVRYNYGFVTFDLGSPAAPTKLQTKDLRYNEAYP
                     VGGDGQTRAGMISASSDGARALVPWGGGGFGTIVLSPTPYSGGGEYLPTGGKTKATAA
                     LRTGDRYLAFSSNSSGALWVADVTTVARDVRANPENPNQIPSWSYTGPSYGGPLEILK
                     PIESASGAYLVAASETKVTVIDVSAPGPAGPGITSGFSHRTYTLSELGLSGSVRAVSA
                     ALHPIDGGLYLLAEGSTLGVGVLLSNGVSLTRIQPPATPGGQFGSAEAKGFYLPETGF
                     RRAMPGSVLLSFDTELVAVFVEGEGDEMGGPLHLQVRSSSNFGQNRAAGLEFTSPAQL
                     EALEGFRSTGGSFHLYMGLLGVGTYAASISCTLAPTPADGALSVEKIPATGAPVAVPD
                     NGTVFVGDELQIRPTFAPPDIVKPLTDWRFDYAFHEGDPTDASATTPFIVHSDIPLTP
                     GDYPSQLTLIGPCDPSQDAEQNPTSGAGCWDSVLASGDFATPDPAAGTTVQIPLAFEV
                     QNALNEGSSDLTKHRIQWKVPRPLLKSASILSGEYFEDASEGTPHRTSGTYRWYFASA
                     PVGQTGSDVLTLKADCTGPFCNPGLTQRGEYRYWLSVPYRNGFRTPDCPGLEGATCTV
                     PTAPTVSVTDVILGFSVPESGNVGDQVLTIGSTSKKGTATTPCGGTSGFTYDICTVSG
                     TSCPEGSYAGVGFIATKPFPDTGAGTVSIPMPQEGSWGVRIRYQYTTTGDCGNALTAK
                     WPDQGQWAPLTVIRSAPTVKLFNSTGTSELPKWHGTYSLATCQTARAYAYVDGVQEAA
                     LPSGASFTLTKIDDPPAGPNDLPISENQYASLSVSNRGDYVVTLRGYGVDATANIAAE
                     ALFGGGGGTPPPVISAPATVSAGSPGWTASIVPHPGSIYEWSILGGSITSGNDESSIT
                     FTAGDAGTLTLYAREQAPGECWSSQGTATVQVLPAASATRFYPVTPCRVLDTRDGSGP
                     TLGLPLASDGALGSDVAGRCGVSPTAKAVAANVTVTQPAGSGYIVVFPGDEPEPVTST
                     VHFTSGRTRASNTHLKLSLSGLDDISIKNASPGPVHVILDVSGYYE"
     gene            complement(16114..18381)
                     /locus_tag="PLP50_11000"
     CDS             complement(16114..18381)
                     /locus_tag="PLP50_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPA52118.1"
                     /translation="MRVPMSVRPVLVALFVLGGAAWGEEAAEAPPAIAKFTEKKFSHE
                     QYRAAILGKVAFRRGRKPVEPKEPLRVTFVRTDVKGAVVPIEVKDGEGFILQAVPGTW
                     EVREIARAGRLYRPGQQFQATPGNVVYIGFTDIALGDEDRSAYEYEYRLDTTVSMAAF
                     ARHPELGKLISAWAPASFFTNDNVSLFAPNYPVASGKVKTPKTLAAASEHGDLATARE
                     LLAAGADPDAPDSDGWTPLHSATRFGHEAIAGLLLDRGASPSTQNPNGWTPLMLALRY
                     GPEALAMRMLEKGASVERKAKDGWTPLMLAVRNDQPASARLLLQRGAAVNDRTKSGTT
                     ALMLAAGNAPEEIVTALLEKGATLAEKDGDGWSALTWALRYGKPGNARLLVERGADVQ
                     EVDGESWTPLMYALRYEVGDVAELLLDKGARPNERNKNGWSPLHFALRHGAPQVARRL
                     IESGAERDRPTSDGFTPLMLALRNGQGENAKLLIAKGVDVDTKSQDGWTPLMFALRYD
                     QAECGRLLARTSKRIDDKDKDGWTALMFALRYGNAEAAETLIQRGAAFNYTVAEGWEP
                     LLTALRNGQAENARRLIEKGANVKARTAQGWSALMFAIEYGQPENAKLLLRKGADGTL
                     PSTDGTTPVALAKKKGYFELVQLLGGDPAEMEPAHAKAAPARGLTYSQVVATYPAGVK
                     PCTTVVDVTGVSPTGQWQTLGIVEHRSHQPLLQYYGTRVTLKVPARAGGVNHPAGTLL
                     TVDADQNWIAVTSWD"
     gene            18813..>19055
                     /locus_tag="PLP50_11005"
     CDS             18813..>19055
                     /locus_tag="PLP50_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006458075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52119.1"
                     /translation="MALPEPGQLVRVRSRSWLVEEVVPPARPAEQTLVRLSCLDDDAQ
                     GDELAVLWEREVDAAVAGASGWTTVGKNGFDPTKLFS"
BASE COUNT         2756 a         6816 c         6632 g         2851 t
ORIGIN      
        1 ccgggccggc cagcacgcac cccatggcga gcacgcccga cgcgctcgcc ctcgtggcgt
       61 tgttcgacac cgtccagggg ttcctctgga tgaaggacct cgacggccga tacacctact
      121 gcaacgacct cttcgcgcgg tccttcggcg gggaggcggg tggtgtcctc gggaagaccg
      181 atttcgacct gttcccggca gagacggcgg tcgcgttcca ggagaacgac cggaaggcga
      241 tcgccgcggg cggcgtcgtc gtcttcgacg aggaggtgcg ccttcggcgg gacgacgaga
      301 agacgctctt cgagtcgcgg aaggggcccc ttcgcgacgc cgggggccgg gtcgtgggga
      361 cggtggggag ctcgcgcgcc gtcgccgact cgaaagccgc ggaagaccgg ctcgcgctcg
      421 aggaccgggt cttggcgagg atcgtcgccg gcgagcccct cgcggggacg ctcgacgcca
      481 tcgcgcgcgt catcgaggcg cagacgctcg acaccgcggt cacgatctac ctcctctcgg
      541 cctgcggaaa cgtcctccgg ctggccgccg ggccgagagt ccccgaggcg acgctgcgcg
      601 tcctcgacga gctcccggtc ggagagaagg attcccagag cggcgccgcc gcctaccgaa
      661 aggaagcggt cgcggtggag gacgtctcca cggacccgct ctgggccggc taccgcgacg
      721 tcggcctcgc gcacggcatc ggggcgggct tctcgacccc tctcctgagc cgcgagggga
      781 acgtcctggg cgtcctgacg ctctatctgg ggcgaaaggg gcgtctcacc gaacgccagc
      841 gacgtctggt cgacacggcg gcgcggatcg cggcgatcgc cgtggcgagg agccgcgagg
      901 agcaggcgct tcgcgacagc gaggcacgat atcggctcgt ctccgagaag tcggcggacg
      961 tcatctggct ctacgatctc cgcgccgagc gcttcgaatg ggtcagcccc tcgatcgaga
     1021 ggctccgcgg atacaccgtc gccgagacgc tcggccggtc gctcgccgac gtcctcacgc
     1081 ccgagtcgat ggcgctcgtc gccgagcagc ttccccgggc gctcgcggag ttcgcggaga
     1141 agggcgaggc ggcgcagacg cggacgatcg aggtggacca ggtccgcaaa gacggttcga
     1201 tcgtcccgac ggaggtcgcg acgacgttcg tcacggacgc gaccggacgc gtcacgcacc
     1261 tgcagggggt cacgagggac atctccgaga gaaggcgggc cgagctggcc cgcgacgagg
     1321 cggcggcgtt ccgggatgcg ctcatcgacg cctccggcga cggcatcgcg gtcttcgacc
     1381 aggagcaccg ggtcgtccag accaacagcc gattcgccga gatgctcggc tactcgcgcg
     1441 aggaggtcct ctcgctccgg acgtgggact tcgtggcgga cctgaccgag gaggaggtgc
     1501 gaatccgctt cgcggacctc tcgcgggtcc acgagacgtt cgagtcgcgg caccggcgga
     1561 aggacgggtc ggtctacgag gtggaggtct ccgcctcggg cctgagcgtc aacggcgaga
     1621 acgtcctctt caccatctcg cgggacatca gcgcgcgccg ccaggcggag gcggcgcggg
     1681 agcgcttgca gcagcagctg ctcgccgccc agaagatgga atcggtcggg cgcctggccg
     1741 gaggcgtcgc ccacgacttc aacaacatgc tttccgtcat ccaggggcac gcgaccctgg
     1801 ccctcgaccg ggtcgacccg gagagcccga tggccgagga cctgcgggag atccggacgg
     1861 ccgcccagcg gtcggcgcgg ctcacgcgcc agctcctcgc gttcgcccgg cggcagccga
     1921 ccgagccgcg cgccctggac ctcgaggaga cgatcggccg gatgcgccgg atgctcggcc
     1981 agctcctcgg cgaggacgtc cgcctccgga tcgtctccgg cacgagcgcc gcgcgggtcc
     2041 gcatcgaccc ggcccaggtc gaccagctcc tgacgaacct ctgtacgaac gcgcgcgacg
     2101 cgatctccgg ctcgggaacc gtcacgatcg agaccgcctg cgtcgtgctc gacgagcggt
     2161 ggtgtgcgag gcgcggcgcg ggccgccccg ggcgctgggt gcggctcacg gtcacggacg
     2221 acggctgcgg catcgacccg gaggtgatcc cccacgtctt cgagcccttc ttcaccacga
     2281 aggaggtggg ccgggggacc ggcctcggcc tcgcctcggt ccacggcatc gtggagcaga
     2341 acggcgggtt cctcgacgtg gagagcgaac ccgggagggg agcgagcttc cacgtcttcc
     2401 tcccccggct cgaagggtcc gtctcctggt cggagcaccc cgcggtccgt gcgactccgc
     2461 ggggggccgg agagaccctc ctcctcgtgg aggacgagcc ggcgctcctc gggctcgtgc
     2521 agcggatgct cgaggggctc ggctatcgcg tcctcaccgc gttctgcggc gaggaggcgt
     2581 tggaggtcgc ggcggaaaac cgttcggaga tccggctcct cgtcaccgac gtcgtgatgc
     2641 ccgggatgaa cggccgcgag ctctgcgagc gtctcctcgc gacggtcccg ggcctccggt
     2701 gcctcttcgt ttcgggtcac ccggccgacg tcctcgcgga cagggggctc gccggcgaag
     2761 gtatccgcct cctgacgaag cccttcacgg cccacgacct cgccgtccag gtccgcgagg
     2821 ccctcgacgt ctcccggccc ggaggtctca cgctcgactg agcggcgaag agcgcgtcac
     2881 cattcgatga cgccgccgcg catcaggtcc cagcaggcgc acggggcgtc gcggtccagc
     2941 cggtcgaccc agtcggcgag cgcccgagcg gccacggcag gctccgtgtc ggcgtccgcc
     3001 gcccctaccg tggtccgaag ccgccccggg tgaacggcga cgacccggat gcgctcgtcg
     3061 cgaagctcct ggtccaggca ggccgtcagc atgttctggg cggccttggc cacgttgtag
     3121 gcgtagatcc cgcggaaccc gccggccgcg gtctgtccga tggacccgaa tcgagacgtg
     3181 acgttcacca ccagaggccg ctccgccctc cggagccacg gtcgcgcggc ccgcgtgcat
     3241 cgcagcgccc cgaggcagtg gacgtggaag tggctctcga ggtcctcggg ctcggccgcc
     3301 gcgaggccgc gggcctttcg gatgatcccg gcgttgttca cgaggaggtc cagcggatca
     3361 ccgtaggcgt cgagggtggc cgcgatcaga ccctcggcgt cgctcgcgac gacgtcggcg
     3421 acgatgggac ggcagccgga accctccagc gacgcggcga cccgggagtc gcgaacgacg
     3481 ggataggccg tccatccgcg ccggaggtac tcctccagca tcgcccgtcc gaagcccttc
     3541 ccggcgccgg taatcaggac cgctggcatg tgtggtctcc catgaacctt acgtggcgtc
     3601 tgcggaaagg gtctgcgcga tcgcgtcaga cggcccggct cgagttccgc cggatggacg
     3661 caggacacgc cgaggcgctg ccggccggcg gcgggacggt gttggcgcgc gacctcgacg
     3721 ggctgaagag cgtggacgac cgatggggtc acggggccgg cgacgagctc ccgcgccatt
     3781 tcgcctcgat gatgacggcg gtcgtcggcc cggggagaca gacgtggcgg cagcgaagag
     3841 ttagcctacg gaagatgagt caggggaaga cgatccccgg aaaagcgttg ccaattcgcc
     3901 gttcggtcat tgcgcagggt ggctttcgcg tcggattggc ccgggccacc gtcgtcgctc
     3961 tcctcgcctc ccgcggcctc gccctggcgg acgagcgatc gctcgagccg gtcctcgtgg
     4021 cggggggcgg aaccgccgac gggggcacgc ccgtgctcgt ggatctgaag gaccccgagt
     4081 cgctcgccgt cgacgggcag ggccgcgtct acgtagccga ccgcgagaag ggcgtgatct
     4141 tcgtcctcga tttccggcac ggaacggcgg agaccatcac cgggccggga tccgagcagg
     4201 gccgtacgtc gccgggcccg gcgtcgaacc ggacgctgcc cggcgtccag cacatcgcgc
     4261 tcgacccctt ccgaggggtc ttcttcgcgc aggaggacgt cggggtcgtc tttcggtacg
     4321 acctcgtcgc gcggaccgtg gagcgtgtcg ccgggaggta cagcgaggcc gagttcgacg
     4381 gagacggagg gccggcgacc aaggcggtgc ttcgcgtgca cggtctcgcc tgcgacgccg
     4441 aggggaacct ctacgtctcg ggagcgaacc gcatccggcg catctcacgc gcctccggtc
     4501 tgatccggac cgtggccggg acggggaagc tcggattctg gggcgacggg ggaccggcga
     4561 ccgaagccga cctcgcgaac cccggggcca tggcgttcga tcggaacggg acgctctact
     4621 tctccgacag ggcgaacaac aggattcgcg cgatcgaccc gaaggggagg atccggaccg
     4681 tcgcgggcaa cggcagaagc gggccgccgg aagacggcga agcaacccgc tccgcggcgg
     4741 gcgaggtcgt cgctctcgcc gtggacggcg acggagacgt cgtcttcgtg accgaggcgc
     4801 gattcgttcg tcggcttctg gtctcgcgga accggctgga gacgttcctt caaagcccgt
     4861 ggtccacgct ccactaccct cgagagatca cgggtgtcgc tgtcggggca gaaggctcgc
     4921 tctacttcga cgtcccgagg tcgaacgggc tgatgtccct gccggccgcc cttctccgta
     4981 aggagagcgg ctcgaagggc tcccggcggt gaaggcctgc atcgtcgtcg cccttctgct
     5041 ggccggcggg gccgcgcgcg ctgccggccc cactgtcgtc acggtggcgg gaggcggagc
     5101 cggcgacggc ggcccggcgg tgagcgcaga ggtcggagcg cagggcgggc tcgtcgtcct
     5161 cgagtcgggt gacgtgctgt tcgccgaccc cgcgcattcg agcatccgga gagtcgacgc
     5221 cgcgacccgg acgatctcga cggtcctgcg gacgggggag cgagagccgc ccctctctct
     5281 cgcgctggga gcgccgggga aggtcctctt cgcctccgcc taccgggtgg aggagctcga
     5341 cctcgccacg gggcaccgca gagcgattgc gggaagcggc caatctctgt acgcatatgc
     5401 gccgctcgag ctgagcgggc cggcgaccca ggtcccgctg ggccacattt cggccgtcgc
     5461 cgtcgacccg cagggtcgcg tctgctttgc ggactcgttt cgaggagtga tcctccgcgt
     5521 cgatccttcg acggggtccc tcgagcggtt cttcgagatc gacggagccc cgccgacgac
     5581 cggctccgag cccgcctggt tcatctccgg cgtcgcgttc gactctgccg gagccctcta
     5641 tttctccgac ggccgccgcc acctggtcca tcgggtcgag gccggcaagg cgacggccgt
     5701 cgccgggacc ggggagatcc gaagggcctt ccccaggaag tcggcccctc ccggccagga
     5761 tgacagcgcg gcgccggcca cgtcgtcgcc gctcatccgg cccgggccgt tggctattcg
     5821 accgacgggc gaggtcgcct tcggagagga gtacggcctg gtccagtacg tcaaccgtca
     5881 cggccagctc gcgacgctcc tcggctggaa gtcgcgcgct tcggccgtcg gagggatcgc
     5941 tttcgacagg gacggcgcgc tctacgcgaa ggtcactccc gagagcggcg tggggcaggt
     6001 gctgaagctg cccgccggtg ccacggagcc ggaggtcctg gcgggaagcg gtctcgcgca
     6061 ctgctgcggg gacggcgccc cgggcccgga ggcggtgttg tcgaagcccg aaggggtagc
     6121 gatcgacgcg gggggcgacc tcgtcttcgc ggacaccggg aatcaccgca ttcggcgagt
     6181 ctctgcccga acgggccgga tcgagacgat cgtgggcggc gggagctacg cgctgcccgg
     6241 gagccgtcac gtgaacggac acccgatccg ccccgccccc ccgccgggga ggacccacgg
     6301 cctttcgttc gaggtgctct ggccgcgatt cgtggcgacg gaccctgccg gcaacgtcta
     6361 cttcgcccag cgcgacgggc ccgtgcaccg ggtcgagcgg gacgggaccg tcgttacgct
     6421 cggcgcggag cgaaagaacg cgctgggagc gaagtttccg gacggtttct ccggcatcgg
     6481 cggcctcgcg gtcgacgcgt cgggcaccgt cttcgtcgcg gcggagcatc ggatctggag
     6541 gatctccgcc gacggccggc tgggcgtcct cggcggatcg gggcacgagg ggttcgcggg
     6601 agacggcggc ttcgcgctcc ccgccgacct ctcgcggccc gcctggcccg tcgtcgacgg
     6661 ccgaggcacg ctctacttcg tcgacggggg caacagcagg atccgccgga tcgaccggac
     6721 cggcaggatc tcgacggtcg cggggaacgg cctcggctgg gagctgggag agggcctggc
     6781 gattcggcag gcgatcggga ccgtgacggg cctggcgctc gatcccgccg gagacctcca
     6841 ctacgcgacg agcgacaacc ggatctggcg cctttcccgg gcggacgggc agctgcgggt
     6901 cgtggccggc cgggacttcg gacgcccgcc ttcgcccgac ggaccggtcg ccggcatcac
     6961 gagcctgggc cggcctcgcg cgctcgcgtt cgaccgccgc ggagtcctct atttctccga
     7021 gcccgacagg tcccgggtga gggccatccg gccgtaggcc gcgttcgcgg cggggtgatt
     7081 accaggtcga ctccgggcgg gccacgttca ccgtcacgaa ggccttcggc gcggggtggt
     7141 agttctcgtt ccccggctgc tcggcggtga tcgcgcaggc acccgtgccg gtgacccgga
     7201 gcgcctcgcc cgtgaagaca caggggcccg cagtcgagat gtacacggag agacccgagg
     7261 aggcgacggg gacgaacggg acgtcgtccg gaccgtgcga gcgttccccg aggtcgggta
     7321 gctggatcgt ctgctctccc ttcacgactg tcacgacatg ctcgacgatc ggagccgccg
     7381 cgtagttcgc cgagcccggc tggtggccgg tgatcaggca ggtcccgact cccgcgaacc
     7441 ggatgctcgc tccgtccgcc gtgcagcagc cgtctgccct cacgacgacg tcgagaccgg
     7501 acgaggcgcg catcccggcg acgagcgtcc cgctcccgaa ggggagctcg aatgggaggt
     7561 cgggcacgat gatccactgg tccgccttcc ggatccggag gacctggacg acgtccgggg
     7621 ccggtgcgta tctctcgttg ccgggatgct gagcggtgac gacgcagctc cccgcggaga
     7681 ggatcctcac ggtggagccg ctgagcgagc aatcgccgga agcggtgaag tagacgcgga
     7741 gccccgagct cgccctggct tcgagccgga aggcgggatc gaggtaggtc acggaaggga
     7801 gcggcggaaa cgacagctcc tggggcgccc tcccgccctg gttcgtcgcg cccgaggcga
     7861 tctccctctc catgtcggct tcgtaggtct tctgaacgta ggaggcgagg ctcacgaagg
     7921 cggcctctcc gagacgcgac cggagcagcg cgacgcgccc gtcgagcagt cgatccctct
     7981 cgtccggcgg cgcgctccgg gcgaggcgag agtccgcgag cgcccaatcg cgcgcgacct
     8041 cccggtagat cggcccctcc gattcgagga gatacgccgc gcgctcgtcg gcgggagtgg
     8101 agttccggag gaagaacctc caggtcgctc gcgaaggcga tggcggctgc gtagcctgcg
     8161 cagcccgata gcgcgaaagg tatccgccgc ccccgggcgc agggaatcct ccgccgactc
     8221 ccggaggagg cccgacccgt tcgctcccgc gtacaactgt gccgccggtc cacgcccggg
     8281 gttccgtctg ggcattggca ccgacggaca gagcgacccc ggacgccaga gcgaggagcg
     8341 caacgacggg cttcatgcga atagacgagt agcaaccctc gtaccgcccg tgtcgtctcc
     8401 tcgcaaggac aacgagatgg ggccggctcc tgctcattga ggacctcgat cggaccagct
     8461 gtttcgagct tgtcgcttcg gtggctgccg cgtaggcttc caaggcgact gaccgtctcc
     8521 ccggggcgga gtccgacaat cggtgggata cgccccgccc gacgaggagc tcctggatga
     8581 aacctatgcg actttcgacc gcgctcgccc ttctccttcc ggccttcgtc ctcgcgtccg
     8641 ccgccgtcgg ggccctcccg acggcgacgg tcacggggtc ggcgacgatc tgcgcgggcc
     8701 agacgacgga aatccgggcc gacctgacag gcgtcggtcc cttcaccgtc cgatggtccg
     8761 acggcttcgt cgacgccgga gtcgtcgcga gcgcctcgcg ccacctcgtc accccggccg
     8821 cgacgacgac ctacaccgtc accaccgtca gcgatccggg aggctcgagc gccggcaccg
     8881 ggtcggccac ggtcaccgtc ctcccgaccg cggcgcccct caccgacatc acgacgccga
     8941 gccccgtcgc gccggggacg acgggccttt tggcgagcat cccggacgcc ggggcgggcg
     9001 ccacctacgc ctggacgatc acgaacgggt cgatcacgga cggccagggg acggcgtcga
     9061 tcacctactc cgtcggcggc ctcggcaaga cgctcctcgg cgtcaccgtc gcatctccgg
     9121 gcggctgctc ggcgaccgga gcgacgtgga cctgggtcgg cgccgacccg gccgagtcgc
     9181 tccacgtgtc gcgcctcgcc gggccgagcg ggggaggcgc ctggttcgac gggccgctgg
     9241 cgacggcgcg cttcctcaat ccgtcggggg tcgcgagcga cgccgccgga aacgtctacg
     9301 tcctcgagtg gtcgtggggg ctcgttcgga aggttagccc gtccggccgc gtcaccacgc
     9361 tcgcgggcct cgcgggctcg tacggggcgg ccgacggacg cggcccgatc gcccgcttct
     9421 actacccgga cgggatcgcg gtctcggcct ccggcgaggt ctacgtcgcc gacaccggca
     9481 accacacgat ccgacgcatc ctccccaccg gcgaagtcac cctcttcgcc gggacgaccg
     9541 gggttctcgg aggcgccgac ggcgaaccgg gagtcgcgcg gttctactcg ccccgaggtg
     9601 tcgtcgtcga cgcggccggg actctctacg tcgcggacac gagcaaccac acgattcgga
     9661 agatcacgcc ggctggtgtc gtgtcgacgc tcgccgggga ggcccgatcg cccgggagcg
     9721 cgaacggcaa cggcagcgcg gctcgattcc ggagtccgga gggcgtggcc gtcgacgcag
     9781 gcgggaacgt ctacgtcgcg gacacggaaa accacacgat ccgaaagatc acgccggctg
     9841 gcgaggtcac gacgctggcc gggatgcccg gggtcacggg gggcgccgat ggcgacgcga
     9901 cccaggccct gttccgcagg ccggccgcgc tcgtcgtcga cggcacgggg aacgtcctcg
     9961 tggccgacac cggcaacatg accgtccggc tcgtctcgac ctccggcgtc gtttcgacgc
    10021 ttgccggaac cgccctgcaa cgcgggaatg cggacggaac gggctccgac gctcgcttca
    10081 cctctccgag cggaatcgcc ctcgagccgg ggggcacctt cgtcgtttcc gacagccgct
    10141 ctgtccggag gatgacggtc gccggcgtcg tctcgaccgt caccggcgag accgacgccc
    10201 cgatgggcca ggtcgacggc ccaggggccg acgcccggtt ctactctccc gcggccctcg
    10261 cctccgactc tcacggcaac ctcttcgtcg ccgactccga cgcccacgtc atccggaagg
    10321 tcacgccctc gggcgagacg acgaccttcg cgggcgccgt cgtcgacttt ccctgcgcgc
    10381 tcgtcgatgg gccgcccgcg gacgcgaggt tctgctcccc atcgggcctc gcgatcgacg
    10441 ccctggataa cctctacgtc tccgacacct acaacaacgc gattcgcaag gtgacgcccg
    10501 acggcgtcgt gacgacgctc gctgggggga gcgcctgcga atcgtgcgac gggacaggcc
    10561 gggcagcggg cttcgacgag ccctggggcc tgacggtcga ccccgccggc aatgtctggg
    10621 tcgccgattt ctacaaccac accatccgcc ggatcacgcc ttcgggcgtc gtcacgaccg
    10681 tcgtcgggag ggccgggaac ctgggctaca acgatggaac cggaagcgcc gcgagcttcg
    10741 atcgcccggc cggcgtggcg gcggatcgcc atgggaacgt ctacgttgcc gaattcgaga
    10801 gcgggaggat caggaaggtg acgggcgacg gcgtcgtcac aaccctcttc gggccgcaca
    10861 tcatgaggcc ggtcggaatc caggtcgacg gatcggggaa cgtctaccta tccggctaca
    10921 tcagcaccgg gattcttagg gtatctccct cgggagccgc gaccccggtc gctggcgaga
    10981 gcgttgtgcc tggcgcggct gaagggacgg gcgccgcggc gctcgtccat gcggtcgaag
    11041 ggctcgctct ccgcccgaac ggcgacctcg ctttctccaa catctcggcg aggtcgatct
    11101 ggcagggtgt tccgtcgctc gccgacgtgg cgacgatcga cgccatctcg gctcccgtcg
    11161 gccagacacg tcagctcgga gtcgccccga gcaccgcgac gagctggcat tgggagctcg
    11221 tccggaccga gtcggcctcg acggccgccc tgtccgccac gaacgtcccg aacccgacgt
    11281 tcacgccgga cgtccccggc ctctacaagt tccgtctcac ggcctccgac ggaacgaaga
    11341 agagcgtcac catcgccacg ctcaatgccg gccttccagc gccgagcgtc cacgtgacgg
    11401 gaggcggcgc ctactgccct ggcgaagcgg tccccattcg cgtggagagt tcgggcatcc
    11461 cgcccctgac gatcacctgg tcggacggcc tcgtttggag cggaatccat gcggggtccg
    11521 ggatgaggga ggtcacggcc accacttcca cgaccatcac cgtcacctcg gtcactgacg
    11581 ccaccggcgt gaccggcgtc ggtacgggcg gcgccgtgat cgaagtgacg ccgcggaccg
    11641 ccaccccggt catctccgct cccgccaccg tcggcgccgg gtcgcccgat tggacggcct
    11701 ccatcgttcc ccatcccgga aacacctact cctggtcgat ctcgggtgga aggatcacgt
    11761 cggggagcca cgggagctcg atcacgttca cggcaggcga tgccggaacc ctcacgctct
    11821 acgcgaggga gcaggccccc ggcgagtgtc agtcgtcgtc gggcaccgcg acggtccagg
    11881 tcctccccgc aggctcggcg atgcggttct atccggtgac gccctgccgc gtcctcgaca
    11941 cacgcgacgc ctccggcccg aagttcggcc agcccctcgc gggcgacggc acgctcggcc
    12001 tcaacgtcgc ggggatgtgc ggagtctcgc cgacggcgaa ggccgtcgcc gcgaacgtca
    12061 ccgtcaccca gccggccggg tcgggctaca tcgtcgtctt cccgggagac gagcccgagc
    12121 cggtcacgtc gacggtccac ttcacttccg ggcgcaccag ggcctcgaac actcacctga
    12181 agctctcgct gtcgggcctc gacgacatct ccatcaagaa cgcgagcccc ggccccgttc
    12241 acgtcatcct cgacgtcagc gggtactacg agtagcggcg tcgccaccga acgatcggga
    12301 cagggggcgg cggccaccgg ccgccgcccc ctgtctcgat tgctttccga gacgcgaaag
    12361 atccggcccg cgaacgacac tacctccgga tgcttgtcgc cccgcctcag acgagacgcg
    12421 ctctctcccg cagcgtcatt tcctgcgcct gtcccataat ccaccttgct ctcttcgcct
    12481 gccgcggccg gtgagcgcgg tgaggtatct gttttcttgg ggtgccgacc acggggggac
    12541 tcgatgaacg tagctcgcga aggttcgaac ggacgtcttc ttccggctct gatcgctctc
    12601 gtcgccgccg cggggagcct cgcttcggca cgcgccgagg cgcagtgcca ggtcgtgagc
    12661 cctccctcgc tcttcgatcc ggtcgtcccg ggtccgagtt tcgttcccca gccgaacagg
    12721 ctcgccgtgg ctggcccggc cggagcgagg cggctcttcg tccgatacaa ctacgggttc
    12781 gtcaccttcg acctcggctc tccggcggcg ccgacgaagc ttcagacgaa agacctccgg
    12841 tacaacgaag cgtatcccgt cggcggagat ggtcagaccc gcgccggcat gatctcggcc
    12901 tcgtccgacg gggcgcgagc tctcgtcccc tgggggggcg ggggattcgg gacgatcgtc
    12961 ctgagcccga cgccgtactc cggcggcggt gagtacctgc cgaccggcgg gaagacgaag
    13021 gcgacggcag ccctgaggac gggcgatcga tacctggcct tctcgtcgaa ctcgtccggg
    13081 gctctctggg tggccgacgt gacgaccgtc gcgcgcgacg tcagggcgaa cccggagaac
    13141 ccgaaccaga tcccgtcctg gtcgtacacg ggtccctctt acggcggccc gttggagatc
    13201 ctgaagccca tcgagagtgc ctccggcgcg tacctcgtcg ccgcctcgga aacgaaagtc
    13261 accgttatcg acgtctccgc tcccggcccg gccggcccgg gaatcacgag cggcttctcc
    13321 caccgcacgt acacgctctc cgagctcggc ctctccggga gcgtgagggc cgtctccgcc
    13381 gctctccacc cgatcgacgg cgggctttac ctccttgccg aggggtcgac cctcggcgtc
    13441 ggcgtcctcc tgtcgaatgg cgtgtcgctg acacgcattc agccgccagc gactcccggc
    13501 ggccagttcg gttccgccga agccaagggc ttctacctgc cggagacggg cttccggcga
    13561 gcgatgcccg gcagcgtcct gctctcgttc gacacggagc tcgtggccgt cttcgtcgaa
    13621 ggcgaggggg acgagatggg tggcccgctc caccttcagg ttcgttcgtc gagcaacttc
    13681 ggccagaacc gcgccgcggg cctcgagttc acctcgccgg cccagctcga ggcgctggaa
    13741 ggtttccgct ccaccggggg atcgttccac ctctacatgg gtctcctggg cgtagggacc
    13801 tacgcggcgt cgatctcctg cacgctggcc ccgacgcccg ccgatggggc gctgtcggtc
    13861 gagaagatcc cggcgactgg tgcgccggtg gccgttcccg acaacggcac ggtcttcgtg
    13921 ggcgacgagc tccagatcag gcccaccttc gctcctcccg acatcgtcaa gcccctcacc
    13981 gactggcgct tcgactacgc gttccacgag ggggacccga ccgacgccag cgcgacgaca
    14041 ccgttcatcg tgcactcgga catccccttg actccgggcg actatccgtc gcagctgacg
    14101 ctgatcggtc cctgcgaccc ctcgcaggac gcggagcaga acccgacgag cggcgccggg
    14161 tgctgggatt ccgtcctcgc gagcggcgac ttcgctaccc ccgatcccgc ggccggaacc
    14221 accgtccaga ttcccctcgc ttttgaggtc cagaacgcgt tgaacgaggg cagctccgac
    14281 ctcacgaagc accgaatcca gtggaaggtc cccaggccgc tgctgaagag cgcctcgatc
    14341 ctctcgggcg agtacttcga ggacgcctcc gaagggacgc cgcaccggac gagcggcaca
    14401 taccggtggt atttcgcgtc ggcgcccgtg ggccagaccg ggagcgatgt cctgaccctg
    14461 aaggccgact gcaccggccc gttctgcaac ccgggcctca cgcagcgcgg cgaataccgc
    14521 tactggctct ccgttcccta ccggaacggc ttccgaacgc ccgactgccc gggtctcgaa
    14581 ggagccacct gcaccgttcc gacggcgccg accgtctccg tcacggacgt catcctcggc
    14641 ttctcggtcc ccgaaagcgg gaacgtcggc gatcaggtgc tgacgatcgg cagcacgtcg
    14701 aagaagggga ccgccacaac gccctgtgga gggacgagcg gcttcaccta cgacatctgc
    14761 accgtgagcg gaaccagctg ccccgagggg agctacgccg gggtcggctt catcgcgacg
    14821 aagccgttcc cggacacggg ggccggtacc gtcagcattc cgatgccgca ggaaggatcc
    14881 tggggcgtcc ggatccgata ccagtacacg acgactggcg actgcgggaa cgcgctgacc
    14941 gcgaagtggc ccgaccaggg gcagtgggcg ccgctcacgg tcatccggtc ggctccgacc
    15001 gtcaagctct tcaactcgac ggggacgagc gagcttccca agtggcacgg cacatactcg
    15061 ctcgccacgt gtcagacggc acgcgcctac gcctacgtcg acggtgtgca agaagccgcc
    15121 ctcccgtccg gcgcctcgtt caccctgacg aagatcgacg acccccccgc cggcccgaac
    15181 gacctgccga tttcggagaa ccagtacgcg agcctctccg tttcgaaccg cggtgactac
    15241 gtcgtcaccc ttcgcggcta cggcgtcgac gccacggcga acatcgccgc cgaggcgctc
    15301 ttcggaggcg gcggagggac gccccctccg gtcatctccg ctcccgccac cgtcagcgcg
    15361 gggtcgccgg gctggacggc ctccatcgtc ccgcaccccg gaagcatcta cgagtggtcg
    15421 atcttgggag ggagcatcac gtcggggaac gacgagagct cgatcacgtt cacggcaggc
    15481 gatgccggca cgctgaccct ctacgcaaga gagcaggccc ccggcgagtg ttggtcgtcg
    15541 cagggcaccg cgacggtcca ggtcctcccc gcggcctcgg cgacgcggtt ctatccggtg
    15601 acgccctgcc gcgtcctcga cacgcgcgac ggctccggcc cgacgctcgg cctgcccctc
    15661 gcaagcgacg gcgcgctcgg cagcgacgtc gcggggaggt gcggcgtttc gccgacggcg
    15721 aaggccgtcg ccgcgaacgt caccgtcacc cagccggccg ggtcgggcta catcgtcgtc
    15781 ttcccgggag acgagcccga gccggtcacg tcgacggtcc acttcacttc cgggcgcacc
    15841 agggcctcga acactcacct gaagctctcg ctgtcgggcc tcgacgacat ctccatcaag
    15901 aacgcgagcc ccggccccgt tcacgtcatc ctcgacgtca gcgggtacta cgagtagcgg
    15961 tatcgccacc gggcggtgag gggtcgagtc ggccgcttcg tcgccgccga cgtggacccc
    16021 ggaagggcgg ccgacgcggg cctccacgtg cgtcgcccgg agggccttcg attcgtcgac
    16081 ccgagatgcg atccggcgaa ggccttccgg gatctagtcc cagctcgtca cggcgatcca
    16141 gttctggtcc gcgtcgacgg tcaggagcgt cccggcgggg tgattcacgc ccccggccct
    16201 ggccgggacc ttcagcgtca cgcgggtgcc gtagtactgg agcagcggct ggtggctgcg
    16261 gtgctcgacg atcccgagcg tctgccactg gccggtgggg gagacgcccg tcacgtcgac
    16321 gaccgtcgtg cacggcttga cgcccgccgg gtaggtcgcg acgacctgcg agtaggtgag
    16381 gcccctggcc ggcgccgcct tcgcgtgggc cggctccatc tccgcgggat cgccgccgag
    16441 cagctggacg agctcgaagt accccttctt cttcgcgagg gcgacgggcg tcgtcccatc
    16501 cgtgctcggc aacgtgccgt cggctccctt ccgtaggagc agcttggcgt tctcgggctg
    16561 cccgtactcg atcgcgaaca tcagcgccga ccagccctgg gccgtccgcg ccttgacgtt
    16621 cgcccccttc tcgatcaggc ggcgggcgtt ctcggcctgg ccgttgcgca gcgcggtgag
    16681 gagcggctcc cacccctccg ccaccgtgta gttgaaagcg gcgcctctct ggatgagtgt
    16741 ctccgccgcc tcggcgttcc cgtagcgcag ggcgaacatg agcgccgtcc agccgtcctt
    16801 gtccttgtcg tcgatccgct tgctcgtccg ggcgagaagc cggccgcatt cggcctggtc
    16861 gtaccggagc gcgaacatca gcggcgtcca gccgtcctgg ctcttcgtgt cgacgtcgac
    16921 gcccttcgcg atgaggagct tcgcgttctc gccctggccg ttccgaagag cgagcatcaa
    16981 aggcgtgaag ccgtcggagg tggggcggtc cctctcggca cccgactcga tgagccgtcg
    17041 cgccacctgc ggcgcgccgt gacggagcgc gaagtggagc ggcgaccagc cgttcttgtt
    17101 cctctcgttc ggccgcgcgc ccttgtcgag gagaagctcg gccacgtccc cgacctcgta
    17161 ccgaagggca tacatcagcg gcgtccagct ctcgccgtcc acctcctgga cgtccgcccc
    17221 cctctccacg agcagccggg cgttcccggg ctttccgtag cggagggccc aggtcagggc
    17281 cgaccacccg tccccgtcct tctcggccag ggtggcgccc ttctcgagga gggccgtcac
    17341 gatctcctcg ggggcgttcc ccgcggcgag catcagggcc gtcgtcccgc tcttcgtccg
    17401 gtcgttcacg gcggcgcctc tctggagcag gagccgcgcg ctcgcgggct ggtcgttgcg
    17461 gacggcgagc atcaggggag tccagccgtc cttcgccttc cgttcgaccg aggcgccctt
    17521 ctcgagcatc cgcatggcca gcgcttccgg cccgtaccgc agcgccagca tcagcggcgt
    17581 ccagccgttc gggttctgcg tcgagggcga ggctccgcgg tcgaggagca ggcctgcgat
    17641 cgcttcgtgg ccgaagcgcg tggccgagtg gagcggcgtc cagccgtcgg agtccggggc
    17701 gtccgggtct gcgcccgcgg cgaggagctc gcgcgccgtg gcgaggtctc cgtgctcgga
    17761 agccgcggcg agcgtcttcg gcgtcttcac ttttccggag gcgacggggt agttgggggc
    17821 aaagagcgac acgttgtcgt tcgtgaagaa cgaggcgggc gcccaggcgc ttatcagctt
    17881 gcccagctcc gggtgacgcg cgaacgccgc catgctgacc gtcgtgtcga ggcgatactc
    17941 gtattcgtat gcggaccggt cctcgtcccc gagcgcgatg tcggtgaagc cgatgtagac
    18001 gacgttcccg ggcgtcgcct ggaactgctg gcccggcctg tagaggcgcc cggctcgggc
    18061 gatctcccgt acctcccagg tgccgggaac ggcctgcagg atgaagccct cgccgtcctt
    18121 cacctcgatc ggaacgacgg cccccttcac gtccgtcctg acgaacgtca cgcgcagcgg
    18181 ctctttcggc tcgacgggct tgcgtcccct ccggaacgcg accttcccga ggatggcggc
    18241 gcggtactgc tcgtgcgaga acttcttctc ggtgaacttc gcgatcgccg gtggcgcctc
    18301 ggcggcctct tcaccccacg cggccccgcc cagcacgaac agggcgacga ggaccggacg
    18361 cacgctcatc ggtacccgca tggactcctc ctgccgcctc ccggcgttgt cggacgatcg
    18421 aacggagaat tattgcatcg cagcagaaga ccccggtgac gtggggagga tgcggggggg
    18481 gtcgatgcag gtcggcgagc agctcgcgtc gggcgcgaac gtccgcaggg tcccggtggg
    18541 ctcgcccgcc gggaacgccc ccttcgtcgc tccgccgccg aggaccagag cgttggccag
    18601 gcccttctcc cccttctccg cacctggagc gaagtggcct tcctcgctcc gcctcgaagg
    18661 cgaagaggac tgaggccatc cgcctcaccc gaatggggga tgacggctcc gggacaggcg
    18721 tctagcctgc aaaggagtac cctccaggag tcccacggcg gaggaggccc ggcaaaccgg
    18781 tggccgaatt ggcggaaacg tgtgtcgcgc cagtggcgct tcccgagccc ggccagctcg
    18841 tgagggtgcg gtcgcgctcg tggctcgtcg aggaggtcgt cccgcccgcc cgtcccgccg
    18901 agcagaccct cgtccgcctc tcctgcctcg acgacgacgc gcagggcgac gagctggccg
    18961 tcctctggga gcgcgaggtc gacgcggctg tcgcgggcgc ctcgggctgg acgacggtgg
    19021 ggaagaacgg cttcgacccc acgaagctct tctcg
//
LOCUS       DAOONL010000069        18153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3187_length_18153_cov_15.778318, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000069 DAOONL010000000
VERSION     DAOONL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3187_length_18153_cov_15.778318"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLP50_11010"
     CDS             <1..662
                     /locus_tag="PLP50_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52120.1"
                     /translation="LVVLRNGEVRTFELAEVERIEFAGTSLTGVVDPARPVFRDPVGT
                     WDWVDGQTLVLRSDSQFALHRSDGTLISQGRWELLESATQRYRLVHARGGYIDTVALS
                     ADGWTLSGASNTGYRLEGRRRSDAATGWGGASEAQLAGTWDWADGRVLVLRTDRTFLA
                     YSGSRQTDEGRWQVLDAAARRIQLTHRLAGTTDTLVLSADGRMLEGTNNRGARVRGQK
                     R"
     gene            722..1444
                     /gene="rnc"
                     /locus_tag="PLP50_11015"
     CDS             722..1444
                     /gene="rnc"
                     /locus_tag="PLP50_11015"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPA52121.1"
                     /translation="MTRREEDLGRFEEALGFRFGDRALLERALTHSSYANEASRAGAV
                     RDNETLEFLGDSVLGFLVAEMLLERFPDFREGQLSKARSHLVSEGSFARIARGLGVGE
                     LLLLAAGESRSGGRVKESLLADAFEAVFAAITLDAGLPAAREVARRLFEGPVSELDPG
                     ELSFHDYKTALQELVQGEGKPLPVYRLVSESGPDHRKEFVFEVLFDEGLSARGTGGTK
                     KEAQRQAAKTALAALLGRATRA"
     gene            complement(1497..3284)
                     /locus_tag="PLP50_11020"
     CDS             complement(1497..3284)
                     /locus_tag="PLP50_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPA52122.1"
                     /translation="MTPRLARRLAAALAALLLSLPAAAQIGAPPEVLSLRLAADAEKI
                     VAGGSFRVAVVATIAPGWHVNSNAPLEDYLIPTEAALAPAEGLKLGPPAYPAYREAKL
                     PFSDKPLALYDGETVIVFEGTAEAAAAPGPRTLRATLDYQPCNDAQCLAPNQSEATLE
                     IEVVPAGTPVAQANAALFPLAGARAPEAGGAAEAAPAAAPESGPFSGRSLPAILGLVF
                     LAGLALNLTPCVYPLIPITVGFFSRQSEGKTSRTFGLAVAYVLGMSVTYSALGVFAAL
                     SGSLFGSWLQKPAVLVVIAAVVLALALSMFGLYELQAPHFITDRTGSKGGVLGALTMG
                     LFVGFVAAPCIGPFVLSLLTYVAQQGSAPLGFALFFTLAMGLGLPYLVLGTVSGSLKA
                     MPRSGEWMIAVRKVFGFVLVALAAWFLRPLLPERAFAWAVALPLLAGGVWFLFFEKAG
                     AGVRWFRGLKVALGLALLAVGAWFVVPSKHAEGLAFQPYSDAALASASAAGKPVVIDF
                     FADWCLPCKELEKFTFTDPAVAKALDGWVLLKADLTKSASPEVAALRTRWNVQGVPTI
                     VFVGPDGKETLPRVVQFEKPAAFLSRFPK"
     gene            complement(3296..4177)
                     /locus_tag="PLP50_11025"
     CDS             complement(3296..4177)
                     /locus_tag="PLP50_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52123.1"
                     /translation="MRKLRLTFLASAVLLTPLLFAQGPQTAFPSEGTDAPLDWATTMP
                     DALKRAGALAGGRLLVEIRDAQCPECERMEKLLYPSASFRGFLKDKVPVRLLRGTPDA
                     DQLFARYRLRTTPAWLVLSTDLMLCGKQEGDTNQSTWIERFFETEKAWAAFLQSIDEE
                     KANPADPARAFAVGEEAYRRYGDVMAERRFRKVSEDEQAPSELRAKSLAYLATFALEA
                     RRFDDAAKTLEKVLATSTDPTLLERAELRLADVEIGRGNREKAAARLNAFVEKHPQSP
                     LKAQAEELLRLLATARP"
     gene            4299..4374
                     /locus_tag="PLP50_11030"
     tRNA            4299..4374
                     /locus_tag="PLP50_11030"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4332..4334,aa:Phe,seq:gaa)
     gene            4494..5006
                     /locus_tag="PLP50_11035"
     CDS             4494..5006
                     /locus_tag="PLP50_11035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPA52124.1"
                     /translation="MAILKVMKLGADVLRRPAAEIEAKDLKRGRFKGLFDDLRETMVE
                     YAGTGLAAPQVAAPVRALLYMVDPEGRRASAAAVPPTILVNPSFEVLDPKEVEDWEGC
                     LSVPFLAARVPRARKVRVRALSETGMPLDFVAEDFHARVLQHEADHLDGVIYLDRVKD
                     RASIRYTVDF"
     gene            5012..5233
                     /locus_tag="PLP50_11040"
     CDS             5012..5233
                     /locus_tag="PLP50_11040"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPA52125.1"
                     /translation="MDDAPLSEKHARILRKYKPVCICNGIKFPRVEEAIESGARSLED
                     VARATGCTTGSCHGERCVPVILEMLDGKR"
     gene            5287..5982
                     /gene="nuoB"
                     /locus_tag="PLP50_11045"
     CDS             5287..5982
                     /gene="nuoB"
                     /locus_tag="PLP50_11045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPA52126.1"
                     /translation="MSDKKFPLPAARIEPPLAPGVPSTTGFDFLTTKLDWFVQWSHKN
                     SLWPMPFGTACCAIEFMSSVSSHYDLARFGAEVVRFSPRQADLMIVSGTITDKQAPIL
                     RKIYDQMPEPKWVISMGVCACTGGFYRAYHVVQGIDEIVPVDVYVAGCPPTPENLIYG
                     IIELQKRIQSGDLARHREAKTYVPIRVGTDPVESLPAYSKGVMAHGRSSESGFAAMEG
                     PVEAAKAGKGETR"
     gene            5979..7718
                     /locus_tag="PLP50_11050"
     CDS             5979..7718
                     /locus_tag="PLP50_11050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPA52127.1"
                     /translation="MSATLAPSAGVTFAPVLQRAFPDGAVRVEPRPAHDVPTLVATAE
                     AFPAAARFLRDDPSTSFDLLLDLVAVDRSRLDGPPSCRGEERFQLNALLYSTKRNEHL
                     RLRVALPATDPRLPTLVETWPASNWFEREAWDLMGIRFDGHPNLARLLTHNGFVGHAL
                     RKDYEAGQRWLCTDEDVLQTRLAAKTDLPEDVFETTTLNFGPSHPATHGTLRIVVRLD
                     GERVVAAESECGYLHRCFEKMSETHTYTQVIPYTDRLNYCSPMINGAGYARAVEQMLG
                     IEIPPRARAVRVVLSEFSRIMDHMVCIGANLVDVGGLTTFWYSYQAREEIYSLLEACC
                     GARLTSSYARIGGLSMDVPANFVEWCRSLSRSVRDRIDEVDTLLTKNRILHKRMMGTG
                     VVTKEQAIAWGFTGPCLRASGVGYDVRKDKPYDGYDEVDFDVPVATAGDNFSRFHVRM
                     EEMRQSLRIIEQVVSRLPGGPVVSDDYRVVLPPKEMVYNEMEGLIFHFKNVFEGIQVP
                     AGEVYSFTEGANGELGYSIVSDGSGRPYRVKVRPPCFPLFAAFEKIVVGGLVSDAVAT
                     LGTLNIIAGELDR"
     gene            7729..8403
                     /locus_tag="PLP50_11055"
     CDS             7729..8403
                     /locus_tag="PLP50_11055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPA52128.1"
                     /translation="MPGKVVTLEPPAETLAVRLYLPLLSGMALTFRKFVSNVLSRKAR
                     PTIEYPEERRAYSDRFRGRHILKTREDGTLKCVACYMCATHCPADCIHIEAGEHPNLA
                     YEKYPTVFEIDLLRCVMCGFCVDACPKDAIWMTKDYELSFFARKDAVYGIPELTEKPS
                     DTAPDGPGYGYRPYYGESPKRKAGQFEPSGLAVLPMTLARNADINRMPPPPPPKPPAP
                     APPKPA"
     gene            complement(8486..8905)
                     /locus_tag="PLP50_11060"
     CDS             complement(8486..8905)
                     /locus_tag="PLP50_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52129.1"
                     /translation="MLVPRGRALLLVASLLAGTAVAEPAVKARAGDEPGLRIHLPADL
                     LRPAPAAPELGVFSEESLIRLPGDPDSVLTLGGPVPSGEPRLRFAIEVNEAVLKLIPP
                     DPRVRADDTRSRAGGDWQPVDATGRPYQLRLGARLVW"
     gene            complement(9004..9801)
                     /locus_tag="PLP50_11065"
     CDS             complement(9004..9801)
                     /locus_tag="PLP50_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA52130.1"
                     /translation="MSPTTPPALSPLLDLGGRVALVTGASRGIGAATARLLGRCNARV
                     AIHFGRSRAEAEAVAAEIALSGGPPAALFQAELASDDERRRLHREVAAVLGDPLLLVH
                     NAGIYVRNPFEETDDAAFLSRWRTTQAVNLEAAAHLTLLSLPAMRAARFGRVVMVASR
                     AAFRAETDAASYAVSKAGMVSLARCLARNEGKHGITANAICPGWVDTAMARPDVAARR
                     AEIEGEVPLGRIATPEDCARAILFYLSPLASYANGTALGLNGGSFLH"
     gene            complement(9835..10695)
                     /locus_tag="PLP50_11070"
     CDS             complement(9835..10695)
                     /locus_tag="PLP50_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52131.1"
                     /translation="MSLWDAGVDLGATLAKAVAVPSGRPLEAFESFVAPAGDERLLDA
                     FLLRHSLRHLAATGGGASALADRLAPERAVAIVDEFAAWGAGEKLLTRRAGLELHSPH
                     LLVSLGTGTSILRMGARGSVARVGGTALGGGTLRGLGRLLLGTDDHERLVALAATGDR
                     RRVDLLVGDLYRPGGIALMPDLTAANFGKAREPRAADVAHALLGLVGENVALLAGQIA
                     VALATPPGGGAPRRRPDVLYAGSTLRNNPLLVEILGNVTALVGARASFLPFGEFTGAL
                     GALAKARAGA"
     gene            10849..14523
                     /locus_tag="PLP50_11075"
     CDS             10849..14523
                     /locus_tag="PLP50_11075"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HPA52132.1"
                     /translation="MHDSVLDALSDSFGDNAAFALELYAQYRLNPATVGDDWRRTFER
                     YEKAARSVAASGPGEAAEAAPAAPAPAAPAPAASASGRPAPALRTGETLHPLVGGAAT
                     IARNMDASLSVPTATSQRVIPVKAMEENRRILNLHREAVRQSKISFTHLVAWAVVRAL
                     EKHPGMNDAYAEAEGKPQRVRKPHVNLGIAVDVAKKDGSRSLLVPNIKLAEGLDFAEF
                     VATFDNLVGKARRGAIEPEAFLGTSISLTNPGTLGTTSSAPRLMPGQGCIIATGAMGY
                     PPEYLAMPDEVVAGLGVSRIMAVTSTYDHRIVQGAESGAFLATLQDLLVGGDEFYERI
                     FRDLKVPHRPVRWEPDRNPPFLGRGNRLEAVEKQARILPLINFYRVRGHLLADLDPLG
                     VDTPRYHAELDPATFGYTIWDLDRKFITNGLAGRDQATLREILEVLRQTYCGKIGAEF
                     MNIQDPAQKAWLMERMESCRNRASLSAEEKKRVLAKLVEAESFEKFLHAKYVGHKRFS
                     LEGGEAAIPLLTRLLDRAAEMGVEEAVIGMPHRGRLTILTTVVGKSVDRIFAEFEDVL
                     DPQSIQGSGDVKYHLGAVGVHEAPGGKRVKLGLAPNPSHLEAVDPVVEGMVRAKQERA
                     GDDAREKVLPILLHGDAAFAGQGLVAETLNMCQLEGYRTGGTIHVVVNNQIGFTTNPK
                     DARSSPYCTDVAKMVQAPIFHVNGDDPDAVVQAVDLAMEYRKTFGHDVVVDMVCYRRY
                     GHNEGDEPSYTQPLLYAKIRNHSSVARLYGDVLVREGVLSTAQVEEVWVDAKQALDKA
                     YDASAATRTRGAFSDSLVAPAAPRYPVAEGVRDRLLEVVRAVSTLPPGFEVHPKLRPL
                     LKKRADYGSGRPEIDWAGGEMLAFGLLLQEGTPVRLSGQDSGRGTFSHRHSVLADPRT
                     GAEWIPLNAIREPQARFEVIDSFLSEAGVMGFEFGYAVADPSTLVLWEAQFGDFANGA
                     QVIIDQFISGSEQKWSQTCDLVLLLPHGQEGQGPEHSSARLERFLTLCAEDNMRVANV
                     TTPAQYHHLLLRQMRDDVRRPLIVMSPKSLLRHPKAVSRFDEIADGAFRPVLDDTAFE
                     AGGADGVRRVVIASGKLVYELLAAREKASRSDVAIVRLEELYPFPGVELGRVIARYPK
                     DAALVFAQEEPKNMGAWRFVMEQFLDGAVPTAGGRPLRYAGRRPSASPAPGSHHVFAM
                     EQEAIAADALGVETAVPARS"
     gene            complement(14611..15243)
                     /locus_tag="PLP50_11080"
     CDS             complement(14611..15243)
                     /locus_tag="PLP50_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52133.1"
                     /translation="MIRRKLPLLPGLALIVLVFAAAGCASTATVVPGKTLALVEVTYP
                     EMRMSGVVLDTGEATPMSRMADSLLHQLGAEKAYEVKDARGRAHFSSLATDPAAMAAF
                     RQAVPADGYLGVQLLGCSAMPRTETERRGSGPGAVEVTVYFYRGECTAELTALDPEGK
                     KIAVLQKTGRYDSPRQDRQDSSQMGSQALTIAIENTARSLAREIRPTAGK"
     gene            complement(15303..16343)
                     /locus_tag="PLP50_11085"
     CDS             complement(15303..16343)
                     /locus_tag="PLP50_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPA52134.1"
                     /translation="MSATAPAAPKTNRIGLAKADYEGSKSTLCLGCGHDVITKQIVTA
                     FFELGIEPRRVAKFSGIGCSSKTPAYFLNRAWGFNAAHGRMPSVATGALLANPELVGI
                     GVSGDGDTASIGIGQFVHLVRRNIPLVYVVENNGVYGLTKGQFSATADVGSTLKGGGA
                     NELVPVDLCGLAIELGCGFVARSFSGDMKQLQAILQAAIAHRGTAVLDVVSPCVTFND
                     HEGSTKSYKYSKDHEEPLHEIGFVPYFDDTLAEIPPGETKDVPFPDGSLVRFRSVGRD
                     YDPTDKDQVLATLHRSRAAKEFLTGILYLEPEKPTFAKVLRTVEEPLTTMGADRLRPS
                     REALAAILEEYR"
     gene            complement(16340..18151)
                     /locus_tag="PLP50_11090"
     CDS             complement(16340..18151)
                     /locus_tag="PLP50_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPA52135.1"
                     /translation="MTVNDFSLQVATANGSGSQTANNVLLRSLFRMGVPVSGKNLFPS
                     NIQGLPTWFTIRASRHGYIGRKRELDVLVLMNPETAAADAIAAAPGTSVVYERKLAVE
                     KLRDDLRLFPVPFGELASKLPLPPEQAKLRRLLVNMIYVGVLSDLMGIEPVEIEASLR
                     KQLGGKAKAIELNLAALRAGLDYSAATFPEKLPHRVERMDATAGKLIIDGNSASALGC
                     LFGGATVCTWYPITPSSSLCETFIELCDRHRTDPGTGHTAAAIVQAEDEIASIGMVLG
                     AGWAGARAFTATSGPGISLMSELIGYGYYAEIPAVIFDVQRVGPSTGLPTRTMQGDVL
                     LCHTNSHGDGRHPVLFPASPEECFTMAQEAFDLAERLQTPVFVLTDLDLGMNNWMADP
                     FPFSDAPFDRGKVLDADGVERLRETWGRYRDLDGDGVPWRTLPGTDHPRAAFFTRGSG
                     HDERAAYTEKPDDYVRNMERLARKLETGRALLPKPVTEERPGAKAGLLAFGTSHYGTA
                     EGRDRLEREHGLPLDYLRLRALPIGDEARAFLERHEIVYVVEQNRDAQLAGLLRDALP
                     SCASRLVSVLQYDGLPLDATTVVDGVLAHARPAGGAR"
BASE COUNT         2689 a         6185 c         6761 g         2518 t
ORIGIN      
        1 tcctcgtcgt cctcaggaac ggagaggttc gaaccttcga gctggccgag gtggaacgga
       61 tcgagttcgc cggcacgtcg ctgaccggcg tcgtcgaccc ggctcgcccg gtcttccgcg
      121 accccgtagg cacgtgggac tgggtcgacg gacagacgct cgtcctccgc tccgactccc
      181 agttcgccct ccacaggagc gacgggacgc tgatcagcca ggggcgctgg gagctcctcg
      241 agagcgccac gcagcgctac aggctcgtcc acgcgcgcgg cgggtatatc gacaccgtcg
      301 cgctttccgc cgacggctgg acgctctccg gggcttcgaa cacgggctac cgcctcgagg
      361 gacggcgtcg ctccgacgcg gcgacgggct ggggcggcgc ctccgaggcg cagctcgccg
      421 ggacgtggga ctgggcggac ggccgcgtcc tggtgctgcg gaccgaccgg acgtttctgg
      481 cctactcggg gagccgccag acggacgagg gccgctggca ggtgctcgac gccgccgccc
      541 gccgcatcca gctgacgcac cggctggccg ggaccaccga cacgctggtc ctgtcggcgg
      601 acgggcggat gctcgaaggg acgaacaacc gcggcgcccg cgtccggggg cagaagaggt
      661 agaagagaac gcgccgggtc gagggtggcg acgcccgcgg cccggcggcc ggagagccga
      721 gatgacgaga cgagaagagg atctcgggcg gttcgaggag gcgctcgggt tccggttcgg
      781 ggaccgagcg ctcctcgagc gcgcgctgac gcactcctcg tacgcgaacg aggcttcgcg
      841 cgccggcgcc gtgcgcgaca acgagacgct cgagttcctg ggcgacagcg tcctcgggtt
      901 cctcgtcgcc gagatgctcc tggagcgctt ccccgacttc cgcgagggcc agctctcgaa
      961 ggcccggtcg cacctcgtgt cggaggggag cttcgcgcgg atcgcgcgcg ggctcggcgt
     1021 gggcgagctg ctcctcctcg cggcgggaga gagccggtcg gggggccgcg tgaaggagtc
     1081 gctcctcgcc gacgcgttcg aggcggtctt cgcggcgatc acgctcgacg ccggccttcc
     1141 ggcggcgcgc gaggtggcgc ggcggctctt cgaggggccg gtctccgagc tcgatccggg
     1201 cgagctgtcg ttccacgact acaagacggc gctccaggag ctcgtgcagg gggaggggaa
     1261 gccgcttccc gtctaccggc tcgtctccga gtccggcccc gaccaccgga aggagttcgt
     1321 cttcgaggtg ctcttcgacg aggggctctc ggcgagaggg accggcggga cgaagaagga
     1381 agcccagcga caggcggcga agaccgcgct cgcggcgctc ctcggccgcg cgacgcgcgc
     1441 ctgagcggcg gggcgcgcgg gagagggccg tccggtcccc tcccgcgcgc gggagctcac
     1501 ttcgggaagc gcgagaggaa cgccgccggc ttctcgaact ggacgacgcg cgggagcgtc
     1561 tccttcccgt ccgggccgac gaagacgatc gtcgggacgc cctgcacgtt ccagcgcgtg
     1621 cggagggcgg ccacctcggg ggaggcgctc ttcgtcaggt cggccttcag gaggacccag
     1681 ccgtcgaggg ccttcgcgac ggccggatcg gtgaacgtga acttctcgag ctccttgcag
     1741 gggaggcacc agtcggcgaa gaagtcgatg acgaccggct tcccggcggc cgaggccgag
     1801 gcgagcgccg cgtcggaata gggctggaag gcgaggccct ccgcgtgctt cgacgggacg
     1861 acgaaccagg cgccgacggc caggagcgcg aggccgagcg cgaccttcag gccgcggaac
     1921 cagcggacgc cggcgcccgc cttctcgaag aagaggaacc agacgccgcc ggcgaggagc
     1981 gggagggcga cggcccaggc gaacgcgcgc tcggggagga gcgggcggag gaaccaggcg
     2041 gcgagggcga cgaggacgaa gccgaagacc ttcctcacgg cgatcatcca ctcgccggag
     2101 cggggcatcg ccttgaggct ccccgagacc gtgccgagga cgaggtaggg gaggccgagg
     2161 cccatggcca gcgtgaagaa gagtgcgaag ccgagcgggg cggagccctg ctgggcgacg
     2221 taggtgagga gcgagaggac gaacggcccg atgcagggcg ccgccacgaa gccgacgaag
     2281 aggcccatcg tgagcgcgcc gaggacgccc cccttgctcc ccgtccggtc ggtgatgaag
     2341 tggggcgcct ggagctcgta gaggccgaac atcgagagcg cgagcgcgag gacgacggcg
     2401 gcgatgacga cgaggacggc cggcttctgg agccaagagc cgaagagcga tcccgagagc
     2461 gcggcgaaga cgccgagcgc cgagtaggtg acgctcatgc cgaggacgta ggcgaccgcg
     2521 aggccgaacg tgcggctcgt cttcccctcg ctctgccgcg agaagaagcc gacggtgatc
     2581 gggatgaggg ggtagacgca gggagtgagg ttgagggcga ggcccgcgag gaagacgagg
     2641 ccgaggatcg cggggaggga gcggccggag aacggaccgg actcgggcgc ggcggcgggg
     2701 gccgcctccg ccgcgcctcc cgcctcgggc gcccgggcgc cggcgagcgg gaagagcgcg
     2761 gcgttcgcct gggcgacggg cgtgccggcc gggacgacct cgatctcgag cgtcgcctcg
     2821 gactggttcg gcgcgaggca ctgcgcgtcg ttgcacggct ggtagtcgag cgtggcccgg
     2881 agcgtccgcg gtcccggtgc ggcagccgct tcggccgttc cctcgaagac gatgacggtc
     2941 tcgccgtcgt agagggcgag cggcttgtcg gagaagggga gcttcgcttc gcggtaggcg
     3001 gggtaggcgg gcgggccgag cttcagccct tcggccggcg cgagggcggc ctctgtcggg
     3061 atgaggtagt cctcgagggg cgcgttcgag ttgacgtgcc atcccggcgc gatcgtggcg
     3121 acgacggcga cacggaacga gcccccggcg acgatcttct cggcgtcggc cgcgaggcgg
     3181 agggagagga cctctggtgg ggcgccgatc tgggccgcgg cggggaggga gaggaggagg
     3241 gctgcgagcg ccgctgcgag gcggcgggcg aggcgtggcg tcattcgggt tcccttcacg
     3301 gtcgcgcggt ggccaggagg cggaggagct cctcggcctg ggccttcagc ggggactggg
     3361 ggtgcttctc gacgaaggcg ttcagtcgcg cggccgcctt ctcgcggttt ccccgtccga
     3421 tctccacgtc cgcgaggcga agctcggccc gctcgaggag cgtcgggtcg gtcgaggtgg
     3481 cgaggacctt ctcgagggtc ttcgcggcgt cgtcgaagcg gcgggcctcg agcgcgaacg
     3541 tggcgaggta ggcgagcgac ttcgcgcgga gctcggaggg ggcctgctcg tcctcggaga
     3601 ccttccggaa gcgccgttcg gccatcacgt cgccgtagcg ccggtaggcc tcctcaccga
     3661 cggcgaaggc gcgagccggg tcggccgggt tcgccttctc ttcgtcgatc gactggagga
     3721 acgccgccca cgccttctcg gtctcgaaga agcgttcgat ccaggtcgac tggttcgtgt
     3781 cgccttcctg cttcccgcag agcatcaggt cggtcgagag gacgagccag gccggcgtcg
     3841 tccgcaggcg gtagcgcgcg aagagctggt cggcgtcggg cgtcccgcgg aggagacgga
     3901 ccgggacctt gtccttcagg aagccccgaa aggacgcgga cgggtagagg agcttttcca
     3961 tccgctcgca ctccggacac tgcgcgtcgc ggatctcgac gagcaggcgc cctccggcga
     4021 gggcgcctgc tcgcttgagg gcgtcgggca tcgtcgtcgc ccagtcgagg ggcgcgtccg
     4081 tcccttcgga cgggaaggcc gtctgcggcc cctgggcgaa gagcagcggc gtcaggagga
     4141 cggcggaggc gaggaaggtt aggcgcagtt ttctcatttc gtctcggtcg tgggggtggc
     4201 ggcgcgaagg gccgcgttga ctattctagt gggcgccggt ataacgcccg gcgtcgcgcc
     4261 gaggggcgga tcgcaaggtg cgtgccgcgg cgccgggtgc ccaggtagct cagctggtag
     4321 agcacgcgcc tgaaaagtgc ggtgtcgacg gttcaactcc gcccctgggc accatctctt
     4381 ctctctcgcg gcccggagct tccgggccgt ttccgtttcg gggtgccgcg cccggttccg
     4441 ggcgggacgg ccaggccccg gttcgcgcct agaatccgtc tcgggagggc tgcttggcga
     4501 ttctcaaggt gatgaagctc ggggccgacg tgctgcgccg ccccgccgcg gagatcgagg
     4561 cgaaggacct gaagcgcggt cgcttcaagg gcctgttcga cgacctgcgc gagacgatgg
     4621 tggagtacgc ggggaccggg ctcgccgcgc cgcaggtcgc cgcgccggtg cgcgccctcc
     4681 tctacatggt cgacccggaa gggcgccggg cgagcgccgc cgcggtgccg cccacgatcc
     4741 tcgtcaaccc gtcgttcgag gtgctcgacc cgaaggaggt ggaggactgg gaagggtgcc
     4801 tgtcggtgcc gttcctcgcg gcacgggtcc cgcgcgcccg gaaggtgcgg gtccgggcgc
     4861 tctcggagac ggggatgccg ctcgacttcg tggcggagga cttccacgcc cgggtcctcc
     4921 agcacgaggc cgaccacctc gacggcgtga tctacctgga ccgggtgaag gaccgcgcgt
     4981 cgatccgcta cacggtcgac ttctgaggcc cgtggacgac gcccccctct ccgagaagca
     5041 cgcgcggatc ctccggaagt acaagccggt ctgcatctgc aacgggatca agttcccgcg
     5101 cgtcgaagaa gcgatcgaga gcggggcgcg aagcctggag gacgtcgcgc gggccacggg
     5161 ctgcacgacc ggctcctgtc acggcgagcg ctgcgtcccg gtcatcctcg agatgctcga
     5221 cggaaagcgc tgagagcccc ttacaatcgc gctcgtgatc ggttccacga gcaccgggag
     5281 tcctcgatga gcgacaagaa gttccccctg ccggccgcac ggatcgagcc gcccctcgcg
     5341 cccggcgtcc cctccacgac gggcttcgac ttcctgacga cgaagctcga ctggttcgtc
     5401 cagtggagcc acaagaactc cctctggccg atgccgttcg gcacggcctg ctgcgccatc
     5461 gagttcatgt cgtcggtctc gtcgcactac gacctcgccc ggttcggagc cgaggtcgtc
     5521 cgcttctcgc cgaggcaggc ggacctgatg atcgtctcgg ggacgatcac cgacaagcag
     5581 gccccgatcc tccggaagat ctacgaccag atgccggagc cgaagtgggt catctcgatg
     5641 ggcgtctgcg cgtgcaccgg gggcttctac cgcgcctacc acgtcgtcca gggaatcgac
     5701 gagatcgtcc ccgtcgacgt ctacgtcgcg ggctgccctc cgacgccgga gaacctgatc
     5761 tacgggatca tcgagctcca gaagcggatc cagtcgggcg acctggcccg tcatcgcgag
     5821 gcgaagacct acgtcccgat ccgcgtcgga accgaccccg tggagtcgct gccggcctac
     5881 tcgaagggcg tcatggcgca cgggcggagc tcggagtccg gtttcgccgc gatggagggt
     5941 cccgtcgagg cagcgaaggc cggaaaggga gagacgcgat gagcgcgacc ctcgcaccgt
     6001 ccgcgggcgt cacgttcgcc ccggtcctcc agcgggcgtt ccccgacggc gccgtgcgcg
     6061 tcgagccccg ccccgcccac gacgtcccga cgctcgtcgc gacggccgag gcgttcccgg
     6121 cggccgcccg gttccttcgc gacgacccat cgacctcctt cgacctcctc ctcgacctgg
     6181 tcgcggtcga ccggtcgcgt ctcgacggcc cgccgtcgtg ccgcggcgag gagcgtttcc
     6241 agctgaacgc gcttctctat tcgacgaagc ggaacgagca ccttcgcctc cgggtcgccc
     6301 tcccggcgac ggacccacgc cttccgacgc tcgtcgagac ctggccggcg tcgaactggt
     6361 tcgagcgcga ggcgtgggac ctgatgggga tccgtttcga cggccatccg aacctcgccc
     6421 gcctcctgac ccacaacggg ttcgtcgggc acgcgctccg gaaggactac gaggcgggac
     6481 agcgctggct ctgcaccgat gaggacgtcc tccagacccg cctcgccgcg aagacagacc
     6541 tccccgagga cgtcttcgag acgacgacgc tgaacttcgg cccgtcgcac cccgcgacgc
     6601 acgggacgct ccgcatcgtc gtgaggctgg acggcgagcg ggtcgtcgcg gccgagtcgg
     6661 agtgcgggta cctgcaccgc tgcttcgaga agatgtcgga gacgcacacg tacacccagg
     6721 tgatcccgta cacggaccgc ctgaactact gctctccgat gatcaacggc gccgggtacg
     6781 cccgcgccgt cgagcagatg ctcggcatcg agatcccgcc ccgcgcgagg gcggtccggg
     6841 tcgtcctctc ggagttctcg cggatcatgg accacatggt ctgcatcggc gcgaacctgg
     6901 tcgacgtcgg cggcctgacg acgttctggt actcgtacca ggcgcgcgag gagatctact
     6961 cgctcctcga ggcgtgctgc ggcgcgcggc tcacgtcgag ctacgcgcgg atcggcggtc
     7021 tctcgatgga cgtccccgcg aacttcgtcg agtggtgccg gagcctctcg aggtcggtcc
     7081 gggaccgcat cgacgaggtc gacacgctcc tgacgaagaa ccggatcctc cacaagcgga
     7141 tgatggggac cggcgtcgtc acgaaggagc aggcgatcgc ctggggcttc acggggccgt
     7201 gcctgcgcgc ctcgggcgtc ggctacgacg tccgcaagga caagccgtac gacggctacg
     7261 acgaggtcga tttcgacgtc cccgtcgcga cggcgggcga caacttctcc cgcttccacg
     7321 tccggatgga ggagatgcgg cagtcgctcc ggatcatcga gcaggtcgtg tccaggcttc
     7381 ccggcgggcc ggtcgtgagc gacgactacc gcgtcgtcct gccgccgaag gagatggtct
     7441 acaacgagat ggaagggctc atcttccatt tcaagaacgt cttcgagggg atccaggtcc
     7501 cggccgggga ggtctactcg ttcacggaag gcgcgaacgg cgagctcggc tactcgatcg
     7561 tctccgacgg gagcggccgt ccctaccgcg tgaaggtacg cccgccctgc ttccccctct
     7621 tcgcggcgtt cgagaagatc gtcgtcggcg gcctggtgtc cgacgcggtc gccacgctcg
     7681 ggacgctcaa catcatcgcc ggcgagctgg accggtagga gagacgcgat gcccggaaag
     7741 gtcgtcaccc tcgagccccc ggccgagacc ctcgccgtcc ggctctacct cccgctcctg
     7801 tcgggcatgg cgctcacgtt ccggaagttc gtctcgaacg tcctctcgcg gaaggcccgc
     7861 cccacgatcg agtaccccga ggagcgccgc gcctattccg accgtttccg cggacggcac
     7921 atcctgaaga cccgcgagga cgggacgctc aagtgcgtcg cctgctacat gtgcgcgacg
     7981 cactgcccgg ccgactgcat ccacatcgag gccggcgagc acccgaacct cgcctacgag
     8041 aagtacccga ccgtcttcga gatcgacctc ctccggtgcg tgatgtgcgg cttctgcgtc
     8101 gacgcctgcc caaaggacgc gatctggatg acgaaagact acgagctgtc gttcttcgcg
     8161 aggaaggacg ccgtctacgg catcccggag ctgacggaga agccgtccga cacggcgccg
     8221 gacgggccgg ggtacggcta ccggccctac tacggcgaga gcccgaagag gaaggccggc
     8281 cagttcgagc cctcgggcct cgccgtcctg ccgatgacgc tcgcgaggaa cgcggacatc
     8341 aaccggatgc cgcctcctcc gccgccgaag ccgccggcgc ccgcgccgcc gaagccggcg
     8401 tagccgaccg ccgacgcctc gaaacgtcga acgccccggg gtgctcccgg ggcgttctcg
     8461 ttcgcggcgt gtcgggccgc cggagctacc agacgaggcg ggcgccgagc cggagctggt
     8521 aggggcggcc ggtcgcgtcg accggctgcc agtcaccgcc ggcccggctc cgggtgtcgt
     8581 ccgcgcggac ccgcgggtcg ggcgggatca gcttcaggac cgcctcgttc acctcgatgg
     8641 cgaagcgaag ccgcggctcg ccggagggga cgggccctcc gagcgtcagg acggaatccg
     8701 ggtccccggg aaggcggatc aggctctcct cgctgaagac gccgagctcg ggggcggctg
     8761 gcgccgggcg gagcaggtca gcagggaggt gaatgcggag cccgggctcg tctcccgcgc
     8821 gggccttcac cgcgggctcg gcgacggccg taccggcgag gagggacgcg accagcaaaa
     8881 gggctctccc gcggggaacg agcacggccc gattctctcc caggaaggcg gccggctgca
     8941 cgcactttct cgcggccgcc ttccgagacg aaacggagcc ccgtcgaacg gccgcccgtc
     9001 gggtcagtgg aggaacgacc ccccgttcag cccgagcgcc gttccgttcg cgtaggaggc
     9061 gagcggggag agatagaaga ggatcgcccg ggcgcagtcc tccggcgtcg cgatcctccc
     9121 gagcgggacc tctccctcga tctcggccct gcgggcggcg acgtccgggc gcgccatcgc
     9181 cgtgtcgacc catccggggc agatcgcgtt cgccgtgatc ccgtgcttcc cctcgttccg
     9241 ggcgaggcag cgggcgagac tcaccatccc cgccttcgag acggcgtagc tggccgcgtc
     9301 ggtctcggcg cggaacgcgg cgcgggacgc gaccatcacc acccgtccga accgggcggc
     9361 ccgcatcgcg gggagcgaga ggagcgtgag gtgcgcggca gcctcgaggt tgacggcctg
     9421 ggtcgtcctc cagcgcgaga gaaaggccgc gtcgtcggtc tcctcgaacg ggttgcggac
     9481 gtagatgccg gcgttgtgga cgaggaggag cggatccccg aggacggccg ccacctcgcg
     9541 atggaggcgc cgccgctcgt cgtcgctcgc gagctcggcc tggaagagcg cggcgggagg
     9601 cccgccggag agcgcgatct ccgcggcgac ggcctcggcc tcggcgcggc tccggccgaa
     9661 gtggatcgcc acgcgagcgt tgcagcgccc caggaggcgc gccgtggcgg cgccgatccc
     9721 acgggacgcg ccggtcacga gggcgacccg tcctccgagg tcgaggagcg gggagagggc
     9781 ggggggcgtc gtcgggctca tcccgggagt ttcgcacccg gggtccccgg gacgtcaggc
     9841 cccggcgcgc gccttcgcga gggcgccgag cgcccccgtg aactcgccga acggcaggaa
     9901 cgaggcgcgc gcaccgacga gggccgtgac gtttcccagg atctcgacga ggagagggtt
     9961 gttccggaga gtcgatccgg cgtagagcac gtcgggccgc cggcggggcg ccccgccgcc
    10021 gggaggtgtc gcgagggcga cggcgatctg gccggcgagg agcgccacgt tctcgccgac
    10081 gagcccgagg agggcgtggg cgacgtcggc agcgcgcggc tcgcgcgcct tgccgaagtt
    10141 ggcggcggtc aggtcgggca tgagggcgat ccccccggga cgatagaggt cgccgacgag
    10201 gaggtcgacg cgccggcggt cgcccgtggc cgcgagggcg acgagccgct cgtggtcgtc
    10261 ggtgccgagg aggaggcggc cgaggccgcg gagcgttccg ccgccgaggg ccgtcccgcc
    10321 gacgcgggcc acgctccccc gggcgcccat ccggaggatc gaggtgccgg tcccgaggct
    10381 gacgaggagg tggggcgagt ggagctcgag accggcgcgg cgcgtgagga gcttctctcc
    10441 ggcgccccac gcggcgaact cgtcgacgat cgccaccgcc ctctccggcg cgagccgatc
    10501 ggcgagcgcc gacgcgccgc ccccggttgc cgcgaggtga cggagggaat gccgcaggag
    10561 aaaggcgtcg aggaggcgct cgtcgccggc cggcgcgacg aacgactcga aggcctcgag
    10621 cggccggccc gaggggaccg ccacggcctt cgcgagggtc gcgccgaggt cgacgccggc
    10681 gtcccagagg ctcatcgcgg gggattgtcc cccgtaaagg gccgcgacgg agcgggcgcc
    10741 ggcgcccgcg ggcgtcgggg taacatgacc gcgctgccgg ccgcccggcc gacacggggc
    10801 gatgacgccc gccgggcgca gacgacccgg ccgacaggga gaccctgaat gcacgactcc
    10861 gtcctcgacg ctctctccga ctcgttcggc gacaacgccg cgttcgcgct cgagctctac
    10921 gcccagtacc ggctgaaccc ggccacggtc ggcgacgatt ggcggaggac cttcgagcgg
    10981 tacgagaagg ccgcgagatc ggtcgcggcg tcggggcccg gggaggcggc ggaggccgcg
    11041 cccgcggcgc cggcgcccgc ggcgccggcc ccagccgcct ccgcgtccgg ccgccccgcg
    11101 ccggcgctcc gcaccggcga gacgctgcat ccgctcgtcg gcggcgccgc gacgatcgcg
    11161 cggaacatgg acgcctcgct ctcggtcccg acggcgacgt cccagcgcgt gatcccggtc
    11221 aaggcgatgg aggagaaccg ccgcatcctg aacctccatc gcgaggcggt ccggcagtcg
    11281 aagatctcct tcacgcacct cgtggcgtgg gccgtcgtcc gggcgctcga gaagcacccc
    11341 gggatgaacg acgcgtacgc cgaagcggag gggaagcccc agcgcgttcg caagccgcac
    11401 gtgaacctcg gcatcgccgt cgacgtcgcg aagaaggacg gaagccgatc cctcctcgtc
    11461 ccgaacatca agctcgccga agggctcgac ttcgccgagt tcgtcgcgac gttcgacaac
    11521 ctcgtcggga aagctcgccg cggcgccatc gagccggaag cttttctcgg gacgtcgatc
    11581 tcgctgacga accccgggac gctcggaacg acgtcgtccg ctccgcgcct gatgcccggc
    11641 cagggatgca tcatcgcgac cggcgcgatg ggctatccgc cggagtacct cgcgatgccc
    11701 gacgaggtcg tcgcggggct cggcgtctcc cggatcatgg cggtgacgtc gacctacgac
    11761 caccggatcg tccagggggc ggagtcgggc gcgttcctcg cgacgctcca ggatctcctc
    11821 gtcggcggcg acgagttcta cgagcggatc ttccgcgacc tgaaggtccc gcaccggccg
    11881 gtccgctggg agccggaccg caacccgccg ttcctcggcc gcgggaaccg cctcgaggcg
    11941 gtcgagaaac aggcccgcat ccttccgctc atcaacttct accgggtccg cggccacctc
    12001 ctggccgacc tcgacccgct cggcgtcgac acgccccggt accacgccga gctcgacccg
    12061 gcgacgttcg gctacacgat ctgggacctc gaccggaagt tcatcacgaa cggcctcgcg
    12121 ggacgcgacc aggcgacgct ccgcgagatc ctggaggtcc ttcgccagac ctactgcgga
    12181 aagatcggcg ccgagttcat gaacatccag gaccccgccc agaaggcgtg gctcatggag
    12241 aggatggagt cgtgccggaa ccgggcgtcg ctctccgccg aggagaagaa gagggtcctc
    12301 gcaaagctcg tggaggccga gagcttcgag aagttcctcc acgccaagta cgtcgggcac
    12361 aagcgcttct ctctcgaggg gggagaggcg gcgatcccgc tcctgaccag gctcctcgac
    12421 cgcgcggcgg agatgggcgt cgaggaggcc gtcatcggga tgccgcaccg cgggcgcctc
    12481 acgatcctca cgacggtcgt cgggaagtcc gtcgaccgga tcttcgcgga gttcgaggac
    12541 gtcctcgacc cgcagtcgat ccagggctcg ggcgacgtga agtaccacct cggcgccgtc
    12601 ggcgtccacg aggcccccgg cgggaagcgg gtgaagctcg gtctcgctcc gaacccgagc
    12661 cacctcgagg cggtcgaccc ggtcgtcgag ggaatggttc gcgccaagca ggagcgcgcg
    12721 ggcgacgacg cgcgcgagaa ggtcctgccg atcctcctgc acggcgacgc ggccttcgcg
    12781 gggcaggggc tcgtcgccga gacgctgaac atgtgccagc tcgagggcta ccggacgggc
    12841 ggaacgatcc acgtcgtcgt gaacaaccag atcggcttca cgacgaaccc gaaggacgcc
    12901 cgctcgtcgc cgtactgcac ggacgtcgcc aagatggtcc aggccccgat cttccacgtg
    12961 aacggggacg acccggacgc ggtcgtccag gccgtcgacc tcgcgatgga gtaccggaag
    13021 acgttcgggc acgacgtcgt ggtcgacatg gtctgctacc gccgctacgg ccacaacgag
    13081 ggggacgagc cgagctacac gcagccgctc ctctacgcga agatccggaa ccactcctcc
    13141 gtggcccgtc tctacggcga cgtcctcgtc cgcgaggggg tcctgtcgac ggcccaggtg
    13201 gaagaggtct gggtcgacgc gaagcaggcg ctcgacaagg cctacgacgc ctccgcggcg
    13261 acgcgcaccc gaggggcctt ctccgactcc ctcgtggcgc ccgccgcgcc ccgctatccc
    13321 gtcgcggaag gcgttcgcga ccgtctgctg gaggtcgtcc gggccgtctc gacgctcccg
    13381 ccgggcttcg aggtccaccc gaagctgagg ccgctcctga agaagcgggc cgactacggg
    13441 agcggccgtc ccgagatcga ctgggcgggg ggcgagatgc tcgcgttcgg gctgctcctc
    13501 caggaaggga cgccggtccg cctctcgggg caggactccg gacgcgggac gttcagccac
    13561 cgccactcgg tcctggcgga cccgaggacg ggcgccgagt ggatcccgct gaacgcgatc
    13621 cgcgagccgc aggcgcgctt cgaggtcatc gactccttcc tctcggaggc cggcgtcatg
    13681 gggttcgagt tcggctacgc cgtggccgac ccgtccaccc tcgtcctctg ggaggcgcag
    13741 ttcggcgact tcgcgaacgg cgcacaggtc atcatcgacc agttcatctc cggctccgag
    13801 cagaagtgga gccagacgtg cgacctcgtc ctcctcctgc cgcacggtca ggaggggcaa
    13861 gggcccgagc actcgtcggc gcgcctcgaa cgcttcctga cgctctgcgc cgaggacaac
    13921 atgcgggtcg cgaacgtgac gacgcccgcc cagtaccacc acctgctgct gcggcagatg
    13981 cgggacgacg tgcggcggcc tctcatcgtc atgtccccga agagcctcct gcgtcacccg
    14041 aaggccgtct cccggttcga cgagatcgcg gacggcgcgt tccgcccggt cctcgacgac
    14101 acggcgttcg aggcgggcgg agcggacggc gtccgacgcg tcgtgatcgc gagcggcaag
    14161 ctcgtctacg agctgctcgc cgcgcgcgag aaggcgtcgc gctccgacgt cgcgatcgtg
    14221 cggctcgagg agctctaccc gttcccggga gtggagctgg gccgcgtgat agcgcgctac
    14281 ccgaaggacg ccgctctggt cttcgcgcag gaagagccga agaacatggg agcgtggcga
    14341 ttcgtcatgg agcaattcct cgacggcgcc gtgccgacgg caggaggccg acctctccgc
    14401 tacgcgggcc gacggccctc ggccagcccg gcaccgggct cgcaccacgt cttcgcgatg
    14461 gagcaggagg cgatcgcggc cgacgcgctc ggcgtcgaga cggcagtccc ggcccgctcc
    14521 tgaggcgacg gacggttcgc gcgtcgggaa gtggcggcgc ggggcggccc gggaagggtc
    14581 gtccgcgcgc cgcctcgggc gcccgagacg ctacttcccg gccgtcggac ggatctcgcg
    14641 agcgaggctg cgcgcggtgt tctcgatggc gatggtcagg gcctgcgagc ccatctgcga
    14701 ggagtcctga cggtcctgcc tcgggctgtc ataccggccg gtcttctgga ggacggcgat
    14761 cttcttgccc tcggggtcga gcgcggtcag ctcggcggtg cactccccgc ggtagaagta
    14821 gaccgtcacc tcgaccgcgc ccggtccgga gccgcggcgt tcggtctcgg tcctcggcat
    14881 ggcgctgcat ccgagcagct ggacgccgag gtagccgtcg gcggggacgg cctgccggaa
    14941 ggccgccatc gccgccgggt cggtcgccag cgaggagaag tgggcccgtc cgcgcgcgtc
    15001 cttcacctcg taggccttct cggccccgag ctggtggagg agcgagtccg ccatccggga
    15061 catcggggtc gcctctcccg tgtcgagaac gacgccgctc atgcgcatct cggggtaggt
    15121 gacctcgacg agcgcgaggg tcttcccggg gacgacggtc gcggtggagg cgcagcccgc
    15181 ggcggcgaag acgaggacga tgagggccaa gccgggaagg agaggcagct ttcggcgaat
    15241 catctgatca ttatgccgcg ccgggcggcg ggaggacagc cggtcgacag cgcgcgcgcg
    15301 cctcaccggt actcctccag gatcgcggcg agggcctcgc gcgacgggcg caggcgatcc
    15361 gcgcccatcg tcgtgagcgg ctcctcgacg gtcctcagca ccttcgcgaa ggtcggcttc
    15421 tcgggctcga ggtagaggat gccggtcagg aactccttcg cggcccggct ccggtggagc
    15481 gtcgcgagga cctggtcctt gtcggtcggg tcgtagtcgc ggccgaccga gcggaaccgg
    15541 acgaggctcc cgtcggggaa cggcacgtcc ttcgtctcgc cgggcgggat ctccgcgagg
    15601 gtgtcgtcga agtacgggac gaagccgatc tcgtggagcg gctcctcgtg gtccttcgag
    15661 tacttgtagc tcttcgtcga cccctcgtgg tcgttgaacg tgacgcaggg ggagacgacg
    15721 tcgaggaccg ccgtgccgcg gtgggcgatc gcggcctgga ggatcgcctg gagctgcttc
    15781 atgtctccgg agaaggagcg ggcgacgaat ccgcagccga gctcgatcgc gaggccgcag
    15841 aggtcgaccg ggacgagctc gttcgcgccg ccccccttca gcgtcgagcc gacgtcggcg
    15901 gtggcggaga actgcccctt cgtcaggccg tagacgccgt tgttctcgac gacgtagacg
    15961 agcgggatgt tccgcctcac gaggtggacg aactgcccga tgccgatcga ggcggtgtcc
    16021 ccgtcgccgg agacgccgat gccgacgagc tccgggttcg cgaggagggc gcccgtggcg
    16081 acggagggca tccggccgtg ggcggcgttg aagccccagg cgcggttcag gaagtaggcc
    16141 ggggtcttcg aggagcagcc gatccccgag aacttcgcga cgcgccgggg ctcgatcccg
    16201 agctcgaaga aggcggtgac gatctgcttc gtgatgacgt cgtggccgca gccgaggcag
    16261 agggtcgact tcgaaccttc gtagtccgcc ttcgcgaggc cgatccggtt cgtcttcggg
    16321 gcggcgggag cggtcgcgct catcgggcac ctcctgcggg gcgggcgtgg gcgaggaccc
    16381 cgtcgacgac cgtcgtcgcg tcgagcggga gcccgtcgta ctggaggacg gagacgaggc
    16441 gggaggcgca ggaggggagc gcgtcgcgga ggaggcccgc gagctgggcg tcgcggttct
    16501 gctcgacgac gtagacgatc tcgtgcctct cgaggaaggc ccgggcctcg tcgccgatcg
    16561 ggagcgcccg gagcctcagg tagtcgagcg gcaggccgtg ctcccgctcg agccggtcgc
    16621 gcccctcggc cgtcccgtag tgggacgttc cgaacgcgag gaggcctgcc ttcgcaccgg
    16681 ggcgctcctc cgtgacgggc ttcggcagga gcgcgcggcc ggtctcgagc ttgcgcgcga
    16741 ggcgctccat gttcctcacg tagtcgtccg gcttctcggt gtaggcggcc cgctcgtcgt
    16801 ggccggagcc gcgtgtgaag aacgcggcgc gcgggtggtc ggtgccgggg agcgtccgcc
    16861 acgggacccc gtcgccatcg aggtcgcggt agcggcccca ggtctccctc aggcgctcga
    16921 ccccgtcggc gtcgaggacc ttgccccggt cgaagggggc atcggagaag gggaacgggt
    16981 cggccatcca gttgttcatc ccgaggtcga ggtccgtcag gacgaagacg ggcgtctgga
    17041 gcctctcggc gaggtcgaac gcctcctgag ccatcgtgaa gcattcctcg ggcgaggccg
    17101 ggaagaggac ggggtgccgg ccgtcgccgt gggagttcgt gtggcagagg aggacgtctc
    17161 cctgcatcgt ccgggtcggg aggcccgtcg aggggccgac gcgctggacg tcgaagatca
    17221 ccgccgggat ctcggcgtag tagccgtagc cgatcagctc cgacatgagc gagattccgg
    17281 ggcccgaggt cgccgtgaag gcgcgcgctc ccgcccagcc ggcgccgagg accatgccga
    17341 tcgaggcgat ttcgtcctcg gcctggacga tcgcggccgc ggtgtgtccc gtgcccgggt
    17401 cggtccggtg gcggtcgcag agctcgatga acgtctcgca gagcgaggag gatggcgtga
    17461 tcgggtacca ggtgcagacg gtggcgccgc cgaagaggca gccgagggcc gacgcggagt
    17521 tgccgtcgat gatcagcttg cccgcggtcg cgtccatccg ctcgacgcgg tgggggagct
    17581 tctcggggaa cgtcgccgcc gagtagtcga gacccgcgcg gagggccgcg aggttcagct
    17641 cgatcgcctt cgcctttccg ccgagctgct tccggaggga ggcctcgatc tcgaccggct
    17701 cgatccccat caggtcggag aggacgccga cgtagatcat gttcacgaga aggcgacgga
    17761 gcttcgcctg ctcgggcggg agcgggagct tcgaggcgag ctctccgaac ggcaccggga
    17821 agaggcggag gtcgtcgcgg agcttctcga cggcgagctt ccgctcgtag acgacggatg
    17881 tcccgggggc ggcggcgatg gcgtcggccg cggcggtctc cgggttcatc aggacgagga
    17941 cgtcgagctc gcgcttcctc ccgatgtagc cgtggcggct cgcccgaatc gtgaaccagg
    18001 tcgggagacc ctggatgttc gacgggaaga ggttctttcc cgagacgggg acccccatcc
    18061 ggaagaggga ccggagaagg acgttgttcg cggtctgaga gccggagccg ttcgccgtgg
    18121 cgacctggag agagaagtcg ttgacggtca cgg
//
LOCUS       DAOONL010000070        17744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3299_length_17744_cov_22.237266, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000070 DAOONL010000000
VERSION     DAOONL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17744
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3299_length_17744_cov_22.237266"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLP50_11095"
     CDS             <1..429
                     /locus_tag="PLP50_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPA52136.1"
                     /translation="HDVGKMALPDSVLLKPGELTEEEQAVMRRHPEHAWEMLKGVDFL
                     RPALDIPYAHHERWDGTGYPRGLSGAEIPLAARLFAVVDVWDALCYDRAYRAGWPARR
                     VQEHLREGAGTQFDPSVVPAFLRILERDGEPVFADPASGR"
     gene            complement(474..1202)
                     /locus_tag="PLP50_11100"
     CDS             complement(474..1202)
                     /locus_tag="PLP50_11100"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA52137.1"
                     /translation="MARPRPAPGKRPREARGRSTAAGPAAAADPARISPFRLPRRLAP
                     RKRPAQARARETVEAILEAAVELFSSRGYARTSTNHLAARAGVSVGSLYQYFPNKDAI
                     LTALLARHLEAVHDAVAASLPVLADPAVPVRAGVRGLLERLRAVHEADPRLARAVEQQ
                     AGQMPSVPAGLAAHRHGTVERIAGILGSRPDVRSGNHALMAALLGEVTEAVSGFLVHG
                     PREAFGREELLDEAVEAVARYVER"
     gene            1257..1862
                     /locus_tag="PLP50_11105"
     CDS             1257..1862
                     /locus_tag="PLP50_11105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA52138.1"
                     /translation="MIPTESPIRTPPPARSGHVCPAWAGPLLCCPLRRLLGSPEKLLS
                     PHVSPGMAVLEPGPGMGWFTLPLARLVSPAGRVVARDLQPAMVAGLRRRAKKAGLLSR
                     IDAAACAADDLSLVEWQGRIDAAFLIHVLHEVPDHRRLLTQVHAALRAGGRLLLLEPK
                     GHVSAESLAGEVAEAARVGFRVVSEVAERRSHGALLEKPGA"
     gene            complement(1904..3610)
                     /locus_tag="PLP50_11110"
     CDS             complement(1904..3610)
                     /locus_tag="PLP50_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA52139.1"
                     /translation="MVIESGAHEPLRVLLVEDSADDAEIVRRLLSRDGLSPLMERVQN
                     ADEMSAALEASGWDVVLADDHLPSFSSAAALSLVRARGTDVPFLIVSGAIGRDAAVRA
                     LRAGADDVVSKSDLARLVPALRRGLQEAENRRARRTAEEQLTRIRTHDPLTGLVNGDE
                     LLRRLTTAVSTIDRTGRPVSFVLLDLDGFRSLNGALGRAAGDAILQGVAVALQGVVSE
                     GETVARIGVDEFGVLLPGLDAEEATRRGARLVETVSALRLRSAGRALVVRASAGVVTI
                     TSGDRAADDVLREATAACVAAKELGGRRVRRFDRSDPAVMKRWTEAEWVPRLLDALDN
                     DRFVLYAQSLLPLSPAAPRESVTEVLVRLVDADDRLVLPGEFVPAAERFGLAAAIDRW
                     VVRNLLHRVSSHPPRSEENGPGTLLVNLSGATLSDLAFAEDVEGMLRRSACDPSRFCF
                     EVTETAVVRDLEAAVDLMARLSRLGCRFALDDFGTGLSSLAYLRSLPVQLLKIDGAFI
                     RDLARDATARAIVESVVHLARALDLGTVAEWVEDSATLERVRTAGLDYAQGFAIHTPE
                     PF"
     gene            complement(3638..4174)
                     /locus_tag="PLP50_11115"
     CDS             complement(3638..4174)
                     /locus_tag="PLP50_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52140.1"
                     /translation="MRDRSVISSNVDVAAAISRSLAARRPGAPTGDEPRELYVRFERV
                     VPAEASSPRPPAAEIGTWEAFLAWSLELTRAHAGFVVDSQGFVIASRGNTPKDGFEGI
                     GAELCYAMDQLRRINPTGGELRLLELQFDANRLIGLRADAGAMGTILVGFQGTRSIGD
                     DTRDTLLRQLGQVLPGLA"
     gene            complement(4146..4958)
                     /locus_tag="PLP50_11120"
     CDS             complement(4146..4958)
                     /locus_tag="PLP50_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPA52141.1"
                     /translation="MTHVISLVSSKGGCGKTTTALNLGVAFAERGRRVVVVDLDPQGA
                     VGLALDRPDGEWPGLAEHVIEGRPVAGLVVETRLPGFCLLPRGRLDPVDTCSYEAFLH
                     SSGKVREVVRELSAGRDYVLLDTPSGVGAISRAALAASDWALLPLQAEPLALRSIGQA
                     LRVIEHVSREENRDLRLLGILPTMVQVRDEASFNVLRTAWARLGGVLESVVPRAPVFL
                     RASEAGLPVSFLAGRVTPEARRFEQIVIELETLIAESAGGGEVDERPQRDLL"
     gene            complement(4955..5824)
                     /locus_tag="PLP50_11125"
     CDS             complement(4955..5824)
                     /locus_tag="PLP50_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52142.1"
                     /translation="MSVTQAHEAIALASFLVETAERRGSGWLEARQGGAPVGTVVFRD
                     GQVAWASSAGQKEHLGHFLERIGYLSPEQLQETHARLRAARPDRKLGRLLEDAGLISR
                     PVLRICLLFHLRAAVSSLLLSESLAGDWEEGLFCSEDELTFPVVDVLPEWLLDRTPGI
                     GTPPEDLASTLLPLAEVPGYRGALAADWMGRLVACHGFGNGERGCAGQVAAAALAILG
                     GPCALGRSRQGFLDADEGVVVARWLDRDRRLLIALRLGADGRPGTALHRLGLVSPDIL
                     RSLDPQNEGALIP"
     gene            complement(5829..6128)
                     /locus_tag="PLP50_11130"
     CDS             complement(5829..6128)
                     /locus_tag="PLP50_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52143.1"
                     /translation="MIAIRVPPGCLRSAPVREYELSEPVDEALMHRLAEGGTLQYFPT
                     FPRPLFRIDRPQEFCTQGIVGSPVLRVTFSPSSDAGTREALRLALGGGTAEGEEG"
     gene            complement(6140..7276)
                     /locus_tag="PLP50_11135"
     CDS             complement(6140..7276)
                     /locus_tag="PLP50_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPA52144.1"
                     /translation="MVYLNHAATTHPRPPEVVSAVVAALSSAPVHPGRGAGGQDPLTE
                     ARLALAELLDVDGAERIALLPSATYALNLAIAGLAPQPGDHVVTTRLEHNSVLRPLAH
                     AAAAGRLRVSYVSPDRLGRVSTADVERAIEPRTRLVAVTHVGNATGCIQPIREIAAVA
                     AQKGVALLVDASQSAGVLPLGHRSLPGRVFVAFAGHKSLLGPPGVGGLVLADDRLPQG
                     VVGGTGIRSEEPLHPPELPLRHEAGTPDVPAAAGLAAGVRLVLARGVAEEGARRGRLA
                     IRLRSRLAATAGVSLSPELEPDPRAGIVAFRLAGWTPGDVGDALLRSFDIEVRAGLHC
                     APLVHDPLGTAPLGTVRASIGWSTTEADVDALAGAVDALAGGGA"
     gene            complement(7311..7892)
                     /locus_tag="PLP50_11140"
     CDS             complement(7311..7892)
                     /locus_tag="PLP50_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52145.1"
                     /translation="MTVMRDQVVKSLVQVEVKANEDGLMPGFNVLVNQLPTSFWNTVA
                     ERFMAIAPPDRKADMEKALVNCAYECGYHTGYGIVSSPEFQSVVMPMVTEGAKDVLRG
                     AFAVFTAWGWAKSGVVQVRDGERMVIRAVDYYEADSGGSGLRAYMVRGVSAAFMELAY
                     AEPYPNGMNTFSCEQTLGREVGDPHGEFVITKK"
     gene            complement(7906..8421)
                     /locus_tag="PLP50_11145"
     CDS             complement(7906..8421)
                     /locus_tag="PLP50_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52146.1"
                     /translation="MNLELSHTFDEASFRHFVNGHNVVLHCHHYMSLTSRLSEDFADL
                     GGPRVLREATEDSIRPVLDSYFAEHKVTAPADRLDIGRQYFSAMGLGRMEVSGSATEG
                     QVTLRHSHVDEGWVQKWGAHDKPVNHVGCGFVAAMFAAAFDRPARSFQVTESASIVMG
                     APASVLAVRQA"
     gene            complement(8432..9208)
                     /locus_tag="PLP50_11150"
     CDS             complement(8432..9208)
                     /locus_tag="PLP50_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPA52147.1"
                     /translation="MTQRAGRKSAIPPAEVEGLKGNLAMTSIPDLLQFLNASGKTGQL
                     LLEKHGDRPEGRTYFSAGALVHATAGPAIGMEALVEMCGWDRGSFAFYDNVLSPKASI
                     SMPVQQALMEAIRVLDERRREEARPARQDSERSHRMAKSTRTSTDVLEDFLKVPGVLS
                     AVIVGRDGFVIESAGGSSTVNIEDLGAALANAINGVEEMGSELQVSKFQDLFVEYGRA
                     VIMCRPAGDAIIVVVAPDASKLGIIRHKVKPLVDELSQIL"
     gene            complement(9366..10703)
                     /locus_tag="PLP50_11155"
     CDS             complement(9366..10703)
                     /locus_tag="PLP50_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA52148.1"
                     /translation="MIDDDARLCETLVDALGDETTLVLHAQTAAAGLALCRRVTADVV
                     LLDQNLPDATGAAICPALVEQNETTKVVFMTAFPTFENAVAALKVGASDYIAKPFELE
                     EVRLAVRNAFRVRELERIELLHSRRQREELERSAFVGSAPSLGMPLRLADVAAASDVP
                     VLVTGETGTGKSLLARSIHYRSHRRDHPFVAQNCAAFPEHLAESELFGHEKGAFTGAV
                     APRRGLFEMAVDGTVFLDEIGAMALPLQAKLLGVIEDRTVRRIGSEVVRRAHARVVAA
                     TNSDLEEAVANGAFRQDLFYRLSVIRIHLPPLRERIEDLPALCRHLLRTCTGKDLELS
                     RQEIDRLAEYPWPGNVRELANVLERARLVQPEDRLEPSKLLSVRTASPPARPAAGPEA
                     ETATLEEVERRHVSATIERCGGNLTHAAAVLGIALSTLRRKVGEYGLRADRRN"
     gene            complement(10767..12473)
                     /locus_tag="PLP50_11160"
     CDS             complement(10767..12473)
                     /locus_tag="PLP50_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA52149.1"
                     /translation="MDAGRGPDELVGTLVAGRYRLDARIGSGALGIVYRARHVLIGRE
                     LAIKVLAFDRPGSDVYRPWFLREARAANRVNHPNVAEIYDYGETDDGLVYLVMELLVG
                     ERLSDRIAAGAMGTRQSLEIVEQVASALGRAHQLGVVHRDVKPDHVFLLRRGDQADFV
                     KLIDFGLAHVAWERRLGPPGAVLGTPEYIAPEQARGEEPGPAADLYSLGVVLFEMLTG
                     RLPFLLEDAADLAEAHRSQPPPDPRLLRPGLDEGAAALTLRLLEKDPARRFRDAPHLA
                     EACRGVLVRLQSDGSPVLRGGDPQPAPAPPSFAEGSLWAVAASLLGRMAAAANPSGGC
                     PEAVSRSVEEIWARVAELARVDGELQLLETCSENLRLRGRETAERIGLQIEELARQGS
                     RIAREEEELADVIRRLEEERAAAVRERDAAREAAEEGDVRGVLAESHRAREEAAAAAA
                     RSERSDEAIRRAREELRRREGALRQIEARSERLRDQLERETSRLEADREAGAPRLLRL
                     ATDREVLVRRLGVVARGLREHFSRRSECRDLVCRLDELELLSDGDGRPPRPMPARGSG
                     PV"
     gene            complement(12476..14350)
                     /locus_tag="PLP50_11165"
     CDS             complement(12476..14350)
                     /locus_tag="PLP50_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52150.1"
                     /translation="MTKSAPRSRLRVLLVEDSADDALIVLRALEKGESEFDAIRVASR
                     EELVTALADGPVDVVLTDVVLPSFSGLAALQLVAERAPDTPVIVVSGAVGEEQAVALM
                     RAGASDFVDKRNIRRLPVVVERELHAARLRGAERRAREALSASEARRRSLLESLPVGV
                     FRLSPDGHVVSANPCLLEMLGYGTEDDLAAVPCPALWIDSATVRRAAERNGIAESEIE
                     TSRRDGSVFWAFLRLRATLREDGTLAHLDGTLEDSTEKRELERAILIGKTEWERTFDA
                     VRELIVLTDPFLRIARLNRALAERLGAEPATLVGRHWQEVLGEPLPPVDLARLVEASE
                     HRGSIEYDYASSSLGGEFRFSTTPRLDDRGVIIGAIHVGRDVTTQRQIEELLRRQVAV
                     DQAEAIFRTFRHEVGNAMNTLKATLTVLRESHERFDTEHRERYIVRCLETVRIAENLL
                     GSLRRYQTLDRVDFERVRVGELLKERSDLLLATARERGIDCRLEVGDGPSLVSGDPDA
                     MTRVLLNAIDNAATALAGRPDALLELTCRRVGGNVVVEVRDNGSGIARDHLPHVFSPL
                     FTTKSDGSGMGLAIVQKLMLRMNGLALIRSEPGAGTTLELRFPRIDGGGSWPPVAAVS
                     "
     gene            14437..16341
                     /locus_tag="PLP50_11170"
     CDS             14437..16341
                     /locus_tag="PLP50_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPA52151.1"
                     /translation="MALRVAPVVPEGDAPTAAPAPELDRTEARLFVEALLLAVTDAVW
                     VCDASGRLVLANDAFGAALGRPAGALRGLTPEEVFSPDLAARVRRAQNRVMGTGAPLR
                     VQEFLYPVGSVERRLFETSFGSIRGPGGKPWGTVAVTRDVTHDRLGDEELLGTRDFLF
                     ALVNAVGPGWTSNRDHRLTLVSRAAAEIFGHPPSIVIGKTPEQLLPEEEVGTIVALQE
                     EAFRTGREVSRELELRGPEGRAASFLVRAFPYVGPASEALVVSLCTDVTEIRRLGRGL
                     AAAGDFLSGILDAIPDPVFAKDREHRWVFVNDAMCALVGRDRDQLLSHLDSEFFGASQ
                     VPGIHERDDEVFRTGRESLVEEDLTVRTGETKRVVVRKSFHVDPEGRPLVVGVIRDVT
                     KTRSDEAALRRANRRLEAANSELEAFVFAASHDLVAPLRRIESFSEFLLEGCLDRLEL
                     DGQELLRRIWSTARSGEQLTEDLLTLSRATRGELLRERIDLSSLARELADGLLEDSPG
                     RAVEIDVADGVVVDADRRLLRLCLENLLGNALKFTRGREARIEVGEEGRDVEGERVYF
                     VRDNGVGFEMAFADRLFGVFQRLHRAEDYPGSGIGLALVQRIVHRHGGRVWAESAVGQ
                     GTTIRFTLGV"
     gene            complement(16609..>17744)
                     /locus_tag="PLP50_11175"
     CDS             complement(16609..>17744)
                     /locus_tag="PLP50_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52152.1"
                     /translation="SDEWFSSLAADPRIGVDFGGVMPSAELAAQQAFFEMKAKTSALK
                     GLAATLGGMDGRKVLVLVSHRFSRYAGLEFLLKDRAGAEALGDPRTRQFDSKRLLEEV
                     ARAANANGVTLHGVFPADLDAAMPSADRSRLSAPGLSDQPLGGKEQIVLDNELEALSF
                     VAEKTGGVAAVGAGNVDRFVDRVASDLESWYSIGYPAPPGAGKAVPVTVEVKGRKLDV
                     RVRSSLVEKTTAEQMADRVLAHLFQPDGRARIPISATASPIPAQDGKFRIRVEVRIPI
                     ASLVLLPTAKGVEGAFSVLVASVAAAGDFSEVTRRSQAFEIPAADLETAKAGHYTYEL
                     EIVATGPDARVCVGIWDEKGSDAGFAVVTSAAPPASPATPPAG"
BASE COUNT         2548 a         6250 c         6517 g         2429 t
ORIGIN      
        1 cacgacgtcg ggaagatggc gcttcccgac tccgtcctcc tgaagccggg cgagctgacc
       61 gaggaggagc aggcggtcat gcggcgtcac ccggagcacg cctgggagat gctgaagggg
      121 gtcgacttcc tccgccccgc gctcgacatc ccctacgcgc accacgagcg atgggacggc
      181 acgggctacc cgcgcggcct ctcgggcgcc gagatcccgc tcgccgcgcg cctcttcgcg
      241 gtcgtcgacg tctgggacgc cctgtgctac gaccgcgcct accgggcggg ctggccggca
      301 cggagggtcc aggagcacct ccgcgaagga gccgggacgc agttcgaccc gtcggtcgtc
      361 cccgcgttcc tgcggatcct cgagcgcgac ggcgagcccg tcttcgccga cccggcgagc
      421 ggacggtaga aggcggagag gcgtcggcgt ggcccggaac ggcccgccgc gcctcagcgc
      481 tccacgtacc gggcgaccgc ctcgaccgcc tcgtcgagga gctcctcgcg tccgaaggct
      541 tcgcgcggac cgtggacgag aaatccgctc acggcctcgg tcacctcgcc gaggagcgcc
      601 gccatcaggg cgtggttccc gctcctgacg tcggggcggc tcccgaggat cccggcgatc
      661 cgctcgacgg tgccatgtcg gtgcgcggcg agcccggccg ggaccgacgg catctgcccg
      721 gcctgctgct cgacggcgcg cgcgagccgc gggtcggcct cgtggaccgc gcggaggcgc
      781 tcgaggagcc cgcggacgcc ggcgcggacc gggacggccg gatcggcgag gaccgggagc
      841 gaggcggcga cggcgtcgtg aaccgcctcg aggtggcgcg cgaggagggc ggtcaggatc
      901 gcgtccttgt tcgggaagta ctggtagagg gagccgaccg agaccccggc ccgcgcggcc
      961 aggtggttcg tgctcgtccg cgcgtacccc cttgaggaga agagctcgac ggcggcctcc
     1021 aggatcgcct ccacggtctc ccgggcgcgg gcctgcgccg ggcgcttgcg cggcgcgagg
     1081 cggcggggga ggcggaacgg cgaaatgcga gccgggtcgg cggcggcggc cggaccggcg
     1141 gcggtggagc ggccgcgggc ctcgcggggc cgcttcccgg gtgcgggacg gggacgagcc
     1201 atcgatgcct ccaaaagcga gtcgacgatg cgagtaatcg ctcgtatctt ctgggcatga
     1261 taccgaccga gagcccgatc cgaaccccgc ctcccgcccg cagcggacac gtctgcccgg
     1321 cctgggcggg gccgctcctc tgctgcccgc tccggcgtct tctcggctct cccgagaagc
     1381 tcctctcgcc gcacgtctcg ccggggatgg cggtcctcga gccgggaccc gggatgggct
     1441 ggttcacgct gccgctcgcg cggctcgtct ctcccgcggg gcgcgtcgtg gcgcgcgacc
     1501 tccagcccgc gatggtcgcg gggctgagga ggcgtgcgaa gaaggcgggg ctcctctcgc
     1561 ggatcgacgc cgcggcctgc gccgcggacg acctctcgct cgtggagtgg caggggcgta
     1621 tcgacgcggc gttcctgatc cacgtcctcc acgaggtgcc cgaccatcgg cgcctcctca
     1681 cgcaggtcca cgccgcgctc cgcgccggag ggaggctcct cctgctggag ccgaaggggc
     1741 acgtctccgc agagtcgctc gcgggcgagg tcgccgaggc cgcgcgcgtc gggttccgcg
     1801 tggtctcgga ggtggcggag cgccggagcc acggcgcgct cctcgagaag cccggggctt
     1861 gaacggaggc ggaggtccgg gcgcggcgtg aaggccggcg gcttcagaag ggttccggag
     1921 tgtggatcgc gaagccctgg gcgtagtcga gtccggccgt tctgacgcgc tcgagcgtgg
     1981 cggagtcctc gacccactcg gcgaccgttc cgaggtcgag cgctcgcgcc aggtgaacga
     2041 ccgactcgac gatcgctctc gcggtcgcat cgcgggcgag gtcgcggatg aacgcgccgt
     2101 cgatcttcag cagctggacg gggagcgacc gcaggtaggc gagggacgac agcccggtcc
     2161 cgaagtcgtc gagcgcgaat cgacagccga ggcggctcag ccgggccatc aggtcgaccg
     2221 ccgcctccag gtcccggacg acggcggtct ccgtgacctc gaaacagaat cgggaaggat
     2281 cgcaggccga tcgccgcaac atcccttcca cgtcctccgc gaaagcgagg tcgctgagcg
     2341 tggcaccgga gaggttcacg agaagcgtgc ccggaccgtt ctcctcggag cgtggcgggt
     2401 gtgacgacac gcggtggagc aggttccgga ccacccatcg atcgatggcc gcggccaggc
     2461 cgaaccgctc ggccgccggg acgaactctc ccgggaggac gagacggtcg tcggcgtcga
     2521 cgagacggac gaggacctcg gtcacgctct ctctcggcgc ggcggggctg agggggagga
     2581 ggctctgcgc gtagaggacg aagcgatcgt tgtcgagggc gtcgaggagg cgggggaccc
     2641 actccgcttc ggtccagcgc ttcatcaccg cggggtccga gcgatcgaag cgccggaccc
     2701 ggcggccgcc gagctccttc gccgcgacgc aggccgcggt ggcttccctc aggacgtcgt
     2761 ccgcggcgcg atcgccggag gtgatcgtga cgacgccggc gctcgctctc acgacgagcg
     2821 cgcgcccggc cgaacggagg cgcagcgccg agaccgtctc gacgagccgg gcgccgcggc
     2881 gagtcgcctc ctcggcgtcg agacccggga gcaggactcc gaactcgtcg accccgatac
     2941 gcgcgacggt ctcgccttcc gagaccaccc cttgcagggc caccgccacg ccctgcagga
     3001 tggcgtcccc cgcggcccgg ccgagggcgc cgttcagcga ccggaacccg tcgagatcga
     3061 gaaggacgaa ggagacgggc cgtcccgttc ggtcgatcgt cgagaccgcg gtcgtcagcc
     3121 gacgcaggag ctcgtcgccg ttcacgaggc cggtcagcgg atcgtgcgtg cggatccggg
     3181 tcagctgctc ctccgccgtc cggcgcgcac gccggttctc cgcctcctgg agcccgcgac
     3241 ggagggccgg cacgaggcgt gcgagatccg acttggagac gacgtcgtcg gcgccggccc
     3301 gaagcgcccg gaccgcggcg tcgcgtccaa tcgcacccga gacgatgagg aacggaacat
     3361 cggttccccg ggcccggacc aggctgagtg cggccgcgga gctgaacgac ggaaggtggt
     3421 cgtccgccag gacgacgtcc cagcccgacg cctccagcgc ggccgacatc tcgtcggcgt
     3481 tctggacgcg ctccatcagg ggcgacagtc cgtcgcggct caggagacgc cggacgatct
     3541 cggcgtcgtc cgccgagtcc tcgacgagca ggacgcgcag cggctcgtgg gcaccgctct
     3601 cgatcaccac gtcgccacgg gtctctcgtc gagagcgtca ggcgagcccg ggcagaacct
     3661 gcccgagctg ccgcagcagg gtgtcgcgcg tgtcgtcgcc gatcgagcgg gttccctgga
     3721 agccgacgag gatcgtcccc atcgcgccgg cgtccgcgcg gaggccgatg agccggttcg
     3781 cgtcgaactg gagctcgagc agccggagct cgccgccggt cgggttgatc cgtctgagct
     3841 ggtccatcgc gtagcagagc tcggcgccga ttccctcgaa gccgtccttc ggcgtgttgc
     3901 cgcggctggc gatgacgaac ccttgcgagt ccacgacgaa gccggcgtgc gcccgcgtca
     3961 gctcgaggct ccaggcgagg aacgcctccc aggtcccgat ctcggccgcc gggggacgcg
     4021 gactcgacgc ttccgccggg acgacgcgct cgaaccggac gtaaagctca cgaggctcgt
     4081 cgccggtcgg cgcgccgggc ctgcgcgccg cgagcgaacg gctgatggcg gcggcgacgt
     4141 cgacgttaga ggagatcacg ctgcggtctc tcatcgacct ctcctcctcc ggccgactcg
     4201 gcgatcaacg tttccagctc gatcacgatc tgctcgaagc gccgggcctc cggcgtgacg
     4261 cgccccgcca ggaaggagac gggaagaccc gcttcgctgg cgcgcaggaa gaccggcgcg
     4321 cgcgggacga cgctctccag cacgcctccg agccgcgccc acgcggtacg gaggacgttg
     4381 aaggacgcct cgtcccggac ctggaccatc gtcggcagga tgccgaggag gcgcaggtcg
     4441 cggttctcct cgcgggagac gtgctcgatc acgcggaggg cctggccgat cgagcggagc
     4501 gcgagcggct cggcctggag gggcaggagc gcccagtcgc tcgccgcgag cgcggcgcgg
     4561 gagatcgccc cgactccgga cggcgtgtcg aggaggacgt agtcccgccc cgcggagagc
     4621 tcacggacga cctcccggac cttcccggag gaatgcagga aggcctcgta gctgcaggtg
     4681 tcgaccgggt ccaggcggcc gcgtggaagg aggcagaagc ccgggaggcg cgtctccacg
     4741 accaggccgg cgacgggccg accttcgatg acgtgctccg ccaggccggg ccactcgccg
     4801 tccggacggt cgagggcgag gccgacggcg ccctgcggat cgagatcgac gacgacgacg
     4861 cggcgccctc gctcggcgaa ggccacgccg aggttcaacg cggtggtcgt cttgccgcac
     4921 ccgcccttgg agctcacgag ggagatgacg tgcgtcatgg gatcagggct ccttcattct
     4981 ggggatcgag cgatcgaagg atgtcggggg agacgagtcc gagacggtgg agggccgttc
     5041 ccgggcgccc gtcggctccg agcctcaacg cgatcaggag gcgtcggtcc cgatcgagcc
     5101 accgggcgac gacgacgccc tcgtccgcat cgaggaaccc ctgccggctc cgacccagcg
     5161 cgcaggggcc gccgaggatg gcgagcgcgg ccgccgcgac ctggccggcg cagcccctct
     5221 ccccgttccc gaatccgtgg cacgcgacga ggcgccccat ccagtcggca gcgagggcgc
     5281 cgcggtagcc gggaacctcg gcaagaggaa gcagcgtcga tgcgaggtcc tccggcggtg
     5341 tcccgatgcc cggggtccgg tcgagaagcc actccggcag gacgtcgacg acgggaaacg
     5401 tcagctcgtc ctccgagcag aagagccctt cctcccagtc gcccgcgagc gactcggaga
     5461 ggaggaggct ggagaccgcg gcgcggaggt gaaacaggag gcagatcctg aggacgggtc
     5521 gggagatgag gccggcgtcc tcgaggagac gtcccagctt ccggtcgggt ctcgccgcgc
     5581 ggagccgggc gtgtgtctcc tggagctgct cgggagagag gtagccgatc cgctccagga
     5641 agtgccccag gtgctccttc tggccggcgc tggaggccca cgcgacctgc ccgtcgcgga
     5701 agacgaccgt gcccacggga gcgccgccct gccgcgcctc gagccaaccg ctcccgcgtc
     5761 gctcggccgt ctccaccagg aaggaggcga gcgcgatcgc ctcgtgcgcc tgcgtcacgg
     5821 acaccggctc agccctcctc tccttcggcc gtccctccgc cgagcgcgag gcgcagcgcc
     5881 tcgcgggtgc cggcgtcgga ggaaggcgag aaggtcacgc ggaggaccgg tgaccccacg
     5941 atcccctgcg tgcagaactc ctgcgggcga tcgatccgga acagcggtcg cgggaaggtc
     6001 gggaagtact ggagcgtccc gccctcggcg aggcgatgca tcagcgcctc gtcgaccggc
     6061 tcggagagct cgtactcgcg gacgggcgcg ctccggaggc agccgggcgg cactcggatg
     6121 gcgatcacct cccgcaccgt caggcgcccc cgccggcaag cgcgtcgacg gcaccggcga
     6181 gcgcgtcgac gtcggcctcg gtggtggacc agccgatgct cgcccggacg gtcccgagag
     6241 gcgccgtgcc gagcggatcg tgaacgagcg gcgcgcagtg gaggcccgct cggacctcga
     6301 tgtcgaacga gcggaggagg gcgtccccga cgtcgcccgg cgtccacccg gcgagccgga
     6361 acgcgacgat gccggcgcga gggtccggct cgagctctgg cgagagggag acgccggcgg
     6421 tcgcggcgag acgcgaacgg agccgtatcg cgagccgccc ccggcgcgca ccttcctcgg
     6481 cgacgcctct cgccaggacg aggcgcacac cggcggcgag gcccgccgcc gcggggacgt
     6541 cgggcgttcc cgcctcgtgg cggagcggaa gctccggagg atggaggggt tcctcgctcc
     6601 gaatgccggt cccgcccacc acgccctgcg ggaggcggtc gtcggccagg acgaggccgc
     6661 cgacgccggg cgggccgagg agcgacttgt gccccgcgaa ggcgacgaag acccgcccgg
     6721 gcagcgatcg gtgaccgagc ggaaggacgc cggccgactg agaagcgtcg acgaggagag
     6781 cgacgccctt ctgcgccgcg accgcggcga tctcccggat cggctggata cacccggtcg
     6841 cgttgccgac gtgggtgacg gcgacgagcc gggtccgggg ctcgatcgcg cgctcgacgt
     6901 ccgccgtcga cacgcgaccc agccggtccg gcgagacgta cgagacgcgg agccgccccg
     6961 cggcggccgc gtgggcgagc ggtcgaagga cggagttgtg ctcgaggcga gtcgtcacga
     7021 cgtggtcgcc gggctgcggc gccagcccgg cgatcgcgag gttcagggcg taggtggcgg
     7081 acggaaggag agcgatccgc tccgcgccgt cgacgtcgag aagctcggcg agggcgaggc
     7141 gcgcctcggt gagggggtcc tgcccgccgg cgccgcgccc cgggtggacg ggcgccgacg
     7201 agagcgcggc gacgaccgcc gagaccacct cgggtggtct cggatgggtc gtggccgcgt
     7261 ggttcaggta gaccatctcg gtgggactcc ctccggccga gggctcggcg tcacttcttg
     7321 gtgatgacga actccccgtg cgggtcgccg acctcgcggc cgagcgtctg ctcgcaggag
     7381 aacgtgttca tcccgttcgg atagggctcc gcataggcca gctccatgaa ggcggcgctc
     7441 acgccgcgga ccatgtaggc ccggagcccg gagccgccgc tgtcggcctc gtagtagtcg
     7501 acggcgcgga tgaccatccg ctcgccgtcg cggacctgga ccactccgga cttcgcccag
     7561 ccccacgccg tgaacacggc gaacgctccg cgaaggacgt ccttcgcccc ttccgtcacc
     7621 atcggcatga cgacggactg gaactcgggg gagctgacga tcccatagcc ggtgtgatag
     7681 ccgcactcgt aggcgcagtt gacgagcgcc ttctccatgt cggccttccg gtcgggaggc
     7741 gcgatcgcca tgaagcgctc ggccacggtg ttccagaacg acgtggggag ctggttgacg
     7801 aggacgttga agccgggcat caggccgtct tcgttggcct tgacttcgac ttggacgagg
     7861 ctcttgacga cctggtcacg catgacggtc atgacgctcc tccgctcagg cctgccggac
     7921 ggccagcacg ctcgccgggg cgcccatcac gatcgaggcg ctctcggtca cctggaagct
     7981 ccgggccggg cggtcgaagg ccgccgcgaa catcgcggcg acgaatccgc acccgacgtg
     8041 gttgacgggt ttgtcgtgag caccccattt ctggacccat ccctcgtcga cgtgggagtg
     8101 cctgagcgtg acctggccct cggtcgcgct cccggagacc tccatccggc cgagccccat
     8161 cgcggagaag tactggcggc cgatgtcgag acggtcggcg ggagccgtca ccttgtgctc
     8221 cgcgaagtag ctgtcgagga cgggccggat cgagtcctcc gtcgcctcgc gcaggacccg
     8281 ggggcccccg aggtcggcga agtcctccga gagcctcgag gtgaggctca tgtagtgatg
     8341 gcagtggagg acgacgttgt gcccgttcac gaagtgacgg aacgaggcct cgtcgaaggt
     8401 atggctcagc tcgaggttca tctcgcccgc ctcacaggat ctgggacagc tcgtcgacga
     8461 ggggcttgac cttgtggcgg atgatcccga gcttcgaggc gtccggcgcg acgacgacga
     8521 tgatcgcgtc tccggcgggc cggcacatga tcaccgctcg tccgtactcg acgaacaggt
     8581 cctggaactt ggacacctgg agctcgctgc ccatctcctc gacgccgttg atggcgttgg
     8641 caagcgccgc gccgaggtcc tcgatgttca cggtgctgga tccgccggcg gactcgatca
     8701 cgaacccgtc gcggccgacg atgaccgcgg agaggacccc gggcaccttc aggaagtcct
     8761 cgaggacgtc ggttgaggtt cgggtggatt tcgccattct gtggctcctt tcgctgtcct
     8821 gtcgcgccgg ccgggcttcc tcccggcgac gctcgtcgag aactcggatc gcctccatca
     8881 gcgcctgctg gacgggcatg ctgatcgagg ccttcgggga gaggacgttg tcgtagaagg
     8941 cgaagctgcc ccggtcccag ccgcacatct cgacgagggc ctccatcccg atcgcgggac
     9001 cggccgtcgc gtggacgagc gcgccggcgg agaagtaggt tcgcccctcc ggccggtctc
     9061 cgtgcttctc gagcaggagc tgtccggtct tgccgctcgc gttcaggaac tggaggagat
     9121 cggggatgct cgtcatcgcg aggttcccct tcaggccctc cacctccgcc gggggtatcg
     9181 cgctcttccg gcccgccctc tgcgtcatga acgcctccac gatctggaca tgcccgcctc
     9241 cggacggtac tcagcgaagc gcgtgccagg ccgcggcgcg ggacgagacc gcgcagacag
     9301 ccgttccctc gcgcgtcaga ggtccgactc gctcgatttc ggccgctcga aacgggcggg
     9361 tcggctcagt tccggcggtc ggcccggaga ccgtactccc cgaccttccg gcgaagcgtc
     9421 gagagcgcga tgccgagaac cgcggcggcg tgcgtgaggt tcccgccgca acgctcgatc
     9481 gtggcggaga cgtgccgcct ctcgacctcc tcgagcgtcg ccgtctcggc ctcgggcccc
     9541 gccgccggcc gggcgggcgg cgacgccgtc cggacggaga ggagcttcga cggctcgagg
     9601 cgatcctcgg gctgaacgag gcgggcccgc tcgaggacgt tcgcgagctc gcgcacgttc
     9661 ccgggccagg ggtactcggc gagacggtcg atctcctgcc gggacagctc caggtccttc
     9721 cccgtgcacg tccgaaggag gtgccggcag agcgcgggca ggtcctcgat tcgctcccga
     9781 agagggggga ggtggatccg gatgacggac agccgataga agaggtcctg gcgaaacgcg
     9841 ccgttcgcca cggcttcctc gaggtccgag ttcgtcgccg cgacgacgcg ggcgtgcgca
     9901 cggcggacca cctcgctccc gatacggcgt accgtccggt cctcgatgac cccgaggagc
     9961 ttggcctgga gcggaagcgc catcgcgccg atctcgtcca ggaagaccgt gccgtccacg
    10021 gccatctcga agagcccgcg tcggggggcg accgctccgg tgaaggcccc cttctcgtgc
    10081 ccgaagagct ccgactcggc gaggtgctcg gggaaggcgg cgcagttctg ggcgacgaac
    10141 ggatgatcgc ggcgatggct ccggtagtga atggagcggg ccagcagcga cttccccgtc
    10201 ccggtctccc ccgtcacgag aacggggacg tcgctggccg cggcgacgtc ggcgagccgc
    10261 agcggcatcc cgagcgaggg cgccgacccg acgaacgccg agcgctccag ctcctcccgc
    10321 tgccggcggc tgtgcaggag ctcgatccgc tcgagctcgc ggacgcggaa ggcgttccgg
    10381 accgcgagcc ggacctcctc gagctcgaag ggcttcgcga tgtagtccga ggcgccgacc
    10441 ttgagggccg cgacggcgtt ctcgaaggtc gggaacgcgg tcatgaacac gaccttcgtc
    10501 gtctcgttct gctcgacgag cgccggacag atcgccgcgc ccgtcgcgtc cgggaggttc
    10561 tggtcgagga ggacgacgtc cgcggtcacg cgtcggcaga gagcgagccc ggcggcggcg
    10621 gtctgggcgt gcaggacgag cgtcgtctcg tccccgagcg cgtccacgag cgtctcgcag
    10681 agccgggcgt cgtcgtcgat cacgacgacc gtgaaccccg gactcggtgc ggccggctcc
    10741 gcgcgaaacc ccaaagcgcg gggatgttac acgggcccgg atccgcgcgc gggcatcggc
    10801 cgcgggggcc gcccgtctcc gtcggacagg agctcgagct cgtcgagccg gcagacgagg
    10861 tcccggcact ccgagcgccg cgagaagtgc tcccgaaggc cgcgcgccac gactccgagc
    10921 ctccggacga ggacctcgcg gtcggtcgcg agacgcagga gccgcggcgc gcccgcctcc
    10981 cggtccgcct ccaggcgcga cgtctcgcgc tcgagctgat ctctcaggcg ctccgagcga
    11041 gcctcgatct gccggagcgc gccctcgcga cgcctgagct cctcgcgggc ccgacgaatc
    11101 gcctcgtcgc tccgctcgct gcgggcggcg gccgcggcgg cctcctctcg cgcccgatgg
    11161 ctctccgcga ggacgccgcg aacgtcgccc tcctcggccg cttcgcgggc cgcgtcccgc
    11221 tcgcggacgg ccgcagccct ctcctcctcg aggcgacgga tcacgtccgc gagctcctcc
    11281 tcctcgcggg cgatccgcga gccctgccga gccagctcct cgatctggag gccgatcctt
    11341 tcggcggtct cccgccctct cagcctgagg ttctccgagc aggtctcgag cagctggagc
    11401 tccccgtcga cacgggcgag ctcggcgaca cgggcccaga tctcctccac gcttcgggag
    11461 accgcctccg ggcagcctcc ggacgggttc gcggccgccg ccatccgacc gaggagggac
    11521 gcggccacgg cccagagaga gccctcggcg aacgacgggg gagcgggcgc cggctggggg
    11581 tcgcctccgc gcaggacggg gctgccgtcg gactggaggc gaacgaggac cccccggcac
    11641 gcctccgcca ggtggggtgc gtcgcggaaa cgccgggcgg ggtccttctc gaggagccgc
    11701 agcgtgaggg ccgcggcgcc ctcgtcgagt ccgggcctga ggagacgcgg gtccggaggc
    11761 ggctgggaac ggtgggcctc ggcgaggtcc gcggcgtcct cgaggaggaa cggcaaccga
    11821 cccgtcagca tctcgaaaag gacgacgccg agggagtaga ggtcggccgc gggaccgggc
    11881 tcctcgcccc gggcctgttc cggcgcgatg tactccgggg tccccaggac ggcgccgggc
    11941 ggaccgagcc ggcgctccca cgcgacgtgc gcgagaccga agtcgatcag cttgacgaag
    12001 tccgcctggt cccctcggcg gaggaggaag acgtggtcgg gcttcacgtc cctgtggacg
    12061 acgccgagct ggtgcgcccg tccgagcgcg gaggccacct gctcgacgat ctcgaggctc
    12121 tggcgcgtgc ccatcgcgcc ggcggcgatc cggtcggaga gccgctcccc gacgaggagc
    12181 tccatcacga ggtagacgag gccatcgtcc gtctcgccgt agtcgtagat ctccgcgacg
    12241 ttcgggtggt tgacgcgatt cgcggcgcgc gcctcccgga ggaaccaggg ccgatagacg
    12301 tcgcttccgg gccgatcgaa ggcgaggacc ttgatcgcga gctcgcgtcc gatgagaacg
    12361 tgcctcgccc ggtagacgat gccgagcgcc ccggacccga tccgcgcgtc caggcggtat
    12421 cggccggcga cgagagtccc gacgagctcg tccggtccgc gcccggcgtc cacgctcaag
    12481 agaccgcggc cacgggaggc cacgacccgc cgccgtcgat acgcgggaac cgcagctcca
    12541 gcgtcgtgcc ggctccgggc tcgctccgga tgagagcgag cccgttcatc cggagcatga
    12601 gcttctggac gatcgcgagg cccatgcccg aaccgtccga cttcgtcgtg aagagcggag
    12661 agaagacgtg cgggaggtga tcccgggcga tgccggaccc gttgtcgcgg acctcgacga
    12721 cgacgttccc gccgacccgc cggcaggtga gctccaggag ggcgtcgggc cggcccgcga
    12781 gcgcggtggc ggcgttgtcg atcgcgttca ggagaacccg ggtcatcgcg tcggggtcac
    12841 cggagacgag gctcgggccg tcccccacct cgagccggca gtcgattccc ctctctcgcg
    12901 ccgtcgccag gagaaggtcg ctccgctcct tcaggagctc tccgacgcga acgcgctcga
    12961 agtcgacccg gtcgagagtc tgataccggc ggagcgagcc gaggaggttc tcggcgatcc
    13021 gaacggtctc gagacagcga acgatgtacc gctcgcgatg ctccgtgtcg aaccgctcgt
    13081 gactctcgcg aaggaccgtc agcgtcgcct tcagggtgtt catcgcgttc ccgacctcgt
    13141 gccggaacgt ccggaagatc gcttccgcct ggtccacggc gacctgtcgg cgaaggagct
    13201 cctcgatctg gcgctgggtc gtgacgtccc gccccacgtg gatggcgccg atgatgactc
    13261 cccggtcgtc gaggcggggc gtcgtgctga accggaactc gccgcccagc gagctgctcg
    13321 cgtagtcgta ctcgatcgaa ccgcgatgct cgctcgcctc gacgaggcga gcgaggtcca
    13381 ccggcggaag cggctcgccg agcacctcct gccagtgtcg gccgacgagg gtggccggct
    13441 cggcgccgag cctctcggcg agagctcgat tgaggcgtgc gatccggagg aacgggtcgg
    13501 tcagcacgat cagctcgcga acggcgtcga acgtccgctc ccactccgtc ttgccaatga
    13561 ggatcgccct ttcgagctcc cgcttctccg tcgagtcctc gagcgttccg tcgaggtggg
    13621 ccagagtgcc gtcctcccgc agggtcgctc gcagccgcag gaatgcccag aagacggaac
    13681 cgtcccggcg gctcgtttcg atctcgctct ccgcgatacc gttccgctcg gcggcccgac
    13741 ggacggtcgc ggagtcgatc cagagcgccg gacacggaac cgcggcgagg tcgtcctccg
    13801 tcccgtaccc gagcatctcg aggaggcacg ggttcgcgga gacgacgtgg ccgtcgggag
    13861 agagccggaa gacaccgacc ggaagcgact cgagaaggct cctccgtctc gcctccgacg
    13921 ccgagagagc ttcccgcgcg cggcgctccg ctccgcgcag ccgggcggcg tgaagctctc
    13981 tctccacgac gacgggcaga cggcggatgt ttcgcttgtc gacgaagtcg ctcgccccgg
    14041 cccgcatgag ggccacggcc tgctcctcgc ccaccgcgcc ggacacgacg atcacgggtg
    14101 tgtcgggcgc cctctccgcc acgagctgga gagccgcgag accgctgaag gacggaagga
    14161 ccacgtccgt caggacgacg tccacggggc cgtccgcgag cgccgtgacc agctcctccc
    14221 gggacgcgac ccggatcgcg tcgaactcgg attcgccttt ctcgagcgcc cggaggacga
    14281 tcagggcgtc gtcggcggag tcttcgacga ggagcacacg gaggcgcgac cgcggcgccg
    14341 acttcgtcac gtcggagatt ctaggtcagc gtcggctcga gtccggcccg tcgcgtccga
    14401 cggtgccgga catcgagcgg catagaatgg cgcgcgatgg cgctccgcgt cgctcccgtc
    14461 gtcccggagg gcgacgctcc cacggcggcg ccggcccctg agctcgaccg gaccgaggcc
    14521 cggctcttcg tcgaggcgct cctcctcgcc gtcacggacg cggtctgggt gtgcgacgcg
    14581 tccgggcggc tcgtcctggc gaacgatgcg ttcggcgcgg cgctcggacg accggccggc
    14641 gccctccggg gactcactcc cgaggaggtc ttttcgcccg atctggcggc gcgggtccgc
    14701 cgtgcgcaga accgcgtgat ggggacgggg gccccgctcc gggtgcagga gttcctgtat
    14761 ccggtgggct cggtcgagcg acgcctgttc gagacgtctt tcggctcgat ccgcggcccc
    14821 ggcggaaagc cctgggggac ggtggcggtc acccgcgacg tcacgcacga tcggctcgga
    14881 gacgaggagc tcctcggaac tcgcgacttc ctcttcgccc tcgtcaatgc ggtcggaccg
    14941 gggtggacct cgaatcggga ccaccgtctg accctcgtga gccgagctgc ggccgagatc
    15001 ttcggccatc cgccatcgat cgtcatcggg aaaacgccgg aacagctcct cccggaggag
    15061 gaggtgggga cgatcgtcgc ccttcaggaa gaggcgtttc ggaccggacg ggaggtgagc
    15121 cgggagctcg agcttcgcgg ccccgagggt cgagcggcct ccttcctcgt ccgcgcgttc
    15181 ccatacgtcg gcccggcgtc cgaggctctc gtggtctccc tctgcaccga cgtcacggag
    15241 atccggcggc tcggacgcgg tctcgcggcc gcgggggact tcctgtccgg cattctcgac
    15301 gccatccccg atccggtctt cgcgaaggac cgcgagcatc gctgggtctt cgtgaacgac
    15361 gcgatgtgcg ccctcgtcgg gagggaccgg gaccagcttc tctcccatct cgattccgag
    15421 ttcttcgggg ccagccaggt tcccgggatc catgagcggg acgacgaggt ctttcgaacg
    15481 ggccgggaga gcctcgtcga ggaggacctg acggtccgga caggcgagac gaagcgggtc
    15541 gtcgtgagga agtccttcca cgtggatccg gagggccggc cgctcgtcgt cggcgtgatc
    15601 cgggatgtca cgaagacgag gagcgacgag gcggcgctcc ggcgagcgaa ccggcggctc
    15661 gaagcagcga actcagagct cgaagcgttc gtcttcgcgg cctctcacga cctcgtcgct
    15721 ccgctccgcc ggatcgagtc gttctccgag ttcctcctgg aggggtgcct cgaccggctg
    15781 gagctcgacg gtcaggagct cctgcgaagg atctggagca cggcacggag cggcgagcag
    15841 ctgacggagg acctcctcac cctctcccga gcgacgcgcg gcgagctcct gcgggagcgg
    15901 atcgacctca gctccctcgc ccgcgagctc gccgacgggc tcctggagga cagcccgggg
    15961 cgtgccgtgg agatcgacgt cgcggacggt gtggtggtgg atgccgatcg ccgcctgctg
    16021 aggctgtgcc tggagaacct gctcgggaac gccctgaagt tcacgcgcgg ccgcgaggcg
    16081 cggatagagg tcggggagga agggcgagac gtcgaggggg agcgcgtcta cttcgtcagg
    16141 gacaacggcg tcggcttcga gatggcgttc gccgaccgtc tctttggtgt gttccagcgt
    16201 ctccacaggg ccgaggacta ccccggttcc ggaatcgggc tcgcgctcgt ccagcgcatc
    16261 gtccatcgac acgggggacg agtctgggcc gagagcgccg tcggccaggg aacgacgatc
    16321 cggttcacgc tcggcgtctg agctgggggg gattcgcgac gggccgcggt acgcatcgcc
    16381 cccgccagcg gcccgtctcc gggaagggaa cccggaccgc acgagctccc ggcgcaagcg
    16441 gcggcctgcg cgaggtggag agcccggtcc ttctcgttcc gcgtcagcgc ggcgcactcc
    16501 aggtcgggac cgcctcggcg atgagcgcct ggtgcttcgg ctcgaggccc gagaggtcgg
    16561 tgagctgccg gacctcgagg acgtcgccgg agccgtcggc tgcgacgttc agccggcggg
    16621 cggcgtcgcc ggcgaggccg gcggcgccgc cgaggtcacg accgcgaagc cagcgtcgct
    16681 ccccttctcg tcccagatgc cgacgcagac gcgcgcgtcc gggccggtcg ccacgatctc
    16741 gagctcgtac gtgtagtggc ccgccttcgc ggtctcgagg tcggccgcgg gaatctcgaa
    16801 cgcctggctc cgccgggtga cctcggagaa gtccccggcg gcggccacgg aggcgacgag
    16861 gacggagaac gccccctcga cccccttcgc ggtcgggagg aggacgaggg aggcgatggg
    16921 gatccggacc tcgacgcgga tccggaactt gccgtcctgc gccgggatcg gggaggccgt
    16981 cgccgagatc gggatccgcg cccggccgtc cggctggaag aggtgggcga ggacccggtc
    17041 ggccatctgc tcggcggtcg tcttctcgac gagggagctt cggacacgga cgtcgagctt
    17101 gcgccccttc acctccacgg tgaccggcac ggccttgccc gcgcccggcg gcgcagggta
    17161 tccgatcgag taccacgact cgaggtcgga ggcgacgcgg tcgacgaacc ggtcgacgtt
    17221 cccggcgccg accgccgcga cgccgccggt cttctcggcg acgaaggaga gcgcctcgag
    17281 ctcgttgtcg aggacgatct gctccttccc gccgagcggc tggtccgaga gcccgggcgc
    17341 ggagaggcgg gaccggtccg ccgacggcat cgccgcgtcg aggtcggccg gaaagacccc
    17401 gtggagcgtc acgccgttcg cgttcgccgc gcgcgccacc tcctcgagga gccgcttcga
    17461 gtcgaactgc cgggtccgcg ggtcgccgag cgcctcggca ccggcacggt ccttgagaag
    17521 gaactcgagg ccggcgtagc gcgagaaacg gtgggaaacg aggacgagga ccttccgccc
    17581 gtccatcccc ccgagcgtcg cggcgagccc cttcagcgcc gacgtcttcg ccttcatctc
    17641 gaagaacgcc tgctgcgcgg cgagctcggc cgagggcatg acgccgccga agtcgacccc
    17701 gatccgcggg tcggcggcca gggagctgaa ccactcgtcc gacg
//
LOCUS       DAOONL010000071        17323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3425_length_17323_cov_13.025481, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000071 DAOONL010000000
VERSION     DAOONL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3425_length_17323_cov_13.025481"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLP50_11180"
     CDS             complement(<1..889)
                     /locus_tag="PLP50_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52153.1"
                     /translation="MSSSGPVPRIFGPFVLTRHLGTDPLGGLYRAGTATGAQLAPFLL
                     IRVFEGAGIDQAALVPAMEEASEYLEVVRGPAVAKGAVLGIVDDVPFAGIEYVPGRTL
                     DRLFSGEGQGPIPLPVEHALLIAEKLLVALEAGKPFEKKMGAPHGFLTPGFVTISNDG
                     DVRVYGAGLGSGLLPSLKSAPIRSAFASYIAPEVLASGKPTVAGDIYSAAAILFHALT
                     GKPPAPGAGADSLAGAVLAIDGSPMPDDLGKLVARGLTKDPAQREQDVVAYRKTLGKL
                     LYGGPYAPSTFNLAFFMHQK"
     gene            complement(936..1991)
                     /gene="mltG"
                     /locus_tag="PLP50_11185"
     CDS             complement(936..1991)
                     /gene="mltG"
                     /locus_tag="PLP50_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPA52154.1"
                     /translation="MRRLLRAGLRALALLALVTALGGGAALYLVRSPYQGWTGDEIVV
                     ELPSGASARAILARLERSGVLRSRHLAGAAYRVLFPGKTLKAGEYRFTGPLSTEQVLR
                     DLVEGNVVTYRLTIPEGWTAEEIFDLVAREGLAPVEPLRELFLQPALFPEVPPDAPTL
                     EGFLGPETHLFTRSQRPREIVATLVRAWIRSLPEGFAERAGALGRTPLEAVTLASLVE
                     KETGVAGERTLVSAVYHNRLRVGMPLQCDPTVVYALKRRGLWTGDLTRDGLAVDDPYN
                     TYARGGLPPGPIASPGAAALEAAVAPASVPYLYFVAVGDGSGGHRFAVTYEEHLANVA
                     LYRRARAARETVSGASR"
     gene            complement(1988..2416)
                     /gene="ruvX"
                     /locus_tag="PLP50_11190"
     CDS             complement(1988..2416)
                     /gene="ruvX"
                     /locus_tag="PLP50_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPA52155.1"
                     /translation="MRVLGVDFGERRIGLAVSGPSANVVVPVGVVHRRSDAQALEAVA
                     AAARERDAALVVVGVPVRPEGGGESPVAARARSFAKKLETATGLTVELHDEAFTSVSA
                     EGALREAGLRRDRRSGVVDAEAAAELLRDWLSSRPDGTVG"
     gene            2547..3731
                     /locus_tag="PLP50_11195"
     CDS             2547..3731
                     /locus_tag="PLP50_11195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPA52156.1"
                     /translation="MKNVLVLSATRTPIGTFLGSLAPLTASEIGARALKSCLERTEIP
                     GYRVDQVLLGNVLQAGQGQAPARQALIAAGLPRSTGAVTIHKVCGSGMRAVMDGANAI
                     RAGEHEIVLAGGMESMSNAPYLLPKGRTGMRMGHGQLVDGMIHDGLWDPYKNVHMGNC
                     AEMCAAKYAFTREAQDAFSMESYQRARRANDSGDFRAEMVPVSIEGKKGTVVVDRDEE
                     PFATPLEKLEKMGSLKPAFQKDGTVTAANSSKIDDGAAALLLATEAVAQETGAKPLGR
                     IVSFASHAHEPEWFTTAPSFAAKKALEKAGIPLSQIDLFEVNEAFAVVTMAFVKDLEV
                     DPNRVNVNGGAVAMGHPIGASGARILVTLLHALRARKKKYGLAAICIGGGEATAMVVE
                     AL"
     gene            3728..4441
                     /locus_tag="PLP50_11200"
     CDS             3728..4441
                     /locus_tag="PLP50_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPA52157.1"
                     /translation="MIDKVVLSSSHHEAAARAVADIPSGATIAVGGFGLCGIPENLIQ
                     ALADRDVKDLVCVSNNCGVDDWGLGILLGKKMIRKMVSSYVGENAEFERQYLAGELEL
                     EFCPQGTLAERLRAGGAGIAGFYTPTGVGTVVAEGKETKLFDGREFVLERGIVTDYAL
                     VAAWKGDRHGNLVYRKTAKNFNPMCATAGKITIAEVEQLVDVGEIDPDEVDTPGVFVN
                     RLIVAPRAKRIERRTVRKD"
     gene            4452..5117
                     /locus_tag="PLP50_11205"
     CDS             4452..5117
                     /locus_tag="PLP50_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPA52158.1"
                     /translation="MAKLERDGIVARAAQELRDGMYVNLGIGMPTLVANAVPPGMEVV
                     FQSENGILGMSGFPTESEVDADLINAGKETVTVIPGASFFSSADSFAMIRGGHIDLCI
                     LGAMQVDAEGSLANWMIPGRLVKGMGGAMDLVAGARRVIVTMEHVAKKGDKKILNRCT
                     LPFTGKRCVHRIVTDLAVLDVKPDGAGLVLVERAPGVTVEEIQAATEPPLEVRGDVPE
                     MKL"
     gene            5180..7327
                     /gene="lptD"
                     /locus_tag="PLP50_11210"
     CDS             5180..7327
                     /gene="lptD"
                     /locus_tag="PLP50_11210"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPA52159.1"
                     /translation="MLLFAAPAAGQSSFESDPFASRFRERPNLDIKFRVPEAGGVIKV
                     SVSDGQGGRQSLLSETVYEVQAPKGGVVTLEYQDVRLTADWVRADTKTKQVVAEGDVV
                     LEQGTTRLLGLRLEMDLTDKTGVLTDGSAELAGILVRGATLAKTGARTFEIRDGSVTA
                     CEGDDPAWEFRVKDGAVTLDDYAKLKSVVFRLGGVPLLYTPYLLWPALRDRASGFLVP
                     GVGYSTSRGGFLGLSYFQVLGRSADATITTDLYTKEYFGLGTELRLRPTTGTSFAGSY
                     YTVVDPDRRWQWKTWGTLSADDLAPRTRAVVNWTDFSDLAFFQRYDRDFALTSARTVG
                     QKAFVTRSEGPVAFNLRLERETALYGSSEVVLERKPVLEARLRPTGLFGNALFVEAQG
                     SAGFLRSIRPAGQPAGDYERFDLFPKLSAPISPLPWLSVNAEAGYRVTRYGASVSEDR
                     RSLTTEPYTRGSANAGLEVTGPSFSRVFDVKLGKFRKLKHVIEPRADYSYQTDPEDLS
                     RTPVFDEVDTILPSHQVRYSLVQRLLAKEEKGASREIASFELSRTHYFELPVGAATTV
                     RDSPLDAVLRVNAGSALNFDARATWDLKEGVVTSSSVSANVGSAERFLRLSFFDSRPV
                     GAPTPSTQLRFGGGSSILPKRLRLDVEANYDVERGKMLEWRSLVTWEASCFKILGEYR
                     DLRIGAIPTRDYRIGLNLKNIGSFLDFTGRVGN"
     gene            7365..8210
                     /gene="rfbD"
                     /locus_tag="PLP50_11215"
     CDS             7365..8210
                     /gene="rfbD"
                     /locus_tag="PLP50_11215"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPA52160.1"
                     /translation="MSELILGAGGMLARGLAALRPQATALGVAELDVTDAAALARVIV
                     PGVSVVWNAAADTRVDNCESDPGHVAVNDVAVGVIARLCREAGALLVHVSTDYVFDGK
                     PGRPWREDDPVDPVNAYGRGKLGGERRALASGAEVLVVRTSWVFAPGGVNFVDTILGL
                     AASGREELKVVDDQRGRPTYAPDLARALVKLVSVGARGLVHFANSGETTWYGLAREVL
                     ARAGFAGVRVAPCASDEFPRPAARPANSVLDTSRYEELTGEAPRHHLEALDERLRIRA
                     EAARR"
     gene            8207..9544
                     /locus_tag="PLP50_11220"
     CDS             8207..9544
                     /locus_tag="PLP50_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HPA52161.1"
                     /translation="MSLDATEVRRKAVHVGMASFALLLRWLDVRGAALCALAAFAFNL
                     VVLPRLGGRSLFREGDRARGYPVGILLYPLVVLALVLLFPRRLDLAAAGWGFLAFGDG
                     FATLVGASLGGRRLPWNPRKSWSGLLGYVVFGCLGATALSSFVAGQLPGPAGLVAFLL
                     AALVGAVLESLPSELDDNIVPPLAAAGALALLLPALPNAALLLEPDALRRLAVGVGVN
                     VALPALAAALGVVRPSGAVAGGIVGALVLGLGGLGPYALLWAFFAGGTLATRFGRARK
                     EAMGKAEEAGGRRSAANVLANCSVAAFLVACAALTPAASSLLHLAAAAAFATALMDTV
                     GTEVGQAIRTPTVLLPDLRRVPPGTDGAVSIAGTLAGLLAAALLGLVGALFGLYEPAA
                     LPVVVVAAFAGTVVESLLGRDGASWRVTNGHVLNLFNTLAGAAVAALIARFVL"
     gene            complement(9726..10124)
                     /locus_tag="PLP50_11225"
     CDS             complement(9726..10124)
                     /locus_tag="PLP50_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HPA52162.1"
                     /translation="MKDSPVALQTTTPLSFADAVARAREELAREGFGVLTEIDVAATL
                     KKKLDVDREPYLILGACHPPSAHKALSAVPEVGVLLPCNVTVSVEGGTTVVRAMDPAG
                     VMGILGSKELAEVGADIGARLRRVVDAFRA"
     gene            complement(10210..10410)
                     /locus_tag="PLP50_11230"
     CDS             complement(10210..10410)
                     /locus_tag="PLP50_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPA52163.1"
                     /translation="MPLYEYRCDECGRTFEELRPSSEADTAIDCPSCESRRTSRKLSS
                     FASGASSSGSAKGGSGCGTRFT"
     gene            complement(10874..11242)
                     /locus_tag="PLP50_11235"
     CDS             complement(10874..11242)
                     /locus_tag="PLP50_11235"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPA52164.1"
                     /translation="MRKAASLPKEQLMEGIASFLKVLADPTRLRILHALQGGERCVSD
                     LLAELPCTQANVSKHLALLRTAGIVEFRRQGTQVYYRIADPAVFAICGVVCGAMQRSL
                     DEKRVELKAGKAALAAGARA"
     gene            complement(11368..11652)
                     /locus_tag="PLP50_11240"
     CDS             complement(11368..11652)
                     /locus_tag="PLP50_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52165.1"
                     /translation="MRMLMIVVDDGKKEELEVLLRKAGVTGWTEIAGASGMGTTGPRL
                     GSAAFPKTSAVLFTIVEDERARTLAEEIKSYCAECGERLRIASWAVEELL"
     gene            complement(11640..11846)
                     /locus_tag="PLP50_11245"
     CDS             complement(11640..11846)
                     /locus_tag="PLP50_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPA52166.1"
                     /translation="MNLNEWLRAIAGFFVLLSVVLAVTVDQRFLWFTGFVGLNLLQSA
                     FTKWCPAIAMLRKLGVKEGGACGC"
     assembly_gap    11965..12064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12065..15397)
                     /locus_tag="PLP50_11250"
     CDS             complement(<12065..15397)
                     /locus_tag="PLP50_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA52167.1"
                     /translation="MSEPRDEAAGKAAEGAAGPTPPPSHAGTKREALRLKMTRRPVGA
                     SGRIAKAFLESKLTPLIVVASLLVGAFAVMVTPREEEPQIKVPMIDVFLGMPGATAQE
                     VERRLTSPLEKAIYEIPNVEYVYSTTQPSGGMIIVRFLVGTDPDQAAVRVHTKLAEKA
                     SELPPGAMPPLVVPRGIDDVPVLAYTLSSADASPMQLKEVAEELKVELTKHPRVAQVT
                     VLGGEKRAVKVSFDRDRLAAHQVSLLQAYMALSGANWRLPAGSFATSDAETQVEVGSL
                     FRSAEEVGNAVVAVWNGRPVFLRDVATVTDGADEPSQYVWHMTGAGGTHGAGADTAAV
                     TVSVAKKPGTNAVELVEELDAKVAGLKGKVVPSNVALTKTRDYGHTADEKSNELIFHM
                     ALATFSVVLLMAFALGRREAVVVLVAVPMTLALTLASSYLFGYTLNRVTLFALIFAIG
                     ILVDDAIVVVENIHRHYELGWGPPRLTTVYAVDEVGNPTILATFAVVGALLPLVFVSG
                     LMGPYMAPIPINASAAMIFSLFVAFVVSPWLTFKLFRKYAEEHASRSAFESHAATPQE
                     DKLHRFYQRLFEPLLTSPAKSWALMGGVVVLLLLSMSLVYFKVAKVKMLPYDNKSEIQ
                     VVVDMPEGTTLEATNAVARDLARKVRNEPEVTDVQVYVGTTAPFNFNGLVRHYFLRSG
                     PLVADLQVNLLPKQERKRDSHTFAKDVRTLLLPIAKRHGANIKVTEVPPGPPVLSTMV
                     AEIYGPDMAKRIDLARQVRTIFEETPGIVDVDWHVEDEAPKVELVVDREKATRAGVTP
                     EVVTRTLRVALNGADAGLLRDESSRKPVPIVLRLDRSQRSSLDGLLQTAVHGGTGRLV
                     PLRELVTVKETKSERFIYHKNLQPVTYVLAEMAGVAEAPVYGILDMGERIEALKAPDG
                     SPLQVMSTTLPTDSTKYAMKWDGEWHITYEVFRDMGIAFAAVLVLIYFLVVGWFRSFV
                     TPLIIMAPIPLTLIGILPAHGLFGIFFTATSMIGFIALAGIIVRNSILLVDFINLELE
                     MGEKLEDAVIKAGAVRFRPIALTAAALVVGGGVILLDPIFQGLAVALIAGVVVATALT
                     LVVIPLLYYMYLKAVGVEKALPP"
     gene            complement(15394..16491)
                     /locus_tag="PLP50_11255"
     CDS             complement(15394..16491)
                     /locus_tag="PLP50_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52168.1"
                     /translation="MTPRKTLSLVAITVAGTLALSACGGGKKAGEAAAPAPVTAALAK
                     AESRELPQRIELSGTVEAGKSAMVSSRVMAAVVAVPVKEGDLVAQGQLLVEIDPQTAK
                     GQESQARGALAQAKAALALAERNWQRFQALQKSAAASELEVDLARMQYEQAKGAVEQA
                     TGAVEAASSVARESRVVAPFAGRVARKMVDPGDLAAPGRPLVVVESTAGRRLVLSVPE
                     SVAVASKLKTGALLPVSLDAMPGATLAGRVVEMSAGADPASHSFTARVEVSGAQVPTG
                     VTGRALLETGRRTAVVVPAAAVLRQGAMSLVVVKDEEGKARTRAVTLGTRDGDLVEVL
                     SGLKGGEDVLVGLAQAPADGAPVNASSEVGQ"
     gene            complement(16545..>17323)
                     /locus_tag="PLP50_11260"
     CDS             complement(16545..>17323)
                     /locus_tag="PLP50_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA52169.1"
                     /translation="SYVEQGMLVRSELLRAEVELARVEDMLQDAKGKARVANANLAFR
                     IGVAQDSTWDLAPLPGPKPLDGDAASWVSSAGSRRDLSAARSLLAAGELEEKVKKAAW
                     LPKVGVMARWDLHDRNLFGASGSAGTVMAFASVNLFQGGADKAALVAAREEARAGRED
                     VARFADGVALEVRQAYEEAATARARHATAVKALEAARESERITDERFRSGVVKTIDLL
                     DAATARREAETRELVARADAHATALRLAVKAGKAPETAIP"
BASE COUNT         2352 a         6065 c         6348 g         2458 t
ORIGIN      
        1 acttctggtg catgaagaag gcgaggttga acgtggaggg ggcgtagggg ccgccataga
       61 ggagcttgcc gagcgtcttg cggtaggcga cgacgtcctg ctcgcgctgg gccgggtcct
      121 tggtgaggcc gcgcgccacg agcttgccga ggtcgtcggg catcgggctc ccgtcgatgg
      181 cgaggacggc tcccgcgagg gagtccgcgc ccgcgccggg ggccggcggc ttgccggtga
      241 gggcgtggaa gaggatcgcg gccgccgagt agatgtcccc ggccaccgtc ggcttgcccg
      301 acgcgaggac ctccggcgcg atgtacgagg cgaacgcgga gcggatcgga gcggacttca
      361 gcgaagggag gagcccggag ccgagcccgg cgccgtagac cctcacgtca ccgtcgttcg
      421 agatcgtgac gaagcccggc gtcaggaagc cgtgcggcgc ccccatcttc ttctcgaacg
      481 gcttgcccgc ctcgagggcg acgagcagct tctcggcgat caggagcgcg tgctcgacgg
      541 ggagcgggat cggcccctgc ccctcgccgg agaagaggcg gtcgagcgtc cgccccggca
      601 cgtactcgat gccggcgaac ggcacgtcgt ccacgatgcc gaggaccgcg cccttggcga
      661 ccgcgggccc gcggacgacc tccaggtact cggaggcctc ctccatcgcg gggacgaggg
      721 cggcctggtc gattccggcc ccttcgaaga cgcggatcag gaggaagggc gcgagctgcg
      781 cccccgtcgc cgtcccggcc cggtagaggc cgccgagcgg gtcggttccg aggtggcggg
      841 tgaggacgaa ggggccgaag attctgggga ccgggccgct gctgctcatc tgctctccgc
      901 ttccggatgg cgttccggag aacgaaaagt ctacctcagc gggatgcccc ggagacggtt
      961 tcccgcgcgg cgcgggcgcg cctgtagagc gccacgttcg cgaggtgctc ctcgtacgtc
     1021 accgcgaagc gatgcccccc cgagccgtcg ccgaccgcga cgaagtagag gtacggcacc
     1081 gaggcggggg ccacggccgc ctccagggcg gcggcgccgg ggctcgcgat ggggcccggc
     1141 gggaggccgc cgcgcgcgta ggtgttgtac gggtcgtcga cggcgaggcc gtcgcgcgtc
     1201 aggtcccccg tccagagccc gcgtcgcttc agggcgtaga cgacggtcgg gtcgcactgg
     1261 agaggcatgc cgacccggag gcggttgtgg tagacggccg acacgagcgt ccgctccccg
     1321 gcgacgcccg tctccttctc gacgagtgac gccagcgtga cggcctcgag cggcgtccgg
     1381 ccgagggcgc cggcccgctc ggcgaagccc tccggcaggg agcggatcca ggccctcacg
     1441 agcgtggcga cgatctcgcg ggggcgctgc gagcgggtga agaggtgcgt ctcggggccc
     1501 aggaacccct ccagggtcgg ggcgtcgggc ggaacctcgg ggaagagagc cggctggagg
     1561 aacagctcgc ggagcggctc gaccggagcg agcccctccc gcgcgacgag gtcgaagatc
     1621 tcctccgcgg tccatccttc ggggatcgtc aggcgatagg tgacgacgtt cccctcgacg
     1681 aggtcgcgga ggacctgttc ggtcgaaagg ggacccgtga agcggtactc cccggccttc
     1741 agggtcttcc ccgggaagag gacgcggtag gcggcgccgg cgaggtggcg gctccgaagg
     1801 acgccggagc gctcgaggcg ggcgaggatc gcccgcgccg aggcgcccga agggagctcc
     1861 accacgatct cgtcgcccgt ccagccctgg tacgggctcc tcacgaggta gagcgcggcg
     1921 ccgccgccca gggccgtgac gagcgcgagg agggcgagcg cgcggaggcc ggcgcggagg
     1981 aggcgcctca cccgaccgtc ccgtccggcc gggaagagag ccagtcgcgg aggagctcgg
     2041 cggcggcctc ggcgtcgacc accccggagc gccggtcccg gcggaggccg gcctcgcgga
     2101 gggcgccctc ggccgagacg gaggtgaagg cctcgtcgtg gagctcgacg gtgagtccgg
     2161 tcgcggtctc gagtttcttc gcgaagctcc gcgcccgcgc ggcgacaggg ctctcgccgc
     2221 cgccttcggg ccgcacgggg acgccgacga cgacgagcgc cgcgtctctc tccctggcgg
     2281 cggcggcgac cgcctccagc gcctgcgcgt cgctcctccg gtgcacgacg ccgaccggga
     2341 cgacgacgtt cgccgacggc ccggaaacgg cgagaccgat cctccgctcg ccgaagtcga
     2401 ccccgaggac gcgcacggcg cgaggatagg ggggcgcctc cgggggtgtc catccccgga
     2461 gagaccgtcc gggatcggat gagtagaatc cttcggtccc ccggaggccc ctgcgtggcc
     2521 cgggggaggt acggagcaag cgaacgatga agaacgtcct cgtcctctcg gcgacccgaa
     2581 cgccgatcgg aaccttcctg gggtctctcg cccccctcac cgcctccgag atcggcgccc
     2641 gggcgctcaa gtcgtgcctc gagcggaccg agatccccgg ctaccgggtc gaccaggtcc
     2701 tcctggggaa cgtcctccag gccggccagg ggcaggcccc ggcccggcag gcgctgatcg
     2761 ccgccggcct ccccaggtcg accggtgccg tgacgattca caaggtctgc ggctccggga
     2821 tgcgggccgt catggacggc gcgaacgcga tccgggccgg agagcacgag atcgtcctgg
     2881 cgggcggcat ggagtcgatg tcgaacgccc cctacctcct cccgaagggg cggacgggga
     2941 tgcggatggg gcacgggcag ctcgtcgacg ggatgatcca cgacggcctc tgggacccct
     3001 acaagaacgt ccacatgggc aactgcgccg agatgtgcgc cgcgaagtac gccttcacgc
     3061 gcgaggcgca ggacgccttc tcgatggaga gctaccagcg ggcgcgccgg gcgaacgact
     3121 cgggcgactt ccgcgccgag atggtcccgg tctcgatcga ggggaagaag gggacggtcg
     3181 tcgtcgaccg cgacgaggag ccgttcgcga ccccgctcga gaagctcgag aagatgggct
     3241 cgctgaagcc cgccttccag aaggacggga ccgtcacggc cgcgaactcc tcgaagatcg
     3301 acgacggggc ggcggcgctc ctcctggcga ccgaggcggt cgcccaggag acgggggcga
     3361 agccgcttgg ccggatcgtc tcgttcgcct cccacgccca cgagccggag tggttcacga
     3421 cggccccgtc gttcgccgcg aagaaggcgc tcgagaaggc cggcatcccg ctctcgcaga
     3481 tcgacctctt cgaggtgaac gaggcgttcg ccgtcgtcac gatggcgttc gtgaaggacc
     3541 tggaggtcga cccgaaccgg gtcaacgtga acggcggcgc cgtcgccatg gggcacccga
     3601 tcggggcctc cggcgcgcgg atcctcgtca cgctcctcca cgcgctgagg gcccggaaga
     3661 agaagtacgg cctcgcggcg atctgcatcg gcggcggcga ggcgaccgcg atggtggtgg
     3721 aggcgctgtg atcgacaagg tcgtcctctc ctcctcgcac cacgaggcgg ctgcccgcgc
     3781 cgtggccgac atcccctcgg gggcgacgat cgccgtcggc ggcttcggcc tttgcggtat
     3841 ccccgagaac ctgatccagg ccctcgccga ccgcgacgtg aaggacctcg tctgcgtctc
     3901 gaacaactgc ggcgtcgacg actggggcct cgggatcctc ctcggcaaga agatgatccg
     3961 gaagatggtc tcctcctacg tcggcgagaa cgccgagttc gagcggcagt acctggcggg
     4021 cgagctggag ctcgagttct gcccgcaggg gacgctcgcg gagcgtctcc gggccggcgg
     4081 cgccggcatc gccggcttct acacgccgac gggcgtcggg accgtcgtcg ccgaggggaa
     4141 ggagacgaag ctgttcgacg ggcgcgagtt cgtcctcgag cggggcatcg tcaccgacta
     4201 cgccctcgtc gccgcctgga agggcgaccg gcacgggaac ctcgtctacc ggaagacggc
     4261 gaagaacttc aacccgatgt gcgccaccgc ggggaagatc acgatcgccg aggtcgagca
     4321 gctcgtcgac gtcggcgaga tcgacccgga cgaggtcgac acccccggcg tcttcgtgaa
     4381 ccgcctgatc gtcgctcccc gcgccaagcg gatcgagcgg cgaacggtga ggaaggactg
     4441 agggggcggc gatggcgaag ctcgaaaggg acggaatcgt cgcccgggcc gcgcaggagc
     4501 tgcgcgacgg gatgtacgtc aacctcggca tcgggatgcc gacgctggtc gcgaacgcgg
     4561 tgccgcccgg gatggaggtc gtcttccagt cggagaacgg catcctcggg atgagcggct
     4621 tcccgaccga gtccgaggtg gacgcggacc tgatcaacgc cgggaaggag acggtcaccg
     4681 tcatccccgg cgcctcgttc ttctcgtccg ccgactcgtt cgcgatgatc cgcggcggcc
     4741 acatcgacct ctgcatcctc ggcgcgatgc aggtcgacgc cgaggggtcc ctcgcgaact
     4801 ggatgatccc ggggcgcctc gtgaagggga tggggggcgc gatggacctc gtcgccggcg
     4861 cccgccgcgt catcgtgacg atggagcacg tcgcgaagaa gggcgacaag aagatcctga
     4921 accgatgcac gctccccttc accgggaagc gctgcgtcca ccggatcgtc accgacctcg
     4981 ccgtcctcga cgtgaagccc gacggggcgg gcctcgtcct cgtcgagagg gcgccgggtg
     5041 tgaccgtcga ggagatccag gcggcgacgg agccgcccct cgaggtccgc ggcgacgttc
     5101 ccgagatgaa gctctgaacc ggggctcacg acggtttctc acgccgtggc gggccccctt
     5161 cgcggggctc gccgcggcgt tgctcctctt cgccgcgccc gccgcggggc aatcctcctt
     5221 cgagagcgac ccgttcgcct cgcgcttccg cgagcggccg aacctcgaca tcaagttccg
     5281 cgtgccggag gcggggggcg tcatcaaggt ctcggtctcc gacggtcagg gggggcggca
     5341 gagccttctc tcggagacgg tctacgaggt ccaggccccg aaggggggcg tcgtcacgct
     5401 ggagtaccag gacgtccggc tgacggccga ctgggtgcgg gccgacacga agacgaagca
     5461 ggtcgtcgcc gagggggatg tcgtcctcga gcaggggacg acccgcctcc tgggcctgcg
     5521 cctggagatg gacctgaccg acaagaccgg ggtcctgacc gacgggagcg cggagctcgc
     5581 ggggatcctc gtccgcgggg cgacgctcgc gaagaccggg gcgcggacgt tcgagatccg
     5641 ggacggctcg gtcacggcct gcgaggggga cgacccggcg tgggagttcc gcgtgaagga
     5701 cggcgccgtc acgctggacg actacgcgaa gctgaagtcg gtcgtcttcc gccttggcgg
     5761 cgtgccgctc ctctacaccc cgtacctcct ctggccggcg ctgcgcgacc gggcctcggg
     5821 cttcctcgtc ccgggggtcg gctacagcac gagccggggc ggcttcctcg gcctctcgta
     5881 cttccaggtc ctcgggaggt cggccgacgc gacgatcacg accgacctct acacgaagga
     5941 gtacttcggc ctcgggaccg agctgcgcct gcggccgacc acggggacct ccttcgccgg
     6001 ctcctattac accgtcgtcg accccgaccg gcgctggcag tggaagacgt gggggacgct
     6061 ctcggccgac gacctcgcgc cccggacgcg cgccgtcgtc aactggaccg acttctcgga
     6121 cctcgcgttc ttccagcgct acgaccggga cttcgcgctc acgtcggccc ggaccgtcgg
     6181 gcagaaggcg ttcgtcaccc gctcggaagg gccggtcgcg ttcaacctcc ggctcgagcg
     6241 cgagacggcg ctctacggct cgagcgaggt cgttctcgag cgcaagcccg ttctcgaggc
     6301 gcgactccgg cccacgggcc tcttcggcaa cgccctcttc gtcgaggctc agggctcggc
     6361 gggattcctc cgctcgatcc ggccggcggg gcagccggcc ggggactacg agcggttcga
     6421 cctcttcccg aagctctcgg cccccatttc gccgctcccg tggctctcgg tcaacgccga
     6481 ggccggctat cgagtcacgc ggtacggcgc ctctgtatcc gaggaccgtc gatcgctcac
     6541 cacggagccc tacacgcgcg gctccgcgaa cgccggcctc gaggtgaccg ggccgtcgtt
     6601 ctcccgcgtc ttcgacgtga agctcgggaa gttccgcaag ctcaagcacg tcatcgagcc
     6661 gcgcgccgac tactcctacc agaccgaccc ggaggacctc tcccggaccc ccgtcttcga
     6721 cgaggtcgac acgatcctcc cgtcgcacca ggtgcgctac tcgctcgtcc aacggctcct
     6781 cgcgaaggag gagaagggcg cctcccgcga gatcgcctcg ttcgagctct cgcgcacgca
     6841 ctacttcgag ctccccgtcg gcgcggccac gaccgtccgc gactcgcccc tcgacgcggt
     6901 cctccgcgtc aacgcgggct ccgccctcaa cttcgacgcg cgggcgacgt gggacctgaa
     6961 ggagggcgtc gtcacctcct cgagcgtgtc ggcgaacgtc ggctcggccg agcgcttcct
     7021 ccggctctcc ttcttcgaca gccgccccgt cggggcgccg acgccctcga cgcagctccg
     7081 cttcggcggg ggctcctcga tcctgccgaa gaggctccgc ctcgacgtcg aggcgaacta
     7141 cgacgtcgag cgcgggaaga tgctcgaatg gcggagcctc gtgacctggg aggcgtcgtg
     7201 cttcaagatc ctcggcgagt accgcgacct gcggatcggc gcgatcccga cgcgcgacta
     7261 ccggatcggg ctgaacctga agaacatcgg gagcttcctc gacttcaccg ggcgggtggg
     7321 gaactgacgc cgcccccgag gccggctccg cggagggcga cggagtgagc gagctgatcc
     7381 tcggcgccgg cggcatgctc gctcgcggcc tcgcggcgct gcggccgcaa gcgaccgcgc
     7441 tcggcgtcgc cgagctggac gtcaccgacg cggccgccct cgcccgcgtg atcgtccccg
     7501 gcgtctcggt cgtctggaac gcggcggccg acacgcgagt cgacaactgc gagagcgacc
     7561 ccggccacgt cgccgtgaac gacgtcgccg tcggcgtcat cgcgcgcctc tgccgcgagg
     7621 cgggggcgct cctcgtccac gtctcgacgg actacgtctt cgacggcaag cccggccggc
     7681 cctggcgcga ggacgacccc gtcgacccgg tcaacgccta cggccgcggc aagctcggcg
     7741 gcgagcgccg ggcgctggcg agcggggcgg aggtcctcgt cgtccggacc tcgtgggtct
     7801 tcgcgccggg cggcgtcaac ttcgtcgaca cgatcctcgg cctcgccgcg tcgggccggg
     7861 aggagctgaa ggtcgtcgac gaccagcggg gccgcccgac gtacgcgccg gacctcgccc
     7921 gggcgctcgt gaagctcgtc tcggtcggcg cgcgcggcct cgtccacttc gcgaactccg
     7981 gcgagacgac ctggtacggc ctcgctcgcg aggtgctcgc ccgcgccggc ttcgcgggcg
     8041 ttcgcgtcgc cccgtgcgcg agcgacgagt tcccgcggcc ggccgcgcgc ccggcgaact
     8101 ccgtcctcga cacctcccgc tacgaggagc tgaccggcga ggcgccccgc catcacctcg
     8161 aggcgctcga cgagcgtctc cgcatccgcg cggaggcggc ccggaggtga gcctcgacgc
     8221 cacggaggtg aggaggaagg ccgtccacgt cgggatggcc tcgttcgcgc tcctcctccg
     8281 gtggctcgac gttcggggtg ccgctctctg cgcgctcgcg gcgttcgcct tcaacctcgt
     8341 ggtcctcccg cggctcggcg gacggagcct tttccgcgaa ggcgaccgcg cccgcggcta
     8401 ccccgtcggc atcctcctct acccgctcgt cgtcctcgcg ctcgtcctcc tcttcccgcg
     8461 ccgcctcgac ctcgcggcgg ccgggtgggg tttcctcgcg ttcggcgacg gcttcgcgac
     8521 gctcgtcggc gcgtcgctcg gcgggcggcg gctcccctgg aacccgcgga agagctggag
     8581 cggtctcctc ggctacgtcg tcttcgggtg cctcggcgcg acggcgctct cctcgttcgt
     8641 cgccggacag ctcccggggc cggccgggct cgtcgcgttc ctcctggccg cgctcgtcgg
     8701 cgcggtcctc gagagcctcc cgtccgagct ggacgacaac atcgtgcctc ccctggcggc
     8761 ggcgggcgcg ctcgctctcc tcctccccgc cctcccgaac gccgctctcc tcctcgagcc
     8821 ggacgccctc cggcgcctcg ccgtcggcgt cggggtgaac gtcgccctgc cggcgctggc
     8881 cgccgccctc ggcgtcgtcc gcccctcggg ggccgtggcg ggcgggatcg tcggcgccct
     8941 cgtcctcggc ctcggcggcc tcgggccgta cgcgctcctc tgggccttct tcgccggcgg
     9001 aacgctcgcg acgcgattcg gccgggcgcg gaaggaggcg atggggaagg ccgaggaggc
     9061 gggagggcgg cgaagcgcgg cgaacgtcct cgcgaactgc tcggtcgcag cgttcctcgt
     9121 cgcctgcgcg gcgctgacgc cggccgcctc ttcgctcctg cacctcgcgg cggccgcggc
     9181 gttcgccacg gcgctgatgg acacggtcgg gaccgaggtg ggacaggcga tccggacgcc
     9241 gaccgtcctc ctccccgatc tccggcgcgt cccgccgggg accgacggcg ccgtctcgat
     9301 cgcggggacg ctcgcgggcc tcctcgcggc ggcgctcctc ggcctggtcg gagctctctt
     9361 cgggctctac gagcccgccg ccctccccgt cgtcgtcgtc gccgcgttcg ccggcaccgt
     9421 cgtcgagagc ctgctggggc gcgacggggc gtcctggcgc gtcacgaacg gccacgtcct
     9481 gaacctcttc aacacgctcg ccggcgccgc ggtcgcggcg ctgatcgcgc gcttcgtcct
     9541 ctgagcggtc cgggaaggat tccgtcgacg ccccggcgcg ccccggggcg gcgctgcccg
     9601 gcgtcgggga tcgcctgccc gggggagcgg accggagtcg tcttcggtga gatggactct
     9661 acggatcgcc cgcggtcgcc gcgatgacgt tcgtcacgcg gaaggcgggc gggaaggggc
     9721 gttcctcagg cgcggaaggc gtcgacgacg cgccggaggc gcgcgccgat gtcggcgccg
     9781 acctcggcga gctccttcga gccgaggatc cccatgacgc cggccgggtc catcgcgcgg
     9841 acgacggtcg tccctccctc cacggagacc gtgacgttgc aggggaggag gacgccgacc
     9901 tccgggacgg ccgagagggc cttgtgcgcc gacggcgggt ggcaggcgcc gaggatgagg
     9961 tagggctcgc ggtcgacgtc gagcttcttc ttcagcgtcg ccgccacgtc gatctcagtc
    10021 aggacgccga agccttccct cgcgagctcc tcgcgcgcgc gggcgacggc gtcggcgaag
    10081 gagaggggcg tggtcgtctg gagggcgacg ggggaatcct tcatcgatgc tcctttcgcg
    10141 cccgcgggcg ccgttcgggt cggcgtggag atggtgcggt gggggcccga cgggcccccc
    10201 gggctaccct cacgtgaagc gggtgccgca gcccgagcct cccttggccg agccggagga
    10261 gctcgctccg gaagcgaagc tcgagagctt gcgggaggtg cggcgcgact cgcaggaggg
    10321 gcagtcgatc gcggtgtcgg cctcgctcga ggggcggagc tcctcgaagg tccgcccgca
    10381 ttcgtcgcat cggtattcgt agagcggcat cgtctcgcct cgggggagga gccgtggggc
    10441 gggcctcccg ttctgtcgtg cgccgagccg gccggctcag cagcctccgg tcagcttctc
    10501 ccagacgtgg gccgggaggc cggaggggta gatctgatcg gttcggtaga cgttccagca
    10561 gccctggcac cgccagagca ggactccccc tcacgtcggg acggggagga cctcctcgcc
    10621 gcagacgcgg cagcggcctt cctcgacgct caccggggag ctcgcggtct cgcccttcat
    10681 ctctctcctc gttcgtccgc cttcggggcg ggcgccgaat tcgtctcgtt cgtcgtcccg
    10741 ggtttcgtcg cgcagctccg gccgccggcc ggattgggga ccgctcacgt cgggacgccg
    10801 agcctcggga ggacgacgta ctgcaggagc acccagacga cgacgaagcc gcccacgagc
    10861 aggaggtcgg agctcatgcc cgcgccccgg cggcgagcgc cgccttcccc gccttcagct
    10921 cgacccgctt ctcgtcgagg ctcctctgca tcgcgccaca gacgacccca cagatcgcga
    10981 agaccgcggg gtccgcgatc cggtagtaga cctgcgtccc ctgccttcgg aactcgacga
    11041 tccccgccgt ccggaggagg gccaggtgct tcgagacgtt cgcctgcgtg cacggcagct
    11101 ccgcgaggag gtcgctcacg caccgctccc cgccctggag ggcgtggagg atccggagcc
    11161 gggtggggtc ggcgagcacc ttcaggaagc tcgcgatccc ctccatgagc tgctccttcg
    11221 ggagcgaggc ggccttcctc atgaccatat caccatacgg taatataaca tccgccgacg
    11281 ggatcgtcaa gcggcgatcg ccggcgggcc gggcgccggg gcgcccggcc gcgtcccggc
    11341 cgtacgcgtg tgcccggccg tcggggctca gaggagctcc tccaccgccc aggaggcgat
    11401 cctgaggcgc tcgccgcact cggcgcagta cgacttgatc tcctctgcga gagtgcgggc
    11461 gcgctcgtcc tccacgatcg tgaagaggac ggccgaggtc ttcgggaacg cggccgagcc
    11521 gaggcggggg ccggtcgtcc ccatgccgga ggctcccgcg atctcggtcc agccggtgac
    11581 gccggccttc cggaggagca cctcgagctc ctccttcttc ccgtcgtcga cgacgatcat
    11641 cagcatccgc acgcgccgcc ctccttcacg cccagcttcc ggagcatcgc gatcgcgggg
    11701 caccacttcg tgaaggccga ctggaggagg ttcaggccga cgaagccggt gaaccagagg
    11761 aagcgctggt cgaccgtcac cgccaggacg acggagagga ggacgaagaa gccggcgatc
    11821 gcccggagcc attcgttgag gttcatgtcg gtgtctcctt cgggtcgtgt tcgttcgggc
    11881 gggacgcccg gtcaggcggc ctcgtcccgg ggcgggagcg ccttctcgac gccgaccgcc
    11941 ttcaggtaca tgtagtagag gagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnngggcgg gagcgccttc tcgacgccga ccgccttcag gtacatgtag tagaggagcg
    12121 ggatgacgac gagggtcagg gcggtcgcga cgacgacgcc cgcgatgagc gccacggcga
    12181 gcccctggaa gatcgggtcg aggaggatga cgccgccgcc gacgacgagg gccgcggccg
    12241 tgagcgcgat cgggcggaag cggacggcgc ccgccttgat cacggcgtcc tcgagcttct
    12301 cgcccatctc gagctcgagg ttgatgaagt cgacgaggag gatggagttg cggacgatga
    12361 tcccggcgag cgcgatgaac ccgatcatcg acgtcgccgt gaagaagatc ccgaagaggc
    12421 cgtgcgccgg gaggatgccg atgagcgtga gcgggatcgg cgccatgatg atgagcggcg
    12481 tcacgaagct ccggaaccag ccgacgacga ggaagtagat caggacgagg acggccgcga
    12541 aggcgatccc catgtcgcgg aagacctcgt acgtgatgtg ccactcgccg tcccacttca
    12601 tcgcgtactt cgtggagtcg gtcgggagcg tcgtcgacat gacctggagc ggggagccgt
    12661 cgggcgcctt cagcgcctcg atcctctctc ccatgtcgag gatcccgtag acgggggcct
    12721 cggcgacgcc cgccatctcg gcgaggacgt aggtcacggg ctggaggttc ttgtggtaga
    12781 tgaaccgctc ggacttcgtc tccttcacgg tgacgagctc gcggagcggg acgaggcgcc
    12841 ccgtgcctcc gtggacggcc gtctggagga ggccgtcgag gctcgagcgc tgggaccggt
    12901 cgaggcggag gacgatcggg accggttttc gggagctctc gtcgcggagg agcccggcgt
    12961 cggcgccgtt cagcgcgacg cggagggtgc gcgtgacgac ctccggggtg actccggcgc
    13021 gggtcgcctt ctcgcggtcg acgacgagct ccaccttcgg ggcctcgtcc tccacgtgcc
    13081 agtcgacgtc gacgatgccg ggcgtctcct cgaagatcgt ccgcacctgg cgggcaaggt
    13141 cgatccgctt cgccatgtcg gggccgtaga tctcggcgac catcgtggag aggacgggcg
    13201 gcccgggcgg cacctcggtc accttgatgt tcgcgccgtg gcgcttcgcg atcggcagga
    13261 gcagcgtccg cacgtccttg gcgaacgtgt gcgagtcgcg cttccgctcc tgcttcggca
    13321 ggaggttcac ctgcaggtcg gccacgagcg ggccggagcg gaggaagtag tgccgcacga
    13381 ggccgttgaa gttgaacggc gcggtcgtcc cgacgtagac ctgcacgtcg gtcacctcgg
    13441 gctcgttccg caccttccgc gccaggtccc gcgcgacggc gttcgtcgcc tcgagcgtcg
    13501 tcccctcggg catgtcgacg acgacctgga tctcgctctt gttgtcgtac gggagcatct
    13561 tgaccttggc gaccttgaag tagaccagcg acatcgagag gaggaggagg acgacgactc
    13621 cgcccatgag cgcccagctc ttcgccgggc tcgtcaggag cggctcgaag agcctctggt
    13681 agaagcggtg gagcttgtcc tcctggggcg tcgcggcgtg gctctcgaag gcggagcggc
    13741 tcgcgtgctc ctcggcgtac ttccggaaga gcttgaacgt gagccagggg gagacgacga
    13801 acgcgacgaa gagcgagaag atcatcgccg ccgaggcgtt gatcgggatc ggggccatgt
    13861 acgggcccat gagcccggag acgaagacga gcggcaggag cgctccgacg acggcgaacg
    13921 tcgccaggat cgtcgggttc ccgacctcgt cgacggcgta gaccgtcgtc aggcgcggcg
    13981 gaccccagcc gagctcgtag tgccggtgga tgttctcgac gacgacgatc gcgtcgtcga
    14041 cgaggatgcc gatcgcgaag atgagggcga agagcgtgac gcggttcagc gtgtagccga
    14101 agaggtacga cgacgcgagc gtgagggcga gcgtcatcgg gacggcgacg aggacgacga
    14161 ccgcctcgcg ccgcccgagc gcgaacgcca tcaggaggac gacggagaac gtcgcgagcg
    14221 ccatgtggaa gatcagctcg ttcgacttct cgtcggcggt gtggccgtag tcgcgcgtct
    14281 tcgtgagcgc gacgttcgag gggacgacct tccccttcag gccggccacc ttcgcgtcga
    14341 gctcctcgac gagctcgacg gcgttcgtcc ccggcttctt ggcgacggag acggtgacgg
    14401 cggcggtatc ggctccggct ccgtgcgtcc ccccggctcc cgtcatatgc cagacgtatt
    14461 gggacggttc gtcggcgccg tcggtgacgg tggcgacgtc gcgcaggaag acggggcggc
    14521 cgttccagac ggcgacgacc gcgttgccga cctcctcggc ggagcgaaag agcgatccga
    14581 cctcgacctg ggtctcggcg tcggaggtcg cgaagctccc cgcggggagg cgccagttcg
    14641 cgccggagag ggccatgtag gcctgcagga gcgagacctg gtgggccgcg aggcggtcgc
    14701 ggtcgaagga gaccttcacg gcgcgcttct cgccgccgag gacggtcacc tgggcgacgc
    14761 gcgggtgctt cgtcagctcg accttcagct cctcggcgac ctccttgagc tgcatgggcg
    14821 aggcgtcggc ggagctgagc gtgtaggcga ggaccgggac gtcgtcgatc ccgcggggga
    14881 cgacgagcgg cggcatcgcg ccgggcggga gctcggaggc cttctcggcg agcttcgtgt
    14941 ggacccgtac cgccgcctgg tcggggtcgg tcccgacgag gaagcggacg atgatcatcc
    15001 cgcccgaggg ctgcgtggtc gagtagacgt actcgacgtt cgggatctcg tagatcgcct
    15061 tctcgagggg cgacgtcagc cgccgctcga cctcctgggc ggtggctccc ggcatcccga
    15121 ggaagacgtc gatcatcggg accttgatct gcggctcctc ctcgcgcggc gtcaccatga
    15181 cggcgaacgc gccgacgagg agggaggcga cgacgatgag cggcgtcagc ttcgactcga
    15241 ggaacgcctt cgcgatccgg ccggaggcgc cgacggggcg gcgggtcatc ttgaggcgga
    15301 gagcctcgcg cttcgtgccg gcgtgggacg gcggcggggt cggccccgcg gcgccctcgg
    15361 cggccttccc ggccgcctcg tcgcggggct cgctcactgg cccacctcgg aggaggcgtt
    15421 caccggggcg ccgtcggcgg gggcctgcgc gaggccgacg agtacgtcct cgcccccctt
    15481 caggccggag aggacctcga cgaggtcccc gtcgcgggtg ccgagcgtga cggcgcgggt
    15541 cctcgccttc ccctcctcgt ccttcacgac gacgaggctc atcgccccct ggcggaggac
    15601 ggcggcggcg gggacgacga cggccgtccg gcggcccgtc tcgaggagcg cccgaccggt
    15661 gacgccggtc ggcacctggg cccccgagac ctcgacgcgc gccgtgaagg agtggctcgc
    15721 ggggtcggcc ccggcggaca tctccacgac gcggccggcc agcgtcgcgc ccggcatcgc
    15781 gtcgagcgag acggggagga gcgcgccggt cttcagcttc gaggccacgg ccacgctctc
    15841 ggggaccgag aggacgaggc ggcggcccgc ggtcgactcg acgacgacga gcggccggcc
    15901 gggggcggcg aggtcgcccg ggtcgaccat cttccgggcc acgcgcccgg cgaacggcgc
    15961 gacgacgcgc gactcgcgcg cgacggagga ggcggcctcg acggcgccgg tcgcctgctc
    16021 gacggccccc ttcgcctgct cgtactgcat ccgggccagg tcgacctcga gctcggaggc
    16081 cgcggccgac ttctggagcg cctggaacct ctgccagttc cgctccgcga gggcgagggc
    16141 cgccttcgcc tgcgcgagcg cgccgcgagc ctgcgactcc tgccccttcg ccgtctgagg
    16201 gtcgatctcg acgaggagct ggccctgggc cacgaggtcg ccttccttca ccgggacggc
    16261 gacgacggcg gccatcacgc ggctgctcac catcgcggac ttgccggctt cgacggtgcc
    16321 ggagagctcg atccgctgcg gcagctcgcg ggactcggcc ttcgcgaggg cggcggtgac
    16381 gggggcgggg gcggcggcct cgccggcctt cttcccgcct ccgcaggcgg agagggcgag
    16441 cgtgcccgcc acggtgatcg cgacgaggga gagggtcttc cgaggggtca tgcgagcctc
    16501 cgtgctcttc ttcatctggt cgggggtgcg ggggcgggct tccgtcacgg gatcgccgtc
    16561 tccggcgcct tccccgcctt gacggcgagg cggagcgcgg tggcgtgcgc gtcggcccgg
    16621 gcgacgagct cgcgggtctc ggcctccctt cgggcggtcg ccgcgtcgag gaggtcgatc
    16681 gtcttcacga cgccgctccg gaagcgctcg tcggtgatcc gctcgctctc gcgggcggcc
    16741 tcgagcgcct tcacggccgt cgcgtggcgg gcgcgggccg tcgcggcctc ctcgtacgcc
    16801 tgcctcacct cgagggcgac gccgtccgcg aagcgggcga cgtcctcgcg cccggcgcgg
    16861 gcctcctcgc gcgcggcgac gagggcggcc ttgtcggcgc ccccctggaa gaggttcacc
    16921 gaggcgaagg ccatgaccgt gcccgccgag ccgctcgcgc cgaagaggtt cctgtcgtgg
    16981 aggtcccacc gggccatcac gcccaccttc gggagccagg cggccttctt gaccttctcc
    17041 tcgagctcgc cggccgcaag caggctccgc gcggccgaga ggtccctgcg ggagccggcg
    17101 gaggagaccc aggaagcggc gtcgccgtcg aggggctttg ggccggggag gggcgcgaga
    17161 tcccaggtgg agtcctgggc gacgccgatc cggaacgcca ggttcgcgtt cgcgacccgg
    17221 gccttcccct tcgcgtcctg gagcatgtcc tcgacccgcg cgagctccac ctcggcccgg
    17281 aggagctcgg atcggacgag catcccctgc tcgacgtagc tct
//
LOCUS       DAOONL010000072        17218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3452_length_17218_cov_15.543145, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000072 DAOONL010000000
VERSION     DAOONL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3452_length_17218_cov_15.543145"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(737..1399)
                     /gene="purN"
                     /locus_tag="PLP50_11265"
     CDS             complement(737..1399)
                     /gene="purN"
                     /locus_tag="PLP50_11265"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPA52170.1"
                     /translation="MGGSFRAPVPWFPLPARLAVLLSGRGSNFEALADACDSGALPAR
                     IVLVLSDRADAAGLEKARARGIAAAVEERGGEPRAAHEARLAGRIRSAGTDVVCLAGF
                     MRVLSPAFVERFERRIVNVHPSLLPAFPGLEAQRQAFEHGVKVAGATVHLVDAGTDTG
                     PIAGQEAVPVRPGEDAANLAARILEAEHRLYPATLAALLDGGWVIEGRCLRFPAPGNT
                     EA"
     gene            complement(1405..2445)
                     /gene="purM"
                     /locus_tag="PLP50_11270"
     CDS             complement(1405..2445)
                     /gene="purM"
                     /locus_tag="PLP50_11270"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPA52171.1"
                     /translation="MPNDTPLTYAASGVDIDAKMSAVARAKEAIRSTFTPGVVGDVGG
                     FGGLFRPDFRGMADPLLVASADGVGTKIRVAIAADVHGTVGQDIVNHCVNDILVQGAR
                     PLFFLDYVATGRMRRDVIAEVIGGVATACRENGCALLGGETAEMPGMYADGDYDLAGT
                     IVGVVDRPKLLDGSRVAEGDLLLGLPSSGLHTNGYSLARMLFFERLGLGPHDAVPELG
                     TTVAEALLAVHRSYLATLLPLVDEGLLSAISHVTGGGFPDNLPRVLPEGLRAVVTPEA
                     WEWPALFRFIREKGSVTQEEMLRVFNCGVGMVLIVAPERAPEVRWRLEGAGETVLDVG
                     RVENGPRGVRFA"
     gene            complement(2504..3961)
                     /gene="purF"
                     /locus_tag="PLP50_11275"
     CDS             complement(2504..3961)
                     /gene="purF"
                     /locus_tag="PLP50_11275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPA52172.1"
                     /translation="MSGETESIETDGFREECGVFGIYGHPDAANLAYLGLYALQHRGQ
                     EAGGIAAWNGRRIAVEKGMGYVADLFDEARLARLPGKSAVGHVRYSTAGASLLENAQP
                     IVYNTNKGPLAIAHNGNLVNADELRAELEQEGSIFTTSSDTEVFLHLMARSRAESLVD
                     ALRDALGRARGAYSIVLLAGEKVLAARDPQGFRPLTIGRLGDAWVVASETCAFDLIDA
                     EPVRDVEPGEIVILDAGGVASASFAMGRRTAFCVFEHVYFARPDSNVFSRSVAESRRA
                     FGRRLAREHAVAADVVVPVPDSGIFAALGYAEESGIPYGMGLVRNHYVGRTFIEPKQS
                     IRSFGVKVKLNPVKSVVGGKRVVLVDDSIVRGTTSKKLVRLLKSAGATEVHMRISSPP
                     TTHPCHYGIDTPRRKELIAATHEMEEIRRFVEADSLGYLSLEGMLGAFGKGEGEACAA
                     CFSGRYPVPFAAALEQQPLFGEDEVLPASGSPEKR"
     gene            complement(3984..4403)
                     /locus_tag="PLP50_11280"
     CDS             complement(3984..4403)
                     /locus_tag="PLP50_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPA52173.1"
                     /translation="MARPDPHPPEVFDRLRRRGPETRIAVVGASNHPEKYGNVIVRNL
                     AAKGYTVLPVNPRETEIAGLPAFPDLESVPGPVHIVNVVVPPRVTLGVLEKAGELGIP
                     AVWLQDGSFDDAVLEAAARAPFETVYDACIMVASSFG"
     gene            4581..5057
                     /locus_tag="PLP50_11285"
     CDS             4581..5057
                     /locus_tag="PLP50_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52174.1"
                     /translation="MKGTPSSGLWVLLLLVAAGAAVFWLARRGFKADDAIVREADRTV
                     VAEYVRLVGERKYAEAWERCLTKSYREEVPREKFVAAHEKRRAETGALAGAKLLRQQV
                     SRNLFTRTRTLHLLYELSYPGRTVPEYAVLNDADGAFLIEGTYHETAGETLDFLLW"
     gene            complement(5067..6173)
                     /locus_tag="PLP50_11290"
     CDS             complement(5067..6173)
                     /locus_tag="PLP50_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPA52175.1"
                     /translation="MSAAKKRFLLYGANGYTGRLILDAALEKGLLVTVAGRRREAIEP
                     LAKERGLPFLVFGLDEAPRRLAERVAPFGALLLAAGPFSRTSAPALAACLAARVPYLD
                     ITGEIAVLEAVFARHPEAVRAGVPLLPGVGFDVVPSDCLAAALAAALPGAVRLELAFR
                     GFGLSGGTARTMLEGLPKGALARVDGKLVAVPPAWKTRTVPFPDRTRLAVTIPWGDLS
                     TAYRSTGIPNIETYVAMPPVRVAALRWAGPLLPLAGLLPLQRVVGPWVRNVVTGPSAE
                     ERARERSLLWGRVEDGEGRSVEGTVETLEGYTLTAETATLAVERVLRRAVEPGAFTPS
                     KAFGARCIEKVRDTKMNVPARATGAAARKAARSE"
     gene            6213..6986
                     /locus_tag="PLP50_11295"
     CDS             6213..6986
                     /locus_tag="PLP50_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA52176.1"
                     /translation="MTSDAWDPSRYAKFGAERAAPFFDLLDLVEPRPGMRVLDLGCGT
                     GELTRALHERLRARETLGVDRSAKMLGKSATFVAPGLRFAEGTIESYAADAPFDLVFS
                     NAALHFVADHESLWPRLAALLAPGGQLAVQLPANQDHPTHGAAREVAAGPPFDAALGG
                     WVHPTHVLPPEAYAVLLHRLGFARQTVRLAVYTHLLESPESAVEWVRGSILTEYESRL
                     GPELFPEFLASYRARLLPRLGEERPYFFTFKRVLIHGAL"
     gene            7090..9198
                     /locus_tag="PLP50_11300"
     CDS             7090..9198
                     /locus_tag="PLP50_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52177.1"
                     /translation="MTMPFRSARAARRLASALVLALLLPAPRGLAEEVDGPAGPRVSI
                     PLADYESLRKLREAPSLTVVDTLRVSGSFASRTLALSLSGRSSGTLPAATLLESIEGA
                     ALAGCDGSALVSRGTEAAYTLTPLGARFEVRCRLVPSSGDRLSFVAGRAVLFPEGDVA
                     DGEFLPGPDPGDGTRPFTVVRRIAADGEALAPTATGRYRITLLPDETRFRFTIEVRNP
                     NRGRAPFDLVLGSGERVQEVVAPGPYDVKEGRTRFDLPPGDGTIVLTGTLGGTEFHAP
                     VDAALSYLLLESHPLLRAEARTAAKRVSPQETGMPREFRGGQAFLLGKGDAFAWSVKR
                     LEALRSTSYAVRATAQTLFVPADGPVLGQSLFELDNQGASDLALPSSPEPTYAAFGDE
                     AVFLTRNEKGELWLPLASGPQALLLQSRQGLGRSLGFAAGSLAVPHLPVPSTRYAVEL
                     RYPDAWVPLYERFGGESRLAVPSIEACVVFLLLSGWLFGLLGALRFEPRRAALLAGLL
                     ALSAFASDTAAYVVVAAAIVLTVVWIAAVARERRPRLRTLVALGALGAFAALVVLAVA
                     IPSGLLRSRGPEAPPAEVAFYSESASAPVARKAVRSDAPASPAPEPQAAGGPSYEGLP
                     AKVELPRGARSTWWSREMLAADAAPVVRVVLAGRTLVSLAQGLAVLSALGLLWWSRPS
                     LREGLRALRARGRQEVPAGR"
     gene            9311..10561
                     /gene="ispG"
                     /locus_tag="PLP50_11305"
                     /gene_synonym="gcpE"
     CDS             9311..10561
                     /gene="ispG"
                     /locus_tag="PLP50_11305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPA52178.1"
                     /translation="MTTNAKRHATVAVKVGKVTVGGGAPVVVQSMTSTDTADVEATTK
                     QCLELAEAGSELVRVTVNLPEAAAAVPEIRRRLDDAGCDVPLIGDFHYNGHTLLTSYP
                     EMAKALAKYRINPGNVGTGRRRDEQFATICAVARDNAKAVRIGVNGGSLNQELVLAKM
                     QENTDRDLGRTPEEILNECMVLSALQSTELALENGLRKDQIVISCKVSRPPELISLYR
                     DLASKTDQPLHLGLTEAGMGTKGLVWSSAAMGVLLAEGIGDTIRVSLTPRPGGDRREE
                     VYAACELLQSLGLRAFAPSVTACPGCGRTTSTTFQELAERIEGYVREKMPLWKAEREG
                     VETMTLAVMGCIVNGPGESKAASIGISLPGTGEAPSCPVYVDGQKFTTLKGSYEELAA
                     AFQALVDDYVATKYPLRSGSAREA"
     gene            10608..11192
                     /locus_tag="PLP50_11310"
     CDS             10608..11192
                     /locus_tag="PLP50_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52179.1"
                     /translation="MNAETPDRLDPRAMVKGTMLRAHLSWATNRFGAGYEPVRQRLSE
                     PVVALLSRPVLPTDWIPVSSLIELDRAIADAAGEPPETTWLALGRHSAALNLTGVYKS
                     FISSEPHRFFERMTVLHHQFQSFGKSVYERTGERSGRIRIESPSTYSRVYCVSGKGYY
                     EEALRLLQAPGPIVVRETECATAGAAACVFELAW"
     gene            11189..13585
                     /locus_tag="PLP50_11315"
     CDS             11189..13585
                     /locus_tag="PLP50_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA52180.1"
                     /translation="MSPEVRGEDSPAADLTLFEQVARAIDDFVLVVDLQGRLTHVNDA
                     AVERSGWLREELLGKPWTVFLPPAERDELAARIADETRAGGFRGDVKNVTKSGKALWV
                     ALRTSVLRRDGKAVGFVSISRDIGEQKRVEAELTAARDAAEAASRAKSEFLANISHEI
                     RTPMNAVIGMATLLLDTALTPEQRDYVETIRTSGDTLLALLNDVLDFSKMESDRLELE
                     ARPFSFREAVEDSFDLVAPSAASKGLDLAYRVDPSVPASLLGDVTRVRQVLLNLLTNA
                     VKFTSSGEVLLRASARPAAEGSHRVEIVVRDTGIGIPKERLDRLFRSFSQVDPSTTRQ
                     YGGTGLGLAICKRLAEKMGGGIEAESSGRGSTFRVALVVPDGPPRRDAPVPGIDRLAG
                     QRVLIVDDGPASRDTLFETLSGWGMQPRATSSPREALAWVGAGEPFDLGLIDEEMAEV
                     DGRRFAESLRRFRDADALPLVLVTSRGRRAGGARAPFAAVVARPVRPRALASVLAGLR
                     DDSVSRRRRRSSPALPKELGVSHPLRVLLAEDNAVNQKVALSYLARMGYRADLARTGI
                     EALEAVRRNVYDVVLMDVQMPEMDGLEAARRIRSFAPAENGPWIIALTASAMTGDAER
                     CLAAGMNDYLAKPVRGEDLAAALRRAPRSASATGPASALTAGRLVLLPGPLAKLRELE
                     RPGETGFLASLLEIFRRDTPIRLEEMERAAATADAEALRRAAHTLKSSAATLGGARVE
                     TLAARTEVLAMNPTTLAEAAALIPALRSESAALLAALGLAEGEEGARLSLDPGDDRRS
                     "
     gene            13681..14799
                     /locus_tag="PLP50_11320"
     CDS             13681..14799
                     /locus_tag="PLP50_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPA52181.1"
                     /translation="MSAYKIAWLPGDGVGNEVMEAARITLDALKLDASYTHGDIGWEF
                     WRTEGESFPQRTIDLLSSSDAAMFGAITSKPVKAAEQELAPELQGKGLTYRSPIVRMR
                     QMFDLYVCLRPCKAYPGNPLNFKEAIDLTIFRENTEDLYAGVEFSPVPDALATTLAAL
                     SKPFGHFADLPSDQYAVSCKVNTRKGSERIARAAFEYARKFGRKKVTIVHKANVVRAT
                     DGLFFEEAKKVAADFPEIAVDDANVDAICMWLLKNPMSYDVLVAPNLYGDIISDLAAQ
                     MVGGLGFGCSGNIGKKLAVFEPSHGSAPKYAGQYKVNPLATILAAKMMLDFLGEPEKG
                     AKLEAAVAKVIAEGKVRTYDMGGSATTLEMGEAVARAL"
     gene            14783..15739
                     /locus_tag="PLP50_11325"
     CDS             14783..15739
                     /locus_tag="PLP50_11325"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPA52182.1"
                     /translation="MPGRSDVAADRIVLHEVGPRDGLQAEKTVVPTETKLRWIGALCE
                     AGLDVVQVGSFVHPEKVPQMADTDALFRALTAPGAERKGHAIYSGLVLNEKGLDRALA
                     CGVEMVCLGVSASETHSRKNTGMGTAEATERIVATARKAVEAGRKVQVSVQSAFGCGF
                     EGHVPEERVIDIARAYVDAGLVTIALADTAGHAYPEQVERLYGKLLALAPGIEAACHL
                     HDTYGAGMANVYAALRAGAVSLESSFAGLGGCPFTKVAAGNVATEDLVHTLQRDGKRT
                     DVDLEGLIAVAKDVEAFLGREMPGRVHRTGPVPQLRKQEASV"
     gene            15736..16935
                     /locus_tag="PLP50_11330"
     CDS             15736..16935
                     /locus_tag="PLP50_11330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPA52183.1"
                     /translation="MTRKALEGVVVLDLTNVLSGPFATLHLALLGAEVIKVENPTDGD
                     LARKLGNVPKLNKELMGTSFLAQNANKKSLTLNLKKPEAKEIFKKLAATADVVVENFR
                     PGVMRRLGVGWETLRVINPRLVYCAISGFGQDGPDADKPAYDQIIQGLSGEMDVNGDE
                     RLHPLRAGFPVCDTVGGMNAAFAILAALYHRERTGEGQAIDVALLDSIMPLMGWVAAN
                     LLIGGAPPVAIGNDNFTAAPSGMFRTKDGHINIAANKQEQWESVCDVLGVPELKADPR
                     FAERDTRKKNRHALTPLLEVKLAERPTSEWVDLLNAKDVPSGDILSLEAALKQPQVVH
                     RKTLASVETPGVGTLPLFNLTAKLEKTPGVIDAPPPRLGAHTAEILGRIGYSTDDVAR
                     LSAEGVV"
     gene            16946..>17218
                     /locus_tag="PLP50_11335"
     CDS             16946..>17218
                     /locus_tag="PLP50_11335"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPA52184.1"
                     /translation="MGKTVVEKILARAAGLPEVTAGDVVEPRVDVAMSHENAALVINQ
                     FLEIYEGTGVTPHPWDVSKIAIIFDHRVPAESPKTASNQKKIREFVD"
BASE COUNT         2495 a         6261 c         6120 g         2342 t
ORIGIN      
        1 gcatgtgtta ggcacgccgc cagcgttcac tctgagccag gatcaaactc tccaaaaaaa
       61 gtttgaaact tgtctcaacg ctcggcccga aggcctcgcg cagaagtgcg ttttactgaa
      121 ggccccgacg ccagcggcgc cgaagcccgg gctcgtcccg ccggacaagc ccctcaaagg
      181 aatcacgtaa acccccatga ggactggact tacgcccacc ctcacgggtt cgtctctgtt
      241 cagttttcaa ggaaccggtg ccgtccagcg gcggattccc agtctctcgc aggcagcctt
      301 tgaagtcaag agcttttttc gggggccgcc cggaggcggg ctcctgtcag aaagcgctct
      361 cggattttct cgggttttgc tcgctggaaa gatcgggccc gggcatcgcc gggggagggg
      421 aagatacgga agttgccccg ggatgtcaag cgcaaacctt cggcgagcga aaaaaggcgc
      481 gccggagatc ggacggtcac tccctcgtaa gggaggacac cgggtgaggg cgacccggcg
      541 tccaagaccg agaacccgct ccggcgcgag gagagagcgt cgtcccatgc gccgaccgaa
      601 acctaacccg ctatgggtca ataggttccc accgccgcac ctttccgtct gtttgcttga
      661 gagggaggct aagcctgtcg atcgggcgtc gcaatcccac taaagtggga tttcgtctcg
      721 aagaacgtgc ctacggtcac gcttcggtgt tcccgggggc cggaaaccgg aggcagcgcc
      781 cctcgatcac ccagcccccg tccagaaggg ccgcgagggt ggccgggtag aggcggtgct
      841 cggcttcgag gatgcgggcc gccaggttcg ccgcgtcttc gcccggccgg acgggaacag
      901 cctcctggcc ggcgatcggg ccggtgtcgg tgcccgcgtc gacgaggtgg acggtcgcgc
      961 ccgcgacctt cacgccgtgc tcgaaggcct gacgctgcgc ctcgaggccc ggaaacgcgg
     1021 ggagaaggga ggggtgaacg ttgacgatcc gccgctcgaa ccgctcgacg aaggccggtg
     1081 agaggacgcg catgaagccc gcgaggcaga cgacgtcggt cccggcggag cgtatgcgcc
     1141 cggcgaggcg tgcttcgtgc gcggcgcgcg gctcgccgcc ccgctcctcg accgcggccg
     1201 cgatcccccg ggcgcgcgcc ttctcgagcc ccgcggcgtc ggcccggtcg gagaggacga
     1261 ggacgatccg cgccggcagc gcgccggagt cgcaggcgtc cgcgagcgcc tcgaagttcg
     1321 agccgcgccc cgagaggagg acggcgaggc gcgccgggag cgggaaccag ggaacgggcg
     1381 ctctgaagga gccgcccacg gccgtcatgc gaagcggacg ccgcgcgggc cgttctcgac
     1441 gcgcccgacg tcgaggaccg tctcccccgc accctcgagc cgccacctca cctccggcgc
     1501 gcgttccggc gcgacgatga ggaccatccc gacgccgcag ttgaagacgc ggagcatctc
     1561 ctcctgcgtc acgctcccct tctcgcggat gaaacggaaa agggcgggcc actcccacgc
     1621 ctccggcgtg acgacggccc ggagcccctc gggcaggacg cgcgggaggt tgtcagggaa
     1681 gccgccgccg gtgacgtggg aaatcgccga gaggagcccc tcgtcgacga gtggaaggag
     1741 cgtggcgagg tagctgcgat ggacggcgag gagcgcctcc gcgaccgtcg tcccgagctc
     1801 gggaacggcg tcgtgcggac cgaggccgag gcgctcgaag aagagcatcc gcgccaggga
     1861 gtagccgttc gtgtggaggc ccgaggaggg gagcccgagg aggaggtctc cctcggcgac
     1921 gcgcgagccg tcgaggagct tcggccggtc gacgacgccg acgatcgtcc cggcgaggtc
     1981 gtaatcgccg tcggcgtaca tccccggcat ctcggcggtc tcgcccccga ggagcgcgca
     2041 gccgttctca cggcaggccg tggcgacgcc cccgatgacc tccgcgatca cgtcacgccg
     2101 catccgcccc gtcgccacgt agtcgaggaa gaagagcggg cgcgccccct ggacgaggat
     2161 gtcgttcacg cagtggttga cgatgtcctg cccgaccgtg ccgtggacgt cggccgcgat
     2221 ggcgacccgg atcttcgtgc cgacgccgtc cgcggaggcg acgaggagcg ggtccgccat
     2281 cccgcggaag tcgggccgga agaggccgcc gaacccgccg acgtcgccga cgacgccggg
     2341 ggtgaaggtg gaacggatcg cctctttcgc gcgggcgacg gcggacatct tcgcgtcgat
     2401 gtcgacgccg gaggcggcgt aggtgagcgg cgtgtcgttc ggaatggcgc gcctcctcgg
     2461 aggggatcgg cccgtcggac gtccctcggg cggcgcgatg gtatcagcgc ttctcggggc
     2521 ttcccgaggc ggggaggacc tcgtcctccc cgaagagcgg ctgctgctcg agggcggccg
     2581 cgaaggggac ggggtagcgc ccggagaagc aggcggcgca ggcctcgccc tccccctttc
     2641 cgaaggcgcc gagcatcccc tcgagcgaga ggtagccgag ggagtcggcc tcgacgaagc
     2701 gccggatctc ctccatctcg tgggtcgcgg cgatgagctc cttgcggcga ggggtgtcga
     2761 tcccgtagtg gcagggatgg gtcgtcgggg gggaggagat ccgcatgtgg acctcggtcg
     2821 ccccggccga cttcaggagc cgcacgagct tcttcgacgt cgtcccgcgg acgatcgagt
     2881 cgtcgacgag gacgacgcgc ttcccgccga cgaccgactt caccgggttc agcttcacct
     2941 tcaccccgaa gctccggatc gactgcttcg gctcgatgaa ggtccggccg acgtagtggt
     3001 tccggacgag ccccattccg taggggatgc cgctttcctc ggcgtagccg agcgccgcga
     3061 agatgcccga gtcggggacg gggacgacga cgtcggcggc gaccgcgtgc tcgcgggcga
     3121 ggcgccggcc gaatgcgcgg cgagactcgg cgacggagcg actgaagacg ttggagtcgg
     3181 gacgggcgaa gtagacgtgc tcgaagacgc agaacgcggt ccggcggccc atcgcgaacg
     3241 aggccgaggc gacgccgccg gcgtcgagga tgacgatctc cccgggctcg acgtcgcgca
     3301 ccggctcggc gtcgatcagg tcgaaggcgc acgtctcgga ggcgacgacc cacgcgtcgc
     3361 cgaggcgccc gatcgtgagc gggcggaagc cctgcgggtc gcgcgcggcg aggactttct
     3421 ccccggccag gaggacgatc gagtaggcgc cgcgggcgcg accgagggcg tcgcgaaggg
     3481 cgtcgacgag gctctcggcc cgcgaccggg ccatcaggtg gaggaagacc tccgtgtcgg
     3541 aggaggtcgt gaagatcgac ccctcctgct cgagctccgc gcggagctcg tcggcgttca
     3601 cgaggttccc gttgtgcgcg atcgcgagcg ggcccttgtt cgtgttgtag acgatcggct
     3661 gggcgttctc gaggagcgag gctcccgccg tcgagtagcg gacgtgcccg accgcggact
     3721 tccccggcag gcgcgccagc cgcgcctcgt cgaagaggtc ggcgacgtag cccatcccct
     3781 tctcgacggc gatccggcgg ccgttccagg ccgcgatgcc gcccgcctcc tggccgcggt
     3841 gctggagggc gtagaggccg aggtaggcga ggttcgcggc gtcgggatgc ccgtagatcc
     3901 cgaagacgcc gcactcctcc cggaacccgt ccgtttcgat cgactcggtc tctccgctca
     3961 cggcgcgaag tgtatcgccc gggtcagccg aagctgctcg ccaccatgat gcaggcgtcg
     4021 tagacggtct cgaacggcgc tctcgcggcc gcctcgagga ccgcgtcgtc gaagctcccg
     4081 tcctggagcc agacggccgg aatgccgagc tcccccgcct tctcgaggac cccgagcgtc
     4141 acccgcggcg ggacgacgac gttcacgatg tggacgggac ccgggaccga ttcgaggtcc
     4201 gggaaggcgg ggagccccgc gatctcggtc tcgcggggat tgaccgggag gacggtgtac
     4261 cccttcgcgg cgaggttccg gacgatgacg ttcccgtact tctccgggtg gttgctcgcg
     4321 ccgacgacgg cgatccgcgt ctcgggcccg cgccggcgga gccggtcgaa gacctcgggc
     4381 gggtggggat cgggacgggc catcggagac ctcctcgccg ggcgctcggg ccgggcggcc
     4441 gattctgcga cgaccccggc ccgcccgcga aggtccgccc ggcgtacgat ggccggtggc
     4501 cctttcgtgc gtggaacgcg cgcgagggcg cccggcgccg acgggacgat ccggaacgac
     4561 ggggccggcc gggaggagcg atgaagggaa cgccgtcgtc cggcctctgg gtcctcctgc
     4621 tcctcgtcgc cgccggcgcg gcggtcttct ggctcgcgcg acgcggcttc aaggccgacg
     4681 acgcgatcgt ccgggaggcc gaccggaccg tcgtcgccga gtacgttcgc ctcgtcggcg
     4741 agcggaaata cgccgaggcg tgggagcggt gcctcacgaa gagctaccgc gaggaggtcc
     4801 cccgcgagaa gttcgtcgcg gcccacgaga agcggcgggc cgagaccggg gcgctcgcag
     4861 gcgcgaagct gctgcgccag caggtgagcc ggaacctctt cacgcggacc cgcacgctcc
     4921 acctcctcta cgagctctcg tacccgggta ggaccgtgcc cgagtacgcc gtcctgaacg
     4981 acgccgacgg cgcgttcctc atcgagggga cctaccacga gacggcgggc gagacgctcg
     5041 acttcctcct ctggtaggac ggggcgtcac tccgaccgcg cggcctttcg ggccgccgcg
     5101 ccggtggccc gggcggggac gttcatcttc gtgtcgcgga ccttctcgat gcaccgggcc
     5161 ccgaacgcct tcgagggtgt gaaggctccc ggctccaccg cgcggcgcag gaccctctcc
     5221 acggcgaggg tggcggtctc ggccgtcagc gtgtagccct cgagcgtctc gacggtccct
     5281 tcgaccgagc gcccctcccc gtcctcgacg cgcccccaga ggagcgaccg ctcccgggcc
     5341 cgctcttcgg cggacggtcc ggtgacgacg ttcctcaccc aggggccgac gacgcgctga
     5401 agaggcagga gcccggcgag ggggagaaga ggacccgccc agcgcagggc ggcgacccgg
     5461 accgggggca tggcgacgta ggtttcgatg ttcgggatgc ccgtcgaccg gtaggcggtc
     5521 gagaggtcgc cccacgggat cgtcaccgcg agccgcgtcc ggtcggggaa cgggacggtc
     5581 cgcgtcttcc aggccggcgg caccgcgacg agcttcccgt ccacgcgcgc cagagctccc
     5641 ttcggcagcc cctcgagcat cgtccgggcc gtgccgcccg agagcccgaa gccgcggaac
     5701 gcgagctcga gccggacggc gccggggagc gccgccgcga gcgcggcggc gagacagtcg
     5761 gacgggacga cgtcgaagcc gacgcccggc aggagcggga cccccgcgcg gacggcctcc
     5821 gggtgacgcg cgaagaccgc ctcgaggacg gcgatctccc ccgtgatgtc gaggtagggg
     5881 acgcgggccg cgaggcacgc cgcgagggcc ggcgcgctcg tccgggagaa cggcccggcc
     5941 gcgaggagga gcgccccgaa cggcgcgacc cgctccgcga ggcgccgcgg cgcctcgtcg
     6001 agcccgaaga cgaggaacgg gaggccgcgc tccttcgcga gcggctcgat cgcctcgcgc
     6061 cggcggcccg cgacggtgac gaggagaccc ttctcgagcg cggcgtccag gatcagccgt
     6121 cccgtgtatc cgttggcccc atagaggagg aagcgcttct tggcggcaga cacgaccggt
     6181 cccatcgtac gccgtccgct acgatcgcgc ccgtgacgag cgacgcgtgg gacccttcgc
     6241 gctacgcgaa gttcggggcg gaacgggccg cgcccttctt cgacctgctc gacctcgtcg
     6301 agccgcggcc cgggatgcgc gtcctcgatc tcggctgcgg gacgggagag ctgacgcggg
     6361 ccctccacga acgtctccgc gcgcgggaga cgctcggagt ggaccggtcc gcgaagatgc
     6421 tcgggaagag cgccacgttc gtcgcgccgg gcctccgctt cgcggagggg acgatcgagt
     6481 cgtacgccgc cgacgccccg ttcgacctcg tcttctcgaa cgccgcgctc cacttcgtcg
     6541 cggaccacga gtcgctctgg ccccgcctcg ccgccctcct cgccccgggg ggacagctcg
     6601 cggtccagct cccggcgaac caggaccacc cgacgcacgg tgcggcccgg gaggtggctg
     6661 ccgggccgcc gttcgacgcg gccctcggcg ggtgggttca cccgacgcac gtcctcccgc
     6721 ccgaggcgta tgcggtcctg ctccatcggc tcgggttcgc gaggcagacg gtccggctcg
     6781 ccgtctacac gcacctcctc gagtctcccg agagcgccgt cgagtgggtc cggggctcga
     6841 tcctgaccga gtacgagtcg cgcctgggcc cggagctctt cccggagttc ctcgcctcgt
     6901 accgcgcccg cctcctcccg cggctcggcg aggagcggcc gtacttcttc acgttcaagc
     6961 gcgtcctgat ccacggggcc ctctgaccgg cgcggcccgg gccgccggga gccgtcccgt
     7021 gacgccgggc acccgtccga cccctgccgg gaaccgggac gggcgagtag agtcgaagct
     7081 cccgaggtga tgacgatgcc gttccgctcc gcgcgcgccg cccgaaggct cgcttccgcg
     7141 ctcgtcctgg ccctcctcct cccggccccg cggggcctcg ccgaggaggt cgacggtccg
     7201 gccggaccgc gggtctcgat ccctctcgcg gactacgagt ccctccgcaa gctgcgcgag
     7261 gcgccgagcc tcacggtcgt cgacacgctg agggtctccg ggagcttcgc gtcgcggacg
     7321 ctcgcgctct ccctctcggg tcgctcctcc gggaccctgc ctgccgccac actcctcgag
     7381 tcgatcgagg gggccgcgct cgcggggtgc gacgggagcg ccctcgtctc gcgcggcacc
     7441 gaggcggcct acacgctgac gccgctcggc gcgcgcttcg aggtccgttg ccggctcgtc
     7501 ccctcttcgg gagaccgtct ctcgttcgtc gccggccggg ccgtcctctt cccggagggg
     7561 gacgtcgcgg acggcgagtt ccttcccggc cccgacccgg gcgacgggac gaggcccttc
     7621 accgtcgtgc ggcggatcgc cgcggacggc gaagccctcg cgccgacggc gacgggacgc
     7681 tatcggatca cgctcctccc cgacgagacc cgcttccgct tcacgatcga ggtgaggaac
     7741 ccgaaccggg gccgcgcccc gttcgacctc gtcctcggca gcggcgagcg ggtccaggag
     7801 gtcgtcgcgc ccggcccgta cgacgtgaag gaagggagga cgcgcttcga cctgccgccc
     7861 ggcgacggga cgatcgtcct gacggggacc ctcggcggga cggagttcca cgcgccggtc
     7921 gacgcggcgc tctcctacct cctcctcgag agccacccgc tgctccgggc cgaggcgcgg
     7981 accgcggcga aacgggtcag cccgcaggag acggggatgc cgcgcgagtt ccggggcggg
     8041 caggcgttcc tcctcgggaa gggcgacgcc ttcgcctgga gcgtgaagcg cctcgaggcg
     8101 ctccgctcga cgagctacgc cgttcgcgcg acggctcaga ccctcttcgt cccggccgac
     8161 ggccccgtcc tcgggcagtc cctcttcgag ctcgacaacc agggagcctc cgacctggcg
     8221 ctcccgtcct ccccggagcc gacttacgcg gcgttcggcg acgaggcggt cttcctgacg
     8281 aggaacgaga aaggggagct ctggctcccg ctcgcctccg ggccgcaggc gctcctcctc
     8341 cagagccgac agggcctcgg ccggtcgctc ggtttcgcgg ccgggagcct cgcggtaccg
     8401 cacctgccgg tcccctcgac gcggtacgcc gtcgagctcc gctaccccga cgcctgggtc
     8461 cccctctacg agcgcttcgg cggagagagc cgcttggcgg tgccgtcgat cgaagcgtgc
     8521 gtcgtcttcc tgctcctctc ggggtggctc ttcggcctcc tcggcgcgct ccggttcgag
     8581 ccgcggcgcg cggccctgct ggccgggctc ctcgccctct cggcgttcgc ctcggacacc
     8641 gccgcgtacg tcgtcgtcgc cgcggcgatc gtcctgacgg tcgtctggat tgccgccgtc
     8701 gcgagggaga gaaggccacg cctccgcacc ctcgtcgcgc tcggcgcgct cggcgcgttc
     8761 gccgcgctcg tcgtcctcgc ggtcgcgatc ccctccggcc tcctccgctc gcgcggcccc
     8821 gaggcccctc ccgccgaggt cgccttctac tccgagtccg cgtcggcgcc ggtggcccgc
     8881 aaggcggtgc gctccgacgc gcccgcgtcc cccgcgccgg agccgcaggc ggcgggcggt
     8941 ccgtcctacg aggggctccc cgcgaaggtc gagctgcccc gcggcgcccg aagcacgtgg
     9001 tggagccgcg agatgctcgc ggccgacgcc gccccggtgg tccgcgtcgt cctcgccggc
     9061 cggacgctcg tctcgctcgc gcagggtctc gccgtcctct ccgccctcgg cctcctctgg
     9121 tggtcgcgtc cgtcgctgcg cgaggggctc cgggcgctcc gggcccgggg acggcaggag
     9181 gttccggcgg gccgctgacg ccccccggcg ccgcctcggc tccgccgccc ggcgggccgg
     9241 cggcggagag gggccggtcg gctttgctaa cgtggcgcgc gcgcgggacg cgtcccgcgg
     9301 gaggtcctcc ttgacgacga acgcgaagcg ccacgccacg gtggcggtga aggtcgggaa
     9361 ggtgacggtg ggcgggggcg cgcccgtcgt cgtccagtcg atgacgtcga ccgacacggc
     9421 cgacgtcgag gcgacgacga agcagtgcct ggagctcgcc gaggcgggct cggagctcgt
     9481 ccgcgtcacc gtgaacctcc ccgaggcggc ggcggcggtc cccgagatcc gcaggcgcct
     9541 cgacgacgcg ggctgcgatg tccctctcat cggcgacttc cactacaacg gccacacgct
     9601 cctgacgtcg tatccggaga tggcgaaggc cctcgcgaag taccggatca accccggcaa
     9661 cgtcgggacg ggacggaggc gcgacgagca gttcgccacg atctgcgccg tggcccgcga
     9721 caacgcgaag gcggtgcgca tcggcgtgaa cggcggctcc ctgaaccagg agctcgtcct
     9781 cgcgaagatg caggagaaca ccgaccgcga cctggggagg acgccggaag agatcctgaa
     9841 cgagtgcatg gtcctctctg ccctccagtc gacggagctc gcgctcgaga acggcctccg
     9901 gaaggaccag atcgtcatct cgtgcaaggt gtcccgcccg ccggagctga tctcgctcta
     9961 tcgcgacctc gcctcgaaga ccgaccagcc gctccacctc ggcctgacgg aagccgggat
    10021 gggaacgaag gggctcgtct ggtcgtcggc ggccatggga gtcctcctcg ccgaggggat
    10081 cggcgacacg atccgcgtct ccctcacccc tcgccccggt ggcgaccggc gcgaggaggt
    10141 ctacgccgcg tgcgagctcc tccagtcgct cggcctccgc gcgttcgcgc cgagcgtcac
    10201 ggcctgcccc ggctgcggcc ggacgacctc gacgacgttc caggagctgg ccgagaggat
    10261 cgaagggtac gtgcgcgaga agatgccgct ctggaaggcc gagcgggagg gggtcgagac
    10321 gatgacgctc gccgtcatgg ggtgcatcgt gaacggcccc ggcgagtcga aggcggcgag
    10381 catcgggatc agcctccccg ggacgggcga ggcgccgagc tgccccgtct acgtcgacgg
    10441 ccagaagttc acgacgctga aggggtcgta cgaggagctc gcggccgcct tccaggcgct
    10501 cgtcgacgac tacgtcgcga cgaagtaccc cctccgctcc ggctccgccc gcgaggcatg
    10561 acgtgggccg tccgggcccg gcccgaacgc gataacctcc gcacccgatg aacgccgaaa
    10621 cgcccgaccg gctcgacccc cgcgccatgg tgaaggggac gatgctgagg gcgcacctga
    10681 gctgggccac gaaccgcttc ggggcgggct acgagcccgt ccggcagcgc ctctccgagc
    10741 cggtcgtcgc cctcctctcg cgccccgtcc tcccgaccga ctggatcccg gtctcgtcgc
    10801 tcatcgagct cgaccgcgcc atcgcggacg ccgccggaga gcctcccgag acgacgtggc
    10861 tcgccctcgg gcggcactcg gccgcgctca acctgacggg cgtctacaag agcttcatca
    10921 gcagcgagcc gcatcgcttc ttcgagcgga tgacggtcct gcaccaccag ttccagtcgt
    10981 tcgggaagtc ggtctacgag aggaccgggg agcgctcggg ccggatccgg atcgagagcc
    11041 cttcgacgta ctcgcgcgtc tactgcgtct ccggaaaggg ctactacgag gaggcgctcc
    11101 gtctcctcca ggcgccgggg ccgatcgtcg tccgcgagac cgagtgcgcc acggcaggcg
    11161 ccgccgcctg cgtgttcgag ctcgcctggt gagcccggag gtccggggag aggattctcc
    11221 cgccgccgac ctcacgctct tcgagcaggt cgcgcgggcg atcgacgact tcgtcctcgt
    11281 cgtcgacctc cagggacgcc tgacccacgt caacgacgcg gccgtcgagc gctcgggctg
    11341 gctccgggaa gagctcctcg ggaagccgtg gaccgtcttc ctgccgcccg ccgagcggga
    11401 cgagctggcc gcccggatcg ccgacgagac ccgcgcgggg ggcttccggg gcgacgtgaa
    11461 gaacgtcacg aagtcgggca aggcgctctg ggtcgcgctt cggacctcgg tcctgcgccg
    11521 ggacgggaag gcggtcgggt tcgtctccat ctcccgggac atcggggagc agaagcgggt
    11581 cgaggccgag ctgactgccg cgcgcgacgc cgccgaagcg gcgagccgcg ccaagagcga
    11641 gttcctcgcg aacatcagcc acgagatccg gacgccgatg aacgccgtca tcgggatggc
    11701 gacgctcctc ctcgacaccg cgctgacgcc cgagcagcgc gactacgtcg agacgatccg
    11761 cacgagcggc gacacgctcc tcgccctcct gaacgacgtc ctcgacttct ccaagatgga
    11821 gtccgaccgg ctcgagctgg aagcgcggcc gttctcgttc cgggaggccg tggaggactc
    11881 cttcgacctc gtcgcccctt ccgccgcgtc gaagggcctc gacctcgcgt accgggtcga
    11941 cccgtccgtc cccgcgagtc tcctcggcga cgtgacacgt gtccgtcagg tcctcctgaa
    12001 cctcctgacg aacgcggtca agttcacctc ctcgggcgag gtcctgctcc gcgcctcggc
    12061 tcgtccggcg gccgaggggt cccatcgcgt ggagatcgtc gtccgcgaca cggggatcgg
    12121 aatcccgaag gagcggctcg accggctctt ccggagcttc agccaggtcg acccctcgac
    12181 gacgcgccag tacggaggga cgggcctcgg cctggcgatc tgcaagcggc tcgccgagaa
    12241 gatgggagga ggcatcgagg cggagagctc gggtcggggc tcgacgttcc gcgtcgcgct
    12301 cgtcgtcccg gacggccctc cccgtcgcga cgcccccgtt cccggcatcg accgactggc
    12361 cgggcagaga gtcctcatcg tcgacgacgg accggcctcg agggacacgc tcttcgaaac
    12421 cctctcgggc tgggggatgc agccgcgggc cacgtcctcg ccacgcgagg ctctcgcgtg
    12481 ggtcggcgcc ggggagccgt tcgacctcgg gctcatcgac gaggagatgg ccgaggtgga
    12541 cggtcggagg ttcgccgagt cgctccggcg cttccgcgac gcggacgccc tgcccctcgt
    12601 cctcgtcacc tctcgcggcc ggcgggccgg cggggcacgg gcgccgttcg cggccgtggt
    12661 ggcgcggccc gtccggcccc gggccctcgc gtcggtcctc gcgggcctcc gcgacgacag
    12721 cgtctcgcgg cgtcggcgcc ggtcctcgcc cgcgctgccg aaggagctcg gggtctccca
    12781 ccccctccgc gttctcctcg ccgaggacaa cgccgtcaac cagaaggtgg ccctctccta
    12841 cctcgcgcgg atgggctacc gcgccgacct cgcccgcacg ggcatcgagg cgctggaggc
    12901 agtccggcgg aacgtctacg acgtcgtcct gatggacgtc cagatgccgg agatggacgg
    12961 cctcgaggcc gcacgccgca tccgaagctt cgccccggcc gagaatggcc cctggatcat
    13021 tgccctgacg gcgagcgcga tgacgggcga cgcggagcgg tgcctggcgg ccgggatgaa
    13081 cgactacctc gccaagccgg tccggggcga ggacctcgcg gcggccctcc gccgcgcccc
    13141 gcgctccgcc tcggcgaccg ggccggcctc cgccctcacc gccggccggc tggtcctcct
    13201 cccgggcccc ctcgcgaagc tgcgggagct ggagcggccg ggggagacgg gcttcctcgc
    13261 ctccctcctc gagatcttcc gccgcgacac gccgatccgg ctcgaggaga tggagcgggc
    13321 cgccgctacg gcggatgccg aagcgctgcg cagggcggct cacacgctca agtcgagcgc
    13381 cgcgactctc gggggcgccc gcgtcgagac gctcgcggcc cgaacggagg tgctcgcaat
    13441 gaacccgacg acgctcgccg aggcagcggc cctcattccg gccctccgga gcgagtccgc
    13501 ggccctgctg gccgccctcg ggctggccga gggcgaggag ggcgcccggc tctccctcga
    13561 tccgggcgac gacaggcgtt cctgagccgc cctcggggag cccggaagca ccttccgcgg
    13621 cgatagactc ttcgatgcgc gagcacccct gcgagggcgc gcgcgcggga gcgtaatccg
    13681 atgagcgcgt acaagatcgc atggcttccc ggcgacggtg tcggcaacga ggtcatggag
    13741 gccgcccgca tcaccctcga cgccctgaag ctggacgcct cctacacgca cggcgacatc
    13801 ggctgggagt tctggcgcac ggagggagag tcgttcccgc agcggacgat cgacctcctc
    13861 tcctcctccg acgccgcgat gttcggggcc atcacgagca agcccgtgaa ggcggccgag
    13921 caggagctcg ctcccgagct gcagggcaag ggcctcacct accgctcccc gatcgtgagg
    13981 atgcggcaga tgttcgacct ctacgtctgc ctccgcccgt gcaaggccta cccgggcaac
    14041 ccgctcaact tcaaggaggc gatcgacctg acgatcttcc gcgagaacac cgaggacctc
    14101 tacgccgggg tcgagttctc gcccgtcccc gacgcccttg ccacgacgct cgcggcgctc
    14161 agcaagccgt tcggccactt cgccgacctg ccgagcgacc agtacgcggt ctcctgcaag
    14221 gtgaacacgc ggaagggctc cgagcggatc gcccgcgccg ccttcgagta cgcgcggaag
    14281 ttcggccgga agaaggtgac gatcgtccac aaggcgaacg tcgtgagggc cacggacggc
    14341 ctcttcttcg aggaagcgaa gaaggtggcc gccgacttcc cggagatcgc cgtcgacgac
    14401 gcgaacgtcg acgcgatctg catgtggctc ctgaagaacc cgatgagcta cgacgtcctc
    14461 gtcgcgccga acctctacgg cgacatcatc tcggacctcg cggcccagat ggtcggcggc
    14521 ctcggcttcg gctgctcggg gaacatcggg aagaagctcg ccgtcttcga gccgagccac
    14581 ggctcggcgc cgaagtacgc cgggcagtac aaggtgaacc cgctcgcgac gatcctcgcc
    14641 gccaagatga tgctcgactt cctcggcgag cccgagaagg gggcgaagct cgaggcggcc
    14701 gtcgcgaagg tcatcgccga ggggaaggtc cggacgtacg acatgggcgg ctcggcgacg
    14761 accctcgaga tgggcgaggc cgttgcccgg gcgctctgac gtggcggcgg accgcatcgt
    14821 cctccacgag gtcggcccca gggacggcct ccaggccgag aagacggtcg tcccgacgga
    14881 gacgaagctc cgctggatcg gcgcgctctg cgaggccggc ctcgacgtcg tccaggtcgg
    14941 ctccttcgtc cacccggaga aggtgccgca gatggccgac accgacgctc tcttccgggc
    15001 gcttacggcg ccgggggcgg agcggaaggg gcacgcgatc tactccggcc tcgtcctgaa
    15061 cgagaagggg ctcgaccgcg cgctcgcctg cggcgtcgag atggtctgcc tcggcgtctc
    15121 ggcctccgag acccacagcc gcaagaacac cgggatgggg acggccgaag cgacggagcg
    15181 gatcgtcgcg acggcgagga aggccgtcga agcgggccgg aaggtccagg tctcggtcca
    15241 gtcggccttc ggctgcggct tcgaggggca cgtcccggag gagagggtga tcgacatcgc
    15301 ccgcgcctac gtcgacgccg gtctcgtgac gatcgccctc gccgacacgg cgggccatgc
    15361 ctaccccgag caggtcgagc ggctctacgg aaagctcctc gcgctcgccc cggggatcga
    15421 ggccgcctgc cacctccacg acacctacgg cgccggcatg gcgaacgtct acgccgcgct
    15481 ccgcgccggc gcggtctccc tcgagtcctc cttcgccggc ctcggcggct gccccttcac
    15541 gaaggtcgcc gccgggaacg tcgcgaccga ggacctcgtc cacaccctcc agcgcgacgg
    15601 gaagcggacc gacgtcgacc tcgaaggcct gatcgccgtc gcgaaggacg tcgaggcgtt
    15661 cctcggacgc gagatgcccg gccgcgtcca ccgcaccggc ccggtcccgc agcttcggaa
    15721 acaggaggcc tccgtatgac gaggaaggcc ctcgagggcg tcgtcgtcct cgacctgacg
    15781 aacgtcctct ccggcccgtt cgcgacgctc cacctcgccc tcctcggggc cgaggtgatc
    15841 aaggtcgaga acccgacgga cggcgacctg gcgcgcaagc tcggcaacgt cccgaagctc
    15901 aacaaggagc tgatggggac gagcttcctc gcgcagaacg cgaacaagaa gtcgctcacc
    15961 ctgaacctca agaagcccga ggcgaaggag atcttcaaga agctcgcggc gacggctgac
    16021 gtcgtcgtcg agaacttccg ccccggcgtc atgaggcggc tcggcgtcgg ctgggagact
    16081 ctccgcgtga tcaacccgcg cctcgtctac tgcgccatct ccggcttcgg ccaggacggc
    16141 cccgacgccg acaagcccgc ctacgaccag atcatccagg ggctctcggg cgagatggac
    16201 gtgaacggcg acgagcggct ccacccgctc cgggccggct tcccggtctg cgacaccgtc
    16261 ggcgggatga acgccgcgtt cgccatcctg gcggcgctct accaccgtga gcggacgggc
    16321 gaggggcagg cgatcgacgt cgccctcctc gactcgatca tgccgctcat ggggtgggtc
    16381 gccgcgaacc tcctcatcgg gggcgccccg cccgtcgcga tcgggaacga caacttcacg
    16441 gccgccccgt cggggatgtt ccggacgaag gacgggcaca tcaacatcgc ggcgaacaag
    16501 caggagcagt gggagtcggt ctgcgacgtc ctcggcgtcc cggagctgaa ggccgatccg
    16561 cgcttcgccg agcgcgacac gcggaagaag aaccgccacg cgctgacgcc gctcctcgag
    16621 gtgaagctcg ccgagcgccc cacgtccgag tgggtcgacc tcctgaacgc gaaggacgtc
    16681 ccctcgggcg acatcctgtc actcgaagcc gcgctgaagc agccgcaggt cgtccaccgg
    16741 aagaccctgg cgagcgtcga gaccccgggc gtcgggacgc ttcccctctt caacctgacg
    16801 gcgaaactcg agaagacccc cggggtgatc gacgccccgc cgccgcgcct cggcgcgcac
    16861 accgcagaga tcctcggtcg gatcggctat tccacggacg acgtggcgcg gctttcggcc
    16921 gagggcgtcg tctaaggaga agcccatggg caagaccgtc gtcgagaaga tcctggcacg
    16981 cgccgcgggc ctccccgagg tgaccgccgg ggacgtcgtc gagccgcgcg tcgacgtggc
    17041 gatgagccac gagaacgccg cgctcgtcat caaccagttc ctcgagatct acgaggggac
    17101 gggcgtgacg ccgcacccct gggacgtctc gaagatcgcg atcatcttcg accaccgcgt
    17161 cccggccgag tcgccgaaga ccgcgtcgaa ccagaagaag atccgcgagt tcgtcgac
//
LOCUS       DAOONL010000073        16996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3523_length_16996_cov_17.993743, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000073 DAOONL010000000
VERSION     DAOONL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16996
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_16996_cov_17.993743"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3077)
                     /locus_tag="PLP50_11340"
     CDS             complement(<1..3077)
                     /locus_tag="PLP50_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPA52185.1"
                     /translation="MTTEPRVRSVFVLSAVVVLLAGGALALGVKPGAAKGVKPVPMAT
                     STWNEVDRLVNEQKLEEAAKSAEAILAAAMAAKDEPEWTKALVKVTQLRIGLHGYETA
                     VRRLREEPWPAGPLHRAQLSLLYGHSLVTYQRAYSWEIGQREKVDTKGEVDLKAWTRE
                     QILAEALKAALDVWAIRSDLGKEPASAAALVLVPNDYPKEIRGTLRDAVSYLLVDLLA
                     DTAGWTPEQSNEVWRLDLGALLAGDAGREAKVDLASAEVHPLVRIGAVLTDLEAWHTK
                     EGRTGARLEARLERVRRLAASFTEERERERIRKDLEGRLVAFRSDPWWAMGKAEVAEL
                     HRSGARPNRLVEARREAEEGRAAYPTSPGGARCLAIGKAIEAPEYALQAMAVDGAKKR
                     SVEVSHRNLEALHFRAYRVDAEKWIAASKDWSALPTSEDMRKLVGGTPAVEWTSALPR
                     TPDFETHRTFVTPPLPEAGFYVVLASARKDFRESDNIVVGTNLFVSDLVLAVRPEPDT
                     GSVMARVLSGATGHAVAGAEVTLWRFDWREGHRRDETKVSGADGRVSFALREKGGAFH
                     LFAKKGRDVTFDGQQVGFHRESKPGLRSDSLLYTDRSIYRPGQTLHWKVLAYSGSTED
                     ARFRAAARTPVTVWLVDPNGERVETRTVSTNDFGTAAGEMVVPTGRLLGAWRLQSSLS
                     GSTGIRVEEYKRPTFEATFLEPKEPMRLNRKATLVGEARYYFGLPVTNGAVKWRVTRE
                     TLRPWWWAFWGFGSAAKPQVVAVGETTLDADGTFRVAFTPEADEAKADSKETTWRYVV
                     QADVLDEGGETRSATRAVRLGFVSVEADVAFPAGFGRAGEKLSARITRTNLDGVPRSG
                     NGTWRLIALASPPETPLPADLPRRTEESGEEGSRFRTPGDALRPRWDTAFRVEAVLRD
                     LPDGAEKAHGEAKHGADGVAALDLGTLPAGAWRIRYETVDDFGAKYETSRDVIVAGGK
                     TTPPVPLVLAAESGSVAVGGTARLLAASGLPGQEMLLERFRGGKLLSRTLLTAG"
     gene            complement(3156..3905)
                     /locus_tag="PLP50_11345"
     CDS             complement(3156..3905)
                     /locus_tag="PLP50_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPA52186.1"
                     /translation="MKPARRLSGLLSILGASLAASCGTLEPTSAAGLSARRFEGTVSY
                     RVAYRYLLYLPEGYVRDPASRWPLLLFLHGSGERGDDLALVKRHGPPKLLASGGALPF
                     VVVVPQCPEGERWRADALAALLDDVTRRLRIDAERVYVTGLSMGGRGTWDLAMSYPGR
                     FAAIAPVCGGALPDRACNLKGVPIRAFHGAKDDVVPLSESQTVVEAVRKCGGSAELVV
                     FPEVGHDSWTAAYSDPSLWDWLLSRRRRSAN"
     gene            complement(3949..6366)
                     /locus_tag="PLP50_11350"
     CDS             complement(3949..6366)
                     /locus_tag="PLP50_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPA52187.1"
                     /translation="MARDAAPSGAPRIGTKVLHKFNRDLGPGRVEEVAARRVVVFFPR
                     SGERLTFGIADMGLRPLELSAGRDARIEETGEVVTLASRERGGTAWTTTDGRVIEEEH
                     LWPAEIPQDPVERLAGLDVDRAAAFRNRVDGLFLARMRQARGLGSFLGGRIAIHPHQL
                     HVAERATAYEPVRWLLADEVGLGKTIEACLILSRLVRTDRADRVLVVAPSTLTVQWLG
                     ELWRKFHQVFVLLDAKRRADVAKDHGPEFNPFDAHRRSVVALEDLASEPGLAQKAVEA
                     GLDLLVVDEAHRLVLEAEAERAVAPLAKAARHLLLLSATPLEADTSGFFRLLQMVRPD
                     AYRSEKEFVAALSENQPLPPCTSATRRVDIGGLPPRVPLPVELDRVPSSQALSGDEKA
                     DAADPRVEWLVGKAKGFAPGGVEEGKTLVFCHDLPTLQSLKTLLERETRKRVAVFHEE
                     LSPDRRDLEVAEFRRPKGPTFLISTECGGEGRNFEFCRRLVLFDLPLDPAQVEQRIGR
                     LDRISRKERVEIVYFRPPTGFWRDLVELYERIGLFREPLGGLERSLSSVEAAIRKAEK
                     ASALGRAELPVEELVAQVRGAAQARQRAAYHHLHAEGFRPEHASGILSRVPAGLDAAM
                     ERFVIAACELYGFDVAEKKGSRTWYLEFGGDALLEHLPGVPGGSRFLGTFDREEAVRH
                     EEHDFFASGHPLVEGIFQELEDGPRGRAALVKLEKSGVAGEGVLFLHRREGGFAASAV
                     DLSGTSRPEWAELLLRGRASLRGISVADWSPTIPRGRGWAATVRTLAARAEKNGPVLA
                     AAGFRLS"
     gene            complement(6437..8773)
                     /locus_tag="PLP50_11355"
     CDS             complement(6437..8773)
                     /locus_tag="PLP50_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPA52188.1"
                     /translation="MTEPTAPSPAPVPEPEPTPPADVPPAEGDRAEIEEIPGYDGHAK
                     VDRPSQDPIAPPPEDPDVPETPFEKVLRELPYLPATYDETYLYLVPCDPKSLYVIWEI
                     SAEAREALKARFGADFFEKNALLLRVYDVTGVSFDGFNANSLFEVDDYLADKSEYWVN
                     VAGGRDYVAELGYRALGTTYFEKVARSNAVFVPRGVDEPAEMHVSWASIDVPHHDVEV
                     PVGPDQWRFNQYLYWKRRSHAAPEEKGYWSLVLHQHLPFVRHPEYEVGLEEQWFFEAV
                     VSVYTQLLHVMWNLERDKVDFRLTVSLTPPLLSMMQDPHLKLRAARHIDECIKLATRE
                     RDNARGKPWLAAAEQILSRFWTAKHVWDAYEGDLTRGYRDFQNAGKLEVITCPATHWI
                     LPLYKDFPEAIRGQVRTAKRQYERVFGRSPRGIWLAENAWTPGLDSFLAEEGIRWFLV
                     NPKGLREGDTRPFFDSANPVITPAGTAAFPIDPETRAKIWSRENGFPGHPNYKEWYRD
                     LGYDADWDYLTDYWKTAGVRRNTGIKYYRITGRGADLGGKTYYVPEWADETREAQAAQ
                     FVFERGAQATWFQEKHGGRKPCVVSAYDAELFGHWWEEGPGFLESVFRKLLWDQSVVR
                     PVTPSEYLSENPHHQRMTPGASSWGKGDYFQTWVEGREYQPNTWVYRHLFRLVGKMAD
                     LATRHRDTADLLLKRALDQSARCLFLAGASDWGFLIETGQAVRYSEVQIVRHLDRARE
                     LMRQVDAGKIHEDYLTILEDADTIFPYGDMDFRVFCRS"
     gene            8772..9110
                     /locus_tag="PLP50_11360"
     CDS             8772..9110
                     /locus_tag="PLP50_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52189.1"
                     /translation="MAWVASFFLVGFRAGVLRAGFAAGAFRSVAAGFASWVLPACVFR
                     TTRPFGAASAGVFARRFGVAAGAASRGGAVSGEGFGVLGPAFGFRGPGPSFFVVRFGF
                     SSMVLVPFGS"
     gene            complement(9231..10352)
                     /locus_tag="PLP50_11365"
     CDS             complement(9231..10352)
                     /locus_tag="PLP50_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPA52190.1"
                     /translation="MRIETLAVHAAYSVDPATGAVAPPIHLSTTFGRDEAGVPLAGHT
                     YIRESSPTQDLLEEALARLEGGEAALVFGSGMAAGLAVVQALPPGSHVVLPDDAYYGY
                     RVAARDFFPKWGLEATFAPLDRPGALEAALRAETRLVWLESPSNPLLKVTDLARAASV
                     ARAAGALVLVDGTFATPILQRPIELGADVVLHATTKGMGGHSDVAGGALVFAARGELL
                     EKVRHVRTVLGAVASPFNSWLVLRGLRTLAVRARVQSETALAVAKALEAHPGVSKVNY
                     PGLGSHPGHDVARRQMSAFGSMISFHVAAGREAAIAVVGRARLFTRATSLGGVESLIE
                     HRATSEGEASTAPPDLVRLSIGLEHPDDLVADLDAALRT"
     gene            complement(10382..11599)
                     /locus_tag="PLP50_11370"
     CDS             complement(10382..11599)
                     /locus_tag="PLP50_11370"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA52191.1"
                     /translation="MPALVAAFTPLRHRDFRLLWSGSLVSNVGTWMQRVAQGWLVLTL
                     TGSPFWLGVDAFLGEAPFLAFSLFGGVLADRAERRRILLVSQVVQMASALVLMTLIAT
                     GRITLGAILATSVVVGLAQAFGGPAFQALVPMLVPPADLPRAILLNSIQFNFARVVGP
                     AIGGLAFARFGAAGCMGLNGLSFLAVVAALAAIPRRPGTGSGGVTVLDGLREGVATVW
                     RRPSLRGLVALAFLGTFCSNPLLTFLPVFARDVFHAGAERYSVLLAAFGCGAVVGGLA
                     VAGAARLPRRGLLSALGLVLYGLLMVAFALCREPTFSLVLLVAAGACQMVVFSGFMTL
                     VQTSVGDALRGRVVSIYSLAFRGGMPLGNLSAGTAAAAVGAPAVLVVLGGALAIAGGV
                     VARRRGRDGVASI"
     gene            11713..12735
                     /locus_tag="PLP50_11375"
     CDS             11713..12735
                     /locus_tag="PLP50_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPA52192.1"
                     /translation="MLRTPVVFLTGASGEIGHTLIERLSEDGSRPVITLDLKPLDPSI
                     APRVARHFTGSILEPQLLERILSEFEVDQVFHLAALLSTRSEFTPVTAHQVNVEGTMA
                     MLEFAQKQGESHGRPVVFLYPSSIAAYGMPGLDVKAKAGKVKEDDFTNPTTMYGCNKL
                     YCEQLGRYYARHYKQLAPETLSGKVDFRSIRFPGLISAFTVPSGGTSDFAPEMIHAAA
                     QGKPYACFVRPDARIPFMAMPDAVDALLQLAAAPREKLTRTTYNIGAFAPSAAEIQAI
                     VEKAFPGAEITTKVDAKRQGIVDSWPADVDDSAARKDWGLSPRYDLDRAFQEYLIPNI
                     RKAYQG"
     gene            complement(12755..13687)
                     /gene="hflK"
                     /locus_tag="PLP50_11380"
     CDS             complement(12755..13687)
                     /gene="hflK"
                     /locus_tag="PLP50_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPA52193.1"
                     /translation="MTRTVRRALFAASGLAFGAWLLAGVSALRPAQQGVVLRLGAVQP
                     LPLGPGIHWRPAGIDRVVKVEVTKTETVPIGFRTADGGRAVSLPADEGQWLTGDTNIL
                     AVQLAVQYRVARPVEWVVHGRDTADVVRRAAESVVTEAMGVLPVDDALTGGRHTLLEE
                     VRRGTQELLDRWQSGVAVVSVALRSVDPPPPVLAAFQDVQDAKSDRERYVNEAESYAN
                     DTLPRARGEAEALVSAASSQRQRRVEEAHGDAARFDALRREASLAPGPLRARLYLETL
                     EKVLPRMRLYVVDEATRLRLVKPEPAASAGEPGG"
     gene            complement(13684..14619)
                     /gene="hflC"
                     /locus_tag="PLP50_11385"
     CDS             complement(13684..14619)
                     /gene="hflC"
                     /locus_tag="PLP50_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPA52194.1"
                     /translation="MEETLEPPARSRRRRVAAVAAGAILLLLIARVPYVVEATEFAVR
                     TRFGRPVAVVTEPGLHARVPLVDDVARLDARLLYFDPPAGEFLTNDKKNIVVAPVVLW
                     RIDEPLKFLQTLVTRESAEARLADLVASETGAALGAVPFTRLVSATEGEADLAGVVAG
                     IGERVRARAAADYGVKVVDVRLKRIAFPEQNLLSVFGRMRSERERIARRFRSEGEEAA
                     LKIRAEADREKTRLLSEAYRRAEEEKGAGEAEAARIYARATNQDPELYKFLRTLRAYE
                     SILDEKTTLVLPGDSDVLRLLTDGAAPPPPRKGPN"
     gene            complement(14630..16822)
                     /locus_tag="PLP50_11390"
     CDS             complement(14630..16822)
                     /locus_tag="PLP50_11390"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HPA52195.1"
                     /translation="MSLLVRIFEETYRIVLDSGFFILLGFLLAGLLHELVDTARIGRA
                     LGGRSLGAILKAAVIGGPLPLCSCGVLPMAVALRKKGASREATVSFLISTPETSEEAI
                     LLTWGLLGPVMALVRPVVGVVTAIVAGLVSLVAGGEDDRRSTEAGEAADGPAVPCSTE
                     DGCGATETPAARSGTLRERAARVFRYAFVTMVDELAFWMLLAFLATGVLAGVLPEDFF
                     LRFLPWPLLTMVLMALVGLPMYVCASASTPIAAAMIAKGLDPGSALVFMLAGPATNAS
                     TIAVVARMFGRRFVGIYVGSIVGVAIASGLLLNAVVGAGLVPAPGSPEAESGGAWAVV
                     KFLSAFAFLALMAFSFQRQGFRPGWNELKGHARAFRDLVREQAPRRLPRRALAGLAAT
                     LLLLSWGRTALLVVRPDETGLVRTLGKVTASDLAPGLHLVPPFPLGKAEAILTARLRS
                     VEIGFRYRATGSAAAPVTSRVFVPTQSTRVPEEAQFLTGDENVIEVTAVVHYRVADPA
                     AFRFGIDRAEPAFRDLARALLVEEVGRTPIDAIYTAERGAVERSVLEALRKAPVLRET
                     GLAPVDLRLLFVHAPDDVHGAFRDIASAAEDRTTARNKALVEAEGALGQARGEVARTL
                     AEAGSERTRLVEHAKGDAAAFVPLAREVAAAPRTSRLRLKLEALERVLSGVPKLIRPD
                     PRRAPGFELWVTPALPGEAASVPAPARGLAGFVNPIPAPPPGVPAAER"
BASE COUNT         2439 a         6152 c         6126 g         2279 t
ORIGIN      
        1 cccgccgtca ggagcgtgcg cgagaggagc ttcccgccgc ggaaacgctc gaggagcatc
       61 tcctgccccg ggagccccga ggccgcgagg aggcgcgccg tcccgccgac cgcgacggag
      121 cccgactcgg ctgcgaggac gagagggacc ggcggagtcg tcttcccgcc ggcgacgatc
      181 acgtcgcggg aggtctcgta cttcgctccg aaatcgtcga cggtctcgta gcggatccgc
      241 cacgcccccg ccgggagggt gccgaggtcg agagcggcca cgccgtcggc accgtgcttt
      301 gcctctccgt gcgccttctc ggcgccgtcg ggcaggtcgc gaaggaccgc ctccacccgg
      361 aacgccgtgt cccagcgtgg gcgaagcgcg tcccccggcg tccggaagcg acttccctcc
      421 tccccggact cttcggtccg ccgcgggagg tcggccggga gcggcgtctc gggcggcgag
      481 gcgagggcga tcagccgcca cgtgccgttc ccagatcgcg gcacgccgtc gaggttcgtt
      541 cgggtgatcc ttgccgacag cttctccccg gcgcggccga aacccgccgg gaaagcgacg
      601 tcggcctcga cggagacgaa gccgagccgg acggcgcgcg tcgcgctgcg ggtctcgccc
      661 ccctcatcca ggacgtcggc ctgaacgacg tagcgccagg tcgtctcctt cgagtcggcc
      721 ttcgcctcgt ctgcctccgg cgtgaacgcc actcggaacg tcccgtcggc gtcgagggtc
      781 gtctcgccga cggcgacgac ctgcggcttc gccgcggagc cgaagcccca gaacgcccac
      841 caccagggcc ggagcgtctc gcgcgtgacg cgccacttca cggcgccgtt cgtcaccggg
      901 aggccgaagt agtaccgcgc ctcgccgacg agcgtcgcct tccggttcag gcgcatcggc
      961 tccttcggct cgaggaacgt cgcctcgaac gtcggccgct tgtactcctc gacccgtatc
     1021 cccgtgcttc cggacaggga gctctggagc cgccaggcgc cgaggaggcg tcccgtcggg
     1081 acgaccatct cacccgccgc cgtgccgaag tcgttcgtcg agacggtccg cgtctcgacc
     1141 cgctccccgt tcgggtcgac gagccagacc gtgaccgggg tccgcgccgc cgcgcggaag
     1201 cgggcgtcct ccgtcgagcc gctgtaggcg aggaccttcc agtggagcgt ctggcccggc
     1261 cggtagatgc tccggtcggt gtagaggagc gagtcgctcc gaaggcccgg tttcgactcg
     1321 cgatggaagc cgacctgctg gccgtcgaac gtgacgtcgc gtcccttctt cgcgaagagg
     1381 tggaacgcgc cgcccttctc ccgcagcgcg aacgagaccc gcccgtcggc gcccgagacc
     1441 ttcgtctcgt cgcgccggtg cccctctcgc cagtcgaacc tccagagcgt cacctcggct
     1501 ccggcgaccg cgtgccccgt cgcacccgag aggacacgcg ccatcacgga gcccgtatcc
     1561 ggctcggggc gcacggcgag gacgaggtcg gagacgaaga ggttcgtccc gacgacgatg
     1621 ttgtccgact cgcggaagtc cttccgggcc gaggcgagga cgacgtagaa ccccgcctcc
     1681 ggaagaggcg gcgtcacgaa cgtccggtgc gtctcgaagt cgggtgtcct cgggagggcg
     1741 gacgtccact cgaccgccgg cgtccctccc acgagcttcc tcatgtcttc cgaggtaggg
     1801 agcgccgacc agtccttcga cgcggcgatc cacttctcgg cgtcgacgcg gtaggcccgg
     1861 aagtggagcg cctcgaggtt ccggtgagag acctcgacgg agcgcttctt cgccccgtcg
     1921 acggccatcg cctgcagcgc gtactcgggc gcctcgatcg ccttcccgat cgcgaggcac
     1981 ctcgccccgc cgggggaggt cgggtaggct gcgcgcccct cttccgcctc ccgccgcgcc
     2041 tcgacgagcc ggttcggccg cgcgccgctc cgatggagct cggcgacctc cgccttcccc
     2101 atcgcccacc acggatcgga gcggaacgcg acgaggcgcc cctcgaggtc cttccggatc
     2161 cgctccctct cgcgctcttc ggtgaacgat gccgccagcc tccgcaccct ctcgagacgc
     2221 gcctcgagcc gcgctccggt ccgcccctcc ttcgtgtgcc acgcctccag gtcggtgagg
     2281 acggccccga tccgcacgag cgggtggact tcggcggagg cgaggtccac cttcgcctcg
     2341 cgcccggcat cccccgcgag gagcgccccg aggtcgagcc gccagacctc gttcgactgc
     2401 tccggcgtcc agccggcggt gtcggcgagg aggtcgacga ggaggtagct caccgcgtcg
     2461 cggagcgtcc cgcggatctc cttcgggtag tcgttcggca cgaggacgag cgcggccgcc
     2521 gaggccggct ccttcccgag gtcggagcgg atcgcccaga cgtccagcgc cgccttcagc
     2581 gcctcggcca ggatctgctc ccgcgtccag gccttcaggt cgacctctcc cttcgtgtcg
     2641 accttctcgc gctggccgat ctcccacgag taggcccgct ggtaggtgac gagcgagtgg
     2701 ccgtagagga gcgagagctg cgcccggtgg agcggcccgg ccggccacgg ctcctcgcgg
     2761 aggcgccgga cggccgtctc gtagccgtgg aggccgatcc ggagctgcgt gaccttcacg
     2821 agcgccttcg tccactccgg ctcgtctttc gccgccatcg cggccgccag gatcgcctcg
     2881 gcggacttcg ccgcttcctc gagcttctgc tcattcacga gacgatcgac ctcattccag
     2941 gtggaggtgg ccatcggcac gggcttcact cccttcgcag ctcccggctt gacgccgagc
     3001 gcgagcgcgc ctcccgcgag gagaacgacg acggcggata ggacgaatac ggaccggacg
     3061 cgcggctctg tcgtcataac gggagcttag accgctccgc cccccggaag ttcccccgat
     3121 gcgctgcgac ttggtgccag gcgtgaaatc gtgtattagt ttgcggatcg acgacggcgg
     3181 gagaggagcc agtcccagag ggacgggtcg gagtaggccg ccgtccacga gtcgtgtccc
     3241 acttccggga agacgacgag ctccgccgag ccgccgcact tccggactgc ctcgacgacg
     3301 gtctgcgact cggagagcgg gacgacgtcg tccttggcgc cgtggaaggc ccggatcggc
     3361 acacccttca ggttgcaggc gcggtcggga agcgccccgc cgcagacggg tgcgatcgcg
     3421 gcgaagcggc ccggatagga catcgcgagg tcccacgtcc cgcgccctcc catcgagagt
     3481 cccgtgacgt agacgcgctc cgcgtcgatg cgcagccgcc gggtgacgtc gtcgaggaga
     3541 gcggcgaggg cgtcggcccg ccagcgctcc ccctccgggc actgcggcac gacgacgacg
     3601 aacggcaacg ctccgccgga ggcgaggagc ttcggcgggc cgtggcgctt cacgagagcg
     3661 aggtcgtctc cccgctcgcc cgacccgtgc aggaagagga gcagcggcca ccggctcgcc
     3721 gggtcgcgca cgtatccctc cgggaggtag aggagatacc ggtacgcgac ccggtaggag
     3781 accgtcccct cgaagcgccg ggcgctcagc cccgccgcgg acgtcggctc gagcgtcccg
     3841 cacgaggcgg cgagcgaggc gccgaggatc gagaggaggc cggagaggcg tcgcgccggt
     3901 ttcacgcgcc cattttgccg cgcaacgcgg ccggacgtgc gcggccgctc aggagaggcg
     3961 gaagcccgcc gcggctagga ccgggccgtt cttctcggcc cgcgccgcga gcgtccggac
     4021 ggtcgcggcc cagcctcgtc cgcgcgggat cgtcgggctc cagtcggcga cggaaatccc
     4081 gcgcaggctg gcccgaccgc ggaggaggag ctcggcccac tcgggccgtg acgtgcccga
     4141 caggtcgacg gcggaggcgg cgaagccccc ctccctccgg tggaggaaga ggactccttc
     4201 tcccgcgacc ccgctcttct cgagtttcac gagggccgcc cgcccgcgcg ggccgtcctc
     4261 gagctcctgg aagatcccct cgacgagcgg gtggccggaa gcgaagaagt cgtgctcctc
     4321 gtgccggacc gcctcctcgc gatcgaacgt gccgaggaag cggctccctc ccgggacgcc
     4381 cggcaggtgc tcgaggagcg cgtcaccacc gaactccagg taccaggtcc gggacccctt
     4441 cttctccgcg acgtcgaagc cgtagagctc gcaggccgcg atgacgaagc gctccatcgc
     4501 cgcgtcgagg ccggccggga cgcgcgatag gatcccgctc gcgtgctcgg gccggaaccc
     4561 ttctgcgtgg aggtggtgat aggccgcgcg ctgacgggcc tgcgccgctc cccgcacctg
     4621 ggcgacgagc tcctcgacag gcagctcggc gcggccgagc gcagaggcct tctccgcctt
     4681 ccggatcgcc gcctcgacgc tcgagagtga gcgctcgagc ccgccgagcg gctcgcggaa
     4741 gagcccgatc cgctcgtaga gctcgacgag gtccctccag aagcccgtgg gcggccggaa
     4801 gtagacgatc tcgacgcgct ccttccggct gatccggtcg aggcgcccga ttcgctgctc
     4861 cacctgggcc gggtcgaggg gaaggtcgaa gaggacgaga cgccggcaga actcgaagtt
     4921 ccgtccctct ccgccgcact ccgtggagat gaggaacgtc ggccccttgg gccgccggaa
     4981 ctccgccacc tcgaggtctc gccggtcggg cgacagctcc tcgtggaaga cggcgacgcg
     5041 cttgcgggtc tcgcgctcga ggagcgtctt cagcgactgg agcgtcggga ggtcgtgaca
     5101 gaagacgagc gtcttcccct cctcgacgcc tcccggcgcg aagcccttcg ccttcccgac
     5161 gagccactcc acgcgcgggt cggcggcgtc agccttctcg tctcccgaca aggcctggga
     5221 ggacgggacc cggtccagct ccaccgggag cgggacccgg ggcggcagcc ctccgatgtc
     5281 gacgcgccgc gtcgcgctcg tgcacggagg gagcggctgg ttctccgaga gggccgcgac
     5341 gaactccttc tcggaccggt aggcgtcggg ccggaccatc tggaggaggc ggaagaagcc
     5401 cgaggtgtcc gcctcgagag gcgtcgcgga gaggagcagg aggtgacgcg cggccttcgc
     5461 gagcggcgcg acggcccgct cggcctcggc ctcgaggacg aggcggtgcg cctcgtcgac
     5521 gacgaggagg tccagcccgg cctcgaccgc cttctgcgcg aggcccggct cggaagcgag
     5581 gtcctcgagg gcgaccacgg aacgccggtg ggcgtcgaag gggttgaact ccggcccgtg
     5641 gtccttcgcc acgtcggcgc ggcgtttcgc gtcgaggagg acgaagacct ggtggaactt
     5701 ccgccagagc tcgccgagcc actggacggt gagcgtcgac ggagcgacga cgaggacccg
     5761 gtccgcccgg tcggtccgga cgagacgcga gaggatgagg caggcctcga tcgtcttgcc
     5821 gaggccgacc tcgtcggcaa ggagccagcg gaccggctcg taggccgtcg cccgctcggc
     5881 gacgtggagc tggtgcgggt ggatcgcgat ccggccgccg aggaacgagc cgagcccccg
     5941 ggcctgccgc atccgcgcga ggaacaggcc gtcgacgcgg ttgcggaacg cggctgcacg
     6001 gtcgacgtcg aggccggcga gccgctcgac cgggtcctgc ggaatctccg ccggccagag
     6061 gtgctcctcc tcgatgacgc gtccgtcggt cgtcgtccag gccgtcccgc cgcgctcgcg
     6121 ggaggcgagg gtcacgacct ctccagtttc ctcgatccgg gcatcgcggc ccgccgacag
     6181 ctcgagcggc cggagcccca tgtcggcgat cccgaaggtc agccgctcgc cgctccgcgg
     6241 aaagaagacg acgaccctgc gggccgcgac ctcttcgacc cgtcccgggc cgaggtcgcg
     6301 gttgaacttg tgcaggacct ttgtcccgat gcggggcgcc ccggacgggg cggcgtccct
     6361 cgccatgaga ccgggaagga tgcaccggcc ggtcccccgg ggcaaggccc cggggaagcc
     6421 cggccggttc aaggcgttag gaacggcaga agacccggaa gtccatgtct ccgtaaggga
     6481 agatggtgtc ggcgtcttcc aggatcgtca gatagtcctc gtggatcttc ccggcgtcga
     6541 cctgccgcat cagctcgcgg gcgcggtcga ggtgacggac gatctggacc tcggaatagc
     6601 ggacggcctg accggtctcg atgaggaagc cccagtcgga ggcgccggcc aggaagaggc
     6661 agcgtgccga ctggtcgagc gcgcgcttca ggaggaggtc ggccgtgtcg cggtgccggg
     6721 tcgcgaggtc ggccatcttc ccgacgagcc ggaagaggtg ccggtagacc cacgtgttgg
     6781 gctggtactc gcgaccctcg acccaggtct ggaagtagtc ccccttgccc cagctcgagg
     6841 cgcccggcgt catccgctgg tggtgcgggt tctccgagag gtactcggac ggcgtgacgg
     6901 gacggacgac cgactggtcc cagaggagct tgcggaagac cgactcgagg aagcccggcc
     6961 cctcctccca ccagtggccg aagagctcgg cgtcgtaggc cgagacgacg cacggcttgc
     7021 ggccgccgtg cttctcctgg aaccaggtcg cctgcgcgcc gcgctcgaag acgaactggg
     7081 ccgcctgggc ctcgcgcgtc tcgtcggccc actccgggac gtagtaggtc ttcccgccga
     7141 ggtcggcgcc ccggcccgtg atccggtaat acttgatccc ggtgttgcgg cggacgccgg
     7201 ccgtcttcca gtagtcggtg aggtagtccc agtcggcgtc gtacccgagg tcccggtacc
     7261 actccttgta gttcggatgg cccgggaagc cgttctcgcg cgaccagatc ttcgcgcgcg
     7321 tctcggggtc gatcgggaac gccgccgtcc cggccggcgt gatcaccggg ttcgccgagt
     7381 cgaagaacgg gcgggtgtct ccctcgcgaa ggcccttcgg gttgacgagg aaccagcgga
     7441 tcccttcctc cgcgaggaag gagtcgagcc ccggcgtcca ggcgttctcg gcgagccaga
     7501 tcccgcgcgg gctccggccg aagacccgct cgtactgccg cttcgcggtc cgcacctgcc
     7561 cccggatcgc ctccgggaag tccttgtaga gcggcaggat ccagtgcgtc gccgggcagg
     7621 tgatgacctc gagcttcccg gcgttctgga agtcgcggta cccgcgcgtc aggtccccct
     7681 cgtaggcgtc ccagacgtgc ttcgcggtcc agaagcgcga gaggatctgc tcggcggcgg
     7741 cgagccacgg cttgccccgc gcgttgtccc tctcacgggt cgcgagcttg atgcactcgt
     7801 cgatgtgccg ggccgcccgg agcttcaggt gcgggtcctg catcatcgac aggagcggcg
     7861 gcgtgaggct gaccgtgagg cggaagtcga ccttgtcgcg ctccaggttc cacatcacgt
     7921 ggaggagctg cgtgtagacc gagacgaccg cctcgaagaa ccactgctcc tcgagcccga
     7981 cctcgtactc cggatgcctc acgaacggga ggtgctggtg gaggacgagg gaccagtacc
     8041 ccttctcctc gggagcggcg tggctcctcc tcttccagta gaggtactgg ttgaagcgcc
     8101 actggtccgg cccgacgggg acctcgacgt cgtggtgggg gacgtcgatc gaggcccagg
     8161 agacgtgcat ctccgccggc tcgtcgacgc cgcgcgggac gaagacggcg ttcgagcggg
     8221 cgaccttctc gaagtaggtc gtcccgagcg cccggtagcc gagctcggcg acgtagtcgc
     8281 gcccccccgc cacgttcacc cagtactcgg acttgtcggc gagatagtcg tccacctcga
     8341 agagggagtt cgcgttgaac ccgtcgaagg agacgccggt cacgtcgtag acgcggagga
     8401 ggagggcgtt cttctcgaag aagtcggcgc cgaatcgcgc cttgagcgcc tcgcgggcct
     8461 ccgccgagat ctcccagatg acgtagaggc tcttcgggtc gcacggcacg aggtagaggt
     8521 aggtctcgtc gtacgtcgcc ggcaggtacg gcagctcgcg gagcaccttc tcgaacggcg
     8581 tctcggggac gtccgggtcc tcgggcggcg gcgcgatcgg gtcctgcgac gggcggtcga
     8641 ccttcgcgtg cccgtcgtag ccgggaatct cctcgatctc ggcgcggtct ccttccgcgg
     8701 gcggtacgtc ggcgggcggc gtcggctcgg gctccgggac gggagcaggg ctcggggcgg
     8761 tcggctcggt catggcctgg gtcgcctcct tcttcctcgt cggcttccgg gcgggcgtgc
     8821 tgcgcgccgg cttcgcggcc ggggccttcc ggtccgtcgc ggccggcttc gcgtcctggg
     8881 tcttgcccgc ctgcgtcttt cggacgaccc ggcccttcgg agccgcgtcg gccggggttt
     8941 tcgcgcggcg attcggagtc gctgcgggtg cggcctcccg ggggggggcg gtctcgggtg
     9001 agggcttcgg tgtcctcggc cccgctttcg gcttccgggg ccccgggccg tctttcttcg
     9061 tggtccgctt cggcttctcg tccatggtcc tcgttccgtt cggatcgtga tcccggcccc
     9121 gcgaggacac gacgtcccgc ctttgggcca gaggccggtc cctggttccc ggtctcgggt
     9181 ccttgtccgg cggcgtcccg atcatacggg atcgatcgtg aagaccgcgt tcaggtccgg
     9241 agcgccgcgt cgaggtcggc gacgagatcg tccgggtgct cgaggccgat cgacagccgg
     9301 acgaggtccg gaggcgcggt ggaggcctcc ccctcgctcg tcgcgcggtg ctcgatcagg
     9361 ctttcgaccc cgccgagcga cgtcgcccgc gtgaagagcc gcgcccgccc cacgacggcg
     9421 atcgccgcct cgcgcccggc ggcgacgtga aacgagatca tcgagccgaa ggcgctcatc
     9481 tgccggcggg cgacgtcgtg gccggggtgc gagcccagcc ccgggtagtt gaccttcgag
     9541 acgcccgggt gcgcctcgag cgccttcgcg acggccaggg ccgtttcaga ctggacgcgc
     9601 gcgcgcaccg ccagggtccg gagccctcgg aggacgagcc acgagttgaa cggcgaggcg
     9661 accgccccga ggacggtccg gacgtgccgc accttctcca ggagctcgcc ccgggccgcg
     9721 aagacgagcg cgcctccggc gacgtcgctg tgcccgccca tccccttcgt cgtcgcgtgg
     9781 aggacgacgt cggcgccgag ctcgatcggc cgctggagga tcggcgtcgc gaaggtgccg
     9841 tcgacgagga cgagagcccc cgccgccctc gcgaccgagg ccgcccgcgc gaggtccgtg
     9901 accttcagga gcgggttcga ggggctctcg agccagacga ggcgggtctc ggcgcggagc
     9961 gccgcctcga gggcgcccgg acggtcgagc ggcgcgaacg tcgcctcgag cccccacttc
    10021 gggaagaagt cgcgggccgc gacccggtag ccgtagtagg cgtcgtcggg gaggacgacg
    10081 tggctgccgg gcggaagcgc ctggacgacg gcgagcccgg ccgccatccc cgagccgaag
    10141 acgagcgccg cctccccccc ttccaggcgc gcgagcgcct cctcgagcag gtcctgcgtc
    10201 gggctcgact cccggatgta ggtgtgcccg gcgagcggga cgcccgcttc gtcccgcccg
    10261 aacgtcgtcg agaggtggat cggcggcgcc accgctccgg tggccgggtc gacggagtac
    10321 gcagcgtgga cggcgagcgt ctcgatcctc atgcggcgca gcgtacagga gccgccaccc
    10381 ctcagatcga ggcgacgccg tcccggcccc tccgacgcgc gacgaccccg cccgcgatcg
    10441 cgagcgcccc gccgaggacg acgaggacgg cgggcgcgcc gaccgccgcc gcggccgtcc
    10501 ccgccgagag gttcccgagc ggcatcccgc cccggaacgc gaggctgtag atactgacga
    10561 cccggccccg gagcgcgtcg ccgacgctcg tctggacgag cgtcatgaat cccgagaaga
    10621 cgaccatctg gcaggccccg gcggcgacga ggaggacgag agagaaggtc ggctcgcggc
    10681 agagggcgaa agcgaccatg aggagaccgt agaggacgag cccgagcgcg gaaaggaggc
    10741 cccgccgcgg caggcgagcc gcacccgcga cggcgagccc gccgacgacc gcgccgcacc
    10801 cgaacgcggc gaggagaacg ctgtagcgct ccgcgccggc gtggaagacg tcccgggcga
    10861 aaaccggaag gaatgtcagg agcgggttcg agcagaacgt gccgaggaag gcgagagcga
    10921 cgaggccccg cagcgacggg cgcctccaga ccgtggcgac cccttcgcgc aggccgtcca
    10981 ggacggtcac ccccccgctc ccggtcccgg ggcggcgggg gatcgcggcg agcgccgcca
    11041 cgaccgcgag gaaggagagc ccgttgagcc ccatgcagcc cgcggctccg aaccgcgcga
    11101 aggcgaggcc accgatcgcc ggcccgacga cgcgcgcgaa gttgaactgg atcgagttca
    11161 ggaggatcgc ccggggcagg tcggccggcg ggacgagcat cgggacgagc gcctggaagg
    11221 ccggcccgcc gaacgcctgg gcgagcccga cgacgaccga ggtcgcgagg atcgctccga
    11281 gcgtgatccg gccggtggcg atcagcgtca tcaggacgag cgcggaagcc atctggacga
    11341 cctgggagac gaggaggatc cgccgccgct ccgcccggtc ggcgaggaca cccccgaaga
    11401 gcgaaaaggc gaggaacggg gcctcgccga ggaaggcgtc gaccccgagc cagaaggggg
    11461 agccggtcag ggtcaggacg agccacccct gggcgaccct ctgcatccag gtcccgacgt
    11521 tcgagacgag ggaaccgctc cagaggaggc ggaagtccct gtggcggagc ggcgtgaagg
    11581 ccgcgacgag ggcgggcacc ggcaacgagt ctcgcacgag cggtcgatcc cgacgcgcgc
    11641 cggacccccg ccccgtgcta gcgttggcgg ttgcccgcgg gccgaccggc ccgccgcctg
    11701 aaggaggcct tcgtgctgcg cacacccgtc gtcttcctca ccggagccag cggagagatc
    11761 ggccacacgc tcatcgagcg cctgtcggag gacgggagcc gtcccgtcat cacgctcgac
    11821 ctcaagcccc tcgacccgtc gatcgcgccg cgcgtggcgc ggcacttcac gggctcgatc
    11881 ctcgagccgc agctcctcga gcgaatcctc tccgagttcg aagtcgacca ggtcttccac
    11941 ctcgccgcgc tcctctccac gcggagcgag ttcaccccgg tgaccgcgca ccaggtgaac
    12001 gtcgagggga cgatggcgat gctcgagttc gcccagaagc agggcgagtc gcacgggcgg
    12061 ccggtcgtct tcctgtaccc gtcgtcgatc gccgcctacg gcatgccggg gctcgacgtg
    12121 aaggcgaagg ccgggaaggt gaaggaggac gacttcacga acccgacgac gatgtacggc
    12181 tgcaacaagc tctactgcga gcagctcggc cgctactatg cccggcacta caagcagctc
    12241 gcgccggaga cgctctcggg gaaggtcgac ttccggtcaa tccggttccc cggcctcatc
    12301 tccgcgttca ccgtcccctc gggcggcacg tccgacttcg cgccagagat gatccacgcc
    12361 gccgcgcagg ggaagcccta cgcctgcttc gtgaggcccg acgcgcgcat ccccttcatg
    12421 gcgatgcccg acgccgtaga cgcgctcctg cagctcgccg ccgcccctcg cgagaagctg
    12481 acgcggacga cgtacaacat cggggccttc gccccctcgg ccgccgagat ccaggcgatc
    12541 gtcgagaagg cgttccccgg cgcggagatc acgacgaagg tcgacgcgaa gcgccagggg
    12601 atcgtcgact cgtggcccgc cgacgtcgac gactcggcgg cccggaagga ctggggcctc
    12661 tctccccgct acgacctcga ccgcgccttc caggagtacc tgatcccgaa catccggaag
    12721 gcctaccagg gttgaggccg ggcgagcccg gccttcaccc gccgggctcg cccgcggagg
    12781 ccgccggctc cggcttcacg agccggagcc gcgtcgcctc gtcgacgacg tagaggcgca
    12841 tccggggcag gaccttctcg agggtctcga gatagagccg cgcgcggagg ggcccggggg
    12901 cgaggctcgc ctctcggcgc agcgcgtcga accgcgccgc gtcgccgtga gcctcctcga
    12961 cccgcctctg tctctggctg gaggcggcgg agacgagcgc ctccgcctcg ccgcgcgcgc
    13021 gcggaagcgt gtcgttcgcg tagctctcgg cctcgttcac gtatcgctcc cggtcgctct
    13081 tggcgtcctg cacatcctgg aacgcggcga ggacgggcgg gggcgggtcg acggaacgaa
    13141 gggccacgga gacgacggcg acgccgctct gccaccggtc gaggagctcc tgcgtcccgc
    13201 ggcgcacctc ctcgagcagc gtgtggcggc cgcccgtcag cgcgtcgtcc accggcagga
    13261 cgcccatcgc ctccgtcacg acggactcgg cggcccggcg gacgacgtcg gccgtgtcac
    13321 gcccgtggac gacccattcg accgggcggg cgacgcggta ctgcaccgcg agctgaacgg
    13381 cgaggatgtt cgtgtcgccc gtcagccact gcccttcgtc cgccgggagc gacaccgcgc
    13441 gtcccccgtc ggcggtccgg aagccgatcg gcaccgtctc ggtcttcgtc acctccacct
    13501 tgacgacgcg gtcgattccc gccggccgcc agtggatgcc ggggccgagc gggagcggct
    13561 gaaccgcgcc gagccgcagc acgaccccct gctgcgccgg tcggagggcc gagacgcccg
    13621 cgaggagcca ggccccgaag gcgagccccg aggcggcgaa gagcgcccgt cgcaccgtcc
    13681 gggtcaattc ggtcccttcc tcggcggcgg cggcgcggcg ccgtcggtca ggaggcgcag
    13741 gacgtcggag tcgcccggca ggacgagcgt cgtcttctcg tcgaggatcg attcgtaggc
    13801 ccggagcgtc cggaggaact tgtagagctc cgggtcctgg ttcgtcgccc tcgcgtagat
    13861 ccgagcggcc tccgcctcgc cggccccctt ctcctcctcg gccctccggt acgcctcgga
    13921 gaggagccgc gtcttctccc ggtcggcctc ggcgcggatc ttgagcgccg cctcctcccc
    13981 ttccgaacgg aaccgccggg cgatccgctc ccgctcggac ctcatccgcc cgaagaccga
    14041 gagcaggttc tgctcgggga aggcgatccg cttcagcctc acatcgacga ccttcacgcc
    14101 gtagtcggcg gcggcgcgcg cgcgaacacg ctcgccaatc ccggcgacga cgcccgcgag
    14161 gtcggcctcg ccctccgtcg ccgagacgag gcgggtaaac ggcacggcgc cgagggcggc
    14221 gcccgtttcg gaggcgacga ggtcggcgag gcgcgcctcg gcgctctcgc gcgtgacgag
    14281 cgtctggagg aacttgagcg gctcgtcgat ccgccagagg acgaccgggg cgacgacgat
    14341 gttcttcttg tcgttcgtca ggaactcccc tgccggcggg tcgaagtaga ggaggcgcgc
    14401 gtcgagccgg gcgacgtcgt cgacgagcgg cacccgggcg tggaggcccg gctccgtcac
    14461 gaccgcgaca ggacggccga agcgggtccg caccgcgaac tccgtcgcct cgacgacgta
    14521 cggcacccgc gcgatcagga ggaggaggat cgcgcccgcg gccaccgcgg cgacgcgtcg
    14581 gcgtcgggac cgggccgggg gctcgagggt ctcttccatg gcgttcacct cagcgctccg
    14641 ccgcggggac gcccggaggc ggcgcgggga tcggattcac gaaaccggcg agtcctcgcg
    14701 ccggcgcggg caccgaggcg gcctcgcccg gcagggccgg cgtcacccag agctcgaacc
    14761 cgggggcgcg cctcgggtcg ggacgaatca gcttcggaac gcccgacagg accctctcga
    14821 gcgcctccag cttcagacgc aggcggctcg tccgcggcgc cgcggcgacc tcgcgcgcga
    14881 gggggacgaa cgcggcggcg tcccccttcg cgtgctcgac gaggcgggtc ctctcggacc
    14941 cggcctcggc gagcgtccgg gcgacctctc cgcgcgcctg cccgagagcc ccttccgcct
    15001 cgacgagagc cttgttccgg gcggtcgtcc ggtcctcggc ggccgacgcg atgtcgcgga
    15061 aggcgccgtg gacgtcgtcc ggagcgtgga cgaagaggag ccgcaggtcg accggcgcga
    15121 gccccgtctc ccggaggacc ggcgccttcc ggagcgcctc gaggacgctc ctctccacgg
    15181 cgccccgctc ggcggtgtag atggcgtcga tcggcgtccg accgacctcc tcgacgagaa
    15241 gcgcccgggc gaggtcgcgg aaagccggct cggcgcggtc gatgccgaac cggaacgcgg
    15301 ccgggtcggc gacccggtag tggacgacgg ccgtgacctc gatgacgttc tcgtcgcccg
    15361 tcaggaactg cgcctcctcg gggacccgcg tcgactgcgt cggtacgaag acacgcgacg
    15421 tgacgggcgc cgcggcggag ccggtggccc ggtagcggaa gccgatctcg acggagcgca
    15481 gtcgcgcggt caggatcgcc tccgccttcc cgagcgggaa cggcgggacg aggtggaggc
    15541 ccggggcgag atcggacgcg gtcaccttcc cgagggtccg gacgagccct gtctcgtcgg
    15601 gccggacgac gagaagcgcg gtgcggcccc aggagaggag gaggagcgtc gcggcgagtc
    15661 ccgcgagcgc ccgtcgcggg agacgtcggg gcgcttgctc gcggacgagg tcgcggaacg
    15721 cgcgggcgtg acccttcagc tcgttccacc ccggccggaa cccctgccgc tggaagctga
    15781 aggccatcag cgcgaggaag gcgaaggccg agaggaactt gacgacggcc caggcgcccc
    15841 cgctctccgc ctcggggctg ccgggcgccg gaaccaggcc cgcgccgacg acggcgttca
    15901 gaaggaggcc cgacgcgatc gcgacgccga cgatcgaccc gacgtagatc ccgacgaagc
    15961 gccggccgaa catccgggcg acgaccgcga tcgtcgaggc gttcgtcgcc ggtcccgcca
    16021 gcatgaagac gagcgccgag cccgggtcga ggcccttggc gatcatcgcg gcggcgatgg
    16081 gcgtcgaggc gctcgcgcag acgtacatcg ggagcccgac gagggccatc aggaccatcg
    16141 tcaggagcgg ccacgggagg aaccggagga agaagtcttc cggcaggacg cccgccagca
    16201 ctcccgtcgc gaggaacgcc aggagcatcc agaacgcgag ctcgtcgacc atcgtgacga
    16261 acgcgtaccg gaagacccgc gcggcccgct cccgcagagt cccgcttcgc gcggcggggg
    16321 tctcggtcgc cccgcagccg tcctcggtcg agcagggaac cgccgggccg tcggccgcct
    16381 cacccgcctc cgtggaacgt cgatcgtctt ccccgcctgc gacgagcgag acgagcccgg
    16441 ccacgatcgc ggtcacgacg ccgacgacgg gacggacgag agccatgacg gggccaagaa
    16501 gcccccacgt caggaggatc gcctcctcgc tcgtctccgg cgtcgagatc aggaacgaga
    16561 cggtggcctc gcggctcgcc cccttcttcc gaagggcgac cgccatcggc agcaccccgc
    16621 aggagcagag cggcagcggt ccgccgatga ccgccgcctt caggatcgcc ccgaggctcc
    16681 tcccgccgag ggcgcgtccg atccgggccg tgtcgacgag ctcgtggaga aggcccgcga
    16741 ggaggaaccc gaggaggatg aagaagccgc tgtcgaggac gatccgatag gtctcctcga
    16801 agatccggac gaggaggctc acggggctcc gatgcgccgg ccgcggtccg ttttcacgac
    16861 gggagtatcg cccctccccg cacgccggcg aagggacgcg ccccggccgc acccgcaagg
    16921 ccgattcccc tcgtccgccg ccgaccgccg tgacttctgt tcgcgaatga cgcgccgcgc
    16981 atgaccggcg tcatac
//
LOCUS       DAOONL010000074        16787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3598_length_16787_cov_19.560602, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000074 DAOONL010000000
VERSION     DAOONL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3598_length_16787_cov_19.560602"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..2454
                     /locus_tag="PLP50_11395"
     CDS             94..2454
                     /locus_tag="PLP50_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52196.1"
                     /translation="MALRGRVREALAGAAPAALLVAAFATLFFDVLVLGQTFLGSDPA
                     RHHFPLLSLVRPLWRLEGGLPLWNPLLAAGQPWAANPEQAAFHPLTWLFLVLPPTLAF
                     RLQVLVSVAVAMAGAAFLGRALRLPPAAATVLALAWGTGGAFLSSTQFFPTLFAGAAL
                     PWVLGFAARVASGGGPSAVAGLAVAEALLCAAGEPTQLLLSGPLAILTAWHALRACHE
                     ADAPSRAQRRVAARTAHLAAGLALGALIAAPTLLPGALHARKTVRAEGLPEHVASYWS
                     ATPWRAGELFVPSVLGHDAVPAVGGAARDAWRDRLYPGRGEPYLSSVYPGLVATLLAA
                     FAWGRGVPGARAWGSAGLLAALVAAGSHGPLWAHLRKLPLLGGTRYPEKLLLAAAFAV
                     AVSAALGLAALAEAPARARRGLGMSAAAGALLLLAGWGLGATSSEAYPAQLLTGQALP
                     QAALAASAAALLLLVPETERGRTALGGLLLVLLGADLVLRGRPLLRTGAPRHLVELPP
                     QFEALRRNPPRGYLVHAAAWTPELWKVEAIGEPPAMARFGIASALDYDFGMTELAWSA
                     RATETIFSTIARAPDAAGPVLRRRSVAGVIRFRPGTPRSRKEADRIPRAELLELLALR
                     DPLPFAFLATEARLVHDAADWSRTVVALGEAAATTACIDAAHAGPLPPRFSGGGAAVL
                     ERRPGRIRIEVTAEGPEPALLAVNQTWDPGWRATVDGRLVPLLLTDLSLSGLVVPPGR
                     HEVVLRYEDPAVKAGLALGAAGLVACSALALRGRRRLRTGAVSSKG"
     gene            2451..3224
                     /locus_tag="PLP50_11400"
     CDS             2451..3224
                     /locus_tag="PLP50_11400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA52197.1"
                     /translation="MSTSDDRDPWYASWFGEEYLALYPSRDDLEAARQAQFAFGLLAP
                     YAPKASGGILDLACGTGRHAVVLSRGGFPVTGVDLSAPLLSRARARRAEPDPGYVRAD
                     MRRLPFGSGSFGAVVNFFTSFGYFDDPSDDLRVVGEVGRVLATGGAFLSDVFNATRVL
                     ATLVSHEEKTVAGERVSIRRRYDAATRRLEKEITMRSGSTARTFRERVRVYLRDELES
                     IHRSAGLRVVAAFGDFDGSPFDAKRSPRLILLTVSGGAR"
     gene            3221..4783
                     /gene="bshC"
                     /locus_tag="PLP50_11405"
     CDS             3221..4783
                     /gene="bshC"
                     /locus_tag="PLP50_11405"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HPA52198.1"
                     /translation="MTVPLGTLPGIPLLARGHATGDPDVSRLLGERASVDEIAARARE
                     VLRVFRPRAGADAPLEALAAGKRAAVLTGQQVGLLTGPLLTAVKALAAVKVAEDVTAA
                     GTPLAPVFWCASEDHDLVEVTRVPLPGPDGPVEAGPEAEPLARNRRPVGGLPVEVDVA
                     AILERARAEAGALADEESFEALRAAHAGATYRNAFVASLSWLLGEPALLFADAARAEE
                     KPVLVPLAVRLVREREAVRRLLEARAAELAAAGHALQVVSEAGALPLFAIIDGERLLL
                     REREGALELKGRDGERFAPEEVVARLESGEWLPSFSALSRPLAASTLYPVAATVLGPA
                     ELAYWAQMRPLFDWAGLVPPVLVPRPMAAPVTAPERKVLGRLGLTLADLFAGVDALLA
                     ERGAARAGGLLDELVALRDGALSGLDALAPRLAAVEAGLGKNVVATRENVRFAFEKLA
                     DRARAAAGRADEAEVRQLRRLAASLAPGGRPAERLYPPLPWLLRHGRAGLVGPLKEQL
                     RWDVAGLQEIDL"
     gene            4780..5508
                     /gene="bshB1"
                     /locus_tag="PLP50_11410"
     CDS             4780..5508
                     /gene="bshB1"
                     /locus_tag="PLP50_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HPA52199.1"
                     /translation="MTETVEILAVGAHPDDVELGCGGTLALLARAGRSVGILDLTRGE
                     AGTRGTPETRAAEAAEAARLIGARFRDGLDLGDGNLRTDRAAELEVIEVVRRRRPRLV
                     FAPLPNDRHPDHVRAGRLVADAAFYAGLRALETGLPPHRPQQVVYFPSTFLAEPTFLV
                     DVTETLEVKLAAVRAFRSQFFDPASKEPSTFISSPEFLDGVTARARAFGRLANVGAAE
                     GFVSPRPPLVSDPLAAFDGFEKGF"
     gene            5512..6642
                     /gene="bshA"
                     /locus_tag="PLP50_11415"
     CDS             5512..6642
                     /gene="bshA"
                     /locus_tag="PLP50_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HPA52200.1"
                     /translation="MKIGIACYPTFGGSGVVATELGHELASRGHDVHFVSYALPSRLN
                     VFRRGVSYHEVAVPTYPLFNYPPYDLALATRIVDVALHEGLDVLHAHYALPHAISAHL
                     AREMLLPRRLAVVTTLHGTDVTIVGQDRSYLPITRFGIERSDAVTAVSEYLARVTREV
                     FEPVKEIEVVPNFIDPERWRPADGPRRRSCFAKAGEKLLLHVSNFRPVKRVLDVVEVF
                     DLVRREVPARLLMIGDGPDRPAAEELCRARGIAGLVHFLGNTPAVEEVMPTADLYVLT
                     TDMESFGLSALEAQASGVPVLGYRAGGLPEVVESGTTGFLRPVGDVAGLAADGVALLG
                     DDLTYREASSAARARAVALFDADTIVERYLALYERVVAEAGG"
     gene            complement(6609..7610)
                     /gene="add"
                     /locus_tag="PLP50_11420"
     CDS             complement(6609..7610)
                     /gene="add"
                     /locus_tag="PLP50_11420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPA52201.1"
                     /translation="MAVADALRALALRPKAELHLHLEGAVRPATLVGLARRAGSPLFH
                     DLAAVRARSRPGSLAGFLTLYRDVCRLLRGPDDFALLARDLTARLRRGRIVYAEVYVS
                     PAVVERVGLPWPPVRDAVERVFAAHERAGHGRVRVLLDAVRHFGPDAAHRVLDLEASH
                     PWPRAVGFGLGGDELALPARDFVSVYRRLPGLGLAPVVHAGETGGPDSVAEALRWLRP
                     RRIAHGVGAAADPALVRRLARSGVALDVCLTSNAATGTHPLGGHPVLRLLRSGVAVTL
                     ATDDPGLFGTTLLGEYRLLARLGATERELASVLRAGFRAALSPAGRHQPPASATTLS"
     gene            7548..8060
                     /locus_tag="PLP50_11425"
     CDS             7548..8060
                     /locus_tag="PLP50_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPA52202.1"
                     /translation="MEVELGLRAEGEGAEGVGDGHWAASYGRRRPAEPTAVPSERLWL
                     RAAALGLDLICLAGGPLLLATVVVFLIGVFAPDPPAGLPRVFRAAQALFVLLFLLRDV
                     RGASPGKALLGLAVVRTDRGPLRVRDSILRNLPLLVPGLNLLEALAVLRRADSRRLGD
                     RLAGTAVGES"
     gene            8113..8931
                     /locus_tag="PLP50_11430"
     CDS             8113..8931
                     /locus_tag="PLP50_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPA52203.1"
                     /translation="MDRIPRLPRLIRPLAALAVATLALTGGALAGQVLEEPFEKTYDG
                     NGIERVRLQNVNGPVRVGTWDKAHIRVSAVKRAKGSRAEEAIAETEIRVAKRAGAIEI
                     ETVLPKPTRSWGIFSWGSRGGAEVAYELLLPAALPVDVETVNGRVTAEKRLGSLTLNT
                     VNGSVRVEEQGGPLHVNTVNGSVEVVFSGPLQAADLETVNGSVTVTCARDSSFRFDLQ
                     TVNGKIRSDFPDVTVEGKWGPKEARGEIAGGRERLTVETVNGEVRVLAGDAAER"
     gene            8979..9350
                     /locus_tag="PLP50_11435"
     CDS             8979..9350
                     /locus_tag="PLP50_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52204.1"
                     /translation="MPEEVGAPAPPPPPPPPPAPPASSNRSVMLVLSYLGILALIPLL
                     TEKNDREVQWHAKHGLVLLVAYLVLMVGIMIASFVIGFFGMLQIPLWLGYLVVIILGI
                     TNALNGKRFVIPGVSEFAEKL"
     gene            complement(9431..9515)
                     /locus_tag="PLP50_11440"
     tRNA            complement(9431..9515)
                     /locus_tag="PLP50_11440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9479..9481),aa:Leu,seq:gag)
     gene            complement(9585..9998)
                     /gene="secG"
                     /locus_tag="PLP50_11445"
     CDS             complement(9585..9998)
                     /gene="secG"
                     /locus_tag="PLP50_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPA52205.1"
                     /translation="MIYVLVPIYVIVCVFLILVVLLQQGKGADVASALGASSSQTTFG
                     ARGATTVLEKVTTWSFVAFSLLAVSISLLQARPKSSVIKQLGSQPVKSAPAAPAAPAP
                     ANAVPVAPAPGGAVPVAPAPANAEPAPAAPAPAPN"
     gene            complement(10096..10863)
                     /gene="tpiA"
                     /locus_tag="PLP50_11450"
     CDS             complement(10096..10863)
                     /gene="tpiA"
                     /locus_tag="PLP50_11450"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPA52206.1"
                     /translation="MHPSRTPLVIANWKMNKTPSEAAVFCDDFLSRLPALPERVELAI
                     APAFPALERVGRHLLPTRVRLAAQDVHVEPKGAHTGEVSVAMLADVGVAMVLVGHSER
                     RRDRKEAEADFTRKMKRLADAGLSPVYCVGETLEERDGGRTEAVLAAQMTALDAFGDA
                     LPPGFVLAYEPVWAIGTGRAATPEMAADAHAFLRARLSERFGPAAAVATRILYGGSVT
                     PANAKELFGKEDIDGGLVGGASLVPADFAALAVAAGA"
     gene            complement(10926..12116)
                     /locus_tag="PLP50_11455"
     CDS             complement(10926..12116)
                     /locus_tag="PLP50_11455"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPA52207.1"
                     /translation="MATLPRSIRDLELSSRRVFLRVDFNVPIKEGVVKDDTRITEALP
                     TIRLAREKGARLILASHLGKAKGTPDPKYSLSPVAKKLEALLGAPVAFAGDCVGEAAE
                     GAAKALADGGVLLLENTRFRAGEEGNDPAFAKALASLAEVYVNDAFGTAHRAHASTAG
                     MAAHFGPGARGVGLLMEKELNALGKVVDDIDRPFVGILGGAKILGKIAALEALVERAD
                     TLLVGGGMANHFVVALGLGIGKSLLEPEGVPIARKVIDRCKERGVNLVLPSDFLVAPS
                     LEAAGQAKAVAMNAIPADQAALDVGPKTLEMFARMAKGAKTIFWNGPMGVFETKPFDA
                     GTRGVAKLMAESGAFTVVGGGESVEAINEAGLGPKISHVSTGGGASLDLISGKKLPGV
                     EALL"
     gene            12202..13299
                     /gene="mtnA"
                     /locus_tag="PLP50_11460"
     CDS             12202..13299
                     /gene="mtnA"
                     /locus_tag="PLP50_11460"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPA52208.1"
                     /translation="MSETPLGTRVRPIDTASGRLRLLDQRRLPREETWIDCDSADATA
                     EAIRSLAVRGAPLIGVAAAWGLAVEARRLAGADGGFDASWEAAADRLGKARPTAVNLR
                     FGVERMRTAFRASAGRPPAERVALLEAEAARFEEEDREACAAMGRHGAAWIAAHLGAD
                     PAGPLRVLTHCNAGALATTGVGTALGVVRALAAGRPVAVFADETRPFWQGARLTAWEL
                     AKDGIDVTILPDVAAASVIASGKVSVAVVGADRIAANGDVANKVGTYGVALACRAHGV
                     PFVVAAPRTTLDSACPTGRDIPIEWRDGAEVLLLDPAGESPLSVAPAGVPALYPAFDV
                     TPAALVDAIVTEAGVSTAPHADGLARLLALP"
     gene            13296..14402
                     /gene="tsaD"
                     /locus_tag="PLP50_11465"
     CDS             13296..14402
                     /gene="tsaD"
                     /locus_tag="PLP50_11465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPA52209.1"
                     /translation="MTYVLGLETSCDETSVALLREDGSIAASVVSSQIDMHKRYGGVV
                     PEIAARAHLTNLPPLLDEALEQAGAHLQEVGLVVATAGPGLVGALLVGLAEGRGLALG
                     LGVPFVPGHHIEGHALSPFLDLDGGPARAVPPRFAALVVSGGHSHLFSFAPKGISVLA
                     KTRDDAAGEAYDKVAKMAGLGYPGGPVVDRLARSGRVVEPFAVPRFKGGSHDFSFSGL
                     KTAVRDRLATLGFAPNTGVALRGPALPEEEAPPALRDLLAAFQEAAVAQLEDRVERLH
                     ESERFPLLTVSGGVAANSLLRERLAAWGTRRGVEVLLAPRALTSDNAAMVAFAGLLRH
                     RAGAPREGLSAPARSRWPLEGLPWGASAGPPRFR"
     gene            14438..16237
                     /gene="lepA"
                     /locus_tag="PLP50_11470"
     CDS             14438..16237
                     /gene="lepA"
                     /locus_tag="PLP50_11470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPA52210.1"
                     /translation="MPDPSRIRNFSIVAHIDHGKTTLSDRILEITGALSAREMREQVL
                     DDNPIEQERGITIKARAVSLKYRSEDGQDWVLNLIDTPGHVDFTYEVSRSLAACEGAV
                     LVVDATQGVEAQTLANAYLAIHGDLTIVPCINKVDLPSAVPEMAREQIEEVIGIPAQH
                     AALTSGKVGTGVRDLLEHIVHDVPAPTGDPNGPLRALLFDSWFDVYRGVTCLVRVKDG
                     AIRPGMKIKMLGMDLVSEVQEVGVFTPKAQVSPELTVGMVGYVIAGIKDLHSTKIGDT
                     MTEAERPASEPLPGFQDVKPMVFASIFPTESDDYEDLRDAMEKLRLNDASFTFEPESS
                     TALGFGFRCGYLGLLHLEIVQERLEREFDLSLITTAPSVPYRVTTTRGEVLEISNPVK
                     LPETQLIDVIEEPRILATIITKDEYLGGILALCEERRGRQVSLEYAGTQRAVLKYDLP
                     LNEVILDFYDKLKSVSRGYASFDYQLTGYAEADLVKLDVLINSEPVDALAFIVHRDKA
                     QAKGRALVEKMKEMIPQQMFEVIIQAAIGAKILARSTVKALRKNVLAKCYGGDITRKR
                     KLLEKQKEGKKRMKKVGRVELPQEAFLAVLKVD"
     gene            16249..>16787
                     /gene="lepB"
                     /locus_tag="PLP50_11475"
     CDS             16249..>16787
                     /gene="lepB"
                     /locus_tag="PLP50_11475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPA52211.1"
                     /translation="MASSAGDGPAPRVPRHRTTLREYVEAVLVAVVFALFAKAFLVET
                     YQIPSGSMEESLLVGDHVVVDKLGWAPRGLPWGPLLPSRGLRRGDVVVFRGPEEPGRD
                     FIKRAVALPGETLEIRGKRLFVEGVAQAEPWVVHRDPAVLSGEGLPASVRGRDELAPR
                     TIPAETFFALGDNRDESRDS"
BASE COUNT         2135 a         6299 c         6013 g         2340 t
ORIGIN      
        1 gcacccgagc ctcgccgccg tcatcgacga gctcgcgctc tgggggttct gagccccgag
       61 cgtcgccccc cgcgcctcgg atagcatcga cggatggcgc ttcgtgggcg ggtgagggag
      121 gctctcgcgg gagcggctcc ggccgcgctc ctcgtcgccg ccttcgccac actcttcttc
      181 gacgtcctcg tcctggggca gacgttcctc ggaagtgacc cggcccgcca ccactttccc
      241 ctgctgagcc tcgtccggcc gctctggagg ctcgaggggg ggctccccct ctggaacccc
      301 ctcctcgccg cggggcagcc gtgggccgcg aaccccgaac aggcggcttt ccaccccctt
      361 acgtggctct tcctcgtcct cccgcccacg ctcgccttcc gcctgcaggt cctcgtttcc
      421 gtcgcggttg cgatggcggg cgcggcgttc ctgggccggg cgctccgcct gccgcccgcg
      481 gcagccacgg tcctcgccct ggcctggggg acgggcggcg cgtttctctc gtcgacccag
      541 ttcttcccga ccctcttcgc gggcgcggcc ctcccctggg tcctcggttt cgccgcccgg
      601 gtggcgagcg gcgggggacc gtccgccgtc gccggcctcg ccgtcgccga agccctgctc
      661 tgcgcggccg gcgagccgac ccagctgctc ctctcgggac cgctcgcgat cctcaccgcg
      721 tggcacgcgc tccgagcgtg ccacgaagcc gatgcgccgt cccgtgcaca gcggcgcgtc
      781 gcggcgagga cggcgcacct cgcggccggc ctcgccctcg gtgccctgat cgcggcgccg
      841 acgctcctcc cgggcgcgct tcacgcacgc aagacggtcc gggccgaggg gcttccggag
      901 cacgtggcgt cctactggtc ggcgacgccc tggagagcgg gggagctctt cgtcccgagc
      961 gtcctcggtc acgatgccgt tccggccgtt ggcggagctg ctcgcgacgc atggcgggac
     1021 cggctctacc ccggacgcgg cgagccgtac ctctcctccg tctacccggg cctcgtcgcg
     1081 accctcctcg ccgccttcgc ctgggggcgc ggcgtccccg gagcccgagc ctggggatcc
     1141 gccggccttc tcgccgcgct ggtggccgcg ggcagccacg gaccgctctg ggcgcacctt
     1201 cggaagcttc ctctgctggg cggcacccgt tacccggaga agcttctcct cgccgccgcg
     1261 ttcgcggtgg cggtctcggc agcgctcggc ctcgccgccc tggccgaggc gccggcgcgc
     1321 gcgaggcgcg gcctcggcat gtccgccgcc gccggcgccc tcctgctcct cgccggatgg
     1381 gggctcggcg cgacctcttc cgaagcgtac ccggcgcagc tcctcacggg gcaggcgctg
     1441 ccgcaggcgg cgctcgcggc ctcggccgcc gcgcttcttc tcctggtccc ggagaccgaa
     1501 agaggacgta cggcgctcgg cggcctgctc ctcgtgctcc tcggcgccga cctcgtcctt
     1561 agggggcgac cgcttctccg gacgggagcc cctcggcatc tcgtagagct cccgcctcag
     1621 ttcgaggcgc tccggcggaa tccgccgcgc ggctacctcg tccacgcggc cgcctggacg
     1681 cccgagctct ggaaggtcga ggcgatcggc gagccgcccg cgatggcgcg cttcgggatc
     1741 gcgtcggcgc tcgactacga cttcgggatg acggagctcg cctggagcgc ccgcgcgacg
     1801 gagacgatct tctccacgat cgcgcgcgcg cccgatgcgg cgggcccggt cctgcggcgg
     1861 agatcggtcg caggcgtaat ccgctttcgg ccgggaacgc cgcgctcgcg gaaggaggcc
     1921 gaccgcatcc cgcgagcgga gctcctcgag ctcctcgcgc tccgcgaccc gctcccgttc
     1981 gcgttccttg cgacggaggc ccgcctcgtg cacgacgcgg cggattggag ccgaaccgtc
     2041 gtcgcgctcg gcgaggcggc cgcgacgacc gcgtgcatcg acgcggcgca cgccggaccg
     2101 ctgccgcccc gcttctcggg aggcggggcc gccgtcctcg agcgccggcc cgggcggatc
     2161 cggatcgagg tcaccgccga ggggccggag ccggcccttc tcgccgtgaa ccagacgtgg
     2221 gaccccggct ggcgggcgac cgtcgatggt cgtctggtgc cgctcctgct gacggatctc
     2281 tcgctctcgg gcctcgtcgt ccctccgggt cggcacgagg tcgtcctccg gtacgaggac
     2341 ccggccgtga aggccggcct cgcgctcggc gccgccggcc tcgtcgcctg ttccgcgctc
     2401 gccctgcggg gccggcggag gctccggacg ggggccgtat catcgaaggg ttgagcacct
     2461 ccgacgacag ggacccctgg tacgcgagct ggttcggaga ggagtacctc gccctctacc
     2521 cgagccggga tgacctggag gcggcgcgcc aggcacagtt cgccttcggc ctcctcgcgc
     2581 cgtacgcgcc gaaagcgagc ggcgggatcc tcgacctcgc ctgcggaacg ggacgccacg
     2641 ccgtcgtcct ctcccggggc ggcttcccgg tgacgggcgt cgacctctcc gcgccgctcc
     2701 tctcccgggc gcgggcccgg cgggccgagc cggaccccgg gtacgtccgc gccgacatgc
     2761 gccgtctccc gttcgggagc gggagcttcg gcgccgtcgt caacttcttc acctcgttcg
     2821 ggtacttcga cgacccgtcc gacgacctcc gcgtcgtcgg cgaggtggga cgggtgctcg
     2881 cgacgggcgg cgcgttcctc tccgacgtct tcaacgcgac ccgcgtcctc gcgacgctgg
     2941 tctcgcacga ggagaagacg gtggccgggg agagggtctc gatccgacga cggtacgacg
     3001 cggcgacgcg gcggctcgag aaggagatca cgatgcgcag cggctctacg gcgcgcacgt
     3061 tccgggaaag ggtgcgagtc tacctccgcg acgagctcga gtcgatccac cggagcgccg
     3121 gcctgcgggt cgtcgccgcc ttcggcgact tcgacggctc cccgttcgac gcgaagcgct
     3181 cgccccgcct gatcctcctc accgtgtccg gaggcgcgcg gtgacggtcc cgctcgggac
     3241 gctccccggg attccgctcc tcgcccgggg tcacgccacc ggcgatcccg acgtctcacg
     3301 cctcctcggc gagagggctt cggtcgacga gatcgccgcg cgggcgaggg aggtcctccg
     3361 cgtgttccgg ccccgggcgg gtgccgacgc cccgctcgag gcgctcgccg ccggcaagcg
     3421 ggccgcggtc ctcacgggcc agcaggtcgg cctcctcacc gggccgctcc tgaccgcggt
     3481 taaggcgctc gcggcggtga aggtggcgga ggacgtgacg gcggcgggga cgccgctcgc
     3541 cccggtcttc tggtgcgcgt ccgaggacca cgacctcgtc gaggtgacgc gcgtccctct
     3601 cccgggaccg gacggtcccg tcgaggcggg gcccgaggcc gagcctctcg cgcgcaaccg
     3661 ccgccccgtc ggcgggctcc ccgtcgaggt cgacgtcgcg gcgatcctgg aacgggcgcg
     3721 cgccgaagcg ggagcgctcg ccgacgagga gagcttcgag gccctgcgcg cggcgcacgc
     3781 gggggcgacc tatcggaacg cgttcgtggc ttcgctgtcg tggctcctcg gcgagccggc
     3841 gctcctcttc gcggacgcgg cgcgcgctga ggagaagccg gtcctcgtcc cgctcgccgt
     3901 ccgcctcgtg agggagcggg aggccgtgcg gcgcctcctc gaggcgcgcg cggcggagct
     3961 ggccgccgcg gggcacgctc tccaggtcgt ctcggaggcc ggggccctcc cgctcttcgc
     4021 gatcatcgac ggggagcggc tcctcctccg cgagcgggag ggggccctcg agctgaaggg
     4081 ccgcgacggc gagcgcttcg cgccggaaga ggtcgtcgcc cgcctcgagt cgggcgagtg
     4141 gctcccctcc ttctccgcgc tctcgcgccc cctcgccgcg tcgacgcttt accccgtcgc
     4201 cgcgaccgtc ctcgggccgg cggagctcgc ctactgggcg cagatgcggc cgctcttcga
     4261 ctgggccggg ctcgtcccgc ccgtcctcgt cccgcggccg atggccgcac ccgtcacggc
     4321 tccggagcgc aaggtcctcg gccggctcgg gctcactctc gccgacctct tcgccggcgt
     4381 cgacgccctc ctcgcggagc gcggcgcggc ccgcgccggg ggactcctcg acgagctcgt
     4441 cgccctgcgc gacggagcgc tctcgggcct cgacgctctc gcgccgcggc ttgccgctgt
     4501 cgaagcgggt ctcggaaaga acgtcgtcgc gacgcgcgag aacgtccggt tcgcgttcga
     4561 gaagctcgcc gaccgggccc gggccgccgc cgggcgggcg gacgaggccg aggtgcgcca
     4621 gctccgccgc ctcgcggcgt cgctcgcgcc gggcgggcgt ccggccgaac ggctctatcc
     4681 cccgctgccg tggctcctcc gccacggacg agccggcctc gtcggcccgc tgaaggagca
     4741 gcttcgctgg gacgtcgcgg ggctgcagga gatcgacctg tgaccgagac cgtggagatc
     4801 ctggccgtcg gggcccatcc cgacgacgtc gagctgggct gcggggggac gctcgccctc
     4861 ctcgcccgcg ccggccggag cgtcggcatc ctcgacctga cgcgcggcga ggcggggacg
     4921 cggggcacgc ccgagacgcg cgcggccgag gccgcggaag cggcccgcct gatcggcgcc
     4981 cgcttccggg acggcctcga cctcggcgac gggaacctcc ggacggaccg ggccgccgag
     5041 ctcgaggtga tcgaggtcgt ccgccgcagg cggccacgcc tcgtcttcgc gcccctcccg
     5101 aacgaccggc accccgacca cgtgcgggcc ggccgcctcg tggccgacgc cgcgttctac
     5161 gccggcctgc gcgcgctcga gacggggctc ccgccccacc gcccccagca ggtcgtctac
     5221 ttcccgtcga cgtttctcgc ggagccgacg ttcctggtcg acgtgacgga gaccctggag
     5281 gtgaagctcg ccgcggtccg tgcgttccgg agccagttct tcgacccggc gtcgaaagag
     5341 ccctcgacct tcatctcctc ccccgagttc ctcgacggcg tgaccgcccg cgcgcgggcg
     5401 ttcgggcggc tcgcgaatgt cggggcggcc gaggggttcg tctcgccgcg accgcccctt
     5461 gtttccgatc cgctcgccgc gttcgacggc ttcgagaagg ggttctagcc gatgaagatc
     5521 gggatcgcct gctacccgac cttcggcggc tccggcgtcg tcgcgacgga gctggggcac
     5581 gagctcgcga gccgcgggca cgacgtccac ttcgtcagct acgccctccc gtcgcgcctg
     5641 aacgtcttcc ggcgcggcgt ctcgtaccac gaggtggcgg tccccaccta cccgctcttc
     5701 aactatccgc cgtacgacct cgcgctcgcc acgcgaatcg tcgacgtggc gctccacgag
     5761 gggctcgacg tcctccacgc gcactacgcg ctcccgcacg cgatctcggc acacctcgcg
     5821 cgggagatgc tcctcccgcg gcgtctcgcg gtcgtgacga cgctccacgg gaccgacgtc
     5881 accatcgtcg ggcaggaccg ctcctatctc ccgatcaccc gcttcgggat cgagcgctcc
     5941 gacgccgtca cggccgtctc cgagtacctc gcccgggtca cccgcgaggt cttcgagccg
     6001 gtcaaggaga tcgaggtcgt cccgaacttc atcgaccccg agcgctggcg gccggccgac
     6061 ggcccgcgcc ggcggagctg cttcgcgaaa gcaggcgaga agctcctcct tcacgtctcg
     6121 aacttccggc ccgtgaaaag ggtcctcgac gtcgtcgagg tcttcgacct cgttcgccgc
     6181 gaggttccgg cccggctcct gatgatcggc gacggcccgg accgccccgc cgccgaggag
     6241 ctctgccggg cgcgcgggat cgccgggctc gtccacttcc tcggaaacac gccggcggtc
     6301 gaggaagtga tgccgacggc ggacctctac gtcctgacga ccgacatgga gtcgttcggc
     6361 ctctccgcgc tcgaggccca ggcgagcggc gtgccggtcc tcggctaccg cgccggcggc
     6421 ctgccggagg tcgtcgagag cggcacgacc ggcttcctcc gtccggtcgg agacgtcgcg
     6481 ggcctcgccg ccgacggcgt cgcgctcctc ggagacgacc tcacctaccg cgaggcgtcg
     6541 agcgccgccc gtgcccgtgc cgtggccctg ttcgacgccg acacgatcgt cgaacgctat
     6601 ctcgccctct acgagagggt cgtcgccgaa gccgggggct gatggcggcc ggccggcgaa
     6661 agcgccgccc gaaagccggc gcgaaggacg gaggccagct cccgctcggt cgccccgagg
     6721 cgggcgagga gccggtactc gccgaggagc gtcgttccga agaggcccgg gtcgtcggtc
     6781 gcgagagtca cggcgacgcc ggatcggagc agccgaagga ccggatgtcc cccgagcggg
     6841 tgggtgccgg tcgccgcgtt cgaggtgagg cagacgtcca gggcgacgcc cgaccgggcg
     6901 aggcgccgga cgagggccgg gtcggcggcg gcaccgacgc catgcgcgat ccggcgcggc
     6961 ctcagccagc ggagggcctc ggcgacggag tcggggcctc cggtctcgcc cgcgtgaacg
     7021 acgggcgcga gaccgaggcc gggcaggcgg cggtagacgg agacgaagtc ccgggccggc
     7081 agggcgagct cgtcgccgcc gaggccgaag ccgacggcgc gcggccaggg gtggctcgcc
     7141 tccaggtcca ggacgcggtg agccgcgtcg gggccgaagt gacggaccgc gtcgagcagg
     7201 acgcgaacgc ggccgtgacc ggcccgctcg tgggcggcga agacccgctc gaccgcgtcg
     7261 cggacggggg gccagggaag tccgacgcgc tcgacgacgg ccggcgagac gtagacctcg
     7321 gcgtagacga tccggccgcg gcgaagccgg gccgtcaggt cccgagcgag gagggcgaaa
     7381 tcgtccggtc cgcgcaggag gcggcagacg tcgcggtaga gggtgaggaa gcccgcgagc
     7441 gagccgggtc ggctgcgggc gcggacggcc gcgaggtcgt ggaagagggg cgagccggcc
     7501 cggcgggcga ggccgacgag cgtcgcgggc cggaccgccc cttcgagatg gaggtggagc
     7561 tcggccttcg ggcggagggc gagggcgcgg agggcgtcgg cgacggccac tgggccgcat
     7621 cctacgggag gaggcggccg gcggagccga cggcggtccc gtccgagcgc ctttggctgc
     7681 gggcggcggc gctcggcctc gacttgatct gcctcgccgg cggcccgctc ctcctcgcga
     7741 ccgtcgtcgt cttcctcatc ggggtgttcg ccccggaccc tccggccggc ctcccccggg
     7801 tctttcgggc ggcgcaggcg ctcttcgtcc tcctcttcct cctccgcgac gtccgaggcg
     7861 cgagccccgg gaaggcgttg ctggggctcg cggtcgtccg gaccgaccgg ggcccgctgc
     7921 gggtccgcga ttcgatcctc cggaacctcc ctctcctcgt tccgggtctc aacctgctcg
     7981 aggccctcgc ggtcctccgc cgggccgatt cccggcgcct cggcgaccgc ctggccggca
     8041 ccgcggtcgg ggaatcgtga ccggaccgaa acttccggcc cgtttcgttc gttactgctg
     8101 gtggaggtcg tcatggaccg catccccaga ctccctcgcc tcattcgccc gctcgcggcc
     8161 ctcgccgttg cgaccctcgc cctgacgggc ggggcgctcg ccggccaggt cctcgaagag
     8221 ccgttcgaga agacctatga cggaaacggc atcgaacgcg tccgccttca gaacgtgaac
     8281 gggccggtcc gggtcgggac ctgggacaag gcccacattc gggtctcggc cgtgaagcgg
     8341 gcgaaggggt cccgggccga ggaggcgatc gcggaaaccg agatccgcgt cgcgaagcgg
     8401 gcgggcgcga tcgagatcga gacggtcctc ccgaagccga cgcgatcctg ggggatcttc
     8461 agctggggaa gccggggcgg ggccgaggtc gcctacgagc tcctcctccc ggccgcgcta
     8521 ccggtcgacg tcgagaccgt caacggacgg gtcacggccg aaaagcggct cggatcgctc
     8581 acgctgaaca cggtgaacgg ctctgttcgc gtcgaggagc agggggggcc gctgcacgtc
     8641 aacacggtga acggctccgt ggaggtcgtc ttctccggtc cgctccaggc ggccgacctc
     8701 gagacggtga acggctccgt gaccgtcacc tgcgcgcgcg actcttcctt tcggttcgac
     8761 ctccagacgg tcaacggaaa gatccgatcc gactttcccg acgtgaccgt cgaagggaag
     8821 tggggcccga aggaagcccg cggcgagatc gccgggggcc gcgagcggct gaccgtcgag
     8881 acggtcaacg gcgaggtccg tgtcctcgcg ggagatgcgg ccgagcgctg agcgagcgga
     8941 ttcccgtaca ctccggccat caccgaaagg agatcccgat gccggaagaa gtcggagctc
     9001 cggctccgcc gcccccgccg ccgccgccgc ccgccccgcc ggcgagctcg aacaggtccg
     9061 tgatgctcgt cctctcgtac ctcgggatcc tggcactgat cccgctcctc acggagaaga
     9121 acgaccgcga ggtgcagtgg cacgccaagc acggcctcgt gctcctcgtc gcctacctcg
     9181 tcctgatggt cggcatcatg atcgcgagct tcgtcatcgg cttcttcggg atgctccaga
     9241 tcccgctctg gctcggctat ctcgtcgtca tcatcctcgg catcacgaac gcgctgaacg
     9301 gcaagcggtt cgtcatcccg ggggtgtcgg agttcgccga gaagctctga gcggatcgga
     9361 ccgcgaagcc cggggagccc gggaaagatc gaaggcccgc cttccggcgg gccttcgcgt
     9421 tcctgagaag tggtgccctg ggccagactc gaactggcac ggattgctcc acacgcccct
     9481 caaacgtgcg cgtctaccaa ttccgccacc agggcacggt gggggaggga gatgataccg
     9541 atccgcgccg gggaatcaag cctcgaagcg gatcgggccg ggaatcagtt cggagcgggg
     9601 gccggagccg cgggggccgg ctcggcgttc gccggggcgg gcgcgacggg cacggcgccc
     9661 cccggagcgg gagcgacggg cacggcattc gccggggcgg gggccgcagg ggcggccgga
     9721 gcgctcttga cgggctggct tccgagctgc ttgatgacgg acgatttcgg ccgggcctgc
     9781 agcagggaga tcgagaccgc caggagcgag aacgcgacga acgaccaggt cgtcaccttc
     9841 tcgaggacgg tcgtcgcgcc gcgcgcgccg aaggtcgtct gcgaggacga ggcgccgagc
     9901 gcgctcgcga cgtccgctcc cttcccctgc tggaggagga ccaccaggat gaggaacacg
     9961 cagacgatga cgtagatcgg gacgaggacg taaatcatgc gcgagtctcc ggcccgccgc
    10021 ccggaggcga aacgggccgg tgaggatacc ccaccggccc gaaaaggcca aaatcggccc
    10081 ggaaaggccg gagcctcagg ccccggcggc gacggccagc gcggcgaagt cggccgggac
    10141 gagcgacgcc ccgcccacga gccccccgtc gatgtcttcc tttccgaaga gctccttcgc
    10201 gttcgcgggg gtcaccgacc cgccgtagag gatgcgcgtc gcgaccgcgg ccgcggggcc
    10261 gaaacgctcg gagaggcggg cacggaggaa ggcgtgggcg tccgccgcca tctcgggcgt
    10321 cgccgcgcgc cccgtgccga tcgcccagac cggctcgtag gcgaggacga agccgggcgg
    10381 cagggcgtcg ccgaacgcgt cgagggcggt catctgcgcg gccaggacgg cctccgtcct
    10441 tccgccgtcg cgctcctcga gcgtctcccc gacacagtag acgggggaga gcccggcgtc
    10501 ggcgaggcgc ttcatcttcc gcgtgaaatc cgcctcggcc tccttccggt ccctccgcct
    10561 ctcgctgtgc ccgacgagga ccatcgcgac gccgacgtcg gcgagcatcg ccaccgagac
    10621 ttctcccgtg tgggccccct tcggctcgac gtggacgtcc tgcgcggcga gcctcacccg
    10681 cgtcgggagg aggtgccggc ccacccgctc gagggcgggg aaggcggggg cgatcgcgag
    10741 ctcgacccgc tccggcagcg ccgggaggcg cgagaggaag tcgtcgcaga agacggcggc
    10801 ctcggagggg gtcttgttca tcttccagtt cgcgatgacg agcggcgtgc gcgaggggtg
    10861 catcggacct ccatggggcg ggtccggcgc gccggccccg cgggttctgc ggacgggttc
    10921 ggggtctaga ggagcgcctc gacgcccggc agcttcttgc cgctgatcag gtcgagggag
    10981 gcgccgccac cggtcgagac gtgggagatc ttcggtccga ggcccgcctc gttgatcgcc
    11041 tcgacgctct cgccgccgcc gacgacggtg aaggcgcccg actcggccat cagcttcgcg
    11101 acgccgcgcg tcccggcgtc gaacggcttc gtctcgaaga cccccatcgg gccgttccag
    11161 aagatcgtct tcgccccctt cgccatccgg gcgaacatct cgagcgtctt cgggccgacg
    11221 tcgagcgccg cctggtcggc gggtatggcg ttcatcgcga ccgccttcgc ctggccggcc
    11281 gcctcgagcg acggcgcgac gaggaagtcc gacgggagga cgaggttcac gccccgctcc
    11341 ttgcagcggt cgatcacctt ccgggcgatc gggaccccct cgggctcgag gagcgacttg
    11401 ccgatgccga ggccgagcgc gacgacgaag tggttcgcca tccccccgcc gacgaggagc
    11461 gtgtcggccc gctcgacgag ggcttcgagg gcggcgatct tcccgaggat cttggcgccg
    11521 ccgaggatcc cgacgaacgg ccggtcgatg tcgtcgacga ccttcccgag cgcgttcagc
    11581 tccttctcca tcaggaggcc gacgccgcgg gcgccgggac cgaagtgggc cgccatcccg
    11641 gccgtcgagg cgtgggcgcg gtgcgccgtg ccgaacgcgt cgttcacgta gacctctgcg
    11701 agtgaggcga gggccttcgc gaaggcgggg tcgttcccct cctcgcccgc gcggaagcgg
    11761 gtgttctcga ggaggaggac gccgccatcg gcgagggcct tcgcggcccc ctctgccgcc
    11821 tcgccgacac agtcgccggc gaacgcgacg ggcgcgccga ggagcgcctc cagcttcttc
    11881 gcgacggggg agagagagta cttcgggtcg ggcgttccct tcgccttgcc gaggtgggaa
    11941 gccaggatca gccgcgcccc cttctcgcgg gcgaggcgga tcgtcgggag ggcctcggtg
    12001 atccgcgtgt cgtccttgac gacgccctcc ttgatcggga cgttgaagtc gacgcggagg
    12061 aagacccggc gggaggagag ctcgaggtct cggatgctgc ggggaagcgt ggccatcgga
    12121 agactcctcg ggtccgtccg ctttggcgcg agccgggacg gaccggcacc tcgtggcgag
    12181 gatcgattct atagtccgcg cgtgagcgag acccctctcg ggacgcgcgt ccgcccgatc
    12241 gacaccgcgt ccggccgcct ccgcctcctc gatcagagac gcctcccgcg ggaggagacg
    12301 tggatcgact gcgattccgc cgacgcgacg gccgaggcga tccggagcct ggccgtgcgg
    12361 ggcgcgcccc tcatcggcgt cgcggcggcc tggggcctcg cggtcgaggc gcggcggctc
    12421 gcgggcgccg acgggggctt cgacgcctcc tgggaggccg ccgccgatcg cctcgggaag
    12481 gcccgcccga ccgcggtgaa cctccgcttc ggcgtcgaga ggatgcggac ggcgttccgc
    12541 gcctccgccg gacggccgcc ggccgagagg gtcgccctcc tcgaggccga ggcggcccgg
    12601 ttcgaagagg aggaccgcga ggcgtgcgcc gcgatggggc gtcacggcgc cgcctggatc
    12661 gccgcgcacc tcggcgcgga cccggccggt ccgctgcgcg tcctgaccca ctgcaacgcc
    12721 ggggcgctgg cgacgaccgg cgtcgggacg gcgctcggcg tcgtccgcgc cctcgcggcc
    12781 ggccgccccg tcgccgtctt cgccgacgag acccgtccct tctggcaggg ggcgcgcctc
    12841 accgcctggg agctcgcgaa ggacgggatc gacgtgacga tcctccccga cgtcgcggcg
    12901 gcctccgtca tcgcttcggg gaaggtctcc gtcgccgtcg tcggcgcgga ccggatcgcc
    12961 gcgaacggcg acgtcgcgaa caaggtcggg acctacggcg tcgccctcgc ctgccgggcg
    13021 cacggcgtcc cgttcgtcgt cgcggccccg cggacgacgc tcgactccgc ctgccccacg
    13081 gggcgcgaca tcccgatcga gtggcgcgac ggcgcggagg tcctcctcct cgacccggcg
    13141 ggcgagagcc cgctctccgt cgcccccgcc ggcgtccccg ccctctaccc ggccttcgac
    13201 gtcaccccgg cggccctcgt cgacgcgatc gtgaccgagg cgggcgtctc gaccgcgccg
    13261 cacgccgacg ggctcgcgag gctcctcgcc cttccgtgac gtacgtcctt ggcctcgaga
    13321 cctcctgcga cgagacctcg gtcgccctcc tccgggagga cggctcgatc gccgcgtcgg
    13381 tcgtctcctc ccagatcgac atgcacaagc gttacggcgg cgtcgtcccc gagatcgccg
    13441 cgcgggcgca cctgacgaac ctcccgccgc tcctcgacga ggcgctcgag caggccgggg
    13501 cacacctcca ggaggtcggc ctcgtcgtcg cgacggccgg cccggggctc gtcggcgccc
    13561 tcctcgtcgg cctcgcggag gggcgcgggc tcgcgctcgg tctcggcgtc ccgttcgtcc
    13621 cggggcacca catcgagggc cacgccctct cgcccttcct cgacctcgac gggggcccgg
    13681 cgcgggccgt cccgccccgc ttcgcggcgc tcgtcgtctc gggagggcac tcgcacctct
    13741 tctcgttcgc gccgaaaggg atctccgtcc tcgcgaagac gcgcgacgac gctgcgggcg
    13801 aggcctacga caaggtcgcg aagatggccg ggctcggcta tccgggcggc ccggtcgtcg
    13861 accgcctcgc ccggagcgga cgggtcgtcg agccgttcgc ggtcccgcgc ttcaagggag
    13921 gctcacacga cttctccttc tccggcctga agacggccgt ccgcgaccgc ctcgccaccc
    13981 tcggcttcgc gccgaacacg ggggtcgcgc ttcgcggccc ggcgcttccc gaggaggagg
    14041 ccccgccggc cctgcgcgac ctcctcgccg cgttccagga ggccgccgtc gcccagctcg
    14101 aagaccgggt cgaacggctc cacgagtcgg agcgcttccc gctcctgacc gtctccggcg
    14161 gcgtcgcggc gaactcgctc cttcgggagc ggctcgcggc ctgggggacg cggcgcggcg
    14221 tcgaggtcct cctcgctccc cgggctctca cctccgacaa cgcggcgatg gtcgccttcg
    14281 ccggcctcct ccggcacagg gccggggcgc cgcgcgaggg cctctcggcg ccggcccgct
    14341 cccgctggcc gctcgagggg ctgccgtggg gagcttcggc cgggccgccg aggttccgct
    14401 agaatcaaag gttcgggcgc gaggcccgac ccacaccatg cccgaccctt cccgcatccg
    14461 aaacttctcg atcgtcgccc acatcgacca cggcaagacg accctctccg accggatcct
    14521 cgagatcacg ggggccctga gcgcgcgcga gatgcgtgaa caggtcctcg acgacaaccc
    14581 gatcgagcag gagaggggga tcacgatcaa ggcccgcgcg gtctcgctca agtaccggag
    14641 cgaggacggc caggactggg tcctgaacct gatcgacacg ccgggacacg tcgacttcac
    14701 ctacgaggtc tcccggtccc tcgcggcctg cgagggggcg gtcctcgtcg tcgacgccac
    14761 ccagggcgtc gaggcgcaga ccctcgcgaa cgcctacctc gcgatccacg gcgacctgac
    14821 gatcgtcccc tgcatcaaca aggtcgacct cccgagcgcg gtccccgaga tggcccgcga
    14881 gcagatcgag gaggtcatcg ggatcccggc gcagcacgcc gcgctcacgt cggggaaggt
    14941 cggaacgggg gtccgcgacc tcctcgagca catcgtccac gacgtcccgg cgccgacggg
    15001 agacccgaac ggcccgctgc gcgccctcct cttcgactcc tggttcgacg tctaccgggg
    15061 cgtcacctgc ctcgtccgcg tgaaggacgg cgcgatccgg cccgggatga agatcaagat
    15121 gctcgggatg gacctcgtct ccgaggtcca ggaggtcggc gtcttcacgc ccaaggccca
    15181 ggtctccccc gagctgacgg tcgggatggt cgggtacgtc atcgccggca tcaaggacct
    15241 ccactccacg aagatcggcg acacgatgac cgaggccgag cgcccggcgt ccgagccgct
    15301 ccccggcttc caggacgtca agccgatggt cttcgcctcg atcttcccga ccgagtcgga
    15361 cgactacgag gacctgcgcg acgccatgga gaagctccgg ctgaacgacg cgtcgttcac
    15421 gttcgagccg gagtcgtcga cggccctcgg cttcgggttc cgctgcggct acctcggcct
    15481 cctccacctc gagatcgtcc aggagcgcct cgagcgcgag ttcgacctct cgctcatcac
    15541 gaccgcgccg tcggtgccgt accgcgtcac gacgacccgc ggcgaggtcc tcgagatctc
    15601 gaacccggtg aagctcccgg agacccagct catcgacgtc atcgaggagc cgcggattct
    15661 cgcgacgatc atcacgaagg acgagtacct cggcggcatc ctcgcgctct gcgaggagcg
    15721 ccgcggacga caggtctcgc tcgagtacgc cggcacccag cgcgccgtcc tgaagtacga
    15781 cctcccgctg aacgaggtca tcctcgactt ctacgacaag ctcaagtcgg tctcccgcgg
    15841 ctacgcctcg ttcgactacc agctgacggg gtacgccgag gccgacctcg tcaagctcga
    15901 cgtcctcatc aactccgagc cggtcgacgc gctcgccttc atcgtccacc gcgacaaggc
    15961 gcaggccaag gggcgcgccc tcgtcgagaa gatgaaggag atgatcccgc agcagatgtt
    16021 cgaggtgatc atccaggcgg cgatcggggc gaagatcctg gcccgctcga cggtgaaggc
    16081 gctccgcaag aacgtcctcg cgaagtgcta cggcggcgac atcacgcgga agaggaagct
    16141 cctcgagaag cagaaggaag gcaagaagcg gatgaagaag gtgggccggg tcgagctccc
    16201 gcaagaagcc ttcctggcgg tcctgaaggt cgactgaggg gccgggccgt ggcctcctcc
    16261 gccggggacg gcccggcgcc ccgcgtcccg cgccaccgca ccaccctgcg cgagtacgtc
    16321 gaggcggtcc tcgtggccgt cgtcttcgcc ctcttcgcga aggcgttcct cgtcgagacc
    16381 tatcagatcc cgagcggatc gatggaggag agcctcctcg tgggcgacca cgtcgtcgtc
    16441 gacaagctcg gctgggcgcc gcgcggcctc ccctgggggc ccctcctccc gtcgcgcggg
    16501 ctccggcggg gcgacgtcgt cgtcttccgc ggaccggagg agcccgggcg ggacttcatc
    16561 aagcgcgccg tcgccctgcc gggcgagacc ctcgagatcc gcggaaagcg gctcttcgtc
    16621 gagggcgtcg cccaggcgga gccctgggtc gtgcaccggg accccgccgt cctctccggg
    16681 gagggcctcc cggcatccgt ccgggggcga gacgagctgg cgccccggac gatcccggcg
    16741 gagacgttct tcgccctcgg cgacaaccgc gacgagtccc gggactc
//
LOCUS       DAOONL010000075        16560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3675_length_16560_cov_15.098394, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000075 DAOONL010000000
VERSION     DAOONL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_16560_cov_15.098394"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="pilM"
                     /locus_tag="PLP50_11480"
     CDS             complement(<1..335)
                     /gene="pilM"
                     /locus_tag="PLP50_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPA52212.1"
                     /translation="MLFRKAKNLVGLDIGSSAIKLVELRAAKEGQFQLVKAGLENLSP
                     EAIVDGAIMDSSLVVETISRLLASTGIKNPNVAVSVSGHSVIVKKIQLPTMPEEELSE
                     SIRWEAEQYV"
     gene            590..784
                     /gene="rpmB"
                     /locus_tag="PLP50_11485"
     CDS             590..784
                     /gene="rpmB"
                     /locus_tag="PLP50_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPA52213.1"
                     /translation="MPQMCEVCGKAPVAGRRVSHAHNVTNRQFKPNLRRVRAAVPGGS
                     KRVTVCTRCLRSDKITKVVR"
     gene            complement(838..3018)
                     /locus_tag="PLP50_11490"
     CDS             complement(838..3018)
                     /locus_tag="PLP50_11490"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPA52214.1"
                     /translation="MLRFEDILDAVESYLPGADEDLLRRAYIFSAQAHRHQLRSSGEP
                     YLVHPIGVAMLLAELKLDEVSVATGLLHDVLEDTKTTKEELATLFGSEVAEVVDGVTK
                     IGRYAYTSKEAQQAETFRKMLLAMTGDLRVILVKLADRLHNMRTLHHLREEKRRVIAA
                     ETMEIYAPLANRLGMGRIKGELEDLSFRYLHPDEFIALSAAVDERMKASGAAIESLRV
                     DIGARLEEAGVPAEVTGRVKRLWSIRQKLIRQSIPLDQLYDVLAFRVLVDSVRDCYSA
                     LGIVHQAWRPVPGRIKDYIAMPKQNFYQSLHTTVIPEGEPPFEIQIRTREMDLVAEQG
                     IAAHWKYKEGRGSRKVDEEGIAAIRQILETTRDIANPREFLSSLKMDLYADEVYTFTP
                     KGAVYSFPRGATSVDFAYRIHTDVGHRCVGARVNGRLVPLRTPLVNGDIVEILTAPSG
                     APSRDWLAFVVTSRARNKIRAFLQTKEKQKSIEIGRRFLEKELKKVKRSFGRLLESKA
                     FEPLLPELGVPRVDDLLAEIGYGKVLPRAVVQKLFPQEEPSAEKGPSRTDALKKVVRK
                     ILPIGGTGILVRGENDLLASLAKCCRPVPGEEIVGYVTRGRGVSVHAAACPNVRNLLF
                     DPSREIEVEWHVGKDASFTVDFVVEVEDRPGILARITQVISDAESNIESIEARTSPGG
                     TALIEGSVTTPGRRHLDKLFLAIRSVPGVTGVRRKFNVSRLGSR"
     gene            3084..3773
                     /locus_tag="PLP50_11495"
     CDS             3084..3773
                     /locus_tag="PLP50_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HPA52215.1"
                     /translation="MPAPAPEPETAPTDPSADPAAGGLHPLLRALSAPRRWLRATYDW
                     TMRWADKPHALAALFLIAVVESSVFPIPPDVLLIAIVAASPRLWLRSAALCTLGSVIG
                     AAGGYLIGTAFMATLGQRIVEFYNAQHHWAKVVELYNGEWGIWFLAAAAFTPIPYKVA
                     TIAAGATGMAFVPFLLVSLVGRAARFFLVAALLRVFGPAIRRTLEKNFDLAALAFLVL
                     LVGGFLALKYF"
     gene            complement(3828..4847)
                     /locus_tag="PLP50_11500"
     CDS             complement(3828..4847)
                     /locus_tag="PLP50_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPA52216.1"
                     /translation="MTSVSLFPFAETWWVYGLFVVFVVGMLALDLGVFHRKAHVVGFR
                     EAAAWSAVWVVVAMGVGTVLYVWASARFAADPRLLAVPGFVPDAAARQVFLEFLTGYV
                     IEKALSVDNIFVFVVLFQFFGVPAAYQHRVLYWGILGALVFRAIFIALGALLLQFHWV
                     MYVFGAFLVITGVKLIFGPDKPVDPADNAVLKALKRWMPLTHELHGPHLFAREGGRLM
                     GTPLLLALVFIEMSDVVFAIDSVPAIFAITREPLLVFTSNICAILGLRALFFLLAGAV
                     GKFHLLKYGLGLVLVFVGLKMAWLNDAFGGHFPIGLSLGIIGALLVASIVLSLAFPPR
                     EAGAE"
     assembly_gap    4896..4995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4996..6145)
                     /gene="secF"
                     /locus_tag="PLP50_11505"
     CDS             complement(<4996..6145)
                     /gene="secF"
                     /locus_tag="PLP50_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPA52217.1"
                     /translation="MRLLRNPNFDFLKLQKPFVIGSIVAALISAGAIATKGLNWGVDF
                     TGGAQIVYAFQQTPDEDQVRKIVESAGVPVTSVQRYDKAEKNQILLRVPMEQKEGRDV
                     SGEVTPALVKALSPAGRGDGVFDLNLNGADPLSAKLQQDDPEKMAGRADANPAVEYAR
                     IAQAIIAHRSQTGLFASLDAAAATPGLSPAVASWLKEKTVAGPVTLIGAESVGPAVGK
                     DLRRKGTWAVVLSWGAILVYVWFRFRSVSYGTGVVLALIHDAFIALGLCAIFGVEISL
                     TVVAAFLTLIGYSANDTVVIYDRIRENLEKPKKEPFALLVNRSINETLSRTFLTQFLT
                     FLTVAALFFFGGEVIRGFSFVLFVGAFIGAYSTIFIAAPWVVNWETWKA"
     gene            complement(6153..7784)
                     /gene="secD"
                     /locus_tag="PLP50_11510"
     CDS             complement(6153..7784)
                     /gene="secD"
                     /locus_tag="PLP50_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPA52218.1"
                     /translation="MKRPTGWRLALTLVVLIGSIAAYVYRGREALKKNPTAPPNPTVG
                     DVLKNGLKLGLDLRGGIHLVMQVKAEDALRAEANDAAEHLKTEAGKRGVTIGTVNPAT
                     HEGFTITMGEDVSPAAVSDLVKSALSPTDWASDQSGREMRVTFREPARIEIEGQALRQ
                     AVETIRNRVDALGVAEPAIQPQGNDRIVIQLPGVDDPARVKDIIGTTGLLELKIVDLK
                     GGPYSSPEAAAAAYGGTIPANLELVEGNAEDEEARTRTTVYYVLERASAVTGRDLKNA
                     RVGQDRFNQPAVEFFLNAAGAEKFGRVTGDNVGRQLAIVLDKRVQSAPNIKTQITDNG
                     IIEGNFTPERADSLALVLRSGALPAQLVILEERTVGPSLGLDSIKQGILAALVGIFLV
                     FIAVVVYYNLAGLNAVLALTMNAIILLGAMAWINATLTLPGIAGFILTIGMAVDANVL
                     IFERIREELDGGKAAKAAIDLGFKKALSAIVDSNLTTIAAAAFLFQFGTGPVKGFAVT
                     LVIGLCASMFTAVFVSRLLFDLLYGSKEKVEAISI"
     gene            complement(7895..10807)
                     /locus_tag="PLP50_11515"
     CDS             complement(7895..10807)
                     /locus_tag="PLP50_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52219.1"
                     /translation="MERVVVEGFGPLEGIDLSWGDGELLLVLEPNETGKSTLCEAITA
                     ALYGLPKGRSAGGRSREARRPRSGAPARLRLDVAAAAGRFSIERDFDEGTLRVVDRDR
                     GEDRTAEFLRAGGRDAVGEKLTGLTEALFRTTAYVGQNVLDGDALDSSLTVELARIAD
                     SGGGEASVVRALRLLETARARMPEATTGAQVSVETEIVRASRRVEEKRAEVARLAKAR
                     EAAAEAAGRLAARTAERDAARRAAALAEVAVVESERRSLSAELERRRTEKRRRVELEA
                     EARSLEREAVLFAPETLAAVDALRSSRGSRPEALVAARAALAAERAAAQAEDRERRKR
                     AGGLADLDPEARALLRRHLEAAREAEVESEAAEKALEAEWQELRREGLAEDLRRLDGL
                     DAADRDFVAGAEESRQGLELEGVQLDRRFADAGARASIVAGERRVLVRRARVLVVAAG
                     LVAPLAVGTLFRRGRLDDPLVLMLFAFGLVLALYGGIAWARGNRYGREEEARFRQEEA
                     SARAEAQETRRRLSEVRLRLEQIARGAGFKDSLGLLKAQRKARAADEERQRLVEKRVR
                     LFAAGERLAAATKGLEPYRAALGLATGLPSDDESRRVFQMLTDLEKADSALAGRRDAA
                     EREEERLATEERELRRLEEELAGLLSRLEVPAGLSLPESLLFVEAGRARAARRREIQE
                     VELPALAVASPPDGDETVEERVRLLAAEVEMRLDVLGAALPDLSVPAEPEAARRAAEV
                     LRERAARLEEERQGAERELAQRALEGGERGREAEEQLAALDVARERAVLYRDALDAAR
                     EVLASAATTTYGDFRAGLARASREILARCRLPYEALEFGEDLSVSVVGRGGRPVTRAE
                     LGASVSTGAREQLHLVARLAALRHLGTGPRGLPLLLDDPLVGADDDRFDAVLEFLLDD
                     VLTERPVLLVSCHRSRHERWLETLSTERAARVRRVPLASRRRAGPDRELTPAPGGE"
     gene            complement(10810..12012)
                     /locus_tag="PLP50_11520"
     CDS             complement(10810..12012)
                     /locus_tag="PLP50_11520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPA52220.1"
                     /translation="MAPLVLLQSSDWHVGSPLTGRGLGLGEELRALRRREVDEAPQRL
                     VRAAREAGPDAILLPGDLWDAGSVPPTLFRRLVEALEALAPVPVFVAPGNHDFAGPGG
                     FYDDAFLDALGLPDWPGNVRVFRSSAWETAAVPGRDDATVTGRAFLSPLVDTGRPLAP
                     PPARPAAELAILMLHGSLESYRGPDAPSGSKKTAPFSREELLGAGFDWTALGHHHRME
                     VVTREDGSPAGAYSGSPTGRGLDETGPRHFVKVTLEAGAPARVETLPADCRKVLDLEL
                     DAAGRDGEGLRDAALALLLEAGATPGDLVRLTVVGRSAPATRTTQTLGDLKGLVAHLA
                     LRDRTLGDGEARPDRGTAEGRFVLDLESRLAATEDPRARRVLELAAVLGREALLGRVP
                     APPAEEDL"
     gene            complement(12041..12382)
                     /gene="yajC"
                     /locus_tag="PLP50_11525"
     CDS             complement(12041..12382)
                     /gene="yajC"
                     /locus_tag="PLP50_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPA52221.1"
                     /translation="MNPIALLQAAPAAPSMKDGLLQMVPFLLIFVVFYFVLLAPMRKQ
                     QKKTKEMLAQLKKGDRVMTSGGIYGTVAQVEDQIVWVKLADTLKVKMAKSAITGVVAD
                     TEGGKDQGSAA"
     gene            complement(12379..13512)
                     /gene="tgt"
                     /locus_tag="PLP50_11530"
     CDS             complement(12379..13512)
                     /gene="tgt"
                     /locus_tag="PLP50_11530"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPA52222.1"
                     /translation="MLSFELLAVDGAARRGRVTLDHGTVETPAFMPVGTAGTVKAVRW
                     SDVSAAGAEIVLANTYHLMLRPGGELIREMGGLHAFVGWGRPILTDSGGFQVMSLAAN
                     RKLTEEGVVFRSHLDGSAHLLTPERAAELQLRDFGVDVAMALDECTPYPATRDEAARS
                     MALTHRWARRGRAAFLAAREGSGRGAQFGIVQGGMYADLRRESAAAVAAEGFEGIACG
                     GVSVGEPKEEMRAIVEATGPLLPPGRPRYLMGVGHPGDLLHAVASGFDLFDCVLPTRA
                     ARHGLAYTSEGTVVLKQARFRTDRAPLDPACACPTCATASRAYLRHLFMLGEPTAATL
                     VTVHNLHAILDLMRGIREALSARRYAAFAAEAAGRLDFGKDKR"
     gene            13660..13992
                     /locus_tag="PLP50_11535"
     CDS             13660..13992
                     /locus_tag="PLP50_11535"
                     /inference="COORDINATES: protein motif:HMM:NF021083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HPA52223.1"
                     /translation="MPDLGQKHECFRCGTKFYDLKRTPVFCPKCGADQKDAPPRDTSP
                     AALIAARRAKRDEFADPDLFERNRDEDDLGAEPEDPNAKAALDDEELPLGEGPSLEAG
                     DSESEDDY"
     gene            14012..14488
                     /gene="folK"
                     /locus_tag="PLP50_11540"
     CDS             14012..14488
                     /gene="folK"
                     /locus_tag="PLP50_11540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPA52224.1"
                     /translation="MGLGGNVGSPRLAFEAALAGLSSSVHGLVLSPVYRTAPVGGPPQ
                     PDFLNAVAVGRTRLEPVALLATLLRLEAEAGRRRSPLRDGPRPLDLDLLLYGDLVLEL
                     PGLVLPHPRMASRRFVLAPLADLRPGLVVPGTGATVSDLLAAAPPARVQRVAYENA"
     gene            complement(14492..15412)
                     /locus_tag="PLP50_11545"
     CDS             complement(14492..15412)
                     /locus_tag="PLP50_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPA52225.1"
                     /translation="MKERIRGAMARIVATAESRPQLSAVEAAPRPQKKEIIEILRLFQ
                     EVVYPGYFGDTVLYRDNLRDHLGDVLFRIHLRLSAEVEKALEASEGRPAARRHAREVV
                     TELFERMPEVADRVAEDVQAAYDGDPAAQGFDELILAYPGVKAIFTYRIAHELHRMGV
                     RLIPRVMTEFAHNETGVDIHPGATIGRSFFIDHGTGVVIGETTTIGDRVKIYQGVTLG
                     ALSFPKNEHGELIRGVKRHPTVEDDVVIYAGATILGGSTVIGRGSVIGGSVWLTTSVP
                     AFTRVTLAGEQLRFEPRMSPARGRGPDAPA"
     gene            complement(15409..16311)
                     /locus_tag="PLP50_11550"
     CDS             complement(15409..16311)
                     /locus_tag="PLP50_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52226.1"
                     /translation="MQRTTRGVPASVILVSFLLGPASVLAADPSATVAAADAAWARRA
                     AGATGGTAADAAARDVVAAARAAVAAEPESVGARWRLMRALYFLGEHATPGEAAKKAV
                     FDEGKRVGEETLALLRRTAGASAGRDLTSASPVELAPYLAQTPDAVPAFLWAGVNWGK
                     WALVFGKTAAARQGAAAKIRDYAAAVVALDPTWDEGGGYRVLGRLHHQTPSIPFVTGW
                     ASRAEALASLRRAVEKGPRNFVNRLYLAEAIWDYEKGKKDEARGMLEALVREAPTPGL
                     EVEAARAQEDARALLAAWAGKGRR"
BASE COUNT         2336 a         5956 c         5847 g         2321 t
ORIGIN      
        1 acgtactgct ccgcctccca ccggatcgac tcggagagct cctcctccgg catcgtcggc
       61 agctggatct tcttgacgat gacggagtgg cccgagacgg agacggcgac gttcgggttc
      121 ttgataccgg tcgacgcgag aaggcgggag atcgtctcga cgacgagcga ggagtccatg
      181 atcgcgccgt ccacgatcgc ttccggcgag aggttttcca ggccggcctt gaccagctgg
      241 aactgtccct ccttcgctgc ccggagctcg accagcttga tggcggacga gcctatgtcg
      301 agaccgacga ggttcttggc cttgcggaac aacacggtgc ggtctccctc cctctacgtt
      361 ccggaaccga cgcgacaccc tgacacgagc tgggtaaagc ctttgtggcg gctatgttgc
      421 gcgggtcccc cgagactgtc aaggacgaga aaagcccgaa atgcctctgg acacggagac
      481 ggaggggcgc tataagtcgc agttccggcg gcgaccccgg tccgctccga cgaggacggg
      541 accggagcgt cgccgggtat cgcgttcagg attcagaggg agccacccca tgccccagat
      601 gtgcgaagtc tgcggaaagg ccccggtcgc cggtcgccgg gtcagccacg cccacaacgt
      661 cacgaacagg cagttcaagc cgaacctcag gagggtccgg gccgcggtcc ccggaggctc
      721 caagcgggtc acggtctgca cgcgctgcct tcggtccgac aagatcacca aggtcgtccg
      781 ctgacgcctc cgccggccgc ggggaacccc tcctcgcgac cggcgtgacc gagcccttca
      841 gcgcgacccg agccgggaga cgttgaactt ccgtctcact ccggtcaccc cgggcacgga
      901 gcggatcgcg aggaagagct tgtcgaggtg gcggcgcccc ggagtcgtga cgctcccctc
      961 gatcagcgcc gttccgccgg ggctcgtccg ggcctcgatc gactcgatgt tcgactccgc
     1021 gtccgagatg acctgggtga tccgcgccag gattcctggg cggtcctcga cctcgaccac
     1081 gaagtcgacc gtgaacgacg cgtccttccc cacgtgccac tcgacctcga tctcgcggct
     1141 cgggtcgaag aggaggttcc tcacgttcgg gcaggccgcg gcgtggaccg agaccccgcg
     1201 cccgcgcgtc acgtagccga cgatctcctc tccgggcacc ggcctgcagc acttcgcgag
     1261 gctcgcgaga aggtcgttct cgcccctcac gaggatgccc gttccgccga tcgggaggat
     1321 cttccggacg accttcttca gggcgtcggt ccgcgacggc cccttctccg cggacggctc
     1381 ctcctggggg aagagcttct ggaccaccgc gcgcgggagg accttgccgt agccgatctc
     1441 cgcgaggagg tcgtccacgc gcgggacgcc gagctcggga agcagcggct cgaaggcctt
     1501 cgactcgagg aggcggccga aggagcgctt caccttcttc agctccttct cgaggaagcg
     1561 ccgcccgatc tcgatcgact tctgcttctc cttcgtctgg aggaaggcgc ggatcttgtt
     1621 ccgcgcgcgg gaggtgacga cgaaggcgag ccagtcgcgc gacggggcgc ccgagggcgc
     1681 cgtgaggatc tcgacgatgt cgccgttgac gagcggcgtc cggagcggga cgagccggcc
     1741 gttcacgcgc gcgccgacac agcggtgccc gacgtcggtg tgaatccggt aggcgaagtc
     1801 gaccgacgtc gcgccccgcg ggaacgagta gacggcgccc ttcggcgtga acgtgtagac
     1861 ctcgtcggca tagaggtcca tcttcagcga ggagaggaac tcccgcgggt tcgcgatgtc
     1921 gcgcgtcgtc tcgaggatct ggcggatcgc cgcgatcccc tcctcgtcga ccttccggct
     1981 cccgcgcccc tccttgtatt tccagtgcgc cgcgatcccc tgctccgcga cgaggtccat
     2041 ctcgcgcgtc cggatctgga tctcgaacgg cggctcgcct tccgggatca ccgtcgtgtg
     2101 gagcgactgg tagaagttct gcttcggcat cgcgatgtag tccttgatcc ggcccgggac
     2161 gggccgccag gcctggtgga cgatgccgag ggcgctgtag cagtcgcgga ccgagtcgac
     2221 gaggacgcgg aacgcgagga cgtcgtagag ctggtcgagc gggatcgact gacggatcag
     2281 cttctggcgg atggaccaga ggcgcttcac gcggccggtc acttccgcgg gaacgcccgc
     2341 ctcctcgagg cgggcgccga tgtcgacgcg gaggctctcg atcgcggcgc ccgacgcctt
     2401 catccgctcg tcgaccgccg ccgagagggc gatgaactcg tcggggtgga ggtaccggaa
     2461 cgagaggtcc tcgagctcgc ccttgatccg gcccatcccg agccggttcg cgagcggcgc
     2521 gtagatctcc atcgtctcgg cggcgatcac gcgccgcttc tcctcccgga ggtggtggag
     2581 cgtccgcatg ttgtggagcc ggtcggcgag cttgacgagg atgacgcgga ggtcccccgt
     2641 catcgcgagg agcatcttcc ggaacgtctc cgcctgctgc gcctccttcg aggtgtaggc
     2701 gtagcggccg atcttcgtga cgccgtcgac gacctccgcc acctcgctgc cgaagagcgt
     2761 cgccagctcc tccttcgtcg tcttcgtgtc ctcgaggacg tcgtggagga ggccggtcgc
     2821 cacggagacc tcgtcgagct tcagctcggc gaggagcatc gccacgccga tcgggtggac
     2881 gaggtaaggc tcgcccgagg agcgaagctg gtggcggtgc gcctgggccg agaagatgta
     2941 cgccctccgg aggaggtcct cgtcggcccc cggaaggtag ctctcgaccg cgtcgaggat
     3001 gtcctcgaac ctgagcacgt ccggattatg gcgtccccgt ctccgcgctg cgcgccgggc
     3061 gaggttcggg taacctccgc cggatgcccg caccggcccc cgagcccgag accgctccga
     3121 ccgacccgtc cgccgacccg gccgccggcg gcctccaccc gctcctgcgg gcactctccg
     3181 cgccccggcg ttggctccgg gcgacctacg actggacgat gcgctgggcc gacaagccgc
     3241 acgcgctcgc ggccctcttc ctgatcgccg tcgtcgagtc gtcggtcttc ccgatccctc
     3301 ccgacgtcct cctcatcgcg atcgtcgccg cctcgccccg cctctggctc cgctccgcgg
     3361 cgctctgcac gctcggatcg gtgatcggcg ccgcgggcgg ctacctcatc gggaccgcgt
     3421 tcatggcgac gcttggccaa cggatcgtcg agttctacaa cgcgcagcac cactgggcga
     3481 aggtcgtgga gctctataac ggcgaatggg ggatctggtt cctcgccgcc gcggcattca
     3541 cgccgatccc ctacaaggtc gccacgatcg cggccggagc gaccgggatg gcgttcgtcc
     3601 cgttcctcct cgtcagcctc gtcggacggg cggcccgctt cttcctcgtc gccgccctcc
     3661 tccgggtctt cggcccggcg atccgccgga cgctggagaa gaacttcgac ctcgcggccc
     3721 tcgcgttcct cgtcctcctc gtcggcggct tcctcgcgct gaagtacttc tgaggcgccg
     3781 ccggccgcga gccctccggc gggctcgggc ggctccggag gaggcgccta ctccgcgccc
     3841 gcctcgcgag gcgggaaggc gagcgagagg acgatcgagg cgacgagcaa ggcgccgatg
     3901 atcccgagcg acaggccgat cgggaagtgc ccgccgaagg cgtcgttcag ccacgccatc
     3961 ttcagcccga cgaagacgag gacgaggccg aggccgtact tcagcaggtg gaacttgccg
     4021 accgcgcccg cgaggaggaa gaagagcgcg cggagcccga ggatcgcgca gatgttcgac
     4081 gtgaagacga ggagcggctc gcgcgtgatc gcgaagatcg ccgggaccga gtcgatcgcg
     4141 aagacgacgt cggacatctc gatgaagacg agggcgagga gaagcggcgt ccccatcagg
     4201 cgcccgccct cgcgggcgaa gaggtgcggc ccgtggagct cgtgcgtgag cggcatccag
     4261 cgcttcagcg ccttgaggac cgcgttgtcc gccgggtcga ccggcttgtc cggcccgaag
     4321 atcagcttga cccccgtgat cacgaggaac gccccgaaga cgtacatgac ccagtggaac
     4381 tggaggagga gcgcgccgag cgcgatgaag atcgcccgga agacgagggc cccgagaatg
     4441 ccccagtaga gcacgcggtg ctggtaggcg gccgggacgc cgaagaactg gaagaggacg
     4501 acgaagacga agatgttgtc tacggaaagc gccttctcga tgacgtagcc cgtcaggaac
     4561 tccaggaaga cctgtcgggc cgccgcgtcg gggacgaagc ccggtacggc caggaggcgc
     4621 gggtcggccg cgaagcgagc cgaggcccag acgtagagga ccgtgccgac gcccatcgcc
     4681 accacgaccc agaccgcgct ccaggcggcg gcctcgcgga acccgacgac gtgcgccttc
     4741 ctgtggaaaa cgccgaggtc gagggcgagc atcccgacga cgaagacgac gaagaggccg
     4801 tagacccacc aggtctcggc gaaggggaag agggagacgc tcgtcatcgg ggtcctctcg
     4861 ggcggtccgt gggtccgggc gccggggcgc cctcannnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnntcgcc ttccacgtct cccagttcac gacccagggg gccgcgatga
     5041 agatcgtgga gtaggcgccg atgaacgcgc cgacgaagag gacgaacgag aagccgcgga
     5101 tcacctcgcc gccgaagaag aagagggcgg cgacggtgag gaacgtcagg aactgggtca
     5161 ggaacgtccg cgagagcgtc tcgttgatcg agcggttcac gaggagcgcg aacggctcct
     5221 tcttcggctt ctcgaggttc tcgcggatcc ggtcgtagat gacgaccgtg tcgttcgccg
     5281 agtagccgat gagggtcagg aaggcggcga cgaccgtgag cgagatctcg acgccgaaga
     5341 tggcgcagag gccgagcgcg atgaacgcgt cgtggatcag ggcgaggacg acgccggtgc
     5401 cgtacgagac cgaccggaag cggaaccaga cgtagacgag gatcgcaccc caggagagga
     5461 cgacggccca ggtccccttc cggcggaggt ccttcccgac cgcggggccg accgactcgg
     5521 ctccaatgag cgtcaccggg ccggcgaccg tcttctcctt cagccaggag gcgacggcgg
     5581 gcgagaggcc gggcgtcgcc gcggcggcgt cgaggctcgc gaagagcccc gtctgcgagc
     5641 ggtgcgcgat gatcgcctgg gcgatgcggg cgtactcgac ggccgggttc gcgtcggccc
     5701 ggccggccat cttctcgggg tcgtcctgct ggagcttcgc ggagagcggg tcggcgccgt
     5761 tcaggttcag gtcgaagacc ccgtcgcccc gccccgcggg ggaaagcgcc ttgacgagag
     5821 ccggcgtcac ctcgccggag acgtcccgcc cctccttctg ctccatcggg acgcggagga
     5881 ggatctggtt cttctcggcc ttgtcgtagc gctggaccga cgtgacgggg acgcccgcgc
     5941 tctccacgat cttgcggacc tggtcctcgt cgggggtctg ctggaacgcg tagacgatct
     6001 gggcgccgcc ggtgaagtcg acgccccagt tcagcccctt cgtggcgatc gcgccggccg
     6061 agatcagcgc ggcgacgatc gatccgatga cgaagggctt ctggagcttg aggaagtcga
     6121 agttggggtt tcggagcagc ctcacgggtc cctcagatcg agatggcctc gaccttctcc
     6181 ttcgacccgt agaggaggtc gaagaggagg cgcgagacga agacggcggt gaacatggag
     6241 gcgcagaggc cgatgacgag cgtcacggcg aagcccttga cggggccggt gccgaactgg
     6301 aagagaaacg cggccgccgc gatggtcgtc aggttggagt cgacgatggc gctgagcgcc
     6361 ttcttgaacc cgaggtcgat cgcggccttc gcggccttcc cgccgtcgag ctcctcgcgg
     6421 atccgctcga agatgaggac gttcgcgtcg accgccatgc cgatcgtcag gatgaagccc
     6481 gcgatgcccg ggagcgtcag cgtggcgttg atccaggcca tcgcgccgag caggatgatc
     6541 gcgttcatcg tcagggcgag gacggcgttg agacccgcga ggttgtagta gacgacgacg
     6601 gcgatgaaga cgaggaagat cccgacgagc gcggcgagga tcccctgctt gatcgagtcg
     6661 aggccgagcg acggcccgac cgtccgctcc tcgaggatga cgagctgcgc cggaagggcg
     6721 ccggagcgga ggacgagcgc gagggagtcg gcccgctcgg gggtgaagtt cccctcgatg
     6781 atcccgttgt cggtgatctg ggtcttgatg ttcggcgccg actggacccg cttgtcgagg
     6841 acgatcgcga gctggcggcc gacgttgtcg ccggtgaccc ggccgaactt ctcggccccc
     6901 gccgcgttca ggaagaactc gaccgccggc tggttgaagc ggtcctgccc gacgcgggcg
     6961 ttcttcaggt cgcgtccggt gacggcggag gcccgctcga ggacgtagta gaccgtcgtc
     7021 cgggtccggg cctcctcgtc ctcggcgttc ccctcgacga gctcgaggtt cgccgggatc
     7081 gtcccgccgt aggcggccgc ggccgcctcg ggcgaggagt aggggccgcc cttcaggtcg
     7141 acgatcttga gctcgaggag gccggtcgtc ccgatgatgt ccttcacccg ggccgggtcg
     7201 tcgacgcccg ggagctggat gacgatccgg tcgttcccct ggggctggat cgccggctcg
     7261 gcgacgccga gcgcgtcgac gcggttccgg atcgtctcga cggcctggcg gagcgcctgc
     7321 ccttcgatct cgatccgggc cggctcgcgg aacgtcacgc gcatctcgcg gccgctctgg
     7381 tccgaggccc agtcggtcgg cgagagggcg ctcttgacga ggtcggagac ggcggccggg
     7441 ctcacgtcct cccccatcgt gatcgtgaac ccttcgtgcg tggccgggtt caccgtcccg
     7501 atcgtgacgc cgcgcttgcc ggcctcggtc ttcaggtgct cggcggcgtc gttcgcctcc
     7561 gcgcggaggg cgtcctccgc cttcacctgc atgacgaggt ggatgccgcc ccgaaggtcc
     7621 agcccgagct tcaggccgtt cttcaggacg tcaccgaccg tggggttcgg aggtgccgtg
     7681 gggttcttct tcagggcctc gcggcccctg tagacgtagg ccgcgatgga tccgataagg
     7741 acgaccagcg tcagggcgag acgccagcct gtgggccttt tcacgaaacg caacctccga
     7801 aggcggaccg gcccggacga ggaagcgctc gcctcctgaa gacctgggcc cccgactgga
     7861 atcggcccgg ctcgcccggg ccaaggcggg gagtctattc gccgcccggc gccggcgtca
     7921 attcgcgatc cgggcccgcc cggcgccggc tcgcgagcgg caccctccgg acgcgcgccg
     7981 cccgctccgt cgagagcgtc tcgagccagc gctcgtgccg cgatcggtgg caggagacga
     8041 ggaggaccgg cctctcggtc aggacgtcgt cgaggaggaa ctcgagcacg gcgtcgaacc
     8101 ggtcgtcgtc cgctccgacg agggggtcgt cgaggagaag cggcagcccg cgcggtccgg
     8161 tgccgaggtg gcgaagtgcg gccaggcgag ccacgaggtg gagctgctcg cgcgcgccgg
     8221 tggagaccga cgcgcccagc tcggcccgcg tgacggggcg cccgccgcgg ccgacgaccg
     8281 agacggagag gtcttccccg aactcgagcg cctcgtacgg cagccggcaa cgggccagga
     8341 tctcccggga cgcccgggcg agacccgcgc ggaaatcccc gtaggtcgtc gtcgcggcgg
     8401 acgccaggac ctcccgggcg gcgtcgaggg cgtcccggta gagcaccgcc cgctcccggg
     8461 cgacgtcgag cgccgcgagc tgctcctcgg cctcccggcc ccgctcgcct ccctccagcg
     8521 ccctctgggc gagctcccgc tcggccccct gccgttcctc ctccaggcgg gcggcccgct
     8581 cgcgcagcac ctcggccgcg cgacgtgcgg cttccggctc ggccgggacc gagaggtccg
     8641 ggagcgccgc gccgaggacg tccagtcgca tctcgacctc ggcggcgagg agccggacgc
     8701 gctcctcgac ggtttcgtcc ccgtcgggcg gcgacgccac ggcgagcgcg ggcagctcga
     8761 cctcctggat ctcccgccgc cgggcggccc gggcgcggcc cgcctcgacg aaaaggagcg
     8821 actccggaag cgagagtccc gcagggacct cgagccggga caggagaccg gcgagctctt
     8881 cctcgagccg gcggagctcg cgctcctccg tcgcgaggcg ctcttcctcg cgctccgcgg
     8941 cgtcccgccg ccccgcgagc gccgagtccg ccttctcgag gtcggtgagc atctggaaga
     9001 cacgcctcga ctcgtcgtcc gagggcaggc ccgtcgcgag accgagcgcc gcgcggtagg
     9061 gctcgagccc tttcgtggcc gccgcgaggc gctctccggc cgcgaacagg cgcacccgct
     9121 tctcgacgag ccgctgcctc tcctcgtccg ccgcgcgcgc ctttcgctgg gccttcagga
     9181 gcccgagcga gtccttgaac cccgcgccgc gcgcgatctg ctcgaggcgc aagcgcacct
     9241 cggacaggcg ccgccgcgtc tcctgggcct ccgcgcgggc ggacgcctcc tcctgccgga
     9301 agcgcgcttc ttcctcccgg ccgtagcggt ttccgcgggc ccaggcgatg ccgccgtaca
     9361 gcgcgaggac gaggccgaac gcgaagagca tcaggacgag cgggtcgtcc agccggcccc
     9421 gccggaagag cgtcccgacg gcgaggggcg ccacgaggcc ggccgcgacg acgaggaccc
     9481 gggccctccg gacgaggacg cggcgctcgc ccgcgacgat cgaggcgcgc gcgccggcgt
     9541 cggcgaaccg gcggtccagc tgcacccctt cgagctcgag cccctggcgg ctctcctcag
     9601 ccccggcgac gaagtccctg tcggccgcgt cgaggccgtc gagccggcgg aggtcttcgg
     9661 cgagcccttc gcggcgaagc tcctgccatt cggcctcgag ggccttctcg gccgcctcgg
     9721 actcgacctc cgcttcccgg gccgcttcga gatgccgccg gaggagggcc cgggcttccg
     9781 ggtcgagatc ggcgaggccg cccgctctct tccgccgctc ccggtcctcg gcctgggcgg
     9841 cggcgcgctc cgcggcgagc gccgcgcggg ccgcgacgag cgcctcgggc cgggagccac
     9901 gagaggagcg gagggcgtcg accgccgcca gcgtctcggg cgcgaacagg acggcctccc
     9961 gctcgagcga ccgcgcctcg gcctccagct cgacccggcg ccgcttctcg gtccgccttc
    10021 gctccagctc ggccgaaagg ctccggcgct cgctctcgac gacggccacc tccgcgagcg
    10081 cggccgctct ccgggcggcg tcccgctccg cggtccgcgc ggcgagcctc ccggccgcct
    10141 ccgcggccgc ctcccgcgcc ttcgcgaggc gggccacctc ggcccgcttc tcctccaccc
    10201 gccggctcgc ccgcacgatc tcggtctcga ccgacacctg ggcacccgtg gtcgcctcgg
    10261 gcatccgggc gcgggccgtc tcgaggagcc ggagggcacg gacgacgctc gcctcgccgc
    10321 cgccagagtc ggcgatccgc gcgagctcga cggtgaggga ggagtcgagg gcgtcgccgt
    10381 cgaggacgtt ctgaccgaca taggccgtcg tccggaagag cgcctccgtc agcccggtga
    10441 gcttctctcc gacggcatcg cgcccgcccg cgcggaggaa ctccgcggtc cggtcctccc
    10501 cgcgatcgcg gtccacgacg cgcagcgtcc cctcgtcgaa gtccctctcg atcgagaagc
    10561 gcccggccgc ggccgccacg tcgaggcgga gccgcgcggg cgcgccgctt ctcggccggc
    10621 gggcctcgcg cgagcggcct cccgccgacc ggcccttcgg cagcccgtag agcgcggcgg
    10681 tgatcgcctc gcagagggtc gacttgccgg tctcgttcgg ctccagcacg aggaggagct
    10741 caccgtcgcc ccaggaaagg tcgatcccct cgagcggccc gaagccctcg acgacgacgc
    10801 gctcgatcct cagaggtcct cctcggcagg cggcgccggc acccggccga ggagcgcctc
    10861 gcgccccagg acggccgcga gctcgaggac ccgtcgcgcc cgggggtcct ccgtcgcggc
    10921 gaggcgcgac tccagatcga ggacgaaccg tccttccgcc gtcccccggt cgggccgtgc
    10981 ctccccgtcg ccgagggtcc ggtcgcgcag cgccaggtgt gcgacgaggc ccttcaggtc
    11041 gccgagcgtc tgggtcgtcc gggtcgcggg ggcggaccgt ccgacgaccg tgagccgcac
    11101 gaggtcgccg ggcgtcgcgc cggcctcaag gaggagcgcg agggcggcgt cccgcaggcc
    11161 ctctccgtcc cggcccgccg cgtcgagctc gaggtcgagc accttccggc agtcggccgg
    11221 caacgtctcg acgcgggccg gcgcgccggc ctcgagcgtg accttcacga agtgcctcgg
    11281 cccggtctcg tccaggcccc ggcccgtcgg cgagccgctg taggccccgg ccgggctccc
    11341 gtcctcgcgc gtcacgacct ccatccggtg gtgatgcccc agggctgtcc agtcgaagcc
    11401 cgcaccgagg agctcctccc tcgagaacgg cgcggtcttc ttcgagccgg agggggcgtc
    11461 cgggccgcga tagctctcga gggagccgtg aagcatcagg atcgcgagct cggccgccgg
    11521 acgcgccggc gggggcgcca ggggccggcc ggtatcgacg aggggcgaaa ggaaagcccg
    11581 cccggtcacg gtcgcgtcgt cccgccccgg gacggccgcg gtctcccacg ccgagctccg
    11641 gaagacgcgg acgttaccgg gccagtccgg caggcccagc gcgtcgagga aggcgtcgtc
    11701 gtagaagcct ccgggcccgg cgaaatcgtg gttacccggg gcgacgaaga cggggacggg
    11761 agcgagggcc tcgagcgcct cgacgagcct ccggaagagc gtcgggggga cgctcccggc
    11821 gtcccagagg tccccgggca ggaggatggc gtcgggaccg gcctcgcgcg cggcccggac
    11881 gagacgctgt ggcgcctcgt cgacctcccg gcgccggagc gcccggagct cctctcccag
    11941 cccgaggccg cgcccggtga ggggcgagcc cacgtgccag tcggagctct ggaggagaac
    12001 gaggggcgcc acgacgatgg cgctcgggcg ccgcgcggcc tcaggccgcc gagccctggt
    12061 ccttgccgcc ctcggtgtcc gcgacgacgc ccgtgatggc gctcttggcc atcttcacct
    12121 tgagcgtgtc ggcgagcttc acccagacga tctggtcctc gacctgggcg acggtcccgt
    12181 agatcccgcc ggaggtcatg acgcggtcgc ccttcttcag ctgggcgagc atctccttcg
    12241 tcttcttctg ctgcttcctc atcggggcga ggaggacgaa gtagaagacg acgaagatga
    12301 ggaggaacgg gaccatctgg agcaggccgt ccttcatcga cggcgccgcc ggggcggcct
    12361 gcaggagggc gatcggattc atctcttgtc cttcccgaaa tcgaggcgcc cggcggcttc
    12421 ggccgcaaac gccgcgtagc gtcgtgcgga gagagcctcc cggattcccc gcatcaagtc
    12481 aaggatggcg tggaggttgt ggacggtcac gagcgtcgcc gccgtcggct ctccgagcat
    12541 gaagaggtgg cgcaggtagg cgcgcgacgc cgtcgcgcac gtcggacagg cgcacgccgg
    12601 gtcgagcggg gcccggtcgg tccggaagcg cgcctgcttg aggacgaccg tcccctccga
    12661 ggtgtaggcg aggccgtgac gcgcggcccg cgtcgggagg acgcagtcga agaggtcgaa
    12721 gccgctcgcg accgcgtgga ggaggtcgcc ggggtgcccg acgcccatca ggtagcgcgg
    12781 acgcccgggc ggaaggagcg gcccggtggc ctcgacgatc gcgcgcatct cctccttcgg
    12841 ctcccccacc gagacgccgc cgcacgcgat cccctcgaaa ccctccgcgg cgacggcggc
    12901 ggcgctctcg cggcgcaggt cggcgtacat ccccccctgc acgatgccga actgcgcgcc
    12961 ccggccgctc ccctcgcgcg ccgcgaggaa ggccgcccgc ccgcggcgcg cccaccggtg
    13021 cgtgagggcc atcgaacggg ccgcttcgtc cctcgtcgcc gggtacgggg tgcactcgtc
    13081 gagggccatc gcgacgtcga ccccgaagtc gcggagctgg agctcggcgg cccgctccgg
    13141 tgtcaggagg tgcgcgctgc cgtcgaggtg gctccggaag acgactcctt cctccgtcag
    13201 cttgcggttc gccgcgagcg acatcacctg gaaccctccg gagtcggtca ggatcggtcg
    13261 tccccagccg acgaacgcgt ggagaccgcc catctcgcgg atcagctccc cgccgggccg
    13321 gagcatcagg tgatacgtgt tcgcgaggac gatctcggcg cccgcggccg agacgtcgct
    13381 ccagcggacc gccttcaccg taccggccgt gccgaccggc atgaaggcgg gcgtctcgac
    13441 ggtcccgtgg tcgagcgtca cgcgacctcg ccgcgcggcc ccgtccacgg cgaggagctc
    13501 gaagctcagc acgtgcgcac cgcacgggcg aagcgccggg agagcgggag gaaggagcgg
    13561 ctcacacggc gagtgtaagc gccgcccgcc acgcctccgg atctcgttga ccgacctcgg
    13621 acccgatgct atgttccggc gtcttttttg agggaacgaa tgcccgatct cggccaaaaa
    13681 cacgagtgct tccgctgcgg gacgaagttc tacgacctga agagaactcc cgtcttctgc
    13741 ccgaagtgcg gagccgacca gaaggacgcg ccgccgcgcg acacgtcgcc ggccgcgctg
    13801 atcgcagctc gtcgcgccaa gcgcgacgag ttcgcagacc cggatctctt cgagaggaac
    13861 cgcgacgagg acgacctcgg cgcggagccc gaggacccga acgccaaggc ggccttggac
    13921 gacgaggagc ttcctctcgg agaggggccg agcctcgagg caggcgattc ggagagcgaa
    13981 gacgactact gacgggccgg ccgaggtcgc gatcggcctc ggcgggaacg tcggctctcc
    14041 acgcctcgcg ttcgaagcgg ccctcgcggg cctctcgtcc agcgtccacg gcctcgtcct
    14101 ctccccggtc taccggaccg cccccgtcgg cgggccgccc cagcccgact tcctgaacgc
    14161 cgtcgccgtc gggcggacga ggctcgagcc cgtcgcgctc ctcgcgaccc tcctgcgtct
    14221 cgaggcggag gcgggccgca ggcgctcccc gctccgcgac gggccgcggc cgctcgacct
    14281 cgacctcctc ctctacgggg acctcgtcct cgagctcccc gggctcgtcc tcccgcaccc
    14341 gaggatggcg agccggcggt tcgtcctcgc gccgctcgcg gacctccggc ccggcctcgt
    14401 cgtgccggga accggggcga cggtctcgga tctcctcgcc gcggccccgc cggcgcgggt
    14461 gcagcgagtc gcgtacgaaa acgcgtgagc ctcaggccgg cgcgtccggc cccctccccc
    14521 gcgcgggcga cattcgcggc tcgaaccgca gctgctcccc cgcgagcgtc acgcgcgtga
    14581 aagccggcac cgaggtcgtc agccagacgc tcccgccgat gacgctgccg cgcccgatca
    14641 cggtggagcc gccgaggatc gtcgcgcccg cgtagatcac gacgtcgtcc tccaccgtcg
    14701 gatggcgctt gacgccgcgg atcagctccc cgtgctcgtt cttcgggaaa gagagcgccc
    14761 cgagcgtcac cccctggtag atcttcaccc ggtccccgat cgtcgtcgtc tcgccgatga
    14821 cgacgcccgt cccgtggtcg atgaagaacg aacgtccgat cgtcgcgccg gggtggatgt
    14881 ccacccccgt ctcgttgtgc gcgaactcgg tcatcaccct ggggatcagc cgcaccccca
    14941 tccggtgcag ctcgtgcgcg atccggtagg tgaagatggc cttcacgccc gggtaggcga
    15001 ggatcagctc gtcgaacccc tgggccgcag ggtcgccgtc gtaggcggcc tggacgtcct
    15061 cggcgacgcg gtcggcgacc tcgggcatcc gctcgaacag ctcggtgacg acctcccgcg
    15121 cgtggcggcg cgcggccggc cggccctcgg aggcctcgag cgccttctcg acctccgcgg
    15181 agagccggag gtgaatccgg aacaggacgt cgccgaggtg gtcgcggagg ttgtcccggt
    15241 agaggaccgt atctccgaag tacccgggat agacgacctc ctggaagagc cggaggatct
    15301 cgatgatctc cttcttctgg ggacgggggg cggcctccac cgcggagagc tgcgggcgac
    15361 tctcggcggt cgcgacgatg cgcgccatcg cgccccggat ccgctccttc atcgccgtcc
    15421 cttcccggcc cacgcggcca ggagggcacg cgcgtcctcc tgcgcccggg cggcctccac
    15481 ctcgaggccc ggcgtcggcg cttcgcggac gagcgcctcg agcatcccgc gcgcctcgtc
    15541 cttcttcccc ttctcgtagt cccagatcgc ctcggcgagg tagagacggt tgacgaagtt
    15601 ccgcggcccc ttctccacgg cgcgccggag gctcgcgagc gcctccgccc gggacgccca
    15661 gccggtgacg aaggggatcg acggcgtctg gtggtggagc cggccgagga cgcggtaccc
    15721 gcctccttcg tcccacgtcg ggtcgagcgc cacgaccgcg gcagcgtagt cgcggatctt
    15781 cgcggccgca ccctgccggg ccgccgccgt cttgccgaag acgagcgccc atttccccca
    15841 gttcaccccg gcccagagga acgccgggac ggcgtccggc gtctgtgcga gatacggtgc
    15901 cagctcgacc ggcgaggcgc tcgtgaggtc ccggcccgcc gacgctcccg cggttcgccg
    15961 gaggagcgcg agcgtctctt caccgacccg cttcccctcg tcgaagacgg ccttcttggc
    16021 cgcctccccc ggcgtggcgt gctccccgag gaagtagagc gcccgcatca gccgccaccg
    16081 ggcaccgacg gactcgggct cggcggcgac ggcggcgcgg gccgcggcga cgacgtcccg
    16141 ggcggccgca tccgcggcgg tcccgcccgt cgccccggcg gcccggcgcg cccaggcggc
    16201 gtcggccgcc gcgaccgtcg cggatgggtc cgccgcgagg acggacgccg gaccgaggag
    16261 gaaagagacc aggatcacgg aggccggaac gccccgggtc gtacgctgca tcttcagatc
    16321 ttacgttcgt cgcgacccgc aggacccgtc ccgcgcgcat gccggcggac ggctagaatg
    16381 gcatccttct tgcgcatccc gggaggatgc gcatcgtgag gtgtctcaat ggctgaagcc
    16441 ccgccaaagg gcgagggggc cgctcccccg gccctgcagt tctcgatgga cgactccgtc
    16501 gcgagcggcg tctacgcgaa cttcgtcaac atcatccaca acccggccga gttcgtcttc
//
LOCUS       DAOONL010000076        16441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3717_length_16441_cov_14.777371, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000076 DAOONL010000000
VERSION     DAOONL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3717_length_16441_cov_14.777371"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1700)
                     /gene="tkt"
                     /locus_tag="PLP50_11555"
     CDS             complement(<1..>1700)
                     /gene="tkt"
                     /locus_tag="PLP50_11555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPA52227.1"
                     /translation="DALRRLAANTLRTLAMDGVQKANSGHPGMPMGMADVATVLFTEF
                     LKFSPKSPSWLDRDRFVLSAGHGSMLLYGLLHLSGYDLPLDDLKAFRQWGSRTPGHPE
                     HGLTPGVEVTTGPLGQGISTAVGLALAERMLAARFNREGHEIVDHFTYAIASDGDLME
                     GVSHEACALAAHLGLGRLVVLYDDNGISIDGPTSLSFSEDVPARFAAYGWHVARVDGH
                     DPDAVSAAVAAAKAEGGKPSLIACRTHIGFGSPNRQDTSKAHGEPLGADEIRLTKEKL
                     GWPADAQFLVPEEVRAFFAAAAARGAKAEADWNARLAACRAAHPDLAKELDLFLEGRL
                     PEGWEAKLPAFGAEKKLATRQASGAVLEALVPAIPTLVGGSADLTPSNNTRAKAAVDV
                     KAGEYAGSYLRFGIREHGMGAILNGLALHGFRPFGGTFLVFADYMKGAIRLASLMEQP
                     VVYVFTHDSIGLGEDGPTHQPIEQLASLRATPGLVVYRPADANETAAGWRVALSRTHG
                     PTALALTRQGLPVLPPNEGAMKGGYVVADADGFRVILIGSGSELHLALAAQKLLAADG
                     "
     assembly_gap    1701..1800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1801..2101)
                     /locus_tag="PLP50_11560"
     CDS             complement(<1801..2101)
                     /locus_tag="PLP50_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52228.1"
                     /translation="MEAWTAIGPIVARRPGGGEVEPVEVGAAGYVRRRARWRPRAARA
                     CTIRRRLGPLPVRRPALRGAPAGGIPRGPGAKEIPMADDALRRLAANTLRTLAMDG"
     gene            2082..2921
                     /gene="kdsA"
                     /locus_tag="PLP50_11565"
     CDS             2082..2921
                     /gene="kdsA"
                     /locus_tag="PLP50_11565"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPA52229.1"
                     /translation="MAVHASITPYLSAGDRCRPVFFAGPCVIESRTHTLKMAKAIKGV
                     FEAAGVADRLVFKASFDKANRSAGDSFRGPGIEKGLDVLSAVKERLDLPILTDVHEAE
                     QCELAAEVADVLQVPAFLSRQTDLLVAAARTKRAVNVKKGQFLAPDDCAQIVAKVRGA
                     GNPNVLLTERGSTFGYHNLVVDFRSLPMMRALGAPVIYDATHSLQLPGGLGKETGGAR
                     QYAPALARAAAAVGVDGYFLEVHDNPANAKSDRATQLPLADLPKFLFALLKFDALRRE
                     TEG"
     gene            2925..4445
                     /locus_tag="PLP50_11570"
     CDS             2925..4445
                     /locus_tag="PLP50_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPA52230.1"
                     /translation="MRPARAGALLLTAALLAPAASGAEVRLQSVAFPEGSRVDVPFAG
                     TGRAPTGATVEAEVRAEGTRTRIALAWKRLPPALLFGGDVTAYVLWGVATNGTVEHLG
                     ELFPREPKGNAEFATGRKSFALMVTAEPYPGVARPSELVVFTGEAPKPEKARSEPFTF
                     TQLSAGAMPSTPSIATAEWTSREPLELLQARAVLARAQRLKEGDADQSTVREARFALA
                     EAESAARGGRVSAPLAEARRATALASEAIREVERRRAAEEAARLEAEARAKEAARKAR
                     AEDEAERRRQAEAALAEIEELRRQAASDLQQTRQASAALELARAQLEEERSLLRERQT
                     LLEREREELSTRLADALERVAPTARSERGLVVSLSGASFESGRATLTPAARVTIGKLA
                     GILLMSPGSSVRVEGHTDSSGSAAANRKLSSERARNVADLLREHGIDGDRIAFEGFGA
                     DRPVAPNATASDRARNRRVEVVLTGAAVEEKPGALSPVPLPDGPAPPADEVPPPLR"
     gene            complement(4462..4836)
                     /locus_tag="PLP50_11575"
     CDS             complement(4462..4836)
                     /locus_tag="PLP50_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HPA52231.1"
                     /translation="MTAAERRVAREWKTIQAMIRIYCRNLHGRRGALCEECESLRLYA
                     ERRLEKCPFAEEKPTCVKCPVHCYEAGMRERVRQVMRYSGPRMLLWHPILAIRHLRDE
                     KLPLSAKAQKVAERLAKQASGG"
     gene            complement(4833..5195)
                     /locus_tag="PLP50_11580"
     CDS             complement(4833..5195)
                     /locus_tag="PLP50_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52232.1"
                     /translation="MKSVLRALPVVLSALVLAAHFYRSRSLALVALSLALPLLLLVKE
                     RWSARVVQAGLVLGALEWVRTLAFFAGQRMEAGRPWARLAVILGVVAVLTALSALAVK
                     VPRAAVPPATGREPEAAS"
     gene            complement(5534..5713)
                     /locus_tag="PLP50_11585"
     CDS             complement(5534..5713)
                     /locus_tag="PLP50_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52233.1"
                     /translation="MFWDVSIVVVTPVWACWLKTFRTCGEAKTSTEPNGRKERFRERS
                     VLQTSSVLKASRAVK"
     gene            6034..6669
                     /locus_tag="PLP50_11590"
     CDS             6034..6669
                     /locus_tag="PLP50_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA52234.1"
                     /translation="MGTTERREREKERVRGRILDAARELFARDGYDAVTMRKIAEAVE
                     YSPTAIYLHFKDKESLVRELCIADFDSLARSFQRIAREPDPLERLRKAGLAYADFAVE
                     HPNHYRLMFMTPHPEGTKDDPSAAGRKGNPEADAYAFLVATVREAIEKDLLRPDLKDP
                     NLVAQAAWAGVHGVVSLHLAKAHDAWVDWRPLKKTVALVVDAFTRGVAKEG"
     gene            6672..7799
                     /locus_tag="PLP50_11595"
     CDS             6672..7799
                     /locus_tag="PLP50_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52235.1"
                     /translation="MVSLARRNLLHDRLRFAITIAGVAFAVTLVFVQVGLFLGLLENA
                     SVTIEHTGADLWVTSRNTPNVDFAHTFPETLVQRVRSVPGVARADNLLVWFMQISLPT
                     GTQEGTLVYALEDFAAWDLPWSVKEGRLEDLPRGDYLFLDDSATRRFGPFAAGEYREV
                     NERRLKIIGRTVAARSFTTTPISFVDYGLAQELGSQTLRGKTTYVLVKLAPGADLEAV
                     RAEIRRRLPYNDVFTKAEWAKRSRSYWVANTGIGLNMYLTVFLGVLVGVVVVALTLYT
                     STMEHLKEFGTVKAIGGSNRDIYRILAEQSVIAAVLGFAAGALFALAAAPGMAKAGLQ
                     LIITPGFGAAVLVGTVLLCLAASLVSFRKVAALDPALVFRG"
     gene            7860..8531
                     /locus_tag="PLP50_11600"
     CDS             7860..8531
                     /locus_tag="PLP50_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52236.1"
                     /translation="MTVLSSRNVVKRFTEGKQTVEVLKGVSLEARRGEIVALEGPSGS
                     GKTTFLSILGCILTPTEGRVVVDGHEVDASRPGELPAIRRRSIGFVFQQFNLFPALTA
                     LENVEYSLNVRGRKGPAARKEAERVLDAVGLADRTSFLPRDLSGGQKQRVAIARALAG
                     APPLLLADEPTANLDSVAGRQVLETFRDLAKREDRALVIVTHDPQVRTIADRVAVIRD
                     GLLAA"
     gene            8552..9430
                     /locus_tag="PLP50_11605"
     CDS             8552..9430
                     /locus_tag="PLP50_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52237.1"
                     /translation="MTTKKLLTFSIPVAAFLFAGIRAARHAGDAPPQAPERPLALASA
                     ASRVVAEGRVVTYPGRQVVVGTDFAGTLERVAVEEKQRVERGAQLAVVRSDEERAALA
                     QAKARFAEAEADARLALAEVERARRLYAGKVGARQAVDRAERDRDAAAARRETARAEI
                     ERLAALVAKARVLSPISGVVLEKHVSTGETVERGSRVATIADLDRLRVEAEVDEADTA
                     RVALGAPVLVRAEGESTTWTGTVEEIPDAVTGRKIRPQDPARPTDTRVLIVKVALASQ
                     EGLKLGRRVEVEIRGR"
     gene            complement(9457..10146)
                     /locus_tag="PLP50_11610"
                     /pseudo
     CDS             complement(9457..10146)
                     /locus_tag="PLP50_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            10286..10696
                     /locus_tag="PLP50_11615"
     CDS             10286..10696
                     /locus_tag="PLP50_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPA52238.1"
                     /translation="MTPRGLHVDPRDAVPIWKQIEEGMRRLVASGALAPGAAVPSVRD
                     LARDLQVNPATVVRAYQRLVEAGLFAVRRGEGTFVAEAPPSLPKPERTRALAEGALRF
                     ASTAVTVGASRDEAARALEGAFARLAPPKEGEKK"
     gene            10693..11613
                     /locus_tag="PLP50_11620"
     CDS             10693..11613
                     /locus_tag="PLP50_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52239.1"
                     /translation="MSATAAARTERLTVRYGLTTAVDAVSLDVPKGSVFALLGRNGAG
                     KSSLLRCLLGWQKATGGRCELFGEDPWNRRARLMERVGVVPEEPDAPREMTPRQLGAF
                     CARLYPSWDAKGYEARLSRFGVPQATPFVRLSKGQKGLTQLSLALASSPDLLLLDDPT
                     LGLDPVARHAFFDEIVAELADRGTTVFLTTHDLAGVERIADRVGILKDGRLLLLEETE
                     ALKSRFRTVRYRSEVTEGRTDFGTELDELEPVRVKVRGWGIEAVVSAFSDERFERFAA
                     KDGIVDAEAAPLSLEELFLAVAGEGKDTIR"
     gene            11610..13577
                     /locus_tag="PLP50_11625"
     CDS             11610..13577
                     /locus_tag="PLP50_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52240.1"
                     /translation="MKRFAAVALREVAERRSVLVAAAAAAILPFLVPLLPSVPGSDAA
                     TARALTALVLACAFGAGGAVLVGASVVGRELAEKRLSFHFARPLSAPVIWGGKVVGGL
                     ALVLLAELFVYLPASIASGRFPGLVDRFDAPAMAALLLLAVPLFLIAWIASVAVRSRS
                     PWLVVDFVLLAASAAVVWSLGKRLLLHGQAPTLMETAVGAVLLLTILGAATFVQVCAG
                     RTDARRGHGAQSVVLWGGLGIFLLGLAVWVDGKVDPGYETFVHLAAEPTGSSGEWILL
                     TGTPKTVDDFRRARYLVDLRTGRSVLLPLSFLHTVSADGSRATWIGPFGPFDKSPRRT
                     VADLRSGDSTEYPIGGLGDGASGPSFELALSADGSRLAFLSAEGVCDVTDLATGRLLA
                     SARVPGIERWFRQVSFDSRDVVRIEPRTRMAEEMGHAHELAPLPPAPALFLLDVPSRT
                     LKPVGTALAPAPFFQRVVGPTTTPRAFVRRADDRRRLHLVDLMNGAALASFGSAETKG
                     GHVQGGSLADGRLFVKVDDPGTASRIVLLSPDGTTLREVNLPDDAGGWDVGLEAAPGV
                     LPIARPEWLRKEEPHSLLVDLNTGQTRSLGRLRLGVRFGLSSVVSPVVPPPVGSPATR
                     LFRDADTGRLVLLDPQTQERKPLTRGRPSGK"
     gene            13588..14556
                     /locus_tag="PLP50_11630"
     CDS             13588..14556
                     /locus_tag="PLP50_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPA52241.1"
                     /translation="MTTPARREARRVLEIEAAAIRSLADSLDGGFDTAVALLHGTTGR
                     VVVTGIGKSGIVGQKIAATLASTGTPAFFLHPAEAIHGDLGMILAGDVVLALSHSGET
                     EEVVALLPHVRRRGAKLVALTGRRASTLGKEADAVVEAAIREEACPLNLAPTASTAAQ
                     LALGDALAMALSVEKGFRPEDFAALHPGGKLGKRFLRVSDLMHAGSDVPLVPLDAPMK
                     DVVYEMSKKRLGITGVVDGDGRLAGVVSDGDLRRLLEREGEKAWSADAAFAMNRTPKT
                     IAAGAFATEALRLMEEKKITSLFIVDGDGRPGGIVHLHDLWGVESI"
     gene            14589..15194
                     /locus_tag="PLP50_11635"
     CDS             14589..15194
                     /locus_tag="PLP50_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPA52242.1"
                     /translation="MPAAPIVPDDLRTKLLRVRALVLDVDGVLTDGTLTFDEDGRELK
                     SFDVKDGLGLVLLRDLGYRIGLISSRVSKVVALRCRDLKIPEECVMQGELDKASAFDR
                     LLGKWGFEAFEAVAVGDDLPDLGMMRRAGIGACPSDAVTIVKDAAALHLTKPGGGGAV
                     RELCDLILEIRRGARADKPQEVKPPQASSDGSVVPFPGSRG"
     gene            complement(15304..16059)
                     /gene="tatC"
                     /locus_tag="PLP50_11640"
     CDS             complement(15304..16059)
                     /gene="tatC"
                     /locus_tag="PLP50_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPA52243.1"
                     /translation="MTDAPPDDLTRMTFLEHLEELRKRLLWSVIAVAAGFFLSWWKAQ
                     DLFRIAQKPILEVLPAGTKLAYTNLTEPFMLYLNIALIAGIFLASPVILYQVWLFVAP
                     GLYRHEKRWVVPFVFFAALSFCGGGWFGYAVAFPMVAQFLVTMGADFTPVLKIDDYLS
                     ILSKILLGMGLVFQTPIVIVFFVRIGVVTEKWLLQKFRYAVLVIFLIAALITPTPDIP
                     TQCAFALPMIALYLLGIFLAWIVGKRKKTEPAV"
     gene            complement(16056..16334)
                     /locus_tag="PLP50_11645"
     CDS             complement(16056..16334)
                     /locus_tag="PLP50_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPA52244.1"
                     /translation="MGNLGMPELIFIFALALLILGPKKLPELGKQLGKGLGEFKRASN
                     DLKRSIEEEVEKAARDEAPKRDAAPQAVPAEGTVASAGEERSDPIRPA"
BASE COUNT         2292 a         6102 c         5686 g         2261 t
ORIGIN      
        1 atcccgtcgg ccgcgaggag cttctgcgcg gcgagggcga ggtggagctc ggagccggag
       61 ccgatcagga tcacgcggaa gccgtccgcg tccgcgacga cgtagccgcc cttcatcgcc
      121 ccctcgttcg gcgggaggac cgggagcccc tggcgcgtga gcgcgagcgc cgtcgggccg
      181 tgggtccgcg agagggcgac gcgccagccg gcggcggtct cgttcgcgtc ggcggggcgg
      241 tagacgacga ggcccggcgt cgcccggagg gaggcgagct gctcgatcgg ctggtgggtc
      301 gggccgtcct cgccgaggcc gatcgagtcg tgcgtgaaga cgtagacgac cggctgctcc
      361 atgagcgacg cgaggcggat cgcccccttc atgtagtcgg cgaagacgag gaacgtcccg
      421 ccgaacgggc ggaagccgtg gagggcgagg ccgttgagga tcgcccccat cccgtgctcg
      481 cggatgccga agcggaggta cgagccggcg tactcccccg ccttcacgtc gaccgccgcc
      541 ttcgcgcgcg tgttgttcga cggcgtcagg tcggccgagc cgccgacgag cgtcgggatc
      601 gccgggacga gcgcctcgag gacggcgccc gaggcctggc gcgtggcgag cttcttctcc
      661 gccccgaagg ccgggagctt cgcctcccac ccctcgggga gcctcccctc gaggaagagg
      721 tcgagttcct tcgcgaggtc ggggtgcgcc gcgcggcacg ccgcgaggcg cgcgttccag
      781 tcggcttccg ccttcgcgcc ccgcgcggcc gcggcggcga agaaggcgcg gacctcctcg
      841 gggacgagga actgcgcgtc cgccggccag ccgagcttct ccttcgtcag ccggatctcg
      901 tcggccccga gcggctcgcc gtgggccttg gaggtgtcct ggcggttggg gctgccgaag
      961 ccgatgtgcg tccggcaggc aatgagcgag ggcttgccgc cctcggcctt cgcggccgcg
     1021 acggctgcgg agacggcgtc ggggtcgtgg ccgtcgacgc gcgcgacgtg ccagccgtag
     1081 gcggcgaagc gggcggggac gtcttccgag aaggagagcg acgtcgggcc gtcgatcgag
     1141 atgccgttgt cgtcgtagag gacgacgagc ctgccgaggc cgaggtgcgc ggcgagcgcg
     1201 cacgcctcgt gcgagacccc ttccatcagg tcgccgtcgc tcgcgatcgc gtacgtgaag
     1261 tggtcgacga tctcgtgacc ttcgcggttg aagcgggccg cgagcatccg ctcggcgagc
     1321 gcgaggccga cggccgtcga gatgccctgt ccgagcggtc cggtcgtcac ctcgacgccg
     1381 ggcgtcaggc cgtgctccgg gtggcccggc gtccggcttc cccactggcg gaacgccttc
     1441 aggtcgtcga gcgggaggtc gtacccggaa aggtggagga ggccgtagag gagcatcgac
     1501 ccgtgccccg ccgagaggac gaaccggtcg cggtcgagcc acgacgggct cttcgggctg
     1561 aacttcagga actcggtgaa gaggacggtg gccacgtcgg ccatccccat cggcatcccg
     1621 gggtgcccgg aattcgcctt ctggacgccg tccatcgcga gcgtccggag ggtgttcgcc
     1681 gcgaggcggc ggagggcgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 cgccgtccat cgcgagcgtc cggagggtgt tcgccgcgag gcggcggagg gcgtcgtcgg
     1861 ccatcggaat ctccttcgcg cccggccccc gggggattcc tccggcgggc gcgccccgga
     1921 gtgcggggcg gcggaccgga agggggccga gccttcggcg gatcgtacat gcccgcgcgg
     1981 ctcgcggccg ccaacgcgcc cggcgccgga cgtacccagc ggctccgacc tcgaccggct
     2041 cgacctcgcc gccgcccggc ctccgggcta cgatcgggcc gatggccgtc cacgcctcca
     2101 tcacccccta cctctccgcc ggcgaccgct gccgccccgt cttcttcgcc ggaccgtgcg
     2161 tcatcgaatc gcgcacccac acgctgaaga tggcgaaagc gatcaagggc gtcttcgagg
     2221 ccgcgggcgt cgccgaccgg ctcgtcttca aggcgtcgtt cgacaaggcg aaccgctccg
     2281 cgggcgactc cttccgcggc cccgggatcg agaaagggct cgacgtcctc tcggccgtga
     2341 aggagaggct cgacctgccg atcctgaccg acgtccacga ggccgagcag tgcgagctcg
     2401 cggcggaagt cgccgacgtc ctccaggtgc cggcgttcct cagccgccag acggacctcc
     2461 tcgtcgcggc cgcgcggacg aaacgcgccg tgaacgtgaa gaaggggcag ttcctcgccc
     2521 ccgacgactg cgcgcagatc gtcgcgaagg tgcgtggcgc cgggaacccg aacgtcctcc
     2581 tcacggagcg tgggtcgacg ttcggctatc acaacctcgt cgtcgacttc cgctccctcc
     2641 cgatgatgcg ggcgctcggc gccccggtga tctacgacgc gacgcactcc ctccagctcc
     2701 cgggcggcct cgggaaggag acgggcgggg cgcggcagta cgcgccggcg ctcgcgcgcg
     2761 ccgcagcggc cgtcggcgtc gacggctact tcctcgaggt ccacgacaac ccggcgaacg
     2821 cgaagtccga ccgggcgacg cagctcccgc tcgccgacct tccgaaattc ctcttcgccc
     2881 tcctgaagtt cgacgcgctc cggcgggaga cggagggctg aggcgtgcgc cccgcccggg
     2941 caggcgccct cctcctgacc gccgcgctcc tcgcgccggc cgcgtccggc gcggaggtcc
     3001 gcctccagtc ggtggctttc cccgagggga gccgtgtcga cgtccccttc gccggcaccg
     3061 gccgcgcccc caccggcgcc acggtcgagg cggaggtccg ggccgaaggg acccggaccc
     3121 ggatcgcgct cgcctggaag cggctgccgc ccgccctcct cttcggcggc gacgtgaccg
     3181 cctacgtcct ctggggcgtc gcgacgaacg ggacggtcga gcacctcggc gagctcttcc
     3241 cccgcgagcc gaagggaaac gccgagttcg cgaccggccg gaagtccttc gccctgatgg
     3301 tcaccgcgga accctacccg ggcgtcgcac ggccttcgga gctcgtcgtc ttcaccggcg
     3361 aggccccgaa gccggagaag gcgcgcagcg agccgttcac gttcacgcag ttgtcggcgg
     3421 gggcgatgcc gtcgacccct tccatcgcga cggcggaatg gacgagcagg gagccgctcg
     3481 agctcctcca ggcccgagcc gtgctcgcga gagcccagcg gctgaaggag ggcgacgccg
     3541 accagagcac cgtccgcgaa gcccgcttcg cccttgccga ggccgagagc gcggcccgcg
     3601 gggggcgcgt ctccgctccg ctcgccgaag cgcgaagggc caccgcgctc gcctccgagg
     3661 cgattcgcga ggtcgaacgg cgacgggccg ccgaagaggc cgcccggctc gaggccgaag
     3721 cgagggcgaa ggaggcggcc cggaaggccc gcgccgagga cgaggccgag cgccgccgcc
     3781 aggcggaagc ggccctggcc gagatcgagg agcttcgccg gcaggcggcc tccgacctcc
     3841 agcagacgcg acaggcctcc gccgccctcg agctggcccg ggcgcagctc gaagaggagc
     3901 ggtcgctcct gcgcgagcgg cagacgctcc tcgagcgcga gcgcgaggag ctctccacgc
     3961 gtctggccga cgcgctcgaa cgcgtcgcgc cgacggcgcg gtcggagcgc ggtctcgtcg
     4021 tgagcctctc cggcgcctcg ttcgagagcg ggcgggcgac cctcacgccg gccgcgcggg
     4081 tcacgatcgg gaagctcgcg ggaatcctcc tgatgagccc cggctcgagc gtccgcgtcg
     4141 agggtcacac cgattcctcc ggcagcgccg ccgcgaatcg gaagctctcg tcggagcgag
     4201 cccggaacgt cgccgacctc ctccgcgagc acgggatcga cggcgatcgc atcgcgttcg
     4261 agggcttcgg cgcggaccgc cccgtcgcgc cgaacgcgac cgcatcggac cgggcgcgga
     4321 acaggagggt cgaggtcgtc ctgaccgggg cggcggtcga agagaagccc ggagcgctct
     4381 cccccgtgcc gcttccggat ggccccgctc cgccggcgga cgaggtccct ccgccgctcc
     4441 ggtaaggaga cccccgggcc ctcagcctcc ggacgcctgc ttcgcgaggc gctccgcgac
     4501 cttctgcgcc ttcgcggaga gcgggagctt ctcgtcccgg aggtgccgga tcgcgaggat
     4561 cggatgccag aggagcatcc gggggccgga gtagcgcatc acctggcgga ccctctcccg
     4621 catcccggcc tcgtagcagt ggaccgggca cttcacgcag gtcggcttct cctccgcgaa
     4681 cgggcacttc tcgaggcgtc tctccgcgta gagccggagc gactcgcact cctcgcaaag
     4741 ggcgccccgc cggccgtgga ggttccggca gtagatccgg atcatcgcct ggatcgtctt
     4801 ccactcccgg gccacgcggc gctcggctgc ggtcacgagg cggcctccgg ctccctcccg
     4861 gtggccgggg gaaccgccgc gcgcgggacc ttcaccgcga gggccgagag cgccgtcagc
     4921 acggccacga ccccgaggat cacggcgaga cgagcccagg ggcgcccggc ctccattctc
     4981 tgacccgcaa agaaggccag ggtccgcacc cactcgagag cgccgaggac gaggccggcc
     5041 tgaacgaccc gggcgctcca ccgctccttc acgaggagga ggagcggaag ggccagcgat
     5101 agcgcgacca gggcgaggct ccgcgaccgg tagaagtggg cggcgaggac gagagccgag
     5161 aggacgacgg ggagggcccg gagaacgctc ttcatcgctc cacgaggaga ccacggctca
     5221 cggcctcccg ccatgacggc ggtcatgccg gtccgatcgg gcgcgcccat ccggagaccg
     5281 gtcacacggt caatggcagg ctccttgctg ctcttggcgt tcaaccaggt taaccagggc
     5341 aaggccgacc ccgcaatccg ctccgcgagc gcctcgggct cacggacgcg gggatcgggc
     5401 ggtgacgacg gattcggatt tccgggtcgc agaacttcga ctcgggcctt ccgtacggag
     5461 ccgtcggcgc cgtcgacacg cggccctacg tcacgaaccc gggctacctg agcgtcccgt
     5521 cgtcccacac ctactatttc acggcgcgcg acgccttcag gaccgacgac gtctggagga
     5581 ccgacctctc cctgaacctc tccttccggc cgttcggctc cgtcgaggtc ttcgcctcgc
     5641 cgcaggtcct gaacgtcttc aaccagcagg cccagaccgg cgtcaccacc acgatcgaga
     5701 cgtcccagaa cacgaccggc tacacggcct tcaacccctt cacgaccgcc cccgtccagg
     5761 gcgcgaggaa caccggcgcg aactggaact actccgccgc gttcggctcc cccgtcagcc
     5821 cggcgagcta tcagctcccg aggaccttcc gcttctcggt cggcctccgc ttctagcgtc
     5881 ccgcccggag cgccggccca cccggcgctc cggccttccg gaggagcccc gcccatcggg
     5941 ccggggcttt ctttctctcc tggacccttg acagcacgga acgccgttcc catacttgac
     6001 tgcgttaagt taggtaacga ggttcagtaa actatgggaa ccaccgagcg gcgcgaacgg
     6061 gagaaggagc gagtcagggg gcggatcctc gacgccgccc gggagctctt cgcccgcgac
     6121 ggctacgacg ccgtcacgat gcgaaagatc gcggaggcgg tcgagtattc cccgacggcg
     6181 atctacctcc acttcaagga caaggagagc ctcgtccgcg agctctgcat cgccgacttc
     6241 gactccctcg cccgctcctt ccagaggatc gcccgcgagc cggacccgct cgagcggctg
     6301 cgaaaggctg ggctcgccta cgccgacttc gcggtcgagc acccgaacca ctaccggctc
     6361 atgttcatga cgcctcaccc ggaagggacg aaggacgacc cctcggcggc cggccggaag
     6421 ggaaaccccg aagccgacgc ctacgccttc ctcgtcgcga cggtgcgcga ggcgatcgag
     6481 aaggacctcc tccgccccga cctgaaggac ccgaacctcg tcgcccaggc ggcctgggcc
     6541 ggcgtccacg gcgtcgtctc gctccacctc gccaaggcgc acgacgcctg ggtcgactgg
     6601 cggccgctga agaagaccgt cgccctcgtc gtcgacgcgt tcacccgcgg cgtcgcgaag
     6661 gagggctgac cgtggtctcg ctcgcccgga ggaacctcct ccacgaccgg ctccgcttcg
     6721 ccatcacgat cgccggcgtc gccttcgccg tcacgctcgt cttcgtccag gtcggcctct
     6781 tcctcggcct cctcgagaac gcctcggtga cgatcgagca cacgggggcc gacctctggg
     6841 tcacgtcccg aaacaccccg aacgtcgact tcgcccacac cttccccgag accctcgtcc
     6901 agcgggtccg ctccgtcccg ggagtcgcgc gggccgacaa cctcctcgtc tggttcatgc
     6961 agatcagcct ccccaccggg acgcaggagg ggacgctcgt ctacgccctc gaggacttcg
     7021 ccgcctggga cctcccctgg agcgtgaagg agggtcgcct cgaggacctc ccgcgaggcg
     7081 actacctctt cctcgacgac tccgcgacgc gccgcttcgg gccgttcgcc gccggcgagt
     7141 accgcgaggt caacgagcgc cgcctgaaga tcatcggccg gaccgtcgcc gcccgctcgt
     7201 tcacgacgac gccgatctcg ttcgtcgact acggcctcgc gcaggagctc ggatcgcaga
     7261 cgctccgcgg aaagacgacc tacgtcctcg tgaagctcgc cccgggggcc gacctcgagg
     7321 ccgtccgggc ggagatccgc cgccgcctcc cgtacaacga cgtcttcacg aaggccgagt
     7381 gggcgaagcg ctcgcggagc tactgggtcg cgaacaccgg catcggcctg aacatgtacc
     7441 tcaccgtctt cctcggggtc ctcgtcgggg tcgtcgtcgt ggcgctcacg ctctacacct
     7501 cgacgatgga gcacctgaag gagttcggga cggtgaaggc gatcggcggg agcaaccggg
     7561 acatctaccg gatcctggcc gagcagtcgg tcatcgccgc cgtcctcggc ttcgcggcgg
     7621 gcgccctctt cgccctcgcg gcggcgcccg gcatggcgaa ggccgggctg cagctgatca
     7681 tcacccccgg cttcggggcg gccgtcctcg tcgggacggt cctcctctgc ctcgccgcgt
     7741 ccctcgtgtc gttccgcaag gtcgccgccc tcgacccggc gctcgtcttc cgcggctgaa
     7801 gccgcttctc ccgtctcttc gcgggcctct gccggaggcc cccgtgcaag gagtctccga
     7861 tgaccgtcct ctcgtcccga aacgtcgtca agcggttcac cgaagggaag cagaccgtcg
     7921 aggtgctgaa gggggtctcc ctcgaggcgc gccgcggcga gatcgtcgcc ctcgagggcc
     7981 cctcgggctc cgggaagacg accttcctct cgatcctcgg ctgcatcctg acgccgacgg
     8041 aagggcgcgt cgtcgtcgac gggcacgagg tcgacgcgag ccgccccggc gagctcccgg
     8101 cgatccgccg ccgctcgatc ggcttcgtct tccagcagtt caacctcttc cccgccctca
     8161 cggcgctcga gaacgtcgag tactcgctga acgtgagggg ccggaagggg cccgccgcgc
     8221 ggaaggaggc cgagcgcgtc ctcgacgccg tcggcctcgc cgaccggacg tcgttcctcc
     8281 cgcgggacct ctcgggcggg cagaagcagc gggtcgccat cgcgcgggcc ctcgccgggg
     8341 cgccgcccct cctcctcgca gacgagccga ccgccaacct cgactccgtc gcggggagac
     8401 aggtcctcga gacgttccgc gacctggcga agcgggagga ccgcgccctc gtaatcgtca
     8461 cccacgaccc gcaggtccga acgatcgccg accgagtcgc cgtcatccgg gacggcctcc
     8521 tcgccgcctg acgcccccgg gaggtccgaa catgaccacg aagaagctcc tcacgttctc
     8581 catcccggtt gccgcgttcc tcttcgccgg gatccgcgct gcccggcacg cgggcgacgc
     8641 gccgccgcag gctccggaac ggcccctcgc gctcgcgtcc gcggcctccc gcgtcgtcgc
     8701 ggagggacgc gtcgtgacct accccggccg gcaggtcgtc gtcgggaccg acttcgccgg
     8761 cacgctcgag cgggtcgccg tcgaggagaa gcagcgcgtc gagagaggcg ctcagctcgc
     8821 cgtcgtccgc tcggacgagg agcgggccgc cctcgcccaa gcgaaggcgc gcttcgcgga
     8881 ggccgaggcg gacgcccggc tcgccctcgc cgaggtcgag cgcgccaggc ggctctacgc
     8941 ggggaaggtc ggggctcgcc aggccgtcga ccgcgccgag cgggaccggg acgccgccgc
     9001 cgcgaggcgg gagacggccc gggccgagat cgagcgactc gcggccctcg tcgcgaaggc
     9061 gcgcgtcctc tcgcccatct cgggcgtcgt cctcgagaag cacgtctcca ccggcgagac
     9121 cgtcgagcgc ggctcgcggg tcgcgacgat cgcggacctc gaccggctcc gggtcgaggc
     9181 ggaggtcgac gaggcggata ccgcccgcgt cgcgctcggc gcgcccgtcc tcgtgcgggc
     9241 cgagggggag agcaccacgt ggacggggac ggtcgaggag atccccgacg cggtcacggg
     9301 ccggaagatc cggccccagg acccggccag gccgaccgac acgcgcgtcc tcatcgtgaa
     9361 ggtggcgctg gcgagccagg agggcctgaa gctcggccgg cgcgtcgagg tcgagatccg
     9421 gggccggtag agctcccgcc gccggaccgg cggccggccc acgtgcttca gcgcctcgcg
     9481 ccgcgagagc ggcgagaggc gctccgcgtt cgcggcgacg tatcgggaga cctccgtggg
     9541 gtcggcccgg gcgtactgcc ggagcgccca cccgatcgcc ttccggagga agaactcctt
     9601 cgaggcgagc gagggctcga tgcacgcgaa gagcagcccg gggtcggtct cgccccggga
     9661 gccgagctgg cagaggatcg ccgagcggcg cttccagagg tcgtcgctcc gagcccaggc
     9721 gagcatcgcg cgcctcgtcg cgcgcggctc cttcgcgagg agctccccga ggaggtgcgc
     9781 ggcgatcggg tcgacgacat cccaccaggc acccgtcacg accatctcct cgcagacggg
     9841 gaggaagtcg aggccgcgga accgggcgaa acctcgccac cgcgcgagct cgaccgccgc
     9901 gtacctctcc tcgcggaacg ccgcgccccg gaagaggccg aggacctcgc ggcggaaagc
     9961 gtccgcgtcg gggagcacga gccgggagaa gagctcccgg caggcggcgc ggagcggcac
    10021 cgcaccgacg ccgtggaacg gcatcgccga cttcatgtac gcccgggcgc cgcgcgcccg
    10081 ctccgggtcg ccgagcgcgg agagccgggc gcggagcgcg aggaggaggc gggtgggacg
    10141 cggcacgaaa ggaattctcc cccggagcct cgcctcggcg ccgtcccgtc cctcccctgc
    10201 gttcccggac gcccgccctt gacatccggc cggccgcgcc ctagtgtatt ggtgttgtga
    10261 tacacagaag cgccaggagc tcctcgtgac cccccgcggc ctccacgtcg acccgcgcga
    10321 cgccgtgccg atctggaagc agatcgagga ggggatgcga cggctcgtcg cctcgggcgc
    10381 gctcgcgccc ggcgcggcgg tcccgtccgt ccgcgacctc gcccgcgacc tccaggtgaa
    10441 ccccgccacg gtcgtccgcg cctaccagcg gctcgtcgag gcgggcctct tcgcggtccg
    10501 ccgcggcgag gggacgttcg tcgccgaggc cccgccgtcc ctcccgaagc cggagcggac
    10561 ccgcgccctc gccgaggggg cgctccgctt cgcctcgacg gccgtcaccg tcggcgcctc
    10621 gcgcgacgag gcggcgcgcg ccctcgaggg cgccttcgcc cgcctcgccc ccccgaagga
    10681 aggagagaag aagtgagcgc aaccgccgcg gcccgcaccg agcggctgac ggtccgctac
    10741 ggccttacga ccgccgtcga cgcggtctcc ctcgacgtcc cgaagggctc cgtcttcgcc
    10801 ctcctcgggc ggaacggcgc cggcaagtcg tcgcttctcc gctgcctcct cggctggcag
    10861 aaggcgacgg gcggccgctg cgagcttttc ggcgaggacc cctggaatcg ccgtgcgcgg
    10921 ctcatggagc gggtcggcgt cgtccccgag gagccggacg ccccgcgcga gatgacgccc
    10981 cgccagctcg gcgctttctg cgcccgactc tacccgtcgt gggacgcgaa agggtacgag
    11041 gcccgccttt cccgcttcgg agtcccgcag gcgaccccgt tcgtccgcct ctcgaaagga
    11101 cagaaggggc tgacgcagct ctccctcgcc ctcgcctcct cccccgacct tctccttctg
    11161 gacgatccga cgctcggcct cgacccggtc gcgcggcacg ccttcttcga cgagatcgtc
    11221 gccgagctgg cggaccgcgg gacgaccgtc ttcctgacga cgcacgacct cgccggcgtc
    11281 gaacgaatcg ccgaccgcgt cgggatcctg aaggacggcc gcctcctcct tctcgaggag
    11341 acggaggccc tgaagtcccg gttccgcacg gtccgctacc ggagcgaggt gaccgaaggg
    11401 cggacggact tcgggaccga gctcgacgag ctcgagccgg tccgcgtgaa ggtccgcggc
    11461 tgggggatcg aggccgtcgt ctcggccttc tccgacgagc gcttcgagcg cttcgccgcg
    11521 aaggacggga tcgtcgacgc cgaggccgcg ccgctctccc tcgaggagct cttcctcgcc
    11581 gtcgccggcg aggggaagga caccatccga tgaaacgctt cgccgccgtc gccctccgcg
    11641 aggtcgcgga gcggcgctcc gtcctcgtcg cggccgccgc ggccgcgatc ctcccgttcc
    11701 tcgtgccgct cctcccgtcc gtcccgggga gcgacgccgc gaccgctcgc gccctcacgg
    11761 cgctcgtcct tgcgtgcgcg ttcggcgccg gcggggcggt cctcgtcggc gcctccgtcg
    11821 tcggccggga gctcgcggag aagcgcctct cgttccactt cgcccggccg ctctccgctc
    11881 ccgtgatctg gggcgggaag gtcgtcggcg ggcttgctct ggtcctcctc gcggagctct
    11941 tcgtctacct gccggcctcg atcgccagcg gccgcttccc ggggctcgtc gaccgcttcg
    12001 acgccccggc gatggcggcg ctcctcctcc tcgccgtccc gctcttcctc atcgcctgga
    12061 tcgcctccgt ggcggttcgc tcccgttcgc cgtggctcgt cgtcgacttc gtcctgcttg
    12121 ctgcgtcggc ggccgtcgtc tggtcgctcg ggaagcgact tctccttcac gggcaggccc
    12181 cgactctcat ggagaccgcg gtcggagccg tcctcctcct cacgatcctc ggcgcagcga
    12241 cgttcgttca ggtctgtgcg ggccgcaccg atgcccgccg cggccacggg gcgcagtcgg
    12301 tcgtcctctg gggagggctg gggatcttcc tcctcgggct cgccgtatgg gtcgacggga
    12361 aggtcgatcc cggctacgag acgttcgtcc acctggcggc ggagccgacg ggatcgagcg
    12421 gggaatggat cctcctgacc ggcacgccga agaccgtcga cgacttccgg agagcgcgct
    12481 acctcgtcga ccttcggacg ggacggagcg tcctcctgcc gctgtccttc ctccacacgg
    12541 tctccgcgga cggctcgcgg gcgacgtgga tcggcccgtt cggacccttc gacaagagcc
    12601 cccgccgcac ggtcgccgac ctgcggtcgg gagactccac ggagtacccg atcggcggtc
    12661 tcggcgacgg cgcgtccggc ccgtcgttcg agctcgccct ctcggccgac ggaagccggc
    12721 tcgcgttcct ctccgccgag ggagtctgcg acgtcaccga tctcgcgacg ggccggctcc
    12781 tcgcctccgc gagggtcccc ggcatcgagc gctggttccg ccaggtgtcc ttcgattccc
    12841 gggacgtcgt ccgaatcgag ccgaggactc gaatggccga ggagatgggc cacgcgcacg
    12901 agctcgcgcc gcttccgccc gctccggccc tcttcctcct cgacgtcccg tcccggacgc
    12961 tgaagccggt cgggacggcc ctcgcacccg ctccgttctt ccagagagtc gtcggtccga
    13021 cgacgacgcc gcgagcattc gtccgccgcg ccgacgaccg ccggcgactg cacctcgtcg
    13081 acctcatgaa cggcgccgcc ctcgcatcct ttggaagcgc cgagacgaag ggggggcacg
    13141 tgcagggggg ctcgctggcc gacggacgcc tcttcgtcaa ggtcgacgac cccggtaccg
    13201 cctcgcgaat cgtccttctc tctcccgacg ggacgaccct tcgcgaagtg aacctcccgg
    13261 acgacgcggg cggatgggac gtcggcctcg aggcagcgcc cggcgtcctc ccgatcgcga
    13321 ggcccgagtg gttgcgcaag gaggagccgc acagcctcct cgtggacctg aataccggcc
    13381 agacccgatc gctcggacgg ctccgtctcg gcgtccgttt cggcctgtcc tccgtcgtct
    13441 ctccggtcgt ccccccaccc gtcggctcac ccgcgacgcg gctgttccgc gatgccgaca
    13501 ccggccgcct cgtcctcctc gatccgcaga cccaggagcg aaaaccgctg acgcgcggcc
    13561 ggccctccgg caagtaagat cgaccccgtg accacccccg cacgccgcga agcccgccgc
    13621 gtcctcgaga tcgaggccgc ggcgatccgc tccctcgccg actctctcga cggcggcttc
    13681 gacacggccg tggccctcct gcacgggacg acgggccgcg tcgtcgtcac cggaatcggg
    13741 aagagcggga tcgtgggcca gaagatcgcc gcgacgctcg cctcgaccgg gacgcccgcg
    13801 ttcttcctcc accccgcgga ggcgatccac ggcgacctcg ggatgatcct cgcgggggac
    13861 gtcgtcctgg ccctctcgca ctcgggcgag acggaggagg tcgtggcgct cctgccgcac
    13921 gtgaggcggc gcggcgcgaa gctcgtcgcc ctcaccgggc gccgcgcctc gacgctcggc
    13981 aaggaggccg acgccgtcgt cgaggcggcg atccgcgagg aggcctgccc gctgaacctc
    14041 gcgccgacgg cctcgaccgc ggcccagctc gcgctcggcg atgccctcgc gatggcgctg
    14101 tcggtcgaga aggggttccg gccggaggac ttcgccgcgc tccatcccgg cggcaagctc
    14161 ggcaagcgct tcctccgggt ctccgacctg atgcacgccg gaagcgacgt gccgctcgtc
    14221 ccgctcgacg ccccgatgaa ggacgtcgtc tacgagatgt cgaagaagcg gctcgggatc
    14281 accggcgtcg tcgacggcga cgggcgcctc gccggcgtcg tctccgacgg agacctccgg
    14341 cgcctcctcg agcgtgaggg ggagaaggcg tggagcgccg acgcggcctt cgccatgaac
    14401 cgaacgccga agacgatcgc ggcgggggcg ttcgccacgg aggcgctccg gctgatggag
    14461 gagaagaaga tcacctcgct cttcatcgtc gacggcgacg ggcgccccgg cgggatcgtc
    14521 cacctccacg acctctgggg cgtcgagtcg atttgagccc ggggccggct ggcggataat
    14581 cgctcgtcat gcccgcagca ccgatcgtcc ccgacgacct ccggacgaag ctcctccggg
    14641 tccgcgccct cgtcctcgac gtggacggag tgctcaccga cgggacactc actttcgacg
    14701 aagacggccg ggagctgaag tcgttcgacg tgaaggacgg gctcggcctc gtcctcctcc
    14761 gcgacctggg gtaccggatc ggcctcatct cgagccgcgt ctcgaaggtc gtcgcccttc
    14821 gctgccgcga cctgaagatc cccgaggagt gcgtgatgca gggggagctc gacaaggcct
    14881 cggctttcga ccggctcctc ggaaagtggg gattcgaagc gttcgaggcg gtggccgtcg
    14941 gggacgacct ccccgacctc gggatgatgc ggcgcgccgg gatcggcgcc tgcccgtccg
    15001 acgccgtcac gatcgtgaag gacgccgccg ccctccacct gacgaaaccg ggagggggcg
    15061 gcgccgtgcg cgagctctgc gacctgatcc tggagatccg gcgtggcgcg agggccgaca
    15121 agccgcagga ggtcaagccg ccccaggcgt cctcggacgg ctccgtcgtc ccgttcccgg
    15181 gctcgcgcgg gtgatcctga atcggggagg ctcgccacga gcctccccgc ggcgccttcg
    15241 gcgcctcccc tccatcgggc gccggagacg aatcggggag gctcgccacg agcctccccg
    15301 cgatcagacc gccggctcgg tcttcttccg cttcccgacg atccaggcga ggaagatccc
    15361 caggaggtag agcgcgatca tcgggagggc gaaggcgcac tgcgtcggga tgtcgggcgt
    15421 gggcgtgatg agcgccgcga tcaggaagat gacgaggacc gcgtagcgga acttctggag
    15481 aagccacttc tccgtcacga cgccgatccg cacgaagaag acgatgacga tcggggtctg
    15541 gaagacgagc cccatcccga ggaggatctt cgagaggatc gagaggtagt cgtcgatctt
    15601 caggaccggg gtgaagtcgg cgcccatcgt cacgaggaac tgcgccacca tcgggaacgc
    15661 gaccgcgtac ccgaaccacc ctccgccgca gaaggagagc gccgcgaaga agacgaacgg
    15721 gacgacccac ctcttctcgt ggcggtagag accgggcgcg acgaaaagcc acacctgata
    15781 caggatcacc gggctcgcga ggaagatccc cgcgatcagg gcgatgttca ggtagagcat
    15841 gaagggctcg gtcaggttcg tgtaggcgag cttcgtcccg gccggcagga cctccaggat
    15901 cggcttctgc gcgatccgga acaggtcctg ggccttccac cacgagagga agaagccggc
    15961 ggccacggcg atcaccgacc agagaaggcg cttgcggagc tcctcgaggt gctcgaggaa
    16021 cgtcatccgc gtcaggtcgt cggggggggc atccgtcacg cgggccggat cgggtcggag
    16081 cgctcttcgc cggcgctcgc gacggtcccc tcggcgggga cggcctgcgg ggcggcgtcc
    16141 ctcttcggcg cctcgtcccg ggccgccttc tccacctcct cctcgatcga acgcttgagg
    16201 tcgttcgagg ccctcttgaa ctcgccgaga cccttcccga gctgctttcc gagctcgggg
    16261 agctttttcg gtccgaggat caggagggcc agagcgaaga tgaagatcag ctccggcatt
    16321 ccgaggttgc ccatggccgc ggaatcgtcc gctcagcccc gcccggagtc aaggacgacg
    16381 tgccggcctt gtcgaagctc catggaaatg tcacggtgcc agctcgatag aaatgtccgg
    16441 g
//
LOCUS       DAOONL010000077        16301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3765_length_16301_cov_20.108088, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000077 DAOONL010000000
VERSION     DAOONL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16301
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3765_length_16301_cov_20.108088"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..721)
                     /locus_tag="PLP50_11650"
     CDS             complement(245..721)
                     /locus_tag="PLP50_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52245.1"
                     /translation="MMRSSPLAVLLLFTAANAPSQTPAEPSAVRCDAVLTAEEAVVGD
                     GHQGPAVDEPRPGFTSCDWQGDGSNFGFTFASLKALAADERSADDEFEFDVQALESES
                     TKREVLPGIGVKAAILPAGEDAFAVIVQRTDGVARMITYKVEREKALALARALATP"
     gene            complement(1588..2163)
                     /locus_tag="PLP50_11655"
     CDS             complement(1588..2163)
                     /locus_tag="PLP50_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52246.1"
                     /translation="MDRKEFFRRTLQLGLGCCAGAASEGFALAGEPTAPASLPSRELE
                     AARHDADFVRGWTSDLLDAMDQELPLETRQKLVAACGRGCYRRHAFKPAIAEKGRGDV
                     ARLVAAYAEMFECWRDGESVHVRYGETSKHCYCPVVRDRPAKPSDLHCECTRATHQSI
                     FEAALGRPVPVEVVETLRRGGKTCHFVARLA"
     gene            2303..3187
                     /locus_tag="PLP50_11660"
     CDS             2303..3187
                     /locus_tag="PLP50_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPA52247.1"
                     /translation="MAVAFRSAPRFSGALLLPVLALLAACASRPVEPARSRARPDLER
                     ARSRFSQHGVTGASVVLTPSVVSNPESATQAIVIESGDDAGRRTIPASTLEFPNTLIG
                     LEAGAVKPDEVFRWDGKPREFGAWERDLALVEAFRVSCLPCYQEAARRVGIGLMRRLL
                     RERGYGNSEVGDSVDSFWIAGPLKFSVWEEAEFVRRVSLGDLPFGVDHLETLWASMKL
                     DEASAYRLFAKTDWAGMTNRVDPGVGWFVGWVETPEGPRSFATRVVIPQEGPKTDLTF
                     RKRLTLELLKDVGAIRLR"
     gene            complement(3555..4265)
                     /locus_tag="PLP50_11665"
     CDS             complement(3555..4265)
                     /locus_tag="PLP50_11665"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPA52248.1"
                     /translation="MGKTTPLAVTTSQRRKLEALVAQPSASAGHVRRARVVLMAADGI
                     PGVEIATRLSLSVPQVSRIRKRFEVGKVEGLADRPKPGRGNSVPEHVVCEVVTTVMSP
                     PPAGHSHWSTRLLAGAVGLGKTAVHEILKANDLKPHLQRTFKVSKDPAFAEKVRDVVG
                     LCLNPPENAVVISLDEKAQVQALDRTQPLLPLKPGRSNEERTTTPGTASWICTLRPRS
                     LRAKSSAVARTRTPARTL"
     gene            4889..9238
                     /locus_tag="PLP50_11670"
     CDS             4889..9238
                     /locus_tag="PLP50_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HPA52249.1"
                     /translation="MLLAASPELWFRKALFSWIGKRSGLCDGVDLPWFDCEGQVTFDS
                     RVFPRVLRSFEGRELVFEVTVERLDREEFQGLASTAGQIHDFTYLRFTPGGPAVAAGK
                     AVKAEIYKPDGLRRRSGAGNRLSPVHRLIVTPAGARVGPDGGRKRRRWGRGPVRRNPV
                     GEERADAAYDRPMEQVVRWVLLAVLCAATGTAQAQDTTFLTFNSEPGDHVGGGLRFTL
                     TSSDGLIAAYPIDGGVQLYFDGSTFWHLYFVPPKGSTLTPGVYDDVRPWPLQSATAGG
                     LYVFGDGRGCSMITGRFQVLEAEFGPGGEVQKLAVDYEQHCQGAEPALFGSVRYKSGV
                     PVGPRLGVGAITRYEGDDGWWNLGLVVSLSGPADTAVSVDYETLDGTATAGADYAAVS
                     GTATIPAGETDAVVPVPVLGDATVEADETFTLSLSNAVGAPIAFAQGVATLRNDDPYK
                     TVLRLDSEPGDFVGQGQSVTVTPIDGDFNLEKTDDGGVHVRFFGTTSFLWDLNFAPPA
                     GAALAPGGYEGATRWPFQSPTAPGLEVSGGGRACAWLAGRFVVHEAVFGPQGEVLRLA
                     VDFEQHCEGDDAALYGSIRYESNVPIGPRLFVGPVRILEGDGGSASLGYVISLSEPAA
                     AAVTVDYQTVDGSATAGSDYVGVSGTATFPAGETSVPVSVPVIGDLVEEGDETFALSL
                     SNAAGASIAFGEGQGTILNDDPYKTFLRFDSQPGDLLGQGLQFTFTEVDGSITAAPLE
                     GGIEVVFYRGSWWKLRFVPPLGETLAVGAYDGATGWPFQSPTTPGLGVTGEGRGCNVS
                     AGRFTVLEAEYGPAGEVLRFAVDYEHHCNGNPAALFGSVRYDSGVPVGPRLAVGAVDR
                     YEGDDSSTSMGFVISLSAPSGSLVTVPYQTVDGTATAGVDYVPVAGTATFPAGQTTVP
                     VPVQVLGDTAQEPDETFTLSLGDGSGAPVAFAQGLGTILNDDPFKTLLYFNSEPGDFI
                     GGGLRFTMTPADGAFALSRFEGGVYVAFTGPEFWQLLFAPPAGTTLAPGVYPAAVRAT
                     TTRPPGSPGLEVMGAGRACQALTGRFAVLEAEYGADGEVLRLAVDYEQHCLGGDPALF
                     GSVRYRSSVPVTRRLIAADASVAEGDSGTRSLGFPVWLSEPGGSPVTVSFATADGTAR
                     RGVDYAPRSGSTTLAAGQTDVVIPVTVYGDTLPEADETFSLTFPGVVGAAAVFPPEAV
                     GTILTDDMLPQLNVGDVSVVEEGPGSSPMASFVVSLSAPSDQPVSVAYLTQSGTATAG
                     ADYVHTAGTLEVAPGAASGTIAVPIVPDRLAEATETFSLLLLSPANATIGRGQALGTI
                     LDDDGDGPLSFYTLLPCRLLDTREAGPALDVGVERVVSAVGACGIPPEARAVVLNVTS
                     VEATGAGDFQAYPAGTAAPDTSVVSFRAGRTRAGIAVVRLGADGGLALTCRMPGSSNG
                     SAHAVVDVSGYFR"
     gene            complement(9330..12344)
                     /locus_tag="PLP50_11675"
     CDS             complement(9330..12344)
                     /locus_tag="PLP50_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52250.1"
                     /translation="MNDTTLRFALARRAALGLFPLVLAVAAAPRLSAGENAWSPIGPA
                     GTEVLALAVSPVSSSTIVAGTSTGYSVTRNGGASWSAVSTGYSTAAIAFDPANASIVY
                     AATGGALARSADGGTTYTPVLLEATTCLAIDPSSPLVVYAGTTDHVYKSIDRGLTWNV
                     VYTGVGHMFVNAIAVDPSSPQTVWAASTFGGLMKSTNGGTSWSPVSGGVPILVSAIAV
                     HPSASSTVLLLGMDGVYRTTDGGTAWSRVTNLWGSDGELVADRASPGTFYASGSDGVT
                     FSADGGATWNHLDSGLTNLDVLALALDPADHTRLYAGTNGNGVFSMTVGSGGAPTLRV
                     TSPNGGEVWEAGTTHPITWTSSGSIANVKIEYSTTSPWDGQLVDPQTVVASTPNTGTF
                     PWVVAQSMSPVCHVRVSDASGTTSDMSDAAFRIVTCGFTGLNPPFSQAFGAAGGRGTI
                     QVTPSSGCPWTATSEVPWVVVTSGWSGTGSGSLTYSVAPNLDSSPRTGTLRIGTASFE
                     VEQSGGTTTTEGVVFVPVVLDVFGVAPSHYTSELTLTNRSARDATLRLAYTGAATLGG
                     GAGSATVTLPAGRQVVEPDAIAYLGRLGVGVPATAPRGGTLYVAVSGVTSLSEVAATV
                     RTTTAVANGQAGLAYGGTPAWRALTGAAFVCGLRENATDRSNLALQNAGAPADGDVTL
                     RVTVCSGDSSARVVRPDETLPPGGFLQVSGILGLHGMTNGYAKVERVGGTAPWYAYGV
                     VNDQKNSDGSFVPPQPATTAPVSSLTVPVVIEASVYTSELVATNWSSRARTLTLSFVS
                     DQVDRPDHTASVSLPLGAGAQVILPNVFAYFRDHAAPGIGPAGSGYVGALFVTSSDGD
                     LSGIVVGARTSSPGGGGRYGLFYPATPSGGASTDSAWLYGLQQNAANRTNLAIVNTGE
                     AGGDDDVFAVDVYDGATGHLVHTATGLTVKAKRWWQQNNALSTWAPGVADGYVRVRRT
                     SGTNPFIAYAVINDGGVPQQRSDDGAFLPASD"
     gene            12805..13317
                     /locus_tag="PLP50_11680"
                     /pseudo
     CDS             12805..13317
                     /locus_tag="PLP50_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
     gene            complement(15322..15717)
                     /locus_tag="PLP50_11685"
     CDS             complement(15322..15717)
                     /locus_tag="PLP50_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA52251.1"
                     /translation="MIVLDASALVELLLGTKSGRAIAARIADPSLGLHVPHLADVEVT
                     QALRRFVRTGELDAAAGTSTLEDLRALDPERHPHEPLLGRVWALRDGLTAYDAVYVAL
                     AEALDTVLLTCDARLAHAPGLSRRVELIR"
     gene            complement(16020..>16301)
                     /locus_tag="PLP50_11690"
     CDS             complement(16020..>16301)
                     /locus_tag="PLP50_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPA52252.1"
                     /translation="APGYPACPDHTEKRTLFSLLDATANAGITLTESYAMLPTASVSG
                     LYLAHPEAHYFAVGRLGRDQVADYARRKGMPLHEAERWLAPNLGYEAEG"
BASE COUNT         2291 a         5565 c         6009 g         2436 t
ORIGIN      
        1 gaaagagacg ccgtggggga gcgcgacgcc gatcaccacg gcgcggcagt tcgacgcggc
       61 cgtggcggcg ctcttcgaga ggctcgcccg ccagaggatc gtctgggtcg aggggaccca
      121 cctgccgcag gacgttcagg cgggtgccct cgcgggcgcg gtccggatgg cgcgctgagg
      181 cccgggtcgg gccgcgcgac gcggaccgcg gaacgggggc gaaaaggccc tccggccccg
      241 gccctcaggg cgtcgcgagg gcccgcgcga gagcgagcgc cttctcgcgc tcgaccttgt
      301 acgtgatcat ccgcgcgacg ccgtcggtgc gctggacgat gaccgcgaag gcgtcctcgc
      361 cggcggggag tatggcggcc ttcacgccga taccgggcag cacctcgcgc ttcgtcgact
      421 cgctctcgag cgcctgcacg tcgaactcga actcgtcgtc ggcgctcctc tcgtcggcgg
      481 cgagcgcctt caggctggcg aacgtgaagc cgaagttgct gccgtcgccc tgccagtcgc
      541 agctcgtgaa cccgggccgc ggctcatcga ccgcggggcc ctgatggccg tcgcccacga
      601 cggcttcctc ggcggtcagc accgcgtcgc accgcaccgc cgaaggctcc gcgggcgtct
      661 gggacggggc attggcggcg gtgaagagaa gcaggacggc gagcgggctc gatcgcatca
      721 ttcgacctcc tccgggcggg tgtcggacgt tcggacccgg gcattttcgt tcacggggtc
      781 ggcggccgcg tcaagtcgag agacgagcga ggggcctccg gcactctcga ggccggcgac
      841 gtccgcccgc gaagcaccgc atgggctgac ccgtcggagc ggctcgtcgg cgcctttgtg
      901 cgggccagac ggggagacgc cgaggggatc gccgccgacc cgcgggacgg cgcgctctac
      961 gtcgggagca tcgcgaagcg gaaggtcgtc cgcgtcgcgg tgtccggagc cgcgagcgac
     1021 ttcggcgcag gtgaagcgtc cggtctcggc gaggtcctcg ggctgaaggt cgaacccgcc
     1081 cgctcggccc tccaggcctt cgacctcacc tcgggcgcgc cgcgcgtctc ggtcgcgacc
     1141 gaggagccga agcacctcct gaacgacctc gtgatcgtcg acggcgtcct cctcgtctgc
     1201 gacgcgctcg gccgctgggc tctcgacggc gatggagccg cgccacggcc cctcgtcgcg
     1261 cctggagggt ccccgttcgg aggaatcgac gggctttcgg cccgcggacg gacgctcgtc
     1321 ggcgtccaga gctcgctcgg agcgccgcgc atcgtctgtt tcgacgtcgc gccctcggcc
     1381 cgcgccgttc tccgggccga ggtgctcgaa gcggggaacg cgctctggcg cataccgacg
     1441 acccgcgcag ctctaccgcc tgagaatccc gtcctgatcc cggcccatcc gtcttcgacg
     1501 agcgcgccgc cgggcgcgca cggtccctcg tcgccgcaat ccgagtcgaa ccgaggagtc
     1561 gtcgaccggt cgcgagacgg ggatgactca ggcgaggcgg gcgacgaagt ggcaggtctt
     1621 cccaccgcgg cggagcgtct cgacgacctc gacggggacg ggcctgccga gcgcggcctc
     1681 gaagatggac tggtgcgtcg cgcgcgtgca ctcgcagtgc aggtcggacg gcttcgccgg
     1741 ccggtcgcgc acgacggggc agtagcaatg cttcgaggtc tcgccgtacc gcacgtggac
     1801 gctctcgccg tcgcgccagc actcgaacat ctccgcatag gcggcgacga gcctcgcgac
     1861 gtcgccgcgc cccttctcgg cgatcgcggg cttgaacgca tgccgccggt agcagccgcg
     1921 gccgcaggcc gcaacgagct tctgccgggt ctcgagcggg agctcctggt ccatcgcgtc
     1981 caggaggtcg ctcgtccagc ctcggacgaa atcggcgtcg tgccgggccg cctcgagctc
     2041 cctggatgga agggaggccg gcgcggtggg ctcgccggcg agcgcgaaac cctcggatgc
     2101 ggcgcccgcg cagcagccga ggccgagctg gagcgtccgc ctgaagaatt ccttccggtc
     2161 caccgacgcc tccggccgga cccgtgccgg gaccggcccg tttcgcggga ctgaggcttt
     2221 gtacgcggcc gccacccggc atgttgcgcc gcagcgccgg cggaggggcc tcctcgtaga
     2281 ctcgcgaggt cggaggagca tcgtggccgt cgcgtttcgg tcggcacctc gcttctctgg
     2341 agcgcttctc ctgcccgtcc tggccctcct cgcggcctgc gcctcacggc ccgtcgagcc
     2401 ggctcggtcc cgcgcgcggc ccgacctcga gcgggcgagg agccggttct cgcagcacgg
     2461 agtgacgggc gcgtccgtgg tcctgacccc gagcgtcgtc tccaatcccg agtctgcgac
     2521 gcaggcgatc gtgatcgaga gcggcgacga tgccgggaga cggacgatcc cggcctcgac
     2581 gttagagttc ccgaacaccc tgatcgggct cgaggcggga gcggtgaagc ccgacgaggt
     2641 cttccgctgg gacggcaagc cgagggagtt cggggcctgg gaacgcgacc tcgccctcgt
     2701 cgaggcgttt cgagtctcct gtcttccgtg ctaccaggag gcggcgcgcc gggtcgggat
     2761 cgggctgatg cgaaggctcc tccgcgagcg cggatacggc aactccgagg tcggcgactc
     2821 cgtcgactcc ttctggatcg ccggaccgct gaagttctcg gtgtgggagg aagccgagtt
     2881 cgtgcgtcgc gtctccctcg gggaccttcc tttcggcgta gaccacctcg agaccctgtg
     2941 ggcttcgatg aagctggacg aggcttcggc ctatcggctc ttcgccaaga cggactgggc
     3001 gggcatgacg aatcgggtcg atcccggcgt gggctggttc gtcggatggg tcgagacgcc
     3061 cgagggacct cgctccttcg ccacccgggt cgtgatcccg caggaggggc cgaagaccga
     3121 cctgaccttc cggaagaggc tcaccctcga gctcctgaag gacgtcggcg ccatccgtct
     3181 acgctgatcc tcgtgctccg cccgcgagat ccgcgcaagc atgcgctgag gatcgcggac
     3241 gatcgtgcgt gaggactctg tccacacgaa aggtgtcggg tcctcgttcc aacgggcgag
     3301 gaaggcctcg atgtgccgcc gaagcgccgc cgtggaggcg aagctggctc gcctcaagga
     3361 ccgcctcgtc aggatgctga agaagccttc gacctggttc agccacgacg cgctcgtcgg
     3421 agtgaagtga aagaccacgc gaggatgctc ggcgagccac ctctgcgcgt ccggcgtctt
     3481 gtgcgtggag gagttgtcga ggatgacatg gagctcgcct cgacggaagg tacgctccac
     3541 ggtcctcaag aagctcagag agtcctggcc ggtgtgcgag tcctggcaac ggccgacgac
     3601 tttgcccgta gcgatctcgg gcgcagcgta cagatccacg acgccgttcc aggtgtagtc
     3661 gtgcgttctt cgttcgacct gcccggcttc agtggcagca acggctgcgt ccgatcgagg
     3721 gcttggacct gcgccttctc gtcgaggctg atgacgacgg cgttctcggg cgggttgaga
     3781 cagagcccaa cgacgtcgcg caccttctcg gcgaacgccg gatccttgct gaccttgaag
     3841 gtgcgctgaa ggtgaggttt gagatcgttg gccttcagga tctcgtgaac ggcggtcttc
     3901 cccagaccga cagcaccggc cagaagccga gtggaccagt gagagtggcc agcgggcgga
     3961 gggctcatca cggtcgtcac gacctcgcac acgacatgct cgggaacgct gttcccgcgt
     4021 ccgggcttgg gacggtcggc gaggccttcc actttcccga cctcaaaccg cttccggatc
     4081 cggctcacct gcggtaccga cagcgacagc cgcgtcgcga tctccactcc ggggattccg
     4141 tccgcggcca tcaacaccac tcgggctcgc cgaacgtgtc ccgccgaagc cgacggttgc
     4201 gccacgagag cttcaagctt gcgcctctgc gaggttgtga ccgcgagagg ggtggtcttt
     4261 cccatggctc ttcatgcgag gcgaccccat tcatggttgc acgtgtttcg cgggcgccac
     4321 actaggcgcg gaggaacggc gaccattcga cgtacgggac gaggtcggcg agggccgcct
     4381 cctcgacgcg gaccctggtg agaacgggcg cggggaggtc ggccgcggtc caggcgaagg
     4441 cccggcggca ggcgcgggcc gaggagagga ggaggcgggg tctgctctgc cggtacgcat
     4501 ggtcgcggtg cagctcctcc tgcagcgcgg agtgcctcgc gtcgaacgcg gggcggtgat
     4561 cgtcggagag gaggcctctg gcgacgccga cggcccggct cgcgtcggtg atctggacga
     4621 cgggctgcgg aaaggccggg ggcgatcctg atgggggtgt gcgccgggct cgtcgccgtg
     4681 cgattcggcg cgagggtcga ccccatgccc gaggagctga cgaggaagct cgaggaggtc
     4741 ctccggaaga atggtgggca caggaggacc cggtgcaagc ccgcgaagat ccggccagac
     4801 gacggacgcg cacgagaggc ccggaaagac ggtctccgag cctctgacag cttcgccatg
     4861 gttgacgggg tcaagcgagg gcgagtacgt gctgctcgcc gcttcaccgg agctgtggtt
     4921 caggaaggcc ctgttctcgt ggatcggcaa gcgttccgga ctctgcgacg gagtcgacct
     4981 gccctggttc gattgcgaag gccaggtcac gttcgacagc agggtctttc ccagggtgct
     5041 gcgctccttc gagggaaggg agctcgtctt cgaggtgacg gtcgagcggc tcgaccggga
     5101 agagttccag ggcctggcca gcaccgccgg gcagatccac gacttcacgt acctacggtt
     5161 cacgccgggc ggtccggcgg tggcggccgg gaaggccgtg aaggcggaga tctacaagcc
     5221 ggacggcttg cggaggcgtt ccggggccgg caaccgtcta tctcccgtac accgtctgat
     5281 cgtgaccccc gccggcgcgc gcgtcgggcc tgacggcggg cggaagcgac ggaggtgggg
     5341 gcgggggccg gtgagacgga atcctgtcgg agaggaacgt gccgacgcgg cctatgatcg
     5401 tccgatggag caggtcgtgc ggtgggtcct cctcgcggtc ctgtgtgcgg cgaccggaac
     5461 ggcgcaggcc caggacacga cgttcctcac cttcaacagc gagcccggcg accacgtcgg
     5521 cggcggactc cggttcacgt tgacctcgtc ggacggcctc atcgccgcct acccgatcga
     5581 cgggggagtc cagctttact tcgacggctc gaccttctgg catctctact tcgtcccccc
     5641 caagggatcg accctgacgc ccggcgtcta cgacgacgtg aggccctggc cgcttcagtc
     5701 cgcgacggcg ggcgggctct acgtcttcgg agacggccgg ggctgcagca tgatcaccgg
     5761 ccggttccag gtcctcgaag ccgagttcgg cccgggaggc gaggtccaga agctcgcggt
     5821 cgactacgag cagcactgcc agggagccga gccggcgctc ttcggctcgg tgcgttacaa
     5881 gtccggcgtg cccgtcggcc cgcgtctcgg ggtcggcgcg atcacccgat acgagggaga
     5941 cgacggctgg tggaacctcg gcctcgtcgt ctccctctcc ggaccggccg acacggcggt
     6001 ctcggtcgac tacgagacgc tggacggcac ggccacggcc ggagccgact acgccgcggt
     6061 ctcggggacg gcgacgatac cggcgggcga gacggacgcg gtcgtgcccg tgccggtgct
     6121 cggcgacgcg acggtggagg ccgacgagac gttcacgctc tcgctgagca acgccgtcgg
     6181 ggcgccgatc gccttcgccc agggcgtggc cacgctgagg aacgacgacc cctacaagac
     6241 cgtcctgcgc ctcgacagcg agccgggcga cttcgtcggg caggggcaga gcgtcaccgt
     6301 gacgccgatc gacggcgact tcaacctcga gaagaccgac gacggcgggg tccacgtccg
     6361 cttcttcggg acgacctcgt tcctctggga cctcaatttc gccccccccg cgggtgcggc
     6421 cctcgcgcct ggaggctacg agggggcgac gcgctggccg ttccagtcgc cgaccgcgcc
     6481 cggcctggag gtaagtgggg gcggacgtgc ctgcgcctgg ctcgccggcc ggttcgtcgt
     6541 tcacgaggcg gtattcggcc ctcagggcga ggtcctcagg ctcgcggtgg acttcgagca
     6601 gcactgcgag ggggacgatg cggcgctcta cggatcgatc cgatacgaat cgaacgtccc
     6661 tatcggcccc cgcctcttcg tcggaccggt ccggatcctc gagggggacg gcggctcggc
     6721 gagcctggga tacgtgatct ccctttcgga gcccgcggcg gccgcggtca cggtggacta
     6781 ccagaccgtg gacgggtcgg ccacggccgg cagcgattac gtcggcgtct cgggcacggc
     6841 caccttcccc gcgggtgaga cgagcgtgcc ggtgtcggtg ccggtcatcg gcgacctcgt
     6901 ggaggaaggc gacgagacct tcgctctctc gctgagcaac gccgcgggcg cgtcgatcgc
     6961 cttcggcgag ggccagggga cgatcctgaa cgacgatccc tacaagacgt tcctccgctt
     7021 cgacagccag cccggcgacc tgctcggcca gggattgcag ttcaccttca ccgaggtgga
     7081 cgggagcatc accgcggccc cgttggaagg cggaatcgag gtcgtcttct accgcggctc
     7141 gtggtggaag ctccggttcg tcccgccgct gggggagacc ctggcggtgg gcgcctacga
     7201 cggggcgacg ggctggcctt tccagtcgcc gacgacgccc gggctgggcg tcacgggaga
     7261 agggcggggc tgcaacgtct ccgccggccg cttcacggtc ctcgaggccg agtacggccc
     7321 cgccggcgag gtattgaggt tcgcggtcga ctacgagcat cactgcaacg ggaatcccgc
     7381 ggcgctgttc ggttcggtgc gctacgactc cggcgtgccc gtcggtccgc gcctcgccgt
     7441 cggcgccgtc gaccgctacg aaggggacga cagctcgacg agcatgggct tcgtgatctc
     7501 gctgtcggcg ccttcgggat ccctggtcac cgtgccgtac cagaccgtgg acggcacggc
     7561 gacggccggc gtcgactacg tgccggtggc gggcacggcg accttccccg ccggccagac
     7621 gacggtgccg gttccggtgc aggtcctcgg cgacacggcc caggagcccg acgagacgtt
     7681 cacgctctcg ctgggcgacg gctcgggggc gccggtcgcc ttcgcccagg gcctgggcac
     7741 gatcctgaac gacgacccct tcaagacgct cctctacttc aacagcgagc ccggcgactt
     7801 catcggcggg gggctccggt tcaccatgac gccggcggac ggagccttcg ccctgagccg
     7861 cttcgagggc ggcgtctacg tcgcctttac ggggccggag ttctggcagc tgctcttcgc
     7921 cccgccggcg gggacgacgc tggcgcccgg cgtctacccc gcagcggtgc gcgccaccac
     7981 cacccgcccc cccggttcgc ccggcctgga ggtcatgggg gccggccggg cctgccaagc
     8041 gctcacgggc cgcttcgcgg tcctggaggc cgagtacggc gccgacgggg aagtcctgag
     8101 gctcgcggtg gactacgagc agcactgtct cggaggcgac ccagcgctct tcggctccgt
     8161 gcgttatcgc tcgagcgtgc ccgtgacccg caggctcatc gccgcagatg cgtccgtcgc
     8221 cgaaggcgac agcggaacga ggagtctcgg attcccggtc tggctctcgg agccgggcgg
     8281 ctctcccgtc accgtcagct tcgccacggc ggacgggacg gcgcgccggg gagtcgacta
     8341 cgcgccgcgc tccggctcga ccaccctggc cgcggggcag accgacgtcg tcatcccggt
     8401 caccgtctac ggcgacactc tccccgaggc cgacgagacc ttctcgctga ccttcccggg
     8461 ggtcgtgggc gccgccgccg tgttccctcc ggaggccgtc ggcacgatcc tgacggacga
     8521 catgcttccc cagctgaacg tcggcgacgt cagcgtggtc gaggaagggc ccggctcgtc
     8581 tcctatggcc tccttcgtcg tgagcctgag cgcaccgagc gaccagccgg tttccgtggc
     8641 gtacctgacc cagtccggca ccgccacggc gggtgcggac tacgtccaca cggcgggcac
     8701 gctcgaggtc gcgcccggcg cggcctccgg gacgatcgcc gtgccgatcg tccccgatcg
     8761 gctggccgag gccaccgaga ccttctccct gctcctcctg tccccggcga acgcgacgat
     8821 cggccgtggc caggccctgg gcacgatcct ggacgacgac ggggacgggc ccctctcctt
     8881 ctacacgctc ctgccctgcc ggctcctcga cacgcgggag gccgggccgg ccctggacgt
     8941 cggcgtcgag cgggtcgtat cggccgtcgg ggcctgtggg atcccgcccg aggccagggc
     9001 cgtcgtcctg aacgtgacgt cggtcgaggc gacgggcgcc ggcgacttcc aggcctaccc
     9061 ggcgggcacc gcggctccgg acacgagcgt ggtcagcttc cgggcggggc ggacgcgggc
     9121 cggcatcgcc gtcgtgcggc tcggcgcgga cggaggcctc gcgttgacct gccgcatgcc
     9181 cggcagctcg aacgggtctg cccacgcggt ggtcgacgtc tccggctact tccgataggc
     9241 ggaacgagcg cggagagacg ggcgccgggg agggcggggc gcgccgcggc gggagggccg
     9301 ggacgcgccc cgggaaggcc gggaggacgt cagtccgacg cggggaggaa cgctccgtcg
     9361 tcgcttcgct gctgcgggac gccgccgtcg ttgatgacgg cgtaggcgat gaagggattg
     9421 gtgccgctcg tccggcggac gcgcacgtag ccgtcggcga ctccgggcgc ccacgtcgag
     9481 agggcgttgt tctgctgcca ccagcgcttc gccttcacgg tgagcccggt cgccgtgtgg
     9541 acgaggtgcc cggtggcgcc gtcgtagacg tcgacggcga agacgtcgtc gtcgccgccg
     9601 gcctcgccgg tgttcacgat cgcgaggttc gtccggttcg ccgcgttctg ctggaggccg
     9661 tacagccagg ccgagtctgt cgaggccccg ccggacggcg tcgccgggta gaagaggccg
     9721 tacctcccgc ccccgcccgg agacgacgtg cgggcgccga cgacgattcc cgagaggtcc
     9781 ccgtcggacg acgtgacgaa gagggccccg acgtatccgc tccccgccgg cccgatcccg
     9841 ggggcggcgt gatcgcggaa gtacgcgaag acgttcggga ggatcacctg cgccccggcc
     9901 ccgagcggga gggagacgga cgccgtgtga tcggggcggt cgacctggtc ggagacgaag
     9961 gagagcgtca gcgtccgcgc gcgggacgac cagttcgtcg cgacgagctc gctcgtgtag
    10021 acgctcgcct cgatgacgac cgggacggtg agagaggaga cgggggccgt cgtcgccggc
    10081 tgcggcggca cgaaggaccc gtcggagttc ttctggtcgt tcacgacgcc gtaggcgtac
    10141 cagggagcgg tgccgccgac gcgctcgacc ttcgcgtagc cgttcgtcat cccgtgcagc
    10201 ccgaggatgc cgctgacctg caggaagccg cccggcggga gggtctcgtc ggggcggacg
    10261 acacgggcgg acgaatcgcc ggagcagacc gtcacgcgga gcgtgacgtc cccgtccgcc
    10321 ggggcgccgg cgttctggag ggccaggttc gagcggtccg tcgcgttctc gcggaggccg
    10381 cagacgaagg cggcccccgt gagcgctctc cacgcgggcg tcccgccgta ggcgaggccg
    10441 gcctggccgt tggcgacggc ggtcgtcgtc cggacggtag cggcgacctc ggagagggac
    10501 gtgaccccgc tgacggcgac gtagagggtc ccgccgcgcg gcgccgtggc cgggaccccg
    10561 acgccgagcc ggccgaggta cgcgatcgcg tcgggctcga cgacttgccg gcccgcgggg
    10621 agcgtgaccg tcgcggagcc ggcgccgccg ccgagggtcg ccgcacccgt gtaggcgagc
    10681 cggagggtcg cgtcgcgggc cgagcggttc gtcagcgtca gctccgacgt gtagtgcgac
    10741 ggcgcgacgc cgaagacgtc gaggaccacc gggacgaaga cgaccccctc ggtcgtcgtc
    10801 gtgccgccgg actgctcgac ctcgaacgac gccgtgccga tccggagcgt cccggtgcgg
    10861 ggggacgagt cgaggttcgg cgcgacggag taggtgagcg agccggagcc cgtgccgctc
    10921 cagcccgacg tgacgacgac ccacggcacc tcggacgtcg ccgtccaggg gcagcccgac
    10981 gagggcgtca cctggatcgt gccgcgcccg cccgccgcgc cgaacgcctg ggagaacggg
    11041 ggattgagcc ccgtgaagcc gcacgtgacg atccggaacg ccgcgtcgct catgtccgaa
    11101 gtcgtcccgg aggcgtcgct cacacggacg tggcagacgg gcgacatgga ctgcgcgacg
    11161 acccacggga acgtgccggt gttcggcgtg ctcgcgacga ccgtctgcgg atcgacgagc
    11221 tggccgtccc acggcgaggt cgttgagtac tcgatcttca cgttcgcgat ggatccgctc
    11281 gacgtccagg tgatcgggtg ggtcgtcccc gcctcccaga cctcgccgcc gttcggcgag
    11341 gtgacccgga gggtcggggc gccgccggag ccgaccgtca tcgagaagac gccgttcccg
    11401 ttcgtcccgg cgtagaggcg ggtgtggtcc gccggatcga gcgcgagggc caggacgtcc
    11461 aggttcgtga ggcccgagtc gaggtggttc cacgtggcgc caccgtccgc ggagaacgtc
    11521 acgccgtcgg acccggaggc gtagaacgtc cccggggagg cccggtccgc gacgagctcg
    11581 ccgtcggagc cccagaggtt cgtcacgcgg ctccaggccg ttccgccgtc cgtcgtccgg
    11641 tagacgccgt ccatcccgag gaggaggacc gtgctcgagg ctgaggggtg gacggcgatc
    11701 gcggagacga ggatcggaac ccctcccgag accgggctcc aggacgttcc gccgttcgtg
    11761 ctcttcatca gcccgccgaa cgtgctcgcc gcccagacgg tctgggggct cgacgggtcg
    11821 acggcgatcg cgttgacgaa catgtgcccg acacccgtgt agacgacgtt ccacgtcagg
    11881 ccgcggtcga tgctcttgta gacgtggtcc gtcgtcccgg cgtagacgac gagcggagac
    11941 gaggggtcga tcgcgaggca cgtcgtggcc tcgaggagga cgggcgtgta ggtggtcccg
    12001 ccgtcggcgc tccgcgccag ggcgcctccc gtcgccgcgt agacgatcga ggcgttcgcc
    12061 gggtcgaacg cgatcgccgc cgtggagtag ccggtcgaca cggcgctcca gctggcgccg
    12121 ccgttcctcg tgaccgagta gccggtcgac gtaccggcga cgatcgtcga ggagctgacg
    12181 ggggagacgg ccagcgccag aacctcggtc ccggccgggc cgatcgggct ccacgcgttc
    12241 tcgccggcgg agaggcgcgg tgcggcggcg acggcgagga cgagcgggaa gaggcccagc
    12301 gcggctcgcc gtgcaagggc gaaccggagt gtcgtgtcgt tcatcggggg ctccctcctg
    12361 gagcgggcgc gggcggagcg gcaatcacta ctcccgcgac gcgtctcgag ctgcaatgcg
    12421 gcacgaaacg atattggaga tgtcgcgggc ccgcaagaaa caggaccggc ccgggtcggt
    12481 cgggcgtcgc gtgacccggt gcgggagacc ggcggcaagt cggccgtggc cgagtcgaga
    12541 gtccgtctgc gggcgcgcgc gatcaggatc cggatcgtga tcccttcgcg ggagaggacc
    12601 ttcacagtgg gccatctcgt cgagggacgc gtgaggagtg acttgtggcg cacacttcca
    12661 ttcgtgattc gagcgttgct gtccgccgca ttgctggtgt cggggctggc cgccgcccag
    12721 ccctgcgcct ggccgtcgtt cgcccttccg gtcgacctcg gcattccggt cgaggagcgg
    12781 tggccgcagc tacggacagc agaagtggac ggggacggac gccaggatct cctcgcactc
    12841 ttcagctctg gccgcgtcgt cgtcttctgg aaccgcgccg gcggcctcgt tcggggcccc
    12901 tcgacggagc agctccctgg ctcagcgagc aggttcttcc tggagccggc ggacctcgac
    12961 ggggacggtc ggtcggacct tctcgtcggg gtgagctcgt tcggggcgcc gccgcggcga
    13021 ttcgagtcgt ggctcgggga cggggcgggc gggttctcga gggcggcgtc cgtcacgctc
    13081 cccgaggggc tcggctccgg tctcaccctc gcacggttcg acggaggcgc cacactcgac
    13141 gttctctctg tggttcgctc ggcgtccgac gcctcgaagc tcgaggtcgt gcgctggcgt
    13201 ggtcggggag acggatcgtt tcaggctccc gcgacgatcc tgacgctcga caatccgata
    13261 gccgacgccg gacccggcgg cgtcgtggcg ggcgacgtcg acggcgatgg aaggaccgat
    13321 ctcgtcgtct cctcgatcgg gagcgtgccc agccaccccg aggtccatcc cagctcctcc
    13381 tatgtgtcga agctctggct gggggacggc gcgggcggct tcgagcccag ctctcccgcg
    13441 cccttcggat gggctttccg gctcgtcgac gtcgatgggg acggtcggga cgaggtcctc
    13501 tatgccaact ggcagcagat gccggtcatc tcggactcag ggttcctgcg tcgagaggcg
    13561 gacgggacgt ggcgtgtcct aggaacgtgg ctcgccaatg cacagtccct cctcgtcggc
    13621 gagctcgaca ggaatcccga gcgcaaagag gttctcgtcg gtggaggcgc ctaccggctc
    13681 ggcgaagccg gcagcggtgc cgtccagttc gcggcgattc cggagaccgt gcagccgacg
    13741 ctgctggacc tggacggcga cggtcacctc gacgtcctcg gcgtcgcgag gaacccggtc
    13801 ggtgcctccg gccgcctcgt cctggcgcgg agcgtctgcg gaggggggcc gggaacgacg
    13861 acgctctttc ttcccgcgtt tctctccctg accggtgcgg aggcgacccg tttcgagacg
    13921 gaagtcaccg tcacgaactt cggggcctcc gcgatctcgg cggtcctgcg cattcacccg
    13981 gcggacggtg caccggcgtc ggacctggcc tccttcacgc tcgggccggg ccgggtccgg
    14041 ttgctctcca cgaccgaaga cgcggaggcg gtcagggtcg tgatgcccga aggtgtcgat
    14101 cggggcacgg cgacgctcga ggtctcgacg ctggacgggt cgcagcagga cgtcgtcgcg
    14161 gacgtccgga tactctcgga acggccgggt tccgggcgcg ggggcgtcgg ctatcgggcg
    14221 cgagccgccg aagggcagcg cgtcggggga accggatacg ttacctggct gaaggaagac
    14281 gcggaggatc gtacgaacct ggccgtcgcg agcgcaggcc aggaacccgt aaccctccgc
    14341 gtctccgtcg tctcggcgga tcccgaaaga ccaggccggg tcgtccttcc cgacgtcacc
    14401 ctgacgcctt acggaagcca ccagtggaac cgcgttctcg cggcgtccgg gctcggcgcc
    14461 cggagcggct gggtccacgt cgagcggctc gtcggaggtc catggacggc ctgggcgacc
    14521 gtgaactcga acggaaccgg agacggctcc gtcgtcgagg tggcggcgag cctggaggag
    14581 aggctcctcc ccgcgatcgt cgggacagat cgctaccgta cggacgtcgt catcacgaac
    14641 acggcggcga cgagcggact cgcaacgctc agcttcccgt ccgtctccga cccggatggg
    14701 ctcccgtttc cggtcacgct cgacatcgag gccggaccgg aatcgtcccg gctggtggac
    14761 ccggtctccg ctctgagaga cacgctcgag attccacccg agccgtatgt tgggcacgcc
    14821 accttccatg gttcgggtct cgtggccggg gcgagggtga cgaccgtgtc ggcatcctca
    14881 ggctcattcg gcgtctacac gcctcccgcg agctggttcg actacggcga gtccatgctc
    14941 gtctccggct tgcgccagga tcagagaaac cggtcgaacc tcgtgatcgt cgaatcgccc
    15001 accatggcgc cgggccggta tcgagtcgag ctgttcgacg ggcgcacggg cacgctggcg
    15061 aaggcgatcg aaggggtcac gcctggcgcc acccacggca tgccatcgcg ggtgcagctc
    15121 gactcggtcc tgtggggtac gggcgttccg ttcggctggg cacgcgtcac gcgcacctcc
    15181 tctttcaaca cccccttctt cgtctacgcc gtcgtcaacg acggcgcatc ggcggggctc
    15241 gggtcgggag acggaacgta cctgcccgga ataccgacgt gggcgcgcca gaccggggag
    15301 ccgtcgagga cgggggccgc cctaccggat cagctcgacg cgtcgggaca gacccggagc
    15361 gtgggcgagc ctggcatcgc aggtgaggag caccgtgtcg agcgcctcgg cgagcgcgac
    15421 gtagacggca tcgtaagcgg tcagcccgtc gcgcagggcc cagacccggc ccaggagcgg
    15481 ctcgtgaggg tggcgttccg ggtcgagcgc gcggaggtcc tcgagagtag acgtccccgc
    15541 ggccgcgtcg agctctccgg tcctcacgaa acgtcgcaag gcctgggtga cctcgacatc
    15601 ggccaggtgc gggacgtgga gaccgagcga ggggtcggcg attcgggccg cgatcgcgcg
    15661 gccgctcttc gtgccgagca ggagctcgac gagggccgac gcatcgagga cgatcaccgg
    15721 gcgttgcgct ccgtccggat cacggtcgcg gcggaccctc ggagccggcg caccggaccg
    15781 gcctggagcc gatcgaggag ctccgtccgc gtgggccgct cgagcgcctt gcggacctcg
    15841 cgcagaacgt agtccgacat cgtgagccct tcgaccgccg ccctcacctt gagccgacgg
    15901 tgcaggtcaa cggggacatt cctgatctgg accatggtcg tcggcatggg aaaacgatag
    15961 aaacctcagt acagcatgtc aacgctgtga tgccccgcgg tgagccgccg agccgcctct
    16021 catccctccg cctcgtaccc gaggttcggg gcgagccagc gctcggcctc gtggagcggc
    16081 atccccttgc gccgggcgta gtcggcgacc tggtcgcgac cgaggcggcc gacggcgaag
    16141 taatgcgcct cgggatgggc gaggtagagg ccggagaccg aggccgtcgg gagcatcgcg
    16201 tagctctcgg tgagggtgat gcccgcgttc gcggtggcgt cgaggagcga gaagagggtc
    16261 cgcttctcgg tgtggtcggg gcaggcgggg tagccggggg c
//
LOCUS       DAOONL010000078        15977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_3863_length_15977_cov_15.997990, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000078 DAOONL010000000
VERSION     DAOONL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15977
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3863_length_15977_cov_15.997990"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..732)
                     /locus_tag="PLP50_11695"
     CDS             complement(82..732)
                     /locus_tag="PLP50_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPA52253.1"
                     /translation="MTNYYDVLGVSRDASDEAIRNRFRALAREAHPDRVTDPAKKADA
                     EARFQLLTEAVNVLTNEQRRKAHDFDLNKGAGASTFDPAAIAKVYLAKGVKAYRDGDL
                     ATAFQQFDLSVHHWNKDAKALHYLALVCSRIPGKERRGVEAVEGALRLDGENGAVHRE
                     AARLYLQVGLRTKAERHLQEALRRIPEDGEVQRLAAELKPSSENRGLLGGLFGRKG"
     gene            complement(851..1900)
                     /locus_tag="PLP50_11700"
     CDS             complement(851..1900)
                     /locus_tag="PLP50_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52254.1"
                     /translation="MSADAPELDLLAAIESGQAPRRILEFAARGFVPLAPADLVRAVG
                     SILASGDPELAPLCEETFRGFDLESLHGAVRSPGARAEQLDAIAHRSKERRVLEGVLQ
                     HRAVSDATLVWLARRIPPDLQDVLVTNQARLLATPAIVENLFENPDLSADIRRRADEF
                     LEEFFLKKERERGERIEEAEEIADAEEIADAEEIADAEEVAEPERPAAAPEEESGDVL
                     FRLGSLTVAEKIRVAYRGNREERLFLVRDTNRLVSTAVLKSPKTNEADAEVIANMKSV
                     SEDVLRAIAQKREWMKKYSLMSAIVRNPRSPIDVTIGLVPRLSQRDQTTLATDRNVPE
                     VVRVTAKRICQRRQL"
     gene            complement(1932..2789)
                     /locus_tag="PLP50_11705"
     CDS             complement(1932..2789)
                     /locus_tag="PLP50_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA52255.1"
                     /translation="MAVETPQEFGEELRRERELRDVTREQLADVTKVSVRQIEALETG
                     RFEQLPARVFARGFVLALARHLGLDAERTAAAFNHVHEEWSAERERSAASTLSSSSDR
                     IRLSRPRKNASINTTALGVGVALVVAVLTFAAALLKGRAGDPAEEAGRIAAPVDAATP
                     VPQALGLPPAIAAATVPLSSDQPLVDPAVSRSVGASADAGRTLTLTFSDDCWTEVSVD
                     GRIVAAELFRKGTKRQFEGGRTFVLTLGNAGGVTVDVDGAALGAVGQPGQVVRNYVIG
                     EGADASRDG"
     gene            complement(2839..3645)
                     /locus_tag="PLP50_11710"
     CDS             complement(2839..3645)
                     /locus_tag="PLP50_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52256.1"
                     /translation="MNEPRRDGPDPGSFTAAISRADRATLAAFVEENLHRLGEADVLR
                     CLRHPYAGEVVIGLVLETSAFLSSREVRKAIVLHPGAPRPDALRLLEDLPWRDLVDVG
                     RDSRTPMPVRHAANRRLLEALPRLSRGERTAIARLADRALAGALLDDGDVDVLAALLG
                     NPRLVEEDLTAWLLVRQPQEAQLALLARSSRWMERTAVRSALLLSKRTPRALALSLLT
                     SSERPVWERLAGDPSADPLLAACARSLLEGADGAPARRRAGRSSGSSGGI"
     gene            complement(3653..4459)
                     /gene="trpA"
                     /locus_tag="PLP50_11715"
     CDS             complement(3653..4459)
                     /gene="trpA"
                     /locus_tag="PLP50_11715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPA52257.1"
                     /translation="MSRIGRAFSRAAREGRAALVVFVEAGDPSLETTERLLPALAAAG
                     ADVIELGVPFSDPIADGPAIQRASERGLAAGARLAAVLDLVARTRDAGFDAPVVLFGY
                     ANPVLSMGETAFASRAAAAGVDGALVTDLPPEEGAAFAATLKAVRLDPVFLLAPTSPP
                     KRVKTVGRLSRGFVYVVSRAGVTGVRSDVPEGLGELVGRVREGVGRLPIAVGFGISTP
                     AQVAAVARLADGVVVGSAVVLAMEEAARGGADPVEAAAALVWALAAATSR"
     gene            complement(4456..5667)
                     /gene="trpB"
                     /locus_tag="PLP50_11720"
     CDS             complement(4456..5667)
                     /gene="trpB"
                     /locus_tag="PLP50_11720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPA52258.1"
                     /translation="MAERFVVPEADAAGRFGEFGGRFVPETLMAPVEELTKAYAKARA
                     DASFRRELHHLLTTYAGRPTPLTEARRLSAELGARILLKREDLLHTGAHKINNALGQV
                     LLAKRMGKSRVIAETGAGQHGVATATAAALLGIPCVVYMGSVDVERQALNVYRMRLLG
                     TKVVPVDSGSRTLKDAINEAMRDWVASYADTHYVLGSALGPHPYPRMVRDFHAVIGRE
                     ARAQARKLLGGLPDLVVACVGGGSNAIGLFHAFLPEREVELVGVEAGGRSSAPGEHAA
                     RFGPGRVGVLHGTRTLLLADEDGQVLPTHSVSAGLDYPAVGPEHANLAALGRTRYESV
                     TDDEALAAFHVLSEKEGIIPALESAHAVAWVLREAPRRPGATIVLGLSGRGDKDVPTV
                     ARLEGTSGVGL"
     gene            complement(5660..7084)
                     /locus_tag="PLP50_11725"
     CDS             complement(5660..7084)
                     /locus_tag="PLP50_11725"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="monomeric bifunctional protein; functions in
                     tryptophan biosynthesis pathway;
                     phosphoribosylanthranilate is rearranged to
                     carboxyphenylaminodeoxyribulosephosphate which is then
                     closed to form indole-3-glycerol phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol phosphate
                     synthase/phosphoribosylanthranilate isomerase"
                     /protein_id="HPA52259.1"
                     /translation="MTAPSVLAEILAVRRRRLAAGELAPRGTAANPSDGGRFLAALRA
                     GGTRVIAEVKHRSPSAGVLLPVGPAPDTGLPAAIRSVAKAYRRAGAAAISVVTEPDFF
                     GGELAWLPEVKRDSGLPVLMKDFVVDEVQLDLALSLGADAVLLIVAALCDADLGRLHG
                     AARSRGLAVIVEAHDEEEVRRALAAGAEVVGLNARDLRTFAVDLGRVAAMGRLLPPSV
                     VRVAESGIHGPGDVARLAASGFDAFLVGESLLRSGDPARALRALRGEGTTEVKVCGVT
                     RGEDLAAAEELGADWIGLNVSTRSPRRVDLALGRALREASRFAKGVVAVVAGSTVAEA
                     EAIVDAVRPDALQWHDDLPARDGLRVPIPVWQAVRVGKDPLEEAAAWPADVLLLDAAH
                     ATLAGGTGLAWDWSLLASFPAKEKAFVAGGLSPQNVARAIAACAPAGVDVASGVESAP
                     GIKDEGKLAAFLAAVRSEKGRIGG"
     gene            complement(7081..8133)
                     /gene="trpD"
                     /locus_tag="PLP50_11730"
     CDS             complement(7081..8133)
                     /gene="trpD"
                     /locus_tag="PLP50_11730"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPA52260.1"
                     /translation="MSLPAAGAGDLHRALRLVMEGRTLSREEARLAVEEMLGEGFSAV
                     RAAALLAAMARRGEAAEEVAGVVDALRSRAARPPVADELAGRSVDVCGTGGDGQGTFN
                     VSTTAAFVVAGAGVPVAKHGNRAVSSASGSADVLAALGVRIEMPPTLAARALAEANVT
                     FLFAPAYHPAMKSVGPMRRELGIRTAFNLAGPLANPLGVTRQLVGVDRPLRVPVLAHA
                     LAALGAAHALVVSNSGAGDELLPHGLTIVAEVAEGEVRLEEWTAATFGLPERDPREMA
                     GGNAETNAGILRSILDGEEGPRRDAVLMNAAAALLVSGVALDLGDGMARAAASIDSGA
                     ARRALEGLSAISQEAA"
     gene            complement(8130..8699)
                     /locus_tag="PLP50_11735"
     CDS             complement(8130..8699)
                     /locus_tag="PLP50_11735"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPA52261.1"
                     /translation="MILVVDNYDSFTFNLVQFLQTLHPQVRVVRNDAMTAGEALALKP
                     ARIVISPGPGRPEDAGISEELIRRNEDVPLLGVCLGHQAIGHVFGASIVRAPVLMHGK
                     TSEIRHDGEGIYAGLGNPFVATRYHSLVVDRSTVPACLAITAETSDGVVMGLRHRERP
                     IVGVQFHPESILTLEGERLLANFVEKGAS"
     gene            complement(8696..10087)
                     /locus_tag="PLP50_11740"
     CDS             complement(8696..10087)
                     /locus_tag="PLP50_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPA52262.1"
                     /translation="MAKNEARTLVVLERPADLTTPVAVMRTLLSDDEPCFLLESVEGG
                     ERVARWSFLGTAPERSFSGADGDPFALLRAVVRSETRALPASDGFGDDLPPFIGGAVG
                     FAGYDAVRRLERLPSANPDVVGLPDAWFGVFETVLAFDHVRHRLLFLGNARPGEEAAV
                     RTRLATLAARVDAGRPHGGRPARLAGPWEESLPRKAYVDRVERAKEHIRAGDVFQVVL
                     SRRWSAPIEGDPFSIYRALRRVSPSPYQYYLRTPDAVIFGASPERLVRAGEEDVETIP
                     LAGTRRRGATPEEDLALARELLADEKERAEHVMLVDLGRNDLGRVAAPGTVKVRDFFT
                     VERFSHVMHLATRVTGRLADGKDAVDALAATFPAGTLTGAPKIRAMEVIEELEPVRRG
                     AYGGAVGYLDFAGHLDMAIGIRTAVVKDGVLHVQAGAGIVADSRPEAEADECESKAWA
                     LMRAVDAAKEYEP"
     gene            complement(10087..11508)
                     /gene="hisF"
                     /locus_tag="PLP50_11745"
     CDS             complement(10087..11508)
                     /gene="hisF"
                     /locus_tag="PLP50_11745"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPA52263.1"
                     /translation="MSLAVRVIPCLDVAVGRVVKGLKFENLRDAGDPVEAARRYEAEG
                     ADELCFLDVAASHEGRGTLVDLVRRVADVLSIPFTVGGGVRSVEDAGALLDAGADRVT
                     VNTAAVLDPSLVTRLAERFGAQCVVVAVDAKKDGGRSVVSTHGGRKVTEIELGAWVAE
                     VTARGAGEILLTSMDADGTKAGFDLPMLKAARAVATLPIVASGGAGEPGHFAPAVLEG
                     GADAVLAASVFHDRIFSVGMVKRAMAKAGVPVRPAIPAGLEEVAFDGRGLVPVVVRDA
                     TTGEVLTLAWANDEALALTVETRQTHLFSRSRKALWKKGETSGNVQRVVGVSLDCDRD
                     AVLYDVEPAGPACHTGAATCFAPIDAIPAGHAPAATRESADPFGLGPLFDVVAARKAS
                     PEPGSYTNRLLEKGIGKCAQKVGEEGVEVALAAVSRDDAGLAAEMADLMYHVVVLMAA
                     RGLEPSAVAAELAARRGARREEK"
     gene            complement(11505..12251)
                     /locus_tag="PLP50_11750"
     CDS             complement(11505..12251)
                     /locus_tag="PLP50_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPA52264.1"
                     /translation="MISLWPAIDLMGGKVVRLLHGDPSRRTVYPERAVEVALRFAREG
                     ADGLHVVDLDAAFGAGSNAGAIAEIVAASNVPVEVGGGLRDRASVEAALATPVARVVL
                     GSLPFRDPALFAAILADFSERLVVALDCKDGRPTVHGWVEDAGAGDAASAAAQLAARG
                     VKALLVTDVARDGAMTGPNLDLLAAVRAVFPGEVLASGGMRGEEDLAPVADALAGGPA
                     GAIFGRALHGGATTVARLAAARTALGKEMR"
     gene            complement(12248..12865)
                     /gene="hisH"
                     /locus_tag="PLP50_11755"
     CDS             complement(12248..12865)
                     /gene="hisH"
                     /locus_tag="PLP50_11755"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018979008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPA52265.1"
                     /translation="MTRVALLDYGVGNVGSVRRAFERLGAQVVTTSDPALVAESSRVV
                     LPGVGAFAPARARLASSGLDGALRAAVGRGARLLGLCLGFQLLFEASEEFGTTEGLGL
                     LPGRVVPFPPGVRVPHVGWNRLETEGAAEGPLLAGLPRGTYVYFVHSFRPEGAEPGDV
                     AAYCDHGGRFVAAARRGNVWGCQFHPERSSGAGRRILANFLSEAA"
     gene            complement(12862..13458)
                     /gene="hisB"
                     /locus_tag="PLP50_11760"
     CDS             complement(12862..13458)
                     /gene="hisB"
                     /locus_tag="PLP50_11760"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPA52266.1"
                     /translation="MNRSAEVSRATKETAIALALDVDGTGEARVSTPVGFLTHMLETV
                     AKHARFDLVVEATGDVHVDAHHTVEDVGLALGEALDRALGDRAGIARFGHSYVPLDEA
                     LARSVVDLSGRPYLVFEAPATPELQFVTREFPFALVEEFWKSFAFRGKLNLHVDVIRG
                     RNAHHAAEAVFKATARALRQAVALGEATVLSTKGTLTA"
     gene            complement(13455..14528)
                     /locus_tag="PLP50_11765"
     CDS             complement(13455..14528)
                     /locus_tag="PLP50_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPA52267.1"
                     /translation="MTRDPLAAVRPEIRALRAYRFEPGPRVVRAKLDFNESPADVPAP
                     AKAAALAALGARRFALYPEFGAPRLRAALAANAGLASEQVVPSNGSGEAILAALSVFA
                     GCGGTLLLAPPVFSLYFQMAAIVGAKVAAVPLAGDDFVLDEEAFLAAAAKGERTVPLL
                     CSPNNPTGGVVSRELVRRLCAVSDVVLVDQAYVDFAEGEDDLLPLLPELPNLVVFRTL
                     SKAWSIAGLRVGYAAARPDVADEIGKAILPFSVDVGAEEIALAAMAGREEVRARCRAI
                     AVERERVAAALRSLGAKVAPSRANFLFLVPPGGDAARVYAGLRARGVLVRDLSAAPPG
                     ALRVSIGTPEENDLFLSTLKEVL"
     gene            complement(14525..15847)
                     /gene="hisD"
                     /locus_tag="PLP50_11770"
     CDS             complement(14525..15847)
                     /gene="hisD"
                     /locus_tag="PLP50_11770"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPA52268.1"
                     /translation="MLRVAAAGGREGKALLARLAARRAAPDFATLREALPIVEAVLSG
                     GAPALAGFVRDLDGEALPEEGLLVKPPRERSVDPAFAAAFRLAKRRLTAYHRRQKPEG
                     FAFRDPLGVSFVEKPAPHEAVGVYVPGGRAFYPSSLLMGVVPARVAGVKRVVVATPPR
                     AFRGSRELRWALGEVGVDAVLLAGGAHGIAGLVSVAGCTKVVGPGNRWVAAAKHLVSG
                     VVSVDLPAGPSEVLILATEGADPTRIAADLVAQAEHDPDAVCLLFTDRRDLAVATARE
                     VERELEGLPTAETAKAAIAANGFALVFPSLEAAAKAGIPLAAEHVELTGRAAERYASR
                     FAATSGAVFVGAATPTAFGDYLAGPNHVLPTGGAARSFSGLSVRDFLRWGRSVSVPPA
                     AARRLGPAAAALARFEGLEAHARSLDLRCGGPLGGARATRERARRAGL"
BASE COUNT         2175 a         5878 c         5976 g         1948 t
ORIGIN      
        1 gagcaggtag gcgtcctcgt tgtgcttcct cttccggccg acgtcggtga gaccgtatgc
       61 ggtgagcgcc acggcgggat tctagccctt ccgcccgaac agcccgccga ggagcccgcg
      121 gttctcggag gacggcttga gctctgcggc gagtcgctgg acctccccgt cctccgggat
      181 ccgccggagg gcctcctgca ggtgacgctc cgccttcgtc cggaggccga cctggaggta
      241 gaggcgggcc gcctcccggt ggacggcgcc gttttcgccg tccaggcgga gggccccctc
      301 gaccgcctcg acgcccctcc gctccttgcc cgggatgcgg ctgcagacga gggcgaggta
      361 gtggagcgcc ttcgcgtcct tgttccagtg gtggaccgag aggtcgaatt gctggaacgc
      421 cgtggccagg tccccgtcgc ggtaggcctt gacccccttg gcgaggtaga ccttcgcgat
      481 cgccgcgggg tcgaacgtgg acgcccccgc ccccttgttg aggtcgaagt cgtgcgcctt
      541 ccggcgctgc tcgttcgtca ggacgttgac cgcttccgtc aggagctgga agcgcgcctc
      601 ggcgtcggcc ttcttcgcgg ggtccgtgac gcggtccggg tgcgcttccc gggcgagcgc
      661 gcgaaagcga ttgcgaatgg cctcgtccga agcgtcgcgg ctcaccccga ggacatcgta
      721 gtagttcgtc acggctttcc tgtcagctcg cgcagaccgg gcccccttgc gcagacggtg
      781 gcgaaattct cacacggtcc tcacgccaaa ctcaagcgcc gcgtcgtcgc ccggcagccg
      841 cccggtgggg tcagagctgg cgccgctggc agatccgctt cgccgtcacg cggaccacct
      901 cgggaacgtt ccggtcggtc gcgagcgtcg tctggtcccg ctgggaaagc cgcgggacga
      961 gtccgatcgt cacgtcgatg ggcgagcggg ggttcctgac gatcgccgac atgagcgagt
     1021 acttcttcat ccactcgcgc ttctgggcga tcgcccggag gacgtcctcc gagacgctct
     1081 tcatgttcgc gatgacctcg gcgtcggcct cgttcgtctt cggcgacttc aggacggccg
     1141 tggagacgag ccggttcgtg tcgcgcacga ggaagagccg ctcctcccga ttcccgcggt
     1201 aggcgacccg gatcttctcg gccacggtga gggagccaag gcggaagagc acgtcgcccg
     1261 actcctcctc gggcgcagcc gcaggccgtt ccggctcggc gacctcctcg gcgtcggcga
     1321 tctcctcggc atcggcgatc tcctccgcgt cggcgatctc ctccgcctcc tcgatccgct
     1381 ccccccgctc gcgctccttc ttgaggaaga actcctcgag gaactcgtcc gccctccgcc
     1441 ggatgtcggc cgagaggtcg gggttctcga agaggttctc gacgatcgcg ggcgtagcga
     1501 gcaggcgggc ctggttcgtg acgaggacgt cctggaggtc gggcggtatg cgccgggcga
     1561 gccagacgag cgtggcgtcc gagaccgcgc ggtgctggag gaccccctcc aggacgcgcc
     1621 gctccttcga ccggtgcgcg atcgcgtcga gctgctccgc ccgggcgccc ggcgaccgga
     1681 cggcgccgtg gagcgattcc aggtcgaagc cgcgaaacgt ctcctcgcag agcggggcga
     1741 gctccggatc accggacgcg aggatggagc cgacggcccg gacgaggtcg gccggcgcga
     1801 ggggcacgaa gccccgggcg gcgaactcga ggatgcggcg gggcgcctgc ccgctctcga
     1861 tcgcggcgag gaggtccagc tcgggagcgt ccgcggacat gcgggagtga gcttacgcca
     1921 gagcgatccg gtcagccgtc ccgcgacgcg tcggcgccct cgccgatcac gtagttccgg
     1981 acgacctgtc ccggctgccc gaccgcgccg agggcggcgc cgtccacgtc gacggtgacg
     2041 ccgccggcgt tcccgagcgt caggacgaac gtccggcccc cttcgaactg ccgcttcgtc
     2101 cccttccgga agagctccgc cgcgacgatc cgaccgtcga cgctcacctc cgtccagcag
     2161 tcgtcgctga acgtgagcgt cagggtgcgc cccgcgtccg cggacgcgcc cacggaacgg
     2221 gaaacggccg gatcgacgag aggctggtcg gacgagagcg ggaccgtggc cgcggcgatt
     2281 gccggaggaa gacccagggc ctgcgggacg ggcgtcgcgg cgtccacggg tgccgcgatc
     2341 cgcccagcct cctccgccgg gtctccggct cggcccttca ggagcgctgc cgcaaacgtc
     2401 aggacggcga ccaccagagc gactccgacc ccgagagccg tcgtgttgat cgaggcgttc
     2461 ttgcgcgggc gcgagagacg gatccggtcg gaggacgagc tgagcgtcga cgcggcgctt
     2521 cgctccctct ccgcggacca ctcctcgtga acgtggttga acgcggcggc cgtccgttcg
     2581 gcgtcgagcc cgaggtgtcg cgccagcgcg aggacgaacc ctcgtgcgaa gacccgcgcg
     2641 ggaagctgct cgaatcgtcc cgtctcaagc gcctcgatct ggcggaccga gactttcgtc
     2701 acgtccgcga gctgctcgcg cgtgacgtcc cggagctcgc gttcccttcg cagctcctcg
     2761 ccgaattcct ggggggtctc gacggccaac cgataaacct ccggggcaga agagattaca
     2821 tctcgcgtcg cggggatgtc aaattccgcc ggacgacccc gaggagcgcc ccgcccgcct
     2881 ccgggccggg gcgccgtcgg ccccctccag gagggaccgg gcgcaggccg cgagcagggg
     2941 gtcggccgac gggtcgccgg cgagtcgctc ccagacggga cgttccgacg acgtgaggag
     3001 ggagagcgcg agcgcgcgcg gcgtccgctt cgacaggaga agggcggagc ggacggccgt
     3061 ccgctccatc cagcgcgagg agcgcgcgag gagcgcaagc tgcgcctcct gcggctgccg
     3121 gacgaggagc caggccgtca ggtcttcctc gacgagcctc gggttcccga ggagcgccgc
     3181 caggacgtcg acgtcgccgt cgtccagaag ggctcccgcc agcgcccggt ccgcgagacg
     3241 agcgatcgcc gtcctctctc cccgcgagag ccggggaagc gcctcgagga ggcgccggtt
     3301 cgccgcgtgg cgaaccggca tcggcgtccg ggaatcgcgc cccacgtcga cgagatccct
     3361 ccaggggagg tcctcgagca ggcggagcgc gtcgggtcgc ggcgctcccg gatggaggac
     3421 gatggccttc cggacctcgc gactcgagag gaaagcggac gtctcgagga cgaggccgat
     3481 cacgacctcg cccgcgtacg ggtgtcgcag gcaacgcagg acgtccgcct cgccgaggcg
     3541 gtggaggttc tcctccacga acgcggcgag ggtggcccgg tccgcgcgcg agatcgccgc
     3601 ggtgaaggag ccgggatccg gcccgtcccg gcggggctcg ttcacccgtt cattatcggc
     3661 tcgtcgcggc ggcgagcgcc cacacgagcg cggcggccgc ctcgacgggg tcggcccctc
     3721 cgcgcgcggc ttcctccatc gcgaggacga ccgcgcttcc gacgacgacg ccgtcggcga
     3781 ggcgggcgac cgcggcgacc tgcgccggcg tcgagatgcc gaagccgacg gcgatgggaa
     3841 ggcgtccgac gccttcgcgc acccggccga cgagctcccc gagcccttcg ggcacgtcgg
     3901 acctcacgcc cgtgaccccg gcccgcgaga cgacgtagac gaacccgcgc gagagccggc
     3961 cgaccgtttt cacccttttc ggcggcgagg tcggggcgag gaggaagacc gggtcgaggc
     4021 gcaccgcctt cagcgtcgcg gcgaaggcgg ccccttcctc gggagggagg tcggtgacga
     4081 gcgcgccgtc cacgcccgcg gcggcggcgc gcgacgcgaa cgcggtctcg cccatcgaga
     4141 ggaccgggtt cgcgtagccg aagaggacga cgggcgcgtc gaaccctgcg tcgcgcgtgc
     4201 gcgcgacgag gtcgaggacg gcggcgaggc gcgcccccgc cgcgaggccc cgctcgctcg
     4261 cccgctggat cgcgggtccg tcggcgatcg ggtcggagaa cgggacgccg agctcgatga
     4321 cgtcggctcc tgccgccgcg agcgcgggaa ggaggcgctc cgtcgtctcg agcgacgggt
     4381 cgccggcctc gacgaagacg acgagggcgg cgcggccttc gcgggcggcg cgcgagaagg
     4441 cgcggccgat gcggctcaca gccccacccc cgaggtgcct tcgagccgcg cgaccgtggg
     4501 gacgtccttg tcgccgcggc ccgagaggcc gaggacgatc gtcgcgcccg ggcgcctcgg
     4561 cgcctcgcga aggacccagg cgacggcgtg ggcgctttcg agcgcgggga tgatcccctc
     4621 cttctcggaa agcacgtgga acgccgcgag cgcctcgtcg tcggtgacgc tctcgtaccg
     4681 cgtccggccg agcgcggcga ggttcgcgtg ctcgggcccg accgccgggt agtcgagccc
     4741 ggcggagacg gagtgcgtcg ggaggacctg cccgtcctcg tcggcgagga ggagcgtccg
     4801 cgtcccgtgg aggacgccga cacgccccgg cccgaagcgc gccgcgtgct cgcccggagc
     4861 ggaggaacgc ccgcccgcct cgaccccgac gagctcgacc tcccgctcgg gcagaaacgc
     4921 gtggaagagg ccgatcgcgt tcgagccgcc gccgacgcag gcgacgacga ggtcggggag
     4981 gccgccgagg agcttcctcg cctgggcccg cgcctcgcgg ccgatgacgg cgtggaagtc
     5041 gcggaccatc cgcgggtagg ggtgcggccc gagcgccgag ccgaggacgt agtgcgtgtc
     5101 ggcgtagctc gccacccagt cgcgcatcgc ctcgttgatc gcgtccttga gcgtcctcga
     5161 gcccgagtcg acggggacga ccttcgtccc gaggaggcgc atccggtaga cgttcagcgc
     5221 ctgccgctcg acgtcaaccg accccatgta gacgacgcac gggatcccga ggagcgcggc
     5281 cgcggtcgcc gtcgcgacgc cgtgctggcc ggcgcctgtc tccgcgatga cgcggctctt
     5341 tcccatccgc ttcgccagga gcacctgccc gagcgcgttg ttgatcttgt gcgcgcccgt
     5401 gtgaaggagg tcctcgcgct tgaggaggat gcgggcgccg agctccgccg agaggcgcct
     5461 cgcctcggtg agcggcgtcg ggcggcccgc gtaggtcgtc aggaggtggt ggagctcgcg
     5521 gcggaacgag gcgtcggcgc gcgccttcgc gtacgccttc gtcagctcct ccacgggcgc
     5581 catcaaggtc tcggggacga accgcccgcc gaactcgccg aaccgcccgg cggcgtcggc
     5641 ctcgggaacc acgaacctct cagccaccga tcctcccctt ctccgacctc accgcggcga
     5701 ggaacgccgc gagcttccct tcgtccttga ttcccggcgc gctctcgacg cccgaagcga
     5761 cgtcgacgcc cgccggcgcg cacgccgcga tcgccctcgc cacgttctgc ggcgagagcc
     5821 ctcccgcgac gaaggccttc tccttcgccg ggaagctcgc gaggaggctc cagtcccacg
     5881 cgaggcccgt ccctcccgcg agcgtcgcgt gcgcggcgtc gaggaggagg acgtcggcgg
     5941 gccacgccgc agcttcctcg agcgggtcct ttcccacccg gaccgcctgc cagacgggaa
     6001 tcgggacgcg aagcccgtcg cgcgcgggaa ggtcgtcgtg ccactgcagc gcatcgggcc
     6061 gaaccgcgtc gacgatcgcc tcggcctcgg cgaccgtgct ccccgcgacg acggcgacga
     6121 cccccttcgc gaagcggctc gcctcgcgca gcgcgcggcc gagtgcgagg tcgacccgcc
     6181 gcggcgagcg ggtcgagacg ttgaggccga tccagtcggc cccgagctcc tcggccgccg
     6241 cgaggtcctc cccgcgggtc acgccgcaga ccttcacctc cgtcgtcccc tcgccgcgca
     6301 gcgcacggag cgcgcgcgcc gggtcgccgg agcgaaggag cgactcgccg acgaggaatg
     6361 cgtcgaagcc cgaggcggcg aggcgcgcga cgtctcccgg cccgtggatg ccgctctcgg
     6421 cgacgcggac gacggacgga gggaggagcc ttcccatcgc ggcgacacgc ccgaggtcga
     6481 cggcgaacgt ccggagatcg cgggcgttca gcccaacgac ctccgcgccc gccgcgagcg
     6541 cgcggcggac ctcctcctcg tcgtgcgcct cgacgatcac cgcgagtccg cgcgagcgcg
     6601 ccgccccgtg gagccgcccg aggtcggcgt cgcagagcgc cgcgacgatg aggaggacgg
     6661 cgtcggcgcc cagcgagagg gcgaggtcga gctggacctc gtcgacgacg aagtccttca
     6721 tcaggaccgg gagacccgag tcccgcttca cctccgggag ccaggcgagc tcgcccccga
     6781 agaagtcagg ctccgtcacg accgagatcg ccgcggcgcc ggcacgccgg taggccttcg
     6841 cgacggaacg gatcgccgcc gggagtccgg tgtcgggggc cggacccaca ggcagcagga
     6901 cgcccgccga cggcgagcgg tgcttcacct cggcgatgac gcgcgttccg cccgcgcgga
     6961 gcgccgcgag gaagcgcccg ccgtccgagg ggttcgcggc cgttccccgc ggggcgagct
     7021 cgcccgcggc gagccgcctc cgccgcacgg cgaggatctc cgcgaggacc gacggcgcgg
     7081 tcacgccgcc tcctgcgaga tcgcggagag gccttccagc gccctgcgcg cggcgcccga
     7141 gtcgatcgag gcggcggcgc gggccatccc gtcgccgagg tcgagcgcga cgcccgagac
     7201 gaggagcgcc gcggcggcgt tcatcaggac ggcgtcccgc cgcggtccct cctcgccgtc
     7261 gaggatcgag cggaggatcc cggcgttcgt ctcggcgttc ccgcccgcca tctcgcgcgg
     7321 gtcgcgctcc ggaaggccga acgtggcggc cgtccactcc tcgaggcgga cctccccttc
     7381 cgcgacctcg gccacgatcg tcaggccgtg cgggaggagc tcgtctcccg cacccgagtt
     7441 cgagacgacg agcgcgtgcg cggcgccgag cgcggcgagc gcgtgggcga ggaccggcac
     7501 gcggagaggc cggtcgacgc cgacgagctg gcgcgtgacc ccgagcgggt tcgcaagcgg
     7561 cccggcgagg ttgaaggccg tccggatccc gagctcgcgc cgcatcggcc cgacggactt
     7621 catcgccggg tggtaggccg gggcgaagag gaacgtgacg ttcgcctcgg cgagagcgcg
     7681 ggccgcgagg gtcggtggca tctcgatcct cacgccgagc gcggcgagga cgtccgccga
     7741 gccgctcgcc gaggagacgg cgcggttgcc gtgcttggcg accggaacgc ccgcccccgc
     7801 gacgacgaaa gcggccgtcg tggagacgtt gaacgtcccc tgtccgtcgc cgcccgtccc
     7861 gcagacgtcg acggatcgac cggcgagctc gtccgcgacc ggaggccgag ccgcgcggga
     7921 gcggagcgcg tcgacgaccc cggcaacctc ctcggccgcc tccccccgcc gcgccatcgc
     7981 cgcgaggagg gccgcggcgc ggaccgcgga gaaaccctcc ccgagcatct cctcgacggc
     8041 gaggcgcgcc tcctcccgcg agagcgtccg gccctccatg acgagcctca gcgcgcggtg
     8101 aaggtcgccc gcgcctgccg cggggaggct cacgacgcgc ccttctcgac gaagttcgcg
     8161 aggagccgct ccccctcgag cgtgaggatc gactcggggt ggaactggac accgacgatc
     8221 ggccgctcac ggtgccggag gcccatgacg accccgtccg aggtctccgc cgtgatggcg
     8281 aggcaggcgg ggaccgtcga ccggtcgacg acgagggagt ggtagcgcgt cgcgacgaac
     8341 gggttcccga ggccggcgta gatcccctca ccgtcgtggc ggatctcaga ggtcttcccg
     8401 tgcatcagga ccggcgcccg gacgatcgag gcgccgaaga cgtgcccgat cgcctggtgg
     8461 ccgaggcaga cgccgagaag cggcacgtcc tcgttccgcc ggatcagctc ctcgctgatc
     8521 ccggcgtcct cgggacggcc cggcccgggc gagatcacga tccgcgccgg cttcagcgcg
     8581 agcgcctccc ccgcggtcat cgcgtcgttc ctcacgacgc ggacctgggg gtggagcgtc
     8641 tggaggaact ggacgaggtt gaaggtgaac gagtcgtagt tgtcgacgac gaggatcacg
     8701 gctcgtactc cttcgccgcg tcgacggcgc gcatgagcgc ccacgccttg ctctcgcact
     8761 cgtccgcctc ggcctcgggg cgggagtcgg cgacgatgcc ggcgcccgcc tggacgtgga
     8821 ggacgccgtc cttcacgacc gccgtccgga tgccgatcgc catgtcgagg tgtcccgcga
     8881 agtcgaggta gccgacggcg ccgccgtagg ccccgcgacg gaccggctcg agctcctcga
     8941 tgacctccat cgcccggatc ttcggggcgc ccgtgagcgt ccccgccggg aaggtcgccg
     9001 cgagggcgtc gacggcgtcc ttgccgtcgg cgaggcgtcc ggtgacacgc gtcgcgaggt
     9061 gcatgacgtg cgagaagcgc tcgacggtga agaagtcccg gaccttgacg gtccccggcg
     9121 ccgcgacgcg gccgaggtcg ttccggccga ggtcgacgag catcacgtgc tcggcccgct
     9181 ccttctcgtc ggcgaggagc tcgcgcgcga gggcgaggtc ctcctccggc gtcgccccgc
     9241 ggcggcgcgt ccccgcgagc gggatcgtct cgacgtcctc ctcgccggcg cggaccagcc
     9301 gctcgggcga agctccgaag atcaccgcgt cgggggtccg aaggtagtac tggtagggcg
     9361 acgggctgac acgccgcagg gcgcggtaga tcgagaaggg gtccccctcg atcggcgccg
     9421 accagcggcg cgagaggacg acctggaaga cgtcgcccgc gcggatgtgc tccttcgccc
     9481 gctcgacgcg gtcgacgtag gcctttcgcg ggagcgattc ctcccagggg cccgcgaggc
     9541 gcgcggggcg cccgccgtgc ggccgtccgg cgtccacccg cgcggcgagc gtcgcgaggc
     9601 gggtcctcac ggccgcctcc tcgccggggc gcgcgttccc gaggaagagg agccggtgcc
     9661 tgacgtggtc gaatgcgagg accgtctcga agacgccgaa ccaggcgtcg ggcaggccga
     9721 cgacgtcggg gttcgccgac gggagccgct cgagacgcct cacggcgtcg tagccggcga
     9781 agccgaccgc cccgccgatg aacggcggca ggtcgtcgcc gaaaccgtcg gaggcgggga
     9841 gggcgcgcgt ctccgacctc acgacggccc ggaggagcgc gaacgggtcg ccgtcggcgc
     9901 cgctgaagga gcgctccggg gcggtcccga ggaacgacca gcgcgccacg cgctctcccc
     9961 cttcgaccga ctcgaggagg aagcagggct cgtcgtccga gaggagcgtc cgcatgacgg
    10021 cgaccggggt cgtcaggtcg gccggcctct cgaggacgac gagcgtccgg gcttcgttct
    10081 tcgccatcac ttctcctccc ggcgcgcgcc gcgccgtgcc gccagctccg ccgcaaccgc
    10141 ggagggctcg agcccccggg cggccatcag gacgacgacg tgatacatca ggtcggccat
    10201 ttccgccgcg agccccgcgt cgtcgcgcga gactgccgcg agggccacct cgaccccctc
    10261 ttccccgacc ttctgcgcgc acttcccgat tcccttctcg aggagccggt tcgtgtacga
    10321 gcccggctcg gggctcgcct tccgcgccgc cacgacgtcg aagaggggac cgaggccgaa
    10381 cgggtcggcg ctctcgcggg tcgccgcggg tgcgtgtccc gcggggatcg cgtcgatcgg
    10441 cgcgaagcag gtcgcggccc ccgtgtggca ggccggcccg gccggctcga cgtcgtagag
    10501 gacggcgtcg cggtcgcagt cgagcgacac gccgacgacc cgctggacgt tccccgaggt
    10561 ctcccccttc ttccagagcg ctttccgcga cctgctgaag aggtgcgtct ggcgggtctc
    10621 gacggtgagc gcgagcgcct cgtcgttcgc ccaggcgagc gtcaggacct cgcccgtcgt
    10681 ggcgtccctc acgacgacgg ggacgagccc tcggccgtcg aacgcgacct cctcgagccc
    10741 cgcggggatc gcggggcgga ccgggacgcc tgccttcgcc atcgcccgct tcaccatccc
    10801 gacgctgaag atccggtcgt ggaagaccga cgccgcgagg acggcgtcgg cgcccccctc
    10861 gaggaccgcc ggcgcgaagt gcccgggctc ccccgcgccg ccggaggcga cgatcgggag
    10921 cgtcgcgacg gcgcgggccg ccttcagcat cgggaggtcg aagcccgcct tcgtcccgtc
    10981 ggcgtccatc gacgtgagga ggatctcccc cgccccgcgg gcggtcacct cggcgaccca
    11041 ggctccgagc tcgatctccg tgaccttccg gccgccgtgg gtcgagacga cggaacggcc
    11101 gccgtccttc ttcgcgtcga cggcgacgac gacgcactgc gcgccgaagc gctccgcgag
    11161 gcgcgtgacg agcgaagggt cgaggacggc cgcggtgttc acggtgacgc ggtcagcccc
    11221 ggcgtcgagg agcgcgccgg cgtcctcgac ggagcgaacg ccgccgccga cggtgaacgg
    11281 gatcgagagg acgtcggcga cgcggcgcac caggtcgacg agcgtgccgc gcccctcgtg
    11341 cgaggcggcg acgtcgagga agcagagctc gtcggctcct tccgcctcgt agcggcgcgc
    11401 ggcctcgacg gggtcgcccg cgtcgcgcag gttctcgaac ttcagtccct tgacgacgcg
    11461 tcccaccgcc acgtcgaggc acgggatgac ccggacggcg aggctcatcg catctccttt
    11521 ccgagggcgg ttcgggccgc cgcgaggcgg gcgaccgtcg tcgctccccc atgcagggcc
    11581 cggccgaaga tcgcccccgc ggggccgccg gcgagggcgt cggccacggg cgcgaggtct
    11641 tcctcgccgc gcatcccgcc cgaggcgagg acctcgcccg ggaagacggc gcggaccgcg
    11701 gcgaggaggt cgaggttcgg ccccgtcatc gcgccgtcgc gcgcgacgtc ggtgacgagg
    11761 agcgccttca cgccccgggc cgcgagctgc gcggcggcgg aggcggcatc tcccgccccg
    11821 gcgtcctcca cccagccgtg gaccgtcggg cgcccgtcct tgcagtcgag ggcgacgacg
    11881 aggcgctcgc tgaagtcggc gaggatcgcc gcgaagaggg cgggatcgcg gaaagggagg
    11941 ctcccgagga cgacgcgcgc gacgggtgtc gcgagcgcgg cctcgacgga ggcccgatcc
    12001 ctcagtcccc cgccgacctc gacgggaacg ttcgaggccg cgacgatctc cgcgatcgct
    12061 ccggcgttcg acccggcgcc gaaggcggcg tccaggtcga cgacgtggag gccgtcggcc
    12121 ccttcgcgcg cgaagcggag ggcgacctcg acggcgcgct ccgggtagac ggtgcgccgc
    12181 gacgggtcgc cgtggaggag acggacgacc ttcccgccca tcaggtcgat tgcgggccag
    12241 agggagatca cgcggcctcc gagaggaagt tcgcgaggat cctccggccg gcgccggaag
    12301 acctctcggg gtggaactgg cagccccaga cgtttccccg gcgggccgcg gcgacgaagc
    12361 ggccgccgtg atcgcagtag gccgcgacgt ctcccggttc ggccccctcg gggcggaacg
    12421 agtggacgaa gtagacgtac gtcccgcgcg gcagtcccgc gaggagcggc ccctccgccg
    12481 cgccctcggt ctcgagccgg ttccagccga cgtgcggcac gcggacgccc ggcgggaacg
    12541 ggacgacgcg gcccgggagg aggccgagcc cctcggtcgt cccgaactcc tcgctcgcct
    12601 cgaagaggag ctggaagccg aggcagaggc cgaggaggcg cgcgccgcgg ccgacggcgg
    12661 cgcggagtgc gccgtcgagg cccgacgagg cgagtcgcgc gcgggccggg gcaaaggcgc
    12721 cgacgcccgg caggacgacc cgcgacgact ccgcgacgag cgccgggtcg gaggtcgtga
    12781 cgacctgcgc gccgagccgc tcgaaggcgc ggcggacgga gccgacgttc ccgacgccgt
    12841 agtcgaggag ggcgacgcgc gtcatgccgt gagcgtcccc ttggtcgaga ggaccgtcgc
    12901 ctcgccgagc gccacggcct ggcggagcgc ccgggccgtc gccttgaaga ccgcctccgc
    12961 cgcgtggtgg gcgttccggc cgcggatcac gtcgacgtgg aggttcagct ttccgcggaa
    13021 cgcgaacgac ttccagaact cctcgacgag ggcgaacggg aattcccgcg tcacgaactg
    13081 gagctccgga gtcgcgggag cctcgaagac gaggtacggc cgccccgaga ggtcgacgac
    13141 gctccgcgcg agagcctcgt cgagcggcac gtaggagtgc ccgaagcggg cgattcccgc
    13201 gcggtcgccg agggcgcgat cgagggcctc gccgagcgcg aggccgacgt cttcgacggt
    13261 gtggtgcgcg tcgacgtgga cgtcccccgt cgcctccacg acgaggtcga agcgcgcgtg
    13321 cttcgccacc gtctcgagca tgtgcgtcag gaacccgacc ggagtcgaga ccctcgcctc
    13381 gcccgtcccg tcgacgtcga gcgcgagcgc gatcgcggtc tccttcgtcg cccggctcac
    13441 ctcggcggaa cggttcacag gacctccttc agggtcgaga ggaagaggtc gttctcctcc
    13501 ggcgtcccga tcgagacgcg cagcgcaccc ggcggggcgg ccgacaggtc gcggacgagg
    13561 accccgcggg cgcggaggcc ggcgtagacg cgcgcggcgt ctccgcccgg cgggacgagg
    13621 aagaggaagt tcgcccgcga cggcgcgacc ttcgcgccga gcgagcggag cgccgccgcg
    13681 acccgctcgc gctcgacggc gatcgcgcgg cagcgggcgc ggacctcctc gcgccccgcc
    13741 atggccgcga gggcgatctc ctcggcgccc acgtcgacgc tgaacggcag gatcgccttc
    13801 ccgatctcgt ccgcgacgtc gggccgggcg gccgcgtacc cgacgcgcag gccggcgatc
    13861 gaccacgcct tcgagagggt ccggaagacc accaggttcg gcagctcggg gaggagcggg
    13921 aggaggtcgt cctcgccttc ggcgaagtcg acgtaggcct ggtcgacgag gacgacgtcc
    13981 gagacggcgc agaggcgccg gacgagctcg cgcgaaacga cgccgcccgt cggattgttc
    14041 ggcgagcaga ggagcggcac ggtccgctcc cccttcgccg ctgccgcgag gaaggcctcc
    14101 tcgtcgagca cgaagtcgtc accggcgagg ggcacggccg ccaccttcgc cccgacgatc
    14161 gcggccatct ggaagtagag cgagaagacg ggcggcgcga ggaggagcgt cccgccgcag
    14221 ccggcgaaaa cggagagcgc ggcgaggatc gcctcgcccg acccgttcga cgggacgacc
    14281 tgctccgagg cgaggcccgc gttcgcggcg agcgcggcgc gaaggcgagg cgccccgaac
    14341 tccgggtaga gcgcgaagcg ccgggcgccg agcgccgcca gggcggcggc cttcgccggc
    14401 gccgggacgt cggcggggct ctcgttgaag tcgagcttcg cgcggacgac ccgcggcccc
    14461 ggctcgaagc ggtaggcgcg gagggcgcgg atctcgggcc gaacggcggc gagcgggtcg
    14521 cgggtcacag accggccctc cgcgcccgct cccgtgtggc gcgcgcccct ccgagcgggc
    14581 cgccgcaacg gaggtcgagc gaacgcgcgt gcgcctcgag cccctcgaag cgcgcaagcg
    14641 ccgcggcggc ggggccgagc cgcctcgcag cggccggcgg gacggagacg ctccggcccc
    14701 agcgaaggaa gtcgcggacg gagagccccg agaagctccg cgccgcgccc cccgtcggga
    14761 ggacgtggtt cggccccgcg aggtagtcgc cgaaggcggt cggcgtcgcg gcgccgacga
    14821 agacggcgcc ggaggtcgcg gcgaagcgcg acgcgtacct ctcggcggcg cgccccgtca
    14881 gctcgacgtg ctcggcggcg agcggaattc ccgccttcgc ggccgcctcg aggctcggga
    14941 agacgagggc gaagccgttc gccgcgatcg cggccttcgc ggtctccgcc gtcggcaggc
    15001 cctcgagctc ccgctcgacc tccctcgccg tcgcgacggc gaggtcgcga cggtcggtga
    15061 agaggaggca gaccgcgtcc gggtcgtgct cggcctgggc gacgaggtcg gccgcaatcc
    15121 tcgtcgggtc ggcgccctcc gtcgcgagga tcaggacctc ggacggcccg gccggcaggt
    15181 cgacggagac gacgccggag acgaggtgct tggccgccgc gacccagcgg ttcccggggc
    15241 cgacgacctt cgtgcagccg gcgaccgaga cgagcccggc gatcccgtgc gcgccgccgg
    15301 cgaggaggac cgcgtcgacg ccgacctcgc cgagtgccca gcggagctcg cggcttccgc
    15361 gaaaggcgcg aggcggcgtc gcgacgacga cccgcttcac gcccgcgacg cgcgccggga
    15421 cgacgcccat caggagcgac gacgggtaga acgcccgccc gcccgggacg tagacgccga
    15481 cggcctcgtg cggcgccggc ttctcgacga acgagacgcc gagggggtcg cggaaggcga
    15541 agccctcggg cttctgtcgg cggtgatacg ccgtcaggcg cctcttcgcg aggcggaacg
    15601 ccgcggcgaa ggccgggtcg accgaccttt cgcgcggcgg cttcacgagg agcccctcct
    15661 ccggcagcgc ctcgccgtcg aggtcgcgga cgaaacccgc gagcgcgggc gcgccgcccg
    15721 agaggaccgc ctcgacgatc ggcagcgcct cgcggagcgt cgcgaagtcg ggcgcggcgc
    15781 gacgcgcggc gagtcgcgcg aggagcgcct tcccctcgcg cccgcccgcg gcggcgaccc
    15841 tcaacacggg ctttccacct tggcagcgag gcgggagacg agcgcggcca cctcggcacg
    15901 gcgctcgacg agcgcccgcg ggccgacgac gaacgtcgcg cggctcggga ggatcgtctc
    15961 gatctcggcg aggccgt
//
LOCUS       DAOONL010000079        15362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4102_length_15362_cov_16.830143, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000079 DAOONL010000000
VERSION     DAOONL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_15362_cov_16.830143"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2293)
                     /locus_tag="PLP50_11775"
     CDS             complement(<1..2293)
                     /locus_tag="PLP50_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HPA52269.1"
                     /translation="MKRAALLALLAALPAAAQTGLLVPTSTGRPDARVLTLREMAVDV
                     GVARGYARVNVRQVFENHTGEVQEGTWRFRLSPSGAVGDFAVWDGLVRIPGVIVEKKR
                     ARALYEELTTRRIDPGLLQQGEEEDASSGGPGRPSGGAVFSVKVAPIPGYGTKRLELQ
                     YQEEVPWADGVGELLIPLRPGAGEPAVAGSLEVNVTLEDGAFLEAPSTALPLAVSGKV
                     ARFRGSAVPLAKDVVVRFRPASSAPLTFSAFRSPDGRLPDGVALAPWERLADVPPEKD
                     GFFLLEHRPAAASGTSVADARGRAPLRLAVLFDTSLSSRWGSLETGWAFLTRLLDRLG
                     PEDRFVLVPFDRESAPDPAGLVAATPAAKEAALSYLRARPLSPGTDVASALRAAAALV
                     PSGSDARLVLVTDGVEAPGALAASAAGRPLFTVLTGEERREAFVVSSRQLLLLPPGSA
                     LQAPPGEALFLAQLFAPLPKEEPREGSASRLPFARKGEDPELRDVYGVMTQPPLAGSL
                     SGWVGRYARPAKATIELGGASVEARFPERSLDARDLPRRWARARVDDLLRRIELEGEK
                     REWVEEILELSRRYKLVTPYTAFLAAPRALLRPRRIQPGDPVIRIDALPGTAAVAALL
                     PFGRRLDLLRRPGSTLWEGRFLVPDGTKDGPMDVRIVVRDRNGATAVESKRVIVDGTP
                     PTVTPVLPSSAAAGTRVRLAARADADVIALTARVGDGAPVPLRWDGAAKANAAELFLP
                     QGLTGDVPVHFEAIDGAGNHGFAR"
     gene            complement(2290..4140)
                     /locus_tag="PLP50_11780"
     CDS             complement(2290..4140)
                     /locus_tag="PLP50_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52270.1"
                     /translation="MSPAARRALLLAGGAFLAASAAALLLLFRVDRAVTRAAAPEALR
                     AESVRPLGFEALSPTPVAGDAAGDGAVLGVAEASGELLLATGSGLEAGGRTLGVLDGL
                     PSLRVGAVASWRGTPVFALERGGWGRLAPSGPERAVSGFDPLEVRSFAETDGGELLLG
                     ARQGLFRASFASGELEKLDDASVLSLALLPTGEVAVGGESGLRIVAPGRAEARLVATP
                     DRWVSRVAVVDGKLWVATATGAALGPLEATTLTAHPRGADAVDGVAFDGAFRFVPTDD
                     PPRLASLLPDGTRREVPAPERFRRLFVAGGQLLADGPTGLRRHDPARGFELVREASPG
                     LPAPHVNALAADGARLWLGFFDGGLATADPGASPLRASPAPSGGAWGVNAILPVAGVV
                     WAATLRGAFRIEGEDVRAVDGAGAAFALAQTTSGLAIGYGQGVLLPARRLLSAWHGLP
                     GNQAYALAADGSSLWVGTPTGLGRVERQRVAWRVLAGEGKLPHPWVTALFAGDDGLFI
                     ATYGGGIAVRRRGGADAPFEPFPETRGLRVNAGALLALPDGRLCAGTQGSGVWCSDRA
                     RTRFARLDLPLPSPDVFSLAAFPKEAPEHFFAGTSEGLLRLPADALPETR"
     gene            complement(4193..5644)
                     /locus_tag="PLP50_11785"
     CDS             complement(4193..5644)
                     /locus_tag="PLP50_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPA52271.1"
                     /translation="MDLTELLKFVTKKEASDLHLKPGRPPLLRINGRLVPLKTDPLQP
                     KEIEDMVLTILTPHQKEKLTANYAVDIGYGIQGLARFRGNIFLQRGSWSAVFRRIPFT
                     IPGVEELHLPDVIQTFCDYPSGLVLVTGPTGSGKSTTLAGIIKGITERHPVHVVTVED
                     PIEYLFQDNLGSVCQREVGTDTVSFHEALRNAMRQDPDVIMVGEMRDWETMQTAITAA
                     ETGHLVFSTLHTNNAAQTIDRILDSCPGGQQNQVRRQLALVLRSICSMQLIERIDGQG
                     RVPVIEILVNSPKITKLIEEGHSNEILDEMESSVTYYRMQSMNQSLLALLVNGAIDYE
                     KAMQLTADPEDLSLKLRKLFPQIEEKFRGGIMAPSANDFSAITELMDIKKLYEEAEEK
                     WKLRLQEKDDLIANLEADLQSKDRVLEANSMGSQELESELQKARQENERVRQEAAARI
                     AQLNERIKELNNRIMGGGNPPSQGTPQSGFFKR"
     gene            complement(5667..6920)
                     /locus_tag="PLP50_11790"
     CDS             complement(5667..6920)
                     /locus_tag="PLP50_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52272.1"
                     /translation="MSLLQEPRAALARLARRSAVRLARTASGRSGTPFLVVGGAVRDA
                     LLGLPAGDLDLAVSRDGAARFAAALARLAGTRVVPIGREPRRILHVPVRRSSVDIWET
                     EGDPADDLLRRDFTVNALALAFPGARLVAPEGALDDLAARRLRLPRPGVLLEDPLRVV
                     RAARFLARLPGFRLDPGMRTELAVGARGLGAVAPERRLAELDALLAVGSARAARALGQ
                     LETWGGLAALLPGTDVEERRRGLAALAAVRRDAQPALLRAVLLSGAPTERGAAVLDAL
                     RASRNDRRLAATLAGLPAPSARPDATEAVRLLRRAAPFSREAIAFVEATGGPRGRALA
                     RCVDAVLAPPGALGRLLRPRRPLPVSVVAVLTGATGPQLGRALERLDDALATGAIRGA
                     GAARAFLSGSPRPDARRGRRTRRTV"
     gene            6984..7277
                     /locus_tag="PLP50_11795"
     CDS             6984..7277
                     /locus_tag="PLP50_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HPA52273.1"
                     /translation="MRIDGGLVRRMARLAALDLAEEEAGRLAEELRRVLDHFEVIRSV
                     PDDLLPPLSDAPAALLRDDVPSAEAGERPFAVANAPEMARGHFVAPRVVSRNG"
     gene            7274..8746
                     /gene="gatA"
                     /locus_tag="PLP50_11800"
     CDS             7274..8746
                     /gene="gatA"
                     /locus_tag="PLP50_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPA52274.1"
                     /translation="MTGEADRLLDGPAHRLAVAVSTREVRARDVTGAALGRAARSRER
                     HGAFLHVAGEDAIEAAAEVDRRIAAGETFPLAGVPIAVKDNVHVAGMPTTAGSRVLGA
                     IVPPCDATCVAQLRAAGCVVVGKTSCDELGMGSSNETSAFGPVRNPWNPDRVPGGSSG
                     GSAAAVAARAVPLAVGTDTGGSIRLPAAFCGLVGLRPTYGRVSRSGLVAYASSFDQAG
                     PIARDVRDAALALAAMAGADPSDATSLPGPPPDLLSRLDAGVAGLRVGLLDHAGEGVD
                     PDTEAALASTAGLLASRGASVVPLRLPRLSLGIAAYYLISAAEASSNLARFDGVRFGA
                     RVAGDGPGALVRATRAAGFGDEVKRRILLGTFALSAGHREAWYERAQRVRARIALDLA
                     DAFRACDLLLSPTAPGPAFPIGEKSGDPLSLYLTDVFTVPASLAGLPALSVPAGLSRE
                     QLPVGVQLAGPAGSEALLLGVARELEAAAGPRPDPPGVIR"
     gene            8743..10635
                     /locus_tag="PLP50_11805"
     CDS             8743..10635
                     /locus_tag="PLP50_11805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPA52275.1"
                     /translation="MRGALSRAALAALLLVPFLGQAQAALRRVEVSEDFSAYLDQGSI
                     LVEVRPLDGESVTDFARRISRDEAAAARLLALPAAPSRSRRIVLPYGDLSDETRLAAV
                     RALFPADVRATIGWLHVAVETEPLASIATWFTGDARLASRLAELNGLSGSEPVTRGTL
                     VKIPVEYLLPPFRDAEPIPDEEPPRLEYGSDEKGRYAVYRLKRKEALYSAVVVRFTGR
                     IHAEDVIQLALDIALRSGIDDVHAIPVGYPVKIPLELLSEEYLPPDDPKAAAQAKERA
                     EAALFATPAKALFLAGVRVVIDAGHGGTDTGTLHHGVWESTYVYDVASRLRKVLLETT
                     RAEVVMMTREPGLGWEVLDRDRIPDRKGRVLLTDPPYKMTNVVAGVNLRWYLANAILK
                     RPGPGGKPIPPEKTVFLSLHADSLHPSVRGAMAYVPGERFLRDRYGRATEFYRRFREV
                     RDDPVVSFTKKERVLAEGASTSLAEKLIGAFRRRSLPVHQFRPVRTHVIRKGREWVPA
                     VLRYNKIPNRVLFELSNLSNEEDRRLTLTRAYRQECAEALADGLVAFFGGAADERGAS
                     PSTAAGRQAPRVAAAPAPAELPGPWPDTIGPWPEGAPQGRTALVPSPTPTPTKTPRKP
                     VRRRRR"
     gene            complement(10640..11551)
                     /locus_tag="PLP50_11810"
     CDS             complement(10640..11551)
                     /locus_tag="PLP50_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA52276.1"
                     /translation="MQPSPAARSRALLGVFLCVLVWGTNYPATKIAYRELSPLAYTGW
                     RFLLAAALILAWARRNHAPVLPPRGLRFPGFLLAMTGVGVYQLFFAIGVAKTSGFAAA
                     LLNSVSPLLSLLLVALLGQEKIPRTAVVGTVVAWGGVLAFLSSAHGSADLGGLTGNLL
                     CLVSATCWSVYSVLSSRYSTRIPHATALASTFTFGVPVLLAYCLPAMLRQDYSSVSTA
                     AWVILVLSAVFPLYVSFRLWAHGLAVLGVAATTRIGVLVPLVAGVASALWTGERFSGG
                     KVVSAAVVLGGLALARLGTSRETPDKA"
     gene            11647..12651
                     /locus_tag="PLP50_11815"
     CDS             11647..12651
                     /locus_tag="PLP50_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA52277.1"
                     /translation="MLEVEALRKSFYGTLAVDDVSFRVESGEILGFLGPNGAGKTTTM
                     RMITGFLEPNAGRVTVDGVDAAKKPREARARIGYLPETLALYPEMRVREYVRFRAELC
                     GVPRRETKARVDDALATCFVDDVASKPIGNLSKGYRQRVGLAAALVHAPQVLVLDEPT
                     VGLDPRQIVKVRDLIRDLKKDHTILLSTHILPEVELVCDRVVIIDRGKIVATGTPETL
                     RQQLTQAPGLAVAIQGEVPDADGILRKLPGVVRAARIGAGGETRVLLEAERGADLREA
                     VFRECVARGWTLLELAPRQATLEDVFVRLLATREAEHGPGGEGLPTAVEETGSGEARS
                     "
     gene            12648..13385
                     /locus_tag="PLP50_11820"
     CDS             12648..13385
                     /locus_tag="PLP50_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52278.1"
                     /translation="MKALLAILKREFLAYFFSPLAYVVLTAFLFINGYVFYAILLSLN
                     SPETPRQELMALFFKNVFYWIFMMLVSSIVAMRLVSEERKSGSIEALLTAPVGEGTVI
                     AGKFLGGLTFFLFLWLPTVLYPVALSRYGTIDFGPIAASYLGIALFGALFLAAGTFAS
                     ALTKNQIIAAIVSFVMILALFLAGVFRELVTDPKLREGLGYLNLIEHMDDFARGLVDT
                     RRVVYVVSAVVFFLFLATKALQGNKGK"
     gene            13387..14826
                     /locus_tag="PLP50_11825"
     CDS             13387..14826
                     /locus_tag="PLP50_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPA52279.1"
                     /translation="MSESRTARRVNLGLAILIGLGILVLVNWLGARHYRRFDWTSAGL
                     YTLSPKSTEVMKALTKPVSATVFLTEGSPLYAETQELLKRYQAASPLLTVETLNPERN
                     PARAQAFLREMGDIRLSVVFRSGEKRKVVPVDALADWDFSRAEMGGEPALKGFKGEQE
                     FTAAILSVTQERSPKVVFTTGHGERPLSGRISEGYERFAGLLKQDNCAVEEWDSLADQ
                     KVPEGTDLVVVAGPRAAFTFPETDALQAYLGSGGRALLLLDPEFAPGAANRLVDLGLG
                     PVLAAYGVRLGDDIVVDPRNALPLMGPETVVSRSFRPHPVTKLLQGLPVVFPVVRSVG
                     AVEPAPKDVSVTVLAETSGEGWGETNLADLETRVAKDEKDVAGPVPLAVAAESATEKK
                     ARLVVVGDADFASTGGISNAANLYLVTSAVNWLLERESLISIPPKSTDQVAVSLSRGD
                     VGRITLLVLLVLPLAAIALGLAVWLRRRR"
     gene            14837..>15362
                     /locus_tag="PLP50_11830"
     CDS             14837..>15362
                     /locus_tag="PLP50_11830"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPA52280.1"
                     /translation="MSTRKLLILTGVFFGLLAFVVFWERHQPTSDEAAKARKRLVELD
                     PKAVSRLVLERTDQPKVELAREDGRWRLAGPRGGAADAVTADGLAADLARLDLLGETR
                     TEFDPEAHGLASPRVRVTVTTTDGRARTILFGDPVPGADATAASVDGRLGSVRFAPIA
                     QLTKPYDEYRSKSLL"
BASE COUNT         2076 a         5484 c         5804 g         1998 t
ORIGIN      
        1 cgcgggcgaa cccgtggttc cccgcgccgt cgatcgcctc gaagtggacc gggacgtcgc
       61 cggtgagacc ctgcgggagg aagagctcgg ccgcgttcgc cttcgcggcg ccgtcccacc
      121 ggagcggcac cggggcgccg tcgccgacgc gtgcggtgag cgcgatgacg tcggcgtcgg
      181 cccgggccgc gaggcgcacg cgcgtccccg cggcggccga ggacggcagg accggcgtga
      241 cggtcggggg agtgccgtcg acgatgaccc tcttcgactc gacggccgtc gcgccgttcc
      301 ggtcgcggac gacgatccgg acgtccatcg ggccgtcctt cgttccgtcc gggacgagga
      361 agcgcccctc ccagagcgtc gagcctggcc ggcgcaggag gtccagcctt cggccgaacg
      421 gcaggagcgc ggcgacggcg gccgtccccg gcagggcgtc gatgcggatc accgggtcgc
      481 ccggctggat ccgccgcggc cggaggaggg cgcgcggcgc cgcgaggaag gcggtgtagg
      541 gcgtgacgag cttgtagcgg cgcgagagct cgaggatctc ctcgacccat tcgcgcttct
      601 ctccctcgag ctcgatccgg cgcaggaggt cgtccacccg cgcgcgcgcc cagcggcgcg
      661 gaaggtcgcg cgcgtcgagg gaccgctcgg ggaagcgcgc ctcgacggag gcgccgccga
      721 gctcgatcgt cgccttcgcc ggacgcgcgt agcggccgac ccagcccgag agcgaccccg
      781 cgagcggcgg ctgcgtcatc acgccgtaga cgtcgcgaag ctcggggtct tcgcccttcc
      841 gcgcgaaggg aagcctcgac gcgcttccct cccgcggctc ctccttcggg agcggcgcga
      901 agagctgggc gaggaagagc gcctcgccgg gcggcgcctg gagcgcgctg ccgggcggaa
      961 ggaggaggag ctggcgcgag gagacgacga aggcctccct ccgctcttct ccggtcagaa
     1021 cggtgaagag cggccggcct gccgcggacg cggcgagcgc cccgggcgcc tcgactccat
     1081 cggtcaccag gacgaggcgc gcgtcgctgc cggacggaac gagcgcggcg gcggcgcgga
     1141 gggcggaggc gacgtcggtc ccggggctga gggggcgcgc gcggaggtag gagagcgcgg
     1201 cctccttcgc ggcgggggtg gcggcgacga ggccggccgg gtcgggagcg ctctcgcggt
     1261 cgaacgggac gaggacgaag cggtcctccg gtccgagccg gtcgaggagc cgcgtcagga
     1321 acgcccagcc cgtctcgagg ctcccccagc ggctcgagag ggaggtgtcg aagaggacgg
     1381 cgagccggag cggggcgcgg ccccgcgcgt cggcgacgga ggtgccggag gcggccgccg
     1441 gccggtgctc gaggaggaag aagccgtcct tttcgggcgg gacgtcggcg agccgctccc
     1501 agggggcgag agcgactccg tcgggaaggc ggccgtcggg gctcctgaag gccgagaacg
     1561 tgagcggagc ggaagaggca gggcggaagc ggacgacgac gtccttcgcg aggggaacgg
     1621 cgctcccgcg gaaccgggcg accttgcccg acacggcgag cgggagcgcc gtcgagggcg
     1681 cctcgaggaa cgcgccgtcc tcgagcgtga cgttcacctc gaggctcccc gccacggccg
     1741 gctctccggc acccgggcga agcgggatca ggagctcgcc cacgccgtcg gcccacggga
     1801 cctcctcctg gtattgcagc tcgagccgct tcgttccgta gccgggaatc ggcgcgactt
     1861 tcaccgagaa gacggcaccg cccgagggcc gtcccggtcc gccggagctc gcgtcctcct
     1921 cctcgccctg ctggagcagc cctgggtcga tccggcgtgt cgtgagctcc tcgtagagag
     1981 cgcgcgcccg cttcttctcg acgatgacgc cggggatccg gacgaggccg tcccagacgg
     2041 cgaagtcgcc gacggcgccc gagggcgaga ggcggaagcg ccaggtcccc tcctgcacct
     2101 cgcccgtgtg gttctcgaag acctgccgga cgttcacccg ggcatagccg cgcgcgacgc
     2161 cgacgtcgac ggccatctcc cggagcgtca ggacgcgggc gtcggggcgg cccgtcgagg
     2221 tcggcacgag gaggcccgtc tgggcggcgg ccgggagcgc ggcgaggagg gcgaggagcg
     2281 cggctcgttt catcgtgtct ccggaagggc gtcggccggc agtcggagga ggccctccga
     2341 cgtgccggcg aagaagtgct cgggggcttc cttcgggaac gcggcgagag agaagacgtc
     2401 gggcgaggga agagggaggt cgaggcgcgc gaatcgcgtc cgggcccggt cggagcacca
     2461 gacgccggag ccctgggtgc ccgcgcagag ccggccgtcg gggagcgcga ggagcgcgcc
     2521 ggcgttcacc cggaggcctc gtgtctccgg aaagggctcg aagggggcgt ccgcgccgcc
     2581 ccggcggcgg acggcgatcc cgcctccgta cgtcgcgatg aagaggccgt cgtctcccgc
     2641 gaagagcgcc gtcacccacg gatgcgggag cttcccctcg cccgcgagga cgcgccacgc
     2701 cacgcgctgg cgctcgaccc gtccgaggcc ggtcggcgtt ccgacccaga gcgaggagcc
     2761 gtccgccgcg agggcgtagg cctggttgcc cggcagaccg tgccaggccg agaggagtcg
     2821 gcgcgccggc aggaggactc cctggccgta gccgatcgcg aggccggacg tcgtctgcgc
     2881 gagagcgaac gccgcgcccg cgccgtcgac cgcccggacg tcctcgccct cgatccggaa
     2941 ggcgccgcgg agcgttgccg cccagacgac gccggccacg ggcaggatcg cgttcacgcc
     3001 ccaggcgcca cccgaggggg cgggagaggc ccggagcggt gacgcgcccg ggtcggcggt
     3061 cgcgaggccg ccgtcgaaga agccgagcca gaggcgcgct ccgtcggcgg cgagggcgtt
     3121 gacgtgcggt gcggggaggc cgggcgacgc ctcgcggacg agctcgaagc cgcgcgccgg
     3181 gtcgtggcgc cgaagacccg tcggcccgtc ggcgaggagc tggccgcccg cgacgaagag
     3241 gcgccggaac cgctccggcg cggggacctc ccggcgggtt ccgtcgggaa ggagcgaggc
     3301 gagacgcggc gggtcgtccg tggggacgaa gcggaaggcg ccgtcgaacg cgaccccgtc
     3361 gacggcgtcg gcgccgcgcg ggtgcgccgt cagcgtcgtc gcctcgagag gtccgagcgc
     3421 cgcccccgtc gccgtggcga cccagagctt cccgtcgacg accgccaccc gcgagaccca
     3481 tcggtccggc gtcgcgacga ggcgcgcctc cgcccggccg ggcgcgacga tccggaggcc
     3541 ggactcgcct ccgaccgcga cctctcccgt cgggaggagc gcgagcgaga ggaccgacgc
     3601 gtcgtcgagc ttctcgagct cgcccgaggc gaaggaggcg cggaagagtc cctggcgggc
     3661 gccgaggagc agctcgccgc cgtcggtctc cgcgaaggag cgcacctcca gggggtcgaa
     3721 cccggagacg gccctctccg gtcccgacgg agccagccgg ccccagccgc cgcgctcgag
     3781 ggcgaagacg ggtgtgccgc gccacgacgc gacggcgccg acgcgcaggg aggggaggcc
     3841 gtcgaggacg ccgagagtcc gcccccccgc ctcgagcccc gagccggtgg cgaggaggag
     3901 ctcgccggag gcctcggcca cgccgaggac ggccccgtcc cccgcggcgt cgcccgcgac
     3961 gggggtcggg gacagcgcct cgaatccgag cggccggacg ctctcggcgc gaagcgcctc
     4021 gggcgcggcc gcccgcgtca cggcgcggtc gacccggaaa aggaggagca aggcggccgc
     4081 ggatgcggcg agaaaagctc cgcccgcgag gaggagcgcg cggcgcgcgg caggggacat
     4141 gctgggccga gtctaacggg cgccggagcc ggcgcggggg cgatccgtcc gcctaacgtt
     4201 tgaagaagcc cgactgcggc gtcccctgcg acggagggtt cccgccgccc atgatccggt
     4261 tgttgagctc cttgatccgc tcgttgagct gcgcgatccg ggcggcggcc tcctggcgga
     4321 cccgctcgtt ctcctgacgc gccttctgca gctcgctctc gagctcctgg ctccccatcg
     4381 agttcgcctc gaggacccgg tccttggact gcaggtcggc ctcgaggttc gcgatcaggt
     4441 cgtccttctc ctggaggcgg agcttccact tctcctcggc ttcctcgtag agcttcttga
     4501 tgtccatgag ctcggtgatg gcggagaagt cgttcgcgga cggggccatg attcctcctc
     4561 ggaacttttc ctcgatctgg gggaagagct tgcggagctt caaggagagg tcctcggggt
     4621 ccgcggtgag ctgcatcgcc ttctcgtagt cgatcgcccc gttcacgagg agagcgagaa
     4681 gcgactggtt catcgactgc atccggtagt aggtaaccga cgactccatc tcgtcgagga
     4741 tctcgttgga gtgcccctcc tcgatcagct tcgtgatctt cggggagttc acgaggatct
     4801 cgatgaccgg gacgcgcccc tggccgtcga tccgctcgat gagctgcatg gagcagatcg
     4861 agcggaggac gagcgcgagc tggcggcgga cttggttctg ctggccgccg gggcagctgt
     4921 cgaggatgcg gtcgatcgtc tgcgcggcgt tgttcgtgtg aagcgtggag aagacgaggt
     4981 gcccggtctc ggccgcggtg atcgcggtct gcatcgtctc ccagtcgcgc atctcgccga
     5041 ccatgatgac gtccgggtcc tggcgcatcg cgttgcgcag cgcctcgtgg aaggagaccg
     5101 tgtccgtccc gacctcgcgc tggcagacgg agccgaggtt gtcctggaag aggtactcga
     5161 tcgggtcctc gacggtgacg acgtggaccg ggtgccgctc ggtgatcccc ttgatgatcc
     5221 cggcgagcgt cgtcgacttg cccgagcccg tcggcccggt gacgaggacg aggcccgacg
     5281 gatagtcgca gaaggtctgg atgacgtccg ggaggtgcag ctcctcgacg cccggaatcg
     5341 tgaaagggat ccgccggaag acggcggacc agctcccgcg ctggaggaag atgttgccgc
     5401 ggaagcgggc gagcccctgg atgccgtagc cgatgtcgac ggcgtagttg gccgtcagct
     5461 tctccttctg gtggggcgtg aggatcgtga ggaccatgtc ctcgatctct ttcggctgga
     5521 gcgggtcggt cttcagcggc acgagccgcc cgttgatcct gagaaggggg ggacgccccg
     5581 gcttcaggtg gaggtccgac gcctccttct tcgtcacgaa cttcaacagt tcggtcaggt
     5641 ccatgttcca gatcctcgca cgaagcttat accgtccgtc gcgtccgacg acctcgccgc
     5701 gcgtcggggc ggggagatcc ggagaggaag gcccgcgccg caccggctcc gcggatcgcc
     5761 cccgtcgcga gcgcgtcgtc gaggcgctcg agggcgcgtc cgagctgcgg accggtggca
     5821 cccgtgagga ccgcgaccac ggagaccgga aggggacgcc gcggacggag gagtcggccg
     5881 agcgcgccgg gcggcgcgag gaccgcgtcg acgcagcgcg cgagagcgcg gcccctcggg
     5941 cctcccgtcg cctccacgaa ggcgatggcc tcccgcgaga acggagccgc gcggcggagg
     6001 agccggaccg cctcggtcgc gtcggggcgg gcggacggag cgggaaggcc ggcgagcgtc
     6061 gccgcgagtc tccggtcgtt ccggctcgcg cggagcgcgt cgaggaccgc agccccgcgt
     6121 tccgtcggag cccccgaaag gaggaccgcg cggaggaggg cgggctgcgc gtcgcgccgg
     6181 acggccgcga gcgcggccag cccgcgccgg cgctcctcca cgtcggtgcc ggggaggagc
     6241 gccgcgaggc cgccccacgt ctcgagctgc ccgagcgcgc gcgcggctcg cgcggaaccg
     6301 acggcgagga gcgcgtcgag ctccgcgagg cggcgctcgg gggcgacggc gccgagtccc
     6361 cgcgcgccga cggcgagctc ggtgcgcatc ccggggtcga ggcgaaaccc gggcaggcgc
     6421 gcgaggaacc gggcggcccg gacgacgcgg agggggtcct ccagcaggac tccggggcgc
     6481 ggaagccgaa ggcggcgcgc ggcgaggtcg tcgagggctc cctcgggggc gacgagacgc
     6541 gcgcccggga acgcgagcgc cagggcgttc acggtgaagt cccgccggag gaggtcgtcg
     6601 gcggggtccc cttcggtctc ccagatgtcc acggacgacc ggcgcaccgg cacgtggagg
     6661 atgcgccgtg gctcccggcc gatcggaacg acccgcgtcc cggcgagccg cgcgagcgcg
     6721 gcggcgaagc gcgcggcgcc atcgcgggag accgcgaggt cgaggtctcc ggccgggagg
     6781 ccgaggaggg cgtcgcgcac ggcaccgccg acgacgagga agggtgtccc ggagcgcccg
     6841 gacgccgttc gcgcgagacg gaccgcgctc cggcgcgcga ggcgggccag cgcggcgcgc
     6901 ggctcttgga ggaggctcac ggtcgtcggc gacggtaggg gagcgcgccg ggacgcgtca
     6961 agccgctggc tatactcgaa tccgtgagga tcgacggcgg cctcgtccgc aggatggcgc
     7021 ggctcgccgc gctggacctc gccgaggagg aggcgggccg gctcgccgaa gagctccgcc
     7081 gggtcctcga ccatttcgag gtgatccgct cggtcccgga cgacctcctg ccgcccctct
     7141 ccgacgcccc cgccgcgctg ctccgggacg acgtgccgtc cgcggaggcg ggcgagcgcc
     7201 cgttcgccgt cgcgaatgct ccggagatgg ctcgggggca cttcgtcgca ccgcgcgtcg
     7261 tctcgaggaa cgggtgaccg gagaagcgga ccgccttctc gacggaccgg cgcaccgcct
     7321 cgccgttgcc gtctcgacgc gggaggtccg ggcgcgggac gtcaccgggg cggcgctcgg
     7381 ccgggccgcc aggagccgcg agcggcacgg ggcgttcctt cacgtcgcgg gagaggacgc
     7441 gatcgaggcc gccgccgaag tcgaccgtcg gatcgcggcg ggagagacgt tcccgctcgc
     7501 cggagtcccg atcgccgtca aggacaacgt ccacgtggcc ggcatgccga cgacggccgg
     7561 ttcccgcgtc ctcggcgcga tcgtcccgcc ctgcgacgcg acctgcgtcg cccagctacg
     7621 cgccgcgggg tgcgtggtcg tcggcaagac gagctgcgac gagctcggga tgggctcctc
     7681 gaacgagacg tcggcgttcg gtccggttcg gaacccctgg aatccggacc gcgtccccgg
     7741 cggctcgtcg ggcggctcgg ccgccgccgt cgcggcgcgc gcggtccctc tcgcggtggg
     7801 gaccgacacc ggcggctcga tccggcttcc ggcggcgttc tgcggtctcg tcggcctgcg
     7861 cccgacgtac ggccgcgtct cccgctctgg cctcgtcgcc tacgcctcct cgttcgacca
     7921 ggcggggccg atcgcccggg acgtgaggga cgcggctctc gcgctcgcgg cgatggcggg
     7981 cgccgacccg tccgacgcca cctccctccc cgggcctccg cccgacctcc tctcccggct
     8041 cgacgccggc gtcgcgggcc tgcgcgtcgg ccttctcgac cacgcggggg agggagtcga
     8101 ccccgacacg gaggcggccc tcgcgtcgac ggcgggcctc ctcgcgtccc gcggcgcgtc
     8161 ggtcgtcccg ctccgcctcc cgcgcctctc gctcggcatc gcggcgtact acctgatctc
     8221 ggccgcggag gcctcctcca acctggcgcg gttcgacggc gtccgcttcg gcgcccgcgt
     8281 cgcgggcgac ggtccgggcg ctctcgtccg cgcgacgcgc gcggccggct tcggcgacga
     8341 ggtgaagcgg cggatcctcc tcggtacgtt cgccctctcc gcaggccacc gcgaagcgtg
     8401 gtacgagcgg gcgcagaggg tccgggcccg gatcgcgctc gacctcgccg acgccttccg
     8461 cgcctgcgac ctcctcctct cgccgaccgc gcccggcccg gcgttcccga tcggcgagaa
     8521 gagcggcgac ccgctctcgc tctacctcac cgacgtcttc accgtccccg cgagcctcgc
     8581 ggggcttccc gccctgtcgg tcccggcggg gctctcgcga gagcagctcc ccgtcggggt
     8641 ccagctcgcg gggccggcgg gatcggaggc gctcctgctc ggcgtggcgc gggagctgga
     8701 ggcggcggcc ggcccccggc cggacccgcc gggcgtgatt cggtgagagg ggctctctcg
     8761 agggcggccc tggcggccct cctgctcgtc ccgttcctgg ggcaggccca ggcggcgctg
     8821 cgccgggtgg aggtctccga ggacttctcg gcgtacctcg accagggctc gatcctcgtc
     8881 gaggtgcgcc cgctcgacgg cgagtcggtg acggacttcg cccgtcgcat ctcccgcgac
     8941 gaggccgcgg ccgcgcggct cctcgccctt ccggccgccc cgtcccggag ccgccggatc
     9001 gtcctgccgt acggcgacct ctccgacgag acgcgcctcg ccgccgtccg cgccctcttc
     9061 cccgcggacg tccgcgcgac gatcggctgg ctccacgtgg ccgtcgagac ggagccgctc
     9121 gcgtcgatcg cgacgtggtt cacgggcgat gcccgcctcg cctcccgcct cgcggagctg
     9181 aacggactct cgggaagcga gccggtgacg cgcgggacgc tcgtgaagat cccggtcgag
     9241 tacctgcttc cgccgttccg ggacgcggag ccgatccccg acgaggagcc tccccgcctc
     9301 gagtacggct ccgacgagaa ggggcggtac gccgtctatc gtctcaagcg gaaggaggcc
     9361 ctctactcgg cggtcgtcgt ccgcttcacg ggccgcatcc acgccgagga cgtcatccag
     9421 ctcgcgctcg acatcgccct ccggtcgggg atcgacgacg tccacgcgat cccggtcggc
     9481 taccccgtga agatcccgct cgagctcctg tccgaggagt acctcccgcc cgacgacccg
     9541 aaggccgccg cgcaggcgaa ggagcgtgcc gaggcggccc tcttcgcgac accggcgaag
     9601 gcgctcttcc tcgcgggggt ccgggtcgtg atcgacgccg gccacggggg gaccgacacc
     9661 gggacgcttc accacggcgt ctgggagtcg acctacgtct acgacgtcgc gtcccgcctg
     9721 cggaaggtcc tcctcgagac gacccgggcc gaggtcgtca tgatgacgcg cgagcccggg
     9781 ctcggctggg aggtcctcga cagggaccgc attccagacc ggaaggggcg tgtcctgctg
     9841 acggacccgc cgtacaagat gacgaacgtc gtggcgggcg tgaacctccg ctggtacctc
     9901 gcgaacgcga tcctgaagcg gccgggtccg ggagggaagc cgatcccgcc ggagaagacg
     9961 gtcttcctct cgctccacgc cgattcgctc cacccgtccg tccggggcgc catggcgtac
    10021 gtccccggcg agcgtttcct gcgcgatcgc tacggccggg cgacggagtt ctaccggagg
    10081 ttccgcgagg tgcgggacga cccggtcgtc tcgttcacga agaaggagcg cgtcctcgcc
    10141 gaaggggcct cgacctccct cgccgagaag ctgatcggcg ccttccgccg tcggagtctc
    10201 ccggtccacc agttccgacc ggtgcgaacg cacgtcatcc gaaaggggcg ggagtgggtc
    10261 ccggcggtcc tccgctacaa caagatcccg aaccgggttc tcttcgagct ctcgaacctc
    10321 tcgaacgagg aggaccggag gctgacgctg acccgcgcgt accggcagga gtgcgccgag
    10381 gcgctcgcgg acggcctcgt cgcgttcttc ggcggggcgg cggacgagcg gggcgcgtcg
    10441 ccgtccacgg cggccgggcg acaggcgccg cgcgtcgccg ccgcgcccgc gccggcggag
    10501 ctcccgggcc cctggcccga cacgatcggc ccctggccgg aaggggcgcc ccaggggcgg
    10561 accgcgctcg tgccgagccc gaccccgacg ccgacgaaga ccccgcggaa gcccgtccgg
    10621 cgacgccggc gttgaggcct caggccttgt ccggcgtctc tctcgacgtc ccgaggcggg
    10681 cgagggcgag acccccgaga acgacggccg cggagacgac ctttcccccg gagaagcgct
    10741 cgccggtcca gagggccgac gcgacccctg ccacgagcgg gacgaggacg ccgatgcggg
    10801 tcgtggcggc gacaccgagg accgccagcc cgtgggccca gaggcggaac gagacgtaga
    10861 gcgggaagac ggcggagagg acgaggatga cccaggcggc cgtggagacg gaggagtagt
    10921 cctgccggag catcgccggg agacagtagg cgagcaggac ggggacgccg aacgtgaagg
    10981 tcgacgcgag cgccgtcgcg tgggggatgc gtgtcgagta gcgggaggag aggacgctgt
    11041 agaccgacca gcaggtggcc gagacgaggc agaggaggtt gcccgtcagg ccgccgagat
    11101 ccgctgaccc gtgggccgag gagaggaacg cgaggacccc gccccaggcc acgacggtcc
    11161 cgaccacggc cgtccgcggg atcttctcct gcccgaggag ggcgaccagg aggagggaga
    11221 ggagcgggga gacggagttc aggagggcgg ccgcgaagcc gctcgtcttc gccaccccga
    11281 tcgcgaagaa gagctggtag acgccgacgc cggtcatggc gagcaggaac cccgggaagc
    11341 ggaggccgcg gggcgggagg acgggggcgt ggttcctccg ggcccacgcc aggatgaggg
    11401 cggccgccag gaggaagcgc cagccggtgt aggcgagggg ggagagctcg cggtaggcga
    11461 tcttcgtggc ggggtagttc gtcccccaga cgaggacgca caggaagacg ccgaggaggg
    11521 cccgggaacg ggcggcgggg gagggctgca cgtgcgattc gctctgctat atttcccgcg
    11581 ctttcgtggc ggagggcggc gcggacggcg ccgccctctg cccgtccacg gagggactgc
    11641 acgacgatgc ttgaggttga agcgctccgc aagtcgttct acgggacgct cgccgtggac
    11701 gacgtctcgt tccgggtcga gagcggcgag atcctcggct tcctcggccc gaacggcgcg
    11761 ggcaagacga cgacgatgcg gatgatcacc gggttcctcg agccgaacgc gggccgggtg
    11821 acggtcgacg gggtcgacgc ggcgaagaag ccgcgggagg cacgcgcgcg gatcggctat
    11881 ctccccgaga cgctcgccct ctacccggag atgcgggtga gggagtacgt gagattccgc
    11941 gccgagctct gcggcgtccc gcgccgcgag acgaaggccc gcgtcgacga cgcgctcgcg
    12001 acctgcttcg tcgacgacgt cgcgtcgaag ccgatcggga acctctcgaa ggggtatcgc
    12061 cagcgcgtcg gcctcgccgc cgcgctcgtg cacgcccccc aggtcctcgt cctcgacgag
    12121 ccgaccgtgg gcctcgaccc gcgccagatc gtgaaggtcc gcgacctgat ccgcgacctg
    12181 aagaaggacc acacgatcct cctgtcgacg cacatcctcc cggaggtcga gctcgtctgc
    12241 gatcgcgtcg tcatcatcga ccgcgggaag atcgtcgcga cggggactcc ggagacgctc
    12301 cgccagcagc tgacgcaggc gcccggcctc gccgtcgcga tccaggggga ggtccccgac
    12361 gccgacggga tcctccggaa gctcccgggg gtcgtccgtg cggcgcggat cggcgccggc
    12421 ggggagacgc gggtcctgct cgaggcggag cgcggagccg acctgcgcga ggccgtcttc
    12481 cgcgagtgcg tcgcccgcgg ctggacgctc ctcgagctcg cgccccggca ggcgaccctc
    12541 gaggacgtct tcgtgaggct cctggcgacg cgggaggcgg agcacggacc gggcggagaa
    12601 ggtctcccga cggcggtcga ggagacggga agcggggagg cgcggtcgtg aaggcgctcc
    12661 tcgcgatcct gaagcgggag ttcctcgcgt acttcttctc gccgctcgcc tacgtcgtcc
    12721 tgacggcgtt cctcttcatc aacggctacg tcttctacgc gatcctcctg tcgctgaaca
    12781 gcccggagac cccgcgtcag gagctgatgg cgctcttctt caagaacgtc ttctactgga
    12841 tcttcatgat gctcgtctcc tcgatcgtgg cgatgcgcct cgtctccgag gagcggaagt
    12901 cgggctcgat cgaggcgctc ctgaccgccc ccgtcggcga gggcaccgtg atcgccggga
    12961 agttcctcgg gggcctgacg ttcttcctct tcctctggct tccgaccgtc ctctacccgg
    13021 tggcgctgtc gcggtacggc acgatcgact tcggcccgat cgccgcgagc tacctcggaa
    13081 tcgcgctctt cggggcgctc ttcctcgcgg ccgggacgtt cgcctcggcg ctgacgaaga
    13141 accagatcat cgcggcgatc gtcagcttcg tgatgatcct cgcgctcttc ctcgcggggg
    13201 tcttccgcga gctcgtcacc gacccgaagc tccgcgaggg gctcggctac ctgaacctga
    13261 tcgagcacat ggacgacttc gcgcgcggcc tcgtcgacac gcggcgcgtc gtctacgtcg
    13321 tctcggcggt cgtcttcttc ctcttcctcg ccacgaaggc cctgcagggc aacaagggga
    13381 agtgagatga gcgaaagccg cacggcccgc cgcgtcaacc tcggcctcgc gatcctcatc
    13441 ggcctcggga tcctggtcct cgtgaactgg ctcggcgcgc gccactacag gcgcttcgac
    13501 tggacgagcg ccggcctcta caccctttcc ccgaagtcga cggaggtgat gaaggcgctg
    13561 acgaagccgg tctccgcgac cgtcttcctc accgagggct cgccgctcta cgccgagacc
    13621 caggagctcc tcaagcgcta ccaggcggcc tcgccgctcc tcacggtcga gacgctgaac
    13681 ccggagcgga accccgcccg ggcgcaggcc ttcctgagag agatgggcga catccgcctc
    13741 tcggtcgtct tccggtcggg ggagaagcgg aaggtcgtcc cggtcgacgc gctcgcggac
    13801 tgggacttct cccgcgccga gatggggggc gagcccgcgc tcaaggggtt caagggagag
    13861 caggagttca ccgccgcgat cctctccgtg acgcaggagc ggagcccgaa ggtcgtcttc
    13921 acgaccgggc acggcgagcg gccgctctcg gggcgcatct ccgagggcta cgagcgcttc
    13981 gccggtctcc tgaagcagga caactgcgcc gtcgaggagt gggacagcct cgccgaccag
    14041 aaggtccccg aggggaccga cctcgtcgtc gtggccgggc cgcgcgcggc gttcaccttc
    14101 cccgaaacgg acgcgctgca ggcgtacctc gggagcgggg gccgcgcgct tctcctcctc
    14161 gacccggagt tcgcccccgg agccgcgaac cgcctcgtcg acctcggtct cgggccggtc
    14221 ctcgcggcgt acggcgtccg cctcggggac gacatcgtcg tcgacccgcg gaacgccctg
    14281 ccgctgatgg ggccggagac ggtcgtctcg cgcagcttcc gccctcaccc ggtcacgaag
    14341 ctgctccagg gcctcccggt cgtcttcccg gtcgtccggt cggtcggcgc cgtggagccg
    14401 gctccgaagg acgtctcggt cacggtcctc gccgagacct cgggcgaggg gtggggagag
    14461 acgaacctgg ccgacctcga gacgcgggtc gcgaaggacg agaaggacgt cgccgggccg
    14521 gtgccgctcg ccgtcgcggc cgagtcggcg acggagaaga aggcccggct cgtcgtcgtc
    14581 ggggacgccg acttcgcctc cacgggcggg atctcgaacg ccgcgaacct ctacctcgtc
    14641 acgtccgccg tgaactggct cctcgagcgc gagtccctca tctcgatccc gccgaagtcg
    14701 accgaccagg tcgcggtctc cctctcgcgc ggcgacgtgg gtcggatcac cctcctcgtc
    14761 ctcctggtcc tcccgctcgc cgcgatcgcg ctcgggctcg cggtgtggct ccggaggagg
    14821 cgctgacgga gccgccatgt cgacccggaa gctcctgatc ctcaccggcg tcttcttcgg
    14881 cctcctggcg ttcgtcgtct tctgggagcg tcaccagccg acctccgacg aggcggcgaa
    14941 ggcgcggaag cggctcgtcg agctcgaccc gaaggcggtc tcgcggctcg tcctcgagcg
    15001 gaccgaccag ccgaaggtgg agctcgcccg cgaggacggg cggtggcggc tcgcgggccc
    15061 ccggggcggc gccgcggacg ccgtcaccgc cgacggtctc gccgccgacc tcgcccggct
    15121 cgacctgctg ggcgagacga ggacggagtt cgacccggag gcgcacggcc tcgcctcgcc
    15181 ccgtgtccgc gtcacggtca cgaccacgga cggccgggcg cggacgatcc tcttcggcga
    15241 tccggtcccg ggggccgacg cgacggcggc gtccgtggac ggccggctcg ggtcggtgcg
    15301 gttcgctccg atcgcgcagc tgacgaagcc gtacgacgag taccgctcga agagcctcct
    15361 cg
//
LOCUS       DAOONL010000080        15198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4169_length_15198_cov_16.754672, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000080 DAOONL010000000
VERSION     DAOONL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4169_length_15198_cov_16.754672"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLP50_11835"
     CDS             <1..431
                     /locus_tag="PLP50_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPA52281.1"
                     /translation="RERFDAALARARALLGAGARGPDPSLLAAAARPEGEAIGRVAVR
                     EGTRVTVLPVEAIDWIGAEGDYVRIGSAGGSHLKLQALADLANRLPADRFVRVHRSWV
                     VNVARLAALEEGRTAVMADGTRVPVSRAGAARLKELLPKG"
     gene            complement(448..1146)
                     /locus_tag="PLP50_11840"
     CDS             complement(448..1146)
                     /locus_tag="PLP50_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52282.1"
                     /translation="MRTDVFVIATALAALSTIPAQAQWGGSRKGEGALVLRDGAPAYR
                     GKGSSDVKRTFSKGEAVAGLHKEFLAMTYELFEEDGRVRVLYPKPGSALMEDAWMDPA
                     DLLPFAYDCCDEDCTPVKGSFKRAEWNACFREGLERARARFAARERGGAGQRRAGEAR
                     KPASSGPKEKPLTNDDVLAMVKADLGDELVIGKIRQAPREELDVSPETLVKLKQEGLS
                     RPVLDAMIRRAGER"
     gene            complement(1210..3210)
                     /locus_tag="PLP50_11845"
     CDS             complement(1210..3210)
                     /locus_tag="PLP50_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA52283.1"
                     /translation="MTLTRTFRVSGLAFLAGVLLTALGGDAGFAEALRVVGILLLVVS
                     VPVFGVSLLRHLLRGLLWRVGSRLFVSYLLIGVLPLFLVAGLAAAALFILAGQTGARR
                     AEARLVARLDALEAKASEFGSREKARRPELVRGSGAFDGWALLPASGSPEGDGPLGTA
                     LEKVAVPETGLRALLRSDDRSFLAVGRRTKGGALFLYRTADATLEDELSTEAGLNVRF
                     GLGKTEATGQAGATQAEGTGGSLTIESPGQRTRVASVNQEERKAQGARAPGPGDGGWV
                     VWFLALDLPVLDAATGVRLKDEGGALLLRTSFTAEFRALFGEAKLSDSGAASGTIALA
                     LLKGLATATLVVYLVAFGVAALLVARIARAARRLSHGFAEIEKGNFAVRERLRGRDQL
                     AGLVSGFNEMAGHLEASVAARAEAEALGRELALARDLQRRLLPAPDFAFPGVELAADF
                     RPAAAIGGDFYHFLGEGESALTVVIADVAGHGLPAGIVMASALASLTALSRGGADTPT
                     LLGLLDDEVRRTTERRAFVTLAHVRLDLAARRAAFTNAGHLYPYRVDPSGAVSWVENP
                     AKPLGVGRPAERITVSVPLVDGEVWVLLSDGVVEAMASDGSEAFGFDRLETTLAACGG
                     KSAREVLDAVLAAWRAHTGGDDPEDDRTVVVVRMLARGAAAG"
     gene            3314..4765
                     /gene="cls"
                     /locus_tag="PLP50_11850"
     CDS             3314..4765
                     /gene="cls"
                     /locus_tag="PLP50_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPA52284.1"
                     /translation="MSLTQILALLIVVADVLALVHAVTRHLGVTSTLAWIFFILFLPG
                     IGPLAYFLLASPRIRTTRRRKRLAGEAVRAAIRRNLGREPLPAEIDHAAPLARSVLYL
                     GTRLTGLPPRAGNRVELLLENEGAFRSKAEAIRGAARSVWAEYYIVEDDATGRAFLAD
                     LAERALAGCDVRLLYDAVGSSDIPEEGLEALCAAGGRTAAFLPLNPLRRRWSAHLRNH
                     RKLLVVDEEIAFTGGMNVGDEYARGSRLAGGSGGPLRNRPWRDAHLRVEGPAVFDLAT
                     VFAEDWSFATGERLVPGRIPMPTADGSVVAVLPSGPDQTANANAHVYFSALSGALERC
                     WLTTPYFVPDEPAARALANAALRGIDVRVLVPAESDVPLARAAGRFFYGSLLRAGVRI
                     FEYVPAVLHAKTVVVDGSWALVGSANFDFRSFALNFELGAVVYDRAFAALLEERFIRD
                     LAESREVTLAAVERRGFAERARLGLARLAAPLL"
     gene            4779..6713
                     /locus_tag="PLP50_11855"
     CDS             4779..6713
                     /locus_tag="PLP50_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPA52285.1"
                     /translation="MRLRSAPSALLALLLAATPLAAADLPIHEIQGAGASSPRVGQSV
                     TTTGVVTALRTNGFYLQAPDAEADADPATSEGVLVFTSSAPPAAAAVGNRVRVSGSVV
                     EYRPSADPASPSLTELGGSVSVALLSTGNALPAPVLLTTADLSPAGPADRLERYEGMR
                     VRVPQLLVVAPTGGSVNEEAATGRSNGVLVGVLPGTPRPFREAGADPLTPLPAGSPCC
                     VPRFDGNPERLRVDTDGLVGVAPLDVASGTTLHDVVGPLDYAFRTWTLLPESAPVATG
                     SPAARPVPVPSSDELTVGSLNLERFFDAEDDGEGPTLNAAAFSMRLAKASLAVRALLR
                     TPDVLAVVEVEDLSTLRALAARLSADALAAGAPDPAYQAFLEGGNDPGGIDVGFLVAS
                     RVSVLSVVQEGKAATYTDPTTGNAATLNDRPPLVLTARWAAPDGTDLVFTVVANHLRS
                     LNDSELDSTTGRRVRAKRQAQAEYLAALLARLQAEGGDAPLLAVGDFNAFEVSDGLVD
                     VIGTVRGAPAPASEVVVNRSVDLVAPDLVDLCSVDTLVPPSERYSYVFDGNAQLLDHA
                     LASGATLPLVRGVAFARLGADFPETMRGDASRPERLSDHDGLVVRIGLAAPKGRSPLV
                     PVRDGEPPSPFRSRTPETAP"
     gene            6710..8380
                     /locus_tag="PLP50_11860"
     CDS             6710..8380
                     /locus_tag="PLP50_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52286.1"
                     /translation="MSRDPLARLEEARRSRPGARAAEVALESAAVFVPRDATELARLH
                     DAALFLRVYPQSPRVLALAEALLARVPAHAARLAGEGADLAPLDDPSVAGIAGVPVAM
                     PFSHAAASWLARRDPSALRVDWDAEDLPDRLAAFLPRFVPFLEEQARVDANVAFRDWV
                     DAARGARPDAPWLLERLAAFPLPDSVRAELWEALSLQLVWTPRAADSRGLARVPSPAP
                     FCSDTPLLSRRDVSVATELAGPPPRLVRLSRREGAALLDAARAAMAIRYRELPAFSAG
                     DPSAVFRFDVGRGVSIFVTGLARDARLPLRAAFGTLFARNGVLVGYGDLHASLGRADV
                     SFNVFYAFRDGESAWLYARLLAVARAVTHAAQLSVDPYQVGLGNEEAIASGAFWFYRK
                     LGFRSADPALERLARREEARLAAAPSRRTPAATLRRLAVAPLLLDVPGETPLLPDSFR
                     LADLGRAAARRFAASKLSPEAFRARAAGRAARALGLDPVAPACRASLEALAPALALLD
                     DLASWPLSDRRALVALLRAKAAPDELRYARLSSGHRRLAASIARAVSG"
     gene            8500..9123
                     /locus_tag="PLP50_11865"
     CDS             8500..9123
                     /locus_tag="PLP50_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52287.1"
                     /translation="MTTRRLIPLVLLVAFAAAAGDARAQDRTAAQREVDRILAGKGTA
                     RGEAACDLLRSGVLANAFGAAAASASYRPGSRYVPNVLCTASWAGAKGTAAGSPPGHE
                     VSLTILDRSFPSRAAAVGSLESSVAELSKGVTVEVKGRKHTSRVEFEGWLEGVGDKAA
                     WAPKPRELSVAYGGVRFAVAVRGFGDAAEEKARAIDLARRVATSLGR"
     gene            9120..12137
                     /locus_tag="PLP50_11870"
     CDS             9120..12137
                     /locus_tag="PLP50_11870"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA52288.1"
                     /translation="MIPAAVSRPGPAGREAARRRGALLAALAALLALPSARDARAQAS
                     AAPAVEIVGPGRLSEGRLHQWSVFMPRRHLSEGPDGYTPNGLDAPADGAPLPTTWERP
                     VAFHWDFGDGTRVVTGERATAGHRYEDDGAFTITVRAVDTAGREVVGTKPVTVTNRPP
                     GRVELFAVEVDPAESLFELSAETSDVAGDPLTIEWDFGDGEKAKGPLPERWKVRHAFP
                     LPGRYPVTVKVEDGDGATREATTHVVSRGGSAQEETSVDALEDGGSTTRNTVSRFDAT
                     ISGSLAASLTGEVRSLAGLHLSPVDGGRRCRFLLTAWDPTHLASVAVILDLPGLPEGK
                     GARYTFRPGRVDLNLDPTAERYAFTRRGLLQGFADSPLAERIVPGVDERLGLDPAPPA
                     AAPPAGDPRPAPAVSPFGLEDHEGFRASGGTLELTFVPYDRATARFDLTLTNLDRKSP
                     YPGLSFKGSFALDLAAARRDGLMNYDQCAPARLEVEEVRPEPGTRHSDGENQWVTARF
                     SGDIDPATLNEETFQLTWPDSSGALVPVEARVHRRGSQALLVPTRPLRAGVRHTARVK
                     TGPAGVRSRGGAALEDTDRSGWHAWDFTTRVDVVPKPGAGGNLSCHVFQTVRDAPLVA
                     GKPAVARIYADWTEHPEVDPGAQVRDFTARVVLREVVGTTSREVASELHRFVRPTLHD
                     SFGVSRRKAEHTANVFFTPESSTPASLVVGLEVPQAPSESHAEAYQTRCATPMWEHAP
                     TLKVRYYLLAVNEWTGEESRARFLPVATDILAEAERWAEQLFPFRSVQFEYGGALSIP
                     PRVKTIPVYHEPGDPPPPPICDVACATEVLRLETARAPADVVIGLMPVQGAYKGGGAL
                     TKVGGQEAPALIRLTLDDVPKHRDRWVFAVVHEIGHTLGLEHLPYLGGAQEDASYERD
                     VLEALRDHSWANAGKPIHWYEGIEGFRIERDGRRGYNKSSVEGNAEGSWLVPLMFPTS
                     MLYRDAFIVRHQYFDLLERIGAWKGPASAAEAP"
     gene            complement(12194..13324)
                     /locus_tag="PLP50_11875"
     CDS             complement(12194..13324)
                     /locus_tag="PLP50_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52289.1"
                     /translation="MTNAVTIREVATARERRAFVRLPFELHRGHATWVPPLISEEKAM
                     LDPRRNPALATCDTTFALAFRGEKPCGRIVGIVSRREVAARGTRVARFGWLECPEDPE
                     VARALLEWVERWAKGLGMVRLVGPMGFSDQDPEGLWIEGFEHEPTIATYANHPWLGRL
                     VEACGFGKEIDFVVYKAAIGPTLPPFYEKVAARVGARSEYRILEFRSRKELAPWAATI
                     IRLMNETYAELYGYVPLDEAEIEALARKYLPLLDPRFVKVGMKGETPIGFLVAMPNLD
                     PGLRKAKGRLFPIGFVHVLRAARASRQLDLLLGGIRAEDRGKGADILGLAAVIRSAIA
                     AGFTSFDSHVELETNLRVRAEMERLGATVCKRYRIYGKPLAG"
     gene            complement(13413..13823)
                     /gene="rnk"
                     /locus_tag="PLP50_11880"
     CDS             complement(13413..13823)
                     /gene="rnk"
                     /locus_tag="PLP50_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPA52290.1"
                     /translation="MNDRNIYVTSDDRKRLQALLSRPTDAMDRDDVADLVAEVQRAVV
                     VPASEIPPDVITMNSRARLLDLDQRTTLELTVVYPEDADFAAGRISVVAPIGAAMLGY
                     RVGDEIEWVVPSGPRRLRVEAILYQPEAAGDFSR"
     gene            complement(14322..14397)
                     /locus_tag="PLP50_11885"
     tRNA            complement(14322..14397)
                     /locus_tag="PLP50_11885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14361..14363),aa:Glu,seq:ctc)
     gene            complement(14508..>15198)
                     /locus_tag="PLP50_11890"
     CDS             complement(14508..>15198)
                     /locus_tag="PLP50_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52291.1"
                     /translation="TGILFFLIYMSIFVYGAQVMRGVLEEKSNRIVEVIISSVKPTEL
                     MLGKVVGIGLVGLTQYAVWALFAFTLTLPGIVGFVGLASGSLPSVPVGTLVAFIVFFL
                     LGYFLYAGLYAALAAPFNSEQEAQQLVMIPGMMLILSAMTWIFAYNTPDGTFSRVLSL
                     FPFTSPLLMFMRISVQPPPAWEIALSIGLLLLTIWGVSWFAGRVYRVGILMYGKKPTF
                     PEIIRWVRAAD"
BASE COUNT         1941 a         5906 c         5290 g         2061 t
ORIGIN      
        1 cgcgcgagcg cttcgacgcg gcgctcgcgc gggcccgggc gctcctcggc gccggggcgc
       61 gcggccccga cccgtccctc ctcgcggcgg cggcgcgtcc cgagggagag gcgatcgggc
      121 gggtcgcggt gcgagagggg acgcgcgtga cggtcctccc ggtcgaggcg atcgactgga
      181 tcggcgcaga aggggactac gtgcggatcg gctccgccgg aggaagccac ctgaagctcc
      241 aggcgctcgc cgatctcgcg aaccgactcc cggccgaccg attcgtccgc gtccatcgct
      301 cgtgggtcgt gaacgtcgcc cgcctcgcgg cgctcgagga ggggcgcacg gcggtgatgg
      361 ccgacggcac gcgcgttccg gtgagccggg cgggcgcggc ccgcctgaag gagctcctgc
      421 cgaagggctg aagccgcggc gggcggctca gcgctcgccg gcgcgccgga tcatcgcgtc
      481 gaggacgggc cgcgagagcc cctcctgctt cagcttcacg agcgtttccg gcgagacgtc
      541 gagctcctcc cggggcgcct gccggatctt cccgatgacg agctcgtcgc cgaggtccgc
      601 cttcaccatc gcgaggacgt cgtcgttcgt gagcggcttc tccttcggcc ctgaggaggc
      661 gggcttcctc gcctccccgg cacgccgctg cccggcgccg ccgcgctccc gggcggcgaa
      721 ccgcgcccgg gcgcgctcga gcccttcccg gaagcacgcg ttccactcgg cgcgcttgaa
      781 ggagcccttc accggcgtgc agtcctcgtc gcagcagtcg taggcgaacg gcaggaggtc
      841 tgccgggtcc atccaggcgt cttccatgag cgcggagccc ggtttcgggt agagaacgcg
      901 gacccgcccg tcctcctcga agagctcgta ggtcatcgcg aggaactcct tgtggagccc
      961 cgcgacggcc tctcccttcg agaacgtcct cttcacgtcg gagcttccct tgccccggta
     1021 ggccggagca ccgtcgcgca ggacgagggc cccttctccc tttcgcgagc cgccccactg
     1081 ggcttgggcc gggatggtcg aaagcgccgc cagtgccgtt gcgatcacga agacgtccgt
     1141 tctcatcgcg ccgccctccc ttccgaggtc ccatcgggca tggtacccct cggaaggtcc
     1201 tcgggccgct cagcccgccg cggctcctcg cgcgagcatc cggacgacga cgacggtccg
     1261 gtcgtcctcg gggtcgtcgc cgccggtgtg ggcccgccac gcggcgagga cggcgtcgag
     1321 gacctcacgg gcgctcttac cgccgcaggc ggcgagcgtc gtctcgagcc ggtcgaagcc
     1381 gaaggcttcg ctcccgtcgg aggccatcgc ctcgacgacg ccgtcggaga ggaggaccca
     1441 gacctcgccg tcgacgagcg ggacggagac ggtgatccgt tcggccgggc gcccgacgcc
     1501 gagcggcttc gccgggttct ccacccagga gaccgcgccg ctcggatcca cgcgatacgg
     1561 gtagagatgc ccggcgttcg tgaacgcggc acgccgggcg gcgaggtcga gccgcacgtg
     1621 ggcgagggtg acgaacgcgc gccgctccgt cgtccgcctc acctcgtcgt cgaggaggcc
     1681 gaggagcgtc ggcgtgtcgg cccccccgcg ggagagcgcc gtgaggctcg ccagggcgga
     1741 ggccatgacg atcccggccg ggaggccgtg cccggcgacg tcggcgatga cgaccgtcag
     1801 cgcgctctcc ccctcgccga ggaagtggta gaagtccccc ccgatcgcgg cggcgggccg
     1861 gaagtcggcg gcgagctcca ccccggggaa ggcgaagtcg ggcgcgggga ggagcctccg
     1921 ctggaggtca cgcgcgaggg cgagctcgcg gccgagcgcc tcggcctcgg cgcgcgcggc
     1981 gacgctcgcc tcgaggtggc cggccatctc gttgaagccg gagacgagac ccgcgagctg
     2041 gtcgcgtccg cgcagccgct cccggacggc gaagttgccc ttctcgatct cggcgaagcc
     2101 gtgcgagagg cgccgcgcgg cgcgcgcgat gcgcgcgacg agcagggccg ccacgccgaa
     2161 cgcgacgagg tagacgacga gcgtcgccgt ggcgagcccc ttgaggaggg cgagcgcgat
     2221 cgtcccggac gccgcgccgg agtcggacag cttcgcctcg ccgaagaggg cccggaactc
     2281 ggcggtgaag gaggtccgga ggaggagcgc gcccccttcg tccttcagcc gaacgccggt
     2341 cgcggcgtcg aggacgggga ggtcgagcgc gaggaaccag acgacccacc ctccgtcgcc
     2401 cgggcccgga gcccgggcgc cctgcgcctt ccgttcctcc tggttgacgg aggcgacgcg
     2461 ggtccgctgc cccggcgact cgatcgtgag gctccctccc gtcccctcgg cctgcgtggc
     2521 gcccgcctgg ccggtcgcct cggtcttgcc gaggccgaag cggacgttca gccccgcctc
     2581 cgtcgagagc tcgtcctcga gggtggcgtc cgccgtccgg tagaggaaga gcgccccccc
     2641 cttcgtccgc cggccgacgg cgaggaagga gcggtcgtcg ctccggagga gcgcgcgcag
     2701 gcccgtctcc gggacggcga ccttctcgag ggcggtcccg agggggccgt ctccttcggg
     2761 ggagcccgag gccggcagga gcgcccagcc gtcgaacgcg ccgctgccgc ggacgagctc
     2821 gggccggcgc gccttctcgc gggagccgaa ctccgaggcc ttcgcctcga gcgcgtcgag
     2881 ccgggcgacg aggcgcgcct cggcgcgccg cgcgcccgtc tggccggcga ggatgaagag
     2941 ggccgcggcg gcgaggccgg cgacgaggaa gagcggcagg accccgatca ggaggtacga
     3001 gacgaagagg cggctcccga cgcgccagag gaggccgcgc agcaggtggc ggaggaggct
     3061 gacgccgaag acggggaccg agacgacgag gaggaggatg ccgacgacgc gcagcgcttc
     3121 ggcgaagccg gcatcaccgc cgagagcggt gaggaggacc ccggcgagga atgcgaggcc
     3181 cgagacccgg aacgttcgtg tgagggtcaa cgacgtactc ccgcggcgcg cggccgcctc
     3241 gaatggtccg gacgagatta cctcgccgcc gtgcgcgctc gcgccggcct aagatgcccc
     3301 gggacgccgc gccgtgagcc tgacccagat cctcgccctt ctcatcgtcg tggccgacgt
     3361 gctcgcgctc gtccacgcgg tgacgcgcca tctcggcgtc acgtcgacgc tcgcctggat
     3421 cttcttcatc ctcttcctcc cggggatcgg gccgctcgcc tacttcctcc tcgcgagccc
     3481 gcgcatccgg acgacgcgtc ggcgcaagcg gctggcgggg gaggcggtcc gcgcggcgat
     3541 ccgcaggaac ctggggcgcg agccgctccc ggcggagatc gaccacgccg cgccgctcgc
     3601 ccgctccgtc ctctacctcg gcacccgcct gaccgggctc ccgccccgcg ccggcaaccg
     3661 cgtcgagctc ctcctcgaga acgaaggcgc gttccgatcg aaggcagagg cgatccgcgg
     3721 cgcggcccgc tcggtctggg ccgagtacta catcgtcgag gacgacgcga ccggtcgggc
     3781 cttcctcgcc gacctcgccg agcgggcgct cgccgggtgc gacgtgcggc tcctctacga
     3841 tgccgtcggc tcctcggaca tccccgaaga ggggctcgag gcgctctgcg cggcaggcgg
     3901 caggaccgcg gccttcctcc cgctcaaccc gcttcggagg cgctggtcgg ctcacctgcg
     3961 caaccaccgc aagctcctcg tcgtcgacga ggagatcgcg ttcaccggcg ggatgaacgt
     4021 cggcgacgag tacgcccgcg gcagccggct cgccgggggc tcgggcgggc cgctccgcaa
     4081 ccgcccgtgg cgggacgccc acctgcgggt cgaagggccg gccgtcttcg acctcgcgac
     4141 cgtcttcgcc gaggactgga gcttcgccac gggggagcgc ctcgtccccg ggaggatccc
     4201 gatgccgacc gcggacggct ccgtcgtggc cgtcctcccg agcgggccgg accagacggc
     4261 gaacgcgaac gcccacgtct acttctcggc cctttccggc gccctcgagc gctgctggct
     4321 gacgaccccc tacttcgtcc ccgacgagcc ggcggcccgc gccctcgcga acgcggcgct
     4381 ccggggcatc gacgtccgcg tcctcgtccc ggccgagagc gacgtccctc tcgcccgcgc
     4441 ggcgggccgc ttcttctacg gctcgctcct ccgcgccggg gtccgcatct tcgagtacgt
     4501 gcccgccgtc ctccacgcca agaccgtcgt cgtcgacggc tcgtgggcgc tcgtgggctc
     4561 ggcgaacttc gacttccgca gcttcgcgct caacttcgag ctcggcgccg tcgtctacga
     4621 ccgggccttc gccgcgctcc tcgaggagcg cttcatccgg gacctcgccg aatcccgcga
     4681 ggtgacgctc gccgccgtgg agcggcgcgg cttcgccgag cgggcccgac tgggcctcgc
     4741 gcgcctcgcc gcgccgctcc tctgaggacc tcccacgcat gcgactccgg tccgcgccgt
     4801 ccgccctcct cgccctcctc ctcgccgcga ctccgctcgc cgcggccgac ctcccgatcc
     4861 acgagatcca gggcgcgggg gcgtcgagcc cgcgcgtcgg gcaatccgtc acgacgaccg
     4921 gcgtcgtgac cgccctccgg acgaacggct tctatctcca ggcccccgac gccgaggccg
     4981 acgccgaccc ggcgacctcg gaaggcgtcc tcgtcttcac ctcctccgcc cctcccgccg
     5041 ccgcggccgt cggaaaccgc gtccgcgtga gcggcagcgt cgtcgagtac cgcccctcgg
     5101 ccgaccccgc cagcccgtct ctcacggagc tcggcgggag cgtctcggtc gccctcctct
     5161 ccaccgggaa cgcgcttccc gctccggtcc tcctcaccac cgcggacctc tcgcccgctg
     5221 gccccgccga ccgcctcgag cggtacgagg ggatgcgcgt ccgcgtcccg cagctcctcg
     5281 tcgtcgcccc gacgggcggc tccgtcaacg aggaagcggc gacgggacgc tcgaacggcg
     5341 tcctcgtcgg cgtcctgccg gggacacccc gtcccttccg cgaggccggt gccgacccgc
     5401 tgacgccgct ccccgccggg agcccctgct gcgtcccgcg cttcgacggg aaccccgagc
     5461 ggctccgggt cgacaccgac ggcctcgtcg gcgtcgcccc gctcgacgtc gcctccggga
     5521 cgacgctcca cgacgtcgtc ggaccgctcg actacgcgtt tcgcacctgg acgctcctcc
     5581 cggagagcgc ccccgtcgcc accggttctc ccgccgcgcg tcccgtcccc gtgccgtcgt
     5641 ccgacgagct gacggtcggc tccctgaacc tcgagcgctt cttcgacgcg gaggacgacg
     5701 gcgaggggcc gaccctcaac gcggccgcct tctcgatgcg cctcgcgaag gcctccctcg
     5761 cggtccgtgc cctcctgcgc acccccgacg tcctcgccgt cgtcgaggtc gaggacctct
     5821 cgacgctccg cgcgctcgcg gcgcgcctct cggccgacgc cctcgcggcg ggcgctcccg
     5881 acccggcgta ccaggcgttc ctcgagggag gcaacgaccc gggcggcatc gacgtcggct
     5941 tcctcgtcgc ctcgcgcgtg tcggtccttt ccgtcgtcca ggaggggaag gcggcgacct
     6001 acaccgaccc gacgaccggg aacgcggcca cgctgaacga ccggccccct ctcgtcctga
     6061 ccgcccggtg ggccgcgccg gacgggaccg acctcgtctt caccgtcgtc gcgaaccacc
     6121 tccgctccct gaacgactcc gagctcgact cgacgacggg ccggcgcgtc cgcgcgaagc
     6181 ggcaggcgca ggcggagtac ctcgccgcgc tcctcgcgcg cctccaggcg gagggcggcg
     6241 atgcgccgct cctcgccgtc ggcgacttca acgccttcga ggtcagcgac ggtctcgtcg
     6301 acgtgatcgg caccgtccgc ggcgccccgg caccggccag cgaggtcgtc gtcaaccgaa
     6361 gcgtcgacct cgtcgccccc gacctcgtcg acctctgctc cgtcgacacc ctcgttcccc
     6421 cctccgagcg ctactcctac gtcttcgacg gcaacgccca gctcctcgac cacgccctcg
     6481 cgtcgggggc gacgctcccg ctcgtccgcg gcgtcgcctt cgcgcgcctc ggcgccgact
     6541 tccccgagac gatgcggggc gacgcgtcgc gccccgagcg cctctccgac cacgacggcc
     6601 tcgtcgttcg gatcggcctc gccgccccga agggacgctc ccctctcgtc cccgtccggg
     6661 acggagagcc gccgtcaccg ttccggtcgc ggactcccga gacggcgccg tgagccggga
     6721 ccccctcgcc cgactcgagg aagcacgacg ctctcgcccc ggtgcgcgcg cggccgaggt
     6781 ggcgctcgag tcggctgccg tgttcgtccc gcgcgacgcg acggagctcg cccggctcca
     6841 cgacgccgcg ctcttcctcc gcgtgtaccc acaatcgccc cgcgtcctcg ccctcgccga
     6901 ggcgctcctc gcgcgcgttc ccgcacatgc cgcgcgcctc gccggcgaag gggccgacct
     6961 cgccccgctc gacgacccgt ccgtcgcggg catcgccggc gttccggtcg cgatgccgtt
     7021 ctcgcacgcc gccgcctcct ggctcgcgcg acgcgacccg tcggctctcc gcgtcgactg
     7081 ggacgccgag gacctccccg accgcctcgc ggcgttcctc ccgcgcttcg ttccgttcct
     7141 cgaggagcag gcgcgcgtcg acgcgaacgt cgccttccgc gactgggtcg acgccgcccg
     7201 cggcgcgcgg cccgacgccc cgtggctcct cgagaggctc gccgcgttcc ccctgcccga
     7261 ttccgtccgc gccgagctct gggaggcgct ctccctccag ctcgtctgga cgccgcgcgc
     7321 cgcggactcc cgcgggctcg cgcgcgtccc gtcgcccgcc cccttctgct ccgacacgcc
     7381 gctcctctcg cggcgcgacg tctccgtcgc caccgagctc gccggcccgc cgccgagact
     7441 cgtccgcctc tcccgccgcg agggcgcggc gctcctcgac gcggcgcgcg cggcgatggc
     7501 catccgttac cgcgagctcc cggccttctc cgccggcgat ccgtcggccg tcttccgctt
     7561 cgacgtcggc cgcggcgtct ccatcttcgt caccggcctc gcccgcgacg cccgcctccc
     7621 gctccgtgcc gcgttcggga cactcttcgc tcggaacggc gtcctcgtcg gctacggcga
     7681 cctccacgct tccctcggcc gcgccgacgt cagcttcaac gtcttctatg cgttccgcga
     7741 cggcgagtcg gcctggctct acgcccgcct cctcgcggtg gcccgcgccg tcacccacgc
     7801 cgcgcagctc tccgtcgacc cgtaccaggt cggtctcggc aacgaggagg cgatcgcctc
     7861 gggcgccttc tggttctacc ggaagctcgg attccgctcg gccgaccccg ccctcgagag
     7921 gctcgcccgc cgcgaggagg cgcgcctcgc cgccgctcct tcccgtcgca cgcccgcggc
     7981 gaccctccgc cgcctcgccg tcgccccgct cctcctcgac gtcccgggcg agaccccgct
     8041 cctgccggac tcgttccgcc tcgccgacct cggccgcgcg gccgcgcgcc gcttcgccgc
     8101 ctcgaagctc tctcccgaag ccttccgcgc ccgggccgcc ggccgcgccg cgcgcgccct
     8161 cggcctcgat cccgtcgccc ctgcatgccg cgcgtctctc gaggcccttg ccccggctct
     8221 cgccctcctc gacgacctcg cttcctggcc gctctccgac cgccgcgccc tcgtggccct
     8281 cctccgcgcg aaggccgccc ccgacgagct ccgctacgcg cgcctctcct ccgggcaccg
     8341 ccgcctcgcc gcgtcgatcg cgcgagcagt gtcgggctga gggcggctcc tctcggacgg
     8401 tcgggtccgc tccgagcacg acgccgcggg ttcgggaagt ccgatgtgct aggttcgcgc
     8461 cgggggagta gacacgcgac cggcccgggg agcgatgtca tgacgacgcg gcggctcatt
     8521 cctctcgtac tcctcgtcgc gttcgcggcg gcggccgggg acgcccgcgc tcaggaccgg
     8581 acggcggcgc agcgggaggt cgaccggatc ctcgccggca agggaaccgc tcgcggcgag
     8641 gccgcgtgcg acctcctccg ttccggggtc ctggccaacg cgttcggagc ggcggcggcc
     8701 tcggcgagct acaggccggg ctcccgctac gtcccgaacg tcctctgcac ggcgagctgg
     8761 gccggggcga agggcacggc cgccgggtct cctcccggcc acgaagtgag cctgacgatc
     8821 ctggaccggt cgttcccctc ccgggccgct gccgtcggga gcctcgagag ctcggtggcc
     8881 gagctctcga agggggtcac cgtcgaggtg aagggccgca agcacacgag ccgggtggag
     8941 ttcgaaggct ggctggaggg cgtgggggac aaggcggcct gggcgccgaa gccccgcgag
     9001 ctttccgtgg cgtacggggg cgtgcggttc gccgtcgccg tccgaggctt cggcgacgcc
     9061 gccgaggaga aggcccgcgc gatcgacctg gcccggcggg tcgcgacttc gctcggccga
     9121 tgatccccgc cgccgtctcc cgcccgggcc cggccggccg cgaagcggcc cggcgccgcg
     9181 gggccctcct tgccgcgctc gccgccctgc tcgcgcttcc ctcggcgcgg gacgcgcggg
     9241 cccaggcgag cgccgccccg gccgtcgaga tcgtcgggcc gggaaggctc tccgaggggc
     9301 gcctccacca gtggtccgtc ttcatgcccc ggcgccacct cagcgagggg ccggacggct
     9361 acacgccgaa cggcctcgac gcgcccgccg acggcgcccc ccttccgacc acgtgggaac
     9421 gacccgtcgc gttccactgg gacttcggcg acggcacgcg cgtcgtcacc ggcgagcggg
     9481 ccacggcggg ccaccgctac gaggacgacg gcgcgttcac gatcaccgtc cgcgccgtcg
     9541 acaccgcggg gcgggaggtc gtcgggacga agccggtcac cgtgacgaac cgtccgcccg
     9601 gccgggtcga gctcttcgcg gtcgaggtcg acccggccga gtccctcttc gagctctcgg
     9661 cggagacctc cgacgtcgcg ggagaccccc tgacgatcga gtgggacttc ggcgacgggg
     9721 aaaaggcgaa ggggccgctg ccggagcgct ggaaggtccg gcacgccttc cccctgccgg
     9781 gccgctatcc ggttaccgtg aaggtcgagg acggcgacgg ggcgacccgc gaagcgacca
     9841 cccacgtcgt ctcgcgaggc ggatccgcgc aggaggagac gagcgtcgac gcgctggagg
     9901 acggcgggtc gaccacccgg aacacggtca gccgcttcga cgcgacgatc tcggggagcc
     9961 tcgccgcgtc cctcaccggc gaggtccgct cgctcgccgg cctccacctc tctcccgtcg
    10021 acggaggacg gcggtgccgc ttcctcctga cggcctggga ccccacgcac ctcgcgtccg
    10081 tcgccgtcat cctggacttg ccgggcctgc ccgaggggaa gggggcccgc tacacctttc
    10141 ggccgggccg cgtcgacctg aacctcgatc ccaccgccga gcggtatgcc ttcacgcggc
    10201 gggggctcct ccaggggttc gcggattctc cgctcgccga gaggatcgtc cccggcgtgg
    10261 acgagaggct cggcctcgat ccggcgcctc ctgcggcggc gccgccagcg ggcgacccgc
    10321 gccccgcccc ggccgtctcc ccgttcggcc tcgaggacca cgaggggttc cgtgccagcg
    10381 gtgggacgct cgagctcacg ttcgtgccct acgatcgggc gacggcccgt ttcgatctca
    10441 ccctcacgaa cctcgaccgg aagagcccct acccgggcct ctcgttcaaa gggagcttcg
    10501 cgctcgacct cgccgcggcc cggcgggacg ggctgatgaa ctacgaccaa tgcgctcccg
    10561 cacgcctcga ggtcgaggag gttcggccgg agccgggcac ccggcactcc gacggagaaa
    10621 accagtgggt cacggcccgc ttctcggggg acatcgaccc ggcgaccctg aacgaggaga
    10681 cgttccagct cacctggccg gactcctccg gggcgctcgt ccccgtcgaa gcgcgggtcc
    10741 accgccgggg gtcacaggcg ctcctcgtcc cgacccgacc cctgcgcgcg ggcgtgcgcc
    10801 acaccgcgcg cgtgaagacg gggcccgcgg gcgtccgctc ccgcggcgga gccgcgctcg
    10861 aggacaccga ccgctccggc tggcacgcgt gggacttcac gacccgcgtc gacgtcgtcc
    10921 cgaaacccgg cgccggaggg aacctctcct gccacgtctt ccagaccgtc cgcgacgcgc
    10981 cgctcgtcgc cggcaagccg gccgtggcgc ggatctacgc cgactggacg gagcaccccg
    11041 aggtcgatcc cggcgcgcag gtgcgcgact tcacggcgcg ggtcgtcctg cgcgaggtgg
    11101 tcggcacgac gagccgggag gtggcctcgg agctccaccg cttcgtcagg cccacgctcc
    11161 acgactcctt cggtgtctcc cgcaggaagg ccgagcacac cgcgaacgtc ttcttcactc
    11221 ccgagtcctc cacgccggcc tctctcgtcg tgggcctcga ggtgccacag gcgccctccg
    11281 agagccacgc cgaggcctac cagacccggt gcgccacgcc gatgtgggag cacgcgccga
    11341 cgctgaaggt ccgctactac ctcctcgccg tcaacgagtg gacgggcgag gagagccggg
    11401 cgcgtttcct ccccgtggcc acggacatcc tcgccgaagc cgagcggtgg gccgagcagc
    11461 tctttccctt ccgaagcgtc cagttcgagt acgggggcgc gctctctatt ccgcccagag
    11521 tgaagacgat tcccgtctac catgagccgg gcgacccgcc gccccctccg atctgcgacg
    11581 tggcctgcgc cacggaggtc ctccggctcg agaccgcgcg agcgcccgcc gacgtcgtca
    11641 tcggcctcat gccggtccag ggcgcctaca agggcggcgg ggcgttgacg aaggtcggcg
    11701 ggcaggaggc gcccgcgctc atccggctca cgctcgacga cgtcccgaag caccgggacc
    11761 gttgggtctt cgcggtcgtc cacgagatcg gccacacgct cggcctcgag cacctcccct
    11821 atctgggcgg agcgcaggag gatgccagct acgagcgcga cgtcctcgag gcgctgcgcg
    11881 accactcctg ggcgaacgcc gggaagccga tccactggta cgaggggatc gaggggttcc
    11941 ggatcgagcg cgacggccgc cgcggctaca acaagtcctc ggtcgaaggg aacgccgaag
    12001 gctcctggct cgtccccctg atgttcccca cgtcgatgct ctacagggac gccttcatcg
    12061 tgcgccacca gtacttcgac ctgctcgaac gaatcggggc gtggaagggg ccggcgagcg
    12121 ccgccgaagc tccctgacgc gctcaccgcg gccgtcgcgc gggccgcttc ggcgacccgc
    12181 cggggcggca cgctcagccc gcgagcggct tgccgtagat ccggtaccgc ttgcagacgg
    12241 tcgcgccgag gcgctccatc tcggcccgca ccctgaggtt cgtctcgagc tccacgtggc
    12301 tgtcgaagct cgtgaagccg gcggcgatcg cggaccggat caccgcggcc agcccgagga
    12361 tgtcggcgcc cttgccgcgg tcctcggccc gtattcctcc gagaagaagg tccagctgcc
    12421 tcgaggcgcg ggccgcgcgc aggacgtgga cgaacccgat cgggaagagc cgccccttcg
    12481 ctttccggag gcccgggtcg aggttcggca tcgcgacgag gaagccgatc ggcgtctcgc
    12541 ccttcattcc gaccttcacg aagcgcgggt cgagcagcgg aagatatttc cgggcaaggg
    12601 cctcgatctc cgcctcgtcg agcgggacgt aaccgtagag ctccgcgtag gtctcgttca
    12661 tcagccggat gatcgtggcc gcccaggggg cgagctcctt ccggctccgg aactccagga
    12721 tcctgtactc cgagcgggcg ccgacgcggg ccgcgacctt ctcgtagaag ggggggagcg
    12781 tcgggccgat cgccgccttg tagacgacga agtcgatctc cttgccgaag ccgcacgcct
    12841 ccacgaggcg tccgagccac gggtggttcg cataggtcgc gatcgtcggc tcgtgctcga
    12901 acccctcgat ccagaggccc tcggggtcct ggtccgagaa gcccatcggc ccgacgagcc
    12961 gcaccattcc gagccccttc gcccaccgct ccacccactc gaggagagcc cgcgcgacct
    13021 cggggtcctc ggggcactcg agccagccga accgcgcgac ccgggtgcct cgcgccgcga
    13081 cctcccttcg gctcacgatt ccgacgattc gcccgcacgg cttctccccg cggaacgcga
    13141 gcgcgaacgt ggtgtcgcag gtcgcgagcg cggggttcct ccgcgggtcg agcatcgcct
    13201 tctcctcgga gatgagaggc ggaacccagg tcgcgtgccc ccggtgaagc tcgaacggga
    13261 gtcggacgaa cgcccgccgc tcccgcgccg tggcgacctc ccggatcgtc acggcgttcg
    13321 tcattccctc acctctcacg cgggcgccat cgcgggatca taggcgcgcg ccggccgtcc
    13381 ggggacggca cttccttccg cgaagaggct ccctaccggg agaagtcgcc ggccgcctcg
    13441 ggctggtaga ggatcgcctc gacccgcaga cggcgcgggc cgctcgggac gacccactcg
    13501 atctcgtcgc cgacgcggta gccgagcatg gccgcgccga tcggggccac gacggagatc
    13561 cggcccgccg cgaagtcggc gtcctccggg tagacgacgg tgagctcgag cgtcgtccgc
    13621 tggtcgaggt cgagcagccg ggcccggctg ttcatcgtga tcacgtccgg agggatctcc
    13681 gaagccggca ccacgaccgc tcgctggacc tcggcgacga ggtcggccac gtcgtcccgg
    13741 tccatcgcgt cggtcggccg cgacaggagg gcctgcaggc gcttgcggtc gtcggacgtc
    13801 acgtagatgt ttcgatcgtt catgtcggtc tcctcctcgt cgggcgttcc cgggacaggc
    13861 caatggggga gcggggggga cgccccccgc gagcgaagcg aggtcggtgc tcttcgcagg
    13921 gagcaggcgg atcgcagagg cgcatacggg attcgaaccc gtgctaccgc cgtgcggggg
    13981 gagcgggggg acaccccccg cgagcgaagc gaggtcgtgc ccggtcggtg ctcttcgcag
    14041 ggagcgggcg gatcgcaaag gcgcatacgg gattcgaacc cgtgctaccg ccgtgcgggg
    14101 ggagcggggg gacacccccc gcgagcgaag cgaggtcgtg cccggtcggt gctcttcgca
    14161 gggagcgggc ggatcgcaaa ggcgcatacg ggattcgaac ccgtgttacc gccgtgcggg
    14221 ggggtgcggg gggtctcccc ccgccatcaa ggacgcccgg ccgcgccgcc ctctcacggc
    14281 gggaactgcc actgtcgaaa ctcgaggggt agggccagag ttggtagcgc atacgggatt
    14341 cgaacccgtg ttaccgccgt gagagggcgg cgtcctggac ccctagacga atgcgccgtt
    14401 ccggtcccgc tcgcgggggc tccccgcgcg aggacgggct ttttaccatg cctcccgtcc
    14461 ggcggcaagg ccggccccgg cgagccccgg ggccggcggc gtccggctca gtcggccgcc
    14521 cgcacccagc ggatgatctc ggggaaggtc ggcttcttgc cgtacatcag gatcccgacc
    14581 cggtagaccc gccccgcgaa ccaggagacg ccccagatcg tgaggaggag gagcccgatc
    14641 gagagggcga tctcccaggc cggcggcggc tgcaccgaga tccgcatgaa catcaggagc
    14701 ggcgaggtga aggggaagag cgagaggacc ctcgagaacg tcccgtccgg cgtgttgtac
    14761 gcgaagatcc aggtcatcgc cgacaggatg agcatcatcc cggggatcat gacgagctgc
    14821 tgcgcctcct gctcggagtt gaacggggcg gcgagggcgg cgtagagccc ggcgtagagg
    14881 aagtagccga ggaggaagaa gacgatgaac gcgacgagcg tcccgacggg gacgctcggg
    14941 agcgaaccgc tcgccagccc cacgaagccg acgattccgg ggagcgtcag ggtgaacgcg
    15001 aacagggccc agacggcgta ctgcgtcagc cccacgaggc cgattccgac gaccttcccg
    15061 agcatcagct ccgtcggctt caccgacgag atgatgacct ccacgatccg gttgctcttc
    15121 tcctcgagga cgcccctcat gacctgggcg ccgtagacga agatcgacat gtagatcagg
    15181 aagaagagga tcccggtg
//
LOCUS       DAOONL010000081        15092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4204_length_15092_cov_15.353661, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000081 DAOONL010000000
VERSION     DAOONL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15092
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_15092_cov_15.353661"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1416)
                     /locus_tag="PLP50_11895"
     CDS             complement(250..1416)
                     /locus_tag="PLP50_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52292.1"
                     /translation="MNYVPRRIEPVLRRAARDFPAVILTGPRRSGKTTVLKRVFPRAS
                     YVLLEDPDVLSRVKADPRGFLEGLKLPVVLDEIQNAPDLLGYIRTLVDASPRGMGKWL
                     LTGSQESPLIRGVSESLAGRAAVLQLWPLSVEESPKVAPLRGGFPEVLARPRSARTWF
                     SSYIQTFLERDVRSVAAIRDLGAFRRFLGLVATRHGQIVNRTDLAAPLGVSVPTITSW
                     LDILELTGQVLLLPPYFENFGKRLVKSPKLYLADPGLACFLLGIESERQLDASPFLGP
                     IFEGHVASEIVKSQLNQGKRRELYHFRDQKGLEVDFVVPGGEGRITLVEAKGTRTVSP
                     DLARSLVALGAAAGGTLPPAAFVVHRGAAAGVPSAALRPGVRSVPLPGLLAALR"
     gene            complement(1514..2797)
                     /locus_tag="PLP50_11900"
     CDS             complement(1514..2797)
                     /locus_tag="PLP50_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA52293.1"
                     /translation="MSETLSGRLLVLLSDGPARASVAALRRAAGRQVATTLDVRSGAL
                     PPEQIAGADAVLFQRLGVALVNVDPRDTERAARIRAAAEENAARILAVEPERRVAAYA
                     GAPRRLASGPAGSRSGARRSAIATRSPARRGPPGALPPAGSETTATWGLAATLVPGSK
                     YSGKKVKVAVLDTGFDLKHPDFPSRSVTAVSFVPGAKPQDGHGHGTHCIGTACGPKEP
                     FHPPRYGIAWGASILAGKVLDDNGGGADGGILAGIEWALAQGARVISMSLGAEITVDT
                     PFSEVFEAVGQRALAGGALIVAAAGNESDRRQGWYAPVGHPANCPSILAVGAVDVKTQ
                     PANFSNRGLVPAGGQVDLAGPGVDVLSAFPMPLRSRRLSGTSMATPHVAGLAALWAEA
                     KPKATATELWTLLTQHARRLPWLSTDVGSGLVQAP"
     gene            complement(3050..4513)
                     /locus_tag="PLP50_11905"
     CDS             complement(3050..4513)
                     /locus_tag="PLP50_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52294.1"
                     /translation="MRGDPFGSLAPLLTAVAAAPLPPVLVVLGDDEWFVGEAARRLAK
                     AFQAAFPDGEVSEYDGTGNGVREAVADAATIALFATNRLVSLDVTDLLRTKKLSADEV
                     DTLLDDAAEAGLGAPPGAPSEPRVLERLARKARALARETGAWVEGEAAESARKLTGRV
                     KRSDRAPELARLLDWPVDDGEAAETALGRLLEYVTRAGAGDNVLLLTAVSPDPEHAVL
                     SELARAPRAVLLARDDAARRERLHALGLERVLERGKTAEPDVFETLTERGRLSARAFL
                     SELDRLVDTAVGNRVTSEAAARLVVDERKEYGSDFVEAVARRRFLEALRIAERLLAAD
                     DFTAFRPFGKDGPAAPAKKGPKGEAAFFPLLGLLGAELRRMLALKAALAEPGADPGPR
                     RADYRAFVDRLLPRLKSSRPGAAPIPLDGHPFVLHKAYLASFDWPLDELADALAGLAD
                     LDRGVKTGLGSGPELLQTWLLLREKGSGLPSTLYGKP"
     gene            complement(4531..5025)
                     /locus_tag="PLP50_11910"
     CDS             complement(4531..5025)
                     /locus_tag="PLP50_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPA52295.1"
                     /translation="MATKSASAAKKAPAKSAAKTPVKAPAKVPARPPAKAAPKAAEKA
                     AEKAAKDPLEKHRKRLLAKQEELLHDLSRNRQVTDESVDEQAQDMVDRATSAYTREFA
                     FSLSESDRRTLLLIDQALLRLDQGTYGTCVHCQGHVQEKRLEAVPWARHCLECQELQD
                     KGLL"
     gene            complement(5156..6109)
                     /locus_tag="PLP50_11915"
     CDS             complement(5156..6109)
                     /locus_tag="PLP50_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52296.1"
                     /translation="MTLFATRHRGLAADLERLERGLGSDATILLLGGPGTGKDRLARL
                     LHDRSPRAAEPFVRIDLASLADDLLESELFGHERGAFTGAVAGKAGLLEGAGRGTLYL
                     DRIDTLSLRAQGKLLRLLEERLLRRVGGTRETLLEARFVASAAPDLPRRVRAGEFRED
                     LWYRLAVLTVRLPPLAERRADLLPAARALLRATDAPKAFTPEAVAALRAHPFRGGFRE
                     LENVVRRAALEASAAGAPAVDEPHLGLVSAAEPEALLESAASRGWPLRRLTDAYVRLV
                     LDEAGGNVAEAARRLGIARKTLYEHLGSGLPSTLYGPGRRV"
     gene            complement(6129..6761)
                     /gene="rsfS"
                     /locus_tag="PLP50_11920"
     CDS             complement(6129..6761)
                     /gene="rsfS"
                     /locus_tag="PLP50_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPA52297.1"
                     /translation="MKGAKPAAKPAGKPAAKRPAKPAVKARGPVEAKAAGARPAAPRR
                     RPALPPPPPPAAAVGEILSEAMAKGGHAKRVPAATAAAEKARLERNREADARLQMQIR
                     RAVQALLDKKAEKLVVLNLQELGAFAEYFVLASATNERQAQAMSDAVEMAMKAEGRRP
                     FSHEGYNKGAWILLDYGEVVFHLFHEEARRFYGLERLWGDAPEETQAFTS"
     gene            complement(6758..7420)
                     /gene="nadD"
                     /locus_tag="PLP50_11925"
     CDS             complement(6758..7420)
                     /gene="nadD"
                     /locus_tag="PLP50_11925"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPA52298.1"
                     /translation="MLNRAHLLGVYGGSFDPIHLGHLLPVEEARAATGLDEVLFVPAF
                     APPHKPQGPSASAFHRFGMCAAALEAYPHFLLSDFEVSRGGTTYTIETLRHVRANHPD
                     SEVFLVLGSDSMAQLHTWREWKEIVASHRIVVVYREGVDYPDLREGLPPELSARLAPL
                     GARPNDELADNQTVFWAGNAPVTISSTWIRSALPSVQSLSESLPPAVERYARRHRLYV
                     AP"
     gene            complement(7413..8474)
                     /gene="obgE"
                     /locus_tag="PLP50_11930"
     CDS             complement(7413..8474)
                     /gene="obgE"
                     /locus_tag="PLP50_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018649273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPA52299.1"
                     /translation="MFLDEASIQVSAGDGGNGCVAFRRAKGEPHGGPNGGDGGDGGSV
                     RIVADEGLNTLYPFRFKRRFAAGRGQHGMGSNCTGKKGGDIQITVPVGTIVKDADSGE
                     VLGDLAENGQVLLVAKGGRGGRGNQHFATPTHRTPRRAEPGTEGERRSLALELKLLAD
                     VALVGFPNAGKSTLIAAISAARPKIADYPFTTLVPNLGVVSWERYKTFVAADVPGLIE
                     GASEGHGLGIRFLRHVERSRLICHLVDAAALDPDDPDGAAEKAVAVIEGELEAFAPEL
                     AARERVLCANKMEIASEEQVAAVRAAASRRGLRFFAISGAAHKGLDPLVSFLGGRLEA
                     MSAKPAPVLPIEAAEAPDA"
     gene            complement(8533..8820)
                     /gene="rpmA"
                     /locus_tag="PLP50_11935"
     CDS             complement(8533..8820)
                     /gene="rpmA"
                     /locus_tag="PLP50_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPA52300.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFAGQWVTGGSILVRQRGTP
                     LKPGENVGRAKDDSLFAMVDGIVDFKDKGRLGRYVSVTPGPCPGSEVPAAS"
     gene            complement(8842..9162)
                     /gene="rplU"
                     /locus_tag="PLP50_11940"
     CDS             complement(8842..9162)
                     /gene="rplU"
                     /locus_tag="PLP50_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPA52301.1"
                     /translation="MYAVIRTGGKQVRVAKGDVIQIERFSDKTFTKGDALELPEVLAV
                     GEGETLRLGTPLVAGATVHGVVVREMRGQKLIVYKKKKRKGYQRSHGHRQNLVEVRID
                     SISV"
     gene            9337..9957
                     /locus_tag="PLP50_11945"
     CDS             9337..9957
                     /locus_tag="PLP50_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA52302.1"
                     /translation="MTRPGVSGVFSTMIEVVGEIEAVPAVPEEASWAEALRSPFAALA
                     GGDAQALEAVWEVAGRRLYALALWRTGRPEDAADVVQEVFFRLASRREELARVATPHT
                     WLLAVTHHAAVDLLRRRGRQRSEPLECAAAVEAPAHDPGRRVEAERVSQHLSRLPAPQ
                     REAVALRHVEGYSYREIGRITGVPTFTAASRCRLGLSRLRRLMEEP"
     gene            9957..10292
                     /locus_tag="PLP50_11950"
     CDS             9957..10292
                     /locus_tag="PLP50_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52303.1"
                     /translation="MSERPLLSGFEPPHAPPGLREAALRAGRRGMLSPTSPDVWNRLW
                     RSPAARLAWSAAVLLLAFAHARIPRENRIDVTTAGLEPELSEVAQLSRIAENSPRKDA
                     GFTLEGGPL"
     gene            10289..11383
                     /locus_tag="PLP50_11955"
     CDS             10289..11383
                     /locus_tag="PLP50_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52304.1"
                     /translation="MNRSRTYVLGALALVIALGAALGVRLLRAEDHDTPQHPALNLVR
                     HTEAAPAARVPAPAALPAGPLEVPYWGSPAAMAEWPVAFRTNLDLLAPLGDGPANAAL
                     WLKDFMKAGGPRAAEAEAAMKRRVDGASDLGKVLPPDDPFLREAEPWADQATMRFYPD
                     ILPIRGFETPIPNLLFSLNVAKSWVSRALADPDAPGALDDCRRAIRWGRLLRQEGVTV
                     IQDLIGLACIRAGAQGIYDLASRRGDSQTALVAAIVLGEHAPQRLRTQQLVSKLSVHD
                     ESGFRITDRKVEELSEAIRALPDLRFRGEAIIQMAVVRSVGTRHQRELADEALRLGAA
                     TKDPVQAALVRWAQESTFSKSDLAKMAGAE"
     gene            complement(11468..12406)
                     /gene="trxB"
                     /locus_tag="PLP50_11960"
     CDS             complement(11468..12406)
                     /gene="trxB"
                     /locus_tag="PLP50_11960"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPA52305.1"
                     /translation="MPNKSHYTVAIIGSGPAGLTAAIYASRANLEPVVFEGVQPGGQL
                     TTTTEVENYPGFEHGIQGPELMDVTRKQAMRFGTEIANDTVVDADLSARPFTLALESG
                     AKVTCDALIVASGASAKYLGLPSEKKLMGHGVSACATCDGFFFRGREVAIVGGGDTAM
                     EEANFLTKFATKVWLVHRRNEFRASKIMAQRTLDNPKIEVVWDSTITEILGEPATGVS
                     GIRVKNVKTGDERVIPVQGYFSAIGHEPNTKLFRGKLDMDDVGYLKIQHPTTYTSVEG
                     VFAAGDVADHIYRQAISAAGEGCKAAIDAERWLAGK"
     gene            12551..13039
                     /gene="sixA"
                     /locus_tag="PLP50_11965"
     CDS             12551..13039
                     /gene="sixA"
                     /locus_tag="PLP50_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HPA52306.1"
                     /translation="MLLYLLRHAEAEALSPSGLDADRALTDAGQKRMKAVGRAIARME
                     PGFDAVLVSPLLRARQTAEAVTSACRFKDEAVVTESLLPGSDPELLLEELAASEWRTV
                     LVVGHEPHLGRLFGRLVSGRKETEVPMKKASLAVFEIHGHLGPDRADLRSYLPARLLE
                     RL"
     gene            complement(13205..14167)
                     /locus_tag="PLP50_11970"
     CDS             complement(13205..14167)
                     /locus_tag="PLP50_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA52307.1"
                     /translation="MSRPLRLDHAGALWHVTSRGNERREVFRDDADRREMLHVLGRVV
                     DLFGWQLHAWVLMGNHYHLLLGTPEATLSRGMRQLNGDYAGHFNRRHGRDGHVFQGRF
                     KAILVQREAHLLEVARYIVLNPVRAGIVASAAEWPWSSFHATAGLEPAPEWLDTSFLL
                     DYFGSRRGPAGAKYREFVEAGTGGSYDPWSQVVGQIYLGSASWAESARAGQAVSGKGR
                     EVPRRQREPVTPKPEEVLARFEEAFGVTLDELRQRPRLLSRERALLAAALRRHARATL
                     PVIAETFGVELSRASMLARRDGLEGASRDVRRKAAVFEKLLAER"
     gene            complement(14206..14793)
                     /locus_tag="PLP50_11975"
     CDS             complement(14206..14793)
                     /locus_tag="PLP50_11975"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPA52308.1"
                     /translation="MPIRINRVYTRGGDKGETSLVGGQRIAKDSIRIESYGTVDELNA
                     VLGLARVANRDLPGASDADRARLDALLFRLQNELFNLGSDLATLPADRHPKQPVVEPR
                     HVAALEADIDALNVGLPELTSFVLPGGGPVGAHLHQARTVCRRAERLVTTLARQEAIG
                     TLGLVYLNRLSDLLFVLSRWAARARSEPETLWRPE"
     gene            14847..>15092
                     /locus_tag="PLP50_11980"
     CDS             14847..>15092
                     /locus_tag="PLP50_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPA52309.1"
                     /translation="MRATDYHVHTARCGHAGGATRDYVLQAISRGLDEIAFTDHVPLW
                     FLPGDDPEPGLAMTRAELPEYVEEVLALREEFRGRIDV"
BASE COUNT         2097 a         4962 c         5875 g         2158 t
ORIGIN      
        1 ccaggatctc cagggtctcg aggagcccct cgtactcgtc gacggacatg aggactccgg
       61 cgggccggcc ggaccgcgtg atgacgaact gctcacccac ttcctcgacg cgggcgagga
      121 ggcgtgcaag gtgtgtcttc gcttcggaga gagggagcgt cgtcatgacc ttattttaga
      181 ccagaattct ggtccgagcg cggtctcccc ggttggggtc tggtctacat tctacagtct
      241 gcgagtcgtt cagcggaggg cggcgaggag cccgggaagc gggaccgacc tgactccggg
      301 ccgcagagcg gccgacggaa cacccgcggc cgcgccacgg tgaacgacga aggcggcagg
      361 cggaagcgtt cccccggcgg ccgctccgag ggcgacgagg ctgcgggcga gatccggact
      421 gacggtgcgc gtcccctttg cttcgacgag agtgatgcgc ccttccccgc ccggcacgac
      481 gaaatcgacc tcgagcccct tctggtcgcg gaagtggtag agctcccgtc gcttcccctg
      541 gttgagctgg ctcttgacga tctcggaggc cacgtgcccc tcgaagatcg gccccaggaa
      601 gggtgaggcg tcgagctgcc tctcgctctc gatgccgagg aggaagcagg ccagacccgg
      661 gtccgcaagg tagagcttcg ggctcttgac gaggcgcttt ccgaagttct cgaagtaggg
      721 cgggaggagg aggacctggc cagtgagctc gaggatgtcg agccaggagg tgatcgtcgg
      781 gaccgagacg ccgagcgggg ccgcgaggtc ggtgcggttg acgatctgac cgtgccgcgt
      841 ggcgacgagg ccgaggaacc tgcggaaggc gccgaggtcg cggatggccg cgacggaccg
      901 gacgtcgcgc tcgaggaatg tctggatgta ggagctgaac caggtgcgcg ccgagcgggg
      961 ccgggcgagg acttccggga agcctccgcg aaggggagcg accttcggtg actcctcgac
     1021 ggagaggggc cagagctgca ggacggcggc acgtcccgcg agggactcgc tgactccgcg
     1081 tatcagcggc gactcctggg agcccgtgag gagccacttc cccatgccgc ggggggaggc
     1141 gtcgacgagg gtgcggatgt acccgagcag atccggggcg ttctggatct cgtcgaggac
     1201 gaccgggagc ttcaggccct cgaggaagcc gcgcggatcg gccttgaccc gcgaaaggac
     1261 atcggggtcc tcgagaagga cgtagctcgc ccgcgggaag accctcttga gcacggtcgt
     1321 ctttcccgag cgcctcggcc cggtgaggat gaccgcgggg aagtcgcgcg cggcgcgacg
     1381 aaggacgggc tcgatacggc gcgggacgta gttcactttg ggaattctaa agccgcactt
     1441 taggaattac ccacgccgga atggggtctg gtctacactc cgccggaatg gggtctggtc
     1501 tacactctac agtctacggc gcctggacga ggccggagcc gacgtcggtg gagagccagg
     1561 ggaggcgccg ggcgtgctgg gtgaggaggg tccagagctc ggtggccgtg gccttcggct
     1621 tcgcctcggc ccagagggcg gcgaggcccg cgacgtgcgg ggtcgccatg ctggtgccgg
     1681 agaggcggcg gctgcggagc ggcatgggga aggccgagag gacgtcgacg ccggggccgg
     1741 cgaggtcgac ctggccgccg gccgggacga ggccgcggtt ggagaagttg gcgggctggg
     1801 tcttcacgtc gacggcgccg acggcgagga tcgacgggca gttggcgggg tggccgacgg
     1861 gcgcgtacca gccctggcgg cggtcgctct cgttgccggc cgcggcgacg atgagggcgc
     1921 cgccggcgag cgcgcgctgc cccactgcct cgaagacctc gctgaaggga gtgtcgacgg
     1981 tgatctcggc gccgagggac atggagatga cgcgcgcgcc ctgcgcgagg gcccactcga
     2041 tgccggcgag gatgccgccg tcggcgccgc cgccgttgtc gtcgaggacc ttgcccgcga
     2101 ggatggaggc gccccaggcg atgccgtagc gcggcgggtg gaagggctcc ttcggcccgc
     2161 aggcggtgcc gatgcagtgg gtgccatggc cgtggccgtc ctgcggcttc gcgcccggca
     2221 cgaagctgac ggcagtgacg gagcgcgagg ggaagtcggg gtgcttgagg tcgaagccgg
     2281 tgtcgaggac ggcgaccttg accttcttgc ccgagtactt cgagccgggg acgagggtgg
     2341 ccgcgaggcc ccaggtggcg gtggtctcgg agcctgcggg agggagggcg ccgggcgggc
     2401 cgcggcgggc cggggagcgg gtggcgattg cgctgcggcg ggcgccggag cggctgccgg
     2461 ccgggccgga ggccaggcgg cgcggggcgc cggcgtaggc cgcgacgcgc cgctcgggct
     2521 cgacagcgag gatgcgcgcg gcgttctcct cggcggcggc tcggatgcgg gcggcgcgct
     2581 cggtatcgcg ggggtcgacg ttgacgaggg cgacgccgag gcgctgaaag aggacggcgt
     2641 cggcgcccgc gatctgctcg ggaggcaggg cgccggagcg gacgtcgagg gtggtcgcga
     2701 cctggcgccc ggcagccctt cggagggcgg cgacggaggc gcgggccggg ccgtcggaga
     2761 ggaggacgag gaggcggccg ctgagagttt cgctcatggt aagctattca caggaaactg
     2821 gttacgcgag cggtgcccgg aatccggata gcctccggcc ttctgggtgg cagcagattt
     2881 cgctgcgccg ccccaaccgc cggtcgggcg tcaactcggt tcctggacgg gcacgggatt
     2941 cggtccaagg atagctcacg cccgactgcc gaaaatctcg gggaggatag gcgcctcgcg
     3001 gcacccgcgt caacggatga gggatggggt caggtctacc atctacactc tacggcttgc
     3061 cgtagagtgt agatggtaga cctgacccct tttcgcggag gaggagccag gtctggagga
     3121 gctcggggcc ggagccgagg ccggtcttga cgccgcggtc gagatcggcg aggccggcga
     3181 gggcgtcggc gagctcgtcg agcggccagt cgaaagaggc gagataggcc ttgtggagga
     3241 caaaggggtg gccgtcgagg gggatcggcg ccgcacccgg gcgggacgac ttgaggcgcg
     3301 ggaggagacg gtcgacgaag gcccggtagt cggcgcggcg cgggccgggg tcggcgcccg
     3361 gctcggcgag cgcggccttc agcgcgagca tgcgccgcag ctcggcgccg aggaggccga
     3421 ggagcgggaa gaaggcggcc tcgcccttcg gccccttctt cgcgggggcc gcgggaccgt
     3481 ccttgccgaa ggggcggaag gcggtgaagt cgtcggcggc caggaggcgc tcggcgatgc
     3541 ggagggcctc gaggaaacgg cgccgggcga cggcctccac gaagtcggag ccgtactcct
     3601 tccgctcgtc gacgacgagg cgcgcggcgg cctcggaggt gacgcggttg ccgacggcgg
     3661 tgtcgacgag gcggtcgagc tcggagagga aggcgcgggc cgagaggcgg ccccgctcgg
     3721 tgagggtctc gaagacgtcg ggctcggcgg tcttcccccg ctcgaggacg cgctcgaggc
     3781 cgagggcgtg gaggcgctcg cgccgcgcgg cgtcgtcgcg cgcgaggagg acggcgcgcg
     3841 gggcgcgggc gagctcggag aggacggcgt gctcggggtc gggcgagacg gcggtgagga
     3901 ggaggacgtt gtcgccggcg ccggcgcggg tgacgtactc gaggaggcgc ccgagggcgg
     3961 tctcggcggc ctcgccgtcg tcgacgggcc agtcgaggag acgggcgagc tcgggagcgc
     4021 ggtcggagcg cttgacgcgc cccgtgagct tgcgggcgct ctcggcggct tcgccttcga
     4081 cccaggctcc cgtctcgcgg gcgagggcgc gggccttgcg tgcgaggcgt tcaaggacgc
     4141 gcggctcgga gggggctccg ggcggcgcgc cgaggccggc ctcggcggcg tcgtcgagga
     4201 gggtgtcgac ctcgtcggcg gagagcttct tcgtccggag gaggtcggtg acgtcgaggg
     4261 agacgaggcg gttcgtggcg aagagggcga tggtggccgc gtcggcgaca gcttcccgca
     4321 cgccgttgcc ggtgccgtcg tactccgaga cctcgccgtc ggggaacgcg gcctggaagg
     4381 cctttgcgag gcggcgcgcg gcctcgccga cgaaccactc gtcgtcgccg aggacgacga
     4441 ggaccggagg gagcggtgcg gcggcgaccg ccgtgaggag cggcgccagg gagccgaagg
     4501 ggtcgccgcg caaagggagc ctctccgggc tcagagcagg cccttgtcct gcagctcctg
     4561 gcactccagg cagtgccgcg cccacgggac cgcctcgagg cgcttctcct ggacgtggcc
     4621 ctggcagtgg acgcaggtgc cgtaggtgcc ctggtcgagg cggagaaggg cctggtcgat
     4681 gaggagaagg gtgcggcggt cggactcgga gagggagaac gcgaactcgc gcgtgtaggc
     4741 cgaggtggcc cggtcgacca tgtcctgcgc ctgctcgtcg accgactcat cggtgacctg
     4801 ccggttgcgg gagaggtcgt gaaggagctc ctcctgcttg gcgaggaggc gcttgcgatg
     4861 cttctcgagg gggtccttcg cggccttttc tgcggccttc tcggcggcct tcggcgccgc
     4921 cttcgcaggc ggccgggccg ggaccttcgc aggggccttc acgggggtct tcgccgcgct
     4981 cttcgcggga gctttcttcg ctgcggacgc gctcttcgtc gccatgggga cattccccct
     5041 tccgtcgttc ccggacgcct cgcggcgccc gtccggcgag ccgaacccgc cgtgccggcc
     5101 cctccggaat cgcgggactc tagcacaaga ccgggattgg ggtcaggtct accatctaca
     5161 ctctacggcc agggccgtag agtgtagatg gtagacctga ccccaggtgt tcgtagaggg
     5221 tcttgcgggc gatgccgagg cggcgggcgg cctcggcgac gttgccgccg gcctcgtcga
     5281 ggacgaggcg gacgtaggcg tcggtgaggc ggcggagcgg ccagccacgg gaggcggcgg
     5341 actcgaggag ggcttcgggc tcggcggcgg agacgaggcc gaggtggggc tcgtcgacgg
     5401 cgggggcgcc cgcggccgag gcctcgagcg cggcgcgccg gacgacgttc tcgagctcgc
     5461 ggaagccgcc gcggaagggg tgggcgcgaa gggccgcgac ggcctcgggt gtgaaggcct
     5521 tcggcgcatc ggtggcgcgg agcagggcgc gagccgccgg gaggaggtcg gcccggcgct
     5581 cggcgagcgg cgggaggcgg acggtgagga cggcgagccg gtaccagagg tcttcccgga
     5641 actcccccgc ccggacgcgg cgcgggaggt cgggtgcggc cgaggcgacg aaccgcgctt
     5701 cgaggagggt ctcgcgcgtg ccgccgacgc ggcggaggag ccgctcttcg aggaggcgga
     5761 ggagcttgcc ctgcgcgcgg agggagagcg tgtcgatgcg gtcgaggtag agggtgccgc
     5821 gcccggcgcc ttcgaggagg cccgccttgc cggcgacggc gccggtgaag gcgccacgct
     5881 cgtggccaaa gagctcgctc tcgaggaggt cgtcggcgag cgacgcgagg tcgattcgga
     5941 cgaacggctc cgcggcgcgc ggggagcggt cgtggagaag gcgcgcgagg cggtctttgc
     6001 cggtgcccgg gccgccaagg aggaggatgg tggcgtcgga accgaggccc cgctcgagcc
     6061 tctcgaggtc ggcggccaga ccgcgatggc gggtggcgaa gagcgtcacg ccgctcgccc
     6121 cgcgggacct agctcgtgaa ggcctgcgtc tcctcgggag cgtcgcccca gaggcgctcg
     6181 agcccgtaga agcgccgggc ctcctcgtgg aagaggtgga agacgacctc gccgtagtcg
     6241 aggaggatcc aggcgccctt gttgtagccc tcgtgggaga aggggcgccg cccctcggcc
     6301 ttcatcgcca tctcgacggc gtcggacatc gcctgcgcct gccgctcgtt cgtggcggag
     6361 gcgaggacga agtactcggc gaaggcgccg agctcctgga ggttgaggac gacgagcttc
     6421 tcggccttct tgtcgaggag ggcctggacg gcgcgccgga tctgcatctg gagacgcgcg
     6481 tcggcctcac ggttccgctc gaggcgggcc ttctcggcgg cggccgtagc cgcggggact
     6541 cgcttcgcgt ggccgccctt cgccatcgcc tcggagagga tctcgccgac ggcggccgcg
     6601 ggcggaggag gaggcggaag cgcgggcctg cgccgcgggg ctgcgggcct tgcgccggcg
     6661 gcctttgcct cgaccgggcc cctggccttg acggctggct tcgcggggcg cttggctgcg
     6721 ggcttgccgg cgggcttcgc ggcgggcttc gcgcccttca cggcgcgacg tagaggcggt
     6781 gccgcctcgc gtaccgctcc accgcggggg gaagactttc gctcaggctt tggacgctcg
     6841 ggagtgcgct tcttatccac gtggaggaga ttgtaaccgg cgcgttcccc gcccagaaga
     6901 cggtctggtt gtcggccagc tcgtcgttcg gccgcgcccc gagcggtgcg agacgggcgg
     6961 acagctcggg cggcagcccc tcgcgtaggt cggggtagtc gaccccttcg cggtagacga
     7021 cgacgatccg gtgggacgcg acgatctcct tccactcgcg ccaggtgtgg agctgggcca
     7081 tcgagtcgga gccgaggacg aggaagacct cgctgtcggg atggttcgcg cggacgtgac
     7141 ggagcgtctc gatggtgtag gtcgtcccgc cgcgggagac ttcgaagtcg gagaggagga
     7201 agtgcgggta cgcctcgagc gcggccgcgc acatcccgaa gcggtggaac gccgaggcgg
     7261 aagggccctg cggcttgtgc ggcggcgcga aggcggggac gaagaggact tcgtcgaggc
     7321 cggtcgccgc gcgcgcctcc tcgacgggaa ggaggtggcc gaggtggatc gggtcgaagg
     7381 agccgccgta gacgccgagg aggtgggcgc ggttaagcat cgggagcctc cgctgcctcg
     7441 atgggcagga cgggagccgg cttcgcgctc atcgcctcga gccggccgcc gaggaaggag
     7501 acgaggggat cgagcccctt gtgggccgcg cccgagatcg cgaagaagcg gaggccgcgg
     7561 cgggaggccg cggcgcgcac ggcggcgacc tgctcctcgg aggcgatctc catcttgttc
     7621 gcgcagagga cgcgctcgcg cgccgcgagc tccggcgcga acgcctcgag ctctccctcg
     7681 atgaccgcga cggccttctc ggcggccccg tcggggtcgt ccgggtcgag ggccgcggcg
     7741 tcgacgaggt ggcagatgag gcgcgaacgc tcgacgtggc ggagaaaccg gatgccgagc
     7801 ccgtgcccct cgctcgcccc ctcgatgagg ccggggacgt cggccgcgac gaacgtcttg
     7861 tagcgctccc aggagacgac gccgaggttc gggacgaggg tcgtgaaggg gtagtcggcg
     7921 atcttcgggc gcgccgcgga gatcgcggcg atgagcgtcg acttgccggc gttcgggaag
     7981 ccgacgaggg cgacgtcggc gaggagcttc agctcgagcg cgagggagcg ccgctcgccc
     8041 tcggtgccgg gctcggcgcg ccggggcgtg cggtgggtgg gcgtcgcgaa gtgctggttg
     8101 ccgcgaccgc cgcggccgcc cttggctacg aggaggacct ggccgttctc ggcgaggtcg
     8161 ccgaggacct cgccggagtc ggcgtccttg acgatcgtgc caacggggac ggtgatctgg
     8221 atgtccccgc ccttcttccc ggtgcagttc gagcccatgc cgtgctggcc gcggcccgcc
     8281 gcgaaccgcc tcttgaagcg gaacgggtag agggtattga ggccttcgtc cgcgacgatg
     8341 cggacgctgc cgccgtcacc gccgtcgccg ccgttgggcc cgccgtgggg ctcgcccttc
     8401 gcgcgccgga acgcgacgca gccattgccg ccgtcgccgg ccgagacctg tatcgacgcc
     8461 tcgtcgagga acattcgatc gtccgcgccg gagaatcggg ctcggggcgc cgcgagggcg
     8521 ccccgcccgg aatcagctgg cggccgggac ctcggagcca gggcacgggc cgggcgtcac
     8581 gctgacgtag cgccccagcc gacccttgtc cttgaagtcg acgatgccgt cgaccatcgc
     8641 gaagagcgag tcgtccttgg cgcggccgac gttctcaccc ggcttgagcg gggtgccccg
     8701 ctgccggacg aggatcgagc cgccggtgac ccactggccc gcgaagcgct tgacgccgag
     8761 gcgctgggcg ttcgagtcgc gaccgttgcg ggaagaaccc tgaccttttt tgtgcgccat
     8821 agcagcttcc tcggaaggct ctcagaccga gatcgagtcg atccggactt cgacgaggtt
     8881 ctggcggtgc ccgtggctcc gctggtaccc cttgcgcttc ttcttcttgt agacgatgag
     8941 cttctggccc ctcatctcgc gaacgacgac accatggacg gtggcgccgg ccacgagcgg
     9001 agtgccgagg cggagggttt ccccttcgcc gacggccagg acctcgggca gctcgagcgc
     9061 atcgcccttg gtgaacgtct tgtcggagaa gcgttcgatc tggatcacgt cgcccttggc
     9121 gactcggacc tgcttccccc cggtgcgaat gacggcgtac atcgattcct cccccgacct
     9181 cgggtcaaaa gggtcggcaa acataccctc ggccgatcgg aatgtcaaaa cctcgggaag
     9241 agcgggggta gaggaggggg tctggtctac actctacact ctgctctcga gcagagtgta
     9301 gagtgtagac cagaccccgg ggtacgcgcg cggcgcgtga caagaccggg agtctccggc
     9361 gtcttctcta cgatgatcga agtcgtgggg gagatcgagg cggtccccgc ggtgcccgaa
     9421 gaggcatcct gggccgaggc gctccgctcg ccgttcgccg ccctcgccgg cggcgacgcg
     9481 caggcgctcg aggccgtgtg ggaggtcgcg gggcggcggc tctacgcgct cgcgctctgg
     9541 cggaccgggc gccccgagga cgccgccgac gtcgtgcagg aggtcttctt ccgccttgcc
     9601 tcgcggcggg aggagctggc gagggtggcg acgccgcaca cctggctcct ggccgtgacg
     9661 caccacgccg cggtggacct cctgcggcgg cgcgggcgcc agcggagcga gcccctggag
     9721 tgcgccgcgg ccgtggaggc cccggcgcac gacccggggc ggagggtcga ggccgagagg
     9781 gtctcgcagc acctctcgag gctccccgcg ccccagcggg aagcggtcgc cctccggcac
     9841 gtcgaggggt actcgtaccg ggagatcggc cggattacag gcgtgccgac gttcaccgcc
     9901 gcgagccgtt gccggctcgg gctctcgcgc ctgcggcgcc tgatggagga accgtgatga
     9961 gcgaacgtcc gcttctcagc ggcttcgagc caccacatgc gccccctggt ctgcgcgagg
    10021 ccgcgctccg ggcgggacgc cgcggaatgc tctcccccac ctcccccgac gtctggaacc
    10081 gcctctggcg tagccccgcg gcacgtctcg cctggagtgc cgccgtcctg ctcctggcct
    10141 tcgcgcacgc tcgcatcccg cgcgagaacc gcatcgacgt caccacggcg ggcctggagc
    10201 ccgagctgag cgaggtcgcg cagctctccc gcatcgccga gaactccccg cgcaaagacg
    10261 cgggtttcac cctcgaagga ggccctctgt gaaccgttcc cgaacctacg tcctcggcgc
    10321 cctcgcgctc gtgatcgccc tcggagccgc gctcggcgtg cggctcctgc gcgccgagga
    10381 ccacgacacg ccgcagcatc cggcgctgaa cctcgtgcgg cacacggagg ccgcccccgc
    10441 tgcccgcgtt cccgccccgg ccgcactccc cgcggggccg ctcgaggtcc cctactgggg
    10501 ctccccagcc gccatggccg aatggcccgt cgcgttccgg acgaacctcg acctcctcgc
    10561 gccgctcggc gacgggcccg caaacgccgc tctctggctg aaggacttca tgaaggcggg
    10621 agggccgcgt gccgcggagg cggaggccgc gatgaagcga cgggtagacg gcgcgtcgga
    10681 cctcgggaag gtcctcccgc cggacgaccc cttcctcaga gaggccgagc cctgggccga
    10741 ccaggcgacg atgcgcttct accccgacat cctgccgatc cggggcttcg agacgccgat
    10801 cccgaacctc ctcttctcgc tgaacgtggc gaagtcgtgg gtctcgcgcg ctctcgcgga
    10861 ccctgacgcc cccggcgcgc tcgacgactg ccggcgcgcg atccgctggg gccggctgct
    10921 tcgccaggag ggagtgaccg tcatccagga tctcatcggc ctcgcctgca ttcgggccgg
    10981 cgctcagggc atctacgacc tcgcctcgcg ccgcggggac tcgcagaccg cgctcgtggc
    11041 cgcgatcgtc ctcggggagc atgccccgca gaggctgcgg acgcagcagc tcgtctcgaa
    11101 gctctcggtc cacgacgaga gcggcttccg gattaccgac cgcaaggtgg aggagctctc
    11161 cgaggcgatc cgcgcgctgc ccgacctgcg gttccggggc gaggcgatca tccagatggc
    11221 cgtcgtccgg agcgtcggga cgaggcacca gcgcgagctc gccgacgagg cgctgcgcct
    11281 cggcgccgcg accaaggacc ccgtgcaggc cgccctcgtt cgctgggcgc aggaatcgac
    11341 cttctcgaag agcgacctcg cgaagatggc cggcgccgag tagaagctcg ttgggggggt
    11401 caggtctacc atctacactc tacgccgcgg cgtagagtgt agatggtaga cctgaccctt
    11461 atcgttctta cttcccggcc agccagcgtt cggcgtcgat ggcggccttg cagccctcgc
    11521 ccgccgcgct gatggcctgc cgatagatgt ggtcggcgac gtcgccggcg gcgaagaccc
    11581 cctcgaccga ggtgtaggtc gtcgggtgct ggatcttcag gtagccgacg tcgtccatgt
    11641 cgagcttgcc gcggaagagc ttcgtgttcg gctcgtggcc gatggccgag aagtagccct
    11701 ggaccgggat gacgcgctcg tcgcccgtct tgacgttctt gacgcggatg ccggagacgc
    11761 ccgtcgcggg ctcgccgagg atctcggtga tcgtcgagtc ccagacgacc tcgatcttcg
    11821 ggttgtcgag cgtgcgctgc gccatgatct tcgaggcgcg gaactcgttc cggcggtgga
    11881 cgagccagac cttcgtcgcg aacttcgtca ggaagttcgc ctcctccatc gcggtgtcgc
    11941 cgccgccgac gatggcgact tccctgccgc ggaagaagaa gccgtcgcac gtcgcgcagg
    12001 cggagacgcc gtgccccatc agcttcttct cactcgggag gccgaggtac ttggccgagg
    12061 cgccggacgc gacgatgagg gcgtcgcagg tgaccttcgc tccgctctcg agggcgagcg
    12121 tgaaggggcg ggcggagagg tcggcatcga cgacggtgtc gttcgcgatc tcagtgccga
    12181 agcgcatcgc ctgcttccgg gtgacgtcca tcagctcggg cccctggatg ccgtgctcga
    12241 agccggggta gttctccacc tcagtcgtcg tcgtgagctg gccgccgggc tgaacgcctt
    12301 cgaagacgac cggctcgagg ttcgcgcggg aggcgtagat ggcggcggtc aggcccgccg
    12361 ggcccgagcc gatgatggcg acggtgtagt ggctcttgtt cggcatctct tcctcggttc
    12421 ggtgcggcaa aggaacgggg tcgccgcaga gtgtagatgg tagacctgac cccaaggacc
    12481 gtcccggggg cgagcgattc tgcaccgaag gggggtcggt ggcgcgcggg gcgtgtgata
    12541 gcgtttttcc atgcttctct acctgctgcg tcacgccgag gcggaagccc tctctccgag
    12601 cgggctcgac gccgaccggg cgctcaccga cgcggggcag aagcgaatga aggcggtggg
    12661 ccgggcgatc gcgcggatgg agccgggctt cgacgcggtg ctcgtatcgc ccctgctgcg
    12721 ggcgaggcag acggccgagg cggtgacgtc cgcctgccgt ttcaaggacg aagccgtcgt
    12781 gaccgaatcg ctcctgccgg gctccgaccc ggagctcctc ctcgaggagc tcgcggcgtc
    12841 ggagtggcgg acggtcctcg tggtcgggca cgagccgcac ctcggacgtc tcttcggccg
    12901 cctcgtctcg ggccgaaaag agacggaggt gccgatgaag aaggcgtcgc tcgccgtctt
    12961 cgagatccac ggccacctcg gccccgaccg cgccgacctc cgctcttacc tccccgcgcg
    13021 cctcctcgaa cgcctctgac gcaaggggca cacccagggg ggctcaaccc ccggggcctg
    13081 gtctacactc tacagtctac gggccacggc gacccaaggg gtctgatcta cactctacag
    13141 tctacccgcc acgggcgacg ccccgcggaa gccgcacgcg ggcgggactg agcgtggctc
    13201 gccgtcaccg ctccgcgagg agcttctcga agaccgcggc cttgcggcgc acgtcgcgcg
    13261 aggccccttc gaggccgtcg cgccgcgcga gcatcgaggc gcgcgagagc tcgacgccga
    13321 aggtctcggc aatgacggga agcgtggccc gcgcgtggcg ccggagggcc gcggcgagca
    13381 gggcgcgctc gcgagagagg agacgcggcc gctgccggag ctcgtccagc gtgacgccga
    13441 aggcctcctc gaagcgggcg aggacctcct cgggcttcgg cgtcaccggc tcgcgctgcc
    13501 gccgcggcac ctcgcgcccc ttcccggaga cggcctgccc cgcgcgtgcc gactcggccc
    13561 acgaggcgct gccgaggtag atctgcccga cgacctggct ccaggggtcg tacgagccgc
    13621 ccgtccccgc ctcgacgaac tcgcggtact tcgcgccggc cgggccgcgc cgggagccga
    13681 agtagtcgag aaggaacgag gtatcgagcc attcgggtgc cggctcgagt cctgcggtcg
    13741 cgtggaagct gctccacggc cactcggccg ccgaggcgac gatgccggcg cggacgggat
    13801 tcaggacgat gtagcgcgcg acctcgagga ggtgcgcctc gcgctggacg agaatcgcct
    13861 tgaaccgccc ctggaagacg tggccgtcgc gcccgtggcg acggttgaag tggccggcgt
    13921 aatcgccgtt gagctggcgc atcccgcgcg agagcgtcgc ctccggcgtc ccgaggagga
    13981 ggtggtagtg gttccccatc aggacccacg cgtggagctg ccagccgaag aggtcgacga
    14041 cccggccgag gacgtgcagc atctcgcgcc ggtcggcgtc gtcgcggaag acctcgcggc
    14101 gttcgttgcc gcgggaggtg acgtgccaga gggcgccggc gtggtcgagg cgaaggggac
    14161 ggctcatgcc gccagtatag tgtagatggt agacctgacc cctctctatt ccgggcgcca
    14221 gagggtctcg ggctcggagc gggcgcgggc ggcccagcgg gagaggacga agagaaggtc
    14281 ggagagccgg ttcaggtaga cgaggccgag cgtgccgatc gcctcctgcc gggcgagcgt
    14341 cgtgacgagg cgctcggcac ggcggcagac ggtacgggcc tggtggaggt gggcgccgac
    14401 ggggcctccg cccgggagga cgaaggaggt gagctccggg agcccgacgt tcagcgcgtc
    14461 gatgtcggcc tcgagcgcgg cgacgtgccg gggctcgacg acgggctgct tcgggtggcg
    14521 gtcggccggg agcgtcgcga ggtcggagcc gaggttgaag agctcgttct gaaggcggaa
    14581 gaggagcgcg tcgagccgcg cgcggtcggc gtcggaggca cccgggaggt cgcggttcgc
    14641 gacgcgcgcc agcccgagga ccgcgttgag ctcgtcgacg gtgccgtagc tctcgatccg
    14701 gatcgagtcc ttcgcgattc gctgtccccc gacgaggctc gtctcgccct tgtcgccccc
    14761 ccgggtgtag acacggttga tccggatcgg cattccgttc tcctcccgcc gctccgtgcc
    14821 cgggcggcct gctaccctcc gccgcgatgc gcgcgacgga ttaccacgtc cacaccgccc
    14881 gatgcgggca cgcgggcggc gcgacccgag attacgtcct tcaggcgatc tcgcggggac
    14941 tcgacgagat cgcgttcacc gaccacgtgc ccctctggtt cctccccggc gacgacccgg
    15001 agcccggcct cgcgatgacg cgggccgagc tgccggagta cgtcgaagag gtgctcgcgc
    15061 tgcgcgagga gttccgcggc cggatcgacg tc
//
LOCUS       DAOONL010000082        15060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4215_length_15060_cov_15.375541, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000082 DAOONL010000000
VERSION     DAOONL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_15060_cov_15.375541"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1775
                     /locus_tag="PLP50_11985"
     CDS             3..1775
                     /locus_tag="PLP50_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52310.1"
                     /translation="MIDARARRDRIAQWAFDARPVLGRFHLWLEDVEESRVEGEAGLD
                     LSFAGTSLEKCLIMTAGVTALGTRLFGRYGEAKGLDAHGANHVKKDADAVSAYAMSEG
                     LWYLTRSLPENHAIMVSLGEGLMPKGGETPDMGSNPLLGFGRVYARPSVAKWLDARVH
                     RFINDASYGWRTFWSELMAARITVWGAAIDTLENTSRFAKGAPTGPTTVLHLFDQPLR
                     IARPYEGYTGAMTLPKEVFETAADSSILLDFLTPRSLVLDAIRKTYPGIEPGDVHVWT
                     LAGPTRVKRIGRLWDEWRALGAHLVEDGWSLPSGLGAFTDSGTYAPTYRVGPFTGPDG
                     RTHLFLTDGYAASAEALQAASLDPILGNRTSMCVLTSKFEVSYDRERRILFLDPAAPD
                     FPQRLSEILEREVAPKDCLAYAGILTNARDAAMPLGKRTVTADDFFQKKEWHVLSLMG
                     SMLPEPYTGLAGVEQVKQGVYRVTTRAASERGVLDVTLTLRLMESFEQTRLVFSPLLD
                     RFYRGQDWKTRPVKVSDSGRIRNELQTLASEALEYFGENGIRVHFDRVDDAVLAPDKK
                     ALIREVLAWYKEHHPIWFGWLELA"
     gene            1772..3445
                     /locus_tag="PLP50_11990"
     CDS             1772..3445
                     /locus_tag="PLP50_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPA52311.1"
                     /translation="MSAGAGLALIPVELPWTKTEVVEGTTVDELFDAGPTEGRDVVAA
                     CVNDRVVALDFPIECPSRVAPVDASSREGEEVVRRTATLLLHGVARKLFPKTRLHVGQ
                     SLRGGYHYGVSGEYPPPAELAAALDEAFHREADAGRQVVRQEVSLDAALHLFEQEGEE
                     LKVRLLRIWPSHRVPLVSCLGFTDVRHGPYALSVRPCRSFHVEAHEQGLLLVFDGPAG
                     QAEKLGGGTVLFNAYKETKRWNELVGVGTVPDLNDQCLGDRIKHVIRIAEGQHEKKIA
                     ELADRIAARRDELRVVCIAGPSSSGKTTFVKRLSIQLEVNGIVPRTLSLDDYYVDREK
                     TPLDADGDLDFEALEAIDLPLFLAQLKELVEGREVLTPVYDFKAGLRKPESAWRPRRL
                     GGNEVLLVEGIHGLNPRLTESVGESRKFRIFINALTQLCIDDHNRIRTSDARLLRRIV
                     RDRRYRGYSAETTILRWPKVRAGEEKHIFPFQERCDAMFNSALAYETAVLKSFADRYL
                     LEVPPVSPAQARGFQLRRFLELFVPVWPDDVPATSILREFIGGSGFSYK"
     gene            complement(3543..5951)
                     /locus_tag="PLP50_11995"
     CDS             complement(3543..5951)
                     /locus_tag="PLP50_11995"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPA52312.1"
                     /translation="MSFSSRQVTLTGLSAFATLLAAAPAAAEGTARSSVGAVWWLAPI
                     SSVVALAMAWVLFRMMRGADPGNERMQEIARYVREGAFAYLRRQYRVVGLVFGVMVVL
                     LAILAFMGIQNPFVPVAFLTGGFFSGLCGFIGMNTATLASSRTAEGCRHSLNRGLQVA
                     FRSGAVMGLIVVGFGLLDICIWYFVLDRWVYTPEHMASGLKALGLVFVHAGTTPHDKL
                     VEITVTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIA
                     DNVGDNVGDVAGMGADLYESYCGSILSTAALGAAIPALGESGVLAPMIVAGIGTILSI
                     IGIFMVRTKEEATQKNLLRSLLIGTLGASALIVPAVAILAWLGYVPEGVWISVIAGLF
                     AGVVIGQATEYYTSDEYSPTKGIAKQCTMGPATAVIDGVAVGMYSTGIPVVTIVVAIL
                     VSYWAPGGFTDVSYGLYGIGFAAVGMLATLGITLATDAFGPIADNAGGNAEMSHLPAE
                     VRQRTDALDSLGNTTAATGKGFAIGSAALTAMALLAGYLEEIRVWLGRMAAEAPNALS
                     IQGPIAFFKGKAAEAPAEVLEAAKAAGKTLVGTADAKMADFMAAYDVTLMNPSVLCGL
                     FLGAMMVLVFSAMTMKAVGRAAGSMVEEVRRQFKEFPGIMEGTQKPDYARCVAISTAG
                     AQREMIVPSLLSIAVPIAVGLVFGVAGVMGLLVGGLSCGFVFAIMLNNAGGAWDNAKK
                     HIERGAYGGKGSDAHKAGVTGDTIGDPFKDTTGPSLNILLKLMSMVSVVFSGLVVAFG
                     GKLF"
     gene            6095..6751
                     /locus_tag="PLP50_12000"
     CDS             6095..6751
                     /locus_tag="PLP50_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPA52313.1"
                     /translation="MSSPAARPRPLTKGEEIANSVTHGVGLLASVVAFPFLVVAAARR
                     WGASEVVGASVFAATLVALYLFSTLYHALRPSRAKQVLQKLDHTAIYLLIAGSYTPFT
                     LGVLRGPWGWTLFSLVWAIAGTGIALEFALGKKVHKVAVALYVVMGWLVVVAAKPLLA
                     SVPWPGLAWLAAGGLLYTGGVAFYAARRVPYAHTVWHLFVLAGSACHFVAIWGWSGRA
                     "
     gene            6800..7051
                     /locus_tag="PLP50_12005"
     CDS             6800..7051
                     /locus_tag="PLP50_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPA52314.1"
                     /translation="MSILWTILIGFVIGVIAKFLHPGKENMGLVLTTALGIAGSLVAT
                     FLGRLVGFYGEGEVAGFLMAVVGAIVLLVVYTLVRGKAK"
     gene            7196..7597
                     /locus_tag="PLP50_12010"
     CDS             7196..7597
                     /locus_tag="PLP50_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52315.1"
                     /translation="MSGRAHGTPPVRATAEQSAEVFAALRSILSAHARELKVTADGPK
                     GYSLNTPYSGRWKKELFFGGVQVTKNYVSFHVFPVYMYPDLLDGATPGLKARMQGKSC
                     FNFRAPEPELFAELAALVAAGLARMKAEKDF"
     gene            7731..7949
                     /locus_tag="PLP50_12015"
     CDS             7731..7949
                     /locus_tag="PLP50_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52316.1"
                     /translation="MKAKVIAGLLLVALGVVALAYRTFTYTKRTHEAKVLGLELSLKE
                     KETVEVPVWAGAGAVVVGALLLVAGRKK"
     gene            7959..8279
                     /locus_tag="PLP50_12020"
     CDS             7959..8279
                     /locus_tag="PLP50_12020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPA52317.1"
                     /translation="MESAPVTVLIRYQASQGKEGDAVTLLSELIATVVREEPDCLGIE
                     LFTYDADPGRILLNETWTSREAYLGPHFQTPHLKAFIEAASAVFADAPDISFWELRAA
                     VRRD"
     gene            8303..8647
                     /locus_tag="PLP50_12025"
     CDS             8303..8647
                     /locus_tag="PLP50_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52318.1"
                     /translation="MSETVELWGFLSDDVEAAREETERLLGVTFRLHESETIGPYYFA
                     ELDEPESELTLRPNLDPDFDEDVGDPDDAFAEPDFPDFGVLLYVERRSGGEAGGGRLA
                     ALGGAAQLLLVE"
     gene            8695..9177
                     /locus_tag="PLP50_12030"
     CDS             8695..9177
                     /locus_tag="PLP50_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPA52319.1"
                     /translation="MSGDPSVTSELVRDFVAQRRLALAGVSRSGRKFGNLVLRELKAR
                     GYEVIPVHPEAGELQGLPCARSLAELKGRVDGVVVVTPPSETAKLVGEAADAGIGRVW
                     LQQGAESEEAIRAARERGLALVHGHCLLMFLTGAGGIHRLHGFLWRLLGKLPAEAART
                     "
     gene            9223..9612
                     /locus_tag="PLP50_12035"
     CDS             9223..9612
                     /locus_tag="PLP50_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52320.1"
                     /translation="MDPSFTSFVLAFSCGVVAFGIASVALAAVFVQGRFETSRLFLPA
                     AGIVACGLLAGFLAIVASSGGLPWGAVGLPAAASLGLSVLGLSVARGMPPLAGYGRID
                     TWLFATCLAGSALLAVILLVLLARGGA"
     gene            9683..10201
                     /locus_tag="PLP50_12040"
     CDS             9683..10201
                     /locus_tag="PLP50_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52321.1"
                     /translation="MKRSLLSPLALPALLTAFVVATPSYAQDATVSSAEDKAQALALV
                     QQLEEDPLASGADNSRQWLTNFIVNAPDLFISTCPELLGALNGQGKNGAPELVGQLYF
                     GNAAWAIQNPDKASDREAMYAGGIESTLRLYASLLKSKPRWRWPILDKLRSRAKDGTL
                     IEYVRENIASCQ"
     gene            10215..10667
                     /locus_tag="PLP50_12045"
     CDS             10215..10667
                     /locus_tag="PLP50_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52322.1"
                     /translation="MSRLLRLPLVVVLLAAAGCGGSPETRSFPTRAEAGPAVAAWVPA
                     EATDLRLATEERSGAFWLRFRLSKSGREALEPGLVRIPDTQVATLTFRAPKGVDWWFR
                     GLVQVVPARETTVVAADVHSGNGTAVPRGTFVAFDRASDAVYAWAAGR"
     gene            10743..11117
                     /locus_tag="PLP50_12050"
     CDS             10743..11117
                     /locus_tag="PLP50_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52323.1"
                     /translation="MLRIAGVVFALVGLGAGVFLLAGSFGLTPNAPGLSAWVLFPACA
                     FGGLVLAALGSRTGALPGLLGVAGGALLLLALAAAVGLLLSAAGYAETRNGSGSLWYL
                     LGVAGLAGAAWLLAPEGPGGKA"
     gene            complement(11187..12365)
                     /locus_tag="PLP50_12055"
     CDS             complement(11187..12365)
                     /locus_tag="PLP50_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52324.1"
                     /translation="MLSIPLKYNVRNLFVRKVTTSLTVLGIALVVAVFLCVMALGEGL
                     ATVFTASGSEKNVLVLRQNSQSELQSGISRDQAPLVKALPGIEKDADGKPLVSAELVV
                     VLNLDKRDGGSSNVTLRGVEEKGMALRPGMRITEGRTFVPGTSEVIVAESVARRFKGA
                     EVGQEIRFGAYRWKVVGHFDAGGTAPDSEIWTDTEGMANTFQRGGYSSILARTTDRAA
                     RDQLVAALAGDARLALDGKVERAYYDEQTATAAPIRFLGFFVGVVMAIGACFGAMNTM
                     YAAVSARTREIATLRALGFSRFSILVSFVLESIALALLGGVAGCALAFLLVKLALSGV
                     TGTTNFATFAEVVFAFRLTPQLLATGIVFSLVMGFFGGLFPAGRAAFTKITAALRQVG
                     "
     gene            complement(12490..13647)
                     /locus_tag="PLP50_12060"
     CDS             complement(12490..13647)
                     /locus_tag="PLP50_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52325.1"
                     /translation="MKFVPYVLKSLFRKRTRSVLTILSIVLPFFVICVLGTLLATLDA
                     DPSGGKGMFRLVVRHKVSLANFIPEAYRERIAQLPGVEEVTILNWFGGTYKDNRPENM
                     FARFGCEPEKLVKIFDEVTIVAGTAAEWASDRKGALVGERLMKRYGWKIGDSFVLKGD
                     IFPIDLELTVRAVFRGSDETGVYFDRKAVEEAVPWAKGQVGTFWIKAASAEAVQSLPR
                     QIDALFENSSFPTKTETEKEFQNGFVSMLGNVKAVVTGISTAIALVILLISANTMAMA
                     ARERVTEIAVLRTLGFGKSLILTLILAESLLLSATGAFVGLGLFRLSFPALKEGLLSS
                     PMAGFAAGMQLFPEVLVTGIGVTLLVGLVAGIVPAIRSARRSIPDGLRQVG"
     gene            complement(13644..14804)
                     /locus_tag="PLP50_12065"
     CDS             complement(13644..14804)
                     /locus_tag="PLP50_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52326.1"
                     /translation="MKILPLVLKNLLRKKTRSALTIASIVLPLFVICILGTLLRGLET
                     DPSGGKGMYRLIVRHKVSITNWIPGAYDPKIRNVPGVTEAVRMQWFGGAYIDQSAKNQ
                     FQRFSVTDVDRLLRVYDEATIVEGSEADWVGDRTGLLVGRQLMRKYGWRLGQKVTFKG
                     DIYPVTLELTIRAVFEAPDESGVYFHHKGIEEAVPYAKGQVGWYWLKTASVADTERVP
                     RLIDAMFENSPEPTRTETEKQFQAMWVSMIGNVKAVVVGISTAIALVILLIAANTMAM
                     AARERVTEIAVLRVLGFTKEKVLGLVLGESFALSMIGGGLGIGLFVLLLPGLREGIVN
                     SPMASFAAGIRLFPEVLVLGFGMTFAVGLFAGLVPAIRSAQRPITEGLRQVA"
     gene            complement(14809..>15060)
                     /locus_tag="PLP50_12070"
     CDS             complement(14809..>15060)
                     /locus_tag="PLP50_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52327.1"
                     /translation="LLVADEPTGDLDAKSGEEILVLLERLHAEFGKTIVMVTHDPKAA
                     ARAHRLVHLDKGVLKEDVRAKGEKGEIGVAAAAAIAAGK"
BASE COUNT         2219 a         5259 c         5324 g         2258 t
ORIGIN      
        1 tcatgatcga cgcacgcgca cgccgtgacc ggatcgctca gtgggccttc gacgcccgtc
       61 ccgtgctcgg ccgcttccac ctctggctcg aggacgtcga ggagagccgc gtcgaagggg
      121 aggccggcct ggacctctcg ttcgcgggga cctcgctcga gaagtgcctc atcatgacgg
      181 ccggcgtcac ggcgctcggc actcgcctct tcggccgcta cggcgaggcg aaggggctgg
      241 acgcccacgg ggcgaaccac gtcaagaagg acgccgacgc cgtgtcggcc tacgcgatga
      301 gcgagggact ctggtacctg acgcggtcgc ttccggagaa ccacgccatc atggtgagcc
      361 ttggcgaggg gctgatgccg aagggaggcg agacgcccga catgggctcg aacccgctcc
      421 tcggcttcgg gcgcgtctac gcccgcccgt cggtcgcgaa gtggctcgac gcgcgcgtcc
      481 accgcttcat caacgacgct tcgtacggct ggcggacgtt ctggagcgag ctgatggcgg
      541 cgcgcatcac cgtgtggggc gcggcgatcg acacgctgga gaacacctcc cgcttcgcga
      601 agggagcgcc gacggggccg acgacggtcc tccacctctt cgaccagccg ctccggatcg
      661 cacgccccta cgaggggtac accggcgcga tgacgctccc gaaggaggtc ttcgagacgg
      721 cggccgactc ctcgatcctc ctcgacttcc tcacgccgcg ttccctcgtc ctggacgcga
      781 tccggaagac ctaccccggc atcgagccgg gcgacgtgca cgtctggacg ctcgcggggc
      841 cgacgcgcgt gaagcggatc ggcaggctct gggacgaatg gagggcgctc ggcgcccacc
      901 tcgtcgagga cggctggtcg ctcccgagcg gcctcggcgc cttcaccgac tcggggacct
      961 acgctccgac atatcgggtc gggccgttca cggggccgga cggacggacg cacctcttcc
     1021 tgacggacgg ctacgcggcc tccgccgagg cgctccaggc cgcgagcctc gacccgatcc
     1081 tcgggaaccg gacctcgatg tgcgtcctga cgtcgaagtt cgaggtctcc tacgatcggg
     1141 aacggcggat actcttcctc gaccccgccg ccccggactt cccgcagcgc ctttccgaga
     1201 tcctggagcg cgaggtggcg ccgaaggact gcctcgccta cgccggcatc ctgacgaacg
     1261 cccgcgacgc ggcgatgcct ctcggaaagc ggaccgtcac ggccgacgac ttcttccaga
     1321 agaaggagtg gcacgtcctc tcgctgatgg ggagcatgct ccccgagccc tacaccggcc
     1381 tcgccggcgt cgagcaggtg aagcaggggg tctatcgcgt cacgacgcgc gcggcctcgg
     1441 aacgcggggt cctggacgtg acgctgacgc tccgcctgat ggagtcgttc gagcagacgc
     1501 gcctcgtctt ctcgccgctg ctcgaccgct tctaccgggg tcaggactgg aagacgcggc
     1561 cggtgaaggt ctccgactcg ggccggatcc gcaacgagct ccagacgctc gcgtcggagg
     1621 cgctcgagta cttcggcgag aacggcatcc gcgtccactt cgaccgggtc gacgacgccg
     1681 tcctcgcgcc ggacaagaag gccctcatcc gggaggtcct cgcctggtac aaggagcacc
     1741 atccgatctg gttcggctgg ctcgagctcg catgagcgcg ggcgccggcc tcgcgctgat
     1801 ccccgtcgag ctcccgtgga cgaagaccga ggtcgtcgag gggacgacgg tcgacgagct
     1861 gttcgacgcg ggcccgacgg agggccggga cgtcgtcgcg gcgtgcgtca acgaccgggt
     1921 cgtcgccctc gacttcccga tcgagtgccc gagccgcgtc gcgccggtcg acgcctcctc
     1981 gagggaaggg gaggaggtcg tccgtcgcac ggccacgctc cttctccacg gcgtcgcccg
     2041 gaagctcttc ccgaagacgc gcctccacgt cggccagagc ctccgcgggg gttaccacta
     2101 cggggtctcc ggcgagtacc cgccgcccgc cgagctcgcc gccgccctcg acgaggcgtt
     2161 ccaccgggag gccgacgcgg gccgccaggt cgtccggcag gaggtctcgc tcgacgcggc
     2221 gctccacctc ttcgagcagg agggggagga gctgaaggtc cgcctcctcc ggatctggcc
     2281 gtcgcaccgc gtccccctcg tctcctgcct cggtttcacc gacgtgaggc atggccccta
     2341 cgccctttcc gtacggccct gccggagctt ccacgtcgag gcgcacgagc aggggctcct
     2401 cctcgtcttc gacggtcccg cggggcaggc ggagaagctc ggggggggga cggtcctctt
     2461 caacgcctac aaggagacga agcgctggaa cgagctcgtc ggcgtcggga cggtgccgga
     2521 cctgaacgac cagtgcctcg gcgaccggat caagcacgtc atccggatcg cggaggggca
     2581 gcacgagaag aagatcgccg agctcgcgga ccggatcgcg gcgcgacgcg acgagctccg
     2641 cgtcgtctgc atcgcgggcc cctcgtcctc ggggaagacg acgttcgtca agcggctctc
     2701 gatccagctc gaggtcaacg gcatcgtgcc gcggaccctt tcgctcgacg actactacgt
     2761 cgaccgggag aagacgccgc tcgacgccga cggcgacctc gacttcgagg ccctcgaggc
     2821 gatcgacctc ccgctcttcc tcgcccagct gaaggagctc gtcgaggggc gcgaggtcct
     2881 gaccccggtc tacgacttca aggccgggct ccggaagccc gagagcgcct ggcgcccccg
     2941 ccgcctcggc gggaacgagg tcctcctcgt cgaggggatc cacggcctga acccgcgcct
     3001 gaccgagtcg gtcggggaga gccgcaagtt ccggatcttc atcaacgcgc tgacgcagct
     3061 ctgcatcgac gaccacaacc ggatccggac gagcgacgcg cgcctcctcc gccgcatcgt
     3121 ccgcgaccga aggtaccggg gctactcggc cgagacgacg atcctccgct ggccgaaggt
     3181 gcgcgccggc gaggagaagc acatcttccc gttccaggag cgctgcgacg cgatgttcaa
     3241 ctcggccctc gcctacgaga cggccgtcct gaagagcttc gccgaccgct accttctcga
     3301 ggtcccgccg gtctcgccgg cccaggcccg ggggttccag ctccgtcgtt tcctcgagct
     3361 cttcgtcccg gtctggccgg acgacgtccc ggcgacgagc atcctccggg agttcatcgg
     3421 cgggagcggc ttctcgtaca agtaggccgc gctccgggcc cgccggagaa ggcgacgggg
     3481 gcgcgtccga cggcgccccc gtcctccttc gagcgcgggg ccccgggccc cgccgaccgg
     3541 gactagaaga gcttcccgcc gaaggcgacg acgagccccg agaagacgac cgagaccatg
     3601 ctcatcagct tgagcaggat gttcagcgac gggccggtcg tgtccttgaa ggggtcgccg
     3661 atcgtgtcgc ccgtgacgcc cgccttgtgg gcgtcggagc ccttcccgcc gtaggcgccc
     3721 cgctcgatgt gcttcttggc gttgtcccag gcgccgccgg cgttgttcag catgatcgcg
     3781 aagacgaacc cgcaggagag cccgccgacg aggagcccca tgacgccggc gacgccgaag
     3841 acgaggccga cggcgatcgg gacggcgatg gagaggagcg acgggacgat catctcgcgc
     3901 tgcgcgccgg ccgtggagat ggcgacgcag cgggcgtagt cgggcttctg ggtcccctcc
     3961 atgatcccgg ggaactcctt gaactgccgg cggacctcct cgaccatcga ccctgcggcg
     4021 cggccgaccg ccttcatcgt catggcggag aagacgagga ccatcatggc gccgaggaag
     4081 agcccgcaga ggacggaggg gttcatcagc gtgacgtcgt aggcggccat gaagtcggcc
     4141 atcttcgcgt cggcggtccc gacgagcgtc ttcccggccg ccttcgcggc ctcgaggacc
     4201 tcggcagggg cctcggcggc cttccccttg aagaacgcga tcgggccctg gatcgagagg
     4261 gcgttcggcg cctcggcggc catgcggccg agccacacgc ggatctcctc gaggtagccg
     4321 gcgaggagcg ccatcgccgt gagcgccgcc gagccgatcg cgaagccctt gccggtggcg
     4381 gcggtcgtgt tcccgagcga gtcgagcgcg tcggtccgct ggcggacctc ggccggaagg
     4441 tgcgacatct cggcgttccc gccggcgttg tcggcgatcg ggccgaacgc gtcggtggcg
     4501 agcgtgatcc cgagcgtcgc gagcatgccg accgccgcga agccgattcc gtagaggccg
     4561 tacgagacgt ccgtgaaccc gccgggcgcc cagtacgaga cgaggatcgc gaccacgatc
     4621 gtcacgaccg ggatgcccgt ggagtacatg ccgaccgcga cgccgtcgat gaccgccgtc
     4681 gccgggccca tcgtgcactg cttcgcgatc cccttcgtcg gggaatactc gtcggaggtg
     4741 tagtactcgg tcgcctgtcc gatgacgacc ccggcgaaga ggccggcgat gaccgagatc
     4801 cagaccccct ccgggacgta gccgagccag gcgaggatcg ccacggccgg gacgatgagg
     4861 gcgctcgcgc cgagcgtgcc gatgaggagc gagcggagga ggttcttctg tgtcgcctcc
     4921 tccttcgtcc tcaccatgaa gatgccgatg atcgagagaa tcgtcccgat ccccgcgacg
     4981 atcatcgggg cgaggacgcc cgactcgccg agcgccggga tcgcggcgcc gagcgcggcg
     5041 gtggagagga tcgagccgca gtacgactcg tagaggtcgg cgcccatgcc ggccacgtcg
     5101 ccgacgttgt cgccgacgtt gtcggcgatc gtcgccgggt tgcgcgggtc gtcctcgggg
     5161 atgccggcct cgaccttgcc gacgaggtcg gcgccgacgt ccgccgcctt cgtgtagatg
     5221 ccgccgccga cgcgggcgaa gagcgcctgc gtggaggcgc ccatgccgaa cgtgagcatc
     5281 gtgacggtga tctcgacgag cttgtcgtgc ggggtcgtac cggcgtggac gaacacgagg
     5341 ccgagggcct tcaggccgct cgccatgtgc tccggcgtgt agacccaccg gtcgaggacg
     5401 aagtaccaga tgcagatgtc gagcagcccg aagccgacga cgatcagccc catgacggcg
     5461 ccgctgcgga acgcgacctg caggccacgg ttcagcgagt ggcggcaccc ctcggccgtc
     5521 cgggaggagg cgagcgtcgc cgtgttcatc ccgatgaagc cgcagaggcc cgagaagaag
     5581 ccgcccgtca ggaaggcgac cgggacgaac gggttctgga tccccatgaa ggcgaggatc
     5641 gcgaggagga cgaccatgac gccgaagacg aggccgacga cccggtactg ccggcggagg
     5701 taggcgaagg cgccctcccg gacgtagcgg gcgatctcct gcatccgctc gttccccggg
     5761 tccgcccccc gcatcatacg gaagaggacc cacgccatgg cgagcgcgac gaccgaggag
     5821 atcggcgcga gccaccagac ggccccgacg ctcgatcgcg cggtgccctc ggcggccgcc
     5881 ggcgcggcgg cgagcagtgt ggcgaaggcc gagagcccgg tgagtgtgac ctgcctcgac
     5941 gaaaacgaca ttccgcgtcc ccccttgcgc tggtttcgtg ctccccccgg aagcctccgt
     6001 gaaaaccgaa acgcgtgatt ctctgccggg cgggaccgga tttcaaagcc attcgaccgg
     6061 cgggccggcc ccgggcgggt tagcatccgg cctcatgtcg agccccgccg cccgcccgcg
     6121 ccccctgacg aagggggagg agatcgccaa cagcgtgacg cacggcgtcg gcctcctcgc
     6181 gagcgtcgtc gctttcccgt tcctcgtcgt ggccgcggcc cgccgctggg gggcgtccga
     6241 ggtcgtcggg gcctccgtct tcgcggcgac cctcgtcgcc ctctacctct tctcgacgct
     6301 ctaccacgcc ctgcggccgt cccgggccaa gcaggtgctg cagaagctcg accacacggc
     6361 gatctacctc ctgatcgccg gctcgtatac gccgttcacg ctcggggtcc tgcggggtcc
     6421 gtggggatgg accctcttct ccctcgtctg ggcgatcgcc gggaccggga tcgcgctcga
     6481 gttcgcgctc gggaagaagg tccacaaggt cgcggtcgcg ctctacgtcg tcatggggtg
     6541 gctcgtcgtc gtagcggcga agccgctcct cgcctccgtc ccgtggccgg gcctcgcgtg
     6601 gctggcggcc ggcgggctcc tctacacggg cggcgtcgcc ttctacgccg cccggagggt
     6661 cccgtacgcg cacaccgtct ggcacctgtt cgtcctcgcg gggagcgcct gccacttcgt
     6721 cgcgatctgg ggttggtcgg gccgagcctg acgccgccgc gttgcggcgc gccgcccgga
     6781 ggaagacaat cgctccatgt tgagcatcct ctggaccatc ctgatcggct tcgtcatcgg
     6841 cgtcatcgcg aagttccttc accccggcaa ggagaacatg ggcctcgtcc tgacgacggc
     6901 cctcggcatc gcgggctcgc tcgtcgccac gttcctcggc cggctcgtcg gcttctacgg
     6961 agagggcgag gtggcgggct tcctgatggc cgtcgtcggc gcgatcgtcc tcctcgtcgt
     7021 ctacacgctc gtccggggga aggcgaagta gtcccggctc ccgccctgac gggcgtccct
     7081 ggtctcgtcg aggtggcgcc gtccctccct cccccgccgg tccgcccggt ccgtcgatgc
     7141 gatctcgccg gaggctggta gcctgctccc gcaacgagcg ccgcaggagg tcgacatgtc
     7201 cgggagagcc cacggcaccc caccggtccg cgccaccgcc gagcagtccg ccgaggtctt
     7261 cgcggcgctc cggtcgatcc tctcggccca cgcgcgggag ctgaaggtga ccgccgacgg
     7321 acccaagggc tactcgctga acacccccta ctccgggcgg tggaagaagg agctcttctt
     7381 cggcggcgtc caggtcacga agaactacgt ctccttccac gtcttcccgg tctacatgta
     7441 cccggacctg ctcgacgggg ccacgcccgg gctgaaggcg cgaatgcagg ggaagtcgtg
     7501 cttcaacttc cgcgcgcccg agccggagct cttcgccgag ctcgccgcgc tcgtcgcggc
     7561 cgggctcgcg cggatgaagg ccgagaagga cttctgaccc ggccgacccg gaacgggatg
     7621 ccggaccgat tcgtcccggt ctatcctccg cgcgacgcaa cggccgccgg acgacggagc
     7681 cttcccggtc cctcggggag gtgggtccgc cggcgtagca ggaggtcggg atgaaggcga
     7741 aggtgatcgc ggggctcctc ctcgtcgcgc tgggggtcgt ggccctcgcg tacaggacgt
     7801 tcacgtacac gaagaggacg cacgaagcga aggtcctcgg cctcgagctc tcgctgaagg
     7861 agaaggagac ggtcgaggtc cccgtctggg ccggcgccgg agcggtcgtc gtgggcgccc
     7921 tgctcctcgt cgcggggcgg aagaagtagg aggggccgat ggagagcgcg cccgtgaccg
     7981 tcctgattcg ctaccaggcc agccagggga aggagggcga cgccgtgacc ctcctctcgg
     8041 agctgatcgc gaccgtcgtg agggaggagc cggactgtct cgggatcgag ctcttcacct
     8101 acgacgccga tccggggcgg atcctcctga acgagacctg gacctcgcgg gaggcgtatc
     8161 tcggtccgca tttccagacg ccgcacctga aggcgttcat cgaggcggcg tcggccgtgt
     8221 tcgcggacgc gcccgacatc tccttctggg agctgcgggc cgcggtgcgg cgcgactgaa
     8281 ggcgctccgc gcggagattc tcgtgagcga aaccgtcgag ctctgggggt tcctatccga
     8341 cgacgtcgag gcggcccgcg aggagaccga gcggctcctc ggggtgacgt tccggctgca
     8401 cgagagcgaa acgatcgggc cgtactactt cgccgagctg gacgagccgg aatcggagct
     8461 cacgctccgc ccgaacctcg acccggactt cgacgaggac gtcggcgacc cggacgacgc
     8521 gtttgccgag cccgacttcc ccgacttcgg cgtcctcctc tacgtggagc ggcgctccgg
     8581 gggcgaggcg ggcggggggc gtctcgccgc gctcggcgga gcggcgcagc tcctcctcgt
     8641 ggagtagcgt gagacgaggg ccgtccgccc cgagggcggg cgcggaggtg cttcttgagc
     8701 ggtgacccgt ccgtgacctc ggagctggtc cgggacttcg tcgcgcagcg gcgactggcg
     8761 ctggcgggag tctcgaggtc gggccggaag ttcgggaacc tggtcctccg cgagctgaag
     8821 gcccgcggct acgaggtgat cccggtccat cccgaggccg gggagctgca gggcctcccg
     8881 tgcgcgagga gcctcgcgga gctgaaggga cgggtcgacg gggtcgtcgt cgtcacgccc
     8941 ccgtccgaga ccgcgaagct cgtcggggag gccgccgacg ccggcatcgg acgcgtctgg
     9001 ctccagcagg gcgccgagtc ggaagaggcg atccgggcgg cgcgcgagcg gggcctcgcc
     9061 ctcgtgcacg ggcactgcct cctgatgttc ctgaccggcg cgggcgggat ccatcggctc
     9121 cacggctttc tctggcgcct cctcgggaag ctcccggccg aggcggcccg aacgtgacgg
     9181 gagacggccg gcgggaaggc cggacagcct gaggggggga acgtggaccc gtcgttcacc
     9241 tcgttcgtcc tcgcgttctc gtgcggcgtc gtcgccttcg gaatcgcgtc ggtggccctg
     9301 gccgccgtct tcgtccaggg gcgcttcgag acgagccggc tcttcctccc cgccgcagga
     9361 atcgtcgcgt gcgggctcct ggccggcttc ctcgcgatcg tcgcctcgtc cggggggctt
     9421 ccctggggcg ccgtcggcct gccggcggcc gcgtcactcg gcctctcggt cctcggcctc
     9481 tcggtcgcgc gcggaatgcc cccccttgcg ggctacggac gaatcgacac ctggctcttc
     9541 gcgacgtgcc tggccggctc cgccctgctc gccgttatcc tcctggtcct tctggcgcgc
     9601 ggcggagcct gaggggatgc cgagaggccg attcaggacc ggggatgatc tgaagacgtt
     9661 tttagggcat agtaacgagc ccatgaagag gtctctcctt tcgccgctcg cgctgccggc
     9721 cctcctgact gcattcgtcg tcgccacgcc gtcgtacgcg caggatgcaa ccgtctcctc
     9781 cgcggaagac aaggcgcagg ctctcgccct cgtccagcag ctcgaagagg accctctcgc
     9841 ctcgggcgcg gacaacagcc gccagtggtt gacgaacttc atcgtcaacg cgccggacct
     9901 cttcatctcg acctgtcccg agctcctcgg cgcgctgaac ggccagggga agaacggcgc
     9961 ccccgagctc gtcgggcagc tctacttcgg caacgcggcc tgggcgatcc agaaccccga
    10021 caaggcgtcc gaccgcgagg cgatgtacgc cggcgggatc gagagcacgc tccggctcta
    10081 cgcgagcctc ttgaagtcga agccgcgctg gcgctggccg atcctcgaca agctccgttc
    10141 ccgcgcaaag gacggcacgc tgatcgagta cgtccgcgag aacatcgcct cctgccagta
    10201 gcgaacgggg cgacgtgagc cgcctccttc gactcccgct cgtcgtcgtc ctcctcgcag
    10261 cggcgggctg cggcggctcg ccggagacgc gctcgttccc cacgcgggcc gaggcggggc
    10321 cggccgtcgc cgcgtgggtc ccggccgaag cgaccgacct gcggttggcg acggaggagc
    10381 ggagcggcgc gttctggctc cggttccgcc tttcgaagtc cgggcgggag gccctcgagc
    10441 cgggtctggt ccgtatcccc gacacccagg tggcgacgct cacgttccgg gcgccgaagg
    10501 gggtcgactg gtggttccgc ggcctcgtcc aggtcgtccc ggcccgggag acgaccgtcg
    10561 tcgcggcgga cgtccactcg gggaacggaa cggcggtgcc gcgggggacc ttcgtcgcct
    10621 tcgaccgggc ctcggacgcc gtctacgcgt gggcggccgg gcgctaggag ctctccgcgc
    10681 ccggtccggg cgcgccggag cgggacgcga acgataatcc ccggagctgg aggtttcaga
    10741 gcgtgctgag aatcgcggga gtcgtcttcg ccctcgtcgg actcggcgcc ggcgtctttc
    10801 tcctcgcggg ctcgttcggg ctgacgccga acgctccggg cctttccgcc tgggtcctct
    10861 tcccggcctg cgcgttcggc gggctcgtcc tcgcggcgct cggctcgcga acgggggcgc
    10921 tccccggcct cctcggcgtg gccggcgggg cgctcctcct cctcgcgctc gccgcggccg
    10981 tcggcctgct cctctcggcg gcgggctatg ccgagacgcg gaacggaagc ggctcgctct
    11041 ggtacctcct cggcgtcgcg ggcctcgcgg gggccgcgtg gctgctggcg ccggagggtc
    11101 ccgggggaaa ggcctgacgc gccgcgggcg gcgccggagc gccgcccgcg acctcgaggg
    11161 cttcccggga gcgccggtcg ccggcctcag cccacctgcc ggagagccgc ggtgatcttc
    11221 gtgaaggccg cgcgccccgc cgggaagaga ccgccgaaga agcccatgac gagcgagaag
    11281 acgattcccg tcgcgaggag ctgcggcgtc aggcggaacg cgaagacgac ctcggcgaac
    11341 gtcgcgaagt tcgtcgtccc ggtgacgccc gagagcgcga gcttcacgag gaggaacgcg
    11401 agcgcgcagc cggcgacgcc gccgaggagg gcgagcgcga tcgactcgag gacgaaggag
    11461 acgaggatcg agaagcgcga gaagccgagc gcgcgcagcg tcgcgatctc gcgcgtgcgg
    11521 gccgagacgg ctgcgtacat cgtgttcatc gcgccgaagc aggccccgat ggccatgacg
    11581 acgccgacga agaacccgag gaacctgatc ggcgcggccg tggccgtctg ctcgtcgtag
    11641 taggcgcgtt cgaccttgcc gtcgagcgcc aggcgcgcgt cgccggcgag cgcggcgacg
    11701 agctggtcgc gcgcggcgcg gtcggtcgta cgggcgagga tcgaggagta gccgccccgc
    11761 tggaacgtgt tcgccatccc ctcggtgtcg gtccagatct cgctgtcggg ggcggtcccg
    11821 ccggcgtcga agtgcccgac gaccttccag cggtaggcgc cgaagcggat ctcctggccg
    11881 acctcggccc ccttgaacct ccgggcgacg ctctcggcga cgatcacctc agaggttccc
    11941 gggacgaacg tccgcccctc ggtgatccgc atcccggggc ggagcgccat ccccttctcc
    12001 tcgacgccgc ggagcgtgac gttcgaggag ccgccgtccc tcttgtcgag gttcaggacg
    12061 acgacgagct cggccgagac gagcggcttg ccgtcggcgt ccttctcgat gccggggagc
    12121 gccttcacga gcggcgcctg gtcgcgcgag atccccgact ggagctccga ctgcgagttc
    12181 tggcggagga cgaggacgtt cttctccgac ccggaggccg tgaagacggt cgcgagcccc
    12241 tcgccgagcg ccatcacgca gaggaagacg gcgacgacga gcgcgatgcc gaggacggtg
    12301 agcgacgtcg tcaccttcct cacgaagagg ttccggacgt tgtacttcag cgggatggag
    12361 agcacgttcg actcctgcct ctagcgtgtc cggcgttcgg gtgtgagcgc ggagagggtc
    12421 cgggagaacc cggcgttcgg gttctcccgg gagcatttca gcgggatgga gagcacgttc
    12481 gactcctctc tagccgacct ggcggaggcc gtcggggatg gagcggcggg cggagcggat
    12541 cgccgggacg atcccggcga cgaggccgac gaggagcgtc accccgatcc ccgtcacgag
    12601 gacctcgggg aagagctgca tccccgcggc gaagccggcc atcggcgagc tgaggagccc
    12661 ttccttcagc gcggggaacg agagccggaa gaggccgagg ccgacgaagg cccccgtggc
    12721 cgagaggagg aggctctcgg cgaggatgag cgtcaggatg agcgacttgc cgaagccgag
    12781 cgtccggagg acggcgatct ccgtcacccg ctcgcgggcg gccatcgcca tcgtgttcgc
    12841 cgagatgagg aggatgacga gggcgatcgc ggtcgagatc ccggtgacga ccgccttcac
    12901 gttcccgagc atcgagacga agccgttctg gaactccttc tccgtctccg tcttcgtcgg
    12961 gaaggaggag ttctcgaaga gggcgtcgat ctgccgaggg aggctctgga cggcttccgc
    13021 cgacgcggcc ttgatccaga acgtcccgac ctgccccttc gcccagggga ccgcctcctc
    13081 gaccgccttc cggtcgaagt agacgcccgt ctcgtcggag ccgcggaaga cggcgcggac
    13141 ggtcagctcg aggtcgatcg ggaagatgtc gcccttcagg acgaacgagt cgccgatctt
    13201 ccacccgtag cgcttcatga gccgctcgcc gacgagggcg cctttccggt cggaggccca
    13261 ctcggccgcc gttcccgcga cgatcgtcac ctcgtcgaag atcttcacga gcttctccgg
    13321 ctcgcagccg aagcgggcga acatgttctc gggccggttg tccttgtacg tccccccgaa
    13381 ccagttgagg atcgtcacct cctcgacacc ggggagctgc gcgatccgct cgcggtaggc
    13441 ctcggggatg aagttcgcga gggagacctt gtgtcgcacg acgaggcgga acatgccctt
    13501 gccgcccgac gggtcggcgt cgagcgtcgc gaggagcgtg ccgaggacgc agatgacgaa
    13561 gaaggggagg acgatcgaga ggatcgtcag gaccgagcgc gtcctcttgc ggaagaggct
    13621 cttcaggacg tacgggacga acttcatgcg acctgccgga gcccctccgt gatcggccgc
    13681 tgcgcggaac ggatcgccgg gacgaggccg gcgaagaggc cgacggcgaa ggtcatcccg
    13741 aaaccgagga cgaggacctc ggggaagagg cggatccccg cggcgaacga cgccatgggt
    13801 gagttgacga tcccttctcg gagtccggga aggaggagga cgaacaggcc gatgccgagc
    13861 ccgcccccga tcatcgagag cgcgaagctc tcgccgagga cgaggccgag gaccttctcc
    13921 ttcgtgaagc cgaggacgcg gaggaccgcg atctcggtga cccgctcgcg agcggccatc
    13981 gccatcgtgt tcgcggcgat cagcaggatg acgagggcga tcgccgtcga gatccccacg
    14041 acgacggcct tgacgttccc gatcatcgag acccacatgg cctggaactg cttctcggtt
    14101 tccgtccgcg tcggctccgg cgagttctcg aacatcgcgt cgatgagcct cgggacgcgc
    14161 tccgtatcgg cgacggacgc cgtcttcagc cagtaccagc cgacctggcc cttcgcgtag
    14221 gggacggcct cctcgatacc cttgtgatgg aagtagaccc cgctctcgtc gggtgcctcg
    14281 aagacggcgc ggatcgtcag ctcgagcgtc accgggtaga tgtcgccctt gaaggtgacc
    14341 ttctgcccga gcctccagcc gtacttcctc atgagctggc ggccgacgag gagaccggtg
    14401 cgatcgccga cccagtcggc ctcgctccct tcgacgatgg tcgcctcgtc gtagacacga
    14461 agaaggcggt cgacgtcggt gacggagaag cgctggaact ggttcttcgc cgactggtcg
    14521 atgtaggccc ctccgaacca ctgcatccgt acggcctcgg tcacgcccgg gacgttccgg
    14581 atcttcgggt cgtaggcacc gggaatccag ttcgtgatcg agaccttgtg ccggacgatg
    14641 agccggtaca tccccttccc gcccgaggga tcggtctcga ggcccctcag gagcgtcccg
    14701 aggatgcaga tgacgaagag cggcaggacg atcgaggcga tcgtcagcgc cgagcgcgtc
    14761 ttcttcctca ggaggttctt caggacgagc gggaggatct tcatcgccct acttgccggc
    14821 ggcgatcgcc gcggcggcgg cgaccccgat ctcccccttc tcgcccttcg cgcggacgtc
    14881 ctccttgagg acccccttgt cgaggtggac gaggcggtga gcgcgggccg cggccttcgg
    14941 gtcgtgcgtc accatgacga tcgtcttgcc gaactcggcg tggagccgct cgaggaggac
    15001 gaggatctcc tcgcccgact tcgcgtcgag gtcgcccgtc ggctcgtcgg cgacgaggag
//
LOCUS       DAOONL010000083        15014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4231_length_15014_cov_18.379571, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000083 DAOONL010000000
VERSION     DAOONL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4231_length_15014_cov_18.379571"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PLP50_12075"
     CDS             <1..158
                     /locus_tag="PLP50_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52328.1"
                     /translation="YWDRPEQLGGAGEVVPVDLFVPGCPPHPLTILDGLLRLLGRLED
                     EGRVPSR"
     gene            236..595
                     /locus_tag="PLP50_12080"
     CDS             236..595
                     /locus_tag="PLP50_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPA52329.1"
                     /translation="MHELSLVTEIYRTARRAVEGSGGGRLEVVRLAVGELAVVEPDLL
                     GFAWEAVSAGTPDAGSRLDVDFRPARQTYAACGDVPERAAGSWLRLCPRCDGPLGIEG
                     GDELDVLNVTFEQDAKS"
     gene            720..1613
                     /locus_tag="PLP50_12085"
     CDS             720..1613
                     /locus_tag="PLP50_12085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA52330.1"
                     /translation="MTLDGDGRPLPAAPENLPREIGGFQILGKLGEGGMGIVYEAEQR
                     SPRRRVALKVVRGGQFVDETYLRMFRREAETLARLVHPNIAAIYEAGRTDDGQHFFTM
                     ELVAGRTLSVYARERLGGERPGIEALRERMRLFATVCRAVSHAHQRGVIHRDLKPSNI
                     VVSEAGEVKVLDFGLARITDTDVAVGTVRTELGKIWGTLPYMSPEQTRGDSRDIDFRS
                     DVYSLGVVLYELLTGRKPYDTPTGSVVQAIRAVCEAPPRPLREVSGGAVVDADLQTIV
                     AKALEKEPDQRYQSTVGSSIL"
     gene            complement(1840..3099)
                     /locus_tag="PLP50_12090"
     CDS             complement(1840..3099)
                     /locus_tag="PLP50_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA52331.1"
                     /translation="MLLDCLHETVKGAWEDLFERRYGFWRGLLDSVVARYLDCGIFQR
                     GFARIRCPDCAAEYLLAFSCKGRGLCPSCGAKRAAEFAAFLKDEVVADVGHAQWVFTV
                     PKLVRPYFMHHRELLWPLCTAAWKTVRELMAVAADEEQGFRPGMVAMSQTFGDQLNLH
                     PHVHALVTRGGWTVSGQWVPVPHVSPSAAETLFRHKVIRLLQREGLLDEDRTRLLLSW
                     HHSGSSAHNSATVPAGDGRALEALVRYCLQSPVSLARLRWDPGSQTATYLPRDGHDEE
                     KAETLDALEFVARLLAHVPDPRRHLVHYYGACFNVVRGKVKARGQAQQGASLAPGPGV
                     HPPPPPASVTPSLAALRRGWAQLIRRVYEVDPLVCPRCQGVMCVVAFITEPRVIRRIL
                     DHLGAPARRAVQDRAPPLVAAPVTDSP"
     gene            complement(3422..3615)
                     /locus_tag="PLP50_12095"
                     /pseudo
     CDS             complement(3422..3615)
                     /locus_tag="PLP50_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633058.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3693..4139)
                     /locus_tag="PLP50_12100"
     CDS             complement(3693..4139)
                     /locus_tag="PLP50_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA52332.1"
                     /translation="MLLFDINVLVHAHRRDAPDHTKYRKWLEATLGADSAYGVSDLVL
                     SGFLRVVTHPKVFREPTPLESALAFAHEVRGRPNAVGIAPGPRHWEIFTRLCQECGVR
                     GNLVPDAWLAALAVESGCEWVTTDRDFARFPGLRWRHPLQSTPRPR"
     gene            complement(4120..4377)
                     /locus_tag="PLP50_12105"
     CDS             complement(4120..4377)
                     /locus_tag="PLP50_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HPA52333.1"
                     /translation="MRTTIHLPDPLLDQARRRALETGTTLTGVIEDALRESLAGKRTG
                     KKASPVRLTTFGGKGPRPGVDLDDGAALLDVMEEPRAPVRH"
     gene            complement(4422..7985)
                     /gene="dnaE"
                     /locus_tag="PLP50_12110"
     CDS             complement(4422..7985)
                     /gene="dnaE"
                     /locus_tag="PLP50_12110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPA52334.1"
                     /translation="MGEIGPPARDMFMPGDARKIVSPGARKEELRKRRFGHPSAASRV
                     RWAELRCASAFSFLEGASEPETLVERAAALGLPAVALVDRNGVSGAPRFFKAARAAGL
                     KAIVGAEVVLDEAALPKDLRGPLGLVPPPPPPGLPPARVTLLAESRTGYRNLSRLLTA
                     AALGKPKGQAAASWSDVAAHAEGLHVLSGGIEGPVLRALERVGAEAAGKLLQRLAAIF
                     PGRLHVELQRHHVRDEEARNAVLLDLARAQKLPLVATNGTRYARRRDKDLFDVLTCIR
                     NHTTVDSAGRLLEAGSERHLKGPEEMAALFADLPGAVGGAADLADRLAFTLADLGYRF
                     PDAKVPPGETPSSWLRQKTWEGARSRFQPLTAKAQDQLKRELDLIEKLDLAGYFLVVH
                     DVVEFCRRENILVQGRGSAANSAVCYALSITAVDPVKMELLFERFLSEERGEWPDIDL
                     DLPSGDAREKVIQHVYATYGVHGAAMTANCITYRDRSAARETAKALGFSTEQVDVLAK
                     RLATWSFGEYRDAAGSLTARETGDGDPKEAAAARAAAFAEDGPYSNTTGPPPLPEKMA
                     EEMRAAGFDPDDVRLRHFGSLFRRIQNLPRHLGQHSGGMVVAGGRLDEVVPLEPAAMP
                     GRVVIQWDKDDCADLGIIKIDLLGLGMLGAIAEMVPTIHAAEGVHVDLAHLPHDDPAV
                     YEILNRADTVGLFQVESRAQMASLPRNAPRCFYDLVVQVAIIRPGPIQGGMVHPFFDR
                     RQGRIPVEYPHPSLEPILKRTLGVPLFQEQLLKIAMVAAGFSGGEAEELRRAMGFKRS
                     SERMAVIEGRLRAGMAERGIVGEAQEQIVRSITSFALYGFPESHAASFALIAWASGWL
                     KVHHPAAFLAGLLNAWPMGFYHPATLVKDAQHHGTDVLPIDVTRSGRLCRVERLRGPW
                     PKHLPRPSPAVVTPSLAVRLGMRYVRGLRASTADAIEREAARAPFSGVKDLARRAALR
                     EDELSALAHAGAFATLPDAATGETGLSRRTALWQAASAARDLGPLYDALEDDVASPLP
                     EMSRFEETVSDYQTTEMTAGPHLIAHFRPQLKKDGILSAADLAKAPDGSTVKTAGVIV
                     VRQRPGTAKGFVFLSLEDETGMCQAILRPDLFRENRSLVVGSPALVVEGRLQKKDGTL
                     SIRAERFERMSLDEPIPERRKPVPPPYPRHRESTPPSHDFR"
     gene            8233..10350
                     /gene="tssI"
                     /locus_tag="PLP50_12115"
     CDS             8233..10350
                     /gene="tssI"
                     /locus_tag="PLP50_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HPA52335.1"
                     /translation="MGEYLQQQRKLRVTTPLGENELLLKGFKGEEGISRLFSYRLEMI
                     AENKTKVAFDAVLGQKVTVHLQLPDESETHLNGLCVRLAQGERDEKFTLYEAELVPDV
                     WRLTRKAQSRIFQRMTVPEILKKVFTGFDADWQLTGKYEPRDYCAQYRETDFSFGSRL
                     MEEEGIYYFFEHSDGSHKMIVSDNPLEHPTVAGESRITYEELEGGTREDNRIWGWKKE
                     QEIRPGKYVLWDHSFELPHKHLEAETLTLDSVPVGKETHKLKVAGVDAMELYDYPGEY
                     AQRFDGIGPAGGEQPAELQKIFRDNQRTVELRMDEETSTAVVLRGRSNVKHLLSGYKF
                     TLQRHFSGDGDYVVTSLRHEATYGADYRSGQDVTMTYENTFTCIPAALPFRPERKTAK
                     PVVQGSQTAVVVGPSGEEIFTDKYGRVKVQFHWDREGKNDENSSCWVRVATSWAGRNW
                     GAIRIPRIGQEVVVGFLEGDPDRPIVVGSVYNADQMPPWDLPANKTRSGVKSRSSKGG
                     GPANFNEIMFEDKKGGELLKIHAERNQSISVEADETHTVGNDRTKSVSNNETTSIGND
                     RTETVTNNEMITVGVSRTIMVGGAETRTIGGAETIAVGGLYKLAIGGACSTTVGGGST
                     TNVGADASTQVGGSAATTASKSVSITAGNAVQIQSGKAGITLKKDGTIVIKGKNITLD
                     ASGKINIKAASSVTIKGSKIQEN"
     gene            10385..11386
                     /locus_tag="PLP50_12120"
     CDS             10385..11386
                     /locus_tag="PLP50_12120"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HPA52336.1"
                     /translation="MDLVNRSPFAAERALVLDESGAETLAVLLKASFVWAPRTGLAPA
                     PEQVPVRLADEHLGEPGRSSVLRASDLCPGKPGCDLVLLGSAGRSRPAPQVDVSFRVG
                     AWGRELRVFGERRWSGSAGVPDASPFESIDLTWENAFGGRDEDVPPGEPWEEELRNPV
                     GRGFRARKSRRPVSGDLLPSIEDPAEPIRGPLDRPAPAGVGFVAPHWEPRRSRAGTYD
                     EAWQRGRAPLPPEDFDPRSRQRGASGLVAPEAPAAGAPVVVTGVRPHGEVRFALPRVV
                     PSGAIYFRTKKSDLSFALQTVLFDANASLLELTFGAYQSVHGQTRDVECVAVEAEAS"
     gene            11383..12501
                     /locus_tag="PLP50_12125"
     CDS             11383..12501
                     /locus_tag="PLP50_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPA52337.1"
                     /translation="MSAGAPRSGARGGIAITGLGAVTAVGATLDQTATSLQAGVQRMV
                     ERPLAGEGGEPPEEGGVYLAASAEALAASLGSADPVALALAALREAFWDAGLYEPGDV
                     DAAYRRIALSVHLALPYAEAPGAVAVDTFVEGAVAAGLLAEEPAVEVVPFRNGHAGAL
                     LALESAAARLQGGEIDVALVGGLDATLDPARIAELNRLSKLRTDRAAGGAVPGEGTAF
                     LVVERLADARRRGARFAAAVESVSRDTETVPLDGTRPGRADGATRAVTRALGAAPSAR
                     RVEEIWCDLNGERSRALEWGFLETRALSALGGARRQTPAAFAGDLGSSSGALLLALAA
                     HALRTPRGAGGAALVCGASERGERAAAVLAIPRAGGEG"
     gene            12507..13973
                     /locus_tag="PLP50_12130"
     CDS             12507..13973
                     /locus_tag="PLP50_12130"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HPA52338.1"
                     /translation="MPTTTVNMLTVVHKKSGGISTAFPDICKTPTPAGGTPVPVPYPN
                     IAFSKDTANGSTTVKVDGQSIMLLKSNFSTSTGDEPGSLGGIISQIVKGKAQPLTASF
                     DVTVDGDFVFRLFDLMLQNANPPPNTPPGVLIQAPLTPYIPWLNPPKWQVRWSKGEAK
                     CGDEVDILVQAMGGDPGYYLSMTVARAPHDLPVTYFVTAISGGSAKIPWVTLTGPSPK
                     NKVQLDIYAFGAGEYAKSSSPLKIVVPEEVREAGSKRRRTVPKVTPVLQPDGTYTYTQ
                     GTGRWGWDYGYDLEIKDGILKVTGRIKLVARGGAVFGARHKRGWKAEIEAIWTRKWRE
                     HRKKCKRGKDCKCWGGCCLFPVVFECLFVSSGEFAEVNVWAGNPDTYGTDASGAHIKW
                     WNSGNWFMERCGHEGNQAGVRAHEFGHTLGLYDEYRRGAIFVPTDASGNVTGPPPRAN
                     VPGSLMGPNGLGIKRNHLDEFHQWFLGKANDEYTRERL"
     gene            14141..14986
                     /locus_tag="PLP50_12135"
     CDS             14141..14986
                     /locus_tag="PLP50_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52339.1"
                     /translation="MAESWVFFTRSVGSAGTLGASFTADAALVRTDASLPGIPRIGFF
                     RRPAEAGEFSRLATSIRALGAPRESGTHFPGAPLVSLGIMTNGKAEVVHEQEESELNP
                     AEKQVSSSLEATVEKVLEHPHKALEASIEWVRGTIEPDDELAILVRVSNPGLAPVPFV
                     HPAGREEGESGLAVVLARVGPDGVAVEAREVTFEASEVSEIGADGKPIAGPYTATVEL
                     APGKDLRLRARSRVRFVPASYRASARIDVEAPPDSDEKAVAGTISVELPALGVVRPGA
                     KRGKD"
BASE COUNT         2265 a         5029 c         5715 g         2005 t
ORIGIN      
        1 cgtactggga ccggcccgaa cagctcggcg gcgcgggcga ggtcgtcccg gtggacctct
       61 tcgtcccggg ctgcccgccc cacccgctca cgatcctcga cggtctcctt cgcctcctcg
      121 ggaggctcga ggacgagggg cgcgtccctt cccgctgaga tgaccgtgcc cgccgcgcta
      181 cccgagccgg gaccgtcacg ttccgcctct ccgtcctgac gcaaggagac ccgcgatgca
      241 cgagctctcc ctcgtcaccg agatctaccg caccgcccgt cgcgccgtcg agggcagcgg
      301 cggcggccgc ctcgaggtcg tccgcctggc cgtcggcgag ctcgcggtcg tcgagcccga
      361 cctcctcggc ttcgcgtggg aggcggtgtc ggcgggcacg cccgacgccg gctcccgcct
      421 cgacgtcgac ttccgccccg cgcgccagac ctacgccgcc tgcggcgacg tccccgagcg
      481 cgccgcgggc agctggcttc gcctctgccc gcgctgcgac gggccgctcg ggatcgaggg
      541 gggcgacgag ctggacgtcc tgaacgtgac gttcgagcag gacgcgaagt cctgacgggc
      601 aggggtgaag aggacgacgg gggcggcgct gcgccggttc gctccgaaac gcctgccggg
      661 ctaggctcgt ctccatcgtc accgagagag acgagaacga aggttcgtac agtgatgccc
      721 tgaccctcga cggcgacggc cggccccttc ccgccgcacc ggagaacctc ccccgcgaga
      781 tcgggggctt tcagatcctc gggaagctcg gcgagggagg gatggggatc gtctacgagg
      841 ccgagcagcg gagcccgcgc cggcgggtcg cactgaaggt cgtccgcggc ggccagttcg
      901 tcgacgagac ctacctccgg atgttccgcc gggaggcgga gacgctcgcg cgcctcgtcc
      961 acccgaacat cgcggcgatc tacgaggcgg gccggaccga cgacgggcag cacttcttca
     1021 ctatggagct cgtcgcgggc cggacgctga gcgtctacgc gcgggaacgc ctcggcggcg
     1081 agcggcccgg gatcgaggcg ctccgggagc ggatgcggct ctttgcgacc gtctgccggg
     1141 ccgtgagcca cgcccaccag cgcggggtca tccaccggga cctgaagccc tcgaacatcg
     1201 tcgtgtccga ggcgggcgag gtgaaggtcc tcgacttcgg cctcgcgcgg atcaccgaca
     1261 cggacgtcgc cgtcgggacg gtgaggaccg agctcgggaa gatctggggg acgctgccgt
     1321 acatgagccc ggagcagacg cggggcgaca gccgcgacat cgacttccgg tcggacgtct
     1381 actcgctcgg cgtcgtcctc tacgagctgc tgacgggccg gaagccctac gacacgccga
     1441 cgggctccgt cgtgcaggcc atccgggcgg tctgcgaggc cccgccccgg cccctgcggg
     1501 aggtctccgg cggcgcggtc gtcgacgccg acctccagac gatcgtcgcg aaggcgctcg
     1561 agaaggagcc ggaccagcga taccagtcaa cggtaggaag ctcgattctc tagggcgatg
     1621 cggctgaagg ccggggcggg ctcaaggggt ctaagacgcc ctgagggagc ggcgccgcgg
     1681 cggcgccgct ccctcggggc gagatgtcac ggcaggtcgg cgcagtctct gagcggtttc
     1741 tcgggagcac gagggggggg gccggtcgtc gggctgtggt cgaagcggcc ccgggcctgc
     1801 cgcgcgagtg gaggcatcgg ccccggccgt cccgggggat cagggggaat cggtgaccgg
     1861 ggccgcgacg agagggggag cgcgatcctg gacagcccgc cgagccgggg cgccaaggtg
     1921 gtcgaggatg cggcggatga cgcggggctc ggtgatgaag gcgaccacgc acatgacgcc
     1981 ctggcagcga ggacagacga gcggatcgac ttcgtacacg cgacgaatga gctgggccca
     2041 tccacggcga agggcggcca aagagggggt caccgaggcc ggcggaggcg gagggtgaac
     2101 gcccggcccc ggcgcgagcg acgcaccttg ctgagcctgg ccgcgagcct tcaccttccc
     2161 gcgtacgacg ttgaagcagg cgccgtagta atggacgagg tgccttctcg gatcgggaac
     2221 gtgcgcgagg agacgggcga cgaactcgag ggcgtcgagg gtctcggcct tttcctcgtc
     2281 gtggccgtcc cggggcagat aggtggcggt ctgagacccc ggatcccagc gcagacgggc
     2341 gaggctcacg gggctctgca ggcagtagcg gacgagagcc tcgagggcgc ggccgtcgcc
     2401 ggctggaacg gtcgcggagt tgtgggccga agatcctgag tgatgccagg agaggagcag
     2461 gcgggtgcgg tcctcgtcga ggaggccttc gcgctgcagg agacggatca ccttgtgccg
     2521 gaagagcgtc tcggcggcgg aggggctgac gtgcggcacg ggtacccact ggccggagac
     2581 ggtccagccg ccacgggtga cgagcgcgtg gacgtgagga tgtaggttga gctggtcccc
     2641 gaacgtctgc gacatcgcga ccataccggg gcgaaagccc tgctcttcgt cggccgccac
     2701 ggccatcagc tcgcggacgg tcttccaggc cgcggtgcag aggggccaca gcagctctcg
     2761 gtgatgcatg aagtaggggc gaacgagctt ggggacggtg aagacccact gggcgtggcc
     2821 gacgtcggcg acgacctcgt ccttcaggaa ggcggcgaac tcggcggcgc gcttggcgcc
     2881 gcaggatggg cagagcccgc gccctttgca cgagaaggcg agcaggtatt cggcggcgca
     2941 atcgggacag cggatgcggg cgaagcctcg ctggaagatg ccgcagtcga ggtaccgcgc
     3001 gaccacgctg tcgaggaggc cgcgccagaa gccataccgg cgctcgaaga ggtcttccca
     3061 ggcgcccttg accgtctcgt ggaggcaatc gagaagcacg aacaggggcg tctcggcggg
     3121 cctgcgcgcc cgatacgagg ctgcttccac gggacgaggc catggcgggc ctcgtgccgg
     3181 agggccggag acagtgcaag tgcgcgtggg acagaggctt cagcgcgggt gggcgatgca
     3241 ggtcggggcc ggggcgacag gagaacctga cgaaggcggt gggctacgag tgcgtactcg
     3301 ctgccgtacc aggggatcgg taacggcaac gaaaccggtc cgcgctgaca aagctgcctg
     3361 tcggcgaccc cgtgaggctc ctccacgccg gcaccgcggc cccgttcccg ggtgagcccg
     3421 tctacgcgtg gacgcccggc gcctcgtggc ccgaccgcgc gacgtactcc ccacaggtcc
     3481 cgccgtaggt gacgggcttg ccggtccgtc ctcgcctccg atctccagaa cccgcttcgc
     3541 caggccccgg aggaacgtcc ggtcgtgcga gacgaagagc atcgtccccc cgaacgcgtg
     3601 gagcgcttcg acgagccatc gcggggccgg cggcttccgt cgtcaccgga agtcgtggct
     3661 cggcggcgtt gcgacgaggt gcgggccggc ggtcatctcg gccgaggggt gctctgcagc
     3721 gggtgccgcc acctgagccc cgggaaacgc gcgaagtcgc ggtcggtcgt cacccactcg
     3781 caccccgact ccaccgccag cgccgcgagc caggcgtctg gcacgagatt cccgcgcacg
     3841 ccgcactcct ggcagagccg ggtgaagatc tcccagtgtc tcgggcccgg cgcgatcccg
     3901 acggcgttcg gccggccccg gacctcgtgg gcgaaggcga gcgcgctctc gagcggcgtc
     3961 ggctcgcgaa agaccttcgg gtgtgtgacg acgcgcagga acccgctcag gacgaggtcg
     4021 gagacgccgt aggcggagtc ggccccgagg gtcgcctcga gccacttccg gtacttcgtg
     4081 tggtcggggg cgtcgcggcg gtgggcgtgg acgagaacgt taatgtcgaa caggagcacg
     4141 cggctcctcc atgacgtcga ggagcgcggc accgtcgtcc aggtcgacgc cgggccgggg
     4201 cccctttccg ccgaaagtcg tcagccgcac cggtgaggcc ttcttcccgg tgcgcttgcc
     4261 cgcgagggac tcccggagcg cgtcctcgat caccccggtc agggtcgttc ccgtctcgag
     4321 ggcacggcgg cgagcctgat ccagcagggg atcggggagg tggatcgtcg tcctcacaca
     4381 tcaaagcata tcaaaggcgc atcgtgatgc atcactcgcc ttcatcggaa atcgtggctc
     4441 gggggcgtgc tctcgcggtg gcgtgggtag ggcggtggga cgggcttcct ccgctcgggg
     4501 atcggctcgt cgaggctcat cctctcgaag cgctcggcgc ggatcgaaag ggtgccgtcc
     4561 ttcttctgga ggcgcccctc gacgacgagg gcgggggagc cgacgacgag ggagcggttc
     4621 tccctgaaga ggtcggggcg gaggatcgcc tggcacatcc ccgtctcgtc ctcgagcgag
     4681 aggaagacga agcccttcgc ggtgccgggc cgctgccgca cgacgatgac gccggccgtc
     4741 ttcaccgtcg acccgtcggg ggccttcgcg aggtccgcgg cggagaggat tccgtccttc
     4801 ttcagctgcg ggcggaagtg ggcgatcagg tgcgggccgg cggtcatctc ggtcgtctgg
     4861 tagtcggaga cggtctcctc gaaacggctc atctcgggga ggggcgaggc gacgtcgtcc
     4921 tcgagggcgt cgtagagcgg gcccaggtcg cgcgcggccg aagccgcctg ccagagggcg
     4981 gtccggcgcg agaggcccgt ctcgcccgtg gccgcgtcgg ggagcgtcgc gaaggcgccg
     5041 gcgtgggcga gggcggagag ctcgtcctcg cggagggcgg cgcggcgggc gaggtccttg
     5101 acgcccgaga acggcgcgcg cgcggcctcg cgctcgatgg cgtcggccgt cgaggcgcga
     5161 aggcccctca cgtagcgcat cccgaggcgg acggcgagag acggcgtgac gacggcgggc
     5221 gagggccgcg gcaggtgctt cggccacggg ccgcggaggc gctcgacgcg gcagaggcgg
     5281 ccggagcggg tgacgtcgat ggggaggacg tccgtcccgt ggtgctgcgc gtccttcacg
     5341 agcgtcgccg ggtggtagaa gcccatcggc caggcgttga ggaggccggc gaggaaggcg
     5401 gccggatggt ggaccttcag ccagccgctc gcccaggcga tgagggcgaa gctcgccgcg
     5461 tgcgactcgg ggaagccgta gagcgcgaac gacgtgatcg accggacgat ctgctcctgc
     5521 gcctcgccga cgatgccgcg ctcggccatc ccggcgcgga ggcgcccctc gatgaccgcc
     5581 atccgctcgc tcgagcgctt gaagcccatc gcgcggcgga gctcctccgc ctcgccgccg
     5641 ctgaagccgg ccgcgaccat ggcgatcttc aggagctgct cctggaagag cgggacgccg
     5701 agggtgcgct tgaggatcgg ctcgagcgac gggtgcgggt actcgaccgg gatgcgcccc
     5761 tgccggcggt cgaagaacgg gtggaccatg cccccctgga tggggccggg ccggatgatc
     5821 gcgacctgga cgacgaggtc gtagaagcag cgcggggcgt tgcgggggag cgaggccatc
     5881 tgcgcgcggc tctccacctg gaagagcccg accgtgtcgg ccctgttcag gatctcgtag
     5941 acggccgggt cgtcgtgcgg gaggtgcgcg aggtcgacgt ggacgccttc ggccgcgtgg
     6001 atcgtcggga ccatctcggc gatggccccg agcatcccga ggccgaggag gtcgatcttg
     6061 atgatgccga ggtcggcgca gtcgtccttg tcccactgga tgacgacgcg ccccggcatc
     6121 gcggcgggct cgagcgggac gacctcgtcg agccgccctc ccgcgacgac catcccgccc
     6181 gagtgctggc cgaggtggcg cgggaggttc tggatgcggc ggaagaggga gccgaagtgc
     6241 cggagcctca catcgtcggg gtcgaagccc gcggcgcgca tctcctcggc catcttctcg
     6301 gggaggggcg gcggacccgt cgtgtttgaa tagggcccgt cctcggcgaa ggccgcggcg
     6361 cgcgccgcgg ctgcctcctt cggatcgccg tcgcccgtct cgcgcgccgt gagcgagccg
     6421 gcggcatccc ggtattcccc gaagctccac gtcgcgagcc tcttcgcgag cacgtcgacc
     6481 tgctccgtcg agaagccgag cgccttggcc gtctcgcgcg cggcggagcg gtcgcggtag
     6541 gtgatgcagt tcgcggtcat cgccgcgccg tggacgccgt acgtcgcgta gacgtgctgg
     6601 atcaccttct cgcgggcgtc gcccgagggg aggtcgaggt cgatgtcggg ccactcgccc
     6661 cgctcctccg agaggaagcg ctcgaaaagg agctccatct tcaccgggtc gaccgcggtg
     6721 atcgagaggg cgtagcagac ggcgctgttc gcggccgagc cgcgcccctg gacgaggatg
     6781 ttctcgcgcc ggcagaactc gacgacgtcg tggacgacga ggaagtagcc cgcgaggtcg
     6841 agcttctcga tcaggtcgag ctcgcgcttg agctggtcct gcgccttcgc cgtcagcggc
     6901 tggaagcgcg accgcgcccc ctcccaggtc ttctgcctca gccacgagga gggcgtctcg
     6961 cccggcggga ccttcgcgtc ggggaagcgg tagccgaggt cggcgagcgt gaacgcgagg
     7021 cggtcggcga ggtcggccgc gccgccgacg gcgccgggga ggtcggcgaa gagggcggcc
     7081 atctcctcgg gccccttgag gtgccgctct gagcccgcct cgaggagacg cccggcggaa
     7141 tcgaccgtcg tgtggttccg gatgcaggtg aggacgtcga agaggtcctt gtcgcgacgc
     7201 cgcgcatagc gggtgccgtt cgtggcgacg agggggagct tctgcgcgcg ggcgaggtcg
     7261 aggaggacgg cgttccgcgc ctcctcgtcg cggacgtggt gccgctgcag ctcgacgtgg
     7321 agccgcccgg ggaagatcgc cgcgaggcgc tgaaggagct tgcccgcggc ctcggccccg
     7381 acgcgctcga gagcgcgcag gacgggcccc tcgatccctc ccgagaggac gtggagcccc
     7441 tcggcgtgcg cggccacgtc ggaccacgag gctgcggcct gccccttcgg cttgccgagc
     7501 gcggcggcgg tcaggaggcg gctgaggttg cgatagcccg ttcggctctc ggcgaggagc
     7561 gtgacgcgcg cgggcggcag gcccggcggg ggaggaggcg ggacgaggcc gagcggcccg
     7621 cggaggtcct tcgggagggc cgcctcgtcg aggacgacct cggccccgac gatcgccttc
     7681 aggcccgcgg cccgtgcggc cttgaagaaa cggggcgcgc cggagacgcc gttgcggtcg
     7741 acgagggcga cggcggggag gccgagcgcg gcggcccgct cgacgagcgt ctcgggctcc
     7801 gaggccccct cgaggaatga gaaggccgag gcgcaacgca gctcggccca ccggaccctt
     7861 gacgcggcgg agggatgccc gaagcggcgc ttccgcagct cctccttccg cgcgccggga
     7921 gagacgatct tccgcgcgtc gcccggcatg aacatgtcgc gggcgggggg gccgatctcg
     7981 cccattggcg taaattaggc gcaagtgtcg cgagaatcaa gcccgcggct cgaaggagct
     8041 tcgtccgtcg gcgcccgagg ccgtgtcgaa gccgcgcggc gcgcccgtcg cgcgttccgg
     8101 tgagccggct cacggcgatg ccctttgacg acgccgttcc tttgacccga cctgtttcgg
     8161 accctatatt cccgcggcaa gcagaaacgg atcagctctc gaatccgggc cggatgcccg
     8221 ggaggacgga tgatgggcga gtatctccag cagcagcgca agctccgggt gacgacgccg
     8281 ctcggcgaga acgagctcct cctgaagggt ttcaaggggg aggagggaat ctcgaggctc
     8341 ttctcatacc gcctggagat gatcgcggag aacaagacga aggtggcgtt cgacgctgta
     8401 ctggggcaga aggtgaccgt tcacctgcag ctgcccgacg agagcgagac ccacctgaac
     8461 gggctctgcg tgaggctcgc gcagggggag cgggacgaga agttcacgct ctacgaggcg
     8521 gagctggtgc cggacgtctg gaggctgacc cggaaggcgc agagccggat cttccagagg
     8581 atgacggtgc cggagatcct gaagaaggtc ttcaccggct tcgacgcgga ctggcagctg
     8641 accgggaagt acgagccgcg ggactactgc gcgcagtacc gggagacgga cttcagcttc
     8701 gggtcccgcc tgatggagga ggaggggatc tactacttct tcgagcactc cgacgggagc
     8761 cacaagatga tcgtctcgga caacccgctc gagcatccga ccgtcgcggg cgagagccgg
     8821 atcacgtacg aggagctcga aggggggacg cgggaggaca accggatctg gggctggaag
     8881 aaggagcagg agatccggcc cggcaagtac gtcctctggg accacagctt cgagctgccg
     8941 cacaagcacc tcgaggccga gacgctcacg ctcgacagcg tcccggtggg gaaggagacg
     9001 cacaagctga aggtcgcggg cgtcgacgcg atggagctct acgactaccc gggcgagtac
     9061 gcgcagcgct tcgacgggat cggccccgcg ggcggcgagc agcccgcgga gctgcagaag
     9121 atctttcgcg acaaccagcg gacggtcgag ctgcggatgg acgaggagac gtcgacggcg
     9181 gtcgtgctgc gaggccggag caacgtgaag cacctcctct cgggatacaa gttcacgctg
     9241 cagcggcact tcagcgggga cggcgactac gtcgtgacga gcctgcgcca cgaggcgacg
     9301 tacggcgcgg actaccggtc ggggcaggac gtgacgatga cgtacgagaa cacgttcacg
     9361 tgcattcccg cggcgttgcc gttccggccg gagaggaaga cggcgaagcc ggtcgtccag
     9421 ggaagccaga cggcggtggt cgtcgggccc tcgggcgagg agatcttcac ggacaagtac
     9481 ggccgggtga aggtccagtt ccactgggac cgcgagggga agaacgacga gaactcgtcg
     9541 tgctgggtcc gcgtggcgac gagctgggcg gggcggaact ggggggcgat ccggatcccg
     9601 aggatcgggc aggaggtcgt cgtcggcttc ctggagggcg acccggaccg gccgatcgtc
     9661 gtgggcagcg tctacaacgc cgaccagatg ccgccctggg acctgcccgc gaacaagacc
     9721 cgcagcggcg tgaagtcccg gagctcgaag gggggaggcc ccgcgaactt caacgagatc
     9781 atgttcgagg acaagaaggg gggcgagctc ctcaagatcc acgccgagcg gaaccagtcg
     9841 atctcggtcg aggcggacga gacgcacacc gtcggcaacg accggacgaa gtccgtctcg
     9901 aacaacgaga cgacgagcat cggcaacgac cggacggaga ccgtcaccaa caacgagatg
     9961 atcacggtcg gcgtcagccg gacgatcatg gtcggcgggg ccgagacccg gacgatcggc
    10021 ggcgccgaga cgatcgcggt cgggggcctc tacaagctgg ccatcggcgg ggcctgctcc
    10081 acgaccgtcg gcggcggctc gacgacgaac gtcggcgccg acgcgtcgac gcaggtgggt
    10141 gggagcgcgg cgacgacggc gtcgaagtcg gtctcgatca cggcggggaa cgccgtccag
    10201 atccagtcgg gaaaggcggg gatcacgctg aagaaggacg gcacgatcgt catcaagggg
    10261 aagaacatca cgctcgacgc gagcgggaag atcaatatca aggccgcgtc gtccgtgacg
    10321 atcaagggct ccaagatcca ggagaactga gccgagggct cggagcgcga ggagcggcgg
    10381 cacgatggat ctcgtaaacc ggagcccgtt cgcggcggag agggcgctcg tcctcgacga
    10441 gtccggcgcg gagacgctcg ccgtcctcct gaaggcgtcg ttcgtctggg cgccgcggac
    10501 cggcctcgcg ccggcccccg agcaggtccc cgtccgcctc gccgacgagc atctcggcga
    10561 gcccgggcgc tcctcggtcc tccgcgcctc ggacctctgc cccggcaagc cggggtgcga
    10621 cctcgtcctc ctcggcagcg ccggacgctc gcgccccgcg ccgcaggtcg acgtttcgtt
    10681 ccgggtcggt gcgtggggcc gggagctccg cgtcttcggc gagcggcgct ggagcgggtc
    10741 tgccggagtc cccgacgcct cgcccttcga gtcgatcgac ctgacgtggg agaacgcgtt
    10801 cggcgggcgc gacgaggacg tccctcccgg cgagccctgg gaggaggagc tgcggaatcc
    10861 cgtggggcgc ggcttccgcg cccggaagtc gcggaggccc gtgtcgggcg acctcctgcc
    10921 gagcatcgag gaccccgccg agccgatccg cgggcctctc gaccggcccg cgccggccgg
    10981 ggtcggattc gtggcgccgc actgggagcc gcgacgctct cgggccggga cctacgacga
    11041 ggcgtggcag cgcggccgcg cgccgctccc cccggaggac ttcgacccgc ggagccggca
    11101 gcgcggcgcg tcggggctcg tcgcgccgga ggcccctgcg gccggcgcgc ccgtcgtcgt
    11161 gacgggcgta aggccccacg gcgaggtccg tttcgcgctt ccgcgggtcg tcccctcggg
    11221 cgcgatctac ttccggacga agaagagcga tctttcgttc gcgctccaga ccgtcctctt
    11281 cgacgcgaac gcctcgctcc tcgagctgac gttcggcgcg taccagagcg tccacgggca
    11341 gacgcgcgac gtcgagtgcg tcgccgtcga ggcggaggcg tcgtgagcgc gggagcgccg
    11401 cgttccgggg cccggggcgg gatcgcgatc acgggcctcg gcgccgtgac ggccgtcggg
    11461 gcgacgctcg accagaccgc gacctcgctg caggccggcg tccagcggat ggtggagcgc
    11521 ccgctcgccg gcgaaggggg ggagcctccg gaagaggggg gcgtctacct cgccgcttcg
    11581 gccgaggcgc tcgcggcgtc gctcggctcg gccgatccgg tcgccctggc cctcgcggcg
    11641 ctccgggagg cgttctggga cgccggactc tacgagccgg gcgacgtgga cgcggcgtac
    11701 cgccggatcg cgctctccgt tcacctcgcc ctgccgtacg ccgaggcgcc gggcgccgtc
    11761 gccgtcgaca cgttcgtcga gggcgcggtc gcggcggggc tcctcgcgga ggagcctgcc
    11821 gtcgaggtcg taccgttccg aaacggacat gccggggcgc tcctcgccct cgagtcggct
    11881 gccgcgcggc tccagggcgg agagatcgac gtcgccctcg tgggcggtct cgacgccacg
    11941 ctcgaccccg cgcggatcgc cgagctgaac cggctctcga agctccggac ggatcgcgcc
    12001 gcgggcgggg cggtcccggg cgaggggacc gcgttcctcg tcgtcgagcg gctcgcggac
    12061 gcgcggcggc gcggcgcccg gttcgcggcc gccgtcgagt cggtgtcgcg ggacaccgag
    12121 acggtcccgc tcgacgggac gcgaccgggg cgcgcggacg gggcgacgcg cgcggtcacg
    12181 cgcgcgctcg gcgcggcgcc ctcggcgcgc cgggtcgagg agatctggtg cgacctcaac
    12241 ggcgagcgca gccgggcgct ggagtggggg ttcctcgaga cgcgggccct ctcggccctc
    12301 ggcggggcgc gccggcagac gccggcggct ttcgcgggcg acctcgggtc ttcttccggc
    12361 gcgctcctgc tggcgctcgc cgcgcacgcc cttcggacgc ctcgcggggc gggcggagcg
    12421 gcgctcgtct gcggggcctc ggagcgtggc gagcgggccg cggcggtcct cgcgattccg
    12481 cgggccggcg gggaagggtg acggcgatgc cgacgacgac ggtcaacatg ctgacggtgg
    12541 tccacaagaa gagcgggggc atctcgaccg ccttccccga tatctgcaag acgccgacgc
    12601 cggcgggcgg gacgcccgtt ccggtccctt acccgaacat cgcgttctcg aaggacaccg
    12661 cgaacgggag caccaccgtg aaggtcgacg ggcagtcgat catgctgctc aagtcgaact
    12721 tctcgacgag cacgggcgac gagcccggct ccctcggcgg gatcatctcg cagatcgtca
    12781 aggggaaagc ccagcccctc accgcctcat tcgacgtcac ggtcgacggc gacttcgtct
    12841 ttcgcctgtt cgacctgatg ctccagaacg cgaacccgcc gccgaacacg ccgccgggag
    12901 tcctgatcca ggctccgctc accccctaca tcccgtggct gaacccgccg aagtggcagg
    12961 tgaggtggag caagggcgag gcgaagtgcg gcgacgaggt cgacatcctc gtccaggcga
    13021 tgggcgggga ccccggctac tacctgagca tgaccgtcgc ccgcgctccg cacgaccttc
    13081 ccgtgaccta cttcgtcacg gcgatctcgg gcgggagcgc gaagatcccc tgggtcacgc
    13141 tgaccggccc gtcaccgaag aacaaggtcc agctcgacat ctacgccttc ggcgcgggcg
    13201 agtacgcgaa gtcgtcgagc cctctgaaga tcgtcgtccc cgaggaggtc cgcgaggcgg
    13261 gctccaagag gcgacggacc gtcccgaaag tgacgcctgt cctccagccg gacggcacct
    13321 acacctacac acaggggacc gggcgctggg gctgggacta cggctacgac ctcgagatca
    13381 aggacggcat cctcaaggtg acggggcgga tcaagctcgt cgcgcgcggc ggcgccgtct
    13441 tcggcgcccg gcacaagcgg ggctggaagg ccgagatcga agcgatctgg acccggaagt
    13501 ggcgcgagca tcggaagaag tgcaagcgcg gcaaggactg caagtgctgg ggggggtgct
    13561 gcctcttccc ggtcgtgttc gagtgcctct tcgtcagctc gggcgagttc gcggaggtga
    13621 acgtctgggc gggcaacccg gatacctacg ggaccgatgc ctcgggcgcc cacatcaagt
    13681 ggtggaactc cggcaactgg ttcatggaga ggtgcggaca cgaagggaac caggcgggcg
    13741 tgagggccca cgagttcgga cacaccctgg gcctctacga cgagtaccgg agaggggcga
    13801 tcttcgtgcc gaccgacgcg agcggcaacg tcacggggcc gcccccgaga gcgaacgtcc
    13861 ccgggagcct catggggccg aacggcctcg ggatcaagcg gaaccacctc gacgagttcc
    13921 accagtggtt cctcggcaag gcgaatgatg agtacacgcg cgagcgcctc tgaccgcgcg
    13981 gcgcggcccg cgcgcggtcc ggccctccgg ccgcgggagg ttcgagatgg atggagcgcc
    14041 gggtctcggc ggattgagag cggcgagcgt cctcttcgcg gcggcgcttg cctgctgcgc
    14101 ggcgccgccg gccccggcgg agggaaaggc ggactcgccg atggctgaga gctgggtctt
    14161 cttcacgcgg agcgtcggct cggccggaac gctcggagcg agcttcaccg cggacgcggc
    14221 gctcgtccgg acggacgcct ccctcccggg catcccccgc atcgggttct ttcgccggcc
    14281 ggcggaggcg ggcgagttca gccgcctcgc gacgtcgatc cgggcgctcg gcgccccgcg
    14341 cgagtccggc acgcacttcc cgggggcgcc cctcgtctcg ctcggaatca tgacgaacgg
    14401 gaaggccgag gtcgttcacg agcaggagga gtcggagctg aacccggccg agaagcaggt
    14461 ctcctcgagc ctcgaggcga cggtcgagaa ggtcctcgag cacccgcaca aggcgctcga
    14521 agcgtcgatc gaatgggtcc gcgggacgat cgaacccgac gacgagctcg cgatcctcgt
    14581 ccgcgtctcc aacccgggcc tcgcgcccgt gccgttcgtc cacccggcgg gccgcgagga
    14641 gggggagtcg ggcctcgccg tcgtcctcgc tcgcgtcggt cccgacggcg tcgcggtcga
    14701 ggcgcgcgag gtgacgttcg aggcgagcga ggtctcggag atcggagccg acgggaagcc
    14761 gatcgccggg ccgtacaccg cgaccgtcga gctcgctccc gggaaggacc tccgcctccg
    14821 ggcccgatcc cgcgtccgct tcgtcccggc gagctaccgc gcgtccgcgc gaatcgacgt
    14881 cgaggcgccg ccggattccg acgagaaggc cgtcgcgggg acgatctcgg tcgagctgcc
    14941 ggccctcggg gtcgtgcggc ccggcgcgaa gagagggaag gactgatgcc cgacacgaag
    15001 aagctcctcg acgt
//
LOCUS       DAOONL010000084        14743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4345_length_14743_cov_14.938045, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000084 DAOONL010000000
VERSION     DAOONL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4345_length_14743_cov_14.938045"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PLP50_12140"
     CDS             <1..1170
                     /locus_tag="PLP50_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA52340.1"
                     /translation="RIDDPITFDLDPIRKVILSLHPAVPAALVPPFTLDVQDDLYERA
                     DVRLTWPVFAGGRIDAARDAARAREADAGAQLRAVGDALATEVVRRYTAVRLAERARA
                     IRAEVLSALDEHLRNARRLEEEGFLSRAERLHAEVARTEAERELKASGHDLALAREAL
                     ANVLSLPGEEADGLVTTTPLFVPTELPPLPELKAAARDANPALARLEAAASLASAGLA
                     AEKGRWYPEVALFGVRELYKPGLTLLDPTWAAGLSARITLFDGFAREKGIAAARERGA
                     RVAELATRARRDVETLVEKRYREARKAQEQVEAFGAALELARENVRVRTRAFEEGVAT
                     SLDVVDARLSLAKVELGRLAAARDLDVALAELLEAAGRTESYEALRADGAGDVEK"
     assembly_gap    1220..1319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1322..2308
                     /locus_tag="PLP50_12145"
     CDS             1322..2308
                     /locus_tag="PLP50_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52341.1"
                     /translation="MNERTKGLLALGGGVAVLLLLGLAALLLSRPGPETLQGEADATK
                     VDVAAKVAGRLATIDVKEGDTVARGALIATIESPEIRAKLDQAEAAKAGAVAQRDKAD
                     RGAREEEIRAAKSQWDRAKHAAELAETTFGRLERLARDGVVPRQRRDEAEAQWKTSRD
                     AAEAAKAVYDMAVAGARREDRAAAAALVTRAEGAVDEVKAFLDETKVVSPAAGEVYRR
                     NAEPGEIVPAGFPIVTLVDLSDVWVTFQVREDRLARLKMGTSVPVRFPALGGEEVPLT
                     VTFVAPQGDFATWRATSAQGGFDLKTFEVRARPAKPLPGLRPGMSAVVTWPR"
     gene            2296..3480
                     /locus_tag="PLP50_12150"
     CDS             2296..3480
                     /locus_tag="PLP50_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52342.1"
                     /translation="MAPLGSAPGALRAARREAERLVTSRHALVLLVLLPLAGFALATA
                     IFWNRLPEELPVAVVDADRSLLSRRLLRMLDATKSMRVAGPYATGGEALDEVLAGRAY
                     GVVSVPRHFERDVKRGAAPKIVLHYDALTMIPGSILKRDVRAVVGTLSAGVEIQARRA
                     LGETPDVAGARFEPVRSLRGALFNPGLDYRAFLLPAVLATLLTIFVLVAAVHALGVEL
                     RDGTAAEWLASGGSATRAVLGKLLPQSLWFTALGLVAVAVPFRLLEVPLRGSAPLLAL
                     ATLLLVLGYQAVALLLVAFSANLRFASSAAAFYGGPAFAFTGITFPTFGMPLAAQLWG
                     ALLPLTHFLALLLAQAMRGAPASAALGPLGALLAFVLVLPPLSLWRMRRLMSDPAYWG
                     RK"
     assembly_gap    3568..3667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3668..4801
                     /locus_tag="PLP50_12155"
     CDS             <3668..4801
                     /locus_tag="PLP50_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52343.1"
                     /translation="SGALRDPGALLVLVGAIALYAFFYPTPYRREVLKEVPIAVVDLD
                     RTPLSRQLVRFADAHELVRVAAHAGSLAQAEALVRDGKAGGVLGIPEGFEREVKRGAP
                     ASVGVLADATYFLVYRQVLTGLAESVGTLSAGIEIRRLTAQGMPEAKARSFRDPVPLV
                     SRPLFNPAEGYATYVVPPVLILILQQTLLIGIGLRGPADLGRLGSGPLVARAAASVTG
                     RTLAFVALYLVHALFYFGVVARLFDFPVRGSAAAAAGLVLPFLLATSFLGLALSVLFR
                     RREAAIQTLLFTSLPAIFLAGFSWPPAALPAWLRFASQAIPSTSAIPALLRVNQAGAS
                     LAHVRHELALLWGLAVVFFVLAVLAQAGREAAVRRQYRAATKP"
     gene            complement(4774..5541)
                     /locus_tag="PLP50_12160"
     CDS             complement(4774..5541)
                     /locus_tag="PLP50_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52344.1"
                     /translation="MDRFAARIGGGAFTTISRKRVADGLRVRVWAPSAIDQGTSSLCG
                     PASLLFDVATRDPEAYVRYVTALWETGQGRIGRIDVRPGTDLKAFDPGRNVEASDWIA
                     LASLRDSENWFFDYQEDSDALAGITLPGELEGWFRKAGYTEVVNDARAIVDKDEPCLR
                     RADDFFRKGFRVCLFIHSNMLSPSTQANGSATADHWVVQTGRVTFGATMIEGKPATTV
                     SLRIFTWGEGRRAVPQAGVLPLDDFLDNFYGFVAARY"
     gene            complement(5727..6554)
                     /locus_tag="PLP50_12165"
     CDS             complement(5727..6554)
                     /locus_tag="PLP50_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52345.1"
                     /translation="MQDNVDGTTRRADGGFARRRALAAALALLAAFPAAANLRAPRRV
                     PEAPSTALAAPAKELVVERERLTLLCGAEACDVTAEYDVTAGAPERVRLEMVLPVAGD
                     VTARSNGGTGEVSVAPARPFRAEERRSLPEPGPGEPPLHRAAFEAELHEGANTITVRY
                     RQPLGGEEVGHSSRRAGRFVQRFRYELWPLREWTRGPGFRIRLAIGVERPAPGLWQRW
                     FGHPRSVACLTSDPAAPSVEGRLEQRGERLWLDAELGPSFPDRITCYVGDDDLMPRY"
     gene            complement(6621..7805)
                     /locus_tag="PLP50_12170"
     CDS             complement(6621..7805)
                     /locus_tag="PLP50_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPA52346.1"
                     /translation="MSAPQAYERDPYLTELPVRVVATGSEGGRPWAVLDDTVLYPEGG
                     GQPADHGFLGEVPVVDVRTVDGRVRHVLETEVPEGPTVVRLDWARRLDHMQQHTGQHL
                     LTAVAADRFGWETTAFHLGPETCDVELAVPSIVPADAERLEDAVASAIRAALPVTARR
                     VRAEELPGLGVRSRGLPEGHVGDVRLVEIAGVDLNTCGGTHLRSTAELEALKLLGTEK
                     LRGGTRLFFVAGSRVRRRLGEAEERNGRLRALLGAPDPGLAEAVAAKLAQLAEAQKAL
                     RGAEEELAEAKAETLAAAGAPVVDAHFEGRDLAFVGRVARRFTELPVGGLAFLTGEKD
                     GAGVFVLAAGAGSSADVRALGSAVAARLDGRGGGSGKLFQGKASLERRAEALSLLRTP
                     SS"
     gene            complement(7876..8466)
                     /locus_tag="PLP50_12175"
     CDS             complement(7876..8466)
                     /locus_tag="PLP50_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPA52347.1"
                     /translation="MELLDSGRSVLVVIDLQGKLMDVIHRPWLVVEATLRLMKLAELF
                     GVPVVLTEQYPKGLGPTQAEIRAGFDALTVPKRYVDKTSFGCCGEPAFLRALDELRPR
                     ARATRQVLVAGIEAHVCVLQTVVELIHHGNQVHVSWDAVSGRGEEYRRHALERMAAAG
                     AAITNHESVGFEWARDKNHPRFRELSTLLKDGQPSG"
     gene            complement(8539..9663)
                     /locus_tag="PLP50_12180"
     CDS             complement(8539..9663)
                     /locus_tag="PLP50_12180"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52348.1"
                     /translation="MPKKPTPDDLRNLLAPRTEPCISLFLPLPRHGAELAMAPVRFRN
                     LVRQAEALLAERYDAKRIRELIAPLAGLAEAVDVFTPGPEGLAAFRADGVLETYRVWP
                     SPPERAVVADSFHVKPLLRSLQDRNRYHVLAIGTRRVALYDGSERGLVPRALPGLPAP
                     LEPTATGGARNGGGRGARGGPGKAPAEGDGGRREETLRRFRAADAALREALPGATPIL
                     LAGPGNVLPAFREVCTHPAVHPEGVDGAVDDVAVDELHERVLPAARRALEARNARLVE
                     EFLKAQDRGLGSDVLTTVAEAAVAGRVRRLVVAGGKTLFGRVDRETGEVTLHAGQSGP
                     QDDDVLDDLAETVLARGGEVFVLDAAKMPHEVAAVAIYRW"
     gene            9762..11264
                     /locus_tag="PLP50_12185"
     CDS             9762..11264
                     /locus_tag="PLP50_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA52349.1"
                     /translation="MRRPVLALAVVFAGTLLFAGACAREGGDRELHVVWDADVPSLDP
                     NERFDVVTATVASNVFEPLLRYDRQMSFEPCLAKGWEARDGAVWRFRLREGVSFHDGT
                     KLSAEDVAFSIERLRRRRKWDIHRYVSAITDVRVVDPLTVEIRSERPAGLLSVLSYVF
                     VLPKRSVEAAGEAEFFRAPAGTGPYRIASWKPGETLRLEAYPAYWGGPSAVSSVVFRV
                     EKDDEATWDEAKKLAPAILFAPTTTSWIRHRDDPAFRLVEQPSLAVHYLAFRVGGGPS
                     NPLSDIRVRRALRAAIDYEALLAALPAHGAFPASQFVPPAIVGFDPSLAVPEFVPGAA
                     RRMLAEAGFRQEKPLVFLTSAGGGAISRYLIEAFAAAGVSVREESVPPDEYESRLATC
                     DADAYLTGWICTTGDAGELFEGNFYRRGSTRNPCGYGCDEMDTAIEEIGRTLDPAVRR
                     DLLQSAMRRLVDDLPWVPLVVSYERHALTRGVEWTARADGQLDLRDVRIR"
     gene            11261..13219
                     /locus_tag="PLP50_12190"
     CDS             11261..13219
                     /locus_tag="PLP50_12190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPA52350.1"
                     /translation="MTPLRRSLRGRFALIFSALMGALLLVVSGTLILVRVRDHRDLLE
                     TYATGFAKATNNQLCNAWRLYYRSGAYKFRDIVRNTMGLNEDLHRLFILSVSGEVLFD
                     SMESPDLMLLPELPRRRLADPELVEVAGLQDDWTDRRELAGVGKVLMVSSPYFEEWGR
                     HPYSVLYIFKYDKIWQRVTSDLRSTLLLVLVALATVAAFASWMAGRVARPLLTLTAGV
                     RSFSEGKLESLPQVRTGDEIETLVEAFNRMAERIQQQVERLETANRELATLDRMKTDL
                     LANVSHELRTPLAAIRGYVEFIQEGQLGPTTDAQRKGLEVCLRNSERLTKTINMLLDF
                     SRMELGRVPIRTTPFQIGRLVAQIVAGIESEARKRNIRLVAVVDRSLRPVDGDRDRLT
                     QVLENLITNALKFTPDGGEVEVGARPAQAAGRVEVWVSDTGIGIPPEERARVFEKFYQ
                     TDGSATRRFGGIGLGLAIVKSILDAHGADIVVDGRPGGGTVFRFSLPVAETRTESGVF
                     HGLLPSDGDTAPVLAIDDDQDFLLLLKETLEKHGFAVRTATTAEEGLAAAREQAPLLV
                     VLDIRLPDRDGLDLLHELREDATTRQTPILIASVVDERLEGLRLGAVEYLVKPIDRGR
                     LVDAAIRALHSRGKRLPSGELPAMPQPS"
     gene            13295..13678
                     /locus_tag="PLP50_12195"
     CDS             13295..13678
                     /locus_tag="PLP50_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA52351.1"
                     /translation="MSQQKRTILIVDDEEDVLDLLQLVFETSGFLVRRASTGKAAVSS
                     AWEQPPDVVLLDVMMPEMDGWQVLRTLKGDERTRNVPVVMLSARAERRDKMIGLQEGA
                     EGYIAKPFSPAEVVREVQSFLERGS"
     gene            13675..>14743
                     /locus_tag="PLP50_12200"
     CDS             13675..>14743
                     /locus_tag="PLP50_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA52352.1"
                     /translation="MSSPEALPAAPFDDAASMRAEWLQNRARLHDVVTGLPTLPAVVD
                     QVRRHLEDHGSIALLAFSLATERQVEETWGWQLYDDLIRDFVSGLRAHAAVGGFSAGI
                     FCVPGVRSDEILCFVPVRPRNGSDQAPHEWLGALASRMDGFVSSFLAERLTSIDAYSS
                     HVGQALILFDPKVRVERAVYRGLNEARGEVFRHTANAEERGVEVLKGILADRRVVSLF
                     QPIHDLVEQEITGVEALTRGPAGSGLEDAERLFSLAEKAGLVIPLERLCRRRSLEEAA
                     RAGWDKLVFLNMSPAASQDSDFLEGRLVREVLELHLDPRQIVIEVTERTYAENQELFT
                     KVLGELRKEGFHVAVDDLGSGY"
BASE COUNT         1878 a         5528 c         5059 g         2078 t
ORIGIN      
        1 cggatcgacg acccgatcac gttcgacctc gatccgatcc ggaaggtgat cctttcgctc
       61 catcccgcag tccccgccgc cctcgtcccg cccttcacgc tcgacgtcca ggacgacctc
      121 tacgagaggg cggacgtccg gctgacgtgg cccgtcttcg cgggcggcag gatcgacgcc
      181 gcgcgggacg ccgcccgggc ccgcgaggcg gacgccgggg cgcagctccg tgccgtcggc
      241 gacgccctcg ccacggaggt cgtccgccgc tacaccgccg tccggctcgc cgagcgggcg
      301 cgcgcgatcc gcgccgaggt cctctccgcg ctcgacgagc acctccggaa cgcccgccgc
      361 ctcgaggagg aggggttcct ctcgcgcgcg gagcggctcc acgcggaggt cgcgcgaacc
      421 gaggcggagc gggagctgaa ggcctccggg cacgacctcg cgctcgcccg cgaggcgctc
      481 gcgaacgtcc tctcgctccc gggcgaggag gcggacggac tcgttacgac gacgcccctc
      541 ttcgtcccca cggagctgcc gcctcttccg gagctgaagg cggccgcccg cgacgcgaac
      601 ccggccctcg cgcgcctcga ggccgcggcc tcgctcgcga gcgccggtct cgccgccgag
      661 aagggccggt ggtaccccga ggtcgcgctc ttcggcgtac gcgagctcta caagccgggg
      721 ctgacgctcc tcgacccgac ctgggccgcc ggcctcagcg cgcgcatcac cctcttcgac
      781 gggttcgcgc gggagaaggg gatcgcggcg gcgcgggagc gcggcgcgcg cgtcgccgag
      841 ctggcgacgc gggcccgcag ggacgtcgag acgctcgtcg aaaagcgcta tcgcgaagcg
      901 cggaaggccc aggagcaggt cgaggcgttc ggggctgcgc tggagctcgc gcgcgagaac
      961 gtccgggtgc ggacccgggc gttcgaagag ggggtcgcga cgtctctcga cgtcgtcgac
     1021 gcgcgcctct ccctcgcgaa ggtggagctc ggccgcctcg cggcggctcg cgacctcgac
     1081 gtcgccctcg ccgagctcct cgaggcggcc gggaggactg aatcgtacga agcgctccgc
     1141 gcggacgggg cgggggacgt ggagaagtga acgaacgaac gaagggactc ctcgcgctcg
     1201 ggggcggcgt cgccgtcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1321 agtgaacgaa cgaacgaagg gactcctcgc gctcgggggc ggcgtcgccg tccttctgct
     1381 cctcgggctc gccgccctcc tcctctcgcg ccccggcccc gagacgctcc agggggaggc
     1441 cgacgcgacg aaggtcgacg tcgccgcgaa ggtcgccggg cgcctcgcca cgatcgacgt
     1501 gaaggagggc gacacggtcg cgcgaggcgc tctcatcgcc acgatcgaga gccccgagat
     1561 ccgcgcgaag ctggaccagg ccgaagccgc gaaggcgggt gccgtcgcgc agcgcgacaa
     1621 ggccgaccgc ggcgcccgcg aggaggagat ccgcgcggcg aagagccagt gggaccgggc
     1681 gaagcacgcc gccgagctcg ccgagacgac gttcggacgg ctcgagcgcc tcgcaaggga
     1741 cggcgtcgtc ccgaggcagc ggcgggacga ggctgaggcg cagtggaaga cctcgcgcga
     1801 cgcggccgag gcggcgaagg ccgtttacga catggcggtc gcgggggcgc ggcgcgagga
     1861 ccgggccgcc gcggcggcgc tcgtcacccg cgcggaggga gccgttgacg aggtaaaggc
     1921 gttcctcgac gagacgaagg tcgtctcccc ggccgcgggc gaggtctacc gccggaacgc
     1981 cgagccgggc gagatcgtcc cggccggttt cccgatcgtg acgctcgtcg acctgtcgga
     2041 cgtctgggtg acgttccagg tccgcgagga ccgcctcgcg cggctgaaga tggggacgag
     2101 cgtcccggtg cgcttccccg ccctcggcgg cgaggaggtc ccgctgacgg tcacgttcgt
     2161 cgcgccgcag ggggacttcg cgacgtggcg ggcgacgagc gcgcaggggg gcttcgacct
     2221 gaagacgttc gaggtccgcg cccgtccggc gaagccgctg cccggcctcc gtcccgggat
     2281 gagcgccgtc gtcacgtggc cccgctaggg agcgcaccgg gcgccctccg cgcggcgagg
     2341 cgggaggcgg agcggctcgt gacgagccgc cacgccctcg tcctcctcgt cctcctgccg
     2401 ctcgcgggct tcgccctcgc gacggcgatc ttctggaacc ggcttcccga ggagctcccg
     2461 gtcgcggtcg tcgacgccga ccgctcgctc ctctcgcgcc gcctcctccg gatgctcgac
     2521 gccacgaagt cgatgcgcgt cgccggccct tacgcgacgg gcggcgaggc gctcgacgag
     2581 gtcctggcgg gacgcgccta cggcgtcgtc tcggtgccgc ggcacttcga gcgcgacgtg
     2641 aagcggggcg ccgcgccgaa gatcgtcctc cactacgacg cgctgacgat gatccccggg
     2701 agcatcctga agcgggacgt gagggcggtc gtcgggactc tctcggccgg cgtcgagatc
     2761 caggcccgcc gggccctggg cgagacgccc gacgtcgcgg gggcgcgttt cgagccggtg
     2821 aggtcgctcc gcggcgcgct cttcaatccc ggcctcgact accgcgcctt cctcctcccg
     2881 gcggtcctcg ccacgctcct gacgatcttc gtcctcgtcg ccgccgtcca cgccctcggc
     2941 gtcgagctgc gcgacgggac ggccgcggag tggctcgcct cgggcgggag cgcgacgcgc
     3001 gccgtcctcg gcaagctcct cccgcagtct ctctggttca cggcgctcgg cctcgtcgcc
     3061 gtcgccgtcc cgttccgcct cctcgaggtg ccgctccgcg ggagcgcgcc cctcctcgcc
     3121 ctcgcgacgc tcctcctcgt cctcggctac caggccgtcg cgctcctcct cgtcgcgttc
     3181 agcgcgaacc tccgcttcgc ctcgagcgcg gcggcgttct acggcgggcc cgccttcgcg
     3241 ttcaccggga tcaccttccc gacgttcggg atgccgctcg cagcgcagct ctggggggcg
     3301 ctcctccctc tcacccactt cctcgccctc ctcctcgccc aggcgatgcg cggcgccccc
     3361 gcctcggccg ccctcgggcc gctcggggcg ctcctcgcgt tcgtcctcgt cctcccgcct
     3421 ctcagtctgt ggcgcatgcg gcgactcatg agcgacccag cctattgggg ccggaagtga
     3481 ggagcgtcct tcgggccttc cgcgaggagg tgtcgggcgc gctccgcgac ccgggcgccc
     3541 tcctcgtcct cgtcggcgcg atcgcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnntcg ggcgcgctcc gcgacccggg cgccctcctc gtcctcgtcg gcgcgatcgc
     3721 gctctacgcc ttcttctacc cgacccccta ccggcgcgag gtgctcaagg aggtcccgat
     3781 cgccgtcgtc gacctcgacc ggacgcccct ctcccgccag ctcgtccgct tcgccgacgc
     3841 tcacgagctc gtccgcgtcg ccgcgcacgc cgggagcctc gcccaggccg aggcgctcgt
     3901 ccgcgacggg aaggcgggcg gcgtcctcgg catcccggag ggcttcgagc gcgaggtgaa
     3961 gcgcggcgcg ccggcgtcgg tcggcgtcct cgccgacgcg acctacttcc tcgtctaccg
     4021 gcaggtcctc accggcctcg cggagtcggt cgggacgctc tcggccggaa tcgagatccg
     4081 ccggctgacg gcgcagggaa tgcccgaggc gaaggcccgg agcttccgcg acccggtccc
     4141 gctcgtctcg cgccccctct tcaacccggc cgaggggtac gcgacgtacg tcgtcccgcc
     4201 cgtcctgatc ctgatcctcc agcagacgct cctgatcggg atcggcctgc gcggcccggc
     4261 cgacctcggc cggctcggct ccggcccgct cgtagcccga gccgcggcct cggtgacggg
     4321 gaggacgctc gcgttcgtcg ccctctacct cgtccatgcc ctcttctact tcggcgtcgt
     4381 cgcgcgcctc ttcgatttcc ccgtccgcgg gagcgcagcg gccgcggcgg gcctcgtcct
     4441 ccccttcctc ctcgcgacga gcttcctcgg cctcgccctc tccgtcctct tccgccggcg
     4501 cgaggcggcg atccagacgc tcctcttcac gtccctcccg gcgatcttcc tggccggatt
     4561 ctcctggcct cccgctgctc tccccgcctg gctgcggttc gcctcgcagg cgatcccctc
     4621 gacgagcgcg atcccggcgc tcctccgcgt gaaccaggcg ggcgcctccc tcgcgcacgt
     4681 gcggcacgag ctcgcgctcc tctgggggct cgcggtcgtc ttcttcgtcc tcgccgtcct
     4741 cgcgcaggcc ggccgggagg cggcggtccg gcgtcagtac cgggcggcga cgaagccgta
     4801 gaagttgtcg aggaagtcgt cgagcgggag gacgccggcc tgcggcaccg cgcggcgccc
     4861 ctctccccac gtgaagatcc ggagcgagac ggtcgtcgcc ggcttccctt cgatcatggt
     4921 ggccccgaac gtgacgcgcc cggtctggac gacccagtgg tcggcggtcg ccgacccgtt
     4981 cgcctgcgtc gaggggctca gcatgttgga gtggatgaaa aggcagacgc ggaagccctt
     5041 ccggaagaag tcgtcggccc tccggaggca tggctcgtcc ttgtcgacga tcgcccgcgc
     5101 gtcgttgacg acctcggtat agcccgcctt ccggaaccag ccctcgagct ctcccggaag
     5161 cgtgatcccg gcgagcgcgt cggagtcctc ctggtagtcg aagaaccagt tctccgagtc
     5221 ccgaaggctc gcgagggcga tccagtctga ggcctcgacg ttccgccccg ggtcgaaggc
     5281 cttcaggtcc gtgccgggcc gcacgtcgat gcggccgatc cggccctggc cggtctccca
     5341 gagcgccgtc acgtacctca cgtacgcctc cgggtcgcgc gtggcgacgt cgaagaggag
     5401 ggacgccggc ccgcagaggc tcgaggtccc ctggtcgatg gcgctcggcg cccagacgcg
     5461 cacgcgcagg ccgtcggcga cccgcttccg cgagatcgtg gtgaaagcgc ccccgccgat
     5521 ccgagcggcg aaccggtcca cgagctcctc ggcgcgctgc tgaggcgtcg ccgatcgacc
     5581 cttcgcgtcc gcgacgtcag cgggtgcgac tcgcgcgccg cggccgatcg gaccgggaaa
     5641 gacgaaggcg cgacccagca gcccctcggg gaccgggtcg tgacgggaat ccgttccgcg
     5701 gggaccgggg acattcgatc cagccatcag tacctcggca tcaggtcgtc gtcaccgacg
     5761 tagcaggtga tcctgtcggg gaaggaaggg ccgagctccg cgtcgagcca gaggcgttct
     5821 cccctctgct cgagccgccc ctcgacggac ggcgcggccg ggtcgctcgt caggcaggcg
     5881 accgagcgcg ggtgtccgaa ccagcgctgc cagaggcccg gggcggggcg ctcgacgccg
     5941 atcgcgagcc ggatccggaa accaggcccc ctcgtccact cgcgaagggg ccagagctcg
     6001 taccggaagc gctggacgaa gcgtccggcg cggcgggagg aatgcccgac ctcttcgccc
     6061 ccgagcggct ggcggtaccg gaccgtgatc gtgttcgcgc cttcgtggag ctcggcttcg
     6121 aacgcggcgc ggtggagcgg cggctccccc ggccccggct ccgggaggct gcgccgctcc
     6181 tcggcccgga acggtctcgc cggggcgacg ctcacctctc ccgtcccccc gttcgatcgg
     6241 gccgtcacgt cgcccgcgac cgggaggacc atctcgagcc ggacacgctc cggtgcccca
     6301 gccgtgacgt cgtactcggc cgtgacgtcg cacgcctcgg ctccgcagag aagcgtgagc
     6361 cgctcgcgct ccacgacgag ctccttcgcg ggagccgcga gcgcggtcga gggggcttcg
     6421 gggactcgtc gcggcgcacg caggttcgcc gccgccggga acgccgccag gagcgcgagc
     6481 gcggcggcca gcgctcgccg gcgcgcgaag ccgccgtcgg cccgacgtgt cgtcccgtcg
     6541 acgttgtcct gcacgcgatc agcctacgcc cgctcccgga ccggctcaag aagcgcgcga
     6601 gcgccggggc gtgacccgcg tcacgacgaa ggcgtgcgga gaagcgaaag cgcctcggcg
     6661 cgtcgctcga ggctcgcctt cccctggaag agctttccgg acccgccgcc gcgcccgtcc
     6721 agccgcgccg ccacggcgga gccgagagcc cggacgtcgg ccgaggaccc cgcacccgcc
     6781 gcgaggacga agaccccggc cccgtccttc tcgcccgtca ggaaggcgag cccaccgacg
     6841 ggcagctccg tgaaccgcct cgcgacccga ccgacgaagg cgaggtcccg tccctcgaag
     6901 tgcgcgtcga cgacgggagc tcccgccgcc gcgagcgtct ccgccttcgc ctcggcgagc
     6961 tcctcctccg cgccgcgcag cgccttctgc gcctcggcca gctgcgccag cttcgcggcg
     7021 accgcttcgg cgaggcccgg gtcgggcgcg ccgaggaggg cgcggaggcg cccgttgcgc
     7081 tcctccgcct cgccgagccg cctccggaca cggctgccgg cgacgaagaa gagtcgcgtc
     7141 ccgccgcgca gcttctccgt gccgaggagc ttcagggcct cgagctccgc cgtcgaccgg
     7201 aggtgggtgc caccgcaggt gttgaggtcg acccccgcga tctccacgag acgcacgtcg
     7261 ccgacgtgcc cctccggcag gccgcgcgag cggaccccga ggcccggcag ctcctccgcg
     7321 cggacgcgcc gcgccgtcac cgggagggcc gcccggatgg cggaggccac cgcgtcctcg
     7381 aggcgctccg cgtcggccgg gacgatcgag gggacggcga gctcgacgtc gcacgtctcc
     7441 ggtccgaggt ggaacgccgt cgtctcccat ccgaagcgat cggccgccac cgcggtgagg
     7501 agatgctggc ccgtgtgctg ctgcatgtgg tcgagccgcc tcgcccagtc gaggcggacg
     7561 accgtcggcc cttccgggac ttcggtttcg agaacgtggc gcacccggcc gtcgaccgtc
     7621 cggacgtcga cgacggggac ctcgccgagg aatccgtgat ccgcgggctg cccgcccccc
     7681 tcgggataga ggacggtgtc gtcgaggacg gcccagggcc gtcccccttc gctccccgtc
     7741 gcgacgacgc gaacggggag ctcggtgagg tacgggtcgc gttcgtacgc ctgcggagcg
     7801 ctcatggacg aagattacct tcgcccggtc gctgcgggcc cgctccccgc cggcgccccg
     7861 gccttcgggc gctcctcagc cggaaggctg gccgtccttc aggagggtcg agagctcgcg
     7921 gaaccggggg tggttcttgt cgcgggccca ctcgaagccg accgattcgt ggttcgtgat
     7981 cgcggccccc gcagcggcca tccgctcgag cgcgtgacgc cggtactcct cgccccggcc
     8041 gctcaccgcg tcccacgaga cgtggacctg gttcccgtgg tggatcagct cgactacggt
     8101 ctgcaggacg cacacgtgcg cctcgatccc cgccacgagg acctgtcgcg tcgcgcgcgc
     8161 ccggggccgg agctcgtcga gcgcccgaag gaacgccggc tcgccgcagc agccgaacga
     8221 cgtcttgtcg acgtacctct tcgggaccgt gagcgcgtcg aacccggcgc ggatctccgc
     8281 ctgggtcggc ccgagcccct tcggatactg ctcggtcagg acgaccggca ctccgaaaag
     8341 ctcggcgagc ttcatcagcc ggagggtcgc ctcgacgacg agccagggtc ggtggatcac
     8401 gtccatcagc ttcccctgca ggtcgatcac gacgaggacg ctgcgtcccg agtccaggag
     8461 ctccatggaa tcatctcccc gccggctcgg cgccggccgt cttcggggtc gcggactccc
     8521 gccgcggccc cccgcttctc accaccgata aatcgcgacc gccgcgacct catgcggcat
     8581 cttcgcggca tcgaggacga agacctcacc cccgcgggcg aggaccgtct ccgcgaggtc
     8641 gtcgaggacg tcgtcgtcct gaggccccga ctgccccgcg tggagcgtga cctcccccgt
     8701 ctcgcggtcc acccggccga agagcgtctt cccgccggcg acgacgagcc gccggacgcg
     8761 tcccgcgacc gccgcctcgg cgacggtcgt cagcacgtcg ctgccgaggc cccggtcctg
     8821 cgccttgagg aactcctcga cgagccgggc gttcctcgcc tcgagggcgc gacgtgcggc
     8881 cgggaggacg cgctcgtgga gctcgtcgac ggcgacgtcg tccaccgcgc cgtcgacccc
     8941 ttcggggtgg accgccggat gcgtgcagac ctcccggaac gcggggagga cgtttccggg
     9001 ccccgcgagc aggatcggcg tcgcgcccgg gagcgcttcc cggagcgcgg cgtccgcggc
     9061 gcggaagcgc cggagcgtct cctcgcgccg gcctccgtcc ccttcggcgg gcgccttgcc
     9121 gggtccgccc ctcgcccccc ttccgccgcc gttgcgggcc ccgcccgtcg cggtcggctc
     9181 gagcggtgcc ggcaggcccg gcagggcgcg cgggacgagc cccctttccg acccgtcgta
     9241 cagcgcgacc cggcgggtac cgatcgcgag gacgtggtag cggttccggt cctggaggct
     9301 tcggaggagc ggcttgacgt ggaagctgtc cgcgaccacg gccctctccg gcggagaggg
     9361 ccagaccctg taggtctcga ggaccccgtc ggcccggaac gccgcgagcc cctccggtcc
     9421 cggggtgaag acgtcgacgg cctccgccag cccggcgagc ggcgcgatca gctctcggat
     9481 ccgtttcgcg tcgtaccgtt ccgcgaggag cgcctcggcc tgccgcacca ggttccggaa
     9541 ccggaccggc gccatcgcca gctcggcccc gtgccgaggc agcggcagga agagagaaat
     9601 gcacggctcc gtcctcggag cgagaaggtt ccggaggtcg tccggcgtcg gcttcttcgg
     9661 catgtgcggt cctccctcgt gcgggagaca acgatacgat ctccggcggg attcccgcca
     9721 cccgtctgac gccgggagcc cggggcgggt aacatgaccc cgtgcggagg ccggttctcg
     9781 cgttggccgt cgtgttcgcc gggacgcttc tgttcgccgg ggcctgtgcc cgtgagggag
     9841 gcgatcgcga gctgcacgtc gtgtgggacg cggacgtccc gtctctcgac ccgaacgagc
     9901 gattcgacgt cgtgacggcg accgtcgcgt cgaacgtctt cgagccgctc ctccgctacg
     9961 accggcagat gtcgttcgag ccctgcctcg cgaaggggtg ggaggcccgc gacggcgccg
    10021 tctggcgctt ccgcctccgc gagggcgttt cgttccacga tggcacgaag ctctccgccg
    10081 aggacgtcgc gttctcaatc gagcgcctgc ggcgccggcg gaaatgggac atccaccgct
    10141 acgtctccgc gatcaccgac gtgagggtcg tcgacccgct cacggtcgag atccgttcgg
    10201 agcgtcccgc ggggctcctc tcggtcctct cgtacgtctt cgtgctgccg aagcggtccg
    10261 tcgaggcggc cggagaggcc gagttcttcc gcgcccccgc cggcaccggg ccgtaccgca
    10321 tcgcctcctg gaagcccggg gagacgcttc ggctcgaggc gtacccggcc tattggggag
    10381 gtccctccgc ggtgtcctcg gtcgtcttcc gggtcgagaa ggacgacgag gcgacgtggg
    10441 acgaggcgaa gaagctcgcg cccgccatcc tcttcgcgcc cacgacgacg agctggatcc
    10501 gtcaccggga cgacccggcg ttccggctcg tcgagcagcc gagcctggcc gtccactacc
    10561 tggcgttccg cgtcggcgga ggcccgtcga acccgctttc cgacatccgc gtccgccgcg
    10621 cgcttcgggc cgcgatcgac tacgaggcgc tcctcgccgc ccttcccgcg cacggcgcct
    10681 tccccgccag tcagttcgtc cctcccgcga tcgtcggctt cgacccgtcc ctcgccgtcc
    10741 cggagttcgt ccccggcgcc gcgcgccgga tgctcgcgga ggcgggcttc cggcaggaga
    10801 agcccctcgt cttcctcacc tcagccggcg gcggagccat ctcccggtat ctcatcgagg
    10861 cgttcgcggc ggcgggcgtc tcggtccggg aggaatccgt ccctccggac gagtacgagt
    10921 cgcggctcgc cacctgcgac gccgacgcct acctcacggg ctggatctgc acgaccgggg
    10981 acgcgggcga gctcttcgag gggaacttct acagaagagg ctcgacgcgg aatccctgcg
    11041 gctacggctg cgacgagatg gacaccgcca tcgaggagat cggccggacc ctcgacccgg
    11101 ccgtccggag ggacctgctc cagtccgcga tgaggaggct cgtcgatgac ctcccgtggg
    11161 tgcccctcgt cgtcagctac gagcggcacg cgctcacccg cggcgtcgag tggacggcgc
    11221 gggccgacgg gcagctcgac ctgcgcgacg tgaggatccg gtgacgccgc tccgccgctc
    11281 gctccggggc cggttcgccc tgatcttctc ggccctgatg ggagcgctcc tgctcgtcgt
    11341 ctcggggacg ctcatcctcg tccgggtccg ggaccacagg gacctcctgg agacctacgc
    11401 caccgggttc gcgaaggcga cgaacaacca gctctgcaac gcctggcgcc tctactaccg
    11461 ttccggcgcg tacaagttcc gcgacatcgt ccggaacacg atgggcctca acgaggacct
    11521 tcaccgactc ttcatcctct ccgtctcggg cgaggtcctc ttcgattcca tggagagccc
    11581 cgacctcatg ctcctgccgg agctcccccg ccggcgcctc gccgaccccg agctcgtcga
    11641 ggtcgcgggc ctccaggacg actggaccga ccgccgcgag ctcgcgggcg tcggaaaggt
    11701 cctcatggtc tcgagcccgt acttcgagga gtggggacgc cacccgtact cggtgctcta
    11761 catcttcaag tacgacaaga tctggcagcg cgtcacctcg gacctcaggt ccacgctcct
    11821 cctcgtcctg gtcgcgctcg ccaccgtcgc cgcgttcgcg tcgtggatgg cgggccgggt
    11881 cgcccggccc ctcctgacgc tgacggccgg ggtccgatcc ttctcggagg ggaagctcga
    11941 gtccctgccg caggtgagga ccggcgacga gatcgagacg ctcgtcgagg cgttcaaccg
    12001 gatggcggag cggatccagc agcaggtcga gcggctcgag accgcgaacc gcgagctcgc
    12061 gacgctcgac cggatgaaga ccgacctcct cgcgaacgtc tcccacgagc tgcggacccc
    12121 gctcgccgcg attcgcggct acgtggagtt catccaggaa gggcagctcg gcccgacgac
    12181 ggacgcgcag cgcaagggcc tcgaggtctg cctgcgcaac tccgagcggc tgacgaagac
    12241 gatcaacatg ctcctcgact tctcgcgcat ggagctcggc cgcgtcccga tccggacgac
    12301 ccccttccag atcggacgcc tcgtcgccca gatcgtcgcc gggatcgagt cggaggcgcg
    12361 aaagcggaac atccgcctcg tggccgtggt cgaccggagc ctcaggccgg tcgacgggga
    12421 ccgcgaccgc ctcacccagg tcctcgagaa cctgatcacg aacgcgctca agttcacgcc
    12481 cgacggcggc gaggtcgagg tgggcgcccg gcccgcccag gcggccggcc gcgtcgaggt
    12541 ctgggtctcc gacaccggga tcgggatacc gcccgaggag agggcccggg tcttcgagaa
    12601 gttctaccag accgacggct ccgcgacgcg gaggttcggc ggcatcggcc tcggcctcgc
    12661 gatcgtcaag tcgatcctcg acgcccacgg cgccgacatc gtcgtcgacg gccggcccgg
    12721 gggcggaacg gtcttccgtt tctcgctccc cgtcgccgag acgcgcaccg agtcgggcgt
    12781 tttccacggg ctcctcccct cggacggcga cacggcgccc gtcctcgcga tcgacgacga
    12841 ccaggacttc ctgctcctcc tcaaggagac gctcgagaag cacggtttcg cggtccggac
    12901 cgcgacgacg gccgaggaag ggctggccgc ggcccgggag caggccccgc tcctcgtcgt
    12961 cctcgacatc cgcctgcccg accgcgacgg cctcgacctg ctgcacgagc tgcgcgagga
    13021 cgccacgacg cgccagaccc cgatcctgat cgcgtccgtc gtcgacgaac ggctcgaggg
    13081 cctccggctc ggcgccgtcg agtacctcgt caagccgatc gaccgcggca ggctcgtcga
    13141 cgcggcgatc cgcgcgctcc actcgcgcgg gaagcggctc ccttcgggcg agctcccggc
    13201 gatgccgcag ccctcctgag cgtcccgaga agcctccgat tgctctaaga tcgagtcggg
    13261 cgccagagcc ccggacgcgg cgaggaggga tccgatgtcc cagcagaaac ggacaatcct
    13321 gatcgtcgac gacgaggagg acgtcctcga cctgctgcag ctcgtcttcg agacgagcgg
    13381 tttcctcgtg cgccgggcct ccacgggcaa ggccgccgtg tcgagcgcct gggagcagcc
    13441 gccggacgtc gtcctcctcg acgtgatgat gccggagatg gacggctggc aggtgctccg
    13501 caccctcaag ggcgacgaga ggacccggaa cgtcccggtc gtcatgctct ccgcgcgcgc
    13561 cgaacggcgg gacaagatga tcggcctcca ggagggtgcc gagggctaca tcgccaagcc
    13621 cttctccccg gccgaggtcg tccgggaggt ccagagcttc ctcgagcgag ggtcgtgagc
    13681 tctcccgagg cgcttccggc cgcgcccttc gacgacgccg cctcgatgcg ggcggagtgg
    13741 ctccagaacc gggcccgcct gcacgacgtc gtgacgggcc tgccgaccct ccccgccgtc
    13801 gtcgaccagg ttcgtcgtca cctcgaggac cacggctcga tcgccctgct cgcgttctcg
    13861 ctcgccacgg agaggcaggt cgaggagacg tggggctggc agctctacga cgacctcata
    13921 cgggacttcg tctccggcct tcgcgcccat gccgcggtgg gcggcttctc cgccgggatc
    13981 ttctgcgtcc ccggcgtccg gagcgacgag atcctctgct tcgtcccggt gaggccgcgg
    14041 aacggctccg atcaggcgcc ccacgagtgg ctcggagcgc tcgcgtcccg gatggacggc
    14101 ttcgtctcct cgttcctcgc cgagcggctg acctcgatcg acgcctattc ctcccacgtc
    14161 gggcaggcgc tgatcctctt cgacccgaag gtccgggtcg agcgcgcggt ctaccgggga
    14221 ctgaacgagg cgcgaggcga ggtcttccgg cacacggcga acgccgagga acgcggcgtc
    14281 gaggtcctga aggggatcct cgccgatcgt cgggtcgtct cgctcttcca gccgatccac
    14341 gacctcgtcg agcaggagat caccggcgtc gaggccctta cgcgggggcc cgccggaagc
    14401 gggctcgaag acgccgagcg gctcttctcg ctcgccgaga aggcgggcct cgtcatcccg
    14461 ctcgagcggc tctgccggcg gcgctccctc gaggaggccg cccgggcggg ctgggacaag
    14521 ctcgtcttcc tcaacatgtc acccgcggcg tcccaggatt ccgacttcct cgagggccgg
    14581 ctcgtccgcg aggtcctcga gctgcacctc gacccgagac agatcgtcat cgaggtcacc
    14641 gagcggacct acgcggagaa ccaggagctc ttcacgaagg tcctcggcga gctccgcaag
    14701 gaggggttcc acgtcgccgt cgacgacctc ggctccggct act
//
LOCUS       DAOONL010000085        14570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4436_length_14570_cov_10.382845, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000085 DAOONL010000000
VERSION     DAOONL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_14570_cov_10.382845"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PLP50_12205"
     CDS             complement(<1..1816)
                     /locus_tag="PLP50_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPA52353.1"
                     /translation="MNKLVGTLASLKLAVVLLVVLLIGLSAGTILESRTDAVTAGRLV
                     YYSWWFLGLQGLFAVNVACSIATHFPWGKQRIGFVTTHASLLLIFVGAALTYFGKTEG
                     QLGLWEGQASSTVEHVDEKGTVLAKHDLPFSVKLDDFVLETYQGTMRPSGFASQVVVT
                     DLDTGKSFPTRIFMNTPLHHRGWSLFQSSYQQQGGREATVLSVSKDPGQLVVFAGYAT
                     LVLGMILVLLTRVSQARERERSRSDLEAKVAQGGMSLPTMGKALGAVLALAGLAGPTS
                     AQTPAEVEALKRLPVQHDGRVMPLDTLARETVWNVTGSRSWNGQDPAATFVGWLLDPK
                     GASSAPAVRVTRDLAAAAGLDPAARQASFHQLVSNPTVMRLIESAGAAAQHDQPRLGV
                     LKDAEKLEERLNALYAVLQREVVRPIPAPSDPKARWDLPLEVSTPALLALANGPRLSG
                     WPTKEKVEREVLYNRVNPSRIAWLVLLASLVVSIAAWAQSSKGLDWASFGLLVVGFGA
                     MTWGIGMRWAAGERIPAANMYESLLFLGWGVGLFAVIAYVLIRNRTVVLNAAAMSALT
                     MALTDLLPMDRFIHPIAPVLAGTPWLAIHVPIIMVGYAV"
     gene            complement(2023..3945)
                     /locus_tag="PLP50_12210"
     CDS             complement(2023..3945)
                     /locus_tag="PLP50_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPA52354.1"
                     /translation="MAESSSLAPLSFWRSPAVRRVLAGGLLLAGGLALEQLGFGHAWV
                     VPLYLASILLAGADWVRHGWQELTHCHEVGIEVLMAAAAVGAAALGLWDEAAALVFLY
                     GMAEAVEHLTYDRAKRAIERLLDLVPAEATVLRDGGEVVVPASLLQRGDRIRVRPGES
                     IPTDGLVEEGESAVDESSLTGESIPIEKKPGERLFAGTLNRTGSLVFRATATAAENSV
                     SRLVKLVEAAQKSKGRGQQLVERFTNAYSPLVLGAAVLLLLVPLVSGGAFATWARYAV
                     VLLVAAAPCALAMSTPVAFAAGISAAGRHGILIKGGRYLEVLARVRTVAFDKTGTLTR
                     GRPEVVVVEPARGTTEWELLWKAASLEVHSEHPLAAAVVRHAHSKGVAAFQEVTRFRA
                     LPGSGVQGYVDGECIQVVQPEAGGVPFGDVADRLAELAGAGVTPAIVLSGGRFLGLLG
                     FRDEPRPEASTAIRHIRKAGVSRLVVLSGDRPLVAENVARSVGVDDARGGLRPEEKVD
                     AVRALSAESGPVAMVGDGINDAPALAAAEVGIAMGALGSDASIEAADVALMADSLEHL
                     DEAFLIGRKVRTIVLQNVVFSIVVLAVLIPVALTGAITITMTVVAHEAAELLAVANGL
                     RALKGGRHHTPTVEAT"
     gene            complement(3938..4312)
                     /locus_tag="PLP50_12215"
     CDS             complement(3938..4312)
                     /locus_tag="PLP50_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPA52355.1"
                     /translation="MHGVVAVIQPFMLEQVVDALRLVPNFPGMSVSEVRGFGRRYAHA
                     PHWGERTEVEPFEEKLRIDIYCRDAELKAIVDTIRRAAHTGNPGDGKIFVSPLTRVVG
                     IRTGEEGPEVLLCGYPSDNAHG"
     gene            complement(4317..7421)
                     /locus_tag="PLP50_12220"
     CDS             complement(4317..7421)
                     /locus_tag="PLP50_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPA52356.1"
                     /translation="MLRRLVDLSLENRAIVLLAAVLLIAGGAYSLTQLPIDAQPDITN
                     VQVQVLTKAPALGPVEMEQFVTYPVEAAMNGLPKLQEIRSISRYGLSAVTIVFQDGVD
                     VYFARQLVSERLVQAREAIPEGLGTPELGPVTTGLGDVFQFTVEGDGVSAMERRTILD
                     WMIAPRLRAVPGVTEVNTWGGLPKQYQVVVDPAKLRAHGLSLKEVFEAVERGNANAGG
                     GYIEHNREQYIVRGEGLVSSLSDIEKIVLKAAPEGTPVTVGAIAQVREGSMLRIGVAT
                     ADGHGETVIGLVQMLAGENALQVATRAREAVEELQPTLPKGIRIVPYYDRAALVRRVI
                     RTVETNLLEGSILVIAVLFAFLGNVRAALIVASAIPLSMLLAFTGMVESRISANLMSL
                     GAIDFGLIVDGAVVLIENVVRRLSEPEGRDKTVRQLTAEAAHEVVRPITFGIGIIILV
                     YLPILSLQGVEGKMFKPMAWTVVFALAGSLLLTLTLTPVLASLFLKKTGHDHEPRFVG
                     KLRVLYLRGLDVCLSRRAPVVIAAVVLVAVGAAVASTLGGEFLPRLDEGDLSISAVRP
                     PSVGISEVAASTGRIERVLKRFPEVITVVSRSGSPELATDVMGIELGDVFVMLKPKDE
                     WTSAKSKGELVEKMAEALGETVPGVGFSFQQPIEMRFNELIAGVRSDVGIKLFGDDLE
                     LLRQKGEEIARVVATVPGAADVKAEQTAGLPVLRVKVDRDRCARYGISVGDVLDTVEA
                     ARAGKIVGTVFEGQRRFSLAVRFDDSAAQTIEGLRNVPVAAPGGALVPLGQLADIVLD
                     TGPSQISREAVKRRVVVEVNVRGRDVASFVTETKAKLQSAVKLPDGYYIRWGGQFENL
                     ERASRRLMVVVPLALALIFAMLYFTFGALKPAVLIYLNVPLAATGGVIALALRGLPFS
                     IAAAVGFIALFGVAVLNGVVLMTQIRDLEARSHLDIADVLRKACGLRLRPVLMTALVA
                     SLGFVPMALATGSGAEVQRPLATVVIGGLVTSTILTLFILPTIYSFFGRAGRAAKTEV
                     THGQ"
     gene            complement(7423..8658)
                     /locus_tag="PLP50_12225"
     CDS             complement(7423..8658)
                     /locus_tag="PLP50_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52357.1"
                     /translation="MNRKPTILLSGLALSAAVALAGCGKGRGAAPAPEAPAKTAEKVE
                     EPATPQRVHLTAAAIAEAGITTWKVQIVDLSHILSLTGSVGFDENRLLLVAANVKGRV
                     TSIPVDLGARVKPGDSLLVIESVDLGRTREEFVTELSSFNVSASAYERARKLVEANAI
                     SAGEFQAREGDYLAKKTAVASAERTLHLYGDSEETIARLRANAVDHRLSAADGATLTL
                     RAPFAGRVIDRKVTSGALFEALQPLMTIADLGSVWVFLNAYEKDLALLHEGLSVTLRT
                     DAYPQETFRGRVDFLGSVVDPQTRSVRVRATVENRSEKLRPGLFVTAQVDVPKPQSEA
                     HPIVAVPQSAIQRLEGRSTVFVQVEPGVFQRHYVEVGHSFEGFTEILAGIKAGDVVAT
                     EGSFVLKSEFAKASLVEED"
     gene            complement(8655..9827)
                     /locus_tag="PLP50_12230"
     CDS             complement(8655..9827)
                     /locus_tag="PLP50_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA52358.1"
                     /translation="MDDYVRLVLQSHPSAQASLALDASASAESKAARLLPDPTLELSL
                     GEGRAADGTGSTKTEKGFSVSQSIPWLPARSASIEAADHEARALRAEGLATRWGLELD
                     AQVTYERLLFARAQVEVGRASAEDARSLLDLMTRRASLGEAREVDRIKARVEWLRQDR
                     QLSAADREELAAEGIMRTLAGEPLPTPLRLAGELPRHPDGMRALLDAHPDALERSPAL
                     IRARAEAARASSLLSSARRGRVPDLGFSFFRQNELDREAWGGALGLTLPLWNARRGDV
                     ARAEAESRLRAADAERVRLALLSDLATRRRDVETLAVQVASLEGDFLPSAAESVRLAR
                     LLFEEGETSLLDLLDAQRTAREARREEIRARFELATAIAELRRLLGPNDDTTGDSK"
     gene            complement(10727..12115)
                     /gene="merA"
                     /locus_tag="PLP50_12235"
                     /pseudo
     CDS             complement(10727..12115)
                     /gene="merA"
                     /locus_tag="PLP50_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
     gene            complement(12196..12393)
                     /locus_tag="PLP50_12240"
     CDS             complement(12196..12393)
                     /locus_tag="PLP50_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPA52359.1"
                     /translation="MSCPTCPITVRKALLAVHGVTAAEVSLERKTVLVTFDATKTTVG
                     ALTRATVDAGYPSSLQGEVTR"
     gene            complement(12520..12894)
                     /locus_tag="PLP50_12245"
                     /pseudo
     CDS             complement(12520..12894)
                     /locus_tag="PLP50_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
     gene            12966..13409
                     /locus_tag="PLP50_12250"
     CDS             12966..13409
                     /locus_tag="PLP50_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPA52360.1"
                     /translation="MTIGRLAEAAGVHVETVRYYQRIGLFPEPTRAYGSARRYGPADA
                     AHLRFIKRAQGLGFALDEVRLLLQLAVGEHCAETRMLAERKLALVEEKLRDLSEIQSV
                     LRGLVRACGAGAPGCGCPIIDRLAGEGRSTLPARQTSASGRDRSS"
     gene            13419..13799
                     /locus_tag="PLP50_12255"
     CDS             13419..13799
                     /locus_tag="PLP50_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPA52361.1"
                     /translation="MAFDELLADRVRSILSRRSDVVERKMFGGIAFMVSGSMACGVVA
                     SDLMVRVGAERYKAALARAHARPMDFTGRPLRGMVYVAAGGLATESVLEAWVAEAVSV
                     ATSASPAPTLSRATKKPPGRKRSR"
     gene            complement(14020..14292)
                     /locus_tag="PLP50_12260"
     CDS             complement(14020..14292)
                     /locus_tag="PLP50_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPA52362.1"
                     /translation="MGLDQPGFLADERTSDAVVRNLEILGAAATHVPDDFKASEPDLE
                     WPRIVGLRNRVVHEYFAVDLELVWRIVVSDLPSVASRLRTLRNFLA"
BASE COUNT         2371 a         4768 c         5245 g         2186 t
ORIGIN      
        1 ggacggcgta gccgaccatg atgatcggga cgtggatcgc gagccacggc gtccccgcga
       61 gaaccggagc gatcggatgg atgaagcggt ccatcgggag gaggtcggtg agcgccatcg
      121 tgagcgcgga catcgcggcg gcgttcagga ccacggtccg gttccttatc agaacgtagg
      181 cgatcacggc gaagaggccg acgccccagc cgaggaagag gagtgactcg tacatgttcg
      241 ccgcggggat ccgctctccc gcggcccagc gcatcccgat cccccaggtc atcgcgccga
      301 agccgacgac gaggaggccg aacgaagccc agtcgagccc cttgctcgac tgcgcccagg
      361 ccgcgatcga gacgacgagc gaggcgagga ggacgagcca ggcgatgcgg gaagggttga
      421 cgcggttgta caggacctcg cgctcgacct tctccttcgt cggccaaccg gaaaggcgcg
      481 ggccgttcgc gagggcgagg agggccggcg tcgagacctc gagcgggagg tcccagcggg
      541 ccttcgggtc gctcggggcc gggatcgggc gaacgacctc gcgctggagg acggcgtaga
      601 gggcgttgag gcgttcctcg agcttctcgg cgtccttgag gacaccgaga cgcggctggt
      661 cgtgctgcgc ggctgcaccc gccgactcga tgagccgcat caccgtcggg ttcgagacga
      721 gctggtggaa ggatgcctgc ctcgccgccg ggtcgagccc cgccgcagcg gcgaggtccc
      781 gcgtgaccct cactgctggc gccgatgagg cgcctttcgg gtcgaggagc cagccgacga
      841 acgtcgcagc cgggtcctgg ccgttccagg agcgggagcc ggtcacgttc cacaccgtct
      901 ctcgcgcgag cgtgtccagc ggcatgacgc gtccgtcgtg ctgcaccggc aggcgcttca
      961 gcgcctcgac ctctgccggc gtctgcgcgc tcgtcgggcc ggccagcccc gcgagggcga
     1021 ggacggcgcc gagcgccttc cccatcgtcg gaagagacat tccgccctgc gcgaccttcg
     1081 cctcgaggtc gctccggctc ctttcgcgct cgcgagcctg cgagacgcgc gtcaggagga
     1141 cgaggatcat tccgaggacg agcgtcgcgt agccggcgaa gacgacgagc tgccccggat
     1201 ccttcgagac ggagaggacg gttgcctcgc gccctccctg ctgctggtag ctcgactgga
     1261 agagtgacca gccgcggtga tggagcgggg tattcatgaa gatccgcgtc ggaaagctct
     1321 ttcccgtgtc gaggtcggtg acgacgacct ggctcgcgaa acccgaggga cgcatggtgc
     1381 cctggtaggt ctcgaggacg aagtcgtcga gcttcaccga aaaggggagg tcgtgcttcg
     1441 cgaggaccgt ccccttctcg tcgacgtgct cgaccgtcga agacgcctgc ccttcccaga
     1501 gcccgagctg cccctccgtc ttcccgaagt aggtcagcgc cgcgccgacg aagatgagga
     1561 gaagagaagc gtgcgtcgtg acgaagccga tccgctgctt cccccacggg aagtgggtcg
     1621 cgatcgagca ggcgacgttg accgcgaaga gcccctggag ccccaggaac caccacgagt
     1681 agtagacgag ccgtcccgcg gtcaccgcat ccgtgcggga ctcgaggatc gtcccggccg
     1741 agaggccgat gaggagtacc acgaggagga cgacggcgag cttgagggat gcgagggtgc
     1801 ccacgagctt gttcaagggt tcagtccgtt tctgcgccga tcgggcggca gagccgaaag
     1861 gcagctgacg tttacttcag aggtgggggt gtcgaggagg cgggtgccgg cggtggcccg
     1921 gagcgattgt ccaagggggc ggaccgccgc cctggcctcg gaaggctgga ccgtgaggag
     1981 acctgagcgc tcgcggcgcc cgggctcctc ctgtcgcgtc attcaggtcg cttcgacagt
     2041 cggggtgtga tgccgtcctc ccttcagcgc gcgaaggccg ttcgcgacgg ccagcagctc
     2101 cgccgcttcg tgcgcgacga ccgtcatcgt gatcgtgatg gcaccggtga gcgcgaccgg
     2161 aatgaggacg gcgaggacga cgatggagaa gacgacgttc tggaggacga tcgtccgcac
     2221 cttccgcccg atcaggaacg cctcgtcgag atgctcgagg ctgtccgcca tgagcgccac
     2281 gtcggcggcc tcgatcgagg cgtcgctgcc gagcgcgccc attgcgatgc cgacctcggc
     2341 ggcggcgagg gctggggcat cgttgatccc gtcgccgacc atggcgaccg ggccggactc
     2401 ggccgaaagc gcccgcacgg catcgacctt ttcctcgggc cgaaggcccc cgcgcgcatc
     2461 gtcgactccg acggagcgcg cgacgttctc cgccacgagg ggccggtcgc cggagaggac
     2521 gacgagccgg gagacgcccg cctttcggat gtgccggatc gctgtcgaag cctccggccg
     2581 gggctcgtcg cggaacccga gaagaccgag aaaccgcccg ccagagagga cgatcgccgg
     2641 ggtcacgccg gccccggcga gctccgccag ccggtcggcg acatccccga agggaacgcc
     2701 accagcctcg ggctgaacga cctgaatgca ctcgccgtcc acgtagccct ggacgcccga
     2761 gcccgggagc gcgcggaaac gtgtcacttc ttgaaaggcg gccactccct tcgagtgcgc
     2821 gtgccggacg accgcagcgg cgagcgggtg ttcggagtga acctcgagag aagccgcctt
     2881 ccacaggagc tcccattcag tggtaccgcg tgccggttcc acgacgacga cctcgggccg
     2941 tccgcgcgtc agggtccccg tcttgtcgaa cgccacggtc cggacgcgcg cgaggacctc
     3001 gaggtaccgg ccgcccttga tcagaatgcc gtgccggccg gccgccgaga tgccggctgc
     3061 gaaggcgacc ggggtggaca tcgcgagggc acagggagcc gcggcgacga gaaggacgac
     3121 ggcgtaccgc gcccacgtgg cgaaggcgcc gccggagacg agaggcacga ggaggagaag
     3181 gacagccgcg cccaggacga gaggggagta ggcattcgtg aaacgctcga cgagctgctg
     3241 gccgcgaccc ttcgacttct gggccgcttc gacgagcttt acaaggcggc tcacggagtt
     3301 ctcggccgcc gtcgctgtgg cccggaagac gagactcccg gtccggttga gcgtgccggc
     3361 gaagagccgt tctccaggct tcttctcgat tggtatcgac tcgccggtca ggctcgattc
     3421 gtcgacggcg gattcaccct cttcgacgag tccgtccgtc gggatcgact cgccgggccg
     3481 gacccggatc cggtctccgc gctggaggag cgacgccggg acgacgacct cgcctccgtc
     3541 cctgagaacg gtggcttcgg cggggacgag gtcgaggagg cgctcgatcg cgcgcttagc
     3601 ccggtcgtac gtgaggtgct cgactgcctc ggccatgccg tagaggaaga cgagcgctgc
     3661 ggcctcgtcc cagaggccga gcgccgcggc gccgacggcg gccgcggcca tgagcacctc
     3721 gatgccgacc tcgtggcaat gcgtcagctc ctgccatccg tggcgcaccc agtccgcgcc
     3781 cgcgaggagg atcgaggcga ggtagagcgg aacgacccag gcatggccga atccgagctg
     3841 ctccagggcg aggccgccgg cgaggagaag tccgccggcg aggacgcgcc ggacggcggg
     3901 ggacctccag aatgagagtg gggcaaggga ggaggactca gccatgggcg ttgtcggatg
     3961 ggtacccgca gaggagaact tcgggcccct cctcgcccgt ccgaataccg acgacccggg
     4021 tcagagggct gacgaagatc tttccgtctc ccgggttccc tgtatgtgcc gctctccgaa
     4081 tcgtgtcaac gatcgccttc agctcagcgt cgcgacagta gatgtcgatc cgcagcttct
     4141 cctcgaacgg ctccacctcc gttcgctcgc cccagtgcgg tgcgtgcgcg tagcgacggc
     4201 cgaatccgcg gacctcactc acgctcattc ccgggaagtt cgggacgaga cggagggcat
     4261 cgacgacctg ctccagcatg aagggctgaa tgacggcgac gacaccgtgc atcgactcac
     4321 tgtccatggg ttacctccgt cttcgcggca cggccggccc ggccgaagaa gctgtagatc
     4381 gtggggagga tgaagagggt cagaatggtc gatgtcacaa ggccgccgat cacgacagtg
     4441 gccagcggcc gctggacctc ggcgcccgac ccggtcgcga gcgccatcgg cacgaagccg
     4501 agggatgcga cgagggcggt catcagtacc gggcggaggc gcagtccgca cgcctttcga
     4561 aggacgtcgg cgatgtccag gtgtgaacgt gcctcgaggt cacggatctg cgtcatcagg
     4621 acgacgccgt tcaggacggc gacgccgaag agcgcgatga acccgacggc cgcggcaatc
     4681 gagaagggaa gccctcggag ggccagtgcg atgacgccgc ccgtggcggc cagcgggacg
     4741 ttgaggtaga tgagaacggc aggcttcagc gccccgaagg tgaagtagag catcgcgaag
     4801 atcagggcga gggcgagcgg cacgacgacc atcagcctcc gggacgctct ctcgaggttc
     4861 tcgaactggc ctccccagcg gatgtagtaa ccgtcgggga gcttgacggc gctctggagc
     4921 tttgccttcg tctccgtcac gaaggacgcg acgtcgcggc cccggacgtt cacctcgacg
     4981 acgacgcggc gcttcacggc ctcgcgcgag atctgggacg gcccggtgtc caggacgatg
     5041 tcggccagct gtccgagcgg gacgagggca ccgccggggg ccgcgacggg cacgttccgg
     5101 aggccctcga tcgtctgggc cgccgagtcg tcgaaccgca ccgcaagtga gaagcggcgc
     5161 tgaccttcga acacggtccc gacgatcttc ccggcccgcg ccgcctcgac cgtgtcgagc
     5221 acgtcgccta ctgagatgcc gtaacgggcg cagcgatcgc gatcgacctt cacacggagc
     5281 accgggagac cggctgtctg ctcggccttg acgtcggccg cgccgggcac ggtggcgacg
     5341 acccgcgcga tctcctcgcc cttctgccgg aggagctcga ggtcgtctcc gaagagcttg
     5401 atgccgacgt ccgatctcac gcccgcgatg agctcgttga agcgcatctc gatcggctgc
     5461 tggaaggaga agccgacgcc gggaacggtc tcgccgaggg cctccgccat cttctcgacg
     5521 agctccccct tggatttggc cgacgtccat tcgtccttcg gcttcagcat cacgaagacg
     5581 tcccctagct cgatccccat gacgtcggtg gcgagctccg ggctacccga ccggctcacc
     5641 acagtgatca cctccgggaa cctcttcagg acccgctcga tccgccccgt gctggccgcg
     5701 acctcggaga tgccgacgga ggggggccgg acggcgctga tcgaaaggtc gccctcgtcg
     5761 aggcgaggca ggaactcgcc cccgagcgtc gaggcgacgg cggcgcccac ggcgacgagg
     5821 acgaccgcgg cgatgaccac gggggcccgc cgggagaggc agacgtcgag gccgcgaagg
     5881 tacaggaccc gcagctttcc gacgaagcga ggctcgtggt cgtgtcccgt cttcttcagg
     5941 aagagcgacg cgaggaccgg ggtgagggtc aacgtgagca ggagggagcc ggcgagggca
     6001 aagacgacgg tccaggccat cggcttgaac atctttccct cgacgccctg gaggctcagg
     6061 atgggaaggt agacgaggat gatgatgccg atgccgaagg tgatcggccg gacgacctcg
     6121 tgggcggcct cggccgtgag ctggcgcacc gtcttgtcgc gcccttctgg ctcggagagc
     6181 cgccggacga cgttctcgat gaggacgacg gccccgtcca cgatcagccc gaagtcgatg
     6241 gccccgaggc tcatgaggtt tgccgagatc cgcgactcga ccatccccgt gaaggccagg
     6301 agcatcgaga gagggatcgc ggaggccacg atgagggccg cgcgcacgtt cccgaggaac
     6361 gcgaaaagca cggcgatgac gaggatgctc ccctcgagga ggttcgtctc cacggtgcgg
     6421 atcacccttc ggacgagggc ggcccggtcg tagtagggaa cgattctgat tcccttcggg
     6481 agcgtcggct ggagctcttc cacggcctcg cgggcacgcg tggccacctg cagcgcgttc
     6541 tcaccggcga gcatctggac gaggccgatc acagtctcgc cgtggccgtc tgccgtggcg
     6601 acgccgattc gcagcatcga cccttcccgc acctgggcga tggcgccgac ggtgacgggc
     6661 gttccctcgg gggcggcctt cagcacgatc ttctcgatgt cggagagcga gctgacgagg
     6721 ccctccccgc ggacgatgta ctgctcgcgg ttgtgttcga tgtagccgcc gccggcgttc
     6781 gcgttgcctc gctcgacggc ctcgaagacc tccttcaggg agaggccatg cgcccgcagt
     6841 ttcgcgggat cgacgacgac ctggtactgc ttcggaaggc cgccccaggt gttgacttcc
     6901 gtgacgccgg gaacggcgcg cagccggggc gcgatcatcc aatcgaggat cgtccggcgc
     6961 tccatcgcgg agacgccgtc cccctcgaca gtgaactgga agacgtcgcc gagcccggtc
     7021 gtgaccggac ccagctcggg agtgcccaga ccctccggga tcgcttcgcg tgcctggacg
     7081 aggcgctcgg agacgagctg gcgggcgaag tagacgtcga ccccgtcctg gaagacgatc
     7141 gtcaccgcag agaggccata gcgggagatg gagcggatct cctggagctt cggaaggccg
     7201 ttcatcgcgg cctccaccgg gtacgtcacg aactgctcca tctccacggg cccgagggcc
     7261 ggagccttcg tgaggacctg tacctggacg ttcgtgatgt cgggctgggc gtcgatcggg
     7321 agctgcgtca gggagtaggc gccgccggcg atgaggagga ccgcggccag taggacgatc
     7381 gcccggtttt cgaggctgag gtcgacgagc cgccgcagca cctcagtcct cctccacgag
     7441 cgaggccttg gcgaactccg acttcaggac gaagctccct tcggtggcga cgacgtcgcc
     7501 agccttgatg ccggcgagaa tctcggtgaa gccctcgaag ctgtgtccga cctcgacgta
     7561 gtgccgttgg aagacgccgg gctcgacctg aacgaagaca gtcgagcgac cctccagcct
     7621 ctggatggcc gactggggaa cggccacgat cgggtgcgcc tcgctctgcg gcttcgggac
     7681 atcgacctgg gcagtgacga agagacccgg gcggagcttc tccgaacggt tctccaccgt
     7741 cgcgcggacc cggacgctcc tcgtctgcgg atcgacgacg ctgccgagga aatccacgcg
     7801 gccacggaag gtctcctgtg gatacgcgtc ggttctcagc gttacggaca gcccttcgtg
     7861 aaggagggcg aggtctttct cgtaggcatt gaggaacacc cagacggacc ccaggtcggc
     7921 aatcgtcatg agcggctgca gcgcctcgaa gagggcgccg gaggtcacct ttcggtcgat
     7981 gacgcggccg gcgaagggag cccggagcgt gagcgtggcg ccgtcagccg cggagagcct
     8041 gtggtcgaca gcgttggctc gcaaacgcgc gatcgtctct tcgctgtcac cgtagaggtg
     8101 gagcgtcctc tcggcggagg cgacggcggt cttcttcgcc aggtagtcgc cttcccgagc
     8161 ctggaactcc ccggcgctga tggcattcgc ctcaacgagc ttcctggctc tctcgtaggc
     8221 gctggccgat acgttgaagc tcgacagctc cgtcacgaac tcctcgcgcg tccgaccgag
     8281 gtcgacgctc tcgatcacga ggagcgaatc gccgggcttt acgcgagcgc ccaggtcgac
     8341 cggaatcgac gtcacccggc ccttgacgtt cgcggcgacc aggaggaggc ggttctcgtc
     8401 gaatcccacg ctgccggtga gagagagaat gtgtgagagg tcgacgatct ggaccttcca
     8461 cgtcgtgatg ccggcctcgg cgatcgcggc ggcggtgaga tggacgcgct gcggggtcgc
     8521 aggctcttcg accttctcgg cggttttcgc gggggcttca ggcgcgggag cggcaccgcg
     8581 gcccttgccg cagccggcga gagcgacggc ggcggaaagg gcgaggccgg aaaggaggat
     8641 ggtgggcttc ctgttcactt gctatctccc gtggtgtcgt cgttcgggcc gaggagccgc
     8701 cgaagctccg cgatcgcggt cgcgagctcg aagcgggcgc ggatctcctc gcggcgggcc
     8761 tcgcgggccg tgcgctgggc gtcgaggagg tcgaggaggg acgtctcgcc ctcctcgaag
     8821 aggaggcggg cgaggcggac gctctcggcg gcggatggga ggaagtctcc ctcgagcgag
     8881 gcgacctgaa cggcgagcgt ctcgacgtcc cggcggcgtg tcgcgaggtc ggagaggagc
     8941 gcgagccgca cgcgctcggc gtccgcggcc cggaggcgac tctcggcctc ggcccgcgcg
     9001 acgtctcctc gtcgggcgtt ccagaggggc agggtgaggc cgagggcgcc gccccatgcc
     9061 tcgcgatcga gctcgttctg ccggaagaag gagaagccga ggtccgggac gcgtcctctg
     9121 cgggcgctcg agaggagcga cgaggcgcgg gcggcctcgg cgcgggcgcg gatcagcgcc
     9181 gggcttcgct cgagggcgtc ggggtgcgcg tcgaggaggg cgcgcatgcc gtcggggtgc
     9241 cgtggaagct cgcccgcgag gcgaagcggg gtggggagag gctctcccgc gagtgtcctc
     9301 atgatgccct ccgcggcgag ctcctccctg tcggcggcgc tcagctgacg atcctgccgg
     9361 agccactcga cgcgcgcctt gattcgatca acctcacgcg cctcgccgag cgaggcacgg
     9421 cgggtcatca ggtcgaggag ggagcgggcg tcctccgcgg aggcccggcc cacctcgacc
     9481 tgcgcccgcg cgaacaggag acgctcgtac gtgacctgcg cgtcgagctc gaggccccaa
     9541 cgcgtcgcga ggccctcggc ccgcagcgcg cgggcctcgt ggtcggcggc ctcgatcgac
     9601 gccgatcggg ccggaagcca ggggatcgac tgcgagacgg agaagccctt ctcggtcttc
     9661 gtcgaccccg taccgtctgc cgcccgaccc tctccgaggc tcagctcgag cgtcgggtcg
     9721 ggcaagaggc gtgccgcctt cgactccgcc gaggcgctcg cgtcgagcgc cagcgacgcc
     9781 tgcgcggacg ggtgcgactg caggacgagc cggacgtagt cgtccatccg gagcccgtcg
     9841 tccgcgtgtg tttcgagggt gccgacaagc gctgccaacg cgagcacttg gcggcaatac
     9901 gatcgaattc ccattccgtc ctcctggagg cggcgggaga gaggcgcgct ccctggtcgt
     9961 gaccgagggc ctggtccgga accgactgaa tcagatccag accgcgtgcg tcgtcccgtc
    10021 gcggtggcgg cgtgccggcc ggtatggccg acccgtcagc gaagaggtcc gaggggagtg
    10081 ccgtctccgg ggcgcgtcgc tttctcgcga cgcaatccgg aaggagcctc tctcagccgc
    10141 tgctacgcga gggccggttt cgggacgtgc aggatatcgc cgacgtgcag cgtggtcgga
    10201 ggcgcggacg cctcgaacga ggagcggcac gggcgcgagg cggggctagc gagggcggga
    10261 atccccggcg agacagggct gacgcgggtg gcgcaactgc acgtggcgca cgtcggcggg
    10321 caatggccgg ggctggtgtc gtcggggcag gtctccacgc actcggcccc gagaaaaagg
    10381 gaaccgagac tcgtctcctg cactaagaag accgcgaaga acaggaagaa aatccttctc
    10441 aacaccggcg cggaagatag cacacggttg ctcaggtctc cgaaaagcgg aagccgacgg
    10501 aacccgcggt acccacttca gggatcgctt ggaaggggta tctgttttcg tcgttccaat
    10561 ctgtccctag atacccgcgg agaagtagcg gcggctgtct cgagcctggg cgaccggcac
    10621 ggctctactg gccatccact gaatcaccct cattccatag tattgacggg aagggggacc
    10681 acccttctta tcccagaggc catgaggagc ctttgtcgcg ccgatgtcac cctgcgcagc
    10741 acgaaagttg ctccacgtcc gttctgaacg acagcgcgca gagctttagg ccctcgacca
    10801 tcgtgaggta cgggaagagc tgggaggcga tgtcccctac ggtcatccct gcccgaatgg
    10861 cgagcgccgc cgcctgaatc agttcaccgg cttctgcggc gactgcttgc acgccaagga
    10921 ggcgccctgt ctccgcctcg gccacgatcc ggatgaagcc ccgggtgtcg aagttcacga
    10981 gcgcccgcgg gacgctggag agtgggaggg ttcgcgactc caccgcgaac cctgcgaatt
    11041 ccgcggcatg cacgtcgagt cctaccgtcg ccacctgcgg gtcggtgaag accacggcgg
    11101 gcacgatcga taggtcgagc ggttcgtctc cccccagcat gttcacacct gctcgcgtgc
    11161 cagccgccgc cgctacgtag acgtactccg gctgggtcgt gcagtccccg gccgcgtaga
    11221 tgtgcggcgc ggaggttcgc agatggctgt ccaccaagat cgctccgtcg ctccgcgttg
    11281 cgatcccggc ggaggggaga ttcagcgcgg aggtgttcgg ccggcgtccg gtggcgacga
    11341 ggaggcggtc gacactgatc tggtcacctc ctacctcgat cttgaagccg gcgctgtcgt
    11401 ggcgaacgga tcgcggcatc tggccctcga tgatccgcat tccttcggtc tccagtaccg
    11461 cgcctagagt ctggccgatg tcgggctcca gccgcgagag gagccgggag cgagcgacga
    11521 gcgtcacccg gctcccgagt cggaggaacg cctgggccag ctcgagcgca acgtaggatc
    11581 cgccgagcac gaggaggtgt tcagtggcgt cggaggccgc gagcgcctcg gtcgaggtcc
    11641 agtacggtgt ccccgccagt ccgtccaacg ggggcacggc gggggaggcg ccggtggcga
    11701 tgagcgcccg gtcgaaggcg aggatctcct ctgcgccatc ggcccttcgc acgcgcaggc
    11761 agtgcgcgtc ctcgaaccgg gcctcgccgc ggagcagcgt tatgtcgcgc tcagcgaggt
    11821 gggcctcgta cttggccgtc cgcagctcgt cgacccggcc ccggagctga gtcaggagcg
    11881 cgccgcggtc gatgcggggc gcagctgccg cgatcacccc gtcgaagggg ctggtcgtcc
    11941 gcacgtgggc cacctgcgcc gccctcagga ggatcttcga cggaacgcag ccgacgttca
    12001 cgcatgtgcc gccggtggtc ccgcgctcga ccatcgtcac gagcgccccg ccctgcctga
    12061 gccgcagagc cgccgcgaag gcggcggcgc cactgccgat gatcgccacc ttcagcgccg
    12121 cgcctagtgg aaccgcgcaa cagtcgcgcg cgggaacctc catgaccgga gtgggcgcga
    12181 gggtctgggg cgccttcatc gcgtcacctc accctggaga gatgaggggt agcccgcgtc
    12241 cacggtcgcg cgagtgagcg cgcccacggt cgtcttcgtg gcgtcgaacg tgacgaggac
    12301 cgtctttcgc tcgaggctca cttcagcagc ggtgacgcca tggacggcga gaagggcctt
    12361 tcggaccgtg atcgggcacg ttgggcagct catgccgggc accgagaggg tcaccgttcg
    12421 ccagctcgcc ggatctgtac gcccgctcgc tgccgcatcg gcgtgcggcg ggaggtcccg
    12481 cgtcgtcggg gccacagccg gatccgtctg cgcagggcgg atgaggtaag ggaagactgc
    12541 cgcgaggacg acgaggagcg cgacgatcgc gaagactgcc ttggaggcgc ggccggtcgc
    12601 aggcacgctg cacgccgtcc cgggcgcaca cgccggggct cggcggaaga tccggcgccc
    12661 ggcgaccagg agtgccaggc cggccccggc gaggaagagc ggtcggaagg ttgcgagtac
    12721 ctggaggttt cccacccagg ccccgccgag gccgaggctc acgaggagta gcggtccgag
    12781 acagcaggct gaggccagga gggcggcgag agcgctcgcg gtcagcgcag gaccggacgc
    12841 gggagccgta gatggggcgg tggagccggt ggcgctggtc ggtttgacgt tcatatccta
    12901 aacttagatc cgtacgtagg tacggagtca agggaatgat ggcgaccgat gcaccagcgc
    12961 gtggaatgac aatcggacgg ctggccgagg cggccggcgt ccacgtcgag accgtccgtt
    13021 actaccagcg gatcggcctg ttccccgagc ccacgcgcgc gtacggatcg gcgcgccggt
    13081 acggaccagc cgacgcggcc catcttcgct tcatcaagcg cgcccagggg ctcggcttcg
    13141 ccctcgatga ggtgcggctc ctcctccagc tcgcggtcgg cgagcactgc gccgagactc
    13201 gtatgcttgc ggagcggaaa ctcgcgctcg tcgaggagaa gctccgggac ctctccgaaa
    13261 ttcagagcgt gctccgcggt ctcgtccggg cctgcggagc gggggcgcca gggtgtggct
    13321 gcccgatcat cgatcgcctc gccggggagg ggcgttccac cctgccagcc cgccagactt
    13381 cggcgagcgg acgcgatcgg agctcatgag gcagcgacat ggcgtttgac gaactgctcg
    13441 cggatcgagt tcggagcatc ctcagtcgac gcagcgacgt cgtcgagcgg aagatgttcg
    13501 gcggcatcgc attcatggtc tccggctcga tggcctgcgg cgtcgtcgcc agcgacctga
    13561 tggtcagggt tggtgccgag cggtacaaag cggcgctggc gcgagcgcac gcacgcccca
    13621 tggacttcac gggacgaccc ctccgcggca tggtgtacgt agccgccgga ggcctggcta
    13681 ccgaatccgt tctcgaagcc tgggtcgccg aagccgtctc ggttgcgacc tccgcttcgc
    13741 cggcaccgac gctctcgcga gcgacaaaga agccccctgg tcgaaagcgg tctcgatgag
    13801 caggggtatc tattttcgct gtcccaagct gtccctagat acccgtggaa aaactcgtgc
    13861 cagcgtaaat ctatgatata ggcctagttg cggctgtccg cagacggagg tgccgaatgg
    13921 ggttcaaggg gtcgcgggtt cgaatcccgc cgtcccgacc attgattcca agtgacttag
    13981 cgccacttcc ggggcgcttt ttcgtgggcc gtcaaggcgt caggcgagga aattcctgag
    14041 ggttcgaagt cgcgacgcga cgctgggcag gtcgctgacg acgatcctcc agacgagctc
    14101 gaggtccacc gcgaagtact cgtggacgac tcggttcctg aggccgacga tccggggcca
    14161 ttccaggtcc ggttcgctcg ccttgaagtc atcgggcacg tgagtcgccg ccgccccgag
    14221 gatctcgagg ttcctgacga cggcgtcaga tgtcctttcg tcggcgagga acccgggctg
    14281 gtcgaggccc acgacatggg ctcgatccgc tccgtcgggt cgagcccgtc gtcgaggagg
    14341 aggcggacgg agcgcttgga catgtggcgc ctcgagccgg atggcgttca tgtgccgcgg
    14401 gcgaaacgcg cgggtagtga tcgggtcgac cggtccgccg agggtcggaa aggccgggcc
    14461 ggccgggtct cggttgcgac tcaggccacg gttgtccgaa cgaaggtgag ggcttgggcg
    14521 acggcgtcgg ggagggtctc gccgcgggcg aggagggcgg taaccgcgcc
//
LOCUS       DAOONL010000086        14546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4453_length_14546_cov_14.440825, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000086 DAOONL010000000
VERSION     DAOONL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4453_length_14546_cov_14.440825"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PLP50_12265"
     CDS             <1..1443
                     /locus_tag="PLP50_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52363.1"
                     /translation="PPGSAGVSFGTVPADEGFTGRATVFGFRQSVSDRTNVAVYNPGS
                     EPVSVKVTVVSAVNDRQAVLSEREDLGPYGWRQFSNPSSLAQDFDNGYVRIDRVSAGG
                     VFGAYGVVNDNDTNDGSFVPAVDEASLGTSFTIPVVVDSSAYRTELILANPGNAAATF
                     ALTFVDSISRPSVPLSATFPMPPRRQAIVPDVFELFGVGVWAGENRTRAGTLRITALE
                     EEAPGAFVGVRVLTRQAIGRFGVFAPAVPAGSADAGQGFLWGLRADEETRSNVAVLNA
                     GEPGGENVTLQLQVVDGASGFPAGAPLVITLEPGQWAQPDGFFASAGVAHGDVRIVRV
                     HGEGPWWAYGVVNDGERAGEGTGDGSFVPVVFTGVPDPLPEGEWGEPGLRIRVDGAGV
                     VIEADCAHGRIDGPVFLDTQGRFVGTGTWSFEGGPVPAGGFPEHPVRFSGQVEEEQLS
                     LTVRFLHEDVSEALRLTATRGLYPQLDKCL"
     assembly_gap    1538..1637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1638..>3492
                     /locus_tag="PLP50_12270"
     CDS             <1638..>3492
                     /locus_tag="PLP50_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA52364.1"
                     /translation="DDVLRVSANPSVPLQKLPPDPLRDSEVPDAIEWGITKTGAPSAW
                     ALGIKGAGIVVAGQDTGYDWDHPALKARYRGWNGSVASHDYNWHDAIHSGGGVCGANS
                     PVPCDDDEHGTHTMGTMVGDDGGPNQIGMAPQAKWIGCRNMNQGNGTPATYAECFQWF
                     VAPTNLAGLDPDPTKAPHVINNSWGCPPSEGCTDPLVLKTVVENTRAAGIVVVVSAGN
                     SGSSCGSVSDPPAIYEASFAVGATDSSDGIASFSSRGSVTIDGSGRLKPDVSAPGVGI
                     RSSVPGGGYASFSGTSMAGPHVAGHVALLLSARPQWKGQVGVVEDRIALSAVPRTSAQ
                     TCGGVAGTEIPNNTFGWGRIDVLASVSMVDLGIQIVDTPDPVAVGDQLTYKVYASNAG
                     LVTATGVSATVTLSPHVSFVSASSGCVHDAGVVTCDFGSVAKGTFPFRSIVVSVTGGG
                     TIQSAGSISGTLFDYDTGNNQVWVTTGAGSGSDLELAMAEAWDPALAGAPFAYTLTAK
                     NLGPAAAPGVTLIDTLPAGAAFSSASAGCAEAAGVVTCDLGTLNAGTSAVVNVTVLPG
                     AGTPSMTNAASLAGGGVDPNPGNNTAGVTTGVVAAQPASLAVDVLAGGGVS"
     assembly_gap    3493..3592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3674..4666
                     /locus_tag="PLP50_12275"
     CDS             3674..4666
                     /locus_tag="PLP50_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPA52365.1"
                     /translation="MTRTLITGITGQDGSYLAEALLAGGHEVHGLVRQSSLVQRSRLD
                     SLPACAAARVEGRLKLHYADLTDSSSLQWLVRQVQPDEVYHLAGQSHVKVSFDLPEYT
                     AETTGLGTLRLLEAVRQNVPGARFFLAATSEIFGEPAESPQNESTPVSPLNPYAAAKV
                     YALNLVRVYRRAYGLFACAGILFNHESPRRGENYVTRKIARGAARIAKGLERQLHLGT
                     LTPRRDWGWAPDTVEGMQAILGAASPGDYVLATGKTHSVGDFCDLAFRAVGLDYHAHV
                     VLDPEYTRPVDITETRGDAGKAERELGWKSRTTFEELVRRMVEAELAALEGRDA"
     gene            4719..6821
                     /locus_tag="PLP50_12280"
     CDS             4719..6821
                     /locus_tag="PLP50_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52366.1"
                     /translation="MSVVVPVYGASTDLERCLAAVLAHTDPSRHRLILVADGPLEAGP
                     RRVAEDAIATYPAVVRLLERRERQGFAAAANAGMREAEGDVVLLNSDTEVTEGWLEKL
                     GAAVRRRPRTASATPFSNNATLCSLPRPFAENALPSGHSVASFGRLVEGRSERLTPKI
                     PTGVGFCLYIAREALEEVGLLDELRFGAGYGEEVDWCLRASARGFSHVLDDATFVWHR
                     GGGSFGKEAAGRAKASERILARRYPGFLPKVARFMAEDPLAAARARVLDGLGPTRRRG
                     RSKGSVVHLVHGWPPYATGGTEVFARALAHLQAGRREVSVYARLSAPERRKGEAIELV
                     DGGVRVRLVVNDFRQRDPLSRNAIHSAALRADFDRFLDETRADVLHVHHLAGHAATLP
                     GAARRRGVPVVFQLQDWWPLCARVNFLHASGVACAGPSPLKCARCRPLTGLPPAALTN
                     PALHALRAGVMRRALRAERYLAGSRFLVRSFTEAGWLPADAPVEVLRYGLPYARPDAG
                     PREGTPRLPLRFGFVGALMPHKGAHVAVEAFRGLAPSDARLRLWGDAGADPEYAEGLL
                     RGAGAEAVTLEGKFGDGEAARVFAEMDVLLVPSVGLESYGLVVDEAMAHGVPVVASRL
                     GALPERFTEECGAFFPAGDAGALRDAVKRLVARPETVAGWRRALPRVRTLEDAGARIE
                     EIYEEVLGHAAAVGGAGR"
     gene            6917..8221
                     /locus_tag="PLP50_12285"
     CDS             6917..8221
                     /locus_tag="PLP50_12285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52367.1"
                     /translation="MSTRLEETAVPLERFAEAAGQVRALDVLASPLAPRWGGVPTQLL
                     ARRAEEERLWPTALVAPEAGRWTLRVTRQGTRLRTRLGPCGDVARSGWVGGGPGADGR
                     DAVATVVEAARLVGAEIVNVEGAAGWPLAALRALAGREPRLVVSLHDFALFCPRPNLV
                     EEPQARFCGYSTDGERCRVCLAATWRLPPGFLEAWRREATELLAAAHAIVYPSSFLLA
                     RHATLFPGPRGGLEQVIEPVAPGGGTSAPAPATRAVGREGPARVAFVGAFRPHKGARV
                     FRDLVTRREVAGRPVEWSILGSGDAALSREVRGSGVRVAGHYRAGSLARRLQEEDVDV
                     ALLLSVWPETYSLTLTECRAAGVPVLAFGHGAIAERIAAEGGGLVVPVENGAEAIAGM
                     LEKVLSGELAVPPFRGGPAGPSARGAAAARSRLYRTLLGEAT"
     gene            8245..9225
                     /locus_tag="PLP50_12290"
     CDS             8245..9225
                     /locus_tag="PLP50_12290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52368.1"
                     /translation="MRLLSVVVPTHDTRELTLRCVDAALASRLPEGTVLEIVVVDDGS
                     RDATGEALAGREGVRVVRNEEPAGFAGAANRGVSETRGEVVVLLNSDAEVEPDSLAAV
                     ASAFAADGNLGIAGAALRYPDGTPQWSAGREPDLLWLFAQASGLPALLGRVPGWRRVK
                     PLHVETPADVGWVSGAAMALRTAVWRAHGPLDASFAFYVQDLDFCFRARAGGWRVRLL
                     PAFRAMHRQGATITSNGNGTVDNLDLGLLWLDFVSWARKRGGPSFAARARTAILAGSG
                     LRLAARRAALPFLSEGRRRDWKTVTALLATARSRLLERGAGGRVDPSTAG"
     gene            9421..12282
                     /gene="uvrA"
                     /locus_tag="PLP50_12295"
     CDS             9421..12282
                     /gene="uvrA"
                     /locus_tag="PLP50_12295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPA52369.1"
                     /translation="MDSIVIRGARQHNLKNVSLTIPRNALVVLTGVSGSGKSSLAFDT
                     LFAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTVT
                     EIHDYLRLLYASIGQPHCTSCGRAISAQTIQQMTDRVLAMSEGTRFLVLAPYVRGKKG
                     EYRRQMAELAKKGFVRARVDGETVDLAEPPELDKQKKHTIEVIVDRLVVRRDDEARRR
                     LTDSLETAVRVAEGLVTISVVGGKGETLSEETLSTSYACPDCGTSLPEITPRLFSFNT
                     PYGACAACSGLGSVPRVDPDRLVPDPTLSIADGAIALWKKGSSNWRLRQLEQLSKAAK
                     FSMEVPWKKLPAGVRDMILHGSKEQEIKWKWKSEKGEYVWSSSYKGLVPLLTEKYKEV
                     ESEEARQELEAFMSLTPCSECGGRRLKAEALAVKVRGQAIDALAEMTLEDARAFFTTF
                     RPEPREREIAGKILKEIEDRLGFLNDVGIGYLSLGRASATLSGGEAQRIRLATQIGSK
                     LMGVLYVLDEPSIGLHPKDNRKLIETLMAMRDLGNTVVVVEHDEETIRAADRVVDLGP
                     GAGIHGGEVVGEGTAGELARFPRSLTGKYLSRELTIPVPAERRAGNGKAITVVGARAN
                     NLREVTARFPLGCLTAVTGVSGSGKSTLVNDILFRAAARMLHGAHEKPGEHERIEGLE
                     HVDKVIDIDQSPIGRTPRSNPATYTNLFGPIRDLMAQVPDARMRGYGPGRFSFNVKGG
                     RCEACGGGGQTAIEMHFLPDVYVTCDVCGGRRYNRETLEVRYKGLNVAEVLDLTAEQA
                     AEVFANVPTIRNVAATLVEVGLGYIKLGQPATTLSGGEAQRVKLARELARRATGRTLY
                     ILDEPTTGLHFDDVRKLLDVLHALVDRGNTVIVIEHNLDVVKTADRIVDLGPEGGARG
                     GRIVAAGTPEEVAANRSSATGAWLARTLGSGLAMRHSRR"
     gene            12292..13311
                     /locus_tag="PLP50_12300"
     CDS             12292..13311
                     /locus_tag="PLP50_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA52370.1"
                     /translation="MSRPLRLEFAGAVWHVMARGNDRKAIYADDRDREAFLEVLGRVV
                     AATRWRVHAFVLMPNHYHLLVETPEPNLSRGMRQLNGVYTQRVNVRHGRTGHVFQGRF
                     KGVLVERDAHLLELCRYLVLNPVRAGLVSGPGDWVFSSYRATAGLAPPPAWLETAWTL
                     DQLESARGSRTQAFRAFVAAGVGSGYSPWEEVEDQLYLGGAAFRARLQAIRPDLARAP
                     EISRAQRALGRPTLEAVVKEVCAASELSPGALLRRNASAGRMQVAWLGRNEAGLPLGE
                     IAPCLGATVWTVSRLESRAETLRLSDAGFRRTLEALRARLRAPRAASTDSVARRATRK
                     SQYKT"
     gene            13344..14177
                     /locus_tag="PLP50_12305"
     CDS             13344..14177
                     /locus_tag="PLP50_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52371.1"
                     /translation="MITGFNTDIKHGDRVFHVQTEDRGAGNPIVESLVYVGGQILLSK
                     RSPYADLITDGKVDEPAVRQLMDLQHRRIIEAIRRGRFDGAVVGAPETAPVPGGAALS
                     PAAAAAAAAILAGGPASGAIPVASAARPPASAALPKPAAAQPPRSGVISTNAPGAERS
                     LDQVIIEYLAAEAASERLELNLVKGGELVSGEPVALTVRASTSLTDRPVAGAQVTLRI
                     VSSIAPTQVLYRGASGADGLVKVTAALPDIGSGNAALIISATSALGTQEIKQLIRRKA
                     G"
     gene            14272..14347
                     /locus_tag="PLP50_12310"
     tRNA            14272..14347
                     /locus_tag="PLP50_12310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14305..14307,aa:Lys,seq:ctt)
BASE COUNT         1960 a         4971 c         5400 g         2015 t
ORIGIN      
        1 ccccccggga gcgcgggggt ctccttcggc accgttcccg ccgacgaggg gttcaccggt
       61 cgcgccaccg tcttcgggtt ccggcagagc gtctccgacc ggacgaacgt cgccgtctac
      121 aacccgggct ccgagccggt ctcggtgaag gtcacggtgg tctccgccgt gaacgacagg
      181 caggccgtcc tttccgagag ggaagacctg ggaccgtacg gctggaggca gttctcgaat
      241 ccgtccagcc tggcccagga cttcgacaac gggtacgtcc ggatcgacag ggtctcggcg
      301 ggaggcgtct tcggggccta cggcgtcgtc aacgacaacg acacgaacga cggctccttc
      361 gttccggccg tcgacgaggc gagcctcggg acgtccttca cgatccccgt cgtcgtcgac
      421 tcttccgcgt accggacgga gctgattctg gcgaaccccg ggaacgcggc ggcgacgttc
      481 gccctgacgt tcgtcgactc gatctcccgt ccgagcgtgc ccctctccgc gaccttcccg
      541 atgccgcccc gccgccaggc catcgttccc gatgtcttcg agctgttcgg cgtcggggtc
      601 tgggcgggtg agaaccggac gcgggccggg accctccgga tcacggctct cgaggaggag
      661 gcgcccgggg cgttcgtcgg cgtccgcgtc ctcactcggc aggcgatcgg tcgtttcggc
      721 gtcttcgccc cggccgtccc ggcgggttcc gcagacgccg gccagggctt cctctggggc
      781 cttcgcgcgg acgaggagac ccggtccaac gtcgccgtcc tgaacgcggg cgagccggga
      841 ggcgaaaacg tcaccctcca gctccaggtc gtcgacggtg ccagcgggtt ccctgcgggc
      901 gcgccccttg tgatcaccct cgagccgggg cagtgggccc agcccgacgg gttcttcgcg
      961 agcgccggtg tcgcccacgg cgacgtccgg atcgtccgcg tccatggcga gggcccctgg
     1021 tgggcctacg gcgtcgtgaa cgacggcgaa cgggctgggg aggggacggg agacggaagc
     1081 ttcgtcccgg tcgtcttcac gggagtcccg gatcccctgc ccgaaggcga gtggggggag
     1141 ccaggtctcc ggatccgggt ggacggagcc ggggtcgtca tcgaggccga ctgcgcccac
     1201 gggcggatcg acgggcccgt cttcctcgac acgcagggac gcttcgtcgg gacggggacc
     1261 tggtcctttg agggaggccc ggtgcccgcc ggggggttcc ccgagcaccc ggtccgcttc
     1321 tccggacagg tcgaggagga gcagctcagc ctcaccgtcc gtttccttca cgaggacgtt
     1381 tccgaagccc tccggctgac cgccacacga ggcctctacc cgcagctgga caagtgcctg
     1441 taacgagaat ctgaggcgcc cggcccgccg gcgaggcccc cgggtctcga ggcgggccat
     1501 ggccggcgcc ggcggctata ctcgcccggt tcagcagnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnggg acgacgtcct gcgcgtctcg gcgaacccga gcgtccccct
     1681 ccagaagctc ccgcccgatc cgctccgcga ttccgaggtg ccggacgcga tcgagtgggg
     1741 gatcacgaag acgggcgcgc cgtccgcgtg ggccctcggc atcaaggggg cagggatcgt
     1801 cgtcgccggt caggacaccg ggtacgactg ggatcatccg gcgctgaagg ccaggtacag
     1861 gggctggaac ggctctgtcg ccagccacga ctacaactgg cacgacgcca tccactcggg
     1921 gggcggcgtc tgcggcgcca attcccccgt cccgtgcgac gacgacgagc acggcaccca
     1981 caccatgggg acgatggtcg gggacgacgg aggcccgaac cagatcggca tggcccccca
     2041 ggcgaagtgg atcggctgcc ggaacatgaa ccaggggaac ggcactccgg cgacgtacgc
     2101 cgagtgcttc cagtggttcg tcgcgccgac gaacctcgcc ggtctcgacc ccgacccgac
     2161 gaaggcgccg cacgtcatca acaactcctg gggctgcccg ccgagcgagg ggtgcactga
     2221 cccgctcgtc ctgaagaccg tcgtcgagaa cacccgcgcc gcggggatcg tcgtcgtcgt
     2281 ctccgccggc aacagcggct cctcctgcgg ctccgtgagc gaccccccgg cgatctacga
     2341 ggcctccttc gccgtgggcg cgacggactc ctccgacggc atcgcgtcgt tctcgagccg
     2401 cggatccgtg acgatcgacg gctccgggcg gctcaagccg gacgtctcgg cgccgggggt
     2461 gggcattcgc tcgagcgttc ccggcggcgg gtacgcgtcg ttcagcggaa cgtcgatggc
     2521 cggcccgcac gtcgcgggtc acgtcgcgct tctcctctcg gcgaggcccc agtggaaggg
     2581 gcaggtcggc gtggtcgagg accggatcgc cctttccgcc gtcccgcgca cgagcgcgca
     2641 gacctgcggg ggggtagccg ggaccgagat cccgaacaac acgttcggct gggggcgcat
     2701 cgacgtcctc gcctcggtga gcatggtcga cctcgggatc cagatcgtcg acacgcccga
     2761 cccggtggcc gtcggcgacc agctgacgta caaggtctac gccagcaacg ccggcctcgt
     2821 cacggcgacg ggcgtcagcg ccaccgtgac cctctctccc cacgtctcct tcgtttcggc
     2881 ctcgtcgggc tgcgtgcacg acgccggcgt cgtcacctgt gacttcggtt cggtcgccaa
     2941 ggggaccttc cccttcagaa gcatcgtcgt gagcgtcacc ggaggcggga cgatccagag
     3001 cgcgggcagc atctcgggca cgctgttcga ctacgacacc ggcaacaacc aggtgtgggt
     3061 tacgacgggc gcgggaagcg gctcggacct cgagctcgcg atggccgaag cgtgggaccc
     3121 cgcgctcgcc ggcgcgccgt tcgcctacac cctgaccgcg aagaacctcg ggcccgctgc
     3181 ggcgccgggc gtgactctca tcgacacgct cccggcagga gcggccttct cgtcggcctc
     3241 cgcgggctgc gcggaggcag cgggcgtcgt cacctgcgac ctcggcacgc tgaacgccgg
     3301 cacctcggcc gtcgtgaacg tcacggtcct cccgggtgcc ggcacgccgt cgatgacgaa
     3361 cgcggccagc ctcgccggtg gaggcgtcga cccgaacccc gggaacaaca ccgccggcgt
     3421 gacgaccggc gtcgtcgcgg cgcagccggc gagcctcgcg gtggacgtcc tcgccggagg
     3481 cggcgtttcc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcgggct
     3601 ggagctgcat tgacgaacgg gacgtcgatg accggcacgc cctgagcttc cgggagacgc
     3661 agggacggaa ctcatgactc gaacgctgat caccgggatc acgggccagg acggctccta
     3721 tctcgcggag gccctcctcg ccggcggcca cgaggtccac ggcctggtga ggcagtcgag
     3781 cctcgtgcag cggagccgcc tggactccct cccggcctgc gcggccgcgc gggtcgaggg
     3841 acggctgaag ctccattacg ccgacctgac cgactcctcg agcctccagt ggctcgtgag
     3901 gcaggtccag ccggacgagg tctaccacct ggcggggcag agccacgtga aggtgtcgtt
     3961 cgacctgccg gaatacacgg ccgagacgac ggggctcggt acgctgcgcc tcctcgaggc
     4021 cgtgcggcag aacgtccccg gggcgcggtt cttcctggcg gccacgagcg agatcttcgg
     4081 cgagccggcg gagagtccgc agaacgagtc gacgccggtg tcgccgctca acccgtacgc
     4141 cgcggcgaag gtctacgccc tgaacctcgt tcgggtctac cggcgggcct atggtctctt
     4201 cgcttgcgcc gggatcctct tcaaccacga gagcccgcga aggggcgaga actacgtgac
     4261 gcgcaagatc gcgcgcggcg cggcccgaat cgccaaggga ctggaaaggc agctgcacct
     4321 cggcacgctg acgccccgac gggactgggg ctgggcgccc gacaccgtcg aaggaatgca
     4381 ggcgatcctc ggcgccgcgt cgccggggga ctacgtcctg gcgacaggga agacgcactc
     4441 ggtgggcgac ttctgcgacc tcgcgttccg ggcggtgggg ctcgactacc acgcccacgt
     4501 cgtcctcgat ccggagtaca cgcggccggt ggacatcacc gagacccgcg gcgacgccgg
     4561 gaaggccgag cgcgagctgg ggtggaagtc acggacgacg ttcgaggagc tggtccgccg
     4621 aatggtggag gcggaactgg ccgcgctcga gggccgggac gcctgacggg gtgagggagc
     4681 cggccgggaa cgggcgggga acgggagcgg cgggcgcggt gagcgtcgtc gtccccgtct
     4741 acggcgcctc gaccgatctg gagcgttgcc tcgcggcggt cctcgcgcac acagacccgt
     4801 cgcgccaccg gttgatcctg gtggcggacg gccccctcga ggcggggccg aggcgagtgg
     4861 ccgaggacgc gatcgcgacc tacccggcgg tggtgcgcct cctcgagcgg cgcgagcggc
     4921 aggggttcgc ggccgcggcg aacgcgggga tgagggaggc ggagggagac gtcgtcctcc
     4981 tcaacagcga caccgaggtg acggagggat ggctcgagaa gctcggggct gccgttcgcc
     5041 gccgcccgcg gaccgcctcc gcgacaccct tctcgaacaa cgccacgctc tgctcgctgc
     5101 cccggccctt cgccgagaac gcgctgccgt cggggcactc ggtggcctct ttcgggcgcc
     5161 tcgtcgaggg gcgctcggag cgcctcacgc cgaagatccc caccggggtc gggttctgcc
     5221 tctacatcgc ccgcgaagcc ctcgaggaag tcggccttct cgacgagctc cgcttcggcg
     5281 ccggctacgg agaggaggtg gactggtgcc tgcgcgcgtc ggcccggggc ttctcgcacg
     5341 tcctcgacga cgcgacgttc gtctggcacc gggggggtgg gagcttcggg aaggaggcgg
     5401 ccgggcgggc gaaggcgtcc gagcggattc tggcccgccg gtacccgggg ttcctgccga
     5461 aggtcgcacg tttcatggcc gaggacccgc tcgccgcggc gcgagcgagg gtgttggacg
     5521 ggctcggtcc gacgcggcga cggggtcgtt cgaagggaag cgtcgtccac ctcgtccacg
     5581 ggtggccgcc gtacgcgacg ggcgggacgg aggtcttcgc gcgggcgttg gcgcacctgc
     5641 aggcagggcg gcgggaggtg tcggtgtacg cccggctctc ggcgccggag cggcggaagg
     5701 gggaggcgat cgagctcgtg gacggcggcg tccgcgtgcg gctcgtcgtg aacgacttcc
     5761 ggcagaggga tccgctctcg cgaaacgcga ttcacagcgc cgcgctccgc gcggacttcg
     5821 accggttcct cgacgagacg cgggccgacg tcctccacgt tcaccacctc gcagggcacg
     5881 ccgcgacgct gccgggggcc gcgagacgtc gcggcgtgcc ggtcgtcttc cagctccagg
     5941 actggtggcc gctctgcgcc cgggtgaact tcctccacgc cagcggcgtg gcctgcgcgg
     6001 ggccgtcccc gctgaagtgc gcccgctgcc ggccgctcac cggtctccct ccggcggctc
     6061 tgacgaaccc ggcgctccat gcgctgcgcg cgggcgtcat gcggcgcgcg ctgcgggcag
     6121 agcgatacct ggccggctct cgcttcctcg tacggagctt cacggaggcg ggctggctgc
     6181 cggcggacgc gccggtcgag gttctgcgct acggcctccc gtacgcccgg cccgacgccg
     6241 ggccgcgcga ggggacgccg cggctgccgc tgcgattcgg gttcgtcggg gcgctgatgc
     6301 cgcacaaggg ggcacacgtg gccgtggagg cctttcgcgg gttggcgcct tccgacgcgc
     6361 gactccgact ctggggagac gcgggagccg acccggagta cgccgaaggg ctccttcgag
     6421 gggcgggcgc ggaggcggtg accctggagg ggaagttcgg ggacggagag gcggcgaggg
     6481 tcttcgcgga gatggacgtc ctcctcgtgc cgagcgtcgg cctcgagtcg tacggactcg
     6541 tcgtcgacga ggcgatggcg cacggagtgc cggtcgtggc gagccgcctc ggcgccttgc
     6601 ccgagaggtt cacggaggag tgcggggcgt tctttccggc gggagacgcg ggcgcgttga
     6661 gggacgccgt gaagcggctc gtcgcgaggc cggagacggt ggccgggtgg cgccgggcgc
     6721 tcccgcgggt gcggacgctg gaggacgcgg gcgcgaggat cgaggagatc tacgaggagg
     6781 tcctcgggca cgcggcggcc gtcgggggcg cgggccggtg aagggcgcct cccgccgcct
     6841 gcggctggcc ttcgagctcc ttcgcgcgga aggtccacgc gggcatgcgg agaggctcgc
     6901 cgagagggtg gagacgttga gcacgaggct cgaggagaca gcggttcctc ttgaacgctt
     6961 cgccgaggct gcgggccagg tgcgggcgct cgacgtcctc gcgtcgccgc tcgccccgcg
     7021 ctggggcggg gtcccgaccc agctcctggc gcggcgcgcc gaggaggagc gcctttggcc
     7081 gacggcgctc gtcgcgcccg aggcgggccg ctggacgctg cgggtgacgc ggcaggggac
     7141 gcgtctgcgg acgcggctgg ggccgtgcgg ggacgtcgcg agaagcggat gggtgggcgg
     7201 cgggccggga gcggacggcc gcgacgcggt ggcgacggtc gtcgaggcgg cgcggctcgt
     7261 cggggcggag atcgtgaacg tcgagggggc cgccgggtgg cctctcgcgg ctctgcgggc
     7321 cctcgcgggg cgcgagccgc gcctcgtcgt ctcgctccac gacttcgccc tcttctgccc
     7381 gcggccgaac ctcgtcgagg aaccgcaggc gcgcttctgc ggttactcga cggacgggga
     7441 acggtgccgc gtctgcctcg cggcgacgtg gcggctcccg ccgggattcc tcgaggcctg
     7501 gaggcgggag gcgacggagc ttctcgcggc ggcccacgcg atcgtctacc cgtcgagctt
     7561 cctgctggcg cggcacgcga cgctcttccc gggtcctcgt ggggggctcg agcaggtcat
     7621 cgagcccgtc gccccgggcg ggggcacgag cgctccggcc cctgccaccc gtgccgtcgg
     7681 gcgcgagggg ccggcccgcg tcgctttcgt cggggccttc cggccgcaca agggcgcccg
     7741 agtcttccgc gacctcgtga cgaggcgcga ggtcgcgggg cggccggtcg agtggtcgat
     7801 cctcgggagc ggcgacgcgg cgctctcgag ggaggtccgg ggcagcggcg tgcgcgtcgc
     7861 ggggcactac cgggcgggga gcctcgcacg gcggctccag gaagaggacg tcgacgtggc
     7921 gctccttctc tccgtctggc ccgagacgta ctctctgacc ctgaccgagt gccgtgctgc
     7981 cggcgtcccg gtcctggcgt tcggccacgg ggcgatcgcc gagcggatcg cggcagaagg
     8041 gggcggcctc gtcgttccgg tggagaatgg ggccgaggcg atcgcaggga tgctggagaa
     8101 ggtcctttcg ggagagctcg cggtgccgcc gtttcgcggt gggcccgcgg ggccgtcggc
     8161 ccgcggcgcc gcggccgcgc ggtcgaggct ctaccggacg ctcctcgggg aggcgacctg
     8221 agagccgccg gtccgccctc cgcagtgcgg ctcctctcgg tcgtcgtccc gacgcacgac
     8281 acgagggagc tgacactgcg ctgcgtggac gccgccctgg cttcgcggct gccggagggc
     8341 acggtgctcg agatcgtcgt cgtcgacgac ggaagccgcg acgccacggg ggaggcgttg
     8401 gcggggcggg agggggtccg cgtcgtccga aacgaggagc cggccgggtt cgccggcgcc
     8461 gcgaaccgcg gcgtctcgga gacgagaggg gaggtcgtcg tcctcctcaa cagcgatgcc
     8521 gaagtcgagc cggatagcct cgccgcggtg gcgtcggcct tcgcggccga cgggaacctc
     8581 ggcatcgccg gcgccgcgct gaggtatccc gacggcacgc ctcagtggag cgcggggcgc
     8641 gagccggacc tgctctggct cttcgcgcag gccagcggcc tgccggcgct tctcggacgg
     8701 gttcccgggt ggcgccgggt gaagccgctg cacgtggaga cgccggcgga cgtggggtgg
     8761 gtgtcggggg ccgcgatggc gctgcgtacc gccgtctggc gcgctcacgg accgctcgac
     8821 gcctccttcg cgttctacgt tcaggacctc gacttctgct ttcgcgctcg tgcgggcggc
     8881 tggcgagtcc gcctccttcc cgcctttcgc gcgatgcacc ggcaaggcgc gacgatcacg
     8941 tcgaacggca acggcacggt cgacaacctc gacctcggcc tcctctggct cgacttcgtc
     9001 tcctgggccc gcaagagggg cgggccgtcc ttcgcggcgc gggcacgcac ggcgatcctc
     9061 gcgggctcgg ggcttcgcct ggccgcgagg cgggccgccc tcccgttcct ttccgaggga
     9121 cgccggcgtg actggaagac cgtgaccgcc ctcctggcga cggcgcggag ccgcctcctc
     9181 gagaggggag ccggcggtcg ggtcgacccg tcgacggccg gctgacgccg gctgctccgt
     9241 ccccggcccc gggcccggcg tgcctcatcg cgtcccgaag gggggctcgg gtacgccttc
     9301 gacgtgcttc ggtcgtccgt agagtgtaga aagtagacct ggcccctacc tccgtgactc
     9361 catcctcggt ccccgggggg gataatccgc cccttccgac cgtcggaaac gaaagggagc
     9421 atggattcga tcgtcatccg cggtgcgcgg cagcacaacc tcaagaacgt ctcgctgacc
     9481 atcccgcgga acgcgctcgt cgtcctgacg ggggtgtcgg ggtcggggaa gtcgtccctc
     9541 gcgttcgaca cgctcttcgc ggaggggcag cggcggtacg tggagtccct ctcggcgtac
     9601 gcgcggcagt tcctcgagca gatggagaag cccgacgtcg actcgatcga ggggctctcg
     9661 cccgcgatct cgatcgagca gaagacgacg tcgaagaacc cgcggtcgac cgtcgggacc
     9721 gtgaccgaga tccacgacta cctccggctc ctctacgcgt cgatcggaca gccgcactgc
     9781 acgagctgcg gccgcgcgat ctcggcgcag acgatccagc agatgaccga ccgggtcctc
     9841 gcgatgagcg aagggacgcg gttcctcgtc ctcgccccgt acgtccgcgg gaagaagggg
     9901 gagtaccggc gtcagatggc cgagctcgcg aagaaggggt tcgtgcgggc gcgcgtcgac
     9961 ggggagacgg tcgacctggc cgagccgccg gagctcgaca agcagaagaa gcacacgatc
    10021 gaggtcatcg tcgaccgcct cgtggtccgg cgcgacgacg aggcgcgtcg ccggctgacc
    10081 gactcgctcg agacggcggt gcgcgtggcg gaaggcctcg tgacgatctc ggtcgtgggc
    10141 ggcaaggggg agacgctctc ggaggagacg ctctcgacgt cgtacgcctg ccccgactgc
    10201 gggacgtcgc tcccggagat cacgccgcgc ctcttctcgt tcaacacgcc gtacggggcg
    10261 tgcgcggcct gctcgggcct cgggtcggtg ccgcgcgtcg accccgaccg cctcgtcccc
    10321 gacccgacgc tctcgatcgc cgacggcgcg atcgcgctct ggaagaaggg gtcgtcgaac
    10381 tggcgcctcc ggcagctcga gcagctctcg aaggcggcga agttctcgat ggaggtcccc
    10441 tggaagaagc tcccggccgg cgtccgcgac atgatcctcc acggctcgaa ggagcaggag
    10501 atcaagtgga agtggaagtc ggagaagggg gagtacgtct ggtcgtcctc ctacaagggg
    10561 ctcgtcccgc tcctgacgga gaagtacaag gaggtcgaga gcgaggaggc gcggcaggag
    10621 ctcgaggcgt tcatgtcgct cacgccgtgc tcggagtgcg gcgggcggcg gctgaaggcc
    10681 gaggcgctcg cggtgaaggt gcgcgggcag gcgatcgacg cgctcgcgga gatgacgctc
    10741 gaggacgcga gggccttctt cacgacgttc cggccggagc cgcgggagcg tgagatcgcc
    10801 gggaagatcc tgaaggagat cgaggaccgc ctcggcttcc tgaacgacgt cgggatcggc
    10861 tacctctcgc tcggccgcgc gtcggcgacc ctctcggggg gcgaggcgca gcggatccgg
    10921 ctcgcgacgc agatcggctc gaagctgatg ggcgtgctct acgtcctcga cgagccgtcg
    10981 atcggcctcc acccgaagga caaccggaag ctgatcgaga cgctgatggc gatgcgcgac
    11041 ctcgggaaca ccgtggtcgt cgtggagcac gacgaggaga cgatccgcgc cgcggaccgg
    11101 gtcgtggacc tcggcccggg agcgggcatc cacggcggcg aggtcgtggg cgaggggacg
    11161 gctggcgagc tggcccgctt cccccggtcg ctgacgggga agtacctctc gcgcgagctg
    11221 acgatccctg tgcccgcgga gcgccgcgcc gggaacggga aggcgatcac ggtcgtcggc
    11281 gcgcgggcga acaacctgcg cgaggtgacg gcgcgcttcc cgctcggctg cctgacggcg
    11341 gtgacgggcg tctcggggtc ggggaagtcg acgctcgtga acgacatcct cttccgggcg
    11401 gcggcccgga tgctccacgg cgcgcacgag aagccggggg agcacgagcg gatcgaggga
    11461 ctcgagcacg tcgacaaggt gatcgacatc gaccagtcgc cgatcgggcg gacgccccgc
    11521 tcgaacccgg cgacgtacac gaacctcttc gggccgatcc gcgacctgat ggcccaggtg
    11581 cccgacgcga ggatgcgggg ctacgggccg gggcgcttct cgttcaacgt gaagggggga
    11641 cgctgcgagg cgtgcggcgg cggcgggcag accgccatcg agatgcactt cctccccgac
    11701 gtctacgtga cgtgcgacgt ctgcggcggc cgccgctaca accgcgagac gctcgaggtg
    11761 cgctacaagg ggctgaacgt ggccgaggtg ctcgacctga cggccgagca ggccgccgag
    11821 gtcttcgcga acgtgccgac gatccggaac gtcgccgcga cgctcgtgga ggtcgggctc
    11881 ggctacatca agctgggcca gccggcgacg acgctctcgg ggggcgaggc gcagcgggtg
    11941 aagctcgccc gcgagctcgc gcggcgcgcc acggggcgaa cgctctacat cctcgacgag
    12001 ccgacgaccg gcctccactt cgacgacgtg cggaagctcc tcgacgtcct ccacgccctc
    12061 gtcgaccgcg gcaacacggt gatcgtcatc gagcacaacc tcgacgtcgt gaagacggcc
    12121 gaccggatcg tcgacctggg gccagaggga ggcgcccgcg gagggaggat cgtcgccgcg
    12181 gggaccccgg aggaggtggc cgccaaccgc tcctccgcca ccggcgcctg gctcgcccgg
    12241 acgctggggt caggtcttgc gatgaggcat tcgcgccgat aggatcgcct catgtcgagg
    12301 cccttgcggc tcgagttcgc cggcgcggtc tggcacgtga tggcgcgcgg gaacgaccgg
    12361 aaggcgatct acgcggacga tcgcgaccgc gaggcgttcc tggaggtgct cggacgggtc
    12421 gtggcggcga cgcgctggcg ggtgcacgcg ttcgtcctga tgccgaacca ctatcacctc
    12481 ctcgtggaga cccccgagcc gaacctctcg cgcgggatgc ggcagctgaa cggcgtctac
    12541 acgcagcggg tcaacgtgcg gcacggccgg acgggacacg tcttccaggg gaggttcaag
    12601 ggggtcctcg tggagcgcga cgcccacctc ctcgagctct gccggtacct cgtcctgaat
    12661 ccggtgcggg ccgggctcgt ctccggcccg ggggactggg tcttcagcag ctaccgggcg
    12721 acggctggtc tcgccccgcc gccggcatgg ctggagacgg cctggaccct cgaccagctc
    12781 gagtcggccc gcggctcgcg aacgcaggcc tttcgcgcgt tcgtagcggc tggcgtcggc
    12841 tcggggtact ccccgtggga ggaggtggag gaccagctct acctcggagg agccgcgttc
    12901 cgggcccggc tccaggcgat ccggcccgac ctcgcccggg ctccggagat ctcccgggcg
    12961 cagagagccc tcggccggcc gacgctcgag gccgtcgtga aggaggtctg cgccgcctcc
    13021 gagctcagcc cgggcgcgct cctccggcgg aacgcctccg cgggacggat gcaggtcgcc
    13081 tggctcggcc ggaacgaggc cggcctcccg ctcggggaga tcgctccgtg cctgggcgcg
    13141 acggtctgga cggtttcccg cttggagtcg cgagcggaga ccctccggct ttcggacgcc
    13201 ggatttcgac ggacgctcga ggcgctccgc gcccgcttgc gcgcgccacg cgccgcgtcg
    13261 acggactcgg tcgctcgccg ggcgacgcga aagtcacaat acaagacctg accccaggct
    13321 cgggctagaa tccagggtct gtcgtgatta cgggcttcaa caccgacatc aagcatggag
    13381 accgggtctt ccacgtccag acggaggacc ggggggccgg gaacccgatc gtggagtcgc
    13441 tcgtctacgt cggggggcag atcctgctgt cgaagaggag cccctacgcc gacctgatca
    13501 cggacggaaa agtcgacgag cccgcggtcc gccagctgat ggacctccag cacaggagga
    13561 tcatcgaggc gattcggcgg ggtcgcttcg acggggccgt ggtgggagcc ccggagaccg
    13621 cgccggtccc gggcggcgcc gcgctctccc cggccgccgc ggccgcggcg gccgcgatcc
    13681 tggccggtgg tcccgcctcg ggcgcgatcc cggtcgcgtc ggcggcccga ccgcccgcat
    13741 cggcggcgct cccgaagccc gcggccgcgc agcctccgag gtcgggggtc atctcgacga
    13801 acgcgccggg ggcggagcgg tcgctcgacc aggtcatcat cgagtacctc gcggccgagg
    13861 cggcgtcgga gcgcctcgag ctgaacctcg tgaagggggg cgagctcgtc tcgggcgagc
    13921 cggtggcgct cacggtgagg gcctcgacgt ccctcaccga ccggcccgtc gccggagcgc
    13981 aggtgacgct ccggatcgtc tcgagcatcg ccccgacgca ggtcctctac cgcggcgcga
    14041 gcggcgccga cggcctcgtg aaggtgacgg cggccctccc cgacatcggg agcgggaacg
    14101 cggccctgat catctcggcc acctcggcgc tcgggacgca ggagatcaag cagctgatcc
    14161 gccggaaggc gggctgaccc tttccggggc gccggcgtcg ccgcacttga acttccgaac
    14221 gctctccgta taatcccctt cccgcgtccg ggtcggccgg ccgcacgcca cgggccgtta
    14281 gctcaattgg tagagcaaga gactcttaat ctctgggttc ggggttcgag tccctgacgg
    14341 ctcaccagca ccttccgggg gtcggcggag acgccggccc ccgattcgta ttcgggggcg
    14401 ccgcgagggc tcacggcccc ttccggtggc gagaggcccc tgtggtaacg tcgccgtccc
    14461 cgtccgctcc gcgggagtgg cggaatggca tacgcgccgg acttaggatc cggtggggcg
    14521 acccgtgagg gttcgagtcc ctcctc
//
LOCUS       DAOONL010000087        13531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_4989_length_13531_cov_15.865910, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000087 DAOONL010000000
VERSION     DAOONL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_13531_cov_15.865910"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="sufB"
                     /locus_tag="PLP50_12315"
     CDS             complement(<1..842)
                     /gene="sufB"
                     /locus_tag="PLP50_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPA52372.1"
                     /translation="MSSNEVLSELTSQEYRHGFVTEIESDVFPPGLTEEIVAAISAKK
                     KEPQWLLDWRLKAFRHWQTMTEPRWAKVSYPAIDYQAIRYYAAPKSTKDGPKTLDEVD
                     PELLRTYEKLGVPLEERAILAGVAVDAVFDSVSVATTFRESLAKVGVIFCSFSEAVQE
                     HPELVKKYLGSVVPYTDNFFATLNSAVFSDGSFCFVPKGVRCPMELSTYFRINQPDTG
                     QFERTLIVAEEGASVSYLEGCTAPKRDTNQLHAAVVELVALDDAKIKYSTVQNWYPGD
                     KDGKG"
     gene            complement(839..1300)
                     /locus_tag="PLP50_12320"
     CDS             complement(839..1300)
                     /locus_tag="PLP50_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HPA52373.1"
                     /translation="MNKLTDYGVVLLTTFAHEPALGISARELSVRTEIPQPTVVKLLK
                     TLLKAGLLVSQRGTKGGYALARSPEEIPVAAIIEALEGPLAITECSLPGTCAHERGCP
                     ARPNWKAVNDVIQDALSRLTLADMTRPASSGATRTGSPLPAVDSAPRSIAS"
     assembly_gap    1580..1679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1680..3003)
                     /locus_tag="PLP50_12325"
     CDS             complement(<1680..3003)
                     /locus_tag="PLP50_12325"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPA52374.1"
                     /translation="MTPLLADLASWWLQSGLLLGAALLVPAAVRLRDPAARLRIAQAA
                     LLAAFALPFVQPPRPADGAGGASIRTAFSLVVPVEPGATAGAPSLETAVLAALSAGAL
                     LRLGWLGAGLLSLRALRRRARPLAPVPASVVSAEERTGACAELLVSSEVASPVAIGWR
                     RPAVILPERFAALGASEQEAVACHELLHVKRRDSRAVLLEELARALLWSQPAAWGLLS
                     GISLAREQAIDGDVVRSTGDRRSYLRALARLAAFAQEAPSGALPFHTRSHLLRRVAHL
                     AKEVPMSRRSLVLATTAAAALLLLVGAAGAAVVPFGSSAGDPGAEPAASSAASPSDAV
                     REGVAGGVWGGAAGGVAEGTGDDVILKVGGDVKEPVEIQRVQPTYPEEARKNRVQGRV
                     VVRAVIDEKGVVTKVEAVESSDPMLTESALDAVKKWTYKPATKKGKPVK"
     gene            complement(3000..3377)
                     /locus_tag="PLP50_12330"
     CDS             complement(3000..3377)
                     /locus_tag="PLP50_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPA52375.1"
                     /translation="MRPVPTPLTEAELEIMHVVWELGDATVRQVHDVLSARRPVAYTT
                     VMTMLGLLAKKGHLKREESGRAFVYRPAHPKGRVVSRMLDDFVERVFRGSARPLVLAL
                     LKDRKISKRELDEIARLAEEHDR"
     gene            3571..4341
                     /locus_tag="PLP50_12335"
     CDS             3571..4341
                     /locus_tag="PLP50_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52376.1"
                     /translation="MSFRVGGGTIVGLLGPNGAGKTTTVSIVCGLVPPDAGEVKVGGV
                     ALSGDTDPAKGRLGLVPQEPALYEELTAEDNLRFFGALQRMEGARLASRIDAVLRLTG
                     LLERRRDRVKVYSGGMKRRLNLAAGLLHDPEVLLLDEPTVGVDPQSRNAIFESLEALR
                     DAGKAILYTTHYMEEAERLCDRIVIVDRGRVVAEGTKDELGALLPGNGARDLDDEARA
                     AVALLAARGIAVPGADGGTPRLEKVFLHLTGHALRDEP"
     gene            4338..5636
                     /locus_tag="PLP50_12340"
     CDS             4338..5636
                     /locus_tag="PLP50_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52377.1"
                     /translation="MKQALAIVVHELKLFLSDRRSVVVGIVTPILLATFFGFLFSGAG
                     RQDAAKIPVLVADADDSAVSRAVVAGLAADALLSVSPAAAEDARQAVRRGKAAAGVVL
                     PKGFGEGAVQAFFGAAAASEIVLLVDPSRATEAGLVRGLLAEKVFREASREAFGGATG
                     TKVLEESLVALESDPSPPTAERQALKDLLRDVTRWQGAAKGGTAGGGAAGGLGTAVPF
                     TLKEEPVTARQGVTYNSYAHSFAGMAIQFLLFVAIDFGIALLLERQGGLWKRLRSAPL
                     SRSALLLGKTAAGALVGLVSVAATFAFGIVVLGIRIEGSVPGFVLLLLASALMASSFG
                     LLLAALGRTPGATRGLAILAVLLLVMLGGGWVPAFVFPAWLQKVTLVLPTRWAIDGLD
                     AVTWRGAGLAAGALPAVAVLLGYTVLFLLVARLRFRWTDR"
     gene            complement(5867..6616)
                     /locus_tag="PLP50_12345"
     CDS             complement(5867..6616)
                     /locus_tag="PLP50_12345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPA52378.1"
                     /translation="MLQPATPPDRPRPALLASIGLHLTAFSVLGFGPLLLFPDVPGWR
                     GEVWAVTQPDLSVLRETRTVDLRERPAPARSGGAPRGGGVPVAARPGTVARPLRPATQ
                     PGRIPDELPPAGPFEPDGEPGVPGGFDDGTSTTAGTGVGDEDPGGAPLYDVRGGALPP
                     DLVLPVPIDTPAPAYSEAARIVRAAGVVVLSATIASDGRVVDVAVENDAHPVLERLAI
                     EAVSSWRYTPARLGARPVSVVLRVTLTFRLR"
     gene            6736..7131
                     /locus_tag="PLP50_12350"
     CDS             6736..7131
                     /locus_tag="PLP50_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HPA52379.1"
                     /translation="MELSLTTPALLFPAVSLLLLAYTNRFLALATLVRSLHAHYRERA
                     DPLVLKQIDNLRYRIHLIQAMQATGVSSLLVCVVCMFVLFFGKVVLGKFLFAGSLALM
                     IASLGLSVREIQVSVKALDLHLHDLEERP"
     gene            7146..8297
                     /locus_tag="PLP50_12355"
     CDS             7146..8297
                     /locus_tag="PLP50_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA52380.1"
                     /translation="MTRPFVLVVLSTAVLFFGFYFLIPVLPPYAAARGASKGEVGLVV
                     GLASMTALATRAAFARALDRKGRRPFALGGLALFAAATALLPLAASLVPLLLVRGVQG
                     IGWGLATTAIASLVADLAPAERRGEAIGVWGLAPTVAMALGPAAGGALSRLGGPATAF
                     LGTALLFLLALGLLLPVAEPSHAAGQTRTASLPAGALLPCAVLFLSSLSYGAILAFVP
                     VELGPGKTGTYFLLYAVSILAVRPFAGRLSDRYGRVAVAVPGLLLGAAGVVGTGLARG
                     PLSLAAAAVLYGVGMAGLSFPALVAWTVDLAGEARGTAMAAFYGAYDLAIALGAFALG
                     ALYERHGFAALNLAGAAGVVAASLLAVAFSGAARRRRPDLGNPGPRRAC"
     gene            8306..8683
                     /locus_tag="PLP50_12360"
     CDS             8306..8683
                     /locus_tag="PLP50_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52381.1"
                     /translation="MNVSRLALIVAGLLVLTGIGGYAAFQSPTALIPVGFGVLLGACG
                     LVARKESLRRHAMHAAAAVALLGFVPSVPGLLGIPALLAGEAERPAAVVLRSVMSLLL
                     LGFLVAAVRSFVAARRARPAGES"
     gene            8731..9234
                     /locus_tag="PLP50_12365"
     CDS             8731..9234
                     /locus_tag="PLP50_12365"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPA52382.1"
                     /translation="MRLASATLDSPVGRLLAVVWDGRLCELTFDADATSTREHLLARF
                     GTDLFPSHRDPAGVCTRLRAYFRGDLEALEGVEVDLAGTPFQEKVWAELRRIRPGTTR
                     SYADVAAAVGEPKALRAVGMAAARNPAVLVVPCHRVVASDGSLHGFSGGIERKRWLLV
                     HEGALLA"
     gene            9301..9771
                     /locus_tag="PLP50_12370"
     CDS             9301..9771
                     /locus_tag="PLP50_12370"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HPA52383.1"
                     /translation="MKTTLRATLAAALLAALAATPAAAATKYGKGVSEAAPVKLSELM
                     ANPDAYVGKVVKVEGLITEVCAKRGCWINVAGDVEFQTIRIKVEDGVIVFPMTDKGKK
                     VVAEGTFKKTELTKEQAIAKAKHECEEKGTKFDPASVTGPATVYQIQGTGAVVE"
     gene            9782..10426
                     /locus_tag="PLP50_12375"
     CDS             9782..10426
                     /locus_tag="PLP50_12375"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPA52384.1"
                     /translation="MNWRKLNNVLHRDLGYLAAGLTLVYAISGVALNHRHQWNPSWKL
                     DVEERTFAPVPVSDRETMAAALVERLGLPGPPKSSFRSAPHLVELFYEGWSVKADATA
                     GTATIERPRGRFALREMNFLHLNEPKGLWTWAADVYAVVLALLAVTGLFVLKGKNGLA
                     RRGKWLAGLGVLLPILALFLLLWRPGDSGPRGEGEGNGGGRHRGEGRTEAGPER"
     gene            complement(10428..11606)
                     /locus_tag="PLP50_12380"
     CDS             complement(10428..11606)
                     /locus_tag="PLP50_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA52385.1"
                     /translation="MPPSDPLPFGVPASRDPGPILPTPVRLGADERFRGRGVTIAFLD
                     AGFYAHADLVEPRQRILRYVDVTDPRASYPRLLAPDESSWHGMMTSVVACGNGTLSDG
                     LYRGLASEANVVLVKVGTARRITHENLRRGLEWVVRNRARYGIRVANVSCGGDYEASY
                     LHDALSQAAEKASRSGILVVAAVGNLGLTPGHPVLPPASAPSVLTVGGLDDGNHLHRA
                     GHDIYHGSWGPTVDGLQKPEVIAPAIWVAAPILPGTPTSAEASLHVHLATSRDDELDV
                     ILEAHAGVDPALDAARHLAPPLLRQLVEAKLRDGNVISGAYKHVDGTSFAAPIVSSLA
                     AQMLEANPDLRPHEIKSILVSTARRLPHVAVDQQGWGVVEPADAVEEALARAGAGPAA
                     "
     gene            11752..13446
                     /locus_tag="PLP50_12385"
     CDS             11752..13446
                     /locus_tag="PLP50_12385"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPA52386.1"
                     /translation="MPQHFRALALDYDGTLTSTGRLDETVLAALRTLRQERMKLVLVT
                     GRIPWELLELCPHAEELFDRIVWENGAVAWGARGARPLAPPVPGELEEALARRGVALR
                     RGQAILAGHARDAATALEEIGRLGLEVQVVRNRQEMMLVPSGVSKGTGLFDALGDLGI
                     SRHSTVAIGDAENDHSLLGACELGVAVADAVPSLRAAADVVLPEAGSAALARFLLGPA
                     VLSDETPEPRRFQAVLGTFEDGSPARLPASGVNVLVTGGSMSGKSYLTGLLAERLLAL
                     GYSLCVLDPEGDHVAVGRLRGVLAVGGNEPLPGPDQLPRLIEHRFGSLVVDLSFLDPS
                     EKTAYARAALVELSALWNDTGLPHWLFLDEAQVALNLAHLPVEGFDPSRPGTCLTTWR
                     PEDLPPDVVGSLDVVLDLGAEETTGLPEWARSAEARPPPGQALLSRRSRPGPRRFTVS
                     RRASPHARHLHKYIGGSLPPEQRFFFRSGAGATGRVAGNVEEFHRELRRAEADVVRHH
                     ALHGDLSRWVGDVLRDEVLAATVRTAERALRHRASAYEIESLRTEVLAAILDRYQG"
BASE COUNT         1684 a         4822 c         4963 g         1962 t
ORIGIN      
        1 cccttgccgt ccttgtcgcc ggggtaccag ttctggacgg tggagtactt gatcttcgcg
       61 tcgtccagcg cgacgagctc gacgacggcg gcgtggagct ggttcgtgtc gcgcttcggg
      121 gcggtgcacc cctcgaggta cgacaccgag gcgccttcct ccgcgacgat cagcgtccgc
      181 tcgaactgcc ccgtgtcggg ctggttgatc cggaagtagg tcgacagctc catcgggcag
      241 cggaccccct tcggcacgaa gcagaaggag ccgtccgaga agaccgcgga gttgagcgtc
      301 gcgaagaagt tgtcggtgta ggggacgacc gagccgaggt acttcttcac gagctccggg
      361 tgctcctgca ccgcctcgga gaaggagcag aagatgacgc cgaccttcgc gaggctctcg
      421 cggaacgtcg tcgcgaccga cacgctgtcg aagacggcgt cgacggcgac gcccgcgagg
      481 atcgcccgct cctcgagcgg gacgcccagc ttctcgtacg tgcggagcag ctcggggtcg
      541 acttcgtcga gcgtcttcgg tccgtccttc gtcgacttcg gcgcggcgta gtagcggatc
      601 gcctggtagt cgatcgcggg gtaggagact ttcgcccacc tcggctcggt catcgtctgc
      661 cagtggcgga acgccttcag ccgccagtcg aggagccact gcggctcctt cttcttcgcg
      721 gagatggccg ccacgatctc ctcggtcagg ccgggaggga agacgtcgga ctcgatctcc
      781 gtgacgaaac cgtgccggta ttcctggctc gtcagctcgg agaggacctc gttcgagctc
      841 atgaagcgat gctcctgggg gcggaatcga ccgccggaag cggggacccg gtgcgggtcg
      901 cccccgacga cgccgggcgc gtcatgtcgg cgagggtcag gcgcgagagc gcgtcctgga
      961 tgacgtcgtt cacggccttc cagttcggac gggcggggca gccccgctcg tgggcgcagg
     1021 tcccgggaag gctgcactcc gtgatcgcga gcggcccttc gagcgcctcg atgatcgcgg
     1081 cgaccggaat ctcctccggc gacctcgcga gcgcgtagcc ccccttcgtc ccgcgctggg
     1141 agacgaggag gcccgccttc agcagcgtct tcaggagctt cacgaccgtc ggctgcggga
     1201 tctcggtccg gacggagagc tcgcgcgcgc tgatgccgag cgccggctcg tgcgcgaacg
     1261 tcgtcaggag gacgaccccg tagtcggtga gcttgttcat tcggatcatg tctggtgctt
     1321 cctgccgccg ccccaatcga gacgcgggct caattaggac ctctccagta ctgtatttcc
     1381 ggagaccgga ggtgtcaagc gttgaagggc gggccggaag gggtgaaagg cgaagcgccc
     1441 cggctccttt cggcgccggg gcgcgagatc cggagcgagg tccccgtcag gacagcttga
     1501 acgagacggt gaccgtgagg aagaccttca cgggcttccc cttcttcgtg gccggcttgt
     1561 aggtccactt cttcacggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1681 cttcacgggc ttccccttct tcgtggccgg cttgtaggtc cacttcttca cggcgtccag
     1741 cgccgattcg gtcagcatcg gatcggacga ctcgaccgct tcgaccttcg tgacgactcc
     1801 cttctcgtcg atgacggccc ggacgacgac gcgcccctgg acccggttct ttcgcgcctc
     1861 ctcggggtag gtcggctgga cgcgctgaat ctcgaccggc tccttcacat cgccgccgac
     1921 cttgaggatg acgtcgtcgc cggttccctc cgcgacgccg ccggccgcgc ctccccacac
     1981 gcccccggcg acgccctccc ggacggcgtc cgacggcgag gccgcggacg aggcggcggg
     2041 ctccgccccg ggatcgcccg cggagcttcc gaacgggacg acggcggctc cggcggcgcc
     2101 gacgaggagg aggagggccg ccgcggcggt cgtggcgagg acgaggcttc tgcgtgacat
     2161 ggggacctcc tttgcgaggt gggccaccct ccgaaggagg tggctccgcg tgtggaaggg
     2221 gagcgccccc gagggggcct cctgggcgaa ggcggcgagc cgggcgagcg cacggaggta
     2281 ggagcggcga tcgccggtgg atcggacgac gtcgccgtcg atcgcctgct cccgcgcgag
     2341 agagatgccc gagaggagcc cccacgcggc cggctgcgac cagaggagcg cccgcgcgag
     2401 ctcctcgagg aggacggccc gggagtcgcg gcgtttcacg tgcaggagct cgtggcaggc
     2461 gacagcctcc tgctccgagg ccccgagcgc cgcgaagcgc tccgggagga tcacggccgg
     2521 gcgtcgccat ccgatcgcga cgggggaggc gacctcggag gagacgagga gctcggcgca
     2581 cgcccccgtc cgctcctcgg cggagacgac ggatgccggg acgggggcga gggggcgcgc
     2641 tctccggcgg agggcccgca ggctcaggag ccccgccccg agccagccga gccgaaggag
     2701 ggcgcccgcg gagagcgccg cgaggacggc cgtttcgagg gagggcgcgc cggccgtcgc
     2761 gccgggctcg acggggacga cgagcgagaa cgccgtccgg atgctggcgc cgccggcccc
     2821 atccgcgggg cgcggaggct ggacgaaggg gagggcgaag gccgcgagca gcgccgcctg
     2881 cgcgatccgc agccgcgccg ccgggtcgcg cagccggacc gccgcgggga cgaggagcgc
     2941 ggcgccgagg aggagccccg actggagcca ccaggaggcg aggtcggcga ggaggggcgt
     3001 catcggtcgt gctcctccgc gagccgcgcg atctcgtcga gctcgcgctt cgagatcttc
     3061 cggtccttca ggagcgcgag gacgagcggc cgggcggagc cacggaagac ccgctccacg
     3121 aagtcgtcga gcatccgcga gacgacgcgt cccttcgggt gcgccggccg gtagacgaag
     3181 gccctccccg actcctcccg cttcaggtgc cccttcttcg cgaggaggcc gagcatcgtc
     3241 atgaccgtcg tgtaggcgac ggggcggcgg gcggagagga cgtcgtggac ctgcctcacc
     3301 gtcgcgtcgc cgagctccca gacgacgtgc atgatctcga gctcggcttc cgtgaggggc
     3361 gtcggtacgg gtcgcatagt actaagaact tagtactaca tgcttcggat gtcaagggcc
     3421 gggcttccga gcccggtccg gggaaaaccc ggcgcgtccg cccccgtatc ctgagggcat
     3481 gtccgtcgcc gcgaggtccc cgtcctgacc cccgctctcg aggtctccgg cctccggaag
     3541 tcgtacggcg ccgtccaggc cgtcgccggc gtgtcgttcc gcgtcggagg agggacgatc
     3601 gtcggcctcc tcgggccgaa cggggccggc aagacgacga ccgtctcgat cgtctgcggt
     3661 ctcgtccctc ccgatgcggg agaggtgaag gtcgggggcg tcgccctttc gggggacacc
     3721 gacccggcga aggggcgtct cggcctcgtg ccgcaggagc cggcgctcta cgaggagctg
     3781 acggccgagg acaacctccg gttcttcggg gcgctccagc gaatggaggg ggcccgcctc
     3841 gcctcccgga tcgacgccgt ccttcggctc acggggctcc tcgagcgccg ccgggaccgc
     3901 gtgaaggtct acagcggcgg aatgaagcga cgcctgaacc tcgcggcggg gctgctgcac
     3961 gaccccgaag tcctcctcct cgacgagccg acggtcggcg tcgaccccca gagccggaac
     4021 gcgatcttcg agagcctcga ggcgctccgg gacgccggga aggcgatcct ctacacgacg
     4081 cactacatgg aggaggccga acggctctgc gaccgcatcg tcatcgtcga ccgcggccgg
     4141 gtcgtggccg aggggacgaa ggacgagctg ggcgcgctcc tgccgggcaa cggcgctcgc
     4201 gatctcgacg acgaggcgcg cgccgccgtc gcgctcctcg ccgctcgggg gatcgccgtt
     4261 cccggagcgg acggcgggac gccgcggctc gagaaggtct tcctccacct gacgggccac
     4321 gccctgaggg acgagccgtg aagcaggccc tcgcgatcgt cgtccacgag ctgaagctct
     4381 tcctctccga ccggcgatcc gtcgtcgtcg ggatcgtgac gccgatcctt ctggcgacgt
     4441 tcttcggctt cctcttctcg ggcgcggggc ggcaggacgc ggcgaagatc ccggtcctcg
     4501 tcgccgacgc ggacgacagc gccgtgtcgc gcgcggtcgt ggcggggctc gctgccgacg
     4561 cgctcctctc cgtgagcccg gcggccgcgg aggacgcgcg gcaggccgtg cggagaggga
     4621 aggccgccgc gggcgtcgtc ctgccgaagg gcttcggcga aggcgccgtg caggcgttct
     4681 tcggcgccgc ggcggcatcc gagatcgtcc tcctcgtcga tccctcgcgc gcgacggagg
     4741 ccggcctcgt gcggggcctc ctcgccgaga aggtcttccg cgaggcatcg cgggaggcct
     4801 tcggcggggc gacggggacg aaggtcctcg aggagagcct ggtcgcgctc gagtccgatc
     4861 cgtcgcctcc gacggccgag cgtcaggcac tgaaggacct gctgcgcgac gtgacgcgct
     4921 ggcagggggc ggcgaagggc ggaacggccg ggggcggcgc ggcgggcggg ctggggacgg
     4981 cggtcccgtt caccctgaag gaagagccgg tcaccgcgcg gcagggggtg acgtacaaca
     5041 gctacgcgca ctccttcgcc gggatggcga tccagttcct cctcttcgtc gcgatcgact
     5101 tcggcatcgc gctcctcctg gagcggcagg gcgggctctg gaagcgcctc cgttccgcac
     5161 cgctctcccg ctcggctctc ctcctcggaa agacggccgc cggggcgctc gtcggcctcg
     5221 tctccgtcgc ggcgacgttc gcgttcggca tcgtcgtcct cgggatccgg atcgagggga
     5281 gtgttccggg cttcgtcctc ctcctcctcg cgtcggcgct gatggcctcc tcgttcggcc
     5341 tcctcctcgc ggcactggga aggactccgg gggccacgcg cggcctcgcg atcctcgccg
     5401 tcctcctcct cgtgatgctc ggcgggggct gggtccccgc gttcgtcttt ccggcgtggc
     5461 tgcagaaggt gacgctcgtc ctgccgacgc gctgggcgat cgacggcctc gacgcggtga
     5521 cgtggcgcgg cgccggcctc gcggccggcg cccttccggc cgtcgcggtc ctcctcggct
     5581 acacggtcct gttcctcctc gtcgcgcgcc tgcgcttccg ctggaccgac cggtagcccg
     5641 ccagtcccgg cgccttccgg accgggtcgc ccaggggaga cccccgcatc tgtgccaggc
     5701 gtgaattcgc ggggagtggt gccaggcgtg aaatcgtgta ttgttttcaa caatacacga
     5761 tttcacgcct ggcaccgagc cccgggttcc gaggccacgg ggcgccggac tcgcgggccc
     5821 tccgctccgc ccgccgtcga cgcgtccttc ctcggggacg agggcctcag cggagccgga
     5881 acgtgagcgt gactcggagg acgacggaga cggggcgcgc gccgaggcgg gccggcgtat
     5941 agcgccagct cgagacggcc tcgatggcga gccgctcgag gacggggtgc gcatcgttct
     6001 cgaccgcgac gtcgacgacc ctgccgtcgg aggcgatcgt cgccgagagg acgacgacgc
     6061 ccgcggcgcg gacgatgcgg gcggcctcgc tgtaggcggg cgcgggggtg tcgatcggca
     6121 ccggcaggac gaggtcgggc gggagcgctc cgccgcggac gtcgtacagc ggggcgcctc
     6181 ccgggtcttc gtcgccgacg ccggtcccgg ccgtggtcga cgtcccgtcg tcgaagcctc
     6241 cgggaacgcc cggctcgccg tcgggctcga acggcccggc gggcgggagc tcgtcgggga
     6301 tccgcccggg ctgggtcgcc ggccggagag gtcgagcgac ggtcccggga cgggccgcga
     6361 caggaacgcc gcctccgcgg ggcgcgcctc cggagcgcgc cggcgccggg cgctcgcgca
     6421 ggtcgacggt gcgggtctcc cggaggaccg acaggtcggg ctgcgtgacg gcccagacct
     6481 cgccgcgcca gccggggacg tcggggaaga ggaggagcgg gccgaaaccg aggaccgaga
     6541 aggccgtcag gtggaggccg atggaggcga ggagagcggg gcggggccgg tcggggggcg
     6601 tcgcgggctg gagcatcgtc gtcctcctcc gtcgttttcc ggtgagaccc ggagggaggc
     6661 gcggagttcc cggcgccccg gcggacgcac cgctccggac gccgggcccc gggtctcggc
     6721 tatcgtccgg gcgccatgga gctctccctc acgacacccg cgctcctctt ccccgcggtc
     6781 tcgctcctcc tcctcgccta cacgaaccgg ttcctcgcgc ttgccacgct cgtgaggagc
     6841 ctgcacgccc actaccggga gcgggccgac ccgctcgtcc tgaagcagat cgacaacctc
     6901 cgctaccgga tccacctgat ccaggcgatg caggcgacgg gcgtctcgag cctcctcgtc
     6961 tgcgtcgtct gcatgttcgt cctcttcttc gggaaggtcg tcctcgggaa gttcctcttc
     7021 gccggcagcc tcgcgctgat gatcgcttcg ctcggcctct cggtgaggga gatccaggtc
     7081 tccgtgaagg cgctcgacct ccacctccac gacctcgagg agcgcccctg acggaccggc
     7141 ggatcctgac ccgtccgttc gtcctcgtcg tcctctcgac ggccgtcctc ttcttcggct
     7201 tctacttcct catcccggtc ctcccgccgt acgcggccgc gcgcggcgcc tcgaagggcg
     7261 aggtcggcct cgtcgtcggc ctcgcgtcga tgacggccct cgccacgcgt gccgcgttcg
     7321 ctcgcgccct cgaccggaag gggcggcgac ccttcgctct gggcggactc gcgctcttcg
     7381 cggcggcgac ggcgctcctc ccgctcgccg cctcgctcgt gccgctgctc ctcgtgcggg
     7441 gcgtgcaggg gatcggctgg ggcctcgcga cgacggcgat cgcgtcgctc gtcgcggacc
     7501 tcgccccggc cgagcggcgc ggcgaggcga tcggcgtctg gggcctcgcg ccgacggtgg
     7561 cgatggcgct cggcccggcc gcgggcgggg cgctgtcgcg actcggaggg cccgcgaccg
     7621 cgttcctcgg gacggcgctc ctgttcctcc tggcgctcgg cctcctcctg cccgtcgccg
     7681 agccgagcca cgcagcgggg cagacccgca cggcgagtct cccggccggc gcgctcctgc
     7741 cgtgcgcggt cctcttcctc tcctctctgt cgtacggcgc catcctggcc ttcgtcccgg
     7801 tcgagctcgg gcccgggaag accgggacct acttcctcct ctacgcggtg tcgatcctcg
     7861 cggtccggcc gttcgcgggc cgactctcgg accggtacgg gcgcgtcgcc gtggcggtgc
     7921 ccggcctcct cctcggagcc gccggagtcg tcgggacggg cctcgcgcgg ggtccgctct
     7981 ccctcgccgc cgccgccgtc ctctacggcg tcggcatggc gggcctctcc ttcccggccc
     8041 tcgtcgcctg gaccgtcgat ctcgccgggg aggcgcgggg aactgcgatg gcggcgttct
     8101 acggcgcgta cgacctggcc atcgcgctcg gcgcgttcgc cctcggcgcg ctctacgaga
     8161 ggcacggctt cgcggctctc aacctggccg gcgcggcggg ggtggtcgcc gcctcgctcc
     8221 tcgcggtcgc gttctccgga gccgcgcgac ggcgccgtcc ggatctcggg aatccggggc
     8281 cccgtcgcgc gtgttagcgc ttctgatgaa cgtctcccgt ctcgccctca tcgtcgccgg
     8341 cctcctcgtc ctgaccggaa tcggcggcta cgccgcgttc cagagcccga cggccctcat
     8401 cccggtcggg ttcggcgtcc tcctcggagc ctgcggcctc gtcgcgcgga aggagagcct
     8461 ccgcaggcac gcgatgcacg ccgccgcggc cgtggcgctc ctgggcttcg tcccgtcggt
     8521 ccccgggctc ctcgggattc cggccctcct ggccggagag gccgagcggc ccgcggccgt
     8581 cgtcctccgg agcgtcatgt cgctcctcct cctcgggttc ctcgtcgccg ccgtccggtc
     8641 gttcgtggcg gcgcggaggg cgcgcccggc cggggagagc tgagaccgcc gatcccgcgc
     8701 gcggcggcga aatggggtag cgttccgggc gtgaggctcg cgagcgcgac cctcgactct
     8761 ccggtcgggc ggctcctcgc cgtcgtctgg gacgggcggc tctgcgagct gacgttcgac
     8821 gccgacgcga cctcgacgcg agagcatctc ctcgcccgct tcgggaccga cctcttcccg
     8881 tcgcaccgcg accccgcggg cgtctgcacg cgcctcaggg cctatttccg gggggacctc
     8941 gaggcgctcg agggcgtgga ggtcgatctc gccgggactc cgttccagga gaaggtgtgg
     9001 gcggagctcc gccgcatacg gccggggacg acccgctcct acgccgacgt cgccgccgcc
     9061 gtcggcgagc cgaaggcgct gcgcgcggtc gggatggccg cggcgcgcaa cccggcggtc
     9121 ctcgtcgtcc cctgccaccg cgtcgtcgcg agcgacggga gcctccacgg cttctcgggc
     9181 gggatcgaac ggaagcgttg gctcctcgtc cacgagggag cgctcctggc ctgacgcgcg
     9241 tccgggcggg ccgacggtag aatccgccgc tcgaacgccc gacagcagga gacacgaccg
     9301 atgaagacga ccctccgcgc gaccctcgcc gccgccctcc tcgcggccct cgccgccacg
     9361 cccgccgccg ccgccacgaa gtacggcaag ggagtctccg aggcggctcc cgtgaagctc
     9421 tccgagctga tggcgaatcc cgacgcctac gtcggaaagg tcgtgaaggt ggaggggctc
     9481 atcaccgagg tctgcgcgaa gcgcggctgc tggatcaacg tcgccggcga cgtggagttc
     9541 cagacgatcc ggatcaaggt ggaggacggc gtcatcgtct tcccgatgac cgacaaggga
     9601 aagaaggtcg tcgccgaggg gacgttcaag aagacggagc tgacgaagga gcaggcgatc
     9661 gcgaaggcga agcacgagtg cgaggagaag gggacgaagt tcgacccggc ctccgtgacc
     9721 ggcccggcga ccgtctacca gatccagggg accggcgccg tcgtcgagtg agcgttccgc
     9781 cttgaactgg cggaagctca acaacgtcct ccaccgcgac ctcggctacc tcgcggccgg
     9841 gctgacgctc gtctacgcga tctccggcgt cgcgctgaac caccggcacc agtggaaccc
     9901 gagctggaag ctcgacgtcg aggagcggac gttcgccccc gtgccggtct cggaccggga
     9961 gacgatggcg gcggcgctcg tcgagcgcct cgggctgccc gggccgccga agtcttcgtt
    10021 ccgctccgcg ccccacctcg tcgagctctt ctacgagggg tggagcgtga aggctgacgc
    10081 gacggcgggg acggcgacga tcgagcgtcc gcgcggccgc ttcgcgctcc gcgagatgaa
    10141 cttcctccac ctgaacgagc cgaaggggct ctggacctgg gcggccgacg tctacgcggt
    10201 tgtcctcgcg ctgctcgccg tgacgggcct cttcgtcctg aaggggaaga acggcctcgc
    10261 gaggcgcggc aagtggctgg cgggcctcgg cgtcctcctg ccgatcctcg cgctcttcct
    10321 cctgctctgg cgcccgggcg actccgggcc gcgaggggag ggcgagggga acggaggggg
    10381 ccgccaccgc ggggaagggc gcacggaggc cgggccggaa cgctgagcta cgcggccggg
    10441 ccggcgccag cgcgcgcgag cgcctcttcc acggcgtcgg ccggctccac gacgccccag
    10501 ccctgctggt ccacggcgac gtgcggcagc cgccgtgctg tcgacaccag gatcgacttg
    10561 atctcgtgcg ggcgcaggtc gggattcgcc tcgagcatct gcgcggcgag ggaggagacg
    10621 atcggcgccg cgaagctcgt cccgtcgacg tgcttgtagg ccccgctgat gacgttgccg
    10681 tcgcgcagct tcgcctcgac gagctgccgg aggagcggcg gcgcgaggtg ccgggccgcg
    10741 tcgagcgcgg ggtcgacgcc ggcgtgcgct tcgaggatga cgtccagctc gtcgtcgcgc
    10801 gacgtggcga ggtggacgtg gagcgacgcc tcggcggacg tcggggtgcc ggggaggatc
    10861 ggcgccgcca cccagatcgc gggcgcgatc acctcgggct tctggagccc gtcgacggtc
    10921 ggaccccagc tgccgtggta gatgtcgtgc ccggcgcggt ggaggtggtt gccgtcgtcc
    10981 aggccgccga ccgtcaggac ggacggcgcg gaggcgggcg ggaggaccgg gtggccgggc
    11041 gtgagcccca ggttcccgac ggccgcgacg acgaggattc ccgaacggct cgccttctcg
    11101 gcggcctgcg agagggcgtc gtggaggtag gacgcttcgt agtccccccc gcaggagacg
    11161 ttcgcgacgc ggatgccgta tcgcgcgcgg ttgcggacga cccattcgag gccgcggcgg
    11221 aggttctcgt gcgtgatgcg ccgggccgtc cccaccttca cgaggacgac gttcgcctcc
    11281 gaggcgagtc cgcggtagag gccgtcggag agcgtgccgt tcccgcaggc gacgacggag
    11341 gtcatcatcc cgtgccagct cgactcgtcg ggcgcgagga gccgcgggta ggaggcgcgc
    11401 gggtcggtga cgtcgacgta gcggaggatc cgctgccgcg gctccacgag gtccgcgtgc
    11461 gcgtagaacc cggcgtcgag gaaggcgatc gtcacgcccc ggccgcggaa cctctcgtcc
    11521 gcgccgagcc gtacgggcgt cgggaggatc ggtcccgggt cccgggaggc cgggactccg
    11581 aacgggaggg ggtcggaagg cggcacgctc cggagacgct agcaggttgc cggggaaatg
    11641 ggaggggccc gtcgcgagcg ccgcgggccc ggggtccgtc ccgggtgata gagtttcggc
    11701 cgagagtccg tcgtcgtccg taccggcccg ttcgccctcg cgggagggga gatgccgcag
    11761 cacttccgcg cgctcgcgct cgactacgac ggcacgctga cgtcgaccgg gcgcctcgac
    11821 gagacggtcc tcgccgcgct ccgcaccctt cggcaggagc ggatgaagct ggtcctcgtc
    11881 accgggcgca ttccgtggga gctcctcgag ctctgcccgc acgccgagga gctcttcgac
    11941 cggatcgtct gggagaacgg cgcggtcgcg tggggcgcgc ggggcgcgcg cccgctggct
    12001 ccgcccgttc ccggagagct cgaggaggcg ctcgcccggc gcggcgtcgc gctccgccgg
    12061 gggcaggcga tcctcgcggg gcacgcgcgc gacgcggcca cggcgctgga agagatcgga
    12121 cggctgggtc tcgaggtgca ggtcgtgagg aaccggcagg agatgatgct cgtcccgtcg
    12181 ggcgtctcga aggggaccgg actcttcgac gcgctcggcg acctcggcat ctcgcgccac
    12241 tcgacggtgg cgatcggcga cgcggagaac gaccactccc tgctcggcgc gtgcgagctc
    12301 ggcgtcgcgg tggccgacgc ggtgccgtca cttcgcgccg cggcagacgt cgtcctcccg
    12361 gaggccggaa gcgcggcgct cgcccgattc ctcctcgggc cggccgtcct ctccgacgag
    12421 accccggagc cgcgccggtt ccaggccgtc ctcgggactt tcgaggacgg ctctcccgcg
    12481 cggctcccgg cttccggggt gaacgtcctc gtcacgggtg gctcgatgtc ggggaagtcg
    12541 tacctgacgg gcctcctcgc cgagcggctc ctcgcgctcg gctactcgct ctgcgtcctc
    12601 gacccggaag gggaccacgt cgcggtcggg cgtctccgcg gggtcctcgc cgtcgggggg
    12661 aacgagccgc tccccggccc ggaccagctc ccgcgcctca tcgagcaccg gttcgggagc
    12721 ctcgtcgtcg acctctcctt cctcgatccg tccgagaaga ccgcctacgc gcgcgccgcc
    12781 ctcgtggagc tctcggcgct ctggaacgac accgggctcc cgcactggct cttcctcgac
    12841 gaggcgcagg tcgcgctgaa tctcgctcat ctcccggtgg agggcttcga cccgagccgt
    12901 ccggggacgt gcctgacgac gtggcggccc gaggacctgc cgccggacgt cgtcggaagc
    12961 ctcgacgtcg tcctcgacct cggtgccgag gagaccacgg gcctgccgga atgggcccgt
    13021 tcggccgagg cgcggccgcc gccggggcag gccctcctgt cccggcggag ccgtccgggg
    13081 ccgcgccgct tcacggtctc gcgccgggcc agcccgcacg cccgccacct ccacaagtac
    13141 atcggcggct cgctccctcc cgagcagcgg ttcttcttcc gttccggcgc gggggcgacg
    13201 ggacgcgtcg ccgggaacgt cgaggagttc caccgcgagc tccggcgggc cgaggccgac
    13261 gtcgtccgac accatgcgct ccacggggac ctctcgcggt gggtcggcga cgtcctgcgc
    13321 gacgaggtcc tcgccgcgac ggttcggacg gcggagcgtg cgcttcgcca ccgtgcctcc
    13381 gcctatgaga tcgaatcgct ccggaccgag gtcctcgccg cgatcctcga ccgctaccag
    13441 ggctaggaaa cgtcgcgcgc gaggctaaac tcgccctcat gatcgacgca cgcgcacgcc
    13501 gtgaccggat cgctcagtgg gccttcgacg c
//
LOCUS       DAOONL010000088        13390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5072_length_13390_cov_15.140907, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000088 DAOONL010000000
VERSION     DAOONL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13390
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_13390_cov_15.140907"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLP50_12390"
     CDS             <1..888
                     /locus_tag="PLP50_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPA52387.1"
                     /translation="RPFVTHHNALDVDLYLRIAPELYLKRLITGGFPKVYEINRNFRN
                     EGISTQHNPEFTMLEFYTAYADARDQMALTEELLAGAAREILGTTQLPWGDETISFEA
                     PFRRLSMKDAVVEYGRDVPGGAIRADELATLEGLKGAAERAGVESIERFGDVPGKLLA
                     ELFETLAEPNLLQPTFVVDYPAEISPLAKTRPDDPRTADRFELFVGRMELANGFSELN
                     DPDEQAEKFRAQVAERTKGDDEAMLFDEDYVEALSYGMPPTAGEGVGIDRLAMLFTNS
                     RSIRDVILFPLMRPKAAGR"
     gene            942..2033
                     /locus_tag="PLP50_12395"
     CDS             942..2033
                     /locus_tag="PLP50_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52388.1"
                     /translation="MGVVALAAFVGLVLGVAALVGALALLSGFQSHVLSRLADETPHV
                     LVTPAGRPDFAGEEGAGRTLAALPGVVSVVPVVRGRGWITARGQAVPAALTGREGADG
                     ITLDPSQSRQLSAFPGEEVVVVSSRSRLSPIGPVPITASLPVTDVAVASTGRHQPEAV
                     LPADAARRLFGLPSAGATGYELRLADADAAADVAARARAALGETATATTTWQEANRPL
                     LLALKLERLAIFATVFLVVVVAGLNLAATSAVLAATRRGDAAVLTVLGAGPALLARVF
                     LLAGLLLGAAGTLCGLAVGAILAIVLDATKALPLPAQLFSLAHVPFRVTARDLLAVGT
                     FSLLWSFVASLVPARMAARVDVAEALRAG"
     gene            2023..2706
                     /locus_tag="PLP50_12400"
     CDS             2023..2706
                     /locus_tag="PLP50_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52389.1"
                     /translation="MPAEPILRAESLAKGFGEAEARVELFSGLSLELGAGEATAIVGP
                     SGCGKSTLLHLLAGLDRPDAGRVLVDGRDWTALAPEPRARWRGEAVGFVFQLHHLLPE
                     LTAEENVALPLLLAGSPEGAARAAARELLSRVGLSRQSGAFPRVLSGGERQRVAIARA
                     VVRSPRLLLCDEPTGSLDAAHAADVLELLLGAARERAGALLLVTHDPGVASRCGSVRT
                     LTPDGLSAA"
     gene            2793..5219
                     /locus_tag="PLP50_12405"
     CDS             2793..5219
                     /locus_tag="PLP50_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPA52390.1"
                     /translation="MFEKYNEKARRALFFARYEASKLGSKVIESEHVLLGILREGEDV
                     TREIFARFNVKPDDVRKEIEGDRVFVERVSSTQELPLSEESKKILAYASHEAESMMHP
                     YVGTEHLLIGILRVDPCVASRLLTAHGFNLYGVREEAIALIKEREASKQKKELPFLAE
                     YARDLTALAAQGTFDPLVGRDKEVERIIQILSRRTKNNPILLGEPGVGKTAIVEGLAQ
                     RIVEGAVPLFLANRKILAMDLSLIVAGTKYRGQFEERLKGILKELRENQDLIIFIDEI
                     HSLIGAGSAEGSLDAANILKPALSRGEIACIGATTLKEYRKYIEKDRSLLRRFQAITV
                     NPPSEEETLEILEGVRERYERFHRVRYAPDAVKTAVYQSSRYIPDRFFPDKAIDLLDE
                     AGAKVKLRMTADTQNLRRLENEVNQIVKEMKKAISDKDFEHAVSLREREVEIKEEIER
                     TRNAQLDEGAWAEVVRGDIEEIIASWTGIPVTSIQVEEAEKLLNMETTLRKRVIGQDR
                     AIDAISRAIRRSRLGVKNPNRPMGSFIFLGPSGVGKTEVARRLAEFLFDNQKALVRFD
                     MSEYMEKHAVSKLIGSPPGYVGHEEGGQLTERIRRQPYSVILLDEIEKAHPDIANLLL
                     QILEDGQLTDAYGNTVDFKNTLIIMTSNIGTKYLVTQARVGFGDGKNAPPAREVEEMV
                     LKELRREFSPEFTNRIDEVIVFNPLTTENLRAICRLLLDDVAQTLKHRGLELVADEKA
                     VDWLLSMSGQDAHSGARPLRRTIQRHVEDAVSELLIGAREQLECLEVTVIESELKVLV
                     RSAAALATER"
     gene            5234..7834
                     /gene="bamA"
                     /locus_tag="PLP50_12410"
     CDS             5234..7834
                     /gene="bamA"
                     /locus_tag="PLP50_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPA52391.1"
                     /translation="MYIDPDRGAGAPGWPLGARRPAGRVRVAAILALFLGGAVPAPAQ
                     TQGEPPAAPAAPPATLDLRPGAAGPQAPPSPLAGALPPAPALRTSPGADGRERIAEIA
                     VRGGRTVTAETIAFYLGVKVGDPYDTEALRRAFPKLWESGLFEDLRLERESVPGGSRI
                     VAVVVERPRIGDLEFRGNKKLTTSQLKDKLKEGKVEIRVGSPLSLSDVSKGKGVLMEA
                     YQADGFRSAVVDTALEPMGENEKRVVFLVDEGDKIKIEAIDFTGNTVFSDRRLRFAMK
                     KTRQRNFYQFWDSKDVYNQANYEEDIESVKKVYQNAGYKDIVAKDPVIETFVVNPDET
                     RRDKIKRRARIEIPLVEGERFYFGDVKVEGTTVFPGDRLLQFFEWSPGKPLKRSALVD
                     GMKALDEIYRGRGYIYAFMNPEYTERPGEGNLVDVVVRVTEGDMFRVGRVEFTGNKTT
                     KDKVLRREIPLFEGDVLDMESFKKGLLKLSQLGYFKIEEDPDFRVDPEKKVVDVTIKG
                     LDTNRNEIQFGAGYSQLDGVFGQFQFATRNFLGRGDTISVQFQRGGYSNFFDLSFVEP
                     WFLDKRMSVGGSVFNRSLEYLDVDQQTKGVNMSFGLGLGLFDGLSLFYGYTDTKSRYQ
                     VYPAAPPPGSGIPPAAYADYIGKTSAIAPGYRYDSRNDPFDPSRGRRFNFSTTIAGGP
                     LGGNFSYIKPVGGATSYFRTGRKSHFAVNMEGGFIRAYDGKEIPIFERFRIGGDRSVR
                     GFQYGSIYPLDDDDKAFFTDQGALLGGDKYFVFNAELVHAIAGPLKLVLFFDAGNAWL
                     EGQSFNPLKARASTGAELRMFLPIFQAPLRFIYGINLDPKTIKGPDGSPLTGGAEKSS
                     DFQFSIGTTF"
     gene            8144..8725
                     /locus_tag="PLP50_12415"
     CDS             8144..8725
                     /locus_tag="PLP50_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPA52392.1"
                     /translation="MIGNRMFVAGVVATAALVALPASAQSAQKVGVIEVQRIVQDSAV
                     GKESLARIQKVSTAKQEDLTKRQTELRDLEKRIQEQGKSLSEEAMEKLQKDYQQKALD
                     LKRFQDDAQRELEELQRRELTDLEKKVLPVIEAVSKELGYTLVFNKFQSGLLYADDAV
                     DLTAAVIQKFNTAIAAAPKTDAKPAAATPAPKK"
     gene            8733..9764
                     /gene="lpxD"
                     /locus_tag="PLP50_12420"
     CDS             8733..9764
                     /gene="lpxD"
                     /locus_tag="PLP50_12420"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPA52393.1"
                     /translation="MARFPAPTTAAAVASACGGRLVGDAGTVVEGARSLESAGPADLA
                     FAADAKAEQAAATSAAGVLLARSALGFPGRAVVEVPDPALAFAGVLEAFFSRRAPAPG
                     IHATAIVEAGARVAEDATIGPYAVVGAETEIGSGAVLGAHVVLGRRCRVGAGAWLHPH
                     VVLYDDVSIGPGSEIHAGAVLGADGFGYVASPTGLKKVPQVGGVEVGSSVEIGANTCV
                     DRATLEATRVGDGTKIDDLVMVGHNCEVGAHVILCGQVGLAGSTTVGDQAVLGGQVGT
                     AGHLRIGRGVKAGGQTGIVGDVPDGASLFGTPHLPHRDAFRIHAELKKLPETARLVRE
                     LARAGEEKR"
     gene            9761..10237
                     /gene="fabZ"
                     /locus_tag="PLP50_12425"
     CDS             9761..10237
                     /gene="fabZ"
                     /locus_tag="PLP50_12425"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPA52394.1"
                     /translation="MSEAPTDRGGLDIRAILRTLPHRYPFLLVDRILEIVPGQKVVGL
                     KNVTINEEFFQGHFPGNPVMPGVLLVEAMAQVGGFMMLAEVDDPQSRQVRLTGLDAVR
                     FRRPVVPGDQVVFTVEFVARKKSLVKVRGEARVDGQLCVEAEIMSLIQDCGREEGD"
     gene            10238..11017
                     /gene="lpxA"
                     /locus_tag="PLP50_12430"
     CDS             10238..11017
                     /gene="lpxA"
                     /locus_tag="PLP50_12430"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPA52395.1"
                     /translation="MPIDRTAIVHESATVHPDAVIGPYAVVGASVVIGARTRIGAHAV
                     VDGPTVLGEDNVVHPHAALGGPPQDLKYRDEPTRLVVGSRNVFREFMTAHRGTVTGHH
                     ETVIGDDNLFMAYSHVAHDCIIGSRTVFANSASLSGHVTVEDDVILGGFVVVKQFLRI
                     GRFAYVGGMTPVNKDVLPFTWTSSDRDTKAYKINGVGLSRKGYSAERVAALNKAYRIL
                     YRNRHDHAAALEALAALAATAPDVQVLLDFISSSKAGVHGA"
     assembly_gap    11163..11262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11379..>13390)
                     /gene="fusA"
                     /locus_tag="PLP50_12435"
     CDS             complement(11379..>13390)
                     /gene="fusA"
                     /locus_tag="PLP50_12435"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPA52396.1"
                     /translation="ALLHEAGAVNRFGRVDDGSAPTDFDPEEIERKISIGLAVAALEW
                     KKHKINLIDTPGYGIFSSEMICAMRAADAALIVVDAVAGVEVQTEKAWQVADDFGLPR
                     MIVVNRMDRENADGGRVMDQLQKKFGRACVCIEAPIGREKSFRGAIDLIGMKGFLTDD
                     AGKTAEGPIPDEFAADAATLHEALVEMVAEGDDSLMETFFSQGNLSEEQMLPALRKAI
                     HDRRIVPVLFAAAGDHERGVEKLLDEIVDLLPGADSFPAAATGKDGQTDVLPCDPNGP
                     LAAIVFKTISDPFTGRLTLFRVHSGTVKADGSYWNTNRETAERFGPVFSPLGKTMNTV
                     PEVVAGDIGVVAKLKETLTGDTLSAKEKPYRFPAVAIPEPAISFSIEPKSKGDEDKIS
                     LALSKLTEEDPSLKFHRDPDTSELLLSGSGQLHVEIAVARMKRKSNVEVILHPPKVPY
                     RETITKPAEAHGRHKKQTGGHGQFADCKIRVKPLPRGSDFEFVDDIFGGSIPRNYIPA
                     VEKGIQDTRRRGYLAGYPMVDFRVELYDGQYHDVDSSEMAFKIAGSLAFKDAMEKAKP
                     TILEPIMKVGVRAPQEYMGDLMGDLTSRRGRPSGMDQIGDDAVVKAEVPLSEMLSYSA
                     VLRSLTQGRGSFTMELSHYEELPKPLQEKLIAERAKLRKAEVETE"
BASE COUNT         2065 a         4636 c         4582 g         2007 t
ORIGIN      
        1 cggccgttcg tgacgcacca caacgcgctc gacgtcgacc tctacctgag gatcgccccc
       61 gagctctacc tgaagcggct gatcacgggc gggttcccga aggtctacga gatcaaccgc
      121 aacttccgca acgaggggat ctccacccag cacaaccccg agttcacgat gctggagttc
      181 tacaccgcgt acgccgacgc ccgcgaccag atggccctca ccgaggagct cctggccggg
      241 gcggcccggg agatcctcgg gacgacgcag ctcccgtggg gcgacgagac gatctccttc
      301 gaggcgccgt tccgccggct ctcgatgaag gacgccgtcg tcgagtacgg ccgggacgtc
      361 ccgggcgggg cgatccgggc agacgagctt gcgacgctcg aagggctgaa gggggcggcg
      421 gagcgggcgg gggtcgagtc gatcgagcgc ttcggcgacg tccccgggaa gctcctggcc
      481 gagctcttcg agacgctcgc cgagccgaac ctcctccagc cgacgttcgt tgtcgactac
      541 ccggccgaga tctccccgct cgccaagacc cgcccggacg acccgcggac ggccgaccgc
      601 ttcgagctct tcgtcggccg gatggagctg gcgaacggct tctcggagct gaacgacccc
      661 gacgagcagg cggagaagtt ccgtgcgcag gtggccgagc ggacgaaggg cgacgacgag
      721 gcgatgctct tcgacgagga ctacgtcgag gcgctctcgt acggaatgcc cccgacggcg
      781 ggcgagggag tcgggatcga ccgcctcgcg atgctcttca cgaactcgcg ctcgatccgc
      841 gacgtgatcc tcttccctct catgcgaccg aaggcggcgg gccgctgacg gaagccctgc
      901 tcgcccgccg atacctcctc gcctcgaggc gcgacgcgca ggtgggcgtc gtcgcgctcg
      961 ccgccttcgt cgggctcgtc ctcggcgtcg cggccctcgt cggcgcgctc gcgctcctct
     1021 ccggcttcca gtcgcacgtc ctctcgaggc tcgccgacga gacaccgcac gtcctcgtca
     1081 ccccggccgg ccgtcccgac ttcgcgggag aggagggggc ggggcggacg ctcgccgcgc
     1141 tgccgggcgt cgtctccgtc gtgcccgtcg tgcgggggcg cggctggatc acggcgcgcg
     1201 gccaggcggt ccccgccgcc ctcaccggtc gcgaaggcgc ggacgggatc acgctcgacc
     1261 cttcccagtc ccggcagctc tcggcgtttc cgggcgagga ggtggtcgtc gtgtcgtcgc
     1321 gctcgcggct ctctccgatc ggcccggtcc cgatcaccgc ctcgctcccg gtgacggacg
     1381 tggcggtcgc gtcgacggga cggcatcagc ccgaggcggt ccttcccgcc gacgcggccc
     1441 ggcggctctt cggcctcccg tcggccggcg ccaccggata cgagcttcgg ctcgccgacg
     1501 ccgacgccgc ggccgacgtc gcggcccgcg cccgcgcggc cctcggcgag acggccacgg
     1561 cgacgacgac gtggcaggag gcgaaccggc cgctcctcct cgcgctgaag ctcgagaggc
     1621 tggcgatttt cgccacggtc ttcctcgtcg tcgtcgtcgc cggactgaac ctcgccgcca
     1681 cgtcggccgt cctcgcggcg acgcgccggg gcgacgccgc ggtcctcacc gtgctcgggg
     1741 cggggcccgc cctcctcgcg cgcgtcttcc tcctcgccgg cctcctcctc ggcgcggccg
     1801 ggacgctctg cggcctcgcc gtcggcgcga tcctcgcgat cgtccttgat gccacgaagg
     1861 cgcttccgct ccccgcgcag ctcttctccc tcgcgcacgt gccgttccgg gtgaccgcgc
     1921 gggacctcct cgccgtcggg acgttctccc tcctctggtc gttcgtcgcg tcgctcgtcc
     1981 cggcgcggat ggcggcccgc gtcgacgtgg cggaggcgct ccgtgccggc tgagccgatt
     2041 ctccgggccg agtcgctcgc gaagggcttc ggcgaggcgg aggcccgcgt cgagctcttc
     2101 tccggcctct cgctggagct gggcgcggga gaggcgacgg cgatcgtcgg cccgtcggga
     2161 tgcgggaagt cgacgctcct ccacctcctc gcgggcctcg accgcccgga cgcggggcgc
     2221 gtcctcgtcg acggacgcga ctggaccgcg ctggcgccgg agccgcgggc gaggtggcgc
     2281 ggcgaggccg tggggttcgt cttccagctc caccacctcc tccccgagct gaccgccgaa
     2341 gagaacgtcg cgctgccgct cctcctcgcc ggctcgccgg aaggcgcggc gcgcgcggcg
     2401 gcccgcgagc tcctctcccg ggtcggcctc tcccggcagt ccggcgcctt tccccgggtc
     2461 ctctcgggag gcgagcgcca gcgggtcgcg atcgcccggg ccgtcgtgcg cagcccccgg
     2521 ctgctcctgt gcgacgagcc gaccggcagc ctcgacgccg cccacgctgc cgacgtcctg
     2581 gagctcctcc tcggcgcggc gcgcgagcgg gcgggcgcgc tcctcctcgt cacgcacgat
     2641 cccggcgtgg cgtcgcggtg cggatcggtg cggacgctga cgccggacgg cctctccgcc
     2701 gcctgagcgg cacccgaagg ctggcacccc ctgtgctagg atcgggagac ggtcgaagcc
     2761 cgctttccga gcggcctcgt cgaggtcgag ccatgttcga gaagtacaac gagaaagcgc
     2821 gccgcgccct cttcttcgcg cgatacgagg cctcgaagct cggctcgaag gtgatcgagt
     2881 cggagcacgt cctcctcggg atcctccggg agggggagga cgtcacccgc gagatcttcg
     2941 cccgcttcaa cgtgaagccc gacgacgtcc ggaaggagat cgaaggggac cgggtcttcg
     3001 tcgagcgcgt ctcctcgacc caggagctcc cgctctcgga ggagtcgaag aagatccttg
     3061 cctacgcttc ccacgaggcc gagtcgatga tgcatccgta cgtcgggacg gagcacctcc
     3121 tcatcgggat cctccgggtc gacccgtgcg tcgcctcccg cctcctgacc gcgcacggct
     3181 tcaacctcta cggcgtgcgg gaggaggcga tcgccctcat caaggagcgc gaagcctcca
     3241 agcagaagaa ggagctcccg ttcctcgccg agtacgcgcg cgacctgacg gccctcgcgg
     3301 cgcaggggac gttcgacccg ctcgtcggcc gcgacaagga ggtcgagcgg atcatccaga
     3361 tcctctcgcg acggacgaag aacaacccga tcctcctcgg ggagccgggc gtcggcaaga
     3421 ccgcgatcgt cgaaggcctc gcccagcgga tcgtcgaagg cgcggtgccg ctcttcctcg
     3481 cgaaccggaa gatcctcgcg atggacctct ccctcatcgt ggcggggacg aagtaccgcg
     3541 gtcagttcga ggaaaggctc aaggggatcc tgaaggagct ccgggagaac caggacctca
     3601 tcatcttcat cgacgagatc cactcgctga tcggggcggg ctcggccgaa ggctcgctcg
     3661 acgcggcgaa catcctgaag ccggccctct cccggggcga gatcgcctgc atcggcgcga
     3721 cgaccctcaa ggagtaccgc aagtacatcg agaaggaccg ctcgctcctc cggcgcttcc
     3781 aggcaatcac cgtgaacccg ccgagcgagg aggagactct cgagatcctc gagggggtcc
     3841 gcgagcgcta cgagaggttc caccgggtgc ggtacgcccc cgacgcggtg aagacggccg
     3901 tctaccagtc gagccgctac attcccgacc gcttcttccc cgacaaggcg atcgacctcc
     3961 tcgacgaggc gggcgcgaag gtgaagctcc gcatgacggc ggacacgcag aacctcaggc
     4021 gcctcgagaa cgaggtgaac cagatcgtca aggagatgaa gaaggcgatc tccgacaagg
     4081 acttcgagca cgccgtctcg ctccgcgagc gcgaggtcga gatcaaggag gagatcgagc
     4141 ggacccggaa cgcccagctc gacgaagggg cctgggccga ggtcgtccgg ggcgacatcg
     4201 aggagatcat cgcgagctgg accggcatcc cggtcacctc gatccaggtc gaggaagccg
     4261 agaagctcct gaacatggag acgacgctcc ggaagagggt catcggacag gaccgggcga
     4321 tcgacgccat ctcccgcgcg atccgtcgct cgcggctcgg cgtgaagaac ccgaaccggc
     4381 cgatgggctc cttcatcttc ctcggccctt cgggcgtcgg gaagaccgag gtcgcccggc
     4441 gcctcgccga gttcctgttc gacaaccaga aggcgctcgt ccgcttcgac atgtccgagt
     4501 acatggagaa gcacgcggtc tccaagctga tcggctctcc gcccgggtac gtcgggcacg
     4561 aggagggggg gcagctcacg gagcgaatcc ggaggcagcc ttactcggtc atcctcctcg
     4621 acgagatcga gaaggcgcat cccgacatcg cgaacctgct cctccagatc ctggaggacg
     4681 ggcagctgac cgacgcctac gggaacacgg tcgacttcaa gaacacgctc atcatcatga
     4741 cctcgaacat cgggacgaag tacctcgtca cccaggcgcg cgtcggcttc ggcgacggga
     4801 agaacgcgcc gcccgcccgc gaggtcgagg agatggtcct gaaggagctc cgccgcgagt
     4861 tctcgccgga gttcacgaac cggatcgacg aggtcatcgt cttcaacccg ctcacgacgg
     4921 agaacctccg ggccatctgc cggctccttc tcgacgacgt cgcccagacc ctgaagcacc
     4981 ggggcctcga gctcgtcgcc gacgagaagg cggttgactg gctgctctcg atgtcggggc
     5041 aggacgccca ctcgggcgcc cggccgctcc gaaggacgat ccaacgccac gtggaggatg
     5101 cggtatccga attgcttatc ggcgcccggg agcagctcga gtgcctcgaa gtcaccgtga
     5161 tcgaaagcga actgaaggtt ctcgtccgct cggcggccgc gctggcgacc gagcggtaag
     5221 cgacgtgcag gacgtgtaca tcgaccctga tcggggtgcg ggcgcacccg ggtggccgct
     5281 tggcgcccgg cgtcccgcgg gccgtgtccg ggtcgccgcg atcctggccc tcttcctcgg
     5341 cggtgcggtc ccggccccgg cacagacgca gggggagccg cccgccgctc cggcggcgcc
     5401 tcccgccact ctcgacctga gaccgggggc cgcggggccg caggctccgc cttctccgct
     5461 cgccggggcc cttccgcccg ctccggcgct ccgaacgtcc ccgggggccg acgggaggga
     5521 gcggatcgcc gagatcgccg tccgcggcgg ccggaccgtc accgcggaga cgatcgcgtt
     5581 ctacctcggg gtgaaggtcg gcgacccgta cgacacggag gccctgcggc gtgccttccc
     5641 gaagctctgg gaatcgggcc tcttcgagga cctccgtctc gagcgggaga gcgttccggg
     5701 cgggtcccgg atcgtcgcgg tcgtcgtcga gcgcccccgg atcggcgatc tcgagttccg
     5761 cgggaacaag aagctgacga cctcccagct gaaggacaag ctgaaggagg ggaaggtgga
     5821 gatccgcgtg ggatcgccgc tctccctcag cgacgtctcc aaggggaagg gcgtcctcat
     5881 ggaggcctac caggccgacg gattccggtc cgcggtcgta gacacggccc tcgagccgat
     5941 gggggagaac gagaagcgcg tcgtcttcct cgtcgacgag ggagacaaga tcaagatcga
     6001 ggcgatcgac ttcaccggga acacggtctt ctccgaccgg cgcctccggt tcgcgatgaa
     6061 gaagacccgg cagcggaact tctaccagtt ctgggactcg aaggacgtct acaaccaggc
     6121 gaactacgag gaggacatcg agtccgtcaa gaaggtctac cagaacgccg gatacaagga
     6181 catcgtcgcg aaggacccgg tgatcgagac cttcgtcgtg aaccccgacg agacccgccg
     6241 ggacaagatc aagcggcggg cccggatcga gatcccgctc gtcgaggggg agcgcttcta
     6301 cttcggcgac gtgaaggtcg aggggacgac cgtcttcccc ggcgaccggc tcctccagtt
     6361 cttcgaatgg agccccggca agcccctcaa gaggtcggcg ctcgtcgacg gcatgaaggc
     6421 gctcgacgag atctaccgcg ggcggggcta catctacgcc ttcatgaacc cggagtacac
     6481 cgagcggccg ggtgagggga acctcgtcga cgtcgtcgtc cgggtcaccg agggcgacat
     6541 gttccgcgtc ggccgtgtcg agttcaccgg gaacaagacg acgaaggaca aggtcctccg
     6601 gcgggagatc cccctgttcg agggcgacgt cctcgacatg gagtcgttca agaaggggct
     6661 cctgaagctc tcccagctcg gctacttcaa gatcgaggaa gaccccgact tccgcgtcga
     6721 cccggagaag aaggtcgtcg acgtgacgat caagggcctc gacacgaacc ggaacgagat
     6781 ccagttcggc gccgggtact cgcagctcga cggcgtgttc ggacagttcc agttcgcgac
     6841 ccggaacttc ctcggacgcg gcgacacgat cagcgtccag ttccagcggg gcggctactc
     6901 gaacttcttc gacctctcct tcgtggagcc ctggttcctc gacaagcgga tgtcggtcgg
     6961 cggctcggtc ttcaaccgct cgctcgagta cctcgacgtc gaccagcaga cgaagggcgt
     7021 caacatgtcc ttcgggctcg gactcggcct gttcgacgga ctgtcgcttt tctacggcta
     7081 caccgacacg aagagccgct accaggtcta cccggccgct cctcccccgg gctcgggcat
     7141 cccgcccgcc gcctacgccg actacatcgg gaagacctcc gcgatcgcgc ccggctaccg
     7201 gtacgacagc cggaacgacc cgttcgaccc gtcccgcgga cgacggttca acttctcgac
     7261 gacgatcgcg ggcgggccgc tcggcgggaa cttctcgtac atcaagccag tcggcggggc
     7321 gacgtcctat ttccggacgg gccggaagtc ccatttcgcc gtcaacatgg agggcgggtt
     7381 catccgcgcc tacgacggca aggagatccc gatcttcgag cgtttccgaa tcggaggcga
     7441 tcgctcggtg cgcggcttcc agtacgggag catctacccg ctcgacgacg acgacaaggc
     7501 gttcttcacc gatcagggcg cgctcctggg cggagacaag tacttcgtct tcaacgccga
     7561 gctggtccac gctatcgcgg ggccgctgaa gctcgtcctc ttcttcgacg ccgggaacgc
     7621 ctggctggag gggcagtcct tcaacccgct caaggcgcgc gcctcgacgg gcgccgagct
     7681 ccggatgttc ctcccgatct tccaggcgcc gctgcgcttc atctacggga tcaacctcga
     7741 tcccaagacc atcaaggggc ctgacggatc gccgctcacg ggcggcgcgg agaagagcag
     7801 cgacttccag ttctccatcg ggacgacctt ctagaggggg aacccggacc gccgggttcc
     7861 ccctcacccc ctccgcgcgg agaggcagct cgctgtcgct cgcacggagt caccggggga
     7921 acccggaccg ccgggttggc ttcgccttcg aaccgggtgc gcgtggggcg cacccggctc
     7981 ggtccccctc gaaccccctc cgcgcggaga gggcgcccgt tgagcgcgga ggcggcgaag
     8041 ggatcccttc ccgcacgccg gcggagtcgg ttggtgcagt agaatcgcgc gccccgctgg
     8101 aaagcctccc gcggggccag acacggaaga aggagtcacg tagatgatcg ggaaccggat
     8161 gttcgtcgcg ggcgtcgtcg cgacggccgc actcgtcgct ctccccgctt cggcccagtc
     8221 cgcccagaag gtcggtgtca tcgaggtcca gcggatcgtc caggactccg cggtgggcaa
     8281 ggagagcctc gcccggatcc agaaggtctc gacggccaag caagaggacc tgacgaagcg
     8341 ccagaccgag ctccgcgacc tcgagaagcg gatccaggag cagggcaagt cgctctccga
     8401 agaggcgatg gagaagctcc agaaggacta ccagcagaag gctctcgacc tcaagcgctt
     8461 ccaggacgac gcgcagcggg agctcgagga gctccagcgc cgcgagctga cggatctcga
     8521 gaagaaggtc cttcccgtca tcgaggcggt ctccaaggag ctcgggtaca cgctcgtctt
     8581 caacaagttc cagagcgggc tcctctacgc tgacgacgcg gtcgacctca ccgcggccgt
     8641 catccagaag ttcaacacgg cgatcgcggc cgctccgaag accgacgcca agccggctgc
     8701 cgcgacgcca gcgccgaaga agtaagaggg ggatggcccg cttccccgcg ccgacgaccg
     8761 ccgccgcggt ggcttccgcc tgcggcgggc ggctcgtcgg cgacgccggg accgtcgtcg
     8821 agggggcgcg gtcgctcgag tcggcgggac ccgccgacct cgcgttcgcc gccgacgcga
     8881 aggcggagca ggcggcggcc acatcggcgg ccggggttct cctggcgcga tcggcgctcg
     8941 ggttcccggg ccgagcggtc gtcgaggtgc cggaccccgc gctcgcattc gccggcgtcc
     9001 tcgaggcgtt cttttcgcgc cgagcgccgg ccccggggat ccacgcgacc gcgatcgtcg
     9061 aagccggcgc ccgcgtcgcg gaagacgcca cgatcggacc gtacgcggtc gtgggggcgg
     9121 agacggagat cggaagtggc gccgtcctcg gggcgcacgt cgtcctgggc cggcggtgcc
     9181 gggtcggcgc gggcgcgtgg ctgcaccctc acgtcgtcct ttatgacgac gtctcgatcg
     9241 gcccgggctc ggaaatccac gcgggggcgg tcctcggagc cgacggattc ggctacgtcg
     9301 cgtctccgac gggactgaag aaggtacctc aggtcggcgg cgtcgaggtc ggttcctccg
     9361 tcgagatcgg cgcgaacacg tgcgtcgacc gggcgacgct cgaggcgacg cgcgtcggcg
     9421 acgggacgaa gatcgacgac ctcgtcatgg tcgggcacaa ctgcgaggtc ggggcccacg
     9481 tcatcctctg cggacaggtg gggctcgccg ggtcgacgac cgtgggagat caggccgttc
     9541 tcggggggca ggtgggaacc gccggccacc tccggatcgg ccgcggggtg aaggcgggag
     9601 gccagacggg gatcgtcgga gacgttcccg acggcgcctc ccttttcggt acgccgcatc
     9661 tgccgcaccg ggacgccttt cggatccatg ccgagctgaa gaagctcccc gagaccgcgc
     9721 gtctcgtacg cgagctcgcc cgggcgggcg aggagaagag atgagcgaag cgccgacgga
     9781 ccggggcggt ctcgacatcc gcgcgatcct ccggacgctc ccgcatcgct atccgttcct
     9841 cctggtggac cggatcctgg agatcgtccc ggggcagaag gtcgtcgggc tcaagaacgt
     9901 cacgatcaac gaggagttct tccaggggca tttccccggg aacccggtga tgccgggagt
     9961 cctcctcgtc gaggcgatgg cgcaggtcgg cggcttcatg atgctcgccg aggtcgacga
    10021 cccccagagc cggcaggtcc ggctgacggg cctcgacgcg gtccggttcc ggcgacccgt
    10081 cgttccgggc gaccaggtcg tcttcaccgt ggagttcgtg gcccggaaga agtcgctcgt
    10141 gaaagtgcgc ggggaagccc gggtcgacgg ccagctctgc gtcgaggcgg agatcatgag
    10201 cctgatccag gactgcgggc gggaagaggg agactgaatg ccgatcgacc gcacggcgat
    10261 cgtccatgag agcgcgaccg ttcatccgga cgccgtgatc ggcccatacg ccgtcgtcgg
    10321 cgcctccgtc gtcatcggcg cccggacccg gatcggtgcc cacgccgtcg tcgacggacc
    10381 gacggtcctg ggcgaggaca acgtcgtcca tccgcacgcc gcgctcggag gtccgcctca
    10441 ggacctgaag taccgcgacg agccgacccg gctcgtcgtc ggaagccgga acgtcttccg
    10501 ggagttcatg acggcccaca gggggaccgt caccgggcac cacgagaccg tcatcggcga
    10561 cgacaatctc ttcatggcgt actcccacgt cgcgcacgac tgcatcatcg gcagccggac
    10621 ggtcttcgcg aactcggctt cgctctcggg acacgtcacg gtcgaggacg acgtgatcct
    10681 cggcggattc gtcgtcgtga agcagttcct ccggatcggc cggttcgcct acgtgggcgg
    10741 catgacgccg gtcaacaagg acgtcctccc gttcacctgg acctccagcg accgcgacac
    10801 gaaggcctac aagatcaacg gcgtcggcct gtcgcggaag ggctactcgg ccgagcgggt
    10861 cgcggcgctc aacaaggcct accggatcct ctaccggaac cgccacgacc acgcggccgc
    10921 cctcgaggcc ctcgccgcgc tggccgcgac cgctcccgac gtgcaggtcc tcctcgactt
    10981 catctcgtcc tcgaaggcgg gggtccacgg ggcgtgattg gacgggggag acccggaccg
    11041 ccgggtctcc cccgtacccc ctcccgcgcg gagaggaagc tcacttcgtt cgcgcgggac
    11101 gaagccggac ccggaccgcc gggtctcccc cgtaccccct cccgcgcgga gaggaagctc
    11161 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcgttcg cgcgggacga
    11281 ggctggaccc ggaccgctgg gtcacagcga catcggcatc aaggcgaagg ggccgctcgc
    11341 gcggcccctt cgggttctcg ggtgggggag ctccggacct actcggtttc gacctcggcc
    11401 ttgcggagct tcgcgcgctc ggcgatgagc ttctcctgga gcggcttcgg cagctcctcg
    11461 tagtgggaaa gctccatcgt gaacgagccg cggccctggg tgagggaccg aaggacggcg
    11521 gagtaggaga gcatctcgga gagcgggacc tcggccttca cgacggcgtc gtcgccgatc
    11581 tggtccatgc cggaggggcg gccgcgccgg ctcgtgaggt ctcccatcag gtcgcccatg
    11641 tactcctgcg gggcccggac gccgaccttc atgatcggct cgaggatcgt cggcttcgcc
    11701 ttctccatgg cgtccttgaa ggcgagcgag ccggcgatct tgaacgccat ctccgacgag
    11761 tcgacgtcgt ggtactgacc gtcgtagagc tcgacgcgga agtcgaccat cgggtagccg
    11821 gcgaggtagc cgcggcgccg ggtgtcctgg atccccttct cgacggccgg gatgtagttg
    11881 cgggggatgg agccgccgaa gatgtcgtcg acgaactcga agtcgctccc gcgcgggagc
    11941 ggcttgaccc ggatcttgca gtcggcgaac tgcccgtggc cgccggtctg cttcttgtgg
    12001 cgtccgtggg cctcggccgg cttcgtgatc gtctcgcgat aggggacctt cggcgggtgg
    12061 aggatgacct cgacgttcga cttccgcttc atccgggcca cggcgatctc gacgtggagc
    12121 tggccggatc cggagagcag cagctcgctc gtgtcggggt cgcggtggaa cttgagcgac
    12181 gggtcttctt cggtgagctt cgagagcgcg agcgagatct tgtcctcgtc gcccttcgac
    12241 ttcggctcga tggagaagga gatcgccggc tcggggatgg cgacggcggg gaagcggtac
    12301 ggcttctcct tcgccgagag cgtgtcgccc gtcagcgtct ccttcagctt cgcgacgacg
    12361 ccgatgtcgc cggccacgac ctcgggcacc gtgttcatcg tcttgccgag cggggagaag
    12421 acgggaccga agcgctcggc cgtctcccgg ttcgtgttcc agtagctccc gtcggccttg
    12481 accgtcccgg aatggacgcg gaagagagtc agccggccgg tgaacgggtc ggagatcgtc
    12541 ttgaagacga tcgcggcgag cgggccgttc gggtcgcacg gcaggacgtc cgtctggccg
    12601 tccttgccgg tggcggcggc cgggaaggag tcggcgccgg gaaggaggtc gacgatctcg
    12661 tcgaggagct tctcgacgcc gcgctcgtgg tcgccggcgg cggcgaagag caccgggacg
    12721 atccgacggt cgtggatcgc cttccgcagc gccgggagca tctgctcctc ggagaggttc
    12781 ccctgcgaga agaaggtctc catgagggag tcgtccccct cggccaccat ctcgacgagc
    12841 gcctcgtgga gcgtggcggc gtcggcggcg aactcgtccg ggatcgggcc ttcggccgtc
    12901 ttgccggcgt cgtccgtcag gaagcccttc atcccgatca ggtcgatcgc cccgcggaac
    12961 gacttctccc ggccgatcgg cgcctcgatg cagacgcagg cccggccgaa cttcttctgg
    13021 agctggtcca tcacccggcc gccgtcggcg ttctcgcggt ccatccggtt gacgacgatc
    13081 atccggggaa ggccgaagtc gtcggcgacc tgccaggcct tctcggtctg gacctcgacg
    13141 ccggcgacgg catcgacgac gatgagggcc gcgtctgcgg cgcgcatcgc gcagatcatc
    13201 tcggacgaga agatcccgta gcccggagtg tcgatcaggt tgatcttgtg cttcttccac
    13261 tccagtgcgg ccacggccag cccgatcgag atcttcctct cgatctcctc cgggtcgaaa
    13321 tcggtcggcg ccgaaccgtc gtcgacgcgg ccgaagcggt tcaccgctcc cgcctcgtga
    13381 aggagcgccg
//
LOCUS       DAOONL010000089        13359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5088_length_13359_cov_16.633870, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000089 DAOONL010000000
VERSION     DAOONL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_13359_cov_16.633870"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLP50_12440"
     CDS             <1..236
                     /locus_tag="PLP50_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD/FAD-binding protein"
                     /protein_id="HPA52397.1"
                     /translation="IVSLNPRREPAEGTVARRLLYTHPVYTPASVATQAELPSLNGKR
                     RTWFAGAWFGYGFHEDGLRSGLEAAVSLGAEPL"
     gene            233..1030
                     /locus_tag="PLP50_12445"
     CDS             233..1030
                     /locus_tag="PLP50_12445"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HPA52398.1"
                     /translation="MTGRETKRPLASALYVGTVRHRRLTPKSHAFSYRVWQLLLDLDE
                     LPRIEEEVGLFRHNRSGWTALFDRDYLGPAERPVKEKLRDWLAARGHVLGERRVLLLT
                     HARVLGYAFNPVSYYYVLGADGRLDLAVAEINNTFGETFGYVLERRGDEPGIRTPRFP
                     KVFHVSPFLPMDLEYEFHLSVPGEGLVVHVDDFESGVKVFDATFSARRRPLTTASLAR
                     ALLANPLMPAMVIAWIHWQALKLWLRELPVYTRPEPPAGLIHTRRAA"
     gene            1027..2316
                     /locus_tag="PLP50_12450"
     CDS             1027..2316
                     /locus_tag="PLP50_12450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPA52399.1"
                     /translation="MTTLESTSDALVSGAPSPGLLDRLAARLALRALEGIREGAVLLT
                     LPDGTTRRFGAEEARPVRLVARSWRPFRALLAAGDLGAAEAYLDGEWTADDLPGLVRL
                     FARNADLLDRETWATRLTNVANRLLHARNRNSRAGSRRNVPAHYDLGNDLYETFLDPS
                     MTYSCALFETGDETLEEAQEKKLRRIAEKARIRPGDHVLEIGCGWGAFALLAAREYGA
                     RVTGITLSTEQAARARERVAREGLADRVEIRLVDYRDLPAEGRTYDRVVSVEMLEAVG
                     EEFLPGYFEVVDRLLAPDGIAVIQSITVPDDRYARYRRRPDFIQKYVFPGSHCPSVGA
                     IVSAVSARSRLLVHHLEDVGAHYAETLRRWREAFLANRPRVRQLGYDERFTRLWDFYL
                     AYCEGGFAERHIGDVQVVLTRSGNHALGPAPGYRGEA"
     gene            2313..2870
                     /locus_tag="PLP50_12455"
     CDS             2313..2870
                     /locus_tag="PLP50_12455"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPA52400.1"
                     /translation="MIEVLRRRLVRPILEQLTQGLSPDAIALTIAIGLAIAVIPIVGT
                     TTILCTTAAIVLRLNQPLIQAINYLSFPLQLAFILPYLKLGRLLFGGPAVRMSAEELA
                     AFVTSRPGEAIEALWRVTLQAVGAWALTAPLIGAAVYFAVRPALRAAARRLRPTKGAD
                     APTGPAAGAELATVPVPAAEESDAR"
     gene            2860..3915
                     /locus_tag="PLP50_12460"
     CDS             2860..3915
                     /locus_tag="PLP50_12460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HPA52401.1"
                     /translation="MPVEHGWAQLALERGLVPDVLVRRRIRELLRRRLAEERAGDAEA
                     ALEREERLVAQMRESPVALATAEANEQHYEVPPEFFELVLGAYRKYSSGLWSDGVTTL
                     DAAEAAMLALTCARARLEDGQNVLELGCGWGSLTLWMAERYPASRIVAVSNSRSQREY
                     VEARAAERNLSNVKVVTCDMNVFDPATGPAFDRVVSVEMFEHMRNWPELLGRIASWLK
                     PDGRLFVHVFSHRDVSYPFVARDESDWMARSFFTGGLMPSDHLLLRFPEHLFVEERWR
                     VNGTHYARTAEAWLENLDRRRAGARKLFEKAYGPALARRKLAEWRVFLMACAELWAFG
                     GGNEWMVSHYRLRRRYV"
     gene            3974..4624
                     /gene="msrA"
                     /locus_tag="PLP50_12465"
     CDS             3974..4624
                     /gene="msrA"
                     /locus_tag="PLP50_12465"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPA52402.1"
                     /translation="MFDKSKLRLPDSEDALEGRPTPLAVTNRHFVNGREILPPFPAGL
                     ERAVFGMGCFWGAEKAFWALPGVHTTAVGYSGGLTPNPTYKEVCTGRTGHAEVVLVVF
                     DPAAVSYEALLKLFFEKHDPTQGMRQGNDVGTQYRSLVLTHSAEQDRLATAIRDVYQA
                     VLSARGFDPITTGIRPAGPFYYAEDAHQQYLAKNPGGYCGLGGIGIACPVGLGVDG"
     gene            complement(4644..6989)
                     /locus_tag="PLP50_12470"
     CDS             complement(4644..6989)
                     /locus_tag="PLP50_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPA52403.1"
                     /translation="MTKERITSFLEKAVFGHRPVVLGLFAAATLFMAWSAAHLKVDAG
                     FQKNLPHAHPYMETFLKHQQEFGGANRVLVAMMAREGDIFTPEFLDALKKATDEVFFI
                     PGVDRSRVQSLFTPNVRYTEVVEDGIAAGNVIPDDFEPDAEGLAQVRRNVLKAGIVGR
                     LVANDFSGTIISAELLEVDPSTGQKLDFIAVSNALEEKVRRQFDAEFVPSSKVDVRII
                     GFAKVVGAISAGTRRVILFFLVAYALTGLLVYLYSQSHRLSLVALGCSTIAVVWQLGL
                     LTLLGFGIDPMSILVPFLIFAIGVSHAVQMISANGAEIFDGADPMTAARNSFRRLLVP
                     GFIALASDTIGFLTILLIKIRVIQEIAITASLGVAVIILTNLFLIPVVLSYLHYPADY
                     NARLHRRAEHMSGLWRRLAQVSAPKPAAVIVAVAALLFGLGWWKGRDVKVGDLHRGVP
                     ELRADSRYNVDSAVITSKFSIGVDILTAIVETKPEGCVDHSVMTTIDDFAWHMENVEG
                     VQSTIALPAIAKMLNAGWNEGSPKWRVLSRNQSVLQQSVTYVPTTSGLLNSDCSVMPV
                     MMFTADHKADTIERIVAEVKRFEADHGHPDVKLRLATGNVGVMAATNEAVSAAQFPIL
                     AWVFGAIVVLCLLSFRSLRGTLCILVPLALVSLLAYALMAWLKIGLKVSTLPVVALGV
                     GVGVDYGIYIFARFRSLYRDGVPLTEAYRKTLAITGNGVLFTGITLGLGVATWIFSPL
                     KFQADMGLLLCFLFLMNMVGALVLLPALARGLLGDTHQGRG"
     gene            complement(7045..8004)
                     /locus_tag="PLP50_12475"
     CDS             complement(7045..8004)
                     /locus_tag="PLP50_12475"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPA52404.1"
                     /translation="MREKSVPALGAALLALATLAPAGAAEPPAASERSTPARLAARSL
                     LLDAADRDGLAVAVGERGHVLVSRDGGASWTQAEAPTRALLTGVFLHDSRLGWAVGHD
                     ETILRTRDGGATWQRVRHAPEEERPLLDVWFRDERQGFAIGAYGLLLATGDGGDTWES
                     RTVVAGDDTHLNAIAEADGALILAGEAGSLYRSDDGGESWKALPSPYEGSFFGLLSLS
                     DGGLLAFGLRGHLFRSEDRGTTWTGVASGTEATLMTGRELGGGRVVVAGLAGVLLFSE
                     DAGRTFRAREQEDRKASLAILPGGAGGIVLLGEGGVRPIEKPF"
     gene            complement(8021..9382)
                     /locus_tag="PLP50_12480"
     CDS             complement(8021..9382)
                     /locus_tag="PLP50_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HPA52405.1"
                     /translation="MRRNTRILLCTLTLSALLGGSALAQLSPQDAAKLGASLTPLGGE
                     KAGNADGSIPAWDGGLTKAPAGWKPGTHYADPFAGEKPLFTIDGKNAAQHAAKLSEGH
                     KAMLKAYPSFRMPVYPTHRTGAAPQRIYDATKKVATTAKLAEGGNGVTGAVGGIPFPI
                     PKSGIEVIWNHLLRYRAEIAGRTVAQAAPTRGGQYTLVQFEEETMFVYHLPGTTEASM
                     KNRLLNFKQAVTAPARLAGGILLVHESMNQVAQPRDAWLYNPGQRRVRRAPQVAYDNP
                     GTAADNMRTSDQLDMFNGAPDKYDWTLVGKKEIYVPYNAYRLQDPKVKYKDILTPLHM
                     NPDHTRYELHRVWVVDATLKKGERHIYKRRTFYIDEDSWQILVVDQYDNRDQLWRVSE
                     GHAIAYYDLPTIWTSAESHTDLQAGRYLVMGLNSENAPHDFKIKRSEGDFMPDALRRE
                     GVR"
     gene            complement(9399..11204)
                     /locus_tag="PLP50_12485"
     CDS             complement(9399..11204)
                     /locus_tag="PLP50_12485"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HPA52406.1"
                     /translation="MTTRSAAIAALAAAFLAPLARTAPAIDGKQGDFSWSWDTTVSYG
                     LFARVEKRDRALVGLASGGTAFSVNGDDGNLNYDTGLASSAFKATTELELTYKRIGAF
                     VRAYGFYDIENEERDRDRTPLSPEALDRVGSRAEIRDAFLWAKFDLGPVPGEIRAGWQ
                     VINWGESTFIQGGINAINPVDVSALRVPGSELRDALLPVGAVLLSVKPSKNTSLEGFW
                     QYAWTQTKIDPVGSYFSTTDLAGAGATKVMLGFGSAPDSIPVGFNIPNNPVGVAVPRT
                     GDREADDSGQYGAAFRLFVPALGGTEFGLFYMNYHSRLPLIMARTGTQAGLLTRGNYA
                     ASASYFLSYPEDIKLYGLSFNAQLGSTGVALQGEVSHRKDVPLQVDDVEILYAALTPL
                     RLLPAAPQLAPLRQLGGLLAATNQAGAYGFDEEIRGYRLFDTTQAQLTATRVFGRVLG
                     ADQLVLVAEGAWSKVHDLPEQSVLRLEAPGTYTSGNPAHQQAGVQPGTEPSTAFPTSS
                     SWGYVVAGRLDYSNAIGAINLAPRFSFAHDVNGVSPGPGGSFIEDRMALTLGLGFQYR
                     INVEWDLSYTRYFGAGRYNLINDRDFLAGNVKYSF"
     gene            complement(11403..12857)
                     /locus_tag="PLP50_12490"
     CDS             complement(11403..12857)
                     /locus_tag="PLP50_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPA52407.1"
                     /translation="MRRTPQHFHGAAFGAALLLLAPLAAPGSGAAPALLDDFTPQTMR
                     VDLLHTGGKGAEIVVVDRVVNDGPWAGSLTKADDGLGLGSYRFEVLDPSTGRLLYARG
                     YSSIYAEWETTSEAKATHRTFRESLRFPWPLRPVQVALKKRDRQNLFREVWGTLVDPA
                     SPDVNPAPPPKVGTVWTVFESGPATEKVDLLVLGEGYAEKDLPKFRADVKRMVDTLFR
                     YEPFRSRKGDFNVRALDLASGDGGAHRPQSKLFRRTPLQVQYNVFGSERYVLTYDDRA
                     LRDAAAQAPYDVLEILVNDAQYGGGGIYNNQATASADTAFADYVFVHEFGHHFAALAD
                     EYYTSDVAYETGAADLPEPWEMNVTALKDPANPKWKELVEAGTPLPTPWSKDAFEKAS
                     REYQARRKALLAKGVPPSEIDALFREQQKAEAAFFAKERFAGKVGAFEGAAYEAKGLY
                     RSSADCIMFTRTTGGFCPVCRKAIEKVIDSYARP"
     gene            12903..>13359
                     /gene="thpR"
                     /locus_tag="PLP50_12495"
     CDS             12903..>13359
                     /gene="thpR"
                     /locus_tag="PLP50_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPA52408.1"
                     /translation="MIRTFVAVPVEDAVVRRRLAGARSLLPELRGVRWTAERQLHFTL
                     KFLGDLEAERLADARQATAAAAHRGPGSFRLALEGLGAFPPRGAARVVWAGCGSGATE
                     LASLAGTVEEAFEAAGFPREERPFSPHLTLARVRDPLSGRRLAQALPSVP"
BASE COUNT         1883 a         4561 c         4877 g         2038 t
ORIGIN      
        1 ggatcgtctc cctgaacccg cggcgcgagc cggcggaggg gacggtcgcg cggcgcctcc
       61 tctacaccca cccggtctac accccggcga gcgtcgcgac gcaggccgag ctcccgtcgc
      121 tgaacgggaa gcggcggacc tggttcgcgg gcgcctggtt cggctacggt ttccacgagg
      181 acgggctgcg gtcgggcctc gaggccgccg tctcgctcgg cgcggagccg ctgtgaccgg
      241 acgggagacg aagaggccgc ttgcctcggc cctctacgtc ggcacggtcc gccaccggcg
      301 gctcacgccg aagtcccacg ccttctcgta ccgggtgtgg cagctcctcc tcgacctcga
      361 cgagctgccc cggatcgagg aagaggtcgg cctcttccgg cacaaccgga gcggctggac
      421 cgcgctcttc gaccgcgact acctcgggcc ggcggagagg ccggtcaaag agaagctccg
      481 cgactggctc gccgcgcgcg gccacgtcct cggcgagcgg cgcgtcctcc tcctgaccca
      541 cgcccgcgtc ctcggctacg ccttcaaccc ggtgagctac tactacgtcc tcggggcgga
      601 cggacggctg gacctcgccg tcgccgagat caacaacacc ttcggcgaga cgttcgggta
      661 cgtcctcgag cggcgcggag acgagccggg aatccggact ccccgtttcc cgaaggtctt
      721 ccacgtctcg cccttcctcc cgatggacct cgagtacgag ttccacctct cggtcccggg
      781 agagggcctc gtcgtccacg tcgacgactt cgagagcggc gtcaaggtct tcgacgcgac
      841 gttctcggcg cggcggcggc cgctgacgac ggcgagcctg gcgcgggccc tcctcgcgaa
      901 cccgctgatg ccggcgatgg tgatcgcctg gatccactgg caggcgctga agctctggct
      961 cagggagctc cccgtctaca cccgacccga gcccccggcc gggctgatcc acacgaggag
     1021 agccgcgtga ccactctcga gtcgacttcc gacgccctcg tctccggggc cccttcgccg
     1081 gggctcctcg accgcctcgc cgcgcggctc gctcttcgcg cgctcgaggg gattcgggaa
     1141 ggggccgtcc tcctgacgct cccggacggg acgacgcgcc gcttcggagc ggaggaggcc
     1201 cgccccgttc gcctcgtcgc gcgctcctgg aggccgttcc gggcgctcct cgcggccggc
     1261 gacctcggcg ccgcggaggc ctacctcgac ggcgagtgga cggccgacga ccttccgggc
     1321 ctcgtgaggc tcttcgcgag gaacgccgac ctcctcgacc gcgagacctg ggcgacccgc
     1381 ctcacgaacg tggcgaaccg gctcctgcat gcccggaatc ggaacagccg ggccggctcg
     1441 cggcggaacg tgccggcgca ttacgacctc ggcaacgacc tctacgaaac cttcctcgac
     1501 ccctcgatga cgtactcctg cgctctcttc gagacggggg acgagactct cgaagaggca
     1561 caggagaaga agctccgcag gattgccgag aaggcccgga tccggcccgg cgaccacgtc
     1621 ctcgagatcg gctgcgggtg gggcgccttc gccctcctcg ccgcgcggga gtacggagcc
     1681 cgtgtcaccg ggatcaccct ctccacggag caggcggccc gggcgcggga gcgggtggcg
     1741 cgggaagggc tcgcggatcg cgtcgagatc cggctcgtcg actaccgcga cctcccggcc
     1801 gagggaagga cgtacgatcg ggtcgtgtcg gtcgagatgc tcgaggcggt gggggaggag
     1861 ttcctcccgg gctatttcga ggtcgtcgac cggctcctcg cgcccgacgg gatcgcggtg
     1921 atccagtcga tcaccgttcc cgacgaccga tacgcccgct accgccgacg ccccgacttc
     1981 atccagaagt acgtctttcc ggggagccac tgcccctccg tcggggcgat cgtttcggcc
     2041 gtctccgccc gttcccgcct cctcgtccac cacctcgagg acgtcggggc gcactacgcc
     2101 gagacgctgc ggcggtggcg ggaggcgttc ctcgcgaacc ggccgcgggt gcgccagctc
     2161 ggctacgacg agcgattcac gcgcctctgg gacttctacc tggcttactg cgagggaggc
     2221 ttcgccgagc gtcatatcgg ggacgtccag gtcgtcctga cccgctcggg aaaccacgcc
     2281 ctcggccccg cgcccggcta ccggggagag gcgtgatcga ggtcctgcgc cggcggctcg
     2341 tccgcccgat cctcgagcag ctgacgcagg ggctctcccc ggacgcgatc gccctgacga
     2401 tcgcgatcgg cctcgcgatc gccgtgatcc cgatcgtcgg gacgacgacg atcctctgta
     2461 cgacggcggc gatcgtcctc cggctcaacc agccgctcat ccaggcgatc aactacctct
     2521 cgttcccgct ccagctcgcg ttcatcctcc cgtacctgaa gctcggtcgg ctcctcttcg
     2581 gcggaccggc cgtccggatg tcggccgagg agctcgcggc cttcgtgacg tcgcggcccg
     2641 gggaggcgat cgaggcgctc tggcgcgtga cgctccaggc cgtcggcgcc tgggccctga
     2701 cggcccctct gatcggcgcg gcggtctact tcgcggtgcg ccccgccctg cgcgccgccg
     2761 cgcggcggct ccggccgacg aagggggcgg acgccccgac cggaccggcg gccggggcgg
     2821 agctcgccac ggtgccggtc ccggccgccg aggagagcga tgcccgttga gcacggctgg
     2881 gcgcagctcg ccctcgagcg gggactcgtc ccggacgtcc tcgtccgtcg caggatccgc
     2941 gagctcctgc gccggcggct cgccgaggag cgggccggcg acgccgaggc ggcgctcgag
     3001 cgcgaggagc ggctcgtcgc ccagatgcgg gagagcccgg tggcgctcgc gacggccgag
     3061 gcgaacgagc agcactacga ggtccctccc gagttcttcg agctcgtcct cggcgcgtac
     3121 cgtaagtact cctccggcct ctggtcggac ggggtgacga ccctcgacgc cgccgaggcg
     3181 gcgatgctcg ccctcacctg cgcgcgcgcc cgcctcgagg acgggcagaa cgtcctcgag
     3241 ctcggctgcg ggtggggctc gctgacgctc tggatggccg agcgctaccc cgcgtcccgg
     3301 atcgtcgccg tctcgaactc gaggagccag cgcgagtacg tcgaggcccg ggcggcggag
     3361 cggaacctct cgaacgtgaa ggtcgtcacc tgcgacatga acgtcttcga cccggcgacc
     3421 ggcccggcct tcgaccgcgt cgtctccgtg gagatgttcg agcacatgcg caactggccc
     3481 gagctcctcg gccggatcgc ctcgtggctg aagcccgacg gacggctctt cgtccacgtc
     3541 ttctcgcacc gcgacgtctc gtacccgttc gtcgcgaggg acgagagcga ctggatggcc
     3601 cggagcttct tcaccggagg cctcatgcct tcggaccacc tcctcctccg cttccccgag
     3661 cacctcttcg tcgaggagcg ctggcgggtg aacggaacgc actacgcgcg gacggcggag
     3721 gcgtggctcg agaacctgga ccggagacgc gccggagcgc ggaagctctt cgagaaggcg
     3781 tacgggcccg ccctcgcgcg ccgcaagctc gccgagtggc gcgtcttcct catggcctgc
     3841 gcggagctct gggcgttcgg gggcgggaac gagtggatgg tctcccacta tcgcctgcgc
     3901 cgccggtacg tctgaggcgc gcggcgggtc gccccggcta cactccggac gaacgacccg
     3961 gaggtgaacc gtgctgttcg acaagagcaa gctccgcctt cccgattcgg aggacgcgct
     4021 ggaggggcgc ccgacgccgc tcgccgtgac gaaccgccac ttcgtgaacg gtcgcgagat
     4081 cctcccgccg ttcccggccg gcctcgagag ggccgtcttc gggatgggct gcttctgggg
     4141 cgccgagaag gcgttctggg ctcttcccgg cgtccacacc acggccgtcg gctactccgg
     4201 cggcctgacc ccgaacccga cctacaagga ggtctgcacc ggccgcacgg ggcacgccga
     4261 ggtcgtcctc gtcgtcttcg acccggcggc ggtctcgtac gaggcgctcc tgaagctctt
     4321 cttcgagaag cacgacccga cgcaggggat gcggcagggg aacgacgtcg ggacgcagta
     4381 ccgctccctg gtcctcacgc actccgcgga gcaggaccgg ctcgccaccg cgatccggga
     4441 cgtctaccag gcggtcctct cggcgcgggg cttcgacccc atcacgaccg ggatccggcc
     4501 ggcggggccg ttctactacg ccgaggacgc ccaccagcaa tacctcgcga agaacccggg
     4561 cggctactgc ggtctcggcg ggatcgggat cgcctgcccg gtgggcctcg gcgtcgacgg
     4621 gtaggcgccc ggggggcgcg gcgtcagccg cgcccctggt gggtgtcccc gagcagtccg
     4681 cgggcgagcg ccggaagcag gacgagggct ccgaccatgt tcatcaggaa gaggaagcag
     4741 aggagcagcc ccatgtcggc ctggaacttc agcggcgaga agatccacgt cgcgacgccg
     4801 agcccgagcg tgatccccgt gaagaggacg ccgtttccgg tgatcgcgag cgtcttccgg
     4861 taagcctcgg tcagcggcac gccgtcgcgg tagagcgagc ggaaccgggc gaagatgtag
     4921 atgccgtagt ccacgccgac gccgacgccg agcgcgacga cggggagcgt cgacaccttc
     4981 aggccgatct tcagccacgc catgagcgcg taggcgagga gcgagacgag ggcgagcggg
     5041 acgaggatgc agagggtccc ccggagcgac cggaacgaga ggaggcagag gacgacgatg
     5101 gccccgaaga cccaggcgag gatcgggaac tgcgccgcgg agaccgcctc gttcgtcgcc
     5161 gccatcaccc cgacgttgcc cgtcgcgagg cggagcttca cgtcggggtg gccgtggtcg
     5221 gcctcgaagc gcttcacctc cgcgacgatc cgctcgatcg tgtcggcctt gtggtcggcc
     5281 gtgaacatca tcaccggcat gacgctgcag tcggagttga ggaggccgga ggtcgtcggg
     5341 acgtaggtga cggactgctg gaggacggac tggttgcgcg agaggaccct ccacttcggg
     5401 gacccttcgt tccaccccgc gttcagcatc ttcgcgatcg cggggagggc gatcgtggac
     5461 tggaccccct cgacgttctc catgtgccac gcgaagtcgt cgatggtggt catcacggag
     5521 tggtcgacgc acccctcggg cttcgtctcg acgatggcgg tgaggatgtc gaccccgatc
     5581 gagaacttcg acgtgatgac ggcgctgtcg acgttgtagc gcgagtcggc tcgcagctcg
     5641 gggacgcccc ggtggaggtc gccgaccttc acgtcgcgtc ccttccacca gccgaggccg
     5701 aagaggaggg ccgccacggc gacgatcacg gcggcgggct tcggcgcgga gacctgcgcg
     5761 agccggcgcc agaggccgga catgtgctcg gcccggcggt ggaggcgggc gttgtagtcg
     5821 gcggggtagt gcaggtagga gaggacgacc gggatcagga agaggttcgt caggatgatg
     5881 acggccacgc cgaggctcgc cgtgatcgcg atctcctgga tgacccggat cttgatgagg
     5941 aggatcgtca ggaacccgat cgtgtcggac gcgagcgcga tgaagcccgg cacgaggagg
     6001 cgccggaagc tgttgcgcgc cgcggtcatc gggtccgcgc cgtcgaagat ctcggcgccg
     6061 ttcgcgctga tcatctggac ggcgtggctg acgccgatcg cgaagatgag gaacgggacg
     6121 aggatcgaca tcgggtcgat cccgaagccg aggagcgtca gcaggccgag ctgccagacg
     6181 acggcgatcg tcgagcagcc gagcgccacg agcgagagcc ggtgcgactg ggagtagagg
     6241 tagacgagga ggcccgtgag ggcgtacgcg acgaggaaga agaggatgac gcggcgggtg
     6301 ccggcggaga tcgcgccgac gaccttcgca aagccgatga tccgcacgtc caccttggag
     6361 ctcgggacga actccgcgtc gaactgtcgg cggaccttct cctcgagggc gttcgagacg
     6421 gcgatgaagt cgagcttctg cccggtcgaa gggtcgacct cgaggagctc ggcgctgatg
     6481 atcgtccccg agaagtcgtt cgccacgagc cgtccgacga tgccggcctt caggacgttg
     6541 cggcggacct gcgcgagacc ctcggcgtcc ggctcgaagt cgtcggggat gacgttcccg
     6601 gcggcgatgc cgtcctcgac gacctcggtg tagcggacgt tcggcgtgaa gagcgactgc
     6661 acccggctcc ggtcgacgcc ggggatgaag aagacctcgt cggtcgcctt cttcagcgcg
     6721 tcgaggaact cgggcgtgaa gatgtcgcct tcccgcgcca tcatcgccac gaggacccgg
     6781 ttcgcccccc cgaactcctg ctggtgcttc aggaacgtct ccatgtaggg atgggcgtgc
     6841 gggaggttct tctggaagcc cgcgtcgacc ttcaggtgag ccgccgacca ggccatgaag
     6901 agcgtggccg cggcgaagag gccgaggacg acgggccggt ggccgaaaac cgctttctcc
     6961 aggaagctcg tgatccgttc cttcgtcatc tcttcccctc acccctccgc cggcgccccg
     7021 ggcccgagcc cgggagcgcc cgccctagaa cggcttctcg atcggccgga ccccgccctc
     7081 tccgaggagg acgatcccgc ccgcgcctcc cggaaggatc gccagcgagg cctttcggtc
     7141 ttcctgctcc cgggcccgga acgtgcggcc ggcgtcttcg ctgaagagga ggacgccggc
     7201 caggcctgcc acgacgaccc ggccgccgcc gagctcccgg ccggtcatca gcgtcgcttc
     7261 ggtgccggaa gcgacgccgg tccaggtcgt ccctcgatcc tcgctgcgga agaggtgccc
     7321 ccggagcccg aaggccagga ggcccccgtc ggagagcgaa agcaggccga agaacgagcc
     7381 ctcgtagggg gacgggagcg ccttccagct ctcgcccccg tcgtcggagc ggtagaggga
     7441 gcccgcctct ccggcgagaa tgagcgcgcc gtccgcctcc gcgatcgcgt tcaggtgcgt
     7501 gtcgtccccc gcgacgaccg tccggctctc ccacgtgtcg ccgccgtccc cggtcgcgag
     7561 gaggaggccg taagcgccga tcgcgaagcc ctgtctttcg tcacggaacc agacgtcgag
     7621 gagcggcctc tcctcctcgg gagcgtggcg gacgcgctgc caggtcgccc cgccgtcgcg
     7681 cgtccggagg atggtctcgt cgtgtccgac cgcccacccg aggcgggagt cgtgcaggaa
     7741 gacgccggtg aggagcgctc gcgtcggcgc ctcggcctgg gtccacgacg ccccgccgtc
     7801 gcgggagacg aggacgtgcc ctcgctcgcc gaccgcgacg gcgaggccgt cgcggtcggc
     7861 ggcgtccagg aggagcgagc gggcggcgag ccgggcgggc gtcgaccgtt cggacgcggc
     7921 gggaggctcc gccgcgcccg caggggccag ggtcgccagg gcgagcaggg ccgcgccgag
     7981 cgcgggaacg gacttctcac gcatggcgcg gccctccggg tcagcggacg ccttcgcgcc
     8041 ggagagcgtc cggcatgaag tcgccctcgc tccgcttgat cttgaagtcg tgcggcgcgt
     8101 tctcgctgtt caggcccatg acgaggtagc gccccgcctg gaggtcggtg tgggactcgg
     8161 cggaggtcca gatcgtcggc agatcgtagt aggcgatggc gtgcccttcc gagacgcgcc
     8221 agagctggtc acggttgtcg tactggtcga cgacgaggat ctgccagctg tcctcgtcga
     8281 tgtagaaggt ccgacgcttg tagatgtgcc tctcgccctt cttcagagtc gcgtcgacga
     8341 cccagacccg gtgcagctcg taacgggtgt ggtcggggtt catgtggagc ggcgtgagga
     8401 tgtccttgta cttgaccttc gggtcctgga gccggtaggc gttgtagggg acgtagatct
     8461 ccttcttccc gacgagcgtc cagtcgtact tgtccggcgc gccgttgaac atgtcgagct
     8521 ggtcgctcgt cctcatgttg tcggcggccg tgccagggtt gtcgtaggcg acctgcgggg
     8581 cgcgcctcac gcgccgctgg cccgggttgt agagccaggc gtcgcggggc tgcgcgacct
     8641 ggttcatcga ctcgtggacg aggaggatgc ctcccgcgag gcgcgccggc gcggtgaccg
     8701 cctgcttgaa gttgaggagg cggttcttca tcgaggcctc ggtcgtgccg gggaggtggt
     8761 agacgaacat cgtctcctcc tcgaactgca cgagcgtgta ctggcctcca cgcgtcgggg
     8821 ccgcctgcgc gacggtgcgc cccgcgatct ccgcccggta acggaggagg tggttccaga
     8881 tgacctcgat gccgctcttc gggatcggga acgggatgcc gccgacggcg ccggtcactc
     8941 cgttcccccc ttccgcgagc ttcgcggtcg tggcgacctt cttcgtggcg tcgtagatcc
     9001 gctgcggcgc cgcgccggtc cggtgcgtcg ggtagaccgg catccggaag gaggggtacg
     9061 ccttcagcat cgccttgtgc ccttcggaga gcttcgcggc gtgctgcgcg gcgttcttgc
     9121 cgtcgatcgt gaagaggggc ttctccccgg cgaaggggtc ggcgtagtgg gtgcccggct
     9181 tccagcccgc cggcgccttc gtcaggccgc cgtcccaggc cgggatcgag ccgtcggcgt
     9241 tcccggcctt ctcgccgccg agcggtgtga gggacgcgcc gagcttcgcg gcgtcctgcg
     9301 gggagagctg ggcgagggcg ctcccgccga ggagggcgga gagtgtcagc gtgcagagga
     9361 ggattcgggt gttccttcgc atttcggctc caccttctct agaaggagta cttcacgttg
     9421 cccgcgagga agtcgcggtc gttgatcagg ttgtaccgcc ccgcgccgaa gtagcgggtg
     9481 tagctcaggt cccactcgac gttgatccgg tactggaagc cgaggccgag cgtcagcgcc
     9541 atccggtcct cgatgaagga gccgcccggt ccgggcgaga ccccgttcac gtcgtgggcg
     9601 aaggagaagc gcggggcgag gttgatcgcg ccgatggcgt tgctgtagtc gagccggccg
     9661 gccacgacgt agccccagga cgagctcgtc gggaacgcgg tggagggctc ggtgcccggc
     9721 tggacgcccg cctgctggtg ggccgggttg ccgctcgtgt aggtgcccgg cgcctcgagc
     9781 cggaggacgg actgctcggg gaggtcgtgg accttgctcc aggcgccctc ggcgacgagg
     9841 acgagctggt cggcgccgag gacgcggccg aagacccgcg tcgcggtgag ctgggcctga
     9901 gtcgtgtcga agagccgata gccgcgaatc tcctcgtcga agccgtaggc gccggcctgg
     9961 ttcgtcgcgg ccaggagccc tccgagctgc cggagcgggg cgagctgcgg cgccgccgga
    10021 aggagccgga ggggcgtgag cgccgcgtag aggatctcga cgtcgtcgac ctggaggggg
    10081 acgtccttgc gatgggagac ctctccctgg agcgcgacgc cggtgcttcc gagctgggcg
    10141 ttgaagctga ggccgtagag cttgatgtcc tccgggtagg agaggaagta gctcgcgctc
    10201 gccgcgtagt tcccccgggt gaggaggccc gcctgggtcc cggtgcgcgc catgatgaga
    10261 ggcagccggc tgtggtagtt catgtagaag aggccgaact cggtcccgcc gagggcgggg
    10321 acgaagagcc ggaacgcggc gccgtactgg ccggagtcgt cggcctcccg gtcgccggtg
    10381 cgcgggacgg cgacgccgac cgggttgttc gggatgttga agccgacggg gatggagtcc
    10441 ggggcggagc cgaacccgag catgaccttc gtggccccgg cgcccgcgag gtcggtcgtg
    10501 gagaagtagc tcccgaccgg gtcgatcttc gtctgggtcc aggcgtactg ccagaacccc
    10561 tcgagggacg tgttcttcga gggcttcacg ctcaggagga cggcgcccac ggggaggagg
    10621 gcgtcgcgca gctcggagcc ggggacgcgc agggcgctca cgtcgaccgg gttgatcgcg
    10681 ttgatgccgc cctggatgaa cgtgctctcg ccccagttga tgacctgcca gccggctcgg
    10741 atctctcccg ggacgggccc gaggtcgaac ttcgcccaga ggaaggcatc ccggatctcc
    10801 gcccggctgc cgacccggtc gagggcctcg gggctcagcg gggtgcggtc gcggtcgcgc
    10861 tcctcgttct cgatgtcgta gaagccgtag gccctcacga acgcgccgat ccgcttgtac
    10921 gtcagctcga gctcggtcgt cgccttgaag gcgctgctcg cgaggcccgt gtcgtagttg
    10981 aggttcccgt cgtcgccgtt cacggagaac gccgtccctc cggacgcgag gccgacgagc
    11041 gcacggtccc tcttctcgac tcgagcgaag agaccgtacg acaccgtcgt gtcccagctc
    11101 caggagaagt ccccctgctt cccgtcgatc gcgggtgccg tccgggccag gggggccagg
    11161 aaggccgcgg cgagtgccgc aatcgcggca ctccgtgtcg tcattcgaaa tgcgctcatc
    11221 ggatccccct tcgccaatct gcgggcttgg cgcggagggt aacccagggg actcggattt
    11281 cgcacagaaa ccgtgctcgg cgtcacaaac gcgctaacgc gcgaccaaag agatgtgctg
    11341 cgtttacgca gcgaggggac gcggtccggg ccgccgggtg cggcccggac cggacgcccg
    11401 cctcaggggc gcgcgtagct gtcgatgacc ttctcgatcg ccttgcgaca aacggggcag
    11461 aagccgcctg tcgtccgggt gaacatgatg cagtcggccg aggagcggta gagccccttc
    11521 gcctcgtagg cggccccctc gaaggcgccg accttcccgg caaacctctc cttcgcgaag
    11581 aaggccgcct ccgccttctg ctgctcgcgg aagagggcgt cgatctcgga gggcgggacg
    11641 cccttcgcga ggagggcctt ccgccgggcc tggtactcgc gggaggcctt ctcgaacgcg
    11701 tccttcgacc agggcgtcgg aagcggcgtg ccggcttcga cgagctcctt ccacttcggg
    11761 ttcgccgggt ccttcagcgc cgtcacgttc atctcccacg gctcggggag gtcggcggcg
    11821 cccgtctcgt aggcgacgtc cgaggtgtag tactcgtcgg cgagagcggc gaagtggtgg
    11881 ccgaactcgt ggacgaagac gtagtccgcg aacgcggtgt cggccgaggc ggtcgcctgg
    11941 ttgttgtaga tgccgccgcc tccgtactgc gcgtcgttca cgaggatctc gaggacgtcg
    12001 tacggggcct gggccgccgc gtcgcgcagg gcgcggtcgt cgtacgtcag gacgtagcgc
    12061 tcggagccga agacgttgta ctggacctgg agcggcgtcc ggcggaagag cttcgactgc
    12121 ggccggtgcg cccctccgtc gccggaagcg aggtcgaggg cccggacgtt gaagtccccc
    12181 ttccgcgacc ggaacggctc gtatcggaag agcgtgtcga ccattcgctt cacgtccgcg
    12241 cggaacttcg ggaggtcctt ttccgcgtag ccctcgccga ggacgaggag gtcgaccttc
    12301 tcggtcgcgg ggccgctctc gaagacggtc cagaccgtgc cgaccttcgg gggcggggcc
    12361 gggttcacgt cgggcgaagc ggggtcgacg agcgttcccc agacctcccg gaagaggttc
    12421 tgccggtcgc gcttcttcag ggcgacctgg acgggacgga gcggccaggg gaagcgcagc
    12481 gactcgcgga acgtccggtg cgtcgccttc gcctcggagg tcgtctccca ttcggcgtag
    12541 atcgaggaat agccgcgggc gtagaggagg cgaccggtcg acgggtcgag gacctcgaat
    12601 ctgtacgagc cgaggccgag gccgtcgtcc gccttcgtga ggcttccggc ccaggggccg
    12661 tcgttcacga cgcggtcgac gacgacgatc tcggccccct ttcccccggt gtggaggagg
    12721 tcgacgcgca tcgtctgagg ggtgaagtcg tcgaggaggg cgggcgcggc gccgcttccc
    12781 ggggcggcga ggggggcgag caggaggagg gccgccccga aggcggcgcc gtggaagtgc
    12841 tggggggtgc gtctcatgcg gacctcgcga ggatcgtagc ggagcggggc tattctgacg
    12901 ccgtgattcg gacgttcgtc gcggtgccgg tcgaggacgc ggtcgtgagg cgccgcctcg
    12961 ccggcgcacg gagcctcctt cccgagctcc ggggcgtgcg ctggacggcg gagaggcagc
    13021 tccacttcac cctcaagttc ctgggcgacc tcgaggcgga gcggctcgcg gacgcccgcc
    13081 aggccacggc ggcggcggcg cacagggggc cgggctcgtt ccggctcgcg ctcgaggggc
    13141 tcggagcctt cccgccgcgg ggcgcggcgc gagtcgtctg ggctggctgc gggtcaggcg
    13201 caacggagct cgcctcgctc gcgggcaccg tcgaggaagc gttcgaggcc gcggggtttc
    13261 ctcgcgagga acggccgttc tcgccccacc tgacgctcgc ccgagtgagg gaccctctct
    13321 ccgggcggcg cctcgcgcag gcgctccctt cggtgccgg
//
LOCUS       DAOONL010000090        13097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5242_length_13097_cov_9.153581, whole genome shotgun sequence.
ACCESSION   DAOONL010000090 DAOONL010000000
VERSION     DAOONL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5242_length_13097_cov_9.153581"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1511
                     /locus_tag="PLP50_12500"
     CDS             237..1511
                     /locus_tag="PLP50_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPA52409.1"
                     /translation="MSRRAAQKLTKRFIDDLEPGKKGGTWYGDADLPGLYVVSYPTGA
                     KVFIVRFLAARRRRAVVVGKYGILTLEKARQKAKMLLSDATLGGDPATARDRARRTPT
                     FATWADTYLSRVHLEKKSPRDDEKFIGEKSSKGKALRARWGSKPLDTITPEDVAAFRD
                     GLRREKRKTGDGGGETAPAGPKGPPPTTANRWLASVRSCFNAAVREGHLAANPARSIP
                     PFRENPPRARVLSTDEMERLLRALARESDPHIRAAFRLLIETGARLSEVLGARWADID
                     FESRLWRIPSPKAGRPQVFPLGERTVEMLRKLPRVGAFLIPPLRQFAKPGSEKKRERT
                     ERPRADLRGPWDRLRQRAQLEDVRIHDIRRSFGLEIARKAGLHVASKLLRHSSVKVTE
                     QVYAPLGIDELRDAVEKRADVLPFPDQMRNAG"
     gene            3098..3262
                     /locus_tag="PLP50_12505"
     CDS             3098..3262
                     /locus_tag="PLP50_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52410.1"
                     /translation="MRLWRYQGTGPRYVKPSRVRCLYAEADLEAFMRERTYTSTAEET
                     VSRKVAAAGA"
     gene            3479..3694
                     /locus_tag="PLP50_12510"
     CDS             3479..3694
                     /locus_tag="PLP50_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52411.1"
                     /translation="MTPETTALLAFDVNEVLRLAREADARRTPADRSRAADALERARF
                     EAAEFLVDRGNPAGARFAAGWLLRRVA"
     gene            3691..5313
                     /locus_tag="PLP50_12515"
     CDS             3691..5313
                     /locus_tag="PLP50_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HPA52412.1"
                     /translation="MNRAPRLVDAEELARLQRKAAARVLADGGELPWTEPRPLPPIAP
                     PVPTLPGDLLPVPLRPWLADVADRVSIPLEFVAAPAVVALGSVVGRTVAIRPMARDDW
                     QNPANLWGGIVGPPGTMKTPAISEALRPLRRLVAEARNRFEDAGATREADLEALGAKR
                     KAMRRRLELAAKRGDEADLARFRDELKDLKAEEDAGEVRERRFTTSDATIEKLGPLLN
                     ENPRGLLVVRDELTGWLRGLDREDRAQDRAFYLESWNGTGGFTVDRIGRGTLHVEALC
                     LSVVGGIQPGRLSAYVRGAIGGEEDADGLLQRFQVLVWPDEFGDWRGVDRWPEKEARE
                     RAFRIFDRLANAGTLDLGANAPEEDLPFLRFAPDAQGLFIEWRHDLERRIRSEEVKAA
                     PAFEAHLAKYRSLFPKLALIFHLVDVADGAGPGAVTLKAAQLAAAWTEFLESHARKVY
                     AEELAGGVSAAHALAEKITRGKVTDGMAVRDVARREWSGLRTSGRVFEAAETLEGLGW
                     LRVESVDTGGRPSDVIRLHEKFRGIDPEEEAS"
     gene            5484..5729
                     /locus_tag="PLP50_12520"
     CDS             5484..5729
                     /locus_tag="PLP50_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52413.1"
                     /translation="MEEDPGDVLCGACYAARRGPGEVLAFDPDRRSRTLARLADRPCP
                     DCQAVTWHVNGRGDATCQTCARRRAGFDEMSAREARP"
     gene            5839..6549
                     /locus_tag="PLP50_12525"
     CDS             5839..6549
                     /locus_tag="PLP50_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52414.1"
                     /translation="MRRRPKRTLQTLPAARADSRAEEEGLATRQEDPEEDARIQATAD
                     KLLETNTREELLHVISTLRSARGPRFMKHPKREGDVAFHGTPEEHQASLIEATGCSDL
                     ETADWLLREVASTFLNSMSARERLNAAGRMLAALAPRSPLEGLLCSQIVAVHGLAMRL
                     INIGAASEIGFVHLSPSLDGANKLLRTFTMQVEALQRLRTAGVTEQRVVVQHVTVADG
                     GQAVIGAVSRAGGEEGGR"
     gene            7094..9727
                     /locus_tag="PLP50_12530"
     CDS             7094..9727
                     /locus_tag="PLP50_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA52415.1"
                     /translation="MKQIAVVMMLASITGLTTTSRQVVADEPVSRTPLAPAGHSEPEH
                     RSDARLALFRSEPASQVLVVELPDARTALPDEIVALQDEARAGRTPTRNGFRRELPQG
                     LDVDLAAPPAAQSSGVVRPGLLATLENGSVAWAGSFRARGAWALRIQLTNVSVAPGAR
                     FLVCGTTQSDCTAFGIEAVTDRGEIWSPIVSGDLLHLEVTVPAPGSGASFGGFVPSAL
                     MEVLAPDVLLPEAREEPRIIGALDTSCMMKGECVSDSTLADLSKYRSAYAHLQFVEDG
                     ANYICSGALLNDDGVDGFIPYLLTANHCFAKQASASSLVAYFDYRYASCSASAPTTLS
                     GFPKVYGASLLATGATSDFTFLRLSGQASGYRYYLGWDPRTIPSSSMIYRLSHPSGLP
                     QHFTASQFTPSPSTYCSALPTSRFAYSMATTGGTAGGSSGAPALSTGGYIVGQLYGVC
                     GSGDLKDPCDPRNFDVDGSFSSTWPSVSQWLRPTSGASRPVPDFGYSPSSPRVGESIQ
                     FTDFSKNGPTAWTWSFGDGSQSTAKNPSHAYSAAGTYTVTLTAGNTAGAASTSSTVTI
                     SSTPLQPTISYFGANPPAIAAGQTTTITWTSTGGTSAFINQGVGQVPTSGNRTIVPVT
                     GAPYTLTVSGPGGTAQATLTISSVGTTYGNAWIVPSTARVHGDGGAFWTTDVTVMNPE
                     STAVGVNLKFLGHGESGLSGPESTFTLAPRSSRVIQDVLLAEFGLSSDWGPILIRTVT
                     GNVVVQAQTSTPGNGGTYGQSVPAISRSSAIGTSPRAICGIRQDAGYRTNIVLVNLAE
                     QAAQIDLVLLSPTGSVLASIQETLGPLGFRQLNVANDLRISNLAGGTLVLSSKSSLTP
                     FAAYASVIDNRTADPRTLLPQ"
     gene            9805..10608
                     /locus_tag="PLP50_12535"
     CDS             9805..10608
                     /locus_tag="PLP50_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52416.1"
                     /translation="MTLLLALPVPSRAQSLIDLEYTPRAADEDIRQAVELLDDDGPIA
                     FSLRFAREGAPAPGSEEIGRIRVGTPAVRLYARQSVVSFLQGAATTQFKDWGHPLADN
                     AGVRVEANVRRFFVDESYVYKAEIAIGYVVRGPDGSVLWNAEIDSDAKVFGVARKAPN
                     YREALSDAFRKNLALLLARPGFRAACTGKRGSKADTAGPTVTPAEARLAVLRLIKEGL
                     GDDLLVAYVRQARISPAFSADDILEWKSAGIPASVITAALGAAAPAPEP"
     gene            10945..11955
                     /locus_tag="PLP50_12540"
     CDS             10945..11955
                     /locus_tag="PLP50_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52417.1"
                     /translation="MTREAIATIISGLPPFVQQLGWHQALLDGQRIQEGLAYLNDRSH
                     FVFAALLLPGAAVAALILARVHDTVAALRDDGILRDDDATIRETYQRFHRLANHWFFR
                     LAALCLGGVTALVFLAFRNGDAHTSWWGHRQFGPGGLAFAFIEAAMVYFGTQTMVLTA
                     FASAMFARLLAGRIAFRPFHPDGANGLAPMGRMIMMLWILALVLGAEICVALFLGYLG
                     IEKMPLTWLLALAGLAAIPALAIAPLYASLHAIHRARIARISHLEPLLNSMLERAEGQ
                     VDEGRFDDASETLATLGDMQGVHESFASLNVWPFNPRALAGVVAVYALQLVLTLKEVL
                     GF"
     gene            complement(11933..12679)
                     /locus_tag="PLP50_12545"
     CDS             complement(11933..12679)
                     /locus_tag="PLP50_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52418.1"
                     /translation="MRGSRTAPDVIAFLAVVAVQSVAWAVVGYLSQAPRREIVGALVA
                     GASVGVPLGLGFDLVIGAERHVFGYRLPLTPAFLVANGALSYGLAIATVIHLWRPAAS
                     GPPKRSRPAGLLAVSLAGGLVAMVLVADPGLLAAVFLIGGAVVLAGEGLSLLLGREGP
                     FAAVAAGRFRPAARLWLSSVALGVVYEAANALWPVWAWGAAALMPKPWWSVILVGCGY
                     VVLFHPMWLLAEAVGPWILRRRSLRSPEPP"
     gene            complement(12792..>13097)
                     /locus_tag="PLP50_12550"
     CDS             complement(12792..>13097)
                     /locus_tag="PLP50_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52419.1"
                     /translation="RPDSIRMIPGREFGHSAPPRIGGHTRTGKNEVRRTLTHRLQKGW
                     REGRVDAKTARQYIAVAEGCGLKRQAGIAELTGELFGRTLLELEGGEIIGACPGCSE"
BASE COUNT         2157 a         4663 c         4258 g         2019 t
ORIGIN      
        1 gtgccggagg agggactcga accctcatga gcttgcgctc gggggatttt gagtcaccgg
       61 gtggaccgtt ttccggggta tcttgcccgt agaggcgcgt tcagatctcc gctttctatc
      121 agtctccttg cgttgatcgt ccaccgggac catactctcc gccgttaggc aatccgaagg
      181 cacgccgcca cctcgaaggc acgcagaagg caaggcgagg ggaacaggga gcggacatgt
      241 cgagacgcgc cgcacagaag ctcacgaaga ggttcatcga cgatctcgaa cccggaaaga
      301 agggcggcac ctggtacggt gacgccgacc tccccggcct ctacgtcgtc tcgtacccga
      361 ccggcgcgaa ggtcttcatc gtccgattcc tggccgcacg ccgtcgccgg gcggtcgtcg
      421 tcggcaagta cgggatcctg acgctcgaga aggcccgcca gaaggcgaag atgctcctct
      481 ccgacgcgac gctcgggggc gaccccgcga ccgccagaga ccgcgcacgg cgtacgccca
      541 cattcgccac ctgggcggac acctaccttt cccgagtcca cctcgagaag aagtcccccc
      601 gcgacgacga gaagttcatc ggggagaaga gctcgaaggg gaaggccctc cgcgctcgct
      661 gggggagcaa gcctctcgac acgatcaccc ccgaggacgt tgccgcgttc cgagacggcc
      721 tcaggcgcga gaagcggaag acaggggacg gcggtggcga gaccgcaccg gccggcccaa
      781 agggccctcc cccgacgacg gcgaaccgct ggctcgcctc cgtgcgctcg tgcttcaacg
      841 cggccgttcg agaggggcac ctcgccgcga accccgcgag gagcatcccc cccttccgag
      901 agaaccctcc ccgggcacga gtcctgtcga ctgacgagat ggaacgcctt ctccgggccc
      961 tcgcccgcga gtccgatccg cacatccggg ccgccttccg actcctcatc gagaccggag
     1021 cccgcctctc cgaggtcctc ggcgcgcgct gggcggacat cgacttcgag agccggctct
     1081 ggcgcattcc gtcgccgaag gccggccggc cgcaggtctt ccccctcggg gagcgaacgg
     1141 tggagatgct ccggaagctc ccgcgggtcg gcgccttcct catcccgccc ctgcggcagt
     1201 tcgcgaagcc cggaagcgaa aagaaaaggg aacgcacgga acggccgcgg gcggacctga
     1261 ggggcccatg ggaccgcctt cgccagagag cacagctcga ggacgttcgg attcacgaca
     1321 tccgtcggag cttcggtctc gagatcgcac ggaaggccgg cctccacgtc gcctcgaagc
     1381 tccttcggca ctcgtccgtc aaggtgacgg agcaggtgta cgccccgctc ggaatcgacg
     1441 agctgcgcga cgccgtcgag aagcgcgccg acgttctccc cttccccgac cagatgcgga
     1501 acgccgggtg aggcccggct gagaggagcg cccacatggg ccagaagacg agtgaggacg
     1561 ctcctcgatc tcggactccg agtcctcggc cgggcagaac agaacgccca tttcgacggg
     1621 cggcgcctct gggagctccc ggactccggg gccggggcgt agcgacgcgc gacgcgggga
     1681 gagaccgcag ggcgagctat tgaccatccg ccggcgggga cggcacgcct gatcagcgcg
     1741 ccgaagcgat ctcgctcccc cgcactcccc gccagcgggt tcttcctcga ggggggcagg
     1801 cgggagcccg cagtggacga ctcgagggac gccggaaagc accatgagcc gacgaaggcc
     1861 aagacgcagg agcggaggga gtcccgcact tccgagcagg ttgaggatgg cgacgccttc
     1921 agcatcgagc gggctttcag cgaagaggtt gcgacctgtg cagcccagtg gagcgacgcg
     1981 gggcctaaga agcggctgcg gcttctgctt gatgcgttct tcgggtgtgg cgcacgcctc
     2041 agctccgagg cggtcgcgga gttgatcgtg tctgctcgcc ttctcgcgct tgaggccgga
     2101 ggcgtcgacc acagcactcc acgcctcctg agaagggccg cgctcgccga agagcgactc
     2161 gcggaccgtc tggccgccgt gcgggattcc ttcggctccg acatcacctg ggcggagctc
     2221 gaatccgagc tcccagccat agtcagcgaa gacccggagg ttctctgggc tcccacaatc
     2281 cttgccgcgc tcgaggcgtt gcttctcctc ggcgcccgca cgcttcctgc ctatgacatg
     2341 tcggaccgcg cccacgagga tctgcggatc gtgtcgggcc gcgcccgcga ggacctgcgg
     2401 cggctggcga cggaactcga tctctcgcgg ccgtcgcgac gtcggaaggc gaaggttcag
     2461 gccgaaagac ttgcggacgc tcggggggtg ctcgcgatga tcaccgaccc cgacggctac
     2521 ggtgggacgg cccgcctggc tcttcgcctt gtgaagaagt gtcgcacgga gagcgccgca
     2581 agacgtgttc tcgagaccac gcccgagtgg gctgggctct attccctccg catgacaagg
     2641 gacctcgcgg tgaagaaggg catacgcccg ggaagtcgga acctcgccct gattctccac
     2701 gcactcgcgg ccttccgctg ggtgcctgga cgacgagggc gagtgtcgga acgcaacgtc
     2761 gccgccgccc tactcctcgt gtggcgcgac gcggtcagcc gtggggttga tgctggccac
     2821 cgatccctga gccgcaaaga gctcgcgcgt agggcgaccg cgcttcgcag aggcgccgag
     2881 agcgtcgtcc gcgagtcgac gaggcccgcc cgggccgcct cattccggcg ggaggaaagt
     2941 tcgctgcccc acggaatgag gccttcctcg ttcgctgtcg atgctggaaa ttcgctggcg
     3001 gccagagaag gctcaacgcc gaaaggggtc atcaagaatg acgacctact tgaccacgaa
     3061 cgaggccgcc gcccggctcc gcgtcgcacc ccagaccatg cgcctgtggc gctaccaggg
     3121 gacagggccc cggtacgtga agccgagccg cgtgcgctgc ctgtacgccg aagcagacct
     3181 cgaggcgttc atgcgggaga ggacgtacac ctcgaccgcc gaggagaccg tcagccgcaa
     3241 ggtcgccgcg gcgggcgcgt gaggaagccg ccatgacgag aatgaagagg gccggctccg
     3301 ttgcagcgga agaccggccc gagcgcagga aagacagcag cgacgccgta attctacgcc
     3361 atcgcgatcg tacctccccg cgcaaccggc ggcgtctcgc cctggtctcg gcgatggacg
     3421 agtctccggc ccccgggcgc tggaaacgtc tcggcgacgt cgtgatgggg gcaacgtcgt
     3481 gacgccggag acgaccgccc tcctagcctt cgacgtcaac gaggttctcc ggctcgcccg
     3541 ggaggccgac gcgagacgga ccccagcgga ccgatcgagg gccgcagacg cgctcgagcg
     3601 cgcccgcttc gaggccgcgg agtttctcgt cgaccgcggg aatccggcgg gggcccgttt
     3661 cgctgctggc tggctgctcc ggagggtcgc ttgaatcgcg ccccgcgcct tgtcgacgcg
     3721 gaggagctcg cccgccttca acgcaaagcc gccgcgcgcg tcctcgccga cggaggagag
     3781 ctcccttgga ccgagccgcg gccccttccc ccaatcgctc cgccggtccc gacgcttccg
     3841 ggcgacctcc tcccggtccc tctgcggccg tggcttgccg atgtcgcgga ccgcgtttcc
     3901 atcccgctcg aattcgtcgc cgcaccggcg gtcgtcgccc tcgggtcggt cgtcggccga
     3961 accgtcgcca tccgcccgat ggcgcgcgac gactggcaga accccgcgaa cctctggggc
     4021 ggaatcgtcg ggcctccggg aacgatgaag acgccggcta tctccgaggc cctccggcct
     4081 ctccggcgcc ttgtcgccga ggcgcgcaac cgcttcgagg atgccggggc cacacgggag
     4141 gccgacctgg aggccctcgg ggcaaagcgg aaggcgatgc gccgcaggct cgagctcgcc
     4201 gcgaagagag gcgacgaggc cgacctcgca cgctttcgcg acgagctgaa ggacctgaag
     4261 gccgaggagg acgccggaga ggtccgtgag cgccgtttca cgacctccga cgcgacgatc
     4321 gagaagctgg ggccgctcct caacgaaaac ccgcggggcc ttctcgtcgt tcgggacgag
     4381 ctgaccgggt ggctccgtgg cctggatcgc gaggaccgag cgcaggaccg cgccttctac
     4441 ctcgaatcgt ggaacggcac gggcgggttc accgtggacc gaatcggccg agggacgctc
     4501 cacgtcgaag cgctctgtct ctccgtcgtc ggtggcattc aaccgggccg gctctcggcc
     4561 tacgtgaggg gcgctatcgg gggcgaggag gacgctgacg gcctgttgca gcgctttcag
     4621 gtcctcgtat ggcccgacga gttcggcgac tggcgaggcg tcgaccgctg gcccgagaaa
     4681 gaagctcgtg agcgcgcgtt ccggatcttc gatcgcctgg cgaacgctgg gacactcgac
     4741 ctcggcgcca acgcgcccga ggaagatctc ccgttcctcc gcttcgctcc cgacgctcag
     4801 ggcctcttca tcgaatggcg gcacgacctc gagcgccgca tccgttcgga ggaggtcaag
     4861 gctgcacctg cctttgaggc gcacctggca aagtatcgga gcctcttccc gaagctcgcc
     4921 ctcatcttcc acctggtcga cgtcgccgac ggggcggggc cgggcgctgt gacgctgaag
     4981 gccgcgcagc tcgccgcagc gtggacggag ttcctcgagt cgcacgcccg gaaggtctac
     5041 gccgaggagc ttgccggagg cgtctccgct gctcacgcgc tcgccgagaa gatcacgcgc
     5101 gggaaggtga ccgacgggat ggccgtccgc gacgtcgccc gccgcgaatg gtccgggctc
     5161 cggacctccg ggcgcgtctt cgaagccgcc gagacgctcg aggggctcgg gtggctccgt
     5221 gtcgagagcg tcgacacggg ggggcgcccc tccgacgtca tccgccttca cgagaagttt
     5281 cggggaatcg acccagagga ggaggcgtcg tgagcgctgg attccgatcg cgttggctcg
     5341 ggtggacccc caagtcttcc gagatgaccc ctactcccac accgacaaaa ccgacagaag
     5401 cccctttcgg cagtttcggc ggttcccacc ctacccgcat cctgaagccc gcgcccccca
     5461 cgtgcccctc ttgcggcggc cccgtggagg aggacccggg agatgtcctc tgtggcgcct
     5521 gctacgccgc ccgccgcggc ccgggggagg tcctcgcgtt cgatccggac cgccgcagcc
     5581 ggaccctcgc ccgactggcc gaccgcccct gccccgactg ccaggcggtc acctggcacg
     5641 tcaacggccg gggagacgcg acctgccaga cgtgcgcgag gaggcgcgcc ggtttcgacg
     5701 agatgagcgc gcgggaggcg cgcccgtgag cccctggcct cggttcgagg agcgaaccaa
     5761 gctggttccg gaatcccgcg cgcacgggat gacggccggc gctccgatgc ccagcgtagg
     5821 gcctcggagg atgcagagat gaggcgtcgc cccaagcgaa ccctgcagac cctcccggcc
     5881 gcacgagcgg acagccgggc cgaggaggag gggctcgcga cccggcagga ggatccggag
     5941 gaggacgccc ggatccaggc gacggcagac aagctcctcg agacgaacac ccgagaggag
     6001 ctccttcatg tgatctcgac acttcgcagc gcacgaggcc cgcgcttcat gaagcatccg
     6061 aagcgggagg gcgacgtcgc attccacggc acgcccgaag agcaccaggc gagcctcatc
     6121 gaagccacgg gttgctccga cctcgagacg gcagactggc ttctccgcga agtcgcttcg
     6181 accttcctga acagcatgag tgcccgggag cgcctaaacg cagctggaag aatgctcgcg
     6241 gcactagctc caaggtcgcc gctcgaaggg ctcctctgct cccagatagt cgcggtccac
     6301 ggacttgcga tgcgactcat caacatcggg gccgcttccg agatcgggtt cgtccatctg
     6361 agtccgtcgt tggacggcgc caacaagctc ctccgcacat tcaccatgca ggtcgaggcc
     6421 ctccagcgcc tgcggacggc cggcgtgacc gagcagcgag tcgtcgtcca gcacgtgacc
     6481 gtcgccgacg gcggccaggc ggtcatcggg gcggtcagtc gggccggcgg ggaggagggg
     6541 gggcgatgac cgaatggaga aaccccatgc gatgtggggc gaagacccga ggggatcgcg
     6601 ccggccggac gtgccgcgcc ttccctgtac gcggaggaca ccgctgccga atgcacgggg
     6661 gaaaaggctc cggccggtcg cccacccacg gccgaaggac gaagcgagcc gaggtggagc
     6721 gccgggaaac ccggcgtctc atcgccgagt cgcggaggac gatcgcgctc gtcgggcgcg
     6781 aaatcggccg gagtgcatgc cggagcgttc ccaagagagt cgaatatgcc gccattccgc
     6841 cgcccgaagc ggcgcactcg gacgccaagt gtccgcgtga aggcgtcacc gttctgggta
     6901 tggcctcgaa ccccggccgc cgcaccagag ctgaacacat attgcgggag tggcgtactc
     6961 tcctcttgcc agcctcgccg agcagcgacc acgaggctcc gcgaagcagc atcgcgagcg
     7021 aatccgaagc gccggggacg agtgaggttc ctgcgacgtc actagtcctt agccaaacgg
     7081 gggagtgagt gagatgaagc agatcgctgt tgtgatgatg ctcgcgtcga tcaccggcct
     7141 tacgacgacg agccggcagg tcgtcgccga cgaaccagtc tctcggacgc ccctcgcgcc
     7201 agcggggcac agcgaacccg aacatcgctc cgacgccaga ctggctctgt tccgatcaga
     7261 accagcctca caggttctcg tggtcgagct ccccgacgcc cggaccgcac ttccggacga
     7321 gatcgtcgct ctccaggacg aggctcgcgc cggccgaacg cccacgcgga acggctttcg
     7381 ccgcgagcta ccgcagggcc tcgacgtcga cctcgccgcg cctccagcgg cccagtcttc
     7441 cggcgtcgtt cgccccggac tcctcgccac cctggagaac gggtccgtag cgtgggccgg
     7501 gtcattccgc gccagaggag catgggcgct ccgaatccag ctcacgaacg tctcagttgc
     7561 gcctggagcc cgtttcctcg tctgcggtac gacccagtct gactgcacag ccttcggaat
     7621 cgaggccgtg acggaccgag gcgagatctg gtcaccgatc gtgtccggcg acctgcttca
     7681 tctcgaggtc accgttcctg ctcctggctc cggggcgtcc ttcgggggat tcgtcccctc
     7741 cgccctcatg gaggtactcg cacccgacgt gctcctcccc gaagcacggg aggagccccg
     7801 catcatcggg gcactcgaca catcttgcat gatgaaaggc gagtgcgtct ccgacagcac
     7861 cctcgccgac ctctcaaagt atcggagcgc ctacgcgcat ctccagttcg ttgaagacgg
     7921 cgccaactac atctgttccg gagccctcct caacgacgac ggagtcgatg gattcatacc
     7981 ctacctgctg acggcgaacc actgtttcgc gaaacaggcg tctgcctcat cccttgtggc
     8041 gtactttgac taccgctacg ccagctgctc cgcgagcgcg ccgacgacac tctcaggctt
     8101 ccccaaggtc tacggggcat ccctcctcgc gacgggcgca acgagcgact tcacgtttct
     8161 caggctctcc gggcaggcgt ccgggtacag gtactacctg ggatgggatc cccggacgat
     8221 cccaagcagt tccatgatct accgtctctc gcatcccagc ggcctgccgc agcacttcac
     8281 ggcgtcgcaa ttcaccccga gcccgtcgac gtactgctcc gccctgccta cgagccggtt
     8341 cgcatattcg atggcgacca cgggaggcac tgcgggcggc agctccggag caccagcgct
     8401 ttcgaccggc ggctatatcg ttggccagct ctacggcgtc tgtggatccg gcgatctgaa
     8461 ggacccctgc gacccccgga acttcgacgt cgacggctcc ttttcgagca cgtggccatc
     8521 ggtttctcaa tggcttcggc cgacgtccgg cgcctcgcgg cctgtgcccg actttgggta
     8581 ctcgccttcg tcgcccagag tcggcgaatc cattcagttc accgacttct cgaagaacgg
     8641 cccgaccgcg tggacctggt cgttcggcga tggttcccag tcgaccgcga agaacccctc
     8701 ccatgcctac tccgccgccg gcacctatac cgtgaccctg acagccggaa acacggccgg
     8761 cgcggcctcc acatccagca ccgtcaccat ctcttccacg cccttgcagc ccacgatctc
     8821 ctacttcgga gcgaatccgc ctgcgatcgc ggcaggccag accacgacga tcacctggac
     8881 gagcacaggc ggtacgagcg ccttcatcaa ccagggcgtc ggccaggtcc cgacgagcgg
     8941 caatcgcaca atcgttcccg tcacgggtgc gccctatacc ctgactgtta gcggcccagg
     9001 ggggacggcg caggcgacac tgacgatttc gtccgtcggt acgacctacg gcaacgcctg
     9061 gatcgtcccg tccactgcgc gcgttcacgg cgacggaggg gcgttctgga cgacagacgt
     9121 cacggtcatg aaccccgagt cgaccgctgt cggcgtaaac ctcaagttcc tcggccacgg
     9181 cgaaagcgga ctctccggtc ccgaaagcac gttcaccctc gcgccgaggt cctcgcgcgt
     9241 cattcaggac gtgcttctcg cggagttcgg cctctcttcg gactggggtc cgatattgat
     9301 ccggaccgtc accggaaacg tcgtcgtgca ggcccagacc tcgacgccgg ggaatggcgg
     9361 tacctacggc cagagcgtcc cggctatctc ccgctcgagc gcgataggca cgtctccccg
     9421 ggccatctgt ggcatccgtc aggacgccgg ctaccgaacc aacatcgttc tcgtgaacct
     9481 cgcggagcag gcggcgcaga tcgacctcgt cctgctctcc cctaccggct ccgtacttgc
     9541 gtccatacag gagacgctcg gaccgctcgg cttccgccag ctcaacgtgg cgaacgatct
     9601 acggatctcg aatctcgctg gaggcaccct cgtgctctcg agcaagtcct ccctcacgcc
     9661 cttcgctgcc tatgcatccg tcatcgacaa ccgcaccgcg gatccgcgga ctctcctgcc
     9721 gcagtgacga acaactcgct gccttctgac atgcgccgct cgctgcaggc tccgcgcgcc
     9781 cacaagcgtc tgtgggtacc tctcttgacg ctcctcctgg ccctcccagt tccttcgcga
     9841 gcccagtcgc tgatcgacct cgagtacacc ccgagagccg ccgacgagga catccgtcag
     9901 gccgtcgagc tgctggacga cgacggtccg attgcgttct ccctgcgctt tgccagggag
     9961 ggcgctccag ctccgggatc ggaggagatc gggcgcatcc gggtcgggac accggctgtc
    10021 cgcctctacg ccaggcagtc cgtcgtctcc tttctccagg gggctgccac gacacagttc
    10081 aaggactggg gccatccgct cgctgacaac gccggcgtcc gcgtagaggc caacgtgcgt
    10141 cggttcttcg tcgacgagag ctacgtctac aaggccgaga tcgcgatcgg ctacgtcgtc
    10201 cgcggtcctg acggaagtgt gctctggaac gccgagatcg attccgatgc caaggtcttc
    10261 ggcgtggccc ggaaggcgcc gaactaccgg gaggctctca gcgacgcctt tcggaagaac
    10321 ctggcgctcc tgctcgcacg accggggttc cgggcggcgt gcacgggtaa gcggggctca
    10381 aaggccgaca ccgcaggccc gaccgtcacc cccgccgagg cgaggctagc cgttctgcga
    10441 ttgatcaagg agggccttgg cgacgacctc ctcgtcgcgt acgtccgcca ggcccggatc
    10501 agtccggcct tctctgctga cgacattctc gagtggaaga gcgcggggat tccagcctcg
    10561 gtcatcacgg cggcgctcgg tgccgcagcg ccggcccctg agccgtagca ggccctgcgt
    10621 cgacgctctg aacccgatct ccgcgcacct gcgctggggc gtcgtcactg ccgacgaccc
    10681 ccagaccagg gttcgcagcg gccagggtgt tcggccgttg tcggaggttc tgcgctgctc
    10741 tacgctcaac gcctcatggc cagcgaactc gcacgcgaac cgcagacgtc cccccccacc
    10801 gtcgctctcg agccgtggtg cacagggctg ttcttccgct tcgcgctctt ccggctccac
    10861 gttcggccgt ggctcctcgg ccttctcgct ttcgctggct actgggggct tccctccctg
    10921 ttcgcgacct tctccgaaac tctagtgacg cgcgaggcca tcgcaacgat catttccggg
    10981 ctccccccct ttgtccagca gctcggctgg caccaggccc tgctcgacgg gcagaggatc
    11041 caggaagggc tcgcctactt gaacgaccgg tctcatttcg tcttcgcggc cctgctcctg
    11101 cccggcgcgg cagtcgccgc gctgatcctc gcgcgcgtcc acgacacggt cgcagccctc
    11161 cgtgacgacg gcatcctccg ggacgacgac gccaccatcc gggagaccta ccagcgcttt
    11221 caccggctcg caaaccactg gttcttccga ctcgccgccc tgtgcctcgg cggagtcacg
    11281 gcgctcgtgt tcctggcctt tcgcaacggc gacgcccata cgtcctggtg gggacaccgc
    11341 cagttcggac ccggagggct ggcgttcgcc ttcatcgagg ccgctatggt gtacttcggc
    11401 acccaaacga tggtcttgac ggccttcgcg tcggcgatgt tcgcgaggct ccttgcgggc
    11461 cggatcgcct ttcgtccctt ccacccggat ggcgccaacg gcctcgctcc gatgggccgg
    11521 atgatcatga tgctctggat cctcgcgctc gtcctgggcg cggagatatg cgtcgcactc
    11581 ttcctcggct atctcggcat cgagaagatg ccgttgacgt ggctgctcgc cctggcgggc
    11641 ctcgcggcta tcccagcgct cgcgatcgcg ccgctctacg cgtcgctcca cgccatccat
    11701 cgggcccgca tcgcacggat cagccacctc gagccgctcc tcaattccat gctcgagcgg
    11761 gccgaggggc aggtcgacga gggccgcttc gacgacgcct ccgaaaccct cgccacgctg
    11821 ggcgatatgc agggcgtgca cgagtccttc gcttcgctca acgtgtggcc cttcaacccc
    11881 cgcgcgctcg cgggtgtcgt ggcggtctac gcgctccagc tcgtgctcac gctcaaggag
    11941 gttctgggct tctgagactc ctgcgccgca ggatccaagg gccgacagcc tccgcgagca
    12001 gccacatcgg gtggaagagc acgacgtagc cgcagccgac gaggatcacc gaccaccacg
    12061 gcttcggcat cagcgccgcg gcgccccagg cccataccgg ccagagcgca ttggccgcct
    12121 cgtaaacgac gccgagggcc acgctcgaga gccacaaacg cgccgccggc cggaagcggc
    12181 ccgccgcgac cgcagcgaaa ggcccctcgc ggcccaggag gagcgagagg ccctcccccg
    12241 cgagaacgac tgccccgccg atcaggaaca cggcggcgag gaggccgggg tccgcgacga
    12301 gcaccatggc caccagtccg cccgctagcg agactgcgag aaggcccgcg ggtcgggagc
    12361 gcttcggcgg gccggaggcc gccggcctcc acagatgtat gaccgtggcg atcgcgaggc
    12421 cgtacgagag cgctccgttg gcgacgagaa aggctggcgt tagcgggagc cggtagccaa
    12481 agacatgcct ttcggcgccg atcacgaggt cgaaaccgag accgagcggt acaccgacag
    12541 acgcccccgc cacgagggca ccgacgatct cgcggcgcgg cgcctgagag aggtacccga
    12601 ccacggccca agccacgctc tggaccgcga cgacggcgag aaaggcgatc acgtcgggag
    12661 ccgtgcggga acctctcact gcagatccta cgcgaggacc cggcctggca acggacgaag
    12721 gggggggtgg gaggtgagcg tgacgtgacg agaccccctc gtaccgggcc acgcgccttt
    12781 ctcggtcgcc ttcattccga gcacccggga catgcgccga tgatctcgcc accctccagc
    12841 tcgagtaggg tccggccgaa cagctcaccc gtcagctcag cgattccggc ctgtctcttc
    12901 agcccgcagc cctcggccac ggcgatgtac tgccgcgccg tcttcgcgtc cactcgacct
    12961 tcgcgccaac ccttctgcag gcgatgggtg agcgtccgac gaacctcgtt cttcccggtc
    13021 cgcgtgtgac cgccgattct cggtggcgcg gagtggccga attcgcggcc aggaatcatc
    13081 cgaatggaat cgggtcg
//
LOCUS       DAOONL010000091        12982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5296_length_12982_cov_17.528584, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000091 DAOONL010000000
VERSION     DAOONL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_12982_cov_17.528584"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLP50_12555"
     CDS             complement(<1..403)
                     /locus_tag="PLP50_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPA52420.1"
                     /translation="MELRKEQRRMKYEGKAIQTVPLDGGFVELRFDLQGDSVNKFNSL
                     TLGELKEVVAGLVAAPPKGLLITSGKDVFIVGADVTEFLTHFRRSEEELAGWLLETDA
                     TFSAIEDLPCPSVVAINGFCLGGGFELALSAH"
     gene            593..1309
                     /locus_tag="PLP50_12560"
     CDS             593..1309
                     /locus_tag="PLP50_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPA52421.1"
                     /translation="MSFVSSEEKTLAFVAAQLGASPKGPPRSREKRRPLVTVSRETGA
                     GGNTFGEALCGWLEENQPKGRGPWVLVDRELVDKILEDDALPDRLASWSPEDHLAGVS
                     FVIEELLGLHRAAWKPMQETTETILRLGEMGNVVLVGRGANVILGHLPQAFHVRLVAS
                     LESRVENVAAARELSKKAACAWVEAEDKARRRFIRRYFQVDVADPLLYNLVVNVDRVP
                     IEEAARVVGEAVLARAERGG"
     gene            complement(1347..1643)
                     /locus_tag="PLP50_12565"
     CDS             complement(1347..1643)
                     /locus_tag="PLP50_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52422.1"
                     /translation="MLARERLAKCPTCQGTGLGGPCARCSSLGRIRFQGNEEQWILCG
                     YCRGTGTVDCPRCRREGRIRFFSFQCPRCGFVGPESEGHLDELYADLCPKCGSY"
     gene            complement(1730..3976)
                     /locus_tag="PLP50_12570"
     CDS             complement(1730..3976)
                     /locus_tag="PLP50_12570"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPA52423.1"
                     /translation="MKARGSLLLLAGLAVVAGPALCLQEGADGLRGAEIRISRAAGPI
                     TVDGDLGDAGWLGAGRVETFYETNPGDNLPPKVRTVGRLAYDGRHLYAALECDDPEPG
                     KIRAPFAQRDVLGGGTDYAGIILDTRNDGRTAILFLANPRGVQYDAVTDDGNEDSSPD
                     FHWEAAAKITPTGWNLEIRIPFSSLRYDRADPQTWGILLYRNRPRDFRYQMFSARLPR
                     GRNCFICSENVLTGLEGLPPGGHLVVAPYATASEETLPRADGTGSVARPVEPDGGLDV
                     KWTPSAGVALDGTFNPDFSQIESDTARIGVNERFALFYPEKRPFFLEGVDLLSTPVQA
                     VYTRSVTSPRWGLRGTGKRGGTSWTALVAEDRGGGSVILPGPNGSGLAAQDFRSLDFV
                     GRVRRDVGRSFASFLVTAKEMPGSASNRVLGPDFRLNLSETDSVTGQLLWSWSETPDR
                     PERAAEWDGRSLSGHGADVWWSHTTETWDWGLEYKDFSDGFRADLGFVPQVGFREGYG
                     ELGRTFRPTGFVSRVRVFAFADSLADREGELLSQVVASAVRLETKYNGSVLLELRNER
                     IRAGTELLSRRRLVYGVGAVPSRFFAGIAIDGAIGEEIDFEGSRTGKGGWLAASVVFR
                     PGDHLRLDLSGEVRWMDVDLPDGRSPRLFTAHVERMNATWTLTARAWVRVIGQYVGAW
                     QAPELYPTDVEAESGSFSASALLGYRLNWQSVVFLGYGDARTLSEDGGLPPESRQLFL
                     KVSYAFQR"
     gene            complement(4054..5691)
                     /locus_tag="PLP50_12575"
     CDS             complement(4054..5691)
                     /locus_tag="PLP50_12575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPA52424.1"
                     /translation="MRKPAALLVLAATLALAAPDAPACTNILVTKGASADGSTMITYA
                     ADSHELYGELYFTPAGVHTFGAMREIVEWDTGKTLGRIPQAPVTYQVVGNVNEYQVSI
                     GETTFGGRKELEGPAGIIDYGSLMWIGLERSKTAREAIEVMTKLVDEFGYASSGESIS
                     IADPNEAWVLEIIGKGEKQKGAVWVAVKVPDGTISAHANQARIRQFPRNDPKNVLFSK
                     DVVSFARDKGWFTGKDEEFSFADAYAPLRASTLRSCDGRVWSVFRRAAPSLNLSSDWI
                     LAVPGAKPMPWAIKPDKKLTTKDVMELMRDHFEGTELDMTKDIGAGPFVLPYRWRPMG
                     FKVDGVDYVHERAISTQQTGYSFVAQLRSYLPNPVGGVLWFGVDDTYSTVYTPMYCGI
                     REVPKSFAVGTADFSAFSWDSAFWVFNFVTNWAYSRYSDMIVDVRAAQDELEGRFLAE
                     QPEVEKEAVRLHAASPAAARDYLTKYSVGAGDLVFARWKKLGDHLLWKYMDGNVRDTQ
                     GGVTHPKYREEWYRAIAKDGGDRLKARKLAEEPKRPE"
     gene            complement(5821..6093)
                     /locus_tag="PLP50_12580"
     CDS             complement(5821..6093)
                     /locus_tag="PLP50_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPA52425.1"
                     /translation="MHWKTFATVFVTVLVAEMGDKTQLATLLFSADRQTSKWVVFAGA
                     ALALTAAAGIGVLVGSQLERFLSPKLLKTIAGVGFLAIGAWTLLSK"
     gene            6197..6820
                     /locus_tag="PLP50_12585"
     CDS             6197..6820
                     /locus_tag="PLP50_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52426.1"
                     /translation="MGPAHRPSAILAALLGAALAAGAVPAARAADPPKSGVISRPKGF
                     STIRLVAPPGLSVFLGDVPVGTTDVLRQGLWLQDLPAGRYVLRVEKAGFESKEVTVLV
                     GEGDTKEVRVLALRPRPTPTPAPPETAAAASPAPSAPSPAPVRAVILSRTPVAGGTLV
                     TGIASSAVDALPLIEDLKSECRCTPALESMTKRKDGLYEFRVRLPGT"
     gene            6856..9573
                     /locus_tag="PLP50_12590"
     CDS             6856..9573
                     /locus_tag="PLP50_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA52427.1"
                     /translation="MDSRNTDTSGLEREPLRPLPPPVPGDVLSGRWELRRLLGQGGSG
                     LVFEAWDRELKGAVALKLLRPDRLSPASLARLRREVKVAHAVSSPRLVRVYDLGEDHG
                     RPFLTMELVPGAPLRDRTSGPLPIGDVVRIGIEVLEGLAALHAAGIVHRDVKPGNVLL
                     GPDGGARIVDFGLARSLDRDETRLTETETALGTADYMAPEQALGHDVDARSDLYAFGV
                     LLFELLTGDLPWRAESSFGSVLARLRAKAPDVKKLRPETPRWLAETVGRLLERKPADR
                     HESAEAVLAVLRRESVGWREVLARRRRPLLAVAAALAVALLGAAGWRLAAKPSLASVA
                     ADGAFGARGVDRSGRILWRRPDLDPARCAAAGRFRKDGPVEVAAILEREAYERAPERV
                     YTLSFLEAATGRVARTAALAPELNGFPVNERRFAVTTMKAADTDGDGLDEVFVSYFHS
                     WAPSFVLMWNPADGRSRMTFTGGGHHRLVDAADVDGDRRKEMLLLGINSPFGWYRAFA
                     VARLWERDPRDASPRITPFGTFSADQAGAGSDSNLIDYVLLPRGIFSEDSRELRAVDG
                     TSFALDYGNGRTVRIDLGGCGLLSPSESLDCRALRRSRMSAYANLREGLRRGRAGVAD
                     EAVESFDAAERDARSARDPILLELVHRWRGMTLLLHGNAREGAAVLAELVRVSENALE
                     IADDAGRELHKAGRLPEAIAWYSRALERAGPEHTGRSVTYPREGLLLAFAELGRWEEA
                     SRWVDRWSAAASTRVVARVPWYRAFLAWRQGIAFDPPELTLGTYDLLHLWWYEMLLAR
                     NAEPKPLLEQARAEFVEAVRARGPLLSVQAELLDRLGRREEARAVAREAWRETEAAVR
                     TEVWTRAHLDLVVERVVRLAGGDEARHARALLETVRAEAKRPDPGTKTR"
     gene            9614..11455
                     /locus_tag="PLP50_12595"
     CDS             9614..11455
                     /locus_tag="PLP50_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52428.1"
                     /translation="MLRLVIRYRHEALVFPFPVAEVTLGSSSKADLVVPFPGVSRLHA
                     RISPEADGIRIVDAGSKNGVIRDGQRTLRAGLREGDEVLLGDATLALQEAGEAEDLAL
                     ELGALKRGAAPPTGSTASGPLDAPAAALRLVRELEGDGRETPTRRRALLRKARTILGA
                     QGLVALDSLAASEPAFLDVDGPAPDKALIETLRAVVGKRTSSRAPLPLFLAGGQSVAL
                     APATSSLQGPCLIALFSELPGAEVSWKRELLAFLASRLLSPTEDADDPSPPAGARSLR
                     LPEALVLGSSPAFASFLATLEATVGSGLDALLTGETGTGKELIARAIHDSGPHPDGPF
                     VAINCAAIPNELLEAELFGVTRGAATGVEARPGRFLQANGGTLFLDEIGELPEPLQAK
                     LLRVVQEREVLSLGGASARRIHVRIVAATNRDLLRRMKEGLFRPDLYYRLSALQYHVP
                     PLRDRREDIPALVLAFASRSAASLGRRVRGVSRPALDVLLSHDWPGNVRELETAVQRA
                     VLLCPSGGTLLAEHFSQVRWAVEERAREAVVLTPVDVLGAPQPCSPPPFAVPGDLDLE
                     ARRNAVEKETIERALATAKGNKSLTARLLRISRNGLAARMKALGIGE"
     gene            11744..12472
                     /locus_tag="PLP50_12600"
     CDS             11744..12472
                     /locus_tag="PLP50_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52429.1"
                     /translation="MRNAISRRIALVLSLAPLSFPVQAQVRGGRAQGAIVVSESANVY
                     RNSDGDAVEAVVSRGDFVGSITGSELLSAQYLLDEVNGRVHVMYFKNKEQKGTMFTAW
                     MNPADLASFFYECGCGPEGKECAPYQLKFMSRRWNACYVEARDKKLAELKEKWASGGG
                     GETNSSPTAPPAKTASADRPLSNADVINMTTAGLDEALIISKIEKASTEALDSSPDGL
                     VALKQAGATKAVIAAVSRRVEMRQ"
     gene            complement(12722..>12982)
                     /locus_tag="PLP50_12605"
     CDS             complement(12722..>12982)
                     /locus_tag="PLP50_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA52430.1"
                     /translation="ATRRWLAAHEDRIVVHFLLAYAPETNPDEHVWSQLKGLFRRTPV
                     FATEDFDAAVENAMTGIALDRSLVRCFFDHPDVAYVKAALHW"
BASE COUNT         1874 a         4776 c         4389 g         1943 t
ORIGIN      
        1 agtgggccga gagcgcgagc tcgaagccgc cgccgaggca gaagccgttg atcgccacca
       61 cggaggggca cgggaggtcc tcgatcgcgc tgaacgtcgc gtcggtctcg aggagccagc
      121 cggcgagctc ctcctcgctt cgcctgaagt gcgtgaggaa ctcggtcacg tccgcgccga
      181 cgatgaagac gtccttgccg ctcgtgatca ggagtccctt cggcggcgcg gccacgaggc
      241 ccgcgacgac ctccttcagc tcgccgaggg tgaggctgtt gaacttgttg acggagtcgc
      301 cctgcaggtc gaaccgcagc tcgacgaagc ctccgtccag cggaacggtc tggatggcct
      361 tcccttcgta cttcatccgc ctctgctcct tcctcagctc catccgggaa tccggtcggg
      421 ttcggtccgc gatcatatcc gccgcgccgc agcacaggag ccgaggccag ggagctgggg
      481 tcaggtcttg tattgtgact ttcgctcccg cgaaagtcac aatacaagac ctgaccccag
      541 gtgtttccgc ggcgtagact gggtgcagct ccgcttcgga aggaggatcg ccatgtcctt
      601 cgtctcgtcc gaagagaaga ccctcgcctt cgtcgccgcg cagctggggg cctcgccgaa
      661 ggggccgccg cgctcgcgtg agaagagacg ccccctcgtg accgtctcgc gcgagaccgg
      721 ggcgggcgga aacacgttcg gcgaggcgct ctgcggctgg ctcgaggaga accagccgaa
      781 ggggcgcggc ccgtgggtcc tggtcgaccg cgagctcgtc gacaagatcc tggaggacga
      841 cgccctcccg gaccggctcg cctcctggtc gccggaggac cacctcgcgg gagtctcgtt
      901 cgtgatcgag gagctcctcg gcctccaccg cgcggcctgg aagccgatgc aggagacgac
      961 ggagacgatc ctccggctcg gagagatggg gaacgtcgtc ctcgtcgggc gcggcgcgaa
     1021 cgtcatcctc gggcacctgc ctcaggcgtt ccacgtccgc ctcgtcgcgt cgctcgagag
     1081 ccgcgtcgag aacgtcgccg cggcccggga gctttcgaag aaggcggcct gcgcctgggt
     1141 cgaggccgag gacaaggccc ggcggcgctt catccgccgc tacttccagg tcgacgtcgc
     1201 cgacccgctc ctctacaacc tcgtcgtcaa cgtcgaccgc gtcccgatcg aggaagccgc
     1261 gcgggtcgtg ggcgaggcgg tcctggctcg cgccgagcgg ggagggtgag gccggcgcaa
     1321 cgtcccgctt cgccgctcgc gcgacatcag tagctcccgc acttcgggca gaggtcggcg
     1381 tagagctcgt cgaggtgccc ctccgactcg ggccccacga agccgcagcg ggggcactgg
     1441 aacgagaaga agcggatgcg accctcccgg cggcagcgcg gacagtcgac ggtgcccgtc
     1501 ccccggcagt agccgcagag gatccattgc tcctcgttcc cctggaagcg gattcggccg
     1561 agcgagctgc agcgcgcgca ggggcctccg aggccggtcc cctggcaggt cgggcacttc
     1621 gcgagtcgtt cgcgcgcgag catgggaacc tcccggccgt cgaggccgcg gctttcgttc
     1681 gtgcgtgcgg agatcgctgg agaaagtcta cgctccggga gcgctccgat caccgctgaa
     1741 acgcgtacga gaccttcagg aagagctggc ggctctccgg agggagcccg ccgtcctcgg
     1801 agagcgtccg ggcgtcgccg tagccgagga agacgacgct ctgccagttc aggcggtacc
     1861 cgaggagggc cgacgcggag aacgagcccg attccgcctc aacgtccgtc gggtagagct
     1921 ccggcgcctg ccaggctccc acgtactgcc cgatgacccg gacccaggcg cgcgccgtga
     1981 gggtccacgt cgcgttcatc ctctcgacgt gcgccgtgaa gagccgcggt gagcgcccgt
     2041 ccgggaggtc gacgtccatc cagcggacct cgccgctgag atcgagccgg aggtggtcgc
     2101 ccgggcggaa gacgacgctc gccgcgagcc atcctccctt ccccgtgcgc gacccctcga
     2161 agtcgatctc ctcgccgatc gccccgtcga tcgcgatccc ggcgaagaag cgggagggaa
     2221 cggcgcccac gccgtagacg agccgccttc gcgacaggag ctcggtcccg gcccggatcc
     2281 tctcgttccg aagctcgagg aggacggagc cgttgtactt cgtctcgagc cggaccgcgg
     2341 acgcgacgac ctggctcagc agctccccct cccggtcggc gagggagtcg gcgaaagcga
     2401 agacccggac ccgtgagacg aagccggtcg gccggaaggt cctcccgagc tcgccgtacc
     2461 cctcccggaa gccgacctgc gggacgaagc cgaggtcggc ccggaagccg tcggagaagt
     2521 ccttgtactc gagcccccag tcccacgtct cggtcgtgtg cgaccaccag acgtcggccc
     2581 cgtggcccga gagggagcgc ccgtcccact ccgccgcccg ctcggggcga tccggcgtct
     2641 cgctccacga ccagaggagc tgccccgtca ccgaatcggt ctcgctcagg ttgaggcgga
     2701 agtccggccc gaggactcgg ttgctcgccg agccgggcat ctccttggcc gtgacgagga
     2761 acgacgcgaa ggagcggccg acgtcccgcc ggaccctccc gacgaagtcg agcgaccgga
     2821 agtcctgcgc cgcgagcccg gagccgttcg gaccgggaag gatgacgctc ccgcccccgc
     2881 ggtcctcggc gacgagcgcc gtccagctcg tcccgccccg cttccccgtt ccgcgaaggc
     2941 cccagcgcgg cgacgtgacg gagcgggtgt agacggcctg caccggcgtc gagaggaggt
     3001 cgactccctc gaggaagaag gggcgcttct cggggtagaa gagggcgaag cgctcgttca
     3061 cgccgatccg ggcggtgtcc gactcgatct gggagaaatc ggggttgaac gtcccgtcga
     3121 gcgccacgcc ggcggacggg gtccatttca cgtcgaggcc gccgtccggc tcgacgggcc
     3181 tcgccacgct ccccgtcccg tccgcgcggg gcaacgtctc ctccgaggcg gtcgcgtagg
     3241 gcgccacgac gagatgcccg cccgggggaa gcccttcgag gccggtcagg acgttctccg
     3301 agcagatgaa gcagttccgt ccgcgcggga gccgggcgga gaacatctga tagcggaaat
     3361 cgcgcgggcg gttcctgtag aggaggatcc cccaggtctg cgggtccgcc ctgtcgtacc
     3421 ggagagagga gaacgggatc cggatctcga ggttccagcc cgtcggcgtg atcttcgccg
     3481 ccgcttccca gtggaagtcg ggcgaggagt cctcgttccc gtcgtccgtc acggcgtcgt
     3541 actggacgcc gcgcgggttc gcgaggaaga ggatcgccgt ccggccgtcg ttgcgggtgt
     3601 cgaggatgat cccggcgtag tcggtcccgc ccccgaggac gtcgcgctgg gcgaacggcg
     3661 cccggatctt cccgggttcg gggtcgtcgc actccagggc ggcatagagg tgccggccgt
     3721 cgtaggcgag gcgcccgacc gtccggacct tcggcgggag gttgtccccc gggttcgtct
     3781 cgtagaaggt ctcgacccgc cccgccccga gccagccggc gtcgccgagg tcgccgtcga
     3841 ccgtgatcgg accggcggcg cgcgagatcc ggatctcggc gccgcgcagc ccgtccgccc
     3901 cctcctgaag acaaagcgcc ggaccggcca cgacggccag cccggcgagg aggaggaggg
     3961 aacctcgggc tttcacccgc cctctgatac ggacggcggg ggcgcggggt ttcggcccgc
     4021 gccccacctt cccggccgat ccggctcttc cggctactcc ggccgcttcg gctcctcggc
     4081 gagcttccgc gccttcagcc ggtcgccccc gtccttcgcg atcgcccggt accactcctc
     4141 gcggtacttc gggtgggtga cgccgccctg cgtgtcgcgg acgttgccgt ccatgtactt
     4201 ccagaggagg tggtcgccga gcttcttcca gcgggcgaag accaggtcgc ccgccccgac
     4261 ggagtacttc gtcaggtagt cccgcgccgc cgccggcgag gcggcgtgga ggcgcacggc
     4321 ctccttctcg acctcgggct gctcggcgag gaagcgtccc tcgagctcgt cctgcgccgc
     4381 gcggacgtcg acgatcatgt cgctgtagcg cgagtaggcc cagttcgtga cgaagttgaa
     4441 gacccagaag gccgagtccc acgagaaggc cgagaagtcg gccgtcccga cggcgaagct
     4501 cttcggcacc tcgcggatgc cgcagtacat cggcgtgtag acggtggagt aggtgtcgtc
     4561 gacgccgaac cagaggacgc cgccgaccgg gttcgggagg tacgaccgga gctgggcgac
     4621 gaacgagtag ccggtctgct gcgtggagat cgcccgctcg tggacgtagt cgacgccgtc
     4681 caccttgaag cccatcggcc gccagcggta cgggaggacg aacggcccgg cgccgatgtc
     4741 cttcgtcatg tcgagctcgg tcccttcgaa gtggtcccgc atcagctcca tcacgtcctt
     4801 cgtcgtcagc ttcttgtcgg gcttgatcgc ccagggcatc ggcttggcgc ccgggacggc
     4861 gaggatccag tccgacgaga ggttcagcga cggcgccgcg cggcggaaga cgctccagac
     4921 ccggccgtcg cagctccgga gcgtgctcgc gcggagcgga gcgtacgcgt cggcgaacga
     4981 gaactcctcg tccttccccg tgaaccagcc cttgtcgcgc gcgaaggaga cgacgtcctt
     5041 cgagaagagg acgttcttcg ggtcgttccg cgggaactgc cggatccggg cctggttggc
     5101 gtgggccgag atcgtcccgt ccggcacctt cacggcgacc cagacggccc ccttctgctt
     5161 ctcccccttc ccgatgatct cgaggaccca cgcctcgttc gggtcggcga tggagatcga
     5221 ctcaccgctc gaggcgtagc cgaactcgtc cacgagcttc gtcatcacct cgatcgcttc
     5281 gcgggccgtc ttcgaccgct cgaggccgat ccacatgagc gagccgtagt cgatgatccc
     5341 ggccgggcct tccagctcct ttcggccgcc gaacgtcgtc tcgccgatcg agacctggta
     5401 ctcgttcacg ttgccgacga cctggtaggt caccggcgcc tgcggaatcc ggccgagcgt
     5461 cttgccggtg tcccactcca cgatctcgcg catcgcgccg aacgtgtgga ccccggcggg
     5521 cgtgaagtag agctcgccgt agagctcgtg gctgtcggcg gcgtacgtga tcatcgtcga
     5581 gccgtcggcc gaggccccct tcgtcacgag gatgttcgtg caggcggggg cgtcgggcgc
     5641 ggccagggcg agggtcgcgg cgaggacgag gagggcggcg ggctttctca tgcggctttc
     5701 gcggctcctt agacggggcc gccctgcccg cgatcgccgc ggggagccgc cccgcgagca
     5761 tcgtaccgga ggcaggacga ccgcgcccga cacgcgccgg aacgcaggac ctcgcgggtc
     5821 ctacttcgag aggagggtcc aggccccgat cgcgaggaag ccgacgccgg cgatcgtctt
     5881 caggagcttc ggagagagga agcgctcgag ctgcgagccg acgaggacgc cgatgccggc
     5941 ggccgccgtc agcgcgaggg cggcgcccgc aaagacgacc cacttgctcg tctggcgatc
     6001 cgccgagaag aggagcgtcg ccagctgcgt cttgtcgccc atctccgcga cgaggaccgt
     6061 gacgaacacc gtcgcgaacg tcttccagtg catcggactg cctccgcccc gcgtgcgtcg
     6121 ccgcgcgccg cgcccgggac gcgggaggat accggaggcg ctcgcgcccc gcctctccgg
     6181 ccgctacgat cgccccgtgg gtcccgctca ccgcccttcg gcgatcctcg ccgcgctcct
     6241 cggcgcggcc ctggccgcgg gcgccgtccc cgcggcgcgc gcggcggacc cgccgaaatc
     6301 gggcgtcatc tcccgtccga agggcttctc cacgatccgc ctcgtcgccc cgcccggcct
     6361 ctccgtcttc ctcggcgacg tcccggtcgg gacgaccgac gtccttcgtc agggtctctg
     6421 gctgcaggac ctgcccgccg ggcgctacgt cctgcgcgtc gagaaggccg gcttcgagtc
     6481 gaaagaggtc accgtcctcg tcggcgaggg cgacacgaag gaggtgcgcg tcctcgcgct
     6541 tcgcccgagg ccgacgccca cgcccgctcc gccggagacc gcggcagccg catccccggc
     6601 cccttccgcg ccgagcccgg cgcccgtccg cgccgtgatc ctttcgcgga cgccggtcgc
     6661 cggcggcacg ctcgtgacgg gcatcgcctc gagcgccgtc gacgcgctcc cgctgatcga
     6721 ggatctgaag tcggagtgcc gctgcacgcc cgctctcgag tcgatgacga agcgaaagga
     6781 cgggctgtac gaatttcgcg tgaggctgcc gggaacgtag actacgggaa tcgccgacgg
     6841 ggcggactcg aaggcatgga ttctcgaaat accgacacct cgggcctcga gagggagccg
     6901 cttcggccgc tccctcctcc cgtgcccggc gacgtcctct cggggaggtg ggagctccgc
     6961 cgcctcctcg gccagggtgg ctccggcctc gtcttcgagg cgtgggaccg agagctgaaa
     7021 ggcgccgtcg ccctcaagct ccttcggccc gaccggctct ctcccgcttc cctcgcccgc
     7081 ctgcgccgcg aggtgaaggt cgcgcacgcg gtctcgagcc cgcgcctcgt ccgcgtctac
     7141 gacctcggcg aggaccatgg tcgtccgttc ctcacgatgg agctcgtccc gggcgcgcct
     7201 ctccgggacc ggacgagcgg accgcttccg atcggcgacg tcgtccgaat cggaatcgag
     7261 gtcctggaag ggctcgccgc ccttcacgcc gcggggatcg tccaccgcga cgtgaagccc
     7321 ggcaacgtcc tcctcggccc cgacggcggg gcccggatcg tcgatttcgg cctcgcccgg
     7381 agcctcgacc gcgacgagac gcgcctcacc gagacggaaa cggccctcgg cacggccgac
     7441 tacatggccc cggagcaggc cctcggccac gacgtcgacg cgcggtcgga cctctacgcc
     7501 ttcggcgtcc tcctcttcga gctcctcacg ggcgacctcc cgtggagggc cgagtcgtcg
     7561 ttcggctccg tcctcgcgcg gctccgcgcg aaggctcccg acgtgaagaa gctccgcccc
     7621 gagacgccgc gctggctcgc ggagaccgtc ggccgcctcc tcgagcggaa acccgcggac
     7681 cgccacgaga gcgcggaagc ggtcctcgcg gtcctccgcc gggagtcggt cggctggcgg
     7741 gaggtcctcg cccggcggag gcgcccgctc ctcgccgtcg ccgccgcgct cgctgtcgcc
     7801 cttctcggcg ccgccggctg gcggctcgcc gcgaagccct cgctcgcttc ggtcgccgcc
     7861 gacggcgcct tcggcgcgcg cggcgtcgac cgctcggggc gcatcctctg gaggcggccc
     7921 gacctcgacc ccgcgcgctg cgcggcggcg ggccgcttcc ggaaggacgg acccgtggag
     7981 gtcgcggcga tcctcgagag ggaggcttac gagagggcgc cggaacgggt ctacacgctc
     8041 tccttcctcg aggccgcgac cgggcgcgtg gcccgcacgg cggccctcgc gcccgagctg
     8101 aacggcttcc ccgtgaacga gcggcgcttc gccgtcacga cgatgaaggc cgccgacaca
     8161 gacggtgacg gcctcgacga ggtcttcgtc agctacttcc actcctgggc gccgtccttc
     8221 gtcctgatgt ggaaccccgc ggacgggcgg tctcgcatga cgttcaccgg cggcgggcac
     8281 catcgcctcg tcgacgcggc cgacgtcgac ggcgaccggc ggaaggagat gctcctcctc
     8341 ggcatcaaca gcccgttcgg ctggtaccgc gccttcgccg tcgcgcggct ctgggaacgc
     8401 gacccgcgag acgcctcgcc gcggatcacc cccttcggca ccttctcggc cgaccaggcg
     8461 ggcgcaggca gcgactcgaa cctgatcgac tacgtgctgc tcccgcgggg catcttcagc
     8521 gaggactccc gcgagctgcg cgccgtcgac ggcacgtcct tcgcgctcga ctacggaaac
     8581 ggccgaaccg tccgcatcga cctcggaggc tgcggtcttc tctctccgtc cgagagcctc
     8641 gattgccggg ctctgcgacg aagccggatg tcggcctacg cgaacctgcg cgagggcctg
     8701 cgacgcggcc gggccggcgt cgccgacgag gccgtcgagt ccttcgacgc cgccgagcgc
     8761 gacgcgcgct ccgcccgcga cccgatcctc ctcgagctcg tccaccgctg gcgcgggatg
     8821 acactgctcc ttcacgggaa cgcccgggag ggggccgccg tcctcgccga gctcgtccgc
     8881 gtctcggaga acgccctcga gatcgccgac gacgccggcc gcgagctcca caaggcgggc
     8941 cggctccccg aggcgatcgc ctggtactcc cgcgccctcg agcgcgccgg ccccgagcac
     9001 accggacgct ccgtgaccta cccgcgcgaa gggctcctcc tcgccttcgc ggagctcgga
     9061 cgctgggagg aggcgtcccg atgggtcgac cgatggtccg ctgcggccag cacgcgcgtc
     9121 gtcgcccgcg tcccgtggta tcgggcgttc ctcgcctggc gacagggcat cgccttcgac
     9181 ccgccggagc tcaccctcgg gacgtacgat ctcctccacc tctggtggta cgagatgctc
     9241 ctcgcgagga acgccgagcc gaaacccctc ctcgagcagg cccgggccga gttcgtcgag
     9301 gccgtccgcg cccgcgggcc gctcctctcc gtccaggccg agctcctcga ccggctcgga
     9361 cgccgcgaag aggcacgagc cgtcgcccgc gaagcctggc gcgagaccga ggcggccgtc
     9421 cggacggagg tctggacccg cgcccacctc gacctcgtcg tcgagcgcgt cgtccgcctc
     9481 gccggcggcg acgaggcccg ccatgcccgc gccctcctcg agacggtccg cgccgaggcg
     9541 aagcggccgg acccggggac gaagacgcga tgagcggggc acggcgtttg ctggttgccg
     9601 atcgaggtgc tcgatgctga ggctcgtcat ccgctaccgc cacgaggccc tcgtctttcc
     9661 gtttcctgta gcggaggtca ccctcggctc ctcttcgaag gccgacctcg tcgtgccgtt
     9721 ccccggcgtc tcgcgcctcc acgcccggat ctcgccggag gccgacggca tccgcatcgt
     9781 cgacgcgggc tcgaagaacg gcgtcatccg ggacgggcag cgaacccttc gcgcagggct
     9841 tcgcgagggc gatgaggtcc ttctcggcga tgcgacgctg gccctccagg aggccggcga
     9901 ggctgaggat ctcgccctgg agctcggcgc gttgaagcgt ggcgcagcgc ccccgaccgg
     9961 ctcaacggcg agcggtccgc tcgacgctcc cgccgcagcg ctgcgcctcg tgcgagagct
    10021 ggaaggagac ggtcgcgaga caccgactcg gcggcgagcg ctgctgcgca aggcccgaac
    10081 gatcctgggc gcccaaggtc tcgtcgcgct cgactcgctc gctgcttcgg agccggcctt
    10141 ccttgacgtg gacggcccgg cgcccgataa ggccctcatc gaaaccctgc gcgccgttgt
    10201 cggcaagcgc acctcgtccc gggctccgct ccccctcttc ctcgcgggcg gccaatccgt
    10261 cgctcttgcc cctgccacgt catccctcca agggccatgc ctcatcgccc ttttctctga
    10321 gctcccgggc gcggaggtct cctggaagcg agagctgctc gccttcctcg caagccgcct
    10381 cctcagcccg accgaagacg ccgatgaccc ctccccgcca gcaggcgctc gttcgctgcg
    10441 gctccccgag gcgctcgtcc tcggctcttc gccggcgttc gcctcctttc tcgcgacgct
    10501 cgaggcgacg gtcggcagcg gcctcgacgc gcttcttacc ggcgagacgg gcacgggcaa
    10561 ggagctgatc gcccgcgcga ttcacgactc gggtccccac cccgacggtc cattcgtcgc
    10621 catcaactgt gcggccatcc cgaacgagct cctcgaggcc gagctattcg gcgtcacgcg
    10681 cggggccgcg acgggcgtcg aagcgcgccc gggtcggttc ctccaggcga acgggggcac
    10741 gctctttctc gacgagatcg gcgagctgcc ggagcccctc caggccaaac tcctccgagt
    10801 cgtccaggaa cgcgaggttc tctcgctcgg gggcgcctcc gcacggagga tccacgtccg
    10861 gatcgttgcg gcaacgaacc gcgacctcct ccgacggatg aaggaaggcc tcttccgacc
    10921 cgacctctac taccgcctct ccgcgctcca gtaccacgtc ccgcccctgc gcgaccggcg
    10981 cgaagacatc cccgccctcg tcctcgcctt cgcctcccgc tccgcagcct ccctcggccg
    11041 ccgcgtccgc ggcgtcagcc gcccggccct cgacgtcctt ctatcgcacg actggcccgg
    11101 caacgtccgc gagctcgaga ccgccgtcca gcgcgccgtc ctcctctgcc cgtccggcgg
    11161 cacgctcctc gctgagcact tctcacaggt gaggtgggct gtcgaagaga gggcgaggga
    11221 ggcggttgtc ctcacgcccg tcgacgtgct cggcgctccc cagccgtgtt cccctcctcc
    11281 gttcgcggtc cccggcgacc tcgacctcga ggcccggcgc aacgccgtcg agaaggagac
    11341 gatcgagcgc gccctcgcga cggccaaggg gaacaagtcc ctcaccgcga ggctcctgag
    11401 gatctcgagg aacggcctgg cggcgaggat gaaagcgctc gggatcggag agtagccggc
    11461 gttcttcgcc cgcacctcaa accgcagcag ctcacgacgt cgcctgcgcg accattgagc
    11521 caatgctcgc agcgcgcacg aggccgtgcc cttaacgcga tcaggtccgg cctcacacat
    11581 gcgagcactg tcgccgggat ttcatgctcg cgcccgccga gaccgtttca gaccgatttc
    11641 cgcttccgac gcgcaccact cccggcactg cgggcaggcg tcttgctcac gaggtctcga
    11701 aggtcggcag gcaggccgta cgaacagaca ggaggccact cgaatgagga acgctatcag
    11761 caggcgaatc gccctcgttc tctctcttgc tcctctctca ttcccggttc aggcccaggt
    11821 ccggggcggg cgcgcccagg gggccatcgt cgtcagcgag agcgcgaatg tctaccgcaa
    11881 ctccgacggc gacgccgtcg aggcggtcgt tagccgcggc gacttcgtcg ggagcatcac
    11941 gggttcagag ctcctttccg cccagtacct tctcgacgag gtgaacggac gcgtccacgt
    12001 catgtacttc aagaacaaag agcagaaggg cacgatgttc accgcctgga tgaatcccgc
    12061 agacctggct tcgttcttct acgagtgcgg atgcggaccc gaagggaaag agtgtgcacc
    12121 ctaccagttg aagttcatgt cccgacggtg gaacgcctgt tacgtcgagg ccagggacaa
    12181 gaagctcgcc gagctgaagg agaaatgggc ctcgggcggc ggcggcgaga cgaattcgtc
    12241 ccctacggcg cccccagcga agactgcgtc ggcagaccgc cccctttcga atgccgacgt
    12301 catcaacatg acgacagccg gcctcgacga agctctgatc atctcgaaga tagaaaaggc
    12361 ctccacggag gccctcgact cctcgccgga cggcctcgta gcgctgaaac aggctggtgc
    12421 gacgaaagcc gtcatcgccg ccgtctcgcg tcgagtcgag atgcggcagt agttcacgcc
    12481 acttccggga cgaggcgcca agccgaaccg cgggcgcctc gcccacggca cgcaacgccg
    12541 cgtgacgacc tgtcaatcac ttgccgctcc ccgtaacact gcttcccgcg acgagactct
    12601 cctgttctga tccttgcttc gcctctcgac gtcctggccc aggcgccggc cgtcagtacg
    12661 ccggggcctt gctgcgctcc ttcgcgaggt ctgtactcct cactgcgaaa gatagggccg
    12721 gttaccagtg caatgcggct ttgacgtagg ccacgtcagg atggtcgaag aagcaacgga
    12781 cgagggagcg gtcgagggcg atcccagtca tggcgttctc gacggcggca tcgaagtcct
    12841 cggtggcgaa aacgggggtc cgtctgaaga gtcccttcag ctgggaccag acgtgctcgt
    12901 ccggattcgt ctccggtgcg taggccagaa ggaaatgcac cacgatccga tcctcgtgcg
    12961 cggcgagcca gcgtcgagtg gc
//
LOCUS       DAOONL010000092        12975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5297_length_12975_cov_19.193344, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000092 DAOONL010000000
VERSION     DAOONL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_12975_cov_19.193344"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLP50_12610"
     CDS             complement(<1..807)
                     /locus_tag="PLP50_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPA52431.1"
                     /translation="MTTAPEGSTVPTSSTPPGTGGLRAALERIAAPARVLDRPLDRIA
                     FASDASFYRLIPKAVVLAESVDEVAALFRLARETKVPVTLRAAGTSLSGQAVTDGLLV
                     EVARHFRTVKVEDGGRRVRTQPGVIGASVNAALRPYRAKMGPDPASINTCTMGGILSN
                     NSSGMCCGVTQNAYHTLESLTFLLPSGTRIDTAAPDADARFRAAEPTLWQGLLDLKAE
                     LEADPALVARIRSKYRMKNTTGYSLNAFLDFEKPVEIFRNLLVGAEGTLAF"
     gene            complement(884..1987)
                     /locus_tag="PLP50_12615"
     CDS             complement(884..1987)
                     /locus_tag="PLP50_12615"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPA52432.1"
                     /translation="MKLVPILARAAAAAALAAALLPAPAPAQAVIKVNENVNFKLGFQ
                     FQGWADWSQSASTGGYAQNLFARRARLLVGGQVAKDVTFFFQTDSGNLGKVNPAGAKQ
                     TNTGFVIQDAFVSWKLNDALTLEAGHFLVPLSRNVLQSTLSFFTLDISPTSTVMAGPT
                     QTNGLRDNGFQAKGYLVDGGRLEYRAAVMQGVRDSASRNSFRTAGYLQYNFFETERGY
                     VFAGTNLGKKKVVNVSAGFDVQKDYRAFSGDVFATLPVAKGDEVGGQVQWIRYDGGDF
                     LAAIPEQDDLLVEAAYYLSAAKVQPFLKYESQSFANAADEGKDVERLGGGLNWYISGQ
                     NLKLTAQYLRVEPKSSAVPSTDQFTLQLQAFYF"
     gene            complement(1996..3693)
                     /locus_tag="PLP50_12620"
     CDS             complement(1996..3693)
                     /locus_tag="PLP50_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HPA52433.1"
                     /translation="MTWTQIYTPVAGNLLLSALVAALPVVVLLGLLAFFHVKAHKAAL
                     LGLATSLGVAIFVYGMPTKLALTSAANGALYGLFPIGWIVLCAIFVYDLTVKSGDFEV
                     VKHTVAGLADDRRIQALLIAFSFGAFIEGAAGFGTPVAISAAMLIGLGFRPLLAAGIA
                     LIGNTAPVAYGALGTPIVVLASVTGLDVQALSAMAGRQLPFFSLIVPFWLVWAMSGRK
                     AMWEVWPACLTSGLSFAITQFLVSNYHGPWVVDIAGAIVSMGATVLLLKFWQPKKTWT
                     FDHENHADRAAEAAARPVHTPKRALMAWMPWILLSVFVFAWGLPQVKDVLNGGKKDSP
                     NVLKGVSMLKWEVPGLHKAVIKAPPVVKEEKPEEAVFTFNWLSATGTSLLLSGILSGL
                     LLGFSLKEIGSVFWGTLMRVRISLLTIGAMLALGFSTRYAGLDATMGLAFASTGLLFP
                     FFSPLLGWLGVALTGSDTSSNVLFGGLQMISAEKLGINPVLAAAANSSGGVMGKMIDA
                     QSIVVASVATNQQGQEGTILRYVFWHSIVLACLVGVLVFLQAYVFPWMIPTVSAVAK"
     gene            complement(3849..4496)
                     /locus_tag="PLP50_12625"
     CDS             complement(3849..4496)
                     /locus_tag="PLP50_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA52434.1"
                     /translation="MSTTILLADDHRIVREGLRTLLSQQPEMEVVGEAADGREAVSQA
                     LLLRPAVVVMDIAMPELNGVEATRLILAELPATRIVALSMYADRRFVAEVLRAGALGY
                     VLKDGAFEELALAIRTTLAGKTYLSPSIAGLVVEELVRKAAAPGSPSLASLTPRERQV
                     LKLLAGGKRPREIAQELAISSKTVEVHKQNLMNKLEIHSAAELTRFAIREGLATP"
     gene            complement(4493..6814)
                     /locus_tag="PLP50_12630"
     CDS             complement(4493..6814)
                     /locus_tag="PLP50_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA52435.1"
                     /translation="MRLRALLAVLALLLGPTAGAAGAATSGADAASGHRVLVLHSYYK
                     GYKWADDEHRGITSVLEPVLGVENVFVEYMDTKRFYEPRHVAQLPEVYRRKYADHRID
                     LVVGVDDRAVDFLVEHRDEVFPGTPVVFCGVNYFRPEKLKGRGLFTGVSEDADLGASL
                     ETALDLHPGTRRIYVVNDDTETGLAVRARLAELGPAVRGVPMEPLDGLSMERLLARLR
                     ALPPDSLVFFAFFFRDADGRVFGYDESARLVTGASNAPVYGAWDFNLGLGIVGGRLTS
                     GFDQGEAAARIALRVLRGEAPESIPVLRGLPGRYLFDHLVLRRWGIPASRLPEGSTVL
                     NLPESAFARYRKVFLAGAGALVLLGVLNAILALNISRRKLAEEALRRHQETLEETIRL
                     RSEELSRANEELRRDILERERTERALRVAEANLRSIFENAAEGIYQSTPSGRFLTVNP
                     ALAAMAGYASPAEMTEAISDIRRDFYVDPSSRERFEEALVEEEEVRGFESLVRRRDGA
                     LIWVLENARAVRDEKGRLDHYEGIVQDISERRQMAEKLDRSRRKLRGLAAEITRVEER
                     ERRAIAAQLHDQLGATLSMGKVKLGSLRQEAQGTPLAAGIEAVHTLVGEAVRETRELT
                     WELSPPVLYQLGLEAALEWLGESFEERHGIAFRFEREGERRDLGDERRFLVFAAVREL
                     LLNVVKHADADGASVRLRWLGGGVEVAVSDDGKGFEAAAPAPDESRSFGLFSIQERFD
                     DLGGSVAIRSRPGEGTTVALVLPYRAEAGEESE"
     gene            6964..7527
                     /locus_tag="PLP50_12635"
     CDS             6964..7527
                     /locus_tag="PLP50_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPA52436.1"
                     /translation="MFEAFRARAEAVSAEVHRVGGSTEALAFVAELLAKEGVADQAGA
                     RAVWARGPLVAGIDREAFASRVPGLTFDVTREAAAGSRVGVSEMEWGLADTGTVVQDA
                     TDPALRLASTLPEIHVALLPAVRILPGLADALPRLDPRSAPYIAFVTGPSRTADIERV
                     LTIGAHGPSRLVVVVHDGEAARAEVTP"
     gene            7530..9731
                     /locus_tag="PLP50_12640"
     CDS             7530..9731
                     /locus_tag="PLP50_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPA52437.1"
                     /translation="MTFRKEIAGALGDPNLASALGRFYEAYPVSRAKAYQGIDFEAVR
                     TRIAEVKGDATGRLPELAERFAKEAGARGAKVVLLKSPEEVRRYIVDLAREKGVRKVA
                     KSKSMASEEVHLNRALTEAGIDVKETDLGEWIIQLAGQRPSHMVMPAIHLSKEDVAEI
                     FSNEVGERLSTDIPRLVKVARKELRSAFLEADMGISGANIAVAETGTLVLVTNEGNAR
                     LVTSLPRIHVAIVGLEKLVERFEDVVPILTALPRSATGQLLTSYVSMITGAAPNTDGT
                     PKELHVVLMDHRRTEMAADPVFKEALQCIRCASCLNVCPVYRHVGGHVFGDVYTGGIG
                     TILTAWTGAMERSKEIQGLCIQCGNCVGVCPGKIDIPELIVEVRRRQVQEKGQPFVQK
                     AIFSVVNNRRLFHSVLRSASLAQKPFEKDGFVRHLPLFLSGLADFRSLPAIAERPFRD
                     AFRAIEQKVFRATEPAEKGAEAPVAPRTRKAAFYAGCLIDFAYPGMGEDVVKVLNAGG
                     VEVTFPEAQTCCGAPARYSGAYEVAARNAIDNIEALLAEEVDWVVSACPTCTTALRHE
                     FARSLRSTGKTEWVARAEALAAKVIDFSSLVTRLVDEGSLKLDPRKVPAVTYHDSCHL
                     KRTLHVSEEPRALLRRAGHEVVEMKESDTCCGMGGSYSLKFPEISKPILQKKLANIRD
                     TKASAAVMDCPGCVMQIRGGLDKSNDTVEVQHIARLLARAVAEHAGDGSEA"
     gene            complement(9744..11753)
                     /locus_tag="PLP50_12645"
     CDS             complement(9744..11753)
                     /locus_tag="PLP50_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPA52438.1"
                     /translation="MSRVRRVLLLALGALLAGAAARGFLAVVEAALEGRIRARIVSEA
                     AERGFRADVDGVTVRLPFRARLSGLVIAGHGVRARLERVDVRVSLLGRGAVGRLRRVE
                     VGALAADLPEGLSVRVAPSAWDVSVEAGLLALRGRNGEGGIAFERPGGGAFRLRLDRL
                     ALTRLVLLRRDGAFVGDLGEASGILDLTPAADGSLAVETNLISRGVRLSMPPDEEVTE
                     AVPLGEPFDAEGTLTATLFREAAFLEVACLSGDAAGLRGTARGVAAFAPRDAWLDVGL
                     EVPRLELGPLLAASGLELPAEDGDLGSAELVARIEGRMLDPKSWVVEERLDFRPPARP
                     LPEVARLRGPFRRVMTGRDGRRTPVTVDSESPDFVPLEAVPAHFVRGLLTSEDADFWG
                     HPGLDLGEVPVAASMNWVRGRRARGASTITQQLAKNLFLTRERSYRRKLQEAALALHL
                     ESTLGKRRILEIYLNVIEWGPGLHGLGPAARHYFGKNPQDLTPREGAFLVALIPGPVK
                     YQSSFAGGVLSRGFTRLVNGVLRRLASVGMLTDEELKAELETPLRLRWTPADVAGEEL
                     VAGGAGSDALEPDEEVEEEVGVPILAPVEPAPATDESASSGLPASSSLPPAAAGPVPA
                     AGTAPGPAATPTPAARPPARESPGPVLPPPAPTPRPTPRATAPAG"
     gene            11887..12516
                     /locus_tag="PLP50_12650"
     CDS             11887..12516
                     /locus_tag="PLP50_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA52439.1"
                     /translation="MDQEEKSERSRAQILEAALDLFSRQGYRGTSIRDISKAAGVSTG
                     SVYHHFHDKEELFDTLLLQYWTAIESPDFPFNRALASGAFPDRLEELAEAAEESVQSW
                     ERHVALIYVDVVEMQGTHIQRFYAGMADRFEAFLRRYRAQDDFETRLAPGVSPLSAVM
                     LASRIFLQYYAVERLFRVPDQFGKPSREAIRDISEILRRGMLHERRGAR"
     gene            12594..>12975
                     /locus_tag="PLP50_12655"
     CDS             12594..>12975
                     /locus_tag="PLP50_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPA52440.1"
                     /translation="MSLVGDRLDAVRRVPLAALPTPVQDAPRLAAHLGLARLLVKRDD
                     LTGLAMGGNKARKLEYDLAPATTGEYDTVLTVGGTQSNHAVLTAAAARRLGLDAKLVL
                     GGPDVSRRKGNLLLGALYGAETRYL"
BASE COUNT         1985 a         4595 c         4604 g         1791 t
ORIGIN      
        1 gaacgcgagc gtcccctccg ccccgacgag gaggttccgg aagatctcga cgggcttctc
       61 gaagtcgagg aaggcgttca gcgagtaacc ggtggtgttc ttcatccggt acttcgaccg
      121 gatgcgggcg acgagcgccg ggtccgcctc gagctcggcc ttcaggtcga ggagcccctg
      181 ccagagggtc ggctccgccg cgcggaagcg cgcgtcggcg tcgggggccg ccgtgtcgat
      241 ccgcgtcccc gagggaagga ggaacgtgag cgactcgagc gtgtggtagg cgttctgggt
      301 cacgccgcag cacatgccgc tcgagttgtt cgagaggatc ccgcccatcg tgcaggtgtt
      361 gatggaggcg gggtcgggcc ccatcttggc gcggtagggg cggagggccg cgttcaccga
      421 ggcgccgatg acgcccggct gcgtccgtac gcgcctcccg ccgtcttcga ccttcaccgt
      481 ccggaagtgg cgcgcgacct cgacgaggag cccgtccgtg acggcctgcc ccgagagcga
      541 ggtccccgcg gcgcgaagcg tcaccgggac cttcgtctcg cgggcgagcc ggaagagtgc
      601 ggcgacctcg tcgaccgact cggccaggac gaccgctttc gggatcagcc tgtagaagct
      661 cgcgtccgag gcgaaggcga tccggtcgag gggccggtcg aggacgcgcg cgggcgcggc
      721 gatccgctcg agggcggcgc ggaggccgcc cgtaccgggc ggcgtgctcg acgtcgggac
      781 cgtggaaccc tccggggcgg tcgtcatcag aggccctcct ggacgcgcag agagggggcg
      841 gcgccggggc gattctcccc cgccggcgcc gccccgcccg ggatcagaag tagaacgcct
      901 ggagctggag cgtgaactgg tcggtggagg ggacggccga gctcttcggc tcgacccgca
      961 ggtactgggc cgtgagcttg aggttctgcc ccgagatgta ccagttcagc ccgcctccga
     1021 gacgctcgac gtccttccct tcgtccgccg cgttcgcgaa cgactggctc tcgtacttga
     1081 ggaacggctg caccttcgcg gcggagaggt agtacgccgc ctcgacgagg aggtcgtcct
     1141 gctcggggat cgccgcgagg aagtcaccgc cgtcgtatcg gatccactgg acctggccgc
     1201 ccacctcgtc ccctttcgcg acggggagcg tcgcgaagac gtcgccgctg aaggcccggt
     1261 agtccttctg gacgtcgaag ccggcgctca cgttgacgac cttcttcttc ccgaggttcg
     1321 tgccggcgaa gacgtagccc cgctccgtct cgaagaagtt gtactggagg tagcccgccg
     1381 tccggaacga gttgcgggag gcggagtcgc gcacgccctg catcacggcc gcgcggtact
     1441 cgagccgccc gccgtcgacg aggtacccct tcgcctggaa gccgttgtcg cgcaggccgt
     1501 tcgtctgcgt cggtccggcc atgaccgtcg aggtcgggct gatgtcgagc gtgaagaacg
     1561 agagggtcga ctggagaacg ttccggctga gcgggacgag gaagtgcccg gcctcgagcg
     1621 tgagcgcgtc gttcagcttc caggagacga acgcgtcctg gatgacgaag cccgtgttcg
     1681 tctgcttcgc gccggccggg ttcaccttgc cgaggttccc gctgtcggtc tggaagaaga
     1741 acgtcacgtc cttcgcgacc tgccccccga cgaggaggcg ggcgcggcgg gcgaagaggt
     1801 tctgggcgta gccgcccgtc gaggcgctct ggctccagtc ggcccacccc tggaactgga
     1861 agccgagctt gaagttcacg ttctcgttca ccttgatgac ggcctgtgcg ggggcgggcg
     1921 cggggagaag ggcggcggcg agcgcggcgg cggccgcggc gcgggcgagg atcggaacga
     1981 gcttcatctt tctcctcact tcgcgaccgc cgagacggtc gggatcatcc aggggaagac
     2041 gtaggcctgc aggaagacga ggacgccgac gaggcaggcg aggacgatcg agtgccagaa
     2101 gacgtagcgg aggatcgttc cctcctggcc ctgctggttc gtcgcgacgc tcgccacgac
     2161 gatgctctgc gcgtcgatca tctttcccat gacgccgccg ctgctgttcg ccgcggccgc
     2221 gaggaccggg ttgatgccga gcttctcggc gctgatcatc tgcagcccgc cgaagaggac
     2281 gttcgaggag gtgtcgctcc ccgtgagcgc gacgccgagc cacccgagga gcggcgagaa
     2341 gaacgggaag aggaggccgg tcgaggcgaa cgcgagcccc atcgtcgcgt cgaggcccgc
     2401 gtagcgcgtc gagaacccga gcgcgagcat cgccccgatc gtcaggagcg agatccgcac
     2461 ccgcatgagc gtcccccaga agaccgatcc gatctccttc agagagaagc cgaggaggag
     2521 gccgctcagg attcccgaga ggaggaggct cgtcccggtg gcggagagcc agttgaacgt
     2581 gaagaccgcc tcctcgggct tctcctcctt cacgaccggc ggcgccttga tgaccgcctt
     2641 gtggaggccg gggacctccc acttcagcat cgagacgccc ttcaggacgt tcggggagtc
     2701 cttcttcccg ccgttcagga cgtccttcac ctgggggagc ccccacgcga agacgaagac
     2761 cgagaggagg atccagggca tccaggccat gagagcccgc ttcggcgtgt ggaccgggcg
     2821 ggcggctgcc tcggcggccc ggtcggcgtg gttctcgtgg tcgaaagtcc aggtcttctt
     2881 cggctgccag aacttcagga ggaggacggt cgcccccatc gagacgatcg cgcccgcgat
     2941 gtcgacgacc caggggccgt ggtagttcga gacgaggaac tgcgtgatcg cgaagctgag
     3001 gccggaggtg aggcaggcgg gccagacctc ccacatcgcc ttgcggcccg acatcgccca
     3061 gacgagccag aacgggacga tgagcgagaa gaaggggagc tgccggccgg ccatggccga
     3121 gagggcctgg acgtcgagcc cggtgacgga ggcgaggacg acgatcggcg tcccgagcgc
     3181 gccgtaggcc acgggcgccg tgttgccgat gagcgcgatg ccggccgcga ggagcggccg
     3241 gaagccgagg ccgatgagca tcgcggccga gatggcgacc ggcgtgccga agcccgcggc
     3301 cccctcgatg aacgccccga acgagaaggc gatcaggagg gcctggatcc ggcggtcgtc
     3361 cgcgaggccc gcgacggtgt gcttgacgac ctcgaagtcg ccggacttca ccgtcaggtc
     3421 gtagacgaag atcgcgcaga ggacgatcca gccgatcggg aagaggccgt agagcgcgcc
     3481 gttcgcggcg gacgtcaggg cgagcttcgt cggcatcccg tagacgaaga tcgcgacccc
     3541 gagcgaggtc gcgaggccca ggagcgccgc cttgtgcgcc ttcacgtgga agaacgcgag
     3601 aagcccgagc agcacgacga ccggcagcgc tgccacgagg gccgacagca ggaggtttcc
     3661 tgccaccggc gtgtagatct gggtccaggt caacatcagc ccctcctcat cccgaccaga
     3721 tggacctaag gatgggagtg acgatactta ggtgcaatga tgaggacaca tcaatttatt
     3781 ggtgtttata cctagaaatt tcgactagaa cgggagcgtg gccctgctcc ccccccgggg
     3841 cagcccggtc agggtgtcgc gagcccctcc cgaatggcga agcgcgtcag ctcggcggcc
     3901 gagtggatct cgagcttgtt catgaggttc tgcttgtgga cctcgaccgt cttgctgctg
     3961 atcgcgagct cctgggcgat ctcgcggggg cgcttcccgc cggcgaggag cttcaggacc
     4021 tgccgctctc tcggggtgag agaggcgagc gacggagagc cgggcgcggc tgccttccgg
     4081 acgagctcct cgacgacgag cccggcgatc gaggggctca ggtaggtctt ccccgcgagc
     4141 gtcgtccgga tcgcgagggc gagctcctcg aacgcgccgt ccttcaggac gtagccgagc
     4201 gcccccgcgc ggaggacctc cgcgacgaag cgccggtcgg cgtacatcga gagcgcgacg
     4261 atgcgcgtcg ccgggagctc cgcgaggatg agccgcgtcg cctcgactcc gttcagctcc
     4321 ggcatcgcga tgtccatgac gacgacggcg ggccggagga ggagcgcctg cgagaccgcc
     4381 tcgcgaccgt ccgccgcctc cccgacgacc tccatctccg gctgctgcga aaggagggtt
     4441 cggaggccct cgcggacgat ccggtggtcg tccgcgagca ggatcgtcgt gctcattcgc
     4501 tctcctcccc cgcctccgcc cggtaaggta gcacgagggc gaccgtcgtc ccctcgcccg
     4561 gacgggagcg gatcgcgacg cttccgccga ggtcgtcgaa ccgctcctgg atgctgaaga
     4621 gcccgaagct ccggctctcg tccggcgcgg gcgcggccgc ctcgaagccc ttcccgtcgt
     4681 cgctcaccgc gacctcgacg ccgccgccga gccagcgaag ccggacggag gccccatcgg
     4741 cgtccgcgtg cttgacgacg ttcaggagga gctcgcggac cgcggcgaag acgaggaacc
     4801 ggcgttcgtc gccgaggtcc ctcctctcgc cctccctctc gaaccggaac gcgatcccgt
     4861 gcctctcctc gaagctctcc ccgagccact ccagggccgc ctccagaccg agctggtaga
     4921 ggacgggcgg gctcagctcc cacgtcagct cccgggtctc ccggaccgcc tcgccgacga
     4981 gcgtgtgcac cgcctcgatc ccggcggcga gcggcgtccc ctgcgcctcc tgccggaggg
     5041 agccgagctt caccttcccc atcgagagcg tcgccccgag ctggtcgtgg agctgcgcgg
     5101 cgatcgcccg ccgctcccgc tcctcgaccc gcgtgatctc cgcggcgagc ccgcgcagct
     5161 tccggcgcga ccggtcgagc ttctccgcca tctgccgacg ctcggagatg tcctggacga
     5221 tcccctcgta gtggtcgagc cgccccttct cgtcccggac ggcccgcgcg ttctcgagga
     5281 cccagatgag cgcgccgtcc ctcctcctca cgagcgactc gaagccgcgg acctcctcct
     5341 cctcgacgag ggcctcctcg aagcgctccc ggctcgacgg gtcgacgtag aagtcgcgcc
     5401 ggatgtccga gatcgcctcc gtcatctcgg ccggagaggc gtagccggcc atcgccgcga
     5461 gggcggggtt caccgtcagg aaccgcccgg acggggtcga ctggtagatc ccctcggccg
     5521 cgttctcgaa gatgctccgg aggttcgcct cggcgacccg gagagcgcgc tcggttcgct
     5581 cgcgctccag gatgtccctc cgcagctcct cgttcgcccg cgagagctcc tccgatcgca
     5641 gccggatcgt ctcctcgagc gtctcctggt gccggcggag cgcctcctcg gcgagcttcc
     5701 gccggctgat gttcagcgcg aggatcgcat tcaggacccc gaggaggacg agggccccgg
     5761 cgccggccag aaagaccttc cggtagcgtg cgaaggccga ctccgggagg ttgaggaccg
     5821 tgctcccctc cgggagccgc gaagcgggga tcccccagcg cctgagcacg aggtggtcga
     5881 agaggtaccg gcccggcagg ccgcgaagga ccgggatcga ctccggcgcc tcgccgcgca
     5941 ggacccggag cgcgatccgt gcggcggcct ccccctggtc gaacccgctc gtgagcctcc
     6001 cgcccacgat cccgaggccg agattgaagt cccaggctcc gtagaccggg gcgttcgagg
     6061 cacccgtcac gaggcgcgcg ctctcgtcgt agccgaagac gcggccgtcg gcgtcgcgga
     6121 agaagaacgc gaagaagacg aggctgtcgg gtgggagcgc ccggagtcgc gcgaggagcc
     6181 gctccatcga gaggccgtcg agcggctcca tcgggacgcc ccggacggcg ggtcccagct
     6241 cggcgagccg ggccctcacg gcgaggccgg tctccgtgtc gtcgttgacg acgtagatgc
     6301 gccgcgtgcc ggggtggagg tcgagcgcgg tctcgaggct cgccccgagg tccgcgtcct
     6361 cgctcacgcc ggtgaagagg ccccgcccct tcagcttctc ggggcggaag tagttgacgc
     6421 cgcagaagac gacgggcgtc ccggggaaga cctcgtcgcg gtgctcgacg aggaagtcga
     6481 ccgcgcggtc gtccacgccg acgacgaggt cgatccggtg gtccgcgtac ttccgcctgt
     6541 agacctccgg gagctgggcg acgtgcctcg gctcgtagaa gcgcttcgtg tccatgtact
     6601 cgacgaagac gttctcgacg ccgaggaccg gctcgaggac cgaggtgatt ccgcgatgct
     6661 cgtcgtcggc ccacttgtag cccttgtagt acgagtggag gacgaggaca cggtggccgg
     6721 acgccgcgtc ggccccggac gtggcagctc ccgcggcgcc ggcggtcggc ccgagaagga
     6781 gcgcgagcac ggcgaggaga gcgcgaagcc tcattccgac cccgggaggg aggctaacag
     6841 gccgccccgg acgctccagt cgaccgaagc tcaccggcca agaataccct agatattttt
     6901 acgtagattc agataagctg atccgtggtc gccggagagc gctccgggcc cgggaggcga
     6961 gcgatgttcg aggcatttcg cgcacgagcc gaagccgtca gcgccgaggt ccaccgcgtc
     7021 ggcgggagca ccgaggcgct cgcattcgtc gcggagctcc tcgcgaagga aggggtcgcg
     7081 gaccaggccg gcgcgcgggc ggtctgggca agaggccccc tcgtcgcggg gatcgaccgc
     7141 gaggcgttcg cttcccgcgt ccccgggctg acgttcgacg tcacgcggga ggccgcggcc
     7201 gggagccgcg tcggcgtcag cgagatggag tgggggctcg ccgacacggg caccgtcgtc
     7261 caggacgcga ccgaccccgc gctgcggctg gcctcgacgc tgccggagat ccacgtggcg
     7321 ctcctcccgg cggtccggat cctcccgggc ctcgcagacg cccttccgcg cctcgatccc
     7381 cgatccgccc catacatcgc gttcgtgacc gggccgagcc ggacggccga catcgagcgc
     7441 gtcctgacga tcggggccca cggtccgtcg cgcctcgtcg tcgtcgtcca cgacggcgag
     7501 gccgcgcggg cggaggtgac gccgtgacga tgacgttccg caaggagatc gccggggcgc
     7561 tcggcgaccc gaacctcgcc tccgccctcg gccgcttcta cgaggcctac cccgtcagcc
     7621 gcgcgaaggc ctaccaaggg atcgacttcg aggccgtccg cacgaggatc gccgaagtga
     7681 agggagacgc gacgggccgg ctccccgagc tcgccgaacg cttcgcgaag gaggccggcg
     7741 cgcgcggcgc gaaggtcgtc ctcctgaaga gccccgagga ggtccggcgg tacatcgtcg
     7801 acctcgcccg agagaagggc gtgaggaagg tcgcgaagtc gaagtcgatg gcctccgagg
     7861 aggtccacct caacagggcg ttgaccgaag ccgggatcga cgtgaaggag accgacctcg
     7921 gcgagtggat catccagctc gccggccagc gcccctcgca catggtgatg cccgcgatcc
     7981 acctgtcgaa ggaggacgtg gcggagatct tcagcaatga ggtcggcgaa cggctttcga
     8041 ccgacatccc ccggctcgtg aaggtggcgc gcaaggagct ccggagcgcc tttctcgagg
     8101 ccgacatggg gatctccggc gccaacatcg cggtcgccga gaccgggacg ctcgtcctcg
     8161 tgacgaacga ggggaacgcg cgcctcgtca cgtcgctccc gcggatccac gtcgccatcg
     8221 tcggcctcga gaagctcgtc gagcggttcg aggacgtcgt cccgatcctg accgccctgc
     8281 cgcggagcgc gacggggcag ctgctgacga gctacgtctc gatgatcacg ggcgccgcgc
     8341 cgaacaccga cgggacgccg aaggagctcc acgtcgtcct gatggaccac cggcggaccg
     8401 agatggcggc ggacccggtc ttcaaggagg cgctccagtg catccgctgc gcctcctgcc
     8461 tgaacgtctg ccccgtctac cggcacgtcg gcgggcacgt cttcggcgac gtctacacgg
     8521 gcggcatcgg gacgatcctg accgcctgga ccggcgcgat ggagcgctcg aaggagatcc
     8581 aggggctctg catccagtgc ggcaactgcg tcggcgtctg ccccgggaag atcgacatcc
     8641 ccgagctgat cgtcgaggtc cgccgccggc aggtgcagga gaaggggcag cccttcgtcc
     8701 agaaggcgat cttctcggtc gtcaacaacc gccgcctctt ccactcggtc ctccggtccg
     8761 cttcgctcgc ccagaagccg ttcgagaagg acggcttcgt gaggcacctg ccgctcttcc
     8821 tctccggcct cgccgatttc cggagcctcc cggcgatcgc cgagaggccg ttccgcgacg
     8881 cgttccgagc gatcgagcag aaggtcttcc gtgcgacgga gcccgcggag aagggcgcgg
     8941 aggcgccggt cgcgccacgg acgcggaagg ccgccttcta cgccggttgc ctcatcgact
     9001 tcgcctaccc gggaatgggc gaggacgtcg tgaaggtcct gaacgcggga ggcgtcgagg
     9061 tgacgttccc cgaggcacag acctgctgcg gcgccccggc gcgctacagc ggcgcgtacg
     9121 aggtcgccgc gcggaacgcg atcgacaaca tcgaggcgct cctcgcggag gaggtcgact
     9181 gggtcgtctc ggcctgcccc acctgcacga cggcgctccg ccacgagttc gcgcggtcgc
     9241 tccggagcac ggggaagacc gagtgggtcg cgagggccga ggcgctcgcg gcgaaggtga
     9301 tcgacttctc ctcgctcgtg acgcggctcg tcgacgaggg ctcgctgaag ctcgacccga
     9361 ggaaggtccc cgcggtgacg taccacgact cctgccacct gaagcggacg ctccacgtct
     9421 ccgaggagcc gcgtgcgctc ctccggaggg ccggccacga ggtcgtcgag atgaaggagt
     9481 cggacacctg ctgcggcatg gggggctcct actccctgaa gttccccgag atcagcaagc
     9541 ccatcctcca gaagaagctc gcgaacatcc gcgacacgaa ggcgtccgcc gccgtcatgg
     9601 actgcccggg ctgcgtcatg cagatccgcg gcggtctcga caagtcgaac gacacggtcg
     9661 aggtgcagca catcgcgcgg ctcctcgcgc gggccgtcgc cgagcacgcg ggagacggct
     9721 ccgaggcctg acggaaggtc gcttcagccc gccggtgccg tcgcgcgcgg cgtcggtcgc
     9781 ggcgtgggcg ccggtggtgg aagcaccggg ccggggctct ccctcgcggg cggcctcgcg
     9841 gccggcgtcg gcgtcgcggc gggacccggt gccgtccccg cagccggcac ggggccggcc
     9901 gcggccggcg gcaaggagga agacgcggga aggccggacg aggccgactc gtcggtcgcg
     9961 ggcgccggct cgacgggagc gaggatcggg acaccgacct cttcctcgac ctcctcgtcc
    10021 ggctcgagcg cgtccgatcc agctcccccc gccacgagct cctcgcccgc cacgtcggcc
    10081 ggcgtccacc gcagccgcag cggcgtctcg agctcggcct tcagctcctc gtcggtcagc
    10141 atcccgaccg aggcgaggcg gcggaggacg ccgttcacga gccgggtgaa gccccgcgac
    10201 aggaccccgc ccgcgaacga cgactggtac ttgaccgggc ccgggatgag cgcgacgagg
    10261 aaagcccctt cgcgtggcgt caggtcctgc gggttcttcc cgaagtagtg gcgcgcggcc
    10321 ggcccgaggc cgtggaggcc cggcccccac tcgatgacgt tcaggtagat ctcgaggatc
    10381 cgccgcttcc cgagcgtcga ctccaggtgc agggccagcg ccgcctcctg gagcttccgg
    10441 cggtagctcc tctcgcgcgt caggaagagg ttcttcgcga gctgctgcgt gatcgtcgaa
    10501 gcgccgcgcg cccttcggcc gcggacccag ttcatcgaag cggcgaccgg gacctcgccg
    10561 aggtcgaggc caggatgccc ccagaagtcg gcgtcttccg aagtcaggag gccacggacg
    10621 aagtgggcgg ggaccgcctc gagcgggacg aagtcggggc tctcggaatc gaccgtgacc
    10681 ggcgttcgcc gtccgtccct tccggtcatg acccgccgga acggccctcg cagcctcgcg
    10741 acctcgggga gcggccgtgc cggcggccgg aagtcgagcc gctcctcgac gacccacgac
    10801 ttcgggtcga gcatgcggcc ctcgatgcgg gcgacgagct cggcgcttcc gaggtcgccg
    10861 tcctccgcgg gaagctcgag gcccgaggcc gcgaggagcg gcccgagctc cagacgcggg
    10921 acctcgagcc cgacgtccag ccaggcgtcc cgcggagcga acgcggcgac gccccgcgcc
    10981 gtgcctcgaa gtcccgcggc gtctcccgac aggcaggcca cctcgaggaa ggccgcctcg
    11041 cggaagagcg tcgccgtcag cgtgccctcg gcgtcgaacg gctcgccgag cggcacggcc
    11101 tccgtcacct cctcgtcggg cggcatcgag agacggaccc ctctcgagat caggttggtc
    11161 tcgaccgcga ggctcccgtc tgccgcgggc gtcaagtcga ggattcccga cgcctcgccg
    11221 aggtccccga cgaacgcgcc gtcccgccgg aggaggacga gccgggtcag ggcgagccgg
    11281 tcgagccgga gccgaaacgc gccgccgccc ggccgctcga acgcgattcc cccctcgccg
    11341 ttacgccctc tcagcgcgag aagtccggcc tcgacggaga cgtcccacgc gctcggcgcc
    11401 acccgaaccg agagcccctc cgggaggtcg gcggcgagcg cgccgacctc gacgcgtcgc
    11461 agacgaccga ccgcgccgcg cccgaggagg ctgacccgca cgtcgacccg ctcgagccgg
    11521 gccctcacgc cgtgccccgc gatcacgagc cccgagaggc gcgcgcggaa cggaagccgg
    11581 accgtcacgc cgtccacgtc ggcccggaag ccgcgctccg ccgcctcgga gacgatcctc
    11641 gcgcggatcc tcccctcgag cgccgcctcg acgacggcga ggaagccccg cgccgccgct
    11701 cccgcgagga gagcgccgag cgcgagaagg aggacgcggc ggacgcggga cattcgaagc
    11761 cccgacttta ccgggtgacg gctccggaag gcacgggcat cgcaggccgc cgcggtgtcc
    11821 gggcgaaggc acccttgacg gcaggccgcc cgccccctac ggtagccggt cgaagcgaga
    11881 cgagggatgg accaggaaga gaaatccgag cgaagccggg cgcagatcct ggaggcggcg
    11941 ctcgacctct tctcgcgcca gggctaccgc gggacgagca tccgcgacat ctcgaaggcg
    12001 gccggcgtct ccacggggag cgtctaccac cacttccacg acaaggaaga gctgttcgac
    12061 acgctcctcc tgcagtactg gaccgcgatc gagagccccg atttcccgtt caaccgggcg
    12121 ctcgcttcgg gggccttccc ggaccgcctc gaggagctcg ccgaggccgc cgaagagagc
    12181 gtgcagagct gggagcgcca cgtcgcgctc atctacgtcg acgtcgtgga gatgcagggg
    12241 acgcacatcc agcgctttta cgcgggcatg gcggaccggt tcgaggcgtt cctccggcgc
    12301 taccgcgccc aggacgactt cgagacccgc ctcgctcccg gcgtctcgcc cctctccgcc
    12361 gtcatgctcg cgagccggat cttcctccag tactacgccg tcgagaggct cttccgcgtc
    12421 ccagaccagt tcggcaagcc ttcgcgggaa gcgatccggg acatctcgga gatccttcgc
    12481 cggggaatgc tccacgagcg ccgcggcgcc cggtaggagc tccgcgccgg ccgcgcggcc
    12541 atcgccgata ctcctcgttc gggcggccgg agggcctccc gggagggtcc ggaatgagcc
    12601 tcgtgggaga tcgtctcgat gccgtccgcc gggtgccgct cgcggcgctt ccgacccccg
    12661 tgcaggacgc cccgcgcctc gcggcgcacc tcggcctcgc acgcctcctc gtcaagcgcg
    12721 acgacctgac cggactcgcg atgggcggga acaaggcccg caagctggaa tacgacctcg
    12781 cgcccgcgac gaccggggaa tacgacaccg tcctcaccgt cggcggcacc cagtcgaacc
    12841 acgccgtcct gacggccgcc gctgcgcggc gcctcggcct cgacgcgaag ctcgtcctcg
    12901 gcggccccga cgtgtcccgg cggaagggga acctcctcct cggcgcgctc tacggcgccg
    12961 agacgcgcta cctcg
//
LOCUS       DAOONL010000093        12581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5540_length_12581_cov_13.813189, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000093 DAOONL010000000
VERSION     DAOONL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5540_length_12581_cov_13.813189"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..453
                     /locus_tag="PLP50_12660"
     CDS             25..453
                     /locus_tag="PLP50_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52441.1"
                     /translation="MADPKKPDRKVPSNPYVTGPTRILDASSSGPSARGGGEAPRPGV
                     RPELVTDGMKAHRREIEKRKRQASIEKMHLEMQTADERKAIWKWTFRVVLVLVILFAY
                     WKVQDAYGNQWPMWETWIVLAIVLFAGIGWLLWYLNKTEL"
     gene            complement(559..855)
                     /locus_tag="PLP50_12665"
     CDS             complement(559..855)
                     /locus_tag="PLP50_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52442.1"
                     /translation="MGDVFVIQCPDCDSTLKVDRETGAVLSHEPAAAKRKLGSIEEAA
                     AEIARRKERAEDLFAANVEREKHKSEILEKTFREALQKAQKEPPGRPRSPFDAD"
     gene            876..1322
                     /locus_tag="PLP50_12670"
     CDS             876..1322
                     /locus_tag="PLP50_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPA52443.1"
                     /translation="MPSGWKERLSELGISRRRRPAAARPGPSRPATTPKGRAGEDEAA
                     RFLEAQGLELLARNVRAGEGELDLVAAEGRCVVFVEVKWRRDASRGAPAEAVTPVKRR
                     RLLSAARAFLAEHPSRARRDVRFDVVAIEGEAGKIDWIRGAFDATD"
     gene            1309..4689
                     /locus_tag="PLP50_12675"
     CDS             1309..4689
                     /locus_tag="PLP50_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52444.1"
                     /translation="MRRTERGPRMRSWRAAALVAAATVFGLAPSAAEAGLRLESDGGS
                     GADVRAFASGGGAFWAGTRSGVLRGTSLDAAWRFDGLSGKPVSSVASLGDEVWAATGE
                     ELWRRSANGSWSLEALPSSVAFPTTLAVDASGTLWVGGLGVWRRSGASWVAAPSPGVG
                     IVTAMLADPAGLVVGLSAGSAARWNGSGWAPLNGGVGPGEAIRALASFGGTLWAGTNV
                     TLYWWGVGAWVADGPFGGHDVRALTTWGGALRAATADAGVLVRSGSAWTAASTGILPR
                     GVQAFLVAGTELLAGTSGGPVYRFWNGTWHPSAVTPVGAGVAADAFAYPAEAAGSQAG
                     AAVGVTRGGGADYLLRPCCLPGQVGDGPMLPSLPDGCGDATAVAPTASAQPDVVVATS
                     CGPYLLSWMSATPAGSGLPARALPTTLAAHGGAVYGGTAGSGLWRFDGSSWRTDPVGG
                     NVGTDGVGTGTVQTIRSFGGALWVGMAEGLYARGSGPWAEVSAGLPSAGFVSALAGNA
                     STAYAGLATGGVYRRPAGAPFRKDAAGLNTAPVSSLDLAGGRLWAAAGGRGFAVKRDG
                     GWAPENGGLPPNAAVTVVRGGVNPDSGGAALVVGTDGHGFYRADLFAKARTLPVVLDV
                     VGQGGARFLSELVVGSRASTQRSCTVTFTPSPGFAGEGGDLAPKSATLTLPAGRELRA
                     ADALAFLRSLGLAIPVATAAAPVAGSVSLRSETPGVVDEAYLVARTYTRGASGGTFGL
                     FYDAPSDLDAAEEVATIYGLRSVAGGARSNLAAVHLPGRGSEPIELSVQLYGEDGVAA
                     GEPLVRSLAPGEWTQWNGVLGLAGLPDGSYGFARIRRTKGEGAFAAYGVVNDARTSDG
                     SYLPAFRPGGLSAVRTLVVPVVLDVAGEADSRYTTEVTIVNDGTIATPVDLVYRPAPG
                     FGTVGGVAQVTLELAPRQQVTLPDVLAYLRAHGMQIPDGATAPQAGTLAVTFRWLTEL
                     DAPSTVVLARTSTPNPDAAVGGTFGLFYPAVAKGGGARTTALVPGLAQDEGVRSNLAV
                     VNVGGGSELPVTLEARLYDADTGAPVGSPLTVRLAPGDWHQWSRVREAAGAPSTTKRF
                     TAVVRRTSGDDTFLAYGVLNDAVTSDGSFQAMIPADLY"
     gene            4758..4832
                     /locus_tag="PLP50_12680"
     tRNA            4758..4832
                     /locus_tag="PLP50_12680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4790..4792,aa:Gly,seq:gcc)
     gene            4902..5885
                     /locus_tag="PLP50_12685"
     CDS             4902..5885
                     /locus_tag="PLP50_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPA52445.1"
                     /translation="MKAMLLDAPGGPLRLAEVPDPEPGPGQLLLRVRACAVCRTDLHV
                     VDGDLREPKLPLVLGHEIVGVVEAAGEGGSLFAKGDRVGVPWLGATCGRCRYCLEGAE
                     NLCDEARFTGYHRDGGFAELAVADERFCFPIPAELGDVEAAPLLCAGLIGHRTLRMAG
                     DPQKVGIWGFGAAAHLVAQVARYEGREVFAFTRPGDSWAQEFARSLGAVWAGGSDELP
                     AEPLDAALVFAPIGALVPAALRSVRKGGVVVCGGIHMTDLPSFPYELLWGERVVRSVA
                     NLTRRDGEEFLAVAARAGVKSQVRTYPLSAVNEALEDLREGRLEGAAVVVP"
     gene            complement(5902..6414)
                     /locus_tag="PLP50_12690"
     CDS             complement(5902..6414)
                     /locus_tag="PLP50_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52446.1"
                     /translation="MKVRLLHTRDVSPGHVRSAFSGLAPPLVPGEALPFRASLSATYD
                     RSRAQYDAVSVLEAVPEPETGWMNLLLVEADLFVPALTYVFGLSHVGGRRGVLSLSRL
                     KPPEESAVSEELLLRRLAIEVAHELGHAAGLVHCAVGDCAMHRSLWPESIDLKNPAYC
                     LSCRALFPAD"
     gene            complement(6411..7751)
                     /locus_tag="PLP50_12695"
     CDS             complement(6411..7751)
                     /locus_tag="PLP50_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA52447.1"
                     /translation="MSAPVKLMIVDDEPHLRSSLTFWFKEEGYDVLSAGSGREALATL
                     LREAPQILLVDIKMPGMDGLEVLRKVRETAPETTVIIMTAYASVETAIQALKDGAYDY
                     IVKPFDPEAISRLVKKAAERYTLLSENRELKARLEAAEPALVTAGGGPMAKVLDLVDQ
                     VAPTDTSVLVTGESGTGKELVARLIHARSPRRFGPLSVVNCGALSEGVLESELFGHEK
                     GAFTGAVGRRRGKVELAHEGTLFLDEIGDVPPKVQVDLLRVLQEKQVVRVGGNQTIPV
                     DFRLVTATHRDLEDEVAAGRFRQDFFFRIHVFAIELPSLRERPADIPVLAQHFLARFA
                     SAMNRRIGGFSPGALAAMADYPWPGNVRELQNAIERAVVLCKGDSIEAAHFPFTTNGH
                     VAAATSLTLASAEEAHIRKVLATMSFNVAQAARALEIDRVTLYNKMRKYGIDRP"
     gene            complement(7751..9358)
                     /locus_tag="PLP50_12700"
     CDS             complement(7751..9358)
                     /locus_tag="PLP50_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52448.1"
                     /translation="MQRLRANSLAVRLTGTLVAALAALLLVSWFVSLSLQRRFSESTA
                     RYSALALSETLTGGLHGMMLANDREGLESSVRAVAKAQPNIRVRVFNKEGSIVYSSIP
                     QEAGRKVDTTSEACFKCHAEGQPIEKLPPGDRTRTFQLDGVRTLGVIRPIENEPSCST
                     AACHAHPPEKRLLGVVDVSIQMVRQEQVRRQTMALMAVTGVATLLLVAAVVVLVVRRA
                     VHRPVRHLADVLGALGGGDYTARYDDEPIAEFAYLGNHLNRMAGDLQKANAELVDWAQ
                     TLERRVEEKTGELKAAQAQMLRVERMASLGKLAAVVAHEINNPLASVVTYSKLLLRRF
                     TQKGGPKPGDDSEKILEAIASESARCGEIVSNLLLFARRTGSRMEPTDLNKLVDRSLF
                     LLKHKMDLAQVATAEELAPDLPQVLCDPSQIEQAILALAINAIEAMPGGGTLSVLTAP
                     VGDRGARIEIADTGVGMEEDVKRQIFEPFFTTKGDGEGKGLGLGLAVVYGIVQRHGGA
                     IDVESAPAAGTRFVLTLPGTATPGEES"
     gene            complement(9376..10959)
                     /gene="hybB"
                     /locus_tag="PLP50_12705"
     CDS             complement(9376..10959)
                     /gene="hybB"
                     /locus_tag="PLP50_12705"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HPA52449.1"
                     /translation="MPKLALPRLTFWRGVLLAILAAGTYATFLRVFKGLAGSTNLTDA
                     FPWGIWIGFDILCGVALAAGGFTISATVYIFNLEKFRPIVRPAILTAFLGYVLVVVAL
                     LFDLGRPWNVWHPLVMWNPHSVMFEVGWCVTLYTTVLALEFSPVVFERFKMTKTLHVL
                     HVIMPILVVLGVLLSTLHQSSLGSFYLIVPTKLHALWYSPLLPVYFFLTALTLGCAMT
                     IVESFLSHRAFGKELEMELLVPLGRVALLILGVTAVIRFADFRHRGILGLPFAPTYES
                     RLFLAEMIIGVLAPALMLALPKVRQHRTGLFLSALLIVMGVVMNRLNVSITGMERSSG
                     EIYVPAFTEIAITLSVVGVGFLVFALAVKYLPVFPEHEMKDARPPVAPDALHVAAQPI
                     RSLPMPAIAVVALAFVAAGALGADGVRRREVVPGPPAPAGTPALAAALASFEAPAEMT
                     FPQAPDSPGPVSFRHASHVDASAPDCLSCHGNSYSFLGRTQAPPAGLMHDTKHCGGCH
                     DGTSAFSVEDDCTACHKSE"
     gene            complement(10970..11746)
                     /locus_tag="PLP50_12710"
     CDS             complement(10970..11746)
                     /locus_tag="PLP50_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA52450.1"
                     /translation="MLNTQAKGLLLDTTRCIGCGACALACKERNGLPKTADVVLEDKL
                     SDKTFSTVNQVGNRYVRRMCMHCATPSCASACPVAALEKTAAGPVVYHESKCMGCRYC
                     MIACPFGIPKYEWEKKAPLVRKCDLCADRLAKGLKTACATACPTGATTFGDREALVAE
                     ARARFAANPGRYREHIYGFEEVGGTSVLLISDAKPEELGYPAGLDGTPPPHLTWRVLN
                     IIPDIVMTGAVLLGGIYWIRNRRDEVEAAEGHHDASTKGH"
     gene            complement(12079..>12581)
                     /locus_tag="PLP50_12715"
     CDS             complement(12079..>12581)
                     /locus_tag="PLP50_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010370003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA52451.1"
                     /translation="DRYNGGGFIPDRMIEMLSRTTLAFWARRGVDAMRTPGFAHDGPK
                     AMLVNSYSASGGDALPYFFRKEKLGPIVGTRTWGGLIGLSGNAEFVDGGSVDVPTFRI
                     YDHDGKWVVENEGVAPDVEVFDVAERRLDGGDPSLEKGVEILIQELAKQPARRSEPPA
                     APRLND"
BASE COUNT         1676 a         4269 c         4794 g         1842 t
ORIGIN      
        1 tgacgggaaa cccgaggagg cgacgtggcg gatccgaaga agcccgaccg caaggtgccg
       61 tcgaacccgt acgtcacggg accgacgaga atcctcgacg cctcctcgtc cggcccctcc
      121 gcccggggtg gcggagaggc gccgcgcccg ggggtgcgcc ccgagctcgt caccgacggg
      181 atgaaggccc atcggcgcga gatcgagaag cgcaagcgcc aggcatcgat cgagaagatg
      241 cacctcgaga tgcagacggc cgacgagcgg aaggcgatct ggaagtggac gttccgggtc
      301 gtcctcgtcc tcgtcatcct cttcgcttac tggaaggtgc aggacgccta cgggaaccag
      361 tggccgatgt gggagacctg gatcgtcctc gcgatcgtcc tcttcgcggg gatcggctgg
      421 ctgctatggt acctcaacaa gaccgagctg tgatgttcgc gggggtttcg cgatcccccc
      481 gcaccccccc cggggagatg ttcgcggggg aacgccggtt cccccggtct cgcgcctgaa
      541 gcggtcgcgc ggctgcggtc agtcggcgtc gaaggggctg cgggggcggc ccggcggctc
      601 cttctgggcc ttctgaagcg cctcgcggaa ggtcttctcc aggatctcgc tcttgtgctt
      661 ctcccgctcc acgttcgcgg cgaagaggtc ctccgcgcgc tccttgcggc gagcgatctc
      721 cgcggccgcc tcctcgatcg agccgagctt ccgcttcgcg gccgccggct cgtgagagag
      781 gaccgcgccg gtctcgcggt cgaccttcag cgtcgagtcg cagtcggggc actggattac
      841 gaagacgtcg cccatgggtc gattatcctc gaccgatgcc gtccggatgg aaggagcgcc
      901 tctcggagct cgggatctcg cgccggcggc gtccggccgc cgcgaggccg gggccctctc
      961 ggccggcgac gacgccgaag gggcgtgccg gcgaggacga agcggcccgc ttcctcgagg
     1021 cgcaggggct cgagctcctc gcgcggaacg tccgcgcggg agagggcgag ctcgacctcg
     1081 tggcggcgga gggccgctgc gtcgtcttcg tcgaggtgaa atggcgacgc gacgccagcc
     1141 gtggggctcc ggccgaggcg gtcactcccg tgaagaggcg gcggctcctg tcggcggccc
     1201 gcgccttcct cgcggagcat ccctcccggg cgcgccgcga cgtgaggttc gacgtcgtgg
     1261 cgatcgaggg cgaggccggg aagatcgact ggatccgagg agcgttcgat gcgacggact
     1321 gaacgggggc ccaggatgcg gagctggcga gcggcggcgc tcgtcgcggc ggcgacggtc
     1381 ttcggactcg ccccttcggc ggccgaggcc ggcctgcggc tcgaatcgga cggcgggtcc
     1441 ggggcggacg tccgcgcgtt cgcctcgggc gggggcgcct tctgggccgg gacgcggagc
     1501 ggagtcctcc gggggacctc cctcgacgcg gcgtggcgct tcgacggtct ctccgggaag
     1561 ccggtctcgt cggtcgcgtc tctcggggac gaggtctggg cggcgacggg cgaggagctc
     1621 tggcggcgtt ccgcgaacgg gagctggagc ctcgaggcgc tcccgtcgag cgtcgccttc
     1681 ccgacgacgc tcgccgtcga cgcttcgggg acgctctggg tcggcgggct cggcgtctgg
     1741 cgccgctccg gcgcttcgtg ggtggccgct ccgtctcccg gcgtcgggat cgtcacggcg
     1801 atgctcgcgg accccgcggg cctcgtcgtc ggcctctcgg ccgggagcgc ggcccgatgg
     1861 aacggctcgg gctgggcgcc cctcaacggc ggcgtcggcc cgggagaggc gatccgggcg
     1921 ctcgcctcgt tcggcgggac gctctgggcc gggacgaacg taaccctcta ctggtggggc
     1981 gtcggcgcct gggtggcgga cggcccgttc ggcgggcacg acgtccgcgc cctcacgacg
     2041 tggggcggcg cgctgcgcgc cgcgacggcc gacgcgggcg tcctcgtccg gtcgggcagc
     2101 gcctggacgg cggcttcgac cggcatcctg ccccgcggtg ttcaggcgtt cctcgtcgcc
     2161 ggcaccgagc tcctcgctgg gacctcgggc ggccccgtct accgcttctg gaacgggacc
     2221 tggcatccgt ccgcggtcac cccggtcggg gcgggcgtgg cggcggacgc cttcgcgtat
     2281 ccggcagaag cggcgggttc gcaggccggt gcggcggtcg gcgtgacgcg cggcggaggg
     2341 gcggactacc tgcttcggcc ctgctgcctg ccgggccagg tgggggacgg gccgatgttg
     2401 ccctcccttc ccgacggctg cggcgacgcg acggcagtgg cgccgacggc gtcggcgcag
     2461 cccgacgtcg tcgtcgccac gagctgcggg ccgtacctgc tgagctggat gtcggcgact
     2521 cccgccgggt cgggcctgcc ggcccgcgcc ctcccgacga cgctggccgc tcacggcgga
     2581 gccgtctacg gagggacggc cgggagcggc ctctggcggt tcgacggctc ctcgtggagg
     2641 accgatcccg tcggagggaa cgtcggcacc gacggcgtcg gtacggggac cgtccagacg
     2701 atccggtcgt tcggcggcgc gctctgggtc ggcatggccg aagggctcta cgcgcgcggc
     2761 agcgggccgt gggccgaggt ctcggccggc ctcccgtccg ccggcttcgt ctccgcgctc
     2821 gccgggaacg cgtcgaccgc gtacgcgggc ctcgcgaccg gtggcgtcta tcggcgaccg
     2881 gcgggagcgc ctttccggaa ggacgcggcg ggcctcaaca cggcgccggt ctcctcgctc
     2941 gacctcgccg gcggccgtct ctgggccgcg gccggcggcc gcggcttcgc cgtgaagcgc
     3001 gacggaggct gggctcccga gaacggcggg ctgccgccga acgccgccgt caccgtcgtc
     3061 cgcggaggcg tgaaccccga ctcgggaggg gcagcgctcg tcgtcggcac ggacggccac
     3121 ggcttctacc gcgcggacct cttcgcgaag gcgcggacgc tccccgtcgt cctggacgtc
     3181 gtcggccagg ggggcgcgcg cttcctctcg gagctcgtcg tcgggtcgcg cgcctcgacg
     3241 cagaggagct gcaccgtcac gttcacgccg tcaccgggct tcgccgggga agggggcgac
     3301 ctggcgccga agtcggcgac gctgacgctc cccgcggggc gcgagctgcg cgccgccgac
     3361 gcgctcgcgt tcctccgctc cctcggcctg gcgatccccg tcgccacggc tgccgcgccg
     3421 gtcgccggga gcgtctcgct ccggtcggag acgcccgggg tcgtcgacga ggcctacctc
     3481 gtggcgcgga cctacacgcg gggcgcctcg ggcgggacgt tcggcctctt ctacgacgcc
     3541 ccgtccgacc tcgacgcggc ggaggaggtc gcgacgatct acggcctccg gtcggtcgcc
     3601 ggcggggcgc ggtcgaacct cgccgccgtc cacctgccgg ggcgcgggag cgagccgatc
     3661 gagctctccg tgcagctcta cggcgaggac ggcgtggcgg cgggcgagcc gctcgtccgt
     3721 tcgctcgcgc cgggggagtg gacgcagtgg aacggcgtcc tcggcctcgc gggccttccc
     3781 gacgggagct atggcttcgc gcgaatccgc cggacgaagg gagagggcgc cttcgcggcc
     3841 tacggcgtcg tgaacgacgc gcggacgtcc gacgggagct acctccccgc gttccgtccc
     3901 ggcggcctct cggccgtccg gacgctcgtc gtcccggtcg tcctcgacgt tgcgggcgag
     3961 gcggactcgc gctacacgac cgaggtgacg atcgtgaacg acgggacgat cgcgacgccc
     4021 gtcgacctcg tctaccggcc ggcgcccggc ttcgggacag tcggcggcgt cgcgcaggtg
     4081 acgctcgagc tggcgccccg ccagcaggtg acgctccccg acgtcctcgc ctacctgcgc
     4141 gcccacggga tgcagatccc cgacggcgcg acggcgccgc aggccggcac gctcgccgtg
     4201 acgttccgct ggctgaccga gctggacgcg ccgtcgacgg tcgtcctcgc gcgcacgtcg
     4261 acgccgaacc cggacgcggc cgtcggcggc acgttcgggc tcttctaccc ggccgtggcg
     4321 aagggcggcg gcgcccggac gacggcgctc gtcccgggcc tcgcgcagga cgagggggtc
     4381 cgctcgaacc tcgccgtcgt gaacgtgggc ggtggctcgg agctcccggt cacgctcgaa
     4441 gcgcggctct acgacgccga cacgggcgct ccggtcggca gcccgctcac ggtgcgcctc
     4501 gcgcccggcg actggcacca gtggtcccgg gtccgggagg cggcgggcgc gccgtcgacg
     4561 acgaagcgct tcacggcggt cgtgaggcgg acctcggggg acgacacgtt cctcgcctac
     4621 ggcgtcctga acgacgccgt cacttccgac ggcagcttcc aggcgatgat cccggcggac
     4681 ctgtattgag cggaggagga gggggaccac gccttgacga gagcgccctt cttcgctatc
     4741 ttccgccggt cccgtgagcg ggactagctc agtggtagag cgctaccttg ccaaggtaga
     4801 tgtcgcgagt tcgaccctcg tgtcccgctc catcgaactc gaccgggggg gcctcacggc
     4861 cctcccgttt tcgtttcagg cgccgcactg aggagggacc cgtgaaggcg atgctgctcg
     4921 acgctccggg gggcccgctt cgtctcgccg aggttccgga ccctgagccc ggacccggcc
     4981 agctcctcct gcgcgtccgc gcctgcgcgg tctgccggac cgaccttcac gtcgtcgacg
     5041 gggacctccg ggagccgaag ctcccgctcg tcctggggca cgagatcgtc ggcgtcgtcg
     5101 aggccgccgg cgaggggggc tcgctcttcg cgaaggggga ccgcgtcggc gtcccgtggc
     5161 tcggggccac ctgcgggcgc tgccggtact gcctcgaggg agcggagaac ctctgcgacg
     5221 aggcccgctt caccggctac caccgcgacg ggggcttcgc cgagctcgcc gtggccgacg
     5281 agcggttctg cttcccgatc cccgccgagc tcggggacgt ggaggcggcg ccgctccttt
     5341 gcgccggcct gatcgggcac cggacgctcc ggatggcggg cgacccgcag aaggtcggga
     5401 tctggggctt cggggccgcc gcgcacctcg tcgcgcaggt ggcccgctac gaggggcgcg
     5461 aggtcttcgc cttcacccgt cccggcgact cctgggcgca ggagttcgcc cgttccctcg
     5521 gcgcggtctg ggccggcggc tccgacgaac tcccggccga gcctctcgac gccgcgctcg
     5581 tcttcgcccc gatcggggcg ctggtgccgg ccgccctccg ctcggtccgc aaggggggcg
     5641 tcgtcgtctg cggcggcatc cacatgaccg acctcccgtc gttcccgtac gagctcctgt
     5701 ggggcgagcg cgtcgtccgc tcggtcgcga acctgacgcg ccgggacggt gaggagttcc
     5761 tcgcggtcgc ggcgcgggcg ggcgtgaaga gccaggtgcg cacctacccg ctctcggcgg
     5821 tgaacgaggc gctcgaggac ctgcgcgagg ggcgcctcga gggggccgcg gtcgtcgtcc
     5881 cctgacgggg ccggacgcgg cctaatccgc gggaaagagc gcgcggcagg agaggcagta
     5941 ggcggggttc ttcaggtcga tgctctccgg ccagagagag cggtgcatcg cgcagtctcc
     6001 gacggcgcag tggacgaggc cggccgcgtg cccgagctcg tgggcgacct cgatcgccag
     6061 gcgccggagg aggagctctt ccgagacggc ggactcctcc ggcggcttga ggcgcgagag
     6121 cgacaggacg ccccggcgtc cgccgacgtg ggagaggccg aagacgtagg tgagggcggg
     6181 gacgaagagg tcggcctcga cgaggaggag gttcatccag cccgtctcgg gctcggggac
     6241 ggcctcgagg acggagacgg cgtcgtactg cgcccggctg cggtcgtagg tggcggagag
     6301 ggacgcgcga aaggggagcg cctcgcccgg gacgagcggg ggcgcgaggc cgctgaaggc
     6361 gctcctcacg tgccccggcg agacgtctcg ggtgtggagg agccggacct tcacgggcgg
     6421 tcgataccgt acttccgcat cttgttgtag agggtgaccc ggtcgatctc gagggcgcgg
     6481 gcggcctggg cgacgttgaa cgacatcgtc gccaggacct tccggatgtg ggcctcctcg
     6541 gcggaggcga gcgtcagcga cgttgcggcc gcgacgtggc cgttcgtcgt gaacgggaag
     6601 tgggccgcct cgatcgagtc ccccttgcag aggacgacgg cccgctcgat ggcgttctgg
     6661 agctcgcgga cgttccccgg ccaggggtag tcggccatcg cggcgagcgc gccgggcgag
     6721 aagccgccga tccggcggtt catggcgctc gcgaagcgcg cgaggaagtg ctgcgcgagg
     6781 accgggatgt cggccgggcg ctcccgcagg gacgggagct cgatcgcgaa gacgtggatc
     6841 cgaaagaaga agtcctgccg gaagcggccc gcggcgacct cgtcctcgag gtcgcggtgc
     6901 gtcgcggtga cgagccggaa gtcgaccggg atcgtctggt tcccgccgac gcggacgacc
     6961 tgcttctcct ggaggacgcg caggaggtcg acctggacct tcggcgggac gtcgccgatc
     7021 tcgtcgagga agagcgtccc ctcgtgcgcg agctccacct tcccgcggcg ccggccgacg
     7081 gccccggtga aggccccttt ctcgtgcccg aagagctcgc tctcgagcac cccctcggag
     7141 agggcgccgc agttgacgac gctgagcggc ccgaagcggc gcggactccg ggcgtggatg
     7201 aggcgggcga cgagctcctt gccggtgccg ctctcgccgg tgacgaggac gctcgtgtcg
     7261 gtcggcgcga cctggtcgac gaggtcgagg accttcgcca tcggtccgcc gcccgccgtg
     7321 acgagggccg gctcggccgc ctcgagccgc gccttcagct cgcggttctc cgagaggagc
     7381 gtgtagcgct cggccgcctt cttcacgagg cgcgagatcg cctccgggtc gaacggcttg
     7441 acgatgtagt cgtacgcgcc gtccttgagg gcctggatcg cggtctcgac ggaggcgtag
     7501 gccgtcatga tgatgacggt cgtctcggga gcggtctcgc gcaccttgcg gaggacctcg
     7561 agcccgtcca tgccgggcat cttgatgtcg acgaggagga tctgcggcgc ctcgcgcagg
     7621 agcgtcgcga gcgcctcgcg cccgctcccg gcggagagga cgtcgtaccc ctcctccttg
     7681 aaccagaacg tgagcgagct ccgcaggtgc ggctcgtcgt cgacgatcat cagcttgacg
     7741 ggcgcggaca tcaggactcc tctcccgggg tggcggtccc cggcagggtc aggacgaagc
     7801 gggtgccggc ggcgggagcg ctctcgacgt cgatcgcgcc cccgtgccgc tggacgatgc
     7861 cgtagacgac cgcgaggccg aggccgagcc ccttcccctc gccgtccccc ttcgtcgtga
     7921 aaaagggctc gaagatctgc cgcttcacgt cctcctccat cccgacgccg gtgtcggcga
     7981 tctcgatccg cgccccgcga tccccgacgg gtgcggtgag gacggagagc gtcccgccgc
     8041 cgggcatcgc ctcgatcgcg ttgatggcga gcgcgaggat cgcctgctcg atctgggacg
     8101 ggtcgcaaag gacctgcggg aggtccgggg ccagctcctc ggcggtggcg acctgggcga
     8161 ggtccatctt gtgcttgagg aggaagaggg agcggtcgac gagcttgttc aggtcggtcg
     8221 gctccattcg cgagccggtg cggcgcgcga agaggagcag gttcgagacg atctcgccgc
     8281 agcgggccga ctcggaggcg atcgcctcga ggatcttctc gctgtcgtcc cccggcttcg
     8341 gccctccctt ctgggtgaat cggcggagga ggagcttcga gtaggtgacg acgctcgcga
     8401 gcgggttgtt gatctcgtgg gcgacgaccg ccgcgagctt gccgagggag gccatccgct
     8461 cgacgcggag catctgcgcc tgggcggcct tcagctcgcc cgtcttctcc tcgacgcggc
     8521 gctcgagcgt ctgggcccag tcgacgagct cggcgttcgc cttctgcagg tcgcccgcca
     8581 tccggttcag gtggttcccg aggtaggcga actcggcgat cggctcgtcg tcgtaccggg
     8641 cggtgtagtc gccgccgccc agcgcgccga ggacgtcggc gaggtggcgg acggggcggt
     8701 ggacggctcg cctcacgacg aggacgacga cggcggcgac gaggaggagc gtggcgacgc
     8761 cggtgacggc catcagggcc atcgtctggc gccgcacctg ctcctgccgg accatctgga
     8821 tggagacgtc gacgacgccg aggagccgct tctcgggcgg gtgcgcgtgg caggcggccg
     8881 tcgagcagga gggctcgttc tcgatcggcc ggatgacgcc gagcgtccgg acgccgtcga
     8941 gctggaacgt gcgggtccgg tcgcccggag ggagcttctc gatcggctgc ccctcggcgt
     9001 ggcacttgaa gcaggcctcc gaggtcgtgt caaccttccg gcccgcctcc tgcgggatcg
     9061 aggagtagac gatcgagcct tccttgttga agacccgcac gcggatgttc ggctgcgcct
     9121 tcgcgaccgc cctcacggag ctctcgagcc cctcgcggtc gttcgcgagc atcatgccgt
     9181 ggagcccgcc cgtgagcgtc tccgagaggg cgagcgcgct gtagcgcgcc gtcgactcgg
     9241 agaacctccg ctgcagcgag agcgacacga accaggagac gaggaggagg gcggcgagag
     9301 cggcgaccag ggtcccggtc aggcggaccg cgagggagtt ggcccggagc ctctgcatcg
     9361 ccgctcccat ccttcctact cggacttgtg gcaggccgtg cagtcgtcct cgacggagaa
     9421 cgccgacgtg ccgtcgtggc agccgccgca gtgcttcgta tcgtgcatca ggcccgccgg
     9481 gggcgcctgg gtccggccga ggaacgagta gctgttcccg tggcaggaga ggcagtcggg
     9541 ggcggaggcg tcgacgtggg aggcgtggcg gaacgagacg gggccggggc tgtcgggcgc
     9601 ctgggggaac gtcatctcgg cgggcgcctc gaagctcgcg agcgcggcgg cgagggccgg
     9661 cgtccccgcg ggagccggcg ggccggggac cacctcgcgg cggcggactc cgtcggcgcc
     9721 gagggcgccg gccgcgacga acgcgagcgc gacgacggcg atcgccggca tcgggaggga
     9781 gcggatcggc tgcgcggcga cgtggagcgc gtccggcgcg acgggcgggc gggcgtcctt
     9841 catctcgtgc tcggggaaga ccgggaggta cttcacggcg agcgcgaaga cgagaaagcc
     9901 gacgccgacg accgacaggg tgatggcgat ctcggtgaac gccgggacgt agatctcgcc
     9961 cgaggagcgt tccatgccgg tgatcgagac gttcaggcgg ttcatcacga cgcccatgac
    10021 gatgagcagc gccgagagga agagcccggt ccggtgctgg cggaccttcg gcagcgcgag
    10081 catgagggcc ggggcgagga cgccgatgat catctcggcg aggaaaagcc gcgactcgta
    10141 ggtcggggcg aacggcaggc cgaggatccc gcggtgccgg aagtcggcga agcggatcac
    10201 cgccgtgacg ccgaggatga ggagcgcgac ccgcccgagc gggacgagga gctccatctc
    10261 cagctccttg ccgaacgcgc ggtgcgagag gaacgactcg acgatcgtca tcgcgcagcc
    10321 cagcgtgagg gcggtgagga agaagtagac ggggaggagc ggcgagtacc agagggcgtg
    10381 gagcttcgtc gggacgatca ggtagaacga cccgagggac gactggtgga gcgtcgagag
    10441 gaggacgccg aggacgacga ggatcggcat gatcacgtgc aggacgtgga gcgtcttcgt
    10501 catcttgaag cgctcgaaga cgaccgggct gaactcgagc gccaggaccg tcgtgtagag
    10561 cgtcacgcac cacccgacct cgaacatgac cgagtggggg ttccacatga cgagcgggtg
    10621 ccagacgttc caggggcgcc cgaggtcgaa gaggagcgcc acgacgacga ggacgtagcc
    10681 gaggaacgcc gtcaggatcg cgggcctcac gatcggtcgg aacttctcga ggttgaagat
    10741 gtagaccgtg gcgctgatcg tgaagccgcc ggcggcgagg gcgacgccgc agaggatgtc
    10801 gaacccgatc cagatgcccc aggggaaggc gtcggtcagg ttcgtggagc cggcgagccc
    10861 cttgaagaca cggaggaacg tggcgtaggt cccggcggcg aggatcgcca ggaggacgcc
    10921 gcgccagaac gtgaggcggg gaagcgcgag cttcggcatg tcgctctcct cagtgcccct
    10981 tcgtcgaggc gtcgtggtgg ccctcggccg cctcgacctc gtcgcggcgg ttgcggatcc
    11041 agtagatgcc gccgaggagg acggcgccgg tcatgacgat gtcggggatg atgttgagga
    11101 cccgccacgt caggtgcggc ggcggggtcc cgtcgaggcc ggcggggtag ccgagctcct
    11161 cgggcttcgc gtcggagatc aggaggacgg acgtcccgcc gacctcctcg aagccgtaga
    11221 tgtgctcgcg gtagcgcccc gggttcgcgg cgaagcgcgc gcgggcctcg gcgacgaggg
    11281 cctcccggtc gccgaacgtc gtcgcccccg tcgggcaggc ggtcgcgcag gccgtcttca
    11341 gccctttcgc gaggcggtcg gcgcacaggt cgcacttccg cacgagcggc gccttcttct
    11401 cccactcgta cttcgggatg ccgaaggggc aggcgatcat gcagtagcgg cagcccatgc
    11461 acttggactc gtggtagacg accgggccgg ccgcagtctt ctcgagggcg gcgaccgggc
    11521 aggccgacgc acacgacggc gtcgcgcagt gcatgcacat gcgccgcacg taccgattcc
    11581 cgacctggtt caccgtcgag aacgtcttgt cggacagctt gtcctcgagg acgacgtccg
    11641 cggtcttcgg gagcccgttt cgctccttgc aggcgagcgc gcacgcgccg cacccgatac
    11701 agcgcgtcgt gtccaggagc agccccttcg cttgcgtatt caacatgggc ctctcgtacc
    11761 cccccgggcg aacttcaggt ccgccccgtt gagaatttcg caagcaccgg gccaggttgt
    11821 tgagtttctc gcaagctatt gctgtgtatg cgtttgagtc gtgcctttcg gttggggtcg
    11881 agttcgagag ttgcgaattt caacgtcgcg atttcgctgc tcggagctaa gtcatccatt
    11941 tgcagtagat aggcgtggag cgcgatgtgt cgcgttttga ggctgtacgt tgagaaattc
    12001 tacgcactgg ggcgaattcc aacacgaaag gcccggcggc gtggagtatt tccacgccgc
    12061 cgggggcgga ggtcggactc agtcgttcaa gcggggggcc gcgggaggct cggagcgcct
    12121 cgccggctgc ttcgcgagct cctggatgag gatctccaca cccttctcga ggctcgggtc
    12181 gcctccgtcg agccgtcgct cagcgacgtc gaagacctcg acgtcgggag cgaccccctc
    12241 gttctcgacg acccacttcc cgtcgtggtc gtagatccgg aacgtcggga cgtcgacgct
    12301 gccgccgtcg acgaactcgg cgtttcccga gaggccgatc agcccccccc acgtcctcgt
    12361 cccgacgatc gggccgagct tctccttgcg gaagaagtac gggagggcgt cgccgcccga
    12421 ggcggagtag gagttgacga gcatcgcctt cgggccgtcg tgggcgaagc cgggggtgcg
    12481 catggcgtcg acgccccgcc gggcccagaa agcgagcgtc gtcctcgaga gcatctcgat
    12541 catccggtcg gggatgaacc cgccgccgtt gtagcggtcg t
//
LOCUS       DAOONL010000094        12191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5777_length_12191_cov_19.575313, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000094 DAOONL010000000
VERSION     DAOONL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5777_length_12191_cov_19.575313"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2526
                     /locus_tag="PLP50_12720"
     CDS             <1..2526
                     /locus_tag="PLP50_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA52452.1"
                     /translation="RWASSDNAGKVVYELGGELRLWDVAAKAERAIPITVPSDGLASR
                     PSRISAEKNIERAGLSPKGERALFVARGDVFTAPIEKGPTRNLTRSSNAHDKHAAWSP
                     DGKTILFVSDRSGEEQLYTVPQDGSAKPTQLTTTFGSMLSPPVWSPDGARVAVSDKDG
                     KLYVVTVADRKVKEIADDRFGGLFDYAFSPDGAHLAFSMGEHTGMRVLHVWSAADGKV
                     RKVTSELFSSFQPAWDPEGKVLWFLSNREFAPQISDIEWNFAGNRRTGVFGLALRKDV
                     ADPFAPESDEVTAGDAKKDDEKDATAADGKKGDGKDAKKDEAKAPKPVVIDWDGLAER
                     VVRVPVEADNLDGLEATKTALLFTKEGASFYGRDSDREPALVIFDLKEREESDLVANV
                     DGWALSRDGTKVLVRGGGKEKGWKLWDVKPKAKEPKSVSTKELAVDRVPAEEWANVFD
                     EVWRRYRDFFYVRNMHGYDWKAIGERYRALLPHVSHRSDLTYLLGEMVAELNVGHAYV
                     EEGDIERPARAKVGLPGARFELDGKAGLYRIAKVFRGHNEEPKYRAPLTEVGVDARVG
                     DYVLAVDGEPLAGTDNPYRLLQHKTHPVTLTLNAKPTVEGARKVTYRPVENEASLLYL
                     DWVLGNVEKVSKLSNGRVGYLHVPDMGAPGIYEFLKWYYPQIRKEGLVVDVRSNGGGN
                     VSQWILERLDSKLLGTRFGFSSDLPMTYPYAVFHGHLAALISETSASDGDIFPARFRK
                     AGLGPLIGKRTWGGVVGISGRGPLIDGGQVFVPQQATNDVDGAYIIEGEGVAPDIEVE
                     NDPASVAAGRDPQLERGVAEVLKAIEKEPRTLPKRPADPVKTK"
     gene            complement(2551..4083)
                     /locus_tag="PLP50_12725"
     CDS             complement(2551..4083)
                     /locus_tag="PLP50_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA52453.1"
                     /translation="MTTAGDDPDRRRGRRPGRIGVRLPALLVVAAALLPSGACRERPV
                     RRSVEVLVPVLPESLDPYSDPRLVSRSVFSAIYEPLVAEHEAGFRPALAQSWTNPAPD
                     TWLFRIAEGASFHDGSPVTSRDVVQAAHASRTARGSVASLADLRAIEVADARTVRFRT
                     FRAPEDFLHSVSALFIARKEGESFVGSGPYRVVARTPDRIVLARHERPSRPAPYLDEV
                     VFRRFANAAEGLRLMGRDVPIAVADPAPAMVAAARGDPRLRVVTTDSGGLTYLAIGFS
                     AGAGPLSDLRVRRALRLAIDLPALVAAGTVAGGTPVGRLVPPGSFGFDPGRRPPRRDL
                     ATARRLLEEAGHPHGFDLDLDVGPNGRRAGEALAAQASEAGIRLRVRVHRPDDFVTRI
                     EGRSPLYLYSWFVGRDAGQALRNAFHTRDASRGLGSMNRTGFSSAAVDAAFSRLTFAT
                     RAEERLTRIREVSDLLDEELPWIPLYSARESRIFPAGLDLGWRPDGLLVIADARPAGG
                     GR"
     gene            complement(4080..6287)
                     /locus_tag="PLP50_12730"
     CDS             complement(4080..6287)
                     /locus_tag="PLP50_12730"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPA52454.1"
                     /translation="MTPRLRTAALLLPLLVLLALPGTASSDEGQWPPEALATLGEARW
                     AELAARGLELGATDLWDGKGGGLLTAVLGLGGCTGAFVSDEGLFLTNHHCAFGAIQLA
                     STPEKNYLRDGFVARSRAEEVPARGGVGRVRILTRLSDVTDRVRGPKSAFPRAKDDAA
                     REAAVEKARSEIVAECEKQPDRRCAVAAFEDGRTYRLMEQTEFRDVRLVYAPPRGVGD
                     FGGEVDNFRWPRHAGDFTLLRVWAAPDGAPAAHAKENVPYRPKRWLRVSTRGVSEGDV
                     VMIAGYPGRTQRYLTPSAVENLEEWFYPLRSRTFTDLIAILETEGKKDPDTALRVSGA
                     VKGYGNGETNARGQIEGLARNGVAARARAEAKELEEFLAARKDLPADWTAAPAELEKV
                     LARDREGQERRFFLEEIERLSSYLSSALSSVRRADERRKPDLEREPGFQERDLERARQ
                     QEKDRTLSLAPEATRKALAYLVEKARAASKGAPIRALDEAFAGDADRAAIEARLAGMD
                     AATSLSDEKARLANVDATFASLSASADPYLKLAVALHADLAAIRQRRKETNGALLVWR
                     PRYLSALEALRRSKGQAIYPDANSTLRVSFATVKGYSPREAVTFTPRTSLLGVLEKAR
                     AEEPFVVPGKVLEAASAHRFGRWSDPALGSVPVGFLTDGDTTGGNSGSPTMNGKGEVV
                     GLNFDRVWENVAGDFGWRPERSRNVNVDLRYALWMMEEVDGAAHLVKELLPER"
     gene            complement(6344..7129)
                     /locus_tag="PLP50_12735"
     CDS             complement(6344..7129)
                     /locus_tag="PLP50_12735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006106539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPA52455.1"
                     /translation="MRTFVVCASLAAIAALALPAAAAVKSATVEYLDGDQLCEGFLAW
                     DDALQGKRPGVVVVHEWMGLGDHAKERARQLAALGYVAFAADIYGKGIRAKDAAEAGR
                     LAGRFKGDVPLLRSRARAALDTLAKDPRADASRLFAIGYCFGGTTALELARSGAPVAG
                     VVSFHGGLGTKDPADAKNVKGRVLVLHGAADPYVPPAEVAAFQKEMADARVDWQMVYY
                     AGAVHSFTNPAAGNDPSKGAAYDARADRRSWEAMKAFLAEEAK"
     gene            7551..10772
                     /locus_tag="PLP50_12740"
     CDS             7551..10772
                     /locus_tag="PLP50_12740"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA52456.1"
                     /translation="MKRLGLVFVLLMVAVIAAPADAQEQTGVLTGRITADGGEALPGV
                     SVEIKGPSGTLVSVTDARGDYRFPRVLPGVYTVTARLQGFQTYEASRVPVSLGDRAIL
                     NVALKISSVMETVVVTGERVQIAVGENSTTASMAAEQIALLPKGRDFTSVVAQAPGAA
                     NEAFAGGISIDGASGSENRFVIDGIDTTRPNDGLSGQSFITDFVEEIQVKSAGYSAEF
                     GGSLGGVINAITKSGTNEFHGWLGGYFSSSTLNGDRRPIAYESWPGFYRTFDKDDESR
                     FEPGFALGGPIIKDSLWFYLGYSPTLRSIERTPYQKATTAEQDITDHFFAGNIKGNIG
                     SKLVFKVSANVTPSETDGNLPALDGSTPDDADLDVVSKYPTSSYSAYADFVPTSNFYV
                     SGRVGLFETDTEITGIDAQNRMLFRNGVIPVPETDPRYRPTGFATIPDASFQSADEDN
                     WKRTSAGIDANLFVTALGSHSFKAGVQYEKIENSVSLGENGNYFEVRWGLPDRFGMGV
                     QGTYGSIGVRRFRTEGAAEASNVGLFIQDTWNVHPNLTLNIGVRTEQERIPNYGAQVD
                     PTLPKNAIEFDFEDKLAPRLGFSWDVTGKQQLKVYGSYGTYYDITKLEMPRGSFGADR
                     WIQHMYELNTLDWDTFFAACARVDNTLGQNPCPTLVGHKARDLRAPGDPHDSIDPDLQ
                     PMENEEIQLGVDYQLTKNSVVSARYVNKKLKNTIEDIGYLVFLPDGSSEEQYITGNPG
                     KGIVSGDPAGAIPPQAEAIRDYEAIELTFARRFADNFSVRAAYVYSKLSGNYSGLASS
                     DEFGRTDPNVARYFDGLVYGYDSEGRIVDDVLNTDRPHAFEVQGLYQASWGTNIGLSF
                     SWASGSPVSTDASYNGVNFFPNGRNDMGRLSGLTQTDLLLTHPFKIGGYELEVSLNVL
                     NLFDQDTVTRIDNVKYFDDICDVSAECDGSNDWYFSDLVPYDYSTYMDANGASKNPLY
                     GKPLTYQAPPDGPLRSEVRLLTRPVFRSTDGGSARALPPSSLLEVSANEAGSPVGARH
                     GSFKGITGQICGRIRSRPPRRRKVSAISSSGACFDLALRPGPL"
     gene            11043..>12191
                     /locus_tag="PLP50_12745"
     CDS             11043..>12191
                     /locus_tag="PLP50_12745"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA52457.1"
                     /translation="MNGKRLWLLVLLVPFLLAVQADAQGLPTGTLTGRVAEAEGLALP
                     GVTVTAKSPALQGTRTAVTNVNGDFVIPNLPPGEYVVAFVMSGFQNVTRNVKVSSGQQ
                     VPLNTKLSIAAVAAEAMVVAQSETVSQTAQASTTYSTELTNKLPVARQILSSVVLSAG
                     VNTNGPGGNTTISGAQSFDNVITVNGVNIQDNIRGTPTNTLVIEDAIQETTTMTSGVS
                     AEYGRFTGGVINAVTKQGGNAFSGSFRVTLNNNDWQSQGPIRQNFVDKVVPTYEATFG
                     GPILKDRLWFFGAGRYQKNDYSGALSAPLTGSFPRSDKDTRYEGKLTFTPFQNHTITG
                     SYTKGEVEQSNYYFTSFPILETNGVTYDRSLPTDLLSINYNGVLSSNFF"
BASE COUNT         1942 a         4348 c         4208 g         1693 t
ORIGIN      
        1 cgctgggcca gctccgacaa cgccgggaag gtcgtctacg agctgggcgg cgagctccgc
       61 ctctgggacg tcgcggcgaa ggccgagcgg gcgatcccga tcaccgtccc gagcgacggt
      121 ctcgcctcgc gcccgtcgcg gatctccgcc gagaagaaca tcgagcgggc cggcctctcg
      181 ccgaaggggg agcgcgccct cttcgtcgcg cgcggcgacg tcttcacggc gccgatcgag
      241 aaggggccga cgcggaacct gacccgctcc tcgaacgcgc acgacaagca cgcggcctgg
      301 tcgcccgacg ggaagacgat cctcttcgtc tccgaccgga gcggcgagga gcagctctac
      361 accgtcccgc aggacggctc ggcgaagccg acccagctga cgacgacgtt cggctcgatg
      421 ctctctccgc cggtctggtc tcccgacggc gcccgggtcg ccgtctccga caaggacggg
      481 aagctctacg tcgtgaccgt ggcggaccgg aaggtgaaag agatcgccga cgaccgcttc
      541 ggcggcctct tcgactacgc cttctcgccc gacggggcgc acctcgcctt ctcgatgggc
      601 gagcacacgg ggatgcgcgt cctccacgtc tggagcgccg cggacggcaa ggtgcggaag
      661 gtcacaagcg agctcttctc ctcgttccag cccgcctggg acccggaggg gaaggtcctc
      721 tggttcctgt cgaaccgcga gttcgcgccc cagatctcgg acatcgagtg gaacttcgcc
      781 ggcaaccgcc ggaccggcgt cttcggcctc gcgctccgga aggacgtcgc cgacccgttc
      841 gcccccgaga gcgacgaggt gaccgcggga gacgcgaaga aggacgacga gaaggacgcg
      901 acggccgccg acgggaagaa gggcgacggg aaggacgcga agaaggacga ggcgaaggcg
      961 ccgaagccgg tcgtcatcga ctgggacggc ctcgccgagc gcgtcgtccg cgtgccggtc
     1021 gaggcggaca acctcgacgg cctcgaggcg acgaagacgg cgctcctctt cacgaaggaa
     1081 ggggcctcgt tctacgggcg cgactccgac cgcgagccgg cgctcgtgat cttcgacctg
     1141 aaggagcgcg aggagtccga cctcgtcgcg aacgtcgacg gctgggccct ctcccgcgac
     1201 gggacgaagg tcctcgtccg cggcggcggc aaggagaagg gctggaagct ctgggacgtc
     1261 aagcccaagg cgaaggagcc gaagagcgtc tcgacgaagg agctcgccgt cgaccgcgtc
     1321 cccgccgagg agtgggcgaa cgtcttcgac gaggtctggc gccggtaccg cgacttcttc
     1381 tacgtccgca acatgcacgg ctacgactgg aaggcgatcg gcgagcgcta ccgggcgctc
     1441 ctgccgcacg tctcccaccg ctccgacctg acgtacctcc tcggcgagat ggtcgcggag
     1501 ctgaacgtcg gccacgcgta cgtcgaggag ggggacatcg agcgcccggc gcgcgcgaag
     1561 gtcggcctgc cgggcgcgcg cttcgagctc gacgggaagg cgggcctcta ccggatcgcg
     1621 aaggtcttcc gcggccacaa cgaggagccg aagtaccgcg cgcccctgac cgaggtcggg
     1681 gtcgacgcgc gggtcggcga ctacgtcctc gccgtcgacg gcgagcctct ggccgggacc
     1741 gacaacccct atcgcctcct gcagcacaag acccaccccg tcaccctcac gctcaacgcg
     1801 aagccgaccg tcgagggggc acggaaggtg acctaccggc cggtcgagaa cgaggcgagc
     1861 ctcctctacc tcgactgggt gctcggcaac gtggagaagg tctcgaagct ctcgaacggt
     1921 cgcgtcggct acctccacgt cccagacatg ggcgccccgg ggatctacga gttcctcaag
     1981 tggtactacc cgcagatccg aaaggaaggg ctcgtcgtcg acgtccgctc gaacggaggc
     2041 gggaacgtct cgcagtggat cctcgagagg ctcgactcga agctcctcgg gacccgcttc
     2101 ggcttctcga gcgacctccc gatgacgtat ccctacgccg ttttccacgg ccacctggcc
     2161 gcgctcatca gcgagacctc ggcgtcggac ggtgacatct tccccgcgcg cttccggaag
     2221 gcggggctcg gccccctcat cgggaagcgg acgtggggcg gcgtcgtcgg catctcgggg
     2281 cgcggaccgc tcatcgatgg cggccaggtc ttcgtcccgc agcaggcgac gaacgacgtc
     2341 gacggcgcct acatcatcga gggggaaggg gtcgcgcccg acatcgaggt cgagaacgac
     2401 ccggcctccg tcgccgccgg ccgcgacccg cagctcgagc gcggggtcgc ggaggtgctg
     2461 aaggcgatcg agaaggagcc gaggacgctt ccgaagcgtc cggcggaccc ggtcaagacg
     2521 aagtaggcgg ccgtgagggc cggcggcggg tcaacgcccg ccgccggccg gccgcgcgtc
     2581 cgcgatcacg aggaggccgt ccggcctcca gccgaggtcg aggccggcgg gaaagatccg
     2641 cgactcgcgg gcggaataga gcgggatcca ggggagctcc tcgtcgagga ggtcggagac
     2701 ctcgcggatc cgcgtcaacc gctcctctgc gcgggtggcg aaggtgaggc gagagaacgc
     2761 cgcgtcgacg gcggcgctcg agaagccggt cctgttcatc gagccgaggc cgcgcgaggc
     2821 gtcccgggtg tggaaggcgt tccgaagggc ctgccccgcg tcgcgcccga cgaaccagct
     2881 gtagaggtag agcgggcttc gtccctcgat ccgcgtcacg aagtcgtccg gacggtggac
     2941 ccggacgcga agacggattc ccgcctcact cgcctgggcg gcgagggcct ccccggcccg
     3001 gcgcccgttc gggccgacgt cgaggtcgag atcgaagccg tgcgggtggc cggcctcctc
     3061 gagcagccgc cgcgcggtcg cgaggtcgcg ccgaggcggc cggcgtcccg ggtcgaatcc
     3121 gaagctcccc ggcggaacga ggcggccgac cggcgtcccc cccgcgaccg tcccggccgc
     3181 gacgagcgcc ggcaggtcga tggcgaggcg gagggcccgc cggacgcgga ggtccgagag
     3241 cggtcccgcg ccggcggaga agccgatcgc gagataagtg agcccgccgg agtcggtggt
     3301 gacgacgcgg aggcgcggat cgccccgcgc tgcggcgacc atcgcgggcg ccgggtcggc
     3361 gacggcgatc gggacgtcgc gtcccatcag ccggagcccc tcggcggcgt tcgcgaagcg
     3421 tcggaagacg acctcgtcga ggtagggagc cgggcgggag gggcgctcgt ggcgggcgag
     3481 gacgatccgg tccggcgtcc gggcgacgac ccggtagggt ccgctgccga cgaagctctc
     3541 tccctccttt cgggcgatga agagcgcgga gacggaatgg aggaagtcct ccggggcgcg
     3601 aaacgtccgg aagcggaccg tccgcgcgtc ggccacctcg atcgcgcgga ggtctgcgag
     3661 ggaggcgacc gagccccgcg cggtccgcga ggcatgcgcg gcctggacca cgtcgcggct
     3721 cgtcacgggg ctcccgtcgt ggaaggaggc cccctccgcg atccggaaga gccaggtgtc
     3781 gggcgcgggg ttggtccagc tctgcgccag cgcggggcgg aacccggcct cgtgttccgc
     3841 gacgagcggc tcgtagatcg cggagaagac gcttcgcgag acgagccggg gatcggagta
     3901 cggatcgagg gattccggga ggaccgggac gaggacctcg acgcttcgcc gaaccggccg
     3961 ctcccggcag gcgccggacg gaagcagcgc ggcggcgacg acgagcagcg ccgggagccg
     4021 cacgccgatg cggcccgggc gcctgccgcg ccgccggtcg gggtcgtcgc ccgcggtcgt
     4081 cacctttcgg gcaggagctc cttcacgagg tgggccgccc cgtccacctc ctccatcatc
     4141 cagagcgcgt agcgcaggtc gacgttcacg ttccgggagc gctccggcct ccagccgaag
     4201 tccccggcga cgttctccca gacgcggtcg aagttgaggc cgacgacctc gcccttcccg
     4261 ttcatcgtgg ggctcccgga gttgccgccg gtcgtgtcgc cgtcggtcag gaagccgacc
     4321 gggacgctgc cgagcgccgg gtcggaccag cggccaaagc ggtgggcgct cgccgcctcg
     4381 aggaccttcc cggggacgac gaacggctcc tcggcccgcg ccttctcgag gacgccgagg
     4441 aggctggtgc gcggcgtgaa cgtcaccgcc tcgcgcggcg agtagccctt cacggtcgcg
     4501 aacgagacgc gaagcgtgga gttcgcgtcg gggtagatcg cctgcccctt cgagcgccgg
     4561 agcgcctcga gcgccgagag gtagcgcggc cgccagacga ggagcgcccc gttcgtctcc
     4621 ttccgcctct gccggatcgc ggcgaggtcg gcgtggagcg ccacggcgag cttcaggtag
     4681 gggtcggcgg aggcagagag ggaggcgaac gtcgcgtcga cgttcgcgag ccgcgccttc
     4741 tcgtccgaga gcgaggtcgc cgcgtccatc ccggcgagcc gcgcctcgat cgcggcgcgg
     4801 tcggcgtcgc ctgcgaacgc ctcgtcgaga gcccggatcg gagctccctt cgaggccgcc
     4861 cgggccttct cgacgaggta ggcgagcgcc ttccgcgtcg cctcgggcgc gagcgagagg
     4921 gtccggtcct tctcctgctg ccgggcccgc tcgaggtcgc gctcctggaa gccgggctcg
     4981 cgctcgaggt ccggcttccg ccgttcgtcg gcgcggcgga cggaggagag ggccgaggag
     5041 agatacgagg agagccgctc gatctcctcg aggaagaacc ggcgctcttg cccctcgcgg
     5101 tcgcgcgcga ggaccttctc gagctcggcc ggcgcggccg tccaatccgc cgggaggtcc
     5161 ttgcgggcgg cgaggaactc ctccagctcc ttcgcctccg cgcgggcgcg ggcggcaacg
     5221 ccgttgcggg cgagcccctc gatctgcccg cgcgcgttcg tctcgccgtt gccgtacccc
     5281 ttgacggcgc cggagacgcg cagcgcggtg tcggggtcct tcttcccttc cgtctcgagg
     5341 atcgcgatca ggtcggtgaa cgtcctcgag cggagcgggt agaaccactc ttcgaggttc
     5401 tcgacggcgg acggcgtcag gtagcgctgc gtccggccgg gatagccggc gatcatcacg
     5461 acgtccccct cggagacgcc cctcgtcgag acccggagcc agcgcttcgg ccggtagggg
     5521 acgttctcct tcgcgtgagc ggccggcgcg ccgtcggggg cggcccagac ccgaaggagt
     5581 gtgaagtccc cggcgtggcg cggccagcgg aagttgtcga cctcgccgcc gaagtcgccg
     5641 acgccccggg gcggggcgta gacgagccgg acgtcccgga actcggtctg ctccatgagg
     5701 cgataggtcc ggccgtcctc gaacgccgcc acggcgcagc gccggtcggg ctgcttctcg
     5761 cactcggcga cgatctcgct tcgggccttc tcgaccgccg cctcgcgggc ggcgtcgtcc
     5821 ttcgcccgcg ggaaggcgct cttcggcccg cggacccggt cggtcacgtc ggagaggcgg
     5881 gtcaggatcc tcacccgccc gacgccgccg cgcgccggca cctcctcggc gcggctccgg
     5941 gcaacgaagc cgtcgcggag gtagttcttc tccggggtcg aggcgagctg gatcgcgccg
     6001 aacgcgcagt ggtggttcgt caggaagagc ccctcgtccg agacgaacgc gcccgtgcag
     6061 ccgccgaggc cgaggacggc ggtcaggagc ccgcccccct ttccgtccca gaggtcggtc
     6121 gcgccgagct cgaggccccg cgcggcgagc tcggcccagc gggcctcccc gagggtggcg
     6181 agagcctccg gaggccactg cccttcgtcg gaggaggctg ttcccgggag ggcgaggagg
     6241 acgaggagcg gaaggaggag ggcggcggtg cgaaggcgcg gcgtcatgtc ttctccggca
     6301 ggatcgtagc ccgccgcggg acgccgcgcc tcgtccgttc gggctacttc gcctcttctg
     6361 cgaggaacgc cttcatcgcc tcccaggagc ggcggtcggc cctggcatcg taggcagccc
     6421 ccttcgacgg gtcgttcccc gccgcagggt tcgtgaagga gtggaccgcg ccggcgtagt
     6481 agaccatctg ccagtcgacc ctcgcgtcgg ccatctcctt ctggaacgcg gcgacctcgg
     6541 ccggcgggac gtacgggtcg gccgcgccgt ggaggaccag gacgcgcccc ttcacgttct
     6601 tcgcgtcggc cgggtccttc gtgccgaggc cgccgtggaa cgagacgacg cccgccaccg
     6661 gggcgccgct ccgggccagc tcgagggccg tcgtcccgcc aaagcagtag ccgatcgcga
     6721 agagccgcga cgcgtcggcg cggggatcct tcgcgagcgt gtcgagcgcg gcccgcgccc
     6781 gcgagcggag gagagggacg tctcccttga accggccggc gagccgcccc gcctccgcgg
     6841 cgtccttcgc gcggatcccc tttccgtaga tgtcggcggc gaacgccacg tagccgagcg
     6901 cggcgagctg ccgggccctt tccttcgcgt ggtcgcccag ccccatccac tcgtggacga
     6961 cgacgacgcc gggacgcttt ccctgtagcg cgtcgtccca ggccaggaac ccttcgcaga
     7021 gctggtcgcc gtcgaggtac tcgacggtcg cggacttcac ggcggcggcg gcggggagcg
     7081 ccagggcggc gatggcggcg agagacgcgc agacgacgaa cgtacgcatg acgagggcct
     7141 cctcggctcg tgatccagga cggagcggaa gatagccgat gggcgccgga ttcgagaagg
     7201 agtcgcgcgg cgaggatccg gagcagtccg ccgccgttcg tcgggccccc cggcatggac
     7261 ccgccggccg ggtggagcga cgtgctagca ttcccgcgtt cgaaatccaa attgaagctc
     7321 ggttctctgc gagggcaccg aaggtccagg cggcgatcca ctctcggctc gtgaaaggcg
     7381 gtccgtatgt ggggggtgaa gtccgggcac cggaagagcg gcgccgcctc gggcggccgc
     7441 cgtgcctgtg tgctcgatca cgcaatgaag gccggctgag aggccggccc ggcaggtcca
     7501 atccactttc ggccccaggc aggggccatt ttcgaaggga ggaaggaacg atgaagagac
     7561 tcgggctggt tttcgtcttg ctgatggtgg cggtcatcgc cgccccggcg gacgcccagg
     7621 agcagacggg cgtcctcacc ggccgcatca cggccgacgg gggcgaggcg ctccccggcg
     7681 tgtcggtcga gatcaagggg cccagcggga cgctcgtctc cgttacggac gcgcggggcg
     7741 actaccgctt cccccgcgtg ctccccggcg tctacacggt cacggcgcgc ctgcagggct
     7801 tccagaccta cgaggcgtcc cgcgtcccgg tgtccctggg ggaccgcgcg atcctgaacg
     7861 tcgcgctcaa gatcagctcg gtcatggaga cggtcgtcgt caccggcgag cgggtccaga
     7921 tcgcggtggg cgagaactcg accacggcct cgatggccgc cgagcagatc gcgctcctcc
     7981 cgaaggggcg tgacttcacc tcggtcgtcg cgcaggcccc cggcgccgcg aacgaggcgt
     8041 tcgcgggcgg catctcgatc gacggcgcct ccgggtcgga gaaccgcttc gtcatcgacg
     8101 ggatcgacac gacccgcccg aacgacggcc tctcgggcca gagcttcatc accgacttcg
     8161 tcgaggagat ccaggtcaag tcggccggct actcggccga gttcggcggc tctctcggcg
     8221 gcgtcatcaa cgccatcacg aagagcggca cgaacgagtt ccacgggtgg ctcggcggct
     8281 acttctcgag ctccacgctg aacggcgacc ggcgccccat cgcgtacgag tcgtggccgg
     8341 gcttctaccg gacgttcgac aaggacgacg agtcccgctt cgagccgggc ttcgccctcg
     8401 gcggcccgat catcaaggac tccctctggt tctacctcgg ctacagcccg acgctccgct
     8461 cgatcgagcg gacgccgtac cagaaggcga cgaccgccga gcaggacatc accgaccatt
     8521 tcttcgcggg gaacatcaag gggaacatcg gctccaagct cgtcttcaag gtctcggcga
     8581 acgtcacgcc ttccgagacg gacgggaacc tcccggccct cgacggctcg acgccggacg
     8641 acgccgacct cgacgtcgta tcgaagtacc cgacctcgag ctactccgcc tacgccgact
     8701 tcgtccccac gagcaacttc tacgtgagcg gccgcgtcgg cctgttcgag accgacaccg
     8761 agatcaccgg catcgacgcc cagaaccgga tgctcttccg gaacggcgtc atcccggtcc
     8821 cggagaccga cccgcgctac cgccccaccg gtttcgcgac gatcccggac gcctcgttcc
     8881 agagcgccga cgaggacaac tggaagagga cgtcggccgg catcgacgcg aacctcttcg
     8941 tcacggccct cggctcgcac tccttcaagg cgggcgtcca gtacgagaag atcgagaact
     9001 cggtctccct cggcgagaac ggcaactact tcgaggtccg ctggggcctt ccggaccggt
     9061 tcggcatggg cgtccagggg acctacggct ccatcggcgt ccgccgcttc cggaccgagg
     9121 gcgccgccga ggcgtcgaac gtcggcctct tcatccagga cacctggaac gtccacccga
     9181 acctgacgct gaacatcggc gtccgcaccg agcaggagcg gatcccgaac tacggcgcgc
     9241 aggtcgaccc gacccttccc aagaacgcga tcgagttcga cttcgaggac aagctcgccc
     9301 cgcgcctcgg cttctcctgg gacgtcacgg gcaagcagca gctgaaggtc tacggctcct
     9361 acggcaccta ctacgacatc acgaagctcg agatgccgcg cggctccttc ggcgccgacc
     9421 ggtggatcca gcacatgtac gagctgaaca cgctcgactg ggacacgttc ttcgcggcct
     9481 gcgcgcgcgt cgacaacacg ctcgggcaga acccctgccc cacgctcgtc ggccacaagg
     9541 cgcgtgacct gcgtgccccc ggcgacccgc acgacagcat cgaccccgac ctccagccga
     9601 tggagaacga ggagatccag ctcggcgtcg actaccagct gacgaagaac tcggtcgtca
     9661 gcgcccggta cgtcaacaag aagctcaaga acacgatcga ggacatcggc tacctcgtct
     9721 tcctgcccga cggctcctcc gaggagcagt acatcaccgg caacccgggc aagggcatcg
     9781 tctccggcga cccggccggc gcgatcccgc cgcaggccga ggcgatccgc gactacgagg
     9841 cgatcgagct gacgttcgcc cgccgcttcg ccgacaactt ctcggtccgc gccgcctacg
     9901 tctacagcaa gctctccggc aactactccg gcctcgcgag ctccgacgag ttcggccgga
     9961 ccgacccgaa cgtcgcccgc tacttcgacg gcctcgtcta cggctacgac tccgagggcc
    10021 gcatcgtcga cgacgtcctc aacaccgacc ggccgcacgc cttcgaggtc cagggcctct
    10081 accaggcctc ctggggaacg aacatcggcc tcagcttctc gtgggcctcg ggcagcccgg
    10141 tctcgaccga cgcttcctac aacggcgtga acttcttccc gaacggccgg aacgacatgg
    10201 gccgcctctc cggcctcacc cagaccgacc tcctcctcac ccaccccttc aagatcgggg
    10261 gctacgagct cgaggtctcc ctcaacgtcc tgaacctctt cgaccaggac accgtgaccc
    10321 ggatcgacaa cgtcaagtac ttcgacgaca tctgcgacgt gtcggccgag tgcgacggct
    10381 cgaacgactg gtacttctcg gatctcgtcc cgtacgacta cagcacctac atggacgcga
    10441 acggggcgtc gaagaacccg ctctacggca agcccctcac ctaccaggcc cccccggacg
    10501 gtccgcttcg gtctgaagtt cgtcttctga cccggccggt cttccggtcc acggacggcg
    10561 ggagcgcgcg agcgctcccg ccgtcttctc ttcttgaggt gtcggcgaac gaggccggaa
    10621 gcccggtcgg agcccggcac ggatctttca agggaatcac cggacagata tgtggacgca
    10681 tccggtcccg tccgccgcgg aggcggaaag tgagcgcgat cagcagctca ggcgcctgct
    10741 tcgaccttgc gttgcggccg ggaccgctat aatgccgcgc ccctggctcc gaaaacccgt
    10801 accgcgctcc ggtttcttgg ctccgggtcg gagcgtcccg ttccgggacg atcccccgca
    10861 aagtcccccg ggcggggaaa ggaggtccga ggagagagtc gaagtgaaga gacgacgcca
    10921 cgctgcgagg cgaccggtct tgccgacccg gtccgccgga cgggcgtgaa cccgatctcg
    10981 tgtgccaagc ctagaatcaa aggcccaacc tcaatttcgg aaataccgga gggaataagg
    11041 acatgaatgg aaagcgactc tggctcctcg tcctcctcgt accgttcctg ctggcggtgc
    11101 aggcggacgc ccaaggtctc ccgacgggca ccctgaccgg gcgcgtcgcc gaggccgagg
    11161 ggctcgcgct gcccggcgtg acggtcaccg cgaagtctcc cgcgcttcag ggcacgcgaa
    11221 ccgccgtcac gaacgtgaac ggcgacttcg tcatcccgaa ccttcccccg ggcgaatacg
    11281 tcgtcgcctt cgtgatgtcg ggcttccaga acgtgacgag gaacgtcaag gtctcgtccg
    11341 gccagcaggt cccgctgaac acgaagctgt cgatcgccgc cgtcgccgcc gaggcgatgg
    11401 tcgtggccca gagcgagacc gtctcccaga cggcgcaggc ctcgaccacc tactcgaccg
    11461 agctgacgaa caagctcccg gtcgcgcggc agatcctctc ctcggtcgtc ctgagcgcgg
    11521 gcgtcaacac gaacggcccc ggcggcaaca cgacgatctc gggcgcgcag tcgttcgaca
    11581 acgtcatcac cgtcaacggc gtgaacatcc aggacaacat ccgcggcacg ccgacgaaca
    11641 cgctcgtcat cgaggacgcc atccaggaga cgacgacgat gacgtcgggc gtctcggcgg
    11701 agtacgggcg cttcacgggc ggcgtcatca acgccgtcac gaagcagggc ggaaacgcct
    11761 tcagcggctc cttccgcgtc acgctgaaca acaacgactg gcagtcccag ggtccgatcc
    11821 ggcagaactt cgtcgacaag gtcgtcccga cctacgaggc gacgttcggc ggcccgatcc
    11881 tgaaggaccg cctctggttc ttcggcgccg gccgctacca gaagaacgac tactcgggcg
    11941 ccctgtcggc ccccctcacc ggctccttcc cgcgctccga caaggacacg cggtacgagg
    12001 ggaagctcac cttcaccccc ttccagaacc acacgatcac gggctcctac acgaagggtg
    12061 aggtcgagca gtcgaactac tacttcacct cgttcccgat cctcgagacg aacggcgtca
    12121 cctacgaccg ctcgctcccg acggacctcc tctcgatcaa ctacaacggc gtcctctcct
    12181 cgaacttctt c
//
LOCUS       DAOONL010000095        12148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5808_length_12148_cov_22.775821, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000095 DAOONL010000000
VERSION     DAOONL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_12148_cov_22.775821"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLP50_12750"
     CDS             complement(<1..301)
                     /locus_tag="PLP50_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPA52458.1"
                     /translation="MADRRRPVERARRPETSGARESADEVLRAYEATLEGWIRALDLR
                     DRETEGHSRRVVGLAEKLAEELGVCGEKLVHIRRGALLHDVGKMALPDSVLLKPGE"
     gene            549..1232
                     /locus_tag="PLP50_12755"
     CDS             549..1232
                     /locus_tag="PLP50_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPA52459.1"
                     /translation="MTNSLPPQPEAAPRGGRLLSAAELLFGAALVLGHNVWRVLPNEV
                     PLLFVLGLASYRLRSGGFAAIGFRRPPSWMRLLAIALGAAALRIALGIVVEPLAESIW
                     PPIVAPSGAEAIASDPWTALRWLGLVWTWAAFGEEIGYRGWLLGRAADALGRSPAATA
                     FAVAASSVLFGFGHFYKGPAGVLDSGIAGLVLAGAFLLARRNLWAPILAHGFIDTFGI
                     AALYFGWAD"
     gene            1333..1797
                     /locus_tag="PLP50_12760"
     CDS             1333..1797
                     /locus_tag="PLP50_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPA52460.1"
                     /translation="MLDSLKRFFRERVLDAPTAEAGDPGRRLRLAAATLLVEVMRADP
                     DVKDEERTVVRTALQGTFGLSLAETEELVRLAEAEADAATSLYEFTSLVAEGFSREER
                     KRIVELMWLVAFADAEKHPLEEHLIRRLAGLLKVPHPDFIDAKIRARKESRT"
     gene            complement(1831..5403)
                     /locus_tag="PLP50_12765"
     CDS             complement(1831..5403)
                     /locus_tag="PLP50_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52461.1"
                     /translation="MKAKAPSAAAIAAFEWRTATRRLPFAAAALLMGLMGFALSATAF
                     GPPELLLNGAWSVTSSVGFLTLVSVFAAILLTAPVLLRDEEHRMAEIVYTTAVTKRGY
                     LLGRFAGALLATATAVLVGVAGLAAGTFLVPHDPARLAPFAPLPYLWAFAVLALPGVL
                     LVSACLFAVAALTRSTPATYVAGVLLYLLYFVAALLADSPLLAGSGPQSAGAASLAAL
                     LDPFGLSAFLSDTRFWTVAERNERLVPLAGTFLGSRLVGLGAAGLALTVAYRRFAFRL
                     APGAGRVAADAGPERSDGPRAPSPGAVTTTPGLAAFGSTLRVELRALFRSAPLAALFA
                     LWIGYLAIETVQSFRSNELGTAVIPTAGLLVADVATALNVLGILFLVFFAAETAWRER
                     LVRVSEVVDATPASSALVLLGKLGALSAVLVALAASGLATALAFQAVVGRFELAPLTW
                     AALLPVAVLPLVLLALLVLLVQALVPNRHVGLVLSLAAIAFLHKGALGGPDHPLLRYA
                     YAPRLTWSDLSGLSPGAESLGWFAAFWSAVAALLGVVTFGLWPRGTDPRLAPRLAALP
                     RRWGRGGRLGAIACATLALAVGGFAFHGTNVANRYETEADLLAWKAAYERSYRPIEAR
                     AQPMPAHLDAAFDLEPEARRLRVRGRLRLENRTPAAIDSVWVAARRDVRVASMTLAGA
                     VPSDVDERFATRRFALASPMAPGAALDLAFDLLLERRSPSAADDADLVPNGTYVHGLA
                     ALPTIGYRRSWEISDPAARRRAGLPERAVDDDPDAHLPESPSRMTFDVRVTTPANQHA
                     VAPGALVSSTVAGDRRTSRFRAARPVSAYFAFASARYHVERVRHVGVDVEVYVHPGHR
                     RNVARVLEAATRTLDLCVARYGPYPFPQLRIAEVPDASLRAGGFALPGVVFLAERRGF
                     LIDAEAPGRVDLLAKRVAHEVSHQWWGHQLDPASGPGATALVESLARHTELRVLKSLR
                     GAEALRPVLDHELDRYLTGRTAGGEVPLVEVGRQPWLYYAKGSLAMAAISELAGEEAV
                     DGALGGLLADAETTGRAPTARDLVARLLAATPAADRPLVEEWWTRSVLHDLRITEAIA
                     TRLPDGRYRVDVAVDGRTVEATEGAEKERPFGGDVELVVRAARSDGGDDEAPPLHSAR
                     HRVRGPARLSVLVDGRPGEVLLDPRRLLLDRDRADDARDVETRR"
     gene            complement(5400..6275)
                     /locus_tag="PLP50_12770"
     CDS             complement(5400..6275)
                     /locus_tag="PLP50_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52462.1"
                     /translation="MLSIADVSKTYPNGVKALRGVSLEVGTGLFGLLGPNGAGKSTLM
                     RTLATLQEPDSGRISLDGADVLADPQGLRGRLGYHPQEFGVYPGISAEALLDHLALLK
                     GIADRRERAAQVAALLQRTNLHEHRRRAVEGFSGGMRRRFGIAQALLGDPQLVIVDEP
                     SAGLDPEERHRLHDLLVEAAESRIVLLSTHIVEDVRQLCARMAVLSEGRVLLQGKPGP
                     LVESLAGRVFRKAVSREELAACRSSLTVLSTQLRAGRTHVQVLADARPDAGFEPVAPD
                     LEDVYFAALRSGEAA"
     gene            complement(6346..10485)
                     /locus_tag="PLP50_12775"
     CDS             complement(6346..10485)
                     /locus_tag="PLP50_12775"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA52463.1"
                     /translation="MTLSRTAVLLAGALLATPPAAASLRGKVLLDGRPLAGATVAAIP
                     QEESLEVARREARGEPEPAALATVATLATGEFDLRVAAPEGRDVFFRVRVSATGAVPV
                     ELPETRDATEEEDLGDLALRRAEPLAGRVRGPDGAPVAGALVRLVARGRADGPVTGTA
                     QPQEATTGADGQFRFATAAAERNELRVRAAGFAPARVRNAKPGALPREISLEPGHSLS
                     GIVRKKDGRPAAKALVRWEGNDAESRWVETGEDGSFRLADLPKRRGTVVADGGEHGFA
                     EAGGATPGSAEPLVLSLAPPAILEGRTLDASTLKPVPRVRLEVATDSGRFTARTGHDG
                     RYRVRGLRPGSATVRAEEPRHVPWTRHSVPIEKGASRTLDVLLTRGATLSGRVVDEDG
                     RPVAGAGVSLLSSSESSLDAAVRLRGEASRLRTRPDGSFTATRLPPGENQRLSVQHPE
                     FERGTVGGISLVAGGTRSGVVVTIRRGVVLTGRVSDPDGNPIAGAEVSAGRSFSVRSS
                     SGGSLTRVRVEMMEAPRTARSGGDGAFELRGLAPGGVSVTVKAPGRATETVDPVLLER
                     GARPDPVEIVLEPGATISGRIVRRTGEGASGYFVLAQNPRKPSGGRGSTSATPEADGT
                     FRIEGLRAGETYDLQLFGTSAGFGPGPRKAGILAPADGVEWVVEGTGRIEGSAVDAKT
                     GQPLGAFEVSYQADRPGMGGVFRVGRGGSRSTGGAGEAQPVESPDGRFALEEVPSGKW
                     QVVVTAKGYQVGRAAGVVVEDATTTDGVEVRVAPGSVLRGIVKDARSRRGVPDARVRV
                     RGETGDGGSSQGPGGLFTDVEGRFEAEGLAPGKVTVTAEHADYTARTESAVLAEGGSS
                     VEIALSRGSGLGGVVLSETRQPVAGASVSLEGGAARGGGFGGGDETTTDGAGRFRFDH
                     LSPARYTLRASVPGQSSEPAEAVVVEGEAVPDVTLVLSGGATLRGSVSGLPETLRGAV
                     NVSANGPRDYWATVRTGPDGRFELSGVPAGQVSVRASAGDLASGTMRSANASVSIAEG
                     QTEAEVEIVFEGDGALSGRVTRGGKPVADARIVAGAARTGRSASGRTDETGAYRIEGI
                     TPGEYEVWVQADAYGRAGNVSRTVSIDGEARLDVELPVASLFGIVVDASTKQPLAEVR
                     VAASLEGGAEGRPGAATSDSNGRFFVEELEEASYTLSLRRSGYQDAKSSARASEGGGD
                     AGTIEMTRGDGLEVRVRDGIYGIPLRSALVRVKDGRGDVGASTWLTLDGDGKGEIAFL
                     RPGTYTIVVAGSHYAPRTFENVGVPGPALQVTLTPGGMLELKYGPVAIAKGFATVLDA
                     LGRPYAFRAYGPEGRITLTAAGYATIEGLAPGSYTLSVEGMEPKAFSVVEGGKALVEL
                     P"
     gene            complement(10609..12024)
                     /locus_tag="PLP50_12780"
     CDS             complement(10609..12024)
                     /locus_tag="PLP50_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPA52464.1"
                     /translation="MTVPREGVVASLRALFGENAVVVEPRELERYEKGWRYGRGRALA
                     AVRPSSTEEVSRLLRFASSIGLRVVAQGANTGLVGASTPDGSGEMLVLSLERLARTLE
                     VDPVERTVTADGGVLLSRIDAALEEHGLLFPIDLGADPSIGGMVATNTGGTRLLRYGD
                     VRQNLLGLEAVLADGTVLDLTRPLRKNNTGLDVRQLFVGTSGVFGVVTKVVMRVIPRP
                     AQRVTAFVGLSSGAAVLGLLAHLESRLGELLTAFEAIGAAALTPVFRHQPRLRDPFAG
                     RPPAYAVLVELSSTLGAERLALDGLLESALAERLEAEDGGITDVFPGKPSELWAIRHG
                     VSESLRQEGETLAFDVSVPRSSLADFVEAARATVVGGWPFVRFCDYGHWGDGGVHVNL
                     IWDPVAAPRPAAELKAELQPRLYDLAVVRFGGSYSAEHGVGPHNQAFYDLYTPDLVKE
                     VCRLLKARLDPTGLLGTVRLG"
BASE COUNT         1632 a         4542 c         4527 g         1447 t
ORIGIN      
        1 gctcgcccgg cttcaggagg acggagtcgg gaagcgccat cttcccgacg tcgtgcagga
       61 gggcgccgcg gcggatgtgg accagcttct cgccgcagac gccgagctcc tccgcgagct
      121 tctccgcgag cccgacgaca cggcgactgt gcccctcggt ctcccgatcg cggaggtcga
      181 gcgcgcggat ccacccttcg agcgtcgcct cgtaggcgcg gagcacctcg tcggccgact
      241 cccgtgcccc cgaggtctcc gggcgccgtg cccgctcgac cggcctccga cgatccgcca
      301 cccgtcctcc tgcccggcac cgtagcgaac gcgcgcgagc cgggtcaagg aggcgctgtc
      361 cgagcccccc gtcccgcttg ccgaagagcg ggcgccccgc cacactgagg catggggctg
      421 tcggcggagc tcccagcagc gcggcgtgcc ttcccctttg cctgcatgac cgtctgcctc
      481 gcgctcctct ccgcatgcgg cccgcggccc cctgaacccg tacccgccgc cggacgttac
      541 gagacacgat gaccaactcc cttcctcccc agccggaagc cgccccgcgg ggcggacgcc
      601 tcctctccgc ggcggagctc ctcttcggcg ccgccctcgt cctcgggcac aacgtctgga
      661 gagtcctgcc gaacgaggtg ccgctccttt tcgtcctcgg cctcgcctcg taccgactcc
      721 gcagcggcgg cttcgcggcg atcgggttcc ggaggccgcc gtcctggatg cgactcctgg
      781 cgatcgccct cggcgcggcc gccctccgga tcgcgctggg gatcgtcgtc gagccgctcg
      841 cggagagcat ctggccgccg atcgtcgccc cttccggcgc cgaggcgatc gcctcggacc
      901 cgtggacggc cctgcgctgg ctcggtctcg tctggacctg ggccgcgttc ggcgaggaga
      961 tcgggtaccg cggctggctc ctcggacgcg ccgctgacgc gctcggacga tcccccgccg
     1021 cgacggcgtt cgccgtggcc gcctcctccg tcctcttcgg cttcggccac ttctacaagg
     1081 gacccgcggg cgtcctcgac tcggggatcg ccggcctcgt cctcgccggc gcgttcctcc
     1141 tcgcccgccg gaacctctgg gccccgatcc tcgcgcacgg gttcatcgac actttcggaa
     1201 tcgccgctct ctacttcggc tgggccgact gagcgggcga gacggcgccg cgaggcgctc
     1261 gaggacgggg atctcggcgg cgcgaacacg gccggggtgg acggggccgg cgagggacga
     1321 gagactccgg gcatgctcga ctcgctcaag cggttcttcc gggaacgggt cctcgacgcg
     1381 ccgaccgcgg aggcgggcga tcccggccgg cgcctccgcc tcgcggccgc gacgctcctc
     1441 gtcgaggtga tgcgcgccga ccccgacgtg aaggacgagg agcggaccgt cgtccgcacg
     1501 gcgctccagg ggacgttcgg cctgtcgctc gccgagaccg aggagctcgt ccgcctcgcc
     1561 gaggccgagg cggacgccgc cacctcgctc tacgagttca cgagcctcgt cgccgaaggc
     1621 ttctcccggg aggagcgcaa gcggatcgtc gagctgatgt ggctcgttgc gttcgccgac
     1681 gcggagaagc accccctcga ggagcacctg atccggcgcc tcgcggggct cctcaaggtc
     1741 ccccaccccg acttcatcga cgcgaagatc cgggcccgga aggaaagccg cacgtagcgg
     1801 ccctcccggc gggaccgggc ctgcggcccc tcagcgcctc gtctcgacgt cgcgggcgtc
     1861 gtcggccctg tcgcggtcga ggaggaggcg gcgcggatcg aggaggacct cccccggccg
     1921 cccgtcgacg aggaccgaga ggcgggccgg cccgcggacc ctgtggcgcg cggaatggag
     1981 cggaggcgcc tcgtcgtcgc cgccgtcgct ccgggcggcg cggacgacga gctccacgtc
     2041 gccgccgaac ggcctctcct tctccgcgcc ctccgtcgcc tcgaccgtcc ggccgtcgac
     2101 ggcgacgtcg acgcggtacc gtccgtccgg cagccgcgtc gcgatcgcct ccgtgatccg
     2161 gaggtcgtga aggacgctgc gggtccacca ctcctcgacg agcggacggt ccgcggcggg
     2221 ggtcgcggcg aggaggcgcg cgacgaggtc ccgcgcggtc ggggcgcggc ccgtcgtctc
     2281 cgcgtccgcg agcagcccgc cgagggcccc gtcgacggcc tcctcgcccg cgagctcgga
     2341 gatcgcggcc atcgcgagcg accccttcgc gtagtagagc cacggctggc ggccgacctc
     2401 gacgagcggc acctcgccgc ccgccgtccg gccggtgagg tatcggtcca gctcgtggtc
     2461 gaggacgggg cggagcgcct ccgccccgcg gagcgacttc aggacgcgca gctcggtatg
     2521 tcgggcgagc gactcgacga gcgccgttgc gcccgggccg ctggccgggt cgagctggtg
     2581 cccccaccac tggtgcgaga cctcgtgcgc gaccctcttc gcgaggaggt cgacgcgtcc
     2641 gggcgcctcc gcgtcgatca ggaagccgcg ccgctcggcg aggaagacga cgcccgggag
     2701 agcgaagcct cccgcacgga ggctcgcgtc gggcacctcc gcgatccgga gctgcgggaa
     2761 ggggtagggc ccgtaccgcg cgacgcagag gtcgagcgtc cgcgtcgccg cctcgaggac
     2821 ccgcgccacg ttccgtcgat gccccgggtg gacgtagacc tccacgtcga cgccaacgtg
     2881 ccggacgcgc tccacgtggt agcgggccga cgcgaacgcg aagtacgcgc tcacggggcg
     2941 cgccgcccgg aagcgcgagg tccgccggtc gccggcgacc gtcgaggaaa cgagcgcccc
     3001 cggcgcgacc gcgtgctgat tcgccggcgt cgtgacgcgg acgtcgaacg tcatccggct
     3061 cgggctctcc gggaggtgcg cgtccgggtc gtcgtcgacg gcccgctccg ggaggcccgc
     3121 gcgccgccgc gcggccgggt ccgagatctc ccagctccgc cggtagccga tcgtcgggag
     3181 cgcggcgagg ccgtggacgt acgtgccgtt cggcacgagg tcggcgtcgt cagcggcgga
     3241 gggggagcgg cgctcgagga ggaggtcgaa cgcgaggtcg agggcggcgc cgggcgccat
     3301 cggggaggcg agggcgaagc ggcgcgtcgc gaagcgctcg tccacgtccg acgggaccgc
     3361 gccggcgagg gtcatcgacg cgacccggac gtcgcgccgg gcggcgaccc agaccgagtc
     3421 gatcgcggcc ggcgtccggt tctcgagccg caggcgtccc cggacccgga ggcggcgcgc
     3481 ctccggctcg aggtcgaacg cggcgtcgag gtgcgcgggc atgggctgcg cgcgggcttc
     3541 gatcgggcgg tagctccgct cgtaggccgc cttccaggcg aggaggtcgg cctcggtctc
     3601 gtagcggttc gccacgttcg tcccgtggaa cgcgaagccg ccgacggcaa gagcaagcgt
     3661 ggcgcaggcg atcgcgccga gccttccgcc ccggccccac cgccgcggca gggcggcgag
     3721 tcgcggcgcg aggcgcgggt cggtcccccg cggccagaga ccgaaggtca cgacgccgag
     3781 gagcgcggcg acggcgctcc agaaggccgc gaaccagccg agcgactccg ccccgggaga
     3841 gagcccggag aggtcggacc aggtcaggcg cggcgcgtag gcatatcgca ggagcgggtg
     3901 gtcggggccg ccgagggcgc ccttgtggag gaacgcgatc gcggcgagcg agaggacgag
     3961 gccgacgtgc cggttcggga cgagcgcctg gacgaggagg acgaggagcg cgaggaggac
     4021 gagcggcagg accgcgacgg ggaggagcgc ggcccacgtg aggggcgcga gctcgaaccg
     4081 gccgacgacg gcctggaagg cgagcgccgt cgcgaggccc gatgcggcga gggcgacgag
     4141 gacggccgag agcgcgccga gcttcccgag gaggacgagc gcgctcgagg ccggggtcgc
     4201 gtcgacgacc tccgagacgc gcacgaggcg ctcgcgccac gccgtctcgg cggcgaagaa
     4261 gacgaggaag aggatcccga ggacgttcag ggcggtcgcg acgtcggcga cgaggagccc
     4321 cgccgtcggg atgacggccg tcccgagctc gttcgaccgg aagctctgga ccgtctcgat
     4381 cgcgaggtag ccgatccaga gcgcgaagag ggccgcgagc ggcgcgctcc ggaagagcgc
     4441 gcggagctcg acgcggaggg tcgagccgaa ggccgcgagg ccgggcgtcg tcgtcacggc
     4501 gccgggcgac ggcgcccgcg gtccgtccga ccgttccggc ccggcgtccg ccgcgacgcg
     4561 gcccgcgccc ggcgccagac ggaacgcgaa ccggcggtac gcgacggtga gcgcgagccc
     4621 tgcggcgccg agtccgacga ggcggcttcc gaggaacgtc cccgcgagcg ggacgagcct
     4681 ctcgttccgc tcggcgaccg tccagaagcg cgtatcggaa aggaaggcgg agaggccgaa
     4741 cgggtcgagg agcgcggcga gcgacgccgc tcccgcgctt tgcggcccgc tccccgcgag
     4801 gagcggcgaa tcggcgagca gggcggccac gaagtagagg aggtagagga ggacgcccgc
     4861 gacgtacgtc gccggcgtgc tccgggtgag cgccgcgacg gcgaagaggc aggccgagac
     4921 gaggaggacc ccggggaggg cgaggacggc gaaggcccag aggtacggga gcggggcgaa
     4981 cggggcgagt cgcgcgggat cgtgcgggac gaggaacgtc ccggcggcga gaccggcgac
     5041 gccgacgagg acggccgtcg ccgtggcgag gagcgcgccc gcgaaacgcc cgaggaggta
     5101 gccgcgcttc gtgaccgccg tcgtgtagac gatctcggcc atccggtgct cctcgtcgcg
     5161 gagcagcacc ggcgccgtca ggaggatcgc ggcgaagacc gagacgaggg tcaggaatcc
     5221 gacggagctc gtgacggacc aggccccgtt cagcaggagc tccggcgggc cgaaggccgt
     5281 cgccgagagg gcgaagccca tcaggcccat caggagcgcc gcagcggcga acgggagacg
     5341 cctcgtcgcg gtgcgccatt cgaaggcggc gatcgcggcg gccgacggcg ccttcgcctt
     5401 cacgcggcct cgcccgaccg gagcgccgcg aagtagacgt cttcgaggtc gggcgccacc
     5461 ggctcgaacc cggcgtcggg ccgggcgtcg gcgaggacct gcacgtgcgt cctccccgcg
     5521 cggagctgcg tcgagaggac cgtcagggac gagcggcagg ccgcgagctc ctcgcgcgac
     5581 acggccttcc ggaagacgcg tccggcgagc gactcgacga gagggccggg cttgccctgg
     5641 aggaggacgc gaccttcgga gaggaccgcc atccgcgcgc agagctgccg gacgtcctcc
     5701 acgatgtgcg tcgagaggag gacgatccgg ctctcggccg cctcgacgag gaggtcgtgg
     5761 agccggtgcc gctcttcggg gtcgagcccc gccgagggct cgtcgacgat gacgagctgc
     5821 gggtcgccga ggagcgcctg cgcgattccg aagcggcggc gcatcccgcc cgagaacccc
     5881 tcgaccgcgc gccgccggtg ctcgtggagg ttcgtcctct ggaggagcgc ggcgacctgg
     5941 gccgccctct ccctccgatc cgcgatcccc ttcaggagcg cgaggtgatc gaggagcgcc
     6001 tcggccgaga tcccggggta gacaccgaac tcctgcgggt ggtagccgag ccggcctcgc
     6061 agcccctgcg ggtcggcgag gacgtcggcg ccgtcgaggg agatccgccc ggagtcgggc
     6121 tcctggagcg tcgcgagggt ccgcatgagc gtcgacttcc cggcgccgtt cggcccgagg
     6181 aggccgaaga gcccggtgcc gacctcgagg ctcacgcccc ggagcgcctt caccccgttc
     6241 ggatacgtct tcgacacgtc ggcgatcgag agcacgagac acctccgcca cctgcacggc
     6301 agaggaagga acgaagacca cgggggaccg gttcccggtt ctcccctacg gcagctcgac
     6361 gagcgccttc ccgccttcga ccaccgagaa ggccttcggc tccatcccct cgaccgagag
     6421 cgtgtagctg cccggggcga ggccctcgat cgtggcgtag cccgcggccg tgagggtgat
     6481 gcgcccttcc ggcccgtaag cccggaaggc gtacggccga ccgagcgcgt cgaggaccgt
     6541 cgcgaagccc ttcgcgatcg cgaccggacc gtacttcagc tccagcatcc cgcccggcgt
     6601 gagcgtcacc tggagcgccg gccccgggac gccgacgttc tcgaacgttc ggggcgcgta
     6661 gtgcgagccc gcgacgacga tcgtgtaggt ccccggccgg aggaaggcga tctccccctt
     6721 cccgtcgccg tcgagcgtga gccacgtcga ggcgccgacg tcgccgcggc cgtccttcac
     6781 cctcacgagc gcgctccgga gcgggatgcc gtagatcccg tcgcggacgc ggacctcgag
     6841 cccgtcgccg cgcgtcatct cgatcgtccc ggcgtcgccg cccccctcgg aggcgcgcgc
     6901 gctgctcttc gcgtcctggt agccgctcct tctgaggctc agcgtgtagc tcgcctcctc
     6961 gagctcctcg acgaagaacc tcccgttcga gtcgctcgtc gccgcaccgg gacggccttc
     7021 cgcacctccc tcgagggagg cggcgacccg gacctcggcg agcggctgct tcgtcgacgc
     7081 gtcgacgacg atgccgaaga ggctcgcgac cgggagctcg acgtcgaggc gcgcctcgcc
     7141 gtcgatgctc accgtgcgcg agacgttccc ggcacggccg tacgcatcgg cctggaccca
     7201 gacctcgtac tcgcccggag tgatcccctc gatccggtac gcgccggtct cgtcggtccg
     7261 gcccgaagcg ctccgtcccg tccgcgccgc gccggcgacg atccgggcgt cggccacggg
     7321 ctttccgccg cgcgtcacgc gccccgagag ggcgccgtcc ccttcgaaga cgatctccac
     7381 ctcggcctcg gtctgccctt ccgcgatgct cacggaggca ttggccgacc gcatcgtccc
     7441 ggacgcgagg tcgcccgcgg aggcgcggac cgagacctgt ccggccggga cgccggagag
     7501 ctcgaaccgc ccgtcgggcc cggtccgcac cgtggcccag tagtcgcgcg gcccgttcgc
     7561 ggagacgttg acggccccgc ggagcgtctc cggcagcccc gaaaccgagc cgcggagcgt
     7621 ggcgccgccc gagaggacga gcgtcacgtc gggcacggcc tccccctcga cgacgaccgc
     7681 ctcggccggc tcgctcgact gacccggcac gctcgcgcgg agcgtgtacc gggccggaga
     7741 gaggtggtcg aaccggaacc gtcccgcacc gtcggtcgtg gtctcgtcgc ctccaccgaa
     7801 cccgcctccc cgtgcggcgc ccccctcgag cgagaccgag gcccccgcca ccggctggcg
     7861 cgtctcggag aggacgacgc cgccgagccc gcttccgcgc gagagggcaa tctcgacgct
     7921 gctcccccct tccgcgagga cggcgctctc ggtccgcgcc gtgtaatcgg cgtgctcggc
     7981 cgtgaccgtc accttcccgg gggcgaggcc ctccgcctcg aaccgaccct ccacgtcggt
     8041 gaagagccca cccggtccct gcgacgagcc cccgtccccc gtctcccccc ggacccggac
     8101 ccgggcgtcg ggaacgcccc ggcgggagcg ggcgtccttc acgatgcccc ggaggacgct
     8161 gccgggcgcc acgcgcacct ccacgccgtc ggtcgtcgtc gcatcctcga cgacgacgcc
     8221 ggcggcccgg ccgacctggt accccttcgc cgtcacgacg acctgccact tcccggaggg
     8281 cacctcctcg agcgcgaagc gcccgtccgg cgactcgacg ggctgcgcct cgccggcgcc
     8341 gcccgtggac cggctcccgc cccggccgac gcggaagacg ccccccatcc ccggcctgtc
     8401 ggcctggtac gagacctcga acgcgccgag cggctgcccg gtcttcgcgt cgacggccga
     8461 gccttcgatc cggccggtcc cctcgacgac ccactccacg ccgtcggcgg gcgcgaggat
     8521 gccggccttg cgaggccccg gcccgaatcc ggcgctggtt ccgaagagct ggaggtcgta
     8581 cgtctccccc gcgcgaagcc cctcgatccg gaaggtgccg tcggcctccg gcgtcgccga
     8641 cgtgctcccc cggccgcctg aaggcttccg cgggttctgc gcgaggacga agtagcccga
     8701 ggcaccttca cccgtccggc ggacgatgcg accggaaatc gtcgcccccg gctcgaggac
     8761 gatctcgacc ggatccggcc gggcgccgcg ctcgaggaga accgggtcga cggtctccgt
     8821 cgcccgcccc ggagccttca cggtgacgga aacgccgccc ggggcgaggc cgcgcagctc
     8881 gaaggccccg tcgcctccgc tccgggccgt gcgcggcgcc tccatcatct cgacccgcac
     8941 tcgcgtcagc gatccgccgc tggaggaacg aacggagaac gaccggcccg ccgagacctc
     9001 ggctccggcg atcgggttcc cgtccgggtc ggagacgcgc cccgtgagga cgacgccgcg
     9061 gcggatcgtg acgacgacgc cgctgcgggt ccctccggcg acgagggaga tcccgccgac
     9121 ggtcccccgc tcgaactcgg gatgctggac gctgaggcgc tggttctctc cgggcgggag
     9181 gcgggtcgcc gtgaaggagc cgtcgggccg cgtgcggagc cggctcgctt ctccccgaag
     9241 ccggaccgcg gcgtcgagcg agctctcgga cgaggagagg agcgagacgc cggctccggc
     9301 caccgggcgg ccgtcctcgt cgacgacccg ccccgagagc gtcgcgccgc gcgtcaggag
     9361 gacgtcgagc gtgcgcgacg cccctttctc gatcggcacc gaatggcgcg tccacgggac
     9421 gtgccgcggc tcctcggccc gcaccgtcgc gcttccgggg cgaagaccgc gaacgcggta
     9481 gcgcccgtcg tgccccgtgc gcgccgtgaa gcgtccggag tccgtggcga cctcgagccg
     9541 gacgcgcggg accggcttca gcgtcgaagc gtcgagcgtc cgcccctcga ggatcgccgg
     9601 gggcgcgagg gagaggacga gcggctccgc cgagcccggc gtcgctccgc ccgcttcggc
     9661 gaagccgtgc tcgcctccgt ccgcgacgac ggtgccgcgt cgcttcggaa ggtcggcgag
     9721 gcggaagctt ccgtcctctc ccgtctcgac ccagcgcgac tcggcgtcgt tcccctccca
     9781 gcggacgagg gccttcgccg cggggcggcc gtccttcttc cgcacgatcc ccgagaggga
     9841 atggcccggc tccagcgaga tctcgcgtgg cagcgctccc ggcttcgcgt tgcggacgcg
     9901 agccggcgcg aagccagcgg cccgcacccg aagctcgttc cgctccgccg cggccgtggc
     9961 gaagcggaac tgtccgtcgg caccggtcgt cgcctcctgg ggctgcgccg tcccggtcac
    10021 cgggccgtcc gcgcggccgc gcgcgacgag ccggacgagg gctccggcga cgggcgcgcc
    10081 gtcggggccg cggacgcggc ccgcgagcgg ctccgccctg cggagggcga ggtcgccgag
    10141 atcctcctcc tccgtcgcgt cgcgcgtctc cggcagctcc accggtacgg cgccggtcgc
    10201 cgacacccgg acccggaaga aaacgtcgcg tccttcgggc gcggcgaccc tcaggtcgaa
    10261 ctcccccgtc gccaaggtgg cgaccgtcgc gagcgccgcg ggctccggct cgccgcgagc
    10321 ctcgcggcgc gccacttcga gcgactcttc ctgcgggatc gcggcgaccg tcgcgccggc
    10381 gagagggcga ccgtcgagga ggacctttcc gcggagagaa gcggcggcag gcggcgtcgc
    10441 gagcagggcg cccgcgagga ggacggcggt tcggctgagg gtcaagaggc ctccgtgcgt
    10501 tcaccggaac cgccaggcgc ggccccggga aacgggtgcg tgttaccgga agtgtacgga
    10561 agcgggcgat cgcccgtcgc ggaccgaagg gcggcgacga acccgccctc agcccagccg
    10621 gaccgtcccg aggaggcccg tcgggtcgag ccgcgccttg aggaggcggc agacctcctt
    10681 cacgaggtcg ggcgtgtaga ggtcgtagaa ggcctggttg tgcggaccca cgccgtgctc
    10741 ggcgctgtag ctgccgccga agcggacgac ggcgaggtcg tagagccgcg gctgcagctc
    10801 ggccttcagc tccgccgccg gccgcggcgc ggccacgggg tcccagatca ggttcacgtg
    10861 aacgccgccg tcgccccagt gtccgtagtc gcagaagcgg acgaagggcc agccgcccac
    10921 gaccgtcgcg cgcgccgcct cgacgaaatc cgccagcgag gagcgcggga cggagacgtc
    10981 gaaggcgagc gtttcgccct cctgccggag gctctcggag acgccgtggc ggatcgccca
    11041 gagctccgac ggcttcccgg ggaagacgtc cgtgatcccg ccgtcctcgg cctcgaggcg
    11101 ttcggcgagc gccgactcga ggaggccgtc gagcgcgagg cgctccgcgc cgagcgtcga
    11161 ggagagctcc acgaggaccg cgtaggccgg gggccggccg gcgaacgggt cgcgcaggcg
    11221 cggctggtgc cggaagacgg gcgtgagcgc ggcggcgccg atcgcctcga aggcggtgag
    11281 cagctcgccg aggcgcgact cgagatgcgc gaggaggccc aggacggcgg ccccagagga
    11341 gaggccgacg aacgccgtca cccgctgggc cggacgcgga atgacgcgca tcacgacctt
    11401 cgtcacgacg ccgaagacgc ccgatgtgcc gacgaagagc tgacgcacgt ccaggcccgt
    11461 gttgttcttc cggagcgggc gcgtcaggtc gaggaccgtc ccgtcggcga gaactgcctc
    11521 gaggccgaga aggttctgcc ggacgtcgcc gtagcggagg agccgcgtgc cgcccgtgtt
    11581 cgtcgcgacc atcccgccga tcgaagggtc ggccccgagg tcgatcggga agaggaggcc
    11641 gtgctcctcg agcgcggcgt cgattcggct gaggaggacg ccgccgtcgg cggtcaccgt
    11701 cctctcgacc gggtcgacct cgagggtccg ggcgagccgc tcgaggctca ggacgagcat
    11761 ctcgccgctt ccgtcgggcg tcgaggctcc gacgaggccg gtgttcgccc cttgcgccac
    11821 gacgcgcagg ccgatcgagg atgcgaagcg caggaggcgc gagacctcct ccgtgctcga
    11881 cggccgcacg gcggcgagcg cccttccgcg gccgtagcgc caacccttct cgtagcgttc
    11941 gagctcgcgc ggctcgacga cgacggcgtt ctcgccgaag agggcgcgga gggaggcgac
    12001 cacgccctcg cgcggaacgg tcacgccggg cccgccgcgt ccccggctcc gtccacgacg
    12061 agcttccgcc gcagccggtc cgccagcccg gatagggaca tgtagaggca cgggacgacg
    12121 aagagcgtga ggagcgtcga gagcgtca
//
LOCUS       DAOONL010000096        12004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5904_length_12004_cov_20.479287, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000096 DAOONL010000000
VERSION     DAOONL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_12004_cov_20.479287"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLP50_12785"
     CDS             <1..510
                     /locus_tag="PLP50_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52465.1"
                     /translation="LPFLLLPLAFVRWGPLATALAGASTAAVALTTLVFPFVPPGFPL
                     PWGSFSAPLLAEGLVAPNLLHLVARPLAIVVPFALVLVAAYAAVGARRMGPFLLGALV
                     WTGAGLALPRLFPLAPPLVVQRGYVEEVYFGRDGATARAMPPGVPVPARLRERQALEK
                     TLPPPPWPF"
     gene            complement(455..2380)
                     /locus_tag="PLP50_12790"
     CDS             complement(455..2380)
                     /locus_tag="PLP50_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HPA52466.1"
                     /translation="MSPARTGRNELVLLATVFTVALGVRLLSVAAATEGGLRLGSPDA
                     YAHLRRATAITRSFPAVTVRDPWLNHPDGGVFIWPPAFDLLGGGISRLAFGADATRDE
                     VARVLAVLPPLLGALHVVPLFFLARRVLSRRRALLAACAYSVLPVAAVWSQFGHADQH
                     VAETLLLLLLLLALARTRVAGERRASFGRAFAAGAALAAMVLTWQGAVFAGVLSFAWA
                     AFALRPSVAVTFAVSSAGLTALGTLPYASGLELPFTFVSFGWFQPALLLAGASAVAAI
                     AAARSRGIARRACAGLGFLLLAATAPVAPDLARGLFGGARYVVSHTAGVARDEMARGG
                     YLSYPKDWIGLIAESRPLLRPPFLATLRNAVEESSPGIVLLPVAVALWVRRRPDRRLL
                     GLFGAVLLLMTLSQQRNGYYLAPFAALALAEALARFAPRGVMRPRPAAVAAASLLVVL
                     PGLPHYGRIVTYAGAPGTDLVETLERLGSLYPPPFDAAGHTVPEPGSAAGVFCPWSAG
                     HFVTALSGYPAAADPFGYGFRRQARLYTTPDDAEAARILREARCAWLFTTDLRAVLPA
                     YAAAAGRPPTPLAATFAVRVHESASPRPLPFLELAIDSRTAYRAPDGRVVPRFRVFRV
                     RTATEAAEGSSRAPDAP"
     gene            2407..>3417
                     /locus_tag="PLP50_12795"
     CDS             2407..>3417
                     /locus_tag="PLP50_12795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA52467.1"
                     /translation="MLRPGRRAAAAGAVLLALLGALLVVDHQRLDSATMDEPFHALAG
                     AEYALSGTYWANLEHPPLLKLVAGACAGLAGARPPRLPEPFQYRTAETPQRFLYENAI
                     PPERLLAAARLPFPLLFAALVLVAAAAAGRAAGALAGLGAALLLAFEPTLVAHAGLLH
                     TDVGAALGFLATVLAADAALRRPSWPRWGATGVLLGLSLAAKFTAVFLAPILLVLALA
                     RMLADRRSAGRLSVASLAGVALAGAVALPVLLGVYAVSMRNMPAAEAERSVRLFLSGR
                     LAAPATVERVAGLSRLCPPAGHYVAGLVGIAEQNRRGGGINVLCGELSVDGFPSYFPV
                     AFA"
     assembly_gap    3418..3517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3518..3997
                     /locus_tag="PLP50_12800"
     CDS             <3518..3997
                     /locus_tag="PLP50_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52468.1"
                     /translation="AAALAALGLLQAAETLAVHPHELSFFNWLAGGPAKGSLWLNDSN
                     LDWGQDLVRLARELERRGEADGLTVAYFGGAFPPHHLPGARLFDAASTPLTPGLYAVS
                     AYLLAAGPELMALRGDAARAAGYDRLRRTLAARGEPVGRVGYSILLYRIPGGEGAPP"
     gene            3994..5397
                     /locus_tag="PLP50_12805"
     CDS             3994..5397
                     /locus_tag="PLP50_12805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52469.1"
                     /translation="MKLSIIMPAYNERKTLREIVGRVLAVDLGGIEKELVIVDDGSTD
                     GTRDILRELDGKDGVRAVFQPRNLGKGAAVWTGMRASTGDLVVIQDADLEYDPREYSV
                     LLRPILDGEADVVYGSRFLGHPGGHRVLYFWHTVGNRMLTLMSNMFTNMNLTDMETCY
                     KAMTRSVVDRLDLQSKRFGIEPEITCKVARMRARVYEVPISYHGRTYEEGKKIGFKDA
                     LQAVWVILKFARWEAPRGDVGTMTLRRMERLAPYNAWLHDRFDAVLGQRVLEVGSGVG
                     NQTRFFVERERVVASDVEVHYVRELTARFGNRSNVRVASYRFPLSDADRAELVAERLD
                     SIVCLNVLEHIEDDAATLRDFASVLPRGGRLVLLVPSLKALYGTLDVHLHHFRRYERD
                     ELVKLVSGAGFEVETARFLNRPGVLGWWLNSRVLKRKVLPKGQLRAFRWLLPLLKLEE
                     KRPPSFGMSLLVLARKA"
     gene            5394..7706
                     /locus_tag="PLP50_12810"
     CDS             5394..7706
                     /locus_tag="PLP50_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52470.1"
                     /translation="MTPGRLARLEPIAAPAALAGAVALRYHEYLLGGSVVGRDAVFFF
                     VPLREVTSRLLAAGELPLWNDASGAGRALAANPNAAAFWPGTWLVPLLGTTGLFLLHV
                     LLVVLLSYAALRALGLSRPAGLAGGLVLLFSGVFQTVPLLFTTVASLAPIPLALAAVG
                     TLDPDDRRGARRRVATVGVALGLALLGGEPVVAAIGAAGAATLAALGALLDARGGSSR
                     RAGRRLVALAAAGLLSAGIAAVQLLPTAGELARSARGTQMRAEEGALFWSVRPARLLT
                     LLEPRLTGDPRAEDDADYWGAATFDAGNPYFHDLALGLLPLALVAAAAGDPRGRAALG
                     LAGLAACLSFGRFLPPVGAILAALPVFRYPEKWWLLATLGLVGAAAIGVDRLRDDRRA
                     ARRFTGASVMLAGVLFPAAALALAAPDRLAGLLRALSLAGDGVRGERVGSLLGPLLLA
                     ALATAVLLALLPALVRSGRLSPRVAAAIAAGLFLLDAGRRVAGSAPAGPAGFWVAAPA
                     EVAAAKEAAGEGRFFVDGADDVARATALARERDGLDPLRPITGAAWGLRYGGDNDIDR
                     MSTPEAVAASAALARLPWGEEKVRLLRSVGVTAARTDAPGPDPPGVRVTARFGSARIV
                     TLDGARPEHHFVRRARPAPATLVVPSSFDPLEETFVTGLDEEVAFGAGETTLVARSSR
                     HRRLRVRVAPPGGLLVVARSSDPSWTAFVDGRKVASLRADGFLTAVPVPSGEHEVVLA
                     YRNGRILAGLAVTLLSFGVAAAMAYGGRRA"
     gene            7703..9805
                     /locus_tag="PLP50_12815"
     CDS             7703..9805
                     /locus_tag="PLP50_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52471.1"
                     /translation="MTSVREICEKRPFAVALFVALVLLLVPYRVLLGPGVPSGRDLIP
                     YFYPLKTHLAEAVRAGEMPWIDRFRQGGLPLLAWPGVAAFDPGNLLFVLLPTAAAAKA
                     WMLLRVLTGSAGFAVFLRLSGLPPLSSALGGLAWGAAGITASAASFLSTSSAYAALPW
                     LAAALLRVRSRRDGRSLVLLAAATAFLLVASVPEPLLAASLLALVLLAGRGPSSGPLE
                     RGKTLLLWVAGGLLGTLLAAPVVATYLVTGAESIRGVRGALLPGFAEEGSLPPIRLAE
                     LLADGVVADWTRTSAAPGIPDYPYFPSLTPGRVAWTLALLGLLLGRGARLRATTIAAL
                     GVLLALGPATPVAEWISSLLPFATAIRYPEKWLVLFAFGIAWLVALGAGVLEKAVPAR
                     RLPVFGLLGLFLALDRSAITPRLLPTSEARMLDEAPAVLTALPVARVDTPPRILPIPG
                     TKRVPGAVLPTPAAGGRATAAWVLAWSYTRFGVASVFEMDYDAALPRAQLEWANLVED
                     APLGSPLAIALARSAGAEAVVVPDRGPGGLPVAGLRRIANPVSPFRFVRRVVREPDAL
                     AASSRFLRERAPVDLAVLSAPGVELVPSRGRILRVSDRPARLEIEVAVDGPEPAYLFV
                     ARPLVAARRATLDGRPVSVDDANVGFTGLSVPPGRHVVRLQPSGRWLIIAAVMSAVAL
                     LGLTFVRRRSPSRGGDVV"
     gene            9802..11229
                     /locus_tag="PLP50_12820"
     CDS             9802..11229
                     /locus_tag="PLP50_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA52472.1"
                     /translation="MSLSRNTRRRLALYGGAALVVTVVLAIPSVLRSRTPPVEHRAPD
                     LPEMRQREATRLLREYVRIDSQNPPGRTVETARFWAEKLGCEGIPFEIVGDDPERPIV
                     VARLAGRSRGEALLLLHHMDVFPVGDLSAWDTPPFAGADGGGDLVQYLCGRGTLDMKG
                     QGIADFLAIASLRRDGIVPERDLVFVAEPGEETYTPEIGIGWVLENRSDLLAGVTDAF
                     NEGGVNEVSGDRIARFGIEVLQKANVIATAHARDEPALKALRDLLERKMSEERFRVLD
                     EVQDFLAFVGPSRSDIWGHHMDDARSAVASGKLGEGIPEVYRALFRDMYYFGKVEPAP
                     SGKGVTMDVVATLLPGSSARARWEDMVRWAAPLGVRLTLRHVTPDSVPAPRRGRAWET
                     LHTVLALDPDEHADVGPYVLTGSYTNLAYLRARGIRSFGVSPFAVNLLDAVTIHSKNE
                     RIHLPSYLEGVERTARIVREYALQP"
     gene            11372..11584
                     /locus_tag="PLP50_12825"
     CDS             11372..11584
                     /locus_tag="PLP50_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52473.1"
                     /translation="MSDADVRALLASVDEVVIAKGRKAQTATAKGLALEALKGPTGSF
                     RAPMVRVGRRLLVGFHPETLEELLKA"
     gene            complement(11729..>12004)
                     /locus_tag="PLP50_12830"
     CDS             complement(11729..>12004)
                     /locus_tag="PLP50_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPA52474.1"
                     /translation="FKKLPAGTLEKLLADKAQLTKVLTYHVVAGRVMAADVVKLSEAK
                     TVEGSPVRIAVKDGKVKLNDANVVKTDVGASNGVIHVLDTVILPPAK"
BASE COUNT         1391 a         4421 c         4332 g         1760 t
ORIGIN      
        1 ctgccgttcc tcctcctgcc cctcgcgttc gtccgctggg gccctctcgc cacggccctc
       61 gccggcgcgt ccacggcggc cgtcgcgctc acgacgctcg tcttcccgtt cgtgccgccc
      121 ggattcccgc tcccgtgggg gagcttctcg gcgccgctcc tcgccgaggg gctcgtcgcg
      181 ccgaacctcc tccaccttgt cgcgcggcct ctcgcgatcg tcgtcccgtt cgcgctcgtc
      241 ctggtcgcgg cgtacgcggc cgtcggcgcg cggcggatgg ggccgttcct cctcggagcg
      301 ctcgtctgga ccggtgcggg cctcgctctc cctcgcctct tcccgctcgc gcccccgctc
      361 gtcgtccagc gcggctacgt cgaagaggtc tacttcggga gggacggggc gaccgcgcgg
      421 gcgatgcccc cgggcgtgcc ggtaccggcg cgcctcaggg agcgtcaggc gctcgagaag
      481 acccttccgc cgcctccgtg gccgttctga cccggaaaac gcggaagcgc gggacgacgc
      541 gcccgtccgg ggctcgatag gcggtcctcg agtcgatcgc gagctcgagg aacgggagcg
      601 gccggggcga ggccgactcg tggacgcgga cggcgaacgt cgcggcgagg ggcgtcgggg
      661 gacggccggc ggcggcggcg taggccggga ggacggcccg caggtcggtc gtgaagagcc
      721 acgcgcatcg cgcctcgcgg agaatccgcg cggcctcggc gtcgtcaggg gtggtgtaga
      781 gacgggcctg ccgccgaaag ccgtagccga acgggtcggc ggccgccggg tagccggaga
      841 gggccgtcac gaagtgcccc gccgaccacg ggcagaagac ccccgccgcg gagcccggct
      901 cggggaccgt atggcccgcc gcgtcgaacg ggggagggta gaggctcccg aggcgctcga
      961 gcgtctcgac gaggtcggtc ccgggcgcgc cggcgtaggt cacgatccgg ccgtagtggg
     1021 gcagcccggg gaggacgacg agaagcgagg ccgcggcgac cgcggccggc cgcggccgca
     1081 tcaccccgcg aggagcgaaa cgggccagcg cttcggcgag cgcgagcgca gcgaacgggg
     1141 cgaggtagta gccgtttcgt tgctgcgaga gcgtcatcag gaggagcacg gcgccgaaga
     1201 ggcccaggag cctgcgatcc gggcgtcgcc ggacccagag agccacggcg acggggagga
     1261 ggacgatccc cggggaggac tcctccacgg cgtttcggag cgtcgcgagg aacggcgggc
     1321 gaaggagcgg gcgcgactcg gcgatgaggc cgatccagtc cttcgggtag gagagatagc
     1381 cgccgcgcgc catctcgtcg cgggcgacgc cggccgtgtg actcacgacg tagcgggcac
     1441 cgccgaagag gcctcgcgcg aggtcgggcg cgaccggggc ggtggccgcg aggaggagga
     1501 agccgagtcc cgcgcacgcg cgacgcgcga tcccgcgaga tcgcgccgcc gcgatcgcgg
     1561 cgacggccga cgctccggcg aggaggaggg cgggctggaa ccacccgaag gaaacgaacg
     1621 tgaacggcag ctcgaggccg ctggcgtacg gcagcgtccc gagggcggtg aggccggcgg
     1681 aggagacggc gaacgtgacc gcgaccgacg ggcgcagcgc gaaggcggcc cacgcgaacg
     1741 agaggacccc ggcgaacacc gccccctgcc acgtcaggac catcgcggcg agggccgccc
     1801 cggcggcgaa cgcgcgaccg aacgaagctc gacgctctcc ggcgacccgc gtcctcgcga
     1861 gggcgaggag gagaagaaga aggaggaggg tctcggcgac gtgctggtcg gcgtgcccga
     1921 actggctcca gaccgccgcg accggcagga cggagtacgc gcacgcggcg aggagcgcgc
     1981 ggcgccgcga gaggacgcga cgggcgagga agaagagcgg gacgacgtgg agcgcgccga
     2041 ggaggggcgg gaggacggcc aggacgcgag ccacctcgtc ccgcgtcgcg tcggcgccga
     2101 aagcgagccg ggagatcccc ccgccgagga ggtcgaacgc gggcggccag atgaagacgc
     2161 ctccgtcggg gtggttcagc cacgggtccc gtacggtgac ggccgggaac gagcgcgtga
     2221 tcgcggtcgc gcggcgcagg tgggcgtacg cgtcgggaga gccgaggcgg agcccgccct
     2281 ccgtcgcggc ggcgacggag aggagccgca cgccgagagc gacggtgaac accgtcgcga
     2341 ggaggacgag ctcgttccgg ccggtccggg cggggctcac ccgccgagtc ttacacttcg
     2401 cgcgccatgt tgcgtccggg acgtcgggcc gccgcggcgg gggccgtcct cctcgccctc
     2461 ctcggggcgc tcctcgtcgt cgaccaccag cgactcgaca gcgccacgat ggacgagccg
     2521 ttccacgcgc tcgccggcgc cgagtacgcc ctgtcgggga cctactgggc gaacctcgag
     2581 cacccgccgc tcctgaagct cgtcgccggc gcctgcgcgg gactcgccgg ggcccggccg
     2641 ccccgcctgc cggagccgtt ccagtaccgg acggcggaga cgccgcagcg cttcctctac
     2701 gagaacgcga tcccgccgga gcggctcctc gccgccgccc gcctcccgtt cccgctcctc
     2761 ttcgcggcgc tcgtcctcgt ggccgccgcg gccgcggggc gggccgcggg ggcgctcgcg
     2821 gggctcggcg ccgcgctcct cctcgcgttc gagccgacgc tcgtcgcgca cgcggggctc
     2881 ctgcataccg acgtcggcgc cgcgctcggc ttcctcgcca ccgtcctcgc ggcggacgcc
     2941 gccctgcgcc gtccgtcgtg gccgaggtgg ggcgcgacgg gcgtcctcct cggcctctcg
     3001 ctcgccgcga agttcacggc ggtcttcctg gccccgatcc tcctcgtcct ggccctcgcg
     3061 cggatgctcg cggaccggag aagcgcgggg cgcctctccg tcgcctcgct cgccggcgtc
     3121 gccctcgcgg gcgcggtcgc cctcccggtc ctcctcggcg tctacgccgt gtcgatgcgg
     3181 aacatgcccg ccgccgaggc cgaacgctcg gtccgcctgt tcctgtcggg tcgcctcgcg
     3241 gccccggcga cggtggagcg ggtggccggc ctctcgcgcc tctgtccgcc ggcgggccac
     3301 tacgtcgcgg gcctcgtcgg gatcgccgag cagaatcggc gcgggggagg gatcaacgtc
     3361 ctctgcggcg agctgtcggt cgacggtttc ccgtcgtact tcccggtcgc cttcgcgnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gcggcgctcg cggccctcgg
     3541 gctcctccag gcggcggaga ccctcgccgt ccatccccac gagctctcct tcttcaactg
     3601 gctggcggga gggccggcga agggctccct ctggctgaac gattcgaacc tcgactgggg
     3661 acaggacctc gtccgtctcg cgcgcgagct cgagcggcgc ggcgaggcgg atggcctgac
     3721 ggtcgcctac ttcggcgggg ccttcccgcc ccatcacctc ccgggcgctc gcctcttcga
     3781 cgccgcgtcg acgccgctca cgcccggcct ctatgcggtc tccgcgtacc tcctcgccgc
     3841 ggggccggag ctgatggcgc tgcgcgggga cgcggcccgg gccgcgggct acgaccggct
     3901 ccggcggacg ctggccgcgc ggggcgagcc cgtggggcgc gtgggatact cgatcctcct
     3961 gtaccgcatt ccggggggag aaggcgcgcc gccatgaagc tctcgatcat catgcccgcc
     4021 tacaacgagc ggaagacgct ccgggagatc gtcggccgcg tcctcgcggt cgacctcggc
     4081 gggatcgaga aggagcttgt gatcgtcgac gacgggtcga ccgacggcac gcgcgacatc
     4141 ctccgcgagc tggacgggaa ggacggggtc cgcgccgtct tccagccccg caacctcggc
     4201 aagggggccg ccgtctggac cggcatgcgg gcgtccacgg gcgacctcgt cgtcatccag
     4261 gacgccgacc tggagtacga ccctcgcgag tactccgtcc tcctccgccc catcctcgac
     4321 ggagaggcgg acgtcgtcta cggctcgcga ttcctcggcc acccgggcgg ccaccgggtc
     4381 ctctacttct ggcacacggt cggcaaccgg atgctcaccc tcatgtccaa catgttcacg
     4441 aacatgaacc tgacggacat ggagacctgc tacaaggcga tgacgcgctc ggtcgtcgac
     4501 cggctcgacc tgcagtcgaa gcgcttcggc atcgagccgg agatcacctg caaggtggcg
     4561 cggatgaggg cccgggtcta cgaggtgccg atctcctacc acggccggac gtacgaggag
     4621 gggaagaaga tcggcttcaa ggacgccctc caggccgtgt gggtgatcct gaagttcgcg
     4681 cgctgggagg cgccgcgcgg cgacgtgggg acgatgaccc tgcgccggat ggagaggctc
     4741 gccccctaca acgcctggct ccacgaccgc ttcgacgccg tgctcggcca gcgggtcctc
     4801 gaggtcggct ccggcgtcgg gaaccagacg cgcttcttcg tcgagaggga gcgggtcgtc
     4861 gcctcggacg tcgaggtcca ctacgtccgc gagctgaccg cgcggttcgg gaaccgctcg
     4921 aacgtccgcg tcgcgtcgta ccgcttcccg ctctccgacg ccgacagggc ggagctcgtc
     4981 gccgagaggc tcgactcgat cgtctgcctg aacgtcctcg agcacatcga ggacgacgcc
     5041 gcgacgctcc gcgacttcgc gtcggtgctg ccgaggggcg gccgcctcgt cctcctcgtg
     5101 ccgtcgctga aggcgctgta cggaacgctc gacgtccacc tccaccactt ccgccgctac
     5161 gagagggacg agctcgtgaa gctcgtctcg ggggcggggt tcgaggtgga gacggcgagg
     5221 ttcctgaacc gtccgggcgt cctcggctgg tggctcaact cgcgggtcct gaagcggaag
     5281 gtcctcccga aggggcagct ccgcgccttc cggtggctcc tgccgctcct caagctcgag
     5341 gagaagcgcc ctccgtcgtt cgggatgtcg ctcctcgtcc tggcgcggaa ggcgtgacgc
     5401 cggggcgcct cgcccggctc gagcccatcg ctgcgcccgc ggcgctcgcc ggggcggtgg
     5461 ccctccggta ccacgagtac ctgctcgggg ggagcgtcgt cggccgcgac gcggtcttct
     5521 tcttcgtccc gctccgggaa gtgacctcgc gcctcctcgc cgcgggagag ctgccgctct
     5581 ggaacgacgc ctccggcgcc ggacgcgctc tggcggcgaa tcccaacgcc gcggcgttct
     5641 ggcccgggac gtggctcgtc cctcttctcg gcacgacggg cctctttctc ctccacgtcc
     5701 tcctcgtcgt tctcctttcg tacgcggcgc tccgcgccct ggggctttcc cgtcccgccg
     5761 gcctggccgg cggcctcgtc ctcctcttct cgggcgtctt ccagacggtc ccgctcctct
     5821 tcacgaccgt ggcctcgctc gccccgatcc cgctggcgct cgcagccgtc gggaccctcg
     5881 atcccgacga ccgccggggc gcgcggcgga gggtcgcgac ggtcggagtc gcgctcgggc
     5941 tcgcgcttct gggcggagag cctgtcgtcg cggcgatcgg cgcggccggc gccgcgacgc
     6001 tcgccgcgct cggcgccctc ctcgacgccc gagggggctc ctcccggcgc gcgggccgcc
     6061 gactcgtggc cctggcggcg gcggggctcc tctccgccgg cattgccgcc gtccagcttc
     6121 ttccgacggc cggcgagctc gcccggtcgg cgcgggggac gcagatgcgg gcggaggagg
     6181 gggcgctctt ctggtcggtt cgcccggcac ggctcctgac gctgctggag ccgcggctga
     6241 cgggcgatcc acgcgccgag gacgacgcgg actattgggg cgcggcgacg ttcgacgccg
     6301 ggaaccccta cttccacgac ctcgcactcg gcctcctgcc gctcgccctc gtcgccgccg
     6361 ccgccgggga cccgcgcggc cgtgcggcgc tcggtctcgc gggcctcgcc gcctgcctct
     6421 ccttcgggag gtttctcccg ccagtcgggg ctatcctcgc tgcgctgccg gtcttccggt
     6481 atcccgagaa gtggtggctt ctcgccacgc tcggcctggt cggcgcggcg gcgatcggcg
     6541 tcgaccggct gcgtgacgac cggcgggccg cacgccggtt caccggggcc tcggtcatgc
     6601 tcgccggcgt gctcttcccc gccgcggcgc tcgcgctcgc ggctcccgat cggctcgcgg
     6661 gcctgctccg ggccctgtcg ctcgccggcg acggcgtgag gggggaacgg gtcgggagcc
     6721 ttctcgggcc gctgctcctc gccgcgctcg cgacggccgt tctcctcgcg ctcctgccgg
     6781 cgctcgtgag gtcgggccga ctctcccccc gggttgcggc cgcgatcgcc gcggggctct
     6841 ttctcctcga cgcgggacgc cgggtggccg gctcggcgcc ggccggcccg gcgggcttct
     6901 gggtcgccgc tcccgccgag gtggccgcgg cgaaggaagc tgccggcgag gggcgcttct
     6961 tcgtcgacgg agcggacgac gtcgcgcggg cgacggcgct cgcgcgcgag cgcgacggcc
     7021 tcgacccgct ccgcccgatc accggcgccg cgtgggggct gcgttacggc ggagacaacg
     7081 acatcgaccg gatgtcgacg cccgaggcgg tggcggcgtc ggccgcgctc gcgcggctgc
     7141 cgtggggaga ggagaaggtc cggcttctcc ggtcggtcgg cgtaacggcg gcccggaccg
     7201 acgcgccggg gccggatccc ccgggcgttc gggtgacggc gcgattcggg agcgcccgga
     7261 tcgtgacgct ggacggcgcg cggcccgagc atcacttcgt gaggcgcgcc cgaccggctc
     7321 ccgcgacgct cgtcgtcccg tcctccttcg accctctcga ggagacgttc gtcaccgggc
     7381 tcgacgagga ggtcgccttc ggagccggcg agacgacgct cgtcgcgcga tcgagccgcc
     7441 atcgacgtct ccgggtccgt gtcgcgccgc cgggcgggct cctcgtcgtc gcgcgctcca
     7501 gcgatccatc ctggacggcg ttcgtcgacg ggcggaaggt cgcgagcctc cgcgccgacg
     7561 ggttcctaac ggccgttccc gtcccgtccg gagagcacga ggtcgttctc gcctaccgga
     7621 acggccggat cctcgccggc ctcgccgtga cgctcctctc cttcggcgtc gccgcggcga
     7681 tggcctacgg tgggaggcgc gcatgacgag cgtgcgcgag atctgcgaga agcgcccctt
     7741 cgcggtcgcc ctcttcgtcg ccctcgtcct cctcctcgtg ccgtaccgcg ttctcctcgg
     7801 ccccggcgtc ccttcgggtc gcgacctgat cccgtacttc taccctctga agacgcacct
     7861 ggccgaggcc gttcgcgccg gggagatgcc ctggatcgac cgctttcgcc agggcggtct
     7921 cccgctcctc gcctggccgg gcgtcgcggc cttcgacccc gggaacctcc ttttcgtcct
     7981 cctcccgacg gcggcggccg cgaaagcctg gatgctcctg agggtcctga cggggtccgc
     8041 cggcttcgcc gtctttctcc ggctctcggg tctcccgccg ctctcctcgg cgctcggtgg
     8101 cctcgcctgg ggcgcggccg ggatcacggc ctcggcggcg agcttcctga gcacctcctc
     8161 ggcgtacgcc gcgctgccgt ggctcgcggc cgcgctcctt cgggttcgct cgcggcgcga
     8221 cggacggagc ctcgtccttc tcgccgccgc gaccgcgttc ctcctcgtcg cgtcggttcc
     8281 agagccgctt ctcgccgcgt cgctcctcgc cctcgtcctc ctcgccgggc ggggcccgtc
     8341 ttcgggcccg ctcgagagag ggaagacgct cctgctctgg gttgccggcg gtctcctcgg
     8401 cacgctcctc gcggcgccgg tggtcgcgac ctacctcgtg acgggcgccg aaagcatccg
     8461 gggcgtccgg ggggcgctgc ttccgggctt cgcggaagag ggttcgctgc cgccgatccg
     8521 gctcgcggag ctcctcgcgg acggcgtcgt cgccgattgg acgaggacga gcgccgcccc
     8581 cgggattccc gactacccgt acttcccgtc cctgaccccg ggccgggtcg cgtggaccct
     8641 cgcgcttctc ggccttctcc tgggtcgcgg cgcccgcctt cgggcgacga cgatcgccgc
     8701 gctcggagtc ctgctcgcgc tcggtccggc gacgcccgtc gccgagtgga tctcgagcct
     8761 cctcccgttc gcgaccgcca tccggtaccc ggagaaatgg ctcgtcctct tcgcgttcgg
     8821 catcgcctgg ctcgtcgcgc tcggagcggg cgtcctggag aaggccgtcc cggcgcggcg
     8881 tctgcccgtc ttcggcctcc tcgggctgtt cctcgcgctc gaccggtccg cgatcacccc
     8941 tcggctcctg ccgacctccg aggcgcggat gctggacgag gcgcccgcgg ttctgacggc
     9001 gcttccggtc gcccgggtcg acacgccgcc gcggatcctc ccgatccccg ggacgaagcg
     9061 cgttcccggc gccgtcctcc ccaccccggc cgcgggcggt cgcgcgacgg cggcgtgggt
     9121 gctcgcctgg agctacacgc gcttcggcgt ggcctccgtc ttcgaaatgg actacgacgc
     9181 cgcgctgccg cgcgcccagc tcgagtgggc gaatctcgtg gaggacgctc ctctcggcag
     9241 tcccctcgcg atcgcgctcg cccgctccgc cggtgccgag gccgtcgtcg tccccgacag
     9301 gggaccgggc ggccttccgg tcgccggtct gcggcgcatc gcgaatccgg tctctccgtt
     9361 ccgcttcgtg cggcgcgtcg tgcgcgaacc ggacgctctg gcggcctcgt cccgcttcct
     9421 gcgcgaacgc gccccagtcg acctggccgt cctctcggcc cccggggtgg agctcgtccc
     9481 ctccaggggc cgcatcctcc gcgtctcgga cagacccgcg cgtctcgaga tcgaagtggc
     9541 ggtcgacggg ccggagcccg cctacctctt cgtggcccgg cccctcgtcg ccgcgcggcg
     9601 cgcgacgctc gacggccgcc ccgtcagcgt ggacgacgcc aatgtcggct tcaccggcct
     9661 atccgtgccg cccgggcggc acgtcgtccg tttgcagccc tccgggagat ggctgatcat
     9721 cgcggccgtc atgtcggccg tcgctctgct cggtctcact ttcgtccgga ggaggtcgcc
     9781 gtcgcgcggc ggagacgtcg tttgagtctc tcccggaaca cccgtcgccg tcttgccctc
     9841 tacgggggcg ccgccctcgt cgtgacggtc gttctcgcga tcccgagcgt cctccgttcc
     9901 cgcacgccac cggtcgagca ccgggcgccc gacctccccg agatgcggca gcgggaggcg
     9961 acccgcctcc tccgcgagta cgtgcggatc gactctcaga acccgccggg tcgcacggtc
    10021 gagacggcgc gcttctgggc cgagaagctc ggctgcgagg ggattccgtt cgagatcgtg
    10081 ggcgacgacc ccgagcgccc gatcgtcgtg gcccggctcg cggggcggag tcgcggggag
    10141 gccctgcttc tcctgcacca catggacgtc ttcccggtcg gggacctttc ggcctgggac
    10201 actcctccgt tcgccggcgc cgacggtggc ggcgacctcg tgcagtacct ctgcgggcgc
    10261 gggacgctcg acatgaaggg gcaggggatc gccgatttcc tcgcaatcgc gtcgctccgc
    10321 cgggatggga tcgtcccgga gcgggatctc gtcttcgtcg ccgagccggg cgaggagacc
    10381 tacacgccgg aaatcgggat cggatgggtc ctcgagaacc gttccgacct cctcgccggc
    10441 gtcaccgacg ccttcaacga ggggggcgtg aacgaggtca gcggggaccg gatcgcccgg
    10501 ttcggcatcg aggtcctcca gaaggcgaac gtcatcgcga ccgcgcacgc cagggacgaa
    10561 cccgccctga aggcgctgcg ggatctcctc gagcggaaga tgtccgagga gcggttccgg
    10621 gtcctcgacg aagtgcagga cttcctcgcc ttcgtcggtc cctcgcgatc cgacatctgg
    10681 ggacaccaca tggacgacgc ccggtcggcc gtcgcgtcgg ggaagctggg cgaagggatt
    10741 ccggaggtct accgggcgct ctttcgcgac atgtactact tcggaaaggt cgagccggcc
    10801 ccctcgggga aaggggtcac gatggacgtc gtcgcgacgc tgctgccggg cagctccgcg
    10861 cgtgcccgct gggaggacat ggtgcgatgg gcggcgccgc tcggcgtgcg ccttacgctt
    10921 cggcacgtca cccccgacag cgtcccggcg ccgcgccgcg gtcgcgcgtg ggagacgctc
    10981 cacacggtcc tcgcgctcga ccccgacgag cacgccgacg tcgggccgta cgtcctgacg
    11041 gggagctata cgaacctcgc ctacctccgg gcccggggaa tccgcagctt cggggtctcg
    11101 ccgttcgcgg tgaacctgct ggacgccgtg acgatccaca gcaagaacga gaggatccac
    11161 ctcccgtcgt acctggaggg ggtcgagagg accgcgcgta tcgtccgcga gtacgctctg
    11221 cagccctgag ggaggggaag gaggagacgc catggccaag gtcggcttct actatcagcg
    11281 gcccgggtga acgagctgcg gacgcacccg tgaggtgctc gaaaggacgc agacgaaggt
    11341 cgccgaggag cgctcggcga aggcggcacc catgagcgac gcggacgtcc gggcgctcct
    11401 cgcctcggtc gacgaggtcg tgatcgcgaa ggggcgcaag gcgcagaccg cgaccgcgaa
    11461 gggactcgcc ctcgaggcgc tgaagggccc gaccggcagc ttccgggccc cgatggtccg
    11521 ggtcgggagg aggctcctcg tcgggtttca tccggagacg ctcgaggaac tcctgaaggc
    11581 ctgaagggcg gccttcgctc cccgtggtga ggggttggtg aggggtcaag tccgtttccc
    11641 gtcgtggcgt gaatcggaaa gaggggaggg cgcgccattg cgcggcctcc cctctcagcg
    11701 tggggcggac ttccggcgat gcctgcggct acttcgcggg tggcaggatg accgtgtcga
    11761 ggacgtggat gacgccgttc gaggcgccga cgtccgtctt cacgacgttc gcgtcgttca
    11821 gcttcacctt gccgtccttc acggcgatcc tcaccgggga gccctcgacg gtcttcgcct
    11881 cggagagctt gacgacgtcg gccgccatca ccctcccggc gacgacgtga taggtgagga
    11941 ccttcgtcag ctgggccttg tcggcgagga gcttctcgag cgttccggcg gggagcttct
    12001 tgaa
//
LOCUS       DAOONL010000097        11958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5934_length_11958_cov_21.517937, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000097 DAOONL010000000
VERSION     DAOONL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_11958_cov_21.517937"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLP50_12835"
     CDS             complement(<1..815)
                     /locus_tag="PLP50_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPA52475.1"
                     /translation="MTREKSLTIDGFRVEIDGERNLLDVIRKAHIELPTFCYHSELSV
                     YGACRLCMVEVEGRGLVTACTTAPEPGMTVKTQTATVRELRKTNIELLLASGNHDCPT
                     CGKSGSCKLQSLAERLGVTEVRFKAPVEEKPLDLTSPGLVRDPNKCVLCGDCVRFCSE
                     IQGIGVLDFTHRGSRSQVSPAFGKGLFDVDCVGCGQCAAVCPVGAILVKSEVERVYAA
                     LQDPARTVVVQVAPAVRAAIGESFGAADDTFTMGRIVSALRRLGFDRVYDTSFT"
     gene            complement(827..2704)
                     /locus_tag="PLP50_12840"
     CDS             complement(827..2704)
                     /locus_tag="PLP50_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPA52476.1"
                     /translation="MEATRRDYLEAEKRFARRVVICAGTGCMANGALKVFKALHEQAR
                     ERGIRLEIELDYEDERDKAGLLTRSGCQGFCQMGPLLSIEPDGILYCGVKPGDVLEII
                     EGVESGRPVERLLYRVPATGQVCRGHGDIPFYAQQQRTVLKACGSLDPENLREYIARG
                     GYFAARKALTEMTDEQVCQEILEAGLRGRGGGGFPTGRKWELTRVQPKGKKYVVCNGD
                     EGDPGAFMDRSVMEGNPHAVLEGMMICARAIGADEGYVYVRAEYPLAVARVRKAVKDA
                     EAAGLLGDDIFGSGQSFRVHVMEGAGAFVCGEETALLASIEGRRGMPMPKPPFPAQSG
                     LNGKPTVINNVETLASVPLILANGAAWYRGIGTEKSPGTKTFALTGHVANTGLIEVPF
                     GATLRKIVFDVGGGVTRKDGAIDETAFKAVQIGGPSGGCLTREHLDLPMDFDGLLAMG
                     AMVGSGGLVVMNQETCMVQVAKFFMQFTQNESCGKCVPCREGTKQMLVLLDDITEGRA
                     TEATLPLLEQLARNVKIGSLCGLGKTAPNPVLSTLKHFRDEYEAHVRRKVCPSRACKS
                     LRTPEIDPALCKGCTLCGRRCPVGAIHGERKAPHTIDAATCIRCGACVAACKFNAISG
                     I"
     gene            complement(2725..3231)
                     /gene="nuoE"
                     /locus_tag="PLP50_12845"
     CDS             complement(2725..3231)
                     /gene="nuoE"
                     /locus_tag="PLP50_12845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPA52477.1"
                     /translation="MLTVETEAPETARQFTSVLRILESFDHDRSKLVPILQAVQEEYR
                     YLPEEVMTFVATALDIPPARVYGVATFYSHFALEPKGKYVVRICDGTACHVKGSQDIA
                     DALVESLKLPRGKRTTPDMLFTLETVSCLGACGLAPAVVVNDEVHGQMDPERTRALID
                     RIRAEEAQ"
     gene            complement(3397..4065)
                     /locus_tag="PLP50_12850"
     CDS             complement(3397..4065)
                     /locus_tag="PLP50_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPA52478.1"
                     /translation="MRVDSVPEATLRRLPSYYHYLLRLREKGQEATSAAQIGAALGVH
                     HTQVRKDLAVTGSQGRPKVGHRVDDLIASIEEFLNWNNVSDAFLVGAGHLGTALLGYA
                     GFERAGVRIVAAFDASPRRAGRKVQGVPVLPVEKLPDLARRMHIAIGILTVPADEAQA
                     VAEMMVGAGIQAIWNFAPIVLDLPEAVIVEDVALYSSLGVLSRKLAVRRKAASGKGSA
                     GTGP"
     gene            complement(4160..5119)
                     /locus_tag="PLP50_12855"
     CDS             complement(4160..5119)
                     /locus_tag="PLP50_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA52479.1"
                     /translation="MDHDPVYRGGRLDPQAEALLAAIAAEGPAPTASLTPVQARATFL
                     RPSWLGSPREMAAVRDLLIRGPAGPLRLRAYRPHGRPPLPVLVFFHGGGFVVGTLEEF
                     DAFCTLLADGAGCLVVSVDYRLAPERPFPAAAEDAFAALRWVGARAGEMDGDPARIAV
                     AGDSAGGNLAAGVALRARDEGGPALVQQVLISPWVDLSCTETESFRLFGRGGWLSAAS
                     LRWYRDHYLTDPAQASHPQASPCFARDLRGLPSALVLDAEFDVLRDQVRAFAARLREA
                     GNAVDYRRHAGQLHDFVVLPGLFEQATIAIDEVCASLRAAFRR"
     gene            complement(5136..5573)
                     /locus_tag="PLP50_12860"
     CDS             complement(5136..5573)
                     /locus_tag="PLP50_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPA52480.1"
                     /translation="MSGRLMELVSRAHLRWKRRVARDLAPYGVHPKQLFVLRKLREAG
                     SLLPSEIAALVFADRPTTTSMLATMERAGWITRRRDPGNGRRVIVEVTAAGRRKLASV
                     PARLWRTGRTAIDPEAGLSAPERAEAIRLLEKLNAWLDQAGDR"
     gene            5734..6099
                     /locus_tag="PLP50_12865"
     CDS             5734..6099
                     /locus_tag="PLP50_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HPA52481.1"
                     /translation="MGDPRVFFAAERTLLAWIRTGLTVMAFGFLVARFGLFLRVAAAQ
                     LGAEAHTPRFAPLSHAIGVLLVLAGTFSILVAAREHRAFLRTLPESDRPAGRDARLHE
                     RMATLLGVLGILLTLYLVL"
     gene            6174..6962
                     /locus_tag="PLP50_12870"
     CDS             6174..6962
                     /locus_tag="PLP50_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPA52482.1"
                     /translation="MSVAEWVSRFNDRYLYRLPGGARRTAPRVPINLQKATAGAVVFG
                     LMLHAGDFSLAAWLYLVLHGSYGLLWLAKDVAFPDPAWRRPQSLTSAVAVFVYPLGLY
                     YLAPLVMLTELGSAVPGGWGTTATLPAGIAAAAVACYAFGVFLHFGADGQKHFVLAHQ
                     RPRRLITDGFFAATRNPNYLGEILIYSSFCLLAQHWLPWAACATVWLQVFLPNMLRKD
                     RSMSRYPEHPAWVARTGLLLPSIPTLVRRLPDLFRTRSADGAES"
     gene            complement(7100..7426)
                     /locus_tag="PLP50_12875"
     CDS             complement(7100..7426)
                     /locus_tag="PLP50_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52483.1"
                     /translation="MENVLSPAVTESIRNAERDARTMGPDVRSELREAVARGHGLADR
                     LASDVRTAAGELSDRARGALSKSRRRASRAVDRVSDYADENTALVSLAVFGVGLLVGA
                     LAARRR"
     gene            complement(7497..8069)
                     /locus_tag="PLP50_12880"
     CDS             complement(7497..8069)
                     /locus_tag="PLP50_12880"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HPA52484.1"
                     /translation="MKNLSRNATTALFAAALLAAAPFAAAEATTMEKIDTEVSEALTA
                     IAVKARLITKLGGDGLAVKVEIHGNKAVLTGEVEKKESQELAKEVALSVAEVKEVDNR
                     VTQKPSERASTAEEVEAEIKDGILETRVKGALLSEVGRNALKIEVEATNGVVSLRGTV
                     PSTAISKASVAKAKSVPGVTKVVSLLLAAA"
     gene            complement(8132..8284)
                     /locus_tag="PLP50_12885"
     CDS             complement(8132..8284)
                     /locus_tag="PLP50_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HPA52485.1"
                     /translation="MLWTIFVILLVLWLLGMVSSYTLGGFLHVLLVLAIAVVLIRVIQ
                     GRDPIA"
     gene            8566..10041
                     /locus_tag="PLP50_12890"
     CDS             8566..10041
                     /locus_tag="PLP50_12890"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPA52486.1"
                     /translation="MARTTPALLLLLLGSLRAPAAPAPGEVARDPRVTQALSLLTAWF
                     DAEQAHRRLPGLSLAVVRDQELLYARGFGYAHLETKVPATPQTMYSICSISKLFTSVA
                     LMQQRDAGRLRLDDPVSKHLPWFSIGGMPVGAPPVTVFGLLTHSSGLPRESDVPYWTG
                     PGYPFPPAEKIRGKVGEQEMLYRPETVFQYSNLGLTLAGEVAAAAAGKPWAGLVKGGI
                     LDPLRMKDTEVEHLDRWRGNRLATGYTAPRRDGTREKVPPYEVRGIAPAAGMTSTVED
                     LGRFASWQLRLLGSGKTELLDVDTLREMQRVQFMDPGWKTSWGLGFQVWRNGERTFVG
                     HNGYCPGYQSQLLVQTDEKLATVFMTNTNGIDAQRYAQVAYDVVAPAVKKALDDPSGA
                     KPHVAAFARYAGRYDNWLAGEANVFPWDDGLALLPTPSERPLDALVKLKPAGEHRFRR
                     LRDDGSLGEEIRFEVDAQGRAIRLWRFSNSMERAAEPLPAR"
     gene            complement(10075..10671)
                     /locus_tag="PLP50_12895"
     CDS             complement(10075..10671)
                     /locus_tag="PLP50_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPA52487.1"
                     /translation="MSTSRSPKKEATYADLLALPENVIGEIVDGELIASPRPTAPHAR
                     ATSVLGGDLGPPFDRGRGGPGGWWIVFEPEVHLGRQVLVPDLAGWRRERMPAFPTDAF
                     FTLAPDWVCEVLSPSTQRLDRVRKLAVYLEEGVSWAWLVDPLARTLEVFESRGNRWTL
                     AGAWEGDAVVRAVPFDAVEIEIGGWWVPGAEPTADPRP"
     gene            complement(10727..>11958)
                     /locus_tag="PLP50_12900"
     CDS             complement(10727..>11958)
                     /locus_tag="PLP50_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52488.1"
                     /translation="DLTAGLKLLQIEAILAPPPPAEEDRAEREAFIAGLLGTAADAAE
                     RAKKLSQLTAGLTRDEIRALLAPGAADPAVAAAADAAERARKEVDRLISKRKREILER
                     ECFGLVEFVEPGHDFSVVGGMDEVKKDLLVLADNIREGRRSRVPMGILFTGPMGTGKT
                     FVAEAFAKECGLPTIKLKNFRSKWVGATEGNLERILTVIKAIGQVVVIIDEGDRAFGN
                     TDGEGDGGTSSRVIARIKEFMSDTQNRGRILFLVMTNRPDKLDVDLKRAGRLDRKIPF
                     LYSQAPEEVEAVAKALVKKNKVRSDVDLATIRDTFSAKLVGYSNADVEAVVLMANDDA
                     AREAGGDAPVTAEHFVKAASDYFPSRDAELLEYMELLAVFEASSRRLLPAKYAHMTPE
                     ELDVRLRLLRATVGTRR"
BASE COUNT         1646 a         4274 c         4195 g         1843 t
ORIGIN      
        1 gtgaaggacg tgtcgtagac cctgtcgaat ccgaggcgac ggagcgcgga gacgatgcgc
       61 cccatggtga aggtgtcgtc ggcggcgccg aaggactcgc cgatggccgc gcgcacggcg
      121 ggcgcgacct ggacgacgac ggtccgcgcg gggtcctgga gggcggcgta gacccgttcc
      181 acctcgctct tcacgaggat ggcgcccacc gggcagacgg cggcgcactg gccgcagccg
      241 acgcagtcca cgtcgaacag ccccttgccg aaagcggggg acacctggga gcgcgagccc
      301 cggtgcgtga agtccagcac gccgatcccc tggatctcgg agcagaagcg cacgcagtcg
      361 ccgcagagga cgcacttgtt ggggtcccgc accaggccgg ggctcgtgag gtccagcggc
      421 ttctcctcca ccggggcctt gaaacgcacc tcggtcaccc cgaggcgttc agccagcgac
      481 tggagcttgc aggagccgct cttgccgcag gtggggcaat cgtggttgcc gctcgccagg
      541 aggagctcga tgttcgtctt gcggagctcg cggaccgtgg cggtctgggt cttcaccgtc
      601 atgcccggtt cgggcgcggt ggtgcaggcc gtcacgagcc cccgcccctc gacttcgacc
      661 atgcagagcc gacaggcgcc gtagaccgag agctcgctgt ggtagcagaa ggtcggcagc
      721 tcgatgtggg ccttgcggat cacgtccagc aggttccgct cgccgtcgat ctcgacccgg
      781 aacccgtcga tggtcaggct cttctctctc gtcatggctg gttccctcag atcccgctga
      841 tcgcgttgaa cttgcaggcc gcgacgcagg ctccgcagcg gatacaagtg gcggcgtcga
      901 tggtgtgggg ggccttgcgc tcgccgtgga tggcgcccac cgggcacctc ctgccgcaga
      961 gcgtgcagcc cttgcagagc gccgggtcga tctcgggcgt ccggaggctc ttgcaggccc
     1021 ggctggggca gacctttcgg cggacgtggg cctcgtactc gtcgcggaag tgcttcagtg
     1081 tggacagcac cgggttcggc gccgtcttgc ccagcccgca gagggagccg atcttcacgt
     1141 ttcgggccag ctgctccagg agcggaagcg tcgcttccgt ggcccggccc tcggtgatgt
     1201 cgtccagcag gacgagcatc tgcttcgtcc cttcccggca ggggacgcac ttgccgcagc
     1261 tctcgttctg ggtgaactgc atgaagaact tcgccacctg gaccatgcag gtctcctggt
     1321 tcatcacgac caggccgccg ctccccacca tggcccccat cgcgaggaga ccgtcgaagt
     1381 ccatcggtag gtcgaggtgc tcgcgggtga ggcacccgcc ggagggaccg ccgatctgca
     1441 cggccttgaa ggctgtttcg tcgatggcgc cgtccttccg ggtcacgccg ccccccacgt
     1501 cgaagacgat cttgcggagc gtggctccga acggcacctc gatgaggccc gtgttcgcca
     1561 cgtggcccgt gagggcgaag gtcttggtgc cggggctctt ctccgtgccg atgccgcggt
     1621 accaggccgc gccgtttgcg aggatgaggg gcacggaagc gagcgtctcg acgttgttga
     1681 tgacggtggg cttgccgttg aggccgctct gcgccgggaa cggcggcttc ggcatcggca
     1741 tgccgcgcct gccctcgatg gaggccagca gcgcggtctc ctcgccgcag acgaaggccc
     1801 ctgcgccctc catgacgtgc acgcggaagc tctggccgct gccgaagatg tcgtcgccga
     1861 gcagcccggc cgcttccgcg tccttcacgg ccttgcgcac ccgggccacg gccagggggt
     1921 actcggcgcg gacgtagacg tagccctcgt cggcgccgat cgcgcgggcg cagatcatca
     1981 tcccctcgag gacggcgtgg gggttccctt ccatcacgga gcggtccatg aacgccccgg
     2041 ggtcgccctc gtcgccgttg cagacgacgt acttcttccc cttgggctga acgcgggtca
     2101 gctcccactt ccgtcccgtc ggaaacccgc cgccgccgcg accgcgaagg cccgcctcca
     2161 ggatctcctg gcagacctgc tcgtccgtca tctcggtcag cgccttgcgg gccgcgaagt
     2221 agccgccccg cgcgatgtac tcgcggaggt tctccgggtc gaggctgccg cacgccttca
     2281 gcaccgtgcg ctgctgctgc gcgtagaagg gaatgtcgcc gtggcccctg cagacctggc
     2341 cggtggccgg aacccggtag aggaggcgct ccaccggccg gccgctctcc acgccctcga
     2401 tgatctcgag cacgtctccg ggctttacgc cgcagtagag gatgccgtcc ggctcgatgg
     2461 agaggagcgg ccccatctgg cagaagccct ggcatccgct ccgggtgagg aggccggcct
     2521 tgtcccgctc gtcctcgtag tccagctcga tctcgaggcg gatgccccgt tcgcgggcct
     2581 gctcgtggag agccttgaag accttcaggg cgccgttcgc catgcagccc gtgccggcgc
     2641 agatcaccac gcggcgggcg aaccgcttct cggcctccag gtagtcccgg cgggtggctt
     2701 ccaggttcgt ctcgagccgg gcggtcactg ggcttcctcc gcgcggatcc ggtcgatgag
     2761 cgcccgggtg cgttccgggt ccatctgccc gtggacctcg tcgttgacga cgactgccgg
     2821 cgcgaggccg caggcgccga ggcagctgac ggtctccagg gtgaacagca tgtccggcgt
     2881 cgtccgcttc ccccggggga gcttcaggct ctccacgagg gcgtccgcga tgtcctggct
     2941 tcccttgacg tggcaggctg tgccgtcgca gatccggacg acgtacttgc ctttcggttc
     3001 cagggcgaag tggctgtaga aggtcgcgac gccgtagacg cgggccggag gaatgtccag
     3061 cgccgtggcc acgaaggtca tgacctcctc gggcaggtag cggtattcct cctgaactgc
     3121 ctggaggatc ggcaccagct tcgagcgatc gtggtcgaag gactccagga ttcggagcac
     3181 cgacgtgaac tgacgtgcgg tctccggggc ttcggtttcg actgtgagca tctgttcgcc
     3241 tcgttgccac aagggcaggc tgggtccggg gccctcgaaa ggagggcccg ctggtgagag
     3301 cttcgggcgt ggcccgtggg gccggtcctc gcgggacggg acccgggacc gggcgacggc
     3361 ggaggtgtcc ctcgggaccc tcctcgttga accggctcag ggccccgtgc cggcggagcc
     3421 ctttcccgac gccgccttcc ggcggacggc gagcttgcgg gagagcacgc ccaggctgga
     3481 atagagggcc acgtcctcga cgatgaccgc ctccggaagg tcgagcacga tgggggcgaa
     3541 gttccagatg gcctggatcc cggcgccgac catcatctcg gccaccgcct gggcctcgtc
     3601 ggcggggacc gtgaggatgc cgatggcgat gtgcatgcgc cgggcgaggt ccgggagctt
     3661 ctccacgggc aggacgggaa cgccctggac cttccttccc gccctcctgg ggctggcgtc
     3721 gaaagccgcg acgatccgaa ccccggcccg ctcgaagccc gcgtatccga gcagggccgt
     3781 gcccaggtgc cccgccccca cgaggaaggc gtccgacacg ttgttccagt tgaggaactc
     3841 ctcgatggac gcgatcaggt cgtcgacccg gtgccccacc tttggtcgtc cctggctgcc
     3901 cgtgacggcg agatccttcc ggacctgggt gtggtgaacc cccagcgccg cgccgatctg
     3961 ggccgcggaa gtcgcctcct ggcccttctc gcggaggcgg aggaggtagt ggtagtagga
     4021 aggaagacgt cgcagggtgg cttcaggaac cgaatccacg cgcatgcgcg cctccgggtc
     4081 tgagcggata tatcgagaaa aacatctcga taaaaaattc tagattaggc gctcctccag
     4141 gtcaagccct cccggcgagt catcgccgga aggccgctcg aagcgacgcg cagacctcgt
     4201 cgatggcgat cgtcgcctgt tcgaagaggc ccggcaggac gacgaagtcg tggagctgtc
     4261 ccgcgtgccg acggtagtca acggcattgc ccgcttcccg caggcgcgcc gcgaaggccc
     4321 ggacctggtc ccggaggacg tcgaactcgg cgtcgaggac cagcgccgac ggcaggccgc
     4381 ggaggtcccg ggcgaagcac ggggaggcct gcgggtgcga cgcctgcgcc gggtccgtca
     4441 ggtagtggtc gcggtaccac cgcaggctcg cggccgagag ccagcccccc cggccgaaca
     4501 ggcggaacga ctccgtctcg gtgcaggaga ggtcgaccca cggagagatg aggacctgct
     4561 gcacgagcgc cggcccccct tcgtctcgtg ctcggagcgc cacgcctgcg gcgaggttgc
     4621 ctccggcgct gtcgcccgcg acggcgatcc gtgcgggatc cccgtccatc tctccggcgc
     4681 gggcgccgac ccagcggagc gccgcgaacg cgtcctccgc tgccgcgggg aacggccgct
     4741 ccggagcgag ccggtagtcg acggagacca cgagacagcc ggcgccgtcc gcgaggagcg
     4801 tgcagaacgc gtcgaactct tccagcgtgc cgacgacgaa gccgccgccg tggaagaaga
     4861 cgaggacggg gaggggcggg cgtccgtgag ggcggtaggc tcgaaggcgc agcgggccgg
     4921 ccgggccccg aatcaggagg tcgcgcacgg ccgccatctc gcggggggat ccgagccagg
     4981 agggacgaag gaaggtcgcg cgggcctgca ccggcgtgag cgatgctgtc ggcgcgggtc
     5041 cttccgcggc gatcgcggcg agcagcgcct cggcctgcgg gtcgaggcgg ccacccctgt
     5101 agacggggtc gtgatccatc gaaggacgac ctccttcagc ggtccccggc ctggtcgagc
     5161 caggcgttca gcttctcgag cagccgtatc gcctcggctc gctccggggc cgagaggccg
     5221 gcttcggggt cgatcgccgt ccgtcccgtc ctccagaggc gggccggaac ggaggccagc
     5281 ttcctccgac ccgccgctgt gacctcgacg atcacccgcc tgccgttccc ggggtcgcgc
     5341 cggcgggtga tccagccggc ccgctccatc gtcgcgagca tggaggtcgt ggtcgggcga
     5401 tcggcgaaga cgagcgccgc gatctcgctc ggcaggaggc tccccgcttc ccggagcttc
     5461 cggaggacga agagctgctt cggatggacg ccgtacggag ccaggtccct cgcgacgcgg
     5521 cgcttccatc ggaggtgggc gcgagagacg agctccatca accggccgga cacgattaga
     5581 agtctaacta tcgacccgga cggccgcaag cgcctgctgc gtttcggggg ccatctctcc
     5641 gggcgaacgg ccggacgggg tgagccgtct ccggcgaagg aacggacgag gagggggcgc
     5701 gtcccgcgcg gcgccccgtg tactctcgcc cgcatgggcg atccccgcgt cttcttcgcc
     5761 gccgagcgga ccctcctcgc gtggatccgg accggcctga ccgtgatggc gttcggcttc
     5821 ctcgtcgccc gcttcggcct cttcctccgg gtcgccgccg cgcagctcgg ggccgaggcg
     5881 cacacgcccc gtttcgcgcc cctttcgcac gcgatcgggg tcctcctcgt cctcgccggg
     5941 accttctcga tcctcgtcgc ggcgcgcgag caccgcgcct tcctccggac gctcccggag
     6001 agcgaccgtc ccgccgggcg cgacgcgcgc ctccacgagc ggatggcgac gctcctcggc
     6061 gtcctcggga tcctcctcac gctctacctc gtcctgtagc gaccggcgga gttgacgcgc
     6121 cgacggatcg ggtgtgttct cggaccgcac gggccgggag acgggaggag gccatgtcgg
     6181 tcgcggagtg ggtctcgcgc ttcaacgacc ggtacctcta ccgtctcccg gggggagcgc
     6241 gccggacggc gccgcgcgtc ccgatcaacc ttcagaaggc gacggcggga gccgtcgtct
     6301 tcgggctgat gctgcatgcg ggcgacttca gcctcgccgc gtggctctac ctcgtcctcc
     6361 acgggtcgta cggactcctg tggctcgcga aggacgtcgc cttcccggac cccgcctggc
     6421 gccgcccgca gtcgctcacc tcggccgtcg ccgtcttcgt ctacccgctc ggcctctact
     6481 acctcgcccc gctcgtcatg ctcaccgaac tcgggagcgc cgtgccggga ggctggggaa
     6541 cgacggcgac ccttcccgcc ggcatcgccg cagcggccgt cgcctgctac gcgttcggcg
     6601 tcttcctcca tttcggggcc gacggccaga agcacttcgt cctcgcgcac cagcgcccgc
     6661 ggcggctgat cacggacggc ttcttcgcgg cgacccgaaa cccgaactat ctcggcgaga
     6721 tcctcatcta ctcctctttc tgtctcctcg cgcagcattg gctcccctgg gccgcctgcg
     6781 cgacggtctg gctccaggtc ttcctgccga acatgctccg caaggatcgg tcgatgtcgc
     6841 gctacccgga gcacccggcg tgggtcgcgc ggaccggact cctcctccct tcgattccga
     6901 cgctcgtgcg ccggcttccg gacctcttcc ggacgcggag cgcggacggc gcggagtcct
     6961 gaaggtccgc acccctcccg cactcgcgcg acgacgacac gcgccgacgg gctcgcgccc
     7021 gcgcatcatt cgacgcgcgg gcgcggcgac gtcgtgccgg tgtctccgga cgggcggact
     7081 cgggaggacg agccggccgc tacctccggc gggccgcgag ggccccgacg aggagcccga
     7141 cgccgaagac ggccagggag acgagcgcgg tgttctcgtc cgcgtaatcg ctcacgcggt
     7201 cgacggctcg cgaggccctc cggcggctct tcgagagcgc gcctctcgca cggtcggaga
     7261 gctccccggc ggccgtccgg acgtcggagg cgaggcggtc cgcgaggccg tggccgcggg
     7321 cgaccgcctc ccggagctcg ctcctcacgt ccggacccat cgtccgggca tcacgttcgg
     7381 cgtttcggat ggattcggtg acggcggggc tgaggacgtt ttccatggga gtcctccggt
     7441 tcgtcatttc gcggggtcgg cgtgattcgg gagggaccgg cggcccctcc cccgggtcag
     7501 gcggccgcca gaagcaggct gacgaccttc gtcacgcccg gaacggattt cgccttcgcg
     7561 accgaggcct tcgagatggc ggtggagggc accgtgccgc ggaggctcac gacgccgttc
     7621 gtcgcctcga cctcgatctt cagcgcgttg cgtccgacct ccgagaggag ggcgcccttg
     7681 acccgggtct cgaggattcc gtccttgatc tcggcttcga cctcctccgc cgtcgacgcg
     7741 cgctcggacg gcttctgcgt gacgcggttg tcgacctcct tgacctcggc gacggagagc
     7801 gcgacctcct tggcgagctc ctggctctcc ttcttctcga cctcgccggt gagcacggcc
     7861 ttgttgccgt ggatctcgac cttgacggcc aggccgtctc cgcccagctt cgtgatgagc
     7921 ctggccttca cggcgatggc cgtgagcgcc tcggagactt ccgtgtcgat cttctccatc
     7981 gtcgtcgcct cggcggccgc gaagggggcc gcggcgagga gcgcggcggc gaagagggcg
     8041 gtggtggcgt tgcgtgagag gttcttcatg gcttctcctt tcggtcgaag gacgcgacgg
     8101 gcgcgcggga gtcggggacg cgccgccccc gtcaggcgat cgggtcgcgg ccctggatca
     8161 cccggatcag gacgaccgcg atcgcgagaa cgagcagcac gtggaggaag ccgcccagcg
     8221 tgtaggagct caccatgccg agcagccaga gcacgagcag gatgacgaat atggtccaga
     8281 gcatctctct ctcctttccg gttccgtccg gactcgagac cgcaggagcc gtgccaggcc
     8341 cggagccccg ccggaacgcc gttcgcgggc cgtccggccg ggagagggag gaagatcttt
     8401 ccgacgcctc ttcaccgcga ggacactttt tcaaggcgag ccgtgagacg gacgcgtccc
     8461 gatcccctcg gcgttccgcg gaccgacggc ctcgttcctc gagcggcggg cttgacggcc
     8521 ggcgcccacc ggccgatcgt cgtctccccg gcgggaggtg acgcgatggc gagaacgacc
     8581 ccggccctgc tccttcttct cctcggatcc cttcgcgccc cggccgcgcc cgcgcccgga
     8641 gaggtcgcac gcgacccacg ggtcacgcag gcgctctcgc tcctgaccgc ctggttcgac
     8701 gccgaacagg cccaccggcg gctgccgggc ctctcgctcg ccgtcgtccg cgaccaggag
     8761 ctcctctacg cgcgcggctt cgggtacgcg cacctcgaga cgaaggtgcc cgcgacgccg
     8821 cagacgatgt actcgatctg ctccatctcg aagctcttca cgagcgtcgc cctgatgcag
     8881 cagcgcgacg ccgggcggct ccggctcgac gacccggttt cgaagcacct gccctggttc
     8941 tcgatcggcg ggatgcccgt cggcgcgccg ccggtgaccg tcttcggcct cctgacccac
     9001 tcctcgggtc tcccgcgcga gtcggacgtc ccgtactgga ccgggcccgg ctaccccttc
     9061 cctcccgccg agaagatccg cggcaaggtc ggcgagcagg agatgctcta ccggcccgaa
     9121 acggtcttcc agtactcgaa cctcgggctg acgctcgcgg gcgaggtcgc cgccgccgcg
     9181 gcagggaagc cgtgggccgg gctcgtgaag ggcgggatcc tcgacccgct caggatgaag
     9241 gacacggagg tggagcacct cgaccgctgg cgcgggaacc ggctcgcgac cggctacacc
     9301 gcgccccgca gggacgggac gcgcgagaag gtgccgccgt acgaggtgcg cgggatcgcg
     9361 ccggccgccg ggatgacctc gaccgtcgag gacctcggcc gcttcgcctc ctggcagctc
     9421 cgcctcctcg ggagcgggaa gaccgagctc ctcgacgtcg acacgctccg cgagatgcag
     9481 cgggtgcagt tcatggaccc ggggtggaag acgagctggg ggctcggctt ccaggtctgg
     9541 cggaacgggg agaggacatt cgtcggccac aacggctact gccccggcta ccagagccag
     9601 ctcctcgtcc agacggacga gaagctcgcg accgtcttca tgacgaacac gaacgggatc
     9661 gacgcgcagc gctacgcgca ggtcgcctac gacgtcgtcg ccccggccgt gaagaaggcg
     9721 ctcgacgacc cgtcgggggc gaaaccccac gtcgccgcgt tcgcgcgcta cgccgggcgg
     9781 tacgacaact ggctcgccgg agaggcgaac gtcttcccgt gggacgacgg cctcgcgctc
     9841 ctcccgaccc cctccgaaag gccgctggac gccctcgtga agctgaagcc cgccggcgag
     9901 caccgcttcc ggcgcctccg cgacgacggc tcgctcggcg aagagatccg cttcgaggtc
     9961 gacgcgcagg gccgcgccat ccgcctctgg cgattcagca actccatgga gcgggccgcg
    10021 gagccgctcc cggcgaggta gccggccggc ggcggacctt cacgttcagc cgggtcaggg
    10081 ccggggatcg gccgtcggtt ccgcgcccgg gacccaccag ccgccgatct cgatctccac
    10141 cgcgtcgaac gggacggcgc ggacgacggc gtcgccctcc caggcccccg cgagtgtcca
    10201 gcggttcccg cggctctcga agacctcgag cgtccgggcg agcgggtcga cgagccacgc
    10261 ccaggacacc ccctcctcga ggtagaccgc gagcttgcgc accctgtcga ggcgctgggt
    10321 ggaaggggag aggacctcgc agacccagtc gggggcgagg gtgaagaacg cgtccgtcgg
    10381 gaaggcgggc atccgctccc gccgccagcc ggcgaggtcg gggacgagaa cctgccgccc
    10441 gaggtgaact tccggctcga agacgatcca ccagcccccc ggcccgcccc gtccccggtc
    10501 gaacggcggc ccgaggtccc ccccgagaac cgaggtggcc cgggcgtgcg gcgccgtcgg
    10561 ccgcggggag gcgatcagct ccccgtcgac gatctcgccg atcacgttct ccgggagggc
    10621 gaggaggtcg gcgtacgtcg cctccttctt cgggctccgg ctggtcgaca cgggggaagt
    10681 ctagcgcccc cgcccacctc ctccgcgtcc ggcacgccgg cgcggtctac ctccgcgtcc
    10741 cgaccgtcgc cctcagcagc cggagcctca cgtcgagctc ctccggcgtc atgtgggcgt
    10801 acttggccgg aaggagcctg cggctcgagg cctcgaagac ggcaaggagc tccatgtact
    10861 cgaggagctc cgcgtcgcgc gagggaaagt agtcggaggc cgccttcacg aagtgctcgg
    10921 cggtgacggg cgcgtcgccc cccgcctcgc gggcggcgtc gtcgttcgcc atcaggacga
    10981 ccgcctcgac gtcggcgttg ctgtagccga cgagcttcgc ggagaaggtg tcgcggatcg
    11041 tcgcgaggtc gacgtccgac ctcaccttgt tcttcttcac gagcgccttc gcgaccgcct
    11101 cgacctcctc gggcgcctgc gagtagagga acgggatctt ccggtcgagg cgccccgcgc
    11161 gcttcaggtc gacgtccagc ttgtcggggc ggttcgtcat gacgaggaag aggatccggc
    11221 cccggttctg ggtgtcggac atgaactcct tgatccgcgc gatgacgcgg ctcgaggtcc
    11281 ctccgtcccc ttcgccgtcg gtgttcccga acgcccggtc cccctcgtcg atgatgacga
    11341 cgacctgccc gatcgccttg atgacggtca ggatccgctc gaggttcccc tcggtcgccc
    11401 cgacccactt ggagcggaag ttcttcagct tgatcgtcgg gaggccgcac tccttcgcga
    11461 acgcctcggc gacgaacgtc ttccccgtcc ccatcgggcc ggtgaagagg atccccatcg
    11521 ggacgcggct ccgccgcccc tcgcggatgt tgtcggccag gacgaggagg tccttcttca
    11581 cctcgtccat cccgccgacg acggagaagt cgtgccccgg ctcgacgaac tcgacgaggc
    11641 cgaagcactc ccgctcgagg atctcccgct tcctcttcga gatgaggcgg tcgacctcct
    11701 tccgcgcccg ctcggccgcg tcggccgcgg ccgcaacggc cgggtcggcg gcgccggggg
    11761 cgaggagggc gcggatctcg tcgcgcgtga ggcccgcggt gagctgggag agcttcttgg
    11821 cccgctcggc agcgtcggcc gcggtgccga ggaggccggc gatgaaggcc tcgcgctcgg
    11881 cgcggtcctc ctcggcgggc gggggcgggg cgaggatcgc ctcgatctgg aggagcttca
    11941 ggccggccgt caggtcgg
//
LOCUS       DAOONL010000098        11919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_5959_length_11919_cov_21.046022, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000098 DAOONL010000000
VERSION     DAOONL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5959_length_11919_cov_21.046022"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..378
                     /locus_tag="PLP50_12905"
     CDS             202..378
                     /locus_tag="PLP50_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52489.1"
                     /translation="MPGPGGRTRWSSPPAEDGLERADAVVERITGEKPLAACATIVAA
                     ATNVGSCVPTSGVR"
     gene            556..3504
                     /locus_tag="PLP50_12910"
     CDS             556..3504
                     /locus_tag="PLP50_12910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA52490.1"
                     /translation="MSASSLEALDPRKAITQYPLDHWRTRDGLPLNSVRAFLQRRDGY
                     LWLGTQEGLARFNGLRFTRIAIEHPAGERFPDVRSLLEDRGADLWVGTYGSGVARLRD
                     GTWSVYSTKDGLPADSVNALRLDREGRIWVGTSGGLARIELGRDGEPRISSVPELRGI
                     AVNAICEVERGVLWIGTAHAGVLELRAGALRDISPSLPLRGRNVTAILADREGGRWLG
                     MSPLGLVRVAGASPDQSWDVEEVQWLLQDRDGNVWAGTIAGVLRIFEGRVFTLTTREG
                     LDDDLSLTGYEDRDGSIWIGTSTVGVHRLRDSRFTPITEKEGLSARFVRALLPSSDGA
                     LWIGTYGGGACREKDGRIDCFDTSAGLSNNYVRSIAQTRDGAIWVGTLNGLNRIAGGS
                     ITRFGLADGLLAPFVRCVFVDRRGDLWIGLDSGDVHVWNGGDPSRARTHRGVADKGIV
                     TMREDPDGRLWIGTYGDGLHVRENGQFRRVTATNGTLPPYVYCIWEKDGELLLGTNAG
                     LVSLTGTRIGPAAKDEAYTGNIYGIVDDGVGSLWFTSNQGVFRVPESAIAELRSGSAL
                     RDVGVQYDEADGMPGAECNGGFPAACRRPDGTVLFPTTRGVAAYRPDTKGFRRPPPPV
                     VIETVDLDEERIEIARAGSVRLPSGKRRLRFGFVALDYLEPRKTRYRYRLEGFDERWH
                     PTNQDPEAIYTNLPPGAYTFRVVASDGEGGWNPAGASFAFEVLPRFTQTIPFYALCVL
                     GVVASAVGGHRLRVRRMKVRERELTRLVDERTRDLERANLELAQLSLTDRLTEIANRR
                     ALEQFLDREWGRAVRNGLSFSLLMVDVDCFKAFNDRYGHPAGDECLRGVARALRSSAR
                     RPGDLAARWGGEEFVVVLSETDAEGAVVVAERIRAAVEDLQIAHEASPVSSHVTISVG
                     CATGIPGRGREWTSLVRAADEALYRSKGNGRNRVEAGRLDSPDDPTGDVSAPGPARAP
                     AASEGSGTIG"
     gene            complement(3581..4561)
                     /locus_tag="PLP50_12915"
     CDS             complement(3581..4561)
                     /locus_tag="PLP50_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPA52491.1"
                     /translation="MTDLQTTWFLLVGVLLVGYSVLDGIDGGVGTLVLSRSGEDETRR
                     RRLATLGPATLGNELWLVGGLAALFAAFPPVREAVASAFGALLGALALAFVLRAVVLG
                     KGRASGAVDRPRDVAFGALSALAAVLLGLMLGNVLLGLPLDEAGRFAGAFGDLLSPFA
                     LVVAVETVALFALQGACWLNLRVGEGEDLVLRAWVTAVALHGLTGVFSLFAAPHLWEP
                     YHHPLTWIAPVVMLASLVALPYFVKAGRPALALGSSSLSIAALWGIVGQGLYPALVPA
                     RGGAGESLTIAHAAASPDALTSLLVVLLVALTLVAGYSAFVFLRLRRTAV"
     assembly_gap    4783..4882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4924..6576)
                     /locus_tag="PLP50_12920"
     CDS             complement(4924..6576)
                     /locus_tag="PLP50_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA52492.1"
                     /translation="MRSTRLALVVPMALAALTCGSSPSDREVVPAPSRAASAGWDHEW
                     ARGAVFYEVFVRSFQDSDGDGIGDLPGLISRLDDLNDGDPATTTDLGVDALWLMPVFE
                     SPSYHGYDTTDYLKIEPDYGTNADFLRLLDEAHRRGMKVIVDLVVNHSSSQHPWFVSA
                     ASSPASPYRDWYVWSATDPGWKQPWGGNAGTWHRSGDAFYYGVFWGGMPDLNWRNASF
                     RGEMRRISALWLERGVDGFRLDATRYLVETGAGGGQADTPETHAALKAFAAHVRGVKP
                     QAFVVGENWADTAVIATYYGSKEVPGGDELPASFNFPLSDLVLKAVASGNAAPVAAKL
                     EEVARVYPAGAIDAPFLTNHDQVRLATQLGRNAARLRNAAAILLTLPGAPFLYYGEEV
                     GLENGPTGGDESKRTPMPWDATPGGGFTTGTPWFAFAPGRETANVAAQRNDPSSLLSR
                     YRSLIRARTSSEALRKGEITLLSARSGVTPFLAFLRASAGETVLVVHNLSDSFQSAGP
                     FAVPGGSFERVFADPGAADPSGAPGAIRVYLPGRGTGIWRVR"
     gene            6627..7469
                     /locus_tag="PLP50_12925"
     CDS             6627..7469
                     /locus_tag="PLP50_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52493.1"
                     /translation="MSGFDAARLGALVHDLLAVVRRRPADLLPFEEVRDRLKLRRVVD
                     RGTSDVPLEKIVGTFGRRGEFNRVFLPRSESLRERWEDVKDLAEGAAGFPPVELYLVG
                     DAYFAVDGHHRVSVARSLGARTIEAHVLEFLTPVPLGPDASLEDVLRKEGLADFLEAT
                     GLVPSSPDEFRVTHPGDYAEMLEHVAVHRYFLGLELSRCTSWEEAVRSWYETFFRPMT
                     ETIRASGVLADFPGRTEADLYLFVARHLHALRERWGDGIAPARAVKHLRLQTKRNGSR
                     KAKG"
     gene            complement(7476..9002)
                     /gene="malQ"
                     /locus_tag="PLP50_12930"
     CDS             complement(7476..9002)
                     /gene="malQ"
                     /locus_tag="PLP50_12930"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPA52494.1"
                     /translation="MRTRAAGLLLHPTSLPGRFGIGDLGPECDAFLDWAASAGQSVWQ
                     VLPLGPTGFGNSPYGTLSAFAGSPMLLSPELLVADGLLPASALDGAPGFPEHHVDFDR
                     VIPWKEGLLREAWRRWKTDAPSALRAGLQGFAEASAHGFWLRDWALFMALKGRYGGAA
                     FREWPEELRLRRPAALARAERELAEETSWHAFLQFLFFRQWERVKGEAHGRGIRILGD
                     VPIYVSGDSADVWAHPEIFELDAEGEPVRVAGVPPDYFSETGQLWGNPLYRWDVLEKD
                     RYRWWVERMRANLRLTELLRLDHFRGFAGYWAVPAGAETAIAGSWQEGPGRKLFDALA
                     AELGELDLVAEDLGEITPDVKALVAALDLPGMKILQFAFGGDDEDYQPHNHVPNGVVY
                     TGTHDNDTTRGWWEKADDATRHRVRAYLGGDGTDPIGIFVRAAYASVARLAVLPVQDV
                     FGLGSEARMNTPGKGADNWAWRARKWDFNGERAAWLRELATLTGRFRPGGAGNGGNGG
                     "
     gene            9066..11285
                     /locus_tag="PLP50_12935"
     CDS             9066..11285
                     /locus_tag="PLP50_12935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52495.1"
                     /translation="MKRGRLSLLAPAAVLLLAAAGVFSGCRGARAGDGPGTAPPTAGS
                     GTLLLTGELAAVRSADLFVPETPLWELQLRFLEEDGALVKTGDRVAEFDAGNLASELE
                     QKRIAAIEAETELVRFDADREVAELGREQAVRARTTDLEKARLDADLPAELRSRRDHE
                     EKQLALRRARTELAKAGEELAGSSAAADADRATKLIALERARAEREKLEAAVAALTLH
                     APKDGVFVVAENPRERKKFLTGDTLWPGLALARIPDPGALRVDALLFDVDDGRVRAGD
                     AAVVVPDTFPDRRLPARVASVAPVAQPIGRGSPRMAFRVQVDVTGGELDGLRPGMSVR
                     VETPPAPTRSVAADPSRVPLPPPDPASARQAKAEMRDLVLSVELKGELAALDGEPIGP
                     TRLPEVWDYRISFLAAEGSEVKKGQPVLRFDTTALSQQLEEKRAEAESAGKQIDRRRR
                     TLALARADLELQKAQAEARRAKAALKVDVPPEISGAIELTKARLDLALAEAESASVTR
                     RLEAAERAARAELSILEGRRRRAALRAGELTTAIGKMTVPAPRAGTVLLIPSWNGDKK
                     KVGDRCWYGEKILEVPDLARLGVQGEVDEADAGLVREGAAVSLRLDAHPDSEVRGKVL
                     RIQRTVQRASPSVPLKVARLSVVLEEVDPKKMKPGMRVRGRVETGRVARALAVPADAV
                     VPTEAGPFVWVPRGSGARPVAVKVGRRNGEWVEVLSGLTEGNAVLRVARPAPKGAAS"
     gene            11282..>11919
                     /locus_tag="PLP50_12940"
     CDS             11282..>11919
                     /locus_tag="PLP50_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52496.1"
                     /translation="MRRRALVALAAALLAAAALGARLLRPRTADARVPTFVVKAAKLE
                     RTVRAEGVLRAVDATPVRVPGNDVSLKIGWMIEDGTPVKAGDVVVRLDPTQTTKLLAD
                     GRAERATADRRGDKERAEAGALIANLRRDADQARDERSTAETFQSRDPELFSRHEIIE
                     ADIDVSLATQKERHAVDSKGEREALARTGRELVALEGRTAQLKIDKAERELAA"
BASE COUNT         1693 a         4267 c         4354 g         1505 t
ORIGIN      
        1 cgatcggcgc gaagggcttc gcgaacctga tgaccgagcc cgccttcgag gaggtcccga
       61 agatcctcga gaccccgaag gacgaggagg ggaagtggga ccgcgagggt ctcgcggccc
      121 tccgccggct cgcgcgacgg aagagcacgg ggtaaccggg cgacgttcca cgcggccgat
      181 cgctccttta cccgggacgc cgtgccgggc ccgggcgggc ggacgcggtg gagctccccg
      241 cccgccgagg acggcctcga gagagccgat gccgtcgtgg agcggatcac cggcgagaag
      301 ccgctcgcgg cctgcgcgac gatcgtcgcc gcggcgacga acgtcgggtc gtgcgtcccg
      361 acgtccggcg tgcgctgatc gcggggcccc ggcggacgag ggcgaccgcc cggcgcaccg
      421 gaagaggctg ccccgacgga cgggtcgagg ctacgatggg cgggaggagc gcagccggga
      481 gtgaggaaag gcgcccgaga ggcccggacg tgccgcggaa gccgatggct cgcggcgggt
      541 gggctgatcg ccctcttgtc ggcctcctcc ctcgaggcgc tcgatccccg caaggccatc
      601 acgcagtatc cgctcgatca ttggaggacg cgcgacggcc tccccctcaa ctccgtccgc
      661 gcgttcctcc agcggcgcga cggctacctg tggctgggga cccaggaggg gctggctcga
      721 ttcaacgggc ttcggttcac gcgaatcgcc atcgagcacc ccgccggcga gaggtttccg
      781 gacgttcgct cgctcctcga ggatcgcggc gcggacctct gggtcgggac gtacggctcc
      841 ggcgtcgccc gcctcaggga cggcacgtgg tcggtctact cgacgaagga cggtctgccg
      901 gccgactcgg tcaacgccct ccgtctcgac cgcgaagggc gaatctgggt cgggacgagc
      961 ggcggcctcg cccggatcga gctcgggcgg gacggcgagc ccagaatctc ctccgtcccg
     1021 gagctccggg gaatcgcggt caacgcgatc tgcgaggtgg agcgtggagt cctctggatc
     1081 gggacggccc acgccggcgt cctcgagctt cgcgcggggg ccctccggga catctcgccg
     1141 tctctgccgc tccgtggtcg caacgtcacc gcgatcctgg ccgaccgcga gggtgggcgc
     1201 tggctcggga tgtcaccgct cggcctggtc cgcgtcgccg gagcctcgcc tgaccagtcg
     1261 tgggacgtcg aggaggtcca gtggctcctt caggaccgcg acgggaacgt ctgggcggga
     1321 acgatcgcgg gcgtccttcg catcttcgag ggacgcgtct tcacgctgac gacgcgcgaa
     1381 gggctcgacg acgacctttc cctgacggga tacgaggacc gggacgggag catctggatc
     1441 gggacgtcga ccgtgggggt gcaccggctt cgagacagca ggttcacccc gatcacggag
     1501 aaggagggcc tctcggcgcg gttcgtccgg gcgctccttc cctcctccga cggcgccctc
     1561 tggatcggaa cctacggcgg cggagcgtgc cgggagaagg acggacgcat cgactgcttc
     1621 gacacctcgg caggactctc caacaactac gtgcggtcga tcgcccagac ccgcgacggc
     1681 gcgatctggg tcggcacgct gaacgggctg aacaggatcg cgggtggatc gatcacgcgt
     1741 ttcggcctcg cggacggcct cctcgccccc ttcgtccgat gcgtcttcgt cgaccgccgg
     1801 ggagacctct ggatcggcct cgattccggc gacgtgcacg tctggaatgg gggcgacccg
     1861 tcacgtgccc ggacccatcg gggcgtcgcc gacaagggca tcgtgacgat gcgggaggac
     1921 cccgacggcc ggctctggat cggaacctac ggcgacgggc tccacgtccg ggagaacggg
     1981 cagttccgtc gcgtcacggc gacgaacggc accctgcccc cctacgtcta ctgcatctgg
     2041 gagaaggacg gggagctgct tctcgggacg aacgccggcc tcgtgagcct gaccgggact
     2101 cgcatcggac cggccgcgaa ggacgaagcc tatacgggaa acatctacgg catcgtggac
     2161 gacggcgtcg gaagcctctg gttcacgtcg aaccagggtg tcttccgcgt ccccgagagc
     2221 gccatcgcgg agctgaggag cggaagcgcg ctccgggacg tcggcgtcca gtacgacgag
     2281 gccgacggca tgcccggagc ggagtgcaac ggcggtttcc cggccgcctg tcggcggccc
     2341 gacgggacgg tcctcttccc gacgacgcga ggagtcgccg cctaccgccc cgacacgaaa
     2401 ggattccgca ggcccccgcc gccggtcgtg atcgagacgg tcgatctgga cgaagagcgg
     2461 atcgagatcg cccgagcggg atcggtccgg ctcccgtcgg ggaagcgtcg cctgcgcttc
     2521 ggcttcgtcg ccctggacta cctcgagcct cggaagaccc gctaccgata ccgcctcgag
     2581 ggtttcgacg agagatggca cccgacgaac caggaccccg aggcgatcta cacgaacctc
     2641 cctcccggcg cctacacgtt ccgggtggtc gccagcgacg gcgagggcgg gtggaacccg
     2701 gccggggcgt ccttcgcctt cgaggttctg cctcgtttca cgcagacgat tccgttctac
     2761 gccctctgtg tcctcggagt ggtcgcgtcc gcggtgggag gccatcggct gcgcgtccgt
     2821 cggatgaagg tccgggagcg ggagctgacc cgcctcgtcg acgagcgcac gcgagacctg
     2881 gaaagggcga atctggagct ggcgcagctc tccctgacgg acaggctcac ggagatcgcc
     2941 aatcgccggg cactggagca gttcctggat cgggagtggg gccgcgccgt ccggaacggc
     3001 ctctcgttct cgcttctcat ggtggacgtg gactgcttca aggccttcaa cgaccggtac
     3061 ggccatccgg ccggcgacga gtgcctgcgc ggtgtcgccc gcgcgctgag gtcgagcgcc
     3121 cggaggccgg gggacctcgc ggcccgctgg ggcggggagg agttcgtcgt cgtcctctcg
     3181 gagacggacg cggaaggggc cgtcgtcgtc gcggagagga ttcgggcggc ggtggaagac
     3241 ctgcagatcg cccacgaggc gtcgccggtc tcgtcacacg tgacgatcag cgtcgggtgc
     3301 gcgaccggga ttccgggtcg gggtcgcgag tggacctcgc tcgtccgcgc ggccgacgag
     3361 gcgttgtatc gatcgaaggg gaacggacgc aaccgcgtcg aggcgggccg cctcgattcc
     3421 ccggacgacc cgaccggaga cgtgtcggcg cccggcccgg cccgcgctcc ggccgcatcg
     3481 gaagggtccg gcacgatcgg gtgaacgctc gggcgcccga gaccgagcgg gcaccggacg
     3541 gtcgcggccc ggccctttcg gccgacaggc gccgcccttc tcagaccgcg gtccgcctca
     3601 ggcgcaggaa gacgaaggcg ctgtagcccg cgacgagcgt gagggcgacg agcaggacga
     3661 cgagcagcga ggtgagcgcg tccgggctgg ccgcggcgtg cgcgatcgtc aggctctccc
     3721 cggcgccgcc ccgcgccgga acgagcgcgg gatagagccc ctgcccgacg atcccccaga
     3781 gggcggcgat cgagagcgag gaggacccga gcgccagggc cggccgcccc gccttcacga
     3841 agtagggaag cgcgacgagg gacgcgagca tgacgacggg ggcgatccaa gtgagcgggt
     3901 ggtggtacgg ctcccagagg tgcggcgcgg cgaagagcga gaagacgccg gtcaggccgt
     3961 ggagcgcgac cgccgtcacc caggcgcgca ggacgaggtc ctcgccctcg cccacgcgga
     4021 ggttcagcca gcaggccccc tggagtgcga agagggcgac ggtctcgacg gcgacgacga
     4081 gcgcgaacgg cgacaggagg tctccgaacg cgcccgcgaa gcgccccgcc tcgtcgagcg
     4141 gaaggccgag gaggacgttc cccagcatca gtccgagaag gaccgcggcc agggcgctca
     4201 gcgctccgaa cgcgacgtcg cggggacgat cgacggcccc ggaggcgcgt cccttgccga
     4261 gcaccacggc ccgcaggacg aaggcgagcg cgagggcgcc gaggagcgcc ccgaacgccg
     4321 acgccaccgc ctcgcggacg ggcgggaacg cggcgaagag agccgcgagc cccccgacga
     4381 gccagagctc gttcccgagc gtcgccgggc cgagcgtggc gagccgccgc cggcgcgttt
     4441 cgtcctcgcc cgaccgcgaa aggacgagcg tcccgacccc accgtcgatc ccgtcgagca
     4501 ccgagtaccc gaccaggagg acgccgacca ggaggaacca cgtcgtctgc aggtccgtca
     4561 tcggagcacc tccgccgggt cgtcgagtct ccgtcaggtt agcgccgcct ccgccgcgag
     4621 gacagaggaa accgcggcgc acgccgggcc ccgcgacgcg gcccatcgcc atgcgcctcg
     4681 acgagccctc gagcccggac gccgggacag ggacgccggc agagtgcaga gtgtagagtg
     4741 tagaccagac cccctccgcg agggggcttc agcggacgcg ccnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnagagtgta gagtgtagac cagaccccct ccgcgagggg
     4921 gcttcagcgg acgcgccaga tccccgtgcc ccgccccggc aggtagacgc ggatcgcccc
     4981 gggggcgccg ctcgggtcgg ccgcgcccgg atcggcgaag acgcgctcga acgagccccc
     5041 cggcacggcg aacgggccgg ccgactggaa ggagtccgac aggttgtgga cgacgaggac
     5101 ggtctccccc gcggacgcgc ggaggaacgc gaggaagggc gtgacgccgg acctcgccga
     5161 gaggagggtg atctctccct tccgcagcgc ctccgaggag gtccgcgccc ggatgaggct
     5221 ccggtagcgg gagaggagcg aggaggggtc gtttcgctgg gcggcgacgt tcgccgtctc
     5281 gcggcccggc gcgaacgcga accagggagt cccggtcgtg aaaccgccgc cgggcgtcgc
     5341 gtcccacggc atcggcgtcc gcttcgactc gtcgcccccg gtcggcccgt tctcgaggcc
     5401 cacctcctct ccgtagtaga ggaacggcgc gcccggaagc gtcaggagga tcgccgcggc
     5461 gttccggagg cgggccgcgt tccggccgag ctgcgtcgcg aggcgcacct ggtcgtggtt
     5521 cgtcagaaac ggggcgtcga tcgcgccggc cgggtagacc cgcgccacct cttcgagctt
     5581 cgcggcgacc ggagcggcgt tcccggaggc gaccgccttg aggaccaggt cggagagcgg
     5641 gaagttgaag ctcgccggga gctcgtcccc gcccgggact tccttcgagc cgtagtacgt
     5701 cgcgatcacc gcggtgtcgg cccagttctc gccgacgacg aacgcctgcg gcttcacccc
     5761 ccggacgtgc gccgcgaacg ccttcagcgc cgcgtgcgtc tcgggcgtgt cggcctggcc
     5821 gcctccggcc ccggtctcga cgaggtagcg cgtcgcgtcg aggcggaatc cgtcgacgcc
     5881 ccgctcgagc cagagcgccg agatccgccg catctctccg cggaagctcg cgttcctcca
     5941 gttcaggtcg ggcatcccgc cccagaagac gccgtagtag aaggcatcgc cgctccggtg
     6001 ccaggtcccg gcgttgccgc cccacggctg cttccagccc gggtccgtcg cgctccagac
     6061 gtaccagtcg cggtagggcg aggccggcga ggaggcggcg gagacgaacc acgggtgctg
     6121 cgagctgctg tggttgacga cgaggtcgac gatgaccttc atcccgcgcc ggtgcgcctc
     6181 gtcgaggagc ctcaggaagt cagcgttcgt gccatagtcc ggctcgatct tcaggtagtc
     6241 ggtggtgtcg tagccgtggt agctcgggga ctcgaagacc ggcatgagcc agagggcgtc
     6301 gacgccgagg tccgtcgtcg tcgccgggtc gccgtcgttg aggtcgtcga ggcgcgagat
     6361 caggcccggc aggtcgccga tgccgtcgcc gtcggagtcc tggaagctcc ggacgaagac
     6421 ctcgtagaag acggcgccgc gggcccactc gtggtcccag cccgcggagg ccgcgcgcga
     6481 gggggccggg acgacctccc tgtcggacgg cgagctgccg caggtgaggg cggcgagcgc
     6541 catcgggacg acgagcgcga gtcgcgtcga acgcatgggg acctccgggc cgtggcccgc
     6601 gggaaggata tcggaggggc gaggccgtga gcggcttcga cgcggcgcgc ctcggcgccc
     6661 tcgtgcacga cctcctcgcc gtcgtgaggc ggcgcccggc cgacctcctc ccgttcgagg
     6721 aggtccgcga ccgcctcaag ctccgccggg tcgtcgaccg cgggacgtcc gacgtgccgc
     6781 tcgagaagat cgtcgggacg ttcggacggc gcggcgagtt caaccgggtc ttcctgccgc
     6841 gatccgagtc gctccgggag cggtgggagg acgtgaagga cctcgccgaa ggggccgcgg
     6901 ggttccctcc cgtcgagctc tacctcgtcg gggacgccta cttcgccgtc gacggccacc
     6961 accgcgtctc cgtggcccgc tcgctcggcg cgcggacgat cgaggcgcac gtcctcgagt
     7021 tcctcactcc cgtcccgctc ggccccgacg cctcgctcga ggacgtcctc cggaaggagg
     7081 ggctcgccga cttcctcgag gcgaccggcc tcgtcccgtc ctctcccgac gagttccggg
     7141 tgacgcaccc gggcgactac gcggaaatgc tcgagcacgt cgcggtccat cgctatttcc
     7201 tcggcctgga gctctcccgt tgcacgtcct gggaggaggc ggtccgctcc tggtacgaga
     7261 cgttcttccg gccgatgacc gagacgatcc gcgcgagcgg cgtcctcgcg gacttccccg
     7321 ggaggaccga ggcggacctc tacctcttcg tcgcccgcca cctccacgcg ctccgggagc
     7381 gctgggggga cggcatcgcg ccggcccggg ccgtgaagca cctccgcctc cagacgaagc
     7441 ggaacgggtc gcggaaggcg aagggctgag gcgcctcagc ccccgttccc gccgttcccc
     7501 gccccgcccg ggcggaagcg ccccgtcagc gtcgcgagct cgcggagcca ggcggcgcgc
     7561 tccccgttga agtcccactt ccgggcgcgc caggcccagt tgtcggcccc cttccccggc
     7621 gtgttcatcc gcgcctcgct cccgaggccg aagacgtcct gcaccggaag gacggcgagg
     7681 cgcgcgacgg aggcgtaggc cgccctcacg aagatcccga tcgggtcggt cccgtcgcct
     7741 ccgagatagg cgcggacgcg gtgccgcgtc gcgtcgtccg ccttctccca ccagccgcgg
     7801 gtcgtgtcgt tgtcgtgcgt cccggtgtag acgacgccgt tcgggacgtg gttgtgcggc
     7861 tggtagtcct cgtcgtctcc gccgaacgcg aattggagga tcttcatgcc ggggaggtcg
     7921 agcgcggcca cgagagcctt cacgtcgggc gtgatctcgc cgaggtcctc cgccacgagg
     7981 tcgagctcgc cgagctcggc ggcgagcgcg tcgaagagct tccggcccgg tccctcctgc
     8041 caggagcccg cgatcgccgt ctcggcgccc gccggaaccg cccagtagcc ggcgaagccg
     8101 cggaagtgat cgaggcggag gagctcggtg agccggaggt tcgccctcat ccgctcgacc
     8161 caccagcggt agcggtcctt ctcgaggaca tcccaccggt agagcgggtt cccccagagc
     8221 tggccggtct cgctgaagta gtcgggcggg acgcccgcga cgcggacggg ctccccctcc
     8281 gcgtcgagct cgaagatctc ggggtgagcc cagacgtcgg cgctgtcgcc ggagacgtag
     8341 atcgggacgt cgcccaggat ccggatcccg cgtccgtgcg cctctccctt cacccgctcc
     8401 cactgccgga agaagaggaa ctggaggaag gcgtgccagg aggtctcctc ggcgagctcc
     8461 ctctcggcgc gcgcgagcgc cgcgggcctc cgaagccgga gctcctccgg ccactcgcgg
     8521 aaggcggccc cgccgtagcg tcccttcagc gccatgaaga gcgcccagtc gcggagccag
     8581 aagccgtggg ccgacgcctc ggcgaatccc tggagcccgg cccgcagcgc ggacggggcg
     8641 tcggtcttcc atcgccgcca ggcctcgcgg aggagccctt ccttccaggg gatcacgcgg
     8701 tcgaagtcga cgtggtgctc ggggaagccg ggggctccgt cgagagccga ggcggggagg
     8761 aggccgtcgg cgacgagaag ctcgggagag aggagcatcg ggctcccggc gaaggccgag
     8821 agcgtcccgt acggcgagtt cccgaacccg gtcggaccaa gaggcaggac ctgccagacc
     8881 gactggccgg cggaggccgc ccagtcgagg aaggcgtcgc actccggtcc gaggtcgccg
     8941 atcccgaacc ggcccggcag ggacgtcggg tggaggagga ggcccgcggc gcgcgttctc
     9001 atccggacga tcctatccgg agccggcaac gctccggggc ctgcctccgt aaaagtgggc
     9061 ggggaatgaa acggggacga ctctccctcc tcgcgcccgc ggcggttctc ctgctggctg
     9121 ccgcgggcgt tttttcgggc tgccgcggcg cccgggccgg cgatggtccc ggcacggcgc
     9181 ctccgaccgc cggctccggg accctgctcc tgaccggcga gctcgccgcg gtccggtcgg
     9241 ccgacctctt cgtccccgag acgccgctct gggagctcca gctccgcttc ctcgaggagg
     9301 acggcgcgct cgtgaagacg ggcgaccggg tcgccgagtt cgacgcgggg aacctcgcct
     9361 ccgagctcga gcagaagcgg atcgccgcga tcgaggcgga gaccgagctc gtccgtttcg
     9421 acgcggaccg ggaggtggcg gagctcgggc gcgagcaggc cgtccgcgcc cggacgaccg
     9481 acctcgagaa ggcccgcctc gacgccgacc tccccgccga gctccgctcg cggcgagacc
     9541 acgaggagaa gcagctcgcg ctccgccgcg cgcggacgga gctcgcgaag gccggggagg
     9601 agctcgccgg ctcaagcgcg gccgcggatg ccgatcgcgc cacgaagctc atcgcgctgg
     9661 agcgggcccg cgccgagcgc gagaagctgg aggcggcggt cgccgccctc acgctccacg
     9721 cgcccaagga cggcgtcttc gtcgtcgccg agaacccgcg cgagcggaag aagttcctga
     9781 cgggcgacac gctctggccc ggcctcgcgc tcgcgcggat tcccgacccc ggggcgcttc
     9841 gggtcgacgc gctcctcttc gacgtcgacg acggccgggt ccgcgcgggc gacgccgccg
     9901 tcgtcgtccc cgacaccttc cccgaccgcc gtctcccggc ccgggtcgcc tccgtcgcgc
     9961 cggtcgcgca gccgatcgga cgcggctcgc cgcgtatggc ctttcgcgtc caggtcgacg
    10021 tcaccggcgg ggagctcgac ggcctccggc ccggcatgtc cgtccgcgtc gagacgccgc
    10081 cggcgccgac gcggtccgtc gcggccgacc cgagccgcgt cccgctcccg cctcccgacc
    10141 ccgcgagcgc gcgccaggcg aaggccgaga tgcgcgacct cgtcctctcc gtcgagctga
    10201 agggcgagct ggcggcgctc gacggcgagc cgatcggccc cacgcgcctc cccgaggtct
    10261 gggactaccg gatctcgttc ctcgccgccg aaggctcgga ggtgaagaag ggccagcccg
    10321 tcctccgctt cgacacgacc gcgctctccc agcagctcga ggagaagcgg gcggaggccg
    10381 agtcggcggg caagcagatc gaccggcgcc gccgaacgct cgcgctcgcc cgcgccgacc
    10441 tcgagctgca gaaggcgcag gccgaggcgc gtcgcgcgaa ggccgcgctg aaggtcgacg
    10501 tcccgcccga gatctccggc gcgatcgagc tgacgaaggc gcgcctcgac ctcgcgctcg
    10561 cggaggcgga gtcggcctcc gtcacgcgcc gcctcgaggc ggccgagcgc gcggcccgcg
    10621 ccgagctctc gatcctcgag gggcgccggc gccgcgccgc gctccgggcc ggagagctga
    10681 cgacggcgat cgggaagatg acggtgcccg ctccgcgcgc cgggactgtc ctcctcatcc
    10741 cgagctggaa cggcgacaag aagaaggtcg gcgaccgctg ctggtacggc gagaagatcc
    10801 tcgaggtccc cgacctcgcc cgcctcgggg tgcagggcga ggtggacgag gccgacgccg
    10861 gtctcgtccg cgaaggggcg gccgtctcgc tccgcctcga cgcccacccc gactccgagg
    10921 tccggggaaa ggtgcttcgc atccagagga cggtccagcg cgcgtcgccc tccgttccgc
    10981 tcaaggtcgc tcgcctctcc gtcgtgctcg aggaggtcga cccgaagaag atgaagccgg
    11041 ggatgcgggt ccgcggccgc gtcgagaccg ggcgcgtcgc gcgcgccctc gccgtcccgg
    11101 ccgacgcggt cgtcccgacc gaggccggtc ccttcgtctg ggtccctcgt gggagcggcg
    11161 cccggccggt cgccgtgaag gtcggacggc gcaacggcga gtgggtcgag gtcctctccg
    11221 gcctcaccga agggaacgcc gtcctgcgcg tcgcgcgccc ggccccgaag ggagccgcct
    11281 cgtgagacgg cgcgccctcg tcgccctcgc cgcggcgctt ctcgccgccg ccgccctcgg
    11341 ggcgcgactc ctccggcctc ggacggcgga cgcccgcgtc ccgacgttcg tcgtgaaggc
    11401 ggcgaagctc gagcggacgg ttcgcgccga gggggtcctc cgcgccgtcg acgcgacgcc
    11461 cgtccgcgtc ccgggcaacg acgtgagcct gaagatcggc tggatgatcg aggacgggac
    11521 gcccgtgaag gcgggcgacg tcgtcgtccg tctcgacccg acgcagacga cgaagctcct
    11581 cgccgacggc cgggccgagc gcgcgaccgc ggaccggcgc ggcgacaagg aacgcgccga
    11641 ggccggagcc ctcatcgcga acctccgccg cgacgccgac caggcccgcg acgagcggtc
    11701 gaccgccgag acgttccaga gccgcgaccc ggagctcttc tcccggcacg agatcatcga
    11761 ggcggacatc gacgtctccc tcgcgacgca gaaggagcgc cacgccgtcg actcgaaggg
    11821 ggagcgcgag gccctcgcgc ggacgggccg cgagctcgtc gccctggagg ggcgcacggc
    11881 gcagctgaag atcgacaagg ccgagcgcga gctcgccgc
//
LOCUS       DAOONL010000099        11841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6011_length_11841_cov_18.735449, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000099 DAOONL010000000
VERSION     DAOONL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_11841_cov_18.735449"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..3346
                     /locus_tag="PLP50_12945"
     CDS             518..3346
                     /locus_tag="PLP50_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA52497.1"
                     /translation="MKSPRTAARTVCYAWWTTILLIALVTSAAASAQLIVDDAVDTFA
                     VRRGRSEAAASDAPASVLRSSAITPESCTAVRCTVSVRDETQRVVLRDLESRGRILLE
                     PLPDAHRLFFQHATYDFGAASFDRAFSGVDEVAAPGSNGLFAISFKSYPEPAWLKELG
                     AEGLRPLESIQTMGYYFYGPKRIADTAAARFPYVHGVLEVPPGLKRVNLDAITPGDDG
                     GPAPTLVVVVTQRRDDALDVMRSASGRDPELAYDAGPLQAYAATLSREDARNLSAFPE
                     VVSIRRGGSGGPSDERANRIIAGTWQTPGTSWPPVTQNPRLPSNTSSPPYWTGFLSQL
                     AGLFASLSTQERTLSLGNQTVAFLDTGIDSGLRRSQQPYCPPYLRSGSGSPCTLVFTT
                     DTTEGFEDPETRADDRSYHGTLVSSLAAGFAGQESPGRDINSPPGVYGNYAFTQGVAQ
                     GARVAMCKLWRNRRNTGTPENPRCPTLETYREINDQIFSDESAAGIHRMLNYALVELS
                     RSDVLPDFPPAQQVTGPGARLFNHSWNTRSIDYDADAVLLDQTTRLLSRASFTFDGGV
                     NRTGAGAPGLHIVSAGNIGQYYPPAAPPPPPLTEWPSPSQVTAPATAKNVITVGATES
                     YNQEGYNPNCGDASLVYADNPRQVYWNSRIGFPNQRLKPDIVAPATASYGRTSAEWNA
                     CPAGCSLDLDGTDQYGWSIGTSFAAPVAAGAAAIASEWLRVWSDQPSPALVKASLIAT
                     AQTLTRLQRCGVGCTPCCDECGDMRPAPDKYQGWGGLTLDRLLRPATNYFFFDQGRVL
                     RSVDPTWTQSLAVVDRLQDVNIALVWTDRHGTEPNAALVNLVNDLDLEVSYVAGGQTH
                     RYQGNAYYTSIDSCARDGYSLLNPAMPFDHKNNVERINIRGGSLPMGVSSITVTVSAF
                     SLTGDGIFPGDSADFRQDFALAVENARQ"
     gene            3421..4329
                     /locus_tag="PLP50_12950"
     CDS             3421..4329
                     /locus_tag="PLP50_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52498.1"
                     /translation="MKHRKSLTGALFAAAVLVAPEAARAGEGSRLYVPWVDAHAGTFH
                     RRDGVLVSVNWMSDFTLFNGGAEPATWRRIAIYSDGQLVVPDGSSACMLETEQTVPPG
                     YGGYVGGCIATPPRNGLAMLVLDTSAALIANATVSLVHTPLNDCAGPGHAEIRQGRVP
                     LPAFKGLFPPGSTVTFGEIDLGNPSIGYCAPPNQRYARRVNVTMFNGGDASATFRLRV
                     KAGINRPEDFYEQTVVVGPKEIIQLNRLPLDLDEVASRTEGGSVAPAWVLLSADQPYV
                     AYVSSVFEDPEPGAMPMEVYPPQRVE"
     gene            4492..5409
                     /locus_tag="PLP50_12955"
     CDS             4492..5409
                     /locus_tag="PLP50_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52499.1"
                     /translation="MKRALILTLVLSTTTSLSAAEQRHLWVPLIQAELGLEVIPGANP
                     ESGRAFLTMWLGNISAFNASGVRASMAPVAIYGGFVMAPSGHDFRMTLAPGTGDQFWR
                     WPWSVFAGPENGPVSFAEFVAEPGIILNAGVEKVQFSSGCPLPDRSISETLVSQGRSA
                     IPVFDALFPAGASVICGEVNLGAPRQICGNPPGQRYPRRVNVTLFNAGDQPATFTVQA
                     IPLYTSAEPIFEQTVDVAAKDVIQLNDVAIPRAENGHMTVDYDTYVWLRISCDQPFLA
                     YASSVFEGGAPGSMPFEVFAPRLAVTGAP"
     gene            5544..6383
                     /locus_tag="PLP50_12960"
     CDS             5544..6383
                     /locus_tag="PLP50_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52500.1"
                     /translation="MALLFSSLSRAETIVLPNVTGFVLGAQNALWSTEIRITNPSETV
                     RELRVIDWLGVNGALRTLEIQPGETRIVSGWELIHGGHRGRAVRLDEAVFGAAVCEVD
                     DGLLVQSSIQTYTFPYPGGGIGPGGDVACRSWQGGYEIDDVFFNCNPGVGSIITHDRG
                     FFAPEETIKIPWLFASPGFRRQNLVILNPDGVASTVEISITSADGRVTKTNRVTVGPR
                     SHFQINDLFRRPEWEDYLNAIGNQQYEKPAARATIRATTRLYALGYVISWENNTVSVS
                     LPM"
     gene            6536..7426
                     /locus_tag="PLP50_12965"
     CDS             6536..7426
                     /locus_tag="PLP50_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52501.1"
                     /translation="MNTLGRTLLGLLLTAGTLAHGANVVYYVPSFDVFSEYLRYPDRP
                     TTEGWRFWTSRCSVFNVGQEAIDVSVLGLFGPEGPYEVVVPRPMTLGPLESGQLLTSN
                     DRDIVQFAVIRAPENAVVRAEVERRQLVSSGCGSGAGSGRSADLGEGRAPLPVFREPF
                     PAGATTIMGPVDLGSPRVVTDCVSGYQQHGSRRVNVTLFNAGGEEAVFLIRSFRMQGD
                     PEPVLEVPVTLGPGRVHQVNGLPIFAAIAESGAPWRPQELAWVTVEATQPFLVYTSTV
                     FIDPEPATLPFQVFPPTRIR"
     gene            7510..10449
                     /locus_tag="PLP50_12970"
     CDS             7510..10449
                     /locus_tag="PLP50_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52502.1"
                     /translation="MISLASLALASALAAPPERPHLTPWVVVRPEATAPPAAGPAPGS
                     LSANAPGLLVEVRAADVGGPATPERVRRLFAEARAAGRRAGLLVELPEVKVPENAREA
                     EAVTADSLVPGLGALLASAREADLFVLSLPDLSGEVRSRRWLLMKAAAIARSVGPTAR
                     IAVLFHQPAGGGLFPPGAQEILSEEAAAFVDLLGLDLDDASTTPEAVRQAADGLSFAR
                     PLLVTAPQLADAGALVDLAARFSTVDSPVVAAPLADASGAPLLDRLGALLSGDVSRDG
                     RSAAAKTPDGKALSVLRLAKGTDLGGVVLVTGADETGAPARGPFVLELDGPSYTTAEV
                     RELATGRSRSFDLPKSGSPRLSLTTANGPLALVLTAREKAPEEATRASTDVTATRGLT
                     VEEILARHQAWRAGRDARWTRFTAVNRTSYRFRLEGFPTSFELTFEGPFFYERGKGFD
                     WAWQTAYVNGVRWKGKKIPELPLLQPEKVSELPLELTFNEAYRYVLDGDDTIGGVPCW
                     VVAFEPSAGFKAKAIYSGKVYIGKADFSLRRVAAKQLNLTGEVQSVDETTDFAEVAAP
                     DGGPALLQPVLTRGQSILRTFSRTTTLERTTELLEVRLDPSDFVERRAASLAGDQVMV
                     RDTDKGVRYLEKGKDGERVVVDDAKTSRLFGLGGVFYDDSFDYPLPLLGLYYIDLDAG
                     KKKKQLQGFWGGPIFAGSYNDPNLFGTNLDLGLDAFANLIRGDDALFEDGEKVDATTV
                     KARSFAANLNVGYPISRHLKATATLGGVWRDYAGGEDADPSYRVPSDHFVTRLEGRLG
                     WDLSGWTAVAKYSLNLRSKWEPWGYDGNPEWDEGKDQFRLFSVAVTKTFELPKFQRID
                     SGLTYARASNADRFSKYQFGFFGGTSLRGFRSGSLRAEEAVNAKLAYGIVAGKLVRLQ
                     LAYDHAVVKDEAAGLDWVHFGGAGLSGQFPGPWSTLVQLEAGLPVVGRNRGQTGFVLY
                     LVFLKAF"
     gene            10495..11112
                     /locus_tag="PLP50_12975"
     CDS             10495..11112
                     /locus_tag="PLP50_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52503.1"
                     /translation="MKRRPLALLVPLALLAAAPSPAAEVARWPLPAAAVMSADAAVNA
                     PPDLKRQLVKHRTRLMEGVKAGLAASPARDAAARRAALASGARRIAQAIRDHRRFEDV
                     AFEVGGLVAEAALLCGPPGPEPFEGDLIYFASERSVFRGFTARPFDAPETLVAAPLRT
                     DNPRVAYDSGVTISTRLLAWIWNAAGGDASIALARPESKGPYPVR"
     gene            11109..>11841
                     /gene="purH"
                     /locus_tag="PLP50_12980"
     CDS             11109..>11841
                     /gene="purH"
                     /locus_tag="PLP50_12980"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041741.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPA52504.1"
                     /translation="MSTKRALLSVSDRGGLLDLANALLRHGFELVSTGGTGKVLSDAG
                     LPVTQVSDVTGFPEVFDGRVKTLHPALFGGILYDRSVGKHVEQAAANGVGPIDVVVVN
                     LYPFEETVAAVETTFAQGIEKIDVGGPSLLRAAAKNHAHVAVLCDPADYAAFAAELDA
                     NGGASTLGTRKRLATKVFRRTAAYDAAISTWLSARTEEAQFAERLPLSFELGGTLRYG
                     ENPHQAAAFYRDPAAPPSALAGFVLH"
BASE COUNT         1925 a         4180 c         3807 g         1929 t
ORIGIN      
        1 gtctgcaccc cggaacgggt ccattgcccg atcgggtgag ggtggagagg ccgagtagcc
       61 ttagccgcgc caagccgctg gctgtacccg gatctcgaca ccgcctcgag acggatcggc
      121 ttcatgccag gtcgcccccg cgcgcgcggc cgctggttcc agaaagcgct gatcgggctg
      181 cctcgccgcg acctcgcgga tagatggaga ctgtcatgtc atccacgttc atcgccacct
      241 caaacagctc tcgagatgtg gtccttgtcg acgccccagc tgccatcatc ggtcgccccg
      301 agccggctcg ccccgctgcc tcactcgcgg gagcacgccc gaatccggag gcacgctccg
      361 aaaggcggat gaacgcctgg gagaccggcg ttggatgctg gcgcccccgg gcacttccgc
      421 gtgtggcgct ctggaggaat ccccctcggg gcgcgttctc ctccgcggtg gcacctgcac
      481 gagggctcgc aagtcgcaac cagagaagga gaagctcatg aaatcgccac gtaccgcagc
      541 gcgtacggtc tgttacgcgt ggtggaccac gattctgctg atcgccctcg tcacttccgc
      601 agccgcgtcc gcgcagctga tcgtcgacga cgccgtggac acgttcgccg tccgccgcgg
      661 aaggagcgaa gccgccgcgt ccgacgcgcc agccagcgtc ttgcgctcgt cagcgatcac
      721 acccgagtcg tgcaccgccg tgcggtgtac cgtcagcgta cgagacgaaa cacagcgtgt
      781 cgtgctccga gacttggaat ctcgcgggcg catccttctc gaaccgctac cggacgccca
      841 ccggctgttc ttccagcatg ccacgtacga tttcggcgcg gcgtctttcg acagggcgtt
      901 ctccggcgtc gacgaggtgg ccgctcctgg gtcaaatggc ctcttcgcca tcagcttcaa
      961 gagctacccg gaacccgcct ggctcaaaga gctcggtgcc gaagggctcc gtccgctcga
     1021 gtcaatccag acgatgggct actacttcta cgggccgaaa cgtatcgccg acactgccgc
     1081 ggcacgattc ccgtacgtcc acggcgtgct ggaagtgccg cccggtctaa agcgagtgaa
     1141 cctcgacgcg atcacgccag gcgacgatgg aggcccggcg ccaacactcg tggtcgtcgt
     1201 cacccagaga cgcgacgatg cactcgacgt catgcgttcc gcttccggac gcgaccccga
     1261 actggcctat gacgcaggtc cccttcaggc atacgcggcg accctcagtc gggaggacgc
     1321 gagaaacctg agcgccttcc ccgaggtcgt cagtattcgt cgtggcggat ccggcggacc
     1381 gtcggacgag cgagcgaatc ggatcatcgc cggaacatgg cagactcctg gaacgtcctg
     1441 gccccccgtg acccagaatc cacgtctccc gtcgaacacc tcgagccctc cgtactggac
     1501 cggcttcctg tctcagctcg ctggtctctt cgcgagcctg agcacgcaag agcggacgct
     1561 gagcctgggt aaccagacag tcgccttcct cgataccggc atcgacagtg gtctcaggcg
     1621 aagccaacag ccctattgcc ctccgtacct ccgctccggg tccgggtcgc cctgcacgct
     1681 cgtctttacg accgatacaa ccgaaggctt cgaagatccc gagacgcgcg cagatgatcg
     1741 ctcctatcac ggtacgctcg taagctcgct cgcggccggg ttcgcgggcc aagagagccc
     1801 cggtcgggat atcaactcgc ctcccggtgt ctacggcaac tacgccttca cgcagggggt
     1861 tgctcaaggg gcacgcgtcg cgatgtgcaa gctctggcgt aatcgccgca acacgggtac
     1921 gcccgaaaac cccagatgcc ccacacttga gacatatcgc gagatcaatg accagatctt
     1981 tagcgacgag tctgcggcgg ggattcatag aatgctcaac tacgcgctcg tcgagctcag
     2041 caggagcgat gttctcccag actttccgcc agcacagcag gtgaccggcc ccggcgcgag
     2101 actcttcaac catagctgga atactcggag cattgactat gacgccgacg cggtgcttct
     2161 ggatcagacg acgcgtcttc tgtcgagggc ttcgttcacg ttcgacggtg gcgtcaaccg
     2221 taccggtgcg ggtgcgccgg gtctgcacat cgtctccgct gggaatatcg gccagtacta
     2281 ccctcctgcc gcgccgcctc cacctccatt gaccgagtgg cccagcccgt cccaagttac
     2341 cgcgcccgcc accgcgaaga acgtgatcac cgtaggcgcc acggagagct acaaccagga
     2401 aggttacaac ccgaattgcg gcgatgcgag tctggtgtac gccgacaatc cgaggcaggt
     2461 ctactggaac tcgagaatcg gcttccccaa ccagcgactc aaaccggaca tcgtcgcccc
     2521 agcgaccgcg agctacggac gcacttccgc cgaatggaac gcatgccctg ccggatgcag
     2581 tctggacctt gacggcacgg accagtacgg ttggtccatt gggacgagct tcgctgcgcc
     2641 ggtggcggca ggagcggcgg cgatcgcttc cgagtggctt cgtgtctggt cagaccagcc
     2701 gtcgcccgcg ctcgtcaagg catctctgat tgcaaccgcg cagacgctga ctcgtttgca
     2761 gcgatgtgga gtcggctgta cgccttgttg cgacgaatgc ggagacatgc gcccagcgcc
     2821 cgacaagtac cagggatggg gaggactgac gctggatcgt ctcttacgac ccgcgaccaa
     2881 ctacttcttt ttcgaccagg gaagagtcct ccgaagtgtc gaccccacat ggacacagtc
     2941 ccttgcggtt gtcgataggt tgcaagacgt caacatcgcc ctcgtctgga ccgatcgtca
     3001 cggcaccgaa ccgaacgcgg cgctggtgaa cctcgtgaac gaccttgatc tcgaggtgtc
     3061 ctacgtcgct gggggtcaga cccaccgcta tcaggggaat gcgtactaca cgtccatcga
     3121 ctcgtgcgcg agggacggtt actcgcttct gaatccagcg atgcccttcg accacaagaa
     3181 caacgtcgag aggatcaaca tccgtggggg cagcctccct atgggagtca gttccatcac
     3241 cgtgacagtc agcgcgttct ccctgacagg cgacggcatc ttccccggag acagcgcgga
     3301 cttccggcag gacttcgcgc tcgctgttga gaacgctcgg caataggaca aggagcagca
     3361 tgtctccacg gcaagggccc aggagacgcg cctttcgcag agatgaggag gaggaggacg
     3421 atgaagcacc gcaagagtct caccggagct ctgttcgcgg cagctgtgtt ggtcgcgcct
     3481 gaagccgctc gagccggcga agggtcacgg ctctacgtcc cgtgggtcga cgcccacgcc
     3541 ggcacgttcc accggcgcga cggagtcctc gtgagcgtca actggatgag cgacttcacc
     3601 ctcttcaacg gaggagctga gccggcgacc tggcggagga tcgccatata cagcgacggg
     3661 cagctcgtgg ttcccgatgg gagttcggcg tgcatgctgg agaccgaaca gacggtcccc
     3721 ccgggttatg gtgggtacgt cggaggttgt atcgccacac cgccgcggaa cggcctggcc
     3781 atgctcgttc tcgacacttc ggcggcgctc atcgccaacg cgacggtgag cctcgttcac
     3841 acgccgctca acgactgcgc gggcccgggc catgctgaga ttcgacaagg acgagtcccc
     3901 ttgccggcgt tcaagggtct gttcccaccg ggaagcactg tcactttcgg cgagatcgac
     3961 ctcggcaacc cgagcatcgg ctactgcgcc ccccccaacc agagatacgc tcgtcgcgtc
     4021 aatgtgacga tgttcaacgg cggcgacgcc tcggctactt tccggctccg ggtgaaggca
     4081 ggcatcaacc gaccggaaga tttctacgag cagaccgttg tcgtcgggcc gaaggagatc
     4141 atccagctca accggctccc gctcgacctg gacgaagtcg cgagccggac ggagggaggg
     4201 tccgttgcgc cggcatgggt actgctttcc gcagatcaac catacgtcgc atatgtgagc
     4261 tcggtctttg aggatccgga gccgggagcg atgccgatgg aggtctaccc gccgcagcga
     4321 gtcgagtgag atgcacatca ctcagaaccg agcccggtcg atctgggggt cgacccaagc
     4381 gcccgccccg cctcctcttg aaggccgaag tcggcctcga cgacggcttg gcctgatccc
     4441 tgacgacgcc gaacgacggc aggacttcgc catcgctgag gagaacgcgc attgaagaga
     4501 gcactcatcc tcaccctcgt cctctccacc actacgtcgc tctcggcggc tgaacagcgg
     4561 cacctctggg tcccgctcat ccaggccgag ctcgggctcg aggtaattcc cggtgcgaat
     4621 cccgagagtg gccgggcatt cctcacgatg tggctcggca acatctcggc cttcaacgcg
     4681 agcggtgttc gagcgtccat ggccccagta gccatctatg gcgggttcgt catggcaccc
     4741 agcggtcacg acttcaggat gaccttggcg ccgggaaccg gcgatcagtt ctggcggtgg
     4801 ccgtggtcgg tcttcgcagg cccggagaat ggccctgtca gtttcgccga gttcgtagcg
     4861 gagccaggga tcatcctgaa cgccggagtc gagaaggtcc agttcagctc ggggtgtccc
     4921 ctcccggaca ggagcatcag cgagaccctc gtctcccagg gccgctcggc gatcccggtg
     4981 ttcgacgccc tgttcccggc gggcgcatcg gtcatctgcg gcgaggtcaa cctcggagct
     5041 ccccgtcaga tctgcgggaa cccacctggt cagcgctatc cccggcgggt gaacgtcacg
     5101 ctcttcaacg ccggggacca gccggcgacc ttcaccgtcc aggcgatccc gctctacacg
     5161 agcgccgagc cgatcttcga gcagacggtc gacgtcgcgg ccaaggacgt catccagctg
     5221 aacgacgtgg cgatccctcg cgctgagaac gggcacatga cagtcgacta cgacacctac
     5281 gtctggcttc gaatcagctg cgatcagccc ttcctcgcat acgccagctc cgtcttcgag
     5341 ggcggggctc cgggttcgat gccgttcgag gtcttcgcgc cgagactggc tgtcacgggt
     5401 gccccgtgac cgtcggtact ccgatgaaag tcgccatgga caacacgccg tgcccggcct
     5461 cgacgggagc ggccccaacc aggattgggc tctatatgtt tacaacgcgt actagaacga
     5521 tctcgcgagc gtcagcgctt ctcgtggcgc tcctcttctc ctccctctca cgtgccgaga
     5581 ccatcgtcct cccgaatgtc accggcttcg tcctcggtgc gcagaacgcc ctctggagca
     5641 ccgagatacg gatcacgaac ccctccgaga ccgtaaggga actgcgggtc atcgattggc
     5701 tcggggtcaa tggcgccctc cgaacgctgg agatacaacc cggcgagacc aggatcgtca
     5761 gtggctggga gctgattcac ggaggccatc gtggtcgcgc ggttcgtctc gacgaggccg
     5821 tcttcggggc cgctgtctgc gaagtcgatg atggtctcct cgtacagtcg tccattcaga
     5881 cgtacacgtt tccatacccc ggaggcggca ttggaccagg gggcgatgtt gcgtgccgga
     5941 gctggcaggg tggctacgag atcgacgacg tcttcttcaa ctgtaacccg ggcgtgggct
     6001 cgatcatcac ccacgacaga gggttcttcg ctccggaaga gacgatcaaa atcccgtggc
     6061 tctttgcctc acctggcttc cgacggcaga atctcgtcat cctcaatccc gacggcgtcg
     6121 cctcgacggt cgagatttcg atcacgagcg ccgatggaag agtgacgaag acgaacagag
     6181 tcacagtcgg ccctcggtcg catttccaga tcaacgatct gttcaggaga ccggaatggg
     6241 aggactacct gaacgcgatc gggaaccagc aatacgagaa acctgccgcg cgagcgacga
     6301 tccgcgcgac gacgcgactc tatgccctcg gatacgtgat cagctgggag aacaacaccg
     6361 tctccgtcag tcttccgatg taggtcgcaa tccccccggc ccgccggccc tccccggacc
     6421 ggcgggccga tgccctcact gacaggcacg gccgcggagc cgggccggag tccgggcctg
     6481 aagcgtcgtc ggcgctggac ctcgcccacg ccatcgtcgc agggagagag cagaaatgaa
     6541 tacgctcggc cgtacgctcc tcggtcttct acttacggct gggacgctcg cccacggcgc
     6601 gaacgtcgtc tactacgttc cgtcgttcga tgtgttctcg gagtacctgc ggtacccgga
     6661 tcgtccgacg acggaaggct ggcgattctg gacgagccgc tgcagcgtct tcaatgtggg
     6721 ccaggaggcc attgacgtct ccgtgctcgg cctcttcggt ccggaggggc cgtacgaagt
     6781 ggtagtgccg agaccgatga cgctgggccc gctcgagagc ggacagctcc tgacgtcaaa
     6841 cgacagggac atcgttcagt tcgccgtgat ccgggcaccc gagaatgcgg tcgtccgcgc
     6901 agaggtcgaa cgacgccagt tggtgtcttc tggttgtggc tcgggagctg gttcgggacg
     6961 cagcgcggat ctcggcgagg gacgggctcc gcttcccgtc ttcagggagc cgtttccggc
     7021 gggggcgacg acgatcatgg gtccggtcga cctggggagc ccgcgcgtcg tcacggactg
     7081 cgtcagcggg taccagcagc acggtagcag gcgagtgaac gtgacactct tcaacgcggg
     7141 cggcgaggag gccgtcttcc tgatccggag ctttcgcatg cagggagacc ccgaacctgt
     7201 gctggaggtc cccgtgacgc tcggccccgg gagggtccac caggtcaacg ggctcccgat
     7261 cttcgctgcg atcgcagaga gcggcgcacc ctggaggcca caggagctcg catgggtgac
     7321 ggtggaggcc acccagccgt tcctcgtgta cacgagcacg gtcttcatcg atcccgagcc
     7381 ggcgacgctc ccgttccagg tctttccccc cacccgaatc cgatagtcgt cccgttccat
     7441 ttttgggacg cgtcccgacg tgacgctcgt tccggacgca cggcccggcg attgcgtata
     7501 actcgcgcca tgatcagcct tgcctcgctg gcgctcgcgt cggccctcgc cgctcctccc
     7561 gagcggcccc acctcacccc ctgggtcgtc gtccgcccgg aggcgacggc gccgcccgcc
     7621 gccggaccgg ctcccggaag cctctccgcg aacgcgccgg gcctcctcgt cgaggtccgg
     7681 gccgccgacg tgggcgggcc ggcgacgccg gaacgcgtcc gccgcctctt cgccgaggcg
     7741 cgggcggccg gccggcgggc cgggctcctc gtcgagctgc ccgaggtgaa ggtgcccgag
     7801 aacgcgcgcg aggcggaggc ggtgaccgcc gactccctcg tgccggggct cggggcgctc
     7861 ctcgcctcgg cccgggaggc ggacctcttc gtcctctccc tccccgacct ctcgggcgag
     7921 gtccgctcgc gccgctggct cctgatgaag gcggccgcga tcgcgcggtc ggtcggcccg
     7981 acggcccgga tcgccgtcct cttccaccag cccgccgggg gcggcctctt ccctcccggc
     8041 gcgcaggaga tcctttccga ggaagcggcg gcgttcgtcg acctcctcgg cctcgacctc
     8101 gacgacgcct cgacgacgcc ggaggcggtg aggcaggccg cggacggcct ctcgttcgcg
     8161 cgcccgctcc tcgtgacggc gccgcagctc gcggacgccg gagcgctcgt cgacctcgcc
     8221 gcccgcttct cgaccgtcga ttccccggtc gtcgccgcgc cgctcgcgga cgcctccggc
     8281 gccccgctcc tcgaccggct cggcgcgctc ctctcgggcg acgtcagccg ggacggccgg
     8341 agcgcggccg cgaagacccc ggacgggaag gcgctttcgg tcctgcgcct cgcgaagggg
     8401 accgacctcg gcggcgtcgt cctcgtgacg ggcgcggacg agaccggcgc ccccgcccgc
     8461 ggcccgttcg tcctcgagct ggacggcccc tcgtacacga cggccgaggt ccgcgagctc
     8521 gcgaccggcc gctcgcgctc cttcgacctg ccgaagtccg gatcgccccg cctgtcgctg
     8581 acgacggcaa acggcccgct ggcgctcgtt ctgacggccc gcgagaaggc gccggaggag
     8641 gcgacccgcg cctcgacgga cgtcaccgcg acccgcggcc tgacggtgga ggagatcctc
     8701 gcgcggcacc aggcgtggcg cgccgggcgc gacgcccgct ggacccgctt caccgccgtg
     8761 aaccggacct cgtaccgctt ccgcctcgag ggcttcccca cctccttcga gctgacgttc
     8821 gaaggaccgt tcttctacga acgcggcaag ggcttcgact gggcctggca gacggcctac
     8881 gtcaacggcg tgcgttggaa ggggaaaaag atccccgagc tgccgctgct gcagcccgag
     8941 aaggtctccg agctcccgct cgagctgacg ttcaacgagg cgtaccggta cgtcctcgac
     9001 ggcgacgaca caatcggcgg cgtcccctgc tgggtcgtcg cgttcgagcc gagcgccgga
     9061 ttcaaggcga aggcgatcta ctcgggtaag gtttacatcg gcaaggccga cttctcgctc
     9121 cggcgcgtcg ccgcgaagca gctgaacctg acgggcgagg tccagtcggt cgacgagacg
     9181 accgacttcg ccgaggtggc ggcgcccgac ggcgggcccg ccctccttca gcccgtcctg
     9241 actcgcggac agtcgatcct ccggacgttc tcgcggacga cgacgctcga gcggacgacg
     9301 gagctcctcg aggtccgcct tgacccgtcc gacttcgtgg agagacgggc cgcgtcgctc
     9361 gcgggcgatc aggtgatggt gcgagacacc gacaaggggg tccgctacct cgagaagggg
     9421 aaggacgggg agcgcgtcgt cgtcgacgac gcgaagacct cgcgcctctt cggcctcggc
     9481 ggagtcttct acgacgactc gttcgactat ccgctgccgc tcctcggcct ctactacatc
     9541 gacctcgacg ccggaaagaa gaagaagcag ctccagggct tctggggcgg cccgatcttc
     9601 gcgggctcgt acaacgaccc gaacctcttc gggacgaacc tcgacctcgg gctcgacgcc
     9661 ttcgcgaacc tgatccgggg cgacgacgcg ctcttcgaag acggggagaa ggtcgacgcg
     9721 acgacggtga aggcgcgctc gttcgccgcg aacctgaacg tcggctaccc gatctcgcgc
     9781 cacctgaagg cgacggcgac gctcggcggg gtctggcgcg actacgcggg cggcgaggac
     9841 gccgacccgt cgtaccgcgt cccctccgac cacttcgtca cccgcctcga agggcgcctc
     9901 ggctgggacc tctccggctg gacggccgtc gcgaagtact cgctgaacct ccgctcgaaa
     9961 tgggagccgt ggggctacga cgggaacccc gagtgggacg aggggaagga ccagttccgc
    10021 ctcttttcgg tcgcggtgac gaagacgttc gagctgccga agttccagcg aatcgactcg
    10081 gggctcacct acgcccgcgc ctcgaacgcc gaccgcttct cgaagtacca gttcggcttc
    10141 ttcggcggga cgtccctccg cggcttccgc tcggggagcc tccgcgccga ggaggcggtg
    10201 aacgcgaagc tcgcctacgg catcgtggcc gggaagctcg tgaggctcca gctcgcctac
    10261 gaccacgccg tcgtaaagga cgaggcggcg ggcctcgact gggtccactt cgggggcgcc
    10321 ggcctttcgg ggcagttccc cggcccctgg tcgacgctcg tccagctcga ggcggggctc
    10381 cccgtcgtcg ggcgcaaccg gggccagacc ggcttcgtcc tctacctcgt cttcctcaag
    10441 gccttctgac gggcgccgga gccgccccat gccgaacgta gaatccggcc gccgatgaag
    10501 agaaggccgc tcgccctcct cgtcccgctg gcgctcctcg cggccgcgcc gtcgcccgcc
    10561 gcggaggtcg cccggtggcc cctacccgcc gccgccgtca tgtccgcgga cgccgccgtg
    10621 aacgcgccgc ccgacctgaa acgccagctc gtgaagcacc ggacgcgcct gatggagggg
    10681 gtgaaggcgg gcctcgcggc ctcccccgcc cgcgacgccg ccgctcgccg cgccgcgctc
    10741 gcgtcgggag cgcgacggat cgcccaggcg atccgcgacc atcggcgctt cgaggacgtc
    10801 gccttcgagg tcggcggcct cgtcgcggag gcggcgctcc tctgcgggcc gcccggcccc
    10861 gagccgttcg agggcgacct catatatttc gcctccgagc gttcggtctt tcgcggcttc
    10921 accgcccgcc cgttcgacgc gccggagacg ctcgtcgccg cgccgctccg gaccgacaac
    10981 cctcgcgtgg cgtacgactc cggggtgacg atctcaacgc gcctcctcgc ctggatctgg
    11041 aacgcggccg gaggcgacgc ctcgatcgcc ctcgcccgcc ccgagtcgaa aggaccgtac
    11101 cccgtccgat gagcacgaag cgcgccctcc tctccgtctc cgaccgcggc ggcctcctcg
    11161 acctggcgaa cgccctcctc cggcacggct tcgagctcgt ttcgaccggc ggcaccggga
    11221 aggtcctctc cgacgccggc ctccccgtga cccaggtctc cgacgtgacc ggcttccccg
    11281 aggtcttcga cggccgcgtg aagacgctcc accccgccct gttcggcggc atcctctacg
    11341 accgttcggt cgggaagcac gtggagcagg cggcggcgaa cggcgtcggg ccgatcgacg
    11401 tcgtcgtcgt gaacctctac ccgttcgagg agacggtcgc ggccgtcgag acgacgttcg
    11461 cgcaggggat cgagaagatc gacgtcggcg ggccgtcgct cctgcgggcc gccgcgaaga
    11521 accacgcgca cgtggccgtc ctctgcgacc cggccgacta cgccgcgttc gccgccgagc
    11581 tcgacgccaa cggcggcgcc tcgacgctcg gcacgaggaa gaggctcgcc acgaaggtct
    11641 tccgccggac cgccgcctac gacgccgcga tctcgacctg gctctcggcg cgaacggagg
    11701 aggcgcagtt cgccgagcgc ctgccgctct cgttcgagct gggcgggacg ctccgctacg
    11761 gcgagaaccc gcaccaggcg gccgcgttct accgcgaccc ggcggcgccg ccgtcggccc
    11821 tcgccggctt cgtcctccac c
//
LOCUS       DAOONL010000100        11732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6098_length_11732_cov_17.382376, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000100 DAOONL010000000
VERSION     DAOONL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6098_length_11732_cov_17.382376"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1148)
                     /locus_tag="PLP50_12985"
     CDS             complement(177..1148)
                     /locus_tag="PLP50_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPA52505.1"
                     /translation="MAFKDRPYFRRTLDLDDQLRSGKKVTATTLAARWETSTKTVQRL
                     VDGVRDEYGAPIEWDAHRGTYFYSDPTWHLPWLAMKGADLFAVGVAMKVLQMYEGTPV
                     AKDMRAIYERLSELMPAEVRISPSSFVEKLYIRPQAQRPVDPKVWDAVATGLREKVAI
                     EADYRKPAGGGGWRELEPLHLVLFGTDWFLLARDPEGEAVKTFYLSRMKAVRVGTRRF
                     SPPRGFRPEEHFGDTIGLYVGKPRFRFRVRFGREVAEWITEVRWHEKQKLVRRSDGDV
                     ELELPAGSLLEARRFVLSFGMHAEVLGPEELVEDVKAHVRSLAERYG"
     gene            1245..1379
                     /locus_tag="PLP50_12990"
     CDS             1245..1379
                     /locus_tag="PLP50_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52506.1"
                     /translation="MTTLLLVGLVLLLLGSFSESSSKAARRLRRLDPGGRDDFGGRLR
                     "
     gene            1430..3361
                     /locus_tag="PLP50_12995"
     CDS             1430..3361
                     /locus_tag="PLP50_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52507.1"
                     /translation="MKRARTAIEPFTSGEECLEAWFLALGPMTGTGGDHPTGIVPIDV
                     RLPHLRALAARSAATRDAGVPTPVLDAIEGLRLDLLDRLLLLALLRDDLDARTNGGLR
                     IATLCDAAGAATWSHQALVCSRLDETGALRRHGLVESDGDPAPGERLYRLALRWKEPL
                     LAGKTEAPPEDFEIPPTPCGRLQTAFSAAAMLLARVSPDPVERCTVWSYPVPDGPGWD
                     SAARVRRALLCSAHAYVSADGPASADPLALLLREAGATTVPEAALLVLLLCRGEDDGP
                     LAWGLLEPALGSPAASLPGPSSPLVAAGLVEVRPSTGSPQLSTCRATADARLRAVPSG
                     MPAPRAARAIGSEVPASPALVERIEPRLNLDGLVLPLATRRRLTEALAVPAALASLSE
                     TGWGVEETLLGAPNVALLLYGPPGTGKTLCAEAIAGQLGKTLWRLRTDQLLSKFVGET
                     EKRLTEVFAEARRTGDVVLLDEADSFLTTRERAARSWEATMTNVLLQEIERFRGVVVL
                     TTNRDAVLDPALERRLAARLAFPLPGAAERRLLWERHLPPRAPRAADVDLESIAERFA
                     LSGSAIRTAALYALARAASRAGDARVLTRADLEDAARVETARAASHRPAVGFGVATTP
                     SPRLALVAEHRDAARKERP"
     gene            3358..3834
                     /locus_tag="PLP50_13000"
     CDS             3358..3834
                     /locus_tag="PLP50_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPA52508.1"
                     /translation="MSDYPDLVDEPEETSAEVPAEEASLPPPPSDADPYFAFLAAEGF
                     RPEVDEDGDVRFRHEGRTLYLFRDGKGPEYFRVALPGAWECDGPEEESRALAAVNSVN
                     RDLKTVKCVLVDGVVWVSVEQFVDPPEAFRPVFTRLLDVIGSAAWQVRERMRASPT"
     gene            complement(4010..7429)
                     /locus_tag="PLP50_13005"
     CDS             complement(4010..7429)
                     /locus_tag="PLP50_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52509.1"
                     /translation="MKILAIRGKNLASLSGEFALDFETEPLKSSGLFAITGPTGAGKS
                     TLLDALCLALFDATPRLDTRGGPEIGRAGDAERLSANDVRSLMSRGATDAMAQVDFVG
                     RDDHRWRATWTVGRARGKKGGKVQKQNLSLVDLTVGQTAGGTKTETLAAIEDKLGLTF
                     DQFRKSVLLAQGDFADFLKANADERSLLLQRLTGAEIYERISVAAHRKAKEAKDRLRE
                     LEALRASVTVLSAEEREHLEAEAVAAAAEVEAHGAREKVLEELARQAKAAEESEEEAR
                     SRATTSLKAAGEAEAAGKRRESTQNEVVGATETLALATADLEARKPEIEKARELDVRV
                     SGAERALEEAKAAAAGASGKLEEARKALVDREGIERGLREEVGTLEAWLAGHSAAKEV
                     AARWEQADASLGELVRIADERAATTKRHDEAIRALETASRTAAAAGAAVDETRRQLTV
                     ATTARNEADAKLKGIPRAAHRERLDGLASRERNLAGLRDLFERATAAEKDRLQAVRNA
                     AGAEEKAKTARTEETAAAARLGELALLLPEAKRALSLSESALSLEEQRAGLVDGQPCP
                     LCGATEHPWAVAGAPVPATVELQRKRVRELETEERSAVAAKHAAAATAAAESEKAESE
                     RKRAAEREAQGAGLLDRWREGASAEALPDSPLEEGVAELLQSRADAVRDEKAAVKAAV
                     AEAEQIEAELGQHRKAAESLASQVEEAQAAKEKADAAVREAEKRHDAEQAALQAHDEA
                     AAREIRQLQPILAGRPELKETVATAPERVRAALSSEAAAYEAYSDLAEEKRVHLAAAE
                     KETASARSAVAPRAEQADAAQAEASSRETILVSLRTERLALLDGKSVAEVEEKLRKLL
                     AHSTWLLDEARKRDNDAATKHAAAKAQAEGAAARAAEVRTAADAAVERLRSGRAEVGL
                     PPETPIEDALAQARAALTEARERADARRAARLSDDEARTRQTGLEGEIAAHERSSKVW
                     LELDELLGSQDGKKFRIFVQSLTLEALVEAANEHLRDLARRYRLERVPGTDMDLQMID
                     SVLGDERRSVQSLSGGETFLVSLALALGLSSLATQRTRVETLFVDEGFGSLDADTLDK
                     ALSALEALQASGRRVGIISHVAALTERVGTRVAVTPRGAGRSAVSVVGAAG"
     gene            complement(7426..8682)
                     /locus_tag="PLP50_13010"
     CDS             complement(7426..8682)
                     /locus_tag="PLP50_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HPA52510.1"
                     /translation="MRIVHTADWHLGHVLYDRDREAEHRAFLDWLLDLLEKESVDALL
                     VCGDVFEGANPPASALALWYGFLADASRRCQGLQVVVVAGNHDSPARLEAPAPVLAAL
                     SVNVVGFLPRAEGEVDVERLVVPLRERSSGPGGEVAAWVAAVPFLRLGDLPGGEATPE
                     GIVDGVRAIYARALEAARSRRTAGQALLATGHCYVEGMRLSDESERKVLGGNQHPLPV
                     DVFPADVDYVALGHLHFPQQVGGRGNVRYSGSPLPLSLDEDVYPHQVCLVELQGGRCK
                     AVNAVRVPRSVPILRLPDEPAPLAEVLALLEKLPPAPPREAGEPDRRPWLEVRVRLEG
                     SEYGYQGEIAEALEGKDARLVKITPQRAGSGETLADGSERSLSDLTPEKVFRKLWERG
                     NADPVPAELEAAFHELVDAASQEEAS"
     gene            8849..8980
                     /locus_tag="PLP50_13015"
     CDS             8849..8980
                     /locus_tag="PLP50_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52511.1"
                     /translation="MTFLIILGVLFLLIGSFAESPSDSARRTRRLDPGGRDDFGGRF"
     gene            9120..11699
                     /locus_tag="PLP50_13020"
     CDS             9120..11699
                     /locus_tag="PLP50_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPA52512.1"
                     /translation="MRIVFDPDFDSGAWPGPLEGRAAVAGETWVGEAGLLGLLETSLG
                     LAGPPIPSSRRVAALVPAVRSSEGFWSASASIDPLAVSRRLLGWRDFLFFQGWRGEEI
                     PGAPRLTALAAVTHDALPGSVDRLLAVESALAVHSAEVAELSFLAEPYSFPTAWRRLF
                     DALARQGTRTAAMPLAPAPASGDLSAARGSGFVPTGDGSLQLVHPVGPLEAAESVAAW
                     LAAQDSLDGTVVISPEPVLDTALRRHGLPTTGAAATAADDALLPVVPLTLALGLDPAD
                     PQQALDLLLLPVGPVPKSVAGSLADALHDQPAVGSPKWTAALERALDRIEDESRRKAL
                     SERLEQLFRPGAIRESYPVEEARRRLAVLERWLRGRIERDDDPAPFERALSSCLDTAD
                     VLDRSGLTELTESQLGRVVAEASTALPASAPFPREAGLGVVGLPGGIGGPARRIVWWR
                     FQRAESLSSGSFPATPAERAALAAVGIEIETPGVESVRRALRWRRPLLYAGESLVLVA
                     PHRDAAGEELHPHSLWDEITASFAPGGRPDLLERPAPFGSRTPACSVRSLRPLPEPRV
                     DWSGPAVPPREVESPSSLEKLLGCSLLHALTYAGGLWPGRSGTLPGEAMLLGSVCHSV
                     LERVLARTGLTPDEAGEEAERLFDTTGPFLGASLFLPGAERDRAVARRAVRDAARHLV
                     GLAARLGVSRVEVERSLEAPWPGGTVRGRADLIFSSPSAVVDLKWSKGIRRDLLRDGG
                     ALQLAAYARLLAHEDGLIDPPPVAYFILRDALLLAQSESDFPGAEAVGGPPPREVWEA
                     LESAALAAREHVARGELASPGREQDEKAAAALTDEGLFVPAPCRYCDCGALCGRSFPA
                     EGA"
BASE COUNT         1354 a         4687 c         3940 g         1751 t
ORIGIN      
        1 accaggcccc ggagtacgag ccccccgcga tggcgcggaa gtagaagcct cctcccgcct
       61 cacctcaccc cacctcatct cacctcgagc cttcggacga cggcccggcg cgcgacgacg
      121 cgccgggccg tttcgtcctc ttcccggcgc cgacgccccg ggcgaggcgc ggcaccctac
      181 ccgtagcgtt ccgccagcga acggacgtgc gccttcacgt cttcgacgag ctcctccggc
      241 ccgaggacct cggcgtgcat cccgaacgag aggacgaagc gccgcgcctc gaggagcgac
      301 ccggccggca gctccagctc cacgtcgccg tcgctccggc ggacgagctt ctgcttctcg
      361 tgccagcgga cctccgtgat ccactcggcg acctcgcggc cgaagcggac ccggaagcgg
      421 aagcgcggct tgccgacgta gagcccgatc gtgtcgccga agtgctcctc cggccggaag
      481 cccctcggcg gcgagaagcg ccgcgtgccg acgcggaccg ccttcatccg ggagaggtag
      541 aaggtcttca ccgcctctcc ttcggggtcc cgcgcgagga ggaaccagtc ggtcccgaaa
      601 aggacgaggt ggagcggctc gagctcgcgc cagcctccgc cgccggccgg cttccggtag
      661 tcggcttcga tcgccacctt ctcccggagg ccggtcgcga ccgcgtccca gaccttcggg
      721 tcgaccggcc gctgggcctg cgggcggatg tagagcttct cgacgaacga ggagggcgag
      781 atccggacct cggcgggcat cagctccgag agccgctcgt agatcgcccg catgtccttc
      841 gcgacgggcg tcccctcgta catctgcagc accttcatcg ccacgccgac ggcgaagagg
      901 tccgccccct tcatcgcgag ccaggggagg tgccaggtcg ggtccgagta gaagtacgtc
      961 cccctgtgcg cgtcccactc gatcggagct ccgtactcgt cgcgcacgcc gtcgacgaga
     1021 cgctggaccg tcttcgtcga cgtctcccac ctcgcggcga gcgtcgtcgc ggtgaccttc
     1081 ttccccgagc ggagctggtc gtccaggtcc agggtccgtc ggaagtacgg gcggtccttg
     1141 aaggccatcg gctcctcccg ggaggagtat gcgcgatccg ccgatccatg gacaccgttt
     1201 gtctgaccct cgggccgaca ctgcgaggcg aaaggagacc ggccatgacc acgctcctcc
     1261 tcgtcggcct cgtgctcctc ctcctcggga gcttctccga gagcagctcg aaggcggccc
     1321 gccgcctgcg ccgcctcgac cccggcggcc gggacgactt cggcggccgg ctgcgctgac
     1381 cgatccgcga acgggcaccc gccgggaggg gtgccgtctg gaggcgacca tgaagcgcgc
     1441 gcgaacggcg atcgaaccct tcacgtccgg cgaggagtgc ctcgaagcct ggtttctggc
     1501 gctcggcccg atgaccggca cgggaggaga ccacccgacc gggatcgtcc cgatcgacgt
     1561 gcgccttccg caccttcgcg ctctcgccgc gcgcagcgcc gccacgcggg acgcgggtgt
     1621 cccgacgccc gtcctcgacg cgatcgaggg cctccgcctc gacctcctcg accggctgct
     1681 cctcctcgcg ctccttcgcg acgatctcga cgcccgcacg aacgggggcc tccgcatcgc
     1741 gacgctctgc gacgccgcgg gcgcggccac ctggtcccac caggcgctcg tctgctcgag
     1801 gctcgacgaa accggcgcgc tccggcgcca cggcctcgtc gagtccgatg gcgaccccgc
     1861 gcccggcgag cgcctctacc gcctcgcgct ccgctggaag gagccgctcc tcgccgggaa
     1921 gaccgaggcc ccgccggagg acttcgagat ccccccgacg ccctgcgggc gcctgcaaac
     1981 cgccttcagc gccgcggcca tgctcctcgc ccgggtctct ccggaccccg tcgagcgctg
     2041 cacggtctgg tcctatcccg tgcccgacgg gcccggctgg gactccgcgg cccgcgtccg
     2101 ccgggcgctc ctctgctcgg cccacgctta cgtctcggcc gacggacccg cgtcggccga
     2161 tcccctcgcc ctcctcctcc gcgaggccgg agccacgacc gtccccgagg cggcgctcct
     2221 cgttctcctc ctctgccggg gcgaggacga cggacccctc gcgtgggggc ttctcgagcc
     2281 ggccctcggg tcgcccgccg cttccctccc cggcccctcc tcccctctcg tcgctgcggg
     2341 cctcgtggag gtccggccgt cgaccggttc gccgcagctc tcgacctgcc gggccacggc
     2401 cgacgcccga ctgcgggccg tcccttccgg gatgccggcg ccgcgcgcgg cccgggccat
     2461 cgggtcggaa gtgcccgcga gccccgccct cgtcgaacgg atcgagccgc ggctgaacct
     2521 cgacggcctc gtcctgcccc tcgcgacgcg tcggcgtctc acggaggcgc tcgccgtccc
     2581 cgccgcgctc gcgtctctct ccgagaccgg ctggggcgtg gaggagacgc tcctcggggc
     2641 gccgaacgtc gccctcctcc tttacgggcc gcccggcacg gggaagacgc tctgcgccga
     2701 ggcgatcgcc ggccagctcg ggaagaccct ctggcgcctc cggaccgacc agctcctctc
     2761 caagttcgtc ggcgagaccg agaagcggct caccgaggtc ttcgccgagg cgcgccggac
     2821 gggagacgtc gtcctcctcg acgaggccga ctccttcctg acgacgcgcg agcgggccgc
     2881 gcgctcctgg gaggcgacga tgacgaacgt cctcctccag gagatcgagc gcttccgcgg
     2941 cgtcgtcgtc ctcacgacga accgcgacgc cgtcctcgac ccggcgctcg agcgccgcct
     3001 cgccgcgcgc ctcgccttcc cgcttcccgg cgccgccgag cggcggctcc tctgggaaag
     3061 gcacctcccg ccgcgcgctc cgcgcgccgc cgacgtcgac ctggagtcga tcgccgagcg
     3121 gttcgcgctc tcgggctcgg ccatccgcac ggcggccctc tacgccctcg cccgtgcggc
     3181 ctcgcgcgcg ggcgacgccc gcgtcctgac ccgggccgac ctcgaggacg ccgcccgcgt
     3241 cgagaccgcg cgcgccgcct cccaccgccc cgccgtcggc ttcggcgtcg cgacgacccc
     3301 gtcccccagg ctcgccctcg tcgcggagca ccgcgacgcc gcacgaaagg agagaccgtg
     3361 agcgactacc ccgacctcgt cgacgaaccc gaggagactt cggccgaggt gccggccgaa
     3421 gaggcttccc tgccgccgcc cccctccgac gccgatccgt acttcgcttt cctcgccgcc
     3481 gagggctttc gccccgaggt cgacgaagac ggcgacgtcc gcttccggca cgaagggcgc
     3541 acgctctacc tcttccgcga cgggaaaggc ccggagtact tccgcgtcgc ccttcccggc
     3601 gcctgggagt gcgacggccc cgaagaggag agccgcgcgc tcgccgcggt caactccgtc
     3661 aaccgcgacc tgaagaccgt caagtgcgtc ctcgtggacg gcgtcgtctg ggtcagcgtc
     3721 gagcagttcg tcgacccgcc cgaggcgttc cgtcccgtct tcacccgcct tctcgacgtc
     3781 atcgggtcgg cggcctggca ggtgagggaa cggatgaggg cgtccccgac ctgacggcga
     3841 ggctgcgccg cggcgatatc ggacccagga tggcgaggtc cacacacgct cgttcaccgg
     3901 cccgcccggc ctggccgtcc ggcgatcgcc gcaccggcga gctgaatgga atgaccgcat
     3961 gccggaggca gaagcgggcc tcctggaccg ggttcggccc ctcctcgagc taccccgccg
     4021 ctcccacgac gctcaccgcg ctcctccccg caccgcgcgg ggtgacggcg acgcgggtgc
     4081 cgacgcgctc ggtgagggcg gcgacgtggg agatgatgcc gacccttcgg ccggaggcct
     4141 ggagcgcctc gagggccgag agggccttgt cgagcgtgtc ggcgtcgagc gacccgaagc
     4201 cttcgtcgac gaagagggtc tccacccggg tcctctgcgt cgcgagggaa gagaggccga
     4261 gcgcgagggc gagcgagacg aggaacgtct cgcctcccga gagactctgg acgctccgcc
     4321 gctcgtctcc gaggaccgag tcgatcatct ggaggtccat gtcggtgccc gggactcgct
     4381 ccagccggta gcggcgcgcg aggtcgcgga ggtgttcgtt cgcggcctcc acgagcgcct
     4441 cgagggtcag gctctggacg aagatccgga acttcttgcc gtcctgcgag ccgaggagct
     4501 cgtccagctc gagccacacc ttgctgctcc gttcgtgggc cgcgatctcc ccttcgaggc
     4561 cggtctgccg cgtgcgcgct tcgtcgtcgc tcagccgcgc cgctctcctc gcgtcggcgc
     4621 gctcgcgggc ctccgtcagg gccgcccgcg cctgggcgag cgcgtcctcg atcggcgtct
     4681 ccgggggaag gccgacttcc gctcgtccgg agcgaagccg ttcgacggcg gcgtccgccg
     4741 cggttcgcac ctcggcggcg cgggccgcgg cgccctcggc ctgggccttc gcggcggcgt
     4801 gcttcgtggc cgcgtcgttg tccctctttc gcgcctcgtc gaggagccac gtgctgtggg
     4861 cgaggagctt ccgaagcttc tcctcgacct ccgcgacact cttcccgtcg aggagagcca
     4921 gcctctccgt ccggagcgag acgagaatcg tctccctcga ggaggcctcg gcctgcgcgg
     4981 cgtccgcctg ctccgcgcgt ggcgcgacgg cggaccgggc cgaggcggtc tccttctcgg
     5041 cagccgcgag gtggacgcgc ttctcctcgg cgaggtcgga atacgcctcg taggcggccg
     5101 cctcggagga gagagcggca cggacccgct cgggggcggt cgcgacggtc tccttcagct
     5161 ccggccgtcc cgcgaggatc ggctggagct gacggatctc gcgcgcggcg gcctcgtcat
     5221 gcgcctggag cgcggcctgc tctgcgtcgt gccttttctc ggcctcccgc accgcggcgt
     5281 cggctttctc cttcgcggcc tgtgcttcct cgacctgcga cgcgaggctc tcggccgcct
     5341 tgcggtgctg ccccagctcc gcctcgatct gctccgcctc cgcgaccgcg gccttcacgg
     5401 ccgccttctc gtcgcgcacg gcatcggccc gggactggag aagctctgcg actccttcct
     5461 cgagcgggct gtccggaaga gcctccgccg aggcgccctc acgccaacgg tcgaggagcc
     5521 ccgccccctg agcctcccgc tccgcggcac gcttcctctc ggactcggcc ttctccgact
     5581 cggcggcggc cgtcgcggcg gccgcgtgct tcgccgcgac cgcgctcctc tcctcggtct
     5641 ccagctcgcg cacgcgcttc cgctgaagct cgacggtggc cggcacgggc gcccccgcaa
     5701 ccgcccacgg gtgctccgtg gcgccgcaga gcgggcacgg ctgcccgtcc acgaggccgg
     5761 ccctctgctc ttccaggctc agggcggact cggagaggga gagcgcccgc ttcgcctccg
     5821 ggaggagcag cgccagctcg ccgagccgcg cggcggcggc cgtctcctcg gtccgcgctg
     5881 tcttcgcctt ctcctcggcg ccggccgcat tccgcaccgc ctggagccgg tccttctccg
     5941 cggcggtcgc ccgctcgaag aggtcccgca gcccggccag gttcctctcg cgcgaggcca
     6001 gcccgtcgag cctctcgcga tgggctgccc ggggaatccc cttcagcttc gcgtcggcct
     6061 cgttccgggc ggtcgtggcc acggtcagct gccgccgcgt ctcgtccacc gcggcgcccg
     6121 cggccgccgc agtccgactc gcggtctcca gggcgcggat ggcttcgtcg tgcctcttcg
     6181 tcgtcgcggc ccgctcgtcg gcgatccgca ccagctcgcc gaggctcgcg tcggcctgct
     6241 cccaccgggc ggccacctcc ttcgccgcgg agtgcccggc gagccacgcc tccagggtcc
     6301 ccacctcctc gcgcagcccg cgttcgatcc cctcgcggtc gaccagcgcc ttccgcgcct
     6361 cctcgagctt cccgctcgcc cccgcggccg cggccttcgc ctcctccagg gccctttcgg
     6421 ccccgctcac ccgcacgtcc agctcgcgcg ccttctcgat ctcgggcttt cgcgcctcga
     6481 ggtccgccgt cgccagcgcg agcgtttcgg tcgcgcccac gacttcgttc tgcgtcgact
     6541 ctcttctctt ccccgccgcc tccgcctcgc cggcggcctt cagcgaggtc gtcgcgcggc
     6601 tcctggcctc ctcctcgctc tcctcggccg ccttcgcctg ccgggccagc tcctcgagca
     6661 ccttctcccg cgccccgtgc gcctcgacct cggccgcggc cgcgacggcc tccgcttcga
     6721 ggtgctcccg ctcctcggcc gacaggaccg tgacgctcgc gcgcagcgcc tccagctcac
     6781 ggagccggtc cttcgcttcc ttcgccttcc ggtgggcggc gacggagatc cgctcgtaga
     6841 tctcggcgcc ggtgaggcgc tgaaggagga gcgaccgctc gtcggcgttc gccttcagga
     6901 agtcggcgaa gtcgccctgg gcgaggagga ccgacttccg gaactggtcg aaggtgaggc
     6961 cgagcttgtc ctcgatcgcg gcgagcgtct cggtcttcgt gccgccggcc gtctggccga
     7021 ccgtgaggtc gacgagcgag aggttctgct tctggacctt cccgcccttc ttcccgcgcg
     7081 cccggccgac cgtccacgtg gcccgccacc ggtggtcgtc ccggccgacg aagtcgacct
     7141 gggccatggc atccgtcgcc ccgcggctca tcagggagcg gacgtcgttc gccgagaggc
     7201 gctcggcgtc gcccgcccgt ccgatctcgg gcccgccccg ggtgtcgaga cgcggggtcg
     7261 cgtcgaagag ggcgaggcag agcgcgtcga ggagcgtgct ctttcccgcg cccgtcggcc
     7321 cggtgatggc gaagaggccg gagctcttca gcggctccgt ctcgaagtcg agcgcgaact
     7381 cgcccgagag gctcgcgagg ttcttcccgc ggatggcgag gatcttcacg acgcctcctc
     7441 ctgcgaggcc gcgtccacga gctcgtggaa ggccgcctcc agctcggcgg gcaccgggtc
     7501 ggcgttcccg cgctcccaca gcttccggaa gaccttctcc ggggtgaggt ccgagagcga
     7561 cctctcggag ccgtcggcca gggtctcgcc ggagcccgcc ctctgcggcg tgatcttcac
     7621 gaggcgcgcg tccttcccct ccagcgcctc ggcgatctcg ccctggtagc cgtactccga
     7681 cccctcgagc cggacccgca cctccagcca ggggcgccgg tcgggctcgc ccgcttcgcg
     7741 gggcggagcg ggcgggagct tctcgaggag cgcgaggacc tcggcgagcg gcgccggctc
     7801 gtcgggaagg cggaggatgg ggacgctccg cgggacgcgc acggcattca ccgccttgca
     7861 ccgcccgccc tgaagctcca cgaggcagac ctggtgcggg tagacgtcct cgtcgagcga
     7921 gagcggcagg ggcgagccgg aatagcggac gttgccgcgg ccgccgactt gctgggggaa
     7981 gtggaggtgg ccgagcgcga cgtagtcgac gtcggccgga aagacgtcga ccggcagcgg
     8041 gtgctggttc cctcccagga ccttccgctc gctctcgtcc gagaggcgca tcccctcgac
     8101 gtagcagtgc cccgtcgcga ggagcgcctg accggccgtc cgcctcgagc gggcggcctc
     8161 cagcgctctc gcgtaaatcg cgcggacgcc gtcgacgatg ccctcgggcg tcgcctcccc
     8221 tccgggcagg tcgcccagcc tcaggaacgg gacggccgcc acccaggcgg cgacctcgcc
     8281 gcccgggccg gaagagcgct cgcggagggg gacgacgagc cgctcgacgt cgacttcacc
     8341 ctcggcgcgc ggcaagaacc cgacgacgtt caccgaaaga gccgcgagga cgggagcggg
     8401 tgcctcgagc cgtgccggcg agtcgtggtt gcccgccacg acgacgacct gaagcccctg
     8461 gcaccggcgg ctcgcgtcgg ccaggaagcc gtaccagagg gccagcgccg aggcgggcgg
     8521 gttcgccccc tcgaagacgt cgccgcagac gaggagggcg tcgacgctct ccttctcgag
     8581 gaggtcgagc agccagtcga ggaacgcccg gtgctccgcc tcgcggtccc ggtcgtagag
     8641 gacgtgcccc aggtgccagt cggccgtgtg gacgatccgc atcactcctc cgtcggtcgg
     8701 gccgggactc ggttggcgcc gccctcttcg aactgtctca tgtctcgaag atcgcacgag
     8761 gggtcggaca gctggcgtcc gggcccaggg catcagacac cttctgtctg actccatgcg
     8821 cgacattgcc tggcgaaagg agccagccat gacgttcctg atcatcctgg gcgtcctctt
     8881 cctcctgatc ggcagcttcg cggagagccc ctcggattcc gccaggcgaa cgcgtcggct
     8941 cgacccgggt ggcagagacg actttggcgg acgtttctga cgttcgtgag ggacggccgc
     9001 tcgggcaggt cgaggccggt atcccggatt ggccggcctc gatctccaat gtctggcaac
     9061 atctcgcaac actccgcaga atctcgccgg gtgcggaacg ctcgccagtt gagggacgcg
     9121 tgagaatcgt cttcgatccc gacttcgact ccggagcctg gcccggcccc ctcgagggcc
     9181 gggctgccgt cgccggcgag acatgggtcg gggaggccgg gctcctcggc ctcctcgaga
     9241 cgtccctcgg tctcgcgggt ccgcccatcc cttcctcgcg gcgcgtcgcg gcgctcgtcc
     9301 cagccgtccg gagttccgaa ggcttctgga gcgcctccgc ctccatcgac cccctcgccg
     9361 tctcacgccg cctcctcggc tggcgggact ttctcttctt ccagggctgg cggggagaag
     9421 agatccccgg cgcccctcgt ctcacggcgc tcgccgccgt gacgcacgac gcgcttcccg
     9481 ggtccgtgga ccgcctgctc gccgtcgagt cggccctcgc cgtgcactcc gcggaggtcg
     9541 ccgagctgag cttcctcgcc gagccgtact ccttcccgac ggcctggcgg cggctcttcg
     9601 acgccctggc acgccagggc acgcgaactg cggcgatgcc cctcgctccc gcgcccgctt
     9661 cgggcgacct ctcggcggcg cgcgggtccg gcttcgtccc cacgggcgac ggctcgctcc
     9721 agctcgttca ccccgtcggt cctctggagg cggccgagtc cgtcgcggcc tggctcgcgg
     9781 cgcaggactc gctcgacggc accgtcgtca tctcccccga gcccgtcctc gacacggcgc
     9841 tccggcgcca cggcctcccg acgaccggcg ccgccgcgac cgccgccgac gacgccctcc
     9901 tccccgtcgt gcccctcacc ctcgctctcg ggctcgaccc ggccgacccg cagcaggcgc
     9961 tcgatctcct cctgcttccc gtcggtcccg ttccgaagag cgttgccggc tccctcgccg
    10021 acgcccttca cgaccagccc gccgtcggca gcccgaagtg gacggctgcc ctagaacgcg
    10081 ccctggatcg catcgaggac gagagccgac gaaaggccct ctccgagcgc ctcgagcagc
    10141 tcttccgccc cggggcgatc cgcgagtcct accccgtgga ggaggcgcgc cggcgcctcg
    10201 ccgtcctcga gcgctggctt cgcggccgca tcgagcggga cgacgacccc gcgccgttcg
    10261 agcgcgcact ctcctcttgc ctcgacaccg ccgacgtcct cgacaggagc ggcctcaccg
    10321 agctgaccga aagccagctc gggcgcgtgg tcgccgaggc ctccaccgcg cttccggcct
    10381 cggcgccctt cccccgcgag gcgggcctcg gcgtcgtcgg gctgcccgga ggcatcggcg
    10441 gccccgcgcg gcgcatcgtc tggtggcgct tccagcgggc ggagtcgctt tcctcgggca
    10501 gctttcccgc cacgcccgcc gagagggcgg cccttgcggc cgtcggcatc gagatcgaga
    10561 cccccggcgt cgagtccgtc cgccgcgctc ttcgctggcg acggccgctc ctctacgccg
    10621 gcgaatccct cgtcctcgtc gcgccccacc gcgatgccgc cggcgaggag ctgcacccgc
    10681 actccctctg ggacgagatc accgcatcgt ttgctcccgg cggtcggccg gacctcctcg
    10741 agcgccccgc cccgttcggc tctcgcacgc ccgcgtgttc cgtccgctct cttcggccgc
    10801 tccccgagcc ccgggtcgac tggagcgggc ccgccgtccc cccgcgcgag gtcgagtccc
    10861 cgtcttcgct cgagaagctc cttggctgct ctctcctcca cgcgctcacc tacgccggcg
    10921 ggctctggcc ggggcgctcg ggaactctcc cgggcgaagc gatgctcctc ggaagcgtct
    10981 gtcactccgt cctcgagcgc gttctcgccc gcacgggcct cacgcccgac gaggccggcg
    11041 aggaggccga gcgcctcttc gacacgaccg ggcccttcct cggcgcgtcg ctcttccttc
    11101 cgggagccga gcgcgaccgc gccgtggccc gccgcgccgt gcgcgacgcc gcccgtcacc
    11161 tcgtcgggct cgcggcccgc ctcggggtct cgagagtcga ggtggaacgc tcgctcgagg
    11221 ccccctggcc gggcggcacc gtgcgcggcc gggccgacct catcttcagc tcgccgtccg
    11281 ccgtcgtcga cctcaagtgg agcaagggga tccgccggga tctcctccga gatggaggcg
    11341 ctctccagct cgcggcttac gcccggctcc tcgcgcacga ggacgggctg attgatcctc
    11401 cgcccgtcgc ctacttcatc ctccgcgacg ccctccttct cgcgcagtcc gagagcgact
    11461 tcccgggcgc cgaggccgtg gggggcccgc cgcctcgcga ggtgtgggag gcactcgaat
    11521 cggccgccct cgccgcgcgg gagcacgtcg cccgcgggga gctcgcctcg cccggccgcg
    11581 agcaggacga gaaggccgcc gccgccctga cggacgaggg cctcttcgtc cccgcaccgt
    11641 gccgctactg cgattgcggc gcgctctgtg gccgaagctt cccggcggag ggagcatgaa
    11701 gcccgtccag gtcatctccg cgagcgccgg ca
//
LOCUS       DAOONL010000101        11565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6241_length_11565_cov_11.858123, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000101 DAOONL010000000
VERSION     DAOONL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6241_length_11565_cov_11.858123"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1372..2286
                     /locus_tag="PLP50_13025"
     CDS             1372..2286
                     /locus_tag="PLP50_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52513.1"
                     /translation="MEGGRGRPRLVREAEWPEYRGLYASTVRDLPNTPEYGRLKAWWK
                     RLTSATLAEDVLQILRDIEGLAAPNEFEPGFFYRMQWLPYVGLAAEAETRVNAALEAE
                     LGGLAFAKAREWTDRQGDEARVELALEVAWLRDGAVTGPWGGRRRVHEERRRLGLRQG
                     ARPLRRGRCPRVVADTKWKRLDPRKADLGVSGEDVYQVLAYAHRYATDTAVLIYPHHA
                     AVGRAGFQRDFVIQGAGAAPVVVRVITVDLARLESVAGQMGEAAGEAPAPYGSRGEAR
                     PRSLRGIVSTPTESPPLRRRRNGERPSA"
     gene            complement(2231..4882)
                     /locus_tag="PLP50_13030"
     CDS             complement(2231..4882)
                     /locus_tag="PLP50_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52514.1"
                     /translation="MSKRRWSVDVGFSRPAELPGIPSFQDALDALRAAAVNRHVPKGG
                     TPLSEADASRLRALAADAFTDGPIRALEEVDRLWTSGADRRLLLDLASLSYVTLLVQL
                     SDSIEMADLVAGRALGLLALSEVASGVHRDERAGVLSDAMGYSSPGMVRRDASWKRTF
                     PRPHIPGNERHDGPTGSAADTSPDARYLDLLEEARSLSAAQMQDRLGALGSSNGAPGV
                     HLLRRCRGGDLGEKSALGFALIEVAFTEAGIANVPRDSPVVPAFERSLASLPPADGPF
                     WDSGARNARLRALFYSGLADIGDFYLDELSSGPGATEFAQLLAGAESGLGAQFRQLYD
                     HLAAVKSGRMPVAKLVNDLDALPGLGQAALRRLGDALQSSLWATAPELPRVGAKLAHL
                     LDSRPANRLLFSRICHSALFDPVAMRAYYLSASTPGSTASATDVWLADTLGDIPRLTA
                     IASDPAVAAWPRLIAVEYLEDRSAIPDTELQRVLLDILASRPDTWSALRSVRLLSEHG
                     FLEDAERFLRLWLGSHPDAPALSKAVYASRLEHVLFLQGRFDEAWSTIEPHIPTWKGD
                     ALGAGAEALLSLGRTDEALKMGEACIERYPDDSMARTGLAKLLWRLGRHDAAAKPLLD
                     PRYPLSAEDWRVLVPREFLEVFRARPTEEAKPALEALLAAGASPWFLFEVASRFERAG
                     VPETSIVLLDAISRAKNEGIDGYLRAYRFRRRAHGAESADTWFRKNVVTSRSASWVGT
                     TAFDEREFDLLWLLPDDEKSWLLRAQAAAFEGRPEGSRLERLRAHFSDPAAPRLHAVD
                     GRFLLGEATDADLLATATDASRRCEVAYLLGLKAAGEKKLEDASAWLRAAVRTEQMKT
                     PAYNRAKEMLKRWDVRRFGVVGGAEIR"
     gene            5610..6089
                     /locus_tag="PLP50_13035"
     CDS             5610..6089
                     /locus_tag="PLP50_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52515.1"
                     /translation="MPSPKAQLKPAVGLASLPNIELEKTDVDGIVASATKSVASSMGI
                     RPEQLPSTVVDAIKVSAATGISTRTKALIQRNVDSSVKDAVLKGAKPLEMLDARVQAA
                     SDGIAHIVQDGKVDAVLADTAKLLWKKYAALKTAGFTDTQAFDLVLAEVQGRAGRNR"
     gene            6098..7717
                     /locus_tag="PLP50_13040"
     CDS             6098..7717
                     /locus_tag="PLP50_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52516.1"
                     /translation="MSWCAFVCDAVDGAAGWVEDQAEDAVGSVVDSVEDGLSAVVDYF
                     EGVGESVEEAVSDVAQLAADVAEAAWNGITEAASSVWDWIKRVAGKVWEWTSGALEDA
                     WNWAREAASDAWDWISGAAESVWGALCSAWDAVSGFVEEKVIPFLVDALWVLTHVDDV
                     LIAVSLGLQCLLTGQDEREYDFIEGLYLLDEAALAQRKVTFLPVQDDYVVFSDIHMFV
                     AGDPLDEFRQLGNSELYQAVLLFYCKGGYTLVENGDIEDLWMRETTVGEALLDQATDI
                     LGWPFGEMIEDEHEKYRIRSQAVKIFDNNSDVYQTIRALFHDQRKLVRLLGNHDDAWR
                     SDDYLPGLQEIYPELEVFDYALLGDYGGTPPAYGNHPRVIIAHGHQLDAWNNSICRAA
                     GAAMTECASGIPALATKVTERSEWEPTLLWKEGFPNELKQDIAGVDEVEFYETVEDDF
                     RSYSYVPQFILGHTHRPLEDPQIPEWKDCDERKFTEYTNDGTAGRWEQFIWCATVENG
                     SVSLYGWTWDAAGKPFARRFVGAPADCLRPV"
     gene            7986..9017
                     /locus_tag="PLP50_13045"
     CDS             7986..9017
                     /locus_tag="PLP50_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52517.1"
                     /translation="MPAVDSERSRVLILSFHAARAIPGKTVAGLFDEDVLFKKHASLR
                     GLLEQDAMDEHIAPESIVNDDEHGGVEKVEHDGASAWTLSAVTRRVWDRVGYSNPSTH
                     ESDFSMWFQSLWTQPIDCLVIAGHHGWAKEVGPFFWGSEQPKRGGDHRPYTSIDVHPE
                     DDAPAIGVSGYSYSSSRLRERIRPFLVMHALSRCSLLVIMGCNGVPAPADTLRMGTAW
                     QDWVRSANPQNERPIVLGWWAKHQMPRNTRKGENPEHFSEDFWTRMKGLAGGLQGPRP
                     LQTLCRTKPKAVIEAWAASMKGAFGASKTGQRHLWFETGKGVGAIDPDGAVWRVTRPE
                     GPIEKDKSS"
     gene            9014..11545
                     /locus_tag="PLP50_13050"
     CDS             9014..11545
                     /locus_tag="PLP50_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52518.1"
                     /translation="MKKVNVLTLDDVLELSGPSLNLGATEATGATRVVLREGGEEIEF
                     FTDASCDGGPAELDRRTSLTWDRGDVAVSKGRLTLKDKEEVELTFHWPNPLTPAGSGL
                     WLRLAIVRDGAGHWVRMAQQYPATGATVWWRRLTSRPAWEEYRLPLRVPRVSVAQLLS
                     VWKNQGFGYASGDPAAGSCPLVAGADGPACLVELDARERGRGVFLLAAEKRWASARFR
                     AEGVRARTGQRALGLSELGFAPRPYVFQGDLDLRSAGGPREFFLEWPGIPAGGPLPSE
                     VLSLQALWQEVAQHYALGLAATRARRHLTFVPTELQGRSDPVNLHIRVAADPRSAGGP
                     PLWKANLFQIGPVDCRLDLVLGGARTEEGCALRVPVWARSSEWKPIVDHLHDRFVKER
                     PTIPPLLGLGLDLDSASDRPVSSALVVSGVRFDVQGFERGRLRCTAEPGADVHGRPPL
                     GVDLRLDFTVSTPRAATQDPEIGFETQSSWLGRERPICVDLSEETRTTFDLCVVERAT
                     GGLSRLLRLTVRAPEGLGSLTTDTFVLDPSPLQVARVLTANKLEEGTVLAEYTDDSDL
                     PPEWAFRTETGQMDIVLPPQVIGEEMIKGKLAVGGKPVPDPNALFDFRLSAPAYLRLD
                     RTDVDAARASAPWALRRLLSQRLGVTGVRLQEAIFELLYGLTTTLRAPGLRIAELDAF
                     VGRVPFADELCALLGVEIEAPDATGELTVAELRRRHATDLARWIRALLHRPAQMPVFR
                     EWTDRRKLTLFEGVGFALRPSRQTAHPFDLTSFADRTPPDQHDKARRPLRGGVDFPFE
                     TRSIYEAFRRSTTPNDGTVSGLTFGSLGGTGGQQATPVQDRSVIA"
BASE COUNT         1888 a         3767 c         4127 g         1783 t
ORIGIN      
        1 tcgcgggtcg tggtgaggat cgcgacgctc gatttggcgc ggctcgagag ggtggcgggg
       61 gaggtggggg cggcggtcga gactagatga gcgcctgttc ggcggggggt tggagcgact
      121 gctggagagc cctctggcgt cacggcgtcg taagcgtccg atgaagcgcg tcaggggtgg
      181 tcgcggatcg accgccggct aaacgccgac ggtcaacccg agcccgtctc cgacagggtg
      241 agtcggacgc taacgtcgtg ccggcgtagc gaggtcagcc gctatcgtct atcggcgccc
      301 ctccggggtg ccgatcctgc attcgggctt gaccgagttg tcgacgtcct tctcgggcgg
      361 cggctccttc tttgaagaag gatcgggatc tcgctcggac atcggtagcg gcgctcgcgt
      421 ggcggcacca cttgagaacc ctctgccagg cacccggacc ttcctcgttc ccattggcgt
      481 tctcctctcg tctggtggtc gcggcagggg cgtctctcgc cactaccagg ggtcacgcgg
      541 agtcgggacc ttcggcttcg gccgcttggc ccgacgcggc ccgccgatga cggggtcggg
      601 ctccggaccc gggagtggga tcggttgagg atcatccggg tgcgggtcca gcgaaggcgg
      661 gtcggggtcg tgcggggggt cgtaccagcc ggggaggggc ggcgggcgct tcgcgggtct
      721 gccgctcggc actccgggag agtgagacgt cccagcagat ctcgcgctga gtccacctgt
      781 cgggccgtgg cgtggcaccc ctctccgcgt ggtcatcact cacctccggc catactgacg
      841 aacacgaaca acctcaccgc cagcgttgtg cgcacggctg ttccgttccc atcaaatatc
      901 cgatccgctg atgaaggtgg ctccacctgc tggctgtgtc aagggtttga tggccggcag
      961 tccccattcc tctgggactc agagtccgag cccacccttg aggatcacac cctcgatccg
     1021 gagccactcg atacatgcga ggctctcgat tcgggcgagc cctgtgctgc ggcggttcaa
     1081 caacatctgc gcaccacttg cacaaagttc tcgcccgtcg acgaacgaac cgagcgaacg
     1141 ggaggcgtcc gccagcacta agctccgcga aacgcggatt ctcacgacag ccattcggag
     1201 atcttcctcg gcctcggcat tgtcagacgc cggcccgtga tgagagcacc actagaggta
     1261 ccggacggac cggcggatcg tcgcggcgta cccgcaccgg aggctccggg agtcgtacta
     1321 cccggcggcg gacctccggg cgctcactca gcacctcgac cggcacttcg agtggaaggt
     1381 ggccgcggga ggcctcggct cgtccgggag gcggagtggc ccgagtaccg aggcctctac
     1441 gcgtcgaccg tccgcgacct cccgaacacg cccgagtacg gaaggctgaa ggcgtggtgg
     1501 aagcggctca cctccgcgac gctcgcggag gacgtcctcc agatcctccg cgacatcgag
     1561 ggcctcgccg ccccgaacga gttcgagccc ggcttcttct accggatgca gtggctcccg
     1621 tacgtcgggc tggccgccga ggccgagaca cgcgtcaacg cggccctcga ggccgagctg
     1681 ggcgggctag ccttcgcgaa ggcgcgggag tggaccgacc ggcagggcga cgaggcgcgc
     1741 gtggagctgg ctctggaagt ggcgtggctg agagatggag ctgtgactgg cccctgggga
     1801 ggccggcgcc gagttcatga ggagcgccga cgcctgggcc tacgacaagg tgcgaggccg
     1861 ttgaggcgcg ggcgatgccc ccgcgtcgtc gccgacacga agtggaagcg cctcgacccg
     1921 cggaaggccg acctcggcgt ctccggcgag gacgtctacc aggtcctcgc ctacgcccac
     1981 cgctacgcca ccgacactgc cgtcctcatc tacccccacc acgccgccgt cgggcgcgcg
     2041 ggatttcagc gggacttcgt gattcagggg gccggggccg cgcccgtcgt ggtccgggtc
     2101 attacggtcg atctagcgag gctggagagc gtggcggggc agatggggga ggcggccggc
     2161 gaggcgcccg caccctacgg ctcccgtggc gaagcccggc cgcgttccct tcggggaatc
     2221 gtttcgacgc ctaccgaatc tccgcccctc cgacgacgcc gaaacggcga acgtcccagc
     2281 gcttgagcat ctctttcgcc cggttgtagg ccggcgtctt catctgctcc gtccgaaccg
     2341 ccgcccgaag ccaggcgctc gcgtcctcga gtttcttctc cccggccgcc ttcaatccga
     2401 ggagataggc gacctcgcag cgacgagaag cgtcggtcgc cgttgcgagg agatcggcgt
     2461 ccgtggcctc accgaggagg aagcggccgt cgaccgcgtg gagacgtggc gcggccggat
     2521 cgctgaagtg ggcgcgaagt cgctcgaggc gagacccctc cggccttccc tcgaacgcgg
     2581 cggcctgggc ccgtagaagc cagctctttt cgtcgtccgg cagcagccac agaaggtcga
     2641 actcccgttc gtcgaaggcg gtcgtgccga cccaggaggc gctcctgctc gtgacgacgt
     2701 tcttgcggaa ccacgtgtcg gcgctctctg cgccgtgggc ccgccgccga aagcggtagg
     2761 cccggaggta gccgtcgatc ccctcgttct tcgcccgcga gatcgcgtcg aggagcacga
     2821 tggaggtctc ggggacgccg gcgcgctcga agcgggaagc gacctcgaag aggaaccagg
     2881 ggctggctcc cgcggcgagg agcgcctcga gggccggttt cgcttcctcc gtcggcctgg
     2941 cccggaagac ctccaggaac tctcgtggca cgaggacccg ccagtcctcc gcggagagcg
     3001 ggtagcgggg gtccaggagc ggcttcgcgg ccgcgtcgtg ccggccgagg cgccagagga
     3061 gcttcgcgag accggtccgt gccatcgagt cgtccggata cctctcgatg caggcttctc
     3121 ccatcttcag ggcctcgtcg gtcctcccga ggctcaacaa ggcctccgca cccgctccga
     3181 gagcgtcgcc cttccatgtc gggatgtgcg gctcgatcgt ggaccaggcc tcgtcgaatc
     3241 gtccctggag gaagagaacg tgctcgagac gcgaggcgta gacggccttc gagagggccg
     3301 gcgcgtccgg gtgcgagccg agccagagcc ggaggaacct ctcggcgtcc tcgaggaagc
     3361 cgtgctcgga gagcagccga acggacctca gcgccgacca ggtgtcgggc ctgctggcca
     3421 ggatgtccag gaggacacgc tggagctcgg tgtcgggaat cgcggaacgg tcctcgaggt
     3481 actcgacggc gatgagcctc ggccacgcgg cgaccgccgg atcggatgcg atggccgtga
     3541 gccgcgggat atcgcccagc gtgtcggcga gccagacgtc cgtggcgctg gccgtgctac
     3601 cgggtgtgga ggccgagagg tagtaggcgc gcatggcgac cgggtcgaag agcgcggagt
     3661 ggcagatccg cgagaacagg aggcggttcg cgggccggct gtcgaggagg tgcgcgagct
     3721 ttgccccgac cctcgggagc tccggagccg tggcccagag ggaggactgc agcgcgtcgc
     3781 cgagtcggcg cagggccgcc tgcccgagac cggggagggc gtcgaggtcg ttgacgagct
     3841 tcgcgacggg catccggccg ctcttcaccg cggccaggtg gtcgtagagc tgccggaatt
     3901 gggcgccgag tccggattcc gcgcccgcga ggagctgcgc gaactccgtc gccccgggac
     3961 ccgacgagag ctcgtcgagg tagaagtcgc cgatgtcggc gaggcccgag tagaagaggg
     4021 cgcgcaaccg ggcgttccgg gcgcctgagt cccagaacgg accgtccgcg ggcggaaggg
     4081 aagcgaggga ccgctcgaac gcgggaacga cgggggaatc ccgcggcacg ttcgcgatgc
     4141 cggcctcggt gaacgcgact tcgatgaggg cgaagcccag cgcggacttc tcgccgaggt
     4201 cgcccccgcg gcaccgacga aggaggtgga cgccgggcgc gccgttcgag gatccgagcg
     4261 ctccgagccg gtcctgcatc tgcgcggcgc tcagggagcg cgcttcctcc aggaggtcca
     4321 ggtaccgcgc gtccggggag gtgtcggctg cggaaccggt tgggccgtcg tgccgctcgt
     4381 taccggggat gtggggccgc gggaaggtcc gcttccagga cgcgtcgcgt cggaccatcc
     4441 ctggagacga gtaccccatc gcgtcggaaa ggaccccggc ccgctcgtcg cggtggacgc
     4501 ccgacgcgac ttccgagagg gcaaggagac cgagggcgcg ccccgcaacg aggtccgcca
     4561 tctcgatcga gtcggagagc tgcacgagga gggtcacgta cgagagagag gcgaggtcga
     4621 ggagaaggcg ccggtcggcg ccggaggtcc agagccggtc gacctcctcg agcgcgcgga
     4681 tcggaccatc agtgaaggcg tcggcggcca acgcgcggag gcgcgacgcg tccgcctcgg
     4741 agaggggcgt cccccccttc gggacatggc gattcacggc cgcggcacgc agcgcgtcga
     4801 gggcatcctg gaaagacgga atgccgggca gctcggccgg ccgcgagaac ccgacgtcca
     4861 cggaccatcg gcgcttcgac acgctcaagg tggcctgtcc ggcgccgccg caaaggcggc
     4921 tcagctcctc gaaggcgagg atgaaccgac gatcgggctg aaggagggag tcgttctcga
     4981 cgacatcccg catccgctcc ggcggtacgg tgagaaccgc ggacggcgcg cgggtcgggc
     5041 ggggccgagc gccttcggac gcgagatccg gggcatcgga cgagcgctcc cggacgacgg
     5101 cgagtccgat accggctgcc gcgacgaggc cgaccagcag gctcaggagc agggtccggc
     5161 gggaattgct cgaggcgttc atcatgagga gactaccgcg ggcctcgcgg gagatccggc
     5221 acggacgagc gcccgtcggt cgggaagtcg tctcgaggcc tgccggatct ccgcccctcc
     5281 gacgacgccg agacggcgag cctccctgcg cgcgacgcgc gaggctcttc ggcgactcgt
     5341 acgacctggt ctctgggttg aactgtgcac ggtctggcca gtcgggtgtc cgaaagcgat
     5401 gcgatcaagt catttccgat gccgatggag cggcctgttg gagttttcgc ggcaggagca
     5461 gcttaggtgg agaggtagtt gatggcgcga ggagaccacc agaaggcccg tctctcgatt
     5521 tgtcggcgaa gccgccccct acagccgttt ctcgtcgttc ctcgaaatgg gttcctctct
     5581 ggcaccaaca acaggaaagg agccgtcgca tgcccagtcc gaaagcgcag ctgaagcccg
     5641 ccgtcggcct cgcgagcctt cccaatatcg aactcgagaa gacggatgtc gacgggatcg
     5701 tcgcctcggc gacgaagtcc gtcgcctcga gcatggggat ccggccggag cagcttcctt
     5761 cgaccgtcgt ggacgcgatc aaggtctcgg cggcgaccgg gatcagtact cggaccaagg
     5821 ccctcatcca gcgcaacgtg gactccagcg tgaaggacgc cgtcctcaag ggggcgaaac
     5881 cgctcgaaat gctcgatgcc cgcgttcagg cggcgagtga cggcatcgct cacatcgtgc
     5941 aggacgggaa ggtcgacgcc gtcctcgcgg acacggcgaa gctcctctgg aagaagtacg
     6001 cggccctcaa gacggcggga ttcaccgata cgcaggcgtt cgacctggtt cttgccgagg
     6061 tccagggccg cgccgggcga aacagatagg tgagcccgtg agttggtgcg ccttcgtctg
     6121 cgacgcggta gacggcgcgg ccgggtgggt cgaggaccag gcagaggacg ccgtcgggag
     6181 cgtggtcgac tcggtcgagg acgggctttc ggccgtcgtc gactacttcg aaggtgtcgg
     6241 tgaatcggtc gaagaggccg tgtcggatgt cgcgcagctc gctgccgacg tcgccgaggc
     6301 cgcgtggaac gggatcaccg aggccgccag ctccgtctgg gactggataa agagggtcgc
     6361 cgggaaggtg tgggagtgga cgagcggcgc gctcgaagac gcctggaact gggcccgaga
     6421 agccgcctcg gacgcgtggg actggatttc gggtgcggcc gagtcggtct ggggcgccct
     6481 ctgctccgcc tgggacgcgg tctccgggtt cgtcgaagag aaggtgattc ccttcctcgt
     6541 cgacgcgctg tgggtcctga cgcacgtcga cgacgtcctc atcgccgtta gcctggggct
     6601 ccagtgcctc ctgacaggcc aggacgagag ggagtacgac ttcatcgagg ggctctatct
     6661 cctcgacgaa gccgcgctcg cccaaaggaa ggtcacgttt ctccccgttc aggacgacta
     6721 cgtggtgttc agcgacatcc acatgttcgt ggcgggggat ccgctcgacg agtttcggca
     6781 gctcgggaac agcgagctct accaggccgt gctcctcttc tactgtaagg gcggctacac
     6841 gctcgtggag aacggggaca tcgaagacct ctggatgcgc gagacgacgg tcggcgaggc
     6901 cctgctcgac caggcgacgg acatcctggg gtggccgttc ggggagatga tcgaggacga
     6961 gcacgagaag taccggatcc gatcgcaggc ggtgaagatc ttcgacaaca actccgacgt
     7021 ctatcagacg attcgcgcgc tgttccacga ccagcggaag ctcgtgcgcc tgctcggaaa
     7081 ccacgacgac gcctggcgca gcgacgacta cctgccgggt ctgcaggaga tctacccgga
     7141 gttggaggtc tttgactacg cgctgctcgg agactacggc ggcaccccgc cggcctacgg
     7201 gaaccatccc cgggtcatca tcgcccacgg ccaccagctc gacgcctgga acaactccat
     7261 ctgccgggcg gccggcgcgg cgatgacgga gtgcgcttcg gggattcctg ccctggcaac
     7321 gaaagtgacg gagcgctcgg aatgggagcc gacgctgctg tggaaggagg gctttccgaa
     7381 cgagctcaaa caggacattg ccggagtgga cgaggtcgag ttctacgaga ccgtcgagga
     7441 cgactttcga agctactcgt acgtgcccca gttcatcctg ggccacaccc acaggccctt
     7501 ggaggatccg cagatcccgg agtggaagga ctgcgacgaa cggaagttca ccgagtacac
     7561 gaacgacggc accgccgggc gttgggaaca gttcatctgg tgcgcgacgg tcgagaacgg
     7621 ctcggtatcg ctctacgggt ggacgtggga tgccgccggc aagcccttcg cccgtcgatt
     7681 cgtcggcgca ccggccgact gcctgcggcc ggtctaggcg ggagcggcga ggaggatggg
     7741 aatgccacgc gacgagaagg gcgagaaggc tggtgatccc gccgacacag acgggctgtc
     7801 cgctgcggca ggtgctctcg actccacggg cgtgttgacg cttccgccct cggaccgggt
     7861 gtcgttggct gcccgagcct gctgtctcaa gaaggtgctg gagaggtgcg ttggccgctc
     7921 tatgcccgac ccattgggcg ggccgctagc cctgctgtag ccatcacgac gaggtggaga
     7981 tgaatgtgcc cgccgttgac agtgagagaa gccgcgttct catcctgagt ttccatgcgg
     8041 cgcgagcgat acctggaaag actgtcgccg ggctgttcga cgaggatgtc cttttcaaga
     8101 agcacgccag cctacgagga cttctcgagc aggatgccat ggatgagcac atcgcacccg
     8161 agtccatcgt caacgacgat gagcacgggg gcgtcgagaa ggtggagcat gacggagcgt
     8221 cggcttggac cctgagcgca gtcacgcggc gggtgtggga cagggtcggc tactccaatc
     8281 cgagcacaca cgagtccgac ttttccatgt ggtttcaatc gttatggacc caaccaatcg
     8341 actgcctcgt gatcgccggg caccacggtt gggccaaaga ggtggggccc ttcttctggg
     8401 ggagtgaaca gccgaagcgg ggaggcgacc atcggccgta cacgtcgatc gacgtccatc
     8461 ccgaggatga cgctccggca atcggcgtca gcggctactc gtactcgagt tcccggctcc
     8521 gcgagcgcat acggcccttc ctcgtgatgc acgcgctctc ccgctgctcg ctgctcgtga
     8581 tcatgggctg caacggcgtc ccggctcccg cggacactct gcggatgggc acggcctggc
     8641 aggactgggt ccggtccgcg aacccgcaga acgagagacc catcgtcctt ggctggtggg
     8701 ccaagcacca gatgccgcgc aacacccgga agggcgagaa tcccgagcac ttcagcgagg
     8761 acttctggac gcggatgaaa gggctcgctg gaggtcttca gggtcctcgg cccctccaaa
     8821 cactctgccg cacgaaacct aaggccgtca tcgaagcctg ggccgcctcg atgaagggcg
     8881 cgttcggtgc gagcaagacg ggccaacgcc atctctggtt cgagacgggt aagggtgtcg
     8941 gggcgatcga cccggacggc gctgtgtggc gcgtcacgcg acctgaagga ccgatcgaga
     9001 aggacaagag ctcatgaaga aggtcaacgt tctcaccctc gacgacgtcc tcgagctgtc
     9061 aggcccatcg ctgaacctcg gggcgacgga ggccaccggg gccacccgtg tcgtgcttcg
     9121 cgagggggga gaggagatcg agttcttcac cgacgcctcg tgcgacggcg ggccggcgga
     9181 actcgaccgg cggacgtcgc ttacgtggga ccgaggagac gtggcggtct cgaagggccg
     9241 gctgactcta aaggacaagg aggaagtcga gctcacgttc cactggccga accccctcac
     9301 gccggcgggg agcgggctct ggctgcgtct cgcgatcgtc cgcgacggcg caggccactg
     9361 ggtgcgaatg gcccagcagt atccagcgac aggagcgacc gtctggtggc gtcgcctcac
     9421 gtcgaggccc gcgtgggagg agtaccgcct ccccttgcgg gtgccaaggg tgagtgtcgc
     9481 gcagctcctc tccgtctgga agaaccaggg gttcgggtac gccagcggcg acccggccgc
     9541 cggttcgtgt ccgctggtgg ccggtgccga cggtccagcc tgcctcgtcg agttggacgc
     9601 ccgggaacgg gggcgagggg tcttcctcct ggcggcagag aagcgctggg cgtcggcccg
     9661 ctttagggcg gaaggggttc gggcgcggac gggccagcgg gcgctgggtc tttcagagct
     9721 cggtttcgcc ccgcgcccct acgtcttcca gggagatctc gatctccgct ccgcaggtgg
     9781 gccgcgcgag ttcttcctgg aatggccggg cataccggcg ggcgggcccc tcccatccga
     9841 ggtgctgagc cttcaggccc tttggcagga ggtcgcgcag cactacgcgc tgggcctcgc
     9901 cgcaacaagg gcccgccggc acctcacgtt cgtgccgacg gaactccaag ggcggagcga
     9961 cccggtgaac ctacacattc gggttgcggc tgatccgcgg agtgcaggcg ggccgcccct
    10021 atggaaagca aacctctttc agatcggccc cgtggactgc cggctcgacc tggttctggg
    10081 cggggcgcgg acggaggaag gctgcgcgct cagggtcccg gtgtgggctc gaagctcgga
    10141 gtggaaaccg atcgtagatc acctccacga ccggtttgtg aaagaacgac cgaccatccc
    10201 gccgctcctg ggcctgggcc tggacctcga ctccgcgtcg gacaggccgg tgtcgagtgc
    10261 gctcgtcgtc agcggtgtgc gattcgacgt ccagggcttc gagcgcggcc gactccggtg
    10321 cacggcggag cccggagctg acgtacacgg acgccccccc ctcggggtcg atcttcgcct
    10381 cgacttcacg gtatccaccc cacgtgccgc gactcaggat cccgagatcg gcttcgagac
    10441 gcaatccagc tggctgggtc gggagcggcc gatctgcgtc gacttgagtg aggagacacg
    10501 gacgacgttc gacctctgtg tggtggagcg ggcgaccggg gggctgtcgc gcctcctccg
    10561 cctgacggtt cgcgcgccgg aggggcttgg atcgctgacc acagacacct tcgtcctcga
    10621 cccgagcccg ctccaggtcg cacgggtcct gaccgcgaac aagctcgagg agggcaccgt
    10681 cctcgcggag tacaccgatg actcggacct tcctcccgag tgggccttcc gcacggagac
    10741 gggccagatg gacatcgtcc tgcccccgca ggtcatcggc gaggaaatga tcaaggggaa
    10801 gctcgccgtc ggcgggaagc cggtaccgga tccgaacgcc ctcttcgact tccgtctgag
    10861 tgcgccagcg tacctgcggc tggaccgcac cgacgtcgac gccgccagag ccagcgcgcc
    10921 gtgggcgctc cggcgcctcc tctcgcagcg gctcggtgtc accggcgtgc gcctgcagga
    10981 ggcgatcttc gagctcctct acggcctcac gacgacgctg cgggcgcccg gcttgcggat
    11041 cgcggaactg gacgccttcg tcgggcgtgt tcccttcgcc gacgagctct gcgcgctcct
    11101 cggcgtcgag atcgaagcgc cggacgccac gggcgagctc accgtcgcgg agctgcgaag
    11161 gaggcacgcc acggacctgg cgcggtggat ccgggccctc ctccaccggc cggcccagat
    11221 gcccgtcttc cgcgagtgga cggatcggcg gaagctcacc cttttcgagg gagtcggctt
    11281 cgccctgcgg ccaagtcggc agacggcgca ccccttcgac ctgacgtctt tcgcggatcg
    11341 gacccccccg gaccagcacg ataaggcgcg tagacccctc cgcggcggcg tcgacttccc
    11401 gttcgagacg cgctccatct acgaggcctt ccgccggagc acgacgccga acgacggaac
    11461 ggtctcaggc ctcacttttg gaagcctcgg tgggaccggc gggcagcagg ccactcctgt
    11521 ccaagataga tccgtgatcg cgtgagaaag gccctccgga cggcc
//
LOCUS       DAOONL010000102        11514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6281_length_11514_cov_15.161532, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000102 DAOONL010000000
VERSION     DAOONL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_11514_cov_15.161532"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..4209)
                     /locus_tag="PLP50_13055"
     CDS             complement(127..4209)
                     /locus_tag="PLP50_13055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52519.1"
                     /translation="MAPPSPDPFPERRLLPYETTRLEARRVLVLAPHADDETFGCGGA
                     LAALLDGGARLDVLVVTDGAGDEADTAARRLVGERRLEETREALRRLGGGAARCAFLP
                     DRGLGLAGESLAAALEGEFLATRPDLVFVPSPVEIHPDHRAVAEAFLSLFAGEAGRRF
                     AAALPDGARAAFYEVSQPFRPNVLVDVSRTVARKEAAVAAFVSQLGGHDYAAFARGLG
                     QYRRMSLPRSVASAEGFFLVALAQLPSLDPLRLARAIGPTLSVEELLPPQARSVEEET
                     PSGAAVVAPVAEAQARPLVSVVVRTKDRPELLAEALESVASESGVPLETVVVNDGGAD
                     VADVLARFPQLRLRLVDLRPGRGRAAAANAGVEAATGRFVHFLDDDDLLLPGGLPALA
                     AEAREGEVVYGRVEAFRWSGTGEGRLRTPFRSFAEPFDEAALLLENFIPMSAPLLPRE
                     ALLGAGGLDAALQRFEDWDLFLRLSQTHRFRLVDVPVSEYRVFPGHFVDDPDAATTQE
                     RHRVDVLRKHAGRYGPETLSAILLHVKKDLLPREVRREVAAVERLLSHATEESARERD
                     RLEAERTDAERRAGRTLATRASVVVVNFDGRHHLEKCLPALEASDPKPGEVILVDNGS
                     SDDSVAWVREHHPAVKVLPMGANLGFGEANRRGALEARGDFLVLLNSDTVVEEGWLAG
                     LLAPLEAEPEIAATCSTLRLLATPELLNGLGGGMTRLAYAFDHGYRFPYAPWPPSGEE
                     PRWHDVLFPTAAAMAMRRHEFFGLGGFDPAFFMYHEDVDLGWRLWILGRRVVVCRDSV
                     VRHAFLGTSKAVRGLDWRLKLGVRHAVRSILKYVEPLEAAKVLRWHAVVLARAGAWRL
                     LGHAVLWNLRHLPGTLRLRAAMSAGRKRSSEDLRRAGLVLRALQPPPPPEAPRFREEG
                     PDAWIVSNVLLPGEPSGESRLGWGWYGPEHDGTASFRWTVGDARLGMRVAAGAEGRLR
                     IAAIGSPAAPAGPLRVTCNGREAEAALEPGAWREVEVQVSAGADGLLDVVLRSPSGVP
                     DEVHGSWDFRRLGAGVRSVAFVPASAEERHPPARLSVVIPTYERKAVLEETVRALAAQ
                     SARPFEVIVVDDGSNDGTTDLLHRLREELSDRLDLVPLRQPNLKQGRARNLGVARANG
                     ELVLFLGDDTIPEPACVAEHLAAHASRPGPCAVIGFTGWHREKMRVTPFLDFVNGHGP
                     QFSFDLLRDGQEVPFTTLYTSNVSIPREHLGAEPFDHRFTSYGWEDCELGWRLSSAGL
                     PIVYRKAAATRHVHPQTMAQFLARQAHVGRAIDVLYEIHPELEGNRWLPPPRPRFRHR
                     AFSFAYRALGRVGALLDSAGVPLPLRAYHALVGWAFHSGR"
     gene            complement(4253..5314)
                     /locus_tag="PLP50_13060"
     CDS             complement(4253..5314)
                     /locus_tag="PLP50_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52520.1"
                     /translation="MISAVFVSYRSATLTARAIATFREEARRAGREAEAVVVVNSGDA
                     AERDALVPHADRVLFPPRNLGFAGGLNAGIAAARGGTLLLANPDLLFCEGSVAALAAA
                     VEAGGPVAAGPALWADAARSVLLPPAEEGRPEELARRALAADPARADRVFRRDARRAA
                     AQAERAAAGASAVVRGLSGAAVAVTRATLGAVGPFDEGYPLYYEENDWQRRLLLRGGR
                     LVFVGGAHVVHLFAQSTKTEPRAGSWFAESEARYYATHFGEAGKRGLARLAACAPFEA
                     PPLPAAEGLAWSDPAPAAVAISPFRHFRPFALALVPTDERRWTPPADLAEAHSGEALF
                     LRAFARATGATLAEVRFAG"
     gene            5338..6303
                     /locus_tag="PLP50_13065"
     CDS             5338..6303
                     /locus_tag="PLP50_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52521.1"
                     /translation="MAIEFRPAGKSDRAAILELFEVAFKAPANAADLAWKYDVNPHPA
                     LSALAIEDGRALGFIGALGTRYRGEAMDQRGNTVVDVMTRPDSRALGRAGMFKRLGEL
                     FRRINAEAGTLFDFGFPNERARKIEERLFGCVLAEPCAERGRRLDAPPLLGRLRRRLL
                     RVSEGEPFGRAHEPLAELLHARPGWRTDRSVRTLAWRFSRPGYAYRTFQLLDWRGTSR
                     GYAVVAIREGRAALVDLQVASESDGTLVDLLAAVQERLGAIAADRMVLRSPQRGLLAS
                     RLEGELAFVPEPSDTVLLMHPITDGYDFLSPGATLDYRFADHDVF"
     gene            complement(6323..7309)
                     /locus_tag="PLP50_13070"
     CDS             complement(6323..7309)
                     /locus_tag="PLP50_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52522.1"
                     /translation="MSDAVPQGAELEVRALFRDEAAEAHAERRARLASDDPDRWEGIA
                     FPVEFALELAAVCNLACVMCPVPTTSRKRRLMDEALFRKSVDEIASETGFVLFPQGFG
                     EPMLHPKWAELLAHAKAKGIGPIVVLTNGTLLDERNVARLLDLDLDAVVVSIDGVEPE
                     TYAAVRVGGDLGVVEANVRRLLTARGPAPRPRLVLRIIRMKETAAQIAAFFERWRPLL
                     GPSDEIAINEFNDWAGKVEDRSDASRPQPAAATMRRPPCRMLWMNLSVHADGKVSACC
                     HDSEDELLVGDLAKGDTVRGIWEGPALARLRRIHREGRFEELPICHACRNQA"
     gene            complement(7306..>8178)
                     /locus_tag="PLP50_13075"
     CDS             complement(7306..>8178)
                     /locus_tag="PLP50_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52523.1"
                     /translation="AGHCQLDCDYCIIHPVVKREPSLVYEDLRFFLDEVGTRTFFIFS
                     GKGDFFAGYPKRDRFLDRLLDHDVEVALDVNGVLLNEYPELSEEKLAKVRAVNLTLHY
                     KQVRDKRVEKAWEANARTILEKHRGELILGTIFSPLLSDLWEEGLAYYRDTVFAATGR
                     KVWAIRDCDRPFDAAQEELFSSLLERYAAVVEKEHKDDFSAPFAGKEAVLCPAGRDYF
                     RIWNDGRVQGCPWIPGLADLGNVKERRLARRAAPFRCTTPSFCDCYDIHQLGKMGYED
                     ATPALPVLSSGTAR"
     assembly_gap    8179..8278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8279..8769)
                     /locus_tag="PLP50_13080"
     CDS             complement(<8279..8769)
                     /locus_tag="PLP50_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52524.1"
                     /translation="MKVVYLLNGTALCGGVKVVYQHVAILRRLGVDAEVVSTTPPSDW
                     APESWSFYRQVPDLTPEAIGPADVAVGTMYHTVPQAMAVPGARAFHLCQAYEAIYEPA
                     RPLWPEIEEVYRLPATKLVVSPHLADLVRELYGQEAIHIPQPFDADAFSPRSGERPQD
                     GTFR"
     gene            complement(8766..9947)
                     /locus_tag="PLP50_13085"
     CDS             complement(8766..9947)
                     /locus_tag="PLP50_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52525.1"
                     /translation="MSDAAFAFEGLSKVYRLYASPRDRLLEAITGRPRHTPVHALRGV
                     SATVLRGSVLGVIGENGSGKSTLLKILAGTTEPTSGTVHLPGRVAAILELGSSFHPEV
                     TGRRNAVLQAALAGLTKAEIAAALPEIEAFAELGDFFDRPVRTYSSGMTMRLAFAVAT
                     AVLPDVVILDEALAVGDGRFQKKCVDRLHDLRSRGRTIFLCSHALYYVSTLCETAIWL
                     RDGEVAALGEAQKVVLQYEEYLARKEQERTVAAPAALQDSLGRFTSIRLLDGAGKETA
                     AFAPGDPWVLELAFEGDTPDRPLHVHVAVVTPDQVTCFVADSKVSGTGPFVGKARHEI
                     RLSVESLPLAKGEFILVIFLGDEKGLALYDARSDVRFRVESDRWTSGFMTVPVGWETL
                     G"
     gene            complement(9940..11061)
                     /locus_tag="PLP50_13090"
     CDS             complement(9940..11061)
                     /locus_tag="PLP50_13090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52526.1"
                     /translation="MNASSPDDVSRSSAPLSGAPRLVASPYLHFDGWQVVNPLTNVTL
                     TPTDRLFAPLHELRGGRPVDGAIERELRAGLWAIPDGEDLSHRFRLKVVSLEAHSVCN
                     QACYFCPVSVAPREKSFMPTELYERIVAELSAFRDSIEGVCMNNYNEPTVDRRFVQQV
                     ATIKAAGLPPAVLSNGSGLTPDRVDALLGLGGLRYLSVNISTLEKDRYARDRGVDQLE
                     TVVRNIDYMCDKPVAEEMDLVVLGGGDEPHRKAHREITERYGGSRFHVRPFEARDRAG
                     WLPNGVRPAEKHRKLRGCDNVGSRPLEHLHVTAEGKCIFCCEDYDEKLVVGDLKTQSV
                     REVLEGPALALLRRWSYGVEEAPDDFICRSCIAARTEGE"
     gene            11147..>11514
                     /locus_tag="PLP50_13095"
     CDS             11147..>11514
                     /locus_tag="PLP50_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52527.1"
                     /translation="MPPRTVPARPRPARGALLAVAAALLLSADDRHAGKVSDGRQTIG
                     TAVAIVETGELGIGKGAPRAFGREADAVSRYGLGASLAQLPAAAFAPAVESAFGPGTS
                     QPLFLLAPLAGVLLAAAAAGG"
BASE COUNT         1586 a         4109 c         4256 g         1463 t
ORIGIN      
        1 tgggtcatgg cggcctccgc tgcggcccgt cctccccgag ggaccggtgg gccgctcgac
       61 gagtcagaag caatctccct gccggctcgg cccgtccagg cggcgggacg ggcgccgacc
      121 ggacggtcag cggccggagt ggaaagccca cccaacgagc gcgtggtagg cgcgcagcgg
      181 gagcggcacc ccggccgagt cgaggagcgc gccgacccgc cccagggcgc gatacgcgaa
      241 cgagaaggcc ctgtggcgaa agcgcggacg cggcgggggg agccagcggt tcccttcgag
      301 ctccgggtgg atctcgtaga ggacgtcgat cgcccggccg acgtgcgcct gccgcgccag
      361 gaactgcgcc atcgtctgcg ggtggacgtg ccgggtggcg gcggccttcc ggtagacgat
      421 cggcaggccc gcggaggaga ggcgccagcc gagctcgcag tcctcccagc cgtacgacgt
      481 gaagcggtgg tcgaacggct cggcgccgag gtgctcgcgc gggatcgaga cgttcgaggt
      541 gtagagcgtc gtgaacggga cctcctggcc gtcgcggagg aggtcgaacg agaactgcgg
      601 accgtggccg ttgacgaagt cgaggaacgg cgtgacccgc atcttctccc ggtgccagcc
      661 ggtgaagccg atcacggcac aggggccggg acgggacgcg tgcgcggcga ggtgctcggc
      721 gacgcaggcg ggctccggga tcgtgtcgtc cccgaggaag aggacgagct ccccgttcgc
      781 ccgcgccacg ccgaggttcc gcgcccgccc ctgcttcagg ttcggctgtc ggagcgggac
      841 gaggtcgaga cggtcggaga gctcctcccg gaggcgatgg agaaggtccg tcgtcccgtc
      901 gttcgagccg tcgtcgacga cgatcacctc gaacggccgg gcgctctgcg ccgcgagggc
      961 gcgaaccgtc tcctcgagga ccgccttccg ctcgtacgtc gggatgacga cggagagcct
     1021 cgcgggaggg tgacgctcct cggccgaggc cgggacgaat gcgacgctcc gaacgccggc
     1081 cccgagcctg cggaagtccc agcttccgtg gacctcgtcc ggcaccccgg acggggagcg
     1141 gaggacgacg tcgaggaggc cgtccgcgcc cgcggaaact tggacttcga cctcacgcca
     1201 ggcgcccggc tcgagcgccg cctccgcctc gcgcccgttg cacgtcaccc ggagcggacc
     1261 ggcgggcgcg gccgggctgc cgatcgcggc gatgcgaagg cgcccctcgg ctcccgccgc
     1321 cacacgcatt ccgagccggg cgtcgccgac ggtccaccgg aacgaggccg tcccgtcgtg
     1381 ctccggcccg taccagcccc agccgagacg gctctcgccc gagggctcgc ccgggaggag
     1441 gacgttcgag acgatccagg cgtcgggccc ctcctcgcgg aatcgcggcg cctcgggcgg
     1501 tggaggcggc tgaagcgccc gaaggacgag gcccgcgcga cggaggtctt ccgaggaccg
     1561 cttccgcccc gcgctcatgg cggcgcggag gcggagcgtc cccggcaggt gccggaggtt
     1621 ccagaggacg gcgtgaccga ggagtcgcca cgcgccggcc cgggccagga ccaccgcgtg
     1681 ccaccggagg accttcgccg cctcgagcgg ctcgacgtac ttcaggatcg agcggacggc
     1741 gtgacggacg ccgagcttca gcctccagtc gagcccccgg acggccttcg aggtcccgag
     1801 gaacgcgtgc cgcacgaccg agtcccggca gacgacgacc cgccgtccga ggatccagag
     1861 cctccagccg aggtcgacgt cctcgtggta catgaagaac gccgggtcga agccgccgag
     1921 gccgaagaac tcgtggcggc gcatcgccat cgccgcggcc gtcgggaaga ggacgtcgtg
     1981 ccagcgcggc tcctcgcccg acggcggcca cggcgcgtac gggaagcggt agccgtggtc
     2041 gaacgcgtag gcgaggcgcg tcatcccgcc cccgaggccg ttcaggagct ccggtgtcgc
     2101 gaggagccgg agcgtcgagc aggtcgccgc gatctccggc tccgcctcga gaggcgcgag
     2161 gagccccgcg agccagccct cctcgacgac cgtgtcggag ttcaggagga cgaggaagtc
     2221 gccgcgcgcc tccagcgcgc cgcgccggtt cgcctcgccg aagccgaggt tcgcccccat
     2281 cggcaggacc ttcacggccg ggtggtgctc gcggacccac gcgacgctgt cgtccgacga
     2341 gccgttgtcg acgaggatca cctctcccgg cttcgggtcc gacgcctcga gggcggggag
     2401 acacttctcg aggtggtgcc ggccgtcgaa gttgacgacg acgaccgagg cccgcgtcgc
     2461 gagcgtccgc cccgcgcggc gctccgcgtc ggtccgctcc gcctcgagtc ggtccctctc
     2521 gcgggccgac tcctccgtcg cgtgcgacag gagccgctcg accgcggcga cctcccggcg
     2581 gacctccctc ggcaggaggt ccttcttgac gtggaggagg atcgccgaga gcgtctccgg
     2641 cccgtagcgg ccggcgtgct tgcggaggac gtcgacgcgg tgccgctcct gcgtcgtcgc
     2701 ggcgtcgggg tcgtcgacga agtgtcccgg gaagacgcgg tactccgaga cggggacgtc
     2761 gacgaggcgg aagcggtgcg tctgcgagag gcggaggaag aggtcccagt cctcgaagcg
     2821 ctgaagcgcc gcgtcgagac cgccggcgcc gagcagcgcc tcccgcggga gtagaggcgc
     2881 gctcatcgga atgaagttct cgaggaggag cgccgcctcg tcgaacggct cggcgaagga
     2941 acggaacggc gtccggagcc gtccctcccc ggtcccgctc cagcggaacg cctcgacgcg
     3001 cccgtagacg acctcgccct ctcgcgcctc cgcggcgagc gccgggagcc cgcccgggag
     3061 gagcaggtcg tcgtcgtcga ggaagtggac gaagcgcccg gtcgcggcct cgacccccgc
     3121 gttcgccgcc gccgctcggc cacgccccgg ccggagatcg acgagacgga ggcggagctg
     3181 cgggaaccgc gcgaggacgt cggcgacgtc ggcgccgccg tcgttcacga cgaccgtctc
     3241 gagcggtacg cccgattccg acgcgacgct ctcgagcgcc tcggcgagga gctcgggacg
     3301 gtccttcgtc cggacgacga cgctgacgag aggccgggcc tgcgcctccg cgacgggagc
     3361 gacgacggcc gcgccggacg gcgtctcctc ctcgaccgat cgggcctgcg gcgggaggag
     3421 ctcctcgacg gagagcgtcg gaccgatcgc ccgcgcaagg cggaggggat cgagggaggg
     3481 cagctgggcg agcgcgacga ggaagaaccc ttccgccgac gccacgctcc ggggaaggct
     3541 catccgccgg tactgcccca gcccccgcgc gaacgccgcg tagtcgtggc cgccgagctg
     3601 cgagacgaac gccgcgacgg ccgcctcctt gcgcgcgacc gtcctcgaga cgtcgacgag
     3661 gacgttcgga cggaacggct gggagacctc gtagaaagcg gcgcgcgcgc cgtccgggag
     3721 cgcggcggcg aagcgacggc cggcctcgcc cgcgaagagg gagaggaacg cctccgcgac
     3781 cgcgcggtga tcgggatgga tctcgaccgg cgaggggacg aagacgaggt cgggtctcgt
     3841 cgcgaggaac tcgccctcga gcgccgccgc gagcgactcc ccggcgagac cgagcccgcg
     3901 gtccgggagg aaggcgcagc gcgccgctcc tcccccgagc cggcggagcg cctcgcgcgt
     3961 ctcctcgagc cgccgctcgc cgacgagccg ccgggccgca gtatccgcct cgtcccccgc
     4021 gccgtcggtc acgacgagga cgtcgagccg cgcgccgccg tcgagaaggg cggcgagcgc
     4081 cccgccgcag ccgaacgtct cgtcgtcggc atgcggcgcc aggacgagga cccgccgcgc
     4141 ctcgaggcgg gtcgtctcgt aggggaggag tcgtcgctcg ggaaagggat ccggggaagg
     4201 gggcgccacg cggcgcgatt ctaccggccg ggggtcccgg cgagccgccg cttcatcccg
     4261 cgaagcggac ctcggcgaga gtggcgcccg tcgcccgcgc gaaggctcga aggaagaggg
     4321 cctctccgga gtgcgcctcg gcgaggtcgg ccgggggcgt ccagcgcctc tcgtcggtcg
     4381 gcacgagggc gagggcgaac ggccggaagt ggcggaacgg cgagatcgcg accgccgccg
     4441 gggccgggtc gctccacgcg aggccttcgg cggcggggag cggcggcgcc tcgaacggcg
     4501 cgcaggcggc gaggcgcgcg agacccctct tccccgcctc gccgaagtgg gtcgcgtagt
     4561 acctcgcctc cgactccgcg aaccaggagc cggccctcgg ctcggtcttc gtgctctgcg
     4621 cgaacaggtg gacgacgtgc gcgccgccga cgaagacgag ccggcccccg cgcaggagaa
     4681 gccgcctctg ccagtcgttc tcctcgtagt agagcgggta tccctcgtcg aacggcccga
     4741 ccgccccgag cgtcgcgcgg gtgacggcga cggcggcccc ggagagaccg cggacgacgg
     4801 cgctcgcgcc ggcagcggcc cgctccgcct gagccgccgc ccgccgggcg tcgcgccgga
     4861 agacgcggtc cgcgcgcgcc gggtcggccg cgagcgcccg gcgcgccagc tcctcggggc
     4921 gcccctcctc cgccggggga aggaggacgc tccgcgcggc gtcggcccag agcgccggcc
     4981 cggccgcgac gggtccgccg gcttcgaccg ccgcggcgag ggccgcgacg gagccctcgc
     5041 agaagaggag gtcggggttc gcgaggagga gcgtcccgcc gcgcgcggcc gcaatcccgg
     5101 cgttcagccc cccggcgaag ccgaggttcc ggggcgggaa gaggacccgg tcggcgtgag
     5161 ggacgagggc gtcgcgctcg gcggcgtcgc ccgagttcac gaccacgacc gcctcggcct
     5221 cccgtccggc gcggcgcgcc tcctcgcgga acgtcgcgat cgcgcgcgcg gtgagcgtgg
     5281 cgctgcgata ggagacgaag acggctgaga tcatcgggtg gtacccttcc gcgcgccgtg
     5341 gcgatcgaat tccgaccggc cgggaagagc gatcgggccg ccatcctgga gcttttcgag
     5401 gtggcgttca aggcgccggc gaatgccgcc gacctcgcct ggaagtacga cgtcaacccc
     5461 cacccggcgc tcagcgccct cgcgatcgag gacggccggg cgctcggctt catcggggcc
     5521 ctcgggacgc gctaccgggg cgaggcgatg gaccagcggg ggaacaccgt cgtcgacgtc
     5581 atgacgcgcc ccgactcgcg cgccctcggg cgggcgggga tgttcaagcg gctcggcgag
     5641 ctcttccggc ggatcaacgc ggaggccggg acgctcttcg acttcggctt cccgaacgag
     5701 cgcgcgagga agatcgagga acgcctcttc ggctgcgtcc tggccgagcc gtgcgcggag
     5761 cggggccgcc ggctcgacgc accgccgctc ctcggacggc tgcggcgccg gctcctccgc
     5821 gtgagcgagg gggagccgtt cggtcgtgcg cacgagccgc tcgccgagct cctccacgcg
     5881 cggcccggct ggcggaccga tcgctcggtc cggacgctcg cgtggcgctt ctcccgcccc
     5941 ggctacgcct atcggacgtt ccagctcctc gactggcgcg ggacctcgcg cgggtacgcc
     6001 gtcgtcgcga tccgcgaggg gcgcgccgcc ctcgtcgacc tgcaggtcgc ttccgagtcc
     6061 gacggcacgc tcgtcgacct gctcgcggcg gtccaggagc ggctcggggc gatcgcggcg
     6121 gaccggatgg tccttcgctc cccccagcga gggctcctcg cgtcccgtct cgagggggag
     6181 ctcgcgttcg tgccggagcc gtcggacacc gtcctcctga tgcatcccat cacggacggg
     6241 tacgacttcc tctccccggg cgcgacgctc gactaccgct tcgccgacca cgacgtcttc
     6301 tgaggagccg acgagcggcc gtctacgcct ggttgcggca ggcgtgacag atcggcagct
     6361 cctcgaagcg cccctcgcgg tggatccggc gcaagcgggc gagggccggc ccctcccaga
     6421 tcccgcggac cgtgtcccct ttcgcgaggt cgccgacgag cagctcgtcc tcgctgtcgt
     6481 ggcagcacgc ggagaccttc ccgtcggcgt ggacggagag gttcatccag agcatccggc
     6541 acgggggacg gcgcatcgtc gcggcggccg gctgcggacg cgacgcgtcg ctccggtcct
     6601 cgaccttccc cgcccagtcg ttgaactcgt tgatcgcgat ctcgtccgag gggccgagaa
     6661 gcggccgcca tcgctcgaag aaggcggcga tctgggccgc ggtctccttc atccggatga
     6721 tccggaggac gaggcgcggc cgcggcgccg ggccgcgcgc cgtgaggagc cgccggacgt
     6781 tcgcctcgac gacgccgagg tcccctccga cccggacggc cgcgtaggtc tccggctcga
     6841 cgccatcgat cgagacgacc accgcgtcga gatcgaggtc gagcagccgc gccacgttcc
     6901 gctcgtcgag gagcgtcccg ttcgtcagga cgacgatcgg cccgatcccc ttcgccttcg
     6961 cgtgggcgag gagctccgcc cacttcgggt ggagcatcgg ctctccgaat ccctgcggga
     7021 agaggacgaa gcccgtctcc gaggcgatct cgtccacgct cttccggaag agcgcctcgt
     7081 ccatgaggcg ccgcttccgg ctcgtcgtcg gcacggggca catgacgcac gcgaggttgc
     7141 agacggcggc cagctcgagc gcgaactcga ccgggaaggc gatcccctcc cagcggtccg
     7201 ggtcgtcgga ggcgagccgg gcgcgccgct cggcgtgggc ctccgccgcc tcgtcccgga
     7261 agagcgcgcg gacctcgagc tccgcgccct gcgggacggc gtcgctcatc gcgcggtccc
     7321 ggaggagagg acgggcagtg ccggggtcgc gtcctcgtag cccatcttcc ccagctggtg
     7381 gatgtcgtag cagtcgcaga agctcggtgt cgtgcagcgg aagggggccg ctcgccgcgc
     7441 gagccggcgt tccttcacgt tcccgaggtc cgcgaggccg ggaatccagg ggcagccctg
     7501 cacgcgcccg tcgttccaga tccggaagta gtcgcgcccg gccgggcaga ggaccgcctc
     7561 cttcccggcg aacggggccg agaagtcgtc cttgtgctcc ttctcgacga cggcggcgta
     7621 tcgctcgagg aggctcgaga agagctcctc ctgcgccgcg tcgaacggcc ggtcgcagtc
     7681 ccggatcgcc cagaccttcc ggccggtcgc cgcgaagacc gtgtcgcggt aatacgcgag
     7741 cccctcctcc cagaggtcgg agaggagcgg cgagaagatc gtcccgagga tcagctcgcc
     7801 ccggtgcttc tcgaggatcg tccgcgcgtt cgcctcccag gccttctcga cgcgcttgtc
     7861 ccgcacctgc ttgtagtgga gcgtcaggtt gaccgcgcgg accttcgcga gcttctcctc
     7921 ggaaagctcc gggtactcgt tcaggaggac gccgttcacg tcgagcgcca cctcgacgtc
     7981 gtggtcgagg agccggtcga ggaaacggtc ccgcttcggg tagccggcga agaagtcccc
     8041 tttgccggag aagatgaaga acgtccgcgt cccgacctcg tcgaggaaga agcgcaggtc
     8101 ctcgtagacg agggacggct cccgtttgac gacgggatgg atgatgcagt agtcgcagtc
     8161 gagctggcag tggcccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     8281 gaacgtcccg tcctgcgggc gctccccgct ccgcggcgag aaggcgtcgg cgtcgaacgg
     8341 ctgggggatg tggatcgctt cctgcccgta gagctccctc acgaggtccg cgaggtgtgg
     8401 cgagacgacg agcttcgtcg ccgggaggcg atagacctcc tcgatctcgg gccagagggg
     8461 acgcgccggc tcgtagatcg cctcgtaggc ctggcagagg tggaacgctc gcgctcctgg
     8521 tacggccatc gcctgcggga cggtgtggta catcgtgccg accgcgacgt cggccggccc
     8581 gatcgcctcc ggcgtcaggt cggggacctg ccggtagaac gaccacgact ccggcgccca
     8641 gtcgctcggc ggcgtcgtgc tgacgacctc ggcgtcgacc ccgaggcggc gcaggatcgc
     8701 gacgtgctgg tagacgacct tcaccccgcc gcagagcgcg gtgccgttga ggaggtagac
     8761 gaccttcatc cgagcgtctc ccagccgacc gggaccgtca tgaagccgct cgtccagcgg
     8821 tccgactcca cccggaaccg aacgtcgctt cgcgcgtcgt agagggcgag gcccttctcg
     8881 tcgccgagga agatgacgag gatgaactcc cccttcgcga gcgggaggga ctccaccgac
     8941 agccggatct cgtggcgcgc cttcccgacg aagggccccg tgccggagac cttcgagtcg
     9001 gcgacgaagc aggtgacctg gtctggcgtg acgacggcga cgtggacgtg gaggggccgg
     9061 tccggcgtgt ccccctcgaa cgcgagctcc aggacccagg ggtcccccgg cgcgaaggcg
     9121 gccgtctcct tcccggcccc gtcgaggagc cggatcgacg tgaaccggcc gagcgagtcc
     9181 tggagcgccg ccggggccgc cacggtccgc tcctgctcct tgcgcgcgag gtactcctcg
     9241 tactggagga cgaccttctg ggcctcgccg agcgcggcga cctccccgtc ccgaagccag
     9301 atcgccgtct cgcagagcgt ggagacgtag tagagggcgt ggctgcagag gaagatcgtc
     9361 cggccgcgcg agcggaggtc gtggaggcgg tcgacgcact tcttctggaa gcgcccgtcg
     9421 cccacggcga gagcctcgtc gaggatgacg acgtcgggga ggaccgcggt cgcgaccgcg
     9481 aacgcgaggc gcatcgtcat ccccgaggag taggtcctca cgggccggtc gaagaagtcg
     9541 ccgagctccg cgaaggcttc gatttccggg agggccgccg cgatctcggc cttcgtgagg
     9601 ccggcgagcg cggcctgcag gaccgcgttc cggcggccgg tcacctccgg gtggaacgag
     9661 gagccgagct ccaggatcgc ggcgacgcgc ccggggagat ggaccgtccc ggaggtcggc
     9721 tccgtcgtcc ccgcgaggat cttcaggagc gtcgacttcc cggagccgtt ctcgccgatg
     9781 accccgagga ccgagccgcg gaggacggtc gcggagacgc cccggagcgc gtggaccgga
     9841 gtgtgccgcg ggcgccccgt gatcgcctcg aggaggcggt cgcgggggga ggcgtagagc
     9901 cggtagacct tcgaaagccc ctcgaaggcg aaggcggcgt cactcacctt cggtccgggc
     9961 ggcgatgcac gaacggcaga tgaagtcgtc cggggcctcc tcgacgccgt acgaccaccg
    10021 ccgcagcagg gcgagggccg gcccctccag gacctcgcgg acgctctgcg tcttcaggtc
    10081 gcccacgacg agcttctcgt cgtagtcctc gcagcagaag atgcacttcc cctccgccgt
    10141 gacgtggagg tgctccagcg gccgcgagcc gacgttgtcg catccccgga gcttccggtg
    10201 cttctcggca ggccggactc cgttcggcag ccaccccgcg cggtcccggg cctcgaacgg
    10261 ccggacgtgg aagcggctcc cgccgtaccg ctcggtgatc tcccggtggg ccttccggtg
    10321 cggttcgtcc cccccgccga ggacgacgag gtccatctcc tctgcgaccg gcttgtcgca
    10381 catgtagtcg atgttgcgga caacagtctc gagctggtcg acgccccgat cccgggcgta
    10441 gcggtccttc tcgagcgtcg agatgttgac cgagaggtag cgcaggccgc cgaggccgag
    10501 gagcgcgtcg acccggtccg gcgtcaggcc ggagccgttc gagaggacgg ccggcggaag
    10561 gcccgccgcc ttgatcgtcg ccacctgctg gacgaaccgg cggtcgaccg tcggctcgtt
    10621 gtagttgttc atgcagacgc cctcgatcga gtcgcggaag gcggagagct ccgcgacgat
    10681 ccgctcgtag agctcggtcg gcatgaagct cttctcgcgg ggggcgaccg aaaccgggca
    10741 gaagtagcag gcctggttgc agacggagtg cgcctcgaga gagacgacct tgaggcggaa
    10801 ccggtgggaa aggtcctcgc cgtccgggat cgcccagagc ccggcccgaa gctcccgctc
    10861 gatcgcgccg tcgacagggc ggccgccccg gagctcgtgg agcggggcga agaggcggtc
    10921 cgtcggggtc agggtcacgt tcgtgagcgg gttgacgacc tgccagccgt cgaagtggag
    10981 gtaggggctc gccaccaggc gaggggcgcc ggagaggggc gcggaggatc gggagacgtc
    11041 gtccggggag gaggcgttca ttcggacgga ttatccacgt gcctccgctc ccgcccggca
    11101 acgcccccgg ccccgccgct ccgcgtcgtt gcgataccct cccgccgtgc cgccccggac
    11161 cgtccccgcc cggccgcgcc ccgcccgcgg agcgctcctc gccgtcgccg cggcgcttct
    11221 cctttcggcc gacgaccggc acgcggggaa ggtctccgac ggccgccaga cgatcggcac
    11281 ggcggtcgcg atcgtcgaga cgggcgagct cgggatcggg aagggagccc cgcgcgcgtt
    11341 cgggcgcgag gccgacgccg tctcgcgcta cggcctcggg gcgagcctcg cccagctccc
    11401 ggcggcggcc ttcgcccccg ccgtggagag cgcgttcggg cccggcacct cgcagccgct
    11461 cttcctcctc gcgccgctcg cgggggtcct cctcgcggcg gccgccgcag gcgg
//
LOCUS       DAOONL010000103        11409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6362_length_11409_cov_15.734014, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000103 DAOONL010000000
VERSION     DAOONL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6362_length_11409_cov_15.734014"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..3031
                     /locus_tag="PLP50_13100"
     CDS             107..3031
                     /locus_tag="PLP50_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA52528.1"
                     /translation="MSLATTLALVASPARALDPSGAPRGFAKTIFREELPQASVLALL
                     QSAEGYLWLGTYEGLVRFDGVEFQVWDRQTVPAMRTNVAQCLAEDASGGIWFGTSRGG
                     VGRLKDGVVEVFGRESGLPSDDVTSVFRDRSGTVWIGTDRGVARWSSGRFEAFGADHG
                     FGRGAVLALAESSDGSLWAGGPGLRVFREGRWSDMEVPAGVELPITALAADQDGTLWI
                     GSISGVTRMGRDGIRTFRIGDGLAGGWVRCLLRDRSGTTWAGTEGRGLFRFRGEKVET
                     FSPVVEEKATDFVISLLEDREGILWIGTRGGLIRMREGVFTSLGAEQGLVGELARAVF
                     QDSSGRVWIGTDGGGLHLVEGGRCTPMNARFRIGSRRVRTIGEEPGGALWVGTSGEGV
                     FRVRDGRTERIGRSEGLADEDVRMVARTRDGTMWVGTRTGLTAFRGGRVLPSDDLTAR
                     AGGTNALLEARDGSLWVGTAGRGLLVSRAGAIATFTTANGLAGDTVFALHEDAEGTIW
                     VGTADGMSHVRGGTATTLRAPEALFRDQVFSILEDAVGGLWMSNNRGVYRFPAGSLAA
                     ADETSQGPFGRLSFGVGDGMPSRQCNGTTQPSALRTRDGRLWYPTANGVAIADPELLR
                     PSAPPPPVVIERALVDGTAVPISGGVVVRPGARRLEIDFAAPDLSAAGRLTYKHRLVG
                     FDDRWSVTRRRFADFTSLPPGRYGFEVVAVGDAGLESTRAARVEVQVPPRLAQTVAFR
                     VGAVLAAVLLAYSAHRLRIRRMAAQERLLNRLVTEKTEALAEANARLEELSLSDSLTG
                     IANRRHFDRRLEEEWRRCRRFGLPLAEVLLDVDLFKAYNDALGHPAGDECLRRVAGAL
                     FGRLRRAGDFVARYGGEEFVALLPGLSLADAYVVAEHLRTQVEALGIPHPAGADAVVT
                     VSAGVASVVPGHGSASRLTSAADAALYEAKRRGRNRSVAASRDRATEVVADRPDTPPG
                     CA"
     gene            complement(3077..3577)
                     /locus_tag="PLP50_13105"
     CDS             complement(3077..3577)
                     /locus_tag="PLP50_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPA52529.1"
                     /translation="MRNGTSHKRLAAGATVLGLLLAAGAAMAGPAGRRGGAAGDLRGA
                     LAALDLSDDQRVKLKDVLQEERERREALRREAWDARKALRTATEAPSPDAAEVGRAYL
                     RLRESREANAEAREKTRERIASILTDEQRAKLDELRSRRRGPQEGRPGRPGRDRGLRG
                     PRPPVR"
     gene            3759..4397
                     /locus_tag="PLP50_13110"
     CDS             3759..4397
                     /locus_tag="PLP50_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52530.1"
                     /translation="MTRAKRWIALLAFTAFAGSAFAAEKAAPVKRLGKTVVRHQDDAV
                     KVVLSWRFANQTFEKEPWLLLELAFAAEGKAVNLNREDVALLGPDGERIPLPGQKRFS
                     EGVRDPLALVKRASVARDPLDNYFSRPTQLESLRFFTVPGGSVVQDEVSGGPSWLIRG
                     DLYFEAPTGVWKPGTYTLVLKNKTMDVALPFSLPAGELKKDAKGADEKVVPW"
     gene            complement(4411..5265)
                     /locus_tag="PLP50_13115"
     CDS             complement(4411..5265)
                     /locus_tag="PLP50_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPA52531.1"
                     /translation="MTRLVLNADDAGLAPGLSRRIASLLASGRLGGTSLLACGEAFGE
                     AVRALGEAGIADAGVHLCLVGGETPLSPPERVRGIAPGGRFPRSWPAVAARVASGLAP
                     VAEVEREWEAQVVAVLSTGLRVTHLDAHQHLHLLPRLAPVAVALARRFGVPYVRAPRG
                     DDPAATADPGLAGSARARLLALFGANARRLLARAGLPEPPRVLGLAEAGRMDLARWRT
                     LLDALPSDGTFEVVLHPGVEDARARERYPWGYSWEEEAAALEDDGLSGMMSARGVARV
                     GFAELSRA"
     gene            complement(5262..5990)
                     /locus_tag="PLP50_13120"
     CDS             complement(5262..5990)
                     /locus_tag="PLP50_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA52532.1"
                     /translation="MRPRRSVSLVVPMRNEERSLPHLLRVALESLARNADEWEIVLVD
                     DASTDGTLALAEEAAAAEPRVRVVRHPVNRGLGGALKSGFAAATMEWVLYSDCDLPWD
                     LEETGRVFRAAELTGADVVSGYRHDRTGEGTLRTVYSFLYNGLVHLAFGFPIRDVNFS
                     CKLVRRSLLAGLPLESDGSFIDAELLVRLRNRGAVIQQVGLDYFPRTRGVSTLSSPRT
                     ILRILRDLARLVPTLRAERRRLAP"
     gene            complement(5993..7153)
                     /locus_tag="PLP50_13125"
     CDS             complement(5993..7153)
                     /locus_tag="PLP50_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52533.1"
                     /translation="MSRPTFPWPWHGPENRKLTLVVLLWLAALQLWALVSIHLLPLGR
                     PSPWTEETTRRAPAFARFDSGWYNSIVEKGYPAPPAPGTQSEHAFFPLYPMTARAIHL
                     ATGLDSFRAAVAVSWAALLLAVPLLASEARERFGERADLRTLPFLLLYPVAFFLAAVY
                     TESLFLLLALLAFRATRRGELAPALLFGLLLGLTRAPAAAVGPGLAVAWWLGGATGTR
                     RAAGASLLLLAPLSGVLAWVFGIGLLQGEPNLFFRSMGAWRTSAGNPFAGAVAFALEP
                     VRMIRTGWFMEHPGAIAPFVHFGLFAVLGAFQLRMRRWADAAWTAGVLGLAVATGTAD
                     GIPRYTLTVYPGHFAAAALCEGRPRLKALWLGASLALLLLNSSFFVNWHFVS"
     gene            7199..7873
                     /locus_tag="PLP50_13130"
     CDS             7199..7873
                     /locus_tag="PLP50_13130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA52534.1"
                     /translation="MTGPSTPRIPGPHEVFRAASALPLGARLHLAVRRWSAPWERVVD
                     ALDGEAALLDVGCGPGLLAFLLESWGAPRPYVGLDPDARKISRARAWLGESDRRTFRA
                     CGLSEAGAATFGAASIVDVLYLVPRAERAGFVAAVAARLAPGGRLVVLTSGGGPRWKR
                     RLDRVQERLAVALLGWTRGAAVEPCDGSEVAALLVAADLAEVRVEDAGAGYLHGFELV
                     SGRKRE"
     gene            complement(7851..10907)
                     /locus_tag="PLP50_13135"
     CDS             complement(7851..10907)
                     /locus_tag="PLP50_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA52535.1"
                     /translation="MRFPRAVGALLAVVALVPASRPASAGTAKLRVGEGVAERVAAEG
                     RVRVLVALDGAPDSPAPEGLETLRSLDRGRLLAGWLTASGLERLGRDPSARAVVLDRV
                     VRPAGQVGTAQIGADRLLSAGLTGIGRAIGIVDTGIDNFHPDFGAGSSGPGRILGGWN
                     FADGNDQTQDCNGHGTAVAGVAAGQQGIAPGASIVALKVFGERDGCSAALASDVLAAV
                     DWALERRSALGLEVLNLSLADGRVRAGFCDDEDPVSARLFARARAEGLIVVASSGNAG
                     FASSMSWPACHSDVVSVGMVYAAGQGPHAWDGDAECTDTYTGPDVVPCTSNGGPALSL
                     LAPGVRWLAPTSGGGRFTTFSGTSAAAPAAAGALLLTRQFTDFDDPALFTDFLRLTGV
                     PVSDGRSALATPRLDVGTALVSTSPASGPCERTEAAGARPAAMVCRTVVSSLTGNVSS
                     LAVALSLEHPRLATVRATLTGPDGTTVSLADGIRRETNVFREVFGRTIDSTDPLSLFA
                     GRPVTGAWTLRLEDGGGFLDGRVTSWALVAEPEAPRLVRLDTVPSRFLPSVARNTGRF
                     GSFFSSDVVLFNPHNEPADVTLSFLSSGSAPEHGVAVDVRLGPRATRTLTDVVGNAFR
                     SSGYGPVRVDGPDHVVVSSRTRTTAPGGGTYGLFVPPMPEANAAGVSDPSTYLAPVFS
                     PDGARVNVGLAEVSGGEANIEIMVRDGRGSIKARYALTLGPFESRQVSDIHAAASAAP
                     AADDLFEVRILSGPGRVASWAIAVDNGSNDGLLVGASRPRRDAFLPAAARSPGKFGAY
                     FRTDLKLSNPWTSPTSVRFSFFPSRGAAPRQLVLSLGAFETRLFADVLGSLLGLPEDS
                     AGALRITALGEGPGLIASSRTYTEEPGKSYGLAIGVLDDAEAEPGDEAALTFLSGSLR
                     TRTNLGFLETRGLETVAEVTLFDAAGSILGTRELTLQPFQAVQWNDVFAQMDVPPSEE
                     ASATVNVARGGALLVHATRVDNATNDGSFLPARVARSRPITPASGR"
     gene            11006..>11409
                     /locus_tag="PLP50_13140"
     CDS             11006..>11409
                     /locus_tag="PLP50_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPA52536.1"
                     /translation="MVPYPEPRKVAEGGVLVVADAHLGQAEGDGRDFLSALEDGVARG
                     YRTVVLLGDIFHYFIGSAKLETPLIREVLEAWDDLASRGVELRYVEGNRDFFLRGTRW
                     ARAFSAYGDTDGLEVGGRRYAFVHGDRVNTRDL"
BASE COUNT         1578 a         3870 c         4479 g         1482 t
ORIGIN      
        1 tcccgtgtcc cccggcggtg gtcgtcggag agggagaggg aagcgagcga tgcgcacgcg
       61 gacgagccgg ggcggagcgg tgcgatcccg gagaaggctc gtgtgggtga gcctggcgac
      121 gacgctggct ctggtcgcct cgcccgcccg ggcgctcgac ccgtccgggg cgccgcgagg
      181 gttcgcgaag accatcttcc gggaggagct tccgcaggct tccgtcctcg ccctcctcca
      241 gtccgccgag ggctatctct ggctgggaac ctacgagggg cttgtccggt tcgacggcgt
      301 ggagttccag gtctgggacc gccagacggt ccccgcgatg cggacgaacg tcgcccagtg
      361 cctcgcggaa gacgcgtcgg gcgggatctg gttcgggacg agccgggggg gcgtgggccg
      421 cctgaaggac ggcgtggtcg aggtcttcgg gcgagagtcg ggcctgccgt ccgacgacgt
      481 gacgagcgtc ttccgagaca ggtccgggac tgtctggatc ggcacggatc ggggcgtggc
      541 ccgctggtcg agcggacgct tcgaagcgtt cggcgcggat cacggattcg gtcgcggggc
      601 cgtcctcgcc ctggcggagt cctccgacgg gagcctctgg gccggcggcc ccggcctgcg
      661 ggtctttcgg gaaggccgat ggagcgacat ggaggttccc gcgggcgtcg agctcccgat
      721 caccgcgctc gccgccgatc aggacgggac cctctggatc ggctcgatct ccggcgtgac
      781 ccgcatgggc cgcgacggga tccggacctt ccggatcggc gacggtctcg cgggcggctg
      841 ggtccgctgt ctcctgcggg accgctccgg aacgacgtgg gccggaacgg aagggcgcgg
      901 gctcttccgg ttccgtgggg agaaggtcga gaccttctcg cccgtcgtgg aggagaaggc
      961 gacggacttc gtcatctcgc ttctcgagga ccgggaaggg attctctgga tcgggacgcg
     1021 tggcgggctc atccgaatgc gcgagggggt cttcacgtcg ctcggggccg agcaggggct
     1081 cgtgggtgag ctcgctcgcg ccgttttcca ggactcctcc ggtcgcgtct ggatcggcac
     1141 ggacggtggc gggctgcacc tcgtcgaggg cggccgctgc acgccgatga acgcccggtt
     1201 tcggatcggg agccgccgcg tccggacgat cggcgaggag cccggtggcg cgctctgggt
     1261 cgggacctcc ggagaagggg tcttccgggt ccgggacggg cggacggagc ggatcggccg
     1321 gagcgagggg ctcgctgacg aggacgtccg gatggtcgcc cggacgcgcg acgggacgat
     1381 gtgggtggga acccggacgg ggctcacagc gttccggggc ggccgtgtgc tgccttccga
     1441 cgacctgacg gcgcgagccg gcggcacgaa tgcgttgctg gaggctcgag acgggagcct
     1501 ctgggtcggg acggccggtc gaggcctcct cgtgtcgcgg gcgggggcga tcgcgacgtt
     1561 cacgaccgcg aacgggctcg cgggtgacac ggtcttcgcg ctgcacgagg acgcggaggg
     1621 aacgatttgg gtcggaacgg cggatggaat gagccacgtg aggggaggga cggcgacgac
     1681 gctgcgggcg ccggaggcgc tgttccggga tcaggtcttc tcgatcctcg aggacgcggt
     1741 cggcgggctc tggatgagca acaacagggg cgtctacagg ttcccggccg gaagcctcgc
     1801 ggccgccgac gagacgtcgc aagggccctt cggtcggctc tccttcggag tcggcgacgg
     1861 catgccgagc cgtcagtgca acgggacgac gcagccctcg gccctccgca cgcgggacgg
     1921 acgcctctgg tacccgacgg cgaacggcgt ggcgatcgcc gacccggagc tccttcgtcc
     1981 gagcgcgccg cctcctccgg tcgtcatcga gcgggcgctc gtcgacggga ccgcggttcc
     2041 gatctccggg ggcgtcgtcg tcaggcccgg cgcgcggagg ctcgagatcg acttcgcggc
     2101 gcccgacctg agcgccgccg gacggctcac ctacaagcac cggctcgtcg gcttcgacga
     2161 ccgctggagc gtcacgcgcc gccggttcgc ggacttcacg tcgctccctc ccggccgcta
     2221 cggcttcgag gtcgtggccg tcggggacgc cgggctcgag agcacgcggg cggcgagggt
     2281 cgaggttcag gtcccgccgc gtctcgcgca gaccgtcgcg ttccgggtcg gggccgtcct
     2341 ggcggcggtc cttctcgcct attccgctca ccgcctccgc atccgccgga tggcggcgca
     2401 ggagcgcctg ctgaaccggc tcgtcacgga gaagacggag gcgctcgccg aggcgaacgc
     2461 ccggctcgag gagctgtcgc tctccgactc gctcacgggg atcgcgaacc ggcggcactt
     2521 cgaccgccgg ctcgaggagg agtggcggcg ctgccggagg ttcggtctcc cgctggcgga
     2581 ggtcctcctc gacgtcgacc tcttcaaggc ttacaacgac gcgctcggcc acccggccgg
     2641 cgacgagtgc ctgcgtcgcg tcgccggtgc gctcttcggc cggctccgcc gggccgggga
     2701 cttcgtcgcg cgatatggcg gcgaggagtt cgtcgcgctc ctgccgggcc tgtctctcgc
     2761 ggacgcatac gtcgtcgccg agcatctgcg gacgcaggtc gaggcgctcg ggatcccgca
     2821 tccggccggt gcggacgccg tcgtgacggt cagcgccggg gttgcgtccg tcgttccggg
     2881 acacggctcg gcgtcccggc tgacctccgc ggcggatgcc gccctctacg aggcgaagag
     2941 gaggggacga aaccggtccg tggcggcgag tcgggaccgg gcgacggagg tcgtcgcgga
     3001 tcggccggac accccgcccg gttgcgcctg aaccgagcgg ggcgggagcg cggccggacg
     3061 aaccggccgg gtcgggtcaa cggacgggag gccgtggacc gcggagtccg cgatcccgtc
     3121 cggggcggcc cgggcgcccc tcctgcgggc ctctccggcg cgaacggagc tcgtcgagct
     3181 tcgcccgctg ctcgtcggtc aggatcgacg cgatccgctc tctcgtcttc tcgcgggctt
     3241 cggcgttcgc ttcgcggctc tcccgaaggc gcaggtaggc gcgtcccacc tcggcggcgt
     3301 ccggggaggg ggcttcggtc gccgtgcgaa gggcctttct cgcgtcccag gcctcgcgcc
     3361 gaagggcctc gcggcgctct cgctcttcct ggaggacgtc cttcagcttc acgcgctggt
     3421 cgtcggagag gtcgagggcc gcgagggcgc ctcggaggtc gccggcggcg ccgccccggc
     3481 gtcccgccgg cccggccatc gccgcgccgg ccgcgaggag gagtcccaga accgttgcgc
     3541 ccgcggcgag tctcttgtgt gacgttccgt ttctcatcgc tgctccttcc gcgtccggga
     3601 gccgtcggtc gaccgggccg gacaaaccaa gagacgcggt aggggcgcat cccgttgacg
     3661 ccgtcgtccg acgtgctggc gcgagatgtg ctagatgtcg tctcattccg tcccgccgcg
     3721 caggatcgac ctcgcgcggg gacggtgagg aggttccgat gacccgcgcc aagcgttgga
     3781 ttgccctgct cgccttcacg gccttcgccg gctccgcctt cgccgcggag aaggcggccc
     3841 ccgtcaagcg cctcgggaag accgtcgtcc gccaccagga cgacgcggtg aaggtcgtcc
     3901 tctcgtggcg attcgccaac cagaccttcg agaaggagcc atggctcctc ctggagctcg
     3961 cgttcgccgc ggaggggaag gccgtcaacc tgaaccggga agacgtcgcg ctcctcgggc
     4021 cggacggaga gcggatcccg ctgccgggcc agaagcgctt ctcggaggga gtccgggacc
     4081 cgctcgccct cgtcaagcgg gcgtcggtcg ctcgagaccc gctggacaac tacttctcca
     4141 ggcccacgca gctcgagagc ctgcgcttct tcacggtgcc gggcggatcg gtcgtgcagg
     4201 acgaggtctc cggcggcccg agctggctga tccgggggga cctctacttc gaggcgccca
     4261 ccggcgtctg gaagccgggg acctacacgc tcgtcctgaa gaacaagacg atggacgtcg
     4321 cgctgccgtt ctcgctgccg gccggcgagc tgaagaagga cgcgaagggg gccgacgaaa
     4381 aggtcgtccc ctggtgacgc gggcgggcgt tcaggcgcgc gagagctccg cgaagccgac
     4441 gcgcgccacg ccccgcgccg acatcattcc ggagaggccg tcgtcttcga gggcggcggc
     4501 ctcttcttcc cacgaatagc cccacgggta gcgctcgcgc gcccgggcgt cctcgacgcc
     4561 ggggtggagg acgacctcga acgtcccgtc ggacggcagc gcgtcgagga gcgttcgcca
     4621 gcgcgcgagg tccatgcgtc ccgcctccgc gaggccgagg acgcgcggag gctcggggag
     4681 gccggcccgc gcgaggagtc tccgcgcgtt cgcgccgaag agcgcgagga gcctcgcccg
     4741 ggcgcttccc gcgaggccgg gatcggccgt cgcggcgggg tcgtccccgc ggggcgcccg
     4801 gacgtacggg acgccgaagc gccgcgcgag cgcgacggcg accggagcga gtcgcggaag
     4861 gaggtggagg tgctggtgcg cgtcgaggtg cgtcacccgc aggcccgtcg agaggacggc
     4921 gacgacctgg gcctcccatt cccgctccac ctccgcgacc ggggcgaggc cggaggcgac
     4981 ccgcgccgcg accgccggcc atgagcgcgg gaagcgccct cccggggcga tccctcgaac
     5041 gcgctccgga ggggagagcg gcgtctcgcc gccgacgagg cagaggtgga cgcccgcgtc
     5101 ggcgatgccc gcttcgccca gcgcgcgaac cgcctcgccg aacgcctcgc cgcacgcgag
     5161 gaggctcgtc ccgccgaggc gcccggaggc gaggagcgag gcgatccgcc gcgagaggcc
     5221 gggcgcgagg ccggcgtcgt ccgcgttcag gacgagccgc gtcacggcgc gagccgacgg
     5281 cgctcggccc ggagcgtcgg gacgaggcgg gcgaggtcgc gaaggatgcg gaggatcgtc
     5341 cgcggcgagg agagcgtgga gacgccgcgc gtccggggga aatagtcgag gccgacctgc
     5401 tggatgacgg ccccgcggtt gcggaggcgg acgagcagct cggcgtcgat gaaggagccg
     5461 tcgctctcga gggggagtcc cgcgaggagc gagcgccgga cgagcttgca ggagaagttc
     5521 acgtcgcgga tcgggaagcc gaacgcgagg tggacgagcc cgttgtagag gaaggagtag
     5581 accgtccgga gcgtcccctc gccggtccgg tcgtgacggt agcccgagac gacgtcggcg
     5641 cccgtcagct cggcggcgcg gaagacgcgc cccgtctcct cgaggtccca ggggaggtcg
     5701 cagtcggagt agaggaccca ctccatcgtc gcggccgcga agccggactt cagggcgccg
     5761 cccagccccc ggttcacggg atggcgcacg acgcggacgc gcggttccgc ggcggcggcc
     5821 tcctcggcga gcgcgagggt cccgtctgtc gaggcgtcgt cgacgaggac gatctcccac
     5881 tcgtccgcgt tccgcgcgag agattcgagg gcgacgcgca ggaggtgggg gaggctccgc
     5941 tcctcgtttc gcatcgggac gacgagggag accgatcggc gcggccgcat cgtcacgaca
     6001 cgaagtgcca gttcacgaag aacgaggagt tgaggaggag gagggcgagg gacgcaccga
     6061 gccagagggc cttcagccgg ggccggccct cgcagagcgc ggcggcggcg aagtgcccgg
     6121 ggtagacggt gagcgtgtag cgcggaatgc cgtcggcggt cccggtcgcc acggcgaggc
     6181 ccaggacccc cgccgtccac gcggcgtcgg cccagcggcg catccggagc tggaacgctc
     6241 cgaggaccgc gaagaggccg aagtggacga acggcgcgat cgctcccggg tgctccatga
     6301 accagccggt ccgaatcatc cggaccggct ccagggcgaa cgcgacggcc ccggcgaaag
     6361 ggttgccggc ggaggtccgc cacgcgccca tcgaccggaa gaagaggttc ggctctccct
     6421 gcagcaggcc gatcccgaag acccacgcga ggaccccgga gagcggcgcc aggaggagga
     6481 gagaggcacc ggccgcccgt cgcgtcccgg tcgccccgcc cagccaccag gcgaccgcca
     6541 ggcccggccc gacggcggcg gccggcgccc gggtgagtcc gagcaggagg ccgaagagga
     6601 gcgcgggagc gagctcgccc cgccgggtcg cgcggaaggc gaggagcgcg aggaggagga
     6661 agagcgactc ggtgtagacc gccgcgagga agaaggcgac ggggtagagg aggaggaacg
     6721 gcagcgtccg gaggtccgcc cgctcgccga agcgctcgcg ggcctccgag gccaggagcg
     6781 gcacggcgag gaggagcgcg gcccagctga cggcgacggc ggcccggaac gaatcgagcc
     6841 ccgtcgcgag gtgaatcgcg cgggcggtca tcgggtagag gggaaagaag gcgtgctccg
     6901 actgggttcc cggggcgggc ggagccggat agcccttctc gacgatcgag ttgtaccagc
     6961 ccgagtcgaa gcgggcgaag gccggcgcgc gtcgcgtcgt ctcctcggtc cacggagagg
     7021 gccgaccgag cggaaggaga tggatcgaga cgagcgccca gagctgaagc gcggccagcc
     7081 agagaaggac gacgagggtc agcttccggt tctccgggcc gtgccacggc caggggaagg
     7141 tcggacgact catctcggga ggcctcgcac cgcgggatcg gagattactc tatggtccgt
     7201 gaccggcccg tcgacgccca ggatccccgg accgcacgaa gtcttccgcg cggcgtccgc
     7261 gctcccgctc ggcgcgcggc tccacctcgc cgttcggagg tggagcgcgc cctgggaacg
     7321 ggtcgtcgac gcgctcgacg gggaggccgc gctcctcgac gtcggctgcg ggcccgggct
     7381 cctcgcgttc cttctcgagt cgtggggagc ccctcgcccc tacgtcggcc tcgaccccga
     7441 cgcgaggaag atctcgcgcg cgcgggcatg gctcggggag tcggatcgcc ggacgttccg
     7501 cgcctgcggg ctctcggagg cgggagccgc gacgttcggt gcggcttcga tcgtcgacgt
     7561 cctctacctc gttccccgcg ccgagcgcgc cggcttcgtc gccgccgtcg cggcgcggct
     7621 cgcgcccggc gggcggctcg tcgtcctgac gagcggcgga ggcccccgct ggaagcgacg
     7681 gctcgaccgg gtccaggagc gcctcgccgt cgccctcctg ggctggaccc gtggcgccgc
     7741 cgtcgagccg tgcgacgggt ccgaggtggc ggcgctcctc gtggcggccg acctcgccga
     7801 ggtccgcgtg gaggacgctg gcgccggcta cctccacggg ttcgagctgg tcagcggccg
     7861 gaagcgggag tgatgggccg ggatcgagcg acgcgggcgg ggaggaagct cccgtcgttc
     7921 gtcgcgttgt cgacgcgtgt ggcgtggacg aggagcgctc ccccgcgcgc gacgttcacg
     7981 gtcgcggagg cctcctcgga aggtgggacg tccatctggg cgaagacgtc gttccactgg
     8041 accgcctgga acggctggag cgtcagctcg cgcgtcccga ggatggagcc cgccgcgtcg
     8101 aagagcgtca cctcggcgac cgtttcgagc ccccgggtct cgaggaaccc gaggttggtc
     8161 cgggtcctca ggctgcccga caggaacgtc agcgccgcct cgtccccggg ctccgcctcg
     8221 gcgtcgtcga ggacgccgat cgccagtccg tagctcttcc cgggctcctc ggtgtaggtc
     8281 cggctggagg cgatcagccc gggcccctcg ccgagcgccg tgatccgaag cgcgcccgcg
     8341 gagtcctcgg ggaggccgag gaggctcccg aggacgtccg cgaagaggcg cgtctcgaac
     8401 gcgccgagcg agaggacgag ctgcctcggg gcggcgccgc gcgacgggaa gaaggagaag
     8461 cgaacgctcg tcggagaggt ccaggggttc gagagcttga ggtccgtccg gaagtaggcc
     8521 ccgaacttcc cgggcgaacg ggccgccgcg ggaaggaagg cgtcccggcg agggcgggag
     8581 gcgccgacga gaagtccgtc gttggagccg ttgtcgacgg cgatcgccca gctcgcgacg
     8641 cggccagggc ccgagaggat gcgcacctcg aagagatcgt cggcggcggg cgcggcggag
     8701 gcggcggcgt ggatgtcgct cacctggcgg ctttcgaacg gtccgagcgt cagggcgtag
     8761 cgcgccttga tcgagccgcg gccgtcgcgg accatgatct cgatgttcgc ctctcctccc
     8821 gagacctcgg cgaggccgac gttgacgcgg gcgccgtcgg ggctgaacac gggggcgagg
     8881 taggtcgacg gatcggagac cccggcggcg ttcgcttccg gcatcggcgg cacgaagagg
     8941 ccgtaggtcc ctccgcccgg ggccgtcgtc cgcgtccggc tcgagaccac cacgtggtcc
     9001 gggccatcga cgcggacggg cccgtacccg gaagagcgga atgcgttgcc cacgacgtcg
     9061 gtcagggtgc gcgtcgcgcg ggggccgagg cggacgtcga cggcgacgcc gtgttctggc
     9121 gcggagccgg aggagaggaa cgagagggtg acgtcggcgg gctcgttgtg gggattgaac
     9181 aggacgacgt cgctggagaa gaacgacccg aatcgtccgg tgttcctcgc gaccgagggg
     9241 aggaaccggg acgggaccgt gtcgaggcgg accaggcggg gcgcttcggg ctccgcgacg
     9301 agtgcccagc tcgtcacccg gccgtcgagg aaccctccgc cgtcctcgag gcggagcgtc
     9361 caggctccgg tgacgggcct gccggcgaag agggagagcg ggtccgtgct gtcgatcgtc
     9421 ctcccgaaga cctccctgaa gacgttcgtc tcgcggcgga tgccgtccgc gagggagacc
     9481 gtcgtgccgt ccggcccggt gagggtcgct cggaccgtgg ccagtctcgg gtgctcgaga
     9541 gagagggcga cggcgagcga cgagacgtta cccgtcaggg aggagacgac ggtccggcag
     9601 accatcgcgg ccggtctcgc tcccgcggcc tcggtcctct cgcaggggcc ggaagcgggg
     9661 ctcgtcgaga cgagcgcggt cccgacgtcg agccggggag tcgcgagcgc ggatcgtccg
     9721 tcgctcacgg gtacgccggt cagccggagg aagtcggtga agagcgcggg gtcgtcgaaa
     9781 tcggtgaact gccgcgtgag gaggagggcg cccgcggcgg cgggcgcggc ggccgaggtc
     9841 ccggagaagg tggtgaaccg tcctccgccc gaggtcgggg cgagccacct cacgccggga
     9901 gcgaggagcg agagggcggg gcctccgttg ctcgtgcagg ggacgacgtc cgggccggtg
     9961 tacgtgtcgg tgcactcggc gtctccgtcc caggcatggg gaccctggcc cgcggcgtag
    10021 accatcccga cgctgacgac gtcggagtga caggcgggcc aggacatcga ggaggcgaag
    10081 ccggcgttgc cggaggaggc gacgacgatg agcccctcgg cgcgcgcccg ggcgaagagg
    10141 cgcgccgaca cggggtcctc gtcgtcgcag aagccggccc ggacccggcc gtcggcgagg
    10201 ctcaggttga ggacctccag gccgagagcg ctgcgccgtt cgagcgccca gtcgacggcc
    10261 gcgaggacgt ccgaagcgag cgccgcggag cagccgtccc tctcgccgaa gaccttcagg
    10321 gcgacgatcg acgccccggg cgcgatcccc tgctgtcccg cggcgacccc ggcgacggcc
    10381 gtcccgtgtc cgttgcagtc ctgggtctgg tcgttcccgt cggcgaagtt ccagccgccg
    10441 aggatccgtc ccggtccgct cgagcccgct ccgaaatcgg ggtggaagtt gtcgatcccg
    10501 gtgtcgacga tcccgatggc gcggccgatc ccggtcaggc cggccgaaag gagccggtcg
    10561 gcgccgatct gagccgtccc gacctgtccg gcggggcgga cgacgcgatc caggacgacg
    10621 gcccgcgcgg agggatcccg gccgaggcgc tcgagcccgg acgcggtcag ccagccggcg
    10681 aggagccggc cgcgatcgag cgaccggagc gtctcgaggc cctccggcgc gggcgagtcg
    10741 ggggcgccat cgagggcgac gaggacacgg actctcccct ctgccgcgac ccgctccgcg
    10801 accccctcgc cgacccggag cttcgccgtc ccggccgagg ccggtcggga ggccgggacg
    10861 agggccacga cggcgaggag cgcgccgacg gcgcgtggga atctcattgc tgctcgcgga
    10921 agctatcacc cggaaccggc ccgcaaggtg agccgcgtca actcatgggc cgagaaggag
    10981 tgagccgggc cgctagaatc ccatcgtggt cccctatccg gagccgcgaa aggtcgcgga
    11041 aggtggtgtg ctcgtcgtcg ccgacgccca tctcgggcag gcggaggggg atgggaggga
    11101 cttcctctcc gcgctggagg acggtgtcgc ccggggatac cggacggtcg tcctgctggg
    11161 agacatcttt cactacttca tcggaagcgc gaagctcgag acgccgctca tccgcgaggt
    11221 cctcgaggcc tgggacgatc tcgcgtcgag aggcgtggag cttcgctacg tcgaggggaa
    11281 ccgggacttc ttcctgcgcg gcacgcggtg ggcgcgggcg ttctccgcgt acggcgacac
    11341 cgacggcctg gaggtcggcg gacgccggta cgccttcgtc cacggcgacc gcgtcaacac
    11401 gcgcgacct
//
LOCUS       DAOONL010000104        11305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6443_length_11305_cov_16.649244, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000104 DAOONL010000000
VERSION     DAOONL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6443_length_11305_cov_16.649244"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1146)
                     /locus_tag="PLP50_13145"
     CDS             complement(58..1146)
                     /locus_tag="PLP50_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPA52537.1"
                     /translation="MRIAVDARSLAGARTGVGRTLEGLYDALLRLFPGDSAVFLSPRS
                     VTLPPLLEGRVEVVPPARPRLPGTLWLQTVAPLAARRAGADLFHGPLGVLPLASALPG
                     VATVHDLTPILFPEWHDRKNVAGFAPLLPGTVRRARALVCVSRSTRDDLLLALPESGG
                     KAHVVPNGLVPPPAGSATAPCRARPYVLSIGTLEPRKNHERLVAAMESIWDRRPDFPD
                     LVLAGGDGWGMPSFRDRLRRSRHAARIEAPGWVSPESAAGLLRSARVFAYPSLYEGFG
                     LPALEAMAEGVPVVASSSSSLPEVVGDAGLLPDPADVRAIAAALERACDDDAFREEAA
                     RRGRDRARLFTWEAAARRTRELFEEALA"
     gene            1268..1780
                     /locus_tag="PLP50_13150"
     CDS             1268..1780
                     /locus_tag="PLP50_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPA52538.1"
                     /translation="MELNLEEALEAPVALSHRLEVPVGRLERPELLSLEPVEFSGTLQ
                     KAEPGFVLSGELAVSGQAVCSRCLAPVPFASRGDVSWVFAPAHRRPTAEETELTATDL
                     EVVWYEDFIVPFDPLVEEMLQLEIPLKPLCRPECKGLCPTCGADRNVAPCDCREEGDE
                     RLAKLKSLLS"
     gene            1847..2032
                     /gene="rpmF"
                     /locus_tag="PLP50_13155"
     CDS             1847..2032
                     /gene="rpmF"
                     /locus_tag="PLP50_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPA52539.1"
                     /translation="MPNPKHRHSKTRRDMRRAHDFLQEPGLSKCASCGATKTPHRVCP
                     ECGHYRGREVSAGAQKS"
     gene            2059..3072
                     /gene="plsX"
                     /locus_tag="PLP50_13160"
     CDS             2059..3072
                     /gene="plsX"
                     /locus_tag="PLP50_13160"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPA52540.1"
                     /translation="MTLAVDAMGGDNAPRATVEGAVAAAVDFGLDLLLVGRRRELEPI
                     LSATSYRGSRIDLVDAEEVVGFDEPSTTALRRKPRASIRVGAELVRDGRAEGFFTAGH
                     TGAAMVTARSVLGVIEGIDRPALGAVLPGLGRRRLLLDVGANVSCRVEHYREFALMGH
                     FYAQEVLGISRPKIGLLSVGEEEEKGTGTTREVFGLLRKSGLNFVGNCEGRDVYGGNV
                     DVDVIVTDGFTGNVVLKASESLARLVEEALKQEVTRSLQASMGFLLSRDAFRSFKKRL
                     DYTEYGGAPLLGVKGAVLIGHGSSNARAVRNGVKALHEFARRRIPEKIRAKARELAPL
                     AAR"
     gene            3089..4072
                     /locus_tag="PLP50_13165"
     CDS             3089..4072
                     /locus_tag="PLP50_13165"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPA52541.1"
                     /translation="MFHAAIAGTGRAVPAKLLTNADLETLVETTDEWITTRTGIRERH
                     QAAEGEVLSDFCVEAAKQALEAAWLQAKDLDAILIATVTPDQPIPSMACLVQQKLGAK
                     KAAAWDQNAGCSGWLYGLHIADGLIQAGKARNVLLVGAEFLTRYVDYTDRATCVLFGD
                     GAGATILRATKSDRGVLASTIRSDGEGACFIQMPGGGSSFPPTRPETLAERLPFIKMM
                     GGETFKLAVRSMVEVCKDVLNESGFEPGEVSWLVPHQANDRIIQAVGERLGIPRERCI
                     VNIERYGNTSAASIPIALDEAARDGRVKRGDLILFTAFGAGLTWGAALVRW"
     gene            4075..5013
                     /gene="fabD"
                     /locus_tag="PLP50_13170"
     CDS             4075..5013
                     /gene="fabD"
                     /locus_tag="PLP50_13170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPA52542.1"
                     /translation="MGSLSFLFPGQGSQVAGMGRDLVNAFPEARAVYERADAALAPFG
                     LAISRVSFEGTDEELKRTAVTQPAILVHSVAVFEVLKARGLTPTMLAGHSLGEWSALV
                     AAGALSLEEAVVLVHRRGTFMQEAVPAGEGAMGAVLGLDGALVAEACAEVESATGEAV
                     SAANFNSPEQTVIAGTTNAVAKAGELLRQKGAKRVLPLPVSAPFHCALMKPAEEKLGP
                     FLDAAPFSDLAVPVVTNVDVLANLSGAEAREALRRQVCSPVRWVETVQRLSRDTEGAI
                     EVGPGTVLAGLAKRIAKDWLVRTTSDAEGIGKLLAG"
     gene            5010..5486
                     /locus_tag="PLP50_13175"
     CDS             5010..5486
                     /locus_tag="PLP50_13175"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HPA52543.1"
                     /translation="MTTKATTNATRVLDARKAVYALRTFAAADFTAEEAAEKLGVPPG
                     EVWKTLVAKGDRTGLLAAVIPTGTRLSLKKLARVSGNRTVAMVSPEEMEKATGYVKGG
                     CSPFGMRKAMPLYVHEAAPAMPRLFVSAGQRGVQLEMAGEAFLAAAGATAADLIEE"
     gene            5495..6253
                     /gene="fabG"
                     /locus_tag="PLP50_13180"
     CDS             5495..6253
                     /gene="fabG"
                     /locus_tag="PLP50_13180"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPA52544.1"
                     /translation="MPHVITLEGKVALVTGASQGIGEAVARRLAAAGATVLVAARSEE
                     KAAAVAASIAEAGGAAESVRLDVSDPASVTAAFKGIVERHGKLDVLVNNAGITDDGLL
                     LRMSKESWDRVIATDLTGVFLVSQEAVKLMMKKRVAGRIITVGSVVGLMGNPGQANYA
                     AAKAGVVGFTMSLAREIGSRGITVNAVAPGYVETAMTGALSEDQRKALAEKIVLGRLG
                     TGEDVAGAVLYLASDLASYVTGTVLNVSGGLLIP"
     gene            6338..6583
                     /gene="acpP"
                     /locus_tag="PLP50_13185"
     CDS             6338..6583
                     /gene="acpP"
                     /locus_tag="PLP50_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPA52545.1"
                     /translation="MSNSIEDKVKSIIVEQLGVQAEEVKAEASFVDDLGADSLDTVEL
                     VMAFEEAFGIEIPDEDAEKIQKVKDAVTYIESHAKPA"
     gene            6602..7858
                     /gene="fabF"
                     /locus_tag="PLP50_13190"
     CDS             6602..7858
                     /gene="fabF"
                     /locus_tag="PLP50_13190"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPA52546.1"
                     /translation="MDHLDARGRRRVVVTGIGLVSPVGLTREENWDALLAGRSGIGAI
                     TRFDAADYACRIAGEVKGFDPALFLDRKEIKKFDTFVHYAVAAAKEALAESGLAITEE
                     NAEAVGVCVGSGIGGLPLIEETHKVLLEKGPRRISPFFIPGLIANMSSGLISILTGAK
                     GPNTATVTACATSAHAIGDAVNIIRRGDADAMISGGTEAVVSPLAVGGFAAMRALSTR
                     NDDPAAASRPWDRDRDGFVLGEGAGILVLEELGHAVRRGATIYAEVAGYGMSGDAHHI
                     SAPSDDGDGPFRVMRNALKDAGMEPSEVGYVNAHGTSTPHGDRIETIALKRLFGEGAR
                     SVPVSSTKSMTGHLLGAAGGYEAGVLALAIRRGVLPPTVNYTTPDPECDLDYTPNVAR
                     EVRVRAGLSNSFGFGGTNACLVMRAV"
     gene            7953..9443
                     /locus_tag="PLP50_13195"
     CDS             7953..9443
                     /locus_tag="PLP50_13195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA52547.1"
                     /translation="MKADQVRRKFFAGVPRYEWIETLGQGGVGIVYKAKDLELEEVVA
                     IKVLSPDIAKEDEVVLARFKREINLNRKIKHPNVARMYDFGISGEYPYITMEVVPGKD
                     LWTMIRDEGRLPPARAVPILRQIARGCSAIHELGIVHRDLKSQNVVVDEQGAVAIVDF
                     GLARWNLGGGFTLDSIILGTPQYMSPEQASGKTVDSRTDIYSIGIIAFEALTGQLPFT
                     GDSPIAVAMMQINDPVPDLLPKFADVSPDLRAIVLKALRKDPAKRYATAADLEADLAN
                     ADRLPTPREGTVPHPGGEDPLMAQLESVLGAVVLPAPPEKPRRPSPSDASAPVTRPSV
                     SPRPAAARPAPTPPAAPEAPSRPPTVLIVASAPAERAAWKAGLGIGPCRVLEAGSGPE
                     ALEALVREPADLVLMDVALPGMDGFDVTRVIKSQANLVALPILLASDRLERSHYAFGI
                     QSGANELLVKPIEPERLREAVRGLLRHRGFPVEDGPAGAGPVAGNG"
     gene            9496..10413
                     /locus_tag="PLP50_13200"
     CDS             9496..10413
                     /locus_tag="PLP50_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52548.1"
                     /translation="MSRDSGPDDPVGEAEAHAEPGRPAEKRAGYWDPTASGRHQISRP
                     IAIDGLRADRARIELAPRVVRRGGTYYDDSYHVVDAAARQLASIFTLEQLVMATVALR
                     VHMRRGREHLGRKRELQERWTVLAHRLMKLRAEERHLALREQRIAEEHASLAGAESER
                     PGKAELLTLEEAEVAQARQAWRDESRRAWEAWSRLESEEGALLAGAPGLEQTEDLKQP
                     YILLLVGARRAAPRSVAAAMHTLVDALQRPLSRTDQMLLEIAFRRCIRAGSPEDERDL
                     ERWEHELLAAAERDFAAEFPWARPYLGNG"
     gene            10472..11278
                     /locus_tag="PLP50_13205"
     CDS             10472..11278
                     /locus_tag="PLP50_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPA52549.1"
                     /translation="MCLIVLALHAHPLYPLVVAANRDEALARPSAPADFWPDADGLLA
                     GRDEESGGTWMGVTRSGRFAALTNVRDGRPPEPLAPSRGGLVVRFLAGGDEPLAHARA
                     VAAERVRRNGFNLLAGAGGRLAWVSNAGGGPAEVTPGVHAVSNAALDSPWPKSLRTAA
                     GLRRVLEDGGAIDPEELFAILSDRRIAADDELPETGVPLEVERLLSAPFIVAPGYGTR
                     SSTLLLAARDGRAELLERRFDTSFRESGTSRFELDFPGWNGRVETRFPSS"
BASE COUNT         1640 a         4010 c         4169 g         1486 t
ORIGIN      
        1 aggtgccgat gccgaagtcg cggagcttgc gcgcgtcgag cgcgacccgc aggcgcttca
       61 agcgagggcc tcctcgaaga gctcccgggt ccgtcgcgcg gcggcctccc acgtgaagag
      121 ccgcgcccgg tcgcgcccgc ggcgcgcggc ctcctcgcgg aacgcgtcgt cgtcgcaggc
      181 ccgctcgagc gcggccgcga tcgcgcggac gtcggccggg tcggggagga ggccggcgtc
      241 gccgacgacc tcgggaagag acgaggacga cgaggcgacg accgggaccc cctcggccat
      301 tgcctccagg gccgggaggc cgaacccctc gtagaggctc gggtaggcga agacgcgcgc
      361 cgagcggagg agccccgcgg cggactccgg cgagacccag ccgggcgcct cgatccgggc
      421 cgcgtggcgc gagcggcgaa ggcggtcgcg gaaggaaggc atcccccacc cgtcccctcc
      481 ggcgaggacg aggtccggga agtcgggccg ccggtcccag atcgactcca tcgcggcgac
      541 gagacgctcg tggttcttcc gcggctcgag ggtgccgatc gagaggacgt aggggcgcgc
      601 gcggcacggc gccgtcgcgg agccggccgg gggagggacg aggccgttcg ggacgacatg
      661 cgcctttccg cccgattccg gaagggcgag gaggaggtcg tcccgggtcg agcgggagac
      721 gcagacgagg gcccgcgccc tccggaccgt tccgggaagg agcggggcga agccggcgac
      781 gttcttccgg tcgtgccact cggggaagag gatcggcgtc aggtcgtgga cggtggcgac
      841 tccgggaagg gcggaggcga ggggcaggac cccgagcggg ccgtggaaga ggtccgcccc
      901 ggcccggcgg gccgcgagcg gggcgaccgt ctggagccag agggttcccg gaaggcgggg
      961 gcgggcgggc gggacgacct cgacccgtcc ctcgaggagg ggggggaggg tgaccgaccg
     1021 gggggagagg aagacggcgg agtcgcccgg gaagaggcgg aggagcgcgt cgtagagccc
     1081 ttcgagcgtc cggccgacgc cggttctcgc cccggcgagc gagcgggcgt cgacggcgat
     1141 gcgcattcag ggcatggtac cgcgcgacca gcgcggtccg ccggtcccgg aggggcgtcg
     1201 tccggagcgg aacggcccgt cggaggcgcg tgctacactc gccgaccctc cacgaggagg
     1261 ggtcgagatg gaactgaatc tggaagaggc gctggaggcg cccgtggctc tttctcaccg
     1321 cctcgaggtc cccgtgggac gcctcgagcg gcccgagctg ctttcgctcg agccggtcga
     1381 gttctcgggc accctccaga aggccgagcc cgggttcgtc ctgagcggcg agctcgccgt
     1441 ctcagggcag gcggtctgct cgcgctgtct cgcgccggtt ccgttcgcga gcagggggga
     1501 cgtctcctgg gtcttcgcgc cggctcaccg gcggccgacc gcggaggaga cggagctcac
     1561 cgcgaccgac ctcgaagtcg tctggtacga ggacttcatc gtcccgttcg acccgctcgt
     1621 cgaagagatg ctccagctgg agatacccct gaagccgctc tgccggcccg agtgcaaggg
     1681 gctctgcccc acctgcggcg ccgaccggaa cgtcgccccg tgcgactgcc gagaagaggg
     1741 tgacgagagg ctcgcgaagc tcaagtcgct cctgtcctag gccgccgaag gcgacgacgc
     1801 cccgatgcgc cccccgacac gaaacgatcc cgagaggtga actgccatgc cgaatccgaa
     1861 gcaccgtcac agcaagaccc gccgcgacat gcggagggcg cacgacttcc tccaggagcc
     1921 gggcctctcc aagtgcgcca gctgcggcgc gaccaagacc ccccaccgcg tctgccccga
     1981 gtgcggccac taccgcggac gggaagtctc cgccggcgcc cagaagagct gatcgcggcc
     2041 gttccggtcg cgtcctgaat gaccctcgcc gtcgacgcca tggggggaga caacgccccc
     2101 cgtgccaccg ttgaaggagc cgtcgcggcc gccgtcgact tcggcctcga ccttctcctt
     2161 gtggggcgcc ggcgcgagct ggagcccatc ctctcggcga cgagctaccg ggggagccgg
     2221 atcgacctcg tcgacgcgga ggaggtcgtc gggttcgacg agccttcgac gacggcgctc
     2281 cgccggaagc cccgcgcctc gatccgcgtc ggagccgagc tcgtccgcga cggccgggcg
     2341 gagggcttct tcacggccgg tcacaccggc gccgcgatgg tcacggcccg ctccgtcctc
     2401 ggcgtgatcg aggggatcga ccgtcccgcc ctcggtgcgg tcctcccggg tctcgggcgc
     2461 aggagactcc tcctcgacgt cggcgcgaac gtctcgtgcc gcgtggagca ctaccgcgag
     2521 ttcgcgctga tgggtcactt ctacgcccag gaggtcctcg gcatctcgag gccgaagatc
     2581 ggcctcctct ccgtcgggga ggaggaggag aaggggaccg ggacgacgcg ggaggtcttc
     2641 ggcctcctgc ggaagtcggg cctgaacttc gtggggaact gcgaggggcg cgacgtctac
     2701 ggcgggaacg tcgacgtcga cgtgatcgtc accgacggct tcaccgggaa cgtcgtgttg
     2761 aaggcgtccg agagccttgc gcggctcgtc gaggaagcgc tgaagcagga ggtcacccgg
     2821 agcctgcagg cctccatggg gttcctcctc tcgcgcgacg cgttccggtc cttcaagaag
     2881 cggctcgact acaccgagta cggcggggcg ccgctgctcg gcgtgaaggg ggccgtcctg
     2941 atcgggcacg gcagctcgaa cgcccgggcg gtccggaacg gggtcaaggc ccttcacgag
     3001 ttcgccaggc gcagaatccc cgaaaagatc cgcgcgaagg cccgcgagct ggcgcccctg
     3061 gccgcacgct gaagagagga gacgggagat gttccacgcc gcgatcgccg gcacgggccg
     3121 ggccgtaccc gcgaagctcc tgacgaacgc cgacctggag acgctcgtcg agacgaccga
     3181 cgagtggatc accacccgga ccggtatccg ggagcgccac caggccgccg agggcgaggt
     3241 cctctcggac ttctgcgtgg aggccgcgaa gcaggcgctc gaggcggcgt ggctccaggc
     3301 gaaggacctc gacgcgatcc tgatcgcgac ggtgaccccc gaccagccga tcccgtccat
     3361 ggcctgtctc gtccagcaga agctcggggc gaagaaggcc gcggcatggg accagaacgc
     3421 cggctgctcc ggctggctct acggcctcca catcgccgac ggcctgatcc aggcgggaaa
     3481 ggccaggaac gtcctgctcg tcggcgccga gttcctgacg cgctacgtcg actacacgga
     3541 ccgggcgacg tgtgtcctct tcggcgacgg ggcgggcgcg acgatcctgc gcgccacgaa
     3601 gtcggaccgg ggcgtcctcg cctcgacgat ccggagcgac ggcgagggag cctgcttcat
     3661 ccagatgccg gggggtggct cctcgttccc gccgacccgc ccggagacgc tcgccgagcg
     3721 tctcccgttc atcaagatga tgggcgggga gacgttcaag ctcgcggtcc gctcgatggt
     3781 ggaggtctgc aaggacgtcc tcaacgagag cggattcgag cccggagagg tttcctggct
     3841 ggtcccgcac caggccaacg accggatcat ccaggccgtc ggcgagcggc tcgggatccc
     3901 gcgggagcgc tgcatcgtga acatcgagcg ctacgggaac acgagcgcgg cgtccatccc
     3961 gatcgccctc gacgaagcgg cgcgggacgg gcgcgtcaag cgcggcgacc tgatcctgtt
     4021 cacggcattc ggcgccggcc tcacgtgggg cgcggcgctc gtgaggtggt aggaatgggc
     4081 tcgctctcct tcctcttccc cgggcagggc tcgcaggtcg ccggcatggg ccgcgacctc
     4141 gtgaacgcgt tccccgaggc gcgcgccgtc tacgagcggg ccgacgccgc cctcgcgccg
     4201 ttcggcctcg cgatctcgcg cgtctcgttc gaggggaccg acgaggagct gaagcggacc
     4261 gcggtgacgc agccggcgat cctcgtccac tccgtcgccg tcttcgaggt cctgaaggcg
     4321 cggggcctga cgccgacgat gctcgcgggc cattccctcg gggagtggtc ggcgctcgtg
     4381 gcggcggggg cgctctccct cgaggaggcg gtcgtcctcg tccaccggcg cgggacgttc
     4441 atgcaggagg cggtcccggc gggggagggg gcgatgggcg ccgtgctcgg actcgacggc
     4501 gccctcgtcg ccgaggcctg cgccgaggtc gagtcggcga cgggggaggc ggtctcggcg
     4561 gcgaacttca actcgcccga gcagacggtc atcgccggga cgacgaacgc cgtcgcgaag
     4621 gccggcgagc tcctccgcca gaagggggcg aagcgggtcc tcccgctccc ggtctcggcc
     4681 ccgttccact gcgcgctgat gaagccggcc gaggagaagc tcgggccgtt cctcgacgcg
     4741 gcgccgttct ccgacctcgc cgttccggtc gtcacgaacg tcgacgtcct cgcgaacctc
     4801 tccggcgccg aggcgcgcga ggcgctccgc cggcaggtct gctcgccggt ccgctgggtc
     4861 gagacggtgc agcgcctctc gcgcgacacg gagggggcga tcgaggtcgg ccccggcacg
     4921 gtcctcgcgg gcctcgcgaa gcggatcgcg aaagactggc tcgtccggac gacgtcggac
     4981 gccgagggga tcgggaagct cctcgccgga tgaccacgaa ggcgacgacg aacgccaccc
     5041 gcgtcctcga cgcgcggaag gccgtctacg cgctccggac gttcgccgcg gcggacttca
     5101 ccgccgagga ggccgcggag aagctcggcg tccctcccgg cgaggtctgg aagacgctgg
     5161 tggcgaaggg ggaccggacc ggcctcctgg ccgcggtgat ccccaccggg acgcgtctct
     5221 cgctgaagaa gctcgctcgc gtctcgggca accggacggt cgcgatggtc tcgcccgagg
     5281 agatggagaa ggcgacgggc tacgtgaagg ggggctgctc gccgttcggg atgcggaagg
     5341 cgatgccgct ctacgtccac gaggcggcgc cggcgatgcc gcgcctcttc gtctcggcgg
     5401 gccagcgggg cgtccagctc gagatggcgg gcgaggcgtt cctcgccgcc gccggggcga
     5461 ccgccgccga cctgatcgag gaataggggg actcatgccg cacgtgatca cgctcgaggg
     5521 gaaagtcgcg ctcgtcaccg gggcgtcgca ggggatcggc gaggcggtcg cccgacggct
     5581 cgccgccgcc ggggcgacgg tcctcgtcgc cgcccgcagc gaggagaagg ccgccgcggt
     5641 cgccgcgtcg atcgcggagg cgggcggagc cgccgagagc gtccgcctcg acgtctccga
     5701 tccggcctcc gtcaccgcag ccttcaaggg gatcgtcgag cggcacggga agctcgacgt
     5761 cctcgtcaac aacgccggga tcaccgacga cggcctcctc ctgcggatgt cgaaggagag
     5821 ctgggaccgg gtgatcgcca ccgacctgac cggcgtcttc ctcgtctcgc aggaggccgt
     5881 gaagctgatg atgaagaagc gggtcgcggg gcggatcatc accgtcggct ccgtcgtcgg
     5941 cctgatgggg aacccgggcc aggcgaacta cgcggccgcg aaggcgggcg tcgtcggctt
     6001 cacgatgtcg ctcgcccgcg agatcgggtc gcgggggatc accgtgaacg ccgtcgcgcc
     6061 gggctacgtc gagacggcca tgacaggtgc gctttccgag gatcagagga aggcgctcgc
     6121 ggagaagatc gtcctcggac gcctcgggac gggggaggac gtggcggggg ccgtcctcta
     6181 cctcgcctcc gacctcgcct cgtacgtcac cggaacggtc ctgaacgtca gcggcggcct
     6241 cctcatcccc tgacgggaga ggagcttggc tgcgcttgaa ttcggcggga aagccggtaa
     6301 gaatccagcc gtttccgcca caagatggag gagttgaatg tcgaactcga ttgaggacaa
     6361 ggtcaagagc atcatcgtcg agcagctcgg ggtccaggcc gaagaggtca aggccgaagc
     6421 gagcttcgtc gacgacctcg gcgccgattc gctcgacacc gtggagctcg tcatggcctt
     6481 cgaggaagcc ttcggcatcg agatcccgga cgaggacgcc gaaaagatcc agaaggtcaa
     6541 ggacgccgtc acctacatcg agtcccacgc caagcccgcc tgagccccgg tgacggcccg
     6601 gatggaccac ctcgacgccc ggggacgccg ccgggtcgtc gtcacgggga tcggcctcgt
     6661 ctcgcccgtc ggtctcacgc gggaggagaa ctgggacgcc ctcctcgcgg ggcgttccgg
     6721 aatcggcgcc atcacccgct tcgacgcggc ggactacgcc tgccggatcg cgggggaggt
     6781 gaagggattc gacccggccc tcttcctcga ccggaaggag atcaagaagt tcgacacgtt
     6841 cgtccactac gcggtcgccg ccgcgaagga ggcgctcgcg gagtcggggc tcgcgatcac
     6901 ggaggagaac gccgaggccg tcggcgtctg cgtcggctcg gggatcgggg gcctgccgct
     6961 catcgaggag acccacaagg tcctcctcga gaaggggccg cggaggatca gcccgttctt
     7021 catcccgggg ctgatcgcga acatgtcctc ggggctgatc tcgatcctga cgggcgcgaa
     7081 ggggccgaac acggcgacgg tgaccgcctg cgccacgtcc gcccacgcca tcggcgacgc
     7141 cgtgaacatc atccgccgcg gcgacgccga cgcgatgatc tcgggaggga ccgaggcggt
     7201 cgtctcgccg ctcgccgtcg gcggcttcgc cgcaatgcgg gcgctctcga cgcggaacga
     7261 cgacccggcc gcggcctccc gtccctggga ccgcgaccgc gacggcttcg tcctcggcga
     7321 gggcgccggc atcctcgtcc tcgaggagct ggggcacgcg gtccggcgcg gcgccaccat
     7381 ctacgccgag gtggccgggt acggcatgtc gggcgacgcc caccacatct cggccccctc
     7441 cgacgacggc gacggtccgt tccgcgtcat gcgcaacgcg ctgaaggacg ccggcatgga
     7501 gccgtccgag gtcggctacg tcaacgcgca cgggacctcg acgccgcacg gggacaggat
     7561 cgagacgatc gccctgaagc ggctcttcgg ggagggggct cgatccgtgc ccgtctcctc
     7621 gacgaagtcg atgacgggtc acctcctcgg cgccgccggc ggctacgagg cgggcgtcct
     7681 cgcgctggcg atccggcgtg gggtcctccc gccgacggtc aactacacga cgcccgaccc
     7741 ggagtgcgac ctcgactaca ccccgaacgt cgcccgggag gtccgggtgc gcgcaggcct
     7801 ctcgaactcc ttcggcttcg gggggacgaa cgcctgcctg gtgatgaggg ccgtctgacg
     7861 ccccgccgcg gcggcgccgg cgtgcgggac gggcgatcgc tcgtcgatcc cgtccgcgat
     7921 tccctctatg ctcggggagc cggacgcgcc cgatgaaagc cgaccaggtt cgccggaagt
     7981 tcttcgcggg agtcccccgc tacgagtgga tcgagacgct cgggcagggg ggcgtcggga
     8041 tcgtctacaa ggcgaaggac ctcgagctcg aagaggtcgt ggcgatcaag gtcctctcgc
     8101 ccgacatcgc caaggaggac gaggtcgtcc tcgcgcgctt caagcgtgag atcaacctga
     8161 accggaagat caagcacccg aacgtcgccc gtatgtacga cttcgggatc agcggcgagt
     8221 acccctacat cacgatggag gtcgtcccgg ggaaggacct ctggacgatg atccgcgacg
     8281 agggccggct cccgcccgcg cgggcggtgc cgatcctccg ccagatcgcg cgcggctgct
     8341 cggcgatcca cgagctgggc atcgtccacc gcgacctgaa gagccagaac gtcgtcgtcg
     8401 acgagcaggg cgccgtcgcc atcgtcgact tcggcctcgc gcgctggaac ctcggcggcg
     8461 gcttcacgct cgactcgatc atcctcggga cgccgcagta catgtcgccg gagcaggcgt
     8521 ccggcaagac ggtcgactcc cggaccgaca tctactccat cggcatcatc gcgttcgagg
     8581 ccctcacggg gcagctgccg ttcacgggcg acagcccgat cgcggtcgcg atgatgcaga
     8641 tcaacgaccc cgtccccgac ctcctcccga agttcgccga cgtctcgccg gacctgcgcg
     8701 cgatcgtcct gaaagcgctg cggaaggacc ccgcgaagag gtacgcgacg gcggccgacc
     8761 tcgaggccga cctcgcgaac gccgaccggc tgccgacgcc ccgcgagggg acggtgccgc
     8821 accccggggg cgaggacccg ctcatggcgc agctggagtc ggtactcgga gcggtggtcc
     8881 tcccggcccc gccggagaag ccgcgccggc cctctccttc ggacgcctcg gcgccggtga
     8941 cgcgcccctc cgtctctccc cgtcccgcgg cggcgcggcc ggctccgacg cctccggccg
     9001 cgccggaggc cccgtcgcgg cctccgaccg tcctgatcgt cgcctccgcg cccgccgagc
     9061 gggcggcctg gaaggcgggc ctcggcatcg gcccctgccg cgtcctggag gcggggagcg
     9121 ggccggaagc tctcgaggcg ctcgttcgcg agccggccga tctcgtcctg atggacgtcg
     9181 ccctgccggg gatggacgga ttcgacgtga cgcgggtcat caagtcgcag gcgaacctcg
     9241 tggccctccc gatcctcctc gcctccgacc ggcttgagcg aagccactac gcgttcggca
     9301 tccagtcggg cgcgaacgag ctcctcgtca agccgatcga gcccgagcgc ctgcgggagg
     9361 ccgtccgcgg cctcctccgc catcgcgggt tcccggtcga ggacggtccg gcgggggcgg
     9421 gcccggttgc cggaaacggc tgatcgcggc cgtttccgct ttacaagggc ggggcagagt
     9481 cccgatcatc ggccgatgtc gcgcgactcc gggccggacg atcccgtcgg agaggcggag
     9541 gctcacgcgg agcccggccg gccggcggag aagcgggcag gctactggga ccccacggcg
     9601 tccgggcgtc accagatcag ccgcccgatc gccatcgacg gcctccgcgc cgaccgggcc
     9661 cgcatcgagc tcgcgccccg cgtcgtccgg cgcgggggca cctactacga cgactcgtac
     9721 cacgtcgtcg acgcggcggc gcgccagctc gcgtcgatct tcacgctgga gcagctcgtc
     9781 atggcgacgg tcgctctccg cgtccacatg cgacgcgggc gggagcacct cggccggaag
     9841 cgggagctgc aggagcggtg gacggtcctc gcccaccggc tcatgaagct gcgcgcggag
     9901 gagaggcacc tcgcgctgcg cgagcagcgt atcgccgagg agcacgccag cctcgcgggc
     9961 gcggagtcgg agcggccggg caaggccgag ctcctcacgc tcgaggaggc cgaggtcgcg
    10021 caggcccggc aggcctggag ggacgagagc cggcgcgcct gggaggcgtg gagccgcctc
    10081 gagtcggagg agggggccct cctggccgga gcgcccggcc tcgagcagac cgaggacctc
    10141 aagcagccgt acatcctcct cctcgtcggg gcgcggaggg cggcgccgcg aagcgtggcg
    10201 gccgcgatgc acaccctcgt cgacgccctg cagcggcccc tgtcgcggac cgaccagatg
    10261 ctcctcgaga tcgcgttccg ccggtgcatc cgcgcgggct cgccggagga cgagcgggac
    10321 ctcgagcggt gggagcacga gctcctcgcc gccgccgagc gcgacttcgc cgcggagttc
    10381 ccgtgggcgc ggccctacct cgggaacggc tgacggggcg gcggggccgc cgcggctccc
    10441 gccgcgcgaa gccctgcccg gacggcgtcc cgtgtgcctg atcgtcctcg ccctccacgc
    10501 gcaccctctc tacccgctcg tcgtcgccgc caaccgcgac gaggcgctcg cgcggccctc
    10561 ggctccggcg gacttctggc cggacgcgga cggcctcctc gcgggacggg acgaggagtc
    10621 gggcgggacg tggatgggcg tcacgcgctc cgggcgcttc gccgcgctga cgaacgtccg
    10681 cgacggacga ccgcccgaac cgctcgcccc gtcgcgcggc gggctcgtcg tccgtttcct
    10741 cgcgggcggg gacgagcctc tcgcgcacgc ccgggcggtc gccgccgagc gcgtccggag
    10801 gaacggcttc aatctcctcg ccggcgcggg cggccggctc gcctgggtct cgaacgccgg
    10861 cggcggaccg gcagaggtga cgccgggcgt ccacgcggtc tcgaacgcgg ccctcgactc
    10921 gccctggccg aagtccctcc ggacggccgc cggcctgcgt cgcgtcctcg aggacggagg
    10981 cgcgatcgac cccgaggagc tcttcgcgat cctgtcggac cgtcggatcg ccgccgacga
    11041 cgagctgccg gagaccgggg tcccgctcga ggtcgagcgg ctcctgtcgg cgccgttcat
    11101 cgtcgcgccc ggctacggca cgcgctcctc gacgctcctc ctcgcggccc gggacggccg
    11161 ggccgagctc ctcgaacgac gcttcgacac gtcgtttcgc gaatcgggga cgagccgttt
    11221 cgagctcgac ttcccgggct ggaacggcag agtcgagacg cggtttccgt cgtcctgaga
    11281 tcgaccttca ggaccgctga atctg
//
LOCUS       DAOONL010000105        11233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6517_length_11233_cov_20.378154, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000105 DAOONL010000000
VERSION     DAOONL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_11233_cov_20.378154"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLP50_13210"
     CDS             complement(<1..744)
                     /locus_tag="PLP50_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPA52550.1"
                     /translation="MKPAAVLTDLDGTLLEHGGVLGAEARAAIARLRGLGIPVVPLTS
                     KTEVELRTLLAELDSGGVGSFENGAGIVSPSGVALSEKAIPLARLAGHLEELARSAKL
                     VLTSALALSVAEIRRITGLPEAKVPAMLARGFGLPFLAPEGAGPALEAAASGLPGVRL
                     TRGGQFWHLSGDHGKEDALDLLVATGAAARPFAGFGDAPNDAGFLARCDVAVLLPRAA
                     GGVDPALAAAVPGARVAPFPTGRGWAAALD"
     gene            complement(797..2416)
                     /locus_tag="PLP50_13215"
     CDS             complement(797..2416)
                     /locus_tag="PLP50_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPA52551.1"
                     /translation="MTDGTANADAWDFVIVGSGFGGSVSALRLSEKGYRVLVLEKGPR
                     RGANDFAKTTWDLRRWLWMPGLGCRGIFRMTFLPHVTALSGVGVGGGSLVYANTLPVP
                     KRPFFEAPSWSRLARWEEELAPHYRTALAMLGAARTPFLTKPDEVIRDIGRQIGREEG
                     FESTNVAVFFGREGKGDKPGDVVPDPYFGGKGPERTVCTRCGGCMVGCRHGAKNTLEK
                     NYLFLAEGLGAVVKSDTEVTWVKPLPGGGYELTARVGGAPVPALRGVERFTARNVVFS
                     GGVLGTVKLLLELKESPEGLPALSPKVGDFVRTNSEVLVGVVTTKDEDLSQGVAIGSI
                     LHTDEHSHLEPVRYSKGSGFLRLLALPFAPGDTLFQRFAHAISTILKSPWKATKSFWV
                     PDWAKRTMILLYMRTVDGHLKMTLARSWRTGFRRGLSSVAGGGAGAKAWHPEANDLTR
                     RACEALDGFPQSILTETLLGIPTTAHILGGCPMGDAPESGVIDHRHRVFGYDGLWVVD
                     GSAISANPGVNPSLTITALAERAMTFVPPKA"
     gene            complement(2472..2771)
                     /locus_tag="PLP50_13220"
     CDS             complement(2472..2771)
                     /locus_tag="PLP50_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HPA52552.1"
                     /translation="MPQPAARVSDMHTCPMQSPAVPPVPHVGGPILPPGCPTVLIGGL
                     PAARVTEMATCVGPPDTIVMGSVRVLIGGLPAARMGDPTVHGGVIVTGCFTVLFD"
     gene            complement(2775..3374)
                     /locus_tag="PLP50_13225"
     CDS             complement(2775..3374)
                     /locus_tag="PLP50_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52553.1"
                     /translation="MSSSASPRSAATILPRLDASAEAKALLTPDAQPSDYVALLVEKE
                     LFTDAAGFVAQWLEPYDAVAWACEAARAALGEAPPAERAALEAAERWLATRDEKDRRA
                     AAEAAERLGHRSAPAMAAAAAGWTGGSLSSADLPAVAPPPGLAGKGASAAVLLAASSA
                     APEVPERLRGLIWRGVDLAEGRRKTGRGPADQARTGREG"
     gene            complement(3385..5757)
                     /gene="tssI"
                     /locus_tag="PLP50_13230"
     CDS             complement(3385..5757)
                     /gene="tssI"
                     /locus_tag="PLP50_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HPA52554.1"
                     /translation="MSDYTQSNNTFRITTPLGPDALLLRRIRGSEGISELFDLDLEVL
                     APNETEVSFDALLGQSVTVAVHGVDGSPVRHFNGICAELEAGPREALEADKKTFTSYR
                     LRVVPKAWLLGLVHRSRVFQEIAVPDILKAVFKGIDSSFRLERTYDRREMCLQYRESD
                     LAFACRLMEEEGIRYYFTHSAESHLLVVTDRGGHEDLPGPSPLRYDPIEGGARDEDRI
                     TSWHKRQALTRGKVTLRDRSFEVPTSDLEKQIVLKDALKAGTVTHKLRLAEADERELY
                     AFPGGHAKPFDGVAPGGGDRTGVLGDIFPYGETRARVIMEREETRAIEIEGAGNVRHL
                     VAGFQVTLDRHHDGNGAYLVTRVRHEGQNGSFRSDEVETRYENTFTCLPAEAVYRPPR
                     LTHRPVAQGPETAIVVGSPGEEIYVDKYGRIKVQFYWDREGKKDVTSCAWLRVVTPLA
                     GAQWGSYAFPRIGQEVLVAFQHGDPDEPVVLGALYNAENMPPYPLPANRVRTGLKTRS
                     TLKGAADEFHEVRLDDTKDKEEIFVQSQRDFHRLVKNNDLLQVGVKKGDKEPPDGSQK
                     QEIWKNRTVNVSKGDDCLNVGRLPGETQPAVEARSGANSGQSPSCGGNLTTWVANDRT
                     TTLNAGNDTLVVGVQAMGKSTPSLPVHQGNQVLEVQNDRTATVNYGNDRLTVRKGNLD
                     IKLEGTMPYGKVTIEAPNAIELKVGMNHVKIDTMGIELKFGTNQLKLDNLAAELKSTA
                     GSLKLDAASATLRGIAATLQGDATTDVKAPIVNVRADGIASIGGSLTKIG"
     gene            complement(5815..8148)
                     /locus_tag="PLP50_13235"
     CDS             complement(5815..8148)
                     /locus_tag="PLP50_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPA52555.1"
                     /translation="MVLDPPQALLPPLTTIPFGRGLDGRPIRHVNGKLVVGTIRHAID
                     LTLERELDALPAAPTPTERNAREAVVRARLLVRLVERLNGLISDARYHVTSETLLVDG
                     NNYSREFELYVNELCREFAGVDYFYFTRGGKVVPPTIAAIFRPFGIRQAYALLSRAAE
                     KFVETDVRVVETTARSAVVRWYAASQLDDVREELQERWLLASCAAYQGTCAAIPALLY
                     PGQAWAEVDELKCQARGDDCCEWRFTWEDPHPHATRLLLAGAGVSTGLLGWALSGAPG
                     SAVAAPLVLLPLLGAWHVARVRRLQHDRQEQDRLIAAQRATAESQVDRVQAAYADVQQ
                     STLALRGKVAELSALREVALAVSASLDPFEVVDRSLEAVRRHLDYDRAVVLLVDETRS
                     VLSCARSHGLSPDQASLLSRVSIRLDDPLSAIAPAIRDAVAFRLRDLPGPPGAATRQL
                     VPILGSEEVFVAPLLSRGRSVGVLIVDNGTTRRPLPGEGLELLTAVASTIGVAVENAR
                     LYSQIEQQRRTLEARVRERTADLERATEEAEAANRAKSAFLANMSHELRTPLNAIIGY
                     SEMLLDERAGGDADGAASDLGRIRAAGLYLLGLINDVLDLSKVEAGKTELNLEPFTLS
                     ALLRDVSNDVRALVERGGNRFAVDVASEETLYGDPRKVRQVLLNLLGNAAKFTEGGAV
                     ELRARRDGEEVLLSVADTGIGMTPDQVGRLFTAFWQGDPASPRNYGGTGLGLAISRHY
                     ARLMGGDVSVVSEAGRGSTFTLRLPVRVAGASPGRSA"
     gene            complement(8204..9073)
                     /locus_tag="PLP50_13240"
     CDS             complement(8204..9073)
                     /locus_tag="PLP50_13240"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPA52556.1"
                     /translation="MERESENVFRGWRRPGPRPPGGLEPGEGETLDYLCGSFRVFQAA
                     RGHRYSTDDLLVAWWGVRSAPRVERLLDLGSGIGSIAMAAAWKLPAARVVTVEAQPFS
                     IALARKSVRYNGLADRFRILEGDLRDPAVLEGEAPFDLVLGAPPYFERGDATEACHPQ
                     AVPARITLRGGVGAYAEAARRSLAPGGRFAFVWPVARDGEARDALERERLLLLARRDV
                     VFREGEPPLVALFDAARVEDLPASLATAAGFPRDEPPLVVRRADGRHTPEYAAVRLSM
                     GFPPGDLGPRRGG"
     gene            9275..9925
                     /locus_tag="PLP50_13245"
     CDS             9275..9925
                     /locus_tag="PLP50_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPA52557.1"
                     /translation="MRSERDRYLDAILAGRRQDAVEAALGALERGMDVRDVYVDVLQE
                     SLYEVGRLWQAGRISVAAEHLATAITQLVVARLYEKLPRSEATRGRVVLTGVEGELHQ
                     VGGNMIADALEADGWDVRFLGTDVPGPDVLDAIAAHRADLFGISCTLRQNVPKVASLV
                     DEVRSRFGESRPRILVGGGAFRTAGGSPLDVGADAWAPDMREAIAVARTLGADGGA"
     gene            9931..11220
                     /locus_tag="PLP50_13250"
     CDS             9931..11220
                     /locus_tag="PLP50_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52558.1"
                     /translation="MEALPLPLSDRLREAVRPIHERIEKLPFFTSLSARALPFERYLD
                     QLRAMAIVEATLERVLLSTRHPAVAAARDAVPSRLEALLSDLASFDRRGPLPDDPAPA
                     ATALGLSRALLLLSADRPAALLGPLYVTTGMTLGNLVHLQDARACAGGGGTGWYEGHG
                     AGTAPAFRRLCAVLDGLTLAPGEAAAAIDAAVACAEGLERVHAVLDPATRPLRRFLAT
                     TLNPEAGSHPVPSDPSELSAALRAGARCLEEYPYFLSRYGERGRRFTGSDAAWLVSLT
                     GLPSADVSGQVGWLASVLARKGMPSLLLERQLVLLAEELRAGGVPRDGGVLESAARSL
                     ADRRREALPEEVAARLAGTFVASSGHGPEEERRAAADLLLAAVADEAAGLAGTVRAVT
                     EWFRDPRYPGSWNEAVDVLVRDALAAAARRGPEGPAG"
BASE COUNT         1442 a         4139 c         4077 g         1575 t
ORIGIN      
        1 gtcgagggcg gcggcccagc cgcggcccgt cgggaagggg gcgacgcgcg cgcccggcac
       61 ggccgccgcg agcgcgggat cgacgccgcc cgccgcgcgc ggcaggagga cggcgacgtc
      121 gcaacgcgcg aggaagccgg cgtcgttcgg cgcgtcgccg aagcccgcga aggggcgcgc
      181 cgcggcgccg gtcgccacga ggaggtcgag tgcgtcttcc ttgccgtgat cgccggagag
      241 gtgccagaac tgcccgcccc gcgtgaggcg aacgccggga aggccgctcg ccgcggcctc
      301 gagcgccggc cccgccccct ccggcgcgag gaacgggagc ccgaacccgc gcgcgagcat
      361 cgcggggacc ttcgcttccg ggaggccggt gatcctccgg atctcggcga ccgagagcgc
      421 gagcgcggac gtgagcacga gcttcgcgct ccgggccagc tcctcgaggt ggcccgccag
      481 ccgggcgagc gggatcgcct tctccgagag cgcgacgccc gagggggaga cgatccccgc
      541 cccattctcg aagctcccga cgccgcccga gtcgagctcg gcgaggagcg tccgaagctc
      601 gacctctgtc ttgctcgtga ggggcacgac gggaatcccc agcccgcgca ggcgcgcgat
      661 cgcggcgcgc gcctccgctc cgaggactcc cccgtgctcg aggagcgtcc cgtcgaggtc
      721 ggtgaggacg gcggcgggct tcatccggcc ctgtataccc cggcacgccg cgcggcgcga
      781 ccgaaaccgg tcggccctac gctttcggcg gcacgaacgt catcgcgcgc tcggcgagcg
      841 cggtgatcgt gagggagggg ttcacgccgg gattcgcgga gatcgcggag ccgtcgacga
      901 cccagaggcc gtcgtagccg aagacccggt ggcggtggtc gatgacgccg ctctcgggcg
      961 cgtcccccat cgggcagccc ccgaggatgt gcgccgtcgt cgggatgccg aggagcgtct
     1021 cggtgaggat gctctgcggg aagccgtcga gcgcctcgca ggcgcgccgc gtcaggtcgt
     1081 tcgcctccgg gtgccacgcc ttcgcccccg cgccgccgcc ggcgacggag gagaggccgc
     1141 ggcggaagcc ggtccgccac gaccgggcga gcgtcatctt caggtgcccg tcgaccgtcc
     1201 gcatgtagag gaggatcatc gtccgcttcg cccagtcggg gacccagaac gacttcgtcg
     1261 ccttccaggg actcttcagg atcgtcgaga tcgcgtgcgc gaagcgctgg aagagcgtgt
     1321 cgcccggcgc gaacgggagc gcgaggagac gcaggaaccc cgagcccttc gagtagcgga
     1381 cgggctcgag gtgcgagtgc tcgtcggtgt ggaggatcga gccgatcgcg accccctgcg
     1441 agaggtcctc gtccttcgtc gtcacgacgc cgacgaggac ctccgagttc gtcctcacga
     1501 agtcgcccac cttcggcgag agagccggga ggccttcggg gctctccttc agctcgagga
     1561 ggagcttgac cgtcccgagg acgccgcccg agaagacgac gttcctcgcc gtgaagcgct
     1621 cgacgccccg gagggccggg accggcgcgc cgccgacgcg cgccgtcagc tcgtagccgc
     1681 cgcccgggag cggcttcacc cacgtcactt cggtatcgct cttcacgacg gcgccgagcc
     1741 cctcggcgag gaagaggtag ttcttctcga gcgtgttctt ggccccgtgg cggcagccga
     1801 ccatgcagcc gccgcagcgc gtgcagacgg tccgctccgg ccccttcccg ccgaagtacg
     1861 ggtcggggac gacgtcgccc ggcttgtccc ccttcccttc tcgtccgaag aagacggcga
     1921 cgttcgtcga ctcgaacccc tcctctcggc cgatctgccg tccgatgtcg cggatcacct
     1981 cgtcgggctt cgtcaggaac ggcgtccgcg ccgccccgag catcgcgagc gccgtccggt
     2041 agtggggcgc gagctcctcc tcccaccgcg cgaggcgcga ccaggagggc gcctcgaaga
     2101 aggggcgctt cgggaccggg agcgtgttcg cgtagacgag cgagccgccg ccgacgccga
     2161 cccccgagag cgccgtgacg tgcgggagga acgtcatccg gaagatgccg cggcagccga
     2221 ggcccggcat ccagagccag cggcggaggt cccacgtcgt cttcgcgaag tcgttcgcgc
     2281 cgcggcgcgg ccccttctcg aggacgagga cccggtagcc cttctccgag aggcggagcg
     2341 cgctgacgct cccgccgaag cccgagccga cgatcacgaa gtcccacgcg tccgcgttcg
     2401 ccgtcccgtc ggtcatctcg cctctccttc cgaagcgtcc agtctacgcg gggctcggcg
     2461 gcacctgggc gctagtcgaa gaggaccgtg aagcagcccg tgacgatcac gccgccgtgg
     2521 acggtggggt cgcccatccg cgcggccggg aggccgccga tgaggacccg aaccgagccc
     2581 atgacgatgg tgtcgggcgg accgacgcag gtcgccatct ccgtcacccg cgccgccggg
     2641 agccccccga tcaggaccgt cggacatccg gggggcagga tcgggcctcc gacgtgcggg
     2701 acgggcggga cggccggcga ctgcatcggg caggtgtgca tgtcgctgac gcgggcggcg
     2761 ggctgcggca tcgctcagcc ctcccggccg gtccgagcct ggtccgcggg accgcggccg
     2821 gtcttgcggc gcccctcggc gaggtcgacg ccgcgccaaa tcagtccacg gagccgctcg
     2881 ggcacctcgg gcgccgcgga ggacgccgcg aggaggaccg ccgcggaggc tcccttcccg
     2941 gcgaggcccg gcggcggcgc cacggccggc aggtcggcgg acgagaggct tccccccgtc
     3001 cagcccgcgg cggcggccgc catcgcgggc gccgaccggt ggccgagcct ctcggccgcc
     3061 tccgcggcgg cgcggcggtc cttctcgtcg cgcgtggcga gccaccgctc ggccgcctcg
     3121 agcgccgcac gctcggcggg cggcgcctcg ccgagagccg cgcgcgccgc ttcacaggcc
     3181 caggcgaccg cgtcgtacgg ctcgagccac tgcgcgacga atccggcggc gtcggtgaag
     3241 agctccttct ccacgaggag ggcgacgtag tccgacggct gggcgtcggg cgtgaggagg
     3301 gccttcgcct ccgcggaggc gtcgagcctc gggaggatcg tggcggcgga ccgcgggctc
     3361 gcgctgctgc tcatcgcgtc ctcctcatcc gatcttcgtc agcgagccgc cgatgctcgc
     3421 gatgccgtcg gcccggacgt tgacgatcgg cgccttcacg tccgtcgtcg cgtctccctg
     3481 gagcgtcgcg gcgattcccc tgagcgtcgc gctcgcggcg tcgagcttca gcgagccggc
     3541 cgtcgacttc agctcggcgg cgaggttgtc gagcttcagc tggttcgtcc cgaacttcag
     3601 ctcgatcccc atcgtgtcga tcttcacgtg gttcattccc accttcagct cgatggcgtt
     3661 cggcgcctcg atcgtcacct tgccgtaggg catcgtccct tcgagcttga tgtcgaggtt
     3721 ccccttccgg accgtcagcc ggtcgttccc gtagttgacc gtcgcggtcc ggtcgttctg
     3781 cacctcgagg acctggttgc cctgatgcac gggcagcgag ggcgtcgact tgcccatcgc
     3841 ctggacgccg acgacgagag tgtcgttgcc cgcgttcaga gtcgtcgtcc ggtcgttcgc
     3901 gacccacgtc gtcaggttcc cgccgcacga cggggactgg cccgaattgg cccccgaccg
     3961 cgcctcgacg gccggctgcg tctcgccggg aagccgcccg acgttcaggc agtcgtcccc
     4021 cttgctcacg ttgaccgtcc ggtttttcca gatctcctgc ttctggctgc cgtccggcgg
     4081 ctccttgtcg cccttcttca ccccgacctg gaggaggtcg ttgttcttca cgagccggtg
     4141 gaagtccctc tgcgactgga cgaagatctc ttccttgtcc ttcgtgtcgt cgaggcggac
     4201 ctcgtggaac tcgtcggccg cccccttcag cgtggagcgg gtcttcagcc ccgtccggac
     4261 ccggttcgcc gggagcgggt aggggggcat gttctcggcg ttgtagagcg cgccgaggac
     4321 gacgggctcg tccgggtcgc cgtgctggaa cgcgacgagg acctcctgcc cgatccgcgg
     4381 gaacgcgtag ctcccccact gcgcgccggc gagcggcgtc acgacccgga gccacgcgca
     4441 gctcgtcacg tccttcttcc cctcgcggtc ccagtagaac tgcaccttga tccgcccgta
     4501 cttgtcgacg tagatctcct cgcccggcga gccgacgacg atcgccgtct ccgggccctg
     4561 cgcgacgggg cggtgcgtga gccgcggcgg gcggtagacc gcctccgccg gcaggcaggt
     4621 gaacgtgttc tcgtaccgcg tctccacctc gtccgaccgg aaggagccgt tctgcccttc
     4681 gtgtcggacg cgcgtcacga ggtaggcgcc gttgccgtcg tggtgccggt cgagcgtgac
     4741 ctggaacccg gcgacgaggt ggcggacgtt gcctgccccc tcgatctcga tcgcccgtgt
     4801 ctcttccctc tccatgatga cccgggcccg cgtctcgccg taggggaaga tgtctcccag
     4861 cacgccggtg cgatccccac cgccgggcgc gacgccgtcg aacggcttcg cgtggccgcc
     4921 cggaaaggcg tagagctcgc gctcgtccgc ctcggcgagg cggagcttgt gggtcaccgt
     4981 ccccgccttc agcgcgtcct tcaggacgat ctgcttctcg aggtcgctcg tcggcacctc
     5041 gaacgagcgg tcccggagcg tgaccttccc gcgcgtgagc gcctgccgct tgtgccagga
     5101 ggtgatccgg tcctcgtcgc gggcgccccc ttcgatcggg tcgtagcgga gcgggctcgg
     5161 tcccgggagg tcctcgtgcc ccccgcggtc cgtcacgacg aggaggtggc tctcggcgga
     5221 gtgggtgaag tagtagcgga tcccctcctc ctccatcagg cggcaggcga acgcgaggtc
     5281 gctctcgcgg tactgcaggc acatctccct ccggtcgtac gtcctctcga gccggaacga
     5341 gctgtcgatc cccttgaaga cggccttcag gatgtccggg accgcgatct cctggaagac
     5401 gcggctccgg tggacgaggc cgaggagcca ggccttcggg acgacgcgga gccggtagga
     5461 cgtgaacgtc ttcttgtcgg cttcgagcgc ttcccgcggc cccgcctcga gctccgcgca
     5521 gatcccgttg aagtggcgga cggggctccc gtcgacgccg tggaccgcca cggtcacgga
     5581 ctgcccgaga agcgcgtcga acgagacctc ggtctcgttc ggagcgagga cctcgaggtc
     5641 gaggtcgaag agctcggaga tcccctcgga gccccggatg cgccgaagca ggagggcgtc
     5701 cggcccgagc ggcgtcgtga ttcggaaggt gttgttcgac tgcgtgtagt ccgacatcct
     5761 cagcgggtcc tttcgcgtcg gctggccgtc tgtccggggc gctttcgctc gagcttacgc
     5821 cgaacggccc ggagacgcgc ccgcgacccg gaccgggagg cgcagggtga acgtcgagcc
     5881 gcgtcccgcc tcgctgacga ccgagacgtc ccctcccatc aggcgggcgt aatggcggct
     5941 gatggcgagg ccgaggccgg tccccccgta gttgcgcgga gacgcggggt ccccctgcca
     6001 gaacgcggtg aagaggcggc cgacctggtc cggcgtcatc ccgatccccg tgtccgcgac
     6061 cgagaggagg acctcctccc cgtcgcggcg cgcccgcagc tcgaccgccc ccccctcggt
     6121 gaacttcgcg gcgttcccga ggaggttcag gagcacctgg cgcaccttcc gggggtcgcc
     6181 gtagagcgtc tcctcggagg cgacgtcgac ggcgaagcgg ttccctcccc gctcgacaag
     6241 cgcgcggacg tcgttcgaga cgtcgcgaag gagggcggag agtgtgaacg gctcgaggtt
     6301 cagctccgtc ttccccgcct cgaccttcga caggtcgagg acgtcgttga tgagcccgag
     6361 gaggtagagg ccggcggcgc ggatcctccc gaggtcggag gcggcgccgt ccgcgtcgcc
     6421 gccggccctc tcgtcgagga gcatctcgct gtagccgatg atcgcgttca gcggcgtccg
     6481 aagctcgtgg ctcatgttcg cgaggaacgc gctcttggcc cggttcgccg cctccgcctc
     6541 ctcggtggcg cgctcgaggt cggccgtccg ttcgcggacg cgcgcctcga gcgtccgcct
     6601 ctgctgctcg atctgcgaat agaggcgcgc gttctcgacg gcgacgccga tcgtcgaggc
     6661 gaccgcggtc aggagctcga gcccctcgcc ggggagcggc cggcgcgtcg tcccgttgtc
     6721 gacgatcaga acgccgacgc tccgaccgcg cgacaggagc ggcgcgacga agacctcctc
     6781 cgagccgagg atcgggacga gctggcgcgt cgcggcgccg ggcggccccg gaaggtcgcg
     6841 cagccggaag gcgacggcgt cccggatggc gggagcgatc gccgagagcg ggtcgtcgag
     6901 gcggatcgag acgcgcgaga ggagcgaggc ctggtccggc gagagcccgt ggctcctggc
     6961 acaggagagg acgctccggg tctcgtcgac gaggaggacg accgcccggt cgtagtcgag
     7021 gtgccgccgc accgcctcga ggctccggtc gacgacctcg aacgggtcga gggaggccga
     7081 gacggcgagg gccacctcgc gcagcgccga gagctccgcg accttcccgc gcagcgcgag
     7141 cgtcgactgc tggacgtcgg cgtaggcggc ctggacgcgg tcgacctggc tctcggcggt
     7201 cgcgcgctgg gcggcgatga gccggtcctg ctcctgccgg tcgtgctgga gccggcggac
     7261 gcgcgcgacg tgccaggcgc cgaggagcgg aaggaggacc agcggcgcgg cgacggccga
     7321 gccgggcgct ccggaaaggg cccacccgag gaggcccgtc gacacgccgg cgccggcgag
     7381 caggaggcgc gtggcgtgcg gatgggggtc ctcccaggtg aagcgccatt cgcagcagtc
     7441 gtccccgcgc gcctggcact tcagctcgtc gacctccgcc caggcctggc ccgggtagag
     7501 cagggcgggg atcgcggcgc aggtcccctg gtaggccgcg cagctggcga ggagccatcg
     7561 ctcctggagc tcttccctca cgtcgtcgag ctgcgaggcc gcgtaccacc tcacgacggc
     7621 cgagcgggcc gtcgtctcga cgacccggac gtccgtctcg acgaacttct ccgcggcgcg
     7681 cgacaggagc gcgtacgcct gccggatccc gaaggggcgg aagatcgcgg cgatcgtcgg
     7741 cgggacgacc ttccctcccc gcgtgaagta gaagtagtcg accccggcga actcgcggca
     7801 gagctcgttc acgtagagct cgaactcgcg agagtagttg ttcccgtcga cgaggagcgt
     7861 ctccgacgtg acgtggtacc tcgcgtcgga gatcaggccg ttcagcctct cgacgagccg
     7921 cacgaggagc cgcgcccgga cgaccgcctc ccgggcgttt cgctcggtcg gcgtgggagc
     7981 cgccggaagc gcgtcgagct cccgctccag cgtcaggtcg atcgcgtgcc ggatggtccc
     8041 gaccacgagc ttcccgttga cgtgccggat cggccttccg tcgaggccgc gcccgaacgg
     8101 gatcgtcgtc agcggcggca ggagcgcctg cggaggatcg aggaccacgt gtgccggaac
     8161 gatacggcat ccgccgtcgg gggactctcg aaaggcgcgc ggctcagccg ccccgtcgcg
     8221 ggccgaggtc tcccggcggg aaacccatcg agagccgcac ggcggcgtac tcgggcgtgt
     8281 ggcgcccgtc cgcgcgccgg acgacgagcg gcggctcgtc gcgcgggaag ccggccgcgg
     8341 tcgcgaggga ggccgggagg tcctcgacgc gagcggcgtc gaagagcgcg acgagcggcg
     8401 gctccccctc gcggaagacg acgtcccggc gggcgaggag gaggagtctc tcgcgttcga
     8461 gcgcgtcgcg ggcctccccg tcgcgcgcca cgggccagac gaacgcgaaa cgccccccgg
     8521 gcgcgagcga ccgccgggcc gcctccgcgt acgcgcccac gccgccccgg agcgtgatcc
     8581 gggccgggac cgcctgcggg tggcacgctt ccgtggcgtc gcccctctcg aaatacgggg
     8641 gcgcgccgag gacgaggtcg aacggagcct ctccctccag gaccgccggg tcacggaggt
     8701 ccccctcgag gatgcggaaa cgatccgcga ggccgttgta gcggacgctc ttcctcgcga
     8761 gggcgatcga gaacggctgg gcctccaccg tcacgacgcg ggccgccgga agcttccacg
     8821 ccgcggccat cgcgatcgag ccgatgccgg agccgaggtc gaggagcctt tcgacgcgcg
     8881 gcgcgctccg gacgccccac caggcgacga ggaggtcgtc ggtggagtac cggtggccgc
     8941 gcgccgcctg gaagacccgg aagctcccgc agaggtagtc gagcgtctca ccttcgcccg
     9001 gctcgagccc gcccggcggc ctcggcccgg ggcgtcgcca gccccggaag acgttctcgc
     9061 tctcccgttc catcggacgg agcgtacagg gagagcgctc cctccgcgat ccccggcgcc
     9121 ggcgttcgcg acccggccct tgggccttcg ggtgtccgat gccgtgactc cgctcgacgc
     9181 ggccggtcga tccatatcag actccccgtt acgggcctcg gtccggagag ccggcaaggc
     9241 cgtgtcgacc ggcgcggcga aggggaggag cgacatgcgt tccgagagag accggtacct
     9301 cgacgcgatc ctcgcgggac gccggcagga cgcggtcgag gcggcgctcg gcgcgctcga
     9361 gaggggcatg gacgtccgcg acgtctacgt cgacgtcctg caggagtcgc tctacgaggt
     9421 cggccggctc tggcaggccg gccgcatctc cgtcgctgcg gagcacctcg cgacggccat
     9481 cacccagctc gtcgtcgcac gtctctacga gaagctcccg cggagcgagg caacgcgcgg
     9541 acgggtcgtc ctgaccggcg tcgaggggga gctccaccag gtgggcggga acatgatcgc
     9601 cgacgcgctc gaggcggacg gctgggacgt ccgcttcctc ggcaccgacg tcccggggcc
     9661 ggacgtcctc gacgcgatcg ccgcgcatcg cgccgacctc ttcggcatct cctgcacgct
     9721 ccggcagaac gtcccgaagg tcgcctcgct cgtcgacgag gtccggtcga ggttcgggga
     9781 gtcccgcccc cgcatcctcg tcggcggcgg cgcgttccgg accgccggcg ggagcccgct
     9841 cgacgtcggc gccgacgcct gggcgcccga catgcgcgag gcgatcgccg tggcccggac
     9901 gctcggggcg gacggaggag cgtgacgccg atggaggcgc tccccctccc gctctccgac
     9961 cgccttcgcg aggccgtccg cccgatccac gagcggatcg aaaagctccc gttcttcacc
    10021 tcgctttcgg cacgagccct ccccttcgag cgatacctcg accagctccg cgcgatggcg
    10081 atcgtcgagg cgacgctcga gagggtgctc ctgtcgaccc gccatcccgc ggtcgcggcc
    10141 gcgcgggacg cggtcccctc ccgcctcgag gccctcctct cggacctcgc ttccttcgac
    10201 cgccgcggcc cgctccccga cgacccggcg ccggcggcca ccgcgctcgg gctctcccgg
    10261 gcgctcctgc tcctttcggc cgaccggccg gcggcgctcc tcggcccgct ctacgtcacg
    10321 accgggatga ctctggggaa tctcgtccac ctccaggacg cccgcgcctg cgccggtggc
    10381 ggcggcaccg gctggtacga agggcacgga gccgggaccg ctccggcgtt ccgccgcctc
    10441 tgcgccgttc tcgacgggct caccctcgct ccgggcgagg cggcggccgc gatcgacgcg
    10501 gcggtcgcct gcgccgaagg cctcgagagg gtgcacgcgg tgctcgaccc cgcgacgcga
    10561 cctctccgac gcttcctcgc gacgaccctg aaccccgagg ccgggtccca cccggtcccc
    10621 tccgatcctt ccgagctgtc cgccgcgctc cgcgccgggg cgcgctgcct cgaggagtac
    10681 ccgtacttcc tgtcccggta cggggagcgg ggccggcgct tcaccggcag cgacgcggcc
    10741 tggctcgtct cgctgaccgg cctcccgtcc gccgacgtct ccggacaggt cggctggctc
    10801 gcgagcgtcc tcgcgcggaa ggggatgccg tcgctcctcc tggagcgcca gctcgtcctg
    10861 ctcgcggagg agctccgcgc gggcggcgtc ccgcgcgacg gaggggtcct cgagagcgcc
    10921 gcgcgctccc tcgcggaccg tcgccgggaa gctctccccg aggaggtcgc cgcgcgcctc
    10981 gcggggacct tcgtcgcctc ctccgggcac ggtcccgagg aggagcggcg cgcggccgcg
    11041 gacctcctcc tcgccgccgt cgccgacgag gcggcgggtc tggccgggac ggtgcgcgcc
    11101 gtgacggagt ggtttcgcga cccgcgctac cccgggagct ggaacgaggc cgtggacgtc
    11161 ctcgtgaggg acgccctcgc cgccgccgcg cggcgcgggc cggaaggccc ggccggatga
    11221 cgccctcccc gcc
//
LOCUS       DAOONL010000106        11203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6547_length_11203_cov_13.652853, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000106 DAOONL010000000
VERSION     DAOONL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6547_length_11203_cov_13.652853"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLP50_13255"
     CDS             complement(<1..206)
                     /locus_tag="PLP50_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52559.1"
                     /translation="MKVRNRATPFQAQSRDRTRGSLRSIVVWYFREVYGRFEGPGVLP
                     FYCDPERRDRKFDLSPSARLTPEAG"
     gene            308..769
                     /locus_tag="PLP50_13260"
     CDS             308..769
                     /locus_tag="PLP50_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HPA52560.1"
                     /translation="MATSRTTKSTRPPKQKRIPLPPGFVPVTVVNVRFEECDVYVGRS
                     FGRFRSSDGFFGNPFKGDHSISKFEEYFFDRVETDPEFARRARALHGKRIGCWCVPGP
                     CHAETLAAFADLERLRELGVDPRYVEGYEELDELLSREEAKAKKGSRPAKR"
     gene            complement(749..1537)
                     /locus_tag="PLP50_13265"
     CDS             complement(749..1537)
                     /locus_tag="PLP50_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52561.1"
                     /translation="MKRDAIGFDRDLELAWLEATAARVAEGMPPPQVREFLWTLLAGK
                     VAGDAPRSGRGKTATVLLHIWSDVPAETVPLRNDCLETLTRLSGEQQLLAHWTMAMAT
                     YPFFVEVVSTVGRLLALQETVALNQVTRRIAEKWGERSTAVRACRRVVRSIVRWGALL
                     DTTKHGVYARRIAPIPVGGVEALLLAEGLLRGLGRDTIALDEVPSSPAFFPFLASVEI
                     STVGRSGRLELLREAMDRELVALRGTSAGREEGRRASRSPFRRP"
     gene            complement(1534..3594)
                     /gene="pglZ"
                     /locus_tag="PLP50_13270"
     CDS             complement(1534..3594)
                     /gene="pglZ"
                     /locus_tag="PLP50_13270"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HPA52562.1"
                     /translation="MTTPSPREAGWRHYLLKHFTPAAAAASRVTVVHDPDDLISEAGV
                     LEGLRASGFDVIPFDDPIAFRYAYETRYRESWDRGEETPLVVVLRVREGGDVAVPYDL
                     LSRAQRHGRVLRFGLSELFPQLAPSVVEALDRSEFEPLWNAVRDHGPGELGENATKDF
                     VLRHVFDLAPELVRAPAALLAMLLRRHFRGTQLPRTLDARLVEKLQGTGRFGDWPLQT
                     IVPDRNQFFRFLEERWAHFVKRKASANGEELAEAGGGDWLKVPGPVDLPFDHSDVRVW
                     IDNLFVEGILEPMTGVPTEPFAGTWMAAGVAGTKGAGLETRLEKLAVRIEAEIPGEEA
                     DPQAWIATAKLWAELLDVSWQVHPGAEVSARVEPLRNRIEESFQLWLRRWYSSLANLP
                     FWPKPTMVHHVPHWMAHTALKADGGGGRQALLVLDGLSLGQWTILRDSLRTLPPTTRL
                     TEGGAFAWVPTLTSVSRQAIFFGNAPLFFASSLLTTSRDESHWRRFWEDRGVPRADVD
                     FLLQRQQEPDGTFFGRIQTAASRPNLRRLAVVISIVDGMLHGSVTGSRGVAALLRDWG
                     STGSFRSLLTGLLERGFRIVLTSDHGNVEAEGLGRPNLGAVADERGERVLLLPDESIR
                     SQVNRDFPTAEPWPAWGLPPSPVPLLAPGRSAFLAPGARAVVHGGTSLEEVIVPFIQL
                     EVHE"
     gene            complement(3591..6308)
                     /locus_tag="PLP50_13275"
     CDS             complement(3591..6308)
                     /locus_tag="PLP50_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPA52563.1"
                     /translation="MAEERAPGWAYHAETGEALRLLERHELWGQTVYEAWSVEERRPL
                     RLAENELTWEAPAGFRSSPHFVPFAAAAGRIADALGREELVSPFSAPVLPLPHQLYAV
                     SRALSGERIRYLLADEVGLGKTIEAGLILRELKARGLVRRTLVVAPAGLVGQWREEMK
                     TRFGEEFRAIEPGTFADARQLLGLDPVENLWRLHDQVICSVDSVKPVDGRRGWTREQV
                     ARVNRERHEDLVTAGWDLVIVDEAHRLGGSSENVARYRLGEALGLAAPYLLLLSATPH
                     QGKTDAFRRLLGFLDPEAFTAGVPVNRENVAPVVIRTEKRNAIDAEGNPLFRLRQTRL
                     LSVRWDEVHGEEQALYEAVTEYVREGYDQAVRQSRNALAFLMILMQRLVTSSLRAIRT
                     SLERRLAVLELPEGQLSLFPEEIGDEWEELTGQEQLELALKARLKGLANERREVELLL
                     SAARRCESRGPDAKAEALLAELRRLEREEEDPELKVLLFTEFVPTQEMLAELLTGHGY
                     QVAVLNGSMSPEERRATEVEFRGPARVLISTDAGGEGLNFQFCHVVVNYDLPWNPMKL
                     EQRIGRVDRIGQTQIVQAVNLALEGSVEERVREVLEEKLRVILEEFGVDKLSDVLDAG
                     EAEVDFDRVYIEALRDPAAAAEKARLAIEAMEERIREVQQGAALLATAPRADASLARR
                     IAESPVPFWTERMRKAWAAGHDPSDGEIPGTPQLLNLIPRRIPGDPIAAVELPELSDK
                     VAGTWSLWKVSLGRGPETRSRYLPVFVSDEGRTLQPTARAVWDALAALEAEDGLLRPA
                     HVSGEATRGIAEEAEAAARAAAEGVYRDLLSRHEEETRRRKRNGERAFEARRTMIERV
                     GLLSVRHHRLQKLQAEREAWEREVTARSHVVPDLTPVVLVRLARRGELK"
     gene            complement(6301..8136)
                     /locus_tag="PLP50_13280"
     CDS             complement(6301..8136)
                     /locus_tag="PLP50_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPA52564.1"
                     /translation="MDREELLDRLRGYEWDDFEVKAAQGGIPEDSYRTVSAFANSSGG
                     WLVFGVAERRGSFEIVGVGDPDGMQNSFLGTCRSPEKFSRPIDVHAKLYDFEGRGVLA
                     FYVSPATRFDKPVKVKADRRWETYIRLGGGDHRCTAVEEARFLRDASHESYDSVASAR
                     TSVEDLDASALQWFRDHLARRNPEWAYPGLDPAAYLGELGLVRDEGELTNAAVLMFGK
                     DRLLARVKPGGVVDFRVHHSSLAPEAPDERWDDRELCERNLVATLRSLLERLRRLIPQ
                     PFAVDSRTGERRVDSPDYISIREALVNLLIHQDYSDRHRTARILWYQDATLFENPGDS
                     FAELRKMLDGGTSELRNPLLVRLLRQAGFAEQAGTGIPKIVRTWRGAQRIPPSIDNDP
                     GQKLFRLTLDWRPLKSQRDEAWYRKLGVEIDENGSRLLTYGREHGAFDVTKARLVTGL
                     PGREAVRLVSQLVTQQLLAADEVDGTAIYSLAPHLQEIWAATPAPRLGRSRKRGRVTE
                     GVTEGVNGGVTEGVSEGVSGGVNEGGGLGKADRTARLEAVIRAQPGLRLPQLAEAAEL
                     PEKTAERYLAQLRKAGRVVYRGAPRTGGYFPDEPRGKGPARRRDG"
     gene            complement(8141..10837)
                     /locus_tag="PLP50_13285"
     CDS             complement(8141..10837)
                     /locus_tag="PLP50_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPA52565.1"
                     /translation="MTFPSDEARLEYFLGKLREKLRDPDFRKIEGFPIGEDEDILRMS
                     DPPYYTACPNPFLEDFVRFHGRPFDPTEKYHREPFAVDTTVGKTDALYKAHGYHTKVP
                     HLAIVPSILHYTKSGDLVLDGFCGSGMTGVAAQWCGAAPADYRRELEAQWKAEGRTKP
                     DWGARRAILNDLGPAATFITAGYNLPFDVRAFEREARRILDEVEAELGWMYETTHKDG
                     RKGRINFTVWSEVFACPECSGEVVFVEQALDKKTKKVKDEFSCPGCGAKVSKDLLEQL
                     FETVPDPATRRPWKRIKFVPVIINYDVGAKTFEKKPDHGDLAILERIGGLPLPAEVPT
                     TAIPLEQMYHGTRLGPKGFKAIHHLFLARPAQTLAGLWKRARSGTDRTAERWNKFFVE
                     QAVWGMSVLARYAPTHYSQVNQYLNGVYYVGSQVVDVSPGYLLGGKITRTKKAFSAFS
                     GRGGHAVVETSTCERLGLRQDSIDYVFTDPPFGENIPYSDLNLLVESWHRVLTNTGPE
                     AIVDAPKNKGLHEYQDLMRRCFSEYYRVLKPGRWMTVVFSNSSNAVWRAIQEALGRAG
                     FVVADVRTLDKGTGSYRQVTSTAVKQDLVISAYKPTEAVTKKFSLGSSSEEAVWSLVT
                     EHLTNVPVFVSVKEEAEVLAERTPQMLHDRMVAFHVQRELSIPLGTADFLAGLRARFP
                     ERDGMFFLPTQVVEYDKKRAAVKKLKQIELSVNDESNAILWLRSILTEMPKTYQELHP
                     LYTRELQAWARHEKTVELKEILAQNFLCYDGKGLVPEKIHSYLSSNYKDCRNLEKDDP
                     RLRDRAVDRWYVPDEKKEADLERVRDRALLREFESYREAKERKLKSFRTEAIRAGFKA
                     AFQAKDYGTIIAVAAKLPESVLQEDEKLLVYYDVAVTRTDQD"
     gene            complement(10992..>11203)
                     /locus_tag="PLP50_13290"
     CDS             complement(10992..>11203)
                     /locus_tag="PLP50_13290"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HPA52566.1"
                     /translation="EPVDSHLVKALQEVFSGLQRVALAREALVAALGKGGAASTLTDL
                     KERLDSFLADACRGKDPSKVRIVLE"
BASE COUNT         1819 a         3834 c         3643 g         1907 t
ORIGIN      
        1 cccgcttcgg gcgtcagcct cgccgacggc gacaaatcaa acttcctatc cctccgttcc
       61 ggatcgcagt agaaggggag caccccaggg ccctcgaaac gcccgtacac ctcgcggaag
      121 taccagacga cgatcgaacg aagggacccg cgcgttcggt ctcggctctg agcctggaag
      181 ggtgtcgccc ggtttcggac cttcacggct gacggagtct gcttcaacgc cgcctgcagg
      241 caaagtcgtt tggaatcatc ggtcggagcc gagtaggctt ggaacgaagt cgccgaagga
      301 gggactcatg gcgacgagtc ggaccacgaa gtcgacccga ccaccgaagc agaagcgaat
      361 cccgctgcct cccggtttcg tcccggtgac cgtcgtgaat gtcaggtttg aagagtgcga
      421 cgtttacgtc ggacggtcct tcgggaggtt ccgctcgtcc gacgggtttt tcggaaaccc
      481 gttcaagggc gaccattcca tctcaaagtt cgaagagtac ttcttcgatc gagtggaaac
      541 cgacccggag tttgcacggc gtgcgcgggc actccacggc aaacgtatcg gctgttggtg
      601 cgtgccggga ccttgccatg ccgagactct cgccgccttt gccgacctag agcgtctccg
      661 ggagctcggg gtcgaccccc gatacgtcga aggctacgag gagctcgatg agcttctcag
      721 tcgcgaggag gcgaaggcga agaaggggtc acggccggcg aaacggtgac cgggaggctc
      781 tccgaccttc ttcgcgcccc gcagacgtcc cgcgtagcgc caccagctcc cggtccatcg
      841 cctcccgaag aagctcgagt cgaccgctgc ggcccacggt cgagatctcg acgctcgcca
      901 ggaacgggaa gaacgctgga gaggagggca cctcatcgag agcaatcgtg tctcggccca
      961 ggcccctgag gagaccttca gccaggagaa gggcttccac cccgcccacg ggtataggcg
     1021 cgatccttcg cgcatagacc ccatgctttg tcgtatccag gagcgccccc cagcgcacga
     1081 tcgaccggac gacacgtcga caggcgcgga cggccgtgga gcgttcgccc cacttctctg
     1141 caatgcgacg ggtgacctgg ttcagcgcca ccgtctcctg gagagcgaga aggcggccga
     1201 ctgtgctgac gacctcgacg aagaacgggt aggtcgccat cgccatcgtc cagtgcgcca
     1261 ggagttgctg ctcgccggag agccgggtga gcgtctcgag gcaatcgttt cggaggggga
     1321 cagtctccgc cgggacgtcg ctccagatgt gaaggagcac cgtcgccgtc ttcccgcgtc
     1381 cgctccttgg tgcatcaccg gcgaccttgc cggcgagcaa cgtccagagg aactcccgga
     1441 cctgaggcgg gggcatcccc tcggccacgc gggccgccgt ggcctccagc caggccagct
     1501 cgaggtcgcg atcgaaacct atggcgtccc tcttcattcg tgaacctcga gctggatgaa
     1561 tggcacgatg acctcttcga gggaggtgcc cccgtggacc accgctcggg ccccgggagc
     1621 gaggaaggcc gagcgcccgg gagccaggag cgggacggga ctgggcggta ggccccaggc
     1681 aggccaaggc tcagcggtcg ggaagtcacg attgacctgg gagcggatcg actcgtcagg
     1741 caggaggagc acccgctccc ctctctcgtc cgccaccgcg cccaggttcg gtctcccgag
     1801 gccctccgct tccacgttcc catggtccga ggtgaggacg atccggaaac ctctctcgag
     1861 gagtccggtc aggagagagc ggaaggaccc ggtcgaaccc cagtcacgga ggagtgcggc
     1921 gacgccgcga gagcccgtca cgctgccgtg aagcatcccg tcgacgatcg agatcaccac
     1981 ggccagcctt cggagattcg ggcggctcgc ggcggtctgg atccggccga agaaggttcc
     2041 gtccggttcc tgctgcctct gaaggaggaa gtcgacgtcc gcgcggggca ctccacggtc
     2101 ttcccagaaa cgccgccagt ggctctcgtc tctcgaggtt gtgagcaggc tggaggcgaa
     2161 gaagagcggc gcgttgccga agaagatcgc ctgccgggaa acggaggtca gggtcgggac
     2221 ccaggcgaag gcacccccct cggtgaggcg ggtcgtggga ggcagcgtgc gaagggaatc
     2281 gcggagaatc gtccactgcc cgagcgacag gccatccagg accaggagcg cctggcggcc
     2341 tcctcccccg tcagccttca gcgcggtgtg agccatccag tggggcacgt gatggaccat
     2401 cgtcggcttc ggccagaacg ggaggttcgc gagcgacgag taccagcgcc tcagccagag
     2461 ctggaaggat tcctcgatcc ggttccggag cggttcgact ctcgcggaca cctcggctcc
     2521 aggatggacc tgccaggaga cgtccagaag ctcagcccac agcttcgccg ttgcgatcca
     2581 ggcctgcggg tcagcctcct cgccggggat ctcagcctcg attcgcaccg caagcttctc
     2641 gagtcgcgtc tcgaggccgg cccccttcgt cccggcgacc ccggctgcca tccacgtccc
     2701 ggcgaagggc tcggtcggaa cgcccgtcat gggttcgagg attccctcca cgaagaggtt
     2761 gtctatccag acccgaacgt cggagtggtc gaacggaaga tctaccgggc cgggcacctt
     2821 cagccagtcg ccaccgcccg cctctgcgag ctcttctcca ttcgccgagg ccttccgctt
     2881 cacgaagtgc gcccagcgct cttcgaggaa gcggaagaac tggttccggt cgggcacgat
     2941 cgtctggagc ggccagtcgc cgaaccggcc cgtaccctgg agcttctcca cgagtctggc
     3001 gtcgagcgtg cgcggaagct gggtcccgcg gaagtgcctt cgcagcagca tcgccaggag
     3061 agcggcaggt gctcgaacga gctccggtgc aaggtcgaag acgtgccgca gaacgaagtc
     3121 cttcgtggcg ttctcgccca gctcgccggg cccgtggtcc cgcacggcat tccagagagg
     3181 ctcgaactcc gaacgatcga gcgcctcgac caccgaaggc gccagctgcg gaaagagctc
     3241 ggagaggccg aaccgaagca cgcggccgtg gcgctgcgcc cgggagagaa ggtcgtaggg
     3301 aacggcgacg tccccgcctt cccgaacccg aagaaccaca accagcggcg tctcctcgcc
     3361 gcggtcccag ctctcgcggt accgggtttc gtaggcatag cggaacgcga tcgggtcgtc
     3421 gaagggaatc acgtcaaagc cggacgctcg aaggccctcg aggacccccg cctccgagat
     3481 caggtcatcc gggtcgtgga cgacggtcac ccggctcgcg gcggcggcag caggcgtgaa
     3541 gtgcttcagg aggtagtggc gccatcccgc ctcacggggg ctcggggtcg tcacttcagc
     3601 tcccctctgc gggccaggcg caccagcacc accggcgtga ggtccgggac cacgtgcgac
     3661 cgcgccgtga cctcccgctc ccaggcttcg cgctcggcct ggagtttctg cagtcggtga
     3721 tgccggacgg aaaggagacc cacgcgctca atcatcgtcc gccgggcctc gaaggctcgc
     3781 tctccgttcc tctttcggcg ccgggtctct tcctcgtgac ggctcagcag gtcccggtag
     3841 accccttcgg ccgcggctcg ggctgccgcc tccgcctcct ccgcaatccc gcgggtggcc
     3901 tcgccggaaa cgtgggcggg cctgagaagg ccgtcctccg cctccagagc tgcgagcgcg
     3961 tcccacacgg cccgggccgt cggctgaagc gtccttccct cgtccgagac aaagaccgga
     4021 aggtagcgac tgcgggtttc cggtccgcgg ccgaggctga ccttccagag cgaccaggta
     4081 ccggcgacct tgtcggacag ctcgggtagc tcgacggcgg cgatggggtc gcccgggatc
     4141 cgacgaggaa tcaggttcag aagctgcgga gttccgggga tctcgccgtc ggaggggtca
     4201 tgcccggcag cccaggcttt ccgcatccgc tccgtccaga acggcaccgg gctctcggca
     4261 atccgccggg cgagcgaggc gtcggctcgc ggcgcggtcg caaggagggc cgctccctgc
     4321 tgcacctcgc gaatccgctc ctccattgcc tcgatcgcca gccgggcctt ttcggccgcc
     4381 gcagccgggt cgcgcagggc ttcgatgtag acccggtcga agtcgacctc ggcctcccct
     4441 gcgtcgagaa cgtcggagag cttgtcgacc ccgaactcct cgagaatgac ccggagcttc
     4501 tcctcgagaa cctcccgcac cctctcctcc accgaccctt cgagcgcgag attcaccgcc
     4561 tggacgatct gggtctgccc gatccggtcc acacggccga tccgctgctc gagcttcatc
     4621 gggttccagg gcaggtcgta gttcacgacg acgtggcaga actggaagtt cagcccctcg
     4681 ccgcccgcgt cggtcgagat gaggacgcgc gccggccccc ggaactcgac ctcggtagcc
     4741 cgccgctcct cgggcgacat gctgccgtta aggacggcca cctggtagcc gtgacccgtt
     4801 aggagttcgg cgagcatctc ctgcgtcggg acgaactccg tgaagaggag gaccttcagc
     4861 tcggggtcct cttcctcgcg ctcgagccta cgaagctcgg cgaggagcgc ttcggccttg
     4921 gcgtcggggc ctctggactc gcatcgccgg gccgccgaga ggagcagctc cacctctcgc
     4981 cgctcgttcg cgagaccctt cagccgggcc ttcagcgcga gttccagctg ctcctgcccg
     5041 gtcagctcct cccactcgtc cccgatctcc tccgggaaga gcgagagctg cccctcggga
     5101 agctcgagga cggcgaggcg ccgctcgaga gaggtccgga tcgctcgcag gctcgaggtg
     5161 acgagcctct gcatcaggat catcagaaag gcgagggcat tccggctctg ccgaacggcc
     5221 tggtcgtacc cctcgcggac gtactccgtt accgcctcgt aaagcgcctg ttcctcgcca
     5281 tgtacctcgt cccaacgcac cgaaaggagc cgggtctgtc gtagccggaa caggggattc
     5341 ccttcggcgt cgatggcgtt ccgcttctcc gtccggatca ctaccggagc gacgttctcc
     5401 cggttcaccg ggaccccggc cgtgaacgcc tccgggtcga ggaacccgag cagccgccgg
     5461 aaggcgtcgg tcttcccctg gtgcggggtg gcgctcagga gcagcaggta aggagccgcg
     5521 agcccaagcg cctcgccgag gcggtagcgc gcgacgttct cgctgctccc accgagccgg
     5581 tgtgcttcgt cgacgatgac gaggtcccag ccagccgtca ccaggtcctc gtgccgctcc
     5641 cggttcacgc gcgccacctg ctcgcgggtc cagccgcggc ggccgtccac cggcttcact
     5701 gagtcgaccg agcagatcac ctggtcgtgc agccgccaga ggttctcgac cgggtccagg
     5761 ccgaggagct ggcgtgcgtc ggcgaaggtc ccgggctcga tcgcccggaa ctcctcgccg
     5821 aaccgggtct tcatctcctc ccgccactgg ccgacgagac ccgccggcgc gacgacgagg
     5881 gttcgtctca ccagcccgcg ggccttcagc tcgcgcagga tcaggcccgc ctcgatcgtc
     5941 ttcccgaggc caacctcgtc ggcaaggagg tagcgaattc gttctcccga gagcgcgcga
     6001 gacacggcgt agagctggtg cggcaggggg aggacgggcg ccgaaaacgg ggagaccagc
     6061 tcttcccggc caagggcgtc ggcgatccgc cccgccgcgg ccgcgaaagg tacgaagtgt
     6121 ggggacgacc ggaaccccgc cggggcctcc cacgtcagct cgttctcggc gagccgaaga
     6181 ggccggcgct cctcgacgct ccacgcctcg tagaccgtct ggccccacag ctcgtgccgc
     6241 tccaggagcc gcagggcttc cccggtctcc gcgtggtagg cccagccggg ggcgcgttct
     6301 tcagccatcc cgccgccggg ccggcccctt gcctcgaggc tcgtccggga agtacccgcc
     6361 tgtcctcggg gcccctcgat agacgacccg gcccgccttg cggagctgag cgagatatcg
     6421 ctcggctgtc ttctccggca gctcggcggc ttcagccagc tgcggcaggc ggaggccggg
     6481 ctgcgcccga atcacggcct cgagccgggc cgttcggtct gccttcccca gcccccctcc
     6541 ctcgtttact cccccactta ctccctcgct tactccctca gttactcccc catttactcc
     6601 ctcagttact ccctcagtta ctctcccgcg cttccggctg cgcccgagcc gcggggccgg
     6661 cgtggcggcc cagatctcct ggaggtgcgg ggcgagggag tagatcgcgg tcccgtcgac
     6721 ctcgtccgcc gcgaggagct gctgcgtcac gagctgggac acgaggcgga ccgcttcccg
     6781 cccggggagc ccggtcacga ggcgggcctt cgtcacgtcg aaggcgccgt gctcccggcc
     6841 gtaggtcagg aggcgcgatc cgttctcgtc gatctcgacg ccgagctttc ggtaccaggc
     6901 ctcgtcgcgc tgcgatttca gcggcctcca gtcgagcgtc agccggaaga gcttctggcc
     6961 cgggtcgttg tcgatcgagg gagggatcct ctgggcgccc cgccaggtgc ggacgatctt
     7021 cgggattccc gttccggcct gctcggcgaa cccggcctgc cgaagcagcc ggaccaggag
     7081 cggattacga agctcgctcg tgccgccgtc gagcatcttc cggagctcgg cgaaggagtc
     7141 gcccgggttc tcgaagagcg tcgcatcctg ataccagagg atccgggccg tccgatgccg
     7201 atcggagtag tcctggtgga tgaggaggtt cacgagagcc tcgcggatgg agatgtagtc
     7261 gggcgagtcg acgcgccgtt cccctgtgcg cgagtcgacc gcgaacggct gcgggataag
     7321 ccgccggagg cgctccagga gcgagcgcag cgtcgcgacg aggttgcgct cgcacagctc
     7381 ccggtcgtcc cagcgctcgt ccggcgcctc gggggcgagg ctcgaatgat ggacccggaa
     7441 gtcgacgact ccgccgggct tcacgcgcgc cagcaaccgg tcctttccga acatcagcac
     7501 cgccgcgttc gtcagctccc cttcgtctcg cacgagcccg agctcgccga ggtacgccgc
     7561 cgggtcgagc cccgggtacg cccactcggg gttgcggcgc gccaggtggt cccggaacca
     7621 ctggagcgcg gaagcgtcga ggtcctcgac cgaggtccgc gcggacgcca ccgagtcgta
     7681 ggactcgtgg gacgcatccc ggaggaagcg cgcttcctcg acggccgtac aacgatgatc
     7741 accgcccccg agccggatgt acgtctccca gcgccgatcg gccttcacct tcaccggctt
     7801 gtcgaaccgc gtcgccgggc tgacgtagaa cgccagcacc ccgcggccct cgaagtcgta
     7861 gagcttggcg tggacgtcga tcgggcggga gaacttttcc ggacttcggc aggtcccgag
     7921 aaagctgttc tgcatcccgt ccgggtcccc gacgccgacg atctcgaagg agccccgacg
     7981 ctccgcgacg ccgaaaacga gccaaccgcc cgaggagttc gcgaaggccg agaccgtcct
     8041 gtaggaatcc tctggaatcc cgccctgcgc ggccttcacc tcgaagtcgt cccactcgta
     8101 gccccgaagt cgatccagca gctcttcccg gtccatgcga tcagtcctga tccgtccggg
     8161 tcaccgcgac gtcgtaatag acgaggagct tctcgtcttc ctggaggact gactccggga
     8221 gcttggcggc gacggcgatg atcgtgccgt agtccttggc ctggaaggcc gccttgaagc
     8281 cggcgcggat cgcctcggtc cggaaggact tcagcttccg ctccttcgct tcccgatagc
     8341 tctcgaactc gcgcaggagg gcccggtcgc ggactctctc gaggtcggct tccttcttct
     8401 cgtcggggac gtaccacctg tcgacggccc tgtccctgag cctcggatcg tccttctcga
     8461 gattccgaca gtccttgtag ttgctgctga ggtacgagtg gatcttctcc ggaaccagac
     8521 ccttcccgtc gtaacagagg aagttctggg ccaggatctc cttcagctca accgtcttct
     8581 cgtgccgggc ccaggcctgc agctcgcgcg tgtagagcgg atggagttcc tggtaggtct
     8641 tcggcatctc ggtgaggatg ctccggagcc agaggatcgc gttcgactcg tcgttgaccg
     8701 agagctcgat ctgcttcagc ttcttcacgg ccgcccgctt cttgtcgtac tcgacgacct
     8761 gcgttggcag gaagaacatg ccgtcccgct ccgggaaccg ggctcgcagc ccggcgagga
     8821 agtcggccgt cccgaggggg atgctcagtt cccgctggac gtggaatgcc accatccggt
     8881 cgtgcagcat ctgcggcgtc cgctccgcga gaacctccgc ttcttcctta accgagacga
     8941 acaccgggac gttcgttagg tgctccgtga cgagggacca gaccgcctcc tcggaagagg
     9001 atccgagcga gaacttcttt gtcaccgctt cggtgggctt gtaggccgag atgacgagat
     9061 cctgcttcac ggccgtgctg gtgacctggc gataagaacc cgttccctta tcgagagtcc
     9121 gcacgtcggc gaccacgaac ccggcgcgcc cgagggcttc ctgtatcgcc cgccagaccg
     9181 cattagagga gttgctgaag actacggtca tccaccgccc aggcttcaag acccggtagt
     9241 actccgagaa acaccggcgc atcagatcct ggtactcgtg aagccccttg ttcttcggtg
     9301 cgtcgacgat ggcctctggg cctgtattag tcagaacccg atgccatgac tcgacgagga
     9361 ggttcaagtc tgagtacgga atgttctcgc cgaagggagg atccgtgaaa acgtagtcga
     9421 tcgagtcctg acggaggccg aggcgttcgc aggtcgaagt ctccacgacg gcatgtccgc
     9481 cgcggccgga gaacgccgag aacgccttct tggttctcgt gatcttcccg cccagcaggt
     9541 atcccggaga gacgtcgacg acttgggaac caacgtagta aacaccgttg aggtactggt
     9601 tgacttggga gtaatgagtc ggtgcatagc gtgcgagcac ggacataccc caaacggcct
     9661 gctccacgaa gaacttgttc cacctctcgg cagtgcggtc cgttccactc cttgctctct
     9721 tccagaggcc cgcgagcgtt tgggccggtc gtgcaaggaa caaatggtga atggccttga
     9781 accccttcgg cccgagtcgg gtcccgtggt acatctgctc cagcgggatc gcggtcgtcg
     9841 gaacctcagc cggaagcggg aggccaccga tcctttctag tattgcgagg tcgccatgat
     9901 cgggtttctt ctcgaacgtc ttcgccccga cgtcgtagtt gatgatgacc ggaacgaact
     9961 tgattcgctt ccacggccgc cgcgtggctg gatcggggac ggtctcgaag agctgctcaa
    10021 gcaggtcctt gctaaccttc gccccgcatc ccgggcatga aaactcgtcc ttcacctttt
    10081 tcgtcttctt gtcgagcgcc tgctcgacga agacgacctc cccgctgcac tccgggcagg
    10141 cgaagacctc gctccagacg gtgaagttga tccggccttt ccttccgtcc ttgtgggtcg
    10201 tctcgtacat ccagccgagc tcggcctcga cttcgtcgag gatgcggcgg gcctctctct
    10261 cgaacgcgcg cacgtcgaag ggcaggttgt agccggcggt gatgaaggtc gcggctggcc
    10321 cgagatcgtt caggatcgcg cgccgcgccc cccagtccgg cttcgtccgg ccctcggcct
    10381 tccactgcgc ctccagctcg cgccggtagt cggccggcgc ggcgccacac cattgcgccg
    10441 ccacgccggt catccccgac ccgcagaaac cgtcgaggac caggtcaccg gacttcgtgt
    10501 agtggaggat cgacgggacg atggcgaggt gcgggacctt ggtgtgatag ccgtgagcct
    10561 tgtagagcgc gtccgtcttg ccgacagtcg tgtcaacggc gaacggctcc cggtggtact
    10621 tctccgtcgg gtcgaaggga cgcccgtgaa agcgaacgaa gtcctcgaga aacgggttcg
    10681 ggcaggcggt gtaatagggc gggtccgaca ttcggagaat gtcttcgtcc tcgccgatcg
    10741 gaaacccctc gatcttccgg aagtcggggt ctctcagctt ctcgcgcagc tttccgagga
    10801 agtactcaag ccgggcctcg tcgctcggga aggtcatccc gaggcattca accggtttgg
    10861 cctcgagggc ttcctgctcg accgactttt caaagaactc cgtctgcccc gtggcatcga
    10921 gaaacaagcg gcggctcgcg gactgagatc gtttcggggt cttcgccacg gggttctcct
    10981 tgcttcgctg gctactcgag gacgattcgg accttcgagg ggtcctttcc gcggcacgca
    11041 tcggcgagga aggagtcgag tcgttccttc aggtcggtga gcgtgcttgc ggcgccacct
    11101 ttcccaaggg cggcgacgag ggcctctcgg gccagcgcca ctctctgaag accggagaag
    11161 acctcctgga gagccttcac gaggtgactg tccacgggct cgg
//
LOCUS       DAOONL010000107        11158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6581_length_11158_cov_20.089255, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000107 DAOONL010000000
VERSION     DAOONL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6581_length_11158_cov_20.089255"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2980)
                     /locus_tag="PLP50_13295"
     CDS             complement(<1..2980)
                     /locus_tag="PLP50_13295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA52567.1"
                     /translation="MRPPRPLRPGSRRAALVLAGLLAFAPGADAEEAAASGTTPAGRT
                     TFRAYGVERGLGDFLILTLYQDRRGFLWAGTEDGLFRYDGREFERFGRTEGLPSTYVG
                     AMCEGADGTLWAGTFSGLARWADGAFVPVAGGAGLPAEPVRDLQGTPDGRLWVGQRSG
                     LYFGTSARGFFPAPGWPGGDASALAVDGQGRLWAAASPAAEGGRTRVLTLSDSGWREW
                     PLPGLAGERLTALAGDGAGRVWARSSTQLFVLDPGADAFRQARPPARVESQLGRLAVD
                     RDGRILVPTETGLLVLYEDGWHLVGRDQGLPTGWAQVALVDREGSLWIGSTGLHRQLG
                     GGVWHGCTTVDGLPSDVVRALFRSADGTLWVGTEKGLARAAASRFELLAGTERNGIRT
                     IQEDRAGSLYLAGVPVEVLRVDPRRGRIERFGAESGLRGTRVLRMVLDPSGTLWVAAD
                     GGGLRAARTGPGPLRFEPVVVPGGSETEYFSGLILDGKGRLWAAGEKGLAVRDGGTWT
                     RFTNADGLRETHVAYALSLRSGELVVAYFEGAGLSRVTYDGGRISVADLLAPGSVLAT
                     QKVFMLGEDRSGRIWAGTGLGVTSVENGAERRYRAEDGLVGEDFNNMTFLEDADGSLW
                     FGTMSGLARFDPRVTPPSVPPPATAILETKLGPRSWKGPAPDGERLVYRENVLEVRYA
                     GLSFVRENAVESETRLVGLEPEWRASRIRELRISALPPGEYRFEVRSRAAAGEWGPVA
                     GFSFSIAPAWWQSLWFRALALLALAGLVALAVQRRLRKLHRRTVILETLVDERTRELK
                     AANEALRNQSLTDPLTGLRNRRYLGVSVPEVVAQVNRNHRNVALGRGDRATVNVDLVF
                     LMVDVDHFKSVNDAHGHHAGDEVLRQLAAVLRSATRESDTVVRWGGEEFLVVGRNTCR
                     KECAVLAERIRARVAAHEFRLDDGTVLRRTCSVGFASYPVVGSTPDHVEWEQVVDLAD
                     QALYAAKLGGRNAWVGLVARD"
     gene            complement(3071..>3250)
                     /locus_tag="PLP50_13300"
     CDS             complement(3071..>3250)
                     /locus_tag="PLP50_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPA52568.1"
                     /translation="LSMKPGLTGLWQVSGRSDLPSFDQWMELDLAYIDNWSLTLDAKI
                     LLRTIPTVLSGRGAR"
     assembly_gap    3251..3350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3351..4375)
                     /locus_tag="PLP50_13305"
     CDS             complement(<3351..4375)
                     /locus_tag="PLP50_13305"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPA52569.1"
                     /translation="MLKQQARSVAASLYAADLASTLAALAAAYIVRSELLPRFFPERL
                     TPLYPFTWYLVLVGPVVVIWTGLLFLAGAYRSRRTSGLKDEALLVGKIVLGGTVLLTI
                     LVYGLRLDFISRPFLPLFAVLNTLFLVVERLTIRIVARRVRARGYNYRTVVIIGDTPR
                     ARSMARLIHDHPWWGLKLLGLVRERPASSEAGTTAGGLPVLGSLEDFPTILTSLPVDE
                     VILAVDRGDVDQLEDVFLMCEEMGVKTRLILDFFPHVFAKVELEEFDGTPLLTFSTTP
                     AETGALLVKRAVDVGLALLLGIVCLPLAALLAVLIKLSSHGPVLFRQVRCGLSGRPFT
                     FYKLRTMT"
     gene            complement(4369..5502)
                     /locus_tag="PLP50_13310"
     CDS             complement(4369..5502)
                     /locus_tag="PLP50_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HPA52570.1"
                     /translation="MRLAILGTRGVPAAYGGFETLAEELSARLAARGHDVTVYARRGA
                     VREEVVSWRGARIVFTPTLRHKYLDTVVHGVTSGLHAAAAGYDAVLVCNGANALACRL
                     PRLLAAGTRVLLNVDGLERNRRKWNRLGKAVYALSERLSCVLPDVVVTDAEEIRRYYR
                     ERYGAESAFVPYGSDLPEPEDATVLGRLGLEPGQYLLYVSRFEPENNPDAVVRAYRDV
                     PGERPLLLVGSAPYAEGFIARLRDEAARDPRVVLPGAIYGEGYRALLANAAAYVHATE
                     VGGTHPALIEAMGYGRPVLVHDTPENREVAGEAALYWDARAPETLARLLGGLLPDAGR
                     RSALGAAARRRAAERYRWDDVTDAYEALLSGRDPDGLRSAPEC"
     gene            complement(5499..6530)
                     /locus_tag="PLP50_13315"
     CDS             complement(5499..6530)
                     /locus_tag="PLP50_13315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA52571.1"
                     /translation="MPRVVVSLVTHDESRDTERLLPTLFAQTFRDFSVVAVDNRSEDG
                     TRSALAAAQKTAPVPMEIVASRENLGFAGGHNIGIAKAIEREAAWVLVLNADVVLAPD
                     YLERLLVDADRPGREWVGAMTGKILRADGPELEPTTVIDTVGIRMTRGGRHFDVGAGE
                     PDTGRWDRPAEIFGVSGCVALYRAAALADVKISTGYFDDDFFVYREDVDLAWRLRGRG
                     WSARCVPSALAWHRRRNLPERRRQMSAVANLHSVKNRFLLRINNAGPDHLRATFPLTF
                     ARDVVVVGACAFAERTSLEALRWLARNRPRLLAKRREVLERRTVSDRALLRWFGPDPG
                     GARIPDVDA"
     gene            complement(6531..7490)
                     /locus_tag="PLP50_13320"
     CDS             complement(6531..7490)
                     /locus_tag="PLP50_13320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52572.1"
                     /translation="MTGPLSRVRAIVVSWNSAGHVGRAVASVPPPARVVVVDNASTDG
                     SASEAERAGAVVLRLESNAGFGPACNLGAGPVDGLPDAETLLFLNPDAALVDGPAALA
                     ALLAELDADPAVAAVAPALEGDGQEEFQLRRLPTLGSLAREAFLVNRLFASNRGFRGE
                     RYLDRDRSEPFDVEQPAAAALLVRREAFERVGGFDPAFAPAWFEDVDLSAKLLESGYR
                     IRFVPAARASHVGGTTMRALPYDDYLPLYTRNLIRYLGRHSGPATRAGARSVLAAGAL
                     LRLVLLPFVRGDHARPEAARAYVRVLRGLLGLGFRSALLPGGA"
     gene            complement(7487..8410)
                     /locus_tag="PLP50_13325"
     CDS             complement(7487..8410)
                     /locus_tag="PLP50_13325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52573.1"
                     /translation="MERLPVGVVVVNWNGGSLLDACLDALAKEPASRVLVVDNGSDAE
                     ELRRLSARPGVFVLPLGANRGFASAANGGAIDARLKALPYLAFVNNDAVLEPGYLAAC
                     IGALEADAGLAAVQGVILDGEGRLADGLGIAWNRRLEAVQLGHGEPPPAADAPVFPVP
                     GVSGTAPVFRRRAFEEAGGFAGSFFAWYEDADLALRLLRSGARFACVPAARARHAGSA
                     TGRRIPERKWRLLLANRLRTLRRNLSAGARLSAFLAHPVPLPALRDAVRELGAGRTLR
                     TLAAATAAMTRRSAEDRAARDGPPLLRSLPR"
     gene            8448..9734
                     /gene="serS"
                     /locus_tag="PLP50_13330"
     CDS             8448..9734
                     /gene="serS"
                     /locus_tag="PLP50_13330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPA52574.1"
                     /translation="MLARDFLRTRFADLPHLLRNRTYDAGALARWGELDAERRRLLTS
                     VDALKAERNTLSAEVGRKKKAKEDATAEMERARALGGEVSAGEKRLEEIEGEFFAIEQ
                     TLPNVPDESVPDGEDETGNVVLKSWGEPARFDFAPKPHFELGPALGILDFERAAKVTG
                     SRFTVLSGEASLLSRALIAFMLDLHTREHGYREVLPPFIVNAASLFGTGQLPKFEADL
                     FRLKDTDWYLAPTAEVPVTNLHRDEVLREEQLPISYCAYTPCFRAEAGAAGKDTRGLI
                     RQHQFDKVELVKFTKAEESAEAHEKLTRDAETVLERLGLPYRRVVLCRGDMGFSSAKT
                     YDLEVWLPGQDAYREISSCSDFGAFQARRAAIRVKRTVDGKGRNEYVHTLNGSGLAVG
                     RTLVAILENYQREDGSVAIPEALRPYCGGLAEIRKG"
     gene            9813..9905
                     /locus_tag="PLP50_13335"
     tRNA            9813..9905
                     /locus_tag="PLP50_13335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9847..9849,aa:Ser,seq:gct)
     gene            9917..10004
                     /locus_tag="PLP50_13340"
     tRNA            9917..10004
                     /locus_tag="PLP50_13340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9951..9953,aa:Ser,seq:tga)
     gene            10054..10971
                     /locus_tag="PLP50_13345"
     CDS             10054..10971
                     /locus_tag="PLP50_13345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA52575.1"
                     /translation="MAQIRSRILIVDDDALSRRVLEQVLVAADYEVESAGDGQEALGK
                     VRLHRPDVVVTDWQMPNMDGLTLCRILKGTEETRFTHVIMMSTQGETEAKVTGLETGA
                     DDYLVKPVEPVELKARVKVGLRLQRALSDLASKNEILEKLALTDPLTGLSNRRAFGEA
                     LASELSRIERHNQPASLLAFDLDHFKRVNDSHGHAVGDEVLAAFAAVLKRGCRRGDLA
                     ARLGGEEFAILLPTTALVPAALVAERIRRATECRPLGVSVPVPVTVSVGVASTEGLPL
                     PPAAAELLRLADGALYRAKAEGRNRVVSG"
BASE COUNT         1626 a         3896 c         4169 g         1367 t
ORIGIN      
        1 cgtcccgggc gacgaggccg acccaggcgt tccgccctcc gagcttcgcg gcgtagaggg
       61 cctggtcggc gaggtcgacg acctgctccc actcgacgtg gtccggcgtc gacccgacca
      121 ccgggtacga ggcgaagccg accgagcagg tccggcgcag gaccgtcccg tcgtcgaggc
      181 ggaactcgtg cgccgcgacc cgcgcgcgga tcctctcggc caggacggcg cactccttgc
      241 ggcacgtgtt gcgcccgacg acgaggaact cctcgcctcc ccaccggacg acggtgtcgc
      301 tctcgcgcgt cgcggagcgg aggaccgcgg cgagctgccg gaggacctcg tccccggcgt
      361 ggtggccgtg ggcgtcgttg acggacttga agtggtcgac gtcgaccatc aggaagacga
      421 ggtcgacgtt gacggtggcg cgatcgccgc ggccgagggc aacgttccgg tggttccggt
      481 tcacctgcgc gacgacctcg gggacggaga cgccgaggta gcgccggttc cggaggcccg
      541 tcagcgggtc ggtcaggctc tggttccgga gcgcctcgtt cgcggccttc agctcgcggg
      601 tcctctcgtc gacgagcgtc tcgaggatca ccgtccgccg gtggagcttc cggaggcgac
      661 gctggacggc gagcgcgacg aggcccgcca gggccaggag cgcgagggcg cggaaccaga
      721 ggctctgcca ccaggccggg gcgatcgaga acgagaagcc ggcgacgggg ccccactcgc
      781 ccgccgccgc gcggctccgc acctcgaacc ggtactcgcc cggagggagc gccgagatgc
      841 ggagctcccg gatgcgcgag gcgcgccact ccggctcgag gccgacgagc cgggtctcgc
      901 tctcgacggc attctcgcgg acgaagctca ggccggcgta acgcacctcg aggacgttct
      961 cccggtagac gaggcgctcg ccgtcgggag cgggcccctt ccaggagcgt ggcccgagct
     1021 tcgtctcgag gatcgccgtc gccggcggcg ggacggaggg gggcgtcacg cgggggtcga
     1081 accgggcgag gccggacatc gtcccgaacc agaggctccc gtccgcgtcc tccaggaacg
     1141 tcatgttgtt gaagtcctcg ccgacgagcc cgtcctcggc gcggtagcgg cgttcggccc
     1201 cgttctcgac ggaggtgacg ccgaggcccg tgccggccca gatccgcccc gaacggtcct
     1261 cgccgagcat gaagaccttc tgggtcgcga ggacgctccc gggcgcgagg aggtcggcga
     1321 cggagatccg cccgccgtcg tacgtcaccc ggctcaggcc cgcgccctcg aaatacgcga
     1381 cgacgagctc gccgctccgg agcgagaggg cataggcgac gtgcgtctcg cggagcccgt
     1441 ccgcgttggt gaagcgcgtc cacgtcccgc cgtcgcggac ggcgaggccc ttctctccgg
     1501 cggcccagag gcgtcccttc ccgtccagga tcaggccgct gaagtactcg gtctcgctgc
     1561 ccccgggcac gacgaccggc tcgaagcgaa gcggtcccgg tcccgtccgc gcggcgcgga
     1621 ggcctccgcc gtcggccgcg acccagagcg tgccggaggg gtcgaggacc atccggagga
     1681 cgcgcgtccc gcggagcccc gactcggcgc caaaccgttc gatccgtccg cggcgcgggt
     1741 cgacgcgcag cacctcgacg gggactccgg ccaggtagag gctgccggcg cggtcctcct
     1801 ggatcgtccg tatcccgttc cgctcggtcc cggcgaggag ctcgaaacgg ctcgctgccg
     1861 cccgggcgag acccttctcc gtcccgaccc agagcgtccc gtcggcgctc cggaagaggg
     1921 cgcggacgac gtcgctcggg aggccgtcga ccgtcgtaca gccgtgccac acgccgccgc
     1981 cgagctgcct gtggaggccc gtgctcccga tccagagcga cccctcgcga tcgacgaggg
     2041 cgacctgcgc ccatcccgtc ggaagcccct gatcgcggcc gacgaggtgc cacccgtcct
     2101 cgtacaggac gaggagcccc gtctccgtgg gcacgaggat gcgaccgtcg cggtcgacgg
     2161 cgaggcggcc gagctggctc tcgacgcggg cgggaggccg cgcctgccgg aacgcgtcgg
     2221 cgccgggatc gaggacgaag agctgcgtgg acgaacgtgc ccagacgcgg ccggccccgt
     2281 cgcccgcgag cgcggtgagc cgttcgcccg cgagacccgg cagcggccac tcgcgccagc
     2341 ccgaatccga gagcgtcagg acccgcgtcc gccccccttc cgcggcggga gaggccgcgg
     2401 cccagagacg cccctggccg tcgaccgcga gggcgctcgc gtcgccgccg ggccagcccg
     2461 gcgcgggaaa gaagccgcgc gcgctcgtcc cgaagtagag cccgctccgc tggccgaccc
     2521 agagccgccc gtccggggtc ccctgcagat cgcgcacggg ttcggcggga aggccggcac
     2581 cgccggcgac cggcacgaag gcgccgtccg cccaccgggc gaggcccgag aacgtcccgg
     2641 cccagagcgt cccgtcggct ccttcgcaca tcgcgccgac gtacgtggag gggagcccct
     2701 cggtccgtcc gaaccgctcg aactcgcggc cgtcgtagcg gaagagcccg tcctcggtcc
     2761 ccgcccagag gaagccgcgc cggtcctggt agagcgtcag gatgaggaag tcgccgaggc
     2821 cacgctcgac gccgtaggcg cggaacgtcg tccgtccggc gggggtggtc ccggacgcag
     2881 ctgcctcctc cgcgtcggcg ccgggcgcga acgcgaggag gccggcgagg acgagtgcgg
     2941 cgcgacggga gccgggacgg agcggtcgag gcggacgcat gcaggtctcc tccgcgcgcg
     3001 gaacggaccg gagccggaaa ggccccccac cagtctagtc tccgcgccga ggagccgggg
     3061 cgccgatccc tcagcgcgcg ccgcggccgc tgaggaccgt cgggatcgtc cggaggagga
     3121 tcttcgcgtc gagcgtgagc gaccagttgt cgatgtaggc gaggtcgagc tccatccact
     3181 ggtcgaagct cgggaggtcg ctccggccgg agacctgcca gagcccggtc aggccgggct
     3241 tcatcgagag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtcatcgtcc
     3361 ggagcttgta gaacgtgaag gggcggccgc tcaggccgca gcgcacctgc cggaacagga
     3421 cggggccgtg cgagctcagc ttgatcagga cggcgaggag ggcggcaagc gggaggcaga
     3481 cgatgccgag gaggagcgcg agcccgacgt cgacggcgcg cttcacgagg agcgcgccgg
     3541 tctcggcggg ggtcgtcgag aaggtgagga gcggcgtgcc gtcgaactcc tcgagctcca
     3601 ccttcgcgaa gacgtggggg aagaagtcga ggatgagccg cgtcttcacc cccatctcct
     3661 cgcacatcag gaagacgtcc tcgagctggt cgacgtcgcc gcggtcgacc gcgaggatca
     3721 cctcgtcgac gggcagggac gtcaggatcg tcgggaagtc ctcgagggag ccgaggaccg
     3781 gaaggccgcc cgccgtcgtc ccggcctccg aggaggcggg acgctcgcgg acgaggccga
     3841 ggagcttcag cccccaccag gggtggtcgt ggatgagccg cgccatcgac cgggcgcgcg
     3901 gcgtgtcgcc gatgatcacg accgtccggt agttgtaccc gcgggcgcgg acccgccgcg
     3961 cgacgatccg tatcgtcagc cgctccacga cgaggaagag cgtgttgagg acggcgaaga
     4021 gcgggaggaa cggccgcgag atgaagtcga gcctcaggcc gtagacgagg atcgtcagga
     4081 ggacggtccc gccgaggacg atcttcccga cgaggagcgc ttcgtccttc aggcccgagg
     4141 tgcgccggct ccggtaggcg ccggccagga agaggagacc ggtccagatg acgacgaccg
     4201 gcccgacgag gacgaggtac caggtgaacg ggtagagggg cgtcagcctc tcggggaaga
     4261 agcgggggag gagctcgctt ctgacgatgt aggccgcggc gagggccgcg agcgtcgagg
     4321 ccaggtcggc ggcgtagagc gaggcggcga cggacctcgc ctgctgcttc agcattccgg
     4381 cgccgatcgt agcccgtccg ggtcccgccc ggacagaagc gcttcgtagg cgtcggtcac
     4441 gtcgtcccag cggtagcgct ccgcggcgcg gcgacgggcc gccgcgccga gcgccgagcg
     4501 ccgcccggcg tcggggagga ggccgccgag gagccgcgcg agcgtctctg gcgcgcgggc
     4561 gtcccagtag agggcggctt ctcccgccac ctcgcggttc tcgggggtgt cgtggacgag
     4621 gaccggacgt ccatagccca tcgcctcgat cagcgccggg tgcgtcccgc ccacctcggt
     4681 cgcgtggacg tacgccgccg cgttcgccag gagcgcccgg tatccctctc cgtagatcgc
     4741 gccggggagg acgacgcggg ggtcccgcgc cgcctcgtcc cggaggcggg cgatgaaccc
     4801 ctcggcgtac ggcgccgacc cgacgaggag gaggggccgc tcgcccggga cgtcccggta
     4861 ggcgcggacg acggcgtcgg ggttgttctc cggctcgaag cgcgagacgt agaggagata
     4921 ctgccccggc tcgagcccga gtcgcccgag gaccgtcgcg tcttccggct cggggaggtc
     4981 ggagccgtac gggacgaacg ccgactcggc gccgtaccgc tcccgatagt agcgccggat
     5041 ctcctcggcg tcggtcacga cgacgtccgg gaggacgcac gagagcctct cggagagcgc
     5101 gtagacggcc ttcccgaggc ggttccactt cctccggttc cgctccaggc cgtcgacgtt
     5161 caggaggacc cgcgtcccgg ctgccaagag ccgcgggagc cggcaggcga gcgcgttcgc
     5221 gccgttgcag acgaggacgg cgtcgtaccc ggcggcggcc gcgtgaagcc ccgacgtcac
     5281 gccgtgaacg acggtgtcga ggtacttgtg acggagcgtc ggcgtgaaga cgatgcgggc
     5341 gccgcgccag gaaacgactt cctcacggac ggcgccgcgc cgggcgtaga ccgtcacgtc
     5401 gtgcccccgc gcggcgagcc gggccgagag ctcctcggcg agcgtctcga agcctccgta
     5461 cgccgcgggg acgccgcgcg tcccgaggat cgcgagcctc acgcgtccac atccgggatg
     5521 cgcgcccctc ccgggtcggg tccgaaccag cggagcagcg cccggtcgga gaccgtccgt
     5581 cgctcgagga cttcgcggcg cttggcgagg agccggggtc ggttccgcgc gagccagcgg
     5641 agcgcttcga gcgaggtcct ctccgcgaag gcacaggccc cgacgacgac gacgtcccgg
     5701 gcgaacgtga gggggaacgt cgcgcggagg tggtcggggc cggcgttgtt gatccggagg
     5761 aggaagcggt tcttcaccga gtggaggttc gcgacggccg acatctgccg tcgccgctcc
     5821 gggaggttcc ggcgccggtg ccacgcgagc gccgaaggga cgcagcgggc cgaccagcct
     5881 cgtccgcgga ggcgccaggc gaggtcgacg tcctcgcggt agacgaagaa gtcatcgtcg
     5941 aagtagccgg tcgagatctt cacgtccgcg agggccgcgg cgcggtagag ggcgacgcag
     6001 ccggagacgc cgaatatctc ggcgggccgg tcccaccggc cggtgtcggg ctctcccgcg
     6061 ccgacgtcga agtgccgtcc cccacgggtc atccggatcc cgaccgtgtc gatcacggtc
     6121 gtcggctcga gctccggacc gtcggcgcga aggatcttcc cggtcatcgc gccgacccac
     6181 tcccggccgg gccggtcggc gtcgacgagg agcctttcga ggtagtcggg agcgaggacg
     6241 acgtcggcgt tcaggacgag gacccacgcg gcctcccgct cgatcgcctt cgcgatcccg
     6301 atattgtgtc ctccggcgaa gccgaggttc tcgcgggagg cgacgatctc catcggcacc
     6361 ggggcggtct tctgcgcggc cgcgagcgcg gagcgggttc cgtcctccga gcggttgtcg
     6421 acggcgacga cggagaagtc gcggaacgtc tgggcaaaaa gcgtcgggag gagccgctcg
     6481 gtgtcgcgcg actcgtcgtg cgtgacgagg gagacgacga cgcgcggcac tcaggcgccc
     6541 ccgggaagga gcgcgctccg gaagccgagg cccaggaggc cgcggaggac gcgaacgtac
     6601 gcccgcgccg cctccggccg ggcgtggtcg ccgcggacga acgggaggag gacgaggcgg
     6661 aggagcgccc ccgcggcgag gacggaccgc gccccggcac gcgtggccgg cccggaatgg
     6721 cgccccaggt accggatgag gttccgcgtg tagagcggca ggtagtcgtc gtaggggagc
     6781 gcgcgcatcg tcgtcccgcc cacgtggctc gcgcgagccg cggggacgaa gcggatacgg
     6841 taccccgatt cgaggagctt cgccgagagg tccacgtcct cgaaccaggc gggggcgaag
     6901 gccgggtcga agccgcccac ccgctcgaac gcctcgcgcc ggacgaggag cgcggcggcc
     6961 gcgggctgct cgacgtcgaa cggctccgag cggtcgcggt cgagatagcg ctccccgcgg
     7021 aagccccggt tcgacgcgaa gaggcgattc acgaggaacg cctcccgggc gagggagccg
     7081 agcgtcggga gccttcgcag ctggaactcc tcctgcccgt ctccctcgag ggcgggcgcg
     7141 acggccgcga cggccgggtc ggcgtccagc tcggcgagga gcgcggcgag cgcggcgggg
     7201 ccgtcgacga gggcggcgtc ggggttcagg aagaggagcg tctcggcgtc ggggagcccg
     7261 tccacgggcc cggccccgag gttgcacgcc gggccgaagc cggcgttcga ttccagccgg
     7321 aggacgacgg cgcccgcccg ctccgcctcg gaggccgagc cgtcggtcga ggcgttgtcg
     7381 acgacgacga cccgcgccgg cgggggaacc gacgcgacgg cccggccgac gtgcccggcc
     7441 gagttccagg agacgacgat cgctctcacc cgggagaggg ggcccgtcat cgtggcaggc
     7501 tccgcaggag agggggtccg tcgcgcgcgg cgcggtcctc ggccgagcgt cgcgtcattg
     7561 cggccgtggc ggcggcgagc gtccggaggg tgcgccccgc gccgagctcg cggacggcgt
     7621 cgcggagggc ggggagcggc accgggtggg cgaggaaggc ggagagacga gcgcccgccg
     7681 agagattgcg gcgaagcgtc cggaggcggt tcgcgaggag cagccgccac ttccgctcgg
     7741 ggatccggcg tcccgtcgcc gagccggcgt gacgggcccg ggcggcgggg acgcaggcga
     7801 agcgggctcc ggagcgcagg agccggaggg cgaggtcggc gtcctcgtac caggcgaaga
     7861 aggagccggc gaagcctccg gcctcctcga acgcccgccg ccggaagacg ggcgccgtgc
     7921 cggagacgcc ggggaccggg aagacgggcg cgtcggcggc cggcggcggc tccccatggc
     7981 cgagctggac ggcctcgagg cgccggttcc acgcgatccc gagaccgtcg gcgaggcgcc
     8041 cttccccgtc caggatcacg ccctggacgg cggccaaccc cgcgtcggcc tccagcgcgc
     8101 cgatgcacgc ggcgaggtac cccggctcga ggacggcgtc gttgttgacg aacgcgaggt
     8161 acggcagcgc tttcagccgg gcgtcgatcg ctccgccgtt cgccgcggag gcgaagccgc
     8221 ggttcgcacc gagcgggagg acgaagacgc cggggcgtgc cgagagccgg cggagctcct
     8281 ccgcatcgga gccgttgtcg acgacgagga cccgcgacgc cggctctttc gcgagcgcgt
     8341 cgaggcaggc gtcgaggagc gagcctccgt tccagttcac gacgacgacc ccgaccggga
     8401 gcctctccat ccggccgatt atcggggcgc cgtaaaatcc gcctcccatg ctggcccgag
     8461 acttccttcg cactcgtttc gccgacctgc cgcacctcct ccggaaccgg acgtacgacg
     8521 ccggcgccct cgcccgctgg ggcgagctcg acgccgagcg ccgcaggctc ctgacgtccg
     8581 tcgatgcgct gaaggccgag cggaacacgc tctcggccga ggtggggcgg aagaagaagg
     8641 cgaaggagga cgcgaccgcc gagatggagc gggcgcgggc cctcggcgga gaggtctccg
     8701 ccggagagaa gcggctcgag gagatcgagg gggagttctt cgcgatcgag cagaccctcc
     8761 cgaacgtccc cgacgagagc gtcccggacg gcgaggacga gacggggaac gtcgtcctga
     8821 agagctgggg cgagccggcg cgcttcgatt tcgcgccgaa accgcacttc gagctcggcc
     8881 ccgcgctcgg catcctcgac ttcgagcggg cggcgaaggt caccggctcc cgcttcacgg
     8941 tcctctcggg cgaggcctcg ctcctctctc gggcgctgat cgcgttcatg ctcgacctcc
     9001 atacccgcga gcacggatac cgggaggtcc tgcccccgtt catcgtgaac gcggcgtcgc
     9061 tcttcgggac gggccagctc ccgaagttcg aggcggacct cttccgcctg aaggacaccg
     9121 actggtacct cgccccgacg gcggaggtcc cggtcacgaa cctccaccgc gacgaggtcc
     9181 tccgcgagga gcagctcccg atctcctact gcgcctacac cccgtgcttc cgcgccgagg
     9241 cgggggcggc ggggaaggac acgcggggcc tgatccgcca gcaccagttc gacaaggtcg
     9301 agctcgtcaa gttcacgaag gcggaggaat cggcggaggc gcacgagaag ctgacgcgcg
     9361 acgcggagac cgtcctcgag cgcctcggcc tgccgtaccg gagggtcgtc ctctgccgcg
     9421 gggacatggg cttctcctcg gcgaagacct acgacctcga ggtctggctg ccggggcagg
     9481 acgcctaccg cgagatctcc tcctgctccg acttcggcgc cttccaggcc cgccgcgcgg
     9541 ccatccgggt gaagcggacc gtcgacggga agggacgcaa cgagtacgtc cacacgctca
     9601 acgggtcggg gctcgcggtg gggcggacgc tcgtggcgat cctcgagaac taccagcggg
     9661 aggacggctc ggtggcgatc ccggaggcgc tccggcccta ctgtggcggg ctcgccgaga
     9721 tccggaaggg ctgagcgtcc gtcccggata gcccgatgag gccccgccgc ccgtcgcggg
     9781 gcctctgcta aactccgccc cccgcgggaa gcggacaggt gggtgagtgg cttaaaccaa
     9841 cggtttgcta aaccgtcgta ggccttcaaa gcctaccggg ggttcgaatc cccccctgtc
     9901 cgccagctcc ttcgacggag gagtggccga gtggtttatg gcggcggtct tgaaaaccgt
     9961 tgagctgcaa ggctccgggg gttcgaatcc ctcctcctcc gccattccga cccccgtggg
    10021 agaatcgacg gtcccgcctt cgggaccccc ctgatggccc agatccgctc ccgcatcctg
    10081 atcgtcgacg acgatgccct ctcgaggcga gtcctggagc aggtgctcgt ggcggcggac
    10141 tacgaggtgg agagcgccgg ggacgggcag gaggcgctcg ggaaagtccg cctccaccgc
    10201 cccgacgtcg tcgtcaccga ctggcagatg ccgaacatgg acggcctcac cctctgccgg
    10261 atcctgaagg ggaccgagga gacgcgcttc acccacgtca tcatgatgtc gacgcagggg
    10321 gagacggagg cgaaggtgac cggcctcgag acgggcgccg acgactacct cgtcaagccg
    10381 gtcgagccgg tggagctgaa ggcgcgggtg aaggtcgggc tccgcctcca gcgcgccctc
    10441 tcggacctgg cttcgaagaa cgagatcctc gagaagctcg ccctgaccga cccgctcacc
    10501 ggattgtcga accgccgcgc tttcggagag gcgctcgcct cggagctctc gcggatcgag
    10561 cggcacaacc agccggcgag cctcctcgcg ttcgacctcg accacttcaa gcgggtgaac
    10621 gactcccacg ggcacgccgt gggggacgag gtcctcgccg ccttcgcggc ggtcctgaag
    10681 cggggctgcc gaaggggcga cctcgccgcc cgcctcgggg gagaagagtt cgcgatcctc
    10741 ctcccgacga cggccctcgt tcccgcggcc ctggtcgcgg agcggatccg gcgggcgacg
    10801 gaatgtcgtc cgctcggggt gagcgttccg gtccccgtga ccgtgtcggt cggggtcgcc
    10861 tccaccgaag gccttccgct tcccccggcg gccgcggagc tcctccggct ggccgacggc
    10921 gccctttacc gggcaaaggc cgagggacgg aaccgggtcg tctccggctg accggcccgg
    10981 gcgggcaaac ggctcgctcc cttcgatttc cgggcccggg cccgccgaaa cgggaaacta
    11041 ccggccccct tcgttcgtat ccctcgattg gcgccggccc acggcgccgg gcgaaaggaa
    11101 cgcgagagag atgagctccc ggctgaaatg gatcctcggg gcggtcgccg ccctcgtc
//
LOCUS       DAOONL010000108        10936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6769_length_10936_cov_18.681095, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000108 DAOONL010000000
VERSION     DAOONL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6769_length_10936_cov_18.681095"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..790
                     /locus_tag="PLP50_13350"
     CDS             236..790
                     /locus_tag="PLP50_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA52576.1"
                     /translation="MTRPPWTPPETIETPRLRLRRPRSDDAEAVFAYASDPVVTRLMD
                     WPTHLSIETSRRFLAYCETGWETGTEFTWLLTLPPSGRAVGAIACRPKERGVELGYVL
                     HRDVHGRGLATEASRAVVGWLKAVPDVVRIEATCDVENEASARVLEKAGLARERLLPA
                     YGARPNLGPEPRGALLFAWTRTAD"
     gene            823..1326
                     /locus_tag="PLP50_13355"
     CDS             823..1326
                     /locus_tag="PLP50_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HPA52577.1"
                     /translation="MSLSVFDDPGQPPGPAVLRRRLGPAATAWKALVDGVAERCGPVE
                     ELWSFGGAKFGWSLRLLRKGRVLLYLTPQEGRLLAGLVLGGKAVAAAREAGLPPALLA
                     QVEAAPQYAEGRGLRVEVSGPDGVEGLLLLVSLKADPSVAPSVTAASPSRAARPRGGG
                     GIRRGGR"
     gene            complement(1256..1849)
                     /locus_tag="PLP50_13360"
     CDS             complement(1256..1849)
                     /locus_tag="PLP50_13360"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPA52578.1"
                     /translation="MLEGTRCPWAGSDPLYVAYHDEEWGVPSRDERHLFEMLVLEGAQ
                     AGLSWLTVLRKREGYRRAFAGFDPGRVARFGPGDVERLLGDAGIVRNRLKIESAIDSA
                     RAYLALQKEAGSFASWLWAHVGGRPIQGAFREMRDVPAKTPLAESISKELRKRGFRFV
                     GPTIVYAYLQAVGVVNDHLVGCPRHRAVARLAKGSPR"
     gene            1911..2033
                     /locus_tag="PLP50_13365"
     CDS             1911..2033
                     /locus_tag="PLP50_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52579.1"
                     /translation="MRPIVRSVPAEAVLHGSLPGRIFRTLLAALLAASAVLLAG"
     gene            complement(2050..3180)
                     /locus_tag="PLP50_13370"
     CDS             complement(2050..3180)
                     /locus_tag="PLP50_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPA52580.1"
                     /translation="MNHGSFGACPVAVLERQAELRDELEREPVDFLVRRLPERLAEVR
                     AVLGGFLGAEPDDLAFVPNATTGVNAVLGSLDLNPGDELLTTTHAYPGCRNALAHACA
                     RTGARLVVADVPFPLEGEDEVVAPVLAAVSPRTRLALLDHVTSPTALVFPIERLVGAL
                     RERGVETLVDGAHAPGMVPVDLDRLGAGWYTGNAHKWLCSPKGAAFLHVREDLQTGAR
                     PAVTSLGYSEVASPARFRAEFDWTGTCDPTPWLTIPAALDALASLLPGGWSEVMTRNR
                     ALALEARDVLGETLRVSPAAPAEMIGSMASVPLPMATPGSVAARLGPQGLGLWLRERS
                     VETWLYPWPSPGGMLVRVSAQLYNAKAEYERLGALLAEALVR"
     gene            complement(3273..5333)
                     /locus_tag="PLP50_13375"
     CDS             complement(3273..5333)
                     /locus_tag="PLP50_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52581.1"
                     /translation="MPTNPRDARAVDPLVGVLTEIARTVTETLSLPEVFARVAGAARR
                     VLPFDAAGVVRLVEGPALLSWGLSTPGDVEGGRRYGRKDLSDVLWPGASSPRRIDDVA
                     ALFDATRQGDRELLEAGMRSMLVATIVRGDVPLGDLWFASRATGGFGPADEPTAQAIA
                     DILSATLEHERLAEEERRRVRRHRELDALAPALARALDVREVFDQVSAIAREVLPHDR
                     MTLGLLSEDRTENRMWAVSGDRDWVPDTYPIPDSERAQMVADYQIVCDAEADLPADSS
                     RRAILLAAGLRSSLRVPIRLEGKTVGALSFHAREVRSFREEDVDLALRVADQVALAFS
                     HQRLADEARRAEEARQEAARLEARVRALSDELDSRDGFGRVVGGSPAWRSVLVQASKV
                     APTETTVLLTGESGTGKEVVARAVHRASARSSGPFVALNCAALPEQLLESELFGYEKG
                     AFTGAYASRAGRLEQAAGGTLFLDEAGETSPLVQAKLLRVLEAREFQRLGGAKVLRAD
                     VRIVAATNRDLHAAMARGDFREDLFYRLSVFEIHLPPLRERPEDVLLLTERFLEDLSR
                     NVGRPAPGVSREARELLLAYSWPGNVRELRNALERAVILSEGGLVQVEHLPIAVARSS
                     AARVASAAAIELPPTGLDLDALEKTLVTQALERAKNNRSRAARLLGLTRAQLYTRLER
                     YGLA"
     gene            5376..6314
                     /gene="meaB"
                     /locus_tag="PLP50_13380"
                     /pseudo
     CDS             5376..6314
                     /gene="meaB"
                     /locus_tag="PLP50_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
     gene            6351..6752
                     /gene="mce"
                     /locus_tag="PLP50_13385"
     CDS             6351..6752
                     /gene="mce"
                     /locus_tag="PLP50_13385"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPA52582.1"
                     /translation="MKISHLGLAVSSVDGGGAIWDLLGLTEKHREEVASQKVITSFRP
                     VGESSLELLEPTSPESPIAKFLEKKGPGIHHLCLEVPDVAATLEKLKAAGIRLINETP
                     VPGAHGCLVAFIHPASTGGVLLELSQPAPGH"
     gene            complement(6810..8915)
                     /locus_tag="PLP50_13390"
     CDS             complement(6810..8915)
                     /locus_tag="PLP50_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPA52583.1"
                     /translation="MAILPARVLVLALVLLVLPFSLRGDEPAAAPAGAFRTVDDLSVP
                     DSSRPVRDLVLSFGAATVRLPEGRATVVTDPDGAPAGLFLSGKGTFTWISRDPREAPA
                     VRWTLSKNTRLSPTATEAGLAVTDSFESLLWLAPAETLPELASPATEPSLAPAFRKHR
                     DRTARVKEPPFAHLRAAQRRDAPDARVVRVETTGGKEDLAFLHDPFESGRERLTASGR
                     FQVRVSRGDDRSYAILLAEQPIRGDLFDPPSPRVVLTHVELKLTASSGSDVSISATET
                     FEARRGAVGTLLLDLVDTAESQTGATLQQTALFGDVRRGSNRIRERPVTVRGVFDEAG
                     NALEFDHRSDQLAVALREPIAEGTSARVRVEIEGGLLVRPLGNNYWFLGLAPWFPAPP
                     LSGRAFSARITMSVPKPFHPIAPGVTLRRVEDGERNVLETSLDGPAQNLAVLAGAYSL
                     EEESKDGRTLRVASYGGRNRTASRSLLDLGFATASFFEIFLGKMPVRELTVVEMAVWA
                     QGQAPAGLLLIGTEAFNPMGTGRYRRAAAGVNERFAHELSHQWWGNGVGLPDLSEQWL
                     SESFAEYCSAFFIRQAAGKEAYDLYLTRWRSRAAQTKETVPLLLAHRLRAKNDQVQAD
                     SIRTWLLYDRGPLVLAAIHREIGDEAFLTFLSSVQSTLGGKLGTTKRVEEVLEAVTRK
                     DWTPFFDRYVRGTEIPTVP"
     gene            complement(8947..9162)
                     /locus_tag="PLP50_13395"
     CDS             complement(8947..9162)
                     /locus_tag="PLP50_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52584.1"
                     /translation="MTELVWQLRKGPEGAPYAFAAFSKAEKRTNFFDGTEPLRAFLIK
                     HGAAAQDAIALVARVEGGETVSVTLPK"
     gene            complement(9176..9508)
                     /locus_tag="PLP50_13400"
     CDS             complement(9176..9508)
                     /locus_tag="PLP50_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52585.1"
                     /translation="MDPGTLVLVHLHSPKEKYWGALRALSPAGVTVRGIDVALFDDWA
                     RQFLAGGEPELGVTTLFFPLHRVEKMFEDSRVGTVAAYHERFLEMVGRDVRDVLRLDS
                     SRPDGPLQ"
     gene            complement(9574..10353)
                     /locus_tag="PLP50_13405"
     CDS             complement(9574..10353)
                     /locus_tag="PLP50_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52586.1"
                     /translation="MAIRRTLSLAGLALAGAFLLALEAPAYLIILKDGTRIEAAAKPV
                     MSGRNYIFHDKLGAQKMIAVAEVDPAKTEAANKENYRDAYILGDPEPMKRETDTSAKA
                     PSLSEYIRQNRKSQIEAPPVSAPVVPGEAASSPRGTSRSEAAAAPATVLDPIVQDSFL
                     RAYQTAAVRGTRLTPLAGGALRVQAVTDTETVVFGALVGTARGLKESRTAGRLVEKVE
                     LYMTTSAGENAGKFVILPEDAEALLNGTVPPAKFFVAHVEL"
     gene            10432..>10936
                     /locus_tag="PLP50_13410"
     CDS             10432..>10936
                     /locus_tag="PLP50_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPA52587.1"
                     /translation="MLIVMDRSATAADVGRVVEAAERMGLKAHPIPGEQRTAVGITGN
                     RGTVEPTAFENLPGVLEVIKVSHPYKLVSREFHPEDTVVSIGGVPVGGTRLAIIAGPC
                     AVETLEQTVTIAKEVKARGAHLLRGGAYKPRTSPYSFQGLGEEGLKILAAARDETGLP
                     VVTEALDT"
BASE COUNT         1504 a         3833 c         4143 g         1456 t
ORIGIN      
        1 tcatcgcccc ggcggcgttc gaggcgatgt tcgtctcgac ggcgcacacg gagaaagacc
       61 tcgcgaagac gctcgcggcc catcgcgagg cgctgaagga ggccttctcg gaaggctgag
      121 gaccgagggc ggccggggag gtccccggcc gcccgtccgc gtcgcgcgtc cttccccggg
      181 gcgtcgcgct tcggcccggc gtgcggccgc gttcggcctg gcctatcgtg ggtcggtgac
      241 gcgcccgccc tggaccccgc cggagaccat cgagacgccg cggctccgac tgcgtcgccc
      301 gcggtcggac gacgcggagg ccgtcttcgc gtacgcgagc gatcccgtcg tgacccggct
      361 gatggactgg ccgacgcacc tctcgatcga gacgagccgg cgcttcctcg cgtactgcga
      421 gacgggctgg gagacgggaa cggagttcac gtggctgctg acgctccctc cctcgggccg
      481 ggcggtcggc gcgatcgcct gccggccgaa ggagcgcggc gtggagctcg gctacgtcct
      541 ccaccgcgac gtgcacggcc gcggcctggc gaccgaggcg tcgcgcgccg tcgtcggatg
      601 gctgaaggcg gtgccggacg tcgtgcgcat cgaggcgacg tgcgacgtcg agaacgaggc
      661 ctcggcccgc gtcctcgaga aggccggcct cgcgcgcgag cggctgctgc cggcgtacgg
      721 ggcccggccg aacctcggcc ccgagccgcg cggcgcgctc ctcttcgcct ggacccgaac
      781 ggcggactga cggccgcgag gcagagaagg gagtccgagg agatgtcgct cagcgttttc
      841 gacgatccgg gccagccgcc ggggccggcg gtcctccgcc gccggctcgg ccccgcggcg
      901 actgcatgga aggcgctcgt ggacggcgtc gcggagcgct gcggtccggt cgaggagctc
      961 tggagcttcg ggggcgcgaa gttcggctgg tcgcttcggc tcctccggaa gggacgcgtc
     1021 ctcctctacc tgactccgca ggagggacgg ctcctcgccg gcctcgtcct cggcggaaag
     1081 gcggtcgcgg cggcgcggga agccggcctg ccgccggcgc tcctcgcgca ggtcgaggcg
     1141 gcgccgcagt acgccgaggg ccggggtctc cgggtcgagg tctcggggcc cgatggggtg
     1201 gaaggcctcc ttctcctcgt cagcctcaag gcggacccgt ccgtcgcgcc gtccgtcacc
     1261 gcggcgagcc cttcgcgagc cgcgcgaccg cgcggtggcg ggggcatccg acgaggtggt
     1321 cgttgacgac cccgacggcc tgcaggtagg cgtagacgat cgtcgggccg acgaaccgga
     1381 agccgcgctt ccggagctcc ttcgagatcg actccgcgag gggcgtcttc gcggggacgt
     1441 cccgcatctc ccggaacgct ccctggatcg gcctcccgcc gacgtgcgcc cagagccagg
     1501 aggcgaagct ccctgcctcc ttctgaagag cgaggtacgc cctcgcgctg tcgatcgcgg
     1561 attcgatctt gaggcggttc cggacgatgc cggcgtcgcc gaggaggcgc tccacgtccc
     1621 ccggaccgaa gcgcgccacc cttccggggt cgaagccggc gaacgccctc ctgtagccct
     1681 cgcgtttccg gaggacggtg agccaggaga gcccggcctg ggctccctcc aggacgagca
     1741 tctcgaagag gtgtctctcg tcgcgggagg ggactcccca ctcctcgtcg tgataggcga
     1801 catagagcgg gtccgagccc gcccaggggc aacgggtgcc ttcgagcatg acggggatga
     1861 tctcatcggg accggcgtgg ggtgacgggc gccgaaggca cttccgttcg atgcgcccga
     1921 tcgttcgcag cgtcccggca gaggcggtcc tccacggatc gctcccgggc cggatcttcc
     1981 ggaccctcct cgccgccctg ctggccgcct ccgccgtcct cctggcgggg tgagcggggc
     2041 aggacccgct cagcggacga gcgcctccgc gaggagcgcg ccgagccgct cgtactccgc
     2101 cttcgcgttg tagagctggg cggagacgcg gacgagcatt ccgcccggcg aaggccacgg
     2161 gtagagccac gtctcgacgc ttcgctcccg gagccacagg cccagcccct gcggtccgag
     2221 gcgcgcggcg accgagccgg gcgtggccat cgggagcggg acggacgcca tcgacccgat
     2281 catctccgcg ggggcggccg gggagacgcg gagcgtctcc ccgagcacgt cccgcgcctc
     2341 gagggcaagg gcgcggttcc tcgtcatcac ctcggaccag ccgccgggca gcagggacgc
     2401 gagcgcgtcg agcgccgccg ggatcgtcag ccagggggtc gggtcgcacg tcccggtcca
     2461 gtcgaactcg gcgcggaaac gcgctggcga cgccacctcg gagtacccga ggctcgtgac
     2521 ggcgggccgt gcgcccgtct ggaggtcctc ccggacgtgg aggaacgcgg cccccttcgg
     2581 agagcagagc cacttgtgcg cgttcccggt gtaccagccg gcgcccaggc ggtcgaggtc
     2641 gaccggcacc atccccggcg cgtgggcgcc gtcgacgaga gtctccacgc cccgctcgcg
     2701 aagcgcgccg acgaggcgct cgatcgggaa gacgagcgcc gtcgggctcg tgacgtggtc
     2761 gaggagcgcg aggcgggtcc gaggggagac ggccgcgagg acgggggcga cgacttcgtc
     2821 ctcgccctcc agagggaacg ggacgtcggc cacgacgagg cgtgccccgg tgcgggcgca
     2881 ggcgtgcgcg agcgcgttgc ggcagccggg gtaggcgtgc gtcgtcgtca gcagctcgtc
     2941 gcccgggttc aggtcgaggg agccgaggac ggcgttgacg ccggtcgtcg cgttcgggac
     3001 gaacgcgagg tcgtcgggct cggctccgag gaaccctccg aggacggcgc ggacttcggc
     3061 gaggcgctcc gggagccgac ggacgaggaa atcgaccggc tctctctcga gctcgtcgcg
     3121 aagctcggcc tgcctctcga gaacggcgac ggggcacgcg ccgaaggagc cgtggttcag
     3181 gaaggagacg tccgggtcga gtcgccagaa gcgcgcgaag gagctctctg cggccggggc
     3241 ctcgcggggc gcccggccgc ccgcctcacc cgtcacgcga ggccgtaccg ctcgaggcgg
     3301 gtgtagagct gggcacgtgt gaggccgagg agcctggccg cgcggctccg gttgttcttc
     3361 gccctctcga gcgcctgcgt gacgagggtc ttctcgaggg cgtcgaggtc gagcccggtg
     3421 ggcgggagct cgatggccgc ggcggaggcg acccgcgcgg cgctgctccg ggcgaccgcg
     3481 atggggaggt gctccacctg gacgaggccc ccctccgaga ggatgaccgc acgctcgagc
     3541 gcgttccgca gctcgcggac gtttcccggc caggagtacg cgaggaggag ctcgcgcgcc
     3601 tcgcgcgaga cgccgggggc cgggcgcccg acgttccgcg agaggtcctc gaggaagcgc
     3661 tccgtgagga ggaggacgtc ctcggggcgt tctcgaagcg gagggaggtg gatctcgaag
     3721 acggagagac ggtagaagag gtcctcgcgg aagtcgccgc gggccatcgc ggcgtggagg
     3781 tcccggttcg tcgcggcgac gattctcacg tcggcccgga gcaccttcgc gccgccgagc
     3841 cgctggaact cccgcgcctc gaggacgcga agcagcttgg cctggacgag ggggctcgtc
     3901 tcccccgcct cgtccaggaa gagggtcccc ccggcggcct gctcgagccg ccccgcgcgc
     3961 gaggcgtagg ccccggtgaa cgcccccttc tcgtacccga agagctccga ctcgaggagc
     4021 tgctcgggga gcgcggcgca gttgagcgcg acgaagggac ccgagctccg tgcggaggcg
     4081 cggtggaccg cgcgcgcgac gacctccttg ccggttcccg actcgcccgt caggaggacc
     4141 gtcgtctcgg tcggagcgac cttggaggcc tggacgagga ccgagcgcca ggcgggcgag
     4201 ccgccgacga cccgcccgaa cccgtcgcgc gagtcgagct cgtcggagag cgcgcggacg
     4261 cgggcttcga gccgggccgc ctcctgccgg gcctcctccg cccggcgggc ctcatcggcg
     4321 agccgctggt gggagaacgc gagggccacc tggtcggcga cccggagggc gaggtccacg
     4381 tcctcctcgc gaaagctcct cacctcgcgg gcgtggaagg agagcgctcc gacggtcttc
     4441 ccttcgagcc ggatcgggac cctcaggctc gaccggagcc cggccgcgag gaggatcgcc
     4501 ctgcgggagg agtcggccgg gaggtcggcc tccgcgtcgc agacgatctg gtagtcagcc
     4561 accatctgcg cgcgttccga gtccgggatc gggtaggtgt cggggaccca gtcgcggtcc
     4621 ccggagacgg cccacatccg gttctccgtc cggtcctccg acaggagccc gagcgtcatc
     4681 cggtcgtgcg ggaggacctc gcgcgcgatc gccgagacct ggtcgaagac ctcgcggacg
     4741 tcgagcgccc tcgcgagggc gggggcgagg gcgtcgagct cccggtgccg ccggacccgc
     4801 cgccgctcct cctcggcgag ccgctcgtgc tcgagcgtgg ccgagaggat gtcggcgatc
     4861 gcctgggccg tcggctcgtc ggcgggcccg aacccacctg tcgcgcgcga ggcgaaccag
     4921 aggtccccga gcgggacgtc gccgcggacg atcgtcgcga cgagcatcga gcgcattccc
     4981 gcctcgagga gctcgcggtc cccctggcgc gtcgcgtcga agagggcggc gacgtcgtcg
     5041 atcctccgcg gcgacgaggc cccgggccag agaacgtcgg agaggtcttt ccggccgtag
     5101 cggcggcccc cttcgacgtc gcccggcgtc gagaggcccc acgagaggag ggcggggcct
     5161 tcgacgagcc ggacgacgcc ggcggcgtcg aacggcagga cgcggcgcgc ggcgccggcg
     5221 acgcgggcga agacctccgg aagcgacagc gtttccgtca cggttcgggc gatctcggtc
     5281 aggacgccga cgagcggatc gaccgcgcgc gcgtccctgg ggttggttgg cacggaacgg
     5341 gcattgtacc gggcgtccct tagactctgt cggccatgat tcccgttcgc gagctcgccg
     5401 agcgcgtcct cgcggggacc ccgccggcca tcgcccgggc catcacctgg gccgagacct
     5461 cggacccgcg attcccggaa ctcctcgccc acctctggcc ccggacgggg cgtgcccggg
     5521 tcgtcggcct gacgggcgca cccggcgccg gcaagtcgac gctgacggcc gcgctcgcga
     5581 aggcatcgcg cgccgagggg cggaaggtcg ggatcgtcgc cgtcgacccc tcctcgccgt
     5641 tcacgggagg cgcgatcctc ggcgaccgga tccggatgca ggacctctac accgacccgg
     5701 gcgtgatgat ccggtcgatg gcgacgcgcg gtcacctcgg gggcctcgcc cgggccacgg
     5761 ccgacgccgt cgacgtcttc gacgccgccg ggttcgacga cgtcctcgtc gagacggtcg
     5821 gcgtcggcca ggacgaggtc gaggtcttcc gcctcgccga gacctgcgtc gtcctcctga
     5881 ccccgggaat gggcgacgac atccaggcga tcaaggcagg cctgatggag gtcgccgacg
     5941 tcttcgtggt caacaaggcc gaccgcgagg gggccgaccg gaccgtccac gaggtccttc
     6001 agatgctcga gatgggggag cacggcgcgt tcctgccccc cgtcctgaag accgtcgccc
     6061 gaacgggcga aggggtcgcc cagctccggg ccgcgctcgc gagccacaag gagttcctca
     6121 gcggaccggc cggcgcgtcg cgccgacggg accggaccgt ccgccgcatc gagtccatcg
     6181 tccgcaatcg tctcctcgag gagctccacg gtctcgccgg gagcgacgcc tccctcgcct
     6241 cctgggcggg gcgggtcgag gcgaggagtg aagacccctt ccgcgccgcc gaggccctcc
     6301 tcgagacgtt gcggaagaac ggcgctcgaa acgcgccctc cggaggaaag atgaagatct
     6361 cgcacctcgg cctcgccgtc tcgtccgtcg acggcggcgg cgcgatctgg gacctgctcg
     6421 ggctcacgga gaagcaccgg gaggaggtgg cctcccagaa ggtgatcacc tcgttccgtc
     6481 ccgtcgggga gtcgagcctc gagctcctcg agccgacctc gcccgagagc ccgatcgcga
     6541 agttcctcga gaagaaggga ccgggcatcc accacctctg cctcgaggtc cccgacgtcg
     6601 cggcgactct cgagaagctg aaggccgccg gcatccggct catcaacgag acgccggtcc
     6661 cgggggcgca cggctgcctc gtggcgttca tccacccggc ctcgaccggg ggcgtcctcc
     6721 tcgagctctc ccagccggcg cccgggcact gaccccggcc ggagcgcctc ccggacgagc
     6781 gcgcggagcg ctcgcccggg ccggcgcatt caggggaccg tcgggatctc ggtgccgcgg
     6841 acgtagcggt cgaagaacgg agtccagtcc ttccgggtga ccgcctcgag gacctcctcg
     6901 acccgcttcg tcgtgccgag ctttccgccc agtgtcgact ggacggagga gaggaacgtc
     6961 aggaacgcct cgtctccgat ctcccggtgg attgccgcca ggacgagagg tccccggtcg
     7021 tagaggagcc aggtccggat gctgtcggcc tggacctggt cgttcttcgc ccggaggcgg
     7081 tgggcgagga gaagcgggac cgtctccttc gtctgcgctg cgcgcgagcg ccagcgggtc
     7141 aggtagaggt cgtaggcctc cttgccggcc gcctgccgga tgaagaaggc ggagcagtac
     7201 tcggcgaacg actcggagag ccactgctcg gagaggtcgg ggaggccgac gccgttcccc
     7261 caccactggt gggagagctc gtgcgcaaac cgctcgttca cgcccgcggc ggcgcggcgg
     7321 tagcgccccg tccccatcgg gttgaaggcc tccgtgccga tcaggaggag acccgcgggg
     7381 gcctgcccct gggcccagac ggccatctcg acgacggtga gctcgcggac cggcatcttc
     7441 ccgaggaaga tctcgaagaa ggaggccgtc gcgaagccga ggtcgaggag gctgcgcgac
     7501 gcggtccggt tccggccgcc ataggaggcg acgcgaagcg tccggccgtc tttcgactcc
     7561 tcttcgaggg agtacgcgcc agcgaggacg gcgaggttct gcgccggccc gtcgaggctc
     7621 gtctcgagga cgttccgctc cccgtcctcc acgcggcgga gcgtgacgcc gggggcgatc
     7681 gggtggaacg gcttcggaac gctcatcgtg atccgcgccg agaaggcccg tccggacagg
     7741 ggcggtgcgg ggaaccaggg cgcgaggccg aggaaccagt agttgttccc gagcggccgg
     7801 acgaggaggc ccccctcgat ctcgacgcgg acccgggcgc tcgtcccctc ggcgatcggc
     7861 tcgcggagcg cgacggcgag ctggtcgctc cggtggtcga actcgagggc gttcccggcc
     7921 tcgtcgaaga cgcccctcac ggtcacgggt cgctcgcgga tccggttgct gccgcgccga
     7981 acgtcgccga agagcgccgt ctgctggagg gtcgcgccgg tctgcgattc ggccgtgtcg
     8041 acgaggtcga ggaggagggt cccgacggcg ccccttctgg cctcgaacgt ctccgtcgcg
     8101 gagatcgaga cgtcggagcc gctcgaggcg gtcagcttga gctcgacgtg cgtcaggacg
     8161 acccggggcg aaggcgggtc gaacaggtcg ccccggatcg gctgctcggc gaggaggatc
     8221 gcgtagctcc ggtcgtcgcc gcggctcacc cgtacctgga accgtccgct cgcggtgagc
     8281 cgctcgcggc ccgactcgaa cgggtcgtga aggaaggcga ggtcctcttt gccgccggtc
     8341 gtctcgacgc ggacgacgcg tgcgtcggga gcgtcccgcc gctgcgcggc ccggagatga
     8401 gcgaacggcg gctccttcac cctcgcggtg cggtcccggt gctttcggaa ggcgggggcg
     8461 agggacggct cggtggcggg ggaggcgagc tcggggagag tctccgcggg cgcgagccag
     8521 aggagcgact cgaacgagtc cgtgacggcc aggccggcct cggtcgcggt ggggctcagc
     8581 cgggtgttct tcgagagtgt ccaccggacg gcgggggcct cgcgcgggtc gcgcgagatc
     8641 caggtgaacg tccccttccc cgagaggaag aggcccgccg gtgcgccgtc gggatcggtg
     8701 acgacggtgg cgcgcccctc cgggagccgg accgtggccg ccccgaagga aaggacgagg
     8761 tcgcggaccg gccggctcga gtccgggacg gacaggtcgt cgaccgtccg gaaggctccc
     8821 gcgggcgcgg ccgccggctc gtccccgcgg agagagaacg ggagaacgag gaggacgagg
     8881 gcgaggacga ggacgcgcgc cgggaggatg gccatgccgg attctgccct gcgggccccc
     8941 gccggcctac ttcggcagcg tcaccgagac cgtctcgccc ccctcgacgc gcgccacgag
     9001 cgcgatcgcg tcctgggcgg cggcgccgtg cttgatcagg aacgcgcgca gcggctcggt
     9061 cccgtcgaag aagttcgtcc gcttttcggc cttgctgaag gccgcgaagg cgtagggcgc
     9121 tccttcgggg cccttccgga gctgccaaac gagctcggtc acgggtcctc cttgctcact
     9181 ggagcggccc gtcgggccgc gaggaatcga ggcggaggac gtcacggacg tcccgcccga
     9241 ccatctcgag gaagcgttcg tggtaggcgg cgaccgtccc gacgcgggag tcctcgaaca
     9301 tcttctcgac gcggtgaagc gggaagaaga gggtcgtgac gcccagctcg ggctcgcctc
     9361 cggcgaggaa ctgccgcgcc cagtcgtcga agagcgccac gtcgatcccg cggaccgtca
     9421 cgcccgccgg ggagagcgcg cgaagcgccc cccagtactt ctccttcggc gagtggaggt
     9481 ggacgagaac gagggtcccg gggtccaact cggcctcagg cgcccgccgc cggggacggg
     9541 ctcggttcgc ggaacgcgcg gggcacgccg gcctcagagc tcgacgtgag cgacgaagaa
     9601 cttcgccggc gggacggtcc cgttcagaag cgcctcggcg tcctcgggga ggatgacgaa
     9661 cttcccggcg ttctctcccg cgctcgtcgt catgtagagc tcgaccttct cgacgagccg
     9721 gccggccgtg cgcgactcct tcagcccccg ggcggtcccg acgagggcgc cgaagacgac
     9781 cgtctcggtg tcggtcacgg cctggacgcg gagcgcccct ccggcgagcg gcgtgaggcg
     9841 cgttccccgg accgcggccg tctggtacgc ccgaaggaac gaatcctgga cgatcgggtc
     9901 gaggacggtc gccggcgccg ctgcggcctc ggaccgggag gtcccgcgag gcgaggaggc
     9961 ggcctctccc gggacgacgg gtgcggagac cggaggggcc tcgatctgcg acttgcggtt
    10021 ctgccggatg tactcggaga gggacggcgc cttggcgctc gtgtccgtct ccctcttcat
    10081 cggctcggga tcgcccagga tgtaggcgtc gcggtagttc tccttgttcg cggcctcggt
    10141 cttcgcgggg tcgacctccg cgacggcgat catcttctgg gccccgagct tgtcgtggaa
    10201 gatgtagttc cgtcccgaca tgacgggctt ggccgccgcc tcgatccggg tcccgtcctt
    10261 gaggatgatg aggtaggccg gcgcctcgag cgccaggagg aacgcgcccg cgagcgcgag
    10321 acccgcgagg gagagcgtgc gccggatcgc catgggtgcc tcctcggcgg ccggagaagg
    10381 cccggctcgc cacgatgaag tataaacagc ggttccggag ggcgagacgc cgtgctgatc
    10441 gtcatggaca ggagcgccac cgcggccgac gtgggccggg tggtcgaggc cgccgagcgg
    10501 atgggcctga aggcccaccc gatcccgggg gagcagagga ccgcggtcgg gatcaccggg
    10561 aaccggggca cggtcgagcc gaccgccttc gagaacctcc ccggagtcct cgaggtcatc
    10621 aaggtctcgc acccctacaa gctcgtctcc agggagttcc accccgagga cacggtcgtc
    10681 tcgatcggcg gcgttcccgt gggcgggacg cgcctcgcga tcatcgcggg gccctgcgcc
    10741 gtggagacgc tcgagcagac ggtcacgatc gcgaaggagg tcaaggcgcg cggcgcgcat
    10801 ctcctccgcg gaggggcgta caagccccgg acctccccct actccttcca gggcctcggg
    10861 gaggaggggc tgaagatcct ggcggccgcg cgggacgaga cggggcttcc ggtcgtcacc
    10921 gaggcgctcg acacct
//
LOCUS       DAOONL010000109        10931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6773_length_10931_cov_20.859691, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000109 DAOONL010000000
VERSION     DAOONL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_10931_cov_20.859691"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLP50_13415"
     CDS             complement(<1..913)
                     /locus_tag="PLP50_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA52588.1"
                     /translation="MSGIGERRGLVHWSITRPVGTSIIALAICIVGVSMIGRLAVDLL
                     PRIVYPQIGARVSNPGVDPEVMEQTVTKLLERRLATTENAILITSESSEGDSDVDIHF
                     SYGTDVDVALRDASTKLDQARSALPEEAEPPTIGKRDPSQIPVLNFAVSSPTRDEGWL
                     KRWCEDQLSRQLLTVEGVASVDVAGGLDREIQVRLDPERLRSYRLTVSEVLTRVREEN
                     QDIAAGRLQSTSRDVVSKTKGKFTSPADIAAVRLPLPGGGDVALSDVADVRDTHLDER
                     VFARLDGKPAVQVAISKQPDANTVQVVD"
     gene            complement(910..1959)
                     /locus_tag="PLP50_13420"
     CDS             complement(910..1959)
                     /locus_tag="PLP50_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52589.1"
                     /translation="MTRPSLVVLSTVLVSLLSGCGPDSGAAAPAAPPPQVRTLRLAPE
                     DVVLTTTVVGTLEPAARVVVAAQEEGVVTDVAVREGDRVAAGDLVVKLDDRRIQAELA
                     EAEARLLDARAQARRAKALEGDGLISPSEADTIRASEAMAEARAELLRTRASFTRITA
                     PVSGVVLARRVEVGNLASARTPLVELAAGDGLLLRVPVSELEVVHLRPGDVATVRVDA
                     LPDVSLSARIARIFPAAEGTTRQVTVELSLADPPAAVRPGFLARARMVVEKRKGVLLV
                     PEAALQRGSETPHFVWALDGDTARVRPVEPGERVNGRVVVRKGLAAGDEVVVEGFVRL
                     RDGGPARRAASESGS"
     gene            2143..3717
                     /locus_tag="PLP50_13425"
     CDS             2143..3717
                     /locus_tag="PLP50_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA52590.1"
                     /translation="MSEPGATASGEAPRGTLSRVAAVVALSFSSGLPLGLVWIAIPDW
                     MRSIGVDIRVVGLFTLAQAPWTFKFLWSPLMDRFRLPFWGRRRGWIAVAQAALALSGL
                     GLAGVGDRPETPWVALALTLGVALAAATQDIAIDAYAVDVLRKEEHGVAVGLRTGMYR
                     AAMTLSGGLSIWAAASLGWGAVNLLLGLVYLPLVLVTWRSPEPEEPVEAPRTLREAVW
                     LPFLECLSRHRAVEILVFVVLYKLADALSQSLLRPFLIDMGYGEFDRGFVLGTAGVAL
                     TIAGTFLGGIACTRLGLGNALWAFGFLQVFSNIGYVLITYQPGGRAVMYGAMAFEMLT
                     SGLGMGAFGVLLLRMTEKRFSATQYALFSSLFGLPRILGGPITGLIVDAAGWRTFFWF
                     TIAAGVPGLLLLQRFAPLGTREPVFAVETAEPRRRMTGRQIARRAVVGAVVTLVVSGV
                     WMIGLVAVKAYRKDPASGFDLAAAAAAFFQPATVGGWTQLVAVLVVGALGGLGTAALL
                     TARHGEREADGATSGA"
     gene            3836..4819
                     /locus_tag="PLP50_13430"
     CDS             3836..4819
                     /locus_tag="PLP50_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPA52591.1"
                     /translation="MSSATPIHDFILANYRNFNSRATKDAMLAYWKHVEAGGKMFWAV
                     AGAMSSAQLGITMAPAIRAGLIHGLSVTGANLEESLFRLVAHRSYKDFPDYRYLTKQD
                     DTRILEDRMRRVTDTSIPEDEAFRAVEKYVVPMWKRATERGERRFWHEYFYELVTTIP
                     ADLYEGNPDECWLLAAARAGLPIVVPGHEDSTFGNIFASYVKTGECSPSIVKSGIEYM
                     AAFYDQYRTLSEGAGVGFFQIGGGIAGDFPICVVPSIKYDLQEPVKPWAYFCQISDST
                     TSYGSYSGATPNEKITWDKLTEETPMFVIESDATIVAPLITTALLELKGAR"
     gene            complement(4868..5848)
                     /locus_tag="PLP50_13435"
     CDS             complement(4868..5848)
                     /locus_tag="PLP50_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HPA52592.1"
                     /translation="MPAASLLALLDDIATVLDDVAILTKVAAKKATAVADDVAVLSKV
                     AAQKTAGVLGDDLALNAEQVTGVRAERELPVVWGVFKGSLVNKAILVPAALAISAVAP
                     WAVTPLLMVGGLYLCFEGFEKVAHKLLHRKEEGGDRHAELAEALLRKDVDLAAIEKEK
                     IKGAVRTDFVLSAEIITIALGIVANVPFGTRVLVLTAVGLAMTVGVYGLVAGIVKLDD
                     LGLYLRQKEGASRLARLRRSLGGGILAAAPWLMRLLAIVGTAAMFLVGGGILVHGLPP
                     LHHLSEGLVARGGLVGGIGPGLLGGLVGIVAGGLAVGVMAAGGRLFRSRR"
     gene            complement(5896..7320)
                     /locus_tag="PLP50_13440"
     CDS             complement(5896..7320)
                     /locus_tag="PLP50_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPA52593.1"
                     /translation="MARLRFRETPGGALRTIDLPGGELLVGREDACQVVILERSISRR
                     HARLVATEQGWCVYDEGSGNGTFVNGHRVTDALLRHGDEVRFGMVPGWFEDDPPPVAT
                     APQPPAEPPPPPPPSPPPDRPGVPTPPLPGRLAPPTPPPAPAAGRGSQRHVPPAPPRS
                     ASSPGRAGRRSALPLVLLLFALFLPLVGISAYLLWSGRAPSTGTATGSGARSSESPTP
                     EERVPLDAGAAIEAGDEEQLEALLSSGLDPNAAGDDGLTLAHRAAWAGNAGAAKLLAG
                     KGADLARKDPLGLTPAERALAEGRCAAALPLLPREAAGPGAEGRTLLHRAAEGGCAAA
                     VRELVRRGAAIDAPDPSGLTPLHLAALGGRAEALAALLEAGASPSATTPSGRTPLHLA
                     SLGGHPAATKALLAKGADPNAKDRAGRGPLHLAAAAGDGATVAALLAAKADPHLSGPD
                     GTPLDAALAAAAWDAAELLAPPGA"
     gene            complement(7323..10565)
                     /locus_tag="PLP50_13445"
     CDS             complement(7323..10565)
                     /locus_tag="PLP50_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52594.1"
                     /translation="MRGGGPARRPRPIARLARAAAAALWLVLPAAAQAPGEGAGEPVP
                     LPPEVRLLASEGAPPPASVPPYLDINDLSAATWGGAVSAAKEAMRLVAGPMSPEEEAA
                     FDETWKPLFRFPHEKLVEWLNALNPLLGQYLATRASAGEAILGFDATQLEAAAAAAAG
                     DAAAVEEAMQSAALQERLVASLVARIEETVREIEALGRPPDSDEEQRRRKKRHEDALR
                     LLDDFPLEGEWVTEAGEVIQVKLLKRLSDGRVLFWEGSKTLFEEAEAAGFDASKPGIS
                     YSDEKKRMVFVPGIWDLMNVAEPLPDGRWAVLSWILCPEVRAYCLEGDSVEITQWLVQ
                     CMAGGRIDGSRSRRTRRPLVGDPAVPPGKSWAEVLELAEKAGPGKLEQLDNSKENVAL
                     WAGVASGELPKPPETASSPPPPRPTPVPTPPARSPAEIALEKEKVAFHETNITYFESQ
                     VAAAERDVASAQDPGAREEMTRRLLYAKDALQRERDAITTIRTGEYTRTRTEADALNA
                     AIMEKQGRDQAAYWSEVHRVLDHAPRLIAMAPEGERDRLKEFFRRQVTTEAIASGDLG
                     RIRRAASAIGTSVRAGLEKDAADAEPEAIDAAQSLAYVESVKKTCDTSLLVLSGFGGM
                     GVAVTYMGVTGYMQGGPTEAFKQAATTWSMAARVASEAMDAYTRGVVENYEAYAADPG
                     KVTIDERAAGLEGAAWSAGQAAAFAALMKGGSWALGRVVGGAGKGAPPAQKPRWKDVV
                     AEARFRQDQEGGRALVRAFRDKANQLASAGKAGASRAEVGRLRQEAEQLYRSIKTDFH
                     AKVEMNALARAGQSKLVHAYNSFDRPAMQRLRQRLEQRMQADGWSDQQYRSFSNSASK
                     GKVGMDVDLGAVEPPRYVLSGGRQVPNPEHLLWQQTLTQRTPGGLTFRRSPHEFQAAG
                     QRHLHEAFREVYGRPPGQAFTTFTTSYHPEAYRDLSWLGNRWTPHAITGRVDRRWVQQ
                     AADVSGFKINNLPREHPALGRYATLQEQCRGMVKDFDTKLRPFLRDASNKEAAKHLGE
                     LRDTMERFARNEIGPIEAERRLNLLTGGEGIIGVQDRFRILLQGLAGRRK"
     gene            complement(10627..>10931)
                     /locus_tag="PLP50_13450"
     CDS             complement(10627..>10931)
                     /locus_tag="PLP50_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA52595.1"
                     /translation="GMIYPIKLPKVGVYLLGGGTYSNLKVKGTSVVQGEATEKGWSTH
                     VGAGIDLKIGEKTTLNGDVRAVFLDASSVDELFDSVQSNYKGDFWTVTLGLNYKLF"
BASE COUNT         1371 a         4025 c         3982 g         1553 t
ORIGIN      
        1 cgtcgacgac ctggaccgtg ttcgcgtcgg gctgcttgga gatcgcgacc tggaccgccg
       61 gcttgccgtc gaggcgggcg aagaccctct cgtcgaggtg ggtgtcgcgc acgtccgcca
      121 cgtcggagag ggcgacgtcg ccgccgccgg gcagcggcag gcggacggcg gcgatgtcgg
      181 ccggagaggt gaacttcccc ttcgtcttcg acacgacgtc gcggctcgtc gactggaggc
      241 gccccgccgc gatgtcctgg ttctcctcgc gaacgcgcgt caggacctcg gagacggtca
      301 gccggtagga gcggagccgc tcggggtcga gccggacctg gatctcccgg tcgagcccgc
      361 ccgcgacgtc gaccgaggcg acaccctcga ccgtgaggag ctggcgggag agctggtcct
      421 cgcaccagcg cttgagccac ccctcgtccc gggtgggcga ggagacggcg aagttcagga
      481 ccgggatctg cgacgggtcg cgcttgccga tcgtcggcgg ctcggcttcc tcggggagcg
      541 ccgagcgggc ctggtccagc ttcgtcgacg cgtcgcgcag cgcgacgtcg acgtcggtcc
      601 cgtaggagaa gtggatgtcg acgtcgctgt ccccctcgga cgactccgag gtgatcagga
      661 tcgcgttctc cgtcgtcgcg agccggcgct cgaggagctt cgtgaccgtc tgctccatca
      721 cctccgggtc gacgccgggg ttgctcaccc gggccccgat ctgggggtag acgatccgcg
      781 gcaggaggtc gaccgcgagc cggccgatca tcgagacgcc gacgatgcag atcgcgagcg
      841 cgatgatcga cgtgccgacg gggcgggtga tcgaccagtg aacgaggccc cgccgctccc
      901 cgatcccgct cacgacccgc tctccgaggc cgcgcgccgg gcggggccgc cgtcgcgcag
      961 gcgaacgaac ccttccacga cgacctcgtc acctgctgcg agccccttac ggacgacgac
     1021 ccggccgttt acccgctcgc ccggctccac gggacggacg cgcgccgtgt cgccgtcgag
     1081 ggcccagacg aagtggggcg tctccgaccc gcgctgaagc gccgcctcgg ggacgaggag
     1141 gacgcccttc cgcttctcga cgaccatccg tgcccgcgcg aggaacccgg gccggacggc
     1201 ggccggggga tcggcgagag acagctcgac cgtcacctgc cgcgtcgtcc cctcggcggc
     1261 cggaaagatc cgggcgatcc gggcgctgag ggagacgtcc ggaagggcgt cgacccgcac
     1321 cgtcgccacg tctccggggc ggaggtggac gacctccagc tcggagaccg ggacgcggag
     1381 gagcaggccg tctccagcgg ccagctcgac gagcggcgtc cgggccgagg cgaggttccc
     1441 cacctccacc cgccgcgcga ggacgactcc ggagacgggc gccgtgatcc tcgtgaacga
     1501 ggcccgggtc cggaggagct cggcccgtgc ttccgccatc gcctcggagg cccggatcgt
     1561 gtcggcctcc gacggcgaga tgaggccgtc tccctcgagg gccttcgcgc gccgcgcctg
     1621 cgcgcgcgcg tcgaggagcc gcgcctccgc ctcggccagc tcggcctgga tcctccggtc
     1681 gtccagcttc acgacgaggt cgcccgcggc aacgcggtcc ccttcgcgga cggcgacgtc
     1741 ggtgacgact ccctcctcct gggccgcgac gacgacccgc gctgccggct cgagcgttcc
     1801 gacgaccgtc gtcgtcagga cgacgtcctc cggggcgagg cgcagcgtgc gcacctgggg
     1861 cggcggagcg gcgggcgcgg ccgcgccgga gtccggtccg cagccggaga ggagggacac
     1921 gaggacggtc gacaggacga cgagggaggg acgggtcatc gggcctccgc gccgcccatc
     1981 accgcccccg ttgcgggcgc ggcgcgggcg cggcgtccgg acaggctagc agggcggttc
     2041 gggggtgtcg aagtcgatga ggcacccctg agtaacggcg cctggcgtcg ccggtattcc
     2101 cgacgcgtcc cggcctcgcc ccgaccgtac aatcgaccgt ccatgagcga gccgggcgcg
     2161 acggcgtcgg gagaggcccc gagggggacg ctgtcgcggg tcgcggcggt cgtcgcgctg
     2221 tcgttctcct ccgggctgcc tctcggcctc gtctggatcg cgatacccga ctggatgcgc
     2281 tcgatcgggg tcgacatccg cgtcgtcggc ctcttcacgc tcgcgcaggc cccctggacg
     2341 ttcaagttcc tctggtcgcc tctcatggac cgtttccggc tccccttctg gggccggcgg
     2401 cggggctgga tcgcggtcgc tcaggccgcc ctcgccctct cgggcctcgg gctcgccggc
     2461 gtcggggacc gccccgagac gccctgggtc gcgctcgccc tgacgctcgg cgtcgcgctc
     2521 gccgccgcga cgcaggacat cgcgatcgac gcctatgcgg tcgacgtcct ccggaaggag
     2581 gagcacggcg tcgccgtcgg cctcaggacc ggcatgtacc gggcggccat gacgctctcc
     2641 ggcggcctct cgatctgggc ggcggcgagc ctcggatggg gagcggtcaa cctcctcctc
     2701 ggcctcgtct acctgccgct cgtcctcgtc acctggaggt cgcccgagcc ggaggagccc
     2761 gtcgaggcgc cgcggaccct ccgcgaagcg gtctggctgc cgttcctcga gtgcctctcc
     2821 cgccaccgcg ccgtcgagat cctcgtcttc gtcgtcctct acaagctcgc ggacgccctg
     2881 tcgcagtcgc tcctccgccc gttcctcatc gacatggggt acggcgagtt cgaccgcggt
     2941 ttcgtcctcg ggacggccgg cgtcgccctg acgatcgcgg gcaccttcct cggcggcatc
     3001 gcctgcaccc gtctggggct cgggaacgcc ctctgggcct tcggcttcct gcaggtcttc
     3061 tcgaacatcg gctacgtcct gatcacctac cagccgggcg ggcgcgccgt catgtacggc
     3121 gcgatggcgt tcgagatgct cacgagcggc ctcggcatgg gcgccttcgg cgtgctgctc
     3181 ctccggatga cggagaagag gttctcggcc acgcagtacg ccctcttctc gtcgctcttc
     3241 ggtctccccc gcatcctcgg cgggccgatc accggcctca tcgtcgacgc cgccgggtgg
     3301 cggacgttct tctggttcac gatcgccgcg ggcgtcccgg gcctcctgct gctccagcgc
     3361 ttcgcgccgc tcgggacgcg cgagccggtc ttcgccgtcg agacggccga gccgcggcgc
     3421 cggatgacgg gtcgtcagat cgcccgtcgt gcggtcgtcg gagccgtcgt cacgctcgtc
     3481 gtgagcggtg tctggatgat cggcctcgtc gcggtcaagg cgtatcgcaa ggatccggcc
     3541 tccggattcg acctcgccgc cgccgccgcg gccttcttcc agccggccac ggtcggaggg
     3601 tggacacagc tcgtcgccgt cctcgtcgtc ggggcgctcg gcggcctcgg cactgccgcg
     3661 ctcctgacgg cgcgccacgg ggagcgggag gcggacgggg cgacgagcgg ggcctgacct
     3721 cccgccggtc ccgcggcgcc gctcccgggg gaggcctccc gccctcgggc gggacgttga
     3781 gccctcccgg aagtcgggct acgctcaccg cccggaaccg aacgagggag agccgatgag
     3841 cagcgccaca ccgatccacg acttcatcct cgcgaactac cgcaacttca actcccgcgc
     3901 cacgaaggac gcgatgctcg cctactggaa gcacgtggag gcgggcggga agatgttctg
     3961 ggccgtggcc ggcgccatgt cgtcggcgca gctcggcatc acgatggccc ccgcgatccg
     4021 cgccgggctg atccacgggc tctcggtcac cggcgcgaac ctcgaggagt cgctcttccg
     4081 tctcgtggcg caccggagct acaaggactt ccccgactac cggtacctga cgaagcagga
     4141 cgacacgcgg atcctcgagg atcggatgcg gcgggtcacc gacacgagca tccccgagga
     4201 cgaggccttc cgcgccgtgg agaagtacgt cgtcccgatg tggaagaggg ccaccgagcg
     4261 cggcgagcgc cggttctggc acgagtattt ctacgagctc gtcacgacga tccccgccga
     4321 cctctacgaa gggaaccccg acgagtgctg gctcctggcc gccgcgcggg ccggcctgcc
     4381 gatcgtcgtc cccggccacg aggactccac gttcgggaac atcttcgcct cgtacgtgaa
     4441 gacgggcgag tgctcgccga gcatcgtgaa gtcggggatc gagtacatgg ccgccttcta
     4501 cgaccagtac cggacgctct cggaaggggc cggcgtcggg ttcttccaga tcggcggcgg
     4561 catcgcgggc gacttcccga tctgcgtcgt gccgtcgatc aagtacgacc tgcaggagcc
     4621 ggtgaagccc tgggcctact tctgccagat ctccgactcg acgacctcgt acggctccta
     4681 ctcgggcgcg accccgaacg agaagatcac ctgggacaag ctgacggaag agacgccgat
     4741 gttcgtcatc gagtccgacg cgacgatcgt cgcgccgctc atcacgacgg cgctcctgga
     4801 gctgaagggg gcgcggtagc ccgggccggg gcggagagcg cggaagaccg gccgcgccct
     4861 tcgccgctca gcgcctgctg cggaagagcc gcccccccgc ggccatgacc ccgaccgcga
     4921 ggcctccggc gacgatcccg acgagcccgc cgaggagccc cgggccgatc ccgcccacga
     4981 ggccgccgcg ggcgacgagc ccctcggaga ggtggtggag cggcgggagg ccgtggacga
     5041 ggatgccacc gccgacgagg aacatcgccg cggtgccgac gatcgcgagg agccgcatga
     5101 gccacggcgc ggcggcgagg atcccccccc cgagcgagcg ccggagccgc gcgaggcgcg
     5161 aggccccttc cttctgccgg aggtagaggc cgaggtcgtc cagcttcacg atgccggcga
     5221 cgaggccgta gacgccgacg gtcatggcga ggccgacggc cgtcaggacg aggacgcgcg
     5281 tgccgaacgg gacgttcgcg acgattccga gcgcgatcgt gatgatctcg gccgacagga
     5341 cgaagtccgt gcggaccgcc cccttgatct tctccttttc gatcgccgcg agatcgacgt
     5401 cctttcggag gagcgcctcc gccagctcgg cgtggcggtc gcccccctcc tccttccggt
     5461 ggaggagctt gtgcgcgacc ttctcgaacc cctcgaagca gaggtagagc ccgccgacca
     5521 tcaggagcgg cgtcaccgcc cagggcgcga cggcgctgat cgcgagcgcc gcggggacga
     5581 ggatcgcctt gttgacgagc gagcccttga agacgcccca gacgacgggg agctcgcgct
     5641 ccgcgcggac gccggtcacc tgctcggcgt tcaaggcgag gtcgtcgccg aggacccccg
     5701 ccgtcttctg cgccgcgacc ttcgagagga cggcgacgtc gtcggcgacg gcggtcgcct
     5761 tcttcgcggc gaccttcgtc aggatcgcga cgtcgtcgag gacggtcgcg atgtcgtcga
     5821 gcagggcgag caggctggcg gcgggcatcg gttcggtggg cggatgctag cagcagcgcg
     5881 ccgctccggg ggcgctcagg cgccgggcgg cgcgaggagc tccgccgcgt cccacgcggc
     5941 cgccgcgagc gcggcgtcga gcggcgtgcc gtcggggccg gagaggtgcg ggtcggcctt
     6001 cgccgcgagg agggccgcga ccgtcgcgcc gtcccccgcg gcggccgcga ggtggagcgg
     6061 cccccgcccc gcccggtcct tcgcgttcgg atcggccccc ttcgcgagga gcgccttcgt
     6121 cgccgcgggg tgaccgccga gcgaggcgag gtgcagcggc gtccgcccgg acggcgtcgt
     6181 cgccgacggc gaggcgcccg cctcgaggag cgccgcgagc gcctccgccc ggccgccgag
     6241 cgccgcgagg tgcagcggcg tcagcccgga cggatcgggc gcgtcgatgg ccgccccgcg
     6301 ccggacgagc tccctcacgg ctgcggcgca gcccccctcg gcggcgcggt gaaggagggt
     6361 ccggccctcc gcgcccggcc cggccgcttc gcgcggcagg agcggcaggg ccgccgcgca
     6421 tcgcccctcg gcgagcgcgc gctcggccgg cgtgagcccg agcgggtcct tccgcgcgag
     6481 gtccgccccc ttgccggcga ggagcttcgc cgcgccggcg tttccggccc aggcggcgcg
     6541 gtgggcgagc gtgagcccgt cgtcaccggc ggcgttcggg tcgaggccgg aggagaggag
     6601 ggcctcgagc tgctcctcgt ctcccgcctc gatcgccgca ccagcgtcga ggggaacacg
     6661 ttcctccggc gtggggctct ccgacgaccg cgcgccggag ccggtggccg tgcccgtcga
     6721 aggagcccgg cccgaccaga ggaggtaggc cgagatcccg acgagcggca ggaagagcgc
     6781 gaaaaggagg aggacgaggg gaagggcgct ccggcgaccc gcgcgaccgg gtgaagacgc
     6841 ggagcggggc ggcgccggag gcacgtgccg ttgcgagcct cgcccggcgg cgggcgcggg
     6901 aggaggcgtg gggggcgcga ggcggcccgg cagaggaggc gttggcaccc cggggcggtc
     6961 cggcggggga gacggaggag gtggaggagg aggctcggcg ggaggctgcg gcgcggtcgc
     7021 cacgggcggc gggtcgtcct cgaaccagcc cgggaccatc ccgaagcgga cctcgtcgcc
     7081 gtgccggagg agcgcatctg tcacccggtg gccgttcacg aaggtcccgt tcccgctccc
     7141 ctcgtcgtac acgcaccagc cctgctccgt ggcgacgagg cgcgcgtgcc ggcgggagat
     7201 ggagcgctcg aggatcacga cctggcaggc gtcctcgcgg ccgacgagga gttcgccccc
     7261 ggggaggtcg atcgtccgca gcgccccgcc aggagtctcg cggaatcgca ggcgcgccac
     7321 tgctacttcc tcctcccggc gagcccctgg aggagtatcc ggaagcggtc ctgcacgccg
     7381 atgatcccct cgcccccggt caggaggttc aggcggcgct cggcctcgat cgggccgatc
     7441 tcgttccggg cgaaccgctc catcgtgtcg cgcagctcgc cgaggtgctt cgccgcctcc
     7501 ttgttcgagg cgtcgcggag gaacggcctc agcttcgtgt cgaagtcctt gaccatcccg
     7561 cggcactgct cctggagcgt cgcgtagcga ccgagcgccg gatgctcgcg cgggaggttg
     7621 ttgatcttga agccggacac gtccgccgcc tgctggaccc agcggcggtc gacgcgcccc
     7681 gtaatggcgt gcggcgtcca gcggtttccg agccaggaga ggtcgcggta ggcctccggg
     7741 tggtagctcg tcgtgaaggt cgtgaaggcc tgccccggcg ggcggccgta gacctcccgg
     7801 aacgcctcgt gcaggtgccg ctgccccgcc gcctggaact cgtgcggcga gcgccggaac
     7861 gtcagcccgc ccggcgtccg ctgcgtgagc gtctgctgcc agaggaggtg ctcggggttc
     7921 gggacctgcc ggccgcccga gaggacgtac cgcggcggct cgacggcgcc gaggtcgacg
     7981 tccatcccga ccttcccctt gctcgccgag ttgctgaagc ttcggtactg ctggtcgctc
     8041 cagccgtcgg cctgcatccg ctgctcgagc ctctgccgga ggcgctgcat cgcggggcgg
     8101 tcgaaggagt tgtaggcgtg gacgagcttc gactgccccg ccctcgcgag ggcgttcatc
     8161 tcgaccttcg cgtggaagtc ggtcttgatc gaccggtaga gctgctcggc ctcctggcgg
     8221 agccgcccga cctccgcgcg cgacgccccc gccttcccgg ccgaggcgag ctggttcgcc
     8281 ttgtcgcgga aggcgcggac gagggcgcgc cccccttcct ggtcctggcg gaagcgcgcc
     8341 tcggcgacga cgtccttcca tcgcggcttc tgcgcgggcg gcgccccctt ccccgcgccg
     8401 ccgacgaccc gcccgagggc ccacgagccc cccttcatga gcgccgcgaa agcggccgcc
     8461 tgcccggcgc tccaggcggc cccttcgagc cccgcggccc gctcgtcgat cgtcactttc
     8521 cccgggtcgg cggcgtacgc ctcgtagttt tcgacgacgc ctcgcgtgta ggcgtccatc
     8581 gcctcgctcg cgacgcgcgc cgccatcgac caggtcgtcg ccgcctgctt gaaggcctcc
     8641 gtcgggccgc cctgcatgta gccggtgacg cccatgtagg tgaccgcgac ccccattccg
     8701 ccgaagccgg agaggacgag gagcgacgtg tcgcacgtct tcttcacgct ctcgacgtag
     8761 gcgaggctct gcgcggcgtc gatcgcctcg ggctcggcgt cggcggcgtc cttctcgagg
     8821 ccggcgcgca cggaggtccc gatcgcgctc gcggcgcggc ggatccggcc gaggtcgccc
     8881 gaggcgatcg cctccgtggt cacctggcgg cggaagaact ccttcagccg gtcccgctcc
     8941 ccctccggcg ccatcgcgat gaggcgcggc gcgtggtcga ggacgcggtg gacctcgctc
     9001 cagtaggccg cctggtcgcg cccctgcttc tccatgatcg cggcgttcag ggcgtccgcc
     9061 tcggtccggg tccgggtgta ctcgccggtc cggatcgtcg tgatcgcgtc cctctccctc
     9121 tggagcgcgt ccttcgcgta gaggaggcgc cgcgtcatct cctcgcgcgc gccggggtcc
     9181 tgcgccgagg cgacgtcccg ctcggcggcc gcgacctgcg actcgaagta ggtgatgttc
     9241 gtctcgtgga aggcgacctt ttccttctcg agcgcgatct ccgcgggact cctcgcgggg
     9301 ggcgtcggaa cgggcgtcgg gcgtggcggg gggggagacg acgcggtctc cggcggcttc
     9361 gggagctcgc ccgaggcgac ccccgcccag agcgccacgt tctccttcga gttgtcgagc
     9421 tgctcgagct tgccggggcc cgccttctcg gcgagctcga ggacctcggc ccagctcttc
     9481 cccggcggga cggccgggtc cccgacgagc ggccggcgcg tccggcggga ccggctcccg
     9541 tcgatccgcc cgcccgccat gcactggacg agccactgcg tgatctccac ggagtcccct
     9601 tcgaggcaat aggccctcac ctccgggcag aggatccagg agaggaccgc ccaccgtccg
     9661 tccggcagcg gctccgcgac gttcatcagg tcccagatcc cggggacgaa gaccatccgc
     9721 ttcttctcgt ccgagtacga gatccccggc ttgctcgcgt cgaagcccgc cgcctcggcc
     9781 tcctcgaaga gggtcttcga gccctcccag aagaggaccc gtccgtcgga gaggcgcttc
     9841 aggagcttga cctgtatcac ctcgccggcc tccgtgaccc actccccctc gagggggaag
     9901 tcgtcgagga gccggagggc gtcctcgtgg cgcttcttcc tccgccgctg ctcctcgtcg
     9961 gagtcgggcg gccggccgag cgcctcgatc tcgcggaccg tctcctcgat ccgggccacg
    10021 agcgaggcga cgaggcgctc ctggagcgcg gccgactgca tcgcctcctc gacggcggcc
    10081 gcgtcgccgg ccgccgccgc ggcggccgcc tcgagctgcg tcgcgtcgaa gccaaggatc
    10141 gcctctcccg ccgaggcgcg cgtcgcgagg tactggccga ggagcgggtt cagggcgttc
    10201 agccactcga cgagcttctc gtgcgggaac cggaagagcg gcttccaggt ctcgtcgaag
    10261 gccgcctctt cctccgggct catcggcccg gcgacgagcc gcatcgcctc cttcgccgcg
    10321 gagaccgcgc cgccccacgt cgccgcggag aggtcgttga tgtcgaggta gggcgggacg
    10381 ctcgcgggcg gtggcgcgcc ctccgacgcg aggagccgga cctccggggg gagcgggacc
    10441 ggctcgccgg ccccctcgcc cggcgcctgg gccgcggcgg ggagcacgag ccacagggcg
    10501 gcggccgcgg cgcgcgcgag ccgcgcgatc ggtcgggggc ggcgggccgg cccacctccg
    10561 cgcatgccgc ggacagcgta gcaccgcccg cgggccggag acgcccccgg cccgcgggct
    10621 ctcgggtcag aagagcttgt agttcaggcc gagggtgacc gtccagaagt cgcccttgta
    10681 gttcgactgc acgctgtcga agagctcgtc gacgctgctg gcgtcgagga agacggccct
    10741 cacgtccccg ttcagcgtcg tcttctcgcc gatcttcagg tcgatccccg cgccgacgtg
    10801 ggtcgaccac cccttctcgg tcgcctcgcc ctggacgacg ctcgtcccct tcaccttcag
    10861 gttgctgtag gtgccgccgc cgaggaggta gacgccgacc ttcgggagct tgatcgggta
    10921 gatcatcccg g
//
LOCUS       DAOONL010000110        10924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6784_length_10924_cov_11.909283, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000110 DAOONL010000000
VERSION     DAOONL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6784_length_10924_cov_11.909283"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..3444
                     /locus_tag="PLP50_13455"
     CDS             364..3444
                     /locus_tag="PLP50_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA52596.1"
                     /translation="MIRELGKGAMGVVYLGRDPVIGRLVALKTIRAVSEDDVEQKEFQ
                     ERFLREAQAAGILSHPNIVTVHDVGEDAATSTSFIAMEYVEGKNLKQLVTEKTTFGFE
                     RIAEIIGQVAEALDYAHRRGIVHRDVKPANIIITPEGQVKITDFGIAKIEKSNLTTTG
                     QFLGTPNYMSPEQVTGDAVDGRSDLFSLGVVLYELLTRKKPFTAENLTSISYKIVHER
                     FVPPETYDAGIPSEFAGVLDRALAKDPAERFQRGNDFALALFEFKAREEERQMLRDLG
                     QMVAEAEKLGPVATVTAPPTTLSGPATAMPEAPAAARSPFDILAMAPAAAAAPVPPAP
                     DDDLATREVVRPLPATAYVAGLAPSSPGTDSESRSGGSEEWQLDEPTRSKKKSAPPPP
                     PPVAGDVDVEASLPGAEVVEAAPAVPVAPLPPSAPPPAVAGLAVSPFDEAPPERRPEG
                     ATEILRIDAIPGLTAAPAGDRPTEILKPIAPPGPMAPAPPPAVPPPVADRPTEILRVV
                     PPPSRVEDPPTPTDQPTEILRAVAPPPVSAPAVPAAAPPAPAAPSPAPFVDRPTEILK
                     PIAPPAPAAPPPAPFGASIPAPGGSSAPVGSDRPTEILRTPFPPPGPASAARPPVPRP
                     TGDAAGEEGATERIVDAMKLARAGGPAAPVSPMAPPPTVAAPKGAPAPASPPKGVPAP
                     ATPPRGVPAPPPAALPPVELAPIPEPHAALPAPPTGPVMKRPLNLRFVALIIGLVAIG
                     GAVVTGVLWSQKKKVEAQAAVNEIREREVQEEKALMVEGTRLLDAGQPTAALEKFREL
                     IRRKPDSEAVRAAIAKAEELARAQQSGEALSREIETLMATAREATELGEFDRALDQLA
                     QVLLLQPENAEALALRDSVNTAQAEQTAAQKKAAAAEAARRRRPTPVPTPVPVARATV
                     PAEVPRAPTPTPGVARLRIIFQSPAPEGYVMIRRDDKEVFRRNFDFGRKSAGGLVEGT
                     IEVPAGSASFKAWAIPTNRSFNGYEAVDLVIPGGETRTLLLELNAEKKLVVRLR"
     gene            3410..5152
                     /gene="ggt"
                     /locus_tag="PLP50_13460"
     CDS             3410..5152
                     /gene="ggt"
                     /locus_tag="PLP50_13460"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPA52597.1"
                     /translation="MQRRSSSYGFARHLLFALATLPLLAAAARGGGGGGAGSVEARPT
                     AQVLTSNRGMVVAAAPEASRAGAEVLAAGGNAMDAALAAALALAVVYPQAGNLAGGGF
                     LVVRTPDGVVRALDFRETAPAGASSRMFLGKDGRPVPGASTSTALAVATPASVRGYAE
                     AHRRLGRLPWAAVVAPAERLAREGFVIPDGLSHDLEDERGLLTRWDETRRVFFPGGRP
                     LAPGTRLKQPELAETLARIGREGADAFHRGEIAARIAAFVREAGGILSEGDLAGYAPV
                     WRAPEEIRFGDLVVHTMPLPSSAGLVLRSVLAQLEVARGPGAFRPDAAAYHVLLEAER
                     RAYADRNRWLGDGDCVEVPLPELLEPERLVRLGASIDHERATPSGSVPGGLAREREET
                     THLSVATPDGFAVSLTTTLNGSFGNGAIVPGTGVLLNNQMDDFAAAPGTPNMYGLVQG
                     SANAVRAGARPLSSMAPAVVEKAGRPFLVVGSPGGSTIPTTVLQVLLRTAGGEDLGSA
                     VAAPRLHHQHTPDVVFVERGRAASEVLEGLRRRGHALRERGPIGKVHAVSFSPDGRLV
                     GAADPRGYGASAAP"
     gene            5245..5321
                     /locus_tag="PLP50_13465"
     tRNA            5245..5321
                     /locus_tag="PLP50_13465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5279..5281,aa:Pro,seq:cgg)
     gene            5416..6183
                     /locus_tag="PLP50_13470"
     CDS             5416..6183
                     /locus_tag="PLP50_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPA52598.1"
                     /translation="MGNGGVSVTVDGRSATVTFWSPPENVLDRETVVALESRLREVAR
                     RRDVRLVALAARGEDFCNGAEVVPPVDGRPQALLEAWHSLVRFLLTCEVPTCALVQGR
                     ALGAGAELALACDFIFAETTASFGFPEIHRGSFPPVAAVLLERRAGRTKAADLILCGA
                     AMTAEAAERRGLINALVNPGDLIEALETMKTRLETLSASSLRLAKRALNQGCSGDLLS
                     ALSAVERTFSRELLKTEDAAEGARAALEGRPPVWTDR"
     gene            complement(6338..7180)
                     /locus_tag="PLP50_13475"
     CDS             complement(6338..7180)
                     /locus_tag="PLP50_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52599.1"
                     /translation="MSREGAVDRGRIRGYLLRRLGDDERQTFEESYFADDALLDRVEA
                     EEDLLVTDYVLGRLSDEDRRRFEQALLGTPYYRERVETTTRLRRRIARDHKVRSLGRG
                     APPRSGGGLFPGRTGVVVAFGLLCVLLLAALVTALRLKAQLATLRAELSTVPPAVVTA
                     AGGGVVPSTPSVVLQPGEGVGVSVRRVERLAGSPLLLVFPRHLLSGEARSWRVVLREE
                     SGAVTWDSGDQAVPSGASADMSVRLPAGVPAEGRSGVTLWDGGTPTFSTLLEVAPQPS
                     APPR"
     gene            complement(7177..7761)
                     /locus_tag="PLP50_13480"
     CDS             complement(7177..7761)
                     /locus_tag="PLP50_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA52600.1"
                     /translation="MTTSWADPFEAELGRLPEKLRRFFLSRGVRTEDEARDLVQETLL
                     RVLQRASRGEPLESPEAFALGVAKNVFFEYCRRVQKANRHEGLSGVLELAGTEDPLSD
                     ALSSSRRLIVRKALELLPARMRELLVRRFVEEVPSRTIAREEGITTDAVDMRVYRAKR
                     ELRKKMDSTAARSAVRAQREEEARRGPGEDGGEA"
     gene            8078..8692
                     /gene="lexA"
                     /locus_tag="PLP50_13485"
     CDS             8078..8692
                     /gene="lexA"
                     /locus_tag="PLP50_13485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPA52601.1"
                     /translation="MANRPNLTERQLEIVAFIRDYRARRGIAPTQREICEHFGYSSFG
                     TLQKHIRLLLDKGVLVRDWNQRRSLDVAEEAASAPALEVPLLGRIAAGSPIEVLPGDD
                     RLHVPESLTRKGENFVLLVSGDSMIDEGIRDGDWVVVHRRTRAENGEMVAALVGGEAT
                     LKRLYREAEGIVRLQPSNERLAPIRAREEDVQVQGIVVGLMRRY"
     gene            8893..9486
                     /locus_tag="PLP50_13490"
     CDS             8893..9486
                     /locus_tag="PLP50_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52602.1"
                     /translation="MDRRDEEPIEASERRSRGAILRLAGAGFASILAIAVPLDGSAGV
                     SAGGPVAPAPDDAPAPTTLPPGWPGETPDLATPRQLDVWSIVHAACRRHGIEDEAVTM
                     YHVLWEESTLRGHVSSPCGRYHGIGQFTLSTFRESVGKMRRLRLIWGDEEWSPFDPVQ
                     AIEVMAFMWSRGYHDHWGPYRRVVRRQARALAAASLN"
     gene            complement(9539..10411)
                     /locus_tag="PLP50_13495"
     CDS             complement(9539..10411)
                     /locus_tag="PLP50_13495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA52603.1"
                     /translation="MNVLPRVLGPGDEALVRAFLGAHADSSLFLLGNLRAAGLADTGA
                     AYSATWVAAFDGPYVAGVAAHCWNGVLLLQAPRGLAQLARAALSASGRPLAGLSGPRG
                     QVVAARAALGLDGAPAALDHEAELFSLALDGLVVPEGLSTGDVACAPPSPEDRSLLVD
                     WRVAYAVETLGAVPGEGLPAACAEEVDRLVVEGNAFVLRRAGVPVAFSGFNARLPEVV
                     QVGGVFTPSELRGRGHARAVVAGSLLAARAAGVSRAVLFTERENEPARRAYLALGFRV
                     TGDYGMVLFGSTGA"
     gene            complement(10408..>10924)
                     /locus_tag="PLP50_13500"
     CDS             complement(10408..>10924)
                     /locus_tag="PLP50_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPA52604.1"
                     /translation="LGVDQPLRVGDFVKVGDVLGTVEKIGLRSTRFRTLDRTLVTIPN
                     GKLADMQIETFAERDRIRLATILNLEYGTTAAQMREVVSGIEAMLRAHPKIWPDTVVV
                     RFVAFAGSSLDVEVMAWFQTTSFDEFRDIRQEALIGILEIVEKAGTSFAFPTRTVHLV
                     GPAATLPAPRP"
BASE COUNT         1588 a         3892 c         3922 g         1522 t
ORIGIN      
        1 ccgtcgtcga cgcggccgaa gcggttcacc gctcccgcct cgtgaaggag cgccgagacg
       61 agggtggtct tcccgacgcc gttgtggccg gcgacggcca cgttgcgaat ggcggttgcg
      121 ggatactgtt tcatgcttcg tcccccttgg cgaggtctct cgaaaggcct gcattatagg
      181 gatcgacgct gcttcgccga agcggcgttt cgtgcgttga caccctcttt cacgcggccc
      241 taaactgcgc cacaccggga aagtcccggc atcctattgc cgccgtcgcg tacgggagga
      301 tctctgccga acgtatccgg tgcgttctcc tcgggggctc cgaagaggtt cggccgctac
      361 gaggtgattc gcgagctggg gaagggcgcg atgggcgtcg tctacctcgg ccgcgacccc
      421 gtcatcggcc gactcgtcgc gctgaagacc atccgggccg tcagcgagga cgatgtcgag
      481 cagaaggagt tccaggagag gttccttcgc gaagcccagg ctgcgggcat cctttcccac
      541 ccgaacatcg tcacggtcca cgacgtcgga gaggacgccg cgacgtccac gtcgttcatc
      601 gcgatggagt acgtggaagg gaagaacctc aagcagctcg tcaccgaaaa gacgacgttc
      661 gggttcgagc ggatcgcgga gatcatcggg caggtggccg aggcgctcga ttacgcccac
      721 cgccgcggga tcgtccaccg cgacgtgaag ccggcgaaca tcatcatcac ccccgaaggg
      781 caggtgaaga tcaccgactt cgggatcgcg aagatcgaga agtcgaacct caccacgacg
      841 gggcagttcc tcggcactcc gaactacatg tcgcccgagc aggtgacggg cgacgccgtc
      901 gacggccgga gcgacctctt ctccctgggc gtcgtcctct acgagctgct gacgcggaag
      961 aagccgttca cggcggagaa cctgacgtcg atctcgtaca agatcgtcca cgagcggttc
     1021 gtcccgcccg agacctacga cgccgggatc ccgtccgagt tcgcgggggt tctcgacagg
     1081 gcgctcgcga aggacccggc agagcggttc cagcgcggga acgacttcgc cctcgccctg
     1141 ttcgagttca aggcccggga ggaagagcgg cagatgctcc gcgacctcgg gcagatggtc
     1201 gcggaggccg agaagctcgg cccggtcgcc acggttacgg ctccgccgac gaccctgtcc
     1261 ggtccggcta cggcgatgcc cgaagctccg gcggccgcac ggtcgccgtt cgacatcctc
     1321 gcgatggccc ccgcggccgc cgcggctccc gttccgccgg cgccggacga cgacctcgcc
     1381 acgcgcgagg tcgtccggcc gctccccgcg acggcctacg tggccggact ggctccttcg
     1441 agccccggta ccgactcgga gtcgcgctcg ggcggttccg aggagtggca gctcgacgag
     1501 ccgacccgct cgaagaagaa gagcgcgcct cctccgcccc cgccggtcgc cggcgacgtc
     1561 gacgtcgagg cgtcgctccc cggcgcagag gtcgtcgagg cggccccggc cgtcccggtg
     1621 gccccacttc cgcccagcgc tccgccgcca gcggtcgccg gcctggcggt ctccccgttc
     1681 gacgaagcgc cgcccgagcg gcggcccgaa ggagcgacgg agatcctccg gatcgacgcg
     1741 atcccgggcc tcaccgcggc gccggccggc gaccggccca cggagatcct caagccgatc
     1801 gccccgccgg gtccgatggc gcctgcaccg cccccggccg ttcctccgcc ggttgcggat
     1861 cgcccgaccg agatcctgcg cgtcgtcccg cctccctccc gtgtcgagga cccgccgacg
     1921 ccgaccgatc aacccaccga gatcctcagg gcggtcgctc cgccgccggt ttccgcgcct
     1981 gccgttcccg ctgcggcgcc tccggccccc gcggctccgt cgcccgcgcc cttcgtcgat
     2041 cggccgaccg agatcctcaa gccgatcgct ccgccggctc ccgcggctcc gccacccgcg
     2101 cctttcggtg cctcgatccc ggcgccgggc gggtcgtccg cgcccgtcgg ctcggatcgg
     2161 ccgacggaga tcctccgcac cccgttcccg cccccgggcc ccgcctctgc cgcgcggccc
     2221 ccggtcccgc ggccaaccgg agacgcggcc ggggaggaag gcgcgacgga gcgaatcgtc
     2281 gacgcgatga agctcgcgcg agccggggga cctgcggcgc ccgtttcgcc gatggccccg
     2341 cctccgaccg tcgccgctcc gaagggcgcg cctgcaccgg cttcccctcc gaagggcgtc
     2401 ccggctccgg caaccccgcc gaggggcgtc ccggctccgc cgccggccgc gctcccgccc
     2461 gtggagctcg cgccgatccc ggagcctcac gcggctctgc ccgcgccgcc gacgggaccc
     2521 gtgatgaagc gccccctgaa cctgcggttc gttgcgctca tcatcggcct cgtcgccatc
     2581 ggtggcgccg tcgtgaccgg cgtcctctgg tcgcagaaga agaaggtcga ggcccaggcg
     2641 gccgtcaacg agatacgaga gagggaagtc caggaggaga aggcgctgat ggtcgagggg
     2701 acccggctcc tcgacgcagg tcagccgacg gcggccctgg agaagttccg cgagctcatc
     2761 cgccgcaagc cggattccga ggcggtgcgg gcggccatcg cgaaggccga ggagctggcc
     2821 cgggcgcaac agtccgggga ggcgctctcg cgcgagatcg agaccctcat ggccacggca
     2881 cgcgaggcca ccgagctcgg ggagttcgat cgcgcgctcg atcagctggc gcaggtcctg
     2941 ctgctccagc cggagaacgc cgaagcgctc gcgctccgcg attccgtcaa caccgcgcag
     3001 gccgagcaga ccgccgcgca gaagaaggcg gcggcagccg aggccgcccg ccggaggcgg
     3061 cccacccccg tcccgacgcc cgtcccggtc gcgcgggcta cggttcccgc cgaggtgccc
     3121 agggcgccga cgccgacgcc gggcgtcgcg cggctccgca tcatcttcca gagcccggcc
     3181 cccgagggct acgtcatgat ccggcgggac gacaaggaag tcttccgcag gaacttcgac
     3241 ttcgggcgca agagcgccgg ggggctggtc gaggggacga tcgaggtccc ggcgggaagc
     3301 gcgagcttca aggcctgggc catcccgacg aaccggtcgt tcaacggcta cgaagcggtc
     3361 gatctcgtca tcccgggcgg cgagacgcgc acgctcctcc tcgagctgaa tgcagagaag
     3421 aagctcgtcg tacggcttcg ctaggcatct cctcttcgcg ctcgcgaccc tcccgctcct
     3481 cgccgcggcc gcccgcggcg ggggaggggg gggcgccgga tcggtcgagg cgcgcccgac
     3541 cgcacaggtc ctgacctcga accgcggaat ggtcgtcgcc gccgccccgg aggcgtcgcg
     3601 ggcgggcgcg gaggtcctcg ctgcgggtgg caacgcgatg gatgccgcgc tcgcggcggc
     3661 gctcgccctc gccgtcgtct atccgcaggc ggggaacctc gccggcggcg gcttcctcgt
     3721 cgtccgcacg cccgacggag tcgtccgggc gctcgatttt cgggagacgg cgccggcggg
     3781 cgcgtcgagc aggatgttcc tcgggaagga cggccggccc gtcccgggag cctccacgtc
     3841 gacggctctg gcggtcgcga cgccggcctc cgtgcgtggc tacgcggagg cgcatcgccg
     3901 cctgggccgt cttccgtggg ccgccgtcgt ggcgcccgcc gagaggctcg cccgagaggg
     3961 gttcgtcatc ccggacgggc tctcgcacga cctcgaggac gagcgcgggc ttctcacccg
     4021 ctgggacgag acccgccgcg tcttcttccc gggcgggcgg cccctcgctc cagggacccg
     4081 gctgaagcag ccggagctcg ccgagacgct cgcgcggatc gggcgagagg gcgccgacgc
     4141 gttccacaga ggcgagatcg cggcgcggat cgcggcgttc gtgcgggagg cgggcggcat
     4201 tctctcggag ggcgacctcg ccggttacgc cccggtctgg cgggcgcccg aggagatccg
     4261 attcggtgac ctcgtcgtcc acacgatgcc ccttccgtcc tccgcggggc tcgtcctccg
     4321 gtcggtcctc gcgcagctcg aggtggctcg cggtccgggc gccttccgcc cggacgcggc
     4381 cgcataccac gtcctcctcg aagcggagcg tcgggcctac gcggatcgga accgctggct
     4441 cggcgacggg gactgcgtcg aagtgccgct tcccgagctg ctcgagccgg agcgtctcgt
     4501 ccggctcggt gcgtcgatcg accacgagcg ggcgacgccc tccggctccg tcccgggcgg
     4561 gctcgcgcgc gagcgggagg agacgacgca cctttccgtg gcgacgccgg acggcttcgc
     4621 ggtctcgctg acgacgacgc tgaacggctc gttcgggaac ggcgcgatcg tcccggggac
     4681 cggcgtcctg ctcaacaacc agatggacga cttcgccgcg gcgccgggga ccccgaatat
     4741 gtacggtctc gtgcagggga gcgcgaacgc ggtgcgtgca ggggcgcggc ctctttcctc
     4801 gatggcaccg gccgtcgtcg agaaggctgg gcgaccgttc ctcgtcgtcg gctcgccggg
     4861 gggctccacg attccgacga ccgtgctcca ggtccttctc cggacggccg gcggggagga
     4921 cctcggctcg gctgtcgcgg cgccgcggct ccaccatcag cacacgcccg atgtcgtctt
     4981 cgtcgagcgg ggccgcgcgg cttccgaggt cctcgagggg ctccgtcggc gaggccacgc
     5041 cctgagggag cgcggtccga tcggcaaggt ccacgccgtc tccttctcac cggacggtcg
     5101 cctcgtcggc gcggcggacc cgagaggcta cggagcgagc gcggcgccct gagtccgcag
     5161 cggtcgacgg acggagcctt ccggcccctt ctccgtcttc ccttccgtga aggcccgtgc
     5221 tatcttccgc gtccgcgctt ccgtcggggc gtagcgcagc ctggtagcgc acacggttcg
     5281 ggaccgtggg gtcggaggtt cgaatcctct cgccccgacc atcatcgcga tcgacgaccc
     5341 cgcctcccga gcggggtctt tttccgttag gcttcccgtg cgcggtccgg tcgccgcggc
     5401 agggaggacg acgcgatggg caacggcggc gtctcggtca cggtcgatgg caggtccgcg
     5461 acggtcacgt tctggagtcc acccgagaac gtcctcgacc gggagaccgt cgtcgccctg
     5521 gagtcgcggc tgcgggaggt cgcccgccgg cgcgacgtga gactggtggc gctcgcggcg
     5581 cggggagagg acttctgcaa cggggccgag gtcgttccgc ccgtcgacgg acgaccccag
     5641 gctctcctcg aggcgtggca ctccctcgtc cggttcctcc tcacctgcga ggtcccgacc
     5701 tgtgccctcg tgcaggggcg tgcgctcggc gccggcgcgg agctcgcgct cgcctgtgat
     5761 ttcatattcg ccgagacgac cgcgtcgttc gggttcccgg agattcaccg cggctcgttc
     5821 ccgcccgtcg cggcggtcct tctcgagcgc cgcgccggcc ggacgaaggc cgcggacctg
     5881 atcctctgcg gcgccgcgat gaccgccgag gcggcggagc ggcgggggct catcaacgcc
     5941 ctcgtgaacc cgggcgacct gatcgaggcg ctcgagacga tgaagacgag gctggagact
     6001 ctctcggcct cctcgctccg tctcgcgaaa cgggcgctca accaggggtg ctcgggcgat
     6061 ctcctctcgg cgctctcggc ggtcgagcgg accttctccc gcgagctgtt gaagacggag
     6121 gacgcggcgg agggggcaag agcggcgctc gaaggccgtc cgcccgtttg gacggaccgg
     6181 taggagaagg aacgccccgc cgcgggaggg acccgggcgg ggcgtaggga ggatccggcg
     6241 ccgcggaggg gcgcggcgcc ttccggggtc agggaggaga gagcggaact ggaatcggac
     6301 tcacccttcg tccggtagtg tcggaacccc gaaggaacta acgcggtggc gccgaaggct
     6361 gtggcgcgac ctcgaggagg gtcgagaacg tcggcgttcc gccgtcccag agcgtcacgc
     6421 cgctgcgccc ctcagcggga acgcccgccg gaagccggac ggacatgtcg gccgacgccc
     6481 cggacggaac ggcctggtcg ccgctgtccc aggtgaccgc tccggactcc tcccggagaa
     6541 cgaccctcca gctccgggcc tcgcccgaaa gaaggtggcg cggaaagacg agcaggagcg
     6601 gcgaccccgc gaggcgctcc acgcggcgga cggagacgcc gactccctcg cccggctgca
     6661 ggacgaccga cggggtcgac ggcaccacac cgccccccgc cgccgtcacg acggccggcg
     6721 ggacggtcga gagctcggcc cggagcgtgg cgagctgcgc cttcagccgg agcgccgtca
     6781 cgagcgccgc gaggaggagc acgcagagga ggccgaacgc gacgacgacc ccggtgcgac
     6841 ccgggaagag tcctcccccg gagcgggggg gcgcgcctcg gccgaggctc cggactttgt
     6901 ggtcgcgggc gatccggcgt cgaagccgcg tcgtcgtttc aacgcgctca cgatagtagg
     6961 gagtcccgag cagcgcctgc tcgaaccgcc gccggtcctc gtcggacagc cggcccagga
     7021 cgtagtcggt gacgaggaga tcctcctcgg cctcgacgcg atcgaggagg gcgtcgtcgg
     7081 cgaagtacga ctcctcgaag gtctggcgct cgtcgtcccc gagacgccgc aggaggtagc
     7141 cgcggatccg gccgcggtcg acggcccctt cgcggctcac gcctcacctc cgtcttcgcc
     7201 cgggccgcgg cgggcctctt cctcgcgctg tgcccggacg gcggagcgcg cggccgtcga
     7261 atccatcttc ttccgaagct cgcgcttggc gcggtagacg cgcatgtcga cggcgtcggt
     7321 cgtgatcccc tcttcgcggg cgatcgtccg cgaagggacc tcctcgacga agcggcggac
     7381 gagcagctcc ctcatccggg caggcaggag ctcgagcgcc ttccggacga tgaggcgccg
     7441 gctgctcgag agcgcgtcgg agagcgggtc ctccgtcccg gcgagctcga ggacgcccga
     7501 aagtccttcg tgccgattcg ccttctggac tcgccggcag tactcgaaga agacgttctt
     7561 ggcgacaccg agggcgaaag cctcgggaga ctccagcggc tcgccgcggc tcgcccgctg
     7621 aaggactcga agcagcgtct cctggacgag gtcgcgggct tcgtcttccg tccggacgcc
     7681 gcgggagagg aagaaacgcc ggagtttctc ggggagtcgt ccgagctcgg cctcgaaggg
     7741 gtccgcccag gaagtcgtca tcgttcttcg catcgagaaa gcaagcggcg ggccgtaccg
     7801 ctcgtcatca cgagaggatt ctagtccagg agcgatcggc atgcgtgtgc tccgaatagg
     7861 attcggcact aatcagaaat ttccgaattt catacggcat atgatgggta tgggccgcaa
     7921 tgatgaggct tctcgttgcg cccgcgcggc acgtcggcgc tccgggtgtt cggtcgggcg
     7981 ggcttgactt gcgcgagaat tgcgccatac tctccagcag gaccggtcgg agcttccgaa
     8041 cgaaattagg atctttggcg aaagtgacga atgaattatg gcaaatcgtc caaatctcac
     8101 ggagagacag ctggagatcg tcgcattcat tcgggactac cgggcgcggc gaggtatcgc
     8161 gccaacccag cgcgagatct gcgagcactt cggctactcc tccttcggca cgcttcagaa
     8221 gcacatccgg ctcctcctcg acaagggagt gctcgtacgc gactggaacc agcgccgctc
     8281 cctcgacgtg gccgaagagg ccgcctcggc tcccgcgctc gaagtccccc tcctcggccg
     8341 gatcgcggcc ggcagcccga tcgaagtcct cccgggtgac gaccggcttc acgtgccgga
     8401 gtcgctcacg cgcaaagggg agaacttcgt cctcctcgtc agcggcgact cgatgatcga
     8461 cgaggggatc cgggacggcg actgggtcgt cgttcatcgg cggacgaggg ctgagaacgg
     8521 cgagatggtc gcggcgctcg tgggcgggga ggcgacgctg aaacgcctgt atcgggaagc
     8581 cgagggcatc gtccggctgc agccgtcgaa cgagcggctc gctccgatcc gtgcccggga
     8641 agaggacgtt caggtccagg gaatcgtcgt cggcctgatg cgccggtact gacccggacc
     8701 cggcaagacc ggaggccgtc aggaacggca gccgatcgcg ctcgccacgg ctgtgccggc
     8761 gctcacttga cgggttcgtc ggcatgcccc atattccgac catcctcaga ggaaccctcg
     8821 ccgtcgcctg atgtaggcgc gcgaaaggca tttctcggcg gaagaccaca acctgttgaa
     8881 agatggagat ttatggaccg acgagatgaa gaaccgatcg aagcgagcga acggcgcagc
     8941 cggggagcaa tcctccggct ggcgggcgcg ggcttcgctt cgattctcgc tatcgccgtt
     9001 ccgctggacg ggagcgccgg cgtcagcgcc ggaggaccgg tcgcgccggc tccggacgac
     9061 gctccggcgc cgacgacgct gccgcccgga tggcccggag agacgccgga cctcgcgacc
     9121 ccccggcagc tcgacgtctg gtcgatcgtc cacgccgcct gccggaggca cgggatcgag
     9181 gacgaggcgg tcaccatgta ccacgtcctc tgggaggaga gcaccctgcg ggggcatgtc
     9241 agctccccct gcgggcgcta ccacggcatc gggcagttca ccctttcgac gttccgggag
     9301 agcgttggaa agatgcgccg ccttcggctg atctggggcg acgaggagtg gtcgccattc
     9361 gacccggtcc aggcgatcga ggtgatggcc ttcatgtgga gccggggcta ccacgaccac
     9421 tgggggccgt atcgtcgcgt cgtccgcagg caggcccggg ctctggcggc cgcgagcctg
     9481 aactgaccgg ccacgtcccg tcgcgtggct ccggcggctc ggtgagggcc ggcggggttc
     9541 acgcgccggt cgagccgaag agaaccatcc cgtagtcgcc ggtgacccgg aacccgagcg
     9601 cgaggtaggc ccggcgggcc ggctcgttct cccgctccgt gaagaggacg gcgcgcgaga
     9661 cccccgccgc gcgggcggcg aggagcgatc cggcgacgac ggcccgcgcg tggccccgcc
     9721 cgcgcagctc cgaaggagtg aagaccccgc ccacctggac gacctccgga agccgcgcgt
     9781 tgaagccgct gaaggcgacg gggaccccgg cccgacggag gacgaaggcg ttcccttcca
     9841 cgacgaggcg gtcgacctcc tcggcgcacg ccgccggcag gccctcgccc gggacggcgc
     9901 cgagggtttc gacggcgtag gcgacgcgcc agtcgacgag gagcgaccgg tcctcggggg
     9961 acgggggagc gcaggcgacg tctccggtcg agaggccctc ggggacgacg aggccgtcga
    10021 gggcgaggga gaagagctcg gcctcgtggt cgagcgcggc gggcgccccg tcgagcccga
    10081 gcgccgcccg ggccgcgacg acctgccccc gggggcccga aaggccggcg agagggcggc
    10141 cggaggcgga gagcgccgcg cgggcgagct gggcgaggcc ccgcggggcc tggagaagga
    10201 gaaccccgtt ccagcagtgc gcggcgacgc cggcgacgta agggccgtcg aacgcggcga
    10261 cccaggtcgc gctgtaggcc gctcccgtgt cggcgagccc cgcggcccgc aggttcccga
    10321 ggaggaagag cgacgaatcc gcgtgtgctc ccaggaacgc ccggacgaga gcctcgtcgc
    10381 ccggtccgag gacgcgcggt agaacgttca cggacggggc gccgggagcg tcgcggccgg
    10441 tccgacgaga tggacggtcc gggtcgggaa cgcgaagctc gtcccggcct tttcgacgat
    10501 ctcgaggatc ccgatcaggg cttcctgccg gatgtcccgg aactcgtcga agctcgtcgt
    10561 ctggaaccag gccatcacct cgacgtccag ggaggagcct gcgaaggcga cgaaccgcac
    10621 gacgacggtg tcgggccaga tcttcgggtg ggcccggagc atcgcctcga ttcccgagac
    10681 gacctcgcgc atctgcgcgg cggtcgtccc gtactcgagg ttcaggatcg tggcgaggcg
    10741 gatccggtcc cgttccgcga acgtctcgat ctgcatgtcg gcgagcttac cgttcgggat
    10801 cgtgacgagc gtccggtcga gcgtccggaa gcgggtcgac cgaagcccga tcttctccac
    10861 ggtcccgagg acgtccccca ccttgacgaa gtccccgacg cgcagcggct ggtcgacgcc
    10921 gagc
//
LOCUS       DAOONL010000111        10911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6797_length_10911_cov_15.386422, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000111 DAOONL010000000
VERSION     DAOONL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_10911_cov_15.386422"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLP50_13505"
     CDS             <1..254
                     /locus_tag="PLP50_13505"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HPA52605.1"
                     /translation="MSQKTVEHIIGRLATDEEIRLRFRKDAVATVRAAVSEPEALTSV
                     EQEALASIDPDVLDRLADAIDPRLQRVRIPAARGDGRRR"
     gene            251..1855
                     /locus_tag="PLP50_13510"
     CDS             251..1855
                     /locus_tag="PLP50_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA52606.1"
                     /translation="MRPQAAFLLLAALPAIAQPADDAGPELRLTLAEARAIALERNVD
                     LRVERETAATSEAGEVRARGAWDPLVRVDARLRERTDPVNSILSGAPSGELAPTQRSA
                     GLSLGVVQLLPTGGSLTLTSTLAREKTDSVFSILSPSWTSSLGLEIRQPLLRNRATDV
                     ARTALRVARVDRGRSAASLQRVAADTVAALERSYWTLVAARQSVEARTSAVALAVRQQ
                     EDVSARVEAGTLSESDLASPLAEVERRKGELLASREAAARAENALKSLLLGDPADPAW
                     ASRVVPADDAEPAAAPAAPTAPDVAAAEGRRPEVAEAAARLDRLTIEAEAARDRVRPQ
                     LDLVAGYIGRGLAGDPNPGVAPPFPSGPVEVPEALDGGLGRSLGTIGEGRFPDASVGL
                     AFALPLGNRAARAESRQLRSARVQAELALLRARQQVGVEVRNAAFAEETAAQRVAAVR
                     AARLAAETQLRAEEERFAAGLTTSFFVLTRQNDLTQARLVETQALADLRMARVERARA
                     EGTLLSDRRIQLHASEPAAAPERGTR"
     gene            1852..2886
                     /locus_tag="PLP50_13515"
     CDS             1852..2886
                     /locus_tag="PLP50_13515"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52607.1"
                     /translation="MNRDLRCFAAAVFLPLLLAGCREAGDGAATRLNGRIEAPVVDLA
                     PKVSGRVLEVKVREGDRVKAGDLLVTLDLGEIALSVERDRDALGSASARLRDLQSGSR
                     SQEVAAAEADLADKKAALELARKELARQEALMAKKVGIPRDLDRARTELERTIAARSA
                     SAERLALAREGFRRFQTEGARADVRRAETILKQSETIVREAEILAPADGVVLHRMAEP
                     GLLLGAGQPALTLAFADRLYVRTFIPETRLGRVRPGLAAQVFVDAFPGRSFPARVTEI
                     SPTAEFTPKAVETREERVNLVYAAKVDLDAGWDAPLVPGQPAEVVVETDGEAPTVTTK
                     PPAVPSASLR"
     gene            2894..3784
                     /locus_tag="PLP50_13520"
     CDS             2894..3784
                     /locus_tag="PLP50_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52608.1"
                     /translation="MIEARGLEKRFGAHAALRGVDLSIAEGELFALIGPDGAGKTTFF
                     RLVAGLITPTAGTLRRAEGATFGLVPQRFSLYEDLSVDENLDLRARLYSVPPAEARTR
                     ASELLARVGMSPYGARLAGALSGGMKQKLALVAALLTRPRLLLLDEPTTGVDPVSRRE
                     FWLVLNELHHEGLTIVVSTPYMDEAEYATRLGFLDEGKLLAVGTRDEVLAAFPRPILE
                     VLSPDRNGVRQRLAGLDGVDDVSLFGTRLHLRGAAGTGPEILDSVCAGLEGLVPADGI
                     RIVPPTLEDVFVLAGDEVGE"
     gene            3873..4865
                     /locus_tag="PLP50_13525"
     CDS             3873..4865
                     /locus_tag="PLP50_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52609.1"
                     /translation="MRAETSSRAPLPPEVFLRAEGLVKRFGDFVAVNGVSFDVPRGKV
                     FGFLGPNGSGKSTTIRMLAGLLNPTSGSARGFDGLDVTDTERWKSRLGYMSQKFSLYL
                     DLTVEENLRFFGRVYGLAGPRLDERIRELSGALRFERLKDTLTESLSTGMRQRVALAA
                     ALLHEPLLLFLDEPTGGVDPKGRRLFWELIYDLAAEKGMTVLVTTHYMDEAEQCDELA
                     FILNGDLIAHGEPQALKRGISGRLLEVDLESAPFEALAAVRGAAPLEDAYLFGLRLRV
                     LAREGCVDDALSLVSRFGTPATAEPSLEDVFVDLARGYAERKNAAQPPTRMVTT"
     gene            4862..5977
                     /locus_tag="PLP50_13530"
     CDS             4862..5977
                     /locus_tag="PLP50_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52610.1"
                     /translation="MNRLLALMRKEFLQLRRDTITLRMMVFIPVMQTFIFGYAINYDV
                     KHLKTIVLDEDRSFDSRELVAKMAASQYFDVVGTVGSFDELRREMDEGRVSSALVIDR
                     DYGESRRRGVPARALLIVNASDTTTSTQAMSIAAGISNQLSVQTLARRAGWKEASLPV
                     DLRVRPWYNPDLRTATFIIPGLIAIILTFTLIPFTAIAIVRERERGTLEQLQVTPVTR
                     LEIILGKLFPFILIGVFQLTLIIALMVFLFRVPIAGSVLQLYLSGLVFMAAVLGLGML
                     VSTVAKTQMQAMQLSFVFLLPFVFLSGYVFPIDGMPKVFQLITYVIPARYFIEVIRGI
                     VLRGASLPELWKPMAWMTAYTVLIVAAAVLRFKKTND"
     gene            complement(6050..7297)
                     /locus_tag="PLP50_13535"
     CDS             complement(6050..7297)
                     /locus_tag="PLP50_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HPA52611.1"
                     /translation="MLDLEAIRAKFPALASGDVVLDNPGGTQVPREVTDRIVRYLHET
                     NANHGGAFTTSRASDAVVAEARAAVADFLGATRPEEIAFGPNMTTLTFQVSRSLSRDW
                     RPGDEVVLTRLDHDANVSPWLLAAAEKGATVRWLDFDPADCTWSVDELRKLLSERTRL
                     VAVGWASNSVGTVNDVAGAAKAAHEVGALVYVDAVHYAPHGPIDVAEAGIDFLACSAY
                     KFFGPHLGILWGRHDLLEKTFAYKVRPAGDAPPDRWETGTQSFEAVAGTLGALEYLGW
                     VGERSGGPYREALSGRYAGRRLVLKSAMAAIHAYEARLSRALLEALADVPGLTIRGIS
                     DPALFAARVPTFSFTMDGRHPRAIAEELGRRGFRVWDGNYYAVGVTERLDLEASGGMV
                     RVGPVHYNTVDEIRRLGAALRSL"
     gene            complement(7321..8676)
                     /locus_tag="PLP50_13540"
     CDS             complement(7321..8676)
                     /locus_tag="PLP50_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPA52612.1"
                     /translation="MKPRSGDLFEEPIADRRAVPSVAPLAERFRPRDLSEMRLPASVA
                     GPSSLLGRAISADRVPSLVLWGPPGTGKTTLARVIATRTKARFLSFSAVVSGVKEIRE
                     VLDESQRLAARGERTLLFVDEIHRFNRAQQDVFLPYVESGAATLVGATTENPSFELNG
                     ALLSRMRVVVLPPLSREDLESILRSAAQDAERGLGGRVTLGEGAVDWLVGFSDGDARR
                     ALNALETAADHAGEGAVLTAALLGEIYARKSLLFDKSGEEHYNLISALHKSVRDSDVD
                     ASLYWLARMLEAGEDPLFVARRLVRASTEDVGLADPNALRLAMAARDAVHFLGMPEGA
                     LALAEATVYLALAPKSNALYMAYGEAARDVAETPNAPPPRTILNAPTRLMKDVGYGKG
                     YVYAHDTAEGTAGLSCLPDALAGRRYYRPKGTGREAEAKRRLDELHELRERLRRERHR
                     R"
     gene            complement(8673..9314)
                     /locus_tag="PLP50_13545"
     CDS             complement(8673..9314)
                     /locus_tag="PLP50_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52613.1"
                     /translation="MALPAPRWGDRLLFAALGALVGFAGAWIVLDGRHAHAPQPPAMA
                     MDDPHAGVPGAPPLGPGASAPAAGGSTFDRAAWQRLQDAQAAATAKPDDYDALVQLGN
                     AAYDAHEYAQAIEAYERALKVRPGDPNVMTDLGTAYRNEGKLDEALVLFRKARAADPT
                     HWQSLYNEVVVLALDKHEHDAADAALARLKKEHPGLPAIETLERQIGAAHGGS"
     gene            complement(9395..10147)
                     /locus_tag="PLP50_13550"
     CDS             complement(9395..10147)
                     /locus_tag="PLP50_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52614.1"
                     /translation="MSHRAPAGLLLVLALGVPLAAAARTDPSMESLLVALDLERRARN
                     EDVLEVERLTSAATRADAAAASARERLVDAVRGGDVQAGDLRDEEERVVEAEARAAAA
                     REVRRAAVARLVERVRRIALLQEEVVRRRSASRRAADPITGRWEATIDPGARKGAFRL
                     VLDGTLVSGDYTLDGGFRGSLRGTYVGERITIQRIDSERGFDATFYGRVQPQLKRITG
                     TWEATAIAPAIGPVAGTWSAALLPDDEDGGQP"
     gene            complement(10197..10718)
                     /locus_tag="PLP50_13555"
     CDS             complement(10197..10718)
                     /locus_tag="PLP50_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPA52615.1"
                     /translation="MGFNIGDKLVYPNHGVGVVETIGESLFDGRAHPCYQVRLLANNS
                     KVVVPVGNSDRVGLRPLTRRQDVTGVFKVLEDGSFQSNGDWKGRFKQNLDKMRSGRLS
                     DIADVLKNLNWVQKQKTLSFREKKMYERARYLIVSEIAQVNGLPEAEVELDVEKALDR
                     SVARRRSLGPAAR"
BASE COUNT         1478 a         4055 c         3831 g         1547 t
ORIGIN      
        1 agatgagcca gaagaccgtc gaacacatca tcggccggct cgccacggac gaggagatcc
       61 ggcttcgctt ccggaaggac gccgtcgcga ccgtccgggc ggccgtgtcc gagcccgagg
      121 cgctcacgag cgtcgagcag gaggccctcg cgtcgatcga tccggacgtc ctggaccggc
      181 tcgccgacgc catcgatccc cgcctccagc gggtcagaat tcccgcggcg cgtggcgacg
      241 ggaggagacg atgaggccgc aggcggcgtt cctcctcctc gcggccctcc ccgcgatcgc
      301 gcagccagcc gacgacgccg gcccggagct tcgcctcacg ctcgccgagg cccgcgcgat
      361 cgcgctcgaa cggaacgtcg acctccgcgt cgagcgggag accgccgcca cctcggaagc
      421 aggcgaggtc cgagcgcgcg gagcctggga cccgctcgtg cgggtcgacg cccgcctgag
      481 ggagcggacc gacccggtca attcgatcct ctccggggcg ccctccggcg agctcgcgcc
      541 gacgcagcgg agcgccggcc tctccctcgg cgtcgtccag cttctcccga ccggcgggtc
      601 cctcacgctc acctccacgc tcgcgcgaga gaagaccgac agcgtcttct cgatcctctc
      661 gccttcctgg acgagctcac tcgggctgga gatccggcag ccgcttctgc gcaaccgcgc
      721 cacggatgtc gcccgcaccg cgctccgcgt cgcccgcgtc gaccgcggcc gctcggcggc
      781 ctccctccag cgcgtggccg ccgatacggt cgcggcgctc gagcgctcct actggacgct
      841 cgtcgccgcg aggcagtccg tcgaagctcg aacgtccgcc gtcgctctcg cggtgagaca
      901 gcaggaggac gtgagcgccc gcgtcgaagc ggggacgctc tccgagtcgg acctcgcctc
      961 gccgctcgcc gaggtggagc gccgaaaggg ggagctgctc gcctcccgcg aggccgccgc
     1021 ccgcgccgag aacgccctga aatcgctcct cctcggcgac ccggccgacc cggcctgggc
     1081 gagccgcgtc gtccccgccg acgacgccga gccggcggcg gctccggccg ctcccacggc
     1141 tccggacgtc gcggccgccg aagggcgccg gccggaggtg gcggaggcgg cggcccgtct
     1201 cgaccggctc acgatcgagg ccgaggcggc ccgcgaccgc gtccgtccgc agctcgacct
     1261 cgtggccggc tacatcgggc gcggacttgc cggcgacccc aaccccggag tcgccccgcc
     1321 cttcccgagc ggtccggtcg aggtccccga ggcgctcgac ggcggcctgg gccgatcgct
     1381 cggcacgatc ggcgaggggc gttttcccga cgcctcggtc gggctcgcct tcgcgctgcc
     1441 gctcggcaac cgggcggcgc gggccgagtc gaggcagctt cgctccgctc gcgtgcaggc
     1501 cgagctcgcc ctcctccggg cccggcagca ggtcggcgtg gaggtgagga acgcggcgtt
     1561 cgccgaagag acggcggcgc agcgggtggc cgcggtccgc gccgcccggc tggctgccga
     1621 gacgcagctc cgagccgagg aagagcgctt cgccgccggc ctgacgacga gcttcttcgt
     1681 cctgacccgg cagaacgacc tgacgcaagc gcgtctcgtc gagacccagg ccctggccga
     1741 cctgcgaatg gcacgcgtcg agcgcgcccg cgcggagggg acgctcctct ccgaccgccg
     1801 gatccagctt cacgccagcg agccggccgc cgcgccggag agaggaaccc gatgaatcgc
     1861 gacctccgct gcttcgccgc ggccgtcttc ctgcccctcc tcctcgccgg ttgccgggaa
     1921 gccggcgacg gcgccgcgac ccgtctgaac gggcggatcg aagcccccgt ggtggacctc
     1981 gccccgaagg tctcgggccg cgtcctcgag gtgaaggtcc gcgagggcga ccgcgtgaag
     2041 gcgggcgacc tcctcgtcac gctggacctc ggagagatcg cgctttccgt cgagagagac
     2101 cgcgacgccc tcggctcggc ctccgcgcgc ctccgggacc tccagtcggg cagccggagc
     2161 caggaggtcg ccgccgccga ggccgacctc gccgacaaga aggcggccct ggagctcgcc
     2221 cggaaggagc tcgcccgcca ggaggcgctg atggcgaaga aggtcgggat cccgcgggac
     2281 ctcgaccggg ccaggaccga gctcgagagg acgatcgccg cccgttcggc gagcgcggag
     2341 cgcctcgccc tcgcccgcga gggattccga cgcttccaga ccgagggtgc gcgagccgac
     2401 gtccgtcgcg ccgagacgat cctgaagcag tcggagacga tcgtccgcga agccgagatc
     2461 ctcgcgccgg ccgacggcgt cgtcctccac cggatggccg agccgggcct cctcctcggc
     2521 gcgggtcagc ccgcgctgac cctcgcgttc gccgaccggc tctacgtccg cacgttcatc
     2581 cccgagaccc ggctcggccg ggtgcggccc ggactcgccg cgcaggtctt cgtcgacgcc
     2641 ttccccggcc ggtcattccc cgcgcgcgtc accgagatct cccccactgc cgagttcacg
     2701 ccgaaggccg tcgagacgcg agaggagcga gtcaacctcg tctacgcggc caaggtcgac
     2761 ctcgacgccg gctgggacgc cccgctcgtc cccggccagc cggcggaagt cgtcgtcgag
     2821 acggacgggg aggcgcccac cgtcacgacg aagccgccgg cggtcccgtc ggcctccctc
     2881 cgctgagccg gcgatgatcg aagcccgcgg cctcgagaag cgcttcggcg cacacgccgc
     2941 ccttcggggc gtcgacctgt cgatcgccga aggcgagctc ttcgcgctga tcggccccga
     3001 cggcgccggg aagacgacgt tcttccgcct cgtcgcgggg ctcatcaccc cgaccgcggg
     3061 gacgctccgg cgcgcggaag gggccacctt cggcctcgtc ccgcagcgct tctcgctcta
     3121 cgaggacctc agcgtcgacg agaacctcga cctgagggcc cgcctctact ccgtgccgcc
     3181 cgccgaggcg cgcacccgcg cgtccgagct cctcgcccgc gtcggcatgt ccccgtacgg
     3241 cgcccggctc gccggggcgc tctcgggcgg gatgaagcag aagctcgcgc tcgtcgccgc
     3301 gctcctgacg cgcccgcgcc tcctcctcct ggacgagccg acgaccggtg tcgacccggt
     3361 ctcgcgccgc gagttctggc tcgtcctcaa cgagctccac cacgaggggc tcacgatcgt
     3421 cgtctcgacg ccgtacatgg acgaggcgga gtacgcgacc cggctcgggt tcctcgacga
     3481 ggggaagctc ctggccgtcg gcacccgcga cgaggtgctc gcggctttcc cccgcccgat
     3541 cctcgaggtc ctctcccccg accgcaacgg cgttcggcag cgcctcgccg ggctcgacgg
     3601 cgtcgacgac gtctccctct tcggcacgcg gctccacctt cgcggcgccg ccggcaccgg
     3661 gccggagatc ctcgactccg tctgcgccgg cctcgaaggc ctcgtcccgg cggacggaat
     3721 ccggatcgtc ccgccgacgc tggaagacgt gttcgttctc gctggcgacg aggtggggga
     3781 atgacgcata ccgcaacggg ggggccgggg ggcgtgcgga gccagccccc cggcggcgac
     3841 gtgttcgttc tcgcgggcga cgaggtgggg gaatgagagc ggagacctcc tcccgcgcgc
     3901 cgcttccgcc ggaggtcttc ctccgcgccg aaggcctcgt caagaggttc ggagacttcg
     3961 tcgccgtgaa cggcgtgtcg ttcgacgtgc cgcgcgggaa ggtcttcggg ttcctcggcc
     4021 cgaacggctc cggcaagtcg accacgatcc ggatgctcgc cgggctcctc aacccgacgt
     4081 ccgggagcgc ccgcggcttc gacggcctcg acgtgaccga caccgagcgc tggaagagcc
     4141 gcctcggcta catgagccag aagttctcgc tctacctcga cctgaccgtc gaggagaacc
     4201 tccgattctt cggacgcgtc tacgggctcg ccggcccgcg gctcgacgag aggatccggg
     4261 agctctccgg cgcgctccgc ttcgagcgcc tgaaggacac cctcacggaa tcgctttcga
     4321 ccggcatgcg ccagcgcgtc gcgctcgcgg ccgcgctcct ccacgagccg ctcctcctct
     4381 tcctcgacga gccgaccggt ggcgtcgacc ccaagggccg gcggctcttc tgggagctca
     4441 tctacgacct cgccgccgag aaggggatga cggtcctcgt gacgacccac tacatggacg
     4501 aggccgagca gtgcgacgag ctcgccttca tcctcaacgg cgacctgatc gcccacggcg
     4561 agccccaggc gcttaagcgc ggcatctccg gacggctcct cgaggtcgac ctcgagagcg
     4621 cccccttcga ggcgctcgcc gccgtccgcg gggcggcgcc tctcgaggac gcctacctct
     4681 tcggcctgag gctccgcgtc ctcgcccgcg aggggtgcgt cgacgacgcc ctctccctcg
     4741 tctcgcggtt cggcacgccg gcgaccgccg agccctcgct ggaagacgtc ttcgtcgacc
     4801 tcgcacgcgg gtacgccgag cggaagaacg cggcccagcc accgacaagg atggtgacga
     4861 cgtgaatcgc ctcctcgccc tgatgcgcaa ggagttcctc cagctccgga gggacacgat
     4921 cacgctccgg atgatggtct tcatccccgt gatgcagacg ttcatcttcg gctacgcgat
     4981 caactacgac gtcaagcacc tgaagacgat cgtcctcgac gaggaccgct ccttcgacag
     5041 ccgcgagctc gtcgcgaaga tggcggcgag ccagtacttc gacgtcgtcg ggaccgtcgg
     5101 ctcgttcgac gagctgcggc gcgagatgga cgaaggccgg gtctcgagcg ccctcgtcat
     5161 cgaccgggac tacggcgaaa gccgccgccg gggagtcccg gcgcgggcgc tcctcatcgt
     5221 gaacgcctcc gacacgacga cctccactca ggcgatgtcg atcgccgcgg ggatctcgaa
     5281 ccagctctcg gtccagacgc tcgcccgcag ggccggctgg aaggaggcct ccctccccgt
     5341 cgacctccgc gtgcgcccct ggtacaaccc ggacctccgg accgcgacgt tcatcatccc
     5401 cggcctcatc gcgatcatcc tgaccttcac gctcatcccg ttcaccgcga tcgccatcgt
     5461 gcgcgagagg gagcgcggga cgctcgagca gctccaggtc acgccggtca cgcgcctcga
     5521 gatcatcctc gggaagctct tcccgttcat cctgatcggc gtcttccagc tgacgctgat
     5581 catcgccctg atggtcttcc tcttccgggt cccgatcgcc ggaagcgtcc tccagctcta
     5641 cctctccggc ctcgtcttca tggcggccgt cctcggcctc ggcatgctcg tctccaccgt
     5701 cgcgaagacg cagatgcagg cgatgcagct ctcgttcgtc ttcctcctcc cgttcgtctt
     5761 cctctcggga tacgtcttcc cgatcgacgg gatgccgaag gtgttccagc tgatcacgta
     5821 cgtcatcccg gcccgctact tcatcgaggt gatccgcggg atcgtcctcc gcggcgcgag
     5881 ccttcccgag ctctggaagc cgatggcctg gatgacggcc tacaccgtcc tcatcgtcgc
     5941 cgccgccgtc ctgcgcttca agaagacgaa cgactagccc tcggggtcag gtctaccatc
     6001 tacactctgc tcgcgagcag agtgtagatg gtagacctga ccccccctct cagaggctcc
     6061 ggagggcggc tccgaggcgg cggatctcgt cgaccgtgtt gtagtggacg gggccgacgc
     6121 ggaccatgcc gccggaggcc tcgaggtcga ggcgctcggt cacgccgacc gcgtagtagt
     6181 tgccgtccca gacgcggaag ccgcggcggc ccagctcctc ggcgatcgcg cgcgggtggc
     6241 ggccgtccat cgtgaacgag aaggtcggga cgcgcgcggc gaagagcgcc gggtcggaga
     6301 tgccgcggat cgtgagaccg gggacgtctg cgagcgcctc gaggagcgcg cgggagagcc
     6361 gcgcctcgta ggcgtgaatg gccgccatgg cgctcttcag gacgaggcgg cgaccggcgt
     6421 agcgaccgga gagcgcctcg cggtacggcc ctccggaccg ctcccccacc cagccgaggt
     6481 actcgagggc cccgagcgtc ccggcgacgg cctcgaagct ctgcgtcccg gtctcccagc
     6541 ggtcgggagg cgcgtcgccc gcggggcgga ccttgtaggc gaacgtcttc tcgaggaggt
     6601 cgtggcgccc ccagaggatc ccgagatgcg ggccgaaaaa cttgtaggcc gagcaggcga
     6661 ggaagtcgat ccccgcctcc gcgacgtcga tcgggccgtg gggcgcgtag tggaccgcgt
     6721 ccacgtagac gagcgccccg acctcgtgcg cggccttcgc cgctccggcg acgtcgttca
     6781 ccgtcccgac ggagttcgag gcccagccga ccgcgacgag cctcgtcctc tccgacagga
     6841 gctttcgcag ctcgtcgacg ctccaggtgc agtcggcagg gtcgaagtcg agccagcgca
     6901 ccgtcgcgcc cttctccgcg gccgcgagga gccagggcga gacgttcgcg tcgtggtcga
     6961 ggcgcgtcag gacgacctcg tcgcccggcc tccagtcgcg cgagaggctg cgcgacacct
     7021 ggaacgtcaa cgtcgtcatg ttcgggccga acgcgatctc ctcgggccgc gtcgccccga
     7081 ggaagtcggc gacggccgca cgggcttcgg ccacgacggc gtcggaggcg cggctcgtcg
     7141 tgaaggcgcc tccgtggttc gcgttcgtct cgtggaggta gcggacgata cggtccgtca
     7201 cctcgcgcgg gacctgcgtg ccgcccgggt tgtcgaggac gacgtctccc gaggcgaggg
     7261 cggggaactt cgcgcggatc gcttcgaggt cgagcatgcg cgaaggttag cgcggagcgg
     7321 tcagcggcgg tgcctctccc gccggagccg ctcccggagc tcgtggagct cgtcgaggcg
     7381 gcgcttcgct tcggcctccc tcccggtccc cttcggccgg tagtaccggc ggccggcgag
     7441 ggcgtcggga aggcaggaga ggccggcggt cccttccgcg gtgtcgtggg cgtagacgta
     7501 ccccttgccg taaccgacgt ccttcatcag cctcgtcggc gcgttcagga tcgtccgggg
     7561 cggcggcgcg ttcggcgtct cggccacgtc ccgcgcggcc tcgccgtagg ccatgtagag
     7621 cgcgttcgac ttcggcgcga gcgcgaggta gacggtcgcc tccgcgaggg cgagcgcgcc
     7681 ctccggcatt ccgaggaagt gcacggcgtc gcgcgcggcc atcgcgagcc ggagcgcgtt
     7741 cgggtcggcg aggccgacgt cctccgtcga ggcccgcacg agccgccgcg ccacgaagag
     7801 cgggtcctcc cccgcctcga gcatccgcgc gagccagtag agcgaggcgt cgacatcgga
     7861 gtcgcggacc gacttgtgga gggcggagat gaggttgtag tgctcctcgc ccgacttgtc
     7921 gaagaggagc gacttcctcg cgtagatctc gccgaggagc gccgccgtca ggacggcccc
     7981 ctccccggcg tggtcggccg ccgtctcgag cgcgttcagg gcgcggcgtg cgtcgccgtc
     8041 ggagaagccg acgagccagt cgacggcccc ctcgccgagc gtcacgcgcc cgccgaggcc
     8101 ccgttcggcg tcctgggcgg cgctccggag gatcgactcg aggtcctcgc gcgacagggg
     8161 cgggaggacg acgacgcgca tcctcgagag gagcgcgccg ttcagctcga acgacgggtt
     8221 ctccgtcgtc gcgccgacga gcgtcgccgc gcccgactcg acgtacggga ggaagacgtc
     8281 ctgctgggca cggttgaacc ggtggatctc gtcgacgaag agaagcgtcc gctccccgcg
     8341 cgcggcgagg cgctgagact catcgagcac ctcccggatc tccttcactc ccgagacgac
     8401 ggccgagaag gagaggaagc gcgccttcgt ccgcgtcgcg atgacccgcg cgagcgtcgt
     8461 cttccccgta ccgggcgggc cccagaggac gagggagggg acgcggtccg ccgagatcgc
     8521 gcggccgagg agggacgagg ggccggcgac cgacgccggg agccgcatct ccgaaaggtc
     8581 gcgtgggcgg aagcgctcgg cgagcggcgc cacggaaggc accgcccgcc ggtccgcgat
     8641 cggctcctcg aaaaggtcgc cgctccgcgg cttcacgacc ctccgtgcgc ggcgccgatc
     8701 tgcctctcga gcgtctcgat cgccggcagg ccggggtgct ccttcttcag ccgcgcgagc
     8761 gcggcgtcgg ccgcgtcgtg ctcgtgcttg tcgagcgcga ggacgacgac ctcgttgtag
     8821 agcgactgcc agtgggtcgg gtcggcggcg cgcgccttcc ggaagaggac gagcgcctcg
     8881 tcgagcttcc cctcgttccg gtaagccgtg ccgaggtcgg tcatcacgtt cgggtcgccg
     8941 ggccggacct tcagagcccg ctcgtacgcc tcgatcgcct gcgcgtactc gtgcgcgtcg
     9001 taggcggcgt tcccgagctg gacgagcgcg tcgtagtcgt cgggcttcgc ggtcgcggcg
     9061 gcctgggcgt cctgcaggcg ctgccacgcg gccctgtcga acgtcgagcc tccggcggcc
     9121 ggtgcagacg cccccggacc gagcggcgga gcgccgggca cgccggcgtg agggtcgtcc
     9181 atcgccatcg ccggaggctg tggcgcgtgc gcatgacgcc cgtccaggac gatccacgcg
     9241 ccggcgaacc cgacgagggc tccgagcgcg gcgaagagga ggcggtcgcc ccagcggggc
     9301 gccggaaggg ccactgcggc ggccggcttc gatcgagccc cgtcccgggg tccggtccgg
     9361 gcctcgggcg gggggggaag atggtcgtcg tggctcaggg ctgacctccg tcttcgtcgt
     9421 cgggaaggag cgccgccgac caggtgcccg cgaccgggcc gatggccggc gcgatcgccg
     9481 tcgcctccca ggtgccggtg atccgtttca gctgaggctg gacgcgcccg tagaacgtcg
     9541 cgtcgaagcc gcgctccgag tcgatccgct gaatcgtgat cctctcgccg acgtacgttc
     9601 cccggagcga gccgcggaag ccgccgtcca gcgtgtagtc gccggagacg agagtgccgt
     9661 cgagaacgag gcggaacgcg cccttccgtg cgccggggtc gatcgtcgct tcccagcgcc
     9721 cggtgatcgg gtccgccgcc cggcgggagg ccgaacgcct ccgaaccacc tcttcctgga
     9781 ggagcgcgat ccggcggacc ctctcgacga ggcgggcgac ggcggcgcgc cgcacctccc
     9841 gggccgcggc ggcgcgggcc tcggcctcca cgacccgctc ctcctcgtcc cgaaggtcgc
     9901 cggcctggac gtctccgccg cggacggcgt cgacgagacg ctcccgcgcg gacgcagccg
     9961 cggcatcggc ccgcgtggcg gcggacgtga ggcgttccac ctcgaggacg tcctcgttcc
    10021 gtgccctgcg ctccaggtcg agcgcgacga gaaggctctc catcgacggg tcggtccgcg
    10081 cggcggccgc gagggggacg cccagcgcca ggaccaggag gagaccggcc ggagctcgat
    10141 gcgacatcgt cacgactata cgggagcggc ggcgcccgcg tcgccggatt cgaggctcag
    10201 cgggcggccg gacccaggct gcgacgccgg gcgacggacc ggtcgagcgc cttctcgacg
    10261 tccagctcga cctccgcctc cggcaggccg ttcacctgcg cgatctcgga gacgatcagg
    10321 taccgggccc gctcgtacat cttcttctcg cggaaggaga gcgtcttctg cttctggacc
    10381 cagttcaggt tcttgaggac gtcggcgatg tccgacagcc ggcccgagcg catcttgtcg
    10441 aggttctgct tgaagcgccc cttccagtcg ccgttcgact ggaagctgcc gtcctcgagg
    10501 accttgaaga cgcccgtcac gtcctgtcgc cgggtcaggg gtcgcagccc cacgcgatcg
    10561 gagttgccga ccgggacgac gaccttggag ttgttggcga gcagccggac ctggtagcac
    10621 gggtgggcgc ggccgtcgaa cagggactcc ccgatggtct cgacgactcc caccccgtgg
    10681 ttcggataga cgagcttatc gccgatgttg aaacccaccg gatccgcctc ctgcgctatc
    10741 ggacttgcta gcttacgcgg cccccggtcg ggggtcaatg ggatgccggt gcggggagtc
    10801 ccggcgggcc cgcgcggtgg gctcgacgcc cgggccgggc ctgcgcgaag cgtctggtgc
    10861 cggaggaggg actcgaaccc tcatgagctt gcgctcgggg gattttgagt c
//
LOCUS       DAOONL010000112        10887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_6826_length_10887_cov_11.364291, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000112 DAOONL010000000
VERSION     DAOONL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_10887_cov_11.364291"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLP50_13560"
     CDS             <1..589
                     /locus_tag="PLP50_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52616.1"
                     /translation="PLCCGRMNAQSRSRRWRAASLVLLTAAALASAPAASAQTFAIGA
                     GGGLLNDEGSAESLKNFSTGAGYGFAEIALDPGLLLQARYTRMKLPSRAADGPEIAVD
                     AATLSVAYLFREDWWQAGFVAGGGGYWLGPKEPGPGQVAVEQSASRFGWHGGVLTIFA
                     LGRKADLRLEAVGHLIRDDDRRKPIVLSAAVAWKF"
     gene            complement(966..1415)
                     /locus_tag="PLP50_13565"
     CDS             complement(966..1415)
                     /locus_tag="PLP50_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPA52617.1"
                     /translation="MDEIDRQILDMLQRDGKVSQARIAESVGLTTPSVNERIKKLETK
                     GFIRRFAALLDPRQVGMAAVAHVDVQLEHPSFERAFLDEIENLIPVQECYSISGDYGY
                     RLKVRARDLEQLEALLRERIQTIRGVARTRVDLSLSIKKDSTLLPVA"
     gene            complement(1488..2729)
                     /gene="glgC"
                     /locus_tag="PLP50_13570"
     CDS             complement(1488..2729)
                     /gene="glgC"
                     /locus_tag="PLP50_13570"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPA52618.1"
                     /translation="MLQHRDVLSIVLAGGMGERLYPLTRDRAKPAVPFGGRYRIVDFV
                     LNNFVSSGLMKIKVLTQFKSNSLIEHLVRAWRLNPEIGQFVDPVPAQMRRGPHWFRGT
                     ADAVYQNLDLIFDEEPSQVCVFGGDHVYVMDVNPMLAFHEDGDHDLTIAAFPVPVAEA
                     TRFGVLEVDDRGRVTGFEEKPREPREIPGAPGFALASMGNYIFRPKVLREALENDALQ
                     DTSHDFGRTIIPALLDGAASVFAYDFSLSRVPGDEELAPYWRDIGTVDSYWEASMDLI
                     SVSPPLNLYNPRWSLKSSSPHLPPAKFVFADAASNRIGITTDSMVADGCIVSGGTTHR
                     SILFPRVRVNSYARVEESVLMDGVDVGRHARLRRVIVDKGVKIPPGIVIGFDPESDRK
                     RFHVSEGGVVVLPKGVEVPSP"
     gene            2825..3847
                     /locus_tag="PLP50_13575"
     CDS             2825..3847
                     /locus_tag="PLP50_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPA52619.1"
                     /translation="MAMKDPYEVLGVPRTAGDAEVKKAYRRLARKFHPDVNPGDASAS
                     KRFQEIAAAYEILKDPKKKERYDRFGDTGEPPDAASGAPRPGAWGAGPFGDASRTSPF
                     GRGGGAGGGFRWSGDFSDLFSDLFSGTGGGIGVGEDEDAAANLTIPFRDAVLGGTVSF
                     SARIPRRCARCRGTGRAAKAVCPACHGSGALVETESLSVRVPAGVDTGSRIRVAGKGR
                     TENGDLYLSIAVEPHPYFGREGDDIVAEVPVTVPEAYLGAEIEVPTVRGPVRARIPAG
                     TLSGQRFRLKGRGIENLRTGTTGDHYYRVRVIAPTVQSEEGRRLVEALGKLYPRDLRA
                     ELPRGL"
     gene            complement(3966..5066)
                     /locus_tag="PLP50_13580"
     CDS             complement(3966..5066)
                     /locus_tag="PLP50_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52620.1"
                     /translation="MRLPRIAPLALVTAALLGAALLVPAPARADSAAVISPDGTLFEV
                     FPTRYGDVVVVPEGSPDANLQVLALRVTPSGQPGHVELVDGTVDPNAEGSVSIELEAT
                     TRTLFVAYTKANGLMADMHVAVRRDGAWSGQDILSNRGFYLSYNPKVVVTRQSYVDFD
                     EEGLPVTKSRSIFSLVWWEESGPTQARYAPIFVEDGELRVDAVQPWNLNELAGAAGPT
                     DSTGLPLSSYTYPSVQADPSTNGGVIVSFANLATRQQQVLRIAFIDDFVKLSPPGATR
                     VPDEVWARGHFPLGRTAGSGRIPDIMDTGAQVGATISHSGITTFHWVEGGAMNFVRSD
                     DPEGAAARRIALRDDLSAEKALRLVRSLAERD"
     gene            complement(5077..6000)
                     /locus_tag="PLP50_13585"
     CDS             complement(5077..6000)
                     /locus_tag="PLP50_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52621.1"
                     /translation="MTRSTPDAAALRSRSEALRSDAVALLEKGEFAASLGLYDQALDA
                     ARGTGDEAFVDWVFTLRAAAAAESGPADSELIELKRILLRGRDARTAFYASFNAARIY
                     ELRRDFQKAGFYARLATQNARELGEPLLQASAEATTGTLLAVDSRFDEAAAAYGRALE
                     ISAPPAAVPALARSIWQDNLGYCLIALDRTSEGLRLVHDALDSMEALGATAHTVFPLL
                     DLCFGYLKGDRLEEARYFGEACLERAPLRGDETVERNVLYLLGETSHLSGDEDAARDY
                     FDRLASLYPEFRNLRAYLEVFDFRNVINLRS"
     gene            complement(6015..6497)
                     /locus_tag="PLP50_13590"
     CDS             complement(6015..6497)
                     /locus_tag="PLP50_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPA52622.1"
                     /translation="MSTSARRSQIIVVGQKIRQIRKSRRLTQADLASRIGVTQSDLSR
                     MENGEYKVGLDTLFRILQVFELSMSRFFEEPTVETAEAEDAPSPDPATESQQLVADFE
                     ELDENSRREVLDFIAFKKIQQQNRQPRWRPEEAPQEPGPTVAGPLLPFGRPGGPGRAE
                     "
     gene            6582..7349
                     /locus_tag="PLP50_13595"
     CDS             6582..7349
                     /locus_tag="PLP50_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52623.1"
                     /translation="MRRNVFLLSELVRRDLAARYAGSFGGAAWAVLNPLILCGLYTLV
                     FSVIVRVPPPADFRGTYAEFLLAGLLPWLGLQDALVRGAGSVTEQANLVKKIPFPVEI
                     LVLSSLGSALILQMASLVVFAAVSVPFGSGELHLVPLAGAFLFQLLLLAGPVFALAAG
                     SVLFRDLAQLLGPLLTVAFYLTPILYPEDLVPASLAPALTFNPLRDLAGLFRAGLLGT
                     AAPPPGRLLAWSAGLAVLAFLGHRFFARSRRAFADVL"
     gene            7420..9168
                     /locus_tag="PLP50_13600"
     CDS             7420..9168
                     /locus_tag="PLP50_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPA52624.1"
                     /translation="MKRLALVLPLALLAWTGCSSTPRSDAPAPAPEASADAAKEAAAR
                     EALAERERKTVEAARMLAGLPPVSEEDQSERQEWIIVTDPATGKKLQRIPKNPSLRVV
                     DGKLRHTQLNPRMFTLDLVKEDEGFYYVAAPEEVTERPAVDREAEAEGLRPIVEIDPL
                     EYEVVTPAPSKVRLRLEEKSEGLPTSGFWRSNMAVADLDGDGRPEIVTTPPRLGMGTI
                     EIFRYDGARWGSVEPVIEGDETLRYAYGGVAVADMDGDGRPDVVAIGHGSGPFVSYNL
                     GGFRFRREERGLPRQMSGRAVSAGDLDGDGRVDLVAISDESEGSNLRRLQAKAEAEGR
                     PAPGTSEGGYKLGIDTRAFFGGADGRFVEKSAGLEVSCFGYTLELNAAPRDAGAPFFA
                     SGCRYQGGRAILFEYDRQAGAFFSKGRGLAEDYAFHSGTALGTYQGKPAAFVTYVKGN
                     ARGATKGPIRGHGLSIYYRDGQEWKRKRLLKVLANVGTESQGIGVGDLNGDGLDDVAL
                     ADDAVGRVRVFFQTPQGEFEELDPALQPKHVNRSMSVKITDVDGDGKNDVVLMYEYRT
                     AAKTRAGGIRFFRNAG"
     gene            9453..>10887
                     /locus_tag="PLP50_13605"
     CDS             9453..>10887
                     /locus_tag="PLP50_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52625.1"
                     /translation="MKKFTVLVALALLAATGTSYAVTCAYDNVPAATLLVPYFKVSRN
                     GSTGADIPEGGVDTLVAITNVSEWGVVAHVTVWNKQSAAVLDFNIPMTGYDVATFGMR
                     AILNGKLNVNPNVMKPGAVYPDPCDNPATIGFTQTTFQRFRNPSAADEADTLGYYATP
                     AFSGSFRSRVWTSLDEGADYAGLVDPQPTDVYNPACGDVTGSASLAGDFTGYLTIDVT
                     NYCTNFFPDEAQFYVNDAIATLGWDNNDGPNVLMGDVFFVDPAANAGNISGEVAVPLE
                     FDNRLDWTTDRTFYNRYTVDSRSGAITDPGTAIAGVPPAFAYVGDGREPLGVAYGYRY
                     MNASEFDAASFLTVWRSDLWRDGAIDEDGFSTLCAGLTARNYEPVGIFTWDEDENYNT
                     VQGGGPSGDVAPSVDPYIWWESQRLVLADNADVNPGGFKFGWTRVDFPYNEGPRFHQA
                     YVGIQHSAAGQFVSVGHAATMLDNDFTC"
BASE COUNT         1667 a         3699 c         3775 g         1746 t
ORIGIN      
        1 ccctctatgc tgcggccgga tgaacgccca gagccgttcg cgccggtggc gcgccgcgtc
       61 cctcgtcctc ctcaccgccg cggccctcgc gtccgctccc gctgcttcgg cccagacctt
      121 cgcgatcggc gcgggcggag gcctcctgaa cgacgaaggc tcggccgaaa gcctcaagaa
      181 cttctcgacg ggcgcggggt acggtttcgc cgagatcgcg ctcgacccgg gcctccttct
      241 ccaggcgcgg tacacccgga tgaagctccc gtcgcgggcc gcggacggcc ccgaaatcgc
      301 cgtggatgcg gcgacgctct cggtcgccta cttgttccgc gaggactggt ggcaggccgg
      361 cttcgtcgcc ggaggaggcg gctactggct cggaccgaag gagcccggcc ccggacaggt
      421 ggcggtcgag cagagcgcgt cgcggttcgg gtggcacggc ggcgtgctca cgatcttcgc
      481 gctcggccgg aaggcggacc tgcggctcga ggccgtcggg catctcatcc gggacgacga
      541 ccggcgaaag ccgatcgtcc tgtccgcggc cgtggcctgg aagttctgac cggaatggcg
      601 gggttcccgc ccgacctcgg agggtcgggc gggaagtgcg gcggtgggat tttcctggtg
      661 gggctgatgt gaggggtctg ttttggaggg cccggaggcg gggagccttc ggggcatcgg
      721 gcaggttctg aagatctgac gccacctggc gtggagcgtg ttttccgatc tcctgccact
      781 ctatacgcaa tcgaaatgcc accggatttc ccggacgtgt cccgagccgg aaccgcctga
      841 gaagcgggtt ttcggacgcg ccagtcgcgg agtcggagag cgccgtctca aatcgatccc
      901 ggacgtgacg catttcggca tccgccgcga ccgaaatgac acccggagcg agactcgggg
      961 ccggctcagg cgacgggcag gagggtggag tccttcttga tggacagcga gaggtcgacc
     1021 cgcgttcggg cgacgccgcg aatcgtctgg atccgctcgc gaaggagggc ttcgagctgc
     1081 tccaggtcgc gcgccctcac cttgagtcga tagccgtagt ccccggagat cgaatagcac
     1141 tcctggaccg ggatcaggtt ctcgatctcg tccaggaacg ccctctcgaa gctcggatgc
     1201 tcgagctgga cgtcgacgtg cgcgaccgcg gccattccga cctgccgcgg gtcgaggagc
     1261 gcggcgaatc gccggatgaa gcccttcgtc tcgagcttct tgatccgctc gttcaccgac
     1321 ggagtcgtca ggcccacact ctcggcgatc cgcgcctggg agaccttccc gtcgcgctgg
     1381 agcatgtcga ggatctggcg gtcgatctca tccataaaat attcggagag gggccgaaac
     1441 ctccgaactt tatatggagg agcgcctgcg aatgcaagct ctttcgttca gggcgagggg
     1501 acttcgacgc ccttcgggag gacgacgacg cccccttcgg agacgtggaa gcgcttccga
     1561 tcgctctccg ggtcgaagcc gatgacgatc ccgggcggga tcttcacccc tttgtccacg
     1621 atgacccgcc ggaggcgggc gtgccgcccg acgtcgacgc cgtccatcag gacgctctcc
     1681 tctacccgcg catacgagtt gaccctcacc cgcgggaaga ggatcgaccg gtgcgtcgtc
     1741 ccgccggaga cgatgcagcc gtcggccacc atcgagtcgg tcgtgatccc gatccggttg
     1801 gacgccgcgt cggcgaagac gaacttcgcc ggagggaggt gcggagagct gctcttcagg
     1861 gaccagcggg ggttgtagag gttcagcggg gggctgacgg agatgaggtc catcgacgcc
     1921 tcccagtagg agtcgacggt cccgatgtcg cgccagtacg gcgcgagctc ctcgtcgccc
     1981 gggacgcggc tgagagagaa gtcgtaggcg aagacggacg ccgcgccgtc gagcagggcc
     2041 gggatgatcg tccggccgaa gtcgtgcgac gtgtcctgaa gggcgtcgtt ctccagcgcc
     2101 tcgcggagga ccttcggccg gaagatgtag ttccccatcg aggcgagcgc gaagccgggc
     2161 gctccgggga tctcgcgggg ctcccgcggc ttctcctcga agccggtcac ccggccgcgg
     2221 tcgtcgacct cgagcactcc gaagcgggtc gcctccgcga cgggcaccgg gaacgccgcg
     2281 atcgtcaggt cgtggtcgcc gtcctcgtgg aacgcgagca tcgggtttac gtccatgacg
     2341 tagacgtggt cgccgccgaa gacgcagacc tgggacggct cctcgtcgaa gatcaggtcg
     2401 aggttctggt agacggcgtc ggccgtcccg cggaaccagt gtggtccgcg ccgcatctgc
     2461 gccgggacgg gatcgacgaa ctgcccgatc tcggggttca gcctccaggc cctcacgagg
     2521 tgctcgatca gcgagttcga cttgaattgc gtcaggacct tgatcttcat caggcccgag
     2581 ctgacgaagt tgttgaggac gaagtcgacg atccggtagc ggcccccgaa cggaacggcg
     2641 ggcttggcgc ggtcacgggt cagcgggtag aggcgctcgc ccatcccgcc ggcgaggacg
     2701 atcgagagga cgtccctgtg ctggaggatc ttcacggcgg agcacctcgg tcgactccat
     2761 tctaggagcc tcggctccga aggctccctg cgcggccgtt cccgtttctt ctatccttct
     2821 ccgtatggcg atgaaggacc cctacgaggt gctcggcgtc ccgcgtacgg ccggggacgc
     2881 cgaggtcaag aaggcctacc ggcgcctcgc tcggaagttc catcccgacg tgaatcccgg
     2941 cgacgcctcc gcgagcaagc ggttccagga gatcgccgcg gcctacgaga tcctgaaaga
     3001 cccgaagaag aaggaacgct acgaccggtt cggggacacc ggcgaaccgc cggacgccgc
     3061 ctccggggcg ccccgcccgg gggcctgggg ggctggcccg ttcggagacg cgtcgcgaac
     3121 ctcgccgttc ggcagaggcg gaggagctgg cggaggcttc cgctggtcgg gcgacttctc
     3181 ggacctcttc tcggatctct tctcgggcac cgggggcggg atcggcgtcg gcgaggacga
     3241 ggacgccgcc gcgaacctga cgatcccgtt ccgcgacgcc gtcctcggcg ggaccgtttc
     3301 cttttcggcg cgcatcccgc gccggtgcgc acgctgccgg gggacgggcc gcgcggcgaa
     3361 ggccgtctgc ccggcctgtc acggctcggg cgccctcgtc gagaccgaga gtctcagtgt
     3421 ccgggtcccc gcgggcgtcg ataccggatc gcggatccgc gtcgccggga aggggaggac
     3481 ggagaacggc gacctctacc tctccatcgc cgtcgagccc catccgtact tcgggcggga
     3541 aggcgacgac atcgtcgcgg aggtgccggt aacggtgccg gaggcctacc tcggcgccga
     3601 gatcgaggtc ccgacggtgc gcggaccggt ccgcgcccgg atccccgccg ggaccctctc
     3661 gggacagcgc ttccgcctga aggggcgcgg catcgagaac cttcgcacgg ggacgacggg
     3721 cgaccactac taccgggtcc gggtgatcgc cccgacggtc cagagcgagg agggacgccg
     3781 gctcgtcgag gcgctcggga agctctaccc gcgggacctc agggccgagc tgccgcgcgg
     3841 gctctgaggt ccggggtcgt cgcgttccac ctcgcgcacg gcacgacggc gcgcggccct
     3901 tccccaagag tagaagcgcc ccggcgggcc ggggcgcttc ctcgtgacgg tccgtcgttc
     3961 gggtgtcagt cccgctcggc gagcgaccgg acgagccgga gcgccttctc cgcggagagg
     4021 tcgtcgcgga gcgcgatccg gcgcgcggcg gcaccctcgg ggtcgtcgct gcggacgaag
     4081 ttcatcgcgc cgccctcgac ccagtggaac gtcgtgatgc cggagtggga gatcgtcgcg
     4141 ccgacctgcg cgcccgtgtc catgatgtcg ggaatgcgcc cggagcccgc ggtccggccg
     4201 agcgggaaat ggcctctcgc ccagacctcg tccgggacgc gggtcgcgcc gggagggctg
     4261 agcttgacga agtcgtcgat gaacgcgatc cggaggacct gctgctgacg ggtcgccagg
     4321 ttcgcgaacg agacgatgac gccaccgttc gtcgaggggt ccgcctggac ggacgggtac
     4381 gtgtacgaag agagcgggag gccggtcgag tcggtcggcc cggcggctcc ggcgagctcg
     4441 ttgaggttcc agggctggac agcgtcgacg cggagctctc cgtcctcgac gaagatcggc
     4501 gcgtagcggg cctgcgtcgg gccgctctcc tcccaccaga cgagggagaa gatcgagcgg
     4561 ctcttcgtga cgggcagccc ttcctcgtcg aagtcgacgt acgactggcg cgtcacgacg
     4621 accttcgggt tgtaggagag gtagaaaccg cggttcgaga ggatgtcctg cccggaccag
     4681 gcgccgtcgc gccggacggc gacgtgcatg tcggccatga ggccgttcgc cttcgtgtag
     4741 gccacgaaga gggtccgggt cgtcgcctcg agctcgatcg agaccgagcc ctcggcgttc
     4801 gggtcgaccg taccatcgac gagctcgacg tgacccggct gaccggacgg cgtcacgcgc
     4861 agggcgagca cctggaggtt ggcgtcgggc gagccttccg ggacgacgac gacgtcgccg
     4921 tagcgggtgg ggaagacctc gaagagcgtc ccgtcgggcg agatgaccgc cgccgagtcg
     4981 gcccgcgcgg gtgcgggcac gagcagcgcg gctccgagga gagccgcggt gacgagggcg
     5041 agaggggcga tacggggcaa tctcatggcg ctcacctcac gacctgaggt tgatgacgtt
     5101 ccggaagtcg aagacctcga gataggccct gaggttccgg aactcgggat agagggacgc
     5161 gagccgatcg aagtagtcgc gggcggcatc ttcgtcgccg ctgaggtggc tcgtttcccc
     5221 gagcaggtaa aggacgtttc tctcgactgt ctcgtctccg cgaagagggg cccgctcgag
     5281 acaagcttcc ccgaaatagc gggcctcttc gaggcggtca cctttcagat acccgaagca
     5341 gaggtcgagg agaggaaaga cggtgtgagc ggtcgctccg agcgcctcca tggagtcgag
     5401 cgcgtcgtga accagtcgca gcccttccga ggtacggtcg agggcgatca ggcagtatcc
     5461 gaggttgtcc tgccagatcg agcgggcgag cgcgggaacg gcggccggag gggcggagat
     5521 ctccagggcg cgaccgtagg ccgccgccgc ctcgtcgaat cgactgtcga cggcgaggag
     5581 cgtcccggtc gtcgcctcgg cgctggcctg aaggaggggc tcgcccagct cgcgggcatt
     5641 ctgcgtcgcg aggcgtgcgt agaaccctgc cttctggaag tcgcgcctga gctcgtagat
     5701 tcgagcggcg ttgaacgagg cgtagaaagc ggttcgcgcg tcgcgcccac ggagaagaat
     5761 gcgcttgagc tcgatcagct cgctgtcggc tggtccggat tccgcggcgg cggcggcacg
     5821 cagggtgaaa acccaatcga cgaatgcctc gtcgcccgta ccgcgagcgg cgtcgagcgc
     5881 ctggtcgtac aggcccagcg aagccgcgaa ctcgcccttc tcgaggaggg cgaccgcgtc
     5941 cgaccgcagc gcctcggaac ggctgcggag cgctgcggcg tccggtgtcg atctcgtcat
     6001 ctcgcttctc gacctcactc ggcgcgaccg gggccgccgg ggcgcccgaa cgggagaagg
     6061 ggacccgcga cggtcggccc gggctcctgc ggagcctcct cgggccgcca ccggggctgg
     6121 cggttctgct gctggatctt cttgaacgcg atgaagtcga ggacctcgcg gcgcgagttc
     6181 tcgtcgagct cctcgaagtc ggccacgagc tgctgcgact ccgtggccgg atcgggcgac
     6241 ggcgcgtctt ccgcctccgc cgtctcgacc gtcggctcct cgaagaagcg gctcatcgag
     6301 agctcgaaga cctggaggat ccggaagagc gtgtcgaggc cgaccttgta ctcgccgttc
     6361 tccatgcgcg agaggtcgga ctgggtgacg ccgatgcggc tcgcgaggtc ggcctgggtc
     6421 aggcggcggc tcttccgtat ttgacggatt ttctgcccca ccacgataat ctgcgaccgg
     6481 cgcgcgctgg tactcatcga cacgaacccg aacctaaggt taagccctcc cgggtccctg
     6541 tcaagcggtt cgtcccgtga cgagcgcccg ccggaccaga tttgagacga aacgtcttcc
     6601 tcctctccga gctcgtccgg cgagatctgg ccgcccgcta cgccggaagc ttcggcggcg
     6661 cggcctgggc ggtcctgaac ccgctcatcc tctgcggcct ctacacgctc gtcttttccg
     6721 tcatcgtccg ggtccctcct ccggccgatt tccgcgggac ttacgccgag ttcctcctgg
     6781 ccgggctcct gccctggctc gggctccagg acgcgctcgt acgaggcgcc ggctcggtga
     6841 cggagcaggc gaacctcgtg aagaagatcc cgttccccgt cgagatcctc gtcctttcga
     6901 gcctcggctc ggcgctgatc ctccagatgg cgagcctggt cgtcttcgcg gctgtctcgg
     6961 ttccgttcgg ctccggcgag ctgcatctcg ttccgctggc cggggcattc cttttccagc
     7021 ttctcttgct cgctggccct gtatttgctt tagcggcggg cagcgtgctg tttcgggacc
     7081 tcgcccagct cctcggtcca ctcctgaccg tggccttcta cctgacgccg atcctctatc
     7141 cggaggacct cgtcccggcg tctctggcgc cggcgctgac gttcaacccg ctgcgcgacc
     7201 tcgcggggct cttccgcgcc ggccttctcg gcacggcggc gccgcccccc gggcggctcc
     7261 tcgcctggag cgccggcctc gccgtgctag catttctcgg tcaccgattc ttcgcgcgga
     7321 gccgcagggc tttcgccgat gtcctctgat catcgggagc ttcctcccat cgagccgcga
     7381 gccgaaggtt cgcggctctc gtgcgcccca ggagacgcct tgaagagact cgccctcgta
     7441 cttcccctcg ccctgctcgc atggacgggc tgctcttcca ccccccgttc cgacgccccc
     7501 gcgccggctc cggaggcgtc ggccgatgcc gcgaaggaag cggccgcccg tgaggcgctc
     7561 gccgagcggg agcgcaagac cgtcgaggcg gcgcgcatgc tcgcgggcct cccgcccgtg
     7621 tccgaggagg accagtcgga gcgccaggag tggatcatcg tcaccgaccc ggcgacgggg
     7681 aagaagctgc agcggatccc gaagaacccg tcgctccgcg tcgtcgacgg aaagctccgt
     7741 cacacccagc tcaacccccg gatgttcacg ctcgacctcg tgaaggagga cgaagggttc
     7801 tactacgtcg cagcgccgga ggaggtcacc gagaggcccg cggtcgacag ggaggcggag
     7861 gccgaagggc tccgcccgat cgtcgagatc gaccctctcg agtacgaggt cgtcaccccg
     7921 gcgccttcga aggtccggct ccgtctcgag gagaagtccg aagggcttcc cacgagcggc
     7981 ttctggcggt cgaacatggc ggttgccgac ctcgacggtg acgggcggcc cgagatcgtc
     8041 acgaccccgc cgcggctcgg catgggaacg atcgagatct tccgctacga cggtgcgcgc
     8101 tggggctccg tcgagccggt catcgagggc gacgagacgc tgcgctacgc ctacggcgga
     8161 gtcgccgtcg cggacatgga cggcgacggc cgacccgacg tcgtcgcgat cggccacggc
     8221 tcgggaccgt tcgtctccta caacctcggc ggcttccggt tccgacggga ggagcgcggg
     8281 ctaccgcgcc agatgagcgg gcgggcggtg tcggccggag acctcgacgg cgacggccgc
     8341 gtcgacctcg tcgcgatctc ggacgaatcg gagggctcga acctccgacg cctccaggcg
     8401 aaggcggaag ccgaggggcg cccggccccg gggacctcgg agggcggcta caagctcggg
     8461 atcgacacgc gcgccttctt cggcggcgct gacgggagat tcgtggagaa gtcggcgggg
     8521 ctcgaggtct cctgcttcgg ctacacgctc gagctgaacg ccgccccgcg cgacgcgggg
     8581 gcgccgttct tcgcgtccgg ctgccggtac cagggcggtc gggcgatcct cttcgagtac
     8641 gaccggcagg ccggcgcctt cttcagcaag ggccgcgggc tcgccgagga ctacgcgttc
     8701 cattccggga ccgcgctcgg cacctaccag gggaagccgg ccgcgttcgt cacctacgtc
     8761 aaggggaacg cccgcggcgc gacgaaagga ccgatccgcg gccacggcct ctccatctac
     8821 taccgagacg gccaggagtg gaagcgaaag cgcctcctca aggtcctcgc gaacgtcggg
     8881 accgagagcc aggggatcgg ggtcggcgac ctgaacgggg acggcctcga cgacgtggcc
     8941 ctcgcggacg acgccgtggg ccgggtccgg gtcttcttcc agacgccgca gggggagttc
     9001 gaggagctcg acccggcgct ccagccgaag cacgtgaatc gttcgatgag cgtgaagatc
     9061 accgacgtgg acggcgacgg gaagaacgac gtcgtcctca tgtacgagta tcggactgcg
     9121 gcgaagacgc gcgccggagg gatccggttc ttccggaacg ccggatgacc ggagcgccgg
     9181 gccggtccac cgctcgtccg tatgggatgg cagcatattt ttcgttcccg acgcttgaca
     9241 tttccgaata gcgcccctag tcttcccctt agcgccatga gtctgacttt ccttttccgt
     9301 gttccggaat gccccgaagg ggcggtcgga tgcggagtag cattcgggaa tcccatcgaa
     9361 ttcacgggag gtgattcggt atcgggggag ttcgtagctc tcagtcgtca ggtttcagcc
     9421 ccatcgatca cactgaatca gcaggagtaa agatgaagaa gttcaccgtt ctggttgccc
     9481 tggcgctcct cgcggcgacg gggacgagct acgccgtgac ctgcgcgtat gacaacgtgc
     9541 cggccgcgac gctcctcgtg ccgtacttca aggtgtcgcg caacggctcc acgggcgccg
     9601 acatccccga gggcggcgtc gacaccctgg tcgccatcac caacgtttcc gagtggggcg
     9661 tcgtcgccca cgtcaccgtc tggaacaagc agtcggcggc ggtcctcgac ttcaacatcc
     9721 ccatgacggg ctatgacgtc gccaccttcg gcatgcgcgc catcctcaat ggcaagctga
     9781 acgtcaaccc gaacgtgatg aagccgggcg ccgtgtaccc cgatccgtgc gacaaccccg
     9841 ccacgatcgg cttcacgcag accacgttcc agcgcttccg taacccgtcc gcggcggacg
     9901 aggccgacac gctcggctac tacgccaccc cggccttcag cggctccttc cgcagccgtg
     9961 tctggacgtc tctcgacgag ggcgccgact acgcgggtct cgttgatcct cagccgaccg
    10021 acgtctacaa cccggcctgc ggcgacgtga cgggctccgc gagcctcgcc ggcgacttca
    10081 ccggctacct cacgatcgac gtgacgaact actgcacgaa cttcttcccg gatgaagcgc
    10141 agttctacgt caacgacgcg atcgcgacgc tcggctggga caacaacgac ggcccgaacg
    10201 tcctcatggg cgacgtcttc ttcgtcgacc cggccgccaa cgccggcaac atttccggcg
    10261 aggtcgccgt cccgctcgag ttcgacaacc gcctcgactg gacgacggac cgcaccttct
    10321 acaaccgcta cacggtcgac agccgctccg gcgcgatcac ggatccgggc acggcgatcg
    10381 ccggcgtccc gcctgccttc gcctacgtcg gcgacggccg cgagccgctc ggcgtggcct
    10441 acggctaccg ctacatgaac gcctccgagt tcgatgccgc cagcttcctc acggtgtggc
    10501 gctccgacct gtggcgtgac ggcgcgatcg acgaggacgg gttctcgacg ctctgcgcgg
    10561 gcctcacggc gcggaactac gagccggtcg gcatcttcac gtgggacgag gacgagaact
    10621 acaacacggt tcagggtggc gggccttcgg gcgacgtcgc tccgtccgtc gacccgtaca
    10681 tctggtggga gtcccagcgc ctcgtcctcg cggacaacgc cgacgtgaac cccggcggct
    10741 tcaagttcgg ctggacgcgc gttgacttcc cctacaacga gggcccccgc ttccaccagg
    10801 cctacgtcgg catccagcac tccgcggctg gccagttcgt cagcgtcggc cacgcggcca
    10861 cgatgctcga caacgacttc acctgcg
//
LOCUS       DAOONL010000113        10632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7063_length_10632_cov_18.741326, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000113 DAOONL010000000
VERSION     DAOONL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_10632_cov_18.741326"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..683
                     /locus_tag="PLP50_13610"
     CDS             459..683
                     /locus_tag="PLP50_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52626.1"
                     /translation="MSPTVVAGGGGDHLVGWAGDCHLPGTPSLAFAPGRARENHGVIE
                     LIRDGTRTHRVKSTAASSVDDILDVTGHFE"
     gene            complement(664..1650)
                     /locus_tag="PLP50_13615"
     CDS             complement(664..1650)
                     /locus_tag="PLP50_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52627.1"
                     /translation="MRSLLNLFTLITATTLLAAPGRGPRLLAPGVVATGESESHATLS
                     PDGGTLYFVKLTPDFAHWTAVASERRAERWTEPSVAWFSGRWSDADVSFAPDASTLYF
                     ISNRPDRASDPARADHDIFRMRRTSAGWSEPERIVELSSPGAEWFPNQTSDGTLYFGS
                     ERQAGNLGPAGTSDLWRARWLGDRFAEPENLGPTINTAGQDIEPWISADGRTLVFASK
                     GRADSLGSYDLYVSRACSGVWSEPRSLGGGVNSPAWEFGGRFSPDGRTFFFASNRTIA
                     SPADRTLAGRGGYRELVGRLRSPGNGLFDVYEIDASELGLGAPCDAPTRSGR"
     gene            complement(1858..2148)
                     /locus_tag="PLP50_13620"
     CDS             complement(1858..2148)
                     /locus_tag="PLP50_13620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPA52628.1"
                     /translation="MLLSAGLFAAIHGINPESYWFAFVYAFLLGVLLAEIVLTRGDLG
                     CVIGFNFAWNLLQDKGLLNLPERGGEGAYMAVLLIGLVLTNWKIPPRTTGNG"
     gene            complement(2415..3575)
                     /locus_tag="PLP50_13625"
     CDS             complement(2415..3575)
                     /locus_tag="PLP50_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA52629.1"
                     /translation="MELARLHVTVVGGATGGAAAALLLARAGARVTLLERALHPRALG
                     AGIGLADNGLAVLESLGLLPALAERARPVGAARIVDGARRTIYSPPGAPPRVWMVRRG
                     DLQGLLLDALAAEPRIDVRFGAAVERATPDGRVRLRAAGRGEELAADLVIGADGVDST
                     VRESGGFGARVPPPGIPYLRTLVEGAEALGEEAWTAAGLFGSFPVPGGVYVYASAGSR
                     ATRSAVAARDLAALRSAWAGAYPASASLLAAIRRFDDLLVHPVRRIDCARWFDGRLAL
                     LGDAAHAMAPNLGQGANSALVDAAVLLDELRQAGTLPAALAAFEARRRPAVRRVADAA
                     ARLGALAEWTHPLARALRDRALMPLAARLSRASDLARVLQEPRETLLAIGRA"
     gene            3678..4688
                     /locus_tag="PLP50_13630"
     CDS             3678..4688
                     /locus_tag="PLP50_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HPA52630.1"
                     /translation="MQRTSVLAALTRGLLDAARAIGRDPDALAAAAGLDPAWLAGPDQ
                     RVPLANHFRLWVELSRRPDGLALGEALGLSGLGVVGYAMQHGATVGEALEWQQRYGAV
                     VHPDVNPRLERRREVAGERLVFVQVVPAPFVELREPVDAQAAAIVTALAGLAGRALRA
                     RWVAFPRARPADAERHESWFSCPVAWGAPALEVAFEAAVLDLPLPRSDARLFGYLARR
                     AESLHAALPAATSFAERARREIGDALARGEPRAAEVARRLAVSERTLSRRIAEEGTGF
                     AQLVDAARRERALLLLEDPSLSASEIAFLLGYAEPAVFFRVFKRWTGETPGGWRRRRS
                     ET"
     gene            5193..6209
                     /locus_tag="PLP50_13635"
     CDS             5193..6209
                     /locus_tag="PLP50_13635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPA52631.1"
                     /translation="MRTRPPALALFLLLTFVPAWALWIGSGVLSRAGKQPADAAWLMA
                     QLGVFSPALAAFVVASLERPDARKTTVRLAGFLYLPALVLGAVITTRGFDDLRRVGAV
                     WLLPVGLLALASLVLLGRGDGRVEPWPLGGAGRGVVALWTAGAALLPVAVYGAAWAAG
                     GAAPPAAGVPAAAPYRDLTAATALLALGWNLVFGGSLGEEPGWRGFLLPRLLADRTPF
                     GASLVVGFWWGAWHAPIDWVQGFVVSGPGALVARQVWTLPLAVLFAWVTVRAGGSLLP
                     AIACHTALNAVPDFGLVDPARYASATAGFWAAALVAAVAVMALDPVLLRLPGARPAES
                     GPRT"
     gene            6281..6799
                     /locus_tag="PLP50_13640"
     CDS             6281..6799
                     /locus_tag="PLP50_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52632.1"
                     /translation="MRTIFFVPVGAMAVAAALSLFGPGLPAGPAPAGETRLERAARVI
                     ADESRTPEAVGAALRDLLDEAIAAAPASGLAGEWPAKVARARELLAVRPPAHDEARRL
                     LAESWREAFGTAWAFPAAVATIDDATALAERNVGAAATARREGRSGDAVRLLLETVIL
                     VVTPMHAPAARS"
     gene            6909..7442
                     /locus_tag="PLP50_13645"
     CDS             6909..7442
                     /locus_tag="PLP50_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA52633.1"
                     /translation="MSEEDVPDREAEFEELVHRHRDRMIRTVWRVLRDADLAEEALQD
                     ALGTLWRKLDTLRAHPNPEAFVLRVCLDAAYDRLRERVRARRRDAPEDEAAPGPGGSG
                     DRVEVREVLEAIRRLDRRQATAILLHAVQGEPYRAVAEALGCGEATARVHVQRAREKL
                     RRAFGRSVARPGKETEP"
     gene            7439..8083
                     /locus_tag="PLP50_13650"
     CDS             7439..8083
                     /locus_tag="PLP50_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52634.1"
                     /translation="MNEWRSDDTFGARDVDARLSSALRDDVPPEVARRLERRAAVEVA
                     RRRIRRPFLLALLDRLVPAPALTRLAIPAGLVLLASGALLQGSAWPSEAREALQRLNV
                     TASASRALGEAGPLLCGAGSPLEGLGPEALSDRAFRSWLLVGTEEVPEGRLRLVFRAP
                     RERARYEVVVDAASFRPRTIRRTRLDAPPGGVPVSDECAWPGNDGRPVPERRSR"
     gene            8080..8910
                     /locus_tag="PLP50_13655"
     CDS             8080..8910
                     /locus_tag="PLP50_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52635.1"
                     /translation="MNVSAPIAGSVFLFLVLLALDGPEWFRRDTTDAGARLRRLAFDA
                     WAAGVLASHLFFVGALGRPDRGLAATAREMTLAFLPALLGLGVAAAAGMRAFAPSGRD
                     AAPDAGEVRAAAPLASAAAFVVLVAAGLLAATHTGGEAQLAPVAVLLHPPALLAFAGA
                     ALLLLRLAGRGATRNALAPAIALAALLAGLAGLFQALSGFAARDLGRVTAGLAFLLTS
                     GFAGLLGLLLASRAPGAGGRRGGASVAGGVARALLPLAALLFLAFAFVAVLTPMKAPG
                     "
     gene            complement(8938..9414)
                     /locus_tag="PLP50_13660"
                     /pseudo
     CDS             complement(8938..9414)
                     /locus_tag="PLP50_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9577..10524)
                     /locus_tag="PLP50_13665"
     CDS             complement(9577..10524)
                     /locus_tag="PLP50_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA52636.1"
                     /translation="MGRQPGQPHDCAAEYLLAFSCKGRGLCPSCGAKRAAEFAAFLKD
                     EVVADVGHAQWVFTVPKRLRPHFMHHRELLGPLCAAAWQAVRELMAVAAGEEEAFRPG
                     RVAVVQTFGGQLNLHPHVHALVTRGGWTVSGQWVPVPYVSPSAAETLFRHKVIRLLQR
                     EGLLDEDRTRLLLSWRHSGFSVHNSVTVPAGDGRTLEALARYCLRSPVSLTRLRRDPG
                     SQTATYLPRDGHDGGIAETLDALDFVARLLAHVPDPRRHLVHYYGACSNVVRGKLKAR
                     SQARKAEPPASGPAYRPAPPPASRRGLTVEPRLRPHRAG"
BASE COUNT         1282 a         3798 c         4158 g         1394 t
ORIGIN      
        1 gcgacgtaac cattccatgg tagtggcggg actcgtggtg acctttcgcg gcccctgcac
       61 cgggccggtt ccagcaagca gcccggaagg tgggtcgtcg ccctcatgca ccaggggggg
      121 agggcggcat cacgatgcat ccacgtctca gcggaagtcg tggctcgttg gagcgctgtg
      181 gcgctggtgc cggtcgggcg gcgagagggc gcggcgcggc tcggggatcg tctccgcgag
      241 gttcagccgc ccgaagcgat cggcgcggat cgaaagggtg tcgtgcgtct tctgtatgcg
      301 cccctcgacg acgaggaagc gggggagccg acgacgaggg cgcggttctc cgtgaagagg
      361 tcggggcgga ggatcgcctg gcacatctcg atctcgtcct catgcgagag gaagacgaag
      421 cccttcgcgg atccctctcc tgggcacggg ccatcccgat gagcccgacg gtcgtcgccg
      481 gggggggcgg agaccatctc gtcggctggg cgggcgattg ccacctgccc ggcacgccca
      541 gccttgcctt cgcgccaggg cgcgcccgcg agaaccacgg cgtcatcgag ctcattcggg
      601 acgggacgcg gacccaccgg gtgaagagca ccgccgcttc gtccgtagac gacatcctcg
      661 acgtcaccgg ccacttcgag taggggcgtc gcagggcgcg ccgaggccga gctcggaggc
      721 gtcgatctcg tagacgtcga acaggccgtt gccgggcgag cgcaggcgcc cgacgagctc
      781 gcggtagccg ccgcgtcccg caagggtccg gtcggccggt gacgcgatcg tccggttgct
      841 cgcgaagaag aaggtccggc catcgggcga gaagcggccg ccgaactccc aggccggcga
      901 gttgactccg ccgccgagcg agcgcggctc gctccagact ccggagcagg cgcgggagac
      961 gtagaggtcg taggagccga gcgaatccgc gcgccccttc gaggcgaaaa cgagcgtccg
     1021 gccgtcggcc gagatccagg gttcgatgtc ctgtcctgct gtgttgatcg tcgggcccag
     1081 gttctccggc tccgcaaacc ggtcgccgag ccaacgcgcg cgccagaggt cggacgtgcc
     1141 cgccggtccc aggtttcccg cctgccgttc ggagccgaag tagagcgtcc cgtccgacgt
     1201 ctggttgggg aaccactcgg cgcccgggct cgagagctcg acgatccgct ccggctcgga
     1261 ccagccggcg gacgttcgtc gcatccggaa gatgtcgtgg tcggcgcgtg ccggatcgga
     1321 ggcccgatcg gggcggttag agatgaagta gagggtcgac gcgtccggcg cgaacgagac
     1381 gtccgcgtcg ctccagcgtc ccgagaacca cgccaccgac ggctcggtcc agcgctcggc
     1441 ccggcgttcg gaagcgaccg cggtccagtg cgcgaaatcg ggggtcagct tgacgaagta
     1501 gagggtcccg ccgtcggggg atagcgttgc gtggctctcg ctctcgcccg tggcgacgac
     1561 gcccggggca aggaggcggg ggccgcgacc cggcgccgcg aggagcgtcg tcgcggtgat
     1621 cagcgtgaac aggttcagta gagagcgcat gcggcgcggc tcctttcccg atcgactcga
     1681 gggcacccgg ggccggaagg gtcgggtggc cctacctgtg aaccggaaac gggtcaatgc
     1741 cgccgcgcgc cgccgcgcga gggaaacgga ccgccgggga cgatgccgtg caggcgggga
     1801 cggtgaaagg atcccggtgc cgactcgccg ttcaccggcc tgccgggcac gcgcgtccta
     1861 cccgttgcca gtcgtgcggg gcggaatctt ccaattggtc agtacgagac cgatcagcag
     1921 gacggccatg taggcgccct cgcctcctct ttcggggaga ttcaggagcc ctttgtcctg
     1981 gagcagattc cacgcgaagt tgaaaccgat gacgcatccc aggtctcccc gcgtcaggac
     2041 gatctcggcg agcagcacgc cgagcaggaa ggcatacacg aaggcgaacc agtaagactc
     2101 cgggttgatg ccgtggatgg ctgcgaacag acccgcggac agaagcacct cgccattctc
     2161 gagccgcttc ttcccccggg tcctgacggc cgacgccggt caccaccagc ttcgcccacg
     2221 tcgcgctctc ggaaccgacc gccggccagc ccgaggccgc cttcggccga ttttgagggc
     2281 ggcggggcga tcgatcgtcc cggtcggcgt cctggtcaac cacctctacg tcgacggcgc
     2341 cgacctcggc gacctctgcg acgccgcccg cgacagcttc gagcgggcct tccagcttcc
     2401 agcggcgccg ggggtcaggc gcgcccgatc gccagcagcg tctcgcgggg ctcctggagc
     2461 acgcgggcga ggtcgctcgc gcgcgagagg cgggcggcga gcggcatcag ggcgcggtct
     2521 cgcagtgcgc gcgcgagcgg atgggtccac tcggcgaggg cgcccaggcg tgccgcggcg
     2581 tcggcgacgc ggcgcacggc cgggcggcgc cgcgcttcga aggcggccaa cgcggcgggc
     2641 agcgtcccgg cctggcgcag ctcgtcgagc agcacggccg cgtcgaccag cgcgctgttg
     2701 gcgccctggc cgaggttcgg cgccatcgcg tgggcggcgt cgccgagcag cgcgagtcgg
     2761 ccgtcgaacc agcgcgcgca gtcgatcctc cgcaccgggt ggaccagcag gtcgtcgaag
     2821 cgccggatcg ccgcgagcaa cgaagcggag gcggggtagg ctccggccca cgccgagcgc
     2881 agcgcagcga gatcgcgcgc ggcgaccgcg gagcgcgtgg ccctcgagcc ggcggaagcg
     2941 tagacgtaga cgccgcccgg cacagggaag ctgccgaaca ggccggccgc ggtccaggcc
     3001 tcctcgccga gcgcctctgc cccttcgacc agcgtgcgca gatagggaat gcccggcgga
     3061 ggcacgcgcg cgccgaagcc gccgctctcg cgcactgtgg aatcgacccc gtcggccccg
     3121 atcacgaggt cggcggcgag ctcctcgccg cggcccgcgg cgcggaggcg gacgcggccg
     3181 tcgggcgtcg cgcgctcgac ggccgcgccg aagcgtacgt cgatccgggg ctcggccgcg
     3241 agcgcgtcga ggagcaggcc ctgcaggtcg ccgcggcgga ccatccagac ccgcggcggc
     3301 gcgccggggg gcgagtagat cgtccgccgc gcgccgtcga cgatgcgcgc cgcgcccacc
     3361 ggccgcgccc gttcggcgag cgcgggcagc agtccgagcg actcgagcac ggcgagcccg
     3421 ttgtcggcca gcccgatgcc ggcgccgagg gcgcgggggt ggagcgcccg ctcgagcagg
     3481 gtcacgcgcg ccccggcccg tgcgagcagc agcgccgccg ccgcgccccc cgtcgcgccc
     3541 cccaccaccg tcacgtgcag tctcgcgagc tccatggtct cctccccagg gaagatcctg
     3601 gccgaggcgc ggcggcgcga cgatgatcct cgctgccggt cttgtgatcc tggccgccca
     3661 tcgcggatag ttgcgacgtg caacggacct cggtgctggc ggcgctgacg cgcggactgc
     3721 tcgacgcggc gcgggcgatc gggcgcgatc cggacgcgct cgccgcggcc gccggcctcg
     3781 acccggcctg gctcgccgga cccgaccaga gggtgccgct ggcgaaccac ttccggctct
     3841 gggtcgagct gtcgcggcgc cccgacggtc tcgcgctggg cgaggcgctc ggcctctccg
     3901 ggctcggcgt cgtcggctac gcgatgcagc acggcgccac ggtgggcgag gcgctcgagt
     3961 ggcagcagcg ctacggcgcg gtggtccacc ccgacgtgaa cccgcggctc gagcggcgcc
     4021 gcgaggtggc cggcgagcgg ttggtcttcg tgcaggttgt gccggcgccg ttcgtcgagc
     4081 tgcgcgagcc ggtcgacgcg caggcggcgg cgatcgtgac cgcgctcgcg ggcctcgcgg
     4141 gacgcgctct gcgcgcccgc tgggtcgcct tcccgagggc tcgcccggcg gacgcggagc
     4201 gccacgagag ctggttctcc tgcccggtgg cctggggcgc tccggcgctc gaggtcgcct
     4261 tcgaggccgc ggtcctcgac ctgccgctgc cgcgctccga cgcgcgcctg ttcggctacc
     4321 tcgcccgccg cgccgagtcg ctccacgccg cgctccccgc cgcgacgagc ttcgccgagc
     4381 gggcgcgacg ggagatcggc gacgcgctgg cgcgcggcga gccgcgcgcg gcggaggtcg
     4441 cccggcggct ggccgtctcc gagcgcacgt tgtcgcgtcg catcgccgag gagggcaccg
     4501 gcttcgcgca gctcgtcgac gccgcgcgcc gcgagcgcgc gctgctcctg ctcgaggatc
     4561 cctcgctctc cgcctcggag atcgccttcc tgctgggcta cgccgagccc gcggtcttct
     4621 tccgcgtgtt caagcggtgg accggggaga cgcccggcgg atggcggcgc cggcgatctg
     4681 aaacctagcg ggagtcggcg ttctcggtcg agggcggctc ggcggccaag gccagggcgg
     4741 tcgggtcccg cgctcggctc gggtgcgcga ggtgttcctc acgtcggcgg aggataggaa
     4801 gtcggatttt cggcgacggc gaggcagagg gtcggaggcg acgctggccg gcagggcaac
     4861 aggggagcac gggagctggc agggcgacgg gagggggagc gcggccctga gaaggggtgg
     4921 gagccggggc ggcgaggtgg tcgaggatgc ggcggatgac gcggggctcg gtgatgaagg
     4981 cgacgacccg catcacgccc tggcagcggg gacagacgag cggatcgact tcgtacacct
     5041 ggcgaatgag ctgagcccat ccccggcgaa gggcggccag ggatggggac accggggcga
     5101 gcggcagtcc ggggagcaac tacgatacct ccctcgcgcg ccgccggccg ggccggccgg
     5161 cgcctgcggg gcgggcaggg gaggccgcca tcgtgaggac ccgacccccg gcgctcgcgc
     5221 tcttcctcct cctcaccttc gtcccggcct gggccctctg gatcgggtcg ggcgtcctct
     5281 cgcgggcggg gaagcagcct gcggacgccg catggctgat ggcgcagctc ggcgtcttct
     5341 cgcccgccct cgccgccttc gtcgtcgcct cgctcgagcg gccggacgcg aggaagacga
     5401 cggtccggct cgccgggttc ctctacctgc cggccctcgt gctcggggcc gtgatcacga
     5461 cccgcgggtt cgacgacctc cgccgcgtcg gcgcggtctg gctcctcccg gtcgggctcc
     5521 tcgccctcgc gtcgctcgtc ctcctcggcc ggggggacgg ccgcgtggag ccgtggccgc
     5581 tcggaggcgc cgggaggggc gtggtcgcgc tctggacggc gggcgccgcc ctcctgccgg
     5641 tcgcggtcta cggagcggcg tgggccgccg gtggagcggc gccgccggcc gccggcgtgc
     5701 cggccgcggc gccgtatcgc gacctgaccg ccgcgaccgc gctccttgcc ctcggctgga
     5761 acctcgtctt cggcgggagc ctcggcgagg agcccggctg gcgcggcttc ctgctgcccc
     5821 ggctcctcgc cgaccgcacg cccttcgggg cgagcctcgt cgtcggcttc tggtgggggg
     5881 cctggcacgc gccgatcgac tgggtccagg gcttcgtcgt gagcggcccc ggcgccctcg
     5941 tggcccggca ggtctggacc ctgcccctcg ccgtcctctt cgcctgggtg acggtccggg
     6001 ccggggggag cctcctcccg gcgatcgcct gccacaccgc gctcaacgcg gtccccgact
     6061 tcggcctcgt cgacccggcg cgctacgcct ccgcgacggc cggtttctgg gccgcggcgc
     6121 tcgtcgccgc cgttgccgtc atggcgctcg acccggtcct gctccgcttg ccgggcgcgc
     6181 ggccggcgga atccggcccg cggacgtaaa cgaatcgacg cccccggtcg gcttcccgtg
     6241 tggagcggcc cggagaacgg ggcgccccgg gaggtgggtg atgcgcacga tcttcttcgt
     6301 ccccgtcggg gccatggccg tggccgccgc gctctcgctc ttcggaccgg gcctcccggc
     6361 cggcccggct cccgcggggg agaccaggct cgagcgcgcc gcgcgggtca tcgccgatga
     6421 gtcgcggacc cccgaggccg tgggggccgc gctccgcgat ctcctcgacg aggcgatcgc
     6481 ggcagcgccg gcctcgggcc tcgccggcga gtggccggcg aaggtcgcac gggcccggga
     6541 gctgctcgcg gtccgcccgc ccgcccatga cgaggcccgc cgtctcctcg cggagagctg
     6601 gcgcgaggcg ttcgggacgg cgtgggcgtt ccccgccgcg gtcgccacga tcgacgacgc
     6661 cacggcgctc gcagagcgga acgtcggcgc ggccgcgacg gcccgccgcg agggacgctc
     6721 cggcgacgcg gtgcgccttc tgctcgagac cgtcatcctc gtcgtcacgc cgatgcacgc
     6781 gcccgccgcg cggtcctgag gcgtccggcg taatctggat gtccgcagtc gagctgggcc
     6841 gggcgctcct cgggcgacgc gagacgggtg ctgcgcccgg acgggacggc cggcgacggg
     6901 gagagcgggt gagcgaagag gacgtcccgg accgggaggc cgaattcgag gagctcgtcc
     6961 accgtcaccg cgaccggatg atccggaccg tgtggcgcgt cctccgcgac gcggacctcg
     7021 cggaggaggc gctccaggat gcgctcggga cgctctggag gaagctcgac acgttgcggg
     7081 cgcacccgaa ccccgaggcc ttcgtcctcc gggtctgcct cgacgcggcg tacgaccggc
     7141 tccgcgagcg ggttcgcgcg cgacgacggg acgccccgga ggacgaggcg gcgcccggac
     7201 ccggcggatc gggcgaccgc gtcgaggtcc gggaggtcct cgaggcgatc cggcgcctag
     7261 accggcggca ggcgacggcg atcctgctgc acgcggtcca gggggagccg taccgggccg
     7321 tcgcggaggc gctcggctgc ggcgaggcga cggcgcgggt ccacgtgcag cgcgcgcggg
     7381 agaagctccg gcgcgccttc gggagaagcg tcgcccgccc gggaaaggag acggagccgt
     7441 gaacgagtgg cgatccgacg acaccttcgg ggcgcgggac gtggacgcgc ggctctcgtc
     7501 cgcacttcgg gacgacgttc cgcccgaggt ggcgcggcgg ctcgagcgcc gggccgcggt
     7561 ggaggtggcg cggcggcgga tccgccgccc gttcctgctc gcgctcctcg accggctcgt
     7621 cccggccccg gccctgacgc ggctcgcgat cccggccggg ctcgtcctcc tcgcgtccgg
     7681 ggcgctgctg cagggatcgg cgtggccctc ggaagcccgg gaggccctcc agcgcctcaa
     7741 cgtgaccgcc tccgcctcgc gggcgcttgg cgaggcgggg ccgctcctct gcggggcggg
     7801 ttcgcccctc gaggggctcg gtcccgaggc cctgtccgac cgagccttcc ggagctggct
     7861 cctcgtcggg acggaggagg tcccggaagg ccggctgcgg ctcgtcttcc gcgcgccgcg
     7921 agagagagcg cgctacgagg tcgtcgtcga cgcggcgtcg ttccggccgc ggacgatccg
     7981 gcggacccgg ctcgacgctc cgcccggggg cgtcccggtg tccgacgagt gcgcctggcc
     8041 cgggaacgac ggacggccgg tcccggaaag gaggtcccga tgaacgtctc cgctccgatc
     8101 gccgggagcg tcttcctctt cctcgtgctc ctcgcgctcg acgggccgga gtggttccgc
     8161 cgggacacga cggacgcggg cgcgaggctc cgcaggctcg ccttcgacgc ctgggccgcg
     8221 ggcgtcctcg cctcgcacct cttcttcgtc ggtgcgctgg gccgcccgga ccggggcctc
     8281 gccgcgacgg cccgggagat gacgctcgcg ttcctcccgg cgctgctcgg cctcggcgtg
     8341 gcggccgcgg ccgggatgcg cgcctttgcg ccttcgggac gcgacgcggc gccggacgcc
     8401 ggcgaggtcc gggccgcggc gccgctcgcg agcgccgcgg ccttcgtggt cctcgtcgcc
     8461 gcggggctgc tcgcggcgac gcacacgggg ggagaggcgc agctcgcgcc ggtcgccgtc
     8521 ctcctccacc cgccggcgct cctcgcgttc gccggcgcgg cgctgctcct cctgcgcctc
     8581 gcgggccggg gcgcgacgcg gaacgccctc gcgcccgcga tcgccctcgc cgctctcctc
     8641 gcgggtctcg cgggcctctt ccaggcgctc tccggttttg ccgcgcgcga cctcgggcgc
     8701 gtgacggccg gactcgcgtt cctcctgacg agcgggttcg cggggctcct cgggctcctc
     8761 ctcgcgtcgc gcgcgccggg agccggcggc cggagagggg gcgcgagcgt cgcgggcggc
     8821 gtcgcccggg ccctcctgcc gctggcggcg ctcctcttcc tcgccttcgc cttcgtcgcc
     8881 gtgttgacgc cgatgaaggc gccgggctga gcgcgtcgac gccgatcggg cccgcgttca
     8941 gcgcgaggcc gccggcggcg gcgcgaggag acgcgcccgg acgatcccga ccggcggcgc
     9001 gagactctgg tccttcgccg gggagcgcca gatcttccgg acgacctcca ttccgcggac
     9061 gacccgcccg aaggcggcga agccctggcc gtcggggttg cgggcgcccc cgaagtcgag
     9121 cgagggctgg gggccgacgc agacgaagaa gtcgctcgtg gcagagtcgg gggtgtcgcg
     9181 ggccatggag acggccccgt cgacgtgcga gaggcccgtg tcgcgggtcc gctcgaggcg
     9241 gatcgggggc cggtcgtcgg cctggcggga gggggcgacg ccggcctgga tcacgtcgat
     9301 cttcaccgtc cggcccggct ggttctccgg cgtcacggtc cggtggaacc gtcccccgtc
     9361 gtaccgcccc gcctcgacgt accagaggaa gttccgcgcg gtcaccgggg cgcggaggat
     9421 aggaggtttg atttttggcg acggcgaggt agagggtcga agctggctcg aacggcgacc
     9481 aggggcggca ccgcggcggt gccgcccccg gtcgcgagcg ttcggggagc gccgagggac
     9541 ttccggggcc cgtctcggag gagggtgagg ggagcctcag cctgcgcgat gggggcggag
     9601 acgaggctcg accgtgaggc cgcggcggct cgccggaggc ggagcgggcc ggtaggccgg
     9661 gccggaggcg ggcggctctg ctttccgggc ctggctgcgg gccttcagct tgccgcggac
     9721 gacgttggag caggcgccgt agtagtgaac gaggtgcctt ctcggatcgg gaacgtgcgc
     9781 gaggagacgg gcgacgaagt cgagggcgtc gagggtctcg gcaattccgc cgtcgtggcc
     9841 gtcgcggggc agataggtgg cggtctgaga ccccggatcc ctgcgcagac gggtgaggct
     9901 cacgggggag cggaggcagt agcgggcgag agcctcgagg gtgcggccgt caccggcggg
     9961 gacggtcact gagttgtgga cggagaagcc ggagtggcgc caggagagga ggaggcgggt
    10021 gcggtcctcg tcgaggaggc cttcgcgctg caggagacgg atcaccttgt gccggaagag
    10081 cgtctcggcg gcggaggggc tgacgtacgg cacgggcacc cactggccgg agacggtcca
    10141 gccgccacgg gtgacgagcg cgtggacgtg gggatggaga ttgagctggc ctccgaacgt
    10201 ctggacgacg gccaccctgc cgggtcggaa ggcctcttcc tcgccggccg ctacggccat
    10261 cagctcgcgg acggcctgcc aggccgcagc acagagaggg ccgaggagct ctcggtgatg
    10321 catgaagtgg gggcgaaggc gcttagggac ggtgaagacc cactgggcgt ggccgacatc
    10381 cgcgaccact tcgtccttca ggaaggcggc gaactcggcg gcgcgcttgg cgccgcagga
    10441 tgggcagagc ccgcgcccct tacacgagaa ggcgagcagg tactcggcgg cgcaatcgtg
    10501 tggctggcct ggttgtctgc ccaccgggcg gccgccttcc tcgcgccgca gggatcccgc
    10561 ggcgagagct gagctcctac tcgaagtacc cgttgacgtc caggatgacg tgggcggtgc
    10621 cggacgcctg ag
//
LOCUS       DAOONL010000114        10528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7159_length_10528_cov_13.138451, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000114 DAOONL010000000
VERSION     DAOONL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_10528_cov_13.138451"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..237)
                     /locus_tag="PLP50_13670"
     CDS             complement(4..237)
                     /locus_tag="PLP50_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52637.1"
                     /translation="MEVISYVVSDIENGTTSLWLCDQDRWLGRVFERDTGWCVELSDL
                     SLLRDPTILDAILDGKEGGVPLNVELRLGEAAG"
     gene            477..2537
                     /locus_tag="PLP50_13675"
     CDS             477..2537
                     /locus_tag="PLP50_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA52638.1"
                     /translation="METGHAFEGKYEVLEKLREGGMGAIYKVRHRLLDQVRVVKVMRP
                     SVAASPDMHSRFLREAQLATRLSHPNIVTFHDFAFDSTETAYMVMEYIEGTSLADLLK
                     TAGRLPLSLVVRVGTEALAALGYLHRRRIVHRDLSPDNVMLAVDEDGSVRTKLIDLGI
                     AKPILEAHERLTATGEFIGKLRYASPEQIELGGSEGLDGRSDLYSLGASLYELATGLS
                     PFRADGIGALLRAHAAGAFLPFEESDPEGRLGGPLRDVLRRSLAPSRDDRYGDADAFS
                     AALQAAHAAQEDPDEAPPWTGYLARGLAAQRRVARAGGGSSAQRAIDSRFPATRPRPT
                     DLTEKERTPVPETRRETAASLPARDATRTQPEAVPLREDDGPDDRTDLVTPTPRPAPA
                     PARDGSARSDRTELHGADVVRALPRARAQGTRRRVLSLAVLLAGFAGAAALVVLLVSP
                     SEDGERPTALPVGPVPEGTGLPALAPALPSPAVGETPHAGGDVTEPSPAPTAAATAPP
                     ATVPAPAVLPTATPVSLPATVPSRSAVVALPTSLPRKLRYCPVVEPTSYAQGSVREEA
                     AGFSGAAYVAPRPDAGRIQIQIEVDPPEPREGEPAEVVARIVNDGDMPLSIRQVEELR
                     ARGGFVPLAAARVPLGVDVGGVLEIYRTRFVLAPGATFLKDLRVVDESGDVWRAGVRI
                     VPCP"
     gene            2935..3111
                     /locus_tag="PLP50_13680"
     CDS             2935..3111
                     /locus_tag="PLP50_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52639.1"
                     /translation="MMYCVPPPALEVRSVGPKLMDEARDSRAFTGAVHSKSSNIDSGK
                     LVPVLKGDVPGTVK"
     gene            complement(3464..3796)
                     /locus_tag="PLP50_13685"
     CDS             complement(3464..3796)
                     /locus_tag="PLP50_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52640.1"
                     /translation="MITELDRKHVAYGLCVLGFTGVGASWGAAGGLVLLGAAGGAVAG
                     LYSCRHLSEPIKRKLFSRTGRLSEDEFRLALIAARREFPFASKPQLLNLIADARIEAA
                     RVPSRYRC"
     gene            complement(3953..4459)
                     /locus_tag="PLP50_13690"
     CDS             complement(3953..4459)
                     /locus_tag="PLP50_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52641.1"
                     /translation="MLKRTLSIPAEAGDLSVDLSLWNGSETVRVGEQVLSRKRSFRLV
                     SDHRFRHPSDPDGPEYRLRVRGVSGHALWRGSRLVSLRERKGLRHFEALGLSCCGLIV
                     IEALLGFASGVSRRDFSGIVEPLPEGLAALLLAVPLSWWMGRRLRAPTRADAGAQEET
                     DARREASS"
     gene            complement(4503..4985)
                     /locus_tag="PLP50_13695"
     CDS             complement(4503..4985)
                     /locus_tag="PLP50_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52642.1"
                     /translation="MTTPTFAIETQRGLPTVTRRFLSGTEVALRGEEELSRQWPWWVF
                     RDHRFRLPDDPDAECHLRLGGRWGYSPWRDDVLLAHRQSALSRFGQAVGVSIPSLLVL
                     DLLLRVESGPGGVRVAPSGPMRWFDFSFPLLVVLLAWPVSWWLGRRLRRRRLAETAAG
                     "
     gene            complement(5340..8651)
                     /locus_tag="PLP50_13700"
     CDS             complement(5340..8651)
                     /locus_tag="PLP50_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPA52643.1"
                     /translation="MAKKTTSPKALLSRSRRILLLGLLGLAPEPSVALAGAEPSARAL
                     EFAWTWWSGSEETGQPGTYGTRGLESATNVPGARLGSATWIDGSGNLWLFGGYGLAET
                     DEGTLNDLWRFSRASKRWTWVGGARTANDPGVYCIGGDFCGTELWPGARRDAFAWTDG
                     SGGLWLYGGAFAGDQRWSDLWRYSTATSTWTLVSGTSAKNEAASYGTRGVPSGTNRPG
                     ARSEGVSWTDDEGNFWLFGGYGNGANGDTRNDLWTRSRVSGYWTWISGENLPGASGSY
                     GTVGVPASTNVPGARAGAAGWKDGDGNLWLFGGMEVHDGFDAYRNDLWRWEITSRRWT
                     WMSGSNLPDQPGVYGSPGIPDANATPGARASATAWRGSAGSGWILGGTPYDCFLACSR
                     NDFWKWDGRQWAWMGGATETDDPGVYGSKGVPNPADRPGSRSGAAAWVDPAGDLWLFG
                     GLANALIGTFGTIELHRNDLWSYDVPAGAPAAANLAVARLSCDGSTSTSISSGEALFA
                     GDALRIETSVAPPHAQSPVLEWRFDYDFHFVHGADDNEAEPRILHPDLTGTQSSPTTP
                     LSLVGPCDPRAGGSPATGSGCWASVTGNGNAGGPDFLTTDPPGTLKYTHLAVWARNAA
                     GSAGTRTFVLNWSLPRVNLQTTTVPLGREALELSAEGCPVSRRWYFGRVAGGGDAPVL
                     DPACTTSSCSHAFPAAGIYSYRLEATYRTGYVSYETGTVTVTQAAEPPAITFTANPLT
                     IAAGQSSTLAWTVTNATTAAIDAGIGAVALPSGTQVVSPTTTTTYTLTATGPGGSSTR
                     AATVTVNPACSAPNAPTAPALRPRFNAAGPVTGTDPLSATWGAPSGGTSPTAYEYRVN
                     GDAYQRVGMQLGADVAPRGANGDQPVQLFVRAVACAPEQTGAVAQSPAYPLSPPVADF
                     DAPAEATVLQPLVFTDRSSQATSWLWIVDEGASPGTQQSFTYAFQTTGTHTVALLASN
                     GNGTNMRTRSINVRPGASSVVATAARSRRLGPSGPDRLGLPGLDLTPPGPALLRLAPA
                     GSDAVVCYLRLLEPDGSLLRERRLVSPSGEDSVYDLKAWTVLERIDLDLACSGPVQAA
                     VEQERSRIPEPLERE"
     gene            complement(8669..9886)
                     /locus_tag="PLP50_13705"
     CDS             complement(8669..9886)
                     /locus_tag="PLP50_13705"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA52644.1"
                     /translation="MGAAVRPETRYAAGYVYFRAANSEKKGFYYVLLEGAPAGLRAAL
                     PRPLPSTTAVEYFLQAIDTARSRERTEQYVPVVKPSGTCAPERAPSPPPNDGLTVGLT
                     EKGQDPVPPGFNSADIARVILASGAVLTLAAALEGAGAGGAAAAGGTGGGAAGAAGGA
                     AAGAAAGGISTGVLIGGGLAVAAGVAVAAGGGGGDDGGSPSTPPSPSVTVGATPTNGQ
                     APLAVQLSATASGGTSPYSWAWNLGDGNLSNLQNPQHTYRGAGTFNVSLTVTDARQRT
                     VSSSVLVTVTAPPPLRFLEADVTWAGGGDLDLAVLRNGAAAGGAGAIRAGCDPSGRSR
                     TERVLVQGAALAPGTYTQLVTAKACAGDTASTGITGVLTVVTDQGSRCQQFFTVGAGG
                     TFTPPQACQVVQP"
BASE COUNT         1471 a         3917 c         3622 g         1518 t
ORIGIN      
        1 ctttcaacca gccgcctctc cgaggcgaag ttcaacattc aacgggacac cgccctcttt
       61 cccgtcgagg atcgcgtcga gaatcgtcgg gtctctgagc aggctgaggt cggaaagctc
      121 gacacaccat cccgtatcgc gttcgaacac cctccccagc caccgatcct ggtcgcagag
      181 ccacagcgac gtcgttccgt tctcgatgtc ggacacgacg tacgaaatca cttccaccgg
      241 catctcctcc ttcggctgtg gtcaggctcg ccgcacgggg atcgccctgt caaccgagag
      301 ggggaggtcc tggcgatcgc gtcgagggga ggagggccag ccgcctccca gaacggcgcg
      361 ttccgggcgc cgggaccggc ccgacggagc gtcacgggac acctgtcgcg cggccggcgg
      421 agaggcgata ctgcgcaccg ccacggccag acggaggtgt agaggaggag acgcgcttgg
      481 agaccggcca cgcgttcgag gggaagtacg aggttctcga gaagctgcgc gagggaggga
      541 tgggcgcgat ctacaaggtc cgccaccgcc tcctcgacca ggtccgcgtc gtcaaggtca
      601 tgcggccgag cgtcgccgcg agccccgaca tgcacagccg gttcctgcgc gaggcccagc
      661 tcgcaacacg gctgagccac ccgaacatcg tcacgttcca cgatttcgcc ttcgattcca
      721 ccgagacggc ctacatggtc atggagtaca tcgaggggac cagcctggcg gacctgctca
      781 agaccgccgg gcgcctcccg ctctccctcg tcgtccgcgt cggcacggaa gcgctggccg
      841 cgctcgggta cctgcaccgg aggcggatcg tccaccgcga cctttccccc gacaacgtca
      901 tgctcgccgt cgacgaggac ggctccgtcc ggacgaagct catcgacctc ggcatcgcca
      961 agccgatcct cgaggctcac gagcgcctca cggcgacggg ggagttcatc ggcaagctcc
     1021 gctacgcctc gcccgagcag atcgagctcg ggggatccga gggcctggac ggccgaagcg
     1081 atctctactc gctcggggcc tcgctctacg agctcgcaac cgggctctcc ccgttccgcg
     1141 ccgacgggat cggcgcgctc ctgcgggccc acgccgcggg cgccttcctg ccgttcgagg
     1201 agagcgaccc ggaggggcgt ctcggcggcc cgttgcgcga cgtcctccgg cgctcgctcg
     1261 caccgtcgcg cgacgaccgc tacggcgacg ccgacgcgtt ttccgccgcg ctgcaggcgg
     1321 cccacgccgc gcaggaggac ccggacgagg cgccgccgtg gaccgggtac ctcgcgcggg
     1381 gcctcgccgc ccagcggaga gtcgcgcgcg ccggaggcgg ctcctcggcg cagcgcgcga
     1441 tcgactcccg cttcccggcg accagaccgc gcccgacgga cctcaccgag aaggagcgga
     1501 caccggtccc cgagacccgg cgcgagaccg ccgcgtcgct tccggcccgc gacgcgacga
     1561 ggacgcagcc cgaggcggtc ccgctccgcg aggacgacgg accggacgac cggacggatc
     1621 tcgtgacgcc gacccctcgg cctgctcccg ctcccgcgcg cgacggaagc gcgcggagcg
     1681 accggacgga gcttcacggg gcggacgtcg tccgcgcgct tccccgcgcc cgcgcccagg
     1741 ggactcgccg ccgcgttctc tctctcgccg tcctcctcgc cgggttcgcc ggcgccgccg
     1801 cactcgtcgt gctcctcgtc tccccgtccg aggacggaga gcgtccgacc gctctccccg
     1861 tgggcccggt tccggaggga accgggctgc ctgcgctcgc gccggccctg ccctccccgg
     1921 cggtcggcga gaccccgcac gccggcgggg acgtcacgga gccctcgccg gcgcctacgg
     1981 ccgccgcgac cgcgccgccc gcgaccgttc cggcacccgc cgttcttccg accgcaacgc
     2041 cggtctcgtt gccggccacg gttccgagcc gctccgcggt cgtcgcgctg ccgacgtcgc
     2101 tgccgcggaa gctccggtac tgccccgtcg tcgagccgac gtcgtacgcg caggggtccg
     2161 tccgcgagga agccgccggg ttctcggggg cggcttacgt cgcacctcgc cccgacgccg
     2221 ggaggatcca gatccagatc gaggtcgacc ctccggagcc gcgagagggc gagccggccg
     2281 aggtcgtggc ccgaatcgtc aacgacgggg acatgcccct ctcgatccgg caggtcgagg
     2341 agcttcgggc gcgcggaggc ttcgtgccgc tggcggcagc ccgggtcccg ctcggcgtcg
     2401 acgtcggcgg cgtcctcgag atctaccgca ctcggttcgt cctcgctccc ggagcgacgt
     2461 tcctcaagga cctccgcgtc gtggacgaga gcggcgacgt ctggagggcc ggcgtccgga
     2521 tcgttccctg cccctgacgc cagagccgcc gggccgaggg ccacgacgcc ggtggcgtcg
     2581 cgacggccct catgaaggcc gggtcgaatg cgacgacttg ccgagcaccg ccccgctcgt
     2641 gtcgcgcgag cagcgcgtcc ccgcgcccga ggctccagcc ccggggttgg ccgatccgaa
     2701 agcggtgctc cggcatgccc gccccgggca ggatcgctag agccgaccga gtcatcgcgg
     2761 gctccgccgg gatcccgtca cgggcaagaa gccccgagcc ggggcgccat cgagaaccgt
     2821 gtgcctcccc aggtcgcgca tccggctcgg accggcctcg ctccgggcgc gctgggtgtg
     2881 caccgctgga gacgctaccg aacccggcgc agtgagctca ttgccgagac cgcgatgatg
     2941 tactgcgtcc cgccgccggc tctcgaggtt cgatccgtcg ggccgaagct gatggatgag
     3001 gctcgggact ccagggcgtt caccggagcc gtgcactcga agagctcgaa catcgactcc
     3061 gggaagctcg tccccgtcct gaaaggggac gtcccgggta ccgtgaagta gacgaacggt
     3121 ttgtctcggg cggcgcccgt gcctcggtga cgtgagaggc gggagccgga cgacaggaat
     3181 cggactcgga ccggggcgtg gaagtcgatc cccctcaggt agtcgagcca gcggaggggg
     3241 tcttcgcgca gtgtgtcgtg gaagaacgcg gcagtgaccc cgagtccctg gactacgacc
     3301 ggagcgcccg gcagcgccag cgcccttcga attcgttcta cgacgtcctc aggcatacgg
     3361 gcctccctcg tctctgcgtc ggtcctgcat tccgccccgg agccggaacg gccgttcacg
     3421 cgcacgcgtc gcggtcactc gcacctcgga aacagggcaa tccctagcat cggtacctgg
     3481 acggtacgcg agccgcctcg atacgagcgt ccgcgatcag gttgagcagc tggggcttcg
     3541 acgcgaacgg gaactcgcgt cgggcggcga tcagggccag ccggaactcg tcctccgaca
     3601 ggcgtccggt tcggctgaac agcttccgct tgatcggctc ggagaggtgc ctgcagctgt
     3661 agagacccgc gaccgctcct cctgccgctc ccagcagaac caggcctccg gccgctcccc
     3721 agctggcgcc gacgccggtg aagccgagca cgcacagccc gtacgccaca tgcttcctgt
     3781 ccagctccgt gatcacccga acatcgtcca tcgcggcctc catactcttc tttccggtgc
     3841 cctgtcaacg cctcggcgtg ccggtcgcgc gagacgacag tggatctcgg ctcctggcca
     3901 cctgcgctgg tcagtttcgt cgcaccacgt cgcgacgcgt ctcctcctgc ggtcacgacg
     3961 aggcctctcg ccgtgcgtcc gtctcttcct gcgcgcccgc gtccgcccgc gtcggagccc
     4021 gaagccggcg acccatccac caggagaggg gcactgcgag gaggagcgcg gcgagcccct
     4081 cgggaagggg ctcgacgatc cccgagaagt cccgccgcga caccccggac gcgaagccga
     4141 ggagggcctc gatgacgatc agcccgcagc acgagaggcc gagggcctcg aagtgtcgca
     4201 ggcccttcct ctcccggagg gagacgagac ggctcccccg ccagagcgcg tgacccgaga
     4261 cccccctgac cctgagccgg tactccggtc cgtccgggtc ggaagggtgg cggaagcgat
     4321 ggtcgctcac gagccggaac gacctcttcc gcgagaggac ctgctccccg actcgtacgg
     4381 tctcggagcc gttccagagg ctgagatcca cggaaaggtc cccggcctcc gccggtatgg
     4441 acagggttct cttcagcacg agttccgcct tcggctccag gcgcgcgatc tcatctccgt
     4501 cgtcaccccg ccgccgtctc cgcgagccgc cgcctccgga gcctccgccc cagccaccag
     4561 gagaccggcc acgcgagcag tacgacgagg agcggaaacg agaagtcgaa ccacctcatc
     4621 ggcccgctcg gggcgacccg aaccccgccc gggccggact cgacccgcag gaggagatcc
     4681 agcaccagca acgacggaat ggacactccg accgcctggc cgaaccggct caaggcactc
     4741 tgccgatggg ccaggaggac gtcgtcgcgc cagggggagt atccccacct gccgccgagc
     4801 cggaggtggc attccgcatc gggatcgtcc gggaggcgga agcggtggtc gcggaagacc
     4861 caccacggcc actggcgaga gagctcttcc tctcctcgca gggcgacctc cgtgccgctc
     4921 aggaagcgcc gtgtcacggt cgggaggcct cgctgcgtct cgatcgcgaa cgtcggcgtc
     4981 gtcatggtct ctccctcgtt cggcctatgc ggcgatccag cgttccgcgg gtccccgggt
     5041 ccgacggctc tgggcgcgcg gcctccgtca tcgcaggacg gcgccgagca cccggccgac
     5101 gaccctgccc gccaccagga acccgagagc cagggcgacg agcccgacga cccgtcgccc
     5161 cgtgtctgct ttcttcggca tcggacttcc tcccgtctcg ccttaccgcc gtctcccctg
     5221 tcgttcccga ggctccgcgg cgagaccggc tcgggacggc cgccgatcgt gacttcgaga
     5281 ccggcgtccc tcggctccca cgcggctccc cgctgcgatc gtccgccgtc ccgtcccgct
     5341 cattcccgct ccagcggctc cgggatccgg gaccgctcct gctccactgc ggcctgaacc
     5401 gggccggagc aggcgaggtc caggtcgatt cgttcgagga cggtccaggc cttcaggtcg
     5461 tacacggagt cctctccaga cggcgacacg agacgacgct cgcggagcag gctgccgtcc
     5521 ggctcgagga gccgcaggta gcagacgacg gcgtcggaac ccgcaggcgc gaggcgcagc
     5581 aacgccggcc ccggcggcgt caggtcgagc ccggggagac cgaggcgatc cggtcccgac
     5641 ggcccgaggc gtcggctccg cgcagcggtg gccacgacgc tggaggcacc gggccggacg
     5701 ttgatcgacc tcgtccgcat gttcgtgccg ttgccgttgc tggcgaggag ggccaccgtg
     5761 tgggtgcccg tcgtctggaa agcgtaggtg aacgactgct gtgtccccgg gctcgcccct
     5821 tcgtcgacga tccagagcca tgaggtcgcc tgcgagctcc ggtccgtgaa gacgaggggc
     5881 tggaggaccg tcgcttcggc cggggcgtcg aagtcggcga ccggcgggga gagcgggtag
     5941 gccgggctct gcgcgacggc gcccgtctgc tccggagcgc acgccacggc cctcacgaag
     6001 agctggaccg gctggtcacc gttcgcgccg cgcggcgcga cgtctgcccc gagctgcatc
     6061 ccgacgcgct ggtaggcatc gccgttcacc cggtactcgt acgcggtcgg ggacgttcct
     6121 cccgagggcg cgccccaggt cgccgagagc gggtcggtcc ccgtcacggg cccggcggcg
     6181 ttgaagcgcg gccgcagagc cggggccgtc ggagcgttcg gcgcgctgca ggccgggttc
     6241 acggtgaccg tggcggcgcg cgtggacgac ccgccgggac cagtcgccgt gagcgtgtac
     6301 gtcgtcgtcg tcgtcgggct gacgacctgc gtccccgacg ggagggccac cgcgccgatc
     6361 cccgcgtcga tcgccgcggt cgtcgcgttc gtcaccgtcc aggcgagggt ggagctctgg
     6421 cccgcggcga tggtcaaggg gttcgccgtg aaggtgatgg ccggcggctc cgccgcctgg
     6481 gtcaccgtga cggtcccggt ctcgtaggag acgtatcccg tcctgtaggt cgcctccagg
     6541 cggtaggagt agatgccggc cgccgggaag gcgtggctgc aggagctcgt cgtgcaggcg
     6601 gggtcgagaa ccggcgcgtc gcctccgccc gcgacccggc cgaagtacca gcggcgcgac
     6661 acggggcagc cttccgcgga gagctcgagc gcctctctcc ccaagggcac ggtcgtcgtc
     6721 tggagattca cgcgcggcag gctccagttc aggacgaagg tcctcgtgcc tgcgctcccg
     6781 gcggcgttcc tcgcccagac ggcgaggtgg gtgtacttga gcgtgcctgg cgggtccgtc
     6841 gtgaggaagt ccggcccgcc cgcgttcccg ttgcccgtca cggacgccca gcaccccgat
     6901 cccgtcgccg ggctgccgcc ggcccggggg tcgcagggcc cgacgaggct caggggcgtc
     6961 gtgggcgagc tctgcgttcc cgtgaggtcc gggtgcagga ttcgcggctc cgcctcgttg
     7021 tcgtccgcgc cgtggacgaa gtggaagtcg tagtcgaatc gccactcgag gacgggcgac
     7081 tgcgcgtgcg gcggcgccac cgacgtctcg atccggaggg cgtcgcccgc gaacagggcc
     7141 tctccggagg agatgctcgt tgaggtcgag ccgtcgcagg acaggcgcgc gacggcgagg
     7201 ttcgccgccg ccggcgcccc ggcgggaacg tcgtaggacc agaggtcgtt gcggtgcagc
     7261 tcgatcgttc cgaaagtacc gatgagcgca ttcgcgagac ctccgaacag ccagaggtcc
     7321 cctgcggggt cgacccacgc ggccgccccg gatcgggatc cgggtctgtc cgcggggttc
     7381 ggcactccct tcgacccgta gacgccgggg tcgtccgttt ccgttgcgcc ccccatccac
     7441 gcccactgcc gtccgtccca tttccagaag tcgttcctgg agcaagcgag gaagcagtcg
     7501 tacggcgtgc ccccgaggat ccagccgctc ccggcggatc cccgccaggc cgttgcgctc
     7561 gcgcgcgcgc ccggcgtcgc gttcgcgtcc ggaattcccg gggaaccgta cacgcccggc
     7621 tggtcgggca ggttcgatcc gctcatccag gtccagcggc ggctcgtgat ctcccaccgc
     7681 cagaggtcgt tccggtatgc atcgaatccg tcgtgaacct ccatcccgcc gaacagccaa
     7741 aggttcccgt cgccgtcctt ccagcccgcg gcgcccgctc gggcgcccgg tacgttcgtc
     7801 gacgccggga caccgacggt cccgtagctt ccggacgcac cggggaggtt ctctccgctg
     7861 atccaggtcc agtatcccga cactcggctt ctcgtccaga ggtcgtttcg ggtgtctccg
     7921 ttcgcaccgt ttccgtagcc gccgaagagc cagaagttgc cctcgtcgtc ggtccaggag
     7981 actccctccg agcgggcgcc gggtcgattc gtgcccgacg gcactccccg ggtcccgtag
     8041 ctcgcggcct cattcttcgc cgacgttcca ctcacgagag tccaggtcga cgtcgcggtg
     8101 ctgtagcgcc agaggtcgct ccaccgctgg tcgcccgcga acgccccgcc gtagagccag
     8161 aggcccccgg agccgtccgt ccacgcgaac gcgtcccggc gagccccggg ccagagctcc
     8221 gtgccgcaga agtcgccgcc gatgcagtaa acgcccgggt cgttcgccgt ccgcgcgccg
     8281 ccgacccacg tccaccgctt cgaggcgcgg ctgaagcgcc acaggtcgtt cagcgtcccc
     8341 tcgtctgttt ccgccaaccc gtagccgccg aagagccaga ggttcccgga accgtcgatc
     8401 cacgtggcgc tcccgagccg ggcgcccggg acgttcgtcg cggactcgag gccgcgggtc
     8461 ccgtacgtcc cgggctgacc cgtctcctcg gagccgctcc accaggtcca ggcgaactcg
     8521 agcgcacgcg cggacggctc cgctcccgcg agggcgacgg acggttccgg ggccagtccg
     8581 agaaggccaa gcaggaggat ccgtcgagaa cgggagagga gggccttcgg agacgtcgtc
     8641 ttcttcgcca ttgccggctc cttcccgctc acggctggac cacctggcag gcctgcgggg
     8701 gcgtgaaggt tccgccggcg ccgaccgtga agaactgctg gcaccggctt ccctggtcgg
     8761 tcacgaccgt caggactccg gtgatgccgg tcgacgccgt gtctccggcg cacgccttcg
     8821 cggtgacgag ctgcgtgtag gtccccggtg cgagcgccgc tccctggacg aggacccgct
     8881 cggtgcggct tcgcccgctc gggtcacagc cggcgcgaat cgcccccgcg ccgcccgccg
     8941 cggcgccgtt ccggaggacc gcgaggtcga ggtccccgcc tccggcccac gtgacgtcgg
     9001 cctcgaggaa gcggagcggc ggcggtgcgg tcaccgtgac gagcaccgag ctgctcaccg
     9061 tccgctggcg cgcgtccgtc accgtgagcg agacgttgaa ggtccccgct ccgcggtagg
     9121 tgtgctgcgg gttctgcagg ttcgagaggt tcccatcgcc gaggttccac gcccacgaat
     9181 agggagacgt ccctcccgat gcggttgcgg agagctggac cgcaaggggc gcctgtccgt
     9241 tcgtgggtgt ggcgccgacc gtgacgctcg gagagggcgg cgtgctcggg ctcccgccgt
     9301 cgtcccctcc gcctcctcca gcggccacgg caactcccgc ggcgacggcg aggccgccgc
     9361 cgatcaggac gccggtcgag atccctccgg ccgcggcgcc cgcggccgct ccgcccgctg
     9421 cacctgcagc gcctcctccg gtcccgccgg ccgcggccgc cccaccggcc ccggccccct
     9481 cgagggcggc ggcgagggtg aggacggcgc cgctcgcgag gatgactctc gcgatgtcgg
     9541 cactgttgaa acccggggga acggggtcct gccccttctc ggtgaggcca acggtcaggc
     9601 cgtcgttcgg cggcggcgag ggtgcgcgct ccggcgcgca cgtcccgctc ggcttgacga
     9661 caggcacgta ctgctccgtc cgctctctcg accgagccgt gtcgatcgcc tggaggaaat
     9721 actcgacggc ggtcgtcgag gggagcgggc gcgggagggc cgccctgagc ccggcgggag
     9781 cgccttccag gaggacgtag tagaagccct tcttctccga gttggcggct cggaagtaga
     9841 cgtagcccgc ggcgtagcgg gtctccggcc ggacggccgc gcccacgtcg ggtcgctcct
     9901 cgggagtcag gcacttcagg gggtcgtggc gtatcgcgag acgcgactcg cccgcggcag
     9961 atgcgggatc cgggacctgt gcgccggccg ggaacgactg gaccgcgacg gccggaacga
    10021 gaagggcgcc gaccacggcg cggaggtacc ccgacgggat tcgctctcgg gccaccttca
    10081 aagcacgacc cgaggcgctt gttctcccga ggaacaatct ccgctcgcag ctcgacgaag
    10141 ggctggccgc ctgcgtatgc cccgacgaca cgaaggaacg ctctcccatc gcttgcctca
    10201 cttcctgcac tcggctctcc gtgaatccgg ctagagcccc gcaacgcacg gcacccctct
    10261 gtttgagaag agcccgagcc accgtccgac gccaccgaga ggccggatcc atcgtccctg
    10321 gtcgttcccg atagccgatg aatatgcgac cgggtttccg cctgtcaagc ggaccacatg
    10381 atctgcgtca tatccgcgat gactttccct cgcctcccga atccgcctct ggttagagtt
    10441 cagcccttca tcgagtctgt ctgacgggga ggactccccg gttccgacct gcatcgccca
    10501 cccgcgctga agcctctgtc ccacgggc
//
LOCUS       DAOONL010000115        10405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7301_length_10405_cov_18.296425, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000115 DAOONL010000000
VERSION     DAOONL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7301_length_10405_cov_18.296425"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="murB"
                     /locus_tag="PLP50_13710"
     CDS             <1..768
                     /gene="murB"
                     /locus_tag="PLP50_13710"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPA52645.1"
                     /translation="AIPLGKGSNVLVPDEGLDAVVFVLSGELAAVSVEAPLVRAGGGA
                     SLMSLAVTARNASLSGTEGLSGIPSSVGGAVRINAGAYGAEIFDLLEEVTLVSRSGAL
                     RVVPAASIPHGYRWSALCDGDDVVASATLRLRPATREEVDARLAEVREKRKKALPTEP
                     NAGSVFKNPPGDHAGRLLEACGLKGERVGGAVVSERHANVIVNAGGATAADVRALMAR
                     MRDAVRDRFGVDLVPEVEDLGRFVTNRNAAASVESGR"
     gene            765..1817
                     /locus_tag="PLP50_13715"
     CDS             765..1817
                     /locus_tag="PLP50_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPA52646.1"
                     /translation="MTVRALILAGGSGTRLWPLSTDDRPKPFLPLSGKASLLRETHER
                     AARLCGESNVLYSARASHAPHLAGEFPGLPAARLVLEPERRNTAPAIALAALAVADED
                     PDGVLVVLPSDQAVQDADELHRALSLAVESARSGDAFLTLGIPPTRPETGFGYLEAGR
                     ETIPGVREVVRFVEKPPLADAERYAVSGNHYWNAGIFAFSVRLLFSEMERLCPDVLAS
                     MRAAHAARRAGDDAAFEAAFRACRSTSIDYAVMEKARAVRTVPSSCGWSDLGSWEAVF
                     EFRGGADGKSVLSGPAIEEGGCGNLVLAGDRPVRLLGLSDVVVVDAPDGLLVMKRGGS
                     DLLRASVEKSLAKGRA"
     gene            1814..2776
                     /locus_tag="PLP50_13720"
     CDS             1814..2776
                     /locus_tag="PLP50_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPA52647.1"
                     /translation="MSAAVPDVVLPAVSRLVSAVRTVYRGPAIAVEHAVVCLLSRGHL
                     LIEDVPGVGKTTLAMALGKALGLGVHRLQFTPDTLPSDILGLTIFNQQSQAFEFHPGP
                     IFTPILVADEINRATPKTQAALLEAMNERGVTVDGETRRLPEPFLVLATQNPIEYLGT
                     YPLPESQLDRFLMRISLGYPAAEEERALLVSGGADRALSTMRPVLTLTELKQAQALVE
                     SVTVAEKLLDYVMALVQRTRSSRDLVLGVSPRGAQGLFRAVQAHALVAGRPFATPDDV
                     KKVAPAVLAHRVLASATGRYEGTGAGRREREAVQKILDEVAVPL"
     gene            2797..3813
                     /locus_tag="PLP50_13725"
     CDS             2797..3813
                     /locus_tag="PLP50_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPA52648.1"
                     /translation="MKLSVLRLRLKVPWDETILVRVTNLGLGYVLTALVVAIGATNTG
                     NNGLYVLLSLLLGVLLVSGVVSRRNVDAVGVALDGPAEVFAGEPARFRVRLENPTKRA
                     KTALLVKVSGKAAPLLFPQVEAGGEAERGVDLVFPRRGRQRVESLLVFSGYPIGLFRK
                     GRLHPVNEERIVFPSPADAAAPAPDHRDASDEGREPRMRGRGAEILKLREAVPGDDPR
                     DIHWPQTARQGRPIIKERASELGGEVVVHLDTFRPASAGPSWDAAFEEAVRRATGLVL
                     TMLARGEKVGLLCGGTFAPPASGPFHRTTLLTALALVAPGAEAPLPLSLPAGTTLYHV
                     RARG"
     gene            3817..5946
                     /locus_tag="PLP50_13730"
     CDS             3817..5946
                     /locus_tag="PLP50_13730"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HPA52649.1"
                     /translation="MAKKPRPDPALLPFRRERILAVGLLSALAPIPLAFTGALPPAVL
                     LVYLAALGVVLAFVKAGRVPCLADRWLNLAGVAYFALFWFGVRFGGRSLLRTALHILL
                     FTAVLKLASIKRERDFSVSLVLATFLLVASMATSTHSTILLFLGGYAAVAWPLLSRWA
                     LWRDLSAAPDEWRRDTRAREIPSRRAVAASLGAAFALAIPMFVLFPRLKTSYIQGLPG
                     GDSGESGFSDTVDTNLYGRLRQNERVFLRVVVEEGPLVEDPRFLRIRLLAHTRFTDGV
                     WRPPADRGRVVALREGNRLSLRPWGGPRPEPTHRLSLEVAPLGIRFLPYPVTALSASA
                     SPELVRRWGVVPLVRDDDGNFRLTVEPPPLMRFEVLSAAEPLVDRTPPEPGDEATRPV
                     GSATIRDWGREVAAGIDPRADPWLFARRIEEHLATRFFYTLDSARWGPSAVEEFLTSR
                     RSGHCETFATAMALVLREHGIPTRLVTGFAGGERGPFGSYYLVRGREAHAWLEAWCGP
                     ERGWVSFDPTPASGRPQVTRLDVVRSVGQFFGNLEFLYGRWILGFAQADQASIATTVR
                     EAIAAAEEKARAFARAASSIAKGDGPGRLLLGVVVAAALAALVLLLARLARGGGGFGT
                     RGLPPASAAYRRLQRLLRRRGGALTPASAPSETLSAADRLGVGGPSREIVRAYVAESF
                     GGRPTSPTEAERLATLLREARAARSPG"
     gene            6009..6746
                     /locus_tag="PLP50_13735"
     CDS             6009..6746
                     /locus_tag="PLP50_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPA52650.1"
                     /translation="MRRSAPVVLALSFLAAGSAAAAEPASKVVEKTLPLAADGRLEID
                     TYKGRVSVAVWDRAEASIRAVVRPDGECDRAAELVERTKVRIEGGGREVHVVSDYDDL
                     PKIWFSFRDDCSSRPFVEYDIRVPRGATLDLDDYKSRISVEGLGGDVSIETYKGVARL
                     TGLSGRLDVETYKGDVRAELEQVAGDLSVETYKGEIELLLPKGSRVALDEEIGRRGRL
                     VAEVEETRSGPRLVAETYKGTIRLREK"
     gene            complement(6762..8162)
                     /locus_tag="PLP50_13740"
     CDS             complement(6762..8162)
                     /locus_tag="PLP50_13740"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HPA52651.1"
                     /translation="MIQNQARRLRATFLASDVAATCAALLAAYVIRFETVWPTPLGVQ
                     PFANYLGLLPVLAILWPVVFYFHRLYPLRRDRSFVDEALAVLIAASLATLLLVGLLSF
                     SRAFSLPRTLLVLFLGLDVLFVSLGRFAIWRYLEKVWSAGVGVRRALVVGAGEAGRAV
                     ADKLLDHPAAGIKPVGFADDDPERRDAEHRGLRVLGTTADVRALLESHQVDTLFLALP
                     VDAYRTMLRILKDVGNEMVDLRFVPDLFQFVTFKAGVEDFDGLPVINLTQRPLEGWNS
                     LVKRSIDVVLSAGGLVGLSVLLPFVALAIWLEDRGPVFYTQERMGLDGRLFRILKFRS
                     MRVGAEDATGAVWAREGDSRRTRVGAFLRSTSLDEVPQLVNILRGDMSLVGPRPERPE
                     FVREFKETYPQYMLRHRVKAGLTGWAQVHGWRGNTSLSKRIEYDLYYIENWSLALDLR
                     ILWLTATRSFRDENAY"
     gene            complement(8181..9308)
                     /locus_tag="PLP50_13745"
     CDS             complement(8181..9308)
                     /locus_tag="PLP50_13745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52652.1"
                     /translation="MKVALVHDWLTGTRGGEKVLRELARLFPGAPLFTLFHFPGSVPP
                     EVEAREVRTTWLQRVVSPRRDYRSLLPVYFLACERWDLSGFDLVLSTSHCVAKAAKRG
                     PRAFHLCYCHTPVRYLHDQFDDYLRGRGTLARGAARLVRAPLRARDVATVPRVDAFLA
                     NSENVRERIARLYGRTSRVVPAPVDDRFYTPAPRERRGLLVVSALAPYKRVDDALAAS
                     ARLGLPLTVAGFGPEERRLRALAGANVTFAGSPSDEELRELYRSAELVLMPGEEDFGI
                     VPLEAQACATPVVALGKGGALETILDGVTGVLYPSPGPEPLAKAIDLARGTRFDGERL
                     VANAARFSEAAFRTRFLDAAREALFAAGRSDLADGLPRAEP"
     gene            complement(9305..>10405)
                     /locus_tag="PLP50_13750"
     CDS             complement(9305..>10405)
                     /locus_tag="PLP50_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPA52653.1"
                     /translation="RLRVALDARKLRDFGIGTYVRGLLGAASARGEHDLVALVRPADA
                     ALLPPGVEAVPCEAREYSLTEPAAVRRALARTRFDVFHAPHYVVPLFPPAATVVTIHD
                     LIHLKRPEHATLPKRLYATTMLRRALRAARVVLTVSEAVRRDLAAFAPARAAKVRAVP
                     NGIGPRFLAPVDAEAIERVRRARGLDAPYVLFLGNDKPHKNLPGLLEAFVRLEEHGLP
                     HRLVLAGGAPERRGARRAAASSAGIEDRLVDLGVVPDEDVVPLLAGASALAMPSFLEG
                     FGLPVLEAQAVGTPVVCSDRGGLPEAGGDAALYADVDDPAALAETLRNLLSDSALSAR
                     LSARGRERARLFTWEATFEKVAAAWRDAAEGA"
BASE COUNT         1350 a         3744 c         3915 g         1396 t
ORIGIN      
        1 gcgatcccgc tcgggaaggg gtcgaacgtc ctcgtccccg acgaggggct cgacgccgtc
       61 gtcttcgtcc tttcgggaga gctggcggcg gtgtcggtcg aggctcctct cgtccgggcc
      121 ggcggcggcg cctcgctgat gtcgctcgcg gtcaccgcgc gaaacgcctc tctctccggg
      181 accgaggggc tctcggggat cccgtcttcg gtcggcgggg cggtgcggat caacgccggg
      241 gcgtacggag cggagatctt cgacctcctc gaggaggtca cgctggtctc gcgctcggga
      301 gcgctccgcg tcgtcccggc agcgtcgatc ccgcacggct accgctggtc ggcgctctgc
      361 gacggggacg acgtcgtcgc ctcggcgacg ctccgcctcc gccccgcgac gcgggaggag
      421 gtcgacgcgc ggctcgccga ggtgcgcgag aagcggaaga aggctctccc gacggagccg
      481 aacgccggct cggtcttcaa gaacccgccc ggcgaccacg cggggcggct cctcgaggcg
      541 tgcgggctga agggggagcg ggtcggcggg gccgtcgtct cggagcgcca cgcgaacgtg
      601 atcgtgaacg ccggcggcgc caccgcggcc gacgtgaggg cgctcatggc ccggatgcgg
      661 gatgcggtcc gcgaccggtt cggggtcgac ctcgttccgg aagtggaaga cctcgggcgt
      721 ttcgtcacca accggaacgc cgccgcctcc gtagaatcgg gacgatgacg gtccgtgcgc
      781 tgatcctcgc cggcgggagc ggcacccgcc tctggcccct ctccaccgac gaccgaccga
      841 agccgttcct cccgctctcg gggaaggcct ccctcctgcg ggagacgcac gagcgcgccg
      901 cccggctctg cggggagtcg aacgtcctct attccgcccg ggcctcgcac gctccgcacc
      961 tcgccgggga gttcccgggg ctgccggcgg cccggctcgt cctcgagccg gagcggcgga
     1021 acaccgcgcc ggcgatcgcc ctcgccgcgc tcgccgtggc ggacgaggac cccgacggcg
     1081 tcctcgtcgt cctcccgtcc gaccaggccg tgcaggacgc cgacgagctc caccgcgccc
     1141 tctccctcgc cgtggagagc gcccgctcgg gcgacgcgtt cctgacgctc ggcatcccgc
     1201 cgacgcgccc cgagaccggc ttcggctacc tcgaggcggg gcgggagacg atcccgggcg
     1261 tccgcgaggt cgtccgcttc gtcgagaagc cgccgctcgc cgacgccgag cgctacgccg
     1321 tctccgggaa ccactactgg aacgccggca tcttcgcctt ctccgtccgc ctcctgttct
     1381 ccgagatgga gcgactctgc cccgacgtcc tcgcctcgat gcgcgcggcc cacgcggccc
     1441 ggcgggccgg ggacgacgcg gcgttcgagg ccgcgttccg cgcctgccgg tcgacctcga
     1501 tcgactacgc ggtcatggag aaggcgcggg ccgtccggac ggtcccatcc tcctgcggct
     1561 ggagcgacct cgggtcgtgg gaggcggtgt tcgagttccg cgggggtgcc gacgggaagt
     1621 ccgtcctctc cggtccggcg atcgaggagg ggggctgcgg gaacctcgtc ctcgcgggcg
     1681 accggccggt ccgcctcctc ggcctctccg acgtcgtcgt cgtcgacgcg ccggacggcc
     1741 ttctcgtgat gaagcgcggg ggctcggacc tcctccgcgc gagcgtggag aagagcctcg
     1801 cgaaggggcg ggcgtgagcg cggcggttcc ggacgtcgtc ctgccggcgg tttcgcgact
     1861 cgtctcggcc gtccggaccg tctaccgcgg gccggccatc gccgtcgagc acgcggtcgt
     1921 ctgccttctg tcgcgcgggc acctcctgat cgaggacgtc ccgggcgtcg ggaagacgac
     1981 gctcgcgatg gcgctcggca aggcgctcgg cctcggggtc caccggctcc agttcacgcc
     2041 cgacacgctc ccatccgaca tcctcggcct gacgatcttc aaccagcagt cgcaggcgtt
     2101 cgagttccac cccgggccga tcttcacgcc gatcctcgtg gccgacgaga tcaaccgggc
     2161 gacgccgaag acacaggcgg cgctcctcga ggcgatgaac gagcggggcg tcacggtgga
     2221 cggcgagacg cggcggctcc ccgagccgtt cctcgtcctg gcgacgcaga acccgatcga
     2281 gtacctcggc acctacccgc tccccgagtc gcagctggac cgattcctga tgcggatctc
     2341 cctcggctac ccggcggccg aggaggagcg cgcgctcctc gtctccggcg gcgccgaccg
     2401 cgccctctcg acgatgcgcc cggtcctgac gctgaccgag ctgaagcagg cgcaggcgct
     2461 ggtcgagtcg gtcacggtcg ccgagaagct cctcgactac gtgatggccc tcgtgcagcg
     2521 gacccgctcg agccgcgacc tcgtcctcgg ggtctcgccg cgcggcgcgc aggggctctt
     2581 ccgggcggtg caggcgcacg cgctcgtggc ggggcggccg ttcgcgacgc ccgacgacgt
     2641 gaagaaggtg gcccccgcgg tcctcgccca ccgggtcctc gcgagcgcga cggggcgcta
     2701 cgaggggacg ggcgcagggc ggcgcgagcg cgaggccgtg cagaagatcc tggacgaggt
     2761 cgccgtcccg ctctgagcgc ggggaggcgg gatgccgtga agctgtcggt cctccggctc
     2821 cgcctgaagg tcccctggga cgagacgatc ctcgtccgcg tcacgaacct cggcctcggc
     2881 tacgtcctga cggccctcgt cgtcgcgatc ggggcgacga acaccggcaa caacggcctc
     2941 tacgtcctcc tctcgctcct cctcggcgtc ctcctcgtct ccggcgtcgt ctcgcggcgg
     3001 aacgtggacg cggtgggggt ggcgctcgac ggcccggccg aggtcttcgc gggcgagccc
     3061 gcccggttcc gggtgcggct cgagaacccg acgaagcggg cgaagacggc gctcctcgtg
     3121 aaggtctcgg ggaaggcggc gccgctcctc ttcccgcagg tcgaggccgg gggcgaggcc
     3181 gagcgggggg tcgacctcgt ctttccccgc cgcgggcggc agcgcgtcga gtcgctcctc
     3241 gtcttttccg gctatccgat cggcctcttc cggaaggggc ggctccaccc ggtgaacgag
     3301 gagcggatcg tctttccgag cccggccgac gcggccgcgc ccgcgccgga ccacagggac
     3361 gcgagcgacg aagggcggga gccgcggatg cgcggccgcg gggcggagat cctgaagctc
     3421 cgcgaggcgg tgccggggga cgacccgcgg gacatccact ggccccagac ggcgcggcag
     3481 gggaggccga tcatcaagga gcgggcctcg gagctgggcg gcgaggtcgt cgtccacctc
     3541 gacacgttcc gccccgcgtc ggccgggccc tcctgggacg cggcgttcga ggaggctgtc
     3601 cggcgcgcga ccggcctcgt cctgacgatg ctcgcgcggg gggagaaggt cggcctcctc
     3661 tgcggcggga cgttcgcgcc gcccgcgagc ggcccgttcc accggacgac cctcctgacg
     3721 gcgctcgcgc tcgtcgcgcc gggggccgaa gccccgctcc cgctctcgct ccccgccgga
     3781 acgacgctct accacgtgcg ggcgaggggc tgaccgatgg cgaagaagcc gcgccccgac
     3841 ccggcgctcc tcccgttccg gcgggagagg atactggccg tcggcctcct ctccgcgctc
     3901 gctccgatcc cgctcgcgtt cacgggggcg ttgccgcccg ccgtgctcct cgtctacctc
     3961 gccgcgctcg gagtcgtcct cgcgttcgtg aaggcggggc gcgtcccctg tctcgcggac
     4021 cgctggctga acctcgccgg cgtcgcctac ttcgccctct tctggttcgg cgtccgcttc
     4081 ggggggcgtt cgctcctccg gacggcgctc cacatcctcc tcttcacggc cgtcctgaag
     4141 ctcgcgtcga tcaagaggga gcgtgacttc tcggtctccc tcgtcctcgc aacgttcctc
     4201 ctggtcgcct cgatggcgac gtcgacgcat tcgacgatcc tcctcttcct cggcggctac
     4261 gcggcggtgg cgtggccgct cctctcgcgg tgggcgctct ggagggacct ctcggccgcc
     4321 ccggacgagt ggcggcgcga cacacgcgcc cgggagatcc cctcccggcg cgcggtcgcg
     4381 gcctcgctcg gcgccgcgtt cgcgctcgcg atcccgatgt tcgtcctctt cccgcggctg
     4441 aagacgtcgt acatccaggg gctgccgggg ggagactccg gggagagcgg cttctccgat
     4501 accgtcgaca cgaacctcta cggacggctc cgccagaacg agagggtctt cctgcgggtc
     4561 gtggtcgagg agggaccgct cgtcgaggat ccgcggttcc ttcggatacg cctcctggcg
     4621 cacacgcgct tcacggatgg ggtctggcgg ccgccggccg atcgcgggcg cgtcgtggcg
     4681 ttgcgcgagg ggaatcgcct ctcccttcgc ccgtggggag gcccccggcc ggagccgacg
     4741 catcgcctct ctctcgaggt ggcgccgctc ggcatccggt tcctgccgta cccggtcacg
     4801 gccctctcgg cgtccgcctc tcccgagctc gtgcgccgct ggggcgtcgt cccgctcgtg
     4861 agggacgatg atgggaactt ccgcctcacc gtggagccgc cgccgctgat gcgcttcgag
     4921 gtcctctcgg cggccgagcc cctggtcgac aggacgccgc ccgagcccgg ggacgaggcg
     4981 acgcgtccgg tcgggtcggc gacgatccgg gactggggcc gggaggtcgc ggccgggatc
     5041 gacccgcgcg ccgacccgtg gctcttcgcg cggcggatcg aggagcacct cgcgacccgc
     5101 ttcttctaca cgctcgactc ggcgcgctgg ggcccctccg ccgtcgagga gttcctgacg
     5161 agccggcgat cggggcactg cgagacgttc gcgacggcga tggcgctcgt cctgcgcgag
     5221 cacggcatcc cgacgcggct cgtgaccggc ttcgccggag gcgagcgggg accgttcggc
     5281 tcctactacc tcgtccgcgg ccgggaggcg cacgcctggc tggaggcgtg gtgcggcccc
     5341 gagcggggct gggtctcctt cgacccgacg cccgcctccg ggagaccgca ggtgacgcgc
     5401 ctcgacgtcg tgaggagcgt gggccagttc ttcgggaacc tcgagttcct ctacggccgc
     5461 tggatcctcg gtttcgccca ggccgaccag gcctcgatcg cgacgaccgt ccgcgaggcg
     5521 atcgcggcag cggaggagaa ggcgcgggcc ttcgcgcggg ccgcctcctc gatcgcgaag
     5581 ggggatggac cgggccgtct cctgctcggt gtcgtcgtcg cggcggcgct cgccgcgctc
     5641 gtcctcctcc tcgcgaggct cgcccgaggc ggcggaggct tcggcacgcg aggactcccg
     5701 cccgcctcgg cggcctaccg ccggctccag cggctcctcc gccggcgggg cggcgccttg
     5761 acgcccgcct cggcgccttc ggagacgctc tcggcggcgg atcgtctcgg cgtgggcgga
     5821 ccctcccgcg agatcgtccg ggcctacgtc gccgagtcgt tcggagggcg gccgacctct
     5881 ccgaccgagg cggaacgcct cgcgacgctc ctgcgcgagg cgcgcgcggc gcgttccccc
     5941 ggctgaggcc ggaaccgggg cggcgggttc tccgtatccg acggagaacg aactcccgag
     6001 gaggccccat gcgccgatcc gctcccgtcg tcctcgccct ctcgttcctc gccgccggct
     6061 ccgccgcagc tgccgaaccc gcctcgaagg tcgtcgagaa gacgctgccg ctggcggcgg
     6121 acggccggct cgagatcgac acgtacaagg gacgggtgtc ggtcgccgtc tgggaccgcg
     6181 cggaggcgtc gatccgcgcg gtcgtcaggc ccgacggcga gtgcgaccgc gcggccgagc
     6241 tcgtcgagcg gacgaaggtt cggatcgaag ggggcggccg cgaggtgcac gtcgtctccg
     6301 actacgacga ccttccgaag atctggttct ccttccggga cgactgctcg tcccggccgt
     6361 tcgtcgagta cgacatccgc gtcccgcggg gcgcgacgct cgacctcgac gactacaagt
     6421 cgcgcatctc ggtcgagggg ctcggcgggg acgtctcgat cgaaacctac aagggggtcg
     6481 cgcgcctcac cgggctctcg ggccgcctcg acgtggagac ctacaaaggc gacgtccggg
     6541 ccgagctcga acaggtcgcc ggggacctga gcgtcgagac ctacaagggc gagatcgagc
     6601 tcctcctccc gaagggctcc cgcgtcgccc tcgacgagga gatcggccgg cgcggccggc
     6661 tcgtcgcgga ggtcgaggag acgcgatccg ggccgcgcct cgtggccgag acctacaagg
     6721 ggacgatccg gctccgggag aagtagcccg gcacccgggc gtcagtaggc gttctcgtcg
     6781 cggaagctgc gggtcgcggt gagccagagg atgcggaggt cgagggcgag gctccagttc
     6841 tcgatgtagt agaggtcgta ctcgatcctc ttcgaaaggc tcgtgttccc gcgccagccg
     6901 tgcacctgcg cccagcccgt gaggcccgcc ttcacgcggt gccggagcat gtactgcggg
     6961 tacgtctcct tgaactcgcg gacgaactcg gggcgctcgg gacgcgggcc gacgagcgac
     7021 atgtcgcccc ggaggatgtt cacgagctgg ggcacctcgt cgagcgaggt gctcctgagg
     7081 aacgctccga cgcgcgtcct tcgcgagtcg ccctcgcgcg cccagaccgc tccggtcgcg
     7141 tcctcggcgc cgacgcgcat cgaccggaac ttcaggatgc ggaagagccg gccgtccagc
     7201 cccatccgct cctgcgtgta gaagaccggg ccccggtcct cgagccagat cgcgagggcg
     7261 acgaacggga ggaggaccga caggccgacg agcccccccg cggagaggac gacgtcgatc
     7321 gaacgcttca cgagcgagtt ccacccctcg agcgggcgct gcgtgaggtt gatgaccggc
     7381 aggccgtcga agtcctcgac ccccgccttg aacgtgacga actggaagag gtcggggacg
     7441 aaccggaggt cgaccatctc gttgccgacg tccttcagga tccggagcat cgtccggtag
     7501 gcgtcgaccg ggagggcgag gaagagcgtg tcgacctggt gcgactcgag tagcgcgcgg
     7561 acgtcggccg tcgttccgag aacgcgcagc ccgcggtgct cggcgtccct ccgctccggg
     7621 tcgtcgtcgg cgaagccgac cggcttgatg ccggccgcgg ggtggtcgag gagcttgtcc
     7681 gccacggcgc gccccgcctc gccggcgccg acgacgagcg cccggcggac cccgacgccg
     7741 gcgctccaga ccttctcgag gtagcgccag atcgcgaaac ggccgagcga gacgaagagg
     7801 acgtccaggc cgaggaagag gacgaggagc gtccgtggca gcgagaaggc gcgggagaag
     7861 gagaggagcc cgacgaggag gagggtcgcg agcgaggcgg cgatgaggac cgcgagagcc
     7921 tcgtcgacga aagaacggtc cctgcggagc gggtagaggc ggtggaagta gaagacgacg
     7981 ggccagagga tcgcgaggac cgggaggagg ccgaggtagt tcgcgaacgg ctggacgccg
     8041 agcggcgtcg gccagacggt ctcgaaccgg atgacgtagg cggccaggag cgccgcgcac
     8101 gtggccgcga cgtcggacgc gaggaaggtc gctcggagcc gccgggcctg gttctggatc
     8161 acgcggtcac cgggcggatt ctacggttcg gcgcggggga gcccgtccgc gaggtcggac
     8221 cgccccgccg cgaagagggc ttcgcgggcg gcgtcgagga accgggtccg gaacgccgcc
     8281 tcggaaaagc gcgcggcgtt cgcgacgaga cgctcgccgt cgaagcgagt gccgcgggcg
     8341 aggtcgatcg ctttcgcgag cggctcgggc ccgggagaag ggtagaggac cccggtgacg
     8401 ccgtcgagga tcgtctcgag agcgcccccc ttcccgagcg cgacgacggg cgtcgcgcag
     8461 gcctgcgcct cgagggggac gatcccgaag tcctcctcgc ccggcatcag gacgagctcg
     8521 gccgagcggt agagctcgcg gagctcctcg tcggacggcg agccggcgaa cgtcacgttc
     8581 gccccggcga gggcgcggag gcgccgctcc tccggtccga agccggcgac ggtgagcggg
     8641 aggccgagcc gagccgaggc cgcaagggcg tcgtcgacgc gcttgtacgg cgcgagcgcc
     8701 gagacgacga ggaggccgcg gcgctcgcga ggcgccgggg tgtagaagcg gtcgtcgacc
     8761 ggcgccggga cgacgcgcga cgtccgcccg tagaggcgcg cgatccgctc ccggacgttc
     8821 tcggagttcg cgaggaacgc gtcgacgcgc ggcaccgtcg ccacgtcacg cgcgcggaga
     8881 ggcgcgcgga cgaggcgcgc cgctccccgc gcgagcgttc cgcggcctcg caggtagtcg
     8941 tcgaactggt cgtggagata gcggacggga gtgtggcagt agcagaggtg gaacgcgcgc
     9001 gggccgcgct tcgcggcctt ggcgacgcag tgggaggtgg agaggacgag gtcgaagccg
     9061 gagaggtccc acctctcgca ggccaggaag tagaccggga ggagcgagcg gtagtcgcgc
     9121 cggggggaga cgacgcgctg gagccaggtc gtcctcacct cccgcgcctc cacctccggc
     9181 gggaccgacc cggggaagtg gaagagcgtg aagagcggcg ccccggggaa gaggcgcgcc
     9241 agctcgcgca ggaccttctc ccctccgcgc gtgccggtca gccagtcgtg gacgagcgcg
     9301 accttcacgc accctccgcc gcgtcccgcc acgccgcggc gactttctcg aacgtcgcct
     9361 cccaggtgaa gaggcgggcg cgctcgcgcc cccgcgccga gagacgcgcg gagagcgcgg
     9421 agtcggacag gaggttccgg agcgtctcgg cgagcgcggc cgggtcgtcc acgtcggcgt
     9481 agagggccgc gtcgcctccc gcctcgggga ggccgccccg gtccgagcag acgaccggcg
     9541 tcccgacggc ctgcgcctcc aggaccggaa ggccgaaccc ctcgaggaag gacggcatcg
     9601 cgagggcgga ggctcccgcg aggaggggga cgacgtcctc gtcggggacg acgccgaggt
     9661 cgacgaggcg gtcctcgatc ccggcggagg aggcggcggc ccgccgcgcc ccgcgacgct
     9721 ccggcgcgcc gccggcgagg acgagccggt gggggagtcc gtgctcctcg agccggacga
     9781 acgcctcgag gaggcccggg aggttcttgt gaggcttgtc gttcccgagg aagaggacgt
     9841 acggcgcgtc cagtccgcgc gcgcgtcgca cgcgctcgat cgcctccgcg tcgaccgggg
     9901 cgaggaagcg cggcccgatc ccgttcggga cggcgcggac cttcgccgcg cgcgcgggtg
     9961 cgaacgccgc gaggtcgcgc cggaccgcct ctgagaccgt gaggacgact cgggccgcgc
    10021 gcagggcccg ccggagcatc gtcgtcgcgt agagccgctt ggggagcgtc gcgtgctcgg
    10081 gccgcttcag gtggatcagg tcgtggatcg tcacgacggt cgcggccggc gggaagagcg
    10141 ggacgacgta gtgcggggcg tggaagacgt cgaagcgcgt ccgcgcgagg gcccgccgga
    10201 cggcggccgg ctccgtgagc gagtactccc tcgcctcgca cggcaccgcc tccacgccgg
    10261 gcgggaggag cgccgcgtcg gcgggcctca cgagcgcgac gaggtcgtgc tccccccggg
    10321 cggaggcggc cccgaggagc ccgcggacgt aggtgccgat gccgaagtcg cggagcttgc
    10381 gcgcgtcgag cgcgacccgc aggcg
//
LOCUS       DAOONL010000116        10392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7313_length_10392_cov_17.614685, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000116 DAOONL010000000
VERSION     DAOONL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7313_length_10392_cov_17.614685"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLP50_13755"
     CDS             complement(<1..650)
                     /locus_tag="PLP50_13755"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPA52654.1"
                     /translation="MSTRPKARRKPVRRVGLVTRTTSGEAIHITRRLEAWLRKRGVEV
                     LHDAESMGARSAMGGLPRAEIGRHVDLLVTLGGDGTLLSVARHPAPGVPILGIDIGTF
                     GYLTACPPDAWEGLLTDALAGNAPVETRRLLAVDVVEPGRPRRRIRVVNDAVIGKSAL
                     SRIATIRVEVEGRQVLRYRGDGVIVSTPTGSTAYNLSAGGPILAPTMPAIILTPICP"
     gene            complement(660..1421)
                     /locus_tag="PLP50_13760"
     CDS             complement(660..1421)
                     /locus_tag="PLP50_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPA52655.1"
                     /translation="MATPRTRLDLLLVARGLAESRSRAEALVLSGRVKVKGVERPKPG
                     TPLLEDAELTVEGPEHPWVSRGGVKLAAGLDAFGIDPSGRVCLDVGASTGGFTDVLLT
                     RGAARVWAIDVGHGQLHAKLRFDPRVVLREGVNARLLTRDDVPEPCSLLVADVSFISL
                     RLILPAVVPLLDPAAEALLLVKPQFESERGEVGKGGIVRDPDVRTRALERVVAAAVAL
                     GLVHVGTVPSPITGADGNVELLAAFRRDPPPSGSD"
     gene            complement(1421..2455)
                     /locus_tag="PLP50_13765"
     CDS             complement(1421..2455)
                     /locus_tag="PLP50_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPA52656.1"
                     /translation="MIPRTMKAALVRAPGQVEIVEMETPRPGPGELLVAMKAVVVCGS
                     DVHPWYVASKAPAVLGHETAGIVAETGPGVVHVRSGDRVFVHHHVPCGACRSCEAGEP
                     VMCPEWKPSRLHPGGLAEYVRVEAPSVARDALLLPPHVSFTGGAFVEPVACALKAVDR
                     ALVRPGDAALVVGLGSNGLLLGWLARLAGAGLLVGSDPDPIRRRLALDHGFDAAIDPS
                     AAGLPEAVRAVSPRGADVVFVVPTSPAAVLPAIEAAAPAARVAFFSPVAPGETWPIAP
                     NAPYFRDLTLRFSYSSGPLETRRALDLIASGALPVERLVTHRLPLARTAEAFALAEAG
                     GATLKVVVEI"
     gene            complement(2452..3468)
                     /locus_tag="PLP50_13770"
     CDS             complement(2452..3468)
                     /locus_tag="PLP50_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPA52657.1"
                     /translation="MRALVCERPGHVALRELPEPVPGPGEVVVRVESALTCGTDLKLI
                     RRGHPKVPFPTVLGHEYCGRVARVGEGAPFAVGERVVSGVSGPCGACHDCRGGRPNHC
                     PTAFDHAVWGTWAEETRIPARVVASTLCRVPDALSSDAAALLDPLASVLRGLSRLPDP
                     SGRTVLVLGSGPIALLFTALLLRRGAGRVVVAGRNAARLAAHASLGAETISARESLGP
                     QVLVRSGARGAEIVVETTGALEVAEEAPSLAARGGMVLLFAGLAREARLAVLAHRVHY
                     DEVTLAGSFHYTPEDTRTALDLLSRGELPVGLLVTSESSLEKHPFVLERLSRGEEMKV
                     AFRP"
     gene            complement(3482..5401)
                     /gene="dxs"
                     /locus_tag="PLP50_13775"
     CDS             complement(3482..5401)
                     /gene="dxs"
                     /locus_tag="PLP50_13775"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPA52658.1"
                     /translation="MTQRPTPLLDRVNWPRDLKPLTLEQLATLSDELRTFLIDSVSKT
                     GGHFASGLGTVELTVALHYVFDTPRDLLVWDVSHQCYPHKVLTGRRDRLHTIRQKGGL
                     SGFTSRAESEYDVFGAAHASTSISAALGMAAARDAKGDDYSVVAVIGDGGLTGGVAME
                     GLNQGGYLKRRLIVILNDNEMSIAPNVGAMSGYLLRIARGQIYKRVREEVGGILKKLP
                     KGERLAEMAEHLEDGLKKALVPGTLFEELGFKYVGPIDGHNLPVLVRTLREAKEYDGP
                     ILLHVRTTKGKGYALAEKDPVFWHGPSPFKVETGEVAKKAAPPSYTQVFADTLADLAR
                     KDERVVAITAAMPDGTGLDRFAKAFPARTFDVGICEQHAVLFAAGMATQGMKPVCAIY
                     STFLQRAYDQVFHDVCLMDLPVVFALDRGGVVGADGPTHHGAYDLSYLRVFPNMHVAA
                     PKDESELRNLVATALDLGRPVAIRYPRGAGHGVALDAEPKLLPVGKGEVLRVGKDGVV
                     LAVGDPVLPALGAAERLAAEGGASLSVLNARWVKPLDTELLAQFVKPGTTVFTVEENA
                     VAGGFGSAVSEALDALGIAARVHRLGIPDRFVPHATQAEQRAELGIDEEGLLRAFRTG
                     TAGAGVVPLRARVAG"
     gene            complement(5424..6323)
                     /locus_tag="PLP50_13780"
     CDS             complement(5424..6323)
                     /locus_tag="PLP50_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPA52659.1"
                     /translation="MTLEAFLTSAKAEAEAALERVLPAPGDPLADAMRYSTLAGGKRL
                     RPALALAFHDVLGPSRAPREEVAESAAALELVHTYSLVHDDLPCMDDDALRRGKPTCH
                     VVYGEATALLAGDALQSLGFEVLASRPRGEAFAPRRAEAVLLLARAIGAGGMAGGQAL
                     DLAASGHAHGELRTAGRLKEIHEKKTGRLLSAACELGAVLAGAPAESRRAAAEYGDAL
                     GVLFQIADDLLDVTASAAVLGKSVGKDEAQEKLTYVSVYGLEGAVAERERALAVTLER
                     ARALEGEPGLLAALAGYAAHRDR"
     gene            complement(6320..6607)
                     /gene="xseB"
                     /locus_tag="PLP50_13785"
     CDS             complement(6320..6607)
                     /gene="xseB"
                     /locus_tag="PLP50_13785"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPA52660.1"
                     /translation="MTSKTKLPKGLTFEKALERLEKIVAELEEPERGLEASLELFEEG
                     VALSRFCRGKLEEIQKRVDVVLKETPDGLVTEPLDADEEPGGGDDGDEEEA"
     gene            complement(6604..8028)
                     /gene="xseA"
                     /locus_tag="PLP50_13790"
     CDS             complement(6604..8028)
                     /gene="xseA"
                     /locus_tag="PLP50_13790"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPA52661.1"
                     /translation="MSFRYRVPRRATSRPAEPTLPFGDGTPEATAIPVSLFVAKLNGV
                     LRASLPDTWVKGEIGEWRVWSSGHAYFTLKDASAALAAVMWASDVARVPFRPEEGMEV
                     LARGRPDVYPRSGKLSFVVSEIQPVGAGALQLALEQLRARLAAEGLFDPARKKKLPLL
                     PRRIGVVTSRQGAAVRDVLKVLSARFPNAHVTVWPVAVQGPSAPVEIARAVEGFSRLG
                     AADVVIVARGGGSRDDLSAFNDEKVVRAVAACRVPTISAVGHEIDVTLTDLAADLRAA
                     TPSQAAELVVARREDFESRIGGLTRELSRTLKGRLLAAHADAARLVHAPALAGFPARA
                     ESARSRLSSAARELLSAVRRLPAAYGERLLRSESAIASWPERAALPLGLARVAAAERA
                     AADRLERSVGGARERLGALAGALSALDPLRVLARGYSVTYREGTKAPLVDAEGVAPGD
                     PLRIVLARGVLRARVTDIPRDGDR"
     gene            complement(8038..9099)
                     /locus_tag="PLP50_13795"
     CDS             complement(8038..9099)
                     /locus_tag="PLP50_13795"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPA52662.1"
                     /translation="MGAHGTRPAADGRRGSPLLGPVALFVLLSVLSALFSTDVLRSLG
                     ELKGLLTFSLVLLVALLVDDVEDAHLLVDALRLTTLYLVLRGLGDWFLLGHDNVDERL
                     SGGLSIYMTYAGLLMVLSLLLAARALTRSRPAASRVADGVLATGAALLVGLTFTRNAY
                     LGLAAGLVVLVLTARPRLGLAVPPLVVLLVLFLPSGVKQRAASTFDRTDAAARDRLAM
                     WRAGAVIVSERPFFGVGPGRIKELYPLYRQPGFVEPSVGHLHDNVIHVAAETGVPSAL
                     AYLALVAAAFSAWPLARDRSRPAVRALARGALAANAALLVAGVFEYNFGDVEVLRLML
                     VVLALPFAAARGTERASDT"
     gene            complement(9246..9503)
                     /locus_tag="PLP50_13800"
     CDS             complement(9246..9503)
                     /locus_tag="PLP50_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPA52663.1"
                     /translation="MAVDPELLAILACPACEPRPPVTLCRLEAGAAAGVVEKYRDKFR
                     DEEPVVTDGLHCAACGRVYPIVSDIPVMLVEEALPAGARAS"
     gene            complement(9529..10314)
                     /locus_tag="PLP50_13805"
     CDS             complement(9529..10314)
                     /locus_tag="PLP50_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPA52664.1"
                     /translation="MKLLFVGDVVGKPGRKALGLLEKVIAREAVDFTVVNVENAAGGF
                     GVTAEIFADLSKLPIDVFTSGNHIWDKKDFVPLLDEIPNLLRPANYPPGNPGRGWAIQ
                     PTPAGIPVGVLNLQGQVFMAATDSPFRVGDACLEEMKAACPELKVVVVDIHAEATSEK
                     QATGWWFDGRVSLVIGTHTHVPTADARVLPQGTAYQGDAGMTGAYEGIIGFDPKNILE
                     RFRFQTPRGMETATRDVRLCGAVVDVDEANGKARSITPIQERL"
BASE COUNT         1458 a         3789 c         3877 g         1268 t
ORIGIN      
        1 gggcagatcg gcgtcaggat gatcgccggc atcgtcggcg cgaggatcgg cccgcccgcc
       61 gagaggttgt aggccgtgga gccggtcggg gtggagacga tgacgccgtc tccccggtag
      121 cggaggacct ggcggccctc cacctcgacg cggatcgtcg cgatccgcga gagcgcggac
      181 ttgccgatca ccgcgtcgtt gacgacccgg atcctccggc gcggccgccc cggctcgacc
      241 acgtcgaccg cgagcagccg gcgcgtctcg accggggcgt tcccggcgag ggcgtcggtc
      301 aggaggcctt cccacgcgtc cggcgggcaa gcggtcaggt acccgaaggt gccgatgtcg
      361 atgccgagga tcggcacgcc cggcgccggg tgacgcgcga cggagaggag cgtcccgtcg
      421 cctccgagcg tgacgaggag gtcgacgtgt ctcccgatct ccgcgcgcgg caaccctccc
      481 atcgcggagc gcgcacccat cgactcggcg tcgtgaagca cctcgacgcc gcgcttgcgc
      541 agccaggctt cgagccggcg ggtgatgtgg atcgcctcgc cgctggtcgt ccgggtgacg
      601 agtcccaccc gccggaccgg cttccggcgc gccttcggtc gcgtcgacat cggccgattc
      661 tagtcgctgc cgctcggcgg cgggtcgcgc cggaacgccg ccaggagctc cacgtttccg
      721 tcggcgcccg tgatcggcga cgggacggtc ccgacgtgga ccaggccgag cgcgacggcc
      781 gcggcgacga cccgctcgag cgcgcgcgtc cgcacgtccg ggtcgcggac gattcctccc
      841 ttcccgacct cgccgcgctc cgactcgaac tgcggcttca cgaggagcag agcctccgcg
      901 gccgggtcga gcagcgggac gaccgccggg aggatcaggc ggagcgaaat gaaggagacg
      961 tcggcgacga ggagcgagca cggctccggg acgtcgtcgc gcgtcaggag ccgcgcattg
     1021 accccctcgc gcaggacgac gcgcgggtcg aagcggagct tcgcgtgcag ctgtccgtgc
     1081 ccgacgtcga tcgcccagac ccgggccgcc ccgcgcgtga gaaggacgtc ggtgaacccg
     1141 cccgtcgagg ctccgacgtc gaggcagacc cgtcccgaag ggtcgatccc gaaggcgtcg
     1201 agcccggccg cgagcttcac gccgccgcgc gagacccagg ggtgctccgg tccctcgacg
     1261 gtcagctccg cgtcctcgag gagaggcgtg ccgggcttgg gccgctcgac gcccttcacc
     1321 ttcacgcgcc ccgacaggac gagcgcctcg gccctcgacc gcgactcggc cagccctcgc
     1381 gcgacgagga ggaggtcgag ccgcgtcctc ggcgtcgcca tcagatctcg acgacgacct
     1441 tcagcgtcgc gccgccggcc tcggcgagcg cgaatgcctc ggccgtccgt gcgagcggga
     1501 ggcggtgcgt gaccagccgc tcgaccggga gcgcgccgga ggcgatgagg tcgagcgcgc
     1561 gtcgggtctc gagcggcccc gaggaatagc tgaagcgaag cgtcaggtcc ctgaagtacg
     1621 gggcgttcgg agcgatcggc cacgtctccc ccggcgcgac gggcgagaag aaggcgaccc
     1681 gcgccgcggg cgccgctgcc tcgatcgcgg gcaggacggc cgcgggcgac gtcgggacga
     1741 cgaagacgac gtcggccccg cgcggcgaga cggcgcggac cgcctcggga agacccgccg
     1801 cggaggggtc gatcgccgcg tcgaagccgt ggtcgagcgc gaggcgccgc cggatcgggt
     1861 cgggatcgct cccgacgagg agccccgcgc ccgcgagccg cgcgagccag ccgaggagaa
     1921 gcccgttcga gccgaggccg acgacgagcg cggcgtcgcc ggggcggacg agggcgcggt
     1981 cgaccgcctt cagcgcgcac gcgaccggct cgacgaacgc gccgcccgtg aacgagacgt
     2041 gcggggggag gaggagagcg tcgcgggcga ccgagggcgc ctcgacgcgg acgtactcgg
     2101 cgaggccgcc ggggtggagg cgcgacggct tccactcggg gcacatgacc ggctctcccg
     2161 cctcgcagga gcggcaggcg ccgcacggga cgtggtgatg gacgaagacc cgatcgccgc
     2221 tccggacgtg gacgaccccc ggccccgtct ccgcgacgat ccccgcggtc tcgtggccga
     2281 ggacggccgg ggccttcgac gcgacgtacc aggggtggac gtcgctcccg cagacgacga
     2341 ccgccttcat cgccacgagg agctcgcccg gcccggggcg cggggtctcc atctcgacga
     2401 tctcgacctg cccgggggcg cggacgagcg cggccttcat cgtgcgcggg atcacggccg
     2461 gaacgccacc ttcatctcct cgccacgcga aagcctctcc aggacgaacg ggtgcttctc
     2521 cagggagctc tccgacgtga ccaggaggcc gacgggaagc tccccccgcg agaggaggtc
     2581 gagagccgtc ctcgtgtctt ccggcgtgta gtggaacgag ccggcgagcg tcacctcgtc
     2641 gtagtggaca cggtgcgcga ggaccgcgag gcgcgcttct cgggccaggc ccgcgaagag
     2701 gaggaccatc ccgccgcgcg ccgcgaggct cggcgcctcc tcggccacct cgagcgcccc
     2761 cgtcgtctcc acgacgatct cggcgccgcg tgcgccgctc cggacgagga cctgcggacc
     2821 caggctctcc cgcgcggaga tcgtctcggc tccgagcgag gcgtgcgccg cgaggcgggc
     2881 ggcgttccgt cccgcgacga cgacgcggcc cgccccgcga cgcaggagga gcgccgtgaa
     2941 cagcagggcg atcggcccgg acccgaggac gaggaccgtc cgccccgacg ggtcgggcag
     3001 tcggctgagg ccacgcagga ccgacgcgag cgggtcgagg agcgccgcgg cgtcggacga
     3061 cagcgcgtcg gggacgcggc agagcgtcga cgcgacgacg cgcgcgggga tccgcgtctc
     3121 ctcggcccag gtcccccaga cggcgtggtc gaaggcggtc gggcagtggt tcggtcgacc
     3181 cccgcggcaa tcgtggcacg ccccgcaggg gccgctgacg ccggagacga cgcgctcgcc
     3241 gaccgcgaac ggcgccccct ctccgacgcg ggcgacccgt ccgcagtact cgtgcccgag
     3301 gaccgtcggg aacggcacct tcgggtgccc ccgccggatg agcttgagat ccgtcccgca
     3361 ggtcagcgcc gactcgaccc ggacgacgac ctcccccggg cccggaacgg gctcgggaag
     3421 ctcccggagg gcgacgtgtc cgggccgctc gcagacgagc gcgcgcatct ctctcgcggc
     3481 gtcagcccgc gacccgcgcc cggagcggga cgacgccggc cccggcggtc cccgtccgga
     3541 acgcccggag gagcccctcc tcgtcgatgc cgagctcagc cctctgctcg gcctgcgtgg
     3601 cgtgcgggac gaagcggtcg gggatcccga gccggtggac gcgggccgcg ataccgagag
     3661 cgtcgagcgc ctcggagacc gccgatccga accctccggc gacggcgttc tcctcgacgg
     3721 tgaagacggt cgtcccgggc ttcacgaact gcgcgaggag ctcggtgtcg agcggcttga
     3781 cccaccgcgc gttgaggacg gagagcgacg cgcccccctc cgccgcgagc cgctcggccg
     3841 cgccgagcgc cggcaggacc gggtccccga ccgccaggac gacgccgtcc ttgccgacgc
     3901 ggaggacctc gcccttgccg accggcagga gcttcggctc ggcgtcgagc gccacgccgt
     3961 gcccggcgcc gcgcgggtag cggatcgcca cggggcggcc gaggtcgagg gccgtggcga
     4021 cgaggttccg gagctcgctc tcgtccttcg gcgcggcgac gtgcatgttc gggaagacgc
     4081 ggaggtagga gaggtcgtag gcgccgtggt gcgtcggccc atccgccccg acgaccccgc
     4141 cccggtcgag cgcgaagacg acggggaggt ccatgaggca gacgtcgtgg aagacctggt
     4201 cgtaggcgcg ctggaggaac gtcgagtaga tggcgcagac cggcttcatc ccctgcgtgg
     4261 ccatgccggc cgcgaagagg acggcgtgct gctcgcagat cccgacgtcg aacgtccgcg
     4321 ccgggaacgc cttcgcgaaa cggtcgaggc cggtgccgtc gggcatcgcc gcggtgatcg
     4381 cgacgacccg ctcgtccttc cgggcgaggt cggcgagcgt gtctgcgaag acctgcgtgt
     4441 agctcggagg cgcggccttc ttcgcgacct cgcccgtctc gaccttgaac ggcgacggcc
     4501 cgtgccagaa gaccgggtcc ttctcggcga gcgcgtatcc ctttcccttc gtcgtccgga
     4561 cgtggaggag gatcggcccg tcgtactcct tcgcctcgcg gagagtccgc acgaggaccg
     4621 ggaggttgtg gccgtcgatc ggcccgacgt acttgaagcc gagctcctcg aagagcgtgc
     4681 cggggacgag cgccttcttc aggccgtcct ccaggtgctc ggccatctcg gcgagccgct
     4741 ctcccttcgg gagcttcttg aggatccccc cgacctcctc ccggacgcgc ttgtagatct
     4801 gcccgcgcgc gatccggagg aggtagcccg acatcgcccc gacgttcggg gcgatcgaca
     4861 tctcgttgtc gttcaggatg acgatgagcc ggcgcttgag gtagcccccc tggttcagcc
     4921 cctccatcgc gacgccgccc gtcaggccgc cgtcgccgat gaccgcgacg acgctgtagt
     4981 cgtccccctt cgcgtcccgc gccgcagcca tgccgagcgc ggccgatatg gaggtcgagg
     5041 cgtgcgccgc gccgaagacg tcgtactccg actcggcccg gctggtgaag cccgagagcc
     5101 cccccttctg ccggatcgtg tggaggcggt cgcgccgccc cgtcaggacc ttgtgcgggt
     5161 agcactggtg gctcacgtcc cagacgagga ggtcccgcgg cgtgtcgaag acgtaatgga
     5221 gcgcgacggt cagctcgacg gtcccgagcc cggacgcgaa gtgcccgccc gtcttcgaca
     5281 ccgagtcgat caggaacgtg cggagctcgt ccgagagggt cgcgagctgc tcgagcgtga
     5341 ggggcttgag gtcccggggc cagttcaccc ggtccaggag cggggtcgga cgctgggtca
     5401 tagaactatc gatgatatcc ggatcaccgg tcccggtggg ccgcgtagcc ggcgagcgcg
     5461 gcgaggagcc ccggctcccc ctcaagcgcc cgtgcccgct cgagcgtcac ggcgagcgca
     5521 cgctcccgct cggcaacggc cccttcgagg ccgtagacgg agacgtaggt cagcttctcc
     5581 tgggcctcgt ccttcccgac gctcttgccg aggaccgcgg cgctcgcggt gacgtcgagg
     5641 aggtcgtcgg cgatctggaa gaggacgccg agcgcgtcgc cgtactccgc ggcggcgcgc
     5701 cggctctccg ccggagcgcc cgcgaggacc gccccgagct cgcacgcggc cgagaggagc
     5761 ctcccggtct tcttctcgtg gatctccttc aggcgccccg cggtccggag ctccccgtgg
     5821 gcgtgtcccg acgcggcgag gtcgagcgcc tggccgccgg ccatcccgcc ggccccgatc
     5881 gcccgggcca ggaggaggac cgcctcggcc cgtcgcggcg cgaacgcctc cccgcgcggg
     5941 cgcgacgcga ggacctcgaa gccgagggac tggagcgcgt cgcccgcgag gagggccgtc
     6001 gcctcgccgt agacgacgtg gcaggtcggt tttccgcgcc ggagcgcgtc gtcgtccatg
     6061 cacgggaggt cgtcgtggac gagcgagtag gtatggacga gctccagcgc cgcggccgac
     6121 tcggcgacct cctcgcgggg cgcccggctc gggccgagga cgtcgtggaa cgccagcgcg
     6181 agcgccgggc ggaggcgctt gcccccggcg agggtcgagt agcgcatcgc gtcggcgagc
     6241 gggtcgcccg gcgcagggag gacacgctcg agggccgcct ccgcttccgc cttcgccgac
     6301 gtgagaaacg cctcgagcgt cacgcctcct cctcgtcgcc gtcgtcgccc ccgcccggct
     6361 cctcgtcggc gtcgagcggc tccgtgacga gcccgtccgg cgtctccttc aggacgacgt
     6421 cgacccgctt ctggatctcc tcgagcttcc cgcgacagaa gcgggagagg gcgacccctt
     6481 cctcgaagag ctccagcgag gcctcgaggc cccgctccgg ctcctccagc tccgctacga
     6541 tcttctccag gcgctcgagc gccttctcga acgtcagccc cttcggcagc ttcgtcttcg
     6601 acgtcatcgg tcaccgtccc tggggatgtc ggtcacccgc gcgcggagga ctccgcgcgc
     6661 gaggacgatc cggagggggt cgcccggggc caccccctcc gcgtcgacga ggggcgcctt
     6721 cgtcccctcg cggtaggtca cggagtagcc ccgggcgagg acccggagcg ggtcgagcgc
     6781 cgacagggcg ccggccaggg cgccgagccg ctctcgagcg ccgccgacgc tccgctcgag
     6841 ccggtccgcc gcggcacgtt cggccgccgc gacgcgcgcg aggccgagcg ggagcgccgc
     6901 gcgctccggc caggacgcga tggccgactc ggagcggagg agccgctcgc cgtaggccgc
     6961 gggcagcctt cgtacggccg agaggagctc ccgcgcggcc gaggagagcc gactccgcgc
     7021 ggattcagcc cgcgccggga agccggccag ggccggagcg tggacgagcc gcgccgcgtc
     7081 ggcgtgcgcg gcgaggagcc gtcccttcag agtccgggag agctcgcgcg tgagtccccc
     7141 gatccgggac tcgaagtctt cccgccgggc gacgacgagc tcggccgcct gggagggggt
     7201 cgcggcccgc aggtcggccg cgaggtccgt cagcgtgacg tcgatctcgt gcccgaccgc
     7261 cgagatcgtc gggacgcggc aggcggccac ggcccggacg accttctcgt cgttgaatgc
     7321 cgagaggtcg tcccgcgacc ctccgccgcg ggccacgatc acgacgtcgg cggcgccgag
     7381 gcgcgagaag ccctccacgg cccgcgcgat ctcgaccggc gccgacggcc cctggaccgc
     7441 gaccggccag accgtgacgt gggcgttcgg gaagcgcgcg gagaggacct tgagcacgtc
     7501 cctcaccgcg gccccctgcc gcgacgtgac gaccccgatc cgtcgcggga ggagcgggag
     7561 cttcttcttc cgcgccgggt cgaagagccc ttcggcggcg agccgcgccc ggagctgctc
     7621 gagggcgagc tggagcgcgc cggcgccgac cggctggatc tcggagacga cgaacgagag
     7681 cttcccgctc cgcgggtaga cgtccggccg cccgcgcgcg aggacctcca tcccttcctc
     7741 ggggcggaac ggcacccgcg cgacgtccga cgcccacatc accgccgcga gcgcggcgct
     7801 cgcgtccttc agcgtgaagt acgcgtggcc ggacgaccag accctccact ccccgatctc
     7861 ccccttcacc caggtgtcgg ggagcgaggc gcggaggacg ccgttgagct tcgcgacgaa
     7921 gagcgagacc gggatcgccg tcgcctccgg ggttccgtcg ccgaacggca gggtcggctc
     7981 cgcgggacgg ctcgtcgctc tccggggcac ccgatagcgg aacgacatcg tccgattcta
     8041 ggtgtccgac gcgcgctccg tcccgcgcgc cgccgcgaag ggaagggcga ggacgacaag
     8101 catcagccga aggacctcca cgtcgccgaa gttgtactcg aagacgccgg ccacgaggag
     8161 ggccgcgttc gccgccagcg cccctcgcgc cagcgcgcgg acggccgggc gggagcggtc
     8221 gcgcgcgagc ggccacgccg agaacgccgc cgcgacgagc gccaggtagg cgagagccga
     8281 cggcacgccg gtctccgccg ccacgtggat gacgttgtcg tgaaggtgcc ccaccgaagg
     8341 ctcgacgaac cccggctgcc ggtagagcgg gtagagctct ttgattcgcc ccggaccgac
     8401 gccgaagaac ggcctctcgg agacgatcac cgccccggcg cgccacatcg cgagccggtc
     8461 ccgggccgcg gcgtcggtcc ggtcgaacgt cgacgccgcg cgctgcttca cgcccgaggg
     8521 gaggaagagg acgaggagca cgacgagcgg gggcaccgcg aggccgagcc ggggccgcgc
     8581 ggtcaggacg agaacgacga ggcccgccgc gaggccgagg taggcgttac gggtgaacgt
     8641 cagcccgacg aggagcgccg ccccggtcgc gagcacgccg tccgcgacgc ggctcgcggc
     8701 cggccggctc cgggtgagcg cccgcgccgc cagcaggagc gagaggacca tcaggaggcc
     8761 cgcgtacgtc atgtagatcg agaggccgcc cgagagccgc tcgtcgacgt tgtcgtgccc
     8821 cagcaggaac cagtcgccga gaccgcgcag gacgaggtag agcgtcgtca gccggagcgc
     8881 gtcgacgagg aggtgcgcgt cctcgacgtc gtcgacgagg agcgccacga ggaggacgag
     8941 cgagaacgtg aggagcccct tcagctcgcc gagcgagcga aggacgtccg tcgagaagag
     9001 cgccgagagg acgctcagga ggacgaagag agcgaccggc ccgaggagcg gcgagccgcg
     9061 tcggccgtcc gcggcggggc gcgtcccgtg cgcgcccatc gcgaggacgg cggcgccgag
     9121 gagggcgagc gccagcgcgc cgttcgcgag cgcgttcgag ggggtcagcg cggcgcaaag
     9181 gacgaggccc gcgaaggccg acctcgcgag cggcgcccgg cgagcgagcg gccccgggag
     9241 gagattcagg acgcgcgcgc cccggcgggc agggcttcct cgacgagcat gaccgggatg
     9301 tccgagacga tcgggtagac ccggccgcag gcggcacagt ggagcccgtc ggtcaccacc
     9361 ggctcctcgt cgcggaactt gtcacggtac ttctcgacga ccccggcggc ggctccggcc
     9421 tcgagccgac agagcgtcac gggtgggcgc ggctcgcagg ccgggcacgc gaggatggcg
     9481 aggagctcgg gatcgacggc catgcgcgaa tgctagcagc aggcggcctc agagccgctc
     9541 ctggatcggg gtgatcgacc gggccttccc gttcgcctcg tccacgtcga cgacggcgcc
     9601 gcagagccgc acgtcgcgcg tggccgtctc cattccgcgg ggggtctgga agcggaaccg
     9661 ctcgaggatg ttcttcgggt cgaacccgat gatcccctcg taggccccgg tcatcccggc
     9721 gtccccctgg taggccgtcc cttgcgggag gacgcgcgcg tcggccgtcg ggacgtgcgt
     9781 gtgggtcccg atgacgaggg agacccgccc gtcgaaccac caccccgtcg cctgcttctc
     9841 cgaggtcgcc tcggcgtgga tgtcgacgac gacgaccttc agctccgggc aggcggcctt
     9901 catctcctcg aggcaggcgt cgccgacgcg gaacggcgag tcggtcgcgg ccatgaagac
     9961 ctgcccctgg aggttcagga cgccgaccgg gatgcctgcc ggcgtcggct ggatcgccca
    10021 tcctcgtccc gggttcccgg gcgggtagtt cgcgggacga aggaggttcg ggatctcgtc
    10081 gaggagcggg acgaagtcct tcttgtccca gatgtggttc cccgacgtga agacgtcgat
    10141 ggggagcttc gagaggtcgg cgaagatctc cgccgtcacg ccgaagccgc ccgcggcgtt
    10201 ctcgacgttc acgacggtga agtcgaccgc ctcccgggcg atgaccttct cgaggaggcc
    10261 gagcgccttg cgccccggct tcccgacgac gtctccgacg aaaaggagct tcacgatccg
    10321 ttcctcccca ggctctgacg aagccgcccc ggggagtccc cggggcggcg ggcttccgtt
    10381 cggctgtccg gg
//
LOCUS       DAOONL010000117        10361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7357_length_10361_cov_21.832331, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000117 DAOONL010000000
VERSION     DAOONL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_10361_cov_21.832331"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLP50_13810"
     CDS             <1..802
                     /locus_tag="PLP50_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52665.1"
                     /translation="PFLAPWIEEKGFPDPKVEARARKDGKGWRVTLTVTQEGRPYHFL
                     GSVAIDAGGRRLVKPFEAKGARTEVAFTVDEKPTRLEFDAGRDLPVPLENPYTFLSFT
                     DEFRHAKVVYGTTRQVEANHTLALRFQTLLADTYSEELPPVVKDSELTETELAASDLF
                     VLGAPSDNALAARLAGKLPATFGPGWFAYGGKTYGRSDDGLYLCLPNPWNPERVVWLF
                     AGNSALQLHQMTKAWGGSLPQWAVYRSDEVRARGFTMPARHVFERLAE"
     gene            complement(759..2855)
                     /locus_tag="PLP50_13815"
     CDS             complement(759..2855)
                     /locus_tag="PLP50_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPA52666.1"
                     /translation="MLPTLLLAASLGLPGGNLLLGRPAEGIGLTGAPAATDGLLAAEG
                     AAAAQAADVGRAVRLADPSAYLVVDLGEVRTVGALLLQADAGDDYRVEGSTDGESWSG
                     LVRIEAGDASGGLRTRRAPVVPSREVRFLLVHAEGGDGTYAVGELAAYVQPPPSWSEP
                     ATSSTSARTGPTEGDRLVVLRALLAGAAALLLAAGLLLRRQGRPEAFARVRDRALLAL
                     GLLSAAAFFGFGRLHGGRLVHVWDSFHYVVGVKYFPELGYTGLYEAALAAERAAGLLP
                     PGTTVPLRNLTTNAIETTSADDAFARWPSRLGPRWEAFVTDVLWFRADSGAETFRRVL
                     LDHGYNATPAWGVLGGFFARLAGPATDGSVRALALLDPLLLLGAWLLVFRAFGFRAAC
                     VALVFWETNFPARFDWNGGSFLRMDWLAAAMSGVALLKMGRPRLAGAALGLSTLLRLF
                     PGALLLGLGAAALFRLARERRLETIRTELRVAQGALLAAAVVLPLSLLAAGGFTSVRW
                     SAWNELAANSRKHLATPLTNNVGLGTTLTWRDATSGRALVFPDAVEPWKGWKETKSAA
                     LREARPLRLALSAAFLAACAWAFRSRPPWAAAAGGAGLVFVLADLTCYYASVLLLLAL
                     LHAEREEAGVVTSLLAAATGAVPFLLTWDDQRYALVGALVLGAFVLLLALLSREEEPG
                     TSPTPRAVRRRAARAS"
     gene            2978..4123
                     /locus_tag="PLP50_13820"
     CDS             2978..4123
                     /locus_tag="PLP50_13820"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPA52667.1"
                     /translation="MLRSTSRLAPALALSLALASPAAAQRLAGPPSGGNQKASVSQWI
                     GPVEVNVTYNSPKVTAPDGTDRRGKIWGGLVPWGMSKDAFGTCGEECPWRAGANENTV
                     FRTSHDVKIEGQPLAAGSYGLFMVPGPEEFTVIFSKNSTSWGHYFYDAKEDALRVKVK
                     PRKAPYTHWMTFDFVERKPSESTVALRWDELEVPVRIEVSDVDGLYLAEIRKQLRNAT
                     GFDWQAWEQAAQFCLQRKTNLPEALKWAEHAATPGFTGQENFRTLSTLSQAQAANGMA
                     DAAKATMQKAMAHPSATAVDLHMYARGLQAQKKTDEAVAVYLLNAKKHPGQWPVEVGL
                     ARAYSAQGKLKDALTAAKKALAQAPDDPNRKGLQGMIEKLTAGKPID"
     gene            complement(4187..6535)
                     /locus_tag="PLP50_13825"
     CDS             complement(4187..6535)
                     /locus_tag="PLP50_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52668.1"
                     /translation="MKPSFSSALVGVCLFLLGGSASAAAQAKTIVPIVLDVTSGASRF
                     TTELSLANPGASAATVALRYTASLGTALGSGTVSETVPAGGQVRYSDAIATLRSKGLA
                     IPTTGPQGGTLVVESPGAAIVATARTTTPVASPAGRAGLAYVGLGATDAAVGSASVFG
                     LRTNAADRSNVAVFSTSEEDVTLRVTAFDGESGASAVVDAARTLPPYGWHQYDEVLRT
                     AGFASGWVVVERLVGGTGSFSAYGVVNDNGTSDGSFVPPARFAASGSALVLPVLVEAG
                     AFLSELVLANGGSEDATFSLTYVESLSPESGAGGTVTIDVPAGRQRIFPDALEELRRR
                     GLAIGPKEPGHAGSVRVEVSGVPLDQAFVAARTASQAPGRGQFGVFTPAVPAGAEAAA
                     EAIVYGLGADALNRTNVAVLHSGDAGSGPVTLRLQAHDGAAGGAAAGSPLDVTLEAGE
                     WAQPDGFFAASGVANGFVRVTRLAGDAPWIAYGVVNDGGAPGERTGDGAFVPAVVPSP
                     PATEGALVVDHTCTDLSRVPARYLEAAKAQLKIGYGHTSHGSQVVTGMEALAAAGSLL
                     DFDSSWGYQPGVFLNDSAFPGANDLGSPDRTAWADATRELLGRAGGCDRNVVVWSWCG
                     QADTGDPGDIETYLSLMSALEAEFPDVRFVYMTGHLVGSGAEGDLHQRNEQIRRYCRE
                     NGKALFDFADIESFDPGGLVDFMRKYATDGCEYDRNGDGNPWSDGNWAEEWIAAHPAH
                     ELTQLAASCGDCAHSERLNCVLKARAFWWLLARLAGWDGVSG"
     gene            6628..7188
                     /locus_tag="PLP50_13830"
     CDS             6628..7188
                     /locus_tag="PLP50_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HPA52669.1"
                     /translation="MPISHVVGFDDAPFPRGARGDVPVVGCVFSGPVFTGVLSGTVRK
                     DGRNATEVLARLVTESKWAPQLQLVMLQGIALAGFNVVDLAALGRLTGLPVLVVARRR
                     PGLVAIREALLSRVPGGARKWALVEAAGPMEACAGVYVQRAGLSLDEAAGVIARTALH
                     GRIPEPLRAAHLVAGGAWGGESRGRA"
     gene            complement(7241..9412)
                     /locus_tag="PLP50_13835"
     CDS             complement(7241..9412)
                     /locus_tag="PLP50_13835"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPA52670.1"
                     /translation="MFARLWIPAATGLGSALLFQVQPLVAKWFLPWFGGGASVWTACM
                     LFFQVVLVGGYLYAHGLTLRLGRRGQAALHLGLLLVAVTGLAAAALSWPSPLTPGPSW
                     RPGLEAPPLPSLLGLLALSTGVPFLTLATTGPLLQAWFVRLAPGRSPYPLYAVSNAGS
                     LVGLLSYPLLVEPRLDVPAQAWLWTGGFAVYVLLVAATAVAQSRAGSLAPETVRPSSD
                     ASAPDSAGRGGSGPAWWIAYAATASLALLAVTNRLTLEVAPVPFLWVVPLAIYLVTFI
                     ACFGRSRRYRKNVWGLLLLGSAASTALALLHGSGLPLPLQIGTLLILLLSLCMVCHGE
                     LASSAPHPSRLTAFYLSISVGGALGGILAAVVAPALFQGLWELPIAAYLGVVLLLASY
                     FREASPRFFGTPARRRATLAASALVFAVLGTGLGWTVFSERSGTILSSRNFYGWFVVD
                     QVEVPNRPGEHYLRLVHGGIVHGAQLDVPHLRRLPTAYFGPNSGVGRAIRSAVKEGED
                     ESRGIRVGVVGFGVGTLGAWARPQDTYRFYEINPDVIALSTGPRPVFTYLSDPGGKKE
                     ITLGDARLSMEREAAQNYDVLVLDAFSSDAIPVHLLTLEAFRLYRSHLAGPRSILAVH
                     VSNDHLDLEPTVRALARATGFYGVTVWERAKGLALTSQWLVLSPSRERLRVPELSRSV
                     EPWDGPERPVRVWTDAYSDVLGTFRLLGTRRTPSREGTTGR"
     gene            9695..>10361
                     /locus_tag="PLP50_13840"
     CDS             9695..>10361
                     /locus_tag="PLP50_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HPA52671.1"
                     /translation="MKKFRVVQWGLGAMGSGMARLMLEKDGLELVGGIDMRPDYVGKD
                     LGEVLGAGRKLGVAVTSDPASVLDKAKVDLVVIATTSWTKEQMPDLKRILSAGINCIS
                     IAEEMSAPEAQSPALAAELDALAKANGVSILGTGVNPGFVLDLLVVALSGVCHKVERI
                     EASRVNDLSPYGPTVMKTQGVGTTPEGFAKGVADGSIVGHVGFPESIRMISEALGLGV
                     TRIE"
BASE COUNT         1616 a         3622 c         3896 g         1227 t
ORIGIN      
        1 gccgttcctc gccccgtgga tcgaggagaa gggcttcccc gacccgaagg tcgaggcgag
       61 ggcgcgaaag gacgggaagg gctggcgcgt gacgctcacg gtgacgcagg aggggcggcc
      121 gtaccacttc ctcgggagcg tcgcgatcga cgcgggcggc cgtcgcctcg tgaagccgtt
      181 cgaggcgaag ggggcgcgca ccgaggtcgc cttcacggtc gacgaaaagc cgacgcgcct
      241 cgagttcgac gcggggcggg acctcccggt cccgctcgag aacccctaca cgttcctcag
      301 cttcacggac gagttccgcc acgcgaaggt cgtctatggg acgacccgcc aggtcgaggc
      361 gaaccacacg ctcgcgctcc gcttccagac gctcctcgcc gacacctaca gcgaggagct
      421 gccgcccgtc gtgaaggact ccgagctgac cgagacggag ctcgccgcga gcgacctctt
      481 cgtcctcggc gcgccgtccg acaacgccct cgcggcgcgc ctcgccggga agctccccgc
      541 gacgttcggg cccggctggt tcgcgtacgg cgggaagacg tacggccgct ccgacgacgg
      601 cctctacctc tgccttccga acccctggaa cccggagcgg gtcgtctggc tcttcgcggg
      661 caactccgcc ctgcagctcc accagatgac gaaggcgtgg ggcgggagcc tcccgcagtg
      721 ggccgtctac cgttccgacg aggtccgcgc gcgagggttc acgatgcccg cgcggcacgt
      781 cttcgaacgg ctcgcggagt aggagatgtg cccggctcct cctcacgcga gaggagcgcg
      841 aggaggagga cgaacgcgcc gaggacgaga gcgcccacga gggcgtagcg ctggtcgtcc
      901 cacgtgagga ggaacgggac cgcgccggtc gcggccgcga gcagcgacgt gacgaccccg
      961 gcctcctcgc gctcggcgtg gaggagcgcg aggaggagga ggacggaggc gtaatagcag
     1021 gtcaggtcgg cgaggacgaa gacgagccct gcgcccccgg ccgccgccgc ccacgggggg
     1081 cgcgagcgaa atgcccaggc gcatgccgcg aggaaggcgg cggagagggc gaggcggaga
     1141 gggcgggcct cgcgcagcgc ggcgctcttc gtttccttcc accccttcca gggctcgacg
     1201 gcgtccggga agacgagcgc ccggccgctc gtcgcgtcgc gccaggtgag ggtggtcccg
     1261 aggccgacgt tgttcgtcag cggggtcgcg aggtgcttcc ggctgttcgc ggcgagctcg
     1321 ttccaggcgc tccagcggac ggacgtgaag ccgcccgcgg cgaggagcga gagggggagg
     1381 acgacggcgg cggcgaggag ggcgccctgt gcgacgcgca gctctgtgcg gatcgtctcg
     1441 agccgccgct cgcgcgcgag ccggaaaagg gccgcggcgc cgagaccgag gaggagcgcc
     1501 cccgggaaga gccggaggag ggtcgagagc ccgagcgccg caccggccag ccgggggcgg
     1561 cccatcttca ggagcgcgac gcccgacatc gcggcggcga gccagtccat ccggaggaac
     1621 gagccgccgt tccagtcgaa gcgcgccgga aagttcgtct cccagaagac gagcgcgacg
     1681 caggcggcgc gaaagccgaa cgcccgaaat acgagcagcc acgccccgag gaggaggagc
     1741 gggtcgagga gggcgagcgc ccggacggag ccgtcggtcg ccgggccggc gaggcgcgcg
     1801 aaaaagccgc cgaggacgcc ccaggcgggc gtcgcgttgt agccgtggtc gaggaggact
     1861 cgccggaacg tctccgctcc ggagtcggcg cggaaccaga ggacgtccgt gacgaacgcc
     1921 tcccagcgcg ggccgaggcg cgaaggccag cgcgcgaacg cgtcgtcggc cgatgtcgtc
     1981 tcgatcgcgt tcgtcgtgag gttccggagc gggaccgtcg ttcccggagg gaggagcccg
     2041 gcggcgcgct cggccgcgag cgccgcctcg tagagtccgg tgtagccgag ctcggggaag
     2101 tacttcacgc cgacgacgta gtggaagctg tcccagacgt ggacgaggcg tcccccgtgc
     2161 agcctcccga atccgaagaa cgcggcggcc gagaggagcc ccagcgccag gagggcgcgg
     2221 tcgcgaacgc gggcgaacgc ttcggggcgt ccctgtcggc gcaggaggag cccggcggcg
     2281 aggaggagcg cggcggcgcc ggcaagtaac gcgcgaagca cgacgagccg gtcgccctcc
     2341 gtcggtcccg tccgagcgga cgtcgacgag gtcgcaggct cggaccacga cggcggcggc
     2401 tgtacgtacg ccgcgagctc gccgaccgcg tacgtaccgt cgccgccctc ggcgtgaacg
     2461 agaaggaagc ggacctcccg ggacgggacg accggcgccc ggcgcgtccg caacccgccc
     2521 gaggcgtccc cggcctcgat ccggacgagc ccgctccagc tctcgccgtc cgtcgagccc
     2581 tcgaccctgt agtcgtcccc ggcgtccgcc tgcaggagga gcgctccgac ggtgcggacc
     2641 tcacccaggt cgacgaccag gtaggccgac gggtcggcca ggcggacggc ccgtcccaca
     2701 tcggccgcct gggcggcggc ggctccctcg gccgccagca ggccgtcggt cgcggccggc
     2761 gctccggtca ggccgattcc ctcggccgga cggccgagca ggagattccc gcccggaagg
     2821 ccgagcgagg cggcgaggag gagcgtcggc aacatggcgt ctcgtgggcc ggaaacccgg
     2881 cggcgccgga agcgtatctc ggacacccgc acatgaagag cggccggtcc agaccccgcc
     2941 ccggggcctg gcgccggcct tgccgaagga ggacccgatg ctccgtagca cgtcccggct
     3001 tgcgccggcc ctcgcccttt ctctcgccct cgcgtcgccg gcggccgccc agcggctcgc
     3061 cggcccgccg agcggcggca accagaaggc ctcggtctcg cagtggatcg gtccggtcga
     3121 ggtgaacgtc acgtacaaca gcccgaaggt gacggctccc gacgggacgg accggcgcgg
     3181 gaagatctgg ggcggcctcg tcccgtgggg gatgtcgaag gacgccttcg gcacctgcgg
     3241 cgaggagtgc ccctggcggg ccggcgcgaa cgagaacacc gtcttccgga cctcgcacga
     3301 cgtgaagatc gaggggcagc cgctcgcggc gggctcgtac ggcctcttca tggtcccggg
     3361 accggaggag ttcacggtca tcttctcgaa gaactccacg agctgggggc actacttcta
     3421 cgacgcgaag gaagacgccc tgcgcgtgaa ggtgaagccg cggaaggcgc cgtacacgca
     3481 ctggatgacg ttcgacttcg tcgagcggaa gccctccgag tcgaccgttg ccctccggtg
     3541 ggacgagctg gaggtcccgg tccggatcga ggtctccgac gtcgacggtc tctacctcgc
     3601 cgagatccgg aagcagctcc gcaacgcgac cggcttcgac tggcaggcgt gggagcaggc
     3661 cgcgcagttc tgcctccagc ggaagacgaa cctccccgag gcgctgaagt gggccgagca
     3721 cgccgccacg ccgggcttca cggggcagga gaatttccgg accctctcga cgctttcgca
     3781 ggcgcaggcg gcgaacggga tggccgacgc cgcgaaggcg acgatgcaga aggcgatggc
     3841 ccacccgagc gcgacggccg tcgacctcca catgtacgcg cgcggcctcc aggcgcagaa
     3901 gaagaccgac gaggccgttg ccgtctacct cctgaacgcg aagaagcacc cgggccagtg
     3961 gccggtcgag gtcggcctgg cgcgcgcgta ctcggctcag ggaaagctga aggacgccct
     4021 caccgccgcg aagaaggccc tcgcgcaggc ccccgacgac ccgaacagga agggcctgca
     4081 gggcatgatc gagaagctca cggcgggcaa gccgatcgac tgagctccgg cggggccgcg
     4141 cggcgacgag gcccgcgcgg tcccgccgtt cccggcgccc cgaccgctac ccgctcaccc
     4201 cgtcccaccc cgcgaggcgt gccagcagcc accagaacgc gcgcgccttc aggacgcagt
     4261 tgagccgctc ggagtgggcg cagtcgccgc aggaggcggc gagctgcgtc agctcgtgcg
     4321 ccgggtgcgc ggcgatccac tcctcggccc agttcccgtc gctccacggg ttgccgtcgc
     4381 cgttccggtc gtactcgcag ccgtccgtcg cgtacttccg catgaagtcg acgagcccgc
     4441 cggggtcgaa gctctcgatg tcggcgaagt cgaagagggc cttgccgttc tcccggcagt
     4501 acctccggat ctgctcgttc cgctggtgga ggtccccctc cgcgccgctc ccgacgaggt
     4561 gccccgtcat gtagacgaac cggacgtcgg ggaactccgc ttcgagcgcg gacatcaggg
     4621 agaggtacgt ctcgatgtct cccgggtcgc ccgtgtcggc ctgcccgcac cacgaccaga
     4681 cgacgacgtt ccggtcgcag cccccggccc ggcccaggag ctcgcgggtc gcgtcggccc
     4741 aagcggtccg gtcggggctc ccgaggtcgt tcgcccccgg gaaggccgag tcgttcagga
     4801 agacgccggg ctggtacccc cacgaggagt cgaagtcgag gagcgacccg gccgccgcca
     4861 gcgcttccat cccggtcacg acctggctgc cgtgagacgt gtggccgtag ccgatcttca
     4921 gctgcgcctt cgcggcctcc aggtaccgcg ccggcacgcg ggagaggtcg gtgcaggtgt
     4981 ggtcgacgac gagagcgccc tccgtcgcgg gcggcgaggg gacgaccgcg gggacgaacg
     5041 cgccgtcgcc ggtccgctcg ccgggcgcgc cgccgtcgtt cacgacgcca taggcgatcc
     5101 agggcgcgtc tcccgcgagg cgcgtcacgc ggacgaagcc gttcgcgacg cccgacgcgg
     5161 cgaagaagcc gtccggctgc gcccactctc cggcctcgag cgtcacgtcg agcgggctcc
     5221 cggcggccgc gccgcccgcg gcgccgtcgt gcgcctggag gcggagcgtg accgggccgg
     5281 agccggcgtc gcccgagtgg aggaccgcga cgttcgtccg gttcagggcg tcggcgccga
     5341 ggccgtagac gatcgcttcc gcggccgctt cggcgcctgc gggaacggcc ggcgtgaaga
     5401 ccccgaactg cccgcgcccg ggcgcctgcg aggccgtccg ggcggcaacg aaggcctggt
     5461 cgagcgggac tcccgagacc tcgacccgca ccgaccccgc gtggccgggt tccttcgggc
     5521 cgatcgcgag gccgcgccgc cggagctcct cgagcgcgtc ggggaagatc cgctgccggc
     5581 ccgccgggac gtcgatcgtc acggtcccgc ccgcgcccga ctccggcgag agggattcga
     5641 cgtaggtgag cgagaacgtg gcgtcctcgc tccccccgtt cgcgaggacg agctcggaga
     5701 ggaacgcccc cgcttcgacg aggaccggca ggacgagggc cgaacccgag gcggcgaacc
     5761 gggccggcgg gacgaaggag ccgtcgctcg tcccgttgtc gttcacgacg ccgtaggccg
     5821 agaaggaacc ggtccccccc acgaggcgtt cgacgacgac ccacccgctc gcgaagccgg
     5881 ccgtccggag gacctcgtcg tactggtgcc agccgtacgg cgggagcgtc cgcgccgcgt
     5941 cgacgacggc gctcgcgccg ctctcgccgt cgaacgcagt gacccggagc gtcacatcct
     6001 cctcgctcgt cgagaagacg gcgacgttcg agcggtcggc ggcgttcgtc cggaggccga
     6061 agaccgacgc gctcccgacg gccgcgtcgg tcgcccccag gccgacgtag gcgagaccgg
     6121 cccgtccggc cggcgaggcg accggcgtcg tcgtcctcgc cgtcgcgacg atcgcggcgc
     6181 cgggcgactc gacgacgagc gtcccgccct gcgggcccgt cgtcgggatc gcgagcccct
     6241 tcgagcggag cgtcgcgatc gcgtcggaat accgcacctg ccctccggcc gggaccgtct
     6301 cggagacggt gcccgagccg agcgcggtgc ccagcgaggc ggtgtagcgg agcgcgacgg
     6361 tcgcggcgga ggccccgggg ttcgcgaggg agagctccgt cgtgaaccgc gacgcgccgg
     6421 aggtgacgtc gagaacgatc gggacgatcg tcttcgcctg ggcggccgcc gaggcgcttc
     6481 cgccgagaag gaagagacaa acgcccacga gcgcggacga aaacgaaggc ttcatcacga
     6541 cctccgggcc ggtccggggg agagtggacc tcggaagctt agccccggag gcgtccgcgg
     6601 ggcggggtga tccgccagaa tcggggcgtg ccaatctccc acgtcgtcgg cttcgacgac
     6661 gccccgttcc cgcggggcgc gcgcggggat gtccccgtcg tcggctgcgt cttctccggg
     6721 ccggtcttca caggagtcct ttcggggacg gtccggaagg acggccggaa cgcgacggaa
     6781 gtcctcgcgc gcctcgtgac ggagtcgaag tgggcgccgc agctccagct cgtgatgctc
     6841 caggggatcg cgctcgccgg attcaacgtc gtcgacctcg ccgccctcgg tcgcctcacg
     6901 ggccttccgg tcctcgtcgt cgcgcggagg cggcccggcc tcgtcgccat ccgcgaggcc
     6961 ctcctgtcgc gagtcccggg tggcgcgcgg aagtgggcgc tcgtcgaggc cgcggggccg
     7021 atggaggcgt gcgcgggcgt ctacgtccag cgcgcgggac tctccctcga cgaggccgcc
     7081 ggggtgatcg cgcggacggc cctccacggc cggatccccg agccgctccg cgccgcgcac
     7141 ctcgtcgcgg gcggggcgtg gggcggggag agccggggac gggcgtgagg gaggggcggg
     7201 gttccgtgcg tcggcctccc ggccggcgtg gccgacgccc ctatcggccc gtcgtcccct
     7261 ccctcgatgg cgtccgacgc gttccgagaa gccggaacgt tccgaggacg tcgctgtagg
     7321 cgtcggtcca gacacggact ggtcgttcgg gaccgtccca gggctccacg gaacgcgaga
     7381 gctcggggac ccggagccgc tcgcgcgacg gggagaggac gagccattgc gaggtgagag
     7441 cgagcccctt ggcgcgctcc cagacggtga cgccgtagaa gccggtcgcg cgagcgaggg
     7501 cccgcaccgt cggctcgagg tcgagatggt cgttcgacac gtggacggcg aggatcgacc
     7561 gcggaccggc gaggtggctg cgatagagcc gaaaggcctc gagcgtcagg aggtggaccg
     7621 ggatcgcgtc gctcgagaag gcgtcgagca cgaggacgtc gtagttctgc gcggcctccc
     7681 gctccatgga gagccgggcg tctcccagcg tgatctcctt cttcccgccc ggatccgaga
     7741 gatacgtgaa gacaggcctg gggccggtcg agagggcgat gacgtccggg ttgatctcgt
     7801 agaagcggta ggtgtcctgc ggtcgcgccc aggccccgag agtcccgacc ccgaagccga
     7861 cgaccccgac ccgaatcccc cgcgactcgt cctccccttc cttcactgcg ctccggatcg
     7921 cgcgcccgac gccgctgttc gggccgaagt acgcggtcgg gagacggcgc aggtggggca
     7981 cgtcgagctg cgcgccgtgg acgatcccgc catgcacgag gcgcaggtag tgctcgccgg
     8041 gtcgattcgg gacctccacc tggtcaacga cgaaccagcc gtagaaattg cgtgaggaga
     8101 ggatcgttcc ggatcgctcg gagaagaccg tccatccgag cccggtcccg agtaccgcga
     8161 agacgagggc ggaggccgcg agcgtcgcgc ggcgccgagc cggtgtaccg aagaaccgcg
     8221 gcgacgcctc acggaagtag gaggcgagga ggaggacgac tccgaggtag gcggcgatcg
     8281 gaagctccca gaggccctgg aagagggccg gcgccacgac cgccgccagg atgccgccga
     8341 gcgctcctcc gacggagatc gagaggtaga acgcggtcag gcgcgacggg tgcggagcgc
     8401 tcgaggcgag ctcgccgtgg cagaccatgc agagggagag gaggaggatg aggagcgtcc
     8461 cgatctgaag cgggagcgga aggcccgacc catgaagcag cgcgagcgcc gtcgaggccg
     8521 cgctgcccag gagaagcaga ccccagacgt tcttccggta gcgccgcgat cggccgaagc
     8581 aggcgatgaa cgtcacgagg tagatcgcga gcggcacgac ccagaggaac ggcaccggcg
     8641 ccacctcgag cgtgagtcgg ttcgtgacgg ccaggagggc gagcgaggcg gtcgccgcgt
     8701 aggcgatcca ccaggcgggg ccggagcccc cgcgccctgc ggaatccggc gcgctcgcgt
     8761 ccgagctcgg tcggaccgtc tccggcgcga gcgagccggc tcgggactgg gcgacggcgg
     8821 tcgccgccac gaggagaacg tacaccgcga agccgcctgt ccagagccac gcctgcgccg
     8881 gcacgtcgag gcgtggttcg acgagcagcg ggtaggagag cagccccacg agcgatcccg
     8941 cgttcgacac ggcgtagagc ggatacggcg aacggccggg ggcgaggcgc acgaaccacg
     9001 cctggaggag ggggccggtc gtggcgagcg tcaggaaggg cacccccgtc gagagggcga
     9061 gcaggccgag gaggctcggg agcggagggg cctcgagccc gggcctccag gatggtccgg
     9121 gggtgagcgg agagggccag gagagggccg cggccgcgag gccggtgact gcgacgagga
     9181 ggagcccgag atgcagcgca gcctgccccc ggcgtccgag ccgcaacgtc aggccgtgcg
     9241 cgtagagata gccccccacg aggacgacct ggaagaagag catgcacgcc gtccagaccg
     9301 atgctccgcc gccgaaccac ggcaggaacc acttcgcgac gagaggctgg acctggaaga
     9361 ggagggcgga accgaggccg gtcgcggccg gaatccacag gcgagcgaac atccgactca
     9421 gtttagcgcc tcgatctcaa cgggaggccg gcgaggcgtc ggtcgtgccg cggcgagacc
     9481 gccacccgac gaatcgctgc gacgggctca cgaaacgacc tcggactcga acgccgcgga
     9541 ccgtccaaga ccgatgccgg tctgcgcgtt ggctggatcg aagcggcctc gacgcgcgga
     9601 ttcccggtag aatccacggt cggacggcgc cactgttcct gcctgcggct ccccgccggg
     9661 cggttcgaac gggcggtctg gcaggaggat tccaatgaag aagttccgtg tggtccagtg
     9721 gggcctcggc gccatgggaa gcggcatggc acgcctgatg ctcgagaagg acggcctcga
     9781 gctcgtcggc ggcatcgaca tgaggcccga ctacgtcggg aaggacctcg gcgaggtcct
     9841 cggcgccggc cggaagctcg gcgtcgccgt gacgagcgat ccggcgagcg tcctcgacaa
     9901 ggcgaaggtc gacctcgtcg tcatcgcgac gacctcctgg acgaaggagc agatgcccga
     9961 cctgaagagg atcctctcgg ccgggatcaa ctgcatctcg atcgccgagg agatgtcggc
    10021 ccccgaggcg cagagccccg cgctcgccgc cgagctcgac gcgctcgcga aggcgaacgg
    10081 cgtctcgatc ctcggcacgg gcgtcaaccc cggtttcgtc ctcgacctcc tcgtcgtcgc
    10141 cctttccggc gtctgccaca aggtggagcg gatcgaggcg agccgcgtga acgacctcag
    10201 cccctacggc ccgaccgtca tgaagacgca gggcgtcggg acgacccccg agggcttcgc
    10261 gaagggcgtg gccgacggct cgatcgtcgg gcacgtcggc ttccccgaga gcatccggat
    10321 gatcagcgag gcgctcggcc tcggcgtcac ccggatcgag g
//
LOCUS       DAOONL010000118        10198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7533_length_10198_cov_17.346446, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000118 DAOONL010000000
VERSION     DAOONL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7533_length_10198_cov_17.346446"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLP50_13845"
     CDS             <1..290
                     /locus_tag="PLP50_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA52672.1"
                     /translation="SALEIEDVLRLHSAVKDAAVVGLPDEEWGERVAAAVVTGGAPLS
                     LDSLRAFARERLAPYKLPTRLLVLDDLPRNAMGKVTKAAVRTLFGAPPPGR"
     gene            305..1906
                     /locus_tag="PLP50_13850"
     CDS             305..1906
                     /locus_tag="PLP50_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPA52673.1"
                     /translation="MPYLVRSSGAGAPDERFPLKPGGNTVGRSRENDVVLLDASLSRV
                     HARIDVEPDGSSVLDLGSRNGTFVGGLRVLQCRLKHGDTLQLGELPLRFEEETSPARS
                     GYSSPEMTLHGLIGAGAEIDKTSAIQLRGALPMQRSEAKLKILLTVSQILSSPEPVDA
                     VLPRIVELLLQILDVHRVALLLMEEGSDEPVAKVVRSRKTMTESDSFFSRSIVRHVFE
                     RGEGLVSDDALVDPRFAGSATVAGDSIRSSMAAPLKAGERVIGVLYVDHRSIPNRYSR
                     EDLEFLGAFGSQAAQAIENARLTAKLQEEAVRRSSLLRFFPPSVVGPLMGSADFGARV
                     RDADVTVLFSDISGFTAMSSELAPREVVDLLNRYFPVMADIVFRHEGTLEKYIGDALM
                     AIWGVPAPHDDDADRALSAALEMRRALERLNAAWGEGRRLEIHVGLNSGPVAFGNIGS
                     PQYVQFAAIGDTTNVSARVCTAAQPGEVLISDATRRRLTTSRFALEPLPPVAVKGKTE
                     PLLLHRARWAAPEETREIDRSAPRS"
     gene            complement(1812..4916)
                     /locus_tag="PLP50_13855"
     CDS             complement(1812..4916)
                     /locus_tag="PLP50_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA52674.1"
                     /translation="MDREPENGGAHASGAHEAETLVRPVGVADPDAPAGATPPTAGVP
                     PAETTRACPSFPVSGWDRYELVGFLGEGGMGRVFSARDPRLGRDVALKFLKDEDPELA
                     RRFLVEARAQARVEHPSVCPVWEVGEVAGHLYIAMPLLKGRNLKDAAKGMSLEQIVEV
                     VARVADGLHAAHRIGLVHRDVKPGNVLVETTPDGESRPWVMDFGLVRDASVEGMTVTG
                     LALGTPWYMPPEQALGRRAAIDRRSDVYSLGVTLYELLAGTLPFAGETPFDVMNRVLT
                     EEPAPPSRHAPGLPADLETIVLKCLEKEPARRYDSARALADDLRRFLDGEPIAARPTG
                     PVARLLRRARRNPALAILAAGLTLALVSFATFAVRARVQSARRAEIARALGEEVRETE
                     ILVRWISHLLPLHDVNRERAIVRYRMRDLEARLPSMGAAAAAPGAYALGRGHLVLGEL
                     DAARERLQRAWDGGVRTPEAAYALGVNLAHLYERERRSFSAAPTPQERERRRKEADAA
                     LRDPALDFLEKARGARMDSPEYAEALVAELQGDLDRAIDLARSSAESKPWLYEARLLL
                     GRALRRRGLERMEAGRDAEALPDWDAAEEAYRGAVEIARSAPDGYQGLCSLYRDRAET
                     AQIRGRSATKELEGAARWCEGAVEVDPSNALALLRASDVEWQWGIELANEGKDPRARF
                     EKAVALAERTIPSRFDLSYALDNVGIAWVNRAQWEAANGIDPAPSFAKAEEAFRRCSV
                     EFPNLFSVHCNLAETLNARARFGLSRGRDPSPLLAEARRALERGEDFAEHFCHALGWV
                     VSASVESQWALLSGRDPGPAAGEAARRLELLRRVNPGAVTGLLEAATASLAVAEDRLL
                     RGDAPTEELEAARRLLVEASATSAEGRGTALVRAETHLLAARSGRTASWSDGQAAFAE
                     ARRLAPANAHVLSRSARFLRFRLDTPATRPGDAREGLHLARAALEIDPASAEAFLLAG
                     FFSGRLGAADDAARLVARGSVLNPLLPRLLGLSSAARSDRSPASPPAPPSGPGGGGAA
                     RSSP"
     gene            complement(4930..6039)
                     /locus_tag="PLP50_13860"
     CDS             complement(4930..6039)
                     /locus_tag="PLP50_13860"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPA52675.1"
                     /translation="MMARVRNIAINTGGGDAPGLNAVICGVVSAALNRGWRCFGIRDG
                     YNGLLFPEQFPDGGLVPLSRTRVEGIASLGGTILGTTNRGNPLRFPVKQPDGTTVEVD
                     RTDELVRAFALHEIDALVSIGGDGSLAIANVLAKKGVRVVGVPKTIDNDLDRTVITFG
                     FDTARSFATEAIDRLHSTAEAHRRIMVVEVMGRYAGWIALDSGVAGNADAILIPEIPY
                     DLERVAEHLRRAEESGQRHAIVVVAEGAKPKGGTVSTLGPREAGAMERLAGAGERVMK
                     GLEELTGREVRTVVLGHLLRGGTPSSFDRLLALRFGAAAVRALEEGQSGVMVALAPPT
                     VNYVPLEEATSRMKSVPLDCDTIKTARDLAISFGD"
     gene            complement(6081..7418)
                     /locus_tag="PLP50_13865"
     CDS             complement(6081..7418)
                     /locus_tag="PLP50_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPA52676.1"
                     /translation="MLTAECATPRPLLGSARWEWWEWWLERKTRWVRDEVKQLDEGVF
                     RTLAAGTGLPAIHQRSTFPFKDVRDGADRFLGMAKGGERPYARIYTRLGNPTTEYLER
                     VLFQLEAHHVIEKALAVDEKEPTIGALIFGSGMGAISTLALAVCRAGDAILAGNVYGC
                     TDSLFRGLGKFGVEAVFCDMGNVAAVEAALEAHPNVAMVFLESPENPTLRIADIEAIS
                     RLTEPRGVLLVVDNTFCSPYLQQPFRLGADLVMHSLTKFVNGHSTSIGGALLGPFRFM
                     KTDFFPWYKDVGATPSPFDSWLNGMTVQSLAPREAQQSATALEVARFLSTHPKVASVG
                     YPGLPDFPQADLVRRQMRSGGAILTFEVKGGVAAGETVMNYFGRKDTPMELAVSLGSC
                     ITYIQHPASMTHAVVPEADRLARGITPGLIRLSVGLEGAEVLTEHLGRALDLT"
     gene            complement(7428..8498)
                     /gene="rsmH"
                     /locus_tag="PLP50_13870"
     CDS             complement(7428..8498)
                     /gene="rsmH"
                     /locus_tag="PLP50_13870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPA52677.1"
                     /translation="MDEPRPRRRPRYPGRNPRRFEEKYKERDPARFPETVAKVVASGK
                     TPAGTHRPVLVEEVVAALRPAAGEIAVDATLGYGGHAAALLPRLLPGGRLVGLDADPV
                     ELPRTEARLRDLGFAEEALTVRRSSFAGLPKVLSSLGLDAVDLLLADLGVSSMQLDDP
                     ARGFTFREDGPLDMRMNPVRGLSAAQLLARVSEARLAALLEENADEPHAAAIARAIVT
                     TRAASPVDTTAALADAVRRALAALPERVRQEEGDDPVRRTFQALRIEVNDELGALRAL
                     LTALPACLAPGGRAAILTFHSGEDRLVKKAFQAGARAGVFSDWSREPVRPGPEEVRAN
                     PRARPAKLRWALRAETDARPAF"
     gene            complement(8564..>10198)
                     /locus_tag="PLP50_13875"
     CDS             complement(8564..>10198)
                     /locus_tag="PLP50_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPA52678.1"
                     /translation="LDYRPYFVAGLKQLLDEGHVERNAHYAHLNTETGPGHASLGTGA
                     PPRVTGIVANRWFQPRADGSLRPVYCTDQPYVDPETRVEGFRPGPGNLRVPTLGDRLL
                     EKYPAARVVSLSGKDRGAIFMAGKRREHAVYWWDQRTGRFVSSPAYDVRSPGGAVVAG
                     IVDRYNRTRAGGHLPRRIGLAWRKMADPACLPPPSPGTPRPVPELEMRPFQIPVNGLG
                     WDKDMSLASNGYFHGLYYSPFIDELTIDLALEVLASKELELGHEEQPDILCLSLSGQD
                     TVSHAYGAESEENLDALRRLDVQLGRLLETLGRTFPDGKVVLALSADHGFQTIPEREA
                     RVDKTFTGGRILTGNGAVTNFEERLNRYVCEELCLPSGSRPIFGNEGFDVKYNLPALP
                     TMRTVAGPCGEAGRPVTRDDVDRVLPGAIARLWHEEFRTVLLASKKESWDRSDRDVRF
                     ALNDHDAERSGEAILIPRRGVILYPDARGGTHGSHYVYDTHVPLLFWGGGVAAGVSDA
                     ERTPYDFAPTVGKLLGVALPDAVGTAIELPRQTAKP"
BASE COUNT         1367 a         3593 c         3847 g         1391 t
ORIGIN      
        1 tctcagcgct cgagatcgag gacgtcctgc ggcttcactc cgccgtgaag gacgccgcgg
       61 tcgtgggcct ccccgacgag gagtggggcg agcgcgtcgc ggctgcggtc gtgacgggcg
      121 gcgcgccgct ctccctcgac tccctccggg cgttcgcccg cgagcggctc gctccctaca
      181 agctcccgac gcgcctgctc gtcctcgatg acctcccgcg gaacgcgatg gggaaggtga
      241 cgaaggcggc cgttcggacg ctcttcggcg ctccgccacc cggccgctag aatcccgtgg
      301 aacgatgccc tatctcgtac ggtcctcggg cgccggagct cccgacgagc gctttccgct
      361 gaagccgggc ggcaacaccg tcgggcgctc ccgcgagaac gacgtcgtcc tcctcgacgc
      421 gagcctgtcg cgggtccacg cccggatcga cgtcgagccg gacgggtcga gcgtcctcga
      481 cctcggaagc cgcaacggga cgttcgtcgg cgggctccgc gtcctccagt gccggctcaa
      541 gcacggagac acgctgcagc tcggggagct cccgctccgg ttcgaggagg agacgtcccc
      601 ggcccggtcg ggctactcct ccccggagat gacgctccac ggcctgatcg gggcgggcgc
      661 ggagatcgac aagacgtcgg cgatccagct ccgcggcgcg ctgccgatgc agcgctccga
      721 ggcgaagctg aagatcctgc tgaccgtctc gcagatcctc tcctcgcccg agccggtcga
      781 cgcggtcctg ccgcggatcg tcgagctcct cctccagatc ctcgacgtcc accgcgtcgc
      841 cctcctcctc atggaggagg ggtcggacga gccggtcgcg aaggtcgtgc ggtcgcggaa
      901 gacgatgacc gagagcgact cgttcttcag ccggagcatc gtgcggcacg tcttcgagcg
      961 gggggagggg ctcgtcagcg acgacgcgct cgtcgacccg cgcttcgccg gctcggcgac
     1021 ggtcgccgga gactccatcc gctcctcgat ggcggcgccc ctgaaggcgg gcgagcgggt
     1081 gatcggcgtc ctctacgtcg accaccgctc gatcccgaac cgctactcga gggaggacct
     1141 cgagttcctc ggcgcgttcg gcagccaggc cgcgcaggcg atcgagaacg cgcggctgac
     1201 cgcgaagctc caggaggaag ccgtccggcg ctcgagcctc ctgcgcttct tcccgccgtc
     1261 cgtcgtcggt cccctcatgg gctcggccga cttcggcgcc cgggtgcgcg acgccgacgt
     1321 gacggtcctc ttctcggaca tcagcggctt cacggcgatg tcctccgagc tggcgccccg
     1381 cgaggtcgtc gacctcctga accgctactt tcccgtgatg gccgacatcg tcttccgcca
     1441 cgaggggacg ctcgagaagt acatcggcga cgcgctgatg gcgatctggg gcgtccccgc
     1501 cccgcacgac gacgacgcgg accgggccct ctcggccgcg ctcgagatgc gccgcgcgct
     1561 cgagcggctg aacgccgcct ggggcgaggg gcggcggctc gagatccacg tcggcctcaa
     1621 ctccggcccc gtcgcgttcg ggaacatcgg ctctccgcag tacgtccagt tcgccgcgat
     1681 cggcgacacg acgaacgtct cggcgcgcgt ctgcacggcc gcgcagccgg gcgaggtcct
     1741 gatctcggat gcgacccggc ggcgcctgac gacgtcgagg ttcgcgctcg agccgctgcc
     1801 gccggtcgcg gtcaagggga agaccgagcc gctcctcctc caccgggccc gctgggcggc
     1861 gccggaggag acgcgggaga tcgatcggag cgcgccgcgg agctgaggcc gaggaggcgt
     1921 ggaaggagcg ggttcaagac ggagccccgc gccaccagcc gcgccgcgtc gtcggcggcg
     1981 ccgagccgac cggagaagaa gcctgcgagg agaaacgcct cggcggacgc ggggtcgatc
     2041 tcgagcgccg cgcgggcgag gtggagcccc tcgcgcgcgt cgccgggcct cgtcgcgggc
     2101 gtatccagcc ggaagcggag gaagcgcgcg gagcgcgaga ggacgtgcgc gttcgcgggc
     2161 gcgaggcggc gcgcctccgc gaacgcggcc tgtccgtcgg accacgaagc ggtccggccc
     2221 gaccgcgccg cgaggaggtg agtctcggcg cgtacgagcg ccgttccccg tccctccgcg
     2281 ctcgtcgcgg aggcctcgac gaggagacgt cgcgcggcct cgagctcctc cgtcggggca
     2341 tccccgcgca ggaggcggtc ctcggcgacc gcgagcgacg ccgtcgcggc ctccaggagg
     2401 cccgtcaccg caccggggtt caccctccgg agtagctcca ggcgccgcgc cgcctcgccc
     2461 gcggccggac cgggatctcg cccggacagc agcgcccact gcgactcgac cgacgcggag
     2521 acgacccagc cgagagcgtg gcagaagtgc tcggcgaagt cctccccgcg ctcgagcgcg
     2581 cgccgggcct ccgcgagcag gggcgacggg tcgcgcccgc gggagagacc gaaccgcgcc
     2641 cgcgcgttga gcgtctccgc gaggttgcag tggacggaga agaggttcgg gaactcgacc
     2701 gagcaacgcc ggaacgcctc ctccgccttc gcgaaggagg gtgcggggtc gatgccgttc
     2761 gcggcctccc attgggcacg gttgacccag gcgatgccga cgttgtcgag ggcgtaggag
     2821 aggtcgaacc gcgagggaat cgtccgctcg gcgagggcga ccgccttctc gaagcgcgcg
     2881 cgtgggtcct tcccttcgtt cgcgagctcg atgccccact gccattcgac gtccgaggca
     2941 cggagcaggg cgagggcatt cgagggatcg acctcgaccg cgccctcgca ccaccgggcg
     3001 gccccctcga gctccttcgt tgcggagcga ccccggatct gcgcggtttc agcccggtcc
     3061 cggtagagcg agcagaggcc ctgatagccg tccggagcgc tccgtgcgat ctccacggcc
     3121 ccgcgatagg cctcctcggc ggcgtcccag tcggggagcg cctctgcgtc ccggcccgct
     3181 tccatccgtt ccagcccgcg gcgccggagc gcccggccga ggaggaggcg cgcctcgtag
     3241 agccacggtt tcgattcggc cgaagagcgg gcgaggtcga tcgcacggtc gaggtcgccc
     3301 tgaagctcgg ccacgagcgc ctcggcgtac tcgggagagt ccatccgcgc gcctcgcgcc
     3361 ttctcgagga agtcgagcgc cgggtcgcgg agagcggcgt cggcctcttt ccgccgccgt
     3421 tcccgttcct ggggcgtcgg cgctgcggag aaggagcggc gctcccgctc gtagaggtgc
     3481 gcgaggttga cgccaagggc gtaggcggcc tcgggtgtcc tcaccccgcc gtcccaggca
     3541 cgttggagac gctcccgcgc cgcgtcgagc tcgccgagga cgaggtgccc ccggccgagg
     3601 gcgtaggcgc ccggggcggc ggcggcggcg cccatcgaag ggaggcgggc ctcgaggtcg
     3661 cgcatccggt agcggacgat cgcccgctcg cggttcacgt cgtgaagcgg caggaggtgg
     3721 gagatccagc ggacgaggat ctcggtctcc cggacctcct ccccgagagc ccgggcgatc
     3781 tcggcgcgcc gcgcggactg gacgcgcgcg cggacggcga acgtcgcgaa ggagacgagg
     3841 gcgagggtca gtcccgccgc gaggatggcg agcgcggggt tccgccgcgc ccggcggagg
     3901 aggcgggcga cggggcccgt cggacgggcg gcgatcggct cgccgtcgag gaagcgccgg
     3961 aggtcgtcgg cgagggcccg cgcggagtcg taccgccgcg ccggctcctt ctcgaggcat
     4021 ttcaggacga tcgtctcgag gtcggcggga agccccggcg cgtgacggga cgggggagcg
     4081 ggctcctcgg tcaggacccg gttcatgacg tcgaagggcg tctcgccggc gaacgggagc
     4141 gttcccgcga ggagctcgta gagcgtgacc ccgagcgagt agacgtcgga gcggcggtcg
     4201 atcgccgccc tccgtccgag cgcctgctcg ggcggcatgt accacggtgt cccgagggcg
     4261 aggccggtca cggtcatccc ttcgacgctc gcgtcgcgga cgaggccgaa gtccatgacc
     4321 cacgggcggc tctcgccgtc gggcgtcgtc tcgacgagga cgttccccgg cttgacgtcg
     4381 cgatggacga gaccgatccg gtgcgcggcg tggaggccgt ccgcgacgcg cgcgacgacc
     4441 tcgacgatct gctcgagaga catccccttt gcggcgtcct tcaggttccg gcccttcagg
     4501 agcggcatcg cgatgtagag gtggcccgcg acctcgccca cctcccagac cggacagacg
     4561 gaggggtgct cgacgcgcgc ctgggcccgt gcctcgacga ggaagcgccg cgcgagctcg
     4621 gggtcctcgt ccttgaggaa cttgagggcg acgtcccggc cgaggcgcgg gtcgcgggcg
     4681 gagaagacgc ggcccattcc cccctcgccg aggaagccga cgagctcgta acggtcccag
     4741 cccgagacgg ggaacgacgg gcatgcgcgg gtcgtctccg ccggcgggac tccggccgtc
     4801 ggcggggtgg ctccggccgg ggcgtcggga tccgcgacgc ccaccggccg gaccagggtc
     4861 tcggcctcgt gagcgccgct cgcgtgggcg ccgccgttct cgggctcgcg gtccatcgcc
     4921 gccggcggcc tagtctccga agctgatggc gaggtcccgc gcggtcttga tcgtgtcgca
     4981 gtcgagcggg acggacttca tgcggctcgt cgcctcctcg agcgggacgt agttcaccgt
     5041 cggcggggcg agcgcgacca tgacgccgct ctgcccctct tcgagcgccc ggacggcggc
     5101 tgcgccgaag cggagcgcga ggaggcggtc gaaggaagac ggcgtcccgc cgcggaggag
     5161 gtggccgagg acgacggtcc gcacctcgcg ccccgtcagc tcctcgagcc ccttcatcac
     5221 gcgctccccg gcgcccgcga gccgctccat cgcgcccgcc tcgcgcgggc cgagcgtcga
     5281 gaccgtcccc cccttcggct tcgccccctc ggcgacgacg acgatggcgt ggcgctggcc
     5341 cgactcctcg gcgcgccgca ggtgttccgc caccctctcg aggtcgtacg ggatctcggg
     5401 gatcaggatg gcgtcggcgt tcccggcgac gcccgagtcg agggcgatcc acccggcgta
     5461 gcgccccatc acctcgacga ccatgatccg gcggtgcgcc tcggccgtgg agtggagccg
     5521 gtcgatcgcc tccgtcgcga acgagcgcgc cgtgtcgaag ccgaacgtga tgacggtccg
     5581 gtccaggtcg ttgtcgatcg tcttcgggac gccgacgacc cggaccccct tcttcgcgag
     5641 gacgttcgcg atcgcgagcg agccgtcgcc gccgatcgag acgagggcgt cgatctcgtg
     5701 gagggcgaag gcgcggacga gctcgtctgt ccggtcgacc tcgactgtcg tcccgtcggg
     5761 ctgcttcacg gggaaccgca gggggttccc gcggttcgtc gtcccgagga tcgtcccgcc
     5821 gagggaggcg atcccctcga cacgcgtccg gctgagcggg acgagccccc cgtcggggaa
     5881 ctgctcgggg aagagcaggc cgttgtagcc gtcccggatg ccgaagcagc gccagccgcg
     5941 gttcagggcg gcgctcacga cgccgcagat cacggcgttc aggccggggg cgtcgccgcc
     6001 cccggtgttg atcgcgatgt tccggactcg ggccatcatg cgtccccctt cccgccccgc
     6061 agggcggacg aagcgcgagc tcaggtcagg tcgagcgccc tcccgaggtg ctcggtgagg
     6121 acctccgccc cctcgagccc gacggagagc cggatcaggc cgggcgtgat cccgcgggcg
     6181 agccggtccg cctccgggac gacggcgtgc gtcatcgagg ccgggtgctg gatgtaggtg
     6241 atgcaggagc cgagggagac ggcgagctcc atcggcgtgt ccttgcggcc gaagtagttc
     6301 atcaccgtct cgccggcggc gacgcccccc ttcacctcga acgtcaggat cgccccgccg
     6361 gagcgcatct ggcggcggac gaggtccgcc tgggggaagt ccggcaggcc ggggtagccg
     6421 accgacgcga ccttcgggtg cgtcgagagg aagcgggcga cctcgagcgc ggtggcgctc
     6481 tgctgggcct cccgcggggc gaggctctgg accgtcatgc cgttcagcca cgagtcgaac
     6541 ggggaaggcg tggcgccgac gtccttgtac caggggaaga agtcggtctt catgaaccgg
     6601 aacggcccca ggagggcgcc gccgatcgag gtcgagtggc cgttcacgaa cttcgtcagc
     6661 gagtgcatga cgaggtcggc gccgagccgg aacggctgct ggaggtaggg cgagcagaac
     6721 gtgttgtcga cgacgaggag gactccgcgc ggctcggtga ggcgcgagat cgcctcgatg
     6781 tcggcgatcc ggagcgtcgg gttctccggc gactccagga agaccatcgc gacgttcggg
     6841 tgcgcctcga gcgcggcctc gacggccgcg acgttcccca tgtcgcagaa gaccgcctcg
     6901 acgccgaact tcccgagccc ccggaagagg ctgtcggtgc agccgtagac gttccccgcg
     6961 aggatcgcgt cgccggcccg gcagaccgcg agggcgagcg tcgagatcgc ccccatcccc
     7021 gagccgaaga tcagggcgcc gatcgtcggc tccttctcgt ccaccgcgag ggccttctcg
     7081 atgacgtggt gggcctcgag ctggaagagg acccgctcga ggtactcggt cgtcgggttg
     7141 ccgaggcggg tgtagatccg ggcgtagggg cgctcgcctc ccttcgccat cccgaggaag
     7201 cggtcggcgc cgtcgcggac gtccttgaac gggaacgtgg agcgctggtg gatcgcgggg
     7261 aggccggtgc ccgcggcgag ggtccggaag accccctcgt cgagctgctt cacctcgtcc
     7321 cgcacccagc gggtcttccg ttcgagccac cactcccacc attcccagcg ggccgatccg
     7381 aggaggggtc tgggggtcgc gcactctgca gtcagcatgg ggcgatctta gaacgcggga
     7441 cgagcgtccg tctccgcgcg gagagcccat cgcagcttcg cgggccgcgc gcgcgggttc
     7501 gcccggacct cctccgggcc cggccgaacc ggctcgcggg accagtcgga gaagacgccg
     7561 gccctcgccc cggcctggaa cgccttcttc acgagccggt cctcgccgga gtggaacgtc
     7621 aggatggcgg cgcgcccgcc gggcgcgagg cacgcgggga gcgccgtcag cagggcgcgg
     7681 agggcgccga gctcgtcgtt cacctcgatc cgtagcgcct ggaacgtccg ccgcacgggg
     7741 tcgtccccct cctcctgccg gacgcgctcc ggtagcgcgg cgagggcccg acggacggcg
     7801 tccgcgaggg ccgcggtcgt gtcgaccggt gaggccgcgc gtgtggtgac gatcgcccgg
     7861 gcgatcgccg cggcgtgcgg ctcgtcggcg ttttcctcga ggagcgcggc gaggcgcgcc
     7921 tcggagacgc gcgcgaggag ctgcgcggcg gagaggccgc ggacggggtt catccgcatg
     7981 tcgagcgggc cgtcctcccg gaacgtgaag ccgcgcgcgg ggtcgtcgag ctgcatcgag
     8041 gagacgccga ggtcggcgag gaggaggtcg accgcgtcga gcccgaggct ggaaagcacc
     8101 ttcgggagcc ccgcgaagct cgaacggcgg acggtgaggg cctcctcggc gaacccgagg
     8161 tcgcgcaggc gcgcctcggt tctggggagc tcgacggggt ccgcgtcgag gccgacgagc
     8221 cggccgcccg ggaggaggcg cggcagaagc gccgcggcat ggccgccgta tccgagcgtc
     8281 gcgtcgacgg cgatctcgcc ggctgccggg cgcagcgccg cgacgacctc ctcgaccagg
     8341 acggggcggt gcgtcccggc cggcgtcttg cccgacgcga cgaccttcgc gaccgtctcc
     8401 gggaagcggg ccgggtcacg ctctttgtac ttctcctcga accggcgtgg gttgcgtccc
     8461 gggtaacgag gtcgccggcg gggtcggggc tcgtccacgg tccgattgtg cgacggccgc
     8521 ggtccggtcc gccgcgacgg ttccgtcgca gcagaccgca ctctcagggc ttcgcggtct
     8581 ggcggggaag ctcgatcgcc gtcccgacgg cgtcgggaag cgccacgccg agcagcttcc
     8641 cgacggtcgg agcgaagtcg tacggcgttc gctcggcgtc ggagaccccg gccgcgacgc
     8701 cgccgcccca gaagaggagc gggacgtgcg tgtcgtagac gtagtgggag ccgtgggtcc
     8761 cgccgcgcgc gtcggggtag aggatcacgc cgcgccgagg gatcaggatc gcctcgcccg
     8821 accgctccgc gtcgtggtcg ttcagggcga aacggacgtc gcgatcgctt cggtcccagc
     8881 tctccttctt cgaggcgagg aggaccgtcc ggaactcctc gtgccagagc cgggcgatcg
     8941 ccccggggag gacacggtcg acgtcgtccc gcgtcacggg ccgcccggcc tcgccgcacg
     9001 ggcccgcgac ggtccgcatc gtcggcaggg ccgggaggtt gtacttcacg tcgaagccct
     9061 cgttcccgaa gatcggacgc gagccggacg ggaggcagag ctcctcgcag acgtagcggt
     9121 tgaggcgctc ctcgaagttc gtcacggcgc cgtttcccgt caggatccgg ccgcccgtga
     9181 acgtcttgtc gacccgggcc tcgcgctcgg ggatcgtctg gaagccgtgg tcggcggaga
     9241 gcgcgaggac gaccttgccg tcggggaagg tacgcccgag cgtctcgagc aggcgtccga
     9301 gctggacgtc cagccgccgg agcgcgtcga ggttctcctc ggactcggcg ccgtacgcgt
     9361 gggagacagt gtcctgcccc gagagcgaga ggcagaggat gtcgggctgc tcctcgtgcc
     9421 cgagctcgag ctccttcgag gcgaggacct cgagggcgag gtcgatcgtc agctcgtcga
     9481 tgaacgggct gtagtagagg ccgtggaagt agccgttcga ggcgagggac atgtccttgt
     9541 cccagccgag gccgtttacg gggatctgga acggacgcat ctcgagctcg gggaccggac
     9601 ggggcgtgcc cggcgacggc ggcgggaggc aggcggggtc ggccatcttc cgccaggcga
     9661 ggccgatccg tcgcggcagg tgcccgcccg cccgcgtccg gttgtagcga tcgacgatcc
     9721 cggcgacgac ggcgcccccc ggcgagcgga cgtcgtaggc gggggagctg acgaagcgtc
     9781 cggtcctctg gtcccaccag tagacggcat gctcccggcg cttgccggcc atgaagatcg
     9841 cgccgcggtc cttgcccgag agcgagacga cgcgtgcggc ggggtacttc tcgagcagcc
     9901 ggtcgccgag ggtcgggacc ctcaggttgc ccggcccggg gcggaagccc tcgactctcg
     9961 tctccgggtc gacgtacggc tgatcggtgc agtagacggg gcggagggag ccgtcggcgc
    10021 gcggctggaa ccagcgattc gcgacgatcc cggtgacgcg gggcggggcg ccggtgccga
    10081 gcgaggcgtg ccccggcccc gtttcggtgt tcaggtgggc gtagtgcgcg ttcctctcga
    10141 cgtgcccttc gtcgaggagc tgcttcagcc cggcgacgaa gtagggccgg tagtcgag
//
LOCUS       DAOONL010000119        10121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7609_length_10121_cov_17.502782, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000119 DAOONL010000000
VERSION     DAOONL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_10121_cov_17.502782"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLP50_13880"
     CDS             complement(<1..526)
                     /locus_tag="PLP50_13880"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPA52679.1"
                     /translation="MTTPAGEPPRRSRLRRAAVTLLWLVLLAFVARHVAHAPAEVLGR
                     LSQARPWLLLAGGAAYALGFAARALRLNLLLPPASRISFGRAWAVSGATTFLLQVLPF
                     RGGEVASWVLYRRVLGTGWARAGALFVLVKTLDSAAFLLAGLAGAAALALRGGAPVLG
                     GVTAVAVAAGVVLLV"
     gene            complement(507..1481)
                     /locus_tag="PLP50_13885"
     CDS             complement(507..1481)
                     /locus_tag="PLP50_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA52680.1"
                     /translation="MKLVIQIPAWDEEEQIAAAVRELPRQVPGFARVEVLVVDDGSTD
                     ATARVAKEAGADHVLRLPIHRGLAVAWRAGLDRALALGADVIVSTDADRQYAAADVVT
                     LVGPILRGEAEIVVGDRGVATKEDFSPGKRRMQRFGSWVVRRASGTEVPDATSGFRAL
                     TREAALRLNVFGRMTYTVETLIQAGHKEMAVASVRVGTNPPVRPSRLFRRTSRYVLVQ
                     AANILRITALYKPLPVFLGLSALLFLLGTGLFGRYAWILVTDPTPGAHLQSLLLATVF
                     LVTSFLAFLTGLLADLISINRKLSEEILLRLRRREAAPGAEDRDDARG"
     gene            1562..2059
                     /locus_tag="PLP50_13890"
     CDS             1562..2059
                     /locus_tag="PLP50_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HPA52681.1"
                     /translation="MSTRRERFLFRIINVYPPYLGAGVRVLHREGDERTFRVEMRLRP
                     WNRNLFGTHFGGSLYAMCDPWFCWLLVRHLGSAYLVWDKAASIEFLKPGRGRVSATFH
                     VSSETVEEIRAAADRDGKTEPVLTAEVVADDGEVVARVTKTLWVRRKDAPKPPGRDVP
                     DPGSP"
     gene            2113..2778
                     /gene="rpe"
                     /locus_tag="PLP50_13895"
     CDS             2113..2778
                     /gene="rpe"
                     /locus_tag="PLP50_13895"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPA52682.1"
                     /translation="MAKLAPSILSADFGRLAAHAREAIAAGADWLHVDVMDGRFVPNL
                     TIGPLVVEALRPLREETGALLDVHLMIVEPERYLTDFARAGADVLTVHVEACPHLHRT
                     VQQIRELGVKAGVTLNPATPLSALEEILPDVDQVLVMSVNPGFGGQSFIPGATARVRR
                     LRAMLDGIGSTAELEIDGGIKPENAREVVDAGATVLVAGSAVFRGDVAANVRRLREAA
                     GPR"
     gene            complement(2913..3572)
                     /locus_tag="PLP50_13900"
     CDS             complement(2913..3572)
                     /locus_tag="PLP50_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA52683.1"
                     /translation="MTPVNPTRTSNLATLAAAIILLGGALGANAQPVLEITPTVGFRW
                     GGEIRAEETTTLDRDADLEEGHSLGLVIDIPVTEHLQIELLADHQRTGLDSGTLFGPS
                     DRQFDLDIDYYHVGALYQWTTARVKPFVVGSMGIANLDPRDGFNSSTRRFSGSLGGGV
                     KIPLSANIGVRFEGRAFWTDTGDTRFDDDEDDHHHGCHDDECSSGLFQAQARLGVTVS
                     F"
     gene            3757..4971
                     /locus_tag="PLP50_13905"
     CDS             3757..4971
                     /locus_tag="PLP50_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52684.1"
                     /translation="MKRGAGPHSSALRASLRRSAPLLALLAFALAGAPAARSEDAPRP
                     FVLTFSNPGARSLAFGGAFAPLADDATAAYANPAGLVQLLRPEVSVELRVSGALEDDD
                     ATGDPTVKRANGLSYFSAVYPLRRFSLALYGHQLAGIDIEPPGEEGPEAVARTARRAA
                     AGERLSGLDVWRLGVSAAFRLRDDLSVGLGASYFDGNVELAPSDDPDAAGRSVSSNDW
                     TLNTGVLWQPAEQLRLGAFYRQGPTLSLGRLGATPCAGRECVEASLRLPDTFGAGAAL
                     RSAGGGLTVAFEWDRVRYSSLVAGLSAAALGADRLSLSDANEFHVGAEYAFLRLDPVL
                     AVRVGGWLDPDHRLCGAAADSAYRCETDVRSGEGHVTLGFGMAFKRFQLDLAVDGSRA
                     EVTTSISGIFSF"
     gene            complement(4961..5959)
                     /locus_tag="PLP50_13910"
     CDS             complement(4961..5959)
                     /locus_tag="PLP50_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52685.1"
                     /translation="MNVPYVVGPWVRGERFYGRSDLLREALHGPRDRLWLVGMRRVGK
                     TSLLREVERLAGDGPGPFLPVVWDLQGSDEPDELDLGLHEALIDVEPALAAAGVGLPK
                     PGPASATLAGLAGELGRHGRRLLLLCDEAEDLFALLRRHPERLGSLESAWNESGTRVV
                     LASSVRLAGSSTSGPDHRLLAAFGAPLWIGPLEDEEARRLLRQENLPAGRRPDLAEAA
                     VDLLTTRCGGHPFLLQLGAKRAIELGNAQEACEGLARDPTVTRLCDVDLGLLGDADRE
                     LLAGIASPSAGDATCVAERIARLRALGLVRSGAGGRPEPGSWFLGRHLAAAARPRS"
     gene            complement(5956..8355)
                     /locus_tag="PLP50_13915"
     CDS             complement(5956..8355)
                     /locus_tag="PLP50_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52686.1"
                     /translation="MRARAPRPRPFAPALVAFAFVLLAAPARADRWYEHYARAEKALA
                     ASDWRTAVAELNGAIEAKGDSGVRERTYGMRVVDYFPYLRLGIAYFHLGEIDAARQAF
                     ETEERLGAVARSVEARVLLKRYRQLTDEARAGAEEGESERVAAIVARALSEADALEAQ
                     GKPTEALDALSRALAVSPEHAGAVTRAARLRKDADRRERERQESEHAAGLVSSARVAL
                     AAGRFAEAASLLRQALSLRPGADPEGLLRRAEEGMLSALDAARGSRAAAVDEALASAR
                     ELAGTGRNAEALDRLQPALALDPGNRDAAALLERLLSDRRSNAKRDAVARALAEADAE
                     HAAGRHERALAAANRALALDAASEPARAAVRRAYGEISRALLGRGTGQNLPPAIRFSD
                     QRETLPDGTRAEIVDAPEFRLSGVVFDRSAVRVHVGVGTDAGAPAGTAGQDVGDFVLT
                     EFSSTSRLAPGRTTLRVVATDAEGLSASAEYVVLYERPFARSPWALALLAAIPLAAAG
                     AFVTGRRLRRRRRRRRKFNPYVAGAPVLDERLFFGREPLIDRVLQTIPNNSLLLYGER
                     RIGKTSLLHHLKRRLEQLDDPEFAFHPVLVDLQGVPEERFFGALMVAILDELGPLLPP
                     GAVAPEAGRVAGYGYHELTRDLRAVLESLGRRTVKRVKLVLLIDEVDELNSYDPKVNQ
                     RLRSLFMRAFSEHLVAVVSGVGILKHWELAGSPWYNFFEELEVAPLDRGAAEALVRRP
                     IDGVFDIDDAALQRLVDVTAGRPYRIQKACVALVGRLHQEGRGTITLADVEAIAGEGL
                     E"
     gene            complement(8352..9764)
                     /locus_tag="PLP50_13920"
     CDS             complement(8352..9764)
                     /locus_tag="PLP50_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPA52687.1"
                     /translation="MASSERGPAVGVGSLELGGWLVQPDQNRISRPGASVQLEPRLMD
                     VLVFLADRAGQVVSRDQLVEGVWKVEFITEWAITRAIAKLRRALGDVAGQPRYIETIS
                     KRGYRLLAPVKAASPPVPPERNPATNARPAVAAMSRPFVAGGWVRAERFFGRDAQLAE
                     ILDGPRDALWVVGTRAVGKTSLLRQAEYLTAAAPERGFMPLVLDLEGVEDAAELEEDL
                     AEALLDASPRLSAAGIAPGEVPSGDFFTSLGRLRRLLGAQGLKLLLLVDEAEELLRLR
                     EREPAILRKLRRALQSREGIRTVLAASRRLWALADPDGETSPFLHGFTPPVYLGALAD
                     SEARDLLGPARPGPGAGMAFDARTVERIRETCGNHPFQLQALAARVLERGDTEAAIAE
                     LEDDPALGRLFSVDLSLLDEEDRDLLRGLAAADEAAARTSGPTGRLLELERLGLLRRA
                     PDGSVAIASHFFRRWLVTRS"
BASE COUNT         1383 a         3565 c         3874 g         1299 t
ORIGIN      
        1 ggacgaggag gacgaccccc gcggcgaccg ccacggccgt cacgccgccg aggacgggtg
       61 cccctccacg cagcgcgagc gccgcggccc cggcgagccc ggcgaggagg aacgccgcgc
      121 tgtcgagcgt cttcacgagg acgaagagcg cgcccgcgcg cgcccagccg gtgccgagga
      181 cgcgccggta gagcacccag ctcgcgacct cgccgccgcg gaacgggagg acctgaagga
      241 ggaacgtcgt cgcccccgag acggcccacg cgcggccaaa cgagatgcgg ctcgccggcg
      301 gaaggaggag gttcagccga agggcgcgcg ccgcgaagcc gagcgcgtag gcggcgccgc
      361 ccgccaggag gagccacggg cgcgcttgcg agaggcggcc gaggacctcc gccggggcgt
      421 gggcgacgtg acgcgccacg aacgcgagga ggacgagcca gaggagcgtg accgccgcgc
      481 ggcgcaggcg ggatcgccgg ggaggctcac ccgcgggcgt cgtcacggtc ctcggcgccg
      541 ggcgccgcct ccctgcggcg caggcgcagg aggatctcct ccgacagctt cctgttgatc
      601 gagatcaggt cggcgaggag acccgtcagg aaagccagga acgacgtgac gaggaagacg
      661 gtcgcgagga ggagcgactg caggtgcgcg ccgggcgtcg ggtcggtgac gaggatccac
      721 gcgtagcggc cgaagagacc ggtcccgagg agaaagagga gggcggagag gccgaggaag
      781 accgggagcg gcttgtagag cgcggtgatc cggaggatgt tcgcggcctg gacgaggacg
      841 tatcgcgacg tgcggcggaa gaggcgcgac gggcggacgg gcgggttcgt cccgacgcgg
      901 acggaggcga ccgccatctc cttgtgcccc gcctggatca gcgtctcgac cgtgtaggtc
      961 attcgtccga agacgttcag acggagcgcc gcctcgcgcg tcagggcgcg gaagccggac
     1021 gtggcgtcgg gcacctcggt gccggaggcg cgcctcacga cccacgaccc gaaccgctgc
     1081 atccgccgct tgccggggga gaagtcctct ttcgtcgcca ccccgcggtc gccgacgacg
     1141 atctccgcct ccccgcgcag gatcgggccg acgagggtca cgacgtcggc cgccgcgtac
     1201 tgccgatcgg cgtcggtcga gacgatcacg tcggctccga gcgccagcgc cctgtcgagc
     1261 ccggctcgcc aggcgaccgc gagcccgcga tggatcggaa gccggaggac gtggtcggcg
     1321 cccgcctcct tcgcgacgcg cgccgtcgcg tcggtcgacc cgtcgtcgac gacgagcacc
     1381 tcgacgcgag cgaaaccggg gacctgccgg gggagctcgc ggacggcggc cgcgatctgc
     1441 tcctcctcgt cccaggccgg gatctggatg acgagcttca cgacacccga ggatatccga
     1501 agccctgcgg cggacggggc gcggcgttcc gcctccgatg ccgatcgact atgttcgggg
     1561 catgtcgacg cgccgcgagc gcttcctctt ccggatcatc aacgtctacc cgccgtatct
     1621 cggcgcgggg gtgcgagtcc tgcaccgcga gggagacgag cggaccttcc gcgtcgagat
     1681 gcgcctgcgc ccgtggaacc ggaacctctt cggcacgcac ttcggcggct cgctctacgc
     1741 gatgtgcgac ccgtggttct gctggcttct cgtgcgccac ctcgggagcg cctacctcgt
     1801 ctgggacaag gccgcgtcga tcgagttcct caagccgggc cgtggtcgcg tctcggcgac
     1861 gttccacgtc tcgtcggaga cggtcgagga gatccgcgcc gcggccgacc gggacggcaa
     1921 gacggagccg gtcctgaccg cggaggtcgt cgccgacgac ggcgaggtcg tcgcgcgcgt
     1981 gacgaagacg ctctgggtga ggcgcaagga cgcgccgaag cctccggggc gggacgtccc
     2041 cgacccgggc tcgccctgag ggcgcgtgcg ggcgcggttc cccggcgagg cgatctcgga
     2101 gaagatcggt ccatggcgaa gctcgccccc tccatcctct ccgccgactt cggccggctc
     2161 gccgcccacg cccgcgaggc gatcgcggcc ggcgccgact ggctccacgt ggacgtcatg
     2221 gacgggcggt tcgtcccgaa cctcacgatc ggcccgctcg tcgtcgaggc gctccgcccg
     2281 ctgcgcgagg agaccggggc gctcctcgac gtccacctca tgatcgtgga gcccgagcgt
     2341 tacctcaccg acttcgcgag ggcgggagcc gacgtcctga cggtccacgt cgaggcgtgt
     2401 ccgcacctcc accggacggt gcagcagatc cgcgagctcg gcgtgaaggc gggcgtcacg
     2461 ctcaaccccg cgacgccgct ctccgccctc gaggagatcc tcccggatgt cgaccaggtg
     2521 ctcgtcatgt cggtcaaccc cggcttcggc ggccagtcct tcatccccgg cgcgaccgca
     2581 cgcgtccgcc gcctccgcgc gatgctcgac ggaatcgggt cgacggcgga gctggagatc
     2641 gacggcggca tcaagccgga gaacgcccgc gaggtcgtcg acgccggagc cacggtcctc
     2701 gtcgccggct ccgccgtgtt ccggggagac gtcgcggcga acgtgcggag gctccgggag
     2761 gcggcgggac cgcggtagga cgggagccgc gcgcggacgg ttccgccgac ggcctcgacg
     2821 aagcggtctg ccgaggcgcc cctccccgaa acgcatcggc cgccccgcgg tgcggggcgg
     2881 ccgggaaggg ggccggcggg gcagacggcg ggtcagaagg agacggtgac gccgaggcgg
     2941 gcctgggcct ggaagaggcc gctcgagcac tcgtcgtcgt ggcatccgtg gtggtggtcg
     3001 tcctcgtcgt cgtcgaaccg cgtgtcgccg gtgtcggtcc agaaggcgcg cccctcgaag
     3061 cggactccga tgttggcgga gagcgggatc ttcacgccgc cgccgagact cccggagaag
     3121 cgccgggtgc tggagttgaa cccgtcgcgt ggatcgaggt tggcgattcc catcgacccg
     3181 acgacgaagg gcttcacgcg ggcggtcgtc cactgataga gcgcgccgac gtggtagtag
     3241 tcgatgtcga ggtcgaactg gcggtcggac gggccgaaga gcgtgccgct gtcgaggccg
     3301 gtgcgctggt ggtcggcgag gagctcgatc tggaggtgct cggtgacggg gatgtcgatc
     3361 acgaggccga gcgagtgacc ttcctcgagg tcggcgtcgc gatccagcgt ggtcgtttcc
     3421 tcggcccgga tctcgccgcc ccagcggaag cccacggtcg gtgtgatctc gaggacgggc
     3481 tgggcgttcg ctccgagcgc tcctccgagc agaatgatcg ccgcggcgag ggtggcgagg
     3541 ttggaggtgc gggtcgggtt gaccggtgtc atggtcgatg gtcctccttg aatctctggg
     3601 ggccagggca tgtcgcccgg cacggtccac aagatccgcc ctgcggccgg cagacgcttg
     3661 accgaaccgt gatcaccgcg tgacctcggc gtgaggaatg cggacgaggc ctcgacgggc
     3721 ccgaaagccg cgttcttccg ggttagcatc ccggccgtga aacgcggcgc cggcccgcat
     3781 tcctccgctc tccgcgcatc gctccgccgt tccgcgccgc tgctcgcgct cctcgccttc
     3841 gccctggcgg gggcgcccgc ggcccgctcg gaggacgctc cgcggccgtt cgtcctgacc
     3901 ttctccaacc cgggcgcgcg cagcctcgcg ttcgggggcg ccttcgcgcc gctcgccgac
     3961 gacgccaccg ccgcgtacgc gaacccggcg gggctcgtgc agctgctccg gcccgaggtc
     4021 tccgtcgagc tgcgcgtcag cggcgctctc gaggacgacg acgcgaccgg ggaccccacg
     4081 gtgaagcggg ccaacgggct ctcgtacttc tcggcggtct acccgctgcg gcggttctcg
     4141 ctcgccctct acggccacca gctcgcgggc atcgacatcg agccccccgg cgaggagggc
     4201 ccggaggcgg tggcgcggac ggcccggcgg gcggcagcgg gggagcggct ctccggcctc
     4261 gacgtctggc gcctcggcgt ctctgcggcg ttccggctgc gcgacgacct cagcgtcggc
     4321 ctcggcgcgt cgtacttcga cgggaacgtg gagctggccc cgagcgatga cccggacgcc
     4381 gcagggcgga gcgtttccag caacgactgg acgctcaaca ccggggtgct ctggcagccc
     4441 gcggagcagc tccggctggg ggctttctac cgccagggac cgacgctctc gctcgggagg
     4501 ctcggcgcga cgccctgcgc gggacgggag tgcgtcgagg cctcgctccg cctccccgac
     4561 accttcggcg ccggcgccgc gctgcggtcg gccggcggcg ggctcacggt ggccttcgag
     4621 tgggaccggg tccgctactc gagcctcgtc gcggggctct ccgcagcggc gctcggcgcc
     4681 gaccggctct ccctctcgga cgcgaacgag ttccacgtgg gcgccgagta cgcgttcctg
     4741 cggctcgacc cggtgctggc cgtgcgcgtc ggcgggtggc tcgaccccga tcaccgcctt
     4801 tgcggcgcgg ccgcggactc ggcgtaccga tgcgagaccg atgtccgctc gggcgagggg
     4861 cacgtcacgc tgggcttcgg aatggcgttc aagcgcttcc agctcgacct ggcggtcgac
     4921 ggctctcgcg ccgaggtgac gacgtcgatc tccgggatct tcagcttcta ggccgcgctg
     4981 ccgccgcgag gtggcgaccg aggaaccagc tgccgggctc cgggcgcccg ccggcgccgc
     5041 tccgtacgag gcccagcgcg cgaaggcggg cgattcgctc ggcgacacag gtcgcgtcgc
     5101 ccgccgaagg ggaggcgatt ccggcgagaa gctcgcgatc ggcgtccccg aggaggccga
     5161 gatcgacgtc gcagaggcgt gtcaccgtcg ggtcgcgggc cagcccctcg cacgcttcct
     5221 gggcgttgcc gagctcgatc gcgcgcttgg cgccgagctg gaggaggaac ggatgcccgc
     5281 cgcaacgagt cgtcaggaga tcgaccgcgg cttcggcgag gtcgggcctc cgccccgcgg
     5341 gtaggttctc ctgcctcagg aggcggcgcg cctcctcgtc ctccaggggt ccgatccaca
     5401 acggtgcgcc gaaggccgcg agaaggcggt ggtccggtcc gcttgtcgaa gacccggcga
     5461 ggcggacgga ggaagccagg acgaccctcg taccggactc gttccacgcg ctttcgagcg
     5521 atccgagccg ctccgggtgg cgccgcagga gggcgaagag gtcctcggcc tcgtcacaga
     5581 ggagaagcag ccgccggccg tgtcgtccga gctccccggc gaggccggcg agcgtcgccg
     5641 acgccggccc gggcttcggc agcccgacgc ccgccgcggc gagagcgggt tcgacgtcga
     5701 tcagcgcctc gtggaggccg aggtccagct cgtccggctc gtccgagccc tggaggtccc
     5761 agacgactgg caggaagggc cccggcccgt cgccggcgag ccgttcgacc tcgcggagga
     5821 gcgaggtctt gccgacgcgg cgcatcccga cgagccagag ccgatcccgt ggcccgtgga
     5881 gcgcctcccg gagcaggtcg ctccggccgt agaagcgctc gccgcgcacc cacgggccga
     5941 cgacgtacgg gacgttcatt cgaggccctc gccggcgatc gcctccacgt cggcgagcgt
     6001 gatcgtcccg cggccctcct ggtggagccg gccgacgagc gcgacgcacg ccttctggat
     6061 ccggtacggc cggccggcgg tgacgtcgac cagacgctgg agggccgcgt cgtcgatgtc
     6121 gaagacgccg tcgatggggc ggcgcacgag cgcctcggcg gccccgcggt cgagcggcgc
     6181 gacctcgagc tcctcgaaga agttgtacca gggcgagccg gcgagctccc agtgcttcag
     6241 gatcccgacc ccggagacga cggccacgag gtgctcggag aaggcgcgca tgaagaggct
     6301 tcgaagcctc tggttcacct tcgggtcgta ggagttgagc tcgtcgacct cgtcgatgag
     6361 aaggacgagc ttcacgcgct tgaccgtgcg gcgtcccagg gattcgagca cggctcgcag
     6421 gtcgcgggtg agctcgtggt agccgtagcc ggcgacgcgg cccgcctcgg gggcgacggc
     6481 gccgggcggg aggagcgggc cgagctcgtc gaggatcgcg accatcaggg caccgaagaa
     6541 gcgctcctcc gggaccccct ggaggtcgac gaggacgggg tggaacgcga actccgggtc
     6601 gtcgagctgc tcgagccggc gcttgaggtg gtggaggagc gaggtcttgc cgatgcggcg
     6661 ctcgccgtag aggaggaggc tgttgttggg aatcgtctgc aggacccggt cgatgagcgg
     6721 ctcgcgtccg aagaagagac gctcgtcgag gactggcgcg ccggcgacgt agggattgaa
     6781 cttccgtcgc cgccggcgtc gcctccggag ccttcggccg gtgacgaagg cacccgccgc
     6841 ggcgagcggg atcgcggcga ggagcgcgag ggcccacggc gagcgcgcga agggccgctc
     6901 gtagaggacg acgtactcgg cgcttgccga gaggccctcc gcgtcggtgg cgacgacgcg
     6961 gagcgtcgtg cggcccgggg cgagccgcga cgtcgacgag aactccgtga ggacgaagtc
     7021 gccgacgtcc tggcccgcgg tgccggcggg agcaccggcg tccgtcccga cgccgacgtg
     7081 gacgcggacg gccgaccggt cgaacaccac ccccgagagg cggaactccg gggcgtcgac
     7141 gatctccgcc cgcgtgccgt ccggaagggt ctctcgctgg tccgagaaac ggatggccgg
     7201 agggaggttc tgaccggtgc ccctgccgag gagcgcgcgg ctgatctcgc cgtaggcgcg
     7261 acggacggcg gcgcgggccg gctcgctcgc agcgtcgagg gcgagggcac ggttcgcggc
     7321 ggcaagcgcc cgttcgtggc gccccgcggc gtgctcggcg tccgcttcgg cgagggcgcg
     7381 cgccacggcg tcccgcttcg cgttggagcg gcgatcggag aggagacgct cgaggagcgc
     7441 cgccgcgtcg cggttgccgg ggtcgagcgc cagcgccggc tggaggcgat cgagcgcctc
     7501 ggcgttgcgc ccggtaccgg ccagctcgcg ggccgatgcg agcgcctcgt cgaccgcggc
     7561 ggcacgcgac cctcgggcgg cgtcgagggc cgagagcatt ccctcctccg cacggcgcag
     7621 gagcccctcc gggtcggcgc ccggccggag cgagagggcc tgtctcagga ggctcgccgc
     7681 ctccgcgaaa cgcccggcgg ccagggcgac acgggccgag gagacgagac ccgcggcgtg
     7741 ctcgctctcc tgcctctcgc gctcgcggcg gtcggcgtcc tttcggagcc gggccgccct
     7801 cgtgacggct ccagcgtgct cgggagagac ggcgagcgcg cgcgagagcg cgtcgagcgc
     7861 ctccgtgggc ttcccctgag cctcgagcgc gtcagcttcg ctcagggccc gcgcgacgat
     7921 cgccgccacc cgctccgact cgccctcctc ggcgcccgcg cgggcctcgt ccgtcagctg
     7981 ccggtaccgc ttgaggagca cgcgggcctc gacggagcgc gcgacggcgc cgagccgttc
     8041 ctcggtttcg aacgcctgcc gtgcggcgtc gatctctccg aggtggaagt aggcgatgcc
     8101 cagcctgagg taggggaagt agtcgacgac ccgcatgccg tacgtgcgct cgcggacgcc
     8161 ggaatcgccc ttcgcttcga tcgcgccgtt cagctcggcc acggccgttc gccagtcgct
     8221 ggcggcgagc gccttctcgg cgcgcgcgta gtgctcgtac cagcggtccg cgcgggcggg
     8281 cgcggcgagg agaacgaagg cgaaggcgac gagggccggc gcgaacgggc gcgggcgggg
     8341 tgcgcgcgct ctcacgaccg ggtcaccagc cagcggcgga agaagtggct cgcgatcgcg
     8401 acggagccgt ccggcgcgcg acggaggagg ccgagccgct cgagctcgag gagccggccc
     8461 gtcgggccgg atgtccgcgc cgcggcctcg tccgccgcgg ccagaccgcg caggaggtca
     8521 cggtcctcct cgtcgaggag cgagaggtcg accgaaaaga ggcgcccgag cgccgggtcg
     8581 tcctcgagct cggcgatggc cgcctccgtg tcgccgcgct cgaggacgcg cgccgcgagc
     8641 gcctgcagct ggaacgggtg gttcccgcag gtctcgcgga tcctctccac cgtcctcgcg
     8701 tcgaacgcca tcccggcgcc ggggcccggg cgggccgggc cgagcaggtc gcgcgcctcg
     8761 gagtcggcga gcgcgccgag gtagacgggc ggcgtgaagc cgtgcaggaa aggcgacgtc
     8821 tcgccgtccg gatcggcgag cgcccagagc cggcgcgagg ccgccagaac ggtgcggatc
     8881 ccttcgcgcg actggagcgc gcggcggagc ttgcgcagga tcgcgggctc gcgctcccgc
     8941 agccggagga gctcctcggc ctcgtcgacg aggagcagca gcttgagtcc ctgggcgccg
     9001 agcaggcggc gcaggcgtcc gagcgacgtg aagaagtcgc cggacgggac ctcgccgggc
     9061 gcgatccccg cggccgagag gcgcggagag gcgtcgagga gcgcttccgc gaggtcctcc
     9121 tcgagctcgg cggcatcctc gactccctcg aggtcgagaa cgagcggcat gaagccgcgc
     9181 tccggtgcgg cggccgtcag gtattcggcc tgtcggagga gcgaggtctt gcccacggcc
     9241 cgggtcccca cgacccagag cgcgtcgcgc gggccgtcga ggatctccgc gagctgcgcg
     9301 tcccgcccga agaaacgctc ggcgcggacc caccccccgg caacgaaggg tctcgacatg
     9361 gcggcgaccg ccgggcgcgc gtttgtagcc gggtttcgtt cgggcggcac cggtggcgac
     9421 gccgccttca ccggggcgag caggcgatag cctcgcttcg agatcgtctc gatgtagcgt
     9481 ggctggccgg ccacgtcgcc gagagcccgc cggagcttcg cgatcgcccg ggtgatcgcc
     9541 cactcggtga tgaactcgac cttccagacg ccttcgacga gctggtcgcg ggagacgacc
     9601 tggccggccc ggtcggccag gaagacgagg acgtccatga gccgcggctc gagctgcacg
     9661 ctcgcgcccg gacgcgagat ccgattctgg tcgggctgga cgagccagcc acccagctcg
     9721 aggctgccga ccccgacggc gggtccgcgc tcggaactgg ccatcggtgc cgattctagc
     9781 ggtgcgggga ggaggccctc ggaggcgagg catcggtccc ggcccgccgc tcgcgcggga
     9841 gcgaggcgtt ccatgcggcc acgctaggac acccacgcgg ggaaagggtg accgatcagg
     9901 aaccgaacga tgacccccga tcacgtcccg gcgacggcga cgaggacgcg gggcggccaa
     9961 aggaggccgg gaggcggaaa atgcgggcga ttcgggtctt ccggcgcggg ccgaccgccg
    10021 catccggcgc ccccccgctt gacggctccg ggcaggtccc cttattctcc gcggtcccgg
    10081 cacgtgtgcc ggagcgggtc ttttgagaat cagatccggg t
//
LOCUS       DAOONL010000120        10107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7621_length_10107_cov_19.010545, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000120 DAOONL010000000
VERSION     DAOONL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_10107_cov_19.010545"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLP50_13925"
     CDS             <1..324
                     /locus_tag="PLP50_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52688.1"
                     /translation="VLLENLIGNALKFTSSRPEATIEVGCTERDGERVFFVKDDGVGF
                     DMAYASKLFLPFQRLHSASDFPGTGIGLATVQRIVARHGGRIWPESAPDAGATFFFTL
                     GGGGG"
     gene            complement(346..5142)
                     /locus_tag="PLP50_13930"
     CDS             complement(346..5142)
                     /locus_tag="PLP50_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPA52689.1"
                     /translation="MTPPDRVPTSPRPAAEPPRGSAVLTVSGMTCASCARLIEWKVGQ
                     LAGVRSARVSVATDALHVEFDAARVDVGRVAACVRRAGFGTPGVGTGEDDGDPGGERP
                     GPARARLVLGLVLTGPLIVYSMGRDLGLPAFPYDQLVLLLAATIVQLVVGAPFYVRSW
                     RSLRAGASSMDVLVALGSTVAWGASAGVVLGVLPGSDVYFETGAAIVTFVSLGRYLEA
                     RVRGRASRALRGLLDLSPVRARVVRDGVEVEVGAADVRVGETVVVRPGERVPVDGLVR
                     EGRSAFDESMVTGESMPVVKGPGDEVIGATVNRDGLVRVEATRVGADATLARIVRLVE
                     EAQARRAPIQKVADAVGRVFVPLVLASALGTLLLWLFVARIDVPGALMNAVAVLIIAC
                     PCAIGLATPMAVLVGTTRGASRGLLFRTGEALERVGAATVVVLDKTGTLTRGEPGLTD
                     VVAVRRGEESEVLRLAASAELGSEHPAGRALARAGEARGLSLSAPRELRAVSGAGIRA
                     IVDGREVLVGTLWLMREAGVPLEAVAPEVDRLQAEGKTAMVVAAAPPEGGAPEAIGVL
                     AVADAVKPEAREAVEELASLGLDVVMLTGDNLRTAEAIAREVGIERVRAEVRPEEKAE
                     AIRRLREAPRAPGTPPPVVVMVGDGINDAPALAQADVGIALGTGTDVAKEAAAVTLMS
                     GDLRGVGRAIVLSRASLQTVHQNLVWALFYNVALIPVAAYGLLSPMVAAGAMVFSSLF
                     VVTNSLRLRKAPLEAFVAPREAWRRGLALVPRVLAPAAALAALIVVPMLAMADGAEIR
                     GALPGTMTPALMMVMAIANGVIAVSYASIPVFLIVFKVRRKDLPFSWVLVLFGLFILA
                     CGTTHFVHVLGLWWPVDWWQATVDSICAAASLATAVVLWPLLPRILALPSPEQLRLVN
                     RALEDEKASLERTRDELRRAYAEVERRVVERTAELAAANAALVAEVAVREAAEEELRR
                     SRNMLAHVMDAIPQSVFWKDRESVYMGCNRQMASRAGLKDPAGIVGLTDFDLPWTRSE
                     SEAYRADDREVIASGRPKLHILESQRMPDGSATWLDTSKVPLTNGGGEVYGVLGIFED
                     VTDRREAETRLHEYRKVIESSTDLICVLDDVRRFVLANGALLSFRGASGERVAGRGVG
                     EVLGEEALARLGPELDACFAGKEREFGMTMESPGRGLRDLSVLVIPVQEGDVVTRAAC
                     VFRDVSDQRKLESQLLQAQKMEAIGQLAGGVAHDFNNILSVILGYCHLLGPALSADAK
                     QAERLGQVVAAAERASQLTRALLAFSRKQAMSPKALNLHDVVLRVQKLLLRIIGEDVR
                     LRTVTNDATLVVFADGGQLEQVLINLATNARDAMPKGGVLTIETGVQVLDERFSALHG
                     WGSPGRYAVLSVADTGCGMDEETRKRIFEPFFTTKEAGKGTGLGMSIVYGIVKQHRGF
                     LSVYSEPGGGSVIRVYLPIHEAGSAMDDEVATSPPVRGGSETILVAEDEASVRHLLLT
                     VLAEAGYDVVLAEDGQDAIDKFAARRDGIHLVLMDLIMPRKGGREAADEIRRLRPGVK
                     LLFSSGYSPDLAEVRGAGRESFELVMKPVAPLDLLRRVREVLDRPS"
     gene            complement(5165..5608)
                     /locus_tag="PLP50_13935"
     CDS             complement(5165..5608)
                     /locus_tag="PLP50_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA52690.1"
                     /translation="MSDRRGLVRPGAPIERVLLVDDEEPLVELLTLSLGDLGYRVTGH
                     TDARAALEAFLKEPDAFDALVSDLSMPHLAGFELAERLLRVRPEFPVVLTSGWVREED
                     ETAGRAIGILGFAAKGSSVEEIVRAIDALLRGGTSPSTPQGAEAG"
     gene            complement(5820..7157)
                     /locus_tag="PLP50_13940"
     CDS             complement(5820..7157)
                     /locus_tag="PLP50_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA52691.1"
                     /translation="MSEALPAADPRPLPPTAYRWLVLLSCSVAMFGNYYVFDALYPVT
                     PLLEKAFGFTGAQVGLLDTAYNVAALLTLIAGGILIDRMGVAASAVLFGAIGAAGSIA
                     IAVLPALLPDTPWVGMMLGRFLLGVGSECFIVAATTVVGRWFKGKEISFALAIQLLVG
                     RLGSWAADKSPDFAKSLFETWQPPLLLAAVLGVVWLVFALIYAGLEKRAARVYAVKKA
                     GATDKLVWKDLVRFDLGYWWVVGLCVAFYSTIFPFRTFANLYFIEAKGLAPEDAGNLK
                     SILPLLSMIGMPLFGLLVDKIGKRALLMAAGSALLVPPFFLLAFSSAPPTVLMAMLGL
                     AFALVPAVLWPAVTYLVPEARLGSAYALMTFCQQVGWAGMSWGLGLLKDASGASAAHP
                     AGWAPVMVALGALASVGFVFSFLLWRSERGAKGHGLDAVTPTAATESKPIEPA"
     gene            complement(7213..7470)
                     /locus_tag="PLP50_13945"
     CDS             complement(7213..7470)
                     /locus_tag="PLP50_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52692.1"
                     /translation="MSDTLLHEVLDVLQRRGTPAALIGAVAPAAHGIVRATLDYDLLD
                     AETLLDGEEAGRLRAEVESRLEELPEGLRREVSALLARRTG"
     gene            complement(7467..7691)
                     /locus_tag="PLP50_13950"
     CDS             complement(7467..7691)
                     /locus_tag="PLP50_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52693.1"
                     /translation="MVSDAFRRDAATNASRPTRGRTAEQRIAEALRMGEACLDVYLAA
                     LPAGTTREEARARAARIKRRGRRRSDASLS"
     gene            7759..9198
                     /gene="sthA"
                     /locus_tag="PLP50_13955"
     CDS             7759..9198
                     /gene="sthA"
                     /locus_tag="PLP50_13955"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HPA52694.1"
                     /translation="MTPFNSASDFDVVVLGGGPGGERAAIQAAKARKRVALIEKAPVV
                     GGARVNWGTIPSKTLRESALFFLGMTRHRLHGFQAEVTDDVTVADFMYRERLVVQREL
                     DMINASLGRYRIEIFRGHGRFLDPHTIAVEGKDGRRKGSVRGEVVVIATGSSPNRPAD
                     VPFDAESVFDSNTILKLPRMPKTMTVLGAGVIGIEYASIFAALGVYVTLVDTRDRLLP
                     YLDREVVGILERELGELHVELLHDDRYATVEPVPGSPARVTIETRKGRRLTSDVLLYC
                     VGRDGNTKDLGLEAIGLVPDKYGLLSVNADLQTTHPHIYAVGDVIGYPALASTSMEQG
                     RRAMRHAFGLTGPSASTENLPFAIYSIPEVSYVGETEEKLKEKGIDYVVGRGHYGMNP
                     RGQIIGDQDGLLKLLFDAKSGRLIGTHIVGHSASELVHVGQAYMKMQATAPEIAESLY
                     NYPTLSDMYRHAALTAVTELAKRRGTDAG"
     gene            9209..>10107
                     /locus_tag="PLP50_13960"
     CDS             9209..>10107
                     /locus_tag="PLP50_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HPA52695.1"
                     /translation="MASIEQLLGTVSGFLWGWPLIVLLFGTHLFLTLRLGFPQRHLLK
                     AIRISLRREKEGAGDVSQFGALTTALAATIGTGNIVGVSTAIAAGGPGAVLWMWLTGV
                     FGIATKYSEALLSVKYRVTDPDGTMSGGPMYVLERGLKMKWLGVLFAAFTAVAAFGIG
                     NTVQANSIAVLAKETFGVSPWVSGGLLTALTAVVILGGIKSIARVCELLVPFMAGSYV
                     LGCLYLLALHSETLPDAFRLIVSSAFTGQAAIGGFLGAGMKEAIRFGVARGLFSNESG
                     LGSAPIVAAAAMTKNPVRQALVSS"
BASE COUNT         1512 a         3746 c         3506 g         1343 t
ORIGIN      
        1 gtcctcctcg agaacctgat cgggaacgcc ctgaagttca cctcctcgcg tccggaggcg
       61 acgatcgagg tcgggtgcac cgagcgggac ggggagaggg tcttcttcgt gaaggacgac
      121 ggggtcgggt tcgacatggc gtacgcgtcg aagctcttcc tcccgttcca gcgcctccac
      181 agcgcgagcg acttcccggg aacgggaatc ggcctggcga ccgtccaacg gatcgtcgcg
      241 cgccacggcg gacggatctg gcccgagtcg gcccccgacg ccggtgcgac gttcttcttc
      301 acgctcggcg gcggcggagg ctgacgcggc ggagccggcc gcctgctagg acggccggtc
      361 gagaacctcg cggactcgcc gcaggaggtc caggggtgcg acgggcttca tgacgagctc
      421 gaagctctcg cggcccgctc cccggacctc ggcgaggtcg ggcgagtagc cgctcgagaa
      481 aagcagcttc acgcccggcc gaagcctgcg gatctcgtcc gcggcctccc ggcccccctt
      541 cctcggcatg atcaggtcca tgaggacgag gtggatgccg tcacgccggg ccgcgaactt
      601 atcgatcgcg tcctggccgt cctcggccag gacgacgtcg taccccgcct cggccaggac
      661 cgtgaggaga aggtgccgga cgctcgcctc gtcctccgcg acgaggatcg tctcgctccc
      721 gccgcggacg ggcggagacg tcgccacctc gtcgtccatc gccgagccgg cctcgtggat
      781 cgggaggtag acccggatca cgcttccacc gccgggctcg ctgtagacgc tgaggaagcc
      841 ccggtgctgc ttcacgatcc cgtagacgat cgacatcccg aggccggtcc ccttccccgc
      901 ctccttcgtc gtgaagaacg gctcgaagat cctcttgcgc gtctcctcgt ccatgccgca
      961 gcccgtgtcg gcgaccgaga ggacggcgta tcttcccggg gagccccagc cgtggagcgc
     1021 cgagaaccgc tcgtcgagca cctggactcc cgtctcgatc gtcaggacgc cgcccttcgg
     1081 catcgcgtcc cgcgcgttcg tcgcgaggtt gatcagcacc tgctcgagct ggccaccgtc
     1141 ggcgaagacg acgagcgtcg cgtcgttcgt caccgtccgg agcctcacgt cctctccgat
     1201 gatccggagg aggagcttct gcacccggag gacgacgtca tggaggttca gggccttcgg
     1261 ggacatggcc tgcttgcggc tgaacgcgag aagggcccgc gtcagctgag aggccctctc
     1321 cgcggccgcc acgacctgtc ccagccgctc ggcctgcttc gcgtcggccg agagcgcggg
     1381 gcccaggagg tggcagtagc ccaggatcac ggagaggatg ttgttgaagt cgtgggcgac
     1441 gccccccgcg agctgcccga tcgcttccat cttctgggcc tggaggagct gggactcgag
     1501 cttccgttgg tccgaaacgt cccggaagac gcaggccgct cgcgttacga cgtccccctc
     1561 ttggaccggg atgacgagga ccgacaggtc gcgaagccct ctccccgggg actccatcgt
     1621 catcccgaac tcccgctcct tcccggcgaa gcacgcgtcc agctccggcc cgagcctcgc
     1681 gagagcctcc tccccgagca cctccccgac cccgcgtccc gcgaccctct cgccggacgc
     1741 gccgcggaac gagaggaggg cgccgttggc gaggacgaac cggcggacgt cgtcgaggac
     1801 gcagatcagg tccgtcgagc tctcgatcac cttgcggtac tcgtggagcc gcgtctcggc
     1861 ctcccgccgg tccgtcacgt cctcgaagat cccgaggacg ccgtagacct cgcccccgcc
     1921 gttcgtgagg gggaccttcg acgtgtcgag ccatgtggcc gagccgtccg gcattcgctg
     1981 ggactcgagg atgtggagct tcggtcgccc ggaggcgatg acctcccggt cgtcggcccg
     2041 gtaggcctcg gactcgctcc gcgtccaggg gaggtcgaag tccgtcaggc ccacgatccc
     2101 ggccgggtcc ttcaggccgg ctcgtgaggc catttgccgg ttgcagccca tgtagacgct
     2161 ctccctgtcc ttccagaaga ccgactgcgg gatcgcgtcc atcacgtgcg cgagcatgtt
     2221 gcggctccgg cgcagctcct cctccgccgc ctcccggacc gccacctccg cgacgagggc
     2281 ggcgttcgcc gcggccagct cggccgtccg ctccacgacg cgccgctcca cctccgcata
     2341 cgccctcctc agctcgtccc gcgtcctctc gagcgaagcc ttctcgtcct cgagcgcccg
     2401 gttcacgagg cgaagctgct ccgggctcgg aagcgcgagg atcctcggga ggagcggcca
     2461 gaggacgacc gcggtcgcca gcgacgcggc ggcgcagatc gagtcgaccg tcgcctgcca
     2521 ccagtccacc ggccaccaga ggccgaggac gtggacgaaa tgcgtcgtgc cgcacgcgag
     2581 gatgaagagg ccgaacagga cgaggaccca cgagaacgga aggtccttcc ttcggacctt
     2641 gaagacgatg aggaagaccg ggatcgacgc ataggagacg gcgatcacgc cgttggcgat
     2701 cgccatgacc atcatcagcg ccggcgtcat cgtcccgggg agggcccctc ggatctcggc
     2761 cccgtccgcc atcgcgagca tcgggacgac gatcagcgcc gcgagcgccg ccgccggcgc
     2821 gaggacgcgc ggcacgagcg cgaggccccg ccgccacgcc tctctcggcg cgacgaacgc
     2881 ctcgagcggc gccttccgga ggcgcaggct gttcgtgacg acgaaaagcg agctgaagac
     2941 catcgctccg gccgccacca tcggactcag gaggccgtag gccgcgacgg ggatcagcgc
     3001 gacgttgtag aagagcgccc agacgaggtt ctggtggacc gtctggaggg acgcgcgaga
     3061 gaggacgatc gcccgtccga cgccccgcag gtcgccgctc atcagcgtga cggcggccgc
     3121 ctccttggcg acgtccgtcc cggtcccgag ggcaatgccg acatcggcct gagcgagagc
     3181 cggcgcgtcg ttgatcccgt cgccgaccat cacgacgacg ggcgggggcg tgccgggcgc
     3241 cctcggcgcc tcgcggagcc tccggatcgc ctccgccttc tcctccggcc tcacctccgc
     3301 gcgcacccgc tcgatgccga cctcgcgggc gatcgcctcg gcggtccgga ggttgtctcc
     3361 cgtcagcatg acgacgtcga gcccgaggga agcgagctct tccacggcct cgcgggcctc
     3421 gggcttgacg gcgtcggcga cggccaggac gccgatcgcc tccggcgcgc ccccctccgg
     3481 cggcgccgcg gccacgacca tcgcggtctt tccctcagcc tggagtcggt cgacctcggg
     3541 cgccaccgcc tccagcggca ctcccgcctc gcgcatgagc cagagcgtcc cgacgaggac
     3601 ctcgcggccg tccacgatcg cgcggatccc cgcgccgctc accgcccgaa gctcgcgggg
     3661 cgcggagaga gagagtcctc gcgcctcgcc cgctcgcgcc agcgcccggc cggccgggtg
     3721 ctccgacccc agctcggcgc tcgccgcgag ccggaggacc tccgactcct cgccccgtct
     3781 cacggcgacg acgtcggtga gacccggctc gccgcgggtg agggtgccgg tcttgtcgag
     3841 gacgacgacc gtcgccgccc cgacccgctc gagcgcctcg cccgtccgga agaggaggcc
     3901 tcgggaggcg cctcgcgtcg tcccgacgag gacggccatc ggcgtcgcga ggccgatcgc
     3961 gcacgggcag gcgatgatga ggacggcgac ggcgttcatc agcgcccccg gcacgtcgat
     4021 ccgcgcgacg aagagccaga ggaggagcgt cccgagcgcc gacgccagga cgagcgggac
     4081 gaacacgcgg ccgacggcgt cggcgacctt ctggatcggg gctctccgcg cctgcgcctc
     4141 ctccacgagg cggacgatcc gggcgagcgt cgcgtcggcc ccgacccgcg tcgcctcgac
     4201 ccgcacgagg ccgtcgcggt tgacggtagc gccgatcacc tcgtcgcccg gccccttcac
     4261 cacgggcatc gactcacccg tgaccatcga ctcgtcgaac gcggagcgcc cttcgcggac
     4321 gagaccgtcg accggcaccc gctcgccggg tcggaccacg acggtctcgc cgacccggac
     4381 gtccgccgcg ccgacctcga cctcgacacc gtcccggacg acgcgagccc ggaccgggct
     4441 caggtcgaga agccctcgga gggcccgcga cgcccgcccg cggacccgcg cctccaggta
     4501 ccggccgagc gagacgaacg tgacgatcgc ggctcccgtc tcgaagtaga cgtcggagcc
     4561 cggcaggacc ccgagaacga cgcccgcgct cgccccccac gcgacggtgg agccgagcgc
     4621 gacgaggacg tccatgctcg aggcgccggc ccggaggctc cgccaggagc ggacgtagaa
     4681 cggcgctccg acgacgagct ggacgatcgt cgcggccagg agcaggacga gctggtcata
     4741 cgggaaggcc ggaaggccga ggtcgcggcc catgctgtag acgatcagcg ggccggtcag
     4801 gacgaggccg aggacgaggc gcgcccgcgc gggaccgggg cgctcgcccc ccgggtctcc
     4861 gtcgtcctcg ccggttccga cgccgggcgt gccgaagccc gccctcctca cgcacgcggc
     4921 gacccggccg acgtcgaccc gggcggcgtc gaactcgacg tgcagcgcgt cggtcgcgac
     4981 gctgacgcgc gcggagcgga cccccgcgag ctgccccacc ttccactcga tcagccgggc
     5041 gcagctcgcg caggtcatcc ccgagacggt gaggacggcg ctcccgcgag gcggctcggc
     5101 ggcgggtcga ggcgaggtcg ggacgcggtc gggcggcgtc atcgttctcc tctcccggcg
     5161 agcgtcagcc cgcctcggcg ccctgaggcg tgctcgggct cgtaccgccc cgaagaaggg
     5221 cgtcgatcgc gcggacgatc tcctcgacgg aggatccctt ggccgcgaag ccgaggatcc
     5281 cgatggcgcg tccggcggtc tcgtcctcct cccggaccca tccggacgtc aggacgaccg
     5341 ggaactccgg acgcacgcga aggagacgct ccgcgagctc gaatcccgcg aggtggggca
     5401 tcgagaggtc ggagacgagg gcgtcgaacg cgtccggctc cttgaggaac gcctcgagcg
     5461 ccgcacgcgc gtcggtatgg cccgtgaccc ggtaccccag gtcgcccagg gagagcgtca
     5521 ggagctccac gagaggctcc tcgtcgtcca cgagaaggac ccgctcgatc ggcgctcccg
     5581 gccgtacgag tccccgccgg tcgctcatgc cccgtctctc gcggcttccc acacgaggga
     5641 accgcccggc tccgccaggc cgggaccgcc gtccccgtcc atcacgtcgg cattctccct
     5701 cccaccgcca ggattgtctc ctgcggcggg ctccgggcga caccgtttcg gcgaagcgcc
     5761 gagccgggag cccacgcgca ctcgccgggc ggagcgaccc gccggcgtgc ccggcccggt
     5821 caggccggct cgatgggctt cgattcggtc gccgccgtcg gcgtcacggc gtccagaccg
     5881 tgccccttcg cgccccgttc gctcctccag agaaggaacg agaagacgaa gccgactgag
     5941 gcgagggcgc cgagcgcaac catcacgggc gcccagccgg cggggtgcgc ggcgctcgcg
     6001 ccggacgcat ccttcaggag gccgaggccc cagctcattc cggcccagcc gacctgctgg
     6061 cagaacgtca tcagcgcgta ggccgagccg agtcgcgcct cggggacgag gtaggtcacc
     6121 gccggccaga ggacggccgg gacgagcgcg aaggcgagcc cgagcatcgc catcaggacc
     6181 gtcggcgggg ccgaggagaa cgcgaggagg aagaacggcg ggacgaggag cgccgacccg
     6241 gccgccatca ggagcgcccg cttcccgatc ttgtcgacga ggaggccgaa gagcggcatc
     6301 ccgatcatcg agaggagcgg gaggatcgac ttcaggttcc ccgcgtcctc gggcgcgagc
     6361 cccttcgcct cgatgaagta gaggttcgcg aaggtccgga acgggaagat cgtcgagtag
     6421 aacgcgacgc agaggccgac gacccaccag tagccgaggt cgaaacggac gaggtccttc
     6481 cagacgagct tgtcggtcgc gccggccttc ttcacggcgt agacgcgggc cgcccgcttc
     6541 tcgaggccgg cgtagatcag ggcgaagacg agccagacga ctccgaggac ggccgcgaga
     6601 aggaggggcg gctgccaggt ctcgaagagc gacttcgcga agtcgggcga cttgtcggcg
     6661 gcccacgagc cgaggcgccc gacgaggagc tggatcgcga gggcgaagga gatctccttc
     6721 cccttgaacc agcggccgac gaccgtcgtg gccgcgacga tgaagcactc cgagccgacg
     6781 ccgaggagga accggcccag catcatcccc acccacggcg tgtcgggaag gagcgcgggg
     6841 aggacggcga tcgcgatgct ccccgcggcc ccgatcgccc cgaagaggac ggccgacgcg
     6901 gcgaccccca tccggtcgat caggattccg ccggcgatca gcgtcaggag cgccgcgacg
     6961 ttgtaggccg tgtcgaggag tccgacctgc gcgcccgtga aaccgaacgc cttctcgagg
     7021 agcggcgtga ccgggtagag ggcgtcgaag acgtagtagt tgccgaacat cgcgacgctg
     7081 cacgagagga ggacgagcca gcggtaggcg gtcggcggca gggggcgcgg gtcggcggcc
     7141 gggagggctt cggacatgcg gggcggacga tactccttcc ccgcgtcggg gagcggcgag
     7201 gcccgtgtcc cgtcagccgg tccggcgggc gaggagagcc gagacctccc ggcggagacc
     7261 ttcgggcagc tcctcgagcc gagactcgac ctccgcgcga agacggcccg cctcctcccc
     7321 gtcgagcagg gtttcggcgt cgaggaggtc gtaatcgagc gtcgcacgga cgatgccgtg
     7381 ggccgcgggg gcgaccgcgc cgatgagcgc cgccggcgtg ccccgtcgct ggaggacgtc
     7441 gaggacctcg tggagcagcg tgtcgctcac gataggctcg cgtccgaccg ccgccggccg
     7501 cgcctcttga tccgcgcggc gcgcgccctc gcctcctcgc gcgtcgtccc ggcaggaagc
     7561 gcggcgaggt agacgtcgag gcacgcctcg cccatccgga gcgcctcggc gatccgctgc
     7621 tccgccgtcc ggccgcgagt cggccgagag gcgttcgtcg ccgcgtcccg ccggaaggcg
     7681 tcgctcacca ttcgggagaa gtctacccgc ccccgcggga cgagccgtcc cttcgaccgg
     7741 ccttgctacg atcggcgggt gactcccttc aactcggcct ccgacttcga cgtcgtcgtc
     7801 ctcggcggcg ggcccggcgg cgagcgcgcc gccatccagg ccgccaaggc gcggaaacgc
     7861 gtcgccctca tcgagaaggc ccccgtcgtg gggggcgccc gcgtgaactg ggggacgatc
     7921 ccgtcgaaga cccttcgcga gagcgccctc ttcttcctcg ggatgacgcg ccaccgcctc
     7981 cacggcttcc aggccgaggt gaccgacgac gtcacggtgg ccgacttcat gtaccgcgag
     8041 cggctcgtcg tccagcgcga gctggacatg atcaacgcct cgctcggccg ctaccggatc
     8101 gagatcttcc ggggccacgg ccgcttcctc gacccgcaca cgatcgccgt cgaggggaag
     8161 gacgggagga ggaaggggtc ggtgcggggc gaggtggtcg tcatcgcgac cggctccagc
     8221 ccgaaccgcc ccgcggacgt gccgttcgac gccgagagcg tcttcgacag caacacgatc
     8281 ctgaagctcc cgcggatgcc gaagacgatg accgtcctcg gcgccggcgt catcgggatc
     8341 gagtacgcct cgatcttcgc ggcgctcggc gtctacgtga cgctcgtcga cacgcgcgac
     8401 cggctcctcc cctacctcga ccgcgaggtc gtcggaatcc tcgagcgcga gctcggggag
     8461 ctccacgtcg agctgctcca cgacgaccgc tacgcgacgg tcgagccggt cccgggctcc
     8521 cccgcccgcg tgacgatcga gacgcggaag gggcggcgcc tcacctcgga cgtcctcctc
     8581 tactgcgtcg gccgcgacgg gaacacgaag gacctcggcc tcgaggcgat cggcctcgtc
     8641 cccgacaagt acggcctcct gagcgtcaac gccgacctcc agacgaccca cccgcacatc
     8701 tacgccgtcg gcgacgtcat cggctacccg gccctcgcct ccacctcgat ggagcagggc
     8761 cgccgcgcga tgcggcacgc cttcggcctg accggcccct cggcctcgac cgagaacctc
     8821 cccttcgcca tctactcgat ccccgaggtg agctacgtcg gcgagaccga ggagaagctg
     8881 aaggagaagg ggatcgacta cgtcgtgggg cgcggtcact acgggatgaa cccgcggggg
     8941 cagatcatcg gcgaccagga cgggctcctg aagctcctct tcgacgcgaa gagcgggcgg
     9001 ctcatcggga cgcacatcgt cgggcactcg gcgagcgagc tcgtccacgt cgggcaggcc
     9061 tacatgaaga tgcaggcgac ggcccccgag atcgccgagt cgctctacaa ctaccccacc
     9121 ctctccgaca tgtaccggca cgcggccctc accgccgtca ccgagctcgc gaagcggcgc
     9181 gggacggacg cgggctgagg aggaacggat ggcgtcgatc gagcagctcc tggggaccgt
     9241 cagcgggttc ctctggggct ggcccctcat cgtcctcctc ttcgggacgc acctcttcct
     9301 gacgctccgc ctcggcttcc cgcagcggca cctcctgaag gcgatccgga tctccctccg
     9361 gcgggagaag gagggggcgg gcgacgtcag ccagttcggg gcgctgacga ccgccctcgc
     9421 cgccacgatc gggaccggga acatcgtcgg cgtctcgacg gcgatcgccg ccggcgggcc
     9481 gggcgccgtc ctctggatgt ggctcacggg cgtcttcgga atcgcgacga agtattccga
     9541 ggcgctcctc tccgtgaagt accgcgtcac cgaccccgac gggacgatgt cgggcgggcc
     9601 gatgtacgtc ctcgagcgcg gcctgaagat gaagtggctc ggcgtcctct tcgccgcctt
     9661 caccgccgtc gccgcgttcg gcatcgggaa caccgtccag gcgaactcga tcgccgtcct
     9721 cgcgaaggag acgttcgggg tctcgccatg ggtcagcgga gggctcctga cggccctcac
     9781 cgccgtcgtc atcctgggcg gaatcaagtc gatcgcccgc gtctgcgagc tcctcgtccc
     9841 gttcatggcg ggctcctacg tcctcggctg cctctacctc ctcgcgctcc actcggagac
     9901 gctcccggac gcgttccgcc tcatcgtctc gagcgccttc accggccagg ccgcgatcgg
     9961 cggcttcctc ggcgccggga tgaaggaggc gatccgcttc ggcgtggccc gcggcctctt
    10021 ctcgaacgag tcgggcctcg ggagcgcccc gatcgtcgcc gccgccgcga tgacgaagaa
    10081 ccccgtgcgg caggcgctcg tctcctc
//
LOCUS       DAOONL010000121        10015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7717_length_10015_cov_17.937751, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000121 DAOONL010000000
VERSION     DAOONL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_10015_cov_17.937751"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /gene="pglX"
                     /locus_tag="PLP50_13965"
     CDS             <1..1224
                     /gene="pglX"
                     /locus_tag="PLP50_13965"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HPA52696.1"
                     /translation="TRASLDAARATAASLRRRQIALQEELDWQVYSLYGLLEPGVDLT
                     FASADSSDSADSSNPAASLDSVPEVALGERAFEIVLARAMAAGEVETKWFERHGSTPV
                     TEVPARWPAAYRDLVERRIQAIATNPNIALIEKPEYKRRWAQEPWEEMEARALRSWLL
                     DRLEEPRCWPVDAQGAPSLQSAVHLCDRVRHDEEFRRVAELNAGRPDVDLEKLVADLA
                     LEEAVPYLAAFRYTDDGLRKRAVWERTWDLQRREDAGETLDIPVPPKYAREDFRKGSY
                     WSLRGKLDVPKERFVHYPDASRAADPSPVLGWAGWDHLQRARALAAYYLRMKTDEGWP
                     PERLVPLLAGLAELLPWLRQWHDDLDPATGERLGTWFAAFVADEARELGTTVEALGAW
                     RPAATSARRRAKGTK"
     gene            1296..2054
                     /locus_tag="PLP50_13970"
     CDS             1296..2054
                     /locus_tag="PLP50_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52697.1"
                     /translation="MEVRPQRLPDGAPLEYAPENEQGVVYLFSTLARKNYGLRVERVQ
                     AGYPDCLATKAGRPVRIEFEYRSRNFAQHGHDPAGCDWIVCWIHDWPACPAHLRVVEL
                     RRDYGLGFNVWFQPVTGPYAEKLGRGDGFSQWSVASQASEGDLILFYRTAPDSLVRDV
                     FRVSGPVKRVEARWKAGLDWMAPIERVCTLKAPLRLEDFRANPVVKAAGFVRGAMRGR
                     PRATAFWPELHRMIVERNPALARALRRFGPERVA"
     gene            2148..2843
                     /locus_tag="PLP50_13975"
     CDS             2148..2843
                     /locus_tag="PLP50_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HPA52698.1"
                     /translation="MSDSPYPNAPVREVVFEIRFPGEPAVECRRDKLFELVRQDFPNV
                     QVPAPGPAVAGHSALLPYKFAAANGSRSLLCALNLFAFSSSLYPGFRPFRDEALRYLG
                     LFQEVVPLGRLSRTGLRYVNVIPLPPGSPVHELLDVSLSLGSYTPGELRNLALSLEIP
                     RPSGTLTMRLGPLAGSDQPMLVLDFDYAKTGDLDVAHLPEYLQESHDETKRLFEGLIT
                     QSYRAYLEGEVVA"
     gene            2840..3259
                     /locus_tag="PLP50_13980"
     CDS             2840..3259
                     /locus_tag="PLP50_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52699.1"
                     /translation="MSSVPGVMPASGVGGALLQAPSIRLADVETYRGFEDVGVAEPAR
                     LVITRAQLGGQTLRLAKPVQVELSGAPGSWVAENAALELFGDGTSRARAIQAFIERFE
                     HFLAYYLELSWDEVGGNGRRLKGLYGSIAGRDAGPNR"
     gene            3240..3548
                     /locus_tag="PLP50_13985"
     CDS             3240..3548
                     /locus_tag="PLP50_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52700.1"
                     /translation="MPVRTADIERSLPRKGFEREVSHHVYFKFRYSGKDWGISTFYSH
                     GEREVGDHLVGRMARQVKLPKADFLRLVHCPMSAEEYLELLVREGHVTAETGDSTNRA
                     "
     gene            complement(3545..4192)
                     /locus_tag="PLP50_13990"
     CDS             complement(3545..4192)
                     /locus_tag="PLP50_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52701.1"
                     /translation="MTDPASLILAVEDEPSAAVARRLVRTYRKDVVLARVIVARGYGK
                     LRVQALSYNAASVHTPFFLLTDLDRGECAPGLVENWLGGRPREASFLFRVAVREVEAW
                     LIADQAGIAKLLGIEKVLVPTDVEGLPDPKRTLVDLARHSSLRDLRRGLVPAEGSTAQ
                     VGPSYVDLVADFAERRWNPERARRAAPSLDAAIAALAGLCRERRSRPMGSRRHRA"
     gene            complement(4189..5340)
                     /locus_tag="PLP50_13995"
     CDS             complement(4189..5340)
                     /locus_tag="PLP50_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52702.1"
                     /translation="MRVTRLILKNWKNLPSVDVALGPRVFLVGPNASGKSNLLDAIRF
                     LRDVVVEGGLQKALGESRGGLSKIRSLSARKDPEVRIEIHLGGPSRSAPSWRYALALR
                     QETTGARATRITEERAWKGEALVLDRPGAADRADSRRLEQTSLESLSANAEFRPVADF
                     LREVTYLHLVPQLLRFPQAFTGPRMPSDPYGRSFLERVASATPKTRNARLRRIESALK
                     AAVPNLEALRYVEERGVPHLEAVHKHWRPKAGKQREEQFSDGTLRLIGLLWSLLEGES
                     LLLLEEPELSLNPAIVRRLASMIHGMQRTEGRQVVISTHSPDLLSDPGIGGEEILLLT
                     PRAEGTEVRLASDRREVRALLESGMTVADAVLPLAEPAETRQLALFAEP"
     gene            5447..6628
                     /locus_tag="PLP50_14000"
     CDS             5447..6628
                     /locus_tag="PLP50_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52703.1"
                     /translation="MQHLPQPEEQRWILDGLASLIRRGGLAPFVSAPIVETNRRYFPE
                     GYSPTLPSVDRLARRLMQYAGLGDLDVRLEPFEPKEVDDESVLADFRGIEKGVATFGL
                     NVEQVADLEPIAGVLAHEVAHAFRQRRGLVVDDPDEEELLTDLTTIYLGFGILTANAR
                     WRFRKGGELQGAYAVTRWVTTAAGYLPAQGFSFALAAQLVARDPAPREWKRVLAALET
                     NQRAFAETALEELAADRERLATTLALPDRTDWPPPVAPDSILRPLPPPPEFEEWGDTE
                     EDTTWNAGHPVFRVRESHVVLSTVLPSLIFGIGGMALTGVLWGSQLGFWAGAAGAAIG
                     LRTGLKGRCDVCSDPACKAKIPEPAATCPGCGGTVAGRIDHPDQRLEAEERLGQPRMM
                     R"
     gene            6625..7542
                     /locus_tag="PLP50_14005"
     CDS             6625..7542
                     /locus_tag="PLP50_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPA52704.1"
                     /translation="MTRHERSSHTAPSAETVAAARLFDDGGPLLFGEETGDGFPWTGI
                     VVHSSSCDSPTCNCRDVDFLARPLVPDGAAFVEHKEVSVRGQLDLDTGRITPAAEPGA
                     ATPQAALLARVRLRLAGDRLELLRARWHRAKHQDDPDEWRTVDWGRVDLTANVPFCEV
                     LPSRWDLAVGFERHHWWIFDLWCLKPGCTCETVGLSFHRDDGSAQELVEVDLPRRSLT
                     GRGTSETGRGLWEALRDDEELLAELESRRATIRRVARQLPAFLTPAKPAAAPAPVGRN
                     EPCPCGSGKKFKRCCGGVAAGPRERGTTR"
     gene            7539..8759
                     /locus_tag="PLP50_14010"
     CDS             7539..8759
                     /locus_tag="PLP50_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52705.1"
                     /translation="MRTTTTRHPWQFKARFRRHAFGWRSQPAITRVKEAVAEIRKVAR
                     KDGLLAAEGAVTFLERVSPALEQVDSSSGAIGTAVRHAIESLVPIIASAPADAKTREA
                     WLERLNEAHGNDEIPYIERLADHWGDLCASKEVASAWADRLLWITKRILGPDKEPGAF
                     FHGTSACLSALFRAERYEELLELLRDEKFWHYRRWAVKALAATGRDDDAISLAESLRG
                     PWTSDLEVDSLCEEILLSSGRADEAYERYALRANRAGTYLAAFRAVVRKYPEKTAAEV
                     LAGLVASTPGDGGKWFAAAKEAGLYDEALALARRAPCDPKTLTRAARDYAPTEPEFAL
                     NSGLLALHWLVAGYGYEITGADVQEAYRSTMAVAERHGMGTEVKERVRTLVASEKTSD
                     RFVSKVLGRELQLP"
     gene            8815..9036
                     /locus_tag="PLP50_14015"
     CDS             8815..9036
                     /locus_tag="PLP50_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52706.1"
                     /translation="MNGRGEAERQAAAALVEDVGRLRKDDAVFHDVLGFRGSRVPVTA
                     VVADLSDPKDPGLRKAIARVKRAAVRTSS"
     gene            9068..9328
                     /locus_tag="PLP50_14020"
     CDS             9068..9328
                     /locus_tag="PLP50_14020"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPA52707.1"
                     /translation="MVREAAMPSLTIRDIPEDLHEKLKADAAAQGRSLNKEILIRLRL
                     STEPRKPPVDELLRRARENRERMPGVWITNEVINRAKRRGRA"
     gene            9325..9735
                     /locus_tag="PLP50_14025"
     CDS             9325..9735
                     /locus_tag="PLP50_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA52708.1"
                     /translation="MIVVDTNVIAYLWVDGPMTASAVALARADDEWVAPPLWRSEMRN
                     LLATLARAGRLTELQALRSAANAESQMRGAEQVVETSDVLTLAVRSGCSAYDCEFVAL
                     AVSLGVPFVTCDRKLVRAFPDVARLLPGRVEAES"
     gene            9732..>10015
                     /locus_tag="PLP50_14030"
     CDS             9732..>10015
                     /locus_tag="PLP50_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HPA52709.1"
                     /translation="MTLLKDLIRVPERVHQGDFVLKLTDGVERPKETLGSYVVTPQLR
                     EAFDAALSLVGSAVKEKTSKAALLHGSFGSGKSHFMAVLYQLLKHDADARS"
BASE COUNT         1498 a         3573 c         3474 g         1470 t
ORIGIN      
        1 acccgcgcct ccctcgacgc cgcccgcgcc accgccgcct ccctccgccg tcgacagatc
       61 gctctccagg aagagctcga ctggcaggtc tactcgctct acggcctcct cgaacccggc
      121 gtcgacctga ccttcgcctc cgccgacagc tccgacagcg ccgacagctc caacccggcc
      181 gcctccctcg actccgtccc cgaggtcgcc ctcggcgagc gcgccttcga gatcgtcctc
      241 gcgcgggcga tggccgcggg cgaggtcgag acgaagtggt tcgagcggca cggctcgaca
      301 ccggtcaccg aggtccccgc gcgctggccg gcggcctatc gcgacctcgt cgagcgccgg
      361 attcaggcca tcgccacgaa cccgaacatc gccctcatcg agaagccgga gtacaagcgg
      421 cgctgggcgc aggagccgtg ggaagagatg gaggcccgcg ctcttcgctc ctggctcctc
      481 gaccgcctcg aggagccgcg ctgctggccc gtcgacgcgc agggggcgcc ctcgcttcag
      541 tcggcggtcc acctctgcga ccgcgtccgc cacgacgagg agttccgccg cgtcgccgag
      601 ctcaacgccg gccgacccga cgtcgacctc gagaagctcg tcgccgacct cgccctcgag
      661 gaggccgtcc cctacctcgc cgccttccgc tacaccgacg acggcctgcg caagcgcgcc
      721 gtctgggagc ggacgtggga cctccagcgc cgcgaggacg cgggcgagac gctcgacatc
      781 cccgtcccgc cgaagtacgc ccgcgaggac ttccgcaagg gctcgtactg gtcgctccgc
      841 ggcaagctcg acgtcccgaa ggagcgtttc gtccactacc ccgacgcctc ccgcgccgcc
      901 gacccctccc ccgtcctcgg ctgggccggc tgggaccacc tccagcgcgc ccgcgccctc
      961 gcggcctact acctccggat gaagaccgac gagggctggc cccccgagcg cctcgtcccc
     1021 cttctcgccg gcctcgccga gctcctcccc tggctcaggc agtggcacga cgacctcgac
     1081 cccgccaccg gcgaacgcct cggcacctgg ttcgccgcct tcgtcgccga cgaggcgagg
     1141 gagctgggga cgacggtgga ggcgctcggg gcgtggcggc cggcggcgac gagcgcgcga
     1201 cgccgcgcga agggaacgaa gtgagcgcct ccacaaaggc ccggcggcga cggacgagta
     1261 ctccccgaaa gggacgcccc gcggcgccgg ccgacatgga agtccgcccg caacgcctcc
     1321 ccgacggtgc gccgctcgaa tacgcgcccg agaacgagca gggcgtcgtc tacctcttct
     1381 cgacgctcgc gcgaaagaac tacggcctgc gggtggagcg ggtccaggcg ggataccccg
     1441 actgcctcgc cacgaaggcc ggccggccgg tgaggatcga gttcgagtac cggtcacgga
     1501 acttcgcgca gcacggccac gaccctgcgg gatgcgactg gatcgtctgc tggatccacg
     1561 actggccggc ctgccctgcc cacctccgcg tcgtcgagct ccggcgcgac tacggcctcg
     1621 gcttcaacgt ctggttccag cccgtcaccg ggccgtatgc cgagaagctg ggtcgaggcg
     1681 acgggttcag ccagtggtcg gtcgcgtcgc aggcgtccga gggcgacctc atcctcttct
     1741 accggaccgc tcccgacagc ttagtccgcg acgtcttccg ggtcagcgga cccgtgaagc
     1801 gggtcgaggc tcggtggaag gccggcctcg actggatggc gccgatcgag agggtctgca
     1861 ccctgaaggc gcctctacgc ctggaagact tccgcgcgaa ccccgtcgtg aaggccgccg
     1921 gcttcgtccg cggagcgatg cgcggccgcc ccagggcgac cgcgttctgg cccgagcttc
     1981 accggatgat cgtggagagg aacccggccc tcgcgagggc gcttcgccgg ttcggcccgg
     2041 agcgggtcgc ctgaggcttc ggcccgaccg cgcgcggtgg cgggaggcag ggccatcggc
     2101 tacagttcgc gcatgaaccg tcggcgggag aagagactgg caagtcgatg agcgattcgc
     2161 cctatcccaa cgcacccgtc cgggaggtcg tcttcgagat ccggttcccc ggtgagccgg
     2221 cggtggagtg ccgtcgggac aagctcttcg agctggtgcg tcaggacttc ccgaatgtgc
     2281 aagtaccggc cccgggtccg gccgtggcgg gccattcggc cctcctcccc tacaagttcg
     2341 ccgcggcgaa cggctcgcgt tccctcctct gtgccctcaa tcttttcgcc ttttcctcgt
     2401 ctctctatcc cggcttccgt ccgttccggg acgaagcgct tcgatatctg ggactcttcc
     2461 aggaggtcgt accgctcggt cggctatccc ggaccggcct ccgctacgtg aacgtcattc
     2521 ccctgccccc cggctcgccc gtccacgagc tgctcgacgt cagcctctcg ctcggcagct
     2581 acacccccgg cgagctccgg aacctcgccc tcagcctcga gatcccgcgc ccttcgggga
     2641 cgctcacgat gcggctcggg ccgctcgccg gctccgacca gccgatgctc gtgctcgact
     2701 tcgactacgc caagaccggc gacctcgatg tcgcgcacct tcccgagtat ctgcaggaga
     2761 gccacgacga gacgaagagg ctcttcgaag ggctcatcac ccagagctat cgggcctacc
     2821 tcgagggaga ggtcgtcgcg tgagctccgt ccctggcgtg atgcccgcct ccggcgtcgg
     2881 aggggccctc cttcaggcgc cgtcgatccg gctggcggac gtggagacgt acaggggctt
     2941 cgaagacgtc ggcgtcgcgg agccggcccg gctcgtcatc acgagggcgc aactcggcgg
     3001 gcaaaccctc cggctcgcga agccggtcca ggtcgagctg agcggcgcac ccggctcctg
     3061 ggtcgcggag aacgccgccc tcgagctctt cggtgatggt acttcccggg cgagagcgat
     3121 ccaggccttc atcgagcgtt tcgagcattt cctcgcgtac tacctcgagc tctcttggga
     3181 cgaggtcggc gggaacggac gacgcctgaa gggcctctac gggtccatcg cgggtcggga
     3241 tgccggtccg aaccgctgac atcgaacgct cgctcccccg aaagggcttc gagcgggaag
     3301 tctcccatca tgtctatttc aagtttcgct acagcggcaa ggactgggga atctccacct
     3361 tctactctca tggtgagcgg gaggtcggcg atcacctcgt cggccgcatg gcgaggcagg
     3421 tcaagctccc gaaggcggac ttcctccggc tcgtccattg cccgatgagt gccgaggagt
     3481 acctcgagct tctcgtccgc gaaggacacg tcactgccga aacgggggat tctacgaacc
     3541 gggcttaggc tcggtggcgg cgactcccca tcggcctcga cctcctttcg cggcagagtc
     3601 ctgccagggc cgcgatggcc gcgtcgaggc tcggcgcggc cctgcgcgcc cgctccgggt
     3661 tccatcgtct ttccgcgaag tcggcgacca ggtcgacata cgacgggcca acctgcgccg
     3721 tgctcccctc ggcggggacg aggccccggc gcaggtcgcg gaggctcgaa tggcgcgcga
     3781 gatcgacgag cgtgcgcttc gggtccggga gcccttcgac gtccgtcggc acgaggacct
     3841 tctcgatgcc gaggagcttc gcgattccgg cctggtcggc gatgagccag gcctcgacct
     3901 cccgcacggc gacgcggaag aggaacgacg cctcgcgggg cctgccgccc agccagtttt
     3961 cgacgaggcc gggagcgcac tcgcccctgt cgaggtcggt aagcaggaag aacggcgtgt
     4021 ggacggaagc cgcgttgtag cttaatgcct gcactctgag cttcccgtac ccccgggcga
     4081 cgatgacgcg ggcgaggacg acgtccttgc gatacgttcg gacgagccgg cgcgcgacag
     4141 cggcgctcgg ctcgtcctcc accgcgagga tcagcgaggc gggatcggtc acggctcggc
     4201 gaagagagcc agctgacggg tctccgcggg ctccgcgagg ggcaggacgg cgtcggcgac
     4261 ggtcatgccc gactcgagga gcgcgcggac ctcgcgcctg tcggaggcga gccgcacttc
     4321 ggtcccctcc gcccggggcg tcagaaggag gatctcctct ccgccgatgc cgggatcgga
     4381 gagcaggtcc gggctgtggg tcgagatcac gacctgccgc ccctcggtcc gctgcatccc
     4441 gtggatcatc gacgcgagcc gccggacgat ggcgggattc agggagagct cgggctcctc
     4501 gaggagaagg agagactccc cctccagcag cgaccagagg agaccgatga ggcgcagcgt
     4561 gccgtctgag aactgctcct cgcgctgctt ccccgccttc gggcgccagt gcttgtgaac
     4621 cgcctcgaga tgcggcacgc cccgttcctc gacgtacctg agcgcctcga ggttcggcac
     4681 cgcggccttc agagccgact cgatgcgccg gagccgggcg tttcgcgtct tgggcgtcgc
     4741 ggacgcgacc cgctcgagga agctccggcc gtaggggtcg ctcggcatcc ggggcccggt
     4801 gaacgcctgc gggaatcgta ggagctgcgg cacgaggtgg aggtacgtca cctccctgag
     4861 gaagtcggcc acggggcgga actctgcgtt ggcgctcagg gattcgaggc tcgtctgctc
     4921 gaggcggcgg gaatccgccc ggtcggccgc cccggggcgg tcgaggacga gggcctcacc
     4981 cttccaggca cgctcctcgg tgatgcgcgt cgcgcgcgca ccagtcgtct cctgccgaag
     5041 cgcgagagcg taccgccacg agggagcgga gcgggacgga ccgccgaggt ggatctcgat
     5101 ccgcacctcc gggtccttgc gggcggaaag cgaccggatc ttcgacagcc cgccacggga
     5161 ctcgccgagc gccttctgaa gtccgccttc aacgacgacg tcacggagga accggatggc
     5221 gtcgaggagg ttcgacttcc ccgaggcgtt cggcccgacg aggaagaccc gcggccccag
     5281 ggcgacgtcg acgctcggga ggttcttcca gttcttcagg atgagccgcg tcacgcgcac
     5341 cgggtccgtt ccgtgggttc tcactcatcc cagtgtatcg ccgggccgga cgagcatgag
     5401 aggggactgg agaggggcca atccgtcggc tagagttccc gcctgcatgc agcacctccc
     5461 ccaacccgaa gagcagcgct ggatcctcga cggcctcgcg agcctgatcc gcaggggcgg
     5521 cctcgccccg ttcgtttcgg cgccgatcgt cgagacgaac cgacgctact tccccgaggg
     5581 ctactcgccg acgctcccgt cggtcgaccg gcttgcccgc cgcctgatgc agtacgccgg
     5641 gctcggggac ctcgacgtga ggctcgagcc gttcgagccc aaggaagtcg acgacgagag
     5701 cgtcctcgcg gacttccgcg gtatcgagaa gggggtcgcg acgttcggcc tgaacgtcga
     5761 gcaggtggcc gacctcgagc cgatcgcggg ggtgctggcc cacgaggtcg cccacgcgtt
     5821 ccggcagcgc cgcggcctcg tggtcgacga tccggacgag gaggagctgc tcaccgacct
     5881 gacgacgatc tacctcggct tcggcatcct gacggcgaac gcgcggtggc gtttccgcaa
     5941 aggcggcgag cttcaggggg cctacgccgt gacgaggtgg gtgacgacgg cggccggcta
     6001 ccttccggcg cagggctttt cgttcgccct tgccgctcag ctcgtcgccc gcgaccccgc
     6061 gccgcgcgaa tggaagagag tcctggcggc gctcgagacg aaccagcggg cgttcgccga
     6121 gacggccctc gaggagctcg cggcggatcg cgaacggctc gcgacgaccc tcgccctccc
     6181 cgatcgcacc gactggccgc cgccggtggc gcccgattcg atcctccggc cgcttcctcc
     6241 tccgccggag ttcgaggagt ggggagacac cgaggaggac acgacctgga acgcggggca
     6301 cccggtcttc cgggtgcggg agagccacgt cgtcctctcg accgtcctgc ccagcctcat
     6361 cttcggcatc ggggggatgg cgctcaccgg tgtcctctgg ggctcgcagt tgggcttctg
     6421 ggcgggtgcg gccggtgccg cgatcggcct tcggacgggg ctgaaagggc gctgcgacgt
     6481 ctgctccgac ccggcctgca aggcgaagat ccccgagccc gcggcgacct gccccggctg
     6541 cggcgggaca gtcgccggcc ggatcgacca tcccgatcag aggctcgagg cggaggagcg
     6601 gctggggcag ccgaggatga tgcgatgacc cgtcacgaac gctcctcgca cacggctcct
     6661 tcggccgaga cggtggcggc ggcgcggctc ttcgacgacg gagggccgtt gctcttcggg
     6721 gaggagaccg gcgacggctt cccatggact ggaatcgtcg tccacagctc gagctgcgac
     6781 agcccgacct gcaactgcag ggacgtcgat ttcctcgcgc gcccgctcgt ccccgacggc
     6841 gcggctttcg tcgagcacaa ggaggtctct gtccgcgggc agcttgatct cgacacgggg
     6901 aggatcacac cagctgccga gcccggggcc gcgactccgc aggcggcgct ccttgcacgc
     6961 gtccgtctcc gcctcgcggg ggatcggctg gagctgctcc gggcccgctg gcatcgcgcg
     7021 aagcaccagg acgaccccga cgagtggagg acggtcgact ggggccgggt cgatctcacc
     7081 gcgaacgtcc cattctgcga ggtccttccg agccgctggg atctcgccgt cggcttcgaa
     7141 cgccatcact ggtggatctt cgacctctgg tgcctgaagc ccggctgcac gtgcgagacg
     7201 gtcggcctca gcttccaccg cgacgacggg agcgcgcagg agctcgtcga ggtcgacctc
     7261 ccgagacgtt cgctcacggg aagggggacg agcgagacgg gccgcggtct ctgggaggcg
     7321 ctccgagacg acgaggagct cctcgccgag ctcgagtctc ggcgcgcgac gatccggcgc
     7381 gtggcccggc agctccccgc gtttctgacc ccggcgaagc ctgcggccgc gcccgcgccg
     7441 gtcggccgga acgagccgtg cccctgtggg tcggggaaga agttcaagcg ctgctgcggc
     7501 ggggtggcag ccgggccccg ggaacgcggg accacccggt gagaacgacg acgacgcgcc
     7561 acccatggca gttcaaggcg cgcttccgcc ggcacgcctt cggttggcgg tcgcagccgg
     7621 cgatcacgcg cgtgaaggag gcggtcgccg agatccggaa ggtcgcccgg aaggatgggc
     7681 tgctcgccgc cgagggcgcg gtcacgttcc tcgaacgcgt ctcccccgcc ctcgaacagg
     7741 tcgacagctc gtcgggggcg atcgggaccg ccgtgcgcca cgccatcgag tccctcgtgc
     7801 ccatcatcgc cagcgccccc gccgacgcga aaacgcgcga ggcgtggctc gagcgcctga
     7861 atgaggccca cgggaacgac gagatcccgt acatcgagcg cctcgcggat cactggggtg
     7921 acctctgcgc gtcgaaggag gtcgcctccg cctgggcgga ccggcttctc tggatcacga
     7981 agcggatcct cgggccggac aaggagccgg gcgccttctt ccacggcacc tcggcctgcc
     8041 tttccgccct cttccgcgcc gagcgctacg aggagctcct cgagctcctg cgcgacgaga
     8101 agttctggca ttaccggcgc tgggccgtga aggcgctcgc ggcgacgggg cgagacgacg
     8161 acgccatttc cctcgcggaa tcgctccgtg ggccctggac gagcgacctc gaggtcgact
     8221 cgctctgcga ggaaatcctc ctgtcgtccg gacgggccga cgaggcgtac gagcgctacg
     8281 ccctgcgcgc gaaccgggcc ggcacttatc tcgcggcgtt tcgggccgtg gtccggaagt
     8341 accccgagaa gaccgcggcg gaagtcctgg ccggcctcgt cgcgtcgacg cccggggacg
     8401 gagggaagtg gttcgcggcg gcgaaggaag ccggcctcta cgacgaggcc ctcgccctgg
     8461 cccggcgcgc tccctgcgat ccgaagaccc tgacgcgcgc ggcccgcgac tacgccccga
     8521 cggagcccga attcgcgctc aactcgggac tcctcgcgct ccattggctc gtggcgggct
     8581 acggctacga gatcaccggc gccgacgtcc aggaggccta tcggtcgacg atggccgtgg
     8641 ccgaacggca cggcatgggg accgaggtga aggaacgggt ccggacgctg gtcgcctcgg
     8701 agaagacgag cgaccgcttc gtgtcgaagg tcctcggaag ggagctgcaa ctgccatgag
     8761 ggaacgggcg ctgatccacg tcgcgggtcc cgccggcgcg ggaaagacgt cgtggtgaac
     8821 ggacgcggcg aggcggagcg gcaggccgcg gccgcgctcg tcgaggacgt cgggcggctt
     8881 cgaaaggacg acgcggtctt ccacgacgtg ctgggctttc gcggaagcag ggtccccgtc
     8941 accgccgtcg tcgcggacct gtcggacccg aaggaccccg ggctgaggaa agcgatcgcc
     9001 cgcgtgaagc gggcggcggt gcgaacgtcc tcgtaaacgc gtgcagtgac atcattccgg
     9061 ttctagaatg gtgcgggagg ccgcaatgcc cagcctcacg atccgcgaca ttcccgagga
     9121 cctgcacgag aagctcaagg ccgacgccgc cgcccagggt cgaagcctga acaaggagat
     9181 cctgatccgg ctccggctct ccacggagcc gcggaagcct cctgtggacg agcttctgcg
     9241 gcgagcccgg gagaaccgtg agcgtatgcc cggggtctgg atcacgaacg aggtcatcaa
     9301 tcgggccaag aggcggggcc gagcgtgatc gtcgtcgaca cgaacgtcat tgcctacctc
     9361 tgggtcgacg gccccatgac cgcgagcgcc gtggcgctcg cccgggccga cgacgaatgg
     9421 gtcgccccgc ccctctggag gtccgagatg cggaacctcc tcgcgacgct tgcgagggcc
     9481 ggccggttga cagagctcca ggcccttcgg tcggccgcga acgccgaaag ccagatgcgg
     9541 ggcgccgagc aggtggtcga gacgtccgat gtcctcaccc tcgcggtgcg ctccggctgc
     9601 tccgcctacg actgcgaatt cgtcgccctc gccgtctcgc tcggcgtccc tttcgtcacg
     9661 tgcgaccgaa agctcgtgag agcgttcccg gatgttgcgc gccttcttcc cgggagagtg
     9721 gaggccgaat cgtgaccctc ctcaaggacc tcatccgcgt ccccgagcgc gtccaccagg
     9781 gcgatttcgt cctcaagctg acggacgggg tcgagcggcc gaaggagacg ctcgggagct
     9841 acgtcgtcac gccgcagctc cgcgaggcgt tcgacgcggc cctctcgctc gtcgggagcg
     9901 ccgtgaagga gaagacgagc aaggcggccc tcctccacgg gagcttcggg agcggcaaga
     9961 gccacttcat ggcggtgctc taccagctcc tgaagcacga cgccgacgcg cggtc
//
LOCUS       DAOONL010000122         9948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7809_length_9948_cov_11.743253, whole genome shotgun sequence.
ACCESSION   DAOONL010000122 DAOONL010000000
VERSION     DAOONL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_9948_cov_11.743253"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLP50_14035"
     CDS             complement(<1..287)
                     /locus_tag="PLP50_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPA52710.1"
                     /translation="MACGLLLAAATACARGGERGEAVRLWAFGREGEVVRELVPELER
                     RHPGLRVEVQQIPWTAAHEKLLTAYVGRELPDVAQMGNTWIPEMAALDAIEP"
     gene            complement(293..1816)
                     /locus_tag="PLP50_14040"
     CDS             complement(293..1816)
                     /locus_tag="PLP50_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPA52711.1"
                     /translation="MIPRPASRAVATLLLAALAAGCGSPSSARAGEEPASPARVVPSP
                     DAGELPVPSRVLDPLVEELQHRTFRWFWETANPRNGLVPDRWPAPSFSSVAAVGFGLS
                     AYLVGAERGWVTREEARERVLVTLRFLWNAPQGTAAEGMTGYRGFFYHFLDMETGKRW
                     GKDVELSSIDTTLCLAGALSAGLYFDRDDAAEAEVRDLAKRLYERVDWRWMQPRPPRV
                     AMAWLPETGFGPADWHGYDESMVLYLLALGSPTHPATDGAWETFVSTYRWAEHEGQTY
                     VNFAPLFGHQFSHIWVDFRGIRDAFLREKGIDYFENSRRAVLAQRAYAIRNPEGFLGY
                     GPDVWGLSACDGPADATLTVDGRARKFVTYGARGVSSFWTHDDGTITPMAAAASLPFA
                     PEVVLPAIREMKKLHGEHLWGEYGFFDAFNPTFRFNDVPTPLGKVVPEAGWYATNYLG
                     IDQGPIVLMLENQRSGLLWRLMKRSPPIREGLRRAGFTGGWLEEGAAPARSGEAATR"
     gene            complement(1827..4691)
                     /locus_tag="PLP50_14045"
     CDS             complement(1827..4691)
                     /locus_tag="PLP50_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA52712.1"
                     /translation="MRSSPGPKALFVLPAVLLAVATLVAPPASGQTTTAQVRGTVTDA
                     QGPIPSMPVIAVNTATGARFSAATATNGAFVLVLPAAAYELSVSTGAHAPWKRSIQVG
                     VGQSLQLDVALQPASITAEVAVVATAAEAQVERVTSEVATNVSEIQIQSLPQSSRNFL
                     NFAALAPGVRLSREELRQEYSYGAQGSSNTNVFIDGTSYKNDVLLGGSVGQDASRGNP
                     FPQSAVQEFRVITQNFKAEYQKASSAIITAVTKSGGNELHGDVFVYYQNKDLVAIDGV
                     TQRRADANNTEAVKPEYERWQPGISLGGPIVKDRLHFFASYEGNVQDREYEVFRGTNM
                     NWPAAFRDQFARYEGLYTSPFRSTLLFGKVTASLAENSHLDVSGDLRHETDIRGFGAQ
                     TSYQAAENVKNDVWTVRGKHTAVLGSFLNEATASFQRFKWNPVPQDDSLPGMNYDGLM
                     RVGSKDTQQNFTQDRFALRDDVTLMNLTWNGDHVVKGGVTYDYLKYHVVKQLNGNPVF
                     NFRNSTYVTTPGDMPYNAIYGVGDPDLSTNNTQFGLYVQDDWRITSRFTANVGLRWDF
                     ETDMLNNDYETPADIRAAFAGIYPSNYLTDGTQRDTFYGAIQPRVGLSWDVRGDGKTI
                     LHAGYGKYYDRTLYNDILDEKYRLQYKVTTFWFSPDGGPMDGRPAIKWDPRYLSLTGL
                     QELLASGEAPQPELFFLANDTRPPSSDQWSLGARHDFGFFNAAVTYTGVKSKDQLTWT
                     CGIKNADGTCNWGARPAPGYGFSMLSRSKEAWFDSVQVVLDKPFSATAGWGAFLSYVW
                     SDAEQTGNDLFSWGILDPVNGIRQRSAGSQEHQITLSGIVGLPWEIRASTLVTLGSGY
                     PFGGTDCSAGWDKCVDHVGAGDPPKWTQSIDVRLEKRFALGGSYDVNVFAEAINVFNY
                     TNEKGYDGWQPALPEINANYGKPNEGYNPRRIQLGASFGF"
     gene            complement(4688..5848)
                     /locus_tag="PLP50_14050"
     CDS             complement(4688..5848)
                     /locus_tag="PLP50_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA52713.1"
                     /translation="MARDERRARGTRSAGRTSGEAGAPTGGIREVARAAGVSVATVSR
                     VVNGTAVVRDETKRRVETEIARLRYAPHAAARSLITNRTSTVGVVLPDIWGEYFSEVI
                     RGIDLAARQAGYHVLVSSSHSDAAETRDVLRAMNGRVDGVVVMAPHASPRALSSAMPA
                     GLPSVFLNSAPGGADAPSLSVDNRGGARAMVAHLLALGHRRLALLNGPGSNRDAAERR
                     RGAFDAARAAGLAAESVLEIQGDFAEESGRVAGEELLRRSPRPTALFAANDSMAIGAL
                     YALRRARAAIPADVAVAGFDDVPIARFASPPLSTVRHDIRSLGERALARLVAEIDGRG
                     DGLPAREVLPFHLVLRYSTEGIDTETTTEPSRPPRAGAPTPEPTTDRRRKAR"
     gene            5957..7483
                     /locus_tag="PLP50_14055"
     CDS             5957..7483
                     /locus_tag="PLP50_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA52714.1"
                     /translation="MNHRSLRAVLVVLGMAVASCRDVRVRPLPDPVRLARQTSVLSLD
                     PLETEPRTASALANVFEPLVAYDRNLELVPALAIRWETPDEKTWDFTLRPGVSFHDGT
                     PLDAEAVVAALDRARLSPESGVRGTLWAVSRVEATDDRTVRVITRVPDALLLHELTLV
                     LVARGATRARVEAQPVGTGPYRVVSWRKGEALELEAWDGYWGAAPSLRRLRVVPLPPD
                     VDGIEAVARGEAEVAELPATSARRTSRGDVRIVSSPGLTTFYLWMNGPRMIGGRPNPF
                     ADARVRRAAALAVDRARLARAATGTDATAAPQLVPPTVYGHVPAMTAPAHDPEAARAL
                     LRSTGFSLPVEATLAYRAVGTEDPAARLLQEMLETAGFRVTLRPVPWDQIQAELRDGR
                     LSLFFGGWVFDGPDAGGFLRDCFRSRGPDGLTGFFNPGYSHPEIDRLVDASYGAFFRT
                     SRLLILEETMRIANEDAPLVPLFHRPDAWAVAPGLLWEPRPDGKVLAAEMRPAGAPGS
                     "
     gene            7568..9442
                     /gene="sppA"
                     /locus_tag="PLP50_14060"
     CDS             7568..9442
                     /gene="sppA"
                     /locus_tag="PLP50_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPA52715.1"
                     /translation="MNSERPRSLPRRVLSAIGRGLAFVRNTFLNVLALSFVVGLLAAL
                     FAGGPKVEKGSALVLVPKGNIVEQLSAKSPSDLVENLAGGGTEETLLKDVIDALAAAK
                     TDERISSVYLDLDEMGGAGMTVLEDVAAALADFRSSGKKVVVAGDLFDMRRYFLAAQG
                     DELLLHPQGMVLIEGFGRYRSYYKDAIDRLGVTWNVFRVGEYKSAVEPFLRNDMSPEA
                     KEADLEWMGDLWRAYLASVAKARKTTPEELTALIEQLPERLKASGGDPAKLALDAKLV
                     DRLATRDEVRTRMIELAGEDAKSKSFRQVAMGDYLEALGSDRPGAKGKGDAVAVVVAK
                     GEIVDGTAGPGRVGGDSTAKLIRQARQDEKVRAIVLRVDSPGGSAFASEVIRRELQLA
                     REAKKPVVVSMGSVAASGGYWISTSSDEIWASPETITGSIGIFGMFPTIEKPLAKYLG
                     IRVDGVGTTPWAGLRIDRELPPRAGEAIQAMIDQGYEEFLERVGKARKMTRDQVDKIA
                     RGRVWSGADAKGIGLVDHLGGLGKAIESAAAKAKLGKSYRVTYVEKEKSLKEKLLSSL
                     SVRATRLAAAFGYALAPAAETADGPGAALLGAVAAEVGQLSVWNDPRGVYAHCLCEVK
                     "
     gene            complement(9538..>9948)
                     /locus_tag="PLP50_14065"
     CDS             complement(9538..>9948)
                     /locus_tag="PLP50_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52716.1"
                     /translation="MNALLSLSEKPPIGQRLADAVVVAGLEAAKGNGRRAVVLILGPG
                     AKDEGLLGAEEAMSFLSRLVVPFQVWSAGRKHSREAVRWNAQRLMSDTREVEGAVHDL
                     LETTIRQRIVWVEGSHLPQDVELGPLAKGIRLAR"
BASE COUNT         1378 a         3556 c         3523 g         1491 t
ORIGIN      
        1 ggctcgatcg cgtcgagcgc ggccatctcc gggatccagg tgttccccat ctgcgccacg
       61 tcgggaagct cccggccgac gtaggccgtc aggagcttct cgtgcgccgc cgtccagggg
      121 atctgctgca cctcgacgcg gaggccgggg tgccgcctct cgagctcggg gacgagctcg
      181 cggacgacct cgccctcgcg gccaaaggcc cagaggcgga ccgcctcgcc gcgctctccc
      241 ccgcgcgcgc aggccgtcgc cgcggcgagg aggaggccgc aggcgatcgc cctcaacggg
      301 tcgccgcctc cccgcttcgc gccggcgccg ccccctcctc gagccagccg cccgtgaacc
      361 cggcgcgacg gagcccctcg cggatcggcg gcgaccgctt catgagccgc cagaggagcc
      421 cgctcctctg gttctcgagc atcaggacga tcggcccctg gtcgatcccg aggtagttcg
      481 tcgcgtacca cccggcctcc ggcaccactt taccgagcgg cgtcgggacg tcgttgaacc
      541 ggaacgtcgg gttgaacgcg tcgaagaagc cgtattcgcc ccagaggtgc tcgccgtgga
      601 gcttcttcat ctcccggatc gcggggagga cgacctccgg cgcgaacggg agcgaggcgg
      661 cggcggccat cggggtgatc gtcccgtcgt cgtgcgtcca gaacgaggag acgccgcggg
      721 cgccgtaggt gacgaacttc cgggccctcc cgtcgaccgt gagcgtcgcg tcggccgggc
      781 cgtcgcaggc cgagaggccc cagacgtcgg gcccgtagcc gaggaagcct tccgggttcc
      841 ggatcgcgta ggcacgctgg gcgaggacgg cccggcggga gttctcgaag tagtcgatcc
      901 ccttctcccg caggaaggcg tcccggatcc cgcggaagtc gacccagatg tgcgagaact
      961 ggtggccgaa gagcggcgcg aagttcacgt aggtctgccc ctcgtgctct gcccacctgt
     1021 aggtcgagac gaacgtctcc cacgccccgt ccgtcgccgg gtgcgtcggc gagccgagcg
     1081 cgaggaggta gagaaccatc gactcgtcgt agccgtgcca gtccgccggt ccgaagccgg
     1141 tctccggcag ccaggccatg gcgacgcggg gcgggcgcgg ctgcatccac cgccagtcga
     1201 cccgctcgta gagcctcttc gcgaggtcgc ggacctcggc ctccgccgcg tcgtcgcggt
     1261 cgaagtagag ccccgccgag agggccccgg cgaggcagag cgtcgtgtcg atcgaggaga
     1321 gctcgacgtc cttcccccac ctcttccccg tctccatgtc gaggaagtgg tagaagaacc
     1381 ccctgtagcc cgtcatcccc tcagccgccg ttccctgcgg ggcgttccag aggaaccgga
     1441 gggtgacgag gacgcgctcc cgcgcctcct cgcgggtcac ccagccccgc tcggccccga
     1501 cgaggtacgc cgagaggccg aagccgacgg cggcgacgct cgaaaacgac ggggcgggcc
     1561 agcggtcggg gacgaggccg ttccgcgggt tcgccgtctc ccagaaccag cggaacgtcc
     1621 ggtgctgcag ctcttcgacg agagggtcga ggacccggga agggaccggg agctcgcccg
     1681 cgtccgggga cgggacgacg cgcgcgggcg aggccggctc ctcacccgcg cgcgcggagg
     1741 acggggagcc gcagcccgcg gcgagcgccg caagcaggag cgtcgcgacg gctcgggacg
     1801 cgggccgcgg gatcaagagc gcctcttcag aagccgaagc tcgcgccgag ctggatccgg
     1861 cgcgggttgt agccctcgtt gggcttgccg tagttcgcgt tgatctcggg gagcgccggc
     1921 tgccagccgt cgtacccctt ctcgttcgtg tagttgaaga cgttgatcgc ttcggcgaag
     1981 acgttcacgt cgtacgagcc gcccagggcg aagcgcttct cgagccggac gtcgatgctc
     2041 tgcgtccact tcggcgggtc gccggcgccg acgtggtcga cgcacttgtc ccagccggcg
     2101 gaacagtccg tcccgccgaa cgggtagccc gagccgagcg tgacgagggt cgaggcgcga
     2161 atctcccacg ggagccccac gatgcccgag agcgtgatct ggtgctcctg cgaccccgcg
     2221 ctccgctgcc ggatgccgtt caccgggtcg agaatgcccc agctgaagag gtcgttgccg
     2281 gtctgctccg cgtccgacca gacgtacgag aggaacgctc cccagcccgc ggtggccgag
     2341 aagggcttgt cgaggacgac ctggacggag tcgaaccagg cttccttcga ccgcgagagc
     2401 atcgagaagc cgtagccggg ggcggggcgg gctccccagt tgcacgtccc gtccgcgttc
     2461 ttgatgccgc aggtccaggt cagctggtcc ttgctcttca cgcccgtgta agtgaccgcg
     2521 gcgttgaaga agccgaaatc gtgccgggcg ccgaggctcc actgatccga agagggcggg
     2581 cgggtgtcgt tcgccaggaa gaagagctcg ggctggggcg cttcgccgct cgcgaggagc
     2641 tcctggagcc ccgtcaggct caggtaccgc gggtcccact tgatcgccgg ccggccgtcc
     2701 atcgggccgc cgtcgggcga gaaccagaac gtcgtgacct tgtactggag ccggtacttc
     2761 tcgtcgagga tgtcgttgta gagggtccgg tcgtagtact tgccgtaccc ggcgtggagg
     2821 atcgtcttcc cgtcgccgcg cacgtcccac gagaggccga cgcgcggctg gatcgcgccg
     2881 tagaaggtgt cgcgctgcgt gccgtcggtc aggtagttcg acgggtagat ccccgcgaac
     2941 gccgcgcgga tgtcggccgg cgtctcgtag tcgttgttga gcatgtcggt ctcgaagtcc
     3001 cagcggaggc cgacgttcgc ggtgaagcgg gaggtgatcc gccagtcgtc ctggacatag
     3061 aggccgaact gcgtgttgtt cgtcgagagg tcggggtcgc cgacgccgta gatcgcgttg
     3121 tacggcatgt cgcccggcgt cgtcacgtag gtggagttgc ggaagttgaa gacggggttc
     3181 ccgttcagct gcttgacgac gtggtacttg aggtagtcgt aggtgacgcc gcccttgacg
     3241 acgtggtcgc cgttccacgt caggttcatc agcgtcacgt cgtcccggag ggcgaaccgg
     3301 tcctgcgtga agttctgctg cgtgtccttg ctgcccaccc gcatcaggcc gtcgtagttc
     3361 atccccggga ggctgtcgtc ctgcgggacc gggttccact tgaagcgctg gaagctggcg
     3421 gtcgcctcgt tcaggaacga gccgaggacg gcggtgtgct tgccgcggac cgtccagacg
     3481 tcgttcttga cgttctcggc ggcctggtag ctcgtctggg cgccgaagcc gcggatgtcg
     3541 gtctcgtgcc ggaggtcgcc ggagacgtcg aggtgcgagt tctcggcgag cgaggccgtg
     3601 accttcccga agaggagcgt cgagcggaac gggctcgtgt agagcccctc gtacctcgcg
     3661 aactggtccc ggaacgcggc cggccagttc atgttcgtcc cgcggaagac ctcgtactcg
     3721 cggtcctgga cgttcccctc gtacgaggcg aagaagtgga gcctgtcctt cacgatcggt
     3781 ccgccgaggc tgatgcccgg ctgccagcgc tcgtactccg gcttgaccgc ctcggtgttg
     3841 ttcgcgtcgg cccggcgctg cgtgacgccg tcgatcgcga cgaggtcctt gttctggtag
     3901 tagacgaaga cgtctccgtg gagctcgttg cctcccgact tcgtcacggc cgtgatgatg
     3961 gcgctcgagg ccttctggta ctcggccttg aagttctgcg tgatgacgcg gaactcctgg
     4021 acggcgctct gcgggaacgg gttcccgcgg ctcgcgtcct gcccgaccga cccgccgagg
     4081 aggacgtcgt tcttgtagct cgtcccgtcg atgaagacgt tcgtgttcga cgacccctgc
     4141 gcgccgtagc tgtactcctg ccgcagctcc tcgcgcgaga ggcggacccc cggcgcgagg
     4201 gcggcgaagt tcaggaagtt ccggctcgac tgcgggaggc tctggatctg gatctcgctg
     4261 acgttcgtcg cgacctcgga ggtgacccgc tccacctggg cctcggcggc ggtcgcgacg
     4321 accgcgacct cggccgtgat cgaggccggc tggagcgcga cgtcgagctg gaggctctgg
     4381 ccgacgccga cctggatgct cctcttccac ggagcgtgcg ccccggtgct cacggagagc
     4441 tcgtaggccg ccgcggggag gacgaggacg aaggcgccgt tcgtcgcggt cgccgccgag
     4501 aacctcgccc cggtggcggt gttcacggcg atcacgggca tggaggggat cggcccctgc
     4561 gcgtcggtga ccgttcccct cacctgcgcc gtcgtcgtct ggccggatgc cggcggggcg
     4621 acgagggtcg cgacggcgag gaggacggcc gggagaacga agagggcctt cggccccggg
     4681 ctgcttctca tcgtgccttc ctcctacgat cggttgtcgg ttctggcgtc ggcgcgcccg
     4741 cccgcggagg gcgggacggc tcggtcgtcg tctcggtgtc gattccttcg gtcgagtacc
     4801 ggagaacgag atggaacggc aggacctccc gggccgggag gccgtcgccg cgcccatcga
     4861 tctcggccac gagccgggcg agggcgcgct cgccgagcga acggatgtcg tggcgcaccg
     4921 tggagagcgg cgggctcgcg aaccgcgcga tcgggacgtc gtcgaagccg gcgacggcga
     4981 cgtcggcggg gatcgccgcc cgagcccggc ggagggcgta gagcgcgccg atcgccatag
     5041 agtcgtttgc cgcgaagagg gccgtcgggc ggggagaccg gcggaggagc tcctctcccg
     5101 ccacccgtcc cgactcctcg gcgaagtcgc cctggatctc caggaccgac tcggcggcca
     5161 gcccggccgc ccgagccgcg tcgaacgcgc cccgccgccg ttccgccgcg tcgcggttgg
     5221 agcccggacc gttcaggagc gcgagcctcc ggtgcccgag cgcgaggagg tgggcgacca
     5281 tcgcgcgggc gccgccgcgg ttgtcgacgc tgagggaagg agcgtccgct ccgccggggg
     5341 cggagttcag gaagacggaa ggcagcccgg ccggcatcgc gctcgacaga gcgcgcggcg
     5401 aggcgtgcgg cgccatgacg acgaccccgt cgacccgccc gttcatggcc cggaggacgt
     5461 cgcgggtctc ggcggcgtcg ctatgcgagg aggagacgag gacgtggtag ccggcctgcc
     5521 gggcggcgag gtcgatcccg cggatcacct cggagaagta ctcgccccag atgtcgggga
     5581 ggacgacgcc gaccgtcgac gtccggttcg tgatgaggct gcgcgcggcc gcatgcggcg
     5641 cgtaccgcag ccgggcgatc tcggtctcga cccggcgctt cgtctcgtcc cggacgacgg
     5701 cggtgccgtt cacgacgcgg gagaccgtcg cgacggagac gccagccgcc ctcgcgacct
     5761 cccggattcc gccggtcggg gcgcccgcct cgccggaggt ccggccggcc gatcgggtcc
     5821 cacgcgcgcg tcgctcgtct cgggccatcg ctctgtaacc gttttcatgc gaaaactacc
     5881 cccgacgacg cgtcgatgtc aacaggagat tcgtccgcac gttccgagcc gggtcccacg
     5941 ccataatcga ggcctcgtga accatcgttc cctccgggcc gtcctcgtcg tcctcggtat
     6001 ggccgtcgcg tcctgtcgcg acgtcagggt ccgtccgctc ccggacccgg tccggctcgc
     6061 gcggcagacg tccgtcctct ccctcgaccc gctcgagacc gagccgcgca ccgcctcggc
     6121 cctcgcgaac gtcttcgagc ccctcgtcgc ctacgaccgg aacctcgagc tcgtcccggc
     6181 gctcgccatc cggtgggaga cgcccgacga gaagacttgg gacttcacgc ttcgcccggg
     6241 cgtctccttc cacgacggca cgccgctcga cgcggaggcc gtcgtcgcgg ccctcgatcg
     6301 cgcccggctc agcccggagt ccggcgtccg cgggacgctc tgggccgtct cgcgcgtcga
     6361 ggcgaccgac gaccgtaccg tccgggtcat cactcgcgtt cccgacgccc tcctgctcca
     6421 tgagctaacg ctcgtcctcg tcgcgcgcgg agcgacgcgc gcgcgggtcg aggcgcagcc
     6481 ggtcgggacc ggcccctacc gcgtcgtctc ctggcgaaag ggcgaggcgc tcgagctcga
     6541 ggcgtgggac ggctactggg gcgccgcgcc gagcctccgg aggctccggg tcgttcccct
     6601 gcctcccgac gtcgacggca tcgaggccgt cgcccggggt gaagccgagg tcgccgagct
     6661 ccccgccacc tccgcgcgcc gcacgtcccg gggcgacgtc cggatcgtct cctcgccggg
     6721 actgacgacc ttctacctct ggatgaacgg tccgaggatg atcggcggcc ggcccaaccc
     6781 cttcgccgac gcccgggtcc gccgggccgc cgcgctcgca gtcgaccggg cccgactcgc
     6841 ccgcgcggcc acgggtaccg acgcgacggc ggcgcctcag ctcgttcccc cgacggtcta
     6901 cggtcacgtc cccgcgatga cggcccccgc ccacgacccg gaggcggccc gggccctgct
     6961 gcggtccacc ggtttctcgc tgccggtcga ggcgacgctc gcctaccgcg ccgtcggaac
     7021 cgaagacccc gccgcccggc tcctccagga gatgctcgag acggcgggct tccgcgtcac
     7081 gcttcgtccc gtcccctggg accagatcca ggccgagctg cgcgacgggc ggctctccct
     7141 cttcttcgga gggtgggtct tcgacggccc tgatgcgggc ggtttcctgc gcgactgctt
     7201 ccggagccgc gggccggacg gcctcaccgg cttcttcaac cccggttaca gccaccccga
     7261 gatcgaccgt ctcgtcgatg ccagctacgg cgccttcttc cggacctcgc ggctcctgat
     7321 cctcgaggag acgatgagga ttgcgaacga ggacgccccg ctcgtcccgc tcttccaccg
     7381 gcccgacgcc tgggccgtcg ccccgggcct cctctgggag ccccggcccg acgggaaggt
     7441 cctcgctgcc gagatgcgtc cggccggcgc cccgggctcc tgagcgtcga cacccggcat
     7501 tgcgacccgc tctcgaaccc ctgctcccgg ttccgccgta ctgtctcgtc gaaagaggtc
     7561 tcgaaagatg aacagcgaac gcccccgttc cctcccccgc cgcgtcctct cggccatcgg
     7621 ccgggggctc gccttcgtcc gcaacacctt cctcaacgtc ctcgccctct cgttcgtcgt
     7681 cggcctcctc gccgcgctct tcgccggcgg gccgaaggtc gagaagggct cggccctcgt
     7741 cctcgtcccg aagggcaaca tcgtggagca gctctccgcg aagtccccga gcgacctcgt
     7801 cgagaatctc gcggggggcg gcacggaaga gacgctcctc aaggacgtca tcgacgccct
     7861 cgcggccgcg aagacggacg agaggatctc gtcggtctac ctggacctcg acgagatggg
     7921 aggcgccggg atgacggtcc tggaggacgt cgccgccgcc ctcgccgact tccggtcgtc
     7981 gggaaagaag gtcgtggtcg ccggggacct cttcgacatg cgccgctact tcctcgccgc
     8041 ccagggcgac gagctcctcc tccacccgca agggatggtc ctcatcgagg gcttcggccg
     8101 ctaccggagc tactacaagg acgccatcga ccggctcggc gtcacctgga acgtcttccg
     8161 tgtcggcgag tacaagtcgg ccgtggagcc gttcctgcgg aacgacatgt cccccgaggc
     8221 gaaggaagcc gacctcgagt ggatgggcga cctctggcgg gcgtacctgg cttccgtcgc
     8281 gaaggcgcgg aagacgaccc ccgaggagct cacggccctc atcgagcagc tccccgagcg
     8341 gctgaaggcc tccggcggcg acccggccaa gctggcgctc gacgcgaagc tcgtcgaccg
     8401 gctcgcgacg cgcgacgagg tgcggacgcg gatgatcgag ctggccggcg aagacgcgaa
     8461 gtcgaagtcg ttccggcagg tcgccatggg cgactacctc gaggcgctcg gcagcgaccg
     8521 tcccggcgcg aaggggaagg gggacgcggt cgccgtcgtc gtcgcgaagg gtgagatcgt
     8581 cgacggaacg gccggcccgg gccgcgtggg cggcgactcg acggcgaagc tgatccggca
     8641 ggcccgccag gacgagaagg tcagggcgat cgtcctgcgc gtcgacagcc ccggcgggag
     8701 cgcgttcgcc tccgaggtga tccgccgcga gctccagctg gcgcgcgagg cgaagaagcc
     8761 cgtcgtcgtc tcgatgggct ccgtcgcggc ctcgggcggc tactggatct ccacctcctc
     8821 cgacgagatc tgggcgagcc cggagacgat cacgggctcg atcggcatct tcgggatgtt
     8881 cccgacgatc gaaaagccgc tcgcgaagta cctcggcatc cgcgtcgacg gcgtcgggac
     8941 cacgccgtgg gccggcctcc ggatcgaccg ggagcttccg ccccgggccg gcgaggcgat
     9001 ccaggcgatg atcgaccagg gctacgagga gttcctcgag cgcgtcggga aggcccggaa
     9061 gatgacgcgc gaccaggtcg acaagatcgc ccgcggccgc gtctggagcg gcgccgacgc
     9121 gaaggggatc ggcctcgtcg accacctcgg cggcctcggc aaggcgatcg agtcggcggc
     9181 ggcgaaggcg aagctcggga agagctaccg cgtgacgtac gtggagaagg agaagtcgct
     9241 caaggagaag ctcctctcga gcctctccgt ccgcgcgacg cgcctcgcgg ccgcattcgg
     9301 gtacgccctc gcgccggcgg ccgagaccgc cgacggcccc ggagccgcgc tcctcggcgc
     9361 cgtcgcggcg gaggtcggcc agctctccgt ctggaacgac ccgcgtggcg tctacgccca
     9421 ctgcctctgc gaggtgaagt agcccggcgt ccggtgcgag ccgggcgagc ccgccgctcc
     9481 gatcgatccg aaggccccgc cgcgacgtcg cggcggggcc tttccctttc gggcacgtca
     9541 gcgcgcgagc cggatcccct tcgcgagcgg cccgagctcg acgtcctgcg gcaggtgcga
     9601 tccctccacc cagacgatcc gctggcgaat cgtcgtctcg aggagatcgt ggacggctcc
     9661 ctcgacctcc cgggtgtcgg acatcaggcg ctgcgcgttc caccggaccg cctcccgcga
     9721 gtgctttcgg ccggccgacc agacctggaa cgggacaacg agccggctca ggaacgacat
     9781 cgcctcctcg gccccgagga gaccctcgtc tttcgcgccc ggccccagga tgaggacgac
     9841 cgcccgtcgt ccgttcccct tcgccgcctc gagccccgcg acgaccacgg cgtccgccag
     9901 ccgctgaccg atcggtggct tctcggacaa ggaaaggagg gcgttcat
//
LOCUS       DAOONL010000123         9906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7860_length_9906_cov_16.286773, whole genome shotgun sequence.
ACCESSION   DAOONL010000123 DAOONL010000000
VERSION     DAOONL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7860_length_9906_cov_16.286773"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLP50_14070"
     CDS             <1..599
                     /locus_tag="PLP50_14070"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPA52717.1"
                     /translation="EMGLEHVVLTSVDRDDLPDGGAAHWVRVVEAVKALEPRRAVEVL
                     TPDFQGDEAAIDLVADARPDVFNHNVETVPRLYPTVRPKAVFERSVALLARVKSRHPG
                     TVTKSGLMLGLGEEDAEVLDVFRALRGAGVDVVTVGQYLRPAAWNLPVVSYATPERFE
                     SLRAAGEALGFSYVFAGPFVRSSYRAEEALAESARRLS"
     gene            605..2164
                     /gene="lnt"
                     /locus_tag="PLP50_14075"
     CDS             605..2164
                     /gene="lnt"
                     /locus_tag="PLP50_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPA52718.1"
                     /translation="MEVFSGSLRARAAAALVSGLLLGLTFPNASLVVFLPVAIVPFAL
                     ALDGASPGRGALLGGLFGVAFWLTTFPWIYHVVHRFGGLSAPLASLALLIAACIPSVA
                     FAAMGWAAALAAPRTAAARLLVLPAAWVAQELLRTYAFSGFPWALVSYPLAPWPVLTQ
                     AAALGGAALVSFLVVLVNAALAEAMRERGRGRLAGAAVAGSVLLAAITFGSWRLGRPE
                     ASVGGRSLRVLIVQPNVAQDVRFAPGAAERIHFDVTGLTRALLRKAPADLVLWPESAT
                     PFAWPWSEGLREDLTRLCREERTALLFNTVWSDAPLDDTAAYFNSALLVDGDGVVGEP
                     YHKLRLVPFGEYVPAPSLFGWVNQVSQEAPGAFTPGSRALPLERGGIRLGGAICYEVV
                     YPWIARAQVAAGADALYTLTNDAWYGDGGAQEQHWQAAVFRAVETGRPLLRAAVTGVS
                     GWVDARGRTLARLGPGARAGIAGTLRVPPRGGATLATRLGDAPALVCAAALLVAILRA
                     RASVRRAPPHV"
     gene            2184..3336
                     /gene="prfB"
                     /locus_tag="PLP50_14080"
     CDS             join(2184..2249,2251..3336)
                     /gene="prfB"
                     /locus_tag="PLP50_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152543888.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPA52719.1"
                     /translation="MNEERIEKLTALATQVEALRGYFDVHRAEKEKAELEARAGAPDF
                     WNDAPAAQETLKKLKRADTVLSDDTAFRQKREELEVFAEFLREGEAVEADADAALTSI
                     AAWARERELRFKLTEPEDELPALLEINAGTGGTEAQDWAEMLMRMYLRYGERKGWKTD
                     VLEVSYADEAGIKSATIRFEGEYAYGHMKAEHGVHRLVRISPFDAAARRHTSFASVYV
                     TPEVDDTIEIEILDKDLRIDTFRAGGKGGQHVNKTDSAVRLTHLPTGLVVACQNERSQ
                     HKNKDFAMKVLKSRLYQRAVEEKRAVEEKRAGVKMEIGFGSQIRSYVLAPYRLAKDHR
                     TGFEVGDVDRVLDGDLDGYVEAWLQMAVKKGEKHARERKSADALLEEEA"
     gene            3333..3944
                     /locus_tag="PLP50_14085"
     CDS             3333..3944
                     /locus_tag="PLP50_14085"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPA52720.1"
                     /translation="MTEPAPWILEIRRRLEREWVVPDVVREIAEGTPDARPAAVLVPL
                     YVRERELWTLLTKRSETVESHRGQIAFPGGMEALDDASPWETAIRETEEEIGLPRKAI
                     LRIGELPGVTTFTGFRIRAFVGAIPYPFDVRHDPREVDSVIQVPIRALMGPTLVEERA
                     VTWKGRSIPTPVYHLKGHVIWGATAFLVSSLLEALVDEGRPPA"
     gene            complement(3961..5613)
                     /locus_tag="PLP50_14090"
     CDS             complement(3961..5613)
                     /locus_tag="PLP50_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52721.1"
                     /translation="MTRVRLLLAAAALFALLPALAQPASESLERDARAASRAGKFREA
                     AVKFDQAATAASDPQRRGRLRMQASYAALNAGNATDARESLTAAFADDPALEIVEQLF
                     TPEFRKLWHEVKAEIVKAAPAVLPDLAELKRTSEEKLKDGRFAEVVYDLGNFPHDKLD
                     RDAWALLAQAYERSGRSDQAALARRRALGESVPIAPLPAPAPPSASTLPAGGSSATDL
                     LAFGREALNRGDALTAQAAANRVVEMEPSSSEGYRLLGDSYARRGERALADAMWRQSL
                     RLDERNEGTLLALAGVTLEEKLFDEALDFLRRAVELNPINADRLTTLGRKARSEGDAK
                     MARQVFAAAARALPNDVAVLAEYGAVLFEAGDVDAALEPLMSAASLAPDRAPVRASLA
                     AALRKKGSLPEAEREYREALRADPAWLPALRGLGVLLLATGRPADAVEPLRAALAAAP
                     ADSDTLLALARAQRLSGDLAGAGATLAAASSGHDATLLNEAGAVAYAEGRWGDAQALF
                     EKARAADASLAAAAGNLEKAAAAARVVAALAAAPAPSPAPKR"
     gene            complement(5633..7306)
                     /locus_tag="PLP50_14095"
     CDS             complement(5633..7306)
                     /locus_tag="PLP50_14095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA52722.1"
                     /translation="MLAKVGKYEILEKVGVGGFGTVYKGRDPYIKRTVAIKTCQSDED
                     ELRKRFFREAEFAGNLHHRNITTIYDFGLSDDGLPFIVQEFLTGEDLDRKIKRKDDLP
                     LAWRVRVLADICEGLHYAHTAGIVHRDVKPSNIRILEDGTVKIMDFGIAKSMVSESTL
                     TQTGITLGTASYLAPEQIRGEAVDARTDVFSLGVLAYELLTLTRPFTGDHISTVLYKI
                     MNEPPPSPAEAVPSLPQELVRIVEKALQKDRASRYASCAEMKEDLESVLTVLSGPVGA
                     PPRPPAVDVPPAAGPRGLGSARLDERVLRQAGGPSVAADLPLHTEQSDATPPALPHPE
                     ETGRAALRVFLGALAVLVLVGGGLAWVLLGPGSKQSPLPGPEVDPTATTTAVPVATPS
                     PAPGPTATPPPPTPTPAPTTVPIGTALVSSNVYAHVAIDGRAEGGVLAAKPFRLAAGR
                     HVASFESGGFGPIRVPFEVKAGESVRVRADFPPLGQLWIHVNPDAVGAELFVDGERVG
                     RAGNAPLKKTVAAGDRRVEARLPGFEPAEAIVSVYEQEKEEVRLELRRK"
     gene            7352..8026
                     /locus_tag="PLP50_14100"
     CDS             7352..8026
                     /locus_tag="PLP50_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52723.1"
                     /translation="MKASLAPVLLAAALGAGACRDATPATSPHEPRRTGLSVREAFRP
                     PDDGVLTAQQVETFLKVREATLRIRATPGGNAPLEGEEGISGASEARAAEVRAARSLS
                     VRVDEYFWIRERILEAEAAALTAKLNADVLALLEKTLSSLRDRRPAAPDEASRRLLDE
                     QIASFEAEAARVRREASEKEPESVRANLAVLAPVRSRISAIEDDLAELDATVAREAAP
                     PPASGR"
     gene            8094..8594
                     /locus_tag="PLP50_14105"
     CDS             8094..8594
                     /locus_tag="PLP50_14105"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA52724.1"
                     /translation="MRLIDPLLLEFDRECSGTRKCLERLPADKLAWKPHEKSMSLGKL
                     AGHVATIPGWIGSALLADGFDLASGMATPPLGTPEEILAAFDASVKQAKGAMARLDDA
                     TATGAWTLSKDGQALFSMPRLALVRTILLNHSYHHRGQLTVYLRLLDVPVPGLYGPSA
                     DDDPFK"
     gene            8605..9381
                     /locus_tag="PLP50_14110"
     CDS             8605..9381
                     /locus_tag="PLP50_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPA52725.1"
                     /translation="MPVVLTDLAEGILTVTVNRPEKLNALNAEVIEELRRVFHEAKSN
                     AEVGALVLTGAGEKAFVAGADISGFPSLTPVDARDFARKGQAVLDLLETMGKPSVAAV
                     NGFALGGGCELAMACSMRVAARNARFGQPEVALGLIPGYAGTQRLPRLVGKGRALELI
                     LTGDPIPAEEALRIGLVNRVVEPAELIPTCRALAAKILGKAPLAVRYALEAVNHGLEL
                     PFDEAELHEAALFGLCVATEDMKEGVAAFLGKRPAKFAGK"
     gene            9386..>9906
                     /locus_tag="PLP50_14115"
     CDS             9386..>9906
                     /locus_tag="PLP50_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPA52726.1"
                     /translation="MGGEPRPLGATGLAVPLLGLGAGSVGDGALDEAEAARLLHGALD
                     LGLSFVDTAPGYGLSEERIGRHLSSRRSSFVLSTKLGYGVPGVPDWTPECIARGVDQA
                     LSRLRTDVLDVAHLHSCPLETLLSSGVVEALLDAVRAGKVRVAAYSGENEALAWAVSS
                     GAFGSVQCSVNVC"
BASE COUNT         1273 a         3591 c         3648 g         1394 t
ORIGIN      
        1 ccgagatggg gctcgagcac gtcgtcctca cctcggtcga ccgcgacgac ctccccgacg
       61 gcggggcggc ccactgggtg cgggtcgtcg aagccgtgaa ggcgctcgag ccccgtcggg
      121 cggtcgaggt cctcacgccc gatttccagg gggacgaggc cgcgatcgac ctcgtcgccg
      181 acgcccgtcc cgacgtcttc aaccacaacg tcgagacggt cccgcggctc tacccgaccg
      241 tgcggccgaa ggccgtcttc gagcgttccg tcgcgctcct cgcgcgcgtg aagtcccgcc
      301 atccggggac ggtaacgaaa tcggggctga tgctcggcct cggcgaggag gacgccgagg
      361 tgctcgacgt cttccgcgcg ctgcggggcg cgggcgtgga cgtcgtgacg gtggggcagt
      421 acctccggcc ggcggcctgg aacctcccgg tcgtctccta cgcgacgccc gagcgcttcg
      481 agtcgctccg cgccgcgggc gaagcgctcg gcttctcgta cgtcttcgcc gggccgttcg
      541 tgaggtcgag ctaccgggcc gaggaggcgc tggccgagtc ggcgcgccgc ctctcctgag
      601 cggcatggag gtcttctcgg gctcactccg cgcgcgggcc gcggcggcgc tcgtctccgg
      661 cctcctcctc gggctgacgt ttccgaacgc ctcgctcgtc gtcttcctcc cggtcgcgat
      721 cgtcccgttc gcgctcgcgc tggacggcgc gtcgcccggg cggggcgccc tcctcggcgg
      781 gctcttcggc gtggccttct ggctgacgac gttcccgtgg atctaccacg tcgtccaccg
      841 cttcggcggg ctctcggcgc ctctcgcgtc gctggcgctc ctgatcgccg cctgcatccc
      901 gagcgtcgcg ttcgccgcga tgggctgggc ggcggccctg gccgcgcccc gcaccgcggc
      961 ggcgcgcctc ctcgtcctcc cggccgcctg ggtcgcccag gagctcctcc ggacgtacgc
     1021 cttctccggg ttcccctggg cgctcgtctc ctacccgctc gcgccgtggc cggtcctgac
     1081 gcaggcggcg gccctcggcg gggccgcgct cgtctcgttc ctcgtcgtcc tcgtgaacgc
     1141 cgcgctcgcc gaggcgatgc gggagagggg aaggggccgg ctggccggcg cggcggtcgc
     1201 ggggtcggtc ctcctcgcgg cgatcacgtt cgggagctgg cggctcggcc gcccggaggc
     1261 ctccgtcggc ggacgctccc tccgcgtcct gatcgtccag ccgaacgtcg cgcaggacgt
     1321 gaggttcgcg cccggggccg ccgagcggat ccacttcgac gtcacgggcc tgacgcgcgc
     1381 tctccttcgg aaggcgccgg ccgacctcgt cctctggccg gagagcgcga cgcccttcgc
     1441 ctggccgtgg tcggaggggc tgcgcgagga cctgacgcgg ctctgccggg aggagcggac
     1501 cgcgctcctc ttcaacaccg tctggtccga cgccccgctc gacgacaccg ccgcgtactt
     1561 caactcggcg ctcctcgtcg acggggacgg cgtcgtcggg gagccgtacc acaagctccg
     1621 cctcgtcccg ttcggcgaat acgtgccggc cccctcgctc ttcgggtggg tgaaccaggt
     1681 gagccaggag gcgcctggcg ccttcactcc ggggagccgg gcgctcccgc tcgaacgcgg
     1741 cggcattcgc ctcggcgggg cgatctgcta cgaggtcgtc tacccgtgga tcgcgcgggc
     1801 gcaggtcgcc gccggcgccg acgcgctcta cacgttgacg aacgacgcct ggtacggcga
     1861 cggcggcgcg caggagcagc actggcaggc ggccgtcttc cgggccgtgg agacggggcg
     1921 gccgctcctc cgggcggccg tgacgggcgt cagcggctgg gtcgacgccc gcggccgaac
     1981 gctggcgcgc ctcggcccgg gggcgcgcgc cggtatcgcc ggcacgctcc gcgtcccgcc
     2041 ccgcggcggg gcgacgctcg cgacgcgcct cggcgacgct ccggccctcg tttgcgccgc
     2101 ggcgctcctc gtggctatcc tccgcgcccg ggcgtcggtt cgccgcgcgc cccctcacgt
     2161 ctgaccagga aggacccccg cgcatgaacg aggaacggat cgagaagctg acggcgctcg
     2221 cgacgcaggt cgaggcgctc cgggggtatc tttgacgtcc accgggcgga gaaggagaag
     2281 gcggagctcg aggcgcgcgc cggcgcgccc gacttctgga acgacgcgcc cgcggcccag
     2341 gagacgctga agaagctgaa gagggccgat acggtcctct cggacgacac ggccttccgc
     2401 cagaagcgcg aggagctcga ggtcttcgcg gagttcctcc gggaggggga ggccgtggag
     2461 gcggacgccg acgcggcgct cacctcgatc gcggcgtggg cccgcgagcg cgagctgcgg
     2521 ttcaagctga cggagccgga ggacgagctc ccggcgctcc tcgagatcaa cgccggcacg
     2581 ggcggcaccg aggcccagga ctgggccgag atgctgatgc ggatgtacct ccgctacggc
     2641 gagcggaagg ggtggaagac cgacgtcctg gaggtttcct acgcggacga ggccgggatc
     2701 aagagtgcca cgatccgctt cgagggggag tacgcgtacg gccacatgaa ggcggagcac
     2761 ggcgtccacc gcctcgtgag gatctccccg ttcgacgcgg cggcgcgccg gcacacctcg
     2821 ttcgcctcgg tctacgtcac ccccgaggtg gacgacacga tcgagatcga gatcctcgac
     2881 aaggacctcc ggatcgacac gttccgcgca gggggcaagg gcggccagca cgtcaacaag
     2941 accgactccg cggtccggct gacgcacctc ccgaccggcc tcgtcgtcgc ctgccagaac
     3001 gagcgctccc agcacaagaa caaggacttc gcgatgaagg tcctgaagtc gcgcctctac
     3061 cagcgcgccg tcgaggagaa gcgcgcggtc gaggagaagc gggcgggcgt gaagatggag
     3121 atcggcttcg gctcgcagat ccgctcctac gtcctcgctc cctaccgcct cgcgaaggac
     3181 caccggaccg gcttcgaggt gggggacgtc gaccgggtcc tcgacggcga cctcgacggc
     3241 tacgtcgagg cgtggctgca gatggccgtg aagaaggggg aaaagcacgc ccgcgagagg
     3301 aagagcgcgg acgcgctcct cgaggaggag gcgtgaccga gcccgctccc tggatcctcg
     3361 agatccggcg tcggctcgag cgcgagtggg tcgttcccga cgtggtccgc gagatcgccg
     3421 aagggactcc ggacgcccgg cccgccgcgg tcctcgtccc gctctacgtg agggagaggg
     3481 agctctggac gctcctgacg aagcggagcg agacggtcga gagccaccgc ggacagatcg
     3541 cgttcccggg cgggatggaa gcgctggacg acgcctcgcc gtgggagacg gcgatccgcg
     3601 agacggagga ggagatcggc ctgccgcgaa aggcgatcct gcggatcggc gagctgccgg
     3661 gcgtgacgac cttcaccggc ttccggatcc gtgcgttcgt cggggcgatt ccgtacccgt
     3721 tcgacgtgcg gcacgacccg cgcgaggtcg actccgtcat ccaggtcccg atccgcgcgc
     3781 tgatgggacc gacgctcgtc gaggagcgcg ccgtgacgtg gaaggggcgg tcgatcccga
     3841 cacccgtcta ccacctgaag gggcacgtga tctggggcgc gacggccttc ctcgtctcga
     3901 gcctcctcga ggcgctcgtc gacgaggggc ggcccccggc ctgacggcag accggccgcg
     3961 tcagcgcttc ggcgccgggg agggcgccgg agcggcggcg agggccgcga cgacgcgcgc
     4021 ggcggcagcc gccttctcga ggttcccggc ggcagccgcg agggaggcgt cggccgcgcg
     4081 cgccttctcg aagagggctt gcgcgtctcc ccagcgtcct tccgcgtagg cgaccgcgcc
     4141 cgcctcgttc aggagcgtcg cgtcgtgccc ggaagaggcg gccgccagcg tggctccggc
     4201 gccggcgagg tcgccggaga ggcgctgcgc ccgggccagc gcgaggagcg tgtcggagtc
     4261 ggcgggcgcc gccgccaggg ccgcccggag cggttccacc gcgtcggcgg gccggccggt
     4321 ggcgaggagg aggacgccca gcccgcgcag ggcggggagc cacgcggggt ccgcccgaag
     4381 cgcctcccgg tactctcgct cggcctcggg gagagagccc ttcttccgga gcgcggccgc
     4441 gaggctcgcg cggacggggg cgcggtcggg ggccagcgag gcggccgaca tcagcggttc
     4501 gagagcggcg tcgacgtccc ccgcctcgaa gaggaccgcg ccgtactcgg ccaggaccgc
     4561 gacgtcgttc gggagggccc gggcggccgc cgcgaagacc tgccgggcca tcttcgcgtc
     4621 cccttcgctc cgcgccttcc tgccgagagt cgtcaggcgg tccgcgttga tggggttcag
     4681 ctcgacggcg cggcggagga agtcgagcgc ctcgtcgaac agcttctcct cgagcgtgac
     4741 gccggcgagc gcgaggagcg tcccctcgtt tcgctcgtcg aggcgcagcg actggcgcca
     4801 catcgcgtcg gcgagcgccc tctcacctcg ccgggcatac gagtcgccga ggagacggta
     4861 gccctcgctc gaggagggct ccatctcgac gacgcggttc gcggccgcct gggcggtgag
     4921 ggcgtctccc cggttcagcg cctcgcgtcc gaaggcgagg aggtcggtgg ccgacgagcc
     4981 accggccgga agagtcgaag cgctgggcgg tgcgggggcc ggaagggggg cgatcgggac
     5041 ggactctccg agggcgcggc gccgggcgag ggcggcctgg tcggagcggc cggacctctc
     5101 gtaggcctgc gcgaggagcg cccaggcgtc ccggtcgagc ttgtcgtggg ggaagttgcc
     5161 gaggtcgtag acgacctcgg cgaagcggcc gtccttgagc ttctcctccg acgtccgctt
     5221 cagctccgcg aggtcgggga gcacggcggg ggcggccttc acgatctcgg ccttcacctc
     5281 gtgccagagc ttccggaact cgggggtgaa gagctgctcg acgatctcga gggcggggtc
     5341 gtcggcgaag gccgccgtca ggctctcccg cgcgtccgtc gcgttgccgg cgttcagggc
     5401 ggcgtaggac gcctgcatcc ggaggcggcc ccgccgctgc ggatcggacg cggcggtcgc
     5461 cgcctggtcg aacttcacgg cggcctcgcg gaacttcccg gccctcgagg ccgcgcgcgc
     5521 gtccctctcg agagactcgg acgccggctg cgcgagggcg ggaaggaggg cgaagagcgc
     5581 ggcggcggcg aggaggagtc ggacgcgggt cacgcgccga ttctaaaggc tcctacttcc
     5641 gtcgaagctc caggcggact tcttccttct cctgctcgta gaccgagacg atcgcctccg
     5701 cgggctcgaa cccgggcagc cgcgcctcga cgcgccgatc tccggcggcg accgtcttct
     5761 tgagcggcgc gttgccggcc cgtccgaccc gctctccgtc gacgaagagc tccgccccga
     5821 cggcgtcggg gttcacgtgg atccagagct gtccgagcgg ggggaagtcg gcccggacac
     5881 ggacgctctc gccggccttc acctcgaacg ggacccggat cgggccgaag ccgccgcttt
     5941 cgaacgaggc gacgtgccgg ccggcggcga gccggaacgg cttcgcggcc aggacgccgc
     6001 cctcagcgcg cccgtcgatc gcgacgtggg cgtagacgtt cgaggagacg agcgccgtcc
     6061 cgatcgggac ggtcgtgggc gccggggtcg gcgtgggagg cggtggcgtg gcggtcggtc
     6121 ccggcgcggg cgacggcgtg gccacgggga ccgcggtcgt cgtcgcggtc gggtcgacct
     6181 ccggcccggg gagcgggctc tgcttcgagc cggggccgag gaggacccag gcgaggccgc
     6241 cgccgacgag gacgaggacc gcgagcgccc cgaggaaaac cctgagggcg gcgcgacccg
     6301 tctcctcggg atggggcagc gcgggcggcg tcgcgtccga ctgctcggtg tggagcggca
     6361 gatccgccgc gacggacggg ccgccggcct ggcggaggac cctctcgtcg agtcgcgcgc
     6421 tcccgaggcc ccgcggaccg gccgccggcg ggacgtcgac ggccggcgga cggggaggcg
     6481 cgccgaccgg tcccgaaagg acggtcagga ccgattcgag gtcctccttc atctccgcgc
     6541 aggaggcgta ccggctcgcc cggtctttct ggagcgcctt ctcgacgatc cggacgagct
     6601 cctgcgggag gctcggtacg gcctcggccg gagacggagg tggctcgttc atgatcttgt
     6661 agaggaccgt cgagatgtgg tcccccgtga agggccgggt gagggtcagg agctcgtagg
     6721 ccaggacgcc gagggagaag acgtcggtcc gggcgtcgac ggcctcgccc cggatctgct
     6781 ccggcgccag gtaggacgcc gtcccgagcg tgatcccggt ttgcgtcagc gtcgactcgg
     6841 agaccatcga cttggcgatg ccgaagtcca tgatcttcac ggtcccgtcc tcgaggatcc
     6901 ggatgttgga cggcttcacg tcgcggtgga cgatcccggc cgtgtgcgcg tagtggagcc
     6961 cctcgcagat gtcggccagc acgcggacgc gccaggcgag cgggaggtcg tccttccgct
     7021 tgatcttccg gtcgaggtcc tcgcccgtga ggaactcctg gacgatgaac ggcagcccgt
     7081 cgtcgctcag gccgaagtcg tagatcgtcg tgatgttccg gtggtgcagg ttcccggcga
     7141 actccgcctc gcggaagaac ctcttccgga gctcgtcctc gtcgctctgg caggtcttga
     7201 tggcgacggt ccgcttgatg taagggtccc gtcccttgta gacggtcccg aagccgccca
     7261 cgccgacctt ctcgaggatc tcgtacttgc cgaccttcgc gagcatgccc gtccggcgag
     7321 ggtaacatgc gctcgcgtat attcctctcc cgtgaaggct tcgctcgccc ccgtcctcct
     7381 cgccgccgct ctcggagccg gcgcgtgccg ggacgccact ccggcgactt cgccgcacga
     7441 gccgagacgg acgggcctct ccgtgcgcga ggcgtttcgg cccccggacg acggcgtcct
     7501 cacggctcag caggtggaga cgttcctgaa ggtgcgcgag gcgacgcttc ggatccgggc
     7561 cactccgggc gggaacgcgc cgctcgaggg ggaagagggg atctcgggcg cctcggaggc
     7621 ccgcgccgcc gaggtccggg cggcccgctc gctctcggtc cgcgtcgacg agtacttctg
     7681 gatccgggag cggatcctcg aagccgaggc cgcggcgctg accgcgaagc tgaacgcgga
     7741 cgtcctcgcc ctgctcgaga agacgttgtc gtcgctccgg gaccgccgcc ccgcggcccc
     7801 cgacgaagcc tcgcggcgcc tcctcgacga gcagatcgcg agcttcgagg cggaggcggc
     7861 gcgcgtccgc cgcgaggcct ccgagaagga gcccgagtcg gtccgcgcga acctggcggt
     7921 cctcgccccg gttcgctcgc ggatctcggc catcgaggac gacctcgccg agctggacgc
     7981 caccgtcgcg cgcgaggccg ccccgccccc cgcgtcgggc cgctgaccgc cggacgcgct
     8041 gctaaactcg tcgttcccgt ctccggaatt cgacgatcca gaggagaacg accatgcgtc
     8101 tcatcgatcc gctcctcctc gagttcgacc gcgagtgctc gggcacccgc aagtgcctcg
     8161 agcgcctccc cgccgacaag ctggcgtgga agccgcacga gaagtcgatg agcctcggga
     8221 agctggccgg gcacgtcgcc acgatcccgg gctggatcgg gagcgccctc ctggcggacg
     8281 gcttcgacct ggcgtccggg atggccactc ctccgctcgg caccccggag gagatcctgg
     8341 cggccttcga cgcctccgtg aagcaggcga agggggcgat ggcccggctc gacgacgcga
     8401 cggcgaccgg ggcgtggacc ctctcgaaag acgggcaggc cctcttctcg atgcctcggc
     8461 tcgcgctcgt ccggacgatc ctcctgaatc actcctacca ccaccgcggg cagctcacgg
     8521 tgtacctgcg cctgctcgac gtcccggtcc cgggtctcta cggcccgagc gccgacgacg
     8581 acccgttcaa gtgaggaggc gcgcgtgccc gtcgtcctga ccgacctcgc cgaggggatc
     8641 ctcaccgtca ccgtcaaccg gcccgagaag ctgaacgcgc tgaacgccga ggtgatcgag
     8701 gagctgcgcc gcgtcttcca cgaggcgaag tcgaacgcgg aggtcggcgc cctcgtcctg
     8761 accggagcgg gggagaaggc gttcgtcgcc ggagccgaca tctccggctt cccgtccctc
     8821 acgccggtcg acgcgcgcga cttcgcgcgg aaggggcagg ccgtcctcga cctcctcgag
     8881 acgatgggga agccctccgt cgccgccgtg aacggcttcg ccctcggcgg cggctgcgag
     8941 ctcgcgatgg cctgctcgat gcgcgtcgcc gcgaggaacg cccgcttcgg ccagcccgag
     9001 gtcgccctcg gcctgatccc gggctacgcg gggacgcagc gcctgccgcg cctcgtgggg
     9061 aagggacggg cgctggagct gatcctgacc ggcgacccga tcccggccga ggaggccctc
     9121 cggatcggcc tcgtgaatcg cgtcgtcgag ccggccgagc tgatcccgac gtgccgcgcc
     9181 ctggcggcga agatcctcgg caaggcgccc ctcgccgtcc gctacgccct cgaggcggtg
     9241 aaccacggcc ttgagctccc gttcgacgag gccgagctcc acgaggcggc cctcttcggc
     9301 ctctgcgtcg ccacggaaga catgaaggag ggcgtcgccg cgttcctcgg aaagcgcccg
     9361 gcgaagttcg cggggaagtg accccgtggg cggtgagccc cgcccgctcg gtgcgacggg
     9421 gctcgccgtc ccgctcctcg gtctcggcgc gggttccgtc ggggacggtg ccctcgacga
     9481 ggccgaggcc gctcggctcc tccacggggc gctcgacctc ggcctgtcct tcgtcgacac
     9541 cgcgcccggc tacggcctct ccgaggagcg gatcggccgt cacctctcga gccgacgctc
     9601 ctctttcgtc ctctcgacga agctcggata cggcgtcccc ggcgtccccg actggactcc
     9661 ggagtgcatc gcccgcggcg tcgaccaggc cctctcccgc cttcgcaccg acgtcctcga
     9721 cgtcgcgcac ctccactcct gcccgctcga gacgctgctc tcctcgggcg tcgtcgaagc
     9781 cctcctcgac gccgtccgcg ccggcaaggt ccgcgtcgcc gcctactccg gcgagaacga
     9841 agccctcgcc tgggccgtct cctccggcgc cttcggctcc gtccagtgct cggtcaacgt
     9901 ctgcga
//
LOCUS       DAOONL010000124         9804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_7999_length_9804_cov_15.769617, whole genome shotgun sequence.
ACCESSION   DAOONL010000124 DAOONL010000000
VERSION     DAOONL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_9804_cov_15.769617"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLP50_14120"
     CDS             <1..714
                     /locus_tag="PLP50_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPA52727.1"
                     /translation="VRIASGKTKPVKMTPALVRELLGVPKFRSDEALARDEVGAATGL
                     AWTAVGGDVLIVEARALKGKGRLQLTGQLGDVMKESAQAALTLLRSRADELRLPASFF
                     ESHDVHVHVPEGAIPKDGPSAGVTMLTAMVSAFTGTPVRRDVAMTGEITLRGEVLPIG
                     GVKEKTLAARRAGIRTILLPAQNERDLAEIPEDLRRDLTFRFVDRAEQVLENALVRPA
                     ARRPVARKAARRKTTRRRT"
     gene            711..1748
                     /gene="thiL"
                     /locus_tag="PLP50_14125"
     CDS             711..1748
                     /gene="thiL"
                     /locus_tag="PLP50_14125"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPA52728.1"
                     /translation="MKTLPPETDLLRSFERLFATRRRDVRVGVGDDAAVLAAPSSLAV
                     STDALVEGEDFTGAADPYLVGRKAMNVNLSDLAAMGATPLHALLTLGLPRETPVAWLD
                     GFLDGFRAAAREQAVAIVGGDLTAARERFASVTVLGRVSEEGPLRRGGGGPGDELWVS
                     GTLGAAAAGLSLVLRGYRRDRDGKVTAPRGRRVSPARRDEIARLLRHQLDPRPMVDLG
                     RALAEGGIASAAIDLSDGLARDLHRLCRASGTGAVVETATLPVDSGLPALAELPTSPL
                     DLALFGGEDYGLLFAVPPERLGAMKKLAARFAVRRIGRLDGTSKVLLAEGGAFTSLPD
                     RGFDHFAGPTG"
     gene            1745..2308
                     /locus_tag="PLP50_14130"
     CDS             1745..2308
                     /locus_tag="PLP50_14130"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPA52729.1"
                     /translation="MTFEGRSGESRGGPGDRPPISPIPEEGWWPHFKAQFLEMMGRHQ
                     EPEKVAASFAIGVAIAFTPLIGFHFLMAIVLALLLRLNKLDVVLGTLVVNPLTLGPVS
                     AAALPVGRFLLRARQEAVTHMPWGDLLRPDAWAQAGPAMRTIGIHWGIGMFTFSIVFG
                     ALTYLVLVRVLRRRRDRAALVPPDFAD"
     gene            complement(2346..4118)
                     /locus_tag="PLP50_14135"
     CDS             complement(2346..4118)
                     /locus_tag="PLP50_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52730.1"
                     /translation="MDPLTTPHERAASERARLGVAAAVALALLSVAPYLNALTAGFTF
                     DDGAIVRDNPRLAAPEKVGEVFTTHYFGGPLATGKNYRPVVLLTYAVQRWTTGNEPFP
                     FHLVNLLLHGATTLLFAAWLLALGMPRAPSVAAAALFAVVPIHAEAVTGIVGRAELLV
                     ALLVFLSALLFLRATDGPRLRRIAYTGAVGAFFVALFTKEHAVVLPGVVLLGELLRRG
                     TDEPLAARLRRKVLPAAGLLAPLAAFVAVRALFVGSGLVAKGSAFFELDNPLVLLPAP
                     LRAVNGLWLLLRYAAKTLVPLGLSADHSAHALPLATTLAAPHAVAGLLGAAALAALAA
                     LSVRRLPLVALGLGLFLGAFFPTSNVPFPIGTVFAERLAYLPSAGLLAAVAGGVAALP
                     RTSAGFRRALLAVALVTYAATTVERNEVFRSDETLFADLLVKVPRSARARYNAAYLAW
                     GRGETVVARSNLEKAIALFPRHYDAWALLGLVAAKEGRFDEARAAARESLRIKPDFEI
                     GWRTLAKVEEEAGRPDAAEEALGAGLKRFPRSLPLLRRRAALLLARGRAAESAEAWRT
                     AIAVSGGAREDRLGLERALRAAPR"
     gene            complement(4134..8054)
                     /locus_tag="PLP50_14140"
     CDS             complement(4134..8054)
                     /locus_tag="PLP50_14140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52731.1"
                     /translation="MRGRGRAERGAARPQEWRIPAADAAAGAAEVARLVHSSLPELAW
                     GRSAAGVFVTGPVPAPRPLPGDLSDAEHSAFLVRLAGLLSFLSAHGLGLSAEGAFGLG
                     AKPATRDVPWLVSPPVPAWRSVSAPAALGGVALRLAGREPRFNRQSEARTALAEALSD
                     RLPVRTAEIVAAVLRSVASARPSDALLLDLARACGGTGKVALDLLGLVTPRELDGDSG
                     ERLVTTGPAAEWVARGAARRAPGEVAFAETGSPGSIEEGAPLLALADGLGPDPRAEVL
                     RALARGEPVKALAGPPLVLLARGFDSWGPGAVEAWEAIPSVLPGVVRLETRAEAPPPW
                     QRASLVVPRLGREEVSSLVHLPHASPSAAARIWTELAEEAAGDAGRLLRAARRKARAF
                     LAAGPSRSAPGRASHPESEVVLRASALLAEGFPPEEAAAVSGLGAACVADVLDEAVDE
                     GTLVRTARGLYRFADPGQRQRLAGSLRPVEKEAGIARLEAVCRADARLLLARLAAFGE
                     RPDVVDQARRRLLEAARNGEPEELLALLSRAPASDPDLGAPLLALETFAAAGRMEDAR
                     KAADRIVPRDVASVPLARRVEAARLLARMGRGDQALELVREDGDAEERLARVSLLLRL
                     RREMDADRLLSAIGAAAGGVRGRLLRAELHERRQELDRAETELLAAAEALDGAPEEPG
                     LLDARFTAGYLALALGRPGEARAFFRAALAEARDPSRRADALFDLSVAAAAEGAHADA
                     ERALEEALALFSSLGERDRYVSALGERAALALGRSDAAAAYRDLEVVLARDRQPGRGH
                     QLLFSVPLRQRLALAEGDDADGAEAFAEAAAVPGERPAHPAWREVLVLEGARLLAAGQ
                     AAEALSRLEEAAPLADARSGVEPLRRRLAASALRDLGRPAPLSAELDRDEASLLDAEE
                     RLSRGLNPPAAAWRLLAARLETPGGPVAVTTRLLEWRGRFPGAFSGSGGAALRDLGER
                     AARRAGLGRAAARLAAGAERPGHNGERPAGGSGPGVVAEDAATRELFETARKVAATRL
                     SVLVLGESGTGKEVVAREIHRASGRSGPFVAVNVAALVESLAEAELFGAARGAYTGAD
                     RDRAGVLEASSGGTLFLDEIGDLSPRIQAKLLRVLQEREVRRLGETRTRPVDLRLVTA
                     THRDLARLCTEGRFREDLLYRIAQLSLTLGPLRDRPRDLRALLEAALDGTPLSPEARA
                     AFLSWRWPGNVRELLSAVESARALAGPGERIETTHLPPALRSAPGDAEEKGGRYRAAV
                     DEAKRRVILEALAETGGNRTRAAALLGLSRQSLLYELKRLAIRD"
     gene            complement(8121..9371)
                     /gene="aroA"
                     /locus_tag="PLP50_14145"
     CDS             complement(8121..9371)
                     /gene="aroA"
                     /locus_tag="PLP50_14145"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPA52732.1"
                     /translation="MELPRGRLSGRVRLPGSKSLTNRALLAAALAPGRSELVAPLDCD
                     DTRVLAEALERLGAAIRRGARSWTVEGPLVGAPGAEMTIDVGPAGTPARFLLALLATL
                     PGTFVLDGSPRMRQRPMGPLVEALASRGARIEPLEREGHLPVRIAGRTLSGGAVSIRS
                     DVSSQFVSALLLASPAVAGGLDVRTEGRPASGAYVDLTRQVLAVFAPDGRPRSARYVV
                     PADDSAACFPIAGALVSRGRVELEGLDPGSAQPDAVFRRWAAEAGGALRWEGAGEEAV
                     LAVEGAVRGGVEADVDPAPDAALPLVALVAFAGGASRLRGVARLREKESDRLAAAAEL
                     LTRVGATARVEEEPGEACLLVDGPAGRPARADFRASGDHRVAMAAAVLALALPEGSTL
                     DDPGVVTKSYPGFWSDWSSLVTPL"
     gene            complement(9371..>9804)
                     /locus_tag="PLP50_14150"
     CDS             complement(9371..>9804)
                     /locus_tag="PLP50_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52733.1"
                     /translation="GGRRYAFVHGDRVNTRDLPYRFWRLLSKNPVSRGALALIPGPLA
                     REIVRRTEARLYTTNFRHKKVLPGAELVREGERARAAGYAELLVGHFHVEQHYERTGS
                     VTRVLPAWLEERRHAEIDADGTFRIVTEPSAHRAVRRAPPA"
BASE COUNT         1283 a         3728 c         3717 g         1076 t
ORIGIN      
        1 gtgcggatcg cctcgggcaa gacgaagccc gtgaagatga ccccggcgct cgtccgcgag
       61 ctgctcggcg tgccgaagtt ccgcagcgac gaggcgctcg cgcgggacga ggtcggcgcg
      121 gcgacgggcc tcgcctggac cgccgtcggc ggcgacgtcc tgatcgtcga ggcgcgcgcg
      181 ctgaagggga agggacgcct ccagctgacg ggacagctcg gggacgtgat gaaggagtcg
      241 gcgcaggcgg cgctcactct cctgcgctcc cgcgcggacg agctgcgcct gccggcctcc
      301 ttcttcgaat cgcacgacgt ccacgtccac gtccccgaag gggcgatccc gaaggacggc
      361 ccgtcggccg gcgtcacgat gctcacggcg atggtctccg ccttcaccgg aacgcccgtc
      421 cgccgggacg tcgcgatgac cggcgagatc acgctgcgcg gcgaggtgct cccgatcggc
      481 ggcgtgaagg agaagacgct cgccgcccgg cgggccggga tccggacgat cctcctcccc
      541 gcgcagaacg agcgcgacct cgccgagatc cccgaggacc tccggcgcga cctgacgttc
      601 cggttcgtgg accgcgccga gcaggtcctc gagaacgccc tcgtccgccc cgcggcgcga
      661 cgtccggtcg cccggaaagc ggcgcgaagg aagacgacca ggaggcgaac gtgaagacgc
      721 tcccccccga gaccgacctc cttcgcagct tcgaacggct cttcgcgacg cgccggcgcg
      781 acgtgagggt cggcgtcggg gacgacgccg ccgtcctcgc cgcgccgtcc tcgctcgccg
      841 tctccaccga cgccctcgtg gagggggagg acttcaccgg cgccgccgac ccgtacctcg
      901 tcgggcggaa ggcgatgaac gtgaacctct ccgacctcgc cgcgatggga gcgacgccgc
      961 tccacgcgct cctcacgctc ggcctgccgc gcgagacgcc ggtcgcctgg ctggacggct
     1021 tcctcgacgg ctttcgggcg gccgcgcggg agcaggccgt ggcgatcgtg ggcggcgacc
     1081 tcaccgccgc gcgggagcgg ttcgcctccg tcaccgtcct cggccgcgtc tccgaggagg
     1141 gtcccctgcg ccgcggaggc ggcggcccgg gagacgagct ctgggtctcc gggaccctcg
     1201 gcgccgcagc ggcggggctc tccctcgtcc tgcgcggcta ccgccgggac cgcgacggga
     1261 aggtgaccgc cccgcgcggg cggcgcgtct cgccggcccg gcgcgacgag atcgcgcggc
     1321 tcctcaggca ccagctcgac ccgcgcccga tggtcgacct cggccgggcg cttgccgagg
     1381 gagggatcgc ctcggccgcc atcgacctct ccgacggcct cgcacgcgac ctccaccgcc
     1441 tctgccgcgc gtccggtacc ggcgcggtcg tcgagacggc gacgcttccc gtcgactccg
     1501 gactcccggc tctcgcggag ctgccgacct ccccgctcga cctcgcgctc ttcggcggcg
     1561 aggactacgg gctcctgttc gccgtcccgc cggaacgtct cggcgcgatg aagaagctcg
     1621 ccgcgcgctt cgccgtccgg cggatcgggc ggctggacgg cacgtcgaag gtcctcctcg
     1681 cggagggggg cgccttcacg tccctccccg accgcggctt cgaccacttc gccgggccga
     1741 ccggatgacg ttcgagggac ggagcgggga gagccgcggg ggccccgggg accgcccccc
     1801 gatctcgccg atcccggaag aggggtggtg gccccacttc aaggcgcagt tcctcgagat
     1861 gatggggcgc caccaggagc cggagaaggt cgccgcgtcg ttcgcgatcg gagtcgcgat
     1921 cgcgttcacg ccgctcatcg gattccactt cctgatggcg atcgtcctcg cccttctcct
     1981 gcggctcaac aagctggacg tcgtcctcgg gacgctcgtc gtgaaccccc tgacgctcgg
     2041 cccggtgagc gcggccgcgc tccccgtggg acgcttcctc ctccgagccc gtcaggaagc
     2101 cgtgacccac atgccgtggg gggacctgct ccgccccgac gcctgggccc aggccggccc
     2161 cgcgatgcgg acgatcggga tccactgggg gatcgggatg ttcacgttct cgatcgtctt
     2221 cggcgccctc acctacctcg tcctcgtccg ggtcctccgc cgccgacgcg accgcgccgc
     2281 gctcgtcccg cccgacttcg cggactgact cggccggagg cgcgagccgc ggcccggcga
     2341 cctcctcagc ggggcgcggc ccggagcgcc ctctcgaggc cgaggcggtc ctcccgggcg
     2401 ccgccggaga cggcgatcgc cgtccgccag gcctccgcgg actcggccgc gcggccgcgc
     2461 gcgagaagga gcgcggcgcg gcgacggagg agcgggagcg accgcgggaa gcgcttcagg
     2521 ccggcgccga gcgcctcctc ggccgcgtcg gggcgcccgg cctcctcctc caccttcgcg
     2581 agcgtccgcc agccgatctc gaagtcgggc ttgatccgga gggattcccg tgccgccgcg
     2641 cgcgcctcgt cgaaacgccc ctccttcgcg gcgacgaggc cgaggagcgc ccacgcgtcg
     2701 tagtggcgcg ggaagagggc gatcgccttc tcgaggttcg agcgggcgac gacggtctct
     2761 ccgcggcccc aggcgaggta ggccgcgttg tagcgggccc tcgccgaacg cggcaccttc
     2821 acgagcaggt ccgcgaagag ggtctcgtcg ctacggaaga cctcgttcct ctcgaccgtc
     2881 gtcgcggcat acgtgaccag cgcgaccgcc aggagcgccc gccggaagcc ggcggaggtc
     2941 cggggaaggg cggcgacgcc ccctgcgacg gccgcgagga gccctgccga cggcaggtag
     3001 gcgagacgct cggcgaagac cgtcccgatc gggaacggga cgttcgaggt cgggaagaac
     3061 gcgccgagga agaggccgag accgagcgcg acgagcggga gccggcggac cgagagggcg
     3121 gcgagcgcgg cgagagcggc ggcgccgaga agccccgcga ccgcgtgagg ggccgcgagg
     3181 gtggtcgcga gcgggagcgc gtgcgccgag tggtccgcgg agagtccgag cgggacgaga
     3241 gtcttcgccg cgtagcgcag gaggagccag agaccgttca cggcgcggag cggggccggg
     3301 aggaggacga gggggttgtc gagctcgaag aacgcgctcc ccttcgccac gaggccgctc
     3361 ccgacgaaga gcgcgcggac ggcgacgaag gcggcgagcg gggcgagcag cccggccgcc
     3421 gggaggacct tccgccggag cctcgcggcg agcggctcgt cggtcccgcg ccggaggagc
     3481 tccccgagga ggacgacgcc cggcaggacg acggcgtgct ccttcgtgaa gagggcgacg
     3541 aagaaggctc cgacggcgcc ggtgtacgcg atgcgcctca ggcgcgggcc gtccgtcgcc
     3601 cggaggaaga ggagcgcaga gaggaagacg aggagcgcca cgaggagctc ggcgcgcccg
     3661 acgatcccgg tcaccgcctc ggcgtggatc ggcaccaccg cgaagagggc cgcggccgcc
     3721 acggagggcg cgcgcggcat cccgagcgcg aggagccacg cggcgaagag gagagtggtg
     3781 gcgccgtgca ggaggaggtt gacgagatgg aatggaaacg gttcgtttcc ggtcgtccaa
     3841 cgctggacgg cgtacgtcag gaggacgacg ggcctgtagt tcttcccggt cgcgagcggg
     3901 ccgccgaagt agtgcgtcgt gaagacctcg ccgaccttct cgggcgcggc gaggcggggg
     3961 ttgtcgcgga cgatggcgcc gtcgtcgaac gtgaagccgg ccgtcagggc gttcaggtac
     4021 ggggcgacgg agaggagcgc gagcgcgacc gccgccgcga cgccgaggcg cgctctctcg
     4081 ctggcggcgc gttcatgggg cgtcgtgagc gggtccatcc gggatggcgg agtctagtcc
     4141 cggatcgcga gccgcttcag ctcgtagagg agggactggc gcgagagacc gaggagcgcc
     4201 gcggccctcg tgcggttccc tcccgtctcg gcgagcgcct cgaggatgac tcgcctcttc
     4261 gcctcgtcca cggcggcccg atagcgcccc cctttctctt cggcatcccc gggcgcgctc
     4321 cggagcgccg gcgggagatg ggtcgtctcg attcgctcgc ccgggccggc gagcgcccgg
     4381 gccgactcga cggccgacag gagctcgcgg acgttcccgg gccagcgcca cgagaggaag
     4441 gcggcgcgcg cctccggcga aaggggcgtt ccgtcgagcg ccgcctcgag gagcgcccgg
     4501 aggtcccgcg gcctgtcgcg gaggggtccg agcgtgaggg aaagctgcgc gatccggtag
     4561 agaaggtcct cccggaagcg cccctcggtg cagagtcggg cgagatcgcg gtgcgtcgcc
     4621 gtgacgagtc gcaggtcgac ggggcgggtc cgggtctcgc cgagcctgcg cacctccctc
     4681 tcctgaagga cccgcaggag cttggcctgg atgcgcggcg acaggtcgcc gatctcgtcg
     4741 aggaagagcg tgccgcccga ggacgcctcg aggacgccgg cgcggtcgcg gtcggccccc
     4801 gtgtaggcac cgcgcgccgc cccgaacagc tcggcctccg cgagcgactc cacgagggcg
     4861 gcgacgttca ccgcgacgaa cggaccggat cggccggagg cgcggtggat ctcccgcgcc
     4921 acgacctcct tccccgttcc gctctccccc aggacgagga cggagagccg cgtggccgcg
     4981 accttccggg ccgtctcgaa gagctcgcgg gtcgctgcgt cctccgccac gacgcccggt
     5041 ccgcttccac cggcgggccg ctccccgttg tgcccgggtc gctcggctcc cgcggccagc
     5101 cgcgccgcgg cccggcccag cccggcccgg cgcgccgccc tctcgccgag gtcccggagc
     5161 gcggccccgc cggacccgga gaaggcgccc gggaagcgac cccgccactc gaggaggcgg
     5221 gtcgtcacgg cgaccggccc gcccggcgtc tcgagtcgcg ccgcgaggag acgccaggcg
     5281 gccgcgggag gattcaggcc ccgcgagagc cgctcctcgg cgtcgaggag cgacgcctcg
     5341 tcccggtcga gctctgcgga gagcggcgca ggccgcccga ggtcgcggag cgccgacgcg
     5401 gcgaggcgcc ggcggagagg ctcgactccg ctccgggcgt ccgcgagcgg ggcggcctcc
     5461 tcgagccgcg aaagcgcctc cgccgcctgt cccgcggcga ggagccgcgc cccttcgagg
     5521 acgaggacct ctcgccaggc cgggtgcgcc ggccgctcgc cgggcaccgc ggccgcctcg
     5581 gcgaaggctt cggccccatc ggcgtcgtcg ccctccgcga gcgccagtcg ctgccggagc
     5641 gggaccgaga agaggagctg gtggccacgc cccggctgcc ggtcgcgcgc gaggacgacc
     5701 tccaggtccc ggtacgccgc ggccgcgtcg ctcctcccga gcgcgagggc ggcacgctct
     5761 ccgagcgccg aaacgtagcg gtcccgttcg ccgagcgacg agaagagagc cagcgcttcc
     5821 tcgagcgccc gctcggcgtc cgcgtgcgcc ccttccgccg ccgccgcgac ggagaggtcg
     5881 aagagggcgt cggcacgccg cgacggatct cgcgcctcgg cgagcgcagc gcggaagaac
     5941 gcgcgcgcct ccccgggacg gccgagcgcg agcgcgaggt aaccggccgt gaaccgcgcg
     6001 tcgaggaggc ccggctcctc cggggctccg tcgagggcct cggccgcggc gaggagctcc
     6061 gtctcggccc ggtcgagctc ctgccgccgt tcgtggagct cggcccgaag gagacgcccc
     6121 cgaacccctc ccgcggcggc tccgatcgcg gagaggagcc ggtcggcgtc catctcccgc
     6181 cgaagccgaa ggaggagcga cacacgcgcg agacgctctt ccgcgtcgcc gtcctcccgg
     6241 acgagctcga gcgcctggtc gccccgcccc atcctcgcga ggaggcgtgc cgcctccacc
     6301 ctccgggcga ggggcacgga ggcgacgtcg cgcgggacga ttcgatccgc cgccttccgg
     6361 gcgtcctcca tccgccccgc ggcggcgaac gtctcgaggg cgaggagggg cgccccgagg
     6421 tccgggtccg aggccggcgc ccgggacagg agcgcgagca gctcctccgg ctccccgttc
     6481 cgcgccgcct cgaggagccg ccgccgggcc tggtcgacga cgtcgggccg ctccccgaac
     6541 gcggcgaggc gcgcgaggag aagcctcgcg tcggcccggc agacggcctc gaggcgggcg
     6601 atccccgcct ccttctcgac ggggcggagc gagccggcca gccgctgacg ttgacccggg
     6661 tcggcgaaac ggtagagccc tcgggccgtc cgcacgagcg tcccctcgtc gaccgcttcg
     6721 tcgaggacgt cggccacgca cgccgcgccc aggccggaga cggccgccgc ctcctccggc
     6781 gggaaaccct cagccaggag cgcgctcgcc cgcagcacga cctccgactc gggatgggag
     6841 gcgcggcccg gcgcggagcg ggacggcccc gcggcgagga aggcgcgcgc cttccgcctc
     6901 gcggctcgga ggaggcgccc cgcgtccccg gccgcctctt cggcgagctc cgtccagatc
     6961 cgggccgccg ccgacggcga ggcgtgcggg aggtgaacga ggctcgagac ctcctcgcgg
     7021 ccgagccgcg gcacgacgag gctcgcccgc tgccaaggag gcggagcttc cgcgcgcgtc
     7081 tccagccgga cgacgcccgg gaggacgctc gggatcgcct cccacgcctc gacggctccc
     7141 ggtccccagg agtcgaaacc ccgggccagg aggaccaggg gcggaccggc gagggccttc
     7201 accggctcgc cgcgggcgag cgcccggagg acctcggcgc gcggatcggg cccgagcccg
     7261 tccgcgagcg cgaggagcgg cgccccttcc tcgatcgagc ccggtgatcc ggtctccgcg
     7321 aaggccacct cgccgggagc ccttcgggcc gcgccccgcg cgacccattc ggccgcggga
     7381 cctgtcgtga cgagccgctc gccggagtcc ccgtccagct cgcgcggcgt caccaggccc
     7441 aggaggtcga gggcgacctt ccccgtcccg ccgcaggcgc gtgccaggtc gaggaggagc
     7501 gcgtcggaag ggcgggcgct cgcgacggac cggaggacgg cggcgacgat ctcggccgta
     7561 cggaccggga ggcggtccga gagcgcctcc gccagcgccg tccgggcctc gctctgccgg
     7621 ttgaaccgcg gctcgcgtcc cgccaggcgc agcgcgacgc cgcccagggc cgccggcgcc
     7681 gacacggatc gccacgccgg cacgggagga gacacgagcc acggcacgtc ccgcgtcgcc
     7741 ggcttcgcgc cgagcccgaa cgcgccctcg gccgagagtc cgaggccgtg cgccgagagg
     7801 aacgagagca ggcccgcgag gcgaacgagg aaggcggagt gctcggcgtc cgacaggtcg
     7861 ccggggagcg ggcggggcgc cggtacgggg ccggtgacga agacgcccgc cgcggagcgg
     7921 ccccacgcga gctccgggag cgacgaatgg acgagccgcg ccacctcggc ggctcccgcg
     7981 gcggcgtccg ccgccggaat ccgccattcc tgcggccgcg ccgcacctct ctccgcgcgc
     8041 cctctgcccc gcactcctcc tccggacgaa cgcggaatct tcgcacgatc cggcccgcca
     8101 cgaggcggcg cgagccgtcg tcagaggggc gtgacgagcg agctccagtc gctccagaac
     8161 ccgggatacg acttcgtcac gacgcccggg tcgtcgagcg tcgacccctc gggcagcgcg
     8221 agcgcgagga cggccgccgc catcgcgacc cggtggtccc cggacgcccg aaagtcggcc
     8281 cgcgccggtc tccccgccgg tccgtcgacg aggaggcacg cttcgccggg ctcctcctcg
     8341 acgcgggccg tcgcacccac tcgggtcagg agctcggccg cggccgcgag ccggtccgac
     8401 tccttctccc gcagccgcgc gacgccccga agtcgcgacg ctcctcccgc gaaggcgacg
     8461 agagcgacga gcggcagcgc ggcgtccggc gccgggtcga cgtcggcctc cacgccgccc
     8521 cggaccgcgc cttcgacggc gaggacggcc tcctcgcccg ccccctccca gcggagggcc
     8581 ccgcccgcct ccgcggccca gcgccggaag acggcatcgg gctgcgccga gccgggatcg
     8641 agcccctcga gctccacccg tccgcgcgag acgagggccc cggcgatcgg aaagcaggcg
     8701 gccgagtcgt ccgccggcac cacgtaccgc gcggatcggg gccgcccgtc gggcgcgaag
     8761 accgcgagga cctgccgggt caggtcgacg taagcgcccg acgcggggcg cccctccgtc
     8821 ctcacgtcga gtccgcccgc gaccgccggc gacgcgagga ggagcgcgga gacgaactgc
     8881 gaggagacgt ccgaccggat cgagacggcg ccccccgaaa gggtccggcc cgcgatccgg
     8941 accgggaggt gcccctcgcg ttcgagcggc tcgatccgcg ctccgcgcga ggcgagcgcc
     9001 tccacgagcg gccccatcgg cctctgtctc atccgggggc tcccgtcgag gacgaacgtg
     9061 ccggggagcg tggcgaggag ggcgaggagg aaacgggccg gcgtccccgc cgggccgacg
     9121 tcgatcgtca tctcggcccc gggcgccccg acgagcggcc cctcgacggt ccagctgcgc
     9181 gcgccacgcc ggatcgccgc gccgagccgc tcgagcgcct ccgcgaggac ccgcgtgtcg
     9241 tcgcagtcga gcggggcgac gagctcgctc cgaccgggcg cgagggccgc ggcgaggagg
     9301 gcgcggttcg tgaggctctt ggagccggga aggcggaccc gccccgagag ccggccgcga
     9361 gggagctcca tcacgccgga ggcgccctcc ggacggcgcg gtgcgcgctc ggctcggtca
     9421 cgatccgaaa ggtgccgtcg gcgtcgatct ccgcgtgccg tcgctcctcg agccacgccg
     9481 gcaggacccg cgtgacgctc cccgtccgct cgtagtgctg ctcgacgtgg aagtgtccga
     9541 cgagcagctc cgcgtacccg gcggcccgtg cccgctcgcc ctcgcggacg agctcggcgc
     9601 cggggagcac cttcttgtgc cggaagttcg tcgtgtagag gcgagcctcc gtccggcgga
     9661 cgatttcccg ggcgagcggc cccgggatca gagcgagggc gccgcgcgac acggggttct
     9721 tggacaggag gcgccagaag cgatacggca ggtcgcgcgt gttgacgcgg tcgccgtgga
     9781 cgaaggcgta ccggcgtccg ccga
//
LOCUS       DAOONL010000125         9784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8028_length_9784_cov_19.105561, whole genome shotgun sequence.
ACCESSION   DAOONL010000125 DAOONL010000000
VERSION     DAOONL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8028_length_9784_cov_19.105561"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLP50_14155"
     CDS             <1..561
                     /locus_tag="PLP50_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA52734.1"
                     /translation="KPVGLLVNNAGFGWYGPQAEQGLERTLRMLRLNVSALVELTDRL
                     LVPMRARGAGLVLNVASTAAFLPVPHMSVYAATKAFVLSYSHALHEELAGTGIVVTAL
                     CPGTTRTDFHRVAGLAPGERVRFPSLSAETVAEAGLRGLARGRAVVVPNLLDSAWIFT
                     GRLVPRVVPPKIAAALFSRLKRAPRA"
     gene            complement(580..1479)
                     /locus_tag="PLP50_14160"
     CDS             complement(580..1479)
                     /locus_tag="PLP50_14160"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPA52735.1"
                     /translation="MRRGAALVSLLDEALAGLWRARRMTVVSVLQIAFSLFLVGIFLL
                     AAENLGGVVDALREETAVTVFLRPGTDEAAQADLERRALDSKLVAAVRRVTPEEARRR
                     FARSFSSLASATGTLSANPFPTSVELDLLPGAASSAALRPFLANLTAFPSVEEVQFDV
                     DWVRRLRGVVRLVAVTGAATGFCLALGAAFTIANVVRLTILLHRDEIDILRLVGAPEM
                     LIRGPFLLGGVLQGLLGGLFALGLLGLAFRGVLLFIAATENVLLGVFLVRFLSPTTCG
                     LLVLGGVLAGLFGGTIASRRRHV"
     gene            complement(1476..2213)
                     /gene="ftsE"
                     /locus_tag="PLP50_14165"
     CDS             complement(1476..2213)
                     /gene="ftsE"
                     /locus_tag="PLP50_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPA52736.1"
                     /translation="MIEFYGVSKEYHGRRVLKNLSFQVAQGEFIFLTGPSGAGKTTVL
                     KLLYRAETPGAGQILVNGRNVVSMPASRVPELRRTMGIVFQDYKLLPTRTVFENVSFV
                     LKFLGVSLAERRRRAYQVLRLVELHHRVNAFPEELSGGEQQRVALARALVNEPELLVA
                     DEPTGNLDHRLAQEMMRLFSQINVRGTTVLVATHDVALIRETGRRCLTLDRGGILEGL
                     VEAALPPQATWRREGSSAHPVVPEERP"
     gene            complement(2210..3670)
                     /gene="gatB"
                     /locus_tag="PLP50_14170"
     CDS             complement(2210..3670)
                     /gene="gatB"
                     /locus_tag="PLP50_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPA52737.1"
                     /translation="MLPHDLEAVIGLEVHVQLRTRTKAFCACPVVFAAAPNSAVCPVC
                     LGYPGALPVPNREMALLGARLAIATGCDVHARSAFARKSYFYPDLPKGYQITQLDRPL
                     ATGGRVDVRAADGSTRSVRLRRIHLEEDAGKSIHDAEGDDVPPGVTLVDLNRAGTPLA
                     EIVTEPDLRAPAEASDFLSRLRRLVRWIGVSDGNMEAGSLRCDANVSVRPAGTETLGT
                     KVEIKNLNSITHVRMALEHEVARQAGVLRAGGAVAAESRLFDPASGTTRPMRGKEETE
                     DYRYFPDPDLGPLVLDEAWLEEVAASLPEPPEAREARFVAELGLSPADAATLCATRPL
                     ADAFEEAVALLPSNARGVAAWFLGDLLARMGDSDRQVGRLPVPPGEVARLVARIDDGT
                     ISGRIARELFALLCAGARDVDGLIRERGLVQVTDETPIRAAVDAVLAASEAQAAAYRG
                     GKSSTFGWFVGQVMKATGGKASPAVVNRLLRERLDA"
     gene            3757..4719
                     /locus_tag="PLP50_14175"
     CDS             3757..4719
                     /locus_tag="PLP50_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA52738.1"
                     /translation="MTRARASRTPAAGGRPGGSPVVSRLTHATFRLWFPFFRWLVTAL
                     PPEWVARLAAATAERAIWARESVREAILENHAAVLGLPPWRREVEESARAMLGHHSRS
                     WIDLLRFSAHPPADLDAIVPERIGTEHLVAARDAGRGAILLTAHVGNFEIGGFFLQEI
                     GLRVAVVYLPDPSPVVERDRTAARDRLGIRSIPITSELSAVKVLRSLEEGYFVAIQGD
                     RDYGGTGRRMPFLGREVSFPVGPFRIAAAAGAPLLPVFVLRGEGGTYRTVVEAPLRVE
                     AARRGERDEAERRAMTDFVAILERTIRENHAQWYTFTRFWEPPT"
     gene            4798..5637
                     /locus_tag="PLP50_14180"
     CDS             4798..5637
                     /locus_tag="PLP50_14180"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPA52739.1"
                     /translation="MKIVLAIDPAQPLAAYRDALLAAGARDEEVVVVQPGDALPAEFD
                     GLLVSGGADVDPARYGETKVNETVTPRPERDTLDFALVERAGALGAPIFGICRGLQVL
                     NVALGGTLWQDLPSQRDRGVSHAFSTRDGHDPAHLAHVVHARRNVSGRLPLAAEIAAL
                     DGEPVNSRHHQAVKDLAPGLVPLAASPDDLLEAFARDGGAFLAAVQWHPENLVGRPVQ
                     KALFTSFLAAVRRRAALVNPHASPGDRAVGAASERGGQHLAPGDPGGRSDVRVASGAP
                     EPR"
     gene            5634..6611
                     /locus_tag="PLP50_14185"
     CDS             5634..6611
                     /locus_tag="PLP50_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA52740.1"
                     /translation="MTKSRLALLALLLLVAVPAELAVAQAGATPSGPPPLPKDVPAFQ
                     VGQYNAFTWYGRFGYTNLSFIDTGDGVLVIDTGWTKQDGENLKAQIAEKTNGKPVRWI
                     VMTQTDVDSNGGIAAFLPTDATIFVHARALDPLSAGVLRAEPGQKKPTIVGVADHLVL
                     NAGGRRFELLAAPGRAHSEYDLVLYNNDSGLVWAGDLITPGRCPNLTNPVSDPNAWIE
                     MIGRIQSLGAVGIVPTRGDATKAVADEIEKSRAYIERVLRFVAEQKVKNSPEARVAAE
                     LSLKKIGDYCPLTGDNANVMALYRRMQPDGTFRLPGAVAPARPAAPAPR"
     gene            complement(6841..7239)
                     /gene="queF"
                     /locus_tag="PLP50_14190"
     CDS             complement(6841..7239)
                     /gene="queF"
                     /locus_tag="PLP50_14190"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPA52741.1"
                     /translation="MTTGYTDDHAASGTKAELPSLETWPSQYADDYEVTITVPEFTSI
                     CPKTGLPDFATLVVTYVPEAACVELKSFKEYLLAYRNLGIFNENVVNRVLADLVAAMR
                     PRRMSVRGIFTTRGGIQTTVEARWPKPARP"
     gene            7296..8024
                     /gene="lepB"
                     /locus_tag="PLP50_14195"
     CDS             7296..8024
                     /gene="lepB"
                     /locus_tag="PLP50_14195"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPA52742.1"
                     /translation="MAISPEKRKQILKDVRQLVLMAAIILTGRSVLADWYVVPTGSMK
                     PTILEGDRVFVWKSAYQIRVPFSKIRIMTTGEPRRGDVVVVRNPDGGSVPFVKRLIGL
                     PGDVVELRNEALLVNGKPQKVEYLEPLTLPDGDIEILGTERIDGRTHPIRILPERHAL
                     RNFGPITVPEDEVFLMGDNRDESRDARFFGTRPVVDLLGRAVGVMWSWSPARADGSAL
                     SRLGRPRFSRFGRAFITSAPAARE"
     gene            8145..8591
                     /locus_tag="PLP50_14200"
     CDS             8145..8591
                     /locus_tag="PLP50_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPA52743.1"
                     /translation="MTEPRLAVLAFLAGTDRHPTAEEVESAVNREGPVLSRASVYNVL
                     HALVDVGLVTGLSVKDGPVRYDAHLGPHHHLVCRGCGRVEDVEWKDFAVAERGVLPDG
                     RAVRPLFLTLDGLCTGCREKEEGRGEPASPLPSRQPGNAQRFSTGT"
     gene            complement(8567..>9784)
                     /locus_tag="PLP50_14205"
     CDS             complement(8567..>9784)
                     /locus_tag="PLP50_14205"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPA52744.1"
                     /translation="IKAMDLRKIKVNPEDFGLMTYDPAFLNTASCKSKITFIDGDKGI
                     LEYRGYPIEQLAEKSSFLEVAWLLWNGELPTKTELDEFTRHVTHHTYLHENIKKLMEG
                     FRHDAHPMGMLQSTVGALSTFYPTAKQVNDPRQRAHHTIRLIAKMPTIAAFAYRHSIG
                     FPYVYPDNELSYVGNFLSMLRKMSEPKYKVNPVLERALDVLFILHADHEQNCSTNAMR
                     AISSAQTDPYSSLSGAISALYGPLHGGANEAVLTMLREIGTINNVPAFVKEVKEGQGE
                     KRLMGFGHRVYKNYDPRAKVIKALAEQVFEVTGKNPLLTIAVELERIALQDDYFVKRK
                     LYPNVDFYSGLIYEAMGFPPDYFTVLFAIGRTAGWLAQWHEMVNDPDQKIARPRQVFL
                     GPARRDYVPVEKR"
BASE COUNT         1383 a         3441 c         3536 g         1424 t
ORIGIN      
        1 aagccggtcg gcctcctcgt gaacaacgcg ggcttcggct ggtacgggcc gcaggccgag
       61 caggggctcg agcggaccct ccggatgctc cggctgaacg tgtccgcgct cgtcgagctg
      121 accgaccggc tcctcgtccc gatgcgcgcg cgcggagcgg ggctcgtcct gaacgtggcg
      181 tcgacggccg cgttcctccc cgtcccgcac atgtcggtct acgcggcgac gaaggcgttc
      241 gtcctctcct actcccacgc gctccacgag gagctcgcgg ggacggggat cgtcgtcacg
      301 gcgctctgcc ccgggacgac gcggaccgac ttccaccgcg tcgccggcct cgcgccgggg
      361 gagcgggtcc gcttcccgtc gctctcggcg gagacggtcg ccgaggcggg gctccgcggc
      421 ctggcgcgcg gccgggccgt cgtcgtgccg aacctcctcg actccgcctg gatcttcacg
      481 gggcgcctcg tgccgcgcgt ggtcccgccg aagatcgcgg ccgccctctt ctcgcgcctg
      541 aagcgcgcgc cgcgcgcctg agcctcgccg gcgccggcct cagacgtgcc ggcgccgcga
      601 ggcgatcgtc ccgccgaaga gccccgcgag gacccctccg aggacgagga ggccgcacgt
      661 cgtcggagag aggaaccgga cgaggaagac gccgaggagg acgttctccg tcgcggcgat
      721 gaacaggagg acgccccgga acgcgaggcc gagcaggccg agcgcgaaaa gcccgccgag
      781 gagcccctgg aggacgccgc cgaggaggaa cggtccgcgg atgagcatct ccggggcgcc
      841 gacgaggcgg aggatgtcga tctcgtcgcg gtggaggagg atcgtcagcc ggacgacgtt
      901 cgcgatcgtg aacgcggcgc cgagcgcgag gcagaagccg gtggccgccc ccgtcaccgc
      961 gacgagccgg acgacgccgc gtaggcgcct cacccagtcg acgtcgaact gcacctcttc
     1021 gacggagggg aaggccgtga ggttcgcgag gaaggggcgg agcgcggcgg aggacgcggc
     1081 gcccggcagg aggtcgagct cgaccgaggt ggggaagggg ttcgcggaga gcgtcccggt
     1141 cgcgctggcg aggctcgaga acgagcgggc gaagcggcgc cgcgcttcct cgggcgtgac
     1201 gcgacggacg gcggcgacga gcttcgagtc cagcgcgcgg cgctcgaggt ccgcctgcgc
     1261 cgcctcgtcg gtgccggggc gcaggaagac ggtgacggcc gtctcctcgc ggagcgcgtc
     1321 gacgacgccg ccgaggttct cggcggcgag gaggaagatc ccgacgagga agagcgagaa
     1381 cgcgatctgg aggacggaga cgacggtcat ccggcgggcg cgccagaggc cggcgagcgc
     1441 ctcgtccagc agcgagacga gggcggcgcc gcgcctcacg gccgctcctc ggggacgacg
     1501 gggtgtgccg acgagccctc gcgccgccac gtcgcctgcg gagggagcgc cgcctcgacg
     1561 agcccttcga ggatcccgcc ccggtcgagc gtgaggcatc ggcggcccgt ctcgcggatc
     1621 agcgcgacgt cgtgcgtcgc gacgaggacg gtcgtcccgc ggacgttgat ctgcgagaag
     1681 agccgcatca tctcctgcgc gaggcggtgg tcgaggttcc cggtcggctc gtcggcgacg
     1741 aggagctcgg gctcgttcac gagggcgcgc gccagcgcga cccgctgctg ctcgccgccg
     1801 gagagctcct cggggaacgc gttgacgcgg tggtggagct cgacgagccg gaggacctgg
     1861 taggccctgc gcctccgctc ggccagggag actccgagga acttcaggac gaacgagacg
     1921 ttctcgaaga cggtgcgcgt cgggaggagc ttgtagtcct ggaagacgat ccccatcgtc
     1981 cggcggagct ccgggacgcg cgacgccggc atggagacga cgttccgccc gttgacgagg
     2041 atctgtccgg cgcccggcgt ctcggcgcgg tagaggagct tgaggacggt cgtcttcccg
     2101 gctcccgagg gaccggtgag gaagatgaac tctccctgcg ccacctggaa cgagaggttc
     2161 ttcaggaccc gccgcccgtg gtactccttc gagacgccgt agaactcgat cacgcgtcga
     2221 gcctttcccg gaggaggcgg ttcacgacgg cggggctcgc cttcccgccg gtcgccttca
     2281 tcacctggcc gacgaaccag ccgaacgtcg agctcttccc cccgcggtag gcggcggcct
     2341 gcgcctcgct cgcggcgagg acggcgtcga cggcggcgcg gatcggcgtc tcgtccgtca
     2401 cctggacgag ccccctctcg cggatcagcc cgtcgacgtc ccgcgcgccc gcgcagagga
     2461 gcgcgaagag ctcccgggcg atccgccccg agatcgtccc gtcgtcgatc cgcgccacga
     2521 gccgggcgac ctcgcccggc gggacgggga gccggcccac ctgccgatcg gagtctccca
     2581 tccgcgcgag gaggtcgccg aggaaccacg cggcgacgcc gcgggcgttc gacgggagga
     2641 gcgcgaccgc ctcctcgaac gcgtcggcga gcggtcgcgt cgcgcagagc gtcgcggcgt
     2701 cggccgggga gagaccgagc tcggcgacga agcgcgcctc gcgcgcctcc ggcggctcgg
     2761 ggagagaggc cgcgacctcc tcgagccacg cctcgtcgag gacgaggggt ccgaggtccg
     2821 ggtcggggaa atagcggtag tcctccgtct cctccttgcc gcgcatcggg cgcgtcgtgc
     2881 cggacgcggg atcgaagagg cgggactcgg cggcgacggc gccgccggcg cgaaggacgc
     2941 cggcctggcg cgcgacctcg tgctcgagcg ccatccgcac gtgggtgatc gagttgaggt
     3001 tcttgatctc gaccttcgtc ccgagcgtct cggtgcccgc gggcctcacg gagacgttcg
     3061 cgtcgcagcg gagggagccc gcctccatgt tcccgtccga gacgccgatc caccggacga
     3121 gccgccggag acgggagagg aagtcggacg cctcggcggg agcgcgcagg tcgggctcgg
     3181 tgacgatctc ggcgagcggg gtcccggccc ggttcaggtc gacgagggtg acgccgggcg
     3241 ggacgtcgtc gccctcggcg tcgtggatcg acttgcccgc gtcctcctcg aggtggatcc
     3301 gacggaggcg gacggagcgg gtcgagccgt ccgcggcgcg cacgtcgacc cggcctcccg
     3361 tcgcgagcgg gcggtcgagc tgggtgatct ggtatccctt cgggaggtcg gggtagaagt
     3421 agctcttccg ggcgaaggcg gagcgcgcgt gcacgtcgca ccccgtggcg atcgcgaggc
     3481 gcgccccgag gagcgccatc tcccggttcg gcaccggaag ggcccccggg tagccgaggc
     3541 agaccgggca gacggcgctg ttcggcgcgg ccgcgaagac caccgggcag gcgcagaacg
     3601 ccttggtccg cgtcctcagc tgaacgtgga cctccagccc gatgaccgcc tcgagatcgt
     3661 gagggagcat tccggacggc gagtctagcc gagcgtgccg ccgcctctcc ctgcggctag
     3721 aatcggcggc cccgggcgcc gcgaggcgtc cctcccatga cacgagcccg cgcttcccga
     3781 acgcccgccg cgggcggccg ccccggcgga tcgcccgtcg tctcgcgtct gacgcacgcg
     3841 acgttccgcc tctggttccc gttcttccgc tggctcgtga cggcgcttcc ccccgagtgg
     3901 gtcgcgcgcc tggcggccgc gacggcggag cgggcgatct gggcgcgcga gagcgttcgc
     3961 gaggcgatcc tcgagaacca cgcggcggtc ctcggcctcc cgccgtggcg gcgcgaggtg
     4021 gaggagtcgg cccgcgcgat gctggggcac cactcgcgct cctggatcga cctcctccgc
     4081 ttctcggcgc accccccggc cgatctcgac gcgatcgtcc cggagagaat cggcaccgag
     4141 cacctcgtcg ccgcgcgcga cgcggggcgg ggcgcgatcc tcctgaccgc ccacgtcggc
     4201 aacttcgaga tcggcggctt cttcctgcag gagatcggtc tccgggtcgc ggtcgtctac
     4261 ctgcccgacc cgtcgcccgt cgtggagcga gaccgcaccg cggcgcgcga ccgcctcggc
     4321 atccgctcga tcccgatcac gtcggagctg tcggccgtga aggtgctgcg gagcctcgag
     4381 gaggggtact tcgtcgcgat ccagggagac cgcgactacg gcgggacggg tcggaggatg
     4441 ccgttcctcg ggcgcgaggt ctcctttccc gtcgggccgt tccggatcgc cgcggctgcc
     4501 ggagcccccc tcctgcccgt cttcgtcctt cgcggagagg gagggacgta ccggacggtc
     4561 gtcgaggcgc ctctccgcgt cgaggcggcg cggagaggcg agcgagacga ggccgagcgc
     4621 cgggccatga ccgacttcgt tgcgatcctg gagcggacga tccgggagaa ccacgcccag
     4681 tggtacacct tcacccgctt ctgggagccg ccgacctgag acgggcgcgc gcctggaacg
     4741 ggccttgcta gactccgccg ccgccggcga ccgccgccgg atcggccctc ccgcgtgatg
     4801 aagatcgtcc tcgccatcga ccccgctcag ccgctcgcgg cctatcgcga cgccctcctc
     4861 gccgctggcg cgcgggacga ggaggtcgtg gtcgtgcagc cgggggacgc gcttcccgcg
     4921 gagttcgacg gcctcctcgt ctcgggcggg gcggacgtcg acccggcgcg ctacggggag
     4981 acgaaggtaa acgaaacggt gacgccgcga ccggagcggg acacgctcga cttcgcgctc
     5041 gtggagcggg cgggggcgct cggggctccc atcttcggga tctgccgcgg gcttcaggtc
     5101 ctgaacgtcg cgctcggcgg gacgctctgg caggacctgc cctctcagcg cgatcggggc
     5161 gtctcccacg cgttctcgac gcgggacggg cacgacccgg cgcacctcgc gcacgtcgtc
     5221 cacgcccgca ggaacgtctc cgggcggctg ccgctcgccg cggagatcgc ggcgctcgac
     5281 ggcgagccgg tcaactcccg ccaccaccag gccgtgaagg acctcgcccc cggcctcgtc
     5341 cccctcgcgg cctcccccga cgacctcctc gaggcgttcg cgcgcgacgg gggcgcgttc
     5401 ctcgccgccg tccagtggca cccggagaac ctcgtcggcc gaccggtcca gaaggccctc
     5461 ttcacctcgt tcctcgccgc ggtccgccgt cgggcggccc tcgtgaaccc tcacgcctct
     5521 cccggagatc gcgccgtcgg ggccgcgtcc gagcgtggag gccagcacct cgcgcccgga
     5581 gatccgggcg gacgttccga cgtccgcgtg gcctccgggg cgccggagcc aagatgacga
     5641 aatcccgcct cgcccttctc gccctgctgc tcctcgtcgc cgtccccgcg gagctcgcgg
     5701 tcgcccaggc cggcgccacg ccgtccgggc cgccgccgct tccgaaggac gtccccgcgt
     5761 tccaggtggg gcagtacaac gccttcacct ggtacgggcg cttcggctac acgaacctgt
     5821 cgttcatcga cacgggcgac ggcgtcctcg tcatcgacac ggggtggacg aagcaggacg
     5881 gcgagaacct gaaggcgcag atcgcggaga agacgaacgg gaagccggtc cgctggatcg
     5941 tcatgacgca gacggacgtc gactcgaacg gcgggatcgc ggccttcctc ccgaccgacg
     6001 cgacgatctt cgtccatgcc cgcgcgctcg atccgctctc cgcgggcgtc ctgcgggccg
     6061 agccgggaca gaagaagccg acgatcgtcg gcgtggcgga ccacctcgtc ctgaacgccg
     6121 gcgggcgccg gttcgagctc ctcgccgcgc ccgggcgcgc ccactccgag tacgacctcg
     6181 tcctctacaa caacgacagc ggcctcgtct gggcagggga cctcatcacg ccggggcgct
     6241 gcccgaacct gacgaacccc gtctccgacc cgaacgcctg gatcgagatg atcggccgga
     6301 tccagtcgct cggcgccgtc gggatcgttc cgacgcgcgg ggacgcgacg aaggccgtcg
     6361 ccgacgagat cgagaagagc cgcgcctaca tcgagcgggt cctccgcttc gtcgccgagc
     6421 agaaggtgaa gaactcccct gaggcgcgcg tggcggcgga gctctcgctg aagaagatcg
     6481 gcgactactg cccgctcacc ggcgacaacg cgaacgtcat ggccctctac cggcggatgc
     6541 agcccgacgg aaccttccgg cttccgggtg cggtcgctcc cgcgcgtccc gccgccccgg
     6601 ccccgcgctg aaacggtagg cccggaaacg agaatcccgg ccggttgatc acaccggccg
     6661 ggacgggacg ggagggcgtc gggtcctccg aactcacggt cgccctcttc cttttgagcc
     6721 gcgtccgcgg cccgtctttc cttcgcgggg acgatccggt gtcgccccgg aaagaacctc
     6781 gttaccgctg gaaagagctt tcgtgtcccg tggaaaacat gggtttcgga acgcccgatg
     6841 tcaagggcgc gcgggcttcg gccagcgcgc ttcgaccgtc gtctggatgc cgccgcgcgt
     6901 cgtgaagatg ccccgcacgg acatcctgcg cggtcgcatc gcggcgacga ggtcggcgag
     6961 gacgcggttc acgacgttct cgttgaagat gccgaggttc cggtaggcca ggaggtactc
     7021 cttgaaggac ttcagctcca cgcacgccgc ttcggggacg tacgtgacga cgagcgtcgc
     7081 gaagtcgggg aggccggtct tcggacagat cgaggtgaac tccgggaccg tgatcgtcac
     7141 ctcgtagtcg tccgcgtact gcgagggcca tgtctcgagg gacgggagct ccgctttcgt
     7201 ccccgaggcg gcgtggtcgt ccgtgtagcc ggtcgtcatg gcggaaggat agcccggccg
     7261 tcccgcggca cggaatgtgc gacactccgg ggccgatggc catctcgccc gagaaacgca
     7321 agcagatcct gaaggacgtg aggcagctcg tcctcatggc ggcgatcatc ctgaccggcc
     7381 gctccgtcct cgccgactgg tacgtcgttc cgaccggttc gatgaagccg acgatcctgg
     7441 agggggaccg cgtcttcgtc tggaagtccg cctaccagat ccgtgtcccg ttctcgaaga
     7501 tccggatcat gacgaccggc gagccgcggc ggggagacgt cgtcgtcgtg cgcaacccgg
     7561 acggcgggag cgtccccttc gtcaagaggc tgatcggtct cccgggcgac gtcgtcgagc
     7621 tgcggaacga ggcgctcctc gtgaacggaa agccccagaa ggtggaatac ctcgagccgc
     7681 tcacgctgcc ggacggcgac atcgagatcc tcggcaccga gcggatcgac ggccggacgc
     7741 acccgatccg gatcctgccg gaacgccacg ccctccggaa cttcggtccg atcacggtcc
     7801 cggaggatga ggtcttcctg atgggagaca accgcgacga gagccgcgac gcccgcttct
     7861 tcgggacgcg gccggtcgtc gacctcctcg ggagagccgt cggcgtcatg tggagctgga
     7921 gccccgcccg ggccgacgga tcggctctct cccgtctcgg ccggccccgt ttctcgcgct
     7981 tcggacgagc cttcatcacg tccgctcccg ccgcccggga gtaggcccgc cgtccggctc
     8041 cggggcggag ccgcttgcca tcgccgtcgt ttggatctat tctaaatagt gtgaagatgg
     8101 ctccgcccgc cctggaatgc ctgctccgcg cccgagggct tcgggtgacc gagccccggc
     8161 tggcggtgct tgccttcctc gccgggaccg accgtcaccc gaccgccgag gaggtcgaga
     8221 gcgccgtgaa ccgggagggg ccggtccttt cgcgcgcctc ggtctacaac gtcctccatg
     8281 cgctcgtgga cgtcgggctc gtgacgggcc tcagcgtgaa ggacgggccg gtccgctacg
     8341 acgcccacct cggaccgcac caccacctcg tctgccgggg ctgcggccgg gtcgaggacg
     8401 tcgagtggaa ggacttcgcg gtggccgagc gtggcgtcct tcccgacggc cgcgcggtac
     8461 gccccctctt cctgaccctg gacgggctct gcaccggatg ccgcgaaaag gaagagggga
     8521 gaggcgagcc cgcctccccc ctcccttccc ggcaacccgg gaacgctcag cgcttctcga
     8581 cgggaacgta gtcgcgccgg gccggtccga ggaagacctg gcgcggccgg gcgatcttct
     8641 ggtcggggtc gttgaccatc tcgtgccact gtgcgagcca gccggccgtc cggccgatcg
     8701 cgaagaggac ggtgaagtag tcggggggga agcccatcgc ctcgtagatg aggcccgagt
     8761 agaagtcgac gttcgggtag agcttcctct tgacgaagta gtcgtcctgg agcgcgatcc
     8821 gctcgagctc gaccgcgatc gtcaggagag ggttcttccc cgtgacctcg aagacctgct
     8881 cggcgagcgc cttgatgacc ttcgcgcgcg ggtcgtagtt cttgtagacc cgatgcccga
     8941 agcccatcag cctcttctcg ccctgtccct ccttgacctc cttgacgaag gccgggacgt
     9001 tgttgatggt cccgatctcg cgaagcatcg tgaggacggc ctcgttcgcg cctccgtgga
     9061 gcggcccgta gagcgcggag atggcgccgg agaggctgga gtacgggtcg gtctgcgcgg
     9121 agctgatcgc gcgcatcgcg ttcgtcgagc agttctgctc gtggtccgcg tggaggatga
     9181 agaggacgtc gagcgcccgc tcgaggaccg ggttcacctt gtacttcggc tccgacatct
     9241 tccggagcat cgagaggaag ttgccgacgt aggagagctc gttgtcgggg tagacgtagg
     9301 ggaagccgat ggagtgccgg taggcgaacg cggcgatcgt cggcatcttc gcgatgaggc
     9361 ggatcgtgtg gtgcgcccgc tggcgcgggt cgttcacctg cttcgcggtg gggtagaacg
     9421 tcgagagcgc gccgacggtc gactggagca tccccatcgg atgcgcgtcg tggcggaacc
     9481 cctccatgag cttcttgatg ttctcgtgga ggtaggtgtg gtgcgtgacg tgccgcgtga
     9541 actcgtcgag ctcggtcttc gtcggcagct cgccgttcca gaggagccac gcgacctcga
     9601 ggaagctcga cttctcggcg agctgctcga tcgggtaacc gcggtactcg aggatcccct
     9661 tgtcgccgtc gatgaacgtg atcttcgact tgcacgacgc cgtgttcagg aacgcggggt
     9721 cgtacgtcat caggccgaag tcctccgggt tcaccttgat cttccggagg tccatcgcct
     9781 tgat
//
LOCUS       DAOONL010000126         9778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8041_length_9778_cov_10.996400, whole genome shotgun sequence.
ACCESSION   DAOONL010000126 DAOONL010000000
VERSION     DAOONL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_9778_cov_10.996400"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLP50_14210"
     CDS             <1..218
                     /locus_tag="PLP50_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52745.1"
                     /translation="DYLVSEERADKLSGAWGRHPLLGRMYLPAYRAGTEKVAELRRKY
                     PREKVLPALFGCRGVVDLTTIEEAVTA"
     gene            complement(247..1608)
                     /locus_tag="PLP50_14215"
     CDS             complement(247..1608)
                     /locus_tag="PLP50_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPA52746.1"
                     /translation="MNGYRPRRSCAGAVLLLLFPLFSGACSGAPGSRPSAAPPPLAEA
                     RPAGERLGLEEAGQGLPRSGQWRHSFALADVDGDGRLDLVHGPARKGVRRPVVFLGNG
                     EGGFRRWDEAQFPPFPYDYGGVATGDVDGDGRLDLALGIHLRGLAVLLQKQPGVFSLF
                     VDGLPVGQAGKPADFSSRAVALFDADGDGRLDLLALSEGPLSPTALAAPGARSPLGLA
                     LFLNSGGAWERTLDGPAADRTFGSSLAVGDVDGDGALDVLLGSGAQGDRALLRLSRRA
                     GDPRFLPVELTEIPERSFVQAVALGNLDGDGRPDLLVSSLASGAGGFSSRVDVLFSRP
                     PGFERRPVLSEAGRKPRGAVAIGDLDGDGRPDLAVLSGDGTLETYLGDGRGGFSAAAR
                     VAPPPERVGCAGYHVAIRDLDGDGRGDVVAAFAGEGSASDPEAPCRSGGALVAWRSVA
                     PRR"
     gene            complement(1608..2648)
                     /locus_tag="PLP50_14220"
     CDS             complement(1608..2648)
                     /locus_tag="PLP50_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HPA52747.1"
                     /translation="MRSLLASRLLPRTPLVHAALVGAVLVVFPANAQLANCTALAGTN
                     CREVIPDGGILRGVSFSGTLASTLTVPDGTCGAFTNVVDVNLQVRLKHNWVGDLRLRL
                     VHPNGTTAAFALQNPGGPPAGISGEDLDATFNDEGPSLSISSAIPTISGTVQPVGSFS
                     VFDGLPRAGTWTLEVADSRGGNYGALEDWTLELPCSLPTVSIAATVPDAVEQPVTSGQ
                     FTITRTGGDTSVPLFVPFTISGTATQGVDYAPLSGALTLPAGVTTAAISVLPIVDAVF
                     DNGEGVTMTLGPGDYTIGTGIATVWIWENPTRVGQLEQSIPTSSPVGLSLLALAVAAV
                     GAGLLGSRKLLG"
     gene            complement(2663..3910)
                     /locus_tag="PLP50_14225"
     CDS             complement(2663..3910)
                     /locus_tag="PLP50_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52748.1"
                     /translation="MKKSLLLAALLALLVVPSAPAFAAIGANGPTPAATLLLPYFEVD
                     LANPGGVNTLFTVANASASAGVVHVTLWTELGIPTYAFDIYLTGFDAETVDLRLLFQG
                     IAPISADDGSDPTDRISPQGIISQDINFPGAVPPCANGPIPRMSPAQVLDLQRAHTGL
                     SSSIRAGWNATCGARPHGDDIARGFVTIDDVVICSDAVPTDPGYFTDIIGRRNIWFGE
                     FLILDRENNFAIGDALAHVEASYSDPRVTDPAAHTFYGWVRGYAGTSDSREPLPTAWR
                     TRYVDAGAFDGGTDLLVWRAPPGPVGTFACGGALPAPFPLPQRYLAVFDEQENPFVPA
                     AGSYFPVAAERVPVSSLSPPSPFGFLHLDLKRGSDPSPGALAGVGQSWISTDLRATGR
                     FSVGFGARALDNALDPFLAILGN"
     gene            complement(3907..5223)
                     /locus_tag="PLP50_14230"
     CDS             complement(3907..5223)
                     /locus_tag="PLP50_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52749.1"
                     /translation="MTKPWVLPLVLALSLGVAAPAEGVIGVPDPVPAATLLYPYFEVD
                     LANPNGVTTLLGLQNTSATAVLAHYVVWTDLGVPVLNFNVYLTGYDVQTINLRDVIAL
                     GMLPTTASAGQDPADTISNQGIFSQDINFASCTGQLPLPPLSADVVAGVRAALTGQAS
                     TTFGGLCGGTNRGDSIARGYVTVDTVNSCTLVYPGNPGYFGPGGSGVATNQNVLLGDF
                     FLIDPAQNYAQGEGAVSIEASATDPLTSTSGNYTFYGRYVGWTANDNREPLPTIWGAT
                     YTDTASELFVWRDPKVSQAPFTCPVTPAARPAWYPLGQTQIVAFDMQENPTALGGTPF
                     AAAAQRVTLGMTGGDLVTPARSGWLFLNLNTSVAAAGAVPPSDPVRAQSWVVSLQLPK
                     AEGRFSTATAAVPLDSDAYRTGSIPLLSKPGAPTASPASKAGRSGK"
     gene            complement(5362..5913)
                     /locus_tag="PLP50_14235"
     CDS             complement(5362..5913)
                     /locus_tag="PLP50_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52750.1"
                     /translation="MTRILRLTAGLVLLAALVLDFRRHAGERSLYRATALLGSALAGS
                     APGTDPRRLAEAARDLAAEASAALPYDPRPPLVEGASRLALGDSARAEACFRESVERA
                     EKPEALVNLGRALLVRGRHEEARHVFVRAAWLHPVSLATLPTSFREPALERAEAGRAR
                     LRSDGELSVPSVPPVTDPGAEPR"
     gene            complement(5910..7250)
                     /locus_tag="PLP50_14240"
     CDS             complement(5910..7250)
                     /locus_tag="PLP50_14240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPA52751.1"
                     /translation="MTKRAKRNEPMPAVGPAARATRDLSGGLAAVTVSAALLGILLAV
                     DPRAESSFDSIKTTVAHAGAVLATVLLLVGGAGPARSLRSLPRPVPALFALAAAGSLL
                     AVLSAAFAPRPAAAFEALRSASLLALLVPVGASRALEGGRVVLPVGAFLAGLSVNAAA
                     SLLQAAGALTLFDVSRISGRSNTGAFVGNEGYLSLVAALAIPVAAGAVAAARGRARLG
                     AGAALALALAAVLANRSLTGLAAMAAGLLVFLWAGLSGRTRRITIAGCFILAATLPFV
                     PSVRLRLAEVVVQVRTGNADALISYRGGPWAAAAEMIRERPVLGYGPGAFESRFVPSR
                     LAAERRLGRPLTTLTEAGSYAEAHSDVLQLPAEIGLPAAGAFAASLALLLAALLRRAR
                     RAEGVEAAVLLAVLATGLVAALTWFPLQRAVTAAPLLLAVGRTLRLCSRERTEP"
     gene            complement(7275..>9778)
                     /locus_tag="PLP50_14245"
     CDS             complement(7275..>9778)
                     /locus_tag="PLP50_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52752.1"
                     /translation="SRGRLAFFAACLGTGVAAIVAVAGLSGALEETIRSQARPLLGAD
                     LSIESFRPFPESVDQAIEALPGVRTRVEELVTVVFEPGTAAANEGAAPRSLLVELKAV
                     EPPYPLVGELRLEPDRPLADLLAPDAIVAHPDLLRRLGVAVGEDVSLGGERFRVAGTI
                     AAEADRLAGSFRIGPRVFVSREGLARTKLAGFGSRVTRRVLLNFPEGTDAAVARKIVT
                     GLKDRLESVPGIRVESWLDGQPELRQAIRRTSRFLGLVALTSLLVGGVGVAQTVRAWI
                     ASRLDALAILRCLGVTSREAILLYAVQTAGLGLLGSLLGAVAGIGLVAAVGQAYSDLL
                     PPGGLDPWQPAALLRGLGLGLGVSLLFGVPALLAVRRVPPARVLRRDAEPIPASLPLR
                     ILGGVVLFAGVGLTAWVQSGLPRVATLFTLGLAASALVLALSARGLLWLVTHVPRERL
                     TFALRHGLAALVRPGAGTVSSILALGLGTLVVLHIALVQRDFSRQLAADLPSNAPTAF
                     LLDVQPDQWVGVSAVLSEQGATRVDSVPVVTARLTAVDGTDTSDLAARMKGDARRQRW
                     ALTREQRLTYMETLPADNVIVAGALWSDPGALEVSLEEEFAGRIGARLGSVLDFDVQG
                     VPLRLKVTSLRTVDWRTFGINFFLVVEPGVLEQAPQLRLAAARLPKESEGRIQDLLAR
                     EFPNVILIRTREVLERVAELASRMGEAVRVLGAFSVLAGLVILGGSISAASARRSREV
                     ALLKVLGVTRAGAALALAIEYALVGLVAGLVGTLGATLLSGAVLKAAFEVSFQPSAAL
                     LTLAPLGVSLLAAAAGLAVSVRALRVRPLEVLRGE"
BASE COUNT         1511 a         3146 c         4000 g         1121 t
ORIGIN      
        1 acgactacct cgtctcggag gagcgggccg acaagctctc gggcgcctgg gggcggcacc
       61 ctctcctcgg ccggatgtac ctgccggcct accgggccgg gaccgagaag gtggccgagc
      121 ttcggcggaa gtacccgcgc gagaaggtcc tcccggcgct cttcggctgc cggggcgtcg
      181 tcgacctgac gacgatcgag gaggccgtga cggcctgacc ccggggatgg cgggggtcgg
      241 tgctcctcac cggcgagggg cgaccgacct ccaggcaacg agcgcgccgc ccgaacggca
      301 cggggcctcg gggtccgaag cggagccttc gccggcaaac gcggcgacga cgtcgccccg
      361 gccgtcgccg tcgagatccc ggatcgcgac gtggtatccc gcacagccga cgcgttcggg
      421 agggggtgcg acgcgtgcgg cggcggagaa gccgcccctt ccgtccccca ggtacgtctc
      481 gagcgtgccg tccccggaaa gaacggcgag gtcgggccgg ccgtcgccgt cgaggtcgcc
      541 gatggcgacg gccccccggg gcttccggcc cgcctccgac aggaccggtc tcctctcgaa
      601 ccccggcggg cgggagaaga gaacgtcgac gcgggacgag aatccgccgg ccccggaggc
      661 gagggaggag acgaggaggt cgggccggcc gtcgccgtcg aggttgccga gcgccacggc
      721 ctgcacgaac gaacgctccg ggatctccgt cagctcgacg gggaggaagc gggggtcgcc
      781 cgcgcggcgt gacaggcgga ggagcgcgcg gtcgccctgc gcacccgagc cgaggaggac
      841 gtcgagggcg ccgtcgccgt ccacgtcgcc gaccgcgagg ctgctgccga aggtgcggtc
      901 agccgcgggg ccgtccaggg tccgctccca ggcgccgcca gaattgagga agagggcgag
      961 gccgagaggg gagcgcgccc cgggggccgc aagggccgtc ggcgagagcg ggccttcgga
     1021 gagggcgagc aggtcgaggc ggccgtctcc gtccgcgtcg aagagggcga cggcccgcga
     1081 ggagaaatcg gccggctttc cggcctggcc gaccggcagt ccgtcgacga agagggaaaa
     1141 gacgccgggc tgcttctgga gcaggaccgc gaggccgcgc aggtggatcc cgagggcgag
     1201 gtcgagccgg ccgtcgccgt cgacgtctcc cgtggcgacg ccgccgtagt cgtaggggaa
     1261 gggagggaac tgcgcttcgt cccagcgccg gaagccgccc tcgccgttgc cgagaaagac
     1321 gacgggacgc cggaccccct tgcgcgcggg gccgtggacg aggtcgaggc gcccgtcgcc
     1381 gtcgacgtcg gcgagtgcga acgagtggcg ccactggccc gaccggggaa gcccctgtcc
     1441 ggcttcctcg agcccgagtc tctccccggc cgggcgggct tcggcgagcg gcggcggggc
     1501 ggcggagggc cgcgagccgg gagcccccga acaggccccg gagaagaggg gaaagagaag
     1561 gaggagaacg gccccggcgc aggaacgccg gggccggtac ccgttcatca gccgaggagc
     1621 tttctggacc cgaggagccc ggcgccgacc gccgcgacgg ccagcgcgag gagagacagg
     1681 ccgacggggg acgaggtcgg gatcgactgt tcgagctgcc ccacgcgcgt cgggttttcc
     1741 cagatccaga cggtcgcgat gccggtcccg atcgtgtagt cgcccggacc gagagtcatg
     1801 gtcacccctt cgccgttgtc gaagacggca tccacgatgg gcaggacgga gatcgcggcc
     1861 gtcgtaacgc cggcgggcag ggttagggcg ccggagaggg gggcgtagtc gacgccctgg
     1921 gtggcggttc cggagatcgt gaaggggacg aagagcggga ccgacgtgtc cccacccgtc
     1981 cgcgtgatcg tgaactggcc gctcgtcacg ggctgttcga cggcgtccgg cacggtggcg
     2041 gcgatcgaga ccgtcggcag cgagcacgga agctcgaggg tccagtcctc gagggcgccg
     2101 tagttgccgc cccgggagtc ggcgacctcg agggtccagg tgccggcgcg gggcaggccg
     2161 tcgaagaccg agaagctccc cacgggttgg accgtccccg agatggtcgg gatggcgctc
     2221 gagatggaga gggaaggccc ctcgtcgttg aacgtcgcgt cgaggtcctc gccggagatg
     2281 ccggccggag ggcctcccgg gttctgcagg gcgaaggcgg ccgtggtgcc gttcgggtgg
     2341 acgagacgga gccggaggtc cccgacccag ttgtgcttca ggcgcacctg gaggttgacg
     2401 tcgacgacgt tcgtgaaggc gccgcaggtc ccgtcgggga cggtcagggt ggacgcgagg
     2461 gttccggaga agctcacacc ccgaaggatt ccgccatccg ggatgacctc gcggcagttc
     2521 gtgcccgcga gggcggtgca gttcgcgagc tgggcgttcg cggggaagac gacgaggacg
     2581 gcgccgacga gggcggcgtg gacgaggggc gtgcgcggga ggagacggga ggcgaggagg
     2641 cttctcatgg tcgtcgtcct cctcagtttc cgagtatggc gaggaagggg tccagcgcgt
     2701 tgtcgagggc tctcgccccg aagccgaccg agaaccggcc ggtcgcgcgg aggtccgtgg
     2761 aaatccagga ctggcccacg ccggcgagcg cgccggggga ggggtcgctt ccacgcttca
     2821 ggtcgaggtg gaggaagccg aaggggctcg gcggggatag ggaggagacg gggacccgct
     2881 ccgccgcgac cgggaagtag gagccggccg cgggcacgaa gggattctcc tgctcgtcga
     2941 agacggcgag gtacctctgg ggaaggggga agggggcggg aagggctccg ccgcaggcga
     3001 aggtgccgac cggcccggga ggcgcgcgcc agacgaggag gtccgtgccg ccgtcgaagg
     3061 cgccggcgtc gacgtatcgc gttcgccagg ccgtcggaag cggttcgcgg gagtcggagg
     3121 tgccggcgta gccgcggacc cagccataga aggtgtgggc ggcggggtcg gtcacgcgcg
     3181 ggtccgagta gctcgcctcg acgtgggcga gggcgtcccc gatggcgaag ttgttctccc
     3241 ggtccaggat caggaactcg ccgaaccaga tgttcctgcg cccgatgatg tcggtgaaat
     3301 agccggggtc cgtcgggacc gcgtcggagc atatgacgac gtcgtcgatc gtgacgaacc
     3361 cgcgggcgat gtcgtcgccg tgggggcggg cgccgcaggt ggcgttccag ccggcgcgga
     3421 tgctcgacga gagccccgtg tgggcgcgct ggaggtccag gacctgcgcg gggctcatcc
     3481 ggggaatcgg cccgttggcg cagggcggga ccgcgcccgg gaagtttatg tcctgcgaga
     3541 tgatcccctg cgggctgatg cggtcggtcg ggtccgaccc gtcgtcggcg gagatcggcg
     3601 cgatgccctg gaagaggagc cgcaggtcga cggtctcggc gtcgaagccg gtgaggtaga
     3661 tgtcgaatgc gtacgtcggg attccgagct cggtccagag cgtcacgtgg acgaccccgg
     3721 cgctcgccga ggcgttcgcg acggtgaaga gcgtgttcac gccgccgggg ttcgccaggt
     3781 cgacctcgaa gtaggggagg aggagggtgg cggccggcgt cggcccgttg gcgccgatgg
     3841 cggcgaaggc gggagcggag gggacgacga gcagcgcgag gagcgccgcg agcaggaggg
     3901 acttcttcac tttccggacc tcccggcctt cgaagcgggc gaggccgtcg gcgcccccgg
     3961 cttcgagagc agagggatcg agccggtgcg gtaggcgtcg gaatccagcg ggaccgctgc
     4021 cgtggccgtc gagaagcggc cctcggcctt gggcagctgg agcgagacga cccaggactg
     4081 cgccctcacc gggtcggatg gcggcacggc tcccgcggcc gcgaccgagg tgttcaggtt
     4141 caggaagagc cagccggagc gggcgggcgt gacgaggtca ccgccggtca ttccgagcgt
     4201 cacccgctgc gcggccgcgg cgaacggcgt tccgccgagc gcggtggggt tctcctgcat
     4261 gtcgaaggcg acgatctggg tctgaccgag gggataccag gccgggcggg cggccggggt
     4321 gaccgggcag gtgaacggag cctggctcac cttcgggtcg cgccagacga agagctcgga
     4381 ggcggtgtcg gtgtaggtcg ccccccagat cgtcgggagc ggctcccggt tgtcgttggc
     4441 cgtccacccg acgtaccggc cgtagaaggt gtagttcccg gacgtcgacg tgagcgggtc
     4501 ggtcgcgctc gcctcgatcg agacggcgcc ctctccctgg gcgtagttct gcgcggggtc
     4561 gatgaggaag aagtccccga ggaggacgtt ctggttcgtg gcgactccgg agcctccggg
     4621 cccgaagtaa cctgggttgc ccgggtagac cagcgtgcag ctgttcacgg tgtcgaccgt
     4681 gacgtagccg cgggcgatgg aatcgccgcg gttcgttccg ccgcagaggc caccgaaggt
     4741 cgtggaggcc tggccggtca gcgcggcccg gaccccggcg acgacgtcgg ccgagagcgg
     4801 gggcagcggg agctgcccgg tacaggaggc gaagttgatg tcctgagaga agatcccctg
     4861 gttactgatc gtgtcggccg ggtcctgccc ggccgatgcc gtcgtgggca gcattccgag
     4921 agcgatcacg tcgcgcaggt tgatcgtctg gacgtcgtag ccggtcaggt agacgttgaa
     4981 gttcaggacc ggcacgccga gatcggtcca tacgacgtag tgggcgagta ctgccgttgc
     5041 ggacgtgttc tggagcccga ggagcgtcgt caccccgttg ggattggcca ggtccacctc
     5101 gaagtagggg tagaggaggg tcgccgccgg cacggggtcg ggcaccccga tgacgccctc
     5161 cgcgggagcg gcgacgccca gagacagggc gaggacgagg ggaaggaccc atggtttcgt
     5221 cattcggact cctcgggccg gtcgagaggg cacggtctgg ttctctccag acggtgtccg
     5281 gaaagagaag ggcatcgaga gatcgcgatg ccgacggttt caaggcggtc ccgttattag
     5341 acgcgacctc aaatgatccc gtcaacgggg ctcggccccc gggtccgtga cggggggcac
     5401 ggaaggtacg gacagctcgc cgtccgagcg gagcctcgct cttccggcct cggccctctc
     5461 gagggcgggt tcccggaacg aggtcgggag cgtcgcgagg gacaccgggt ggagccacgc
     5521 cgcgcgaacg aagacgtgcc gggcctcctc gtgtcgtccc cggacgagga gcgcccggcc
     5581 gagattcacg agggcctccg gtttctccgc acgctcgacg gactcgcgga agcaggcctc
     5641 cgcccgcgcg gagtcgccga gcgcgagccg cgacgctccc tcgacgagcg ggggtcgggg
     5701 gtcatagggg agagcggcag aggcttcggc cgcaaggtcg cgggcggctt cggccagacg
     5761 gcggggatcg gtccccggag cgctgcccgc gagcgcgctc ccgaggaggg ccgtggcccg
     5821 ataaagggag cgctctccgg cgtggcggcg gaaatcgagg acgagggctg cgagcaagac
     5881 gaggccggcc gtgagccgga gaatccgcgt cacggttccg tcctctctcg ggagcagagg
     5941 cggagagtgc gcccgacggc gagaagcagc ggcgcggcgg tcaccgccct ctgcagggga
     6001 aaccaggtca gggccgcgac gaggccggtc gccagaaccg cgagaagaac cgccgcttcc
     6061 actccctctg cccgtcgggc gcgtcggagc agggcggcga gaaggagcgc gagcgaagcc
     6121 gcgaaggccc cggccgcggg aagtccgatc tcggccggca gctgcaggac gtcggagtgc
     6181 gcctccgcgt acgagccggc ctccgtcagg gtcgtgaggg ggcgtcccag gcgccgttcc
     6241 gcggcgaggc gcgatggaac gaagcgggac tcgaaggctc ccgggccgta gccgaggacg
     6301 gggcgttcgc gaatcatctc cgccgccgcc gcccagggac cgccgcggta gctgatgagc
     6361 gcgtcggcgt tcccggtccg gacctggacg acgacctccg cgaggcggag acggacggac
     6421 ggcacgaacg gcagcgtcgc ggccagaatg aagcagccag cgatcgtaat ccgccgcgtt
     6481 ctccccgaga ggcccgccca gagaaacacg agcaggccgg cggccatggc ggcgaggccc
     6541 gtgaggctcc ggttcgcgag cacggccgcg agcgcgagag cgagagcggc cccggctccg
     6601 agccgggcgc ggcctcgggc cgcggcgacg gcgcccgcgg cgacggggat ggccagagct
     6661 gcgacgaggg agaggtagcc ctcgttgccg acgaaggccc ccgtgttcga gcgtccggag
     6721 atccgcgaga cgtcgaagag ggtgagagcc ccggccgcct gcaggagcga cgcggcggcg
     6781 ttgacggaga gtcccgcgag aaacgcaccg acgggaagga ccacccttcc gccttcgagc
     6841 gcccgggagg ccccgacggg gacgaggagg gcgaggagcg aggcggagcg cagggcctcg
     6901 aaggcggccg ccggacgcgg cgcgaaggcc gcggagagaa cggcgaggag agacccggcg
     6961 gcggcgaggg cgaagagcgc gggaaccggg cgagggaggg aacggagaga gcgggccggt
     7021 ccggctccgc ccacgaggag gaggacggtc gccaggacgg ccccggcgtg cgcgacggtc
     7081 gtcttgatcg agtcgaagga cgactctgct cgcgggtcga ccgccagcag gatcccgagg
     7141 agcgcagccg agacggtgac ggcggcaagg ccgccggaga ggtcccgcgt cgcgcgggcc
     7201 gcaggcccca ccgccggcat cggctcgttt cgcttcgcgc gtttcgtcaa ggggcgggga
     7261 ttatgccctc ccgcctactc gccccggagc acctcgagcg gccggacacg gagcgctcgc
     7321 acgctcacgg cgagccccgc ggcggcggcg agaagggaga cgccgagggg cgcgagggtg
     7381 aggagcgctg cggagggctg gaaggagacc tcgaaggcgg ccttcaggac ggcgccggag
     7441 aggagggtgg cgccgagggt cccgacgagc cccgcgacga gaccgacgag ggcgtactcg
     7501 atggcgaggg cgagcgctgc gccggcgcgc gtgacgccga ggaccttgag gagcgcgacc
     7561 tcgcgggacc ggcgggccga cgccgcgctg atggagcctc cgaggatcac gaggcccgcc
     7621 aggacggaga acgccccgag gacgcgcacc gcctcgccca tccgcgaggc gagctcggcg
     7681 acgcgctcga ggacctcgcg cgtccggatg aggatgacgt tcgggaactc ccgcgcgagg
     7741 aggtcctgga tcctcccctc gctctccttc gggaggcgcg cggccgcgag gcgcagctgg
     7801 ggcgcctgct cgaggacgcc cggctcgacg acgaggaaga agttgatgcc gaaggtccgc
     7861 cagtcgaccg tccggaggct cgtcaccttc agccggagcg ggacgccctg gacgtcgaag
     7921 tcgaggacgg agccgaggcg cgcgccgatc ctccccgcga actcctcctc gaggctcacc
     7981 tcgagagcgc ccgggtcgga ccagagggcg ccggcgacga tcacgttgtc ggcagggagg
     8041 gtctccatgt aggtgagccg ctgctcgcgc gtgagggccc agcgctggcg acgggcgtct
     8101 cccttcatcc gcgcggcgag gtcggacgtg tcggtgccgt cgacggccgt gagacgcgcg
     8161 gtgacgaccg ggaccgagtc gacgcgcgtc gctccctgct ccgagaggac cgccgaaacc
     8221 ccgacccact ggtcgggctg gacgtcgagg agaaacgcgg tcggcgcgtt cgaagggagg
     8281 tcggccgcga gctgccgcga gaagtcgcgc tggacgaggg cgatgtggag gacgacgagc
     8341 gtgccgagcc cgagggcgag gatcgagctg acggttcccg ccccgggccg tacgagcgcg
     8401 gcgaggccgt gtcggagcgc gaacgtcagc cgctcccgcg ggacgtgcgt cacgagccag
     8461 aggagtccgc gggcggagag ggcgaggacg agcgccgacg cggccagccc gagcgtgaag
     8521 agggtcgcca cgcgcggcag gcccgactgg acccaggcgg tcagtccgac gccggcgaag
     8581 aggacgacgc cgccgaggat gcggagcggg agggacgccg ggatcggctc ggcgtcgcgg
     8641 cgcaggacgc gcgcgggcgg cacgcgccgg acggcgagga gggccgggac gccgaagagg
     8701 agggagacgc cgaggcccag gcccaggccg cgcaggaggg cggcgggctg ccacgggtcg
     8761 aggccgcccg gcgggaggag gtcggagtag gcctggccga cggctgcgac gaggccgatc
     8821 ccggcgacgg cgccgaggag gctcccgagg aggccgaggc ccgcggtctg gacggcgtaa
     8881 aggaggatcg cctcgcgcga ggtgacgccg aggcagcgga ggatcgcgag ggcgtcgagg
     8941 cggctcgcga tccaggcgcg gaccgtctgg gcgacgccga cgccgccgac gaggagcgag
     9001 gtgagggcga cgaggccgag gaagcgggag gtgcgccgga tggcttgcct cagctccggc
     9061 tggccgtcga gccagctctc gacccggatt cccggaacgc tctcgagtcg gtccttcagc
     9121 cccgtgacga tcttccgcgc gacggcggcg tcggtcccct cggggaagtt caggaggacg
     9181 cgccgggtga cgcggctgcc gaaaccggcg agcttcgtcc gcgcgagccc ctcgcgcgag
     9241 acgaagaccc gcgggccgat gcggaaggag ccggcgaggc ggtcggcctc ggccgcgatg
     9301 gtgccggcga cgcggaagcg ctcgccgccg agggagacgt cttccccgac ggcgacgccg
     9361 aggcggcgga ggaggtccgg gtgggcgacg atcgcgtcgg gcgcgaggag atcggcgagg
     9421 ggccggtcgg gctcgaggcg gagctcgccg acgagagggt agggcggttc cacggccttc
     9481 agctcgacga ggaggctgcg cggggcggcc ccctcgttcg ccgcggccgt ccccggctcg
     9541 aagacgaccg tgacgagctc ctcgacccgc gtccggacgc cggggagggc ctcgatcgcc
     9601 tggtcgaccg actcgggaaa cggccggaag gactcgatcg agaggtcggc gccgaggagg
     9661 gggcgggcct ggctccggat cgtctcctcg agcgccccgg agagccccgc gaccgcgacg
     9721 atcgccgcga cgccggttcc gaggcaggcg gcgaagaacg cgaggcgccc gcgcgacc
//
LOCUS       DAOONL010000127         9727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8098_length_9727_cov_19.516129, whole genome shotgun sequence.
ACCESSION   DAOONL010000127 DAOONL010000000
VERSION     DAOONL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_9727_cov_19.516129"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PLP50_14250"
     CDS             complement(<1..1342)
                     /locus_tag="PLP50_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA52753.1"
                     /translation="MIAGILGAIRPHPCGTISCRVPRGTSEADRQESRIRTLVPSRLR
                     LLAGSAVLACAVASGACTSAPPAAQAAPASPAPQAAVRPAAPPLLDDWRDAVLYFVVL
                     DRFADGDAASNVDVDASKKGHFHGGDAKGLTAQLDEIASLGVNALWITPVVTNTPGFV
                     TGAGFPDWAYHGYWADDFLSLDPRFGSENDFIALVEACHARGIRVLLDVVYNHAGYES
                     RYKKDPRTKGWFRTNEKGDCGADDVTSCVAGLPDFRTEDPEIAKYLLDAQIAWAKRLG
                     VDGFRLDTVKHVAHDFWTEHRRRTREEVGKGFFLLGEVWGGDAQVLDPWFERDEMDAG
                     FDFAFQGNALAFVEGRGRTVAFDRYLKSREKVRPGYLLSHFLSSHDVPGGLFQLGGNV
                     ELFRLAAVLQMTTAGIPTIYYGEEVARPGGDWPENRSDMPWGARDVRPGAGLPRN"
     gene            1350..2630
                     /locus_tag="PLP50_14255"
     CDS             1350..2630
                     /locus_tag="PLP50_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPA52754.1"
                     /translation="MPNRRVHTVILGGGQGKRLFPLTRDRAKPAVPLGGKYRLIDVPL
                     SNAINSGFRDIAVLTQFNSASLNNHISLTYRFDSFARGQVELIAAQQTDEGGEWFEGT
                     ADAVRKHLRRLGTRDYDHVLILAGDHLYRMDYREMFDAHVQSSADVTVGVLPVGVEEC
                     SGFGILLTGSDGQIRAFREKPKTEEELAPLAPAKELRDRWGLQPGQYLASMGVYVFRM
                     ETLREALAKPANMDFGKDIIPGHIGTHKVQAFPFRGYWRDIGTIRSFYEANIALTDDD
                     PPFHFHHPNAPIFTRPRFLPSSSLIDVKVDHCRLADGVRIYGAEVTRSVIGLRARIEK
                     GGRIVESILMGADEFEDEDLAVSALNEGLPPVGIGADCVIERAIVDKNARIGAGCVLR
                     GDPSRPDQDGDGWCVRDGIVIVPKDAVLRPGTVV"
     gene            2695..4299
                     /locus_tag="PLP50_14260"
     CDS             2695..4299
                     /locus_tag="PLP50_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPA52755.1"
                     /translation="MPPVSFVLVLHGHMPAVLGHGVWPHGADWLHEAAAETYLPFVAA
                     ARELDAEGVPWRATIGLTPILCEQLRDPRFPRSFRRYVTHRLRAAGRDAREFARDGHV
                     AEPLAHAWRAHYEEGLALFDAWGGDLVAPFVRLAEAGRIEAIASAATHGFLPLLPSDA
                     AAERQVDVGLAAHRRHFGRAAHGFWLPECATAPAGTLVTPGHGRRVARRSVPSLLASR
                     GLAFTFVDTHLAAGPAAGTAAGGSAGRRWPAAGGSPAARSPYRVHRVRTDGGSLSLLA
                     RDPRSSEQVWSGEVGYPGDPAYLEFHRKAARGGLRYWSVTDARGPLDAKELYEPEETR
                     VRVATHASHFCDLLEETARMESDARVVVAMFDFELFGHWWFEGVAFLAEVFRELARRN
                     GPVRAATAGEAVREAGADAPEIEPGPGSWGRGGDFRVWDDAKTKPFWRSVEEAEAQLR
                     RIEARDPRLLPTALRQALLLQASDWPFLVEAGTARDYAQRRIEGHAGALRALAELAGR
                     PRSRVDALFLARLARFDRAFRPELGG"
     gene            4386..4988
                     /locus_tag="PLP50_14265"
     CDS             4386..4988
                     /locus_tag="PLP50_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HPA52756.1"
                     /translation="MAYTAKSYDHLLGTPGFSDALMKNHFTLYQGYVTNTNKVNDELA
                     ALAADGKAATPAFAEMKRRLGWEWNGMRLHEYYFGNMKNGGAALDGGSALAKKLAADF
                     GSVEAWEKNFRAVGAMRGIGWTALYQDPVSGQLMNMWIGEHDGGHPAGGTLLMIMDVF
                     EHAFMLDYGLKRPDYIEAFFKAIDWSVVSARFDAAPKLAL"
     gene            complement(5107..9564)
                     /locus_tag="PLP50_14270"
     CDS             complement(5107..9564)
                     /locus_tag="PLP50_14270"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA52757.1"
                     /translation="MTARRGSGPAADPLDLFHPAVAAWFLETFPEGPTEAQRLAWPAI
                     ARGESVLLVSPTGTGKTLAAFLVALDGILRRRLAGDDAPRLATLYVSPLKALDNDVHR
                     NLERPRAGIAAQLGAGRVPITTAVRTGDTPTRERTSQLKAPPDVLITTPESLYLMLTG
                     GGRRALEGVSTVVLDEVHSVAGTKRGSHLALSLERLEEVIARAGGAPPQRVALSATVA
                     PVDVVASFAAGTGRKTKPLVVPAKKALDLVVASPAPDFRDLPDGSAWGPAVEAVVREV
                     ESRTTTLVFCNNRRLAEKVSRKLSDATGADVPTHHGSVSRPRREEIEKELKAGTLPVL
                     VATGSLELGIDVGHVDSVVLLESPKGIARGLQRVGRSGHLVGETARGLFVPLYLDDLF
                     ESAATAEAMREGAVEETRPPDFPLDVLAQQLVAEAVARAADGLAVTAAELFALARKAY
                     PYRALPRSLFLETLAMLSGKYPRERFRELAPKLVWDRATDRVTPLPGARIAALLDGGT
                     IGDRGAFRAVLADRKTLVGELDEEFVHETKEGDVFLLGSRSWRAVEIGPSAVVVEPAP
                     GQPTPRMPFWRGEGLGRTGDLGQRIGRLRRFVAERLDDPGLNEEVSRRYATTPDATAA
                     IVGAVRREATESGGLASDLAVVFETFPNDLGDPCIVVRSLFGRGVNLPWSLVLSAVLR
                     EETGVDVETVASDDGILLRIPGAEREVPLERLSRISAAEAKERLLAQLPNSPMFGARF
                     RENAQRALLLPRNRSGRRTPFWLQRIKAKDLLQTTRSLPDFPVVAETYRDCLRDLWEM
                     DRLLGLLDRMEKGTVARLLEKRRAPSPAAASLLFKFVAVYMYEWDAPRAERDLHAVAA
                     NRALLGEVLGESLDDGLRPEAAEAARADATRLSPHRMARTAEELLLALMENQDLTAPE
                     AIERCAGDGAAWVAELTARGSIVRMRVGGEERLVPAEERARFEALASGTLSREERDRL
                     VLGYVGTRGPVTAAEVAARYGLAEDDVLDSLERLRLEALVVAGRLGGPPGERRFAGAR
                     LAEAIRRRTLGILRGEIRPVPRSVLRAFVARRQGAVAGSRFAGPDAAERAVGLLRGLA
                     LPVASWEASALPARLAEPEPDALDVLAARGLLVFRLAGEKELKAARMSLFFRGEGRFV
                     LPEAPAGLDALSADARALLEALGLGGASFLSDLGGALGKRPEALVAPLRELLLAGLVS
                     GDGFHGLRELLRAKRIAPAPAAPPARLGAVPSRRELRAAEARVATRLGFTPRGPATAS
                     HVLSGRWALLGAPSVLGPELAPGERSEAWARLLLARWGVVSRGVVEEAESAVRWSDVA
                     PAFARMELRGDVRRGEFVEGSGPLQYAEPDVVEELRRMREARDEEDPRAELAALAGVD
                     PVLVGVEVPRDGWCVLSRGVPVATLSGAGALDLGGEPAPDRVLRAALAELQELLRRGR
                     DPLGRPRRLVVATVASGAAPRPAAGSAVAPLLEAAAFTRDGTGYAWRAL"
BASE COUNT         1312 a         3523 c         3692 g         1200 t
ORIGIN      
        1 cgttccgcgg gaggccggcg ccgggcctca cgtcccgcgc gccccacggc atgtcgctcc
       61 ggttctcggg ccagtcgccg ccggggcggg ccacctcctc gccgtagtag atcgtcggga
      121 tgccggccgt cgtcatctgg aggaccgccg cgagccggaa gagctccacg ttcccgccca
      181 gctggaagag gccgcccggg acgtcgtgcg aggagaggaa gtgggagagg aggtagccgg
      241 ggcggacctt ctcgcgcgac ttcaggtagc ggtcgaacgc gaccgtccgc ccgcgtccct
      301 cgacgaacgc gagggcgttc ccctggaacg cgaagtcgaa gccggcgtcc atctcgtcgc
      361 gttcgaacca cgggtcgagg acctgcgcgt cgccccccca gacctcgccg aggaggaaga
      421 acccctttcc gacctcctcg cgcgtccgcc gccggtgctc ggtccagaag tcgtgcgcga
      481 cgtgcttgac ggtgtcgagg cggaagccgt cgacgccgag ccgcttcgcc caggcgatct
      541 gggcgtcgag gaggtacttc gcgatctcgg ggtcctcggt ccggaagtcg gggaggccgg
      601 cgacgcacga ggtgacgtcg tccgcgccgc agtccccctt ctcgttcgtc cggaaccagc
      661 ccttcgtccg cgggtccttc ttgtagcgcg actcgtaccc ggcgtggttg tagacgacgt
      721 cgaggaggac gcggatgccg cgcgcgtggc acgcctccac gagcgcgatg aagtcgtttt
      781 cagagccgaa gcgcgggtcg agggagagga agtcgtcggc ccagtagccg tggtaggccc
      841 agtcggggaa gccggccccg gtgacgaagc cgggggtgtt cgtcacgacc ggcgtgatcc
      901 agagggcgtt cacgccgagc gaggcgatct cgtcgagctg agccgtcagc cccttcgcgt
      961 cgccgccgtg gaagtgcccc ttcttcgagg cgtcgacgtc gacgttgctc gccgcgtcgc
     1021 cgtccgcgaa gcggtcgagg acgacgaagt agaggacggc gtcgcgccag tcgtcgagga
     1081 gcggcggcgc cgccggccgg acggcggcct gcggggccgg cgaagccggg gcggcctgcg
     1141 ccgccggagg ggccgacgta catgcgcccg atgcaaccgc gcaggcgaga acggcgctcc
     1201 ccgcgaggag acggaggcgg gacggaacga gcgtgcgaat acggctctcc tgtcggtcgg
     1261 cttccgatgt tcctctcgga actctacagg aaatcgtccc gcagggatgc ggccgaatcg
     1321 cgcctagaat tccagcgatc atgcgagaag tgccgaaccg acgcgtccac accgtgatcc
     1381 tcggcggggg ccagggcaag cgcctcttcc ccctcacgcg cgaccgcgcc aagccggccg
     1441 tcccgctcgg cgggaagtac cggctgatcg acgtcccgct ctcgaacgcg atcaactcgg
     1501 gcttccggga catcgcggtc ctgacgcagt tcaactccgc ctcgctgaat aaccacatct
     1561 ccctgacgta ccgcttcgac agcttcgccc ggggccaggt ggagctcatc gcggcgcagc
     1621 agaccgacga ggggggggag tggttcgagg ggacggcgga cgccgtgagg aagcacctcc
     1681 ggcggctcgg cacgcgggac tacgaccacg tcctgatcct ggcgggggac cacctctacc
     1741 ggatggacta ccgggagatg ttcgacgccc acgtccagtc gagcgccgac gtgacggtcg
     1801 gcgtcctccc ggtcggggtc gaggagtgct cggggttcgg gatcctcctg accggctccg
     1861 acggccagat ccgcgccttc cgcgagaagc cgaagacgga ggaggagctc gccccgctcg
     1921 cccccgccaa ggagctgcgc gaccggtggg gccttcagcc cgggcagtac ctggcgagca
     1981 tgggggtcta cgtcttccgg atggagacgc tccgcgaggc gctcgcgaaa cccgcgaaca
     2041 tggacttcgg caaggacatc atcccgggcc acatcgggac ccacaaggtc caggccttcc
     2101 cgttccgggg ctactggcgc gacatcggga cgatccggag cttctacgag gcgaacatcg
     2161 ccctgacgga cgacgatccg ccgttccact tccaccaccc gaacgcaccg atcttcacgc
     2221 gcccgcgctt cctcccctcc tcctcgctca tcgacgtgaa ggtcgaccac tgccgcctgg
     2281 cggacggggt gaggatctac ggcgccgagg tgacgcgcag cgtcatcggc ctccgcgccc
     2341 ggatcgagaa ggggggccgg atcgtcgagt cgatcctgat gggcgcggac gagttcgagg
     2401 acgaggacct cgcggtcagc gccctcaacg aggggctccc gccggtcggg atcggggccg
     2461 actgcgtcat cgagagggcc atcgtcgaca agaacgcccg gatcggcgcg gggtgcgtcc
     2521 tgcgtggcga cccgtcccgc cccgaccagg acggggacgg ctggtgcgtc cgcgacggga
     2581 tcgtcatcgt cccgaaggac gccgtcctgc gacccgggac cgtcgtctga acccgggtcc
     2641 gggggacgga cgttccgtcc cccgggcccg ttctcgtcct tcgggaagga gcccgtgccc
     2701 cccgtctcct tcgtcctcgt cctccacggc cacatgccgg cggtcctcgg ccacggggtc
     2761 tggccgcacg gggccgactg gctgcacgag gcggcggccg agacgtacct cccgttcgtc
     2821 gccgccgccc gcgagctcga cgccgagggc gtgccgtggc gggcgacgat cggcctgacg
     2881 ccgatcctct gcgagcagtt gcgcgacccg cgcttcccgc gctcgttccg ccggtacgtg
     2941 acgcaccgac tccgggcggc ggggcgcgac gcgcgcgagt tcgcgcggga cggccatgtc
     3001 gccgagccgc tcgcgcacgc ctggcgcgcc cactacgagg aggggctcgc gctcttcgac
     3061 gcgtggggcg gggacctcgt cgccccgttc gtccggctgg cggaggcggg gcggatcgaa
     3121 gcgatcgcct cggcggccac gcacggcttc ctcccgctcc tcccgtccga cgcggccgcc
     3181 gagcggcagg ttgacgtcgg cctcgcggct caccgtcgcc acttcggccg ggccgcgcac
     3241 ggcttctggc tccccgagtg cgcgactgcg ccggccggga cgctcgtgac gcccgggcac
     3301 ggccggcggg tcgcgcgccg ctcggtcccg tccctcctcg cgtcgcgggg gctcgccttc
     3361 acgttcgtcg acacgcacct cgcggccggg ccggccgcgg gaacggccgc tggcggcagc
     3421 gcggggcgcc gctggccggc ggcggggggg agcccggcgg cgcgctcccc ctaccgggtc
     3481 caccgcgtcc ggaccgacgg cgggagcctc tccctcctcg cgcgcgaccc gcgcagctcg
     3541 gagcaggtct ggtcgggcga ggtcggctac ccgggagacc cggcctacct cgagttccac
     3601 cggaaggcgg cgcgcggggg cctccgctac tggtcggtga cggacgcgcg gggaccgctc
     3661 gacgccaagg agctctacga gcccgaagag acgcgcgtgc gcgtcgcgac ccacgcgtcc
     3721 cacttctgcg acctcctcga ggagacggcg cggatggaga gcgatgcccg cgtcgtcgtc
     3781 gcgatgttcg acttcgagct cttcgggcac tggtggttcg aaggggtcgc gttcctcgcc
     3841 gaggtcttcc gggagctggc ccgcaggaac gggccggtcc gggcggcgac cgcgggagag
     3901 gcggtgcggg aggcgggagc cgacgcgccc gaaatcgagc cggggcccgg ctcatggggg
     3961 cgaggtggcg actttcgcgt ctgggacgac gcgaagacga agccgttctg gcggagcgtg
     4021 gaggaggcgg aggcgcagct gcgccgcatc gaagcgcgcg atccgcggct cctgccgacg
     4081 gcgctccggc aggcgctcct cctccaggcc tccgactggc cgttcctcgt cgaagccggg
     4141 acggcgcggg attacgccca gcggaggatc gaggggcacg ccggggcgct ccgggcgctc
     4201 gcggagctcg cggggcgccc gaggagccgc gtggacgcgc tcttcctcgc gcgactcgcc
     4261 cgattcgatc gcgcctttcg gccggagctc gggggctgac ccgggcccgg gcgggaatcc
     4321 gtcggccgac gcgcaacgta gaatccgaga cgttccgtca gacatacccc gagaaaggac
     4381 gacccatggc ctacacggcg aagagctacg accacctcct cggcaccccg ggcttctccg
     4441 acgccctcat gaagaaccac ttcacgctct accagggcta cgtcacgaac acgaacaagg
     4501 tgaacgacga gctcgccgcc ctcgccgccg atgggaaggc cgccaccccc gccttcgccg
     4561 agatgaagcg ccgcctcggc tgggagtgga acggcatgcg gctccacgag tactacttcg
     4621 gcaacatgaa gaacgggggc gcggccctcg acggcggctc cgcgctcgcg aagaagctcg
     4681 cggccgactt cgggtcggtc gaggcgtggg agaagaactt ccgggcggtc ggcgcgatgc
     4741 gcggcatcgg ctggacggcg ctctaccagg accccgtctc cggccagctg atgaacatgt
     4801 ggatcggcga gcacgacggc ggccacccgg ccggcgggac gctcctgatg atcatggacg
     4861 tcttcgagca cgccttcatg ctcgactacg gcctgaagcg ccccgactac atcgaggcgt
     4921 tcttcaaggc gatcgactgg tcggtcgtct cggcccgctt cgacgcggcg ccgaagctcg
     4981 cgctgtaaga cggcccagga cggacctcgg acgacgggcg ggcttcggcc cgcccgtcgt
     5041 gtctccacgg gggggcgagt gacgtcgtgt tgccgggcac tcggccctcc ggcgcgggtc
     5101 gaccctctac agcgcccgcc aggcgtagcc cgttccgtcg cgcgtgaagg cggccgcttc
     5161 gaggagcggc gctacggccg agccggctgc ggggcgcggg gcggcgcccg acgcgaccgt
     5221 cgcaacgacg aggcgtcggg gtcggccgag cgggtcgcgg ccgcgccgca gaagctcctg
     5281 gagctcggcg agcgcggcgc ggaggacgcg gtcgggcgcc ggctcgcccc cgaggtcgag
     5341 cgccccggcg cccgagagcg tcgcgaccgg gacgccccgc gagaggacgc accagccgtc
     5401 gcgcgggact tcgacgccga cgagcaccgg gtcgacgccc gcgagcgcgg ccagctcggc
     5461 gcgcgggtct tcctcgtcgc gcgcctcccg catccgccgg agctcctcga cgacgtcggg
     5521 ctccgcgtac tggagcgggc cgctcccctc gacgaactca ccgcggcgca cgtcgccgcg
     5581 gagctccatt cgcgcgaagg ccggggcgac gtcgctccag cggacggcgc tctcggcctc
     5641 ctcgacgacg ccgcgcgaga cgacgcccca gcgggcgagg aggagtcgcg cccacgcctc
     5701 ggagcgctcc ccgggggcca gctcgggacc gaggacggac ggggcgccga ggagcgccca
     5761 gcgcccggag aggacgtgcg aggcggtggc cgggccgcgg ggcgtgaagc cgaggcgcgt
     5821 ggcgacgcgg gcctcggcgg cgcgcagctc gcgccgcgac gggacggctc cgagccgggc
     5881 cgggggcgcg gcgggagccg gcgcgatgcg cttcgcccgg aggagctcgc ggaggccgtg
     5941 gaagccgtcg cccgagacga gcccggcgag gaggagctcg cggagcgggg cgacgagcgc
     6001 ctccgggcgc ttcccgagcg ctccgccgag gtcggagagg aacgaggcgc cgccgaggcc
     6061 gagcgcctcg agaagagcac gcgcgtcggc cgagagggcg tcgaggccgg ccggtgcctc
     6121 ggggaggacg aagcgcccct ctccgcggaa gaagagcgac atccgcgcgg ccttcagctc
     6181 cttctccccc gcgaggcgga agacgaggag gccgcgggcg gcgaggacgt cgagggcgtc
     6241 gggctccggc tcggcgaggc gcgctgggag ggcggacgcc tcccacgagg cgacggggag
     6301 cgcgaggccg cggaggaggc cgacggcgcg ttcggcggcg tcgggcccgg cgaagcgcga
     6361 accggcgacg gccccctgcc gccgcgcgac gaacgcgcgg aggacggaac gcggcacggg
     6421 gcgaatctcg ccgcgcagga tcccgagcgt ccgccgccgg atcgcctcgg cgagccgcgc
     6481 ccccgcgaag cggcgttccc cgggaggacc gccgagccgc ccggcgacga cgagcgcctc
     6541 caggcgcagc cgctcgagcg agtcgaggac gtcgtcctcc gcgaggccgt agcgcgcggc
     6601 gacctcggca gccgtcaccg gtccccgtgt cccgacgtac ccgaggacga gccggtcgcg
     6661 ctcctcgcgg gagagcgtgc cggaggccag agcctcgaat cgggcgcgct cctccgccgg
     6721 gacgagccgc tcctcgccgc cgacgcgcat cctcacgatc gacccgcggg ccgtcagctc
     6781 ggcgacccac gcggcgccgt cccccgcgca gcgctcgatc gcctccgggg cggtcaggtc
     6841 ctggttctcc atcagggcga ggaggagctc ctcggccgtc cgcgccatcc gatgagggga
     6901 gagccgcgtc gcgtcggcgc gcgcggcctc cgcggcctcg gggcgcagcc cgtcgtcgag
     6961 gctctcgccg agcacctcgc cgaggagggc gcggttcgcg gcgacggcgt ggaggtcgcg
     7021 ctcggcgcgc ggcgcgtccc actcgtacat gtagacggcg acgaacttga agaggagcga
     7081 ggcggccgcg ggcgaggggg cccgccgctt ctcgaggagg cgcgcgaccg tccccttctc
     7141 catccggtcg aggaggccga gaaggcggtc catctcccag aggtcgcgga ggcagtcgcg
     7201 gtaggtctcg gcgacgacgg ggaagtcggg gagcgagcgc gtcgtctgga ggaggtcctt
     7261 cgccttgatc ctctggagcc agaagggcgt tctccgcccg ctccggttgc gcgggaggag
     7321 gagcgcgcgc tgggcgttct cgcggaagcg cgccccgaac atcggggagt tcgggagctg
     7381 cgcgaggagc cgctccttcg cctccgcggc ggatatccgc gacagccgct cgagcgggac
     7441 ctcgcgctcg gcgccgggaa tccggaggag gatcccgtcg tcggaggcga ccgtctcgac
     7501 gtcgaccccc gtctcctcgc ggaggacggc cgagaggacg agcgaccacg gcaggttcac
     7561 accgcgcccg aagagggacc tcacgacgat gcacgggtcg ccgaggtcgt tcgggaaggt
     7621 ctcgaagacg acggcgaggt cggaggcgag gccgcccgac tcggtcgcct cgcgccgcac
     7681 ggccccgacg atcgcggccg ttgcgtcggg cgtcgtcgca tagcggcgcg agacctcctc
     7741 gttcaggccc gggtcgtcga gccgctcggc gacgaaccgc cggagccgcc cgatccgctg
     7801 gccgaggtcg cccgtccgcc cgagcccctc gcccctccag aagggcatcc gcggggtcgg
     7861 ctgcccggga gccggctcca cgacgacggc gctggggccg atctcgacgg cgcgccagga
     7921 gcgcgacccg aggaggaaga cgtccccctc cttcgtctcg tggacgaact cctcgtccag
     7981 ctcgccgacg agcgtcttcc ggtcggcgag gacggcgcgg aaggcgccgc ggtcgccgat
     8041 cgtcccgccg tcgaggagcg ccgcgatccg cgcgcccggg aggggcgtga cgcggtcggt
     8101 cgcgcggtcc cagacgagct tcggggcgag ctcccggaac cgctcgcgcg ggtacttccc
     8161 cgagagcatc gcgagcgtct cgaggaagag cgagcgcggg agcgcgcggt aggggtacgc
     8221 cttccgcgcg agcgcgaaga gctcggccgc ggtgacggcg aggccgtcgg ccgcgcgcgc
     8281 gaccgcctcc gccacgagct gctgcgcgag gacgtcgagc gggaagtcgg gcggacgcgt
     8341 ctcctcgacc gccccttctc tcatggcctc ggccgtcgcg gcgctctcga agaggtcgtc
     8401 gaggtagagc gggacgaaga ggccgcgcgc cgtctcgccg acgaggtgcc ccgaccggcc
     8461 gacccgctgg agccctcgcg cgatcccctt cgggctctcg aggaggacga cggagtcgac
     8521 gtgcccgacg tcgatcccga gctcgagcga gcccgtcgcg acgaggaccg gcagcgtccc
     8581 cgccttcagc tccttctcga tctcctcccg ccgcgggcgc gagaccgagc cgtggtgcgt
     8641 cgggacgtcg gcgcccgtcg cgtccgagag cttgcgcgag accttctcgg cgagccgccg
     8701 gttgttgcag aagacgagcg tcgtcgtcct cgactccacc tccctcacga cggcctcgac
     8761 ggctggcccc caggccgagc cgtcggggag gtcgcggaag tcgggggcgg gggaggcgac
     8821 gacgaggtcg agcgccttct tcgcggggac cacgagcggc ttcgtcttcc ggcccgtccc
     8881 ggcggcgaac gaggcgacga cgtcgacggg cgcgaccgtc gccgacaggg cgacccgctg
     8941 cggcggggcg ccgccggccc gcgcgatcac ctcctcgagc cgctcgagcg agagggcgag
     9001 gtggctcccg cgtttcgtcc cggcgacgga gtggacctcg tcgaggacga cggtcgagac
     9061 cccctcgagc gcccggcgcc cgccgccggt cagcatcagg tagagcgact cgggggtcgt
     9121 gatgaggacg tcggggggcg ccttcagctg gctcgtccgc tcgcgggtcg gcgtgtcacc
     9181 ggtccgcacc gccgtcgtga tcgggacgcg cccggccccg agctgcgcgg cgatcccggc
     9241 gcgcggccgc tcgaggttcc ggtggacgtc gttgtcgagc gccttcagcg gcgagacgta
     9301 gagcgtcgcg aggcgcgggg cgtcgtcgcc ggcgagccgg cgcctcagga tcccgtcgag
     9361 ggcgacgagg aaggcggcga gcgtcttgcc cgtgccggtc ggcgagacga ggaggacgct
     9421 ctcgccgcga gcgatcgcgg gccaggcgag gcgctgcgcc tcggtcgggc cttcgggaaa
     9481 ggtctcgagg aaccaggcgg cgaccgcggg atggaagagg tcgagcgggt cggccgcggg
     9541 cccgctgccc cggcgggcgg tcaccgtcgt gccgaagccc ggcgccgcgc ctccccttcg
     9601 gcctcgcgga ggaacgcgtc gaggaggtcc cacgggtcct ccgcgagcct ctcgccgtcc
     9661 gccggcgcgg gtccgtggaa gacgagccgg acgacgacct cggccttccc gtcgaacggc
     9721 tccgccg
//
LOCUS       DAOONL010000128         9579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8296_length_9579_cov_13.427341, whole genome shotgun sequence.
ACCESSION   DAOONL010000128 DAOONL010000000
VERSION     DAOONL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8296_length_9579_cov_13.427341"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLP50_14275"
     CDS             complement(<1..611)
                     /locus_tag="PLP50_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA52758.1"
                     /translation="MRYGVAAAVLGLLASGAVRADGPLTLGEAMARARDGAREVAAAK
                     ARQEAALARARQASAFRLPSLTLSETYIRTSSPVDSFGLELNREEFTFQKFMTTDPND
                     PGWSGTAITRLEAQAPLFTGGELSGRIAQARSAADAASSQASWAADNAALAAGEAWVM
                     LAQAEEFVGLLTKARDTVAAHVDLAKSYVEQGMLVRSELLRAEV"
     gene            824..4273
                     /locus_tag="PLP50_14280"
     CDS             824..4273
                     /locus_tag="PLP50_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA52759.1"
                     /translation="MRTDEPADGGGSERYRRQRRIQEATYAIAHAVTTTAGLDDLFAR
                     IHRIVGGLMEARNLYIALLDEATGSLSFPYFRDEIDAEPPEGPVPAGRGLTGYVLRTG
                     EPLLASPEVFDDLVARGEVAPIGAASIDWLGVPLRAGDRTFGVLAVQSYAGNVRYGEE
                     DQALLGFVSSQVALAIERRRAEEALRLSEMRHRAVLEALPDLLFVLDREGRYLAVHAA
                     RPEALAAPRESLLGSSVPEILPKEAAEVWMDAIRRCLGGGGMQVIEYELDLQAGRRAF
                     EGRVVPHDADSVLSIVRDVTDRVERERELRERERELARLTENMIDVITETDLHGNLRF
                     ATPSYVAATGWSPEEFLGHNALEFIHPEDVELSREMLGRRARRDGSSAFTYRFRAKDG
                     RWLWVDSIVTLAKGAPGESDGLVITSRDVTGRKRDEEVVRLQHETERGILRREGPAVI
                     LARICTRVSELFDLPAVWIGLKEPDGRIEPRAVAGSASGFVTRSRFRWDDVPEGRGPS
                     GEAVRTGRPIFVDGLEDGRVAPWRDAAAETGLGAALSVPLVVGERVLGVLACYARGPE
                     AFAGAAVVPITRIADQVALSLLEAEQLERIELQTAALEATANAVVITDREGRVEWANR
                     AFTELTGFSAADLAGETPRVLKSGIQSEFFYERMWETILAGNVWRGELYNRRKDGTVY
                     VEEQTITPVRTADGGIRHFVAVKQDVTQRRRTEERIRYLALHDPLTDLGNRRAAEESL
                     ERIVARARRGTPGSLLLLDLDHFKVVNDTLGHGAGDVVLVELARLLGTLRRPGDELAR
                     LGGDEFVLVLEGIPAEAGRMVAERVRRAVHEHRFEVGGRRFDLGVSVGVVPIDGRLKP
                     AALLALADSALYAAKEKGRNRVMLFDGSVQPTPLSEASLWASRVKDALRERKFVLVYQ
                     PILRLENGRTAHYEALLRLSDGDGALVAPGDFLPAAEQFGLLPQVDAWVVDEVLSVLR
                     TRNDVEVFVNLSGASLGEEGHLAALEERIRESGIGPGRLAFELTETTAVRDLIGAREW
                     MRRLRDLGCRFALDDFGIGFSSFSYLQGLPADYVKIDGSFIRDLETNPSNRALVKAID
                     TVAHTLGKETIAEHVESLDAVAVLRELGVEYGQGFALGLPERELPGLPDARDAATSPG
                     TLR"
     gene            4319..6556
                     /locus_tag="PLP50_14285"
     CDS             4319..6556
                     /locus_tag="PLP50_14285"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPA52760.1"
                     /translation="MPSESPRRVAILTSGGDAPGMNAVVRAAVRAALERGLEVLAVEE
                     GFQGLVDGGERFRRMAWGDVGGILPKGGTVIGTARCPAFRTREGRLAAARNLARERVE
                     ALIVVGGDGSLTGADLFRREWPSLLKELAAGGEIPPEAARSASPLALVGLVGSIDNDM
                     AGTDMTIGADTALHRIVEAVDALVSTAASHQRTFVVEVMGRHCGYLALMSALATGAGW
                     VFIPEAPPEGDDWEEKLCAVLAEGRKAGRRHSTVILAEGAVDRQGRPIEAERIRKLLE
                     ERLGTETRTTVLGHVQRGGAPSAFDRTMGTLLGVAAIEEVVRWGPDDVPCLIGLRENR
                     VTRVPLLENVEKARAVGEAIRAGDFERAMTLRGTSFRSSFRIMKTLVRAFPHGPRDGQ
                     RRRRFLVLHAGPPAPGMNTAVRAAVRLLVDQGHVVLGARGGFDGLLAGDVVPLDWMSV
                     NGWVSLGGAELGTSRRVPAAEDLGALAEVLAARQVEGVLLVGGWAAYQAAGRMQAARA
                     EHAAFRVPLVCVPAGIDNSLPGSELAIGADTALNAIVEAVDRIKQSAVAWKRTFVVEV
                     MGRWCGYLAVLSGLATGAERVFTHEEGITMKALERDVASLVEGFRKGKRLALMIRNEN
                     ASPVFTTGFLRRLFEAEGRGAFDARQAILGHLQQGGDPTPFDRIVATRYAARAVEVLL
                     EESAKESPAAVFLGVQSGRLTAFPLDALASMVEPEFERARDPWWRSIEPVVRLLGQPG
                     PSAKA"
     gene            complement(6558..7901)
                     /gene="pfp"
                     /locus_tag="PLP50_14290"
     CDS             complement(6558..7901)
                     /gene="pfp"
                     /locus_tag="PLP50_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPA52761.1"
                     /translation="MTTSQHKNMAILVGGGPAPGINSVISAATIRAELEGVSVLGIRD
                     GFQWLMQGNIEHVHPLTIDEVSRIHFRGGSHIGIARANPTKDPQLLEAAVISLLRLNV
                     SRLVTIGGDDTAFSAMKLAEKADGRIAVVHVPKTIDNDLDLPPDVDTFGFQTARHVGV
                     EIVKNLMVDARTTSRWYFVIAMGRKAGHLALGIGKAAGATLTLIPEEFQGQKVRFKAI
                     VDTLVGAILKRLSYGRRDGVAIIAEGLVLELDPEELKGLAEAERDAHGHLRIADVNIG
                     EILKAAVQEELKKLGVKATVTDKNIGYELRCADPIPFDLEYTRDLGYCAAKYILSGGT
                     GAMVSMQGGNFVAIPFAEMLDPQTGRTRVRMVDVHSTRYAIARRYMIRLRKDDFEDPH
                     ELAKFAATCGMSLADFRARFGYLVENELPPIVLLPSGHRDETGPGSGSYFEGERL"
     gene            complement(7973..>9579)
                     /locus_tag="PLP50_14295"
     CDS             complement(7973..>9579)
                     /locus_tag="PLP50_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52762.1"
                     /translation="FLLAPLAGVLLAAAAAGGIARLAGASTSLQGLAILLASLGGPLG
                     SYATSDFSEALQAAALAGALLFAFGAAREDDPRASRRRAFAAGLAAGAALLVKSSLVA
                     ASPLLLLPLLAASALRGRRLAAAAAGAILPVAAWAAFELHRFGRLFSGYEGEGFTHPL
                     PEGLWRLLVGPNRGLVLFFPAALLAAAGLARAARRPGDTPLRLAAAGSALAFLALLGT
                     AAAWWAWHGVGGWGPRLLVPAVPLLAPWAALAAEGLGTGGRRAVAVLSVALNLPPLLV
                     HPSLVDTYVANCRRAPLTPELEREVPALAVGPDDAGRPSVSPENVLAKEPLASPFLLY
                     PWYAAASAPRSAEGRAERLARPPWYETRPGLGPRLVPFPPELADVVAPPLGWSFFGRA
                     LGRGIADPASFSVYLAALSEQVGRAHETGRLERGLELARKLARLDPGEGSDALLLESL
                     RLLRRTEALRAAFASLPEERVSGPSVLVVRALAARDAGDLPLAARLGEAVAPYFPGTA
                     LARDAARPSSWPATFAALTRPLAPSA"
BASE COUNT         1299 a         3335 c         3716 g         1229 t
ORIGIN      
        1 acctcggccc ggaggagctc ggatcggacg agcatcccct gctcgacgta gctcttcgcg
       61 aggtcgacgt gggcggcgac ggtgtcgcgg gccttcgtca ggaggccgac gaactcctcg
      121 gcctgcgcga gcatcaccca cgcctcgccg gccgcgaggg cggcgttgtc ggcggcccag
      181 gaggcctggc tcgacgcggc gtcggccgcg ctgcgcgcct gggcgatccg cccggagagc
      241 tcgcccccgg tgaagagcgg cgcctgggcc tcgaggcgcg tgatcgccgt ccccgaccag
      301 cccgggtcgt tcgggtcggt cgtcatgaac ttctggaacg tgaactcctc gcggttcagc
      361 tcgaggccga acgagtcgac gggggagctc gtccggatgt acgtctccga gagggtgagg
      421 gaggggagcc ggaacgccga cgcctgccgc gcccgggcca aggccgcctc ctgccgggcc
      481 ttcgcggcgg ccacctcgcg ggcgccgtcg cgggcgcggg ccatcgcctc cccgagggtg
      541 agcggtccgt ccgcgcggac ggcgcccgag gccaggagcc ccaggaccgc cgccgccact
      601 ccgtatcgca tgtctcctcc ttgccggcgg gggcggaacg cccccgatgt gtgactatat
      661 gctaatatag ttatattaga tccggctgtc aagccccgtc tccgcgcgcc gtcgttccgc
      721 gccggagcgc gggtcggagg agggggcccg tgggtatcgg ccgattctcc tccccggagc
      781 gaaagccggg ggcaccggct aacatccacc ctccggtgcg cgcatgagga cggacgagcc
      841 cgcggacggc gggggatcgg agcggtaccg ccgtcagagg cggatccagg aggcgaccta
      901 cgccatcgcc cacgcggtga cgacgaccgc cggcctggac gacctgttcg cccggatcca
      961 ccggatcgtc ggcggcctga tggaggcgcg gaacctctac atcgcgctgc tcgacgaggc
     1021 gaccgggagc ctctccttcc cctacttccg ggacgagatc gacgcggagc ctcccgaggg
     1081 gccggtcccg gcggggaggg gcctgacggg ctacgtcctc aggaccggcg agcccctcct
     1141 cgcctcgccg gaggtcttcg acgacctcgt cgcgcggggc gaggtggcgc cgatcggcgc
     1201 ggcctcgatc gactggctcg gcgtgccgct tcgggccggg gaccggacgt tcggcgtcct
     1261 cgccgtccag agctacgcgg ggaacgtgcg ctacggggag gaggaccagg ccctcctcgg
     1321 cttcgtctcg tcccaggtcg cgctcgccat cgagaggcgg cgggccgagg aggcgctccg
     1381 gctgagcgag atgcgccacc gggccgtcct cgaggcgctc cccgacctcc tcttcgtcct
     1441 cgaccgcgaa gggcggtatc tcgccgtcca cgcggcccgg ccggaggcgc tggccgctcc
     1501 tcgcgagagc ctcctgggat cctccgtccc cgagatcctg ccgaaggagg cggccgaagt
     1561 ctggatggac gccatccggc gctgcctcgg cgggggcggg atgcaggtga tcgagtacga
     1621 gctcgacctg caggccggac gccgggcgtt cgaggggcgg gtcgtccctc acgacgcgga
     1681 ctcggtcctc tccatcgtcc gcgacgtgac ggaccgcgtc gagcgcgaac gcgagctccg
     1741 cgagcgggag cgcgagctcg cgcgcctgac cgagaacatg atcgacgtca tcaccgagac
     1801 ggacctgcac gggaacctcc gcttcgcgac gccctcgtac gtcgcggcga cgggctggtc
     1861 ccccgaggag ttcctcgggc acaacgccct cgagttcatc cacccggagg acgtggagct
     1921 gtcgagggag atgctcggcc ggagggcgcg ccgcgacggc tcgtcggcgt tcacctacag
     1981 gttccgagcg aaggacgggc gttggctctg ggtcgactcc atcgtcaccc tggcgaaggg
     2041 ggcgcccggc gagtccgacg gactcgtcat cacgagccgc gacgtcacgg ggcgcaagcg
     2101 ggacgaggag gtcgtgaggc tccagcacga gacggagcgc gggattctcc ggcgcgaggg
     2161 gccggccgtg atcctcgccc gcatctgcac ccgtgtctcc gagctgttcg acctcccggc
     2221 cgtctggatc ggcctgaagg agcccgacgg aaggatcgag ccgcgcgccg tcgcggggag
     2281 cgcctcgggc ttcgtgaccc gctccaggtt tcgctgggac gacgtgccgg aaggccgcgg
     2341 gccgtccggc gaagcggtcc ggaccggtcg gcccatcttc gtcgacgggc tggaggacgg
     2401 ccgcgtggcg ccgtggcggg acgccgcggc ggaaacgggc ctcggcgcgg cgctctcggt
     2461 gccgctcgtc gtcggcgagc gggtcctcgg ggtccttgcc tgctacgcgc gtgggccgga
     2521 ggccttcgcg ggagcggccg tcgtcccgat cacgcggatc gcggaccagg tcgctctctc
     2581 tctcctcgag gcggagcagc tcgagcggat cgagctccag acggccgccc tcgaggcgac
     2641 ggcgaacgcc gtcgtcatca ccgaccgcga ggggcgcgtc gagtgggcga accgcgcctt
     2701 cacggagctg acgggcttct cggccgcgga cctcgccggg gagacgccgc gggtcctgaa
     2761 gtcggggatc cagagcgagt tcttctacga gcggatgtgg gagacgatcc tggccgggaa
     2821 cgtctggcgc ggggagctct acaaccggcg gaaggacggg accgtctacg tcgaggagca
     2881 gacgatcacc ccggtgcgaa ccgccgacgg cgggatccgg catttcgtcg ccgtgaagca
     2941 ggacgtgacg cagcggcgcc ggacggagga gcggatccgc tacctcgcgc tgcacgaccc
     3001 gctcacggac ctcgggaacc ggcgcgccgc ggaggagagc ctcgagcgga tcgtggcccg
     3061 ggcgcgccgc gggacgcccg gctcgctcct cctcctcgac ctcgaccact tcaaggtcgt
     3121 caacgacacg ctcgggcacg gtgcggggga cgtcgtcctc gtcgagctgg cgcggctcct
     3181 cgggacgctg cgccggccgg gcgacgagct cgcgcgcctc gggggcgacg agttcgtcct
     3241 cgtcctcgaa gggatcccgg ccgaggcggg acgaatggtc gccgagcgtg tccgccgggc
     3301 cgtgcacgag catcggttcg aggtgggggg gcgccggttc gacctcggcg tgagcgtcgg
     3361 ggtcgtcccg atcgacggcc gcctcaagcc cgccgcactc ctcgccctgg ccgactcggc
     3421 gctctacgcc gccaaggaga agggccgcaa ccgcgtcatg ctgttcgacg gctccgtgca
     3481 gccgacgccc ctctcggagg cgagcctctg ggcctcgcgc gtgaaggacg cgctccgcga
     3541 aaggaagttc gtcctcgtct accagccgat cctgcggctc gagaacggac ggaccgcgca
     3601 ttacgaggcg ctcctccgcc tctcggacgg cgacggggcc ctcgtcgccc cgggggactt
     3661 ccttcccgcc gcggagcagt tcggcctcct cccccaggtc gacgcgtggg tcgtcgacga
     3721 ggtcctctcg gtcctcagga cccggaacga cgtcgaggtc ttcgtcaacc tctccggcgc
     3781 cagcctcggc gaggaggggc acctcgccgc gctcgaggaa cggatccggg agagcggcat
     3841 cggccccggg cgcctcgcct tcgagctgac cgagacgacc gccgtccggg acctgatcgg
     3901 ggcccgcgag tggatgaggc gcctgcgcga cctcggctgc cgcttcgcgc tcgacgactt
     3961 cgggatcggc ttctcgtcgt tctcctacct gcaggggctg ccggcggact acgtcaagat
     4021 cgacggctcg ttcatccgcg acctcgagac gaacccctcg aaccgggccc tcgtgaaggc
     4081 gatcgacacc gtcgcccaca cgctcggcaa ggagacgatc gccgagcacg tggagagcct
     4141 ggacgccgtg gccgtcctcc gggagctggg cgtcgagtac gggcaggggt tcgcgctcgg
     4201 cctgccggag cgggagctgc ccggcctccc ggatgcccgc gacgcggcga cgtcgcccgg
     4261 cacgctccgt taggatgtcc ggtcgcgggc cggcgcagcc ggcccggagg agacgaggat
     4321 gccgagcgag agcccgcgcc gtgtcgcgat cctgacgagc ggcggcgacg ccccgggaat
     4381 gaacgccgtc gtgcgggccg cggtccgcgc ggcgctcgag agggggctcg aggtcctcgc
     4441 cgtggaagaa ggcttccagg ggctggtcga cgggggagag cgattccgcc ggatggcctg
     4501 gggcgacgtc ggcggcatcc tcccgaaggg ggggaccgtc atcggcacgg cgcgctgtcc
     4561 ggcgttccgc acccgcgaag gtcgtctcgc tgccgcccgg aacctggccc gcgagagggt
     4621 cgaggccctg atcgtcgtcg gcggggacgg gtcgctcacg ggcgcggacc tcttccggcg
     4681 cgagtggccc tctctcctga aggagctcgc cgccggcggg gagatcccgc ccgaggcggc
     4741 gaggagcgcg tcgcctctcg cgctcgtcgg cctcgtcggg tcgatcgaca acgacatggc
     4801 cgggaccgac atgacgatcg gcgccgacac ggccctgcac cggatcgtgg aagccgtgga
     4861 cgcgctcgtc tccaccgccg ccagccacca gaggaccttc gtcgtggagg tgatggggcg
     4921 ccactgcggt tacctggcgc tgatgagcgc gctcgcgacg ggcgccgggt gggtcttcat
     4981 ccccgaggcg ccgccggagg gggacgactg ggaggagaag ctctgcgccg tcctggccga
     5041 ggggcggaag gcgggccgcc gccactcgac ggtgatcctg gccgaagggg cggtcgaccg
     5101 gcaggggcgc cccatcgagg cggagcggat ccggaagctc ctcgaggagc ggctcgggac
     5161 ggaaacgcgg acgaccgttc tcggccacgt ccagcgcggc ggcgccccga gcgccttcga
     5221 ccggacgatg gggacgctcc tcggcgtcgc ggcgatcgag gaggtcgtcc gctgggggcc
     5281 cgacgacgtc ccgtgcctca tcgggctccg cgagaaccgc gtgacgcgcg tgccgctcct
     5341 ggagaacgtc gagaaggcgc gggcggtcgg cgaggcgatc cgggcgggcg acttcgagcg
     5401 ggcgatgacg ctccgcggga cgtccttccg gagctccttc cggatcatga agacgctcgt
     5461 ccgtgccttt ccccacgggc cgcgcgacgg tcagcggcgg aggcggttcc tcgtcctcca
     5521 cgccggaccg cccgcgccgg gaatgaacac cgccgtgcgc gcggcggtcc gcctcctcgt
     5581 cgaccagggg cacgtcgtcc tcggcgcgcg cggcggcttc gacgggctcc tcgccggcga
     5641 cgtcgtgcct ctcgactgga tgagcgtgaa cggctgggtc tcgctcggcg gcgccgagct
     5701 cgggacgagc cgcagggtcc cggcggcgga agacctcggg gcgctcgccg aggtgctcgc
     5761 ggcgcgccag gtcgaaggcg tgctcctcgt cggcggctgg gcggcctacc aggcagcggg
     5821 ccgaatgcag gccgcgcgcg cggagcacgc ggccttccgc gtcccgctcg tctgcgtccc
     5881 ggcggggatc gacaactccc tccccggctc cgagctcgcg atcggcgccg acacggcgct
     5941 gaacgcgatc gtcgaggcgg tggaccggat caagcagtcg gccgtggcct ggaagcgaac
     6001 gttcgtggta gaggtcatgg ggcgctggtg cggctacctc gcggtcctct ccggactcgc
     6061 gaccggcgcc gagagggtct tcacgcacga ggaagggatc acgatgaagg cgctcgagcg
     6121 cgacgtggcg agcctcgtcg aggggttccg gaaggggaag cgtctcgcgc tgatgatccg
     6181 gaacgagaac gccagccccg tcttcacgac gggcttcctc cggcgactgt tcgaggcgga
     6241 gggccgaggc gcgttcgacg cgcggcaggc gatcctcggg catctccagc aggggggcga
     6301 cccgaccccc ttcgaccgga tcgtggcgac ccgctacgcg gcccgggcgg tggaggtcct
     6361 cctcgaggag agcgcgaagg agagcccggc ggccgtcttc ctcggcgtcc agtcgggccg
     6421 cctgacggcg ttcccgctgg acgccctcgc gtcgatggtc gagccggagt tcgagcgcgc
     6481 cagggacccg tggtggcgct cgatcgagcc cgtcgtccgg ctgctcggcc agccggggcc
     6541 ctcggcgaag gcctgagcta cagcctctcc ccctcgaagt acgagccgct cccggggccg
     6601 gtctcgtcgc gatggcccga gggcaggagg acgatcggcg ggagctcgtt ctcgacgagg
     6661 taaccgaagc gggcgcggaa gtctgcgagc gacatgccgc acgtcgcggc gaacttcgcg
     6721 agctcgtgcg ggtcctcgaa gtcgtccttg cggagccgga tcatgtagcg acgcgcgatc
     6781 gcgtagcggg tcgagtggac gtcgaccatc cgcacacgcg ttcggccggt ctgcgggtcg
     6841 agcatctcgg cgaaggggat cgcgacgaag ttcccaccct gcatcgagac catcgcgccc
     6901 gtccctcccg agaggatgta cttggccgcg cagtagccga ggtcgcgcgt gtactcgagg
     6961 tcgaacggga tcgggtcggc gcagcggagc tcgtagccga tgttcttgtc ggtgaccgtc
     7021 gccttcacgc cgagcttctt cagctcctcc tggacggccg ccttcaggat ctcgccgatg
     7081 ttcacgtcgg cgatccggag gtgaccgtgg gcgtcgcgct cggcctcggc gagccccttc
     7141 agctcctcgg ggtcgagctc gaggacgagc ccctcggcga tgatcgccac gccgtcacgc
     7201 cgcccgtagc tgaggcgctt caggatcgcg ccgacgagcg tgtcgacgat cgccttgaac
     7261 cgcaccttct gcccctggaa ctcctccggg atcagcgtga gggtggcgcc ggcggccttg
     7321 ccgatgccga gtgcgaggtg gccggccttc cgtcccatcg cgatgacgaa gtaccagcgc
     7381 gaggtcgtcc gcgcgtcgac catcaggttc ttgacgatct cgacgccgac gtgccgcgcc
     7441 gtctggaagc cgaacgtgtc gacgtcgggc gggaggtcga ggtcgttgtc gatcgtcttc
     7501 gggacgtgga cgaccgcgat ccgcccgtcg gccttctcgg cgagcttcat cgccgagaag
     7561 gcggtgtcgt cgccgccgat cgtgacgagc cgcgagacgt tcagccggag gagggagatg
     7621 acagcggcct cgaggagctg cgggtccttc gtcgggttcg cgcgggcgat gccgatgtgc
     7681 gagccgccgc ggaagtggat ccggctcacc tcgtcgatcg tgagcgggtg gacgtgctcg
     7741 atgttcccct gcatgagcca ctggaagccg tcgcggatgc cgaggaccga gaccccctcg
     7801 agctcggcgc ggatcgtcgc cgcgctgatg acgctgttga tgccgggcgc cggcccgccg
     7861 ccgacgagga tcgccatgtt cttgtgctgg ctcgtggtca tgggacctcc gtgaggcggg
     7921 gaagtctacg gcagcggaag ggggagggag gaccccgcgg gcccgcggtc cctcacgcgg
     7981 acggcgcgag cgggcgcgtg agcgccgcga acgtcgcggg ccaggaggac gggcgggcgg
     8041 cgtcgcgggc gagcgcggta ccggggaagt agggcgcgac ggcctcgccg agccgcgcgg
     8101 cgagggggag gtccccggcg tcgcgcgcgg cgagcgcgcg gacgacgagg acggaggggc
     8161 cgctcacgcg ctcctcgggc agggaagcga acgccgcgcg gagcgcttcg gtccggcgga
     8221 gaagccgcag gctctcgagg aggagggcgt cggaaccctc cccggggtcg aggcgtgcga
     8281 gcttccgggc gagctcgagc ccccgttcga gccgtcccgt ctcgtgcgcc cggccgacct
     8341 gctcggagag ggcggcgagg tagacggaga acgacgccgg gtccgcgatc ccccgcccga
     8401 gcgcacggcc gaagaagctc cacccgagcg gcggcgcgac gacgtcggcg agctccggcg
     8461 ggaacggcac gaggcgaggc ccgagcccgg gccgcgtctc gtaccagggg gggcgggcga
     8521 gccgctccgc ccggccctcc gcggaacgcg gcgccgaggc ggccgcgtac cagggataga
     8581 ggaggaacgg cgaggcgagc ggctccttcg cgaggacgtt ctccggggag acgctcggcc
     8641 tccccgcgtc gtcggggccg acggcgaggg cggggacctc gcgctcgagc tccggggtga
     8701 gcggtgcgcg ccggcagttc gcgacgtacg tgtcgacgag cgaagggtgg acgaggagcg
     8761 gcggcaggtt gagcgccacg gagaggaccg cgacggcgcg ccggcccccg gtgccgaggc
     8821 cctcggcggc gagcgcggcc cacggcgcga ggagcggcac ggcggggacg aggagacgcg
     8881 gtccccagcc cccgacgccg tgccaggccc accaggcggc ggcggtcccc agcagggcga
     8941 ggaacgcgag cgcggagccg gcggcggcga ggcggagggg cgtgtcgccc gggcggcgcg
     9001 cggcccgcgc caggcccgcc gccgcgagga gcgcggccgg gaagaagagg acgaggccgc
     9061 gattcggccc gacgaggagc cgccagaggc cttcggggag cgggtgcgtg aagccctccc
     9121 cttcgtagcc cgagaagagg cggccgaagc ggtggagctc gaaggcggcc caggcggcga
     9181 cggggaggat tgcgccggcc gcggcggccg cgagccgccg cccgcggagc gcggacgccg
     9241 cgaggagcgg gaggaggagg agcggcgagg cggcgacgag gctcgacttg acgaggagcg
     9301 ccgccccggc ggcgaggccc gccgcgaacg cgcggcgccg cgaggcgcga gggtcgtcct
     9361 cccgagcggc gccgaaggcg aagaggaggg cgcccgcgag cgcggccgcc tggagcgcct
     9421 cggagaagtc ggaggtcgcg tacgacccga gaggcccgcc gagcgaggcg aggaggatcg
     9481 cgagcccctg gagcgaggtc gaggctccgg ccagccgggc gatgccgcct gcggcggccg
     9541 ccgcgaggag gacccccgcg agcggcgcga ggaggaaga
//
LOCUS       DAOONL010000129         9435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8480_length_9435_cov_19.264499, whole genome shotgun sequence.
ACCESSION   DAOONL010000129 DAOONL010000000
VERSION     DAOONL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8480_length_9435_cov_19.264499"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /gene="mfd"
                     /locus_tag="PLP50_14300"
     CDS             <1..1931
                     /gene="mfd"
                     /locus_tag="PLP50_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPA52763.1"
                     /translation="LLVPVERLDLLQKYQSGGEGPAPPLDKLGGAGWAKRKASVRKAV
                     KDIAEQLLKLYARRATTPGHSFSKDSPWQKEFEEAFEFTETPDQLSAIRDIKRDMESE
                     RSMDRLLCGDVGYGKTEVAMRAVFKCVLDGKQAAVLAPTTILADQHHRTFRRRFAAFP
                     VTIDLLSRFRTSDEQKEVVRRLAEGTLDVVVGTHRILSKDVSFRDLGLVVIDEEQRFG
                     VAQKEKLKELRASVEVLSMSATPIPRSLNLSLGGIRDLSVIETPPKDRLAISTHVVPQ
                     GDEVVREAIRAELDRGGQVYLVHNRIEGLGVWRERLAELVPECRVVVGHGQMSEGELE
                     RAMRAFTTRAADLLLATTIVENGLDIPTANTMIIDRADLYGLSQLYQLRGRVGRSDKP
                     ASCWLLVPPGAPLSDDARRRLRAIQEFSDLGAGFRIAAKDLEIRGSGNVLGGEQSGHI
                     DAVGFETYVTLLEEAIGELKGEPVAEKREVTLQLGLPLGLPKKWIGEESLRMALYKRL
                     ATAEGVDALDVLEKEATDRYGAPPPEFARLLGLGRLRLLALDLGVRSLQRRGAELAVT
                     LDKGHRLDPDRLLGAIRAKGLAASGPESFRAPELFRGLPPDTEEVCGVAGRFLVSLAR
                     PATAFAPGLLAAFSEGPR"
     gene            1928..2791
                     /locus_tag="PLP50_14305"
     CDS             1928..2791
                     /locus_tag="PLP50_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPA52764.1"
                     /translation="MRRGALVLVPLVAALALSGCARKGPRAAARTSVVAMVGDEPVEE
                     EDFENYVRAAAGAGLGQVSPQVASSLLDQYLEERLLERAVAEAEPSPRGKTAAERRAE
                     VLARAARLEQITDTELRAEYDAEPARWEHPPLVRLSQMILPDRAAAETARRRVLAGEA
                     WEKVSREASRAPNAASGGGLGWLSRTDLPSEFEKAIWGLPAGGVSPPQPAGHGVHLFR
                     VDERADGRVVPFEEARPALRLALSEKRSSEALERLLAEARKRHRVGVIEEHLPFPYVG
                     GAARFSLDSAP"
     gene            2788..3756
                     /locus_tag="PLP50_14310"
     CDS             2788..3756
                     /locus_tag="PLP50_14310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPA52765.1"
                     /translation="MTFHRRRPGTAVLALLAALALPAGAEVLEQILVHVNGRIVTQSQ
                     LDDRVRAVLAEAPPDLDQGRRDDLRRAALKELVDESLLEDRARDLDLVATDAEVEEQV
                     KHLKEQNNLASEEEFVRALAASNLTPAKLREQLKRTSTIQRVVGREVNSRVDLSDDAL
                     RLVYERQKETWAVPEKVRVSEILVNAGPGAEEKAREAASLVKGGARFEDVVVRFSDGP
                     TRSKGGDMGTFGRGELAAALDAAAFSLPAGGVSDPIETKSGWHVLKVVEKIPAGYRPF
                     AEAKGEILRKEQETQFQKKLAEYVEKLKGEAIIKYSDEARALMPQP"
     gene            3830..5917
                     /locus_tag="PLP50_14315"
     CDS             3830..5917
                     /locus_tag="PLP50_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52766.1"
                     /translation="MSDDPVPTVSREERYRALLGISNVLGVIRDPAELVGRLARELRQ
                     IATFDFMSLYLGDVPGRGPTWKVWRVDEQAIVAVPESIPLEETVEAWVAANRRVVAWP
                     PPAGQPSFPRLEAVLERHGIRSYLAVPLLSAGRPVGALTFGSFRPLEYDEAETEFLSV
                     VADQVALAIGGAASFERVRVAHERLERSSERLRLVLEVGSAVLANLDLEELLRAISAN
                     VRRVLRCDVASVILLDAERSRARLHVLDFPEGRGFVKEGAEIPVRRDAVEELLRSSRP
                     RELAVDREGTPGSDVARAEGIRHGWTFPLVSRGRALGFFTVGRRDETRFDADEVDLLA
                     QVAGQVATALDNALAYREIAGLKDQLAREKLYLEDEIRSEMSFDEIVGRSEALRRVLR
                     QVETVAPTDSTVLIHGETGSGKELVARAIHDRSARAGRTFVKLDCAAIPSGLLESELF
                     GHQKGAYTGAISDRVGRFELASGGTVFLDEIGEVPLELQPKLLRILQEREFERLGSSK
                     TLRTDARLIAATNRDLEAMVEEGTFRADLFYRLNVFPIHVPPLRERPEDVPLLVRHFV
                     QQLGRKTRKKVETIPSETMAALQRYDWPGNIRELQNLIERALILTTGPVLQVPLVGLK
                     SRSGAPPAIPTRTLREAEREHVLAVLESTGWVLGGANGAAARLGLNRSTLQFRLKKLG
                     LVRPDRGGAADRA"
     gene            6100..6408
                     /locus_tag="PLP50_14320"
     CDS             6100..6408
                     /locus_tag="PLP50_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52767.1"
                     /translation="MLRITIEPYEQEVRLKLEGDLIGTWVRDLEESWRAAREGGRDAG
                     LDLTGVTKVDDAGRYLLALIHETGAPIVASGCAMKALVESIARDWPARSAASPIPRPS
                     "
     gene            6522..7658
                     /locus_tag="PLP50_14325"
     CDS             6522..7658
                     /locus_tag="PLP50_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA52768.1"
                     /translation="MVALGTACSKPAAGPPPGPAEVGVVTMTPERVVLTTELPGRTNP
                     YLVAEVRPQVNGILRERTFEEGSDVKAGDLLYQIDPAPYQAAFEQAKASLAMAEANLP
                     AARSRAERMKGLVEIHAVGQQDYDDAVAALQQAEASVAAAKATVESARINLSYTPIRA
                     PISGRTGRSSVTVGALVTAYQPVPLATVQQLDPIYVDVAQSSADLLRLQKSLNGGALT
                     RDASWSRVKLILEDGTPYSREGKLKFREVTVDPTTGSVTLRMVFPNPDHVLLPGMYVR
                     AVVEEGVLSDGLLVPQQGVARTPKGEPYALVAGKDGKVEQRMLTVGRAMGDRWLVTAG
                     LAEGDQVIVEGTQRVRPGADVKAVPFVPAAPGAAATGPAAPAAK"
     gene            7668..>9435
                     /locus_tag="PLP50_14330"
     CDS             7668..>9435
                     /locus_tag="PLP50_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA52769.1"
                     /translation="MSSFFLKRPVFAWVIAIAMMLGGVLAIVKLPVSQYPPIAPPSIT
                     IRAQYTGASAKTVEDSVIQIIESKMTGLDSMLYISSVADSSGGAEVTLTFTPETDPDL
                     AWSKVQNKLQLALPALPDSVQKQGVTVSKSTRNFLLIVGLLSEDGSMTRADLADYVTS
                     QVESPLARVPGVGEVQVFGTGYSMRLWIDPDKLTKFGMTIDDVVAAVRAYNVEVSAGQ
                     FGGAPAVPGQRLNASILIQSLLVTPEEFAAIPLRHDADGSVVRVSDVGRTELGTEIPD
                     IEISYGSLPATAFAIRQEAGANALDTVARIKAKMEELSQFFPPGMKVVYPFDTTPFVR
                     VAIDEVVRTLIEAVILVFLVMYLFLGNLRATIIPTIAVPVVILGTFGVLALFGYSINM
                     LTMFAMVLAIGLLVDDAIVVVENVERVMSEEGLPPYEATKKSMEEISGALVGIGLVLA
                     AVFGPMIFFPGSTGVIYRQFSVTVIASMLLSVVVALILTPVLCASLLKPVAKGHEAAE
                     TGFRLLRPFFLWFDRTFYKGRDFYLAVVRHVLGRRARYLFVFLVIVGLMGLLYLRMPT
                     GYLPEEDQGSIMTMVQLPSGSTL"
BASE COUNT         1365 a         3371 c         3339 g         1360 t
ORIGIN      
        1 agctcctcgt cccggtcgag cggctcgacc tcctccagaa gtaccagagc ggcggcgagg
       61 ggccggcccc gcccctcgac aagctcggag gcgcgggctg ggcgaagcgg aaggcgtccg
      121 tccggaaggc ggtcaaggac atcgccgagc agctcctgaa gctctacgcc cgccgcgcca
      181 cgacgccggg ccattcgttc tcgaaggact cgccgtggca gaaggagttc gaggaggcct
      241 tcgagttcac ggagacgccc gaccagctct cggcgatccg ggacatcaag cgcgacatgg
      301 agtcggagag gtcgatggac cggctcctct gcggcgacgt cggctacggc aagaccgagg
      361 tcgcgatgcg ggccgtcttc aagtgcgtcc tggacggcaa gcaggccgcg gtcctcgcgc
      421 cgacgacgat cctcgccgac cagcaccacc ggaccttccg tcgacgcttc gcggccttcc
      481 ccgtgacgat cgacctcctc tcgcgcttcc ggacctccga cgagcagaag gaggtcgtgc
      541 ggcggctcgc ggaggggacg ctcgacgtcg tcgtcgggac gcaccggatc ctctcgaagg
      601 acgtctcctt ccgggatctc ggcctcgtcg tcatcgacga ggagcagcgc ttcggcgtcg
      661 cccagaagga gaagctgaag gagctgcgcg cgtccgtcga ggtcctctcg atgtcggcga
      721 cgccgatccc ccgatcgctg aacctctcgc tcggcgggat ccgcgacctg tcggtcatcg
      781 agacgccgcc gaaggaccgc ctcgcgatct cgacgcacgt cgtcccgcag ggggacgagg
      841 tcgtccgcga ggcgatccgc gctgagctcg accgcggcgg gcaggtctac ctcgtccaca
      901 accggatcga ggggctcggg gtctggcgcg agcgcctcgc ggagctcgtc ccggaatgcc
      961 gcgtcgtcgt tggccacggg cagatgtcgg agggggagct ggagcgggcg atgcgcgcgt
     1021 tcacgacgcg ggcggccgac ctcctcctcg cgacgacgat cgtcgagaac ggcctcgaca
     1081 tcccgaccgc gaacacgatg atcatcgacc gggccgacct ctacggtctc tcgcagctct
     1141 accagctgcg cggccgggtc ggccggagcg acaagccggc ctcctgctgg ctcctcgtcc
     1201 cgcccggcgc gccgctcagc gacgacgcgc gccggaggct gcgggcgatc caggagttct
     1261 cggacctcgg cgccggcttc cggatcgcgg cgaaggacct cgagatccgc ggctcgggca
     1321 acgtcctcgg cggcgagcag tcggggcaca tcgacgccgt cggcttcgag acctacgtca
     1381 ccctcctgga ggaggcgatc ggcgagctga agggagagcc ggtcgccgag aagcgggagg
     1441 tcacgctcca gctcggcctg cccctcggac tcccgaagaa gtggatcgga gaggagagcc
     1501 ttcggatggc gctctacaag cggctcgcga cggccgaagg ggtcgacgcg ctcgacgtcc
     1561 tggagaagga ggcgacggac cgctacggcg cgccgccgcc cgagttcgcg cggctcctcg
     1621 gcctcgggcg gctgcggctc ctcgcgctcg acctcggcgt ccgctcgctc cagcgccgcg
     1681 gcgccgagct cgccgtgacg ctcgacaagg ggcaccgcct cgaccccgac cgcctcctcg
     1741 gggcgatccg ggcgaagggg ctcgcggcct cggggccgga gtcgttccgc gcgccggagc
     1801 ttttccgggg gctcccgccc gacacggagg aggtctgcgg cgtcgcgggg cggttcctcg
     1861 tctccctcgc gcgccccgcg acggcgtttg cgccgggcct gctcgcggcg ttctcggaag
     1921 ggccccggtg aggaggggcg ccctggtcct cgtccctctg gtggcggccc tcgccctttc
     1981 cggatgcgct cgaaaggggc cccgggccgc cgcgcggacc tcggtcgtcg cgatggtcgg
     2041 cgacgagccg gtggaggagg aggacttcga gaactacgtc cgcgccgccg caggcgccgg
     2101 cctcggccag gtctcccccc aggtcgcctc gagcctcctc gaccagtacc tcgaggaacg
     2161 cctcctcgag cgggccgtcg ccgaggcgga gccctcgccg cgcgggaaga cggcggccga
     2221 gagacgggcc gaggtcctcg cccgggcggc gcgcctcgag cagatcaccg acaccgagct
     2281 gcgcgccgag tacgacgcgg agcccgcgcg ctgggagcac ccgccgctcg tgaggctctc
     2341 gcagatgatc cttcccgaca gggccgccgc cgagacggcg cgccggcggg tccttgccgg
     2401 agaggcgtgg gagaaggtct cccgggaggc gagccgcgcc ccgaacgcgg cgtcgggcgg
     2461 agggctcggc tggctctcgc gcaccgacct gccgagcgag ttcgagaagg cgatctgggg
     2521 gctgccggcc ggaggggtga gcccgcccca gccggccggt cacggggtcc atctgttccg
     2581 ggtggatgaa cgggcggacg ggcgggtcgt cccgttcgag gaggcgcggc cggcgctccg
     2641 gctggcgctg tccgagaagc ggagcagcga ggcgctggag cggctcctcg ccgaggcccg
     2701 caagcggcat cgcgtgggcg tcatcgagga gcacctcccg ttcccctacg tcgggggggc
     2761 ggcccgcttt tcgctagact ccgcgccgtg acgttccacc gacgccgtcc cggcaccgcc
     2821 gtcctcgccc tcctcgccgc cctcgcgctc cccgcgggcg ccgaggtcct cgaacagatc
     2881 ctcgtccacg tgaacgggcg gatcgtcacg cagagccagc tcgacgaccg ggtgcgggcc
     2941 gtcctcgccg aggccccgcc cgacctcgac caggggcgcc gcgacgacct ccggcgagcg
     3001 gcgctcaagg agctcgtcga cgaatcgctc ctcgaggacc gggcacggga cctggacctc
     3061 gtggcgacgg acgccgaggt cgaggagcag gtgaagcacc tgaaggaaca gaacaacctc
     3121 gcctcggagg aggagttcgt ccgcgcgctg gccgcgtcga acctgactcc ggcaaagctc
     3181 cgcgagcagc tgaaacggac ctcgacgatc cagcgcgtcg tcggccggga ggtcaacagc
     3241 cgcgtcgacc tctccgacga cgccctccgc ctcgtctacg agaggcagaa ggagacgtgg
     3301 gccgtccccg agaaggtccg cgtctcggag atcctcgtca atgcggggcc cggggcggag
     3361 gagaaggccc gcgaggccgc gtccctcgtg aaggggggcg cgcggttcga ggacgtcgtg
     3421 gtccgcttct ccgacggacc cacgcgctcc aagggtggcg acatggggac gttcggccgc
     3481 ggtgagctcg ccgcggcgct cgacgccgcc gcgttctcgc ttcccgccgg cggcgtgtcg
     3541 gacccgatcg agacgaagtc gggatggcac gtcctgaagg tcgtcgagaa gatccccgcc
     3601 ggctatcgtc ccttcgccga ggcgaagggg gagatcctgc ggaaggagca ggaaacgcag
     3661 ttccagaaga agctcgccga gtacgtcgag aagctgaaag gcgaggcgat catcaagtac
     3721 tcggacgaag cccgggccct gatgccccag ccctgacgtc gaggcggcgg agcctcgcgc
     3781 cgcgaggcag gcgtccggat atgctggctc cgcggaacgc gacgcgccga tgagcgacga
     3841 cccggtcccg acggtctccc gggaggagcg ctaccgggcg ctcctcggga tctcgaacgt
     3901 cctgggcgtc atccgcgacc ccgcggagct ggtcgggcgc ctcgcgcgcg agctccggca
     3961 gatcgccacg ttcgacttca tgagcctcta cctgggcgac gttcccgggc gaggcccgac
     4021 ctggaaggtc tggcgggtcg acgagcaggc gatcgtcgcg gtgccggagt cgatcccgct
     4081 cgaagagacc gtcgaggcct gggtcgccgc gaaccggcgt gtcgtggcct ggccccctcc
     4141 tgcggggcag ccctcgtttc cgaggctcga ggcggtgctc gagcggcacg gcatccgctc
     4201 ctacctcgcc gtcccgcttc tctcggcggg ccgcccggtc ggcgcgttga ccttcgggag
     4261 cttccgtccg ttggagtacg acgaggccga gacggagttc ctgtccgtcg tcgccgacca
     4321 agtcgcgctg gccatcggag gagcggccag cttcgagcgg gtccgggtcg cccacgagag
     4381 gctcgagcgg agcagcgagc ggctccggct cgtcctcgag gtgggcagcg ccgtcctcgc
     4441 gaacctcgac ctggaagagc tcctccgcgc gatctccgcg aacgtccgaa gggtcctccg
     4501 gtgcgacgtc gcgagcgtca tcctgctcga cgcggaaagg agccgggcgc ggctccacgt
     4561 cctcgacttc cccgaggggc gcgggttcgt gaaggaaggg gccgagatcc cggtccgccg
     4621 cgacgcggtc gaggagcttc tccgttcgag ccgaccgcgg gagctcgcgg tcgaccgcga
     4681 ggggacgccg ggctcggacg tggcgcgggc cgaggggatc cggcacggct ggaccttccc
     4741 gctcgtctcg cgaggcaggg ctctcggctt cttcacggtg ggtcggcggg acgaaacccg
     4801 gttcgacgcc gacgaggtgg acctcctcgc ccaggtcgcg gggcaggtgg ccaccgccct
     4861 cgacaacgcc ctcgcctacc gcgagatcgc cgggctgaag gaccagctcg cgcgggagaa
     4921 gctctacctc gaggacgaga tccgcagcga gatgagcttc gacgagatcg tcggccggag
     4981 cgaggcgctc cgaagggtgc tccggcaggt cgagacggtc gctccgaccg actcgacggt
     5041 cctgatccac ggcgagacgg ggtcgggcaa ggagctcgtc gcgcgcgcca tccacgaccg
     5101 gagcgcccgg gcgggccgga cgttcgtgaa gctcgactgc gccgcgatcc cgtcgggcct
     5161 cctcgagagc gagctcttcg gacaccagaa gggggcgtac acaggggcga tctccgaccg
     5221 cgtcgggcgc ttcgagctcg cgagcggcgg aaccgtcttt ctcgacgaga tcggcgaggt
     5281 gccgctggag cttcagccga agctcctccg gatcctccag gagagggagt tcgagcggct
     5341 cggcagctcg aagaccctcc gcaccgacgc tcgcctcatc gccgccacga accgcgacct
     5401 cgaggcgatg gtcgaagagg ggacgtttcg cgccgatctc ttctatcggc tgaacgtctt
     5461 cccgatccac gtccctccgc ttcgcgagag gccggaggac gtccccctcc tcgtccgcca
     5521 cttcgtccag cagctcggcc ggaagacgcg gaagaaggtc gagacgatcc cctccgagac
     5581 aatggcggcc ctccagcggt acgactggcc cgggaacatc cgggagctcc agaacttgat
     5641 cgagcgggcg ctcatcctga cgaccggccc tgtcctccag gtcccgctgg ttggactgaa
     5701 gtcgcgctcc ggcgcgcctc cggcgatccc gacccggacc cttcgagaag cggagcggga
     5761 gcacgtcctc gccgttctcg aaagcacggg gtgggtgctc gggggggcga acggcgccgc
     5821 cgcacgcctc ggcctcaacc gctcgacgct ccagttccgg ctgaagaagc tcgggctcgt
     5881 ccgaccagat cgcgggggcg ccgcggaccg cgcctgacgt cctgagtgcc aacatgttgg
     5941 cgcggtgcca atattttggc gccaatcttc tggcgttcgc ctgaaacccg aacgcggccc
     6001 gatccgttta gccgagtggg ttcaggggct tggagtgaga ccttcggcca gtctcccggg
     6061 gcatcggggt tgcgatagcg gactcggagg aagccgccga tgctcaggat caccatcgag
     6121 ccctacgagc aggaggttcg actgaagctc gagggggacc tcatcgggac atgggtccgc
     6181 gacctcgagg agagctggcg ggctgctcgg gagggcgggc gggacgccgg cctcgacctg
     6241 accggcgtca cgaaggtgga tgacgccggg cgttacctcc tcgccctgat ccacgagacc
     6301 ggcgccccga tcgtcgcgag cggctgcgcg atgaaggctc tcgtcgagtc gatcgcccgc
     6361 gactggccgg cccggtcggc cgcgtcgccg atcccccggc cgagctgagc cgctccggtc
     6421 ccgcgccgcg gatgcctttc gacacgcttc aagagatgga gatgagcatg aagacccgtt
     6481 ccttccccgc ttccgccctt cccctcggcg ccgtcctcgc gatggtggcc ctcggcacgg
     6541 cctgcagcaa gccggccgcg ggccctccgc cgggacccgc cgaggtcggc gtcgtcacga
     6601 tgacccccga gagggtcgtc ctgacgacgg agctcccggg ccggacgaac ccgtacctcg
     6661 tcgccgaggt ccgcccgcag gtgaacggca tcctgcgcga gcggacgttc gaggagggct
     6721 ccgacgtgaa ggcgggagac ctcctctacc agatcgaccc ggccccgtac caggcggcct
     6781 tcgagcaggc gaaggcgtcc ctcgcgatgg cggaggcgaa cctcccggcc gcgcgctcgc
     6841 gcgccgagcg gatgaagggc ctcgtcgaga tccacgccgt cggccagcag gactacgacg
     6901 acgcggtcgc ggcgctccag caggcggagg cgagcgtcgc cgccgcgaag gcgacggtcg
     6961 agagcgcccg gatcaacctc tcctacacgc cgatccgcgc gccgatctcg gggcggacgg
     7021 gacggtcgag cgtgaccgtc ggcgccctgg tgacggccta ccagcccgtc ccgctcgcga
     7081 ccgtccagca gctcgacccg atctacgtcg acgtggcgca gtcctcggcc gatctcctgc
     7141 ggcttcagaa gagcctcaac ggcggggccc tgacccgcga cgcctcctgg agccgcgtga
     7201 agctgatcct ggaggacggg accccctact cgcgtgaggg gaagctgaag tttcgcgagg
     7261 tgacggtcga cccgaccacc ggctccgtga cgctccggat ggtcttcccg aaccccgacc
     7321 acgtcctcct ccccggcatg tacgtccgcg ccgtcgtcga ggagggcgtc ctctccgacg
     7381 ggctcctcgt cccccagcag ggggtcgccc ggacgccgaa gggcgagccg tacgccctcg
     7441 tcgccggcaa ggacgggaag gtcgagcagc ggatgctgac ggtcggccgc gcgatgggcg
     7501 atcgctggct cgtgaccgcg ggcctcgccg agggggatca ggtcatcgtc gaggggaccc
     7561 agcgggtccg tcccggcgcg gacgtgaagg ccgttccctt cgtcccggcc gcgccgggcg
     7621 cggcggccac cgggccggcc gccccggcgg cgaagtaagg ggccgccatg tcgtccttct
     7681 tcctgaagcg cccggtcttc gcctgggtca tcgcgatcgc gatgatgctc ggcggggtcc
     7741 tggcgatcgt gaagctcccg gtctcccagt acccgccgat cgccccgccg tcgatcacga
     7801 tccgggcgca gtacaccggc gcgtcggcga agacggtcga ggacagcgtc atccagatca
     7861 tcgagtcgaa gatgaccggc ctcgactcga tgctctacat cagctccgtc gccgactcct
     7921 ccggcggcgc ggaggtgacc ctcaccttca cgccggagac ggacccggac ctcgcctggt
     7981 cgaaggtcca gaacaagctc cagctcgcgc tcccggccct gcccgactcg gtccagaagc
     8041 agggcgtcac cgtctcgaag tcgacgcgca acttcctcct catcgtcggg ctcctctccg
     8101 aggacggcag catgacgcgc gccgacctgg ccgactacgt cacctcgcag gtggagtctc
     8161 cgctcgcgcg cgtgccgggc gtcggcgagg tccaggtctt cggcaccggc tactcgatgc
     8221 gcctctggat cgacccggac aagctgacga agttcgggat gacgatcgac gacgtcgtcg
     8281 cggccgtgcg cgcctacaac gtcgaggtct ccgccggcca gttcggcggg gcgccggccg
     8341 tcccggggca gcggctgaac gcctcgatcc tgatccagtc gctcctcgtc acgccggagg
     8401 agttcgcggc gatcccgctc cggcacgacg cggacggctc ggtcgtgcgc gtgagcgacg
     8461 tcggccggac cgagctcggg accgagatcc cggacatcga gatctcctac ggcagcctgc
     8521 cggcgacggc cttcgcgatc cgccaggagg cgggggcgaa cgcgctcgac accgtcgcga
     8581 ggatcaaggc gaagatggag gagctctcgc agttcttccc gccggggatg aaggtcgtct
     8641 acccgttcga cacgaccccc ttcgtgaggg tcgcgatcga cgaggtcgtg aggaccctga
     8701 tcgaggcggt catcctcgtc ttcctcgtca tgtacctttt cctcgggaac ctccgggcga
     8761 cgatcatccc gacgatcgcc gtcccggtcg tcatcctcgg cacgttcggc gtcctcgccc
     8821 tgttcggcta ctcgatcaac atgctgacga tgttcgccat ggtcctggcg atcgggctcc
     8881 tcgtcgacga cgcgatcgtc gtcgtcgaga acgtcgagcg ggtcatgagc gaggaggggc
     8941 tcccgcccta cgaggcgacg aagaagtcga tggaggagat ctcaggcgcg ctcgtcggga
     9001 tcggcctcgt cctggccgcc gtcttcgggc cgatgatctt cttccccggc tcgaccggcg
     9061 tcatctaccg gcagttctcc gtcaccgtca tcgcctcgat gctcctctcg gtcgtcgtgg
     9121 cgttgatcct caccccggtc ctctgcgcct cgctgctgaa gccggtcgcg aaggggcacg
     9181 aggcggccga gaccgggttc cgcctcctcc ggccgttctt cctctggttc gaccggacgt
     9241 tctacaaggg ccgcgacttc tacctggccg tcgtccggca cgtcctcggg aggcgggcgc
     9301 gctatctctt cgtcttcctc gtcatcgtcg ggctgatggg gctcctctac ctgaggatgc
     9361 cgacggggta cctgccggaa gaggaccagg gctcgatcat gacgatggtc cagctcccct
     9421 ccggctcgac gctcg
//
LOCUS       DAOONL010000130         9068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_8958_length_9068_cov_12.875735, whole genome shotgun sequence.
ACCESSION   DAOONL010000130 DAOONL010000000
VERSION     DAOONL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8958_length_9068_cov_12.875735"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..608
                     /locus_tag="PLP50_14335"
     CDS             156..608
                     /locus_tag="PLP50_14335"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPA52770.1"
                     /translation="MADLIDGNNLLGQKGVSRETLVKELAELSRAKRKRLTVVFDGPP
                     EHGRPKVQQLGDVTVVYAAPRSADEEIVRRVREARDPRGVTVVTDDRVLSEAVDAAGG
                     RTSSVAAFRQTGARRMTAKAARDEEKRDPGGSASDWARWFSDPRNRIG"
     gene            605..1540
                     /locus_tag="PLP50_14340"
     CDS             605..1540
                     /locus_tag="PLP50_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPA52771.1"
                     /translation="MNVPLRRPRRLRPGSLVGVAALSGPVRPESLDAGVAALEGFGYS
                     VRVARNVLEREPLLGLAGDDDSRASGYLALVEDPEVEAILFARGGYGVTRLLQRLPAR
                     LLAETAKLHCGFSDLTALSAFLRERCGLVSIHGPMVAAELAAPLDSWTAPFFPALLEG
                     RGPARLTVPASELLVPGRAEGPLVGGCLSLLAALSGTPWDYDYEGALLFLEEVGEEAY
                     RVDRMLGTLVSSGRWTRLAGIIVGSMTRVTFGGSEDPERLRQLLFDRLAPLGVPVALG
                     LPFGHGGANVPLPVGARASWDGEARTLAFEEEFLS"
     gene            1537..2319
                     /locus_tag="PLP50_14345"
     CDS             1537..2319
                     /locus_tag="PLP50_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA52772.1"
                     /translation="MKVVLKSGGGAASFEEYIVRVPAGETVFSEGDVGTEMFVIQSGT
                     VDIVKKVKDEARVLSVLERGDFFGEMSILEDVPRTADAIARTDVELVRINQSTFDEML
                     RHNGEIAVRMLRKLSRRLRETTALLEQTTGEAPALDESSDFFERTGARDGIFRLVADG
                     GVEEFYLNRDTETLVGRIDPVTGIRPDVDLTNLDGPRSVSRRHAKIVRVDGEFQVIEE
                     IGTMNGTFVNGNRIATGAPVPIHDGDRLRFGLVDLTFRVTRS"
     gene            2322..4430
                     /gene="recG"
                     /locus_tag="PLP50_14350"
     CDS             2322..4430
                     /gene="recG"
                     /locus_tag="PLP50_14350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPA52773.1"
                     /translation="MPRPARGREPVGPGTALVGLPGIGPVRARALADAGLATVLDLLL
                     ELPVRHEDRARFARVADLVAGGPPLTLFGTITATRLVRTRRRGFSIYEATLEDESGSV
                     GLVFYSQPWLARWLTPGARAFVHGRAVEKRRLVLESPHVEAEPEEPGDAALSVGRVVP
                     IYRPQPGLPPRLRRRLVARALEEAAPKLPDRLRARDADGLGLPSLLDALREAHFPGSA
                     GGPGDPSLFRDRSSRHLRRLAFEELLELQVALARRRRERAARPAPRIEADSATRRSLA
                     AMLPFPLTAAQKQAIREIGADLRSGHPMARLLQGDVGSGKTIVAVLSAVLAARRGFQT
                     AFMAPTTILAEQHAETVHRLLAGTGVTAALLTARVTGRPRAALLEALAEGEIGLLVGT
                     HALLEKPVAFRRLGLVVVDEQHRFGVAQRAALPARASARHEHPHVLVLSATPIPRSLA
                     LALHGDLDVSTLDEKPPGRTPVVTSVLDEDGREEAYRRLAADVGAGGRAYVVVPLVEE
                     SETIEARSVASWTEEVARRLPGRRVGALHGKMRAEARETAMRRFAAGEIDVLVATTVV
                     EVGIDVPEASFLLVENAERFGLAQLHQLRGRVGRGSRASTCILLSGSGASPDALARLT
                     VLARTDDGFAVAEEDLRRRGPGEALGTRQSGLPDLRIADPLADGDLLGEAKELAVRLA
                     ADGATAPFEERLILRSSERS"
     assembly_gap    4592..4691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4692..5002
                     /locus_tag="PLP50_14355"
     CDS             <4692..5002
                     /locus_tag="PLP50_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52774.1"
                     /translation="TEASTITEYLRVARENGALRCVVDLSACTALPTTIVAVLMREQG
                     RFATSGGTLALAGVGEQNPFLTQSVAAGRFGHYRTVEEGLAAERRASGAPFDAPSGGT
                     "
     gene            5030..5704
                     /locus_tag="PLP50_14360"
     CDS             5030..5704
                     /locus_tag="PLP50_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPA52775.1"
                     /translation="MPDAPVSPEKVLEIVIQVAALLFAVSVHESAHGWMALRCGDTTA
                     RDLGRITLNPLKHIDPFGSLLVPALLAFTGAPVFGWAKPVPVSLRGVRNPRRANLLVS
                     AAGPLSNFAVAILAAVSFFILQAVAPAAPGHYESFFRPLLLVALFSVAVNVSLGIFNL
                     VPIPPLDGFGVVESFAPARWIRGVMWVRRYGFVFLVVAIFTGALSAVLRPPMTLVFQL
                     LLGGPS"
     gene            5701..6696
                     /gene="trpS"
                     /locus_tag="PLP50_14365"
     CDS             5701..6696
                     /gene="trpS"
                     /locus_tag="PLP50_14365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPA52776.1"
                     /translation="MSEPEKKIVLSGLRPTGRVHLGNYWGAVKNWVDLQDRYACRYFV
                     ADLHALTTDYADTSAIREATLEAVTDWLSVGLDPEKAVVFVQSQVPQTAELALVFGMF
                     TPLGWLERVPTYKEQLQELREKDLGTYGFLGYPVLMTADIALYRGHYVPVGKDQESHL
                     EICREIVRRFNGMYGEVFPEPQALFTETPKVPGLDGRKMSKSYDNTIALSDTAEAVTK
                     KVMSMVTDPRRARRTDPGNPDECNLFPFHKLYSSKEECAEIDRGCRTATLGCVDCKKW
                     LIRNMNAALEPVRAKRAEITAKPGFVREVLEEGGAKARALATDTMKSVSGAMKLL"
     gene            6772..7578
                     /locus_tag="PLP50_14370"
     CDS             6772..7578
                     /locus_tag="PLP50_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPA52777.1"
                     /translation="MAEGIRIPIPPGAELVQPYPVHLPQFEGPLDLLLHLIRKDEIDL
                     RNIPVADICRQYHEYLVLMQELDLEVAAEFLYVEALLVQIKSQMVLPRTPSTESGDAP
                     DPREELVNRLLQYRKFKGVAETLHVYEAERIGMYPRPAYRPEAEPEEEETDLSEVSLF
                     DLLTLFKGAVDRFRALHPPAMAIAAQKFSIREKMEDMAGRVRETGRVALTEVFRGLTG
                     RAEAIAVFLAVLELLRLLLVRALQTEEFGEIYLEPTGERLTLDGYEEEYR"
     gene            7583..8494
                     /gene="scpB"
                     /locus_tag="PLP50_14375"
     CDS             7583..8494
                     /gene="scpB"
                     /locus_tag="PLP50_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPA52778.1"
                     /translation="MTDDRDELNPGPLAPEAGLQTPPDAEPMAAEAPEDQSPSAAPPE
                     PANAELEEALPLVEALLFASSKPIAVEKLAEAAELSEELVARALEALEAACGASGRGV
                     RLDRVAGGVRLVSRPEYDYPVRRLLGLDGKTKLSMAALETLAIVAYRQPVTGPEIAEL
                     RAVNSSSSIRTLLDRKLITTAGRKEVVGTPFLYKTTKEFLVHFGLKGLGDLPKPEELE
                     AIYGLEAPKADPAQVELFHVDEATGEVEEVEEVEPVVASPGEGEPSGPGGGLSAAEPA
                     PGEAEPVGDAGPAPGDETARGEERHDG"
     gene            8484..>9068
                     /locus_tag="PLP50_14380"
     CDS             8484..>9068
                     /locus_tag="PLP50_14380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPA52779.1"
                     /translation="MTVERLQKVLAAAGVASRRAAEEMILEGRVTVNGTVVRELGAKA
                     DPAVDHVKVDGKRIRPAAAHRYILMNKPRGVVVTRDDPQHRPTVYHLLGTRVSEPVVP
                     VGRLDFDSEGLLLLTNDGALAHRIAHPSGGCQKEYEVKVSGVPTPARIARLARGVILD
                     GTRTAPAKVELTETTPEKDGVGGNAWLRVVLGEGR"
BASE COUNT         1236 a         3237 c         3188 g         1307 t
ORIGIN      
        1 ggggggcgcc cggatggcaa gcccccccgg agccagtccg gcccgggtca cttactccga
       61 ggaggtcgcc tcccgcccgc atcccgctgg gccgggcagc gtccgccgcc atcccgggcc
      121 gtgccgcgga gcgcccgatg tcggagaatg gagtcgtggc cgacctgatc gacggcaaca
      181 acctcctcgg gcagaagggc gtctcgcgcg agacgctggt caaggagctg gcggagctct
      241 cccgcgcgaa gcgaaagcgg ctgactgtcg tcttcgacgg cccgccggag cacggccggc
      301 cgaaagtcca gcagctcggg gacgtgaccg tcgtctacgc ggcgcctcga agcgccgacg
      361 aggagatcgt ccgccgggtc cgggaggcgc gcgacccgcg cggcgtgacg gtcgtgacgg
      421 acgaccgggt cctgtcggag gcggtcgacg cggcgggcgg acgaacgtcc tccgttgccg
      481 cgttccggca gaccggtgcg cggcggatga cggcgaaggc cgcccgggac gaggagaaga
      541 gggaccccgg cgggagcgcc tccgactggg cccgctggtt cagcgacccg cggaacagga
      601 tcgggtgaac gttcccctgc ggcgtccccg ccggctccgc cccgggagcc tcgtcggcgt
      661 ggctgcgctc tcgggcccgg tccgcccgga gagcctggac gccggcgtcg cggctctcga
      721 gggcttcggc tacagcgtcc gggtcgcccg gaacgttctc gagagagagc cgcttctcgg
      781 cctggccggc gacgacgatt cccgcgcgag cggctatctc gccctcgtgg aggaccccga
      841 ggtcgaggcg atcctcttcg cgcggggcgg ctatggcgtg acgcgcctcc tgcagcgcct
      901 tccggcgcgc cttctcgcgg agacggcgaa gctgcattgc ggcttctccg acctgacggc
      961 gctttcggcg ttccttcgcg agcgctgcgg cctcgtctcg attcacggtc cgatggtcgc
     1021 ggccgagctg gcggctcccc tcgactcctg gaccgcgccg ttctttcccg ccctactgga
     1081 gggacgtggc ccggcccgcc tgaccgttcc cgcgtcggag ctcctcgtcc cggggcgggc
     1141 ggaggggccg ctcgtcgggg gctgcctctc tctcctcgcc gcgctgagcg ggacgccgtg
     1201 ggactacgac tacgagggag ccctgctctt cctggaggag gtgggggagg aagcctaccg
     1261 agtcgaccgg atgctcggga cgctcgtttc ttccggccgg tggacccggc tcgccggtat
     1321 catcgtgggc tccatgacac gcgtgacgtt cggcggcagc gaggacccgg agcgcctccg
     1381 gcagctcctc ttcgaccggc tcgccccgct cggggttccg gtcgccctcg gcctgccgtt
     1441 cgggcacggg ggggcgaacg tgccgctccc cgtcggagcg cgtgcttcgt gggacggcga
     1501 ggcgcggacg ctcgccttcg aggaggagtt cctctcgtga aagtcgttct gaagtccggc
     1561 ggaggcgccg cgagcttcga ggagtacatc gtacgggtcc ccgccggtga gaccgtcttc
     1621 tccgaaggag acgtcggcac ggagatgttc gtgatccagt ccgggacggt cgacatcgtg
     1681 aagaaggtga aggacgaggc gcgggtcctc tccgtcctcg agagaggcga cttcttcggc
     1741 gagatgtcga tcctcgagga cgtcccccgg acggcggacg ccatcgcgcg gaccgacgtc
     1801 gagctcgtcc ggatcaacca gtcgacgttc gacgagatgc tccggcacaa cggcgagatc
     1861 gccgttcgga tgctccggaa gctctcccgc cggctccggg agacgaccgc gctgctcgag
     1921 cagacgacgg gagaggctcc ggccctcgac gagtcgtccg acttcttcga gcggacgggc
     1981 gcgcgggacg ggatcttccg ccttgtcgcg gacggagggg tcgaggagtt ctacctgaac
     2041 cgcgacacgg agacgctggt cggacggatc gaccctgtga ccgggattcg gccggacgtc
     2101 gacctgacga acctggacgg accgcgctcg gtctcccgcc ggcacgcgaa gatcgtccgc
     2161 gtcgacgggg agttccaggt gatcgaggag atcgggacga tgaacggcac gttcgtgaac
     2221 ggaaaccgga tcgccaccgg cgccccggtg ccgatccacg acggcgaccg cctccgcttc
     2281 ggtctcgtcg acctgacctt ccgagtcacg cggagctgag cgtgccgcgc cccgcgcgcg
     2341 gccgggagcc cgtcggtccg ggaacggctc tcgtcgggct tcccgggatc ggcccggtcc
     2401 gggcgcgagc tctcgcggac gccggtctcg ccaccgtcct cgacctcctg ctcgagctgc
     2461 cggttcgcca cgaggaccgg gcccggttcg cccgggtggc ggacctggtc gccggaggac
     2521 cgccgctcac gctgttcggg acgatcaccg cgacccgcct ggtgcggacg cgccggcgcg
     2581 gtttctcgat ctacgaggcg acgctcgagg acgagagcgg ctccgtgggc ctcgtcttct
     2641 actctcagcc ctggctcgcg cgctggctga cgcccggcgc gcgagccttc gtgcacggcc
     2701 gcgcggtgga gaagcgacgg ctggtcctcg agtcgcccca cgtcgaagcc gagcccgagg
     2761 agcccggcga cgccgcgctc tccgtgggtc gcgtcgtgcc gatctacagg cctcagccgg
     2821 ggctcccgcc ccgcctccgg cggcggctgg tcgcgcgcgc cctcgaggag gcggctccga
     2881 agctgccaga ccggctgcgc gctcgggacg ccgacgggct cggccttccc tccctcctcg
     2941 acgcgctccg cgaggctcac ttcccgggga gcgccggagg gcccggcgac ccgtcgctct
     3001 ttcgggaccg cagctcccga cacctccgcc ggctcgcctt cgaggagctc ctggagctcc
     3061 aggtcgcgct ggcgaggcgg cgaagggaga gggccgcccg gcccgctccc cgcatcgagg
     3121 ccgacagcgc gacgcgccgt tcgctcgccg cgatgctccc gttcccgctg acggccgctc
     3181 agaagcaggc gattcgcgag atcggagccg acctgcgttc gggtcacccg atggcgcgcc
     3241 tccttcaggg cgacgtcgga agcggcaaga cgatcgtcgc cgttctgagc gccgtcctcg
     3301 cggcccggcg cggcttccag acggccttca tggcgccgac gacgattctg gccgagcagc
     3361 acgccgagac cgtccaccgg ctcctcgccg gcaccggcgt cacagcggcg ctgctgacgg
     3421 cccgcgtgac cggacgtccg cgggcggccc tgctcgaggc gctggccgag ggggagatcg
     3481 gtctcctcgt cgggacgcac gccctcctgg agaagcccgt tgcgttccgt cggctcggcc
     3541 tcgtcgtcgt cgacgagcag caccgtttcg gcgtggcgca gcgcgccgcg cttccggcgc
     3601 gggcctccgc gcgccacgaa cacccgcacg tcctcgtcct ctcggcgacg ccgatccccc
     3661 gctcgctcgc gctcgcgctc catggcgacc tcgacgtctc gacgctcgac gagaagcctc
     3721 cggggcgaac cccggtcgtg acgtccgtcc tggacgagga cgggcgcgag gaggcgtacc
     3781 ggcgtctcgc cgccgacgtc ggggcggggg ggcgcgccta cgtcgtcgtc cccctcgtcg
     3841 aggagtccga gacgatcgag gcgcgttccg tcgcctcctg gacggaggag gtcgcccggc
     3901 gcctccccgg ccggcgcgtc ggcgccctcc acgggaagat gcgggccgag gcgcgggaga
     3961 cggcgatgcg ccgcttcgcg gccggagaga tcgacgtcct ggtcgccacg acggtcgtcg
     4021 aggtcggcat cgacgtcccc gaggcctcgt tcctcctcgt cgagaacgcg gaacgtttcg
     4081 gcctcgcgca gctccaccag cttcgcggcc gcgtggggcg cgggagccgg gcttcgacct
     4141 gcatcctcct ttcgggatcc ggggcttcgc ccgacgccct cgcgcgcctc acggtcctcg
     4201 cgcggaccga cgacggattc gcggtggccg aggaggacct tcgccgccgc ggcccggggg
     4261 aggccctcgg cacgcgccag agcgggctcc ccgaccttcg gatcgccgat ccgctcgccg
     4321 acggggacct cctcggggag gcgaaggagc tggccgtccg gctcgccgcg gacggcgcga
     4381 cggctccttt cgaggagcgg ctgatcctcc ggtccagcga gcggtcctga gggagcggat
     4441 ccgtccgcgt agcgctgtgg catcctccgg gtgtggagct ttccctcgag accgacggat
     4501 cggtcgccgc gctgaaggtc cggggcgacg tgacgctcac ggaggcgtcg acgatcacgg
     4561 agtacctccg cgtcgcccgc gagaacggcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn ntcacggagg cgtcgacgat cacggagtac ctccgcgtcg cccgcgagaa
     4741 cggcgcgctg cgctgcgtgg tcgacctgtc cgcctgcacg gccctcccga cgacgatcgt
     4801 cgccgtcctc atgcgcgagc aggggcgctt cgccacctcg ggcgggacgc tcgctctggc
     4861 gggagtgggg gagcagaacc cgttcctcac gcagagcgtc gccgccggcc gcttcggcca
     4921 ctatcgaacg gtcgaggagg ggctcgccgc cgagcggcgc gcgtccggcg caccgtttga
     4981 cgccccctcg gggggcacgt aggatcgggc gttttcggac gttcctgccg tgcccgacgc
     5041 gcccgtctcc cccgaaaagg tcctcgagat cgtcatccag gtggccgccc tgctgttcgc
     5101 ggtctccgtg cacgaatccg cgcacggatg gatggccctg cgctgcggcg acacgacggc
     5161 ccgggacctc gggcggatca cgctgaaccc cctgaagcac atcgacccgt tcggaagcct
     5221 gctcgtcccc gcgctcctcg cgttcaccgg agcgccggtc ttcgggtggg cgaagccggt
     5281 gccggtctcg ctccgcggcg tccggaaccc gcgacgggcg aacctcctcg tctcggccgc
     5341 cgggccgctc tcgaacttcg ccgtggcgat cctcgcggcg gtctcgttct tcatcctcca
     5401 ggccgtcgcg ccggcggcgc caggccacta cgaatcgttc ttccgcccgc ttctcctcgt
     5461 ggccctcttc tcggtggcgg tgaacgtttc gctcggcatc ttcaacctcg tcccgatccc
     5521 gcctctcgac ggcttcggcg tcgtcgagag cttcgccccg gcccgctgga tacgcggcgt
     5581 catgtgggtg cggcgatacg ggttcgtttt cctcgtcgtg gcgatcttca cgggggcgct
     5641 ctcggccgtc ctgcggccgc cgatgacgct cgtcttccag ctccttctcg gaggtccctc
     5701 ttgagcgaac cggaaaagaa gatcgtcctc tcgggcctca ggcccacggg acgcgtccac
     5761 ctcggcaact actggggcgc ggtgaagaac tgggtcgacc tgcaggaccg gtacgcgtgc
     5821 cggtacttcg tggccgacct ccacgccctg acgaccgact acgccgacac gtcggcgatc
     5881 cgcgaggcga ccctcgaggc cgtgaccgac tggctctcgg tggggctcga cccggagaag
     5941 gcggtcgtct tcgtccagtc gcaggtgccc cagacggcgg agctcgcgct cgtcttcgga
     6001 atgttcaccc cgctcggctg gctcgagaga gtcccgacct acaaagagca gctccaggag
     6061 ctgagggaga aggacctcgg cacctacggg ttcctcggct acccggtcct gatgacggcc
     6121 gacatcgccc tctatcgcgg ccactacgtc cccgtgggga aggaccagga gagccacctc
     6181 gagatctgcc gcgagatcgt gcggcgcttc aacgggatgt acggcgaggt cttccccgag
     6241 ccacaggcgc tcttcaccga gacgccgaag gtccccggcc tcgacggccg gaagatgtcg
     6301 aagagctacg acaacacgat cgcgctttcc gacacggccg aggccgtgac gaagaaggtg
     6361 atgtcgatgg tgaccgaccc gcggcgcgcg cgtcggaccg atccgggcaa cccggacgag
     6421 tgcaacctct tcccgttcca caagctctac tcctcgaagg aggagtgcgc cgagatcgac
     6481 cgcggctgcc ggacggcgac gctcggctgt gtcgactgca agaagtggct gatccggaac
     6541 atgaacgcgg ccctcgagcc ggtccgcgcg aagcgggcgg agatcaccgc gaaaccgggg
     6601 ttcgttcgcg aggtcctcga ggaggggggc gcgaaggccc gggcgctggc gactgacacg
     6661 atgaagtccg tctccggcgc gatgaagctg ctttgaggcc gtcgtcgtcc cccacctccc
     6721 cccgggccgt ccgcgcttcg ctcgcgacgg cggacggacg ctgaggcgcc cgtggccgaa
     6781 ggcatccgga tcccgattcc ccccggcgcc gagctggttc aaccctaccc ggtccacctg
     6841 ccccagttcg agggtccgct cgacctcctc ctccacctga tccggaagga cgagatcgac
     6901 ctccggaaca tcccggtcgc cgacatctgc cggcagtatc acgagtacct cgtcctgatg
     6961 caggagctgg acctggaggt cgccgcggag ttcctctacg tggaggcgct cctcgtccag
     7021 atcaagagcc agatggtcct gcctcggacc ccgtcgaccg agagcggcga cgcccccgat
     7081 ccgcgcgagg agctggtgaa ccggctcctg cagtaccgga agttcaaggg cgtcgccgag
     7141 acgctccacg tctacgaggc ggaacggatc gggatgtacc cgcggccggc gtaccggccc
     7201 gaggccgagc ccgaggagga ggagacggac ctgtccgagg tctctctctt cgacctcctc
     7261 acgctcttca agggggccgt cgaccgcttc cgggcgctcc acccgcctgc gatggcgatc
     7321 gcggcccaga agttctccat ccgggagaag atggaggaca tggcggggcg ggtccgcgag
     7381 accggacgtg tcgcgctgac ggaggtcttc cgggggctca cgggacgcgc ggaggcgatt
     7441 gcggtcttcc tcgccgtcct cgagctcctc cgcctcctcc tggttcgagc gctccagacg
     7501 gaggagttcg gagagatcta cctcgagccg acgggcgaaa ggctgacgct ggacggctac
     7561 gaagaggaat accgctgaga cgatgacgga cgaccgagac gaattgaacc cggggcccct
     7621 cgcgcccgag gccggcctcc agaccccgcc cgacgcggag ccgatggctg ccgaagctcc
     7681 ggaggatcag tcgccctccg ccgctccccc cgagcccgcc aacgcggagc tggaggaggc
     7741 ccttcccctc gtcgaggccc tcctcttcgc ctcctccaag ccgatcgccg tcgagaagct
     7801 ggccgaggcg gcggagctct ccgaggagct cgtcgcgcgg gcgctcgagg ccctcgaggc
     7861 ggcctgcgga gcctccggac gcggcgtccg gctcgaccgg gtcgccggag gcgtccggct
     7921 cgtgtcgcgc cccgagtacg attaccccgt ccgcaggctc ctcggcctcg acggaaagac
     7981 gaagctctcg atggcggcgc tcgagaccct cgcgatcgtg gcgtaccggc agccggtgac
     8041 cggtccggag atcgccgagc tgcgcgccgt gaactcctcc tcctcgatcc gcacgcttct
     8101 cgaccggaag ctgatcacga cggccgggag gaaggaggtc gtcgggaccc ccttcctcta
     8161 caagacgacg aaggagttcc tcgtccattt cggcctgaag ggactgggag accttccgaa
     8221 gccggaggag ctggaggcga tctacggcct cgaggcgccg aaggccgacc cggcgcaggt
     8281 ggagctgttc cacgtcgacg aggccaccgg cgaggtggaa gaggtcgaag aggtcgagcc
     8341 cgtcgtcgcg agccccgggg aaggggagcc gtccggcccg ggcggaggtc tttccgcggc
     8401 ggaacccgcg cccggcgaag cggagcccgt cggggatgcc ggacccgcgc ccggcgacga
     8461 gacggcacga ggagaggagc gtcatgacgg ttgaacggct tcagaaggtc ctggccgcag
     8521 ccggcgtcgc ttcccgccgc gcggccgagg agatgattct cgagggccgc gtcacggtga
     8581 acgggacggt ggtccgcgag ctcggcgcga aggccgaccc cgcggtcgac cacgtgaagg
     8641 tggacggaaa gagaatccgg cccgccgcgg cccatcgcta catcctgatg aacaagcccc
     8701 ggggcgtcgt cgtcacccgg gacgacccgc agcaccggcc cacggtctat cacctgctcg
     8761 ggacgcgcgt ctcggagccg gtcgtcccgg tggggaggct cgacttcgac tcggaggggc
     8821 tcctcctgct gacgaacgac ggggctctcg cgcaccggat cgcgcacccg tcgggcggct
     8881 gccagaagga atacgaggtg aaggtgtcgg gagtcccgac ccccgcgcgg atcgcgaggc
     8941 tcgcccgcgg ggtcatcctc gacggcacgc ggaccgcccc cgcgaaggtc gagctgaccg
     9001 agacgactcc cgagaaggac ggcgtcggcg ggaacgcctg gctccgcgtc gtcctcggcg
     9061 aggggcgc
//
LOCUS       DAOONL010000131         8838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9273_length_8838_cov_18.475919, whole genome shotgun sequence.
ACCESSION   DAOONL010000131 DAOONL010000000
VERSION     DAOONL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_8838_cov_18.475919"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..462)
                     /locus_tag="PLP50_14385"
     CDS             complement(172..462)
                     /locus_tag="PLP50_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HPA52780.1"
                     /translation="MAQAARLLDMHTCPMTNPDGSPHTGGPIQKAEGTVLIGYFPAAR
                     VGDSATCAGPMDMITTGAARCLIAYMPAARVGDQTTHGGVIVAGCTSVLIGD"
     gene            complement(474..2714)
                     /gene="tssI"
                     /locus_tag="PLP50_14390"
     CDS             complement(474..2714)
                     /gene="tssI"
                     /locus_tag="PLP50_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HPA52781.1"
                     /translation="MGSYVQQNRKIRVTTPIGEDVLLLKSFEGTDELSRPFRYRLEMI
                     AENKAKVPFDGVLGKKVTVHLQLPDESSEHHLNGVCTRFSQAGRDDTFTLYRAELVPE
                     FQLLSRRARSRIFQRMSVPEILKEVMKGVTADWKLQGTYEKRDYCVQYRETDFNFASR
                     LMEEEGIFYFFRQEDGAHTMVVADASSEFPELPGESELIYEELEGGTRDENRIWAWER
                     AQEARSVRCVLWDHCFELPHKHLEAEEPILASVSAGREAHPLAVEGSDEWELYDWPGE
                     YAQRFDGINRSGGEQQDELQKIFHDNARTAGLRMDEEAALAVEIRGSSNVKHLAAGHT
                     LTLKRHFSGDGAYVLASVTHRATFGADYRSGKDEELAYANEFTCFPSALPFRPRRRTP
                     KPVVPGSQTAVVVGPKGEELFTDRYGRVKVQFHWDRDGKHDEDASCWVRVAQLAAGRR
                     WGSSFWPRIGQEVIVDFLEGDPDRPIIVGTVYNADQMPPYLGQGPDGKHRNDNRVSGI
                     KTSTTTGGNGFNELRFDDAKGAEQLFVHAERDLDVRVKNDARESVLNDKHVTVGGKDD
                     GERKGSYLEKVFQDKHVAVLGTLQEKVGDAKLTVGGDEGGGNLDVYVTGSEKRTVGGH
                     AEIRISRCRSTFVGEAEMLEANGDLGILGHQRVAIRGDGAATYSSRERVLIAAPDVAI
                     GEGDNFIRITSSGVTIVGTMVMINSGGCRGEGTAFDPEPVGEADRAEPKVPTAADDAK
                     TGQKSS"
     gene            complement(2973..5624)
                     /gene="tssH"
                     /locus_tag="PLP50_14395"
     CDS             complement(2973..5624)
                     /gene="tssH"
                     /locus_tag="PLP50_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HPA52782.1"
                     /translation="MSVNLKSLIGRLNDTCRQALEGAAGLCLSRTNYDVEVEHLLLKL
                     LEAPDTDLAKVLRRFEIDASRFAKDVVRALDRLKTGNARTPALSPRIPRLIEKAWVVA
                     SIEYGASRVRSGHLVHVLLTDDDLSRLAREISKDLAGISVEQLTKTFPDVVKGSAEDR
                     EAQALSEAPGAAGGPDVAMGVPGKTKSLDQYTEDLTAKARAGKIDPILGRDFEIRQVI
                     DILTRRRQNNPILTGEAGVGKTAVVEGFAQRIAQGDVPGPLKNVAVRTLDLGLLQAGA
                     GIKGEFENRLKQVIDEVKASPTPIILFIDEAHTMIGAGGTAGQNDAANLLKPALARGE
                     LRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPSEEIAIVMMRALAKNLEKHHGGLR
                     ILDEAVESSVKLSHRYISGRQLPDKCVSVLDTACAKVNISQAATPAPIEDCRRRIDQL
                     TAEIDALEREAAAGAAHGERIEKAKAAKAETEARLAVLDEQCKKEIELVNQIKETRGK
                     LEAAVGGADVAGLEGLRGELDRLKAELKTVQGDTPMMQADVNAQAVAEVIAGWTGIPV
                     GKMQADEIETVRNLAKLLGERVVGQDHALAAIAQKIQTSRAGLTDPRKPIAVFMFVGT
                     SGVGKTETAIALAETLYGGERNMVTINMSEFQEAHTVSSLKGSPPGYVGYGEGGVLTE
                     AVRRKPYSVVLLDEVEKAHPDVLELFYQVFDKGQMEDGEGREIDFKNCVILLTTNAES
                     DAMMKMCADPDTAPTPEKIVETIKPGLDKVFKPAFLGRMSIVPYYPISEPVMKLIIKL
                     QLGRIGKRLMENHGATFTYDGAVIDEIASRCKEVESGARNVDAILTRTVLPDLSGEFL
                     ARMAQGESISKVHVRVGEGGAFAYDVA"
     gene            complement(5653..6648)
                     /gene="tssG"
                     /locus_tag="PLP50_14400"
     CDS             complement(5653..6648)
                     /gene="tssG"
                     /locus_tag="PLP50_14400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HPA52783.1"
                     /translation="MKDLLFSEPYRFSFFQAVRLLERTSPEKEGVGRAVRPGNEIVRF
                     RTHPSLAFPPSEIVDLKAPPPGQEDRPPEMTVNFIGLTGPLGVLPHPYTELVRERVSY
                     KDTALWSFLDLFHHRFTSLFYRAWEKYRFPIAFERSGEDSFTEYLFDLIGMGTPGLRG
                     RMTVRDQALLLYAGLIAQKPHSGEAIASVLRDYFRVPAGIVQFQGQWFPMEEENVTRL
                     GAANSELGRTLVAGSSVFVSQSKFRVRLGPLPLSALVSFLPVGPAFRPLTELVRFLAG
                     LEFDYDVQLVLRKEEVPPCSLDSNAALPPMLGWTTWITTDTPREDAGDVVLPVDN"
     gene            complement(6656..8512)
                     /gene="tssF"
                     /locus_tag="PLP50_14405"
     CDS             complement(6656..8512)
                     /gene="tssF"
                     /locus_tag="PLP50_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HPA52784.1"
                     /translation="MRDELLGYYERELSFLRQMGAEFAEKYPKIAGRLQLEPDTCEDP
                     HVERLIEAFAFLASRVHLKVDDEFPEITESLLNVLYPHLLAPIPSLSIVQFVLDPSQV
                     NLQSGQKIPRGSMLYSRSVAGTSCRFRTAYPVRIWPVEMRQARFELPIPGVGPEEGVR
                     TVLRLELKTFGGAPLRELKEKVSESEEKPLDSLRFFLQGEGKLVYALHELIHNHLVAV
                     ELRPGGPRDVPSPVRLPKTSVAGVGFGRDEGLLPYTDRSFLGYRLLAEYFAFPEKFFF
                     FDVNGLAGAARDDFDDVLEILLYFDRDFPLERNVTAQTFRLNCTPVANLFRQVAEPIR
                     LTHLQPEYRVIPDVRRQDVMEVYSVEAVTSVAKGGDKVTSYEPFYSYRHGFEKGQAPA
                     FWYMSRRPSARKDDDGTDAYLSLVDLGFDPAEVDADTLTVETLCTNRDLPSRLPFEGG
                     EADFQLEGAGVFSGIRCLRRPTPTVRPPLRRGLQWRLLSHLSLNLLSLVERADGQGPE
                     ALQEILRLYDFADSSVTRQQIAGITKVGARRVLRSIGVLQPSFVRGVEVTLELDENQF
                     VGTGIFLFAQVLERFLGLYSSVNSFSQLVLTSRQREGVVRRWEPRAGEQIVL"
     gene            complement(8512..>8838)
                     /gene="tssE"
                     /locus_tag="PLP50_14410"
     CDS             complement(8512..>8838)
                     /gene="tssE"
                     /locus_tag="PLP50_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HPA52785.1"
                     /translation="NTRATAVVPPELKEVWSSIVVYGLPDITALSGRSPADKAKLRRA
                     VERVLETFEPRLEGVVVTVDPEPGPGLSLKFRIDGRLKVDPAPEPVSFDTLLQIGSGE
                     FRVKGE"
BASE COUNT         1241 a         3072 c         3064 g         1461 t
ORIGIN      
        1 gtcgcggaga aggggctgat gccgctcctg acgatgaagg ggaccgacac cgtccgcctc
       61 gcgatcttcc agtcgatcgc ggagtcgccg gcctctctcg ccgggcgctg gagctgaccg
      121 ggccgcgcgg cgccgccgtc cgatcgccgc ggagccgtcg gtcctctgcg atcagtcccc
      181 gatgaggacc gacgtgcagc cggcgacgat caccccgccg tgcgtcgtct ggtcgcccac
      241 acgtgccgcc ggcatgtagg cgatcagaca cctcgcggcg cccgtggtga tcatgtccat
      301 gggtccggcg cacgtcgccg agtcgccgac gcgcgccgcg ggaaaatatc cgatcagcac
      361 ggtgccctcg gccttctgga tcggcccgcc ggtatgaggg ctcccgtccg ggttcgtcat
      421 cggacaggtg tgcatgtcga gcagccgcgc ggcctgcgcc atcgctcccc tcctcagctc
      481 gacttctggc cggtcttcgc gtcgtccgcg gccgtgggga ccttcggctc agccctgtcc
      541 gcttcgccga ccggttccgg atcgaaagcg gttccttccc ctcggcaccc accgctgttg
      601 atcatcacca tggtgccgac gatcgtgacc ccggacgagg tgatccggat gaagttgtcg
      661 ccctctccga tcgcgacgtc gggcgcggcg atgaggaccc gctcccggct cgagtacgtt
      721 gcggccccgt cgccccggat cgcgacgcgc tggtggccga ggattccgag gtcgccgttc
      781 gcctcgagca tctcggcctc cccgacgaag gtgctccggc acctcgagat ccggatctcc
      841 gcgtgccctc cgacggtccg cttctcgctt cccgtcacgt agacgtccag gttcccgccc
      901 ccctcgtctc ccccgacggt gagcttcgcg tcccccacct tctcctggag cgtcccgagg
      961 acggcgacgt gcttgtcctg gaaaaccttc tccaggtagc ttcccttcct ctccccgtcg
     1021 tccttgcccc ccaccgtgac gtgcttgtcg ttcaggacgc tctccctcgc gtcgttcttc
     1081 accctgacgt ccaggtcccg ctcggcgtgg acgaagagct gctccgcccc cttcgcgtcg
     1141 tcgaagcgga gctcgttgaa tccgttcccc cccgtcgtcg tgctcgtctt gatccccgac
     1201 acccggttgt cgttccggtg cttgccgtcg gggccctggc cgaggtaggg cggcatctgg
     1261 tcggcgttgt agacggtccc gacgatgatc ggccggtccg ggtcgccctc gaggaagtcg
     1321 acgatcacct cctggccgat ccgcggccag aaggaggatc cccatcgccg ccccgccgcg
     1381 agctgggcga cccgcaccca gcaggacgcg tcctcgtcgt gcttcccgtc ccggtcccag
     1441 tggaactgga ccttcacccg accgtacctg tcggtgaaaa gctcctcccc cttcggcccg
     1501 acgacgacgg ccgtctggct ccccggcacg accggcttcg gcgtccgccg cctcggccgg
     1561 aacgggagcg ccgacgggaa acaggtgaac tcgttcgcgt aggcgagctc ctcgtccttc
     1621 cccgaacggt agtccgcccc gaacgtcgcc cggtgcgtca ccgacgcgag gacgtacgcc
     1681 ccgtcgcccg agaagtgcct cttgagcgtc agcgtgtggc ccgccgcgag gtgcttgacg
     1741 ttgctcgagc cgcggatctc gacggcgagc gccgcctcct cgtccatccg gaggcccgcc
     1801 gtgcgcgcgt tgtcgtggaa gatcttctgc agctcgtcct gctgctcccc gcccgaccgg
     1861 ttgatcccgt cgaaacgctg cgcgtactcc cccggccagt cgtagagctc ccactcgtcg
     1921 gagccctcca ccgcgagcgg gtgcgcctcc cgcccggcgg agacggaggc gaggatcggc
     1981 tcctccgcct ccaggtgctt gtgcggcagc tcgaagcagt ggtcccagag gacgcaccgg
     2041 accgatcgtg cctcctgggc ccgctcccag gcccagatcc tgttctcgtc gcgggtgccc
     2101 ccttccagct cctcgtagat gagctcgctc tctcccggca gctcggggaa ctcggaggag
     2161 gcgtcggcca cgaccatcgt gtgcgcgccg tcctcctgcc ggaagaagta gaagatcccc
     2221 tcctcctcca tcaggcggga tgcgaagttg aagtcggtct cccggtactg gacacagtag
     2281 tcccgcttct cgtacgtccc ctggagcttc cagtcggcgg tgacgccctt catcacctcc
     2341 ttgaggatct ccggcaccga catccgctgg aagatccggc tccgcgccct ccgcgagagg
     2401 agctggaact ccggcacgag ctcggccctg tagagcgtga acgtgtcgtc gcggcccgcc
     2461 tgcgagaacc gcgtgcagac gccgttgagg tggtgctcgc tcgactcgtc cggcagctgg
     2521 aggtggacgg tcaccttctt gccgaggacg ccgtcgaagg ggaccttcgc cttgttctcg
     2581 gcgatcatct cgagccggta gcgaaacggc cgggagagct cgtccgtccc ctcgaacgac
     2641 ttcaggagga ggacgtcctc gccgatcggg gtcgtgacgc ggatcttgcg gttctgctgg
     2701 acgtagctgc ccacgcgata cccccgtttc ttgcccgccg cccccgtccc ggtcccgttc
     2761 tctccaatat ggtcccggat ccgctcgggc gcgacgcctt tcgggccgct cgggcgtgcc
     2821 ccgcgtcacg ccgggcgacg ttcgcgtgcc cgcacgagcg acggcttctc agcttccgcc
     2881 tgcccggccg cctcacgccc gtccctgcac ggacgaaggg ccgggaccgc ctgcgcggtc
     2941 ccggccctcc gacccgatcc ggcggggagg ggtcaggcga cgtcgtaggc gaaggcgccg
     3001 ccctccccca cgcggacgtg gaccttcgag atcgactccc cctgcgccat ccgcgcgagg
     3061 aactcgccgg agaggtcggg gaggacggtc ctcgtgagga tcgcgtcgac gttgcgggcg
     3121 ccgctctcga cctccttgca gcggctcgcg atctcgtcga tcaccgcgcc gtcgtacgtg
     3181 aaggtcgcgc cgtggttctc catgaggcgc ttcccgatcc ggccgagctg gagcttgatg
     3241 atcagcttca tcacgggctc ggagatcggg tagtagggaa cgatcgacat gcggccgagg
     3301 aaggccggct tgaagacctt gtcgagcccc ggcttgatcg tctcgacgat cttctccggc
     3361 gtcggcgccg tgtcggggtc ggcgcacatc ttcatcatcg cgtccgactc ggcgttcgtc
     3421 gtcaggagga tgacgcagtt cttgaagtcg atctcgcgtc cctccccgtc ctccatctgg
     3481 cccttgtcga agacctggta gaagagctcg aggacgtcgg ggtgcgcctt ctcgacctcg
     3541 tcgaggagga cgaccgagta gggcttgcgc ctcacggcct cggtcaggac gcctccctcg
     3601 ccgtagccga cgtagcccgg aggggagccc ttcagcgacg agacggtgtg cgcttcctgg
     3661 aactccgaca tgttgatcgt caccatgttc cgctcgccgc cgtagagcgt ctcggccagc
     3721 gcgatcgcgg tctcggtctt cccgacgccc gacgtgccga cgaacatgaa gacggcgatc
     3781 ggcttgcgcg ggtcggtgag ccccgcgcgg gaggtctgga tcttctgggc gatcgccgcg
     3841 agggcgtggt cctggccgac gacccgctcg ccgaggagct tcgcgaggtt gcggacggtc
     3901 tcgatctcgt cggcctgcat cttcccgacg gggatgccgg tccagcccgc gatgacctcc
     3961 gcgacggcct gggcgttcac gtcggcctgc atcatcggcg tgtcgccctg gacggtcttc
     4021 agctccgcct tcagacggtc gagctcgccc cggagcccct cgagccccgc gacgtccgcc
     4081 ccgccgacgg cggcttccag cttcccgcgg gtctccttga tctggttgac gagctcgatc
     4141 tccttcttgc actgctcgtc gaggacggcg aggcgggcct cggtctcggc cttcgcggcc
     4201 ttcgccttct cgatccgctc gccgtgcgcc gccccggccg ccgcctcgcg ctcgagggcg
     4261 tcgatctcgg cggtgagctg gtcgatccgg cgccggcagt cctcgatcgg ggccggggtc
     4321 gccgcctggc tgatgttcac cttcgcgcag gccgtgtcga gaacggagac gcacttgtcg
     4381 gggagctggc gccccgagat gtagcggtgc gagagcttga cggacgactc gaccgcctcg
     4441 tcgaggatcc ggagcccccc gtggtgcttc tcgaggttct tcgcgagggc ccgcatcatg
     4501 acgatcgcga tctcctcgct cggctcctcg accttgacga cctggaagcg gcgggcgagg
     4561 gccgcgtcct tctcgaagta cttcttgtac tcggcccagg tcgtcgcggc gatcgtccgg
     4621 agctcgccgc gcgcgagcgc cggcttcagg aggttcgcgg cgtcgttctg tccggccgtg
     4681 ccgcccgcgc cgatcatcgt gtgggcctcg tcgatgaaga ggatgatcgg cgtcggcgag
     4741 gccttgacct cgtcgatgac ctgcttcagc cggttctcga actcgccctt gatcccggct
     4801 ccggcctgga ggaggccgag gtcgagcgtc ctcacggcga cgttcttcag cggcccgggg
     4861 acgtcgccct gggcgatccg ctgcgcgaag ccctccacga ccgcggtctt cccgacgccc
     4921 gcctcgcccg tcaggatcgg gttgttctgg cgccggcgcg tgaggatgtc gatgacctga
     4981 cggatctcga agtcgcgtcc gaggatcggg tcgatcttcc cggcgcgcgc cttcgcggtg
     5041 aggtcttccg tgtactggtc gagggacttc gtctttcccg ggacgcccat cgcgacgtcc
     5101 ggtccgcccg ccgcgccggg cgcctccgag agggcctgcg cctcgcggtc ctccgcggag
     5161 cccttcacga cgtcggggaa cgtcttcgtc agctgctcga cggagatccc ggcgaggtcc
     5221 ttcgagatct cccgcgcgag gcgcgagagg tcgtcgtcgg tgaggaggac gtggacgagg
     5281 tggcccgacc tcacgcgcga ggcgccgtac tcgatcgagg cgacgaccca cgccttctcg
     5341 atgaggcggg gaatccgcgg ggagagggcg ggcgtccggg cattccccgt cttgaggcgg
     5401 tcgagggcgc gaacgacgtc cttcgcgaag cgcgacgcgt cgatctcgaa gcgccggagg
     5461 accttcgcga ggtccgtgtc gggcgcctcg agcagcttca ggaggaggtg ctcgacctcc
     5521 acgtcgtagt tcgtccggga gaggcagagg cccgccgccc cctcgagcgc ctgccggcag
     5581 gtgtcgttca gccggccgat cagggatttc aggttcacgc tcatctcgtc gtcctcccgc
     5641 gcaggggttt cgtcagttgt cgaccggaag gacgacgtct cccgcgtcct cccggggcgt
     5701 gtcggtggtg atccacgtcg tccacccgag catcgggggg agcgcggcgt tcgagtcgag
     5761 ggagcaggga gggacctcct ccttccgaag gacgagctgg acgtcgtagt cgaactcgag
     5821 gccggcgagg aaccggacga gctccgtgag ggggcggaac gccgggccga ccggcaggaa
     5881 cgagacgagc gcggagagcg gcagcggccc gagccggacg cggaacttcg actgcgagac
     5941 gaagaccgag gagccggcga ccagcgtccg gccgagctcg gagttcgccg cgccgagccg
     6001 cgtgacgttc tcctcttcca tcgggaacca ctgcccctgg aactggacga tccctgccgg
     6061 gacgcggaag tagtccctca ggaccgacgc gatcgcctcg cccgagtgcg gcttctgggc
     6121 gatgaggccg gcgtagagca ggagcgcctg gtcccgcacc gtcatccggc cgcgcaggcc
     6181 cggggtcccc atcccgatca ggtcgaagag gtactcggtg aaggagtcct cgcccgaccg
     6241 ctcgaaggcg atcgggaacc ggtacttctc ccacgcgcgg tagaagagcg acgtgaaccg
     6301 gtggtggaag aggtcgagga aggaccagag cgccgtgtcc ttgtaggaga cgcgctcgcg
     6361 cacgagctcg gtgtaaggat gcgggaggac gcccagcggc cccgtgaggc cgatgaagtt
     6421 gaccgtcatc tcgggcggcc ggtcctcctg gccgggaggc ggggccttca ggtcgacgat
     6481 ctcgctggga gggaacgcga gcgaagggtg cgttcggaac cggacgatct cgttcccggg
     6541 cctcacggcc cggccgacgc cctccttctc cggcgaggtc cgctcgagca ggcgaacggc
     6601 ctggaagaag gagaagcggt acggctcgga gaagaggagg tccttcatcc cgccttcaga
     6661 ggacgatctg ctcgccggcc cgcggctccc agcggcggac gacgccttcg cgctggcggc
     6721 tcgtcaggac gagctggctg aaggagttca cggaggagta gaggccgagg aagcgctcga
     6781 gcacctgggc gaagaggaag atgccggtgc cgacgaactg gttctcgtcg agctcgagcg
     6841 tgacctcgac gccgcggacg aagctcggct gcaggactcc gatcgagcgg aggacgcggc
     6901 gggcgccgac cttcgtgatc ccggcgatct gctgccgcgt gacggaggag tccgcgaaat
     6961 cgtagaggcg gaggatctcc tgcagcgcct cgggcccctg gccgtccgcc cgctcgacga
     7021 gggagaggag gttcagggag aggtgcgaga ggagcctcca ttgcaggcct cggcggagcg
     7081 gcggccgcac ggtcggcgtc ggccggcgca ggcaccggat gcccgagaag accccggccc
     7141 cttccagctg gaagtcggcc tccccgccct cgaacgggag ccgcgaggga aggtcccggt
     7201 tcgtgcagag cgtctcgacg gtcagcgtat cggcgtcgac ctccgccggg tcgaagccga
     7261 ggtcgacgag cgagaggtag gcgtcggtcc cgtcgtcgtc cttgcgggcc gacggacggc
     7321 ggctcatgta ccagaacgcg ggagcctggc ccttctcgaa cccgtgccgg tacgagtaga
     7381 acggctcgta gctcgtgacc ttgtcgcccc ccttcgcgac gctcgtgacg gcctcgacgg
     7441 agtagacctc catgacgtcc tgccgccgca cgtcggggat cacgcggtac tcgggctgca
     7501 ggtgcgtcag ccggatcggc tcggcgacct gccggaagag gttcgcgacg ggggtgcagt
     7561 tgaggcggaa ggtctgggcg gtgacgttgc gctcgagcgg gaagtcgcgg tcgaagtaga
     7621 ggaggatctc gaggacgtcg tcgaagtcgt cccgggcggc gcccgcgagg ccgttcacgt
     7681 cgaagaagaa gaacttctcc gggaacgcga agtactccgc gagaaggcga taccccagga
     7741 aggagcggtc cgtgtagggg aggagcccct cgtcgcggcc gaatccgacg ccggcgacgg
     7801 aggtcttcgg gaggcggacg ggcgacggca cgtcgcgcgg tccgcccggc cggagctcga
     7861 cggcgacgag gtggttgtgg atcagctcgt ggagcgcgta gacgagcttt ccttctccct
     7921 ggaggaagaa gcggagagag tcgagaggct tctcctccga ctcggagacc ttctccttca
     7981 gctcgcgcag gggcgcgccg ccgaacgtct tcagctcgag gcggaggacc gtccggaccc
     8041 cctcttccgg cccgacgccc gggatcggca gctcgaagcg cgcctggcgc atctcgaccg
     8101 gccagatgcg gaccgggtag gccgtccgga accggcacga cgtccccgcg acggaacggg
     8161 agtagagcat cgagccgcgc gggatcttct gtcccgactg gaggttgacc tgtgacgggt
     8221 cgaggacgaa ctggacgatc gacagggacg ggatcggcgc gaggaggtgg ggatagagga
     8281 cgttgaggag cgactcggtg atctcgggga actcgtcgtc gaccttcagg tggacgcgcg
     8341 aggcgaggaa cgcgaacgcc tcgatgagcc gctcgacgtg cgggtcctcg caggtgtcgg
     8401 gctccagctg gagacggccc gcgatcttcg ggtacttctc cgcgaactcg gcccccatct
     8461 ggcggaggaa ggagagctcg cgctcgtagt acccgaggag ctcgtcgcgc atcactcccc
     8521 cttcacgcgg aactccccgc tgccgatctg gagcagggtg tcgaacgaga ccggctcggg
     8581 agccgggtcg accttgagcc ggccgtcgat gcggaacttc agcgagaggc ccggtccggg
     8641 ctcggggtcg acggtcacga cgacgccctc gaggcgcggc tcgaacgtct cgaggaccct
     8701 ctcgaccgcg cgccggagct tcgccttgtc ggccgggctc cgccccgaca gggccgtgat
     8761 gtcgggcagc ccgtagacca cgatggagga ccagacctcc ttcagctcgg gcggcacgac
     8821 cgccgtcgcc cgcgtgtt
//
LOCUS       DAOONL010000132         8833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9279_length_8833_cov_17.922420, whole genome shotgun sequence.
ACCESSION   DAOONL010000132 DAOONL010000000
VERSION     DAOONL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_8833_cov_17.922420"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLP50_14415"
     CDS             <1..668
                     /locus_tag="PLP50_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA52786.1"
                     /translation="AVVEDDEDLAFTLVYSLRKDGRFEPVRFAGGLVALDELTERPPD
                     LVLLDLNLPDVDGLTICRELRKHDATRRVPILMLTARAEERDKLLGFDLGADDYVTKP
                     FSMKELLARIAARLRRGDGGASAPDEVFDDGKIRIDRGRHLVERGGKSVHLTKKEFDL
                     LWLLIRSGGRVVSRETILSRLWDYAADVETRTVDVHVRSIRKKLGDDVVETVVGVGYR
                     YPG"
     gene            665..2044
                     /locus_tag="PLP50_14420"
     CDS             665..2044
                     /locus_tag="PLP50_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52787.1"
                     /translation="MKPSVPLRLRPAAEALALGAITVAVVGLEPRALLAGALVAAAFF
                     AIRLRRMRELDRVREGVAVAATGTRPEEWRGLSPETSEVLEALHRFADLVAREREGSE
                     RARVLARTVFEEVPVGLVVVDRGLAVLDVNAAARVLFGAPPSAPCAALHDLVRAPEVH
                     ALFRSLEGNGGETPAPIVVRIEGREGGERFLEVAARAVPGRPGAGEPAAIGVARDVTE
                     RERTERMRRQFVADVSHELRTPLATVRAAAEALSGEEGLGGDGARLVGIVERQASQMQ
                     DLVSDLTDLSQIEAGSVTIQLEVVPLAGLLASVAKDLEAAARAREVEVAIEAPEGLAV
                     HGDRRRLAQVFRNLLDNALKFSPAGSRVDLRAEGGTEAGAQVARVHVVDRGIGIPASE
                     QVKIFQRFYRIDPSRAKSTPGTGLGLSIVKHLLILHGGTVRVESEPLCGSRFTVTLPA
                     AAAALKEAS"
     gene            2041..2721
                     /gene="phoU"
                     /locus_tag="PLP50_14425"
     CDS             2041..2721
                     /gene="phoU"
                     /locus_tag="PLP50_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPA52788.1"
                     /translation="MKEHFSELLERLRRQLIGMGAEVESQIRVAVEALVEGDAAKARS
                     VCERDPVVNDLEVRNEEAVIQLLATQQPVASDLRLLVAAVRINADLERIGDHAVNIAQ
                     AAERLAGVRPFKPWIDIPHMAEVVRGMLRDALDAFVRRDTALAKKVLERDDVVDAKNL
                     SLIRELLTYMAENPALISRSIEIMLISRNLERVADLATNIAEETIFIAEGLVIKHQGT
                     RPEEPDAR"
     gene            2732..4204
                     /locus_tag="PLP50_14430"
     CDS             2732..4204
                     /locus_tag="PLP50_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52789.1"
                     /translation="MTEAARLRAAALLRRAFVVVLVWAAFATGFLGAERTFRAGTPVS
                     LAAGAAVAAAFLALVLGGGDHLAAIVSILAERAERVPAGRWLAGCVAAAAAVRLAWGA
                     LLTPRQVSDMELYYAFARSLAERGVFEHGGDRVFGPPGVSLLLAPLVKLFGAGTYLPL
                     AVNVALAAGTVLVTGALALRVADGRTMRVAALLVALWPNAVLLSGLVSKELLVLFLLP
                     LGALLYLRATEGTEADRRGLLLGAGLALGAATLAQPNTLVLLPAFAAVELLRAVPFRV
                     ALLRLGLLGAGTALAVSPWTIRNALLFGRFVPVSVNFGHAMLVGNHDGADGGFRPVRT
                     PYLGMPEIEYDRLALRKALDWIRENPGRFLALVPRKQMVFLGDDGDGAYHALKWAYGI
                     GDARYALAKAASNGGWVGVLALAAVALSGRRGREADRDPRLALGILFFFCLLALLSVT
                     ESGSRHHLALSPFLALLAGLLARPAAGAASHGGRERATEG"
     gene            complement(4216..7515)
                     /locus_tag="PLP50_14435"
     CDS             complement(4216..7515)
                     /locus_tag="PLP50_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPA52790.1"
                     /translation="MTPLVDGAARAAAVTELERSLLVEASAGTGKTRTLIERIVRLVV
                     EAERPLSSIAAMTFTEKAAGEMKERLRLRLDEVSRSAEDAEQRARAAVARRDLDSAEV
                     STIHSFCARLLRERPVEAHVDPEFVTPEEGVALELFEEVFAAWVDEAARAEGPVADAF
                     RRGLSPDALRALAGTIHAERSLVRAGTVPDDPLPFVRAVLLTLVAEGERIVDDVPPDL
                     RKDPKVLLVRSAIDEVRDLLPLGIDELVAFRPVTDISFRPGKRNLFEGDAKKRAEEFR
                     KAFRELPGQLASLPDERLLARLVEEVRDRFLQRLEDEKRRRGLLDFDDLLLAARDLLR
                     SSRAAREHFQARFPHLVVDEFQDTDPVQAEIVLRLAAPEGEQDGAPWEGLSPAGGALF
                     LVGDPKQSIYRFRRADVETYRRVAGSLGENARLSLVTNFRSAPRILSFVNALFSRAFD
                     GSPDEPWEVENAPLVPRPEAAADPDAGEVVYLAAPPVPAPRDDEEEGPEDGGDGGDEA
                     ADAHATVQEARAVANLLRSRFASGGYRGVAVLLGSNDAIDTFQDVFREAGIPAVLDGG
                     VSFYRREETAAVVAALRAVDDPSDGISTVAALKSFLFGLTDVELLDAAETGTRFDDPA
                     TAPALGPVAECLAFLSGLRSRRLERPLAETLLELLSTRRAFAAVESGAVVNPLQASAN
                     LERLLVLARSLDAEGLPFREAVARLSLRLEERTAEPRAFEEDEDAVRLLTLHKSKGLE
                     FETVVVAGLGFRDRERAASGRSLLYDHAGGLLAFRVPLGGRSLGTPGLRIVEGRDDLR
                     RRAEEKRLLYVALTRPRKTLVLSWFRRRRRLKSGEVSDAIGKSLLAPIAFAEGPPPEL
                     APLVEVVAPDVSVPPPPKRPAAPDDSVDLVAEMAAAEARLHRARAAASRPLRRSGEKE
                     TGPAPLPAAGPERESPEDLPALERHEEIHEAAVRLGTAVHEAMEALLDPARTPPSSAA
                     EAVATVTVPLGGPARAEAVRLVERLLAHPVAARARAARRRFVELPILFRDDALEGAPL
                     VEGKIDLLFEEEDGWVVVDWKTDRLPTPAVRAEREALYAPQLASYARALVALLGPGTR
                     VKETLLAFARG"
     gene            complement(7512..>8833)
                     /locus_tag="PLP50_14440"
     CDS             complement(7512..>8833)
                     /locus_tag="PLP50_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPA52791.1"
                     /translation="VLLAARREAATDRPRLLSPFLLDLLERRAGRPLSEELLSDPTTA
                     SRLGVRWIRLGRASAAEPPVDADEALRRALGRAPKLAAALPPGLEPLDRALRRGRARA
                     EPRYTEFEGRLGRAPRALRFEGRAVTPSRLERLASCAYKAFLVEALGLAAPEDAPGAL
                     VLDRRTAGELAHAALDAVAKLALRSGRALSETLRARGPAEAERQLRRFLVDWQIDLPP
                     VLAEAASSRLASLLRAVEELEAARPDVLPVAGSEVGFGPASGLAAVAGEGVARLPLAG
                     RIDRLDRAGEAARVVDYKFANPKPFGKTNRKGWRIVGGEKVQLAAYALAARALGATEV
                     SSEYLFVADGKKGGAPEATSVAFGAAETGEAFASFHRAVALLDAAVLSGDLLPRTSSL
                     SAGDSLCSFCEVAAVCGPGHRRVYDAKREAERAGRPGQPLFVLEEVP"
BASE COUNT         1122 a         3123 c         3473 g         1115 t
ORIGIN      
        1 tcgccgtcgt cgaggacgac gaggacctcg ccttcacgct cgtctacagc ctccggaagg
       61 acgggcgctt cgagccggtt cgcttcgcgg ggggactcgt cgcgctcgac gagctgaccg
      121 agcgcccgcc ggacctcgtc ctcctcgacc tgaacctccc cgacgtcgac ggcctgacga
      181 tctgccggga gctccggaag cacgacgcga cgcgacgcgt gccgatcctc atgctgacgg
      241 cccgcgccga ggagcgcgac aagctcctcg gcttcgacct cggggcggac gactacgtca
      301 cgaagccgtt ctcgatgaag gagctcctcg cccggatcgc ggcgcggctc cggcgcggcg
      361 acggcggggc ctcggcgccg gacgaagtct tcgacgacgg gaagatccgg atcgaccggg
      421 ggcgtcacct cgtcgagcgc ggcgggaaat ccgtccacct gacgaagaag gagttcgacc
      481 tcctctggct cctgatccgg agcggggggc gggtcgtcag ccgggagacg atcctctcgc
      541 gcctctggga ctacgccgcc gacgtcgaga cgcggacggt cgacgtccac gtccgctcga
      601 tccggaagaa gctcggggac gacgtcgtgg agacggtcgt cggcgtcggc tacaggtacc
      661 cgggatgaag ccttccgtcc cgctccggct ccggccggcc gccgaggccc tcgcgctcgg
      721 ggcgatcacc gtggcggtgg tcgggctcga gccgcgcgcc ctcctcgcgg gcgctctcgt
      781 cgcggcggcg ttcttcgcga tccggctccg ccggatgcgg gagctcgacc gggtccgcga
      841 gggggtcgcc gtggcggcca cggggacgcg cccggaggag tggcggggcc tctcgccgga
      901 gacgtcggag gtcctcgagg cgctccatcg cttcgccgac ctcgtcgcgc gcgagaggga
      961 ggggagcgag cgggcgcgcg tcctcgcccg gacggtcttc gaggaggtcc ccgtcggcct
     1021 cgtcgtcgtc gaccgcggcc tcgccgtcct cgacgtgaac gcggcggccc gcgtgctttt
     1081 cggagcgccc ccgtcggcgc cctgcgccgc cctccacgac ctcgtgcgcg ccccggaggt
     1141 gcatgccctc ttccgctcgc tcgaggggaa cggaggcgag acacccgcgc ccatcgtcgt
     1201 ccggatcgag gggcgggaag ggggcgagcg cttcctcgag gtggccgcgc gggcggtgcc
     1261 ggggcggccg ggcgccgggg agccggccgc gatcggcgtg gcccgcgacg tgacggagcg
     1321 ggagcggacg gagcggatgc ggcggcagtt cgtcgcggac gtctcgcacg agctccggac
     1381 gcccctcgcg acggtccgcg cggcggccga ggcgctctcc ggcgaggagg ggctcggagg
     1441 ggacggcgcg cgcctcgtcg gcatcgtgga gcggcaggcg tcccagatgc aggacctcgt
     1501 ctccgacctg accgacctct cccagatcga agcgggctcc gtgacgatcc agctcgaggt
     1561 cgtcccactc gcgggcctcc tcgcgagcgt ggcgaaggac ctcgaggcgg ccgcccgggc
     1621 gcgagaggtc gaggtggcga tcgaggcgcc ggaggggctc gcggtccacg gcgaccggcg
     1681 gcgactggcg caggtcttcc ggaacctcct cgacaacgcc ctcaagttct cgccggccgg
     1741 gtcgagggtc gatctgcggg ccgagggcgg gaccgaagcc ggtgcgcagg tggcccgggt
     1801 ccacgtcgtc gaccgcggga tcgggatccc cgcgagcgag caggtgaaga tcttccagcg
     1861 cttctaccgg atcgacccgt cgcgcgcgaa gtcgacgccc ggaacgggcc tcggcctctc
     1921 gatcgtcaag cacctcctca tcctccacgg cggtacggtc cgcgtggagt ccgagccgct
     1981 ctgcgggagc cgcttcaccg tcaccctccc ggccgccgcc gccgccctca aggaggcctc
     2041 atgaaggaac atttctcgga gcttctcgag cggctccgtc gccagctgat cgggatgggc
     2101 gcggaggtgg agagccagat ccgcgtcgcc gtggaggcgc tcgtcgaggg cgacgccgcc
     2161 aaggcgcgtt ccgtctgcga gcgcgacccg gtggtcaacg acctcgaggt gcggaacgag
     2221 gaggcggtca tccagctcct cgccacgcag cagccggtcg cgagcgacct gcggctcctg
     2281 gtggcggcgg tccggatcaa cgcggacctg gagcgaatcg gcgaccacgc cgtgaacatc
     2341 gcgcaggccg ccgagcggct cgccggagtc cggccgttca agccttggat cgacatcccg
     2401 cacatggcgg aggtcgtccg cgggatgctg cgcgatgccc tcgacgcgtt cgtgaggcgc
     2461 gacacggccc tcgccaagaa ggtcctcgaa cgcgacgacg tcgtcgacgc gaagaacctc
     2521 tcgctcatcc gcgagctgct gacctacatg gccgagaacc cggcgctcat cagccggtcg
     2581 atcgagatca tgctcatctc gcggaacctc gagcgggtcg ccgacctcgc gacgaacatc
     2641 gcggaggaga cgatcttcat cgccgagggg ctcgtcatca agcaccaggg gacgcgcccg
     2701 gaggagccgg acgcgcgctg acgggacgcc ggtgaccgaa gcggcccggc tccgggcggc
     2761 ggcccttctc cggcgagcat tcgtcgtcgt cctcgtctgg gcggcgttcg cgaccggctt
     2821 tctcggggcg gagcggacgt tccgggccgg gacgccggtc tccctcgccg cgggggcggc
     2881 agtcgccgcg gcgttcctgg ccctcgtcct cggcgggggc gaccacctcg cggcgatcgt
     2941 ctcgatcctg gcggaacggg cggagagggt ccccgcaggg cgctggctcg cgggatgcgt
     3001 cgcggccgcc gcggcagtcc gcctcgcctg gggcgccctc ctgacgccac ggcaggtctc
     3061 cgacatggag ctctattacg cgttcgcccg gagcctcgcc gagcgcggcg tcttcgagca
     3121 cggcggggac cgggtcttcg gcccgcccgg cgtctcgctc ctcctcgcgc cgctcgtgaa
     3181 gctcttcggc gcggggacgt acctccctct cgccgtgaac gtcgcgctgg cggcggggac
     3241 ggtcctcgtg acgggcgccc tggcgctccg cgtcgcggac gggcggacga tgcgggtcgc
     3301 ggcgctcctc gtcgcgctct ggccgaacgc cgtcctcctc tcggggctcg tcagcaagga
     3361 gctgctcgtc ctcttccttc tcccgctcgg cgcgctcctc tacctgcgcg cgacggaggg
     3421 aacggaggcg gaccgccggg ggctccttct cggcgcgggc ctcgccctcg gggcggcgac
     3481 gctcgcacag ccgaacacgc tcgtccttct ccccgcgttc gcggcggtcg agctcctgcg
     3541 tgcggtgccc ttccgcgtcg cgctcctccg gctcggcctc ctcggcgcgg ggacggcgct
     3601 cgcggtctcg ccctggacga tccggaacgc gctcctcttc ggtcggttcg tcccggtctc
     3661 cgtgaatttc gggcacgcga tgctcgtcgg gaaccacgac ggcgccgacg gcggcttccg
     3721 gccggtgagg acgccgtacc tcgggatgcc cgagatcgag tacgaccgac tggccctccg
     3781 gaaggcgctc gactggatcc gcgagaaccc gggccggttc ctcgcgctcg tcccgcgcaa
     3841 gcagatggtc ttcctcggcg acgacggcga cggcgcctac cacgccctga agtgggctta
     3901 cgggatcggc gacgcgcgct acgcgctcgc gaaggcggcc tcgaacggcg ggtgggtcgg
     3961 cgtcctcgcg ctcgcggccg tcgcgctgtc ggggcggcgg ggccgcgagg ccgaccggga
     4021 cccccggctc gcgctcggca tcctcttctt cttctgcctc ctcgcgctgc tgagcgtcac
     4081 cgagagcggc tcgaggcacc acctcgccct ctcgccgttc ctggccctcc tcgcgggtct
     4141 gctcgcccgg ccggcggcgg gcgcggcgtc gcacggcggg cgcgagcggg cgaccgaggg
     4201 gtaaagccgg gccggtcagc cgcgggcgaa tgcgaggagc gtctctttga cccgcgttcc
     4261 ggggccgagg agcgcgacga gggcgcgggc gtagctcgcg agctgcggcg cgtagagcgc
     4321 ctcgcgctcg gcgcgaacgg ccggcgtcgg gaggcggtcg gtcttccagt ccacgacgac
     4381 ccagccgtcc tcctcctcga acaggaggtc gatcttcccc tcgacgagcg gcgcgccctc
     4441 gagcgcgtcg tcgcggaaga ggatcggcag ctcgacgaag cggcggcgcg cggcacgagc
     4501 gcgggcggcg acggggtgcg cgaggaggcg ctcgacgagc cggacggctt cggcgcgcgc
     4561 ggggccgccg agagggaccg tgaccgtggc gacggcctcg gccgcggagg agggaggcgt
     4621 tcgcgcgggg tcgaggagcg cctccatcgc ctcgtggacg gcggtaccga ggcggacggc
     4681 ggcttcgtgg atctcctcgt gccgttcgag ggccgggagg tcctcgggcg attcgcgctc
     4741 cgggccggcg gccgggagcg gtgccgggcc ggtctccttc tcgcccgagc gacggagcgg
     4801 gcgtgaggcc gcagcccggg cccggtggag gcgagcctcg gcggccgcca tctcggcgac
     4861 gaggtccacg gaatcgtccg gggccgcggg cctcttcggc ggcgggggga cggagacgtc
     4921 gggcgcgacg acctcgacga ggggggcgag ctcgggcgga gggccctcgg cgaacgcgat
     4981 cggcgcgagg aggctcttgc cgatcgcgtc cgagacctcg ccggacttca ggcgccggcg
     5041 cctccggaac caggagagga cgagcgtctt ccgcggccgg gtcagggcga cgtagaggag
     5101 gcgcttctcc tcggcgcgac ggcggaggtc gtcgcgaccc tcgacgatgc ggaggccggg
     5161 cgtcccgagc gaccgtccgc cgagaggcac gcggaacgcc agcaggccgc ccgcgtggtc
     5221 gtacaggagc gacctccctg acgccgcccg ctcccggtct cggaagccga ggcccgcgac
     5281 gacgacggtt tcgaactcga gccccttcga cttgtggagc gtcaggaggc ggacggcgtc
     5341 ctcgtcctcc tcgaacgcgc gtggctcggc ggtccgctcc tcgaggcgga gggagaggcg
     5401 ggcgaccgcc tcccggaagg ggagcccctc cgcgtcgagg ctgcgggcga ggacgaggag
     5461 ccgctcgagg ttcgcggacg cctggagcgg gttgacgacg gcgcccgact cgacggccgc
     5521 gaaggcgcgg cgcgtggaga ggagctcgag gagcgtctcg gcaagaggcc gctcgaggcg
     5581 gcgcgagcgc aggcccgaga ggaacgcgag gcactcggcg acgggtccca gggcgggggc
     5641 cgtggcgggg tcgtcgaatc gcgtgccggt ctcggcggcg tcgaggagct cgacgtcggt
     5701 gaggccgaag aggaacgact tgagggcggc gacggtcgag atgccgtcgg aggggtcgtc
     5761 gacggcgcgg agggcggcga cgaccgccgc cgtctcttcg cgccggtaaa agctgacccc
     5821 gccgtcgagg acggccggga tccccgcctc gcggaagacg tcctggaacg tgtcgatggc
     5881 gtcgttcgag ccgaggagga cggcgacgcc gcgatagccg ccggaggcga agcgcgatcg
     5941 gaggaggttc gcgacggcgc gggcttcctg gacggtcgcg tgcgcgtcgg cggcctcgtc
     6001 ccccccgtcc cccccgtcct ccgggccctc ctcctcgtcg tcgcggggcg ccggcacggg
     6061 cggcgcggcg aggtagacca cctctcccgc gtccgggtcg gccgcggcct cggggcgcgg
     6121 gacgaggggg gcgttctcca cctcccaggg ctcgtcggga gagccgtcga acgcgcgcga
     6181 gaagagggcg tttacgaacg agaggatccg gggagccgag cggaagttcg tgacgagcga
     6241 gagccgggcg ttctcgccga ggctcccggc gacgcgccgg taggtctcga cgtccgcccg
     6301 gcggaagcgg tagatcgact gcttcgggtc gccgacgagg aagagcgccc cgccggccgg
     6361 ggagagcccc tcccacggcg cgccgtcctg ctctccctcg ggcgcggcga gccgcaggac
     6421 gatctcggcc tgcacggggt cggtgtcctg gaactcgtcg acgacgaggt gggggaagcg
     6481 cgcctggaag tgctcgcgcg cggcgcggga gctgcggagg aggtcgcgtg ccgcgaggag
     6541 caggtcgtcg aagtcgagga ggccgcggcg cctcttctcg tcctcgagcc gctggaggaa
     6601 ccggtcgcgg acctcttcga cgaggcgggc gaggagtctc tcgtcgggga gggacgcgag
     6661 ctggccgggg agctcccgga acgccttccg gaactcctcc gcccgcttct tcgcgtcgcc
     6721 ctcgaagagg ttccgcttcc cggggcggaa gctgatgtcg gtaacgggcc ggaacgcgac
     6781 gagctcgtcg atcccgagcg ggaggaggtc gcggacctcg tcgatcgccg agcggacgag
     6841 gaggaccttc gggtccttcc ggagatcggg cgggacgtcg tcgacgatcc gctcgccctc
     6901 ggcgacgagc gtcaggagga ccgcgcggac gaacgggagc gggtcgtcgg ggacggtgcc
     6961 cgcccgaacg agcgaacgct cggcgtggat cgtccccgcg agcgcgcgga gggcgtcggg
     7021 cgagaggccg cgccggaacg cgtcggcgac ggggccttcc gcccgggcgg cctcgtcgac
     7081 ccacgcggcg aagacctcct cgaagagctc gagggcgacc ccctcctcgg gcgtgacgaa
     7141 ctcgggatcg acgtgcgcct cgacgggccg ctcgcgcagc agccgtgcgc agaaggagtg
     7201 gatcgtcgag acctcggccg agtcgaggtc ccggcgggcg acggcggcgc gtgcccgctg
     7261 ctcggcgtcc tccgcggagc gggagacctc gtcgagcctc aggcggaggc gttccttcat
     7321 ctcgcccgcc gccttctcgg tgaaggtcat cgccgcgatc gaggagagcg ggcgctcggc
     7381 ctcgacgacg agccggacga tcctctcgat gagcgtccgc gtcttgccgg tcccggccga
     7441 ggcctcgacg aggagcgacc gctcgagctc ggtgacggcc gccgcgcggg cggccccgtc
     7501 gacgaggggc gtcacgggac ctcctccaga acgaagagcg gctgccctgg ccggcccgcc
     7561 cgctccgcct cgcgcttggc gtcgtagacg cgccggtggc cggggccgca gacggctgcg
     7621 acctcgcaga aggagcagag gctgtcgccg gcggagagcg aagacgtcct cggcaggagg
     7681 tcgccggaga ggaccgcggc gtcgaggagg gcgacggcgc gatggaacga ggcgaaggcc
     7741 tcgcccgtct cggcggcgcc gaaggcaacg gacgtcgcct cgggtgcgcc ccccttcttc
     7801 ccgtcggcga cgaagaggta ctccgaggag acctcggtcg cgccgagcgc gcgggccgcg
     7861 agggcgtagg ccgcgagctg gaccttctcg ccgccgacga tccgccaccc cttcctgttc
     7921 gtcttcccga acggcttcgg gttcgcgaac ttgtagtcga cgacgcgggc ggcctcgccg
     7981 gcgcggtcga ggcggtcgat ccggcccgcg agcggaagcc gcgcgacgcc ctcgcccgcg
     8041 acggccgcga ggcccgaagc cgggccgaag ccgacctccg aaccggcgac ggggagaacg
     8101 tccggacggg cggcttcgag ctcctcgacc gcgcgaagaa gagaggcgag acgcgaggag
     8161 gcggcctcgg cgaggacggg cggcaggtcg atctgccagt cgacgaggaa ccggcgcagc
     8221 tgccgctccg cctcggccgg gccgcgcgcg cggagggtct cggagagggc ccgtccgctt
     8281 cggagagcca gcttcgcgac ggcgtcgaga gcggcgtgcg cgagctcgcc ggcagtccgc
     8341 ctgtcgagga cgagcgcgcc gggggcgtcc tccggtgcgg cgaggccgag cgcctccacg
     8401 aggaacgcct tgtaggcgca ggaggcgagc cgctcgaggc ggctcggcgt gacggcgcgg
     8461 ccctcgaagc ggagcgcgcg gggagcgcgc ccgaggcgcc cctcgaactc ggtgtagcgc
     8521 ggctcggcgc gggcgcggcc gcggcggagg gcgcggtcga gcggctcgag gccgggtggg
     8581 agggcggccg cgagcttcgg cgcccggccg agggcgcggc ggagcgcctc gtcggcgtcg
     8641 acgggcggct cggccgccga ggcccggccg aggcgaatcc accgaacccc gagccgggac
     8701 gccgtcgtgg ggtcggagag gagctcctcg gagagcggcc ggcccgcgcg ccgctcgagg
     8761 aggtcgagga ggaaggggga gaggaggcgg ggccggtccg tcgcggcctc gcggcgcgcc
     8821 gcgaggagga cga
//
LOCUS       DAOONL010000133         8524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9726_length_8524_cov_13.321171, whole genome shotgun sequence.
ACCESSION   DAOONL010000133 DAOONL010000000
VERSION     DAOONL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_8524_cov_13.321171"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLP50_14445"
     CDS             complement(<1..1478)
                     /locus_tag="PLP50_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HPA52792.1"
                     /translation="MASPRLLALAVPLFPLAARLRAEPALRDEALAVTEGNGSAARVV
                     AATRSARAAGVKPGMTLPQARALVPRLLARGRDPHAESAAQEALLDVAGRFSPRVEDA
                     GDGLAFLDLAGLAPRATPDPLAFERDLALDLLRAADKDGLPARAGVASSKRAARIASG
                     LPRTPVVVPPGEEARFLAPLPVERLAPELSLSVALSRWGVRTAGELARLPAAEVGTRL
                     GEPGRRLHEAARGLDPEPFVPRLPALAFTEGASLDWPVVALEPFLAFALAALERLVRR
                     LAAEALGCARLELSLHLEPEGCDVRAIDLPGPTRDAKTLLSLLRQSLDARPPGAPVTG
                     FALAAHPDRPRRGQLTLFGPPEVSPDELAGTLARLLVLLGDGRAGSPRPDPGHVPGRF
                     ALVPYAPPPAPLLTPPLRPARGLLAIRALRPAVELEVLTHADTPVSLSALPPSPTPVH
                     GRVRVAAGPWTLEDGWWSDTPAARDYWDVELDGGGLYRLYRDR"
     gene            complement(1497..2459)
                     /locus_tag="PLP50_14450"
     CDS             complement(1497..2459)
                     /locus_tag="PLP50_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52793.1"
                     /translation="MSSLPVPLPADVASRLFRAADLPRRGAALRAGDRLATGVAPLDA
                     LLDGGLPRGALVELTGRSGGRFALVLATLAAATSRGETAALLDLGDHLDPSSAEEAGC
                     DLARLLWVRPQRFRDALLAAETVAAAGFRLVVLDAGLALPSSRQRDAAPWLRLARAAA
                     PRRVAFLLSSPVPLAGLAAEALVGVSARGLFRSAGSVRFLAGAAARVSLLKVRGGRPA
                     EAPPLALSPLAAPLAERLLPEIASPPAPARAVAGTPRTAPTSSPSASPAGAPSLLSPP
                     SLHLARTAAAPSVPAASSLAVPSPFAGPSPSPATPSQRHLAPCA"
     gene            2684..3472
                     /locus_tag="PLP50_14455"
     CDS             2684..3472
                     /locus_tag="PLP50_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPA52794.1"
                     /translation="MNHVVCIAYVPDTETKIKIGADGKSIDEADVKWIVSPYDEYALE
                     GAIKAKEEKGGTVTVVTYGPARADVGLRECLARGADEAVRVASDGLGEADSLGVAMAL
                     AGAIKALPCDVLWLGNKGVGTDAQTMVPMLAELLDLPHANLVTKLEWKDGGVVAYREI
                     EGAREVVEMPLPCVIGAQKGLNEPRYASLKGIMAAKKKTIAVKQPAEVGVDPAATTGE
                     KAAVRWTKLELPPARQAVKLIPADDPAKAADELVRLLRDEAKVL"
     gene            3501..4475
                     /locus_tag="PLP50_14460"
     CDS             3501..4475
                     /locus_tag="PLP50_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPA52795.1"
                     /translation="MSNVLVFIEQRDGKIRKASFEALTLGREVAAKTGGALGAVVAGS
                     GIGGLAPELAAYGAAKVFVADKPELALYSCEGYTAALDAAVAAFAPAVVLVPATSMGK
                     DVAPRLAARRKVGLLSDVMSLEVEGGRLVATRSVYSGKARATVAADPGAALQIATPRP
                     NVFPASKGAAGAGEVVALDFSVTIRAKVVKLEMPEAGELDVAEADKIVSGGRGIKGPE
                     GWPVLRALCHELGAALGASRAAVDAGWIGHEHQVGQTGKVVSPTLYVACGISGAIQHM
                     AGMGSSKVIVAINKDADAPIFKAATYGIVGDLFQIVPALTEAVRKAKA"
     gene            complement(4584..6404)
                     /locus_tag="PLP50_14465"
     CDS             complement(4584..6404)
                     /locus_tag="PLP50_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52796.1"
                     /translation="MRKTSSFLVLTVLLGLAAATPAVALAALAAPQGTDLWGGPGAKG
                     TGNQGARFDTVVYVTGPSPAVGAVEFLAGGKLLATVPFTFAANGVALVTAPEAVAEKG
                     AFLYRVRSDVPVSAWSETYNDASAGRYGVSLGAAAPSELLGPGDEATGGGAEASSESG
                     AGHARTNAGVVCVDTASQPCRLEVALFSSSALVGSGTIEAAPGSAVQASLATLIPAAA
                     GKAGLALRLRLLSGQGYPYAIRNDNQTSDGTAIPLAVKRNAFSTAPTIASFTASPATG
                     CAPLETTFSWETSGAANVSISGVGNDLPATGSVKTTVHATGDFILTATSSTGQSTIQP
                     LRITITPSTPAPTPAPASATLPTSGVVTGILPVGTGPVTVEFVSRESTGSTFTVQGTS
                     FIYTAGTTPGTDVVKITATGSCGGASAEFTAHVVLPGAPRILSFEAIPERGCAPSTNF
                     LLTWRTENAIGVYISGLDENFVANGGVEVTLTATASFVLTAYSLDGAETTATVTVPID
                     PQLYVPTITPGAVAVAGGTQTIIDVNPASVPLLDDVRFAVVQSQSRGMFSRVPNVPGR
                     FIYRAGPYTGIDIIRILWVNGCGIGYTDFTANVTGETPEP"
     gene            6537..>8524
                     /locus_tag="PLP50_14470"
     CDS             6537..>8524
                     /locus_tag="PLP50_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPA52797.1"
                     /translation="MMPTEASWLGVPGTLVFALVLIGAIAAFAYTASRRWQLLTIGGP
                     EDVRWDRPVERLKGLFELGIFQKKMWWDGYAGLYHMLIFSGFVVLSVRTLSLVFEGLF
                     PKAGMPFLPAGAWQAYLLLKDVVLVTTLVGVVLALGRRYVFRKERLDPSFDAGLILVL
                     IGFLMATDLLAGAAKFALVPEHASAWEPFTAGLSGLLAGAAPATLQAVFSWCWWLHLV
                     AILVFLNYLPFAKHFHVITALPNIFLRKLDAPGRLPTVDIEKAAEAEKFGVAKVEDLT
                     WKQRLDLFTCTECGRCREVCPTHLTGKPLTPKGFLKDVRSALYTVAGDLVDTAEGRAD
                     VAAQARLEERKPLIGGWVDEETIWACTTCRYCESACPVTITYTDKIVEMRRHLVLEKS
                     EFPKEAQTAFNGMERQGNPWNLPSADRDAWTGELDFEVPVLGAMDESERAQVEVLFWV
                     GCAGSYEDRGKKVSRALARLLHEGGVKFAILGAEETCNGDSARRLGNEYLFQTLAQQN
                     VETMNGYGVKRIVTNCPHCFNTLKNEYPDFGGTFEVIHGTELVALLLAEGRLQLTESV
                     AKSISFHDACYLGRHNDVYDAPREVLAAIPGLTLTELPRTGKNGLCCGAGGGRMWLDE
                     KIGTRINQLRYEEIESAGTDLVGVSCPFCMVMLGNAQT"
BASE COUNT         1236 a         2717 c         3537 g         1034 t
ORIGIN      
        1 cggtcgcggt agaggcggta gaggccgccg ccgtcgagct cgacgtccca gtagtcgcgg
       61 gcggcggggg tgtcggacca ccagccgtcc tcgagggtcc aggggccggc ggccacgcgg
      121 actcggccgt ggacgggcgt cggagacgga gggagggcgc tgagggagac gggggtgtcg
      181 gcatgggtga ggacctccag ctcgacggcg gggcggagcg cgcggatcgc gaggaggccg
      241 cgcgcggggc ggaggggcgg ggtgaggagc ggcgccgggg gcggggcgta ggggacgagg
      301 gcgaagcggc ccgggacgtg gcccgggtcg ggtcggggcg agcccgcgcg cccgtcgccg
      361 aggaggacga ggaggcgggc gagggtgccg gcgagctcgt cgggggagac ctcggggggg
      421 ccgaagaggg tgagctggcc gcggcgcggg cggtcggggt gcgcggcgag cgcgaagccg
      481 gtgacggggg cgccgggcgg gcgcgcgtcg aggctctggc ggaggaggga gaggagcgtc
      541 ttcgcgtcgc gggtggggcc ggggaggtcg atcgcgcgga cgtcgcaccc ctcgggctcg
      601 aggtggagcg agagctcgag gcgggcgcag ccgagggcct cggcggcgag gcggcggacg
      661 aggcgctcga gggcggcgag agcgaaggcg aggaagggct cgagggcgac gacgggccag
      721 tcgagcgagg ccccctcggt gaaggcgagg gcggggaggc gcgggacgaa gggctcgggg
      781 tcgaggccgc gggccgcctc gtggaggcgg cggccgggct cgccgaggcg ggtgccgacc
      841 tcggcggcgg ggaggcgcgc gagctcgccc gcggtgcgga cgccccagcg ggagagggcg
      901 acggagaggg agagctcggg ggcgagccgc tcgacgggga gcggggcgag gaaacgggcc
      961 tcctcgccgg gggggacgac gacgggggtg cgcgggaggc ccgaggcgat gcgggcggcg
     1021 cgcttgctgg aggcgacgcc ggcgcgggcg gggaggccgt ccttgtcggc ggcgcggagg
     1081 aggtcgaggg cgaggtcgcg ctcgaaggcg agggggtccg gggtggcccg gggcgcgagg
     1141 ccggcgaggt cgaggaaggc gaggccgtcg cccgcgtcct cgacgcgggg cgagaagcgg
     1201 ccggccacgt cgaggagcgc ctcctgcgcg gcgctctcgg cgtgcgggtc gcggccgcgg
     1261 gcgaggaggc gcgggacgag ggcgcgggcc tgcgggaggg tcatgccggg cttgacgccc
     1321 gcggctcggg cggaacgggt ggcggcgacg acgcgcgcgg ccgagccgtt cccctcggtc
     1381 acggcgaggg cctcgtcgcg gagggctggc tcggcgcgga gccgggcggc gagggggaag
     1441 agggggacgg cgagggcgag gaggcggggc gaggccacgg aacgctcctc acgtcctcac
     1501 gcgcaggggg cgaggtggcg ctgagaggga gtggcgggag acggggaggg cccggcgaag
     1561 ggagacggga cggcgagaga ggaggcggcc ggaacggaag gagcagcagc cgtgcgggcg
     1621 aggtggagcg aggggggaga gagcagcgag ggagcgcccg cgggagaggc ggaaggagat
     1681 gacgtcggcg cggtgcgagg ggtgccggcc acggcgcgcg cgggagccgg gggggacgcg
     1741 atttccggga ggagccgctc ggcgagcggg gcggcgaggg gggagagggc gaggggcggg
     1801 gcctcggcgg ggcgacctcc gcgaaccttg aggagcgaga cgcgcgcggc ggcgccggcg
     1861 aggaagcgga cggagccggc ggagcggaag aggccgcggg ccgagacgcc gacgagcgcc
     1921 tcggcggcga ggccggcgag ggggacgggg ctcgagagga ggaaggcgac gcggcgcggc
     1981 gcggcggcgc gggcgaggcg gagccagggg gcggcatcgc gctggcgcga ggaggggagg
     2041 gcgaggccgg cgtcgaggac gacgaggcgg aagccggcgg cggcgacggt ctcggcggcg
     2101 aggagggcgt cgcggaagcg ctgcgggcgg acccagagga ggcgcgcgag gtcgcacccc
     2161 gcctcctcgg cggaggaggg gtcgaggtgg tcgccgaggt cgaggagggc ggccgtctcg
     2221 ccgcgggagg tcgcggcggc gagtgtggcg aggacgaggg cgaagcggcc gccggagcgg
     2281 ccggtcagct ccacgagcgc cccgcgcggg aggccgccgt cgaggagggc gtcgagcggg
     2341 gcgacgccgg tggcgaggcg gtcgccggcg cggagcgcgg cgccgcggcg cgggaggtcg
     2401 gccgcgcgga agaggcggct cgcgacgtcg gcggggaggg ggacggggag agacgacatc
     2461 ctgggaaaag agtgggcgta aatgaggcgt aagtcaagga tggaccggac gggacgccgt
     2521 gctcgcggtg cgaggcctgc cggagggggg cggacggggc cggcggccgt ccaccggtcg
     2581 atgaatgacg gctcattttt cgtgccgcag cgcggcgccg cgtgataccg tccccgaggt
     2641 cgcccgtatc ccgaggcccc aaaaccgcga gaggaaagaa cggatgaatc acgtcgtctg
     2701 catcgcatac gttccggaca ccgagacgaa gatcaagatc ggcgccgacg ggaagtcgat
     2761 cgacgaagcc gacgtcaagt ggatcgtctc cccctacgac gagtacgccc tcgagggggc
     2821 gatcaaggcg aaggaggaga aggggggcac cgtcacggtc gtgacgtacg ggccggcgcg
     2881 ggccgacgtg gggctgcgcg agtgcctcgc gcgcggggcc gacgaggccg tccgcgtcgc
     2941 ctccgacggt ctcggcgagg ccgactcgct cggcgtggcg atggcgctcg ccggggcgat
     3001 caaggcgctc ccgtgcgacg tcctctggct cggcaacaag ggcgtcggga ccgacgcgca
     3061 gacgatggtg ccgatgctcg ccgagctcct cgacctgccg cacgcgaacc tcgtgacgaa
     3121 gctcgagtgg aaggacggcg gcgtcgtcgc ctaccgcgag atcgaggggg cgcgcgaggt
     3181 cgtcgagatg ccgctcccgt gcgtcatcgg cgcgcagaag gggctgaacg agccgcgcta
     3241 cgcctcgctg aaggggatca tggcggcgaa gaagaagacg atcgccgtga agcagccggc
     3301 cgaggtcggc gtcgaccccg cggcgacgac gggcgagaag gccgccgtcc gctggacgaa
     3361 gctggagctg ccgccggccc gccaggccgt gaagctgatc ccggcggacg atccggcgaa
     3421 ggccgccgac gagctggtcc gcctcctccg cgacgaggcg aaggtcctct aggccgacgg
     3481 cgagacaagg aagagacacg atgagcaacg tcctcgtatt catcgagcag cgcgacggca
     3541 agatccgcaa ggcctccttc gaggccctca ccctcggccg cgaggtcgcg gcgaagacag
     3601 gcggcgccct cggcgccgtc gtcgccgggt ccggcatcgg ggggctcgcc cccgagctcg
     3661 ccgcctacgg cgccgcgaag gtcttcgtcg ccgacaagcc cgagctcgcc ctctattcct
     3721 gcgaggggta cacggcggcc ctcgacgcgg cggtcgcggc gttcgcgccg gccgtcgtcc
     3781 tcgtcccggc cacgtccatg ggcaaggacg tcgccccgcg gctcgccgcg cggcggaagg
     3841 tcggcctcct ctccgacgtg atgtccctcg aggtcgaggg aggccgcctc gtggcgaccc
     3901 gctcggtcta ctcgggcaag gcgcgcgcga cggtcgcggc cgatcccggg gcggcgctcc
     3961 agatcgccac cccccgcccg aacgtcttcc cggcgtcgaa gggcgcggcg ggcgcgggcg
     4021 aggtcgtcgc gctcgacttc tccgtgacga tccgcgcgaa ggtcgtgaag ctggagatgc
     4081 cggaggcggg cgagctcgac gtggccgagg ccgacaagat cgtctccggc ggccgcggca
     4141 tcaaggggcc ggaaggctgg ccggtcctcc gcgcgctctg ccacgagctg ggcgccgcgc
     4201 tcggcgcctc gcgcgccgcg gtcgacgcgg ggtggatcgg gcacgagcac caggtcgggc
     4261 agacgggcaa ggtcgtctcg ccgacgctct acgtcgcctg cggcatctcg ggcgccatcc
     4321 agcacatggc gggcatgggc tcctcgaagg tgatcgtggc gatcaacaag gacgccgacg
     4381 cgccgatctt caaggccgcg acgtacggca tcgtgggcga cctgttccag atcgtcccgg
     4441 cgctgaccga agctgtccgg aaggcgaagg cgtaggcagc gttgccgtgg gtccgggggc
     4501 cgcgtgcggc ccccggattt cccgacgaaa cgaaaagggc ggccgcgagg ccgcccttca
     4561 cgtttccgga tggttccgga gcgctacggc tcgggagtct ctcccgtcac gttcgcggtg
     4621 aagtcggtgt agccgatgcc gcagccgttc acccagagga tccggatgat gtcgatcccg
     4681 gtgtacgggc cggcccggta gatgaaccgc cccgggacgt tcgggactcg cgagaacatc
     4741 ccgcggctct gggactgcac gacggcgaag cggacgtcgt cgagcaaggg gaccgaggcc
     4801 gggttgacgt cgatgatggt ctgcgtccct ccggcgacgg ccacggcgcc cggcgtgatc
     4861 gtcgggacgt agagctgcgg gtcgatcggg acggtgacgg tggccgtcgt ctcggcgccg
     4921 tcgaggctgt aggccgtgag gacgaacgag gccgtcgcgg tgagcgtcac ctcgacgccg
     4981 ccgttcgcga cgaagttctc gtccagcccg ctgatgtaga cgccgatggc gttctcggtc
     5041 cgccaggtga ggaggaagtt cgtcgagggg gcgcagccgc gctccgggat cgcctcgaag
     5101 ctgaggatcc gcggcgcgcc ggggaggacg acgtgggccg tgaactccgc gctcgcgccg
     5161 ccgcaggagc cggtcgccgt gatcttcacg acgtcggtgc ccggggtcgt tcccgcggtg
     5221 tagatgaagg aggtcccctg gacggtgaag gtggagcccg tcgactcgcg ggagacgaac
     5281 tcgacggtga ccggaccggt cccgacgggg aggatgccgg tgacgacgcc gctcgtcggg
     5341 agggtggccg aggcgggcgc cggggtcggc gccggcgtcg acggcgtgat cgtgatccgg
     5401 aggggctgga tcgtcgactg cccggtggag gaggtggccg tcaggatgaa gtcgccggtg
     5461 gcgtggacgg tggtcttcac ggagccggtg gccggaaggt cgttgccgac gccgctgatg
     5521 ctgacgttcg cggcgcccga cgtctcccac gagaaggtcg tctcgagagg ggcgcagccg
     5581 gtggccgggc tcgccgtgaa ggaggcgatc gtcggagcgg tggagaaggc gttgcgcttg
     5641 acggcgagcg ggatcgccgt cccgtcgctc gtctggttgt cgttccggat cgcgtacggg
     5701 tagccctggc cggagaggag gcgcaggcgg agcgcgagac ccgccttgcc ggcggcggcg
     5761 gggatgagcg tcgcgaggga agcctgcacc gcgctgccgg gcgcggcctc gatcgtgccc
     5821 gacccgacga gggcgctcga cgagaagagg gcgacctcga gacggcacgg ctgggaggcg
     5881 gtgtcgacgc agacgacgcc ggcgttcgtc cgggcgtggc cggcgccgct ttcggaggac
     5941 gcctcggcgc cgccgccggt cgcctcgtcc cccggcccga ggagctcgga gggcgcggcc
     6001 gccccgaggc tgacgccgta gcgccccgcg gaggcgtcgt tgtacgtctc cgaccaggcg
     6061 gagacgggga cgtcggaacg gacgcggtag aggaacgcgc ccttctcggc gaccgcctcg
     6121 ggagccgtca cgagcgcgac gccgttcgcc gcgaacgtga agggcaccgt ggcgagcagc
     6181 tttccgccgg ccaggaactc gaccgcgccc accgcggggg acgggccggt gacgtagacg
     6241 accgtgtcga accgggcgcc ctggtttccc gttcccttcg cgcccggccc gccccagagg
     6301 tccgtgccct gcggcgccgc aagcgccgcg agcgccacgg ccggtgtcgc ggcggcgagc
     6361 ccgagcagta cggtgagaac gaggaacgac gatgtctttc tcatcagcct ggctccgatc
     6421 ccgtcggatt cgttgggcct tcgaaactta gccgacatca tacacctcgc gaaaccgtcg
     6481 gcggggctcg ggaacggcag gccggcggtc ccgctcctga gctatccttt cctttgatga
     6541 tgccgaccga agcctcctgg ctcggggtcc cggggaccct cgtcttcgcc ctcgtcctca
     6601 tcggcgcgat cgcggcgttc gcttacacgg cttcccggcg atggcagctc ctgacgatcg
     6661 gaggccccga agacgtccgg tgggaccggc cggtcgaacg gctgaagggg ctcttcgagc
     6721 tcggaatctt ccagaagaag atgtggtggg acggctacgc cgggctctac cacatgctca
     6781 tcttcagcgg gttcgtcgtc ctctcggtga ggaccctctc gctcgtcttc gaggggctct
     6841 tcccgaaggc cgggatgccg ttcctgcccg cgggggcgtg gcaggcgtac ctgctcctga
     6901 aggacgtggt cctcgtcacg acgctcgtcg gcgtcgtcct cgcgctcgga cggcgctacg
     6961 tcttccgcaa ggagcggctc gacccgtcgt tcgacgcggg gctgatcctc gtcctgatcg
     7021 gtttcctgat ggcgacggac cttctcgcgg gggcggcgaa gttcgccctc gtcccggagc
     7081 acgcctccgc gtgggagcct ttcacggcgg gcctttcggg gctgctcgcg ggcgcggcgc
     7141 cggcgacgct ccaggcggtc ttctcgtggt gctggtggct ccacctcgtc gcgatcctcg
     7201 tcttcctgaa ctacctgccg ttcgcgaagc acttccacgt catcacggcg ctcccgaaca
     7261 tcttcctccg aaagctcgac gcccccggaa ggctcccgac ggtcgacatc gagaaggcgg
     7321 ccgaagcgga gaagttcggc gtcgcaaagg tcgaggacct cacctggaag cagcgcctcg
     7381 acctcttcac ctgcacggag tgcgggcgtt gccgcgaggt ctgcccgacg cacctgacgg
     7441 gaaagccgct gacgccgaag ggcttcctga aggacgtccg gtcggcgctc tacaccgtcg
     7501 cgggcgacct cgtcgacacg gcggaagggc gggcggacgt ggccgcccag gcgaggctcg
     7561 aggagaggaa gcccctcatc ggcggctggg tcgacgagga gacgatctgg gcgtgcacga
     7621 cctgccgcta ttgcgagtcg gcctgcccgg tgacgatcac gtataccgac aagatcgtcg
     7681 agatgcgccg ccacctcgtc ctcgagaaga gcgagttccc gaaggaggcg cagacggcgt
     7741 tcaacggcat ggagcgccag gggaacccct ggaacctccc ctccgccgac cgcgacgcct
     7801 ggacgggcga gctcgacttc gaggtcccgg tcctcggggc gatggacgag agcgagaggg
     7861 cgcaggtgga ggttctcttc tgggtcggct gcgccggctc gtacgaggac cgggggaaga
     7921 aggtctcccg tgccctcgcg cggctcctcc acgagggggg cgtgaagttc gcgatcctcg
     7981 gcgcggagga gacctgcaac ggcgactccg cccgccggct cggcaacgag tacctcttcc
     8041 agacgctcgc tcagcagaac gtcgagacga tgaacggcta cggcgtgaag cggatcgtca
     8101 cgaactgccc gcactgcttc aacacgctga agaacgagta ccccgacttc ggcgggacgt
     8161 tcgaggtgat ccacgggacc gagctcgtgg cccttctcct ggccgaaggg cgcctgcagc
     8221 tgacggagag cgtcgcgaag tcgatctcct tccacgacgc ctgctacctc ggccggcaca
     8281 acgacgtcta cgacgcgccg cgggaggtcc tggccgcgat ccccggcctg acgctcacgg
     8341 agctcccgcg gaccgggaag aacggcctct gctgcggcgc cggcggcggg cggatgtggc
     8401 tcgacgagaa gatcgggacc cggatcaacc agctccgcta cgaggagatc gagtcggccg
     8461 ggacggacct cgtcggcgtc tcgtgcccgt tctgcatggt gatgctcggg aacgcccaga
     8521 cgga
//
LOCUS       DAOONL010000134         8400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9927_length_8400_cov_15.968125, whole genome shotgun sequence.
ACCESSION   DAOONL010000134 DAOONL010000000
VERSION     DAOONL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_8400_cov_15.968125"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLP50_14475"
     CDS             complement(<1..766)
                     /locus_tag="PLP50_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPA52798.1"
                     /translation="MRTIEERIRHLDGLRRAARLGGGEARLKKQHEAGKLSARERVEL
                     LGDPGSFEETDPFVVHRCRDFGMDADGNTVPGDGVVAGAVRVEGRPVFVFAQDFTVFG
                     GSLSETNAAKVCKVMDLAMRAGAPVVGLNDSGGARIQEGVASLAGYADIFLRNTLASG
                     VVPQLSAIMGPCAGGAVYSPAITDFVLMVRGTASMFVTGPDVIKSVTHEEVTKEALGG
                     AETHASVSGVAHVVADSDADCILKIRTLLGFLPSNNR"
     gene            complement(783..1061)
                     /locus_tag="PLP50_14480"
     CDS             complement(783..1061)
                     /locus_tag="PLP50_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPA52799.1"
                     /translation="MAAPDARLAACAAFVARRLLDSGLVKAGSGAPVRKAVETVLLFD
                     RDRERQLEAEVEKLLRAHGAATLGAGVDYAEMFRKAKRLLAEKKRIPL"
     gene            1127..2488
                     /locus_tag="PLP50_14485"
     CDS             1127..2488
                     /locus_tag="PLP50_14485"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPA52800.1"
                     /translation="MREPSELARDADDAVRLVSGLLRRGETGEAFRERVRTESWSATE
                     SGLLAPALSVESGTAVRIRREGRLLLVARSGAGPEALRDAVREAGRLAGNAPFFKAHR
                     SSGTSPAERAVERAEAEEEGRSAALAAALARAFPDPRGISATLQLSRIVTERTVVTPR
                     ALLPCGARTRLVAAGTIRRSGAERPFSFQSARPFAEAANALALALHEATRPVPGLPPP
                     PGPVDVVFSPSAASVFWHEVVGHSLEADGGERGSVLSRVKGAAVAPPGLDVHDDPGRA
                     DLPGAYGVDDEGTLARAIPLLADGVVTGLLADRRSGGVESNGHGRTASFRRPPRARMS
                     NLVANAGHASFGELIERCGDGVLVKEVATGSADPESGRFVLLVSAAEAIRRGRRSAPL
                     TPFALGGEILSALGAIEDVWGNEVLPATGLSICVKGGDAVPVGGAAAALLVRGLVART
                     ARR"
     gene            2485..3825
                     /locus_tag="PLP50_14490"
     CDS             2485..3825
                     /locus_tag="PLP50_14490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPA52801.1"
                     /translation="MTPASDDGLFERVLDAAASDGSGAELYERRGRLLELTEDETGTS
                     LLESGEHGLALRLFKGGRSAFAASGPAHALLLPSQAHARLPRARTRRGVRLPAPVAGE
                     APEGRPIGDPPAPDVDLARTLLTGFRQALTSSGRGSVAVREASVALGERTERLATTSG
                     RDTAWGSRLATLVATVVGRTTGGRFSARVVGAAPRPEELPVARLARHAADRVLLPLHG
                     RPFDATRADVLLDPLVASHLVGRLAPLFLGDDEEALLAARTRDGRDALASPLVSIVDD
                     ATVSGGLVRGVRDGEGTPQGRTRLVERGLSSGRLTDTAAALRLSRPLSGNAIRVAWSD
                     APRIGVTNFFVDPSPGVSPLDLLGSVSRGLYAAVLLERPDVDLAADRFRLVVAGYAVE
                     KGRATERISEAEISGRLSEFLRGVSGIGDDLKFAIGGTGSIGSPTLLVPRWRTG"
     gene            complement(3848..4855)
                     /locus_tag="PLP50_14495"
     CDS             complement(3848..4855)
                     /locus_tag="PLP50_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52802.1"
                     /translation="MLAPFPKALLPGAIAVSLLFAPVASGKDVYKPYLDKAIPHHAAI
                     LDTLKKLEKSPEDAQLHNDLGCLVAWDGFWRDALRSFDAASDLDPKDARPWFNAGLVE
                     ALRGEWGAARSRFRKAVKVDPGNWAAWWMLGFAEETLGHENAAVDAYSRSLRVDTSLF
                     DPRVNPFAVATRLKSRVLLRTYERRRVDAALPYSNQLSDTSRIAAFFQARPVAAPVPA
                     PAPVPARTGPVVTAVPSSTVLPDASASEPPPTRSSRRRRAYSRGSDPSEPVELQPEPP
                     RQEPEPAAAESSPERGDAPRTPGPGGGGPGLGSGARRVPGPGGAAPPTEPQPEPKPEP
                     R"
     gene            complement(4858..5556)
                     /locus_tag="PLP50_14500"
     CDS             complement(4858..5556)
                     /locus_tag="PLP50_14500"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HPA52803.1"
                     /translation="MGISIERELIFLERNITALRVEYERFFGGELKRPPVQSRRTIED
                     VLRRAGNAEVEKAVERFRLQSIQSRYNSFVEIWDKRMRAKEEGTTGPVRRLATGAVAP
                     APQATPTPKKASTRKAETAADVAALLAAAEAAVARTTPPGPAPDAPVAPSVKPRSRVD
                     FSPLFKRYVAARQALGEDVTKLRYEKFEEAVRRQAEEIRRRTGSSRLVFEVQTAEGRV
                     KLVGRPVPAPGSKG"
     gene            5580..6989
                     /gene="selA"
                     /locus_tag="PLP50_14505"
     CDS             5580..6989
                     /gene="selA"
                     /locus_tag="PLP50_14505"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPA52804.1"
                     /translation="MARPAVIPPSIEALLSSEEGRALAERHGRLRVRELLREAAARLR
                     AGALEPGADPAAALLAAAEASLAARPPAGPRPVLNATGVLLHTNLGRAPLATAAREAV
                     LRAAGYSTLELDLETGGRGRRGEHVRPLLLALFAPDRPGVDALAVTNAAAALLLALDT
                     LANGRPVAVSRGELVAIGGDFRVPSILARSGASLLEVGTTNKTTPDDYVEALRAGAAA
                     VLKVRPSNYRIVGFTEEVGLPDLAGLCHEARVPLLYDAGAGAPERLEEPGLRDEPVPL
                     EALSGGADLVCFSGDKTLGGPQAGILLGRDDFVRSCARNPLARALRPDKLVLAALAAT
                     LDLHLSGRAAEVPFHAMLATPLPELEARARRLASEAAAAGWEAEAAPLVAIAGGGAGT
                     ESTLPSWGVLLRHPSLGEGDAAARLRARELPVLARVADGRLVLDLRSVSPGEDSALAT
                     ALRETREPWGQREPWGQVL"
     gene            complement(7046..8395)
                     /locus_tag="PLP50_14510"
     CDS             complement(7046..8395)
                     /locus_tag="PLP50_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA52805.1"
                     /translation="MLLVEDRESLRSMLAETLGGEGYRVEAVATGDEAVRRLSEGRRY
                     ALVLTDLKLPGADGLAVLDAALANDPSVPVVLLTGYGTVETAVAAMKRGAADFLNKPV
                     DVDLLLLLVKRHVEARRSAVARALLAEEAGLAGMPRILGRSPALADALERLRRAAPSD
                     VTVLLTGESGTGKELFARALHALSPRRAGPFVAVNVAALPEGLVENELFGHEKGAYTG
                     AGERRAGRFELADGGTLFLDEIGELPLGAQTKLLRAVEERTFLRVGGTVPLSVDVRLV
                     VATNRDLAQRVKAGAFREDLYYRLDVFPVKLPPLRARREDVPELARAFARSSGEGARG
                     RAFEIGAGAAEKLAAWDWPGNVRELKNVVERAVLLAEGPVIRPADVVLGAPGEDAPTT
                     PTLDGTLEETAERWRRAGEAARIRRALEEAGGDRTAAAAALDIPLKRLAQRLRDLRL"
BASE COUNT          997 a         3234 c         3037 g         1132 t
ORIGIN      
        1 gccggttgtt cgagggaagg aagccgagga gcgtccggat cttcaggatg cagtccgcgt
       61 cggagtcggc gacgacgtgg gcgacgccgg agacggaggc gtgcgtctcg gcaccgccga
      121 gcgcctcctt cgtcacctcc tcatgcgtga ccgacttgat gacgtcgggc ccggtcacga
      181 acatcgaggc ggtgccgcgg accatcagga cgaagtcggt gatcgcgggc gagtagacgg
      241 cgccgccggc gcacggaccc atgatcgccg agagctgcgg gacgacgccc gaggcgagcg
      301 tgttgcgcag gaagatgtcg gcgtagcccg cgagggaggc gaccccttcc tggatccgcg
      361 cgcctcccga gtcgttcagg ccgacgacgg gcgccccggc ccgcatcgcg aggtccatca
      421 ccttgcagac cttcgccgcg ttcgtctccg agagggagcc gccgaagacc gtgaagtcct
      481 gcgcgaagac gaagacggga cgtccctcga cgcggacggc gccggcgacg acgccgtcgc
      541 ccggcaccgt gttcccgtcc gcgtccatcc cgaagtcgcg gcagcggtgg acgacgaacg
      601 ggtccgtctc ctcgaaggag cccgggtcgc cgaggagctc gacgcgctcg cgcgccgaga
      661 gcttgccggc ctcatgctgc ttcttcagcc gcgcctctcc cccgccgagc cgcgccgccc
      721 ggcgcagccc gtcgaggtgg cggatcctct cctcgatcgt cctcatcgcg tcgctccgtc
      781 cctcagagcg ggatcctctt cttctcggcg aggagccgct tcgccttgcg gaacatctcc
      841 gcgtagtcga cgcccgcccc gagggtcgcg gcgccgtggg cgcggaggag cttctcgacc
      901 tccgcctcga gctggcgctc gcggtcgcgg tcgaagagga gaacggtctc gaccgccttc
      961 cggacgggcg ctcccgagcc cgccttcacg agtcccgagt cgaggaggcg ccgcgccacg
     1021 aacgcggcgc aggcggcgag tcgggcgtcg ggcgcggcca tggccgcgga ggatacagca
     1081 gcggcccgag cgctccgtcc ggcgactgcc tagaatcggg ccggtgatga gggaaccgtc
     1141 ggagctcgca cgcgacgcgg acgacgccgt gaggctcgtg tcggggctcc ttcgccgcgg
     1201 ggagacggga gaggcgttcc gggagcgcgt ccggacggag agctggtccg ccaccgaatc
     1261 gggcctgctc gcgcctgctc tctccgtgga gagcggcacg gcggtgcgga tccgccgcga
     1321 ggggcgcctc ctcctcgtcg cgcgctccgg cgcgggcccc gaggcgctgc gggacgccgt
     1381 ccgcgaggcg ggacgcctcg ccgggaacgc gccgttcttc aaggcccacc ggtcgtccgg
     1441 gacgtctccg gcggaaaggg ccgtcgagcg ggcggaggcg gaggaggagg gacgctccgc
     1501 tgcgctcgcc gccgcgctcg cgcgcgcctt ccccgatccg cgcgggatca gcgccacgct
     1561 ccagctctcg cggatcgtga cggagaggac ggtcgtgacg ccgcgggcgc tcctcccctg
     1621 cggcgcgcgg acgcgcctcg tcgccgccgg cacgatccgc cggtccggcg ccgaacgtcc
     1681 cttctccttc cagtcggcgc ggccgttcgc cgaggcggcg aacgccctcg ccctcgcgct
     1741 ccacgaagca acccgtccgg tccccggcct tccgccgccg ccgggcccgg tcgacgtcgt
     1801 cttctcgccc tcggccgcgt ccgtgttctg gcacgaggtc gtcggacact cgctcgaggc
     1861 cgacggcggc gagcgcggct cggtcctctc ccgcgtgaag ggggccgcgg tcgctccccc
     1921 cggcctcgac gtccacgacg acccgggccg cgccgacctc ccgggagcgt acggcgtcga
     1981 cgacgagggc accctcgccc gcgccatccc tctcctcgcc gacggggtcg tcaccggcct
     2041 cctcgccgac cgccgctcgg gaggcgtcga gtcgaacgga cacggccgca ccgcgagctt
     2101 ccggcgccct ccacgcgccc ggatgtcgaa cctcgtcgcg aacgccggcc acgcgtcctt
     2161 cggcgagctg atcgagcgct gcggagacgg ggtcctcgtg aaggaggtgg cgaccggctc
     2221 ggccgacccg gaatcggggc ggttcgtcct cctggtctcg gcggccgagg cgatccgccg
     2281 cggccgccgg agcgcccctc tcaccccgtt cgcgctcggc ggggagatcc tctccgctct
     2341 cggcgcgatc gaggacgtct gggggaacga ggtccttccg gccaccgggc tctcgatctg
     2401 cgtgaagggg ggcgacgccg tcccggtggg cggggccgcg gcggccctgc tcgtgcgcgg
     2461 cctcgtcgcg aggacggcgc ggagatgacc cccgcgtccg acgacggcct cttcgagagg
     2521 gtcctcgacg cggccgcttc cgacggcagc ggagcggagc tctacgagcg tcgcggcagg
     2581 ctcctcgagc tgaccgagga cgagaccggc acctctctcc tggagtctgg cgagcacggg
     2641 ctggcgcttc ggcttttcaa aggaggacgt tccgcgttcg ccgcctccgg ccccgcgcac
     2701 gccctcctgc ttccctctca ggcgcacgcg cgcctgccgc gggcccgaac gcgccgcggc
     2761 gtgaggctgc ccgcgccggt cgccggagag gcacccgagg gacgtccgat cggcgatccg
     2821 ccggcgccgg acgtcgacct cgcccggacg ctcctcaccg gcttccgcca ggcgctcacg
     2881 agctccggcc gcggctccgt cgcggtccgg gaagcctccg tcgcgctcgg agagcggacg
     2941 gaacgcctcg cgacgacgtc ggggcgtgac acggcctggg ggagccgcct cgcgacgctc
     3001 gtcgcgacgg tggtcggccg gacgacggga gggcgcttct cggcccgcgt cgtcggcgcc
     3061 gcgccgagac cggaagagct tccggtcgcg cggctcgcga ggcacgccgc cgaccgggtc
     3121 ctcctcccgc tccacggccg tccgttcgac gcgacgcgag ccgacgtcct cctcgacccg
     3181 ctcgtcgcct cgcacctcgt cggacggctc gcgccgctct tcttgggcga cgacgaggag
     3241 gcgcttctcg ccgcccgcac gcgcgacgga cgcgacgcgc tcgcctcccc gctcgtctcg
     3301 atcgtcgacg acgcgacggt ctcggggggc ctcgtgcgcg gagtccgcga cggcgagggg
     3361 actccccagg gccggacgcg gctcgtcgag cgcggcctct cctcgggacg cctcaccgac
     3421 accgccgcgg cgctccggct ctcgcgcccg ctctcgggca acgccatccg cgtcgcctgg
     3481 agcgacgcgc cgcggatcgg cgtgacgaac ttcttcgtcg acccgtcacc gggcgtctcg
     3541 ccgctcgacc tcctcggcag cgtctcccgg ggcctctatg ccgcggtcct cctcgagcgg
     3601 cccgacgtcg acctcgcggc cgaccggttc cgcctcgtcg tcgcgggcta tgcggtcgag
     3661 aaggggcgcg ccacggagcg gatctccgag gcggagatct ccggccggct ctcggagttc
     3721 cttcgcggcg tctccggtat cggcgacgac ctcaagttcg ccattggcgg gaccgggagc
     3781 atcggctcgc cgacgctcct cgtcccgcgc tggaggaccg gctgacgccg ggagggcggc
     3841 gagccgccta cctgggctcg ggcttcggct ccggctgagg ttccgtcggt ggcgccgccc
     3901 cgccgggccc tggaacgcgc cgggccccgc ttccgagccc tggtccgccg ccgcccggtc
     3961 cgggcgtgcg gggcgcgtcg ccgcgctcgg gactcgactc ggcggcggcg ggctccggct
     4021 cctggcgcgg cggctcgggc tgcagctcca ccggctccga tgggtcgctt cccctcgagt
     4081 acgcgcggcg gcggcgcgac gaccgcgtcg gcggcggttc cgacgccgac gcgtccggca
     4141 ggaccgtcga cgacgggacc gccgtcacga ccgggcccgt ccgggccggg acgggagccg
     4201 gcgcggggac cggcgccgcg accggccgcg cctggaagaa ggcggcgatg cgggacgtgt
     4261 cggagagctg gttcgagtac gggagggcgg cgtcgacccg gcggcgctcg taggtcctga
     4321 ggaggacccg cgacttcagc cgggtcgcca cggcaaacgg gttcacgcgc gggtcgaaga
     4381 gcgacgtgtc gacgcgcagc gagcggctgt aagcatccac ggccgcgttc tcgtggccga
     4441 gcgtctcctc ggcgaagccg agcatccacc aggccgccca gttgcccggg tcgaccttca
     4501 cggccttccg gaagcgcgac cgcgccgcgc cccactcccc ccggagcgcc tcgaccaggc
     4561 ccgcgttgaa ccaggggcgc gcgtccttcg ggtcgaggtc ggaggccgca tcgaagctcc
     4621 ggagggcgtc ccgccagaag ccgtcccacg ccacgaggca gccgaggtcg ttgtggagct
     4681 gagcgtcctc ggggctcttc tcgagcttct tgagggtgtc gaggatcgcc gcgtggtgcg
     4741 ggattgcctt gtcgagatac ggcttgtaga cgtccttccc cgacgcgacg ggcgcgaaga
     4801 ggagggagac ggcgatcgcg cccgggagga gcgccttcgg aaacggtgcg agcatcttca
     4861 ccccttcgac ccgggagccg ggaccggccg accgacgagc ttcacccgtc cttccgccgt
     4921 ctggacctcg aagacgagcc gcgacgagcc ggtccgcctg cggatctcct cggcctgccg
     4981 ccgcaccgcc tcctcgaact tctcgtatcg cagcttcgtc acgtcctctc ccaaggcctg
     5041 ccgggccgcc acgtacctct tgaagagggg cgagaaatcg acccggctcc tcggttttac
     5101 ggaaggcgcg accggagcgt cgggggcggg accgggaggg gtcgtccggg cgacggcggc
     5161 ctcggccgcg gcgaggaggg ccgccacgtc ggcggcggtc tccgccttcc tcgtcgaggc
     5221 cttcttcggc gtgggcgtcg cctggggggc cggcgcgacc gcgcccgtcg cgaggcgccg
     5281 aaccggcccc gtcgtcccct cctccttcgc cctcatccgc ttgtcccaga tctcgacgaa
     5341 ggagttgtag cggctctgga tcgactgcag ccggaaccgt tccacggcct tctcgacctc
     5401 ggcgttgccg gcccggcgga ggacgtcttc gatcgtcctt cgggactgga cgggaggccg
     5461 cttcagctcc ccgccgaaga accgctcgta ctcgacccgg agggccgtga tgttccgctc
     5521 gaggaagatc agctcccgtt cgatcgagat ccccatcggt gcgttagatt ctaagggtct
     5581 tggcgagacc ggccgtcatt cctccgtcga tcgaggcgct cctctcgagc gaggaggggc
     5641 gggcccttgc cgaacggcac gggcggctgc gggtccggga gctcctccgg gaggccgcgg
     5701 cccgcctccg ggcgggggcg ctcgagccgg gtgccgaccc ggccgccgct cttctcgccg
     5761 cggcggaagc gtccctcgcg gcccgtcctc ccgccgggcc acgccccgtc ctgaacgcca
     5821 cgggcgtcct tctccacacg aacctcgggc gggcgccgct cgcgacggcc gcccgcgagg
     5881 cggtcctgcg cgccgccggc tactccaccc tcgagctcga cctcgaaacc ggaggtcgcg
     5941 gaaggcgcgg cgagcacgtc cgaccactcc tcctcgccct cttcgccccg gaccggcccg
     6001 gagtcgacgc cctcgccgtg acgaacgcgg ccgcggccct cctcctcgcc ctcgacaccc
     6061 tcgcgaacgg ccggcctgtc gccgtctccc gcggcgagct cgtcgccatc gggggcgact
     6121 tccgggtccc ctcgatcctc gccaggagcg gcgcgtcgct cctcgaggtc ggcacgacga
     6181 acaagacgac gccggacgac tacgtcgagg cccttcgcgc cggggcggcg gcggtcctga
     6241 aggtgcgccc ctccaactac aggatcgtcg gcttcacgga ggaggtcggg ctccccgacc
     6301 tcgcgggcct ctgccacgag gcgagggttc cgctcctgta cgacgccggc gccggcgcgc
     6361 cggagaggct cgaggagccg gggctgcgcg acgagccggt ccctctcgag gcgctctccg
     6421 gcggcgccga cctcgtctgc ttctcggggg acaagacgct cggaggcccg caggcgggga
     6481 tcctcctcgg ccgcgacgac ttcgtccgga gctgcgcgag gaacccgctc gcgcgcgcgc
     6541 tccgccccga caagctcgtc ctcgccgctt tggcggcgac gctcgacctc cacctctcgg
     6601 gccgcgcggc cgaggtgccg ttccacgcga tgctcgcgac gccgctcccg gagctcgagg
     6661 cgcgcgcgcg gcgtctcgcg tcggaagcgg ccgccgccgg gtgggaggcc gaggccgcgc
     6721 cgctcgtcgc catcgccggc ggcggcgccg gaacggagtc gaccctcccg tcctggggcg
     6781 tcctcctccg gcacccgtcc ctcggcgaag gggacgcggc cgcccgcctc cgcgcccgcg
     6841 agctccccgt cctcgcccgc gtcgccgacg gtcgcctcgt cctcgacctc cgcagcgttt
     6901 cccccggcga ggactccgcc ctcgccaccg ccctccggga aacccgggaa ccctggggtc
     6961 agcgggaacc ctggggtcag gtcttgtaat gaggcattcg cgaatgcctc attacaagac
     7021 ctgaccccag gctactgacc ccaggctaga gcctcaggtc tcggaggcgc tgggcgaggc
     7081 gcttgagcgg gatgtcgagg gcggcggcgg cggcggtccg gtcgcctccg gcctcctcga
     7141 gggcgcggcg gatgcgtgcc gcctcgcctg cgcgcctcca gcgctcggcc gtctcctcga
     7201 gcgtcccgtc gagcgtcggc gtggtcgggg cgtcctcgcc gggagcgccg aggacgacgt
     7261 cggccggccg gatgacgggc ccctcagcga ggaggacggc ccgctcgacg acgttcttca
     7321 gctcccgcac gttccccggc cagtcccacg ccgcgagctt ctcggccgcc ccggcgccga
     7381 tctcgaacgc ccgcccgcgc gccccctcgc cgctgctccg cgcgaacgcg cgcgccagct
     7441 cggggacgtc ctccctgcgg gcgcgcagcg gcggcagctt caccgggaag acgtcgaggc
     7501 ggtagtagag gtcctcgcgg aacgcccccg ccttcacccg ctgggcgagg tcccggttcg
     7561 tcgcgacgac gagcctcacg tcgacggaga gcgggacggt cccgccgacg cggaggaacg
     7621 tccgctcctc gaccgcccgc agcagcttcg tctgcgcgcc gagcggcagc tcgccgatct
     7681 cgtcgaggaa gagcgtccct ccgtcggcca gctcgaaacg cccggcgcgc cgctccccgg
     7741 cgcccgtgta ggcccccttc tcgtggccga agagctcgtt ctcgacgagc ccctccggga
     7801 gcgcggcgac gttcacggcg acgaacggcc cggcgcggcg cggcgagagg gcgtggagcg
     7861 ctcgggcgaa gagctccttt cccgtccccg actcccccgt caggagaacc gtcacgtcgc
     7921 tcggagcggc gcggcggagc cgctcgagag cgtcggcgag agcgggcgag cgtcccagga
     7981 tgcgcggcat cccggcgagc cccgcctcct cggcgaggag cgcgcgggcc acggcgctcc
     8041 ggcgcgcctc gacgtgccgc ttcacgagga ggagcaggag gtcgacgtcg accggcttgt
     8101 tcaggaaatc cgccgcgccc cttttcatcg ccgcgacggc cgtctccacc gtcccgtagc
     8161 cggtgaggag gacgaccggg acggatgggt cattcgcgag agcggcgtcc agcacggcga
     8221 ggccgtccgc cccggggagc ttcaggtccg tcaggacgag cgcgtagcgt cgtccttcgg
     8281 agagccggcg gaccgcctcg tcgccggtgg cgaccgcttc gacgcgatat ccctctccgc
     8341 cgagggtctc ggcgagcatc gaccggagcg actcccggtc ctcgacgagg aggatctcgg
//
LOCUS       DAOONL010000135         8378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9961_length_8378_cov_14.944371, whole genome shotgun sequence.
ACCESSION   DAOONL010000135 DAOONL010000000
VERSION     DAOONL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9961_length_8378_cov_14.944371"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="PLP50_14515"
     CDS             <1..2811
                     /locus_tag="PLP50_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPA52806.1"
                     /translation="AFEPLRVKQRQVEFAWGPAGGGAPSRIGSIEVTVTAGKGGKGWL
                     EVADLVLLELPPEPPARPPTWAASSSAPGHEAALAADGDPATSWRAARAGPAEWRVDL
                     GALRELGGLVVEHGGAYASRYGVDLSEDGATWRTVREVASGNGGRDLLALPESEARAI
                     RLRLLEGPRPEPYELKEVSVKPLAFAATPNAFLAEVAKESPRGSWPRYLAGEQSYWTV
                     VGVDGDPDEGLLSEDGALEAGAGLFSIEPFLIAGGRLLTWADVTSRPALVDGDLPIPS
                     VTWDAGETRLEVTALATGLPGASALLARYRVANLTKEERKVRLVLAIRPLQVNPPWQF
                     LGTPGGASEIRSLAFEGARARVNGERVVAALVPPSATGATTLDAGDVSNHLCAGSFPP
                     APAVSDPTGFASGAFAFDLVVPGGSSREVVLAIPFDRSRVPTELAAALGTEPWPGLPP
                     AEASALFESLLARERTAWRSRLDRVAFDVPPEAERWVKVLRSQLAFVLVNRDGPAIQP
                     GSRSYARSWIRDGSLTSEALLRLGQGGVARSFLMWFAGYQGEDGRVPCCVDARGADGV
                     PENDSHGQLLFLIGETVRYTRDRGLAARMWPHVVSAVSYIDALRQKRRTPEYETNEKR
                     VFYGLLPESISHEGYSAKPMHSYWDGFFGLRGLKDAVFLATFLGKDEEAKAFAEIRDE
                     LAADLHASIRLVMERHGIDYVPGCAELGDFDATSTTVALSPGGAEARLPRAALGRTFA
                     KYVENLAKREAGEGDSYTPYEWRNVGALVRLGRRDEALRVAESLLRDLRPAAWNQWGE
                     VVWRDPRTPKFVGDMPHTWVGSDFARSFLDLFAYEREEDSSLVVGAGLPERWIRSERG
                     VSVRGLGTPYGLLDLSLAAEGTDVRVRLSGLRELPPGGLVLKPPLPAIPRTATVDGRP
                     LPLSPAGEVVVRTLPAEVVVRP"
     gene            2852..5248
                     /locus_tag="PLP50_14520"
     CDS             2852..5248
                     /locus_tag="PLP50_14520"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HPA52807.1"
                     /translation="MSQPPARRVDAANAYGRFGDDGLEYVVTDPRTPRPWVNVIANRR
                     AGLVVSQTGSGFTWVDNSQLAVVTRWEQDLTRDSSGRFLWLRDVETGESWPLAPAPSR
                     PVYDRFECRHGIGSTTFRVALHGVGSEWTLFVDPEATAELWRLRLTNESGAPRRLELV
                     AFVEWNCGVTPSPRREFTKLFLECERDAERNAVFARSHMWEVGSARWGHWNTSFPYVS
                     AFACTIRVAEAEGDKAAFLGRLGSFAAPAALSGAPWPGLFGRHGDAVGALRVPVELGA
                     GGSLDLGFAIATAETEEGVRALLARFGAVAAFDASLEAAREEWRRLLASHRVSTPDAT
                     FDAFLNDWVRYQAISARIQGRCGYYQQSGAYGFRDQLQDSQVWLTIDPARCREQVALH
                     AAHQFADGSVYHWWHPLSELGHVTKMTDDLLWLPFVAAAYVKETGDLSVLADRAPFLD
                     DPQPAPLTEHVLRAFERVSRRTSPRGLPYIGAGDWNDGLSAAGLEEMGESVWLAHFLA
                     GLLADWSELWRRTGEPARAADFARRREALVAAINEHAWDGEWYWRATLDSGEKLGSRE
                     SARGSIYLNAQTWAVLNDVAPPGRAAAAMAAVKERLVSDAGALLLAPAYDRPVREIGY
                     ITRYAPGMRENGGVYTHAATWAIAAAAKSKDPELVGRLLQAIDPTRKEPERYLAEPYV
                     LPGNVDGPDSPLHGRAGWTWYTGSAQWLHRVVCEWVVGVRPEWDGMRVDPCLPPGWDE
                     ARMTRPWRGATLEVAVVRDAALAPGEVRVAVDGRRIEGTLLPPVPAGATVRVAVSFRP
                     "
     gene            complement(5279..6631)
                     /locus_tag="PLP50_14525"
     CDS             complement(5279..6631)
                     /locus_tag="PLP50_14525"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPA52808.1"
                     /translation="MSESAPFPEGFLWGAATSAYQVEGSPLADGAGPSIWHRFARIPG
                     KVRDGDTGDVACDHYNRYAEDVALMKGLGLGAYRFSIAWPRVMPEGRGRVNEKGLDFY
                     RRLVEALREAGIRPMATLYHWDLPAALDDRGGWTNPDVASWFADYATACYRALDGAVD
                     LWSTLNEPWVVADGGYLHGVLAPGIASPWAAPRAAKNLLLAHAAGVAAYRAEGKGSIG
                     LVVNLEPKYPASERPEDLAAVARADAYMNRQYLDPALLGKVPEGLAGIFGEAWEGPTD
                     AELAAMKAPIDFLGVNYYTRGVVYDDPRSFPVMEGHAFQRNATHTETGWEVWPDGLYD
                     VLTWIRRRYGNLPLYVTENGAAFYDPPVAHGPVVDDPLRVAYLDAHLGAVRRAIADGV
                     DVRGYFAWSLFDNLEWAHGFRKRFGIVHVDFATQKRTPKASARFYSEVIRTNGECLRG
                     "
     gene            6710..7594
                     /locus_tag="PLP50_14530"
     CDS             6710..7594
                     /locus_tag="PLP50_14530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006103958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA52809.1"
                     /translation="MSDVSLLGTGLLGAPIARRLAATGLSVTVWNRTAAKAEALGREG
                     LAVAPTAASALEASPVAILMLADAPAIRAVLAGPGVVAALAGRTVVQMGTIGPDESRA
                     FAAEVAAAGGEWVEAPVLGSIPQADSGTLQVMVGGTQEQLDRLAPLLSRLGQDVRLVG
                     EVGVAAALKLALNQLIGSLTAAFATSLAYVRASGVPTDLFLEVLRPSALYAPTFDKKL
                     GRMLEAEYENPTFPLRLLGKDLRLFEKAARDAGLEPAVVAAVLRTVEAAEARGHAEDD
                     YSALAEGIAALPPVLPRG"
     gene            7606..>8378
                     /locus_tag="PLP50_14535"
     CDS             7606..>8378
                     /locus_tag="PLP50_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPA52810.1"
                     /translation="MSGDGEEKSGDGVPLVVDLDGTLVRSDLLHESAVRAVVRAPRAA
                     LSAVPALIRNDRAALKRRLALAAPIEAETLPLREEVVAVLREEKARGRRLVLATAADE
                     LLARPVAERVGLFDDLLASDGRKNLGGEAKRAELVSRFGEKGFDYVADRRADLPVFRS
                     ARSAVLVGPAVRLRAEVEGAGTPVAALLPDERPQVRTLLSAIRARQWVKNLLVFVPLV
                     TAHVFAPAAIGAAALAFAALSLLASAVYLLNDLADLPADR"
BASE COUNT         1015 a         3057 c         3127 g         1179 t
ORIGIN      
        1 gcgttcgagc cgctccgcgt gaagcagcgg caggtggagt tcgcctgggg ccccgcggga
       61 ggcggcgcgc cgtcgcggat cgggtcgatc gaggtgacgg tgacggccgg gaaggggggc
      121 aaggggtggc tcgaggtggc cgacctcgtc ctcctcgagc ttccgcccga gccgcccgcc
      181 cggccgccga cctgggccgc ctcctcgtcc gccccgggac acgaagccgc gctcgcggcg
      241 gacggagacc cggcgacgtc gtggcgcgcg gcgcgggccg gcccggccga gtggcgggtc
      301 gacctcggcg cgctgcgaga gctgggaggg ctcgtcgtcg agcacggcgg cgcgtacgcg
      361 agccgctacg gcgtggatct ctcggaagac ggcgccacgt ggcggacggt tcgcgaggtg
      421 gcgagcggaa acggcgggcg ggacctcctg gccctccccg agagcgaggc gcgggcgatc
      481 cggcttcgcc tcctcgaagg tccgcggccg gagccgtacg agctgaagga ggtctcggtg
      541 aagccgctcg ccttcgcggc gacgccgaac gcgttcctcg ccgaggtcgc gaaggagtcg
      601 ccccgcggct cctggccgcg gtatctcgcc ggcgagcagt cgtactggac cgtcgtcggc
      661 gtcgacggcg acccggacga ggggctcctc tcggaggacg gcgcgctcga ggcgggcgcc
      721 ggcctcttct cgatcgagcc gttcctgatc gccgggggcc gtcttctcac ctgggccgac
      781 gtgacgtcgc gtcccgccct cgtcgacggc gacctgccga tcccctcggt gacgtgggac
      841 gcgggggaga cgcgcctcga ggtgacggcg ctcgcgacgg ggctcccggg cgcctccgcc
      901 ctcctcgcgc gctaccgggt cgcgaacctg acgaaggagg agcggaaggt gcggctcgtc
      961 ctcgcgatcc ggccgctcca ggtgaacccg ccgtggcagt tcctcgggac gccgggcggg
     1021 gcctccgaga tccggtcgct cgcgttcgag ggggcgcgcg ctcgcgtgaa cggcgagcgg
     1081 gtcgtcgccg cgctcgtgcc gccctcggcg accggggcga cgacgctcga cgcgggcgac
     1141 gtctcgaacc acctctgcgc cggctccttc cctccggcgc ccgccgtgag cgacccgacc
     1201 ggcttcgcct cgggcgcgtt cgcgttcgac ctcgtcgtcc cggggggaag ttcgcgcgag
     1261 gtcgtcctcg cgatcccgtt cgacaggtcg cgcgtgccga ctgagctcgc ggcggcgctc
     1321 gggacggagc cgtggcccgg cctcccgccc gccgaggcct ctgccctctt cgagtcgctc
     1381 ctcgcgcgcg agcgcaccgc ctggaggagc cgcctcgacc gggtcgcctt cgacgtcccg
     1441 cccgaggcgg aacggtgggt gaaggtcctc cgctcccagc tcgcgttcgt cctcgtgaac
     1501 cgcgacggcc cggcgatcca gcccggttcg cgctcctacg cccgttcctg gatccgggac
     1561 ggctcgctca cctcggaggc gctcctccgg ctcggacagg ggggcgtcgc ccgctcgttc
     1621 ctgatgtggt tcgcgggcta ccagggcgag gacgggcgcg tcccgtgctg cgtcgacgcg
     1681 cgcggcgccg acggcgtccc cgagaacgac agccacgggc agctcctctt cctgatcggc
     1741 gagacggtcc gatacacacg cgaccggggc ctcgcggcgc ggatgtggcc gcacgtcgtc
     1801 tccgccgtct cgtacatcga cgcgctccgg cagaagcgcc ggacgccgga gtacgagacg
     1861 aatgagaagc gggtcttcta cggcctcctc cccgagtcga tcagccacga ggggtattcg
     1921 gcgaaaccga tgcactccta ctgggacggc ttcttcggcc tccgcggcct gaaggacgcc
     1981 gtcttcctcg cgacgttcct cgggaaggac gaggaagcga aggcgttcgc cgagatccgc
     2041 gacgagctcg cggccgacct ccacgcctcg atccgcctcg tcatggagag gcacgggatc
     2101 gactacgtgc caggctgcgc cgagctcggc gacttcgacg cgacgtcgac gacggtcgcc
     2161 ctctcgccgg gcggcgcgga ggcgcgtctg ccgcgcgccg cgctcggacg gacgttcgcg
     2221 aagtacgtcg agaacctcgc gaagcgcgag gcgggggagg gggattccta cactccctac
     2281 gagtggcgaa acgtcggcgc cctcgtgcgc ctgggccggc gcgacgaggc gctccgcgtc
     2341 gcggagagcc tccttcgcga cctgcgcccc gccgcgtgga accagtgggg cgaggtcgtc
     2401 tggcgcgacc cgcggactcc gaagttcgtc ggcgacatgc cgcacacctg ggtcggctcc
     2461 gatttcgcgc gctccttcct cgatctcttc gcgtacgagc gggaggagga ctcctccctc
     2521 gtcgtcggcg ccggactccc ggagcggtgg atccgctcgg agcgcggcgt ctcggtgagg
     2581 gggctcggca ccccgtacgg tctcctcgac ctctccctcg cggccgaagg gacggacgtc
     2641 cgcgtccgcc tctcgggcct gcgcgagctc cctcccggcg gcctcgtcct gaagccgccc
     2701 ctccccgcga tcccgcggac ggcgaccgtc gacggccgcc ccctgcctct ctccccggcc
     2761 ggagaggtcg tcgtccgcac ccttcccgcc gaggtcgtcg tccggccgtg acgcgacccc
     2821 gacggcacgc gaacgccctt tcggaggact catgagccag ccccccgcgc gccgcgtcga
     2881 cgcggcgaac gcctacgggc gcttcggcga cgacgggctc gagtacgtcg tcaccgaccc
     2941 gcggacgccc cgcccctggg tgaacgtcat cgcgaaccgc cgcgccggcc tcgtcgtgag
     3001 ccagacgggc tccggcttca cctgggtcga caactcccag ctcgccgtcg tgacgcgctg
     3061 ggagcaggac ctgacgcgcg actcctccgg gcgcttcctc tggctccggg acgtcgagac
     3121 gggggagtcg tggccgctcg ccccggctcc gtcgcgcccc gtctacgacc gcttcgagtg
     3181 ccggcacggg atcggctcca cgacgttccg tgtcgcgctc cacggcgtcg gatcggagtg
     3241 gacgctcttc gtcgacccgg aggcgacggc cgagctctgg cggcttcggc tgacgaacga
     3301 gagcggcgcc ccgcgccgcc tcgagctcgt cgcgttcgtg gagtggaact gcggggtgac
     3361 gccgtcgccg cggcgggagt tcacgaagct cttcctcgag tgcgagcggg acgcggagcg
     3421 gaacgccgtc ttcgcccggt cgcacatgtg ggaggtcggg tcggcccgct gggggcactg
     3481 gaacacctcc ttcccctacg tgagcgcgtt tgcctgcacg atccgcgtcg ccgaggcgga
     3541 gggggacaag gccgccttcc tcggacggct cggctcgttt gccgcgcccg cggcgctttc
     3601 gggcgcccca tggcccggcc tcttcggccg gcacggcgac gccgtcggcg ctctccgcgt
     3661 gccggtggag ctcggcgcgg gaggctcgct cgacctcggc ttcgcgatcg cgacggcgga
     3721 gacggaagag ggcgtgcgcg cgctcctcgc acgcttcggc gccgtcgcgg cgttcgacgc
     3781 ctcgctcgag gcggctcgcg aggagtggcg ccgcctcctc gcctctcacc gtgtcagcac
     3841 gcccgacgcc acgttcgacg ccttcctgaa cgactgggtc cgctaccagg cgatctcggc
     3901 ccggatccag gggcgctgcg gctactacca gcagagcggc gcctacggct tccgcgacca
     3961 gctccaggac tcgcaggtct ggctgacgat cgacccggcg cgctgccggg agcaggtcgc
     4021 gctccacgcg gcgcaccagt tcgccgacgg ctccgtctac cactggtggc acccgctctc
     4081 ggagctgggc cacgtcacga agatgacgga cgacctcctc tggcttccgt tcgtcgccgc
     4141 ggcgtacgtg aaggagacgg gggacctctc ggtcctcgcc gaccgggcgc cgttcctcga
     4201 cgacccgcag ccggcgccgc tgaccgagca cgtcctccgc gccttcgagc gggtctcccg
     4261 gcggacgagc ccgcgcggcc ttccgtacat cggcgcgggg gactggaacg acggcctttc
     4321 cgccgcgggc ctcgaggaga tgggggagtc cgtctggctc gcccacttcc tcgcgggcct
     4381 cctcgccgac tggagcgagc tctggcggcg gacgggggag ccggcccggg cggcagactt
     4441 cgcgcggcgc cgcgaggcgc tcgtcgcggc gatcaacgag cacgcctggg acggcgagtg
     4501 gtactggcgc gcgacgctcg actccggcga gaagctcggg agccgggaga gcgcgcgcgg
     4561 ttcgatctac ctgaacgcgc agacgtgggc cgtcctgaac gacgtcgctc cgccggggcg
     4621 ggccgcggcg gcgatggcgg ccgtgaagga gcggctcgtg agcgatgcgg gcgcgctcct
     4681 cctcgcgccg gcctacgacc gccccgtccg ggagatcggc tacatcaccc gctacgcgcc
     4741 ggggatgcgc gagaacggcg gcgtctacac gcacgcggcg acgtgggcga tcgcggcggc
     4801 ggcgaagtcg aaggacccgg agctcgtcgg gcgcctcctt caggcgatcg acccgacccg
     4861 gaaggagccg gagcgttacc tcgccgagcc gtacgtcctg ccggggaacg tggacggccc
     4921 cgactcgccg ctccacggcc gcgccggctg gacctggtac accggctcgg cgcagtggct
     4981 ccaccgcgtt gtctgtgaat gggtcgtcgg cgttcgcccc gaatgggacg ggatgcgtgt
     5041 cgacccgtgc ctcccgcccg gctgggacga ggcgaggatg acgcgcccgt ggcggggcgc
     5101 gacgctcgag gtcgccgtcg tccgcgacgc ggcgctcgcg ccgggcgagg tgcgcgtcgc
     5161 ggtcgacggg aggcggatcg aggggacgct cctcccgccc gttccggcgg gagcgacggt
     5221 gcgcgtcgcg gtgagcttca gaccgtagcg gggcccgtcc gcgcggggag gccgggcttc
     5281 agccgcggag gcactccccg ttcgtccgga tcacctcgga gtagaagcgg gcgctcgcct
     5341 tgggggtccg cttctgcgtc gcgaagtcga cgtggacgat gccgaaccgc ttccggaatc
     5401 cgtgcgccca ctcgaggttg tcgaagagag accaggcgaa gtagccacgg acgtcgacgc
     5461 cgtcggcgat cgcgcggcgg acggcgccca ggtgcgcgtc gaggtaggcg acccggagcg
     5521 ggtcgtcgac gacagggccg tgggcgaccg gcgggtcgta gaaggccgcg ccgttctcgg
     5581 tgacgtagag cgggaggttc ccgtagcggc gccggatcca cgtcaggacg tcgtagagcc
     5641 cgtcgggcca gacctcccag ccggtctcgg tgtgtgtcgc gttccgctga aaggcgtgcc
     5701 cctccatgac ggggaagctg cgcgggtcgt cgtagacgac gccgcgggtg tagtagttga
     5761 cgccgaggaa gtcgatcggg gccttcatcg ccgcgagctc ggcgtcggtc ggcccctccc
     5821 acgcctcgcc gaagatcccc gcgagcccct cggggacctt cccgaggagc gcgggatcga
     5881 ggtactggcg gttcatgtag gcgtcggccc gggcgacggc ggcgagatcc tcggggcgct
     5941 cggaggcggg gtacttcggc tcgaggttga cgacgaggcc gatcgacccc ttcccttcgg
     6001 cgcggtaggc ggcgacgccg gccgcgtggg cgaggaggag gttcttcgcg gcgcgcggag
     6061 cggcccaggg gctcgcgatg ccgggggcga ggacgccatg gaggtagccg ccgtcggcga
     6121 cgacccacgg ctcgttcagc gtcgaccaga ggtcgacggc gccgtcgagc gcgcgatagc
     6181 aggccgtcgc gtagtcggcg aaccaggagg cgacgtcggg gttcgtccag ccgccgcggt
     6241 cgtcgagcgc cgccgggagg tcccagtgat agagcgtcgc catcgggcgg atgccggcct
     6301 cgcggagcgc ctcgacgagg cgacgataga agtcgagccc cttctcgttg acgcgccctc
     6361 gtccctcggg catgacacgc ggccaggcga tcgagaagcg gtacgcgccg aggccgagcc
     6421 ccttcatcag cgcgacgtcc tcggcatacc ggttgtagtg gtcgcaggcg acgtcgccgg
     6481 tgtcgccgtc cctcaccttg ccggggatcc gggcgaaacg gtgccagatg ctggggccgg
     6541 cgccgtcggc gaggggcgac ccctcgacct ggtacgcgga cgtggcggct ccccagagga
     6601 agccttcggg gaacggggcg ctctcgctca tcgggtctcc tcgcgcgtgg ggacgcacgg
     6661 tctcgcaggg acggttcccg attatccgcg cgcgtcggag aatcgccaga tgagcgacgt
     6721 ctccctcctc ggcacgggac tcctcggcgc cccgatcgcg cggcgcctcg ccgcgacggg
     6781 tctctccgtc acggtctgga accggacggc ggcgaaggcg gaggcgctcg gccgggaggg
     6841 gctcgccgtc gcgccgacag cggcctcggc actcgaagcc tcgcccgtcg cgatcctgat
     6901 gctcgccgac gccccggcca tccgggccgt cctcgccggg cccggcgtcg tcgcggcgct
     6961 cgcggggcgc accgtcgtcc agatgggaac gatcggcccc gacgagagcc gggctttcgc
     7021 ggcggaggtc gcggccgccg gcggcgagtg ggtcgaagcg ccggtcctcg ggagcattcc
     7081 gcaggcggat tcggggactc tccaggtgat ggtcggtggg acgcaggagc agctcgaccg
     7141 gctcgctccg ctcctttccc gcctcggcca ggacgtccgc ctcgtggggg aggtcggcgt
     7201 ggcggccgcg ctgaagctcg cgctgaacca gctcatcggc tccctgaccg cggccttcgc
     7261 cacgagcctc gcgtacgtcc gcgcgtcggg cgttccgacc gacctcttcc tggaggtcct
     7321 ccgtccgagc gccctctacg ccccgacgtt cgacaagaag ctcgggcgga tgctcgaggc
     7381 ggagtacgag aacccgacgt tcccgctccg gctcctcggg aaagacctcc ggctcttcga
     7441 gaaggccgct cgggacgcgg ggctcgagcc ggccgtcgtg gcggccgtcc tccgcaccgt
     7501 cgaagcggca gaggcgcggg gtcacgcgga ggacgactac tccgcgctcg ccgaagggat
     7561 cgcggcgctt cccccggtcc ttccgcgcgg gtaacctcgt tcgacgtgag cggcgacggc
     7621 gaggagaaga gcggagacgg ggtccccctg gtcgtcgacc tcgacgggac actcgttcgg
     7681 tcggacctcc tccacgagtc ggcggtccgc gcggtcgtcc gcgcgccgcg cgctgccctg
     7741 tcggccgttc ccgccctgat tcggaacgat cgcgccgcgc tgaagcggcg cctcgccctg
     7801 gcagcgccga tcgaggcgga gacgctcccg cttcgcgagg aggtcgtggc ggtcctccgg
     7861 gaggagaagg cccgcgggcg gcggctcgtc ctggcgacgg ccgccgacga gctccttgcg
     7921 cggcccgtcg ccgagagggt cgggctcttc gacgacctcc tcgcgagcga cgggcggaag
     7981 aacctcggag gggaggcgaa gcgcgccgag ctcgtctccc gcttcggcga aaagggcttc
     8041 gactacgtcg ccgaccgccg ggccgatctg ccggtcttcc ggagcgctcg gagcgcggtg
     8101 ctcgtcggcc ccgccgttcg gctgcgggcg gaggtcgagg gggcggggac gcctgtcgcc
     8161 gccctcctgc cggacgagag gccgcaggtc cgtacccttc tgtcggcgat ccgcgcgcgg
     8221 cagtgggtca agaacctcct cgtcttcgtc ccgctcgtca cggcgcacgt cttcgcgccg
     8281 gcggcgatcg gggcggcggc gctcgcgttc gcggcgctgt cgctcctcgc ctccgccgtc
     8341 tacctcctga acgacctcgc cgacctgccg gccgaccg
//
LOCUS       DAOONL010000136         8374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_9971_length_8374_cov_16.391754, whole genome shotgun sequence.
ACCESSION   DAOONL010000136 DAOONL010000000
VERSION     DAOONL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_8374_cov_16.391754"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /gene="rplI"
                     /locus_tag="PLP50_14540"
     CDS             complement(<1..198)
                     /gene="rplI"
                     /locus_tag="PLP50_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPA52811.1"
                     /translation="MSDVKVILTDEVRGLGNRGEVVSVAAGYARNFLLPKELAYLATP
                     GNVKRLEQERKRYDVAQAKEKE"
     gene            complement(206..1120)
                     /locus_tag="PLP50_14545"
     CDS             complement(206..1120)
                     /locus_tag="PLP50_14545"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPA52812.1"
                     /translation="MVLPPLAPWLLPSAAARLARAGRELTSPAAAFPLAAAVLPFATF
                     FGLVGGGVGSALSAATALAFVALPALGLVAGATAGRRREELAWLAAALAGIGALGTVL
                     AFTLTEGADPGAILALRLDAARPELLTFYRNAGWSEETLAATGAAFDRLRDLVAWQLP
                     GLLLSACVLHGSLLVYAFGRRAGLDATPLAAGSFATFRTPLAAAAVFVPAGALAALAD
                     GAAARAATTVLIPLGVLFFLRGMAIIRALLDRGRVGLLIRAPVYVLAVQMPIPVLVAI
                     GGLLDEFLDFRGRFPRKEESDGGSGRRE"
     gene            complement(1374..1637)
                     /gene="rpsR"
                     /locus_tag="PLP50_14550"
     CDS             complement(1374..1637)
                     /gene="rpsR"
                     /locus_tag="PLP50_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPA52813.1"
                     /translation="MAAPSGPAARRPAKKKFFVRRKRVCKFTVEKIGYIDFKDVKLLQ
                     SFVPERAKILPRRISGTSAFYQRKLAVAIKRARYLALLPYVAD"
     gene            complement(1652..2155)
                     /gene="rpsF"
                     /locus_tag="PLP50_14555"
     CDS             complement(1652..2155)
                     /gene="rpsF"
                     /locus_tag="PLP50_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPA52814.1"
                     /translation="MTETQKRLYDLVFLIAPDKDEQGAAAVVEEFRKLLTDLGAVIEK
                     DESMGRRRLAYQIKKRGEATYHNFLFRADAKAVAELQRKMKLSESILRFLTVRIDEEL
                     RHGQKVARRTKTRTPRVVPASPESVPAPEAVAAPAPAPAPVVEPASAPAPAPESAAPE
                     APAQPES"
     gene            complement(2224..2841)
                     /gene="pth"
                     /locus_tag="PLP50_14560"
     CDS             complement(2224..2841)
                     /gene="pth"
                     /locus_tag="PLP50_14560"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPA52815.1"
                     /translation="MTELALVVGLGNPGDRYVGTRHNVGWEVLDSLVRRLAADPSRPT
                     RIDRLDCRALTGRVRIGATPVLLAKPQTYMNLSGESVKGLLLKHAVPPERMLVVSDDV
                     ALPVGRLRIRPSGSSGGQKGLQNVIDCLGHDGFPRLRVGIAGDHFRPGEDKADYVLER
                     FSKAERSALAPVVDTACEAIETFVLEGIDAAMNRFNRSPETVPAG"
     gene            complement(2844..3551)
                     /locus_tag="PLP50_14565"
     CDS             complement(2844..3551)
                     /locus_tag="PLP50_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPA52816.1"
                     /translation="MLQDTVVVEVEKRTETGKNACNRLRAKDLIPAVLYGGHSGAESL
                     PLSVPRKDLLALFRKGLHQNAVFRVHVKGADEQPHVMVRDVMLSPLRRELVHLDFVRV
                     LLDRKLKVKVPVETVGTAIGVKLGGLLDVVTRELEVECLPADIPASIQADVSNLDASG
                     VMRVADLALGEKVQVLGDPDRVIAHIVTHKTEEAATADAAPAAEPEVVKKGKKEEEGA
                     AKPAAKAAPKAEKKEKK"
     gene            complement(3765..4721)
                     /locus_tag="PLP50_14570"
     CDS             complement(3765..4721)
                     /locus_tag="PLP50_14570"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPA52817.1"
                     /translation="MTLRVPYGELKVFGGRAHPSLTGEICRALGREAGRVTLYNFSDG
                     ENYCQIEENVRGADVFVVQPTCPPVNANLMELLIMLDAFKRSSASRVTAVLPYYGYAR
                     QDRKDKPRVPITAKLVADLLTSAGADRVLAVDLHAAQIQGYFDIPVDHLFASPVLLDA
                     IRDAKLDPLTVVSPDAGGVERARAFGKRLGVSLAIVDKRRKGKNEVEVMHVIGDVKGR
                     NVLIIDDIIDTAGTLINTVAALKEAGAERVFSACVHPVLSGPAIDRLNASSIERVFCS
                     NSLPVDDKLARCPILTPLSVGPLLGEAIHRIHEGTSVSSLFV"
     gene            complement(4735..4809)
                     /locus_tag="PLP50_14575"
     tRNA            complement(4735..4809)
                     /locus_tag="PLP50_14575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4775..4777),aa:Gln,seq:ttg)
     gene            complement(4825..5685)
                     /locus_tag="PLP50_14580"
     CDS             complement(4825..5685)
                     /locus_tag="PLP50_14580"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPA52818.1"
                     /translation="MRLVAEAFAKVNRSLLVLGRRPDGFHELDTVFQAVGLTDRLVFE
                     ESDALTLRVDDPSVPAGPENLVLRAARLLADEAGVKARAAIDLEKRIPSGGGLGGGSS
                     DAAVTLLGLSALWGLDAPLETLAEVGAGLGSDVPFFLFGGTAHGTGRGERIAPLPDMP
                     AKGVVLVMPPFPAATPEVFRRLGAPAWDGRRSDAGRDLPDRNDLEPAAEALFPPLRAV
                     REALANAGAEQARLSGSGSTVFGLFPDLAAAAEAARRLDGLPVGSAIRVVPTWTREEC
                     RSRSGPRRSE"
     gene            complement(5682..6326)
                     /locus_tag="PLP50_14585"
     CDS             complement(5682..6326)
                     /locus_tag="PLP50_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52819.1"
                     /translation="MTRAVAAAAAALLVLGACRSAGPAGAPAAPQETAQALSSVSAAR
                     EAAWRPRRFQALFRGEVSPKVGIAVRGYLTLRWDGEALAWKASAPLAGAGREGVLRRE
                     GGGAEGLLPGRLASRDVLSALFGVPEESPSGEGASRLRNGRLVLPLASGDGRAVLVSP
                     AGEVVGLLLPDGVRVELSPGAGLPRRIAIRGPEGSAVLSLESFGALPEAGGSNG"
     gene            complement(6323..8272)
                     /locus_tag="PLP50_14590"
     CDS             complement(6323..8272)
                     /locus_tag="PLP50_14590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52820.1"
                     /translation="MRFRPVTAVLALAILAPPVAAAPPVRSEALSPAESARRDAVERF
                     LRARLFAAEGEFQEALKEFRRAVELDPADGHLRREYAEALRDFGILPEAEEQARRAVE
                     LVPGSAAAHRVLGQILLAAARDRGGIEAAIAPLKTANDLQPTEPAGALALGQAYLRLE
                     RFEEAAAVLSRVQQNVRGPLLPLLYGEALEKSGRPEEAEESYLGILRMDEQNAAASFG
                     LLRVYQATRKLDKALPILADLVKRQPGNHALRAQYGLSLLRARRLSEAEAAFQEVLKA
                     DPDHRDALRHYAALLSERLETARAEELLKKLQGLEPDDADVAFRRALNFLEARRLDEA
                     ETVLRDLRSQLVARKGPAGGIVSVDGQLGYVAYLRKDWAAAREAVRPHLLEEDGSVNM
                     QALNLLTLVARDAKEPAQGLQACREAFAKAPKDLDVRGLLAEFLLRSEKEKERKEGEE
                     MIGALAKEGREGALAAADAWQRLERYGRAAETAAAALAAHPDDPDLLFRQAASLEREK
                     KVPESVAAFERLIALKPDHGAALNYLGYMFADRNENLDRALELVTRAVALEPSNAAYL
                     DSLGWIHFRLGNLDEAEKHLLTAKRLSPDDPTIEDHLGDLEERRGDLAKARERWARAL
                     ALEPEDGGAAIRAKLERTAAAAKQP"
BASE COUNT         1169 a         2972 c         3093 g         1140 t
ORIGIN      
        1 ctccttctcc ttcgcctggg ccacgtcgta gcgcttccgc tcctgctcga gccgcttcac
       61 gttgccgggc gtggcgaggt aggcgagctc cttgggaagg aggaagttgc gggcgtagcc
      121 ggccgccacc gagacgacct cgccccggtt gccgaggccg cgcacctcgt cggtcaggat
      181 cactttcacg tcggacatgt tccgcttact cccttcttcc cgacccgccg tcgctctctt
      241 ccttccgggg gaaccgccca cggaagtcga ggaactcgtc gaggagcccg ccgatggcca
      301 cgaggaccgg tatcggcatc tggacggcga ggacgtacac cggcgctcgg atcaggaggc
      361 caacacgccc cctgtcgagc agagcgcgga ttatcgccat cccccggagg aaaaacaaga
      421 cgccgagcgg aatcaggacc gtcgtcgcgg cccgggccgc ggccccgtcg gcgagggcgg
      481 ccagcgcgcc ggccggaacg aagaccgccg cggcggcgag gggggtccgg aaggtcgcga
      541 acgagccggc cgcaagaggc gtcgcgtcga gccccgcccg acggccgaag gcgtagacga
      601 ggagcgaccc gtgcaggaca caggccgaga gaaggagacc cggcagctgc cacgccacga
      661 ggtcgcgaag ccggtcgaac gcggcgcccg tcgccgcgag ggtctcctcg gaccacccgg
      721 cattccggta gaacgtgagg agctcgggcc gggccgcgtc gagccggagg gcgaggatcg
      781 cccccgggtc ggccccctcc gtgagggtga aggccaggac ggttcccaac gccccgattc
      841 cggcgagcgc ggccgcgagc caggcgagct cctcacggcg gcgtccggcg gtcgcgccgg
      901 cgacgagacc gagcgcgggc agcgcgacga acgcgagcgc cgtcgcggcc gagagggccg
      961 agccgactcc gcctccgacg aggccgaaga acgtcgcgaa cggcaggacg gcagccgcga
     1021 gcggaaaagc ggcggccggg ctcgtcagct cgcggccggc ccgcgccagg cgcgccgcgg
     1081 ccgacggcag cagccatggg gcgagcggcg gaaggaccac ggcgagagcc gcagagagac
     1141 ccgccagagg gggggccaga aacggcaagc ccctccggcg cggagccgga ggggcggagc
     1201 cggacggggg ctcaccgggg ggcgcgctct ccggggggcg ctgttcgccg ccccccggtc
     1261 cccccggact catgacgcgg cgctcaccgc tgtgttcagt catggccgcg cgagacgccg
     1321 ccccccggac cccccgggtc cggacagtga caagcgctcc cccagacggg ctcctagtca
     1381 gcgacgtacg gaaggagcgc caggtagcgc gcgcgcttga tggcgacggc gagcttccgc
     1441 tggtagaagg ccgaggtccc gctgatgcgg cgcgggagga tcttggcccg ctcggggacg
     1501 aagctctgga ggagcttcac gtccttgaag tcgatgtagc cgatcttctc gacggtgaac
     1561 ttgcagacgc gcttgcggcg gacgaagaac ttcttcttcg cggggcggcg ggcggcggga
     1621 ccggacggag cagccatcga tctctcctga atcagctctc gggctgggcc ggcgcctcgg
     1681 gggcggcgga ctcgggggcc ggcgcgggcg ccgaggcggg ctcgacgacc ggggccgggg
     1741 caggggccgg ggcggcgacg gcctccggcg cggggacgga ctcgggcgag gcggggacga
     1801 cgcgcggcgt gcgcgtcttc gtccggcgcg ccaccttctg cccgtggcgc agctcctcgt
     1861 cgatgcggac cgtgaggaag cggaggatgc tctcggagag cttcatcttc cgctgcagct
     1921 cggccacggc cttcgcgtcg gcccggaaca ggaagttgtg gtaggtcgcc tcgccccgct
     1981 tcttgatctg gtaggcgagg cgccggcggc ccatcgactc gtccttttcg atgacggcgc
     2041 cgaggtcggt caggagcttg cggaactcct cgacgacggc cgcggctccc tgctcgtcct
     2101 tgtcgggggc gatcaggaaa accaggtcgt agagacgctt ctgggtttcg gtcacttgtc
     2161 ctccttgcgg atcgcggggc gagcccgcga ggcgcggccc ttcggccgag ccaagggttg
     2221 gattcagccc gccggaacgg tctccggcga gcggttgaaa cggttcatgg cggcgtcgat
     2281 cccctcgagg acgaaggtct cgatcgcctc gcaggcggtg tcgacgacgg gcgcgagcgc
     2341 gcttctctcg gccttcgaga accgctcgag gacgtagtcg gccttgtcct ccccgggccg
     2401 gaagtggtcc cccgcgattc cgacccggag gcgcgggaag ccgtcgtgcc cgaggcagtc
     2461 gatgacgttc tggaggccct tctgcccccc ggaggagccc gacgggcgaa tcctcagacg
     2521 ccccaccggg agcgcgacgt cgtccgagac gacgagcatc cgctccggcg gcacggcgtg
     2581 cttgagaaga agccccttca cggattcgcc cgagaggttc atgtacgtct gaggcttcgc
     2641 gaggaggacc ggcgtggcgc cgatcctcac ccgtcccgtc agggcccggc agtcgaggcg
     2701 gtcgatgcgc gtcgggcggg acggatcggc ggcgagccgc cggacgagag agtccagcac
     2761 ctcccagccg acgttgtgcc gcgtcccgac gtagcggtcg cccgggttgc cgagccccac
     2821 gacgagggcg agctcggtca agcctacttc ttctccttct tctcggcctt cggcgcggcc
     2881 ttggccgccg gctttgcggc gccttcctct tccttcttgc ccttcttcac gacctcgggc
     2941 tcggccgccg gggcggcgtc ggccgtcgcg gcctcctcgg tcttgtgggt gacgatgtgc
     3001 gcgatgacgc ggtcggggtc cccgaggacc tggaccttct cgcccagggc gaggtccgcc
     3061 acgcgcatca cgccgctggc gtcgaggttg gagacgtcag cctggatcga ggcggggatg
     3121 tcggccggga ggcactcgac ctcgagctcg cgcgtgacga cgtcgagcag gccgccgagc
     3181 ttgacgccga tcgcggtgcc gacggtctcg accgggacct tcaccttcag cttccggtcc
     3241 agcaggacgc ggacgaagtc gaggtggacg agctcgcggc ggagggggct cagcatcacg
     3301 tcgcggacca tcacgtgcgg ctgctcgtcg gcgcccttga cgtggacgcg gaagacggcg
     3361 ttctggtgca gccccttccg gaagagggcg aggaggtcct tccgggggac gctcaacggg
     3421 agcgactcgg cgccggagtg gccgccgtag aggacggcgg ggatgaggtc cttcgcgcgg
     3481 aggcggttgc aggcgttctt gcccgtctcg gtccgcttct cgacttcgac gacgacggtg
     3541 tcctggagca tttcgatcgt acctttcccc gggcgaacgg tccggcaggt tccgggcgtc
     3601 cggtccggcg cagagccgcg gattctacac gaagcgcccg aatctcaggt ccggggggcc
     3661 cgggggggcc acgaacagcg cccccccggc gagggcacgc tccgctccgc cgcggagggg
     3721 gattgggagg acccgggcgg aggccgggtt cccctgatcc agattcagac gaagagcgag
     3781 gagacggacg ttccctcgtg gatccggtgg atcgcctcgc cgaggagcgg tcccaccgag
     3841 agcggcgtca ggatcgggca gcgggcgagc ttgtcgtcga ccggcagcga gttcgagcag
     3901 aagacgcgct cgatggagct cgcgttcagc cggtcgatcg ccgggcccga caggaccggg
     3961 tggacgcagg ccgagaagac ccgttccgcc cccgcctcct tgagtgccgc caccgtgttg
     4021 atgagcgtcc cggccgtgtc gatgatgtcg tcgatgatca ggacgttccg tcccttcacg
     4081 tcgccgatga cgtgcatcac ctcgacctcg ttcttcccct tccgccgctt gtcgacgatc
     4141 gcgagggaga cgccgagccg cttgccgaac gcgcgggcgc gctccacgcc gcccgcgtcc
     4201 ggcgagacga cggtgagggg gtcgagcttc gcgtcgcgga tcgcgtcgag gaggaccggc
     4261 gaggcgaaga ggtggtcgac cgggatgtcg aagtagccct ggatctgcgc ggcgtgcagg
     4321 tcgaccgcga ggacgcggtc ggcccccgcc gacgtgagga ggtcggcgac gagcttggcg
     4381 gtgatcggga cgcgcggctt gtccttccgg tcctggcggg cgtagccgta gtacggcagg
     4441 accgccgtca cgcgcgacgc ggaggatcgc ttgaacgcgt cgagcatgat gaggagctcc
     4501 atcaggttcg cgttcaccgg gggacacgtc ggctgcacga cgaagacgtc ggctccgcgg
     4561 acgttctcct cgatctggca gtagttctcg ccgtccgaga agttgtagag cgtcacccgc
     4621 cccgcctcgc gtcccagggc ccggcagatc tcgccggtca gggacgggtg cgcacgccct
     4681 ccgaagacct tcagctctcc gtacggaacc ctcagcgtca tggctctctc atcctggctg
     4741 gggagggagg attcgaacct ccgaatggcg gttccaaaga ccgctgactt accacttgtc
     4801 gactccccaa agccgggccg gatattactc tgaccgccgc ggcccgctcc gggagcggca
     4861 ttcctcgcgg gtccacgtcg ggacgacgcg gatcgcggaa cccacgggca ggccgtcgag
     4921 ccttcgtgcg gcctccgcgg ccgccgccag gtcgggaaag aggccgaaga cggtcgaccc
     4981 gctccccgag agccgggcct gctcggcgcc ggcattcgcg agcgcctcgc gcacggcccg
     5041 gagcggcggg aagagcgcct ccgcggccgg ctcgaggtcg ttccggtcgg gcaagtctct
     5101 cccggcgtcg ctccgacggc cgtcccacgc cggcgccccg aggcgccgaa agacctccgg
     5161 cgtcgccgcg ggaaagggcg gcatcacgag aacgactccc ttcgccggca tgtcgggcag
     5221 cggcgcgatc cgctcacccc gtccggtccc gtgggcggtc cccccgaaga ggaagaacgg
     5281 gacgtcggag ccgaggccgg cgccgacctc cgcgagcgtc tccagggggg cgtcgaggcc
     5341 ccagagcgcc gagaggccga ggagcgtcac ggccgcgtcg gagctgccgc ctccgagccc
     5401 cccgccggag gggatcctct tctccaggtc gatcgcggcg cgagccttca ctccggcctc
     5461 gtccgcgagg aggcgagccg cccgaaggac gaggttctcc gggccggcgg ggacggaagg
     5521 gtcgtccacc cggagcgtca gcgcgtcgct ctcctcgaag acgagccggt ccgtcaggcc
     5581 gacggcctga aagacggtgt cgagctcgtg gaagccgtcg ggccgcctgc cgagaacgag
     5641 gagcgagcgg ttcactttcg cgaacgcttc ggcgacgagc ctcatccgtt cgagcctccg
     5701 gcctccggaa gcgcgccgaa gctctcgagc gacagcaccg cgctcccctc gggcccccgg
     5761 atcgcgatgc gacgcgggag gccggcgccg ggcgacagct ccacgcggac gccgtcggga
     5821 aggaggagcc cgacgacctc tcccgcgggc gagacgagga ccgcgcggcc gtcgccggag
     5881 gcgagcggaa ggacgaggcg tccgttccga aggcgcgacg ccccctctcc ggagggggac
     5941 tcttccggga ccccgaagag cgcggaaagg acgtcacgcg atgcgagtcg gcccggcagg
     6001 agcccttcgg ccccgccccc ctcccttcgg agtacgccct cgcgtcccgc cccggcgagc
     6061 ggcgccgagg ccttccaggc gagcgcctct ccgtcccatc tcagcgtgag gtagccgcgg
     6121 accgcgatgc ccaccttcgg agaaacctcg ccgcgaaaga gcgcctggaa ccggcgaggc
     6181 cgccaggccg cctcccgcgc cgcggaaacc gaagacaggg cctgcgccgt ctcctgcggg
     6241 gccgcgggag ctccggcggg cccggccgag cggcacgccc cgaggacgag cagcgcggcc
     6301 gcggcggccg cgaccgcgcg cgtcacggct gcttcgccgc ggcggctgtc ctctcgagct
     6361 tcgcccggat cgcggctcca ccgtcctccg gctcgagggc gagcgcccgc gcccaccgct
     6421 cgcgcgcctt cgcgaggtcg cccctcctct cctcgaggtc cccgaggtgg tcctcgatcg
     6481 tcgggtcgtc cggcgagagg cgcttggcgg tcagcaggtg cttctccgcc tcgtcgaggt
     6541 tgccgagccg gaagtggatc cagccgagcg aatcgaggta cgccgcgttc gacggctcga
     6601 gggcgaccgc ccgggtgacg agctcgagcg cccggtccag gttctcgttc cggtccgcga
     6661 acatgtaccc gaggtagttg agcgcggcgc cgtgatcggg cttcagcgcg atcagcctct
     6721 cgaacgccgc gacggactcg ggcaccttct tctccctctc gagggacgcc gcttgccgga
     6781 agaggaggtc cgggtcgtcc gggtgggccg cgagagccgc cgcggccgtc tcggcggcgc
     6841 gcccgtatcg ttcgagccgc tgccacgcgt cggccgcggc gagggcgccc tcccgcccct
     6901 ccttggcgag ggccccgatc atctcctcgc cttcctttct ctccttctcc ttctcggagc
     6961 ggaggaggaa ctcggcgagg aggccgcgga cgtcgaggtc cttcggcgcc ttcgcgaagg
     7021 cctcccggca ggcctgcagc ccctgcgccg gctccttcgc gtcccgcgcg acgagcgtca
     7081 ggaggttcag ggcctgcatg ttcacggaac cgtcctcctc gaggaggtgc ggccggacgg
     7141 cctcccgcgc cgcggcccag tccttccgga ggtaggcgac gtagccgagc tggccgtcga
     7201 cggagacgat cccgccggcc ggccccttcc gcgcgacgag ctgcgagcgg aggtcccgaa
     7261 ggaccgtctc cgcctcgtcg agccgacgcg cctcgaggaa gttcaaggcg cgccggaacg
     7321 cgacgtccgc gtcgtccggc tcgaggccct gcagcttctt caggagctcc tcggcgcgcg
     7381 ccgtctcgag cctctcggag aggagcgccg catagtggcg cagcgcgtcg cggtggtccg
     7441 ggtcggcctt cagcacctcc tggaacgccg cttcggcctc ggaaaggcgg cgcgcccgaa
     7501 ggagggagag gccgtactgc gcgcgcagcg cgtggttccc cggctgccgc ttcacgaggt
     7561 ccgccaggat cggcagcgcc ttgtcgagct tccgggtcgc ctggtagacg cgcaggaggc
     7621 cgaacgaggc cgccgcgttc tgctcgtcca tccggaggat gccgaggtag gactcctcgg
     7681 cttcttcggg gcggccgctc ttctcgagcg cctcgccgta gaggagcggg aggagcggcc
     7741 cccggacgtt ctgctggacg cgtgagagga cggcggccgc ctcctcgaac cgctcgaggc
     7801 gcaggtaggc ctgaccgagc gcgagcgcgc cggccggctc cgtcggctgc aggtcgttcg
     7861 ccgtcttcag gggcgcgatc gcggcctcga taccgccccg gtcgcgggcc gccgccagga
     7921 ggatctggcc gagcacccgg tgcgccgcgg cgctgcccgg gacgagctcc accgcgcgac
     7981 gcgcctgctc ctcggcctcc gggaggatcc cgaagtcgcg aagcgcctcc gcgtactcgc
     8041 ggcgaaggtg gccgtccgca gggtcgagct ccaccgccct gcggaactcc ttgagggcct
     8101 cctggaactc gccctccgcg gcgaagagcc gggccctcag gaatctctcc acggcgtctc
     8161 gtcgggccga ctcggccggg ctgagcgcct ccgaacggac cggcggagcc gccgcgacgg
     8221 gcggggcgag gatcgcgagg gcgaggacgg cggtgacggg acgaaatctc atgccgccgc
     8281 ggagcgtagc aagcgggagg ccaggaaggg agagggtccc ccccctcacg atccggcgat
     8341 ctcagaagac ggcaggcctc ccggggggct tggc
//
LOCUS       DAOONL010000137         8329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10051_length_8329_cov_16.626904, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000137 DAOONL010000000
VERSION     DAOONL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_8329_cov_16.626904"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLP50_14595"
     CDS             <1..465
                     /locus_tag="PLP50_14595"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPA52821.1"
                     /translation="AARAGVSRGALDLYAGAGFLTRALLEAGFETEAVESDRSSSTDL
                     AANLAAWKSEGLPGRGRAVARLVEEHLHRERERRDVVFADPPREGLGPGVRKALLRIA
                     PRSLFLVSCDPATFARDLAALLPRFRVAALTLLDLFPGTHHVETLARLERRG"
     gene            462..2858
                     /locus_tag="PLP50_14600"
     CDS             462..2858
                     /locus_tag="PLP50_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPA52822.1"
                     /translation="MRRSWSLRAPSPAVPAALAFAAGILLAVELPIAPSPSVASALAI
                     AAAVAVAAGLRGSRSLAGAGGLLLVVLLAFARARPALVVDREAARRTAASASEDDVFE
                     VRGRLGTYWSRSGSIWSARLDVEDARREGVPVALPGRLVLSVAGETHPGAHAGYGDLV
                     RVRGPLRLPDAGAIPQSPFGLAPEPRLPQKSAVQIAVLDGPRGAIGVPARLHRAASRR
                     LEEGLEGASPGEREAAALAGALVLGETDRLSPATVSAFRDGGVAHVLAISGLHVGLLA
                     LGLTAALGPFRPRPLTRDAIVLLATVAFAAFTGGRAPVLRAALMIGLYLGARLLGRPT
                     SPGQVFGLSALVLLAHDPSRLFDVGFLLTYAAVFGISALSPVFARPLRERLPVPLADG
                     LAATLGAELCVFPIQAAVFHVVPFVALVSNLLVVPVASVFLFAAVLHLPLLLAGPVVA
                     SLALAPLALLSDAMLGLLRLLDSVGAVRVVPTPSFAVAALLALFLAAAGVLRRRGPRR
                     AALGGAVALVFLVSARPSSAARRGEARLIALDVGQGDSFLVVTEKGRVLVDGGGTFDA
                     AYDFGRTRLLPRLAALGAVSFDAVVLSHPHPDHSRGLVALLRLVPVGRLYLPRGAPRN
                     PFLDETLAAASGGRPPVEALAAGDRFRAAGVEFDVLHPGAESYPRAPENNGSLVVGVR
                     LGSRRVLLTGDVEERAEADLVARGLDLAADVLKVPHHGSRTSTSAELLGAVRPRIALV
                     SAGRRNRFGHPGREVIERLESSGTRVFRTDRHGDVELLFRDGRIFPSRPFAGLPGGIP
                     "
     gene            2855..3784
                     /gene="miaA"
                     /locus_tag="PLP50_14605"
     CDS             2855..3784
                     /gene="miaA"
                     /locus_tag="PLP50_14605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPA52823.1"
                     /translation="MTVPRGVLAIVGATATGKSALAVEAALRLGGEVISADAFTAYRG
                     IDAGTAKPTLEERRGVPHHLIDLKEPTEAFSAGEFARLARAAAREILSRGKIPILCGG
                     TGFYVRAFFEGLFVGPPRDERLREALRLVEARRGAPWLVRALTLLDPQSGARISQRDG
                     ARAIRYLEIAVSTGRRPSELFQERPGERWDGPSVKLLLTLPRPVLYGRIERRFRDSIM
                     APLAGEVRRLLEAGVPVSAPGMAAIGYRETAALLEGRLSASEWGDTVIRQTRRYAKRQ
                     ETWFRSEPGLHPIRADAPDLVEEVVSASRPLFS"
     gene            3806..4051
                     /locus_tag="PLP50_14610"
     CDS             3806..4051
                     /locus_tag="PLP50_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPA52824.1"
                     /translation="MINKPPINVQDGFFYQLRKENTLVEVVLLSERTRLGRIRRFDKY
                     AVVLEVDGREEMIYKHAIASVATAAVRPAAPALAPAP"
     gene            4066..5403
                     /locus_tag="PLP50_14615"
     CDS             4066..5403
                     /locus_tag="PLP50_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52825.1"
                     /translation="MSPIGRAPSRTPRRERPLAGLVAAILLAVATGCASAPPPAPAAS
                     AEAAFAFRDPREGAPPPAPTARESKALDAALGALRRGDAAAASRALAVRKGEAAPALR
                     LARAYLDLLEGRRDEARAFLAEGIASHPNWIAAVEAAADIAADEGETPDALEGYRTLL
                     RLVPSDARSRERVATLRTSLAASKRASAEEALAAGDLDAARRHANSLLQLEPESVGAL
                     VLLSKTASAAGRAEDAWTWAREARRKAPSDPSVAAFAGDAAARAGRWADAATLYEEAA
                     ASDPASAGKAEDARFEFKVQNLPEAARAAAESPRLTRAQLALLLWWTVPEFREALVPP
                     GTEIAVDVVGRSDQGPLVKAIGLGFLDVSPETHRVGTETAVGRGELAGALRRVALLAG
                     RGRAPKGCLSAAGADALADCGILGRTPTRYVTGREALRALEKAARIGREGGTR"
     gene            5400..6077
                     /locus_tag="PLP50_14620"
     CDS             5400..6077
                     /locus_tag="PLP50_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPA52826.1"
                     /translation="MNAAPDLFAKVSLFEGLTTADRVALARAATVRTYRRGERIVTQG
                     QPGDSFFVLVKGRVAVSILSPEGREVVLSTLSAGDCFGEMALLDEAPRSATVTAAERS
                     ELAVLTREAFFELLKGNFVLFRGLLSTFSRRLRHANATIEGLASLDVKGRLARYFRDL
                     ADERGRKAGGGWTVVVRPSQREIADTIGSSRETVSRTMSQLARENLIVPKGKAIYVRL
                     EGETSGG"
     gene            6084..6701
                     /locus_tag="PLP50_14625"
     CDS             6084..6701
                     /locus_tag="PLP50_14625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPA52827.1"
                     /translation="MELRPWTLPNLLTFARLVAIPFLINAVLEGQHGQAFAIFFAMAV
                     SDFVDGYLARHFGMASPLGALLDPAADKLLLVSTFIVFALKSTPSNIHIPLWLVLLTV
                     ARDVLIVVVALVMALGLGIKSFPPTLLGKANTFAEISTVVAILMNNIDRMPAWVAEAC
                     FVATFLLTIASGVHYIFRASSAIASAAPGGQPATGESPPAPPPSP"
     gene            6744..6819
                     /locus_tag="PLP50_14630"
     tRNA            6744..6819
                     /locus_tag="PLP50_14630"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6777..6779,aa:Gly,seq:ccc)
     gene            complement(6886..7503)
                     /locus_tag="PLP50_14635"
     CDS             complement(6886..7503)
                     /locus_tag="PLP50_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52828.1"
                     /translation="MADTPPGVRGPTAADRLGEKAEPRTALESLPPFTFRHGLIPAVQ
                     SVVSERFDAGAPELAVVYAEVTALSATALLSGEKVPYSVFTGKVFVRLSGEEGAVVGP
                     GVEPASGLLVHAWAGRVRLSGRLEVADLSTRHFGTWLAGSGLPPGIRFPRAVWAFDDE
                     VPRAFRYVSDAADSVRPPSSLSPARADAVASAVREVLARFRTGSG"
     gene            7795..>8329
                     /locus_tag="PLP50_14640"
     CDS             7795..>8329
                     /locus_tag="PLP50_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA52829.1"
                     /translation="MKTSGLPADSLEMTLQSLRDFAEQRLPVSRLLQLDHADEFPVED
                     VRALCDPNTLGIQLVFIQEEYGGFGGSAWDVYRTCELMAAIDLGVATGVLATFLGSDP
                     ISFGGTEEQKKHWLGRIAEEGLLFAYGATEPAAGSDLGALKTTATPVVEDGKTVGYKL
                     NGAKQWISNGGYADVYTI"
BASE COUNT          997 a         3215 c         2868 g         1249 t
ORIGIN      
        1 gcggcccgcg ccggggtctc gcgcggtgcc ctcgacctct atgccggcgc cgggttcctg
       61 acccgggcgc tgctggaggc cgggttcgag acggaggcgg tcgagtcgga ccgctcctcg
      121 tcgaccgacc tcgccgcgaa cctcgcggcg tggaagagcg aggggctccc cggtcgcggg
      181 cgcgccgtcg cccggctcgt cgaggagcac ctccaccggg agcgggagcg gcgggacgtc
      241 gtcttcgccg acccgccgcg cgaggggctc ggtcccggcg tgaggaaggc gctcctccgg
      301 atcgcgccga ggagcctctt cctcgtctcg tgcgaccccg ccacgttcgc gcgggacctc
      361 gccgcgctcc tcccgcgctt ccgggtcgcg gccctgacgc tcctcgacct cttcccggga
      421 acgcaccacg tcgagacgct cgcgaggctc gagaggcgcg gatgaggcgc tcctggtcgc
      481 tccgggcacc gtcgccggcg gtccctgcgg ccctcgcctt cgccgcgggg atcctcctcg
      541 ccgtcgagct ccccatcgcc ccctctccgt ccgtggcctc tgctctcgcg atcgccgcgg
      601 ccgtcgccgt cgccgccggt ctgaggggat cgcggtcgct cgcgggcgcc ggcggcctcc
      661 tcctcgtcgt ccttctggcc ttcgcccgcg cccggccggc gctggtcgtc gatcgcgaag
      721 cggcccgtcg gacggccgcg tccgcgagcg aggacgacgt cttcgaggtg cgcggtcgac
      781 tcggcaccta ctggtcgcgg agcggatcga tctggtccgc gcgcctcgac gtcgaggacg
      841 cgcggcggga gggcgtgccc gtcgcgcttc cgggccgcct cgtcctgagc gtggcggggg
      901 agacccaccc gggtgcgcac gccggctacg gcgacctcgt ccgcgtgcgc ggccccctgc
      961 gcctgccgga cgcgggcgcg attccgcaga gcccgttcgg gctggctccc gagccccggc
     1021 tgccacagaa gtccgcggtg cagatcgcgg tcctcgacgg tccccggggc gcgatcggcg
     1081 tccccgcgcg cctgcaccgc gccgcgagcc ggcggctcga ggaggggctc gagggagcct
     1141 ctcctggtga acgagaggcc gcggccctcg ccggggcgct cgtcctgggc gagaccgacc
     1201 ggctctcccc ggccaccgtc tccgcctttc gggacggcgg cgtcgcccac gtcctcgcga
     1261 tctcgggcct gcacgtcggg ctcctcgcgc tcgggctcac ggcggcgctc ggtcccttcc
     1321 gtccgcggcc gctcacgcgg gacgcgatcg ttctcctcgc caccgtcgcg ttcgccgcgt
     1381 tcaccggcgg ccgcgcgccc gtcctccgcg cggcgctcat gatcggcctc tacctcggag
     1441 cccggctgct cgggcgtccg acctcgcccg gccaggtctt cgggctctcg gcgctcgtcc
     1501 tcctcgcgca cgacccctcc cgtctcttcg acgtcggctt cctcctgacg tacgccgccg
     1561 tgttcggcat ctccgcgctc tctcccgtct tcgctcggcc gctgcgggag cggcttcccg
     1621 tccctctcgc cgacggtctc gcggcgacgc tcggcgcgga gctctgcgtc ttcccgatcc
     1681 aggccgccgt cttccacgtc gtccccttcg tggcgctcgt ctcgaacctc ctcgtcgtgc
     1741 cggtcgcgag cgtcttcctc ttcgcggccg tcctccacct gccgctcctc ctggccgggc
     1801 cggtcgtcgc ctcgctcgcg ctcgccccgc tcgcgctcct ctccgacgcg atgctcggcc
     1861 tcctccggct cctcgactcg gtcggagccg tgcgcgtcgt cccgaccccc tcgtttgccg
     1921 tcgcggcgct cctcgcgctc ttcctcgccg ccgcgggtgt cctccggcgt cgcgggccgc
     1981 gtcgcgccgc cctcggcggg gccgtcgccc tcgtcttcct cgtctcggcc cgcccgtcgt
     2041 cggccgcgcg acgcggcgag gcgcggctca tcgcgctcga cgtcggacag ggggactcct
     2101 tcctcgtcgt cacggagaag gggcgcgtcc tcgtcgacgg aggcgggacg ttcgacgccg
     2161 cgtacgactt cggccggacg cgcctcctcc cgcggctcgc cgccctcggg gccgtctcgt
     2221 tcgacgcggt cgtcctgtcg cacccgcatc ccgaccactc gcgcgggctc gtggcgctgc
     2281 tccgcctcgt tccggtcggc cgcctctacc tccctcgggg cgcgccgcgc aacccgttcc
     2341 tcgacgaaac gctcgccgcg gcctcgggcg gacgcccgcc cgtcgaggcg ctggccgccg
     2401 gcgacaggtt ccgggcggcg ggagtggagt tcgacgtgct ccaccccgga gcggagagct
     2461 atccgcgcgc gccggagaac aacggctcgc tcgtcgtcgg cgtccgtctc ggctcccgac
     2521 gcgtcctgct cacgggggac gtcgaggaga gagccgaggc cgacctcgtc gcgcgcggcc
     2581 tcgacctcgc ggccgacgtc ctgaaggtcc cgcaccacgg cagccgcacc tccacgagcg
     2641 cggagctcct gggggcggtc cgcccccgga tcgctctcgt gagcgcgggc cggcggaacc
     2701 ggttcgggca tccggggcgc gaagtgatcg aacgcctcga gtcctccggg acccgcgtct
     2761 tccggacgga ccggcacggc gacgtggagc tcctcttccg cgacgggagg atcttcccgt
     2821 cgcgcccgtt cgcgggactt ccgggaggga tcccgtgacg gtcccgcggg gcgtcctcgc
     2881 gatcgtcggg gcgacggcga cggggaagtc ggccctcgcc gtcgaggcgg ccctccggct
     2941 cggtggcgag gtgatctccg ccgatgcttt cacggcctac cgggggatcg acgcggggac
     3001 cgcgaagccg accctcgagg agcgccgcgg cgttccgcac cacctgatcg acctgaagga
     3061 gccgaccgag gcgttcagcg cgggggagtt cgcccggctg gcccgtgcgg cggcccgaga
     3121 gatcctctcg agagggaaga tcccgatcct ctgcggcggg accggcttct acgtccgcgc
     3181 cttcttcgag ggcctcttcg tcgggcctcc gcgcgacgag cggctgcgcg aggcgctgcg
     3241 cctcgtcgag gcgcgccgtg gcgcgccgtg gctcgtccgg gcgctgacgc tcctcgaccc
     3301 gcagagcggg gcacggatct cgcaacggga cggagcgcgt gccattcgat acctggagat
     3361 cgccgtgtcg acaggccgcc gtccttcgga gctcttccag gagaggcccg gagagcgctg
     3421 ggacggaccg tccgtgaagc tcctactcac gttgccgcgc cccgtcctct atggaagaat
     3481 cgaaaggagg tttcgagact ccatcatggc cccgctcgcc ggcgaggtga ggcgcctcct
     3541 tgaggcgggc gtaccggtga gcgctccagg catggccgcc atcggctaca gggagacggc
     3601 ggccctcctg gagggccggt tgtcggcctc cgagtggggc gacacggtga tccgccagac
     3661 gcgccgctac gcgaagcggc aggaaacgtg gttccggtcc gagccgggtc ttcatcctat
     3721 ccgggccgac gcgcccgacc tcgtcgaaga ggtcgtctcc gcgtctcgcc cgctcttctc
     3781 ctgatctttt cgaagggaga cgtcgatgat caacaagccg ccgatcaacg tacaggacgg
     3841 tttcttctac cagctccgca aggaaaatac gctcgtcgag gtggtcctgc tctccgagcg
     3901 gacgaggctc ggccgcatcc ggcggttcga caagtacgcc gtggtcctcg aggtcgacgg
     3961 gcgcgaggag atgatctaca agcacgccat cgcgtccgtc gccacggccg ccgttcgccc
     4021 ggccgcgccg gccctggcgc cggccccctg agggctgcca ccggattgag cccgatcggt
     4081 cgagccccgt cccgaacgcc ccgccgcgaa cggccgctcg ccggcctcgt cgccgcgatt
     4141 ctcctcgccg ttgccacggg ctgcgcgtcg gcgccgccgc cggctccggc cgcctccgcg
     4201 gaggcggcct tcgcgtttcg cgacccgcgg gagggggcgc cgccccccgc gccgacggcg
     4261 cgggagtcga aggccctcga cgcagccctc ggggcgctcc gaaggggcga tgccgccgcg
     4321 gcgtcgcgcg ccctcgcggt ccgcaagggc gaggccgcgc ccgcgctgcg gctcgcacgc
     4381 gcctacctcg acctgctcga aggcaggcgg gacgaggcgc gcgccttcct ggccgagggg
     4441 atcgcgagtc acccgaactg gatcgcggcc gtcgaggcgg ccgccgacat cgcggccgac
     4501 gagggagaga ccccggacgc gctggaaggc taccggactc tgctgcgcct cgttccctcc
     4561 gacgccaggt cccgggagcg ggtcgcgacg cttcggacct cccttgccgc ctcgaagcgg
     4621 gcctcggccg aagaggccct cgcggccggg gacctcgacg cggcccgccg ccacgcgaac
     4681 tcgctgctgc agctcgagcc ggagtcggtg ggggcgctcg tccttctctc gaagacggct
     4741 tcggccgccg gccgggccga ggacgcctgg acgtgggcgc gggaggcccg ccgcaaagcc
     4801 ccctccgatc cgtccgtcgc ggccttcgcc ggcgatgcgg ccgcccgggc ggggcgctgg
     4861 gccgacgcgg cgaccctcta cgaggaggcg gccgcctcgg atccggcgtc ggcgggcaag
     4921 gcggaggacg cgcgcttcga gttcaaggtc cagaacctgc cggaggccgc gcgagcggcg
     4981 gcggagtccc cgcgcctgac gcgcgcgcag ctcgcgttgc tcctctggtg gacggtcccc
     5041 gagtttcgcg aggccctcgt ccctccggga acggagatcg cggtcgacgt cgtcggccga
     5101 tccgaccagg ggccgctcgt caaggcgatc ggcctcgggt tcctcgacgt ctcgcccgag
     5161 acccatcgtg tcggaacgga gaccgccgtt ggccgcggcg agctcgccgg cgcgctgcgg
     5221 cgagtcgcgc tcctcgccgg caggggtcgc gccccgaagg gctgcctctc ggctgcgggc
     5281 gccgacgcgc tcgccgattg cgggatactc ggcagaacgc cgacccgcta cgtgaccggc
     5341 cgcgaggccc tccgggcctt ggagaaagcc gcccgaatcg ggcgtgaggg aggtaccaga
     5401 tgaacgccgc ccccgacctc ttcgcgaagg tttccctctt cgaggggctg acgacggccg
     5461 accgggtcgc cctggcgagg gcagcgaccg tccggaccta ccgccggggc gagcggatcg
     5521 tcacccaggg acagccgggc gattccttct tcgtcctcgt caaggggcgc gtcgccgtct
     5581 cgatcctctc gccggagggg cgggaggtcg tcctctccac cctctccgcg ggcgactgct
     5641 tcggcgagat ggcgctcctc gacgaagcac cgcgctccgc caccgtcacg gccgccgagc
     5701 gctccgagct cgccgtcctg acgcgcgagg cgttcttcga gctcctgaag gggaacttcg
     5761 tcctcttccg cggcctcctc tccaccttct cccgccggct ccgccacgcg aacgcgacga
     5821 tcgagggact cgcctccctc gacgtgaagg gccggctcgc gcgctacttc cgcgacctcg
     5881 ccgacgagag ggggaggaag gcgggcggcg gctggacggt cgtcgtccga ccgtcccagc
     5941 gcgagatcgc cgacacgatc ggctcgagcc gggagaccgt ttcccgcacg atgtcccagc
     6001 tcgcccgcga gaacctgatc gtcccgaagg ggaaggccat ctacgttcgg ctcgaggggg
     6061 agacctcggg cggctgaggc gcgatggagc tccgtccctg gaccctgccg aacctgctga
     6121 ccttcgcgcg gctcgtcgcg atcccgttcc tgatcaacgc ggtcctcgag ggtcagcacg
     6181 gtcaggcctt cgcgatcttc ttcgcgatgg ccgtcagcga cttcgtcgac ggctacctgg
     6241 cccggcactt cggcatggcc tccccgctcg gcgcgctcct cgacccggcg gccgacaagc
     6301 tcctcctcgt ctcgaccttc atcgtcttcg ccctcaagtc caccccctcg aacatccaca
     6361 tccccctctg gctcgtcctc ctgacggtcg cccgggacgt cctgatcgtc gtcgtcgccc
     6421 tcgtcatggc cctcggcctc ggcatcaagt cgttcccgcc gacgctcctc gggaaggcga
     6481 acacgttcgc cgagatctcg acggtcgtcg cgatcctgat gaacaacatc gaccggatgc
     6541 cggcctgggt cgcggaggcc tgcttcgtgg cgaccttcct cctgacgatc gcctcgggcg
     6601 tccactacat cttccgagcc tcgtccgcga tcgccagtgc cgcaccgggg gggcagccgg
     6661 cgaccgggga atcccctccc gccccccctc cctcgccttg acgccgcgcc ccggaaacgg
     6721 tacaaacccc tcccacgcct cgagcgggcg tagctcagtt ggtagagcgc cagcttccca
     6781 agctggatgt cgcgagttca accctcgtcg cccgctccag ttcttgccct ccggttctcg
     6841 cgcgacacgg gttgccttga cgagtccgga tccgagagct ccgcttcagc ccgaaccggt
     6901 ccggaaccgc gcaagaacct cgcgaaccgc gctcgccacg gcgtccgcgc gagcggggct
     6961 cagcgacgac ggaggccgca ccgaatccgc cgcgtcggag acgtagcgga acgctctcgg
     7021 gacctcgtcg tcgaaggccc agaccgctcg agggaatcgg attcccggcg gcagcccgga
     7081 accggcgagc cacgtcccga agtggcgggt cgagagatcc gccacctcca gccgtcccga
     7141 gagacggacg cgtcccgccc aggcgtgaac gaggaggccg ctcgcgggtt cgacgcctgg
     7201 tccgacgacc gccccctcct ccccggagag acggacgaag accttccccg tgaagacgga
     7261 gtagggaacc ttctccccgg acaggagcgc ggtcgcggaa agagccgtca cctccgcgta
     7321 gacgaccgcg agctcgggag cgccggcgtc gaaccgctcg ctcacgacgg actggacggc
     7381 cgggatgagg ccgtgacgaa acgtgaaggg gggaaggctc tcgagcgccg tgcgaggctc
     7441 tgccttctcg ccgagcctgt cggcggctgt gggcccgcgg acgcctggag gcgtgtctgc
     7501 catctcgacc cgacctcgcc cggacgatac ggcaacacgt cggcgctgag ccttcgctca
     7561 ctccaaatcg ctgatccgat gaatgttagg gcgctgcaac gcgccatttc ctagacctgt
     7621 tctcgatttg tgtcccggcc tcgcgccggg taaagtgccc cggtttcgct gtggctccct
     7681 acctcccatc cccctagctc cgaagcgctt ccgaggatcc gcagagggtc ctcttcgttt
     7741 gtccggcggg ccccgcgggg gagcccgggc cgccggggga aagagagaac cgagatgaag
     7801 acctccggcc ttcctgccga ttcgctcgag atgaccctcc agagcctgcg cgacttcgcc
     7861 gagcagcggc tcccggtctc gcgcctcctc cagctcgacc acgccgacga gttcccggtc
     7921 gaggacgtcc gggcgctctg cgacccgaac acgctcggca tccagctcgt cttcatccag
     7981 gaggagtacg gcggcttcgg cgggagcgcc tgggacgtct accggacctg cgagctgatg
     8041 gccgcgatcg acctcggcgt ggcgaccggg gtcctcgcga cgttcctcgg ctccgacccg
     8101 atctccttcg gcgggaccga ggagcagaag aagcactggc tcggccggat cgcggaggag
     8161 ggcctcctct tcgcgtacgg cgccacggag ccggcagccg gctccgacct cggcgccctg
     8221 aagacgacgg cgacgccggt cgtcgaggac gggaagaccg tcgggtacaa gctgaacggc
     8281 gccaagcagt ggatcagcaa cggcggctac gccgacgtct acacgatcc
//
LOCUS       DAOONL010000138         8299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10098_length_8299_cov_19.440442, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000138 DAOONL010000000
VERSION     DAOONL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10098_length_8299_cov_19.440442"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLP50_14645"
     CDS             complement(<1..304)
                     /locus_tag="PLP50_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA52830.1"
                     /translation="MISLPAGSYALVTGASAGIGEAIARSLARRRVPQLLVARSAEKL
                     SALAAELRASSGVAVEAVPLDLEVDGAPERLVAATEGVGKPVGLLVNNAGFGWYGPQ"
     gene            complement(301..1095)
                     /locus_tag="PLP50_14650"
     CDS             complement(301..1095)
                     /locus_tag="PLP50_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA52831.1"
                     /translation="MSHPKRNWALVLGASSGFGEAVSIALAKAGRNVFGVHLDRRATL
                     PNVERIAGEIAGAGGKAVFFNVNAADAEKRGEVVAAMKAALAENGGALDVVLHSLAFG
                     TLKPFLGDEKERMTKAQMEMTLDVMAHSLVYWVQDLAAAELLGAGAHVFAMTSAGGHR
                     IWPSYGAVSAAKAALESHCRQLAVELVARGIAVNAIRAGVTDTPALRKIPGNVEMIER
                     VLAVHPAGRLTTPQDVANVIVAMARPETAWMTGNVLGVDGSEDLLG"
     gene            1154..1906
                     /locus_tag="PLP50_14655"
     CDS             1154..1906
                     /locus_tag="PLP50_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPA52832.1"
                     /translation="MRFDALRRLLEKLQRSGKKTLDVEELSGDFTDSELAFGRLVLPL
                     VEDLDAMKKLLEDLSRRARAMQTVLDRLPVAALVLDAEGSLLAMNRAAKDHFGGAAIT
                     SAVLRAARRALQGTEESSATVDDPRRKGKRLRLVLADVAEEKAEGPSEKPAVVFVVSN
                     DSPPPVDEADLVTRFDLTKVEASVVALVAHGLANKEVGEALGVSVETVRSHLASAFRK
                     TGARNRAGLAALAYGARFGQGPISGAFAAVEE"
     gene            1903..>2643
                     /locus_tag="PLP50_14660"
     CDS             1903..>2643
                     /locus_tag="PLP50_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPA52833.1"
                     /translation="MIPAALAARLAAGPLVLDAALGTELTRRGSPSAPPLWSARAVLE
                     RPDLVLALHRENVGAGADVLTAATFRTQPALLGAAAREATRCAVGLAREAAAERPGVL
                     VAGSIAPVADCWRPDLVPADAALARLHALHAGALAAAGADLLLLETFGTAREWLAAAR
                     AAAATGLPVVACATTGAGGALLSGEPLGEAARSLLALPSPPVALGVNCVPARRVAASL
                     ALLATAAPFVPLAAYGNTGLPLDEGAGLF"
     assembly_gap    2644..2743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2744..4425)
                     /locus_tag="PLP50_14665"
     CDS             complement(<2744..4425)
                     /locus_tag="PLP50_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPA52834.1"
                     /translation="MRRVVSALSVLLLLAPAAGFAQPVQQTSGKVLGVSVPLEVSEQF
                     LGTSAGRTVVRFTLSFSRTDLREKAGLAPRVYPFFVVGEARSASGEVVDAFREPVDVD
                     LSDAELGKALRASFLRALPPGSTTLGLRLEAENGRSMGLRSVTFDVPKMTSEFRADDA
                     GPLTAPAILLEEKNREDVPSGDLIRFLPPQREVPVGLLRIECEVKPPISRVEFHLGDK
                     LILARNRPPFTVEIDLGTIPRKQTLRALGFDRLGNFVDADAWALNEKEARLAVRVLDL
                     PKAKGKADSDVKVAVQSIAGAVAKRVALFLDEKKVAEWTAPPYRASVAAAEVEKATLM
                     RASAWDAEGKEYSDFRMLKGDERLLAKVDVNLVELNVSVFDEAGRFAKGLTKDDFTVL
                     EDGAVQDLSAFEFAESLPMALGLVLDGSGSMDKSMPLVKQAATEFVSNLVGEKDQGFV
                     IEFRERPTLLAPMTSNRASLIRAIGETRAGGATAFHDSVILGLYQFRSLAGKKALVVL
                     TDGKDNHSSTDWPTLKRYARTAGVPIFVVGLDLGLFDVGLKSRLKELAEDTGG"
     gene            complement(4448..5512)
                     /gene="dusB"
                     /locus_tag="PLP50_14670"
     CDS             complement(4448..5512)
                     /gene="dusB"
                     /locus_tag="PLP50_14670"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPA52835.1"
                     /translation="MPLVYQTPGTRPCVVDPPLVLAPMAGITDGTYRLLLRRMGGVGL
                     VTMEFISSEGLTRGARRTERLMRWEPEERPLSIQIYGSDAARMAEAARRVEEEGVDVC
                     DINMGCPANKILKGCAGAGLMRDLTRARGIIEACRKVLPTTPLTVKFRAGITEETTNF
                     VELGKICEGEGVAAVALHPRTAKQFYSGRADWSRIARLKEAVRIPVVGNGDVETPEDV
                     LRMLRETGCDAVMIGRATMKNPWVFRQAADLLAGRPYRVATLEERRDVILQHFRLIRE
                     AEEDVPKRMMGKLKTFTGWYTHGVPKGTELRRRIQSLPTPEAFLEAVESFFALRIEER
                     DGRVSAPEGEGAPPVRPVLA"
     gene            complement(5557..6420)
                     /locus_tag="PLP50_14675"
     CDS             complement(5557..6420)
                     /locus_tag="PLP50_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52836.1"
                     /translation="MKPYAAILASALIATLPLAAQTPVDPKSPIPGFPDGRGPKGPVS
                     IKLLSPAADEVIPMPAAEPGKPAPKGARVEVKVSVENYEIFKDEATGKGQHVQLVLDG
                     RALPNWYQVDKAWIFPSLPKGTHTLLAFLQRPWNETLREQGAFAAVTFHVGEKNGKPA
                     FDPAQPTITVAAPRGKFEGDQATKVLFDVFVTGCTVGPATDPEACQIIYRLNQDPQQV
                     LTEWKPVFWENVPPGKHSFIAALYKGEKRIEAPFALTQGFFEVAAGAPAAAAPAAAPA
                     AAPAAPATPAG"
     gene            complement(6512..7204)
                     /locus_tag="PLP50_14680"
     CDS             complement(6512..7204)
                     /locus_tag="PLP50_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52837.1"
                     /translation="MSTDVPNGARPVAGRTPLAALSLLAALLVLLPACGKMLSPPPQP
                     RTDGYSGLVTVRAAGAELAKFRIDVRGEALRRTMSDAKDARYFVRESAAGPVWEVDPA
                     SKSYREATEEALFAHLDDFPLGADFNHAAEANRRGITKYHRESDAVFAGNACAIWRYA
                     DDPEVLNSPTTTYWMAPALGGVVIRKVRSVPKADGSDETTYFELTHLRAGVDPERFRV
                     PEGFRKDGAGPG"
     gene            complement(7201..>8299)
                     /locus_tag="PLP50_14685"
     CDS             complement(7201..>8299)
                     /locus_tag="PLP50_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52838.1"
                     /translation="GGDRPSRPERPRREAGPGERPVTPRPRPDRRPAARPAPRAEVVA
                     DAMPAEVRREMKPRPPRELPAGAYPLPLSTLDWLLSRENPAARYVAFRDLLHRPPKDI
                     ERRKARQGLLRDPFLRDALPHLRGMLPVGAPADFAAPLDAGTSFVLLLLGIGCDADVP
                     ELQHAADLLLARWERVFVEIERGETPAVGSGFVAACRALLRLGYAEDDRLLGAAELLS
                     RRRVASESVSGGVAGDLLVLSGIPEARRSALLQNAIAFSVERATAVELPALSVDAQKT
                     GIGVGDPSDLLEILGALVAAGAPRTPGLDEALSRLAARADHRGRFKLERPVGVRLPVP
                     LEREGELSRWVTLRALVVMQHFLGLTITGAP"
BASE COUNT         1108 a         3062 c         2922 g         1107 t
ORIGIN      
        1 cctgcggccc gtaccagccg aagcccgcgt tgttcacgag gaggccgacc ggcttcccga
       61 ccccctccgt cgcggcgacg agccgctccg gggcgccgtc gacctcgagg tcgagcggga
      121 ccgcctccac ggcgacgccg gagctcgcac gcaactccgc cgcgagcgcg gagagcttct
      181 ccgcggagcg ggcgacgagc agctgcggga ccctcctccg cgcgagggag cgggcgatcg
      241 cctccccgat cccggccgag gcgccggtga cgagcgcgta cgacccggcc gggagggaga
      301 tcaaccgagg aggtcctcgg agccgtccac gccgaggacg ttccccgtca tccacgccgt
      361 ctcgggccgc gccatcgcga cgatgacgtt cgcgacgtcc tgcggggtcg tcagccgccc
      421 ggccgggtgg accgcgagga cccgctcgat catctcgacg ttcccgggga tcttccggag
      481 ggccggcgtg tcggtcacgc cggcgcggat cgcgttgacg gcgatgccgc gcgcgacgag
      541 ctcgaccgcg agctggcggc agtggctctc gagcgccgcc ttcgcggcgg agaccgcgcc
      601 gtaggagggc cagatccggt gcccgcccgc cgaggtcatc gcgaagacgt gcgcgccggc
      661 gccgaggagc tccgccgcgg cgaggtcctg cacccagtag acgagggagt gggccatgac
      721 gtcgagcgtc atctccatct gcgccttcgt catccgctcc ttctcgtcgc cgaggaaggg
      781 cttgagcgtc ccgaaggcga gcgagtggag gacgacgtcg agcgccccgc cgttctccgc
      841 gagggccgcc ttcatcgcgg cgacgacctc cccccgcttc tccgcgtcgg cggcgttcac
      901 gttgaagaag acggccttcc ccccggcgcc cgcgatctcg cccgcgatcc gctctacgtt
      961 cggcagggtc gcgcgacggt cgaggtggac gccgaagacg ttccgtccgg ccttcgcgag
     1021 ggcgatcgag accgcctctc cgaaccccga ggacgcgccg aggacgaggg cccagttccg
     1081 cttcggatgc gacatggcgc ggattatcgg cacccccttc ccccctgtat agtgccgctc
     1141 gaggcccgat ccgatgcgat tcgacgccct cagacgcctc ctcgagaagc tccagcgaag
     1201 cggcaagaag acgctcgacg tcgaggagct ctcgggcgac ttcaccgaca gcgagctcgc
     1261 gttcggacgc ctcgtcctcc ccctcgtcga ggacctggac gcgatgaaga agctcctcga
     1321 ggacctctcg cgccgcgctc gcgcgatgca gaccgtcctc gatcgcctcc ccgtcgccgc
     1381 gctcgtcctc gacgccgagg ggagcctcct cgcgatgaac cgggcggcga aagaccactt
     1441 cggcggcgcc gcgatcacgt cagccgtcct tcgcgcggca cgtcgcgccc tacaggggac
     1501 cgaggagtcc tccgcgacgg tcgacgatcc ccgccggaag gggaagaggc tccgcctcgt
     1561 cttggccgac gtcgcggaag agaaggccga ggggccgtcc gagaagcccg ccgtcgtctt
     1621 cgtcgtctcg aacgactcgc ctcccccggt cgacgaagcc gacctcgtca cacggttcga
     1681 cctgacgaag gtcgaggcct ccgtcgtcgc gctcgtcgcg cacggcctcg cgaacaagga
     1741 ggtcggggag gcgctggggg tctcggtcga gacggtgcgt tcccacctgg cgagcgcatt
     1801 ccggaagacg ggcgcgcgca accgcgccgg tctcgccgcc ctcgcctacg gggcccgctt
     1861 cggccagggg ccgatctccg gcgccttcgc cgccgtcgag gagtgatccc ggccgcgctc
     1921 gccgcccggc tcgccgcagg gccgctcgtc ctcgacgccg ccctcggcac cgagctcacg
     1981 cggcgcggct ccccctccgc gccgcccctc tggagcgccc gcgcggtcct cgagcgaccc
     2041 gacctcgtcc tcgccctcca tcgcgagaac gtcggcgcgg gagcagacgt cctgacggcg
     2101 gcgacgttcc ggacgcagcc ggccctcctc ggcgccgccg cccgcgaggc gacccggtgc
     2161 gcggtcggcc tcgcccgcga ggcggccgcc gagcgacccg gcgtcctcgt cgccggctcg
     2221 atcgcgcccg tcgccgactg ttggcggccc gacctcgtcc ccgccgacgc cgcgctcgcg
     2281 cgcctccacg cgcttcacgc cggggcactt gcagcggccg gcgccgacct cctcctcctc
     2341 gagacgttcg gcacggcccg cgagtggctc gcggccgcgc gggccgccgc ggcgaccggc
     2401 cttcccgtcg tcgcctgcgc gacgaccggc gcgggcggcg cgctcctctc gggcgagccg
     2461 ctcggggagg cggcccgctc gctcctcgcg ctcccgtcgc ctcccgtcgc gctcggcgtg
     2521 aactgcgtcc cggcccggcg ggtcgccgcg agcctcgccc tcctcgccac cgccgcgccg
     2581 ttcgtcccgc tcgccgcgta cgggaacaca ggtctcccgc tcgacgaagg agccggcctc
     2641 ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccgg tgtcctccgc
     2761 gagctccttc aggcgcgact tcagccccac gtcgaagagg ccgaggtcga gcccgacgac
     2821 gaagatcggc acccccgcgg tccgcgcgta gcgcttcagc gtcggccagt cggtcgacga
     2881 gtggttgtcc ttgccgtccg tcaggacgac gagcgccttc ttgccggcga gcgaacggaa
     2941 ctggtagagg cccaggatga ccgagtcgtg gaacgccgtc gcgccgccgg cgcgcgtctc
     3001 gccgatcgcc cggatgagcg aggcgcggtt cgacgtcatc ggcgcgagga gcgtcggccg
     3061 ctcgcggaac tcgatgacga acccctggtc cttctcgccg acgaggttcg agacgaactc
     3121 cgtcgccgcc tgcttgacga ggggcatcga cttgtccatc gagcccgacc cgtcgaggac
     3181 gaggccgagc gccatcggaa gcgactcggc gaactcgaag gccgagaggt cctggacggc
     3241 gccgtcctcg aggacggtga agtcgtcctt cgtcagcccc ttcgcgaacc gccccgcctc
     3301 gtcgaagacc gagacgttca gctccacgag gttcacgtcc accttcgcga ggagccgctc
     3361 gtcccccttc agcatccgga agtcggagta ctccttcccc tccgcgtccc acgccgaggc
     3421 gcgcatcagc gtcgccttct cgacctccgc ggccgcgacg ctcgcccggt aggggggagc
     3481 ggtccactcg gccaccttct tctcgtcgag gaagagcgcg acccgcttcg cgaccgcgcc
     3541 ggcgatcgac tggaccgcga ccttcacgtc ggaatcggcc ttccccttcg ccttcggcag
     3601 gtcgaggaca cggaccgcga ggcgcgcttc cttctcgttc agcgcccagg cgtcggcgtc
     3661 gacgaagttc ccgagccggt cgaagccgag ggcgcggagc gtctgcttgc gcgggatcgt
     3721 cccgaggtcg atctcgacgg tgaacggcgg gcggttccgg gccaggatca gcttgtcgcc
     3781 gaggtggaac tcgacccggg agatcggggg cttcacctcg cactcgatcc ggaggaggcc
     3841 gaccggcacc tcgcgctgcg gcgggaggaa gcggatcagg tcgcccgagg gcacatcctc
     3901 gcggttcttc tcctcgagga ggatcgccgg agcggtgagc gggccggcgt cgtccgcccg
     3961 gaactccgac gtcatcttcg gcacgtcgaa cgtcaccgac cgcagcccca tcgaccggcc
     4021 gttctccgcc tcgagcctca gtcccagcgt ggtcgagccg ggcggcagcg cgcggaggaa
     4081 cgaggcgcgg agggccttcc cgagctcggc gtccgagagg tcgacgtcga ccggctcccg
     4141 gaacgcgtcg acgacctcgc ccgaggcgct ccgcgcctcc ccgacgacga agaacgggta
     4201 gacccgcggg gcgaggcccg ccttctcgcg caggtcggtg cgggagaagg agagggtgaa
     4261 gcggacgacc gtccggccgg cgctcgtccc gaggaactgc tcggagacct cgagcgggac
     4321 ggagacgccg aggaccttcc ccgacgtctg ctggaccggc tgggcgaagc cggcggcggg
     4381 cgccagcagg aggaggacgg agagcgcaga gacgacacgt cgcacggtga ggacctccct
     4441 cggagtctta cgcaagaacc ggacgaacgg gtggggctcc ttcgccctcc ggggcggaca
     4501 cccgtccgtc gcgctcctcg atccggaggg cgaagaacga ctccaccgcc tcgaggaacg
     4561 cttcgggcgt cgggagcgac tggatccggc ggcgcagctc cgtccccttc gggacgccgt
     4621 gcgtgtacca gccggtgaac gtcttcagct tccccatcat ccgcttcggg acgtcttctt
     4681 ccgcctcgcg gatcagtcgg aagtgctgga ggatgacgtc ccgccgctcc tcgagcgtcg
     4741 cgacccggta cggtcgcccc gcgaggaggt cggccgcctg ccggaagacc caggggttct
     4801 tcatcgtggc ccggccgatc atcacggcgt cgcagccggt ctcgcggagc atccggagga
     4861 cgtcctccgg cgtctccacg tccccgttcc cgacgacggg gatgcgcacg gcctccttca
     4921 ggcgcgcgat ccggctccag tcggctcgcc ccgagtagaa ctgcttcgcc gtccgcgggt
     4981 ggagggccac ggcggcgacg ccttccccct cgcagatctt cccgagctcg acgaagttcg
     5041 tcgtctcctc cgtgatcccg gcccggaact tcacggtcag cggcgtcgtc gggagcacct
     5101 tgcggcacgc ctcgatgatc ccgcgcgccc gcgtcaggtc ccgcatgagg cccgcgccgg
     5161 cgcacccctt caggatcttg ttcgccgggc accccatgtt gatgtcgcag acgtcgaccc
     5221 cctcctcctc cacccggcgc gcggcctcgg ccatccgggc cgcgtcggag ccgtagatct
     5281 ggatcgagag gggacgctcc tcgggctccc aacgcatcag ccgctccgtc cggcgagccc
     5341 cgcgcgtcag cccctcggac gagatgaact ccatcgtcac gaggccgacg ccccccatcc
     5401 ggcgcaggag cagccggtag gtcccgtcgg tgatccccgc catcggcgcc aggacgagcg
     5461 gcgggtcgac aacgcagggg cgtgtcccgg gggtctggta gacgagaggc atcgggagcg
     5521 ggcgcggggc agggagcccc gcgcccggga ccgggatcag ccggcgggcg tcgccggagc
     5581 cgccggagcg gcggccggag ccgccgcagg ggccgccgca gcgggggcgc ccgcggcgac
     5641 ctcgaagaag ccctgcgtca gcgcgaacgg ggcctcgatc cgcttctcac ccttgtagag
     5701 ggcggcgatg aagctgtgct tgccgggggg aacgttctcc cagaagaccg gcttccactc
     5761 ggtcagcacc tgctgcgggt cctggttcag ccggtagatg atctggcacg cctccgggtc
     5821 ggtggccggc ccgacggtgc agccggtgac gaagacgtcg aagaggacct tcgtcgcctg
     5881 gtctccctcg aacttcccgc gcggcgcggc gacggtgatc gtcggctgcg ccgggtcgaa
     5941 ggccggcttg ccgttcttct cgccgacgtg gaacgtcacg gccgcgaacg cgccctgctc
     6001 ccggagcgtc tcgttccagg ggcgctggag gaacgcgagg agcgtgtgcg tccccttcgg
     6061 cagggagggg aagatccacg ccttgtcgac ctggtaccag ttcgggagcg cccggccgtc
     6121 gaggacgagc tggacatgct gccccttccc ggtcgcctcg tccttgaaga tctcgtagtt
     6181 ctcgaccgag accttcacct cgacccgggc ccccttcggc gcgggctttc ccggctcggc
     6241 cgcgggcatc gggatcacct cgtcggcggc gggggaaagc agcttgatcg acacgggccc
     6301 cttcggcccg cggccgtccg ggaaccccgg aatcggcgac ttcgggtcga ccggtgtctg
     6361 cgccgcgagc ggcagggttg cgatgagggc ggacgcgagg atcgcggcat acggcttcat
     6421 cgaaacgact ccttggaggg cgacctcttt tccgtggcgg cccggaagcc ccgaatttag
     6481 cagacgagcc cctgcggccg tccgcccccc gtcagcccgg ccccgcgccg tccttccgga
     6541 acccctcggg gacccggaag cgctccggat cgaccccggc ccgcaggtgc gtcagctcga
     6601 agtaggtcgt ctcgtcggac ccatcggcct tcggaacgga gcggaccttc cgaatgacga
     6661 cgccgccgag ggcgggggcc atccagtagg tcgtcgtcgg agagttcagc acctcggggt
     6721 cgtcggcgta gcgccagatg gcgcaggcgt ttcccgcgaa gaccgcgtcc gactcccggt
     6781 ggtatttcgt gattccacgc cgattcgcct cggccgcgtg gttgaagtcg gcgccgagcg
     6841 ggaagtcgtc gaggtgggcg aagagcgcct cttccgtcgc ctcgcggtag ctcttcgaag
     6901 ccgggtcgac ctcccagacc ggccccgccg ccgactcccg gacgaagtac cgcgcgtcct
     6961 tcgcgtcgga catcgttcgc cggagcgcct cgccgcgaac gtcgatgcgg aacttcgcga
     7021 gctccgcccc ggccgcccgg accgtcacga ggcccgagta accgtccgtc ctgggctgcg
     7081 gcggcgggct cagcatcttc ccgcaggcgg ggagaaggac gaggagcgcg gcgaggaggg
     7141 agagcgccgc gagaggcgtc ctccccgcca ccgggcgggc gccgttcggt acgtccgtgc
     7201 tcatggggcc cccgtgatcg tcaggccgag gaagtgctgc atgacgacga gcgcgcggag
     7261 cgtcacccac cgggagagct ccccctcgcg ctcgaggggg accgggagac ggacgccgac
     7321 cggacgctcc agcttgaagc gcccgcggtg gtccgcgcgc gcggccaggc gggagagcgc
     7381 ctcgtcgagg cccggcgtgc gcggcgcacc ggccgcgacg agggccccga ggatctccag
     7441 caggtcggac gggtctccga cgccgatccc ggtcttctgg gcgtccacgg agagcgcggg
     7501 gagctccacg gccgtggccc gctcgacaga gaacgcgatc gcgttctgca ggagcgccga
     7561 gcgtctcgcc tccgggatcc ccgagaggac gaggaggtcc cccgcgacgc caccggagac
     7621 gctctcggaa gcgacccgtc gccgcgagag gagctcggcc gctccgagga gccggtcgtc
     7681 ctccgcgtag ccgagacgaa ggagggcgcg gcaggcggcg acgaagccgc tcccgaccgc
     7741 cggcgtctcg ccccgctcga tctcgacgaa gacccgctcc cagcgggcga ggaggaggtc
     7801 ggcggcgtgc tgcagctccg gcacgtccgc gtcgcagccg atcccgagaa ggagcaggac
     7861 gaacgacgtc ccggcgtcga gaggggcggc gaagtcggcc ggggcgccga cggggagcat
     7921 cccgcgcagg tgcgggagcg cgtcgcgcag gaacgggtcg cgcagcaggc cctggcgcgc
     7981 cttgcgccgc tcgatgtcct tcggaggccg atggaggagg tcgcggaagg cgacgtagcg
     8041 ggcggccggg ttctcgcggg aaagaagcca gtcgagcgtg gagagcggga gcgggtaggc
     8101 ccccgccggc agctcgcgcg gcggccgcgg cttcatctcg cggcgcacct cggcgggcat
     8161 cgcgtcggcg acgacctccg cgcgtggcgc gggacgggcc gcggggcgtc gatccgggcg
     8221 cggccgcgga gtcacgggcc tctcccccgg tcccgcctcc cgccgcggcc gctcgggccg
     8281 cgacgggcgg tcgccgccg
//
LOCUS       DAOONL010000139         7994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10614_length_7994_cov_22.907293, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000139 DAOONL010000000
VERSION     DAOONL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_7994_cov_22.907293"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..167
                     /locus_tag="PLP50_14690"
     CDS             9..167
                     /locus_tag="PLP50_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPA52839.1"
                     /translation="MVSIDVAGDAAVAKVELDYPKVFFVDYLSLLKIDGVWKIAQKTY
                     TGTSKPAP"
     gene            264..1367
                     /locus_tag="PLP50_14695"
     CDS             264..1367
                     /locus_tag="PLP50_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52840.1"
                     /translation="MKALLPGLGTAALGLAVLFTLLLALTTKAFTLRPPGGPDAMGLV
                     VVFFLPVAAWICVLGGALSAAGRGAFDWVSRSPGLPTLAVLATVVGLGGLSVAAAMFS
                     LEVRYASRTVVGLAGGLLLPLAVVGLLGFLLWSQPAAVAAARWLRPAATGLGGLAGAA
                     FVGGLVLLATQSAEKARVAGESRQADADWETRNRKEMEALHAKQADELAALPEDAPIE
                     VFLTHLFIDKSEEHHQMALERIRALPGLTARLAARLEHPEPLEREHVLNFIAMSGTPE
                     PDWEPLVRTALVRLAADYRAEAKDLSIERIHHVKGLAWGALLAAERFGPRHFEAEARE
                     LRAAVALWPNEGPRNDALAVIDLYLAGELLPTE"
     gene            complement(1423..2379)
                     /locus_tag="PLP50_14700"
     CDS             complement(1423..2379)
                     /locus_tag="PLP50_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52841.1"
                     /translation="MNAKTIGVLFGMEDTFPWALCHEINELARRRSLAVKGEPVQVGH
                     VSQEQAFAYDVILDRISHEVPFYRTFLKCAAARGVQVVNNPFWWSADDKFFDNVVARA
                     VGVAVPRTVLLPHKEHPPNTTDASFRNMTLVDWDEVFRYLGFPIFLKPAYGGGWKDVY
                     KVHSREELFSAYDQTRDLTMMAQEAIEFEDYYRCWVAGRRKVRIMPYAPKEPHEARYS
                     AVAGKVIPDEMALRVTKDALALCDALGYDMNTVEFAVRDGIPYAIDFMNCAPDADLHS
                     VGEESFRWIVTEMAETLVERALHPIPWEPTGSWPKALGLLPR"
     gene            2472..3179
                     /locus_tag="PLP50_14705"
     CDS             2472..3179
                     /locus_tag="PLP50_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPA52842.1"
                     /translation="MRREYHAWVSHHLGRTMEFLWLGERGYPVLLFPTSMGRFWQNED
                     MGITGALADKVDAGFIQLVCVDAVDTESWYNRNAPPAYRVRRHDDYDRYLRHELVPYV
                     QHRADRPDLGVYGASFGAYHAASFAGRYPGLVTKAVLLSGLYHVGRFLDGYWDDLCYY
                     HCPEAFVANADEKTVRALRQVEWIVATGEDDTLVGENRHFAGLLEGKGIGVHAEFWPG
                     VFGHDWPFWREAVRRFL"
     gene            3322..>3628
                     /locus_tag="PLP50_14710"
     CDS             3322..>3628
                     /locus_tag="PLP50_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPA52843.1"
                     /translation="MTIRRQILVATAATALLLGGPAAAQEVKGEEHVLPNGMKLLLVH
                     RPDEPSVSAGWVAKVGSANERVGITGISHLFEHMMFKGTKTLGSKDPKRDLEIIEEQE
                     "
     assembly_gap    3629..3728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3729..4943
                     /locus_tag="PLP50_14715"
     CDS             <3729..4943
                     /locus_tag="PLP50_14715"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA52844.1"
                     /translation="PKNKLELWFWMESDRLKDPVFREFYAERDVVYEERRLRVESTPT
                     GELDEAFDSVFWHSTPYTWPVIGYSSDVANITKQQADDYFATYYAPNNLTAVLVGDFD
                     KAEALKLATEYFGRIPRGKTAPPEVITLPTKWKYDLRFEGEADTNPTAEVRWRTVPFQ
                     HKDSYALSILAELLNGRTGRLYKSMVLPEGAVATQAYAVHNGFVGPAKYAGSFAIGAE
                     AKEGKLPAEVAAKALAEIERLKTEPVPADELRKVKNNVAANSFRRLSSNFPILLQLMV
                     NEAYGDWTEINSGPKKLEAVTAADVQRVAQTYLVKENQATALVSRKAGSAPSADDAAL
                     ADVPEQVRPMIKQALQQIGSEKDAAKLKASLGQMETQAAQVPPQMKKGFDLLVTKARE
                     RLSELEKAADAK"
     gene            4963..6474
                     /locus_tag="PLP50_14720"
     CDS             4963..6474
                     /locus_tag="PLP50_14720"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA52845.1"
                     /translation="MKTLARTSALALLGALLLAPAAQGVPPHPDKLTYPKFTYQPPSA
                     KDYRVVLKSGPVAYVAEDRELPLVSINVALRGGTYLSPAGKEGLAELAGYLLARGGTA
                     TKTAEELEERLAFLAASLNSGYGADRGTVSLNLLAKDLDEGLAILREVLTAPRFQDDK
                     LKLRRDQLLNDMKSRNDDTADIEQRERGFLAFGEGYYTNRYTTKASLESITREDLLAF
                     HRKWVDPKNVTVAVAGDFGKGVSRAQMIARLDALFAKWPYKGEAAPAVPTPTHAMVPG
                     LYVVDKDVNQGRVSVMLPALSRTDPDYDAALVMNDVLGGGGFTSRITNRVRSDEGLAY
                     SAGSGIVGGVWYPATFRASFQSKARTCAYATQIVLEEMTKVREAEVTDEELQTAKSSF
                     IDTLSRNFATKAQTVNVFLDEEFTGRFAKDPEYYRGYAARIEKVTKADVQRVARRLLA
                     TDKVTVLAVGKKDELLNPDPKHPVKFPDLTGGTLTDLPLRDPFTMKPLAPAAK"
     gene            6565..>7994
                     /locus_tag="PLP50_14725"
     CDS             6565..>7994
                     /locus_tag="PLP50_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPA52846.1"
                     /translation="MRATLPVLPVALLALAATSPGPALGTPPAGKAALTLERVHAAEP
                     LVEPAPTGLRWRDSSRFTYLKRTGSGPGAQASLWQEDASTGKTEKLLDAIPAEGTGKD
                     GKPRSLPLSDAAWNEAGTALVVAAENDLWLYACESAAARRLTSDAEAEELPVFSPDGA
                     KVAYVKGNDLWFVEVGTGRVTRLTNDGSPTVLNGRLDWVYEEELAGRNGGRAYEWAPD
                     SSAIAFLRLDVSKVPEYPLVDFLPTNGKHLPQRYPKPGDPNAAPSVRIATWDPSGLAT
                     TVRSVSYDGDRELLGPELAWTPDSSAVAFTRMDRTQTTIEAFLLDRSGSAGPRSLLRE
                     TSASWINAHEPPLFLPDGSGFLWLSERSGFLHLYRYGMDGSLAGQVTRGEWAITDTPR
                     LDARAGTVTFTATEADPRERHHYRVRLDGTGLTRLTSRKGTHAAIPSPDGRWVLDSWS
                     AVDTPTRVDLVRGDGTLLRPVFVPKTS"
BASE COUNT         1200 a         2985 c         2607 g         1102 t
ORIGIN      
        1 ggcgccggat cgtctcgatc gacgtcgccg gcgacgccgc cgtcgcgaag gtggagctcg
       61 actacccgaa ggtcttcttc gtcgactacc tgtcgctcct gaagatcgac ggcgtgtgga
      121 aaatcgccca gaagacctac accgggacct cgaagccggc cccctgacgt tccgctcgac
      181 gtgcggctcc cggcatccgg ggccgcgcgc ggcgtccggg cgggccgccg gccctcttcg
      241 ggtagcatcc gcgcccgaac agcatgaaag cgctcctccc gggcctcggc accgccgccc
      301 tcggcctcgc cgtccttttc acgctcctcc tcgccctgac gacgaaggcg ttcacgctcc
      361 ggccgccggg cggtcccgac gcgatggggc tcgtcgtcgt cttcttcctc cccgtcgccg
      421 cctggatctg cgtcctcggc ggtgcgctct ccgcggccgg gcgcggcgcc ttcgactggg
      481 tgagccgatc gccgggcctg ccgacgctcg ccgtcctcgc gaccgtcgtc ggcctgggcg
      541 ggctctcggt cgcggcggcg atgttctccc tggaggtccg ctacgcctcg cgcaccgtgg
      601 tcggcctcgc cggcgggctc ctcctgcccc tcgcggtggt cggcctcctc ggcttcctgc
      661 tctggtcgca gcccgcggcg gtcgcggccg cgcgctggct ccgtcccgcg gcgaccggcc
      721 tcggcggcct cgccggggcc gcgttcgtcg gcggcctcgt cctcctcgcg acgcagtccg
      781 cggagaaggc ccgcgtcgcc ggggagtcgc gtcaggcgga cgccgactgg gagacgcgga
      841 accggaagga gatggaggcg ctccacgcga agcaggcgga cgagctggcc gccctgcccg
      901 aggacgcgcc gatcgaggtg ttcctcacgc acctcttcat cgacaagagc gaagagcacc
      961 accagatggc cctcgagcgg atccgggcgc tcccgggcct cacggcgcgg ctcgccgcgc
     1021 gcctcgagca ccccgagccg ctcgagcgcg agcacgtcct gaacttcatc gcgatgtcag
     1081 ggacgcccga gccggactgg gagcctctcg tgcggaccgc cctcgtccgg ctcgcggccg
     1141 actaccgggc cgaagcgaag gacctgtcga tcgagcggat ccaccacgtc aagggcctcg
     1201 cgtggggcgc ccttctcgcc gccgagcgct tcggcccgag acacttcgag gcggaagcgc
     1261 gcgagctccg ggccgccgtc gctctctggc ccaacgaggg gccccgaaac gacgcgctcg
     1321 cggtcatcga cctctacctc gcgggcgagc tcctcccgac ggagtgaacg cggaggcgtc
     1381 cggcccccgc tcgcggcggc tctccggtcg cccgccggcg cgtcagcgcg gcaggagacc
     1441 gagcgccttc ggccagctgc cggtcggctc ccacgggatc gggtggaggg cccgctcgac
     1501 gagcgtctcg gccatctcgg tcacgatcca ccggaagctc tcctcgccga ccgagtggag
     1561 atcggcgtcg ggagcgcagt tcatgaagtc gatcgcgtag gggattccgt ccctcacggc
     1621 gaactcgacc gtgttcatgt cgtagccgag cgcgtcgcag agggcgagcg cgtccttcgt
     1681 gacccggagc gccatctcgt ccgggatgac cttgccggcg acggccgagt agcgcgcctc
     1741 gtgcggctcc ttcggcgcgt acggcatgat cctcaccttc cgccggccgg cgacccagca
     1801 gcggtagtag tcctcgaact cgatcgcttc ctgggccatc atcgtgaggt cccgcgtctg
     1861 gtcgtacgcc gagaagagtt cctcgcggct gtggaccttg tagacgtcct tccagccgcc
     1921 gccgtaggcc ggcttgagga agatcgggaa gccgagatag cggaagacct cgtcccagtc
     1981 gacgagggtc atgttccgga acgaggcgtc cgtcgtgttc ggcggatgct ccttgtgggg
     2041 caggaggacc gtccgcggca cggcgacgcc gaccgcccgg gcgacgacgt tgtcgaagaa
     2101 cttgtcgtcg gccgaccacc agaacgggtt gttgacgacc tggacgccgc gcgccgccgc
     2161 gcacttgagg aacgtccggt agaacggcac ctcgtggctg atccggtcga ggatcacgtc
     2221 gtaggcgaac gcctgctcct gggagacgtg gcccacctgc accggctcgc ccttcacggc
     2281 gaggctgcgc cgccgcgcga gctcgttgat ctcgtggcag agggcccagg ggaacgtgtc
     2341 ctccatcccg aagaggaccc cgatcgtctt cgcgttcatg cggacctcct cggatgcggc
     2401 gccgtccgtg ccccgggatc gtatcgaagc cgggggacgc cgcaaagctc cgctcccgat
     2461 agactccgcc catgaggcgc gagtaccacg cctgggtgag ccatcacctc ggccggacga
     2521 tggagttcct ctggctggga gagcggggtt acccggtcct cctcttcccc acgtcgatgg
     2581 ggcgcttctg gcagaacgag gacatgggga tcaccggcgc cctcgccgac aaggtcgacg
     2641 ccggcttcat ccagctcgtc tgcgtcgacg ccgtcgacac cgagagctgg tacaaccgga
     2701 acgctccccc cgcctaccgg gtgaggcgcc acgacgacta cgaccggtac ctccggcacg
     2761 agctcgtccc ctacgtccag caccgcgccg accgccccga cctcggggtc tacggcgcct
     2821 cgttcggcgc ctatcacgcc gccagcttcg ccgggcgcta ccccggcctc gtcacgaagg
     2881 cggtcctcct ctcggggctc taccacgtcg gccggttcct cgacggctac tgggacgacc
     2941 tctgctacta ccactgcccc gaggcgttcg tcgcgaacgc ggacgagaag accgtccggg
     3001 cgcttcgcca ggtggagtgg atcgtcgcca cgggcgagga cgacacgctc gtcggcgaga
     3061 atcgccactt cgccgggctc ctcgagggga agggcatcgg cgtccacgcc gagttctggc
     3121 ccggagtctt cggccacgac tggccgttct ggcgagaggc ggtccggcga ttcctctgag
     3181 tcgtctcctc ccggccccgc gtcccgcccg gcgggcgggc gggacgatcg cctgcaaacc
     3241 gttctcagga aacgacatcg gagccggatc ggaaacccga tccggtctcc agccgtacac
     3301 gtccgacaag gtgaggtgtc catgaccatc cgtagacaga tcctcgtcgc caccgccgcg
     3361 acggccctcc tcctcggggg acccgccgcg gcccaggaag tcaagggaga ggagcacgtc
     3421 ctcccgaacg ggatgaagct cctcctcgtc caccgccccg acgagccgtc ggtgtcggcc
     3481 ggctgggttg cgaaggtcgg cagcgcgaac gagcgcgtcg gcatcaccgg catcagccac
     3541 ctcttcgagc acatgatgtt caaggggacg aagacgctcg gctcgaagga cccgaagcgg
     3601 gacctcgaga tcatcgagga gcaggagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnncc gaagaacaag ctcgagctct ggttctggat ggagtccgac cggctgaagg
     3781 accccgtctt ccgcgagttc tacgccgagc gcgacgtcgt ctacgaggag cgtcgcctcc
     3841 gcgtcgagtc gaccccgacc ggcgagctcg acgaggcgtt cgactcggtc ttctggcact
     3901 cgacgcccta cacctggccc gtgatcggct attcctccga cgtcgcgaac atcacgaagc
     3961 agcaggccga cgactacttc gcgacctact acgccccgaa caacctgacg gccgtcctcg
     4021 tcggcgactt cgacaaggcc gaggcgctga agctcgccac cgagtacttc gggcgcatcc
     4081 cgcgcgggaa gaccgccccg cccgaggtga tcaccctgcc gacgaagtgg aaatacgatc
     4141 tccgcttcga gggggaggcc gacacgaacc cgaccgccga ggtccggtgg cggaccgtgc
     4201 ccttccagca caaggactcc tacgccctct cgatcctcgc cgagctcctg aacggccgga
     4261 cgggtcggct ctacaagtcg atggtcctcc ccgaaggcgc cgtcgccacg caggcctacg
     4321 cggtccacaa cggcttcgtc ggccccgcga agtacgccgg ctcgttcgcg atcggcgccg
     4381 aagcgaagga ggggaagctc ccggccgagg tcgcggcgaa ggccctcgcc gagatcgagc
     4441 ggctgaagac ggagcccgtc cccgccgacg agctccggaa ggtgaagaac aacgtcgcgg
     4501 ccaactcgtt ccgccgcctc tcctcgaact tcccgatcct cctccagctg atggtcaacg
     4561 aggcctacgg cgactggacg gagatcaact ccgggccgaa gaagctcgag gcggtcacgg
     4621 ccgccgacgt ccaaagggtc gcgcagacct acctcgtgaa ggagaaccag gcgaccgcgc
     4681 tcgtctcgcg caaggcgggc tcggccccct cggccgacga cgctgccctc gccgacgtcc
     4741 cggagcaggt ccgcccgatg atcaagcagg cgctccagca gatcggctcc gagaaggacg
     4801 ccgcgaagct gaaggcgagc ctcgggcaga tggagaccca ggcggcccag gttccgcccc
     4861 agatgaagaa gggcttcgac ctgctcgtga cgaaggcgcg ggagcgcctc tccgagctcg
     4921 agaaggccgc ggacgcgaag tgaggagcgg acggtgaaga cgatgaagac cctcgcgcgg
     4981 acctccgccc tcgccctcct cggcgccctc ctcctcgccc ccgcggcgca gggggtcccg
     5041 ccccaccccg acaagctgac ctacccgaag ttcacctacc agccgccgtc cgcgaaggac
     5101 taccgggtcg tcctgaagtc gggcccggtc gcctacgtcg ccgaggaccg cgagctgccg
     5161 ctcgtctcga tcaacgtcgc cctccggggc ggcacctacc tcagccccgc cgggaaggaa
     5221 gggctcgccg agctcgccgg ctacctcctc gcccgcggcg ggaccgccac gaagacggcc
     5281 gaggagctcg aggagcggct cgccttcctc gccgcctccc tcaactcggg ctacggcgcg
     5341 gaccgcggca ccgtctcgct caacctcctc gccaaggacc tcgacgaggg gctcgcgatc
     5401 ctccgcgagg tgctcacggc gccccgcttc caggacgaca agctgaagct ccgccgcgac
     5461 cagctcctca acgacatgaa gtcgcgcaac gacgacacgg ccgacatcga gcagcgcgag
     5521 cgcggcttcc tcgccttcgg cgaggggtac tacacgaacc gctacacgac gaaggcctcc
     5581 ctcgagtcga tcacccgcga ggacctcctc gccttccacc gcaagtgggt cgacccgaag
     5641 aacgtgaccg tcgccgtcgc cggcgatttc ggcaagggcg tctcccgcgc gcagatgatc
     5701 gcgaggctcg acgccctctt cgcgaagtgg ccctacaagg gagaggcggc gcctgcggtc
     5761 ccgacgccga cccacgccat ggttcccggc ctctacgtcg tcgacaagga cgtcaaccag
     5821 gggcgcgtct ccgtgatgct cccggcgctc tcgcggaccg accccgacta cgacgccgcg
     5881 ctcgtcatga acgacgtcct cggcggcggc ggcttcacga gccggatcac gaaccgggtc
     5941 cgctccgacg aggggctcgc ctactcggcc ggctccggga tcgtcggggg cgtctggtac
     6001 ccggcgacgt tccgggcctc gttccagtcg aaggcccgca cgtgcgccta cgcgacgcag
     6061 atcgtcctcg aggagatgac gaaggtccgc gaggccgagg tgacggacga ggagctccag
     6121 accgcgaaga gctcgttcat cgacacgctc tcccgcaact tcgccacgaa ggcgcagacg
     6181 gtcaacgtct tcctcgacga ggagttcacc ggacggttcg cgaaggaccc ggagtactac
     6241 cggggctacg ccgcccggat cgagaaggtg acgaaggccg acgtgcagcg cgtggcccgg
     6301 cgcctcctcg cgaccgacaa ggtcaccgtc ctcgccgtcg ggaagaagga cgagctcctg
     6361 aaccccgacc cgaagcaccc ggtgaagttc cccgacctga cgggcggaac gctgaccgac
     6421 ctgccgctcc gggacccctt cacgatgaag cccctcgccc ccgcggcgaa gtagctccac
     6481 gcacgtcccc gcgggcgacg gcccgcgccc cgcgcccccc ggcactccgg ggggcgcttc
     6541 tcttttcggc tatcgtcccg ggcgatgcgc gcgacgctcc ccgtcctccc ggtcgccctc
     6601 ctcgccctcg ccgccacgtc ccccggcccc gccctcggca cgccgccggc cgggaaggcc
     6661 gcgctgacgc tcgagcgggt ccacgccgcc gagccgctcg tcgagcccgc gccgaccggc
     6721 ctgcgctggc gcgactcgag ccgcttcacc tacctgaagc gcacgggctc cggaccgggg
     6781 gcgcaggcct cgctctggca ggaggacgcc tccacgggga agaccgagaa gctcctcgac
     6841 gcgattcccg ccgaggggac ggggaaggac ggcaagccgc ggtcgctccc gctctcagac
     6901 gcggcgtgga acgaggcggg gaccgccctc gtcgtcgccg ccgagaacga cctctggctc
     6961 tacgcgtgcg agagcgcggc ggcccgccgc ctgacctccg acgccgaagc cgaggagctg
     7021 cccgtcttct cccccgacgg cgcgaaggtc gcgtacgtga aggggaacga cctctggttc
     7081 gtcgaggtgg ggaccggccg ggtgacccgc ctgacgaacg acgggagccc gaccgtcctc
     7141 aacggacggc tcgactgggt ctacgaggag gagctcgccg gccggaacgg cgggcgggcc
     7201 tacgagtggg ctcccgactc ctccgcgatc gcgttcctcc gcctcgacgt ctcgaaggtc
     7261 cccgagtacc cgctcgtcga cttcctccca acgaacggga agcatctccc gcagcggtac
     7321 ccgaagccgg gcgaccccaa cgccgccccg tccgtgagga tcgcgacctg ggacccctcc
     7381 ggcctcgcga cgaccgtccg gtccgtctcc tacgacgggg atcgcgagct gctcggcccc
     7441 gagctcgcct ggactcccga ctcctccgcg gtcgcgttca cgaggatgga ccggacgcag
     7501 acgacgatcg aggccttcct cctcgacagg tccggcagcg ccggcccccg gagcctcctg
     7561 cgcgagacgt cggcctcctg gatcaacgcg cacgagccgc cgctcttcct ccccgacggc
     7621 tccgggttcc tctggctctc ggagcggagc ggcttcctcc acctctatcg atacggaatg
     7681 gacggctctc tcgctgggca ggtcacccgc ggcgagtggg cgatcaccga cacgccgcgg
     7741 ctcgacgccc gggcgggaac cgtcacgttc acggcgaccg aggccgaccc gcgcgagcgc
     7801 caccactacc gcgtccggct ggacgggacc ggcctgaccc ggctcacctc ccggaagggg
     7861 acccacgccg cgattccgtc ccccgacgga cgctgggtcc tcgacagctg gtcggccgtc
     7921 gacacgccga cgcgcgtcga cctcgtgcgg ggggacggaa cgctcctccg gcccgtcttc
     7981 gtcccgaaga cgtc
//
LOCUS       DAOONL010000140         7926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10736_length_7926_cov_17.252954, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000140 DAOONL010000000
VERSION     DAOONL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10736_length_7926_cov_17.252954"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PLP50_14730"
     CDS             complement(<1..1816)
                     /locus_tag="PLP50_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPA52847.1"
                     /translation="MRAPIAAIAALGLLAGAPLSGVRAEVDLARPSVATYGERDGLPQ
                     GTVHAIAFDRSGYLWVGTQDGAARFDGRSWHPFDMPDRAVSNYVRSVVGTRDGSLWFG
                     REEGGAVRRRGGVTEVFDGAAGLPAARVNHLLETADGTLWAATHGGGIARFDGARFEP
                     VGSGLPDPRVWTLLERRDASGKARLVAACEGGVAELGEGERFSPIDLGVSLAGFSVNA
                     LLETEDGGERSLWVGTFGAGLFRVRAGGVTRFGPEEGLGSRFVTSLAATGPRGSQTVW
                     VGTRDAGLYRLRGGAFEGVALGAEPLSIYALAGGGAGAPETLWVGTRLSGLLRVQPTP
                     WAPFDADSGLPGDEVFSFFETRGAEGGPEVWIGTSRGAALLRGGRLAVIGPASALPAG
                     EVRSFAEVRGPGEASEIWASLTGAGIFRREGPRWRPIEARPAFRSDDASVLLATRDEA
                     GRPVVWAGSERSGLGRFAAGRWTTLGESEGLPARSVLSLLETRTGSRRTLWAGTRGGG
                     LAEVADGRVVKIHDRTNGLPNNVVLSLAEVALPGGPRELWVGTRGGLARRSLEDPGET
                     WSLLTEESRPQLPSDVVLSIAPVPGGPVFLGTNRGVARL"
     gene            complement(1864..2580)
                     /locus_tag="PLP50_14735"
     CDS             complement(1864..2580)
                     /locus_tag="PLP50_14735"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPA52848.1"
                     /translation="MVALLGGLLGAGCPGSTGGPDPTARARAGMVDRQIVARGVKDAR
                     VLAVLREVPRHLFVPPDQAAQAYDDHPLPIGSGQTISQPYVVAFMSEQLRLTGTEKVL
                     EIGTGSGYQTAVLSRLAARVFSVEIRPELAEQAEARLAEFGATNVELRAGDGYFGWPE
                     EAPFDAILVTAAPEKVPEPLVAQLAPGGRMVIPVGGFYQELKVLERTPDGIRETSVLP
                     VRFVPFVGEAERHREGDGEP"
     gene            complement(2718..4406)
                     /locus_tag="PLP50_14740"
     CDS             complement(2718..4406)
                     /locus_tag="PLP50_14740"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA52849.1"
                     /translation="MRADVPGAHALLATLDPASAGTALLEPILEALLKGAGGARGLAL
                     DAGARCHPAARGLDPLPSELAGLAASLARGSAHVASYDQELLVRVFGTDPPEGEPPFD
                     PRVVPLTVGDETLAWVALERSPADPVGFASVAAHAASLLAWVRMSEKVREADFELKYR
                     VWELESIYDVGLSIARTLDLESLADEILMKSVSLLNARSGTLVVRPGGPADGGNAVFV
                     RSFGTPLVEPGSVEEPDGAVLVCNRREDRPASLAGAPAEKLLAVPISSEGRPLGLLVV
                     ADKENRQGGIDDFGPADVRILSLFGNQAAIALENARLHREAVEKEKMEREIEIAASIQ
                     RAILPTTLPAVSGILIAAGNRPTRQVGGDFYDVYPLPDGRVAFCVADVAGKGIPAALL
                     VSTVHACIHLLVEAGASDLTALVARTNRHLARFSSTRKFVTLFLAVYDPSDRSLRYVN
                     AGHNPGIRISAAGTELLPSGGVPIGMFESATQTESRLFLSPGDLVVLYSDGITEAVDA
                     KDEEFGMERLTRLLESGRALPPNVLKDRIFAAVEGFTRGVPQYDDQTVLVARPT"
     gene            complement(4416..4778)
                     /locus_tag="PLP50_14745"
     CDS             complement(4416..4778)
                     /locus_tag="PLP50_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPA52850.1"
                     /translation="MSETLKVTLEKPDGTVAILRTDGYINNTGGEEIARQAYTLLGEG
                     TIRLLLDLEKTKIVNSIGISILIEVLEKVLDQGGRLAFCRLTPTIEKTFQIMGLAQYA
                     RIFPAPEDALAWLRGEAE"
     gene            complement(4788..5231)
                     /locus_tag="PLP50_14750"
     CDS             complement(4788..5231)
                     /locus_tag="PLP50_14750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52851.1"
                     /translation="MSTADTREVHLTIPVAPEMEIAATAQVAALGEWMELGRDKIDEV
                     KMAVVEACINAFEHSGTPDHRVELRFRVGTERPGNGGRAYLEVDVLDNGHGFDPACVA
                     SPEIGKKLRAPHKRGWGLRIIRSLMDDVRIESGERGTMIVMRKYR"
     gene            complement(5249..5473)
                     /locus_tag="PLP50_14755"
     CDS             complement(5249..5473)
                     /locus_tag="PLP50_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52852.1"
                     /translation="MERSMGLSDRPVPGRLVGRLRAEGDRPTTVEGERRVPGLLSLLL
                     LGEAAAFAALQVSGNIPETVTVLVRALLLL"
     gene            complement(5698..7185)
                     /locus_tag="PLP50_14760"
     CDS             complement(5698..7185)
                     /locus_tag="PLP50_14760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52853.1"
                     /translation="MAVRVARSSESFDLRKKVLRLETLYDVSRALNVLRDEQALVDEI
                     VARSVALLDAERGFGVVFEEQGGPAVVSTVAFPTFPGSLAAASDPFVLDLCRARGPLS
                     RTEETVFGSPAGSVVGAPLVVRDRVLGVLVALDREAREGPAAAFDDGDRRFLESVAAL
                     AAPALDALRQLRALEADVDRLREENRALKGSLGIDDLLVGDSAGMRRVKELVVRAAAS
                     RVGVLIRGESGTGKELVARLLHAASPRRDGPFLALNCAAVPETLLEAELFGIERGVAT
                     GVEARQGKFELATGGTIFLDEIADAPLAIQAKLLRVLQEREIERLGGRRRIPVDVRVV
                     AATNADLERSIREKRFREDLYYRIRVVEIPLPPLRERREDIPRLIGHFLSRIAAREGR
                     RPKSLAREAVARLLDYPFPGNVRELENLLEGAVALVPGETIEAGDLQLRGGRPGPGSN
                     GSQDLATLELEHIRRVLESVSGNKSEAARILGINRRTLYRKHVSN"
     gene            complement(7187..>7926)
                     /locus_tag="PLP50_14765"
     CDS             complement(7187..>7926)
                     /locus_tag="PLP50_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52854.1"
                     /translation="GRYAEAEEHLKRALEIKPDFLPAVGHLGTVEACRGNAEGAVTWY
                     RRASELAETWKDRYSFAGAAVMALLDAGRAEDALVLLDEVAVAAPAREGVDALVLRWN
                     VARSRAIVLAIAGRVEEAEAELAGLPELPPDFDPKGKAALETSLSGPKAAMAVARGDL
                     EGAVRHFREILATEAERGMGDFSYYPSIFRVRTRLADALVTLGRPDEAREELRPVLAV
                     NPRFAPALEVLARIGGRSSATTGGAGR"
BASE COUNT         1142 a         2947 c         2791 g         1046 t
ORIGIN      
        1 cgaggcgcgc gacgccccgg ttcgtgccga ggaagacggg accgccgggg accggcgcga
       61 tcgagaggac gacgtcgctc ggaagctgcg gccggctctc ctcggtgagg agcgaccagg
      121 tttcgcccgg gtcttcgagg ctccggcgcg cgagacctcc gcgagtcccg acccagagct
      181 cgcgcggacc gcctggcaag gccacctcgg cgagcgagag aacgacgttg ttcggaagcc
      241 cgttcgtccg gtcgtggatc ttcacgaccc ggccgtccgc cacctcggcg aggccgccgc
      301 cgcgcgtccc ggcccacagc gttcggcggg agcccgtgcg cgtctcgagg agcgagagga
      361 cggagcgagc cgggaggccc tcgctctccc cgagggtcgt ccagcggccc gccgcgaaac
      421 ggccgagccc ggacctctcg ctcccggccc agacgacggg gcgcccggct tcgtcccgcg
      481 tggcgaggag gacgctcgcg tcgtcggagc ggaaggccgg ccgcgcctcg atcggacgcc
      541 atcgggggcc ctcccgacgg aagatccccg cgccggtcag gctcgcccag atctccgacg
      601 cttctcccgg gcctcggacc tcggcaaacg agcggacctc gccggcggga agcgccgacg
      661 ccggcccgat caccgccagc cgtccgccgc gaaggagggc ggccccacgg ctcgtgccga
      721 tccagacctc gggcccaccc tccgcgcctc gggtctcgaa gaacgagaag acctcgtcgc
      781 ccggcaggcc cgaatccgca tcgaacggcg cccacggcgt cggttgaact cggaggagcc
      841 cggagaggcg cgtcccgacc cagagggtct ccggcgctcc ggcgccgccg ccggcgagcg
      901 cgtagatcga gagcggctcc gccccgagcg ccacgccctc gaacgctcct ccccgcagcc
      961 ggtagaggcc cgcgtcgcgg gtcccgaccc agaccgtctg cgagccgcgg gggccggtcg
     1021 ccgcgaggct cgtgacgaaa cggctcccga ggccctcctc cggtccgaag cgcgtgacgc
     1081 ccccggcgcg cacgcggaag aggccggctc cgaacgtccc gacccagagc gagcgctccc
     1141 cgccgtcttc cgtctcgagg agcgcgttca cgctgaaccc ggcgagcgac acgccgaggt
     1201 cgatcggcga aaagcgctcc ccttcgccca gctccgccac gcccccttcg caggccgcga
     1261 cgaggcgcgc ctttcccgac gcatcccgcc gctccaggag ggtccagacc ctcggatcgg
     1321 gaagcccgct cccgaccggc tcgaacctcg ccccgtcgaa gcgggcgatc cctccgccat
     1381 gagtcgccgc ccagagcgtc ccgtccgcgg tctcgaggag atggttgacc cgtgcggcgg
     1441 ggaggccggc ggcgccgtcg aagacttcgg tcacgccgcc ccggcgccgg accgcgcctc
     1501 cttcctcgcg accgaaccag aggctcccgt cccgcgtccc gacgacggac cgcacgtagt
     1561 tcgagacggc ccggtcgggc atgtcaaacg ggtgccagct ccgtccgtcg aaccgcgccg
     1621 cgccgtcctg cgtgccgacc cagaggtatc cgctgcgatc gaacgcgatc gcgtggaccg
     1681 tcccctgcgg gagcccgtcc ctctctccgt aggtcgcgac cgaggggcgg gcgaggtcga
     1741 cctccgcgcg gacgcccgaa agcggcgcgc cggcgaggag acccagcgcc gcgatggctg
     1801 cgatcggtgc gcgcatgctc gaacgagact atcgtgacgc cgcggtcgga gggagcgtcg
     1861 gactcaaggc tcgccgtctc cctcgcggtg ccgctcggcc tccccgacga acgggacgaa
     1921 ccggaccggg aggacgctcg tctcccggat cccgtcggga gtcctctcga ggaccttcag
     1981 ctcctggtag aagcctccga ccgggatcac catccgccct cccggcgcga gctgggcgac
     2041 gaggggctcg gggaccttct ccggcgccgc cgtgacgagg atggcgtcga agggggcctc
     2101 ctccggccat ccgaagtagc cgtcgccggc gcgcagctcg acgttcgtcg ccccgaactc
     2161 cgccagccgg gcctcggcct gctcggcgag ctcgggccgg atctcgaccg agaagacgcg
     2221 cgcggcgaga cgggaaagga cggcggtctg gtagccggag ccggtcccga tctccaggac
     2281 cttctccgtc ccggtcagac ggagctgctc gctcatgaag gcgacgacgt agggctggga
     2341 gatcgtctgg ccgctcccga tcggaagggg atggtcgtcg taggcctgcg ccgcctggtc
     2401 cggcgggacg aaaaggtgcc gcgggacctc ccggaggacc gcgaggacgc gggcgtcctt
     2461 cacaccccgg gcgacgatct gccgatcgac cattcccgcg cgggcgcggg ccgtcgggtc
     2521 ggggcctccg gtcgagccgg ggcatcccgc gccgaggagc ccgccgagga gggccaccac
     2581 gctcccgatt ctccacccgg cggccggcga cgcgtgcgac gtcctcacga aggagcattc
     2641 tagcgatcgc cgtgccgcgc cgaccgtcct gaaacgggaa cgccgccggg cccccggcgc
     2701 cgcggcccgg cgggacctca ggtcggacgc gcgacgagga ccgtctggtc gtcgtactga
     2761 ggaacgcccc gggtgaagcc ctcgaccgcg gcgaagatcc ggtccttcag gacgttcggc
     2821 ggcagcgcgc gccccgactc gaggaggcgc gtcagccgct ccatcccgaa ctcctcgtcc
     2881 ttcgcgtcga ccgcctccgt gatcccgtcg gaatagagga cgacgaggtc gcccggagaa
     2941 aggaagagcc ggctctccgt ctgcgtcgcc gactcgaaca tcccgatcgg gactccgccg
     3001 ctcggcagga gctccgtccc ggctgccgag atccggatgc cggggttgtg tcccgcgttc
     3061 acgtagcgga ggctccggtc cgaagggtcg tagaccgcga ggaagagcgt cacgaacttc
     3121 cgcgtggagg agaagcgggc gaggtggcgg ttcgtccggg cgacgagggc cgtcaggtcg
     3181 gacgccccgg cctcgacgag gaggtggatg caggcgtgga cggtcgagac gaggagcgcc
     3241 gcagggatcc ccttccccgc gacgtcggcc acgcagaacg cgacgcgtcc gtccgggagc
     3301 gggtagacgt cgtagaagtc tccgccgacc tgccgcgtcg gccggttccc ggcggcgatc
     3361 aggatgccgg agacggccgg gagcgtcgtc gggaggatcg cccgctggat ggaggcggca
     3421 atctcgatct cgcgctccat cttctccttc tcgaccgcct cgcggtgcag gcgcgcgttc
     3481 tcgagcgcga tggcggcctg gttcccgaac agcgagagga tcctcacgtc ggccgggccg
     3541 aaatcgtcga tccctccctg ccggttctcc ttgtcggcga cgacgaggag gccgagcgga
     3601 cgtccctccg aggagatcgg gaccgccagg agcttctcgg ccggcgcccc cgcgagcgac
     3661 gcgggacggt cctcgcgccg attgcagacg aggacggccc cgtcgggctc ctccaccgag
     3721 cccggctcca cgagcggggt cccgaagctc cggacgaaga cggcgttccc gccgtccgcc
     3781 gggccgccgg gccggacgac gagcgtcccg ctccgcgcgt tcaggagcga gaccgacttc
     3841 atgaggatct cgtcggcgag cgactcgagg tcgagggtcc tcgcgatcga gaggccgacg
     3901 tcgtagatgc tctcgagctc ccagacccgg tacttcagct cgaagtccgc ctcgcggacc
     3961 ttctccgaca tccgcaccca ggcgaggagg gaggcggcgt gcgccgcgac ggacgcgaag
     4021 ccgaccgggt cggccgggga gcgctcgagg gcgacccagg cgagcgtctc gtcgccgacg
     4081 gtcagcggga cgacgcgcgg gtcgaagggg ggctctccct cgggagggtc cgtcccgaag
     4141 acccggacga ggagctcctg gtcgtagctc gcgacgtggg cgctgccccg cgccaggctc
     4201 gcggcgagcc cggcgagctc cgacgggagc gggtcgaggc cgcgggcggc gggatggcag
     4261 cgggcccccg cgtcgagcgc gaggcctcgg gcgcctcccg cccccttcag gagcgcttcg
     4321 aggatcggct cgagaagagc ggtcccggcc gaggccggat cgagcgtcgc gaggagcgcg
     4381 tgggcccccg ggacgtcggc gcgcatcggc ccgcgctact ccgcctcacc gcgcagccac
     4441 gccagggcgt cctccggcgc ggggaagatc cgcgcgtact gcgcgagccc catgatctgg
     4501 aacgtcttct cgatcgtcgg cgtcaggcgg cagaacgcga ggcgtccccc ctggtccagg
     4561 accttctcga gcacctcgat caggatcgag atcccgatgg agttcacgat cttcgtcttc
     4621 tcgaggtcga gcaggaggcg gatcgttccc tcgccgagga gcgtgtaggc ctgccgggcg
     4681 atctcctctc cgccggtgtt gttgatgtac ccgtccgtcc ggaggatcgc gaccgtcccg
     4741 tcgggcttct ccagggtcac tttcaacgtc tcgctcatcg tcgcccttca gcggtacttc
     4801 cgcatcacga tcatcgtccc gcgctcgccg ctctcgatcc tcacgtcgtc catcaggctc
     4861 cggatgatac gaagccccca ccccctcttg tgcggtgccc ggagcttctt cccgatctcg
     4921 ggcgacgcga cgcacgccgg gtcgaagccg tgaccgttgt cgaggacgtc gacctccagg
     4981 taggcgcggc cgccgttccc gggtctctcg gtgccgaccc ggaacctcag ctccacgcgg
     5041 tggtcggggg tcccgctgtg ctcgaacgcg ttgatacacg cctcgacgac ggccatcttc
     5101 acttcgtcga tcttgtcccg tccgagctcc atccattcgc ccagcgccgc gacctgggcc
     5161 gtcgcggcga tctccatctc cggggcgacc ggaatggtga ggtggacctc tcgggtgtct
     5221 gccgtcgaca tcggcggtgt tcggcgcgtc agagcagcag gagagcccgg acgagcacgg
     5281 tcacggtttc ggggatgtta cccgacacct ggagcgccgc gaacgccgca gcctcgccga
     5341 gcaggaggag ggagagcagg cccgggacac ggcgctcgcc ctcgacggtg gtgggtcggt
     5401 cgccctcggc gcggagacgc ccgacgagcc gccccggcac cggccgatcc gacagtccca
     5461 tgctgcgttc catcgactcc tcccgcgacg gaatgacgca acagctgcgc cagaacgacc
     5521 ctgccgagga tattctatct aaacaccttg tttacagcta cttacaacaa cgtccccgat
     5581 cgtcgaccga agggatagcg gcataatgcc acatcccgtt cgaatcgaaa gttcgcagtc
     5641 acacaaacag ctctaatgca attgcttacg cggcatggga cataatgccc cggtgtccta
     5701 attagacacg tgcttccggt agagagtccg gcggttgatg ccgagaatgc gcgcggcctc
     5761 gctcttgttc ccgctgaccg actcgaggac tctgcggatg tgctcgagct cgagcgtcgc
     5821 caggtcctgg gagccgttcg accccggccc cggacgtcca ccgcgaagct gcaggtcgcc
     5881 ggcctcgatc gtctctcccg gcacgagcgc caccgccccc tcgagcaggt tctcgagctc
     5941 gcggacgttt cccgggaacg ggtagtcgag caggcgcgcg accgcctcgc gcgcgagcga
     6001 tttcggccgc cgaccctcgc gcgccgcgat ccgcgacagg aagtgcccga tgagacgtgg
     6061 gatgtcctcc cgccgctcgc gaagcggagg aagcgggatc tcgacgaccc ggatccggta
     6121 gtagaggtcc tcgcggaacc gcttctcccg gatcgaccgc tcgaggtccg cgttcgtcgc
     6181 cgccacgacg cggacgtcga ccgggatccg ccgccgcccg ccgagccgtt cgatctcccg
     6241 ctcctgaagg acccggagca gcttggcctg gatcgcgagc ggggcgtcgg cgatctcgtc
     6301 gaggaagatc gtcccgcccg tggcgagctc gaacttcccc tgccgcgcct cgacccccgt
     6361 cgccacgccc cgttcgatgc cgaagagctc ggcttcgagg agcgtctccg gcaccgccgc
     6421 gcagttgagg gccaggaacg gcccgtcgcg gcgcggggag gccgcgtgga gaagccgggc
     6481 gacgagctct ttgcccgtgc cgctctcgcc gcggatcagg acgccgacac gggacgcggc
     6541 cgcgcgcacg acgagctcct tgacccgccg catcccggcc gagtcgccca cgagcaggtc
     6601 gtcgatcccg agcgagccct tcagcgcccg gttctcctcc cgaaggcggt ccacgtcggc
     6661 ctcgagggca cgcagctggc ggagggcgtc gagcgccggc gccgcgaggg ccgcgaccga
     6721 ctcgaggaaa cgccggtcgc cgtcgtcgaa cgccgccgcc gggccttccc gggcctcccg
     6781 gtcgagagcg acgaggacgc cgaggacgcg gtcccgcacg acgagcgggg ccccgacgac
     6841 gctccccgcg gggcttccga agacggtctc ctccgtccgg gagagggggc cgcgggcccg
     6901 gcagaggtcg aggacgaacg ggtcggacgc ggcggcgagg cttcccggga acgtcgggaa
     6961 cgcgaccgtc gagacgacgg ccgggccgcc ctgctcctcg aagacgaccc cgaagccgcg
     7021 ctcggcgtcg aggagggcca cggagcgcgc cacgatctcg tcgacgaggg cctgctcgtc
     7081 ccgcagcacg ttcagcgccc gcgagacgtc gtagagggtc tcgaggcgga ggaccttctt
     7141 gcggaggtcg aacgactcgc tggagcgcgc gacccggacc gccatctcag cgcccggcgc
     7201 ctcccgtcgt cgcggaggaa cgcccgccga tccgggcgag gacctcgagc gccggcgcga
     7261 acctcgggtt cacggccaga acgggccgca gctcctcccg cgcctcgtcc ggccgcccca
     7321 gcgtgacgag cgcgtccgcg aggcgggtcc tcacgcggaa gatcgacggg tagtacgaga
     7381 agtcgcccat cccgcgctcc gcctcggtcg cgaggatctc ccggaagtgc cggacggccc
     7441 cctcgaggtc tccccgggcg acggccatcg ccgccttcgg tcccgaaagg ctcgtctcga
     7501 gagcggcctt gcccttcggg tcgaaatcgg gcggcagctc cggcaggccc gcgagctccg
     7561 cctccgcctc ctcgacgcgc ccggcgatcg cgaggacgat cgcccgggag cgggccacgt
     7621 tccagcggag cacgagcgcg tccacgccct cccgggcggg agcggcgacg gcgacctcgt
     7681 cgaggagaac gagcgcgtcc tcggcgcgcc ccgcgtcgag gagcgccatg acggcggcgc
     7741 cggcgaagga gtagcggtct ttccacgtct ccgcgagctc ggaggcgcgc cgataccagg
     7801 tcaccgcgcc ttccgcgttc ccgcggcagg cctcgacggt ccccaggtgg ccgacggccg
     7861 ggaggaagtc cggcttgatc tcgagcgccc gcttcaggtg ctcctccgcc tcggcgtagc
     7921 ggcccg
//
LOCUS       DAOONL010000141         7863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10858_length_7863_cov_20.656122, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000141 DAOONL010000000
VERSION     DAOONL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_7863_cov_20.656122"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1063)
                     /locus_tag="PLP50_14770"
     CDS             complement(59..1063)
                     /locus_tag="PLP50_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HPA52855.1"
                     /translation="MSSEGTDRTRPVRPKDVPAGYLDSTGRADLFAGGVRMIPVETPA
                     GTFRVWTKRVGNNPRVKLLLLHGGPGFTHETFEACDTRLPAAGVEYYYYDQLGSYYSD
                     QPDRPELWELPRFVDEVEQVRRALGLGRESFFLLGQSWGGILALEYALRHQEHLKGLV
                     ISNMMSSIPAYNAYARDVLAPRMDAAALAEIRRLEADGRTDDARYMELLGPHYYERHV
                     LRIPMDGWPEPLVRSFRHMNPKVYVPMQGPSELGASGKLESWDRSADLGAIRVPTLVL
                     GAAHDTMDPGHLRWMAGAVARGRYHHCPDGSHLAIYDDQERYFEGLIRFLRDVDEGVF
                     "
     gene            1285..2316
                     /locus_tag="PLP50_14775"
     CDS             1285..2316
                     /locus_tag="PLP50_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPA52856.1"
                     /translation="MTQSGGVLAALALALLAAFGCSAGSRRPATFAEAAREKADVTIV
                     VTDSGLGGLSVAADLASRLPASGLVRSARIVFVNALLDDAIGYNDLRDEADKVRVFDA
                     ALEAMEKRYAPDLILVACNTLSVFYERTLHAKRGTTETVSIVPMGVDLVEKALREKPE
                     ARAVLFATKGTIDSNVHRRLLERRGIAADRIVGQACPKLTGAIERGAHDAETASRIEG
                     FVKEAVGRLGDRPGPVVASLNCTHFGYARPLWEEAFEKLGHPGVTVLDPNPLMTDHVL
                     RAGGERRHPETAVTVEVVSKTPIGEDVKGPLGALLRTVSPATADALARYAHVPDLFHV
                     EIDPAAILR"
     gene            2373..3524
                     /locus_tag="PLP50_14780"
     CDS             2373..3524
                     /locus_tag="PLP50_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52857.1"
                     /translation="MPRRAGILAPLLLLALPAAGAEPSFRTELSIGASWESIETHRPL
                     VVLGGTPSAGYDYSADEKARTLEVAATRWFGGVTDDGATPLALLPYVARASRVSARVA
                     AGGTSRDSFGRSSGQLATYEVWYAGDASLREAQLSGEWFIGRGIALRGGLEHGRERET
                     AASTSLESPSGRAEVATATTRTKVTSGTLGASLRLGEHEVSISGVYGASDRSREDATV
                     FTGGSLPLFSRLTTDGLTRRGRVAARLLLLRRRLAVDLSGEYALATSSSDLDTTLSGP
                     YAKGRTIGRRATLEATWYPTRRLEVGAGLAYETRDASSGSARALRPSSEETTRTLGFA
                     ARWFATERAAVTFDASRSEAESISPPGGSTYQRFDETTTRLVLGAAVRF"
     gene            3528..5519
                     /locus_tag="PLP50_14785"
     CDS             3528..5519
                     /locus_tag="PLP50_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPA52858.1"
                     /translation="MGESPFLRDLVIVLAASLPVLFVFQKLRVPTIVAFLFTGILIGP
                     YALGLVGDPDQVRKIAELGVVLILFYVGLGFSLPRLKELGRTAVVSGALQMTLAVLAT
                     AGVLLLFGQDLRPAIFGGLLVAGSSTAVVLPVLSARDEIDAPFARQFLGTSLFQDLGV
                     IPLILLLPALAGGAQAGEAPSLLSVLGRVGLAAASVAVLVVVARFVVPPLLDRLVRVA
                     GRETFTAGVVVVILGMVAIAVEAGVSAAMGAFAAGIVVAESESMADVSATFAPFRDIL
                     TSLFFVSIGMLLDPGFVLSRPLLVLGVVVAVVLLKVVTAYPALRAARAVPRVAIRAAF
                     SLATVGEFSFVLALAGLGYGLLQEGPQQLFVAVSVLTMLSAPLLVAAGARLVGLFPER
                     LEEADTPNASVLRSGHVVVVGYGLNGSNVARALGEIGIPYVVLDAEADRVSRARAAGV
                     EVLLADATSPTALEIAGIARARALVVTVPDPAASRKIVRMARSRNTGVKTIVRTRYIR
                     EVDELRRAGANEVIPEEFETSIEILARLLRSFHVPGNLVATQIRVLRDEAYRKLRDPQ
                     ARPETGRRLSALMAAGTSDLVLVLPEMAASGRTLGELDLEADHVAVPALLRDGAPLAP
                     PPRDLPIEPGDTLLLVGAHEDLVRATKKLEGAGPADPDA"
     gene            complement(5551..6015)
                     /locus_tag="PLP50_14790"
     CDS             complement(5551..6015)
                     /locus_tag="PLP50_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPA52859.1"
                     /translation="MPVRKLKEFLDQNGVRWVSIVHSPAYTAQEVAASAHVKGKELAK
                     TVMVKVDGKPVMIVLPASQRVDFQVLREVTGGQNVVLASEAEFRELFPDCEAGAMPPF
                     GNLYGMDVYVAPKLAEDEEIAFNAGTHTELMRLHYADFERLVKPKIAAFAQR"
     gene            complement(6206..7435)
                     /locus_tag="PLP50_14795"
     CDS             complement(6206..7435)
                     /locus_tag="PLP50_14795"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA52860.1"
                     /translation="MTPAALLLAALALAGLVITSLPVVLLERLRQRRRLFSRGPRGRA
                     SAPSPRAASTPGRPHLSILKPLKGLDDGLDENLASFAALRGIDYEVVLSVADADDPAL
                     DVVDRVRARHPEAPFVLVVGGAPEGRFGNPKVERLVAASRVARGDLFLVSDSNVRVAP
                     DAVADTVALLDDPTVGCVSNLFVGAGARDLGAVVESLHLLTFVVPGNVLAAWGGVPCV
                     VGKSMALPRSVHDSIGGFAAFGDVLAEDQAIGLAVKEAGFRVLLSPVVVENVVERRSL
                     RRALDRQVRWGKIRFAFSKALFAGEVLMNPFPLALLSTLLALAGGSPWTGPLASFALL
                     ALLVRLLQASFLARLCGAPLPLAGLLAVPLKDLLQLATQVVPFVSREVVWHGHRARIG
                     KGTTLLETGSRVRLAGA"
     gene            complement(7432..>7863)
                     /locus_tag="PLP50_14800"
     CDS             complement(7432..>7863)
                     /locus_tag="PLP50_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52861.1"
                     /translation="DEFVSADGPVEEALARARDAGAVTVACHPNEQSEWFANTFYLWN
                     RKDAMKELVHLWELACRWDLFPEVGRSGFAFLGNSDFHRAPHLWSWKTLLDCEKTPGA
                     ILAALRKGRGLGLTRLHAPAPAPALGPEPCEGLSALARTSA"
BASE COUNT         1033 a         2799 c         2941 g         1090 t
ORIGIN      
        1 tcgccgtcgc ggtcgccttc ggacgcgcgt cggagagcgg acgcgaggaa gcggcgagct
       61 agaagacgcc ctcgtcgacg tcccggagga accggatcag cccctcgaag tagcgctcct
      121 ggtcgtcgta gatcgcgagg tggctcccgt cggggcagtg gtggtaccgg ccgcgggcga
      181 cggcccccgc catccagcgc aggtgccccg ggtccatcgt gtcgtgcgct gcgccgagga
      241 cgagggtcgg gacccggatc gcgcccaggt ccgcggagcg gtcccagctc tcgagcttcc
      301 cggaggcgcc gagctcgctc ggcccctgca tcgggacgta gaccttcggg ttcatgtggc
      361 ggaacgagcg gaccagcggc tcgggccacc cgtccatcgg gatcctgagg acgtgcctct
      421 cgtagtagtg agggccgagg agctccatgt agcgcgcgtc gtcggtccgg ccgtccgctt
      481 cgagccgccg gatctcggcg agcgcggcgg cgtccatccg cggcgcgagg acgtcgcgcg
      541 cgtaggcgtt gtacgccggg atgctcgaca tcatgttcga gatgacgagc cccttcaggt
      601 gctcctggtg gcgcagggcg tactcgaggg cgaggatccc gccccacgac tgcccgagga
      661 ggaagaacga ctccctcccg agtccgaggg cgcggcggac ctgctccacc tcgtcgacga
      721 agcgcggcag ctcccagagc tccggccggt ccggctggtc gctgtagtac gacccgagct
      781 ggtcgtagta gtagtactcg acgccggccg ccgggagccg ggtgtcgcac gcctcgaaag
      841 tctcgtgcgt gaagcctggg ccgccgtgga ggaggaggag cttcacccgt gggttgttgc
      901 ccacgcgctt cgtccagacg cggaacgtcc cggcgggcgt ctcgacgggg atcatccgga
      961 cgcccccggc gaagaggtcc gcgcgacccg tggagtcgag atagcccgcg gggacgtcct
     1021 tcggcctgac gggccgcgtg cggtccgttc cttcgctcga catggggcct cctgcgtcgg
     1081 tcggcgacgt tccccccgcc gtggagcggg gcgttggccg gacccgggaa tcggaaatcc
     1141 aacggacgat acggcaggaa cgccgcgctg gcgagcggga gatcggacgg cgggagatcg
     1201 tgtcgcggga gacccgtgcc ggtcgcgcgc cgtcgcgggc gggtccagaa tccgcggggc
     1261 ggcggccggc cgcgggaggg gttgatgacg cagtccggag gagtcctcgc agcgctcgct
     1321 ctcgccctcc tcgccgcctt cggctgctcg gccgggagcc gcaggccggc cacgttcgcc
     1381 gaggcggcgc gggagaaggc cgacgtgacg atcgtcgtga ccgactcggg tctcggcggc
     1441 ctctccgtgg cggcggacct cgcgagccgc ctccccgcga gcggcctcgt ccgctcggcg
     1501 cggatcgtct tcgtgaacgc cctcctcgac gacgcgatcg gctacaacga cctgcgcgac
     1561 gaggccgaca aggtgagggt cttcgatgcg gcgctcgagg cgatggagaa acggtacgcc
     1621 cccgacctga tcctcgtcgc ctgcaacacg ctgtcggtct tctacgagag gaccctccac
     1681 gcgaaacgcg ggacgacgga gaccgtcagc atcgtcccga tgggcgtcga cctcgtcgag
     1741 aaggcgctcc gcgagaagcc ggaggcgcgg gcggtcctct tcgcgacgaa ggggacgatc
     1801 gactcgaacg tccaccgccg cctcctcgag cggcggggaa tcgccgcgga ccggatcgtg
     1861 ggccaggcct gcccgaagct gaccggcgcg atcgagcggg gcgctcacga cgcggagacc
     1921 gcctcgcgga tcgagggctt cgtgaaggag gcggtcggcc ggctcggcga tcgccccggg
     1981 ccggtcgtcg cgagcctgaa ctgcacccac ttcggctacg cgcggccgct ctgggaggag
     2041 gcgttcgaga agctcgggca tccgggcgtg acggtgctcg acccgaaccc cctcatgacg
     2101 gaccacgtgc tccgcgcggg cggcgagcgg cggcacccgg agacggcggt cacggtcgag
     2161 gtcgtctcga agacgccgat cggcgaggac gtgaaggggc cgctcggcgc gctgctgcgc
     2221 acggtctctc ccgcgacggc cgacgccctc gcgcgctacg cgcacgtgcc ggacctcttc
     2281 cacgtcgaga tcgaccccgc cgcgatcctc cgctgagcgc atcgttcccg gagcggcgcc
     2341 ggggcgcggg acatcggcta acctcggacg gaatgccgag acgcgccggg atcctcgcgc
     2401 ccctgctcct gctggcactg cccgcggccg gggcggagcc atccttccgg acggagctct
     2461 cgatcggcgc ctcatgggag tcgatcgaga cccaccggcc gctcgtcgtc ctcggcggga
     2521 ccccttccgc gggctacgac tactcggccg acgagaaggc ccgcacgctg gaggtcgcgg
     2581 cgacccgctg gttcggcggc gtgacggacg acggggcgac gcccctcgcg ctccttccct
     2641 acgtcgcgcg cgcctcgagg gtctcggccc gcgtcgcggc cggcggaacg agccgcgact
     2701 cgttcgggcg gtcctcgggg cagctcgcga cgtacgaggt ctggtacgcc ggcgacgcct
     2761 ccctccgcga ggcgcagctc tcgggcgagt ggttcatcgg ccggggcatc gcgctgcggg
     2821 gcgggctcga gcacgggagg gagcgggaga cggcggcctc gacatcgctc gagagcccct
     2881 cggggcgcgc cgaagtcgcg acggcgacga cgcgcacgaa ggtgacgtcg gggacgctcg
     2941 gcgcctcgct ccggctcggc gagcacgagg tgagcatctc gggggtctac ggcgcctccg
     3001 accggagccg cgaggacgcg acggtcttca ccggtgggtc cctgccgctc ttctcgcgcc
     3061 tgacgaccga cgggctcacg cgccgggggc gcgtcgcggc ccggctcctc cttctccgga
     3121 ggaggctcgc cgtcgatctc tcgggggagt acgcgctcgc cacctcctcc tcggacctcg
     3181 acacgacgct ctccggtccg tacgcgaagg ggcggacgat cggccgccgg gcgacgctcg
     3241 aggcgacgtg gtacccgacg cgccggctcg aggtgggcgc gggtctcgcg tacgagacgc
     3301 gggacgcctc gtccgggtcg gcccgggcgc tccggccctc gagcgaggag acgacgagga
     3361 cgctcggctt cgccgcgcgc tggttcgcga cggagagggc ggcggtcacg ttcgacgctt
     3421 cgcggtcgga ggccgaatcg atctcgccgc ccggcgggtc gacgtaccag cggttcgacg
     3481 agacgacgac gcgtctcgtc ctcggcgcgg cggtgaggtt ctaggaggtg ggagagagcc
     3541 cgttcctccg cgacctcgtc atcgtcctcg cggcgtcgct gccggtcctc ttcgtcttcc
     3601 agaagctgcg cgtgccgacg atcgtggcgt tcctcttcac ggggatcctg atcggaccgt
     3661 atgcgctcgg cctcgtcggc gaccccgacc aggtccggaa gatcgccgag ctcggcgtcg
     3721 tcctgatcct cttctacgtc gggctcggct tctcgctccc gcggctgaag gagctcggga
     3781 ggacggccgt cgtgagcggc gcgctccaga tgacgctcgc cgtcctggcc accgcggggg
     3841 tcctcctcct cttcggccag gacctccggc cggcgatctt cggcggcctc ctcgtcgcgg
     3901 gctcctcgac cgcggtcgtc ctgccggtcc tctccgcgcg ggacgagatc gacgccccgt
     3961 tcgcccgcca gttcctcggg acctcgctct tccaggacct cggcgtcatc ccgctcatcc
     4021 tcctcctccc ggcgctcgcc ggcggggcgc aggcgggcga ggcgccgtcg ctcctttccg
     4081 tcctcggacg ggtcggcctc gccgccgcga gcgtcgcggt cctcgtcgtc gtggcgcgct
     4141 tcgtcgtccc gccgctcctc gaccggctcg tccgggtcgc cggccgggag acgttcacgg
     4201 ccggcgtcgt cgtcgtgatc ctcgggatgg tcgcgatcgc cgtcgaggcg ggggtctccg
     4261 ccgcgatggg ggcgttcgcg gccgggatcg tcgtcgccga gagcgagtcg atggccgacg
     4321 tgagcgcaac gttcgcgccg ttccgcgaca tcctgacgag cctcttcttc gtctcgatcg
     4381 ggatgctcct cgacccgggc ttcgtcctgt cgcgtccgct cctcgtcctc ggcgtcgtcg
     4441 tcgccgtcgt cctgctgaag gtcgtcacgg cctacccggc gctccgggcg gcccgcgccg
     4501 tgccgcgcgt cgcgatccgg gccgccttct cgctcgcgac ggtcggggag ttctccttcg
     4561 tcctcgcgct cgcggggctc ggctacgggc tcctccagga agggccgcag cagctcttcg
     4621 tcgcggtgtc ggtcctcacg atgctctcgg cgccgctcct cgtcgcggcg ggggcgcgcc
     4681 tcgtgggcct cttcccggag cgcctcgagg aggccgatac gccgaacgcc tcggtcctcc
     4741 gctcgggcca cgtcgtcgtc gtcggctacg gcctgaacgg gagcaacgtg gcccgcgcgc
     4801 tcggcgagat cgggatcccg tacgtcgtcc tcgacgccga ggccgaccgc gtctcccgcg
     4861 cccgtgcggc gggggtggag gtcctcctcg ccgacgcgac ctcgccgacc gcgctcgaga
     4921 tcgccgggat cgcgcgggcc cgcgcgctcg tcgtcacggt ccccgacccg gccgcgagcc
     4981 ggaagatcgt ccggatggcc cggtcccgga acaccggggt gaagacgatc gtcaggacgc
     5041 ggtacatccg cgaggtcgac gagctgcgcc gcgccggcgc caacgaggtg atcccggaag
     5101 agttcgagac ctcgatcgag atcctcgccc ggctgctccg ctcgttccac gtcccgggga
     5161 acctcgtcgc gacgcagatt cgcgtcctcc gggacgaggc gtaccggaag ctgcgcgatc
     5221 cgcaggcccg gcccgagacg gggcgtcgcc tctcggcgct gatggccgcg gggacctccg
     5281 acctcgtcct cgtcctcccc gagatggcgg cgtccgggag gacgctcggc gagctggacc
     5341 tcgaggcgga ccacgtggcg gtcccggccc tgctacgcga cggggcgccg ctcgcgccgc
     5401 ctccccggga cctgccgatc gagccgggcg acacgctcct cctcgtcggc gcgcacgagg
     5461 acctcgtccg cgccacgaag aagctcgaag gggccggccc agccgatccg gacgcctgat
     5521 cggaccggcg agcgggcggg cggaccggcc ctaccgctgc gcgaacgccg cgatcttcgg
     5581 ctttacgaga cgttcgaagt ccgcgtagtg gagcctcatc agctccgtgt gcgttcccgc
     5641 gttgaacgcg atctcctcgt cctccgcgag cttcggcgcc acgtagacgt ccatcccgta
     5701 caggttcccg aacgggggca tcgcgcccgc ctcgcagtcg gggaagagct cgcggaactc
     5761 cgcctcgctc gcgaggacga cgttctgccc gcccgtcacc tcgcgcagga cctggaagtc
     5821 gacgcgctgc gacgcgggga ggacgatcat cacgggcttg ccgtccacct tcaccatgac
     5881 ggtcttcgcc agttctttcc ccttgacgtg cgcggaggcg gcgacctcct gggccgtgta
     5941 ggccggggag tggacgatcg atacccagcg gacgccgttc tggtcgagga actccttcag
     6001 cttccggacg ggcatgggcc cctcctttcc ggccgggcgg ggcacgcccc ggccgacccg
     6061 tacgcgggcg ctcgcgggga ctcggtgtgg tgcggcgcgc tcatgccgtc acctccgaaa
     6121 ggggttcggt acggggaatt ctatgcccgc gcgacggagg gaaacgaggg ccgcggcggc
     6181 ccgtgtcgcg gatcggccga ccgcctcagg ctccggcgag acggacgcgg gacccggtct
     6241 cgaggagcgt cgtccccttt ccgatccgcg cccggtggcc gtgccagacg acctcccgcg
     6301 agacgaaggg gaccacctgc gtcgcgagct gaaggaggtc cttgagcggg acggcgagga
     6361 ggccggcgag gggaagcggc gcgccgcaga gccgggcgag gaagctcgcc tggaggaggc
     6421 ggacgaggag cgcgaggagg gcgaaggagg cgagcggccc ggtccaggga gagcctcccg
     6481 cgagcgccag gagcgtggag aggagggcga gcgggaaggg gttcatcagg acctcccccg
     6541 cgaagagcgc cttcgagaac gcgaagcgga tcttccccca gcggacctgg cggtcgagag
     6601 cccgccgcag gctgcgccgc tcgacgacgt tctcgacgac gaccggggag aggaggacgc
     6661 ggaagccggc ctccttgacc gcgaggccga tcgcctggtc ctccgcgagg acgtcgccga
     6721 acgcggcgaa cccgccgatc gagtcgtgga cgctccgcgg gagggccatc gacttgccga
     6781 cgacgcaagg gacgccgccc caggcggcga ggacgttccc cgggacgacg aaggtcagga
     6841 ggtggaggct ctccacgacg gcgccgaggt cgcgcgcgcc ggccccgacg aagaggttcg
     6901 agacgcagcc gacggtcggg tcgtcgagga gagcgacggt gtcggcgacc gcgtcggggg
     6961 cgacccggac gttcgagtcg gagacgagga agaggtcgcc gcgggcgacg cgcgaagcgg
     7021 cgacgagacg ctccaccttc gggttcccga acctcccctc cggggcgccg ccgacgacga
     7081 ggacgaacgg ggcctccgga tgccgggcgc ggacgcggtc gacgacgtcg agcgcggggt
     7141 cgtcggcatc ggcgacggag aggacgacct cgtagtcgat tccccggagc gcggcgaacg
     7201 acgcgaggtt ctcgtcgagg ccgtcatcga gccccttcag cggcttgagg atggagaggt
     7261 gcggacggcc cggcgtggaa gcggcccgcg gggaaggagc ggaagctctg ccgcgcgggc
     7321 cgcgggagaa aaggcgcctc cgctggcgga ggcgctcgag gaggaccacc ggcagcgagg
     7381 tgatcacgag gcccgcgagg gcgagcgccg cgaggaggag ggcggcgggc gtcacgcgga
     7441 ggtcctcgcg agcgcggaga gcccctcgca cggctcgggc ccgagcgcgg gggccggagc
     7501 cggagcgtgg agccgcgtga gcccgagtcc gcgtcccttc cggagcgcgg cgaggatcgc
     7561 ccccggcgtc ttctcgcagt cgaggagcgt cttccaggac cagaggtgcg gagcgcggtg
     7621 gaagtcgctg ttgccgagga acgcgaagcc cgaacggccc acctcgggga agaggtccca
     7681 gcggcaggcg agctcccaga ggtggacgag ctccttcatc gcgtccttcc ggttccagag
     7741 gtagaaggtg ttcgcgaacc actcggactg ctcgttcgga tggcaggcga ccgtgacggc
     7801 gccggcgtcc cgggccctcg cgagcgcctc ctcgacgggt ccgtcggcgg agacgaactc
     7861 gtc
//
LOCUS       DAOONL010000142         7847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10885_length_7847_cov_21.055313, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000142 DAOONL010000000
VERSION     DAOONL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_7847_cov_21.055313"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLP50_14805"
     CDS             complement(<1..1184)
                     /locus_tag="PLP50_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HPA52862.1"
                     /translation="MKVLMISANRERDPSPVFPLGLAYLAGPVERAGHETSLLDLCFA
                     EDVPEVVRGALERMRPDVVLVSIRNLDNVTWPVCRSYVDGVREVVALCRDTAPVVLGG
                     SAFSLEPESFLEATGARCGVVGEGEEVIGPLLERLAAGLPLAGLPGVVEAGGDGWTAP
                     RAPSAVGAPSRGSFDVSRYLTEGGAANVQTKRGCPFTCVYCTYPVLEGSRVRARPAAD
                     VVRELRTLVDEHGVDYVEFCDDIFNFPEAAARELCEAMIADGLRLSWSAFVNPGHLDA
                     RLVDLMARAGCDAVEIGTDSGSAAMLRSLGKSFTVDDVRAASRLCRDAGLATAHYLLF
                     GGPGENEATVAESVALMDELAPEAVIVMVGIRVYAGTELHATAVSEGVVRADQCLLEP
                     VF"
     gene            complement(1212..2501)
                     /locus_tag="PLP50_14810"
     CDS             complement(1212..2501)
                     /locus_tag="PLP50_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52863.1"
                     /translation="MGSRILFVLPGWPKGRLWGEMSFRFPSLSLAVVAAATPEKWETA
                     LHDEGFGPVDLDDPADVVALTGMTAQATRAYEIADAFRARGKTVVMGGFHASNLPAEA
                     LQHVDAVVVGEAESVWPKLLGDLGEGKLQKVYRPDGPVDTAAIPVARRSIFDGKGYLL
                     TNTVQTTRGCPYDCEFCSVTAYFGRKYRKRPVEAVLAELAEMKKRGSYAFFVDDNIVV
                     DRNYSLRLFRGMKGLGMKWLSHASLDLAEDPELLLAMGEAGCIGLFVGFESLDEKALA
                     AMGKKTNHVATYLDAVKAFRDSGIGILGSFVLGLDGDGPDVFERTLRFCETARLPAAI
                     FPILTPYPGTKVRERLLAEDRILSSDWRNYDMEHVNFRPKGMTVDELQAGYELLMKEF
                     YSFGSMWRRLGWHRSLPAFGPMNFGFRGALRRKVADA"
     gene            complement(2586..3632)
                     /locus_tag="PLP50_14815"
     CDS             complement(2586..3632)
                     /locus_tag="PLP50_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52864.1"
                     /translation="MPEGPSPRLTLHHDPPARRPRGILPYDFAKVGFFLFGIPRNGFG
                     SIDVSKECNLRCSHCYFFEGGEGEAPAYAGRAELTVDEWIAKLERLKRSTSRLGFPFF
                     QCTWVGGEPLIRKELIERGRRYFKYNTVVTNGTIPLPDWPDVSWCLSIDGDEETHERI
                     RRRKGIYRRAMQNVAERPNLSVTVAYCITRANVHCLEQAVIDWSRAGARDMTFDFYTP
                     IGDMCDGNDELFLPLAERDVVLDKLLALKAIYGEFFVLPERAFRMMKSDVCRKVTDSC
                     LFSRKSFAFAPDGVQKEKCMMGPKADCDRCGCVVPFYLASLTDRSRILRDLGGELSHA
                     ARRVARGLFTAVPG"
     gene            3773..4495
                     /locus_tag="PLP50_14820"
     CDS             3773..4495
                     /locus_tag="PLP50_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52865.1"
                     /translation="MRAVLGALAGLAGAAAGGLIATILAAVFAKAANVTTREGAAGYL
                     VVAVGILGALAGLVVGVVLYARSAPAGEGPSAAGSALLGLLGVVVVVAAILWAWTLSH
                     EGPASYGDTLASLELELRVKKADAPAGPPSSWLDVEVQTAKTRPAAVVLSDKVREEGD
                     GIVVPLVQNPLHRATGRLVVVRVADRHVEVFSPRWKAKPDPKADWSPWIAPRLAEKTG
                     GEGGEAVRPILELRYRVRLYGD"
     gene            4549..5898
                     /locus_tag="PLP50_14825"
     CDS             4549..5898
                     /locus_tag="PLP50_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPA52866.1"
                     /translation="MRDHASARRRAHLERIRVAALAAVEPARAVARLLSLEDGVLRAG
                     DARLPLASDASVRVVAAGKAAAAMTEAAAAALGDRLERGVLVTKHGHLDGRTIPPGVR
                     SFEAGHPVPDETGREAVAAVERLLSGSRKGDVALALLSGGASALLADPAEPLTLPDLG
                     ETTRLLLRSGATIVQLNAVRKHLSRLKGGQLARLAAPATVVSLILSDVVGDPLDVIAS
                     GPTAPDPTTFEDAWEIVDRLGLVDALPSAVCARLRAGRAGKLPETPKPGDPLFELVRN
                     EIVGSNRLAALAATEEARGLGYDALLLSTFVEGEAREVARVVAALGRSVLAHGDPVPP
                     PACLVLGGETTVTVRGNGRGGRNQELALAAALALEGIEGISVMALATDGSDGPTDSSG
                     AIVDGETARAIRAAGIDPVLALAANDAYPALEAGGAQLFTGPTGTNVNDLTVVLVTG"
     gene            complement(5911..6672)
                     /locus_tag="PLP50_14830"
     CDS             complement(5911..6672)
                     /locus_tag="PLP50_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52867.1"
                     /translation="MRESPYRSRPGTRLSARGTHRGVAVLIAGLLVMGGISVRTSAAS
                     GASGRETNASVPDVNGGRVGMAERRAAAARTLEAWLAERGELPGARVTPLGIEIPGWV
                     FFRVSYPPRPGLEELGETHGYHVVREDGTVVSGPVEEELSKLFRAEGLANGKLVRTPE
                     TLAQAVLVLAGKGGLVASDGDASELARRFEGAELAGPRLERTADGVVFVFFAQEEAGP
                     ERAPLSFLAVRVVVDSGGRASLTLRSIPVRFRARR"
     gene            6793..>7847
                     /locus_tag="PLP50_14835"
     CDS             6793..>7847
                     /locus_tag="PLP50_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52868.1"
                     /translation="MPESITALELSALVSRVFQPTSEDTRLGIMVDLPDEKVPDDAGW
                     AARRAIAAEWAELLGMQARETGYETRLLLYPNVHTNNGDLPGRCWIHAGGPVPASAAE
                     LDSAASVAMTEVFDTFPMLLALTKFSSTAPLKMAAKKHPMRAATMPGFSAAMIPALRL
                     DYGEVNRRVDILKAHLDRATGADFVFRHPGGEDRLHLDLRHRTGHASGGLLPKRGVAG
                     NLPSGESYIVPYEGEVDGDPSRSEGVLPVQFGTEVVRYHVVGNRAVEVLTRGPESERE
                     AALLAKEPAYANLAELGLGVLAAFGVKPVGSVLLDEKLGLHIAFGRSEHFGGQVGPSA
                     FSSPEAVVHIDRVYVPET"
BASE COUNT         1131 a         2930 c         2742 g         1044 t
ORIGIN      
        1 tagaagaccg gctcgaggag gcactggtcg gcccggacga cgccttcgct cacggcggtc
       61 gcgtggagtt ccgtcccggc gtagacgcgg atgccgacca tgacgatgac ggcctcgggc
      121 gcgagctcgt ccatcagggc gacgctctcg gcgaccgtgg cctcgttctc gcccgggccg
      181 ccgaagagga ggtagtgggc ggtggcgagc ccggcgtcgc ggcagaggcg cgacgccgcg
      241 cgcacgtcgt cgacggtgaa cgacttcccg aggctccgga gcatcgccgc ggagccggag
      301 tcggtgccga tctccaccgc gtcgcacccg gcccgggcca tcaggtcgac gagccgggcg
      361 tcgaggtgcc ccgggttgac gaacgccgac caggagaggc gcaggccgtc cgcgatcatc
      421 gcctcgcaga gctcgcgcgc cgcggcctcc gggaagttga agatgtcgtc gcagaactcg
      481 acgtagtcga cgccgtgctc gtcgacgagc gttcggagct cgcggacgac gtccgcggcg
      541 gggcgggcgc gcacgcggct cccctcgagg accggatacg tgcagtagac gcaggtgaac
      601 gggcagccgc gcttggtctg gacgttcgcg gcgcccccct cggtcaggta gcgcgagacg
      661 tcgaagctcc cccggctcgg cgcgccgacc gccgacggcg cgcgcggcgc cgtccacccg
      721 tcgccgcccg cctcgacgac gccggggagg cccgcgagcg ggaggccggc cgcgaggcgc
      781 tcgaggagcg gcccgatgac ctcctcccct tcgccgacga cgccgcagcg cgcgccggtc
      841 gcctcgagga acgactccgg ctccagggag aacgcggagc cgccgaggac gaccggggcc
      901 gtgtcgcggc agagcgcgac cacctcgcgc acgccgtcga cgtaggagcg gcagaccggc
      961 caggtgacgt tgtcgaggtt ccggatcgag acgaggacga catcgggccg catccgctcg
     1021 agcgccccgc ggaccacctc cggcacgtcc tcggcgaagc agaggtcgag gaggctcgtc
     1081 tcgtgccccg cccgctcgac gggaccggcg aggtaggcga ggccgagcgg gaagacggga
     1141 gaggggtccc tctcccggtt cgccgagatc atcaggacct tcacgccggg aacgcccttc
     1201 cgccgcggcc ctcaggcatc ggccaccttg cgcctcagag cgccccggaa gccgaagttc
     1261 atcggcccga aggccgggag cgaccggtgc cagccgagcc gcctccacat cgagccgaac
     1321 gagtagaact ccttcatcag gagctcgtag cccgcctgga gctcgtccac cgtcatcccc
     1381 ttcggccgga agttgacgtg ctccatgtcg tagttccgcc agtcgctcga gaggatccgg
     1441 tcctcggcga ggaggcgctc gcggaccttc gtcccgggat acggcgtgag gatcgggaag
     1501 atcgccgccg gaaggcgcgc cgtctcgcag aagcggagcg tccgctcgaa gacgtcgggg
     1561 ccgtcgccgt cgagaccgag gacgaaggag ccgaggatgc cgatcccgga atcccggaac
     1621 gccttcaccg cgtcgaggta ggtggcgacg tggttcgtct tcttccccat cgccgcgagc
     1681 gccttctcgt cgagcgactc gaagccgacg aagaggccga tgcagccggc ctcccccatc
     1741 gcgaggagca gctcggggtc ctcggcgagg tcgagcgagg cgtgcgagag ccacttcatc
     1801 ccgaggccct tcatcccgcg gaagagcctc agcgagtaat tgcggtcgac gacgatgttg
     1861 tcgtcgacga agaaggcgta ggagccgcgc ttcttcatct ccgcgagctc cgcgaggacc
     1921 gcctcgaccg ggcgcttgcg gtatttccgc ccgaagtagg ccgtcaccga gcagaactcg
     1981 cagtcgtacg ggcagccgcg cgtcgtctgg acggtgttcg tcaggagata gcccttgccg
     2041 tcgaagatcg accgccgcgc gaccgggatc gccgccgtgt cgaccggccc gtcgggccgg
     2101 tagaccttct gcagctttcc ctccccgagg tcgccgagga gcttcggcca gacggactcg
     2161 gcctcgccga caacgacggc gtcgacatgc tggagcgcct cggcgggcag gttgctcgcg
     2221 tggaagcccc ccatgacgac ggtcttcccg cgcgcgcgga acgcatcggc gatctcgtag
     2281 gcccgggtcg cttgggccgt catccccgtg agggcgacga cgtcggccgg gtcgtcgagg
     2341 tcgaccggcc cgaagccctc gtcgtggagg gcggtctccc acttctccgg ggtcgccgcg
     2401 gcgacgaccg cgagcgagag gctcgggaag cgaaacgaca tctctcccca gagacgccct
     2461 ttcggccagc cgggcaggac gaagaggatg cgcgacccca cggtcgcgca ttctccacga
     2521 gacgggaccg cgcgcgccac cccggacccc ggcggggtcc ctcgacccgt ccgggcgcgc
     2581 ggccctcagc ccgggaccgc ggtaaagagc ccccgggcga cccgccgtgc ggcgtgggag
     2641 agctcgcccc cgaggtcccg caggatgcgg ctccggtcgg tcagcgacgc caggtagaac
     2701 ggcacgacgc agccgcagcg gtcgcagtcc gccttcggcc ccatcatgca tttctccttc
     2761 tgcacgccgt cgggggcgaa ggcgaaggac ttcctcgaga agaggcagct gtcggtgacc
     2821 ttccggcaga cgtccgactt catcatccgg aaggcccgct cggggaggac gaagaactcc
     2881 ccgtagatcg ccttcagggc gaggagcttg tcgaggacga cgtcgcgctc cgcgagcggc
     2941 aggaagagct cgtcgttccc gtcgcacatg tcgccgatcg gcgtgtagaa gtcgaacgtc
     3001 atgtccctgg caccggcgcg ggaccagtcg atgacggcct gctcgaggca gtggacgttc
     3061 gcccgggtga tgcagtaggc gacggtcacg ctcaggttcg gccgctcggc gacgttctgc
     3121 atggcccgcc ggtagatccc cttccggcgc cggatccgct cgtgcgtctc ctcgtcgccg
     3181 tcgatggaga ggcaccagct cacgtcgggc cagtcgggaa gggggatcgt gccgttcgtg
     3241 acgacggtgt tgtacttgaa gtacctccgc ccgcgctcga tcagctcctt gcggatgagc
     3301 ggctcgccgc ccacccaggt gcattggaag aacgggaagc ccaggcgcga ggtgctccgc
     3361 ttcagccgct cgagcttcgc gatccactcg tcgacggtga gctccgcccg gccggcgtag
     3421 gcgggcgcct ccccttctcc cccctcgaag aaatagcagt gcgagcagcg gaggttgcac
     3481 tccttggaga cgtcgatcga gccgaagccg ttccgcggga tgccgaagag gaagaagccg
     3541 accttcgcga agtcgtacgg gaggatcccg cgcggacggc gggccggcgg gtcgtgatgg
     3601 agggtcagcc tcgggctcgg tccttcaggc acgggttcca ggaggatgtc atccgcaggg
     3661 gccgatcgac aagcctcgcg accgcccggc actgtggcag gatcacggcg atgccgtcgg
     3721 tcgagacggg ccgctccgac cggaacgcgg ccgccaccgc gaggaacctc gcgtgagggc
     3781 tgtcctcggg gccctcgccg gactcgcggg cgccgccgcc ggcggcctga tcgcgacgat
     3841 cctcgcggcg gtcttcgcga aggcggcgaa cgtcacgacc cgcgaggggg ccgccgggta
     3901 cctcgtcgtc gccgtcggga tcctcggggc gctcgcgggc ctcgtcgtcg gcgtcgtcct
     3961 gtacgcgagg tccgccccgg ccggtgaggg accgagtgcc gccggctccg ccctgctcgg
     4021 cctcctcggt gtcgtcgtcg tcgtcgcggc gatcctgtgg gcctggacgc tctcgcacga
     4081 agggcccgcg agctacggcg acaccctcgc gtcgctcgag ctcgagctgc gcgtgaagaa
     4141 ggcggatgcg ccggccgggc ccccgtcgag ctggctcgac gtcgaggtcc agacggcgaa
     4201 gacgcggccg gccgccgtcg tcctctcgga caaggtgcgt gaggagggtg acggaatcgt
     4261 cgtccccctc gtccagaacc cgctccaccg cgccaccggc cgcctcgtcg tcgtccgcgt
     4321 ggcggaccgg cacgtcgagg tcttctcgcc ccgctggaaa gcgaagccgg acccgaaggc
     4381 cgactggagc ccctggatcg cgccgcgcct cgcggagaag acgggcggcg aaggcggcga
     4441 ggccgtgcgg ccgatcctgg agctccgcta ccgcgtccgc ctctacggcg attgaccgcc
     4501 gcgccggaga acggagcgaa gggcccgccc cggcgcacaa tgccgcgcat gagggaccac
     4561 gcgagcgctc gccgccgggc tcacctcgag aggatccgcg tcgccgcact cgcggccgtc
     4621 gagccggcga gagcggtcgc gcggctcctc tcgctcgaag acggcgtcct tcgggccggc
     4681 gacgcccgcc ttccgctcgc cagcgacgct tccgtccggg tcgtcgccgc cggcaaggcg
     4741 gccgcggcca tgaccgaagc cgcggcggca gccctcggcg atcggctcga gcgaggtgtc
     4801 ctcgtcacga agcacgggca cctcgacggt cggacgattc cccccggggt gaggagcttc
     4861 gaggccggtc acccggtccc cgacgagacg ggtcgcgagg ccgtcgccgc cgtcgagagg
     4921 ctcctctcgg gaagccgcaa gggcgacgtc gccctggcgc tcctctcggg cggcgcctcg
     4981 gcgctcctcg cggacccggc cgagccgctc accctcccgg acctcggcga gacgacccgc
     5041 ctgctgcttc gctccggcgc gacgatcgtc cagctgaatg ccgtgaggaa acacctttcg
     5101 cggctcaagg ggggacagct cgcacgtctc gccgcgcccg ccaccgtcgt ctcgctgatc
     5161 ctctccgacg tcgtcggcga cccgctcgac gtcatcgcgt cgggtccgac cgcccccgac
     5221 ccgacgacgt tcgaggacgc gtgggagatc gtcgatcggc tcgggctcgt cgacgccctg
     5281 ccatcggcgg tctgcgcgcg tctgcgggcc ggcagggccg ggaagctccc ggaaacgccc
     5341 aagccgggcg acccgctctt cgagctcgtc cgaaacgaga tcgtcggctc gaaccggctc
     5401 gcggccctcg cggcgacaga ggaggcgcga ggcctcggct acgacgcgct cctcctctcg
     5461 acgttcgtcg agggggaggc gcgcgaggtc gcccgcgtcg tcgccgctct cggccgcagc
     5521 gtcctcgccc acggcgaccc ggtgcccccg cccgcctgtc tggtcctcgg cggcgagacg
     5581 accgtgacgg tgcgcgggaa cggtcgcggc ggccggaacc aggagctcgc gctcgcggcc
     5641 gcgctcgcgc tcgaaggaat cgaggggatc tccgtgatgg cactcgccac cgacggctcc
     5701 gacggcccga ccgactcctc cggcgcgatc gtcgacggcg agacggcgcg ggcgattcga
     5761 gccgcgggca tcgacccggt cctcgcgctc gccgcgaacg acgcctatcc cgcgctcgag
     5821 gcgggaggcg cccagctctt caccggcccg accgggacga acgtcaacga cctcaccgtc
     5881 gtcctcgtca cgggctgatt ccgcgacccc tcagcgacgg gcccggaacc ggaccgggat
     5941 cgaccggagc gtgaggctcg cgcgaccgcc tgaatcgacg acgacgcgca ccgcgagaaa
     6001 cgagagcggg gcgcgttcgg gcccggcctc ctcctgcgcg aagaagacga aaacgactcc
     6061 gtccgccgtc cgctccagac gcggcccggc aagctcggcc ccctcgaagc gtcgagcgag
     6121 ctcggaggcg tcgccgtccg aggcgacgag cccccctttc cccgcgagga cgaggaccgc
     6181 ctgggcgagc gtctcgggcg tccgcacgag cttcccgttc gccagcccct ccgcgcggaa
     6241 gagcttcgag agctcctcct ccacgggacc ggacacgacg gtgccgtcct cgcgaacgac
     6301 gtgatagcca tgcgtttcgc ccagctcctc gagccccggt ctgggtgggt acgagacccg
     6361 gaagaacacc catccgggga tttcgattcc gagaggagtc acccgcgcgc cgggaagctc
     6421 cccgcgctcc gccagccagg cctcgagggt cctcgccgcc gccgcccgtc tctccgccat
     6481 cccgacacgc cctccgttca catccggcac cgaggcattc gtctcccgtc cggacgcccc
     6541 gctcgcggcc gacgtccgga cggagatccc gcccatgacg agcagcccgg caatcaggac
     6601 cgcgacaccc cggtgcgtcc cgcgagctga cagccgggtc ccggggcgcg accgatacgg
     6661 gctctccctc acgaggtccg ccagtctacc gcccgacggt ggcacaccgg cgcgtcggcc
     6721 tcgcccggcg cgggcctcgc ccggtcgaca cccccgcacg cttcgggcac catcccatcc
     6781 gaggaggccc ccgtgcccga atcgatcacc gccctcgagc tctccgccct cgtctcccgc
     6841 gtcttccagc cgacctccga ggacacccgc ctcgggatca tggtcgacct ccccgacgag
     6901 aaggtccccg acgacgccgg ctgggcggcg cgccgcgcga tcgcggccga gtgggcggag
     6961 ctcctcggga tgcaggcccg cgagaccggg tacgagacgc gcctcctcct ctacccgaac
     7021 gtccacacga acaacggcga tctccccggc cgctgctgga tccacgccgg cggacccgtc
     7081 ccggcgagcg ccgcggagct cgactcggcg gcgtcggtcg cgatgacgga ggtcttcgac
     7141 accttcccga tgctcctcgc gctgacgaag ttcagctcga cggcgccgct gaagatggcc
     7201 gcgaagaagc acccgatgcg cgccgcgacg atgcccggct tttccgccgc gatgatcccg
     7261 gccctccgac tcgactacgg cgaggtgaac cgccgcgtcg acatcctgaa ggcgcacctc
     7321 gaccgggcga ccggcgccga tttcgtcttc cgccatcccg gcggcgagga tcgccttcac
     7381 ctcgacctgc gccaccggac gggccacgcc tccggcggac tcctcccgaa gcgcggcgtc
     7441 gcggggaacc tcccctcggg cgagtcgtac atcgtcccct acgagggcga ggtcgacggc
     7501 gacccgagcc ggtcggaagg tgtcctcccg gttcagttcg gcacggaggt cgtccggtac
     7561 cacgtcgtcg ggaacagggc ggtcgaggtc ctgacgcgcg gccccgaatc ggagcgcgag
     7621 gcggcgctcc tcgcgaagga gcccgcctac gcgaacctcg ccgagctcgg cctcggcgtc
     7681 ctcgcggcgt tcggcgtcaa gccggtcggc tccgtcctcc tcgacgagaa gctcggcctc
     7741 cacatcgcgt tcggccggag cgagcacttc ggcgggcagg tcggcccgag cgccttctcc
     7801 tcgcccgagg cggtcgtcca catcgaccgc gtctacgtcc ccgagac
//
LOCUS       DAOONL010000143         7813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_10956_length_7813_cov_16.550013, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000143 DAOONL010000000
VERSION     DAOONL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10956_length_7813_cov_16.550013"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..279)
                     /locus_tag="PLP50_14840"
     CDS             complement(4..279)
                     /locus_tag="PLP50_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPA52869.1"
                     /translation="MKLYVGNLSYNTTDATLNDLFAPFGQVESARVITDRDSGTSKGF
                     GFVEMSNSDAQKAMGALNGREIDGRAIRVNEAKPQENRGGGGGRGRW"
     gene            complement(543..1736)
                     /locus_tag="PLP50_14845"
     CDS             complement(543..1736)
                     /locus_tag="PLP50_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52870.1"
                     /translation="MDHARRSAPRFLAVAALLAFPALPAFASGPAPTPPPAASDGRWF
                     DRGMELHEEEKWDEAIAAFRRAIETGERVGASSFNVACAYSRKGEKELAFEWLGRAEE
                     AGFELRRHLDDDDLDPLRTDPRFTALKAKARAARSGRSAEKCRAAVVRYDQLAGRGSK
                     DGRALFDNGQDLLSCGELDRAARTFMAAAEAGRREGTALYNAACARALESRNAEALDL
                     LERAVAAGYDGTGHLRRDDDLDSLRAEPRFARIVKDAEALSLGDFPSLGARLLRSQMV
                     AEADEAAARFEQYLRTNPRSGRAWWSIGRVRLAAGQDREAVHAFTRALSLGYRRGVTA
                     YDLACAHARLKERDAAFARLFEAIDLGAATADHVEEDDDLWNLRRDPRFEKAIARARG
                     ARARR"
     gene            1916..3523
                     /locus_tag="PLP50_14850"
     CDS             1916..3523
                     /locus_tag="PLP50_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HPA52871.1"
                     /translation="MGQVSSPVPGEFDVVVIGGAIAGASTAILLRRRRPGLRVLVVEK
                     SDAFDWKVGESTVEISAYFLTRVLKLYDHLSREHLPKQAFRYWFHNEKVTCLREASEV
                     GPTQLARTPGFQLDRAVLDEHVLKLAAEEGSEVWRPARVVDVSLGGEAGSSVTVEKAD
                     GTRVTVRAKWVVDASGRQALLARKRGGIRPVEGHPTSSIWVRYRGVKDLDGKEVVGTD
                     PADPFVRSVVASRRLATNHFTGWGYWVWFIPLRGGETSVGLVWDTRLVRPEGSTPLEK
                     LTSVLSANPLTREMLENAEPVEGDCRYYGTLPYFLDRFIDHGWTCVGDAGGFLDPFYS
                     PGLDQMSFSVHMRVELILKALAGAPREEMAKEYEDHNRKYARFFEYFYEAIYRDKYHL
                     MGDYDTMTTAFLMDTALYYLVAVQPVYTESAGRLGIPPYYGDGAEVGLVPMRFYQQRL
                     VSIAKRKKALGIYGNHNAGRRPGFVGFSLRSSAHVMLWHGLLRWWKAELANALTYVWK
                     PRPLEGPQPVLPPRPAEEAGAEAPARG"
     gene            3529..4338
                     /locus_tag="PLP50_14855"
     CDS             3529..4338
                     /locus_tag="PLP50_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52872.1"
                     /translation="MPGARSGRLLAPLAAGAILLAGALPARAWDVDALVLEAAARQRE
                     GEVAAGRIALERVEVETELDAGGRRKTSERRRYVVVMDRRGLAARELLEVDDRAATSA
                     EREEARRQDEKRKRAALEPGSARSDEEELLSGRVPLLDLYHRLDFRLLGEERIDGRPT
                     FVVAFRPKPGLVARTIRDRVLNAFAGTAWIDVAEIQVRKIDGRLTRPVRVLGGALLEV
                     KGVRLLYETREVLPGLWAPCREEILITATAAMVLPFRRELRFEFSSYRPAT"
     gene            complement(4454..4795)
                     /locus_tag="PLP50_14860"
     CDS             complement(4454..4795)
                     /locus_tag="PLP50_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPA52873.1"
                     /translation="MGDGAWVKELKVGITVCDRDGTALEMNDRAAETFAADGGRDLVG
                     KSILDCHPPRARELFEVLLRTGGTNAYTIEKNGVKKLIYQAPWYENGVCRGIVELSLP
                     IPFDLPHFVRS"
     gene            complement(4873..5517)
                     /locus_tag="PLP50_14865"
     CDS             complement(4873..5517)
                     /locus_tag="PLP50_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52874.1"
                     /translation="MRAARVVLVVAAFAAACGPQAKKPEAPEAPAPAPQVGEGAAPAA
                     RSEAASDPCAVLRAPMTRHDAAGSPLAFSFEMPEGFVPKDFSKGETVHVDVTRDLDGD
                     RADEYVLRFAYPTKGVAGPEKLVAVWRKLPATVKVLEKSVGGRTMYVQRTKMGEMSGF
                     VALYPIAGNSTGAYSVLGGLTAAPKPCRDEAAEVLERMLLSFEPNPSIGPIPQG"
     gene            complement(5582..6037)
                     /locus_tag="PLP50_14870"
     CDS             complement(5582..6037)
                     /locus_tag="PLP50_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPA52875.1"
                     /translation="MRSVSRDIVLNASPDRVFTAFTDVPEVLAWLADAAVIGPRVGGN
                     WALGWYADEDSEDGYHVLGTVEAFEPPSRLVVRDLRFSTPEGELLEGMRLSIEIASEN
                     GATRVSLRHEGVGEGASWDGYAAGLGPSWERSLADLRGWLEEGRKLPGR"
     gene            complement(6048..6995)
                     /locus_tag="PLP50_14875"
     CDS             complement(6048..6995)
                     /locus_tag="PLP50_14875"
                     /inference="COORDINATES: protein motif:HMM:NF039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HPA52876.1"
                     /translation="MIAGRERPELLRWGLVSIGGVWGSSLALPALLSAGWLAAERKEP
                     ARRLVLWAQKWLLQGLAFFLLPVYHRSAAYTPGQAAFMALLAAAALAATIDVVYDDVV
                     APRRPLHGALLAFLAFATVNVTLPMVWGTGGLWSLGASGLLATAVFVSFVASSGRDRR
                     LGRAALVGALFLALVTLGRPAVPPAPLRLVSARVGRALAPNGLDVASPQSSLRGGPGR
                     RLFAVAAVFAPAGLEEGVRHVWALDGRVFFRSRVIGITGGRREGFRSRSGVTVGRLAP
                     GQRLRLDVETAHGQLVGRVELVVTGALPRDQPSSGAEPL"
     gene            complement(7202..>7813)
                     /locus_tag="PLP50_14880"
     CDS             complement(7202..>7813)
                     /locus_tag="PLP50_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52877.1"
                     /translation="AVLLERHVPGYREIVASELPFFVREAAGLVFGRKLAETTARRCL
                     RAAAALWVDARTREDLVMPTCLFAMEYLRWLAEDSESWDETKEHESRTTREEELIRET
                     SSAAFSALCAERPGERWPVLQGLVDAEDATRLLLARELLLEEKREFVDLRIEGWLRTL
                     PEALARELRREWARLRELGKDDLELDAIERLVALETLRGSRGA"
BASE COUNT         1091 a         2807 c         2821 g         1094 t
ORIGIN      
        1 ggactaccag cggccgcgcc cgccgccgcc gccgcggttc tcctgcggct tggcctcgtt
       61 gacgcggatg gcgcggccgt cgatctcgcg gccgttgagg gcgcccatcg ccttctgcgc
      121 gtcggagttc gacatctcga cgaagccgaa gcccttcgac gtgccgctgt cgcggtcggt
      181 gatgacgcgg gcggactcga cctggccgaa cggcgcgaag aggtcgttga gggtggcgtc
      241 ggtcgtgttg taggagaggt ttccaacgta gagcttcatc gtgagtcctc gtgtcttgtc
      301 tcggtcgtcc ggagacgatc tggtggagag ataggtgagt cggaagcgcg cgggggacgg
      361 ggcctgctcg ttacgctttg ccgcgccgtc tgcccgaagt tcggagagcg aggaggcgga
      421 gagtggcgaa tgggccaggt tcagagtacc acgacttcgc gggagggcgg cgcgaggccc
      481 ctcggtggac ggcgccccgg tggcgcgccg tccgttcccg gccggaaggg agccggggcc
      541 cctcagcgcc gggcccgggc tccccgcgct ctcgcgatcg ccttctcgaa gcgggggtcg
      601 cgccggaggt tccagaggtc gtcgtcctcc tcgacgtggt cggcggtggc cgcgccgagg
      661 tcgatcgcct cgaacaggcg ggcaaacgcg gcatcgcgct ccttcagccg cgcatgggcg
      721 caggcgaggt cgtaggccgt gacgccgcgt cggtagccga gggaaagggc ccgggtgaag
      781 gcgtggacgg cctcccggtc ctggccggcg gcgagacgga ccctgccgat gctccaccac
      841 gcccgcccgc tcctcgggtt cgttcgcagg tactgctcga accgcgccgc ggcctcgtcg
      901 gcctccgcga ccatctggga acgcaggagc cgggccccga ggctcgggaa gtctccgagg
      961 gagagcgcct cggcgtcctt cacgatcctc gcgaagcgtg gctcggcgcg gagagagtcg
     1021 aggtcgtcgt cccgccgcag gtgtccggtt ccgtcgtagc cggcggcgac ggcgcgctcg
     1081 aggaggtcga gagcctccgc gttccggctc tcgagcgcgc gggcgcaggc cgcgttgtag
     1141 agcgcggtcc cctcccgccg gccggcctcc gcggccgcca tgaaggtccg ggcggcgcgg
     1201 tcgagctccc cgcacgaaag gaggtcctgc ccgttgtcga agagcgctcg gccgtccttt
     1261 gacccccgcc ccgcgagctg gtcgtagcga acgacggccg cccggcactt ctcggcgctc
     1321 cgccccgacc gggccgcccg cgccttcgcc ttcaacgcgg tgaaccgggg atccgtgcgg
     1381 agcggatcga ggtcgtcgtc gtcgagatgg cgccgcagct cgaacccggc ctcttcggcc
     1441 cgcccgagcc actcgaaggc gagctccttc tcgcccttgc gcgagtacgc gcaggcgacg
     1501 ttgaacgacg aggccccgac gcgctcgccc gtctcgatcg cgcggcggaa cgcggcgatc
     1561 gcctcgtccc acttctcctc ttcgtggagc tccatccccc tgtcgaacca gcggccgtcg
     1621 ctggcggccg ggggcggcgt cggggccggc cccgacgcga acgccgggag cgcggggaag
     1681 gccaggagcg cggcgacggc gaggaaccgc ggagcggagc gtcgggcgtg gtccatactc
     1741 gctggatacg gagcggcggg gacggtgttt cccgaacgag gcccccgacc cgatcggtct
     1801 ggcccggctt cgttcggcgc aagtgcttgc agcgtagccg gttcgacatc cttcgtgggg
     1861 tgcggccccc attcaatatc atccgcccat gaatcggacg gaaaaggttc cgcaagtggg
     1921 acaggtctct tcgcccgttc cgggcgagtt cgacgtcgtc gtgatcgggg gcgcgatcgc
     1981 cggcgcttcg accgcgatcc tcctccgccg ccgcaggccg ggtctccgcg tcctcgtcgt
     2041 ggagaagtcg gacgcgttcg actggaaggt gggggagtcg accgtcgaga tctccgccta
     2101 cttcctcacg cgcgtcctga agctctacga ccatctctct cgcgagcacc tgccgaagca
     2161 ggccttccgc tactggttcc acaacgagaa ggtcacctgt ctgcgcgagg cgtccgaggt
     2221 cggccccacg cagctcgcgc ggactcccgg gttccagctc gaccgggccg tcctcgacga
     2281 gcacgtcctg aagctcgccg ccgaggaggg gagcgaggtc tggcggcccg cccgggtcgt
     2341 cgacgtttcg ctgggcgggg aagcgggaag ctcggtcacg gtggagaagg ccgacgggac
     2401 gcgcgtgacg gtccgcgcga agtgggtcgt cgacgcctcc gggcgccagg cgctcctcgc
     2461 gcgcaagcgg ggcggcatcc ggccggtcga gggccacccg acctcttcga tctgggtccg
     2521 ctatcgcggc gtcaaggacc tcgacggcaa ggaggtcgtc ggtaccgacc cggccgatcc
     2581 cttcgtccga agcgtcgtcg cctcccgaag gctcgccacg aaccacttca ccggctgggg
     2641 ctactgggtc tggttcatcc ctctccgcgg tggcgagacg agcgtcgggc tcgtctggga
     2701 cacgcggctc gtccgccccg aggggtcgac accgctcgag aagctgacga gcgtcctctc
     2761 cgcgaacccg ctgacgcgcg agatgctcga gaacgccgag ccggtcgagg gggactgccg
     2821 ctactacggc acgctcccgt acttcctcga ccgcttcatc gaccacggct ggacctgtgt
     2881 cggcgacgcg ggcggcttcc tcgacccgtt ctactcgccc gggctcgacc agatgagctt
     2941 ctcggtccac atgcgggtcg agctgatcct caaggcgctc gcgggggccc cgcgcgagga
     3001 gatggcgaag gagtacgagg accacaaccg aaagtacgcg cggttcttcg agtacttcta
     3061 cgaggcgatc taccgcgaca agtaccacct gatgggcgat tacgacacga tgacgacggc
     3121 gttcctgatg gacacggctc tctactacct cgtcgcggtc cagcccgtct acaccgaatc
     3181 ggccggccgc ctcgggatcc cgccgtacta cggcgacggc gccgaggtcg gcctcgtccc
     3241 gatgcgcttc taccagcagc gcctcgtctc gatcgcgaaa cggaagaagg cgctcgggat
     3301 ctacgggaac cacaacgccg ggcgccgccc cggcttcgtc gggttctcgc tccggagctc
     3361 cgcccacgtg atgctgtggc acggactcct ccgctggtgg aaggccgagc tcgcgaacgc
     3421 gctgacgtac gtctggaagc cgcggccgct cgaagggccg cagccggtcc tcccgccccg
     3481 gccggcggag gaggccggcg ccgaggcgcc ggcgcgaggc tgacgggcgt gccgggcgcg
     3541 cgttccgggc gcctcctcgc gccgctcgcg gcgggcgcga tcctcctcgc gggggccctt
     3601 ccggcgcgcg cctgggacgt cgacgcgctc gtcctcgagg cggccgcccg gcagcgcgag
     3661 ggagaggtgg ccgcggggcg gatcgccctc gagcgcgtcg aggtggagac ggagctcgac
     3721 gcggggggac ggaggaagac gtccgagcgg agacggtacg tcgtcgtgat ggaccggcgc
     3781 ggcctcgccg cccgggagct cctcgaggtc gacgaccgcg cggcgacgtc ggcggagagg
     3841 gaggaggcgc ggcgtcagga cgagaagcgg aaacgcgccg cgctcgagcc ggggagcgcg
     3901 cgttccgacg aggaggagct cctgtcgggc cgcgtcccgc tcctcgacct ctaccatcgc
     3961 ctcgactttc ggcttctggg cgaggagcgg atcgacggcc ggccgacttt cgtcgtcgcg
     4021 ttccggccga agcccgggct cgtcgcgagg acgatccggg accgcgtcct gaacgccttc
     4081 gcggggacgg cctggatcga cgtcgcggag atccaggtcc ggaagatcga cgggcggctg
     4141 acgcggcccg tccgggtcct cggtggggcg ctcctcgagg tcaagggcgt gcgtctcctc
     4201 tacgagacgc gcgaggtcct tcccggcctc tgggccccct gccgcgagga gatcctcatc
     4261 acggcgacgg ccgcgatggt cctcccgttc cgtcgcgagc tccgcttcga gttctcgagc
     4321 taccgccccg ccacctgagc cgccccccct gagccagggg tctggtctac actctacgct
     4381 ctgctgtcga gcagagtgta gagtgtagac cagacccctc ctcctaatcc tcgagcgtcg
     4441 accctcccgg gtttcagctc cggacgaagt gggggaggtc gaacgggatc ggaagcgaga
     4501 gctcgacgat tccccggcag acgccgttct cgtaccaggg ggcctggtag atgagcttct
     4561 tcaccccgtt cttctcgatc gtgtaggcgt tcgttccgcc cgtgcggagg agcacctcga
     4621 agagctctct cgcgcgcggc ggatggcagt cgaggatgct cttcccgacg aggtcgcgcc
     4681 cgccgtccgc ggcaaacgtc tcggccgccc ggtcgttcat ctcgagggcg gtcccgtcgc
     4741 gatcgcagac cgtgatcccg accttcagct ccttcaccca ggctccgtcg cccatggcgt
     4801 ctcctctctc ctcgcgcccc gccgtcctcc gcgacgcgcg aggagagatg gtacggaatc
     4861 gcgaggcgtc cttcagccct gggggatcgg gccgatgctc gggttcggct cgaagctcag
     4921 gagcatccgc tcgaggacct cggccgcctc gtcgcggcac ggcttcggcg cggccgtcag
     4981 gccgccgagg accgaatacg cgccggtcga gttcccggcg atcgggtaga gcgcgacgaa
     5041 gcccgacatc tcgcccatct tcgtccgctg gacgtacatc gtccggccgc cgacgctctt
     5101 ctcgaggacc ttcacggtcg ccggcagctt tcgccagacc gcgacgagct tctccgggcc
     5161 ggccaccccc ttcgtggggt aggcgaaacg gaggacgtac tcgtcggccc gatctccgtc
     5221 gaggtcccgg gtcacgtcga cgtgcaccgt ctcccccttc gagaagtcct tcgggacgaa
     5281 tccctccggc atctcgaacg agaaggcgag cggggacccg gccgcgtcgt gccgcgtcat
     5341 cggcgcgcga aggaccgcgc agggatccga ggcggcctcg gaccgtgccg ccggcgcggc
     5401 cccttccccc acctggggcg cgggggcggg cgcctcggga gcttccggct tcttcgcctg
     5461 cggaccacag gcggcggcga aggccgcgac gacgaggacg acacgggcag ctctcatgga
     5521 agacctcctg ggttcgtgat gccggtacct tagcagcacg atgggggtgt accgcccccg
     5581 ctcagcgtcc ggggagcttt cgcccctcct cgagccagcc gcggagatcc gcgagggagc
     5641 gttcccagct cggtccgagc ccggcggcgt agccgtccca cgaggctccc tcgccgacac
     5701 cctcgtgccg cagcgagacg cgcgtcgcgc cgttctccga ggcgatctcg atcgagagtc
     5761 gcatcccctc gagcagctcg ccctccgggg tggagaagcg gaggtcccgc acgacgaggc
     5821 ggctcggcgg ctcgaaggcc tcgaccgtgc cgaggacgtg gtagccgtcc tcggagtcct
     5881 cgtcggcgta ccagccgagg gcccagttcc cgccgacgcg cggaccgatg accgccgcgt
     5941 cggcgagcca cgcgaggacc tccgggacgt cggtgaaggc cgtgaagacg cgatcgggag
     6001 acgcgttcag cacgatgtcg cgggagacgg acctcatcgg cacgggatca tagaggctcc
     6061 gctccgctcg acggctggtc tcgaggaagc gcgcccgtca cgacgagctc gaccctgccg
     6121 acgagctgcc cgtgcgccgt ctccacgtcg aggcggagac gctgcccggg cgcgagccgg
     6181 ccgaccgtca cccccgaacg agagcggaac ccttcccgcc ggccgccggt gatcccgatg
     6241 acgcgagacc ggaagaagac ccggccgtcg agcgcccaga cgtggcggac cccttcctcg
     6301 aggccggccg gagcgaagac cgcggcgacg gcgaagagcc gccggccggg ccctccgcgg
     6361 agcgacgact gcggagaagc gacgtcgaga ccgttcgggg cgagcgcgcg gccgacgcgg
     6421 gcgctcacga gccgcagcgg ggcgggcggg acggcggggc gaccgagtgt gacgagggcg
     6481 aggaagaggg ctccgacgag cgccgcgcgt ccgagccttc ggtcccttcc ggacgaggcg
     6541 acgaaggaga cgaagacggc cgtcgcgagg aggccgctcg cgccgaggct ccagaggccg
     6601 cccgtgcccc agaccatcgg cagcgtgacg ttcacggtcg cgaacgcgag gaacgcgagg
     6661 agtgccccgt gcagggggcg ccggggcgcc acgacgtcgt cgtagacgac gtcgatcgtc
     6721 gccgcgaggg cggcggcggc gaggagcgcc atgaaggccg cctggcccgg cgtgtaggcc
     6781 gcgcttcggt gatagaccgg caggaggaag aacgcgagcc cctgcaggag ccacttctgg
     6841 gcccagagga cgagccggcg cgccggctcc tttcgctccg cggcgagcca gccggcggag
     6901 aggagcgcgg ggagcgcgag gctgctcccc cagacgccgc cgatcgagac gagcccccag
     6961 cgcagcagct ccggccgctc ccgccccgcg atcacgaagg cgacgcccca gccgagggcc
     7021 cagagggaat gcagcgtcca gaagacggcc tgatggcgcg ccgcgaaccg ggcgaacctc
     7081 ccgagcctct cttcccagcg ccggcgcagc gggccgcctc cctctcccgc ggcgcaccgc
     7141 gggcgcgaga ggatacctgc ctcccgcgga cgagggcgga aaggacgtct cccccctgcg
     7201 gtcaggcccc gcggctcccg cgcagcgtct cgagagccac gagccgctcg atggcgtcga
     7261 gctcgaggtc gtccttgccg agctcccgca gccgcgccca ctcgcggcgc agctcccggg
     7321 cgagcgcttc gggaagcgtc cggagccacc cctcgatacg gaggtcgacg aactccctct
     7381 tctcctcgag caggagctcg cgggcgagga ggaggcgcgt cgcgtcctcg gcgtcgacga
     7441 gcccctggag gaccggccag cgttcgcccg gcctctcggc gcagagcgcg gagaaggcgg
     7501 cgctcgaggt ctcgcggatc agctcctcct cgcgcgtcgt ccggctctcg tgctccttcg
     7561 tctcgtccca gctctccgag tcctccgcga gccaccggag gtactccatc gcgaagaggc
     7621 aggtcggcat cacgaggtcc tcgcgcgtcc gcgcgtcgac ccagagcgcc gccgcggccc
     7681 gcaggcaccg gcgcgcggtc gtctcggcga gcttccggcc gaagacgagc ccggccgcct
     7741 cccgcacgaa gaacggcagc tcgctcgcga cgatctcgcg gtagccgggg acgtgccgct
     7801 cgaggaggac ggc
//
LOCUS       DAOONL010000144         7666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11265_length_7666_cov_12.747865, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000144 DAOONL010000000
VERSION     DAOONL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11265_length_7666_cov_12.747865"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2116)
                     /locus_tag="PLP50_14885"
     CDS             complement(<1..2116)
                     /locus_tag="PLP50_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HPA52878.1"
                     /translation="MAIVAEKPSVGRDLAAVVGATRRGDGCLVGDGWIVTWAVGHLVT
                     LAEPGQMDPAWQTWRRDRLPMLPSAWPLVVLDRAAEQFAAVRRILSDPEVVEVVCATD
                     AGREGELIFRQLYEAARCTKPVKRLWISSLTPEAIRAGLAGLKPARDYDRLADAARGR
                     SRADWLVGMNFSRAFTLAHGEALSVGRVQTPTLAMLVERELAIRAFVPEEYFEVIATF
                     EGETGCYRGTWFDPTVKEEGGRRLPKDGAKAKEIVARVREGMAAVASAESKTRRVPPP
                     LLFDLTELQRHANRLLGWSAKKTLDVAQRLYEEKKAITYPRTDSRHLSRDVAATLPRL
                     AAALEPSWGRLFAPGTGERPLGARVVDDAKVSDHHAIVPTPNVPSGLKGEEKALYEIV
                     VRRLLAAWHDDHVAATTTVVTEVTGGEGDRGAATGAPAGRPVDRFRSAGTMVVAEGWK
                     AAEPDAPRRKKDEDDEEGELPPGLAKGQAKRVVDAAAVEKRTRPPKRFTDATLLTAME
                     TAGRTLDEKELAEAMKENGLGTPATRAEILETLIARGYAVRDGKVFSVTEKGIRLIGL
                     VPDSVKSPALTGKWEAELARMQQGDGALPTFMNGIEAYVKEVVAGTFRAAPSPAAPEM
                     PEAGTRPETGSPPMGSGVKSCPAGAIHDFTPDPRPCKPRVAGPVPAERLGELLEEVFG
                     HEAFRPYQEAVCRAVVEGRDALL"
     gene            complement(2285..4207)
                     /locus_tag="PLP50_14890"
     CDS             complement(2285..4207)
                     /locus_tag="PLP50_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPA52879.1"
                     /translation="MDEPATDVRESTDGAQEAAAPAVVASLVSDLRAAKAELEARNEA
                     LRLVNDLAFRLQRRLDPEAIAVETAEALRGGSAPPLVAIYLLDGPDGPLRLVAGRGFT
                     EEELDLGARLPLEGSASGRAIRERRIVSLGEVRIEGREVHPVVDALARRRVASGLCVP
                     LAHGGRSLGTLNVLYPEGRDLRPEEAETFEGVARAVSLAVANAQYRTELEERAYRDAL
                     TGLPNRAGFHRRFGTLAEAVGGGRAALVLLDLDWFREINDTLGHNFGDELLVQVGTRL
                     AERCSGGMEVFRLGGDEFAIVVPAAERREDGEDAARKVLAWLGEPFRVAGMGLEVGAS
                     AGVAHFPTDARVSHGLLRCADVALHVAKKGAGGVAGYEADLDEHTPERLTILSDLGRA
                     IREGQLVLHFQPKVALQYDFIEGFEALVRWRHPRLGLLGSSQFVPFAEATDGMVALTS
                     WVVRRALEQLSLWNRQLPKLTMAVNLSARNLVDRSCPEKLEEIVRSVGVDPGLVEFEL
                     TESAVMSDPETALKMLGRMHDSGARLAIDDFGTGYSSLAYLTRLPVDVLKIDRSFVAG
                     MATGGKDLAVVRSTVQLAHSLDLGVVAEGVEDAAAARTLREMGCDLGQGYFFAPPAPA
                     EEAGRLLTGGGFLTGR"
     gene            complement(4417..4848)
                     /locus_tag="PLP50_14895"
     CDS             complement(4417..4848)
                     /locus_tag="PLP50_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52880.1"
                     /translation="MKQNHFRLFAILIGLLVCAGAGYGVFHFSQVPADGHFVDPAFAP
                     PLAESAGRVVVYVAGEPILKAIEAQKVVLKRKDGEAPLAASEIVASLNERDRVRLTKV
                     PLMLGLAALSGGAFVLFLIGLFTPLISALKPPMIVDLHLEE"
     gene            complement(5093..5842)
                     /locus_tag="PLP50_14900"
     CDS             complement(5093..5842)
                     /locus_tag="PLP50_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52881.1"
                     /translation="MTRRSALLALLFLALGALTGRPALAEGFRLDLETGAAWQLRNDL
                     AVPGEGGTRLSIDDADRGPFFTARATLWAELGERSSLRVVAAPFRTKATLVPQTAVAF
                     DGSTFAAGEPLRATWVFDSWRLSYVWRFRSSGPVSFRAGLTAKVRDARIELEQGGVSQ
                     AKTNTGFVPLLYGGVRWQVTPGLAVDLEADGAAASQGRAVDASVRLEAKVSPGVDLFL
                     GARTLEGGVDGDEVYSFAWLAYGVGGVSLRF"
     gene            complement(5895..6734)
                     /locus_tag="PLP50_14905"
     CDS             complement(5895..6734)
                     /locus_tag="PLP50_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52882.1"
                     /translation="MRRGGEGALLVLIVALLAAPGCGLPERVRRLVSRPTPTPLPRPT
                     PVPAPPLELSFLGIHEDRVRGGAAGPAGTTLEVALVGTKRGDVETARVVVATAVDDLG
                     ASLVPAGAANAAHEPPRGDDATAPVVLSVPLKLASRSAATLREVSGSIELYVPDADPD
                     AAVVVPRFRSLAGSPLVSPSLARNDVTIAIAATQEAEALPADPDELVLRVADPLGRVE
                     GFYFVDPEGTAWATDREERGGLVVVSARSEAPGPDWGLRVRLKTPSTLRLYRFVLKDV
                     PLP"
     gene            complement(6731..7495)
                     /locus_tag="PLP50_14910"
     CDS             complement(6731..7495)
                     /locus_tag="PLP50_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52883.1"
                     /translation="MKRQDAVFAASLVAVALAPAVADARSADGGFRLVVEAVAESRQR
                     SASGETEGRLTLQPKLEGDGLASAKAFRIRVSAAADATGRDVLPEEPEPSRWEVRPSG
                     TGLWIVLGSPARGAAFVDVSGTIDLWIPSRDAGAEARIPRALARPGAPLAASTLRSEG
                     VTLRVAPRVADAPTVVSLRGSAADVERVRTVRVLRPDGTEISASSLQVTIAEGKGSLE
                     LILAEPPPADATLVLGLLTRRSVVAVPFELKGVALP"
BASE COUNT         1056 a         2889 c         2716 g         1005 t
ORIGIN      
        1 cgaggagcgc gtcgcgcccc tccacgaccg cccggcagac cgcctcctgg tacgggcgga
       61 acgcctcgtg cccgaagact tcctcgagga gctcgccgag gcgctccgcc ggcacggggc
      121 ccgcaacccg gggtttgcag ggtctggggt caggcgtgaa atcgtgtatc gctccggcag
      181 gacacgattt cacgcctgat cccatcgggg gtgaccccgt ctcggggcgt gtccccgcct
      241 ccggcatctc cggcgctgcg gggctcggcg cggcgcggaa ggtgccggcg acgacctcct
      301 tcacgtaggc ctcgatcccg ttcatgaagg tggggagggc gccgtcgccc tgctgcatgc
      361 gggcgagctc ggcttcccac ttcccggtca gggcggggct cttcaccgag tccgggacga
      421 ggccgatgag ccggatccct ttctccgtca ccgagaagac cttgccgtcg cgcacggcgt
      481 agccgcgcgc gatgagcgtc tcgaggatct cagcccgcgt ggcaggcgta ccgaggccgt
      541 tctccttcat cgcctcggcg agctccttct cgtcgagcgt ccggccggcc gtctccatcg
      601 ccgtcaggag ggtggcgtcg gtgaagcgct tcggcgggcg ggtcctcttc tcgaccgccg
      661 ccgcgtcgac gaccctcttc gcctgcccct tcgcgaggcc cggcggaagc tccccctcct
      721 cgtcgtcctc gtccttcttc cggcgcggcg cgtcgggctc ggcggccttc catccctccg
      781 cgacgaccat cgtcccggcg gagcggaagc ggtcgacggg ccttcccgcg ggcgctccgg
      841 tggccgcgcc ccggtccccc tctccgcccg tcacctccgt cacgaccgtc gtcgtcgcgg
      901 cgacgtgatc gtcgtgccag gccgcgagga ggcgccggac gacgatctcg tagagcgcct
      961 tctcttcgcc cttcaggccg ctcgggacgt tcggcgtcgg gacgatcgcg tgatggtcgc
     1021 tcaccttcgc gtcgtcgacg acgcgcgcgc cgagcggccg ctcgcccgtc cctggcgcga
     1081 agaggcggcc ccaggagggc tcgagcgccg cggcgagccg cgggagcgtc gccgccacgt
     1141 cgcgcgagag atgccggctg tcggtccgcg ggtaggtgat cgctttcttc tcctcgtaga
     1201 ggcgctgggc cacgtccagg gtcttcttcg cgctccagcc gaggagccgg ttcgcgtggc
     1261 gctgcagctc ggtcaggtcg aagaggagcg gcggcggcac gcggcgcgtc ttcgactcag
     1321 ccgacgcgac ggcggccatt ccctcccgca cgcgcgcgac gatctccttc gctttcgccc
     1381 cgtccttcgg gaggcggcgc ccgccctcct ccttcaccgt cgggtcgaac caggtcccgc
     1441 ggtagcaccc cgtctcccct tcgaacgtcg cgatgacctc gaagtactcc tcggggacga
     1501 acgcccggat ggcgagctca cgctcgacga gcatcgcgag cgtgggcgtc tggacccgtc
     1561 ccaccgagag cgcctccccg tgcgcgagcg tgaacgcgcg cgagaagttc atcccgacga
     1621 gccagtcggc ccggctccgt ccgcgcgccg cgtcggcgag ccggtcgtag tcccgcgccg
     1681 gcttcagccc cgcgagcccc gcacggatcg cctccggcgt cagcgacgag atccagaggc
     1741 gcttcaccgg cttcgtgcag cgcgccgcct cgtagagctg gcggaagatc agctctccct
     1801 cgcgccccgc gtccgtggcg cagacgacct ccacgacctc ggggtccgac aggattcgcc
     1861 gcacggccgc gaactgctcc gcggcgcggt cgaggacgac gagcggccac gccgagggga
     1921 gcatcgggag gcggtcgcgt ctccaggtct gccatgcggg gtccatctgt cccggctcgg
     1981 cgagggtgac gaggtggccg acggcccagg tcacgatcca gccgtcgccg acgaggcagc
     2041 cgtctccgcg ccgggtcgcc ccgacgacgg ccgcgaggtc cctcccgacg ctcggcttct
     2101 ccgcgacgat cgccacggtc cgaccgtgtc gtccgcggac gtcccctccc gtcggtcccg
     2161 cctccccctc gcgccgggcc ctcgctcgct cgaccaacgt ggtcctccct cgacccccca
     2221 ccggggccgc cggaggacga tagagcatcc ggacggcgca accgcgcgcc cggggccggg
     2281 cgcgtcaccg tcccgtgagg aagccgccgc ccgtgagcag gcggcccgcc tcttcggccg
     2341 gtgccggagg cgcgaagaag tagccctgcc cgaggtcgca gcccatctcg cgcagagtcc
     2401 gcgccgcggc ggcgtcctcc accccttcgg cgacgacccc gaggtcgagg ctgtgggcca
     2461 gctggaccgt cgagcggacg acggcgaggt ccttcccgcc cgtcgccatc ccggccacga
     2521 acgaacggtc gatcttcagg acgtccacgg ggagccgcgt gaggtaggcg agcgaggagt
     2581 accccgttcc gaagtcgtcg atcgcgagac gggcccccga gtcgtgcatc cgcccgagca
     2641 tcttcagcgc cgtctccggg tccgacatca ccgccgactc cgtcagctcg aactccacga
     2701 gccccgggtc gacgccgacc gacctcacga tctcctccag cttctccggg cagctcctgt
     2761 cgacgaggtt cctcgcggac aggttcacgg ccatcgtcag cttcggcagc tgccggttcc
     2821 acagggagag ctgctcgagc gcccgccgga cgacccacga ggtgagcgcg accatcccgt
     2881 cggtcgcctc ggcgaagggc acgaactggc tcgagccgag gagcccgagc cgggggtgcc
     2941 gccatctcac gagcgcctcg aacccctcga tgaagtcgta ctggagcgcc accttcggct
     3001 ggaagtggag gacgagctgc ccttcccgga tcgcacgccc gaggtcggag aggatcgtca
     3061 ggcgctcggg cgtgtgctcg tcgaggtcgg cctcgtagcc ggcgacacct ccggcgccct
     3121 tcttcgcgac gtgcagcgcc acgtcggcgc agcgcaggag cccgtggctc acccgcgcgt
     3181 cggtgggaaa gtgcgcgacg ccggcgctcg cccccacctc gagccccatc cccgcgaccc
     3241 ggaacggctc cccgagccag gcgaggacct tccgcgcggc atcctccccg tcctcccggc
     3301 gctccgccgc cggcacgacg atcgcgaact cgtcaccccc gagacggaag acctccatgc
     3361 cgccgctgca ccgctccgcg agccgggtac cgacctggac gaggagctcg tcgccgaagt
     3421 tgtgcccgag cgtgtcgttg atctcccgga accagtcgag gtcgaggagg acgagggccg
     3481 cccggccacc cccgacggct tccgcgagag tcccgaagcg cctgtggaag ccggcgcggt
     3541 tcgggagacc cgtgagcgcg tcccgatagg cgcgctcctc gagctccgtc cggtattgcg
     3601 cgttcgcgac ggcgagcgag accgcgcggg cgaccccctc gaaggtctcg gcctcctccg
     3661 gccgcaggtc ccggccctcc ggatagagga cgttcagcgt tccgagagac cgccccccgt
     3721 gcgcgagcgg gacgcagagc cccgaggcga cccggcggcg ggcgagcgcg tccacgaccg
     3781 gatgcacctc gcgcccctcg atgcgcacct ctccgagcga gacgatccgc cgttcacgga
     3841 tggccctccc gctggcgctc ccctccagcg ggaggcgggc gccgaggtcg agctcctcct
     3901 ccgtgaagcc gcgtcccgcc acgagccgaa gtggcccgtc gggcccgtcg agaaggtaga
     3961 tcgccacgag cgggggcgcg ctccctccgc gcaacgcctc cgcggtctcc acggcgatcg
     4021 cctccgggtc gaggcgccgc tggagacgga aggccaggtc gttcacgagt cgcagggctt
     4081 cgttgcgggc ctcgagctcg gccttcgccg cccgcaggtc gctcacgagg gacgcgacga
     4141 ccgcgggcgc ggccgcctcc tgagctccgt ccgtcgattc gcgaacgtcg gtggccggtt
     4201 cgtccatcgc gccctctccc gcgcgccggg cgggtctccc ccaccgcgcg gccccgtgtc
     4261 tgacacggct cgccgtcgac gaatcgatca tatcccgcag cgacggcacc accggcctcc
     4321 gcaggggggc tcgcgcggtc cggagacgtg gcggagagcg ggacgggcgg gcggagtccg
     4381 ggcgcgtccc cgaccggaat cgcgcccgcg ccggcgtcac tcctccaggt ggaggtccac
     4441 gatcatcggc ggcttcaggg cgctgatgag cggcgtgaag agcccgatga ggaagaggac
     4501 gaacgcgccc ccggagagcg ccgcgaggcc cagcatcagg gggaccttcg tgagccgaac
     4561 acggtcgcgc tcgttgaggc tcgccacgat ctcggaagcg gcgagcggcg cttccccgtc
     4621 cttccgcttc aggacgacct tctgcgcctc gatcgccttc aggatcggct cgcccgcgac
     4681 gtagaccacg actcggccgg ccgactcggc cagcggcggt gcgaacgccg gatcgacgaa
     4741 atggccgtcg gccgggacct gagagaagtg aaagacgcca tatccggcgc ccgcgcagac
     4801 gagcagaccg atcagaatcg cgaacaggcg aaagtggttc tgtttcaccg tcttgcctcc
     4861 ccgggagtcc ggctcggcgg taggaggaag gcccgctccc ctccggaggc cgcggcgagc
     4921 cggcgagtcc gggaaccggg ggagaggcgg aggcgtcgga ggaaggggcg gccggttccg
     4981 gcgccgttcc gatgatagcc cttcgcgcca ttcccgggcc gcctcgtcct tcacgcgacg
     5041 agcgaggtgc cggtcgcccg tcgcagctcg tcacccggga cggaacgccg tctcagaaac
     5101 ggagagagac gcctccgact ccgtacgcga gccaggcgaa gctgtagacc tcgtctccgt
     5161 cgacgcctcc ctcgagcgtc ctcgcgccga ggaagaggtc cacgccgggc gagaccttcg
     5221 cttcgagccg gacggacgcg tcgaccgccc tcccctggct cgcggcggcg ccgtcggctt
     5281 cgaggtcgac ggcgaggccc ggcgtgacct gccagcgaac gccgccgtag aggagcggga
     5341 cgaatcccgt gttcgtcttc gcctgcgaga cgccgccctg ctccagctcg atccgggcgt
     5401 cacggacctt cgccgtcagc cccgcgcgaa aagagaccgg ccccgaggag cggaatcgcc
     5461 agacgtacga gaggcgccag gaatcgaaga cccaggtggc ccgaagcggc tctcccgcgg
     5521 cgaaggtgct tccgtcgaag gcgaccgcgg tctgcggtac gagggtcgcc ttcgtccgga
     5581 acggggcggc gacgacgcgg agggagctcc tctctccgag ttcggcccac agcgtcgccc
     5641 gggcggtgaa gaagggaccg cgatcggcgt cgtcgatcga gaggcgcgtc ccgccctcgc
     5701 cgggaacggc gaggtcgttc cggagctgcc aggcggctcc cgtctcgagg tcgaggcgga
     5761 acccttccgc cagcgcaggc cgtccggtca gggccccgag cgcgaggaag aggagagcga
     5821 ggagcgcgga gcgacgcgtc acgctcccac tccgagagcg cgctcccgcc ggattcctcc
     5881 gctcgccccc ggggctacgg caggggaacg tccttcagca cgaaccgata gaggcggagc
     5941 gtgctcggag tcttcaggcg gacgcggagc ccccagtccg gaccgggtgc ctcggagcgc
     6001 gccgagacca cgacgagccc gcccctctcc tcgcggtccg tcgcccaggc cgttccctcg
     6061 gggtcgacga agtagaagcc ctcgacgcgc cccagcgggt cggcgaccct gaggacgagc
     6121 tcgtccgggt ccgcaggcag agcctccgcc tcctgcgttg ccgcgatcgc gatcgtgacg
     6181 tcgttccggg cgagcgacgg agacacgagg ggtgacccgg cgagggagcg gaagcgggga
     6241 acgacgacgg cggcgtccgg atcggcgtcg gggacgtaga gctcgatcga acccgagacc
     6301 tcgcggagcg tcgccgcaga gcgggaggcg agcttcaacg gaacggagag gacgaccggc
     6361 gccgtcgcgt cgtccccccg cgggggctcg tgggcggcgt tcgcggcgcc ggccgggacg
     6421 aggctcgcgc cgaggtcgtc gacggcggtc gcgacgacga cgcgcgccgt ctcgacgtcg
     6481 cccctcttcg tcccgacgag cgcgacctcc agcgtcgtcc cggccgggcc cgccgccccg
     6541 ccccggaccc gatcctcgtg gatgccgagg aagctcagct cgagcggtgg cgcgggaacc
     6601 ggagtcggcc gagggagcgg cgtcggagtc gggcgcgaga cgagccggcg cacccgctcg
     6661 ggcaggccgc accccggagc cgcgaggagc gcgacgatca ggacgaggag cgcaccttct
     6721 ccgcctcgtc tcacggcagg gcgactccct tcagctcgaa cgggaccgcg acgaccgatc
     6781 gcctcgtcag gaggccgagg acgagcgtcg cgtcggcggg cggcggctcc gcgaggatca
     6841 gctcgagaga gcctttcccc tccgcgatcg tcacctggag gctcgaggcc gagatctcgg
     6901 ttccgtcggg gcgaaggacg cggaccgtgc ggacccgttc gacgtcggcg gcgctcccgc
     6961 gcagcgacac gaccgtgggc gcgtccgcga cacgcggcgc gacccggagc gtgacgccct
     7021 cggatcgcag ggtggacgcc gccagcggcg cacccgggcg ggcgagggcc cgagggatcc
     7081 tcgcttcggc cccggcgtcc cgcgagggga tccagaggtc gatcgtcccg gagacgtcga
     7141 cgaaggccgc gccgcgcgcc gggctcccca ggacgatcca gaggcccgtc ccgctcggac
     7201 gcacctccca tctggagggc tcgggctcct cggggaggac gtcgcgccct gtcgcgtcgg
     7261 cggcggccga cacgcgaatg cggaacgcct tcgcgctcgc gagaccgtcg ccctcgagct
     7321 tcggctggag agtgagccgc ccttcggtct cgcccgacgc ggagcgttgc cgcgattcgg
     7381 ccaccgcttc gaccaccagc cggaatcccc cgtcggcgga gcgcgcgtcc gcgaccgcgg
     7441 gggccagcgc caccgccacg agagaagccg cgaagactgc gtcctgtcgc ttcataccga
     7501 gctcgggccg aacagctccg gaagtgtacg tcgaacgctg cttcgatcgc gcggtgctcg
     7561 ccgccccccc cgggaggcca ccgtccggtc cgcccgaccg agtcggacgc ctcccgagaa
     7621 tcgaggaagg gtggggacgc gatcgcgaca ggctcagaag atggcg
//
LOCUS       DAOONL010000145         7655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11287_length_7655_cov_9.187368, whole genome shotgun sequence.
ACCESSION   DAOONL010000145 DAOONL010000000
VERSION     DAOONL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_7655_cov_9.187368"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLP50_14915"
     CDS             <1..321
                     /locus_tag="PLP50_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52884.1"
                     /translation="PGTADWTNLRSLVGLALPRVEERLDSAASPLLLTWPGLLVRYER
                     LDLLDRLRDRHTRAGASGAAWLLIAADEQSELPVLDGRPLPVLSPAQYARVPHAWLEA
                     TEGM"
     gene            complement(340..5898)
                     /locus_tag="PLP50_14920"
     CDS             complement(340..5898)
                     /locus_tag="PLP50_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPA52885.1"
                     /translation="MTGPALLSDFLRVRIEEGPLPTDDALALVLPLLGQVEALHERGL
                     VSPLEGLAEVRVDGGRAWFPEALARPIRRNAKVGELGARESEALDVVGHAKVVTDVDQ
                     GWSEVESLSVAAGTGPVERRCYVPGFRSWEHTLGHHDPLTDTFVLGLILGSVLLGLDL
                     GEEGDLRTFASARENLFRLNPGLNPVVAKAIVRMTELDRHRRPQDLGRLGKTLENYRD
                     QEVDFDFDLARVAGFKERDPKGRRQVILSRLQERLFEVTRRNRLLHFRSTLQSVNLTQ
                     ASVPMVLDVRSIRPDQLLTWGPVLRRLVVDGDPIPLGRYLRFEDHVFLPGVLDQIRAE
                     ARRDRAEYGFSQLRLVLCMLRWTDLKETRERFESPLVLIPVELEKKKGVRDVHLLRPL
                     TAEAEVNPVVRHVFRQSYGIDLPESLDLAETDLDAFFDFLVKRVQASEPGVSLEKIEH
                     PRIELIHDRARRRLDQYRRRARSGARSARSFQSLDYSYGPESFAPLGLKLFEARVRAP
                     ETRLRSLLDETPRTRSFAVPELTPGAVAERDSLHYHLKTDAAGNPYAWEFDLCSLTLT
                     NLRSRKMSLVRDYEGLLSQDVPSDSFDRVFSLAPRAPEEALPEEPFEARFPVVPCDPT
                     QSSSIAAVANGRSLIVQGPPGTGKSQTITNLIAELVARGKRVLFVCEKRAAIDVVHHR
                     LKQSGLEEIVCLVHDSQQDKKAVIQDLKATYEGWLARTSDEPGRREREREDLVRSIRR
                     DLAPIEQYDRAVLGVPEPAGIAVQGLIERLLEIGPSAPPLDPIEAERLPAYRAWVENE
                     GALRRLQSLVEEIEPTGVLARHPLRRLSPALADEARPVERVSRAVAEARTLLAEITDR
                     LEASDLPRRGWDGLAALLALEAYSRMATPFLERGALELLEPGSTLSKEAAKLLAEQEA
                     LVANVARRKDATVGWRSKLSPNDTEAALGQAREVQGALGFLKPAWWRLRRLLGERYDF
                     RSSAVRPTRVQVLEWLLEEHRAEAAAREGEERIESRFGLGKPEELRRRLDEARAELAA
                     GSRPIRELHERLLSGSGGGLAILGELGAAGRSAMDLRDALHGVVERPESLGLDELRAQ
                     LEGIREALSQVSEFLSCLAALAALPAGLADSFRDLPCEARGVEAALAWKAFREALRSD
                     RVLGRFSSLERSLGVRRVEEAYRRWLAANAGTVLERARGRFFENVRLSSLPDAGLPAD
                     QKARKRAYVRGRKELERELGKSMRFRSIRELLQSEAGPVILDLKPVWLMSPLSVSDAL
                     PLDSRQFDVTVFDEASQIRLEDAVPCLFRSGQVVVVGDQMQLPPTSFFSAANTEEESF
                     TVEENGEVVEYDLDSDSLLHHSEKSLPATMLGWHYRSRSESLISFSNAAFYGNGLLTV
                     PERVVAGRPMDEICPHPGTGAGIDTDAILARPVSFHHLAEGVYSDRRNEGEARHVAEL
                     VRALLLRGDRPTIAVVAFSEAQQGEIEGALQELARTDEDFARRYEAELEREDDDQFVG
                     LLVKNLENIQGDERDVVILSVCYGRDPQGKVRMNFGPINQSGGERRLNVAFSRAKRHM
                     AVVSSMRGADITNDFNDGAACLKRYLRYAEATSKGDAEGARRVLDELAPARRKESFQA
                     ETAAVQLAESLRDRGYEVDLDVGTSRFRCPLAIRRPGEAEYRLAVFLDTRRHFAETER
                     LGRDVLKPALLRSFGWRSEFVFAREWLLEPDAVLLRLEARLAGVEPEVAPAQSSAPEP
                     EPESAPELELEPEPEPEPGREPGPDPDPVSGSPADEEPELKKTLGVELDGFSFQRYLE
                     FVGGGSSKFWEIRVRDCESFVRFGRIGTAGQERVARHATSTAAETEARKLADSKARKG
                     YVVKERPGSA"
     gene            complement(5895..7121)
                     /locus_tag="PLP50_14925"
     CDS             complement(5895..7121)
                     /locus_tag="PLP50_14925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPA52886.1"
                     /translation="MIAEVAAPRLTPLAYQRAIVEYLRSEEPGIWEWFSSNRFRAKHA
                     EQVRLDILKSTYRLEREGHAELYAIADEVESALGLEIPLTIYQAPAAEGGMNAFLAWI
                     PGEAHVVLKGPVLSSLRPGELRALLGHELSHAILYALEEGAFLVASQVLEAMAGDASA
                     DPSHVESARLYDLYTEVFADRGSLLASGNAADSIAALLKVETGLAEVSAESYLRQADE
                     IFAQGSPRAEQLTHPEAFIRARALALWESKGEKADSEVACMLEGNPALGALDLVARRR
                     VEGLTRDVVAAYLGPVPFRTESVVGHARRFFPDFSPSARSQGDLDRLRGFVETCDASI
                     RDYFCYVVLDLVAVERELEDVPLARGLVLAAELGLEARFSEVAAKELGLSKKALAAAR
                     AGAAGLVSRVVAEASA"
BASE COUNT         1140 a         2771 c         2605 g         1139 t
ORIGIN      
        1 cccggcaccg ccgactggac gaacctccgc tccctcgtcg gccttgccct gccgagagtc
       61 gaagagcgcc tcgactccgc cgcgagccct ctcctcctca cctggccggg cctcctcgtc
      121 cgctacgagc ggctcgacct cctcgaccgc ctccgcgacc ggcacacccg cgcgggagcc
      181 tcgggcgccg cctggctcct catcgccgca gacgagcagt ccgagctccc cgtcctcgac
      241 ggcaggcctc tccccgtcct cagccccgcg caatacgccc gcgtgccgca cgcgtggctg
      301 gaggcgacgg agggtatgtg accggccgct cgccccgggt cacgcggagc cggggcgttc
      361 cttgacgacg tagccctttc gagccttcga atcggcgagc ttcctcgcct ccgtctcggc
      421 agccgtcgac gtcgcatgcc gggccacgcg ttcctgcccc gccgttccga tgcggccgaa
      481 acggacgaag ctctcgcagt ctcggacacg gatctcccag aacttcgagg atcccccgcc
      541 gacgaactcc agataacgct ggaacgagaa gccatccagc tcgacgccca gtgtcttctt
      601 cagttccggc tcctcatcgg cgggcgaacc gctgacagga tcaggatcag gccccggctc
      661 gcgtcccggc tctggctctg gctccggctc gagttcaagc tcaggcgcgg attccggctc
      721 cggctcgggt gccgagctct gagccggcgc cacttccggt tcaacccctg cgaggcgagc
      781 ctccaggcgc aaaaggaccg cgtccggctc gaggagccac tcgcgggcga agacgaactc
      841 gcttcgccag ccgaaggagc ggagaagggc cggcttgagc acgtcccgcc cgaggcgctc
      901 ggtctcggcg aaatggcgtc tcgtgtcgag gaagacggcg aggcggtact cggcctcacc
      961 cggccgtcgg atcgcgaggg ggcagcgaaa gcgggacgtg ccgacgtcga gatcgacctc
     1021 gtatcccctg tcgcgcagac tctcggcgag ctgcacggcc gccgtctccg cctggaagct
     1081 ctccttccgc cgcgccggcg cgagctcgtc gaggactctc ctcgcgccct cggcgtcgcc
     1141 cttcgacgtc gcctcggcgt atcggaggta tcgcttgaga caggcggcgc cgtcgttgaa
     1201 gtcgttcgtg atgtccgcgc cgcgcatcga cgagacgacc gccatgtgcc gcttcgctct
     1261 cgaaaaggcc acgttcagcc gccgctctcc gccgctctgg ttgatcgggc cgaagttcat
     1321 ccggaccttg ccctgcgggt cccggccata gcagacgctc aggatcacga cgtcccgctc
     1381 gtctccctgg atgttctcga ggttcttcac gagcaggccg acgaactgat cgtcgtcctc
     1441 ccgctcgagc tcggcctcgt agcgtcgcgc gaaatcctcg tcggtccggg ccagctcctg
     1501 cagcgccccc tcgatctccc cctgctgcgc ctcggagaag gcgacgacgg cgatggttgg
     1561 ccgatccccg cgcagcagca gagctcgaac gagctccgcc acgtggcgcg cttctccctc
     1621 gttccggcgg tcgctgtaga cgccctcggc aaggtggtgg aaggagaccg gccgggcgag
     1681 gatcgcatcc gtgtcgatgc cggctccggt ccccgggtgc gggcagatct cgtccatcgg
     1741 gcggccggcg acgactctct cgggcacggt caggaggccg ttgccgtaga aggccgcgtt
     1801 cgagaagctg atgagcgact ccgaacggct ccggtagtgc cagccgagca tcgtcgccgg
     1861 caggctcttc tcggagtggt ggaggaggct gtcgctgtcg aggtcgtact ccacgacctc
     1921 cccgttctcc tcgaccgtga acgattcctc ctcggtgttc gccgccgaga agaagctcgt
     1981 tggcggaagc tgcatctggt cgccgacgac cacgacctgc cccgaccgga agaggcacgg
     2041 gacggcgtcc tcgagccgga tctggctcgc ttcgtcgaag acggtgacgt cgaactgcct
     2101 cgagtcgagc gggagcgcgt cggagacgct gagcgggctc atcagccaca ccggcttcag
     2161 gtcgaggatg accggccccg cctcgctttg gagcagctct cgaatcgagc ggaaccgcat
     2221 cgacttgccg agctcgcgct ccagctcctt ccgccccctc acgtaggcac gcttgcgcgc
     2281 cttctgatcg gccggcagcc ccgcgtcggg cagggaggaa agacgcacgt tctcgaagaa
     2341 ccggccgcgt gcccgctcga ggacggtccc ggcgttggca gcgagccacc gccggtacgc
     2401 ctcctcgacg cgccgcacgc cgaggctccg ctcgagcgac gagaatcggc cgagaactcg
     2461 atccgaccgc aacgcttcgc ggaacgcctt ccaggcaagc gcggcctcta cgccccgggc
     2521 ctcgcaggga aggtcccgga acgaatcggc aagcccggcc ggcagggccg cgagggcggc
     2581 gaggcaggag aggaactccg aaacctggct cagcgcctcg cgaatcccct cgagctgcgc
     2641 acggagctcg tcgaggccga gagattcggg gcgttcgacg acgccgtgca gcgcgtcccg
     2701 caggtccatc gccgaccggc ccgcggcacc gagctccccc agaatcgcga gtccgccccc
     2761 cgatcccgac aggagccttt catggagctc cctgatcggt ctcgacccgg ccgcgagctc
     2821 ggcccgcgcc tcgtcgaggc gacgccgcag ctcctccggc ttcccgagac cgaaccggct
     2881 ctcgatccgt tcctcgccct cgcgtgcggc cgcctcggcg cgatgctcct ccaggagcca
     2941 ctccaggacc tgcacgcggg tcggacggac cgccgacgaa cggaagtcgt atcgctcgcc
     3001 gaggaggcgt ctcaggcgcc accacgcagg cttgaggaac ccgagcgccc cctggacctc
     3061 ccgggcctga ccgagggccg cttcggtgtc gttcgggctg agcttcgatc tccagccgac
     3121 ggtcgcgtcc tttcggcgcg cgacgttcgc cacgagggcc tcctgctccg ccagcagctt
     3181 cgcggcctcc ttcgagagcg tgctgcccgg ctcgaggagc tcgagcgccc cccgctcgag
     3241 aaacggcgtt gccatcctgg agtaggcctc gagcgcgagg agggccgcga ggccatccca
     3301 ccctcgtctc ggcaggtccg aggcctccag ccggtcggtg atctccgcga ggagcgtccg
     3361 cgcctcggcc acggcgcgcg aaacgcgctc caccgggcgc gcttcgtcgg cgagcgccgg
     3421 gctcagccgt cgcaggggat gccgcgcgag gacgcccgtc ggctcgatct cctcgacgag
     3481 agactgcaaa cgccgaagcg ccccttcgtt ctcgacccat gcgcgatacg ccgggagacg
     3541 ctccgcctcg atcggatcca gcggcggcgc ggatgggccg atctcgagca gccgctcgat
     3601 caggccctgg accgcgatcc cggcgggctc ggggaccccg aggacggccc ggtcgtactg
     3661 ctcgatcggg gcgaggtccc gccggatgct ccggacgagg tcctcccgct cgcgctcgcg
     3721 acggcccggc tcgtccgacg ttcgggcgag ccagccttca taggtcgcct tcaggtcctg
     3781 gatgacggcc ttcttgtcct gctgcgagtc gtggacgagg cagacgatct cctcgagccc
     3841 cgactgcttg agccgatggt ggacgacgtc gatcgcggct ctcttctcgc agacgaagag
     3901 gacgcgcttt ccccgtgcga cgagctcggc gatcaggttc gtgatggtct gggacttccc
     3961 cgtgccgggc ggcccctgca cgatgagact ccgcccgttc gcgaccgccg cgatcgagga
     4021 cgactgcgtc ggatcgcacg gcacgaccgg gaaccgggcc tcgaacggct cctccggaag
     4081 ggcctcctcc ggtgcccgcg gcgcgaggga gaagacgcgg tcgaagctgt cgctcgggac
     4141 gtcctgcgac aggagcccct cgtagtcgcg aaccagcgac atcttccgag atctcaggtt
     4201 cgtcagcgtc aggctgcaga ggtcgaactc ccacgcatag gggttgcccg ccgcgtccgt
     4261 cttcaggtgg tagtggagcg aatcgcgctc ggccaccgcg cccggtgtca actcggggac
     4321 cgcgaagctt cgggtccggg gcgtctcgtc gaggagagac cggagccgcg tctccggtgc
     4381 tcgaacgcgg gcctcgaaca gcttcagccc gagcggcgcg aagctctcgg ggccgtagct
     4441 gtagtcgagg ctctggaacg acctcgcgga gcgtgccccc gagcgcgccc gccggcggta
     4501 ctggtcgagg cggcgccggg cgcggtcgtg gatcagctcg atgcgcgggt gctcgatctt
     4561 ctccaggctc acgccgggct cgctcgcctg gactcgcttc accaggaagt cgaagaaggc
     4621 gtcgaggtcc gtctcggcga ggtcgaggga ctcggggagg tcgatgccgt agctctggcg
     4681 aaagacgtgc cgcaccacgg ggttgacctc ggcctcagcc gtcagcggac gaagaagatg
     4741 gacgtcgcgc acgcccttct tcttctcgag ctccacgggg atgaggacga ggggcgactc
     4801 gaagcgctcc cgcgtctcct tcaggtcggt ccatcgaagc atgcagagga cgaggcggag
     4861 ctgcgagaag ccatactccg cacggtcccg gcgcgcctcg gcgcggatct gatcgagcac
     4921 gcccgggagg aagacgtggt cctcgaagcg gaggtagcgc ccgagcggaa tcgggtcgcc
     4981 gtcgacgacg agccggcgga gcacgggtcc ccatgtgagg agctgatccg gccggatgct
     5041 cctcacgtcg aggaccatcg gcacggaagc ctgggtcagg ttgacgctct gaagcgtgga
     5101 gcggaagtga aggagccggt tcctccgcgt gacctcgaag agcctctcct ggaggcgcga
     5161 gaggatcacc tgtcgccggc ccttcgggtc ccgctccttg aatccggcga cccgggcgag
     5221 gtcgaagtcg aagtcgactt cctggtcccg gtagttctcg agcgtcttcc cgaggcggcc
     5281 gagatcctgg ggccggcggt gccggtcgag ctccgtcatc ctcacgatcg ccttcgcgac
     5341 gacggggttc aggccggggt tgagacggaa gaggttctcg cgcgcggagg cgaatgtccg
     5401 gaggtcgccc tcctcaccga ggtcgagccc caggaggacg ctccccagga tcagcccgag
     5461 aacgaacgtg tcggtcagcg ggtcgtggtg gccgagcgtg tgctcccacg aacggaagcc
     5521 ggggacgtag cagcgccgct cgacgggccc cgtccccgcc gccaccgaca gcgactcgac
     5581 ctcggaccat ccctggtcga cgtccgtgac gaccttcgcg tggccgacaa cgtcgagggc
     5641 ctcgctctct cgcgcgccga gctcccccac cttcgcgttg cggcggatcg gtctcgcgag
     5701 cgcctccgga aaccaggccc gcccgccgtc gacgcggacc tcggcgagcc cctcgagagg
     5761 ggacacgagg cccctctcgt gcagcgcctc aacctggccg agaagcggca ggacgagggc
     5821 gagcgcgtcg tctgtcggaa gcggtccctc ttcgatgcgc acccgaagga aatcggacag
     5881 gagggccggc ccggtcacgc cgaggcctcc gcgacgaccc gggacacgag tcccgccgcg
     5941 ccggctctcg ccgccgcgag ggccttcttc gagagcccga gctccttcgc ggcgacttcc
     6001 gagaacctcg cctccaaccc cagctcggcg gcgaggacga gcccccgggc gagcggcacg
     6061 tcctcgagct cacgctccac ggccaccagg tcgaggacga cgtagcagaa gtagtcccgg
     6121 atcgacgcgt cgcacgtctc cacgaaccca cggagccggt ccaggtcgcc ctggctcctc
     6181 gcggacggcg agaagtcggg aaagaaccgc cgcgcgtggc cgacgacgct ctccgtgcgg
     6241 aacggcaccg ggccgaggta cgccgcgacg acgtcccggg tcagcccctc gacccggcga
     6301 cgggccacga ggtcgagggc gccgagggcc ggattcccct ccagcatgca cgcgacctcg
     6361 gagtctgctt tctccccctt cgattcccag agagcgagcg ctcgtgcgcg gatgaacgcc
     6421 tcggggtggg tcagctgctc ggcgcggggg ctgccctgcg cgaaaatctc gtcggcctga
     6481 cggaggtagc tctcggcgct gacctcggcg aggcccgttt ccaccttcag gagcgccgcg
     6541 atcgagtcgg cggcgttccc cgacgcgaga agcgaaccgc ggtcggcgaa gacctccgtg
     6601 tagaggtcgt agagccgggc gctctcgacg tgagacgggt cggcgctagc gtcgccggcc
     6661 atcgcctcga ggacctggct cgcgacgagg aaggccccct cttcgagggc gtagaggatc
     6721 gcgtgggaga gctcgtgacc gaggagcgcg cggagctcgc ccgggcggag ggaggagagg
     6781 accggcccct tgaggacgac gtgcgcctct ccgggaatcc aggcgaggaa ggcgttcatc
     6841 cccccttccg ccgcgggggc ctggtagatc gtcaggggta tctcgagtcc gagagccgac
     6901 tcgacctcgt ccgcgatcgc atacagctcg gcatgcccct cgcgctcgag ccggtaggtc
     6961 gacttgagga tgtcgagccg gacctgctcg gcgtgcttcg cccggaacct gttcgaggag
     7021 aaccactccc agatgccggg ttcctccgat cggaggtact cgacgatcgc tcgctggtac
     7081 gcgagaggcg tgaggcgcgg ggcggccact tcggcgatca cgggcggaga attctaggtg
     7141 atgcgcaccg gacgccgctc tcgcgcgtcg cgatcggcat ctccgcctcg accaggctcc
     7201 tatcggcggc gacaatcggc cttccgatga tcgactcacc ccgccccctc gccgcctggc
     7261 gggggccgat cagccccgcg cagtacgccc gcgtgccgca cgcgtggctg gaggcgacgg
     7321 aagagacgtg accgaacgcc gtcctggccc cgagggccct ccgaccgcga agacgcgacg
     7381 ccgggcgcgc cggccagtgc ccgggccggt ggcccaggcg aagctcgggg tgatccgagc
     7441 cgcttgcgga catgcctttc cggtcgccga cgtcgaacag ctcctcgagg agatcgagtc
     7501 gggctatcgg ggccccgcgt gattcgctgt cgatccttca aggtcgacct cttctccacg
     7561 cgctccctac ggccgcgcca cctcgagacg atccggacca aagccatccc ggtctggtcg
     7621 gtccgctcgc gttctcccgg aggacgtcct cgcga
//
LOCUS       DAOONL010000146         7614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11381_length_7614_cov_10.918375, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000146 DAOONL010000000
VERSION     DAOONL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11381_length_7614_cov_10.918375"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..331)
                     /locus_tag="PLP50_14930"
     CDS             complement(2..331)
                     /locus_tag="PLP50_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52887.1"
                     /translation="MPDADSLTLAAALLASGAKPPRVLETLAEVFEAYHGRDENGGPE
                     PHVVLSPQLAKFFRDLEAKHGMTAAAAREVACAVVAESRRGEPGPIRVEYVNDWRKDQ
                     PTKGDPE"
     gene            complement(334..687)
                     /locus_tag="PLP50_14935"
     CDS             complement(334..687)
                     /locus_tag="PLP50_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52888.1"
                     /translation="MSERDARGLFQPGNRVGVGRAPRRDGLNLARWFRQALSDPERRA
                     KLLARIDRELDGDGPAPVLVKALAYGYGEPRQVLEADLKVRASKLARLVGVDVETLIA
                     EADRLSKEAGYETEN"
     gene            complement(955..1149)
                     /locus_tag="PLP50_14940"
     CDS             complement(955..1149)
                     /locus_tag="PLP50_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52889.1"
                     /translation="MNPLIISVSTAWECSGLSLAAFGDLVDGLVTSGRAVRHRDILVF
                     PNVRTAALVLTGRPRRRPRR"
     gene            complement(1460..3622)
                     /locus_tag="PLP50_14945"
     CDS             complement(1460..3622)
                     /locus_tag="PLP50_14945"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HPA52890.1"
                     /translation="MPGDPPASPAEAARDLLRRGFQPLPLDPKSKGTRRPGWPDFRSS
                     PERVEADFPADGNLGLLLGEVSGGLTDLDLDSEEALRLAPTVLPRTDLVSGRASAPDS
                     HRFYLAPGAPYLKLDDPLRTGSKATLLELRSGTREAAHQTMVPPSVHPSGERVAWSRY
                     GEPATVPADDLERAARELAAASVLLRYLPERDGSQRRHSLFLALSGVLLRSGWEPERA
                     QRFERAIRKAAGDDSNREHAVDSTVKRLDRGATATGWPSFVAVLSETLPGGEADARKV
                     AEWLAATLALPAGNVRVLESAPTPGEWLPVPAEPEWPEPPDEAAFSGIFGDLVRAVAP
                     HTEADPVALLGHALVYFGAEIGRQPRVMVGATAHRGNLFVANVGSTARGAKGTAKAEV
                     EPVFREAGATVLDRVVSGLQSGEAVVHSIRDPKGKDEGEPDKRLILFEAELGRLFKVA
                     GRDGSTVSAVARELWDSPRILQTASKHFGEKATEPHGALLGHITDAELVLRLTNDDIS
                     NGFANRFVFISVRASKLLPEGGRVPQAVLQPLAARLRERLDLARTVGEVTRTAEARAA
                     WSAVYPALREERPGLVGALLARAAPQVLRLSLLYALGSGAARIEREHLESALALWSYS
                     ERSVRRIFGDLIGDPVADALIRALRAAGAAGLSRSELWVVLGRNGSVSRLERALDTLA
                     RLRLARGRIERTGGRPAVRWFAAEHVPAEAPAATEAAA"
     gene            complement(4019..4621)
                     /locus_tag="PLP50_14950"
     CDS             complement(4019..4621)
                     /locus_tag="PLP50_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52891.1"
                     /translation="MDDVTGPAWYREAWPSIGDAWGERGKTMMALLLGGEAEVSRWLV
                     SQGRAGDSELTPDDLLPLLKATERAPVSVRFWWALSLARSAQTMSVSAVSSTGEVTVR
                     SDRPIAPKLPSLVTVIREKRPDTFAAVMKILSTAKVLMPPHLRAVAIARVLSTGKAHP
                     HSEEAGETLKRRERRKAERAGMKLSAFKRGKPGRPRKSGQ"
     gene            complement(4646..5842)
                     /locus_tag="PLP50_14955"
     CDS             complement(4646..5842)
                     /locus_tag="PLP50_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPA52892.1"
                     /translation="MTKKVRRPRGSRLRNITNRFLESFATDKPGGLRVFDTETRGFAA
                     TAYPGGRIVFGVRFGNRVRRRWITLGELGTEVTLSEARARAREIVAESVLGGDPVGDR
                     RRAEAIPLLRAFLDDYAAWSEKTKKARTLRAEAVYRKVAEKRLGSVRLDEIEPAALRE
                     IALDYTKAGQLYSANRFLSFMSAVLSQAVKLGHIATNPAAAVPRNVEKPRKRVLSADE
                     LGRLWSALETEPDAGLRAAFVLMVVTGCRSSEAREARWSDLDLEAGVWTVPDTKAGEA
                     QEIVIPAAACELLKAIPKEGPFVCPGRFGDKPRADFWRVWKGIAARAKVSDATPHDLR
                     RTLSDLIRRSAGEAVAQAALRHTDSRTTVRHYSAADAGEIRETVARVLPFLAPKAKPA
                     RKRRRA"
     gene            6348..>7614
                     /locus_tag="PLP50_14960"
     CDS             6348..>7614
                     /locus_tag="PLP50_14960"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPA52893.1"
                     /translation="MTVEISFAFPLPGGLHARPAVRLQEEVARFRSVVTFLNRANGTS
                     ASARSVLALVATRTALGEPCALHVQGEDEAAAGAALRKFVKKELPGLDDGPPARVATG
                     APLPRVLARAEARVHRGAPASGGIFRGRAVVVGSRRATFDEERTRTEADELARLEAAF
                     EKAAAAIRVRLAAAEYETERAVLEAHLSIAEDPGLRARAAAAVVEGAAAPTAVFAAAS
                     HFAEIFRASGSTLLAERALDLHDVAEAVAHVLSGDGAPPPTVELGEDAVVVAARLGPG
                     QLLALPRARVRGLVLSEGGTLSHTVVLARAFGVPCVTGVEGVERALATGDDVVVDGER
                     GLVVVAPKGPVLRFYEAQRELLSTAARRLERFRTARGVTADGRRIEVGANVGTVEEAD
                     SAFENGAEGIGLFRTELMFLDRDEPPTEEE"
BASE COUNT         1042 a         2661 c         2802 g         1109 t
ORIGIN      
        1 ttcattcggg gtctcctttc gtcggctggt ctttccgcca gtcgttcacg tactcgacgc
       61 ggatcggtcc gggctcgccg cgcctggact cggcgacgac ggcacacgcg acctcccggg
      121 ccgcggcggc ggtcatgccg tgcttcgcct cgaggtcccg gaagaacttc gcgagctgcg
      181 gcgagaggac gacgtgcggc tcggggccgc cgttctcgtc gcgcccgtgg tacgcctcga
      241 aaacctcggc gagcgtctcg aggacccggg gcggtttggc gccgctcgcg aggagcgcgg
      301 cggcgagggt gaggctgtcg gcgtcaggca tcgtcagttc tccgtttcgt agccggcctc
      361 tttcgagagc cggtcagctt cggcaatgag cgtctcgacg tcgacgccga cgagacgcgc
      421 gagcttcgag gcgcggacct tgagatcggc ctcgaggacc tgccgcggct cgccgtagcc
      481 gtaggcaagc gccttcacga ggacgggcgc cgggccgtcg ccgtcgagct cgcggtcgat
      541 ccgggcgagg agcttcgccc tgcgctccgg gtcggagagg gcctgacgga accagcgcgc
      601 gaggttcagc ccgtcgcggc ggggagcgcg gccgacgccg acccggttcc ccggctggaa
      661 gagcccgcgc gcgtcacgct cgctcatcgg ctcaccttcg gctgtaagcc ggttcccgag
      721 ggcctcgggc agggcctcca cgcgacgggc ctcggagggc ctcccggcgc gattctggcg
      781 gtcgcccgga cgcggtcggc gcgaccgagg gcgacgcgat aggcgaaggt agagggcggt
      841 ctcatcgtgt cacctccaag attccggatc gggtttcgta tgcctcggtt cggccggata
      901 cggcgagggc cggcaggagg agcgccgcgg cgaggaggag gcggaggaag aacctcatcg
      961 gcgcggcctc cgacgagggc gacccgtgag gacgagcgcg gccgtccgga cgttcgggaa
     1021 gacgaggatg tcgcggtgcc tgacggcccg gccggacgtg acgaggccgt cgacgaggtc
     1081 gccgaacgcc gcgagcgaga gcccggagca ctcccaggcc gtcgagacgg agatgatgag
     1141 cgggttcatg gccgcctccg cctccgctcg cacggaacgc aaaccgcgtc gccgcgccac
     1201 gagacccgcc accaggagag gccgcagtcc aggcacgtcc ggccggcgag gcgcgccgcg
     1261 gcccgttcct gccgcgcgcg agcgacgaac gggaggacct tccgcgcctc ccgctcgcag
     1321 tcttcgcaaa gcacgtccgc ttccggtatg tcgccggctc cgcaacggga gcacggccgc
     1381 ggctccggcc agcactcgac cggcggcggc gggacggccg cacggaggag cgcgaggtaa
     1441 tcgggagccg cgctggagct cacgccgcgg cctccgtggc ggccggagct tcggcgggaa
     1501 cgtgctccgc cgcgaaccag cggacggcgg gacggccgcc ggtccgctcg attcggccgc
     1561 gggcgagcct gaggcgcgcg agcgtgtcca gagcgcgctc cagccgggaa acggaaccgt
     1621 tccggccgag gaccacccaa agctcggagc gcgagaggcc ggcggctccc gcggcccgca
     1681 gcgcgcgaat gagagcgtcc gcgaccgggt cgccgataag gtcgccgaag atccggcgga
     1741 cgctccgctc cgagtaactc cagagcgcga gcgcggattc caggtgctcc cgctcgattc
     1801 gggccgcgcc ggagccgaga gcgtacagaa gcgacaacct gaggacctga ggcgcggcgc
     1861 gcgcgaggag cgctccgacg agtccgggcc gctcctcccg aagcgcggga tagacggcgc
     1921 tccaggccgc gcgagcttcc gccgtgcggg tcacttcgcc gaccgtgcgg gcgaggtcga
     1981 ggcgctcccg gaggcgggcc gcgagcggct ggagcacggc ctgagggacc cggccgcctt
     2041 ccgggaggag cttcgaggcc cgaacggaga tgaagacgaa gcggttcgcg aagccgttcg
     2101 agatgtcgtc attcgtcagg cggaggacaa gctccgcgtc cgtgatgtga ccgaggagcg
     2161 ctccgtgcgg ctccgtggct ttctcgccga agtgcttcga ggccgtctgg agaatgcgcg
     2221 gcgagtccca gagctcgcgg gcgacggcgg agacggtgct cccgtcgcgg ccggctacct
     2281 tgaagagccg cccgagctcc gcctcgaaga gaatcagccg cttatccggc tcgccttcgt
     2341 ccttcccctt cgggtcgcgg attgaatgga cgacggcctc cccgctctgg aggccgctta
     2401 ccacgcggtc gaggacggtc gcgccggcct cccggaatac cggctcgact tcggccttcg
     2461 cggtgccttt ggcgccgcgc gcggttgagc cgacgttcgc gacgaagagg tttccgcggt
     2521 gcgcggtcgc gccgaccatg acgcgcggct gccggccgat ctccgcgccg aagtagacga
     2581 gagcgtgccc gaggagcgcg accgggtccg cctccgtgtg aggcgcgacg gcgcggacga
     2641 ggtcgccgaa gatcccggag aaagccgctt cgtccggagg ctccggccat tccggctccg
     2701 cgggaaccgg gagccattcg cctggagtcg gcgcgctctc gaggacccgg acgttaccgg
     2761 ccgggagagc gagagtcgcc gcgagccatt cggcgacctt ccgcgcgtcc gcctctccgc
     2821 ccggaagggt ctccgagagg acggcgacga aagacggcca gccggtcgcg gtcgctccgc
     2881 ggtcgaggcg cttgaccgtg ctatcgaccg cgtgctcgcg gttcgagtcg tcgccggccg
     2941 ccttccgaat cgcgcgctcg aagcgttgcg cccgctccgg ttcccatccg gaacggagga
     3001 gcacgccgga gagcgcgagg aagagggagt gtcgccgctg agagccgtcc cgctccggga
     3061 gataccggag gagcacggag gccgccgcga gctcgcgggc cgcccgctcc aggtcgtccg
     3121 cgggaacggt cgccggttcg ccgtagcggc tccaggcgac ccgttcgccg ctcgggtgaa
     3181 cgctcggcgg gaccatcgtt tggtgggcgg cctcgcgggt ccctgagcgg agctcgagga
     3241 gtgtcgcctt cgagccggtg cggagcgggt cgtccagctt cagatacggc gctccaggcg
     3301 cgaggtagaa ccggtgcgag tccggagcgc tcgcgcggcc ggagacgagg tcggtccgcg
     3361 ggaggacggt gggagcgagc cggagcgctt cctcggagtc gaggtcgagg tccgtcaggc
     3421 cgccggagac ttcgccgagg aggagcccga ggttcccgtc cgccgggaag tcggcctcga
     3481 ctctctcggg tgaggaacgg aaatccggcc aaccgggccg gcgcgttccc tttgatttcg
     3541 ggtcgagagg gagcggctgg aagccgcgcc ggaggaggtc gcgggccgct tcggcaggag
     3601 aggcgggcgg gtcaccaggc acgggaagct tcctcccggt cggccgcggc ggaggtacac
     3661 tccgcatagg cgctgttcgt caggttcatg cgcttgcctt tctgggagcc gtccgttgca
     3721 ccggacggct cgctttgttt tcggaccgct cggcggcccg tctctcagcg gtcgaggaga
     3781 aactccggcg ggcttcgagc cattcgcgga ggtccggggc gaggtagtag acccgccggc
     3841 cttcgtagcg gaagcgcggg ccgcctcccg tcacgcggag cttgcggagc gtttgcggac
     3901 ggagccccac ggcgggcgcc gcgtccttcg tccagagccg gagcctttcg tctaccttca
     3961 cggaacgaag ggtcaacgaa cgacgacggc gataaaaggg acgtttcgag gggggtgcct
     4021 actgaccgga ttttctcggg cgccctggct tccctcgctt gaaggccgag agcttcatcc
     4081 cggctcgctc ggccttgcgt cgctcgcgtc gcttcagggt ctctccggct tcctccgagt
     4141 gagggtgagc cttgcccgtc gagagcacgc gggcgatcgc cacggcgcga aggtgcggcg
     4201 gcatgaggac cttcgcggtg ctgaggatct tcataaccgc ggcgaacgtg tcgggtctct
     4261 tctcgcgaat cacggtcacg aggcttggga gcttcggagc gatcggccgg tccgatcgca
     4321 ccgttacttc tccggtcgat gagaccgccg agacgctcat ggtctgagca ctccgcgcga
     4381 gcgagagtgc ccaccagaac cggacgctga ccggcgccct ctccgtcgcc ttcagaagcg
     4441 gcaatagatc gtccggcgtc agctccgagt ctcccgcgcg gccctgcgac acgagccaac
     4501 gggagacctc ggcctctccg ccgaggagga gcgccatcat cgtcttccca cgctcgcccc
     4561 aggcatcgcc gatgctcggc cacgcctcgc gataccaggc cggccccgtt acgtcgtcca
     4621 tcgtgcctcc cttctgaccc ccgcctcacg ctctcctccg cttccgcgcc ggcttcgcct
     4681 tcggcgcgag gaacgggagg acccgcgcga ccgtctcgcg aatctcgccg gcgtccgccg
     4741 cgctgtagtg acgaacggtc gtccgcgagt ccgtgtgccg gagcgcggcc tgagcgacgg
     4801 cctcgccggc ggagcgccga atcaggtcgg agagcgtccg ccggaggtcg tgcggcgtcg
     4861 cgtccgaaac cttcgcgcgc gcggcgattc ccttccagac tctccagaag tccgctcgcg
     4921 gcttgtctcc gaaccggccc ggacagacga acgggccttc cttcggaatc gccttcagaa
     4981 gctcacacgc cgcggccgga atgacgatct cctgagcttc gccggccttc gtgtccggga
     5041 ccgtccagac cccggcctcc aggtcgaggt cgctccagcg ggcctcccgc gcctccgagg
     5101 agcggcaacc ggtgacgacc atgaggacga aggccgcccg gaggccggcg tccggctccg
     5161 tttcgagcgc gctccagagc cgcccgagct cgtccgcgct gaggacccgc ttccgcggct
     5221 tctcgacgtt cctcgggacg gccgcggcgg gattcgtcgc gatatgcccg agcttcacgg
     5281 cctgggagag cacggcggac atgaaagaca ggaagcggtt cgcgctgtaa agctggccgg
     5341 ccttcgtgta gtcgagcgcg atctcccgga gcgcggccgg ctcgatctcg tccaggcgga
     5401 cactcccgag gcgcttctcg gcgaccttcc ggtagacggc ctcggcgcgg agcgtcctcg
     5461 ccttcttcgt cttctcgctc caggccgcgt aatcgtccag gaaggcccgg aggagcggga
     5521 tagcttccgc gcgcctccgg tcgccgaccg ggtctccgcc gaggacgctc tcggcgacga
     5581 tctctcgggc gcgagcgcgc gcctcggaga gcgtgacttc cgttccgagc tcgccgaggg
     5641 tgatccaccg gcgacggacc cggttcccga accggacgcc gaagacgatc cggccgccgg
     5701 gataggccgt cgccgcgaag ccgcgggtct ccgtatcgaa gacccggagg ccgcccggct
     5761 tgtcggtcgc gaagctctca aggaaacgat tcgtgatgtt ccggaggcgg gagccgcgag
     5821 gacggcggac tttcttcgtc aaattcgtct ttttcgtacc ttcaagagat ttgctctcct
     5881 gttcgcccgc tccgtcctcg cccttcctca tggctcctcg gcctccgttt cgcccgtgcc
     5941 acgggtcttc ctgtcgtacc accttcgtac cacaaacgag tctacgatac gggaacacga
     6001 agtcaacgga aagcggctta tttagcggag tctttcgctc gcgggtcgcg tacctttgag
     6061 ccgtgcggtt tgactacgga tcaaaaggtt gggggttcga gtcctcctgg gcgcaccacc
     6121 ccctttccct ggttgcgaag aggatcgcga tcggaccccg gtagccccgg ggtcttcgct
     6181 tctccgcgcc gcctccgcct tcttcaggat ggagggggag gagaaggccc gtgcgtcgct
     6241 tgctagactg acggaccgaa tcccggaacg ccgacgcagg gggagcgaat gacgggacgc
     6301 ctcgccgggc aggggcggtc aggtccgggg cgcgggcggc ggtcgccgtg acggtcgaga
     6361 tctcgttcgc gttcccgctc ccgggcgggc ttcacgcccg gccggctgtc cgcctccagg
     6421 aggaggtggc gcgcttccgg tcggtcgtca cgttcctgaa ccgcgcgaac ggcacctcgg
     6481 cgagcgcgcg gagcgtcctc gccctcgtgg cgacgcggac cgcgctcggc gagccgtgcg
     6541 ccctgcacgt gcagggggag gacgaggccg cggccggggc cgcgctccgg aagttcgtga
     6601 agaaggagct cccggggctc gacgacgggc cgccggcgcg cgtcgcgacg ggcgccccgc
     6661 tcccgcgcgt cctcgcgcgg gccgaggcgc gcgtccaccg cggggcaccg gcgagcggcg
     6721 ggatcttccg cgggcgcgcg gtcgtcgtcg gctcgcgccg cgccacattc gacgaggagc
     6781 ggacccggac ggaggcggac gagctcgccc gcctcgaggc cgcgttcgag aaggcggcgg
     6841 ccgcgatccg ggtgcgcctc gcggcggcgg agtacgagac ggagagagcc gtcctcgagg
     6901 cccacctctc gatcgccgag gatcccgggc tgcgcgcgcg cgccgcggcc gccgtcgtcg
     6961 agggggccgc cgcgcccacc gcggtcttcg ccgcggccag ccacttcgcc gagatcttcc
     7021 gcgcgagcgg gagcacgctc ctcgccgagc gggcgctcga cctgcacgac gtcgccgagg
     7081 ccgtcgccca cgtcctgtcc ggcgacggcg ccccgccgcc gaccgtggag ctgggggaag
     7141 acgcggtcgt cgtcgcggcc cgcctcggtc cggggcagct cctggcgctc ccgcgggcgc
     7201 gtgtccgcgg cctcgttctc tcggaagggg ggacgctctc gcacaccgtc gtcctcgccc
     7261 gcgcgttcgg cgtcccgtgc gtgacgggcg tcgagggagt cgagcgggcc ctcgcgacgg
     7321 gcgacgacgt cgtcgtcgac ggggagcggg ggctcgtcgt cgtcgccccg aaagggcccg
     7381 tgctccgctt ctacgaggcg cagcgcgagc tcctttcgac ggccgcccgg cggctcgagc
     7441 ggttccggac ggcgcgcggc gtcacggcgg acggacggag gatcgaggtc ggggcgaacg
     7501 tcgggaccgt ggaggaggcc gactccgcgt tcgagaacgg ggcggaggga atcggccttt
     7561 tccggacgga gctgatgttc ctcgaccgcg acgagccgcc gaccgaggag gagc
//
LOCUS       DAOONL010000147         7530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11544_length_7530_cov_11.799465, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000147 DAOONL010000000
VERSION     DAOONL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11544_length_7530_cov_11.799465"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLP50_14965"
     CDS             complement(<1..250)
                     /locus_tag="PLP50_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52894.1"
                     /translation="MSFLDALGRIALDALEFHGRWVELMALTVRGFFRRPFDGRAIAT
                     QVVRVGVEGLPVVLLTAVFTGAVLALQTYTGFQRFHAEA"
     gene            complement(419..592)
                     /locus_tag="PLP50_14970"
     CDS             complement(419..592)
                     /locus_tag="PLP50_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52895.1"
                     /translation="MFKITEEPGTDGVRVRLAGDLTGTGFRIREDFWSKRSSFYASGR
                     VLIGLALEPLGPS"
     gene            1085..1243
                     /locus_tag="PLP50_14975"
                     /pseudo
     CDS             1085..1243
                     /locus_tag="PLP50_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1301..2506)
                     /locus_tag="PLP50_14980"
     CDS             complement(1301..2506)
                     /locus_tag="PLP50_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52896.1"
                     /translation="MYLTSLTHRDMLFDISQNWLHGNVDPGDGAFITAAFLLEHAITA
                     PSVTGLLEWTFGSHLPGELRFRHVRTKEQVRRSIIRGWTSPTPRARGLFEEFERLPEA
                     FFPTTPVDTIVAIAPDDGLVGMVRFKSLFRIADKVARGAARRLRADVESILLAGTHGG
                     ALSDLASVPGFAAALQEVNQTFLNGRARLSRDDLRVDDAIGAKLILPEEDMEAFEHRL
                     ASHPNVLSVQHREHHGPYNDVHLLAEVRCPPGGRTIDSLRAMDWRASAGRGVSAQQLR
                     DAVPQYVETGAKTFFLEVLLTTLPELVESEFGRGLHEERIARQRLDPLWSSRVATNVQ
                     LTTLSMLLLALAPTITIEEPPGKMSGRYLPETALGLLAQLFGLGVARSPFWVPEAIPD
                     ISCHGPDAA"
     gene            complement(2497..3129)
                     /locus_tag="PLP50_14985"
     CDS             complement(2497..3129)
                     /locus_tag="PLP50_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA52897.1"
                     /translation="MTIGSSRAGRERLVASAERLFPERGLRGVSVRELIADAGATRPL
                     LYHHFGSKEGLCREAVRRSVRRLDAALAAAASSSGSATERLARDGKVHAEAVHARALA
                     LDRQAPGVRESVVGLVRDLLVDALGSIAPDDHELDGTRLAVVAAAEFSARLSDRGTGD
                     GSLDDLTRMLTTVVRGARGRGRPEASPACTARAATDSTSGASLPEAHPCT"
     gene            complement(3126..>3458)
                     /locus_tag="PLP50_14990"
     CDS             complement(3126..>3458)
                     /locus_tag="PLP50_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52898.1"
                     /translation="PDPAGMGVAIDPVLPPASRAGDKWRLYATVTGRATYMAHHVIAE
                     GGATEDGPDHPGVEYVRPQLEAIVGLHVECWRLGLSVTGYRYLGEDTDLAHGTSRDWV
                     ALALNVRY"
     assembly_gap    3459..3558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3559..3933)
                     /locus_tag="PLP50_14995"
     CDS             complement(<3559..3933)
                     /locus_tag="PLP50_14995"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HPA52899.1"
                     /translation="MRHHSPLLPVVLALFAAATAAFAAEPPDHRGRLFRFEFDNDTFL
                     GSDDAFTAGWSLQLHTATQETWTGPARRSIALIPGLGDDGQGGRVVRRGYTLSQQIVT
                     PNDITIAEAQPDDAPWAGVLALS"
     gene            complement(4452..6539)
                     /locus_tag="PLP50_15000"
     CDS             complement(4452..6539)
                     /locus_tag="PLP50_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA52900.1"
                     /translation="MRRDGRTNPDVLTEEQVVSLIGVGTKLGAIRQPEELIRCLAQEL
                     RSLLVFDYLSLYLEGVLGEGPSWHVLDVERQSVLTPAIETPIEGPIGSWVLEHQIPYS
                     IPAAEGGPRFAEFEDEVASHGVASMVAVPLTTVRRRLGTLAVASRSPGAYGEGERRSL
                     SHVGSFVALAMDDAANFERLRVAQAELSRSNEGLRLILDVGRRLLSNLEMEALLRSIA
                     STVRASLQCEAAAIILVDKELTQGRVFVLDFPESRGVTPEGTSIPIAGTAIEEILRVG
                     GPCDLRSLPPDEPALCSLRAEGLAGGYSFPLVGRNRVLGVFAVAMREEGGFDAHRLEL
                     LEQVAGQVATAIDNALAYREIAELRDKLAGEKLYLEDEIKNELSFGEIVGRSEALRRV
                     LSRVETVAPTGSTVLITGETGSGKELIARAIHDLSGRQGGTFVKLSCAAIPTGLLESE
                     LFGHQKGAFTGAIADRVGRFELANGGTIFLDEIGEVPLEIQPKLLRVLQEREFERIGT
                     SRTRRTDARLVAATNRDLAAMVQERTFRADLYYRLNVFPIHVPPLRERPTDIPLLVRH
                     FVQQLERRLNKRVETIPSKTIAALTRYAWPGNIRELQNLIERAMILTHGPVLQVPLGD
                     LDLRETPAGAPVGMTRETAERAHLLATLADTDWVLAGAKGAAARLGMKRTTLQYRMKK
                     LGIVRPERPSAAE"
     gene            6685..7134
                     /locus_tag="PLP50_15005"
     CDS             6685..7134
                     /locus_tag="PLP50_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPA52901.1"
                     /translation="MRRPYAGLKINGATRAVQVVIGWAVELDVLDRRPANKNVTVLET
                     LKRRQVVSDFVFLDEDNKPLAKEKVAREFAMAKGVAGIPRRLRIHDLRHTFTSTLVSS
                     GVNLKVVSNLLGQADIATSARYARGDERVLGEVANVLNRVNDASAGK"
     gene            complement(7288..>7530)
                     /locus_tag="PLP50_15010"
     CDS             complement(7288..>7530)
                     /locus_tag="PLP50_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52902.1"
                     /translation="STYALTQDFDDAGLRLPGRDLFVRTVPFFEWLHSHPRLRSRAET
                     LADRDQRREANGGVLRPGPRSNRPQLTGPRGSAFDH"
BASE COUNT         1273 a         2423 c         2502 g         1232 t
ORIGIN      
        1 aggcttccgc gtggaaccgc tggaagccgg tgtaggtctg gagggcgagg accgcgccag
       61 tgaagacggc cgtcaggagg acgaccggga gcccctcgac gccgaccctc acgacctggg
      121 tcgcgatggc gcgaccatcg aacggccgac gaaagaaccc gcggacggtc aacgccatga
      181 gctcgaccca gcggccgtgg aactccagcg catcgagcgc gatccttcct aaggcgtcta
      241 ggaagctcat tggtccgccg accgaaacct gtacccggcc gcggttgctt tccgggacca
      301 cttccccggc gatgacacac gccacggccg cgctagtcgg tccggggtgc cctcgtcgtg
      361 gtactcgggg cgcgagagga cgatcacgac gtaagcgttc tgcgcgatcg ggcccagttc
      421 acgaaggtcc gagcggttcc aaggctagac cgatcaggac gcgaccgctg gcgtagaacg
      481 agctcctctt cgaccagaag tcctcccgga ttcggaatcc cgttccggtg agatcacctg
      541 cgagtctcac tcgaactccg tcggtgccgg gctcctcggt gatcttgaac atcgtcgtag
      601 cctcgcgacc ggagatcgca aggcggatgc cgagagcggg taacccttga gtgctcccct
      661 taagcccctg cgaaatagat ggttagccac acatttcaag ccgcgacagc ggcgaacgtg
      721 ccagctgcga agtgccgact gttcggcagc tgaggcccgg cgaacgagtg ccttcagtac
      781 tcaccgatca gagagcggcc gttcgaaaca ggaatgcccg catcgagccg acggagcggg
      841 tcggtgccca ccctcagtgc ccgctattcg gcagcacgtc gccgttctgg gcggagtagt
      901 cgcgtccgcc cacgtggtgt tcgagagatc gtcttttcag cagcagcgcc ggtgggatca
      961 gagggaaccg ccgcagcgtt ctcctcggat tccgcaggtc gggtcttcgc gcggcggggg
     1021 agccacggcc cgctgtctga aaggtctcga gtgctgactt cgagtcctca ggaggccgac
     1081 gaccgtgacc cagcccagca tctcgctccc catcgccctg cgcaagagcc ccgatcaggc
     1141 cagcccggac cttcttcgtg agcttcttcg gatgttcctc gagaagctga tgggcaacga
     1201 gaccggctct ttctgcggcg ccgactacgg cgagcgcgcc gaggatcaca tcaactccgc
     1261 gcggtccccg atgcccagct cgtctgacct atcggctggc tcacgcggcg tctggtccgt
     1321 gacacgagat gtccgggatt gcctccggca cccagaacgg gctgcgtgcg acaccgagcc
     1381 cgaagagctg cgcaaggagt cccagtgcgg tctccggcag gtagcggccc gacatcttcc
     1441 cgggcggttc ctcgatcgtg atcgtggggg cgagcgcgag gagcaacatc gacagggtcg
     1501 tgagctggac gttcgttgcg acgcggctgc tccacaaggg gtcgagccgt tgccgggcga
     1561 tgcgctcctc gtgaagtccg cgaccgaact ccgactcgac gagttccggc aacgtggtca
     1621 agaggacctc gaggaagaac gtcttcgctc ccgtctcgac gtactggggg accgcgtccc
     1681 gcagctgctg cgcagagaca ccacgaccgg cactagcgcg ccagtccatc gcgcgcagac
     1741 tatcaatggt ccggcctcca ggcggacagc ggacctcagc caggaggtgc acgtcgttgt
     1801 agggcccgtg gtgttcccta tgctgtaccg agagcacgtt cgggtgggag gcgagccggt
     1861 gctcgaaggc ctccatgtcc tcctcgggga ggatcagctt cgcgccgatc gcgtcgtcta
     1921 cccggaggtc gtcacgcgag agtcgcgccc gcccattgag gaaggtctgg ttcacctcct
     1981 ggagcgctgc ggcgaagccg ggcacgctcg cgaggtcgct cagcgccccg ccgtgcgtgc
     2041 cggccaagag gatcgactcg acgtcagcgc gaagtcggcg cgcggccccg cgtgcgacct
     2101 tgtccgcgat gcgaaacagg ctcttgaatc ggaccatccc gacgagaccg tcgtccggcg
     2161 cgatcgccac gattgtgtcg acgggggtcg tcgggaagaa cgcctccggc agccgctcga
     2221 attcctcgaa caggccgcgc gcgcgggggg tcggcgacgt ccaaccacgg atgatgctgc
     2281 ggcggacctg ttccttggtc cggacgtgac ggaaccgcag ttcgccgggc agatgagaac
     2341 cgaacgtcca ctcgagaaga cccgtcaccg atggcgcggt gatcgcgtgc tcgagaagga
     2401 atgccgcggt aatgaaagcg ccgtcgcccg gatcgacgtt accgtggagc cagttctggc
     2461 tgatgtcaaa gagcatgtcc cgatgtgtga gactcgtcag gtacatggat gtgcctcggg
     2521 cagggaagcc ccggacgtgg agtcagtagc cgcgcgcgct gtgcacgccg gcgacgcctc
     2581 gggccggccg cgtccgcggg cgccgcgcac gacggtggtc agcatccggg tcaagtcgtc
     2641 aaggctgccg tcgccggtgc cgcggtccga gaggcgcgcg ctgaactcgg ccgcggcgac
     2701 gacggcgagt ctggtgccgt ccagttcgtg gtcgtcgggc gcaatcgacc ccaaggcgtc
     2761 gaccagcagg tcccgaacaa ggcctacgac actttctcgt acccccggag cctgacgatc
     2821 gagggccagt gcgcgcgcgt ggaccgcctc cgcgtggacc ttgccgtcgc gcgcgagccg
     2881 ttccgtcgcg gatccggagc tcgaagcggc ggcagccagc gcggcgtcta agcgtcgcac
     2941 gctgcgccgc acggcctccc gacagagccc ctccttgctt ccgaaatggt ggtagagcaa
     3001 gggacgcgtc gcgcccgcgt cggcgatgag ctcgcggacg gagacgccgc ggagcccccg
     3061 ctcggggaag agccgttcgg cggacgcgac caggcgctcc cttcccgcgc gcgacgagcc
     3121 tatcgtcaat acctcacgtt gagggcgagc gcgacccagt cccgcgacgt gccgtgcgcc
     3181 aggtccgtgt cctcgccgag gtagcggtag ccggtgacgc tcagtccgag gcgccagcac
     3241 tcgacatgga ggccgacgat cgcctccagc tgcggccgga cgtactcgac ccccggatgg
     3301 tcggggccgt cttccgtggc gcctccctct gcgatgacgt ggtgcgccat gtacgtcgcg
     3361 cggccagtga ctgtggcgta cagccgccac ttgtcgccgg ctcgggatgc cggcggcagc
     3421 acggggtcga tggccacgcc catccccgcg gggtcgggnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnngg acagcgcgag gactccggcc cacggcgcat cgtccggctg
     3601 ggcctcggcg atcgtgatgt cgttcggcgt cacgatctgc tgggagagcg tatagccgcg
     3661 tcggacgacc cgcccacctt ggccgtcgtc gcccagtccc ggaattagcg cgatcgagcg
     3721 cctggcggga ccggtccatg tctcctgcgt ggcagtgtgc agctgcaggc tccagccggc
     3781 ggtgaaagcg tcgtccgagc cgagaaaggt gtcgttgtcg aattcgaaac ggaacagccg
     3841 gccgcgatga tcgggcggtt ctgcggcgaa agccgcggta gccgcggcga acagcgcaag
     3901 cacgacgggg agcagcggcg agtggtgtct catctcaatc cctggggcgc acattcggac
     3961 gccggtctga tgaatcaggg cgagtaggaa gcgcccagcg tcgtccacgc agcagacccc
     4021 ggaatggtcg aacgggacgc gaccgctggc gtaagacgaa ctcgtcgccg accagaaatc
     4081 ctccacgact tggactcgcg ttctggtgag atcacctttg agtctcacca agaacctcgt
     4141 cggtgccggg ctcctcggtg agcttgaaca tcgtcgtagc ctcgggacct gagggcgcaa
     4201 gggagatgcc gaagggggag aacccctgag cgacaccttc aagtctctgc ggaatagatg
     4261 gttagcgcac aactgaagaa gcgacggtgg cgcgagtgcc agccgcggtc tgccgactgt
     4321 tcggcagctc aggcccggcg ggtcactacc ttcggcacgt gccgatcaga aagcggccgt
     4381 acgaaacagc agtgccggcg tcgagctggg gcgagccgag ggagggggac ggtacccttc
     4441 tcctgtgccg gctattcggc agccgatggc cgttctgggc ggacgattcc cagtttcttc
     4501 atccggtact gaagcgtggt gcgtttcatc ccgaggcggg ccgccgcccc cttcgctcca
     4561 gcgagtaccc agtcggtgtc ggccagcgtg gcgaggaggt gcgcccgttc cgccgtctcg
     4621 cgcgtcatgc ccaccggcgc gccggcgggc gtttcacgca agtcgagatc tcccagcggg
     4681 acctgtagga cgggtccgtg ggtcaagatc atcgcccgct cgatcaggtt ctggagctcg
     4741 cggatgtttc ccggccaggc gtacctcgtg agtgccgcga tcgtcttgga gggaatcgtc
     4801 tccaccctct tgttgagccg tcgttcgagc tgctggacga aatgccggac gaggagcgga
     4861 atgtccgttg gtctctctcg cagagggggc acgtggatgg gaaagacatt cagccggtag
     4921 tagagatcgg cacgaaaagt gcgctcctga accattgcgg cgaggtcccg gttcgtggca
     4981 gcgacgagcc gagcgtccgt tcggcgcgtt ctcgaggtcc cgattcgctc gaactctcgc
     5041 tcctgcagga cgcggaggag ctttggctgg atctcgagcg ggacctctcc gatctcgtcg
     5101 aggaagatcg ttcccccgtt cgcgagctcg aaacggccca cgcgatcggc gatcgccccg
     5161 gtgaacgcac ccttctgatg accgaacagt tcgctttcga ggaggcccgt cggtatggct
     5221 gcgcagctga gcttgacgaa ggtcccgccc tgccgtccgc tcaggtcgtg gatggcacgg
     5281 gcgatcagct ccttgcctga accggtctcg ccagtgatta ggaccgtcga accggtcggc
     5341 gcgaccgtct cgactctgct caggacgcga cgcagtgctt cgctcctgcc cacgatctcg
     5401 ccgaagctca actcgttctt gatctcgtcc tcgaggtaga gcttctcccc agcgagcttg
     5461 tccctcagct cggcgatctc gcgataagcg agggcgttgt cgatcgcggt ggcgacctgg
     5521 ccagcaacct gctcgagcag ctcaagccga tgggcgtcga agccgccctc ctcgcgcatt
     5581 gctaccgcga agacgccgag aactcggttt cgaccgacca gaggaaagga gtagccgcca
     5641 gcgagccctt cagcccggag actgcacaga gcgggctcgt cgggaggaag gcttcgtaga
     5701 tcacagggac cgccaaccct caggatctcc tcgatggccg tacctgcgat cggaatcgag
     5761 gtcccctccg gcgtgacgcc ccggctttcg gggaagtcga ggacgaagac gcggccttgc
     5821 gtcaactcct tgtcgacgag aatgatcgcc gcagcctcgc attgaagcga agctctgacc
     5881 gtcgacgcga tggaacggag gagagcctcc atttcgaggt tcgacaggag acgcctaccg
     5941 acgtccagga tgaggcgaag cccctcattg ctccttgaaa gctccgcttg cgcgacgcgt
     6001 aggcgctcga agttcgcggc gtcgtccatc gcgagcgcga cgaagctccc aacgtgcgat
     6061 agagagcgcc tctctccctc cccgtaggca cccggcgagc ggctagcgac cgcaagagtt
     6121 cccagccgcc tgcggaccgt cgtcaacggg accgcgacca tggaggctac gccgtgactc
     6181 gcgacttcat cctcgaattc tgcgaagcgt ggaccgcctt ccgccgccgg aatggagtac
     6241 gggatctggt gctcgagcac ccacgatccg attggcccct cgatcggagt ctcgatggca
     6301 ggtgtcagta ccgactggcg ctcgacatcc agaacgtgcc agctcggccc ctctccaagc
     6361 acgccttcta ggtagaggct caggtagtcg aagacgagga ggctgcgtag ctcctgggcg
     6421 aggcaacgga tgagctcctc gggttggcga atcgcgccga gcttcgtccc gacgccaatc
     6481 agggagacga cctgttcctc tgtcaggaca tcggggttcg tccgtccgtc gcggcgcatc
     6541 ggcgatctcc tcggggatga ggatagtcga tgaatcgaag atcgattctg tttgggcgta
     6601 gctaagtgat ttgtttgtag tgggctgcgc gagatccaac gatgcgagcg gaccgctcgg
     6661 cccgagtgca gcgaaagtgg gtgcgtgagg cggccctacg ccggattgaa gatcaacggc
     6721 gcgactcgcg cagttcaggt cgtcatcggc tgggcggtcg agctcgatgt cctcgaccgg
     6781 cgccccgcga acaagaatgt gaccgtgctc gagaccctga agaggcggca ggtcgtctcg
     6841 gatttcgtct tcctcgacga ggacaacaag ccgctcgcga aggagaaggt cgcccgggaa
     6901 ttcgcaatgg cgaagggcgt ggctggcatc ccccgccggc tccgcatcca cgaccttcgc
     6961 cacacgttca cgtcgaccct cgtctcgtcc ggtgtgaacc tcaaagtcgt ctccaacctt
     7021 ctcggacaag ccgacatcgc gacgtcggcg cgttacgccc gtggggacga gcgggtcctc
     7081 ggcgaggtgg ccaacgtcct caatcgcgtg aacgacgcgt cggccgggaa gtagggagat
     7141 ccgaacagcc tcacggacgg cgccatgccg cccgtgaggc gaaactcgga gcgccctcgt
     7201 cctccgtctc gatctgccag cagtacagca gccgcgacgg aacgggcgct cagagtcgtt
     7261 ttctacagtc ccgctatacc agggacctca gtgatcgaat gcgcttcccc gagggccggt
     7321 gagctggggc ctattcgaac gcggaccggg gcggaggacc cctccgttgg cctcgcgtct
     7381 ctggtcccta tcggccagcg tttccgcccg agaccgcagc cgcgggtggc tgtgaagcca
     7441 ctcgaagaag gggaccgtcc ggacgaagag gtcccggccg gggaggcgga gtccggcgtc
     7501 gtcgaagtcc tgggtgaggg cgtacgtgga
//
LOCUS       DAOONL010000148         7460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11728_length_7460_cov_19.019581, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000148 DAOONL010000000
VERSION     DAOONL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11728_length_7460_cov_19.019581"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLP50_15015"
     CDS             <1..180
                     /locus_tag="PLP50_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52903.1"
                     /translation="HHNHVAMELLHTAWKARDSLTDSDALVGISLLSSRPAGLRRLQE
                     AVGRDAGTTENGENE"
     gene            177..2054
                     /locus_tag="PLP50_15020"
     CDS             177..2054
                     /locus_tag="PLP50_15020"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HPA52904.1"
                     /translation="MTRRVRRLLLTPALLLAPLFGAGRLEAQPVAPAARPAAADTAAL
                     VERHVAWLGGWPALDAMKDLSLDGTIRVAGLSGSLAVRARRDGRQRTEVDLKVLRSVE
                     VLAGSEGWERNASGQIEPMGLDKVRLQRRALDRTFGRHLRGEGVVTSLAGTEARGGRS
                     WSVLRFTYPDGDAYDLLVDPETGESRYERSVQDGRTTWTSSADVRVVKGIRVAARQEI
                     EGETALQAQSLAWESIAVNGGLADALFVPPAPAGSLVRLPAGRTTTEWVPVDLYRKRY
                     VFLAGELNGVPTDVVLDSGAGMTVLDRALAEKLKLRPEGELEARGTGGSVGAGLVSGL
                     TLKVAGVEVGPISAAVIDLSDVGRRLGRPLPVILGKELFHALVVDLDYPGLRLRLHDA
                     ATFRYEGPGRKVDLLPAEDGHKSLRISIEGDEPVVVGLDTGQGGALSVFRHYADARRL
                     LEGRPVSERRGGGVGGATLSKLATLRSVSVAGYDLRNVPATFQTTDVGGAFDTKRQEG
                     NLGAGILARFRVLFDYARSALWLEPGPGLAAPFPKDKTGLDLDWADGALVVEHVAPGS
                     PAAATGWKEGERVTALDGEPASPDSWRTELRWAEAEAGTKVRLTLADGTERTLVLAEY
                     Y"
     gene            complement(2062..2742)
                     /gene="can"
                     /locus_tag="PLP50_15025"
     CDS             complement(2062..2742)
                     /gene="can"
                     /locus_tag="PLP50_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HPA52905.1"
                     /translation="MKHLPNLFENNRAWADGLRRNDPEFFTRLCRQQAPPYFWIGCSD
                     SRVPANEIVGLLPGELFVHRNVANLVVQSDLNGLSALQFAVDVLRVKHVVVCGHYGCG
                     GVLAALRGERHGLVDNWLRHVADVREKHDAQLGRLGPERAVRDRLCELNVVEQVRNVA
                     QTTVVRDAWSRGQELSLHGWVYALEDGLLRDLGVSARTEAELAEAYVRAVAGRPDGVA
                     PPAGDRGP"
     gene            complement(2845..>3108)
                     /locus_tag="PLP50_15030"
     CDS             complement(2845..>3108)
                     /locus_tag="PLP50_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA52906.1"
                     /translation="LPSFDEDAYAAAAGHDDCPLDELLDEFVGLRRSHVLMFRHLDGA
                     AWDRLGLVGGHPNAARAWAFVMAGHLRHHANVLRERYGVAVEA"
     assembly_gap    3109..3208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3209..3633)
                     /locus_tag="PLP50_15035"
     CDS             complement(<3209..3633)
                     /locus_tag="PLP50_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPA52907.1"
                     /translation="MHPDLASTLADSRSATTRWLALVEPLSSEQANWRPAPDRWSIAQ
                     VLDHLAATTGPLAPALLEAGVKARAAGRVSDGPFRYGPLSRWFLRSLAPAGARPLPAP
                     KLYRPSASALDLAETTRRFLEAQRAFESAAESAEGLDLAR"
     gene            complement(3690..4691)
                     /locus_tag="PLP50_15040"
     CDS             complement(3690..4691)
                     /locus_tag="PLP50_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52908.1"
                     /translation="MRATLRSLVAVLLEPSAPPDALARAAASLHGLLLRTSGLEDDPA
                     AHRESTLPSGRALSPFDAGRCALDPVRTAVLLRGVRAAIAAAREHARGRRIEVLYAGC
                     GPFAPLVLPLLVLEEAGDLRVTFLDVHVAAIAALRRVVERLEVGDAVADALVCDAAAD
                     APVRGTAVDVVVVEAMQRSLGHEPQVAIVRNLVPLLAPHGVLVPERIRIDLVLSDPDV
                     EIADGFASARAEAARVPVGTVFELSRWSASAPLDGDGFLPPVVVTLPVPIGPRARPML
                     FTRVDAFGAHTLATAASGLTVPEPLWRVPDLPAGSRLAFRYRLGSDPRLAWSSPAPP"
     gene            complement(4769..5806)
                     /locus_tag="PLP50_15045"
     CDS             complement(4769..5806)
                     /locus_tag="PLP50_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPA52909.1"
                     /translation="MITFFTPLHEGHAPRYEYFRGERVPCFESPVRARYVHDALVAAG
                     HELLTPTVDSAPLLARVHSPRYLAFLESAWSQWLARDPANAAVQPFPSVWPVRTLRSD
                     VEPDDFVARLGLYSMDNGTPLAAGTWAAVKAAADAAASAAEAVAAGARCAFSATRPPG
                     HHAGADFMGGYCFLNNAAVAAEALLLRGSARVALLDVDYHHGNGTQSLFYGRSDVFFA
                     SLHADPRFEYPFYLGHADETGEGAGEGFNLNLPLPAGTRGPEWLAALETACARAAAAG
                     ADAIVVSLGLDTFAGDPLTKFALERDDFRRLGERLARLGLPTVLVLEGGYAAAELGTN
                     AAGVLEGFEGA"
     gene            complement(5878..6801)
                     /locus_tag="PLP50_15050"
     CDS             complement(5878..6801)
                     /locus_tag="PLP50_15050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPA52910.1"
                     /translation="MNILDAIGNTSLVRLRRLVPAGSADVLVKLEGENPTGSMKDRMA
                     LAVISRAEADGRLRPGGTVVEYTGGSTGTSLAFVAAAKGYRIRIVTSDAFSREKRDHM
                     AALGAELTLVPSEGGLTTKKLILDMIAAAREIAREPGTFFTNQLENADAIAGYVPLAE
                     ELLEEAGGKVDAFVHSVGTAASLRGIAAVLKGRRPGVRIVAVEPAESSVLLGGPAGPH
                     KIEGVGIGYTPPLWEPSLVDEVLAVATEEAKEMTRRLAREEALFAGTSTGANVVAALR
                     VAARLGAGATVATLACDTGLKYLSTDVYRRS"
     gene            complement(6881..7333)
                     /locus_tag="PLP50_15055"
     CDS             complement(6881..7333)
                     /locus_tag="PLP50_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPA52911.1"
                     /translation="MKKIRHSITIRAPREAVWAAMLGPETYRVWTTPFCEGSYYEGSW
                     NTGDRIRFLGPGGEGGMTSVIAESRPPEFVSIKHLGIVKDGVEDTTSPEATAWAPSFE
                     NYTFVDVGGATEVTAELETPPDFEAFMNEAWPKALAKLKEICEAAAPL"
BASE COUNT         1036 a         2508 c         2910 g          906 t
ORIGIN      
        1 caccacaacc acgtcgccat ggagctcctc cacaccgcgt ggaaggcccg cgactcgctc
       61 accgactccg acgccctcgt cgggatctcc ctcctctcga gccgtcccgc tggcctgcgg
      121 cggctccagg aagcggtcgg acgggacgcc ggaacgacgg agaacggaga gaacgaatga
      181 ctcgaagggt aagacggctc ctcctgaccc ccgccctcct cctcgctccg ctcttcggcg
      241 cgggacggct cgaggcgcag ccggtagccc cggccgcgcg cccggccgcg gcggacaccg
      301 cggctctcgt cgaaaggcac gtggcctggc tcggaggctg gccggcgctc gacgcgatga
      361 aagacctcag cctcgacggg acgatccgcg tcgccggact ctccggcagc ctcgccgtgc
      421 gggcgcggcg cgacggccgc cagagaacgg aggtcgacct gaaggtcctg cgcagcgtcg
      481 aagtcctcgc gggaagcgag gggtgggagc gaaacgcaag cggccagatc gagccgatgg
      541 ggctcgacaa ggtccggctc cagcgacgcg cgctcgacag gacgttcggc cggcacctgc
      601 gcggagaggg agtcgtcacg agcctcgccg gcaccgaggc gaggggaggc cgctcctggt
      661 cggtcctccg cttcacctac cccgacggcg acgcctacga cctcctcgtc gacccggaga
      721 caggggagtc gcggtacgag agaagcgtcc aggacggccg gacgacgtgg acgtcctccg
      781 cggacgtccg ggtcgtgaaa gggattcgcg tcgccgcccg acaggagatc gaaggggaga
      841 cggccctcca ggcgcagagc ctggcctggg agagcatcgc cgtgaatggc gggctcgccg
      901 acgccctctt cgtgccgccg gccccggccg gttcgctcgt tcgtctcccg gcggggcgga
      961 cgacgaccga atgggtcccg gtggacctct atcggaagcg gtacgtcttc ctcgcgggag
     1021 agctgaacgg tgtcccgacc gacgtcgtcc tcgattccgg tgccgggatg accgtcctcg
     1081 accgggcgct ggccgagaag ctgaagctcc gtcccgaggg cgagctcgag gcgcgcggta
     1141 ccggagggag cgtcggcgcg ggcctcgtca gcgggctcac gctgaaggtg gcgggagtcg
     1201 aggtcgggcc gatctcggcg gcggtgatcg acctctcgga cgtcgggcga aggctcggcc
     1261 ggccgctccc cgtcatcctc ggcaaggagc tcttccacgc tctcgtcgtc gacctggact
     1321 accccgggtt gcgcctgcgg ctccacgacg cggcgacgtt ccggtacgag ggacccggcc
     1381 ggaaggtcga cctcctcccg gccgaggatg ggcacaagag cctccggatc tcgatcgagg
     1441 gagacgagcc ggtcgtcgtc gggctcgaca cggggcaggg gggcgcgctc tcggtcttcc
     1501 ggcactacgc cgacgcgcgg aggctcctcg aggggcgccc ggtctccgag aggcgaggcg
     1561 gcggcgtcgg cggcgccacc ctctcgaagc tcgcgacgct gcggtcggtc tccgtggcgg
     1621 gctacgacct gcggaacgtg ccggcgacct tccagacgac ggacgtcggc ggcgccttcg
     1681 acacgaagcg gcaggagggg aatctcggcg cgggaatcct cgcccgcttc cgggtcctct
     1741 tcgactacgc gcgctccgct ctctggctcg agccgggacc aggtctcgcc gcgccgttcc
     1801 cgaaggacaa gaccggcctc gacctggact gggccgacgg ggctctcgtc gtcgagcacg
     1861 tcgcgccggg aagccccgcc gcggcgacag ggtggaagga aggcgagcgc gtgacggcgc
     1921 tcgacggaga gccggcgagc ccggattcgt ggcgaacgga gctccgctgg gccgaggccg
     1981 aagcggggac gaaggtgcgc ctcaccctcg cggacgggac ggagaggacg ctcgtcctgg
     2041 ccgagtacta ctgacggacg ctcaggggcc ccggtccccg gccggcggag cgacgccgtc
     2101 gggccgcccc gccacggcgc gaacgtaggc ctcggcgagc tcggcctcgg tccgggccga
     2161 gacgccaagg tcgcggagga ggccgtcctc cagggcgtag acccagccgt ggagcgagag
     2221 ctcctggccg cgcgaccagg cgtcgcggac gacggtcgtc tgggcgacgt tccggacctg
     2281 ctcgacgacg ttcagctcgc agagccggtc gcggacggcg cgctccgggc ccagccggcc
     2341 gagctgcgcg tcgtgcttct cccggacgtc ggcgacgtgg cggagccagt tgtcgacgag
     2401 gccgtgccgc tctccccgga gcgccgcgag gacgccgccg cagccgtagt gcccgcagac
     2461 gacgacgtgt ttcacgcgca ggacgtcgac ggcgaactgg agggcggaga ggccgttcag
     2521 gtcgctctgg acgacgaggt tcgcgacgtt tcggtggacg aagagctcgc ccggcaggag
     2581 cccgacgatc tcgttggccg ggacgcggct gtcggagcag ccgatccaga agtagggagg
     2641 cgcctgctgc cggcagaggc gcgtgaagaa ctccgggtcg ttccggcgga ggccgtcggc
     2701 ccaggcgcgg ttgttctcga agaggttcgg caggtgcttc atgcgttcca cggcctccgg
     2761 gtccgggcgg gtgcggtcgc ggggtggccc tcggagacga cggtcatcgc ggggcgccga
     2821 cgcgaacggg ctcctcgcgg tcgttcacgc ctcgaccgcg acgccgtagc gctcgcgaag
     2881 gacgttcgcg tggtggcgga ggtgccccgc catgacgaac gcccaggcgc gggcggcgtt
     2941 cgggtgccct ccgacgaggc cgaggcggtc ccaggcggcg ccgtccaggt ggcgaaacat
     3001 caggacgtgg ctccgacgga ggccgacgaa ctcgtcgagg agctcgtcca gggggcagtc
     3061 gtcgtgcccg gcggcggcgg cgtaggcgtc ctcgtcgaag ctcggcagnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggcgaggtcg agcccttcgg ccgactccgc
     3241 cgcgctctcg aacgcgcgct gggcctcgag gaaacgccgc gtcgtctcgg cgaggtcgag
     3301 cgccgaggcc gagggccgat agagcttcgg cgcggggagg ggccgggcgc cggcgggggc
     3361 cagcgaccga aggaaccagc gggagagcgg accgtaccgg aacgggccgt cggagacgcg
     3421 gcccgcggcg cgcgccttca cgccggcctc gaggagggcg ggggcgaggg gaccggtcgt
     3481 cgcggcgagg tggtcgagca cctgcgcgat cgaccagcgg tcgggagcgg ggcgccagtt
     3541 cgcctgctcg gaggagagag gctcgacgag cgcgagccac cgggtcgtcg cggagcggga
     3601 gtccgcgagc gtcgaggcga ggtcggggtg cacggcggga gcgtagcgcc ccggatgcgc
     3661 cggggtccac gggcggtgcg cggccgggct cagggcgggg ccggcgacga ccacgcgagg
     3721 cggggatcgc ttccgagccg gtagcggaac gcgagacggc tcccggcagg gaggtcggga
     3781 acccgccaga gaggctcggg aacggtaaga cccgaggcgg ctgtcgcgag cgtgtgagct
     3841 ccgaaggcgt cgacccgtgt gaagagcatc ggccgggcgc gggggccgat cgggacgggc
     3901 agggtgacga cgacgggagg gaggaacccg tcgccgtcga gaggcgcgga ggcgctccat
     3961 ctcgagagct cgaagaccgt cccgacgggg acgcgcgccg cctccgcgcg ggccgaggcg
     4021 aagccgtcgg cgatctcgac gtcggggtcc gaaaggacga ggtcgatgcg gatccgttcc
     4081 ggcacgagga cgccgtgggg ggcgaggagc ggaacgaggt tccggacgat cgcgacctgc
     4141 ggctcgtgcc cgaggctccg ctgcatggcc tccacgacga cgacgtcgac ggcggtcccg
     4201 cggaccggcg cgtcggccgc cgcgtcgcag acgagggcgt ccgcgacggc gtcgccgacc
     4261 tcgagacgct ccacgacgcg acggagtgcg gcgatggccg caacgtggac gtcgaggaac
     4321 gtgacccgaa ggtccccggc ctcttcgagg acgaggagag ggaggacgag cggcgcgaac
     4381 ggaccgcacc cggcgtagag gacctcgatc cgccgcccgc gggcgtgctc ccgcgccgcc
     4441 gcgatcgcgg cgcggacacc ccggaggagg acagcggtcc ggaccgggtc gagcgcgcag
     4501 cgcccggcgt cgaacgggga gagcgcacgc cccgaaggaa gcgtcgattc gcgatgggcc
     4561 gccggatcgt cctccagccc cgacgtgcgg aggaggagtc cgtggagcga cgccgccgct
     4621 cgggcaagcg cgtcgggcgg ggcggacggc tcgaggagga cggccacgag ggagcgaagg
     4681 gtggcccgca cggcgcgaat cacggcgggc cgccggaggc cgtgcggcgg acgcacgcaa
     4741 ggagagggga tcgcacggcc ggcgtccgtc aggcgccctc gaacccttcg aggacgcccg
     4801 ccgcgttcgt gccgagctcg gcggccgcgt agcccccttc gaggacgagg acggtcggga
     4861 ggccgaggcg tgcgagccgc tctccgaggc ggcggaagtc gtcccgttcg agcgcgaact
     4921 tcgtcagcgg gtctccggcg aacgtgtcga ggccgagcga gacgacgatg gcatccgcgc
     4981 cggccgccgc cgcccgggcg caggccgtct ccagggcggc gagccactcc ggcccgcgcg
     5041 tgcccgccgg gagcgggagg ttcaggttga agccctctcc ggcgccctcg cccgtctcgt
     5101 cggcgtggcc gaggtagaac gggtactcga agcgcgggtc ggcgtggagg ctcgcgaaaa
     5161 agacgtcgga ccggccgtag aagaggctct gcgtcccgtt gccgtggtgg tagtcgacgt
     5221 cgaggagggc gacgcgcgcg ctgccgcgga ggagcagcgc ctcggcggcg acggcggcat
     5281 tgttcaggaa gcagtagccc cccatgaagt cggcgcccgc gtggtgcccg ggggggcgcg
     5341 tcgcggagaa cgcgcagcgc gcccccgcgg cgacggcctc ggcggcgctc gcggcggcgt
     5401 cggccgcggc cttcacggca gcccaggtcc cggccgcgag cggcgtcccg ttgtccatcg
     5461 agtagaggcc gaggcgcgcg acgaaatcgt cgggctcgac gtcgctccga agcgtccgga
     5521 ccggccagac ggacgggaac ggctggacgg cggcgttcgc cgggtcccgt gcgagccact
     5581 ggctccaggc ggactcgagg aacgcgaggt accgcggcga gtggacgcgg gcgaggaggg
     5641 gggcggagtc gaccgtcggc gtgaggagct cgtgcccggc ggcgacgagc gcgtcgtgga
     5701 cgtagcgggc gcggaccggc gactcgaagc aggggacccg ctcgccgcgg aagtactcgt
     5761 accggggcgc gtgtccttcg tgaagcggcg tgaagaaggt gatcacgcgc cgatcgtagc
     5821 ggctcggcgg cgggcgtggg aggcccggag gcttcgcgag ccggcgccgg cggcgcctca
     5881 ggagcgacgg tagacgtcgg tgctgaggta cttgagcccg gtgtcgcagg cgagggtggc
     5941 gacggtcgcg cccgccccga gacgcgcggc gacgcggagc gcggcgacga cgttcgcgcc
     6001 ggtggaggtg ccggcgaaga gcgcctcctc gcgcgcgagc cgccgcgtca tctccttcgc
     6061 ctcctccgtc gcgacggcga ggacctcgtc gacgagggag ggctcccaga gcggcggcgt
     6121 gtagccgatg ccgaccccct cgatcttgtg cgggccggcc gggccgccga ggaggacgga
     6181 cgactccgcc ggctcgacgg cgacgattcg cacgccgggg cgtcggccct tgaggacggc
     6241 cgcgatgccg cgcagcgagg ccgccgtccc gacggagtgg acgaaggcgt cgaccttccc
     6301 gcccgcctcc tcgaggagct cctcggcgag cgggacgtac ccggcgatcg cgtcggcgtt
     6361 ctcgagctgg ttcgtgaaga aagtccccgg ctcccgggcg atctcgcggg cggccgcgat
     6421 catgtcgagg atcagcttct tcgtcgtcag gcccccctcg ctcgggacga gcgtcagctc
     6481 ggcgccgagc gcggccatgt gatcgcgctt ctcgcggctg aaggcgtcgg aggtgacgat
     6541 ccggatccgg taccccttcg cggcggccac gaacgcgagc gaggtgccgg tgctcccgcc
     6601 cgtgtactcg acgacggtgc cgccgggacg caggcgcccg tcggcctcgg cgcgcgagat
     6661 cacggcgagc gccatccggt ccttcatgct cccggtcggg ttctccccct cgagcttgac
     6721 gaggacgtcc gcggaacccg cggggacgag cctgcggagg cggacgaggg acgtgtttcc
     6781 gatggcgtcg aggatgttca tgcgcgggac ctgcctccgc gcgaggatag ccccgcgcgg
     6841 cgcgtgtcgc gtgtcgaggg gacggcggcc ggccggcggc tcagagggga gcggccgctt
     6901 cgcagatctc cttcagcttc gcgagcgcct tcggccaggc ttcgttcatg aacgcctcga
     6961 agtcgggagg cgtctccagc tcggcggtca cctccgtcgc gccgccgacg tcgacgaacg
     7021 tgtagttctc gaacgagggg gcccaggccg tggcctcggg gctcgtcgtg tcctcgacgc
     7081 cgtccttgac gatcccgagg tgcttgatcg agacgaactc cggcgggcga ctctcggcga
     7141 tgacggacgt catccccccc tcgccgcccg gcccgaggaa gcggattcgg tccccggtgt
     7201 tccaagagcc ctcgtagtac gagccctcgc agaagggcgt cgtccagacc cggtaggtct
     7261 cgggtccgag cattgcggcc caaacggcct cgcgcggcgc ccggatggtg atcgagtgac
     7321 ggatcttctt catgggcctc tcctttcgga cgattcgggc gacgtccggc cgacgggcca
     7381 ctcggtcggc cgcgggacgc tccgaattcc gggatggttt gtggctctct ccgacggtgg
     7441 cggggcggat ggattccggg
//
LOCUS       DAOONL010000149         7400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_11863_length_7400_cov_20.812253, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000149 DAOONL010000000
VERSION     DAOONL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11863_length_7400_cov_20.812253"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLP50_15060"
     CDS             complement(<1..436)
                     /locus_tag="PLP50_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPA52912.1"
                     /translation="MSVAPPLPRTGEELREAIRWHVRHSLGKAWEDATNGDLFQALGL
                     AVRDRAWERLLATEKRYRQAGAKRVYYLSMEFLIGRSLVDSLHNLGLHDAAIRALAEL
                     GHDLDHVREEEVDAGLGNGGLGRLAACFLDSLATLHLPGYGYG"
     gene            complement(433..774)
                     /locus_tag="PLP50_15065"
     CDS             complement(433..774)
                     /locus_tag="PLP50_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPA52913.1"
                     /translation="MNLTIEPRESHILARIEDARLDALSAPDFKARMSGALTGTAPKV
                     VLDLSPVAFIDSAGLASILSLVKGLPEGGDLRIAGARDAVRAVFRLTRLDKVLALHDT
                     VEDAVAAAEAR"
     gene            997..2382
                     /locus_tag="PLP50_15070"
     CDS             997..2382
                     /locus_tag="PLP50_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPA52914.1"
                     /translation="MPAALLLAAVLSAVPPAPAPEALHAAWPGVRLSPPSGSYTPEAL
                     RADLEALAGASRGVVRIAEKGRSSEGREIPVVLAGSGPEKVLLWSQMHGDEPTATAAL
                     VDLLAFLAENRAEPATAHLLSRLTLVVVPMLNPDGAVRGDRRNAQGIDVNRDATRLQT
                     PEGRFIKEIRDRYTPAAGFNLHNQSPLVTAGPGGAQAAIAVLAVSAARNEPEGPALQR
                     KRGLAGHLARVASTFVPGRVTRYATDYTERAFGDSMSRWGTPTVLLETGGWHGPREEE
                     RLVRLNFVLLLSALHALARGEETADAAIYETLPPNVRGDLVDLVLRGATVHGGRGLPP
                     FRADVAVVKARAFAGDGRRLPSAAVVDVGDLGHLAGLEEVDATGLVVAPAPAGGAEAW
                     EKVVAALAARGLAKDGALLLDDDALAREARAWAAGRAIVPWAEVDLVLFTRAEKGLVV
                     ESFARGGTRTK"
     gene            2492..4591
                     /locus_tag="PLP50_15075"
     CDS             2492..4591
                     /locus_tag="PLP50_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52915.1"
                     /translation="MNEIVARRAASALLCLLWLLHADVAVAESFREKVARWDALAVEA
                     ASRPADDLVLTSGRLTLELKKGRVAEVRAGEEVVGLFVSGTGTLRYRSDDPSERDAVR
                     WATRKNTGLRLSEEEGALVLSTRFTDALWVSAGTPLPGIPAPAAEASLASSFAKHRAR
                     FDNLAARPLLHQLLHARGSGDAAPVVRVEVNAAGDDLVYLLDGLVSRSERLSAAHVIP
                     ALRTAGDDRKLLAVLSDTPLGRDRREPAPPEVFLTHVDLSVAASGGSLATVEATETFE
                     AASGKPGVLLLHQKGTFFRPRNTGNLLKQEARVTAVRDAAGGALEFDHRDDFLAVALP
                     SPLVPGRPVTLRFEIEGDLLHRPFGASYWLLGNEAWFPRPGERERAFTVRSRVRVKRP
                     FTPLAPGTTVRRAEEGDEQVLETRIDKPVTAFSVLAGRFSLEEEARDGLTVRAATYAG
                     PNTISQRNILEAARKMTVHLAGYLGNAPFDEITVVQLDRAAPDPVLSGFVFLPTRFDF
                     ASIHPEPLPAGFDYPSGGTPRWFVMKNGVSRRLARGLAEQWWGASVRSAGGGESWVPK
                     AFSDYLAGLLVRDLHSEIDWTLLRSMWKKDARASRRVAPVALWDRVFAFDDVGRSIQL
                     QDSLVAARGPLLLDGLREELGEERFRTLLAAVQKTFAGRRASTGDVARVLAAVSSRET
                     ANAFESRVWERAADWAR"
     gene            4680..6734
                     /locus_tag="PLP50_15080"
     CDS             4680..6734
                     /locus_tag="PLP50_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPA52916.1"
                     /translation="MPNSRSSYARAALLLAVLFVVAPVTAAETFPETIRRYDTLAVDA
                     SSRDATGMVLRSGRMVLRVADGRVAPVRAGTEVVGLFVAGKGTLEYVSTDAVEHPVVK
                     YTAKKNSGLDVTQAEGGLVLTEAFSSALWLASGAPLPALPETADAGSLADDFGKHRER
                     FGNVAQRPVAMDLARARHDAPAQPWVRVEATGSADDLVYLLDGVTDRVESLSVASRFQ
                     YTAASGDSRKFVTVLSEQPVGRDRRQPVPPPYLLTDVDLSLTASDGESAALTVTETFQ
                     AVAAQPGVLFLDLYSAINRRDNHGNVVPKTVRLKGVFDEGGRPVEHVHRDSHLAVRLR
                     EPLARGRGTKLRFEIEGDLLYRPGGDNYWELGTEPWFPQPELNCQFYTVHATVKVKKP
                     FVPLVPGTTVRRIEEGDYNLLETRIAKPVQLFVVIAGKYRFEEETKDGLTIRVASYGG
                     PNRMAYRKLLELSRKMIDYYSGFLGAFPFDEFNIVEINAYGYGQAPPAFMFITTEAFN
                     PIQGEVNQIFSQGINERFAHEIAHQWWGHVVKMPSADEQWISESFAEYSAALLIRDLR
                     GKSDYKGLLAHWRRRANEARDVAPVALATRVRARNDLETAWNLRTALLYSKGPLVLEA
                     LHRELGDRPFLVFLASVQSNLTWRFGPTGLVQQVLNAVAKKDYGPFLEKYYWGLEMPP
                     AK"
     gene            complement(6831..7187)
                     /locus_tag="PLP50_15085"
     CDS             complement(6831..7187)
                     /locus_tag="PLP50_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HPA52917.1"
                     /translation="MPTFIEKPSVVVAAGNLPKRIEELVGRVNTATAGLSIARMTSPA
                     GWVEPGQTPEFDEYSVVLSGTLRVESREGALDVRSGQAVVARAGEWVRYSTPEGAEYV
                     SVCVPAFSPGTVHRDA"
     gene            complement(7209..>7400)
                     /locus_tag="PLP50_15090"
     CDS             complement(7209..>7400)
                     /locus_tag="PLP50_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HPA52918.1"
                     /translation="APFSWEPALVEVLDSGTLALSTGPVRDPAGKETGTFTSIWRREG
                     PGTWRIVFDKGNPVCPKEP"
BASE COUNT         1019 a         2763 c         2585 g         1033 t
ORIGIN      
        1 ggccgtagcc gtagcccggc aggtggagcg tcgcgagcga gtcgaggaag caggccgcga
       61 ggcggccgag gccgccgttg ccgaggccgg cgtcgacctc ctcctcgcgc acgtggtcga
      121 ggtcgtgccc cagctcggcg agcgcccgga tggccgcgtc gtggaggccg aggttgtgga
      181 gcgagtcgac gagcgagcgc ccgatgagga actccatcga gaggtagtag acccgcttgg
      241 cgccggcctg ccggtagcgc ttctccgtcg cgaggaggcg ttcccaggcc cggtcgcgca
      301 cggcgagccc gagcgcctgg aagaggtcgc cgttcgtggc gtcctcccag gccttcccga
      361 gcgagtgtcg gacgtgccag cgaatcgcct cgcgcagctc ctccccggtc cgcggcagcg
      421 gcggggcgac gctcaccggg cctccgcggc ggcgaccgcg tcctccaccg tgtcgtggag
      481 ggcgagcacc ttgtcgagcc gcgtcaggcg gaagacggcg cggacggcgt cacgcgcgcc
      541 ggcgatccgg agatcgcccc cctcggggag ccccttcacg agcgagagga tggaggcgag
      601 cccggcgctg tcgatgaacg cgacggggga aaggtcgagg acgaccttcg gcgccgtccc
      661 ggtgagcgcg cccgacatgc gcgccttgaa gtcgggggcg gaaagggcgt cgaggcgggc
      721 gtcctcgatc cgggccagga tgtgagactc cctcggctcg atcgtcaggt tcatcgttcc
      781 tccctccgct ccgtccggaa acgcgacgac cggtcgcccc gcggtgagcc ccctcgatcc
      841 gtgggccctt ccggcacgcc gcgggaggcg cctccgggga agctcgttcc cgttcgtttc
      901 ggacgcgggc agcttgccac cggcgtcgga ggtgtcaagg cgggccgcca gcgggcaaac
      961 ggtgcccgag ggccggatcg ctggtaccgt tcgaccatgc cggccgcgct cctcctcgcc
     1021 gccgtcctct cggccgttcc tcccgccccc gccccggagg cgctccacgc cgcctggccg
     1081 ggcgtgcggc tctcgccccc gtccggctcg tacacccccg aggcgctccg ggccgacctc
     1141 gaggcgctcg ccggcgcctc ccgaggcgtc gtccggatcg cggagaaggg gcggtcgtcc
     1201 gaggggcggg agatcccggt cgtcctcgcc gggagcggcc cggagaaggt cctcctctgg
     1261 tcgcagatgc acggcgacga gccgaccgcg acggcggccc tcgtcgacct cctcgcgttc
     1321 ctcgccgaga accgggccga gcccgccacc gcgcacctcc tttcgcgcct caccctcgtc
     1381 gtcgtcccga tgctcaaccc cgacggcgcc gtgcgcggcg accggcgaaa cgcgcaggga
     1441 atcgacgtca atcgcgacgc cacgaggctc cagacgccgg agggacgctt catcaaggaa
     1501 atccgcgacc gctacacccc ggcggccggc ttcaacctcc acaaccagag ccccctcgtg
     1561 accgccggac cgggaggagc gcaggccgcg atcgcggtcc tcgccgtttc ggccgcccgg
     1621 aacgaacccg aggggccggc cctccagcgc aagcggggcc tcgcggggca cctcgcccgc
     1681 gtcgcctcga cgttcgtccc gggccgcgtc acccgctacg ccaccgacta caccgagcgc
     1741 gccttcggcg actcgatgtc gcgctggggg accccgaccg tcctcctcga aaccggcggc
     1801 tggcacggcc cgcgggagga ggagcgcctc gtccggctca acttcgtcct cctcctgtcc
     1861 gccctccacg ccctcgcgcg cggcgaggag actgcggacg cggccatcta cgagacgctc
     1921 ccgccgaacg tccgcggcga cctcgtcgac ctcgtcctgc gcggcgccac cgtccacggc
     1981 gggcggggcc ttcctccctt ccgcgccgac gtcgcggtcg tgaaagcacg cgcgtttgcg
     2041 ggcgacggtc ggcgtctccc ctcggccgcc gtcgtcgacg tcggcgacct cgggcacctc
     2101 gccggcctcg aggaggtgga cgccaccggc ctcgtcgtcg cgcccgcgcc ggccggaggc
     2161 gcggaggcgt gggagaaggt cgtcgccgct ctcgccgcac ggggcctcgc gaaggacggg
     2221 gcgctcctcc tcgacgacga cgcgctcgcg cgcgaagccc gcgcctgggc cgcgggtcgg
     2281 gcgatcgtcc cctgggcgga ggtcgacctc gtcctcttca cgagagccga gaaggggctc
     2341 gtcgtcgaaa gcttcgcccg cggggggacg cggacgaagt aggccgggtg tccctccgcg
     2401 gtgacggacg tcacggtacg ccgattgcat cgtggcgaga cacgaaggat tccgtccgac
     2461 cgttcgagcc gggcggtacc gagaggtggt tatgaacgag atcgtcgccc ggcgtgcggc
     2521 ttcggcgctc ctctgcctcc tgtggctcct ccacgcggac gtcgccgtcg ccgagagctt
     2581 ccgcgagaaa gtcgcccgct gggacgccct cgccgtcgaa gcggccagcc gccccgccga
     2641 cgacctcgtc ctgacgtcgg gacggctcac gctggagctg aagaaagggc gcgtcgccga
     2701 ggtccgggcc ggcgaagagg tcgtcggtct cttcgtctcg ggaaccggga cgctccggta
     2761 ccgctccgac gacccgtccg agcgcgacgc ggtgcgctgg gccacccgga agaacacggg
     2821 cctgcggctt tcggaagagg agggggccct cgtcctctcc acgcggttca ccgatgccct
     2881 ctgggtctcc gccggcacgc cgctcccggg gataccggcg ccggccgccg aggcctcgct
     2941 cgcgtcctcc ttcgcgaagc accgggcccg cttcgacaac ctcgccgcgc ggcccctcct
     3001 ccaccagctc ctccacgccc ggggctccgg agacgccgcg ccggtcgtcc gcgtcgaggt
     3061 gaacgcggcg ggcgacgacc tcgtctacct cctcgacggc ctcgtctcgc gctccgaacg
     3121 gctctccgca gcgcacgtga tcccggctct ccggacagcg ggcgacgatc ggaagctcct
     3181 cgccgtcctg agcgacacgc ccctcgggcg ggaccggagg gaaccggctc cgccggaggt
     3241 tttcctgacg cacgtcgacc tctcggtcgc ggcctcgggc ggctccctcg ccacggtgga
     3301 ggcgaccgag acgtttgaag ccgcctccgg gaaaccgggc gtcctcctcc tccaccagaa
     3361 gggaaccttc tttcgcccgc ggaacaccgg gaacctcctg aagcaggaag cgcgtgtgac
     3421 ggccgtccgg gacgcggcgg gaggggcgct ggagttcgac caccgggacg acttcctcgc
     3481 cgtcgcgctg ccgtccccgc tcgtcccggg ccgtcccgtg acgctccgct tcgagatcga
     3541 gggcgacctc ctccatcgcc ccttcggcgc ctcctactgg ctcctcggaa acgaggcctg
     3601 gttcccgcgc cccggcgagc gggagcgcgc gttcacggtc cgctcccgcg tgagggtgaa
     3661 gcggcccttc acgcccctcg ccccggggac gaccgtgcgg agagccgagg agggggacga
     3721 gcaggtcctc gagactcgga tcgacaagcc cgtgacggcc ttctccgtcc tcgccggcag
     3781 gttctccctc gaggaggagg cgagggacgg cctcacggtt cgggccgcga cctacgccgg
     3841 cccgaacacg atctcgcagc gaaacatcct cgaggccgcg cggaagatga ccgttcacct
     3901 cgccggctac ctggggaacg ccccgttcga cgagatcacc gtcgtccagc tggaccgggc
     3961 ggctcccgac ccggtcctct cgggtttcgt ctttctgccg acccgcttcg acttcgcttc
     4021 gatccacccc gagccgcttc ccgcggggtt cgactacccg agcgggggga ccccgaggtg
     4081 gttcgtgatg aagaacggcg tcagccgacg cctcgcccgg ggtctcgcgg agcagtggtg
     4141 gggcgcctcg gtccggagcg cgggcggcgg ggagagctgg gtgcccaaag cgttctcgga
     4201 ctacctggcc gggctcctcg tccgcgacct ccacagcgag atcgactgga ccctcctgcg
     4261 atcgatgtgg aagaaggacg ctcgggcctc gcgcagagtc gcacccgtcg ccctctggga
     4321 ccgcgtcttc gcgttcgacg acgtcggccg gtcgatccag ctgcaggaca gcctcgtggc
     4381 cgcgcgcggc ccgctcctcc tcgacggatt gcgcgaggag ctcggcgagg aacgcttccg
     4441 gacgctgctc gccgcggtgc agaagacgtt cgccggccgg cgggcctcga cgggcgacgt
     4501 ggcgcgggtc ctcgccgcgg tctcgtcgcg ggagacggcg aacgccttcg agtcgcgcgt
     4561 ctgggagcga gccgcggact gggcccgcta ggcggcggtg cgcggggcgt atcggtccga
     4621 tctcgggcag aatctgcggt cggcctcgac aacgccacat cgcaaccgga ggaaacccag
     4681 tgccgaattc ccgttccagc tacgcccgcg cggctcttct cctcgccgtg ctcttcgtcg
     4741 tcgcccccgt caccgccgcg gagacgttcc cggagacgat ccgccgctac gacaccctcg
     4801 ccgtcgacgc ctcgagccgg gacgcgacgg ggatggtcct gaggtcgggc cggatggtcc
     4861 tgcgcgtcgc ggacggccgg gtcgcgcccg tacgagcggg gaccgaggtc gtcggcctct
     4921 tcgtcgcggg caaaggaacg ctcgagtacg tctcgaccga cgcggtcgag caccccgtgg
     4981 tcaagtacac cgcgaagaag aacagcggcc tcgacgtgac ccaggccgag ggcggcctcg
     5041 tcctcaccga ggccttctcc tcggcgctct ggctcgcctc gggggcgccg ctcccggcgc
     5101 tccccgagac ggccgacgct ggctcgctcg ccgacgattt cggcaagcac cgcgagcggt
     5161 tcgggaacgt ggcccagagg ccggtcgcga tggacctcgc ccgtgcccgg cacgacgctc
     5221 ccgcccagcc ctgggtccgc gtggaggcga ccggctcggc ggacgacctc gtctacctcc
     5281 tcgacggcgt gacggaccga gtcgagtccc tctccgtcgc gagccgtttc cagtacaccg
     5341 cggcatccgg ggactcgcgg aagttcgtga cggtcctttc cgagcagccg gtcggccgcg
     5401 accggcgcca gcccgttccc ccgccctacc tcctgaccga cgtcgacctc tccctcaccg
     5461 cctccgacgg cgagagcgcg gcgctgaccg tgacggagac gttccaggcg gtcgccgccc
     5521 agccgggggt tctcttcctc gacctctaca gcgccatcaa ccgccgcgac aaccacggga
     5581 acgtcgtgcc gaagacggtg cggctgaaag gcgtcttcga cgagggggga agacccgtcg
     5641 agcacgtgca ccgcgacagc cacctcgcgg tccggctccg cgagcccctc gcgcggggac
     5701 gcgggacgaa gctgcggttc gagatcgagg gcgacctcct ctaccggccc ggcggggaca
     5761 actactggga gctggggaca gagccgtggt tcccgcaacc cgagctcaac tgccagttct
     5821 acaccgtcca cgcgacggtg aaggtgaaga agcccttcgt cccgctcgtc cccggaacga
     5881 ccgtccgccg tatcgaggag ggggactaca acctcctcga gacccgcatc gccaagcccg
     5941 tccagctctt cgtcgtcatc gccggcaagt atcggttcga ggaggagacg aaggacggcc
     6001 tcacgatccg ggtcgcctcc tacggcgggc cgaaccggat ggcgtaccgg aagctgctcg
     6061 agctctcgcg gaagatgatc gactactact cgggcttcct cggggcattc ccgttcgacg
     6121 agttcaacat cgtcgagatc aacgcctacg ggtacgggca ggcgccgccg gccttcatgt
     6181 tcatcacgac ggaggcgttc aacccgatcc agggcgaggt caaccagatc ttctcgcagg
     6241 ggatcaacga gcggttcgcc cacgagatcg cgcaccagtg gtggggccac gtcgtgaaga
     6301 tgccgagcgc ggacgagcag tggatctcgg agtcgttcgc ggagtactcg gcggcgctcc
     6361 tcatccgcga cctccgcggg aagagcgact acaagggcct cctcgcgcac tggcggcggc
     6421 gcgcgaacga ggcccgcgac gtggcgcccg tcgcgctcgc gacgcgcgtc cgggcacgga
     6481 acgacctcga gaccgcctgg aacctccgga cggcgctcct ctactccaag ggtccgctcg
     6541 tcctggaagc ccttcaccgc gagctcggag accggccgtt cctcgtcttc ctcgcgtcgg
     6601 tccagtcgaa cctcacctgg aggttcggac cgacgggcct cgtccagcag gtcctgaacg
     6661 ccgtcgcgaa gaaggactac ggcccgttcc tggagaagta ctactggggg ctcgagatgc
     6721 ccccggcgaa gtagggactt ccccgcgcgt tgcacggcgc gaccggcccg cggtggcgtc
     6781 ccgcgcggcg gaagcgcgat ggcaagagcc gggggcggtg cgacgggcgt tcaggcgtcg
     6841 cggtggaccg tccccggcga gaaggccggg acgcagacgg agacgtactc ggccccttcc
     6901 ggcgtcgagt agcggaccca ttccccggcg cgggcgacga cggcctgacc cgagcggacg
     6961 tcgagcgccc cctcgcgact ctccacgcgg agcgttcccg agaggacgac ggagtactcg
     7021 tcgaactcgg gcgtctggcc cggctcgacc cagcccgccg ggctcgtcat ccgggcgatg
     7081 ctgaggcccg cggtcgcggt gttcacgcgc ccgacgagct cctcgatccg cttcgggagg
     7141 ttcccggcgg cgacgacgac ggacggcttc tcgatgaacg tcggcatgcc gggaggatag
     7201 ccggcggctc agggctcctt cgggcagacc gggttcccct tgtcgaagac gatccgccag
     7261 gtcccgggtc cctctcgccg ccagatcgac gtgaacgtgc cggtctcttt cccggcgggg
     7321 tcgcgcaccg gccccgtgct cagggcgagc gtccccgagt cgaggacttc gaccagcgcg
     7381 ggctcccagg agaagggggc
//
LOCUS       DAOONL010000150         7291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12091_length_7291_cov_10.405058, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000150 DAOONL010000000
VERSION     DAOONL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_7291_cov_10.405058"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLP50_15095"
     CDS             <1..648
                     /locus_tag="PLP50_15095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52919.1"
                     /translation="GYAELLAEEPEVSASPGALEDVGKIRAAGGHLLQLIGDLLDLSK
                     VEAGRMEVVAEPFAVREVVEETVRTVRHLVEGNRNRFLVTIDDGVGEMTSDAFRLKQV
                     LFNLLSNAGKFTEAGTVTLDVLREERAGGEVLVFRVADTGIGMSPEQLEKVFTPFVQG
                     DASLTRRFGGTGLGLALSRHLARLMGGGIWVTSAEGKGTAFVLELPSGTPSPSTA"
     gene            810..1292
                     /locus_tag="PLP50_15100"
     CDS             810..1292
                     /locus_tag="PLP50_15100"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPA52920.1"
                     /translation="MKHSSRTNLVPVAAALALVLVAGPLLALDAPPGPRPGPQGPLAG
                     YLRCLAVVDLTEAQKADVKALFEAAKPELEALRATLKADREALLAALGAATPDACTVG
                     TAFLKVEADRKAIREATAELRTAVEALLTAEQKAKLEGCLKAPRPNGPAGEEDEGAER
                     "
     gene            complement(1700..1888)
                     /locus_tag="PLP50_15105"
     CDS             complement(1700..1888)
                     /locus_tag="PLP50_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52921.1"
                     /translation="MLDPKKAEVAMKRHFARVTPEEFRKRAASASHGDTEGKPAKPAA
                     PAAGGAAPAGRPRRVAHG"
     gene            complement(1953..2483)
                     /locus_tag="PLP50_15110"
     CDS             complement(1953..2483)
                     /locus_tag="PLP50_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52922.1"
                     /translation="MAPARHILATGYHGTHVGRLESILANGLRYSTNEWDWLGDGIYF
                     FQDSPLRAREWAEEHHGRSNAAVLRAVIRLEDCVDLLDIEWWPTLSSSHRQFVEREKV
                     LPKQTATSGAHRLDRAVLNYMVGVLAEDGQNVRCLRGAFVEGERLFPASALYSRSHVQ
                     IAVRDPLLIEDLTVLG"
     gene            complement(2498..4351)
                     /locus_tag="PLP50_15115"
     CDS             complement(2498..4351)
                     /locus_tag="PLP50_15115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPA52923.1"
                     /translation="MTESAKAPVAISAAKGRPMLSWVGKKPLSHVPALPAQHVETFDP
                     AGSLTKDADGWKGWPSGYPKGGLLFHGDNKEVLGHLLANGFRGKVDLVYIDPPFDSGA
                     DYVRRVALRGPKGTAKISGETYSLGEQIQYTDIWANDNYLQFMYERLLLLKELMAERS
                     ALALHCDWRKAHYLRCLLDETFGEGAFQNEVIWYFYNKMHDDRKGVLPRATNSILIFS
                     KGTPPFHDVDVPRDKAIRQLRRVKVDGRLVNLKDESGDLVYQESDSRTLDNVWPIPLI
                     PPADVKQKKEYPTQKPEALLEALLSVWSNPGGLVLDCFIGSGTTAAVAQKLGRRWIGC
                     DINKGAIQTTAKRVQSIIQEQTVEAAQADLPTLEPDGPRPAQAAFTTWRVNDYDLQIQ
                     HNEAVEIACEALGVERVRTDGFFDGTLGKALVRIIPFQHPLSPLDLEEVRRELGTRPE
                     EDRAVKVVCLGAEVGARAWVDDWNRLRKGSGAVNRIEVIELRTDERYGKLLKHEPAVA
                     RVKVSRTKKHLVVEVRDFVSPTIIERLERQAGVVRPEIEDWRWVVDCILIDTDYDGAV
                     FNVVLADMPEKKSDLVEGRYELPPPRPEATIAVKLIDMLGEEVLVTIPPKA"
     gene            complement(4348..7002)
                     /locus_tag="PLP50_15120"
     CDS             complement(4348..7002)
                     /locus_tag="PLP50_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPA52924.1"
                     /translation="MPAAVLHEALSRKVDAWRQGGHSCDPFSAIAEILEFAREPETGR
                     LRFLRPPQLLALETYWYLRLVEGTPHILDLYWKLFPRNSELLPALGLTDSRIQAIALD
                     EGANGVLSRIRDDDDFVRSFRLEALRESIRLDYPSYILALAMGAGKTMLIGAIVATEF
                     AMALEHPDGPFVQNALVFAPGKTILESLRELLEVPYERILPPRLHKRFAASVKLTFTR
                     DGEKGIPVTPGSLFNVVVTNTEKIRIRKEDVRRGDIGPLFGEGREDEARSEVANLRLQ
                     AIASLPHLAVFSDEAHHTYGQSLDADLKKVRKTVDYLAANTNVLCVVNTTGTPYFQRQ
                     PLRDVVTWYGLSQGIRDGILKDLAGSIQAYDFDGEPGPYLRHVVEDFFRDYGEVRLPN
                     GAPAKLAIYFPQTDDVVELRPIIDETLARLGLSPALCLTNTSDSRLTSEADVEAFNRL
                     NDPAAPHRVILLVNKGTEGWNCPSLFACALARKLKSSNNFVLQAATRCLRQIPGNARK
                     ARVYLSTDNFSILDRQLQETYGESIRDLDHAGVERGRARIVLRKVDIPPICVRRTLRT
                     VVPVAKARTPLRLERPAAYGDVGLEIKTFTISEQVASAGVLRQVGDAVQIDSEIDAST
                     AMTVAVRLAALHGLDLWEALDEVRRLYPEAPVPNADIPHLSAQIEKQLGQHEVKEEAV
                     DVALALVKPSGFEKEIGPDGAEVYTAEIVYPKDRERLLLARETLEDRNPGDFGFHYDP
                     YAFDSNPEKSFLELVLEELRLDPGEIEDVYFTGGLTDPSRTDFFVEYKDEKGKWRRYT
                     PDFVVRKKPARGAPAGSGRAVIVEIKKEFDRAHPTDGESGRKALAIRRWEQLDPDRLR
                     YEILFTPGDTVTVDQTRRTLAPLRKEPP"
     gene            complement(7128..>7291)
                     /locus_tag="PLP50_15125"
     CDS             complement(7128..>7291)
                     /locus_tag="PLP50_15125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPA52925.1"
                     /translation="GLPERVTSSAGVAEHARGETTEALVARADAALYEAKRQGRDRVV
                     LAPPPEPAG"
BASE COUNT         1085 a         2376 c         2653 g         1177 t
ORIGIN      
        1 ggctacgccg agctcctcgc cgaggagccg gaggtgagcg ccagcccggg cgcgctcgag
       61 gacgtcggga agatccgcgc cgccggcggc cacctcctcc agctcatcgg agacctcctc
      121 gacctctcga aggtggaagc gggacggatg gaggtcgtcg cggagccgtt cgcggtgagg
      181 gaggtcgtcg aggagaccgt ccggacggtg cgccacctcg tcgaagggaa ccggaaccgc
      241 ttcctcgtga cgatcgacga cggcgtgggc gagatgacgt ccgacgcgtt ccgcctcaag
      301 caggtcctct tcaacctcct ctcgaacgcc gggaagttca ccgaggccgg gacggtgacg
      361 ctcgacgtcc ttcgcgagga gcgagccggc ggcgaggtcc tcgtcttccg cgtcgccgac
      421 accggcatcg gcatgagccc ggagcagctc gagaaggtct tcaccccgtt cgtccagggg
      481 gacgcgtcgc tcacgcggcg cttcggcggg accggcctcg gcctcgccct ctcccggcac
      541 ctggcgcggc tcatgggggg cggcatctgg gtgacgagcg cggaagggaa gggaacggcg
      601 ttcgtcctgg agctgccgtc cggaacgccc tcgccgagca cggcgtaggg cggaacgggg
      661 cgccggggcc gcgcggcctc gtcaccgggc gggccgtcgt gtcaaccgcg agccgcgtcg
      721 ccgcgtcgat aggggtgccc accgaaagag cggcgacagg ccggcacggt ccgtcgccgc
      781 aacgtacgga aagggagaag gagcctcgta tgaagcactc ctcacggacg aacctcgtcc
      841 ccgtggccgc ggcgctcgcg ctcgtcctcg tcgcgggccc cctcctcgcg ctcgacgcgc
      901 ctcccggtcc gcgccccggg ccgcaggggc cgctcgcggg ctacctccgc tgcctcgccg
      961 tcgtcgacct gacggaggcg cagaaggcgg acgtgaaggc gctcttcgag gcggcgaagc
     1021 cggagctgga ggctcttcgg gcgacgctga aggccgatcg ggaggcgctc ctcgccgcgc
     1081 tcggcgccgc gacgccggac gcctgcaccg tcgggacggc gttcctgaag gtggaggccg
     1141 accggaaggc gatccgcgag gcgaccgcgg agctccggac ggccgtcgag gcgctcctca
     1201 ccgccgagca gaaggcgaag ctggaagggt gcctgaaggc cccgcggccg aacgggcctg
     1261 ccggtgagga ggacgagggc gcggagcgct gagatccaag acaacgccag catcgacgcc
     1321 accgcgccct cggggcccac acgcccgggg gcgcgagggc gtgccgcgag ctgaaacgcg
     1381 gatgccgggg cctgggcgag agctcccgag atcggttccc tgaaggactg ggcaccgggc
     1441 tcgactcggt cggccgcgag ccccgcgcgc tcgccctgcg cgggcgcgac ccgcaacggg
     1501 cccgcctgcg cgggccgccg ggcgcgcgcg aactcctcgc ttcgcttcgt caaacagcgc
     1561 gcgcgcctga tcggcggcct gctcgtcgga cccgcgcggg tccccgcgcc tgctcggacg
     1621 ctcgctgcgc ggggcacacg gcctccctcg aagcgtcggg cgattgaacg ttccggaacg
     1681 gggcaggggc gccgaacggc tatccgtgcg cgacgcgccg cggccgtccc gcgggtgcgg
     1741 ctccgccggc ggcaggtgcc gcaggcttcg ccggcttccc ctcggtgtcc ccgtggctcg
     1801 ccgatgcggc gcgcttccgg aactcctcgg gggtgacccg agcgaagtgc cgcttcatcg
     1861 cgacctcggc cttcttcgga tcgagcattg gagttctctc ccgggagctg aaccagctcc
     1921 tcgcattcgt aggctcctag gaacctacgt tcctagccta gcacggtgag atcctcgatc
     1981 aacagcggat cgcggacagc gatctggacg tggctccggc tgtagagagc cgaagcgggg
     2041 aaaaggcgct cgccctccac gaacgctccc cggaggcagc ggacgttctg accgtcctcg
     2101 gcgaggacgc cgaccatgta gttcaggacc gcccggtcca gccggtgcgc gccgctcgtc
     2161 gcggtctgct tcggcaggac cttctcgcgt tcgacgaact gccggtgtga cgagctgagc
     2221 gtgggccacc actcgatgtc gaggaggtcg acacagtctt cgaggcggat cacggccctg
     2281 aggacggcgg cgttcgatct gccgtggtgc tcttccgccc attcgcgggc gcgaaggggc
     2341 gagtcctgga agaagtaaat gccgtccccg agccagtccc actcgttcgt gctgtacctc
     2401 agcccgttcg cgaggatcga ctcgaggcgg ccgacgtgcg tgccgtggta gcccgtcgcc
     2461 aggatgtgcc tcgcaggagc catctcggtg cgggcgccta cgccttcggc gggatcgtca
     2521 cgaggacctc ctcgccgagc atgtcgatga gcttcacggc gatcgtggcc tcgggacgag
     2581 gcggaggaag ctcgtaccgg ccttccacga ggtccgactt cttctcgggc atgtcggcca
     2641 ggaccacgtt gaagaccgct ccgtcgtagt cggtgtcgat caggatgcag tcgacgaccc
     2701 agcgccaatc ctcgatctcg ggtcggacga cgccggcctg gcgttcgagc cgctcgatga
     2761 tcgttgggga gacgaagtcg cggacctcga cgacgaggtg cttcttcgtc cgcgagacct
     2821 tcacgcgcgc cacggccggt tcgtgcttca ggagctttcc gtagcgctcg tcggtccgaa
     2881 gctcgatcac ctcgatccgg ttcacggcgc cgctcccctt ccggagccgg ttccagtcgt
     2941 cgacccaggc gcgtgcgccc acctcggcgc cgaggcagac caccttcacg gcgcggtcct
     3001 cctcgggccg cgtccccagc tcgcggcgga cttcctcgag gtcgagcggg ctgagggggt
     3061 gctgaaacgg gatgatccgg acgagggctt tcccgagcgt gccgtcgaag aagccgtccg
     3121 tccggacgcg ctccacgccc agcgcctcgc acgcgatttc gacggcctcg ttgtgctgga
     3181 tctgaaggtc gtagtcgttc acccgccagg tcgtgaaggc ggcctgcgcg gggcgagggc
     3241 catccggctc gagcgtcggc agatcggcct gcgccgcctc cacggtctgc tcctggatga
     3301 tcgactggac tctcttcgcc gtcgtctgga tcgcgccctt gttgatgtcg cacccgatcc
     3361 agcgccggcc cagcttctgc gcgacggccg cggtcgtgcc cgagccgatg aagcagtcga
     3421 ggacgaggcc gccggggttg gaccacacgg acagaagggc ctcaagtagg gcctccggct
     3481 tctgagtggg gtactccttc ttctgcttga cgtcggcggg gggaatcaga ggaatcggcc
     3541 agacgttgtc gagcgttcgg gagtcgctct cctggtagac gaggtctccg gattcgtcct
     3601 tcagattcac gagtcggcca tcgaccttaa cgcgtcgaag ctgccgaatg gccttgtcgc
     3661 gcggaacatc gacatcgtgg aagggtggcg tgcccttgga gaagatgagg atcgagttcg
     3721 tcgctcgcgg caggacgccc ttccgatcgt catgcatctt gttgtagaag taccagatca
     3781 cctcattctg gaacgcaccc tcgccgaatg tctcgtccag taggcatcgg aggtagtgcg
     3841 ccttccgcca gtcacaatgg agggcgaggg ccgaccgctc cgccatcagc tccttcagca
     3901 ggagtagccg ctcgtacatg aactggaggt agttgtcgtt cgcccagatg tccgtgtact
     3961 ggatctgctc gccgagggag taggtctcgc cagagatctt cgcggtgccc ttggggccgc
     4021 gaagggcgac gcggcggacg tagtcggctc cggagtcgaa gggagggtcg atgtagacga
     4081 ggtcgacctt gccgcggaag ccgttcgcga ggaggtgacc gaggacttcc ttgttgtctc
     4141 cgtggaagag gagtccgccc ttcgggtagc ccgagggcca gcccttccag ccgtctgcat
     4201 ccttggtgag cgagccggcc gggtcgaagg tctcgacgtg ctgggcgggg agggccggaa
     4261 cgtgagagag gggcttcttc cccacccacg agagcatcgg ccgccctttg gcggcggaga
     4321 tggccacggg ggccttcgcg gattcggtca cggcggttcc ttccgaagcg gggcgagcgt
     4381 ccggcgcgtc tggtcgacgg tgaccgtgtc gcccggggtg aagaggatct cgtagcggag
     4441 gcggtcgggg tcgagctgct cccagcggcg gatggcgagc gcctttcggc cggattcgcc
     4501 gtcggtgggg tgggcgcgat cgaactcctt cttgatctcc acgatcaccg cccggcccga
     4561 gccggccggg gcgccacgag cgggcttttt acggacgacg aagtccggcg tatagcggcg
     4621 ccacttcccc ttctcgtctt tgtactcgac gaagaagtcc gttctcgaag ggtcagtgag
     4681 ccctcccgtg aagtagacgt cctcgatctc gcccgggtcg agacgaagct cctcgagtac
     4741 gagctcgagg aagctcttct cggggttcga gtcgaaggcg taggggtcgt agtggaagcc
     4801 gaagtcgccc gggttgcggt cttccagcgt ctcgcgggcg aggaggaggc gctcgcggtc
     4861 tttcgggtag acgatctcgg ccgtgtagac ctcggccccg tccggcccga tctccttctc
     4921 gaagccggag ggcttgacca gcgcgagggc gacgtcgacg gcctcctcct tcacctcgtg
     4981 ctggccgagc tgtttctcga tctgtgcgct gaggtggggg atatcggcgt tcgggaccgg
     5041 cgcttccggg tagagacgcc ggacctcgtc gagcgcctcc cacaagtcga ggccgtggag
     5101 agccgcgagt cggacggcga cggtcatggc cgtagaagca tcgatctcgc tgtcgatctg
     5161 taccgcgtcg ccgacttggc ggaggacgcc ggccgaggcg acctgttcgg agatcgtgaa
     5221 ggtctttatc tcgagcccga catctccata ggccgccgga cgctcgagcc gaagcggtgt
     5281 gcgcgccttc gcgaccggaa cgacggtccg gagggtccgg cggacgcaga taggcgggat
     5341 atccaccttc cggagcacga tgcgggcgcg gccccgctcg acgcccgcgt ggtcgaggtc
     5401 ccggatcgat tcgccgtagg tctcctggag ctgtcggtcg aggatcgaga agttgtccgt
     5461 cgagaggtag acccgcgcct tgcgcgcgtt ccccgggatc tggcggaggc accgggtagc
     5521 ggcctgaagg acgaagttgt tcgaggactt gagctttcgg gcgagggcgc acgcgaagag
     5581 gctcgggcag ttccagcctt cggtcccctt gttgacgagg aggatgaccc ggtggggggc
     5641 cgccgggtcg ttgaggcgat tgaacgcctc gacgtccgcc tcgctcgtga gcctcgagtc
     5701 ggaggtgttc gtgaggcaga gcgccgggct gaggccgagg cgggcgaggg tctcgtcgat
     5761 gatgggtctg agctcgacga cgtcgtccgt ctgcgggaag tagatcgcga gctttgccgg
     5821 ggcgccgttc gggagacgga cttcgccgta gtcccgaaag aagtcctcga cgacgtgccg
     5881 caggtacggg ccgggctcgc cgtcgaagtc gtaggcctgg atgctcccgg cgaggtcttt
     5941 gaggatgccg tcgcggatgc cctgcgacag gccgtaccag gtgacgacgt cgcggagagg
     6001 ctggcgctgg aagtacgggg tcccggtcgt gttgacgacg cagaggacgt tcgtgttcgc
     6061 cgcgaggtag tcgacggtct tccggacctt cttgagatcg gcgtcgagcg actgaccgta
     6121 ggtgtggtgc gcctcgtcgg agaagacggc gaggtgggga aggctggcaa tcgcctggag
     6181 ccgcagattc gccacctcgc tcctcgcttc gtcctcgcgc ccctcgccga acagaggacc
     6241 gatgtcgccg cgccggacgt cctccttccg gatgcggatc ttctcggtgt tcgtcacgac
     6301 cacgttgaag agggagcccg gtgtgacggg gatccccttc tctccgtccc gggtgaacgt
     6361 cagcttcacc gaggcggcga aacgcttgtg gagccggggc gggaggatgc gctcgtaagg
     6421 aacctcgaga agctcccgga gcgactcgag gatcgtcttg ccgggagcga agacgagtgc
     6481 gttctggacg aaggggccgt cggggtgctc cagagccatg gcgaactcgg tcgcgacgat
     6541 ggcgccgatg agcatggtct tgccggcccc catcgcgagg gccaggatgt agctcgggta
     6601 gtcgagccgg atcgactcgc gcagcgcctc gagccggaag ctacggacga agtcgtcgtc
     6661 gtcgcgaatc cgcgagagaa cgccgttcgc gccctcgtcg agggcgatcg cctggatgcg
     6721 ggagtcggtg aggccgagcg ccgggaggag ctcggagttc cgggggaaga gcttccagta
     6781 aaggtcgagg atgtgcggtg tcccctcgac gagccggagg taccagtagg tctcgagggc
     6841 gaggagctgg ggcgggcgca ggaaccggag ccgcccggtc tcgggctcgc gtgcaaactc
     6901 gaggatctcc gcgatggccg aaaagggatc gcaggaatgg ccaccctgtc tccaggcgtc
     6961 cactttgcga gaaagcgcct cgtgaagaac ggccgcaggc atcgtcggag gagtttgcca
     7021 ccagacgagg ggcgggacgg cgggaacgcc cgaccggcgc tcccaccccg ccgccggcgg
     7081 gcgagagcaa cgagagctgc cctgaagcgc gcgtctcgtc gaagcgtcta cccggccggc
     7141 tccggcggag gcgcgaggac gacccggtcc cggccctgcc gcttggcctc gtagagggcg
     7201 gcgtcggccc gggcgacgag cgcctccgtc gtctcccccc gggcgtgctc ggcgacgccg
     7261 gcgctcgacg tcacgcgctc ggggagccct t
//
LOCUS       DAOONL010000151         7195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12326_length_7195_cov_16.123529, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000151 DAOONL010000000
VERSION     DAOONL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12326_length_7195_cov_16.123529"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLP50_15130"
     CDS             <1..948
                     /locus_tag="PLP50_15130"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPA52926.1"
                     /translation="PIAQLTKPYDEYRSKSLLDVPTSEIERVTVLRGPNRLVAVRDGA
                     GWRLEQPVADLASGSFLDQLLADLAGVRATEFPAVAAADMPRIGLSPPAAEVLLAKGK
                     GVLARLAFGAAKADAAGKLYASRDGVVMVVDDRAQETLGKELSAFREGRLLPLDTWLV
                     TRVVFESESLRAGAEKLDGAWRSAGRDVEGRIAEDLLERLGRAEVRRFVAAKDLGSIG
                     LAPVRRRAPAPAGSVEVLLEKAKAPVRVVFFATPPLAAEKLVAARVDGRGDTLLVDAS
                     VWDDVRSFAARLKAAGAAGDAPATQPGSSPTATPAGPRP"
     gene            complement(1003..2475)
                     /locus_tag="PLP50_15135"
     CDS             complement(1003..2475)
                     /locus_tag="PLP50_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPA52927.1"
                     /translation="MASSEHLEALKSPDWNQWRSEHAGITPDLTDIGLLGLDLSGKNL
                     AGADLSRSKLDGANLDGASLAGAHLQGTSLRGTSLQRADLSNTDLEYQTLDKVALVGA
                     SLRGVNLKNASVREANLTACDLSTAKLEGTTFSGSVFSFSRLVAANFRGVVIDGCVFE
                     ECDLSQAVFDGITYQRGKFDKSTLTLAFFRDAKLAGTSFRGVKGQRITFRGANLTGAD
                     FGGADLTSSDFERVIAQAVDFSEATLRKACFKDADLRRSILLGALLEETDLTQANLEK
                     LDLRTPKLKFPNFTGAKLAEANGVGANLRGGNYTEADLSRADLTEANLEGGIFQRAHL
                     EGATLRRANLKRARMAGVLAPGADFSGTNLLGAEAQDADFSGTDFRFSTLVGAVLEGA
                     LFKGANVAHADFSEAKLGGSSFVGAKGEGAKFTGQLHLKLVLAAGAFLGSSKKKVEKV
                     EDRKEQERKARVARLEREAAERDRSVATRPARGDADPPEK"
     gene            2507..4009
                     /locus_tag="PLP50_15140"
     CDS             2507..4009
                     /locus_tag="PLP50_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA52928.1"
                     /translation="MSAVIPVRGAERIRRALLGSYPLVYVQSWEEGRVERAVATLAQK
                     FYERPVPFAVWTCVEGWSGLPDAPDGTRDPVAALEAVLRANGPGFYLMKDLPALFDGR
                     ADVVRKLRDVYRQAKGKGKFVILVSPRLVLPEDLKKEVFLVDYDLPDEAEVRNAVTVL
                     GRRYLGEGGLSAADADRVVTVLRGLTIDELEHVFSKVFARTPRFDEGTFDELLAEKEQ
                     SSRKEGVLEYVPPRFTLDDVGGLENLKEWLRKRQHLFTREAVEAGLPVPKGLLMMGMS
                     GCGKSLSVKTIPALWNLPLFRLDMNLVFGTANPEATFHRALKTVEGLAPSILWIDEIE
                     MAITGGREAGGGDPALGRIFSTFLTWMQEKEALVFVAATANRIHLLPAEFIRKGRFDQ
                     VFFLDLPNETERKAIFSVHLKKAKADVSKFDVVFLAKATKGFNGAEIESVVQASAIDA
                     FNEKRPLDEDAVSRVITNTVPLSRTMDEQIKAIKSWAHNRALSASRESAT"
     gene            complement(4040..4699)
                     /locus_tag="PLP50_15145"
     CDS             complement(4040..4699)
                     /locus_tag="PLP50_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPA52929.1"
                     /translation="MSRLLPLFPLDAVLLPQTRLPFHVFEPRYREMLADALDGERLLG
                     LHTLAPDAPPLPDGRPALLPYGCAGEIVEHEPLPDGRSNIVLLGTFRYRIASEREGRP
                     YRRADVVEAPVAPLARGRAGGPGRRELRRLLARDVERLADSVGRSEARHLPPSLSDEG
                     LVNEAASRLGLGSEERYALLAMETLEERYGWVLDRVRGLQSRIDLLAPYRREGDDPRW
                     N"
     gene            complement(4696..5055)
                     /locus_tag="PLP50_15150"
     CDS             complement(4696..5055)
                     /locus_tag="PLP50_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPA52930.1"
                     /translation="MRAPLRIMIAVALITVGASSGCTTDPDPAARVWKRRCAACHGPD
                     GAGRTRFAEGRPYADLTDGRWKHGPDRAALRRLVAQGDPASTMPPFAGTLTPEEIDAI
                     VDWTLSLPDRAGRGSAR"
     gene            5072..5305
                     /locus_tag="PLP50_15155"
     CDS             5072..5305
                     /locus_tag="PLP50_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPA52931.1"
                     /translation="MANKDDRYSDNAAGKFYVDTQCIDCDVCRVTAPNNFARQEEKGY
                     SFVTKQPDTPEEEAQCQEAMDSCPVEAIGNDGE"
     gene            complement(5316..6266)
                     /gene="lipA"
                     /locus_tag="PLP50_15160"
     CDS             complement(5316..6266)
                     /gene="lipA"
                     /locus_tag="PLP50_15160"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPA52932.1"
                     /translation="MSGPPLPRFVPNPDRPVYGETPRPEWLKVRLVDNEDTRGILDLV
                     RRKHLNTVCQEAQCPNIFECWGRERTATFMLMGDVCTRRCGFCAVTQGIGRPLDPEEP
                     RHVAEAVKELGVRHAVVTSVNRDELPDGGAAHFAATIGAIRAANPGVTVEVLIPDFLG
                     SEAALRTVLEAAPEVLNHNTETVARLYRRVRPDAIYARSLELLARAAAWRDGSAPAMR
                     VKSGLMVGLGEELDEVLATMRDLRAAGTDTLTVGQYLQPHARRLPVERWWTPEEFARL
                     RDEGRAMGFAVVEAGPLVRSSYHARAALGAAGGGDAAAPR"
     gene            6348..>7195
                     /locus_tag="PLP50_15165"
     CDS             6348..>7195
                     /locus_tag="PLP50_15165"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPA52933.1"
                     /translation="MSGAEAGRGNAERDVVVRSDEVRAALAEGRGVVALESTILCHGL
                     PFPKSLETHRAAEAAVRREGAVPALVAVVDGVPRAGLDDAAAEALARAGSRVRKATTR
                     DLGLLAARGESGATTVAATMAIAAASGIRVFATGGIGGVHRGAGETFDVSADLLELSR
                     TRVVVVAAGAKAVLDLPKTLEALETLGVPVIGFGTAELPAFWMRSSGLQLEVRCDDAD
                     EVAGLVAAHWAWPASGGLLVANPVPAEAALPREVVEGAIERSLSAAREQGVAGKAVTP
                     FLLARLA"
BASE COUNT          960 a         2522 c         2669 g         1044 t
ORIGIN      
        1 ccgatcgcgc agctgacgaa gccgtacgac gagtaccgct cgaagagcct cctcgacgtt
       61 ccgacctccg agatcgagag ggtcacggtc ctccggggac cgaaccgcct cgtcgccgtt
      121 cgcgacggcg ccgggtggcg tctcgagcag ccggtcgcgg acctcgcgtc gggctcgttt
      181 ctcgaccagc tcctcgccga cctcgccggc gtccgggcga ccgagttccc ggccgtcgcc
      241 gcggctgaca tgccgcggat cggcctctcg cccccggccg ccgaggtcct cctcgcgaag
      301 gggaaggggg tcctcgcccg gctcgccttc ggcgccgcga aggccgatgc cgccgggaag
      361 ctctacgcct cacgcgacgg tgtcgtgatg gtcgtcgacg accgggcgca ggagacgctc
      421 gggaaggagc tctccgcgtt ccgcgaggga cgcctcctcc cgctcgacac gtggctcgtc
      481 acgcgcgtcg tcttcgagag cgaatcgctc cgggccggtg ccgagaagct ggacggggcg
      541 tggcgttcgg cgggccggga cgtcgagggc cggatcgccg aggacctcct cgagcgtctg
      601 ggccgggccg aggtgcggcg cttcgtggcc gccaaggacc tcggttcgat cggcctcgct
      661 cccgtgcgcc ggcgggcccc cgccccggct ggctccgtcg aggtgctcct cgagaaggcg
      721 aaggcgcccg ttcgcgtcgt cttcttcgcg acgccgccgc tcgcggcgga gaagctggtg
      781 gccgctcgcg tcgacgggcg gggcgacacg ctcctcgtcg acgcgtccgt ctgggacgac
      841 gtccgctcct tcgcggcccg gctgaaggcg gcgggtgctg ccggagatgc ccccgcgacg
      901 cagccgggat cgtctccgac cgcgacgccg gccggtcctc gtccctagcg gaccggcagc
      961 cgcgtcgtgg ccccggggcg cgctcccggc ggcgcgcccg ggctacttct cgggggggtc
     1021 ggcgtcgccg cgggccgggc gggtcgccac ggagcggtcc ctctccgcgg cctcgcgctc
     1081 gaggcgggcg acccgcgcct ttcgctcctg ctccttgcgg tcctcgacct tctcgacctt
     1141 cttcttcgag gagccgagga acgccccggc ggccaggacg agcttcaggt gaagctgccc
     1201 ggtgaacttc gccccctcgc ccttcgctcc gacgaagctg gatcctccga gcttggcctc
     1261 ggagaagtcg gcgtgcgcga cgttcgcccc cttgaagagc gccccctcga ggaccgcgcc
     1321 gacgagagtg gagaagcgga agtcggtgcc ggagaagtcg gcgtcctgcg cctccgcccc
     1381 gagcaggttc gttcccgaga agtcggcccc cggcgcgagg acgcccgcca tccgcgcccg
     1441 cttcaggttc gctcgccgga gcgtcgcccc ttcgaggtgc gccctctgga agatgccccc
     1501 ttcgaggttc gcctcggtca ggtcggcccg ggagaggtcc gcctcggtgt agttgccgcc
     1561 gcgcaggttc gcgccgacgc cgttcgcctc ggcgagcttc gccccggtga agttcgggaa
     1621 cttcagcttc ggcgtccgga ggtcgagctt ctcgaggttg gcctgggtca ggtcggtctc
     1681 ctcgaggagc gccccgagga ggatcgaccg gcgcaggtcg gcgtccttga agcaggcctt
     1741 ccggagggtc gcttcggaga agtcgaccgc ctgcgcgatc acgcgctcga agtcggaaga
     1801 ggtcaggtcg gccccgccga agtcggcgcc ggtcaggttc gccccgcgga acgtgatccg
     1861 ctgccccttc acgccccgga agctcgtccc cgccagcttc gcgtcgcgga agaaggcgag
     1921 cgtgagcgtc gacttgtcga acttcccgcg ctggtaggtg atgccgtcga agaccgcctg
     1981 ggagaggtcg cactcctcga agacgcagcc gtcgatcacg actccgcgga agttggccgc
     2041 cacgaggcgg ctgaacgaga agacgctccc cgagaatgtc gtcccctcca gcttcgccgt
     2101 cgagaggtcg caggccgtca ggttcgcctc gcggaccgag gcgttcttga ggttgacgcc
     2161 tcggagcgag gcgccgacga gggcgacctt gtcgagcgtc tggtactcga ggtcggtgtt
     2221 cgagaggtcg gcgcgctgaa ggctcgtccc gcgcagggag gtcccctgca ggtgcgctcc
     2281 cgcgaggctc gcgccgtcga ggttcgcgcc gtcgagcttc gatcgcgaga ggtcggcgcc
     2341 ggcgaggttc ttcccggaga ggtcgaggcc gaggagcccg atgtcggtga ggtcgggggt
     2401 gatcccggcg tgctcggacc tccactggtt ccagtcggga ctcttcagtg cttccagatg
     2461 ctctgagctg gccatggcgt ggaagtctac cgtctagaat cgcgccatga gcgccgtcat
     2521 tcccgtccgt ggtgccgaga ggatccgccg cgccctcctc ggctcctatc ccctcgtcta
     2581 cgtgcagtcc tgggaggagg ggagggtcga gcgagcggtc gcgacgctcg cgcagaagtt
     2641 ctacgaacgg cccgttccgt tcgccgtctg gacgtgcgtg gaaggatggt cgggtctccc
     2701 ggacgctccc gacgggaccc gtgaccccgt cgccgccctc gaggcggtcc tccgcgcgaa
     2761 cggaccgggc ttctacctga tgaaggacct tcccgcgctg ttcgacgggc gggccgacgt
     2821 cgttcgcaag ctgcgcgacg tctaccggca ggcgaagggg aaggggaagt tcgtgatcct
     2881 cgtctcgccg cggctcgtcc ttcccgagga cctcaagaag gaggtcttcc tcgtcgacta
     2941 cgacctcccc gacgaggcgg aggtgcgaaa cgccgtcacg gtcctcggca gacggtatct
     3001 cggagaaggg gggctctcgg ccgcggacgc ggaccgggtc gtcaccgtcc tgcgcggcct
     3061 gacgatcgac gagctcgagc acgtcttctc gaaggtcttc gcccggacgc cgcgcttcga
     3121 cgaggggacg ttcgacgagc tcctcgccga gaaggagcag tcctcgagga aggagggcgt
     3181 cctcgagtac gtccctccgc gcttcacgct cgacgacgtc ggcggcctcg agaatctgaa
     3241 ggagtggctg cgaaagcggc agcacctctt cacccgcgag gcggtcgagg cgggtctccc
     3301 ggtcccgaag gggctcctga tgatggggat gtcgggctgc gggaagtcgc tctcggtcaa
     3361 gacgattccg gcgctgtgga acctcccgct cttccgcctc gacatgaacc tcgtcttcgg
     3421 cacggcgaac ccggaggcga cgtttcaccg ggccctgaag accgtggaag gactcgcgcc
     3481 gtccatcctc tggatcgacg agatcgagat ggcgatcacc ggcgggcgcg aagccggcgg
     3541 aggcgacccg gcgctcgggc ggatcttctc gaccttcctc acctggatgc aggagaagga
     3601 ggccctcgtc ttcgtcgcgg cgaccgcgaa ccggatccac ctcctgccgg ccgagttcat
     3661 ccggaagggg cgcttcgacc aggtcttctt cctcgacctc ccgaacgaga cggaacggaa
     3721 ggcgatcttc tcggtccacc tgaagaaggc gaaggccgac gtctcgaagt tcgacgtcgt
     3781 cttcctcgcg aaggcgacga agggcttcaa cggcgcggag atcgagtcgg tcgtccaggc
     3841 ctcggcgatc gacgcgttca acgagaagcg gccgctcgac gaggacgccg tctcgcgcgt
     3901 catcacgaac accgtcccgc tctcgcggac gatggacgag cagatcaagg cgatcaagag
     3961 ctgggcgcac aaccgggccc tctcggcttc ccgcgagagc gccacctgac gggtcgcccc
     4021 ggaggggggg cggtcgcctt cagttccagc gcgggtcgtc gccctcgcgc cggtagggag
     4081 cgaggaggtc gatccgcgac tggaggcctc ggacgcggtc gagcacccat ccgtaccgct
     4141 cttcgagggt ctccatcgcg agcagggcgt accgctcctc cgacccgagg ccgaggcggc
     4201 tcgccgcctc gttgacgagc ccctcgtcgg agagcgacgg agggaggtgc cgcgcctcgc
     4261 tccggccgac ggagtcggcg agacgctcga cgtcgcgcgc gaggaggcgc cggagctcgc
     4321 gccggccggg gccgccggcg cgtccgcgcg cgagcggcgc cacgggcgcc tctacgacgt
     4381 cggcccgccg gtacgggcgg ccctccctct cggacgcgat ccggtaccgg aacgtcccga
     4441 ggaggacgat gttcgaccgc ccgtcgggaa gcggctcgtg ctccacgatc tccccggcgc
     4501 agccgtaggg gaggagggcg ggacgtccgt cggggagggg cggagcgtcc ggcgcgagcg
     4561 tgtggagtcc gaggaggcgc tccccgtcga gagcgtcggc gagcatctcg cggtatctcg
     4621 gctcgaagac gtggaacgga agccgggtct gcgggaggag cacggcgtcg agcgggaaga
     4681 gggggaggag ccggctcatc gcgcgcttcc tcgccccgcc cggtcgggaa gcgacagggt
     4741 ccagtcgacg atcgcgtcga tctcctccgg cgtgagggtc ccggcgaacg ggggcatcgt
     4801 gctcgccggg tctccctggg cgacgagccg gcggagggcc gcccggtcgg gaccgtgctt
     4861 ccaccgcccg tcggtcaggt cggcgtacgg gcgcccctcc gcgaagcggg tccggcccgc
     4921 cccgtccggc ccgtggcagg cggcgcagcg cctcttccag acgcgggccg cggggtcggg
     4981 gtcggtcgtg cagccggagg acgctccgac ggtgatcagg gcgacggcga tcatgatccg
     5041 aagcggagct ctcatgctat cttccgcggc catggcgaac aaggacgacc gctactccga
     5101 caacgctgcc ggcaagttct acgtcgacac ccagtgcatc gactgcgacg tctgccgggt
     5161 gacggccccc aacaacttcg cgcgtcagga agagaagggc tattccttcg tgacgaagca
     5221 gcccgacacc cccgaggaag aggcccagtg ccaggaggcg atggactcct gcccggtcga
     5281 ggcgatcggg aacgacgggg agtagcaccg gccggtcagc gcggggcggc ggcatcgccg
     5341 cccccggccg cgccgagcgc cgcccgggcg tgatagctcg agcggacgag cggtcccgcc
     5401 tccaccacgg cgaatcccat cgcccttccc tcgtcgcgca gccgcgcgaa ctcctccggc
     5461 gtccaccatc tctccaccgg tagcctccgc gcgtgtggct ggaggtactg gccgacggtg
     5521 agcgtgtcgg tcccggcggc gcgcaggtcg cgcatcgtgg cgagcacctc gtcgagctcc
     5581 tccccgaggc cgaccatcag cccgctcttc acgcgcatcg ccggagccga tccgtcgcgc
     5641 caggcggcgg cgcgtgcgag gagctcgagc gaccgggcat agatcgcatc ggggcggacg
     5701 cgccggtaga ggcgcgcgac cgtctcggtg ttgtggttca ggacctccgg cgccgcctcg
     5761 aggaccgtcc ggagcgccgc ctcgctcccg aggaagtcgg ggatcagcac ctcgaccgtg
     5821 acgccggggt tcgccgctcg aatcgctccg atcgtcgcgg cgaagtgggc cgcgccgccg
     5881 tcggggagct cgtctcggtt gacggacgtg acgaccgcgt gccggacgcc cagctccttc
     5941 accgcctcgg cgacgtggcg cggctcctcc gggtcgagcg gccggccgat cccctgggtc
     6001 acggcgcaga agccgcagcg gcgcgtgcag acgtcgccca tgagcatgaa cgtcgccgtc
     6061 cgctcgcggc cccagcactc gaagatgttc gggcactgcg cctcctggca gacggtgttc
     6121 aggtgcttcc ggcggacgag gtcgaggatg ccccgcgtgt cctcgttgtc gacgagccgg
     6181 actttcagcc actcggggcg cggggtctcg ccgtagaccg gccggtcggg gttcgggacg
     6241 aatcggggaa ggggcggacc gctcacgggg gcggagtgta gcctccggag gctgcggggc
     6301 cggaggcgcg gggagctcgg cgggccgggc ccgggaagga gagcgtggtg agcggagcgg
     6361 aggcgggaag agggaacgcg gagcgcgacg tcgtcgtccg ctcggacgag gtgcgcgcgg
     6421 ccctcgcgga ggggcgcggc gtcgtcgcgc tcgagtcgac gatcctctgc cacggcctgc
     6481 cgtttccgaa gagcctcgag acgcaccgcg ccgccgaggc ggcggtgcgg cgggaggggg
     6541 ccgtgccggc gctcgtcgcc gtcgtggacg gcgtgccacg ggccgggctc gacgacgcgg
     6601 cggcggaggc gctcgcgcgc gccggctcgc gcgtgaggaa ggcgacgacg cgcgacctcg
     6661 gcctcctcgc cgcgcgtggg gagagcgggg cgacgacggt cgcggcgacg atggcgatcg
     6721 ccgcggccag cggcatccgc gtcttcgcga cgggcgggat cggcggcgtc caccgaggtg
     6781 ccggggagac gttcgacgtc tcggccgacc tcctcgagct ctcgcgcacg cgtgtcgtcg
     6841 tcgtcgccgc cggggcgaag gcggtcctcg acctgccgaa gacgctcgaa gcgctcgaga
     6901 cgctcggcgt cccggtcatc ggcttcggga cggcggagct cccggcgttc tggatgcgta
     6961 gctcgggcct gcagctcgag gtccgctgcg acgacgcgga cgaggtggcc ggcctcgtgg
     7021 ccgcgcactg ggcctggccg gcgagcggag gcctcctcgt cgccaatccc gtcccggccg
     7081 aggcggcgct ccctcgtgag gtcgtggagg gcgcgatcga gcggagcctc tcggcggcgc
     7141 gggagcaggg ggtcgccggc aaggccgtca cgccgttcct cctcgcgcgc ctcgc
//
LOCUS       DAOONL010000152         7178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12367_length_7178_cov_18.946231, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000152 DAOONL010000000
VERSION     DAOONL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12367_length_7178_cov_18.946231"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="clpX"
                     /locus_tag="PLP50_15170"
     CDS             <1..932
                     /gene="clpX"
                     /locus_tag="PLP50_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPA52934.1"
                     /translation="DVELQKSNILLIGPTGTGKTLLAQTLAKMLSVPFTIADATTLTE
                     AGYVGEDVENIILKLYQAAGQDKEKTERGIVYIDEIDKIARKGENPSITRDVSGEGVQ
                     QALLKILEGTVTNVPPQGGRKHPHQEFIQIDTTNILFICGGAFVGLEDLIARRLTEKQ
                     MGFGAKIESRKQKRSGATLEKVHPEDLIKFGMIPEFIGRVPVTATLHDLDKAALVSIL
                     KEPKNSLLKQYQTLLEFENVGLRFTDDAVEEVADEAMKRTLGARGLKVILEELMLDVM
                     YRVPSEEEIEECVITKDVVQRRAAPILSLRKVG"
     gene            1009..>1940
                     /locus_tag="PLP50_15175"
     CDS             1009..>1940
                     /locus_tag="PLP50_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPA52935.1"
                     /translation="MTNPATPSQRPATRTLPLVPLRDMVVFPRMMAPFVVGRASSIAA
                     LEAALLTTDKQIFLAAQRDPKIDEPAAGDIHDVGVVATVVQSLKLPNGHIKVMVEGVT
                     RGVIRAFATTGGHKEVEVAPAEARALPGGAAELGPYMQKVLGVFEQYAKLSHQLAYEG
                     LLASLTLDDPDVFADMLAGHLPASIPTPEKQALLETVNSLERLQKLQDLLEIEIEKIN
                     IDRRINNKVKKQMERAQKEYYLNEKIKAIHQELGRKDEKSDELEELRQKIDAAGMPKD
                     VKEKAVAELKRLEAMPNVSAEATVSRNYIDWLVNV"
     assembly_gap    1941..2040
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2041..3279
                     /locus_tag="PLP50_15180"
     CDS             <2041..3279
                     /locus_tag="PLP50_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPA52936.1"
                     /translation="EVRGHRRTYIGAFPGQIIQLMKKAGTVNPVFLLDEVDKMSMDFR
                     GDPSAALLEVLDPEQNHAFVDHYLDVEYDLSKVMFIATANVIHPIPPALKDRMEIISL
                     SGYTPNEKLAIARQFLVPKQVKENGLTPEQARFDDSGIYGLVEKYTREAGVRNLEREI
                     ASVCRKIARKVVADEAGAAKEGITVTAENLPDFLGKPKHRPAKKGEKAEVGLSTGLAW
                     TEAGGDVLHIETTLMRGKGNLVLTGQLGDVMQESARAALSYVRSRAEMYGADADFYEK
                     KDIHVHIPEGAIPKDGPSAGITMATSILSAVTNIACRKDLAMTGEITLRGKVLPVGGI
                     KEKLLAAYRAGITTIVLPRENEKDLDDLPEEIRNVLETHLVDDVDEVVRLALEGSPVA
                     TPPKPVTAETGTTPPATLAH"
     gene            3344..4051
                     /gene="yihA"
                     /locus_tag="PLP50_15185"
     CDS             3344..4051
                     /gene="yihA"
                     /locus_tag="PLP50_15185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPA52937.1"
                     /translation="MTRKENPVSRTPKAARAVSVRSPLGKSVRLELPAHSAGERPRGL
                     PIPAVAVLGRSNVGKSSLLNALLATKVARVSQTPGRTQALHWYRVDDAFHVVDCPGYG
                     WAKTARASRERFSGLIEELLTGERAPNLALLLVDGRLPPQASDVSMALFLRHAGVATV
                     VAVTKWDAVKPSQRVRHLRALAAELEDSERPLLPVSSETGENLPTLGRLLKDRLTGRT
                     REGAVPAAPQGPTQKES"
     gene            4162..5409
                     /gene="rho"
                     /locus_tag="PLP50_15190"
     CDS             4162..5409
                     /gene="rho"
                     /locus_tag="PLP50_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPA52938.1"
                     /translation="MLDIRTLHNTPMPQLLKIAQELEVEGAAALRKQELIFKVLQAQT
                     ERMGLIFSEGVLEVLPDGFGFLRAPEYNYLAGPDDIYVSPSQIRKFGLRTGDTISGQI
                     RPPKEGERYFALIKVEAVNFEHPDVAREKILFDNLTPLYPQERIRLETPNMSSRVLDL
                     IAPVGKGQRGLIVAPPRTGKTMLLQALANSITTNHPEVTLIVLLIDERPEEVTDMQRS
                     VRGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTTCPP
                     SGKVLSGGVDANALQKPKRFFGAARNVEEGGSLTIMATALIDTGSRMDDVIFEEFKGT
                     GNMEIHLDRRLVDRRVFPSIDINKSGTRKEELLIEKAELNRIWILRKVLTPLSTVEAM
                     ELLLERLEKSKTNLEFLSSMSAG"
     gene            5475..6284
                     /locus_tag="PLP50_15195"
     CDS             5475..6284
                     /locus_tag="PLP50_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPA52939.1"
                     /translation="MTPAIPVSVVDFVNAWPVTWGFLKGAVEGFLPINDVPSVCAERL
                     ARGEVQAGIIPAIEAARIPGVRIVRGVGIAAYDRVRSVLLVSRVPFEEVRSVALDTSS
                     RSSAAMARILLADLFGASPEYHRAVPDLPRMLSSADAALLIGDPALTADLGGHHVLDL
                     AEGWSRLTGLPLVTAVWAVRRDVSPEPFLWSRDYARHRMPEILDAAMARTGLDRRLLT
                     EYFEMNLHYDLDADDERGLAEFYRRAAVHGLIPSPDLPPFAGVPLVPRSFS"
     gene            6284..>7178
                     /locus_tag="PLP50_15200"
     CDS             6284..>7178
                     /locus_tag="PLP50_15200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52940.1"
                     /translation="MASAAPATFLPNPPSPGLGGSLRAAALLERAASGGRLTPSEAVL
                     LLEEAPLLELGAAAAAVRRRLHPGDVATYQVDRNINYTNVCVYRCAFCAFYRKAGDEE
                     AYVLPLDEIGAKVEETLALGGTGVLLQGGVHADLPFSFYEELLSYLRDRYPGIHRHAF
                     SAPEIWFLAKKEKLPVAEVLVRLREAGLMSVPGGGAEILEDGVRKRIVALAKAPTERW
                     AEVHREAHRLGMPTTATMMFGVGETYAERVAHLDVVRRTQDASLEAGKESFTAFIPWT
                     FQEENTELDGKVEVSTGHDYLR"
BASE COUNT         1190 a         2546 c         2372 g          970 t
ORIGIN      
        1 tggacgtcga gctccagaag tcgaacatcc tcctcatcgg gccgacgggc accgggaaga
       61 cgctcctcgc ccagacgctg gcgaagatgc tctcggtgcc gttcacgatc gccgacgcga
      121 cgacgctcac cgaggcgggg tacgtcgggg aggacgtcga gaacatcatc ctgaagctct
      181 accaggcggc gggtcaggac aaggagaaga ccgagcgcgg catcgtctac atcgacgaga
      241 tcgacaagat cgcccgcaag ggggagaacc cttcgatcac gcgtgacgtc tccggcgagg
      301 gggtccagca ggccctcctg aagatcctcg aggggacggt cacgaacgtc ccgccgcagg
      361 gcggtcgcaa gcacccgcac caggagttca tccagatcga cacgacgaac atcctcttca
      421 tctgcggggg ggcgttcgtc ggcctcgagg acctcatcgc gcgccggctg accgagaagc
      481 agatgggctt cggcgcgaag atcgagtcga ggaagcagaa gcgctcgggg gccacgctcg
      541 agaaggtcca cccggaggac ctgatcaagt tcgggatgat cccggagttc atcggccgcg
      601 tcccggtgac ggcgacgctc cacgacctcg acaaggcggc gctcgtctcc atcctgaagg
      661 agccgaagaa ctccctcctg aagcagtacc agacgctcct cgagttcgag aacgtcgggc
      721 tgaggttcac cgacgacgcc gtggaggagg tcgccgacga ggcgatgaag cgcacgctcg
      781 gcgcgcgcgg cctcaaggtg atcctcgagg agctcatgct cgacgtgatg taccgcgtcc
      841 cctccgagga ggagatcgag gagtgcgtca tcacgaagga cgtcgtccag cggcgcgccg
      901 cgccgatcct gtcgctccgg aaggtcggtt gacggccgtt cccgggcggg cgagcggccc
      961 cgcgccccgc gcgcgaggcc gtggccctga agccacgagg gtgaccccat gacgaatccc
     1021 gcgaccccgt cccaaaggcc cgcgacgcgg acgctgccgc tcgtgccgct ccgggacatg
     1081 gtcgtcttcc cccggatgat ggcccccttc gtcgtcggcc gcgcctcttc gatcgcggcg
     1141 ctcgaggcgg cgctcctgac gaccgacaag cagatcttcc tcgccgccca gcgcgacccg
     1201 aagatcgacg agcccgccgc gggcgacatc cacgacgtcg gcgtcgtggc gacggtcgtg
     1261 cagtcgctca agctcccgaa cggccacatc aaggtgatgg tcgagggggt gacgcgtgga
     1321 gtcatccgcg cgttcgcgac gacgggcggc cacaaggagg tcgaggtcgc gcccgcggaa
     1381 gcgcgggccc tgccgggcgg cgccgccgag ctcgggccat acatgcagaa ggtgctcggc
     1441 gtcttcgagc agtacgccaa gctctcgcac cagctcgcgt acgaggggct cctcgcctcc
     1501 ctcacgctcg acgaccccga cgtcttcgcc gacatgctcg cgggccacct cccggcgtcg
     1561 atcccgacgc ccgagaagca ggccctcctc gagacggtga actccctgga gaggctccag
     1621 aagctgcagg acctgctgga gatcgagatc gagaagatca acatcgatcg ccggatcaac
     1681 aacaaggtca agaagcagat ggagcgggcc cagaaggagt actacctcaa cgagaagatc
     1741 aaggcgatcc accaggagct gggccgcaag gacgagaagt ccgacgagct cgaggagctg
     1801 cgtcagaaga tcgacgccgc ggggatgccg aaggacgtca aggagaaggc cgtcgccgag
     1861 ctgaagcggc tcgaggcgat gccgaacgtc tcggccgagg cgacggtctc gcgcaactac
     1921 atcgactggc tcgtgaacgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 gaggtccgcg gccaccgccg gacctacatc ggcgccttcc ccggccagat catccagctg
     2101 atgaagaagg ccgggacggt gaatccggtc ttcctgctcg acgaggtcga caagatgtcg
     2161 atggacttcc gcggcgaccc gtccgcggcg ctcctcgagg tcctcgaccc cgagcagaac
     2221 cacgcgttcg tcgaccacta cctcgacgtg gagtacgacc tctccaaggt catgttcatc
     2281 gcgaccgcga acgtgatcca cccgatcccg cccgccctga aggaccggat ggagatcatc
     2341 agcctctcgg gctacacgcc gaacgagaag ctggcgatcg cccgccagtt cctcgtcccg
     2401 aagcaggtca aggagaacgg gctgacgccc gagcaggccc gcttcgacga ctcggggatc
     2461 tacggcctcg tcgagaagta cacccgcgag gcgggcgtgc ggaacctcga gcgcgagatc
     2521 gcgtccgtct gccggaagat cgcccgcaag gtcgtcgcgg acgaagccgg ggcggcgaag
     2581 gaggggatca ccgtcacggc ggagaacctc cccgacttcc tcggcaagcc gaagcaccgc
     2641 cccgcgaaga agggggagaa ggccgaggtc ggcctctcca ccgggctcgc ctggaccgag
     2701 gccggcggcg acgtcctcca catcgagacg accctgatgc gcggcaaggg gaacctcgtc
     2761 ctgacgggcc agctcggcga cgtgatgcag gaatcggccc gcgcggcgct ctcgtacgtg
     2821 aggagccggg ccgagatgta cggcgccgac gccgacttct acgagaagaa ggacatccac
     2881 gtccacatcc cggagggggc gatcccgaag gacggcccgt cggccgggat cacgatggcc
     2941 acgtcgatcc tctccgccgt cacgaacatc gcctgccgca aggacctcgc catgacgggc
     3001 gagatcacgc tccgggggaa ggtcctcccc gtgggcggca tcaaggagaa gctcctcgcc
     3061 gcctatcgcg cggggatcac gacgatcgtc ctcccccggg agaacgagaa ggacctcgac
     3121 gacctcccgg aggagatccg gaacgtcctc gaaacgcacc tcgtcgacga cgtcgacgag
     3181 gtcgtccgcc tcgcgctcga gggctccccg gtcgcaaccc cgccgaagcc ggtcacggcc
     3241 gagacgggga cgacgccccc cgccaccctg gctcactgat ccgccggccg gcccccgcgc
     3301 gggggccggc cggtgaggcg ccccttttcc ttcagcttcg aatatgaccc ggaaagagaa
     3361 ccccgtttcc cgcacgccca aggccgcgcg ggcggtctcc gtgaggagtc cgctcgggaa
     3421 gtcggtccgc ctcgagctcc cggcccacag cgccggcgag cggccgcgcg gcctgccgat
     3481 cccggccgtc gccgtcctcg gccgctcgaa cgtcgggaag tcgagcctcc tgaacgccct
     3541 cctcgccacg aaggtggccc gcgtctcgca gacccccggg cggacgcagg cgctccactg
     3601 gtaccgggtc gacgacgcct tccacgtcgt cgactgcccc ggctacggct gggcgaagac
     3661 ggcccgcgcc tcgcgggagc gcttctcggg gctcatcgag gagctcctca cgggcgagcg
     3721 ggcgcccaac ctcgctctcc tcctcgtcga cggccggctc cccccgcagg cgtcggacgt
     3781 ctcgatggct ctcttcctcc ggcacgccgg cgtcgcgacc gtcgtcgccg tgacgaagtg
     3841 ggacgccgtg aagccgtcgc agagggtgcg gcacctgcgg gctctcgccg ccgagctgga
     3901 agattccgaa cgccccctcc tgcccgtttc gtccgaaaca ggcgaaaatc tgcccaccct
     3961 cggccgtctt ctcaaggacc ggctcaccgg aaggacccgg gagggcgccg tgcccgcggc
     4021 gccgcagggt ccgacacaga aggagtcctg atgccccgcc gcaaggtccc cggaaccgac
     4081 tacgtcagcc cgacgatcga cccgaacgac ctcccgaacg acctcgagga gccggtctcg
     4141 accgacctcg agaacgcgcc gatgctcgac atccggacgc tgcacaacac cccgatgccg
     4201 cagctcctga agatcgcgca ggagctggag gtcgaggggg ccgcggcgct ccgaaagcag
     4261 gagctcatct tcaaggtcct gcaggcgcag acggagcgga tgggcctcat cttctcggaa
     4321 ggggtcctcg aggtgctgcc ggacggcttc gggttcctgc gcgcccccga gtacaactac
     4381 ctcgccgggc cggacgacat ctacgtctcg ccctcccaga tccggaagtt cggcctccgg
     4441 acgggcgaca cgatctcggg gcagatccgc ccgccgaagg aaggcgagcg ctacttcgcc
     4501 ctcatcaagg tcgaggcggt gaacttcgag caccccgacg tggcgcgcga gaagatcctc
     4561 ttcgacaacc tgacgccgct ctacccgcag gagcggatcc ggctcgagac gccgaacatg
     4621 tcctcgcgcg tcctcgacct gatcgccccg gtcggcaagg ggcagcgcgg cctcatcgtc
     4681 gcgccgccgc ggaccggcaa gacgatgctc ctgcaggccc tcgcgaactc gatcacgacg
     4741 aaccaccccg aggtgacgct catcgtcctc ctgatcgacg agcggcccga ggaggtcacc
     4801 gacatgcagc ggtcggtgcg gggcgaggtc atcagctcga cgttcgacga gccggcgacg
     4861 cgccacgtgc aggtggccga gatggtcatc gagaaggcga agcgcctcgt cgagcacaag
     4921 cgcgacgtcg tcatcctcct cgactcgatc acgcgcctcg cccgcgccta caacacgacc
     4981 tgcccgccgt ccgggaaggt cctctccggc ggcgtcgacg cgaacgcgct ccagaagccg
     5041 aagcgcttct tcggcgcggc gcgcaacgtc gaggagggcg ggtcgctcac gatcatggcg
     5101 acggccctca tcgacaccgg ctcgcggatg gacgacgtca tcttcgagga gttcaagggg
     5161 acggggaaca tggagatcca cctggatcgc cggctcgtcg accgccgcgt cttcccgtcg
     5221 atcgacatca acaagtcggg gacgcggaag gaggagctcc tcatcgagaa ggcggagctc
     5281 aaccggatct ggatcctccg caaggtcctc acgccgctct cgacggtcga ggcgatggag
     5341 ctgctcctcg agaggctcga gaagtcgaag acgaacctgg agttcctctc gtcgatgtcg
     5401 gcggggtaga ccgatcggcc ggcgtctccg tccacgtccg acccgcgggg cggcatcgta
     5461 agatcgacgt ggacatgacg cccgccatcc ccgtctccgt cgtcgacttc gtcaacgcct
     5521 ggccggtcac ctggggcttc ctgaaggggg cggtcgaggg tttcctcccg atcaacgacg
     5581 tcccttctgt ctgcgcggag cggctcgcgc gcggcgaggt gcaggcgggg atcatcccgg
     5641 cgatcgaggc cgcgcggatc cccggcgtgc ggatcgtccg cggcgtcggc atcgccgcgt
     5701 acgaccgcgt gcggagcgtc ctcctcgtct cgcgggtgcc gttcgaggag gtgcggagcg
     5761 tcgcgctcga cacgagctcg cgctcctcgg ccgcgatggc gaggatcctc ctcgccgacc
     5821 tcttcggcgc ctcgcccgag taccaccggg ccgtccccga cctgccgcga atgctctcga
     5881 gcgccgacgc cgcgctgctg atcggcgacc cggcgctcac ggccgacctc ggcggacacc
     5941 acgtcctcga cctggccgag gggtggagcc ggctgacggg gctcccgctc gtgacggcgg
     6001 tctgggccgt ccgccgggac gtctcccccg agccgttcct ctggtcacgc gactacgcgc
     6061 gccaccggat gcccgagatc ctcgacgccg cgatggcccg gacggggctc gaccggaggc
     6121 tcctgacgga gtatttcgag atgaacctcc actacgacct cgacgccgac gacgagcgcg
     6181 gcctcgcgga gttctaccgg cgcgccgccg tccacgggct gatcccgtcc cccgacctcc
     6241 cgccgttcgc cggcgtgccg ctcgtcccga ggtccttctc ctgatggcct ccgccgcgcc
     6301 ggccacgttc ctcccgaacc ctccgtcccc cggcctcgga ggctcgctgc gggccgccgc
     6361 gctgctcgag cgcgccgcgt ccggcgggcg gctcacgcct tcggaggcgg tcctcctcct
     6421 cgaggaggcg ccgctcctcg agctgggcgc cgcggccgcc gccgtgcggc gccgcctcca
     6481 cccgggcgac gtcgcgacgt accaggtcga ccgcaacatc aactacacga acgtctgcgt
     6541 ctaccgctgc gccttctgcg ccttctaccg gaaggccggg gacgaggagg cctacgtcct
     6601 cccgctcgac gagatcggcg cgaaggtgga ggagacgctc gcgctcggcg ggacgggagt
     6661 cctcctgcag ggcggcgtcc acgccgacct gccgttctcg ttctacgagg agctcctctc
     6721 gtacctgcgg gaccgctacc ccggcatcca ccgccacgcc ttctcggcgc cggagatctg
     6781 gttcctcgcc aagaaggaga agctccccgt cgccgaggtc ctcgtgaggc tgcgcgaggc
     6841 ggggctgatg tcggtgcccg ggggcggcgc cgagatcctc gaggacggcg tccggaagcg
     6901 gatcgtcgcg ctcgcgaagg cgccgacgga gaggtgggcc gaggtacacc gcgaggcgca
     6961 ccggctcggg atgccgacga cggcgacgat gatgttcggc gtcggcgaga cgtacgcgga
     7021 gcgggtcgcg cacctcgacg tcgtgcgccg cacccaggac gcctcgctcg aagccgggaa
     7081 ggagtcgttc acggcgttca tcccctggac gttccaggag gagaacaccg agctcgacgg
     7141 gaaggtcgag gtctcgaccg gccacgacta cctccgga
//
LOCUS       DAOONL010000153         7088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12555_length_7088_cov_15.149865, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000153 DAOONL010000000
VERSION     DAOONL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_7088_cov_15.149865"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="PLP50_15205"
     CDS             complement(<1..2848)
                     /locus_tag="PLP50_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA52941.1"
                     /translation="MTRARAALPGAVLLASAALFASPGLAQPGPSAPLAAVHHELDVS
                     FVPATGFLRVAAEVSLPPGAKPELLLNGALKVTRSSPALKEVPLGDVAAFFGNNAAAT
                     EPSRRVTLKRYRASGAPATLTLTYEGIVRNELTAPKEEYQRGFRETPGTIGPEGIYLA
                     GSTFWVPVFGEALASFRMTAKVPDGWQLVSQGDGTSRGADGLARWDSKGPVDEAYLVG
                     GPLVVYRDRAGDVETLAFLRTKDDALAAKYLAATAQYLEMYRGLIGPYPYGKFALVEN
                     FWETGYGMASFTLLGPQVIRFPFILASSYPHEILHNWWGNSVFVDYAKGNWCEGLTAY
                     LADHLIQEQRGKGEEYRRATLQKYRSYVRDGRDFPLTAFRGRESAATEAVGYGKALMG
                     FHMVRRHLGDAAFRKVLARFYADFRGRRATFDDFRRTAEAVSGRPLSALFDPWVTRPG
                     AATLALGAATVAKTGSGWTVGGSLRQTQEDDPFPLEVPVAVVTEKGTIRKVVPLDGRE
                     ARFEVATDAEPLALAADPQFDLFRRLDPREIPPSIGQLFGEPKVLAILPAGLSAEEDA
                     AWRALLSGWTTPTHDVEVKAESEVSSLPGDRAVWVVGKANRFASLFAKQEGLAVGATE
                     LVALGEKAALAGHTFAFVARHPGDPGKAAGWLFGEPLAALPGLGRKLPHYGKYSFVGF
                     EGTEPANVLKGQWPESDSPLRADLRPAERRAAPLPALAPESRKALAELPPAFSQARLA
                     AHVAALAAPALEGRGLGTAGERSAAEYVAAGMKAAGLSPAGDEGTFFQRFRVEKTASG
                     APADVANVVGLLPGSDPAWKEESVVVVAHYDHLGRGWPDARSEFAGQVHPGADDNASG
                     VAMLLELARAMKDEKPRRSVVFLAVTGEEAGRLGSMRWVAGPSSRDAKKTLAAVNLDT
                     VGRLGSGKLQVLGTGTAGEWIHAVRGASWVTGVET"
     gene            complement(2845..4095)
                     /locus_tag="PLP50_15210"
     CDS             complement(2845..4095)
                     /locus_tag="PLP50_15210"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPA52942.1"
                     /translation="MRRALCTALLLAFAATSLYADEAALDLPLGDPARREKTLTPSLD
                     VVLDARTGAAISPAEVATRLAKSRLVFLGESHTNAEFHRVQLQVIRELVRAGRKVLVG
                     LEMYPATEQAALDLWNAGSLSEAEFVERSDWYRSWGYHWDYYRDVFLFCREAKVTLHA
                     VNVPRELVSDVRKKGFDGLTVEQKSLLPPRIDTESADHLRLLKSFFGDEGGVHSASMP
                     EAMWQGMLRAQCTWDAAMAWNAAEALRKAKDPNAVMVVLIGSGHVAYGLGAERQAKLW
                     FDGGIASVIPIPVLDEKDRPVKVRASYADFLWGVPQETDPAFPVLGLSTRDPKDGSAG
                     WPVINVAKDSVAEAAGFKVGDVLLAMDGTPLGKKGLFNRLMSEKRWSDTASYEVKRGE
                     EKVTLVAKFVRKPKEAPKAEGPAK"
     gene            complement(4148..5047)
                     /locus_tag="PLP50_15215"
     CDS             complement(4148..5047)
                     /locus_tag="PLP50_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA52943.1"
                     /translation="MTPRPPGHGAAVVTAAFVTFLWSTSWVLIKVGLAADLPPLPFAG
                     LRYAFAALCLLPVALATAGRRSLLAALDRRSLRSLSALGLLYIAIAQGGQFAALALLP
                     ASAVSLLLSLTPVAVALGSGAFLGERPTPLQAAGTTVAVSGAALYFLPAGAAVPSAAG
                     LAAGLVALLSNAGSALLGRHVNRDTHLPPLLVTFVSMSIGATVLLGAGVAVQGWPALG
                     PREGAIVAWLAVVNTAFAFTLWNRSLRVLTAVESSVLNGLMLPQIALLAVLFLGERLS
                     GRQVVALVLAAAGALAVQIRRPG"
     gene            complement(5154..5357)
                     /locus_tag="PLP50_15220"
     CDS             complement(5154..5357)
                     /locus_tag="PLP50_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52944.1"
                     /translation="MARPNYSHSKRQREIAKKQKREEKMARKLEKKNTVPGAPSDENL
                     EVLEGGEGAVPDASPSGEPEPAA"
     gene            complement(5469..>7088)
                     /locus_tag="PLP50_15225"
     CDS             complement(5469..>7088)
                     /locus_tag="PLP50_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPA52945.1"
                     /translation="FFAFLHVYEPHAPYAAPEPFRSRFADPYDGEVAAADAAVGRLVA
                     KLTALGLWEKSLVVFLSDHGEGLGEHGENEHGVFLYRESLQVPLLVKLPKGARGGTSV
                     AAPVQLSDVFRTVVEAAGLKGAPIPAGLVNLVDLAYGDAAPERRIYAETFFPRIRFGW
                     SELAALLDGSWHYVAAPKPEFYDLEADPGSTRNLAGEKPGPFRSFVVETEKLRSPFRA
                     PADVDPEHAKKLASLGYLSMTTAGGSEARPDPKDEIGSLERLKEGLGHLQADRPAEAV
                     VALQQLLERNPRVLDAWELLAQALARLGRPDEALAAIRKTVELSPPDRTSALLTVASF
                     CLQIGRPDEALAQARVARDLGDRNADEVISRAELARGNVDGAEAAARLALEGSPSTRP
                     SALFALARVAALRGDVRGALARTEEVRAAVGGRTEIASLQGFHALRGDLLARLGRTAE
                     AERELLTEIRLFPQAVEARVALAATYAATGRRAQALRTIDEMVRLLPRPDVYATGVRA
                     LRAFEEPEAAGRLAAAARARFPRDARFDRGG"
BASE COUNT          973 a         2501 c         2694 g          920 t
ORIGIN      
        1 tcgtctcgac gcccgtcacc cagctcgcgc cgcggacggc gtggatccac tcgccggccg
       61 ttccggtgcc gaggacctgg agctttccgc tgccgaggcg gccgaccgtg tcgaggttca
      121 cggcggcgag ggtcttcttc gcgtcgcgcg acgacggccc ggcgacccag cgcatcgagc
      181 cgaggcgccc cgcctcctcg cccgtgacgg cgaggaagac gaccgagcgg cggggcttct
      241 cgtccttcat cgcgcgcgcc agctcgagga gcatcgcgac gccgctcgcg ttgtcgtcgg
      301 cgccgggatg gacctgcccg gcgaactcgc tccgcgcgtc cggccagccg cgaccgaggt
      361 ggtcgtagtg cgcgaccacg acgaccgact cctccttcca cgccgggtct gagccgggga
      421 ggaggccgac gacgttcgcc acgtcggccg gcgcgccgct cgccgtcttc tcgacgcgga
      481 agcgctggaa gaacgtccct tcgtcacccg cgggggaaag gcccgcggcc ttcattccgg
      541 cggcgacgta ttccgcggcg ctcctctcgc cggccgtccc gaggccgcgc ccctcgagcg
      601 caggggccgc gagggccgcg acgtgcgcgg cgaggcgcgc ctgcgagaag gcgggcggga
      661 gctcggcgag cgccttgcgg ctctccggcg cgagcgccgg caacggcgcg gcgcgcctct
      721 ccgccgggcg gaggtcggcg cggagcggag agtcgctctc gggccactgg cccttgagga
      781 cgttcgcagg ctccgtcccc tcgaagccga cgaacgaata cttgccgtag tgggggagct
      841 tccggccgag gccggggagc gccgcgagcg gctcgccgaa gagccatccg gcggccttgc
      901 cgggatcgcc cgggtggcgg gcgacgaacg cgaacgtgtg gccggcgagc gcggccttct
      961 cgccgagggc gacgagctcc gtcgcgccga ccgcgagccc ctcctgcttc gcgaagagcg
     1021 aggcgaagcg gttcgccttc ccgacgaccc agaccgcgcg gtcgcccggg agcgaggaga
     1081 cctcgctctc cgccttcacc tcgacgtcgt gcgtcggtgt cgtccagccc gagaggagcg
     1141 cgcgccaggc ggcgtcttcc tccgcggaga ggcccgcggg gaggatcgcg aggaccttcg
     1201 gctcgccgaa gagctgtccg atcgagggag ggatctcgcg cgggtcgagc ctgcggaaga
     1261 ggtcgaactg cgggtccgcg gcgagcgcaa gcggctcggc gtcggttgcg acctcgaagc
     1321 gcgcctcgcg cccgtcgagc gggacgacct tccggatcgt ccccttctcc gtgaccaccg
     1381 cgacggggac ctcgagcggg aacgggtcgt cctcctgcgt ctgccggagc gatccgccga
     1441 cggtccagcc tgagcccgtc ttcgcgaccg tcgcggcccc gagcgcaagc gtggcggcgc
     1501 cggggcgcgt cacccacggg tcgaagaggg ccgagagggg cctgccggag acggcctcgg
     1561 ccgtcctccg gaagtcgtcg aacgtcgcgc gcctccctcg gaagtcggcg tagaaccgcg
     1621 cgaggacctt tcggaaggcc gcgtcgccga ggtggcgcct caccatgtgg aagcccatga
     1681 gcgccttccc gtagccgacc gcctccgtcg ccgcgctctc gcgcccgcgg aacgcggtca
     1741 gcgggaagtc gcgtccgtcg cgaacgtagc tccggtactt ctggagtgtc gctcggcggt
     1801 actcctcccc cttgccccgc tgctcctgga tcaggtggtc ggcgaggtag gccgtcagcc
     1861 cctcgcacca gttccccttc gcgtagtcga cgaagacgga gttcccccac cagttgtgga
     1921 ggatctcgtg cgggtaggac gacgccagga tgaaggggaa gcggatcacc tgcggcccga
     1981 ggagcgtgaa cgaggccatc ccgtagccgg tctcccagaa gttctcgacg agcgcgaact
     2041 tcccgtacgg atacgggccg atcaggccgc ggtacatctc gaggtactgc gccgtcgccg
     2101 cgaggtactt cgcagcgagc gcgtcgtcct tcgtccggag gaacgcgagc gtctcgacgt
     2161 cgcctgcgcg gtcgcggtag acgacgagag gcccgccgac gaggtaggcc tcgtcgaccg
     2221 gccccttcga gtcccaccgc gcgagtccgt cggcgccccg ggacgttccg tccccctgcg
     2281 agacgagctg ccagccgtcg gggaccttcg ccgtcatccg gaagctcgcg agcgcctcgc
     2341 cgaagacggg gacccagaac gtcgagccgg cgaggtagat cccctcgggg ccgatcgtcc
     2401 ccggcgtctc gcggaagccg cgctggtact cctccttcgg ggccgtcagc tcgttgcgga
     2461 cgatcccctc gtaggtgagc gtcagcgtcg ccggggcgcc cgaggcccgg taccgcttca
     2521 gcgtcacgcg ccgcgacggc tccgtcgccg cggcgttgtt cccgaagaag gccgcgacgt
     2581 cgccgagcgg gacctccttc agagcggggg acgacctcgt caccttcagc gcgccgttca
     2641 ggaggagctc cggcttcgcc cccggaggga gcgaaacctc cgccgcgacg cgcaggaagc
     2701 cggtcgcggg gacgaaggag acgtcgagct cgtggtggac cgccgcgagc ggggcagacg
     2761 gccccggctg ggcgagcccc ggcgacgcga agagcgccgc cgacgcgagg aggaccgccc
     2821 cggggagcgc cgcgcgcgcg cgagtcactt cgccggccct tcggcctttg gagcctcctt
     2881 cggcttgcgg acgaacttcg cgacgagcgt gaccttctcc tcgccccgct tcacctcgta
     2941 cgaggcggtg tcgctccagc gcttctccga catgagccgg ttgaagagcc ccttcttgcc
     3001 gagcggcgtg ccgtccatcg cgaggaggac gtctcccacc ttgaaaccgg cggcctcggc
     3061 gacggagtcc tttgcgacgt tgatcacggg ccagcctgcc gagccgtcct tcgggtcgcg
     3121 ggtcgagagg ccgaggaccg ggaaggcggg gtcggtctcc tgcgggacgc cccagaggaa
     3181 gtcggcgtac gaggcgcgga ccttcacggg acggtccttc tcgtcgagga cggggatcgg
     3241 gatgaccgac gcgatcccgc cgtcgaacca gagcttcgcc tgccgctcgg ccccgaggcc
     3301 gtaggcgacg tgcccggagc cgatcaggac gaccatgacg gcgttcgggt ccttcgcctt
     3361 ccggagcgcc tcggcggcgt tccaggccat cgccgcgtcc cacgtgcact gcgcgcggag
     3421 catcccctgc cacatcgcct cgggcatcga ggccgagtgc acaccgcctt cgtcgccgaa
     3481 gaacgacttg aggaggcgca ggtggtcggc gctctcggtg tcgatccgcg gcgggaggag
     3541 ggacttctgc tcgacggtca ggccgtcgaa ccccttcttc cgcacgtcgg agacgagctc
     3601 ccgcgggacg ttcaccgcgt ggagcgtgac cttcgcctcg cggcagaaga ggaagacgtc
     3661 gcggtagtag tcccagtggt agccccagct ccggtaccag tcggatcgct cgacgaactc
     3721 cgcctcggag agcgagccgg cgttccagag gtcgagcgcg gcctgctccg tggccgggta
     3781 catctcgagg ccgacgagga ccttccgtcc cgcgcggacg agctcccgga tcacctggag
     3841 ctgcacccgg tggaactcgg cgttcgtgtg gctctccccg aggaagacga gccgcgactt
     3901 cgcgagccgc gtggcgacct cggcggggga gatcgcggcg ccggtgcgcg cgtcgaggac
     3961 gacgtcgagc gacggggtga gggtcttctc cctccgcgcg gggtcgccga gcgggaggtc
     4021 gagtgcggct tcgtcggcgt agagggacgt ggcggcgaag gcgaggagga gggcggtgca
     4081 gagggctcgt ctcatggcgg cgggacgatc ctaaaccgcc cgggcgggcg ggcgcgcggg
     4141 ccgcccgtca gccggggcgc cgaatctgga ccgcgagcgc ccccgccgcg gcgaggacga
     4201 gggccacgac ctgccggccg gagaggcgct cgccgaggaa gaggacggcg agcagggcga
     4261 tctgcgggag catcagcccg ttcaggacgc tcgactcgac ggccgtcagg acgcgcaggg
     4321 agcggttcca gagcgtgaag gcgaacgcgg tgttcacgac cgccagccag gcgacgatcg
     4381 ccccctcccg cgggccgagg gcgggccatc cctgcacggc gacgccggcg ccgaggagga
     4441 ccgtcgcgcc gatcgacatg ctcacgaacg tgacgaggag cggcggcagg tgggtgtcgc
     4501 ggttcacgtg ccggccgagg agggcggagc cggcgttcga gaggagggcg acgagaccgg
     4561 cggcgaggcc ggccgccgac gggaccgcgg ctcccgccgg gaggaaatag agggcggccc
     4621 cggagacggc gacggtcgtc cccgcggcct ggagcggagt cggacgctcc ccgaggaagg
     4681 ctccgctgcc gagggcgacg gcgacgggcg tcaggctcag gaggaggctg acggcgctcg
     4741 ccggaagaag cgcgagcgcg gcgaactgcc ccccctgcgc gatcgcgatg tagaggaggc
     4801 cgagggccga gaggctccgc aaggagcgcc ggtcgagcgc ggcgaggagg ctccggcgcc
     4861 ctgccgtcgc gagcgcgacc ggcaggaggc agagggcggc gaaggcatag cgaaggccgg
     4921 cgaacggaag cggcgggagg tcggcggcga ggccgacctt gatgaggacc caggaggtcg
     4981 accagaggaa cgtgacgaag gccgccgtga cgacggcggc accgtgtccc gggggacggg
     5041 gagtcacggg aagggcatcc cgtgatggaa gcggtccggc gcgcgtccgg agtccggaac
     5101 ggcgaagggc cgccccgagg ggcggccctt gcgattcgga gtcggcgatc gcgtcacgcg
     5161 gcgggttctg gctcgccgga gggagaagcg tcgggcacgg cgccctcgcc cccctcgagg
     5221 acctcgaggt tctcgtccga gggagcgccg gggacggtgt tcttcttctc gagcttccgc
     5281 gccatcttct cttcgcgctt ctgcttcttc gcgatctcgc gttgccgctt gctgtgtgaa
     5341 tagttgggac gggccaaatc cccacctcct gaacgtggcg tcgcatcctg gggtgagctg
     5401 cttggcagaa gtagcgcccc gaactgctgc gcggaggata gcaggcccgg accgccccgc
     5461 gtccggcgtc agcctccgcg gtcgaaccgg gcgtcccggg ggaagcgagc gcgtgccgcg
     5521 gcggccaggc gacccgccgc ttccggctcc tcgaacgcgc gcagggcgcg gacgccggtc
     5581 gcgtagacgt cgggccgcgg caggagccgg accatctcgt cgatcgtccg gagcgcctgg
     5641 gccctgcgcc ccgtcgcggc atacgtcgcc gcgagggcga cccgggcctc gaccgcctga
     5701 gggaagagac gtatctccgt gagcagctcc cgctcggcct ccgcagtgcg cccgagccgg
     5761 gcgaggaggt cgcctcgaag cgcgtggaag ccctggagcg aggcgatctc ggtccgccct
     5821 ccgacggcgg cgcggacctc ctcggtccgc gcgagggcgc cgcgaacgtc accccggagc
     5881 gccgcgacgc gggcgagggc gaagagcgcg ctcgggcggg tcgaagggct gccttccagg
     5941 gcgaggcggg cggcggcctc ggcgccgtcc acgttcccgc gggcgagctc ggcgcgggag
     6001 atcacctcgt cggcgttccg gtccccgagg tcccgggcga cgcgggcctg ggcgagcgcc
     6061 tcgtcgggcc ggccgatctg gaggcagaag ctcgccacgg tcaggagcgc gctcgtccgg
     6121 tcgggcggcg agagctccac cgtcttccgg atcgcggcga gcgcctcgtc ggggcggccg
     6181 aggcgcgcga gcgcctgcgc gaggagctcc caggcgtcga ggacccgcgg gttcctctcg
     6241 aggagctgct ggagcgcgac gaccgcttcc gcggggcggt cggcctggag atggccgagc
     6301 ccctccttca gcctctcgag gctcccgatc tcgtccttcg ggtcggggcg cgcctcggac
     6361 ccgcccgccg tcgtcatgct gaggtagccg agcgaggcga gcttcttcgc gtgctccggg
     6421 tcgacgtcgg ccggggcgcg gaacggcgag cggagcttct ccgtctcgac gacgaacgag
     6481 cggaacgggc ccggcttctc cccggcgagg ttccgcgtcg agcccgggtc ggcctcgagg
     6541 tcgtagaact ccggcttcgg cgccgccacg tagtgccacg agccgtcgag gagcgccgcg
     6601 agctcgctcc agccgaagcg gatccggggg aagaacgtct cggcgtagat ccggcgctcc
     6661 ggcgccgcgt cgccgtacgc gagatcgacg aggttcacga gacccgcggg gatcggcgcc
     6721 cccttcagcc cggcggcctc gacgaccgtc cggaagacgt cgctgagctg gacgggggcg
     6781 gccacgctcg tgccgccgcg ggcccccttc gggagcttca cgaggagcgg cacctggagc
     6841 gactcgcggt agaggaagac gccgtgctcg ttctccccgt gctcgccgag cccctcgccg
     6901 tggtccgaga ggaagacgac gaggctcttc tcccagaggc cgagcgccgt cagcttcgcg
     6961 acgagccggc cgacggccgc gtcggccgcc gccacctctc cgtcgtacgg gtcggcgaag
     7021 cggctccgga acggctcggg cgccgcgtac ggcgcgtgcg gctcgtagac gtggaggaac
     7081 gcgaagaa
//
LOCUS       DAOONL010000154         7036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12662_length_7036_cov_15.435324, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000154 DAOONL010000000
VERSION     DAOONL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12662_length_7036_cov_15.435324"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1990
                     /locus_tag="PLP50_15230"
     CDS             545..1990
                     /locus_tag="PLP50_15230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPA52946.1"
                     /translation="MDAMLNVKRRLLLACYVVADLCVVALAFGVVYAVTTGVTSASDV
                     RSLLATRVQLSDVAHALLFAFAWHLCFRTQGLYRSHRIGWLIAEAWAVTRATALGTLV
                     FGSLSLFLSVSFLSRSFLLLFFGLALAGTLLLRTAVRFFLGKVRQRGRNLRHLVIVGC
                     GPRALFLGAEIRKRSDLGYHLLGYIDDIPPTGPSTSDGETHLGSLEAVEEILASLPAD
                     EIIIALPVRSQYRTIARIIAAAEDRGLMVRIPTSLFELRLSKATVDHLDDLPMITLQP
                     PMSSSDILVVKRLLDIVVSFTALVLLSPLFLVIAALIRLDSRGSVLFFQERVGQGMKR
                     FRIVKFRTMVPEAEARMKELEASNEVAGAAFKMKNDPRVTRLGRVLRKLSLDELPQFW
                     NVLKGDMSLVGPRPLPVRDFLRFEKRTQRRRFSVKPGLTCIWQVNGRHEIGFDEWMDL
                     DLEYIDNWSLGLDLEIILKTLPAVLRGTGAN"
     gene            2010..2744
                     /locus_tag="PLP50_15235"
     CDS             2010..2744
                     /locus_tag="PLP50_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPA52947.1"
                     /translation="MRAETFALLLAAAVLGTGSLGCTSAGATARQADSVASARPAAAG
                     PAAPPPEYRLQAGDVLEIKHFYTPELNETVSIRPDGRISLPIAGEVLAEGQTPAGLRD
                     VLSERYARTLRDQEVAVVVKEFAGRRIYVGGEVLTPGVIRPPGTVTALQAILEAGGVK
                     SSARLENVVILRNQGTTEPLFMTLDLRGGLERGASRNDVALLPSDIVFVPKTRVAHLA
                     QFVDVYFKQLLPIPLNLSYILGGWAP"
     gene            complement(2866..4077)
                     /locus_tag="PLP50_15240"
     CDS             complement(2866..4077)
                     /locus_tag="PLP50_15240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA52948.1"
                     /translation="MREASALHPRLRRPRAYLNWLRANYELLTRAGEIRARPLKLTFD
                     PTNYCQLRCPLCPTGARVQDRDRGRAQRHVFEHLMDEVGDYVFLIDFFNWGEPLLNEY
                     AEELVSMASRRGILTFLSSNLSIPMTDARVGRLVDSGLSQLIVSLDGATAETYGTYRR
                     QGRFDLVIENMKRVLAARRERGVTHPRVNWQFLVFRFNEHEIGTVRHMAEDLGVDSVT
                     FRAPFLDEGRVPLSEKDRSAVAGWAPTLVEFNRYDECSPAHTVVPPRPRCGWHYMSSA
                     INWDGSVAPCCTVFEKRDDFGQLSGTGGDYMAVVNNDRYRAVRNSFAGRPSKAEGLVC
                     LECPTPFLMDYHSHLNRQILLYSSASLLDALRHPVRRFRARRTRVEVLHPGEPVAGPL
                     DADVDAATLRT"
     gene            4340..5020
                     /locus_tag="PLP50_15245"
                     /pseudo
     CDS             4340..5020
                     /locus_tag="PLP50_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
     gene            complement(5140..6396)
                     /locus_tag="PLP50_15250"
     CDS             complement(5140..6396)
                     /locus_tag="PLP50_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52949.1"
                     /translation="MRRARLPLLAAALLLPARPAPADAPARSQWVLATAKATGQGGEQ
                     FVSSLRIVNPGAETATVKVTYLAQSLIDGSNAASGDNTGAATALLTVAGGETLAVEDV
                     LGTLFGAGLAPFGVPAGGLHVTSDRPVSVLSRTFVANARSSTGVPGTYGFSIPAQVES
                     QAVSPGDTAFVSYVAASPDARSGFRSNFIMLNTSGSPAVVNVDLRKGDGTSVGSRDYS
                     LGKRSAAQAGNLSGAFGYAGPDTNLTAVVTVKSGGPVVVGCSIIDNAISSIAYAPPSK
                     VTLPNDGVFGLALEDVPYNVSSARLDILDGLPVFLGGAVVVECRPSDPDPDARDPRYV
                     TFLGAGEKSGYPPNTTFTKEADGSFTVSGEDELLAIAGTIRNEVDGAVSGSLTFTTTP
                     GYARCPGQTKTYEFRGVRGSSRPSAD"
     gene            complement(6414..6680)
                     /locus_tag="PLP50_15255"
     CDS             complement(6414..6680)
                     /locus_tag="PLP50_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPA52950.1"
                     /translation="MNVLDSLRRFLANELRVADAATMDPDLALVQSGAIDSIELMQVV
                     GFLEKEFGIEVDDTEILPKNLRSLSRIVAFVEGKRAGASPGSAT"
     gene            complement(6677..>7036)
                     /locus_tag="PLP50_15260"
     CDS             complement(6677..>7036)
                     /locus_tag="PLP50_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52951.1"
                     /translation="RVELGEVESALLRAPGVTEAAVVAVERDGPGGCETELVAFVSGE
                     GPDPGRERDLRRHLAAHVLKYMVPAEIRFLREFPATSSGKVDRQTLKALAGGPESASS
                     AAPASQTPSGRSPGETT"
BASE COUNT         1030 a         2400 c         2513 g         1093 t
ORIGIN      
        1 tcgcgcggcc ggaagtgacg ggacgctcgc cggaggcgag ggggcgcttc tggcgggaga
       61 ccgcgaatcg gaatgtccga ttccgccgat ccccggctgc gcgcgaccgg aggcggcctg
      121 ttaggcattg ccgcgcaacc tgagttacag tcgcaactga actaaccggg caagaggacg
      181 ggggaacgaa ttcgaggatg gagatgtgct caggagcgat cgtcggcccg actgtgacca
      241 ggcgctccgg cctggaagcc ggattgcgcg tcggaccacg aaacgtcgcg cggggcagga
      301 ggccccagac cgcttcgacg agtccattcg gtgcgtcgtg ccatcggaac gccctgtttc
      361 cgggtcacgt ggcgacctcg aatcgaggcg tcatgccggt cgaagggtcg tctcgggtgg
      421 accctgtcgg attccaggag tcgaggtcga gcggcgtcgg gctcgaccct tccggcccgc
      481 cgggaccggc cgccgggacc cgccgccgcc gtgagcgggc gcggggagcg gccgactctc
      541 cgggatggac gcgatgctga acgtcaagcg gaggctgctc ctcgcgtgct acgtcgtcgc
      601 cgacctgtgc gtcgtggcct tggccttcgg cgtcgtctac gccgtcacca ctggggtgac
      661 gagcgcgagc gacgtccgct ccctgctcgc cacccgcgtc cagctctccg acgtcgcaca
      721 cgcgctgctc ttcgccttcg cctggcatct ctgcttccgc acgcaggggc tctaccgctc
      781 tcaccggatc gggtggctga tcgcggaggc gtgggccgtc acgcgcgcga ccgccctcgg
      841 gaccctcgtt ttcgggagcc tctcgctctt cctctcggtc tcgttcctct cgcgatcgtt
      901 cctgctcctg ttcttcggac tcgccctggc cggaacgctc ctccttcgga cggccgtccg
      961 gttcttcctg gggaaggttc gccagagggg gcgcaacctc cggcacctcg tgatcgtggg
     1021 ctgcgggccg cgcgcgctgt tcctcggggc cgagatccgg aagcgctccg atctcggcta
     1081 tcacctgctc gggtacatcg acgacatccc gccgacgggc ccctcgacca gtgacgggga
     1141 gacgcacctg ggttccctgg aggcggtgga ggagatcctc gcgagccttc cggccgacga
     1201 gatcatcatc gcgcttccgg tccggtccca gtacaggacg atcgcacgga tcatcgcggc
     1261 ggccgaggac cgcgggctca tggtgaggat cccgacgagc ctgttcgagc tcaggctttc
     1321 caaggcgacc gtcgatcacc tcgacgacct gccgatgatc accctccagc cgccgatgtc
     1381 ctcctcggac atcctcgtcg tcaagcggtt gctggacatc gtcgtctcct tcaccgccct
     1441 ggtcctcctc tccccgctct ttctcgtcat cgccgccctg atccgtctcg acagccgcgg
     1501 gagcgtcctc ttcttccagg agagggtggg acaggggatg aagcggttcc gcatcgtgaa
     1561 gttccggacg atggtcccgg aggccgaggc caggatgaag gagctggagg cgagcaacga
     1621 ggtggccggc gcggccttca agatgaagaa cgacccgcgc gtcacgcggc tcgggcgggt
     1681 gctgaggaag ctgagcctgg acgagctgcc gcagttctgg aacgtcctca agggcgacat
     1741 gagcctcgtg gggccccgcc cgcttcccgt ccgcgacttc ctgaggttcg agaagcggac
     1801 ccagaggaga cgcttcagcg tgaagccggg tctgacgtgc atctggcagg tcaacggccg
     1861 gcacgagatc ggcttcgacg agtggatgga cctcgacctc gagtacatcg acaactggtc
     1921 gctcgggctc gacctcgaga tcatcctgaa gacgctgccc gccgtcctgc gcggcaccgg
     1981 cgcgaactga gagagaaacg accgacgaca tgagagcaga gaccttcgcc ctcctcctcg
     2041 ccgccgcggt cctcgggacc gggtccctcg gctgcacgtc ggccggagcc accgcccggc
     2101 aggccgactc cgtcgcctcg gcccgcccgg ccgcggccgg gccggcggcc ccgccgcccg
     2161 agtaccgtct ccaggccggg gacgtcctcg agatcaagca cttctacacg ccggagctga
     2221 acgagaccgt ctcgatccga cccgacggcc ggatctcgct cccgatcgcg ggagaggtgc
     2281 tggcggaggg gcagacgccg gcgggcctgc gcgacgtcct ctccgagcgg tacgcacgga
     2341 cgctccgcga ccaggaggtc gccgtcgtcg tgaaggagtt cgcgggacgt cggatctacg
     2401 tcggcggcga ggtcctcacc cccggcgtca tccgcccgcc gggaaccgtc acggcgctgc
     2461 aggcgatcct ggaggccggg ggggtcaaga gctcggcccg actcgagaac gtcgtcatcc
     2521 tgaggaacca ggggacgacg gagccgctct tcatgacgct ggacctccgg ggcggcctgg
     2581 agcggggagc gagccgcaac gacgtggctc tcctcccgtc ggacatcgtc ttcgtcccga
     2641 agacgagagt tgctcatctg gcccagttcg tcgacgtcta cttcaagcag ctcctcccga
     2701 tccctctgaa cctgagctac atcctcggcg ggtgggcgcc ctgacgagcc ggcaggccgg
     2761 tcgcccgggc cggaggaggg aggcccgttc cgacgagggg tcgtcgcgcc ccgccggctc
     2821 ccgcccgctt cggcggggca ggtccggcgg ggcggcgtcg gagtcctagg tccggagcgt
     2881 cgcggcgtcg acgtcggcgt cgagagggcc ggcgacgggt tcgccgggat gaagcacctc
     2941 cacccgagtc cgccgcgccc ggaagcggcg aacgggatgg cgaagggcgt ccagcaggct
     3001 cgccgacgaa tagagcagga tctgtcgatt caggtgcgag tgatagtcca tcaggaaggg
     3061 cgtggggcac tcgaggcaga cgaggccttc ggccttcgaa ggccgacccg cgaagctgtt
     3121 tcgtacggct cggtaccggt cgttgttgac gacggccatg tagtcgccgc ccgttcccga
     3181 cagctggccg aagtcgtccc gtttctcgaa caccgtgcag cagggggcca cgctcccgtc
     3241 ccagttgatc gcggaggaca tgtagtgcca cccgcagcgc ggacgtggcg ggacgacggt
     3301 gtgcgccggg ctgcactcgt cgtagcggtt gaactcgacg agcgtgggcg cccatcccgc
     3361 gaccgccgac ctgtccttct ccgagagcgg cacgcgcccc tcgtcgagga aaggcgcccg
     3421 gaaggtgacg ctgtcgacgc ccaggtcctc cgccatgtgg cggacggtgc cgatctcgtg
     3481 ctcgttgaac cggaagacga ggaactgcca gttcactctc gggtgcgtga cgccgcgttc
     3541 ccggcgtgcc gcgagcaccc gcttcatgtt ctcgatcacg aggtcgaacc gtccctgccg
     3601 ccgatacgtg ccgtacgtct cggcggtcgc gccgtcgagg gagacgatga gctggctcag
     3661 gccgctgtcg acgagccgcc ccacgcgcgc gtcggtcatc gggatgctca ggttcgagct
     3721 gaggaacgtc aggatcccgc gtcggctcgc catcgagacg agctcctcgg catattcgtt
     3781 cagcagcggc tcgccccagt tgaagaagtc gatcaggaag acgtagtccc ccacctcgtc
     3841 catcaggtgc tcgaagacgt gcctctgcgc ccggccacga tcgcggtcct gaacgcgggc
     3901 ccccgtgggg cagagcggac agcgaagctg acagtagttc gtcgggtcga acgtcagctt
     3961 caggggccgg gcgcggatct ctccggcacg ggtcaggagc tcgtagttgg ccctcagcca
     4021 gttcaggtag gcccgggggc gtcgcaggcg aggatgcaga gcggaggctt cccgcatccg
     4081 gtctcgcaag cgtgtccacg aggaacccgg gctcgagcgc tcggcggccc cgccggtctc
     4141 gcgagcttcg ttcacggatg gagggctgcg atcaagcgat gctcgatgtc gaggagtcgt
     4201 ccctggtgat gcacatgtgt gacaataggg gcatcgagac gcgttcgcaa ccgatgaggc
     4261 cggatccgca gtccccagct gccgaggcaa ggggcgggcc ttcggctcgc cgcggagtct
     4321 cgccggaatc ccgacgccca tgagctcgga gcagcgtccg gtccgccttc tgcccgtaga
     4381 gcagagctcg tccgaggacg aggtgcgcct cggggtgatc tcggctgccc gcgcgatgaa
     4441 cgccctcggc ctcaaccggg gcaccgccgg caacgtcagc gcgagatggc gcgacgggtt
     4501 tctcgtgact ccgaccggga tggcctacga cgagacgttg ccggacgaca tcgtcttcgt
     4561 gcgccgcgac ggctcctggc gcggggcgcg caggccgtct tcggaatggc gcttccaccg
     4621 ggacatttac gaagcccgcg aagaggccgg cgccatcctg cacgcgcatt cgtcgttcgc
     4681 caccgcgctc gcctgcctgc gcagcgggat cccgccgttc cactacatga tcgcgcgctt
     4741 cggcggcgga tcgatccgct gcgccgacta cgccactttc gggacgcagg agctctcgag
     4801 gaacgtcgtc gacgccctgg aagggcgccg cgcctgcctg ctggcgaatc acggcatgct
     4861 cgtcttcggc cgcggtctgc gccatgcgct ggacctcgcc gtggagctcg agacgctctg
     4921 cgagcagtac tggcgagccc gacagatcgg cgagccggtc ctcctcgcgg acgccgagat
     4981 cggcagggtg ctggagaggt tcgcggacta cggccagcag gagacctccg gggccgactc
     5041 cgaccagggg agtccgggcc gcgccgagcg gcgctggagg ccccgacccg gagcggacgg
     5101 cgtgccttcg accggcgccg gagggacgag cccgagcggt cagtccgcgg aggggcggga
     5161 cgaaccccgg actccccgga actcgtaggt cttcgtctgc ccgggacagc gggcgtagcc
     5221 gggcgtggtc gtgaaggtga gactcccgga gaccgcgccg tccacctcgt tccggatggt
     5281 gccggcgatg gcgaggagct cgtcctcgcc cgagacggtg aagctgccgt ccgcttcctt
     5341 cgtgaacgtg gtgttcggcg ggtatccgct cttctctccg gctccgagga aggtgacgta
     5401 ccgcggatcg cgcgcgtccg ggtccgggtc ggacggccgg cactcgacga cgaccgcccc
     5461 tccgaggaag accgggagcc cgtccaggat gtcgaggcgg gcgctcgaga cgttgtacgg
     5521 aacgtcctcg agcgcgagcc cgaagacgcc gtcgttgggc agggtgacct tggagggcgg
     5581 tgcgtacgcg atggaggaga tggcattgtc gatgatcgag cagcccacga cgaccgggcc
     5641 accgctcttc acggtgacga cggcggtgag gttcgtgtcg ggtccggcgt agccgaaggc
     5701 cccggagagg ttgcccgcct gggcggcgga gcgcttcccg aggctgtagt ccctgcttcc
     5761 gaccgacgtc ccgtcgccct ttcgaaggtc gacgttcacc acggccgggc tgcccgacgt
     5821 gttgagcatg atgaagttcg agcggaagcc ggaccgcgcg tccggagagg cggcgacgta
     5881 cgagacgaag gcggtgtcgc ccggcgagac ggcctggctc tcgacctggg ccggaatcga
     5941 gaagccgtac gtgcccggca cgccggtcga gctcctggcg ttggcgacga aggtccggga
     6001 gagcaccgac acgggccggt cggaggtcac gtggagcccg ccggcgggga ccccgaaggg
     6061 ggcgaggccg gctccgaaga gcgtgccgag gacgtcctcg acggccagag tctcgccccc
     6121 ggcgacggtc aggagagccg tcgcggcgcc ggtgttgtcg ccggaggccg cgttcgagcc
     6181 gtcgatgagg gactgggcga ggtaggtcac cttcacggtc gcggtctcgg cgccggggtt
     6241 gacgatccgg agcgagctga cgaactgctc cccgccctgg cccgttgcct tcgccgtggc
     6301 caggacccac tggcttcgcg ccggcgcgtc cgcgggtgcg ggacgagccg ggaggaggag
     6361 cgcggcggcg agcagcggga ggcgggcgcg tcgcacgttc aggctccgca ggctcaggtc
     6421 gcggagcccg ggctcgctcc cgcgcgcttg ccctcgacga acgccacgat tcgcgagagg
     6481 ctccggaggt tcttcggcag gatctccgtg tcgtcgacct cgatgccgaa ctccttctcg
     6541 aggaagccga cgacctgcat cagctcgatc gagtcgatcg ctccgctctg aaccagggcg
     6601 agatccgggt ccatcgtcgc cgcgtcggcg acgcggagct cgttcgcgag gaaccgcctg
     6661 agggaatcga gtacgttcat gtcgtctctc ccgggcttct gcccgacggg gtctggctcg
     6721 ccggcgcagc gctgcttgcg ctctccggac cgccggcgag ggccttgagg gtctggcggt
     6781 cgaccttccc gctcgaggtg gcggggaact ccctgaggaa tcggatctcc gccgggacca
     6841 tgtacttgag gacgtgggcc gcgaggtggc gccggaggtc ccgttcgcgg cccgggtccg
     6901 gaccttcgcc cgagacgaac gcgacgagct ccgtctcgca gcctcctggg ccgtcccgtt
     6961 cgacggcgac gacggcggcc tccgtcactc ccggggcgcg caggagggcc gactcgacct
     7021 cgccgagctc cacccg
//
LOCUS       DAOONL010000155         6993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12758_length_6993_cov_17.796916, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000155 DAOONL010000000
VERSION     DAOONL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_6993_cov_17.796916"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..190
                     /locus_tag="PLP50_15265"
     tRNA            116..190
                     /locus_tag="PLP50_15265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Val,seq:gac)
     gene            213..288
                     /locus_tag="PLP50_15270"
     tRNA            213..288
                     /locus_tag="PLP50_15270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:246..248,aa:Val,seq:cac)
     gene            359..2317
                     /gene="thrS"
                     /locus_tag="PLP50_15275"
     CDS             359..2317
                     /gene="thrS"
                     /locus_tag="PLP50_15275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPA52952.1"
                     /translation="MSANAENVVRLTLPDGSVREVTTGTTGEAVAASIGARLAKDALA
                     VKVAGKILDLKRPITESGAFEVITPKHPDALEVYRHSTAHLTAHAVKRLFPGVKIGIG
                     PAIENGYYYDFDPGRAFTPEDLERIEAEMRKIVAEDAPVERLEMSKAEAVKIFEEQGD
                     HLKVEIVSGIPEGSLSCYRQADFIDLCRGPHVPSTGKLGVFKLTHAAGAYWKGDEQNP
                     MLQRIYGAAFLSQKELDEHLLRMEQARARDHRKLGKELGLYLFHPWAPASPFFLPKGA
                     TVYTLLVDYMRSLYPKYGYTEVVTPQVYDVELFKKSGHYQNYHENMFFTVADEREYGV
                     KPMNCPGHFLMFSQQAFSYRDLPVRYADFGRLHRYERSGVTQGLTRVRTFCQDDAHLF
                     VSLEEMPAEMDRFIDLIDEVYGAFGFTDVKVALATRPEKYMGSLENWDLAEKALAAAF
                     ERRGRAYEINAGDGAFYGPKLDFQVTDAIGRAWQLGTLQVDFSNPERFDLEYTAEDGT
                     KKRPVVLHRAVLGSLERFFGILVEHTGGDFPLWLSPEQARVIPVSDKLLDYARSVAAE
                     LRAAGLRVTVDERNEKLGAKIRHGELEKVPALLVVGEKEREAGAVSLRKRHEGDLGSR
                     KLAEVVSEMKQAVAARAATWGAKTPAKK"
     gene            2372..2896
                     /gene="infC"
                     /locus_tag="PLP50_15280"
     CDS             2372..2896
                     /gene="infC"
                     /locus_tag="PLP50_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPA52953.1"
                     /translation="MPPQKNSPINEFILRYPEVRLIAADGEQLGVVTPQDALARAREV
                     ALDVVVIAEQAQPPVCKIMDFGKFTFEKKKKEHESKRKQKVTQVKEVKFRPNIDDHDY
                     DFKVKNMIRFIEEGDKVKATIQFRGREMSRLENGRKVLQRLGADLEGKAHAEGNPEML
                     GNRMHLIFAPPKKH"
     gene            2991..3194
                     /gene="rpmI"
                     /locus_tag="PLP50_15285"
     CDS             2991..3194
                     /gene="rpmI"
                     /locus_tag="PLP50_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPA52954.1"
                     /translation="MATHKRKTHRGAAKRFTITASGKFKRGNAMKRHILTKKASNRKR
                     QLAKSGLVAKADQATVRQMLPYA"
     gene            3216..3578
                     /gene="rplT"
                     /locus_tag="PLP50_15290"
     CDS             3216..3578
                     /gene="rplT"
                     /locus_tag="PLP50_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPA52955.1"
                     /translation="MPRVKRGTKRNDRRKRILALAKGYYGTKHNCFRIAELQVERSLA
                     YATRDRKQKKREFRGLWITRINAAARMNDISYSQLVAGLKKAGSDVNRKVLAEIAFQD
                     PKGFAALVSSAKSALSAA"
     gene            3591..4682
                     /gene="pheS"
                     /locus_tag="PLP50_15295"
     CDS             3591..4682
                     /gene="pheS"
                     /locus_tag="PLP50_15295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPA52956.1"
                     /translation="MSSTRPEAGGAAPVSVEEARGLLDAELAAAAGRGGLLPGDVHEL
                     KVRWLGKKQGVVTTLLARLKEVPKEEKAAFGAGVNALKQEVEARLDVLEAEASARAAA
                     LAEAAKAVDVTLPPRVPPVGRLHPLTVVRRRIEHVFRGLGYEVADGPEIETDWYNFES
                     LNFAPDHPARDSVDTFFIEGGGTGRDALLLRTHTSPVQMRTMEGRRPPIRVLCPGRVY
                     RKDDIDPRHSPVFHQMEGLVVDEGITFAHLRGTLSAFAKSFFGGRAEVRLAPTFFPFV
                     EPGVDCAVSCVFCETGCRICSYSGWIEIMGAGMVHPNVLANAGIDPERYTGFAFGMGI
                     DRIAMLLHGIPDLRLLFENDVRFLDRTWS"
     gene            4746..6812
                     /gene="pheT"
                     /locus_tag="PLP50_15300"
     CDS             4746..6812
                     /gene="pheT"
                     /locus_tag="PLP50_15300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPA52957.1"
                     /translation="MKFRLEWLGQYLPGDLPDLATLRRRLTDVGFIVEGTEGEGKDAV
                     LEVELTANRPDGMNHRGLAREAACALGRPFRDVPVPAPAEGAAAVSSLATVTIAAPEL
                     CSRYSARVVEGIRVREASARVQVRLRALGMNPISAPVDATNHVLWDLGQPLHAFDLET
                     LAKGPDGLPAIVVRLARAGETLVTLDGVERTLTPEDLVIADAERPVALAGVMGGLETA
                     ITPSTTRVLLESAHFNATAVRKTARRHGMHTDASHRFERGTDPETTVEALDRVTELLL
                     ADCGGTVARGVIDALGTPRPRQTVTLRHARLVSFLGMEVPLPRCVEILSSLAFGVREA
                     APGVLEVSVPTWRVDVEQECDLIEEVIRVVGYSSLPETLPHAYNPTWSEPTLAREERA
                     RDLFAAAGFAEACTYSFVSAEENSPFASCVDGTPPVVSNALGEPFTTMRSTPVVGLLR
                     AARHNVRRGLGDLALFEVGRSYALVEGRPVESRRAAILLRGRRGVHWAAEARPADFYD
                     GSGAVAALFAGFGAPAPAFRPAALPFLAPGRSAEVVADGKVAGWVGVLAGPLGATWDL
                     GEAVVADVELAAIPVPSPPSSVEMPSRFPGSGVDLTVTHPIALSWDTLVAAVRTGAPA
                     ELLSVEAKYLWRGAGVPEGHVKTTLTVAFGSAERSLERDEINRWRDEAARRLLAVPGA
                     AVDGIS"
BASE COUNT         1088 a         2497 c         2428 g          980 t
ORIGIN      
        1 ctccgcggtc ccggcacgtg tgccggagcg ggtcttttga gaatcagatc cgggtcgagg
       61 gtggggccga agtacctcct gcgaggtccc ctgccggtag ccctggaaag cacgtaggcg
      121 cgtagctcag tgggagagca ctaccttgac acggtagggg tcagcagttc gatcctgctc
      181 gcgcctacca tcccgctttc ctctgcgttc gcaggcgctt agctcagccg gtagagcact
      241 tccttcacac ggaaggggtc agcggttcga gtccgctagc gcctaccatc ccgcttcccc
      301 ctcctcccag aatccgatct cggaaccccg ccaccacgag atggaggagg tcgtcgccat
      361 gtccgccaac gccgaaaacg tcgtccgcct gacccttccc gacggttccg tccgggaggt
      421 gactacgggc acgacgggcg aggcggtggc ggcctccatc ggcgcccgcc tcgcgaagga
      481 cgccctcgcc gtcaaggtcg ccgggaagat cctcgacctg aagcgcccga tcaccgagtc
      541 gggggcgttc gaggtgatca ccccgaagca ccccgacgcg ctcgaggtct accgccactc
      601 gaccgcccac ctcaccgccc acgccgtcaa gcgcctcttc ccgggcgtga agatcgggat
      661 cggtccggcc atcgagaacg gctactacta cgacttcgac ccgggccggg ccttcacccc
      721 cgaggacctc gagaggatcg aggccgagat gcggaagatc gtggcggagg acgccccggt
      781 cgagcggctc gagatgtcga aggccgaggc cgtgaagatc ttcgaggagc agggcgacca
      841 cctgaaggtg gagatcgtct ccggcatccc cgagggctcg ctctcctgct accgccaggc
      901 cgacttcatc gacctctgcc gcggaccgca cgtcccctcc accgggaagc tcggcgtctt
      961 caagctgacg cacgccgccg gcgcctactg gaagggcgac gagcagaacc cgatgctcca
     1021 gcggatctac ggggccgcgt tcctctccca gaaggagctg gacgagcacc tgcttcgcat
     1081 ggagcaggcc cgggcccgcg accaccgcaa gctcggcaag gagctggggc tctacctctt
     1141 ccacccgtgg gcgccggcct cgccgttctt cctcccgaag ggggcgacgg tctacaccct
     1201 cctcgtcgac tacatgcgga gcctctaccc gaagtacggg tacaccgagg tcgtgacccc
     1261 gcaggtctac gacgtggagc tcttcaagaa gtcggggcac taccagaact accacgagaa
     1321 catgttcttc acggtggccg acgagcggga gtacggcgtc aagccgatga actgcccggg
     1381 ccacttcctg atgttctcgc agcaggcctt ctcctaccgc gacctcccgg tgaggtacgc
     1441 cgacttcggg cggctccacc gctacgagcg gtcgggcgtg acccaggggc tgacccgcgt
     1501 gcggaccttc tgccaggacg acgcgcacct cttcgtctcg ctcgaggaga tgccggcgga
     1561 gatggaccgt ttcatcgacc tgatcgacga ggtctacggc gcgttcggct tcacggacgt
     1621 gaaggtcgcg ctcgcgaccc gccccgagaa gtacatgggg tcgctcgaga actgggacct
     1681 cgccgagaag gcgctcgccg cggcgttcga gcggcgcggc cgggcgtacg agatcaacgc
     1741 cggggacggc gcgttctacg gcccgaagct cgacttccag gtcaccgacg cgatcgggcg
     1801 cgcctggcag ctcgggacgc tccaggtcga tttctcgaac ccggagcggt tcgacctcga
     1861 gtacacggcc gaggacggca cgaagaagcg gccggtggtc ctccaccggg cggtcctcgg
     1921 ctcgctcgag cgcttcttcg ggatcctcgt cgagcacacc gggggcgact tcccgctctg
     1981 gctctcgccg gagcaggcgc gcgtcatccc ggtctccgac aagctcctcg attacgcccg
     2041 ctccgtcgcg gccgagctcc gcgccgcggg cctgcgcgtg acggtcgacg agcggaacga
     2101 gaagctcggg gcgaagatcc ggcacggcga gctcgagaag gtcccggcgc tcctcgtcgt
     2161 cggcgagaag gagcgcgagg ccggggcggt gtcgctccgg aagcgccacg agggggacct
     2221 cgggtcgcgg aagctcgccg aggtcgtctc cgagatgaag caggccgtcg ccgcgcgtgc
     2281 ggcgacgtgg ggcgcgaaga cgcccgccaa gaagtaacga gtcgtcgcag caaaaggagg
     2341 aagcgccatt ttctttcgag gcggtccacg gattcccccc cagaagaact ccccgatcaa
     2401 cgagttcatc ctccgctatc ccgaagtgcg cctgatcgcc gcggacggcg agcagctcgg
     2461 ggtcgtcacc ccgcaggacg cgctcgcgag ggcgcgcgag gtggccctcg acgtcgtcgt
     2521 catcgcggag caggcccagc ctcccgtctg caagatcatg gacttcggca agttcacctt
     2581 cgagaagaag aagaaggagc acgagtcgaa gcggaagcag aaggtgacgc aggtcaagga
     2641 ggtcaagttc cgcccgaaca tcgacgacca cgactacgac ttcaaggtca agaacatgat
     2701 ccggttcatc gaggagggcg acaaggtcaa ggccacgatc cagttccgcg gccgcgagat
     2761 gtcccgcctc gagaacgggc ggaaggtcct ccagcgcctc ggcgcggacc tcgaggggaa
     2821 ggcccacgcg gaagggaacc ccgagatgct cggcaaccgg atgcacctga tcttcgcgcc
     2881 cccgaagaag cactgacctc ccgccggggg gaccccggga ccgccccgcg acgcggggac
     2941 ccgcagccct ccggctcacg aacgatccac ggagcaagga gacccggccc atggccaccc
     3001 acaagaggaa gacccatcgc ggcgccgcca agcgcttcac catcaccgcg agcgggaagt
     3061 tcaagcgcgg caacgcgatg aagcggcaca tcctgacgaa gaaggcgtcc aaccggaagc
     3121 ggcagctcgc gaagtcgggc ctcgtcgcca aggcggacca ggccaccgtc cggcagatgc
     3181 ttccgtacgc gtgagaagga agaggagata ggaccatgcc tcgcgtcaag cgcggaacga
     3241 agaggaacga ccgtcggaag aggatcctgg ccctggcgaa gggctactac ggaacgaagc
     3301 acaactgctt ccgcatcgcc gagctgcagg tcgagcgctc gctcgcctac gccacgcgcg
     3361 accgcaagca gaagaagcgc gagttccggg gcctctggat cacgcgcatc aacgccgccg
     3421 cccggatgaa cgacatcagc tactcgcagc tggtggcggg cctcaagaag gccggctcgg
     3481 acgtgaaccg caaggtcctc gccgagatcg ccttccagga tccgaagggc ttcgccgccc
     3541 tcgtctcctc cgccaagagc gccctctccg ccgcctgagg cgggagcccg gtgagcagca
     3601 ctcgccctga ggccgggggg gcggcgccgg tcagcgtcga ggaggcccgg ggcctcctcg
     3661 acgccgagct cgccgccgcg gcgggacgcg gcggcctcct gccgggtgac gtccacgagc
     3721 tgaaggtccg gtggctcggg aagaagcagg gcgtcgtcac gaccctcctc gcgcgcctga
     3781 aagaggtgcc gaaggaggag aaggccgcgt tcggcgcggg tgtgaacgcg ctgaagcagg
     3841 aggtcgaggc gcgcctcgac gtgctcgagg cggaggcctc cgcccgcgcg gccgcgctcg
     3901 cggaggcggc gaaggccgtc gacgtcacgc tgccgccgcg ggtcccgccg gtcgggcgcc
     3961 tccacccgct gacggtcgtg aggcggcgaa tcgagcacgt cttccggggc ctcggctacg
     4021 aggtcgcgga cgggccggag atcgagaccg actggtacaa cttcgagtcg ctcaacttcg
     4081 ccccggacca cccggcgcgc gactcggtcg acacgttctt catcgagggg ggcgggacgg
     4141 ggcgcgacgc gctcctcctc cggacccaca cctcgcccgt gcagatgcgg acgatggagg
     4201 ggcgcaggcc cccgatccgc gttctctgcc cggggcgcgt ctaccgcaag gacgacatcg
     4261 acccgcgcca ctctcccgtc ttccaccaga tggaggggct cgtcgtcgac gaggggatca
     4321 cgttcgcgca cctgcgcggc acgctctcgg ccttcgcgaa gagcttcttc ggcgggcgcg
     4381 ccgaggtgcg cctcgctccg acgttcttcc cgttcgtcga gccgggcgtc gactgcgcgg
     4441 tgagctgcgt cttctgcgag acggggtgcc ggatctgctc gtactccggc tggatcgaga
     4501 tcatgggggc cgggatggtc cacccgaacg tcctcgcgaa cgcggggatc gacccggagc
     4561 gctacacggg cttcgcgttc gggatgggga tcgaccggat cgcgatgctc ctccacggca
     4621 tcccggacct gcgcctcctc ttcgagaacg acgtccggtt cctcgaccgg acgtggagct
     4681 gaccgtgcgc tctgcgcccg gggaacgagg aagccgcggc cgcggcttcc cggggagctg
     4741 acccgatgaa gttccgcctc gagtggctcg ggcagtacct gcccggcgac cttcccgacc
     4801 tcgccacgct ccgccgccgc ctcacggacg tcggcttcat cgtggaaggg acggaggggg
     4861 aggggaagga cgcggtcctc gaggtcgagc tgacggcgaa ccgccccgac ggtatgaacc
     4921 accgcgggct ggcccgcgag gccgcctgcg ccctcggccg gccgttccgc gacgtccccg
     4981 tgccggcgcc ggcggaggga gcggccgcgg tgtcctccct cgcgacggtg acgatcgcgg
     5041 cgccggagct ctgctcccgg tactcggcgc gcgtcgtgga ggggatccgg gtccgtgagg
     5101 cgtcggccag ggtgcaggtg cgcctccgcg ccctcggcat gaacccgatc agcgcgcccg
     5161 tcgacgcgac gaaccacgtc ctctgggacc tcgggcagcc gctccacgcc ttcgacctcg
     5221 aaacgctcgc gaagggcccg gacgggctcc ccgcgatcgt cgtccggctc gcacgcgccg
     5281 gggagacgct cgtcaccctc gacggcgtcg agcggacgct cactccggag gatctcgtca
     5341 tcgccgacgc cgagcggccc gtcgccctcg ccggcgtcat ggggggcctc gagacggcga
     5401 tcacgccgtc cacgacgcgc gtcctcctcg agtcggccca cttcaacgcg acggcggtga
     5461 ggaagacggc ccgccgccac gggatgcaca cggacgcctc gcaccgcttc gagcggggga
     5521 ccgacccgga gacgacggtc gaggcgctcg accgcgtcac ggagctcctc ctcgccgact
     5581 gcggcggaac ggtcgcgcgc ggggtgatcg acgccctcgg gacgccgcgc ccgcggcaga
     5641 cggtgaccct gcgccacgcg cgcctcgtct cgttcctcgg gatggaggtg ccgctcccgc
     5701 gctgcgtcga gatcctctcc tccctcgcgt tcggcgtgag ggaagcggcc cccggagtcc
     5761 tcgaggtctc cgtgccgacc tggcgggtcg acgtcgagca ggagtgcgac ctgatcgagg
     5821 aggtcatccg ggtcgtcggc tattcctcgc tccccgagac gctgccgcac gcctacaacc
     5881 ccacctggtc cgagccgacc ctcgcccgcg aggagcgcgc ccgggacctc ttcgccgcgg
     5941 cgggcttcgc cgaggcgtgc acctactcgt tcgtctcggc cgaggagaac tctcctttcg
     6001 cgtcctgcgt cgacgggacg cccccggtcg tctcgaacgc gctcggggag ccgttcacga
     6061 cgatgcgttc gacgccggtg gtcgggctcc tccgggcggc ccggcacaac gtccggcgcg
     6121 ggctcggcga cctcgccctc ttcgaggtcg gccgctccta tgcgctcgtc gagggccgcc
     6181 cggtcgagtc ccggcgcgcg gcgatcctcc tgcgaggccg ccgcggcgtg cactgggcgg
     6241 ccgaggcgcg ccccgccgac ttctacgacg gctcgggcgc cgtggccgcc ctcttcgccg
     6301 ggttcggcgc gcccgccccg gcgtttcggc cggccgcgct ccccttcctc gcgccgggtc
     6361 gctcggccga ggtcgtcgcg gacgggaagg tcgccgggtg ggtcggtgtc ctcgccgggc
     6421 cgctgggcgc cacgtgggac ctcggagagg cggtcgtcgc cgacgtcgag ctcgccgcga
     6481 ttccggtgcc gtccccccca tcgtcggtgg agatgccgtc gaggttcccc ggcagcggtg
     6541 tcgacctgac ggtgacgcat ccgatcgcgc tttcgtggga cacgctcgtc gcggccgtcc
     6601 ggaccggcgc cccggccgag ctcctctcgg tcgaggcgaa atacctctgg cgcggtgccg
     6661 gcgtgcccga ggggcacgtg aagacgaccc tcaccgtggc cttcgggtcc gcggagcgct
     6721 ccctcgagcg cgacgagatc aaccgctggc gcgacgaggc cgcccggcgc ctcctcgccg
     6781 tccccggggc ggcggtggac ggaatctcct gaaggagaac cagatgagcg gaaagaagga
     6841 caaggctcct gtcgaggagc cggcttcgga aacggtctcg gagctggagc agctgctcgg
     6901 cgtgatggag gagaaggtga cccgtctcct ttccgcggtg agatcggccg ccggtgagaa
     6961 cgcccggctg ctcgcggcgc tggaggagac ggc
//
LOCUS       DAOONL010000156         6971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_12821_length_6971_cov_13.132736, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000156 DAOONL010000000
VERSION     DAOONL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12821_length_6971_cov_13.132736"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLP50_15305"
     CDS             complement(<1..758)
                     /locus_tag="PLP50_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPA52958.1"
                     /translation="MNPKEELLGKIASRSARVGIIGLGYVGLPLALVFEEAGFPVLGF
                     DVDPKKPEALHRGESYIKHIGPDRVAAAFTRGRIAATTDFARLAECDAILICVPTPLG
                     KHREPDISYIQTTAEAIAAVARPGQLVVLESTTYPGTTREEILPRLEAVGLKCGETVF
                     VAFSPEREDPGNPSFHTRNIPKVVGGIDAPSGDVAEALYAAALERVIRVSTAEVAESG
                     KLLENIFRSVNIALVNELKVVFDKMGIDVWEVIEA"
     gene            complement(768..1769)
                     /locus_tag="PLP50_15310"
     CDS             complement(768..1769)
                     /locus_tag="PLP50_15310"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPA52959.1"
                     /translation="MDCDREALAELVADPALLAAPRFKRAIPAIALAASESGLEVPAP
                     WRNDLIGAAVAGMLLKQQLDAVGSVLDAAGIPWMPIKGMDLGYRLWPGPEARPTSDLD
                     ILVPAARFAEARAFLGEAGWKGLLNGDEYECFLASEAYNWEARNDSGILLELHFRLWP
                     STPPSWAESLWENCIDAPDLGSQARRPSWPDAFVLAAVHLWQLPPPHNLVYFRDLELI
                     ARRAGESALEEAGASARRWGVSLQVCLAALYTTHLWPNPAMKHLAESLRSDLYLPERL
                     LYSRAVARGIDAASIGELWLARLLSGRKTRHGWRVVLRPLRPHSALRPRRAAAIRTA"
     gene            complement(1906..3306)
                     /locus_tag="PLP50_15315"
     CDS             complement(1906..3306)
                     /locus_tag="PLP50_15315"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA52960.1"
                     /translation="MNRTSELARLDALVERGQQGLVALWGRRRVGKTRLLLEWSRRHD
                     GLYSVADLSSAPVQRAYLADALSLRFPGFGDAAWPDWRSLLRALSREATRAGWRGPLV
                     LDELPYLVETSPSLPSVLQGWLDHEAKDARLLVAVSGSTQHMMQGLTLGAGAPLFGRT
                     VSAMPIEPMTPRALGKALGLGDALGAVRAFAAWGGIPRYWELAEPFGADLPEALDELV
                     LDPLGPLHLEPDRLLAEERPSAVSLRPLLDVIGAGAHRVSEIAGRIGQPATSLARSLA
                     RLAELGLVSRELPFGEPEKGGKRSLYRIADPFFRFWFRIVAPRRALLAVAPREARRRL
                     VREHLPRLLSETWEDLCRRAVPRLGSAVARGGTAWGPARRYWHGSGSEWDVVALSEDG
                     RRLLLGEVRWHDQPVAGEALERIHRELLAKGRPPVGDGSEEVVRAVFVPRTKERPGSR
                     LPFLVFDAGALVEAIG"
     assembly_gap    3396..3495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3581..3808)
                     /locus_tag="PLP50_15320"
     CDS             complement(3581..3808)
                     /locus_tag="PLP50_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPA52961.1"
                     /translation="MERLIAVHVEKLPEGVYLASSDDVQGLVAQGRTIAETLEIARDV
                     ARRLLEAQSERQPLPDPPPVDRFDLTLVVGG"
     gene            complement(3869..4327)
                     /locus_tag="PLP50_15325"
     CDS             complement(3869..4327)
                     /locus_tag="PLP50_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPA52962.1"
                     /translation="MAPGPGHMTYVYDEDVVLAKASHVRRTVETIRSLSSPDNIGVKE
                     WIRDDVTVLNLQRAVEALLDLANHLISTNGWELPRDGRHAFAILRSHGLLTEPEQGLA
                     GAMVGFRNIAVHDYAALDIEIVRGIARDRLGDLESLVASLLGRLRPAATP"
     gene            complement(4303..4683)
                     /locus_tag="PLP50_15330"
     CDS             complement(4303..4683)
                     /locus_tag="PLP50_15330"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPA52963.1"
                     /translation="MAIEDILPALEGASFALVFGSFGTERSRPESDLDLAVRYQKPLT
                     QAQVLDLTHRVSEIAGRNVDLVDLGSADPIIAMQVLRHGRPLLVRDRAAFETFRMTTP
                     SRYFDWKLSRRPVEERLWRQARAT"
     gene            complement(4760..5146)
                     /locus_tag="PLP50_15335"
     CDS             complement(4760..5146)
                     /locus_tag="PLP50_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA52964.1"
                     /translation="MKLLLDTHAFLWWIGDDPRLSDEAREAIADPGNEVTFSVASAWE
                     LAIKSGLGRFEPEGDLGSFLGEQVRQNSFRVLPVTLEHAVEVSSLPNHHRDPFDRLLV
                     AQALVESLILVSRDPQLRKYAAPTLW"
     gene            complement(5143..5379)
                     /locus_tag="PLP50_15340"
     CDS             complement(5143..5379)
                     /locus_tag="PLP50_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPA52965.1"
                     /translation="MRGIVNVHEAKTHLSRLLESVRRGEEVVIGKGGHPIARLVPIEQ
                     PQVRRVPGSARGRFRMSRAFDAPLPKRLVDAFEK"
     gene            complement(5445..6479)
                     /gene="gmd"
                     /locus_tag="PLP50_15345"
     CDS             complement(5445..6479)
                     /gene="gmd"
                     /locus_tag="PLP50_15345"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPA52966.1"
                     /translation="MKKALVTGITGQDGSYLTELLLAKGYEVHGVVRRVSTFNRQRID
                     ALRDGPEGGRLHLHYGDLGDSSSLNRIVRAVVPDEIYNLGAQSHVAISFEVPEYTTDI
                     TAVGAVRLLDAIRDVGLHPRFYQASSSEMFGKVAETPQRETTPFHPRSPYGAAKVYAH
                     SITVNYRESYGLFACSGILFNHESPRRGENFVTRKITRAASRIAHGLEEAVWLGNLDA
                     KRDWGFAGDYVEAMWRMLQHDEPGDYVVATGETHTVREFCEKSFSCAGLPVAWEGEGV
                     NETGRCTKTGKTLVRVDPKYFRPAEVELLLGDPAKAKKVLGWQPTVTFEGLVEMMTRA
                     DLELAAREAA"
     gene            complement(6556..6831)
                     /locus_tag="PLP50_15350"
     CDS             complement(6556..6831)
                     /locus_tag="PLP50_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPA52967.1"
                     /translation="MTWEVVYTLTAKAAIRKLDPGTRNRVRAAVEELSRDPLQGKPLS
                     FTLKGLRSWRTGDWRIVYKAEAERVVVVVVTEGHRRDVYERVRRLLS"
BASE COUNT         1093 a         2110 c         2611 g         1057 t
ORIGIN      
        1 gcctcgatga cctcccagac gtcgatcccc atcttgtcga agacgacctt cagctcgttg
       61 acgagggcga tgttgaccga gcggaaaatg ttctcgagga gcttgccgct ctcggcgacc
      121 tcggccgtcg agacgcggat cactcgctcg agcgctgcgg cgtagagggc ttccgcgacg
      181 tcgcccgagg gggcgtcgat gccgccgacg accttgggga tgttgcgggt gtggaaggac
      241 gggttccccg ggtcttcccg ttcgggcgag aaggcgacga agaccgtctc gccgcacttc
      301 agccccacgg cctcgaggcg ggggaggatc tcctcgcgag tggtgccggg atacgtcgtc
      361 gactcgagga cgacgagctg gccggggcgt gcgacagccg cgatcgcctc ggccgtcgtc
      421 tggatgtagg agatgtcggg ctcgcggtgc ttgccgagcg gcgtggggac gcagatgagg
      481 atggcgtcgc actcggcgag acgggcgaag tcggtggtgg cggcgatgcg gccgcgggtg
      541 aaggcggcgg cgacgcgatc ggggccgatg tgcttgatgt agctctcgcc ccggtggagc
      601 gcctcgggct tcttcgggtc gacgtcgaag ccgaggaccg ggaagcccgc ctcctcgaag
      661 acgagggcga gggggaggcc cacgtagccg aggccgatga tgccgacccg ggcggacctg
      721 gaggcgatct ttccgagcag ctcttcctta gggttcacga gtactcctca cgcggtccgg
      781 atcgccgcag cgcggcgagg gcgaagcgcg gaatgtggtc tgaggggacg gaggacgacc
      841 ctccaaccgt gacgagtctt ccggcccgag aggagacggg cgagccagag ctctccgatg
      901 gaggcggcgt cgatgccccg cgcgacggct cggctgtaga ggagacgttc ggggagatac
      961 aggtcgctcc ggagggactc ggccagatgc ttcattgccg gattcggcca aaggtgggtg
     1021 gtgtagaggg ctgcgaggca gacctggagc gaaactcccc aacggcgtgc ggaggccccg
     1081 gcttcttcga gggccgactc gcctgcgcga cgcgcgatga gctcgaggtc gcgaaagtag
     1141 acgaggttgt gagggggcgg gagctgccag aggtggacgg cggccaggac gaaggcgtcg
     1201 ggccaggagg ggcggcgcgc ctgcgagccc aggtccgggg cgtcgatgca attctcccag
     1261 aggctctcgg cccagctcgg cggggtcgag ggccagaggc ggaagtgcag ctcgaggagg
     1321 atgccggagt cgttccgcgc ctcccagttg taggcctcag atgcgaggaa gcactcgtac
     1381 tcgtcaccgt tgaggagacc tttccacccg gcctcgccga ggaaggcgcg ggcctctgcg
     1441 aaacgtgccg cggggacgag gatgtcgaga tcggaggtgg ggcgcgcctc cggacccggc
     1501 cagaggcggt agccgaggtc catccccttg atgggcatcc aggggatgcc ggcagcgtcg
     1561 aggacgcttc ccacggcatc gagctgctgc ttcaggagca tgcccgcgac ggcggctccg
     1621 atgaggtcat ttcgccacgg cgccgggacc tcgaggccgc tctcgcttgc ggccagagca
     1681 atggccggga tggcccgctt gaatctcggc gccgcgagga gcgccggatc ggcgaccagc
     1741 tcggcaaggg cctcccggtc gcaatccatc gcccgagccg cgagcaggtc gactcttccg
     1801 aggggagagt ccaccgggta cgaatttgag ctgaggccca tggggtctgg tctacgttct
     1861 acagtctgct ccgagacccg gggctcagga gtacacgtgg tcgagtcatc cgatggcctc
     1921 gacgagggcg ccggcgtcga agacgaggaa cgggagccgg gagccgggcc tctccttcgt
     1981 gcgcggcacg aagacggcgc ggacgacttc ttcgctgccg tctccgaccg gcggccgtcc
     2041 tttcgcgagg agctctcgat ggatccgctc gagcgcctct ccggcgacgg gctgatcgtg
     2101 ccatcgcacc tcgccgagga ggagccggcg gccgtcctcg gagagcgcga cgacgtccca
     2161 ctcggagccg gagccgtgcc agtaccggcg ggccggcccc caggccgtgc ctcccctcgc
     2221 gaccgccgag cccaggcgcg gaacggcacg acggcagagg tcttcccagg tctcggagag
     2281 gagccggggg aggtgctcgc gaacgagccg gcgacgagct tcgcgcggcg cgacggcgag
     2341 gagggcgcgg cggggggcga cgatccggaa ccagaaccgg aagaagggat cggcgatccg
     2401 gtagagcgac cgcttgccgc ccttctcggg ctcgccgaag gggagctctc gggagacgag
     2461 gccgagctcg gcgaggcgtg cgagggagcg ggccagcgag gtcgcaggct gcccgatgcg
     2521 gcccgcgatc tcggagaccc ggtgagcgcc ggccccgatg acgtcgagga gcggccgcag
     2581 ggaaacggcc gagggacgtt cctccgcgag gaggcggtcg ggctcgagat ggagcgggcc
     2641 gagggggtcg aggacgagct cgtcgagcgc ctccgggaga tcggctccga agggctcggc
     2701 cagctcccag tagcgtggga tgccgcccca tgccgcgaac gcgcggacgg ctccgagggc
     2761 gtcgccgagg ccgagcgcct ttccgagcgc gcgtggcgtc atcggctcga tcggcatcgc
     2821 cgagacggtc cggccgaaga ggggcgcgcc ggcgccgagg gtgagcccct gcatcatgtg
     2881 ctgggtggag ccggagacgg cgacgaggag gcgagcgtcc ttcgcctcgt gatcgagcca
     2941 gccctggagc acgctgggga gcgaagggct ggtttcgacg aggtagggca gctcgtcgag
     3001 gacgagcggg ccccgccacc ccgctctggt cgcttcgcgc gacagggcgc gcaggaggga
     3061 ccgccagtcg ggccaggcgg cgtctccgaa tccggggaag cggagcgaga gggcgtcggc
     3121 gagataggca cgctgcacgg gggcggagga gaggtcggcc accgagtaga ggccgtcgtg
     3181 gcggcggctc cattcgagga gcagccgggt cttccccact cgccggcggc cccagagcgc
     3241 gacgagcccc tgctggcccc tctcgacgag ggcatcgagg cgcgcgagct cggaggtccg
     3301 gttcacaaag cgcattcgtt attatgcgtc acagaataat gccggccggc atagcggagg
     3361 gatggggtct ggtctacgtt ctacagtcta cggggnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnncggcc tggcgatcga agtcgaatcc gagccggcgg aggcgactgg
     3541 cgacctcccg gtacttgaat ccggagagcc gccccaggac tcagcctccc accacgaggg
     3601 tgagatcgaa gcgatcgacg ggcggcgggt cgggcagagg ctgtctctcg gactgcgctt
     3661 caaggagccg ccgggcgacg tctcgcgcga tctcgagagt ctccgcaatc gttcgaccct
     3721 gagcgaccag accctgcacg tcatcggacg acgcgaggta gacgccctcc ggaagcttct
     3781 cgacgtgtac ggcgatgagg cgttccatgg tccttctccg tccgtgatcg tatcagggag
     3841 gattggggtc tggtctacgt tctacagtct acggggtggc tgccggccga agtcggccca
     3901 gaagcgacgc gacgaggctc tcgagatctc cgaggcggtc ccgggctatg ccgcggacga
     3961 tctcgatgtc gagggctgcg tagtcgtgaa cggcgatgtt ccggaacccg accatcgcac
     4021 ccgccaggcc ctgctcgggc tccgtgagga ggccgtggga acggaggatg gcgaaggcgt
     4081 ggcggccgtc ccttggcagc tcccagccgt tggtggagat caagtggttc gcgaggtcga
     4141 gcaacgcctc gacggcgcgc tggaggttca ggacggtgac gtcgtcgcgg atccattcct
     4201 tgaccccgat gttgtcgggg ctggaaagag atcggatggt ctcgaccgtc cgacggacgt
     4261 gcgaggcctt cgcgaggacg acgtcttcgt cgtagacgta ggtcatgtgg cccgggcctg
     4321 gcgccacagg cgctcttcca cggggcgacg cgacagcttc cagtcgaagt agcgagacgg
     4381 ggtggtcatt cggaaggtct cgaaagcggc ccgatcgcgc acgaggaggg gccggccgtg
     4441 gcgaaggacc tgcatggcga tgatcgggtc tgccgaaccg agatcgacga ggtcgacgtt
     4501 tcgaccggcg atctcggaga cgcggtgagt caggtccagg acctgcgcct gagtcaacgg
     4561 tttctggtaa cggacggcca ggtcgaggtc gctctcggga cgagaacgct cggtgccgaa
     4621 agacccgaag acgagcgcga agctcgcccc ttcgagagcc gggagaatgt cttcgatggc
     4681 cacggggtct ggtctacgtt ctacagtcta cggggccgcg cgcagggacc tgggcatggg
     4741 gttcgtgcag acacagcggc taccagaggg tcggggcagc gtacttccga agctgcggat
     4801 cgcgggagac gagaatcagg ctctcgacca gggcctgagc gacgagaagc cggtcgaaag
     4861 gatcgcgatg gtggttcggc aggctcgaga cctcgacggc gtgctcgagg gtcaccggaa
     4921 ggacccgaaa cgagttctgg cgcacctgct ccccgaggaa cgagccgaga tctccttccg
     4981 gctcgaatcg gccgaggccc gacttgatgg cgagctccca agcgctggcg acgctgaaag
     5041 tgacttcgtt gccgggatcg gcaatggctt cgcgcgcctc atcggacagg cgtgggtcgt
     5101 ccccgatcca ccagagaaac gcatgcgtat cgaggaggag cttcacttct cgaaggcgtc
     5161 gacgagacgc ttcggcaggg gggcatcgaa ggcgcgggac atgcgaaacc gcccccgggc
     5221 gctgcccgga acgcgccgca cctgcggctg ttcgatcggc acgaggcgag cgatggggtg
     5281 ccctcccttc ccgatcacga cctcctctcc tcgacgaacg ctctcgagga gacgggagag
     5341 atgggtcttg gcctcgtgga cgttgacgat gccgcgcatg gcctcattat gacctagtcc
     5401 aggactaagt ccgtttggat ggggtctggt ctacgttcta cagtctacgc ggcctcgcgc
     5461 gccgcgagct cgaggtcggc cctggtcatc atctcgacga ggccctcgaa ggtgacggtc
     5521 ggctgccagc cgaggacctt cttcgccttg gccgggtcgc cgaggaggag ctcgacttcg
     5581 gcggggcgga agtacttcgg gtcgacgcgg acgagggtct tgccggtctt cgtgcagcgg
     5641 cccgtctcgt tcacgccctc cccttcccac gcgacgggga ggccggcgca ggagaacgac
     5701 ttctcgcaga actcgcggac ggtgtgggtc tcgccggtgg cgacgacgta gtcgcccggc
     5761 tcgtcgtgct ggagcatgcg ccacatggcc tcgacgtagt cgcccgcgaa gccccagtcg
     5821 cgcttcgcgt cgaggttgcc gagccagacg gcctcttcga gaccgtgggc gattcgcgag
     5881 gcggcgcggg tgatcttgcg ggtgacgaag ttctcgccgc ggcgggggct ctcgtggttg
     5941 aagaggatgc cgctgcacgc gaagaggccg tagctctcgc ggtaattgac ggtgatggag
     6001 tgcgcgtaga ccttggccgc tccgtagggg ctgcgcgggt ggaagggagt ggtctcgcgc
     6061 tggggggtct cggcgacctt cccgaacatc tcggaggagg aggcctgata gaagcgcggg
     6121 tggaggccga cgtcgcggat ggcgtcgagg aggcgaacgg cgccgacggc ggtgatgtcg
     6181 gtggtgtact cggggacctc gaagctgatg gcgacgtggc tctgggcgcc gaggttgtag
     6241 atctcgtcgg ggacgacggc gcggacgatg cggttgaggg aggaggagtc gccgaggtcg
     6301 ccgtagtgga ggtggaggcg gccgccttcg gggccgtcgc ggagcgcgtc gatgcgctgg
     6361 cggttgaagg tggagacacg gcggacgacg ccgtggacct cgtagccttt ggcgaggagg
     6421 agctcggtga ggtacgagcc gtcctggccg gtgatgccgg tgacgagggc tttcttcaag
     6481 cgtgtggcct ttcccttgtt tggtctcgag ggcggtctcc ccttcccgcg gggccgggcc
     6541 gccgtggcat tcgtgtcagg agaggagccg gcggacccgc tcgtagacgt cgcgccgatg
     6601 gccctcggtc acgacgacga cgacgaccct ctcggcctcg gctttgtaga cgattcgcca
     6661 gtctccggtg cgccaggagc ggagcccctt cagcgtgaaa gagaggggct tgccctgaag
     6721 agggtcccgc gagagctcct cgacggcggc gcgcaccctg ttgcgtgttc ccggatcgag
     6781 cttcctgatg gccgccttcg ccgtgagcgt gtagacgacc tcccaggtca cccccagacc
     6841 tcttcgtggc cgacgatgcg acccttctcg acgtcgtcca ggccgcgccg gacgctcttc
     6901 accagctcgg cgtccgccag gatctccagg gtctcgagga gcccctcgta ctcgtcgacg
     6961 gacatgagga c
//
LOCUS       DAOONL010000157         6820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13233_length_6820_cov_17.901700, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000157 DAOONL010000000
VERSION     DAOONL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13233_length_6820_cov_17.901700"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2401)
                     /locus_tag="PLP50_15355"
     CDS             complement(<1..2401)
                     /locus_tag="PLP50_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52968.1"
                     /translation="MRRRALLLGLPLLLTSSLLAQTHPNLERGLAVGKAYQVGDVDSV
                     NLFNGNLSLTIPLGQSYHAGGDLSYSFVLRYNSNVWDHNLVTFTNDCTPPYKSITVTQ
                     TTPHALTNGLNAGLGWSISFGELMNTGWGTGSDRWTYTSPDGSTHPFYNTLHDGETER
                     IGYRYTRDGSYMRLIASDEGSSAGCPSGWRAATVEFADGRRQTFSRTGTCPGHPFTLN
                     SEMDRYGNSLTFSYDTANNRWVIQDSTGRTHYIGYRGLSGLDYGGIDYVDLAAFGGTR
                     ARYDFHYSNAAVARSCKHYFDPWWNSCDPQLPVDIPAMAFLYEVILPDGSKWTTKEGT
                     DLGYNLNVHDDCLAGGIPQDQPGTLKYLKNPVGGTYSWTYATWTNPSGQVCSDPTDAY
                     TKEATAVAQRTLTEPLGSGGSGTWTYTHDSWRLGEGTVDPSSQESWTVVRTPEGDESK
                     HWFRTQYCGTFPEGWDYGLPYTKGRYGTAAEPWISTQQYDGLSSTPSNLKRTTYLRYD
                     KDALNSSWSPSFLQQSNRRVDYERTVYADDGSRYTTATYTGFDGLGHYRTVTTGGNFG
                     SGNVRQTTTTYNGTLSYPPASPPAEWWWYASPWVINTYQSSTVTEGGSVAKSEACFDS
                     LTGYLLRTRTLKLIGVGADPAQSTSDVITRRTHTAGNTTREESFGGDTQTVGTGALCS
                     LTLPTAAYRVDHTYQYGSLKTSRYVNSSGTPLSHFNVDVDLDANTGLVATSRDSAGLA
                     TDYLYDNMGRLTWEKPRTTPVNGGSWVQHCYINYVDGPTKVPARVDNYWWPNGVTQPC
                     LT"
     gene            complement(2406..3089)
                     /locus_tag="PLP50_15360"
     CDS             complement(2406..3089)
                     /locus_tag="PLP50_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52969.1"
                     /translation="MIPRIALGSAIAFLLATTSGPLSAQSGPAPGKLSIGFDSEGAVV
                     EGITPGGNVAWLGASRSHEEWGEHLAHWRLLTGDDDKDRIVRFPREKGFPEMTILAAI
                     DLTSGTWAVGATYPLEGEVPAPDLGHARRDSSGLVDTISVRAPRVDLLVVRPGADAWS
                     ARILDGSPLDLDAATDGVVTVGLSVLPSRTAKATRLDGIRPGDIVAMTDARGPWVHVA
                     RITAAGGKE"
     gene            3333..3851
                     /locus_tag="PLP50_15365"
     CDS             3333..3851
                     /locus_tag="PLP50_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA52970.1"
                     /translation="MDESDERLLSGHEAAAELASDPEQRRKLVSYAWQKFGLSSDEAG
                     EVIQETCIDLMHLRQLVRSREGLVFRIFSLRCSKRLGSRKRRPNSAEPERVLARQESR
                     DLPADDRVLLREAFEEMSPTCRRLVLALYIEGISPSEMARAGTWASPKVVSNLTSRCI
                     RKLRERFRGARG"
     assembly_gap    3950..4049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4050..5181
                     /locus_tag="PLP50_15370"
     CDS             <4050..5181
                     /locus_tag="PLP50_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52971.1"
                     /translation="AAEERDTIPGHLRAFLLARPEERLALEYALDEALERMVERPARW
                     LSFGRAAAARIRAEIPAGGDDAVEAESLCPLPEVDGVASLVVGSACNWTGAFSEGGRA
                     LAHAYRAFAAGLGLSPRFAQVELAESQRRTFLGRPAEGLRLANRAAEAFQEYGLEAHA
                     ARARGARALAFSYLGRDEEAVSEFRGAREAFERQGQWNAWVSALNGAGSSLMRLGRIE
                     EARREYARALRRVSRTERPAVHAFIRSNLAKTLLAAGRVEDAARGFSSAASLFLDQGA
                     VVDALTALLGEVDARARAGQAAAARRAFGRFRAMLEREGAADALLVTSLETALAADRF
                     DLALLATLREQAEVSLRDGGALIRTRPSRDRPEYQNRPSGGA"
     gene            5184..5705
                     /locus_tag="PLP50_15375"
     CDS             5184..5705
                     /locus_tag="PLP50_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPA52972.1"
                     /translation="MDAKARTPTTRRELAETIAAEGDLRRRLAAHARLRFRLPASAAE
                     DLVQDVCLELMRRDGPVRDPEALAFRILRYECLHLLRSARWRREVSDEEVSGLADRTS
                     APGASIELLALVRQAVAGVSPACRRLLHSHYVEGRTLKETARALSYASPNNVWALLAR
                     CVQRLRAALGANR"
     gene            5702..>6820
                     /locus_tag="PLP50_15380"
     CDS             5702..>6820
                     /locus_tag="PLP50_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA52973.1"
                     /translation="MSAAHPDDLTLLMLLSGETSELETARLRRHLGECPACSSALSDL
                     HELDRTLKATLPELEAALSERSLPEGDPFRCRPDEARPRRRPGRPTDEVVTEALAASR
                     SADGVKERLLGAVRSPDDALREALAALDLLEVRDRFGLGYALDEALYSMVDGPARWAR
                     FGRASRDTVLAARSDPRIRDASAEVAYPLDDLLGLSLLVEGTACNWTGDLVRGGRALR
                     RSYGAFARGGSSDYRFASVELSESQRRAFLDRAEEALHLAERARLTFEALGLDDERAR
                     AAGAHALALSYLGRDDEALVEFRSTLPALAAAERWNAYASVLNGIGGCLLRIGRPQEA
                     RREYARALRQVSRQARPAVHAFIRANLARALFQAGRYEE"
BASE COUNT          914 a         2269 c         2416 g         1121 t
ORIGIN      
        1 aggtgaggca gggttgcgtg accccgttcg gccaccagta gttgtcgacg cgggcgggga
       61 ccttcgtggg cccgtcgacg tagttgatgt agcagtgctg gacccaggag ccgccgttga
      121 ccggcgtcgt ccggggcttc tcccaggtca gccgtcccat gttgtcgtaa aggtagtccg
      181 tggcgaggcc ggcgctgtcg cgactcgtgg ccacgaggcc ggtgttcgcg tcgaggtcga
      241 cgtcgacgtt gaagtgcgaa agcggcgtcc cggaggagtt gacgtaccgg gaggtcttca
      301 ggctcccgta ctggtaggtg tggtcgaccc gataggccgc ggtcggcagc gtcagggagc
      361 agagggcgcc ggtgccgacg gtctgcgtgt cgccgccgaa ggactcctcc cgagtggtat
      421 tgccggcggt gtgggtccgg cgtgtgatca cgtcgctcgt cgactgcgcg gggtcggccc
      481 cgacgccgat cagcttcagc gtccgggtcc gaagcaggta gccggtgagc gagtcgaagc
      541 acgcctcgct cttggcgacg cttccgccct ctgtgaccgt cgacgactgg taggtgttga
      601 tgacccaggg gctggcgtac caccaccact ccgcgggggg agaggccggc ggatagctga
      661 gcgttccgtt gtaggtcgtc gttgtttggc ggacgttccc gctgccgaag ttcccgccgg
      721 tcgtgacggt ccggtagtgg ccgaggccgt cgaatccggt gtaggtggcc gtcgtatagc
      781 ggctcccgtc gtcggcgtag acggtccgct cgtagtcgac ccgacggttc gactgctgga
      841 ggaagctggg ggaccagctc gagttcagag cgtccttgtc gtagcgaagg taggtcgtcc
      901 gcttcaggtt cgagggggtg gaggacaagc catcgtactg ctgcgtggag atccacggct
      961 cggcggcggt gccgtagcga cccttcgtgt acggcagccc gtagtcccag ccctccggga
     1021 acgtgccgca gtactgcgtc cggaaccagt gcttgctctc gtcgccctcc ggcgtccgga
     1081 cgacggtcca cgactcctgc gacgaagggt cgaccgtgcc ttcgccgagc cgccaggaat
     1141 catgcgtgta ggtccaggtg ccgctcccgc cgctgccgag cggttcggtc agggtccgct
     1201 gcgcgacggc cgtcgcttcc ttggtgtagg cgtcggtcgg atctgagcag acctggccgg
     1261 aggggttcgt ccaggtggcg taggtccagc tgtacgtccc cccgacggga ttcttgaggt
     1321 acttgagggt accgggctgg tcctgcggga tgccgcccgc gaggcagtca tcgtggacgt
     1381 tcaggttgta cccgaggtct gtgccctctt ttgtagtcca cttcgagccg tcagggagaa
     1441 tgacttcgta caggaacgcc atggccggga tgtccacggg aagctgcgga tcgcaggagt
     1501 tccaccacgg gtcgaagtag tgcttgcagc tccgggcgac ggctgcgttg ctgtagtgga
     1561 agtcgtaccg ggcgcgcgtg ccgccgaagg ccgcgaggtc gacgtagtcg atcccgccgt
     1621 agtcgagccc gctcagtcct ctgtagccga tgtagtgggt ccggccggtg ctgtcctgga
     1681 tgacccagcg gttgttcgct gtgtcgtagg aaaaggtgag cgagtttccg tagcggtcca
     1741 tctcggagtt gagggtgaag gggtgcccgg ggcacgtccc cgtccgggag aaggtctgcc
     1801 ggcggccgtc ggcgaactcc accgtggccg cgcgccagcc cgacgggcag ccggccgagg
     1861 agccttcgtc cgaggcgatg aggcgcatgt agctgccgtc gcgggtgtag cgatagccga
     1921 tccgctccgt ctccccgtcg tgcagcgtgt tgtagaaggg gtgggtgctt ccgtcgggtg
     1981 aggtgtaggt ccatcggtcc gagccggtgc cccagccggt gttcatcagc tcgccgaagc
     2041 tgatgctcca cccgaggccg gcgttcaggc cgttcgtcag ggcgtgaggc gtcgtctggg
     2101 tgacggtgat cgacttgtag ggcggggtgc agtcgttcgt gaaggtgacg aggttgtggt
     2161 cccagacgtt cgagttgtag cggaggacga aggagtagga gaggtcgccc cccgcgtggt
     2221 agctctggcc gagggggatc gtgagggaga ggttgccgtt gaagaggttg acgctgtcga
     2281 cgtcaccgac ctggtacgcc ttgccgacgg cgaggccgcg ctcgaggttc gggtgggtct
     2341 gggcgaggag tgagctcgtg aggaggagcg ggaggccgag gagaagcgcg cgtcgcctca
     2401 tgacctcact ccttgccccc ggcggcggtg atccgggcga catggaccca ggggccgcgg
     2461 gcatccgtca tcgccacgat gtcgccgggg cgtatcccgt cgaggcgtgt ggccttcgcg
     2521 gttcgcgagg gcagcaccga gaggccgacg gtgacgacgc cgtccgtcgc cgcgtccagg
     2581 tcgagggggc tgccgtccag gatgcgggcg ctccaggcgt cggcgccggg tcggaccacg
     2641 aggaggtcga cgcgaggggc ccgaacggag atcgtgtcga cgagaccgga ggagtcgcgc
     2701 ctcgcgtggc cgaggtccgg tgcggggacc tcgccttcga gcggataggt cgcgcccacg
     2761 gcccaggtgc cggaggtcag gtcgatcgcc gcgaggatcg tcatctccgg gaaccccttc
     2821 tcgcgcggga agcggacgat gcggtccttg tcgtcgtcgc cggtcagtag ccgccagtgg
     2881 gcgagatgct cgccccactc ttcgtgactc cggctcgcgc cgagccaggc gacgttgccg
     2941 ccgggggtga ttccttcgac gacggcgcct tcggagtcga agccgatgct gagcttcccg
     3001 ggagcgggac ccgactgggc cgagagaggg cctgaagtcg ttgcgagcag aaaggctatg
     3061 gcacttccca gggcgattct cggaatcaca ggacctcctt gctgccagtc gatcgcagca
     3121 acacacgcga tttcgggtcg gcccgcttcc cgctgagatg aagtcctcgc tcgatggacg
     3181 atgacgggcc ttccctcctc cacctgaagt gcccgcagcg cccggatctc caacacccgg
     3241 ggggcgaaat tcacgacgtg ccgccccctg ccccaccgga gattcgcgtc tatcctcccg
     3301 ttggatctcg gacctgcgga ggcacttcag acgtggatga gtcggacgaa cggctgctct
     3361 ccggtcacga ggcggccgcc gagctggcct cggaccccga gcagcgcagg aagctcgtct
     3421 cctacgcctg gcagaagttc gggctctcga gcgatgaggc aggagaggtc atccaggaga
     3481 cctgcatcga cctgatgcat cttcgccagc tcgtccgaag ccgcgaaggg ctcgtcttcc
     3541 ggatcttctc cctccgctgc agcaagcgcc tcgggtcccg gaagcgtcgc ccgaattcgg
     3601 ctgaacccga gcgcgtcctc gcccgtcagg agagtcgtga tctccctgcc gacgatcgag
     3661 tcctgctccg ggaggcgttc gaggagatgt cgccgacctg ccggagactc gtgctggctc
     3721 tctatatcga agggatctcg ccgtcggaga tggcccgagc aggcacatgg gcttcgccga
     3781 aggtcgtctc gaacctgacg agccgctgca tccggaagct gcgcgagcga ttccgaggag
     3841 ctcggggatg acacctcacc ccgacgacct ctccctcttg ctccaggtgc ggggtgcgct
     3901 cgacgaggtg acgcgcgccc gcttgctggc gcatgtcgca ggctgcaccn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccgccgagg agagggacac gatcccgggt
     4081 caccttcggg cgttcctcct ggctcgaccc gaggagcgcc tggcgctcga gtacgccctc
     4141 gacgaggcat tggagcggat ggtggagcgt ccggcccgct ggctgtcgtt cggccgagcc
     4201 gccgccgcgc gcatccgcgc cgagatcccg gcgggcggag acgacgcggt ggaggccgag
     4261 agcctctgcc cgcttcccga ggtggacggc gtcgccagcc tcgtcgtcgg ctcggcctgc
     4321 aactggacgg gcgctttctc cgaaggtggt cgggccctgg cgcatgccta ccgggcgttc
     4381 gcggccggcc ttggcctctc tccccgcttc gctcaggtcg agctggccga gtcccagcgc
     4441 aggaccttcc tcggtcggcc tgccgaaggg ctccgtctcg ccaaccgagc cgcggaggcc
     4501 ttccaggagt acgggctcga agcccacgct gcgcgcgccc gaggagccag ggccctggcg
     4561 ttctcctacc tgggacgcga cgaggaggcg gtctcggagt tccggggggc ccgcgaggcg
     4621 ttcgagaggc aggggcagtg gaacgcctgg gtgtccgcgc tgaacggcgc gggctcttcg
     4681 ttgatgaggc tcgggcggat cgaggaggct cgccgcgagt atgcccgggc actgcggcgc
     4741 gtctcccgca ccgaacgccc cgccgtccac gcgttcatcc gctcgaacct ggcgaagacc
     4801 cttctcgccg ccggccgcgt cgaggatgcg gcacgcggct tttcgtctgc cgcgtcgctc
     4861 ttcctggatc agggcgccgt cgtcgacgct ctgacggcgc tgctcggcga agtcgacgcg
     4921 cgggcgcgag ccggccaggc tgcggctgcg aggcgggcat tcggtcgatt ccgggcgatg
     4981 ctggagcggg agggagcggc agacgccctg ctcgtcacct cgctcgagac tgcgctcgcg
     5041 gccgacagat tcgacctcgc gcttctcgcg actttgcgcg agcaggccga ggtctcgctg
     5101 cgggacgggg gcgccctgat tcggacacgt ccttcgcggg atcggcccga gtatcaaaat
     5161 cgtccctctg gcggcgcctg atcatggatg cgaaggcccg gaccccgacg acccggcgag
     5221 agctggccga aacgatcgcc gcagagggag acctccgccg gcggctcgcg gcccacgccc
     5281 gcctccggtt ccggctcccg gcctccgctg cggaggacct cgtgcaggac gtctgcctcg
     5341 agctgatgcg acgcgacggc ccggtccgcg accccgaggc tctcgcgttc cggatcctcc
     5401 ggtacgagtg tctccacctc ctccggagcg cccgatggcg gcgcgaggtg agcgatgagg
     5461 aggtctccgg cctcgccgac cgcaccagcg cccccggcgc ctcgatcgag ctgctcgctc
     5521 tcgtccgcca ggccgtcgcc ggcgtcagcc cggcctgccg ccggctcctc cactctcact
     5581 acgtcgaggg ccggacgctg aaggaaacgg ccagagccct ctcctacgcc tcgccgaaca
     5641 acgtctgggc cctcctcgcc cgctgcgtgc agcggctgcg agcggccctg ggagcgaatc
     5701 gatgagcgct gcccatccgg acgatctcac gctcctgatg ctcctctccg gagagacctc
     5761 cgagctggag accgctcgcc tgaggcggca cctcggcgaa tgcccggcct gctcctcggc
     5821 cctttccgac cttcacgagc tcgaccggac gctgaaggcg actcttcccg agctggaggc
     5881 cgcgctctcg gagcgctccc ttccggaggg agatccgttc cgttgccggc cggacgaggc
     5941 ccggccgagg cgaaggcccg gacgtccgac cgacgaggtc gtcaccgaag ccctcgccgc
     6001 gagccggagc gccgacggtg tgaaggagcg tctcctgggc gccgtccgct ccccggacga
     6061 cgctcttcgc gaggcgctcg ccgccctcga cctcctcgag gtccgtgacc ggttcgggct
     6121 cggctacgcg ctggacgagg ctctctattc gatggtggac gggcccgccc gctgggcccg
     6181 cttcggccgc gcatcccgcg acaccgtcct cgcggcgcgc tcggatcccc gtattcgcga
     6241 cgcctccgcc gaggtcgcct acccgctcga cgacctcctg ggcctgtctc tcctcgtcga
     6301 agggaccgcc tgcaactgga cgggcgacct cgtccgagga ggccgcgcgc tgcgccgctc
     6361 ctacggcgcc ttcgcacggg gcggctcttc ggactaccgc ttcgcctccg tcgagctgtc
     6421 cgagtcgcag cgccgggcgt tcctggatcg ggccgaagag gcgctccacc tggccgagag
     6481 ggcgaggctg acgttcgagg cgctcggcct ggacgacgag cgcgcgcggg ccgcgggggc
     6541 ccacgccctg gccctgtcgt atctcgggcg cgacgacgag gcgctcgtcg agttccgctc
     6601 cacgctcccc gcgctcgccg ccgccgagag gtggaacgcc tacgcctccg tcctgaacgg
     6661 catcggcggc tgcctgctcc ggatcgggag gccgcaggag gcccggcggg agtacgcccg
     6721 cgccctccgc caggtcagcc gtcaggctcg ccccgcggtc cacgccttca tccgcgccaa
     6781 tctcgctcga gcgctcttcc aggcgggacg ttacgaagag
//
LOCUS       DAOONL010000158         6812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13257_length_6812_cov_8.276306, whole genome shotgun sequence.
ACCESSION   DAOONL010000158 DAOONL010000000
VERSION     DAOONL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13257_length_6812_cov_8.276306"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLP50_15385"
     CDS             complement(<1..243)
                     /locus_tag="PLP50_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52974.1"
                     /translation="MTDVARQVEALPPATDPEPETRLAELAHELALSREIRRRLEAES
                     RRWEARAAALDAEVHHLRGVLAERERHISAVERSVVW"
     gene            303..1157
                     /locus_tag="PLP50_15390"
     CDS             303..1157
                     /locus_tag="PLP50_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA52975.1"
                     /translation="MTSAPRPTDVRPADGPVYDSARVEIPIVSEAMALWEYRHLVREL
                     VARDVKVRYKRSVLGVAWTMLNPLLNMAALTVVFSVILRQDIVHFPVYFLGGMLFWNF
                     FAQSTGHAASLSVDAAETTRRIYVPRSVFVVAAVGVGLVNLAFSLVPLALIVAVTRHP
                     FHVTWLFLPVAVLLGAMFSTGVGLVVFTLASRFVDVRETYVTLLTPWLFLTPIVYTPS
                     LVPEGYRWIVRWNPMTYLVEVFRAPIYDGWLPGWKTLSFAVVVAVVSLGLGWAFYASR
                     IEEYGRGA"
     gene            1147..1959
                     /locus_tag="PLP50_15395"
     CDS             1147..1959
                     /locus_tag="PLP50_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA52976.1"
                     /translation="MAPDAQTELPAIQLEGVSVRYRVPKEVVSSIKEYAIRKLRGRIE
                     HDELVALRGVSVSVAPGDRIGVVGPNGAGKSTMMKVIARVCRPSEGRVVVRGRVAPLL
                     QLGIGFHSELSGRENVVLQGTLLGRSRAEMETRLGEIAAFAEVEAFLDAPVRTYSTGM
                     VARLAFATATDVDPDILLIDETLSVGDERFVTKCYERMAGFRDRGKTFFLVSHSLAEV
                     EKSCHRAIWLLDGEVVADGEPMEVCERYREWARNGAVAPLPNPGTAAVSLAG"
     gene            2916..3935
                     /locus_tag="PLP50_15400"
     CDS             2916..3935
                     /locus_tag="PLP50_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52977.1"
                     /translation="MSFTGMGRTARTCPLTHRPTRGLAGSRAGLAVVAFLALGIPDAR
                     ADELGDALKGRVQEVLDGKRSLAEVRIEVVAGRPNRRGLTIYGSGVGTWNQEKQFVLS
                     PDTHRELLKKLLASGLFDMPEKPKPPRQVEMNPPVVVRSVGVRVGELERTVSQNTRVW
                     PLAALEQLVADLFELCRPAVERGTSAVTLTDGLEKVAAGKLAPEAFSLVLNIPPAASG
                     PNADGLLAEIEAGVLTWRVQPPGKAPPVVRRALTSEQIRGIAARIAAADFERFPPNLH
                     RERYVDLRTAVLNRVHSVQARRFARPTPADSTDRQKALDELVAAVRALEPSEAAGEEP
                     KGEKR"
     gene            4273..>6812
                     /locus_tag="PLP50_15405"
     CDS             4273..>6812
                     /locus_tag="PLP50_15405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPA52978.1"
                     /translation="MKRVLSAIAVAGLVMTGTLSAEMLPETKGRAQVLRPVKMDVSPA
                     LRDIPVPPLKPFDAREIPNKTTPQRPATPGTSEKIQTTPGLPNTPDPLAGWVGLGSDD
                     NQAIIGGRLMPPDTQGEIGKDHYIQWINLVFAIWDKAGNKVYPPQPGPAGAAGNVLWS
                     GFGGRCETDNSGDPITLWDPLAERWVMSQFAVDSTPFTQCVAVSTTSDPTGTWYRYAY
                     AWPNSRFPDYPKLGVWPDGYYITTNDFNSSLTSFVGVTVGAFERDKMLVGDPAGFIFF
                     QTSGDREYSVLPADLDGMTPPPAGAPGLIGEFQDSTWIGTTNDQIWLFELNLDWTNPA
                     NSTFGVGATHDPNWAIDISNVSANCSGRDCVPQGPSSQKVDDIDFRLMHRLAYTNLGS
                     SQALVASMTVGAGGRAGVWWGELQSPNPGPTWTLRQDGVYAPADGDHRWMPAIAMDIN
                     GNIMLGYSASSTTVYPSVRYVGRLAGDPLGQMGDEKVLVVGSGAQSGGNRWGDYSMMS
                     VDPTDGCTFWYTQEYIAASGNYDWDTYIGSMKFPSCTAGPAGVIEGYVRSTANNSPIA
                     GASVQIGGSYSTSTNASGFYQLTVPVGTYDVTASKFGYGPASIAAQEVTEGATTVVAD
                     LLLSPVGSYAIDGFVTAEAHNWPLWAKVEVRQNGTLVETVYTSPWNGYYEIPALPNGF
                     TYDFTVTSMYVGYQPETRVVTIASGDQIQSFSLLAASGNPAYNCVLQGGINEDFNADF
                     PPLGWTVKNNTSNSGNTWKRNDVWGRANLTGGTGTAAAADSDKAGSGSGTFDTELWSP
                     VIQMPAEPRNLVFRSAYTQYTGQTGTLDVSTNGGNTWTNLLTITTSATG"
BASE COUNT         1037 a         2269 c         2436 g         1070 t
ORIGIN      
        1 ccagacgacg gagcgctcga cggcggagat gtgccgctcg cgttcggcga ggacgccccg
       61 gaggtgatgg acctcggcgt cgagggccgc ggcgcgggcc tcccaacgcc gggactcggc
      121 ctcgaggcgt cggcggatct cgcgactcag cgcgagctcg tgcgcgagct cggcgaggcg
      181 cgtctccggt tccgggtcgg ttgcgggggg gagggcttcg acctgccggg caacgtcggt
      241 cacgacgtga gaatatcgtc ccacgacccc cccgacgacg ggcgaggctc ctgacgaacc
      301 gaatgacgag cgccccccga cccaccgacg tccgccccgc ggacggcccc gtgtacgact
      361 cggcccgggt cgagatcccg atcgtctcgg aggcgatggc gctctgggag taccggcatc
      421 tcgtacggga gctcgtcgcc cgcgacgtga aggtgcgcta caagcggtcg gtcctcggcg
      481 tggcgtggac gatgctgaac ccgctcctga acatggcggc cctcacggtg gtcttctcgg
      541 tgatcctccg gcaggacatc gtgcacttcc cggtctactt cctggggggg atgctcttct
      601 ggaacttctt cgcgcagtcg acgggccacg cggcgtccct ctccgtcgac gccgcggaga
      661 cgacgcggcg catctacgtc ccgcgctcgg tcttcgtggt ggccgcggtc ggggtcggcc
      721 tggtgaacct cgccttctcg ctcgtcccgc tcgccctcat cgtcgcggtg acgcgccacc
      781 cgttccacgt cacctggttg ttcctccccg tggcggtgct tctcggcgcg atgttcagca
      841 cgggggtggg cctcgtggtc ttcacgctcg cctcgcggtt cgtcgacgtg cgcgagacgt
      901 acgtcacgct cctgacgccg tggctcttcc tgacgccgat cgtctacacg ccctcgctcg
      961 tgccggaggg ttaccgctgg atcgtccgct ggaacccgat gacgtacctc gtcgaggtct
     1021 tccgcgcgcc gatctacgac ggctggttgc cgggatggaa gacgctctcc ttcgccgtcg
     1081 tcgtggcggt cgtcagcctg ggcctcggct gggcgttcta cgcgtcgcgc atcgaggagt
     1141 acgggcgtgg cgcctgacgc ccagaccgag ctcccggcca tccagctcga aggggtgagc
     1201 gtccggtacc gcgtgccgaa ggaggtcgtg tcgtcgatca aggagtacgc gatccggaag
     1261 ctccgggggc gaatcgagca cgacgagctc gtcgcgctcc ggggcgtctc ggtgagcgtc
     1321 gctccgggcg accggatcgg ggtcgtcggg ccgaacggag cgggtaagag cacgatgatg
     1381 aaggtcatcg cgcgcgtctg ccgtccctcg gaagggcgtg tcgtcgtccg tgggcgcgtc
     1441 gcgccgctcc tgcagctcgg catcgggttc cacagcgagc tttccggtcg tgagaacgtg
     1501 gtgctccagg ggacgctcct cggccggtcg cgggcggaga tggaaacccg gttgggggag
     1561 atcgccgcct tcgcagaggt cgaggcgttc ctcgacgccc cggtgcggac gtactcgacc
     1621 gggatggtcg cgcgcctcgc gttcgcgacc gcgacggacg tcgacccgga catcctgctc
     1681 atcgacgaga ctctctccgt gggcgacgag cgtttcgtga cgaagtgcta cgagcggatg
     1741 gccgggttcc gggacagggg gaagacgttc ttcctcgtgt cgcactctct cgccgaggtg
     1801 gagaagagct gccaccgcgc gatctggctc ctcgacggcg aggtcgtcgc cgatggcgag
     1861 ccgatggagg tctgcgagcg ttacagggag tgggcccgga acggggctgt cgcgccgctt
     1921 ccgaaccccg gaacggccgc ggtctccctg gccggctgag cccccgttcc ccccggcccg
     1981 aggggcgtca cccccggaat cgccttcttg acgaggcctc gaagccgggc atggattgcc
     2041 tccgagcctc accacggccg gctcgttgag cccgacgacg gatgaccgct ccggagctct
     2101 tcccgcttgc tcccccccgc cacggatggc ttcggaacgc agaagacagc ccagcctcgc
     2161 cggcgaagcg gacgtccgtt ccggtcggcg cgacgctcac cttctccgag cggaggcgca
     2221 acccgaatag gcggcaatag gcacggcctc tgggcctgcg cggccgacgg cggcagcctc
     2281 acggagatcg ccgagcgcgg gccgcctggt ctgacgctcc cccgcggcac ggacgtcacc
     2341 cgtgccagcg tccgcgtgtc gagcgcgtcc gcaggcggag gagaggactg tagccacgag
     2401 ctcctctgat cggcgcccgg agaagacgat tcctcgcgcg ctggcttccg gagtcggctc
     2461 cggcggctgg cgaagggcgt ggggagccga acggaggcgt gggccgcgcg gaatcgggac
     2521 cggacgaggt cgagaaggca atccgggaag agcaacttct tgacctgacg atggccgggg
     2581 cctatggtct cccctatgcc tcaatacgtc agcagctccg gtcccatcaa gagcggtcgg
     2641 tagcgacggc gcccatgtgc cgtggcgctc ggggaggcaa gggcgtcgcg gccgtcgtcg
     2701 cttggagtga gagtgatccg tccttccgct gaggagcggg ttcttcgttg tcccgcagcg
     2761 tcgacctcga gtcggcgact cgtcggctct cgccctgtcg agggcgcgca cgctcgggtc
     2821 ggggtctccg agagtgcttc gtaccgccac gttgtcctcg cgagcgacgc gatcccgcgg
     2881 accgagtcgg tgtggcctgg gaggggggat tcgagatgag cttcactgga atggggcgga
     2941 ccgcgaggac gtgcccgctg acgcatcgtc cgactcgcgg cctggccggg tcgcgggccg
     3001 ggctcgcggt cgtcgccttc ctcgcgctcg gaatcccgga tgcgcgcgcg gacgagctcg
     3061 gcgacgcgct gaagggacga gtccaggagg tcctcgatgg gaagcggtcc ctcgcggaag
     3121 tccggatcga ggtcgtcgca gggcgaccga atcgccgggg gctcacgata tacggatcgg
     3181 gagtcgggac ctggaaccag gagaagcagt tcgtcctctc tccggatacg caccgggagc
     3241 tcttgaaaaa gctcctcgcg tccggcctct tcgacatgcc ggagaagccg aagccgcctc
     3301 gccaggtcga gatgaacccg cccgtcgtgg tccgctccgt cggcgtccgc gtcggcgagc
     3361 tggagaggac cgtctcgcag aacacccgtg tgtggccgct cgcggcgctc gagcagctcg
     3421 tcgcggatct cttcgagctc tgccggcccg cggtggagcg ggggacctcc gccgtgaccc
     3481 tcacggacgg gctcgagaag gtcgcggcgg ggaagctcgc gccggaagcg ttctcgctcg
     3541 tcctgaacat tccgccagcg gcgtccggac cgaacgcgga cggtctcctc gccgagatcg
     3601 aggcgggcgt cctcacgtgg agggttcaac cgccggggaa ggccccgccc gtggtccgtc
     3661 gcgcgctcac gtccgagcag attcgtggga ttgcggcgcg gatcgcagcc gctgacttcg
     3721 agcgcttccc gcccaacctg caccgggaac gctacgtcga cctgcgcacg gccgtcctga
     3781 accgggttca ctccgtgcag gcccgccggt ttgcgcgccc cacgccggcg gattcgaccg
     3841 atcggcagaa ggccctcgac gagctggtcg ccgccgtccg ggcactcgag ccgtctgagg
     3901 cggcaggcga ggaaccgaaa ggggagaagc gctgatcgtt tcgtgcgaca gccgctgccg
     3961 aggccggctc ggatcgcccg ggcttcaaga ggagcccggc cttcggtctc acgggacgaa
     4021 ttcgcggctc gccggcggaa gcgtcggcac gacggaaacg gtcggacgat gggcgccatt
     4081 ccgcctgcct ctcctgccgg cctccccgag cttcaggtcc agtcactccg gccgcgaggc
     4141 ggccgatctt tcgatgtgga tcccgtcacg gggtcgtgaa aggaggtcct tcccgcgtaa
     4201 cccgatttcg gattctctcg atgtccactt cctggtgacc aacatgaaat gggaggaaag
     4261 gtgagattca gtatgaagcg tgtgctgtcc gcgatagcgg tcgccggtct ggtgatgacc
     4321 gggacgctgt ctgcagaaat gcttccggag acgaagggcc gagcgcaggt gctgcggccc
     4381 gtgaagatgg acgtgtcacc ggctctccgg gacatcccgg tcccgccgct gaagccgttc
     4441 gatgctcgtg agatcccgaa caagacgacg ccgcagcggc cggccacgcc ggggacgtcg
     4501 gagaagatcc agacgacccc cgggctgccg aacacgcccg acccgctcgc gggctgggtg
     4561 ggactgggca gcgacgacaa ccaggcgatc atcggcggcc ggctgatgcc gcccgacacc
     4621 cagggcgaga tcggcaagga ccactacatc cagtggatca acctggtctt cgcgatctgg
     4681 gacaaggcgg gcaacaaggt ctatccgccg cagccgggcc cggccggagc ggccggcaac
     4741 gtcctgtgga gcgggttcgg cggtcgctgc gagacggaca actcgggcga cccgatcacg
     4801 ctgtgggacc cgctggcgga gcggtgggtg atgagccagt tcgcggtcga ctcgacgccg
     4861 ttcacgcagt gcgtggcggt ctcgacgacg tcggacccga cggggacgtg gtaccggtac
     4921 gcgtatgcgt ggccgaactc gcgcttcccg gactacccga agctgggcgt ctggccggac
     4981 gggtactaca tcacgacgaa tgacttcaac tcctcgctga cgtcgtttgt gggcgtgacg
     5041 gtcggcgcgt tcgagcgcga caagatgctc gtgggcgacc cggcggggtt catcttcttc
     5101 cagacgtccg gggaccggga gtactcggtg cttccggcgg acctcgacgg catgacgcct
     5161 cctccggccg gggcgccggg gctgatcggc gagttccagg actcgacgtg gatcgggacg
     5221 acgaacgacc agatctggct gttcgagctg aacctggact ggacgaaccc ggcgaactcg
     5281 acgttcggcg tgggagccac ccacgacccg aactgggcga tcgacatctc gaacgtgagc
     5341 gcgaactgct ccggtcgtga ctgcgtgccg cagggaccgt cgagccagaa ggtcgacgac
     5401 atcgacttcc gtctcatgca ccgcctggcg tacacgaacc tggggtcgtc ccaggcgctg
     5461 gtggcgagca tgacggtcgg agcgggcggc cgcgcgggcg tgtggtgggg cgagctccag
     5521 tcgccgaacc cgggtccgac gtggacgctc cggcaggacg gcgtgtacgc gccggccgac
     5581 ggcgaccacc ggtggatgcc ggcgatcgcg atggacatca acgggaacat catgctcggc
     5641 tactcggcct cgtcgacgac ggtgtacccg tcggttcgtt acgtgggccg gctggcgggc
     5701 gacccgctcg ggcagatggg cgacgagaag gtcctcgtcg tcggctccgg ggcgcagagc
     5761 ggcggcaacc ggtggggcga ctactcgatg atgtcggtcg acccgacgga cggctgcacg
     5821 ttctggtaca cgcaggagta catcgcggcg agcggcaact acgactggga cacgtacatc
     5881 gggagcatga agttcccgtc gtgcactgcg ggcccggccg gagtgatcga agggtacgtc
     5941 aggagcacgg cgaacaactc tccgatcgcg ggcgcgagcg tgcagatcgg gggttcgtac
     6001 tcgacgtcga cgaacgcgtc ggggttctac cagctgacgg tgccggtggg gacctacgac
     6061 gtgacggcgt cgaagttcgg ctacggcccg gcttcgatcg cggcgcagga ggtcacggaa
     6121 ggcgccacga cggtcgtggc ggacctgctc ctttcgccgg tcgggtcgta cgcgatcgac
     6181 ggcttcgtca ccgcagaggc ccacaactgg ccgctgtggg cgaaggtcga ggtccggcag
     6241 aacgggacgc tcgtggagac cgtgtacacg tcgccgtgga acgggtacta cgagatcccg
     6301 gccctgccga acgggttcac ctacgacttc acggtcacgt ccatgtacgt gggctaccag
     6361 ccggagaccc gtgtggtgac gatcgcctcg ggcgaccaga tccagagctt ctccctcctc
     6421 gcggcttcgg ggaaccccgc ctacaactgc gtgctgcagg gcgggatcaa cgaggacttc
     6481 aacgctgact tcccgccgct cggctggacg gtcaagaaca acacgtccaa ctcgggcaac
     6541 acctggaagc gtaacgacgt ctggggccgc gcgaacctga cgggcggtac cggcacggcc
     6601 gcggccgcgg actccgacaa ggcgggctcc ggctccggga cgttcgacac ggagctctgg
     6661 tccccggtca tccagatgcc ggcggagccg aggaacctcg ttttccggag cgcctacacc
     6721 cagtacaccg gccagacggg gacgctcgac gtctccacga acggcgggaa cacctggacc
     6781 aacctcctca cgatcacgac gagcgccacc gg
//
LOCUS       DAOONL010000159         6729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13506_length_6729_cov_19.588852, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000159 DAOONL010000000
VERSION     DAOONL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13506_length_6729_cov_19.588852"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PLP50_15410"
     CDS             <1..1317
                     /locus_tag="PLP50_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPA52979.1"
                     /translation="PVADVNNDPDNPVINVRSFGEQPEAVGRFVAATVAGLQEGGVLA
                     TLKHFPGHGDTNVDSHRSLPVLGVDRARLDAVELVPFRAGLAAGARGVMTAHLAVPSL
                     DPEPAPVLAAPIRTKDFTAEVTEVQTAGTMPAVVSRPITTTLLREELGFRGLVFTDSM
                     EMGGIVAHFEPGEAAVRVLEAGADVVLMPPDPRVAIDAIVSAVETGRLPASRLDEAVA
                     RILAEKERLGLFRERSLPLHEIARAAGTREQKATEEEVARRGLTLVREAKGALPLREG
                     SRLLVVAVHDERTTVAIDAPLQAELKRRAASVDFVRLDPTSCAGEGAAAAERAKEADA
                     VLFAFFVRPRSGRGSLEIPPEGKAALAPLFASGRPVIGVSFGSPYLLRDLPGLPTYLC
                     AYGHQSLVQAAAVRALWGEAPIEGTLPVTIPGIAPRGMGIRKEAVK"
     gene            1314..>1540
                     /locus_tag="PLP50_15415"
     CDS             1314..>1540
                     /locus_tag="PLP50_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52980.1"
                     /translation="MNPLGDAPRRPGRPVLFAALLTVLLPTPRALAAPRPLETLSPEA
                     VGLSSARLARIDALLAEAIARKEVPGGVVLVG"
     assembly_gap    1541..1640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1641..3922
                     /locus_tag="PLP50_15420"
     CDS             <1641..3922
                     /locus_tag="PLP50_15420"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPA52981.1"
                     /translation="LAEAIARKEVPGGVVLVGRHGKVAFRRAYGHRSLEPSVEPMTTD
                     TAFDLASLTKGVATAASVMTLVEQGRIALDDPVVRYLPEFGAGGGERERVTVEQLLTH
                     RSGLVADDPMSLYEGTPAEIYARKHRTRLATPPGLRFVYSDAGYEVLGELVRAVTGET
                     LDVYARRAVFEPLGMRETEFRPAGRGGRLPVGRIAPTEKRDGVFLRGEVHDPRARALG
                     GVAGHAGLFGTADDLARFARALLGEGDGWLSPAGIAATTRVRDYGDGDLRALGWDVET
                     HYSTSRGDLFPLGSFGHTGWTGTSMWLDPVTDSFVILLTARNHPGGAGNAIPLRTRLA
                     SVVAAAIEDVPADAFRRASERLAVLTGRPSAPLPPAPQAAAEALSYDVRAGVDVLEST
                     GFEAIAGRRVALLTNATGVTRDGRTTLSVLRSPRAQSAGVTVVRLFSPEHGLAAALDE
                     KVGDSVEPTSGLSVVSLYGEKRRPAPEDLAGLDALVVDLQDAGVRFYTYLTTMAYAME
                     ECAKGKVAVVVLDRPNPIAGLAVEGPPADADRLSFVAVHTIPIRTGMTIGETARMLNV
                     ERGIGADLAVVPLRGWRRALWYDETGLPWVNPSPNLRSVTQAALYPGVALLETTNVSV
                     GRGTDAPFELVGAPWIDAERLARTLNVRAIPGVRFAPVTFTPASSTHAGTLCRGLRIT
                     LTDRSALRPVALGLELATALRDLHGPDWKRDRFGELLGSAAAVTRFERGDPAGTIVAG
                     WAAEQMEFERRRARHLLYE"
     gene            4007..4621
                     /locus_tag="PLP50_15425"
     CDS             4007..4621
                     /locus_tag="PLP50_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52982.1"
                     /translation="MAETRPASGRARSKKEEEYFLRAEAELLEKVRVRAAKEAERKAM
                     GEQHGVEDPEILAAFEEAGYDRHTVRILHLVPILQIAWVDGEISNAEREEILRLAAAR
                     GVDEGSAAHARLLSWLDSPPSPEFFERTMTILARLIEALPEDREVALQGEIMTSALKV
                     ASASGGFLGLGNKVSSEEKALLAHFAESFEKAHRAALEKVAKKG"
     gene            complement(4784..4930)
                     /locus_tag="PLP50_15430"
     CDS             complement(4784..4930)
                     /locus_tag="PLP50_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52983.1"
                     /translation="MTSAAWAMLVVTWSVILYFAIRFFLKVLRNPTPPEPGEDGPEIP
                     FKDA"
     gene            complement(4930..6567)
                     /locus_tag="PLP50_15435"
     CDS             complement(4930..6567)
                     /locus_tag="PLP50_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPA52984.1"
                     /translation="MTAQKEQWASRAGLVLAMAGNAVGLGNFLRFPAQAAQNGGGVFL
                     IPYLVAFVLMGIPLLWVEWAIGRHGGQYGHHSAPGMLDRLGKSRLLKYIGVFGLFTNL
                     TVAAYYCYIESWTLAYVFHSITGTFRSTPPTEFFPAYLGQHAGSITALPPEATLFFVV
                     TLALNVWILSRGLAKGIEMAAKIGVPLLIVFGLILVVRGLTLGTDAPGVVESPLAGLN
                     FVWNPDLSGLKDFNVWLAAAGQIFFTLSVGMGSIHCYAAYVRKNRDIALNAASAGWMN
                     EFVEVVIGGSLLIPIATAYLGLAAVKGATAGGSGFSMGFLTLPSLFNNWGWFAPVAGA
                     MWFGLLFFAGITSSLAMGQPILAFLADEFGAARAKGALLFGGCTFVLGMWCVWLYPGG
                     SFDEFDFWTGTFSLVVFALLEVIVFAWIFGMEKGWAEITRGADMKVPVVFRYVIPYVT
                     PLFLGVVFLGALVQPAGGDWGEAVRGLLDGKGWSLSAGSVLGKLMHVGDTGGWHGPDG
                     EVTPRMVKDLTRILLSLVFLTCGVLVFLAWRRKERRA"
BASE COUNT          977 a         2512 c         2251 g          889 t
ORIGIN      
        1 ccggtcgccg acgtcaacaa cgaccccgac aacccggtca tcaacgtccg gagcttcggc
       61 gagcagccgg aagcggttgg gcgcttcgtc gcggccaccg tcgcggggct ccaggaagga
      121 ggcgtcctcg cgacgctgaa gcacttcccg gggcacggcg acacgaacgt cgactcgcac
      181 cgctcgctcc ccgttctcgg tgtcgatcgc gcccgcctcg acgcggtcga gctcgtcccg
      241 ttccgggccg ggctcgccgc cggggcgcgg ggcgtcatga cggcccacct cgccgtcccg
      301 tcgctcgacc cggagccggc gcccgtcctc gccgccccga tccggacgaa ggacttcacg
      361 gcggaggtca ccgaggtgca gacggccggc acgatgcccg ccgtcgtctc ccgcccgatc
      421 acgacgacgc tcctccgcga ggaactcgga ttccgcggcc tcgtcttcac cgactcgatg
      481 gagatggggg ggatcgtcgc ccacttcgag ccgggggagg ccgccgtccg ggtcctcgag
      541 gcgggggccg acgtcgtcct gatgccgccc gacccgaggg tcgccatcga cgccatcgtc
      601 tcggccgtcg agacgggccg cctgcccgcc tcccgtctcg acgaggccgt cgcccggatc
      661 ctcgccgaga aggagcggct cggcctcttt cgcgagcgga gccttcctct ccacgagatc
      721 gcccgggcgg ccggcactcg ggagcagaag gcgaccgagg aggaggtcgc gcgccgcggt
      781 ctgaccctcg tccgcgaggc gaagggagct ctcccgctcc gcgagggctc acggcttctc
      841 gtcgtcgccg tccacgacga gcggacgacc gtcgcgatcg acgcgccgct ccaggccgag
      901 ctgaagcggc gcgcggcctc cgtcgacttc gtccgcctcg acccgacctc ctgcgccggc
      961 gagggagccg cggccgccga gcgcgcgaag gaggccgacg cggtcctctt cgcgttcttc
     1021 gtccggcccc gctccggccg gggctccctc gagatcccgc cggaggggaa ggccgcgctc
     1081 gcgccgctct tcgcgtcggg ccggcccgtc atcggcgtct cgttcggaag cccctacctg
     1141 ctccgcgacc tccctgggct cccgacttat ctctgcgcct acggtcacca gtccctcgta
     1201 caggcggcgg ccgtccgcgc tctctgggga gaggccccga tcgaggggac gctccccgtc
     1261 acgatccccg gcatcgctcc gcgcggcatg gggatccgaa aggaggccgt gaagtgaatc
     1321 cgctcggcga tgctccgcgc cggcctggcc gccccgtcct cttcgcggcc ctcctcaccg
     1381 tccttctccc gacccctcgc gcgctcgcgg cgccgcgtcc cctcgagacg ctgtccccgg
     1441 aagccgtcgg cctttcgtcg gcccgcctgg cgcgcatcga cgcgctcctc gccgaggcga
     1501 tcgcacgcaa ggaggtcccg ggcggcgtcg tcctcgtcgg nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn tcctcgccga ggcgatcgca cgcaaggagg tcccgggcgg
     1681 cgtcgtcctc gtcggccgac acggaaaggt cgcgttccgc agggcgtacg gccaccggtc
     1741 gctcgagccc tccgtcgagc cgatgacgac ggacaccgcg ttcgacctcg cttccctcac
     1801 gaagggggtc gccaccgccg cgagcgtcat gaccctcgtg gagcaggggc ggatcgcgct
     1861 cgacgacccg gtcgtccggt acctgcccga gttcggcgcc ggcggcgggg agcgggaacg
     1921 cgtcacggtc gagcagctcc tgacccatcg gagcggcctc gtcgccgacg acccgatgtc
     1981 gctctacgag gggactccgg ccgagatcta cgcgcgcaag caccgcaccc ggctcgcgac
     2041 gccgcccggg ctccgcttcg tctactccga cgccggctac gaggtcctcg gcgagctcgt
     2101 ccgggccgtg acgggcgaaa cgctcgacgt ctacgcccgc cgcgcggtct tcgagccgct
     2161 cgggatgcgc gagaccgagt tccggcccgc cggacgggga gggcggcttc ccgtcggccg
     2221 gatcgcaccg accgagaagc gggacggcgt cttcctccgc ggagaggtgc acgacccgcg
     2281 cgcgcgggcc ctcggtggcg tcgccggcca cgccggcctc ttcggcacgg cggacgacct
     2341 cgcccgcttc gcccgagccc ttctcggtga gggagacggc tggctctccc cggctgggat
     2401 cgccgcgacg acccgcgtcc gcgactacgg ggacggggac ctgcgggcgc tcgggtggga
     2461 cgtcgagacc cactactcga cgagccgcgg cgacctcttc ccgctcggct ccttcgggca
     2521 caccgggtgg accgggacct ccatgtggct cgaccccgtc accgacagct tcgtcatcct
     2581 cctcaccgcg cgaaaccacc ccggcggcgc cggaaacgcg atccctctcc ggacgcggct
     2641 cgcgagcgtc gtcgctgcgg cgatcgagga tgtcccggcc gacgcgttcc ggcgcgcctc
     2701 ggaacgactc gccgtcctga cgggccgccc ctccgcacct cttcctcctg cgccgcaggc
     2761 ggccgccgag gccctctcgt acgacgtccg ggccggggtc gacgtcctcg agtccaccgg
     2821 cttcgaggcg atcgcggggc ggcgcgtcgc gctcttgacg aacgcaacgg gggtgacgcg
     2881 cgacgggcgg acgacgctct ccgtcctccg ctcgccgcgc gcacagtccg ccggcgtgac
     2941 ggtcgtccgc ctcttctctc ccgagcacgg cctggcggcc gccctcgacg agaaggtcgg
     3001 cgactcggtc gagccgacct ccggcctttc ggtcgtctcg ctttacggcg agaagcgacg
     3061 cccggcaccc gaggacctcg caggcctcga cgcgctcgtc gtcgacctcc aggacgccgg
     3121 cgtccgcttc tacacctacc tcacgacgat ggcgtacgcc atggaggagt gcgcgaaggg
     3181 caaggtcgcc gtcgtcgtcc tcgaccggcc gaacccgatc gcggggctcg ccgtggaggg
     3241 gccgccggcc gacgccgacc ggctctcctt cgtcgccgtc cacacgatcc cgatccggac
     3301 cgggatgacg atcggcgaga ccgcccgaat gctgaacgtg gagcggggga tcggcgccga
     3361 cctcgccgtc gtcccgctcc gcggctggcg ccgcgcgctc tggtacgacg agacgggcct
     3421 cccctgggtg aacccctctc cgaacctccg ttccgtcacg caggcggcgc tctacccggg
     3481 cgtcgccctc ctcgagacga cgaacgtctc cgtcggtcgc gggaccgacg cgccgttcga
     3541 gctcgtcggc gccccgtgga tcgacgccga gcgcctcgcc cggacgctga acgtccgggc
     3601 gatcccgggc gtccgcttcg ccccggttac gttcaccccc gcctcctcca cgcacgccgg
     3661 caccctctgc cgcgggctcc ggatcacgct caccgaccgg agcgcgctcc gccccgtggc
     3721 gctcggcctc gagctcgcga cggcgctccg cgacctccac gggccggact ggaagcggga
     3781 ccgcttcggc gagctcctcg gcagcgccgc ggccgtgacg cggttcgaga ggggcgaccc
     3841 ggccgggacg atcgtcgccg gatgggccgc cgagcagatg gagttcgagc gccggagggc
     3901 ccgccacctc ctctacgagt agcccggcga ccctcgccgt acctgccgac gttctggtaa
     3961 aaccccgccg cggattcaga cccgagattc gacgaggagg acccagatgg ccgagacccg
     4021 ccccgcttcc ggtcgagcac gctccaagaa ggaagaggag tacttcctgc gggcggaagc
     4081 cgagctgctc gagaaggtgc gcgtccgcgc ggcgaaggag gccgagcgga aggcgatggg
     4141 cgagcagcac ggcgtcgagg accccgagat cctcgcggcg ttcgaggagg ccgggtacga
     4201 ccgacacacg gtccggatcc tccacctcgt cccgatcctg cagatcgcct gggtcgacgg
     4261 ggagatctcg aacgccgagc gcgaggagat cctccggctc gccgcggccc gcggcgtcga
     4321 cgagggaagc gcagcccacg cccgccttct ctcctggctc gactctcccc cgtccccgga
     4381 gttcttcgag cggacgatga cgatcctcgc ccgcctcatc gaggccctcc cggaggaccg
     4441 ggaagtcgcc ctccagggcg agatcatgac ctccgccctg aaggtcgcct cggcgtcggg
     4501 cggtttcctc gggctcggca acaaggtctc gtcggaggag aaggccctcc tcgcccactt
     4561 cgccgagagc ttcgagaagg cccaccgggc cgcgctcgag aaggtcgcga agaagggctg
     4621 agtccccgct tccccggaca ccgacgcgcg agctgaggca ggtcggcgaa cccggcgcca
     4681 cgaactcggt gccaggcgtg aaatcgtgta ttgttttcaa caatacacga tttcacgcct
     4741 ggcaccctcg cgaggggcag cctcgcgagg cccgcccgtc gcgtcacgcg tccttgaagg
     4801 ggatctccgg cccgtcctcg cccggttcgg gcggcgtcgg gttccggagg accttcagga
     4861 agaagcggat cgcgaagtag aggatgaccg accaggtcac caccagcatg gcccaagcgg
     4921 ccgaggtcat tacgccctcc tttccttccg ccgccaggcg aggaagacga ggactccgca
     4981 ggtcaggaag acgagcgaga gcaggatccg cgtcaggtcc ttgaccatcc gcggcgtcac
     5041 ctcgccgtcg ggcccgtgcc agccgcccgt gtcgccgacg tgcatcagct tgccgaggac
     5101 gctcccggcc gagagcgacc accccttccc gtcgaggagg ccgcggaccg cctcgcccca
     5161 gtcccctccc gcgggctgga cgagcgcccc gaggaagacg actccgagga agagcggcgt
     5221 cacgtagggg atgacgtacc ggaagacgac ggggaccttc atgtcggccc cgcgcgtgat
     5281 ctcggcccac cccttctcca tcccgaagat ccaggcgaag acgatcacct cgaggagcgc
     5341 gaagacgacg agcgagaacg tcccggtcca gaagtcgaac tcgtcgaacg agccgcccgg
     5401 gtagagccag acgcaccaca tcccgaggac gaacgtgcag ccgccgaaga ggagcgcccc
     5461 cttcgcgcgc gcggcgccga actcgtccgc gaggaacgcc aggatcggct gccccatggc
     5521 gagcgacgag gtgatgccgg cgaagaagag gaggccgaac cacatcgcgc ccgcgaccgg
     5581 cgcgaaccag ccccagttgt tgaagaggct cgggagcgtc aggaacccca tcgagaaccc
     5641 gctcccgcca gccgtggccc ccttcacggc cgcgaggccc aggtacgccg tcgcgatcgg
     5701 gatcaggagc gacccgccga tgacgacctc gacgaactcg ttcatccagc ccgcgctggc
     5761 ggcgttcagg gcgatgtccc tgttcttcct cacataggcc gcgtagcagt ggatcgaccc
     5821 catgccgacc gagagcgtga agaagatctg ccccgccgcc gcgagccaga cgttgaagtc
     5881 cttgagcccc gagaggtccg ggttccagac gaaattgagg cccgcgagcg ggctctcgac
     5941 gacgcccggg gcgtccgtgc ccagtgtgag cccacggacg acgaggatga gcccgaagac
     6001 gatgaggagc gggacgccga tcttcgccgc catctcgatc cccttcgcga ggccgcggga
     6061 gaggatccag acgttcaggg cgagcgtgac gacgaagaag agcgtcgcct ccggcgggag
     6121 cgcggtgatc gacccggcgt gctgtccgag gtaggccggg aagaactccg tcggaggcgt
     6181 cgaccggaac gtccccgtta tcgagtgaaa gacgtaggcg agcgtccacg actcgatgta
     6241 gcagtaataa gctgccaccg tcaggttcgt gaagaggccg aagacgccga tgtacttcag
     6301 gagccggctc ttccccagcc ggtcgagcat cccgggggcg gagtggtggc cgtactgccc
     6361 gccgtgccgg ccgatcgccc actcgaccca gaggagcggg atccccatca ggacgaacgc
     6421 gacgaggtag gggatcagga agacgccccc gccgttctgc gcggcctggg ccgggaagcg
     6481 caggaagttg ccgagcccga cggcgtttcc ggccatcgcg aggacgagcc ccgccctcga
     6541 agcccactgt tccttctgtg ccgtcatggg caccccctct gccgatccgg cagggtagcc
     6601 gcgcccggga gggggcgtca acgcgagaaa cgggctcatc gcccccccgc ccgcgcgaga
     6661 atccgtcccc atgaaggtcg ccctcctcca gctcgacctc gcctgggagg acgtcccggc
     6721 gaaccacgc
//
LOCUS       DAOONL010000160         6713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13543_length_6713_cov_14.419195, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000160 DAOONL010000000
VERSION     DAOONL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13543_length_6713_cov_14.419195"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6713
                     /locus_tag="PLP50_15440"
     CDS             <1..>6713
                     /locus_tag="PLP50_15440"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPA52985.1"
                     /translation="ETPVSDCNPEPAFLHGLSAPPLAPALAGPVTVVSAQTVDVGTVS
                     LGAAGTITGTVVAAEYGYPVSSAVVTVPGTASEAITNYAGAFTLHVAPGSASVTATKA
                     GWSTATVGPFAVVAGSSIAAGAIPLTRWGAVTGRVTADYDDSPIEGVTVTLSGGGGTG
                     MTDSTGTFTISCAPGTYTATYAKAGFETAVSWEFTTWSGGTYTLWDTRLVRHGVATGL
                     VTSSADGQPLSGVHVAVLGGSASTNTGSDGRFAITVPPTPTRLVLSKAGFGTALTGEL
                     MVGIGESTDVGALSLGAGGVLSGIVVSAGTGAPLAGVLVSVQPEVETSLTDASGAFSF
                     ELAPGDYSVVLSREGWDSSAAGPFSITAGATTDVGALPLTELGTIVGTVVRAPEGTPV
                     AGAIVRMVGAPGTVTTDATGHFSIKARPGAVTLLIESPPYSATAAGPVTVVSAQTVDL
                     GVVQLLPPGTLRGTVVGGLSNVPVGGATVRLNGASDIATTDSLGAFSFQLAAGTVNLT
                     ISKSGFTTLTTGSVTVTSGATTDMGPVHLVQNGSIVGRVASSAGGVPLEGATVRLEDP
                     RRTATTAADGSFVLSGIPAGTVGYTVAVSLEGFSTSSAWPRLVFEGQATDLGTILLDP
                     PGEVYGTILGSVISDATGMPVPGANVHVWRSSGAIAVSGTTDATGAFSLSVPVSTGLS
                     VQATTTGLASMPVTEVAVAGWGTKDLGTLRLVPAGVISGKVLASDACTVCAKDTGWCT
                     RTEALDGQFALQAPPGTWDVVAWYRPERPTTKGPVSVVAGETVSVGEIRVETARARNV
                     TGKAVGALSGAPLADGSAALSNSALPPDSIWSGEFGFYDVPPGSYAAIVSRPGWLPAT
                     GPSFTVVAGSVADAGKTALERGGKVLGTVRDSLSGLPLANADVLVSGTGYSDRTDEYG
                     GFLLSVPAGAHSLRILKEGWQTTLAGPTSVAVGSVVNLGYIDLVPESTAPGLVTGTVE
                     TAQGPAVGSRVQVVPGGASVLTDAQGAYSLSVPAGTVSIIASLDGWASAGRSGVVVLA
                     GQTVPLEDLTLSEPAAAVSGRVSSMYGGAPIAGAVVTVDGTGVAGLTDATGAFSLLVT
                     PGIAVVRVVATGWGYTESGYNGVRRLVLAPGQVASGIVLTLEPLSEVSGRIVDSVDGA
                     PIAGARISTSASSTETDADGRFRTWEFAGPGGHCGVTKTGWSRSGDHFDVSPGQPVAL
                     GDLQAVGTGTLVGMVGWDHMQMNPSPVATVVVEGAVGSYQTDADGVYSITVPPGTYTV
                     RASRSSSYDTVTSAPVSVVSGETMEVERIYLAGYGAAYGTVRDADTQLPLGGVTVSAL
                     ETGASTVSDSAGSFSLRLRAGGYTIVFTKPGYVTRTATAVHIGSTLGGAFILGVDLTP
                     ESTFELAQLAIVPSYVRSFETATGTVIAKGPAPSGGATVTLISDNPSAVTMPATVSFL
                     EGESTASFLITGSYPPAAQNVKITARHQKGLGWTETWAYVSVDPPAPIVLLGLSPATA
                     LPGDGHVVGYVESLTASDYTVSLAGPVYALDSLSAPLCDLASGACPTQSLPAPLEPGD
                     TAVRFALPEGLGEGYYAVRVVEATGRSTSWGWMGVEARQRSIALQSPDEHRSARRLLP
                     GQVVTGTFAENGDPNGNLGDYNFFYFIAPAGSVVNARLERVDTSLPWEAPSSLDPQLE
                     LIAPDGFIYANVRRFDDAAGSDLNATLTNAVLPQGGLWLLAAETTRGSGDYRLTFDLT
                     PAPPAAGERAVPISGNGNTLPLGVPLTSAAIVLDPRGYPLSGATVTFAPSTAPENKGI
                     LQFASGNTVTSGPDGSAIVTATFTSPGKASLTPTFSLPLFANLVSDSPAMKAKAEIPI
                     YEPVGRWPVAVRDFSEDTVELRPAMRQEAPPSIRGLRGESDTTPRERTLDAANATPLS
                     VTDSGSGRVAALERISGCQAGTFNNAGVVAAEVHAPFTLSLEDLTPKPGESAPPGVLD
                     TTEGINGHRIERTVRLRLDIKDATESAPTHPVLVQMAVGGPKAGKLILDPDGARIECT
                     SVSFLWHDRDAQGKPAEKNEIVEYRLGEYAPLVGFKPDEQAPGTVEPVWGVAEQLKIV
                     LSAPDESGSETWERTLDVSYPVRPQPGKPDHFDCIDYNGDPCPDVFAFWTGYRLYQSG
                     TRADGTPRLITEPFTITNTYVLADARGNTAWGYRNVSATDPSPSLTVGFRDQATTGPD
                     FAPYALSVSWSNDPAFPQGSIPATLSVQYPTDPEWPAGTVEKVITLD"
BASE COUNT          992 a         2379 c         2301 g         1041 t
ORIGIN      
        1 tggagacacc agtctccgat tgcaaccccg agccggcatt tcttcacggg ctctcggctc
       61 cacctctcgc cccggcgctc gcggggccgg tcacggtcgt ttccgcccag accgtcgacg
      121 tcgggaccgt ctcgctcgga gccgcgggga cgatcaccgg cacggtcgtc gccgcggagt
      181 acggctatcc cgtctcgtcc gccgtcgtca cggtgcccgg gacggcgagc gaggcgatca
      241 cgaactacgc gggagccttc acgctccacg tcgcgcccgg ttcggcctcg gtcacggcga
      301 cgaaggccgg ctggtcgacg gcgaccgtcg gtcccttcgc cgtcgtcgcg ggcagctcga
      361 tcgcggccgg tgcaatcccg ttgacgcggt ggggtgccgt gacggggcgg gtcaccgccg
      421 attacgacga cagcccgatc gagggcgtca cggtgaccct ctcgggaggc ggcgggaccg
      481 ggatgacgga ctcgaccggc accttcacga tctcgtgcgc accggggacg tatacggcca
      541 cctacgcgaa ggccgggttc gagacggccg tctcctggga attcacgacg tggtcgggcg
      601 ggacgtacac gctttgggac acgcgcctcg tgagacacgg ggtggccacg gggctcgtga
      661 ccagctcggc ggacggccag cctctttcgg gcgtccacgt cgcggtcctc ggcggatcgg
      721 cttcgacgaa caccgggtct gacggccggt tcgcgatcac agtcccgccg acccccacac
      781 gcctcgttct ctcgaaggcc ggcttcggga ccgccctgac cggtgagctc atggtcggaa
      841 tcggagagtc gacggacgtg ggagccctta gcctcggcgc gggcggcgtt ctcagcggga
      901 tcgtcgtcag cgctggcacc ggcgcgccgc tggcgggagt cctcgtttct gtccagcccg
      961 aggtcgagac gagcctcacc gacgcttccg gagccttctc cttcgagctc gccccggggg
     1021 actactccgt cgtcctgagc agagagggct gggactcctc ggccgccggc ccattcagca
     1081 ttacggctgg tgcgacgacg gacgtcggcg cccttccgct caccgagctc ggaaccatcg
     1141 tcgggaccgt cgtgagggcc cccgaaggaa caccggtcgc gggcgcgatc gtccgaatgg
     1201 tgggtgcccc cggcaccgtg acgacggacg cgacggggca cttctcgatc aaggctcgcc
     1261 cgggcgccgt cactctcctc atcgagtccc ctccgtactc tgcgacggcg gccgggccgg
     1321 tgaccgtcgt ctcggcacag accgtcgacc tcggggtcgt tcagctgctt ccgccgggaa
     1381 cccttcgagg cacggtcgtg ggcggcctgt ccaacgtgcc cgtcgggggt gcgaccgtcc
     1441 ggttgaacgg cgcgagcgac atcgcgacga cggattccct gggcgcgttc agcttccagc
     1501 ttgcggcggg aaccgtgaat ctcaccatct cgaagtcggg attcacgact ctcacgaccg
     1561 gcagcgtcac ggtgacgagc ggcgcgacga cggacatggg tcctgtccac ctcgtccaga
     1621 acggctccat cgtgggtcgc gtggcgagct cggccggggg agtaccgctc gagggcgcaa
     1681 ccgtgcgcct cgaggatcct cggcgaacgg ccacgaccgc ggcggacggc agcttcgtcc
     1741 tctcgggcat tcccgcaggc acggtcggct acaccgtcgc cgtgtcgctc gagggcttct
     1801 ccacatcatc ggcctggccc cggctcgtct tcgaggggca agcgaccgat ctcggcacca
     1861 tcctgctcga cccgcccggc gaggtctacg gaacgatcct cggctccgtc atcagcgacg
     1921 cgacgggcat gccggttccg ggggccaacg tgcacgtctg gcgctcgtcc ggcgcgatcg
     1981 ccgtctcggg gacgaccgac gccacgggcg ccttttcgtt gtccgttccc gtgtcgacgg
     2041 gtctctccgt gcaggcaacg acgacggggc tcgccagcat gcctgtcacg gaagtggcgg
     2101 tggcgggctg gggaacgaag gacctgggca cgctccgtct cgttccggca ggtgtgatct
     2161 ccgggaaagt gctggcgagc gacgcctgca ccgtctgcgc gaaagacacg ggctggtgca
     2221 cgaggacaga ggccctggac gggcagttcg cgcttcaagc ccctccggga acctgggacg
     2281 tcgtcgcgtg gtaccggccc gagaggccca cgacgaaggg tcccgtgtcc gtcgtcgccg
     2341 gcgagacggt gagcgtcggc gagatcagag tcgaaacggc gagggctcgg aacgtcacag
     2401 gaaaggccgt tggcgcgctc agcggcgcgc cgctcgccga cgggtccgcg gcgctgtcca
     2461 acagcgcgtt gccgccggac tccatctggt cgggcgaatt cggcttctac gacgtgcctc
     2521 cgggttcgta cgcggcgatc gtctcgaggc ccggatggct gccggcaacc ggtccgtcgt
     2581 tcaccgtggt cgccgggagc gtggccgacg cggggaagac cgcgctcgag cgtggcggca
     2641 aggttcttgg aaccgttcgg gactcgctct ccggcctgcc gctcgcgaac gcggacgtcc
     2701 tcgtctcggg cactgggtac tccgaccgga ccgacgagta cgggggtttt ctgctttcgg
     2761 tcccggcggg tgcgcactcg ctgaggatcc tgaaggaggg ctggcagacg accctggcag
     2821 gacccacctc ggtcgccgtg ggatcggtcg tgaacctcgg ctacatcgac ctcgtgccgg
     2881 agtcgacggc tccggggctc gtgacgggta cggtcgagac cgcccagggg ccggccgtcg
     2941 gctcgcgcgt gcaggtcgtc ccggggggcg catccgttct gaccgacgcg cagggcgcgt
     3001 actccctgag cgtgccggca gggacggtgt cgatcatcgc gagtctcgat ggctgggcct
     3061 ccgcgggtcg aagcggcgtg gtcgttctgg ccgggcagac cgtccccctg gaagacctca
     3121 ccctctcaga gccggcggct gccgtttccg ggcgggtctc gagcatgtac ggtggggcgc
     3181 cgatcgctgg ggcggtcgtg accgtcgatg gaacgggcgt cgcgggtctg acggacgcga
     3241 cgggagcctt cagtctcctg gtgacgcccg ggatcgcagt cgtccgggtg gtcgcgacgg
     3301 gctgggggta cacggagtcg ggatacaacg gtgtccggcg cctcgttctg gcgccgggtc
     3361 aggtcgcgtc cggaatcgtc ctcacactcg aaccgctctc ggaggtctcg gggcggatcg
     3421 tcgactccgt cgacggtgct cccattgccg gggctcgaat ctccacctcc gcgtcctcca
     3481 cggagacgga tgcggacgga aggttccgca cgtgggagtt cgccggtccc ggcggccatt
     3541 gcggggtgac caagacggga tggtcgcggt caggagacca cttcgacgtc tcgccggggc
     3601 agcctgtagc cctcggagac ctgcaggcgg tgggcaccgg gacgctcgtc ggcatggtcg
     3661 gatgggatca catgcagatg aaccccagcc cggtcgccac cgtcgtcgtg gagggagcgg
     3721 tggggtcgta ccagaccgac gccgatggcg tgtacagcat caccgttccg ccagggacct
     3781 acacggtccg ggcgtcccgg tcctccagct acgacaccgt gacgtcggcg ccggtctccg
     3841 tcgtgagcgg cgagacgatg gaggtggagc ggatctacct cgcggggtac ggagccgcgt
     3901 acgggacggt tcgggatgcc gacacgcagc tccccctggg gggcgtcacc gtgtccgctc
     3961 tcgagaccgg ggcgagtacg gtcagcgact cagccggaag cttcagcctg cggctccgtg
     4021 cggggggcta cacgatcgtc ttcacgaagc cggggtatgt cacgcggacc gcgacggcgg
     4081 tccacatcgg atccactctc ggcggcgcgt tcatcctcgg tgtcgacctg acgccggagt
     4141 cgaccttcga gctggcacag ctcgccatcg tgccgagcta cgtccgctcc tttgaaacgg
     4201 cgaccggaac cgtgatcgcg aagggcccgg caccgagcgg cggagcgacg gtgaccctca
     4261 tcagcgataa cccgtccgcg gtcacgatgc cggcgaccgt ctcgtttctc gagggcgaat
     4321 cgacggcgtc gttcctcatc accgggagtt atccgccggc cgcgcagaac gtgaagatca
     4381 cagcgaggca ccagaagggt ctcggctgga cggagacctg ggcctacgtc agcgtcgatc
     4441 caccagcccc catcgtcctg ctcggtcttt ctcccgccac ggccctcccg ggcgacggac
     4501 acgtcgtcgg gtatgtcgag agtctgacgg catccgatta caccgtctcg ctggccggac
     4561 ccgtctatgc cctcgacagc ctctccgcgc ccctctgcga cctcgcctcg ggcgcctgcc
     4621 cgacgcagag cctcccggcg cccctcgagc cgggcgacac ggccgtccgg ttcgcgcttc
     4681 ccgaaggcct cggcgagggg tactacgccg tgagggtcgt cgaggccacc ggtcgatcca
     4741 cgtcctgggg ctggatgggc gtcgaggcgc gccagaggtc gatcgcgctt cagtctcccg
     4801 atgagcatcg ttcggcccgg cgcctcctcc ccggtcaggt cgtcaccggc acgttcgccg
     4861 aaaacggcga cccgaacggc aacctgggcg actacaactt cttctacttc atcgcgcccg
     4921 cggggagcgt cgtcaacgcg cgcctcgagc gggtcgacac gtccctgccg tgggaggcgc
     4981 cgtcgagcct cgatccgcag ctcgagctca tcgcgccgga cgggttcatc tacgcgaacg
     5041 tccggaggtt cgacgacgcg gccggctccg acctgaacgc cacgctcacg aacgcggtcc
     5101 ttccgcaggg aggactctgg ctcctcgccg cggagacgac acggggctcc ggggactatc
     5161 gcctgacgtt cgacctgacg cccgcgcctc cggcggcagg ggagagagcc gttccgatct
     5221 cgggcaacgg gaacaccctg cccctcggag tccctctaac ctccgcggcg atcgtcctgg
     5281 acccgcgtgg ctatccgctc tcgggcgcga ccgtgacctt tgccccctcg acagccccgg
     5341 agaacaaggg gatcctccag ttcgcctcgg gcaacaccgt cacgagcgga ccggacggga
     5401 gcgccatcgt cacggcgacc ttcacatctc ccgggaaggc gagcctgacc ccgaccttct
     5461 cgctcccgct cttcgcgaac ctcgtctccg actcgccggc catgaaggcg aaggccgaga
     5521 taccgatcta cgagccggtc ggccgctggc ccgtcgcggt tcgggacttc tcggaagaca
     5581 ccgtcgagct gagaccggcg atgcggcagg aggctccgcc ctccatccgg ggcctgcgcg
     5641 gcgagtcgga cacgactccg cgcgagcgca ccctcgatgc ggcgaacgcc acgccgctat
     5701 ccgtcaccga tagcggtagc ggccgagttg ccgcgctcga gaggatctcc ggctgccagg
     5761 ccggcacctt caacaacgca ggtgtcgttg cggccgaggt ccacgccccc ttcaccctct
     5821 cgctggagga cctcacgccg aagccggggg agtcggctcc gcccggcgtc ctcgacacga
     5881 cggagggaat caacggtcat cgaatcgaga gaaccgtccg cctcaggctc gacatcaagg
     5941 acgccaccga aagcgccccg acccatcccg tcctcgtgca gatggccgtt ggagggccga
     6001 aggccggcaa gctcatcctc gaccccgacg gcgcccgcat cgagtgcacg agcgtcagct
     6061 tcctctggca cgaccgggac gcccagggga agcccgccga aaagaacgag atcgtcgagt
     6121 accgcctcgg cgagtacgcc ccgctcgtcg gcttcaagcc agacgagcag gcgcccggga
     6181 ccgtcgagcc cgtctggggc gtggcggagc agctgaagat cgtcctctcc gcgccggacg
     6241 agtcgggctc ggagacctgg gagcgaacgc tcgacgtcag ctacccggtt cgcccgcagc
     6301 cgggcaagcc ggatcacttc gactgcatcg actacaacgg cgatccctgc cccgacgtct
     6361 tcgccttctg gaccgggtac cgtctctacc agtcgggcac ccgggccgac ggcacgcccc
     6421 ggctcatcac ggagcccttc accatcacca acacctacgt cctggccgac gccaggggaa
     6481 acaccgcgtg gggctaccga aacgtctcgg ccacagatcc ttcgccgagc ctgacggtgg
     6541 gcttccgaga ccaggccacg acggggccgg acttcgcgcc gtacgccctt tccgtgtcgt
     6601 ggagcaacga cccggcattc ccccagggct cgattccggc gaccctctcg gtccagtacc
     6661 ccacggaccc cgagtggcct gccggaaccg tggagaaggt catcaccctc gac
//
LOCUS       DAOONL010000161         6681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13638_length_6681_cov_17.505282, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000161 DAOONL010000000
VERSION     DAOONL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13638_length_6681_cov_17.505282"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..703)
                     /locus_tag="PLP50_15445"
     CDS             complement(437..703)
                     /locus_tag="PLP50_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52986.1"
                     /translation="MNPVAGHRDGVATTGAESGLDGGRGVDTTLARGENPELELALAG
                     GNLNGEGKGVARLRRNDGACEELLLEPMPEEGVAIGDADDGDGE"
     gene            1302..1565
                     /locus_tag="PLP50_15450"
     CDS             1302..1565
                     /locus_tag="PLP50_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52987.1"
                     /translation="MTLVQTSTSVTGEVFASEGRRFPVSSLDGITLHVSGLEGSSTCS
                     TYAFFISDRQYDGHGTLVAFSGNLSGRCCGTLAGPFRVQRRPQ"
     gene            2042..2545
                     /locus_tag="PLP50_15455"
     CDS             2042..2545
                     /locus_tag="PLP50_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52988.1"
                     /translation="MTTFITESPWNRARPRVLVVACSDGRLQENLDDFLQRSLRITHY
                     DRLYAPGGAGALTRSGSDFIRAGFYRRECLDLVRLHGIQSLILLFHGPAIDGPAEAVC
                     ADYRRKLPASSLRDIRRQQEVDARELRSAGLGWPMPVETLVFRCEVGADDVVRFAALA
                     AEPPQIA"
     gene            2757..2966
                     /locus_tag="PLP50_15460"
     CDS             2757..2966
                     /locus_tag="PLP50_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HPA52989.1"
                     /translation="MPETPKAKGPSSHFPFIEKTYGKPISLCMGVLERARPVKHMELV
                     SLLKTEHGLGHGHANALVAHVLATE"
     gene            3091..4104
                     /locus_tag="PLP50_15465"
     CDS             3091..4104
                     /locus_tag="PLP50_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPA52990.1"
                     /translation="MSIATTADTPHDLLAVLRQRLVLLGRSPRTVDAYANWVGRFFRF
                     HPRRAPSELGMGDIESFLTHLATVDHVAASTQGQALAAILCFFREVRSIDPSTPLQFG
                     RARHTRHIPTVLSQREALALLGCLQGTLRLICQVLYGSGLRLSECLNLRVQNLDFDHH
                     TIVVINGKGERDRLTVIPPSLERPLETHLRRLWALHQEDLLRGYGRVPLPNAFARKAP
                     TAACNFRWQYLFPGSRLIDDPRTGLRGRWHLDPSVVQRAVRQAALRAGLRKRVGPHTL
                     RHSFATHLLERGCDLRRIQSLLGHAHLETTAIYTHITDLASASIPSPLEPAVVVASSS
                     PKA"
     gene            4573..5019
                     /locus_tag="PLP50_15470"
     CDS             4573..5019
                     /locus_tag="PLP50_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52991.1"
                     /translation="MTAIGRLGLLLGLAFAPLVHFGPRPWAQVLVGALVVLALLAAAV
                     LTWRRAGLRLASAGMAVAGLAMALLMRILIRGPDPFAAPPSALIAFGLVLLLATAFLL
                     AQSRTAPELWDQWRRHQRTVTLADVLCFRQIPRLPGAGPSSPPIRA"
     gene            5218..5508
                     /locus_tag="PLP50_15475"
     CDS             5218..5508
                     /locus_tag="PLP50_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52992.1"
                     /translation="MKLPVRLVSLLASCTPAWAAAAEVLPEAPRSAGNVGASGLWVLA
                     NICFAGMRAQNSPRAGWRIVAFLFGLPGTLVTYFVVAEGSERAYGIDIPRKR"
     gene            5579..6034
                     /locus_tag="PLP50_15480"
     CDS             5579..6034
                     /locus_tag="PLP50_15480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPA52993.1"
                     /translation="MRTTLTALVLCGLGFAGCATRGVAPPLSPEVELSLDDFRRALDE
                     ATPSDPVFDDRDPSVKARLTPPVELHRVQPELSRAARAQSREGNVALACVIERNGSVS
                     NVKVLSATGHGDFTAASVAAVRRWRYTPAKIDGSPVRLLLTVTTSYRVR"
     gene            complement(6152..>6681)
                     /locus_tag="PLP50_15485"
     CDS             complement(6152..>6681)
                     /locus_tag="PLP50_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52994.1"
                     /translation="GGGPTPEQLATKAALVDAHVARAAASHLASRYDEAVAGQAPAEE
                     GTAPSWEAADAYRASDEGLAAVTYLTELGKPVPVDSSFPRAGDIDAVRKATTELVTIV
                     LAPAGTREEFAARVSDAQARLFNAVSALEKGTKNHILIQTRSESNIRMAEYTEILASS
                     RAAGAGSPPESTPAP"
BASE COUNT          852 a         2650 c         2071 g         1108 t
ORIGIN      
        1 cgcagagctg gctcatccga tccccgaggt ccagaccgat cgtcaggctc ctcgccatcc
       61 ccgccgatgc cgtatccgca cccttgatcc ctcccgcccc cttcccctcc cgtgtcctcc
      121 tcgtcgcctc ggtgctacgc tttcccatgg ccggtctcct ctctgggcct cgagcccgtt
      181 gttccttgca gccgattctt tcgaactgcg ccgggagacc ggccttctca ttccatctcg
      241 cttcgctcgc cgctgaacca acggtcaggt ggcactctcg cgtcgtcgcc actcgtctga
      301 tgctcggcga cacagttccc cttccccttt ccgtgttagg gatgctgcta tgccgcaacg
      361 tgacgctctc ctcatcgtcc tcgcgggcct gctacccgtg gcagcctctg ctgccgcagc
      421 tgctccgcag ccgtcctcac tcaccgtcgc cgtcatcggc gtccccgatg gctactccct
      481 cctcgggcat tggctcgaga agcagctctt cgcacgcacc gtcgttccgt cgaaggcggg
      541 cgaccccttt tccttctccg ttcaggttcc cgccggccag ggccaactca agctctgggt
      601 tctcaccacg ggccagcgtg gtgtcaacac cgcgaccgcc gtcaaggcca ctctccgctc
      661 cggtcgtcgc tacaccgtca cggtgaccag cgacggggtt cactccctcc gcgctacctt
      721 ggcgcaggac cccgccaccg acatcgcccg atgccacggc ttgcccgggt cgatgaagtg
      781 caagcggacg ccgttggccc gcgcccagca gtgcatcgcc ctcgacgtga actccggtcc
      841 gttctccacg ccgatgaact tcggcagacc tcgcgtcttc ttcgcctcgt cgagaatgcc
      901 gagtgacccg cacgccggag atcgacgtgt cggtcgggac gtccggactg tcgcgcgttc
      961 cttcgtccac gatcgtgagg cagcgatacc gccgccccgt ggcgagcgtg ctggagatcg
     1021 acgctcgcct tccgctgacg cccgccgtta ggcgccgttc ggacccgtca ctgcccttca
     1081 tgagcaccgg aaggaacgcc tggaacacgt cgtctgagac tccccgcccc gttcgaaggt
     1141 cgcgctggcc tgcgatcctg gtggccgcgc tcgcgatcag cgcctgccgc tcaccgaccg
     1201 agcccgtacc cgcgagcccg actttgacgg gtcagtgggt cggaaccgcc tcccgaggcc
     1261 cgtgcggcca gggctgggcc gaggactggt cgaccgtttc gatgacgctc gttcagacgt
     1321 ccacctccgt caccggcgag gtgttcgcct ccgaaggccg ccgcttcccc gtttcgagcc
     1381 tcgacggcat caccctgcat gtatccggcc tcgaaggctc ctccacctgc tccacctacg
     1441 cgttcttcat cagcgaccgg cagtacgacg gccacggaac gctcgtggcc ttctccggca
     1501 acctctctgg ccggtgctgc ggcaccctcg ccggcccctt ccgcgtccag cgtcgcccgc
     1561 agtaggacct tccgtccggg caccgcaccc acctgcattt ctgaagcgct cgcctcgacg
     1621 cgcggttcgg tgcccgactc ggcatgcacc ggactcgcgc ggcctctctg tccgcgccac
     1681 ggacgcccgc ggacagggct tcgcagggaa cccccgcctg ccacgtttct ccgcctcgtc
     1741 gctgccgcga acctcggcgc tcgccgcggg ggcgctccgg tgtccgggcc cgggtctcac
     1801 tcaaatggcg gcccggaccc ccagcgccca cacctcccgc ccatggggag accaccggcg
     1861 catccatgga gggccccgta cccgtccggc cgtcgccgca ccgatgccgc tcgacattcg
     1921 tgcgtcgctc cttctcctcc atgaacagcc acctgaccgt ttccgtgggt tggctgatac
     1981 ttcgaggcac ccggtctccc cgaagacccg ggggaacgac ctcctgagca gctacagcga
     2041 gatgacgaca ttcatcacgg agtcgccctg gaaccgtgcc cgaccgcgcg ttctcgtcgt
     2101 cgcctgctca gacgggcggc ttcaggagaa cctcgacgac ttcctgcagc ggtcccttcg
     2161 gatcacgcac tacgaccgcc tctacgctcc cggcggcgcc ggcgcattga cccgaagcgg
     2221 ctccgacttc attcgggccg ggttctatcg cagggagtgt ctcgacctcg tgcgcctcca
     2281 cggaatccag agcctgatcc tgctgtttca cggccccgcc atcgacggcc ccgccgaggc
     2341 cgtctgcgcc gactaccggc ggaagctccc ggcctcctcc ctccgagaca tccggcgcca
     2401 gcaggaggtt gacgctcgtg agcttcggtc ggccggcctg ggctggccga tgcccgtcga
     2461 gacgctcgtc ttccgatgcg aggtcggcgc cgatgacgtc gtgcgcttcg cagccctcgc
     2521 ggcagagccc ccacaaatcg cctgactccg gcgtcccgcg gactcggtcc gccgggctcc
     2581 cctcgccgcc gacgccggac ggtcgactcc gccgaatcgc gcgtagccgg gcatcggccg
     2641 acatctccgg ggcgacaaag atttcggcgg gtcgacgggt cgcgaatcgc ttctcggcct
     2701 tcgcgggccg cacgggacct ccaggccata ctcgtggttc aagcgaggag agagcgatgc
     2761 cggagacccc gaaggccaag gggccttctt cgcacttccc gttcatcgag aagacgtacg
     2821 gcaagccgat ctccctctgc atgggcgtcc tggagcgggc gcgccccgtc aagcacatgg
     2881 agctcgtgag cctgctcaag acggagcatg gactcggaca cggccacgca aacgccctgg
     2941 tcgcgcacgt tctcgcgacg gagtgacggc cggagggcgc ctccatgtcg gagccgggcc
     3001 gtccctggcg atggctcgct acggcgcgct gtcgacgcgt ggcgcccacg ttcgacgccc
     3061 gcggacatgg ctcccatcta cgctccggga atgtcaatcg ctacgactgc cgacacacct
     3121 cacgaccttc tcgccgtcct ccgccagcgc ctggtcctcc tgggacgctc cccccgcacc
     3181 gtcgacgcat atgccaattg ggtcggccgg ttctttcggt tccaccctcg ccgcgccccg
     3241 tccgagctcg gcatgggcga catcgagtcg ttcctcacgc acctggcgac cgtcgatcac
     3301 gtcgccgcca gcacgcaggg ccaggccctc gccgcgatcc tgtgcttctt ccgtgaggtc
     3361 cgctcgatcg acccgtccac gccgcttcag ttcggccgag cccggcacac gcgccacata
     3421 ccgacggtcc tcagccagcg cgaggctctc gctctcctcg gttgccttca aggcacgctt
     3481 cgcctcatct gccaggtgct gtacggctcc ggcctccgtc tctcggagtg cctgaatctc
     3541 cgcgtccaga acctggactt cgatcaccat acgatcgtcg tcatcaacgg caagggcgag
     3601 cgggacaggc tcaccgtcat tccgcccagc ctcgagaggc ctctcgaaac ccaccttcgg
     3661 cgcctctggg cgctgcatca ggaggacctg ctccgcggct acggccgcgt gcccctcccg
     3721 aacgccttcg cccgcaaggc tccgacggcc gcatgcaact tccgctggca gtacctgttt
     3781 cccggttccc gcctgatcga cgacccacgc accgggctcc gcggccgttg gcatctcgat
     3841 ccatccgtcg tccagcgggc ggttcgccaa gcggccctgc gcgccggcct ccggaagcgg
     3901 gtcggccccc acacgcttcg ccattcgttc gccacgcatc tcctggagcg cggctgcgac
     3961 ctccggcgca tccagtcctt gctcggccac gcccatctcg agacgacggc gatctacacg
     4021 cacatcacgg acctcgcctc cgcctccatt ccctcgcccc tcgagcccgc cgtcgtagtc
     4081 gcctcctcgt cgccgaaagc ctgaccccgc gtcccgcggc tcgcggcgcc cggcaccgcg
     4141 agccgctgag gccgaccgcc gggccgcggc agggccgcgc tccatccccg atccctcgga
     4201 cggggcggtg cggcggactc tcgcggaccg gcgcggcttg cgatcctcgt cgccgcccgc
     4261 atgtcgcgcg cggcagagtc cgctccgccc gagtccggag ccggccccgc cgttccgatc
     4321 aggctggtcg aagccttccc tcgttcgctc gccggcgagc tccgacggca tggcgggtcc
     4381 cgcccccgcc ggaccgatcg cccgtcgcgt gacggggatc cgtccggccc ggcgcgccgc
     4441 ggacttcgcc tcgttcgccg ctgacgctcg cgatcggcct ggcggatcct cggcgtcgtg
     4501 aggccgtcgc ccggcgcacg acggacgcag ggctgcgaac cgcttcccca ccgcggccgc
     4561 tcggaggacc ggatgaccgc aatcggacgt ctcggactgc tcctgggcct cgcgttcgcc
     4621 ccgctcgtgc acttcggacc acgcccctgg gcgcaggtcc tcgtgggcgc cctcgtcgtt
     4681 ctcgccctcc tcgcggcggc cgtcctcacc tggcgtcgcg ccggcctgcg tctcgcttcc
     4741 gcgggcatgg ccgtcgcggg cctcgcaatg gcgctgctca tgcgaatcct catccgcggg
     4801 cctgacccct tcgccgcgcc gccatcggcg ctcatcgcgt tcggcctcgt cctgctcctc
     4861 gccacggcct tcctgctcgc ccagtcccga accgccccgg agctctggga ccagtggcgc
     4921 cggcaccagc gcaccgtgac gctcgcggac gtgctctgct tcagacagat cccgagactc
     4981 cccggcgcgg gaccctcgag cccgccgatc cgggcctgag cctcgaaggg ggagtcgaaa
     5041 ggccgcccac gccgccgagc ttccaacccg gtcttcgccg gggcgctcgc gaacgccccg
     5101 gcccccgttc gggacggtag actccgagcc cggccccgca acgcagcggc cttcgccgcg
     5161 cgagatccga cggccgcggg cactcacgac cggccctcgg tgccgaagga gtgacgaatg
     5221 aagctaccgg ttcgcctggt ttcgctgctg gcctcctgca cgcccgcctg ggccgccgcc
     5281 gcggaggtcc tgccggaggc gccgcgatcg gccgggaacg tcggggcctc gggcctctgg
     5341 gtcctcgcga acatctgctt cgcgggcatg agggcgcaga acagcccgcg cgcgggatgg
     5401 cgcatcgtcg ccttcctctt cgggctcccc ggaaccctcg tgacgtactt cgtcgtggcc
     5461 gagggaagcg agcgcgccta cgggatcgac attcccagga agcgatgagc ccgggaagaa
     5521 cgcttcacga cgctcgcgtg ccgccgacgt cctcgatgag gcacgtcgag gagatccgat
     5581 gcgcacgact ctgaccgcac tcgtcctctg cggcctcggc ttcgcgggat gcgcgacccg
     5641 aggggtcgct ccccctctct cccccgaggt cgagctctcc ctggacgact tccggcgcgc
     5701 cctcgacgag gccacgccct ccgatcccgt cttcgatgac cgggaccctt ccgtgaaggc
     5761 gcgcctgact ccccctgtcg agctccaccg cgttcagccc gagctcagcc gcgcggcccg
     5821 cgcgcagtcc cgcgagggga acgtggcgct cgcgtgcgtc atcgagcgca acggctcggt
     5881 ctcgaacgta aaggtgctct ccgcgaccgg ccacggcgac ttcacggccg cctccgtcgc
     5941 ggctgttcgc cggtggcgct acaccccggc gaagatcgac ggctcgcccg tcaggctgct
     6001 tctcaccgtc accacgtcct accgcgtccg ctgaacgggc ctctctcttc cacgcatccg
     6061 gaggccacgc ccacgcggct cgcgccttcg accggggtgt cacgagccgg ctcggtcccg
     6121 gcggccggca gcggccggcg ctgccggacc gctagggtgc cggggtcgac tccgggggtg
     6181 atccggcccc cgccgccctc gagctcgcga ggatctcggt gtattcggcc atccggatgt
     6241 tcgactcgga gcgcgtctgg atgaggatgt ggttcttcgt ccccttctcg agcgccgaga
     6301 cggcgttgaa gaggcgggcc tgcgcgtctg agacccgggc ggcgaactcc tcccgggtgc
     6361 cggccggcgc aaggacgatg gtcacgagct ccgtcgtggc cttgcggacg gcgtcgatgt
     6421 cccccgcgcg tgggaaggac gagtcgacgg gaacgggctt tccgagctcg gtgagatagg
     6481 tgaccgcggc gagcccctcg tccgacgccc ggtaggcgtc cgccgcttcc caggacgggg
     6541 cggttccctc ctccgcggga gcctggccgg ccacggcctc gtcgtagcgc gaggcgaggt
     6601 ggctcgccgc ggcccgggcg acgtgggcgt cgacgagcgc cgccttcgtc gcgagctgct
     6661 cgggagtcgg tccgccgccg c
//
LOCUS       DAOONL010000162         6657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13696_length_6657_cov_19.888822, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000162 DAOONL010000000
VERSION     DAOONL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13696_length_6657_cov_19.888822"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PLP50_15490"
     CDS             <1..1200
                     /locus_tag="PLP50_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA52995.1"
                     /translation="NGAKQWISNGGYADVYTILANAPAGPTWFILEKGAEGFSHGKPE
                     DKHGLRISNTAALSLSDVYVPVENLVGGVEGQGLLQAQLVFGYTRLMVAAFGLGAGWA
                     ALDRAIPYSKDRLQGGAPLSEKQGYTHKLIVPHAVRLEAGRAYIEETAERLDAGEEML
                     NTEGAIAKYLATEAGLAAAEASIQALGGYGYTREYMVEKIRRDVRITTIYEGTSEIME
                     MTIARDRWQQHLKTRGQHYHDKAREMEALEAAQPGCGARFAALALHSLAEGLERFRAQ
                     RLTRHQHVLFRAGRMIALAEGAMVLAKRAARAAKGELSDKADRRLDAAALAAVSRANG
                     REAAFEVAQEALRWAVGADGVPASDLAAFTAAIRLPEISGAQAGLMTDLDLVSDALYG
                     RTPAPRA"
     gene            1215..>6657
                     /locus_tag="PLP50_15495"
     CDS             1215..>6657
                     /locus_tag="PLP50_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPA52996.1"
                     /translation="MEDTSARAVAVVGVGAIMPGAPSARAFWENITSGAYGISEVDPK
                     RWDPALYWDPDPKAPRKTYSKIGGWVREFPWEPVKWRLPIPPLVEAAMDDGQKWAIAL
                     TREVLADFGYPERPLDLDRTAVILGNAMGGEKHYQTAFHIFTPEVEAELLASPAFAAL
                     PADVKARTIDEMRRGVAGRLPDVTEDTMPGELSNCIAGRVANLFNFHGANYVTDAACA
                     SALAAMSAAVDGLVAGKFDAVLTGGLDRNMGPTSFVKFCKIGALSATGSRPYADGADG
                     FVMGEGAAFFLLKRLADAERDGDRIYAVIRGIGASSDGRGKGITAPNPVGQRFALRHG
                     WQVAGLDPATCSYVEGHGTSTKVGDVVEVESLIEAFGAAGLPAHSIPLGSVKSNIGHL
                     KAGAGAAGFFKAAMALAHKVVPPSLNFERPNPNLDWSKSPFYVNTELREWAKPACGVR
                     RAGLSAFGFGGTNFHVVLEEHVPGTLTKGSRPAQVSVPATPAAAPAAAPKSPLRGALL
                     VGAADEAELAKKLHAAQDEARQGRAPEPAPPSEAALRAPERVAIDFGNPHELAEKIEK
                     ALKALAGGKPAAWRPLRGQGIFRGHGPAPKVAFLFTGQGSQYVNMARTLRDLEPIVAE
                     TFAQADRRMEQILGRTISSYIFFDTKEPAAIAAAEEELKQTSITQPAVLTTDIALGRL
                     LNEYGIAPDMVMGHSLGEYGALVTAGVLPLEDALEAVAARGREMSRVSVADNGTLAAV
                     FAPVDVIEKTLEGIEGYVVVANYNSNGQSVIGGSTASVAAAVEAFKAAGITTAYIPVS
                     HAFHTKIVEPATVPFRTVLERLRIGGPKLPVVANVTGDLYPTDGDVRTQAIDLLSRQI
                     ASPVRFVRGLERLYEAGVRVFVEVGPKRAVQGFVEDVLGHRPDVHSLATNHPRLGDVV
                     SFNQAICGFWAAGIGTPRVEEKPRPVELAPAVRPAAPTLAVPAPTPAAAPAPVAASAP
                     LLSSGGSGDPIRDLGLLFSEFLEKGMKIHRGEGLAASATAAPAEEPVVITGSSLGLPG
                     RPRMFQDDNVAAILRGDQFIGRIPEKILRAMATKHITRLVKSEHGESRFEEVTSTDEV
                     IKLAGRAGELDLAEEFGVPAERIPALDVVTQLAIAAGLEALHDAGIPLVMRYKTTTKG
                     TKLPERWVLPEALQDDTGIVFASAFPGYDSFAERLRGYFLDHERRERLTDLEALRATL
                     AGKGASAEALAELDGRIADLKGLIEREHYTFDRRFLFQILSMGHSQFAELIGARGPNT
                     QVNAACSSTTQAVSVAEDWIRTGRARRVLVIAADDATSDQMMEWIGAGFVATGAAALD
                     EKVEDAALPFDRRRHGMVIGMGAIGLVVEGAEAARERGITPACRLLSAVTANSAFHGT
                     RLDVNHISQVMETLVSQAERKWGVDRHAIAPKMIFVSHETYTPARGGSASAEIFALRG
                     TFGASVDSIVMANTKGFTGHPMAVGIEDALAVKMLETGIVPPIPNFREVDPELGPLHL
                     SKGGRYDRDYTLRLAAGFGSQIAMTLLERVPPPDGRRRAPEELGYAYRIHDAARYRAW
                     LSAISGYDAPDTEVEKRTFRVKNTGAPARASRRLDSPAAPATSRTAAPAPAPAPVPVP
                     AAPVVAPSAPAPAVVPARAPVAAAAAIDPVAERVLAIVAEKTGYAQEMLALDLDLEAD
                     LGIDTVKQAELFSAVREEWGIPRDDSRKLRDYPTLKHVIQFVRDGRPDLPPPGAGVPA
                     PAAAPAPAPAAAPAVVAPTPAAPAVDQVLTRVLAIVTEKTGYPVEMLDPELDLEADLG
                     VDTVKQAELFSAVREEYGIPRDDSRKLRDY"
BASE COUNT          958 a         2574 c         2267 g          858 t
ORIGIN      
        1 aacggcgcca agcagtggat cagcaacggc ggctacgccg acgtctacac gatcctcgcg
       61 aacgcgccgg cgggacccac ctggttcatc ctcgagaagg gggccgaggg cttctcgcac
      121 ggcaagcccg aggacaagca cggcctgagg atctcgaaca cggcggccct ctcgctgtcg
      181 gacgtctacg tccccgtcga gaacctcgtc ggcggcgtgg aggggcaggg gctcctccag
      241 gcgcagctcg tcttcggcta cacccgcctc atggtcgccg cgttcggcct cggcgccggc
      301 tgggccgcgc tcgaccgggc gatcccctac tcgaaggacc gcctccaggg cggggcgccc
      361 ctctccgaga agcagggcta cacgcacaag ctcatcgtcc cgcacgccgt gcggctcgag
      421 gcgggccgcg cctacatcga ggagacagcc gagcggctcg acgcgggcga ggagatgctg
      481 aacaccgagg gcgccatcgc gaagtacctc gcgaccgagg cgggcctcgc cgcggccgag
      541 gcctcgatcc aggcgctcgg cggctacggc tacacccgcg agtacatggt cgagaagatc
      601 cgccgcgacg tccgcatcac gacgatctac gaggggacct cggagatcat ggagatgacg
      661 atcgcgcgcg accgctggca gcagcacctg aagacgcgcg gccagcacta ccacgacaag
      721 gcccgcgaga tggaggcgct cgaggccgcg cagcccggct gcggcgcgcg cttcgccgcc
      781 ctcgcgctcc actccctcgc cgaggggctc gagcgcttcc gcgcccagcg cctcacgcgc
      841 caccagcacg tcctcttccg cgccggccgg atgatcgccc tcgccgaggg ggcgatggtc
      901 ctcgcgaagc gcgccgcccg cgccgcgaag ggggagctct ccgacaaggc cgaccggcgg
      961 ctcgacgcgg ccgcgctcgc ggccgtctcc cgcgcgaacg gccgtgaggc cgccttcgag
     1021 gtcgcgcagg aggcgctccg ctgggcggtc ggcgccgacg gcgtccccgc ctccgacctc
     1081 gccgcgttca ccgcggcgat tcgcctcccc gagatttccg gggcgcaggc cggcctgatg
     1141 accgacctcg acctcgtctc cgacgccctt tacgggcgga cgcccgctcc gcgggcctga
     1201 gaggaagcca cctcatggaa gacacgtctg cccgcgcggt cgccgtcgtc ggcgtcggcg
     1261 cgatcatgcc cggcgccccg agcgcccggg ccttctggga gaacatcacg tcgggggcct
     1321 acgggatctc cgaggtcgac ccgaagcgct gggacccggc gctctactgg gaccccgatc
     1381 cgaaggcgcc gcggaagacc tactcgaaga tcggcggctg ggtccgcgag ttcccctggg
     1441 agcccgtgaa gtggcggctc ccgatcccgc cgctcgtcga ggcggcgatg gacgacgggc
     1501 agaagtgggc gatcgcgctc acgcgcgagg tcctcgccga cttcggatac ccggagcgcc
     1561 ctctcgacct cgaccggacg gccgtgatcc tcgggaacgc gatggggggc gagaagcact
     1621 accagaccgc cttccacatc ttcacgcccg aggtcgaggc ggagcttctc gcctcgcccg
     1681 cgttcgcggc gctcccggcc gacgtgaagg cccggacgat cgacgagatg cgccggggcg
     1741 tcgccggccg cctccccgac gtcaccgagg acacgatgcc gggcgagctt agcaactgca
     1801 tcgccgggcg cgtcgcgaac ctcttcaact tccacggggc gaactacgtc accgatgccg
     1861 cctgcgcctc cgccctcgcg gcgatgagcg ccgcggtcga cggcctcgtg gccgggaagt
     1921 tcgacgcggt cctcacgggc ggcctcgacc ggaacatggg gccgacgagc ttcgtcaagt
     1981 tctgcaagat cggcgcgctc tcggccaccg gctcgcgccc ctacgcggac ggggccgacg
     2041 gcttcgtcat gggggagggg gcggccttct tcctcctcaa gcgcctcgcc gacgccgagc
     2101 gcgacggcga ccggatctac gccgtcatcc gcggcatcgg cgcctcgagc gacgggcgcg
     2161 gcaaggggat caccgccccg aacccggtcg gccagcgctt cgcgctccgg cacggctggc
     2221 aggtcgcggg gctcgacccg gccacctgct cgtacgtcga ggggcacggc acctcgacga
     2281 aggtcggcga cgtcgtcgag gtcgagagcc tcatcgaggc gttcggcgcg gcgggtctcc
     2341 cggcgcactc catcccgctc ggttcggtca agtcgaacat cgggcacctg aaggcgggcg
     2401 cgggcgccgc cggcttcttc aaggcggcga tggccctcgc gcacaaggtc gtcccgccga
     2461 gcctcaactt cgagcgcccg aacccgaacc tcgactggtc gaagtcgccg ttctacgtga
     2521 acaccgagct gcgcgagtgg gcgaagcccg cctgcggagt ccgccgcgcg ggcctgtcgg
     2581 ccttcggctt cggcggcacg aacttccacg tcgtcctcga ggagcacgtc ccgggcaccc
     2641 tcacgaaggg gagccgcccg gcgcaggtct ccgttcccgc gacgccggct gcagctcccg
     2701 ctgccgcccc gaagtcgccc ctccgcggcg cgctcctcgt cggcgccgcc gacgaggcgg
     2761 agctcgcgaa gaagctccac gccgcgcagg acgaggcgcg gcagggacgg gcgcccgagc
     2821 cggccccgcc gagcgaggcc gccctccgcg ctcccgagcg cgtcgcgatc gacttcggca
     2881 acccgcacga gctcgccgag aagatcgaga aggcgctgaa ggcgctcgcg ggcggcaagc
     2941 ccgccgcctg gaggccgctc cgcgggcagg ggatcttccg cggccacggc ccggcgccga
     3001 aggtcgcctt cctcttcacg ggccagggct cgcagtacgt gaacatggcg cgcaccctgc
     3061 gcgacctcga gccgatcgtc gcggagacgt tcgcgcaggc cgaccggagg atggagcaga
     3121 tcctcggccg gacgatcagc tcgtacatct tcttcgacac gaaggagccc gcggcgatcg
     3181 cggcggccga ggaggagctg aagcagacct ccatcacgca gcccgcggtc ctgacgaccg
     3241 acatcgccct cggccgcctc ctcaacgagt acggcatcgc ccccgacatg gtcatggggc
     3301 actccctcgg cgagtacggc gccctcgtca cggctggcgt cctcccgctc gaggacgccc
     3361 tcgaggcggt cgcggcgcgc ggccgcgaga tgtcgcgcgt ctccgtcgcc gacaacggca
     3421 cgctcgccgc cgtctttgcc ccggtggacg tcatcgagaa gacgctcgag gggatcgagg
     3481 gatacgtcgt cgtcgcgaac tacaacagca acgggcagtc cgtcatcggc ggctcgaccg
     3541 cctcggtcgc agcggccgtc gaggcgttca aggcggccgg catcacgacg gcctacatcc
     3601 cggtcagcca cgccttccac acgaagatcg tcgagccggc gaccgtcccg ttccggacgg
     3661 tcctcgagcg gctccggatc gggggaccga agctccccgt cgtcgcgaac gtcacgggcg
     3721 acctctaccc gacggacggc gacgttcgca ctcaggcgat cgacctcctc tcccgccaga
     3781 tcgcctctcc cgtccgcttc gtgagggggc tcgagcggct ctacgaggcg ggcgtccgcg
     3841 tcttcgtcga ggtcggcccg aagcgggccg tccagggctt cgtcgaggac gtcctcggcc
     3901 accgccccga cgtccattcc ctcgccacga accacccgcg cctcggcgac gtcgtttcct
     3961 tcaaccaggc gatctgcggc ttctgggccg ccggcatcgg cacgccgcgc gtggaggaga
     4021 agccgcgacc cgtcgagctc gccccggccg tgcgtcccgc cgcgccgacc ctcgcggtcc
     4081 cggcgccgac gcccgcggcc gctccggccc cggtcgcggc ctctgcgcct ctcctgagct
     4141 ccggcggctc gggcgacccg atccgcgacc tcggcctcct cttttccgag ttcctcgaga
     4201 aggggatgaa gattcaccgc ggcgagggcc tcgcggcgtc cgcgacggct gcgccggcgg
     4261 aagagcccgt cgtcatcacc ggctcctcgc tcgggctgcc gggccggccg cggatgttcc
     4321 aggacgacaa cgtcgccgcg atcctccgcg gcgaccagtt catcggccgg atccccgaga
     4381 agatcctccg ggcgatggcg acaaagcaca tcacccggct cgtcaagagc gagcacggcg
     4441 agagccgctt cgaggaggtc acctcgacgg acgaggtgat caagctcgcg ggccgagccg
     4501 gagagctcga cctcgccgag gagttcggcg tcccggccga gcggatcccg gctctcgacg
     4561 tcgtcacgca gctcgcgatc gccgccggcc tcgaggcgct ccacgacgcg gggatcccgc
     4621 tcgtcatgcg ctacaagacg acgacgaagg ggacgaagct ccccgagcgc tgggtcctcc
     4681 ccgaagcgct ccaggacgac acgggcatcg tcttcgcgtc cgccttcccg ggctacgact
     4741 ccttcgccga gcggctccgc ggctacttcc tcgaccacga gcggcgcgag cggctcaccg
     4801 atctcgaggc gctccgcgcg accctggccg gcaagggcgc gtcggccgaa gccctcgccg
     4861 agctggacgg gcggatcgcc gacctgaagg ggctcatcga gcgggagcac tacaccttcg
     4921 accggcgctt cctcttccag atcctctcga tgggccactc gcagttcgcc gagctgatcg
     4981 gcgcgcgcgg cccgaacacg caggtcaacg ccgcctgctc ctcgacgacg caggcggtct
     5041 cggtcgccga ggactggatt cgcacgggcc gcgcccgccg cgtcctcgtc atcgccgccg
     5101 acgacgcgac ctccgaccag atgatggagt ggatcggcgc cggcttcgtg gcgacggggg
     5161 ccgcggcgct cgacgagaag gtcgaggacg ccgccctccc gttcgaccgc cgccgccacg
     5221 ggatggtcat cggcatgggg gcgatcggcc tcgtcgtcga aggggccgaa gcggcgcgcg
     5281 agcgcggaat cacccccgcc tgccgcctcc tctcggccgt cacggcgaac agcgccttcc
     5341 acggcacccg cctcgacgta aaccacatct cgcaggtcat ggagacgctc gtctcgcagg
     5401 ccgagcggaa gtggggcgtc gaccggcacg ccatcgcccc gaagatgatc ttcgtctccc
     5461 acgagaccta cacgccggcc cgcggcggca gcgcctcggc cgagatcttc gccctccgtg
     5521 ggacgttcgg cgcgtccgtc gactccatcg tcatggcgaa cacgaagggc ttcacgggcc
     5581 acccgatggc ggtcggcatc gaggacgccc tcgcggtcaa gatgctcgag acggggatcg
     5641 tcccgccgat cccgaacttc cgggaggtcg accccgagct cgggccgctc cacctgtcga
     5701 aggggggacg ctacgaccgc gactacacgc tgcggctcgc cgcgggcttc ggctcgcaga
     5761 tcgcgatgac gctcctcgag cgcgtcccgc cgcccgacgg ccgccggcgc gctccggagg
     5821 agctcggcta cgcctaccgg atccacgacg ccgcgcgcta ccgcgcctgg ctctccgcga
     5881 tcagcggcta cgacgcgccc gacacggagg tcgagaagcg aacgttccgc gtgaagaaca
     5941 ctggagcccc ggcccgcgcc tcccggcggc tcgattcgcc ggccgccccc gcgacttcgc
     6001 gaacggccgc tcccgctccg gccccggcgc ctgtcccggt acctgccgcc ccggtcgtgg
     6061 ccccgtccgc cccggctccg gccgtggtgc ccgcccgcgc tccggtcgcc gccgcggccg
     6121 cgatcgatcc cgtcgccgag cgcgtcctcg cgatcgtggc cgagaagacc ggctacgcgc
     6181 aggagatgct cgcactcgac ctcgacctgg aggccgatct cgggatcgac accgtcaagc
     6241 aggccgagct cttcagcgcc gtgcgcgagg agtggggaat cccgcgcgac gacagccgca
     6301 agctgcgcga ctacccgacg ctcaagcacg tcatccagtt cgtccgggac ggacgcccgg
     6361 acctgccgcc gcccggcgcc ggggttccgg cgcccgccgc ggcgcctgcg ccggctccgg
     6421 ccgccgcgcc cgcggtcgtc gctcccacac cggccgctcc cgcggtcgac caggtcctga
     6481 cgcgcgtcct cgcgatcgtg accgagaaga cgggctaccc cgtcgagatg ctcgacccgg
     6541 agctcgacct cgaggcggac ctcggcgtcg acacggtcaa gcaggccgag ctcttcagcg
     6601 cggtgcgcga ggagtacggc atcccgcgcg acgacagccg caagctccgc gactacc
//
LOCUS       DAOONL010000163         6651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13717_length_6651_cov_12.286386, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000163 DAOONL010000000
VERSION     DAOONL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13717_length_6651_cov_12.286386"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLP50_15500"
     CDS             <1..513
                     /locus_tag="PLP50_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA52997.1"
                     /translation="SGKVPFQLEPVRLDILAGEVVESFSERANALGVRLEMAPAPAVV
                     IPGDERWLRQLVLNLIDNAVRFSTGMDAHTAEGQVRVTVWTEDPLAFLAVDDDGPGIP
                     EPNLDRVFERFFRSDTARTRSVEGGAGLGLAICAWIVREHGGTIRALQRSGGGTRMIL
                     AVPRSSSQPR"
     gene            911..1102
                     /locus_tag="PLP50_15505"
     CDS             911..1102
                     /locus_tag="PLP50_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52998.1"
                     /translation="MHRKMYADYKMTVAIHPKAPENPWLRKMRDHCDRYIKDAEALAV
                     DAEKFAEYHGFRAAELQGK"
     gene            1171..1401
                     /locus_tag="PLP50_15510"
     CDS             1171..1401
                     /locus_tag="PLP50_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA52999.1"
                     /translation="MHTLTVSIQGAANDSTSVELPDDQALPVYVKLKEVLAGTRAARR
                     AIRKDSGSAAADGQNGDAGRNGVGDSSADLDR"
     gene            1409..1825
                     /locus_tag="PLP50_15515"
     CDS             1409..1825
                     /locus_tag="PLP50_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53000.1"
                     /translation="MISFLIAAACVVFVLSLPVSATKAGQTLRRWAGVLFLLALAPAL
                     FVGLVGAMGGSSGTPQAVTGAGGGVDALGTVLGFLILSPLAYGILQLRKRFNPKSRDP
                     WADYVDRKAAGKKVVDPKAAKPVLPGGTFGPFGGSP"
     gene            1822..>2870
                     /locus_tag="PLP50_15520"
     CDS             1822..>2870
                     /locus_tag="PLP50_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53001.1"
                     /translation="MSLLDFVGSILHRERRRAYEMAVAAELPKLAPPKPSSEVMEEVR
                     GRLAGPGALTFGRLPSGQIVRVKGDTSMKSALVIGAPGSGKTRFLLGLLIELISRALR
                     DEVEIELVDPKTETYSLTCQILAALYLGGDFALREAIRRRVRVIDWSREAVSPVTPFD
                     NTDGIVSNAYLAHLRTDATVQASPQTYTESLKQALFMLSWLLTEKGFPPNLRFVSKLY
                     SDPAFRAKVIADVAEPDLRNYFAELERHMARPTADALLRRFQHDLSFPEVRLSIGIPP
                     ASLRNILPAGSPRITLGNYGSGMALPASVGKERAAHRVTDVLLRAPRRNPKRPGLLFL
                     EEAPTLLAGGSELVEP"
     assembly_gap    2871..2970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2971..3228
                     /locus_tag="PLP50_15525"
     CDS             <2971..3228
                     /locus_tag="PLP50_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53002.1"
                     /translation="VNDPSDMAKRSEAELLEVFRREIASVSMVPAKTAADLIARFEAE
                     VVDRATIPPAPPKKAAPKGAAPVRGIADLLRMMGGSPGTGA"
     gene            3231..3536
                     /locus_tag="PLP50_15530"
     CDS             3231..3536
                     /locus_tag="PLP50_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53003.1"
                     /translation="MGPTIRTAFRLSALATLYLLAAPVYLIRAIGEWHRIARGLAIVR
                     RGTIACPHCSFVNPINVLSTCHRCGVTEFGSRLYCSNCRQVTRWFDCARCHATIKVF"
     gene            3610..3900
                     /locus_tag="PLP50_15535"
     CDS             3610..3900
                     /locus_tag="PLP50_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53004.1"
                     /translation="MPQHHFGIVVPAVQPPTGVLNLHDADWENLRPIVREHLDGRIEI
                     TTHTGLRVLLFGSVVEIPVYLVRVWSEHPIEDIATVVQPELVRIANTHTENV"
     gene            4055..4672
                     /locus_tag="PLP50_15540"
     CDS             4055..4672
                     /locus_tag="PLP50_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53005.1"
                     /translation="MTVAPSELDLGRTPEPCALPIPTSLSTPPALWPAGTPPTAAVRL
                     ERETSVPDGIGRTAVHGWRGTRHLTSTETHLSRKEALGPTTRLSLAAWYPLPHTPMRD
                     TTRLELLVPLADNDTVPFSDAAFAALEDFLVQLAGGFTRRGDVEGAWKAPDGRVMRDR
                     SRSYAVTVPEHLTDRVASSIDHYVRRQFRQQATFVETLPARAVAF"
     gene            4677..5603
                     /locus_tag="PLP50_15545"
     CDS             4677..5603
                     /locus_tag="PLP50_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53006.1"
                     /translation="MVPSDRAGADPGPTPHEPPDRGDSARSDLVILSGATGSSGRPRS
                     AAIGPLDPRRPPETLTDRDVSALAFIGRSQEAAQYQLHEAVFPGLGEAVVSRRIRKLA
                     ARGLLAIERWNKVGINRLRLRPAGRDAIVERKGARDDELFLLRAPLAQSAVAHHLWVV
                     DCRLIIERLPAPPTSAQPSWVLERRWNPRPAAIPDVLAIWRPTRESPGHLLALEIDIG
                     TEAIRRTFVPRLVALAETLVPFAGTSPAFISVLTRGSRRVTAIQQAVSSASIALPVVV
                     DSLPPAAGRPAFAALRILLTVGAKSPSHPGPA"
     gene            5837..6622
                     /locus_tag="PLP50_15550"
     CDS             5837..6622
                     /locus_tag="PLP50_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53007.1"
                     /translation="MTLCPSCQSIFTGTLPPTCPTCGAALGTSIEANRRQGSLLVPKM
                     PSIRPAARPKLPVEVTLGIEIDRTGSSAAFQAGISFSSEVLLETIAAKVKSLTVYVGT
                     HGDEECGEAPVLHTAGGTREQAMADIRGITFGGGGDAPETHLDGIRNFLETVPWPMDK
                     SRTRGAFVAFLTDDTKPDRTGATATQIGKAIRDKDLLFYAVCQKTKELEALVSAAGGL
                     LMPISNTPDRADLENAAAQVSASLIATIGAGGTLPMTMPGGAR"
BASE COUNT         1025 a         2476 c         2048 g         1002 t
ORIGIN      
        1 agcggcaagg ttcccttcca gctcgagccg gttcgtctcg acatcctcgc cggcgaggtg
       61 gtggagtcgt tctcagagcg cgcgaacgct ctcggagtcc ggctcgaaat ggcgccagca
      121 cccgccgtcg tcatcccggg cgatgagcgc tggcttcgcc agctcgtcct caacctcatc
      181 gacaacgcag tccgattctc gactggaatg gacgcgcaca ccgccgaggg ccaagtccgt
      241 gtcacggtgt ggaccgagga cccgctcgcg tttctcgcag tagacgacga cggacccggg
      301 atccccgagc ctaatctgga tcgcgtcttc gagaggttct ttcgtagcga caccgcccgc
      361 acgcgaagtg tcgagggcgg cgccggcctt ggcctggcaa tctgcgcctg gatcgtgcgc
      421 gagcacggcg ggaccattcg cgccctccaa cgctccggag gcggcacgcg catgattctg
      481 gcggtgccaa ggtcctcaag tcagccgcgg tagagtacgt gccgttcctc taaggcggct
      541 ctaatctcgc ccgcgcaaat tccctccgaa gacaccgggc ctgacggaag ccggtcgccc
      601 gggatcgtcc ggcgactcgc aacccgcctt ccgatgttca cccgtcctcg gacgctcggg
      661 gtccggagac attcgcgcgg aggtactcgc acatgacacc catgaacgga cacgacactc
      721 cccgactcgg tcgcgccgcc gtcctcgccc tcggcgtagc gttctccgcc tccggcgcct
      781 tcgcgttgcc gcagtcggtc gcgccccagt ctgagagcgc gcaggcacca aaggttgccg
      841 tcacggccga agaccacctc gcgaaagcca ccgagtatcg gaagaaggcg cataccgcga
      901 ggaggccgcc atgcacagaa agatgtacgc cgactacaag atgaccgtgg ccatccatcc
      961 gaaggcgccc gagaatccgt ggctccgcaa gatgcgtgac cactgcgacc gatacatcaa
     1021 ggacgccgag gccttggccg tggacgcgga gaagttcgcc gagtatcacg gcttccgtgc
     1081 cgctgagctc caggggaaat gagcagttcg acgctgtccc ggtaaccacg gtcgtaaacc
     1141 ctaccgtcct cccatcgaaa ggaggacacc atgcacaccc tcaccgtctc gatccagggc
     1201 gccgcgaacg actcgacgag cgtcgagctc ccggacgacc aggctctgcc cgtctacgtg
     1261 aagttgaagg aggttcttgc cgggacccgg gcggcccggc gggcgatccg gaaggactcc
     1321 ggctccgccg cggctgacgg ccagaacggc gacgccggga ggaacggagt cggcgacagt
     1381 tcggctgacc tcgaccgctg aggaaccggt gatctcgttc ctcatcgccg cggcctgcgt
     1441 cgtcttcgtc ctctcgctgc cggtctcggc gacgaaggcc gggcagacgc ttcggaggtg
     1501 ggccggagtg ctcttcctcc tggcgctggc gccggcactc ttcgtggggc tggtcggcgc
     1561 catgggcggc tcgagtggca cgccccaagc cgtgaccggc gccggtggcg gcgtcgacgc
     1621 gctcgggacg gtcctcgggt tcctgatcct ctctcccctc gcctacggga tcctccagct
     1681 ccgaaagcgc ttcaacccga agagccgcga cccgtgggcg gactacgtcg accggaaggc
     1741 ggccgggaag aaggtcgtgg acccgaaggc ggccaaacca gtcctgccag gcgggacgtt
     1801 cggcccgttc ggaggctctc cgtgagcctt ctcgacttcg tcgggtcgat cctccaccgg
     1861 gaacggaggc gcgcctacga aatggccgtc gccgccgagc tgccgaagct cgcgccgccg
     1921 aagccttcgt ccgaggtgat ggaggaggtc cgcggccggc tcgccgggcc tggcgcgctc
     1981 acctttggca ggctgccgtc cggccagatc gtccgtgtga agggcgacac gtcgatgaag
     2041 agcgccctcg tgatcggggc gcccgggtcg gggaagaccc ggttcctcct cggcctcctg
     2101 atcgagctga tctcccgggc ccttcgcgac gaggtggaga tcgagctcgt cgatccgaaa
     2161 acggagacct acagcctcac ctgccagatc ctcgccgccc tctacctcgg tggcgacttc
     2221 gctctcaggg aggcgatacg gcggcgcgtc cgcgtcatcg actggtcacg cgaagccgtc
     2281 tcccccgtca cgcccttcga caacacagac ggcatcgtct cgaacgccta cctcgcccac
     2341 ctccggaccg acgccacggt ccaggccagc ccgcagacct acacggagtc gctgaagcag
     2401 gccctcttca tgctctcgtg gctcctcacg gagaaaggct ttccgccgaa cctccggttc
     2461 gtctcgaagc tctactccga cccggcgttc cgggcgaagg tcatcgccga cgtcgccgag
     2521 ccagacctcc gcaactactt cgccgagctc gagcgccaca tggcgcggcc cacggccgac
     2581 gcactcctcc gccggttcca gcacgacctg tcgttccccg aggtccggct ctcgatcggc
     2641 atcccgcccg cgtcgctccg gaacatcctc ccggccggct ccccgcgcat caccctcggg
     2701 aactacggct ccgggatggc gctccccgcc tccgtcggga aggaacgcgc cgcccaccgg
     2761 gtgaccgacg tcctcctccg cgcgccgcgc cggaacccga agcgcccggg cctcctcttc
     2821 ctcgaggaag cgccgacgct cctggccggc gggagcgagc tcgtcgagcc nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaacgacc cctcggacat ggcgaagcga
     3001 tccgaggccg agctcctcga ggtcttccgc cgcgagatcg cctccgtctc gatggtcccg
     3061 gccaagacgg cggccgacct catcgcccgc ttcgaggccg aggtcgtcga ccgcgccacc
     3121 atcccgccgg ctccaccgaa gaaggctgcc cccaagggtg cggcgcccgt ccgcggaatc
     3181 gcggacctcc ttcggatgat gggtggctca ccggggacgg gggcatagac gtgggcccga
     3241 cgatccgcac cgcgtttcgg ctgtcggcgc tggcgacgct ctacctcctc gccgcgccgg
     3301 tctacctgat ccgcgccatc ggcgagtggc accggatcgc ccgcggcctc gccatcgtcc
     3361 ggcgcgggac gatcgcctgt ccccactgct ccttcgtcaa tccgatcaac gtgctctcga
     3421 cctgccaccg ctgcggcgtc acggagttcg ggagccgcct ctactgctcg aactgccggc
     3481 aggtgacgcg ctggttcgac tgcgcgcgct gccacgcgac catcaaggtc ttctgatcgt
     3541 gacgacctcc tcattcgctc gtgccaggaa ggaaggtgcc ccccgccaca ccacctacac
     3601 ctcacctcta tgccccagca ccacttcgga atcgtcgtcc ccgccgtcca accacctaca
     3661 ggcgtcctca atcttcacga cgccgactgg gagaacctcc gccccatcgt gcgcgagcac
     3721 ctcgacgggc gaatcgaaat cacgacgcac accggtctcc gcgtcctcct cttcgggagc
     3781 gtcgtcgaga tcccggtcta cctcgtgcgc gtctggtccg agcacccgat cgaggacatc
     3841 gccaccgtcg ttcagccgga gctcgtccgc atcgcgaaca cccacaccga gaacgtctga
     3901 cggtaccaac cggacggccc cttccccctg gacgtcgcca ccggccccgc cgggacggga
     3961 cgctgcacga aagacctgcc gggccccacg cgcgaaccgc gacgtggccc ccgcagcacc
     4021 tccggagtgc ggccccgtcc cggcggggca cggagtgacc gtcgcccctt cagaactcga
     4081 tcttgggcgg acgcccgagc catgtgcgct gccgataccg acgtcgctct cgaccccgcc
     4141 agccctctgg cctgcaggaa cgcccccgac cgccgccgtt cgtctcgagc gagagacgtc
     4201 cgtcccggac gggatcggca ggacggcagt ccacggctgg cggggcacga ggcatctcac
     4261 ttccaccgaa acacatctct cccggaagga agccctcggc ccgacaacgc gactctcgct
     4321 ggctgcctgg tatccccttc cccacacccc aatgcgcgac acgacccgac tcgagctcct
     4381 cgttccgctc gcggacaacg acaccgtccc gttctccgac gcggccttcg ccgccctcga
     4441 ggacttcctc gtccagcttg ccggcggctt cacccgccgc ggcgacgtcg agggagcctg
     4501 gaaggcgccc gacggtcgcg tgatgcgaga ccggtcacgg tcgtacgctg tgaccgtccc
     4561 cgagcacctc acggatcgcg tggcgtcgtc tatcgatcac tacgtgcgtc gccagttcag
     4621 gcagcaggcg acgttcgtcg agactctccc ggcgcgagcc gtcgcgttct aggaccatgg
     4681 tgccttccga ccgggcgggc gccgacccgg ggccgacccc gcacgaaccg ccggaccgag
     4741 gagacagcgc tcgaagcgac ctcgtcatcc tctccggagc aacgggaagt tcggggcggc
     4801 cccggtccgc ggcgatcggg ccgctcgacc cgcgacgtcc tcccgagacg ctcaccgatc
     4861 gcgacgtctc cgcactcgcg ttcatcggcc ggtcgcagga ggccgcgcag taccagctcc
     4921 acgaagcggt ctttcccgga ctcggcgagg ccgtcgtctc gcgtcggatt cggaagctcg
     4981 ctgcccgggg cctcctcgcc atcgagcggt ggaacaaggt cggcatcaac cggctgcgcc
     5041 tccggccggc cggccgcgat gccatcgtcg agaggaaggg tgcccgcgac gacgagctct
     5101 tcctccttcg cgccccgctc gcccagtcgg ctgtcgctca ccacctctgg gtcgtcgact
     5161 gtcgcctcat catcgagagg ctcccggccc ctccgacctc ggctcaaccg tcctgggtcc
     5221 tcgagcgtcg gtggaacccg cggccggccg caattccgga cgtcctagcc atctggcggc
     5281 ccacgcgtga gagccccggt catctcctgg ccctcgaaat cgacatcggg acggaggcga
     5341 tccggcggac gttcgtcccg aggctcgtcg ccctcgccga gacgctcgtc cccttcgccg
     5401 ggacgtctcc ggccttcatc tccgtcctga cccggggctc caggcgtgtc acggccatcc
     5461 agcaggctgt gtcctccgcc tcgatcgccc ttccggtcgt cgtcgactcc ctgcccccag
     5521 cggccggccg gccggcattc gccgccctcc ggatcctgct cacggtgggt gcaaagtcgc
     5581 cttcgcaccc cggtccggcc taagcccgca ccccacaggc atttcacacc tttcagcttc
     5641 gcggcgctta gggggcgcga gcgagtgagt gagcggcgag agggcgctgg gcgctgcctc
     5701 ggctcttcag cacgccgaag tgagtcagtt cgacggagtg cagggcagga gagtcgcttc
     5761 gggggaaggt cgcaggcgca ccggaccaca cctgaaggag agacacatga ccacctttgc
     5821 tccctcaaag aaacccatga cgctctgccc gtcgtgccag tccattttca cggggacgct
     5881 cccgccgact tgccccacct gcggggccgc gctcgggacg tcgatcgaag ccaaccggag
     5941 acagggctcc ctcctcgtcc cgaagatgcc gtccattcgc ccggccgccc ggccgaagct
     6001 cccggtcgag gtgacgctcg gaatcgagat cgaccgcacc gggtcgtcgg ccgccttcca
     6061 ggccggaatt tccttttcgt ccgaggtgct cctcgagacc atcgcggcga aggtgaagag
     6121 cctcacggtc tacgtcggca ctcacggcga cgaggagtgc ggcgaggcgc cggtcctcca
     6181 caccgccggc gggacgcgcg agcaggcgat ggccgacatc cgcggcatca ccttcggcgg
     6241 tggtggcgac gcgcccgaga cccacctcga cggcatccgc aacttcctcg agacggttcc
     6301 ctggcccatg gacaagagcc ggacgcgcgg cgccttcgtc gcgttcctca cagacgacac
     6361 gaagcccgac cggaccgggg cgaccgcgac gcagatcggg aaggcgatcc gcgacaagga
     6421 cctgctcttc tacgccgtct gccagaagac gaaggagctc gaggccctcg tgagcgcggc
     6481 aggtggactc ctcatgccga tctcgaacac gccggatcgc gccgatctgg agaatgcggc
     6541 cgcccaggtg agcgcatctc tcatcgcgac catcggcgcc ggcgggacgc tcccgatgac
     6601 gatgccgggc ggtgcgcggt gaagagcgtc atccccaccc tccagcgcgt c
//
LOCUS       DAOONL010000164         6632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13772_length_6632_cov_23.152045, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000164 DAOONL010000000
VERSION     DAOONL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13772_length_6632_cov_23.152045"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLP50_15555"
     CDS             <1..1049
                     /locus_tag="PLP50_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPA53008.1"
                     /translation="WVARTGSRELFTLCVLATAIGVAYGSAKLFDVSFALGAFFAGMM
                     MRESEFAHRAADESLPLRDAFSVLFFVSVGMLFDPRVLVEQPVKVLVVVAIIMLGKTL
                     GAVGLVLTMRYPLHTALTVGAALAQIGEFAFIIAVLGRDLGLLPAAGHSLILVGSIIS
                     IGLNPVVFSTVGPIAEWLKRHSALARRLAVLEHETAALPTTTDEALLTGQVVLVGYGR
                     VGRRIAEALTERRIPFVVAEMSREAVAALREKEIPAVSGDASDPAVLVQAHVATAAML
                     VIATPDPVKVRKMVEVARTLNPSIEIVLRTHTEEEAELLREEQAGKVFMGEHELALAM
                     AGHVLARMDRPSAA"
     gene            1170..2156
                     /gene="hflK"
                     /locus_tag="PLP50_15560"
     CDS             1170..2156
                     /gene="hflK"
                     /locus_tag="PLP50_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPA53009.1"
                     /translation="MEIQFPDGTRLRTPSIPVRLVTRAALGVLAFVVLFSAWFTIEPE
                     EAGLVLRFGKYARTVSPGLHLKLPSPIERVVKVPVERQLKEEFGFRTEASGVSRSTYS
                     QEDLTSESLMLTGDLNVAVVEWTAQYRVRDPYKFLFKVRSVQKTFRDMNETVMREIIG
                     DRSVNEVLTVGRQEIAAEVEQRLQDLCDQYETGIKVEQIVLQDVNPPDPVKPSFNEVN
                     QAQQEREKLINQARADYNQIIPRASGQAQQTIEQAEGFATDRVNRARGDAELFVKVHA
                     AYQRAPEVTRRRMYLETMGEIYPKVKRKVVLDEKLKGVLPLLPLAGAPEVKP"
     gene            2153..3091
                     /gene="hflC"
                     /locus_tag="PLP50_15565"
     CDS             2153..3091
                     /gene="hflC"
                     /locus_tag="PLP50_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPA53010.1"
                     /translation="MKEARITAVVLGAVLAVVLLSSSLYTVAETQQVIVTQFGKPVGK
                     PITTPGLKVKAPFVQVVHAFEKRFMEWDGDPNQIPTKDKRFIWVDCYARWRITDPLLF
                     FQRLRDERGAQSRLDDILDGETRNTIAKHELIDIVRSTNRPFVVGDDMADLSQPEPAE
                     NVEHGREKLTEEVLANARKRTHDLGIEILDFRIKQINYVEAVRQEVYARMISERKRIA
                     ERYRSEGAGEAARIAGEKERQLRVIESEAYRKAQEIRGKADAQAADIYAAAYNRDPDF
                     YRFLKSMNTLRESVDPETTLVLSTDGDLLRYLNAAK"
     gene            complement(3120..3722)
                     /locus_tag="PLP50_15570"
     CDS             complement(3120..3722)
                     /locus_tag="PLP50_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPA53011.1"
                     /translation="MSDTSRKRPATYEDILALPPHVTGQIVDGELFATPRPANPHATA
                     SSTLGMDIGGAYQRGRGGPGGWWIVFEPEIHLGRNVLVPDLAGWRRSRLPAIPPTAFF
                     TLAPDWVCEVLSPSTASFDRVKKMRVFAAEGVGHAWLVDPGARTLEVYRLAEGRWVLL
                     SAHEGTECVRAEPFEELEVVLSDLWVEMEPPTPQGATPAR"
     gene            3761..4939
                     /gene="aroC"
                     /locus_tag="PLP50_15575"
     CDS             3761..4939
                     /gene="aroC"
                     /locus_tag="PLP50_15575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPA53012.1"
                     /translation="MPRLRLLTAGESHGPLLTALLEGLPAGLRLDVAAIDANLARRQH
                     GYGRGGRMKIERDRVRFTGGLRGGETLGSPLAMTVENLDHGVWEKVMGPLEVDAEATA
                     KRRLSSPRPGHADLAGGLKYGRRDLRDVLERASARETAVRVAAGAVCRQLLAACGIEV
                     RSGVLSVGPVSVEEPREGFSFATLAKVDDASPFRAVDPSLEEKMTQAVDAAAKKGDTL
                     GGTILVAARGVPPGLGSHVSWDRKLDGRIGQAMLSIPSVKAVSIGSGVSNAFAPGSLA
                     HDPISWDEERGFFRERNRAGGLEGGITNGEDVLVVLFQKPIATLREGLPSVDLDTKAP
                     HRAQYERSDVTAVPACGVIGEAMLALVLADALLEKTGGDSMTEVRRNLEAFLAAQRAF
                     "
     gene            complement(4947..5333)
                     /locus_tag="PLP50_15580"
     CDS             complement(4947..5333)
                     /locus_tag="PLP50_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA53013.1"
                     /translation="MILDSSALVAVLLREPGWQALLGRIAADETAAVGAPTLAETGLV
                     LTARLGRDARRLLSAFVREAGITVVPFVEEHARVAVEAYARYGKGRHPAALNFGDCLT
                     YAVARLSGQPLLCAGDDFPRTDLPIA"
     gene            complement(5330..5599)
                     /locus_tag="PLP50_15585"
     CDS             complement(5330..5599)
                     /locus_tag="PLP50_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HPA53014.1"
                     /translation="MPLNIKDPVTEKLAAEVARLTGETKTGAIRRSLLERRERLALRV
                     IRPDREAGLVRFLEEEVWPLLPKGGSRRLTKRQREALLGYGPEGV"
     gene            5692..>6632
                     /locus_tag="PLP50_15590"
     CDS             5692..>6632
                     /locus_tag="PLP50_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPA53015.1"
                     /translation="MDFSRASLDREFPSRLSTIQMNHAAVSPLPARAADALREYADLL
                     STRGPLAFPELEARVKMLRAAGARLLGVTEELGGASSVSVVPNTTYGLALVAGGLDWR
                     PGDVVVTTESEFPANLAPWLDLARHGVEVRRIPTRDGAYRAEEVFAACDERTRLVSLS
                     LVAYHTGFVAPAAEVAAFCRARGIAFGLDAIQAAGAIPVDVAALGADYLSADGHKWML
                     GPEGCGLFFTTPAFRARLRAPSGWLNLKRTHPTQYELGANPKYTEDATKFEIGALPMP
                     GVFALHASIGLLLEAGLETVGRRIGGVLDLLAEGLPKV"
BASE COUNT          965 a         2420 c         2338 g          909 t
ORIGIN      
        1 tctgggtggc gcggaccggc tcgcgcgagc tcttcacgct ctgcgtcctg gcgaccgcga
       61 tcggcgtcgc ctacggctcg gcgaagctgt tcgacgtctc gttcgcgctc ggcgcgttct
      121 tcgccgggat gatgatgcgg gagtcggagt tcgcgcaccg cgcggccgac gagtcgttgc
      181 cgctccgcga cgcgttctcc gtcctcttct tcgtctccgt cgggatgctc ttcgacccgc
      241 gcgtcctcgt cgagcagccg gtcaaggtgc tcgtcgtcgt cgcgatcatc atgctcggca
      301 agacgctcgg ggcggtcggg ctcgtcctca cgatgcgata cccgctccac acggcgctga
      361 cggtcggggc ggcgctcgcg cagatcggcg agttcgcgtt catcatcgcc gtcctcgggc
      421 gcgacctcgg cctcctcccg gcggccgggc acagcctcat cctcgttggt tcgatcatct
      481 ccatcggcct gaaccccgtc gtcttctcca ccgtcgggcc gatcgccgag tggctgaagc
      541 ggcactcggc gctggcgcgc cggctggccg tcctcgagca cgaaacggcc gcgctcccga
      601 cgacgaccga cgaggcgctc ctgaccggcc aggtcgtcct cgtcggctac gggcgcgtgg
      661 ggcggcggat cgccgaggcg ctgacggagc ggcggatccc gttcgtcgtc gccgagatga
      721 gccgcgaggc cgtcgccgcg ctccgcgaga aggagatccc ggccgtctcg ggggacgcct
      781 ccgacccggc cgtcctcgtc caggcccatg tggcgaccgc ggcgatgctc gtcatcgcca
      841 cgcccgaccc ggtgaaggtg aggaagatgg tcgaggtcgc ccggacgctg aacccgtcga
      901 tcgagatcgt cctccggacc cacaccgagg aggaggccga gctcctccgg gaggagcagg
      961 ccggaaaggt cttcatgggc gagcacgagc tggcgctcgc gatggcgggg cacgtcctcg
     1021 cccggatgga ccgcccctcg gccgcctgag cccgcccgcc gaaaatcccg gattctccac
     1081 gcccgcggga atctctccgc gagccacggc gttgcatcgt ttcggcgccc acggctccgg
     1141 ccggccggcg cctcgcgaaa ggagacccga tggagatcca attccccgac gggacccggc
     1201 tacggactcc gagcatcccg gtccgcctcg tgacccgggc ggcactcggc gtcctcgcct
     1261 tcgtcgtcct cttctcggcc tggttcacga tcgagccgga agaggcgggg ctcgtgctga
     1321 ggttcggcaa gtacgcccgc accgtctcgc cgggcctcca cctgaagctc ccgagcccga
     1381 tcgagagggt cgtgaaggtg ccggtcgagc ggcagctgaa ggaggagttc gggttccgga
     1441 ccgaggcctc gggcgtgtcc cgctcgacct actcgcagga ggacctcacc tccgagtcgc
     1501 tgatgctgac gggcgacctg aacgtggcgg tcgtggagtg gacggcccag taccgggtcc
     1561 gcgacccgta caagttcctc ttcaaggtcc ggagcgtaca gaagacgttc cgcgacatga
     1621 acgagacggt catgcgcgag atcatcggcg accggagcgt gaacgaggtc ctgaccgtcg
     1681 gccggcagga gatcgccgcc gaggtcgagc agcggctgca ggacctctgc gaccagtacg
     1741 agaccggtat caaggtcgaa cagatcgtcc tgcaggacgt gaacccgccc gacccggtca
     1801 agccgtcgtt caacgaggtc aatcaggcac agcaggagcg cgagaagctg atcaaccagg
     1861 cccgcgccga ctacaaccag atcatcccgc gcgcgagcgg ccaggcccag cagacgatcg
     1921 agcaggccga gggcttcgcc accgaccgcg tcaaccgcgc ccgcggcgac gccgagctct
     1981 tcgtgaaggt ccacgccgcc taccagcgcg cccccgaggt gacccggcgc cggatgtacc
     2041 tcgagacgat gggcgagatc tacccgaagg tgaagaggaa ggtcgtcctg gacgagaagc
     2101 tgaagggggt ccttcccctg ctaccgctcg ccggcgcccc ggaggtgaag ccgtgaagga
     2161 agcccggatc accgccgtcg tcctgggcgc cgtcctcgcc gtcgttctcc tctcgagctc
     2221 gctctacacg gtggccgaga cgcagcaggt catcgtcacc cagttcggca agccggtcgg
     2281 caagccgatc acgacgcccg gcctgaaggt gaaggcgccg ttcgtccagg tcgtccacgc
     2341 cttcgagaag cggttcatgg agtgggacgg cgacccgaac cagatcccga cgaaggacaa
     2401 gcggttcatc tgggtcgact gctacgcccg gtggcgcatc accgacccgc tcctcttctt
     2461 ccagcgcctc cgcgacgagc ggggcgccca gtcgcgcctc gacgacatcc tcgacggcga
     2521 gacccggaac acgatcgcca agcacgagct gatcgacatc gtgaggtcga cgaaccgccc
     2581 cttcgtcgtc ggcgacgaca tggccgacct ctcgcagccg gagcccgccg agaacgtgga
     2641 gcacggccgc gagaagctga ccgaagaggt cctcgcgaac gctcggaagc ggacccacga
     2701 cctcgggatc gagatcctcg acttccggat caagcagatc aactacgtcg aggcggtgag
     2761 gcaggaggtc tacgcccgga tgatctccga gcggaagcgg atcgccgagc gctaccgctc
     2821 cgagggggcg ggcgaggccg cccgcatcgc gggcgagaag gagcgacagc tgcgggtcat
     2881 cgagtcggag gcgtaccgga aggcgcagga gatccgcgga aaggccgacg cgcaggccgc
     2941 cgacatctac gccgcggcct acaaccgcga ccccgacttc taccggttcc tgaagtcgat
     3001 gaacacgctc cgcgagagcg tcgacccgga gacgacgctc gtcctctcca ccgacggcga
     3061 cctcctccgc tacctgaacg ccgcgaagtg accgcggacg acgccggccc ggatctccgt
     3121 caccgggccg gcgtcgcgcc ctgcggagtc gggggctcca tctccaccca gaggtcggag
     3181 aggacgacct cgagctcctc gaacggctcg gcccggacgc actccgtccc ctcgtgtgcc
     3241 gagaggagga cccaccgccc ctctgcgagc cgatagacct caagcgtccg cgcgcccgga
     3301 tcgacgagcc aggcgtgccc gaccccctcg gccgcgaaga cgcgcatctt cttcacccgg
     3361 tcgaagctcg ccgtcgacgg cgaaaggacc tcgcagaccc agtcgggggc gagggtgaag
     3421 aaggcggtcg gcggaatcgc cgggagccgg ctccgccgcc agccggcgag gtcgggaacg
     3481 aggacgttcc ggccgaggtg gatctccggc tcgaaaacga tccaccagcc gcccgggccg
     3541 ccgcgcccgc gctggtaggc gccgccgatg tccatgccga gcgtcgagga ggccgtcgcg
     3601 tgagggttcg cggggcgcgg cgtcgcgaag agctcgccgt cgacgatctg ccccgtcacg
     3661 tgcggaggga gcgcgaggat gtcctcgtac gtcgcggggc gcttccggga ggtgtccgac
     3721 acggcgaggc gagattaccg ctccgctaga ctccccttcc atgccgcgcc tccgcctcct
     3781 gaccgccggc gagtcccacg ggccgctcct gaccgcgctc ctcgaggggc tccccgcggg
     3841 gctccgcctc gacgtcgcgg cgatcgacgc caacctcgcg cgccgccagc acggctacgg
     3901 ccgcgggggg cggatgaaga tcgagcgcga ccgggtccgc ttcacgggcg gcctgcgcgg
     3961 cggcgagacg ctcggctcgc cgctcgcgat gacggtcgag aacctcgacc acggcgtctg
     4021 ggagaaggtg atggggcctc tcgaggtcga cgccgaggcc accgcgaagc ggcgcctctc
     4081 ctcgccgcgg cccggccacg ccgacctcgc gggagggctc aagtacggcc ggcgcgacct
     4141 gcgcgacgtg ctcgagaggg cgagcgcgcg ggagacggcg gtgcgcgtcg ccgcgggcgc
     4201 cgtctgccgg cagctcctcg cggcatgcgg gatcgaggtg cggagcggcg tcctctccgt
     4261 cggcccggtg tccgtcgagg agccgcgcga gggattctcg ttcgcgacgc tcgcgaaggt
     4321 cgacgacgcc tcgccgttcc gcgcggtcga cccgtcgctc gaggagaaga tgacgcaggc
     4381 cgtcgacgcg gccgcgaaga agggcgacac gctcggcggg acgatcctcg tcgccgcgcg
     4441 cggcgtcccg cccggcctcg ggtcgcacgt ctcctgggac cgcaagctcg acgggcggat
     4501 cgggcaggcg atgctctcga tcccgtcggt gaaggcggtc tcgatcggct ccggcgtctc
     4561 gaacgcgttc gcccccggct cgctcgcgca cgacccgatc tcgtgggacg aggagcgggg
     4621 cttcttccgc gagcggaacc gcgcgggcgg gctcgaggga gggatcacga acggcgagga
     4681 cgtcctcgtc gtcctcttcc agaagccgat cgcgacgctc cgcgaggggc tcccctccgt
     4741 cgacctcgac acgaaggcgc cgcaccgcgc gcagtacgag cgttccgacg tgacggccgt
     4801 ccccgcctgc ggcgtcatcg gggaggcgat gctcgcgctc gtcctcgccg acgccctcct
     4861 cgagaagacg gggggcgact cgatgaccga ggtccgccgg aacctcgagg cgttcctcgc
     4921 ggcgcagcgc gcgttctgag ccgacctcag gcgatcggga ggtcggtcct cgggaagtcg
     4981 tctccggcgc agaggagcgg ctgccccgag agccgggcga cggcgtacgt caggcagtcc
     5041 ccgaaattca gcgccgccgg gtggcgcccc ttgccgtagc gggcgtacgc ctcgacggcg
     5101 acgcgggcgt gctcctcgac gaacgggacg accgtgatcc ccgcctcccg gacgaatgcg
     5161 gagaggagcc tccgggcgtc gcggccgagg cgagccgtca ggacgaggcc ggtctcggcg
     5221 agcgtcggag cgccgacggc ggcggtctcg tccgccgcga tgcggccgag gagcgcctgc
     5281 cagcccggct cccggaggag gacggcgacg agggcggagc tgtcgaggat cacacgccct
     5341 ccggcccgta gccgaggagg gcctcgcgct gcctcttcgt gaggcggcgg ctcccgccct
     5401 tcgggaggag cggccagacc tcctcctcga ggaaccggac gaggcccgcc tcccggtcgg
     5461 ggcggatgac ccggagcgcg aggcgttccc tccgctcgag gagcgagcgg cggatcgccc
     5521 cggtcttcgt ctcgcccgtg agccgggcca cctccgccgc gagcttctcc gtgaccgggt
     5581 ccttgatgtt cagcggcatg gagcgcctcc tcgatctggt accatggcac ccaaattcta
     5641 ccccttcctc tcgccggcgc gacccgtgcc gccggggggc ccggagggac gatggacttc
     5701 tcccgcgcct cgctcgaccg cgaattcccg tcgaggctct cgacgatcca gatgaaccac
     5761 gcggcggtct cgccgctccc ggcgcgcgcc gcggacgcgc tccgcgagta cgccgacctg
     5821 ctctccacga ggggcccgct cgcgttcccc gagctcgagg cgcgcgtgaa gatgctccgc
     5881 gcggcgggcg cgcgcctcct cggtgtgacg gaggagctcg gcggggcgtc gtcggtctcg
     5941 gtcgtcccga acacgaccta cggcctcgcg ctcgtcgcgg gcgggctcga ctggaggccg
     6001 ggcgacgtcg tcgtgacgac ggagtcggag ttcccggcga acctcgcgcc gtggctcgac
     6061 ctcgcgcggc acggcgtcga ggtgcggcgc atcccgacgc gcgacggcgc gtacagggcc
     6121 gaggaggtct tcgccgcctg cgacgagagg acgcgcctcg tctcgctctc gctcgtcgcc
     6181 taccacaccg gcttcgtcgc gcccgccgcc gaggtcgcgg cgttctgccg cgcgcgcggc
     6241 atcgccttcg gcctcgacgc gatccaggcg gccggggcga tcccggtcga cgttgccgcc
     6301 ctcggcgccg actacctctc ggccgacggc cacaagtgga tgctcggccc cgaggggtgc
     6361 ggcctcttct tcacgacgcc cgcgttccgg gcgcgcctgc gcgccccgtc gggctggctc
     6421 aacctgaagc ggacccaccc gacgcagtac gagctcggcg cgaaccccaa gtacacggag
     6481 gacgcgacga agttcgagat cggcgcgctc ccgatgccgg gggtcttcgc actccacgcc
     6541 tcgatcggcc tcctcctcga ggcgggcctc gagacggtcg gacggaggat cggcggcgtc
     6601 ctcgacctcc tcgccgaggg gctcccgaag gt
//
LOCUS       DAOONL010000165         6579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_13933_length_6579_cov_21.429798, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000165 DAOONL010000000
VERSION     DAOONL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13933_length_6579_cov_21.429798"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..111
                     /locus_tag="PLP50_15595"
     CDS             4..111
                     /locus_tag="PLP50_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA53016.1"
                     /translation="MLDAVGPIGGFNFQWAWATGRTAGEGAARAVLAGG"
     gene            complement(125..466)
                     /locus_tag="PLP50_15600"
     CDS             complement(125..466)
                     /locus_tag="PLP50_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HPA53017.1"
                     /translation="MSGRVVWSSGQGRICPTCGWPADDCRCSKKLDEPVPAKVTAKLR
                     IEKKGRGGKSVTVIDALPDNGPFLEELAKALKKACAVGGTVRPGAVELAGDVRDRVRP
                     LLTARGFVVKG"
     gene            602..1486
                     /locus_tag="PLP50_15605"
     CDS             602..1486
                     /locus_tag="PLP50_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA53018.1"
                     /translation="MRTEELEIRAGDGFSLAATLHHPEAPEEESVVLVNAGTGIRRGF
                     YARFAAFLASCGFPVLTWDYRGIGDSRPASLRGFRAAMRDWGREDLEALLGWVGERHP
                     GRPILVVGHSAGGQILGLAPSASRISAVLAVAAQSGWYRHWPVPRRYLMAGLWWGLMP
                     AATALCGYFPSRALGLGEDLPKGVALEWARWCRNPQYMVDASGAPLRPHFADLRAPVL
                     AFSFADDPFAPRAAVEQLLSFYTEANVTHRHVVPSEIGLRGVGHFGFFRETCREVLWK
                     ECVDWLRRRGAAAERGVA"
     gene            1483..2754
                     /locus_tag="PLP50_15610"
     CDS             1483..2754
                     /locus_tag="PLP50_15610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53019.1"
                     /translation="MKNVSPEPAPVPEDAQEERRGPVAVPAAAPSRARRLFLDALSEH
                     LTGARVVFRTEDGRHEVGRGDAVAAVVRVHRERFFERALAQGNLGLGESFMDGDWEVE
                     QGTLEGLLEALLRARLDHKLAGNPGVALKVLALQLLNGLRGRQWAHVQRHYDVGDDLF
                     ELFLDRSLVYSCAYLVKEDDSIEAMQEQKLDRICRKLGLAPGERLLDIGCGFGGLLIH
                     AASRFGVTGVGITTSARHAAKGMERVRAAGLEGSVSLELRDHRTVEGRFDKVVSVGMM
                     EHLPRGEYARYFERIAAVLTPEGRGLVHTIGCNAARNRHDPFIQKYVFPGSGQPKLSE
                     MAAECERHGLAILDVENMVRHYAHTARRWLDRFRERAHLLDPKRYDASFRRMWEYYLC
                     CCIAGARASDGALYQVLFARDYAAEMPLHRV"
     gene            2768..3850
                     /locus_tag="PLP50_15615"
     CDS             2768..3850
                     /locus_tag="PLP50_15615"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPA53020.1"
                     /translation="MNALSVLNQRWVEAGYTRKPVGVHLLQLAAHVAFALGGTALYFL
                     VPGLAAKALGLLVSSYGMIGISTHAHTSSHNAIFDATPPNRGLVHLGFPFLCGVSALY
                     WWNKHVHVHHPTPNVVGLDDDVDLMPFFALTREEAERGGPLLKLWFRVQGIAIVPILA
                     LNCFNIQKTGWSYWLRTLRGPGAKRGAHALDLALLLGHYVFWIGLPALFFPLGEVVLF
                     YVVRMLLLGYGMFAVFAPAHFPARASFVSAEGETNRLAYKKSKDFILLQTATTVNFRT
                     GPIGRLLCLGVEYQIEHHLFPGISHVHYPRLSVELKRFCEENGYPYRTLGWWEGIVES
                     IRAFRHLKPVEPRLEELRLPAEGQAA"
     gene            3847..4734
                     /locus_tag="PLP50_15620"
     CDS             3847..4734
                     /locus_tag="PLP50_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPA53021.1"
                     /translation="MRAEHGDGALRRLGAALDRRWARSARPSRGEEIELAAGRLRVRR
                     GGGPDGVPVVVVPDPPNVVEHYDGVFDALAAGGPVAVVEAPGFGFSAPREGARYRIEE
                     LSSALEELLLRIGFRGATLSLACVGAFAAVDLAARRPDLVGRLVLSQVASRREMASWV
                     RRKDVCGVIQTPLLGPVAVRAARRRIARWWYRAALPPGADPGPFAAPALAALRAGAAF
                     PLASAFQEFARFPGDRLRPACPVTVLLGTADPTHAGTELGSVAEELPGARVLVFPRSG
                     HFPDLENPGEWLAALRAPA"
     gene            complement(4717..>6579)
                     /locus_tag="PLP50_15625"
     CDS             complement(4717..>6579)
                     /locus_tag="PLP50_15625"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HPA53022.1"
                     /translation="RRRRVPVSLSIPEILSTTRLFGGLAPEDLARIGPEFEPVRVPGG
                     ETVFEHGETGDCLYIVASGRIEVSLTLPDGSLRPIGEAGPGECLGEMALLTGERRSAT
                     ARSRRDTDLLRLSGEAFRRLLETHPRLMHGLTRRIIGHYQSVLAGRRGASAGRISTIA
                     VATTQKDGRVAEFTRRLVEALGKHGSVRHLDASALDSALGEGSAQAARDSSRNSGIVA
                     YLNEQEEKHRFVVYEAEPGPSSWTSRCLRQADRILFVAPDDGSPAPGPIEALLARQGL
                     ADVRTADLVLLHRETKPLYLGTSTWLAPRRVRAHHHVAESAPGEMERLVRSLTGEVRG
                     LVLGGGGARSFAQIGVLRALAERGVSFDVVGGTSMGAFLGAQVAMGWDARKMLTFNEV
                     TWTRTKPLKDYTFPYLGFVTGRRFYSVARAIYGEARLEDLRIPFFCCSSNLTRARVEV
                     HDAGPAWRALGASIAVPGLAPPAFEGGDLLVDGAVLANLPVDVMRGRCSGTVVTIDVS
                     PVEDLSVDPALTMAPNSWGLLFRKLLGKKPQVPGFFELLMRSSGLASVQQVDEMQRLS
                     DVFFHPPIEKYSIFDFSLIRELERVGYEHAAQVLASPAGEALARADAPASGGSA"
BASE COUNT          855 a         2424 c         2324 g          976 t
ORIGIN      
        1 gagatcctcg acgccgtcgg cccgatcggc ggcttcaact tccagtgggc ctgggcgacg
       61 gggcggacgg cgggagaagg agcggcgcgg gccgttctcg cgggcggctg agccgcgctc
      121 gcgatcaacc tttcacgacg aagccacgcg cggtgaggag cggacgcacg cgatcgcgca
      181 cgtctcctgc gagctcgacc gcgccgggac ggaccgtccc gccgaccgcg cacgccttct
      241 tcagcgcctt cgcgagctcc tcgaggaacg gcccgttgtc ggggagcgcg tcgatcaccg
      301 tcacgctctt tccgccgcgg cccttcttct cgatccggag cttcgccgtc accttcgcgg
      361 ggaccggctc gtcgagcttc ttcgagcagc ggcagtcgtc ggccggccag ccgcacgtcg
      421 ggcagatccg cccctgaccg ctcgaccaga cgacgcgtcc gctcacggga ggatcttctc
      481 agacggagcg gatccggcct tcggccgtga ctggcctcac gggaaattga gatagacttg
      541 caactaatcc gacgggacac cagattgacg tttcgaggca ccccctcgga tgacgtttgg
      601 catgcgcacg gaagagctcg agatccgcgc cggagacggc ttttcgctgg cggcgactct
      661 ccatcacccg gaggcgccgg aggaggaatc cgtcgtcctc gtgaacgccg gaaccgggat
      721 tcgccgcggc ttttacgcgc gattcgcggc cttcctcgcg tcgtgcggct tcccggtcct
      781 gacgtgggac taccgcggga tcggcgattc gcgtcccgcc tcgctccgag ggtttcgcgc
      841 ggcgatgcgg gactgggggc gcgaggacct cgaggcgctt ctcggctggg tcggcgagag
      901 gcacccgggc cgtccgatcc tcgtcgtcgg ccacagcgcc ggagggcaga tcctcggtct
      961 cgccccgtcg gcctcgcgga tctccgcggt cctcgccgtc gccgcgcaga gcggctggta
     1021 ccggcactgg cccgtgccgc gccggtacct gatggccggc ctctggtggg ggctcatgcc
     1081 ggcggccacg gcgctctgcg gctacttccc gtcgcgggcg ctcggcctcg gcgaagacct
     1141 gccgaagggg gtcgccctcg agtgggcccg ctggtgccgg aatccgcagt acatggtcga
     1201 cgcgagcggc gccccccttc ggcctcattt cgccgacctg cgcgctccgg tcctcgcctt
     1261 cagcttcgcc gacgacccgt tcgcaccgcg cgcggccgtc gagcagctgc tctccttcta
     1321 cacggaagcg aacgtcaccc atcgccacgt cgtcccgtcg gagatcggcc tccggggcgt
     1381 cggccatttc ggcttcttcc gagagacgtg ccgcgaggtg ctctggaagg agtgcgtcga
     1441 ctggctgcgg cgccggggcg ccgcggccga aagaggagtc gcatgaagaa cgtctctccg
     1501 gagcccgcgc cggtccccga ggacgcgcaa gaggagcgcc gagggcccgt ggccgttccc
     1561 gccgccgcac cgtcgcgggc gcgtcggctc ttcctcgacg cgctctcgga gcatctcacc
     1621 ggcgcgcggg tcgtcttccg gacggaggac ggccggcacg aggtcggccg gggcgacgcg
     1681 gtcgccgccg tcgtgagggt ccaccgcgag cgcttcttcg agagggctct cgcccagggg
     1741 aacctcgggc tcggcgagtc gttcatggac ggggactggg aggtcgagca ggggacgctc
     1801 gaagggctcc tcgaggcgct cctgcgggcc cggctcgacc acaagctcgc cggcaacccg
     1861 ggcgtcgcgc tgaaggtcct cgcgctccag ctcctgaacg gcctgcgggg ccggcagtgg
     1921 gcgcacgtcc agcgccacta cgacgtcggc gacgacctct tcgagctctt cctcgaccgc
     1981 tccctcgtct actcctgcgc gtacctggtg aaggaggacg actcgatcga ggcgatgcag
     2041 gagcagaagc tcgaccggat ctgccggaag ctcggcctgg cgccaggcga gcggctcctc
     2101 gacatcggct gcggcttcgg cggcctcctg atccacgccg cctcgcgatt cggtgtgacg
     2161 ggcgtcggca tcacgacgag cgcgcgccac gcggcgaagg ggatggagcg ggtccgcgcg
     2221 gcggggctcg aggggagcgt ctccctcgag ctgcgcgacc accggaccgt cgaggggcgc
     2281 ttcgacaagg tcgtcagcgt cgggatgatg gagcacctgc cgcggggcga gtacgcgcgc
     2341 tacttcgagc ggatcgcggc ggtcctcacg cccgaggggc gcggcctcgt ccacacgatc
     2401 ggctgcaacg ccgcgcggaa ccgccacgac cccttcatcc agaagtacgt cttccccggc
     2461 tcggggcagc cgaagctctc ggagatggcg gcggagtgcg agcggcacgg cctcgcgatc
     2521 ctggacgtcg agaacatggt gcggcactac gcgcacacgg cccggcgctg gctcgaccgc
     2581 ttccgcgagc gggcgcacct cctcgacccg aagcgctacg acgcctcgtt ccgtcggatg
     2641 tgggagtact acctctgctg ctgcatcgcc ggggcgcgcg cctcggacgg cgcgctctac
     2701 caggtgctct tcgcccgcga ctacgcggcc gagatgcccc tccaccgcgt ctgaggaggc
     2761 cgagaccgtg aacgccctct ccgtcctgaa ccagcggtgg gtcgaggccg gctacacgcg
     2821 caagcccgtc ggcgtccacc tcctccagct cgcggcccac gtcgcgttcg cgctcggggg
     2881 cacggcgctc tacttcctcg tgccggggct cgccgcgaag gcgctcgggc tgctcgtctc
     2941 ctcctacggg atgatcggca tctcgacgca cgcgcacacc tcctcgcaca acgcgatctt
     3001 cgacgcgacg ccgccgaacc ggggcctcgt ccacctcggc ttcccgttcc tctgcggcgt
     3061 ctcggcgctc tactggtgga acaagcacgt ccacgtccac cacccgacgc cgaacgtcgt
     3121 cggcctcgac gacgacgtcg acctgatgcc gttcttcgcg ctcacgcgcg aggaggccga
     3181 gcgtggcggt ccgctcctga agctctggtt ccgggtccag gggatcgcga tcgtcccgat
     3241 cctcgccctc aactgcttca acatccagaa gacgggctgg tcgtactggc tccggaccct
     3301 ccggggtccc ggggcgaagc ggggcgccca cgcgctcgac ctcgccctcc tcctcggcca
     3361 ctacgtcttc tggatcggcc tcccggcgct gttcttcccg ctcggcgagg tcgtcctctt
     3421 ctacgtcgtc cggatgctcc tcctcggcta cgggatgttc gccgtcttcg ctccggccca
     3481 cttcccggcg cgggccagct tcgtctcggc ggagggggag acgaaccggc tcgcctacaa
     3541 gaagtcgaag gacttcatcc tcctccagac ggcgacgacg gtgaacttcc ggaccggtcc
     3601 gatcggccgc ctcctctgcc tcggcgtgga gtaccagatc gagcaccacc tgttccccgg
     3661 gatctcgcac gtccactacc cgcggctctc ggtggagctg aagcgcttct gcgaggagaa
     3721 cggctacccg tatcggacgc tcggctggtg ggaggggatc gtcgagtcga tccgggcgtt
     3781 ccggcacttg aagccggtcg agccgcgcct cgaggagctt cgcctgccgg cggagggaca
     3841 ggcggcgtga gagcggagca cggcgacggg gcgctccggc gcctcggcgc cgcgctcgac
     3901 cgccgttggg cccgctccgc acgcccgtca cgcggagagg agatcgagct cgccgccggg
     3961 cgcctccgcg ttcgccgcgg gggcgggccc gacggcgtcc cggtcgtcgt cgtccccgac
     4021 ccgccgaacg tcgtcgagca ctacgacggc gtcttcgacg ccctcgcggc cggcggcccc
     4081 gtggcggtcg tcgaggcgcc cggattcggg ttctcggcgc cccgggaggg agcgcgctac
     4141 cgcatcgagg agctctcctc cgcgctcgag gagctcctcc tgcgcatcgg cttccgcggc
     4201 gcgaccctct cgctcgcctg cgtcggcgcg ttcgcggcgg tggacctcgc ggcgaggcgg
     4261 ccggacctcg tcggccgcct cgtcctctcg caggtcgcgt cgcggcgcga gatggcgtcg
     4321 tgggtgaggc gcaaggacgt ctgcggggtc atccagacgc cgctcctcgg cccggtcgcg
     4381 gtccgggcgg cgcgccggcg gatcgcgcgg tggtggtacc gcgccgccct gccgcccggg
     4441 gccgaccccg gcccgttcgc cgcacccgcg ctcgccgcgc tccgagccgg ggcggcgttc
     4501 ccgctcgctt cggcgttcca ggagttcgcg cgctttccgg gcgaccgcct ccggcccgcc
     4561 tgccccgtca ccgtcctcct cgggacggcc gaccccactc acgcggggac ggagctcggc
     4621 tcggtcgcgg aggagctccc gggcgcgcgc gttctcgtct ttccccgctc ggggcacttc
     4681 cccgacctcg agaaccccgg ggagtggctc gccgccctac gcgctcccgc ctgaagccgg
     4741 tgcgtccgct cgtgcgaggg cctcgccggc cggagaagcg aggacctggg ctgcgtgttc
     4801 gtagccgacc cgctccagct cccggatgag cgagaagtcg aagatcgagt acttctcgat
     4861 cggggggtgg aagaagacgt ccgacaggcg ctgcatctca tccacctgct ggacgctcgc
     4921 aagcccggac gagcgcatga ggagctcgaa gaagccgggc acctgcggct tcttcccgag
     4981 gagcttgcgg aagagaagcc cccaggaatt cggcgccatc gtcagcgccg ggtcgacgct
     5041 caggtcctcc acgggactga cgtcgatcgt gacgacggtc ccggagcagc ggccgcgcat
     5101 cacgtcgacg gggagattcg cgaggacggc gccgtcgacg aggaggtcgc ccccctcgaa
     5161 ggccggtggc gcgaggcccg gcaccgcgat gctcgcgccg agggccctcc aggcgggccc
     5221 ggcgtcgtgc acctcgacgc gcgcccgcgt caggttgctc gagcagcaga agaacgggat
     5281 ccggaggtct tcgagacgcg cctcgccgta gatcgcgcgg gccacggagt agaaacgccg
     5341 cccggtgacg aagccgagat acggaaacgt gtagtccttc agcggcttcg tccgggtcca
     5401 cgtcacctcg ttgaacgtga gcatcttccg cgcgtcccac cccatcgcga cctgcgcgcc
     5461 gaggaacgcg cccatgctcg tcccgcccac gacgtcgaac gagacgcccc gttccgcgag
     5521 cgcccggagg acgccgatct gcgcgaacga tcgggctccg ccgccgccga ggacgaggcc
     5581 gcgcacctcg cccgtcagcg accggacgag acgctccatc tcgcccggcg cgctctcggc
     5641 gacgtggtga tgggcccgga cgcgccgcgg cgcgagccac gtgctcgtcc cgagatagag
     5701 cggtttcgtc tcgcggtgga ggaggacgag gtcggcggtc ctcacgtcgg cgagaccctg
     5761 gcgggcgagg agcgcctcga tcggcccggg agcggggctc ccgtcgtcgg gcgcgacgaa
     5821 gaggatccgg tcggcctggc ggagacagcg gctcgtccag gacgaggggc cgggctccgc
     5881 ctcgtagacg acgaagcggt gcttctcctc ctgctcgttc aggtacgcca cgatcccgga
     5941 gttgcgcgag gagtcgcggg ccgcctgggc ggagccctcg ccgagggcgc tgtcgagcgc
     6001 ggacgcgtcg aggtgccgga cgctcccgtg ctttccgaga gcctcgacga gccgccgggt
     6061 gaattccgcg acccgcccgt ccttctgcgt cgtggcgacc gcgatcgtcg agatccgccc
     6121 ggcggaggcg ccgcgccggc cggcgaggac cgactggtag tggccgatga tcctccgcgt
     6181 cagcccgtgc atgaggcgcg gatgcgtctc gaggagccgc cggaacgcct cgcccgacag
     6241 gcggaggagg tcggtgtcgc gtctcgaccg ggcggtcgcg ctccgccgct cgcccgtcag
     6301 gagcgccatc tcgccgaggc actctccggg gccggcctcg ccgatcgggc gcagcgagcc
     6361 gtcggggagc gtcagcgaca cttcgatccg gccgctcgcg acgatgtaga ggcagtcgcc
     6421 cgtctccccg tgctcgaaga cggtctcgcc tccggggacc cggaccggct cgaactccgg
     6481 gccgatgcgc gcgaggtctt ccggggcgag ccccccgaac aggcgcgtcg tcgacaggat
     6541 ctcgggaatc gacaggctca cgggcaccct ccgtcggcg
//
LOCUS       DAOONL010000166         6533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_14068_length_6533_cov_18.142791, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000166 DAOONL010000000
VERSION     DAOONL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_6533_cov_18.142791"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLP50_15630"
     CDS             complement(<1..734)
                     /locus_tag="PLP50_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA53023.1"
                     /translation="MTKPRVRKLFLAVAALAVAAGGTAFAVLARDRREVTTRSAEAYR
                     FYRQGLENERRLYHKDALASYAKALELDPHFAMATVRLAGLLAGRDRDRAKSLVQCAS
                     QSREVLNERERLTLEIVEAELLAGEPKKAAERVREYVRRFPEDPEGYFRLANVLAREG
                     KPDETLATYERLVALDPNQAIAYNYLGYYFLRRRDFVKGEDFFKRYRFLAPDQANPYD
                     SLGELYANTGRYAEAEEHLKRALEIKP"
     gene            complement(772..3336)
                     /locus_tag="PLP50_15635"
     CDS             complement(772..3336)
                     /locus_tag="PLP50_15635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA53024.1"
                     /translation="MTPAPPAARRRAIVVVGILGALIAAMSVADMFLPKAWDGVVPDT
                     YAESGLQVRAVVRGGPAEGAGIRPGDTILGIGRRMLNTPAEAASELGRLAAGETVPYL
                     VKRGEQVLEREVLLSPYRLGSASYVYYALLGGLFFGLGLFVYSRRAGDEAAEVFFLLC
                     VLFMLFFVCRLRPSSYYWIDWFVQVAGTLALFLLGAVFLHFFLLFPRRKVFRFAEARP
                     GEDPPAAPLVYLQRFLNGSPALFVLLYTLPPAFYALQMAVLRRTGDRSALVYGAPRAN
                     WILLADYLVLGLLALAHSWWTAREKTTRRPILVLLLGTLGGIVPFVLFAVYFPSLFRD
                     ERYLAWGVVPMALVPLTFAYSIVRFRLFDVQLIVGKSIVYAILTAIVTGLYALAVVAG
                     NALVSSRTLSPLFAFAFGLAVVLLVDPLRRRMQAVVDRLFFRDRADFQSAFRDISRQV
                     VAQLDRGKMQQLLTERTAELLRLSRLDLLTPRPEDGALADPRRGDVSLPVGAALGRLL
                     VERDAPVPLRDLDPWALDAPSRSFLKAQLERGARVLVPVATRGRLLGVLAAGEKKSEE
                     EFHKEDLDNLVTIANQGALGLEVAALHEQLTRRAEVERDLEIARDIQVSLFPRELPRI
                     AGIELYGVSLPAKVVGGDFYDFLPVDGGFDGRVALVVGDVSGKSIPASLLMVAAKEIV
                     YARAMQDPEPSTVFRESNRRIYEIKRRMFVSLGYFLYDPVQLTLHYSIGGQPLPLVVR
                     GGEAVELPAPVSRLPLGALRETTYDAKTFWLRRGDLLLFYTDGLNEAMSVESAYFGDE
                     RLKASLVRHSAAPLPEMAEEILEDIRQFTFGAEQSDDATFVLLRVTGAKPAPPGSV"
     gene            3446..4081
                     /gene="tmk"
                     /locus_tag="PLP50_15640"
     CDS             3446..4081
                     /gene="tmk"
                     /locus_tag="PLP50_15640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPA53025.1"
                     /translation="MTARSRRGLFVTFEGIEGSGKSTQLRILARRLRRLGVPLLVTRQ
                     PGGTPAGARLRRLLLHAGRIDPRAELLLMFADRRQHLTESIEPALAAGTLVLCDRYTD
                     ASRAYQGAGRGLGEDLVDLLHARFCGLEPDRTYLFDCPPATGLARVAAARGGKDRIER
                     EALAFHRRVRAAYLARARKEPGRFLVLDGTRPVEELAERVAGDLDTLRAAR"
     gene            4078..5037
                     /locus_tag="PLP50_15645"
     CDS             4078..5037
                     /locus_tag="PLP50_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53026.1"
                     /translation="MTDVPVQLLTGRGSKRLEAEALDVAASFLCRSGDRTASCPDCRR
                     VARREHPDLLVAAPESGRRANTPPFDETSGSKETTIPTALVRAISADASRLPYEGTRR
                     VLVLLDVDRTEAAAFSALLKILEEPPSRARFVLTATRPRLLPPTILSRLVLRRLPALS
                     REALVAALTGGGMSPEEAAGRAAFGPPGEEEARDLDLAAAREGRDALLEAVSGLLLSR
                     STGWALVLAGRLAAEDAGATADRLSLLAGLLRDAVAAGTDPAGRTVHHVERYRDLARL
                     SEAGDTVLLEAARRALQAAGDLPDSRRNPRLAAEAFALSLRAL"
     gene            complement(5098..>6533)
                     /locus_tag="PLP50_15650"
     CDS             complement(5098..>6533)
                     /locus_tag="PLP50_15650"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPA53027.1"
                     /translation="GPPVPRGGRAAAEAAMTHPRLSSTSSRAKTWSRPGLAGALLALP
                     ALLVATSAVAQREPGTASPDTGSAPAPSYSAPAPSYSAPSPSHSSPSPSYSAPAPSAP
                     RSAPAPSAGSPSTPRRATAPRESTSGTPARPGRRVVGAEPRDGRSGGPTTATAGGGGS
                     RVPRIVPTHPRGSGSGGHHHPRRRYYSPYAGWGGTSWWWGGPWDWWWWGSRFGPYHWG
                     FGGVVIVEDYSPDPYRYARVDTDVSPEAAEVYLDGTFIGSADDFDGYPDFLWLEEGKY
                     TLEFRHPSYETVKHDLEVEPGQSLRFRDDLKLEPGKRRLEAVEPPRRGTPHGRVFGKP
                     EEPRRDRTGRFEARAEADRERDLEELEDLEELEELAEELDEADEDLAPAPKVRGEVKE
                     GRSGREALPAERSRLRFEIEPSDAAVYLDDDYVGTGKELAGLSRGVPVDAGKHTVSVV
                     RPGFVTKTIEVEVKPGVAIDVVVELEK"
BASE COUNT          914 a         2363 c         2412 g          844 t
ORIGIN      
        1 ggcttgatct cgagcgcccg cttcaggtgc tcctccgcct cggcgtagcg gcccgtgttc
       61 gcgtagagct ccccgaggga gtcgtagggg ttcgcctggt cgggggcgag gaagcggtag
      121 cgcttgaaga agtcctcgcc cttcacgaag tcgcggcgcc ggaggaagta gtagccgagg
      181 tagttgtaag cgatggcctg gttcgggtcg agcgcgacga ggcgctcgta ggtggcgagc
      241 gtctcgtccg gcttcccttc gcgggccagg acgttcgcga gacggaaata gccctcgggg
      301 tcttccggga agcgccggac gtactcccgg accctttccg ccgccttctt cggctcgccg
      361 gccaggagct ccgcctcgac gatctcgagg gtcagccgct cgcgctcgtt caggacctcg
      421 cggctctggg acgcgcactg gacgagcgac ttcgcccggt cccggtccct cccggcgagg
      481 aggccggcca ggcgcacggt cgccatcgcg aagtgagggt cgagctccag cgccttcgcg
      541 taggaggcga gggcgtcctt gtggtagagc cggcgctcgt tctcgagccc ctgccggtag
      601 aagcggtagg cctccgccga ccgggtcgtc acctcgcgcc ggtcccgtgc gaggacggcg
      661 aacgcggtcc ccccggcggc cacggcgagg gccgcgaccg cgaggaagag ctttcggacg
      721 cgcggtttcg tcacggactg gtcccttccg ggccaccggg ccctcggaaa gctatacgga
      781 ccccggaggt gcgggtttcg cccccgtcac ccgcaggagg acgaacgtgg cgtcgtccga
      841 ctgctcggcc ccgaacgtga actggcggat gtcctccagg atctcctccg ccatctccgg
      901 gaggggtgcc gcggagtggc ggacgaggga ggccttcagg cgctcgtcgc cgaagtaggc
      961 gctctccacc gacatcgcct cgttcaggcc gtcggtgtag aagaggagga ggtcgcctct
     1021 ccggagccag aacgtcttcg cgtcgtacgt cgtctcgcgg agggcgccga gcgggagccg
     1081 gctgaccggc gccggcagct cgacggcctc gccgccacgg acgacgagcg ggagaggctg
     1141 ccctccgatg gagtagtgga gggtgagctg gaccgggtcg tagaggaaat agccgagcga
     1201 gacgaacatc ctccgcttga tctcgtagat gcggcggttc gactcgcgga agacggtgga
     1261 cggctccggg tcctgcatcg cccgggcgta gacgatctcc ttggccgcga ccatcaggag
     1321 cgacgcgggg atcgacttgc cggagacgtc gccgacgacg agcgcgaccc gcccgtcgaa
     1381 cccgccgtcg acgggaagga aatcgtagaa gtcgccgccg acgaccttcg ccgggaggct
     1441 gacgccgtag agctcgatcc cggcgatccg ggggagctcc cgcgggaaga ggctcacctg
     1501 gatgtcgcgg gcgatctcga ggtcacgctc gacctcggcc cggcgggtca gctgctcgtg
     1561 gagggccgcc acctcgaggc cgagggcccc ctggttcgcg atcgtcacga ggttgtcgag
     1621 gtcctccttg tggaactcct cctccgactt cttctctccg gccgcgagga cgccgaggag
     1681 ccggccgcgc gtcgcgaccg ggacgaggac gcgcgccccg cgctcgagct gggccttcag
     1741 gaagctccga gagggggcgt cgagcgccca ggggtcgagg tcgcgcaggg ggaccggcgc
     1801 gtcccgctcc acgaggagcc gcccgagcgc ggcgccgacc gggagcgaga cgtctccccg
     1861 ccgcgggtcg gcgagcgccc cgtcctcggg acggggcgtc agcaggtcga gccggctcag
     1921 ccggaggagc tccgccgtcc tctcggtgag gagctgctgc atcttgccgc ggtcgagctg
     1981 ggcgacgacc tggcggctga tgtcgcggaa cgccgactgg aagtcggccc ggtcgcggaa
     2041 gaagagccgg tccacgaccg cctgcatcct ccgccggagc gggtcgacca gcaggacgac
     2101 cgcgaggccg aacgcgaacg cgaagagcgg cgagagggtc cgcgaggaga cgagggcgtt
     2161 ccccgccacg acggcgaggg cgtagaggcc cgtgacgatc gccgtgagga tcgcgtagac
     2221 gatcgacttg ccgacgatga gctgcacgtc gaagagccgg aacctgacga tcgagtacgc
     2281 gaaggtgagc gggacgaggg ccatcggcac gacgccccag gcgagatagc gctcgtcccg
     2341 gaagagcgac gggaagtaga ccgcgaagag gacgaacggg acgatcccgc cgagcgtgcc
     2401 gagcagcagg acgaggatcg gccgccgggt cgtcttctcg cgcgccgtcc accaggagtg
     2461 ggcgagcgcg aggaggccga ggacgaggta gtcggcgagg aggatccagt tcgcccgggg
     2521 cgcgccgtag acgagcgcgg agcggtcgcc ggtcctccgg aggaccgcca tctggagcgc
     2581 gtagaacgcc ggcgggagcg tgtagaggag gacgaagagc gcgggcgacc cgttcaggaa
     2641 cctctgaagg tagacgagcg gcgccgccgg cgggtcctct cccggccgcg cctcggcgaa
     2701 gcggaagacc ttccggcgcg ggaagaggag gaagaagtgg aggaagaccg cgccgaggag
     2761 gaagagggcg agcgtccccg ccacctgcac gaaccagtcg atccagtagt agctggacgg
     2821 ccgcaggcga cagacgaaga agagcatgaa gaggacgcag aggaggaaga agacctccgc
     2881 cgcctcgtct ccggcccgcc tcgagtagac gaagaggccg agaccgaaga agagcccgcc
     2941 gaggagggcg tagtagacgt acgaggccga cccgagccgg tagggcgaaa ggaggacctc
     3001 gcgctcgagg acctgctctc cacgcttgac gaggtacggg acggtctcgc ccgccgccag
     3061 ccggccgagc tccgaggccg cctcggccgg cgtgttcagc attcgccgcc cgatgccgag
     3121 gatcgtgtcc cccgggcgga tgcccgcccc ctccgcggga ccgccgcgga cgacggcgcg
     3181 gacctggagc ccgctctcgg cgtaggtgtc cggcacgaca ccgtcccatg ccttcggcag
     3241 gaacatgtcg gccaccgaca tcgccgcgat cagcgcgccg aggatcccga cgacgacgat
     3301 ggccctccgg cgggctgcag gcggcgcggg ggtcacggca ctcttctctc gatcggctct
     3361 cgcgtcaggg gggcgcacga agtatacgga ggccatcccg gccggttcgg cgagccgtgc
     3421 cgcctgcatg cgagaatcgc gcctcgtgac cgcccgcagc aggcggggcc tcttcgtcac
     3481 cttcgagggg atcgagggct ccggcaagtc gacccagctc cgaatcctcg cccggcgcct
     3541 ccgccggctc ggcgtgccgc tcctcgtgac ccgccagccg ggcggaactc cggccggagc
     3601 ccgcctgcgg cggctcctcc ttcacgccgg ccggatcgac ccgagggccg agctcctcct
     3661 gatgttcgcc gaccggcggc agcacctgac ggagtcgatc gagccggcgc tcgccgccgg
     3721 cacgctcgtc ctctgcgacc gctacaccga cgcctcgcgg gcctaccagg gggcgggacg
     3781 tggcctcggg gaggacctcg tcgacctcct ccacgcgcgc ttctgcgggc tcgagcccga
     3841 ccggacgtac ctcttcgact gcccgcccgc gacggggctc gcccgcgtcg ccgccgctcg
     3901 cggggggaag gaccggatcg agcgcgaggc gctcgcgttc caccggcgcg tccgggccgc
     3961 ctacctcgcc cgggcgcgga aggagccggg ccgcttcctc gtcctggacg ggacccgccc
     4021 cgtcgaggag ctcgcggagc gggtcgccgg cgacctcgac acgctccggg ccgcgcgatg
     4081 accgacgtcc cggtccagct cctgacgggg cgcggctcga agcgtctcga ggcggaggcg
     4141 ctcgacgtcg ccgcgtcgtt cctctgtcgg agcggcgacc ggacggcttc ctgccccgac
     4201 tgccgtcgcg tggcgcgccg cgagcacccg gatctcctcg tcgccgcccc ggaaagcggc
     4261 cggcgcgcga acacccctcc gttcgacgag acctcgggct cgaaggagac gacgattccg
     4321 acggcgctcg tccgcgcgat ctcggccgac gcctcgcgcc tgccgtacga agggacgcgc
     4381 cgcgtcctcg tcctcctcga cgtcgaccgg acagaggcgg ccgcgttcag cgcgctcctg
     4441 aagatcctcg aggagccgcc gtcgcgcgcc cgattcgtcc tgaccgcgac gcgtccccgt
     4501 ctcctcccgc cgacgatcct ctcgcggctc gtcctccgcc ggcttcccgc gctctcccgg
     4561 gaggcgctcg tcgccgccct caccggcggc gggatgtctc ccgaggaagc ggccgggcga
     4621 gccgccttcg gcccgcccgg cgaggaggag gcacgcgacc tggacctcgc cgccgcgcgg
     4681 gagggccggg acgccctcct cgaggcggtc tcggggctcc tcctgtcccg ttcgacgggc
     4741 tgggccctcg tcctcgccgg ccggctcgcc gccgaggacg cgggcgcgac cgcggaccgc
     4801 ctctcgctcc tcgcggggct cctccgggac gcggtcgccg ccggcaccga cccggccggc
     4861 cggaccgtcc atcacgtcga acggtaccgg gacctcgccc gcctctccga agccggggac
     4921 acggtcctcc tcgaggcggc gcgccgggcg ctccaggcgg ccggggacct tcccgactcg
     4981 cgccggaacc cgcgcctcgc cgccgaggcc ttcgccctct cgctgcgggc cctttgaaac
     5041 gcgagacggg cggggtgccc ccaagggtgc gccccgcccg gacggaggag gtgaccgtta
     5101 cttctcgagc tcgacgacga cgtcgatcgc gacccccggc ttcacctcga cctcgatcgt
     5161 cttcgtgacg aagcccgggc ggacgacgga gacggtgtgc tttccggcgt cgaccgggac
     5221 gccgcggctc aggccggcga gctcctttcc cgtgcccacg tagtcgtcgt cgagatagac
     5281 cgccgcgtcg gaaggctcga tctcgaagcg cagccgggac cgctcggcgg gaagcgcctc
     5341 gcgaccagag cgcccctcct tcacctcgcc ccggaccttc ggcgccgggg cgaggtcctc
     5401 gtccgcctcg tccagctcct cggccagctc ctcgagctcc tcgaggtctt cgagctcctc
     5461 gaggtcacgc tcccggtcgg cctcggcccg tgcctcgaac cggccggtcc tgtcgcggcg
     5521 gggctcctcg ggcttgccga agacccgccc gtgaggcgtg ccgcgtcgcg gaggctcgac
     5581 cgcctcgagc ctcctcttgc ccggctcgag cttcaggtcg tcccggaaac ggaggctctg
     5641 tcccggctcg acctccaggt cgtgcttgac ggtctcgtac gagggatgcc ggaactcgag
     5701 cgtgtacttc ccctcctcga gccagaggaa gtcggggtag ccgtcgaagt cgtcggccga
     5761 gccgatgaac gtgccgtcga ggtagacctc cgccgcctcc gggctgacgt cggtgtcgac
     5821 ccgcgcgtac cggtacgggt ccgggctgta gtcctcgacg atgacgacgc cgccgaagcc
     5881 ccagtggtac gggccgaagc ggcttcccca ccaccaccag tcccacgggc caccccacca
     5941 ccacgacgtc ccgccccagc cggcgtacgg cgagtagtag cggcggcgcg gatggtgatg
     6001 cccgcccgag ccggagccgc gcggatgcgt cggaacgatt cgcgggaccc ggctgccccc
     6061 gccgcctgcc gtcgcggtcg tcgggccgcc gctccgcccg tcccgcggct cggcgccgac
     6121 gacgcgcctt cccgggcgcg ccggcgtccc cgaggtcgat tccctcgggg cggtcgcacg
     6181 gcggggcgtg gagggtgacc cggccgaggg cgccggagcg cttcgcggcg ccgaaggggc
     6241 gggcgcggaa taggacggcg acggagagga gtgggagggc gagggagcgg agtaggacgg
     6301 cgccggcgcc gagtaggagg gcgccggagc ggaccccgtg tccggcgagg ccgtccccgg
     6361 ctcgcgctgc gcgaccgccg aggtcgcgac gaggagggcc ggcaacgcga ggagcgcgcc
     6421 ggcgaggccc gggcgcgacc aggtcttcgc ccgggaggac gtgctggaga gtctcggatg
     6481 ggtcatggcg gcctccgctg cggcccgtcc tccccgaggg accggtgggc cgc
//
LOCUS       DAOONL010000167         6532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_14074_length_6532_cov_14.449591, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000167 DAOONL010000000
VERSION     DAOONL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14074_length_6532_cov_14.449591"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLP50_15655"
     CDS             complement(<1..434)
                     /locus_tag="PLP50_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53028.1"
                     /translation="MPRVCSVCSRPDRDAIDAAVAENRGSIRGIARQHGVSPDALERH
                     AKSHIPRTLARAAETREDARAEDLAAKTRRLEEDARRLLAKAEKEGDYRCAIAAVKTL
                     LDVVTLLHKVAEERKVEAQDLLKSSVSVFTTYGPPDAGFFTT"
     gene            complement(434..664)
                     /locus_tag="PLP50_15660"
     CDS             complement(434..664)
                     /locus_tag="PLP50_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53029.1"
                     /translation="MIDATKIPDAVFERLLLKTPPEALAAEVAELLAKRDHRLALVLV
                     LALLAQHEGLPASLPALLRATAGAEALLRGEA"
     gene            complement(774..1277)
                     /locus_tag="PLP50_15665"
     CDS             complement(774..1277)
                     /locus_tag="PLP50_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53030.1"
                     /translation="MTDRRDSLARRRDAAGLEAEELRRSLDAAAAAGEETSGIAEELV
                     ATEGRSKALALELAAVERALRSAEAVVGEERAAAERARLERAHAKAEAAFEDADRRFR
                     EALAALSASIGPLFKAEKERDRAAAAVGRPAPVFRPDRLDEELRDDLRRAGADTEVLA
                     LEVPFAT"
     gene            complement(1345..1608)
                     /locus_tag="PLP50_15670"
     CDS             complement(1345..1608)
                     /locus_tag="PLP50_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53031.1"
                     /translation="MNRTVVLTHREFAEAAEEIATRTGRSVGDVAAGLAGNVVVKEAE
                     ASLEHVDVRGYAHALAQFTAEHGCAVSAALAHVRPEDFARGVR"
     gene            complement(1657..1965)
                     /locus_tag="PLP50_15675"
     CDS             complement(1657..1965)
                     /locus_tag="PLP50_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53032.1"
                     /translation="MIRRLLERLRARLRPATVAPSAPAPVASETPSSPARLRLVPYPG
                     RRDDPAGVVLIRGGEVLVLDYAEVALLAAELPRLLDSLARFSPHYQPAEDAPPAAPIN
                     "
     gene            complement(2580..3596)
                     /locus_tag="PLP50_15680"
     CDS             complement(2580..3596)
                     /locus_tag="PLP50_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA53033.1"
                     /translation="MSGKPIPFREAASLLDDEVRAGLAELEGRVTDPGAFAPVTAEDL
                     LRRAFPPRELLLSPFLTKKSLTMVYARRGAGKTWLAEAAALSVAAAGTLFEARPNGPA
                     WAAPSPRSVLLVDGEMPGSMIRDRLALLIAGRLYEPARRLRVLAADLFEEPLPSISTP
                     EGRAVVEAHLGDAELVIFDNISTLFRGLDENDAGEWEPVQDFLLALRRRGLAVLLVHH
                     GGKNGAQRGTSKREDVLDTVVSLRLPDDHDPDEGARVHVVFEKARGLAGRDVESFEAR
                     LEVTKTGALWRAASIVDPDDEEILKLHRAGKSQREIARKVGRDQANVSRRLRKLVPRA
                     GGDA"
     gene            complement(3593..3856)
                     /locus_tag="PLP50_15685"
     CDS             complement(3593..3856)
                     /locus_tag="PLP50_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53034.1"
                     /translation="MTPEATALLAFDVDEALAGARGRALPPAEVLAAARRRNRARAFL
                     ALQDAARRGVQSEDPATEALLGRFRRDALREAGVDERLVRRTA"
     gene            complement(4022..4234)
                     /locus_tag="PLP50_15690"
     CDS             complement(4022..4234)
                     /locus_tag="PLP50_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPA53035.1"
                     /translation="MLEPVITNRELAARYGWKPQTERANRIKGQTPPYVKVGNRILYR
                     VADVLAWESVRHYTSTADERVRRASA"
     gene            complement(5120..6325)
                     /locus_tag="PLP50_15695"
     CDS             complement(5120..6325)
                     /locus_tag="PLP50_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPA53036.1"
                     /translation="MRRGDRNLTTKTVAALKKPGWYCDRDIPGFVVRVLPSGTKAFAV
                     RYVVKGTGIRRFTSLGLFGVVTVDQARRKAREILSAVALGDDPLGKRCPTWGEWSVTY
                     FDRLTAKSKNVFHRALLGFTEATNKRSGEPTDSTFREIRLRWAPRLLSSFRPEDIERE
                     RAAIRAAKSATVANRWLAVVSGVFGAAVRAELLGRNPVKSVRSDREAPARSRTLSTEE
                     MKRLLAALEGEKERDPWAAVGVLLAVLTGARRGEVLRLRWEDVRLDEGFARLPDSKSG
                     KPRFLALPARAVEELRELPRVGSYVVAPARDPLPGKDEKPRADLKAAWERLTTKATLE
                     GITFHDLRRTYGRELNRAAGLRVAQEALGHSTPEQTAAAYTPEGFDAIREATEKRATL
                     LPFPKRAAG"
BASE COUNT          911 a         2209 c         2427 g          985 t
ORIGIN      
        1 aaggtcgtga aaaacccggc gtcagggggt ccataggtcg tgaaaaccga cactgacgac
       61 ttgaggaggt cctgcgcctc caccttccgt tcctcggcga ccttgtggag gagcgtcacg
      121 acgtcgagga gcgtcttcac ggccgcgatg gcgcacctgt agtccccttc cttctctgcc
      181 ttcgccagga gccggcgggc gtcctcctcg agccggcgcg tctttgccgc gaggtcctct
      241 gcgcgagcgt cctcccgggt ctccgcggcg cgcgcgaggg tacgggggat gtggctctta
      301 gcgtggcgct cgagggcgtc gggggaaacg ccgtgctgcc gcgctatgcc gcgaatagag
      361 ccgcggttct ctgcgacggc cgcatcgatc gcgtcccggt cgggacgaga gcagacggag
      421 caaacccgcg gcatcaagcc tctccgcgga ggagcgcctc ggcccccgcg gtggcccgga
      481 gcagggccgg gagggaggcc gggagccctt cgtgctgtgc caggagcgcg aggacgagga
      541 cgagcgcgag gcggtgatcc ctcttcgcga ggagttcagc gacctcggcc gccagcgcct
      601 ccggcggggt cttgagaagt agccgctcga agacggcgtc cgggatcttg gttgcatcga
      661 tcatcggccg gtctccttcg tgggaaagac cccgccccgc cgtcacccgc ccgatccggc
      721 gcgcccgaag gaggcgcaga agggcgacga ggggcgggaa gtctggacgc ctgttaggtc
      781 gcgaacggga cttcgagcgc gaggacctcg gtatcggccc ccgctctccg gagatcgtct
      841 cggagttcct cgtcgagccg atcggggcgg aacaccggcg cgggccggcc cacggctgcc
      901 gcggcgcggt cgcgttcctt ctccgccttg aacagcggac cgatcgaggc gctgagagcg
      961 gcgagagctt ctcggaagcg ccgatcggcg tcctcgaaag cggcctcggc cttcgcgtga
     1021 gctcgctcga ggcgcgccct ctcggccgcg gcccgctcct ctccgacgac ggcctcggcc
     1081 gagcggagcg ctcgctcaac cgcggcgagc tcgagcgcga gcgctttcga tcgtccttcg
     1141 gtggcgacga gctcctcagc gatcccggag gtctcctccc cggccgcggc ggcggcatcc
     1201 aggctccggc gcagctcctc tgcctcgagc cctgcggcgt ctcggcggcg ggcgagcgag
     1261 tctcgccggt cggtcaaggc ggcgaccttc tcggcggcgg gcgaggccgg gctctcggcc
     1321 gtggcggcgg gggacggcga gcgcctaccg aacgccacgg gcgaagtcct ccgggcggac
     1381 gtgcgcgagc gcggcggaga cggcgcaacc gtgctcggcc gtgaactgcg cgagagcatg
     1441 cgcgtaaccg cggacgtcga cgtgctcgag gctggcctcc gcttccttga cgacgacgtt
     1501 cccggccaag ccggcggcga catcgccgac ggagcggccg gtccgcgtcg cgatctcctc
     1561 ggcggcttcg gcgaattcgc ggtgagtcag gacgacggtt cgattcatgg gcgttccctt
     1621 tcgttcggtc ggtttccgat gggcgcggcg gcgcggctag ttgatcggtg cggccggcgg
     1681 agcgtcctcg gccggctggt agtggggcga gaagcgggcg agggagtcga ggaggcgcgg
     1741 aagctcggcc gcaagaaggg cgacctcggc gtagtccagg acgaggacct cgcctccgcg
     1801 aatcaggacg actccggccg ggtcatctcg ccggccgggg tacggaacta gccggaggcg
     1861 cgccggcgac gatggggtct cggaggcaac cggcgccggt gcggatggcg cgaccgtggc
     1921 gggccgaagg cgggcgcgga gccgctcgag gaggcgccga atcacgctgc gcctcgcggg
     1981 tagacgcctc cccggccggc cccaggagcc ccggggctga cgggtcgccc ggcaaggggc
     2041 cgggccgggg tggacgagga atcagggaag catcggccgc acgtcgcgtc gccccgggcg
     2101 ttgacccgcc aggcgacggc cccgcacgcc gcgcacgggc ggccggcgag gcgcgcgagg
     2161 gtccggctgc gccggtcggg gtcgaagcgg aggaccctgc cgggacctcg ccgcgcgctg
     2221 tagcaggtgt cgcagaggag gtcctccgag cgggcgacgg gggagccgca cgcggggcag
     2281 ggaggggcgg gcgaggcggc ttccccgccg atgacgcgga gggcgagggt cacggcgtcc
     2341 gatgcggggt gatgcggggt gatgcggggt gatgcgttcc cgagcaggat caaacgcatc
     2401 actgatgcgc tgatgcgcgc ctataggggc cgcatcgcgc atcacccgag ggtccgggga
     2461 ggcgaaaccc gcatggttgt gcgtcggaac ggtgatgcgg ggtgatgcgt tcggggcctt
     2521 tccgaaaagg ggtccgcgca tcacccctga tgcgcgagtg atgcgctccc gcgaggcgct
     2581 cacgcatcac ccccggcacg cggaacgagc ttccggaggc gccttgagac gttcgcttgg
     2641 tcgcggccga ccttccgggc gatctcccgc tgactcttcc ccgcccgatg cagcttgagg
     2701 atctcctcgt cgtccgggtc gacgatcgac gcggcccgcc agagcgcccc cgtcttcgtc
     2761 acctcgagcc gagcctcgaa agactcgacg tcccggccgg cgagcccccg ggccttctcg
     2821 aagacgacgt gaacgcgggc gccttcgtcg gggtcgtgat cgtcgggaag gcggagcgag
     2881 acgacggtat cgaggacgtc ctcgcgcttc gaggttccac gctgcgcccc gttcttcccg
     2941 ccgtgatgca cgaggaggac cgcgaggccg cgacgacgga gggccaagag gaagtcctgg
     3001 acgggctccc attcgcccgc gtcgttctcg tcgaggccgc ggaacagcgt cgagatgttg
     3061 tcgaagatga cgagctcggc gtccccgaga tgagcctcga ctacggcgcg cccctcgggg
     3121 gtggagatcg acgggagggg ctcctcgaag aggtccgcgg cgaggacgcg gaggcgtctg
     3181 gccggttcgt agaggcggcc ggcgatgagg agcgcgagcc ggtcgcggat catcgaccca
     3241 ggcatctccc cgtcgacgag gaggacagat cgcggcgatg gcgcggccca tgcgggcccg
     3301 ttcgggcgag cttcgaagag ggtcccggcg gctgcgacag agagggcggc ggcctccgcg
     3361 agccaggtct ttccggcccc gcggcgcgcg tagaccatcg tcaagctctt cttcgtgagg
     3421 aacggggaca ggaggagctc gcgaggcggg aacgcgcggc gaaggaggtc ctcggcggtg
     3481 acgggggcga aggcgcccgg gtcggtcacg cggccctcga gctcggcgag gccggccctc
     3541 acctcgtcgt cgaggagaga ggccgcctcg cggaagggaa tgggcttccc gctcacgcgg
     3601 tcctccggac gagccgctcg tccacgccgg cctcccggag agcgtcgcga cgaaaccggc
     3661 cgaggagggc ctccgtcgcg gggtcctccg actggacgcc gcggcgggcg gcatcctgaa
     3721 gggccaggaa ggcccgagcc cggttccgcc tccgggcggc ggcgaggacc tcggccggcg
     3781 ggagcgcccg gccccgggcc cccgcgaggg cctcgtccac gtcgaacgcg aggagggcgg
     3841 tcgcctcggg cgtcacgacg cgacccccat cgcacggagg agcgcgaggc gcctccggtc
     3901 gcgtccgggg ttacgatcgg ggtggcgtag aattcgctgg tcgcaagcct tggattcgcg
     3961 cttgagccgt tcgggaattc cacctcccgg acggctcgct tcgtctctgt tcatggcggt
     4021 ttcaggcgct cgcgcggcgg acccgctcgt cggcggtcga cgtgtagtga cggacgctct
     4081 cccacgccag gacgtcggcg acccggtaca ggattcggtt tccgaccttc acgtagggcg
     4141 gggtctgccc cttgattcgg ttcgcgcgct ccgtctgggg cttccacccg taccgggcgg
     4201 cgagctcgcg gttcgtgatg acgggttcca gcatcgattt ctcctttcgg acgtttgctc
     4261 gcgcgttccc gcgtgtcttc tacgatcccg aacgtaggcc ctcccgaaac ctcacggggc
     4321 ggcttcttat tcgcctttcc tgacgctctc cgtgcgttct gaatcaggtt tccgagctcc
     4381 ttctctggcg tgcggtcctt ctcgtcgaag gtcaccgtcg ggtcgatctt gacggcgtag
     4441 gtttcgcccg ccgtcccgcg cgttcgagac catgcccacg cgacgaggcg ccgttcatcc
     4501 gacgtttcgg agaggaggct ccggcgtgta cgcgcgtgcg caagggcaac cgcaagttcg
     4561 gggcggggag cgaagtcgct gcacgcggcg agaatctcgg ccgcgccctt gagtcccttc
     4621 gtcgtccgtc gcatccgacg gaagtccgaa acgaggacat cgaagtgggc gaggtccgcg
     4681 cgtcggcgct cgcggtcggg cagcggaacg gggcctctcc cgcgcttccc gcgcccgggc
     4741 tcgatcgtcg cgttcggaga aaggatcttc gccacctcgt cgaaagcctc cctctcgcct
     4801 tccgcacgtc tcgagccgag cagagagagg agcgcggcga cggtcgggga atagaagagc
     4861 ggaaggcgcc cgaggagagc ggtcaggagg cccgggagcg cgtccgtcaa gcgcccgaac
     4921 ggcatccctc caggccgcgc gagatgggcg tctaccgcct cctgccccgc agccgcgagc
     4981 cgtagcactc ccgcgaccga ggcgaacact tcgcgctcga ggagggcagc cgcggcacgt
     5041 agctccgaag gcgtcgggag cccgctctct tgccgtgacg gggtgcgggg tctctgttcc
     5101 atcgcctctc ccttcgttct cagccggcgg cccttttcgg gaacggaagg agcgtcgcgc
     5161 gcttctcggt cgcctcgcgg atggcatcga agccctcggg tgtgtacgcg gcggccgtct
     5221 gttctggcgt cgagtgcccg agggcctcct gagcgacgcg gagaccggcg gcacggttca
     5281 gttcccggcc gtaggttcgc ctcaagtcgt gaaacgtgat cccctcgagc gtcgccttcg
     5341 tcgtgaggcg ctcccaggca gctttcaggt ccgcgcgcgg cttctcgtcc tttccgggca
     5401 gcgggtcacg ggccggggcg acgacgtagg agccgacgcg cgggagctcc cgtagctctt
     5461 ctacggcgcg ggccggcagg gccaggaacc ggggcttgcc gctcttcgag tcggggaggc
     5521 gggcgaaccc ttcgtcgagc cgaacgtcct cccagcgaag ccggaggacc tcgccccttc
     5581 tggcgcccgt caggacggcc aggaggacgc cgacggcggc ccaggggtcc cgttccttct
     5641 cgccctcgag cgcggccagg aggcgcttca tctcctccgt ggagagagtc ctcgagcggg
     5701 ccggcgcttc gcggtcggat ctcacgctct tgacggggtt ccggccgagg agctcggcgc
     5761 gaacggccgc gccgaagacg ccggagacga cggcgagcca tcggttcgcg acagtcgcgg
     5821 acttcgccgc gcgaatcgcg gcgcgctccc tttcgatgtc ctcgggtcgg aacgaggaga
     5881 ggaggcgagg agcccagcgg agacggatct cccggaaggt cgagtccgtc ggctcgcccg
     5941 accgcttgtt cgtcgcctcc gtgaacccga ggagtgcccg gtgaaagacg ttcttcgact
     6001 tcgcggtgag gcggtcgaag taggtcacgg accattcccc ccaggtcggg cagcgcttcc
     6061 cgagcgggtc gtctcccagg gcgacggcgg agaggatctc ccgcgccttc cgcctcgctt
     6121 ggtcgaccgt gacgacgccg aagaggccga gcgacgtgaa acggcgaatg cccgtcccct
     6181 tgacgacgta gcgcacggcg aacgccttcg taccggacgg gagaacgcgg acgacgaaac
     6241 ccgggatgtc gcgatcgcag taccacccgg gctttttcag ggcggccacc gtcttcgtcg
     6301 tgaggttccg gtctccccgt ctcatcgttg cctcccgcga gcgcgctcgg aagcggttgg
     6361 ggtgccgtgt gcctttccgc ttccgggtca gaatctacgc tctttcgcgt gcgagtcaac
     6421 gaagaaccgc ttggaaagcg gacttgatca acgggtcgga atcgctcggc ggaggcgacc
     6481 ctcagactcc ggatctaaag gtcccgcgtt cgaatcgcgg cgggcgcacc ac
//
LOCUS       DAOONL010000168         6296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_14752_length_6296_cov_17.609998, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000168 DAOONL010000000
VERSION     DAOONL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_6296_cov_17.609998"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLP50_15700"
     CDS             <1..767
                     /locus_tag="PLP50_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPA53037.1"
                     /translation="VALLQLDLAWEDVPANHARASRLLGQAADLGARLAILPEMFATG
                     FSMDGRNVAQAEEGATATFLRERAASLGLHVLAGVAELAEPLPANRALLVSPDGSVRR
                     YTKIHPFSFAGEEKVMASGDAVVTWDVEGLRVTPLVCYDLRFPEPFRLAADDTDLYAV
                     IANWPERRRHHWSLLLRARAVENLAFVAGVNRVGDGDDLRYLGDSALVGPWGETVVSA
                     AEQETLLVADVDPAAVAEARRRFPALADRRPGSYRR"
     gene            complement(755..2374)
                     /locus_tag="PLP50_15705"
     CDS             complement(755..2374)
                     /locus_tag="PLP50_15705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA53038.1"
                     /translation="MTLPEGPPASQPQGRDATRRLASSALLIAVLGIGTILRLSEGIS
                     GGVWLDEYHTLLLAGAPTISEVLARTSVEAHPPAYLIALHLVMRLVGEDPLVAKGLSV
                     LCGVATLLLVALAAHRRRGVLSAIVASTVLGGSAVAVHFATEIRPYAVVALTILVGLL
                     ATVSAVERPTLWRLLAQAGALIVALGLNFLALVLLPVGPLLGLLRAKPRLVAKQLLIG
                     AVAVLVTSPPLVRVATELPPEANEYLQTFWQGRTAVDALVVVGRDLLPSGRWPPAYGS
                     TARRTGFDQGLEAFAMAGVVLVLAGAGAAGFARGGSRRLDLLDAGCAALLASAAALAS
                     ASFLLGRPVVTPGRFAASFLPVVALLAGTAAAWGSVGRVGSLLLAAVAAANAGATLVG
                     SEQDGVRIDRNELVSVVAARELRGPLLVISVGLTGTPLKYELRTRRDVSFASYPVEVD
                     SHFGWWAPQKALSEPGALRRDAAMLTGRAVAAAREGRIVLLTGGDHPVAAPLHAALSQ
                     AFLARPLHPLARGASVLVLREAVPGPASQRR"
     gene            complement(2371..4377)
                     /locus_tag="PLP50_15710"
     CDS             complement(2371..4377)
                     /locus_tag="PLP50_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53039.1"
                     /translation="MSHPGRAVRVTLAVGALLPAALLVWSVLGMHRGLLLSDGLRGHF
                     WPWAPELGRPSGLSPSASALSDPVWQFVPWVEMAKRELGAGRLPLWNPHQEAGQPLLA
                     NAQSALGSPLLWPALALPLFPGWNLSLLLRLLASAVGGYLLARDLGRSRAASLLAASV
                     CGLSGPFVAWLEHPHTLTAGAVPWLLLAVRRAGRVARPWASAGVAATTALVLAGGHPE
                     TAMMAALLAAAFLVAGGQDRRAVAPALAGAVLGAGLAAPLLLPFLEYLLQSAAWIGAD
                     RYAAPLPLRDLLRLLTPSLRGSHPIEAAAYLSVGVLPLAVAGAFTGRRERDSRMLIVA
                     SASLLVAAYANPLSDLLASASTVRWSRTLLLLPIPVSLLAARGLDGLSTRARRAGRAA
                     PHLAFGIAAGCAAELLAAARGVHAVAPENPLLRTPILERLAADPGPFRILPLHTFLPA
                     NTATSLGLDDLRGYDALAVRAFRTGREKAGRFRGVPTHTDVVAPWDLARGGRELDAWN
                     VKYLLLHPQFAFSAATMNERLGLDLEEVYSGADGKLLLNRRAKSRARVERNGRDEGAV
                     VIVDRTPTRWLFDVTAPAGGTLVVANAGYPGWRAYVDGRPARVGGGVGRRQEIDIASG
                     RHRVDLAYRPLSFLLGLLVSAVTVLILGAGLARPHRSMGSRTGR"
     gene            complement(4386..4865)
                     /locus_tag="PLP50_15715"
     CDS             complement(4386..4865)
                     /locus_tag="PLP50_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPA53040.1"
                     /translation="MSGRESAVETRLSSILARGRVETGFSALSPEDAVERLLRPVLLA
                     EGFSEDAADAALEGIRNREGAGSTSFGAVALPHARVPRLGRIVGALGVNGGGVYASGP
                     QGIRVVLAFASPASATVEHLQFLAQVARLLRQEETVADLLEARDGDALLAAIRRRER"
     gene            4952..>6296
                     /locus_tag="PLP50_15720"
     CDS             4952..>6296
                     /locus_tag="PLP50_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPA53041.1"
                     /translation="MSLTLLDWGIVFAYFALSIGVGLAFTKKGGESLEEYFVAGRSAP
                     WWLAGSAMIATTFAADTPLVVTGLVARHGVAGNWLWWNMVLSGTLTVFLYARLWRRAN
                     VLTDVEFAEVRYSGRPATFLRGFRAVYLALPINLIIMGWVTLAMVKILGIALGIPPVW
                     AVGICFVITVAYSAAAGIRAVLWTDLVQLVIMMTAVIALAVYAVRAVGGMGALETGLA
                     SHFGSLDAALSVVPPAGSPWMPAIALFTFLAVQWWAAWYPGAEPGGGGYVAQRIFSAR
                     SEKDGILATLFFNVGHYAFRPWPWIVTGLCTVLLYPGGVQVGGKADVEAAYVQAMVDH
                     LPAGWRGFLLAGFAAAYMSTMATHLNWGASYLVNDVYRRFLKPMASERHYVLAGRLTT
                     ILLFLASIVVTLNLGTIEGAWRFLLALGSGTGLVLILRWYWWRINAWSEISAMGAS"
BASE COUNT          944 a         2271 c         2304 g          777 t
ORIGIN      
        1 aggtcgccct cctccagctc gacctcgcct gggaggacgt cccggcgaac cacgcgcgcg
       61 cctcccgcct cctcggccag gccgccgacc tcggcgcccg cctcgcgatc ctcccggaga
      121 tgttcgcgac cggcttctcg atggacggcc ggaacgtcgc gcaggccgag gagggagcga
      181 ccgcgacgtt cctccgcgag cgggcggcct ccctcggcct gcacgtcctc gccggcgtcg
      241 ccgagctcgc cgagccgctc cccgcgaacc gcgccctcct cgtctccccg gacggctccg
      301 tccgccgcta cacgaagatc caccccttct cgttcgcggg cgaggagaag gtcatggcga
      361 gcggcgacgc cgtcgtcacc tgggacgtcg aagggctgcg cgtgacgcct ctcgtctgct
      421 acgacctccg cttccccgag ccgttccgcc tcgcggcgga cgacaccgac ctgtacgccg
      481 tcatcgcgaa ctggccggag aggcgccgcc atcactggag cctcctcctg cgcgcccgcg
      541 ccgtggagaa cctcgccttc gtcgcgggcg tcaaccgggt cggcgacggg gacgacctcc
      601 gctacctcgg cgattccgcc ctcgtcggcc cctggggaga gacggtcgtc tccgccgccg
      661 agcaggagac gctcctcgtc gcggacgtcg atccggcggc ggtcgccgag gcgcggcggc
      721 ggttccccgc gctcgcggac cggcggcccg gcagctaccg gcgctgagag gccggccccg
      781 gtacagcctc ccggaggacg aggaccgatg ctccccgggc gaggggatgc agcggccgcg
      841 cgaggaacgc ctggctcagc gcggcatgga ggggtgccgc cacgggatgg tctcccccgg
      901 tcaggaggac gatgcgtccc tcccgcgccg cggcgacggc gcggccggtc agcatcgccg
      961 cgtcacgccg gagcgctccg ggctcggaaa gagccttctg cggggcccac cagccgaaat
     1021 gggaatcgac ctcgaccgga tagctcgcga aggagacgtc acgacgcgtg cggagctcgt
     1081 acttcagggg agtccccgtc agtccgaccg agatgaccag gagcgggcca cggagctctc
     1141 gcgcggcgac cacgctgacc agctcgttcc tgtcgatgcg gactccgtcc tgctccgacc
     1201 cgaccaacgt cgctcccgcg ttcgcggcgg cgacggcggc cagaagcaga gaccccacgc
     1261 gtccgacact tccccaggcg gctgccgtcc cggcgagcag ggccaccacg ggcaggaagg
     1321 aggccgcgaa ccgtcccggg gtgacgaccg gtcgccccag gagaaaggac gcggacgcga
     1381 gcgcggcagc agaggcgaga agcgccgcgc aacccgcgtc gagcaggtcg agccgccggc
     1441 tgccccccct ggcgaaaccg gccgcgccgg cgccggcaag gaccaggacg acaccggcca
     1501 tcgcgaacgc ctcgagcccc tgatcgaagc ccgtgcgccg cgccgtggat ccgtaggccg
     1561 gcggccagcg gccggacggc agcaggtccc gccccacgac gacgagcgca tcgaccgccg
     1621 tccgtccctg ccagaaggtc tggaggtact cgttcgcctc cgggggaagc tccgtcgcga
     1681 cgcgcacgag aggtggcgag gtcacgagga cggccacggc accgatcaac agctgcttcg
     1741 cgacgaggcg cggcttcgca cgaaggagac cgagcagcgg cccgaccggc aggaggacga
     1801 gggccaggaa gttcaatccg agagcgacga tcagggcccc ggcctgggcg agcagcctcc
     1861 agagcgtcgg gcgctcgacc gctgagaccg tggccaggag ccccacgagg atcgttaggg
     1921 cgacgaccgc gtaggggcgg atctccgtgg cgaagtggac ggcgacggcc gacccaccca
     1981 ggacggtcga cgcgacgatc gcagaaagaa ccccccgacg tcggtgcgct gcgagcgcga
     2041 cgaggaggag cgtggcgact ccgcagagga ccgagagccc tttcgcgacg aggggatcct
     2101 ccccgacgag ccgcatgacg aggtggagcg cgatcaggta ggcgggtgga tgcgcctcca
     2161 cggacgtccg agcgagcact tccgagatcg tgggcgctcc cgcgagaagg agcgtgtggt
     2221 actcgtcgag ccagacccct ccgctgatcc cttcggagag ccgcagaatc gtaccgatcc
     2281 ccagcacggc gatcaggagc gccgaggacg cgagccgtcg agtcgcatcg cggccctgcg
     2341 gctgactggc gggaggaccc tcgggtagcg tcatcgaccc gtccgtgatc ccatcgaacg
     2401 gtgcggcctc gccaggccgg caccgaggat gaggacggtc accgcagaca ccaggaggcc
     2461 gaggaggaac gagagcggac gataggcgag gtcgacgcgg tgccgccccg aggcgatgtc
     2521 gatctcctgc cttcgaccga cgcctccgcc gacccgtgcg ggacgcccat cgacgtaggc
     2581 tctccagccc ggatagccgg cgttggcgac gacgagcgtc ccgcccgccg gcgccgtcac
     2641 gtcgaagagc caccgtgtcg gcgtcctgtc gacgatcacg acggctccct catcccgccc
     2701 gttgcgctcg acccgggccc gcgacttcgc cctccggttc aggaggagct ttccgtccgc
     2761 gcccgagtag acctcctcga ggtcgagacc gaggcgctcg ttcatcgtcg cggcggagaa
     2821 cgcgaactgg ggatggagca ggaggtactt cacgttccag gcgtcgagct cgcgcccgcc
     2881 cctcgcgagg tcccagggag ccacgacgtc ggtgtgcgtc gggacgccgc gaaagcgccc
     2941 cgccttctcc cgcccggtcc ggaaggcccg caccgcgagg gcgtcgtagc cgcgaaggtc
     3001 gtcgagcccg agggacgtgg ccgtgttcgc cggcaggaac gtgtgaagcg ggaggatccg
     3061 gaaggggccc gggtcggcgg cgaggcgctc cagaatcggc gtccggagca gcggattctc
     3121 cggcgcgacg gcgtggactc cgcgcgcggc cgcgaggagc tcggccgcac atccggccgc
     3181 gatcccgaaa gcgagatggg gtgccgcccg acccgcgcgc cgcgcccgcg tcgagaggcc
     3241 gtcgaggccg cgcgcggcga ggagggagac ggggatcggc aggaggagga gcgtccgact
     3301 ccagcggacc gtcgaggccg atgcgaggag gtcagagagc gggttcgcgt aggccgcgac
     3361 gaggaggctg gcgctcgcga cgatgagcat ccgggagtcg cgctccctcc gccccgtgaa
     3421 cgcgcctgcg accgcgagag ggaggactcc gacggagagg tacgcggccg cctcgatcgg
     3481 atgcgaaccg cgaagggacg gggtgaggag ccggagcagg tcccgcagcg ggagaggagc
     3541 ggcgtatcgg tcggctccga tccatgccgc gctctggagg aggtattcca ggaacgggag
     3601 caggagaggc gcggcgagcc ccgctccgag gacggccccg gcgagcgccg gcgcgacggc
     3661 gcgacggtcc tgccccccgg cgacgaggaa ggcggccgcg aggagcgctg ccatcatcgc
     3721 cgtctccggg tgcccgccgg cgaggacgag agccgtcgta gctgccacgc cggccgacgc
     3781 ccatggccgc gccacgcgtc cggcgcgtcg gacggcgagg agaagccacg ggacggctcc
     3841 cgccgtcagc gtgtggggat gctcgagcca ggcgacgaac ggccccgaca acccgcagac
     3901 cgaggccgcc agcagggatg cagctcggga acggcccagg tcgcgcgcga ggaggtagcc
     3961 gcccacggcc gatgccagga gccggaggag gagagagagg ttccagcccg ggaagagcgg
     4021 cagggccagc gcgggccaga ggagaggcga gccgagggcc gactgggcgt tcgcgaggag
     4081 cggctggccg gcctcctggt gcgggttcca gaggggaagg cgtccggcgc cgagctcccg
     4141 cttcgccatc tcgacccacg gcacgaactg ccagaccggg tcggagagtg ccgacgcaga
     4201 cggggagagg cccgacgggc gaccgagctc cggggcccag ggccagaagt gcccgcgcag
     4261 accgtccgaa agcagcaggc cgcggtgcat cccgaggacg ctccagacga ggagcgcggc
     4321 cggcagcaac gcgccgacgg cgagcgtgac ccggacggcg cggcccgggt ggctcacccg
     4381 ccccctcagc gctcccgccg ccggatcgcc gccaggagcg cgtcgccgtc ccgcgcttcg
     4441 aggaggtcgg ccaccgtctc ctcctggcga aggagccggg cgacctgcgc gaggaactgg
     4501 aggtgctcga cggtcgcgga tgcgggagag gcgaaggcga ggacgacccg gattccctgc
     4561 gggccggagg cgtagacgcc gccgccgttc acgccgagcg cgccgacgat ccggccgagg
     4621 cgggggaccc gcgcgtgcgg gagagccacg gcgccgaacg acgtcgaccc ggctccctca
     4681 cgattccgga ttccctcgag cgccgcgtcc gcggcgtcct cggagaagcc ctccgcgagg
     4741 aggacgggcc ggaggaggcg ctcgacggcg tcctcggggc tcagcgccga gaagccggtc
     4801 tcgactcggc cgcgggcgag gatcgaggag agccgcgtct cgacggccga ctcgcgcccg
     4861 gacacgcagc gtcctccacg ccgcgcatga tcgtgccgcg ccgcggcggt ggcaagggaa
     4921 aagtcccgga ccgctaagat cgacgaccga catgagcctg acgcttctgg actggggaat
     4981 cgtcttcgcc tacttcgcgc tgtcgatcgg ggtcggcctc gccttcacga agaagggggg
     5041 cgagtcgctc gaggagtact tcgtcgccgg gcgctccgcg ccgtggtggc tcgcgggctc
     5101 cgcgatgatc gcgacgacgt tcgcggccga cacgccgctc gtcgtgacgg gtctcgtcgc
     5161 ccggcacggc gtcgccggga actggctctg gtggaacatg gtcctgagcg ggacgctcac
     5221 cgtcttcctc tacgcgcgcc tctggcgacg cgcgaacgtc ctgaccgacg tcgagttcgc
     5281 cgaggtccgc tattccggcc gcccggccac gttcctccgc ggctttcggg ccgtctacct
     5341 cgcgctcccg atcaacctga tcatcatggg ctgggtcacg ctcgcgatgg tcaagatcct
     5401 cggcatcgcg ctcgggatcc cgcccgtctg ggccgtcggc atctgcttcg tcatcacggt
     5461 cgcctattcc gcggccgccg ggatccgcgc cgtcctctgg accgacctcg tccagctcgt
     5521 gatcatgatg acggccgtca tcgccctcgc cgtctacgcc gttcgcgccg tcggagggat
     5581 gggcgctctc gagacgggcc tcgcctccca cttcggctcg ctcgacgccg cgctttccgt
     5641 cgtcccgccc gccggctcgc cctggatgcc ggcgatcgcg ctcttcacgt tcctcgcggt
     5701 gcagtggtgg gccgcctggt atccgggcgc cgagccgggc ggcggcggtt acgtcgcgca
     5761 gcgcatcttc tcggcacgga gcgagaagga cgggatcctc gcgacgctct tcttcaacgt
     5821 cgggcactac gcgttccgcc cgtggccctg gatcgtgacc ggtctctgca ccgtcctcct
     5881 ctatccgggc ggcgtccagg tcggagggaa ggcggacgtg gaggcggcct acgtccaggc
     5941 gatggtcgac cacctcccgg ccggctggcg gggcttcctc ctcgccggct tcgcagccgc
     6001 ctacatgtcc acgatggcca cccacctgaa ctggggggca agctacctcg tcaacgacgt
     6061 ctatcgccgg ttcctgaagc cgatggcgag cgagcggcac tacgtgctcg ccggacggct
     6121 gacgacgatc ctcctcttcc tcgcctcgat cgtcgtgacg ttgaacctcg ggacgatcga
     6181 gggagcctgg aggttcctcc tcgcgctcgg gagcgggacg gggctcgtcc tgatcctccg
     6241 ctggtactgg tggcggatca acgcctggag cgagatctcg gcgatgggcg cctccg
//
LOCUS       DAOONL010000169         6121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15327_length_6121_cov_15.503792, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000169 DAOONL010000000
VERSION     DAOONL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15327_length_6121_cov_15.503792"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLP50_15725"
     CDS             <1..849
                     /locus_tag="PLP50_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA53042.1"
                     /translation="EVDWPAIRSWNVAQGQFFTDQDNRAAAKVCVLGQTVATTLFGSE
                     DPIGKAIRIKNIPFRVVGVLEPKGGSMMGQDQDDTVIAPYQTVRKKIMGTTAVGMIMA
                     AVSTPELVPQAQEEITALLRQRHRINRAAGQEDDFMIRSQTEMLARAEEQAKTMAILL
                     WSIAGVSLLVGGIGIMNIMLVSVTERTREIGVRMAIGAKGADIRAQFLVEAVVLAIAG
                     GVLGIALGVGIQQAVARLAGWPVAVTTSSVSLAFVFSAVIGVTFGFYPALKASRLDPI
                     EALRYE"
     gene            complement(969..1628)
                     /locus_tag="PLP50_15730"
     CDS             complement(969..1628)
                     /locus_tag="PLP50_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA53043.1"
                     /translation="MGLVLVETFPVAPLGCNCAIVADPQSKAAIVLDPGGEPDRVLAA
                     LARHGLSAVALVHTHAHLDHCLSSARLSKETGAPILMHEGDLPLYGDLARWGRMFGLT
                     PEAPGPVARTLGHGDRVACGGGALEVLHTPGHTPGSVCFRLDGERPLLFSGDTLFRRS
                     VGRTDFPGGSWDDLVSSIETRLLTLPGDLLVLPGHGEETTIGEESRLNPFLGDRARPL
                     A"
     gene            1682..2734
                     /locus_tag="PLP50_15735"
     CDS             1682..2734
                     /locus_tag="PLP50_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPA53044.1"
                     /translation="MSAVPHSDFRSDTVTKPTPAMRRAMSDAEVGDDVYGEDPTIARL
                     EARTAELLGFPAALFVPTGSMGNQIALRVHARPGTEVIVESRSHVFHYEMGAMAALSG
                     LLPRPVFGPGGRMAASEVEAWVRPESVYYLPRTSLVCLENTHNFAGGTVLPRAAVEEV
                     LALARRKGLAVHVDGARLWNAAAALGVPEASLVAGVDSVMVCFSKGLRAPVGSAVAGS
                     EAFVAEARRVRKLFGGGMRQAGVLAAAALVALEEERGRLAEDHARLARLARELAGIRG
                     VSVDPESFPTNILIADLEPRVFGSANDALGRLRERGILAGAAGPASVRLVAHADVGDA
                     DVDRCLAAFREIALGG"
     gene            complement(2763..3155)
                     /locus_tag="PLP50_15740"
     CDS             complement(2763..3155)
                     /locus_tag="PLP50_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPA53045.1"
                     /translation="MSQLKTIAVENAPKAIGPYAQGIVAGGFLFTAGQVHLDPATMKL
                     VSGTIAEETNQVFDNLQAVLAGAGCTLADVVKTTVFLTDMGTFAEMNGAYAARFGDHR
                     PARSTVQVGALPGNGRVEIELIAKLPGA"
     gene            complement(3152..3670)
                     /gene="hpt"
                     /locus_tag="PLP50_15745"
     CDS             complement(3152..3670)
                     /gene="hpt"
                     /locus_tag="PLP50_15745"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPA53046.1"
                     /translation="MIDRILISEQQIATRLDELAREIDAAYAGREILAVGVLKGSVFF
                     LTDLLKRLSTPVAVDFLQVRSYQGTSSTGNFELVRDLSSDIAGRDVVIFEDVVDTGLT
                     LRKLLGLLGTRGPRSVRIAALLKKDLPENADLPVDFLGFTIGPEFVVGYGLDLDEKYR
                     NLPYVAIWDPSK"
     gene            3712..5343
                     /locus_tag="PLP50_15750"
     CDS             3712..5343
                     /locus_tag="PLP50_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53047.1"
                     /translation="MSTSIVDLKTIHDAYTRLTEKFKTFWTFHQFLQGVHKTFFGDAP
                     GYNVDFQGLYDEIRAVTTVMNVEPPPVVIDRINQLDGRLDVVYRALSADDEKIAASWV
                     RRFFEKVRTEDEKLLLAILRFYFHARQVSPEALDKMDFLLTLVGARRSLDDGRYLPRF
                     PQELTKLFAALLGLVRRSPAPAAELTAAVKALGLLKKDIDSCERFEELTEKKILDSLR
                     TVKHRLGPAFYDSNVLSAILDANLAAKNRFQALYRSEEKRIFESSQQLLDIERELSRR
                     PEVADAELQEEFRRFRQFKDEFDKNRGEGEIRHAQVTRLAETIDQLMSKLDAAGEAPG
                     EQIPEILPLPDEELSEAQETVSGYFPGRIEGAAPPEPVKAARAKPAVATDPLLGEVAA
                     KILYSVDMLQVGAGSGQAAYGSSLSRLRLEPWEVRAARRVQSEDDRAPDAETAANDDL
                     FLEGAALRLRIDEAAQVLKAPSATAEDGKVLEMAAECLVRAQDLDRRFRQSLEAVGTE
                     GPPERLNELTRSRFRLLRAFSGLWLLHNARASAGE"
     gene            5404..>6121
                     /locus_tag="PLP50_15755"
     CDS             5404..>6121
                     /locus_tag="PLP50_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPA53048.1"
                     /translation="MPEDTPQQTEQSLAEQVGNRRAARSRIVDLGHPAYPNRFAATSL
                     VSDVVARWGGSDAAAIDAVPEEERRVAVPGRVLAIRKMGKAVFLDLSDGRQRVQAYVR
                     KDGVGDGGWALLENLDLGDHVGVAGVVFRTRTGELSVKAEALTFLAKCLRPLPDKWHG
                     LTDVERKYRQRYVDLIVSEETRRTFEIRARVVSYIRRFFDGRGFLEVETPMMQLIPGG
                     AAARPFVTHHNALDVDLYLRI"
BASE COUNT          923 a         2096 c         2221 g          881 t
ORIGIN      
        1 gaggtcgact ggcccgcgat ccgctcctgg aacgtcgcgc aggggcagtt cttcaccgac
       61 caggacaacc gggcggccgc gaaggtctgc gtcctcgggc agacggtcgc gacgaccctc
      121 ttcggcagcg aggatcccat agggaaggcg atccggatca agaacatccc gtttcgcgtc
      181 gtcggcgtcc tcgagccgaa gggaggctcg atgatgggtc aggaccagga cgacaccgtc
      241 atcgccccgt accagaccgt gcggaagaag atcatgggga cgaccgcggt ggggatgatc
      301 atggccgcgg tctcgacgcc cgagctcgtt ccgcaggccc aggaggagat cacggcgctc
      361 cttcggcaac gtcaccggat caaccgggcg gcaggccagg aggacgactt catgatccgc
      421 tcccagaccg agatgctcgc gcgcgccgag gagcaggcga agacgatggc catcctcctc
      481 tggtcgatcg cgggcgtttc gctcctcgtc ggcgggatcg ggatcatgaa catcatgctc
      541 gtctcggtca cggagcggac gagggagatc ggcgtgagga tggcgatcgg ggcgaagggg
      601 gccgacatcc gcgcgcagtt cctcgtcgag gcggtcgtcc tcgccatcgc cgggggcgtc
      661 ctcggaatcg cgctcggcgt tggcatccag caggccgtcg cgaggctcgc cggctggccc
      721 gtcgccgtca ccacgagctc cgtctccctc gccttcgtct tctccgccgt catcggcgtg
      781 accttcggct tctacccggc gctcaaggca agtcggctcg atccgatcga ggcgctccgg
      841 tacgaatgag ctgaagcctc cccccccaga cccttgcctc gcgtcccacc ccgcgccccg
      901 tcttcatcgc gcccgcgcga agcgcgcacc ggggagggcg gtgggtgggg ggcgcggggt
      961 gggggccttc aggccagggg ccgggcgcga tcgccgagga acgggttgag gcgagactcc
     1021 tctccgatcg tcgtctcctc gccgtgaccc gggaggacga ggaggtcgcc cgggagggtc
     1081 aggaggcggg tctcgatcga ggagacgagg tcgtcccagg atccgccggg aaagtccgtc
     1141 cggccgaccg agcgccggaa gagcgtgtcg cccgagaaga ggagagggcg ttccccgtcg
     1201 agccggaagc agacgctgcc gggcgtgtga cccggcgtgt ggagcacctc cagcgcccca
     1261 ccgccgcagg cgacgcggtc gccgtggccg agagtcctcg cgacgggacc gggggcctcg
     1321 ggagtcaggc cgaacatccg tccccagcgg gcgaggtcgc cgtagagcgg gaggtctccc
     1381 tcgtgcatca ggatcggcgc gcccgtctcc ttcgagagcc tcgcggagga gaggcaatgg
     1441 tcgaggtgcg cgtgcgtgtg gacgagcgcg acggccgaga gcccgtgacg cgcgagcgcg
     1501 gcgaggacgc ggtccggctc gccgcccggg tcgaggacga tcgccgcctt cgactggggg
     1561 tcggccacga tcgcgcagtt gcagccgagg ggggcgacgg ggaaggtctc cacgaggacg
     1621 aggcccatgg cgggcgagtc tgccacacgg cctccgcgcg gctcggccta cactcccgcc
     1681 gatgagcgcc gtcccgcact ccgacttccg ctccgatacc gtcacgaagc cgacccctgc
     1741 gatgcgccgc gcgatgtccg acgccgaggt cggggacgac gtctacggcg aagacccgac
     1801 gatcgcccgt ctcgaggcgc ggacggccga gctcctcggc ttcccggccg ccctcttcgt
     1861 tcccaccggc tcgatgggga accagatcgc gctccgcgtt cacgcccggc cggggacgga
     1921 ggtgatcgtc gagtcgcgca gccacgtctt ccactacgag atgggagcga tggcggctct
     1981 ttcggggctc ctcccgcggc cggtgttcgg gccaggcggg cggatggcgg cgtccgaggt
     2041 cgaggcctgg gtgaggccgg aatccgtcta ctacctcccg cggacctctc tcgtctgcct
     2101 cgagaacacc cacaacttcg ccggggggac ggtcctgccg cgagcggcgg tggaggaggt
     2161 cctcgccctc gcccgccgca aggggctcgc cgtccacgtg gacggggccc gcctctggaa
     2221 cgccgcggcc gcgctcggcg tcccggaggc ttcgctcgtg gcgggcgtcg actccgtgat
     2281 ggtctgtttc tccaaggggc tgagggctcc cgtcgggagc gccgtggccg gctcggaggc
     2341 gttcgtcgcc gaggcccgac gcgtccggaa gctcttcgga ggtgggatgc gtcaggccgg
     2401 ggtcctcgcc gccgcggctc tcgtcgcgct cgaggaggag cgagggcgcc tcgccgagga
     2461 ccacgcccgg ctggctcggc tcgcacgcga gctcgcgggc atccggggcg tctcggtcga
     2521 cccggagtcc ttcccgacga acatcctgat cgccgacctc gagccgcgtg tcttcgggtc
     2581 ggcgaacgac gcactcggac gcctgcggga acgcggcatc ctggccgggg cggccggccc
     2641 ggcctccgtc cgcctcgtgg cgcacgctga cgtcggggac gcggacgtcg accgctgcct
     2701 tgcggccttc cgggagatcg ctctcggggg ctgacgggca ggggcggctc ccgccccgct
     2761 cctcaggcgc cgggcagctt cgcgatcagc tcgatctcga cgcgcccgtt cccggggagg
     2821 gcgccgacct ggaccgtcga acgggccggg cggtggtcgc cgaagcgggc ggcgtaggcg
     2881 ccgttcatct cggcgaaggt ccccatgtcg gtcaggaaga cggtcgtctt cacgacgtcg
     2941 gcgagcgtgc agccggcgcc ggcgaggacc gcctggaggt tgtcgaacac ctggttcgtc
     3001 tcctcggcga tcgtccccga cacgagcttc atcgtggccg ggtcgaggtg gacctgcccg
     3061 gcggtgaaga ggaaccctcc ggcgacgatc ccctgggcgt aggggccgat ggccttcggg
     3121 gcgttctcga cggcgatggt cttcagctgg ctcatttgct cgggtcccag atggcgacgt
     3181 aggggaggtt gcggtacttc tcgtcgaggt cgaggccgta gccgacgacg aactcggggc
     3241 cgatcgtgaa gccgaggaag tcgaccggga ggtcggcgtt ctcggggagg tccttcttca
     3301 ggagcgccgc gatcctcacg gaacgcggac cccgggtccc gaggaggccg aggagcttgc
     3361 ggagcgtcag gcccgtgtcg acgacgtcct cgaagatcac gacgtcgcgt cccgcgatgt
     3421 ccgaggagag gtcgcggacg agctcgaagt tcccggtgga ggaggtcccc tggtacgacc
     3481 tcacctggag gaagtcgacg gcgaccgggg tcgaaagacg cttcaggagg tcggtgagga
     3541 agaagacgga gcccttcagg acgccgacgg cgaggatctc ccggcccgcg taggccgcgt
     3601 cgatctcccg ggccagctcg tccaggcggg tggcgatctg ctgctcggaa atgaggatgc
     3661 ggtcgatcac gggacgaaac tataatgcag acgcaactcc gagaggttca cgtgagcacg
     3721 tcgatcgtcg acctgaagac cattcacgac gcgtatacgc gcctgaccga gaagttcaag
     3781 accttctgga cgttccacca gttcctccag ggggtacaca agaccttctt cggggacgcc
     3841 ccgggctaca acgtggactt ccaggggctc tacgacgaga tccgggccgt caccacggtg
     3901 atgaacgtcg agccgcctcc ggtcgtcatc gaccggatca accagctcga cggtcggctc
     3961 gacgtcgtct atcgggccct ctccgcggac gacgagaaga tcgcggcgag ctgggtccgc
     4021 aggttcttcg aaaaggtccg gacggaagac gagaagctcc tcctcgcgat cctccgcttc
     4081 tacttccacg cgcgccaggt gtcgccggaa gcgctcgaca agatggactt cctgctgacc
     4141 ctggtcgggg cgcgccgctc gctcgatgac ggccgctacc tgccacggtt cccgcaggag
     4201 ctcacgaagc tcttcgccgc gctcctcggg ctcgtccgcc gcagccccgc gcccgcggcc
     4261 gagctgacgg cggcggtcaa ggccctcggc ctcctgaaga aggacatcga ctcgtgcgag
     4321 cgcttcgagg agctgaccga gaagaagatt ctcgacagcc tccggacggt gaagcaccgg
     4381 ctcggccccg ccttctacga ctcgaacgtc ctctcggcga tcctcgacgc gaacctcgcc
     4441 gcgaagaacc gctttcaggc cctctaccgg agcgaggaga agcggatctt cgagtcgtca
     4501 cagcagctcc tcgacatcga gcgggagctc tcgaggcggc cggaggtcgc cgacgccgag
     4561 ctccaggagg agttccggcg cttccggcag ttcaaggacg agttcgacaa gaaccggggc
     4621 gagggagaga tccggcacgc ccaggtcacc cgcctcgccg agacgatcga ccagctcatg
     4681 tcgaagctgg acgccgcggg ggaggcgccc ggcgagcaga tcccggagat cctgccgctg
     4741 ccggacgagg agctgtccga ggcgcaggag acggtgagcg ggtatttccc gggccggatc
     4801 gagggggccg ctccgccgga gccggtgaag gcggcccggg cgaagccggc cgtggcgacg
     4861 gacccgctgc tcggcgaggt cgcggcgaag atcctctact ccgtcgacat gctgcaggtc
     4921 ggggccggat cgggccaggc ggcctacggg agctcgctct cgaggctccg gctcgagccg
     4981 tgggaggtcc gcgcggcccg ccgggtccag tccgaggacg atcgcgcccc ggacgcggag
     5041 acggccgcga acgacgacct cttcctggag ggagccgcgc tccgcctccg gatcgacgag
     5101 gccgcccagg tcctcaaggc accatccgcg acggcggagg acgggaaggt cctcgagatg
     5161 gcggcggagt gcctggtgcg ggcgcaggac ctcgacaggc gcttccggca gagcctcgag
     5221 gcggtcggga cggagggtcc gcccgagcgc ctgaacgagc tcacgcgttc ccggttccgc
     5281 ctgcttcgag ccttctcggg cctctggctc cttcacaacg cgcgggcttc ggccggggag
     5341 tagccgccgg ccgacgggtg ctcgtttgac ccgcgcggga ggcgcgcgga gaatcggcgc
     5401 ccgatgcccg aagacactcc ccagcagacc gagcagagcc tcgccgagca ggtcgggaac
     5461 cgcagggcgg cccgcagccg gatcgtcgac ctcggccacc ccgcctatcc gaaccgcttc
     5521 gccgcgacca gcctcgtctc ggacgtcgtc gcccgctggg gtgggagcga cgccgcggcg
     5581 atcgacgcgg tgccggagga agagaggcga gtcgccgttc ccggccgcgt cctcgccatc
     5641 cggaagatgg ggaaggccgt cttcctcgac ctctccgacg gccggcaacg cgtgcaggcc
     5701 tacgtccgca aggacggcgt gggggacggc ggatgggccc tcctcgagaa cctcgacctc
     5761 ggcgaccacg tcggcgtggc cggcgtcgtc ttccggaccc gcacgggcga gctttccgtc
     5821 aaggcggagg ccctgacgtt cctcgcgaag tgccttcgac ccctccccga caagtggcac
     5881 ggcctgacgg acgtggagag gaagtaccgc cagcggtacg tcgacctgat cgtctcggag
     5941 gagacgcgcc ggacgttcga gattcgcgcg cgcgtcgtct cgtacatccg gaggttcttc
     6001 gacgggaggg ggttcctcga ggtcgagacc ccgatgatgc agctcattcc cggcggcgcg
     6061 gcggcgcggc cgttcgtgac gcaccacaac gcgctcgacg tcgacctcta cctgaggatc
     6121 g
//
LOCUS       DAOONL010000170         6101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15399_length_6101_cov_15.011413, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000170 DAOONL010000000
VERSION     DAOONL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15399_length_6101_cov_15.011413"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(638..1051)
                     /locus_tag="PLP50_15760"
     CDS             complement(638..1051)
                     /locus_tag="PLP50_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53049.1"
                     /translation="MTRVWEEAPSRVAVWLLGLFLLALTGAAFAESRPDERVTKDRKG
                     RVTSRETTIAGGARLRTRLEYDLDSLRPSLVIAEEHTASGRLTRRTEQRFDGEGRIRL
                     KTEIRIDETGKGTGTRTRYSYDASGRALEESAPAE"
     gene            complement(1132..4056)
                     /locus_tag="PLP50_15765"
     CDS             complement(1132..4056)
                     /locus_tag="PLP50_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HPA53050.1"
                     /translation="MKLAAHCAFLGLLLAACGAASAQETTLLQFNSEPDDWIGQGGQF
                     TLTPSDGQIVASRVDGGVVVSFSGASWVWWDLVFVPASGTILTPGTYDWVTRWPFHSP
                     TTGGLSVVGDGRGCNILVGRFTVLEAEYGEGGEVLRLAVDYEQHCEYDDAALFGSVRY
                     HSAVPLGPRLAVGLASRFEGDEGPSALDFVVSLSGPADRVVSVNYATVAETATDGSDY
                     AGASGTASFPVGETQVIVTVPVLGDVVEEADETLSVALSGDSGPPIAFDRGVGTILND
                     DPYKTYLSFDSEVYDWVGLGQRFTLTPLEGNITLERVGHAVHVEFYGSTVWSLRFAPS
                     AGALLTPGAYEGATFYPFQSPTTPGLNVFGDGRACGMVDGRFTVREAEYGTSGEVLRF
                     AADYEQHCHLSGPALFGSVRFRSSVPREARVSVGSASLYEGDSGSSNMTYVISLSAPV
                     ATAVTVDYTTVDGTARAGVDYSAVSGTGTFPPGETAVPVTVPVLGDVQEEGDEWLALA
                     LSNAAGAPIGFDEGEGTILDDDPYKTLLHFNSEAGDSIGKGRRFTLTPADGGIAAERV
                     DGGVHIAYKSEWNWDLHFVPPAGLPLAPGDYFGAHQWRHGASTMPQMAVFGDGSACSL
                     LDGHFTVLEAEYGPDGEVRRLAVDFEQHCEGRAPALFGWVRYDSSVPVVPRLEVSSFS
                     RYEGDAGTWTMNFDISLPAPAENVVTVDYRTAGVTASAGSDYVAVSGTATFPVGTTKV
                     TVPVMVLADKVMENDETFEFVLSNSVGTPIGFARGVGTILNDDPYKTFLYFDSEPGDF
                     IGDGRRFTLGWEDGVITAESREHGVHVQFRGLSSWDLTFRPRSGERLRPGVYAEAERS
                     STPTAPGLDVNGDGRGCNAVTGRFRIHELEFRTNGEVVRFAADYEQHCEGGVPALFGA
                     VRYKSGVPRVRRAWVGNSTVTESVVTGSPPNRTGTSLEGARPHSLAPSGRRAAAFGKG
                     TE"
     gene            4689..5114
                     /locus_tag="PLP50_15770"
     CDS             4689..5114
                     /locus_tag="PLP50_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53051.1"
                     /translation="MYHPDIFPFRTCNWGVGPMLYADRSLFEPGRPQHLLMLNPRRTR
                     YRTNLVIVNGDEEEASVSQEVLSAAGRSATLTFQVPGKTYHQLNDIYALPELESLELD
                     SQDIRFFGQRAVVICSSHCFAIAYAISNENNTVSIVEPR"
     gene            complement(5231..>6101)
                     /locus_tag="PLP50_15775"
     CDS             complement(5231..>6101)
                     /locus_tag="PLP50_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HPA53052.1"
                     /translation="PEEVNQDKLRFAEKLLEEAEHDLSRLGLILDVLKIQNVADEVGY
                     LNSLGRKQTAALDKSARVAEAKAHAEASVREAENNLSARLALVDAEGAIAQAEADKRI
                     TQCVTGQEAMIAEEVGKVTAAIAKAEAELKVQKARLEQVRLKLEADVVAPARADMEKK
                     QAEARGRAAKIVEDGRATAAVLTQMIETWKAAGPNARDIFLMQKLQSVMSSLVETIGS
                     VKVDRVAYLPASAKVAQGVQLVEQVKAAFGVDLAKVVGRLGAPEEAPVPAKLTAAEAL
                     GATAQTAPARPKT"
BASE COUNT          964 a         2048 c         2095 g          994 t
ORIGIN      
        1 ggagctgggc cggaggctcc tgggagaggg agcctagccg gaaggtcctc aggaaaacgc
       61 atcttgaatt ggaccttggg ccttgtggct gagggtgttc ctgctcttct ctgctccaga
      121 tgtggggatt ggcgtcttcg tattcgacgg cagggcggca gagagcgaga gcgggcacga
      181 acgggctcga acgcccgggg gagcggcgcc gcggcggcgc cgttcctcag ggcgaggtat
      241 cgcggggtgc tggtgcagtc tccgggcgat cctccaggag aatgcggcga gcttctcggg
      301 gtcggtcggt ggtcgaggtg gcctcggcct gcctggcaca tggaggtatc ggccccggcc
      361 gccaaagggg gatcagaggg aatcgagaac cggggcggca gccagaggcg gagcgcgact
      421 tcggacggca cggcgagccg aggcgccagg atggtcgagg aggcggcgga tgacgcgggg
      481 ctcggtgatc gtcgcgaacg aggtgacttc tcggttcggg gacgtgggcg aggagacgga
      541 cgacgaggtc gagggggtcg agcacctgcc ggatccccgt cgagcccggc atcgcgaaag
      601 gcgagcaggc gtccgtccgc ccatggcatc gaccgagcta ctcggcggga gccgattcct
      661 ccagggcgcg ccccgacgcg tcgtaggagt agcgtgtccg agtgccggtc cccttacccg
      721 tctcgtcgat ccggatctcg gtcttcagcc tgatccgacc ctccccgtcg aaacgctgct
      781 cggttctccg ggtgagacgc ccggaggcgg tgtgctcctc ggcgatgacg agagagggcc
      841 gtaaggaatc gaggtcgtac tcgagacggg tgcgcagccg ggccccgccc gcgatcgtcg
      901 tttcgcgcga ggtcaccctc cccttcctgt cctttgtgac gcgttcgtcc ggcctcgact
      961 ccgcgaacgc ggccccggtc agggcgagca ggaataagcc gagcagccag acggcgaccc
     1021 tggacggcgc ttcttcccat actcgcgtca tgagcgtcgg ctcgagcaga acggcggttc
     1081 cgtccccacg cgccgggcac aaccccttgt ggacatcgtg cgggacgccg ctcattcggt
     1141 ccccttcccg aaggcggccg ctcgccttcc cgatggcgcg agcgagtgcg gtcgagctcc
     1201 ttcgagggac gtgccggtcc tgttcggggg agacccggtc acgacgctct ccgttacggt
     1261 ggagttcccg acccacgcac gacggacccg gggcacgccc gacttgtagc gcacggcgcc
     1321 aaaaagcgcc gggacgcctc cttcacagtg ctgctcgtag tccgccgcga atctcacgac
     1381 ctccccgttc gtccggaact ccagctcgtg gatcctgaaa cggccggtca ccgcgttaca
     1441 gccgcgcccg tccccgttta cgtcgagccc gggcgccgtc ggcgtgctgg aacgctccgc
     1501 ctccgcgtag acaccaggcc gcaggcgctc tccggagcga ggcctgaagg tgaggtccca
     1561 cgacgataga ccgcggaact gaacgtggac tccgtgctcg cgggactccg cggtgatgac
     1621 cccgtcctcc cagccgagcg tgaaccgcct gccgtcgccg atgaagtcgc cgggctcgct
     1681 gtcgaagtaa aggaacgtct tgtagggatc gtcgttcagg atcgtgccga caccgcgcgc
     1741 gaagccgatc ggcgttccca cgctgttgct cagcacgaac tcgaacgtct cgtcgttctc
     1801 catgaccttg tcggcgagga ccatcacggg cacggtcact ttcgtcgtgc cgacggggaa
     1861 cgtggccgtt cccgagaccg caacgtagtc gctgccggcc gaggccgtga cgcctgcggt
     1921 ccgatagtct acggttacga cgttctcggc gggagccggc aacgagatgt cgaagttcat
     1981 cgtccacgtg cctgcgtccc cctcgtagcg cgagaaggag ctgacctcca ggcgcgggac
     2041 gacgggaacg ctggagtcgt agcgaaccca gccgaacagc gcgggggcac gtccctcgca
     2101 gtgctgctcg aagtcgaccg cgagcctccg gacctccccg tcagggccgt attcggcttc
     2161 gaggaccgtg aagtggccgt ccagcaggct gcaggcggag ccatcgccga agacggccat
     2221 ctggggcatc gtcgaagcac cgtggcgcca ttggtgcgcc ccgaagtagt cgcccggcgc
     2281 cagcggaaga ccggccggtg ggacgaagtg gaggtcccag ttccattcgc tcttgtaggc
     2341 gatgtggaca cctccgtcga cgcgttccgc ggcgatgccc ccgtccgccg gcgtgagagt
     2401 gaaccgacgt cccttgccga tcgaatcgcc ggcctcgctg ttgaaatgaa ggagagtctt
     2461 gtaggggtcg tcgtcgagaa tcgtcccctc gccctcgtcg aagccgatgg gcgccccggc
     2521 ggcattgctc agcgcgaggg cgagccattc gtcgccttcc tcctgcacgt cgccgagcac
     2581 cggtaccgtc accggcaccg ccgtttcacc gggagggaag gtgcccgtgc ctgagacggc
     2641 agagtagtcg acgcctgctc tggccgtccc gtcaacggtc gtgtagtcca ccgtaacggc
     2701 ggtcgctaca ggagccgaca gcgagatcac gtacgtcatg ttcgaggagc cgctgtcccc
     2761 ctcgtagagt gaggccgatc cgaccgagac gcgggcctcg cgcggcacgc tcgagcgaaa
     2821 gcgcaccgac ccgaacaggg ccggtcccga aaggtggcag tgctgctcgt agtccgccgc
     2881 gaaccggagg acctccccgc tcgttccgta ctcggcctcg cgaacggtga agcgtccatc
     2941 gaccatgccg caggctcgcc catccccgaa gacgttgaga ccgggggtcg tcggagactg
     3001 gaacgggtag aacgtcgccc cttcgtaggc tccgggcgtc agcagcgctc cggccgacgg
     3061 ggcgaatcgg agactccaga cggtcgagcc gtagaactcg acatgaacag cgtgtccgac
     3121 tctctcgagc gtaatgttcc cttccagggg tgtcagcgtg aaccgctggc cgagaccaac
     3181 ccagtcgtaa acctcgctat cgaaggagag gtacgttttg taggggtcgt cattgaggat
     3241 cgtcccgact ccccgatcga aggcgatcgg cggtcccgag tcgccgctca gcgcgacaga
     3301 gagggtctcg tcggcttctt caacgacgtc gcccagcacc ggcaccgtca cgatcacctg
     3361 ggtttcgccc acgggaaagc tcgccgtgcc ggaggccccg gcgtagtcac tgccgtccgt
     3421 ggcagtttcg gccaccgtcg cgtagttgac ggagacgacc ctgtcggccg ggccggagag
     3481 ggagacgacg aaatccagcg ccgacgggcc ctcgtcgccc tcgaaccgac tggccaggcc
     3541 gacggccagg cgcgggccca ggggcacggc ggagtggtag cgcactgatc cgaacagcgc
     3601 cgcgtcgtcg tactcgcaat gctgctcgta gtcgaccgcg agtctcagga cctctccacc
     3661 ctctccgtat tcggcttcga gaaccgtgaa gcggccgacc aggatgttgc agcctctccc
     3721 gtccccgacg acgctgaggc cgcccgtcgt cggggagtgg aacggccagc gcgtcaccca
     3781 atcgtatgtg cccggcgtca ggatcgtccc cgaagccggg acgaagacga ggtcccacca
     3841 gacccacgaa gcgccgctga acgagacgac gacaccgccg tcgacccggc tggccacgat
     3901 ctgcccgtcc gacggagtca gcgtgaactg accgccctgg ccgatccagt cgtctggttc
     3961 gctattgaac tggagcagcg tggtctcctg cgccgatgcg gctccgcagg cggccagcaa
     4021 gagcccgagg aaagcgcaat gcgctgccag cttcacttgg gacaggatag cgcaagacag
     4081 tacgtcgact gcgattcacg gcccacgcga acgcgtccga gtccggaagt cgcgccgcag
     4141 ttttcgaggt tgatgcggcg gccgctccgc aaccagtgcg cgcggccgtc cgcggcgtca
     4201 gccggcttcg gcccggtccg tggaggggtc gctcggcgag agcgccgtcg tcgaggcaga
     4261 tccacgactg ttggctcgca cgccatctcg tgaccgttcc cgggcaattc cgccgacgga
     4321 gaaatgcggg atggcggcat gccatgccaa cgtccccgct gtagaagccg tggagatcgc
     4381 atggaaggcc cacgtgctca gcagcatgag cggcgattcc caccgctccc aaagcgctgc
     4441 cactaccgac tcttactccc gggtctgtga tcaacccgtg accgcggcgg tcacgagata
     4501 agtcgagagc caacacttgg atttggaccg aaggcggttt ctaccccaca ggccaggccg
     4561 agtacggtgc tgcggtctgt gaggccgaag atgggctgat cgtcgtatcg aggaccctga
     4621 cgagcacggt gcgcgaaccc ggcggccccg atagcccctg gacctgctag ggtaactctg
     4681 gcgggttcgt gtaccacccg gacatcttcc cgttccggac ctgcaactgg ggagtcggtc
     4741 cgatgctgta cgcggaccgg agtctcttcg agccgggccg tcctcagcac ctcctgatgc
     4801 tgaacccgcg ccgaacccgc taccggacga acctcgtcat cgtgaacggc gacgaggagg
     4861 aggcctcggt ctcccaggag gtcctgtctg cggcgggacg gagcgcgacg ctaacgtttc
     4921 aggtgccggg caagacgtat caccagctga acgacatcta cgcgctcccg gagctcgagt
     4981 cgttggagct ggactcccag gacatccgct tcttcggcca gcgggccgtc gtcatctgct
     5041 cgagtcactg cttcgcgatc gcttacgcaa tctcgaacga gaacaacacg gtcagcatcg
     5101 tcgagccgag gtagggtgtt ggcccgacgg gatactccag cgtagcgcca tacggctccg
     5161 tcggagggcg ccgggcgggg gtcgattccc ccgccgcggc gcctccgcca gtctcggccg
     5221 gatcgggccg tcaggtcttc ggtctcgccg gagctgtctg cgccgtcgcc ccgagggcct
     5281 ccgccgccgt gagcttcgcc gggacgggcg cctcctcggg cgccccgagc ctcccgacga
     5341 ccttcgcgag gtcgacgccg aaggcggcct tgacctgctc gacgagctgg acgccctgcg
     5401 cgaccttcgc ggaggcgggg aggtaggcga cgcggtcgac cttgacgctc ccgatcgtct
     5461 cgacgagaga cgacatgacg ctctggagct tctgcatgag gaagatgtcg cgggcgttcg
     5521 ggccggcggc cttccaggtc tcgatcatct gggtgaggac ggcggcggtc gcccggccgt
     5581 cctcgacgat cttcgcggcc cggccgcgcg cctcggcctg cttcttctcc atgtcggcgc
     5641 gggcgggggc gacgacgtcg gcctcgagct tgaggcggac ctgctcgagg cgcgccttct
     5701 gcaccttcag ctcggcctcg gccttcgcga tcgcggcggt caccttgccg acctcctcgg
     5761 cgatcatggc ctcctgtccc gtcacgcact gggtgatccg cttgtcggcc tcggcctggg
     5821 cgatcgctcc ctcggcgtcg acgagagcga ggcgggcgga gaggttgttc tcggcctcgc
     5881 ggacggaggc ctcggcgtgg gccttggcct cggcgacgcg ggcgctcttg tcgagcgcgg
     5941 cggtctgctt gcggccgagg gagttgaggt agccgacctc gtcggcgacg ttctggatct
     6001 tcaggacgtc gaggatcagg ccgaggcggg agaggtcgtg ctcggcctcc tcgaggagct
     6061 tctcggcgaa gcggagcttg tcctggttga cctcctcggg g
//
LOCUS       DAOONL010000171         6075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15484_length_6075_cov_19.161462, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000171 DAOONL010000000
VERSION     DAOONL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15484_length_6075_cov_19.161462"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLP50_15780"
     CDS             <1..899
                     /locus_tag="PLP50_15780"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPA53053.1"
                     /translation="YRGYDVWELPPNTQGLAALQMLNLLEGFDLAKMGRDSADFWHVM
                     VEAKKVAYADRARYYADPAFAKLPLERLLSKEYAKERAKKIDMRRASREDPPDEELVL
                     NRKETTYLCAADASGLMVSLIQSNYTGFGSGYAVPALGIGIHNRGGLFSLRKGHPNYL
                     EPGKRPFQTIIPAFLTKDGQPLMAFGLMGGDMQPQGHVQVVVNLVDFDMNLQEAGDAI
                     RFHHTGSSEPTGTIMKDGGTLYLEEGLPEPVLEELMRRGHRIGREPVGAYGGYQAVAR
                     DPATGFLLGATEKRKDGAAAGF"
     gene            964..2190
                     /locus_tag="PLP50_15785"
     CDS             964..2190
                     /locus_tag="PLP50_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPA53054.1"
                     /translation="MSVSAEKAVPRVSERGRRVPASPIRKLASYADAAKKRGVKVYHL
                     NIGQPDLETPAPMRDRLAEIKDKVYAYTPSNGTAECLDFLVGYYRGRGVELTKSQVIT
                     TTGGSEAIQFAFMACCEVGDEALLVEPFYTNYRAFAAMGGVELKPIVTRAEDGFHLPS
                     REEWEKALTPKTKAVLLCNPNNPTGTVYSKEELVLVAEFCRDHGLFLIADEVYREFVY
                     DGRKVESALSLPGFEDVVVVVDSLSKRYSACGIRLGCLVTRNKDVYQACLHMAQGRLS
                     PPGLAQAIAPGAALLGPDYVEGVVKEYGHRRDILYEELTKIPGIFFRKPEGAFYFVAR
                     IPVKDSEDFAQWLLTDFQLDGATVMVAPADGFYATPGLGKDEVRIAYVLKAEDLKAAC
                     RVLEAAIPAYRAARGL"
     gene            2474..3067
                     /locus_tag="PLP50_15790"
     CDS             2474..3067
                     /locus_tag="PLP50_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53055.1"
                     /translation="MSYPAALIVVLGLLSAACGPARVPSAGPQSSHPGSESIQPIPPP
                     RLAAPTALAGSPAARPLEALDAAGLAPVELTAVAVEGFPPLPSYRAKVRNLSDRPVRR
                     VLATVVYLDAAGRPMPGEEHDVSFGSPLKAIDPGVTLEASFLSRVERAPGVRLVVRSI
                     TFLEKGSGAEPGTREWANPRYDADLAALRGASPGEPR"
     gene            complement(3214..>4594)
                     /locus_tag="PLP50_15795"
     CDS             complement(3214..>4594)
                     /locus_tag="PLP50_15795"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA53056.1"
                     /translation="RPASTSPWRIGLEGAAVSVPLFVTLLHLALFAFDRRARENLYYA
                     GQMAISAVLILREFRASLVPGLLERELLDRWGSGLPALASLFGLLTYYTVRTRPFPRS
                     ARPLLAAGVFLFGAAYVWDPAAEHGWIVYFAVVVLEVVRLERRGRVVPREGMGFSLAG
                     MAVFGVAIALQVMVIWGWVESVAGLRTVFALGALGSAIGMSLFLARTLGQTRLLEAEN
                     ERKGRELERARELQLSMLPTELPSATGLDVAAVTAPAAEVGGDYYDFRRDGEGLLVAF
                     GDATGHGLASGLVVTAVKALFVSVPVDGPLPRLLTACDRALGAMRLRNVHMCLALARI
                     TRDEAVLVSAAMPPTLVHRAASDVVEELGAGGLPLGSRLRPSWEERRTRLGPGDTLLL
                     ASDGFPELVGEDERVLGFLGAAEAFRLAARAADARGVVAGLQSSAAAWRGARAPDDDM
                     TFVVVRVRR"
     assembly_gap    4595..4694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4700..5113)
                     /locus_tag="PLP50_15800"
     CDS             complement(4700..5113)
                     /locus_tag="PLP50_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane spanning transport protein"
                     /protein_id="HPA53057.1"
                     /translation="MSGGRGGKDSTAEARAITVARLVLGTLFASTGLCGLLGLLPLAP
                     PHAFQQILLDSGWMVFVKLVELAAGLLLLSNRFVPLALALLAPVVGSIALYHLLLDPA
                     FLWLVPLVVGLEGFLLWAYRGSFRGVLARDARPGG"
     gene            complement(5110..5595)
                     /locus_tag="PLP50_15805"
     CDS             complement(5110..5595)
                     /locus_tag="PLP50_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPA53058.1"
                     /translation="MTSIADRLATGLGLEPHPEGGRYRQLHRSGSVVHVPSRGVERAA
                     LTSILFLLSEGEVSRWHRVLSDEVWHFHEGDALELLVVDAALGEVRRHVLGRASDGNE
                     PLAVVPTGAWQAARPLGDYALVACDVGPGFDFADFALLAELPDEAARFRERFPVLSGL
                     V"
     gene            complement(5597..>6075)
                     /locus_tag="PLP50_15810"
     CDS             complement(5597..>6075)
                     /locus_tag="PLP50_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53059.1"
                     /translation="REGYEVLALDPDPAAVAALRELARRLAPSLPEASVRCERVDAST
                     FPDACADVVISNAVLHFAPDEETFLAMLRGTWRLLAPGGLLFCRLASTIGIERLVRPL
                     GGRRFALPDGTDRFLVDEAYLLERTTELGGRLLDPIKTTVVQGQRAMTTWVVGKAG"
BASE COUNT          954 a         2195 c         2069 g          757 t
ORIGIN      
        1 gctaccgcgg ctacgacgtc tgggagctcc cgccgaacac gcaggggctc gcggcgctcc
       61 agatgctgaa cctcctcgag ggtttcgacc tcgcgaagat ggggagggac agcgccgact
      121 tctggcacgt gatggtcgag gcgaagaagg tggcgtacgc ggaccgggcc cgctactacg
      181 ccgaccccgc cttcgcgaag cttcctctcg agcgcctcct ctcgaaggag tacgcgaagg
      241 agcgggcgaa gaagatcgat atgaggcgcg cctcgcgcga agaccctccc gacgaggagc
      301 tcgtcctcaa ccggaaggag acgacctacc tctgcgcggc cgacgcctcg ggcctcatgg
      361 tctccctcat ccagagcaac tacaccggct tcggctcggg ctacgcagtc ccggcgctcg
      421 gcatcggcat ccacaaccgc ggcggcctct tctcgctgag gaaggggcac ccgaactacc
      481 tggagcccgg aaagcgcccc ttccagacga tcatcccggc cttcctgacg aaggacgggc
      541 agcccctgat ggccttcggc ctgatggggg gcgacatgca gccgcagggg cacgtgcagg
      601 tcgtcgtgaa cctcgtcgac ttcgacatga acctccagga ggcgggcgac gcgatccgct
      661 tccaccacac cggctcgagc gagccgacgg ggacgatcat gaaggacggc gggacactct
      721 atctcgagga ggggctcccc gagccggtcc tcgaggagct gatgagacgc ggtcaccgga
      781 tcgggcggga gcccgtcggc gcctacggcg gttaccaggc cgtcgcgagg gacccggcga
      841 cgggcttcct cctcggggcg accgagaagc ggaaggacgg ggccgccgcc gggttctgat
      901 tggtcgggcg agccggtccc gcccggcagc ggaggcgcgg atgcggggtg taaccttctc
      961 cccatgagcg tctccgccga gaaggccgtt ccccgcgtct ccgagcgggg ccggcgtgtt
     1021 cccgcctccc cgatccgcaa gctcgcctcc tacgccgacg cggccaagaa gcgcggcgtg
     1081 aaggtctacc acctgaacat cggtcagccc gacctcgaga cccccgcccc gatgcgggac
     1141 cggctcgccg agatcaagga caaggtctac gcctacaccc cgtcgaacgg caccgccgag
     1201 tgcctcgact tcctcgtcgg ctactaccgc ggacgcggcg tcgagctgac gaagagccag
     1261 gtcatcacga cgaccggcgg gagcgaggcg atccagttcg cgttcatggc ctgctgcgag
     1321 gtgggcgacg aggcccttct cgtcgagccg ttctacacga actaccgcgc cttcgcggcg
     1381 atgggcggcg tcgagctgaa gccgatcgtc acccgcgccg aggacggctt ccacctcccc
     1441 tcgcgcgagg agtgggagaa ggccctcacg ccgaagacga aggccgtcct cctctgcaac
     1501 ccgaacaacc cgaccgggac ggtctactcg aaggaggagc tcgtcctcgt cgccgagttc
     1561 tgccgcgacc acggcctctt cctgatcgcc gacgaggtct accgcgagtt cgtctacgac
     1621 ggacggaagg tcgagagcgc cctgtcgctt ccggggttcg aagacgtcgt cgtcgtcgtc
     1681 gactccctct cgaagcgcta cagcgcctgc ggtatccgcc tcggatgcct cgtgacgcgg
     1741 aacaaggacg tctaccaggc gtgcctccac atggcgcagg ggcggctctc ccctcccggc
     1801 ctcgcgcagg cgatcgcgcc cggcgcggcc ctcctcggcc ccgactacgt cgaaggggtc
     1861 gtgaaggagt acgggcaccg gcgcgacatc ctctacgagg agctgacgaa gatccccggc
     1921 atcttcttcc gcaagccgga aggtgccttc tacttcgtcg cgcggatccc ggtgaaggac
     1981 agcgaggact tcgcccagtg gctcctcacc gacttccagc tcgacggcgc caccgtcatg
     2041 gtcgctcccg ccgacggttt ctacgcgacg cccgggcttg gcaaggacga ggtccggatc
     2101 gcctacgtcc tgaaggccga ggacctgaag gccgcctgcc gcgtcctgga ggcggcgatc
     2161 cccgcctacc gcgccgctcg ggggctctga cccgagacct cgcggagcac atcgttcggg
     2221 cgtgtgccgg ctcattcgag ccggcacttg cttttctgcc gtctgggtcc caccttctcg
     2281 tcaagggaca gccaactcca gagagttcgt ctcatcgaca gacctgatct cccctggccg
     2341 gcgaggaacc actctccgcc gcccccctcc ccgccaggcc agggacgccg ggagcgtcgc
     2401 cccattggca cgctcccggc agctttcttc gagcgagcga cggacggaca gtctccgatg
     2461 taaggtctgg cgcgtgtcgt accccgctgc gctcatcgtc gtgctcgggc tcctgagcgc
     2521 cgcttgcggc cccgcccggg ttccgtccgc tgggccacaa tcgtcgcacc ccggctcaga
     2581 gagcattcag cccataccgc cccccaggct cgcggcaccg acggcactcg ccgggagtcc
     2641 ggccgcccgg cccctggagg cgctcgacgc cgccggcctt gcgccggtcg agctgaccgc
     2701 cgtcgccgtc gagggcttcc caccgctccc gtcatacagg gcgaaggtcc ggaacctctc
     2761 ggaccgcccc gtccgtcgcg tcctcgccac ggtcgtctac ctcgacgccg ccggccgccc
     2821 gatgcccggc gaggagcatg acgtctcctt cgggagcccg ctgaaagcga tcgaccccgg
     2881 cgtcacgctc gaggccagct ttctctctcg cgtcgaacgg gcgccgggcg tgcgcctcgt
     2941 cgtccgttcg atcacgttcc tcgagaaggg ctcgggggcc gagccgggga cgcgcgaatg
     3001 ggcgaacccc cgctacgacg ccgacctcgc ggcgctgcgc ggagcgtccc cgggcgagcc
     3061 gcgctgatct cgggcgaccg gacgaggact cgacgcggga cgtcctctcg atctcgacgt
     3121 cggtcccccg gcgcgacgcg cgggcgggcc tcgacctcgg cgcggccggc cgcggggtgg
     3181 atgaacgccc gcttcgggtc cgcggcccct cggtcagcgc cgaacgcgga cgacgacgaa
     3241 cgtcatgtcg tcgtccgggg cacgggctcc tcgccaggcg gcggcgctcg actgcagccc
     3301 ggccacgacc ccgcgcgcgt ccgcagcccg ggcggcgagg cggaacgcct ccgccgcccc
     3361 gaggaatccg aggaccctct cgtcctcgcc gacgagctcc gggaacccgt cactcgcgag
     3421 aagcagggtg tctcccggac cgagccgcgt ccgccgctcc tcccacgaag gtctcaggcg
     3481 gctgcccagg ggaagccccc cggccccgag ctcctcgacg acgtcgctcg ccgcgcggtg
     3541 gacgagcgtc ggaggcatcg cggccgagac gaggacggct tcgtcccgcg tgatccgggc
     3601 gagcgccagg cacatgtgga cgttccggag gcgcatcgcg cccagcgccc ggtcgcacgc
     3661 ggtcagcaga cgcgggagcg gcccgtccac ggggaccgag acgaagaggg ccttcaccgc
     3721 cgtcacgacg aggcccgagg cgaggccgtg cccggtcgcg tcgccgaacg ccacgaggag
     3781 gccctccccg tcgcggcgaa agtcgtagta gtcgcctccc acctccgcgg ccggagccgt
     3841 cacggcggcg acgtccagcc ccgttgccga gggaagctcc gtcgggagca tcgagagctg
     3901 cagttcgcgg gcccgctcga gctccctccc cttgcgctcg ttctccgcct cgaggagacg
     3961 ggtctgtccc agcgtccgcg ccaggaagag cgacatgccg atcgcggagc cgagggctcc
     4021 gagggcgaac acggtgcgca accccgcgac ggactcgacc cagccccaga tcaccatcac
     4081 ctggagggcg atcgcgacgc cgaagacggc catccccgcg agggagaacc ccatcccctc
     4141 gcgcgggacg acgcggcctc gccgctcgag cctcacgacc tcgaggacga cgacggcgaa
     4201 gtagacgatc cagccgtgct cggccgcggg atcccagacg taggccgcac cgaaaaggaa
     4261 gacgccggcc gcgaggagcg gtcgagccga gcgggggaac ggtctcgtcc ggaccgtgta
     4321 gtaggtgagg agaccgaaga ggctcgcgag cgccggcagg ccgctccccc accggtcgag
     4381 cagctccctc tccagcaggc ccgggacgag gctcgcgcgg aactccctca ggatgaggac
     4441 ggcggagatc gccatctggc cggcgtagta gaggttctcc cgcgcgcgcc ggtcgaacgc
     4501 gaagagggcc aggtggagga gcgtgacgaa gagcgggacc gagacggccg cgccttcgag
     4561 gccgatcctc cagggcgagg tcgaggccgg ccgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnngatcct cacccgccgg ggcgggcgtc gcgcgcgagg acgccgcgga
     4741 agctccctcg atacgcccac agcaaaaacc cctccagccc cacgacgagc ggcacgagcc
     4801 agaggaacgc cgggtcgagc aggaggtggt agagggcgat cgagccgacg accggcgcga
     4861 ggagggcgag cgcgagcggg acgaagcggt tcgagaggag gaggaggccg gcggcgagct
     4921 cgacgagctt cacgaacacc atccagccgc tgtcgaggag gatctgctga aacgcgtgcg
     4981 gtggcgcgag cggcaggagg ccgaggagcc cgcagaggcc cgtcgacgcg aagagggtgc
     5041 cgaggacgag gcgggcgacc gtgatcgccc gagcttccgc ggtagagtcc ttcccgcccc
     5101 tcccgccgct cacacgagcc ccgacaggac ggggaaccgc tcccgaaaac gcgccgcctc
     5161 gtccggaagc tccgcgagga gcgcgaagtc ggcgaagtcg aagccgggcc ccacgtcgca
     5221 ggccacgagg gcgtagtcac ccagcggccg cgccgcctgc caggcgccgg tcggaacgac
     5281 ggcgaggggc tcgttcccgt cgctcgctcg cccgaggacg tggcgcctca cctcccccaa
     5341 ggccgcgtcg acgacgagaa gctcgagagc gtctccctcg tgaaagtgcc agacctcgtc
     5401 ggagaggacc cggtgccagc ggctcacctc gccctccgaa aggagaaaga ggatcgacgt
     5461 gagcgcggcg cgctcgacgc cgcgcgacgg cacgtggacg accgacccgg acctgtggag
     5521 ctggcgatag cgccccccct cggggtgcgg ctcgagaccg aggcccgtgg cgagacggtc
     5581 cgcgatcgac gtcacgtcag cccgccttcc cgacgaccca cgtcgtcatc gcacgctgcc
     5641 cctgcaccac cgtcgtcttg atcgggtcga gaagccgccc tccgagctcg gtcgtccgct
     5701 cgagcaggta cgcctcgtcg acgaggaatc ggtccgtccc gtcggggagc gcgaagcgcc
     5761 gcccgccgag cggtcggacg agccgttcga tcccgatcgt ggaggcgagg cgacagaaga
     5821 gaagccctcc cggcgcgagg agccgccagg tgccgcggag catcgcgagg aacgtctcct
     5881 cgtcgggcgc gaaatggagg acggcgttcg agatcacgac gtcggcgcag gcgtcgggga
     5941 acgtcgacgc gtcgacccgc tcgcagcgga cactcgcctc cgggagcgac ggcgcgagcc
     6001 gtcgcgcgag ctcgcgcagc gccgcgacgg ccgccgggtc cgggtcgagc gcgaggacct
     6061 cgtacccctc gcgga
//
LOCUS       DAOONL010000172         6044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15599_length_6044_cov_11.185841, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000172 DAOONL010000000
VERSION     DAOONL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15599_length_6044_cov_11.185841"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLP50_15815"
     CDS             <1..1113
                     /locus_tag="PLP50_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53060.1"
                     /translation="HAKVIRFFSLKPKREVWFVGSANLTRAAFSGTANRETGFLVELD
                     DIPPGPLAFWLDIDEKKPKEFEIGTDPDEPAASGGTRLQVQHDWSSGETKAFWDGPKA
                     SPALVVSANGVEVFKVEGLPSRAWTALPEEDSKKLAERLKSTSIFEVAGEDPEGKPGL
                     LLVQEIGMFRKPSLAPERTAADILKDWAAPSDDRRVALYEEGLLGVPEPGELEPRPSD
                     DGLVPVESLFDRFAGIFHGFSSLEKRVVEQLNGKNDDGAAYLVFGETFNSLATFLRKE
                     KEEEIERDPVNVYVAYLCAKQLVATVRKEAGALLGSTFLDRHGSALKALEAQVEEPLG
                     GLRQLISDEIGADGAKFLEWFDRWFLKRATPVGEGA"
     gene            1110..>4148
                     /locus_tag="PLP50_15820"
     CDS             1110..>4148
                     /locus_tag="PLP50_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA53061.1"
                     /translation="MIDVETARQLLDFAARLPNDKGGRAEQQLEGAVALHNILKTHGV
                     AYLADEVGLGKTYVALGVVALLRHFDPALRVLVIAPRENIQRKWMKETRNFIRHNVRH
                     ADLRVRELDGNPSRPLVMCGNLAELVREASLDPRRDFFARMTSFSIGLTGDAREASRE
                     AKEKLRDQVREAIPWVQDDALSLKLGKAGFKDAVAQATCCALPEFDLVIVDEGHNLKH
                     GFTEKGAARNRVMGLVFGHPSARPDPGLFPKYGPRAKHVLFLSATPVEESYRHLWNQL
                     DVFGKGDRFPLLNDPEAGEGKKREEARQFLIRRVTEINVGGERHTKNLYRREWRFGGV
                     DRHDNPIEVSDLRGRLVVALVQKKVMELLGSEKFNSSFQIGMLASFESFLETSRLKEK
                     TAAEGEEEETKVVVFDDADQTEDPLEREGLDVHALNRLAKDYRKQFDAELPHPKMDAL
                     VRTLADRWTSGRKALVFVRRIGSVKELKAKLDAEYDTWLKDRLLRRLSGEARSTLEQW
                     WERYREERSRRRASDAADDSAPAPARPDREKPDRGGIDTFFAWFFRGEPKPGVFSGAL
                     LKESFTRPGSVNSTFFEVNDVGRMLGAPSGKVLERLAEQVGLPVEEARARVNELGRNW
                     LGAKALRLHQFEAAQVGALRLLAERAAKPLADEAEILRAEKFPAPRSESRAREGADFA
                     ARLEERTFFSELIGNETLRADLWPEPAETLALRDRVREREQRAILLATAARLGHSLID
                     LYILAVKRRGNLNPGRAEEDAEEREDAAVAEAGDDSLIDDFLRLLEGQRTLPPGFGWF
                     GAYDELSAIGRNFPLIVDVNIPNIAEMPLAATAARFGRLLGEQQPVAGMSGQVNMTLV
                     QQFRMPGYPFVLVSTDLLQEGEDLHTFCSDVFHYGISWTPSSMEQRIGRVDRVRSQTE
                     RRLREADRKPEGNDKLQVYYPYLSDTVEVLQVVRVLKRMDTFLRLMHEDLALPEFDQK
                     RLSISHEIMGLPRPPEPIPTPLESAFPVRKDVHLNGKGKKL"
     assembly_gap    4149..4248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4249..4819
                     /locus_tag="PLP50_15825"
     CDS             <4249..4819
                     /locus_tag="PLP50_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53062.1"
                     /translation="SAFPVRKDVHLNGKGKKLVVDAETEKAVRERLERMCTPRVGKLA
                     VEWQVNSPPGQKLGIARLGTRQQPFVLEVQGRDQGLLLRCSSHVGQIGLKAQQQKLLD
                     AVATKGGRIQVQKVGKKGEWLVSLAEDVLLAGEVHDAARAAWLVERVVCGADDLEIEL
                     LGDKDASLEDMVEARKHHGRARHEGDANA"
     gene            4812..5207
                     /locus_tag="PLP50_15830"
     CDS             4812..5207
                     /locus_tag="PLP50_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53063.1"
                     /translation="MPDLSDLLAATDMKVTGRKVKVSFPNGRSHRVSVSEKPDAWELR
                     GVVLSADTVSEQYSDDDSDRLGLALRAWQRNGTARLCGFRLDSDGALVGEATVPKIGL
                     TAEELTEMARAVARACDLLEAHLTGEDEE"
     gene            5287..5514
                     /locus_tag="PLP50_15835"
     CDS             5287..5514
                     /locus_tag="PLP50_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53064.1"
                     /translation="MSHRLQILVPKPLDARIVKAASRRRMSKGAWVRKVLETALDQET
                     SGEDPLARLSALGAPTADIHQMLSEIEAGRK"
     gene            5511..5915
                     /locus_tag="PLP50_15840"
     CDS             5511..5915
                     /locus_tag="PLP50_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA53065.1"
                     /translation="MTVFIDSNIPMYVAGRPHPNREPSRRFLERIRAGEVDGVTSTEV
                     LQEILFRYAGLGQLELGLQVYDLFVQICARVLPVTLADTDRARALLTSLTGVSVRDTI
                     HASVMLTNGISRIATFDEGFDRVPGVRRAKLE"
BASE COUNT         1006 a         1974 c         2114 g          850 t
ORIGIN      
        1 cacgcgaagg tgatccgctt cttctccctg aagccgaaac gggaggtctg gttcgtcggc
       61 tccgcgaacc tcacgcgggc ggccttcagc ggcacggcga accgcgagac gggcttcctc
      121 gtggagctgg acgacatccc gcccggcccg ctcgccttct ggctcgacat cgacgagaag
      181 aagccgaagg agttcgagat cgggacggac cccgacgagc cggcggcctc gggcgggacg
      241 cgcctgcagg tgcagcacga ctggtcgagc ggcgagacga aggccttctg ggacggcccg
      301 aaggcctcgc ccgcgctcgt cgtctcggcg aacggggtgg aggtcttcaa ggtggagggg
      361 ctcccgagcc gcgcctggac ggcgctgccc gaagaggact cgaagaagct cgccgaaagg
      421 ctgaagtcga cgtcgatctt cgaggtagcg ggggaggacc cggaggggaa gcccggcctc
      481 ctgctggtac aggagatcgg gatgttccga aagccgtcgc tcgcccccga gcggacggcg
      541 gcagacatcc tgaaggactg ggcggccccg tcggacgacc gccgggtggc gctctacgag
      601 gaggggctcc tcggcgtgcc ggagcccggg gagttggaac cccggccctc ggacgacggg
      661 ctcgtgcccg tggagagcct cttcgaccgc ttcgccggga tctttcacgg cttctcctcg
      721 ctcgagaagc gggtcgtcga gcagctgaac gggaagaacg acgacggggc ggcctacctc
      781 gtcttcggcg agacgttcaa ctcgctcgcg acgttcctgc ggaaggagaa ggaggaggag
      841 atcgagcgcg accccgtgaa cgtctacgtc gcctacctct gcgcgaagca gctcgtcgcg
      901 acggtgcgga aggaggccgg ggcactcctc ggcagcacct tcctcgaccg ccatgggtcc
      961 gccctgaagg ctctggaggc ccaggtggag gagcctctcg ggggcctccg gcagctcatc
     1021 agcgacgaga tcggggccga cggggcgaag ttcctcgagt ggttcgacag gtggttcctg
     1081 aagcgggcga cgcccgtcgg ggagggggca tgatcgacgt cgaaacggcc cgccagctcc
     1141 tcgacttcgc ggcgcgcctc ccgaacgaca agggggggcg cgctgagcag cagctcgagg
     1201 gggcggtggc gctccacaac atcctgaaga cgcacggggt ggcctacctc gctgacgagg
     1261 tggggctcgg gaagacctac gtcgccctcg gcgtcgtcgc cctcctccgg cacttcgacc
     1321 cggcgctgcg cgtcctcgtc atcgccccgc gggagaacat ccagcgaaag tggatgaagg
     1381 agacgcggaa cttcatccgc cacaacgtgc gccacgccga ccttcgcgta cgcgagctgg
     1441 acggcaaccc ctcgcgcccc ctcgtgatgt gcgggaacct cgcggagctg gtgcgcgagg
     1501 cgagcctcga cccgcggcgc gacttcttcg cccggatgac gagcttctcg atcggcctga
     1561 cgggagatgc ccgcgaggcc tcgcgcgagg cgaaggagaa gctgcgggac caggtgcggg
     1621 aggcgatccc gtgggtgcag gacgacgcac tcagcctgaa gctcggcaag gcgggcttca
     1681 aggacgccgt ggcccaggcc acctgctgcg cgctgcccga gttcgacctc gtcatcgtgg
     1741 acgaggggca caatctcaag cacggcttca cggagaaggg ggccgcccgg aaccgcgtga
     1801 tggggctcgt cttcgggcac ccgtccgctc ggcccgaccc gggcctcttc ccgaagtacg
     1861 gcccccgggc gaagcacgtc ctcttcctct cggcgacgcc cgtcgaggag agctaccggc
     1921 acctctggaa ccagctcgac gtcttcggca agggcgaccg cttcccgctc ctgaacgacc
     1981 cggaggccgg ggagggtaag aagcgagagg aggcccgaca gttcctgata cggcgcgtca
     2041 cggagatcaa cgtcggcggg gagcggcaca cgaagaacct ctaccgccgc gagtggcgct
     2101 tcggcggcgt ggaccgccac gacaacccga tcgaggtgag cgacctgagg ggccggctcg
     2161 tcgtggccct cgtccagaag aaggtgatgg agctcctcgg gagcgagaag ttcaattcga
     2221 gcttccagat cgggatgctc gcctcgttcg agagcttcct cgagacctcg cgcctcaagg
     2281 agaagacggc cgcggagggc gaagaggaag agacgaaggt cgtcgttttc gacgacgccg
     2341 accagaccga ggacccgctc gagcgggagg ggctcgacgt ccacgccctg aaccgcctcg
     2401 cgaaggacta ccggaagcag ttcgacgccg agctgccgca tccgaagatg gacgccctcg
     2461 tccggacgct ggccgaccgc tggacctcgg gccgaaaggc gctcgtcttc gtgaggcgca
     2521 tcggctcggt gaaggagctg aaggcgaagc tggacgccga gtacgacacg tggctgaagg
     2581 accggctcct gcgccgactg tcgggcgagg cccgctcgac cctggagcaa tggtgggagc
     2641 gataccgcga ggagcggtcg cggcggcgcg cgtcggatgc ggcggacgat tccgcccccg
     2701 ctcccgcgcg cccggatcgg gagaagccgg accgcggagg catcgacacc ttcttcgcct
     2761 ggttcttccg gggtgagccg aagccgggcg ttttcagcgg ggcgctgctg aaggagagct
     2821 tcacgcgtcc gggaagcgtc aactcgacgt tcttcgaggt gaacgacgtc ggccgaatgc
     2881 tcggagcgcc ttcggggaag gtgctcgagc gcctggccga gcaagtcggc ctcccggtcg
     2941 aggaggcgcg ggcaagggtg aacgagctcg gcaggaactg gctgggcgcc aaggcgctgc
     3001 ggctgcacca gttcgaggcg gcccaggtcg gggccttgag gctcctcgcc gagcgggccg
     3061 cgaagcccct ggccgacgag gcggagatcc tccgcgccga gaagttcccc gcgccccgct
     3121 cggagagccg tgcaagggaa ggcgccgact tcgcagcgcg cctggaagag cgcaccttct
     3181 tctcggagct gatcgggaac gagaccctcc gggccgacct ctggcccgag ccggccgaga
     3241 ccctcgcgct gcgggaccgc gtcagggagc gggagcagcg ggccatcctc ctcgcgacgg
     3301 cggcccgcct ggggcactcc ctcatcgacc tctacatcct cgcggtcaag cgccgcggga
     3361 acctgaaccc tggccgggct gaagaggacg ccgaggagcg ggaagacgca gcggtcgccg
     3421 aggccggcga cgactcgctc atcgacgact tcctccggct cctggagggg cagcggacgc
     3481 tgccgccggg gttcgggtgg ttcggggcgt acgacgagct ctccgccatc ggccggaact
     3541 tccccctcat cgtcgacgtg aacatcccga acatcgccga aatgccgctg gcggcgaccg
     3601 ctgcgcgctt cgggaggctc ctcggggagc agcagccggt ggccgggatg tcggggcaag
     3661 tcaatatgac cctcgtccag cagttccgga tgccgggcta ccccttcgtc ctcgtgagca
     3721 ccgacctcct gcaggaagga gaggacctcc acaccttctg ctccgacgtc ttccactacg
     3781 gcatctcctg gacgccctcg tcgatggagc agcgaatcgg ccgcgtagac cgggtgcggt
     3841 ctcagacgga gcggcggctc agggaggccg atcgaaagcc cgaggggaac gacaagctgc
     3901 aggtctacta cccctacctc tccgacacgg tcgaggtgct ccaggtcgtc cgtgtgctga
     3961 agcggatgga cacgttcctc cgcctgatgc acgaggacct ggccctgccg gagttcgacc
     4021 agaagcggct ctcgatctct cacgagatca tgggcctgcc ccgcccgccc gagcccatcc
     4081 cgacgccgct cgagagcgcg ttcccggtgc gcaaggacgt ccacctcaac gggaagggga
     4141 agaagctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gcgcgttccc
     4261 ggtgcgcaag gacgtccacc tcaacgggaa ggggaagaag ctcgtcgtcg acgccgaaac
     4321 ggagaaggcc gtgcgagagc ggctcgagcg gatgtgcacg ccgcgggtcg gcaagctggc
     4381 cgtcgaatgg caggtgaact ctccgccggg gcagaagctc gggatcgccc gcctcggcac
     4441 gcgccagcag cccttcgtcc tggaggtcca ggggcgcgac cagggccttc tcctgcgatg
     4501 ctcgagccac gtcgggcaga tcggcctgaa ggcgcagcag cagaagctcc tcgacgccgt
     4561 cgccacgaag ggcggccgga tccaggtcca gaaggtcggg aagaaggggg agtggctggt
     4621 gagcctggcc gaggacgtcc ttctcgccgg agaggttcac gacgcggctc gcgcggcctg
     4681 gctcgtcgaa cgggtcgtct gcggggccga tgacctcgag atcgagcttc tcggcgacaa
     4741 ggacgccagc ctcgaagaca tggtcgaagc ccgcaagcac cacggccgtg cccgccacga
     4801 gggagacgcg aatgcctgac ctctccgatc tcctcgcggc aaccgacatg aaggtgaccg
     4861 gccggaaggt gaaggtctcc ttccccaacg gccgctcgca ccgcgtctcc gtttccgaga
     4921 agcccgacgc ctgggagctc cgcggcgtcg tcctgtcggc cgacaccgtt tccgagcagt
     4981 acagcgacga cgactccgat cggctcggtc tcgccctccg ggcctggcag cgcaacggca
     5041 ccgcccgcct ctgtggcttc cgcctcgatt ccgatggcgc cctcgtcggc gaggccacgg
     5101 tgccgaagat cggcctgacc gcggaggagc tgacggagat ggcccgagcc gtcgctcggg
     5161 cctgcgacct cctcgaggcg cacctgacgg gggaggacga ggagtaggcg aggcagggtg
     5221 gccggtcgac agaattcgcc ccgcgtcgtg gtggatacgt ataatgcctc atgggagcat
     5281 gagcatatga gtcatcgtct gcaaatactt gtgcccaagc cgcttgacgc caggatcgtg
     5341 aaagcggcga gccgcaggag gatgtcgaag ggcgcatggg tacggaaggt gctcgaaacg
     5401 gcgctcgacc aggagacgtc gggagaggac ccgctcgctc gcctgtccgc actcggtgcg
     5461 ccgaccgcgg acattcacca gatgctttcg gagatcgagg ccggccggaa atgaccgtct
     5521 tcatcgactc gaacatcccg atgtatgtgg ccgggcgccc tcacccgaat cgcgaaccgt
     5581 cgaggcggtt cctcgagcga atccgggccg gcgaggtgga cggcgtgacg agcaccgagg
     5641 tgctccagga gatcctcttt cggtacgcgg ggctcggcca gctcgagctc gggctccagg
     5701 tctacgacct cttcgtccag atctgtgccc gggtgctgcc ggtcacactg gccgacaccg
     5761 atcgggcgcg agctctcctg acgagcctga cgggagtgag cgttcgcgac acgatccatg
     5821 cctccgtgat gctgacgaac ggcatctcga ggatcgcgac gttcgacgag ggattcgacc
     5881 gggttcccgg cgtacggcgc gcgaagctcg agtgagcgca gcgtcgatga cggccgagtg
     5941 gctggcgggg ccagggtgat cctgcttccc ggagccctcg ttcgccaccc cggccttccc
     6001 tcctggggcc ccggggaggt ccttgttcat ggagtttcac tacc
//
LOCUS       DAOONL010000173         6025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15671_length_6025_cov_17.532496, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000173 DAOONL010000000
VERSION     DAOONL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15671_length_6025_cov_17.532496"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1065
                     /locus_tag="PLP50_15845"
     CDS             88..1065
                     /locus_tag="PLP50_15845"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPA53066.1"
                     /translation="MARPSRTLDDRLADLRALERSPEAPDTEAVREALRSKTGAVVAL
                     AARIVAGRELRGLAAELGPAFTRLCERPVERDPGCRGKVAIAKALRALEEWEDEVFVP
                     GVALVQHEPVWGGREDTAAELRAECAMAFAHSGREDALEVLADLLADPERAARAGAAQ
                     ALGDTGRPDAAALLRYKVRIGDPEPDVLSACLGSILALGPRSSLPFFAGLLEGRDERA
                     EAAALALGSSRLAAALPLLLAYCGEATRDARERTGYLALALLRDDAANDVLLGVVRDG
                     ELPDAVAAARALSTFRDDARVAARLRAAAQGRERAVAEASGVFTPAPRG"
     gene            complement(1227..2588)
                     /locus_tag="PLP50_15850"
     CDS             complement(1227..2588)
                     /locus_tag="PLP50_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53067.1"
                     /translation="MTKARIAGGVVAAALVVLAVAFVVRPRAGEPPAAHDDGLPRPAV
                     VGAGTAAPGEIHPGFLYGRVTTLGGDTYEGRLRWGGAQEAFWGDYFNGRKEANPWLAH
                     VPPDRLPKERRGLSLFGIELGGRRREDDYVRLFMARFGDVARIEARGGDVRVTLRSGA
                     AFDLNRFDASDFDDGVRVWDARRGEVDLDSARIRSVELLPAGPSAAVPSRLFGTVRSR
                     SGEFTGFIQWDREECVGSDTLDGRSDAGEVRLRFDAIRSIARGEPVGSLVTLADGGEV
                     ALSGTNDVSDGNRGVYVDDLRYGRVLVSWEVFERVDFREGGEGPGYGDFPPGGPLSGS
                     VTTRDGRRLAGRLVYDLDESETTETLDAPAAGIDYTIPFGLVASVEPAGEGATPARVT
                     LRSGEELKLERKGDLGARNAGLLVFVEGAAGPEYVSWKEVARIDFDGPKAFYPPIVSR
                     HDR"
     gene            complement(2614..3657)
                     /locus_tag="PLP50_15855"
     CDS             complement(2614..3657)
                     /locus_tag="PLP50_15855"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HPA53068.1"
                     /translation="MRTSRFVVAGALALAAVPALAQIPPLQFPAASPKASVTQVVGMT
                     EVGVRWSRPTVNGRKVWGGLVPYGEVWRTGADENTTLSFSSPVTVGGKTLPAGTYGLH
                     TIPGEKEWTVILSTQAKAWGSYSYDPKEDVVRFVATPREGPHEERLLFTLAEPTDRSV
                     AITLAWEKLRLSFPVEVDTKAVVLASLKEQLRGLPRFGWQAWSGAAAWCVRNDTALEQ
                     AEEWVDRSIRMNRNFTNQSTKADLLEKKGDAKGAAELRAKALALATEAEVNQYGYQLL
                     GQKKVDEAIRAFEKNVKDHPASWNVWDSLAEAQALAGDAKRAIGNYEKALSLTEDEEQ
                     KTRIRGVLAKLKK"
     gene            3975..4901
                     /locus_tag="PLP50_15860"
     CDS             3975..4901
                     /locus_tag="PLP50_15860"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HPA53069.1"
                     /translation="MRPARPSLFPVVALLVASLVPTAARAGNLVRGVRSKLSAGDLAS
                     GQAAVEAHRKATGVDAEYLDAVGWLARGAQMLGRPDLAAAWVAELRREIPAEKPDLLI
                     PLGAAIEVEAKLRLARDGRGAAVSYLEGEAIRAKDVALRSRIRKSLDLLTLEGQPAPP
                     LGLADHVGPAPRALAELKGRPVLLFLFARWCGDCKAEAPALARLSSTYAPKGLALVAA
                     TRLYGPGEDGKPATPAEEKELVEKSWRESYPGLESVPVVLDTETMVTYGASATPTYVL
                     VDRKGVVRLYAPTRLAESELARRIEDLLAETP"
     gene            5233..5634
                     /locus_tag="PLP50_15865"
     CDS             5233..5634
                     /locus_tag="PLP50_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA53070.1"
                     /translation="MAALRRANQLFGAALLLQVAELRRAGDPVERALARTKELELQLA
                     FALPTIDVLLELLNRVPSAHRSRCRKADRGRILLHKDVFHLSCREIARLYALTRSTVH
                     RWVVDCVAPKRPDRPLVAPPPDRSYPVLLRQ"
BASE COUNT          780 a         2354 c         2026 g          865 t
ORIGIN      
        1 ggccgcgcag accgcagcgc tgccacgaaa gggcttcggc cggccgcccc gcgggggacc
       61 accgacccca tccgctacca tcgccgcatg gctcgcccct cgaggacgct ggacgaccgg
      121 ctcgccgacc tccgcgccct ggaacgatca ccggaggcgc cggacaccga ggccgtgcgc
      181 gaggcgctcc gatcgaagac cggagcggtc gtggcgctcg ccgcgcggat cgtcgcggga
      241 cgggagctgc gcggcctcgc ggccgagctg gggccggcgt tcacgcggct gtgtgagcgc
      301 cccgtcgagc gcgacccggg ctgccgcggg aaggtcgcga tcgcgaaggc gctccgcgcg
      361 ctcgaggaat gggaggacga ggtcttcgtc ccgggcgtcg cgctcgtgca gcacgagccg
      421 gtgtggggcg gacgggaaga cacggcggcc gagctccgcg ccgagtgcgc gatggccttc
      481 gcccactcgg ggcgcgagga cgcgctcgag gtccttgccg atctcctcgc cgacccggaa
      541 cgcgcggcgc gcgccggcgc cgcccaggcg ctcggcgaca ccgggcgccc cgacgcggcc
      601 gcgctccttc gctacaaggt gcggatcggg gacccggagc cggacgtcct ctccgcctgc
      661 ctcgggtcga tcctcgcgct cgggccgcgg tcgtccctcc ccttcttcgc aggcctcctc
      721 gagggacgcg acgagagggc cgaggccgcc gcgctcgcgc tcggctcgag ccggctcgcg
      781 gccgcgcttc cgctcctcct cgcgtactgc ggggaggcga cgcgcgacgc gcgcgagcgg
      841 acggggtacc tcgccctggc gctcctgagg gacgacgccg cgaacgacgt cctcctcggc
      901 gtcgtccgcg acggggagct ccccgacgcc gtcgcggcgg cgcgggccct gtcgacgttc
      961 cgcgacgacg cgcgggtggc cgcacgcctc cgcgccgcgg cgcaggggcg agagcgggcc
     1021 gtcgccgagg cgtccggcgt cttcacacct gcgccgcgag ggtgacgaga gctgcggcga
     1081 ggcagccgga ggcacccgag agctccttcg cgacggcctc ggcgcgcgca ccgtgcaggc
     1141 gaggcttctc ccgtcccatc agaaaaggga gggtcgagcg gccggccgcc ggccgccagt
     1201 cttgcgggaa ggcggtgacc ccgtcgtcac cggtcgtgcc gagagacgat cggcgggtag
     1261 aacgccttcg gcccgtcgaa gtcgatccgc gcgacctcct tccaggagac gtactcgggc
     1321 cccgccgcgc cctcgacgaa gacgagcagc ccggcgttcc tcgcgccgag gtcccccttc
     1381 cgctcgagct tcagctcctc gccgctccgg agcgtgaccc gcgcgggcgt cgcgccctct
     1441 cccgcgggct cgacggaagc gacgaggccg aacgggatcg tgtagtcgat tcccgccgcc
     1501 ggagcgtcga gcgtctccgt cgtctcgctc tcgtcgaggt cgtagacgag gcgtccggcc
     1561 aggcggcggc cgtcgcgcgt cgtcacgctc cccgagagcg gcccgcccgg cgggaagtcg
     1621 ccgtaccccg gaccctcgcc gccctcgcgg aagtcgacgc gctcgaagac ctcccaggag
     1681 acgaggaccc gcccgtatcg caggtcgtcg acgtagacgc cgcggttccc gtcgctcacg
     1741 tcgttcgtcc ccgagagcgc gacctcgccg ccgtcggcga gcgtcacgag ggagccgacg
     1801 ggctcccctc gagcgatcga gcggatcgcg tcgaaccgga gccgcacctc gcccgcgtcg
     1861 ctccggccgt cgagcgtgtc cgagccgacg cactcctccc ggtcccactg gatgaacccc
     1921 gtgaactctc cgcttcgcga gcggactgtg ccgaagagcc gggacgggac ggcggccgag
     1981 gggccggccg gaaggagctc caccgagcgg atccgcgccg aatcgaggtc cacctcgccc
     2041 cggcgggcgt cccagacgcg cacgccgtcg tcgaagtcgc tcgcgtcgaa gcgattcagg
     2101 tcgaaggcgg ccccgctccg gagcgtcacc cggacgtcgc caccccgtgc ctcgatcctc
     2161 gcgacgtcgc cgaaacgcgc catgaagagc cggacgtagt cgtcctctct cctccgcccg
     2221 ccgagctcga tcccgaagag cgagagaccc cggcgctcct tcggaagccg atcgggcggc
     2281 acgtgggcga gccacgggtt cgcctccttc cggccgttga agtagtcgcc ccagaacgcc
     2341 tcctgtgccc ctccccagcg cagccgcccc tcgtacgtgt cgccgccgag cgtcgtcacg
     2401 cggccgtaga ggaaacccgg gtggatctcc cccggggcgg ccgtcccggc gccgacgacg
     2461 gccggccgcg gcaggccgtc gtcgtgtgcc gccggtggct cgcccgcccg cgggcggacg
     2521 acgaacgcca cggcgaggac gacgagcgcg gcggccacga cgccgccggc gattcgggcc
     2581 ttcgtcatcc gccgcttccc atcgagacca atgctacttc ttcagcttcg cgagcacccc
     2641 gcggatccgc gtcttctgct cctcgtcctc cgtcagcgag agcgccttct cgtagttgcc
     2701 gatcgccctc ttcgcgtccc cggccaacgc ctgcgcctcg gcgagcgagt cccagacgtt
     2761 ccaggaggcc gggtggtcct tcacgttctt ctcgaaggcc cggatcgcct cgtcgacctt
     2821 cttctgcccg aggagctggt agccgtactg gttcacctcg gcctcggtcg cgagcgccag
     2881 cgccttcgcg cgcagctcgg cggccccctt cgcgtccccc ttcttctcga ggaggtcggc
     2941 cttcgtcgac tggttcgtga agttgcggtt catccggatc gagcggtcga cccactcctc
     3001 ggcctgctcg agggccgtgt cgttccggac gcaccaggcg gcggcgccgc tccatgcctg
     3061 ccacccgaag cgcggcaggc cgcggagctg ctccttcagg ctcgcgagga cgacggcctt
     3121 cgtgtcgacc tcgaccggga aggagagccg gagcttctcc caggcgagcg tgatggcgac
     3181 ggagcggtcc gtcggctcgg cgagcgtgaa gaggagacgc tcttcgtgcg gcccctccct
     3241 cggagtcgcg acgaagcgga ccacgtcctc cttcgggtcg tagctgtagc tcccccacgc
     3301 cttcgcctgc gtgctcagga tgaccgtcca ttccttctcg cccgggatcg tgtggagccc
     3361 gtaggtgccg gccgggagag tcttgccgcc gacggtgacc gggctcgaga acgacagcgt
     3421 cgtgttctcg tcggccccgg tcctccagac ctcgccgtac ggcacgaggc cgccccagac
     3481 ctttcgtccg ttcacggtcg ggcgcgacca gcggacgccg acctccgtca tcccgacgac
     3541 ctgggtgacg gacgccttcg ggctcgcggc cgggaattgc agtggcggga tctgggcgag
     3601 ggccggcacg gcggccaggg ccagggcgcc ggcgacgacg aatcgggacg tgcgcattcg
     3661 gagtcctcct ttcggcaggg caacggcgaa gcccgcatcg aatctcggag ctccgcccga
     3721 gtcctgtcgg tgtctcccgg gaaatacgtc gcggggcacg ccgggtttcc acgccggccc
     3781 cggcgtcacg cgcggcccgg gagcgtccgc aggtcctcgc gttcctgcgc ccctaccggg
     3841 cgggcggcgc cttccgcgcc gaatcgtccc ggcgtttccg agcggttcgg gcgccctggc
     3901 acgccccctg ctcgacggtc ttctcgcagc caatccggcc cgaatcgccg cagtccgtcc
     3961 cgaaggagga cccattgaga cctgcccgac catcgctctt ccccgtcgtc gcgctcctcg
     4021 tcgcctcgct cgtcccgacc gccgctcgcg ccggaaacct cgtccgcggc gtccgatcca
     4081 aactctcggc aggcgacctc gcgagcggcc aggcggccgt cgaggcccac aggaaggcga
     4141 cgggcgtcga cgccgagtac ctcgacgccg tcggctggct cgcccgcggc gcgcagatgc
     4201 tgggccggcc cgatctcgcc gccgcctggg tcgccgagct gcgccgcgag atcccggcgg
     4261 agaagcccga tctcctcatc ccgctcgggg ccgcgatcga ggtcgaggcg aagctccgcc
     4321 tcgcgcggga cgggcgcggc gcggccgtct cgtacctcga gggagaggcg attcgcgcga
     4381 aggacgttgc cctccgctcg cgcattcgga agagcctcga cctcctcacc ctggaggggc
     4441 agccggcgcc gcctctcggt ctcgcggacc acgtcggtcc cgcgccccgg gcgctcgccg
     4501 agctgaaggg ccgccccgtc ctcctcttcc tcttcgcccg atggtgcggt gactgcaagg
     4561 ccgaggcccc cgccctcgca cgcctctctt ccacctacgc gccgaaaggg ctcgccctcg
     4621 tcgcggccac gcggctctac gggccgggag aagacggaaa gcccgccacc cccgccgagg
     4681 agaaggagct cgtcgagaag agctggcgag agtcgtaccc gggcctcgag tcggtccccg
     4741 tcgtcctcga caccgagacg atggtgacct acggcgcctc ggcgaccccg acgtacgtcc
     4801 tcgtcgaccg caagggcgtc gtccgcctct acgccccgac acgtctcgcc gagagcgagc
     4861 tcgcgaggag gatcgaggac ctgctggcgg agacgccctg accagccgac cacggcccgc
     4921 cgcgcgagag accggaattt cgttccgatg gaccgattcc gtggggaggg tcacgtgccc
     4981 gaggatggcc tggatcatcc ggacgtcgga cccgttctcg agaatcagcg tagccatcgc
     5041 gtgccggagg agctgacagg agcccgtccg ctccacgccc gaggccctca cgtaccggct
     5101 gccgttgagg ccgctactct cacccgttgc cccgtctcgt gcccagcgac tccacctgcc
     5161 cctcgccaac cgacctcgag acccgcgccc tcgccgccgt catgggcaag gaggcctctc
     5221 ggagatctct tcatggcggc gctccggcgt gcgaaccagc tcttcggtgc ggcgctcctc
     5281 ctgcaggtcg ccgagcttcg ccgagccggc gatcccgtcg agcgcgccct cgcccggacg
     5341 aaggagctcg agctccagct tgccttcgcg ttgccgacga tcgacgtcct cctcgaactt
     5401 ttgaaccgcg tcccttccgc gcacaggtcc cgctgcagga aggccgatcg cggccgaatc
     5461 ctcctccaca aggacgtctt ccatctctcc tgtcgggaga tcgcccgcct ctacgccctg
     5521 acccgctcca ccgtccaccg ctgggtcgtc gactgcgtgg cgccgaagcg gcccgacagg
     5581 cccttggtcg caccgccgcc tgatcgctct taccccgtcc tgctccggca ataggtgctc
     5641 aacctctcga tggccggctt cggcggctct ctccggatcg cccagaccct cgcgcgcgag
     5701 ggatcctcct ctcacgcagc accgtgaggc gttggacgaa ggagcccgct cccacgcgcg
     5761 gcgcttctct ctccagaacg cccgcgacat cgaccagggc ggatgacgaa gcccctctct
     5821 cccgaccgac ctccgtcgca ccacgccccg tcgaacggat cgtccgggca cgccgcccga
     5881 atcacgtcct catggtcgac gtcaccctcg tcaaggcgct catgggcttc ctccgcttcc
     5941 gcctcgccgt cgtcatcgac gtcttctccc gcttccccct cgcctggcgc ctgttctccg
     6001 acgagccgac ggccgaggag atggc
//
LOCUS       DAOONL010000174         6006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15744_length_6006_cov_18.159805, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000174 DAOONL010000000
VERSION     DAOONL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15744_length_6006_cov_18.159805"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="ccsA"
                     /locus_tag="PLP50_15870"
     CDS             complement(<1..854)
                     /gene="ccsA"
                     /locus_tag="PLP50_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPA53071.1"
                     /translation="MPALGYAPGTLALWFALASGIAAIAAYARALALQKEGGPAYEST
                     LLLGRRLYYAHAAAIVVATLILLWLLLNNDFRLSYVTSYSSRELPFRYLFSTLWAGQE
                     GSFLTWLFFGAVIGPFVVASAKEQEPPVMAVYVLSFLGIVAILVKQSPFRFLAEVPPD
                     GQGLNPLLQDPWMVIHPPVMFAGFASLSVPFAFAIGALWTKRWDGWVVRAMPWALFTF
                     VCLGTAILMGGYWAYKTLGWGGYWAWDPVENTSLVPWLATTALVHGMYLQKTREKHRK
                     INVILAILA"
     gene            complement(854..1261)
                     /locus_tag="PLP50_15875"
     CDS             complement(854..1261)
                     /locus_tag="PLP50_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HPA53072.1"
                     /translation="MKKAYWAGAALIVAFLLLGLTTFSSSMTPYVTFDEALSSPRVCQ
                     VMGGLEKGSSKYDTTTQTLRFNLVDLETKKVLPVTYRDVKPANFEEAVSIVAIGKYQG
                     EAFHAEKLLVKCPSKYQGEEAEKHYGAKVSVKS"
     gene            complement(1258..1419)
                     /locus_tag="PLP50_15880"
     CDS             complement(1258..1419)
                     /locus_tag="PLP50_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HPA53073.1"
                     /translation="MTPDAPTVAVSHGALFYVAAVNIIIWAGLLGYLVHLDRKVRAAI
                     ARQTPEEPS"
     gene            complement(1416..2099)
                     /locus_tag="PLP50_15885"
     CDS             complement(1416..2099)
                     /locus_tag="PLP50_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HPA53074.1"
                     /translation="MQVLGTAGKVVLGLGMTVVLWAAFLYAHPAESFVGESSRIVFFH
                     VPTAWISALAFLLAAIHSGLYLKTRKMERDDAAAAAARLGILFCVLATVTGSLFAKVM
                     WNSYWNWDPRQTSIVLLLLVYGAYLGLRQAIEEPERRATLSAAYALIAFVSVPFLMFA
                     VPRMYASLHPDTVINARGRIEMSPDIKLVFFSSMIAFTALFAWMYALDVKATRLVRRL
                     SERTGGFEA"
     gene            complement(2202..2879)
                     /locus_tag="PLP50_15890"
     CDS             complement(2202..2879)
                     /locus_tag="PLP50_15890"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HPA53075.1"
                     /translation="MILRAAAAVFVKDLRTEWRTRVAINALLLFAFSVLVLVGYAVGP
                     TRLTLEDRPVVNSVLLWIVLFFSAMTGLSRSFVNEEETGTAAALRLSAPPASVFLGKL
                     LGNLALLGVVMAVVVPLFLALMSFGIAAPGLFLAVLALGGVGIAAASTFIAAIVSKTA
                     AKGALFAVLATPLLLPPLVAAVTGTQIAATGPESAAGLDAVRLLFAYDGLMVTASFLL
                     FDAVWRE"
     gene            complement(2876..3499)
                     /locus_tag="PLP50_15895"
     CDS             complement(2876..3499)
                     /locus_tag="PLP50_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA53076.1"
                     /translation="MRLVAENVSRSFGPRRVFGPLSFATEPGRVLGVAGANGSGKTTL
                     VKVLAGLLRPSAGSVRLEDAGRPGSRIGVREAAGAIGWCAPDLSLYGELTPAENLEFF
                     ARVAGRLPTREELEERIASVGLDPRRLRSIRTRFLSTGQRQRLKLAYSVLSSPAVLLL
                     DEPGSNLDEAGHRAVAAIVAEQRGRGSVVLATNDPAELALADERVTL"
     gene            3555..4247
                     /locus_tag="PLP50_15900"
     CDS             3555..4247
                     /locus_tag="PLP50_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA53077.1"
                     /translation="MRALLLGFPLFLLIALWRATLRIRLVGREHRDAIERSGRPVLHA
                     IWHQRMVAGIFRFPYEGVVTMASKSRDGDVIAGFLFWWGYRVVRGSSSRGGSEALAEM
                     VAALKGTTRGAALTPDGPRGPARRCKTGILRLAEAVDAPIMPVGASSTRPRFLRSWDR
                     YLVPMPFSRCAVVFGEGLRRAEGESDEAFLARVDAAIDAMTDEADRLCGVVGAPRERE
                     PKAPAETKEGTA"
     gene            4244..5221
                     /locus_tag="PLP50_15905"
     CDS             4244..5221
                     /locus_tag="PLP50_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPA53078.1"
                     /translation="MTTLPVNLDDVKAAAARIASHVHRTPVLTSRTADAACGARLFFK
                     CETFQRVGAFKARGAFSRLTLLSREEMRRGVVAFSSGNHAQAVALAARDLGVPATIVM
                     PDDAPALKLAATRGYGAEVILYDRRNEDREAIAGRLVQERGLTLVPPFDDEAVIAGQG
                     TAALELLAEVPDLDVLVTPCGGAGLLAGTAVAGTALRPGIRVFGVEPEAGDDVARSLR
                     EGRRVAIPVPETIADSLQTTRVGERNFAILQALVEGVLTVSDLELRRAMAFLFSRMKL
                     VVEPGGSAAAAALHAGRVPAVAGRRVGVVLSGGNVDAARFGELVAGLPA"
     gene            complement(5225..5815)
                     /locus_tag="PLP50_15910"
     CDS             complement(5225..5815)
                     /locus_tag="PLP50_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA53079.1"
                     /translation="MRPSFPSAALLGLAAATLAAPAAAQSWSDPGIGFGARLGLTQAR
                     SAGSPSGGAGVQVRYRLTGSLGLEGSLGVRRESLGDDTGPLLRLTEVPVTGTGQLFFF
                     PSRRLQPYLLAGAGLHVVRAEPEGRNPGSDTKTEALFAVHAGAGIDVRPSRTSAVHLD
                     ARWTFLEPTSITDLEKAGFDVAPGYWAIAVGVTFSR"
BASE COUNT          938 a         1959 c         2349 g          760 t
ORIGIN      
        1 gcgaggatcg cgaggatgac gttgatcttc cggtgcttct cgcgcgtctt ctgcaggtac
       61 atcccgtgga cgagggccgt cgtcgcgagc cacgggacga gggaggtgtt ctcgaccggg
      121 tcccaggccc agtagccgcc ccagccgagc gtcttgtagg cccagtagcc tcccatcagg
      181 atcgccgtcc cgaggcagac gaacgtgaag agcgcccacg gcatcgcccg gacgacccag
      241 ccgtcccagc gcttcgtcca gagcgccccg atggcgaacg cgaacgggac ggagagcgag
      301 gcgaagccgg cgaacatgac gggcgggtgg atgaccatcc acgggtcctg aaggagcggg
      361 ttcagcccct gcccgtcggg cgggacctcg gcgaggaacc ggaacggcga ctgcttgacg
      421 aggatcgcga cgatgccgag gaaggagagg acgtagaccg ccatcacggg cggctcctgc
      481 tccttcgcgc tcgccacgac gaacgggccg atgaccgcgc cgaagaagag ccatgtcagg
      541 aacgaccctt cctggcccgc ccagagcgtc gagaagagat agcggaacgg gagctcgcgc
      601 gaggagtacg acgtgacgta ggagagccgg aagtcgttgt tcaggaggag ccagagcagg
      661 atcagcgtcg cgacgacgat cgcggccgca tgcgcgtagt agaggcgccg cccgaggagg
      721 agggtcgact cgtacgccgg gccgccctcc ttctggagcg cgagggcgcg cgcgtaggcg
      781 gcgatcgccg cgatcccgga cgcgagcgcg aaccagagcg cgagcgtccc cggggcgtag
      841 ccgagggccg gcatcaggac ttcaccgaga ccttggcgcc gtagtgcttc tcggcctcct
      901 cgccctggta cttcgagggg cacttcacga ggagcttctc ggcgtggaac gcctcgccct
      961 ggtacttccc gatggcgacg atcgagacgg cctcctcgaa gttggccggc ttgacgtcgc
     1021 ggtaggtgac cgggaggacc ttcttcgtct cgaggtcgac gaggttgaag cggagcgtct
     1081 gggtcgtcgt gtcgtacttc gaggagccct tctcgagccc gcccatcacc tggcagacgc
     1141 ggggcgagga gagcgcctcg tcgaacgtga cgtagggggt catcgaggag gagaaggtcg
     1201 tcaggccgag gaggaggaac gcgacgatga gggcggcgcc ggcccagtag gctttcttca
     1261 cgacggctcc tccggggtct gccgcgcgat tgcggcgcgg accttgcggt cgaggtggac
     1321 gaggtagccg aggagccccg cccagatgat gatgttcacg gcggcgacgt agaagagcgc
     1381 gccgtgcgag acggcgacgg tcggggcgtc gggcgtcatg cttcgaaccc tccggtcctc
     1441 tccgagaggc gccggacgag ccgggtggcc ttcacgtcga gggcgtacat ccaggcgaag
     1501 agcgcggtga acgcgatcat cgaggagaag aagacgagct tgatgtcggg ggacatctcg
     1561 attcggcccc gcgcgttgat cacggtgtcg gggtggagcg aggcgtacat gcgggggacg
     1621 gcgaacatga ggaacgggac ggagacgaac gcgatcaggg cgtaggccgc cgagagcgtc
     1681 gcccgccgct ccggctcctc gatcgcctgg cggaggccga ggtaggcccc gtagacgagg
     1741 aggaggagga cgatcgaggt ctgccgtggg tcccagttcc agtaggagtt ccacatcacc
     1801 ttcgcgaaga gcgagccggt gacggtggcc aggacgcaga agaggatccc gaggcgcgcg
     1861 gcggccgccg ccgcgtcgtc gcgctccatc ttccgggtct tcaggtagag gccggagtgg
     1921 atcgccgcca ggaggaaggc cagggcggag atccaggccg tcgggacgtg gaagaagacg
     1981 atgcgggaag actcgcccac gaagctctcc gcggggtggg cgtagaggaa ggcggcccag
     2041 aggacgaccg tcatcccgag gcccaggacg accttcccgg cggtgccgag aacctgcatc
     2101 ggccgggatg atacgggagc ggagggtgat accggacctt ttcgggactg gattccgaac
     2161 ccccgccgaa cgagcgcttg attcgcctcg gggaggctcg gctactcgcg ccagacggcg
     2221 tcgaagagga ggaacgaggc ggtcaccatc aggccgtcgt aggcgaagag gaggcggacg
     2281 gcgtcgagcc cggcggcgga ctccggcccg gtggcggcga tctgggtccc ggtgacggcc
     2341 gcgacgaggg gagggaggag gagcggcgtg gcgaggacgg cgaagagcgc ccccttcgcc
     2401 gcggtcttgg agacgatcgc cgcgatgaac gtggaggcgg cggcgatgcc gacgccgccg
     2461 agagcgagga ccgcgaggaa gagaccgggg gcggcgatac cgaaggacat gagggccagg
     2521 aagagcggga cgacgacggc catgacgacg ccgaggaggg cgaggtttcc gaggagcttc
     2581 ccgaggaaga cggacgcggg cggggccgag aggcggagcg ccgcggccgt gccggtctcc
     2641 tcctcgttca cgaacgagcg cgagagcccc gtcatcgccg agaagaagag gacgatccag
     2701 aggaggacgg agttgacgac gggacggtcc tcgagcgtga ggcgcgtcgg gccgacggcg
     2761 tagccgacga ggacgaggac cgagaacgcg aagaggagga gcgcgttgat cgcgacgcgc
     2821 gtccgccact cggtccgaag gtccttcacg aagacggccg ccgccgcacg gaggatcaca
     2881 gcgtcaccct ctcgtcggcg agggcgagct cggccgggtc gttcgtcgcc aggacgacgc
     2941 tgccgcggcc ccgctgctcg gcgacgatcg ccgcgacggc gcggtgtccc gcctcgtcga
     3001 ggttcgagcc gggctcgtcg aggaggagga ccgcgggaga ggagaggacc gagtaggcga
     3061 gcttgaggcg ctgcctctgt cccgtggaga ggaagcgcgt ccggatcgag cggaggcgcc
     3121 tcgggtcgag gccgacggaa gcgatgcgct cctcgagctc ctcccgcgtc ggcagccgcc
     3181 ctgcgacgcg ggcgaagaac tcgaggttct cggccggggt cagctcgccg tagagggaga
     3241 ggtccggcgc gcaccagccg atcgcgcccg ccgcctcgcg gacgccgatc cgcgacccgg
     3301 gccggccggc gtcctccagc cggacgctcc cggcggaagg gcggaggagg ccggcgagga
     3361 ccttgacgag cgtcgtcttg cccgagccgt tcgcgccggc gacgccgagg accctccccg
     3421 gctccgtcgc gaacgagagc gggccgaaga cgcggcgcgg cccgaacgag cgggagacgt
     3481 tctcggcgac gagacgcacg ggcggggagg atacagggcg ggcggaggga gcgcgcgtcg
     3541 tggagaatcg gccggtgcgc gccctcctcc tcggcttccc gctcttcctc ctgatcgccc
     3601 tctggagggc gacgctccgg atccgcctcg tcggccgcga gcaccgcgac gcgatcgagc
     3661 gttcggggcg cccggtcctc cacgcgatct ggcaccagcg gatggtcgcg gggatcttcc
     3721 ggttcccgta cgagggcgtc gtgacgatgg cctcgaagag ccgcgacggc gacgtcatcg
     3781 ccggtttcct cttctggtgg ggctatcgcg tcgtccgcgg ctcgtcgtcg cgcggcggga
     3841 gcgaggcgct cgccgagatg gtcgcggcgc tgaaggggac gacgcgcggg gcggcgctga
     3901 cccccgacgg cccgcgcgga ccggcgcgtc gctgcaagac cgggatcctt cgcctggccg
     3961 aggccgtgga cgccccgatc atgccggtcg gcgcgtcgtc gacgcgcccg aggttccttc
     4021 gctcctggga ccgctacctc gttccgatgc cgttctcgcg ctgcgcggtc gtcttcggcg
     4081 aagggctccg gcgggcggag ggggagagcg acgaggcgtt cctcgcgcgc gtcgacgcgg
     4141 cgatcgacgc gatgacggac gaggccgacc ggctctgcgg cgtcgtcggg gcgccgcggg
     4201 aacgggagcc gaaggcaccc gcggagacga aggaggggac ggcgtgacga cgctcccggt
     4261 caatctcgac gacgtgaagg ccgccgcggc gcgcatcgcg tctcacgtcc accggacccc
     4321 ggtcctgacg tcacggacgg ccgacgccgc ctgcggggca cggctcttct tcaagtgcga
     4381 gacgttccag cgagtcgggg cgttcaaggc gcgcggcgcg ttctcgcgcc tgacgctcct
     4441 ttcccgggag gagatgcggc gcggggtcgt cgcgttctcg tccgggaatc acgcccaggc
     4501 cgtcgcgctc gccgcgcgcg acctcggcgt cccggcgacg atcgtcatgc ccgacgacgc
     4561 cccggccctc aagctcgctg cgacgcgcgg ctacggcgcc gaggtgatcc tctacgaccg
     4621 acggaacgag gaccgcgagg cgatcgccgg gcggctcgtc caggagcgag ggctcaccct
     4681 cgtccccccg ttcgacgacg aggcggtcat cgccgggcag gggacggcgg cgctcgagct
     4741 cctcgcggag gtgcccgacc tcgacgtgct cgtcacgccc tgcggcggcg ccgggctcct
     4801 cgccgggacc gcggtcgccg ggacggcgct tcggcccggc atccgcgtct tcggagtcga
     4861 gccggaggcg ggcgacgacg tcgcgcgctc gctccgggag ggacggcggg tcgcaatccc
     4921 ggtcccggag acgatcgccg actcgctcca gacgacgcgg gtcggcgagc ggaacttcgc
     4981 gatcctgcag gcactcgtcg aaggggtcct caccgtgagc gacctcgagc tccgccgtgc
     5041 gatggcgttc ctcttctcac ggatgaagct cgtcgtcgaa ccggggggct cggccgcggc
     5101 ggcggcgctc cacgcgggaa gggtgcccgc cgtcgcgggc cgccgggtcg gcgtcgtcct
     5161 ctcgggcggg aacgtcgacg ccgcgcgctt cggcgagctg gtggccggcc ttcccgcctg
     5221 acgttcagcg ggagaacgtg acgccgaccg cgatggccca gtaaccgggc gccacgtcga
     5281 agccggcctt ctcgaggtcg gtgatgctcg tcggctcgag gaaggtccag cgcgcgtcga
     5341 ggtggacggc gctcgtccgc gaggggcgga cgtcgatccc cgccccggcg tggacggcga
     5401 agagggcctc cgtcttcgtg tccgagcccg ggttccgccc ttccggctcg gcgcggacga
     5461 cgtggaggcc tgcgccggcg aggaggtagg gctggagacg ccggctcggg aagaagaaga
     5521 gctggccggt gccggtgacg ggcacttccg tcaggcggag gagcggcccc gtgtcgtcgc
     5581 cgagcgactc acggcggaca ccgagggagc cctcgaggcc gagagagccg gtcaggcggt
     5641 agcggacctg gaccccggcg cccccggacg gcgagccggc ggagcgggcc tgcgtcagcc
     5701 cgagacgggc tccgaagccg atcccggggt cggaccacga ctgcgccgcg gcgggcgccg
     5761 cgagggtcgc tgcggccagg ccgaggagcg ctgcggacgg aaaggaaggc ctcacgcggt
     5821 ccagatggta acggcacggg ccgtccgagg ggcgatcgtc gataatcgcg ggacgtgagc
     5881 cgaaggaaga gctggcagga ggacctgatc ccggttgcgg tcgcggtgct cgcccaggtg
     5941 gcgatgctcc ggttcgtcga ttgggcgaac gcgaacctcg ccacccagcc gatcgcgcgg
     6001 cagtgg
//
LOCUS       DAOONL010000175         5969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15883_length_5969_cov_18.422218, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000175 DAOONL010000000
VERSION     DAOONL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15883_length_5969_cov_18.422218"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..901)
                     /locus_tag="PLP50_15915"
     CDS             complement(32..901)
                     /locus_tag="PLP50_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPA53080.1"
                     /translation="MTKKPALGKGLSALISTNRPAPAEPRPGSSVQTIDLDHLEANRR
                     QPRRRFDDDGIEELARSIKESGILQPILVTRDGERFRILAGERRARAARLAGLSRVPV
                     LIREGLEDRDHLLLALVENLQRRDLTAVEEAEGYQHLKDSFGMTQEEIAERVGKDRTT
                     VANAMRLLKLPAEIRTQVEDGLLTAGHVRPLLALSSAADQETLAREILRRGLSVRAAE
                     SRVQEFLAGGPKKPAAPEGDPDTRDAERRLQRALGTKVEIRRRKKGGEVRLSFYSEEQ
                     LIGLFERLMGGEG"
     gene            complement(898..1683)
                     /locus_tag="PLP50_15920"
     CDS             complement(898..1683)
                     /locus_tag="PLP50_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA53081.1"
                     /translation="MKRLAVANQKGGVGKTTTAISLAAALSVLEKRVLLVDFDPQGNT
                     TGGLGVEKGSLERTVYDWILGNAALSEVARPTELAGLKLIPASRDLVGAEIELVGVEE
                     REYLLARKLDGLADEFDYVFIDCPPSLGLLTLNALTAADGVLVPIQCEYYALEGVSEL
                     VSTIERVREGLNPTLAIAGVVATMLDERLNLSRQVLEEIRKYFGEVFLEPPVPRNVRL
                     GEAPSFGKPIFLYDIRSRGAEAYLAIAREILRRDLAGGNGGQP"
     gene            complement(1811..2461)
                     /locus_tag="PLP50_15925"
     CDS             complement(1811..2461)
                     /locus_tag="PLP50_15925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53082.1"
                     /translation="MTGPVTAVLGALERAGLPIGASAAERLATFLGLLLRRNEAVNLV
                     SRKEATVETLLERHVLDALGGLRNLPDPAGRPLRLLDIGSGGGFPAIPLLLARGDLEG
                     VLVESTGKKARFLEEAIEALELTARVVNARFPDPAIDRMKNALPCDLLTSRAVADAGG
                     ILRQARPVMRAGAVALFWTTEPLAGELRRNVPRAVVSFSKSPGAERRGIARVECFT"
     gene            complement(2458..4356)
                     /gene="mnmG"
                     /locus_tag="PLP50_15930"
     CDS             complement(2458..4356)
                     /gene="mnmG"
                     /locus_tag="PLP50_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPA53083.1"
                     /translation="MRARYEVVVVGGGHAGAEAAAAAARLGRRTLLLTRERAALGRMS
                     CNPAIGGLAKGQVVREIDALGGLMAHAADRTGIQFKVLNASRGPAVQGLRCQSDRIAY
                     AAEVSELLSRQPSLTIAEGTAAGFLVRGGRLAGLRLEDGSELLCSSVVVTSGTFLRGL
                     VHIGERREEAGRWGEAPCSGLSESLRALGLRLGRMKTGTPPRVHRESIDRSKLLRSAG
                     DGRPRRFSLRSRIEPFPLLPQVDCWLSHTNERTHDLIRANLGRSPLYSGRIVGRGPRY
                     CPSIEDKVVRFPDRERHTIFIEPEGHGTDWTYLGGLSMSLPVGLQLEVVRSLPGLERA
                     EILRPAYAVEYDVVFPEQLEDSLETRALPGLFLAGQVNGTSGYEEAAGQGLVAGANAA
                     LVAGGRIGPPFTLGRHEAYIGVMIDDLVTLGADEPYRLLTSRAEHRLLLGAESAYARL
                     VPRAIAAGLLPEKEAEPLLEREARQAAAREALGQLRVVPDRETVATLDRLSVTLAEES
                     TGAGLLRRPDAPESLRGWLEERLPGLLGELDEEEWSRLVNDLRYEGFLRRERESLERV
                     RRAEEKRIPAGFRYAGIPGLSAEAAEKLERHRPRTLGQAARIPGVTAAAVTLLLARLA
                     VRGGEAAA"
     gene            complement(4353..5687)
                     /locus_tag="PLP50_15935"
     CDS             complement(4353..5687)
                     /locus_tag="PLP50_15935"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HPA53084.1"
                     /translation="MAPLVADGAGVADVLVARATPPGSGALAIVRLSGPPGTTLGIAR
                     ALAPRLPARPEARRAYLSPFRDEEGALLDEGLALYFAAPRSATGEEVVELQAHGAPAI
                     AEGLVAAAVALGARRARPGEFTRRALANGRIDLARAEGIGRLAGAASRGEARRALGLV
                     RGELSLRVEALREELLTVLADLEAGLDFAEDVEEDGEAAALARARRLHEELAKLGRVV
                     GVGRAEAGLPTVVLVGAPNAGKSTLFNALVGEDRALVTETPGTTRDAVSEVVEIGGER
                     VRLVDTAGLREAEELVERLGVETARRAAGGADLLLLACAPDAPGHEAEGLPEGVPVLR
                     VATKADLGGAGAEGALAVSARTGRGLDELRREIAGRLGAEPEPGAWGALPRQGEAVRR
                     AAEALQALSAGLPGELAAARVRAALHALGEITGETATEELLERIFERFCVGK"
     gene            complement(5725..>5969)
                     /locus_tag="PLP50_15940"
     CDS             complement(5725..>5969)
                     /locus_tag="PLP50_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPA53085.1"
                     /translation="TPILMTLTMWLQQQMMPATGDATTRKVMGFLPLVFGFMFKDMPS
                     GLVLYWLVQNVLSIAQQLLLERYTDLGMRPKAAKKA"
BASE COUNT          819 a         2316 c         2002 g          832 t
ORIGIN      
        1 ccgttcagga cgcccccctt cttcccgccg atcacccttc ccctcccatg aggcgctcga
       61 agaggccgat cagctgctcc tccgagtaga aggagaggcg gacctctccc cctttcttcc
      121 ggcgacggat ctcgaccttc gttccgaggg cccgctgtag ccggcgctcg gcgtcccggg
      181 tgtccgggtc gccctcgggg gcggccggct tcttcggccc tccggcaagg aactcctgga
      241 cgcgcgactc ggccgcgcgg accgataggc cgcgccggag gatctcgcgg gccagcgtct
      301 cctggtccgc ggccgaggag agcgcgagga gcggccggac atggcccgcg gtgaggagac
      361 catcctccac ctgggtccgg atttcggccg ggagcttcag gaggcgcatc gcgttcgcga
      421 cggtcgtcct gtccttcccg acccgctccg cgatttcctc ctgggtcatc ccgaagctgt
      481 ccttcaggtg ctggtatccc tccgcctcct cgaccgctgt caggtcgcgg cgctggaggt
      541 tctccaccag ggccaggagg aggtggtctc ggtcctcgag cccttcccgg atgaggaccg
      601 gcactcgcga gagcccggcc aggcgggcgg cccgggcccg gcgctcgccg gcgaggatcc
      661 ggaaacgctc cccgtcccgg gtgacgagga tcggctggag aatccccgat tccttgatcg
      721 accgggccag ctcctcgatg ccgtcgtcgt cgaaccgtcg ccggggctgg cgacggttcg
      781 cctcgaggtg gtcgaggtcg atcgtctgga ccgagctccc ggggcggggc tcggccgggg
      841 ccgggcggtt cgtggaaatg agggccgaga ggccctttcc gagcgcgggc ttcttcgtca
      901 cggctgccct ccgttcccgc cggccaggtc tcgccgcagg atctctcgcg cgatcgcgag
      961 gtaggcctcg gctccgcgcg agcggatgtc gtacaggaag atcggcttcc cgaacgaggg
     1021 ggcctccccg agccggacgt tcctcgggac cggcggctcg aggaagacct ccccgaagta
     1081 cttccgaatc tcttcaagga cctggcggga gaggttgagc ctctcgtcca gcatcgtcgc
     1141 gacgacgcca gcgatcgcga gggtcggatt gagcccctcg cggacccgct cgatcgtcga
     1201 gacgagctcg gagaccccct cgagggcgta gtactcgcac tgaatcggaa cgaggacgcc
     1261 gtcagccgcc gtcagggcgt tcagcgtcag gagaccgagt gaaggcgggc agtcgatgaa
     1321 gacgtagtcg aactcgtctg cgagcccatc cagctttcgg gcgaggaggt actcccgctc
     1381 ctccacgccg acgagctcga tctccgcgcc gacgaggtcg cgggaagccg ggatgagctt
     1441 caacccggcg agctcggtcg gccgtgccac ctcgctcagg gcggcgttcc cgagaatcca
     1501 gtcgtagacc gttctctcga ggctcccctt ctcgacgccg agcccgccgg tcgtgttccc
     1561 ctgcgggtca aagtcgacga gaaggacccg cttctcgagg acggagagcg ccgcggcgag
     1621 gctgatcgcc gtcgtcgtct ttccgacgcc ccctttctgg ttcgcgaccg ccaaccgttt
     1681 cacgtggaac tcccccttcg ccggtacctg cggccggccc ctccacggca cttcccggcg
     1741 cccggatcgc gcggccgcgc cccgaggatc gggccgatcc ggtcgctgtg gatctccctg
     1801 tggaaatgtt tcacgtgaaa cactccaccc gggcgatccc ccgccgctcc gcgccgggac
     1861 tcttcgagaa tgagacgacg gccctcggga cgttgcgacg aagctctccg gcaagcggct
     1921 ccgtcgtcca gaagagcgcg acggcgcccg cccgcatcac aggacgggcc tgccggagga
     1981 tccctccggc gtcggcgacg gcgcggctcg tcaacaggtc gcagggaagg gcgttcttca
     2041 tccgatcgat cgcgggatcg gggaatctag cgttcacgac acgggccgtc aattcgagcg
     2101 cctcgatcgc ctcctccagg aaccgggcct tcttccccgt cgattcgacg aggacgccct
     2161 ccaggtctcc gcgcgcgagg agcagcggaa tcgccgggaa gccccctccc gacccgatgt
     2221 ccaggagccg caggggccgc ccggccggat cgggcagatt ccgcaggccg cccagggcgt
     2281 ccagaacgtg ccgctcgagg agcgtttcga ccgtcgcctc cttccgcgag actaggttga
     2341 cggcctcgtt gcggcgcagg agaaggccga ggaaggtggc gagcctctcg gcggcggagg
     2401 cgccgatcgg gagccccgcc ctctcgagcg ccccgaggac cgccgtcacc ggacccgtca
     2461 cgcggccgcc tcaccgcccc ggaccgccag gcgggcgagg aggagcgtca ccgccgcggc
     2521 ggtgacgccc ggaatccgtg cggcctgccc gagcgtccgg ggccggtgcc gctccagctt
     2581 ctccgccgcc tccgccgaga gcccgggaat cccggcgtag cgaaacccgg ccgggatcct
     2641 cttctcttcg gcccggcgga cccgctcgag cgactcccgc tcccgtcgca ggaacccctc
     2701 gtaccggagg tcgttcacca gcctcgacca ctcctcctcg tcgagctctc cgagcagacc
     2761 cgggagccgc tcctcgagcc agccgcggag gctctctggc gcgtccggtc gccggagcag
     2821 gcccgctccg gtcgattcct ccgcgagcgt gacggagagg cggtcgagcg tggcgacggt
     2881 ctcccgatcg gggacgacgc gtagctggcc cagcgcttcg cgggccgccg cctgccgggc
     2941 ctcccgctcg aggagcggct cggcctcctt ctcggggaga agcccggcgg cgatcgcccg
     3001 cggtaccagt cgggcgtacg ccgattcggc accgaggagg agccggtgct ccgcccgcga
     3061 agtgagaagt cgatagggtt cgtccgcgcc gagagtcacg aggtcgtcga tcatcacacc
     3121 gatataggcc tcgtgccgcc ccagcgtgaa cggcggcccg atccggcccc cggcgacgag
     3181 cgccgcgttc gccccggcga cgagcccctg tcccgccgcc tcctcgtacc cggaggtccc
     3241 gttcacctgt ccagcgagga agaggcccgg aagcgcacgc gtctcgagcg agtcctcgag
     3301 ctgctccggg aagacgacgt cgtactcgac ggcgtacgcc ggccgcagga tctccgcccg
     3361 ctcgagcccc ggcaggctcc ggacgacttc gagctggagc ccgaccggaa gggacatcga
     3421 cagtccgccg agataggtcc aatccgtccc gtgtccttcc ggctcgatga agatcgtgtg
     3481 ccgctctcga tccggaaaac ggacgacctt gtcctcgatg gaagggcagt acctcggccc
     3541 gcgccccacg atccgaccgg aatagagcgg cgagcgtccg aggttcgccc ggatcaggtc
     3601 gtgcgtccgt tcgttcgtgt ggctcagcca gcagtcgacc tgcggcagga gcgggaacgg
     3661 ctcgatccgg cttcgaagcg agaaccggcg cggccgcccg tcccccgcgg agcggagcaa
     3721 ttttgatctg tcgattgatt cacgatgcac cctggggggg gtccccgtct tcatccgccc
     3781 caggcggagg ccgagcgccc ggagactctc ggatagcccg gagcagggcg cctcgcccca
     3841 ccgccccgcc tcctcccgac gctccccgat gtgaacgagc ccgcgcagga acgtcccgct
     3901 cgtcacgacg acggaagagc agagcagctc cgacccgtcc tcgagccgga ggccggccag
     3961 gcgcccgccc cggacaagga aaccggcggc cgtcccctcc gcgatcgtca ggctcggctg
     4021 acgagagaga agctcggaga cctcggccgc gtaggcgatc cgatccgact ggcaccgaag
     4081 gccctggacc gctggtccgc gggacgcgtt gagcaccttg aactggatcc ccgtgcggtc
     4141 cgccgcatgg gccatcagtc cgccgagcgc gtcgatctcg cggacgacct gcccttttgc
     4201 gaggcccccg atcgccgggt tgcagctcat ccgcccgagg gcggcccgct cgcgcgtcag
     4261 gaggagcgtc ctcctcccga ggcgggcggc cgccgcggcc gcttccgccc cggcgtgtcc
     4321 gcccccgacg acgacgacct cgtatcgcgc tctcatttcc cgacgcagaa cctctcgaag
     4381 atcctctcga ggagctcctc cgtcgccgtc tctccggtga tctcgccgag cgcgtggagc
     4441 gcggcgcgca cccgggccgc cgcgagctct cccgggagcc cggccgaaag cgcctgaagc
     4501 gcttccgccg cccggcgcac cgcctccccc tgccgcggaa gcgcccccca ggctcccggc
     4561 tccggctcgg cgccgagccg gccggcgatc tctcgccgga gctcgtccag cccgcggccc
     4621 gtccgcgcgc tcacggccag ggcgccctcc gcgcccgccc ccccgaggtc ggccttcgtc
     4681 gcgacccgca gcacggggac gccttcgggc agcccttcgg cctcgtgccc gggggcatcg
     4741 ggcgcgcacg ccaggaggag caggtccgcg cccccggccg cacgccgggc cgtctcgacg
     4801 ccgagccgct ccaccagctc ctccgcctcc cgaagccccg cggtgtcgac gagacgaacc
     4861 cgctcgcccc cgatctcgac gacctccgaa acggcgtcgc gcgtcgtccc cggcgtctcc
     4921 gtcacgagcg cgcgatcctc gccgacgagc gcgttgaaga gcgtcgactt cccggcattc
     4981 ggcgcaccga ccaggaccac agtcggcagg ccggcctcgg cccggcccac gccgacgacc
     5041 cgccccagct tcgccagctc ctcgtgcagc cgccgagcgc gcgcgagcgc cgcggcctcg
     5101 ccgtcctcct ccacgtcctc cgcgaagtcg agccccgcct ccaggtcggc cagcacggtg
     5161 aggagctcct cccggagcgc ctccacccgg agcgagagct ctccccgtac cagcccgagc
     5221 gctcgccgcg cttccccccg gctcgccgct cccgccagcc gaccgatccc ctccgcgcgc
     5281 gcgaggtcga ttcgcccgtt cgcgagcgcc cgccgcgtga actcgcccgg ccgcgcgcgc
     5341 cgggccccga gagccacggc ggcggccacg agcccctcgg cgatcgccgg ggcgccgtgc
     5401 gcctgaagct cgacgacctc ttcccccgtc gcagaccgcg gcgccgcgaa gtagagcgcg
     5461 agcccctcgt ccaggagtgc gccctcctcg tcccggaatg gggagaggta cgcccgtcgc
     5521 gcctccggcc gcgccggcag ccgcggcgcc agcgcccgcg cgatcccgag cgtcgtgccg
     5581 ggcgggccgc tcagccggac gatcgcgagc gcgcccgagc cgggcggcgt cgcgcgagcc
     5641 acgagcacgt ccgcgacccc ggcgccgtcc gcgacgagag gggccaccgt tttggcccct
     5701 ccgtcgcgcg cccgcccgcg cgcgtcacgc cttcttcgcc gccttcgggc gcatcccgag
     5761 gtccgtatag cgctcgagca ggagctgctg cgcgatggag aggacgttct gcacgagcca
     5821 gtagaggacg aggcccgacg gcatgtcctt gaacatgaac ccgaagacga gcgggaggaa
     5881 ccccatcacc ttcctcgtcg tcgcgtcgcc cgtggccggc atcatctgct gctgcagcca
     5941 catcgtcagc gtcatcagga tcggcgtga
//
LOCUS       DAOONL010000176         5947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15972_length_5947_cov_10.085709, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000176 DAOONL010000000
VERSION     DAOONL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_5947_cov_10.085709"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..834)
                     /locus_tag="PLP50_15945"
     CDS             complement(7..834)
                     /locus_tag="PLP50_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53086.1"
                     /translation="MGGGGQGGPIDGMTNGSEDLVIPAGVRGEGRFSSFFVTDLWIKA
                     TGTGTATLYFHAADSTSTAPAATATVNLTQPITYLPDVLQANFGLAAGFGAIRVAASI
                     PISATVRVYNQTGAGAYGLAFMGMPSRMGMTSMPMGGSYGMDEYAMYMLGLLPQPGNR
                     VNVNVVSTTSTGATGVVEIVDADGSTPTGTGAKSLPFTLAGYSSHQFNDVLLNVHSKF
                     PTGDAGLQVRVRMNQGGPGMVMAYAVVNDNTTNDGYVVMGSMMNGGRGMGGGMMGGQ"
     gene            complement(1016..1345)
                     /locus_tag="PLP50_15950"
     CDS             complement(1016..1345)
                     /locus_tag="PLP50_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HPA53087.1"
                     /translation="MSLVAALVSAIGASLCCIGPPAVGMLGLAGAGSFALFHEYRPYL
                     AIGTIVLLWASVGLTYRTSSAACCATGTDSRPWNPDPRDRRWRRLLWIVVALTLAFLT
                     IPWWFSP"
     gene            complement(1769..2257)
                     /locus_tag="PLP50_15955"
     CDS             complement(1769..2257)
                     /locus_tag="PLP50_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPA53088.1"
                     /translation="MARTFRGWATPEAARRVPNPVPIGPEVLARARAHFADHCASCHG
                     NDGSGQTPVGRRLYPRAPDMRSGETQRLTDGELFSIIRNGIRFTGMPAWGNGTPPEDV
                     GTWELVHFIRHLPKLTPAEIREMESLNPKSPAEYEKARQIEAFLSGSGSSDASLQKRS
                     AH"
     gene            complement(2415..2828)
                     /locus_tag="PLP50_15960"
     CDS             complement(2415..2828)
                     /locus_tag="PLP50_15960"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPA53089.1"
                     /translation="MRKALSVLAALALTLSTGVASAHDGHAHGKEAILGTVAQVHGDH
                     LEVADPDGKTVSVTLTSATSYRRGDSEAKKTDLKPGVRVAVETAEGKSGLEARVVRIG
                     AEENAVWTCPMHPEVKNAGPGKCPKCGMFLEKKKA"
     gene            2982..3476
                     /locus_tag="PLP50_15965"
     CDS             2982..3476
                     /locus_tag="PLP50_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA53090.1"
                     /translation="MAGTSPRGTLLLVEDDASFAFCAAEFLSRRGFEVAHARDLDSAL
                     VRLAAQRWAAVVTDLDLTGRQTRDGLEVVATAARFEPRPVIFVWTSSSATEVRQEAFR
                     LGADLVVEKGSLWDLEARLRVCLAERPSRVASWPEDVESVPPPGLTAGSEKTPESASA
                     GGRR"
     gene            complement(3648..4052)
                     /locus_tag="PLP50_15970"
     CDS             complement(3648..4052)
                     /locus_tag="PLP50_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53091.1"
                     /translation="MLNHAAHKVQRLAFAALLAAPLTLLPLVADAQQEGGAYPMFADE
                     IARLPEVHLARAEEYERKAASYRKEAAKQRKLLGDSITGNREPERADTDPMILEMRRH
                     YEAYIEKVEAVALEAERFSTFHRKQWETLRGK"
     gene            complement(4142..4426)
                     /locus_tag="PLP50_15975"
     CDS             complement(4142..4426)
                     /locus_tag="PLP50_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53092.1"
                     /translation="MKVAVTPKDHLDRAVEYRKKAAAYREEANRHREMILALKKRLPP
                     DTRPGNYEPPELEKLRGHCNGYIKDAEALAAKAEKLAEYHEMRAAELNGQ"
     gene            5152..5742
                     /locus_tag="PLP50_15980"
     CDS             5152..5742
                     /locus_tag="PLP50_15980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53093.1"
                     /translation="MRDEQLRRLTLSLLRPEPAERVLDIGCGTGSLAVRLKQEHPRAT
                     VVGYDIDPAALAIAEHKAREAQISVTWARGPADDPPFPDQSFDLVTSSLLLHHLLPSG
                     KRSALAAASRLLKPGGRLVLVDFTRPEGRLRALAFSIVRTLDGRRETEDHAGGRMPAF
                     VEEAGFANVAERLRLNTALGTIGFITAYKPRSGWAP"
     gene            5730..>5947
                     /locus_tag="PLP50_15985"
     CDS             5730..>5947
                     /locus_tag="PLP50_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53094.1"
                     /translation="MGSVKSLRTRIRWWVVASTTLTVGLVTTLGVVEDRRQIERAEVA
                     YLAGFLEHLSAMPEFRADSGTARAHLRML"
BASE COUNT          925 a         2018 c         1952 g         1052 t
ORIGIN      
        1 gccgggctac tgcccgccca tcatcccgcc gcccatgccg cggccgccgt tcatcatgct
       61 gcccatgacg acatagccgt cattcgtcgt gttgtcgttc acgacggcgt aggccatgac
      121 catccccggc ccgccctggt tcatccgcac gcggacctgg aggccggcgt caccggtcgg
      181 gaacttcgaa tggacgttca gaaggacgtc gttgaactgg tgcgaagagt accccgcgag
      241 ggtgaatgga aggctcttgg cgcccgtgcc cgtcggcgtc gacccgtcgg cgtcgacgat
      301 ctcgacgacg ccggtcgcgc ccgtcgacgt cgtgctgacg acattgacgt tcacgcggtt
      361 accgggctgc ggaaggagcc cgagcatgta catcgcgtat tcgtccatgc cgtaagagcc
      421 gcccatgggc atcgaggtca ttcccatccg gctcggcatt cccatgaacg ccaggccata
      481 ggcacccgcc ccggtctggt tgtagacgcg cacggtcgcc gagatcggga tgctcgccgc
      541 tacgcggatc gccccgaagc ccgcagcaag accgaagttg gcctgaagga cgtcgggcag
      601 gtacgtaatc ggctgggtga ggttcaccgt cgccgtggcg gctggggcgg tcgacgtcga
      661 gtccgccgcg tggaagtaga gcgttgccgt tccggtcccg gtcgccttga tccagagatc
      721 cgtgacgaag aaggagctga accggccctc tccacgaaca cccgcaggaa tgaccagatc
      781 ctctgacccg ttcgtcatgc cgtcgatcgg gccaccctgc ccgccgccgc ccatctgggc
      841 cgtggcggga aacgtgacga gggcactgac gagagccgct gcaccgagat aggacttgag
      901 acaccgcata gagacctcct tgtttcggac cgttgacggc cttggctcgt cacgggtcgt
      961 tctatcacta tatggtcata tcgtgtttcg ccctggcgtc gcgccagctg aaacgctagg
     1021 ggctgaacca ccaggggatc gtcaggaagg caagcgtgag ggcgacgacg atccagagca
     1081 gacgccgcca tcgccggtcg cgcgggtctg gattccatgg cctcgaatcg gtcccggtcg
     1141 cgcagcaagc cgcggaactc gtcctgtagg taagcccgac tgacgcccag agcaggacga
     1201 tcgtgccgat ggcgaggtac ggccggtact cgtggaagag ggcgaaggag ccggccccgg
     1261 cgaggccgag catgcctaca gcgggcggac cgatacagca gagcgaggcg ccgattgccg
     1321 agacgagcgc cgcgacgagg ctcattctgc tcagagcacg ggccgctgga ggggtcacga
     1381 tgacgttccg ctatcacccg gacgagccga tccggaccga agcgctgcga cgatcacggc
     1441 tacggaaggc gctccgtggt cacccgcgga accgtaaagg cgacaggacg cgctcccgcc
     1501 tggtgacgcg gatgccccgg agacgtcgac gccgttcacc agaatcgtag gagaccccca
     1561 gacccgggcg cggtcgggaa cgttcggtcc gctagcgtcg agctcgtgcc agacggaagg
     1621 gtggccggct tccgcaaggg ccgcccggag gttcgtgcga gccctgtcgc ggttcgggca
     1681 gtcgtcgaag gcgacgagct cgacaatggg catcggccct acatcccttg cccgagccgg
     1741 acgacatcac gaggctgcac ttcgcgtttc aatgcgcgct ccgtttttgc agggacgcgt
     1801 cggacgaacc ggagccggaa agaaacgcct cgatctgcct tgccttctcg tactcggccg
     1861 ggctcttcgg gttgagggac tccatctccc ggatctcggc cggcgtgagc ttcggaaggt
     1921 ggcggatgaa gtggacgagc tcccacgtgc ctacatcttc cggcggcgtg ccattgcccc
     1981 acgcgggcat gccggtaaaa cggattccat tcctgatgat ggagaagagc tcaccgtcgg
     2041 tgagccgttg ggtctcgccg gaacgcatgt cgggagcccg cggatacagg cggcggccga
     2101 ctggcgtctg cccactcccg tcgtttccgt ggcagctcgc acaatggtcg gcgaagtgtg
     2161 cccgcgcccg ggcgaggacc tcggggccta tggggacggg gttcggcaca cggcgcgccg
     2221 cctccggtgt cgcccacccc cggaaggtcc gtgccacgag ctcctcggcc ttcgtcggct
     2281 cctcgcgagc gctgaaaccc cggcggacgg cggctgcgag ggcgaaaccg cagccgacgg
     2341 cgacgacggc tcccgctacc agtagcactt tgcctgtgcg cgtcacgtcg aatccccttc
     2401 ggcccgactg gccctcaggc cttcttcttc tccaggaaca tgccgcactt cgggcacttc
     2461 ccggggccgg cgttcttcac ctcgggatgc atcgggcagg tccagacagc attctcctcg
     2521 gcgccgatcc ggacgaccct ggcctcgagc cccgacttgc cctcggccgt ctccacggcg
     2581 acgcgcacac cgggcttcag gtcggtcttc ttcgcctccg agtctcctct gcggtacgac
     2641 gtcgcgctcg tgagcgtgac ggagaccgtc ttgccgtcgg ggtccgccac ctcgaggtgg
     2701 tcaccgtgga cctgcgcgac agtaccgagg atcgcttcct tcccatgggc gtgcccgtcg
     2761 tgggcggagg ccacgcccgt cgaaagcgtg agagcgagag ccgcgagaac ggagagagcc
     2821 ttacgcatgt cgatgtctcc tgtcgatcgg ccgctgagcg agcgtctcgc accgcagccg
     2881 tgtatgggcg gaaacatacg acccgtacct tagagcagca ttagagggtg agaggctttc
     2941 tctctcatcg aattctaagg tctggtcccc agtattgagg catggcagga acctctcccc
     3001 gcggcacgct cctcctggtc gaagacgacg cttcgttcgc gttctgcgcc gctgagttcc
     3061 tctcgcggcg cggtttcgag gtagcacacg ctcgcgacct cgactccgcc ctggtccgcc
     3121 tggccgccca gcgctgggcg gccgtcgtca cggacctcga cctcacgggg cggcagacgc
     3181 gagacggcct ggaagtcgtc gccacggcgg cgcgcttcga gccgcgtccc gtgatcttcg
     3241 tctggacgag ctcgtcggcg accgaggtac ggcaggaagc gttccgtctc ggcgccgacc
     3301 tcgtcgtcga gaaagggagc ctctgggacc tggaggcccg gctcagggta tgcctcgcgg
     3361 aacgcccctc acgagtcgcg tcctggccgg aggacgtgga gtccgtccct ccgcccgggc
     3421 tcaccgcagg gtccgagaag acgcccgagt ccgcgtcggc cgggggaagg cggtgaacgc
     3481 agcggagacc ttcgccccgc gctgcgattc cacgccctca cgggtctctt cgacccgttc
     3541 atgtcggccc tcatgaaaga ggcgacgttg cgccggagag gcgggcgccg ggtgctcgcg
     3601 ctccgtctgc ccgggcgggc ttccccctct gcgatcttcc gtgacagcta ctttccccgc
     3661 agtgtttccc actgcttccg atggaaggtg gagaaccgct ccgcctcgag cgcgacggcc
     3721 tccaccttct ctatatacgc ttcgtagtgt cgcctcatct ccagaatcat cggatcggtg
     3781 tccgcccgct ccggttctct gttcccggtg atggaatccc cgagcagctt tcgctgtttg
     3841 gcggcttcct tgcggtacga cgccgccttc ctttcgtatt cctcggctct tgcgagatga
     3901 acctccggga gcctcgcgat ttcgtcggcg aacatggggt aggcccctcc ctcctgttgc
     3961 gcgtccgcga cgagcggcag aagagtgagt ggcgctgcga ggagggcggc aaaggcgagg
     4021 cgctggacct tgtgtgcagc gtggttgagc atggcagctc ctttccatgg gcgggatgac
     4081 ggcaggcgaa gcggagtcgg cacctcgggc ccagacgcgg cgcacccgca cttggacagg
     4141 ctcactggcc gttcagctcg gccgcacgca tttcgtggta ctcggccagc ttctccgcct
     4201 tcgccgccag tgcctctgcg tccttgatgt agccgttgca gtggccccgc agcttctcga
     4261 gctcgggagg ctcgtagttc ccgggccggg tatcgggcgg cagtcgcttc ttcagggcca
     4321 ggatcatctc gcgatgcctg ttcgcctctt cgcggtaagc ggcggccttc tttcgatact
     4381 cgacggccct gtcgaggtgg tccttcgggg tgaccgcgac cttcatcgcg gttcggctct
     4441 cggccgcagc ggcggcctgc acttgtggcc cagcggcagc cggtcgcacg gccgagatca
     4501 gtgcggaaac gacgagaatg actgatgcca gatgccgagt tacccgggga cgctctgcgg
     4561 acatcgcgaa cctcctgcca tcgtcttggg ctgtgcttcg ccggctggcc tacgtttcgg
     4621 gacgagcgtg aaaggacttt gatccggggc attcggaatg ctcccgtttg cctcttgacc
     4681 tcaagaatgg gtcgagtgga ttagaggcgc cttaaaacgc gtgccgctcc ttccacggaa
     4741 aggcatacgg tcgtcgggta ccttcggtcg gatctgagcc agcaagcccc tcggccctgg
     4801 gtcggaacgc gggcggcccg aaacgcgaat cggctaaccg aacggcggac gctgcttccc
     4861 ttcgtctaag gcgcctctaa tctgccgggg ctatcttctc gatcagagga cggcgaaggc
     4921 ggagcagggt ctcaccgaac tcgagttcat tgctccgcca agggtatcgg cggaggaatc
     4981 cgagaggtag gtatgccaaa gagcgaacag ggcctcacgt gcggtcgacc ggtcagcgtc
     5041 gacgccgccg tccgggttct tcagcgcgac cctacgagcg gggccgcata tatcccggct
     5101 ctacgtttcc acgccctgac aggtctcttc gaccctctca tgcagctcgt catgagggat
     5161 gaacagctga ggcgactgac cttgtccctc cttcgccctg agccggcaga gcgcgtgctc
     5221 gacatcggat gcgggaccgg ctctctggcg gtccggctga aacaggagca cccccgcgcg
     5281 accgtcgtcg gctacgacat cgacccggcg gccctcgcga tcgccgagca caaggcacgg
     5341 gaggcgcaga tctccgtgac ctgggcccgc ggcccggccg atgatccgcc cttccccgat
     5401 cagtctttcg acctcgtgac ctcctccctc ctcctccacc atctcctccc ctccgggaag
     5461 cgatccgctc tcgcggcggc gtcgaggctc ctcaagccgg gggggcgcct cgttctcgtc
     5521 gacttcaccc gtccggaagg gcgccttcgt gcgctcgcct tttcgatcgt ccgcacactc
     5581 gacggccgcc gggagaccga ggaccacgcc gggggccgaa tgccggcctt cgtcgaggaa
     5641 gcgggtttcg cgaacgtggc cgagcggctg cggctgaaca cggcccttgg caccatcggg
     5701 ttcatcaccg cgtacaagcc gcgctccggg tgggctccgt gaaaagcctc cggacccgta
     5761 tccgatggtg ggtcgtcgcg tcgaccaccc tcacggtcgg gctcgtcacc acgcttggtg
     5821 tcgtcgagga tcgccggcag atcgaacggg cggaagtcgc ttacctcgct gggttcctcg
     5881 agcacctctc cgcgatgccc gagttccgag cggactcggg gacggcccgg gcgcacctcc
     5941 ggatgct
//
LOCUS       DAOONL010000177         5946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15975_length_5946_cov_17.952130, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000177 DAOONL010000000
VERSION     DAOONL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15975_length_5946_cov_17.952130"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..861
                     /locus_tag="PLP50_15990"
     CDS             121..861
                     /locus_tag="PLP50_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA53095.1"
                     /translation="MRHGKRVAVVMPAYNAEKTLERTFEEVPKEWVDDIFLVDDASRD
                     GTVALARTIPGLTVVVHSENRGYGGNQKTCYATALSHGADVVVMVHPDHQYDASVLHD
                     LIDPIADGSVDAMFGSRMLGGRPIEGGMPKWKYLANVFLTAIENATFYLYLTEYHSGL
                     RAYSRRYLEAVNLASFSDDFVFDTEIIAAGIWKGMRIREIPIVTRYFDEASQISFVRS
                     CVYGFSILGVMVRYKLHKWGWRNDPIFK"
     gene            858..>1717
                     /locus_tag="PLP50_15995"
     CDS             858..>1717
                     /locus_tag="PLP50_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53096.1"
                     /translation="MRRLLPAAVAAIVLLAVGLANLDLLSGRATPSFRDLATTQRPAR
                     ALAASLGDAALNPHASFGQTWRGNPNLVLAYPFPVAPRWLGVHLLLHAALGGFGFLLF
                     LRLLGRSSEAALAGALAFSFSGFALSCTAFLNSATTLAWVPWLLASVAAARLDGRRAL
                     RLALLGSAASGALLAHAGEPALGLVGILLALAVALFGPGGTRLRALGALLGGGALAFL
                     ASAPYLFDVLRATEASARRLRGFSWTEFSAVGFHPLRLLETPFPFLFGDPSRVLSGAY
                     WGFAASQGNPP"
     assembly_gap    1718..1817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1818..2948
                     /locus_tag="PLP50_16000"
     CDS             <1818..2948
                     /locus_tag="PLP50_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53097.1"
                     /translation="LPRWRSRAGLVLLVLAGLLGLAAVAARLGPALPRGLLLSLWDPA
                     WASDPGVVLAPVLARLPQQAASSAALLLVAGLLLARGLGDLRSRALLLVALSAEGLAQ
                     ARFHLPRAPAGLYDRPAPLVAAAARIPGRVFERTWKDVDAVRRGLSGPLPTDERLALA
                     LAQTTQGWSLAGAPHGLRYAWDADPDGSYTLLDRYARDVVLSRAWPERLEWLRAAGVG
                     AVIASDVPPGLAGLVPVYVEGRFGPPATLFRLAEPLPGVRRLSRVRGAASVDAAVAIV
                     DAPEFDPATDGVVAGRPPEGTDLVEPDPGASARVVAEGPDALVVETDGTRPGLLLLDR
                     SFSPRVRATVDGRETTVHAVQINLVGVAVPPGPSRVEVRLAP"
     gene            complement(3167..4135)
                     /locus_tag="PLP50_16005"
     CDS             complement(3167..4135)
                     /locus_tag="PLP50_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPA53098.1"
                     /translation="MSPAVRFPRCSVPAPRLALKVDVDTLRGTVEGVPRLLDLLARHG
                     ARATFLFSLGPDSTGKAAKRVFRRGFVRKVMSASPGVSYGLATMLYGTLLPAPDIGRR
                     KEAVARMREADAAGHLCGIHGWDHVDWHDRLPTMEREEVFSTFRRAAGRFAELFGWPA
                     AVAGAPGWTASGLSVEAYEANGIRVASDTRGGGPFRPLRASGAPAELVEVPSTLPTLD
                     ELLAWEEFRGAAREGTVEHLRRAVASVPGVAVHSIHTEIEGGRAFAGLFARQLAAWAG
                     DGVTFVTLSEAAAAAGAPEALPARPLRRVSVPGRALPVTTGLPPAR"
     gene            complement(4104..5135)
                     /locus_tag="PLP50_16010"
     CDS             complement(4104..5135)
                     /locus_tag="PLP50_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HPA53099.1"
                     /translation="MRVLILGVNGFIGNALTRRILTTTDWKVFGLDVGSDRIEEFLGE
                     KRFRFLEGDIAINREWIEYHVKKCDVVLPLVAIATPATYVKNPLSVFELDFEENLRIV
                     KMAARYGKRVVFPSTSEVYGMCPDAEFDEDASPLVYGPIRKERWIYSASKQLLDRVIW
                     AMGNANGLRFTLFRPFNWYGPHLDDVDAPKEGSSRVLTQFLHNVLYGVPIKLVDGGRQ
                     RRCFTYIEDGIDGLMKILVNENGCADGQIFNLGNPSADLSIRELAERLVAAVGTYEKF
                     APLARAATLVEVSATEYYGANYQDITTRVPSVRKAKECLGWTPTTDFATGLAKTLDFY
                     LSGRPLPEV"
     gene            complement(5155..>5946)
                     /locus_tag="PLP50_16015"
     CDS             complement(5155..>5946)
                     /locus_tag="PLP50_16015"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HPA53100.1"
                     /translation="WFESVAELARSRGVPVKLVESIDAAADEAALRAAAPDFLFSFYF
                     RSMIPARVLSIPSKGALNMHGSLLPAFRGRAPVNWAILKGAPFTGASLHYMTEKPDAG
                     DLVDQEKVPIGPDDDALAVSHRVSEAAVTVLARSWPKLKAGTAPRVPLDLTKGSYFGA
                     RRPEDGLIDWFRPAQEVHDLVRAVSRPWPGAFTDVFGPRVTIWKTRLSGYGGHDTFPG
                     KIDMTERSVLVYCGDDRPVEILAARPEGQPDMNEAELRAWVLARG"
BASE COUNT          746 a         2222 c         2017 g          861 t
ORIGIN      
        1 ctcgttcggc gagacgaagt gggggaagac cggcacgacg aggaacgcga ggagggcgaa
       61 gagccccgtc accttccacg cccccggccc cggcgccgca gtcgtcataa tcttcggatc
      121 atgcgccatg ggaagcgggt cgcggtcgtc atgccggcct acaacgcgga gaagaccctc
      181 gagcggacct tcgaggaggt cccgaaggag tgggtcgacg acatcttcct cgtcgacgac
      241 gcctcccggg acgggaccgt cgccctcgcg cggacgatcc ccggcctgac cgtcgtcgtc
      301 cactccgaga accggggcta cggcgggaac cagaagacct gctacgcgac cgcgctctcg
      361 cacggggccg acgtcgtcgt catggtccac cccgaccacc agtacgacgc ctcggtgctc
      421 cacgacctca tcgacccgat cgcggacgga tccgtcgacg cgatgttcgg gagccggatg
      481 ctcggaggcc gcccgatcga ggggggcatg ccgaagtgga agtacctcgc gaacgtcttc
      541 ctgacggcga tcgagaacgc cacgttctac ctctacctga cggagtacca ctcgggcctt
      601 cgcgcctact cgcggcggta cctcgaggcg gtgaacctcg cctccttctc cgacgacttc
      661 gtcttcgaca ccgagatcat cgcggcgggg atctggaagg ggatgcggat ccgggagatc
      721 ccgatcgtga cccgctactt cgacgaggcc tcgcagatca gcttcgtgag gtcgtgcgtc
      781 tacggcttct cgatcctcgg cgtgatggtc cgctacaagc tccacaagtg gggctggcgg
      841 aacgacccga tcttcaagtg aggcgactcc tgccggcggc cgtcgccgcg atcgtcctcc
      901 tcgccgtcgg gctcgcgaac ctcgacctcc tttccggccg cgcgacgccc tcgttccggg
      961 acctcgcgac gacgcagcgc cccgcccgcg cgctcgccgc ctcgctcggc gacgcggccc
     1021 tgaacccgca tgcctcgttc ggccagacct ggcgcggcaa cccgaacctc gtcctcgcct
     1081 acccgttccc cgtcgccccc cggtggctcg gcgtccacct cctgctccac gcggcgctcg
     1141 gcgggttcgg cttcctcctc ttcctccgtc tcctcggccg ctcatccgag gcggcgctcg
     1201 cgggggcgct cgccttctcc ttctccggct tcgccctctc gtgcaccgcg ttcctgaact
     1261 cggcgacgac gctcgcctgg gtcccgtggc tcctcgcctc ggtcgccgcg gcccggctcg
     1321 acgggcgtcg cgcgctccga ctcgcgctcc tcggctcggc cgcctcgggg gccctcctcg
     1381 cgcacgccgg tgagcccgcc ctcgggctgg tcgggatcct cctcgccctc gccgtcgctc
     1441 tcttcggacc cggcgggacg cgcctgcgcg cgctcggcgc cctcctcggc ggaggcgcac
     1501 tcgccttcct cgcctcggcc ccctacctct tcgacgtcct ccgcgcgacg gaggcctccg
     1561 cccgacgcct ccgcggcttc tcgtggacgg agttctcggc ggtcggcttc cacccgctcc
     1621 ggctcctcga gaccccgttc ccgttcctct tcggcgaccc gtcgcgggtc ctctcgggcg
     1681 cctactgggg cttcgccgct tcgcagggga acccgccnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnctc ccccgctggc gctcccgcgc cggcctcgtc ctcctcgtcc
     1861 tcgccggcct gctcggcctc gccgcggtcg ccgcgcgcct gggcccggcg ctcccgcgcg
     1921 ggctcctcct ctccctctgg gaccccgcgt gggcctccga cccgggcgtc gtcctcgccc
     1981 cggtcctcgc gcgcctcccg cagcaggcgg cctcctccgc ggccctcctc ctcgtcgcgg
     2041 ggctcctcct cgcgcgcggg ctcggagacc tcaggagccg cgcgcttctc ctcgtcgccc
     2101 tgtccgccga ggggctcgcg caggcccgct tccacctccc ccgcgctccg gcggggctct
     2161 acgatcgccc cgctcccctc gtcgccgcgg cggcgcggat tcccggccgc gtcttcgagc
     2221 ggacgtggaa ggacgtcgac gcggtgaggc gcggcctgtc aggtccgctc cccaccgacg
     2281 agcggctcgc tctcgcgctc gcgcagacga cgcagggttg gtcgctcgct ggcgcgccgc
     2341 acggcctccg ctacgcctgg gacgccgatc ccgacgggag ctacaccctc ctcgaccgct
     2401 atgcccggga cgtcgtcctc tcccgcgcct ggcccgaacg gctcgagtgg cttcgcgcgg
     2461 cgggcgtcgg cgccgtcatc gcgagcgacg tccctcccgg cctcgccggc ctcgtccccg
     2521 tctacgtcga gggccgcttc ggcccgcccg cgacgctctt caggctcgcg gagccgctgc
     2581 cgggcgttcg acgcctctcg cgggtccggg gcgccgcctc cgtggacgcc gcggtcgcca
     2641 tcgtcgacgc gccggagttc gacccggcca ccgacggagt cgtcgccggg cgccctcccg
     2701 agggaaccga tctcgtggag cccgacccag gcgcctccgc gcgcgtcgtc gccgagggac
     2761 cggacgctct cgtcgtcgag acggacggga cgcgccccgg actcctcctc ctcgaccgtt
     2821 ccttctcccc gcgggtccgg gcgaccgtgg acggccgcga gacgaccgtc cacgccgtcc
     2881 agatcaacct cgtcggcgtc gccgtccctc ccggcccctc ccgcgtcgag gtccgcctcg
     2941 ccccctgagc gctccgggag cgccgcccgc ccctcccacc ggggatgctg ttccgcgacc
     3001 gctggtcccc gagggccggt gccaggcgtg aaatcgtgta ttgtttccaa caatacacga
     3061 tttcacgcct ggcaccgaag gggcgtggca ccgaagggga ggtcccgaag ggcgagacgg
     3121 acggggaagc gcccgcgaag gagggcgcgg cgagcgagcg gccggctcag cgggcgggcg
     3181 gcagcccggt ggtgaccggg agcgcccgcc ccggcaccga gacccgccgc agtggacgcg
     3241 cgggaagcgc ttcgggggcg ccggcggcgg ccgccgcctc cgagagcgtg acgaaggtga
     3301 cgccgtcgcc cgcccaggcc gcgagctgcc gcgcgaagag gccggcgaat gcgcgccccc
     3361 cctcgatctc cgtgtggatc gagtggaccg cgacacccgg gaccgaggcg accgcccgcc
     3421 ggagatgctc caccgtcccc tcccgggccg ctccgcggaa ctcctcccag gccaggagct
     3481 cgtcgagcgt cggaagcgtc gaggggacct cgacgagctc cgcgggggcg ccgctcgcgc
     3541 ggagcggccg gaacggcccg ccaccgcgcg tgtcggaggc gacgcggatg ccgttcgctt
     3601 cgtaggcctc gaccgagagc ccgctcgccg tccaccccgg cgctcccgcg acggccgccg
     3661 gccagccgaa gagctcggcg aagcgcccgg cggcgcgccg gaacgtggag aagacctcct
     3721 cgcgctccat cgtcggcagc cggtcgtgcc agtcgacgtg gtcccagccg tggatgccgc
     3781 agaggtgccc ggccgcgtcg gcctcccgca ttcgcgcgac cgcctccttc cgccgaccga
     3841 tgtcgggtgc ggggagaagc gtcccgtaga gcatcgtggc gaggccgtac gagacgcccg
     3901 gcgaggcgct catcaccttc ctcacgaagc cacggcggaa gacgcgcttc gccgccttcc
     3961 cggtcgagtc gggcccgagc gagaagagga acgtcgcccg ggcgccgtgc cgcgcgagga
     4021 ggtcgaggag gcgcgggacc ccctcgaccg tcccgcgcag cgtgtcgacg tcgaccttca
     4081 gggcgaggcg cggtgcgggg acgctacacc tcgggaagcg gacggccgga gagatagaag
     4141 tcgagcgtct tcgcgaggcc ggtcgcgaag tcggtcgtcg gcgtccaccc gaggcactcc
     4201 ttcgccttcc gcaccgaggg gacgcgcgtc gtgatgtcct ggtagttcgc gccgtagtac
     4261 tccgtcgccg agacctcgac gagcgtcgcg gcccgggcga gcggcgcgaa cttctcgtag
     4321 gtccccacgg ccgcgacgag gcgctcggcg agctcacgga tcgagaggtc ggcggagggg
     4381 ttgccgaggt tgaagatctg gccgtcggcg cagccgttct cgttgacgag gatcttcatg
     4441 agcccgtcga tcccgtcctc gatgtacgtg aagcagcggc gctgccgccc cccgtcgacg
     4501 agcttgatcg ggacgccgta gaggacgttg tggaggaact gcgtcaggac ccggctcgag
     4561 ccctccttcg gggcgtcgac gtcgtcgagg tgcggcccgt accagttgaa cggccggaag
     4621 agcgtgaagc gcaggccgtt cgcgtttccc atcgcccaga tgacccggtc gaggagctgc
     4681 ttggaggcgg agtagatcca gcgctccttg cgaatcgggc cgtagacgag gggcgaggcg
     4741 tcctcgtcga actcggcgtc cggacacatc ccgtagacct ccgaggtcga gggaaagacg
     4801 acgcgcttcc cgtagcgcgc ggccatcttg acgatccgga ggttctcctc gaagtcgagc
     4861 tcgaagaccg agagcgggtt cttcacgtag gtcgcgggcg tcgcgatcgc gacgagcggc
     4921 aggacgacgt cgcacttctt gacgtggtac tcgatccact cgcggttgat cgcgatgtcc
     4981 ccttcgagga agcggaagcg cttctcgccg aggaactcct cgatccggtc gctgccgacg
     5041 tcgaggccga agaccttcca gtcggtcgtc gtgaggatcc gccgtgtcag ggcgtttccg
     5101 atgaagccgt tgactccgag gatgaggacg cgcatcgcgc ctttccggcc gctctcagcc
     5161 ccgggccagg acccaggcgc ggagctcggc ctcgttcatg tcgggctgcc cctcggggcg
     5221 cgccgcgagg atctccacgg ggcggtcgtc gccgcagtag acgaggacgg agcgctccgt
     5281 catgtcgatc ttcccgggga aggtgtcgtg cccgccgtag ccggagaggc gggtcttcca
     5341 gatcgtcacg cgcgggccga agacgtcggt gaacgccccg ggccagggac gagagacggc
     5401 ccggacgagg tcgtggacct cctgggccgg ccggaaccag tcgatcagtc cgtcctcggg
     5461 tcgcctcgcg ccgaagtagg agcccttcgt caggtcgagc gggacccgtg gggctgttcc
     5521 ggccttcagc ttcggccagc tccgggccag gaccgtcacg gccgcctccg agacgcgatg
     5581 cgagaccgcg agggcgtcgt cgtccggtcc gatcggcacc ttctcctggt ccacgaggtc
     5641 gcccgcatcg ggcttctcgg tcatgtaatg gagagaggcg cccgtgaacg gggccccctt
     5701 cagaatggcc cagttcaccg gagcccgccc ccggaacgcc gggaggaggg agccgtgcat
     5761 gttgagcgcc cccttcgacg ggatcgagag gacgcgggcg gggatcatcg accggaagta
     5821 gaacgagaag aggaagtcgg gggcggcggc gcgcagggcg gcttcgtccg cggccgcgtc
     5881 gatcgactcg acgagcttca ccggcacgcc ccgggagcgg gcgagctcgg cgaccgactc
     5941 gaacca
//
LOCUS       DAOONL010000178         5943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_15988_length_5943_cov_19.295686, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000178 DAOONL010000000
VERSION     DAOONL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15988_length_5943_cov_19.295686"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2543)
                     /locus_tag="PLP50_16020"
     CDS             complement(<1..2543)
                     /locus_tag="PLP50_16020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA53101.1"
                     /translation="MLVLHGNPLPPTGPLAGAGFFLWGEGPFVGARQRPEHPRHVDPS
                     LLVTALRSAGLAVATGASRPLRLVLTVPTLRSRPVPTRSSMREDGDYAAGETVLRPLE
                     VEGLHVPAERAFEALLSLAEEPERSGVVLGDDVEYWSAVAGWVLDLLHRRRFVPAVED
                     GLARWRPVLTDAHEKERAEVFAQAMPAASRAIAWPGSVTEGLYPAAGFLLRGVVDDLT
                     DAAARALLTETMPESRRRGLPGPEGHALARLAVRRDGREDESAAEAIPADVAERFGAW
                     SVPLLEALPEGELRLGVRLLSPEPEAEGAPSWRLGYHLEAADDPSLKLYAEEIWNETA
                     SSLRRLGRRFVNPQETLLARLGGVAKLCDPIRRSLEERHPVGAPLSLEEAWRFLASDA
                     PLLAEAGVQLLLPAEGRTTKPSLRLNAAESRWKSGVAVTRFGLGTLVDFDWKVAVGDL
                     LLSPEEFEQLASRKVPLVEVRGEWVLLDPQSVQRTLALFEKKPSGRATLADFLRMASG
                     LDEDDDSAPGVDVLTADGWLADFLQADAETEAPFVTPKGLAGRLRPYQEKGVTWLRFL
                     LSRGLGSCLADDMGLGKTIQLLATLLTAREAGEAIRPSLLVCPTSVVENWVQEAHRFA
                     PSLTVAVHHGPERAAGDDFFDLVKKTDLLVTTYSLAHRDRALLTEVTWEYLALDEAQN
                     IKNPGTAQSRAVRALRSTRRAALTGTPLENRLSELKAILDFLNPGLLGSDESFRRTFS
                     IPIERHHDPVARERLRRLTSPFLLRRTKTDPAIAPDLPDKIETKEWVGLSREQATLYR
                     ATTKALLEGIGKAQGQSRRAKVLLLLLRLKQICNHPALFLGDGSRLDGRS"
     gene            2607..3119
                     /locus_tag="PLP50_16025"
     CDS             2607..3119
                     /locus_tag="PLP50_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53102.1"
                     /translation="MKPYEIFRRLSTAETDALVLSACEDDEIPDKVAGSVLTLQRVPL
                     KRFERMTDEVRRGLVRKTLRDRRAEDLALFVLSAGLVRARSEMIEGFLASLDLPHDGP
                     NLTADEPVAEPARERLAKAVADLAAKHGARDAALFLHAFVEQPDVAWPVLAELLDGDP
                     KLALEDRSAD"
     gene            3212..3703
                     /locus_tag="PLP50_16030"
     CDS             3212..3703
                     /locus_tag="PLP50_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPA53103.1"
                     /translation="MIPEKLKAALDEHVSAEVGAAYLYLAMSADFESKSYKGFARWMR
                     VQFQEEMAHALKFVDYMLSRGATIGWKDAKAPASTFGSPLETFEKTLAHEKDVTARIH
                     KLYHLAAAEGDTATQIFLQWFVTEQVEEEARVSEIVEKIRMVADRPGSVLYLDKEYGK
                     RSA"
     gene            complement(3928..4725)
                     /locus_tag="PLP50_16035"
     CDS             complement(3928..4725)
                     /locus_tag="PLP50_16035"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPA53104.1"
                     /translation="MRRVRFHARLLALYFAQYAKARLAYRADFAAAVLASFLGTAASF
                     SVVLLLFSRFPHLAGWTYPEIVFLYGFSLLPYGLFSVVSSNLWDFADRYLVEGRFDRI
                     LLRPVSPLFQVLFESFRLESLQEVATGAVAIGWAVSRLPDVSPLGLALLPLWAVFGAV
                     IYVSVFGLLTAASFWVEDRVGLAPPVFNLMSFGRYPITIYDLKVKLLLSTVIPFAFAS
                     FYPTALALGRGEFLPYFWAVPIVAVAFALLNVALWRRGIAKYGSTGS"
     gene            complement(4728..5552)
                     /locus_tag="PLP50_16040"
     CDS             complement(4728..5552)
                     /locus_tag="PLP50_16040"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPA53105.1"
                     /translation="MLATALSSARPYLAFARTSFLNILAYRARYFVGILTYFFNATVW
                     YYIWRALFHEKGEGATLGGFTFPQMLTYVATGWALRSFYFNEVDRDIASQVREGRLAM
                     TLIKPVDVQAMTLSQALGESAFRLVLFTVPIGLLLVLVYPVLPPASPAALALYLLSGL
                     LSALLVGAINFCVGLVAIRTTSILGVLRAKYLVLELLSGLLVPVAFFPEPVRKVLVWL
                     PFLHMNDTPGRIWLGLATGADAARSLGLQAAWVVALLALGRLLWRATARRIEVQGG"
     gene            complement(5570..>5943)
                     /locus_tag="PLP50_16045"
     CDS             complement(5570..>5943)
                     /locus_tag="PLP50_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53106.1"
                     /translation="VLWDGSLRGLRDKHLPEARIRFDLPAPPDGDPAELAVGPWRAAR
                     LSPGRYLLAVDKREASAPDVIREVVRSLPVLDLAVSEPDIEEVVARIYRENAALRRPD
                     APPAAEGAAPGPVAHRGVRRG"
BASE COUNT          922 a         1915 c         2382 g          724 t
ORIGIN      
        1 gagcggccgt cgaggcggct gccgtcgccg aggaagagcg ccgggtggtt gcagatctgc
       61 ttgaggcgga ggaggagcag gagcaccttc gcgcgccggc tctgcccctg ggcttttccg
      121 atcccctcga ggagggcttt cgtcgtggcg cggtagagcg tcgcctgctc gcgcgagagg
      181 ccgacccact ccttcgtctc gatcttgtcg ggaaggtcgg gggcgatggc ggggtcggtc
      241 ttcgtcctgc ggaggaggaa cggggacgtc aggcgccgca ggcgctcgcg ggcgacgggg
      301 tcgtggtgcc gctcgatcgg gatggagaag gtgcgccgga acgactcgtc ggagccgagg
      361 aggccggggt tgaggaagtc gaggatcgcc ttcagctcgg agaggcggtt ctcgagcggc
      421 gtgccggtga gggccgcgcg gcgcgtggag cggagcgcgc ggacggcgcg cgactgcgcc
      481 gtgccggggt tcttgatgtt ctgcgcctcg tcgagcgcga ggtactccca cgtgacctcc
      541 gtgaggaggg cgcggtcgcg gtgggcgagg gagtacgtcg tgacgaggag gtcggtcttc
      601 ttcacgaggt cgaagaagtc gtcgccggcg gcccgctccg gtccgtggtg gacggcgacg
      661 gtgaggctcg gggcgaagcg atgggcctcc tggacccagt tctcgacgac ggacgtgggg
      721 cagacgagga gcgagggacg gatcgcctcg cccgcctcgc gggcggtgag gagcgtcgcc
      781 aggagctgga tcgtcttgcc gagccccatg tcgtcggcga gacagctccc gagcccgcgg
      841 gagaggagga accgcagcca ggtgaccccc ttctcctggt aggggcggag gcgcccggcg
      901 aggcccttcg gcgtgacgaa gggggcttcc gtctcggcgt cggcctggag gaagtcggcc
      961 agccatccgt cggccgtcag gacgtcgacg cccggggccg agtcgtcgtc ctcgtcgagg
     1021 ccgctcgcca tccgcaggaa gtcggcgagc gtggcgcgcc cggagggctt cttctcgaag
     1081 agggcgagcg tgcgctggac gctctgcggg tcgaggagga cccactcgcc gcgcacctcg
     1141 acgagcggga ccttgcggct ggcgagctgc tcgaactcct cgggcgagag gaggaggtcg
     1201 ccgacggcga ccttccagtc gaagtcgacg agggtgccga ggccgaaccg cgtgacggcg
     1261 acgccgctct tccagcggct ctccgcggcg ttcaaccgga gcgacggctt cgtcgtccgg
     1321 ccctcggccg ggaggaggag ctggacgccg gcctcggcga ggagcggcgc gtcggaagcg
     1381 aggaagcgcc aggcctcctc gagggagagg ggggccccga ccgggtggcg ctcttcgagg
     1441 ctgcgccgga tcgggtcgca gagcttcgcg acgccgccga ggcgggcgag gagcgtctcc
     1501 tgcgggttca cgaacctgcg gccgaggcgc cggaggctcg aggccgtctc gttccagatc
     1561 tcctcggcgt agagcttcag gctggggtcg tccgccgcct cgaggtgata gccgaggcgc
     1621 cacgaggggg ctccctcggc ttccggttcg ggagagagga ggcggacgcc gaggcggagc
     1681 tcgccctccg gcagcgcctc gaggagcggg acgctccagg cgccgaagcg ctccgcgacg
     1741 tcggccggaa tggcctcggc ggcgctctcg tcctcccgcc cgtcgcggcg gacggcgagc
     1801 cgcgcgagcg cgtgcccctc ggggccggga agtccgcggc ggcgcgactc cggcatcgtc
     1861 tcggtgagga gggccctcgc cgcggcgtcg gtcaggtcgt cgacgacgcc ccgcaggagg
     1921 aacccggcgg ccgggtagag gccctcggtg acggatccgg gccaggcgat cgcgcggctg
     1981 gcggcgggca tcgcctgggc gaagacctcg gcgcgctcct tctcgtgagc gtcggtcagg
     2041 accggccgcc agcgggcgag cccgtcctcg acggccggaa cgaagcgcct gcgatgaagg
     2101 aggtcgagga cccagccggc gacggccgac cagtactcga cgtcgtcgcc gaggacgacg
     2161 ccggagcgtt cgggctcctc ggcgagcgag agcagggcct cgaaggcgcg ttcggccggg
     2221 acgtgaagcc cctcgacctc gaggggacgg aggaccgtct cgcccgccgc gtagtcgccg
     2281 tcctcgcgca tcgaggagcg cgtggggacg gggcgcgagc gaagcgtggg gaccgtgagg
     2341 acgaggcgga gcggccggga ggccccggtg gcgaccgcga ggccggccga ccggagcgcc
     2401 gtgacgagga gagacgggtc gacgtgccgg gggtgttcgg gacgttgccg cgcgccgacg
     2461 aacgggcctt ctccccagag gaagaatccc gccccggcga gtggccctgt cgggggcagc
     2521 gggtttccgt ggagtacgag caagccgccc attctcgcgc atttcgactc gggcggcgcg
     2581 accgggtatc ctccgcccct gcaacgatga agccgtacga gatcttccgc cgcctctcga
     2641 ccgccgaaac cgacgccctc gtcctttccg cctgcgagga cgacgagatt cccgacaagg
     2701 tcgccgggag cgtcctgacg ctccagcgcg tgccgctcaa gcgattcgag cggatgacgg
     2761 acgaggtccg gcgcggcctc gtgcggaaga cgctccgcga caggcgggcc gaggacctcg
     2821 ctctcttcgt cctctcggcc gggctcgtgc gggcccggag cgagatgatc gaagggttcc
     2881 tcgcctcgct cgacctgccg cacgacggcc cgaacctgac ggccgacgag ccggtggcgg
     2941 agcccgcgcg cgagcgcctc gccaaggccg ttgcggacct cgccgcgaag cacggagcgc
     3001 gagacgcggc gctcttcctc catgccttcg tcgagcagcc ggacgtggcg tggccggtcc
     3061 tcgcagagct cctcgacggc gacccgaagc tcgctctgga agaccgctcc gcggactgag
     3121 ccgggcgccc ggcgggacgg cggcgtccgg ccgggcgtcg gctaacatcc tcatgcgccc
     3181 gcgcccgaag gcgcgcggcc ggaggttgac catgatcccc gagaagctga aggccgccct
     3241 cgacgagcac gtctccgcgg aggtcggcgc cgcctacctc tacctcgcca tgtcggccga
     3301 cttcgagtcg aagtcctaca agggtttcgc gcggtggatg cgcgtccagt tccaggagga
     3361 gatggcgcac gcgctgaagt tcgtcgacta catgctctcc cgcggcgcga cgatcggctg
     3421 gaaggacgcg aaggccccgg cgagcacgtt cgggtcgccg ctcgagacgt tcgagaagac
     3481 cctcgcgcac gagaaggacg tcaccgcccg gatccacaag ctctaccacc tcgccgcggc
     3541 cgagggcgac acggcgacgc agatcttcct gcagtggttc gtgaccgagc aggtcgagga
     3601 ggaggcgcgc gtctccgaga tcgtcgagaa gatccggatg gtggcggacc gccccggctc
     3661 ggtcctctac ctcgacaagg agtacggcaa gcgctcggcg tagccgcgcc cccctttccg
     3721 cgaccgttcg ccgggcgccg tcggctcgag ccgacggcgc ccgccgcatt tccggatccc
     3781 gcgagcgcca gcgtcccttc ggcgcccgcg cagagtgtag agggtagacc tgaccccacg
     3841 tctcgcgcag actgtagagt gtagaccaga cccctgggtc cggcgggggg cgcggggtgg
     3901 gggacgcggg gagggagcag cggcagatca gctgccggtg gacccgtact tcgcgatgcc
     3961 cctgcgccag agggcgacgt tgaggagggc gaaggcgacg gcgacgatcg ggacggccca
     4021 gaagtacggg aggaactcgc cgcgcccgag cgcgagggcg gtcgggtaga acgaggcaaa
     4081 ggcgaagggg atgaccgtcg aaaggaggag cttcaccttc aggtcgtaga tcgtgatcgg
     4141 gtagcggccg aacgacatga ggttgaagac gggaggcgcg aggccgaccc ggtcttcgac
     4201 ccagaagctc gcggcggtca ggagcccgaa gaccgagacg tagatgaccg ccccgaagac
     4261 ggcccagagc ggcaggagcg cgaggccgag gggcgagacg tcggggaggc gcgacacggc
     4321 ccacccgatc gccacggcgc cggtggccac ctcctggagc gactcgaggc ggaagctctc
     4381 gaagaggacc tggaagagcg gcgagacggg gcggaggagg atgcggtcga agcgcccctc
     4441 cacgaggtag cggtcggcga aatcccagag gttcgacgag acgacgctga agagcccgta
     4501 ggggaggagc gagaagccgt agaggaagac gatctcgggg tacgtccagc ccgcgaggtg
     4561 cgggaagcgg gagaagagga ggaggacgac ggagaacgac gccgccgtcc cgaggaacga
     4621 ggcgaggacg gccgcggcga agtcggcccg gtaggcgagc cgcgccttcg cgtactgcgc
     4681 gaagtagagc gcgaggaggc gcgcgtggaa gcggacgcgg cgcaccgtca gcccccctgg
     4741 acctcgatcc ggcgcgcggt cgccctccag aggaggcgac cgagggcgag cagcgcgacg
     4801 acccacgcgg cctggaggcc gagggagcgc gcggcgtctg cgccggtcgc gaggccgagc
     4861 cagatgcgcc ccggcgtgtc gttcatgtgg aggaacggga gccagacgag gaccttcctc
     4921 accggctcgg ggaagaaggc gacggggacg aggagccccg agaggagctc gaggacgagg
     4981 tacttggcgc ggaggacgcc gaggatcgac gtcgtccgga tcgcgacgag gccgacgcag
     5041 aagttgatcg ccccgacgag gagcgcggag aggagccccg agaggaggta gagcgcgagc
     5101 gccgcgggcg aggcgggcgg gaggacgggg tagacgagga cgaggaggag gccgatcggg
     5161 acggtgaaga ggacgaggcg gaaggcgctc tcgccgagcg cctgcgagag cgtcatcgcc
     5221 tggacgtcga ccggcttgat gagcgtcatc gcgaggcgcc cctcgcggac ctggctcgcg
     5281 atgtcgcggt cgacctcgtt gaagtagaag ctccggagtg cccatcccgt ggccacgtac
     5341 gtgagcatct gcgggaacgt gaagccgccg agcgtggcgc cctcgccctt ctcgtggaag
     5401 agcgcgcgcc agatgtagta ccagacggtc gcgttgaaga agtaggtgag gatgccgacg
     5461 aagtagcgcg cgcggtacgc gaggatgttc aggaacgagg tgcgcgcgaa ggcgaggtag
     5521 gggcgcgcgg aggagagcgc ggtcgcgagc atcggcgggg ggggcgccct cagccccgcc
     5581 gcacgccgcg gtgggcgacg ggccccgggg cggcgccttc ggccgcgggc ggggcatcgg
     5641 gccgccgcag cgccgcgttc tcgcggtaga tgcgggcgac gacctcctcg atgtcgggct
     5701 cgctgacggc gaggtcgagg acggggaggc tcctcacgac ctcgcggatc acgtcggggg
     5761 ccgaggcctc ccgcttgtcg acggcgagga gatagcggcc cggcgagagg cgggcggcgc
     5821 gccacggtcc gacggcgagc tccgccgggt ccccgtccgg gggagcgggg aggtcgaagc
     5881 ggatccgcgc ctcggggagg tgcttgtcgc gcaggccgcg caggctgccg tcccagagga
     5941 cct
//
LOCUS       DAOONL010000179         5936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_16018_length_5936_cov_17.416766, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000179 DAOONL010000000
VERSION     DAOONL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16018_length_5936_cov_17.416766"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..2743)
                     /locus_tag="PLP50_16050"
     CDS             complement(20..2743)
                     /locus_tag="PLP50_16050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA53107.1"
                     /translation="MARCGGPLEDGTGTMDAGLPAPFEGESPGVRLFAPGTDIAARYE
                     VLSVRGVGGSAVVYAAFDRELRQPVALKVLRADRTSAAALTRFRREAAIARQAASPRL
                     VRVYDIEATGESVFLTMEDVAGGSLKDRLSDGPLPFEDGLRVAGEILEGLAALHGLGI
                     VHRDVKPGNVLLTADGSVKLADFGLARRLQADETRATSMDAVVGTIDYVSPEQALGRE
                     LDGRSDLYSFGVTLYEALTGSVPFRRDSAIGTALAHLKEPPPRLRSVRPDAPAWLDAF
                     VARLLSKAPEERYATAEEALADLAARRATGSPTRPRRRRLVVLSLALAATAAILVGIL
                     LLRPFRRPEAVRLEPLANGRGVVAVDPAGRALWEVPDAPDGGAVRLYRRAGGGNGVAA
                     VLGSGVVGPEGARIALLDAASGTVRKEIRVSAHGVGDFWELPERWVFGGLEVVDMDGR
                     DALVFCIVNPELYPSTTFLVDPENGASRVLLAASGHSVFLGATDLDGDGRKELLFGVS
                     ANRLGQYAAVAAVRPDEPGESADTDTRAAIPPASPDLPVFERQRGRMVWFALGPAGRS
                     SGSRRLEVDEARRVLRLTGSTAEPFELGFDGFAAGSRTALDARSRQEARTSTWRLLLR
                     AARLEKGGDLAGAAAAAKEAVAAIAPAGDRSLLEWARRSEARLRIAGGGIAEGERLFR
                     EIAADAVAPTSVAADAARALHLAGEPTRALRWYRKAVLGHGNAEEAWIVGDALADALL
                     AFGELRRWEEALSLVEATPAMNWSQRAEMRALVFWQAGQPTPEPTGVTPSTSLRRYWL
                     LEERLAAGADPAAILHDLVAEKAGRSDVGELLALLEAELHLRAGRGREAWRAASPAFS
                     SLWARRFRDVTARGHLSLAADRAVRAAAATGESAEAARIRTEARLFLSASR"
     gene            2819..3637
                     /gene="proC"
                     /locus_tag="PLP50_16055"
     CDS             2819..3637
                     /gene="proC"
                     /locus_tag="PLP50_16055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPA53108.1"
                     /translation="MLSGKRIAIIGSGTMGRALAGGLLRSGKVEPGQLAGTARTRQTA
                     EKLSHELGLACTTDNAEAARSADILVLCLKPKDVVKACEGLAARSALVHAPLVVSIAA
                     GVSTGALEAALGSGVPVVRAMPNTPCFIGKGMTVVSKGAHATDAHAATALEIFSTMGR
                     TMELEEKHMDTVTALSASGPAFLYVVLESLADGGVMRGLPRAAAIELAAQAALGAAEM
                     VLQTGRHPAALKDDVTTPAGCTIAGILAMEDGRIRSVLARGIETAARAAGDLGK"
     gene            complement(3674..4546)
                     /locus_tag="PLP50_16060"
     CDS             complement(3674..4546)
                     /locus_tag="PLP50_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPA53109.1"
                     /translation="MRRAGTERREVDTADRSRSGERRRREKGPAAGGAVASGTALPTT
                     PCCDSCDVAKTYAAEKARLAVWLAHELRTPLNCVLGYTELLCDQLEAPGHAPLLADAL
                     RVRDLALHQLTLLDEVLDLARLEHGSLTLRVEAIDATRVLEGIAAAALPIVRRGGNRV
                     LLTAEGPAWVLADETRLRQVLLNLVGNAAKFTRDGLVTLGATEANGSVVLHVSDTGPG
                     MAPGVAERLFRPFQQASAAVAAEHGGSGLGLAISKELCERMGGSISLRTAEGEGTTFT
                     VTLRAAGKPEATAA"
     gene            4718..5749
                     /locus_tag="PLP50_16065"
     CDS             4718..5749
                     /locus_tag="PLP50_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006722366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPA53110.1"
                     /translation="MKPNTLNVNREKPATVHNGWTMLVFALLLLAADVALFIWSIVAG
                     VQAKGDPYVLPFVVSLLGLPAWIVFVTGFFTLQPNEARVLVLFGDYKGTVREAGFHWG
                     NPFYSNGSGAAAAAAAAAASAEGHGAKPAALAAQAAQAAGRRSAKRNRISLRARTLNG
                     QRLKVNDKGGNPIEIAAVVIWRVQDTAMAMFDVDDYEQYVETQSESALRHIASLYSYD
                     HGEEDEITLRTNVEEVSAALKTELHERLAKAGVVVEEARLTHLAYAPEIAQAMLRRQQ
                     AEAVIAARQKIVHGAVSMVEMALKELADKNVLTLDDERKASMVSNLMVVLCGDSEVHP
                     VVNAGTLYA"
     gene            5756..>5936
                     /locus_tag="PLP50_16070"
     CDS             5756..>5936
                     /locus_tag="PLP50_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53111.1"
                     /translation="MPTRAAGPPPPGESRKAFLLRIDPALFAEIERWAADELRSVNGQ
                     IEYLLREAVKARRRGR"
BASE COUNT          830 a         2211 c         2140 g          755 t
ORIGIN      
        1 cggccgacct ttcggcccct caccgggacg cggagaggaa cagccgggcc tcggtccgga
       61 tccgcgcggc ctccgcactc tctcccgtcg cagcggcggc gcggacggcg cggtcggcgg
      121 cgagcgagag gtgcccgcgc gccgtcacgt cgcggaagcg ccgggcccag agcgacgaga
      181 aggccgggga ggcggccctc caggcctcgc gtcccctgcc cgcgcggagg tggagctccg
      241 cctcgaggag ggcgaggagc tccccgacgt cggaccgtcc cgccttctcg gcaaccaggt
      301 cgtggaggat cgccgcgggg tcggcgcccg cagcgagacg ctcttcgagg agccagtagc
      361 ggcgcaggga cgtggacggc gtcacgcccg tcggctccgg cgtcggctgc cccgcctgcc
      421 agaagacgag cgccctcatc tcggccctct ggctccagtt catggccggc gtcgcctcga
      481 cgagagagag cgcctcttcc caccgccgga gctcgccgaa ggcgaggagc gcgtcggcga
      541 gcgcgtcgcc cacgatccac gcctcctccg cgttgccgtg gccgaggacg gctttgcggt
      601 accagcggag ggcgcgcgtc ggctcgccgg cgaggtggag ggcccgggcc gcgtcggcgg
      661 cgacggaggt cggagccacg gcgtcggcgg cgatctcgcg gaagagccgc tcgccttcgg
      721 cgatcccgcc gccggcgatc cggagccggg cttcgctcct tcgcgcccac tcgaggaggg
      781 accggtcgcc cgcgggcgcg atcgcggcga cggcctcctt cgccgccgcc gcggcacctg
      841 ccaggtcgcc ccccttctcg aggcgtgccg cgcgcaggag gaggcgccac gtcgaggtcc
      901 gcgcttcctg gcgagaacgc gcgtcgagcg ccgtccggct cccggcggcg aacccgtcga
      961 acccgagctc gaaaggctcg gcggtcgaac ccgtgagccg gaggacccgt cgagcctcgt
     1021 cgacctcgag gcgcctcgag ccggagctcc gcccggccgg cccgagggcg aaccagacca
     1081 ttcggccccg ttgccgctcg aagaccggga ggtcggggga cgcggggggg atcgccgccc
     1141 gcgtgtcggt gtccgcggac tcccccggct cgtccgggcg gacggcggcg actgcggcgt
     1201 actgcccgag ccggttcgcc gagacgccga agaggagctc tttccgcccg tccccgtcga
     1261 ggtccgtcgc tccgaggaag acgctgtgcc cgctcgccgc gaggaggacc cgcgaggctc
     1321 cgttctcggg atcgacgagg aaggtcgtcg aaggatagag ctcgggattc acgatgcaga
     1381 agacgagggc gtccctcccg tccatgtcga cgacctcgag gccgccgaag acccagcgct
     1441 cggggagctc ccagaagtcc cccacgccgt gcgcggagac gcggatctcc ttccggaccg
     1501 tgcccgaggc cgcgtcgagg agcgcgatcc tcgcgccctc cggtccgacg acgcccgatc
     1561 cgagcaccgc cgccacgccg ttccctccgc cggctctccg gtagagccgg acggctccgc
     1621 catccggggc gtcgggcacc tcccagaggg cacggccggc cgggtcgacc gccacgacac
     1681 cccgaccgtt cgcgagcggc tcgagccgga cggcctcggg ccttcggaac gggcggagaa
     1741 ggaggatgcc gacgaggatc gcagcggtcg ccgcgagcgc gagggagagg acgacgaggc
     1801 ggcgccgccg cggccgcgtc gggcttcccg tcgcgcgccg tgccgcgagg tccgcgagcg
     1861 cctcctccgc cgtcgcgtaa cgctcctccg gcgccttgga gaggagccgc gcgacgaagg
     1921 cgtcgagcca cgccggggcg tcgggccgca ccgagcggag tcgcggcggc ggctccttca
     1981 ggtgcgccag ggccgtcccg atggccgagt cgcgccggaa cgggacgctt cccgtcagcg
     2041 cctcgtagag cgtgacgccg aacgagtaga ggtcgctccg gccgtccagc tctcgcccga
     2101 gcgcctgctc cggcgagacg tagtcgatcg tcccgacgac ggcgtccatc gaggtcgccc
     2161 gggtctcgtc cgcctggaga cgacgcgcga ggccgaagtc cgcgagcttc acggatccgt
     2221 cggccgtgag gaggacgttc cccggcttga cgtcgcggtg gacgattccg aggccgtgga
     2281 gagccgcgag cccctcgagg atctcccccg cgacccgaag cccgtcctcg aacggcaacg
     2341 gcccgtcgga cagccggtcc ttgagggagc cgccggcgac gtcctccatc gtgagaaaga
     2401 ccgactcccc cgtcgcctcg atgtcgtaga cgcggacgag gcgggggctc gcggcctgcc
     2461 gggcgatggc cgcttcgcgc cggaaccgcg tcagggcggc cgcgctcgtc cggtcggccc
     2521 gcaggacctt cagcgccacg ggctgccgca gctcgcggtc gaacgccgcg tagacgacgg
     2581 ccgacccgcc gacgccccgg accgaaagga cctcgtagcg cgccgcgatg tcggtcccgg
     2641 gcgcgaagag gcgcaccccc ggcgactcgc cttcgaacgg cgcggggaga ccggcgtcca
     2701 tcgtcccggt gccgtcttcc agggggcctc cgcagcgagc cattctacgg ctcgacccgg
     2761 ccgatcgccc ccctcgcgta cgatccgccc gtactcagac gggacaggag acggatcgat
     2821 gctcagcgga aagcggatcg ccatcatcgg ctcggggacc atgggacgcg cgctcgcggg
     2881 agggctcctc cggtcgggca aggtggagcc gggccagctc gcagggaccg cccgcacgcg
     2941 ccagaccgcc gagaagctct cccacgagct cggcctcgcc tgcacgaccg acaacgccga
     3001 ggcggcccga agcgcagaca tcctcgtcct gtgcctgaag ccgaaggatg tcgtgaaggc
     3061 gtgcgaaggg ctcgccgcgc ggagcgcgct cgtccacgcg ccgctcgtcg tctcgatcgc
     3121 cgccggggtc tcgacgggtg cgctcgaggc ggccctgggc tccggcgtcc cggtcgtccg
     3181 ggcgatgccg aacaccccct gcttcatcgg gaaggggatg acggtcgtct cgaagggagc
     3241 gcacgccacg gacgcgcacg cggcgactgc actcgagatc ttctcgacga tgggccggac
     3301 gatggagctc gaggagaagc acatggacac ggtgaccgcc ctttcggcga gcggccccgc
     3361 gttcctctac gtcgtcctcg agtcgctcgc cgacggcggg gtgatgcggg ggcttccccg
     3421 ggcggcggcg atcgagctgg ccgcgcaggc ggcgctcggc gccgcggaga tggtcctcca
     3481 gacgggacgc cacccggcgg cgctcaagga cgacgtgacg acgccggccg gctgcacgat
     3541 cgccggaatc ctggcgatgg aggacggacg aatccggtcc gtcctcgccc gcggcatcga
     3601 gacggcggcg cgtgccgcag gcgacctcgg gaagtagacg cgaagggccg tcgcgatccc
     3661 ttatccgccg ggctcaggcc gcggtcgcct ccggcttccc ggccgcgcgg agggtgacgg
     3721 tgaaggtcgt cccctctccc tcggccgtcc gcagcgagat cgacccgccc atcctctcgc
     3781 acagctcctt cgagatcgcc aggcccaggc ccgagccgcc gtgctcggcg gcgacggccg
     3841 cgctcgcctg ctggaacggg cggaacagcc gctccgccac gcccggggcc atccccggac
     3901 cggtgtcgct gacgtggagg acgacggagc cgttcgcctc cgtcgcgccg agcgtgacga
     3961 ggccgtcgcg ggtgaacttc gcggcgttcc cgacgaggtt caggaggacc tgccggaggc
     4021 gcgtctcgtc ggcgaggacc caggccggcc cctcggccgt caggaggacc cggttccctc
     4081 cccgcctcac gatcgggagc gccgcggcgg cgatcccctc gaggacccgt gtcgcgtcga
     4141 tcgcctcgac cctcagcgtc aggctcccgt gctcgagccg ggcgaggtcg agaacctcgt
     4201 cgaggagcgt gagctgatgg agcgcgaggt ccctcacccg cagggcgtcg gcgaggagcg
     4261 gcgcgtgccc cggcgcctcg agctggtcgc agaggagctc ggtgtagccg aggacgcagt
     4321 tcagcggcgt ccggagctcg tgcgcgagcc agacggcgag ccgcgccttc tcggccgcgt
     4381 acgtcttcgc gacgtcgcac gagtcgcagc acggtgtggt cgggagggcc gtcccgctcg
     4441 cgaccgcacc cccagcggcg ggccccttct cccgtctcct ccgctcgccg cttcgagagc
     4501 gatccgccgt atcgacctcc cgtcgctccg ttccggcccg ccgcacgtcc cgtctgcgat
     4561 ctatcgggtc gactatgccg ctcccccctg cactgcggca tcccccgaac gggtgaggcg
     4621 aagcgcctcc ccggtccgac ggagcggaaa cctcccgctt gacacggcgt atttcacggt
     4681 gatatcactt cagtagcagc cgacaggagg tcgcgccatg aaaccgaaca ccctgaacgt
     4741 caaccgggag aagccggcga cggtccacaa cgggtggacg atgctcgtct tcgccctgct
     4801 ccttctcgcc gccgacgtcg ccctcttcat ctggtcgatc gtggcgggtg tgcaggccaa
     4861 gggggacccc tacgtccttc ccttcgtcgt ctcgcttctc ggcctccccg cctggatcgt
     4921 cttcgtaacc gggttcttca cgctccagcc gaacgaggcg cgggtcctcg tcctcttcgg
     4981 cgactacaag gggacggtcc gggaggccgg gttccactgg ggcaacccct tctactcgaa
     5041 cggctcgggc gcggcggccg ccgcggcggc cgccgccgcc tcggccgagg ggcatggcgc
     5101 gaagccggcg gccctcgccg cccaggccgc gcaggccgcg ggccggcgct ccgcgaagcg
     5161 gaaccggatc tccctccgcg cccgcactct gaacggtcag cgactgaagg tgaacgacaa
     5221 ggggggcaac ccgatcgaga tcgccgcggt cgtcatctgg cgcgtccagg acaccgccat
     5281 ggcgatgttc gacgtcgacg actacgagca gtacgtcgag acgcagagcg agtcggccct
     5341 ccggcacatc gcgagcctct acagctacga ccacggcgag gaggacgaga tcacgctccg
     5401 gacgaacgtc gaggaggtct cggccgcgct gaagacggag ctccacgagc ggctcgcgaa
     5461 ggcgggcgtc gtcgtggagg aggcgcgcct cacgcacctc gcctacgccc cggagatcgc
     5521 ccaggcgatg ctccggcgcc agcaggccga ggccgtcatc gccgcgcggc agaagatcgt
     5581 ccacggggcc gtctcgatgg tcgagatggc gctcaaggag cttgccgaca agaacgtcct
     5641 gacgctcgac gacgagcgga aggcctcgat ggtcagcaac ctgatggtcg tcctctgcgg
     5701 cgactccgag gtccacccgg tcgtcaacgc cgggaccctc tatgcctgag gagtgatgcc
     5761 gacccgcgcc gccggaccgc cgcctcccgg ggagtcccga aaggccttcc tcctccggat
     5821 cgaccccgcc ctcttcgccg agatcgagag gtgggcggca gacgagctcc ggagcgtgaa
     5881 cggccagatc gagtacctcc tgcgggaggc ggtgaaggcc cggcggcgcg ggcggg
//
LOCUS       DAOONL010000180         5916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_16087_length_5916_cov_18.470909, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000180 DAOONL010000000
VERSION     DAOONL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16087_length_5916_cov_18.470909"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLP50_16075"
     CDS             <1..892
                     /locus_tag="PLP50_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53112.1"
                     /translation="MNIAKGLGALSLLLSTMVPSANALAQEHGTTQWIVTTAKATGHD
                     GEQYESVLRVLNLSPYDAPLRLTFFPQTSLNASRSAFGDPSAASTVDVVIPAGLKVAF
                     WDALGLFGRTAGAGAIRIVSTGTDPQGNPIPVSASSMTKTYPAGPESYAPVTGPLIAA
                     QGPDTLVGAGEPAAIPFLWLGGDDLNGAYRSNIFLASANTDSQTVVRLTLVTNGNVAQ
                     ESREITLGPLTQTQINNVFFYFDFVSFCPPCPSVDMGPEEVRVDVSVLSGGPVAVGAA
                     VIDELNGTSVYVPAVKRATP"
     gene            1042..1545
                     /gene="tpx"
                     /locus_tag="PLP50_16080"
     CDS             1042..1545
                     /gene="tpx"
                     /locus_tag="PLP50_16080"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPA53113.1"
                     /translation="MERTGVVTFLGNPLTLVGPAIKAGEKAPGFTVLDNGLAPKTLAD
                     FAGKTVVLSVTPSLDTPVCDAQLRRFNEAATKLGDDVVVLNVSMDLPFANKRFCSVAG
                     IEKAMTLSDHRDASFGQAYGVLVKELRLLARAIFVVGKDGLVKHAEVVPEMTHHPDYE
                     KALAAAK"
     gene            complement(1624..3186)
                     /locus_tag="PLP50_16085"
     CDS             complement(1624..3186)
                     /locus_tag="PLP50_16085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPA53114.1"
                     /translation="MLIPTGVVENEVRRTPWVSVSLLCLLVAVFLVGVRGGFGDRQRR
                     HLGEKVEQALEFWAERPWLRPPVALGELVGPGVAAEISAEVSQLRDVAVARKLVPISY
                     VVAEQQRQLESLFAEAAGAARDRRDLGLAFVPARPAIAGLVGHMFLHGDFWHLIGNLL
                     MLFITAPFLEDVYGRVLFGALYLVGGLFAAGAHAMATLSPEVPLVGASGALAVVMGAF
                     LVRLGRSHIRFLFLPILFLPTLRFGFALPAFVVLPLWFVEQVVSAQLAPDAPIAWWAH
                     IGGFAFGLAAALTVKLFRVEERWIDPAIEGKIGWAQDPALLKASDARAAGAFATARNS
                     ARAVLTKDPGNVDAWRSLLDTETASGRKAEAASAATRLLERYVAMQEHALAAALVDEV
                     GEVLLEELPARFFLAAATARERSGDAEGAYRELETLAATRPNDPAAVRAAMRMAVLAR
                     GGGREDDALDLLAWAKAHPACEAGFREAASRTEAEIAAASGRPAGGRRSLPARRFAAW
                     GEPRTLPAHADG"
     gene            complement(3374..3751)
                     /locus_tag="PLP50_16090"
     CDS             complement(3374..3751)
                     /locus_tag="PLP50_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPA53115.1"
                     /translation="MKPRARDGEASEPARLRPRLRVVRGGEVLLGPGMAQLLSALRRT
                     GSLRLAAAEMGMSYMHAWGLVRTMNRGFGEPLVTLERGGAAHGGARLTERGAEVLSLY
                     REMEAAARRAAQGPWRRLRRLLS"
     gene            3847..5916
                     /locus_tag="PLP50_16095"
     CDS             3847..5916
                     /locus_tag="PLP50_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA53116.1"
                     /translation="MKTPLLPLLLAASCAAVVPSAARAEEPPAGWEAYALARVTVTAE
                     AAPVDETALTVAVTAEEIAARNAKNVAEALAAVPGLRVSTGRKNEPTVYLHGFDQSRV
                     QVLIDGVPYYEANYGKLDLAQIPTDNIARIEVTKGAASVLGGANALGGTINIISRTAG
                     TKPFTELLVEGGDYATGRFVATHGARRGPLSYWLNLSGQTSGGTPVPGGFTPRTGTIQ
                     QKNPTKNTPAVIEDGGRRENSDTKQLNAWAKLGWEPSKATALFLNLRYFDRDKGAPPA
                     TDAVQVFLKRPAFSQFSRIPTYRDVAFDLDGRHELAKGLTLKAKAFHHEHVDDYESYK
                     DETYSERIAISRYDDTLTGGSLVAEWAAAPWSTLRLAAHLKDDTHRERDDAYLPFAES
                     ASITGSLGLEDSFTLSSRLSAVAGVSYDWFDVSRAERNVTDGSTGDFVRQDRLATPND
                     DQLNPMAGLVWRSASGTVVNASLARKSRFPTLSQLYSTKSGNTALASETSVNATAGVS
                     HPLGANVRVEAGAFLYDVSDMITRNGSSATNLYLNVGKVRMAGFETLLEVTPLPGLTL
                     RADYTFVDAKERTAVRVTDDVTNVPRHKAGFLASWAATTGTRVDLDAAWLGSMYSSLP
                     SVAYPKDPVKESDGYVLLGGKVTQEVLDGLRLWVSGRNLLDEDYESEVGFPGVGRSFS
                     FGASATF"
BASE COUNT          919 a         2261 c         1973 g          763 t
ORIGIN      
        1 catgaacatc gcgaagggcc tgggcgctct ttccctgctc ctgtccacca tggtcccctc
       61 ggcgaacgcg ctcgcccagg agcacgggac gacccagtgg atcgtcacga cggcgaaagc
      121 gaccgggcac gacggcgagc agtacgagtc cgtcctgcgc gtcctgaacc tcagccccta
      181 cgacgcgccc ctgcgcctca cgttcttccc gcagacgtcg ctgaacgcca gcaggagcgc
      241 cttcggcgac ccttccgccg cctcgaccgt cgacgtcgtg atccccgccg ggctgaaggt
      301 ggccttctgg gacgccctcg ggctcttcgg ccgcacggcc ggtgccggcg ccatccggat
      361 cgtctccacc gggacggacc cgcaggggaa cccgattccg gtttcggcgt cctcgatgac
      421 caagacctac cctgccggcc cggagtccta cgcacccgtg acgggccccc tcatcgcggc
      481 gcagggaccc gacacgctcg tcggagcggg cgagcccgcc gcgattccct tcctctggct
      541 ggggggggac gacctcaacg gcgcgtaccg gagcaacatc ttcctcgctt ccgcgaatac
      601 cgactcccag accgtcgtca ggctgacgct cgtcacgaac ggaaacgtcg cgcaggagag
      661 ccgggagatc acgctcggtc cgctcaccca gacgcagatc aacaacgtct tcttctactt
      721 cgacttcgtc tctttctgtc ctccctgccc ctcggtcgac atgggccccg aggaggtccg
      781 cgtcgacgtc tccgtcctga gcggcggccc tgtcgccgtc ggcgccgccg tcatcgacga
      841 gctgaacggt acgagcgtct acgtccccgc cgtgaagcgg gccacgccct agcggcccgg
      901 ggaagctccg gagacctggc gaccgccggc ccgacgggag gatgagtcgg ccggcggttc
      961 ctcgaccagg caccccgtgc tcggccgggg aggagcgata ctccgagaga gcttcaaccc
     1021 ggacgaaagg gagagacctg catggaaaga accggagtcg tcacgttcct cggcaacccg
     1081 ctcacgctcg tcggcccggc catcaaggcc ggcgagaagg cccccggctt caccgtcctc
     1141 gacaacggcc tcgccccgaa gacgctcgcc gacttcgccg gcaagaccgt cgtcctctcc
     1201 gtcacaccgt cgctcgacac cccggtctgc gacgcgcagc tccgcaggtt caacgaggcg
     1261 gcgacgaagc tcggcgacga cgtcgtcgtc ctgaacgtct cgatggacct tccgttcgcg
     1321 aacaagcgct tctgctctgt cgccggaatc gagaaggcga tgacgctgag cgaccaccgc
     1381 gacgcctcgt tcgggcaggc gtacggcgtc ctcgtcaagg agctccgcct tctcgcccgc
     1441 gcgatcttcg tcgtcggtaa ggacgggctc gtgaagcacg ccgaggtcgt ccccgagatg
     1501 acccatcacc ccgactacga gaaggctctc gccgcagcga agtagcgggc ctccgctccg
     1561 gcgggggacc gggggccgcc gcgacgaggc gtcgcggcgg cctttcgcgc gtcccgacgc
     1621 ccgtcagccg tccgcgtgcg cgggaagcgt ccggggctct ccccaggccg cgaaccgacg
     1681 cgccgggagc gatcgcctgc ctcccgcggg gcggcccgat gcggcggcga tctccgcctc
     1741 cgtgcgcgag gccgcttcgc ggaaccccgc ctcgcaggcg gggtgcgcct tcgcccaggc
     1801 gaggaggtcg agggcgtcgt cctcccttcc gcccccccgg gcgagaaccg ccatccgcat
     1861 cgcggcccgg acggcagcgg ggtcgttcgg ccgtgtcgcc gcgagcgtct cgagctcgcg
     1921 gtacgccccc tcggcgtccc ccgacctctc ccgcgccgtc gcggcggcga ggaagaagcg
     1981 cgccgggagc tcttcgagga gcacctcgcc gacctcgtcg acgagcgcgg cggcgagcgc
     2041 gtgctcctgc atcgcgacgt acctctcgag gaggcgcgtc gcggccgagg cggcctcggc
     2101 cttccgcccc gaggcagtct cggtgtcgag gagcgatcgc caggcgtcga cgttcccggg
     2161 gtccttcgtc aggaccgcgc gcgccgagtt ccgcgccgtc gcgaaggctc cggcggcgcg
     2221 ggcgtcgctc gccttcagga gagccgggtc ctgcgcccag ccgatcttcc cttcgatcgc
     2281 cgggtcgatc caccgttcct cgacgcggaa aagcttcacg gtgagcgcgg cggcgaggcc
     2341 gaaggcgaag ccgccgatgt gcgcccacca ggcgatcgga gcgtcgggcg cgagctgggc
     2401 cgagacgacc tgctcgacga accagagcgg gaggacgacg aaggccggca gcgcgaaccc
     2461 gaaccggagc gtcgggagga agaggatcgg taggaagagg aaccggatgt gggaacggcc
     2521 gagacggacg aggaaagcgc ccatcactac cgcgagcgcg ccggacgcgc cgacgagcgg
     2581 cacctcgggg gagagcgtcg ccatcgcgtg agcgcccgcg gcgaagaggc ccccgacgag
     2641 gtagagcgct ccgaagagga cccgcccata gacgtcctcg aggaacggcg ccgtgatgaa
     2701 gagcatcagg aggttcccga tcaggtgcca gaagtccccg tggaggaaca tgtggccgac
     2761 gagaccggcg atggcgggcc gggccgggac gaacgcgagc ccgaggtcgc gccgatcccg
     2821 cgccgccccc gccgcctcgg cgaagagaga ctcgagctgc cgctgctgct ccgcgacgac
     2881 gtacgagatc gggacgagct tcctcgcgac ggcgacgtcc cgcagctggg agacctccgc
     2941 ggagatctcc gccgcgacac ccggcccgac gagctctcca agcgcgacgg gcggcctgag
     3001 ccacggccgc tcggcccaga actccagggc ctgctcgacc ttctcgccga gatggcggcg
     3061 ctgccggtcg ccgaagccgc cccggacccc gacgaggaag acggcgacga ggaggcagag
     3121 gagagagacc gagacccagg gggtcctccg gacctcgttc tcgacgacac cggtcgggat
     3181 cagcatggcc tgccgcgcac ccgacttggg aggttaccgc atcgagaccc ggggtacgcc
     3241 ggcagattcc tccggtcccg ggtcccgggc gacggccccg tgtccgggct tcgatcgccg
     3301 cggctcgccg atatatcccg taaaataggt cggtttgacg attcgtccct cggggccgcc
     3361 ggcccccgat cgctcaggag agaagccgcc ggaggcgccg ccaggggccc tgcgccgcgc
     3421 gccgagccgc ggcctccatc tcccggtaga gcgacagaac ctccgccccc cgctcggtga
     3481 gacgcgcgcc gccgtgcgcc gcgccgcctc gctccagcgt cacgagcggc tcgccgaacc
     3541 cgcggttcat cgtccggacg agcccccagg cgtgcatgta ggacatcccc atctcggcgg
     3601 ccgccaggcg caggctgccg gtccgccgga gcgcggagag gagctgcgcc atccccggtc
     3661 cgagaaggac ctccccgccg cgcacgacgc gaaggcgcgg ccggagccgc gccggctccg
     3721 acgcctcgcc gtccctcgct ctcggcttca ccctcgtcac gacatctctc cggcgacgtt
     3781 ccagtctctc agcttctccc ggaagccgga aggccggagc gcgcgccgct gccgaacgga
     3841 ggccccgtga agactcccct tctcccgctt ctcctcgcgg cgtcctgcgc cgccgtcgtc
     3901 ccatccgccg cccgggccga ggagcccccg gccgggtggg aggcctacgc cctcgcgcgc
     3961 gtcaccgtca ccgcggaggc ggcgccggtc gacgagaccg ccctcaccgt cgcggtcacg
     4021 gccgaggaga tcgccgcccg caacgcgaag aacgtcgccg aggcgctcgc ggccgtgccc
     4081 ggcctgcgcg tctcgacggg gcggaagaac gagccgacgg tctacctgca cggcttcgac
     4141 cagagccgcg tccaggtcct catcgacggc gtgccgtact acgaggcgaa ctacggcaag
     4201 ctcgacctcg cccagatccc gaccgacaac atcgcgcgga tcgaggtgac gaagggggcc
     4261 gcctccgtcc tcggcggcgc gaacgccctc ggcgggacga tcaacatcat cagccggacg
     4321 gccggaacga agccgttcac cgagctcctc gtggaggggg gcgactacgc caccgggcgc
     4381 ttcgtcgcca cgcacggcgc gcggcgcggc cccctgagct actggctgaa cctctccggc
     4441 cagacgagcg gcgggacgcc cgtccccggc ggcttcacgc cgaggaccgg gacgatccag
     4501 cagaagaacc cgacgaagaa cacgccggcc gtgatcgagg acggcggccg ccgcgagaac
     4561 tccgacacga agcagctgaa cgcctgggcg aagctcggct gggagccgtc gaaggcaacg
     4621 gcgctcttcc tcaacctccg ctacttcgac cgcgacaagg gggccccgcc cgcgacggac
     4681 gccgtccagg tcttcctcaa gcgccccgcc ttctcgcagt tctcgcgcat cccgacctat
     4741 cgcgacgtcg cgttcgacct cgacggccgc cacgagctcg ccaaggggct gacgctcaag
     4801 gcgaaggcgt tccaccacga gcacgtcgac gactacgagt cgtacaagga cgagacctac
     4861 tccgagcgga tcgcgatcag ccgctacgac gacacgctca ccggcggctc gctcgtcgcg
     4921 gagtgggccg cggccccctg gagcaccctc cgcctcgccg cccacctgaa ggacgacacc
     4981 caccgcgagc gcgacgacgc ctacctcccg ttcgccgagt cggcctcgat caccggctcc
     5041 ctcgggctcg aggactcctt cacgctgtcg agccggctca gcgccgtcgc cggcgtctcg
     5101 tacgactggt tcgacgtctc gcgggccgag cggaacgtca ccgacggctc gacgggcgac
     5161 ttcgtgcggc aggaccggct cgcgacgccg aacgacgacc agctcaaccc gatggcgggg
     5221 ctcgtctggc ggagcgcctc cggcaccgtc gtcaacgcct ccctcgcgcg caagagccgt
     5281 ttcccgaccc tctcccagct ctactccacg aagagcggca acacggccct cgcctcggag
     5341 acgagcgtca acgccacggc gggcgtctcg caccccctcg gcgcgaacgt ccgcgtcgaa
     5401 gccggcgcct tcctctacga cgtgagcgac atgatcacgc ggaacggctc gagcgccacg
     5461 aacctctacc tgaacgtcgg gaaggtgcgg atggcggggt tcgagacgct cctcgaggtg
     5521 acgccgctcc ccggcctcac gctccgcgcc gactacacgt tcgttgacgc gaaggagcga
     5581 accgccgttc gcgtcaccga cgacgtcacg aacgtccccc ggcacaaggc gggcttcctc
     5641 gcctcctggg cggccaccac cggcacgcgc gtcgacctcg acgccgcctg gctcggctcg
     5701 atgtactcga gcctcccgtc cgtcgcctac ccgaaagacc cggtcaagga gagcgacggc
     5761 tacgtcctcc tcggcggcaa ggtgacgcag gaggtcctcg acggcctccg tctctgggtc
     5821 tccggccgca acctcctcga cgaggactac gagagcgagg tcggcttccc cggcgtcggg
     5881 aggagcttct ccttcggggc ctcggcgacg ttctga
//
LOCUS       DAOONL010000181         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_16458_length_5824_cov_10.369388, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000181 DAOONL010000000
VERSION     DAOONL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16458_length_5824_cov_10.369388"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="PLP50_16100"
     CDS             complement(<1..1451)
                     /locus_tag="PLP50_16100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA53117.1"
                     /translation="MTDARLVKKQELLASLALAPTEDRPRFLAENCGDDPGLRAEVEA
                     LLPYLGAPTPSPAGDLEAALEEKLAERFVGKAFGPYTVQRLLGRGGFSRVFLATRSFE
                     GQERTYAIKVPLWGSFAEEVARYRLEIAVLSQLRHPNIAGLVDVVPGPDDRPLLVMDY
                     VDGARPITLAAAETSLPVRQRIELFLKVLSALSAAHERGVIHCDLSAGNVVLRADGEP
                     VIIDFGIAKVSWERSGTVTVGPRPLTYEYASPEQYRGEPLTVRTDIYSAGVLLYELLA
                     GALPYDVDRKDTEACRRAVCDVPAERASQRALRSSGPAPGPVRSVAKQLRGDLDAILG
                     KALEKDPARRYESANSFAEDLRAHLELRPVVAKAPSGFERSWRWARRHPAISATAVLA
                     VILSVGFGTFHVVRLEREQARARKIAAAFRDVLRGFDPAAVAKNPVTVRDLLDQAAAK
                     AEDQLKDEPLVRGAVLETLGTTYSSLGDLDRAE"
     gene            complement(1448..2023)
                     /locus_tag="PLP50_16105"
     CDS             complement(1448..2023)
                     /locus_tag="PLP50_16105"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HPA53118.1"
                     /translation="MGVGPVAPQQRVTELLRRWSTGDETAFEEAVALVYEDLYRIARR
                     KMANERPGHTLQPTALVNEAIAGIMSWNGYTYADRAHFLRAACFAMRRVLVDYARKRA
                     RAVQAEVSLAEGDVEIPGETGASVVSATALSQALDRLAAEDPKTAEVASLRLFADLTL
                     QQIAEVTETTVPTVHRKWVYAKAFLRKELSS"
     gene            2689..3603
                     /locus_tag="PLP50_16110"
     CDS             2689..3603
                     /locus_tag="PLP50_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPA53119.1"
                     /translation="MALPKSLESWDRRFRDFCDYAVTALGHSAATLHGYRHAYRDFRR
                     YVEESGAPLPDAFLDVEGWVRRNRRRGLGPVTVNTYWRQLRPFFNDLERRDGVENPFR
                     RMKAPSQPSRIAKAFKPADCRKILAAAESFPWPTSFQRTRAVAVIATILYAGLRRGEL
                     LRLTYGDVSLDDGTIRIIRGKGRGGGKDRTAYIAPELRRILEEYVRERRKRNIVGPGF
                     FSSERTGQGISLATLRRIHKAVRRASGIAFSLHGLRHSFVTMLLRSGVPLHVARELAG
                     HTSITTTAGYLRVWDEDKRDQIRKVSFR"
     gene            complement(3631..4593)
                     /locus_tag="PLP50_16115"
     CDS             complement(3631..4593)
                     /locus_tag="PLP50_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPA53120.1"
                     /translation="MPVAQPFTFPSLSSFEEAFEEALVASAAAEGLSPPTVAGVRYSL
                     RRLTSFIRERRLERAFLTGQLPEQARALEMWLGWLRAGGANHTTVNYYWRMLHAGLKR
                     LGARTGMAEPTQFLPVPRPGRPLPRFLSRTDLEAVLRAAANYQWPRGPFERHRSVAII
                     AMMGLGGLRRGEVLRLEVRDIDLTQGAVTVRHGKGRSGGKDRVVFMPPGLRTALSSYL
                     DVRAARPGSTGRLFISTRGTRPIGVTTIRRLCRTLQEISGVAVAPHMLRHTCATLLRQ
                     AGIPDRLAMEQLGHASLGVLQRYSHVTDEERRQIVGRLDIDLLT"
     gene            complement(4722..>5824)
                     /locus_tag="PLP50_16120"
     CDS             complement(4722..>5824)
                     /locus_tag="PLP50_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53121.1"
                     /translation="ARISGQLRVVSLDFTRPERLPSWNPLLPMTGVDPGRQVDLLVSV
                     LRRLYSAERASSWAWGVKVEEIVRMALRAAIESEVPVTLADLERFLLDPRYRKEALAT
                     AGPEPTNYFLHRFGPQEQVYVSAVVNKLSPFLGSAAVRRFLGGGGEPVDLLDLPDRPV
                     ALIVNLARGVLGAAADVLGRLLLNTLLLSALRRTGSVPETRRHFALLVDEAHTFAGTE
                     GGLEDLLVTGRKFHFALTLASQGLSLFPAHLRPLLLGNTARQFYFRLPYAEARLLGPD
                     ILEPLGNVGREAVRPYDDLSDPLLDPREELTARMRELTDLPRGACYWALRGRRFKARR
                     IRVDRPENPPARLSEESMARMAASEIHFTTSS"
BASE COUNT          932 a         1920 c         2050 g          922 t
ORIGIN      
        1 ttctcggccc gatcgaggtc cccgagcgac gagtacgtcg tcccgagcgt ctcgaggacc
       61 gcccctcgga cgagcggctc gtccttcagc tggtcttccg cctttgccgc ggcctgatcc
      121 agcaggtctc gcacggtcac cggattcttc gccacggccg ccgggtcgaa tccccgcagg
      181 acgtcccgaa acgccgctgc gatcttccgg gcacgcgcct gctcccgttc gaggcggacg
      241 acgtggaagg tgccgaagcc gacggagagg atgaccgcga gaaccgccgt ggccgagatc
      301 gccgggtggc gcctcgccca gcgccaggag cgctcgaagc cgctcggcgc cttcgccacg
      361 acggggcgga gctccagatg ggcccggagg tcttctgcaa aggaattcgc ggactcgtat
      421 cgacgggcgg ggtctttctc gagcgccttg cccagaatcg cgtcgaggtc cccgcggagt
      481 tgcttcgcga cggaccggac cggccctggg gcgggtcccg aagaccgaag cgcccgctgg
      541 ctcgcacgtt cggccggcac gtcgcagacc gcgcgccggc acgcctcggt gtccttccga
      601 tcgacgtcgt agggcagggc accagcgagg agttcgtaga gaaggacgcc tgcggagtag
      661 atgtcggtgc ggacggtgag aggctctccg cggtactgct cagggctcgc gtactcgtac
      721 gtgagcggcc ggggcccgac cgtgacggtg ccggagcgct cccacgagac cttcgcgatg
      781 ccgaagtcga tgatgacggg ttcgccgtcc gccctcagca cgacgtttcc agccgagaga
      841 tcgcagtgga tgacgccgcg ctcgtgcgcg gcgctgagcg ccgagaggac cttcaggaag
      901 agctcgatcc tctgtcggac cggtaggctg gtctcggcgg cggcgagcgt gatcggccgg
      961 gcaccgtcca cgtagtccat gacgaggagc ggccggtcgt cagggccagg aaccacgtcg
     1021 accaggcccg cgatgttcgg gtgacggagc tggctcagga cggcgatctc gaggcggtag
     1081 cgcgcgacct cctcggcgaa ggagccccag agaggcacct tgatcgcgta cgtccgctcc
     1141 tgcccctcga acgaccgggt cgccaggaac acgcgggaga acccgcctct gccgaggagc
     1201 cgctggacgg tgtagggccc gaaggccttt cccacgaacc tttcggcgag tttctcctcg
     1261 agggcagcct cgaggtctcc tgccggggat ggcgtcgggg ctccgaggta ggggagaagg
     1321 gcctctactt cggcccggag acccggatcg tcaccgcagt tctcggcgag gaatcggggt
     1381 cggtcctcgg tcggcgcgag agccagcgaa gccaggagct cctgcttttt cacgaggcgg
     1441 gcgtcggtca cgaggagagc tcctttcgaa ggaacgcctt ggcgtagacc cacttcctgt
     1501 ggacggtcgg gacggtcgtt tcggtgacct cggcgatctg ctggagcgtg aggtccgcaa
     1561 acagccgaag cgaagcgact tcggcggtct tcgggtcctc ggcggcgagc cggtcgagcg
     1621 cctggctcag cgcggtggcg gagacgacgg aggctccggt ctccccgggg atttcgacat
     1681 caccctcggc gagcgagacc tccgcctgca ccgccctggc acgcttccgg gcgtagtcga
     1741 caaggacgcg ccgcatggcg aaacacgccg cccggaggaa gtgggcgcgg tcggcgtagg
     1801 tgtagccgtt ccagctcatg atcccggcga tggcttcgtt cacgagggcg gtcggctgga
     1861 gggtgtgtcc ggggcgctcg ttggccatct tccgtctggc gatccggtag aggtcctcgt
     1921 agaccagcgc gaccgcctcc tcgaaggcgg tctcgtcacc ggtgctccac cgccggagca
     1981 gttcagtcac acgctgctgt ggggcgaccg ggccgacgcc catggttgtt cgagctacct
     2041 ccgccgcaaa gcgcgccgat tagaccagat tcctcgatca ggccgggttc agccgccgga
     2101 gcaccggggc gccggcgtcg ctcggcattc cgaggaatct gcctattcgt ggcgtttaag
     2161 gtaagtgtat ttgccttagc gaacccgtgc attcgttcag gatcgccgtt cgtcgactcg
     2221 gtactgtccc gctccagccc ttgcgtgccg ctcaggtccc gcgtatcaag gagatgcgtc
     2281 gttccttcac cttctccgtc gaatgagcac ttcgcgcacg cgcgaaggct cgctacgagc
     2341 gctgaaggtg accgaaacgg cttgtgcatg aggagtagtc gcgctggcgg ttggagcttc
     2401 cgcctgtcac gcggaaggtc gcgagttcga ccctcgtcat ccccgccctt tccggtcacc
     2461 gcgcagcagg aagccacgaa gcgtggtttc gactgccaca gcgttccgtt ccgtctgagc
     2521 ctccgcgcgt ttcgcgtgga ggttccgtcg gtctcattcg cggagatggc ggagggaagc
     2581 gcttccttcc ccccaatccc catgttcccc tccacttccc agcgagcctc cgcgtacgag
     2641 gacatcgacg actcctcccg gaatcgcttc gcttctggac gccatcgcgt ggcacttccg
     2701 aaatctctcg aatcgtggga tcgccgcttc cgggacttct gcgactacgc cgtcaccgcg
     2761 ctcggtcaca gcgccgcgac gctccacgga taccggcacg cctaccggga cttccggcgg
     2821 tacgtcgagg agagcggcgc gccgctcccg gacgcattcc tcgatgtcga gggatgggtc
     2881 cgccggaacc ggcgacgtgg actcggccct gtcaccgtga acacctactg gcggcagctc
     2941 cggccgttct tcaatgacct cgagcggcgc gacggcgtcg agaatccctt ccgacggatg
     3001 aaggcaccga gccagccttc ccgcatcgcg aaggcgttca agcctgcgga ctgtcgaaag
     3061 atcctggcag ccgcagagag cttcccgtgg ccgacgtcct tccagcgcac gcgcgcggtc
     3121 gccgtgatcg cgacgatcct ctatgcggga cttcgccggg gcgagttgct gaggctcacg
     3181 tacggcgacg tgagcctcga cgacggcacg atccggatca tccgtggcaa ggggcgaggc
     3241 ggtgggaagg accggaccgc gtacatcgcc ccggagctca ggcggatcct cgaggagtac
     3301 gtccgggagc gtcgcaagcg gaacatcgtc gggccgggct tcttctcctc cgagcggacc
     3361 ggccagggga tctccctggc cacgttgcgg cggattcaca aggccgtccg gcgagcgtcc
     3421 gggattgcct tctcccttca cggactccgc cactcgttcg tgaccatgct gctccgctcc
     3481 ggcgtgccgc tgcacgttgc ccgcgagctg gccggacata cgagcattac tacgacggcg
     3541 gggtacctgc gagtgtggga cgaagacaag cgtgaccaga tccgcaaggt ctccttccgg
     3601 tagaaccggc cggcccccgg ggccggctgg tcaggtcagg aggtcgatgt cgagtcgacc
     3661 gacgatctgt cgccgctctt cgtcggtgac gtgcgagtac cgctgcagca cgccgagact
     3721 cgcgtgcccg agctgctcca tggcgagacg atccgggata ccggcctgcc gaaggagggt
     3781 cgcgcaggta tgccgcagca tgtggggcgc cacagccacc ccggaaatct cctgcagcgt
     3841 ccggcagagg cgacggatcg tggtcacacc gatggggcga gtaccgcgcg tcgaaatgaa
     3901 caggcgaccc gtggagcccg gtcgcgctgc cctcacgtcc aggtagctcg acagggcggt
     3961 tcgaagccct ggcggcatga agaccacacg gtccttgcca ccgctgcgac ccttgccatg
     4021 ccggaccgtg accgctccct gagtgaggtc gatgtcccgc acttccagcc ggagtacctc
     4081 tcctcggcga agacccccga gtcccatcat cgcgatgatg gccacgctcc ggtggcgctc
     4141 gaaggggccg cgaggccatt ggtagttggc ggctgccctg agcacggctt cgaggtccgt
     4201 tctcgagaga aaccggggaa gagggcgtcc gggccgggga acggggagga actgcgtggg
     4261 ttccgccatg ccagtcctcg ctccgagccg cttcagcccg gcatggagca ttcgccagta
     4321 gtagttgacg gtcgtgtggt tggcgccgcc agcgcgaagc catccgagcc acatttcgag
     4381 ggcacgcgcc tgctccggga gctgtcccgt taggaaagcg cgttcgaggc ggcgctctcg
     4441 aatgaaagag gtcagccgac ggaggctgta ccggactccg gcgacagtcg gcggcgaaag
     4501 gccctccgct gcagccgagg ccacgagcgc ctcttcgaac gcctcctcga agctcgagag
     4561 agacgggaag gtgaagggct gcgcgacggg cacgacgaaa tcaaggtgac gagcgtttcg
     4621 agacacggcc tttccggccg actccggcca acgcgggccc gccaggcggg caccacggcc
     4681 ggagccggga gtcagaagct gtccgcggtt gtcgctcatg gtcaggatga ggtagtgaag
     4741 tggatctcgc tcgccgccat ccgggccatc gattcctcgg acagccgagc cggcgggttc
     4801 tccggccggt ccactcggat gcgtcgggcc ttgaaccgcc ggccgcgcag cgcccagtag
     4861 cacgcgcccc gcgggaggtc ggtgagctcg cgcattcgag ctgtgagctc ttcacgggga
     4921 tcgagtaggg gatccgagag gtcgtcgtac gggcggaccg cttcccgccc gacgttcccg
     4981 agcggctcga ggatgtccgg cccgaggagg cgcgcctctg cgtaggggag ccggaagtag
     5041 aactgccggg ccgtattccc gaggagcagc ggtcgaagat gcgcagggaa gagcgaaagg
     5101 ccctgagagg cgagagtgag ggcgaagtgg aacttccgcc ccgtgacgag gaggtcctcg
     5161 agcccgcctt cggtcccggc gaaggtgtgc gcctcgtcca cgaggagggc gaagtgccgc
     5221 cgggtctctg ggaccgagcc ggtgcgccgg agggctgaga ggagcagcgt gttcaggagg
     5281 agtcggccga ggacgtcggc ggcggcgccg agtaccccgc gggcgaggtt cacgatcagg
     5341 gccacgggcc ggtccgggag gtcgaggagg tccacgggct cgcccccgcc cccgaggaat
     5401 cgccgcacgg cggccgaacc gaggaacggg gagagcttgt tgacgaccgc cgagacgtac
     5461 acctgctcct gggggccaaa ccggtgcagg aaatagttcg tgggctccgg cccagccgtc
     5521 gcgagggcct cctttcggta gcgcgggtcg agcaggaagc gttccaggtc ggcgagcgtc
     5581 acaggaactt cgctctcgat ggccgcgcga agcgccatgc ggacgatctc ctcgaccttt
     5641 acgccccagg cccaggacga ggcgcgctcg gcgctgtaga gccgtcgaag gaccgagacg
     5701 aggaggtcca cctggcgtcc ggggtctacg ccggtcatcg ggaggagcgg gttccacgac
     5761 ggcaggcgtt ccggccgggt gaagtcgagg gagacgacgc gaagctgacc ggagatccgc
     5821 gccc
//
LOCUS       DAOONL010000182         5792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_16587_length_5792_cov_16.200279, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000182 DAOONL010000000
VERSION     DAOONL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16587_length_5792_cov_16.200279"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..729
                     /locus_tag="PLP50_16125"
     CDS             265..729
                     /locus_tag="PLP50_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53122.1"
                     /translation="MHDTIPRRTTTPCAATVQLDDGSRLDVTFWLLPDSHRERGVTPM
                     RVLLEEDRRFLPIGLAGGGSCLLSRDAIVSVAIEADGPGAEPTPPEGAALDLVTLHLV
                     SGEEVSGVLTTTALEGHGRMSDVFNLAGRFVPVGVGAQLVFVATSRIARVSF"
     gene            1338..2357
                     /locus_tag="PLP50_16130"
                     /pseudo
     CDS             1338..2357
                     /locus_tag="PLP50_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
     gene            complement(2578..3207)
                     /locus_tag="PLP50_16135"
     CDS             complement(2578..3207)
                     /locus_tag="PLP50_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53123.1"
                     /translation="MKRLGSLAVSLLVLAVALLPAGCATLDQLSSTLVNLKRLEFRIG
                     QVTGFRLAGIDVGRASALSQLTPFDAMALAGAYASRRLPAELVVEVEAVNPNDGTAGT
                     PRTTSTLTSLECRLLVDGRPTVTGNIDRAFEIPGTGQATVVPIRLSLDLYEFFGEKRY
                     ADLIDLALALGGESRDISRISLDAQPTVTTPLGNITYPGRITISGSRLR"
     gene            complement(3329..3691)
                     /gene="ssrA"
                     /locus_tag="PLP50_16140"
     tmRNA           complement(3329..3691)
                     /gene="ssrA"
                     /locus_tag="PLP50_16140"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3761..4135
                     /gene="cutA"
                     /locus_tag="PLP50_16145"
     CDS             3761..4135
                     /gene="cutA"
                     /locus_tag="PLP50_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPA53124.1"
                     /translation="MSNAVTLCLTTVGSEEDAARVARHLVETKAAACVSIVAPVRSIY
                     VWKGELQDEPEWLLLVKTARPAAELRDLLRAVHPYEVPELLAFPASFADEAYARWVVE
                     GSGGGAEAAQSGTLALDAPVGR"
     gene            4419..5714
                     /locus_tag="PLP50_16150"
     CDS             4419..5714
                     /locus_tag="PLP50_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPA53125.1"
                     /translation="MKRFPAVVVTGARQVGKSTLLRHALRDAADFVVFDPVVDVENAR
                     RDPELFLENHRTPLVLDEVHYAPELLPSLERRVDRDRSPGRYVLTGSQQWGVLATVAE
                     SLAGRAAFLDLDGFSLRESLEATAEKHWLDAWLESPESVADRGVRRLGGEVALFERIW
                     RGSLPEAHFLPLDTIPDFHLAFQRTYIERDARLLAEVADWQQFGRFARLVAALTGQEL
                     NRSQLGREIGVSPRTAERWLGILRATFQWFEVPAYSGNTVKRVASKAKGYVADTGFAC
                     HALAISTPNAVASHPAWGALFETAVYAEIRKAVSLMWPRPFAHHWRTGAGAEIDLLLE
                     RDGTFFPIEVKGATRPSRGDPTGITAFRKTYPKLRVARGLVVTPCEKLEPLSESDWAM
                     PWDAVAPAPAGPARRDAKRRGPSPKGEAAGRLGGAEGDT"
BASE COUNT          823 a         2157 c         1985 g          827 t
ORIGIN      
        1 cggagagcca cgtcttcccc tcggcgtcgg ccgccgccac gaggagcgag gagccgccgg
       61 gcgcgacgaa ggtggcggtc tgagccgagg catgggccgg caggagggcc agcagcgccg
      121 cgagggcgag gtacgggagg agggcgggcc ggatcacgcc ccgcgattat ctacgcgccg
      181 gcgctcgcgc cgccggacgg atcggaacgg aggcggcgct gcggctgatt cgcctaagat
      241 cggctcgagc ccccggaggt tcgcatgcac gacaccatcc ctcgccggac gacgaccccg
      301 tgcgccgcca ccgtccagct cgacgacgga agccgactcg acgtgacgtt ctggctcctc
      361 cccgattcgc accgcgagcg gggcgtgacg ccgatgcgcg ttctcctcga ggaggaccgg
      421 cgcttcctgc cgatcggcct cgccggcggc gggagctgtc tcctctcgcg cgacgcgatc
      481 gtcagcgtcg cgatcgaggc ggacgggccc ggggccgagc cgacgccgcc ggagggggcc
      541 gcgctcgacc tcgtgacgct ccacctcgtc tccggcgagg aggtctccgg ggtcctgacc
      601 acgaccgcgc tcgagggaca cggccggatg tccgacgtct tcaacctcgc cgggcgcttc
      661 gtcccggtgg gcgtcggcgc ccagctcgtc ttcgtggcga cctcgcgcat cgcgcgggtc
      721 tccttctgac ggagcgaccg atggcctccc gcgtcgacct cctcgtcgcg aacctcctcc
      781 agaccaccgg cgacgcgctc tacatcgtcc cgggcgagaa gctcttcatg acgcgcggcg
      841 acgttcgcgc cgtcgtcgga cgcgagccgg tcggggtgga gtcgttccgc gccgtcgtcg
      901 cggagctcgt tccgggtcgc gctcccgaag agctggccgt ctcgcggcac cggatgccct
      961 ggccgaccgg ccccgggaag gaccatgtcg agattcgatt cggccaggtg ggcgcgacgc
     1021 ccgccgtgat gctctgccgc cccgaccgga tgtccgcctc ggtcgtccag cgcccgaagc
     1081 gagccgcgcc ccccgtctcc tcggtggacg tccccgaagc tcctccctcc gtcggaatgc
     1141 tcgagtcggg gccgcctcgc ctggcgccgg aggagatccc ggtcccgccc gagctcgcgg
     1201 cgccttcggc cgccttcccc ggtgggcccg ctcccgcgcc ggcccccgcg gcgccgctgg
     1261 aggagattcc ctaccccgcc ggcgccggcg ccctcgaggc cccctcgctc cgtccggtcg
     1321 ccccgaccgc gcgcccgctg gacggcatcc tcctccagat gctcgacctg aaagcctccg
     1381 acgtgcacct ctcctccggg acgcgccccg tctttcgcgt ccacggcgag atggccgccc
     1441 aggccgatct cgactccctc tcctccgacg cggtcgagag gctcctcgcg ccgatcttcc
     1501 ccgagaagaa ccggaccgag ttcatggagc ggcacgacac cgacttcgcc tacgagatcc
     1561 cgggcgtcgc ccgcttccgc gtcaacgtct tccgcgaccg gcacggcgtc ggcgccgtct
     1621 tccgccagat cccgatcgag atcctcacgg cgaagcagct gaacctgccc gacgcggtcg
     1681 tgaggctggg cgacctctcg aaggggctcg tcctcgtcac cgggccgacc ggctcgggca
     1741 agtcgacgac gctcgccgcg ctcgtcgact acgtcaacga gacgcgcacc gaccacatca
     1801 tcacgatcga ggacccggtc gagttcgtcc accgcaacaa gaagtgcctc gtcaaccagc
     1861 gcgagatcgg gagccacacc cgctcgttca aggacgcgct ccgagccgcg ctccgcgagg
     1921 accccgacat cgtcctcgtc ggcgagatgc gcgacctcga gacgatcgcc atcgccatcg
     1981 agaccgccga gacgggccac ctcgtcttcg gcacgctcca caccacgacg gcgatgtcga
     2041 ccgtcgaccg gatcgtcgac cagttccccg ccgaccggca gcagcagatc cgggtcatgc
     2101 tcgccgactc gctgaagggg gtcgtcgccc agacgctcct ccggaaggtc ggcggcgggc
     2161 gcgtcgccgc cctcgagatc ctcctgacga acaacgccgt cgcgaacctc atccgcgacg
     2221 gcaagacgtt ccagctcccg tcggtcctcc agacctcgaa ggcgcagggg atgcgcctcc
     2281 tgaacgactc gctcctcgac ctcgtcaagc aggggctcgt cgagccgcgg gaggcctaca
     2341 tgaagtcgat cgacaagagc gccctcctcg gcgccttccg gtcgaacaac atcccgctcc
     2401 cgacggagac cccggcggag gcggcgcccg ccgcggcgcc cgcggcggca ccggcccccg
     2461 gcggagcgaa gccgggtggc gcttcgcctg ccggcggagc gaagcccggg ggcgcttcgg
     2521 cgccggcgag gcggccggcg ggctgaaggc gcgcggggcc gcggatcgct gccggcgtca
     2581 gcggagccgg ctgccggaga tcgtgatgcg gcccgggtac gtgatgttcc cgagcggcgt
     2641 cgtcacggtc ggctgggcgt cgagggaaat gcgggagatg tcgcgactct ccccgccgag
     2701 ggcgagcgcg aggtcgatca ggtcggcgta gcgcttctcg ccgaagaact cgtagaggtc
     2761 gagcgagagg cggatcggaa cgaccgtcgc ctgccccgtt cccgggatct cgaaagccct
     2821 gtcgatgttc cccgtcaccg tcggccggcc gtcgacgagg aggcggcact ccaggctggt
     2881 gagcgtgctc gtcgtcctcg gcgtccccgc ggtgccgtcg ttcgggttca cggcctcgac
     2941 ctcgacgacg agctcggccg gcagccgccg cgaggcgtat gcccccgcga gcgccatggc
     3001 gtcgaacggc gtgagctgcg agagcgccga cgccctcccg acgtcgatcc ccgcgagccg
     3061 gaagcccgtg acctggccga tccggaactc gagccgcttc aggttcacga gcgtcgaaga
     3121 cagctggtcg agcgtcgcgc agccggccgg gaggagggcg accgcgagga cgaggagaga
     3181 gaccgcgagg gagccgagcc gcttcatgcc ggaatcgtcc cacagggaag gccgggtcgc
     3241 ggcccccgcg gcgggacgga cgttgccgtt cgccccgacg gaaaggcccg ccgccggctt
     3301 ccccggcggc gggccgcgac tcccgagatg gtggaggcgg ggggagtcga acccccgtcc
     3361 gaagcgaccg tacgtcgagg ttctccacgc tcagtcccga cttcttttct cgtcgccgcg
     3421 cttccgggtc gggaccacca ggcgctgcga ccaacctgga acgtctcgga cctcgccttc
     3481 aggtggcggg cttcggtccc agcctggttc tctcaacagg gcggcggagc gctccaggct
     3541 ggctccccgt gccccgcccg cggttaatta agccgcgaga gcgagttcgc tgtcgttggc
     3601 agcttttgtt taccgtcgtt tttacgggac aaacggcatg cccggcgtgc tcctcgatcg
     3661 cgagatgccc cgtcgaaacc gggtcgcccc cgtgatcggc tcgcatttta tcgcgggggt
     3721 caagtgtgcc tgcgcccgcc cgctggtagc gttccgcgcc atgagcaacg ccgttacgct
     3781 ctgcctgacg accgtcggct ccgaggagga cgccgcccgc gtcgcccgcc acctcgtcga
     3841 gacgaaggcc gccgcctgcg tctcgatcgt cgcgccggtc cggtcgatct acgtctggaa
     3901 gggagagctg caggacgagc cggagtggct tctcctcgta aagaccgcgc gccccgccgc
     3961 cgagctccgc gacctcctcc gcgccgtcca cccctacgag gtcccggagc tcctggcctt
     4021 ccccgcgagc ttcgccgacg aggcttatgc gcggtgggtg gtggagggga gcgggggagg
     4081 agccgaagcc gctcagtcag gaacgctcgc gctcgacgct cccgtcggcc ggtaggaacc
     4141 atgccggtac ggcagatttc gaccgagtgc aaggaatgtc gcgacggaat tggcgcagga
     4201 gcagattccg ctggcgacgc accgttgtcg tcgaggacct ggcccgggag gatcatccgt
     4261 ggaagcgccg ctacctcgcg gggccggccc gcgatcgcga ccagagggaa cgcgcctggc
     4321 ccgacggcga ttcgaatacc ttgcaaaaca ggcgcgttgc gatcaaaacg caaggtatgt
     4381 tcctgcttcg aagtgcggag cagcgcctct cggctctcat gaagcgcttc cccgccgtcg
     4441 tcgtcaccgg ggcgcggcag gtgggcaaga gcacgctcct tcgacatgct ctgcgcgacg
     4501 cggccgactt cgtcgtcttc gaccccgtcg tcgacgtgga gaacgcccgc cgcgacccgg
     4561 agctcttcct cgaaaaccac cggacgcccc tcgtcctcga cgaggtgcat tacgcgcccg
     4621 agcttctccc ctcgctcgag cggcgcgtcg accgggacag gagcccgggg cgctacgtcc
     4681 tcaccgggtc gcagcagtgg ggcgttctcg ccaccgtcgc cgagagcctc gcgggccgcg
     4741 cggccttcct cgacctcgac ggcttctcac tccgggagtc cctcgaggcg acggccgaga
     4801 agcactggct cgacgcgtgg ctggagtctc ccgagtccgt cgccgatcga ggcgtccgac
     4861 ggctcggcgg agaggtcgcc ctcttcgagc gcatctggag gggctccctg ccggaggcgc
     4921 acttcctccc cctcgacacg atccccgact tccacctcgc cttccagagg acgtacatcg
     4981 agcgcgacgc acgcctcctc gccgaggtgg ccgactggca gcagttcggg aggttcgccc
     5041 ggctcgtcgc ggcgctcacc ggccaggagc tgaaccgcag ccagctcggg cgcgaaatcg
     5101 gcgtctcgcc tcggacggcc gagcggtggc tcggaatcct gagggcgacg ttccagtggt
     5161 tcgaggtccc cgcctactcc gggaacaccg tgaagagggt cgcgtcgaag gcgaagggct
     5221 acgtcgccga caccggtttc gcctgccacg cgctcgccat ctcgacgccg aacgccgtcg
     5281 cgagccaccc ggcctggggc gccctgttcg agaccgccgt ctacgcggag atccgcaagg
     5341 cggtttcact gatgtggcct cgccccttcg cccaccactg gcgcacgggt gcgggcgccg
     5401 agatcgatct ccttctcgag cgggacggga cgttcttccc gatcgaggtc aagggcgcga
     5461 cccgcccctc ccgcggcgac cccacgggga tcaccgcgtt ccggaagacc tacccgaagc
     5521 tgagggtggc tcgaggcctc gtcgtcactc cgtgcgagaa gctcgagccc ctctcggaga
     5581 gcgactgggc gatgccgtgg gacgcggtgg cgcccgctcc tgcgggtccc gcacgccgcg
     5641 acgcgaaacg ccgaggccct tccccgaagg gcgaggcggc gggcagactc ggaggagcgg
     5701 agggcgacac gtgaaggccg accagctgaa agcaacgtgg ctcctcctcg agcgcccctg
     5761 gctcgagatg gtcgtgcgcc gcggcccggc gc
//
LOCUS       DAOONL010000183         5657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_17138_length_5657_cov_11.621742, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000183 DAOONL010000000
VERSION     DAOONL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17138_length_5657_cov_11.621742"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLP50_16155"
     CDS             <1..611
                     /locus_tag="PLP50_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HPA53126.1"
                     /translation="IRIVRRLAALYPDETASRLKRAVIEADGAIARAEADKRIMQCET
                     GREAMVAEEVGRVTAAIAKAEAQLKVQQARIEQVRLQLEADVVAPARADMEKRQAEAR
                     GKAAKIVEDGKATAAVLTQMIETWKAAGPNARDIFLMQKLQSVMSSLVETIGSVKVDR
                     VAYLPASAKVAQGVQLVEQVKAAFGVDLAQVVGRLGAPEEKG"
     gene            complement(628..2100)
                     /locus_tag="PLP50_16160"
     CDS             complement(628..2100)
                     /locus_tag="PLP50_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53127.1"
                     /translation="MSERSGEGPGGEAGWPWIRSWALLLAAAGSVAFLSAAALRRLNF
                     DEPLALRAGFLSLSGIPAEPGFAMPFTVLLGALGLGVADPGLLFVLARLLVAGGVLAA
                     LVFAFRGAGRAPGLVPVALFATLANATFFVHGLEFRYDAAILVLLVAALPCLVRGSDR
                     ALVALGVLAGLLATHHLKGAFFAAALLGFAALRGWPRGIPRVAAGFLAAAGAWLLVAG
                     AAGVLDDVVGLYRSFSDVAIGSERWGPWATLKDSILRDAAWWGLGAAALFWSIASLAG
                     LSSEELRRSPDLWALALAGASVSFLYVHPHPFAYMLALPAPFVGFLAARRLVAARPPA
                     LAAAGFAAALALGAQGIVEPVPFAAHVSSFQAPMGPQVEALRWLKARSSPGEKVLDPS
                     GLAYFLPPCTTQWYLDSLFERRARHGAWMTDLPRTDLAGCPWMVLTYRLNMLPRKFRL
                     RLPAEYGPAHPGVALHRRDGRWGTPGFERPAAPVSLENFW"
     gene            2243..2446
                     /locus_tag="PLP50_16165"
     CDS             2243..2446
                     /locus_tag="PLP50_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53128.1"
                     /translation="MLVLLLLIVVPRPAAAYIDPGTGSLVIQALIAGLVTASVIFRRA
                     FGRVFGGILRLFGRRQPPPPGRT"
     gene            2458..3834
                     /locus_tag="PLP50_16170"
     CDS             2458..3834
                     /locus_tag="PLP50_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPA53129.1"
                     /translation="MSERREPSSYRDPAGFLFFRDGVLLRHVAPAGAADYDALLSSGL
                     YEALALEGLLVPHEEVEPPEGHEDAHRVLRPRVIPFVSYPYEWTFGERRDAGLLTLSV
                     QRRALERGLSLKDASAYNVQFLDGRPVFIDTLSFERYPEGRPWVAYRQFCEHFLAPLA
                     LEATGDHRLGRLFSSFPDGVPLDLASRLLPFRTKLRPGLALHLHLHSWGQGRYSSGPA
                     RPSPTVSKGRLLDLVADLEACLAGLRLPRRTSTWSDYYAGTNYSPEALESKKEFVRAA
                     LGEVRPSVVWDLGANTGLFSRLASEAGAFTVAMDGDPLASEGHYRDVVGRGERRVLPL
                     VVDLSSPSPSLGWDGRERRSLFERGPADLALALALVHHLAIGANVPLGSIASSWARMA
                     EWLVVEFVPKSDSQVQRMLRSREDVFPGYTADGFEAAFGREFEVCRRAGVAGSERSLY
                     LLRRRSPA"
     gene            3831..5483
                     /locus_tag="PLP50_16175"
     CDS             3831..5483
                     /locus_tag="PLP50_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPA53130.1"
                     /translation="MTPRTLARLYPFVFSAVPALAFFSTNRGHQLLSSLLYPLLVSLA
                     VAAVATALARAAARDPHAGAFKAFVLVGWFFAAGQIGELLPPEVWVFGYSVATSLPSQ
                     AALAATALLLLRVDLGAQGEKVTQALALFSAVLAGMNLVSIALDSRPGTGRRPAAAAR
                     PVRTLPSRLPPAASLPDVVVVVLDGYGRGDVLRETYGFDNSPFLDGLRRRGFFVASRA
                     LANYPQTGLSLASALNLDYIPALLPDVRPRSTSRTPLTDLIQQNRVLDLLRAAGYRST
                     SFANGYRIAELRSVDRFVTDGPSLDPFLRAFLALTPLRALEPAAHQRPQGEVHRDAVR
                     FALEHGAEPLAEQRPVLVFAHVICPHPPFVFRADGGVPDDAKLDRYLLHDGDHLKPDD
                     PGRTEWYRRRYVEQVRFLNEGVLRLVDAVDATKGRPSAVLILGDHGPGSGLFWEDAAK
                     TDLTERLGILLACRLPGTGHRGLYDTITPVNAFRALLREALAADLPPLPDRSYFATWK
                     APFVPLEVETAVDGSVRLVDDARRRSAAAPVSLSSSPAPTSP"
     gene            complement(5452..>5657)
                     /locus_tag="PLP50_16180"
     CDS             complement(5452..>5657)
                     /locus_tag="PLP50_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53131.1"
                     /translation="YIRPDEKKVPSAFLGIGVRIEDDVLVTEEGHRVLTAAAPKSVEA
                     MCDRGEKSSVRRPPTARSARARS"
BASE COUNT          756 a         2180 c         1958 g          763 t
ORIGIN      
        1 cgatccgcat cgtccgccgc ctcgccgcgc tctaccccga cgagaccgcc tcgcgcctca
       61 agcgcgccgt gatcgaggcg gacggggcca tcgcccgcgc cgaggccgac aagcggatca
      121 tgcagtgcga gacgggccgc gaggcgatgg tcgccgagga ggtcgggcgc gtgacggccg
      181 cgatcgccaa ggccgaggcc cagctcaagg tccagcaggc gcgcatcgag caggtccgcc
      241 tccagctcga ggccgacgtc gtcgccccgg cccgggccga catggagaag aggcaggccg
      301 aggcgcgcgg caaggccgcc aagatcgtcg aggacggcaa ggccacggcg gcggttctca
      361 cccagatgat cgagacctgg aaggccgccg gcccgaacgc ccgcgacatc ttcctcatgc
      421 agaagctcca gagcgtcatg tcgtctctcg tcgagacgat cgggagcgtc aaggtcgacc
      481 gcgtcgccta cctccccgcc tccgcgaagg tcgcgcaggg ggtccagctc gtcgagcagg
      541 tcaaggccgc gttcggcgtc gacctcgcgc aggtcgtcgg aaggctcggg gcgccggagg
      601 agaagggctg accggcggcg gcctgctcta ccagaagttc tcgaggctca cgggagccgc
      661 cgggcgttcg aagcccggcg tgccccagcg gccgtccctg cggtggaggg cgaccccggg
      721 gtgcgccgga ccgtactccg ccgggagccg cagccggaac tttcgcggga gcatgttcag
      781 ccggtacgtg aggaccatcc acggacagcc ggcgaggtcg gtgcgcggca ggtccgtcat
      841 ccacgcgccg tgccgggcgc gccgctcgaa cagcgagtcg aggtaccact gggtcgtgca
      901 gggcgggagg aagtaggcga ggccggacgg gtcgaggacc ttctctccgg gcgacgaccg
      961 ggccttcagc cagcggagcg cttcgacctg ggggcccatc ggcgcctgga acgacgatac
     1021 gtgcgcggcg aacgggacgg gctcgacgat cccctgcgcg ccgagcgcca gcgcggcggc
     1081 gaagcccgcc gccgcgaggg caggggggcg cgccgcgacg agccggcggg ccgcgaggaa
     1141 accgacgaac ggcgccggga gcgcgagcat gtaggcgaac gggtgagggt gaacgtagag
     1201 gaacgacacc gaggctcccg cgagggcgag cgcccagagg tccggagaac ggcgcagctc
     1261 ctcgctcgag aggccggcga gcgaggcgat cgaccagaag agcgccgccg cgccgagccc
     1321 ccaccacgcc gcatcgcgca ggatcgagtc cttcagggtg gcccacggcc cccatctctc
     1381 cgacccgatg gccacgtcgg agaacgagcg gtagaggccg acgacgtcgt cgaggactcc
     1441 cgccgccccc gcgacgagga gccaggcccc ggccgccgcg aggaaaccgg cggcgacgcg
     1501 cgggatcccc cgtggccacc cccgaagcgc ggcgaaaccg aggagagcgg cggcgaagaa
     1561 ggcccccttc aggtggtgcg tcgcgagcag gcccgcgagg acgccgagcg cgacgagcgc
     1621 acggtcggag ccgcggacga ggcaggggag cgcggcgacg agcaggacga ggatcgcggc
     1681 gtcgtagcgg aactcgaggc cgtggacgaa gaaggtggcg ttcgcgagcg tcgcgaaaag
     1741 ggccacgggg acgaggcccg gcgcccggcc ggcgccgcgg aacgcgaaaa cgagggcagc
     1801 gaggacgccg cccgcgacga ggaggcgggc gaggacgaag aggagcccgg ggtccgccac
     1861 gccgagtccg agggcgccca gcagcacggt gaacggcatc gcaaagccgg gctccgccgg
     1921 gattcccgag agcgagagga acccagcccg aagggcgagc ggctcgtcga agttcaggcg
     1981 gcgcagggcc gcggcggaga ggaaggcgac cgagcccgcg gcagcgagga gcagggccca
     2041 gctgcggatc cagggccagc ccgcttcgcc tccgggaccc tctcctgacc gctcgctcat
     2101 cccgcccaca cctctcgtca tggtaccgtc cccgccccgt ctgctgcggc gtcccggaac
     2161 tccgttttcg tcggtacccg accgcccggg accgacggag ccgcatgcta ttctttccac
     2221 ccctatggca cgcgacgctc gggtgctcgt tctcctgctg ctgatcgtgg tgcctcgccc
     2281 tgccgcggcc tacatcgacc ccggcacggg gtccctcgtc atccaggcgc tcatcgcggg
     2341 actggtcacg gcctccgtca tcttccgcag ggctttcggc cgcgtcttcg gcggaatcct
     2401 ccgcctcttc ggccgccggc agcctccccc gcccggccgg acgtgaggtc cgggagggtg
     2461 tcggagcggc gggagccctc gtcgtaccgc gatccggccg gcttcctctt cttccgggac
     2521 ggcgtcctcc tccggcacgt cgcccccgcc ggcgcggccg actacgacgc gctcctctcc
     2581 tccgggctct acgaggccct cgcgctcgag ggcctcctgg ttccccacga ggaggtcgag
     2641 ccgcccgagg gacacgagga cgcgcaccgc gtcctgcggc cccgcgtcat ccccttcgtc
     2701 tcgtacccgt acgagtggac cttcggcgag cgccgcgacg ccggcctcct gacgctctcc
     2761 gtccagaggc gggcgctgga gcgcggcctc tcgctgaagg acgcgagcgc gtacaacgtc
     2821 cagttcctgg acggccgccc ggtcttcatc gacacgctct cgttcgagcg gtaccccgag
     2881 ggccgtccct gggtcgccta ccggcagttc tgcgagcact tcctcgcgcc gctcgcgctc
     2941 gaggcgacgg gcgaccatcg cctcgggcgg ctcttctcgt ccttcccgga cggagttccc
     3001 ctcgacctcg cctcgaggct gctcccgttc cggacgaagc tccgtcccgg cctcgcgctt
     3061 cacctgcacc tgcactcctg gggtcagggc cggtactcgt ccgggcccgc acgtccttcg
     3121 ccgacggtct cgaaaggacg gcttctcgac ctcgtcgcgg acctcgaggc gtgcctcgcg
     3181 gggctccggc tcccgcgccg gacctccacc tggtccgact actacgccgg gacgaactac
     3241 tccccggagg cgctcgagtc gaagaaggaa ttcgtgcggg cggcgctcgg cgaggtccgc
     3301 ccgtcggtcg tctgggacct cggcgcgaac accggcctct tctcgcggct cgcgtccgag
     3361 gcgggagcct tcacggtcgc catggacggc gacccgctcg cgtccgaggg ccactaccgg
     3421 gacgtcgtcg gccgcggcga gcgccgcgtc ctcccgctcg tcgtcgacct ctcgagcccg
     3481 agcccgtcgc tcggctggga cgggcgcgag cgccgctcgc tcttcgagcg cggcccggcc
     3541 gacctcgccc tggccctcgc cctcgtccat cacctcgcga tcggcgcgaa cgtgccgctc
     3601 gggtcgatcg cctcctcgtg ggcccgaatg gcggagtggc tcgtcgtcga gttcgttccg
     3661 aagtcggaca gccaggtgca gcggatgctc cggagccgcg aggacgtctt cccgggctac
     3721 accgcggacg gcttcgaggc ggcgttcggc cgggagttcg aggtttgccg gcgcgccggt
     3781 gtcgccggga gcgagcggtc cctctacctc ctccgccgcc ggtcccccgc atgacgccgc
     3841 ggacgcttgc gaggctctac ccgttcgtct tctcggccgt cccggccttg gccttcttct
     3901 cgacgaaccg gggccaccag ctcctctcgt cgctcctcta ccccctcctc gtcagcctgg
     3961 ccgtcgcggc cgtcgcgacc gccctcgcgc gcgcggcggc cagggacccc cacgccggcg
     4021 ccttcaaggc tttcgtcctc gtcgggtggt tcttcgccgc ggggcagatc ggggagctgc
     4081 tccccccgga ggtctgggtc ttcggctaca gcgtcgcgac gtcgctcccc tcgcaggcgg
     4141 ccctcgcggc cacggcgctc ctgctcctcc gcgtcgacct cggcgcgcag ggagagaagg
     4201 tcactcaggc gctcgccctc ttctccgcgg tcctcgcggg aatgaacctc gtctccatcg
     4261 cgctcgacag ccgtccgggg accggccggc gcccggcggc cgcggcgcgc cccgtgcgga
     4321 cgctcccttc ccgcctccct ccggcggcgt ccctgcccga cgtcgtcgtc gtcgtcctcg
     4381 acgggtacgg acgcggagac gtcctccgcg aaacctacgg cttcgacaac tccccgttcc
     4441 tcgacgggct gcggcggaga gggttcttcg tcgcctcgcg cgccctcgcc aactaccccc
     4501 agacggggct ctcgctcgcg agcgcgctca atctcgacta cattccggcg cttctccccg
     4561 acgtccgccc gcgttcgacg agcaggacgc ccctgacgga cctcatccag cagaaccgcg
     4621 tcctcgacct cctgcgcgcc gccgggtacc gctccacgag cttcgcgaac ggctacagga
     4681 tcgcggagct ccgctccgtc gaccgcttcg tgaccgacgg gccgtccctc gaccccttcc
     4741 tccgcgcgtt cctcgccctc acgccgctcc gggcgctcga gccggccgcg caccagcggc
     4801 cgcaggggga ggtccatcgg gacgccgtgc gattcgcgct cgagcacggc gccgagcccc
     4861 tcgccgagca gcgcccggtc ctcgtcttcg cccacgtcat ctgcccgcac cctccgttcg
     4921 tcttccgcgc cgacggcggc gtgcccgacg acgcgaagct ggaccgctac ctcctgcacg
     4981 acggggacca cctgaagccc gacgaccccg ggcgcaccga gtggtaccgg cgccgctacg
     5041 tcgagcaagt ccgcttcctg aacgagggcg tcctgaggct cgtcgacgcc gtggacgcga
     5101 cgaagggccg gccgtccgcc gtcctcatcc tcggggacca cggtccgggg tccggcctct
     5161 tctgggagga cgcggcgaag accgacctca cggagcgcct cggcattctg ctggcctgca
     5221 ggctcccggg aaccgggcac cgggggctgt acgacacgat caccccggtg aacgcgttcc
     5281 gagccctcct ccgagaggcg ctggcggcgg acctcccgcc gctgccggac cgctcctact
     5341 tcgcgacgtg gaaggcgccg ttcgtgcctc tcgaggtcga gacggccgtc gacggctccg
     5401 tccggctcgt cgacgacgca cgccggaggt ccgccgcggc cccggtctcg ctcagctcct
     5461 cgcccgcgcc gacctcgccg taggcggccg ccgcaccgag ctcttctccc cccgatcgca
     5521 catcgcctcg accgacttcg gcgccgcggc cgtcaggacg cgatggcctt cctccgtcac
     5581 gaggacgtcg tcctcgatcc gtacgccgat cccgaggaac gcggacggca ccttcttctc
     5641 gtcggggcgg atgtaga
//
LOCUS       DAOONL010000184         5622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_17258_length_5622_cov_19.213041, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000184 DAOONL010000000
VERSION     DAOONL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17258_length_5622_cov_19.213041"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..491)
                     /locus_tag="PLP50_16185"
     CDS             complement(21..491)
                     /locus_tag="PLP50_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53132.1"
                     /translation="MRRTVLALSTLLALFALAAGAFADAGERKSYTKTHPFGRDAEAK
                     VGIRQGPVTIESVRIRNWPDADDFADAEDDAGETHTMVVEFEYTNRDEARDWKCRYTV
                     KVPGKDGGLWAENDRTATLDAGKTHDTNKVFVRMKTRNYKKARSIQVKYEIWRD"
     gene            599..2413
                     /locus_tag="PLP50_16190"
     CDS             599..2413
                     /locus_tag="PLP50_16190"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPA53133.1"
                     /translation="MCGALRRRGRPAVGGLLVLLAFPLARTRAAADGPGEAERVRILE
                     QSIRPRSVVSSPLGMVVTSSPEASWAGARMLEAGGNAVDAAVAAAFALTAADPGGSGL
                     GGQTWLVLHLASGEERAILCPAPVPLRIDRRRLRAAQEGADLWGPMAAAVPTTVATLA
                     HALRTYGTKPFAEVLAPAVEAAESGYRTQSFEHPYLGDYRRRLFDSDVLYPVYLTGPA
                     DDSGFPGPAPLGTCVRLPNLARTLARLAERGPEDFYGGEIAATLAREVLAAGGFLTRG
                     DLARVPGRVLDLTPVRGSYRGLTALSVPAPGGGGVLVMALQVLDALGAERLAEAGLSR
                     GQAIVEAVRIARAETAAHRGQPEVPDGPLRSEWLSPAWAERQARRIRADRALPPSELG
                     DERPFAAGIRGTSHVSVADAEGNVVSLTQTLGRYYGAAWAAPGLGFLLNAFLEPLVSD
                     DPASAEYLKAGGAAQMPVSPLLLLRDGRPVLVAGVPGSSRIPSILLNLVVGLVDGGDG
                     IAAAAARPRMIWEDDLAGPRVMLELAPPFTERDVAALEAMGYPKVWVLRAPSRDSGVF
                     GGINGIARDEARGSWTGIVDGRRAGFAAAPARVPPPAR"
     gene            complement(2448..3353)
                     /locus_tag="PLP50_16195"
     CDS             complement(2448..3353)
                     /locus_tag="PLP50_16195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA53134.1"
                     /translation="MSSTPNLRAGFAGLGTMGLGMARNLAAKGFPLSLTNRTMEKAIR
                     LASDLAGGPAPVAALPTPAAVAAASDVVVSCVSDGPDVEEVHLGRNGTIEGARPGTVV
                     VDCSTVDPAVARRVAARLAEKGVAFLDAPVSGGQKGANEGTLSFFVGGEAEALGKARP
                     VLEAMGRRITHLGPSGAGQLGKATNQVIVAGTLAAVSEGMAFAKAAGLPMEAIHAALT
                     GGAANSWALEVLGKKMLDRDFKPAFAIKHQQKDLAIVLRAARANGVPLPAASLVHQLL
                     SSLEAKGRGEEGTQALFTLYEELSR"
     gene            3408..3998
                     /locus_tag="PLP50_16200"
     CDS             3408..3998
                     /locus_tag="PLP50_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53135.1"
                     /translation="MTRTAERTILPALAVVAFLAAAYAWLPFARALRYATVAVFTRPA
                     AWHLALVPALLAVALHELLVRGLLYGALRTRLRVGFAAPTVALVGAIVPVAARYLLFP
                     FPAVPTPLVLGQALFVEYPISLALCLLALGTGSWKSSAAALYLLWAARVLVVPTFRGA
                     PVPILEVLAALLVPLVVALVLHRPLAPHREALEGLS"
     gene            3995..4549
                     /locus_tag="PLP50_16205"
     CDS             3995..4549
                     /locus_tag="PLP50_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPA53136.1"
                     /translation="MKIEALVQLDLFSEAPPTRAELVADQASLQALYDAFAEAYRLPP
                     ARVELSSRRATGGVIQYGPPHVIRVSAHMSPADRRETLLHETAHAICHDRWGVDEGHS
                     SRFWEIAKELGVSRRSAPETERLAAVRARNARYVYRCIGCLGEWTRRTAFGRARLCAA
                     CESKGRPSRLVLVRRPKRAGRRKA"
     gene            4805..5488
                     /locus_tag="PLP50_16210"
     CDS             4805..5488
                     /locus_tag="PLP50_16210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53137.1"
                     /translation="MAAILEATRPPSAARVLDLGCGAGAHAVAFAARGFRVTGVDRSA
                     TLLEAAGRAAAEAGVEVELLKADFRTFSRPAAFDLACSLNASFAYFDDATHRRLLRLL
                     RRSLRGGGSLVLDTLGEGRAAWSGDAATHEIGGARYSVRRAFDESRGVLREEWTVERG
                     GRPETFLCEQRLFTAAELTALAEEAGFAHARASSTLDGAAPYDARSLRLVLLAQRPAS
                     SGKGPSQGV"
BASE COUNT          590 a         2331 c         1882 g          819 t
ORIGIN      
        1 ccgggtcctc cccgttcacg tcagtcccgc cagatctcgt acttgacctg gatcgacctg
       61 gccttcttgt agttccgggt cttcatccgc acgaacacct tgttcgtgtc gtgcgtcttc
      121 ccggcgtcga gcgtcgccgt ccggtcgttc tcggcccaga ggccgccgtc cttcccgggt
      181 accttcaccg tgtagcggca cttccagtcg cgtgcctcgt cgcggttcgt gtactcgaac
      241 tccacgacca tcgtgtgcgt ctcgcccgcg tcgtcctcgg cgtcggcgaa gtcgtcggcg
      301 tcgggccagt tgcggatgcg gaccgactcg atcgtgaccg ggccctggcg gatccccacc
      361 ttcgcctccg cgtcccggcc gaacgggtgt gtcttcgtgt agctcttccg ctcgccggcg
      421 tcggcgaacg cgcccgccgc gagggcgaag agggcgagga gcgtcgagag agcgagaacc
      481 gtgcgtctca tggggcctcc ggtcgtcgtg ctgggctcgc gccggattct acgggcgcgg
      541 cgcgccccgt cgagcgtctc gttgacagcg cccccgcgct gcgttctcct tcctgaagat
      601 gtgcggagcc ctccgccgcc gcggccggcc tgccgtgggc ggcctcctcg tcctcctcgc
      661 cttccccctc gctcggaccc gggccgccgc cgacggaccg ggcgaggccg agcgcgtccg
      721 gatcctcgag cagtcgatcc gtccccgctc cgtcgtctcc tcgccgctcg gcatggtcgt
      781 cacctcgtcg ccggaggcgt cgtgggccgg cgcacggatg ctggaagcgg gcggaaacgc
      841 cgtcgacgcg gccgtcgccg ccgcgttcgc cctcaccgcc gccgaccccg gcggctcggg
      901 cctcggcggg cagacgtggc tcgttctcca cctcgcctcc ggggaggagc gggcgatcct
      961 ctgcccggct cccgtgccgc tccggatcga ccgccgccgg ctacgtgccg cgcaggaggg
     1021 cgccgacctc tggggtccga tggccgccgc ggtcccgacg accgtcgcca cgctcgcgca
     1081 cgccctgcgc acctacggca cgaagccgtt cgcggaggtc ctcgcgccgg cggtcgaggc
     1141 cgccgagtcg ggctacagga cgcagtcctt cgagcatccc tatctcggcg actaccgccg
     1201 tcgcctcttc gactccgacg tcctctatcc cgtctacctg accggtcccg ccgacgactc
     1261 ggggtttccc ggccccgccc cgctcggcac gtgcgtgagg ctcccgaacc tcgcccggac
     1321 gctcgcgcgg ctcgcggagc gcgggccgga ggacttctac gggggcgaga tcgccgcgac
     1381 gctcgcgcgg gaggtcctcg cggccggcgg cttcctcacg cggggcgacc tcgcccgcgt
     1441 cccgggccgc gtcctcgacc tcacgccggt ccgcggctcc taccgcggcc tcaccgcgct
     1501 ctccgtcccg gcgccgggcg ggggaggcgt cctcgtcatg gcgctccagg tcctcgacgc
     1561 cctcggcgcg gagcgcctcg ccgaggccgg cctctcgcgc ggacaggcga tcgtcgaggc
     1621 ggtccgcatc gcgcgcgccg agacggcggc gcaccgcggg cagccagagg tcccggacgg
     1681 cccgctccgc tccgagtggc tctcgccggc ctgggccgag aggcaggccc ggcgaatccg
     1741 cgccgaccgg gcgctgccgc cgtccgagct cggcgacgag cggcccttcg ccgcgggaat
     1801 ccgagggacg tctcacgtct ccgtcgccga cgcggagggc aacgtcgtct cgctcacgca
     1861 gacgctcggc cgctactacg gggccgcctg ggcggccccc ggcctcggct tcctcctgaa
     1921 cgcctttctc gagcccctcg tctccgacga ccccgcctcg gccgagtacc tgaaggccgg
     1981 cggcgccgcc cagatgcccg tctcgcccct cctcctcctg cgcgacggac gccccgtcct
     2041 cgtcgccggg gtgcccggca gcagccggat cccctccatc ctcctcaacc tcgtcgtcgg
     2101 cctcgtcgac ggcggcgacg ggatcgctgc cgccgccgcg cgcccgcgga tgatctggga
     2161 ggacgacctc gccggcccgc gcgtgatgct cgagctcgcg ccacccttca ccgagcgcga
     2221 cgtcgcggcg ctcgaggcga tgggctaccc gaaggtctgg gtgctccgcg ccccgagccg
     2281 cgacagcggc gtcttcgggg gcatcaacgg catcgcgcgc gacgaggcgc gagggagctg
     2341 gacggggatc gtcgacggcc ggcgggccgg cttcgcggcc gctcccgccc gcgtcccgcc
     2401 gcccgcccgc tgaggagcgc gccccggcgg gcgcgctcct ctccgcgtca gcgcgacagc
     2461 tcttcgtaga gcgtgaagag cgcctgcgtc ccctcctcgc cgcgcccctt cgcctcgagc
     2521 gacgagagca gctggtggac gagcgaggcc gcgggaagcg gcacgccgtt cgcgcgagcg
     2581 gcgcggagca cgatcgcgag gtccttctgc tggtgcttga tcgcgaacgc cggcttgaag
     2641 tcgcggtcga gcatcttctt cccgagcacc tcgagcgccc acgagttcgc ggcgccgccc
     2701 gtcagcgcgg cgtggatcgc ctccatcgga agccccgccg ccttcgcgaa cgccatcccc
     2761 tccgacaccg ccgcgagcgt ccccgccacg atcacctggt tcgtcgcctt cccgagctgc
     2821 cccgcgcccg acgggccgag gtgcgtgatc cgcctcccca tcgcctcgag caccggcctc
     2881 gccttcccga gcgcctcggc ctcgccgccg acgaagaacg acagcgtccc ctcgttcgcc
     2941 cccttctgcc cgccggagac gggggcgtcg aggaaggcga ctcccttctc ggcgagccgc
     3001 gcggcgacgc gccgggccac ggccgggtcg accgtcgagc agtcgacgac gaccgtcccg
     3061 gggcgcgccc cttcgatcgt cccgttccgc ccgaggtgca cctcctccac gtcgggcccg
     3121 tcggagacgc acgagacgac gacgtcgctc gcggcggcaa cggcggccgg cgtcggcagc
     3181 gccgcgacgg gagcggggcc gccggcgagg tcggacgcga ggcggatcgc cttctccatc
     3241 gtccggttcg tcagggagag ggggaagccc ttcgcggcga ggttccgggc catgccgagg
     3301 cccatcgttc cgaggccggc gaagccggcg cgcaggttcg gtgtcgagct catccggcga
     3361 gtctctccgc cctgtcggga ggcgtcaacg ttcgctaaca tccacgcatg acgcggacgg
     3421 cggagcggac gatcctcccg gcgctcgccg tcgtcgcctt cctcgcggcg gcctacgcct
     3481 ggctcccctt cgcgcgcgcc ctccgctacg cgaccgtcgc cgtcttcacg cgccccgccg
     3541 cctggcacct cgccctcgtc ccggcgctcc tcgccgtcgc gctccacgag ctcctcgtcc
     3601 gcggcctcct ctacggcgcg ctccgcacgc gcctgcgcgt cggcttcgcg gcgccgaccg
     3661 tcgccctcgt cggcgcgatc gtcccggtgg cggcccgcta cctcctcttt ccgttcccgg
     3721 ccgtcccgac gccgctcgtc ctcggccagg ccctcttcgt cgagtacccg atctcgctcg
     3781 cgctctgcct cctcgcgctc ggcacgggca gctggaagtc gagcgccgcc gccctctacc
     3841 tcctctgggc cgcccgcgtc ctcgtcgtcc cgacgttccg cggcgccccc gtcccgatcc
     3901 tcgaggtcct cgccgccctc ctcgtccccc tggtcgtcgc cctcgtcctc caccggccgc
     3961 tcgcgcctca ccgcgaggcg ctggagggac tgtcgtgaag atcgaagcgc tcgtccagct
     4021 cgacctcttc tccgaggcgc cgcccacccg ggcggagctc gtcgccgacc aggcctcgct
     4081 ccaggcgctc tacgacgcct tcgccgaggc ctaccgcctc ccgcccgcgc gcgtcgagct
     4141 ctcctcgcgc cgcgccaccg gcggcgtcat ccagtacggc ccgcctcacg tcatccgcgt
     4201 ctcggcgcac atgtctcccg ccgaccgccg cgagaccctc ctccacgaga ccgcgcacgc
     4261 catctgccac gaccgctggg gcgtcgacga ggggcactcc tcccgcttct gggagatcgc
     4321 gaaggagctc ggcgtcagcc gccggagcgc ccccgagacc gagcgtctcg ccgccgtccg
     4381 ggcgcggaac gcccgctacg tctaccgctg catcggctgc ctcggcgagt ggacgcgccg
     4441 caccgccttc ggtcgcgccc gcctctgcgc ggcgtgcgag tcgaaagggc gcccctcgcg
     4501 cctcgtcctc gtccgccgtc cgaagcgggc gggccgccgc aaggcctgag gccgagaacg
     4561 tccttgcgcg ccggggtccg gccgcgcgat gctcgctcgg attgcccgtc ccggtctcgt
     4621 gaacgacgcc cctcgtccgc ccgcttcgcg cgctcggtct ctgccaccga ggggcgcgga
     4681 gcgcgcgcag acggggccgg cgcggcccgc gcgcgccccg ggctggagcg acgacgatcg
     4741 cctctgggac gcgctcgcgc ctgccctcgc ggcccccgcg cggcgcgccg tcgccgagac
     4801 ggaggtggcg gcgatcctcg aggcgacccg gccgccctcg gccgcgcgcg tcctcgacct
     4861 cggctgcggc gccggcgccc acgccgtcgc gttcgccgcc cgcggcttcc gggtgaccgg
     4921 cgtcgaccgg agcgccaccc tcctcgaggc cgccgggcgc gccgccgccg aggcgggcgt
     4981 cgaggtcgag ctcctgaagg ccgacttccg cacgttctcc cgccccgccg ccttcgacct
     5041 cgcgtgcagc ctgaacgcgt cgttcgccta cttcgacgac gccacgcacc gtcgcctcct
     5101 gaggctcctc cgccgctccc tccgcggcgg aggctctctc gtcctcgaca cgctcggcga
     5161 ggggcgggcc gcctggtcgg gcgacgccgc cacccacgag atcggcggcg cgcgctactc
     5221 cgtccgccgc gcgttcgacg aaagccgcgg cgtcctccgc gaggagtgga ccgtcgagcg
     5281 cggcggccgg ccggagacct tcctctgcga gcagcgcctc ttcaccgccg ccgagctgac
     5341 cgccctcgcg gaggaggccg gcttcgcgca cgcgagggcc tcctccaccc tcgacggcgc
     5401 cgccccctac gacgcccgct ccctccgcct cgtcctcctc gcgcagcgac ccgcgtcgag
     5461 cgggaaggga ccttcgcaag gtgtctaagc caatcggctt agacggcggc aaccgcgatg
     5521 ctcttcctgt cggaagcggg gattagattg cggaaggaaa tgtcgaagac cagaatccgc
     5581 agtcggctta tccccctctc cggctgtcta atgagcggtt ag
//
LOCUS       DAOONL010000185         5536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_17626_length_5536_cov_20.708447, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000185 DAOONL010000000
VERSION     DAOONL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17626_length_5536_cov_20.708447"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1759)
                     /locus_tag="PLP50_16215"
     CDS             complement(161..1759)
                     /locus_tag="PLP50_16215"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPA53138.1"
                     /translation="MSESLSRLRSLSACVPAVPLLVDVSRTCLATHARTSDPLLLEAF
                     PSAFSAMAALESGAVANPDEGRRVGHYWLRAPELAPGPDVRRAIEETVAKVKAFAADV
                     HSGKIAPESGGRFQNVLLVGIGGSALGPQLVADALGGASDPLRPFFFDNTDPDGMARE
                     LDRIAATGGIARTLVVVVSKSGGTKETRNGMLVAQAVYTAKGLDFGRHAVAVTQDGSD
                     LDKLARRQRWLERFPMWDWVGGRTSQLSAVGLLPAALQGFDVDGLLEGARACDAATRI
                     PDAGTNPAALLALAWYRETGGRGLKDMVVLPYKDRLLLLSRYLQQLVMESLGKEKDLA
                     GNTVHQGIAVYGNKGSTDQHAFVQQLREGVPNFFATFIEVLKDRRDGLPSLAVEEDVT
                     SGDYLHGFLLGTREALTEKGRGSVTITVDDVSARSLGALVALFERTVGLYAFLVGINA
                     YHQPGVEAGKKAAAAVLEAQRALLAALRADLGSYRTAETWATKAGVGAETAWKVLEHL
                     AANPDHGVRKRPGEDPFSASYGAA"
     gene            1819..2796
                     /locus_tag="PLP50_16220"
     CDS             1819..2796
                     /locus_tag="PLP50_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPA53139.1"
                     /translation="MSDSRQLVLGIDVGGTKILAGAVDEGGRVLGRGKVKSPFRGGPE
                     EMTGALVAAADAALAEAGARREDVAALGLGLPGPLDDERTTLLRAMNLGVERWPVAAA
                     LEPHFPGVPKRLENDVRAAALGEARYGAAKGERLVVAIWVGTGIGGAVLLDGDLWLGR
                     NRNAGEIGQSFIDLKKAGSFDGTLEGIGAKVGMTAFLRRRIERGEKTTVARAVLEKDG
                     RLKGSEVRKALAAGDALVERALARSARAVGVTIATLWNVFSPDLFVLGGGIAEDVGEP
                     YVEQVRAAAGRYAFFTDLAEVRVARSALGDDAGLLGAAVLAREGHRNRG"
     gene            2866..3660
                     /gene="ricT"
                     /locus_tag="PLP50_16225"
     CDS             2866..3660
                     /gene="ricT"
                     /locus_tag="PLP50_16225"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPA53140.1"
                     /translation="MSCATCSIGTLVNPGGNYVGARLADATDGKVTVVFTGEVAVRTG
                     ERIVVETEEGPEFAEVTTLTPVLAKACSQKKAKRFLRLASDSESADFAERLELQEETA
                     RWASKRIAELRLPVSLVKCVVPFDRRRITLLYSSDERANLRDLCREMSDRFRLRVESR
                     MVGVRDETKIVGGIGPCGLTLCCSTWLKGFHPVTIRMAKLQGITPNPSKLTGMCGRLK
                     CCLAYELEGGIEAARKMGSKGPDRKDAEKKAPPAPPAPPAPAPPAA"
     gene            3734..4159
                     /locus_tag="PLP50_16230"
     CDS             3734..4159
                     /locus_tag="PLP50_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPA53141.1"
                     /translation="MAAVRKPPSPASPAGARDPESELGPRKLIRPALSDLRRDMGPRG
                     LRKGAPPEQTNAESFYYLKQMQARTPVVVLLVDGEELRGWIEWYDKDAIKLNREHAPN
                     LLLQKHAIKYLYKEEEERPFRRRKVRAEPDGGSREPEGR"
     gene            4215..>5536
                     /locus_tag="PLP50_16235"
     CDS             4215..>5536
                     /locus_tag="PLP50_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPA53142.1"
                     /translation="MKTEKLTREALTAYAAENREAFEALLKDFVEIPSVSVDPTKKNA
                     IRENAKLAADVIRRFGGTSRILETDGHPIVAGAWGKNPLWPTVTVYNHLDVQPASIET
                     EPWKTEPFTFTKQGDRYFGRGTTDDKGPALTALYGIKAALDAELPVNIRVLWEFEEEI
                     GSPSFEKAVAAHADLLATDSVLVSDTIWIARGKPACPAGLRGLQGFLFTLETGETDQH
                     SGVCGGAARNPMAELMKLVNDCFDATTGRVKIPGFYDDVVPPTRTELEDFKRSGFTVA
                     GFKKDHLFRSLRTKDALDLMKRIWAQPTFEIHGMVGGYTGPGVKTVIPPSAEVKVSCR
                     LVPNQTGEKIAKLVTKFVKSKNKDVVVHVKNRLDPYQGRTEGPLAEAVKRSMKFGFKR
                     EPVFVREGGSIGAVVTMEKVLKCPVIFLGLSLPEHGYHAPNENFDWEQA"
BASE COUNT          857 a         1919 c         2020 g          740 t
ORIGIN      
        1 gcgctgacgc cgaagccggg ctgggccctt cgcggcggca tcgccgaggg gccgcagggg
       61 ccggtcttct tcaagatggt cgggccgaag aagacggtcc tggccgcgac gccctccttc
      121 gactccttcc tgaaggcgct gaagaagggc tgagcgcctc tcaggccgcg ccgtaggagg
      181 ccgagaaggg gtcctctccg ggccgcttgc gcaccccgtg gtcggggttc gcggcgaggt
      241 gctcgaggac cttccatgcg gtctcggccc cgacaccggc cttcgtcgcc caggtctcgg
      301 cggtccggta cgagccgaga tcggcccgga gcgccgcgag gagtgcccgc tgcgcttcga
      361 gaacggccgc ggcggccttt ttccccgcct cgacacccgg ctggtggtag gcgttgatcc
      421 cgacgaggaa ggcatagagg ccgaccgtcc gctcgaagag cgcgacgagc gctccgagcg
      481 agcgcgccga gacgtcgtcg accgtgatcg tcaccgagcc gcgccccttc tcggtcagcg
      541 cctcgcgcgt cccgagcagg aagccgtgga ggtagtcgcc cgaggtgacg tcctcctcga
      601 cggccagcga cgggaggccg tctctccggt ccttcaggac ctcgatgaag gtcgcgaaga
      661 agttcgggac cccctcgcgg agctgctgga cgaaggcgtg ctggtcggtc gaccccttgt
      721 tcccgtagac ggcgattccc tggtggaccg tgttccccgc gaggtccttc tccttcccga
      781 gcgactccat cacgagctgc tggaggtagc gcgacaggag gaggagccgg tccttgtacg
      841 ggaggacgac catgtccttc aggccgcggc ctcccgtctc gcggtaccag gcgagggcca
      901 ggagcgcggc cgggttcgtc ccggcgtcgg ggatccgggt cgccgcgtcg caggcgcgtg
      961 ccccctcgag caggccgtcg acatcgaagc cctggagcgc ggccgggagg aggccgacgg
     1021 ccgagagctg cgaggtccgt ccgccgaccc agtcccacat cgggaaacgc tcgagccacc
     1081 gctgccgccg ggcgagcttg tcgaggtcgc tcccgtcctg cgtgacggcg acggcgtgcc
     1141 ggccgaagtc gagccccttc gcggtgtaga cggcctgtgc gacgagcatc ccgttgcgcg
     1201 tctccttcgt tccgcccgac ttggagacga cgacgacgag cgtccgcgcg atcccgccgg
     1261 tcgcggcgat ccggtcgagc tcgcgggcca ttccgtcggg gtcggtgttg tcgaagaaga
     1321 acgggcggag ggggtccgag gcgccaccga gcgcgtcggc gacgagctgc ggcccgaggg
     1381 ccgacccgcc gatcccgacg agaaggacgt tctggaacct gccgcccgac tcgggagcga
     1441 tcttcccgga gtggacgtcg gcggcgaaag ccttcacctt cgcgaccgtc tcctcgatcg
     1501 cccgcctcac gtccggtccc ggggcgagct cgggtgcccg gagccagtag tgcccgaccc
     1561 ggcgcccctc gtcggggttc gcgacggccc ccgactcgag cgccgccatc gccgagaacg
     1621 cggacgggaa cgcctcgagc aggagggggt cgctcgttcg cgcgtgcgtg gcgaggcagg
     1681 tccgcgagac gtccacgagg agcgggacgg cgggaacgca ggcggagagg ctccggagac
     1741 gggagagcga ttcggacaag cttcacctcg ggtcgatcgg gatgcgatcc ccgtagacta
     1801 accgcaaagg agcgtcccat gtccgacagc cggcagctcg tcctcggaat cgacgtgggc
     1861 ggaacgaaga tcctcgcggg ggccgtcgac gaggggggac gcgtcctcgg ccggggaaag
     1921 gtgaagagcc cgttccgggg cggaccggag gagatgacgg gcgcgctcgt cgccgcggcc
     1981 gacgcggcgc tcgcggaggc gggcgcgcgg cgggaggacg tcgcggccct cggactcggc
     2041 ctgccgggtc cgctcgacga cgagcggacg acgctccttc gcgcgatgaa cctcggggtg
     2101 gagcggtggc cggtcgccgc cgcgctcgag ccgcacttcc ccggggtccc gaagcggctc
     2161 gagaacgacg tgcgggccgc cgcgctcggc gaggcgcgtt acggcgcggc gaagggggag
     2221 cggctcgtcg tggcgatctg ggtcgggacg gggatcggcg gcgccgtcct cctggacggc
     2281 gacctctggc tcgggcgcaa ccgcaacgcc ggcgagatcg ggcagagctt catcgacctg
     2341 aagaaggccg ggagcttcga cgggacgctc gaggggatcg gggcgaaggt cgggatgacg
     2401 gccttcctgc gccggaggat cgagcgcggg gagaagacca ccgtggcgcg cgcggtcctc
     2461 gagaaggacg gccgactcaa ggggtcggaa gtgcggaagg cgctcgccgc gggcgacgcg
     2521 ctcgtcgaga gggcgctcgc ccggtcggcc cgcgccgtcg gggtgacgat cgcgacgctc
     2581 tggaacgtct tctcgcccga cctcttcgtc ctcggcggcg ggatcgccga ggacgtcggc
     2641 gagccgtacg tcgagcaggt gcgggcggcc gcgggccgct acgccttctt caccgacctc
     2701 gccgaggtgc gggtcgcccg ctcggcgctc ggggacgacg ccgggctcct cggcgccgcg
     2761 gtcctcgcgc gcgaggggca ccggaatcgg ggctgaaggc cgcggatcgg cgcgatccgg
     2821 ccccggtttg accggacgcg gccggatcgt gacaatcaaa ggttcgtgag ctgtgccacc
     2881 tgctccatcg ggaccctcgt gaaccccggc ggcaactacg tcggggcccg cctcgccgac
     2941 gcgacggacg ggaaggtgac ggtcgtcttc accggcgagg tcgccgtccg gacgggcgag
     3001 cggatcgtcg tcgagaccga ggaggggccc gagttcgccg aagtgacgac cctgacgccc
     3061 gtcctggcga aggcctgctc ccagaagaag gcgaagcggt tcctccgcct cgcctccgac
     3121 tccgagtcgg ccgacttcgc cgagcggctc gagctccagg aagagacggc ccggtgggcc
     3181 tcgaagcgga tcgcggagct gcgtctcccg gtgagcctcg tgaagtgcgt cgtgccgttc
     3241 gaccggcgcc ggatcacgct cctctactcc tccgacgagc gggccaacct gcgcgacctc
     3301 tgccgggaga tgtccgaccg gttccgcctg cgggtcgagt cgcggatggt cggcgtgagg
     3361 gacgagacga agatcgtcgg cgggatcggg ccttgcggcc tgacactctg ctgctcgacg
     3421 tggctgaagg gcttccaccc ggtcacgatc cggatggcga agctccaggg gatcacgccg
     3481 aacccctcga agctgaccgg gatgtgcggg cgcctgaagt gctgcctcgc ctacgagctc
     3541 gaagggggga tcgaggcggc gcggaagatg gggagcaagg gtcccgaccg gaaggacgcc
     3601 gagaagaagg ccccgcccgc cccgcccgcc ccgccggctc ccgcccctcc ggccgcctga
     3661 cggcgtcccc gtcggttaga cttcgcccac ggctcgatcc ccgaaacggc ctcccgcccc
     3721 ggaaggagca tccatggctg ctgtccgcaa gccaccgtcg ccggcctcgc cggcgggcgc
     3781 acgcgacccc gagagcgagc tcgggccgcg aaagctgatt cgccccgccc tgtcggacct
     3841 ccgccgtgac atgggcccgc gcggcctgcg gaagggggcg ccccccgagc agacgaacgc
     3901 cgagtcgttc tactacctca agcagatgca ggcgcgcacg ccggtcgtcg tcctcctcgt
     3961 cgacggcgag gagctgcgcg gctggatcga gtggtacgac aaggacgcga tcaagctgaa
     4021 ccgggagcac gccccgaacc tcctcctcca gaagcacgcc atcaagtacc tctacaagga
     4081 agaggaggag cggcccttcc ggcggcggaa ggtgcgcgcc gagccggacg gcggctcccg
     4141 ggagccggaa ggccgctgac cctgctgccg cgccgcagcg cacgccgcgt cggaagcggc
     4201 gctagactcg gcccatgaag accgagaagc tcacgcgcga agccctgaca gcgtacgccg
     4261 cggagaatcg ggaggctttc gaggcccttc tgaaggactt cgtcgagatc ccgagcgtct
     4321 ccgtggaccc gacgaagaag aacgccatcc gcgagaacgc caagctcgcg gcggacgtga
     4381 tccgccgttt cggcggcacg tcccgcatcc tcgagaccga cggccacccg atcgtcgccg
     4441 gcgcctgggg gaagaacccg ctctggccga ccgtcacggt ctacaaccac ctcgacgtcc
     4501 agcccgcttc gatcgagacg gagccgtgga agacggagcc gttcacgttc acgaagcagg
     4561 gagaccgcta cttcggccgg ggcacgaccg acgacaaggg gccggcgctg acggcgctct
     4621 acggcatcaa ggccgccctc gacgccgagc tcccggtgaa catccgggtc ctctgggagt
     4681 tcgaggagga gatcggctcc ccgagcttcg agaaggcggt cgccgcccac gccgacctcc
     4741 tcgcgaccga ctccgtcctc gtctccgaca cgatctggat cgcgcgcgga aagccggcct
     4801 gcccggccgg gctccgcggc cttcaggggt tcctcttcac gctcgagacg ggcgagaccg
     4861 accagcactc gggcgtctgc ggcggcgccg cgcgcaaccc gatggccgag ctgatgaagc
     4921 tcgtgaacga ctgcttcgac gcgacgacgg gccgggtgaa gattcccggc ttctacgacg
     4981 acgtcgtccc gccgacgagg acggagctcg aggacttcaa gcggtccggc ttcacggtcg
     5041 cggggttcaa gaaggaccac ctcttccggt cgctccggac gaaggacgcc ctcgacctga
     5101 tgaagcggat ctgggcgcag ccgacgttcg agatccacgg gatggtcggc ggctacacgg
     5161 ggcccggagt gaagaccgtc atcccgccga gcgccgaggt gaaggtctcc tgccgcctcg
     5221 tcccgaacca gacgggggag aagatcgcga agctcgtcac gaagttcgtg aagtcgaaga
     5281 acaaggacgt cgtcgtccac gtcaagaacc gcctcgaccc gtaccagggg cggacggagg
     5341 gcccgctcgc cgaggcggtg aagcgctcga tgaagttcgg gttcaagcgc gagcccgtct
     5401 tcgtccgcga gggaggctcc atcggcgccg tcgtgacgat ggagaaggtc ctgaagtgcc
     5461 cggtcatctt cctcggcctc tcgctcccgg agcacggcta ccacgccccg aacgagaact
     5521 tcgactggga gcaggc
//
LOCUS       DAOONL010000186         5534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_17635_length_5534_cov_9.160431, whole genome shotgun sequence.
ACCESSION   DAOONL010000186 DAOONL010000000
VERSION     DAOONL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17635_length_5534_cov_9.160431"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..96
                     /locus_tag="PLP50_16240"
     tRNA            11..96
                     /locus_tag="PLP50_16240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Leu,seq:caa)
     gene            complement(174..866)
                     /locus_tag="PLP50_16245"
     CDS             complement(174..866)
                     /locus_tag="PLP50_16245"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPA53143.1"
                     /translation="MKIQLPGSILGPSTFADFDAAPEPSGVDLLVYVGGAISPSEYLA
                     RRETSTSALLTPLRPDGTRHSALVLPCSPALPGRPEDAWEVFLDHFHHEVLPALPLRP
                     ERMAVIGFSLGAALAVLLARAEGARVVALAAVGAVGAAEALADPGPELGPLACPVLLA
                     WNTGDPCAPHSERFVAALEAAGVAHDVTRGEGGHGFRDYVGNGRMSSAAAFGAKNLTP
                     AVRPDRPPVSGA"
     gene            1888..2280
                     /locus_tag="PLP50_16250"
     CDS             1888..2280
                     /locus_tag="PLP50_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53144.1"
                     /translation="MKQVLILVAASFTLVGCGSTGVLPVGNASALPKPEGCQVAVFDS
                     EEDVGRPFEKLCIIDAKTGSTLYSNRSVSGAMKRVNAAACQCGADAVIVKAMGKRGVS
                     AWDWGSSKVSVVAIRFTGPAVTSPAGSN"
     gene            2379..2621
                     /locus_tag="PLP50_16255"
     CDS             2379..2621
                     /locus_tag="PLP50_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53145.1"
                     /translation="MREPLYLPEGIQPRAMTEAEVRLFIDFLTAKAEQYWAHHAAARS
                     QHRDYAHGFKLEAKTYEDVHRSLLWILDGRDPMTGL"
     gene            2795..3361
                     /locus_tag="PLP50_16260"
     CDS             2795..3361
                     /locus_tag="PLP50_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53146.1"
                     /translation="MEMLAAVLVLAQSVAATPSPVPPTLTPVPTTAASPASPPSAARK
                     LSGGFGRKQKAPGKTKVVLTDEALTPSRKTGTFSVSGTRTPAKAEIPVDGSETPAKAD
                     DESTWKARVATLRVELAQAEKEYDVADRANTVIAFGEVGDEYHRMMAIRNAALTPYRI
                     KLDELRSAFAALPEECRRAPGCQPGWVR"
     gene            3548..3733
                     /locus_tag="PLP50_16265"
     CDS             3548..3733
                     /locus_tag="PLP50_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53147.1"
                     /translation="MIPHLTSLQANGVLEALAKQRTPAARSSSSAREKASKVRYHEET
                     ILPILTAGQNPMKGAGE"
     gene            3730..4290
                     /locus_tag="PLP50_16270"
     CDS             3730..4290
                     /locus_tag="PLP50_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxE family protein"
                     /protein_id="HPA53148.1"
                     /translation="MTAASLERLLKLRLAVARFGEMDGARWWNTNGQLGKLGAMALRR
                     GFPRTHRFAQARAVFAVAGHRCREVFDPPQCVTLWDLPANVEEEFEAMWERWLEQAEE
                     WKPFFEALEKPAGADLGAVLRGLDLVTDVDLEALARLRRSADGRAVQLPGVFTGADAD
                     LTLLALGFARGEPGTLAVPYMRKADA"
     gene            4287..5084
                     /locus_tag="PLP50_16275"
     CDS             4287..5084
                     /locus_tag="PLP50_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HPA53149.1"
                     /translation="MTKRGQEASRANVVSSFTIVKGTMIEETYAALAAWDFDRTKRQN
                     LDHVREVNLVGAKSQTWLRDVGKVLNRRFDPAGRDRPLALLAKAGWGLDEWKPALLWH
                     MTRDEFLFRDFLVGWLYPNWAAGAHRLRPEELHDFLREVGRRGGVTEHAWEEATLERV
                     AIGLLRMAADFGLLKGSVVREFASYHLPERSFLYLLHAFCDAEPNPRRLIESPEWKMF
                     LMESADVERELLRLHQFQKLHYDVAGSLSQLTLPCASALDYAGRMAA"
     gene            5081..>5534
                     /locus_tag="PLP50_16280"
     CDS             5081..>5534
                     /locus_tag="PLP50_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HPA53150.1"
                     /translation="MSDLEQRIKTVLEPILELPDPRTRLSAYHDMPYAIFRYDPEEEL
                     EMRRLVSLLATRLAQKGKRVSRISLAECLDEALRSQNPLEDWISAEREQGPSTLVDTI
                     HEVLSSYAPLVGLVASRMPADPDPLRDVVFLLRTGALFPVYRTFSLLEQ"
BASE COUNT          897 a         1890 c         1879 g          868 t
ORIGIN      
        1 cgcgttccgc gccggagtgg cggaacggca gacgcagggg actcaaaatc ccccgggcgc
       61 aagctcatga gggttcgagt ccctcctccg gcaccattga atccccggga cttaggcccg
      121 gaagggcctc ccgccccgct catcgagcgg gtgaggattg ccttcagcgt gcctcaggcg
      181 ccggaaaccg gagggcggtc cgggcggacg gcgggcgtca aattcttggc gccaaaggcg
      241 gccgcagagc tcatccggcc attccccacg taatccctga acccgtgccc gccctcgccg
      301 cgtgtgacgt cgtgagccac gccggccgcc tcgagcgccg ccacgaaccg ctccgagtgc
      361 ggcgcgcatg ggtcgccggt gttccaggcc aggaggaccg ggcaggcgag ggggccgagc
      421 tcaggcccgg ggtccgcgag cgcctcggcc gctccgaccg ccccgaccgc cgccagggcc
      481 acgacgcgcg ccccttccgc ccgggcgagg agaaccgcga gggccgcccc gagggagaac
      541 ccgatgacgg ccatccgttc aggtcggagc ggcagagccg gcaacacctc gtgatggaag
      601 tgatcgagaa agacctccca ggcatcctcg ggtcgccccg ggagcgccgg ggagcagggg
      661 agtacgagcg ccgagtgacg cgtcccgtcc ggccgaagag gtgtcaggag cgcgctcgtc
      721 gaggtttccc gccgggcgag gtactcactt ggggagatgg cgccgccgac gtagacgaga
      781 agatcgactc cagacggctc gggagccgca tcgaagtcgg cgaaggtgct cggtcccagg
      841 atggaacctg gcagctgaat cttcacgcgc tctgcgtccg aacggtgact cccgtcgagc
      901 tcacgacgat gggcgggcgt gtccgcggaa agtaacgggg ccggcgctgc ctccctgcgc
      961 gactgccggt aggtcggcac ttcggcgggg aagcggaggg gaagaaacgc ggcactcttc
     1021 aggcgcctac ttcgccggag gcaggccgaa cgcctttcga gcgaagtctg ccgtctctct
     1081 cgcgtcccgc cgaagtgact cgagctcgga aggactcagc atctcggaca ctggcgggga
     1141 agacggcttc ggctgcggcg acggcttcgc cttcggactg cgccgctgcc tcaagatggt
     1201 ttctgaggtt cgcctccgag agctcacggg gctccggggg cgatatgcac tcgggtcgtc
     1261 gcgatcatcg cggccgtctc gaggaacgtg gcggcgggcc cacgggcgtc gactcggagc
     1321 ctttggaggt gccggcagct cgcgtttcag gctcggatgg aaccaggaac agcccctcct
     1381 cgaccttccg gtagcggaag ggattcacgt ccaggaacgg cttcgtccag agacgggata
     1441 cctctcgaag cgcagggttc ttgctggtgc ggagtatgtc gaatcggccg atcaccccgt
     1501 gcatctccta cctcagttca cagggagatc ttcccgggcg agggcggcct tgatccgggc
     1561 gtgtcgtacc agacgctggt agaccctctc ttccccgggg gttctggctt cggtgcgcga
     1621 cgaagtggcg gacgattcga ccgaacgcgc ggggcgtgga gagtccgtcg gtgaaggggg
     1681 cttcttctcg gtcatgtcct ccggaatgtg gggcgcgtgg tccgcatcgt caaggtcacc
     1741 gcgccgtcgc ccggctatga acatcgctcc gcggcgatcc agagcagatg cgagcgccag
     1801 ctcccgtctc acgtatgacg agcttccttc acgaacatgg acaccatctg tccgtgtggc
     1861 ggcgtacggt ctccggcgga ggagtatatg aagcaggtcc tcatcctcgt tgcagcatcc
     1921 ttcacgctcg tcggatgcgg ctccaccggc gtccttcccg tcggcaacgc cagtgccttg
     1981 ccgaagcccg aggggtgcca ggtcgcggtc ttcgactccg aggaggacgt tggccggccc
     2041 ttcgagaagc tctgcatcat cgacgcgaag accggctcga cgctctacag caacaggtcg
     2101 gtttccggag cgatgaagag ggtgaacgcg gccgcctgcc agtgcggagc cgacgccgtc
     2161 atcgtgaagg ccatggggaa gcggggcgtc tccgcctggg actggggatc ttcgaaggtc
     2221 tcggtcgtcg caattcggtt caccggcccg gccgtgacct cgccggctgg ttcgaactga
     2281 tcgctggggc ggggccctgt cgccccaacg ccgggatttc gagatcatcc ggactggatc
     2341 gacgattcga ggcgacgaac ggaaactgga gggactcggt gagagagcct ttgtacctgc
     2401 cggagggaat ccagccgcga gccatgacgg aggccgaggt ccggctcttc atcgacttcc
     2461 tgacggccaa ggcggagcag tactgggcgc accacgctgc ggcgaggagc cagcatcggg
     2521 actatgccca cggtttcaag ctcgaggcga agacctacga ggacgttcac cgatcgctcc
     2581 tctggatact ggacggtcgg gacccgatga cggggctgtg aacggagggg gactcgcgtg
     2641 gaaggacgtt cgcgccgcct ctcgcgctcc tggcgggagg taggcggaag gcggcccgaa
     2701 gccactcttc ggtagcgagg ccgtgcgcgc atgctccgcc cccgggacgc catttgtccg
     2761 tgtccctcct tcagctttgt cggcggagga gttcatggaa atgctcgcgg ccgtcctcgt
     2821 cctcgcccag tccgtcgccg ccacgccgag tcctgtcccg cccacgctta cgccagttcc
     2881 gaccacagcg gcgtccccgg cttcgccgcc ctcggccgcc cggaagctct ccggcggctt
     2941 cggccggaag cagaaggcgc cgggcaagac gaaggtcgtc ctcaccgacg aggcgctcac
     3001 gccgtcgcgg aagaccggga ccttcagcgt ctcgggcacg cggactccgg cgaaggccga
     3061 gatcccggta gacggatcgg agaccccggc aaaggcggac gacgagtcca cctggaaggc
     3121 tcgggtcgcg acactccgcg ttgagctggc gcaggccgag aaggaatacg acgtcgccga
     3181 ccgggccaac accgtcatcg cattcgggga ggtgggggac gaataccaca ggatgatggc
     3241 gatccgaaat gcagccctta cgccctaccg gatcaagctc gatgagctcc ggtcggcgtt
     3301 tgcggcgctt cccgaggagt gccggagagc accgggatgt cagcccgggt gggtgcggta
     3361 gcggattccg cggccaggtc acacacgtcg actgtcccgg tccgctcctc ccgcccggcg
     3421 actgcgagcg acagggaccg ggctgggggg aatgacgacc cgaggaggag gggagcatga
     3481 ctggtcacat cgtcggtgag cgtctcgtcg gcgacatgtc gtttcttctg ctgacgatcc
     3541 cgacgaagtg atcccccacc tcacttcgct ccaagccaac ggcgttctcg aggccctcgc
     3601 gaagcagagg acgccggcgg cgcgttcgtc ctcgtcggcg cgggagaagg cgtcgaaggt
     3661 tcgctaccac gaagagacga ttctgccgat actgacagcg gggcagaatc ccatgaaagg
     3721 agccggcgaa tgacggctgc gagcctcgaa cgcctcctca agctccgact cgccgtcgcc
     3781 cgcttcggcg agatggacgg agcccgctgg tggaacacga acgggcaact cgggaagctc
     3841 ggtgcgatgg cgcttcggcg cggcttcccg cggacgcacc gcttcgctca ggcccgggcg
     3901 gtcttcgcgg tcgccgggca ccgctgccgg gaggtcttcg atccgcctca atgcgtcacg
     3961 ctgtgggacc tcccggcgaa cgtcgaggag gagttcgagg cgatgtggga gcgctggctg
     4021 gagcaggcgg aggagtggaa gccgttcttc gaggccctcg agaagccggc gggcgctgac
     4081 ctcggagccg tccttcgggg cctcgacctc gtgaccgacg tggacctcga ggcgctcgcg
     4141 cgccttcgcc ggagcgccga cggaagggcc gtccaactcc ctggagtctt cacgggtgcc
     4201 gatgccgacc tcaccctcct cgccctcggc ttcgcccgag gggaaccggg aaccctcgcc
     4261 gtcccctaca tgcggaaggc ggacgcttga cgaagcgagg ccaagaggcg tcccgcgcca
     4321 acgtcgtctc ctccttcacg atcgtgaagg gaacgatgat cgaagagacc tacgcggccc
     4381 tcgccgcctg ggacttcgac cggaccaaac gccagaacct cgaccacgtc cgggaggtca
     4441 acctcgtcgg cgcgaagagc cagacctggc tccgagacgt cgggaaggtc ctcaaccgcc
     4501 gcttcgaccc tgccggccgt gaccgacctc tcgccctcct cgcgaaggcg ggctggggcc
     4561 tcgacgagtg gaagccggcc ctcctctggc acatgacccg cgacgagttc ctcttccggg
     4621 acttcctcgt cggctggctc tacccgaact gggccgcggg agcccatcgc ctccgtcccg
     4681 aggagcttca cgacttcctc cgggaggtcg gcaggcgggg tggcgtcacg gagcacgcct
     4741 gggaagaggc cacactcgag cgggtcgcca tcggcctcct gcgcatggcc gccgacttcg
     4801 gcctgctgaa gggctcggtc gtccgggagt tcgcctcgta ccaccttccg gagcggagct
     4861 tcctctacct ccttcacgct ttttgtgacg ccgagcccaa cccccgccgc ctcatcgagt
     4921 ccccggagtg gaagatgttc ctcatggagt ccgccgacgt ggagcgggag ctcctccggc
     4981 tgcaccagtt ccagaagctg cactacgacg tcgccgggag cctctcgcag ctgacgcttc
     5041 cgtgtgcgag cgccctcgac tacgccggga ggatggccgc atgagcgacc tcgaacagcg
     5101 catcaagacc gtcctcgagc cgatcctgga gctcccggac ccgcggacgc gcctcagcgc
     5161 ctaccacgac atgccgtacg ccatcttccg gtacgacccg gaagaggagc tcgagatgcg
     5221 ccgcctggtg agcctcctgg cgacccgtct cgcccagaag gggaagcgcg tctcgcggat
     5281 ctccctcgct gaatgcctcg acgaggccct tcgctcgcag aaccccctcg aagactggat
     5341 cagtgccgag cgcgagcagg ggccttccac gctcgtcgac acgatccacg aggtcctttc
     5401 ctcgtacgcc ccgctcgtcg gcctcgtcgc ctcgcggatg ccggcggatc ccgacccgct
     5461 ccgcgacgtc gtcttcctcc tccgcacggg cgccctcttt cccgtctacc gcacgttctc
     5521 gctcctcgag cagc
//
LOCUS       DAOONL010000187         5516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_17724_length_5516_cov_8.343527, whole genome shotgun sequence.
ACCESSION   DAOONL010000187 DAOONL010000000
VERSION     DAOONL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17724_length_5516_cov_8.343527"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..713)
                     /locus_tag="PLP50_16285"
     CDS             complement(138..713)
                     /locus_tag="PLP50_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53151.1"
                     /translation="MTCSDFDLDFRPRSLVLWVTASQRRAHEETVRAVESGDFPTCAP
                     LEAARRRPARRCPNGRYPLASFAAEPSANLYPCRGFLMARPIEGRRFEIEDTRNTMKG
                     VESFGKTLAPRIPSMRDVIAMIDNSLNLGWEEFGPVLSTWQETVRQRYLKWPGDWSFW
                     VRSLNYPDLEAWYRRQWKELSLQEQVRPAVT"
     gene            complement(710..4222)
                     /locus_tag="PLP50_16290"
     CDS             complement(710..4222)
                     /locus_tag="PLP50_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA53152.1"
                     /translation="MRIDRLELAAYGPFTNVSIEFDREIGLHVVFGPNEAGKSSALRA
                     VSDLLFGVPTQTTDNFLHAYDALRIRATITARDGRTLDFVRRKGRSGSDLLDASRRAL
                     ARDVLVPFLGSAERDFFERSFGLDPRRLAAGAMDLLQSGGDVGRAVLQATSGLSGLSA
                     RLDAIEAAAGELFKERGKTQPINAALSRLKELEREVRSDSLSAETWRSLDEELAHVRR
                     EAMRLADRRTANDVEQQRLSRIRQALPILARRTLLMAAVERLAGVPLLAAEFDATAQA
                     AREELLSAAAAEAGLASDLTSLEEEVSKYPAVGPILLRGTEIEALHQECGTMREFRKD
                     LPKRDVELREATAQATRLAAEIVPGLGLDALLDEVPRRPVRRALRASAGRARGLRDDL
                     AREERELATARESIRAAEAKLASLGPAVDPAPLARALRDAERSGDPVESLGKAEQAEK
                     RARKALDEALASLPGWAPRGADPLAALPVPGDALVDSFQKRFSDLETAERDLENERRS
                     LASQRSGRESERRNLAAGGTLPTRAAVGAARETRTKGWTLVRRVYVEREPGAEDEARL
                     FDPERPLVEAFERSVETADGAADRLVDAADVAAKDAELSRQLREIDEALAGCGRRAEE
                     LAVRRAAAEHEWRAAWPAEGVAPGAPASMKEWLRARSRTLERLDAFRGATAEVEAASS
                     ALERARVSLVSAVALVRPEGPPPPAAYPEAETFVEELRRALEKTSAERGKLEATIEAA
                     TGKAGAREIAVAQARTALEEWKTSWAEEVRPLRRSPTVSTEEVEAVLDACDEFESVAG
                     KVPGLRDRVKKMQDRLEEFAASVGTLCADVAPDLQRTDAVVAAGTLFERLGAERAAEG
                     KRVLARENLVKIREKLAKESKRSETARRSVEAICREAGCAGESGLPARLAEIAEKRRA
                     VKDLGEAESDLLAVGAGRSPSDLATECADVDGDALPALLEKARDERKALDEEIGRLHK
                     REGELGQSLEIANGGDVAATRGQEAASVRARIEEDARRWLRLRASAFLLRRAIDGWRK
                     ENQDPLLVEATQLFSSLTKGRFVSLLAEVDDHGDPVITAGRAEGRHVPMAGLSDGTRD
                     QLFLALRLASVRRYAKDAEPLPFIADDLLVSFDDSRAAAALETLSEFGKEVQVVLFTH
                     HRHIVDLAKAHLAASAFRVHELLS"
     gene            complement(4219..5478)
                     /locus_tag="PLP50_16295"
     CDS             complement(4219..5478)
                     /locus_tag="PLP50_16295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPA53153.1"
                     /translation="MRLVHAADIHLDSPLRGLDRYDGAPSEDLRIATRRAFVYLIDYC
                     LEESVDGLLLAGDIYDGDWQDFNTGLFFVKQLARLRDGGIRVWAVLGNHDAASVITRQ
                     LRLPDNVTLLSYKSPVTSVDEQLGLAVHGQSFARKDVGEDLAAAYPRALPGLLNVGLL
                     HTALAGRPDHAPYAPTTVDRLTSKGYGYWALGHVHRAEVVSRDPWIVFPGNLQGRSIR
                     ETGEKGFVVISSEGTEVTGVETVASDVARWHDLSVDVSSARNADDALTLVLRALDATA
                     GASKGRLVAARVRLTGRGPVHTVLVRDLAATEANVRAAAADASGDVWVEKVVVQTLPE
                     VDLEKVRERKDALGHVARAVHGAAGDPEALKALAEELRPLLEKLPSGLGEDGRLFDAS
                     EPARLEAFLSEVEATLVPMLVEVEERP"
BASE COUNT          758 a         1948 c         1926 g          884 t
ORIGIN      
        1 ggcaggcatt cctctcccac cgggagaagg ggacgctcag cctctcgacc cagatgccgt
       61 tttcccgctc ctcatccacc tcttctgaga agaggcatgc aacccccgca ccccatctga
      121 tccgatgggg tgctttgtca tgtgacggcg gggcggacct gttcctggag gctcagttct
      181 ttccactgtc gtcgatacca cgcctcgagg tccgggtagt tcagcgagcg gacccagaac
      241 gaccagtccc cgggccattt caggtagcgc tggcgtaccg tctcctgcca cgtggagagg
      301 acggggccaa actcctccca gccgagattc agggagttgt cgatcatggc gatcacatcc
      361 cgcatcgacg ggattctggg agccagtgtc tttccgaagg actcgacccc cttcatcgtg
      421 ttcctggtgt cctcgatctc gaaccgtcgc ccctcgatcg ggcgcgccat cagaaacccg
      481 cgacacgggt agaggttcgc gctcggctcg gccgcgaacg acgcgagtgg gtagcggccg
      541 tttgggcagc ggcgcgcggg tcgccggcga gcagcttcga gaggggcgca ggtggggaag
      601 tccccgctct cgacggcgcg gaccgtctcc tcatgggctc ggcgctggct ggcggtcacc
      661 catagcacga gagatctggg ccggaagtcg aggtcgaagt cggagcaggt caagacagaa
      721 gctcgtgcac ccggaacgcg ctcgccgcga ggtgggcctt ggcaaggtcg acgatgtgac
      781 gatggtgggt aaagagaacg acctgcacct ctttcccgaa ctccgatagc gtctccagcg
      841 ccgccgccgc tctcgagtcg tcgaaggaga caaggaggtc gtcggcgatg aaggggagcg
      901 gctcggcgtc cttcgcatag cgccgtacgg aggcgagccg gagggccagg aagagctggt
      961 cgcgcgtgcc gtcgctcagg ccggccatcg gcacgtgccg cccctcggct ctcccagcgg
     1021 tgatcaccgg gtccccgtgg tcgtcgacct ccgcgaggag cgagacgaag cgtcccttcg
     1081 tcagcgagga gaagagctgc gtggcctcga cgaggagggg gtcctggttc tccttcctcc
     1141 agccgtcgat cgcgcgccgg aggagaaatg cggaggcccg gagccgcagc caacgccgtg
     1201 cgtcctcctc gatccgggca cggacgctcg cggcctcctg cccgcgcgtt gcggcgacgt
     1261 cgccgccgtt ggcgatctcg aggctctgcc ccagctcacc ctcgcgcttg tggagccggc
     1321 cgatctcctc gtcgagggcc ttccgctcgt ccctggcctt ctcgaggagc gccgggaggg
     1381 cgtctccgtc cacatcggca cactcggttg cgagatcgga gggagagcgg cccgcgccca
     1441 cggcgagcag gtcactctcc gcctccccga ggtccttcac ggcacggcgc ttctcggcga
     1501 tctccgcgag gcgggccgga aggccggact ctcccgcgca gccggcctcg cggcagatcg
     1561 cctcgacgct ccgccgtgcg gtctcgctcc gcttgctctc tttcgcgagc ttctcgcgga
     1621 tcttcaccag gttctcccgg gccagcaccc tcttcccctc ggccgcgcgc tcggcgccta
     1681 ggcgctcgaa gagcgtcccg gcggctacga cggcgtcggt ccgctgaagg tccggggcga
     1741 cgtcggcgca gagcgtgccg accgaggcgg caaattcctc gaggcggtcc tgcatcttct
     1801 tgacgcggtc ccgcaggccg ggcaccttgc ccgcaactga ctcgaactcg tcgcaggcgt
     1861 cgaggacggc ctccacctcc tcggtcgaga cggtggggga acgccggagg ggccggacct
     1921 cctcggccca cgaggtcttc cactcctcga gcgccgtgcg agcctgagcg acggcgatct
     1981 cgcgggcgcc tgccttcccg gttgccgcct cgatcgtcgc ctcgagcttt cccctctcgg
     2041 cggaggtctt ctcgagcgct cgccgcagtt cctcgacgaa cgtctcagcc tcgggatacg
     2101 cggcgggcgg cgggggcccc tcgggacgga cgagcgcgac ggccgaaacg agagacacgc
     2161 gagccctctc gagggccgaa ctcgcggcct cgacctcggc cgtggcgccg cggaacgcgt
     2221 cgagccgctc gagtgtccgg cttcttgcac ggagccactc cttcatcgat gccggggcgc
     2281 cgggcgcgac gccttcggcc ggccaggcgg ctctccactc gtgctcggcg gcggcgcgcc
     2341 gtacggcgag ctcctcggcg cgccgcccgc atccagcgag ggcttcgtcg atctcccgaa
     2401 gctgccggga gagctccgca tccttcgccg ccacgtcggc ggcgtccacg agccggtcgg
     2461 cggcgccgtc cgccgtttcc acggaacgct cgaaggcttc gacgagcggg cgctccgggt
     2521 cgaagaggcg cgcctcgtcc tcggcgcctg gctctctctc gacgtagacg cgacggacga
     2581 gcgtccagcc cttcgtccgc gtctcgcggg ccgcaccgac ggccgcccgg gtcggcagcg
     2641 tgccgccggc ggcgaggttc cgcctctcgg actcacggcc ggatcgctgg gaggcgagcg
     2701 agcgccgctc gttctcgaga tcgcgttcgg ccgtctcgag atcggagaag cgcttctgaa
     2761 acgagtcgac caaggcgtct ccggggacgg ggagagccgc gagcgggtcc gccccgcgcg
     2821 gcgcccagcc tggaagcgac gcgagggcct cgtcgagcgc cttccgcgcc ctcttctccg
     2881 cctgctccgc cttcccgagg ctctccaccg ggtcgccgct gcgctccgcg tcccgtaggg
     2941 ctctcgccag gggtgccggg tcgacggccg gcccgagcga cgcgagcttc gcctcggctg
     3001 cgcggatcga ctcacgtgcg gtcgcgagct cccgctcttc gcgggcgagg tcgtcgcgca
     3061 ggccgcgagc cctgccggcg cttgcacgga gagccctgcg gacgggccga cgcgggacct
     3121 cgtcgaggag cgcgtccagt ccgaggccgg gcacgatctc cgcggcgagc cgtgtcgcct
     3181 gcgcggtcgc ctcccgcagc tccacgtcgc gcttcgggag gtccttgcgg aactcgcgca
     3241 tggtgccgca ctcctggtgg agcgcctcga tctccgtccc ccgaaggagg atcgggccaa
     3301 cggccgggta cttcgagacc tcttcctcga ggctcgtcag atcgctcgcg aggcccgcct
     3361 cggcagccgc ggcgctcagc agctcctcgc gggcggcctg cgcagttgcg tcgaattccg
     3421 cggcgaggag cggaactccg gcgagccgtt cgacggccgc catcagcagg gtcctgcgcg
     3481 cgaggatcgg gagcgcctgc cggatgcgcg agagccgctg ctgctcgacg tcgttcgctg
     3541 ttctccgatc ggcgagcctc atagcttcgc gtcggacgtg cgcgagctct tcgtcgaggc
     3601 tcctccaggt ctccgcgctc aggctgtcgg accttacctc tcgttccagc tccttcagtc
     3661 gcgatagggc ggcattgatc ggttgcgtct tccccctctc cttgaacagt tcgcccgcag
     3721 ccgcctcgat cgcgtcgagg cgcgccgaga gccccgagag gccggacgtc gcctgcagca
     3781 cggcccggcc gacgtcaccc ccgctttgaa ggaggtccat cgcccccgcg gcgagccgcc
     3841 tggggtcgag gccgaagctc cgctcgaaga agtcccgctc cgcgctgccg aggaacggga
     3901 cgaggacgtc gcgcgccaga gcgcgtctcg acgcgtcgag gaggtcgctc ccgctccttc
     3961 ccttgcggcg gacgaaatcg agcgtccggc cgtcgcgcgc ggtgatcgtc gcccggatcc
     4021 tgagggcgtc gtacgcgtgc aggaagttgt cggtcgtctg cgtggggacg ccgaagagga
     4081 ggtcgctgac ggctcgaagg gccgaggact tcccggcctc gttcggcccg aaaacgacgt
     4141 gaaggccgat ctcccggtcg aactcgatcg agacgttcgt gaaggggccg taggcggcca
     4201 gttcgaggcg gtcgattctc acgggcgctc ctccacctcg acgagcattg ggacgagcgt
     4261 tgcctctact tcggagagaa aggcctccag ccttgccggc tctgaggcgt cgaacagtct
     4321 tccgtcttcc ccgaggccgg acggcagctt ctcgagcagg ggacgcagct cctcggcgag
     4381 cgctttcaga gcctccgggt caccggcagc gccgtggacg gcgcgggcga cgtgcccgag
     4441 ggcgtctttg cgctcgcgga ccttctcgag gtcgacctct gggagggtct ggacgacgac
     4501 cttctcgacc cagacgtccc cgctcgcgtc cgcggcggcg gcccggacgt tggcctcggt
     4561 tgccgcgagg tcgcgcacaa gaacggtgtg cactggtccc cgcccggtca gcctgacgcg
     4621 cgcggcgaca agccgcccct tgctggcgcc ggcagtcgcg tcaagagcgc gcagcacgag
     4681 cgtcagggcg tcatcggcgt tccgggccga cgagacgtct acggaaaggt cgtgccaacg
     4741 cgccacgtcg gaagcgaccg tctcgacccc cgtcacctcc gtcccttccg aggagatgac
     4801 aacgaacccc ttttcgcccg tttcgcggat gctgcggccc tggaggttgc cggggaagac
     4861 gatccagggg tcccgggaga cgacttcggc ccggtggacg tgcccgagcg cccagtagcc
     4921 gtatcccttc gaggtgaggc ggtcgactgt cgtgggggcg tagggagcgt ggtcgggccg
     4981 cccggcgagc gccgtgtgga gcaggccgac gttcaggagg cccggaaggg ctcgcgggta
     5041 ggccgcggca aggtcttcgc cgacgtcctt cctggcgaag ctctggccgt gtacggcaag
     5101 gccgagctgc tcgtcgacgg aggtcaccgg ggacttgtag gagaggaggg tgacgttgtc
     5161 gggcagccga agctggcgcg tgatgacgct ggcggcatcg tggttgccga gaacggccca
     5221 gacccgtatg cccccgtcgc ggagccgcgc gagctgcttt acgaagaaga ggccggtatt
     5281 gaagtcctgc cagtcgccat cgtagatgtc gccggcgagg agtagcccgt ccacgctctc
     5341 ctcgagacag tagtcgatga ggtagacgaa cgctcgccga gtcgcgatgc ggagatcctc
     5401 actgggcgct ccgtcgtacc ggtcgagccc ccggagcggg ctgtcgaggt ggatgtccgc
     5461 cgcgtggacc agcctcatgt ttcgtctcct tcgtggttag aacccgtgac gaaccc
//
LOCUS       DAOONL010000188         5352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_18439_length_5352_cov_23.374363, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000188 DAOONL010000000
VERSION     DAOONL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18439_length_5352_cov_23.374363"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1886)
                     /locus_tag="PLP50_16300"
     CDS             complement(84..1886)
                     /locus_tag="PLP50_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPA53154.1"
                     /translation="MTNPVHLVINGRDVRAESDWSVLEAAKHNGIKIPHFCYLEGVHQ
                     IGSCRICVVEVEGMRSLQASCTVPVRDGMVVKTNTARVKKAREVLYELMLSDHPRNCL
                     SCYRNQNCEFQKLGEEIKIPDCRFEGERSKTFVDDSSPSVYRDTSKCILCRRCVTVCS
                     EIQGVGVLGVNNRGFGTTIGPAADLPLGTANCANCGQCVMVCPVGALLTRADMTPVWN
                     ALFDPKIRTVVQTAPAVRVALGEMFGMEPGTIVTGKMAHALRLLGFDDVFDTNFAADL
                     TILEEGTEFLHRALAAVRGQDVVLPMITSCSPGWIKYVEHEFPDQLAHLSSCKSPHMM
                     LGALAKTYWAKKQGVDPKDMYVVSVMPCTAKKFEITRPEMFMDGRPTVDAVLTTRELA
                     RMIKEAGIDFNSLEEKSFDDPLGFSTGAADIFGTTGGVMEAALRTVYEVVTGREVPFP
                     ALEVTPVRGLSQVKEATFKLEDVKPEYAELEGFTVKVAVTSGLKGAKRLMKEVAAGTA
                     PWHFIEVMGCPGGCIAGGGQPRPTTRTIREARMRAIYAEDAGKPRRKSHENPSLMALY
                     DEYLGEPLGHLSHELLHTHYTPRGRFNELIVKGS"
     gene            complement(1897..>2535)
                     /locus_tag="PLP50_16305"
     CDS             complement(1897..>2535)
                     /locus_tag="PLP50_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA53155.1"
                     /translation="QVEDMPADPREIREAREEGIEILPLVAPVEFLGNGRVEGVKCVR
                     MELKGFDDAGRRKPKTVAGSEFVVPADMVIPAISQHSDLPFIDRDEVQLTKWGTLVVD
                     KETMMTRKKGVFSGGDVVRGPDVAIQAIADGKRAARAIDRYLGGTGVLNKGEEIPIPR
                     PTDETDVAEHERFPMRFLEPDSRRKGFQEVAAGFHKLNAIAEAMRCLRCDRR"
     assembly_gap    2536..2635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2636..4785)
                     /locus_tag="PLP50_16310"
     CDS             complement(<2636..4785)
                     /locus_tag="PLP50_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPA53156.1"
                     /translation="MTPEPNLTRPASKSDLDAIAARWHAEQQGKKHQLLVCAGAGCVS
                     SGCHAVKHALLAALKDAGLENETAVHETGCLGSCHLGPTLVVQPDGVLYTELKPENMK
                     GIVRQHLVHREVVEQHCWVDPETKQPVPLLSDIEFFKRQVKIATARCGSMPYASIEAY
                     IASDGYQGLWKALSTMTGEQVIQEMRASGLRGRGGGGFPVGAKWQAGFKAKGNEKIVV
                     CNADEGDPGAFMDRSLLEGDPHSVLEGMILAGYAIGASQGYVYVRAEYPIAVERLEAA
                     IRQAREWGFLGPSVMGSSFAFDVEIRIGAGAFVCGEETALMHSVEGKRGEPRQKPPFP
                     FERGIFGKPTIINNVETLANVATIVKNGGAWYASLGTPKSTGTKVFALAGKVENTGIV
                     EVPMGITLGEVVHDIAGGIPKGKRFKAAQTGGPSGGCLTREHLNTPIDYDSLVHLGTI
                     MGSGGLIVMDEDSCMVDVARFFMDFVQDESCGKCVPCRIGTKRMLEILTRITKGQGKE
                     GDIELLEELAANCKETSTCGLGQTAGNPVLSTLRLFREEYEEHIRDKYCRAGVCSDLF
                     RSPCQNACPAGVDVPGYVALIAAGRPRDAYHLVRRENPFPAICGRVCTHPCESKCRRA
                     QLDEPVAICDLKRYAADYVFANDEPHRDLVFPKKAESVGVVGAGPSGLTCAWYLARLG
                     YGVTVYESLPVAGGMLASGIPEYRLPKEVLAKEIR"
     gene            complement(4800..5324)
                     /locus_tag="PLP50_16315"
     CDS             complement(4800..5324)
                     /locus_tag="PLP50_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPA53157.1"
                     /translation="MQVQVSSPPPSPATACACGEATEEEKLARIAEVVEAFRDKPGSL
                     IQILHLAQGIYGYLPIHVQRLVAEKLDIPVSEVWGVTTFYSFFSTKPRGEYSIRVCLG
                     TACYVRGGVEIVKRLSQTLGVGVGETTPDGKFTLEVMRCIGACGLAPALLVNDKVVRQ
                     VNPDKLNRVLALCE"
BASE COUNT          736 a         1726 c         1920 g          870 t
ORIGIN      
        1 ggaaagggaa cggcccggcg cgaggccggg ccgttcgggg ttcggggccg caggagcggc
       61 ggcgcggggg gggcgccgtt cgctcagctc cccttcacga tcagttcgtt gaagcggccg
      121 cgcggggtgt agtgcgtgtg gagcagctcg tgcgaaaggt gcccgagcgg ctcgccgagg
      181 tactcgtcgt agagcgccat gagggacggg ttctcgtgcg acttgcggcg cggcttgccg
      241 gcgtcctcgg cgtagatggc gcgcatccgc gcctcgcgga tcgtccgggt cgtcgggcga
      301 ggctgtccgc cgcccgcgat gcagccgccg gggcagccca tcacctcgat gaagtgccac
      361 ggggccgtcc ccgcggcgac ctccttcatg aggcgctttg cccccttcag gccgctcgtg
      421 acggcgacct tcacggtgaa gccctcgagc tcggcgtact cgggcttgac gtcctcgagc
      481 ttgaaggtgg cctccttgac ctgcgagagg ccgcggaccg gggtgacctc gagagccggg
      541 aacgggacct cacgcccggt gacgacctcg tagaccgtcc ggagggccgc ctccatgacg
      601 ccgcccgtcg tcccgaagat gtccgcggcg ccggtcgaga agccgagcgg gtcgtcgaac
      661 gacttctcct cgagggaatt gaagtcgatc cccgcctcct tgatcatccg cgcgagctcg
      721 cgggtcgtca ggacggcgtc gacggtcggg cgcccgtcca tgaacatttc cggccgcgtg
      781 atctcgaact tcttcgcggt gcagggcatg acggagacga cgtacatgtc cttcgggtcg
      841 acgccctgct tcttcgccca gtaggtcttc gcgagggcgc cgagcatcat gtgcggcgac
      901 ttgcacgagg agaggtgggc gagctggtcg gggaactcgt gctcgacgta tttgatccac
      961 cccggcgagc aggaggtgat catcgggagg acgacatcct gtccgcgcac cgcggcgagc
     1021 gcccggtgga ggaattccgt cccctcctcg aggatcgtca ggtcggcggc gaaattcgtg
     1081 tcgaagacgt cgtcgaagcc gaggagccgg agggcgtggg ccatcttccc ggtcacgatc
     1141 gtgccgggct ccatcccgaa catctcgccg agggcgacgc ggacggcggg ggccgtctgg
     1201 acgaccgtgc ggatcttcgg gtcgaagaga gcgttccaga cgggtgtcat gtcggcgcgg
     1261 gtcaggagcg ccccgacggg gcagaccatg acgcactggc cgcagttcgc gcagttcgcc
     1321 gtgccgagcg ggaggtcggc ggcggggccg atcgtcgtcc cgaagccgcg gttgttgacg
     1381 ccgaggacgc cgaccccctg gatctcgctg cagacggtga cgcagcgacg gcagaggatg
     1441 cacttgctgg tgtcgcggta gacggagggg ctcgagtcgt cgacgaaggt cttcgaccgc
     1501 tccccctcga agcggcagtc ggggatcttg atctcctcgc ccagcttctg gaactcgcag
     1561 ttctggttcc ggtagcagga gaggcagttg cgggggtggt cggagagcat cagctcgtag
     1621 aggacctcgc gcgccttctt cacccgcgcg gtgttcgtct tcacgaccat gccgtcgcgg
     1681 acggggacgg tgcaggaggc ctggaggctc cgcatcccct cgacctcgac gacgcagatc
     1741 cggcaggagc cgatctggtg gacgccctcc aggtagcaga agtgggggat cttgatgccg
     1801 ttgtgcttgg cggcctcgag gacggaccag tcgctctcgg ccctcacgtc gcggccgttg
     1861 atgacgaggt gaacggggtt cgtcatggct ctcttcctac cgccggtcgc agcggaggca
     1921 gcgcatggcc tcggcgatgg cgttcagctt gtggaacccc gcggccacct cctggaaccc
     1981 cttccttcgg gaatcgggct cgaggaagcg catcgggaac ctctcgtgct cggcgacgtc
     2041 ggtctcgtcg gtggggcgcg ggatcgggat ctcctccccc ttgttgagga cgccggtccc
     2101 tccgaggtag cggtcgatgg cgcgcgcggc gcgcttgccg tcggcgatcg cctggatcgc
     2161 gacgtcgggc ccgcggacga cgtccccgcc cgagaagacg ccctttttgc gggtcatcat
     2221 cgtctccttg tcgacgacga gcgtccccca cttcgtcagc tggacctcgt cccggtcgat
     2281 gaacgggagg tcggagtgct ggctgatggc cgggatgacc atgtcggccg ggacgacgaa
     2341 ctcggagccg gcgacggtct tcggcttccg cctcccggcg tcgtcgaagc ccttcagctc
     2401 catcctcacg cacttgacgc cctcgacccg gccgttcccg aggaactcga cgggcgcgac
     2461 gagcgggagg atctcgatcc cctcctcgcg cgcctcgcgg atctcgcgcg ggtcggccgg
     2521 catgtcctcg acctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggat
     2641 ctccttcgcg aggacctcct tcgggaggcg gtactcgggg atgccggagg cgagcatgcc
     2701 gccggcgacc gggagcgact cgtagacggt gacgccgtag ccgaggcgcg cgaggtacca
     2761 ggcgcaggtg aggcccgagg ggccggcgcc gacgacgccg accgactcgg ccttcttcgg
     2821 gaagacgagg tcgcggtgcg gctcgtcgtt cgcgaagacg tagtcggcgg cgtagcgctt
     2881 caggtcgcag atggcgaccg gctcgtcgag ctgcgcgcgg cggcacttcg actcgcacgg
     2941 gtgggtgcag acgcggccgc agatcgccgg gaaggggttc tcgcggcgga cgaggtggta
     3001 ggcgtcgcgc gggcggcccg cggcgatgag ggccacgtac ccggggacgt cgacgcccgc
     3061 tgggcaggcg ttctggcagg gcgacctgaa gaggtcggag cagacgccgg cgcggcagta
     3121 cttgtcgcgg atgtgctcct cgtactcctc gcggaagagg cggagcgtgg agaggacggg
     3181 gttgcccgcc gtctggccga ggccgcaggt ggaggtctcc ttgcagttcg cagcgagctc
     3241 ctcgaggagc tcgatgtccc cttccttccc ctggcccttc gtgatccgcg tcaggatctc
     3301 gagcatcctc ttcgtcccga tccggcacgg cacgcacttg ccgcagctct cgtcctggac
     3361 gaagtccatg aagaagcggg cgacgtcgac catgcacgag tcctcgtcca tgacgatgag
     3421 gccgcccgac cccatgatcg tcccgaggtg gacgagggag tcgtagtcga tcggggtgtt
     3481 caggtgctcg cgcgtcaggc acccgcccga cgggccgccc gtctgcgccg ccttgaagcg
     3541 cttccccttc gggatgccgc cggcgatgtc gtggacgacc tcgccgagcg tgatccccat
     3601 cgggacctcg acgatgccgg tgttctcgac cttgccggcg agagcgaaga ccttcgtgcc
     3661 ggtgctcttc ggcgtcccga gcgaggcgta ccaggcgccg ccgttcttca cgatcgtcgc
     3721 gacgttcgcg agcgtctcga cgttgttgat gatcgtcggc ttcccgaaga tgccgcgctc
     3781 gaacgggaac ggcggcttct gccgcggctc gccgcgcttg ccctcgacgg agtgcatgag
     3841 cgcggtctcc tcgccgcaga cgaaggcgcc ggcgccgatc cggatctcga cgtcgaaggc
     3901 gaaggagctc cccatgacgc tcggcccgag gaagccccac tcgcgggcct ggcggatcgc
     3961 ggcctcgagc cgctcgacgg cgatcgggta ctcggccctg acgtagacgt agccctggct
     4021 ggcgccgatc gcgtagcccg cgaggatcat cccctcgagg acggagtgcg ggtccccctc
     4081 gaggagggat cggtccatga aggcgccggg gtccccttcg tccgcgttgc agacgacgat
     4141 cttttcgttc cccttcgcct tgaagccggc ctgccacttg gcgccgaccg ggaagccgcc
     4201 gccgccacgg ccccggaggc ccgaggcgcg catctcctgg atgacctgct cgcccgtcat
     4261 cgtcgagagc gccttccaga ggccctggta gccgtcggag gcgatgtagg cctcgatcga
     4321 ggcgtacggc atcgagccgc agcgggccgt cgcgatcttg acctggcgct tgaagaactc
     4381 gatgtcggag aggagcggga cgggctgctt cgtctcgggg tcgacccagc agtgctgctc
     4441 gacgacctcg cggtggacga ggtgctgccg cacgatgccc ttcatgttct cgggcttgag
     4501 ctcggtgtag aggacgccgt cgggctggac gacgagcgtc ggcccgaggt ggcaggagcc
     4561 gaggcagccg gtctcgtgga cggccgtctc gttttcgagc ccggcgtcct tcagggcggc
     4621 gaggagggcg tgcttgacgg cgtggcagcc ggaggagacg cagccggctc cggcgcagac
     4681 gaggagctgg tgcttcttcc cctgctgctc ggcgtgccag cgggcggcga tcgcgtcgag
     4741 gtcggacttg ctcgcgggac gcgtcaggtt cggctcgggg gtcatcttgc gcttcctcat
     4801 cattcgcaga gggcgaggac ccggttcagc ttgtccgggt tgacctgccg cacgaccttg
     4861 tcgttcacga ggagcgcggg cgcgaggccg caggccccga tgcagcgcat gacctcgagg
     4921 gtgaacttcc cgtcgggggt cgtctcgccg acgccgacgc cgagcgtctg cgagaggcgc
     4981 ttgacgatct cgacgccgcc gcggacgtag cacgccgtcc cgaggcagac gcggatcgag
     5041 tattcgccgc gcggcttcgt cgagaagaac gagtagaacg tcgtgacgcc ccagacctcc
     5101 gagacgggga tgtcgagctt ctccgccacg agccgctgga cgtggatcgg gaggtagccg
     5161 tagatcccct gggcgaggtg gaggatctgg atgaggctcc cgggcttgtc gcggaacgct
     5221 tcgacgacct ccgcgatgcg ggcgagcttc tcctcctcgg tcgcttcgcc gcacgcgcag
     5281 gcggtggccg gggacggagg gggagacgag acctggacct gcatggagcc tcctcaggcg
     5341 gggcgcgccg gc
//
LOCUS       DAOONL010000189         5264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_18832_length_5264_cov_9.961221, whole genome shotgun sequence.
ACCESSION   DAOONL010000189 DAOONL010000000
VERSION     DAOONL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18832_length_5264_cov_9.961221"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLP50_16320"
     CDS             <1..398
                     /locus_tag="PLP50_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA53158.1"
                     /translation="ARMGAIAGFAEIEGFLDAPVRTYSTGMSARIAFATATDVDPDIL
                     LVDEMLSVGDERFVAKCQERMAGFRERGKTFFLVSHSLPVVEKTCHRAIWLLDGEVVA
                     DGEPKDVCDRYREWARNGSGGATAENRDR"
     gene            683..1711
                     /locus_tag="PLP50_16325"
     CDS             683..1711
                     /locus_tag="PLP50_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53159.1"
                     /translation="MSSLAALQRRCLFGASLALAAVLLPLASGTARGDELQPNLRKRV
                     EEVLAGQRPLSEVRLEVVGGRPDRRGVVVYGSGVGIWNQEKQFLLSPEEHKELLKRTL
                     AAGLYEMPERPRPGKEPRPAPDAPSAPVIIRAVSVKVGELERTVAQNDRVRTLPALEA
                     LVGELFGLCEKPASRGTGASSLTDGLAKIARGKLAIETLQLVLSIPPVAAGAKGPAEK
                     GVLVVLDGGALTWTGQDAGRAGTAVPLPADVERLRDLAGTLAEAGIESFPVNLYRARY
                     LDLNARALGHAKTIQARAFAGMDPAKHSAHQAALDKVIRAILALGPGPATVPATPQGG
                     GRPAEGAR"
     gene            1888..>5264
                     /locus_tag="PLP50_16330"
     CDS             1888..>5264
                     /locus_tag="PLP50_16330"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPA53160.1"
                     /translation="MRISQKSLLAVVTVASVLLAGALSAEMLPEKRSGRAEVMYPVKV
                     DVSPALRSIPIPPAKPVENREVPNKTTSMRKEVLGTSERIQTTPGVPNTPDPLAGWPG
                     LGSDENQTIIGGRLMPPDTQGDIGKDHYVQWNNLVFAIWDKSGNKVFPAQPGPVAAPG
                     DLLWDGFGGPCETYNDGDPITLWDPLAERWVMSQFAVSMPSAPFYQCVAVSTTSDPTG
                     QWYRYAYAWPGNRFPDYPKLGVWTDGYYITTNDFNSSLTSFVGITAGAFERDKMLDGL
                     PAGFVYFQMTDGGARDYSALPADLDGNMAPPAGSPGLIGEFQDKTWFTPNLPTDQIWL
                     YKLTLDWANPANSTFGIGATHEPDFQLAISDVSENCGGRDCVPQGSSAQKVDDIDFRI
                     MHRLAYRNLGSAEALVASMTVGAGGRAGVWWGEFQNNGAGWATRQDGVYAPADSMHRW
                     MPSIAMDTNGNIMLGYSASSTTIYPSVRYTGRLAEDPLGQMGDEKEMVVGAGAQSGGN
                     RWGDYSMMTIDPVDGCTFWYTQEYIAASGNFDWDTWIGNMKFSTCSAGPAGILEGYVK
                     STAGGDPIAGASVQIGASYSTTTNASGFYQITVPVGTYDVTASKFGFDTATALAQEVL
                     EDATTTVADLLLTPVGTYAVDGFVTAAEHNWPLWARIEVRQAGNLVQTVYNSPWNGYY
                     EIPELPNGFTYEFTVHSMYTGYLDEVRPVTIAAGDQIQSFSLTPASGNPAYTCYLVGG
                     VNEDFNAGFPPLGWTVKNNGSVANNIWKLNTVWGRANETGGTGTAAAADSDKAGSGSG
                     PFDTELWSPPILMPADPRNLKFKQSYRQFTGETGTVDVSTNGGNTWTNLLTITTTSTA
                     EQTVDMTAYAGQLIVLRWKFVCPSWSYYWQVDDVRTETIPPPPPPPIYSEGFEDVTFP
                     PAGWAVINADGGGTTWVRNTTASYVRTGTGSARHSYSSAANQIGWLVTAPIAVPVGAA
                     PLTFWEHTQFPGDYESHTLWACTTGCTAPPTNFTQIAEFPAPVASFRQQSVSLAAYAG
                     GSVTLAFRYEGNNADAWAIDDILIPSAPPAPVTVDSPNLVCESVAGTLVAGFVTDANS
                     GAGVVGADVVRDLGGLAKTVAATGNLPDGFYYMFSPIP"
BASE COUNT          852 a         1774 c         1891 g          747 t
ORIGIN      
        1 aggcgcggat gggcgcgatc gcggggttcg cagagatcga gggcttcctc gacgctccgg
       61 tgaggacgta ctccacaggg atgtcggcgc gcatcgcctt cgcgaccgcg accgacgtcg
      121 atcccgacat ccttctcgtc gacgagatgc tctccgtggg cgacgagcgc ttcgtcgcga
      181 agtgccagga gcgcatggcc ggcttccggg agagggggaa gacgttcttc ctcgtcagcc
      241 actcgctccc ggtcgtggag aagacctgcc accgcgcgat ctggctcctc gacggcgagg
      301 tcgtggccga cggcgagccg aaggacgtct gcgatcgcta ccgcgagtgg gcgcgcaacg
      361 ggtcgggcgg ggcgacggcc gagaaccggg accgctgagg gccggaaagg ggcggacgcc
      421 gccgatccgt cgcccgggcc gcccgaaagc gtgggaaggc agaggatcgt gcttgacgcc
      481 atccgcgagc gcggcatatc ctggctcggt gatccagaca gaccggggtt ccgggtcggg
      541 gaggagcgga cgccatcaag gtccgcggcc ccctgtccgc gcgagtaggt gcctgccggt
      601 cgcccggtag gcgggagggc gagtcctgtc gaagccggct aacgtgagag gaagagagcc
      661 gcgcgcgcgc ggctcggtgg cgttgagttc tctggccgcg ctccagcgga ggtgcctctt
      721 cggcgctagc ctcgcgctgg cggccgtact tctccctctc gcgagcggca cggcgcgggg
      781 cgacgagctc caaccgaacc tgcggaagag ggtcgaggaa gtgctggcag gccagcggcc
      841 gctttccgag gtgcggctcg aggtcgtggg cggccgcccg gaccgtcggg gcgtcgtcgt
      901 gtatggctcc ggggtgggaa tctggaacca ggagaagcag ttcctcctct cgccagagga
      961 gcacaaggag ctcctgaagc ggaccctcgc cgccggcctg tacgagatgc cggagcgccc
     1021 gcggcccggg aaggagcccc gacccgcgcc ggacgcgccg agcgcgccgg tgatcatccg
     1081 ggcggtcagc gtgaaggtgg gcgagctcga gcggaccgtg gcgcagaacg accgcgtccg
     1141 gacgctgccc gcgctggaag cgctcgtcgg cgagctcttc gggctgtgcg agaagccggc
     1201 gagccggggg acgggcgcct cgagcctgac cgacggcctc gccaagatcg cccgcgggaa
     1261 gctggccatc gagactctgc agctcgtgct cagcattccg ccggtcgcgg cgggagcgaa
     1321 ggggccggcg gagaagggcg tcctcgtcgt actcgacgga ggtgcgctga cgtggacggg
     1381 gcaggacgcg gggcgcgccg ggacggccgt tcccctgccg gccgacgtcg agcgactgcg
     1441 cgacctggcc gggacgctcg cggaggccgg gatcgagagc ttccccgtga acctctatag
     1501 ggcgcgctac ctcgacctga acgccagggc gctcgggcac gcgaagacga tccaggcccg
     1561 ggcgttcgcc gggatggacc cggcgaagca ctccgcgcat caggcggcgc tggacaaggt
     1621 gatccgcgcg atcctcgcgc tcggccccgg cccggcgacc gttcctgcga caccgcaggg
     1681 cggcggaagg cccgcggagg gcgcgcgatg agtccgtttc cggtcgtccg cgtcgccgcc
     1741 ggtcgcggcg cgcgggctct cgtcctcttc aacggaaagt ctcccccctc gagccgagga
     1801 gggagtttcg atcgccctgg aacgaccacc gcggaactgc cccagaccac ccggacatcc
     1861 agtgatgcaa ccaggagagg aggaacagtg aggatcagcc agaagagcct tctagccgtc
     1921 gtcacggtcg ccagcgtgct gctggcgggc gcgctgtcgg cggagatgct ccccgagaag
     1981 cgcagcggcc gagccgaggt gatgtacccg gtgaaggtcg acgtctcgcc cgcgctgcgg
     2041 agcatcccga tcccgccggc caagccggtg gagaaccgcg aggtcccgaa caagacgacc
     2101 tcgatgcgca aggaagtcct gggcacgtcg gagaggatcc agacgacgcc cggcgtgccg
     2161 aacacgcccg acccgctggc ggggtggccg ggcctgggga gtgacgagaa ccagacgatc
     2221 atcggcgggc ggctgatgcc gccggacacc cagggcgaca tcgggaagga ccactacgtc
     2281 cagtggaaca acctggtttt cgcgatctgg gacaagtcgg gcaacaaggt gttcccggcg
     2341 caaccgggcc ccgtggcggc cccgggagac ctcctttggg acggtttcgg cgggccgtgc
     2401 gagacctata acgacggcga cccgatcacg ctgtgggacc cgctggccga gcgctgggtg
     2461 atgagccagt tcgcggtctc gatgccttcg gcgccgttct accagtgcgt cgcggtttcg
     2521 acgacttcgg acccgacggg tcagtggtat cggtacgcgt acgcgtggcc gggcaaccgc
     2581 ttcccggact acccgaagct gggtgtctgg acggacgggt actacatcac gacgaacgac
     2641 ttcaactcgt cgctgacgtc cttcgtgggg atcacggcgg gcgcgttcga gcgcgacaag
     2701 atgctggacg gcctcccggc ggggttcgtc tacttccaga tgaccgacgg gggcgcccgg
     2761 gactactcgg ccctcccggc cgacctggac gggaacatgg cgccgccggc gggctcgccg
     2821 gggctgatcg gtgagttcca ggacaagacc tggttcacgc cgaacctgcc gaccgaccag
     2881 atctggctgt acaagctgac gctggactgg gcgaaccctg cgaactcgac gttcggtatc
     2941 ggggcgaccc acgagccgga tttccagctc gcgatctccg atgtgagcga gaactgcggc
     3001 gggcgcgact gcgtcccgca gggctcgtcc gcgcagaagg tcgacgacat cgacttccgg
     3061 atcatgcacc ggctggcgta ccgcaacctc gggtcggcgg aagcgctggt ggcgagcatg
     3121 acggtcgggg cgggtggccg cgccggcgtg tggtggggcg agttccagaa caacggcgcg
     3181 gggtgggcga cgcggcagga cggcgtctac gcgccggccg acagcatgca ccggtggatg
     3241 ccctccatcg cgatggacac caacggcaac atcatgctcg gctactcggc gtcctcgacg
     3301 accatctacc cgtccgtgcg ctacacgggc cgcctggcgg aggacccgct cgggcagatg
     3361 ggcgacgaga aggagatggt cgtcggggcg ggtgcgcagt ccggcgggaa ccgctggggc
     3421 gactactcga tgatgacgat cgacccggtg gacggctgca cgttctggta cacccaggag
     3481 tacatcgccg ccagcgggaa cttcgactgg gacacctgga tcggcaacat gaagttctcg
     3541 acctgctcgg cggggccagc ggggattctc gagggctacg tgaagagcac ggccggcggt
     3601 gacccgatcg cgggcgccag cgtgcagatc ggcgcgtcgt actcgacgac gacgaacgcg
     3661 tcgggcttct accagatcac ggtgcctgtg ggcacctacg acgtgacggc gtcgaagttc
     3721 gggttcgaca cggccacggc gctggcgcag gaggtcctgg aagacgccac gacgaccgtc
     3781 gccgacctgc tgctgacgcc tgtgggcacg tacgcggtcg acggcttcgt gacggcggcc
     3841 gagcacaatt ggccgctttg ggcgcggatc gaggtccggc aggccgggaa cctcgtccag
     3901 accgtctata actcgccgtg gaacgggtac tacgagatcc cggagctgcc gaacggcttc
     3961 acgtacgagt tcacggtcca ctcgatgtac acgggctacc tggacgaggt ccgtccggtg
     4021 acgatcgccg ccggcgacca gatccagagc ttctccctca cgccggcctc cggcaacccg
     4081 gcgtacacct gctacctcgt cggcggggtc aacgaggact tcaacgccgg cttcccgccg
     4141 ctcggctgga ccgtcaagaa caacggctcg gtcgcaaaca acatctggaa gctcaacacc
     4201 gtctggggcc gggcgaacga gaccggcggc actggcaccg cggccgctgc cgactcggac
     4261 aaggccggct ccggctccgg gccgttcgac accgagctct ggtctccccc gatcctgatg
     4321 ccggcggatc ctcggaacct gaagttcaag cagagctacc gtcagttcac cggcgagacg
     4381 gggaccgtcg acgtctccac gaacggcggg aacacctgga cgaacctcct cacgatcacg
     4441 acgacctcca cggcggagca gaccgtcgac atgacggcct acgccgggca gctgatcgtc
     4501 ctcaggtgga agttcgtgtg ccccagctgg tcctactact ggcaggtcga cgacgtgcgg
     4561 acggagacga tcccgccgcc gccgccgccg ccgatctact ccgaaggctt cgaagacgtg
     4621 accttcccgc ctgcgggttg ggccgtcatc aacgctgacg gcgggggaac gacctgggtg
     4681 cggaacacga cggcgtccta cgttcggacg ggcaccggtt ccgctcgcca cagctactcc
     4741 tcggcggcca accagatcgg gtggctcgtg acggcgccga tcgccgtccc ggtgggcgcg
     4801 gctccgctga ccttctggga gcacacgcag ttcccgggcg actacgagag ccacaccctc
     4861 tgggcctgca cgaccggctg caccgccccc ccgacgaact tcacgcagat cgccgagttc
     4921 ccggccccgg tcgcgtcgtt ccggcagcag agcgtgagcc tcgccgccta cgcgggcggc
     4981 agcgtcactc tcgcgttccg ctacgaaggg aacaacgcgg acgcctgggc gatcgacgac
     5041 atcctcatcc cgtcggcccc gccggcgccc gtgaccgtcg attctccgaa cctcgtctgt
     5101 gagtcggtcg ccggcaccct ggtcgccggt ttcgtcacgg acgcgaattc gggcgcgggc
     5161 gtcgtcggag cggacgtcgt gcgggacctc ggcggcctgg ccaagacggt ggcggccacg
     5221 gggaacctcc ccgacggctt ctactacatg ttctcgccga tccc
//
LOCUS       DAOONL010000190         5198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19150_length_5198_cov_18.053471, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000190 DAOONL010000000
VERSION     DAOONL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19150_length_5198_cov_18.053471"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLP50_16335"
     CDS             <1..215
                     /locus_tag="PLP50_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPA53161.1"
                     /translation="QPQVAILNIGTIEKRAKVVEMPDGSDAIAVRTMGYLALSFDHRL
                     VDGAEADAFMGVVKASLESGSFGDLE"
     gene            309..1865
                     /locus_tag="PLP50_16340"
     CDS             309..1865
                     /locus_tag="PLP50_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HPA53162.1"
                     /translation="MASAGSEYDDVPYLAPSFANTYPDALGVAAILRGLDAPPLHGAR
                     VLELGCARGANLVPMAWSMPDSEFVGVDLSRRQIEAARERAAAAGTANVTFHAMDLRD
                     VDDSFGAFDFVVAHGLFSWVPREVQESLLEVCARRLSPNGIVYLSYNTLPGWSAGVMF
                     REMVLYRVRGIADPAERLREARAFLRFLAGATRPKETIWAAQLDEHASYFEQAHDWYL
                     LHDDLEAENNPVYFHEIVERAGRHGLQYVTEERSLTSGEVLAPEALAQLDRFAANRIE
                     REQVVDFACSRRFRRSLFCRAGRPLPTGPEPGRLAACRLRSRVKPVDPSADPLAPGSE
                     SFLGLDGLTLETSDVRLRTLLHVLWGVWPGTLEHAAATHVLAEATRRSGGPTPEPPVL
                     DRMVHQAILGQVVSAHLLDPQIAAALPERPSASPVARYEAAAGEPVTHLLNGSGDLDP
                     LDRFVVRLLDGTRTRDDVVASIREAFDAGQLEPTPSGRAAEPGEEPKSAAELADLSLS
                     HLLAACYIHR"
     gene            1866..2630
                     /gene="lipB"
                     /locus_tag="PLP50_16345"
     CDS             1866..2630
                     /gene="lipB"
                     /locus_tag="PLP50_16345"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPA53163.1"
                     /translation="MRRPVDVVSLLPLGPRPYDDVVALQKEAEARAKEGGRETLFLLE
                     HEDVITAGRNAGTDDLHLPAEQLARLGVAFRRSDRGGKLTFHGPGQLVAYPILRLEGP
                     ERDVRRFVRSLEEVLILTAAAFGVTAARSDLPARWSSVWVGNDKLAAIGVHLSDWVTT
                     HGVALNVTTRLDRFELFVPCGIRDGGVTSLERARPDLPPPPLVDVAARFVESFAEVFE
                     RDAVPVSAASSSEELAPPTAAASRSTGPDRSPSNGA"
     gene            complement(2713..3237)
                     /locus_tag="PLP50_16350"
     CDS             complement(2713..3237)
                     /locus_tag="PLP50_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA53164.1"
                     /translation="MPTRRTRTPANAGPRWGAELTRIELLRLREVLRLAGLTLTPTRT
                     AVLWGLQRSATPATLAELSESLAARGWARSAVSRAVNELVGARLVTRTAPAGRGVELL
                     AAAAEPELPEGTEYFLCEGCSVNECLEEAHVLATAGGSGPKALPRAEVTDMVESLCRR
                     REAQKAPPLPVRAR"
     gene            complement(3376..>3771)
                     /locus_tag="PLP50_16355"
     CDS             complement(3376..>3771)
                     /locus_tag="PLP50_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53165.1"
                     /translation="APPVDLAAQADRSWEGLVRRNDGIVLFKGRWDNGRLSFKEEKLS
                     WLDAKDEKKNLILPAKSIKEQFLVCLKGAPNEETECFEWGVKTENGEYRFRDVGWKRS
                     DSVKPQELYDFMKAIYPNLVSSKYPADEK"
     assembly_gap    3772..3871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3872..4298)
                     /locus_tag="PLP50_16360"
     CDS             complement(<3872..4298)
                     /locus_tag="PLP50_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53166.1"
                     /translation="MSLMKLSGALGAFVVATTLTIPGAALSQDAPPPPPAEIAQVAPA
                     PPPPPAPKPFEEIIKLWKGNLSEEFIKRKIESEAVIYQLSADDIVLSKAANLPESIIE
                     AMMKTAQRPMAAPAAVAPVTSPAAPVPAAVAPVAPAAPPV"
     gene            4460..4876
                     /locus_tag="PLP50_16365"
     CDS             4460..4876
                     /locus_tag="PLP50_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53167.1"
                     /translation="MALRPAASARPLAALALAVSLLPFTLPAGPAAPCPASVPSPASG
                     CCPSSAPPACPACPTEGRPSAPTAPKRAAACCSPAAVLPRASRLIEAEAPSARADGLV
                     RVRSSRSTARAEADARRSSVVAASPPPRLLACTFRN"
     gene            5005..>5198
                     /locus_tag="PLP50_16370"
     CDS             5005..>5198
                     /locus_tag="PLP50_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53168.1"
                     /translation="MRQIASLALALALVAAPAALAGPECAGHAKAASCCAKKDGADAG
                     KKCDPASCKKDAAKCTHADKA"
BASE COUNT          678 a         1956 c         1755 g          709 t
ORIGIN      
        1 accagccgca ggtcgcgatc ctgaacatcg ggacgatcga gaagcgggcg aaggtcgtgg
       61 agatgcccga cggcagcgac gccatcgccg tccggacgat gggatacctc gccctttcgt
      121 tcgaccaccg cctcgtcgac ggcgccgagg ccgacgcatt catgggcgtc gtcaaggcga
      181 gcctggagag cgggagcttc ggcgacctgg aatagaaggg acaccaccac gagcggagcg
      241 acgaacgggg ggccacggcc ccccgttcgt cctcgtggag cccgggccgg cagcgagaag
      301 gagcgacgat ggcttcagca ggatcggaat acgacgacgt cccatacctc gccccgtcgt
      361 tcgcgaacac ctaccccgac gccctcggcg tggccgcgat cctgcgcggc ctcgacgcgc
      421 ctccccttca cggggcgcgc gtcctcgagc tcggctgcgc acggggcgcg aacctcgtgc
      481 cgatggcctg gtcgatgccc gactccgagt tcgtcggcgt cgacctctcg cgccgccaga
      541 tcgaagccgc ccgcgagcgg gccgcggcgg ccggcacggc caacgtcacg ttccacgcca
      601 tggacctgag ggacgtcgac gactcgttcg gcgccttcga cttcgtcgtg gcgcacggcc
      661 tcttctcctg ggtcccgagg gaggttcagg aatccctcct cgaggtctgc gcgcggcgcc
      721 tctccccgaa cgggatcgtc tacctcagct acaacaccct cccgggctgg agcgcggggg
      781 tgatgttccg ggagatggtc ctctaccgcg tccgcgggat cgccgacccc gcggagcgtc
      841 tccgggaggc gagggccttc ctccgattcc tcgccggggc gacgaggccg aaggagacca
      901 tctgggcggc gcagctggac gagcacgcgt cgtacttcga gcaggcgcac gactggtatc
      961 tcctccacga cgacctcgag gccgagaaca accccgtcta cttccacgag atcgtcgagc
     1021 gggccggacg gcacgggctc cagtacgtca cggaagagcg ttccctcacc tcgggcgagg
     1081 tcctggctcc cgaggccctc gcgcagctcg accgcttcgc ggcgaaccgg attgagcgcg
     1141 agcaggtcgt cgacttcgcc tgcagccgcc gtttccgccg gagcctcttc tgccgcgccg
     1201 gacggcccct ccccaccggc ccggagcccg gccgcctcgc cgcctgccgt ctccggtcac
     1261 gcgtgaagcc ggtcgacccg tcggccgacc cgctcgcgcc gggctcggag agcttcctcg
     1321 ggctcgacgg gctgacgctc gagacctccg acgtccgcct gcgcaccctc ctccacgtcc
     1381 tctggggagt ctggccgggg acgctcgagc acgccgccgc gactcacgtc ctcgccgaag
     1441 cgacccgccg ctccggcggc ccgacgcccg agccgcccgt cctcgaccgc atggtccacc
     1501 aggccatcct cggccaggtc gtctccgccc acctcctcga cccgcagatc gccgcggccc
     1561 tgcccgagcg cccgtccgcc tccccggtcg cccggtacga ggccgcggcc ggcgagccgg
     1621 tgacccacct tctcaacggc tcgggcgacc tcgacccgct cgaccgcttc gtcgtccggc
     1681 tcctcgacgg cacgcggacc cgggacgacg tcgtcgcctc gatccgcgag gcgttcgacg
     1741 ccggccagct cgagccgacc ccgtcggggc gcgccgcgga gccgggcgag gagccgaagt
     1801 ccgccgcaga gctcgcggac ctctccctct cccacctcct cgcggcctgc tacattcacc
     1861 gctgaatgcg ccgccccgtc gacgtcgtct ccctcctgcc gctcggtccc cgcccctacg
     1921 acgacgtcgt cgccctccag aaggaggcgg aggcgcgtgc gaaggagggc ggacgggaga
     1981 ccctcttcct tctcgagcac gaggacgtca tcaccgccgg ccggaacgcc ggcaccgacg
     2041 acctgcacct cccggccgag cagctcgccc ggctcggcgt ggcgttccgg cgctccgacc
     2101 ggggcggaaa gctgacgttc cacggaccgg gccagctcgt cgcctatccg atcctccgcc
     2161 tcgaagggcc cgagagggac gtccgccggt tcgtgcgtag cctggaggag gtcctgatcc
     2221 tcacggcggc cgccttcggc gtcaccgccg cgcggagcga cctccccgcg cgctggtcct
     2281 ccgtctgggt cggcaacgac aagctcgccg ccatcggcgt ccacctctcc gactgggtca
     2341 cgacgcacgg cgtcgccctc aacgtcacga cccgcctcga caggttcgag ctcttcgtcc
     2401 cgtgcggcat ccgggacggc ggagtcacct ccctcgagag ggcgcgcccc gaccttccgc
     2461 ccccgcccct cgtggacgtc gcggcccgct tcgtcgagag cttcgcggag gtcttcgagc
     2521 gcgacgcggt tcccgtgtcg gcggcctcct cttcggagga gctcgccccc ccgaccgccg
     2581 cggccagccg ctcgaccggc ccggaccgat cgccctcgaa cggcgcctga agcggttcag
     2641 ggacccgaag gacgaacggc tcccgcagag cggggcgtct cgccgatcac ggaatcggcg
     2701 gccaagggcg gctcagcgcg cccggacggg gagggggggg gctttctgcg cctcgcggcg
     2761 ccggcagagg ctctcgacca tgtcggtcac ctcggcccgc gggagggctt tcggtccgct
     2821 gccgcccgcg gtggcgagga cgtgggcctc ctcgaggcac tcgttcacgg agcagccctc
     2881 acaaaggaag tactccgtcc cttcgggaag ctcgggctcg gccgccgcgg cgaggagctc
     2941 gacgccccgc cccgcgggcg ccgtccgcgt cacgagccgg gccccgacga gctcgttcac
     3001 ggcccgggag acggcgctgc gcgcccagcc gcgagcggcg agcgactcgg acagctcggc
     3061 gagggtcgcg ggcgtggccg aacgctgcag gccccagagg acggcggtgc gggtcggagt
     3121 gagcgtgaga ccggcgaggc ggaggacttc ccgcagccgg agcagctcga tgcgcgtcag
     3181 ctcggctccc cagcggggac cggcgttcgc aggagttcgc gtccggcgcg tcggcacgcg
     3241 cgaggtgtaa catgggaggt ttgccgcggg caagcgggcg cgggactccg cgggcagatt
     3301 ccggagacgg aaacgccctc cccccgggcg gagagagggc gtcggatgcg gcttccggag
     3361 ccgtaagtgt cgagcctact tctcgtcggc cgggtacttc gacgagacga ggttcgggta
     3421 gattgccttc atgaaatcgt agagctcctg gggcttgacg ctgtcggagc gcttccagcc
     3481 gacgtcgcgg aagcggtact cgccgttctc cgtcttcacg ccccactcga agcactcggt
     3541 ctcctcgttc ggagccccct tcaggcagac gaggaactgc tccttgatcg acttcgccgg
     3601 aaggatgagg ttcttcttct cgtccttcgc gtcgagccag gagagcttct cttccttgaa
     3661 ggagagcctg ccgttgtccc agcggccctt gaagaggacg atcccgtcgt tccggcggac
     3721 gaggccttcc caggagcgat cggcctgggc ggccaggtcg accggcgggg cnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgaccggcg gggccgccgg ggcgaccggc
     3901 gcgacggcgg ccggcaccgg ggccgccggg gaggtgacgg gcgcgacggc ggccggggcg
     3961 gccatcgggc gctgcgccgt cttcatcatc gcctcgatga tcgactcggg aaggttcgcc
     4021 gccttggaga ggacgatgtc gtcggcggag agctggtaga tcaccgcctc cgactcgatc
     4081 ttccgcttga tgaactcctc ggagaggttc cccttccaga gcttgatgat ctcctcgaac
     4141 ggcttcggag ccggcggcgg gggcggggcg ggggccacct gggcgatctc ggccggcggc
     4201 gggggcgggg cgtcctggga gagggcggcg ccggggatcg tcagcgtcgt cgcgacgacg
     4261 aacgcgccca acgcgccgga gagcttcatc agggacatct gtgcgtacct cctctgctcg
     4321 accgggagcc ggggtcgacc gcgcgggcga aacgctcccg atcctcgctg agcgtccatt
     4381 ctcgctcacg aaccggtcgg gcgcacgacc cccgtgcggg ggcggtcccc tggcacgcgt
     4441 tttcgatacg atccctccga tggccctccg ccccgcagct tcggcacgac cgctcgccgc
     4501 gctggcgctc gcggtgtctc tcctgccgtt cacgctcccg gcagggcctg cggcgccctg
     4561 ccccgcctcc gtgccgtcgc ccgcgtcggg ctgctgcccc tcgagcgccc cgcccgcctg
     4621 cccggcctgt cccaccgagg gtcgcccttc ggcgccgacg gcgccgaaga gggcggccgc
     4681 ctgctgctcc cccgcggcgg tcctcccccg cgcctcgagg ctgatcgagg cggaggcccc
     4741 gtcggcgcgg gccgacggcc tcgtccgggt ccgatcctcc cgctccacgg cgcgcgcgga
     4801 ggccgacgcc cggcggtcgt ccgtcgtcgc cgcgtctcct cctcctcgtc tcctcgcctg
     4861 cacgttccgg aactgaccgg tcccgcggga ggcaccacgt cttccgcgcc gcgagagcgg
     4921 atcgcctccg cccgccgacc gatggaaggg gcgcggcccg aagccgcgcc ccacgagaaa
     4981 cgaagaaaca agaaggagaa cctcatgcgt cagatcgctt ccctcgccct cgccctcgcc
     5041 ctcgtcgccg ccccggccgc gctcgccggc ccggaatgcg ccggccatgc caaggccgcc
     5101 tcctgctgcg cgaagaagga cggcgccgac gccggcaaga agtgcgaccc cgcgagctgc
     5161 aagaaggacg ccgcgaagtg cacccacgcc gacaaggc
//
LOCUS       DAOONL010000191         5186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19217_length_5186_cov_17.926330, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000191 DAOONL010000000
VERSION     DAOONL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19217_length_5186_cov_17.926330"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLP50_16375"
     CDS             <1..543
                     /locus_tag="PLP50_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPA53169.1"
                     /translation="PMPSEGGHASFAPRNDEEIELLKTLRLRHGHVSWERVVSGPGLG
                     TLYRFERQLSGVPEPEWLAREIAASRDAAPVVSRAALDETDPVCQRTLHRFVSLFGAE
                     AGNLALKALATGGVFVGGGIAPKILPLLRDGFFTAFTAKGRFAPVLARIPIRVIRNDS
                     CAILGAARAAARAARGEVTT"
     gene            540..1577
                     /locus_tag="PLP50_16380"
     CDS             540..1577
                     /locus_tag="PLP50_16380"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPA53170.1"
                     /translation="MTTSYGTIGTRLHRHLFETERRYPHATGELSALLSQLALAGKRI
                     ARVLSRAGLAEVLGPSGVTDVQKERLQRLDALANEIFLEAFAYGQLVPTVVTQQMDLP
                     ARLPENEAVAASAGKYVVFVDPLDGASNLDINGAVGSIFSVRRIGGSGPILSEAELLA
                     RISQQQVVAGYIVYGPATMLVYTAGPKSGVQGFTLDPGIGEFLLSHPDVKVPRRGPYY
                     ACNEGGLEAWPEAPRRFVESLRVPDPARGRPLSTRYSGALVVDVHRILLRGGVFLSPG
                     TDSEPDGKLRLMYEAAPLAAVVEAAGGMAIDGARRIVSIFPEENHQRTPLYIGSREDV
                     EELEREIAAVR"
     gene            1649..2149
                     /locus_tag="PLP50_16385"
     CDS             1649..2149
                     /locus_tag="PLP50_16385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPA53171.1"
                     /translation="MKKTTVLLCALAIAGATPALAQKPAAAKSASEKAVAPKEPAPEA
                     EKKESKMVKTESGLGYEDLVVGTGASPETGQTCVMHYTGWLFENGAKGAKFDSSVDRN
                     RPFEFPIGRGRVIKGWDEGVATMKVGGKRTLLIPPQLGYGDRGAGNVIPPGATLLFEV
                     ELLGVK"
     gene            complement(2142..3998)
                     /locus_tag="PLP50_16390"
     CDS             complement(2142..3998)
                     /locus_tag="PLP50_16390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA53172.1"
                     /translation="MQLPPGTRLGPYEVLAPIEAGGMGSVYRGRDGRLLRDVAVKVLP
                     VGMMRDERAVERFLREARAAAALSHPNIVAIHDVGDASVAIPGPFGGSETRIRYLVEE
                     YVDGGSLRRALKGSWRPDVTTVVGWAVGILRALSAAHAKGLIHRDLKPENVLVDGQGN
                     AKIADFGLVRWIYPQSAAPSPREEERDGPATLTQTGFVVGTLGYMSPEQVRGEPVDGR
                     SDLFAFGVLLFELLTGSSPFARSSADAALDAVLNHEPSSLTVLVAGVPDRLARVVAWC
                     LEKQPGKRPASARDVLSHLESIQRELAAAPLEPTTELPPLPPPGADRRGPSPVAWLAG
                     ALAVLLAFAGGFAAGRSRPAPSADANLTGSWTAQAVALPASSPSEAALLPSGRKAILG
                     SGELLDTTTGALRPRALGGALTGLAVGRTGSAVAATSAAGVVEAPLPAGTPLRMLVPG
                     ASEPAISRDESSLAYVRREGGTSELWVARRDASEARRVARAEATFWAWPAFSADGDSL
                     LFYDVERSGPHAGTGYLFSVRIADGTPVPFGPDVRLDPRGRPAVTRDGSVAVLEAGSR
                     RALLLEREGRSSTALPFGAGLVLLTASPEGGTLATRSESGPLVLWRRVPGAT"
     gene            4088..4504
                     /locus_tag="PLP50_16395"
     CDS             4088..4504
                     /locus_tag="PLP50_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPA53173.1"
                     /translation="MIHLSRTIHFNAAHRLFRRDRSEDWNRATYGAASNPSGYGHNYA
                     LEVAVEGEIDPVDGMVLNLLDLDRILKEEVDRPLDHRNLNEEIPEFRQTVPTAENLAR
                     WIWDRVDARLVRDGCRCRVASLTLTVTPTFRVELTR"
     gene            complement(4575..>5186)
                     /locus_tag="PLP50_16400"
     CDS             complement(4575..>5186)
                     /locus_tag="PLP50_16400"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPA53174.1"
                     /translation="EVLALREEFRGRIDVLLGIEADFAEGHEEGLRELLAPYPWDVVL
                     GSVHFVAGGWIDAPGAAARHEREGSANLWNEYFRLVAKAAGNGTYDVMTHLDLPKKFG
                     HRRPAECVAAEEAAVEAIRAAGVAVEVSSAGLRKPVGEEYPEPALLSKLVAAGVPLVF
                     SSDGHAPAEVGWGLPQVVAEAKAAGATGHVLFRKRRAERHPLR"
BASE COUNT          731 a         1951 c         1837 g          667 t
ORIGIN      
        1 ccgatgccgt cggagggcgg gcacgcctcc ttcgcgccgc ggaacgacga ggagatcgag
       61 ctcctgaaga cgctccgcct ccggcacggc cacgtctcct gggagcgggt cgtctccggg
      121 ccgggcctcg gcaccctcta ccgcttcgag cgccagctct cgggcgtccc cgagccggag
      181 tggctcgcgc gcgagatcgc cgcctcccgc gatgcggccc cggtcgtcag ccgggcggcg
      241 ctcgacgaga cggaccccgt ctgccagcgg acgctccacc gcttcgtctc gctcttcggc
      301 gccgaggcgg ggaacctcgc cctgaaggcg ctcgccacgg gaggcgtctt cgtcggcggc
      361 gggatcgccc cgaagatcct cccgctcctg cgcgacggct tcttcaccgc gttcacggcc
      421 aaggggcgct tcgccccggt cctcgcccgg atcccgatcc gcgtcatccg gaacgactcc
      481 tgtgccattc tcggcgccgc gcgggccgcc gccagggcgg cccgcggaga ggtgacgacg
      541 tgacgacgtc gtatggaacg attgggaccc ggctccaccg gcacctcttc gagacggaga
      601 ggcgctaccc gcacgccacc ggcgagctct cggcgctcct ctcgcagctc gcgctcgcgg
      661 gcaagaggat cgcgcgcgtc ctctcccgcg ccggcctcgc cgaggtgctc ggcccctccg
      721 gcgtgaccga cgtgcagaag gagcgcctcc agaggctcga cgccctcgcg aacgagatct
      781 tcctcgaggc gttcgcctac gggcagctcg tccccacggt cgtcacgcag cagatggacc
      841 tcccggcgcg cctccccgag aacgaggcgg tcgccgcctc ggcggggaag tacgtcgtct
      901 tcgtcgaccc cctcgacggc gcctcgaacc tcgacatcaa cggcgcggtc gggtcgatct
      961 tctcggtccg caggatcggc gggtcggggc cgatcctctc cgaggcggag ctcctcgcgc
     1021 ggatctccca gcagcaggtc gtcgcgggct acatcgtcta cggccccgcc acgatgctcg
     1081 tctacacggc cgggccgaag tcgggtgtcc agggcttcac cctcgacccc gggatcggcg
     1141 agttcctcct ctctcacccc gacgtgaagg tcccgcgccg cggcccctac tacgcctgca
     1201 acgagggggg cctcgaggcc tggcccgagg cgccgcgccg cttcgtcgag tcgcttcgcg
     1261 tcccggaccc cgcgcgcggg cggccgctgt cgacccgcta ctcgggcgcc ctcgtcgtcg
     1321 acgtccaccg gatcctcctc cgcgggggcg tcttcctctc gcccggcacg gactccgagc
     1381 cggacggcaa gctgcggctc atgtacgagg cggcgccgct cgcggcggtc gtcgaggcgg
     1441 cgggcgggat ggcgatcgac ggggcgcggc ggatcgtctc gatcttcccc gaggagaacc
     1501 accagcggac tccgctctac atcgggagcc gcgaggacgt ggaagagctg gaacgggaga
     1561 tcgcggccgt ccggtgacgg aggggcggga gtcgggccga tcgggtaccc ttccgcctcc
     1621 gcgatccgcg gacccgggag gacgaacgat gaagaagacg acggtgctcc tctgcgccct
     1681 cgcgatcgcc ggggcgaccc cggccctcgc ccagaagccc gcggcggcga agagtgcgag
     1741 cgagaaggcg gtcgcgccga aggagccggc ccccgaggcc gagaagaagg agtcgaagat
     1801 ggtgaagacc gaaagcggcc tcggctacga ggacctcgtc gtcgggaccg gcgcgtcgcc
     1861 cgagaccggc cagacctgcg tcatgcacta caccggctgg ctcttcgaga acggcgcgaa
     1921 gggagcgaag ttcgacagct ccgtcgaccg gaaccgcccc ttcgagttcc cgatcgggcg
     1981 cggccgcgtc atcaaggggt gggacgaagg cgtcgccacg atgaaggtcg gcggcaagcg
     2041 gaccctcctc atcccgccgc agctcggcta cggcgaccgc ggggcgggga acgtcatccc
     2101 gcccggcgcc acgctcctgt tcgaggtcga gctcctcggc gtcaagtagc tccggggacc
     2161 cgcctccaga ggacgagcgg cccgctctcc gagcgggtcg cgagcgtccc tccctccggc
     2221 gacgccgtca ggaggacgag tcccgctccg aacggaagcg cggtcgacga gcgcccctcg
     2281 cgctcgagga ggagcgcgcg gcgggagccg gcctcgagaa cggccacgct gccgtcgcgg
     2341 gtgacggccg ggcggccgcg cgggtcgagc cgcacgtcgg ggccgaacgg gaccggggtc
     2401 ccgtcggcga tccggaccga gaagagatag ccggtcccgg cgtgcgggcc gctccgctcc
     2461 acgtcgtaga acaggaggct gtctccgtcg gccgagaacg cgggccacgc ccagaacgtc
     2521 gcttcggccc gtgcgacgcg acgggcttcc gacgcgtcgc gccgggcgac ccagagctcg
     2581 ctcgttcctc cctcgcggcg gacgtacgcc agcgacgact cgtcccggga gatggcgggc
     2641 tccgaggctc cggggacgag catccggagc ggcgtgccgg ccggcagggg cgcctcaacg
     2701 acgccggcgg cggacgtcgc cgccaccgcc gatcccgtcc ggccgacggc gagccccgtc
     2761 agggctcctc cgagggcgcg cggccggagc gcccccgtcg tcgtgtcgag cagctcgccg
     2821 ctcccgagga tcgccttgcg gccgctcggc aggagcgccg cttcggacgg ggaggacgcc
     2881 ggaagcgcga cggcctgcgc cgtccagctc ccggtgaggt tcgcgtcagc gctcggggcc
     2941 ggccgggacc ggcccgcggc gaagccgccg gcgaacgcca ggaggacggc gagcgcgccg
     3001 gcgagccagg cgacgggaga cgggccgcgc cggtccgctc ccggcggcgg gagcggagga
     3061 agctcggtcg tcggctcgag cggagcggcc gcgagctctc gctggatcga ctcgaggtgg
     3121 gacagcacgt cacgggccga ggcggggcgc ttcccgggct gcttctcgag acaccaggcg
     3181 acgacccgcg cgagccgatc cgggacaccc gcgacgagga ccgtcagcga cgacggctcg
     3241 tggttcagaa cggcgtcgag cgccgcgtcg gcgctcgagc gggcgaacgg cgacgagccc
     3301 gtcagcagct cgaagaggag gacgccgaac gcgaagaggt cgcttcgccc gtcgaccggc
     3361 tcgccgcgca cctgctccgg gctcatatag ccgagcgtcc cgacgacgaa gccggtctgc
     3421 gtcagcgttg ccggcccgtc gcgctcctcc tcccgcgggg agggggcggc ggactgcggg
     3481 tagatccagc ggacgaggcc gaagtcggcg atcttcgcgt tcccctgccc gtcgacgagg
     3541 acgttctcgg gcttcaggtc gcggtggatc agccccttgg cgtgcgcggc tgaaagggcc
     3601 cggaggattc cgaccgccca gccgaccacc gtcgtcacgt cgggccgcca gctccccttg
     3661 agcgcgcggc ggagcgagcc gccgtcgacg tactcctcga ccaggtagcg gatgcgcgtc
     3721 tcgctcccgc cgaaggggcc cgggatcgcg accgaggcgt cgccgacgtc gtggatcgcg
     3781 acgatgttcg gatgggagag cgccgcggcc gcgcgcgcct cgcgcaggaa ccgctccacg
     3841 gccctctcgt cgcgcatcat cccgacgggg agcaccttga cggcgacgtc gcggaggagc
     3901 cgtccgtccc gcccgcggta gacggagccc atccctcccg cctcgatcgg cgcgaggacc
     3961 tcgtatggcc cgagtcgggt gccgggaggc agctgcatgc ggcgtccgtc gtctccgtgt
     4021 cgagcggatg atgttcgagg tccgaacggg aggcaacgtc ttcccgccga cactagaatc
     4081 gcgcccgatg atccacctct cccggaccat ccatttcaac gccgcccacc gcctcttccg
     4141 gcgcgaccgc tccgaggatt ggaaccgcgc cacctacggc gccgcctcga acccctccgg
     4201 ctatgggcac aactacgcgc tggaggtcgc cgtcgaaggc gagatcgacc ccgtcgacgg
     4261 catggtcctc aacctcctcg acctcgaccg gatactcaag gaggaggtcg accgaccgct
     4321 cgaccaccgg aacctcaacg aggagatccc ggagttccgc cagaccgttc ccacggccga
     4381 gaacctcgcc cgctggatct gggaccgcgt cgacgcccgc ctcgtccgcg acggctgccg
     4441 ctgccgcgtt gcgagcctca cgctcaccgt caccccgacc ttccgcgtcg agctcacacg
     4501 ataggggggg gtcaggtcta ccatctacac tctacggcgt cgccgtagag tgtagatggt
     4561 agacctgacc ccccctatct gaggggatgc cgttcggccc gtcgtttccg gaacaagacg
     4621 tgtccggtcg ccccggcggc cttcgcctcc gcgacgacct gcgggagacc ccagccgacc
     4681 tcggccgggg cgtggccgtc ggaggagaag acgagaggca ccccggcggc gacgagcttc
     4741 gaaagcagcg cgggctccgg gtactcctcg cccaccggct tgcggaggcc ggcgctcgag
     4801 acctccacgg cgacgccggc cgcgcggatc gcttcgaccg cggcctcctc ggccgccacg
     4861 cactcggccg ggcggcgatg cccgaacttc ttcggcaggt cgaggtgcgt catcacgtcg
     4921 taggtcccgt ttcccgccgc cttcgccacg agccggaaat actcgttcca gaggttcgcc
     4981 gacccctcgc gctcgtggcg cgcggcggcg cccggggcgt cgatccagcc ccccgccacg
     5041 aagtggaccg atccgaggac gacgtcccac gggtacggcg cgaggagctc gcggagaccc
     5101 tcctcgtgcc cctccgcgaa gtccgcctcg atcccgagga ggacgtcgat ccggccgcgg
     5161 aactcctcgc gcagcgcgag cacctc
//
LOCUS       DAOONL010000192         5174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19276_length_5174_cov_16.156085, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000192 DAOONL010000000
VERSION     DAOONL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19276_length_5174_cov_16.156085"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="era"
                     /locus_tag="PLP50_16405"
     CDS             <1..698
                     /gene="era"
                     /locus_tag="PLP50_16405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPA53175.1"
                     /translation="HKPLHRLNRVMLDEAMEAILDVDLVLLVIDAAEAEGAGDRHALE
                     LVGKAKPPRIAVLNKVDLVSKPKLLPRMAALAATGLFDEIVPISAATGDGLEGLPELL
                     LRYLPEGDDLYPAGTVTTSDERTRVAELIREKFLERTREEIPYGLGVVVDEWVVDETK
                     GMTRVTATLVVDKENHKRIVIGLGGQLIRDAGTAARLEIEKTFGRRFFLDLHVVARPG
                     WREDPRFLGTLSS"
     gene            772..1101
                     /locus_tag="PLP50_16410"
     CDS             772..1101
                     /locus_tag="PLP50_16410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPA53176.1"
                     /translation="MQPERRRSHRMRVDGEGPSGVVRGLAPVSVSDLSPGGLRLRLDR
                     ALEAGAVYPLTLFLRGLAVTTAVRVTRCRRDSRSAWEAGGEFLFRDPTDAAAVRRWID
                     ARSTPVP"
     gene            1235..1519
                     /locus_tag="PLP50_16415"
     CDS             1235..1519
                     /locus_tag="PLP50_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53177.1"
                     /translation="MEDVSKDGVCFRLSGPVRPGSTYAFHASLEGLDLAAPIRITRCK
                     AISPAMGGGRGLVYEAGAEFAWERPGDEERVVTWLARRGSAGGLRAELEG"
     gene            1643..3427
                     /locus_tag="PLP50_16420"
     CDS             1643..3427
                     /locus_tag="PLP50_16420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA53178.1"
                     /translation="MIPEPNDDRPEDAGRLRLPRSVARIRSSLAGRPPRQLLAAALFL
                     GTLTTIVHGSALRGNWRADDGDHLGFALAHEPWEYFFVPEVNRAHHGATITPWNVLFY
                     DVNLGLFGFEPSGHYLHMLLLVALAAWLLYAVLRKWLDGPASLVGASALVLAGPTVHI
                     AQFLMVGHYATGLVLCLVATYGWIRFLRGGPAAWLAAGAAAYLLATLCKETCVPLVLF
                     LPMLGEGSVRRRLAALVPFAGVALGYVLWRRAVLGVFVGGYSPTGEGFRVLESLRQLA
                     EVPRLLLGGGPAGAVGVGLLLAAFLLAWRDGRIRWSSTAGLLVAALLPVVPLTILPGI
                     RAADRFLFFPAAALAVGIAVLWPRRGDPPWRLVPALGVLVLLGVGHVRQAHSIRGELR
                     RMDALYDFALRADPQTEGLFVESDHGYFREVLSKARAARDRYDGKPPGRGLVVLSDTA
                     AALPALAGNEWSGRTYYRYANEAMRPMTATQLATAVAAQREALERGRLRELRVELKRE
                     SGILSWEFGPWNGEYQVNLPNGGLRLVRKGAFPWPDDERLDFIVCFEGERTWVACTPR
                     LGLPPGQRTLAWARPGLEPEPQASPSAR"
     gene            complement(4653..5174)
                     /locus_tag="PLP50_16425"
                     /pseudo
     CDS             complement(4653..5174)
                     /locus_tag="PLP50_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
BASE COUNT          667 a         1831 c         1878 g          798 t
ORIGIN      
        1 tgcacaagcc gctccaccgc ctgaaccggg tgatgctcga cgaggcgatg gaggccatcc
       61 tcgacgtcga cctcgtcctc ctcgtcatcg acgccgccga agcggaaggc gcgggcgacc
      121 gccacgccct cgagctcgtc ggaaaggcga agcctccccg gatcgccgtc ctcaacaagg
      181 tcgacctcgt ctccaagccg aagctcctcc cccggatggc ggcgctcgcc gcgacggggc
      241 tgttcgacga gatcgtcccg atctccgcgg cgaccggcga cggcctggag gggcttcccg
      301 agctcctcct ccggtacctg ccggaagggg acgacctcta tccggcagga acggtcacga
      361 cctccgacga gcggacccgc gtggccgagc tgatccggga gaagttcctc gagcgaacgc
      421 gcgaggagat tccctacggc ctcggcgtcg tcgtcgatga gtgggtcgtc gacgagacga
      481 aggggatgac gcgcgtgacg gcgacgctcg tcgtcgacaa ggagaaccac aagcggatcg
      541 tcatcgggct cggcggccag ctgatccgcg acgccgggac ggcggcgcgt ctcgagatcg
      601 agaagacgtt cggccggagg ttcttcctcg acctgcacgt cgtcgcccgg ccgggctggc
      661 gggaagaccc gcgcttcctc ggaaccctct ccagctgacg accgtaccct tgacgtacgc
      721 cgcggcgggc ccacctttcc aggagggccc ggagcgtccg gggaggtgtc gatgcagccg
      781 gagaggcgca ggagccaccg gatgagggtc gacggcgagg ggccgagcgg ggtcgtcaga
      841 ggcctcgcgc cggtctccgt gagcgacctc tccccgggtg ggcttcggct ccgtctcgac
      901 cgggcgctcg aggccggggc ggtctacccc ctcacgctct tcctgcgcgg gctcgccgtc
      961 accacggcgg tccgtgtcac gcgctgccgg cgggattcgc ggtcggcctg ggaggccgga
     1021 ggcgagttcc tctttcggga cccgacggac gccgcggcgg tccggcgctg gatcgacgcc
     1081 aggtcgaccc cggtgccctg acttgacggc gagcagcgaa cgccggatcg ttcccggtgc
     1141 ggaggattcc gtgaagccgg agagaagacg atcgcctcgg gtccgggccc cggaaggggc
     1201 tgagggcatc atccgctcga cgattcaggt ccaggtggag gacgtctcga aggacggggt
     1261 ctgttttcgg ctttccgggc ccgtccgacc gggctcgacc tacgccttcc acgcgagcct
     1321 cgaaggcctc gatctcgccg ccccgatccg aatcacgcgc tgcaaggcga tttcgcctgc
     1381 gatgggcggg ggccgagggc tcgtctacga ggcgggagcc gagttcgcct gggagagacc
     1441 gggcgacgag gagcgcgtcg tgacgtggct cgcgcgccga ggatcggccg gcggactgcg
     1501 ggccgagctc gaaggctgag ctcacgcccg ggcgctccgc ccgggtcctc gggtcacggg
     1561 ccgaggggaa cctccgcttc cggccgttcg accggcgggg cgccgggcgg ccccggtagc
     1621 tccgtgcgaa actcgccggc cgatgattcc cgagccgaac gacgaccggc ccgaggatgc
     1681 aggacgtctt cgcctccctc ggtccgtcgc ccggatccgg tcctccctcg ccggacgtcc
     1741 gccgagacag ctcctcgcgg cggccctgtt cctcgggacc ctgacgacga tcgttcacgg
     1801 cagcgcgctc cgtgggaact ggcgggccga cgacggcgac cacctcgggt tcgcgctcgc
     1861 ccacgagccc tgggagtact tcttcgtccc ggaggtcaac cgggcccacc acggggccac
     1921 gatcacgccc tggaacgtgc tgttctacga cgtcaacctc ggcctgttcg gcttcgagcc
     1981 cagcggccac tacctgcaca tgctgcttct cgtcgctctc gcggcctggc ttctgtacgc
     2041 cgttctgcgg aagtggctgg acggaccggc gtctctggtc ggcgcgtcgg cgctcgtcct
     2101 cgcggggccc acggtccaca tcgcgcagtt cctcatggtc gggcactacg cgacgggtct
     2161 cgtcctctgc ctggtggcga cgtacggctg gattcgcttc ctgcggggcg gccccgcggc
     2221 ctggctcgcc gccggagcgg ccgcctacct gctcgcgacg ctctgcaagg agacctgcgt
     2281 cccgctggtc ctcttcctcc cgatgctggg ggaggggagc gtccgccggc gcctcgcggc
     2341 gctcgttccc ttcgccgggg tcgcgctcgg ctacgtcctc tggcgccgag cggtcctggg
     2401 cgtcttcgtc ggaggctact cgcccacggg cgagggcttc cgcgttctcg agagcctccg
     2461 tcagctcgcg gaggtccccc ggctcctcct cggaggcggc ccggccggcg cagtgggcgt
     2521 cggcctcctc ctggcggcgt tcctgctcgc gtggcgggat gggcgaatcc gctggtcatc
     2581 gacggcaggc ctcctggtcg cagccctcct gccggtcgtt ccgctcacga tcctgcccgg
     2641 tatccgggcc gcggatcgct tcctcttctt cccggccgcc gccctcgcgg tcggaatcgc
     2701 cgtcctctgg ccgaggcggg gcgatcctcc ctggcggctc gtcccggcac tcggcgtgct
     2761 cgtcctcctg ggagtcggac acgtacgcca ggcccattcg attcgaggcg agctccggcg
     2821 aatggacgcg ctctacgact tcgcccttcg cgccgacccg cagacggaag ggctcttcgt
     2881 cgaatcggat cacggctact tcagggaggt cctctcgaaa gccagggcgg cccgggaccg
     2941 ctacgacggc aagccgccgg ggcgcggcct cgtcgtcctg agcgacaccg ccgcggccct
     3001 gcccgcgctg gccgggaacg agtggtccgg ccggacctac tacaggtacg cgaacgaagc
     3061 gatgcgcccg atgacggcga cccagctcgc gaccgcggtt gcggctcagc gggaagcgct
     3121 cgagcggggt cgactgcgag agctccgggt ggagctgaag cgcgaatccg gaatcctgag
     3181 ctgggagttc gggccgtgga acggcgagta ccaggtcaac cttccgaacg gcggcctccg
     3241 cctcgtccga aagggtgcct tcccctggcc cgacgacgaa cgcctcgatt tcatcgtctg
     3301 cttcgagggc gaacggacgt gggtcgcctg cactccgcga ctcggcctgc cgccgggcca
     3361 acgtaccctc gcctgggcga ggccgggcct cgagcctgag ccgcaggcga gcccttcggc
     3421 gcgctgatcg gaggacctcc ggggccgtcg gtcacgtctc gcgcgtcgaa cgacgggcct
     3481 cttcgcgtct ccgggcggga cgggcctcgt cgggagctcg tcgattccgt cgacacgacc
     3541 ggtgcccccc ggcttcactc ctttccggag ggctcgagct cttcgagtgc ccggcgcagg
     3601 aatctgccga gtcttcggct ctggccgctc gcctcgtcga gcttcgattc gacgaggcgc
     3661 ttcacgtcgg cggcctcgcg cttcacctcg tcgagacggt ccgaggactc gaggcgcgcg
     3721 acggcgtcct cgaggacgcc gaggcgtccc tcgagcgcgg tccgaagctc ctcgacgcgg
     3781 ccggaaaggg ccgattccag cgtggccagg cgctgctcga gcgcggcgag aacctcgtcg
     3841 agcctgcgct cgatcggcgc ggggagccgg tcgaggcgct cttcgagcgc ggctctcccc
     3901 tcgcgggcga cggtctcgag ccggtcggtc tcctcggagc gggcgcgtgc caggtcgtcc
     3961 aggcggctcg agagcgccgt gaacgcctcg gcccagacga tcgaggccgg gggcggctcg
     4021 ggcgcgggag gcgggggcgg tgccgggggg ggctcgaggg cgggagtcgc gtcggcggcc
     4081 ggtgcgggct cggcccgagt ctccggttcc gtgggcggaa cggcggccgg cgagcccgac
     4141 ttgatctccg cgtccgggac cgtcgacgcc gcaggctccg gctcgggggc cgagagctcc
     4201 ggcccgggaa cgggcggcag gcccccctcc tcgccgtcga cgggcgagtc gtcgaacgac
     4261 acgggttctg gcacggcggc gggggccgct tcgattcccg cggtctccgc gatggacgga
     4321 tcctccctgg cggtgtcgtc ggcgggatcg gagtccgccg gcgcggcgga ctcgggcggg
     4381 ggaggcggcg gagccagctg aggcgggacg gtggcgtcgc cgaagatctc gccgcgcggc
     4441 atcgtcggcc acgacgccgt cgtctcgaag ggcgattcgg tcggaggtgc gggcgccggt
     4501 cccaccgtgc tcgtcagggc gaggagctcg gccccgactg cggggatccg cgccgtccgc
     4561 gacgtgtcct ccgcggtggg ggcgacccac tccgggccca gcttctcccc gtcgggggag
     4621 cgctggaggc gcttgaggag ctggacgaag gtcatcttcg agacgagctt gaaagtctcg
     4681 acgacgccct tcccgccggc ggcgaccgcc tcgacgtacg ggaactcctg gaatccgagc
     4741 gactcctgca gcgcctggac cgaaaggact cccggaaggt cccgcttgtt gtactggatg
     4801 atgacggccg tctcctcgaa cgcctgcccg agctccgcca tgttctcgcg gaggttctgg
     4861 aacgactcga ggttgtgcga gagcatcgac cactgcgagt cgacgacgaa gacgatgccg
     4921 tcgacgccct tcaggacgag cttgcgggtc tcgttgtaga agacctgccc gggcaccgtg
     4981 cagagctgga agcggaccgt gtagcccttc acgtttccga gctcgaccgg gaggaggtcg
     5041 aagtagatcg tccggtcctg ggccgtcgag agagacagga gccggcccgt cgtcccctgc
     5101 tcgagcttct cgtggaggag gcggaggttc gtcgtcttcc cgcacaggcc ggggccgtag
     5161 tagacgatct tcgc
//
LOCUS       DAOONL010000193         5145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19433_length_5145_cov_10.062868, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000193 DAOONL010000000
VERSION     DAOONL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19433_length_5145_cov_10.062868"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLP50_16430"
     CDS             <1..203
                     /locus_tag="PLP50_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53179.1"
                     /translation="DAKLNIIAVGQVVLVDGSGNPVLDGSGNPTVGITKVLEEQLDTN
                     PTGSSAPAMKGGVSGGTSAGQK"
     gene            282..4415
                     /locus_tag="PLP50_16435"
     CDS             282..4415
                     /locus_tag="PLP50_16435"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HPA53180.1"
                     /translation="MRQSPIPRRALALPLAVAMALAAASPSSAQTVPTEGYDPLRQIT
                     AQLVKANLLLVQDVSGSMVWDKYGNNVGNDAHGRLSWESTLSNVRSTRTSPSNGRLQF
                     VGRQSNGTVRTVEDTDFRGAGNPGVYTWAYSSTGAPTYACNRNVDGCPNTTTDLTATQ
                     FPVLLFNSSQSRWEPAYRCRPTGSWRRSDLELPWSPGTYVYESKNFDFDECTLWSSGL
                     YFTYPSRMMNVKNALGNSVYIADDYSPPNPGLTGKEKWKDVVWASGLGTHVAKENTAA
                     GEVLSVDEAYRSMKGNTKSWRFNINTGFDLATVGVYNPAPTGTVTRNLAVKVNWVEAP
                     TDLFPAYHESAPASYLDSSGVPVRSPGLTSGGSPIAAGSWAIGWVTDPARYGTMSVRA
                     PGDVIGRNAERINFGLSTYSGEDDASYPFLFPIDTRDTNNVERLQGYLAARDAPDGTD
                     VYTYVPKQKYPILTDAANMERVRIGLSGSDATPTRNALQFAKKILDVTFSGGTIAAEG
                     KTWTLPADPKKDCLRTYGVVLVTDGMSNNGNPGDDNWLSPCGTVAGRNDCDGGDSGDD
                     CPRNWTEFAAQAANDIYTPGLTQTGGTKINPRVWAIGISSAVGPCELDFIAYMGRTDA
                     SSPSGDAGFGGYHSVRNPRLPNPAVAVKPNPVNGNAGTYDGPTGNRYWSKDTSKDYRA
                     PSDEPQVWWPTPPPAVTGTGHNAFFANDAAELGEAFATIVNATATGDYATNAPVSGMS
                     AAASNMVFLPSTSFPNWNGHLYAFDTNKEVTDPLYLAWDAGDNLNAMPSASRKIFTWN
                     PADNALVEIKVDNLGEAPLNAVPGLDAQVIDFIRGNDGSGSPRAWRLGPMINSTPAIV
                     GAPAPWVQNKVVDHKPFQIAAGGRDALLWVGSNDGMLHAFRATDGIEQIAILPPSLLS
                     TQVTLFNNYVADPGFSSGQPPDILNHIYGVSNSLRFGDVYFASEGTYKTVGIVTLGGG
                     GSDVFAVDVTSIPRPEDPDYATKPNISVLWKKSSGASSTDGVNGTLAQLGQAFSIPAF
                     APASSNTWRLIGGSGFRPENTVSAQKSNTNFVDPKVYVLDPMDGRVVQTKTLPALNKG
                     VPTAPQPFVGSQAFADSVLFDPKAKGWQDDNVAILGLQADLNGRIWFVHSSSSGSLDF
                     DQATVGIDVSTDIAPSQSQPIYFNPAASGYGDGVTAGCVAYAFGSGALYERSASITGS
                     GVGTHPAFVPRLYIATGQKSSFSGKLPGANVSAKPIAGVWTVENEDGTTSSVTFGKRT
                     QLTGPPFMLVPKSGKGTSTALFLLYDPDVGCNGFSYVAVAEFEGDSSCRPGTPTWKAF
                     PAGVGAASGFTIAGDKVLVSKSGIGEGERAGLYVPPNISAAIGGVAVPKVRWWKELK"
     gene            4512..>5145
                     /locus_tag="PLP50_16440"
     CDS             4512..>5145
                     /locus_tag="PLP50_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53181.1"
                     /translation="MVLEMMKKGFRADSALVAQLRAHQWSGDAQKKELAEKFAALQGL
                     DLEEIAWTAADPTPELRSAGLALLRRRGVDTAALDAVVAMGRTRSEAARRGVQRFLRE
                     LAQGTDLGAFLSQLAEREDTFAKLTALEMAHELPPDRAFTICRRVMVGTTDPVLRARA
                     LRAVAESAEIAATPPARALALGALKDDDEETRVQALAILERTPDEALVTHL"
BASE COUNT          918 a         1817 c         1660 g          750 t
ORIGIN      
        1 ccgacgcgaa gctgaacatc atcgccgtcg gccaggtcgt cctcgtcgac ggctccggca
       61 acccggtcct ggacggatcc gggaacccga ccgtgggcat cacgaaagtc ctcgaagagc
      121 agctcgacac gaacccgacc ggctcgtcgg ccccggccat gaagggggga gtctccgggg
      181 ggacgagcgc ggggcagaaa tagcaggcgg ggctccgccc ctcctgggcg tcatcagtac
      241 cggcagacga ttcatacgga agacacgagg agaccatggc catgaggcaa tccccaattc
      301 cccgccgcgc cctcgcgctg ccgcttgcgg tagccatggc gctcgccgca gcctcgcctt
      361 cttctgccca gacggtgccg accgaaggct acgacccgct gaggcagatc accgcgcagc
      421 tcgtcaaggc gaacctcctg ctcgtgcagg acgtctccgg atcgatggtg tgggacaagt
      481 acggcaacaa cgtcggcaac gacgcccacg ggcggctctc atgggagtcg acgctgtcca
      541 acgtccgttc gacgagaaca tcgccgtcga atggccgcct ccagttcgtc ggccggcagt
      601 cgaacggcac agtccggacg gtcgaggaca cggatttccg gggagcaggg aaccccggcg
      661 tctacacctg ggcgtactcc tccaccggcg cgccgaccta tgcctgcaac aggaacgtcg
      721 acggatgccc caacacgacg acggacctga cggcgaccca gttcccggtc ctcctcttca
      781 actcctctca gagccgctgg gagcccgcct accgctgcag accgaccggc tcgtggaggc
      841 ggagcgatct cgagcttccg tggtcgcccg gcacatacgt ctatgagagc aaaaatttcg
      901 acttcgacga gtgcaccctc tggtccagcg gactctactt cacctacccg tcgcggatga
      961 tgaacgtaaa gaacgccctc gggaactccg tgtacatcgc cgacgactac tcgccgccca
     1021 accccggcct cacgggcaag gaaaagtgga aggacgtcgt ctgggcctct ggcctgggca
     1081 cgcacgtggc gaaggagaac accgccgcgg gtgaggtcct cagcgtggac gaggcctacc
     1141 gcagcatgaa gggcaacacg aagtcctggc ggttcaacat caacacgggc ttcgacctgg
     1201 ccacggtcgg cgtctacaac ccggctccga ccggaaccgt cacgaggaac ctcgccgtga
     1261 aggtgaactg ggtcgaagct ccgacggacc tcttccccgc gtatcacgag tccgcaccgg
     1321 cttcgtacct cgattcctcc ggcgtccccg tccgttcccc cggcctgacc tccgggggca
     1381 gcccgatcgc ggccggcagc tgggcaatcg gctgggtcac cgatcccgcg cgttacggca
     1441 cgatgtcggt tcgggctccg ggagacgtca tcggccggaa cgccgagcgg atcaacttcg
     1501 gcctctcgac gtacagcggc gaggacgacg cgagctatcc cttcctcttt ccgatcgaca
     1561 ccagggacac gaataacgtc gagcgactcc agggctacct ggccgcgcgg gacgccccgg
     1621 acgggaccga cgtctacacc tatgttccga agcagaagta cccgatcctg accgatgccg
     1681 cgaacatgga acgcgttcgg atcggcctct ccggaagcga cgcgacgccg acgaggaacg
     1741 cgctgcagtt cgcgaagaag atcctcgatg tgacgttcag cgggggaacc atcgctgccg
     1801 aaggcaagac ctggacgctt cccgccgatc cgaagaagga ctgcctgcgc acctacggcg
     1861 tggtcctcgt caccgacggc atgtcgaaca acgggaaccc cggagacgac aactggctca
     1921 gtccgtgcgg tacggtcgcc gggcggaacg actgcgacgg cggagattcg ggcgacgact
     1981 gcccgaggaa ctggaccgag ttcgccgccc aggccgccaa cgacatctac acgccgggac
     2041 tgactcagac gggcgggacc aagatcaacc cgcgcgtctg ggccatcgga atcagctcgg
     2101 ccgtcggccc ctgcgagctc gatttcatcg cctacatggg gcgcaccgac gccagcagcc
     2161 cgagcggcga cgcgggcttc ggcggatacc attccgtgag aaacccgcgg cttccgaatc
     2221 ccgccgtggc cgtgaagccc aatccggtca acggaaacgc gggcacctac gatggcccga
     2281 cggggaaccg gtactggtcg aaggacacgt cgaaggatta ccgggctccg agcgacgaac
     2341 cgcaggtctg gtggccgacg ccccccccgg ccgtgacggg caccggccac aacgcgttct
     2401 tcgcgaacga cgccgccgag ctcggcgagg ccttcgcgac gatcgtgaac gcgacggcca
     2461 cgggcgacta cgccacgaat gcgcccgtct ccggcatgtc cgccgcggca tcgaacatgg
     2521 tcttccttcc ctccacctcg ttcccgaact ggaacggcca tctctacgcg ttcgacacga
     2581 acaaggaggt caccgacccc ctgtacctcg cctgggacgc cggggacaac ctcaacgcga
     2641 tgccgtctgc ctcccggaag atcttcacgt ggaaccccgc cgacaacgcc ctcgtcgaga
     2701 tcaaggtcga caacctcggc gaggcccccc tgaacgcggt ccccgggctc gacgcccagg
     2761 tcatcgactt catccggggg aacgacggat cggggagccc gagggcgtgg cggctcggac
     2821 cgatgatcaa ctccacgccc gcgatcgtgg gcgctcccgc cccctgggtc cagaacaagg
     2881 tcgtggacca caaaccgttc cagatcgcgg cgggcggccg ggacgcgctg ctctgggtgg
     2941 gctcgaacga cggcatgctc cacgccttcc gggccacgga cgggatcgag cagatcgcga
     3001 tcctgccccc gagcctcctc tcgacacagg tcactctctt caacaactac gtcgccgatc
     3061 ccggtttctc cagcgggcag ccgcccgata tcctgaacca catctacggg gtctcgaact
     3121 ccctccggtt cggtgacgtc tacttcgcca gcgaggggac ctacaagacc gtcggcatcg
     3181 tcacgctcgg cggcggcggg agcgacgtct tcgccgtcga cgtgacgtcg attccccggc
     3241 cggaggaccc ggactacgcg acgaagccga acatctccgt tctctggaag aagtcgagcg
     3301 gagcctcgag cacggacgga gtgaacggca cgctggcaca gctcgggcag gctttctcca
     3361 ttccggcgtt tgcgccggcc tcgtcgaaca cgtggcgact catcggagga agcggcttcc
     3421 gcccggagaa caccgtctcc gcacagaaga gcaacaccaa tttcgtagac ccgaaggtct
     3481 acgtcctcga cccgatggat gggcgggtcg tccagacgaa gaccctgccc gcgctcaaca
     3541 agggagtccc cacggcccct cagcccttcg tcggatcaca ggcctttgcc gactcggttc
     3601 tcttcgaccc gaaggcgaag ggctggcagg acgacaacgt cgcgattctc ggactccagg
     3661 ccgacctgaa cgggcggatc tggttcgtcc actcctcgag cagcggcagc ctggacttcg
     3721 accaggcgac cgtcgggatc gacgtctcga cggacatcgc tccgtcccag tcgcagccca
     3781 tctacttcaa cccggcggcc agcggctacg gagacggcgt caccgccggc tgtgtcgcct
     3841 acgcgttcgg cagcggagcg ctctacgaga ggagcgccag catcaccggc agcggtgtcg
     3901 gaacacatcc ggcattcgtg ccgcggctct atatcgcgac cggccagaag agctccttct
     3961 ccggaaagct ccctggcgcg aacgtctcgg ccaagccgat cgccggcgtc tggaccgtcg
     4021 agaacgagga cggaaccacg agctccgtca cgttcgggaa gcggacgcag ctcacgggac
     4081 ctccgttcat gctcgttccg aagtcgggca agggcacgtc gacggcgctg ttcctcctct
     4141 acgatcccga cgtcggctgc aacggcttct cctacgtcgc ggtcgccgag ttcgagggtg
     4201 actcgagctg cagacccggc acgccgacct ggaaggcctt cccggccggg gtcggtgccg
     4261 ccagcggctt cacgatcgcc ggcgacaagg tgctggtctc caagagcgga atcggcgaag
     4321 gcgagcgggc cgggctctat gttcccccga acatctcggc cgccatcggc ggggtagcgg
     4381 ttccgaaggt ccggtggtgg aaggagctga agtagctcct ccctctcccg agcgtttccg
     4441 gaaaggggcg ggtcctcccg cccctttcct ttttccgctg ggctaggatt cccggagacg
     4501 gagggaacgc cgtggtcctc gagatgatga agaaggggtt ccgcgccgac tcggccctgg
     4561 tcgcgcagct gcgggcgcac cagtggagcg gcgacgccca gaagaaggag cttgcggaga
     4621 agttcgcggc gctgcagggg ctcgacctcg aggagatcgc ctggacggcg gccgacccga
     4681 cgcccgagct ccggtcggcc ggcctcgcgc tgctccggcg tcggggggtc gacacggccg
     4741 cgctcgacgc cgtcgtggcg atggggcgga cccgctccga ggcggcccgg cggggcgtcc
     4801 agcgcttcct ccgggagctc gcgcagggga ccgacctcgg cgcgttcctc tcgcagctcg
     4861 ccgagaggga ggacacgttc gcgaagctga cggcgctcga gatggcgcac gagctgccgc
     4921 ccgaccgcgc gttcaccatc tgccgccgcg tcatggtcgg cacgaccgac ccggtccttc
     4981 gggcgcgagc gctccgggcc gtggccgagt cggcggagat cgccgcgacg ccgccggcgc
     5041 gcgcgctcgc cctcggcgcg ctgaaggacg acgacgagga gacccgggtt caggcgctcg
     5101 cgatcctcga gcggacgccg gacgaggcgc tcgtcacgca cctcc
//
LOCUS       DAOONL010000194         5058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19898_length_5058_cov_15.402958, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000194 DAOONL010000000
VERSION     DAOONL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19898_length_5058_cov_15.402958"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..738
                     /locus_tag="PLP50_16445"
     CDS             577..738
                     /locus_tag="PLP50_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53182.1"
                     /translation="MSYLLLAAAQHLSGLEEQAARTIAEGRRNRRTNVKLLESVRWGA
                     VRSAPEPAP"
     gene            875..>1464
                     /locus_tag="PLP50_16450"
     CDS             875..>1464
                     /locus_tag="PLP50_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53183.1"
                     /translation="MKWTRAILFSAWAAAAAHAGNVQPLLRFGDADVSTSAAARVVFS
                     PMDAAGAADVVCAPADACTIPPGRYSIRLDSPDYVALDRPTFVSDSDASSVPTPFALR
                     VFPAARVKLGPGRFPEGSVLTVLDPESGLSFKAAVRSRETSVQVPARKVVAAVFRGAA
                     EVAELWRPVTLTAGQHLELPENPPLARGRGQAVVSLR"
     assembly_gap    1465..1564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1565..2874
                     /locus_tag="PLP50_16455"
     CDS             <1565..2874
                     /locus_tag="PLP50_16455"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA53184.1"
                     /translation="RPRLSAQFEPNDRLPAGEAQVDLLDCRRLKGVSGPPRFSLCTSA
                     SIRKGPVTGEFRFEGLDPVLHALRWKAGRFQDYFLVDLEAAESKDVKIPVRIHRVSGE
                     VTRRGRPVANARISWLHQVTGIESETVSDEEGRFEALLAPAGWYLTFLDGPGFKRHAD
                     ALDVSEDREADFVVPSNETEVRVVDTATGLPVEGARVGWHLEGKGDLQVARAESVDCD
                     EDGLARLPPFPAGRVTVLARARGYRPSEEKRFDVTKESATTTIELRIEKGDGTRLRIV
                     DAAGSPLQGAWAMLPSGIRSESADAAGEVFLESALAAGAPLFVTTPAGALALARFVAE
                     DVPVRVGPPSPPFTVRFLTADGRPVASEQIAYSIDGVRVPQPIDYLARLAAGGDLSSR
                     LDGTLRVAGLPAAGVVALWPARKPDAVVTRPLPVSELLELPAP"
     gene            3223..3615
                     /locus_tag="PLP50_16460"
     CDS             3223..3615
                     /locus_tag="PLP50_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA53185.1"
                     /translation="MICDAGLLAEDDALALVAHGLREVALRKAVSERTIRRRFDAAGT
                     PVHVVVHRVRIEVTRSALVADVPTTVIARWLGFTNPEAYRRFVRRETGVSIREIRTRV
                     RESAELAAKRGCAERSLSLPSGERFNSE"
     gene            4438..>5058
                     /locus_tag="PLP50_16465"
     CDS             4438..>5058
                     /locus_tag="PLP50_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53186.1"
                     /translation="MAALMGASHAGVAGEVAPPGPLRFVDRFREIVKGVSLCVEEGDS
                     CRPVATATEGAIDLPLDLRKQKVVATAEGFRPRVVLPHEQEVALIGTGVISVRLELMS
                     RDETDVDLLLIRQGSTPGIRESRHLAARAPRTVRFSDLPPGSYRLTATGPQLVEETKT
                     IGLAPSRQVDIGSLFVRRGRLISGMILEAAGLPISGAVVELLPEDGG"
BASE COUNT          734 a         1704 c         1673 g          847 t
ORIGIN      
        1 ccgaccgggc ggcttcgcgc atctgccgct cggcgcctat cagctgccgc ttccctcggg
       61 ggccgagctc cggagcccgc tgtcggaggt gccgtgaagc tgcctctccg tccggcgatc
      121 tgcgtcgtcc tcctcgccgc ggctttccgg gggggcgcga gtcctgccga tggcaagccg
      181 ggatcggggc tcggacctgt gagccgggcg gagaggcccc cgtccgacga tgaaagcgag
      241 gcgtcacgcc ttcgcgaggc tgctcggatc gaaggcctcg gggggccggc caccgtaact
      301 ctggcgagat ggctcctgtc tcactcacgt ttcgacgagg cgttgccgtt cctgagccat
      361 tcgtgtggag ctcgaagaga cgagtggagc tgcgccgccg cggccaccgt ccacgtggcg
      421 cgcggggagt gggtgccggc acggcgcatt gcagccgagt atgtcgaacg cgcctgcttc
      481 gaaagcgatc gtttgaggac ggtgactctg ctcgaatcgc ctgcattggc cgtcacggcc
      541 ggggaagggc tcgtccggct cgctccaacg gatcagatgt cctacctgct gctggcggcg
      601 gcccagcacc tctccgggct cgaggagcag gcggcccgga ccatcgcgga gggtcgacgg
      661 aaccggcgga cgaacgtgaa gcttctcgag agcgtccggt ggggggctgt acggagcgct
      721 cccgaaccgg cgccctgacc cgcagcgggg tcctgctccg gacgcccgct acgattgccc
      781 tgctccggct tctccggccc cgtcatccgc gccgaacccg accgcgccgg gacgggatct
      841 cgtcgccatg cgacactcga acgcgagatc ttccatgaaa tggacccggg cgatcctctt
      901 ctccgcgtgg gcggccgcgg ccgcccacgc gggcaacgtc cagccgctgc ttcgtttcgg
      961 cgacgccgac gtctccacgt cggccgccgc ccgcgtcgtc ttctctccga tggatgcggc
     1021 cggcgccgcg gacgtcgtct gcgcgccggc cgacgcttgc acgatccctc cgggccgcta
     1081 ctcgatccgg ctcgactcgc ccgactacgt cgcgctcgac cgtccgacgt tcgtctccga
     1141 ttccgacgcg tcgagcgtcc cgactccctt cgcgctccgg gtcttcccgg cggcccgcgt
     1201 gaagctcggc ccgggacgct tcccggaggg gagcgtcctg accgtcctcg atcccgagtc
     1261 cggcctcagc ttcaaggccg ccgtccgctc tcgggagacg tccgtgcaag ttcccgcgcg
     1321 gaaggtcgtg gcggcggtct ttcgcggcgc ggccgaggtg gccgagctgt ggcggcccgt
     1381 caccctgacc gcggggcagc acctcgagct tccggagaac ccgcccctcg cgagagggag
     1441 ggggcaggcc gtcgtgagcc tccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnctcgtc cccgcctgag cgcgcaattc gagccgaacg accgactccc cgccggcgag
     1621 gcgcaggtcg acctgctcga ctgccgccgc ctgaagggcg tgagcggccc gccccgcttc
     1681 tcgctctgca cgtcggcttc catccggaag ggccccgtca cgggagagtt ccgttttgaa
     1741 ggcctcgacc ccgtgttgca cgcgctgcgc tggaaggcag gccggttcca ggactacttc
     1801 ctcgtcgatc tcgaagcggc cgagtccaaa gacgtgaaga tccccgtgag gattcaccgc
     1861 gtgagcgggg aggtcacccg gcggggtcgt cctgtggcca atgcccggat ctcctggctg
     1921 catcaggtga cgggcatcga atccgagacc gtttcggacg aggagggccg cttcgaggcc
     1981 ctcctcgctc ccgccggctg gtacctcacg ttcctcgacg gcccgggctt caagcgacac
     2041 gcggacgctc tggacgtgtc cgaagaccgc gaagcggact tcgtcgttcc ttcgaacgag
     2101 accgaggtcc gggtcgtcga tacggcgacc ggcctgcccg tcgagggtgc tcgtgtcgga
     2161 tggcacctcg agggaaaggg cgacctgcag gtcgcgcgcg ccgagagcgt ggactgcgac
     2221 gaggacgggc ttgcccggct ccccccgttc ccggccgggc gcgtcaccgt tctggcgcgg
     2281 gcgcgcggct atcggccttc cgaggagaag cggttcgacg tcacgaaaga gagcgccacg
     2341 acgacgatcg agctccggat cgagaagggg gacggaacgc ggctcaggat cgtcgatgcc
     2401 gccggcagcc cgctccaggg agcctgggcg atgctcccct ccggaatccg ctcggagagc
     2461 gcggacgccg cgggcgaggt gttcctcgaa tcggcgctcg cggccggggc gccgctcttc
     2521 gtcacgacgc cggccggcgc cctcgccctg gcccggttcg tggccgagga cgttcccgta
     2581 cgggtcgggc cgccttctcc ccccttcacc gtccggttcc tgacggcgga cgggcgcccg
     2641 gtggcttccg agcagatcgc ctactccatc gacggcgttc gggtccccca gccgatcgac
     2701 tacctcgccc ggctcgccgc cggaggcgac ctcagctccc ggctcgacgg aacgctcaga
     2761 gtggccggcc tccccgccgc cggcgtcgtc gccctctggc cggcccgcaa gcccgacgcc
     2821 gtcgtcacac gcccgctgcc cgtctccgag cttctcgagc tcccggcgcc ctgagcgaaa
     2881 tcgaggcgcg gccctgctgc agccgttctc gcgagtgacg ccccggagcg gatgcacggc
     2941 gtctcctctc gggccgactc tctcgatcgg acggtgacgc ggttcgtcga actcgtgtcc
     3001 gcaagaatcg cgccaagaat atttcgtcgg caggtgcact ggcacccctc tccacccctc
     3061 atacaaggtg gagggactct cgtgcgtcaa cgagatcaac ttgctgaaac acagtcgcag
     3121 tgccgccggc ctcgtcgtct gtgcgacagc ccgacccgct cccttcccaa cccgaagttc
     3181 cggcatcccg gatacgacga gctccgcgcc gtcgtccgag cgttgatctg cgacgcgggc
     3241 ctcctcgcgg aggacgacgc gctcgctctc gtcgcccacg gacttcgaga ggtcgcgctg
     3301 cgaaaggcgg tctcggagcg gacgatccga cgcaggttcg acgcggccgg aacgccggtc
     3361 cacgtcgtcg tccatcgcgt ccggatcgaa gtgacccggt ccgcactggt cgccgacgtc
     3421 ccgaccacgg tgatcgcgcg ctggctcggc ttcacgaacc cggaggcata ccgaaggttc
     3481 gtgaggcgcg agacgggcgt gtcgatccgg gagatcagga cccgtgtccg cgagagtgcg
     3541 gaacttgctg ccaagcgggg ttgtgctgaa cgctcattgt cgctaccgtc tggcgagcgc
     3601 ttcaactcgg agtaaactag tgtcgttccc ccggaccctc ccaccggcac attgtgcaga
     3661 acaaaaatgg aggttggact tcgtgaatca tttcagagga atggaactcg cgtccgtcct
     3721 atcgatggtg ctagtcgcct cgcccgttgt agcagcggat gctcagagct cgcgcagcgg
     3781 tccggtcgag gctcaggcag gccagacctt cgtcggcgcg acgaacaccg gtctcgagat
     3841 ctctgtcaag ccgcccatcg gcggtccaac gacttatgtg ttccacacgc caagtgctcc
     3901 agagtcagca gtccccagtc ttcgcgagaa cgccgaggtg ctggcctcga gcgacagcgt
     3961 cgttgccatc ttccatgaga ccgctgaggc ggtactggtt cgtgtcgatg gcgctgctgc
     4021 cgaccttgga gcgtttgtac ctactggcta ccggctcacg acgtttgagg gtgggtcgct
     4081 gatcacaaag aagcctgcgg cccggctgaa gaagttggga gtaggcaacg ttgcggacac
     4141 agaggcacca aacgccttca accaggatcc gggaccggga gatgacgggg gggggcccgg
     4201 gtgcgcgaag agctgctcca agacctgtgg gaagggcccg acggcgacgt cgtgctcctg
     4261 cacggcgggg gacgggttct gttgcgagtg cacttgcagt gcgacctccg gggcgacctg
     4321 tggcacatcg cggggatgag ccgagggact gaccttcact catgactacg gcggaatccc
     4381 ttcggtgagg tttcccgtat gagaggagta tcttgcgcga aggccgtctt gtgcgcggtg
     4441 gcggcactga tgggagcgtc gcatgcgggg gttgccggcg aggttgctcc ccccgggcct
     4501 ctccggttcg tcgacaggtt tcgcgaaata gtcaagggag tctctctctg tgttgaggaa
     4561 ggcgactcct gtcggcctgt cgcaacagcg accgaggggg ccatagactt gcccctagac
     4621 ctgcgcaagc aaaaggtggt cgcgacggct gagggcttcc gcccacgggt cgttcttccg
     4681 cacgaacagg aggtcgcact gatcgggacg ggagtgatct cagttcgctt ggagctgatg
     4741 tcccgtgacg agactgacgt cgatctcctt ctgatacgac aaggaagtac gccgggcatc
     4801 cgagagtccc gccatcttgc tgcccgcgca ccccgcaccg ttcgcttttc tgatttgccg
     4861 cctggctcgt acaggctgac ggctacaggt ccgcagcttg tagaagagac taagaccatt
     4921 ggcctggccc cctcacggca agtcgatatt ggctcccttt tcgtccgccg cggccgtctg
     4981 atcagtggaa tgattctcga ggccgctgga ttgcctatca gcggggccgt cgtcgaactc
     5041 ctgccggagg acggaggg
//
LOCUS       DAOONL010000195         5041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_19996_length_5041_cov_16.652627, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000195 DAOONL010000000
VERSION     DAOONL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19996_length_5041_cov_16.652627"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="PLP50_16470"
     CDS             complement(<1..1737)
                     /locus_tag="PLP50_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPA53187.1"
                     /translation="MRLVLALLLLLSPVAAGDDDPVFSARGVPSGRILAEAERDPSVP
                     LPATVLGHRIGDALTPHDALVRYVRALEAASPRVSAHAYGRTPEGRDLFRVAVSSPAN
                     LARLDAIAADLARLADPARTSKDEARAIAERSPAVAWIACGIHGNEVAGPEACAALLY
                     TLAASRSAEVEKLLSALVVVVDPCANPDGHERHVSWWRSVAGAEPDPDPRALENEAPW
                     PSGRGNHFGVDLNRDWAWATQPETRARIAALRALPPQLYVDVHEMGPEQSYFFPPPAE
                     PVHPRVPDSTRAWIEAFGAANAEAFDERGWGYFTREVFDLFYPAYGDSWPVFRGMVGM
                     TYEVGGKGGLAHRRRDGAVVTLKERALKHWTALWATLRTAAAGRARLLADYAEFFRAS
                     AAEGRRAFVVPGGQDPSRLSALASLLALQDVEVKVTTRDLPNVPGKGETLPSGSLVVE
                     TAQRFGRFAEVLLESQAALAPRFLEEERRRLLSEEDERFFDVTAWSLPIAFGLEARLL
                     PGIGPLAGSTTPWRPAEPASPRGGRFGWLLPGDDAASRRCAARLAASGVRVSVTTKEA
                     RAASGPVPAGSYL"
     gene            complement(1734..3122)
                     /locus_tag="PLP50_16475"
     CDS             complement(1734..3122)
                     /locus_tag="PLP50_16475"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA53188.1"
                     /translation="MKVLIVDDEPVVREVLRVVLSRAGYATSEAATAAEGLALFADPA
                     VDLVLLDLMLPDRPGLSLLPELRERRPDVPIVVVTAYSSVESAIAAMKEGAFHYVPKP
                     FRNEEVVHLVAQALEKRRLVAENLALRARLDSGSDFPEMVGRSAPMETVFQTIRQAAP
                     ARSTILVTGESGTGKELVARALHRLSQRAAGPFVTVHSGALPPDLLESNLFGHVRGAF
                     TGAVAEKKGLFKVADGGTIFFDEIGTVPLETQAKLLRVLQEREFLPVGAVEPVRSDVR
                     VVAATNADLAKLVEEGKFREDLYYRLCVISIGLPPLRERKEDIPLLVEALVSRAAREN
                     DRPVPPVTPAAMKALLDHDWPGNVRELENVLERAVVLGNGLVDLDQLPESVRRSIPRP
                     EAIPADGIAFYDAVTRYEKALLASAMQRAGGVQKEAARLLELSPTTLNEMLKRHGMIA
                     RRSPRAAEPEPA"
     gene            complement(3119..>5041)
                     /locus_tag="PLP50_16480"
     CDS             complement(3119..>5041)
                     /locus_tag="PLP50_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA53189.1"
                     /translation="EKQVRFLLAGTAGGLLPVVLLDFLPRLLGAPIPIVSSFSIVPLV
                     LVPVAFVAALTRYRLWDVEILTRETVALLGAAFLGAGLFSLAQVVDLGRVLPGIPYGR
                     GIVETGAGLLIALTFFPVRRGLSTALARVQYGERFGEREGLLALVRDLARPRRLAEIG
                     PLLAQRVKNGLGVPHATLLLALPDGRFDASALDGGDPLPAEELPPEAASRTLRLSRLA
                     FTERPTTAVARLRHAGFRTIAPLALSGRLLGLFAVADREGRDPLSAEDVELLETVLAS
                     AALAVDHARLYGELEEQAERYRRLKEFHEDVVTGSPAAIAATDDSGRITSANPAFEAL
                     FGPPSGELVGRRAEEVLPEAVLSARAPARVEVTVGREPRAIDVAVSPFPGASEGSAAR
                     VWVLSDATELARLERSLAEKERLSALSSLSAGVAHEVNTPLTGVASYARLLLDDTPAD
                     DPRRPLVEKIERQAFRAARLVGSLLDLARGRPRDLSPLAPRELVAEACRALEDEAKGR
                     RVRLDVDLPERLPNVLGHADALVQVLVNLLKNAVEAVAVPKEGRPGPGSVRIAAAPLS
                     GSVVFTVEDDGPGLSSAEQERVFAPFHTTKGGQGGVGLGLAIAGDIIRAHGGSLAVDS
                     APGRGARFTVSLPAQT"
BASE COUNT          720 a         1721 c         2016 g          584 t
ORIGIN      
        1 gaggtaggag ccggcgggaa cgggacccga ggccgcgcgg gcctccttcg tcgtcacgga
       61 gaccctcacg ccggaggcgg cgagcctcgc cgcgcaacgc cggctcgcgg cgtcgtcacc
      121 ggggaggagc cagccgaatc ggccccctct cggagaagcg ggctccgcgg gcctccaggg
      181 ggtcgtggag cccgcgagcg gcccgatgcc ggggagaagc cgggcctcga ggccgaaggc
      241 gatcgggagg ctccacgccg tcacgtcgaa gaaacgctcg tcctcctcgg acagcaggcg
      301 gcggcgctcc tcctcgagga aacgcggggc cagggcggcc tgcgactcga ggaggacctc
      361 ggcgaaccgg ccgaagcgct gcgccgtctc gacgacgagc gagcccgagg ggagcgtctc
      421 tcccttcccg gggacgttcg gcaggtcgcg ggtcgtcacc ttcacctcga cgtcctggag
      481 cgcgaggagg gaggccagtg ccgagagccg ggacggatcc tgaccgcccg ggacgacgaa
      541 ggcgcgacgc ccctccgcgg ccgaggcgcg gaagaactcc gcgtagtcgg cgaggaggcg
      601 ggcgcggccg gccgcggcgg tccggagcgt cgcccagagg gccgtccagt gcttcagggc
      661 gcgctccttc agcgtcacga cggcgccgtc gcgccggcgg tgggcgaggc cccccttccc
      721 gccgacctcg tacgtcatcc cgaccatccc ccggaacacg ggccaggagt cgccgtaggc
      781 ggggtagaag aggtcgaaga cctcgcgggt gaagtagccc cagcccctct cgtcgaacgc
      841 ctccgcgttc gcggcgccga aggcctcgat ccacgcccgc gtggaatcgg ggacccgcgg
      901 gtggaccggc tcggccggcg gggggaagaa atagctctgc tccgggccca tctcgtggac
      961 gtcgacgtag agctgcggcg gcagggcgcg gagcgcggcg atccgcgcgc gcgtctcggg
     1021 ctgcgtggcc caggcccagt cccggttcag gtcgacgccg aagtggttcc cgcggccgga
     1081 gggccagggg gcctcgttct cgagcgcgcg ggggtcgggg tcgggctcgg cgcccgcgac
     1141 ggagcgccac caggagacgt gccgctcgtg cccgtcgggg ttggcgcacg ggtcgacgac
     1201 gacgacgagg gccgagagga gcttctcgac ctcggccgat cgcgaggcgg cgagcgtgta
     1261 gaggagcgcg gcgcacgcct ccggaccggc gacctcgttg ccgtggatgc cgcaggcgat
     1321 ccaggcgacg gccgggctcc gctcggcgat ggcccgcgcc tcgtccttcg aggtccgcgc
     1381 cgggtcggcg aggcgggcga ggtccgcggc gatcgcgtcg agccgggcga ggttcgccgg
     1441 gctcgagacg gcgacgcgga agaggtcgcg cccttcggga gtccgcccgt aggcgtgcgc
     1501 cgagacgcgg ggagaggcag cctcgagggc ccggacgtac cggacgagcg cgtcgtgcgg
     1561 cgtgagcgcg tcgccgatcc ggtgcccgag gacggtggcc ggaaggggga ccgacgggtc
     1621 gcgttccgcc tccgcgagga tccgtcccga cgggactccc cgcgcggaga agaccggatc
     1681 gtcgtcgccc gcggcgacgg gcgagagcag gaggagcaac gcgaggacga gcctcacgcc
     1741 ggctcgggct ccgcggcgcg cggggagcgc cgggcgatca tcccgtggcg tttgagcatc
     1801 tcgttgagcg tcgtcgggga gagctcgagg aggcgggccg cctccttctg caccccgccg
     1861 gcgcgctgca tcgccgaggc gaggagcgcc ttctcgtagc gcgtgacggc gtcgtagaac
     1921 gcgatcccgt cggccgggat cgcctccggg cgcggaatcg aacgccggac gctctcgggg
     1981 agctggtcga ggtcgacgag gccgtttccg aggacgacgg cgcgctccag gacgttctcc
     2041 agctcgcgga cgttcccggg ccagtcgtgg tcgaggagcg ccttcatcgc ggcgggtgtg
     2101 acgggcggga cgggccgatc gttctcccgg gcggcgcgcg agacgagcgc ctcgacgagg
     2161 agcgggatgt cctccttccg ctcgcggagg ggcggaaggc cgatcgagat gacgcagagc
     2221 cggtagtaga ggtcctcccg gaacttcccc tcctcgacga gcttggcgag gtcggcgttc
     2281 gtcgcggcga cgacgcggac gtcggagcga accggctcga cggcgccgac ggggagaaac
     2341 tcgcgctcct ggaggacgcg gaggagcttg gcctgcgtct cgagcgggac ggtgccgatc
     2401 tcgtcgaaga agatcgtccc gccgtcggcg accttgaaga gccccttctt ctccgcgacg
     2461 gccccggtga aggccccgcg gacgtgcccg aagaggttcg actcgagcag gtcgggcggc
     2521 agggcgccgg agtggaccgt cacgaacggc cccgcggcgc gctgcgagag ccggtggagc
     2581 gcgcgggcga cgagctcctt gcccgtcccg ctctcgccgg tgacgaggat cgtcgagcgg
     2641 gcgggggccg cctgccggat cgtctggaag accgtctcca tcggcgcgga tcggccgacc
     2701 atctccggga agtcgctccc ggagtcgagg cgggcccgca gcgcgaggtt ctccgcgacg
     2761 agcctccgct tctcgagcgc ctgcgcgacg aggtggacga cctcctcgtt gcggaacggc
     2821 ttcgggacgt agtggaaggc cccctccttc atcgccgcga tcgcgctctc gaccgaggag
     2881 tacgcggtca cgacgacgat cgggacgtcc gggcgcctct cgcgaagctc gggaaggaga
     2941 gagaggccgg ggcggtccgg caacatcagg tcgaggagga cgaggtcgac cgccgggtcg
     3001 gcgaagaggg cgagcccctc ggcggccgtg gcggcctcgc tcgtggcgta cccggcccgc
     3061 gaaaggacca cccggaggac ctcgcggacg acgggctcgt cgtcgacgat caggactttc
     3121 atgtctgcgc ggggagactc accgtgaacc gggcgccccg gcccggagcg gaatcgaccg
     3181 cgagagagcc tccgtgagcg cggatgatat cccccgcgat cgcgaggccg agcccgacgc
     3241 ccccctgtcc tcccttcgtc gtgtggaagg gggcgaagac ccgctcctgc tccgcgctcg
     3301 agagccccgg cccgtcgtcc tcgacggtga agacgacgct tccggagagg ggggcggcgg
     3361 cgatccggac cgagcccggg ccggggcgcc cctccttcgg aacggcgacg gcctcgaccg
     3421 cgttcttcag gaggttcacc aggacctgca cgagcgcgtc ggcatggccg aggacgttcg
     3481 gcaggcgctc cggaaggtcg acgtcgaggc ggacgcggcg ccccttcgcc tcgtcctcga
     3541 gcgctcggca cgcttccgcg acgagttccc gcggcgcgag cggcgagaga tcacgcgggc
     3601 ggccgcgcgc caggtcgagg aggcttccga cgaggcgcgc ggcgcggaag gcctgacgct
     3661 cgatcttctc gacgaggggg cgccgcgggt cgtcggcggg cgtgtcgtcg aggaggagac
     3721 gggcgtagct cgcgaccccg gtgagcggcg tgttcacctc gtgcgcgacg ccggcggaga
     3781 ggctcgagag ggccgagagg cgctctttct cggcgaggct ccgctcgagg cgcgcgagct
     3841 cggtggcgtc ggagaggacc cagacccgcg ccgcggagcc ttccgaggcg cccgggaagg
     3901 gcgagacggc gacgtcgatc gcgcgcggct cccggccgac cgtcacctcg acgcgggcgg
     3961 gcgcccgggc cgagaggacg gcctcgggga ggacctcctc ggcccgtcgc ccgacgagct
     4021 ctccggaggg ggggccgaag agcgcctcga aagccgggtt cgccgaggtg atccggcccg
     4081 aatcgtcggt ggcggcgatc gccgcgggcg agccggtgac gacgtcctcg tggaactcct
     4141 tcagcctccg gtagcgctcc gcctgctcct cgagctcgcc gtagagtcgg gcgtggtcga
     4201 cggcgagagc cgcagaggcc aggacggttt cgagcagctc gacgtcctcg gccgacagcg
     4261 ggtcgcgtcc ttcgcggtcc gcgacggcga agaggccgag gagccggccg gagagggcga
     4321 gcggcgcgat cgttcggaaa ccggcgtgcc ggaggcgtgc caccgcggtc gtcgggcgct
     4381 ccgtgaacgc gaggcgcgaa aggcgcagcg tccgcgacgc cgcctcggga gggagctcct
     4441 ccgcgggaag cggatcgccg ccgtcgagcg cggaggcgtc gaagcggccg tcgggaaggg
     4501 cgaggaggag cgtggcgtgc ggcacgccga ggccgttctt cacgcgctgg gcgaggaggg
     4561 gcccgatctc ggcgagacgc ctcggccggg cgaggtcgcg cacgagggcg aggaggccct
     4621 cacgctcgcc gaaccgctcg ccgtactgga cgcgggcgag cgcggtcgaa agtccgcggc
     4681 gcacgggaaa gaacgtgagc gcgatcagga gcccggctcc cgtctcgacg atcccgcgcc
     4741 cgtaggggat ccccgggagg acccggccga ggtcgaccac ctgcgcgagc gagaagaggc
     4801 ccgcgccgag gaacgccgcc ccgagcaggg ccaccgtctc gcgcgtgagg atctcgacgt
     4861 cccagaggcg gtagcgcgtc agggccgcga cgaacgcgac gggaacgagg acgagcggca
     4921 cgatggagaa gctcgagacg atcgggatcg gcgcgccgag gagccggggg aggaaatcga
     4981 ggaggaccac gggcaggagg cccccggccg tcccggcgag gaggaagcgg acctgcttct
     5041 c
//
LOCUS       DAOONL010000196         4983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_20320_length_4983_cov_16.560065, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000196 DAOONL010000000
VERSION     DAOONL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20320_length_4983_cov_16.560065"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLP50_16485"
     CDS             complement(<1..850)
                     /locus_tag="PLP50_16485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA53190.1"
                     /translation="MDSRQRSVVVRTALLVGVLVLGVRAAAWAELESTALSEWYLWQE
                     TDEHVGLVAAARIASGNLLDQPPYRPWFSWQGVFGSPSDWEAVYPRNVYYQAPLYVYA
                     AAAAFRISDSPAVLLRLLQLLLAVAASTALAAAGTTVVLRTGRPSWCAVLAGSGAGLL
                     LGCYGPLVFLDGFLLRDGPLVSLSALLLALPLLGPSSPRTREILALGVLGGVSTLLKQ
                     TVLPLALAAGVVVVLREESRRRRLRSAVAFGAAFILPLVPLVARNLAVGAPAFAFDTR
                     PAVCFPW"
     gene            complement(855..1241)
                     /locus_tag="PLP50_16490"
     CDS             complement(855..1241)
                     /locus_tag="PLP50_16490"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPA53191.1"
                     /translation="MRRRPPRPPRLSIADFEKLVAEAVEGLPPEFREMLDNVVIAVEE
                     EPEEEDFVETDTPDDEELFGIYRGPMRTETAFDGLPGLPPQVVVFRGPILRCCASNRE
                     AVKEIQDTVRHELGHYFGLEDDEMPY"
     gene            complement(1238..2059)
                     /locus_tag="PLP50_16495"
     CDS             complement(1238..2059)
                     /locus_tag="PLP50_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPA53192.1"
                     /translation="MIELADSHGHVTMVFPGPEARWPTRAATDAEVAEQVARARDAGV
                     TRILVPATTLADAPRAVEVAERHDGVYAAVGVHPHETTGFDEDRVVSALERLASSSKV
                     VAIGEIGLDYFYDLAPKDDQKRALVAQLALAERLGKPVLLHNRESEEDMLPILEAHAK
                     GSPERPRGVFHSFCASTATGEKAIALGYFLSYSGMITFRMADNVRASAAVLPLESMLV
                     ETDAPYLAPAPFRGKPNEPAFVTRTAAKLAEVKGVTPEEVGRVTTAGFRRLFGTP"
     gene            2124..4013
                     /locus_tag="PLP50_16500"
     CDS             2124..4013
                     /locus_tag="PLP50_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53193.1"
                     /translation="MKNVVARLRSRPPLAAALLVLLVLLATTDERSFGTIPDGMQMLS
                     AGAAFSFSGEIGVSRAFATAAPRDGGDSSSRYGMGLSLLYAPFFWIARALHAVSPGLP
                     TTPVFALLPILSLSACAWGVARAAANLGAAPLAQWLAGSASILATPLWAYAGSDFSEP
                     LQVALVASSLAALTALRTGPSLRRALLAGALLGSLPLVKSSLWLVSAPLLVVAALSRP
                     ASAAPAPRRKAPGRPAAVMLVLRGVLLGGLPPVALWAALDLVRFRGVLGGYPGEGFSH
                     PLVDGLLKLAVYPNKGLLVYAPLTLFAIPGLIVLRKRDPALAIGLLISASALVLSGAT
                     WWAWEGQASWGPRLVLPAVPIAVLASVLFLGTARAGVALALALAGALVNLPGALQPFP
                     AVYALAATAHPGPHPGAPAGEERAEVYRGPDGRWLASAPYLLAVEPGWWPPLVHVRLL
                     RERLSGGDVGGRLESGALRLAPPFRPRRPAAPSAVYRQAVSSFSWPFWGRSFLAVEPG
                     LVDPLAFALRDQGIRDLESGNPRRADDRFRLLLEREGPDADPRSLALAADAAAGAGDP
                     ARARELLGRCPDPCHPWVLFVHEKLGDEMPGCPSSSRSAGNEASAGRRGPEIRPVSAW
                     ARETG"
     gene            complement(4076..4945)
                     /locus_tag="PLP50_16505"
     CDS             complement(4076..4945)
                     /locus_tag="PLP50_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPA53194.1"
                     /translation="MAGRPVPPVPEDPAVRENLERILASPSTLRAHEDLAWLARDELR
                     GSRLELELERAEAIISEAGVTGTVVVFGGTRVVERPDAERRLAEAEAAAAAAPGDAEA
                     LRAVERARRVLAKARYYDEAREFARIASSVCQRDGRCDFAIVTGGGPGVMEAANRGAF
                     DVGATSLGFNIVLPHEQFPNPYITPGLCIYFHYFATRKMHFLIRAKALVAFPGGYGTL
                     DELFETLTLIQTRKMPPVPVVLFGREFWERAIDLDFLVEEGTIAPEDARLVSYAETAA
                     EAWETISRFYGIG"
BASE COUNT          652 a         1863 c         1743 g          725 t
ORIGIN      
        1 cccatgggaa gcagacggcc ggccgcgtgt cgaaggcgaa cgcgggcgcc ccgactgcga
       61 ggttcctcgc gacgagcggg acgaggggca gaatgaaagc ggccccgaat gccacggcgc
      121 tccggaggcg acgccggcgg gactcctccc tcaggacgac cacgacgccg gccgcaagcg
      181 cgagagggag gaccgtctgc ttgagcagcg tcgatacgcc tccgaggaca ccgagggcca
      241 ggatttcccg cgtccgaggc gaggacggac ccaggagcgg aagggccagg agcagggccg
      301 aaaggctgac gagcggcccg tcgcgcaaga ggaagccgtc caggaagacg agaggtccat
      361 agcacccgag gaggaggccc gcaccggaac ccgcgaggac cgcgcaccac gagggacgcc
      421 ccgtgcggag gacgaccgtc gtgccggcag ccgcgagcgc tgtcgaggcg gcgacggcga
      481 gcaggagctg aaggagacgg agcaacaccg cgggcgagtc ggagatccgg aacgcggccg
      541 ccgcggcata gacgtaaagc ggcgcctggt agtagacgtt cctcgggtag acggcctccc
      601 agtccgacgg gctcccgaac accccctgcc aggagaacca gggacggtat ggaggctggt
      661 cgaggagatt cccggaggcg attctggcgg ccgcgacgag cccgacatgc tcgtccgtct
      721 cctgccagag ataccactcc gagagggccg tggactcgag ctccgcccac gcagccgcac
      781 gcactcccag tacgaggacc ccgacgagga gagccgtccg cacgacgacg gaacgctgcc
      841 gcgagtccat cgcctcagta tggcatctcg tcgtcctcga gcccgaagta gtgaccgagc
      901 tcgtggcgga cggtgtcctg gatctccttc accgcttccc ggttgctcgc gcaacagcgg
      961 aggatcggac cgcggaagac cacgacctgc ggcgggaggc ccgggaggcc gtcgaacgcg
     1021 gtctcggtcc gcatcggccc gcggtagatc ccgaagagct cctcgtcgtc cggcgtgtcc
     1081 gtctcgacga agtcttcttc ctcgggctcc tcctccacgg cgatgacgac gttgtcgagc
     1141 atctcccgga actcgggcgg gagcccctcg acggcctcgg cgacgagctt ctcgaaatcc
     1201 gcgatcgaga ggcggggcgg acgtggcggg cggcgtctca cggcgtcccg aagagcctcc
     1261 ggaagccggc ggtcgtcacg cgcccgacct cctccggcgt gacgcccttc acctcggcga
     1321 gcttcgcggc ggtccgcgtc acgaaggccg gctcgttcgg cttcccccgg aacggcgcgg
     1381 gcgcgaggta gggcgcgtcc gtctcgacga gcatcgactc cagcggaagg accgcggcgg
     1441 acgccctcac gttgtcggcc atccggaacg tgatcatccc cgagtagctg aggaagtagc
     1501 cgagcgcgat cgccttctcc cctgtcgccg tcgaagcgca gaacgagtgg aagacgccgc
     1561 gcggcctctc cgggctcccc ttcgcgtgcg cctcgaggat cgggagcatg tcctcctccg
     1621 actcgcggtt gtggaggagg accggcttgc cgagccgctc ggcgagcgcg agctgcgcga
     1681 cgagcgcccg cttctggtcg tccttcgggg cgaggtcgta gaagtagtcg aggccgatct
     1741 ccccgatggc gacgaccttc gacgaagacg cgagccgctc gagcgcggag acgacccggt
     1801 cctcgtcgaa ccccgtcgtc tcgtgcgggt ggacgccgac ggccgcgtac accccgtcgt
     1861 gccgctcggc gacctccacc gccctcggcg cgtccgcgag ggtcgtcgcc gggacgagga
     1921 tccgcgtcac cccggcgtcc cgcgcccgcg cgacctgctc cgccacctca gcgtcggtcg
     1981 cggcgcgtgt cggccaccgg gcctccggac ccgggaagac catcgtcacg tgcccgtggg
     2041 agtctgcgag ctcgatcacg ggccggagga tagcggacgg agccttccgc gtcggtccgc
     2101 ctccggctaa tctccgcgca cccatgaaga acgtggtcgc ccggcttcga agccggccac
     2161 ctctcgcggc ggccctgctg gtgctcctcg tcctcctggc gacgacggac gagcgctcgt
     2221 tcggaacgat ccccgacggt atgcagatgc tgtctgccgg cgcggccttc tcgttctccg
     2281 gcgagatcgg cgtctcccgg gctttcgcca cggcggctcc tcgcgacggg ggagactcct
     2341 cgtcccggta cgggatgggt ctcagcctcc tctatgcgcc gttcttctgg atcgcccggg
     2401 ccctccacgc cgtctccccg ggcctcccga ccacgcccgt cttcgccctc ctgccgatcc
     2461 tctcgctctc cgcctgtgcc tggggtgtgg cgcgggcggc ggcgaacctc ggagccgctc
     2521 cccttgcgca gtggctcgcc ggctcggcga gcatcctcgc gacgcctctc tgggcctacg
     2581 ccggaagcga cttctcggag ccgctgcagg tcgccctcgt cgcgtcctcc cttgcggcgc
     2641 tcacggcgct ccgcaccggg ccttctctgc gccgcgcgct cctggccggc gccctgctcg
     2701 ggtcgctgcc gctcgtgaag tcgtcgctgt ggctcgtttc cgcgcctctc ctcgtggtcg
     2761 cggcgctgtc gagaccggcc tccgccgctc cggctccccg ccgcaaagca cccggccggc
     2821 ccgcggccgt catgctggtc ctgcgcggcg tccttctcgg cggtctcccg cccgtcgcgc
     2881 tgtgggccgc gctcgacctc gtgcgcttcc gaggcgtcct cgggggctac ccgggcgagg
     2941 ggttctccca tccgctggtc gacggcctcc tgaaactcgc cgtctacccg aacaagggcc
     3001 ttctcgttta cgcgccgctc acgctcttcg cgatccctgg cctgatcgtc ctccggaagc
     3061 gggacccggc gctcgcgatc ggcctcctca tctcggcctc agccctcgtc ctgtccggcg
     3121 cgacatggtg ggcctgggaa ggacaagcgt cctggggtcc acgtctcgtg ctgcccgccg
     3181 tgccgatcgc cgtgctcgcg agcgtcctgt tcctcgggac cgcgcgagcc ggggtggcgc
     3241 ttgcgctcgc cctcgcgggc gcgctcgtga acctgccggg cgctcttcag cccttccctg
     3301 cggtctacgc gctcgccgcg accgcccacc cggggcccca cccgggggca ccggcaggag
     3361 aagagcgggc cgaggtgtac cgaggtccgg acgggcgctg gctcgcctcg gcgccctacc
     3421 tcctcgcggt cgagcccgga tggtggcctc ctctcgttca cgtccggctc cttcgagaac
     3481 ggctgtcggg aggagacgtc gggggtcggc tcgagagcgg tgccctccgg ctcgcgcctc
     3541 ccttccggcc caggcggccg gcggccccct ccgcggtcta ccgccaggcg gtctcctcct
     3601 tctcgtggcc gttctggggc cggtcgttcc tcgcggtcga acccgggctc gtggatccgc
     3661 tggcattcgc tctccgggac cagggaatcc gcgatctcga gtccggcaac ccccgccgcg
     3721 ccgacgaccg cttccggctg ctcctcgagc gtgagggtcc ggatgcggat ccgcgcagcc
     3781 tggccctcgc ggccgacgcc gccgcggggg cgggggatcc cgcccgtgcc cgcgagctcc
     3841 tcggccgctg cccggacccg tgtcacccgt gggtcctctt cgtgcacgag aagctgggcg
     3901 acgagatgcc ggggtgtcct tcctcgagcc gttcggccgg gaacgaggct tccgcaggac
     3961 ggcgcggccc ggagatccga cccgtcagcg cctgggcccg ggaaaccggc tgactcgcga
     4021 tccctcggac acgagccggg gccgcgcccg gcccggtgcg gcgacgctcg gcctctcagc
     4081 cgatcccgta gaaccgggag atcgtctccc acgcctcggc cgcggtctcg gcgtacgaga
     4141 ccaggcgcgc gtcctcgggc gcgatcgtcc cctcctcgac gaggaagtcg aggtcgatcg
     4201 ccctctccca gaactcgcgc ccgaagagaa cgaccgggac gggcggcatc ttccgcgtct
     4261 ggatgagcgt cagcgtctcg aagagctcgt cgagcgtgcc gtacccgccc gggaaggcga
     4321 cgagcgcctt cgcccgaatg aggaagtgca tcttccgggt cgcgaagtag tggaagtaga
     4381 tgcagaggcc gggcgtgatg tacgggttcg ggaactgctc gtgcgggagg acgatgttga
     4441 agccgaggct cgtcgccccg acgtcgaagg cgccccggtt cgcggcctcc atgacgcccg
     4501 ggccgccgcc ggtgacgatg gcgaagtcgc agcgtccgtc ccgctgacag acggaggagg
     4561 cgatcctcgc gaactcgcgc gcctcgtcgt agtagcgcgc cttcgcgagg acgcgccgcg
     4621 cccgctcgac ggcgcggagg gcctccgcgt cgcccggcgc ggccgcagcc gccgcctcgg
     4681 cctccgcgag gcggcgctcg gcatccggcc tctcgacgac gcgcgtgcct ccgaagacga
     4741 cgaccgtacc ggtgacgccc gcctccgaga tgatcgcctc ggcccgctcg agctcgagct
     4801 cgaggcgcga gccgcgcagc tcgtcccgcg cgagccaggc caggtcctcg tgcgcccgga
     4861 gcgtgctcgg cgaggcgagg atcctctcga ggttctcgcg gacggccggg tcctccggaa
     4921 cgggcgggac gggccggccg gccactactt caccttcgtc ccggagggga ccgacgggtc
     4981 gac
//
LOCUS       DAOONL010000197         4969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_20400_length_4969_cov_24.615792, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000197 DAOONL010000000
VERSION     DAOONL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20400_length_4969_cov_24.615792"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2532
                     /locus_tag="PLP50_16510"
     CDS             <1..2532
                     /locus_tag="PLP50_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPA53195.1"
                     /translation="AVDEQVIKALPVGQEYRDLVKLVPGVQYSEDSVRGPSAGGSGQD
                     NVYSFDGVNVNLPLFGTLSADPSSHDIAQVAVVKGGADATEFNRAAGITVNTVSRSGT
                     NAFRGSLSYQMQPESLIADRDTTSSAVFEEDKDWAVANIGGPVFKDRLFFYASYYSPT
                     STRDNRANLYGEVPNYKSTRDEIFGKLTYSPTDSILLNGSYRHSDREESHYGVGGEAY
                     AASTSNGAKAKQKIAIFEGSWIINDRSYATAKFTDFRNPNTGRPDTLLSVVPSMGAGT
                     TLNVGALDTMGFVAIPVPITGQTAFNDFIAPIIDRYGYLQDGVRKGGGRIGAGSQFDN
                     NDFYRQSYQLGYDVSFGQKVTHNLHVGYQWSKDQEDLYRTSNGWGSITIPGGRLNCPA
                     KSTCAGQPIYYQAQVQQQGILNVPTINSQYHQQNIELNDSIRWANVTVNVGLLASNDK
                     LYGMGLREKEGTVSGFEVAQGSRYLMHEIKFGDALQPRLGIVWAYQGANTVYANYARY
                     IPTAGSLPRAASWARSSAAIINVYFDENGNFIGSTPEASSRGKIFQDGIKPRKTDEYM
                     IGTTKDLGMGWTGRLHGRYRKSDNFWEDTNNNARLVFNPPPGIPQELYVPNLPEINKE
                     LGATSYVIAALDGAFTKYYEAGLEAEWRGRNAYVRGSYVWSHYYGNFDQDNSAGSGTG
                     NDSNIFIGSSNIGDGAGRQLWNFKYGNLRGDRRHQLKLYGSYQLPWRSSVGAYAVYQS
                     GQPWEYHSYEPYISQTTSTSDSNRYAEPAGTRRTDDHYQMDLNYTQTIPIGDLFRLEA
                     RIDVFNLFDRQTGYNIQDNVHSANPGTPQTFYDPRRVQFALKLEF"
     gene            2633..3568
                     /locus_tag="PLP50_16515"
     CDS             2633..3568
                     /locus_tag="PLP50_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPA53196.1"
                     /translation="MPSLYAGVDGGGTRTRAVVTAPDLLPLGRGASGPTNASVVPVPH
                     IVQSVAESVDDALEAAGASRADLALVSCGLAGVDASATAGRLVAALEAVFGPGRVRVT
                     TDARIALAGALPDPVGSAGAVLIAGTGAICFGRNAHGLEERAGGWGALIGDEGSASEI
                     ARRGLAAVARDVDGRGPRTRMREALDATEGTRSAVETIRRLFRPGAGPTDTAAYFPVV
                     LGAARAGDAIALGILEWAGRELAGTTLAVLRKLGIAGEEPTVATVGGVFSAGELVLAP
                     LRERLLEGSPRTRLGPPAHIPEIGAIRLALAEGAR"
     gene            3565..4410
                     /locus_tag="PLP50_16520"
     CDS             3565..4410
                     /locus_tag="PLP50_16520"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPA53197.1"
                     /translation="MTSTLARLETIHAADLEAVRAVGKVLPDVARAVEEIARRLEAGG
                     RWLNVGAGTSGRLGVLDASELPPTFGVSPSLVTGRIAGGRAALVDAVEGAEDDEAAGA
                     AEIRAEGLGGNDVVLGIAASGATPYVAGALVAAKAAGALAVALVCAPGSRLAALADLA
                     LVVETGPEVLRGSTRMKAGTAQKLVLNMLSTAVFARLGLVWRGEMVAMRPTNAKLRQR
                     AIDILGRTLGVADEEARALLSETGWELPPALVAARWRVSPSRASEWIAACRGNVARAL
                     EEEPS"
     gene            4407..>4969
                     /locus_tag="PLP50_16525"
     CDS             4407..>4969
                     /locus_tag="PLP50_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPA53198.1"
                     /translation="MTGAGLVDLHVHGAFGVDLLTADEAELDRLALGLSARGVEGFVP
                     TLVPVPLGELAPLVARLSAWVRSRTSGDGRGAMPLGIHLEGPFVSPNRSGALHRGCLL
                     DGSDPRTVDAFFDAVGEVPGRPVVTMAPEVPGGLDLVSRFVARGFLVSLGHTEADVPT
                     LDEALRRGARHMTHFGNAMKALHHRDAG"
BASE COUNT          753 a         1910 c         1644 g          662 t
ORIGIN      
        1 gccgtcgacg agcaggtcat caaggccctg ccggtcggcc aggagtaccg cgacctcgtg
       61 aagctcgtcc ccggcgtcca gtactccgag gactccgtcc gcggcccgag cgcgggcggc
      121 agcggccagg acaacgtcta cagcttcgac ggcgtgaacg tgaacctccc gctcttcggc
      181 acgctctcgg ccgacccctc ctcgcacgac atcgcgcagg tcgcggtcgt caagggaggc
      241 gccgacgcca ccgagttcaa ccgcgcggcc ggcatcacgg tgaacaccgt cagccgctcg
      301 ggaacgaacg ccttccgcgg cagcctctcg taccagatgc agccggagag cctgatcgcc
      361 gaccgcgaca cgaccagctc cgccgtcttc gaggaggaca aggactgggc ggtcgccaac
      421 atcggcggcc cggtcttcaa ggaccgcctc ttcttctacg cttcctacta cagcccgacg
      481 agcacgcgtg acaaccgcgc gaacctctac ggcgaggtcc cgaactacaa gagcacgcgc
      541 gacgagatct tcggaaagct cacctactcg ccgaccgact cgatcctcct caatggctcc
      601 taccggcact cggaccgcga ggagagccat tacggcgtcg ggggcgaggc ctacgccgcc
      661 tcgacgagca acggcgcgaa ggcgaagcag aagatcgcga tcttcgaggg ctcctggatc
      721 atcaacgacc ggagctacgc gacggcgaag ttcaccgact tccgcaaccc gaacaccggg
      781 cggcccgaca cgctcctcag cgtcgtcccg tcgatgggag cgggcacgac cctcaacgtc
      841 ggcgccctcg acacgatggg cttcgtggcc atccccgtcc ccatcacggg gcagaccgcc
      901 ttcaacgatt tcatcgcccc gatcatcgac cggtacggct acctgcagga cggcgtccgc
      961 aaggggggcg gccggatcgg cgccgggtcg cagttcgaca acaacgactt ctaccggcag
     1021 agctaccagc tcggctacga cgtctcgttc ggtcagaagg tgacccacaa cctgcacgtc
     1081 ggctaccagt ggtcgaagga ccaggaggac ctctaccgga cctccaacgg ctggggctcg
     1141 atcacgatcc ccggcggccg gctcaactgc ccggcgaagt ccacctgcgc gggtcagccc
     1201 atctactacc aggcccaggt gcagcagcag gggatcctga acgtcccgac gatcaactcg
     1261 cagtaccacc agcagaacat cgagctgaac gactcgatcc gctgggcgaa cgtcaccgtc
     1321 aacgtcggcc tcctggcgag caacgacaag ctctacggca tgggcctccg cgagaaggag
     1381 gggaccgtct cgggattcga ggtcgcgcag ggcagccgct acctgatgca cgagatcaag
     1441 ttcggagacg cgctccagcc gcggctcggc atcgtctggg cctaccaggg ggcgaacacc
     1501 gtctacgcga actacgcccg ctacatcccg acggccggct cgctcccgcg agcggcgtcc
     1561 tgggcccgga gctcggcggc catcatcaac gtctatttcg acgagaacgg caacttcatc
     1621 ggctcgacgc cggaggcctc gtcgaggggc aagattttcc aggacggcat caagccccgg
     1681 aagaccgacg aatacatgat cgggacgacg aaggacctcg ggatggggtg gaccggccgc
     1741 ctgcacggcc gctaccggaa gtccgacaac ttctgggagg acacgaacaa caacgcgcgc
     1801 ctcgtcttca acccgccccc cgggatcccg caggagctct acgtcccgaa cctccccgaa
     1861 atcaacaagg agctcggcgc cacgtcctac gtcatcgccg cgctcgacgg cgccttcacg
     1921 aagtactacg aggcgggcct cgaggccgag tggcgggggc ggaacgccta cgtccgcggc
     1981 tcctacgtct ggagccacta ctacggcaac ttcgaccagg acaactccgc cggctccgga
     2041 acgggcaacg acagcaacat ctttatcggc tcgtcgaaca tcggcgacgg cgccggccgc
     2101 cagctctgga acttcaagta cggcaacctc cgcggcgacc gccggcacca gctcaagctc
     2161 tacggctcct atcagctccc ctggcggagc tccgtcggcg cctacgcggt ctaccagtcg
     2221 ggccagccgt gggagtacca cagctacgag ccgtacatct cgcagacgac cagcacgagc
     2281 gactcgaacc ggtacgccga gccggccggc acccggcgga ccgacgacca ctaccagatg
     2341 gacctgaact acacgcagac gatcccgatc ggcgacctct tccggctcga ggcccgcatc
     2401 gacgtcttca acctcttcga ccggcagacc gggtacaaca tccaggacaa cgtccactcg
     2461 gccaaccccg gcacgccgca gacgttctac gacccgcggc gcgtgcagtt cgccctcaag
     2521 ctcgagttct agccgacgaa ccgagaccga cgaagggccc gcggagcgat ccgcgggccc
     2581 tttccttttc tcctccccgg accgggacgc tcccggcggt acgattcgct ccatgcccag
     2641 cctctacgcc ggcgtcgacg gcggcgggac acggacccgc gccgtcgtca cggcccccga
     2701 cctcctgccg ctcggtcgcg gcgcctccgg ccccacgaac gcctcggtcg tccctgtccc
     2761 ccacatcgtc cagtcggtgg cggagtcggt cgacgacgcc ctcgaggccg ccggcgcttc
     2821 ccgcgccgac ctcgcgctcg tttcctgcgg cctcgccgga gtcgacgcct cggcgacggc
     2881 cgggcggctc gtggccgcgc tggaggcggt cttcggtccc ggccgcgtcc gcgtgacgac
     2941 cgacgcgcgc atcgcccttg ccggggccct tcccgatccg gtcggaagcg ctggagcggt
     3001 cctgatcgcc ggcaccggcg cgatctgctt cggccgtaac gcccacggcc tcgaggagcg
     3061 cgccggtggc tggggcgccc tgatcggcga cgagggctcc gcctcggaga tcgcccggcg
     3121 cggcctcgcc gccgtggctc gcgacgtcga cggccgcggc ccccggacga ggatgcggga
     3181 agcgctcgac gccacggagg ggacccgctc ggccgtcgag acgatccggc gcctcttccg
     3241 tcccggcgcc gggcccaccg acaccgccgc ctacttcccg gtcgtcctcg gcgccgcgcg
     3301 cgcgggcgac gccatcgccc tcgggatcct cgagtgggcc ggccgcgagc tcgccgggac
     3361 gacgctcgcc gtcctccgca agctcgggat cgccggcgag gagccgaccg tcgccacggt
     3421 cggcggcgtc ttctcggcgg gcgagctcgt cctcgccccc ctccgcgagc gtctcctcga
     3481 gggctccccg aggacccggc tcggcccgcc ggcgcacatc ccggagatcg gcgccatccg
     3541 cctcgccctc gccgaggggg cgcggtgacg tcgacgctcg cgcggctcga gacgattcac
     3601 gcggccgacc tcgaggccgt ccgggccgtc gggaaggtcc tccccgacgt ggcgcgcgcg
     3661 gtcgaggaga tcgcccgccg gctcgaggcg ggaggacgat ggctgaacgt cggggccggg
     3721 acgagcggcc ggctcggggt cctcgacgcc tcggagctcc ccccgacgtt cggggtctcc
     3781 ccctcgctcg tcaccggccg gatcgcgggc gggagggccg ccctcgtcga cgccgtcgag
     3841 ggggcggaag acgacgaggc ggccggggcc gccgagatcc gcgcggaggg gctcggaggg
     3901 aacgacgtcg tcctcggaat cgcggccagc ggcgcgacgc cgtacgtcgc gggcgcgctc
     3961 gtcgccgcga aggcggccgg cgccctcgcg gtcgccctcg tctgcgcgcc cggctcgcgc
     4021 ctcgccgcgc tcgccgacct cgcgctcgtc gtcgagaccg gtccggaggt cctccgcggc
     4081 tcgacgcgga tgaaggcggg gacggcgcag aagctcgtcc tgaacatgct ctcgaccgcc
     4141 gtcttcgccc ggctcggcct cgtctggcgc ggggagatgg tggcgatgcg gccgacgaac
     4201 gcgaagctcc ggcagcgggc gatcgacatc ctcggccgga cgctcggcgt cgcggacgag
     4261 gaggcgcgcg ctctcctgtc ggagacgggc tgggagctcc cgccggcgct cgtcgccgcg
     4321 cgctggcgcg tctccccgtc ccgcgcctcc gagtggattg ccgcgtgccg agggaacgtc
     4381 gcccgcgccc tcgaggagga gccgtcgtga cgggcgccgg cctcgtcgac ctccacgtcc
     4441 acggcgcatt cggcgtcgac ctcctgaccg cggacgaggc ggagctcgac cggctcgcgc
     4501 tcggcctttc ggcccgcggc gtggagggct tcgtcccgac gctcgtcccc gtcccgctcg
     4561 gggagctcgc cccgctcgtg gcccgcctct cagcgtgggt ccgctcccgg acgtccggcg
     4621 acgggcgcgg ggcgatgccg ctcggaatcc acctcgaggg gccgttcgtc tcgccgaacc
     4681 gctccggcgc cctccaccga ggctgcctcc tcgacgggag cgatccgcgc acggtcgacg
     4741 ccttcttcga cgccgtcggc gaagtccccg gccggcccgt cgtcacgatg gccccggagg
     4801 tccccggagg cctcgacctc gtctcgcgct tcgtcgcgcg cggcttcctc gtctccctcg
     4861 gccacaccga ggccgacgtc ccgacgctcg acgaggcgct ccggcgcggc gcgcggcaca
     4921 tgacccactt cgggaacgcg atgaaggcgc tccaccaccg cgacgcggg
//
LOCUS       DAOONL010000198         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_20669_length_4915_cov_11.138477, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000198 DAOONL010000000
VERSION     DAOONL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20669_length_4915_cov_11.138477"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLP50_16530"
     CDS             complement(<1..234)
                     /locus_tag="PLP50_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53199.1"
                     /translation="MLPYLASKDGRFGAVVQPLSGLTLRALAVTVKRGRSPEAPVRAD
                     VPVREDEPVPVAKVQHNHVMALLTTADAHMFEPR"
     gene            complement(749..985)
                     /locus_tag="PLP50_16535"
     CDS             complement(749..985)
                     /locus_tag="PLP50_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53200.1"
                     /translation="MPERRRKPAVGLRIEAKLPNTNQKRGIGTNTTGVLLSVTFQKAP
                     GRLLVITDLGLGTLIEPTDEQAQNDVLLCGLAAA"
     gene            complement(1077..2396)
                     /locus_tag="PLP50_16540"
     CDS             complement(1077..2396)
                     /locus_tag="PLP50_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA53201.1"
                     /translation="MTEAINVSPNDLESADGRRRVGRSSLERHPAFTLAIVEFDDQGY
                     FWDRRQVDTLEEEIRREANRPGVHGASIAVFVHGWQHTADVCDSNLCCFRESLRLAAE
                     DQKTALAFSGTGREPVPLFGVYIGWRGRSKRGWPLNHLSFFSRKNAALKVGEGDMTEF
                     LTRLDCLRNELIASGKGRSRLVIAGHSLGATVVYSAISGVLKSRFAEAQRTPGPSLGR
                     ANVIRGFGDLVVLLNPALDAVSFHPLWEMASRFTALSRTQAPVLVVVGSETDSATGSF
                     FPIGQTIGTLFERTRDTEQRRALRTAVANYEPYVSHRLFAADPELGGLVPEPRVEACY
                     CSLPYRRISRPEAIELMSMAEALERQDGAETGARTGPSWGEATCEPERVLGRTRLSCV
                     KPVRRGFPIWVVRAAPEVLHEHNGFSILLGDFIRRLLLGGAIEPAIP"
     gene            complement(2495..3418)
                     /locus_tag="PLP50_16545"
     CDS             complement(2495..3418)
                     /locus_tag="PLP50_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53202.1"
                     /translation="MAACRHRSLALTLLPLRQAAGRPEESLAGKPEGAGEFVPVPPVV
                     GIPGPLFFETPTLTFVPKGTIRVTAGVRWLNEVEYPYSGLSGDLAQLPVVRVDVGFGS
                     RIEVRVQGAARQRLRIDWDASDVHPPAEVSGDATADAGDFSVVTIAQVMPERGWKAAF
                     GLRVEVKLPNTSEKAGIGTNTTDVLLSVPAQKRLGRLVFNSDVGVGILTQPTSAQRQT
                     DVLLYGLAAAYEASPAWMLSAEVNGRWTSSGGKPGTGNHSTVRMGASWSTGPVAVGLV
                     LSRGFGEGGERFGGLLAVSYGFRIYDRVRAE"
     gene            complement(3423..4862)
                     /locus_tag="PLP50_16550"
     CDS             complement(3423..4862)
                     /locus_tag="PLP50_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPA53203.1"
                     /translation="MYHKLTPHAAVLVSATLLLGGCTMMPRYEQPPLPVADSFKAEAN
                     ASGAAVTAPTAASPATAPAAPDVPWREYFTDARLRSVIDLALANNRDLRVSTLNVERV
                     AALYRIQRSELFPTIGVQATGERYRVPESLGENGKASTNSSYTVGLGMASWELDLFGR
                     IRSLNARSLEQYLATEEARRGAQTSLVAAVASTWLSLAASEENLRISQATLEAQRASH
                     DLIRQSRDAGIGSDLELSQAQSQVEAARAAVASFTGAVGVARNALDLLVGAPVPADLL
                     PERLAAVTDPKGLEAGLPSDVLLRRPDILAAEHLLRGANANIGAARAAFFPRISLTAG
                     FGSSSPDLDGLFKSGTNAWSFAGQIVSPLFASGSLIANLKVSKLDRQIAVAQYEKAIQ
                     AAFAEVNDGLTLRTTLVEQRDAQDALVKSLEDFSRLSDARYKAGLDGYLGVLVAQQAL
                     LNAQRAAVGARLAEQANLVTLYKVLGGGV"
BASE COUNT          817 a         1612 c         1715 g          771 t
ORIGIN      
        1 ccgaggctca aacatgtggg cgtccgcggt ggtcagcagc gccatgacgt ggttatgctg
       61 gaccttggcc accggaaccg gctcgtcctc ccgcaccggc acgtcggcgc ggaccggcgc
      121 ctcgggcgac cggccccgct tgaccgtgac cgcgagggcc cggagggtca gacctgagag
      181 cggctgaacg acagcgccga accgcccgtc cttgctggcg aggtaaggca acacccctcc
      241 cttaccgatt ctcggtcgcc tggcgatagc aggggtcagg gtctccccgg agtggaacgg
      301 cacgagcggt ttcacggcgg gacgtagtag ggcttcgggg cgtgcgacgt gacccattga
      361 ccagaatggt atctgagtca cagcgaaacc tcaagcgacg tgccgcaacg tttcacgccg
      421 gtcttctctt cacaagcgca ccgggccact tcccttgaga gcgtcccccc gcgctgtctc
      481 tgagctctcg ggaccccgaa tgcggcgccc atcctccgcc gggatgactc tctcgaagac
      541 ggagaaaccg aatgagacgg caggcgacac gcagaagcgc gcggcgtcgg ccgacaggcc
      601 ccgggagagg atggccgcta cggtcggcgc gtcgaacgag taggactcgc cggcgcgaag
      661 gacggaatgg tcgcccgtgc cgggatgctc ggctgacggc gaaccgcggt cagcgaggtc
      721 gtcggaagcc tgatcttcgg ctcgccgtct atgcggctgc caagccgcag aggaggacgt
      781 cgttctgcgc ctgctcgtcg gtcggctcga tcagggtgcc gaggcccaga tcggtgatga
      841 cgaggaggcg gccgggagcc ttctggaacg tgactgagag gaggacgccc gtcgtgttcg
      901 tcccgatgcc gcgcttctga ttcgtgttcg gaagctttgc ctcgatcctc agtccgacgg
      961 cgggcttccg cctcctttcc ggcattaccc ggcgatggtg atgacggaga agtcgccggc
     1021 gtcgtgagtt gaggaaccgt tggcgtttct taccgtacga cacgctagag accggctcac
     1081 ggaatcgccg gctcaatcgc gcctccgaga agcagccgac gtatgaagtc gcccaggaga
     1141 atagagaagc cattgtgctc gtgaagaacc tccggtgccg cgcggacgac ccagatcggg
     1201 aagccgcgac ggacgggttt cacgcaggaa aggcgagttc gaccgagcac gcgttcgggt
     1261 tcgcacgtcg cctcgcccca agagggaccg gttctcgccc cggtctccgc gccgtcctga
     1321 cgctcgagtg cttcagccat actcatgagt tcgatcgcct ccggcctcga gattcgccga
     1381 tatgggagag agcagtagca agcctcgacc ctcggctcag gcacgagccc gcccaactca
     1441 ggatccgcgg cgaagagccg gtgggagacg taaggttcgt agttagcgac cgccgtacgt
     1501 agggcccgcc gttgctcggt gtcgcgcgtc ctctcgaaga gggttccgat agtctgacct
     1561 atcgggaaga agctccccgt cgcgctgtcc gtctcggaac ccaccaccac gaggacaggg
     1621 gcctgcgtcc gagaaagcgc cgtgaacctg gaggccatct cccagagcgg gtgaaaggac
     1681 accgcatcaa gggccggatt gaggaggacg acaaggtccc cgaagccgcg gatgacgttc
     1741 gcccgcccga gcgatggacc gggcgtccgc tgcgcctcgg cgaaccggct cttcaggact
     1801 cccgagatcg ccgagtagac gacggtcgcg cccaggctgt gccctgctat gacgagacga
     1861 gaacgtccct tcccagaggc aatcagctcg ttgcgaaggc aatcgaggcg ggtgaggaat
     1921 tccgtcatgt cgccctcgcc gactttgagc gcagcgttct tcctcgagaa gaaggagaga
     1981 tggttcagcg gccagcccct cttcgagcgc cccctccagc caatgtagac gccgaagagt
     2041 ggcaccggct cccggcccgt cccgctgaac gcgagggccg tcttctggtc ctccgcggcg
     2101 agccggagcg attcgcggaa gcaacagaga ttcgaatcgc agacgtcggc ggtgtgctgc
     2161 caaccgtgaa cgaagacggc gattgaggcc ccgtgcacgc ctggacgatt cgcctcgcgc
     2221 cggatctcct cttccagtgt gtcgacctgc cgtcgatccc agaagtagcc ctgatcgtcg
     2281 aactcgacga tcgccaaggt gaaggccggg tggcgctcga gtgacgagcg cccgactcga
     2341 cgcctgccat cggcgctctc caggtcgttc ggtgagacgt tgatggcctc cgtcacagac
     2401 cggaaaggtc ggttcggcgt ggcgcatccg gcggggagcg cgaggagaag cgcgaggagg
     2461 agaaaccgaa cgggcgcctc gcgacaggac atagtcattc ggctcggacg cgatcgtaga
     2521 tgcggaagcc gtatgaaacg gcgagcaagc cgccgaacct ttcgcccccc tccccgaacc
     2581 cgcgggacaa cacgaggccg acggcgacgg ggcctgtgga ccacgaggcg cccatccgta
     2641 cggtcgagtg gttccccgtc ccgggcttcc ccccggacga cgtccagcgc ccattgacct
     2701 cggccgagag catccatgct gggctcgcct cgtacgccgc cgcgaggccg tagaggagca
     2761 cgtccgtttg tcgctgggca ctcgtcggct gcgtcagaat gccgacgccg acatcagagt
     2821 tgaagacgag cctcccgagg cgcttctgtg ctgggacgga gagaaggacg tccgtcgtgt
     2881 tcgttccgat ccctgccttt tcgctcgtgt tcgggagctt cacctctacc cgcaacccga
     2941 aggccgcctt ccagccccgc tccggcatga cctgcgcgat cgtgacgacg gagaaatcgc
     3001 cggcgtcggc tgtcgcgtcg cccgagacct ccgccggagg gtggacgtcc gaggcgtccc
     3061 aatcgatccg gagacgttgc cgggccgccc cttggacgcg gacctcgatg cggctcccga
     3121 agccgacgtc gacacgaacg acggggagct gcgcgagatc gccggagagg cccgagtagg
     3181 ggtactcgac ttcgttaagc cagcggacgc ccgccgtcac gcgaatcgtc cccttcggaa
     3241 cgaacgtgag tgtcggtgtc tcgaagaaca gcggacccgg gatcccgacg acaggcggaa
     3301 ccgggacgaa ctctcccgcc ccctcgggct tgccggccag agactcctcc ggcctgccgg
     3361 ccgcctggcg gagcgggagc agcgtcagag cgagagagcg atggcggcaa gccgccaccg
     3421 cttcacacgc cgcctccgag gaccttgtag agtgtgacga ggttcgcctg ctcggcgagg
     3481 cgggcgccca ctgcagctcg ctgggcgttg aggagcgcct gctgggctac gaggacgccg
     3541 agatatccgt cgaggcccgc tttgtaacgg gcgtcggaga gcctcgagaa gtcctcgagc
     3601 gacttcacga gggcatcttg ggcgtcgcgc tgctccacga gcgtcgtgcg gagcgtgagt
     3661 ccgtcgttca cctcggcgaa ggcggcctgg atcgccttct cgtactgcgc gacggcgatc
     3721 tggcggtcca gcttcgacac cttgaggttc gcgatgaggg agccgctcgc gaagaggggg
     3781 gagacgatct gcccagcgaa gctccaggcg ttcgtccccg acttgaagag gccgtcgagg
     3841 tcggggctcg agctgccgaa gccggccgtg agggagatgc gcggaaagaa cgccgcgcgg
     3901 gccgcgccga tgttcgcgtt cgcaccgcgg aggaggtgct cggcggcgag gatgtcggga
     3961 cgccggagaa ggacgtccga tggaaggccg gcctcgagcc ccttcgggtc cgtgacggcg
     4021 gcgagccgct ctgggaggag gtcggccggg acgggcgcgc cgacgaggag gtcgagcgcg
     4081 ttccgcgcga ctccgaccgc gccggtgaag gaggcgacgg ccgcccgggc cgcctccacc
     4141 tggctctgcg cctggctgag ctcgaggtcg gagccgattc ccgcgtcgcg gctctggcgg
     4201 atcaggtcgt gcgacgcacg ttgcgcctcg agagtcgctt gcgagatccg aaggttctcc
     4261 tcgctcgccg cgaggcttag ccacgtgctg gccaccgcgg cgaccaggga cgtctgcgcg
     4321 ccccgtcggg cctcctcggt ggcgaggtac tgctcgaggg accgggcgtt caggctccgg
     4381 atgcgcccga agaggtcgag ttcccacgag gccatgccga ggccgacggt gtaggacgag
     4441 ttcgtgctcg ccttcccgtt ctccccgagg ctctccggga cccggtacct ttcgcccgtc
     4501 gcctggacgc cgatcgtcgg gaagagctcg gagcgctgga tccggtagag ggccgcgacc
     4561 cgctcgacgt tgagcgtcga gaccctgagg tcgcggttgt tggcgagcgc caggtcgatg
     4621 acggatctga ggcgcgcgtc ggtgaagtac tctcgccagg ggacatccgg ggctgcaggc
     4681 gccgtcgcag gagacgccgc agtcggggcg gtgaccgctg ccccggaggc gttggcttcg
     4741 gccttgaagg aatctgccac cggcagcggc ggctgttcgt acctcggcat catcgtgcat
     4801 ccgcccagga gtaacgtcgc gctgacgagc acggccgcgt gaggggtcag cttgtggtac
     4861 atgtcacttt ccctccgccg gaagcggggc ctcgggcgcg ggtgtcatct cgtgc
//
LOCUS       DAOONL010000199         4889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_20820_length_4889_cov_14.656806, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000199 DAOONL010000000
VERSION     DAOONL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20820_length_4889_cov_14.656806"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLP50_16555"
     CDS             complement(<1..1288)
                     /locus_tag="PLP50_16555"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HPA53204.1"
                     /translation="MSKPFSFGAIDVRLESGSEPRPARAEDGAPFRILLAGDFSGRRA
                     RGLVETGTTLGERKAFRVDLDSLDATIARLEPQVRLRDGDVLRFRELDDFLPDTLFSR
                     IARFGALKDAAAAESRRRETARPAAGLLDRILDGTPQEETPVVEAGPDGPTELMRAIL
                     GSAAFRALEASWRGVDFLVRRLDVDGPLTLHLVDLSPEELRADLLVGDDLGRTGLHRL
                     LVEKTVGTPGGEPWAAFVLLDSFGPSREDAEALGRLARIAALAGTAVLAGTDPGLVGC
                     ASLAATPDPDDWTEPAGEGAEAWTALRRLPEARFVGLALPRLLLRLPYGKETNAVDAF
                     DFEELSSPPEHEQYLWGSPALATALLLGEAFLEDGWSLRPGSAQEISGLPFALVEEDG
                     EKRAVPCAEALFTVRAMQAVAEKGLMPLLTMKGTDTV"
     gene            1373..2353
                     /locus_tag="PLP50_16560"
     CDS             1373..2353
                     /locus_tag="PLP50_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53205.1"
                     /translation="MIRFLKKVGRQLPVLKEIHRYILALEAEREELRGTVATQREELV
                     RARETTRKLWVPPGDPRSPIPDLDDVRRRESELFGRPPDELPGLDLLVEPQLNLIREM
                     KRWGAEPALPPPGEGRRSDPDDPSYGWADVALLHAFLRKLRPRRVVCAGAGPFVPTLL
                     DLNEHVCGRSIAITVVDPEAERLRPILGREESEKVRLLPARVHEVPYDTFTSLGANDV
                     LCVETSHVSKTGSDVNHLLFRVVPRLKAGVHVHVDGVFWPFEYPSAWVTEGRAFNEAY
                     LWRAFLLNSSSWEIAFFASFLETSHRGALLKELPQWQRSRGGGLWLRRKG"
     gene            complement(2413..3189)
                     /locus_tag="PLP50_16565"
     CDS             complement(2413..3189)
                     /locus_tag="PLP50_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53206.1"
                     /translation="MPTISTRKFIALRKLTRALAETLRADVRSTLITIEPLLRPRAVF
                     GEHVQGHGKELVKGADRAFKDLVADFAEVAGSKPFHLPKELKSPFEVMSASLEMQPVE
                     YSYTARGEREGKTVRITSPLKWVVNYRDYGLDELKRVLADRNRTEEQLRRFVLHFLLV
                     EHVFTRQTGIREMLDALRLPVVVERRPEFGNLPITCVTTAVSTYRPDDEVIIETTEIS
                     GSEEFEEFVKVSDISGMKDALKEKLVELVRTQGPELLDEA"
     gene            complement(3290..4048)
                     /locus_tag="PLP50_16570"
     CDS             complement(3290..4048)
                     /locus_tag="PLP50_16570"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HPA53207.1"
                     /translation="MSKVPSAIASAALALVLTSCAPTARPPATAPAATAARASRPTDE
                     LHWVRGSAEYRAVTIQTFRAALEAAEEASAGRPAGSWAVCVDADDTIIDNSGYEAELQ
                     AAGRTHTDEAWVAWVKRRARPAVPGAAAFLAGVRALGGRVAVVTNTQASLCDDVAANL
                     VLAGLPYDVLVCRPDDAGDRKEPRFRLVTEGIARPDLGPLEILVWVGDNIQDFPDRSQ
                     KLRDEPESAFGEFGVRYFALPNPIYGTWEKNGPR"
     gene            4098..4529
                     /locus_tag="PLP50_16575"
     CDS             4098..4529
                     /locus_tag="PLP50_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53208.1"
                     /translation="MGKRIEPCRKSPEERLDDLLTGHREASLRNEGAKYVSRVIASSD
                     SMPNGVKFFAYALLAADADDEDEALEALASAERYLAVAREDLGRRFSREVASLRFLER
                     GIALRSERAEFEEALRLCDVALSLGLGEAYERKRASLLRMT"
     gene            complement(4567..4740)
                     /locus_tag="PLP50_16580"
     CDS             complement(4567..4740)
                     /locus_tag="PLP50_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53209.1"
                     /translation="MDFGIGLLIAAALAFGVYRFVVARTRREAERKTRADRKAKVARD
                     DAKAIQRAIDARR"
BASE COUNT          661 a         1720 c         1815 g          693 t
ORIGIN      
        1 ggacggtgtc ggtccccttc atcgtcagga gcggcatcag ccccttctcc gcgacggcct
       61 gcatcgcccg gacggtgaag agcgcctcgg cgcacggcac ggcgcgcttc tcgccgtcct
      121 cctccacgag cgcgaacggg aggccggaga tctcctgcgc cgacccgggg cggaggctcc
      181 agccgtcctc gaggaacgcc tccccgagaa gcagcgccgt cgcgagcgcc gggctgcccc
      241 agaggtactg ctcgtgctcc ggcggagagg agagctcctc gaagtcgaag gcgtcgacgg
      301 cgttcgtctc cttcccgtaa gggagcctga ggaggaggcg cggcagcgcg aggccgacga
      361 aacgcgcctc cggaagccgg cggagcgccg tccaggcctc ggccccctcg cccgccggct
      421 cggtccagtc gtcggggtcg ggcgtcgccg cgagcgaggc gcagccgacg agtcccgggt
      481 cggtcccggc gaggacggcc gtccctgcca gcgcggcgat ccgcgcgagg cgtccgagcg
      541 cctcggcgtc ctcgcgcgac ggcccgaagg agtcgagaag gacgaaggcg gcccagggct
      601 ctccgccggg agtccccacg gtcttctcga cgaggaggcg gtggaggccc gtccgcccga
      661 ggtcgtcgcc gacgaggagg tcggcgcgga gctcctcggg agagaggtcg acgaggtgga
      721 gcgtgagcgg gccgtcgacg tcgagccgcc ggacgaggaa gtcgacgccg cgccagctcg
      781 cctcgagcgc ccggaacgcc gcgctcccca ggatcgcccg catcagctcg gtcggcccgt
      841 cggggccggc ctcgacgacg ggcgtctcct cctgcggggt cccgtcgagg atgcggtcga
      901 ggagaccggc ggccgggcgc gccgtctccc gccggcgtga ctccgctgcg gcggcgtcct
      961 tcagcgcgcc gaagcgcgcg atgcgcgaga agagggtgtc cgggaggaag tcgtccagct
     1021 cgcggaagcg gaggacgtcg ccgtcgcgca ggcggacctg cggctcgagg cgggcgatcg
     1081 tcgcgtcgag cgagtcgagg tcgacgcgga acgcctttcg ctcgccgagg gtcgtcccgg
     1141 tctcgacgag accacgggcc cgccggcccg agaagtcccc ggcgaggagg atccggaacg
     1201 gggcgccgtc ctccgcgcgc gccgggcgcg gctcggagcc ggattcgaga cggacgtcga
     1261 tcgcaccgaa ggagaacggc ttcgacatct ggggcggcct cctcgacgtt tggtgggctc
     1321 gagtataggg gcgggcgcgc tagactcgtt ccctcacagc aaggaggtcc cgttgatccg
     1381 cttcctgaag aaggtggggc ggcagctgcc ggtcctgaag gagatccacc gttacatcct
     1441 cgctctggag gccgagcggg aggagctccg cggaaccgtc gcgacgcagc gggaggagct
     1501 ggtccgggcc cgggagacga cgcggaagct ctgggtcccg cccggcgacc cgcggtcccc
     1561 gatcccggac ctcgacgacg tgcgccggcg cgagagcgag ctcttcgggc gtcctcccga
     1621 cgagctgccc ggtctcgacc tgctcgtcga gccgcagctg aacctgattc gggagatgaa
     1681 gagatggggg gccgaaccgg cccttccgcc gccgggagag ggccggcgga gcgaccccga
     1741 cgacccctcg tacgggtggg ccgacgtcgc cctgctgcac gccttcctcc ggaagctccg
     1801 tccccggcgc gtcgtctgcg cgggcgccgg gcctttcgtc ccgaccctcc tcgacctgaa
     1861 cgagcacgtg tgcggtcggt cgatcgcgat caccgtcgtc gacccggagg ccgagcggct
     1921 ccgcccgatc ctcggtcggg aggagtcgga gaaggtccgg ctcctgccgg cccgcgtcca
     1981 cgaggtcccc tacgacacct tcacgtcgct cggcgccaac gacgtcctct gcgtcgagac
     2041 gagccacgtc tcgaagaccg ggagcgacgt gaaccacctc ctcttccgcg tggtcccgag
     2101 gctgaaggca ggggtccacg tccacgtcga cggggtcttc tggcccttcg agtacccgag
     2161 cgcgtgggtg acagagggac gcgccttcaa cgaggcgtac ctttggaggg cgttcctcct
     2221 caacagctcc tcctgggaga tcgcgttctt cgcgagcttc ctggaaacct cccaccgggg
     2281 ggcgctcctc aaggagctgc cgcagtggca gcggagccgc ggcggagggc tctggctgcg
     2341 tcgaaagggc tgaacgggag cgggggccgg aggggagcgg acgcctcgcg cggcgggagc
     2401 cgaccggagg cgttaggcct cgtccaggag ctccggcccc tgcgtccgga cgagctcgac
     2461 gagcttctcc ttcagggcgt ccttcatccc cgagatgtcg gagaccttca cgaactcctc
     2521 gaactcctcg ctccccgaga tctccgtcgt ctcgatgatc acctcgtcgt cgggacggta
     2581 cgtggagacg gccgtcgtga cgcaggtgat cgggaggttc ccgaactcgg ggcgccgctc
     2641 gaccacgacc gggagacgaa gcgcgtcgag catctcccgg atccccgtct gccgcgtgaa
     2701 gacgtgctcg acgaggagga agtggaggac gaagcgcctc agctgctcct cggtccggtt
     2761 gcggtcggcg aggacgcgct tgagctcgtc gaggccgtag tcgcggtagt tgacgaccca
     2821 cttcagcggc gaggtgatcc ggaccgtctt gccctcgcgc tctccgcggg cggtgtacga
     2881 atactcgacc ggctgcatct cgagcgaggc gctcatcacc tcgaacggcg acttcagctc
     2941 cttcgggagg tggaacggct tgctcccggc gacctccgcg aagtcggcca cgaggtcctt
     3001 gaaggcccgg tcggcgccct tcacgagctc cttcccgtgc ccctggacgt gctcgccgaa
     3061 gaccgcgcgc ggacggagca gcggctcgat cgtgatgagc gtgctgcgca cgtcggcgcg
     3121 aagcgtctcc gccagggcgc gggtcagctt tcgaagggcg atgaacttcc gcgtgctgat
     3181 cgtcggcatt cgcgtcccct ctcccggcgc gccgggccgc gccgcggctc tccgggtgat
     3241 cgtttcgtac gagtctggag ccgattcgac ggggcgtcaa accgcccgct cagcgggggc
     3301 cgttcttctc ccaggtcccg tagatcgggt tcggcagcgc gaagtagcgg acgccgaact
     3361 ccccgaacgc gctctcgggc tcgtcgcgca gcttctggct ccggtcgggg aagtcctgga
     3421 tgttgtcgcc gacccagacg aggatctcga gcggccccag gtcgggccgc gcgatcccct
     3481 cggtgacgag ccggaatcga ggctccttgc ggtcgcccgc gtcgtccggc cggcagacga
     3541 ggacgtcgta cggcaggccc gcgaggacga ggttcgccgc cacgtcgtcg cagagcgacg
     3601 cctgcgtgtt cgtcacgacg gcgacccggc cgccgagcgc gcggaccccg gcgaggaagg
     3661 cggcggcgcc ggggacggcc ggccgtgccc gcctcttcac ccaggcgacc cacgcctcgt
     3721 cggtgtgggt ccggcccgcg gcctgcagct cggcctcgta gccggagttg tcgatgatcg
     3781 tgtcgtcggc gtcgacgcag acggcccagc tcccggcggg acggccggcg ctggcttctt
     3841 ctgcggcctc cagcgcggcc cggaaggtct ggatcgtgac ggcccggtac tcggccgagc
     3901 cgcggaccca gtggagctcg tcggtggggc ggctcgctcg ggcggccgtc gcggccggtg
     3961 cggtggccgg cgggcgcgcc gtgggcgcgc aggacgtcag gacgagagcg agtgcggcgg
     4021 aggcgatggc ggacgggacc ttgctcatcg gaattcctga gtctagcagc gcccgcgctc
     4081 gcgtactctc acgtcccatg ggaaaacgga tcgagccgtg ccggaagagc cccgaggagc
     4141 gcctcgacga cctcctcacg gggcatcgcg aggcgtcgct gcggaacgag ggggcgaagt
     4201 acgtctcgcg tgtcatcgcg tcgtccgact cgatgccgaa cggcgtgaag ttcttcgcgt
     4261 acgccctcct ggcggcggac gccgacgacg aggacgaggc gctggaggcg ctggcgtccg
     4321 cggagagata cctcgcggtg gcacgcgagg acctcgggcg ccggttctcg agggaggtcg
     4381 cctcgctccg cttcctcgag cgcggcatcg ccctccggag cgagagagcc gagttcgagg
     4441 aggcgctccg cctctgcgac gtggcgctct ctctcggcct cggggaggcc tacgagagga
     4501 agagggcgtc tcttctccgg atgacgtagg gacgggggcc cgccacgagg cggcgggcgc
     4561 tcccgatcag cgccgggcgt cgatggcccg ctggatcgcc ttcgcgtcgt cccgggcgac
     4621 cttcgccttg cggtcggccc gcgtcttccg ttccgcttcg cggcgggtgc gtgcgacgac
     4681 gaagcggtag acgccgaagg cgagcgccgc ggcgatcagg agcccgattc cgaaatccat
     4741 ctctggcgct ctcctcggct cccgcggccg gcccgcggag gcccgggtga cccgggcccc
     4801 cgcggacgac gaccggggcc ggacgtgacg tccgggacta ccagcggccg cgcccgccgc
     4861 cgccgccgcg gttctcctgc ggcttggcc
//
LOCUS       DAOONL010000200         4836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_21107_length_4836_cov_19.417068, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000200 DAOONL010000000
VERSION     DAOONL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21107_length_4836_cov_19.417068"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PLP50_16585"
     CDS             complement(<1..1444)
                     /locus_tag="PLP50_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPA53210.1"
                     /translation="MNITEVCIRKPVLAWMLMAATIVFGGVALSRIGISQFPDVDSPT
                     INVSVTWEGAAPEVMEHDVVEVLEEAVMQVEGVKSLTSSSRQGSANITIELDINRNID
                     LALQDTQTKVSQAQRRLPRDIDPPVVSKSNPEDNPILWMGLSGPFPRQVLADYARYRL
                     KERFQTVPGVGEVTMGGYLDRNVRIWVDADKLNAKGMTVTDVIAALQREHVELPAGRL
                     ETEGREISVRVMGEAVDLTTLRKISLRDLNGAPVYLEDVALVEDGFEDERRIARVNGA
                     PAQGLGIRKQRGSNAVAVAQGVKAAMAEIQKTLPPGMEVGINFDSTTFIEDSVHEIQF
                     EILLSVVLTGLVCWMFLGSLSSTLNVVLAIPMSLLGTVAFIYFAGFTLNTFTLLALGL
                     AVGIVVDDAIMVMENIFRHFEEGKDRVTAAREGTGEITFAALSATIAIIAIFIPVVFM
                     KGVIGKFFLQFGVTLCVAVALSYLEAITLAP"
     gene            complement(1441..2592)
                     /locus_tag="PLP50_16590"
     CDS             complement(1441..2592)
                     /locus_tag="PLP50_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPA53211.1"
                     /translation="MKRRTLAPVVLLAGAAALVLSCSGGGEKKGPSGKGGPPAFPVET
                     TKVATRTVEYSVSAVGSVEAFEKVQVTARVAGVVEQVKFVEGESVEAGTPLVEIEPRR
                     YQLAVEAARANVQKAEAAKADAEAALARRKAVTEKNPGLIPGEEIATFATRVQTAAAD
                     VAQARSALDQAELNLRDAFVRAPVAGRIETRTVTTGQYVSPGGVLATLVRRDPLLLRF
                     QVPSEDARNLRTGLVARFRVPGDEGEFRARITHVAGAAETDSRMVAVTAQVDDPRRAA
                     LRPGAFADVTVPVGGVARAAVIPQTAIRPSERGFLAFVVVDGKARERVLTLGLRTTEG
                     LVEVKAGLAAGEELVVRGAEALREGLPVAVRAPGETPKDDAPARREARG"
     gene            complement(2589..3884)
                     /locus_tag="PLP50_16595"
     CDS             complement(2589..3884)
                     /locus_tag="PLP50_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA53212.1"
                     /translation="MPLLLLPFLLSTLALADGAPLTLDEAIRLAVSRNERALASGERL
                     EAAEARVAKARAFFFPDVQAAGSYTRRPYERTSTVGGQEIVVSKHDAFSTSITARQVL
                     FDARALPLWRQARSDRDAASLEAADERRLVAFEAAAAFLRTLGADQVAGAAERRVELA
                     RTNLEDARARFEAEITSSNDVTRAELELATAEREATRARNDEETSRLSLANLLVAEVP
                     EELKVPQPLLASAERERPAEEELLTRARERRLDVAAGKKRAEALRFYAKEPMNRFFPS
                     LVARADGRWTNEGGLNARNTTWTYGLDLSWSLFDSATRFADRRERDANARAADLNARA
                     RERGVELEVKSALASLDNARAAVRQAAVALEAARKNAAETTTLYREGLANALAVADAN
                     QSLFEADVALTNERYGLGAAVLDTYAALGLDPEGKEPLP"
     gene            complement(3987..4625)
                     /locus_tag="PLP50_16600"
     CDS             complement(3987..4625)
                     /locus_tag="PLP50_16600"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HPA53213.1"
                     /translation="MRHLTSAALATLLLAAPAALAQDSLRFGTPSGSGPYTVPILLAD
                     DAGTPLGVDRPAGSRIQAFAVTVRFTPASAVTSASLARAGLLAGSTPVFETTAEGDGT
                     ITWVGSFDEAGGALPFSQPPGAGDPILALSVTLASGATVSASLDGATTTLSNQAGTAS
                     ETVAGGTLALGPPVGLPTGPEDAAAVPTLGEGALLALAAAIAAAGLRLVRRA"
     gene            complement(4640..>4836)
                     /locus_tag="PLP50_16605"
     CDS             complement(4640..>4836)
                     /locus_tag="PLP50_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPA53214.1"
                     /translation="IGLPGDANDDGVVNPADVFYLVAFLYSGGPAPLGVADANFDERV
                     DALDLFYLINFLYAGGPEPQ"
BASE COUNT          658 a         1606 c         1892 g          680 t
ORIGIN      
        1 ccggcgcgag cgtgatcgcc tcgaggtagg agagggcgac ggcgacgcag agcgtcacgc
       61 cgaactggag gaagaacttc ccgatgaccc ccttcatgaa gacgaccggg atgaagatcg
      121 cgatgatcgc gatcgtcgcc gagagggccg cgaaggtgat ctcgcccgtc ccctcgcggg
      181 ccgccgtcac ccggtccttc ccctcctcga agtgccggaa gatgttctcc atgaccatga
      241 tcgcgtcgtc gacgacgatc ccgacggcca ggcccagagc gaggagcgtg aaggtattca
      301 gcgtgaagcc ggcgaagtag atgaacgcga ccgtcccgag gagcgacatc gggatcgcga
      361 ggacgacgtt cagcgtcgag gagagggagc cgaggaacat ccagcagacg agccccgtca
      421 ggacgacgga gaggaggatc tcgaactgga tctcgtggac ggagtcctcg atgaacgtcg
      481 tcgagtcgaa gttgatcccg acctccatcc ccggcgggag ggtcttctgg atctcggcca
      541 tcgcggcctt gacgccctgc gcgacggcga cggcgttcga gccgcgctgc ttgcggatgc
      601 cgagcccctg ggcaggggct ccgttcacgc gggcgatgcg ccgctcgtcc tcgaagccgt
      661 cctcgacgag ggcgacgtcc tcgaggtaga ccggcgcgcc gttcaggtca cggagggaga
      721 tcttgcggag ggtcgtcagg tcgaccgcct cgcccatgac gcggacgctg atctcgcgcc
      781 cctcggtctc cagccggccg gccggaagct cgacgtgctc gcgctggagc gcggcgatga
      841 cgtctgtgac cgtcatcccc ttcgcgttca gcttgtcggc gtcgacccag atcctcacgt
      901 tgcggtcgag gtagcccccc atcgtgacct cgccgacgcc gggaacggtc tgaaagcgct
      961 ccttgaggcg gtagcgggcg tagtcggcga ggacctgccg cgggaacggc ccggagaggc
     1021 ccatccagag gatcgggttg tcctcggggt tcgacttcga gacgacgggg gggtcgatgt
     1081 cgcggggcag gcgccgctgg gcctgggaga ccttcgtctg ggtgtcctgg agcgcgaggt
     1141 cgatgttccg gttgatgtcg agctcgatcg tgatgttcgc ggagccctgc cgggaggagg
     1201 aggtgagcga cttcaccccc tcgacctgca tcacggcctc ctccagaacc tcgacgacgt
     1261 cgtgctccat gacctcaggg gcggccccct cccaggtgac ggagacgttg atcgtcggcg
     1321 agtcgacgtc ggggaactgg ctgatgccga tgcgcgagag cgcgacgcct ccgaagacga
     1381 tcgtcgcggc catcagcatc caggccagga ccggcttccg gatgcagacc tcggtgatgt
     1441 tcatccgcgg gcctcccggc gggcgggcgc gtcgtccttc ggcgtctccc cgggcgccct
     1501 caccgcgacg gggagcccct cgcggagcgc ctcggcgcct cggacgacga gctcctcgcc
     1561 ggccgcgagc cccgccttca cctcgacgag cccttccgtc gtgcggaggc cgagcgtcag
     1621 gacgcgctcc cgggccttcc cgtccaccac gacgaacgcg aggaacccgc gctcgctcgg
     1681 ccggatggcc gtctgcggga tgacggccgc gcgggcgacg cccccgacgg ggacggtgac
     1741 gtcggcgaag gcgccgggac ggagggccgc gcggcgcggg tcgtcgacct gcgcggtgac
     1801 ggcgaccatc ctcgagtcgg tctccgccgc tccggcgacg tgggtgatcc gggcccggaa
     1861 ctccccctcg tcgcccggga cgcggaagcg ggcgacgagc ccggtccgga ggttccgggc
     1921 gtcctcggac gggacctgga agcggaggag gagcgggtcg cggcggacga gcgtcgcgag
     1981 gacgccgccg ggggagacgt actgtccggt cgtcacggtg cgggtctcga tccgcccggc
     2041 gacgggagcg cggacgaacg cgtcgcgcag gttcagctcg gcctggtcga gggcggaccg
     2101 ggcctgggcg acgtcggccg cggcggtctg gacgcgggtc gcgaaggttg cgatctcctc
     2161 gccgggaatg agcccggggt tcttctccgt gacggctttc cggcgcgcga gcgcggcctc
     2221 ggcgtcggcc ttcgcggcct cggccttctg gacattcgcg cgggccgcct cgacggcgag
     2281 ctggtagcgg cgcggctcga tctcgacgag cggcgtcccg gcctccacgc tctcgccctc
     2341 gacgaacttg acctgctcga cgaccccggc gacgcgggcg gtcacctgga ccttctcgaa
     2401 ggcctccacg gagccgacgg cgctcacgga gtactcgacg gtccgggtcg cgaccttcgt
     2461 cgtctcgacg gggaaggccg gcgggccgcc ctttcccgat ggtcccttct tctcgccgcc
     2521 tccggagcag gagaggacga gcgcggcggc tccggccagg aggacgacgg gggcgagggt
     2581 gcggcgcttc acgggagggg ctccttcccc tctgggtcga gcccgagggc ggcgtaggtg
     2641 tcgaggacgg cggctccgag accgtagcgc tcgttcgtga gggcgacgtc ggcctcgaag
     2701 agggactggt tcgcgtcggc gacggcgagg gcgttcgcga ggccttcgcg gtaaagcgtc
     2761 gtcgtctcgg cggcgttctt gcgcgccgcc tcgagcgcga cggcggcctg acggacggcg
     2821 gcccgggcgt tgtcgaggga ggcgagggcg ctcttcacct cgagctcgac gccgcgctcg
     2881 cgggcgcggg cgttcaggtc ggccgcgcgg gcgttcgcgt ctcgctcacg tcggtccgcg
     2941 aagcgggtcg cggagtcgaa gagcgaccag gagaggtcga ggccataggt ccaggtcgtg
     3001 ttccgggcgt tcagtccccc ctcgttcgtc caccggccgt cggcgcgcgc gacgagggac
     3061 gggaagaagc ggttcatcgg ctccttcgcg tagaagcgga gcgcctcggc gcgcttcttc
     3121 ccggcggcga cgtccagccg gcgctcgcgg gcccgcgtga ggagctcctc ctccgccggg
     3181 cgttcgcgtt cggcggaggc gaggaggggc tgcgggacct tgagctcctc gggcacctcg
     3241 gcgacgagga ggttcgcgag cgagagccgg gacgtctcct cgtcgttccg cgcccgggtc
     3301 gcctcccgct cggcggtcgc gagctccagc tcggcgcggg tcacgtcgtt gctgctcgtg
     3361 atctccgctt cgaaccgggc gcgcgcgtcc tcgaggttcg tccgggcgag ctcgacgcgg
     3421 cgctcggcgg cgccggcgac ctggtcggcg ccgagcgtcc ggaggaaggc cgcggccgcc
     3481 tcgaacgcca cgaggcgtcg ctcgtcggcg gcctcgaggc tcgcggcgtc ccggtcgctt
     3541 cgcgcctgcc tccagagcgg gagcgcgcgg gcgtcgaaga ggacctgtcg tgcggtgatg
     3601 ctcgtcgaga aggcgtcgtg cttggagacg acgatctcct gcccgccgac cgtgctcgtc
     3661 cgctcgtagg gacgccgcgt gtagctcccg gcggcctgca cgtcggggaa gaagaacgcg
     3721 cgcgcctttg cgacgcgcgc ctccgccgcc tcgaggcgct cgccggaggc gagggcgcgt
     3781 tcgtttcgcg agacagcgag acggatcgcc tcgtcgaggg tgagaggtgc accgtcggcg
     3841 agggccaggg tcgagaggag gaaggggaga aggagaaggg gcaccggagg gatacgtgcc
     3901 ccgcggaggg ggagatgcat accgcggggg atagtaccgg acggccgttc ggtttttccg
     3961 tcgaaacgcg gacgtgtggc gaagtgtcac gccctccgga cgaggcggag gcccgccgcg
     4021 gcaatcgccg cggccagcgc caggagagcg ccttctccga gggtcgggac ggcggcggcg
     4081 tcttcgggac cggtgggaag cccgacgggc gggccgaggg cgagcgtccc gccggccacg
     4141 gtctcgctcg ccgtcccggc ctggttcgag agcgtcgtcg tggcgccgtc gaggctcgcg
     4201 ctcacggtcg ctccggacgc gagcgtcacc gagagcgcga ggatcggatc gccggcgccg
     4261 ggcggctggg agaagggaag cgcgccgccg gcttcgtcga acgacccgac ccacgtgatc
     4321 gtgccgtcgc cctcggccgt cgtctcgaag accggagtcg accccgcgag gaggcccgcg
     4381 cgggcgaggc tcgccgaggt gacggccgag gccggggtga agcggacggt gacggcgaag
     4441 gcctggatcc gcgagccggc cgggcggtcg acgccgaggg gcgtgccggc gtcgtcggcg
     4501 aggaggatcg gaacggtgta cgggccggag cccgaggggg tcccgaagcg gagcgagtcc
     4561 tgcgcgaggg cggccggggc ggccaggaga agcgtggcga gggcggcgga ggtgagatgg
     4621 cgcatcgtcg actccttgct cactgcggct ccgggccgcc ggcgtagagg aagttgatga
     4681 ggtagaagag gtcgagggcg tcgacgcgtt cgtcgaagtt cgcgtcggcg acgccgagcg
     4741 gcgcggggcc gccggagtag aggaaggcga cgaggtagaa gacgtcggcg gggttcacga
     4801 cgccgtcgtc gttcgcgtcg cccggcaggc cgatca
//
LOCUS       DAOONL010000201         4820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_21196_length_4820_cov_9.458342, whole genome shotgun sequence.
ACCESSION   DAOONL010000201 DAOONL010000000
VERSION     DAOONL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21196_length_4820_cov_9.458342"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLP50_16610"
     CDS             <1..508
                     /locus_tag="PLP50_16610"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HPA53215.1"
                     /translation="VSDDFERALEFTLRWEGGKVDDPHDHGGRTNRGITQRTYDGWRS
                     DHDLPARDVYLLEEDELQDIYRTLYWWVAKGEEWPASLVTFDTAVLFGASRAAEWLQA
                     ASWADVAPAKRAWAILCLRRERHRDRVAKDRTQARFWAGWMNRLNDLARFAGLVPGSM
                     GGAQAPNS"
     gene            1018..1191
                     /locus_tag="PLP50_16615"
     CDS             1018..1191
                     /locus_tag="PLP50_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53216.1"
                     /translation="MTDRIVNLLRPRVKPLPDPAHERAYVTARGTAIRLGDHVREMNE
                     WRDSYSFRTGRAA"
     gene            1191..1400
                     /locus_tag="PLP50_16620"
     CDS             1191..1400
                     /locus_tag="PLP50_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53217.1"
                     /translation="MHLTRWTKSLLSERARRHEAARWDAEAEVSALEAEIRDLRAKRF
                     NAEVKARRHCRLAYTLRGLRDGGTA"
     gene            1492..2460
                     /locus_tag="PLP50_16625"
     CDS             1492..2460
                     /locus_tag="PLP50_16625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HPA53218.1"
                     /translation="MEGTNRITLAEPSREEWLEERKTGLGGSDAAAVLGLNPWASPLS
                     VYLEKLGLAEPKEESRPMRLGKRMEPVILEEYLRETGGRASLNARVYRHPEFPEILGT
                     PDAFREDEPVGVELKWIGDRSASQWGESGTDDIPDYYYTQVAVYMAVTNREAWDLAAI
                     IGGRDLRIYRIERNLALEEELLGRLRAFWRDHVLAQVPPEVDGSADARRWIDARFPRQ
                     RAALRPATAQEEAWARELAVVREAKVRIEGDEERLRNLLTASIGEAEGIEGAFGRITW
                     KAGKDSVRTDWKGLVAALNPTAEIVSRFVSIEPASRRFLPSFKKEN"
     gene            2462..3334
                     /gene="recT"
                     /locus_tag="PLP50_16630"
     CDS             2462..3334
                     /gene="recT"
                     /locus_tag="PLP50_16630"
                     /inference="COORDINATES: protein motif:HMM:NF007351.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HPA53219.1"
                     /translation="MAQEALARTSSAAITPKARMETLRSLLEAAKPSMSQVVPKHVTP
                     ERLVKLALVAASRTPLLLQCEPKSIVQGVMTAAQLGLDCGGVLGSAYLVPFKNAKNGG
                     RYEAQLIVGYRGLVDLARRSGEIETIEAHVVYAHDSFEIAYGLDPVLRHVPCLTGDAG
                     DPILVYAIARLKDGGHQLEVMSRSEVERIRSRSRAGSSGPWVTDWAEMARKTVVRRLC
                     KYLPLSPELADALVADEKVEGSASVEIVPVEEPVGEEEPLESEVVAPAEGKAAGLLAR
                     IKENTEGREPGVEG"
     gene            3338..3640
                     /locus_tag="PLP50_16635"
     CDS             3338..3640
                     /locus_tag="PLP50_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53220.1"
                     /translation="MHYVINVSVLREGKTTFRARAKLSQKGQTIAVGDRKAQSVARAV
                     GESLELVWDRLVPVAVVDARDALAGRRRLAPADPVEEEPVGVLPEEVSSLFPGDAA"
     gene            3637..3876
                     /locus_tag="PLP50_16640"
     CDS             3637..3876
                     /locus_tag="PLP50_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53221.1"
                     /translation="MNTYEGLRVENARLADGYLEALDRLGAIRRLQVALKASQWEDEV
                     GVAAILETILDAPGDDLKNLPGAFWTEPLDVRRVS"
     gene            4072..4317
                     /locus_tag="PLP50_16645"
     CDS             4072..4317
                     /locus_tag="PLP50_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA53222.1"
                     /translation="MHAANVAKSARLQRVLEALRAHPEGLTTWEITEETGTLAVSATV
                     AELRQNLAPLGQDVRCEYVSRTRHGARIYRYTLEGAA"
     gene            4314..>4820
                     /locus_tag="PLP50_16650"
     CDS             4314..>4820
                     /locus_tag="PLP50_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53223.1"
                     /translation="MSHLYGKVFGSLYEGSMLGAGVHVFALWPYCLARADARGVVEVN
                     PKLAAVVLGCSVTEIEQALEYLQRPDPDSRSREEEGRRLVREGQFAYRIVNYLRYREI
                     RDAEARREYQREWVKGKRANRRKSTKVDCRQVSTKSTKGEGEGEGEGKEEGESSAPAR
                     RPSPKTLLE"
BASE COUNT          794 a         1586 c         1784 g          656 t
ORIGIN      
        1 cgtgagcgac gacttcgagc gggcgctgga gttcaccctc cgctgggagg gcgggaaggt
       61 cgacgacccg cacgaccacg gcggcaggac gaaccggggc atcacgcaac ggacctacga
      121 cgggtggcgc tccgaccacg acctccccgc gcgggacgtc taccttctcg aggaggacga
      181 gctccaggac atctatcgga ccctctattg gtgggtggcg aagggcgagg agtggccggc
      241 gagcctcgtc acgtttgaca ccgcggtcct cttcggcgcc tcccgggcgg cggagtggct
      301 ccaggcagcg tcctgggccg acgtcgcccc ggcgaagcgc gcctgggcga tcctctgcct
      361 ccggcgtgag aggcaccggg accgagtggc gaaggaccgg acgcaggcgc ggttctgggc
      421 cggctggatg aaccggctga acgacctcgc ccgcttcgcc ggcctcgtgc cgggaagcat
      481 ggggggagcg caggcgccta actcctgacg ggtcagcgcc cggggagact tctacggtgt
      541 gctccggccg gccggcatcg tggttctcgc cgtagggaat gggcgggggg tcgccgggct
      601 tcatcccgag ggcagcgtct accagccgct tgaacgtggc gttccgcgat aaccgctccc
      661 gccctgcaag tgcatctacg gcagcgactt ggtccgcgtc gaggcggatg ctgacgtgcg
      721 ggaggggctc gcggttcttc atgtagtcaa gggtagtcaa agaaagtact tgacgaaatg
      781 agtcgtgatg actacattct cttgcatgga gaagccgaaa cgactacagc accccacctc
      841 gatccgactc gcgcccgcgc aggtcgaggc ggtccgggaa atcgcacagc gggagcagcg
      901 gtcgattggg ggcgcgcttc gcgtcctcgt ctcggaagcc atagccgccc gggcacgccg
      961 gacgaggagg gccaacgaag gcccccgcgc caacgggggc cagaagggag accgaaagtg
     1021 accgaccgaa tcgtgaacct gttgaggccg cgggtcaagc cgctgccgga tccggcgcac
     1081 gagagggcct acgtgaccgc tcgtggcacg gcgatccgcc tcggggacca cgtccgcgag
     1141 atgaacgagt ggagggacag ctactcgttc cggacgggga gggcggcgtg atgcacctga
     1201 cccgctggac gaagtcgctc ctttccgagc gcgcccgccg gcacgaggcg gcgcggtggg
     1261 acgccgaggc cgaggtctct gcgctcgagg cagagatccg ggatctgcgg gcgaagcgct
     1321 tcaacgccga ggtcaaggcg cggcgccact gccggctcgc ctacaccctc cgcgggctgc
     1381 gggacggggg gaccgcgtga tgcccgactc cgtttggctc ctcgtcgtcg gggtggtcgt
     1441 cctggtctgg atcgggagga gcggccggaa ggccgcgtag gagaagaagg catggaaggc
     1501 accaacagaa tcaccctcgc ggaaccctcg cgggaggagt ggctcgagga gcggaagacg
     1561 ggcctcggtg gctcggacgc tgcggcggtc ctcgggctga acccgtgggc gtcgccgctc
     1621 tcggtctacc tcgagaagct cggcctcgcc gagccgaaag aggagagccg gccgatgcgc
     1681 ctcgggaaac gcatggagcc ggtcatcctc gaggagtacc tccgagagac gggcgggcgg
     1741 gcgagcctaa acgcccgcgt ctaccggcac ccggagttcc cggagatcct cgggaccccc
     1801 gacgccttcc gcgaggacga gccggtgggc gtcgaactga agtggatcgg cgaccgctcg
     1861 gcgtctcagt ggggcgagag cgggaccgac gacatccccg actactacta cacccaggtc
     1921 gccgtctaca tggcggtgac gaaccgcgaa gcgtgggacc tcgcggcgat catcggaggg
     1981 cgcgatctcc ggatctaccg gatcgagcgg aacctcgcgc tcgaggagga gctcctcggc
     2041 cggctccgcg cgttctggcg cgatcacgtc ctcgcccagg tcccgccgga ggtggatggg
     2101 tcggccgacg cccggcgctg gatcgacgcg cgattcccgc ggcagcgtgc cgcgctccgg
     2161 ccggcgacgg cgcaggaaga ggcgtgggcg cgcgagctcg ccgtcgtccg cgaggccaag
     2221 gtccgcatcg agggcgacga ggagcgcctc cggaacctcc tgacggcgtc catcggtgag
     2281 gccgagggga tcgagggggc gttcggccgg atcacctgga aggccggcaa ggactccgtc
     2341 cgtaccgact ggaagggtct cgtcgccgcc ctgaacccca cggcagaaat cgtgtcccgc
     2401 ttcgtttcca tcgagccggc ctcgcgccgg ttcctcccca gcttcaagaa ggagaactga
     2461 catggcgcag gaagcactcg cccggacgtc gtccgcggcg atcaccccga aggcacgcat
     2521 ggagaccctt cgctcgctcc tcgaggcggc gaagccgtcc atgagtcagg tcgtcccgaa
     2581 gcacgtcacc ccggagcggc tcgtgaaact cgcgctcgtc gcggcctcga ggaccccgct
     2641 cctgctccag tgtgagccga agtcgatcgt ccagggcgtg atgacggctg cgcagctcgg
     2701 actcgactgc ggcggcgtcc tcgggtcggc gtacctcgtt ccgtttaaga acgcgaagaa
     2761 cggcgggcgg tacgaggccc agctcatcgt cggctaccgc ggcctcgtcg acctcgcgcg
     2821 gcgctccggc gagatcgaga ccatcgaggc gcacgtcgtc tacgcgcacg attcgttcga
     2881 gatcgcctac gggctggacc cggtcctccg gcacgtcccc tgcctgaccg gagacgccgg
     2941 cgacccgatc ctcgtctacg cgatcgcgcg cctgaaggac ggcgggcatc agctcgaggt
     3001 gatgagccgc tcggaggtcg agcgcatccg ctcccgctcg cgcgccggct cctcggggcc
     3061 ctgggtgacg gactgggccg agatggcacg gaagaccgtc gtccgtcggc tctgcaagta
     3121 cctcccgctc tcgccggagc tcgcggacgc gctcgtggcg gacgagaagg tcgaggggtc
     3181 ggcctccgtc gagatcgtgc ccgtggagga gcccgtgggc gaggaggagc cgctcgagag
     3241 cgaggtggtg gcgccggccg aaggaaaggc ggcgggcctc ctcgcgagga tcaaggagaa
     3301 caccgagggg cgcgagcccg gggtcgaggg gtagccgatg cactacgtca tcaacgtctc
     3361 cgtcctccgc gaggggaaga ccacgttccg cgcgagggcc aagctctcgc agaagggcca
     3421 gacgatcgcc gtcggcgaca ggaaggcgca gagcgtcgcc cgcgccgtcg gggagtccct
     3481 cgagctcgtc tgggaccggc tcgtcccggt cgccgtggtg gacgctcggg atgcgctggc
     3541 cgggcgccgg aggctcgcgc cggcggaccc cgtcgaggag gagccagttg gcgtcctccc
     3601 cgaggaggtc tcctcgctct tcccgggaga cgccgcgtga acacgtacga aggcctccgg
     3661 gtggagaacg cgcggctggc cgacggctat ctcgaggcgc tggatcgtct cggggccatc
     3721 cggcgcctcc aggtcgccct caaggcgagc cagtgggagg acgaggtcgg ggtcgcggcc
     3781 atcctcgaga cgatcttaga cgcccccggc gacgacttga agaacctccc cggggcgttc
     3841 tggaccgagc cgctggacgt gaggcgggtc tcgtgacggg gcggctgaac ccccgtcacc
     3901 cgcacccgcc gtgcgtctgc acggactgcg gcgggacgat ccggaagggg gatgccgggg
     3961 ctgacccgac cttcgggtgc tgtccgaact gccggcggcg gcggggcggg gagcgcaacc
     4021 gcgagaaggt cgcccggcac ggcccccgga tcttcgacag gaggagcccc gatgcacgcg
     4081 gcgaacgtgg ctaagagcgc gcggctccag cgggttctcg aggcgctccg ggctcacccg
     4141 gagggcctga cgacctggga gatcaccgag gagaccggga ctctcgccgt ctccgcgacg
     4201 gttgcggagc tccggcagaa cctcgcgcct ctcgggcagg acgtccggtg cgagtacgtc
     4261 tcccgaaccc gccacggggc gcggatctac cgctacacgc tcgagggggc ggcatgagcc
     4321 atctctacgg gaaggtcttc gggagcctgt acgagggctc gatgctcggg gcgggcgtcc
     4381 acgtcttcgc cctctggccg tattgcctcg cgcgggccga cgcgcgggga gtcgtcgagg
     4441 tgaaccccaa gctagcggcg gttgtgctcg gctgctccgt gacggagatc gagcaggcgc
     4501 tcgaatacct ccagcgcccc gaccccgact cgcgctcccg ggaggaagag gggcgtcggc
     4561 tggtgcgcga ggggcagttc gcatatcgga tcgtgaacta cctgcgctac agggagatcc
     4621 gggacgccga ggcgaggcgc gagtaccagc gggaatgggt caaggggaag agggcgaatc
     4681 gacggaagtc gacaaaagtc gactgtcgac aagtgtcgac aaagtcgacc aaaggagaag
     4741 gagaaggaga aggagaagga aaagaagagg gagagtcatc ggcgcctgcg cgccgaccct
     4801 cccccaagac cctcctcgag
//
LOCUS       DAOONL010000202         4783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_21440_length_4783_cov_16.364636, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000202 DAOONL010000000
VERSION     DAOONL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21440_length_4783_cov_16.364636"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..615
                     /locus_tag="PLP50_16655"
     CDS             421..615
                     /locus_tag="PLP50_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53224.1"
                     /translation="MKYFDAPLAVALATAPNLTWKLGLSPDELLPAMLAAVFNALEAH
                     GPALQRVSTGALAPEAVLES"
     gene            752..1237
                     /gene="brxF"
                     /locus_tag="PLP50_16660"
     CDS             752..1237
                     /gene="brxF"
                     /locus_tag="PLP50_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HPA53225.1"
                     /translation="MQIGTADILARGVREAASRYYRLVLLVGMAGAGKSVALGALGSN
                     PTPRLSLGSALGARLLELSRRQRALDCARLLESILDETGADPVALDNLEILFDPALQQ
                     DVLRLLQGLSRNRTIVAAWPGEYVDGVLSHAESGHPEFVRYTNPEAVLVNLPPPGSHT
                     P"
     gene            1251..>4783
                     /locus_tag="PLP50_16665"
     CDS             1251..>4783
                     /locus_tag="PLP50_16665"
                     /inference="COORDINATES: protein motif:HMM:NF039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HPA53226.1"
                     /translation="MRYSELIHFEPIESVVQLRRADKSDEAKNLVRAFVISDRMAEQL
                     TDLVFPELQFEVPHDNKGVLVVGNYGTGKSHLMAVISAVAEDAATAKLLTNAKVADAA
                     KPIAGKFKVLRAEINTRMSLRDFVCNTLDAKLGELGVDYRFPSEQKVLNPKDAFAEMM
                     AAFHEKYPKHGLLFVLDELLDYLRSRKQLELIQDLNFLRAVGEFCSGSRFRFVGGVQE
                     SLFDSPSFQFVAETVRRVKDRFEQVRIAREDVAFVVSQRLLKKDAKQEARIREHLLKF
                     APLYGSMNERMDDFVRLFPVHPAYLSTFEGVRLAEKREVLKTISAEIRRLVDQTVPAD
                     APGLVAYDSYWKTLKDNPSFRAVEDIRKVVDKADVLEGRIQQSFTRPQYKPVALRIVN
                     ALAVHRLTTGDIYAKLGATAEELRDDLCLLMPIPEREAEFLRSVVETVLKEILKTVSG
                     QFLSFNKENGQYYLDIQKDVDFDSLIEKRAEGLAPDRLDHYYFEALARVLECVDQTYR
                     SGFRIWEHELEWLERKAGRSGYLFFGAPNERPNTTPPRDFYLYFIEPYDPVYFKDEKK
                     ADEVIFRLKDRPEGFEGPLKLFAGAREQAAAASGANRKIYDEKATEHLKLLTSWLAAN
                     APTHLEVTHQGRTKLLADVVRGKAPASFDRSSFRDTANLAASLLLASHFADRAPEYPT
                     FTMLVTRASRVQAATDALKWIGGGGKTKQGTAVLDALELLDGDVLRPRESRYGKNVLE
                     AFSQKSANQVLNRNELIREDGAIEYWFPFRVEPELLGVVLAALVHAGDVVISLPGRKL
                     DASGLDALLKIPADELAAFKHLERPKDLPLAPLQELFDLLSLPRGLIVNPANREDGVQ
                     KLHVAAAQRIEQAIAALQSLDSGLSFWGRPVVPEKERTAWASQIRDLKSFLESLQPYN
                     TVGKLKNFPHDVAAIQARRSALATVESVSTLAALCQQLSPLTAYLQTAEAILPVEHPW
                     VTTVRAAKDELVGRLATPKGRVEASLRRDVEVRLAELKAEYQAAYLKLHGQARLGAEA
                     DKKKVALLRDPRLAQLQKLASIEILPSHQLQEIQNRLLGLKTCLSVAQQELDSAPHCP
                     HCGFRPAEEAISAAPGGVLGEVDERLDKLVHDWTTTLLDNLSDPTISGNIELVTDPKG
                     KQALQAFLRKKNLPEPVDSHLVKALQEVFSGL"
BASE COUNT          823 a         1697 c         1507 g          756 t
ORIGIN      
        1 gggcagctcg cggccgtccc cgttctcgat gagcgcgacg agcgtttcga ggacgccctt
       61 gtaggtgtcc ttcaggacac gggcggcctc accgtcacgg ccgctggccc agttgagcat
      121 cgggacctcc gttcctccgc gaagggcgga gtcgccgtga tggttggacc ggatggggga
      181 cagatggagt ccgggtgtga tccaggactg gcagcacctc gcctgtcggc tccggcgcca
      241 gctcggggcc aggtcgaggc caaccccgct cgaggaaacg cggcttctcg gagaactggt
      301 gctctacgaa aagtttcccc gccttcccca cggacaccat ctgtccgggt atcgggggga
      361 gcttgatcgg cgtgcgcgct cgacatccaa cccgggcggt cggcccgcga aggaggggag
      421 atgaagtact tcgacgcccc cctcgccgtt gcgctcgcca ccgccccgaa cctcacctgg
      481 aaactcggtc tcagcccgga cgagctcctt ccggcgatgt tggcggcggt cttcaatgcg
      541 ctcgaggcgc atgggccggc gctgcagagg gtctcgaccg gggcgctggc ccccgaggcc
      601 gtgctcgagt cgtgaccgag aaccgaaccc gcgtaggaag tctcggaagc cggggcggct
      661 gattctcgtc cgggctggac ggaatgggct gcgggcggcc agactctggc tccacgggat
      721 tcgccacgag gtcaaacggg aggcctaacg gttgcagatc ggcacggcag acattctcgc
      781 gcggggagtg agggaggcgg cgagccgcta ctaccgactc gtcctgctcg tcggcatggc
      841 cggggcggga aagtcggttg ctctcggggc cctcgggtcc aaccccacgc ctcgcctttc
      901 cctcggctct gccctcggcg cccgcctcct cgagctgagc cggcgccagc gggccctgga
      961 ctgcgccagg ctcctcgagt cgatcctcga cgagaccggg gccgaccccg tcgccctcga
     1021 caatctcgaa atcctcttcg atccggccct tcagcaggac gtcctccgtc tgctccaggg
     1081 cctttcccgg aaccggacga tcgtcgccgc ctggccgggc gagtacgtcg acggcgtgct
     1141 ctcgcacgcc gaatcgggcc atcccgagtt cgttcgctac acgaatcctg aagccgtgct
     1201 cgtcaacctc ccgcccccgg gctcccacac tccctgatac gcgaggcccc atgcgctatt
     1261 ccgagctgat ccacttcgag ccgatcgaat ccgtcgtcca gctgcggcgg gccgacaaga
     1321 gtgacgaggc gaagaacctc gtccgagcct tcgtgatctc agaccggatg gccgagcagc
     1381 tcacggacct cgtcttcccc gagctgcagt tcgaggtgcc ccacgacaac aagggggtcc
     1441 tggtcgtcgg gaactacggc accggcaagt cccacctgat ggccgtgatc tcggccgtcg
     1501 cggaggacgc cgcgaccgcg aagctcctca cgaacgcgaa ggtggcggac gcggcgaagc
     1561 cgatcgccgg gaagttcaag gtcctccggg ccgagatcaa cacccggatg tcgcttcgcg
     1621 acttcgtctg caacacgctc gacgcgaagc tcggcgagct gggagtcgac taccggttcc
     1681 cctcggagca gaaggtcctc aacccgaagg acgccttcgc cgagatgatg gcggccttcc
     1741 acgagaaata ccccaagcac gggctcctct tcgtcctcga cgagctgctc gactacctcc
     1801 ggtcgagaaa gcagctcgag ctcatccagg acctgaactt cctccgcgcc gtcggggagt
     1861 tctgctcggg gtcccgcttc cggttcgtgg ggggcgtcca ggagagcctc ttcgacagcc
     1921 cgagcttcca gttcgtggcc gagaccgttc ggcgggtcaa ggaccgcttc gaacaggtcc
     1981 gaatcgctcg ggaggacgtc gccttcgtcg tctcccagcg cctcctgaag aaggacgcga
     2041 agcaggaagc ccgaatccgt gagcacctcc tgaagttcgc tcccctctac gggtcgatga
     2101 acgagcggat ggacgacttc gtccgcctct tcccggtcca tccggcctac ctctccacct
     2161 ttgagggcgt ccgcctcgcc gagaagcgcg aggtgttgaa gacgatcagc gcggagatcc
     2221 ggaggctcgt cgaccagacc gtacccgcgg acgccccggg cctcgtcgcc tacgacagct
     2281 actggaagac gctgaaggac aacccctcgt tccgggcggt ggaggacatc aggaaggtcg
     2341 tcgacaaggc ggatgtcctg gaagggcgca tccagcagtc gttcactcgg ccgcaataca
     2401 agcccgtcgc acttcgcatc gtcaacgccc tggccgtcca tcgcctgacg acgggagaca
     2461 tctacgccaa gctcggcgca accgccgagg agcttcgcga cgacctctgc ctcctcatgc
     2521 cgattccgga aagggaagcg gagttcctcc ggtcggtcgt cgagaccgtc ctcaaggaaa
     2581 tcctcaagac cgtcagcggc cagttcctca gcttcaacaa agagaacggc cagtactacc
     2641 tcgacatcca gaaggacgtc gacttcgact cgctcatcga gaagagggcc gagggtctcg
     2701 cccccgaccg gctcgaccac tactacttcg aggcgctcgc ccgcgtcctg gaatgcgtcg
     2761 accagaccta ccggagcggc ttccggatct gggaacacga gctcgagtgg ctcgagcgca
     2821 aggccgggcg cagcggctat ctcttcttcg gcgccccgaa cgaacgaccg aacacgacgc
     2881 ctccgcggga cttctacctc tacttcatcg agccctacga cccggtctac ttcaaggacg
     2941 agaagaaggc cgacgaggtg atcttccggc tcaaggaccg cccggagggg ttcgaggggc
     3001 cgctgaagct gttcgccggg gcccgcgagc aggccgcggc ggcctcgggc gcgaaccgga
     3061 agatctacga cgagaaggcg accgagcatc tcaagctcct gacgtcctgg ctcgctgcga
     3121 acgctccgac ccacctcgag gtaacccatc agggccggac gaagctcctc gccgacgtcg
     3181 tccgggggaa ggcccccgcg agcttcgacc ggtcgtcctt ccgcgatacg gcgaaccttg
     3241 ccgcatcgct tctcctggcc tcgcacttcg ccgaccgcgc ccctgagtac cccaccttca
     3301 cgatgctcgt cacccgtgcg agccgggtcc aggccgccac ggacgccttg aagtggatcg
     3361 gcggaggagg gaagacgaag cagggaacgg ccgtcctcga cgccctggag cttctcgacg
     3421 gggacgtcct tcggccccgt gagtcccgct acggaaagaa cgttctcgag gcgttctccc
     3481 agaagagcgc gaaccaggtt ttgaaccgga acgaactgat ccgggaggac ggtgcgatcg
     3541 agtactggtt cccgttccgg gtggagccgg agctcctggg ggtcgtcctc gccgcgctcg
     3601 ttcacgccgg ggacgttgtc atcagcctcc cgggccgcaa gctcgacgcg agcgggctcg
     3661 acgccctctt gaagatcccg gccgacgaac tggccgcctt caagcatctc gagcgcccga
     3721 aggacctccc gctggcgccg ctccaggagc tcttcgatct cctctccctc ccgcggggcc
     3781 tcatcgtcaa cccggcgaac cgcgaggacg gagtccagaa gctccacgtg gccgccgctc
     3841 agcggatcga gcaggccatt gccgccctcc agagcctcga ctcgggcctc agcttctggg
     3901 gccggcctgt cgtgcccgag aaggagcgga cggcctgggc ctcccagatc cgcgacctga
     3961 agtccttcct cgagtcgctc cagccctaca acacggtcgg caagctgaag aacttcccgc
     4021 acgacgtcgc ggcgatccag gcccgccgca gtgcgttggc aacggtcgag tccgtctcca
     4081 ccctggcggc cctctgccag cagctcagcc cactgacggc ctatctccag acggcggagg
     4141 ccatcctccc ggtggagcac ccctgggtca cgacggtccg ggccgcgaaa gacgagctcg
     4201 tgggtcgcct cgcgacccct aagggccggg tggaagccag cctgcgacgg gacgtcgagg
     4261 tccgcctcgc ggagctgaag gcggaatacc aggccgccta cctcaagctg catggccagg
     4321 cccgcctcgg cgcggaggcc gacaagaaga aggtcgctct cctcagggac ccgcggctgg
     4381 cccagctcca gaaactcgcc agcatcgaga tccttccctc gcatcagctc caggagattc
     4441 agaaccggct cctgggcctc aagacgtgcc tttctgtcgc ccagcaggag ctcgactccg
     4501 ccccccactg cccccactgc ggttttcgcc ccgcggagga ggcgatttcc gccgccccgg
     4561 gcggagtcct tggggaggtc gacgagaggc tcgacaagct cgtccacgac tggacgacga
     4621 ccctcctcga caacctctcc gatccgacga tctcggggaa catcgagctc gtgacggatc
     4681 cgaaggggaa gcaggccctc caggccttcc tccggaagaa gaacctcccc gagcccgtgg
     4741 acagtcacct cgtgaaggct ctccaggagg tcttctccgg tct
//
LOCUS       DAOONL010000203         4710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_21882_length_4710_cov_18.049409, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000203 DAOONL010000000
VERSION     DAOONL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21882_length_4710_cov_18.049409"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLP50_16670"
     CDS             <1..796
                     /locus_tag="PLP50_16670"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPA53227.1"
                     /translation="KSVVALVSKVDFEPIWQEMRARLREELDYLHEAERMRRMAELHA
                     DVPEVVIPRVVEEASTGRILTTEYEEGLSPDEACAPQTSQALKDRWGTVLFAFLLRGL
                     LEHRLLHADPNLANFSFRRDGRVVVYDFGCVKEVPLRLARGYRGLCRAALEGRTDDLP
                     AVLKRMGMHRLDGAPLEEHLVEPWAALVVELLRPAPPYRFGSDDSVARRMVDAARTSL
                     SEAGDVRFPRDIVFVNRTVLGHFGNLARLRAEGPWRDILRRFTESA"
     gene            859..2442
                     /locus_tag="PLP50_16675"
     CDS             859..2442
                     /locus_tag="PLP50_16675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53228.1"
                     /translation="MSNAPGSSYDEVPYVDTCHGATHPDRLATLAAVYGLPAPPVERC
                     RVLELGCARGGNLLPMALELPEASFVGVDLSTRQVAEAREAAEALGLRNVAFHAMGLE
                     EIDAAFGTFDFVVCHGVYSWVPEPVRERIFAICATNLSPDGLAYVSYNTYPGWSSRGM
                     VRDLVRFHVREEETPEGRVERARGFLEELAGHVVRQEGAYASVLKVEASRFREADDTY
                     LLHEFFEDDNAPCFVHEFLRRAAAAGLEFVAEAQAPGLFASLPEAARELVVRWAGDVA
                     AREQYLDLMVNRTFRRSVLRRAGGLRAAAPSPEAVERLAARAVVVPAPVAGEAEGKDG
                     PIEFVHPGRGGSIRTNDPALKAALLALYEVRPAALPFGTLWEHVHESLSRSADSSPRP
                     ATETREALRSSLLRLFLADFLDLHVRPPRPAAEPAERPVGSPLARRQAASGSTVTSLL
                     RRRVELDELARAVLLELDGTRDEEEVLAAVVARVCSGALELSDASGPVREEGVARALL
                     AGEIRPAIGRIARLALLVG"
     gene            2518..3996
                     /locus_tag="PLP50_16680"
     CDS             2518..3996
                     /locus_tag="PLP50_16680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HPA53229.1"
                     /translation="MIAVFVLYMVATVSLAVWYSRGARSHRDFVLGGGRFGGTALALS
                     ERATGESAWLFLGLTGHAYADGLSTIWVALGCVTGILFIWMVMARRLRDETERTGAMT
                     VSSLLARRFPGAERPISLLSAAIVVFFFLLYIAAQFSGSGKVLQETFGLDPLVGILIA
                     SAVVTIYCGIGGFIAVVVTDVLQAVLMIFTLVAFPVIALFAAGGRGLDLAAAFAAAGP
                     DYLSLTGGKTGAAAAILVLSGLSWALGYTGQPQLLTRMMAIRSAGDVKRAVRVATIWT
                     LLAYSGAVLIGMLGVAFAKGGIVGSGGSLADPEKVLPAMVLTLVNPILAGVLLSGVIS
                     AMMSTASSEVTVSSASFSEDIFASLRKKAATARGMLLLNQVATVAVGAIAIVLALTMR
                     DTVYSLVSYAWSGIGSSFGPALVLLLFWKRLSRAGVFASLVTGTVGTIVWKTFLEQPT
                     GVSERLASYLLAFAMAVLFSILLPEKARPVGDAASASPSTAG"
     gene            4220..>4710
                     /locus_tag="PLP50_16685"
     CDS             4220..>4710
                     /locus_tag="PLP50_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPA53230.1"
                     /translation="MTTAANDARIPVAVLGATGAVGQRMVSLLTGHPTLRLAEVAASE
                     RSLGKTYVDATRWILPGDVPGEARDLVVGPVDGPLSSRLVLSALDAAVADDVEPLQAA
                     RGKLVVSNTKSFRMHADVPLLVPEVNAGHLALLDRQEWAAAGGGIVTNPNCVVVGLAM
                     ALAP"
BASE COUNT          565 a         1686 c         1735 g          724 t
ORIGIN      
        1 gaagtcggtc gtcgcgctcg tctcgaaggt cgacttcgag ccgatctggc aggagatgcg
       61 cgcccgcctg cgcgaggagc tcgactactt gcacgaggcg gagcggatgc gccggatggc
      121 ggagctccac gccgacgtgc cggaggtcgt catcccgcgg gtcgtcgagg aggcctccac
      181 cgggcggatc ctgacgaccg agtacgagga gggactctct ccggacgagg cctgcgcgcc
      241 ccagacgtcg caggcgctga aggaccgatg gggaaccgtc ctcttcgcgt tcctcctccg
      301 gggtctcctc gagcaccggt tgctgcacgc cgatccgaac ctcgcgaact tctcgttccg
      361 tcgggacggg cgggtcgtcg tctacgactt cggctgcgtg aaggaggtgc cgctccgcct
      421 cgcccgcggc taccggggcc tctgccgggc cgcgctcgag gggcggacgg acgacctgcc
      481 cgccgtcctg aagcggatgg ggatgcaccg cctcgacggc gccccgctcg aggagcatct
      541 cgtcgagccc tgggccgcgc tcgtcgtgga gctcctccgg ccggcgccgc cgtaccggtt
      601 cggctcggac gactcggtgg cgcgacggat ggtcgacgcg gcgcggacga gcctttcgga
      661 ggcgggagac gtccggttcc cgcgggacat cgtcttcgtc aaccggacgg tcctcgggca
      721 cttcggcaac ctcgcgcggc tgcgcgccga ggggccgtgg cgggatatcc tcaggcggtt
      781 cacggaaagc gcctgagtcc ggaccggacg gggtcgtcgc cgtcgcgggg gcgggctccg
      841 tcccgaggag gtctcccgat gtcgaatgct cccggcagca gctacgacga ggtcccgtac
      901 gtcgacacgt gtcacggcgc gacgcacccc gaccgcctcg ccacgctcgc cgcggtgtac
      961 ggcctcccgg cgcctcccgt cgagcgctgc cgggtcctcg agctcggctg cgcccgcggc
     1021 gggaacctcc tcccgatggc gctggagctg ccggaggcgt cgttcgtcgg ggtcgacctc
     1081 tcgacgcggc aggtcgcgga ggcgcgcgaa gccgcggagg ccctcggcct ccggaacgtc
     1141 gcgttccacg cgatggggct ggaggagatc gacgcggcgt tcgggacgtt cgacttcgtc
     1201 gtctgccacg gggtctactc gtgggtgccc gagccggtcc gcgagcggat cttcgcgatc
     1261 tgcgcgacga acctctcccc ggacggcctc gcctacgtct cctacaacac gtaccccggc
     1321 tggagctcgc gcggcatggt ccgcgacctc gtccgcttcc acgtgcgcga ggaggagacg
     1381 ccggaggggc gcgtcgaacg ggcgcgcggc ttcctggagg agctcgcggg gcacgtcgtg
     1441 aggcaggaag gggcgtacgc gagcgtcctg aaggtcgagg cgtcgcggtt ccgagaggcg
     1501 gacgacacgt acctcctcca cgagttcttc gaggacgaca acgccccctg tttcgtgcac
     1561 gagttcctgc ggcgcgcggc ggccgcgggt ctggagttcg tggccgaggc gcaggcgccc
     1621 ggcctcttcg ccagcctgcc ggaggcggcg cgggagctcg tcgtgaggtg ggccggcgac
     1681 gtcgcggccc gcgagcagta cctcgacctg atggtcaacc ggaccttccg ccggtcggtc
     1741 ctccggcggg cgggcggctt gcgtgccgcc gcgccgtcgc ccgaggcggt ggagcgtctc
     1801 gcggcgcgcg cggtcgtcgt ccccgcgccg gtcgcggggg aggcggaagg gaaagacggg
     1861 ccgatcgagt tcgtccaccc cgggcggggg ggctcgatcc ggacgaacga cccggcgctg
     1921 aaggccgctc tcctcgcgct ctacgaggtg aggccggcgg cgctcccgtt cgggacgctc
     1981 tgggagcatg tgcacgagag cctttcaagg agcgcggatt cctcgccgcg ccccgcgacg
     2041 gagacgcgcg aggcgctccg ctcgtcgctc ctccggctct tcctcgccga cttcctcgat
     2101 ctccacgtcc gtccgccccg gcccgccgcc gagccggccg agcggcccgt cggcagcccg
     2161 ctcgcccgcc gccaggccgc gagcgggagc accgtgacct ccctcctccg gaggcgggtc
     2221 gagctcgacg agctcgcccg cgcggtcctc ctcgagctcg acgggacgcg cgacgaggag
     2281 gaggtgctgg ctgcggtcgt cgcccgggtc tgctcgggcg cgctggagct gagcgacgcg
     2341 tcggggcccg tgcgggagga gggggtggcg cgggcgctcc tcgcgggcga gatccgcccg
     2401 gcgatcgggc ggatcgcgcg cctggcgctc ctcgtcggct gagccggccg cccggccggg
     2461 cgccggggcg gcgattgcgc ccttgcccgg cgaccccggc cttcctatgc tcgaaagatg
     2521 atcgccgtct tcgtcctcta catggtcgcg acggtttcgc tcgcggtctg gtattcgcgc
     2581 ggggcgcgct ctcaccgtga cttcgtcctc ggcggcgggc gcttcggagg gacggccctc
     2641 gcgctctcgg agcgggcgac gggggagtcg gcctggctgt tcctgggcct caccggtcac
     2701 gcgtacgccg acgggctgtc gacgatctgg gtcgccctcg gctgcgtcac cgggatcctc
     2761 ttcatctgga tggtcatggc gcgacggctg cgagacgaga cggagcggac gggggcgatg
     2821 accgtgtcga gcctcctggc acgccgcttc ccgggggccg agaggccgat ctcgctcctc
     2881 tcggcggcga tcgtcgtctt cttcttcctg ctctacatcg ccgcgcagtt cagcggctcc
     2941 gggaaggtcc tccaggagac gttcgggctc gacccgctcg tggggatcct catcgcttcg
     3001 gccgtcgtga cgatctactg cgggatcggc ggcttcatcg ccgtcgtcgt gaccgacgtc
     3061 ctccaggcgg tcctgatgat cttcacgctc gtcgcgttcc cggtgatcgc cctcttcgcg
     3121 gcgggcggac gggggctcga cctggcggcc gcgttcgccg cggcgggacc cgactacctc
     3181 tcgctcaccg ggggaaagac gggcgccgcc gccgcgatcc tcgtcctctc gggcctcagc
     3241 tgggcgctcg gctacaccgg ccagcctcag ctcctgacgc ggatgatggc gatccggagc
     3301 gcgggcgacg tgaagagggc ggtgcgcgtg gcgacgatct ggacgcttct cgcctattcc
     3361 ggcgcggtcc tgatcgggat gctcggcgtc gccttcgcga agggcgggat cgtcggctcg
     3421 ggcggctcgc tcgccgaccc ggagaaggtc ctgcccgcga tggtcctcac cctggtgaat
     3481 ccgatcctcg cgggagtcct cctctcggga gtcatctccg cgatgatgtc gaccgcctcg
     3541 tcggaggtga ccgtctcgtc cgcctcgttc agcgaggaca tcttcgcgag cctccggaag
     3601 aaggccgcga cggcgcgcgg gatgctcctc ctgaaccagg tggccaccgt cgccgtcggc
     3661 gcaatcgcca tcgtcctcgc cctgaccatg agggacaccg tctacagcct cgtctcctac
     3721 gcctggtcgg ggatcggatc gtcgttcggg ccggcgctcg tcctcctcct cttctggaag
     3781 cggctgtcgc gggccggggt cttcgcgtcg ctcgtgaccg ggaccgtcgg gacgatcgtc
     3841 tggaagacgt tcctcgagca gcccaccggc gtctccgagc gcctcgcgag ctacctcctc
     3901 gcgttcgcga tggcggtcct cttctcgatc ctcctccccg agaaggcgcg ccccgtcggg
     3961 gacgccgcga gcgcctcgcc gtcgacggcc ggttgacacg ccccgccgtc cacacccaga
     4021 atccgccgcc aatgagcccc gtcaccggcc gaaagcggac gacgaagacg acgatgcgcg
     4081 cgccgcgagg cgggccggcg ggggaccgta cgagcgtgta ggacgcggtc gaacggaacc
     4141 gatagagaag ccagagcccg ccggatcggc gggctctttc atttccggcg cacctgccgg
     4201 aggagagcga agggacgaca tgacgacggc agcgaacgac gcgcggatcc cggtggcggt
     4261 cctcggagcg acgggggccg tggggcagcg gatggtgtcg ctcctcaccg ggcacccgac
     4321 gctccggctc gcggaggtgg cggcctcgga gcgctcgctg gggaagacgt acgtcgacgc
     4381 gacgcgctgg atcctcccgg gcgacgtccc cggggaggcg cgggacctcg tcgtgggacc
     4441 ggtcgacggg ccgctctcgt cgcggctcgt cctctcggcc ctcgacgcgg cggtggccga
     4501 cgatgtcgag ccgctgcagg ccgcgcgcgg gaagctcgtc gtctcgaaca cgaagtcgtt
     4561 ccggatgcac gcggacgtcc cgctcctggt cccggaggtg aacgccggcc atctcgccct
     4621 cctcgaccga caggagtggg ccgcggcggg gggcgggatc gtcacgaacc cgaactgcgt
     4681 cgtcgtgggc ctcgcgatgg ccctcgcgcc
//
LOCUS       DAOONL010000204         4681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22052_length_4681_cov_11.120623, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000204 DAOONL010000000
VERSION     DAOONL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22052_length_4681_cov_11.120623"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLP50_16690"
     CDS             complement(<1..715)
                     /locus_tag="PLP50_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53231.1"
                     /translation="MIRRARSLASLLCALVVLGAVSCGGKAPMPHMDDEETNADIVGP
                     TDRQLELTLDAEVLIDATTSMSGYATVEGSVFNRFVEELEAAIRSGAKQAKLAFFKFG
                     TAVRPIEREVAVLRKPAAFFAEKGIFEQTRLEDAIDSPDPAKDRIILTDLFQNEGDVN
                     KVVAAFKEKCFDRGRDVGILMVRSEFDGVVYDAKAPPYRYVSKAVEPATNRAFFAIFV
                     GDAARFGRIVEALKSTRVAS"
     gene            complement(1445..1885)
                     /locus_tag="PLP50_16695"
     CDS             complement(1445..1885)
                     /locus_tag="PLP50_16695"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HPA53232.1"
                     /translation="MGLFDSFRAAAAPSFNVQQAIMTIVVGAVKADGQVSREEVIRLR
                     SICALSPVFMANTGEQDGAVISFADNVTTQLGDEAIARAIEVLTPELRETAFAFACDM
                     ILADGVLGRDEEVYITKLASKCAIPDETGKAIIYTTIVRNRSAS"
     gene            complement(2151..2396)
                     /locus_tag="PLP50_16700"
     CDS             complement(2151..2396)
                     /locus_tag="PLP50_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HPA53233.1"
                     /translation="MVQVPPNLAIAAKSMWGAAPSTSEIAAQYLERVVNDMAARGYAF
                     ERVDEIGVRSTPGCLAGFLGAKETMVVYYVITFSINA"
     gene            complement(3101..3286)
                     /locus_tag="PLP50_16705"
     CDS             complement(3101..3286)
                     /locus_tag="PLP50_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53234.1"
                     /translation="MAGDAAASPRSGSVGPEVRQAEEPLSELQVTDRGPFEVQGNSDR
                     ENASKLIAEDARITYPK"
     gene            complement(4144..4500)
                     /locus_tag="PLP50_16710"
     CDS             complement(4144..4500)
                     /locus_tag="PLP50_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53235.1"
                     /translation="MVLIAGLEDDLENAEIQAWGRRDTGWEWRLLNGGPDVIDVIAAL
                     RPDVLTTGNRHSLVGGVDLLRLVRDRGATRVAPVVLVTAADKEDLWSAIRVIGLDPDA
                     HIVGYVQRPFLMSRLQ"
BASE COUNT          813 a         1653 c         1331 g          884 t
ORIGIN      
        1 ccgacgctac gcgagtcgat ttcagcgcct cgacgatgcg cccgaacctc gcggcatcgc
       61 cgacgaagat cgcgaagaag gcacgattcg ttgccggctc gaccgccttc gacacgtaac
      121 ggtagggggg tgcctttgcg tcgtagacga cgccgtcgaa ttcggaacga accatcagaa
      181 ttccgacgtc acggccccgg tcgaagcact tctccttgaa cgcggcgacg accttgttca
      241 cgtcgccttc gttctgaaag agatccgtta ggatgatcct gtctttcgcc ggatcgggtg
      301 aatcgatcgc atcttccagc cttgtctgct cgaagatccc cttctcggcg aagaacgcag
      361 ccggcttgcg cagaacggcc acttcccgct cgatggggcg gacggcggtg ccgaacttga
      421 agaaggcgag cttagcctgc ttcgcgcccg atcgaatggc cgcctcgagc tcttcgacga
      481 accgattgaa gacgctcccc tcgacggtcg catagcccga catcgatgtc gtcgcgtcta
      541 tgaggacctc cgcgtccagg gtgagctcga gttgcctgtc ggtcggcccc acgatgtcgg
      601 cgttcgtctc ctcgtcgtcc atgtgcggca tcggggcctt gccgccgcac gagacggcgc
      661 cgaggacgac gagcgcgcag agaagcgacg cgagagaccg ggctcgccgg atcaccgccc
      721 gcctccaccg gtccgctctt cgcctcgccg gcgccggccc tggcccgacc actggtcggc
      781 ggcggcgttc cgccgtgcgt cctcatctcg gcccctcgtc cctagacgat tccactcttc
      841 tgatgtcacc gccattcccg acctcccctt agcggcttcc cgctccctct cgtcaaaccg
      901 ccatcggcta tccgggtggc gggtgcggct ctcggctctg cttgtgcact tctcatgccg
      961 tgacgatcgt gaacgtctct tatcgccatg tccgcgaata gcgcagggcg cgaccatctc
     1021 ccgcccccgt ggagagcagt ccgctcgctg ttagcgcgcc tccactccgg gcttcggcag
     1081 caagacgcca gcgaacgcga ccaccatgcg cagtcgcgcc aaaggcgtct acgacccctc
     1141 ggagcgccat tttgcgaatg cgtctcccgc ttcaatccct gatcagcgac cgaactcgac
     1201 cgaccggccc cggccggcag gccccgcgga cgcgccaccc gctcgccgga acaggtccac
     1261 cctgacaccc gaaaatggct gcccaaggtt ggtccgccga cccgtccacc cgcaactcgc
     1321 cgaaaccttg agtcagagcg atcgcctcgg acctagtccc tccgctcacg agcgccctcg
     1381 acgcgcacga cgatcgtccg tcggtcatcg cccggcactg ccatcaacgc ccgcccgggc
     1441 tgcgctacga agcggaacga ttccgcacga tggtcgtgta gatgatcgcc ttccccgtct
     1501 cgtcgggaat cgcacacttg ctcgcaagtt tcgtgatgta gacctcttca tccctcccca
     1561 ggacgccgtc cgccaatatc atgtcgcaag cgaaggcgaa ggccgtttcc cgaagttcgg
     1621 gagtcagtac ctcgatcgcc ctcgcgatgg cctcgtcgcc cagttgcgta gtgacgttgt
     1681 ccgcgaagga gataacggca ccgtcttgtt ctccggtgtt cgccatgaag accgggctaa
     1741 gcgcacagat cgaacgaagg cgaatgacct cctcccggga cacctggccg tcggccttta
     1801 cagcgccaac gacaatggtc atgattgcct gctggacgtt gaagctcggg gctgccgccg
     1861 cacgaaatga atcgaacagg cccataacac ctccatatcg ttgatggtcg cgactctgcc
     1921 cagcataccc gcgcgttcct ctccggctcc cgatcggcgc cgggaggaac tctcgccgtc
     1981 gcggctctcc tgcgacgcag tcagcgagtc gtattttccg aggagcggcc gttccgcatt
     2041 tctgactacc tcctccgaac gcatccgcag gtccgctcct cgatgccgct tcgttgcgga
     2101 gaaggcaaat catgtcgcag gttgcggaag tagaggaacg accgggatag tcacgcgttg
     2161 atcgagaagg taatgacgta gtagacgacc attgtctcct tcgctccgag gaatccagcc
     2221 aagcagcctg gcgtcgatcg gacgccgatt tcatccaccc tctcaaaggc atatccccgt
     2281 gcggccatgt cgttgacgac gcgctcgagg tactgggcgg cgatctcgct ggtcgaaggg
     2341 gccgcgcccc acatcgactt cgccgcgata gcgaggttcg gcgggacctg aaccatcttg
     2401 taggtcttca gattatcctc ctggcctgag ttcactcgtc actgtcacca tcggacgaac
     2461 gttcctccat ctgcctcacg cgtcaccgcg gcgcgggcta aggtcacggc ccctttcggc
     2521 ccacgcgcgt gcttcgctga gcacccgcgt ctggccgtac ggccgccgca tactcactcg
     2581 tcagcgccgt aacacctccc agctctttcc ctctttcgtc gcaccacaat tcgggcagta
     2641 gaggccgcct agcgggccgc tcgctttcgt ccagcactcg ctgcaactcg cttcaccgca
     2701 ttcgtcgcac ctgtacttcg gcgattcatc ccgaccacac ttgatgcact tcaactggca
     2761 cctcgctcat tcgccatcct gacgttttct tcgagccaga ccgagggaac actcacgcaa
     2821 cggcagggat accgtgctgc agcaatcgcg cgaccgggat tcactccatc ttgctgtatt
     2881 cggaagacag gaacttccgg gcttcgcgaa ttctcttgta tctgttctcg acgccccact
     2941 tgtagagtgt cgacctccgc cctgattgtg tcatccaagg ctctacacct gtcagcacgc
     3001 gcgtgtcgct ctatagcgcg gcactcactc cctcgcactc ggtgtacgga aatgaactat
     3061 tcctcttgag gggcgagagg aactcggcct tggagtagct ctacttcggg tacgtgatcc
     3121 tcgcgtcctc ggcgatgagc ttcgaggcat tctccctgtc gctgtttccc tgcacctcga
     3181 agggccctct gtcggtgact tgaagctcgc tcagaggctc ttccgcttgc ctcacttcgg
     3241 gcccgaccga gcccgatctc ggcgaggccg ccgcgtcgcc ggccacggcg ccgcactcgt
     3301 gggcgtctcc gtcgaccgag acgagtcgag gcgttctgct cgtgaatcac tgtgacgctc
     3361 ccgcagagta ggccaacgtc ggcatccagc tcgccggcct gcatcctcag attcaacttg
     3421 agcttcctgg tgtcacagga gaagtacgtg cccatgtcgg agcgcagcgt gccccaagac
     3481 ttgcggctac caacctcttc gcgtttggtg agactctgca cgaccaaggt atccgcttcg
     3541 gcgaggggca ctgcgacgag aaggcggtct tcgacggtgc acacagcgac gagactcggc
     3601 gaggccagcg cagccgcgag atttcgtgtt gcgagaatcg actcaaggcc gccggggatt
     3661 ctctgaaagc ggttcgcacc gcttctcact gctacggcct cctcggcggt ggaggaagac
     3721 gccatgtgtc ccggatcggg aatcgttgcc ccaagtatca cccgatgatc gatcagagct
     3781 cccacgaccc tcatcaccac ctcgccgaat gcaacacacc tgggcgcccc cgcgactgcc
     3841 ccttctcgcc aacctaatct gcaagtatcg cgccgcgaac ggggacgacc tcaagctcaa
     3901 catcgacgag cgtgtgcgcg agacgtgaag atcgtcgcgc aagaagcgag cagaggtgcg
     3961 cgaatcaggg gcgaggcgat cgatgcaaga tgtccagcgc agcgatccga cgaacgagca
     4021 gcagtagccg cgtcgctggg cgctccggtt ggacctctta cgttcttgca gcgcaacgta
     4081 tgtccgcgtc aggtccctgg cgccctggtg cggccgccgc cccctcagta tccggcggcg
     4141 ttcctactgc agcctgctca tcaggaacgg cctctgaacg tacccgacga tgtgggcgtc
     4201 cgggtcgagc ccgatgaccc ggatggctga ccagaggtcc tccttgtcag ccgccgtaac
     4261 aagaacgaca ggggctaccc tcgtcgcgcc gcgatccctc acgaggcgga gcagatcgac
     4321 tccgccaacg agggagtgac ggttccccgt cgtcaggacg tccggccgga gcgccgcgat
     4381 cacgtcgatg acgtctgggc cgccgttaag gagtcgccac tcccacccgg tatctcggcg
     4441 gccccaggcc tggatctccg cgttctccaa gtcgtcctcg agccccgcga tcagcaccat
     4501 cggccacccc ctttcacaac gtccggcatc cggtgcgccc taccctacct cgccctcaac
     4561 cccaccacgc aggtcaggag gcaggcgaag tcgccgatct cccctcgctc gagtccgatc
     4621 gcccgccgcc aggagttgtc ctcgccgatc gcggccatcg cgcggctcag gatattgcag
     4681 g
//
LOCUS       DAOONL010000205         4652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22233_length_4652_cov_19.567544, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000205 DAOONL010000000
VERSION     DAOONL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22233_length_4652_cov_19.567544"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLP50_16715"
     CDS             <1..621
                     /locus_tag="PLP50_16715"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPA53236.1"
                     /translation="PSQQMMMGIDEKPFLNDEEKIVPIERFGVRVMSLGFLMDVDTPV
                     IWRGPMIYKAIEQFLGDVAWGKQDFLIVDLPPGTGDAQLTLTQKVALSGAVIVSTPQD
                     VALIDARKGLAMFQKVNVPVLGLIENMSGFECPKCGHVEPIFKTGGGERTARELNVPF
                     LGRVPLDPRVALSGDAGMPIVAAEPDSASTKAFLDLGQAVAKAVGA"
     gene            1156..2484
                     /locus_tag="PLP50_16720"
     CDS             1156..2484
                     /locus_tag="PLP50_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53237.1"
                     /translation="MTRTDRRIGRALALLAFLALAALGHHTWGTVDAIQRLAVTNAVL
                     TRGSVVTPEYGPVKYGPLQPFLMLPTYALGYGIGELARAPEPGRVGYRVTAFLFTPVL
                     VSLLVYLWYGFARRWGQDRASAAVGAWTLLWCTLVLPYSRLLFSELLSATMLLAGVAY
                     LCVPRDEDRRPVGFFFLALASLNYVVFVPVLLVACAALPLYELGRARPGEARRAIISG
                     AAAILFALVGWGAYNVARYGTPLRFGYAGESFSTPLLEGLYGLLASPGRGLLYYSLPT
                     AAAVVLAVRGVATCRDPAGVRDASALLLFGSYLLLYARWGSFEGGWCWGPRFLIPFIP
                     LVHVALLALLPRLRVAFYSVCAAGLLANAWEYSIEWGPYEKAMFGSGVIDYRRSVFEL
                     EYVSALHGFAGSTSIERLLQFAGIAFLSSFLVARVVRRHIAAGESGEATG"
     gene            complement(3088..>4652)
                     /locus_tag="PLP50_16725"
     CDS             complement(3088..>4652)
                     /locus_tag="PLP50_16725"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HPA53238.1"
                     /translation="QKTREKHRKINVILAILAFCCILYGTFLTRSGVLADFSVHSFVD
                     LGITGWLVAIILVFLFGGLALLAYRWREIPVVNEIDPATGLEKEEPFLSRSVLFILAV
                     TLFCASGLVILLGTSAPILTRIGGKASQVATSFYDVTHTPLAIVMALLIALVPFVSWR
                     GETWAAVGKKVALPALLGALGVVLFLFLGVRKPVDLLFLGAAVFGLASSAEILVLFVR
                     RKAIASAGGYLAHVGTAIMLVGILISGAYEVKTQVTLHRNRPLVIEQHGRPISFTFTG
                     LYFVNDDGVVKTQSQLDMTKLADRRAKQAMEVTVSDGKATWKAYPKLYTNEKSGQLMA
                     NPDVRKSFFFDLYVSPQAYEPGAPPRAEGALAVLRKGEEKTVHGVNLRFLEFSTEGAH
                     TEGGAFTIGTKLAVNSAGATSEVTPKFVVTLGGSEGQRFENPLSPLPGTGAGQVRIRR
                     VSPNDAAVELELLGLDPAGDLKPAVPESFSIDVTTKPLIALVWAGFYVMMAGGLVSMI
                     KRAKDARQAAIA"
BASE COUNT          643 a         1563 c         1691 g          755 t
ORIGIN      
        1 ccgtcgcagc agatgatgat ggggatcgac gagaagccgt tcctcaacga cgaggagaag
       61 atcgtcccga tcgagcgttt cggcgtccgc gtcatgtcgc tcggcttcct gatggacgtc
      121 gacacgccgg tcatctggcg cgggccgatg atctacaagg cgatcgagca gttcctcggc
      181 gacgtggcgt gggggaagca ggacttcctc atcgtcgacc tcccgcccgg tacgggcgac
      241 gcgcagctga cgctgacgca gaaggtcgcc ctctcggggg ccgtcatcgt ctcgaccccg
      301 caggacgtcg ccctcatcga cgcccgcaag gggctcgcga tgttccagaa ggtgaacgtc
      361 cccgtcctcg gcctgatcga gaacatgagc ggcttcgagt gcccgaagtg cggccacgtc
      421 gagccgatct tcaagacggg cggcggcgag cggacggccc gggagctgaa cgtcccgttc
      481 ctcggccgtg tcccgctcga cccgcgcgtc gccctctccg gcgacgccgg catgccgatc
      541 gtcgccgccg agcccgactc cgcctcgacg aaggccttcc tcgacctcgg ccaggccgtc
      601 gcgaaggccg tcggggcgta accagacccc tggggggtac cggggcacga gagcacgggg
      661 gaggggagcc tcaccccggg ggtgggtccc acggccgctc gccggggggc ctgagcttcg
      721 cgcggccccc cggacccccc cactctctct tcaagcggcg ctgccgttcc ggtggctccc
      781 tggctccgcg cggccgtccc gcttacgaat ccgctgcggt cgggcggggc gcccgcgcga
      841 gctcctcgct gcgctcgtca gacacgcgcg cgggcttcac ccccacccgc tcctcgcgta
      901 ttcgagcggg tccccggccg tgctcggggg cggctcgcgg gacccacccc cgtggctcgg
      961 ccgcccggag cgatcacgag taggggtcat cacgagtagg ggtcagatct accttctaca
     1021 ctctacgggt ggcgtgcggg ctcgcggcgc ggcggtcggc gtcgtcggaa tcgacgacgc
     1081 cgatcggaag ggccggagtg ccgtcctggg cctggctggt tgatgtgcct ctcggacgcg
     1141 acaatgtggg gactgatgac cagaaccgat cgacgcatcg ggcgggcact ggccctgctg
     1201 gccttcctcg ccctcgccgc gctgggccac cacacgtggg gcacggtcga cgcgatccag
     1261 cgactcgcgg tcacgaacgc cgtgctgacg aggggaagcg tggtcacgcc cgagtacggg
     1321 ccggtgaagt acgggcctct tcagccgttc ctcatgctgc ccacgtacgc gctcggctac
     1381 ggcatcggtg agctggctcg ggcaccggag cccggtcgcg tgggctaccg agtcaccgcc
     1441 ttcctgttca ctcccgtgct cgtcagcctg ctggtttacc tctggtacgg gttcgcgaga
     1501 cgctggggcc aggaccgggc gagcgcggct gtcggagcgt ggacgcttct ctggtgcacc
     1561 ctggtgctgc cgtattcacg cctcctcttc tcggagctgc tgtcagccac catgcttctc
     1621 gcgggcgtcg catacctgtg cgtcccgcga gacgaagacc ggcgtcctgt cggattcttc
     1681 ttcctcgcgc tggcatcgct gaactacgtg gtcttcgtcc cggttctcct cgtcgcgtgc
     1741 gcggcgctac cgctctacga actggggcga gctcggcccg gtgaagcgcg cagggcgatc
     1801 atctcgggag cggccgcgat cctgttcgcg ctcgtcggct ggggtgccta caacgtcgcg
     1861 cgctacggta cgccgctgcg cttcgggtac gccggcgagt cgttctctac ccctctcctc
     1921 gaagggctct acggtctgct cgcgtcgccg gggcgcgggc tcctgtacta ctcgctcccc
     1981 acggccgcgg ccgtcgtcct ggccgtccgt ggtgtcgcga cctgtcgcga tccggcaggg
     2041 gtgcgagacg cgtccgcgct gctcctcttc ggctcgtacc tcctgctcta cgcgcgctgg
     2101 ggatccttcg aagggggatg gtgctggggg cctcgctttc tgatcccgtt catccccttg
     2161 gtgcacgtcg ccctgctcgc gctccttccg cgactccggg tcgccttcta ctcggtctgc
     2221 gccgcgggcc tcctggcgaa tgcgtgggag tactccatcg agtggggtcc ctatgagaag
     2281 gcgatgttcg ggagcggcgt gatcgactat cgccggagcg tcttcgagct ggagtacgtc
     2341 tccgcgctgc acggcttcgc gggctcgacc tcgatcgagc gccttctcca gttcgcggga
     2401 atcgcgttcc tgagctcctt cctcgtcgcg cgagtcgttc gccgccacat cgctgcggga
     2461 gagtccggcg aggcaaccgg ttgaggacac gcgagtcagt tccgacccac tcgaattcag
     2521 gtctgccatc tacgctctac gcaccgactg cgagtgggga ggaacgcggt gtcgtcgtgc
     2581 cgaaggtcga ttccgcgatc gcaccgcgcc gatgtgctcg agtcttgccg cgtcgggggg
     2641 ccctcgtgcc gcccgcaggg cggccgcaag cgttgacatt gcctggcgcg ggggaggggt
     2701 gcgccccgcg aggcgaccgt tcgagcacgc gcggggcccc gcacggctcc gcgagcaggc
     2761 cgccggggga agagcgcgca ccgattcgag gagcgcagcg acgagcttgc gcgctcgccg
     2821 gtggcccgcg cagcggggcc gaggacgcag aagcatgcga gagctcctgt gtggttgggg
     2881 gtccgggggg cgcgcgaagc tcagcccccc ggcgagcgcg ggtcgcgcgc gcgcagaggg
     2941 cgccggcggg gcgcacccct cccccgcgcc cccggatcgt ggacgagcgc ttcgcctcca
     3001 ccggaaacgg cccggcgttt ctgccgggcc gttatcaact cgaagagctc tgggtgctcc
     3061 gggtcgctcc ccggtccccg ccccggctca cgcgatcgcg gcctggcggg cgtccttcgc
     3121 gcgcttgatc atggagacga ggcctccggc catcatcacg tagaagccgg cccagacgag
     3181 ggcgatgagg ggcttcgtcg tgacgtcgat ggagaaggac tcggggacgg cgggcttcag
     3241 gtcgccggcc gggtcgaggc cgaggagctc gagctcgacg gcggcgtcgt tcggggagac
     3301 gcggcggatg cggacctggc cggcgccggt gccggggagg ggcgagaggg ggttctcgaa
     3361 gcgctgtccc tcgcttccgc cgagcgtgac gacgaacttc ggggtgacct ccgaggtcgc
     3421 gcccgcggag ttgacggcga gcttcgtccc gatcgtgaag gcgccgccct cggtgtgggc
     3481 cccttccgtc gagaactcga ggaagcggag gttcacgccg tggaccgtct tctcctcgcc
     3541 cttccgaagc accgcgagcg ctccctccgc gcggggcggg gcgccgggct cgtaggcctg
     3601 cggcgagacg tagaggtcga agaagaacga cttcctcacg tcggggttcg ccatgagctg
     3661 gcccgacttc tcgttcgtgt agagcttcgg gtaggccttc caggtcgcct tgccgtcgct
     3721 gacggtcacc tccatcgcct gcttggcgcg gcggtcggcg agcttcgtca tgtcgagctg
     3781 ggactgcgtc ttcacgacgc cgtcgtcgtt gacgaagtag aggccggtga acgtgaagct
     3841 gatcgggcgg ccgtgctgct cgatcacgag cgggcggttc cggtggagcg tgacctgggt
     3901 cttcacctcg taggcgcccg agatcaggat gccgacgagc atgatcgccg tgccgacgtg
     3961 ggcgaggtag cccccggcgc tcgcgatcgc cttgcgcctc acgaagagga cgaggatctc
     4021 ggcggaggag gcgaggccga agacggccgc gccgaggaag aggaggtcga ccggcttcct
     4081 cacgccgagg aagaggaaga ggacgacgcc gagggcaccg aggagcgccg ggagggcgac
     4141 cttcttcccg acggccgccc acgtctcgcc gcgccaggag acgaacggca cgagggcgat
     4201 gaggagcgcc atgacgatgg cgagcggcgt gtgggtgacg tcgtagaagc tcgtcgccac
     4261 ctgcgaggcc ttgccgccga tgcgcgtgag gatcggcgcg gacgtgccga ggaggatgac
     4321 gaggcccgag gcgcagaaga gcgtcaccgc gaggatgaag aggacggagc gcgagaggaa
     4381 cggctcctcc ttctccaggc cggtggcggg gtcgatctcg ttgacgaccg ggatctccct
     4441 ccagcgatag gcgaggagcg cgaggccgcc gaagaggaag acgaggatga tggcgacgag
     4501 ccagccggtg atcccgaggt cgacgaagga gtggacggag aagtcggcga ggacgccgga
     4561 gcgggtcagg aacgtgccgt agaggatgca gcagaacgcg aggatcgcga ggatgacgtt
     4621 gatcttccgg tgcttctcgc gcgtcttctg ca
//
LOCUS       DAOONL010000206         4637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22327_length_4637_cov_13.920995, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000206 DAOONL010000000
VERSION     DAOONL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22327_length_4637_cov_13.920995"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..465)
                     /locus_tag="PLP50_16730"
     CDS             complement(127..465)
                     /locus_tag="PLP50_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53239.1"
                     /translation="MFRITEEPGTDEVRVRLEGDLTGTGVQVLEDFWSTTGSSYASGR
                     VLFDLSGVCRVDDAGRFLLALIHQTGVRMCASGVEMRALVRSIDQDWPMCHLDGPGWR
                     AVGSPVKPHR"
     gene            1076..2581
                     /locus_tag="PLP50_16735"
     CDS             1076..2581
                     /locus_tag="PLP50_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53240.1"
                     /translation="MEPSPGSFDLTALRSRITEVTQHGKPYSLAIGAGGPGTPAWLLD
                     SLGAPYVDYSFRGTPMRLALSWSPVVRERLRVLAARLGQELGSDPALKLVYVTQMTSN
                     GIEGHLLGVDMTAFSRAGYTDDAWVEAALDTAAAFADAFPAKALAFEVHDVNGGSSVP
                     ARILEALRTDSCFGGRVGAAIWWVSGRTDYQPALLDVLRTFPGDKYGQVIARSDDSTR
                     FASGDYATVFAQAKELGLRYLEPWEYDFGAGAGTANGKWDALLRDFNAWTAGLAVPQA
                     TWIVPSSARAPGAGGSFYTTDLVLANPGGDAIVTLRFLGHDRDGRSGETASFSLPAGT
                     SRTTGDVIGSAFGSVEAWGAIQVVSDEPSLVALACTSTRFGNGTFGQCVPAFGDEGRL
                     GAGRSWTIAGVREDGHFRTNLILANGAETETGVDMTLLADDGRVLGSTTFTLPPLGMT
                     QVSRVVRALGVVGEVSGARIVLSTRSSGASLAAYAALIDETTNDPRTLLPR"
     gene            complement(2803..4245)
                     /locus_tag="PLP50_16740"
     CDS             complement(2803..4245)
                     /locus_tag="PLP50_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPA53241.1"
                     /translation="MNATAKVGLFAMAVLLVLAALVLKIEDLPLPGKARRATTEVVFK
                     DVAGLDDKSAVRIAGVRVGRIDGIRLMPDGTAVARLLFDADVELRDGAYGQVKNLGLL
                     GDKYVDLYAGDPAKPRLPDGTRIPGSVPVEFDQLTKLAQEIGRDVKAISGALSSSLGG
                     ETGEAKINRIVDNVGALTEELRLLVAANRANVDVTVANLKEFSASIRETLARLDRILD
                     ENRTSVKGSVVNIEELSEKLKTSADNLNSITGKIDKGEGTIGKLVNDETSHRNLNEAF
                     TSIKTGVEELRTTLTRINRIDLDLGFYGEYATRTEEWRGAFRIDVTPREGKFYRIELL
                     GLPNGRRVDETYRYETSVDGGPPSVVTTKVTRTEDKFGLSAQLGRRYKDTTLRAGLIE
                     SRGGFAIDQHLLKDRLQLSAEAWDFGRADGDAQVKLYGQWQSRGAVFLRAGVTDVINP
                     ERRSFFLGAGIRWKDEDIKTLLPSALGALR"
     gene            complement(4242..4622)
                     /locus_tag="PLP50_16745"
     CDS             complement(4242..4622)
                     /locus_tag="PLP50_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA53242.1"
                     /translation="MEERRPAELSGGMRRRVGFARAIALKPEILLLDEPTTGLDPITT
                     DVLTRVIVRMRDTLMPTMVTITHDLKVAFAVAERVALLDGGRIVADLPPREFAASSDP
                     RVAAFVRGDASEELEAAGSEKGSE"
BASE COUNT          689 a         1604 c         1530 g          814 t
ORIGIN      
        1 cggtccatcg aggtggcaca tgggccagtc ctggtcgatc gatctgacga gtgccgcctt
       61 ctcgacccgt gctcgattcc ggcggtgtgg ggccgcatcg cgtcgtgccc tcgacggcga
      121 ggaaggtcat cgatgcggct tcactggcga tcccacggct cgccagcccg gtccatcgag
      181 gtggcacatg ggccagtcct ggtcgatcga tctgacgagt gccctcatct cgaccccgga
      241 tgcgcacatt cgaacaccgg tctggtgaat cagggcgagt aggaagcgcc cagcgtcgtc
      301 cacgcggcag accccggaaa ggtcgaacag gacgcgaccg ctggcgtagg acgaacccgt
      361 cgtcgaccag aagtcctcca gaacctggac tcccgttccg gtgagatcac cttcgagtct
      421 cactcgaact tcgtcggtgc cgggttcctc ggtgattctg aacatcgccg ttgcctcgcg
      481 atcggagatc gcaagggaga tgcccgagac cgcagggcta ctgtaagcgt gcgtaaacat
      541 ctgtgcagga aagtgataag caagtgctgc gcgtcgtggc aaaggagccg gtgccggccg
      601 tgcggtgccg agtattcggc agccggagtc tgcgcgacgg aaaccggcaa cggatgctga
      661 tcatcgcgtg gccgtaacag acagcgctgg tggcggtgag gcgaaggagg gtgccaagcc
      721 cgtccgctgt gccagctatt cggcagcaag ctgccgcact ggacagacga tgaaggcgtt
      781 ctgcgctgcc tcccctccgg attccccgcg tctcgccctt tccgggtccc gatttccgcg
      841 taaaccggcc gtgcccggca tgcgtccacg gttcatggcc tcttcgcttc cggcgcctcc
      901 cgtcctgagc ccggctgtgc ggcttctgct cgtcgcgtcg ctcgcgcttt ctgcgaccca
      961 tatccgcgct gcgccggtcg agaagccggc cggcgccttc gcgagcgcct ctggcccgac
     1021 gatttgggct caccccgagc tgcacggcgt tctcgtccgg gcgagttgga gcagcgtgga
     1081 gccgtcgccc gggagcttcg acctcacggc gctccgctcg cgcatcacgg aggtgacgca
     1141 gcacgggaag ccctactccc tcgccatcgg tgccggcggc cccgggacac cagcgtggct
     1201 cctcgactcc ctcggagccc cgtacgtcga ctactcattc cgcggtactc cgatgcgcct
     1261 cgctctctcc tggagcccgg tcgtgcggga gcggctccgc gtgctcgccg cgcggctcgg
     1321 tcaggagctc ggaagcgacc cggcgctgaa gctcgtctac gtcacgcaaa tgacgtcgaa
     1381 cggaatcgag gggcacctcc tgggtgtcga catgacggcc ttctcgaggg cgggctacac
     1441 ggacgacgcc tgggtcgagg ccgcgctcga cactgccgcc gccttcgcgg acgccttccc
     1501 cgcgaaggcg ctcgcgttcg aggtccacga cgtgaatggc ggctcgtcgg tccccgcgcg
     1561 gatactggag gcgctccgga ccgactcctg tttcggaggg agggtaggcg ccgcgatctg
     1621 gtgggtttcc ggaagaaccg actaccagcc cgcgctgctc gacgtcctcc ggaccttccc
     1681 gggcgacaag tacggtcagg tcatagcccg ctccgacgac tcgacgcgat ttgccagcgg
     1741 cgactacgcg accgtcttcg cgcaagcgaa ggagctcggc ctccgctacc tcgagccgtg
     1801 ggagtacgac ttcggcgcgg gagcggggac cgcgaacggg aaatgggacg cccttctccg
     1861 ggacttcaac gcctggacgg cgggcctcgc cgttcctcag gcgacctgga tcgtcccctc
     1921 gtcggcgcgc gcccccggcg cgggaggcag cttctacacg acggacctcg tcctcgccaa
     1981 cccaggcggg gacgccatcg tgacccttcg cttccttggg cacgaccggg atgggcgaag
     2041 tggcgagacg gcctcgttct ccctcccggc cgggacgtcg cgaacgactg gcgacgtcat
     2101 cgggagcgct ttcggttcgg tcgaggcctg gggggcgatc caggtcgtct ccgacgagcc
     2161 gtcactcgtc gccttggctt gcacctcgac gcgtttcggg aacggcacct tcggccagtg
     2221 cgtccccgcc ttcggcgacg aggggcggct cggcgccggg cgcagttgga cgatcgccgg
     2281 cgtccgggag gacggccact ttcggacgaa cctcatcctc gcgaacggcg cggagaccga
     2341 gaccggcgtc gacatgacgc tcctcgcgga cgacgggcgt gtcctcggct cgacgacgtt
     2401 cacgctccct cccctcggga tgacccaggt gagccgcgtc gtccgggcgc tgggcgtcgt
     2461 cggcgaggtc tccggcgcgc ggatcgtcct gtcgacccgc tcgagcggag cctcgcttgc
     2521 cgcctacgcc gccctcattg acgagacaac gaacgacccg aggacgctcc taccccggta
     2581 gctcggcgga gccccttcgc gacgaggggc cggggaccgg gtcacccccc gcttcagccg
     2641 gcgacgggga gtcgcggctc gcgccagata ctcggcagga ggccgccgca ccggaccgga
     2701 gcttcctggc ttcttcattc gatattgacg cgtggggaga gtcctatccc gcgcggtcgt
     2761 cgaacgaggc acaagtgaag ttgcgcccca cccgcttcgc catcagcgga gcgcgccgag
     2821 cgccgatggc aggagcgtct tgatgtcctc gtccttccag cgaatgccgg cacccaggaa
     2881 gaaggaccgt ctctccgggt tgatgacgtc ggtgacgccc gcccggagga agactgcacc
     2941 gcggctctgc cactggccgt agagcttcac ctgggcgtct ccgtcggcgc ggccgaaatc
     3001 ccaggcctct gcgctcagct ggagccggtc cttcaggagg tgctggtcga ttgcgaagcc
     3061 tccgcgcgac tcgatgagcc cggcgcgaag ggtggtgtcc ttgtagcggc ggccgagttg
     3121 ggcggagaga ccgaacttgt cctcggtccg cgtcaccttc gtcgtcacga cgctcggcgg
     3181 tccgccgtcc acgctcgttt cgtagcggta ggtctcatcg acccggcgcc cgttcggcag
     3241 cccgaggagc tcgatacggt agaacttccc ttctcgaggc gtcacgtcga ttcggaaggc
     3301 gccccgccac tcctcagtcc gagtcgcgta ctcgccgtag aaaccaaggt cgagatcgat
     3361 ccgattgatc cgcgtgagcg tcgtccggag ctcctcgacg cccgtcttga tcgacgtgaa
     3421 cgcctcgttc aggttgcggt gggaagtctc gtcgttcacg agcttgccga tggtcccctc
     3481 gcctttatcg atctttcccg tgatcgagtt gaggttgtcg gctgaggtct tcagtttctc
     3541 cgagagctcc tcgatgttta cgaccgagcc cttcacgctc gtccggttct cgtcgaggat
     3601 tcggtcgagc cgcgcgagcg tttcgcggat cgaggccgag aattccttca ggttcgctac
     3661 cgtcacgtcg acgttcgccc ggttcgccgc gacgaggagg cggagctcct cggtgagcgc
     3721 gccgacgttg tcgacgatcc ggttgatctt cgcctctccg gtctcgccgc cgagcgagga
     3781 tgagagggcg ccgctaatcg ctttgacgtc ccgtccgatc tcctgcgcga gtttcgtcag
     3841 ctgatcgaac tctaccggga cgctcccggg gatccgggtg ccatccggaa gacgtggctt
     3901 tgccgggtcg ccggcgtaga ggtcgacgta cttgtcgccg agaagaccga gattcttcac
     3961 ctgcccgtag gcgccgtccc gcagctcgac gtcggcgtcg aacaggagcc gggccacggc
     4021 ggtgccgtcc ggcatgagcc ggataccgtc gatgcggcca actcttacgc ccgcgatcct
     4081 gacggccgac ttgtcgtcca gccccgcgac gtccttgaac accacctcgg tcgtcgcccg
     4141 ccgggccttc cccggcaagg ggagatcctc gattttgagg acgagtgcgg ccaggacgag
     4201 cagcacggcc atcgcgaaga ggccgacttt ggcagtcgcg ttcattccga tcccttctcg
     4261 cttccggctg cctcgagctc ctcgctcgcg tcgccgcgca cgaaagcagc cacccgcgga
     4321 tcgctcgaag ctgcgaattc ccgtggcggc aggtcggcca cgatcctccc gccgtcgagg
     4381 agtgccactc gctcagccac ggcgaaggcg accttcaggt cgtgggtgat cgtcaccatc
     4441 gtcggcatca gcgtgtcccg cattcggacg atcacgcggg tgagcacgtc cgtcgtgatc
     4501 ggatcaaggc cagtcgtcgg ctcgtctaag aggaggatct ccggcttcag cgcgatggcg
     4561 cgcgcgaaac caacgcgccg ccgcatcccg cccgagagct ccgcagggcg cctctcctcg
     4621 atgccgtgaa ggcggac
//
LOCUS       DAOONL010000207         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22692_length_4583_cov_14.419611, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000207 DAOONL010000000
VERSION     DAOONL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22692_length_4583_cov_14.419611"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLP50_16750"
     CDS             <1..586
                     /locus_tag="PLP50_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53243.1"
                     /translation="EELAVPTAPPSVPTAAVLPAPTSPQASAGSRQTPVPGTMAVAPT
                     PEPQVVTPERLPDRPVQGGIDPASRLERIVPFRTGEAVPVGIVDRSITIESVTVTDWP
                     KPEELRKAEGKPGESTKLTVKFTYTNRDDEDWTCTYRVTVLDAEGREIGSGVQERSLG
                     GSEAGDTNRVSVKMRAADFARAAKLRVRILARPD"
     gene            806..1831
                     /locus_tag="PLP50_16755"
     CDS             806..1831
                     /locus_tag="PLP50_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HPA53244.1"
                     /translation="MAAAGRFARLALDCVHREYPNKIAHVMNGDADAKPPRELTPAFY
                     GCFDWHSSVHGHWLLARLARLHPDAPFAAPARAALAQSLTEANVAGEVAYLSAPGRTG
                     FERPYGLAWLLQLAAELREWDDPEAKAWSKALAPLEARAADLLFAWIPKLAYPIREGE
                     HPQTAFAFGLALDWARGAGETEKAGLLARRIVELYGADRRCPIGYEPSGQDFLSPCLG
                     EADLMRRVMPRDRFARWLSELLPDLPRDGSSGWLAPGIVTDRTDGKLVHLDGLNLSRA
                     FMLRGIAAGLPPRDRRIPALRAAAEAHAKASLPSVTSEHYEGSHWLGTFAVYLLSERG
                     LPPTLPR"
     gene            1906..2196
                     /locus_tag="PLP50_16760"
     CDS             1906..2196
                     /locus_tag="PLP50_16760"
                     /inference="COORDINATES: protein motif:HMM:NF022143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2492 family protein"
                     /protein_id="HPA53245.1"
                     /translation="MTNAAHDHHHDHSHEADGFVFGHEVLSILADRGGRLPVAELKRA
                     AAEAFGEDALYGNCHGDRFGFEGLLEFLGSAGKLSRDGDAVALGRNPGCTGH"
     gene            2237..4444
                     /locus_tag="PLP50_16765"
     CDS             2237..4444
                     /locus_tag="PLP50_16765"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPA53246.1"
                     /translation="MRPRIGLSPRTWEAGGRSFVLRRDGFGVMHAEAEDDLGLAAAWG
                     FAHAHDRALQLLVTRIVGQGRICELLDDSPSSLAIDLFMRRNLFAAASSEEAASLSGR
                     AKSLAEAYAAGVNERLARGRPWELRLLRVPSEPWTPADTLLTLRLMTFVGLAQAQGDA
                     EVALLQSLRAGVDRGKLARLFRPHLDGLDDALLGAIRALRHVPALVPGLVPLPALRAS
                     NNVAVSGRRSASGAALYASDPHLEVNRLPAIWYEVVGTLPGGDFRAGVTVPGVPGLVM
                     GRTRAIAAGFTYGFADTIDLVIEEVSGGRFRRGDAWRPLRERRETVRRKNGPPCEVVV
                     FETDAGVLETPDEASRVEDGIWVARAWTCRRAGGAASLEAILDLWAAPDIERGAEAVS
                     RVALSASWLLAGRDGRIAAQQSGLLPKRRRDPGLLPLRGWEPGDLWDGTLSPSALARV
                     AGDPDGVLVTANDGGHGAWKPVGITLCMGSDRADRLRELADAKGATGGSLSAGDLGAM
                     QADLVSGQARRLLAALDPYLPDLPAVRELRAWDRRYDAASRGAVLFERLYGALCEELF
                     GAGLFGMEAWRALRDTTLVHAFFFRLFDDLLAREEGEEWFGAEGKAAFVSRVARRALE
                     GVDPAALPPWGASRRVFLRHLLLGGKLPPALGFDRGPFEVAGGRATVAQGQLLSLSGR
                     ESAFCPSWRFVTDLADDVSETALAGGPSDRRFSRWYATDLARWLAFERKPLRP"
BASE COUNT          547 a         1739 c         1659 g          638 t
ORIGIN      
        1 cgaggagctc gccgtcccga ccgcgccgcc gtcggtgccg actgcggcgg tgctgcccgc
       61 gccgacgtcg ccgcaggcct ctgcgggatc gcgccagacg cctgtgcccg gcacgatggc
      121 cgtcgcgccg acgccggagc cgcaggtcgt gacgccggag cgccttcccg accggcccgt
      181 tcagggaggg atcgacccgg cctctcggct cgagcggatc gtccccttcc gaacgggcga
      241 ggcggtgccg gtcgggatcg tcgaccgctc gatcacgatc gagtccgtga ccgtcaccga
      301 ctggccgaag ccggaggagc tccgaaaggc cgaggggaag ccgggggagt cgacgaagct
      361 gacggtgaag ttcacctaca cgaaccggga cgacgaggac tggacctgca cgtaccgggt
      421 gacggtgctc gacgcggagg ggcgcgagat cggctccgga gtgcaggagc ggagcctcgg
      481 gggctcggag gcgggcgaca cgaaccgcgt ctcggtgaag atgcgagccg ccgacttcgc
      541 gcgcgcggcg aagctgaggg tgcggatcct ggctcgtccg gactgaagtg gatcgggggc
      601 acccgccgcc gggtgccccc gcagcgcctt cggcgcctct ccctccgcgc gcgcgagggc
      661 gggcagtgga tcgggggcac ccgccgccgg gtgcccccgc ggcgcctaga atcgcgtcgt
      721 ggttcgtctc cgcgcgatct ccttcctcct tcccgcgctg ctcgtcgcgc cggccgccgg
      781 ggcggcgggc gacgctgccc tcgacgtggc cgcggccggc cgcttcgccc gcctcgccct
      841 cgactgcgtc caccgcgagt acccgaacaa gatcgcccac gtgatgaacg gcgacgccga
      901 cgcgaagccg ccgcgcgagc tgaccccggc cttctacggc tgcttcgact ggcactcctc
      961 cgtccacgga cactggctcc tcgcgcgcct cgcgcggctc cacccggacg cgccgttcgc
     1021 ggcgccggcg cgcgcggcgc tcgcgcagag cctcacggag gcgaacgtcg cgggggaggt
     1081 cgcctacctc tccgcgccgg gacggacggg cttcgagcgg ccgtacggcc tcgcatggct
     1141 cctccagctc gcggcggagc ttcgcgaatg ggacgacccg gaagcgaagg cgtggtcaaa
     1201 ggcgctcgcg cccctcgagg cgagggccgc cgacctcctc ttcgcctgga tcccgaagct
     1261 cgcctatccg atccgggagg gagagcaccc gcagaccgcg ttcgcgttcg gcctcgccct
     1321 cgactgggcc cgcggcgcgg gcgagacgga gaaggccggg ctcctcgccc ggcggatcgt
     1381 cgagctctac ggtgccgacc ggcggtgccc gatcggctac gagccgtcgg ggcaggactt
     1441 cctctctccg tgcctcggcg aggccgacct gatgcgacgc gtgatgccgc gagaccgttt
     1501 cgcgaggtgg ctctcggagc tccttcccga cctcccgcgc gacgggtcgt cgggctggct
     1561 cgcgcccggg atcgtcaccg accggaccga cggtaagctc gtccacctcg acgggctgaa
     1621 cctctcccgg gccttcatgc tccgcggcat cgcggccggg ctcccgccgc gagaccgtcg
     1681 cattcccgcg cttcgggcgg ccgcggaggc ccacgcgaag gcgagcctcc cgagcgtaac
     1741 gtcggagcac tacgaaggga gccactggct cgggacgttc gcggtttacc tcctttccga
     1801 gaggggcctg ccgcccaccc ttccgcggtg agacgggagg atccgggcac cggcatgacg
     1861 cccgtcatcc cgggcggccg aggggcgcgt gctagggtgc gcgccatgac gaacgcggcc
     1921 cacgaccacc accacgacca ttctcacgaa gccgacggct tcgtcttcgg ccacgaggtg
     1981 ctgagcatcc tcgccgaccg gggtggccgg ctgccggtgg ccgagctgaa gcgggccgcg
     2041 gccgaggcgt tcggagagga cgcgctctac gggaactgcc acggcgaccg gttcggcttc
     2101 gagggcctcc tcgagttcct cggctcggcc ggcaagctct cgcgcgacgg cgacgcggtc
     2161 gctctcggcc ggaatcccgg ctgcacggga cactgaccgc ccggcgccgg cgggcgcatc
     2221 catagaatcc cgcggcgtgc gaccccgcat cggcctgtcg ccccggacct gggaggcggg
     2281 aggacgttca ttcgtcctcc ggcgcgacgg attcggagtg atgcacgccg aggccgagga
     2341 cgacctcggc ctcgcggcgg cgtggggatt cgcccacgcg cacgaccgcg ccctccagct
     2401 cctcgtcacg cggatcgtcg ggcagggacg gatctgcgag ctcctcgacg actcgccttc
     2461 ctcgctcgcg atcgacctct tcatgcggcg gaacctcttc gcggcagcct cgtcggagga
     2521 ggcggcctcc ctttcgggac gcgcgaagag cctggcggag gcttacgcgg cgggagtgaa
     2581 cgagcgcctc gcgcgtggac gcccctggga gctccggctc ctccgcgtcc cgtccgagcc
     2641 gtggacgccg gccgacaccc tcctgacgct ccggctcatg acgttcgtcg gcttggcgca
     2701 ggcgcagggc gacgccgagg tcgcgctcct ccagtcgctc cgggcgggcg tggaccgtgg
     2761 caagctcgcc cgcctcttcc gcccgcacct cgacggcctc gacgacgcgc tcctcggcgc
     2821 gatccgcgcg ctgcgccacg tcccggcgct cgtccccggg ctggtcccgc tcccggctct
     2881 ccgcgccagc aacaacgtcg ccgtctcggg gcgacggtcg gcctcgggcg ccgccctcta
     2941 cgcgagcgac ccgcacctcg aggtgaatcg cctgccggcc atctggtacg aggtcgtcgg
     3001 cacgctccct ggcggcgact tccgcgccgg cgtcacggtg cccggcgtcc ccggcctcgt
     3061 catggggcgg acgcgcgcga tcgcggcggg cttcacgtac ggcttcgccg acaccatcga
     3121 cctcgtgatc gaggaggtga gcggcggacg gttccgccgc ggcgacgcgt ggcgcccgct
     3181 ccgggagagg cgtgagacgg tccggcggaa gaacgggcct ccgtgcgagg tcgtcgtctt
     3241 cgagaccgac gcaggggtcc tcgagactcc ggacgaggcg agccgcgtgg aggacggcat
     3301 ctgggtcgcc cgcgcctgga cctgccggcg ggcgggcgga gccgcatctc tcgaggcgat
     3361 cctcgacctc tgggcggcgc cggacatcga gcgcggcgcc gaggccgttt cccgcgtcgc
     3421 gctcagcgcg agctggcttc tcgcgggccg cgacggccgg atcgcggcgc agcagtcggg
     3481 actcctcccg aagcgccgcc gcgatccggg cctcctcccg ctccgcggct gggagccggg
     3541 cgacctctgg gacggcacgc tttcgccctc ggccctcgcc cgcgtcgccg gcgatccgga
     3601 cggggtcctc gtcaccgcca acgacggcgg ccacggcgcg tggaagccgg tcgggatcac
     3661 gctctgcatg ggctccgacc gcgccgatcg ccttcgcgag ctcgccgacg cgaaaggcgc
     3721 cacgggggga agcctctccg ccggcgatct cggcgcgatg caggcggacc tcgtctccgg
     3781 gcaggcgcgg cggcttctcg ccgccctcga cccgtacctc cccgacctcc ccgcggtccg
     3841 cgagcttcgc gcgtgggacc ggcgctatga cgccgcctcg agaggggccg tcctcttcga
     3901 gaggctctac ggcgcgctct gcgaggagct cttcggcgcg gggctcttcg ggatggaggc
     3961 gtggcgggcg ctgcgcgaca cgacgctcgt ccacgccttc ttcttccgcc tcttcgacga
     4021 cctcctcgcg cgcgaggaag gggaggagtg gttcggcgcg gaagggaagg cggcgttcgt
     4081 ctcccgggtc gcccgccggg ccctcgaggg ggtcgatccc gccgcgcttc cgccgtgggg
     4141 agcgtcccgg cgcgtcttcc tccggcacct cctcctcggc gggaagctcc cgccagccct
     4201 cggcttcgac cgtgggccgt tcgaggtcgc gggcggccgt gcgacggtcg cccagggaca
     4261 gctcctctcg ctctcggggc gcgagtcggc cttctgtcct tcctggcggt tcgtgaccga
     4321 cctggcggac gacgtctccg agaccgccct cgccggcggc ccctcggacc gccgcttctc
     4381 ccgctggtac gcgaccgacc tcgcccgctg gctcgccttc gagcgcaagc ccctccgccc
     4441 ctgatccctc cggggacggg gacggtgccg acggggacgg tgcccgacgg acggggacgg
     4501 tgccaggcgt gaaatcgtgt attgtttcaa caatacacga tttcacgcct ggcacctcgg
     4561 gcgggccctc gggcgggcgc ccg
//
LOCUS       DAOONL010000208         4568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22788_length_4568_cov_16.863284, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000208 DAOONL010000000
VERSION     DAOONL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22788_length_4568_cov_16.863284"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLP50_16770"
     CDS             complement(<1..947)
                     /locus_tag="PLP50_16770"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPA53247.1"
                     /translation="MPRTHRRLASALLAFTLLAAPAAVLLAEKGAAPAAPARRTVAVT
                     IDDLPFVAYGLPLEAVRTLSKELVAALAARKVPAVAFVNEDRVFVRGEVDERIALLEE
                     WLDAGMELGNHTWGHPGLTRTPLLRMQDAVVRGEVVTRLLLERRGKALRWFRHPYTQT
                     GATKETKEAFEAFLAGRGYAVAPFSIEHEDWVFASADAKLAAERDEAGRARLLEAYLT
                     NFEARVAFYEERSRALFGREIPQIHLAHANALNARAMPHLLERLEKRGYAFVTLDEAL
                     ADPAWKSPDGYVGKWGPSWLHRFAAARGEDVGALFRAEP"
     gene            1038..1958
                     /locus_tag="PLP50_16775"
     CDS             1038..1958
                     /locus_tag="PLP50_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPA53248.1"
                     /translation="MARILVSGLVNLETTVKVEAFPVPYFPVRYPFFGVASSVSGVGY
                     NVARALQALGNAPLLLSLVAPDLAGRQVRDALSRDGLDGFWVRDGARETAQSVVLYDG
                     QGRRQIHVDLKDVQERAYPQELFVQALAASDAAALCNVNFSRPFLAAARAAGKLVATD
                     VHALASLDDPYNGDFLRAAQVVFLSDDALPAAPEEFARELGARFSPEVVVVGMAARGA
                     LLHLPSEGIVERVPAVRTRSVVSTVGAGDALFSAFLDGYLRERDARAALRRAAVFASW
                     KLGAASAAEGFLGAEPLRQLVASDPDPFEP"
     gene            2040..3128
                     /locus_tag="PLP50_16780"
     CDS             2040..3128
                     /locus_tag="PLP50_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53249.1"
                     /translation="MKILTGTLVVLCLLVPASRAGAQAIVSDHTTTDVAAIPDAAIAN
                     AAALRLLVRHASVGGNIDSGLDALHTANARYDRSLWDFQSRGNPGWQAKVDDLVAQAA
                     AQGSSFDVLTMKFCYIDPNASFTYYRDALLGLEATYPTKRFVWWTIPIETSGNTNRQA
                     FNDLVRAYAAANGKVLFDVADIESHNAAGVKRVDGSGRELMWDEWTSDGGHLSAAGAQ
                     RVASALWWLMARIAGWSPTADPLSFHTLVPCRIVDTRNATGPYGGPPLAAGAARAFTI
                     AGQCGVPADAAAVALNVTVTNPTSAGSLTVYPGTGTVPGTSTISFAAGKTRANNTTLG
                     LVGGVLSVLDRQETGTTHVIVDVSGYYR"
     gene            complement(3205..4374)
                     /locus_tag="PLP50_16785"
     CDS             complement(3205..4374)
                     /locus_tag="PLP50_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPA53250.1"
                     /translation="MDRVVDFAALSPEDDRTPLVCSLCDAIEATWDEGLYPLVAVDRP
                     WSRHNPLLRGEFARPRAIRWYLERELGKLASAGAKITVGGSRERVPFDDPAHFESLDE
                     EAWDLRQKKLFLFRAERIDLSLNRLEHYTGTPAERFQRHVLFTNYEMHAEAFRARYPG
                     AVGPGRSVQMPAFHHELPGNAGLTLVNIGVGPSNAKTITDHVAVLRPDAVLMVGHCAG
                     LRNHQEVGDLVLATGYMRDDRILDDVLPTSVPVASNHFLNALLLEEMEARGLSYRLGT
                     VFTTSNRNWEFNQSGALAAIRASRSVAVDMESATVAANGFRYRIPTAALLCVSDKPLH
                     GQPKLSAEAQSFYRESRVTHLEVAAEALEKARDRFPGGLPASDIRSSDEPLLGSS"
BASE COUNT          643 a         1600 c         1671 g          654 t
ORIGIN      
        1 tcgggctcgg cccggaagag ggcgcccacg tcctcgcccc gggccgccgc gaagcggtgg
       61 agccaggacg ggccccactt gccgacgtac ccgtcggggc tcttccaggc ggggtcggcg
      121 agcgcctcgt cgagcgtgac gaacgcgtag cctcgcttct cgagacgctc gaggaggtgc
      181 ggcatcgccc gggcgttcag ggcgttcgcg tgcgcgaggt ggatctgcgg gatctcgcgg
      241 ccgaagagcg cgcgcgagcg ctcctcgtag aaggcgacgc gcgcctcgaa gttcgtaagg
      301 tacgcctcga ggaggcgcgc gcggccggcc tcgtccctct ccgcggcgag cttcgcgtcg
      361 gccgaggcga agacccagtc ctcgtgctcg atgctgaagg gagcgacggc gtagccccgc
      421 ccggcgagga acgcctcgaa ggcttccttg gtctccttcg tggcccccgt ctgcgtgtac
      481 gggtgacgga accagcggag cgccttcccc cgcctctcca ggaggaggcg ggtgacgacc
      541 tctccccgga cgacggcgtc ctgcatccgg agcagcggcg tgcgcgtcag gccggggtgg
      601 ccccaggtgt ggttcccgag ctccatcccg gcgtcgagcc actcctcgag gagggcgatc
      661 cgctcgtcca cctcgccgcg gacgaagacg cggtcctcgt tgacgaacgc gacggccggg
      721 accttccgcg cggcgagcgc cgcgacgagc tccttcgaga gcgtccggac cgcctccagc
      781 gggaggccgt aggcgacgaa cgggaggtcg tcgatcgtga cggcgacggt ccggcgcgcg
      841 ggggcggccg gagcggcgcc cttctccgcg aggaggacgg cggccggcgc ggcgagcagc
      901 gtgaaggcga ggagggcgga tgcgaggcgg cgatgcgtac ggggcacggg aacctccggg
      961 tcgcgagatg gggtcgccgc gcattgtgca cacgtgccgt ccgcggcaaa gggcaccgtg
     1021 gagcagaatg gggcgtcatg gcacgaatcc tcgtctccgg cctcgtgaac ctcgagacga
     1081 ccgtgaaggt cgaagcgttc ccggtcccgt acttcccggt ccgctacccg ttcttcggcg
     1141 tcgcctcctc ggtctccggc gtcggctaca acgtcgcccg ggcgctccag gctctcggga
     1201 acgcgcccct tctcctctcg ctcgtcgccc ccgacctcgc ggggcgccag gtgcgcgacg
     1261 ccctgtcgcg ggacggcctc gacggcttct gggtccgcga cggggcccgc gagacggcgc
     1321 agtcggtcgt cctctacgac ggtcaggggc gacgccagat ccacgtcgac ctgaaggatg
     1381 tccaggagcg cgcctacccg caggagctct tcgtccaggc gctcgccgcc tccgacgcgg
     1441 cggccctctg caacgtcaac ttctcgcgcc ccttcctcgc cgcggctcga gccgcgggca
     1501 agctcgtcgc gaccgatgtc cacgccctgg cctctcttga cgacccgtac aacggcgact
     1561 tccttcgcgc cgcgcaggtc gtcttcctga gcgacgacgc gctcccggcg gcgcccgagg
     1621 agttcgcccg cgagctcggg gcgaggttct ccccggaggt cgtcgtcgtc gggatggcgg
     1681 cgaggggcgc cctcctccac ctcccgtccg aagggatcgt cgagagggtc ccggccgtgc
     1741 ggacacgctc cgtcgtctcg acggtcggcg cgggcgacgc gctcttctcg gcgttcctgg
     1801 acggctacct gagggagcgc gacgcgcggg cggcgctccg gcgcgccgcc gtcttcgcct
     1861 cgtggaagct cggggccgct tccgcggcgg agggcttcct cggggccgag ccgcttcggc
     1921 agctcgtcgc ctccgatccg gacccgttcg agccgtagaa gagcggatgc gcccggggag
     1981 gcgtgcttgc atgccaacac gttcgggtcg atagtcccgg agccggccgg agagacagca
     2041 tgaagatcct cacgggtacc ctcgtcgtcc tctgcctgct cgtccccgcc tcgcgggcgg
     2101 gcgcccaggc gatcgtctcc gaccacacca cgacggacgt cgccgcgatc cccgacgcgg
     2161 cgatcgcgaa cgcggccgcg ctccgcctcc tcgtccgcca cgcctccgtc ggcgggaaca
     2221 tcgactcggg actcgacgcc ctccacacgg cgaacgcccg gtacgaccgg agcctctggg
     2281 acttccagag ccgcgggaac ccgggctggc aggcgaaggt cgacgatctc gtggcgcagg
     2341 ccgccgcgca ggggtcgagc ttcgacgtcc tgacgatgaa gttctgctac atcgacccga
     2401 acgcgagctt cacctactac cgcgacgcgc tcctcgggct cgaggcgacg tacccgacga
     2461 aacgcttcgt ctggtggacg atcccgatcg agacgtcggg gaacacgaac cgccaggcgt
     2521 tcaacgacct cgtgcgggcg tacgccgccg cgaacgggaa ggtgctcttc gacgtcgccg
     2581 acatcgagag ccataacgcc gcgggcgtga agcgggtcga cggcagcggc cgcgagctga
     2641 tgtgggacga gtggacctcc gacggcgggc acctgagcgc cgccggcgcg cagcgcgtcg
     2701 ccagcgcgct ctggtggctc atggcccgga tcgccggctg gagcccgacg gccgacccgc
     2761 tctctttcca taccctggtc ccctgccgga tcgttgatac ccggaacgcg accggcccct
     2821 acggcggccc gccgctcgcc gcgggcgcgg cgagggcgtt cacgatcgcg ggccaatgcg
     2881 gcgtcccggc cgacgcggcg gccgttgctc tgaacgtcac cgtcacgaac ccgacgtcgg
     2941 cggggagcct gacggtctac cccgggacgg ggaccgtacc ggggacgagc acgatctcct
     3001 tcgcggcggg gaagacgcgc gcgaacaaca cgacgctcgg cctcgtcggc ggcgtcctgt
     3061 cggtcctcga ccgacaggag accgggacga cgcacgtcat cgtcgacgtg agcggctact
     3121 atcgctgagg gcgaacgagg ggaaggcgtg tccatcgcgc gctgcggccg tctcggccgg
     3181 agcgcgcaac cgccgccgtc gccctcagct cgaaccgagg agaggctcgt ccgaggagcg
     3241 gatgtcggag gcggggaggc cgccggggaa gcggtcccgc gccttctcga gcgcctcggc
     3301 ggcgacctcg aggtgggtga cccggctctc gcgatagaac gactgcgcct cggcggagag
     3361 cttcggttgg ccgtggagcg gcttgtcgct gacgcagagg agggcggccg tcgggatccg
     3421 gtagcggaag ccgttcgcgg cgacggtcgc cgactccatg tcgacggcga cgctccggct
     3481 cgcacggatc gcggcgaggg cgcccgactg gttgaactcc cagttccggt tcgaggtcgt
     3541 gaagaccgtc ccgagccggt aggagaggcc gcgcgcctcc atctcctcga ggaggagcgc
     3601 gttcaggaag tggttcgagg cgaccgggac gctcgtcggc aggacgtcgt cgaggatccg
     3661 gtcgtcgcgc atgtagccgg tggcgaggac gaggtcgccg acctcctggt ggtttcgcag
     3721 gcccgcgcag tgacccacca tcaggacggc gtcggggcgg aggaccgcga cgtggtcggt
     3781 gatcgtcttg gcgtttgagg gcccgacgcc gatgttcacg agcgtcaacc ccgcgttgcc
     3841 ggggagctcg tggtggaagg ccggcatctg gacgctccgc cccggaccga cggcgccggg
     3901 gtagcgggcg cggaacgcct cggcgtgcat ctcgtagttc gtgaacagga cgtgccgctg
     3961 gaacctctcc gcgggggtgc cggtgtagtg ctcgaggcgg ttcagggaga ggtcgatccg
     4021 ctcggcgcgg aagaggaaga gcttcttctg gcggaggtcc cacgcctcct cgtcgaggct
     4081 ctcgaaatgg gccgggtcgt cgaacgggac cctctcccgc gacccgccga cggtgatctt
     4141 cgcgccggcc gaagcgagct ttccgagctc ccgctcgaga taccaccgga tcgcccgcgg
     4201 ccgtgcgaac tcgccgcgga ggagggggtt gtggcgcgac caggggcggt ccaccgcgac
     4261 gagcgggtag agcccctcgt cccaggtcgc ctcgatcgcg tcgcagagcg agcagacgag
     4321 cggagtccga tcgtcctcgg gggagagggc cgcgaaatcg acgacacgat ccatgaaacg
     4381 tccagattac cggagacggc gtctccgtgc attctcatgt cgccggtcgc gcgccggcac
     4441 gccgccctcg ggaggagtag gattcctccc ccttccgacc ggaaggaata ccaaggaggc
     4501 acatgtccaa gcgtttttcg gacctcgatc aaaggaggcg ggagcttctt ctgggcggcg
     4561 gggccgag
//
LOCUS       DAOONL010000209         4558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22868_length_4558_cov_8.421275, whole genome shotgun sequence.
ACCESSION   DAOONL010000209 DAOONL010000000
VERSION     DAOONL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22868_length_4558_cov_8.421275"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..220)
                     /locus_tag="PLP50_16790"
     CDS             complement(11..220)
                     /locus_tag="PLP50_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53251.1"
                     /translation="MTMATTYEVASSGGDLATPEEVATAFPCVRCSAPAGPSALLCPA
                     CYAEKRPPKCPACGGRLVDGKCGWC"
     gene            complement(463..2622)
                     /locus_tag="PLP50_16795"
     CDS             complement(463..2622)
                     /locus_tag="PLP50_16795"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HPA53252.1"
                     /translation="MQQCREAPHRRARPAWLPRRYYRPRRHRVVVDSVPRSERRHSHR
                     AGVGHLGARCGPAARWRRNPRDARGEVRPAPIVTPGPHRVRRTPHPLPSPRDGKLSNS
                     SGRLGAGLDVRGDGGYIVGPPSRHASGGEYVLLDEDESVLEPAPNWLLELIGERYTEA
                     ESDEPVEELPDDQAELVARRKLARAIVRVREGYSRHQTAVWMFQQCRDNRIPRGVARD
                     LIDPFLLVCAEKKDRAVPRPEVMKAFAWAYKKAPREADKEIAVRIGGKTILEIAAIPS
                     DIERHKAIKAVAAAEAVPVAAVKAEVARQRAAVAVPAPTDWRAMLLWRETPSGRKLET
                     CLRNAAVFLTHHEQWRGLIKANQFSGAVEWVPGDQPPIEAPAGRWSDIHTFQSTEWLQ
                     AGPRLMVGSDIVYSAIQLAAQAAAMHPVREYLEKLVWDQEPRLDSWLEDLAGVTRSPH
                     ASAVGRRWLVSACARAIRPGSKADHALILEGAQGIGKSTLLRTLFEPWYTDEIDVLGS
                     KDAAMQIRGAWCIEMSELDALHRSDVSRVKAFISRTVDRYRPPYGREVIEAPRGCVFA
                     GSVNDSQYLRDETGGRRFWPVACTEIDIEGAKATRDQLWAEAMVAFRAGEAWWLDTRD
                     LVEKAEEEQERRYFADAWEEQIAEALQPYKWTAGQPVTIANVMKAIGIDTARQDRGSQ
                     MRVAGCLKRLGRELVKTKQDGEWLRLWMPKGWKRGDQ"
     gene            complement(2711..3055)
                     /locus_tag="PLP50_16800"
     CDS             complement(2711..3055)
                     /locus_tag="PLP50_16800"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HPA53253.1"
                     /translation="MPILAKSGNDLNAEILIAWGAHPSVRLWRANAGQAWVPTKDGAR
                     PVQMNVPGCADLIGILGPHGRFLAIETKSANDDMRETQRRFRTMIEARGGLYIVARSV
                     EDVDRVLGPLCY"
     gene            complement(3030..4397)
                     /locus_tag="PLP50_16805"
     CDS             complement(3030..4397)
                     /locus_tag="PLP50_16805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA53254.1"
                     /translation="MELRPYQLAAVDQARALTMRGVRRVCIVAPTGAGKSVIAAHIAA
                     SAAAKRNRVLFLAHRLELIEQLSDKLDAVGLDHGVIQGDHPRRNDLPIQVASVATLIR
                     RKLPPADVVMVDECHRAPSESFRRILKAYAGTILGFTATPYRLDGRGLAEFFDGCVNV
                     AHVDELIAAGWLVPPIVIAPEEPDVSMVRRVAGDFDPSQLADLMNRPVLIGNAVETWM
                     KRAQGRTSVYFATSIEHSLNVVAAFREAGIPAEHIDGTTPLPERAAILSRAASGETIV
                     LCNVGILGEGWDLPRVSCVGQLRPTQSRALYRQMVGRGLRTWGGKRDCIVLDHAGNVY
                     RHGLPTERDEPGLSGAKRRDASRALSVRQCPLCYACAPGTAFVCPACAQPFPVRHRPL
                     IATKAGELGEVTWKERATPDRRAELFEKWGRIERERGYKPGYARAIYKSTFGVWPDAD
                     TCQVG"
BASE COUNT          676 a         1561 c         1555 g          766 t
ORIGIN      
        1 ggccaggcca tcagcaccac ccgcactttc cgtcgaccag gcggccgccg caggccgggc
       61 acttcggcgg tcgcttctcc gcgtagcagg ccgggcagag gagggcggaa gggcctgccg
      121 gggcggagca gcgcacgcag gggaacgcgg tggcgacctc ttccggcgtg gcgaggtcgc
      181 caccggagga cgccacctcg taagtagttg ccatcgtcat ctttacctcc ttattggcga
      241 ccttggcgac cttggcgact tgtttgaaag ggttatggga aatgtcgtcg ccacacggat
      301 ctgcaccaga cacgtacggg aaatcgacga cacgcggaaa tctccagagc cctcttgggg
      361 caagtagcgc caacggtcgc caagtcgcca acgcgggcat aactcgtttt ggatcagacg
      421 cataggtgtg gcgaccttgg caggcgacct ccgcaggcga cctcactggt cacccctttt
      481 ccaccccttc ggcatccaga ggcgtagcca ttcgccgtcc tgtttcgtct tcacgagctc
      541 cctgccgagc cgtttcagac agccagctac gcgcatctgc gaccctcggt cctgccgcgc
      601 ggtgtcgatt ccgatcgcct tcatcacgtt cgcgatggtc accggctgcc cggccgtcca
      661 cttgtagggc tggagagcct ccgcgatctg ctcctcccag gcgtccgcga agtaccgccg
      721 ctcctgctcc tcctcggcct tctcgacgag gtcccgtgtg tccagccacc aggcctcgcc
      781 ggcgcggaag gcgaccatcg cttccgccca gagctggtcg cgggtcgcct tggccccctc
      841 gatgtcgatc tcggtgcagg ccaccggcca gaatcgccgg ccgcccgttt cgtccctgag
      901 gtactgcgag tcgttcacgg atcctgcgaa gacgcagccg cgaggagcct cgatcacctc
      961 ccgtccgtag ggcgggcggt agcgatcgac ggtccgggag atgaacgcct tcaccctgga
     1021 cacgtcggag cggtggaggg cgtcgagctc ggacatctcg atgcaccagg ccccgcggat
     1081 ctgcatcgcg gcgtccttgg acccgaggac gtcgatctcg tccgtgtacc agggctcgaa
     1141 cagcgtgcgg agcagggtcg acttcccgat gccctgcgct ccctcgagaa tcagggcgtg
     1201 gtctgccttg ctccccgggc ggatggcccg cgcgcacgcg gagaccagcc agcgtcgacc
     1261 gacggccgag gcgtgaggcg accgtgtaac gcccgcgagg tcttcgagcc acgagtccag
     1321 tcggggctcc tggtcccaca cgagcttttc gaggtactcc ctgacgggat gcatcgccgc
     1381 cgcctgggcg gcgagctgga ttgccgagta gacgatgtcc gacccgacca tgagtcgggg
     1441 ccccgcctga agccactctg tcgactggaa cgtgtggatg tccgaccagc gtcccgccgg
     1501 tgcttcgatg ggcggctgat cgccagggac ccattcgacg gcgcccgaga actggttcgc
     1561 cttgatgagc cccctccact gctcgtggtg agtcaggaat acggccgcgt tccggaggca
     1621 ggtctcgagc ttcctgccac tcggagtctc ccgccagagc agcatcgccc tccagtcggt
     1681 cggagctggg acggctacgg ccgcccgctg cctggcgact tctgccttca cggccgccac
     1741 cggaacggct tcggcggccg cgacggcctt gatcgccttg tggcgttcga tgtcggaagg
     1801 gatggcggcg atctcgagga tcgttttccc gccgatcctg accgcgattt ccttgtcggc
     1861 ctcgcgcggg gccttcttgt acgcccaggc gaaggccttc atcacctctg gtcggggaac
     1921 ggcccggtcc ttcttttcag cgcagacgag gaggaatgga tcgatcaggt cgcgcgccac
     1981 accccgcggg attctgttgt cgcggcactg ctggaacatc cagacggccg tctggtgccg
     2041 ggagtagccc tcccgcactc ggacaatggc gcgggccagc ttccggcgtg caaccagctc
     2101 ggcctgatcg tccgggagct cctcgacggg ctcatcgctt tccgcttccg tgtagcgctc
     2161 gccgatcagc tcgaggagcc agttcggggc gggctcgaga actgattcgt cctcgtctag
     2221 gagcacgtac tccccgcccg aggcatgacg tgatggtggg ccgacgatgt acccgccgtc
     2281 gccgcgcacg tcgaggccgg ctccgagtcg accggacgag ttggagagct tcccatcccg
     2341 gggtgacgga agaggatgtg gagtcctccg gacccggtgc gggcccggag tgacgatggg
     2401 agccggccgt acttcgcctc gagcgtctcg agggttacgt cgccaccgtg ccgcgggtcc
     2461 acatcgagcg cccagatgcc cgactcctgc ccggtggcta tgccgacgtt cgcttcgggg
     2521 aaccgagtcc accacgactc gatgacggcg gggtcggtag tagcgtcgtg gcagccatgc
     2581 gggacgagct cgccgatggg gtgcttcgcg gcattgctgc atgggttgtt cccgcacgcg
     2641 cagccggcgg gggtcggcca ccataggggc aggacgcgcc agccgcgggc ggcgtgggag
     2701 agggcggcgt ttagtagcac agcggcccca gtacccggtc cacgtcctcg acggagcggg
     2761 cgacgatgta gagaccgcca cgcgcctcga tcatggttcg gaagcgccgc tgggtctcgc
     2821 gcatatcgtc gttcgccgac ttcgtttcga tggcgaggaa gcggccgtgc gggccaagga
     2881 tcccgatcag atccgcgcac cccggcacgt tcatctgcac gggacgcgcg ccgtccttgg
     2941 tcggaaccca cgcctgcccg gcgttcgcgc gccacagcct cacagatggg tgcgcgcccc
     3001 acgcgatcag gatctcggcg ttgaggtcgt tacccgactt ggcaagtatc ggcatctggc
     3061 cacactccga acgtgctctt gtagatcgcc cgcgcgtagc ccggcttgta gccgcgctcg
     3121 cgttcgatcc gtccccactt ctcgaagagc tccgcacggc ggtccggggt ggcgcgttcc
     3181 ttccacgtca cctccccaag ctctccagcc ttcgtcgcga tcagcggccg gtgccgcacc
     3241 gggaagggct gcgcgcacgc ggggcaaacg aacgccgttc cgggcgcgca cgcgtagcag
     3301 aggggacact gacggacgga gagggcgcgt gaggcgtcac gcctctttgc cccggagagt
     3361 ccaggctcat cccgttcggt tggcagaccg tgccggtaga cgttgccggc gtggtcgagg
     3421 acgatgcagt ctcgcttgcc gccccacgtt cggagccccc gtcccaccat ctggcggtac
     3481 agcgcccggc tctgtgtcgg gcgaagctgg ccgacgcacg acacgcgcgg gaggtcccag
     3541 ccctcgccga ggatgccgac gttgcagaga acgatggtct cgccagaggc ggccctcgag
     3601 aggatcgcgg cgcgctcggg gagaggcgtc gtgccgtcga tgtgctcggc gggaatgccg
     3661 gcctcgcgga aggcggcgac gacgttcagc gagtgctcga tcgaggtggc gaagtagacc
     3721 gacgtccgcc cctgcgctct cttcatccac gtctcgacgg cgttcccgat caggaccggg
     3781 cggttcatca ggtccgcgag ctgcgacggg tcgaagtcgc cagccacccg gcgcaccatc
     3841 gacacgtcag gctcttctgg ggcgatgacg atcggcggaa ccagccaccc ggcggcgatc
     3901 agctcgtcga cgtgcgcgac gttcacgcac ccgtcgaaga actcggccag gccgcggcca
     3961 tccagcctgt acggcgtggc agtgaagccg aggatcgtcc cggcgtacgc cttcagaatc
     4021 cgccggaacg actcggacgg tgcgcggtgg cactcgtcca ccatgaccac gtcggccggc
     4081 gggagcttcc tgcggatgag cgtcgcgacc gacgcgacct gaatcggcag gtcgtttcgg
     4141 cgcgggtggt ccccctggat tacgccgtgg tccagtccga ccgcgtcgag cttgtcggac
     4201 agctgctcga tcagctcgag gcggtgcgcg aggaagagga cgcggttcct cttcgccgcc
     4261 gcgctcgcgg cgatgtgggc cgcgatgacc gacttcccgg cacccgtcgg ggcgacgatg
     4321 cagacgcgac ggacgccgcg catggtgagc gcgcgagcct ggtcgactgc ggcgagctga
     4381 tacggcctca gctccacagc tccccctgcc cctgggcctc gagttcctcg cgctcgatcc
     4441 gctcgcggta cttgacgagc gccccgcacg cctcggggta gctggcgtgg cgggagatga
     4501 cttggtcggt ggggcggacg agggcgtgat gctcgacgag ggcggtgccg gcgggggt
//
LOCUS       DAOONL010000210         4546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_22958_length_4546_cov_10.793587, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000210 DAOONL010000000
VERSION     DAOONL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_4546_cov_10.793587"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1636)
                     /locus_tag="PLP50_16810"
     CDS             complement(35..1636)
                     /locus_tag="PLP50_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HPA53255.1"
                     /translation="MSAIPDNMLRLQREATLALVQTGEELPWPERQPIPPSRSAAPSM
                     PPDLLPVAFRDWIADDAERLGACIEFVAVPAVISAGSLLGRSLSIRPKARNNWTVIPN
                     LWGQSIARPAGMKTPTANSGLYHLRRLAAEEMDRYKRVRADAKARLDVLAAQRAALLR
                     AAKKTNADTLAADVAALDRQIEEVRPVAARRRYIVNDCTVEALQSILSENRSGVFWYR
                     DELTGLYGMMGRDGHENDRAFLLEAYEGGLSGAYESDRIARGNVRADGPCVSLFGTIQ
                     PGPIQRQVEGAVAGRGGADGWIQRFGMTVFPDEPGEGRGVDREPNVKARDLAFRAFRF
                     LDEADRVRDLGAQQEDGAPDFLRFAPDAQGIFDDWLEEHHDAVRNAGDTPAFEEYLVK
                     QRKTVPALALIFHALDVAVGAERPGPVSARAVDLATQWGAFLRLHAEKLFSLELRQAE
                     TAAAALGARLLRRAVPDGLTVSDLGERDWSGLGRPALVHAALDVLEPLGWIRRDYQIT
                     SGRTRTVVRIHPDFRTPAAAPEAPE"
     gene            complement(1633..1857)
                     /locus_tag="PLP50_16815"
     CDS             complement(1633..1857)
                     /locus_tag="PLP50_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53256.1"
                     /translation="MTPATEALLAFDVDEVLADAKRAAARRTPEERARHIAALGRARI
                     EAARYLLAHGNPSDRRLAARFAFGMTGRAA"
     gene            complement(2053..2325)
                     /locus_tag="PLP50_16820"
     CDS             complement(2053..2325)
                     /locus_tag="PLP50_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53257.1"
                     /translation="MPKPNKSGAAPRTRPRPDLVLALRRTDAATALGWTEGYLRQREA
                     DGTAPPHVRTGKVVIYPLNRLEEWIQRNTIDPSVIGPRPVGSRPAA"
     gene            complement(3193..3396)
                     /locus_tag="PLP50_16825"
     CDS             complement(3193..3396)
                     /locus_tag="PLP50_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53258.1"
                     /translation="MSSIKVNPGDTYQAHVGGNGAAGIVAVAITVHRPTGRVVEEWTA
                     EDFARAKGRRKRLDRFGQEMKEA"
     gene            complement(3580..>4546)
                     /locus_tag="PLP50_16830"
     CDS             complement(3580..>4546)
                     /locus_tag="PLP50_16830"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPA53259.1"
                     /translation="GLTDVGRKRKHNEDAYLLDAERGLFVVADGMGGHAAGEVASRLT
                     VESIQEFIAGTEDDHDNTWPFGYNNRYSVDGNRLSTAVERANEKVMRAVVNRPELKGM
                     GTTVVAALFDEKRATLVHVGDSRAYLYRDSELRRLTDDHSWVQEQVNAGILSEEEARS
                     HPLKNVVTRALGGGAHVAVDLIEIPVGDGDRFLLCSDGLTGMVSDEEITEALSSARSL
                     EQIVRGLIDLANERGGVDNITAITVEVRNGEARKADTSREKTSVSLRVAKVDDEDLTP
                     TDEIPVPVVESPPETPPAGLPAKDEEPPAPAGGEAAGEPGSEPKG"
BASE COUNT          573 a         1529 c         1693 g          751 t
ORIGIN      
        1 ggtcgggtcc gccagcgggg gacgaagacg ccgctcattc gggggcctcc ggggccgctg
       61 cgggagtccg gaagtccggg tgaatccgaa cgaccgttcg ggttcggccg gaggtgatct
      121 ggtagtcgcg cctgatccag ccgaggggtt cgaggacgtc gagcgcggca tggacgagtg
      181 cgggccggcc gagccctgac cagtcgcgct ccccgaggtc cgagacggtg aggccgtcgg
      241 ggacggcgcg gcggagtaga cgggcgccga gggcggcggc ggcggtctcc gcctgtcgga
      301 gctcgagcga gaagagcttc tcggcgtgga ggcggaggaa cgctccccac tgagtcgcca
      361 ggtcgacggc gcgggccgag acggggccgg ggcgctctgc cccgaccgcg acgtcgagcg
      421 cgtggaagat gagcgcgagg gcggggaccg tcttccgctg cttgacgagg tactcctcga
      481 atgctggggt atcaccggcg ttgcggacgg cgtcgtgatg ctcctcgagc cagtcgtcga
      541 agatgccctg agcgtcgggg gcgaaccgga gaaagtccgg ggcgccgtct tcctgctggg
      601 cgccgaggtc ccggacgcgg tcggcttcgt cgaggaaccg gaaggcgcgg aatgccaggt
      661 cgcgggcctt cacgttcggt tccctgtcga cgccgcgacc ctcgccgggc tcgtcgggga
      721 agacggtcat gccgaaccgc tgaatccatc cgtcggcgcc tccgcgtccg gctacggcac
      781 cttctacttg ccgctggatc gggccgggct ggatcgtgcc gaagagggac acacacgggc
      841 catcggcgcg cacgttgccg cgggcgatcc ggtcggattc gtaggcgccg gagagtcctc
      901 cttcgtaggc ttcgaggagg aacgcgcggt cgttctcgtg tccgtcgcgg cccatcatcc
      961 cgtagaggcc ggtcagctcg tcccgatacc agaagacgcc gctccggttc tccgagagga
     1021 tgctctggag cgcctcgacg gtgcagtcgt tcacgatgta gcggcgccgg gcggcgacgg
     1081 gccgaacctc ttcgatctgg cggtcgagcg ccgcgacgtc ggcggcgagg gtgtcggcgt
     1141 tcgtcttctt cgccgcgcgg aggagggcgg cccgctgcgc cgcgaggacg tcgagccggg
     1201 ccttggcgtc ggcgcggacg cgcttgtagc ggtccatctc ctcggcggcg agacgacgga
     1261 ggtggtagag gccggagttc gccgtcgggg tcttcatgcc ggcgggccgc gcgatgctct
     1321 gcccccagag gttcgggatc actgtccagt tgttccgcgc cttcggccgg atgctgaggg
     1381 agcggccgag gagggagccg gcggagatga cggcggggac ggcgacgaat tcgatgcagg
     1441 cgccgagtcg ctcggcgtcg tcggcaatcc agtcgcggaa cgcgaccggg aggaggtccg
     1501 ggggcatgga gggcgcggcc gaccgcgacg gcgggatcgg ctgccgctcg ggccagggga
     1561 gttcctcgcc cgtctggacg agggccagcg ttgcctctcg ctggagacgg agcatgttgt
     1621 cggggatggc gctcatgctg ctctcccggt catcccgaac gcgaaccgtg cggcgagacg
     1681 gcgatccgag gggttcccgt gcgcgaggag gtagcgggcg gcctcgatcc gggcgcggcc
     1741 gagggccgcg atgtggcggg cgcgctcctc cggggtacgg cgggcggcgg cgcgtttcgc
     1801 gtccgcgagg acctcgtcca cgtcgaacgc gaggagggcc tcggtcgcgg gcgtcatgcc
     1861 gccccccgag ccgcccagcg ggcgagccgg ccgatcgcga gggagcggtc gacctggacg
     1921 ggtctggagt tacgatcggt cggcgcggta gaatgcagcc tgcaagcggt tttcgatcgc
     1981 gtcggccggt tcgggtggtt cagacccgag ccggcctctt ctttttcgct catggtggtt
     2041 ccttccgctt cctcaggccg ccggccggct tccgacgggc cgcgggccga tgacgctcgg
     2101 gtcgatcgtg tttcgctgaa tccactcctc gaggcggttg agcgggtaga tgacgacctt
     2161 ccccgtccgg acgtgcggcg gcgccgtccc gtcggcctcg cgctgcctga ggtatccctc
     2221 cgtccagccg agcgccgtcg cggcgtcggt ccgccgaagg gcgaggacga ggtcgggccg
     2281 cggcctggtt cgcggggccg ctccgctctt gttgggcttg ggcatcggtc gtgccctcct
     2341 ttcgctgcaa ggatcggttt gcacgggcct ttcgtgaagg ctccacggca acggattggg
     2401 ggtctctaac tgttgccttc acggtgtcga cgaagcgcga gaccttcttc gatctcctgt
     2461 tccacgcgtt gagcagcggc ttgaaggtgc ctcggatgtg gtgccacttc ctccacgcgg
     2521 cctccccgtc aaggggtccg cgacccgtga agcacgcggc gacgatccgc tcccagcgct
     2581 ccgtgaggac ctcgtccatc tcgcgggacg cctcatctcg gaggtccgct cgcccttcgc
     2641 gcagagcggt agagacgacc caggagaggc cgaagagatg tgctgcgcga gggttcgatg
     2701 tgagcttcct tttcagccgg tccgcgccgg ccctcacgcg gtcgcccggt gccgccttgc
     2761 ccgcggcgag gaatgcggag tgctcgatga gcgcggcgta ggtctcccag gtgccctccg
     2821 agggtgcccg caggcgcttc gcgtcccgac gagagacggc ctccgcgagc gccacgccga
     2881 ggagctcctc cgcagagcgg gccgggtcga cgctgcgcag ggcgccggcc aggtagtaga
     2941 gcggcaggga gagagagagc aggagcccga gcggctcgtc gacgtcgcgt tccgcgatga
     3001 gtgcgcgcgc cctctttccc gcctcgattc tcgcccccca agccgcctcc acgatgtgcg
     3061 ttcccgggtg aaccgtcttc gccacgtctc gctccgttcg ctcctcgaga gaggtccggg
     3121 ccggcgggac gcgggagcga agtcgcttcg gcgcggtgat cagccacgcc gcccgccggc
     3181 ccggaggaat gatcaggctt ccttcatctc ctgcccgaac cgatcgagcc gcttccgccg
     3241 gcccttcgcc cgggcgaagt cctcggccgt ccactcctcg acgacgcgcc ccgtcggccg
     3301 gtgaacggtg atcgctacgg cgacgatgcc cgcggccccg ttgccgccga cgtgagcctg
     3361 gtacgtgtcg cccgggttca ccttgatgct gctcatctcg ctcctttccg atggcgccgt
     3421 caatggcgcc acggtgccgg aaaggtgcgt tctatgcggg tcgcgtccga gtcgctccgt
     3481 cgcgaagtcc gctagatacg cgcctttcac tactcacgga gtgagtctgt gctcaacgtc
     3541 tgacggggct ccggcgccgt cgccggaccc ggaggcgcct cagcccttcg gctcggagcc
     3601 gggctcccct gcggcctcgc cgccggcggg ggccggaggc tcctcgtcct tcgcggggag
     3661 gccggccgga ggggtctccg gcggggactc gacgacggga accgggatct cgtcggtggg
     3721 agtgaggtct tcgtcgtcga ccttcgcgac ccggagcgag accgaggtct tctcccggga
     3781 cgtgtcggcc tttctcgcct cgccgttccg cacctcgacc gtgatggcgg tgatgttgtc
     3841 cacgccgccg cgctcgttgg cgaggtcgat cagcccgcgc acgatctgct ccagcgagcg
     3901 cgccgaggag agagcctcgg tgatctcctc gtccgagacc atccccgtca ggccgtcgga
     3961 gcagaggagg aagcggtcgc cgtcgccgac cgggatctcg atgaggtcga cggcgacgtg
     4021 cgctcccccg ccgagggcgc gggtgacgac gttcttgagc gggtggctcc gcgcctcctc
     4081 ctcggagagg atgcccgcgt tgacctgctc ctgcacccag gagtggtcgt cggtcaggcg
     4141 gcgcagctcc gagtcgcggt agaggtacgc ccggctgtcg ccgacgtgga cgagcgtggc
     4201 ccgcttctcg tcgaacagcg cggcgacgac ggtcgtcccc atccccttca gctcggggcg
     4261 gttcacgacg gcgcgcatga ccttctcgtt cgcgcgctcc accgcggtgg agaggcggtt
     4321 cccgtcgacg gagtagcggt tgttgtaccc gaacggccac gtgttgtcgt ggtcgtcctc
     4381 ggtaccggcg atgaattcct ggatcgactc gaccgtcagg cgcgaggcga cctcgcccgc
     4441 ggcgtgcccc cccatgccgt cggcgacgac gaagaggccg cgctcggcgt cgagcaggta
     4501 ggcgtcctcg ttgtgcttcc tcttccggcc gacgtcggtg agaccg
//
LOCUS       DAOONL010000211         4507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23212_length_4507_cov_20.156110, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000211 DAOONL010000000
VERSION     DAOONL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23212_length_4507_cov_20.156110"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLP50_16835"
     CDS             <1..1272
                     /locus_tag="PLP50_16835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA53260.1"
                     /translation="RESIHAHSIVSFLCVPLLVKERASGAIYVDHRNAPRLFSEGDRA
                     FLEAFADLAAVAIENARLVEELLEARLRLSVENESLKANLGRGWNLDALVGSSESASR
                     IKATLPRAAAGPVTVLIRGESGTGKNLVARILHALSPRAQGPFIQFNCAALPETLAES
                     ELFGHEKGSFTGADRKKPGRFELAQGGTIFLDEIGKTSLGIQAKLLRVVEDKEFERVG
                     GTQTLKCDAKIVAATNLDLEAAIRANEFREDLFYRLNVVPIELPPLRSRLEDLPALVE
                     HFVVKLSRDFGADPRRVEPEVLELFARYPWPGNIRELEATLHRALVLCPKERLGPEDF
                     SWILDSPLLLAGRPLVPRPPAPFGHEPPVDPDYFEARMSGLERELIERALAEAGGRIR
                     EATRRLGLARNTLKAKMAKYGLRGQEEGSAP"
     gene            1362..2522
                     /gene="tal"
                     /locus_tag="PLP50_16840"
     CDS             1362..2522
                     /gene="tal"
                     /locus_tag="PLP50_16840"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPA53261.1"
                     /translation="MNRSVDYRKRGQAVWLDSISARMIATGELAGRIAAGSVYGVTSN
                     PTIFGQAIGKGDGDYEDRIANLADEGKDAFGVYDELTRGDIAAGADLFAAQHAANAED
                     GWISLEVLPSLAFDIDGTIAEAKRLHRLLGRPNVFIKVPATPAGCVAIRQLIASGVSV
                     NVTLMFNRKHYRDVAHAYQAGIEALVAAGGDPAKVSSVASFFVSRVDSLVDKLLGERA
                     AIASGALKQKLEGLMGKAALANAKVVYQDYLAAFGTDAWRALAAKGARVQRPLWASTG
                     TKNPAYSDLLYVENLVGPDTVNTLPEKTLLALLDHGRDLGDTVLEGVDEARRTLGELA
                     DLGIDVEALGEKLQGEGVVLFERSYDELLAVVEERRRAALRESAQTVRIRPK"
     gene            2533..4038
                     /gene="zwf"
                     /locus_tag="PLP50_16845"
     CDS             2533..4038
                     /gene="zwf"
                     /locus_tag="PLP50_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPA53262.1"
                     /translation="MSPAPAPAATIVIFGGSGDLARRKLVPALFELHRQGLLAPETAI
                     VGFARSVGSDEEWRDELAAALDEHGRGGEDAEEARRSFASRLHAVRGDLRSDASYAEM
                     KRRLEGIEAARGLPGNRLYYLAIPPSGIGDAARRLGAAGLVHPVEGDGPFSRLVVEKP
                     FGHDLASAHALNEAVHASFRERQLFRIDHYLGKETTQNILVFRLGNGIFEPLWNRRYV
                     DHVQITVAETIGVEGRGKFFEEAGIFRDVVQNHLLQLLCLVAMEPPVAFEANVVRDEK
                     VKVLRSVRPPSREEVLRDTVRGQYAAGAISGRAVAGYRDEPDVAAASTRETYAAWKLQ
                     IENWRWAGVPFYLRAGKRLPKRVTEIAITFKTPPIALFRHAGCAVGEPNVLVLRIQPD
                     EGIALRIGAKRPGPTMRVETVKMDFRYVEQFGEATPDAYERLLLDALNGDGTLFARRD
                     EVEAAWELVEGVLDVWAESPPVDLPNYEAGTWGPEAATELLARDGRRWRRP"
     gene            4053..>4507
                     /locus_tag="PLP50_16850"
     CDS             4053..>4507
                     /locus_tag="PLP50_16850"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPA53263.1"
                     /translation="MATTQVTVFPSAAALAEGVAELFAVRAAEAVAERGRFRAALAGG
                     TTPRAAYRRLADAPWAELVPWGSVELFFGDERAVGECDGERNDAAARDALLLRVPIPP
                     ENVHPVPALAPDGAERYEALLRARLGAPAPAIPVLDLVLLGLGEDGHTAS"
BASE COUNT          635 a         1689 c         1585 g          598 t
ORIGIN      
        1 cgcgagtcga tccacgcgca ctcgatcgtc tcgttcctct gcgtcccgct cctcgtgaag
       61 gagcgtgcct cgggggcgat ctacgtcgac caccgcaacg ccccccgcct cttctccgag
      121 ggggaccgcg ccttcctcga ggcgttcgcc gacctcgcgg cggtcgccat cgagaacgcg
      181 cgcctcgtcg aggagctgct cgaggcgcgc ctccgtctct ccgtcgagaa cgagagcctg
      241 aaggcgaacc tcgggcgcgg ctggaacctc gacgcgctcg tcggcagctc ggagtcggcg
      301 agccggatca aggcaaccct cccgcgcgcg gcggccgggc cggtcacggt cctgatccgc
      361 ggggagtcgg ggaccggcaa gaacctcgtc gcgcggatcc tccacgccct ctcgccgcgc
      421 gcgcaggggc cgttcatcca gttcaactgc gcggcgctcc cggagacgct cgccgagtcg
      481 gagctgttcg ggcacgagaa gggctccttc accggcgccg accgcaagaa gcccgggcgc
      541 ttcgagctcg cccagggggg gacgatcttc ctcgacgaga tcgggaagac gtcgctcggc
      601 atccaggcca agctcctgcg cgtcgtcgag gacaaggagt tcgagagggt cggcgggacg
      661 cagacgctga agtgcgacgc gaagatcgtc gcggcgacga acctcgacct cgaggcggcg
      721 atccgggcga acgagttccg ggaggacctc ttctaccggc tgaacgtcgt gccgatcgag
      781 ctcccgccgc tccggagccg gctggaggac ctgccggcgc tcgtcgagca cttcgtcgtg
      841 aagctctcgc gcgacttcgg cgccgacccc cgccgcgtcg agccggaggt cctcgagctc
      901 tttgcccgct acccgtggcc ggggaacatc cgcgagctgg aggcgacgct ccaccgcgcg
      961 ctcgtcctct gccccaagga gcggctcggg ccggaggact tctcgtggat cctcgactcc
     1021 ccgctcctcc tcgcggggcg tccgctcgtc ccgcgccccc cggccccctt cggccacgag
     1081 ccgccggtcg accccgacta cttcgaggcc cggatgtccg gcctcgagcg cgagctgatc
     1141 gagcgcgccc tcgccgaggc gggcggacgg atccgcgaag ccacgcgccg cctcggcctc
     1201 gcgcggaaca cgctcaaggc gaagatggcg aagtacggcc tcaggggaca ggaggaaggg
     1261 tccgcgccgt agcgggccgc ggcgctcgcc gccgcaacgc cggtatacga tgcgaggacc
     1321 ctcgggacgg cccggccgtc cgacatccca ggaggacccg aatgaaccgc tccgtcgact
     1381 accggaagcg tggccaggcc gtctggctcg acagcatctc ggcccggatg atcgccacgg
     1441 gagagctcgc gggccgcatc gcggccggct ccgtctacgg cgtcacctcg aacccgacga
     1501 tcttcggcca ggcgatcggc aagggagacg gcgactacga ggaccggatc gcgaatctcg
     1561 ccgacgaggg gaaggacgcc ttcggcgtct atgacgagct gacccgcggc gacatcgccg
     1621 ccggtgccga tctcttcgcc gcgcagcacg ccgcgaacgc cgaggacgga tggatctcgc
     1681 tcgaggtcct gcccagcctc gcgttcgaca tcgacgggac gatcgcggag gcgaaacgcc
     1741 tccaccgcct cctcgggcgc ccgaacgtct tcatcaaggt cccggcgacg cccgccggct
     1801 gcgtcgccat ccgccagctg atcgcctcgg gcgtctccgt gaacgtgacg ctcatgttca
     1861 accggaagca ctaccgggac gtcgcgcacg cctaccaggc ggggatcgag gcgctcgtcg
     1921 ccgccggcgg cgacccggcg aaggtctcct ccgtcgcgtc gttcttcgtc tcgcgcgtcg
     1981 actcgctcgt cgacaagctc ctcggcgaga gggcggcgat cgcctccggg gcgctgaagc
     2041 agaagctcga ggggctgatg gggaaggccg cgctcgcgaa cgcgaaggtc gtctaccagg
     2101 actacctcgc cgctttcggg accgacgcct ggagggcgct cgcggcgaag ggggcgcgcg
     2161 tccagcggcc gctctgggcc tccaccggga cgaagaaccc ggcctactcc gacctcctgt
     2221 acgtcgagaa cctcgtcggc cccgacaccg tgaacacgct ccccgagaag accctcctcg
     2281 cgctcctcga ccacggccgc gacctcggcg acaccgtcct cgaaggggtc gacgaggcgc
     2341 gccggacgct cggcgagctg gccgatctcg ggatcgacgt cgaggccctc ggcgagaagc
     2401 tccagggaga gggggtcgtc ctcttcgagc gttcgtacga cgagctcctc gccgtcgtcg
     2461 aggagaggcg gcgggccgcc ctccgcgaat cggcccagac ggtgaggatc cggccgaagt
     2521 gacggaaggc gcgtgagccc cgccccggcc cccgcggcga cgatcgtcat cttcggaggc
     2581 tcgggcgacc tcgcgcgccg aaagctcgtt ccggccctct tcgagctcca tcgccagggg
     2641 ctcctcgcgc cggagacggc gatcgtcggc ttcgcccgct cggtcggctc cgacgaggag
     2701 tggcgcgacg agctcgccgc cgccctcgac gagcacgggc gtggaggcga ggacgcggag
     2761 gaggcgcggc ggtccttcgc ctcccggctc cacgccgtcc ggggagacct ccggagcgac
     2821 gcgagctacg cggagatgaa gcgccgtctc gaggggatcg aggcggcgcg cggcctgccg
     2881 ggcaaccggc tctactacct cgccattccc ccgtcgggga tcggcgatgc cgcccggagg
     2941 ctcggcgcgg ccgggctcgt tcacccggtc gaaggggacg gccccttctc gcgcctcgtc
     3001 gtcgagaagc cgttcggcca cgacctcgcc tccgcgcacg cgctcaacga ggcggtccac
     3061 gcctccttcc gcgagcggca gctcttccgg atcgaccact acctcggcaa ggagacgacg
     3121 cagaacatcc tcgtcttccg cctcggcaac ggcatcttcg agccgctctg gaaccgccgc
     3181 tacgtcgacc acgtccagat cacggtcgcc gagacgatcg gcgtcgaggg gcgcgggaaa
     3241 ttcttcgagg aggcggggat cttccgcgac gtcgtccaga accacctcct ccagctcctc
     3301 tgcctcgtgg cgatggagcc gcccgtcgcg ttcgaggcga acgtcgtccg cgacgagaag
     3361 gtcaaggtcc ttcgctcggt ccggccgccg tcgcgggagg aggtcctccg cgacacggtc
     3421 cgcgggcagt acgccgcggg cgcgatctcg ggacgggccg tcgcgggcta ccgcgacgag
     3481 cccgacgtcg cggccgcgtc gacgcgggag acctacgccg cctggaagct ccagatcgag
     3541 aactggcgct gggccggcgt cccgttctac ctgcgggccg gcaagcggct gccgaagcgg
     3601 gtcaccgaga tcgcgatcac gttcaagacg ccgccgatcg ccctcttccg gcacgcgggc
     3661 tgcgccgtcg gcgagccaaa cgtcctcgtc ctccggatcc agcccgacga ggggatcgcg
     3721 ctccggatcg gcgccaagcg ccccgggccg acgatgcgcg tcgagaccgt gaagatggac
     3781 ttccgctacg tggagcagtt cggcgaggcg acgcccgacg cctacgagcg gctcctgctc
     3841 gacgccctga acggcgacgg caccctcttc gcgcggcgcg acgaggtgga agcggcgtgg
     3901 gagctcgtcg agggggtcct cgacgtctgg gccgaaagcc cgccggtcga cctgccgaac
     3961 tacgaggcgg gaacgtgggg ccccgaggcg gcgaccgagc tcctggcgcg cgacggccgg
     4021 cgctggcggc ggccgtagga ggggccggcc ccgtggcgac gacgcaggtc accgtcttcc
     4081 cgtcggccgc ggcgctcgcg gagggggtcg cggagctttt cgccgtccgc gcggccgagg
     4141 cggtcgccga gcgcggacgc ttccgcgcgg cgctcgccgg agggacgacg ccgcgcgccg
     4201 cctaccgccg cctcgcggac gcgccctggg ccgagctcgt cccctggggc tccgtcgagc
     4261 tcttcttcgg cgacgagcgc gccgtcggcg agtgtgacgg ggagcggaac gacgccgccg
     4321 cgcgcgacgc gctcctcctg cgcgtcccga tcccgcccga gaacgttcac cccgtcccgg
     4381 ccctcgcgcc ggacggcgcg gaacgctacg aggcgctcct tcgcgcgcgg ctcggcgcgc
     4441 ctgcgcccgc gatcccggtc ctcgacctcg tcctcctcgg cctcggcgag gacggccaca
     4501 cggcctc
//
LOCUS       DAOONL010000212         4502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23244_length_4502_cov_12.930065, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000212 DAOONL010000000
VERSION     DAOONL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23244_length_4502_cov_12.930065"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLP50_16855"
     CDS             complement(<1..491)
                     /locus_tag="PLP50_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPA53264.1"
                     /translation="MPSAIRIVKDDPTASLVDFFENRSVGAALRQVVAPRNVRQQEYL
                     QAIGERDVVFAIGPAGTGKTYLAVAMAAAALLEKRVKRIVLCRPAVEAGEKLGFLPGD
                     LAEKVNPYLRPLYDALYDILGYEKVGRLLERQVIEIAPLAFMRGRTLNDAFIILDEAQ
                     NTTS"
     gene            800..2317
                     /locus_tag="PLP50_16860"
     CDS             800..2317
                     /locus_tag="PLP50_16860"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPA53265.1"
                     /translation="MIRLYTVVFLSGAALMGLEIAGSRVMAPVFGTSIFVWGSLITTF
                     LASLAAGYALGGRLADRRPRASLLGGILVGAGLLVWLPLARPAPLLALCAAAPVPDRF
                     QALLAALLLFALPSVLMGAVSPFAMRLAAREVGTIGRTAGSLAALSTAGSIVGTFSMT
                     FLFIPAFPIEPILFGVGATLVLCGAFASRDGLPGRLGFAAAGLAAAAAVFLVRPERVP
                     SPVPDGTVVFRKETAYHRLLVVDQGPRRVLFFNNLAQGFVSRSSGMATGRVYSDGLAA
                     SLLFRKAPARNAFVIGLGTGMLPRLLSERAPEIATTSVEIDPEVVRVAGEYFGFRPDA
                     NDRVLVGDGRTVLERERGPWDAIILDAYFSDSLPFHLTTREFLELCRERLSPDGVFSA
                     NFVGTLMGKEQRLFWSFVRTAREVFPTVAILSAELSSGKPVFQSNAILVASPSRDRLS
                     KERVVAEGDRLAAALHHPPIAEWALEYHEGELRTDEVPVLTDSFSPTDALQHLGR"
     gene            complement(2416..>4502)
                     /locus_tag="PLP50_16865"
     CDS             complement(2416..>4502)
                     /locus_tag="PLP50_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPA53266.1"
                     /translation="GQSFLDFTITDADSGTTLVRTGTAWEATAAQRLKFTASGTTGDV
                     AWNFGDGTTSAEASPIKVYSPAVDTPYTVTLTNGANQKQYTINVKGSTGAPLTGNYSY
                     RYVDGTNVNRLAVDPNKPIRFTGADQATTYTWDFGDGSPLAQGSPKDYSFTRGGTFTV
                     KLTVARNGVPGTVTTASPTVFTVKPPPDPLLWVAGGMAYTDNGAGVKWQSDLSIHNPG
                     TTTATVSIAYVAGATWDGVSKVTWIPQALVAGETKSYTNVLKSLFLLDQGAWGVVLVR
                     GDDIPVPPVIVSRTYNAANADEVGTFGLSVPALSVAGGVQPQSAGGGNVLAGLRHDDV
                     FRTNLAVANLKGETAEVEVVFRDVNGNALGVPALITVEARGVKQIGSVLSADPAAGGA
                     AIGGAGYRTPISDFSAEVKLRKGSGVYPYATVIDNGTGDSIVVTPAARPSPRLRLPGV
                     VRVQGKSGPFWMSDVAVLNPSDKERRVRITYSYRKVGAAGRATMENTLVFKPWEMKVA
                     VDFVKTWLGLAESDLAGYADSFVDISPAADDPAPTENLVVVGKTYMATEDGSIGLQLD
                     GFAYEDGISAQGSRRRVVLSGLEANRDYRTNLALFLTPGSTGSVQVDVRVLDSFGRES
                     KKISSVGLNADAPFVQIGNDSLFAGLTTGEMSRATIVIDNPRGTAVVGAYATVIDNRS
                     EDATFVAGQPAP"
BASE COUNT          588 a         1600 c         1557 g          757 t
ORIGIN      
        1 gaggtcgtgt tctgcgcctc gtcgaggatg atgaacgcgt cgttcagcgt ccggccgcgc
       61 atgaaggcga ggggtgcgat ctcgatcacc tgccgctcca ggagacgccc gaccttctcg
      121 tagcccagga tgtcgtagag ggcgtcgtag agcgggcgaa ggtaggggtt caccttctcg
      181 gcgaggtcgc ccgggaggaa gccgagcttc tctcccgcct cgaccgccgg gcgacagagg
      241 acgatccgct tgacccgttt ctcgaggagc gccgcggccg ccatcgcgac ggcgaggtag
      301 gtcttcccgg tgccggcggg cccgatcgcg aagacgacgt cccgctcgcc gatcgcctgg
      361 aggtactcct gttggcggac gttgcgcggg gcgaccacct gccgcagggc ggcgccgacg
      421 gagcggttct cgaagaagtc gacgagtgag gccgtggggt cgtccttgac gatccgaatc
      481 gcgctcggca cgtcggactt cttcggcacg tagccccgcc cgatcagctt cgagaactcg
      541 gagaggaggt gcgccgcccg ctccgtccct tccggtcccc cgctgatcga gacgaccccg
      601 ccccgcgccg agatctcgac gccgaaggcg tcctccagga gcctcaggtt ttcgtcgtgg
      661 acaccgaaga gcgcgctcag cccggattcg ggcaggttca gggggatcgg tccgtattgg
      721 atagcgctgt ctcctcgtcg aaagcatagc ggcgggcgcg agggccggtc aaatgacctc
      781 tccgccctag aatccggggg tgatccgcct ctacaccgtc gtctttctct ccggcgccgc
      841 gctgatgggc ctcgagatcg ccgggagccg cgtgatggcc ccggtcttcg gcacctcgat
      901 cttcgtctgg ggctcgctca tcacgacgtt cctggcgtcg ctcgccgctg gctacgctct
      961 cggcggccgc ctcgccgacc gccgcccgcg cgcgtcgctc ctcggcggga tcctcgtcgg
     1021 cgccggcctc ctcgtctggc tccccctcgc ccgccccgcg ccgctcctgg ccctctgcgc
     1081 ggccgcgccg gtccccgacc ggttccaggc gctcctcgcc gcgctccttc tcttcgcgct
     1141 tccgagcgtc ctgatggggg ccgtctcccc gttcgcgatg cggctggccg cccgcgaggt
     1201 cggcacgatc ggccggacgg ccggctccct cgccgccctc tcgacggccg ggtcgatcgt
     1261 cggcaccttc tcgatgacgt tcctcttcat ccccgccttc ccgatcgagc cgatcctctt
     1321 cggcgtcggg gcgacgctcg tcctctgcgg cgctttcgcc tcccgggacg gactccccgg
     1381 gcggctcggc ttcgcggccg ccgggctcgc cgccgcggcc gcggtcttcc tggtccgacc
     1441 ggagcgcgtc ccgtcgcccg ttccggacgg gacggtggtc ttccggaagg agaccgccta
     1501 ccaccggctc ctcgtcgtcg accagggccc ccgccgagtc ctcttcttca acaacctcgc
     1561 ccaggggttc gtgagccgca gctccgggat ggcgaccggg cgggtctact cggacggtct
     1621 cgccgcctcg ctcctcttcc ggaaggcgcc ggcgcggaac gccttcgtca tcggcctcgg
     1681 cacggggatg ctcccccgcc ttctctccga aagggctccc gagatcgcca cgacctcggt
     1741 cgagatcgac ccggaggtcg tccgcgtcgc cggcgagtat ttcggctttc gccctgacgc
     1801 caacgaccgc gttctcgtcg gcgacggccg cacggtgctc gagcgggaga gagggccatg
     1861 ggacgcgatc atcctcgacg cctacttctc cgacagcctc ccgttccacc tgacgacccg
     1921 ggagttcctc gagctctgcc gggaacgcct ctcgccggac ggcgtcttct ccgcgaactt
     1981 cgtcgggact ctcatgggca aggagcagcg gctcttctgg tcgttcgtcc ggaccgcccg
     2041 ggaggtcttc ccgacggtcg cgatcctctc cgccgagctc tcgtcgggca aaccggtgtt
     2101 ccagagcaac gcgatcctcg tcgcttcccc ttcgcgcgac cgtctctcga aggagcgcgt
     2161 cgtggccgag ggggaccgcc tcgcggccgc cctgcaccat cctccgatcg ccgaatgggc
     2221 cctcgagtac cacgagggcg agctccgaac cgacgaggtc ccggtcctca ccgactcgtt
     2281 cagcccgacc gacgctctcc agcacctcgg acgctgaggc cgggcgcgaa aaaaagcggc
     2341 ccaggcggga gcccgggccg atgtgagggg tgagcccgac ggtttcgggc ttgggggtct
     2401 cgtttcaggc cggcatcagg gcgccggctg gcccgccacg aacgtcgcgt cctcggaccg
     2461 gttgtcgatc acggtcgcat acgcgccgac gaccgccgtt ccgcgggggt tgtcgatcac
     2521 gatcgtggcc cgcgacatct cgccggtcgt caggcccgcg aagaggctgt cgttgccgat
     2581 ctgcacgaac ggcgcgtcgg cgttcaggcc gacggaggag atcttcttcg actcgcggcc
     2641 gaacgagtcg aggaccctca cgtcgacctg aacgctcccg gtcgagccgg gcgtcaggaa
     2701 gagagcgagg ttcgttcgat agtcccggtt cgcctcgaga ccggaaagga cgactcgccg
     2761 gcgggagccc tgcgcgctga tcccgtcctc gtatgcgaag ccgtcgagct gaaggccgat
     2821 cgacccgtcc tccgtcgcca tgtaggtctt gccgacgacg acgaggttct ccgtcggggc
     2881 ggggtcgtcc gcggccggcg agatgtcgac gaaggaatcg gcgtagccgg ccaggtcgct
     2941 ctcggcgagg ccgagccagg tcttcacgaa gtccacggcg accttcatct cccagggctt
     3001 gaagaccagc gtgttctcca tcgtggcgcg cccggcggca ccgaccttcc tgtacgaata
     3061 ggtgatccgg acccggcgtt ccttgtcgct cgggttgagg acggccacgt cgctcatcca
     3121 gaacggcccg ctcttcccct ggacccggac gacgcccggg agccggaggc ggggcgacgg
     3181 acgcgcggcc ggcgtgacga cgatcgagtc gcccgtgccg ttgtcgatga cggtggcgta
     3241 cgggtagacg ccggagccct tgcggagctt cacctcggcc gagaagtccg aaatcggagt
     3301 gcggtacccc gcgccaccga tcgccgcgcc gcccgcggcc ggatcggccg agaggaccga
     3361 gccgatctgc ttgacgcctc gcgcctcgac cgtgatcagg gccggcacgc cgagcgcgtt
     3421 tccgttgacg tcgcggaaga cgacctccac ctccgcggtc tcgcccttca ggttcgcgac
     3481 cgcgaggttc gtgcggaaga cgtcgtcgtg ccgaaggccg gcgaggacgt tcccgccgcc
     3541 ggcggactgg ggctggacgc ccccggcgac cgagagcgcc gggacggaga ggccgaacgt
     3601 gccgacctcg tcggcgttcg cggcgttgta cgtccggctc acgatgacgg gcggaaccgg
     3661 gatgtcgtct ccgcggacga ggacgacgcc ccaggcgccc tggtcgagca ggaagaggct
     3721 cttcaggacg ttcgtgtacg acttcgtctc gccggcgacg agcgcctgcg ggatccaggt
     3781 gaccttggag acgccgtccc aggtggcgcc ggcgacgtag gcgatggaaa cggtggccgt
     3841 cgtcgtcccg gggttgtgga tcgagaggtc ggactgccac ttcacgcccg cgccgttgtc
     3901 cgtgtaggcc atcccgcccg cgacccagag gagcgggtcg ggcggtggct tgacggtgaa
     3961 gacggtcggc gaggcggtcg tgaccgtgcc gggcacgccg ttgcgcgcca cggtcagctt
     4021 cacggtgaac gtgccacccc gcgtgaacga gtagtccttc ggggagccct gcgcgagagg
     4081 cgagccgtcg ccgaagtccc aggtgtaggt cgtcgcctgg tcggcgccgg tgaagcggat
     4141 cggcttgttc gggtcgacgg cgagacggtt cacgttcgtc ccgtcgacgt accggtagga
     4201 gtagttcccc gtgagaggcg cgccggtcga gcccttcacg ttgatcgtgt actgcttctg
     4261 gttcgccccg ttcgtgaggg tgacggtata gggcgtgtcg acggccgggc tgtagacctt
     4321 gatcgggctc gcctccgcgg aggtggtccc gtcgccgaag ttccacgcga cgtcgccggt
     4381 ggtgccgctc gcggtgaact tcaggcgctg ggccgccgtc gcttcccagg ccgtccctgt
     4441 gcggacgaga gtcgtcccgc tgtcggcgtc cgtgatcgtg aagtcgagga agctctggcc
     4501 gc
//
LOCUS       DAOONL010000213         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23321_length_4489_cov_18.617050, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000213 DAOONL010000000
VERSION     DAOONL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23321_length_4489_cov_18.617050"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1098)
                     /locus_tag="PLP50_16870"
     CDS             complement(109..1098)
                     /locus_tag="PLP50_16870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA53267.1"
                     /translation="MEQAVAIPPTDFRPELLDRRERLLAIARVRPPDKGLSHLLAEVD
                     AALGRIEAGTFGFCETCHDPIEPEYLAREPIARFCIGDLSPAEQRTLEEDLETAARIQ
                     KAHLPQVATELAGWEFAFAFEPAGPVGGDCCDVLGHPGGEATFLLGDVSGKGVAAAML
                     AASLHATIRTLASPGTPVEALVERANRLFRSGSVSRAFATLVCGRVSPDGTLAVCNAG
                     HCPPLLSTGGRIREIDPTGLPIGTFLSTAYGGHALRLSKGDFLVAFTDGLTEARDASG
                     AEYGSERLAATLSGLDRPTARGVVDTCLADLHSFTGGRAPEDDLSILAIRRSA"
     gene            complement(1225..4437)
                     /locus_tag="PLP50_16875"
     CDS             complement(1225..4437)
                     /locus_tag="PLP50_16875"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53268.1"
                     /translation="MSGSDEENQPGIYGTKGIAAPANVPGARKEAVSWIDANGGFWLF
                     GGQGYTQDGLGQLNDLWRWDGTSWTWIAGSDTLDQPGIYGTKGIPSPSNVPGARERSV
                     SWTDASGNLWLFGGYRSAQEGGERLNDLWKWDGTSWTWISGSDAVNQPGAYGTKGTAA
                     PGNAPGARSSAITWTNAAGEVWLFGGFGYGATGVGFLNDLWRWDGTNWTWMSGSDAVN
                     QPGTYGTKGIAASGNVPGSRSGAASWMDTDGSLWLLGGYDTYPSWTGMFDDLWDLGSR
                     NDLWRWDGTSWTWVSGSDDSHAHSEYGTQDVPAPGNTPGARSNTITWTDATGGLWLFG
                     GVGMRWGSGGRPLGDLWKWDGTNWTWSSGDGESASGTYGTKGVASPENHPGWRSSSVS
                     WTDGGGGRWLFGGSTVDDYSIQGFLNDLWRWDGTNWTWMSGSNVPNQLGTYGTKGIAA
                     PTNVPGARYGAVSWTDAGGNLWLFGGVFEEYWVWETSFYRFNDLWKWDGTSWTWMSGS
                     NVSDQPGTYGTKGLAAPGNVPGAREGAVSWTDAAGDFWLFGGYGYGATGVGDLNDLWK
                     WDGTNWTWMSGSDAVNQPGTYGTKGLAAPSNVPGARERAVSWTDGAGNFWLFGGYGYG
                     ATSSGELNDLWRWDGTSWTWMSGSSSPNQPGVYGTRGVAAAENTPGGRYWAVSWTDAI
                     GNLWLFGGSGFAATADGYLNDLWKWNGTSWTWVAGSNTPNQPGTSGSQSVAAPANTPG
                     ARGGAVSWIDASGNPMLFGGYGYDAIGLEGDLRDIWVFGAQCAFLDPPTVGNGGPYAV
                     GATVALTASSVPGAAYLWTGPNGFTSSEQNPTIPNATTETAGLYSVTASAGACTSPAA
                     TTTVVVLDAQTLAVSRIGSGSGSVTSAPEGIACGADCVASFPGLSQVTLTATPDAGSR
                     FAGWAGGGCFGTEPCTVTMNGAKSVSATFLSSAGVGFHTVTPCRVVDTREATGPFGGP
                     ALSAGVARAFAIAGTCGVPADASAVALNVTVANPTSAGSLTLYPGTGIVPGATTISFT
                     AGRTRANNATMALIDGFLSVLDDQETGTTDVIIDVSGYYR"
BASE COUNT          645 a         1597 c         1516 g          731 t
ORIGIN      
        1 cgcatcggcg ttccttcctt tcgaacggtt cggcgaacga aacgaaggcc gtgtgcgatc
       61 ggttcccgcg atcggcccgc tttctcttcg gacggcgcgc gctggccctc aggccgaccg
      121 gcggatggcg aggatcgaca ggtcgtcctc cggcgcgcgg cctccggtga aggagtggag
      181 gtcggcgagg caggtgtcga cgacgcctcg cgccgtcggt cggtcgagcc cggaaagggt
      241 cgcggcgaga cgctcggagc cgtattcggc gccgctcgcg tcgcgagcct ccgtcagccc
      301 gtcggtgaag gcgacgagga aatctccctt ggagagccga agggcgtggc cgccgtacgc
      361 ggtgctcagg aacgtgccga tggggaggcc cgtcggatcg atctcccgga tccggccgcc
      421 cgtggagagg agcggggggc agtggcccgc gttgcagact gcgagggtcc cgtcggggga
      481 gacccggccg cagacgagcg tcgcgaaggc gcgggaaaca gagccgctcc ggaagaggcg
      541 gttcgcccgc tcgacgagag cctcgaccgg ggttccggga gaggcgagcg tccggatcgt
      601 ggcgtggagg ctcgcggcga gcatggcggc ggcgacgccc ttgccggaga cgtcgccgag
      661 gaggaaggtc gcctctcctc cggggtggcc gaggacgtcg cagcagtctc cgccgaccgg
      721 gcccgccggc tcgaaggcga acgcgaactc ccacccggcg agctccgtgg cgacctgcgg
      781 gaggtgcgcc ttctggatac gggcggccgt ctcgaggtcc tcctcgaggg tccgctgctc
      841 cgcaggggag aggtcgccga tgcagaaccg ggcgatcggc tcccgggcga ggtattccgg
      901 ctcgatcggg tcgtggcacg tctcgcagaa gccgaacgtc ccggcctcga tccggccgag
      961 cgcggcatcg acctcggcga ggaggtgcga caaccctttg tccggtggcc ggacgcgggc
     1021 gatcgcgagc agcctctccc ggcggtcgag gagctcgggg cggaagtcgg tcggagggat
     1081 cgcgacggcc tgttccatgt gtgtctcccg ggtcaggtcg ttctacgtaa cctgccggct
     1141 tcggtttcgg acggcatcgc ctgaccgtga gaatcggctg cggaatccct cacggagagt
     1201 cgtggctcgg cgtcgtcgcc gggttcagcg gtaatacccg ctcacgtcga tgatgacgtc
     1261 ggtagtgccg gtctcttgat cgtcgaggac agacaggaag ccgtcgatca gggccatcgt
     1321 ggcgttgttc gcgcgagtcc tgccggcggt gaacgagatc gtggtcgctc cgggcacgat
     1381 ccccgttccg ggatagagcg tgaggctgcc ggcggaggtc gggttcgcga cggtgacgtt
     1441 cagcgccacg gcggaagcgt cggcagggac gccgcacgtg cctgcgatcg cgaaggcgcg
     1501 tgccacgccc gcggagaggg cgggaccgcc gaacggaccc gtcgcctcgc gcgtgtcgac
     1561 gacgcggcac ggggtgacgg tgtggaagcc cacccccgcg gacgacagga acgtcgccga
     1621 gacggacttg gccccgttca tcgtcacggt gcacggctcg gtgccgaagc accctcctcc
     1681 ggcccagccg gcgaagcgcg agccggcgtc gggagtcgcc gtgagcgtga cctgcgacag
     1741 gcccgggaac gaggcgacgc agtcggcccc gcaggcgatt ccttcgggag ccgaggtgac
     1801 gctcccgctg cctgagccga tcctcgagac ggcgagggtc tgtgcgtcga ggaccacgac
     1861 ggtcgtcgtc gccgcgggcg aggtgcaggc gccggcggag gccgtgaccg agtagaggcc
     1921 ggccgtctcc gtcgtcgcgt tcggaatcgt cgggttctgt tccgacgagg tgaagccgtt
     1981 cgggcccgtc cagagatagg cggcaccggg gacggacgag gccgtgagcg cgaccgtcgc
     2041 gccgacagcg tagggtccgc cgttgccgac cgtcggaggg tcgaggaagg cgcactgcgc
     2101 cccgaagacc cagatgtcgc gcaggtcgcc ctcgagcccg atcgcgtcat acccgtagcc
     2161 cccgaagagc atggggttcc cgctcgcgtc gatccacgac acggcgccac cccgcgcccc
     2221 gggcgtgttc gcgggtgcag cgacgctctg agatccggag gtccccggct ggttcggcgt
     2281 gttcgagccg gccacccacg tccagctcgt tccgttccac ttccagaggt cgttcaggta
     2341 gccgtccgcc gtcgccgcga atcccgagcc cccgaagagc cagaggttcc ctatggcgtc
     2401 cgtccacgaa acggcccagt accggccgcc cggcgtgttc tcggccgcgg cgacacctct
     2461 cgtcccgtag accccgggct ggttcggaga gctcgatccg ctcatccagg tccagctcgt
     2521 cccgtcccat cgccagaggt cattgagctc tcccgaagac gttgccccgt acccgtagcc
     2581 accgaacagc cagaagttcc cggccccgtc cgtccaggag acggctcgct ctcgcgctcc
     2641 cggcacgttg ctgggcgccg cgagcccctt cgtcccgtac gtcccgggct ggttcacggc
     2701 gtccgagccg ctcatccacg tccagttcgt gccgtcccac ttccagaggt cgttgaggtc
     2761 accgaccccc gtcgccccgt agccgtagcc cccgaagagc cagaagtccc cggccgcgtc
     2821 cgtccaggag acggccccct ctcgcgctcc cggcacgttg ccgggcgccg cgagcccctt
     2881 cgtcccgtac gtcccgggct ggtccgagac gttcgatccg ctcatccagg tccagctcgt
     2941 cccgtcccat ttccagaggt cgttgaaacg atagaacgac gtctcccaga cccagtactc
     3001 ctcgaacacc cctccgaaga gccagaggtt ccctccggca tccgtccagg agacggcgcc
     3061 gtaccgcgct ccggggacgt tcgtcggagc cgcgataccc ttcgtcccgt aggtcccgag
     3121 ctggttcgga acgttcgagc cgctcatcca ggtccagttc gtcccgtccc atctccagag
     3181 gtcgttgagg aatccttgga tggagtagtc gtcgaccgtg gaccctccga agagccagcg
     3241 gccacctcct ccgtccgtcc aggagacgga gctgctccgc cacccggggt ggttttcggg
     3301 cgacgcgacg cctttggtcc cgtaggtccc cgacgcggac tcgccgtcgc cgctggacca
     3361 cgtccaattg gtcccgtccc acttccagag gtcgcccaga ggcctaccgc cgctccccca
     3421 ccgcattcca acgcccccga agagccagag gcccccggtc gcgtccgtcc acgtgatcgt
     3481 attgctgcga gccccgggcg tgttcccggg cgcggggacg tcctgcgttc cgtactcgct
     3541 gtgcgcgtga ctgtcgtccg acccgctcac ccatgtccag ctcgtcccgt cccacctcca
     3601 gaggtcgttg cggcttccca agtcccaaag atcgtcgaac atgcctgtcc aagaggggta
     3661 agtatcgtag ccgcccaaga gccagaggct cccatcggtg tccatccacg aggcggcccc
     3721 cgaacgcgat cccggaacgt ttcccgatgc cgcgattccc ttcgttccgt acgtcccggg
     3781 ttggttcacg gcgtccgagc cgctcatcca ggtccagttc gtcccgtccc acctccagag
     3841 gtcgttgaga aagccgaccc ccgtcgcccc gtagccgaag cctccgaaga gccagacttc
     3901 tccggcggcg ttcgtccacg tgatcgcgct cgagcgcgcg ccgggcgcgt ttccgggggc
     3961 tgcggtcccc ttcgttccgt acgccccggg ttggttcacg gcgtccgagc cgctgatcca
     4021 ggtccagctc gtcccgtccc acttccagag gtcgttgagg cgttcgcctc cctcttgcgc
     4081 ggagcggtag cccccgaaga gccagaggtt cccgctcgcg tccgtccacg aaaccgagcg
     4141 ttctcgcgct ccggggacgt tcgacggcga cggaatcccc ttcgttccgt agatgccggg
     4201 ttggtccaac gtgtccgagc cggcgatcca ggtccagctc gtgccgtccc acctccacag
     4261 gtcgttcagc tgaccgagac catcctgcgt gtagccttgt cccccgaata gccagaaccc
     4321 tccattggcg tcgatccacg agaccgcttc ttttcgtgcg ccggggacgt tcgccggggc
     4381 ggcgatcccc ttcgtcccgt agataccggg ttggttctct tcatcagagc cgctcactgc
     4441 tgtccaagac agctcggttc gacggggatg ccccgcatga atagcggct
//
LOCUS       DAOONL010000214         4453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23575_length_4453_cov_19.459527, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000214 DAOONL010000000
VERSION     DAOONL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23575_length_4453_cov_19.459527"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLP50_16880"
     CDS             complement(<1..326)
                     /locus_tag="PLP50_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPA53269.1"
                     /translation="MNLFRTKPIDQLVAEGADTGKGLKRELTAVDLVALGIGAIIGAG
                     IFATLGTATSGGGTLPPAGPGIVVSILLTALACGFCGLCYAEFASLVPVAGSAYTYSY
                     ATLGELV"
     gene            complement(362..1135)
                     /locus_tag="PLP50_16885"
     CDS             complement(362..1135)
                     /locus_tag="PLP50_16885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPA53270.1"
                     /translation="MARLGGRPERDGPVDRHRRRAGDALKRTKAVFLDAGNTLLAADP
                     PVEAVYGDAFRRWGLSADDSAVHDALRATWGHVAARQARGEERWGGGGGERAFWQDFV
                     QDVWGRLGGGLLPEGLLDDLIVHFARQESWVLYPEVLEVLAALRERGLKLVVVSNWDS
                     TLPALLDRLDLTRFFDEVVVSALVGASKPARAIFERALDAAGVAPHEALHVGDSPSED
                     YEGARSAGLPALLLDRAGVAGDGYETIRTLQEILPRLAP"
     gene            complement(1060..1851)
                     /locus_tag="PLP50_16890"
     CDS             complement(1060..1851)
                     /locus_tag="PLP50_16890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA53271.1"
                     /translation="MPETPGRLTGKKALVTGASRGIGRAIALRLAAEGATVACGVRSE
                     ADGAALVAEIAAAGGSAVPVLLDVTDAASVATGVERAARPERRLDLVVNNAGYTSPTP
                     LSGDERSDRAFERSWDVNVAGTWRVLRAALPFLAAGARIVNLSSVLGRFGAPGQAGYC
                     AAKHAVIGLTRSLALELAPRKVTVNAICPGWVDTEMGRSRIRGFGREVGLDEEAAFAA
                     AGKMAPLGEVLVPGEVAGLVAWLASEDARNVTGQSIVIDGGQVMP"
     gene            1909..2163
                     /gene="moaD"
                     /locus_tag="PLP50_16895"
     CDS             1909..2163
                     /gene="moaD"
                     /locus_tag="PLP50_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HPA53272.1"
                     /translation="MKVRILFFASLRDELGESRTVEVAEALPGETTVAALREAVSALT
                     PAAARLGRRLLVAVNERYAADADAVRPGDTVAFLPPLAGG"
     gene            2166..2600
                     /locus_tag="PLP50_16900"
     CDS             2166..2600
                     /locus_tag="PLP50_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPA53273.1"
                     /translation="MPRLVRVPLEPGALLEAARRDGDGGLALFVGVVRNVNDGRAVSE
                     MEYEAYEPMAEAEMERIETDLAGRFPAVRLLLRHRIGRLRVGEVAVVVAAAAPHREEA
                     FAACRAGIEEIKARVPVWKREWGPDGSAWVDPIELPRRSESC"
     gene            2717..3247
                     /locus_tag="PLP50_16905"
     CDS             2717..3247
                     /locus_tag="PLP50_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA53274.1"
                     /translation="MFVALRRVHVAASDFPRSRRFYGETLGLPEVGGFESDWVEFGLG
                     PVLVKVTPLRAGEVSARNQVALILSTGDLEACLTTLKERGVTVTRGPLEEFTGRSAEI
                     LDPDGYRIVVFQASDLHPDEEWVPMSEVDEKVKEKLAAWRAKEKAKAAAVPKKPAPPA
                     ARATPKKPAPKKAKKK"
     gene            3315..>4453
                     /gene="kbl"
                     /locus_tag="PLP50_16910"
     CDS             3315..>4453
                     /gene="kbl"
                     /locus_tag="PLP50_16910"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPA53275.1"
                     /translation="MSTALIDYCKSEIEALKEARTFKKERVLEGPTGARVRVDGRDVL
                     MLTSNNYLGFANHPRIVEAARKALERWGNGLGSVRFICGTQELHKELERTISAFFGTE
                     DTILYMSCWNANEGLFQPLLGEEDAILTDGLNHASIIDGIRLCKAKRYVLPHGDLAAS
                     EKALRESQAQRRRLYMTDGVFSMEGEIGPIPELVELCAKYDTLLVVDDSHATGVIGAT
                     GRGSAEETGVHGKVAVYTSTLGKAMGSAAGGFTTGPAPLVELLRQRSRTTLFSNALPP
                     AVTASSLEAFRMLLEDPAPVQRLRANAAHFRTKMTEAGFNIPKGVHPIVPVIVGDTAK
                     ALAMSAALFEKGVYVSGFGFPVVPQGHARLRCQISAVHSTADLDEA"
BASE COUNT          646 a         1670 c         1525 g          612 t
ORIGIN      
        1 acgagctcgc cgagcgtcgc gtagctgtag gtgtaggccg accccgcgac cgggacgagg
       61 ctcgcgaact ccgcgtagca gaggccgcag aacccgcagg cgagcgccgt caggaggatc
      121 gagacgacga tgccggggcc cgccgggggg agcgtgccgc cccccgaggt cgccgtcccg
      181 agcgtcgcga agatgccggc gccgatgatc gccccgatcc cgagggccac gaggtcgacg
      241 gcggtcagct cgcgcttcag ccccttcccg gtgtcggccc cctcggcgac gagctggtcg
      301 atcggcttcg tacggaagag gttcatgcgc ggctcctcgt cggttcgcgt tcggcgcggc
      361 gtcagggggc gagtcggggg aggatctcct ggagcgtccg gatcgtctcg tacccgtcgc
      421 cggcgacgcc ggcccggtcg agcaggaggg ccggaaggcc ggcggagcgc gctccctcgt
      481 agtcctccga cggcgagtcg cccacatgga gcgcctcgtg gggcgcgacg ccggcggcgt
      541 cgagcgcccg ctcgaagatc gcgcgggccg gcttgctcgc gccgacgagg gccgagacga
      601 cgacctcgtc gaagaagcgg gtcaggtcga gccggtcgag gagggcgggg agcgtcgagt
      661 cccagttgct gacgacgacg agcttcagcc ccctctcgcg aagggccgcg agcacctcca
      721 gcacctccgg gtagaggacc cagctctcct gccgggcgaa gtggacgatc aggtcgtcga
      781 ggagtccctc ggggagaagg ccgccgccga gccgacccca gacgtcctgc acgaagtcct
      841 gccagaacgc ccgctctcct ccgcccccgc cccagcgctc ctccccccgc gcctgacggg
      901 cggccacgtg cccccacgtc gcccgcaggg cgtcgtgcac ggcgctgtcg tcggccgaca
      961 ggccccacct ccggaacgcg tcgccgtaga ccgcctccac cggcgggtcg gccgcgagga
     1021 gcgtgttccc ggcgtcgagg aagaccgcct tcgtccgctt cagggcatca cctgcccgcc
     1081 gtcgatgacg atcgactggc ccgtcacgtt ccgggcgtcc tccgaggcga gccaggcgac
     1141 gaggccggcg acctcgccgg ggacgagcac ctcgccgaga ggagccattt ttccagccgc
     1201 ggcgaaggcc gcctcctcgt cgaggcccac ctcccggccg aacccccgga tccgcgaccg
     1261 tcccatctcc gtgtcgaccc agcccgggca gatcgcgttg accgtcacct tccgcggcgc
     1321 cagctccagg gcgagggagc gagtgaggcc gatgacggcg tgcttcgcgg cgcagtagcc
     1381 cgcctggccg ggcgcgccga agcgccccag gaccgatgac aggttcacga tccgcgctcc
     1441 ggccgcgagg aacgggagcg cggcgcgcaa gacgcgccac gtgcccgcca cattcacgtc
     1501 ccacgaccgc tcgaaggccc ggtcggacct ctcgtcgccg gagagtggcg tcggcgacgt
     1561 gtagccggcg ttgttgacga cgaggtcgag gcgacgctcc ggccgggccg ccctctccac
     1621 gcccgtcgcg acagaggccg catcggtcac gtcgaggagg accgggaccg ccgaaccgcc
     1681 ggccgccgcg atctcggcga cgagcgcggc gccgtcggcc tcggagcgga cgccgcaggc
     1741 gacggtcgcc ccttccgccg cgaggcggag ggcgatcgcc cgcccgatgc cgcgcgaagc
     1801 ccccgtcacg agagccttct tccccgtcag ccgtcccggt gtctcgggca tggaacctcc
     1861 tgattcggcg ggcattctag aactccgccg acgttaagat ccggaccgat gaaggtccgg
     1921 atcctcttct tcgcgtccct ccgggacgag ctgggcgagt cccgtaccgt cgaagtcgcc
     1981 gaggcccttc ccggcgagac gaccgtcgcc gcgctccggg aggccgtctc ggccctcacc
     2041 cccgccgccg cccggctcgg gcgccgcctt ctcgtcgccg tgaacgagcg ctacgccgcc
     2101 gacgccgacg cggtccgccc cggggacacc gtcgcgtttc tccctccgct tgccggaggc
     2161 tgagcgtgcc ccgcctcgtc cgcgtcccgc tcgagccggg cgccctcctc gaagccgccc
     2221 gtcgcgacgg cgacggcggc ctcgccctct tcgtcggcgt cgtccggaac gtgaacgacg
     2281 ggcgtgccgt ttccgagatg gagtacgagg cctacgagcc gatggccgag gccgagatgg
     2341 aacggatcga gaccgacctg gccgggcgct ttcccgccgt ccgcctcctc ctccggcatc
     2401 ggatcgggcg gctccgggtc ggggaggtgg ccgtcgtcgt cgccgcggct gcgccgcacc
     2461 gcgaagaggc cttcgccgcc tgccgggccg gcatcgagga gatcaaggcg cgagtcccgg
     2521 tctggaagcg ggaatggggg ccggacggaa gcgcctgggt cgacccgatt gagctccctc
     2581 gccgatcgga aagttgttga aaagtcaatc actttctggt aaacgggcgt ttcaggggaa
     2641 cgctccccgg atttcgtccg tgtaaccggc ggtgggcctt tttctcggcc gccgggaaac
     2701 caatcgggag gttgagatgt tcgttgccct ccggcgggta cacgtcgccg ccagcgactt
     2761 tccgcggtct cgccgtttct acggcgaaac gctcggcctt ccggaggtcg ggggcttcga
     2821 gagcgactgg gtcgagttcg gcctcgggcc ggtcctggtc aaggtcactc cgctccgcgc
     2881 cggcgaggtt tcggcccgca accaggtggc cctgatcctc tcgacgggcg atctcgaagc
     2941 ctgcctgacg acgctcaagg agcgcggcgt cacggtgacg cggggcccac tcgaggagtt
     3001 caccgggcgc tcggccgaga tcctcgaccc cgacggctat cggatcgtcg tcttccaggc
     3061 ctccgacctc caccccgacg aggagtgggt cccgatgtcg gaggtcgacg agaaggtgaa
     3121 ggagaagctg gcggcctgga gggccaagga gaaggcgaag gccgccgccg tgccgaagaa
     3181 gcccgccccg cccgccgcca gggcgactcc gaagaagccc gcgccgaaga aggcgaagaa
     3241 gaagtagccc tcgcgcccct cgaggcggcc ccgagcgccc cgcaccccgc ggggcgctgg
     3301 tattctggcc ggtcatgagc accgccctca tcgactactg caagtccgag atcgaggccc
     3361 tgaaggaggc ccgcaccttc aagaaggagc gcgtcctcga agggcccacc ggggcccgcg
     3421 tccgcgtcga cggccgcgac gtcctgatgc tcacctccaa caactacctc ggcttcgcga
     3481 accacccgcg gatcgtcgag gccgcccgca aggccctcga gcgctggggg aacggcctcg
     3541 gctccgtccg tttcatctgc ggcacgcagg agctccacaa ggagctcgag cggacgatct
     3601 ccgccttctt cggcaccgag gacacgatcc tctacatgtc gtgctggaac gcgaacgagg
     3661 ggctcttcca gcccctcctg ggcgaggagg acgccatcct gacggacggc ctcaaccacg
     3721 catccatcat cgacggcatc cgcctctgca aggcgaagcg ctacgtcctt ccgcacggcg
     3781 atctggcggc gtcggagaag gcgctccgag agtcgcaggc ccagcgccgg cgcctctaca
     3841 tgaccgacgg cgtcttctcg atggagggcg agatcgggcc gatccccgag ctcgtcgagc
     3901 tctgcgcgaa gtacgacacc ctccttgtcg tcgacgactc ccacgccacc ggcgtcatcg
     3961 gcgccacggg ccgcggctcg gcggaagaga ccggcgtcca cgggaaggtc gccgtctaca
     4021 cctcgacgct cggcaaggcg atgggctcgg ccgccggcgg cttcacaacc ggcccggcgc
     4081 ccctcgtcga gctcctccgc cagcgttcgc ggacgaccct cttctcgaac gctctcccgc
     4141 cggccgtcac cgcgtcctcg ctcgaggcgt tccggatgct cctcgaggac ccggcccccg
     4201 tgcagcgtct ccgcgcgaac gcggcgcact tccggacgaa gatgaccgag gcgggcttca
     4261 acatcccgaa gggcgtccac ccgatcgtcc ccgtcatcgt cggcgacacg gcgaaggccc
     4321 tcgcgatgtc ggccgccctc ttcgagaagg gcgtctacgt ctccggcttc ggcttccccg
     4381 tcgtcccgca ggggcacgcc cgcctgcgct gccagatctc ggccgtccac tccaccgccg
     4441 acctcgacga ggc
//
LOCUS       DAOONL010000215         4417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23824_length_4417_cov_15.514214, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000215 DAOONL010000000
VERSION     DAOONL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23824_length_4417_cov_15.514214"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLP50_16915"
     CDS             complement(<1..700)
                     /locus_tag="PLP50_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPA53276.1"
                     /translation="MARTPNLDDDLDFLLDRALRGEGEIDESLRARLSQAFPALRVPL
                     PPAEGRAAAPKTPAAPPAAHHAAESTGPIEAPGANAPSFAEALPLGIVLSDASGLVRS
                     ANAMARRIFGADAVALEGRRVIELFEAVERAQLAGLLEDAFRGRPTREVGLNAANPAG
                     AVAVRVSAAGRFGPDRKLTQVVWTVREAGVNPAALEVHVHSGKQELLAELGMELGREV
                     SPLLVRLTESLVSVQ"
     gene            746..1249
                     /locus_tag="PLP50_16920"
     CDS             746..1249
                     /locus_tag="PLP50_16920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPA53277.1"
                     /translation="MRDVRLPEFPCVVPVEVRFRDLDAMGHVNNAVYLTYFEQARLAF
                     WLAIHPGGAPGEPVAPETIGFVLARAECDYASPVRLGERLLVGCRAGDFGTTSFAFDY
                     RLVAAGGSVDAEVRLVASGRTVQVGWDWKEGRKSPLPEELRRRIDSFQAGVARPGARS
                     GHAVTGP"
     gene            complement(1236..1544)
                     /locus_tag="PLP50_16925"
     CDS             complement(1236..1544)
                     /locus_tag="PLP50_16925"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HPA53278.1"
                     /translation="MKRTFTFEEARKVFPAVRERTRRAIARIEALGEAWEAPAGPEAR
                     AILLSWAREMEELGIEVKGPGLVDFDSGDGYWCWRWPEEALDYYHGYEEGFAGRVRVQ
                     "
     gene            1574..2923
                     /locus_tag="PLP50_16930"
     CDS             1574..2923
                     /locus_tag="PLP50_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53279.1"
                     /translation="MIPALLVYGAAVLLVGLFAARRATRSAESFLLGDRSFGFLPTWA
                     GLTSTVIGGSTTLVLASLVAENGLPGAWLDIAGALGLLGLGLFLARRIRETGEVTLAA
                     VVGRFYGPRVRKVAALLVVLAEIVWFALLAQATETVVSSLTDWPPTPVLVGTALLFVA
                     YTALGGQRAVLQTDLLQLGLMVVGIVGVALPLALTSLSRTGWPEPLLSFPVGDRFGWT
                     DVGALLVLVGLPHAVGSDVWAKLLSARDAATARRAALGAAAAKLVFGAAVVVIAFAGV
                     ALEVGGVPQETFPTTVRLLAGPLLSPLLLVALVATMQASADSVLLSAAAATSHDLLGG
                     APARTSRILVVAYGALGLLVALGMRDVLATFRLGYAIFASSLILPTLVAFAPRLALRP
                     RFAGAAMLLGGATTLGATLLGARLDPVLLGTAVNALVLLPGLSIRGATGESSVSRGR"
     gene            complement(2907..3506)
                     /locus_tag="PLP50_16935"
     CDS             complement(2907..3506)
                     /locus_tag="PLP50_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA53280.1"
                     /translation="MEKKGVGRPRDGNPEETRREILAAASEAFAAAGYVGATTRAVAS
                     RAGVNVATLHYHFGSKEGLYRAVLEAAGRGTLPALPPADGPGTVAGLVGALLRFGRER
                     PRLARLALLHALAGPEGGPAPGEERVVWAEQTLRPYLAGPAGAEAARAIVALVDAALA
                     TSGAPALDGTAAGEGIESRSPGRQERAVVAAALCLSGLG"
     gene            3609..>4417
                     /locus_tag="PLP50_16940"
     CDS             3609..>4417
                     /locus_tag="PLP50_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53281.1"
                     /translation="MRRPFAAALLLALGGAASADTPATTQWVLATAKATGAGGEQFVS
                     SLRISNPFPQVATVDLTYLAQSQIDPTGAATGDNAAAPKVRVTVGPGETLAVEDVLGT
                     TFAGKAAPFGISAGGLRVDSDVPVSVLSRTYVANARSATGVPGTFGFSIPAQVEAQTL
                     SAGETGWLTFVSGSPSSASGFRTNLILLNTGATPALLRVALRRGDGSVAGERDYTLGR
                     FSAAQQGNLGAAFGLATSETNLRATVTVTSGGPVAIGASLIDNAISSIAYLP"
BASE COUNT          473 a         1721 c         1562 g          661 t
ORIGIN      
        1 gctggaccga gacgagcgac tcggtgaggc ggacgaggag cgggctcacc tcgcgtccca
       61 gctccatgcc gagctcggcg aggagctcct gctttccgct gtggacgtgg acctcgaggg
      121 cggccgggtt cacgcccgct tcccggaccg tccagacgac ctgcgtcagc ttccggtccg
      181 gaccgaaccg gcccgccgcc gagacgcgga cggcgaccgc ccccgccggg ttcgcggcgt
      241 tgagcccgac ctcgcgcgtc ggacgcccgc ggaaggcgtc ctcgaggagt cccgcgagct
      301 gggctcgctc gaccgcctcg aagagctcga tgacgcgccg cccttcgagc gcgaccgcgt
      361 cggcgccgaa gatccggcgc gccatcgcgt tcgcgctccg gacgagaccc gaggcgtcgg
      421 agaggacgat gccgagcggg agcgcttcgg cgaagctggg tgcgttcgcg cccggtgcct
      481 cgatcggtcc ggtcgactcc gccgcgtgat gggcggcggg aggcgcggcc ggcgtcttcg
      541 gcgccgcggc acgtccctcg gcgggcggga gcgggacccg gagcgccggg aacgcctgag
      601 agaggcgcgc gcgcaagctc tcgtcgatct ccccctctcc gcgcagtgcg cggtcgagga
      661 ggaagtcgag gtcgtcgtcg aggttgggtg ttcgtgccac cggacccgta ttctcacccg
      721 aagcggggtg ctagagtcga tccggatgcg cgacgtccga cttcccgagt tcccgtgcgt
      781 cgttcccgtg gaggtccggt tccgcgacct cgacgccatg gggcacgtca acaacgcggt
      841 ctacctcacc tacttcgagc aggcccgcct cgcgttctgg ctggcgatcc acccgggggg
      901 cgcgccgggc gagccggtcg cgccggagac gatcggcttc gttttggcgc gcgccgagtg
      961 tgactacgcc tctccggtcc gcctcggcga gcggctgctc gtcgggtgcc gggcagggga
     1021 tttcgggacg acgtccttcg cgttcgacta ccggctcgtc gcggccgggg gctcggtcga
     1081 cgccgaagtc cggctcgtcg cctcgggccg gacggtgcag gtcggctggg actggaagga
     1141 ggggcggaag tcgcctctcc cggaagagct gcgccggagg atcgactcgt tccaggcggg
     1201 cgtcgcccgc cccggggccc gttcgggcca cgcggtcact ggaccctgac ccgccccgcg
     1261 aacccctctt cgtacccgtg gtagtagtcg agcgcctcct cgggccaccg ccagcaccag
     1321 tagccgtcgc ccgagtcgaa gtcgacgagg ccggggccct tcacctcgat cccgagctcc
     1381 tccatctcgc gggcccagga gaggaggatc gcccgggcct ccggtccggc cggagcctcc
     1441 caggcctcgc cgagcgcctc gatccgtgcg atcgcgcgcc gcgtccgctc gcggacggcc
     1501 ggaaagacct tccgggcttc ctcgaaggtg aacgtcctct tcacgatccg atcgtacccc
     1561 gcggagtccg ctcgtgatcc cggcgctcct cgtctacggg gcggccgtcc tcctcgtcgg
     1621 cctcttcgcc gcccgccggg ccactcgctc cgcggagtcg ttcctcctcg gcgaccgctc
     1681 cttcggcttc ctcccgacct gggcgggcct cacctccacg gtgatcggcg gctcgacgac
     1741 gctcgtcctc gcgtcgctcg tcgcggaaaa cggcctgccg ggcgcctggc tcgacatcgc
     1801 cggagctctc ggcctcctcg gcctcgggct cttcctcgcc cgtcgcatcc gcgagacggg
     1861 cgaggtgacg ctcgcggcgg tcgtcggccg gttctacggg ccgcgcgtcc ggaaggtcgc
     1921 ggccctcctc gtcgtcctcg ccgagatcgt ctggttcgcg ctcctcgcgc aggcgacgga
     1981 aaccgtggtc agctcgctga cggactggcc gccgacgccc gtcctggtcg ggacggcgct
     2041 gctcttcgtg gcctacacgg ccctcggcgg acagcgcgcc gtcctgcaga ccgacctgct
     2101 ccagctcggt ttgatggtgg tcgggatcgt cggcgtcgcg ttgccgctcg cgctcacctc
     2161 tctctcccgg acgggctggc cggagccgct cctttccttc ccggtcgggg accggttcgg
     2221 ctggaccgac gtcggcgcgc tcctcgtcct ggtgggcctt ccgcacgcgg tcgggagcga
     2281 cgtctgggcg aagctcctct cggcgcgcga cgcggcgacg gcccggcgcg ccgcgctcgg
     2341 cgcggccgcc gcgaagctcg tgttcggcgc cgcggtcgtc gtgatcgcgt tcgccggggt
     2401 ggccctcgag gtcgggggcg tgccccagga gaccttcccg acgacggtcc gcctcctggc
     2461 cggcccgctc ctctcgccgc tcctcctcgt ggcgctcgtc gcgacgatgc aggcctctgc
     2521 cgactcggtc ctcctgtcgg ccgcggccgc cacctcccac gacctcctcg gcggggcgcc
     2581 cgcccgaacg tcccgcatcc tcgtcgtggc ctacggcgcg ctcggcctcc tcgtcgcgct
     2641 cgggatgcgc gacgtcctcg ccacgttccg cctcggctac gcgatcttcg cctccagcct
     2701 gatcctcccg acgctcgtcg cgttcgcccc ccggctcgcg ctccggccgc ggttcgcagg
     2761 ggcggcgatg ctcctcggcg gagcgacgac gctcggcgcg acgctcctcg gcgcgcggct
     2821 cgacccggtc ctcctcggga cggccgtgaa cgctctcgtc ctcctcccgg ggctctcgat
     2881 ccggggcgcg acgggcgagt cgtccgtcag ccgaggccgc tgaggcagag cgccgccgcg
     2941 acgaccgcgc gctcctggcg gcccggagag cggctctcga ttccctctcc ggccgccgtc
     3001 ccgtccagcg cgggcgcccc gctcgtcgcc agggccgcgt cgacgagcgc gacgatggcc
     3061 cgggccgcct cggctcccgc cggaccggcg aggtacggtc ggagcgtctg ctccgcccag
     3121 acgacccgct cttctccggg agcgggacct ccctccggcc ccgcgagcgc gtggaggagc
     3181 gcgaggcgcg cgaggcgggg gcgctcccgt ccgaagcgga ggagagcgcc gacgaggccc
     3241 gcgaccgtcc cgggaccgtc ggcggggggc agggccggca gcgtgccccg gccggcggcc
     3301 tcgaggaccg cccggtagag cccctccttg ctgccgaagt ggtagtggag cgtcgcgacg
     3361 ttcaccccgg cgcgggacgc gacggcgcgc gtcgtcgccc cgacgtagcc ggccgcggcg
     3421 aacgcctccg acgcggccgc gaggatctcc cggcgcgtct cttcggggtt gccgtccctg
     3481 ggtcttccga cgcccttctt ttccatcgac cggtttccgt cgggacgcct cctccggatt
     3541 gctcctctcc cgctcgtccc gacgccgcgg tctaagatcg tccgccggcg cccggcaagg
     3601 agatcccgat gagacgcccg tttgccgctg ccctcctcct ggccctcgga ggggctgcct
     3661 ccgcggacac ccccgccacg acgcagtggg tcctcgccac ggcgaaggcc accggcgccg
     3721 gcggcgagca gttcgtcagc tcgctccgga tctccaaccc gttcccgcag gtcgcgaccg
     3781 tcgacctgac gtatctcgcc cagagccaga tcgacccgac cggggcggcg accggcgaca
     3841 acgcggccgc gccgaaggtc cgcgtgacgg tcgggccggg cgagacgctc gccgtcgagg
     3901 acgtcctcgg gacgacgttc gccgggaagg ccgcgccctt cggcatctcc gccggcgggc
     3961 tccgggtcga ctccgacgtg ccggtctcgg tcctttcccg gacctacgtc gcgaacgcca
     4021 ggagcgccac cggcgtcccg gggacgttcg gcttctcgat ccccgcccag gtcgaggcgc
     4081 agacgctctc ggccggggag acgggctggc tgacgttcgt ctccgggtcg ccttcgagcg
     4141 cgagcgggtt ccggacgaac ctgatcctcc tgaacaccgg cgcgaccccg gctctcctgc
     4201 gggtcgcgct ccggcgcggg gacgggagcg tcgcagggga gcgcgactac acgctcgggc
     4261 gcttctcggc ggctcagcag gggaacctcg gcgccgcctt cggcctcgcg acctccgaga
     4321 cgaacctccg ggcgaccgtg accgtgacga gcggagggcc ggtcgcgatc ggcgcctccc
     4381 tcatcgacaa cgcgatctcg tccatcgcct acctgcc
//
LOCUS       DAOONL010000216         4415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23843_length_4415_cov_23.318807, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000216 DAOONL010000000
VERSION     DAOONL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23843_length_4415_cov_23.318807"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PLP50_16945"
     CDS             <1..1605
                     /locus_tag="PLP50_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPA53282.1"
                     /translation="ASDGGLCPLGLTAHHLAKHPLRAVAPERVSGGKRETIDRAAASA
                     ALAAAISAAAKDPKRSLLVVDGRPGRSLSHAFRALAAATNGRYAVPGSPSPALDVLAA
                     RFDGPLSLGVDLSKARRVVSFGMPVLTDAGTPGLLARLRAEGRRKPKNERLEVVHVGA
                     TRTKSAGFADRFVPIAPGTEAALALGLAHVLLDEKLADAAFLAARTTGLAGLEALAAR
                     FAPPLVEQKTGVPAGDVVALARDLAASRPAVVLGGDPAEPLSAEADAAIAALNLLLGA
                     PAGPLVARRAAPAAPDGDAKLAAVTPLDAVPERSVGLLLLDGTVPEGLVPWARLEKLL
                     ADGAVVASFSAFRAGLGEKATLLVPGPAPLEEAVEIDGPADAPLATFAFAPALLAPPK
                     ELVLPEELLREAAAAAGFELPASPAGRAAAIVAAKRGQLFDPSTATTTPVSSIADAAA
                     LETAFAAGACWVDDPAPAPAGRFALLRDEEAPRLLESALGAPGPEPVRVAALPRTALS
                     AAPSSPLLTKLTRETALFPAPSAAKA"
     gene            1608..2366
                     /locus_tag="PLP50_16950"
     CDS             1608..2366
                     /locus_tag="PLP50_16950"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPA53283.1"
                     /translation="MTATKQTSTLPTHRYTLVVDLDRCNGCGSCATACAVENNIPPAP
                     ERSNVRTGVVWLRVHDVATVDPSGRSTFVPIPCQQCDEPPCVDVCPQRAVEYDPRTGI
                     VGQIPVRCLGCRYCMVACPYHTRSFNWWSPEWPEGMKASLNPDVSIRQRGVVEKCNFC
                     VQRLQAARAKAAAAGQADIDPADYVPACVEACPSRALSFGDANDPDGEVAKLAKDPES
                     FRLLAALGTGPKVLYRSSDPVVRERLSRTGVTRG"
     gene            2359..3594
                     /gene="nrfD"
                     /locus_tag="PLP50_16955"
     CDS             2359..3594
                     /gene="nrfD"
                     /locus_tag="PLP50_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPA53284.1"
                     /translation="MDDTLIARGLKRTPLPRFLLWLLPWAGVLAFGLYAVYLCFALGL
                     NQTNMDNRFAFGLWIYLDLTVIALGAGAFFTGFLLYILKRKELKAVINSAVVIGFICY
                     SGAVGILMVDVGQPLRAWFTFWHPNVHSMLTEVTFCITIYLCVLAIEYLPLVLKNRRL
                     SEVPGLLVLEHQLHKLMVVFAGVGTLLSFFHQGSLGGLYGVLRGNPFAYREHIFIWPT
                     TFFLFVLSAAAVGPSFILLTTWLVGKITRKQLVAPEVLQTLGKISGWLLLVYVVLKGI
                     DSLVWLNSTSPQTGVQVSSFYANPPFGIQVLFAEVVLLGLVPAVLLLSRRVRARTGLL
                     VAVAATACAGVVLNRYVMTIQTLALPSLPFDSFASYTPSWQEVAAFLAVLGYGVLLYS
                     ISFRWLNLFPQERELATKG"
     gene            3608..>4415
                     /locus_tag="PLP50_16960"
     CDS             3608..>4415
                     /locus_tag="PLP50_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53285.1"
                     /translation="MLPGNTDFIADPFNIAFLGLFALILTAVGVTLFRVGLRTRETIA
                     RGKAASMLWHEEFEELPASARGCRHAMTGELPGRTCARAFDCKGCETHAKLVAEGKVL
                     PPADAAGFDVPTDRLYHRGHAWLKPLEDGTVAIGLDDLATRLVGKPDAVVLPAVGSSL
                     AENGPALRLRAGKARVRVASPVSGEVVEHGSLSSDFLLRVKPEGPLDTRALLTPREAA
                     AYFSRELDRLQLALSGSVPALADGGLPVGDLSKAIPEDDRDRVLGEMLLEA"
BASE COUNT          520 a         1869 c         1356 g          670 t
ORIGIN      
        1 gcgagcgacg gcggcctctg cccgctcggg ctgaccgccc accacctcgc gaagcacccg
       61 ctccgcgccg tcgcccccga gcgcgtctcg ggcgggaagc gcgaaacgat cgaccgcgcc
      121 gccgcgtccg cagccctcgc cgccgcgatc tccgcggccg cgaaggaccc gaagcgctcc
      181 ctcctcgtcg tcgacggccg tcccggccga agcctctcgc acgccttccg ggctcttgcc
      241 gcggcgacga acgggcgcta cgccgttccc ggatctccct cgccggccct cgacgtcctc
      301 gcggcccgct tcgacggccc gctctcgctc ggcgtcgacc tctcgaaggc ccgccgcgtc
      361 gtctcgttcg ggatgccggt cctgacggac gccgggacgc ccggactcct cgcgcgcctc
      421 cgcgccgagg ggcgccggaa gccgaagaac gagcggctcg aggtcgtcca cgtcggggcg
      481 acccggacga agagcgccgg cttcgccgac cgcttcgtcc cgatcgcccc cggtaccgaa
      541 gccgctctcg ccctcggcct cgcccacgtc ctcctcgacg agaagctcgc cgacgcggcg
      601 ttcctcgccg cgcgaacgac gggcctcgcc gggctcgagg cgctcgcggc ccgcttcgcc
      661 ccgcccctcg tcgagcagaa gacgggcgtc cccgcgggcg acgtcgtcgc gctcgcgcgc
      721 gacctcgcgg cgagccgccc ggccgtcgtc ctcggaggcg acccggccga gccgctctcc
      781 gccgaggccg acgccgcgat cgccgccctg aacctcctcc tcggcgcccc ggccggcccg
      841 ctcgtcgcgc gccgcgcggc gcccgccgct ccggacggcg acgcgaagct cgccgcggtc
      901 acccctctcg acgctgtgcc cgagcgctcc gtcggcctcc tcctcctcga cggtacggtc
      961 cccgaggggc tcgtcccctg ggcgcgactc gagaagctcc tcgccgacgg cgccgtcgtg
     1021 gcgagcttct ccgcgttccg cgccggcctc ggcgagaagg cgacgctcct cgtacccggt
     1081 ccggcgccgc tggaagaggc ggtcgagatc gacggccccg ccgacgcgcc gctcgcgacg
     1141 ttcgccttcg cccccgccct cctcgctccg ccgaaggagc tcgtcctccc cgaggagctc
     1201 ctccgcgagg cggccgccgc cgcgggcttc gagctcccgg cgtctccggc cggccgtgcc
     1261 gcggcgatcg ttgcggcgaa gcgcgggcag ctcttcgacc cctccacggc gacgacgacc
     1321 ccggtctcct cgatcgccga cgccgcggcc ctcgagacgg ccttcgcggc cggcgcctgc
     1381 tgggtcgacg acccggcccc cgctcccgcg gggcgcttcg cgctccttcg cgacgaggag
     1441 gcgccgcgcc tcctcgagtc ggccctcggc gcgcccggcc ccgagccggt ccgcgtcgcg
     1501 gccctcccgc gcacggccct ctccgccgcc ccctcctccc cgctcctcac gaagctgacc
     1561 cgcgagacgg cgctcttccc cgcgccctcc gccgcgaagg cgtgaccatg accgccacca
     1621 agcagacctc cactctcccg acccaccgct acacgctcgt cgtcgacctc gaccgctgca
     1681 acggctgcgg ctcctgcgcg accgcctgtg ccgtcgagaa caacatcccg cctgctcccg
     1741 agcgctcgaa cgtgaggacc ggcgtcgtct ggctgcgcgt ccacgacgtc gccaccgtcg
     1801 atccctcggg gcgctcgacg ttcgtcccaa tcccctgcca gcagtgcgac gagcccccct
     1861 gcgtcgacgt ctgcccccag cgcgcggtcg agtacgaccc gcgcaccggc atcgtcggcc
     1921 agatccccgt ccgctgcctc ggctgccgct actgcatggt cgcctgcccc taccacacgc
     1981 gctcgttcaa ctggtggagc ccggagtggc ccgaggggat gaaggcctcc ctcaaccccg
     2041 acgtctcgat ccgccagcgc ggcgtcgtcg agaagtgcaa cttctgcgtc cagcgcctcc
     2101 aggcggcccg cgcgaaggcg gcggccgccg ggcaggccga catcgacccg gccgactacg
     2161 tccccgcctg cgtcgaggcg tgcccgtcgc gggcgctctc cttcggcgac gcgaacgacc
     2221 cggacggcga ggtcgcgaag ctcgcgaagg acccggagag cttccgcctc ctcgccgccc
     2281 tcgggaccgg accgaaggtc ctctaccgct cgtcggaccc cgtcgtccgc gagcgcctct
     2341 cccgcacggg ggtgacgcgt ggatgacacc ctgatcgccc gcggcctgaa gcggaccccg
     2401 ctgccgcgat tcctcctctg gctccttccc tgggcgggag tcctcgcctt cggcctctac
     2461 gccgtctacc tctgcttcgc cctcggcctg aaccagacga acatggacaa ccggttcgcg
     2521 ttcggcctct ggatctacct cgacctgacg gtcatcgcgc tcggcgccgg cgccttcttc
     2581 acgggcttcc tcctctacat cctgaagcgg aaggagctga aggcggtcat caacagcgcc
     2641 gtcgtcatcg gattcatctg ctacagcggc gccgtcggca tcctgatggt cgacgtcggc
     2701 cagccgctcc gcgcctggtt cacgttctgg cacccgaacg tccactcgat gctgaccgag
     2761 gtgacgttct gcatcacgat ttacctctgc gtcctggcga tcgagtacct gccgctcgtc
     2821 ctgaagaacc ggcggctcag cgaggtcccg gggctcctcg tcctcgagca ccagctccac
     2881 aagctgatgg tcgtcttcgc cggggtcggg acgctcctct ccttcttcca ccagggctcg
     2941 ctcggcggcc tctacggcgt cctccgcggg aacccgttcg cctatcgcga gcacatcttc
     3001 atctggccga cgacgttctt cctcttcgtc ctctccgccg cggccgtcgg cccgagcttc
     3061 atcctcctga cgacctggct cgtcgggaag atcacccgca agcagctcgt tgcccccgag
     3121 gtcctccaga cgctcgggaa gatctcgggc tggctcctcc tcgtctacgt cgtcctgaag
     3181 gggatcgact ccctcgtctg gctgaactcg acgagcccgc agaccggcgt ccaggtctcg
     3241 tccttctacg cgaacccgcc gttcggcatc caggtcctct tcgccgaggt cgtcctcctc
     3301 ggcctcgtcc cggcggtcct cctcctctcg cggcgcgtcc gcgcgcggac gggcctcctc
     3361 gtcgcggtcg ccgcgacggc ctgcgccgga gtcgtcctga accgatacgt catgacgatc
     3421 cagacgctcg cgctcccctc gctcccgttc gacagcttcg cgtcctacac gccgtcctgg
     3481 caggaggtcg cggccttcct cgccgtcctc ggctacggcg tccttctcta ctcgatctcc
     3541 ttccgctggc tgaacctctt cccccaggag agggagctgg cgacgaaggg ctgacggagg
     3601 ttccgagatg ctgcccggaa acaccgactt catcgccgac cccttcaaca tcgccttcct
     3661 cggcctcttc gcgctcatcc tgaccgcggt cggcgtgacg ctcttccgcg tcgggctccg
     3721 gacccgcgag acgatcgcgc gcggcaaggc cgcctcgatg ctctggcacg aggagttcga
     3781 ggagctcccg gcgtcggccc gcggctgccg ccacgcgatg accggagagc tcccggggcg
     3841 cacctgcgcc cgcgccttcg actgcaaggg gtgcgagacg cacgcgaagc tcgtcgccga
     3901 ggggaaggtc ctcccgcccg ccgacgccgc cggcttcgac gtcccgaccg accgcctcta
     3961 ccaccgcggg cacgcctggc tgaagccgct cgaagacggc accgtcgcga tcggcctcga
     4021 cgacctcgcc acgcgcctcg tcggcaagcc cgacgcggtc gtcctcccgg ccgtcggctc
     4081 ctctctcgcc gagaacggcc cggccctccg cctgcgcgcc ggcaaagcgc gcgtccgagt
     4141 cgcctccccc gtcagcggag aggtcgtcga gcacggctcg ctctcctcgg acttcctcct
     4201 ccgcgtgaag cccgaaggcc ccctcgacac ccgcgcgctc ctgactcccc gcgaggccgc
     4261 cgcctacttc tcccgcgagc tcgaccgtct ccagctcgcc ctctccggca gcgtccccgc
     4321 cctcgccgac ggcggcctcc cggtcggcga cctctcgaag gccattcccg aagacgaccg
     4381 cgaccgcgtc ctcggcgaga tgctcctgga agcct
//
LOCUS       DAOONL010000217         4404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_23920_length_4404_cov_15.181421, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000217 DAOONL010000000
VERSION     DAOONL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23920_length_4404_cov_15.181421"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLP50_16965"
     CDS             <1..861
                     /locus_tag="PLP50_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPA53286.1"
                     /translation="LVASVSRLCAMNLLLHGIGPSAGEDVEPPISTDDSLRSHPGKTW
                     DLVLTNPPFGKKSSVSFVNEEGEEERDALTYLRDDFWASTSNKQLNFVQHIRSILKMN
                     GRAAVVVPDNVLFEGGAGETIRRRLLADCDVHTLLRLPTGIFYAQGVKANVLFFDRKP
                     AAEKPWTKKLWIYDLRTNQHFTLKTNPLKREDLDEFVDLYKPENRHARKQTWSEKKPE
                     GRWRVFTYEELLARDKVSLDIFWLRDESLADAENLPDPDVIAGEIAEDLRDALELIGE
                     IAGEVGATKP"
     gene            1893..2261
                     /locus_tag="PLP50_16970"
     CDS             1893..2261
                     /locus_tag="PLP50_16970"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPA53287.1"
                     /translation="MAAAPDWKIDSYDTENGERPAAYFLRSLGAQDRVDAASLLKSLR
                     EQGNALRRPNSASLGEGLFELRGKQVRIFYIFLPGRVALLLDGEIKKRNDIPTATMKR
                     MRKLQKDALAEYGRRLRPRR"
     gene            2265..2645
                     /locus_tag="PLP50_16975"
     CDS             2265..2645
                     /locus_tag="PLP50_16975"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPA53288.1"
                     /translation="MPKRKKTEVEEWLDGVLADPVLASAVEERMAEMKIEQELVALRE
                     REGLSQRDLAMKLGTTQPYIAKLESGRVRNVGLKTLAKYAAATGTTLTVSFTPARQRR
                     KASGTRSTIQRPRRRTTRTRRGVT"
     gene            2784..2873
                     /locus_tag="PLP50_16980"
     tRNA            2784..2873
                     /locus_tag="PLP50_16980"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2847..4211
                     /locus_tag="PLP50_16985"
     CDS             2847..4211
                     /locus_tag="PLP50_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53289.1"
                     /translation="MRVRVPPPADAREANGPVALSTRAILAFWLPLAATWLMMSVEGP
                     YLSAIVARLVDPVPNLAAFGVAFTLAWLAESPIIMLLTATTALAKDRASFVAFRRFAT
                     RLNGAVTAALLVLVLPPVFRFVAERLLALPPEVARLAHLATALLVPWPAAIGYRRFYQ
                     GVLVRNRMTRRVAYGTVVRLATMSATAAALATATRLPGALIAAAALSVGVVAEAAASR
                     FMARHLVAGLLSENGDAPPPPQRELARFYFPLALTSMISMTTAPLLTFFMSHGPNAVP
                     SLAAWPVVSALVFLFRSGGVGFQETSVALSGEAGENVPAVRRTGALLGAGASLLLAAV
                     AFSPLLGLWYRVVVGLGPELIPVAVVPTRILVLLPALEYALSFLRSRWVLARQTRPVT
                     VATALEAAGLLSGMLLLVVVARVPGTIAAALALLLGRLAACAFLAGLGGTARARRAFA
                     SARR"
BASE COUNT          687 a         1510 c         1556 g          651 t
ORIGIN      
        1 ctcgtcgcct ccgtgtcgcg cctctgcgcc atgaacctcc tcctgcacgg catcggcccg
       61 tcggccggcg aggacgtgga gccgccgatc tcgaccgacg acagtctccg gagccacccg
      121 ggaaagacct gggacctcgt cctgacgaac ccgccgttcg ggaagaagtc gagcgtcagc
      181 ttcgtgaacg aggaagggga ggaggagcgc gacgcgctca cctacctccg cgacgacttc
      241 tgggcctcca cctcgaacaa gcagctcaac ttcgtccagc acatccggtc gatcctgaag
      301 atgaacggcc gcgccgccgt cgtcgtcccc gacaacgtcc tcttcgaggg gggcgccggg
      361 gagacgatcc gccgcaggct tcttgccgac tgcgacgtcc acaccctcct gcggctcccg
      421 accggcatct tctacgccca gggggtcaag gcgaacgtcc tcttcttcga ccgcaagccc
      481 gccgccgaga agccctggac gaagaagctc tggatctacg acctccggac gaaccagcac
      541 ttcaccctga agacgaaccc cctgaagcgg gaagacctcg acgagttcgt cgacctctac
      601 aagcccgaga accgccacgc ccggaagcag acctggtccg agaagaaacc cgagggccgc
      661 tggcgggtct tcacctacga ggagcttctc gctcgcgaca aggtcagcct cgacatcttc
      721 tggctgaggg acgagagcct ggccgacgcc gagaacctgc cggacccgga cgtcatcgcc
      781 ggggagatcg cggaggacct gcgggatgcg ctggaactga tcggggaaat tgccggcgaa
      841 gtgggagcta cgaagccctg aatccggcga ggtgacggcg gcgcgaatca acggaggcgc
      901 cggacaacaa ctggagggac atcgaccgaa gccgagagcc gtagcggcag ggtcggccgt
      961 ggcggcctcg cggcgcgctt tcgatcgggg agcagccctg gtctccaggg tgcctggtgt
     1021 ggagccgcgg cgtcgagtgc gaccgtgatc accgaggagg gaattcccgc gcccttccgc
     1081 ccgaggatgt cgtcagccga gaaggcagga cgaatccggg cctggcggac gtaggcgacg
     1141 aggaggtcct cgctgaggcc ctccttgatc ggccggagga cggcgtcctt ggcctcagcg
     1201 ggggtcactg tcgcgcctac ggtttcggac ttcgggttct tcctgctggc gcacgcagcc
     1261 cgagatccgg gtcgtgcaac cagcagcgcc aggttcttcc gaaaggcgtc gctgggcggg
     1321 cgccacggcg ggcaggagga ggaggagcgt tgcgatcggc gcaagaggac accggctgtc
     1381 gaagcggagc aaggagatct cgttcattgc tcccagccaa ccctctccct aagcgaggga
     1441 gaatggtccg cggctccgcc gtcttcgcgt cgatgacgga ggcataagcg ccgacctggc
     1501 acgtcgcagg cgagcacgac aacacgacac tgccgccgtc gaggttcgtg acgtcaaggt
     1561 cgttagcgag gtccaactgg acgaacccaa gcgggccgac ggtgacggcg cgcgtcttga
     1621 ccgtcgtgcc gtagggccgg aggacctgga gcgtcgtaac ggcttgcgtc tcaccgaagt
     1681 tcgcgagggc tatgttcgtc cggaaatgcg ggtcctgccg aacgccggcg agagccttcg
     1741 gcgaaggtct acagccacgt ggcgatacgg agctcgagga gatccacgcg gccacccgcg
     1801 cggcggcccg gctggggccg acggagggct cccgcacgac tcggggtgac ggatcgcgcg
     1861 ataacctata agatatctaa gaaggaggcg ccttggccgc agctccggac tggaagatcg
     1921 attcctacga taccgagaac ggcgaaaggc cggcggccta cttcctccgg tcgctcgggg
     1981 cgcaggacag ggtcgatgcg gcctcgctcc tgaagagcct gagagagcag ggaaacgccc
     2041 tccgacgccc caactcggct tcgctcggag aggggctctt cgagctgaga ggcaagcagg
     2101 tccggatctt ctacatcttc ctccccggcc gcgtggcgct cctgctggac ggagagatca
     2161 agaaacggaa cgacatcccg accgcgacga tgaagaggat gaggaagctc cagaaggacg
     2221 ccctggctga gtacggacgg cggcttcgac cgaggaggtg acccatgccg aagaggaaga
     2281 agaccgaggt ggaagagtgg ctcgacgggg tcctggccga ccccgtgctc gcgagcgcgg
     2341 tcgaggagcg gatggcggag atgaagatcg agcaggagct cgtcgcgctg cgcgagaggg
     2401 aagggctctc ccagcgagac ctcgcgatga agctcgggac gacccagcct tacatcgcga
     2461 agctcgagtc cggccgggtc cggaacgtcg gactgaagac cctcgccaag tacgccgccg
     2521 ctaccggcac cactctcact gtgagcttca cgccggcacg gcagcgccgt aaggcgagcg
     2581 ggacccggag cacgatccag cgcccccgcc ggcggacgac ccggacgcga cgcggcgtca
     2641 cctgatctgc tggaaatgac gaaggccaag gaggccggtg gacaggaggc tcgtcgggga
     2701 aggcccgggc ggaggatggc agacgagcga ggctcgcggc cacaggccgg catccttgag
     2761 ccggccgaac cgactccgca cctgccgggg tccgtttcgg gtcagcgaac tgtcgtgaca
     2821 gacgggggac ggggccccga agggccgtga gggttcgagt ccctcctccg gcagacgctc
     2881 gggaggcgaa cgggccggtc gccctctcga cgcgggcgat cctcgcgttc tggctcccgc
     2941 tggcggcgac gtggctgatg atgtcggtgg aggggccgta cctctccgcg atcgtcgcgc
     3001 gcctggtcga cccggtgccg aacctcgcag cgttcggcgt cgcgttcacg ctcgcgtggc
     3061 tcgccgagtc gccgatcatc atgctcctga cggcgacgac ggcgctggcg aaggaccggg
     3121 cgtcgttcgt cgcgttccgg cgcttcgcga cgcgcctcaa cggcgccgtg acggcggccc
     3181 tcctcgtcct cgtcctcccg ccggtcttcc gcttcgtcgc ggagaggctc ctcgcgctgc
     3241 cgcccgaggt cgcacgcctc gcgcacctgg cgacggcgct cctcgtgccg tggcccgccg
     3301 cgatcgggta ccgccgcttc taccaggggg tcctcgtgcg gaaccggatg acgcggcggg
     3361 tcgcgtacgg cacggtcgtc cggctcgcga cgatgtccgc gacggcggcc gcgctcgcga
     3421 cggcgacccg gctccccggg gcgctgatcg ccgcggcggc gctgagcgtc ggggtcgtcg
     3481 ccgaggcggc ggcgagccga ttcatggcgc gtcatctcgt ggcggggctc ctgtcggaga
     3541 acggggacgc ccctcctccg ccgcagcgag agctcgcgcg cttctacttc ccgctcgccc
     3601 tcacgtcgat gatctcgatg acgacggcgc cgctcctgac gttcttcatg agccacggcc
     3661 cgaacgcggt gccgtcgctc gcggcgtggc cggtcgtgag cgccctcgtc ttcctcttcc
     3721 ggagcggggg cgtcgggttc caggagacga gcgtggcatt gagcggggag gcgggagaga
     3781 acgtcccggc ggttcgccgg acgggggcgc tcctcggagc gggagcgtcg ctcctcctcg
     3841 ccgcggtcgc gttctcgccg ctcctcgggc tctggtaccg cgtcgtcgtc gggctgggac
     3901 ccgagctgat cccggtcgcc gtcgtgccga cgcggatcct cgttctcctc ccggcgctcg
     3961 agtacgcgct ttcgttcctc cgatcccgct gggtcctcgc gcggcagacg cgacccgtga
     4021 cggtcgccac cgccctcgag gccgccgggc tcctgtccgg catgctgctc ctcgtcgtcg
     4081 tcgcgagggt tccggggacg atcgccgcgg ctctcgccct cctgctcggg cggctcgccg
     4141 cctgcgcgtt cctcgccgga ctcggcggga ccgcgcgggc tcgacgggcg ttcgcgagcg
     4201 cgcgtcgctg agccgcccgg ggcgaaacgg tggcgcccgg acgcccgtcg tggtattccg
     4261 gccggtcaag gaggccgtga tgcccttccg cctgctcgcc ctgctcccgg cgctcgccct
     4321 cgccctgccc gccctcgcgc agtcgaagct cctcgtcgtc ctcaggaacg gagaggttcg
     4381 aaccttcgag ctggccgagg tgga
//
LOCUS       DAOONL010000218         4328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_24470_length_4328_cov_13.122630, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000218 DAOONL010000000
VERSION     DAOONL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24470_length_4328_cov_13.122630"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLP50_16990"
     CDS             <1..906
                     /locus_tag="PLP50_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPA53290.1"
                     /translation="DGRFEAGQSIRIQQIRSAMCVPLWNQDRVIGALHVDTPLRVGSF
                     TADDLDLLTALGNFAAVAIERARLQRRIEREERIRERLSRYHSPGVVEEIASDASGVI
                     ERVRTRNVTVFFADIVGFTSAAEGMEPEALSRFLSTFFTYAADAIFAQGGTLDKFIGD
                     AVMAFFGAPIVQPDHARRAVAAAAKLVENVKAWNLERVARGEPPVGVRIGVNTGNVVV
                     GDIGSAQRVDYTVLGNTVNVAARIEEYVAGPNEVAVGEETARLTAEDYAFEPLGDVKL
                     KGLSRGLPCYRVKLDARGVPLPAPR"
     gene            907..>1416
                     /locus_tag="PLP50_16995"
     CDS             907..>1416
                     /locus_tag="PLP50_16995"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPA53291.1"
                     /translation="MPPSLDHLVFLTSNAGKAREASALLGRAVEARALDLPEIQSLDF
                     VEVVTAKALAAAEQLGVPVLVEDSGLALPGWKGYPGPLTKYAVGAVGEAGFARLAHAW
                     GDARAEAVSTLGLAWPGAAPEDVVVAVGRVAGSLAPEPRGASGFGWDVIFVPDGETRT
                     FAQMSAEEKN"
     assembly_gap    1417..1516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1517..1912
                     /locus_tag="PLP50_17000"
     CDS             <1517..1912
                     /locus_tag="PLP50_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53292.1"
                     /translation="WGSAPVEGMQLVAEDGEHRFLSRSTDSLRIGRVVLTSITYVYQG
                     GRLQAAVIELPASGFEPLQRQLREDWGAPRTLKPDQKYAWADARAGDEATLAILEKLP
                     ASRTAKLVVSAKAMSAARARESGAADAGA"
     gene            2058..2777
                     /locus_tag="PLP50_17005"
     CDS             2058..2777
                     /locus_tag="PLP50_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53293.1"
                     /translation="MRARTGRIVVIALAALLLAPPSLAKEEKKVFIRTEVESTNGDPI
                     RVLLVSDVTRMDRSEIRSVQLWDAGSGFRFLVLTHVAAPTSWSYTLIGPKERQVVTFR
                     KDPKDPSLTPKELARSTGVLAVGDRELVLHSDDAGAPAVSARVEKLLLDVFDPGELSA
                     IRAAVGLVWRCSVWIPRGRLLPVIAPYAHRRDGRPCTAAFRDVPADERDRKWELSFVT
                     REPAVDEFLKADAGIARGVRK"
     gene            2774..3550
                     /locus_tag="PLP50_17010"
     CDS             2774..3550
                     /locus_tag="PLP50_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53294.1"
                     /translation="MTRGLRPLLAAVLFTTAIGARGEEPTPWSGPVRTGTILAGDVAL
                     FSYELRETDPLKLPLVRELVVCRPRGELYRLRVTSRKGDFALELGYHSRVKPVEPEVF
                     IGPVPTKDAPIPRVPEDPTGLFTVPLTKLRAGSEELEFFVPMDFFVPTVQRWLEETWK
                     GTSRDLPQALNDVLVASRLVPFGMGEELTSLLGVAPTAELSQALIDVLARPKSEASGK
                     VEGRTMKSFFQLLPKAELKDYAEGRKLRRVERSPEYCSGP"
     gene            complement(3569..4186)
                     /gene="coaE"
                     /locus_tag="PLP50_17015"
     CDS             complement(3569..4186)
                     /gene="coaE"
                     /locus_tag="PLP50_17015"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPA53295.1"
                     /translation="MLRVGLTGGIASGKSAVGSRWRALGIPVLDADRLVHALYEPGAP
                     GAVAVAEEFGAELLDERGAVDRPRLARLAFAEPATVARLNARIHPIVRVETDRWLAER
                     EAEGHPVAVVEATLLVENHGRDRYDVLVAASAPEELRLARALSRDPGATRDAVLARMR
                     AQLPDAERNAACDEVIVTDGTLEELRTKAEAVARRLRSRASGRRA"
     gene            complement(4189..>4328)
                     /locus_tag="PLP50_17020"
     CDS             complement(4189..>4328)
                     /locus_tag="PLP50_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HPA53296.1"
                     /translation="VGDVHPVDVAERASRYTPVPGGVGPLTIARLLANTVDLCRARRR
                     L"
BASE COUNT          604 a         1464 c         1555 g          605 t
ORIGIN      
        1 gacggccgct tcgaggcggg gcagtcgatc cggatccagc agatccgctc ggcgatgtgc
       61 gtcccgctct ggaaccagga ccgggtcatc ggcgcgctcc acgtcgacac gccgctccgg
      121 gtcgggagct tcaccgccga cgacctcgac ctcctgaccg ccctcggcaa cttcgccgcc
      181 gtcgccatcg agcgggcgcg cctccagcgc cggatcgagc gggaggagcg gatccgcgag
      241 cgcctctctc gctaccactc tcccggcgtc gtcgaggaga tcgcctccga cgcgagcggc
      301 gtcatcgagc gggtgaggac gcggaacgtc accgtcttct tcgccgacat cgtcggcttc
      361 acgagcgcgg ccgaggggat ggagcccgag gcgctctcgc gcttcctgtc gacgttcttc
      421 acctacgcgg cggacgcgat cttcgcccag ggggggaccc tcgacaagtt catcggcgac
      481 gccgtcatgg cgttcttcgg cgcgccgatc gtccagcccg accacgcgcg ccgggcggtc
      541 gcggcggccg cgaagctcgt cgagaacgtc aaggcctgga acctcgagcg ggtcgcgcgc
      601 ggcgagccgc ccgtcggcgt gaggatcggc gtcaacaccg gcaacgtcgt cgtcggcgac
      661 atcggctcgg cgcagcgcgt cgactacacc gtcctcggca acaccgtgaa cgtcgcggcc
      721 cggatcgagg agtacgtcgc cgggccgaac gaggtcgccg tcggcgagga gacggcccgc
      781 ctgaccgccg aagactacgc gttcgagccg ctcggcgacg tgaagctcaa ggggctttcg
      841 cgcggcctgc cctgctaccg ggtcaagctc gacgcccgcg gcgtccccct gccggcgccg
      901 cgctgaatgc ccccgagcct cgaccacctc gtcttcctca cctcgaacgc cggcaaggcg
      961 cgcgaggcct ccgccctcct cggccgcgcc gtcgaggcgc gggcgctcga cctccccgag
     1021 atccagtccc tcgacttcgt cgaggtcgtc accgcgaagg cgctcgccgc cgcggagcag
     1081 ctcggcgtgc cggtcctcgt cgaggactcg ggcctcgcgc ttcccggctg gaagggctac
     1141 cccggccctc tgacgaagta cgccgtcggc gcggtcggcg aggcgggctt cgcgcgactc
     1201 gcgcacgcct ggggcgatgc gcgcgccgag gcggtctcga cgctcggcct cgcctggccg
     1261 ggcgcggctc ccgaagatgt cgtcgtcgcg gtggggcgcg tcgccggctc gctcgccccc
     1321 gagccgcgcg gcgcgagcgg cttcggctgg gacgtcatct tcgtccccga cggcgagacg
     1381 cggaccttcg cccagatgag cgccgaggag aagaacnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnntggg ggagcgcccc ggtcgagggg atgcagctcg tcgccgagga
     1561 tggcgagcac cgattcctgt ctcgctcgac ggactcgctg aggatcggaa gagtcgtcct
     1621 cacatcgatc acctacgtct accagggcgg gcggctccag gcggccgtca tcgagctccc
     1681 ggcgtcgggc ttcgagccgc tccagcgcca gctccgggag gactggggtg cgccgaggac
     1741 gctgaagccc gaccagaagt acgcgtgggc cgacgcgagg gcgggggacg aggcgaccct
     1801 ggcgatcctc gagaagctgc cggcgagccg gaccgcgaag ctcgtcgtct ccgcgaaggc
     1861 gatgagcgcc gcccgagcgc gggagtcggg cgcggccgac gccggagcgt agacccttcc
     1921 ggacgcggcc ggcgccggag gcccgggagg tcggtgtcga tagcagcgaa cggccttcaa
     1981 gagaggacgt gcccactcgg cggcgaggag tcgtcgcgag tgaagggctc acgcgtggcg
     2041 tatcgtcccg agccgagatg agagcccgta ccggccgaat cgtcgtaatc gcccttgccg
     2101 ccctccttct cgctccgccc tcgctcgcga aggaggagaa gaaggtcttc atccggaccg
     2161 aggtcgaatc gacgaacggc gaccccattc gtgttctcct ggtgtcggac gttactcgaa
     2221 tggacaggtc cgagattcga agcgtacagc tctgggacgc cggatcgggt tttcgtttcc
     2281 tcgtcttgac gcatgtcgct gcgccgacga gttggtccta cacgttgatc gggccgaaag
     2341 agcgccaggt cgtcacgttc aggaaggacc cgaaagaccc ctcgctgacc ccgaaggagt
     2401 tggcgcgcag caccggcgta ctggcggtag gcgatcgtga gttggtgctg cacagtgacg
     2461 acgccggggc tccagccgtg agcgctagag ttgaaaagct cctgcttgac gtctttgacc
     2521 cgggggagtt gtcggcgatt cgggccgcag tgggtcttgt ctggcgctgc tcagtctgga
     2581 ttccaagagg gagactgctc cctgtcatcg ccccgtacgc ccatagacgc gatggccgcc
     2641 catgcaccgc agcctttcga gacgtcccgg ccgacgagcg agaccggaag tgggagctct
     2701 cgtttgtgac cagagagcct gccgtcgatg agttcctgaa ggcggacgcg gggattgcca
     2761 gaggtgtgag gaagtgaccc ggggcctccg cccgctcctc gcggcggtcc tgttcacgac
     2821 agcgatcggc gcgcgcggcg aagaaccgac gccctggtcc ggcccggtcc ggacggggac
     2881 gatcctggcg ggagacgtcg cgctcttctc gtacgagctg cgcgagaccg acccgctgaa
     2941 gctgcccctc gtgagggagc tggtcgtctg tcggccgcgc ggagagctgt atcggctccg
     3001 ggtcacgagc cggaaggggg acttcgcgct cgagctcggc taccactcgc gagtgaagcc
     3061 ggtcgagccg gaggtcttca tcgggccggt gccgacgaag gacgccccga tcccccgggt
     3121 cccggaggac ccgactggac tctttacggt gccgctgacg aagctccgtg ccgggagcga
     3181 ggagctcgag ttcttcgtcc cgatggactt cttcgtcccg acggtccagc gatggctcga
     3241 ggagacgtgg aagggcacgt cgcgggacct gcctcaggcg ctgaacgacg tcctcgtggc
     3301 ctcgaggctc gtgccgttcg ggatgggcga ggagctgacc tcgctcctcg gagtcgcccc
     3361 caccgccgag ctgagccagg cgctgatcga cgtcctcgcg cgcccgaaat ccgaggcttc
     3421 cgggaaggtc gagggccgga caatgaaatc gttcttccag cttctcccga aggccgagct
     3481 gaaggactac gcggagggcc ggaagctccg ccgcgtggag cgaagccccg agtactgctc
     3541 cgggccgtaa cccgttccgc tccccgcgtc aggcccggcg gccggatgcg cgggagcgga
     3601 ggcgcctcgc gaccgcctcg gccttcgtcc tcagctcctc cagcgtcccg tcggtgacga
     3661 tcacctcgtc gcaggcggcg ttccgctccg cgtcggggag ctgggcgcgc atccgggcga
     3721 ggacggcgtc acgggtggcg cccgggtccc gcgagagggc gcgggcgagg cggagctcct
     3781 cgggggccga ggcggcgacg aggacgtcgt accggtcgcg gccatggttc tcgacgagga
     3841 gcgtcgcctc gacgacggcg accgggtggc cctcggcctc gcgctccgcg agccagcggt
     3901 cggtctcgac gcggacgatc gggtgaatcc gggcgttcag ccgcgcgacc gtggccggct
     3961 cggcgaaggc gaggcgggcg aggcggggcc gatcgacggc gccgcgttcg tcgaggagct
     4021 ccgccccgaa ctcctccgcg accgcgacgg cgcccggcgc gccgggctcg tagagggcgt
     4081 ggacgagccg gtcggcgtcg aggacgggga tgccgagggc gcgccagcgc gacccgacgg
     4141 cgctcttgcc ggaggcgatc ccgccggtga ggccgacgcg cagcatgctc agagccggcg
     4201 ccgggcgcgg cagaggtcga cggtgttcgc gaggaggcgg gcgatcgtga gcggcccgac
     4261 gccgccgggg acgggggtgt agcgcgaggc gcgctcggcc acgtcgaccg ggtggacgtc
     4321 gccgacga
//
LOCUS       DAOONL010000219         4312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_24582_length_4312_cov_16.443035, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000219 DAOONL010000000
VERSION     DAOONL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24582_length_4312_cov_16.443035"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLP50_17025"
     CDS             complement(<1..263)
                     /locus_tag="PLP50_17025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPA53297.1"
                     /translation="MSDATFALVATGAKILFVWVIALATILPNLVWAERRVAGMIQDR
                     PGPNRVGPFGFFQIIADGTKFFMKESVTPLYVDKLLYNLAPWIT"
     gene            complement(260..1972)
                     /locus_tag="PLP50_17030"
     CDS             complement(260..1972)
                     /locus_tag="PLP50_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPA53298.1"
                     /translation="MAKVTVDGRTVEVPDSTNVLEACRAAGVDVPHFCYHPRLSVVGQ
                     CRMCMVEIEGVPKIQASCTVPVRDGMVVLASSEKALAARNATMEFLLINHPLDCPICD
                     QAGECKLQDNAVAAGLPVSRTAEPRRQFPGYDRTSIGPHVIADMTRCIQCTRCIRFCQ
                     EIAGTGELTFIERGGHTIVWTHEGKELANEWSGCTADVCPVGALTTKEFRFRKRVWWL
                     DKARSVCDGCEIGCNVSIEHRDRTVYRFLPRVNLAVNDFWMCDYGRFRAEELNGNDFT
                     RPVLRSAGSARETNWGEALDAVKAAVDAAVAKDPASVLVLGSARLATEESWLLAQLFK
                     GTVGAPQVEFHPDLGPERKIKNPTVPGGWLVGKEAAPNSRGAEAAGLVRAGKGSALAR
                     LLDGSYTPEVLYVADAELTSRVSDPEFAKALRRAKTLIVHGRRTNPLTDAADVLLPVA
                     SLAGKEGTFMNVLGLVQRFQIAIVPPPIVRTDLEVLLHLGKRWGVFDTKWTARDVFER
                     MRESVPGYAGLEWDAPALVGTEPPVSPVSAYDALLERRLQNPAEVLALAAEAAGAAPA
                     GGEK"
     gene            complement(1972..>2811)
                     /locus_tag="PLP50_17035"
     CDS             complement(1972..>2811)
                     /locus_tag="PLP50_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPA53299.1"
                     /translation="GAYICGEETALIESLEGKRGHPRIKPPFPAVSGVWAAPTVVNNV
                     ETLCCVKHIVDRGADWFKGIGRNDRNTGPKLYCVSGHVNRPGVYEAPMGILFKDLLAM
                     CGGVREGRKVKAVIPGGSSAPMLTGEEIMDVPMDFDGVAAAKSMLGSAAVIVMDETTD
                     IPKAVANIAKFYAHESCGQCTPCREGCPWLLKMLKRIVNGQGKKADLDVLLQVAGQMG
                     NGMTICVFADAAIAPVLSAVPRFRAEFEAYVARSAAPGFEVPAPAEITPKLAERMAEG
                     MHS"
     assembly_gap    2812..2911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2912..3197)
                     /locus_tag="PLP50_17040"
     CDS             complement(<2912..3197)
                     /locus_tag="PLP50_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPA53300.1"
                     /translation="MDTLPGQPPVGEHGALSCGQELAFSAKAEERLQKLLTRYPTKQA
                     ALLPTLWIAQEEWGWISREAVDYVAARLGLTSAFVWGVVTFYTMYNRAPVG"
     gene            complement(3218..3580)
                     /locus_tag="PLP50_17045"
     CDS             complement(3218..3580)
                     /locus_tag="PLP50_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPA53301.1"
                     /translation="MGTPDAFLPILLMFTAAAILGAALLFLNGVMGRKVRTLRKLGPY
                     ESGMPLLDDSRKRISVKFFIVAMVFILFDVETGFLWPWATVLDEGGWLVFWPMILFLA
                     VLALADAYLWKKGAFDWE"
     gene            3590..4312
                     /locus_tag="PLP50_17050"
     CDS             3590..4312
                     /locus_tag="PLP50_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA53302.1"
                     /translation="MLIFTKLRGSSTTESPFRPAALVPAFRCETWIGDVVRGLLRHVP
                     EVLVVDDGSEDLTSEAARGAGAKVHRRDANGGKGRAIRDGIELLLAGEWTHVAFVDGD
                     GQHDPDDLPALLDAARDGADFVIGSRLADPEGMPAKNRRANETGDKVLSRMTGLPVED
                     GQSGYRVLSTELLRRLRLTANRYAIENEMLIKAAPILRRFAVVPVRSIYSTGHRHYRP
                     FRDTWVTSWLSVHYKTTGSDVS"
BASE COUNT          627 a         1450 c         1507 g          628 t
ORIGIN      
        1 gtgatccagg gcgcgaggtt gtagaggagc ttgtcgacgt agagcggcgt cacgctctcc
       61 ttcatgaaga acttcgtgcc gtccgcgatg atctggaaga agccgaaggg gccgacccgg
      121 ttcgggccgg ggcggtcctg gatcatcccg gcgacccgcc gctccgccca gacgaggttc
      181 gggaggatcg tggcgagcgc gatgacccag acgaagagga tcttcgcgcc ggtggcgacg
      241 agggcgaagg tggcgtcgct cacttctccc ctccggccgg ggccgctccg gcggcttcgg
      301 ctgcgagcgc gaggacctcg gccgggttct ggaggcggcg ctccagaagc gcgtcgtagg
      361 cggagacagg ggagacggga ggctcggtcc cgacgagcgc gggcgcgtcc cactcgagtc
      421 cggcatagcc ggggacggac tcgcgcatcc tctcgaaaac gtccctcgcc gtccacttcg
      481 tgtcgaagac gccccaccgc ttgccgaggt ggaggaggac ctcgaggtcg gtcctcacga
      541 tcggcggggg gacgatcgcg atctggaacc gctggacgag gccgaggacg ttcatgaacg
      601 tcccctcctt gccggcgagg ctcgcaaccg ggaggaggac gtcggccgcg tcggtgagcg
      661 ggttcgtccg ccggccgtgg acgatcagcg tcttcgcgcg gcggagcgcc ttcgcgaact
      721 cggggtcgga gacccgcgag gtgagctccg cgtcggcgac gtagaggacc tcgggcgtgt
      781 acgagccgtc gaggaggcgg gcgagggccg agcccttccc ggcgcggacg aggcccgcgg
      841 cctcggcgcc gcgcgagttc ggcgcggcct ccttcccgac gagccagccg ccggggaccg
      901 tcgggttctt gatcttccgt tccgggccga ggtcggggtg gaactcgacc tggggggccc
      961 cgaccgtccc cttgaagagc tgtgcgagga gccagctctc ctccgtcgcg agccgggccg
     1021 acccgaggac gaggacggag gccgggtcct tcgcgacggc cgcgtcgacg gcggccttca
     1081 ccgcgtcgag cgcctcgccc cagttcgtct cgcgggcgct cccggcgctc cggaggacgg
     1141 gccgcgtgaa gtcgttcccg ttcagctcct cggcgcggaa gcggccgtag tcgcacatcc
     1201 agaagtcgtt caccgcgagg ttcacgcggg ggaggaagcg gtagaccgtc cggtcgcggt
     1261 gctcgatcga gacgttgcag ccgatctcgc agccgtcgca gacggaccgg gccttgtcga
     1321 gccaccagac gcgcttgcgg aaccggaact ccttcgtcgt gagcgcgccg acggggcaga
     1381 cgtccgccgt gcagcccgac cactcgttcg cgagctcctt gccctcgtgg gtccagacga
     1441 tcgtgtggcc gccccgctcg atgaacgtca gctcgccggt cccggcgatc tcctggcaga
     1501 accggatgca gcgcgtgcac tggatgcagc gcgtcatgtc ggcgatgacg tgggggccga
     1561 tcgacgtccg gtcgtagccg gggaactgcc gccggggctc ggccgtccgg gagacgggga
     1621 gccccgccgc gacggcgttg tcctggagct tgcactcgcc cgcctggtcg cagatcgggc
     1681 agtcgagcgg gtggttgatg aggaggaact ccatcgtcgc gttgcgcgcg gcgagggcct
     1741 tctccgagct cgcgaggacg accatgccgt cgcgcacggg gaccgtgcag gaggcctgga
     1801 tcttcggtac cccctcgatc tcgaccatgc acatccggca ctggccgacg acggagaggc
     1861 gcgggtggta gcagaagtgg gggacgtcga ccccggccgc gcggcacgcc tcgaggacgt
     1921 tcgtgctgtc ggggacctcg acggtccggc cgtcgacggt gaccttcgcc atcaggagtg
     1981 catcccttcc gccatccgct cggcgagctt cggggtgatc tcggccgggg cgggcacctc
     2041 gaacccgggc gccgccgacc tcgcgacgta cgcctcgaac tccgcgcgga accgcgggac
     2101 cgccgagagg accggggcga tcgcggcgtc ggcgaagacg cagatcgtca tcccgttccc
     2161 catctggccg gccacctgga gaaggacgtc gaggtcggcc ttcttcccct gcccgttcac
     2221 gatccgcttc agcatcttca ggagccaggg gcacccctcg cggcagggcg tgcactgccc
     2281 gcatgactcg tgcgcgtaga acttcgcgat gttcgccacg gccttcggga tgtcggtcgt
     2341 ctcgtccatg acgatgacgg ccgccgagcc gagcatcgac ttcgcggcgg cgacgccgtc
     2401 gaagtccatc gggacgtcca tgatctcctc gcccgtcagc atcggggccg aggagccgcc
     2461 cgggatcacg gccttcacct tccgcccttc gcgcaccccg ccgcacatcg cgaggaggtc
     2521 cttgaagagg atccccatcg gcgcctcgta gacgcccggc ctgttcacgt gtcccgacac
     2581 gcagtagagc ttcggcccgg tgttgcggtc gttccggccg atccccttga accagtcggc
     2641 gccgcggtcg acgatgtgct tcacgcagca gagcgtctcg acgttgttga cgacggtcgg
     2701 ggcggcccag acgcccgaga cggcggggaa ggggggcttg atgcgcgggt ggccgcgctt
     2761 gccctcgagg ctctcgatga gcgccgtctc ctcgccgcag atgtaggcgc cnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcccgacgg gggcccggtt gtacatcgtg
     2941 tagaacgtca cgacgcccca gacgaacgcg gaggtcagtc cgaggcgcgc ggcgacgtag
     3001 tcgaccgcct ctcgcgagat ccagccccac tcctcctgcg cgatccagag cgtcgggagg
     3061 agggcggcct gcttcgtcgg ataccgggtc aggagcttct ggagccgctc ctcggccttc
     3121 gccgagaacg ccagctcctg tccgcaggag agggccccgt gctcgcccac ggggggctgg
     3181 ccggggaggg tgtccacggg ggtgctcgcg ccgccgctca ttcccagtcg aacgctccct
     3241 tcttccagag gtaggcgtcc gcgagggcca ggacggcgag gaacaggatc atcggccaga
     3301 agacgagcca cccgccctcg tcgaggacgg tggcccaggg ccagaggaat cccgtctcca
     3361 cgtcgaagag gatgaagacc atcgcgacga tgaagaactt gacggagatc cgcttccggc
     3421 tgtcgtccag gagcggcatg ccgctctcgt acgggccgag cttgcggagg gtccggacct
     3481 tgcggcccat caccccgttc aggaacagga gggcggcccc gaggatcgcg gccgccgtga
     3541 acatgaggag gatggggagg aaggcgtctg gtgtgcccat gaggcgctcg tgctaatttt
     3601 cacaaagttg cggggatcgt caaccacgga aagcccgttc cggcccgccg cgctcgtccc
     3661 cgcgtttcgg tgcgaaacgt ggatcggcga cgtggtacga ggcctcctcc ggcacgtgcc
     3721 ggaagtgctc gtcgtcgacg acgggagcga ggatctcacg tccgaggccg cccgtggggc
     3781 cggagcgaaa gttcaccgcc gcgacgcgaa cggtggtaag gggcgggcga tacgcgacgg
     3841 catcgagctc ctcctcgccg gcgagtggac ccacgtcgcc ttcgtcgacg gcgacggcca
     3901 gcacgacccc gacgacctcc cggccctcct cgacgcggcg cgcgacggag ccgacttcgt
     3961 gatcggctct cgcctcgcgg acccggaggg gatgccggcg aagaaccgcc gcgcgaacga
     4021 gacgggcgac aaggtcctct cgcggatgac cgggctcccg gtcgaggacg gccagtcggg
     4081 ctaccgggtc ctctcgacgg agctcctgcg ccgcctccgc ctcaccgcga accgatacgc
     4141 catcgagaac gagatgctca tcaaggccgc cccgatcctc cggaggttcg ccgtcgtgcc
     4201 ggtccggtcg atctactcga ccggccacag gcactaccgg ccgttccggg acacctgggt
     4261 cacttcctgg ctctcggtcc actacaagac gaccggctcc gacgtctcct ga
//
LOCUS       DAOONL010000220         4283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_24805_length_4283_cov_23.262772, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000220 DAOONL010000000
VERSION     DAOONL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24805_length_4283_cov_23.262772"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLP50_17055"
     CDS             complement(<1..1142)
                     /locus_tag="PLP50_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53303.1"
                     /translation="MRARARFASKCIVFAFLATASPAATAQESGGLATGDGRFRLKGE
                     VKVHFRHSKDYYVTVGRIGNVDIDQRTTDPGGSFEVSTVNLIGEGDLTDGLKAKVEIH
                     FIDLYNRNPTSTGDEVRVREAWLRFGKRVDGLKAFEGTSLYVQAGKAPRFAKQTTRRL
                     ESYGLWGTAVNRFEEIGVEAGGSLGKHLYWRAAAANGNPLFLRDTNSLAGDNGTPERV
                     DLARLGEAPYNSGFPVFYDAQANDTRLSGKAQGGGGLGVRFVSDDGRKGIDLLGWVFH
                     RKLADHVDIEGSLYGGDLDLLDDGLAGRVDLLDGRDKTEAGVNVEARWDRLSLFGQYV
                     RQEVAGLVRKGYEVEASYRIPLNGLFAVGDQPWFNWVQPAVRVSRI"
     gene            1285..1839
                     /locus_tag="PLP50_17060"
     CDS             1285..1839
                     /locus_tag="PLP50_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPA53304.1"
                     /translation="MTQPVVVRKYENRRLYDTSSSRYVNLPEIAQLIREGTEVQVVDA
                     KSGDDITAVILTQIIHEDARAKRGDLPIPFLRELVVASDRAFRDFVVWYADAALSAQR
                     GAKEAFPPLAHVLPFLEKTLEGLKVKAAPAAKPEPADDVAALKERIAELEERLGAARP
                     PRRKAPAAASAPRRGGRRATPRRG"
     gene            complement(1820..3994)
                     /locus_tag="PLP50_17065"
     CDS             complement(1820..3994)
                     /locus_tag="PLP50_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA53305.1"
                     /translation="MRTATESRRSDDLPTVVAPREGSSGASGPPLRGAAAPKPGGVRL
                     GTQFFLAAALLVVVTLGAAVALSTWRANRIAEESIREALRTIPGDVAVFRAGLESQLK
                     ATLRSVAEEPGIKAIFDSPAATVWDTAKDKAEILQAKTFFFFDRAGVLVARSDQPPTE
                     EDRRSFREVAWVASPLEDWTESSATIREGAALAVVAAAPVVAGDPEKGEARLVGVVAA
                     SVLLDEARARELRDLTGGQVAFVAETGRKGEPAKLEIAAATEGFGAASLLPALARDPS
                     GHSRLLDGNADAAPLDLLVGGDRRVVKAVPLRSAGGETLGAVVVSRSRDEETAAFRKI
                     RETLLLVGLGILLVSVPVSFLLGGRIARPLQQLASGAIAVRDGNLDVKLPSGGSGEVG
                     VLARAFEALVGELREKRQLEELLAEMRRRPAEATRGLAPTSVTAAGTLPADAAHAAAR
                     GPRVGALFAGRYDVVSVLGRGGMGSVYQVLDRELDEEVALKVLTPEAFSEGTQATQTL
                     KQEIRLARKITHPNVVRTHDLGEADGVRFLTMEYVPGTTLRELLDRRGAVALAPGLQI
                     AKQLCRGLTAVHEAGIIHRDIKPQNIMVLPNGVVKLMDFGIARTDEGGDGALDGHTVG
                     TPYYMSPEQALGQRLDARSDLYTVGVVLFELFTGERPFPGTDAATVMQSHVAAQPPRP
                     SSLRPDLPEYLDRIVLACLSKRPERRPASASDLYAALTRVAA"
     gene            complement(3978..>4283)
                     /locus_tag="PLP50_17070"
     CDS             complement(3978..>4283)
                     /locus_tag="PLP50_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53306.1"
                     /translation="VVRVYCNIHPQMAAYVVVLDGELFAQSGADGVATIAAVPAGRHA
                     VKVWDERGGEWSGTASVAAGGTTDVSVTLDATKWKETPHKNKHGKDYPPPDDDENRY"
BASE COUNT          602 a         1561 c         1512 g          608 t
ORIGIN      
        1 tcgatccgcg agacgcggac ggccggctgg acccagttga accacggctg gtcgccgacg
       61 gcgaagagac cgttcagcgg gatgcggtag gacgcctcga cctcgtagcc cttcctcacg
      121 agcccggcga cctcctggcg cacgtactgg ccgaagagcg agaggcgatc ccaccgggcc
      181 tcgacgttca cgcccgcctc ggtcttgtcg cgcccgtcga gcaggtccac cctccccgcc
      241 agtccgtcgt cgagaaggtc gaggtcgccg ccgtagaggc tcccttcgat gtccacgtgg
      301 tccgcgagct tccggtggaa gacccagccg aggaggtcga ttcccttccg tccgtcgtcc
      361 gacacgaagc ggacgccgag cccgcctccg ccctgcgcct tgccggagag gcgtgtgtcg
      421 ttcgcctgcg cgtcatagaa gaccgggaat cccgagttgt agggcgcctc gccgaggcgc
      481 gcgagatcca cccgttccgg cgtcccgttg tcgccggcga gcgagttcgt gtcccgcagg
      541 aagagcgggt tcccgttcgc cgccgccgcc ctccagtaga ggtgcttgcc gaggctcccg
      601 cccgcctcga cgccgatctc ctcgaaacgg ttgacggcgg tcccccagag accgtagctc
      661 tcgagtcggc gggtcgtctg cttggcgaag cggggggcct tccccgcctg cacgtagagg
      721 gacgtccctt cgaacgcctt cagcccgtcg acacgcttcc cgaaccggag ccacgcctct
      781 cgcacgcgca cctcatcgcc cgtcgacgtc gggttgcggt tgtagaggtc gatgaagtgg
      841 atctcgacct tcgccttcag gccgtcggtc aggtcccctt cgccgatcag gttcaccgtc
      901 gagacctcga acgagccgcc gggatcggtc gttcgctggt cgatgtcgac gttcccgatc
      961 cggccgaccg tcacgtagta gtccttcgaa tggcggaagt ggaccttgac ctcgcccttc
     1021 agccggaatc ggccgtcgcc ggtcgcgagg ccgcccgatt cctgggcggt cgccgcaggc
     1081 gacgcggtcg caagaaacgc gaagacgatg cacttcgacg cgaagcgagc ccgagcgcgc
     1141 atgggaggcc tccttccgac ggtggccgga tcctaccaag gctcgcgccc gcggcgcgag
     1201 gcggccgcgg agcgcggccc ccgcccgacg gcgaccccgc acctctcctc ccctacaatc
     1261 cccgcacaac ccggaggaag cgtcatgacc cagcccgtcg tggtccggaa gtacgagaac
     1321 cgccgtctct acgacacctc gtcgagccgc tacgtcaacc tgcccgagat cgcgcagctg
     1381 atccgggaag ggaccgaggt ccaggtcgtc gacgcgaaga gcggcgacga catcacggcg
     1441 gtgatcctga cgcagatcat ccacgaggac gcgcgggcga agcggggcga cctcccgatc
     1501 ccgttcctgc gcgagctcgt cgtcgcctcc gaccgcgcct tccgcgactt cgtcgtctgg
     1561 tacgccgacg ccgcgctctc ggcgcagcgc ggcgcgaagg aggcgtttcc cccgctcgcg
     1621 cacgtcctgc cgttcctcga gaagacgctc gaagggctga aggtgaaggc ggctccggcg
     1681 gcgaagccgg agccagcgga cgacgtcgcg gccctgaagg agaggatcgc cgagctcgag
     1741 gagcggctcg gggcggcgcg tcccccgcga cgcaaggcgc ccgcggcggc gagcgcgccc
     1801 cgccggggcg gaaggcgcgc tacgccgcga cgcgggtgag ggccgcgtag aggtcggagg
     1861 cgctcgccgg gcgtctctcg ggtcgcttgg acagacacgc gaggacgatc cggtcgaggt
     1921 attccggcag atcgggccgg agcgaggagg ggcgcggagg ctgcgccgcg acgtggctct
     1981 gcatgacggt cgccgcgtcg gtccccggga aggggcgctc cccggtgaag agctcgaaga
     2041 ggacgacgcc gacggtgtag aggtcgctgc gcgcgtcgag ccgctggccc agcgcctgct
     2101 ccgggctcat gtagtacggc gtgccgacgg tgtggccgtc gagggcgccg tccccgccct
     2161 cgtcggtccg ggcgatcccg aagtccatca gcttcacgac gccgttcggc aggaccatga
     2221 tgttctgagg cttgatgtcg cggtggatga tccccgcctc gtgcacggcc gtgaggccgc
     2281 ggcagagctg cttcgcgatc tggagccccg gggcgagggc gacggccccc cgccggtcga
     2341 gcagctcgcg cagcgtcgtc cccgggacgt actccatcgt caggaaacgg acgccgtccg
     2401 cctcgccgag gtcgtgcgtg cggacgacgt tcgggtgcgt gatcttccgc gcgaggcgga
     2461 tctcctgctt gagcgtctgt gtggcctggg tcccctcgga gaacgcctcc ggggtgagga
     2521 cttttagcgc cacctcttcg tccagctcgc gatcgagaac ctggtagacc gagcccatgc
     2581 cgccgcggcc gaggacggag acgacgtcgt agcgcccggc gaagagcgct ccgacgcggg
     2641 gccctcgcgc cgccgcatgg gcggcgtccg cgggaagcgt cccggccgcg gtcaccgagg
     2701 tcggggccag gcctcgcgtc gcttcggccg gccgcctccg catctcggcg aggagctcct
     2761 cgagctgccg cttctcgcgc agctccccga cgagggcctc gaacgcgcgg gcgaggacgc
     2821 cgacctctcc gctccctccc gacggcagct tcacgtcgag gttcccgtcg cggacggcga
     2881 tcgcgcccga ggcgagctgc tgcagaggcc gcgcgatccg gccgccgagg aggaaactga
     2941 ccgggaccga gacgagcaga atgccgaggc cgacgaggag gagcgtctcg cggatcttcc
     3001 ggaacgcggc cgtctcctcg tcgcgcgagc gcgagacgac gacggcgccg agcgtctctc
     3061 cccctgccga gcggagaggg accgccttga cgacgcgccg gtcgcctccg acgaggaggt
     3121 cgagcggcgc ggcgtcggca ttcccgtcca gcagccggct gtggcccgac gggtcgcggg
     3181 cgagcgccgg aaggaggctc gcggcgccga acccctccgt cgcagccgcg atctccagct
     3241 tcgccggctc gcccttcctc cccgtctcgg cgacgaaggc gacctgacct cccgtcaagt
     3301 cgcggagctc gcgtgcccgc gcctcgtcca ggaggaccga cgcggcgacg acgccgacga
     3361 ggcgggcctc ccccttctcc gggtcgccgg ccacgaccgg ggccgccgcc acgaccgcga
     3421 gcgctgctcc ctcgcggatc gtcgcggagg actcggtcca atcctcgagc ggcgaggcga
     3481 cccaggcgac ctcgcggaac gagcgccggt cctcctcggt cggcggctgg tcgctccggg
     3541 cgacgaggac gccggcgcgg tcgaagaaga agaaggtctt cgcctggagg atctcggcct
     3601 tgtccttcgc ggtgtcccag acggtggcgg ccggagagtc gaagatcgcc ttgatgccgg
     3661 gctcctccgc cacagaccgg agcgtcgcct tcagctgaga ctcgagaccg gcccggaaga
     3721 cggcgacgtc tcccgggatg gtccgaagcg cttcgcggat gctctcctcg gcgatccggt
     3781 tcgcgcgcca ggtggagagc gccaccgcgg ctccgagggt cacgacgacg aggagcgccg
     3841 cggcgaggaa gaactgcgtg ccgagtcgga cccctcccgg tttcggcgcc gccgcgccgc
     3901 gaagaggcgg ccccgacgcc ccggaggagc cctcgcgcgg tgcgaccacc gtcgggaggt
     3961 cgtcggagcg gcgggattca gtagcggttc tcatcgtcgt ccggaggcgg atagtccttc
     4021 ccgtgcttgt tcttgtgcgg cgtctccttc cacttcgtgg cgtccagcgt cacggagacg
     4081 tccgtcgtcc cgcccgccgc caccgaagcg gtcccgctcc actccccgcc tctttcgtcc
     4141 cagaccttca cggcgtgccg gcccgccggg accgccgcga tcgtcgccac gccgtccgcc
     4201 ccggactgcg cgaagagctc gccgtcgagg acgacgacgt aggccgccat ctgcgggtgg
     4261 atgttgcagt agacgcggac gac
//
LOCUS       DAOONL010000221         4272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_24892_length_4272_cov_18.220299, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000221 DAOONL010000000
VERSION     DAOONL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24892_length_4272_cov_18.220299"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2828
                     /locus_tag="PLP50_17075"
     CDS             <1..2828
                     /locus_tag="PLP50_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPA53307.1"
                     /translation="NLGAKEGLPSSCVFSMSETPDGDLWLGTMRGAARLLRGAEPRVV
                     EVVGEAQGLANELVFRAERDPDGSLWVWSEGGLQRRRPGEERLGPVDEYGSAISADVW
                     GFAFDGDARLWVGSRRAEGGLAVRRADGTWRVFDRLPDGSRIDVAHALARRRAGGVWA
                     AFGARLAACDGETVVPLPDPPAAMSSPYVNKLFEDGKGRLWAASGSGLARLETDGTWT
                     LLTDRLGSPYVYFVGEDRTGTIWVGTTRGVYRLGAGDAVTPFTPDDGLAGWETNINAF
                     HTDRDGVVWIGTVDGLSRYDPASHRPNAHPPRVVVESVRLPGREVSFPDRLELSWSER
                     SAAFRVAVLSYRARGRTAYRARLEGLEEGWLPPRRESELRYTNLPAGDLTLHVQGINE
                     SGLWGEVVKIPIRVHPPFWMAAWFRAAVLLALVAAVAATFRWRTLVLRRRNRELERVV
                     AERTTDLANANDHLRHAQGEIARLLEASPAPSENVASWSQALAADVASAIGAERIGIW
                     EVRDDDVVPLSDAGLAPPRIDDLRGSTPGSPRPLPSEEGAAVVPVSGMSSELRGALRV
                     LGPAVVWGETERRLVGGFAHQLGAALDMAHMRRQLSTLREKREATLREMHEQGIATLQ
                     ICPLCGRCYDQAAEECVVDGSPLDTPRPLPFELLGRYRFERILGQGGMATVFSARDTR
                     LARDVAIKLIRPEHFGDSELKARFEHEARTIARISHPGVIGLFDSGELPDGTAFLVME
                     MLRGHDLAHVLLECGTGTPRQVAQLAREAGAALAAAHAAGVVHRDIKPGNLFLADDPA
                     GFRVKVLDFGLAKEMAHEHGLTRTGMVVGTPEYMSPEQVHGWNVDARTDVYSLGAVCY
                     EALLGRRAIRGEDLGRIMANVIDEVPPPASSVLPGVPEAASVALAAALAKDVFERPSD
                     VAAWSETLAASLETMPDGLASGWTRID"
     gene            2863..3666
                     /locus_tag="PLP50_17080"
     CDS             2863..3666
                     /locus_tag="PLP50_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA53308.1"
                     /translation="MRRDRLLPVAALALAAIVPVLPATAQEENRATDAQRLRRYKGAI
                     AKYERALKLLEKNDRERALDALAVSIEEMPDFPDSHLLRARLLYGEKEYSRALEEVVA
                     ARAAYEKTADLRAGMQADRRKALTERLRAKDAAISEQSGRLAKAPADQRAQIERLVAR
                     LQGERFDIERELHEHRPDPQGVPARYSSLHGNVLLRLGRDAEAIAQYEEALKADPAWG
                     EAANNLASLYHMAGKHERALEIVTQAEKLGAPLHPELKKSIEAALARRP"
     gene            3793..>4272
                     /locus_tag="PLP50_17085"
     CDS             3793..>4272
                     /locus_tag="PLP50_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPA53309.1"
                     /translation="MGTRGREIVGIDVEKVIEMLNAAYASEWLAYYQYWLGAKLIKGP
                     MKDAVAAELNLHATEELNHAVMVAGRILQLGGTPLLDPHEWFDKSPCAYDAPKDPYVA
                     VLLDENIAGEQCAIDTYKKIMDATKDKDMVTYNMALTILEQEVEHEEDLQSLKEDLDL
                     "
BASE COUNT          626 a         1572 c         1543 g          531 t
ORIGIN      
        1 ggaacctcgg ggcgaaggag gggctgccgt cctcgtgcgt cttctcgatg agcgagacgc
       61 cggacggaga cctctggctc gggacgatgc gtggagcggc tcgcctcctg cgcggcgcgg
      121 aaccgcgcgt cgtcgaggtc gtgggcgagg cgcaggggct cgcgaacgag ctcgtcttcc
      181 gcgccgagcg cgaccccgac gggagcctct gggtctggtc ggaagggggc ctgcagcgcc
      241 ggcggccggg cgaggagcgg ctcgggccgg tcgacgaata cggctcggcg atcagcgccg
      301 acgtctgggg gttcgccttc gacggcgacg cgcgcctctg ggtcggcagc cgccgcgccg
      361 agggcgggct cgccgtgcgt cgcgcggacg ggacgtggcg cgtcttcgac cgcctcccgg
      421 acggctcgcg gatcgacgtg gcgcacgccc tggcgcggcg ccgcgcgggg ggcgtctggg
      481 cggcgttcgg cgcacgcctc gccgcgtgcg acggcgagac tgtcgtcccg ctccccgacc
      541 cgcccgcggc gatgagcagc ccctacgtga acaagctctt cgaggacggc aaggggcgcc
      601 tctgggccgc gagcgggagc ggcctggccc ggctcgagac ggacgggacg tggacgctcc
      661 tgacggaccg cctcggcagc ccgtacgtct acttcgtcgg cgaggaccgg acggggacga
      721 tctgggtcgg gacgacgcgg ggcgtctaca ggctcggcgc gggcgacgcc gtgacgccgt
      781 tcacgcccga tgacggcctc gccgggtggg agacgaacat caacgcgttc cacaccgacc
      841 gcgacggcgt cgtctggatc gggacggtcg acggcctctc gcggtacgac ccggcctcgc
      901 accgcccgaa cgcccacccg ccgcgcgtcg tcgtcgagtc ggtccgcctc ccggggcggg
      961 aggtctcgtt ccccgaccgc ctcgagctct cctggtcgga gcggtcggcg gccttccgcg
     1021 tggcggtcct ctcgtaccgc gcccggggac ggacggccta ccgcgcccgc ctggaggggc
     1081 tcgaggaggg ctggctcccg ccgcgacgcg agtccgagct tcgctacacg aacctccccg
     1141 ccggcgacct gacgctccac gtccagggga tcaacgagtc ggggctctgg ggcgaggtcg
     1201 tgaagatccc gatccgagtc cacccgccgt tctggatggc ggcgtggttc cgcgccgccg
     1261 tcctcctcgc gctcgtcgcc gcggtggccg ccacgttccg ctggaggacg ctcgtcctcc
     1321 gccggaggaa tcgggagctc gagcgggtcg tcgcggagcg gacgaccgac ctcgccaacg
     1381 cgaacgacca ccttcggcac gcccagggcg agatcgcgcg gctcctcgag gcgagcccgg
     1441 ccccgtccga gaacgtggcg agctggtccc aggcgctcgc cgccgacgtc gcgagcgcga
     1501 tcggcgcgga gcggatcggc atctgggaag tgcgggacga cgacgtcgtc cccctctccg
     1561 acgccggcct cgccccgccg cgaatcgacg acctccgcgg ctcgaccccg ggcagcccgc
     1621 gcccgctccc gtcggaggag ggagcggccg tcgtgccggt ctccggaatg agctccgagc
     1681 tccgcggcgc ccttcgcgtc ctgggtccgg cggtcgtctg gggagagacc gagcggcggc
     1741 tcgtgggcgg gttcgcgcac cagctcggcg ccgcgctcga catggcccac atgcgccgcc
     1801 agctctcgac cctccgcgag aagcgagagg cgacgctccg cgagatgcac gagcagggga
     1861 tcgccacgct ccagatctgc ccgctctgcg ggcgctgcta cgaccaggcg gccgaggaat
     1921 gcgtggtcga cggctccccg ctcgacacgc cgcgaccgct ccccttcgag ctgctcggcc
     1981 gctaccggtt cgagcgcatc ctcgggcagg gggggatggc gacggtcttc tccgcccgcg
     2041 acacgcgcct cgcgcgcgac gtcgcgatca agctgatccg gccggagcac ttcggagatt
     2101 ccgagctcaa ggcccgtttc gagcacgaag cgcgaacgat cgcccggatc tcgcatcccg
     2161 gcgtcatcgg cctcttcgac tccggcgagc tccccgacgg caccgcgttc ctcgtgatgg
     2221 agatgctccg cgggcacgac ctggcgcacg tcctcctcga gtgcgggacc gggactccgc
     2281 ggcaggtcgc ccagctcgcg cgcgaggcgg gcgcggcgct cgccgccgcc cacgcggcgg
     2341 gcgtcgtcca ccgcgacatc aagccgggga acctcttcct cgccgacgac ccggcgggct
     2401 tccgcgtgaa ggtgctcgac ttcggcctcg ccaaggagat ggcccacgag catggtctga
     2461 cgcgcacggg aatggtcgtc gggacgccgg agtacatgtc ccccgagcag gtgcacggct
     2521 ggaacgtcga cgcgcgcacc gacgtctact ccctcggcgc cgtctgctac gaggcgctcc
     2581 tcggccgccg ggcgatccgg ggcgaggacc tcgggcggat catggcgaac gtcatcgacg
     2641 aggtcccgcc ccccgcctcg tcggtcctcc ccggagtccc ggaggcggcc tccgtcgccc
     2701 tcgcggcggc cctcgcgaag gacgtcttcg agaggccgtc cgacgtcgcc gcctggtcgg
     2761 agaccctcgc cgcctcgctc gagacgatgc cggacggcct cgcctcgggc tggacgagaa
     2821 tcgactgaaa cgcgcggcgc ggccgcgccc cggaggatcg gcatgagacg agaccgcctg
     2881 ctccccgtcg ccgcgctcgc cctcgcggcg atcgtccccg tcctcccggc cacggcgcag
     2941 gaggagaacc gcgcgacgga cgcgcagcgg ctccgccgct acaagggcgc gatcgcgaag
     3001 tacgagaggg cgctgaagct cctcgagaag aacgaccgcg agcgggcgct cgacgccctc
     3061 gccgtcagca tcgaggagat gcccgacttt ccggactcgc atctcctccg ggcgcggctc
     3121 ctctacggcg agaaggagta ctcccgcgcg ctcgaggagg tcgtcgccgc ccgggccgcg
     3181 tacgagaaga ccgccgacct gcgcgcgggg atgcaggccg accgccggaa ggcgctgacg
     3241 gagaggttgc gcgcgaagga cgccgcgatc tcggagcagt ccggacgcct cgcgaaggct
     3301 ccggccgacc agcgggcaca gatcgagagg ctcgtcgccc ggctccaggg cgagcggttc
     3361 gacatcgagc gcgagctcca cgagcacaga cccgacccgc agggcgtgcc ggcccgctac
     3421 agcagcctcc acgggaacgt cctcctccgg ctcggccggg acgccgaggc gatcgcgcag
     3481 tacgaggagg cgctgaaggc cgacccggcg tggggcgagg cggcgaacaa cctcgcgagc
     3541 ctctaccaca tggccgggaa gcacgagagg gcgctcgaga tcgtcaccca ggccgagaag
     3601 ctgggtgcgc cgctccaccc cgagctgaag aagtcgatcg aagcggccct cgcccgccgt
     3661 ccgtaggccg gaggcgggcc gggacccggg ccggctcctc cggttccccc gaccctcaat
     3721 cgggcatatg cttacaggcc gcaggttggg acggcccgcc ggccggaccc cgcccgaatc
     3781 gaggaggtaa cgatgggcac cagaggacgc gagatcgtcg gcatcgacgt cgagaaggtc
     3841 atcgagatgc tgaacgcggc ctacgcgagc gagtggctcg cgtactacca gtactggctc
     3901 ggcgcgaagc tgatcaaggg accgatgaag gacgccgtcg ccgcggagct gaacctgcac
     3961 gccaccgagg agctgaacca cgccgtcatg gtcgcgggcc ggatcctcca gctcggcggg
     4021 acgccgctcc tcgacccgca cgagtggttc gacaagagcc cctgcgccta cgacgccccg
     4081 aaggacccgt acgtcgcggt cctcctcgac gagaacatcg ccggcgagca gtgcgccatc
     4141 gacacctaca agaagatcat ggacgccacg aaggacaagg acatggtgac ctacaacatg
     4201 gcgctcacga tcctcgagca ggaggtcgag cacgaggagg acctccagag cctcaaggag
     4261 gacctcgacc tc
//
LOCUS       DAOONL010000222         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_24958_length_4263_cov_23.039924, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000222 DAOONL010000000
VERSION     DAOONL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24958_length_4263_cov_23.039924"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..777
                     /locus_tag="PLP50_17090"
     CDS             316..777
                     /locus_tag="PLP50_17090"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53310.1"
                     /translation="MSSSRSTWGASARTSSSRRASTSTCKEAGSLHLAEEAATKRRAF
                     LLKPDILVRKGRSPRVVADTKWKRLDPRSADLGVSGEDVYQVLAYAHRYATDTAVLIY
                     PHHATVGRPGLQRDFAVQAGGGSRVVVRIATLDLARLERVVEEVGEAVRAG"
     gene            867..1466
                     /locus_tag="PLP50_17095"
     CDS             867..1466
                     /locus_tag="PLP50_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPA53311.1"
                     /translation="MGNETRNNRKQPDWDGLFAIAEAQSGYFTTSQAAQSGYSRPLLH
                     KYLATGRMTRVRRGVYRLVHFPVSEHEDLVVLWLWTGQVGVFSHETALALHDLSDALP
                     GKVHVTVPSNWLRRRLRVPAGLVLHFADLGDGDRSSYSAVPVTAPLRTLRDCIEAEVA
                     ADLVRQGILQARRRGIVSAKDEARLLAELGRPGRTVAAR"
     gene            1463..2368
                     /locus_tag="PLP50_17100"
     CDS             1463..2368
                     /locus_tag="PLP50_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPA53312.1"
                     /translation="MTARQYSTPLAFKQALEQRLRTSAATGVDFARRRQLLIFDRFLG
                     RLAQVAGDAVVLKGGLVLELRLARARTTKDIDLRMMGSSDEVLELLQEAGRLDLGDYM
                     LFEVQPDADHPEIQNDGMRYDGFRFRAECRLAGMIYGRPFGVDVAFGDPLVGEPDVVV
                     TDEVLAFAGIAPSSLRLYPVVSHIAEKLHAFTMPRPRPNSRVRDLPDLALLATTGPIE
                     GAALRRAIELTFEFRGTHPAPMKCPPPPMDWAVPYARMAENDDLTWKTLAEVTLAVET
                     FLNPVLESAECGTWTPSGWRWRKGR"
     gene            2698..3162
                     /locus_tag="PLP50_17105"
     CDS             2698..3162
                     /locus_tag="PLP50_17105"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53313.1"
                     /translation="MSSSRSTWGASARTSSSRRASTSTCKEAGSLHLAEEAATKRRAF
                     LLKPDILVRKGRSPRVVADTKWKRLDPRSADLGVSGEDVYQVLAYAHRYATDTAVLIY
                     PHHATVGRPGLQRDFAVQAGGGSRVVVRIATLDFARLERVAGEVGAAVGAVP"
     gene            complement(3165..3740)
                     /locus_tag="PLP50_17110"
     CDS             complement(3165..3740)
                     /locus_tag="PLP50_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53314.1"
                     /translation="MRITAHSTLEQVAAVLAGVLREAGIKAVLTGGACATIYSAGGHQ
                     SEDLDLILQSSPTQRSLDEAMAKAGFVRNGDFYEHPRTSFFVEFPRGPLAIGTDTSIA
                     PVEVQVEGVPVLLLSAEDSCRDRLAAFDHWKDRQALGAAVAIATNHPLDLEKTRAWSR
                     REGAAAGFVEFQRTLEAAKAASHRSRPRGPR"
     gene            complement(3737..4066)
                     /locus_tag="PLP50_17115"
     CDS             complement(3737..4066)
                     /locus_tag="PLP50_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPA53315.1"
                     /translation="MAVTSPFGSRSRTRLLIALELLGQTYPRELARLLGSSLSAAQKG
                     LESLERDGLVAGRLVGRTRVVQLNPAFFAMKEVRALLVRLVEADRELESRIAALRRRP
                     RKSGKRL"
BASE COUNT          640 a         1531 c         1447 g          645 t
ORIGIN      
        1 tccgctgcct cttcgacgag cgctcggccg acacgcccct gaaccggacg ctgaaggcgg
       61 cgctcctcgc cgccgagagc atgctcgagg gcgccgccgc gacgagcgac gccgtcgagc
      121 tgcgacacct cctcgccgac gtctcggacg cgtgcccgcc ccgcgaggtc gtcgcgcggc
      181 tccaaaccga ccggatgagc cgccgcctcg cgcccctcct cgccctggcg aagctcctcc
      241 tcggcgaccg aagccccgac ctcggccgcg ccgcgacggc cgaccggcgg agctacgccg
      301 tggtgtggga catgaatgtc ctcttcgagg agtacgtggg gcgcgtctgc caggacgtct
      361 tcgagccgaa gggcctcgac gtcgacctgc aaggaggccg gctccctcca tctcgccgag
      421 gaagcggcga cgaagcggcg ggccttcctc ctgaagcccg acatcctcgt ccgcaagggc
      481 cgcagccccc gcgtcgtcgc cgacacgaag tggaagcgcc tcgacccgcg aagtgccgac
      541 ctcggcgtct cgggcgagga cgtctaccag gtcctcgcct acgcccaccg ctacgccacc
      601 gacaccgccg tcctcatcta cccccaccac gcaaccgtcg ggcggccagg cctccagcgg
      661 gacttcgctg tccaggccgg gggcggctcg cgggtcgtgg tgaggatcgc gacgctcgat
      721 ttggcgcggc tcgagagggt ggtggaggag gtgggggagg cggtgcgggc cgggtaatcg
      781 ccggcgaacg cccgcgtccc tggcgaggtt gaaaccgagc gtccctgtcc gcacagacgt
      841 taaccgtgag ttaacattcg cgcggcatgg gtaacgagac ccggaataat cggaagcagc
      901 cggattggga tggactcttc gctatcgcag aggcccagag cggctacttc acgacgtctc
      961 aggccgcgca gtcgggctac tcgcgtccac tcctccacaa gtacctggcg acggggagga
     1021 tgacgcgcgt gcggcgcggc gtgtaccggc tcgtccactt cccggtcagc gaacacgaag
     1081 acctcgtcgt tctctggctc tggaccggcc aggtcggggt cttctcccat gagacggccc
     1141 ttgcgctcca cgatctctcc gacgcccttc ccggaaaggt ccacgtgacg gtgccctcga
     1201 actggctgcg gcgtcggctc cgcgtcccgg ccggcctcgt gcttcacttc gccgacctcg
     1261 gcgacggtga tcgctcgagc tactcggcgg tgcccgttac ggcgccactc cgcacccttc
     1321 gagactgcat cgaggccgag gtcgccgccg acctcgtgcg gcaaggaata ctccaggccc
     1381 gtcgacgggg gatcgtctcg gcgaaggatg aagctcgact tcttgccgag ctgggcagac
     1441 caggtcggac agtggccgcg cgatgaccgc tcgccagtac tcgacgcccc tggccttcaa
     1501 gcaggccctc gagcaacggc tccgaacctc ggcggccacc ggcgtcgact tcgcccgtcg
     1561 ccgccagctg ctcatcttcg atcgcttcct cggccgcctg gctcaggtcg cgggcgacgc
     1621 cgtggtgctc aagggcgggc tcgtcctcga gctgcgtctc gcgcgggctc ggacgacgaa
     1681 ggacatcgac ctccgcatga tgggctcgtc cgacgaggtg ctggagctgc tccaggaggc
     1741 aggccgcctc gacctcggcg actacatgct gttcgaggtc cagccggacg ccgatcatcc
     1801 cgagatccag aacgacggga tgcgctacga cggcttccgc tttcgagcgg agtgtcgcct
     1861 cgcgggcatg atctacggcc ggccgttcgg cgtcgacgtc gccttcggcg acccgctcgt
     1921 gggcgagccg gacgtcgtcg tgaccgacga ggtcctcgca ttcgcgggca tcgccccttc
     1981 gtcgctccgg ctctatcccg tcgtctctca catcgccgag aagctccacg cgttcacaat
     2041 gcctcgaccg agaccgaact cacgagtccg ggatctccct gacctggctc tgctggcgac
     2101 gaccggaccg atcgaagggg ccgctctccg tcgggccatc gagctgacgt tcgagtttcg
     2161 cgggacgcac ccggctccaa tgaagtgtcc gccgcctccg atggattggg cggttccgta
     2221 cgcgaggatg gccgagaacg atgacctcac ctggaagact ctcgcggaag tgacgctggc
     2281 tgtggagacc ttcctcaatc ccgttctcga atccgccgaa tgcggaacct ggacgccgtc
     2341 gggctggcgc tggaggaagg gtcgctgaga gaggaaccgg aaacggggag aagggggccg
     2401 cgccctcggc cgacacgccc ctgaaccgga cgctgaaggc ggcgctcctc gccgccgaga
     2461 gcatgctcga gggcgccgcc gcgacgagcg acgccgtcga gctgcgacac ctcctcgccg
     2521 acgtctcgga cgcgtgcccg ccccgcgagg tcgtcgcgcg gctccaaacc gaccggatga
     2581 gccgccgcct cgcgcccctc ctcgccctgg cgaagctcct cctcggcgac cgaagccccg
     2641 acctcggccg cgccgcgacg gccgaccggc ggagctacgc cgtggtgtgg gacatgaatg
     2701 tcctcttcga ggagtacgtg gggcgcgtct gccaggacgt cttcgagccg aagggcctcg
     2761 acgtcgacct gcaaggaggc cggctccctc catctcgccg aggaagcggc gacgaagcgg
     2821 cgggccttcc tcctgaagcc cgacatcctc gtccgcaagg gccgcagccc ccgcgtcgtc
     2881 gccgacacga agtggaagcg cctcgacccg cgaagtgccg acctcggcgt ctcgggcgag
     2941 gacgtctacc aggtcctcgc ctacgcccac cgctacgcca ccgacaccgc cgtcctcatc
     3001 tacccccacc acgcaaccgt cgggcggcca ggcctccagc gggacttcgc tgtccaggcc
     3061 gggggcggct cgcgggtcgt ggtgaggatc gcgacgctcg attttgcgcg gctcgagagg
     3121 gtggcggggg aggtgggggc ggcggtgggg gccgtcccgt gaattcagcg ggggccgcgt
     3181 ggtctggagc gatgcgaggc ggccttcgca gcctccaggg tgcgctggaa ttccacgaac
     3241 cccgccgccg ccccctctcg gcggctccag gcgcgggtct tctcgagatc gagggggtga
     3301 ttcgtggcaa tggcgacggc ggctccaagg gcctggcggt ctttccagtg gtcgaacgcc
     3361 gcgagccgat ccctgcacga gtcttcggcc gataggagca ggaccgggac gccctccacc
     3421 tgcacctcga ccggcgcgat cgaggtatcg gtcccgatcg ccagagggcc ccgagggaac
     3481 tcgacgaaga acgaggttcg cgggtgctcg tagaagtcgc cgttccggac gaaccctgcc
     3541 ttggccatgg cttcgtcgag cgaccgttgc gtcggtgaag actggaggat cagatcgagg
     3601 tcttcggact ggtgcccgcc tgccgagtag atcgtcgcgc acgctccgcc ggtgagcacg
     3661 gccttgatcc cggcttcgcg gagtacgccg gccaggactg ccgcgacctg ctcgagggtg
     3721 gagtgcgccg tgatcctcac agccgcttcc cgctcttccg gggcctgcgg cgaagcgcgg
     3781 cgatcctcga ctccagctcg cgatcggctt cgacgagccg gacgagcagc gcgcggacct
     3841 ccttcatggc gaagaacgcg gggttcagct ggaccacccg ggttcgcccg accaggcggc
     3901 ccgcgacgag gccgtcgcgc tccaggctct cgaggccctt ctgcgccgcc gacagcgaag
     3961 aacccaggag gcgcgccagc tcccgggggt aggtctgccc gagaagctcc agggcgatca
     4021 ggagacgggt gcggctgcgg gagccgaagg gtgatgtgac cgccattagc tgtcatatga
     4081 ccttctttgg cggtcgatgt caagggtacc caactccgag gtgtcctccg cggcaaggtc
     4141 cactggccct cccaggcgaa gcggcaggcc gcgcagcgcc tcgagttccg ctgcctcttc
     4201 gacgagcgct cggccgacac gcccctgaac cggacgctga aggcggcgct cctcgccgcc
     4261 gag
//
LOCUS       DAOONL010000223         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25111_length_4244_cov_17.711864, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000223 DAOONL010000000
VERSION     DAOONL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25111_length_4244_cov_17.711864"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2926)
                     /locus_tag="PLP50_17120"
     CDS             complement(140..2926)
                     /locus_tag="PLP50_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPA53316.1"
                     /translation="MKTRFLLAALLVLAALPAGGEELWGRRIESLAFRGDAPVEEASF
                     ARLTDLGPGQVLTKSAVHSSLRNLFATGRFADLAVEAAPSPDGAVVTIVFAAAARVAE
                     VEVAGERIPVKGRVLDALKTRPRDRWTEERGRTATRAALRVLEERGYFEAEVTPWVSP
                     GPDDTSVNVRFEVVAGRPAAPAPAEFAGDLGPLTADELRRKGKLRKERTFREAVARED
                     AERYAALYHSLGRSRAEVRYEGVSYEKGSGLATARYAVFAGPDVVLDVTGIAEAEVRR
                     HPSSPWAKGDPPDADALERLRTALREGLQRKGYARAEVDVDVEVSSDREEVRLHAERG
                     ERWVVGRTVVEGTCAVPERSLLAVLRTRPRGIFETGRLVDSDLAADRASLLAVYRARG
                     FPDAKVTAEVLGGALPYELDVRFHVDEGPAAWLGRVSIEGLATIDTATVSRRLRVRHG
                     GPYLQEDVDADLASLRSALGDGGFPDGRVEARASRRPAEPGAPIVVDVVYEVSEGPRA
                     VFGKTILRGARATRLSVLERELAYEEGTPFSMARLIRTQQNLDRLGVFSRVDVSGLSP
                     EPGGPARSILVQVEEAKPWSLLYGLGVEYDERAEKAFNPRLSLALTHANLFGRAIAGV
                     VEARSSRRDTRVLVRLADRSVFDSGLAGTITGYVAEEIRQSYSVRRGGAFFEASRVFA
                     GTVKGALRYQYEIVEPDAPDDSILSEIERQDQRIFINSIGASAVVDRRDDPIVPTKGF
                     FAAAEGKWAFPAASADAHFLKGSTQLSLYRPFRGGTLAAGVRAGLTESLVTCNPEANP
                     GCLPNLDTPIVERVFAGGRTTHRSFPLDDLGVPGQTLKDGRGIGGNAFLLANLEWRVP
                     LTGGLGVVLFADWGNVWAGPAYVRLPEGRWGAGLGIHYLTPVGPFRLEYGFRLDRKKD
                     EDTGALSFSIGYPF"
     gene            complement(2923..>4244)
                     /locus_tag="PLP50_17125"
     CDS             complement(2923..>4244)
                     /locus_tag="PLP50_17125"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPA53317.1"
                     /translation="ETGGAGAIDVRAAGTMEAAFLAPFLGVASLEGRMAVSATASGTT
                     ARPVFEGRVVLEDLELVTSPGEEPVEAISGTILLTPGRVTTDDLTLRWNGDVAVAGAA
                     TLDGFAMTGMRLNVHLDGLRGEPFPGLRSTVSGDLVLLGDDELRSARGELTMTRGLYA
                     EDLDLSLQSLLGGRRGAAEAPPEPTRFDDVDLEVRVAIPPGALEVRNNVARLRGSGDL
                     TVRGTFGHPLLLGTIEATEGGRLELRGLRYDVTRAKLVFANPAANDPYFELEARTQVK
                     EYAITLGVTGTASRIVPRFTSYPQLPEAQIVSILATGEIPSTTAGSVGSVSPVSTDQD
                     IVAAARELITGLATDAAASRTKEFLRLDRLQIDPVFIGSTFDAPRLTVAKRLSNDLTV
                     TYSYKASTDQEQVLLVEYQLSPAAFLQLLRDENGVYSAEVKIRQRLR"
BASE COUNT          610 a         1534 c         1543 g          557 t
ORIGIN      
        1 caggcgtgac aggcctgcgt tctaaccaac tgaactacga ccccgtttcg aaagaccggc
       61 ccttacgggc ggacgcgcat tatacgcagg agcctctccg acgcaagcgg gcggagcgtt
      121 cggtcaccgc cgcgccccgt cagaacgggt agccgatgct gaagctgagc gcccccgtgt
      181 cctcgtcctt ctttcgatcg aggcggaagc cgtattcgag ccggaaggga ccgaccggcg
      241 tcaggtagtg gatcccgagg ccggcgcccc agcgcccctc cgggagcctc acgtaggccg
      301 ggccggccca gacgtttccc cagtcggcga agaggacgac cccgagccct cccgtgagcg
      361 gcacccgcca ctccaggttc gccaggagga aggcgttccc tccgatgccc cgcccgtcct
      421 tcagagtctg gcccggcact ccgaggtcgt cgagagggaa cgagcggtgc gtcgtccggc
      481 cgccggcgaa gaccctctcg acgatcggcg tgtcgaggtt cgggagacac cccgggttcg
      541 cctccgggtt acacgtgacg agagactccg tcaggcccgc ccgaacgccc gcggcgagcg
      601 tcccgccgcg gaacggccgg tagagagaga gctgcgtcga gcccttcagg aaatgcgcgt
      661 ccgccgaggc ggccgggaag gcccacttcc cctccgccgc ggcgaagaaa cccttcgtcg
      721 ggacgatcgg gtcgtcccga cggtcgacga cggcgctcgc tccgatggag ttgatgaaga
      781 tccgctggtc ctgccgttcg atctccgaaa ggattgagtc gtccggcgcg tcgggctcca
      841 cgatctcgta ctggtaccgc agcgccccct tgaccgtccc ggcgaagacg cggctcgcct
      901 cgaagaaggc ccccccgcgc cggacgctgt aggactgccg gatctcctcg gcgacgtagc
      961 cggtgatcgt gcccgccagt ccggagtcga agaccgaccg gtcggcgagg cggacgagga
     1021 cccgcgtgtc cctccgcgag gagcgcgcct cgaccacgcc ggcgatcgcc cgtccgaaga
     1081 ggttcgcgtg cgtcagggcg agtgagaggc gcgggttgaa ggccttctcg gcccgctcgt
     1141 cgtactcgac gccgaggccg tagaggaggc tccagggctt cgcctcctcc acctggacga
     1201 ggatcgagcg ggcggggccg cccggctccg gggagaggcc ggagacgtcg acgcgggaga
     1261 agacgccgag ccggtcgagg ttctgctggg tgcggatgag cctcgccatc gagaacggcg
     1321 tcccttcctc gtacgcgagc tcgcgctcga ggacggagag gcgcgtcgcc cgcgctcccc
     1381 ggaggatcgt cttcccgaag accgcccgcg gtccctcgga gacctcgtag acgacgtcga
     1441 cgacgatcgg cgctccgggc tccgcgggtc gccgcgaggc acgcgcctcg acgcgtccgt
     1501 ccgggaagcc gccatccccc agcgccgacc ggagggacgc gaggtccgcg tcgacgtcct
     1561 cctggagata cggaccgccg tgccgcacgc gcaggcgccg tgagaccgtg gcggtgtcga
     1621 tcgtggcgag cccttcgatc gagacgcggc cgagccaggc ggccggtccc tcgtccacgt
     1681 ggaagcggac gtcgagctcg tacgggagcg ccccgccgag gacctcggcg gtcaccttcg
     1741 cgtccgggaa gccgcgggca cggtagaccg cgaggagcga ggcgcgatcg gccgcgagat
     1801 ccgagtcgac gagacgcccc gtctcgaaga tcccccgcgg ccgggtccga agcacggcca
     1861 ggagcgacct ctcggggacc gcgcacgtcc cctcgacgac ggtccgcccc acgacccagc
     1921 gttcgcctcg ctcggcgtga aggcgaacct cctcgcggtc cgaggagacc tcgacgtcga
     1981 cgtcgacctc ggcgcgcgcg tagcccttcc gctggagccc ctcccggagc gccgtgcgga
     2041 gccgctcgag ggcgtcggcg tcgggcgggt cccctttcgc ccagggagac gacggatgcc
     2101 gtcggacctc cgcctcggcg atccccgtca cgtcgaggac gacgtcgggg cccgcgaaga
     2161 cggcgtaacg ggccgtcgcg aggcccgagc ccttctcgta agacacgccc tcgtagcgca
     2221 cttccgctcg ggagcggccc aggctgtggt agagcgccgc gtagcgctcc gcgtcctccc
     2281 tcgcgaccgc ctcccggaac gtccgctcct tccggagctt ccccttccgc cggagctcgt
     2341 cggccgtgag cggtcccagg tcccctgcga actccgccgg cgccggcgcg gccggccggc
     2401 ccgccacgac ctcgaaccgg acgttcaccg aggtgtcgtc gggcccgggc gagacccacg
     2461 gcgtcacctc ggcctcgaaa tagccgcgct cctcgaggac gcgaagcgcc gcccgcgtcg
     2521 ccgtccggcc gcgctcctcg gtccagcggt cgcgcgggcg ggtcttcagg gcgtcgagga
     2581 cccggccctt caccgggatc cgctcccccg ccacctcgac ctcggcgacg cgagccgcgg
     2641 cggcgaagac gatcgtgacg acggcaccgt ccggcgacgg cgccgcctcc acggcgaggt
     2701 cggcgaagcg tccggtggcg aagaggttcc ggagcgacga gtggacggcg ctcttcgtca
     2761 gcacctgccc ggggccgagg tcagtgaggc gcgcgaacga cgcctcctcg accggcgcgt
     2821 cgccccggaa ggcgagcgac tcgatgcgcc ggccccagag ctcctccccg cccgccggga
     2881 gcgcggcgag gacgaggagc gcggcgagga ggaagcgcgt cttcaacgga gcctctgacg
     2941 gatcttcacc tccgccgagt agacgccgtt ctcgtcccgg aggagctgga ggaaggccgc
     3001 cggggagagc tggtactcga cgaggaggac ctgctcctgg tccgtcgagg ccttgtagga
     3061 gtaggtgacc gtcaggtcgt tcgagagacg cttggcaacg gtgagccggg gcgcgtcgaa
     3121 cgtggacccg atgaagacgg ggtcgatctg gaggcggtcg aggcggagga actccttcgt
     3181 ccggctcgcg gcggcgtcgg tggcgagccc cgtgatcagc tcgcgtgcgg ccgccacgat
     3241 gtcctggtcg gtcgacacgg gcgagacgga gccgacgctc ccggccgtcg tgctcgggat
     3301 ctctcccgtc gcgaggatcg agacgatctg cgcctcgggg agctgcgggt acgacgtgaa
     3361 tcgcgggacg atccgggagg ccgtgccggt gacgccgagc gtgatcgcgt actccttgac
     3421 ctgcgttcgc gcctccagct cgaagtaggg gtcgttcgcc gcggggttcg cgaagaccag
     3481 cttggcgcgc gtgacgtcgt agcgcaggcc gcgaagctcc aggcgccccc cttcggtcgc
     3541 ctcgatcgtc ccgagcagga gcgggtggcc gaacgtgccg cggaccgtca ggtcgccgga
     3601 gccgcgaagt cgggccacgt tgttgcggac ctcgagggcc cccggcggga tcgcgacgcg
     3661 gacctcgagg tcgacgtcgt cgaagcgggt cggctcggga ggagcctccg cggcgccgcg
     3721 ccggccaccg aggagcgact ggagagagag gtcgaggtcc tcggcgtaga ggccccgggt
     3781 catcgtgagc tcgccgcggg ccgagcggag ctcgtcgtcg ccgaggagga cgaggtcgcc
     3841 cgagacggtc gagcgaaggc cggggaacgg ctcgccgcgg agcccgtcga ggtggacgtt
     3901 caggcgcatc ccggtcatgg cgaagccgtc gagggtcgcc gctccggcga ccgcgacgtc
     3961 gccgttccag cgaagggtca ggtcgtctgt cgtgacgcgg cccggcgtca gcaggatcgt
     4021 ccccgagatc gcctccacgg gctcctctcc gggcgacgtg acgagctcga ggtcctccag
     4081 gacgaccctc ccctcgaaga ccggccgcgc cgtcgtcccc gaggccgtgg ccgacaccgc
     4141 catccggcct tcgaggctcg cgacgccgag gaagggcgcg aggaaggccg cctccatcgt
     4201 cccggcggcc cgcacgtcga tcgcgcccgc gccgccggtc tcga
//
LOCUS       DAOONL010000224         4240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25140_length_4240_cov_12.128554, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000224 DAOONL010000000
VERSION     DAOONL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25140_length_4240_cov_12.128554"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLP50_17130"
     CDS             <1..503
                     /locus_tag="PLP50_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPA53318.1"
                     /translation="WTHATQWLERTLDDSANRRLALAESFLAADALLVLQEAVVDGLV
                     VHPEVIRRRLAEELPFLATENVLMAAAKKGGDRQALHERIRVHAQAAGDRLKAAGGEN
                     DLLERISSDPAFGLSREEVAAAADPRLFLGRAPAQVEEFLAAEVDPLLAARAEALAGR
                     AVEVTV"
     gene            539..1198
                     /locus_tag="PLP50_17135"
     CDS             539..1198
                     /locus_tag="PLP50_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPA53319.1"
                     /translation="MALALAAAFLPGCSSRPPARGAPDLAGEGTERGLASWYGAEFAG
                     LPTASGEIFDPTRISAAHRALPLGTIVDVTNEKNGRSVRVRINDRGPFVAGRIVDLSR
                     AAAEEIDAVLDGVVPVTLSVVTLGSGRRTPRGPGGATTTWAVQAGAFADAENAERLKA
                     RLAERYPSPWLEPFQGLTRVKLGPYRSRDEADEACAGLADFGLAGIVVPHDTGTARPG
                     S"
     gene            1208..>1572
                     /locus_tag="PLP50_17140"
     CDS             1208..>1572
                     /locus_tag="PLP50_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPA53320.1"
                     /translation="MIRTCPGCQQKNRVSARHAVAVARCGKCRAELPPPAEPIDADAA
                     LFDEVVREAPVPVLVDFWAAWCAPCRRAAPEVARAAAETAGRALVLKVDTEAHPDLAA
                     RFRVQSIPMFVVLSGGQVVV"
     assembly_gap    1573..1672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1673..2223)
                     /gene="pepE"
                     /locus_tag="PLP50_17145"
     CDS             complement(<1673..2223)
                     /gene="pepE"
                     /locus_tag="PLP50_17145"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPA53321.1"
                     /translation="MTDARNRLFLVSSSTIHGRGYLDHVADALASFLSGVKTVTFVPY
                     ALADRDGYAAKARERFAALGIELLSVHQAPDPRAAVRGADAVFVGGGNTFRLLKWLQD
                     EGLLGPIRDRVAEGMPYVGSSAGTNVACPAIRTTNDMPIVPTANFLALGLVPYQINPH
                     YLDPDPASTHKGETREERLLQYL"
     gene            complement(2256..2456)
                     /locus_tag="PLP50_17150"
     CDS             complement(2256..2456)
                     /locus_tag="PLP50_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53322.1"
                     /translation="MVKLLLWLLLLVVCWPLAIVALVAWPFLWLLSIPFRIVGALLDG
                     LVGLVRALFLLPARLAGGRARA"
     gene            2530..3609
                     /locus_tag="PLP50_17155"
     CDS             2530..3609
                     /locus_tag="PLP50_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HPA53323.1"
                     /translation="MALFEKLTLRSITLPNRIGLAPMCQYSAVDGLANDWHLVHLGAR
                     AAGGAGLVMTEAVAVSPEGRISPGDLGLWADGQVGPLARIASFLAAQGAVPAIQLAHA
                     GRKASTKRPWEGGGRALPREGGWEPICAPSAVPFDEGWQLPEELDTDGMARLAAAFAA
                     AARRAATAGFLVAEIHAAHGYLLHEFLSPIANRRGDPFGGTLENRMRFPLRVVEAVRA
                     AWPSDLPLFVRISATDWVDGGFVPDEAVAFAREAKARGVDLVDCSSGGMHPKAVVPVA
                     PGYQVPFAARIRAEAGISTAAVGLITEPREADEIVAAGRADLVLLGRELLRTPQWPLA
                     AAETLGALVEGPRQYVRAKPYKILK"
     gene            3802..>4240
                     /locus_tag="PLP50_17160"
     CDS             3802..>4240
                     /locus_tag="PLP50_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPA53324.1"
                     /translation="MITRWNDPFRELESFRSQMNRLFGETFPAARTTEEAPGLAAWAP
                     PVDITETPDQLVFQVELPGFSQDDLKLRAENGVLTLEGERRFEKESEKKAYHRVERAY
                     GRFVRAFSLPANVDPEKITAGLVDGILTVELPKREEAKPKSIPI"
BASE COUNT          580 a         1542 c         1463 g          555 t
ORIGIN      
        1 cctggaccca cgccacgcag tggctcgagc ggacgctcga cgactccgcg aaccgccgcc
       61 tcgccctcgc cgagtcgttc ctcgcggccg acgccctcct cgtcctccag gaagcggtcg
      121 tcgacgggct cgtcgtccac cccgaggtga tccgccgccg cctcgccgag gagcttccgt
      181 tcctcgccac cgagaacgtc ctgatggcgg ccgcgaagaa gggaggcgac cgtcaggcgc
      241 tccacgagcg gatccgcgtc cacgcgcagg ccgcgggcga ccgcctgaag gccgcgggag
      301 gcgagaacga cctcctcgag cggatctcct ccgacccggc gttcggcctg tcccgcgagg
      361 aggtcgccgc ggcggccgac ccgcgcctct tcctcggccg cgcacccgcg caggtcgagg
      421 agttcctcgc ggccgaggtc gacccgctcc tcgcggcgcg ggccgaggcg ctggcgggcc
      481 gggcggtcga ggtgacggtc tgaggctcgc ggcgaggacg gcgtcgcgcg cggcgctcgt
      541 ggcgctggcg ctcgcggcgg cgttcctccc cggctgctcg tcccgcccac cggcgcgggg
      601 agcgcccgac ctcgctggcg aggggacgga gcgcgggctc gcctcctggt acggagccga
      661 gttcgccggc ctcccgacgg cgagcggcga gatcttcgac ccgacgcgga tctccgcagc
      721 gcaccgtgcc ctcccgctcg gcacgatcgt cgacgtgacg aacgagaaga acggccggag
      781 cgtccgcgtg aggatcaacg accgcggccc cttcgtcgcc ggccggatcg tcgacctctc
      841 gcgggccgcg gctgaggaga tcgacgccgt cctcgacggc gtcgtccccg tgacgctctc
      901 ggtggtgacg ctcggctccg gacggcggac gccgcgcgga ccgggcggcg ccacgacgac
      961 ctgggccgtc caggccggcg ccttcgcgga tgcggagaac gccgagcggc tgaaggcacg
     1021 gctcgcggag cgctacccgt ccccctggct ggagccgttc caggggctga cccgcgtcaa
     1081 gctggggccg tatcgctcgc gcgacgaggc ggacgaggcc tgcgccggcc tggccgactt
     1141 cggcctcgcc gggatcgtcg tcccgcacga caccgggacg gcgcggccgg gctcctagaa
     1201 tcggcgggtg atccgcacct gcccgggctg ccagcagaag aaccgcgtct ccgcgcgcca
     1261 cgccgtcgcg gtggcccgtt gcgggaagtg ccgcgccgag ctgccgccgc ccgccgagcc
     1321 gatcgacgcc gacgccgcgc tcttcgacga ggtcgtccgg gaggcgccgg tgccggtcct
     1381 cgtcgacttc tgggccgcct ggtgcgcccc gtgccggcga gccgcgccgg aggtggcgcg
     1441 cgccgccgcc gagaccgccg ggcgcgccct cgtcctgaag gtcgacaccg aggcgcatcc
     1501 cgacctcgcg gcccgcttcc gcgtccagtc gatcccgatg ttcgtcgtcc tctccggcgg
     1561 gcaggtcgtc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgaggta
     1681 ctggaggagc cgctcctcgc gcgtctcccc cttgtgcgtg gaggccgggt cggggtcgag
     1741 gtagtgcggg ttgatctggt agggcacgag accgagcgcg aggaagttcg ccgtcgggac
     1801 gatcggcatg tcgttcgtcg tccggatcgc cgggcacgcg acgttcgttc ccgcgctcga
     1861 gccgacgtac ggcatcccct cggcgacgcg gtcccggatc gggccgagga gcccttcgtc
     1921 ctgcagccac ttcaggagcc ggaacgtgtt gccgccgccg acgaagaccg cgtcggcgcc
     1981 gcggacggcg gcccgcgggt cgggagcctg gtggacgctc aggagctcaa tcccgagggc
     2041 tgcgaagcgc tcgcgcgcct tcgcggcgta gccgtcacgg tcggcgaggg cgtacgggac
     2101 gaacgtcacc gtcttcacgc ccgagaggaa cgaggcgagg gcgtcggcga cgtggtcgag
     2161 gtagccgcgg ccgtggatgg tggagctcga gacgaggaag agccggtttc gcgcgtcggt
     2221 catgccggga ggttagcgtg ccgggaggct ccccgtcagg cccgggcccg cccgccggcg
     2281 aggcgcgccg ggaggaggaa gagcgcccgg acgagcccga cgagaccgtc caggagcgcc
     2341 ccgacgatcc ggaacgggat cgagaggagc cagaggaacg gccacgccac gagcgcgacg
     2401 atcgcgagcg gccagcagac gacgagcagg aggagccaca ggaggagctt gaccatctcc
     2461 cttcctgata cggcggccgg accgcgaggt tcccgctgcg ccgcctcgcc ctgccgtacg
     2521 ctcctccata tggcgctctt cgagaagctc accctccgtt cgatcaccct gccgaaccgg
     2581 atcggcctcg cccccatgtg ccagtactcc gccgtcgacg gcctcgcgaa cgactggcac
     2641 ctcgtccacc tcggcgcgcg cgccgccggc ggcgccggcc tcgtcatgac cgaggcggtc
     2701 gccgtttcgc ccgaaggacg gatcagcccc ggcgatctcg gcctctgggc cgacgggcag
     2761 gtcgggccgc tcgcgcgcat cgcatccttc ctcgcggcgc agggagccgt accggctatc
     2821 cagctcgcgc acgccggacg caaggcctcc acgaagcgcc cctgggaggg tggcggtcgc
     2881 gcgctcccgc gggaaggggg ttgggagccg atctgcgcgc cgagcgccgt cccgttcgac
     2941 gaggggtggc agcttccgga ggagctcgac accgacggga tggctcgcct cgccgcggct
     3001 ttcgccgccg ccgcgcgccg agccgccacc gccgggttcc tcgtcgccga gatccacgcc
     3061 gcccacggat acctcctcca cgagttcctc tccccgatcg ccaatcggcg gggcgacccc
     3121 ttcggcggca ccctcgagaa ccggatgcgc ttcccgctcc gcgtcgtcga ggccgtgcgc
     3181 gccgcctggc cctccgacct gcccctcttc gtccggatct ccgcgaccga ctgggtcgac
     3241 ggcggcttcg tcccggacga ggcggtcgcc tttgcccgcg aggcgaaggc ccgcggcgtc
     3301 gacctcgtcg actgctcctc gggcgggatg cacccgaagg ccgtcgtccc cgtcgcgccc
     3361 ggctaccagg tgccgttcgc cgcccgaatc cgcgcggagg ctggaatctc caccgccgcg
     3421 gtcggcctca tcaccgaacc tcgcgaggcg gacgagatcg tcgccgcggg ccgagcggac
     3481 ctcgttctgc tgggccggga gctcctccgg accccgcagt ggcccctcgc cgccgcggag
     3541 acgctcgggg ccctcgtcga gggccctcgg cagtacgtcc gggcgaagcc atacaagata
     3601 ctcaaataaa ataagttaca gtacagaccc cgaagagctc ggagtgaagc accctcggag
     3661 agggacctct ctcgttcatt ttgtccttac gtacttgaca aactcacaat cagatcctaa
     3721 atttgccgcg gacctgaggc ctccaatgaa gaaatggagc ccacggacca gaattcggat
     3781 cttcagaggg aggtaagcac catgatcacc cgttggaacg accccttccg cgagctcgag
     3841 tccttccgca gccagatgaa ccgcctcttc ggcgagacgt tcccggccgc ccggacgacg
     3901 gaagaggcgc cggggctcgc cgcctgggcc ccgccggtcg acatcaccga gacgccggac
     3961 cagctcgtct tccaggtcga gctcccgggt ttctcgcagg acgacctcaa gctccgcgcc
     4021 gagaacgggg tcctgacgct cgagggcgag cgcaggttcg agaaggagag cgagaagaag
     4081 gcgtaccacc gcgtcgagcg ggcctacggc cgcttcgtcc gggccttctc cctgcccgcg
     4141 aacgtcgacc cggagaagat caccgcggga ctcgtcgacg ggatcctgac cgtcgagctc
     4201 ccgaagcggg aagaggcgaa gccgaagtcg atcccgatcg
//
LOCUS       DAOONL010000225         4217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25332_length_4217_cov_15.443056, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000225 DAOONL010000000
VERSION     DAOONL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25332_length_4217_cov_15.443056"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1117)
                     /locus_tag="PLP50_17165"
     CDS             complement(230..1117)
                     /locus_tag="PLP50_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA53325.1"
                     /translation="MTFRPARLLASALAALLLAGAVAETAEGATKRKRRTRRKPAPIP
                     AVPRPHDPFEVTIPTADGVRLYATWKPAPTGPAAPAVLLVHAFSRERRDLAELADELV
                     ARGFSTLAVDLRGHGESVWKGGARIGLSPSLQTSPSAFPRDVEAACAWLKGRASRVGV
                     VGLSLSGNLAALATAAGWAEAGVAVSAYADRLPALAGSRPTTPRGLLVLASEADPGRV
                     ESARTLDAAGQEPKAVAVFPGAAHALDLLTLEPDAKKAAIEWLEARLSPIPPLPVATA
                     APEAPEAAPAEPPSAEGVE"
     gene            1247..2242
                     /gene="nadA"
                     /locus_tag="PLP50_17170"
     CDS             1247..2242
                     /gene="nadA"
                     /locus_tag="PLP50_17170"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006511638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPA53326.1"
                     /translation="MASPPNPLPTLEIPLPAELPDAILRLKEERDALILAHYYQEPEI
                     QDLADFVGDSLELARKGKASDRPVIAFCGVHFMAETSKILTPEKTVVVPDLEAGCSLA
                     DGCPAPLFAEWLRKYPGHTVASYINCSAAVKALSDVIVTSSSAEAILGQIPDPVVFAP
                     DKNLAAWLEKKLGRKFVVWPGTCVVHETFSEKRILDLIVRNPNARLVAHPECDERILA
                     HAHFVGSTSALLKHVTESEHRSFIVATEEGILHQMRRAAPGKELIAAPPDESCSCNTC
                     PHMKRNTLEKLWLALRDLSPAIEVPEETRVAAKRSLDRMLELTAGKTFVPALGRS"
     gene            2256..>4217
                     /locus_tag="PLP50_17175"
     CDS             2256..>4217
                     /locus_tag="PLP50_17175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPA53327.1"
                     /translation="MTEDAPPRPRPRVPLGPLLGGLVVLVAGAVAAHRVLPEWQPLGV
                     RKADAIRTATEEMTAAGATLLAPTATVQNLLASNELERAFRRLGREAPSWLARHGALA
                     TWTVRGQLRVPGVGTGPAEIAIARDGTFRRLHFTSGSLFRIGDPQTQMQETRERFATA
                     VLARLARGQELGASSDFESGNTVVRVWPLQGRGGGPAEVVTKTSPSAAMLMLARELGD
                     PDIGARYEKNLFRYLAIAIPLFGVFGIGVLVLFGVLLFRRRLGFRIGTALAVLALAAM
                     LLSGAFFDVGGQQGFVAGVLVAGRLIGVVVLVGYWAIAEALLRDSKTGFRTSLDAFAS
                     GRLTPRLGRAVLGGLGLGAAVAGLRLLFAAGVAAAGVPGVVPQSPSYSLPLFGGLANA
                     FLEGPYAAASFVLAVALLRNLLRPERADALGAAAFALLSSLSFSYEPWGALLVLTLAE
                     SAVLLLAFRSHGLTGLLVAATAPGLFRDLLAALRFPGELVLPLLLAAAALAALAWIGV
                     GATRRPEREDEAKTDAPEVVKRLEAERRVKYEMDLLSRMQLALLPDAPPDVPGLQIAA
                     RTIVATEAGGDLYEFLVDEEGALWIAAGDVAGHGYSCGIQGAMVKACLLSLVKAGRRP
                     GEVLAEIDRVLRAGRAARLFTTLVLVRIEP"
BASE COUNT          509 a         1634 c         1482 g          592 t
ORIGIN      
        1 acgcctcgcc gtcctcggcc ccccgcgtga gccactcgcg cagcatcggg tcgagatcga
       61 gccagggcag cccgacggtc tcgatgtcat acacgagagt cttcacagcg gacctccaat
      121 cggagggcgg ggggtccggg gggccgcgcg gagctcaggc cccccggtgg ggacgagagt
      181 cttcacagcg gtcctccaat cggagggcgg gagggccggg gggccgcgct cattccacgc
      241 cttcggcgct cgggggctcc gccggggcgg cctccggtgc ctcgggcgcc gccgtcgcga
      301 cggggagcgg cgggatcggc gagaggcgcg cctcgagcca ctcgatcgcg gccttcttcg
      361 cgtccggctc gagcgtgagg aggtcgagag cgtgagcggc ccccggaaag acggcgacgg
      421 ccttcggctc ctgtccggcg gcgtcgaggg tccgcgccga ctcgacgcgc cccggatcgg
      481 cctcggacgc gaggacgagg aggccgcgcg gcgtcgtcgg ccgcgagccc gcgagcgccg
      541 ggagccggtc ggcgtacgcc gagacggcga ctcccgcctc ggcccagccc gccgccgtcg
      601 cgagggcggc caggttcccc gagagtgaga ggccgacgac gccgacacgc gaggcacggc
      661 ccttcagcca ggcgcaggcc gcctcgacgt cgcgcgggaa ggcggacgga gacgtctgga
      721 gagacgggga gaggccgatc cgcgcgcccc ccttccacac cgactcgccg tggccgcgca
      781 ggtcgacggc gagcgtcgag aagccgcggg ccacaagctc gtcggcgagc tcggccaggt
      841 cccgccgctc gcgggagaag gcgtggacga ggaggacggc cggcgccgcg gggccggtcg
      901 gcgccggttt ccaggtcgcg tagagacgga cgccgtccgc cgtcgggatc gtcacctcga
      961 acgggtcgtg cggacggggg acggccggga tcggcgccgg cttgcggcgc gtccgccgct
     1021 tccgcttcgt cgccccctcg gccgtctcgg cgaccgcgcc cgcgaggagg agcgccgcga
     1081 gggcggaggc gaggagacgg gccgggcgga acgtcatccc tgcgcgacct ccgtcccgcc
     1141 tctcgtgtcg atggtcgtcg tctcgacaga gctcatggtc gcacccccgg aagctccgga
     1201 tggtagcaga gggctcgcgg ccctttcgga gccgtagaat gcccgcgtgg cgagccctcc
     1261 gaaccctctc ccgacgctcg agatccccct ccccgccgag ctgccggacg cgatcctccg
     1321 cctcaaggag gagcgcgacg ccctgatcct ggcgcactac taccaggagc cggagatcca
     1381 ggacctcgcg gacttcgtcg gcgactcgct cgagctggcc cggaagggga aggcctccga
     1441 caggccggtc atcgcgttct gcggtgtcca tttcatggcc gagacgtcga agatcctcac
     1501 ccccgagaaa accgtcgtcg tccccgacct cgaggcgggc tgctcgctcg ccgacggctg
     1561 cccggcgccg ctcttcgccg agtggctccg gaagtacccc ggccacaccg tcgcctcgta
     1621 catcaactgc tcggcggcgg tgaaggcgct ctccgacgtc atcgtcacct cctcctcggc
     1681 cgaggcgatc ctgggccaga tccccgaccc ggtcgtcttc gcccccgaca agaacctcgc
     1741 cgcctggctc gagaagaagc tcggccggaa gttcgtcgtc tggccgggga cgtgcgtcgt
     1801 ccacgagacc ttctccgaga agcggatcct cgacctgatt gtgaggaacc cgaacgcccg
     1861 cctcgtcgcc caccccgagt gcgacgagcg gatcctcgcg cacgcccatt tcgtcggctc
     1921 cacctccgcg ctgctgaagc acgtcaccga gtccgagcac cgctcgttca tcgtggcgac
     1981 cgaggagggg atcctccacc agatgcgccg ggccgcgccg gggaaggagc tgatcgcggc
     2041 gcctcccgac gagagctgct cctgcaacac ctgcccgcac atgaagcgga acaccctgga
     2101 gaagctctgg ctcgcgctcc gcgacctctc gcccgcgatc gaggtccccg aggagacgcg
     2161 ggtcgcggcg aagcgctcgc tcgacaggat gctcgagctg acggcgggga agacgttcgt
     2221 ccccgcgctc ggccgctcct gagccgcccg acgtcgtgac ggaagacgcc cctccgcgtc
     2281 cgaggccccg cgtcccgctg gggccgcttc tcggcgggct cgtcgtcctc gtcgcgggcg
     2341 ccgtcgccgc gcatcgcgtc cttcccgagt ggcagccgct cggcgtgagg aaggccgacg
     2401 cgatccggac ggcgaccgag gagatgacgg ccgccggagc cacgctcctc gcgccgacgg
     2461 cgacggtcca gaacctcctc gcctcgaacg agctcgagcg ggccttccgg cggctcggcc
     2521 gggaggcgcc ctcctggctc gcccgccacg gcgccctcgc gacctggacc gtgcggggcc
     2581 agctccgcgt ccccggcgtc gggacgggtc cggccgagat cgccatcgcg cgcgacggca
     2641 cgttccgacg gctccatttc acgagcggca gcctcttccg gatcggcgac ccccagacgc
     2701 agatgcagga gacgcgggag cgcttcgcca cggccgtcct cgcccgtctc gcccgcggcc
     2761 aggagctcgg cgcctcctcc gacttcgaga gcgggaacac cgtcgtccgc gtctggcccc
     2821 tccagggccg tggcggcggg ccggcggaag tcgtcacgaa aacctcgccg agcgcggcga
     2881 tgctgatgct cgcccgcgag ctgggcgacc ccgacatcgg ggcgcggtac gagaagaacc
     2941 tcttccgcta tctcgcgatc gcgatccccc tcttcggcgt cttcggcatc ggcgtcctcg
     3001 tcctcttcgg cgtcctcctc ttccggaggc gtctcggatt ccggatcggc accgccctcg
     3061 ccgtcctcgc cctcgccgcg atgctcctgt ccggcgcctt cttcgacgtc ggcggccagc
     3121 agggcttcgt ggcaggcgtc ctcgtcgccg ggcggctcat cggcgtcgtc gtcctcgtgg
     3181 gctactgggc catcgcggag gcgctcctcc gggactcgaa gaccggcttc cggacgagcc
     3241 tcgacgcctt cgcctccggg cggctcacgc cgcgcctcgg tcgcgcggtc ctgggtgggc
     3301 tcgggctcgg cgccgccgtc gccggactgc gcctcctctt cgccgccggc gtcgccgccg
     3361 ccggggtccc cggcgtcgtc ccgcagtccc cttcctactc cctcccgctc ttcggcggcc
     3421 tcgcgaacgc cttcctcgag gggccgtacg ccgcggcgtc cttcgtcctc gccgtggcgc
     3481 tcctccggaa cctcctgcgc cccgagaggg ccgacgccct cggcgccgcc gcgttcgcgc
     3541 tcctctcttc cctctcgttc tcctacgagc cgtggggcgc gctcctcgtc ctgacgctcg
     3601 cggagtcggc ggtcctcctg ctcgcgttcc gctctcacgg cctgaccggc ctcctcgtcg
     3661 ccgccacggc gcccggcctc ttccgcgacc tcctcgccgc cctccgcttc ccgggggagc
     3721 tcgtccttcc gctcctcctc gctgcggcgg cgctcgcggc gctcgcctgg atcggcgtcg
     3781 gggcgacccg ccgcccggag cgcgaggacg aggcgaagac ggacgcgccg gaggtcgtga
     3841 agcggctcga ggcggagcgg cgcgtgaagt acgagatgga cctcctctcg cggatgcagc
     3901 tcgcgctcct ccccgacgcg ccgcccgacg tgcccgggct ccagatcgcg gcccggacga
     3961 tcgtggccac cgaggcgggg ggcgacctct acgagttcct cgtcgacgag gagggggcgc
     4021 tctggatcgc ggcgggcgac gtcgccggac acggctattc ctgcggcatc cagggggcga
     4081 tggtgaaggc gtgcctcctc tcgctcgtca aggcggggcg ccgcccgggc gaggtcctcg
     4141 ccgagatcga ccgcgtcctc cgcgcggggc gcgccgcgcg cctcttcacg acgctcgtcc
     4201 tcgtgaggat cgagccg
//
LOCUS       DAOONL010000226         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25630_length_4177_cov_17.070354, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000226 DAOONL010000000
VERSION     DAOONL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25630_length_4177_cov_17.070354"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLP50_17180"
     CDS             complement(<1..263)
                     /locus_tag="PLP50_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPA53328.1"
                     /translation="MTEAVEERRLFADFLRRRGLKTTRERTALFEEIFATHHHFDADD
                     LVVRLRGKGKKVSRATVYRTLDLLYDCGLVSRVRLNDEKYRYER"
     gene            377..796
                     /locus_tag="PLP50_17185"
     CDS             377..796
                     /locus_tag="PLP50_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPA53329.1"
                     /translation="MKSYRKELTFHLPKRIGFVNVTPEVEAAVRESGISEGLCLVNPM
                     HITASVFVNDDERGLLADFERFFEALVPHEPVRSWRHNDTGEENADAHVKRQLMGREA
                     VLAVTGGRLDLGPWEQVFYGEWDGRRAKRVLIKIIGE"
     gene            complement(803..2689)
                     /gene="aroF"
                     /locus_tag="PLP50_17190"
     CDS             complement(803..2689)
                     /gene="aroF"
                     /locus_tag="PLP50_17190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPA53330.1"
                     /translation="MTERNQPDGRDGACERRIVRAGYQGREGSFGARAAARCGRPVAL
                     PTFEALLDALAEGTVDEALLPAVDRVIGPVVEALGPLGAAIDGGARLAVLGEIEVPMR
                     LVLAAPRGVALEEIEELHSQLPVLRQCEGLLARLGARAVPAEDTAQAAATVARAGGRR
                     GAVCSEEAALEAGLVPLLEDVSDVRPNGTRFWRLARAGGEGSDQPLRVLRVPTERLAA
                     HLKQSGIEVFSSGPGAGFALVGDALVATAAAAVREAKAQGATWLGTPPDLARRPRVEA
                     LPRATEVRPATVVTVGGFRVGGGARAVIAGPCTVESEEQIRRLARAVARAGATALRGG
                     VFKPRTSPYSFQGHGLHALRWLKEAGERVGLPVVTEVMAPAQVGPVAEVADVLQIGAR
                     NCQNYDLLKEAGAAGRPILLKRGFGTTVDEWLASAEYLLDAGCEQVMLCERGIRTFEQ
                     STRGTLDLGGLLAARQLTHLPLLADPSHAAGRKELVPGLARAAWGAGVDGLIVEVHDA
                     PESAWCDGPQALLPEDLERLCDEFGLLPGATPSVERVRTAIDAVDREIGRLVARRLEL
                     CRRVADAKRAAGVPLRDAAREETVMARYLETPGIDEGSARRLAESLIAMGLTVEGWSA
                     EGAA"
     gene            complement(2899..3261)
                     /locus_tag="PLP50_17195"
     CDS             complement(2899..3261)
                     /locus_tag="PLP50_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPA53331.1"
                     /translation="MNLILRVLVNALALGAAAYLVPGIRAGGVESVLLIALVFGVLNA
                     LVRPFLKLLSCPLLVLTLGLFTLVLNAVMLMLTAWLGQKLGIDFRVDGFWPAFLGALV
                     VSIVSVVLSWVLIDEKKG"
     gene            3353..3784
                     /locus_tag="PLP50_17200"
     CDS             3353..3784
                     /locus_tag="PLP50_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53332.1"
                     /translation="MDRKYRQRGYQDNAPSTPRSGGPFDDRPARLEGAPRGRSAGRPS
                     EEVFRCRTCHEKNDPEVALAALCVKCRAPLHACAQCRHFDGGAPFQCRQPIPAPISAK
                     SRANECTFYEPAVTLDLTGRKATDTPDQARSAFDALFGKKK"
     gene            3863..>4177
                     /locus_tag="PLP50_17205"
     CDS             3863..>4177
                     /locus_tag="PLP50_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53333.1"
                     /translation="MRGHTRIGLLAAGFSLLLGGCVSTTWHPAEDLAPGVQASVSPTE
                     VAVLRSLPTAPHQSLGTIEVWLSGRHSTAAILGKVREKAAAVGADAVVLMPGTTLFSE
                     PMS"
BASE COUNT          597 a         1583 c         1475 g          522 t
ORIGIN      
        1 cgctcgtagc ggtacttctc gtcgttcagc cgcacgcggg agacgaggcc gcagtcgtag
       61 aggaggtcga gcgtccggta gaccgtcgcc cggctcacct tcttcccctt gccccgcagc
      121 ctcacgacga ggtcgtcggc gtcgaagtgg tggtgcgtcg cgaagatctc ttcgaacagc
      181 gcggtccgct cgcgggtcgt cttcagcccg cgccgccgca gaaaatcggc gaaaagtcgg
      241 cgttcctcga cggcttccgt catggcggag acgatatcgc aaccggcgcc cgcgagggag
      301 gggtgatctc ggatcggcgc cccggctcgc gccagcccgc gagggaggct agcctcggcg
      361 ccgcacggag gcgcgcatga agtcgtaccg caaagagctg acgttccacc tcccgaagcg
      421 gatcggcttc gtgaacgtga cgcccgaggt cgaggccgcg gtccgcgaga gcggtatctc
      481 cgagggactc tgcctcgtca acccgatgca catcacggcc tcggtcttcg tgaacgacga
      541 cgagcgcggc ctcctcgcgg acttcgagcg cttcttcgag gcgctcgtcc cgcacgagcc
      601 ggtccgctcc tggcggcaca acgacaccgg agaggagaac gccgacgccc acgtgaagcg
      661 ccagctcatg gggcgcgagg ccgtcctcgc cgtcaccggc gggaggctcg acctcggccc
      721 ctgggagcag gtcttctacg gcgagtggga cgggcggcgc gcgaagcggg tcctgatcaa
      781 gatcatcggg gagtgaccct cctcaggccg ccccctcggc ggaccagcct tcgacggtca
      841 accccatcgc gatgagcgac tcggcgaggc ggcgcgccga gccctcgtcg atcccgggcg
      901 tctcgaggta ccgggccatc acggtctcct cacgggccgc atcgcggagc gggacgcccg
      961 cggcccgctt ggcgtcggcg acgcgccggc agagctcgag gcggcgggcg acgaggcgtc
     1021 cgatctcgcg gtcgacggcg tcgatggcgg tccgcacgcg ctccaccgag ggggtcgcgc
     1081 ccgggaggag gccgaactcg tcgcagagcc gctcgaggtc ctccggcagg agcgcctggg
     1141 ggccgtcgca ccaggccgac tccggggcgt cgtggacctc gacgatgagg ccgtcgacgc
     1201 cggcgcccca cgcggcccgc gcgagcccgg gcacgagctc cttccgcccc gccgcgtgag
     1261 aggggtccgc gaggagcggg aggtgcgtca gctgccgtgc cgcgaggagc cccccgaggt
     1321 cgagggtccc gcgggtggac tgctcgaacg tccggatgcc gcgctcgcag agcatcacct
     1381 gctcgcaccc cgcgtcgagg aggtactcgg ccgacgcgag ccactcgtcg accgtggtgc
     1441 cgaagccgcg cttgaggagg atcggccgtc ccgcggctcc ggcttccttg aggaggtcgt
     1501 agttctggca gttccgcgcg ccgatctgga ggacgtcggc gacctctgcg accggcccga
     1561 cctgcgccgg cgccatcacc tccgtgacga ccggcaggcc gacgcgctcg cccgcctcct
     1621 tcagccagcg gagcgcgtgg agcccgtgcc cctggaagga gtagggcgag gtgcgcggct
     1681 tgaagacgcc gccccggagc gcggtggcgc cggcccgcgc caccgcccgc gcgaggcgcc
     1741 tgatctgctc ctcggactcg acggtgcagg gccccgcgat gacggcccgc gccccgccgc
     1801 cgacccggaa gccgccgacc gtcacgaccg tcgcggggcg cacctcggtg gcgcgcggga
     1861 gcgcctcgac ccggggccgc cgtgcgaggt cgggcggcgt cccgagccac gtggctccct
     1921 gcgccttcgc ctcgcgcacc gccgccgcgg ccgtcgccac cagcgcgtcg ccgacgagag
     1981 cgaagcccgc ccccggcccc gaggagaaga cctcgatccc ggactgcttc aggtgcgccg
     2041 ccagacgctc cgtcgggacg cgcaggacgc ggagcggctg atcgctcccc tccccgcccg
     2101 cccgggcgag cctccagaag cgcgtcccgt tcgggcggac gtccgagacg tcctccagga
     2161 gcggcacgag gcccgcctcg agggccgcct cctcggagca gaccgcgccg cgcctcccgc
     2221 ccgcgcgcgc gacggtcgcc gcggcctgcg ccgtgtcttc cgccggcacg gcgcgcgccc
     2281 cgaggcgggc gaggagcccc tcgcactgcc ggaggacggg gagctgggag tggagctcct
     2341 cgatctcctc gagcgcgacg ccgcgcggcg ccgcgaggac gaggcgcatc ggcacctcga
     2401 tctcgccgag gaccgcgagg cgtgcgcctc cgtcgatggc cgcgccgagg ggcccgagcg
     2461 cctcgacgac cggcccgatg acccgatcga ccgccggcag gagcgcctcg tcgacggtcc
     2521 cctcggcgag ggcgtcgagg agcgcctcga aggtcgggag cgccaccggc cgcccgcagc
     2581 gtgccgccgc gcgggcgccg aaggagccct cgcgcccctg gtagccggcg cgcacgatgc
     2641 gccgctcgca cgccccgtcg cgcccgtccg gctggttccg ctccgtcatc gtcgcctcct
     2701 tctcgccttc gccggaaacg aagaagcccc cctccaccga tctggcggag ggaggcttcg
     2761 ggatgccctt cgcggtggcc gtgcctacac gcccacccct ccgcggaacg gcgaatagac
     2821 gccgccggtc gccgtaaacg ggagggaaag gccgttcttc atcacgtcgc cgggaacgat
     2881 aacccggagg tcaagccctc accccttctt ctcgtcgatc aggacccagg agaggacgac
     2941 ggagacgatc gacacgacga gggccccgag gaaggcgggc cagaagccgt cgacgcggaa
     3001 gtcgatgccg agcttctggc cgagccacgc cgtgagcatg agcatcaccg cgttcaggac
     3061 gagagtgaag agaccgagcg tcaggacgag gagcgggcag gagaggagct tgaggaacgg
     3121 gcggacgagt gcgttcagga cgccgaagac gagcgcgatc aggaggaccg actcgacgcc
     3181 tccggcgcgg attccgggca cgaggtacgc cgccgccccg agcgcgagcg cgttcacgag
     3241 cactctgagg atcaggttca tcggctctct ctccttccag cacgggaacg atacctcgcg
     3301 gcgttgacgc gcgcccttcc gctgcggcac gctcggggcc gaggtgacct ccatggaccg
     3361 gaagtaccgc cagcgcggct accaggacaa cgccccgtcg acgccccgga gcggcggccc
     3421 attcgacgac cgccccgccc ggctcgaggg ggcgccgcga ggacggagcg ccggccggcc
     3481 cagcgaagag gtcttccgct gccgcacgtg ccacgagaag aacgaccccg aggtcgctct
     3541 cgccgcgctc tgcgtcaagt gccgcgcgcc cctccacgcc tgcgcgcagt gccgccactt
     3601 cgacggagga gccccgttcc agtgccggca gccgatcccg gcgccgatct ccgcgaagtc
     3661 ccgcgcgaac gagtgcacgt tctacgagcc cgccgtcacg ctcgacctga ccgggcgcaa
     3721 ggcgaccgac acccccgacc aggcccgctc cgccttcgac gccctcttcg gcaagaagaa
     3781 gtagggcggc gcggcggctg tcctccggac gaggcggtcc caccggcatg tcctttgctc
     3841 gcacgcctct ggaggtaccc tcatgagagg acacacgcgg atcgggctcc tcgccgcagg
     3901 cttctctctc ctgctcggcg gctgcgtttc gacgacctgg cacccggccg aggacctcgc
     3961 ccccggggtt caggcctcgg tctccccgac cgaggtcgcc gtccttcgtt cccttcccac
     4021 ggctccgcac cagtccctcg gcacgatcga agtctggctc tcgggccgtc acagcaccgc
     4081 cgcgattctc ggcaaggtcc gggagaaggc ggcggccgtc ggcgccgacg cggtcgtgct
     4141 gatgccgggg acgaccctct tctccgagcc catgagc
//
LOCUS       DAOONL010000227         4165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25731_length_4165_cov_11.707786, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000227 DAOONL010000000
VERSION     DAOONL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25731_length_4165_cov_11.707786"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLP50_17210"
     CDS             <1..962
                     /locus_tag="PLP50_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPA53334.1"
                     /translation="HALGAAAVVNATGAWADRLRAEVGGRPRIRPLRGSHLLFPQWRF
                     PTAQAVTFRHPVDARPLFAYPWEGLTLLGTTDLDHPGSLDDEPSISVPEVDYLLEGAR
                     SAFPSLALSVRDAVSSFAGVRPVVGTGKTRPSEESREHVLWDEEGLVTVTGGKLTTFR
                     LIAVETLRLLRGRIPALARVRADAGVFRPCDAPLPEAAGLDAATARRLAGRHGGAAAE
                     VVAGARPGELEPVAGTPWLWAEIRHAARSERVVRLDDLLLRRVRVGLLLPDGGASLLP
                     RVAAICREELAWDDERWRMEEQDYLARWRASHSAPAASAALCA"
     gene            959..2515
                     /locus_tag="PLP50_17215"
     CDS             959..2515
                     /locus_tag="PLP50_17215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPA53335.1"
                     /translation="MSGERLLAIDHGTQSVRALLFDLSGNLLARSRVPIEPYVPGPPG
                     FAEQDPELYWRSVCAATRGLLAQPDVSAGSIAGLAVTTQRATMVATDAQGRPLRPAIV
                     WLDQRRTDGVPPVRGLWGAAFRLSGMSGTVAYFQAEAEAHWLARHEPETWRKTERYLF
                     LSGFLAHRLTGRFVDSTGGQVGYVPFDYRRLRWASRLDWKWEALPVRREQLPELVPPG
                     GRLGELSAAAAEATGLPKGLPVIAAAADKACEVLGAGCLEPHVACLSYGTTATINTTH
                     RRYVEPLPLLPPYPSAVPGAYSLEIQIYRGFWMVTWFKEQFGQLEEAEAREKGLPAEA
                     LFDELVSKVPPGSMGLVLQPYWSPGVRRPGPEAKGAVIGFGDVHTRAHLYRAILEGLA
                     YALREGKERSERRSGVPITELRVAGGGAQSDAAMQITADVFGLPAARPHVTEASGLGA
                     AIDAAVGLGLHPTFETAVASMTRVGRVFVPEPSTSGLYDRLYRRVYRRMYERLRPLYA
                     EIREITGYPP"
     regulatory      2579..2647
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            2660..2998
                     /locus_tag="PLP50_17220"
     CDS             2660..2998
                     /locus_tag="PLP50_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HPA53336.1"
                     /translation="MPWLFLVLAGLLEVGWAVGLKFTDGFRRPLPSLLVAASMAGSVV
                     LLAWAVRSIPIGTAYAVWVGIGALGAVVFGVVLFDEPLPPLRALFVALLVVAIAGLKW
                     TSGPAGDAGS"
     gene            3024..>4165
                     /locus_tag="PLP50_17225"
     CDS             3024..>4165
                     /locus_tag="PLP50_17225"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HPA53337.1"
                     /translation="MTAADVLLPRKGAILVKWGFEKYRSGFRRITRRSARHFEAADWH
                     GVQQDMVERLALYTSVVRRVVASLEEVFGEERRSPELWSAMKREYSWLMGGHGDLELA
                     ETFFNSVTRKVFATVGVDPKREFVHLGSDTGLPPPGAVPVRTYPGALSTGTVVGEVVD
                     DYSFAAPWEDPARDARRVAAEVDRAVAGAWGEPGFDFVELLEPVFYRGKGAYVVGRVV
                     KGRRSLPLVLALANPGGRVAVDAVLLDEDEVSIVFSFTRSYFHVDVEAPAGVVRFLKS
                     ILPRKPLSELYVSLGYNKHGKTELYRDLLRHLERSGDRFERARGERGMVMAVFTMPGY
                     DVVFKVIRDRFGPAKSVTREEVRERYQFVFEHDRAGRLVDVQEYELL"
BASE COUNT          497 a         1513 c         1529 g          626 t
ORIGIN      
        1 agcacgcgct cggcgccgcg gccgtcgtga acgccaccgg cgcgtgggcc gaccgcctcc
       61 gcgccgaggt gggcggccgc ccgcgaatcc gtcccctgcg ggggagccat ctcctctttc
      121 cgcagtggcg attcccgacg gcgcaggccg tgacgttccg ccacccggtc gacgcgcgtc
      181 ccctcttcgc ctatccgtgg gagggcctca cgctcctcgg gacgaccgac ctcgaccacc
      241 ccgggtcgct cgacgacgag ccgtccattt ccgtgcccga ggtcgactac ctcctcgagg
      301 gagcgcggag cgcgtttccc tctctcgcgc tctccgtccg ggacgcggtc tcgagcttcg
      361 cgggcgtgcg gccggtcgtc gggacgggga agacgcgtcc ctccgaggag agccgcgagc
      421 acgtcctctg ggacgaggaa ggcctcgtca cggtgaccgg cgggaagctg acgacgttcc
      481 gcctcatcgc cgtcgagacg ctccgcctgc tgcgcgggag gattccggct ctcgcccgcg
      541 tccgcgccga cgcgggcgtc tttcgcccgt gcgacgcgcc tctgccggag gccgcgggcc
      601 tcgacgccgc gacggcgcgc cgcctcgcgg gccgtcacgg cggcgcggcg gcggaggtcg
      661 tcgccggggc gcggcccggc gagctcgagc cggtcgcggg gaccccctgg ctctgggcgg
      721 agatccggca cgccgcgcgc tccgagcggg tcgtccggct cgacgacctc ctcctgcggc
      781 gcgtgcgggt cgggctcctc ctgccggacg gcggggcgtc gctcctcccg cgcgtcgcgg
      841 cgatctgccg ggaggagctc gcgtgggatg atgagcgctg gcggatggaa gagcaggact
      901 acctcgcgag gtggcgcgcg agccactcgg cgcccgcggc gtcggccgct ctctgcgcgt
      961 gagcggcgag cggctcctcg cgatcgacca cgggacgcag agcgtccggg ccctcctctt
     1021 cgacctgtcg gggaacctcc tcgcgcggag ccgcgtcccg atcgagccgt acgtcccggg
     1081 tcctcccggc ttcgccgagc aggacccgga gctctactgg cggtcggtct gcgccgcgac
     1141 gcgggggctc ctcgcgcagc cggacgtctc tgccggctcg atcgccggcc tcgccgtgac
     1201 gacgcagcgg gcgacgatgg tggcgacgga cgcgcagggg aggccgctcc ggccggcgat
     1261 cgtctggctc gaccagcgga gaacggacgg cgtccctccg gtccggggtc tctggggggc
     1321 ggccttccgc ctctccggga tgtccgggac ggtggcctac ttccaggcgg aggccgaggc
     1381 gcactggctc gctcggcacg agccggagac gtggcggaag acggagcggt acctcttcct
     1441 ctccggcttc ctcgcgcacc ggctgacggg tcgcttcgtc gactccacgg gcgggcaggt
     1501 cggctacgtc ccgttcgact accggaggct ccgctgggcg tcgcgcctcg actggaagtg
     1561 ggaagctctg ccggtccgcc gggagcagct cccggagctc gtcccgccgg gaggccggct
     1621 cggcgagctg tccgcggccg ccgcggaggc gacaggcctg ccgaagggtc tcccggtgat
     1681 tgcggccgcc gccgacaagg cgtgcgaggt cctcggggcc ggatgcctcg agccgcacgt
     1741 cgcctgcctg agctacggga cgacggcgac gatcaacacg acgcaccgcc gctacgtcga
     1801 gccgctcccg ctcctgccgc cgtacccgtc ggccgtcccc ggcgcctaca gcctcgagat
     1861 ccagatctac cgcggcttct ggatggtgac ctggttcaag gagcagttcg gccagctcga
     1921 ggaggcggag gcgcgcgaga aggggctccc cgcggaggcg ctcttcgacg agctcgtgtc
     1981 gaaggtcccc cccgggtcga tgggcctcgt cctccaaccc tactggtctc cgggcgtgag
     2041 gcgccccggg ccggaggcaa aaggggccgt catcggtttc ggcgacgtcc acacccgcgc
     2101 gcacctctac cgggcgatcc tggaagggct cgcctatgcc cttcgcgagg ggaaggaacg
     2161 ctcggagagg cggagcggcg tcccgatcac ggagctgcgc gtggcgggcg ggggagcgca
     2221 gagcgacgcg gcgatgcaga tcaccgcgga cgtcttcggc cttcccgcgg cgcgaccgca
     2281 cgtgaccgag gcgtccggcc tcggcgccgc gatcgacgca gcagtcggac tcgggctcca
     2341 cccgactttc gagaccgcgg tcgcctcgat gacacgggtc ggtcgcgtct tcgttcccga
     2401 gccttcgacc tcgggcctct acgaccgcct ctaccgccgc gtctaccgcc ggatgtacga
     2461 gcggctccgg ccgctctacg ccgagatccg cgagatcacc gggtacccgc cgtgattgcg
     2521 gcccgaggtg gcccggggcg gagttgaacg acccgggccg cgatgctacg gtccgcgccg
     2581 cgcgcggccc cgggccgtgt cgcccggacg agacgcgccg tacccggtca ggacaagacg
     2641 acgcggaaag gagcctccca tgccctggct cttcctcgtc ctggccggcc tcctcgaggt
     2701 ggggtgggcc gtcggcctga agttcaccga cggcttccgc cgccccctcc cgtcgctcct
     2761 cgtcgccgcc tcgatggccg ggagcgtcgt cctcctcgcg tgggcggtcc gctcgattcc
     2821 gatcgggacg gcttacgcgg tctgggtcgg gatcggcgcc ctcggagccg tcgtattcgg
     2881 ggtcgttctc ttcgacgagc cgctcccgcc cctccgggcg ctcttcgtgg ctctcctcgt
     2941 cgtcgcgatc gcggggttga agtggacctc ggggccggcg ggagacgccg ggagctgaac
     3001 gcctgccgta tcctcgcggg acgatgaccg ccgcggacgt cctcctcccg cgcaaggggg
     3061 cgatcctcgt caagtggggc ttcgagaagt atcgctccgg cttccggagg atcacccggc
     3121 gctcggcgcg ccacttcgag gcggccgact ggcacggcgt ccagcaggac atggtcgagc
     3181 gcctcgccct ctacacgagc gtcgtccggc gtgtcgtggc ctcgctcgag gaggtcttcg
     3241 gagaggagcg ccgctcgccc gagctctggt cggcgatgaa gcgcgagtac tcctggctga
     3301 tgggcgggca cggcgacctc gagctcgcgg agacgttctt caactcggtc acgcggaagg
     3361 tcttcgcgac cgtcggcgtc gatccgaagc gtgagttcgt ccacctcggg agcgacacgg
     3421 gccttccccc gccgggcgcc gtgccggtga ggacctaccc cggtgcgctc tcgaccggaa
     3481 cggtcgtcgg ggaagtggtc gacgactact ccttcgcggc cccgtgggag gacccggcgc
     3541 gcgatgcgcg gcgggtggcg gcggaggtcg accgggcggt cgcgggggcg tggggagagc
     3601 ccggcttcga cttcgtggag ctgctggagc cggtcttcta ccgcggcaag ggagcgtacg
     3661 tcgtcggccg cgtcgtgaag ggccggcgtt cgcttcctct ggtcctggcc ctcgcgaatc
     3721 cgggagggcg ggtggccgtc gacgccgtcc tcctcgacga ggacgaggtc tcgatcgtct
     3781 tcagcttcac ccggtcctac ttccacgtcg acgtcgaggc gccggcgggc gtcgtccggt
     3841 tcctcaagtc gatcctcccg cgcaagccgc tgtccgagct ctacgtctcg ctcgggtaca
     3901 acaagcacgg gaagacggag ctctaccgcg acctcctccg gcacctcgag cggtcgggcg
     3961 accggttcga gcgggcgcgc ggcgagcgcg ggatggtgat ggccgtcttc acgatgccgg
     4021 gctacgacgt cgtcttcaag gtgatccgcg accgcttcgg cccggccaag agcgtgacgc
     4081 gcgaggaggt acgggagcgc taccagttcg tcttcgagca cgaccgggcc gggcggctcg
     4141 tcgacgtcca ggagtacgag ctcct
//
LOCUS       DAOONL010000228         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_25762_length_4161_cov_19.996834, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000228 DAOONL010000000
VERSION     DAOONL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25762_length_4161_cov_19.996834"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLP50_17230"
     CDS             complement(<1..1437)
                     /locus_tag="PLP50_17230"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPA53338.1"
                     /translation="MSTATETLAGKPLSSNPHLLAWVEECARLCKPDKVVWCDGSEEE
                     KERLTALAVSTNVLIPLNQEKLPGCYLHRSDPADVARVEHLTFICTKTKEEAGPNNNW
                     MAPDEAYRKLGTLYDGSMKGRTMYVVPYVMGPIGSPQAKVGVELTDSVYVVLNMRIMT
                     RMGTRALRMLGDSADFNRGLHSMLDLNPERRFICHFPEDNTIWSIGSGYGGNVLLGKK
                     CLALRIGSYLGRQEGWMAEHMLIMGVEEPSGETTYVAAAFPSACGKTNFSMLIPPSSF
                     NGWKVWTVGDDICWMRPGPDGRLWAINPEAGYFGVAPGTNYATNPNAMESIKRDTLYT
                     NVAMTPDGDVWWEGKDSTPPEELTDWRGRPWQRGSSEKAAHPNSRFTAPAANNPALSP
                     RVNDRNGVPISAIIFGGRRSTTVPLVLQSFNWTHGVYLGATMGSETTAAAVGKEGVVR
                     RDPMAMLPFCGYDMGRYFEHWLNMEGKVA"
     gene            1499..2368
                     /gene="asd"
                     /locus_tag="PLP50_17235"
     CDS             1499..2368
                     /gene="asd"
                     /locus_tag="PLP50_17235"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPA53339.1"
                     /translation="MSDAAPPLSLRLLGLYPKKAGSAAVGLLARTRLPRALRAPLLGR
                     FAASYRVDLGEAERPLPEYASFLDFFTRRLKAGLRLQEPAVPGGVNSPVDGALIASGR
                     VEQGTLIQAKGLPYRLDELLASDPLAARFEGGAYATLYLSPRDYHRIHVPCPGRVLAV
                     GRVEGELWPVNEASTAFTPGLYVRNRRAYWIAEGTGEDEGLDVAAVMVAATHVGGVVV
                     DARWLGGRTLGRRDRLAVDGLPCASGDDLGTFELGSTVVLLVGGARASAFDWSRPHGP
                     VRVGERLGAFAGR"
     regulatory      2429..2642
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2744..>4161
                     /locus_tag="PLP50_17240"
     CDS             2744..>4161
                     /locus_tag="PLP50_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA53340.1"
                     /translation="MNRIPLVLLLPLALSTSALGQAPRSGPAPVSTEVVVTAEATPAD
                     ASSLGVATTVLDRTDIERSRATTVADLLRSVPGVDVAQSGGPGGVTSLFLRGANSNSA
                     LVLVDGVKLNSPYFGGVDLSSLGTANLERVEVVRGPFSALYGSEAIGGVIQLFTRKAD
                     AEGLSGRAHFGLGNASTREGGVNAAWRRGTIGVTAGYRRTTAGGDLPNEFFAGTDLSA
                     ALDLRLSEGVRTGVTVRRETSRTGIPFEGATTTPRRATTADTTLISVPLSLALGARTT
                     LEAAGTFATDEPTFSDPDEPWGFTFSETRARRTGGRLVLSHERSANRISIGADLERTK
                     VDNEDVYGVQLDGLTTRTWSVFAEDRLSLARDRFVVTAGVRRDEHDAFGASTNPRVAL
                     SWKASSAFRLRAAAGSAFRAPATGELYYPFSGNPALEPERSVSYEAGAEWSLGGGASL
                     EATVFRSDVKDLIRYDFAFQKNV"
BASE COUNT          528 a         1531 c         1457 g          645 t
ORIGIN      
        1 ggcgaccttc ccctccatgt tcagccagtg ctcgaagtag cgccccatgt cgtagccgca
       61 gaaggggagc atcgccatcg ggtcgcggcg gacgacccct tccttcccga cggcggcggc
      121 cgtcgtctcc gagcccatcg tcgcgccgag gtagacgccg tgcgtccagt tgaacgactg
      181 gaggacgagc ggcaccgtcg tcgagcggcg gccgccgaag atgatcgcgg agatcggcac
      241 gccgttccgg tcgttcacgc gcggtgagag cgccgggttg ttcgcggccg gggcggtgaa
      301 gcggctgttc gggtgggctg ccttctccga cgagccgcgc tgccagggcc ggccgcgcca
      361 gtccgtcagc tcctccggcg gggtgctgtc cttcccctcc caccagacgt ctccgtccgg
      421 cgtcatcgcg acgttcgtgt agagcgtgtc gcgcttgatc gactccatcg cgttcgggtt
      481 cgtcgcgtag ttcgtgcccg gcgcgacgcc gaagtagccc gcctcggggt tgatcgccca
      541 gagccggccg tccggcccgg ggcgcatcca gcagatgtcg tcgccgacgg tccagacctt
      601 ccagccgttg aagctcgacg gcgggatgag catcgagaag ttcgtcttgc cgcaggcgga
      661 ggggaaggcc gcggcgacgt acgtcgtctc gccgctcggc tcctcgaccc ccatgatgag
      721 catgtgctcg gccatccagc cctcctggcg gccgaggtag gagccgatcc ggagcgcgag
      781 gcacttcttg ccgaggagga cgttcccgcc gtagcccgag ccgatcgacc agatcgtgtt
      841 gtcctcgggg aagtggcaga tgaaccgccg ctcggggttc aggtcgagca tcgagtggag
      901 gccgcggttg aagtcggccg agtcgccgag catccggagg gcgcgcgtcc ccatgcgcgt
      961 catgatccgc atgttcagga cgacgtagac gctgtcggtc agctcgacgc ccaccttggc
     1021 ctgcggcgag ccgatcggcc ccatgacgta cgggacgacg tacatcgttc gccccttcat
     1081 ggagccgtcg tagagcgtgc cgagcttccg gtaggcctcg tccggcgcca tccagttgtt
     1141 gttcggcccc gcctcctcct tcgtcttcgt gcagatgaag gtgaggtgct cgacgcgcgc
     1201 gacgtcggcc gggtcggagc ggtggaggta gcagccgggg agcttctcct ggttgagcgg
     1261 gatcaggacg ttcgtcgaga cggcgagggc cgtcagacgc tccttctcct cctcgctgcc
     1321 gtcgcaccag acgaccttat cgggcttgca gagccttgcg cactcctcga cccaggcgag
     1381 gaggtggggg ttggaggaca ggggcttgcc ggccagggtc tcggttgcag tgctcatcgc
     1441 gctctcctcc tcggggcagt caatccggac gcggaaagac ccctatcata atcggctcat
     1501 gtccgatgcg gctccgcccc tctccctgcg gctcctcggc ctctatccga agaaggcggg
     1561 ctcggccgcg gtcgggctgc tggcgaggac gcgactgccg cgtgcgctgc gcgcgccgct
     1621 ccttgggcgc ttcgccgcct cctaccgcgt cgacctcggc gaggcagaga gaccgctacc
     1681 cgagtacgcc tcgttcctcg atttcttcac gcgcaggctg aaggcgggcc tccgcctcca
     1741 ggagccggcg gtcccgggcg gcgtcaactc gccggtcgac ggcgccctca tcgcctccgg
     1801 gcgggtcgag cagggcacgc tcatccaggc gaaggggctt ccgtaccgcc tcgacgagct
     1861 cctcgcctcc gatccgctcg cggcgcgctt cgagggcggg gcgtacgcga cgctctatct
     1921 ctcgccgcgc gactaccacc ggatccacgt tccgtgcccg gggcgcgtcc tcgccgtcgg
     1981 gcgtgtcgag ggggagctct ggccggtgaa cgaagcttcg accgcgttca ctccgggcct
     2041 ctacgtgagg aaccggcgcg cgtactggat cgccgaaggg acgggcgagg acgaaggcct
     2101 cgacgtcgcg gccgtgatgg tcgccgcgac ccacgtcggg ggcgtcgtcg tcgacgcgcg
     2161 ctggctcggg ggccggacgc tcggacgccg cgaccgcctt gccgtcgatg ggcttccgtg
     2221 cgcctcggga gacgatctcg ggacgttcga gctcggctcg acggtcgtcc tcctcgtcgg
     2281 gggtgcgcgg gcctctgctt tcgactggag ccgcccgcac ggcccggtcc gggtcgggga
     2341 gcggctcggg gcgttcgccg gacgctgacg ccgggcggtc ccgcgggccg gcgcgcgtcg
     2401 cgccccgggt gctatcctcc gcgaccggtt ctttcaacgg atctcgggtc ccccgtcctc
     2461 cggggggcga gagagggaag cgggtgagat tcccgcgcgg cccccgccac tgtgaccggg
     2521 gacgcgaagg acgaaatccc actgggggcg agcgatccgc gacgcgggtt tcgcctccgg
     2581 gaaggggtcc ttccgcggac gatccgggag ccaggagacc tgcccgaggc ccatcgcaac
     2641 ccgccgcgcc gtctccgggg ctcgagaggt cgcgggagac gcctccgcgc gcgcctcccg
     2701 ccccttccga tgggacggca ggccgcagcc gaacggaggc ctcttgaacc gcatccctct
     2761 cgtcctcctc ctccctctcg ccctgtctac gtccgccctc ggccaggcgc cccggtcggg
     2821 cccggctccc gtctcgaccg aggtcgtcgt caccgccgag gcgacacccg ccgacgcctc
     2881 ctcgctcggc gtcgcgacga cggtcctcga ccggaccgac atcgagcgct cccgcgcgac
     2941 gacggtcgcc gacctcctgc ggagcgtccc cggcgtcgac gtcgcgcaga gcggagggcc
     3001 gggcggcgtc acgtcgctct tcctgcgcgg ggcgaactcc aactcggcgc tcgtcctcgt
     3061 cgacggcgtc aagctgaaca gcccctactt cggaggcgtc gacctctcct cgctcggcac
     3121 cgcgaacctg gagcgcgtcg aggtcgtccg cggccccttc tcggcgctct acggctcgga
     3181 ggcgatcggg ggggtcatcc agctcttcac ccggaaggcc gacgcggagg gcctctccgg
     3241 ccgggcgcac ttcggcctcg ggaacgcctc gacacgcgag gggggcgtca acgccgcctg
     3301 gcggcgcggc acgatcggcg tgaccgccgg ctaccggcgg accaccgccg gcggcgacct
     3361 tccgaacgag ttcttcgccg ggaccgacct ttccgccgcg ctcgacctgc ggctctcgga
     3421 gggcgtccgg accggcgtca ccgtccggcg cgagacctcg cgaacgggca tccccttcga
     3481 gggggcgacg acgacgccgc ggcgcgccac gacggccgac acgacgctca tctccgttcc
     3541 gctctcgctc gctctcggcg cgcggacgac gctcgaggcg gccggcacct tcgcgaccga
     3601 cgagccgacg ttctccgacc cggacgagcc ctggggcttc acgttctccg agacgcgcgc
     3661 ccggcgcacc gggggacgac tcgtcctctc ccacgagcgc tccgcgaacc ggatctccat
     3721 cggcgccgac ctcgaacgga cgaaggtgga caacgaggac gtgtacgggg tccagctcga
     3781 cggcctgacg acgcgcacct ggtcggtctt cgccgaggac cgcctctccc tcgcccggga
     3841 ccgcttcgtc gtcacggccg gcgtccgcag ggacgagcac gacgccttcg gcgcgtcgac
     3901 gaacccgcgc gtcgccctct cctggaaggc ctcctccgcg ttccggctcc gcgccgcggc
     3961 gggctcggcg ttccgagccc ccgcgacggg cgagctctac tacccgttct ccgggaaccc
     4021 ggcgctcgag ccggagcgct ccgtctcgta cgaggcgggg gcggagtgga gcctcggcgg
     4081 tggcgcctcc ctcgaggcga ccgtcttccg gagcgacgtg aaggacctga tccggtacga
     4141 cttcgcgttc cagaagaacg t
//
LOCUS       DAOONL010000229         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_26033_length_4128_cov_18.109747, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000229 DAOONL010000000
VERSION     DAOONL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26033_length_4128_cov_18.109747"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..247)
                     /locus_tag="PLP50_17245"
     CDS             complement(8..247)
                     /locus_tag="PLP50_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPA53341.1"
                     /translation="MPTVTVSSKGQIVIPLDLRRELGLREGERLEVTAKADGLVLTRV
                     RVRAGRPGWRSWGGSLAGSGALEEHRKEHAREAKR"
     gene            complement(480..1211)
                     /locus_tag="PLP50_17250"
     CDS             complement(480..1211)
                     /locus_tag="PLP50_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA53342.1"
                     /translation="MSSRPEKSPLPNGRAIIEMDGIRKVYDTGKVKVEALKGVDLRIE
                     KGEFVAIVGPSGSGKSTLMNLLGCLDTPSGGDYFIGGEKVAGLTRDALAEVRNRRVGF
                     VFQAFNLLPQISAFENVEMPLVFGGVAPKVRRAKVAELLGKVGLGDRMDHKPTELSGG
                     QMQRVAIARALAMDPDIVLADEPTGNLDTTSGTDIMGVFLDLWRSGSTLVVITHDMAL
                     ARRASRIVEIRDGRIVSDRAAEEAA"
     gene            complement(1221..2465)
                     /locus_tag="PLP50_17255"
     CDS             complement(1221..2465)
                     /locus_tag="PLP50_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA53343.1"
                     /translation="MKVIFHLFLQSATLGRKRAFLTIAAIAWGTVAILLLLAFGEGLK
                     RQLGSNARAMGENIGVMWMSETTKAWKGMNPGRPIRVRIDDLPVLKSRLPELEDLIGE
                     AIFWRTNLTSGRKTVAGRVKGVSVAYGEARKQYPVEGGRFLSPLDEAERRRVIFLGNE
                     LAEDLFGKEDPVGKTMLVAGAPFTVVGVMQKKTQMGAYAGMDKDHSIVPISTWKALFG
                     RPTVNNLVLRVSDPAKMKDAIARTREVIAAKYGFDPSDERALPTWDTVESQKTTRNMT
                     VGIQIFLGIIGALTLLIGGVGVANIMYAVVKERTREIGVKMALGAKAGWITGPFVLEG
                     LSYTLVGGAAGMLIATLMVLAMSFIPTDGNQVMEFLGTPKLSPAIGAATASILGVIGL
                     LAGWFPARRAAAVDPAATLRYE"
     gene            complement(2477..3703)
                     /locus_tag="PLP50_17260"
     CDS             complement(2477..3703)
                     /locus_tag="PLP50_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA53344.1"
                     /translation="MLRDALLQLHRDLKSQKLRTLLTVFGLVWGTVAVTLLLAFGAGL
                     KKQLIKSTAGLGDRICISWPGLTSIPYQGLGKGRRIRITDEDLQAVRPRVDGLKRMSS
                     EYSDGWKMVYNLRTFPVDVSGVSPEFGEMRNLIPVPGGRFLNDLDVRDQRRVVFLGNK
                     VAEDVFGKGTDPVGKTVLFNGSPFLVIGVLTAKQQDSSYNSRDSDLSWIPGTTFRALT
                     GRKYLSNFVYQPASALESKAVTESLRSALSRRLKFDPEDKEAFSVWDVTEQFQFFEVF
                     MLAFSAFLGIVGSLTLVVGGIGVSNIMNVVVDERTKEIGIKMALGAKSGAILRQFLVE
                     TLIVTAIGGLAGFAISLGICAVFPASLEEYVGRPEVSPVVAGITVGILGVIGLLAGWF
                     PARSAARLDPVVAMKM"
     gene            complement(3803..>4128)
                     /locus_tag="PLP50_17265"
     CDS             complement(3803..>4128)
                     /locus_tag="PLP50_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HPA53345.1"
                     /translation="QQKDGATLFDVEIELDPGQKIVLRAGYSANADLIIREKKDVVTI
                     PERLVTFEDGGKKAFVELPGKDPKAEPKKVEIQTGISDGLNIEVTSGLKKGDQVVQRP
                     PKQIS"
BASE COUNT          603 a         1446 c         1409 g          670 t
ORIGIN      
        1 cagacgctca ccgcttcgcc tctctcgcgt gctccttccg atgctcctcg agcgcacccg
       61 agccggcgag ggagcccccc caggagcgcc agccgggccg gcccgcccgg acccggaccc
      121 gagtgaggac gagcccgtcg gccttcgccg tcacctccag ccgctcgcct tcgcggagcc
      181 cgagctccct ccgaaggtcg agcgggatga cgatctggcc cttcgacgat acggtcacgg
      241 tcggcatggc gggtcctccg cgctcatctt actccaaggg taagacgctc cgacgcgggg
      301 gccttcactc gtcgtcccgg tgacggtcgc cgagacaccg cctacgatga ttcacgctcg
      361 agcatgcggg agaagtgcgc cctccccctc cccgaccccg cggacgtcct cgcggcgtag
      421 ttccacgtcc gcggctctct cgcagctcgc gtggaggcag ccgagctgca gcgccccccc
      481 tacgccgctt cttccgccgc ccggtcgctg acgatccgtc cgtcgcggat ctcgacgatc
      541 cggctcgcgc ggcgggcgag cgccatgtcg tgcgtgatca cgacgagcgt cgagccgctc
      601 ctccagaggt cgaggaagac tcccatgatg tcggtccccg aggtggtgtc gaggttgccg
      661 gtcggctcgt cggcgaggac gatgtcgggg tccatcgcga gggcgcgggc gatggcgacg
      721 cgctgcatct ggccgcccga gagctccgtc ggcttgtggt ccatccggtc gccgaggccg
      781 accttcccga ggagctcggc gaccttcgcg cggcggacct tcggggccac gcctccgaag
      841 acgagcggca tctcgacgtt ctcgaaggcc gagatctgcg gcagcaggtt gaacgcctgg
      901 aagacgaagc cgacgcggcg gttccgcacc tcggcgaggg cgtcgcgcgt cagcccggcc
      961 accttctcgc cgccgatgaa gtagtcgccc ccgctcggcg tgtcgaggca gccgaggagg
     1021 ttcatgaggg tcgatttgcc ggagccggac gggccgacga tcgcgacgaa ctcccccttc
     1081 tcgatcctca ggtcgacgcc cttcagcgcc tcgaccttca ccttgccggt gtcgtagacc
     1141 ttccggatcc cgtccatctc gatgatggcg cgtccgttcg ggagcgggct cttctcgggt
     1201 cggctcgaca tggcaggctc ctactcgtag cgaagcgtgg cggcggggtc gacggcggcg
     1261 gcgcggcgcg ccgggaacca gccggcgagg aggccgatga cgccgaggat cgaggcggtc
     1321 gcggcgccga tcgcgggaga gagcttcggc gttccgagga actccatcac ctggttcccg
     1381 tcggtcggga tgaagctcat ggcgaggacc atcagggtcg cgatgagcat ccccgcggcg
     1441 cccccgacga gggtgtacga gagcccctcc aggacgaacg ggccggtgat ccagcccgcc
     1501 ttcgcgccga gggccatctt cacgccgatc tcgcgcgtcc gctccttgac gaccgcgtac
     1561 atgatgttcg cgacgccgac gccgccgatc aggagcgtga gggcgccgat gatcccgagg
     1621 aagatctgga tcccgacggt catgttccgc gtcgtcttct gcgactcgac ggtgtcccag
     1681 gtcgggagcg cccgctcgtc cgacgggtcg aagccgtact tggccgcgat gacctcgcga
     1741 gtccgcgcga tcgcgtcctt catcttcgcc gggtcgctca cccgaagcac gaggttgttc
     1801 accgtcggcc ggccgaagag ggccttccag gtcgagatcg ggacgatgga gtggtccttg
     1861 tccatcccgg cgtaggcgcc catctgcgtc ttcttctgca tcacgccgac gaccgtgaag
     1921 ggcgcgccgg ccacgagcat cgtcttcccg accgggtctt ccttgccgaa gaggtcctcg
     1981 gccagctcgt tgccgaggaa gatcacccgg cgccgctcgg cctcgtcgag cggcgagagg
     2041 aaccgtcccc cctcgacggg gtactgcttg cgggcctcgc cgtacgcgac gctgacgccc
     2101 ttcacgcgcc cggcgaccgt cttccggccg gaggtcaggt tcgtccgcca gaagatcgcc
     2161 tcgccgatga ggtcctcgag ctcggggagc cgcgacttca ggaccggaag gtcgtcgatg
     2221 cggacccgga tcggccggcc cgggttcatc cccttccagg ccttcgtcgt ctcgctcatc
     2281 cacatcacgc cgatgttctc gcccatcgcg cgcgcgttcg acccgagctg ccgcttcagg
     2341 ccctcgccga acgcgaggag gaggaggatc gcgacggtcc cccaggcgat cgcggcgatc
     2401 gtcaggaacg cgcgcttgcg cccgagcgtg gcggactgga ggaagaggtg gaagatgact
     2461 ttcacggccc gcctccctac atcttcatgg cgacgaccgg gtcgaggcgc gcggccgagc
     2521 gcgccgggaa ccagccggcg aggaggccga tgacgccgag gatgccgacc gtgatccccg
     2581 cgacgacggg agagacctcc ggccggccga cgtactcctc gaggctcgcg gggaagacgg
     2641 cgcagatgcc gagcgagatc gcgaaaccgg cgaggccgcc gatggcggtg acgatgagcg
     2701 tctcgacgag gaactgccgg aggatcgcgc ccgacttggc gccgagcgcc atcttgatgc
     2761 cgatctcctt cgtcctctcg tcgacgacga cgttcatgat gttcgagacg ccgatcccgc
     2821 cgaccacgag cgtcagcgac ccgacgatcc cgaggaaggc cgagaacgcg agcatgaaga
     2881 cctcgaagaa ctggaactgc tcggtgacgt cccagaccga gaaggcctcc ttgtcctcgg
     2941 ggtcgaactt cagccgccgc gacagcgccg accggaggct ctccgtgacc gccttcgact
     3001 cgagggccga ggcgggctgg tagacgaagt tcgagaggta cttccgcccc gtcagcgcgc
     3061 ggaacgtcgt ccccgggatc caggagaggt cgctgtcgcg gctgttgtag ctcgagtcct
     3121 gctgcttggc cgtcaggacg ccgatgacga ggaacggcga gccgttgaag aggacggtct
     3181 tgccgaccgg gtccgtcccc ttgccgaaga cgtcctcggc gaccttgttg ccgaggaaga
     3241 cgacgcggcg ctggtcccgc acgtcgaggt cgttcaggaa ccgtccgccc ggcaccggga
     3301 tcaggttccg catctcgccg aactcgggcg agacgccgga gacgtcgacg gggaaggtcc
     3361 ggaggttgta gaccatcttc cagccgtcgc tgtactcgct cgacatccgc ttcaggccgt
     3421 cgacgcgcgg ccgcacggcc tggaggtcct cgtcggtgat ccggatccgg cgccccttgc
     3481 cgagcccctg gtacgggatc gacgtgaggc cgggccagga gatgcagatc cggtcgccga
     3541 ggccggccgt cgacttgatc agctgcttct tcagcccggc gccgaaggcg aggaggagcg
     3601 tgaccgcgac cgtcccccag acgagcccga agaccgtcag gagcgtccgg agcttctggc
     3661 tcttcaggtc gcggtggagc tgcagcagcg cgtcgcggag catcggcctt ccttcctcgt
     3721 ctctttcctc gggcgtgggg ccccgctctc ggggcgcgga gggggcgcgg ggggacccgg
     3781 cttcgggttc ccccggaact cgtcagctga tctgcttcgg cggccgctgg acgacctggt
     3841 cgcccttctt caggcccgag gtcacctcga tgttgaggcc gtcggagatg ccggtctgga
     3901 tctcgacctt cttcggctcg gccttcgggt ccttgccggg gagctcgacg aacgccttct
     3961 tgccgccgtc ctcgaacgtc acgaggcgct cggggatcgt cacgacgtcc ttcttctcgc
     4021 gaatgatcag gtcggcattc gccgagtaac cggcgcgcag gacgatcttc tgccccgggt
     4081 cgagctcgat ctcgacgtcg aagagcgttg cgccgtcctt ctgctgcg
//
LOCUS       DAOONL010000230         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_26552_length_4069_cov_18.340309, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000230 DAOONL010000000
VERSION     DAOONL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26552_length_4069_cov_18.340309"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLP50_17270"
     CDS             complement(<1..965)
                     /locus_tag="PLP50_17270"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPA53346.1"
                     /translation="MAKIRPFPASRPVPSRAREVASVPYDVIDTKEARALAEGNPASF
                     LHVCRPEIDLPEGTSLYAEEVYAKGRENLDRFESDGTLVPDPTPRLLVYRQTWRGQKQ
                     DGLVAACSVEDYDADVIKKHEKTRKDKEDDRTKHLVVQAAHAEPVFLTYRASDVVDDR
                     VAKVTSAPPEIDFEAVDGVRHSLWLVPEEEAAFFVDAFAAIPVLYVADGHHRSKSASR
                     ACAALVEKGPVPADHPVRWFPAAIFPDRELRILPYNRLVKDLLGRTPEGFLAEVKKSF
                     HVSVATAAEPERKGEVRMYLDGRWYTLTPKEPVPADDPIRSLDVS"
     gene            1042..1284
                     /locus_tag="PLP50_17275"
     CDS             1042..1284
                     /locus_tag="PLP50_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPA53347.1"
                     /translation="MKIYVGNLPYEVDAPRLGEIFGAFGETASAAVVLDRTTGRSKGY
                     GFVEMADDDARKAILALNRTQLDGRTIWVNEARAKG"
     gene            complement(1298..3187)
                     /locus_tag="PLP50_17280"
     CDS             complement(1298..3187)
                     /locus_tag="PLP50_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA53348.1"
                     /translation="MTAPDGNAEGREKPPKVRRLKALVPDLLELARPRRKLLALGFVL
                     MAAGRAAGLVLPASTKVLIDEVVAKSRTDLLAPLLLAIGAATLVQAATSFALAQLLSK
                     GAQRLIAELRTRVQSHVTRLPVAYFDGQKTGNLVSRVMNDVEGIRNLVGTGVVELAGG
                     LLTSLLALGLMVTLSPVLTGLTLLFLGVFVAGLFAALGALRPIFRERSKIHGEVSGRL
                     TETFAGIRVVKGYHGEVREDAVFGDGVTRLLSNVLRTITGTSALSAGATVLLGGVGAV
                     VMWVGTRQILSGALTVGGLFTYTILLGFLVAPVFQIVGIGSQLTEALAGLERTKEVLA
                     ESREDADPRRAVDVPPLRGEVVFEEVRFAYSEGKEVLHGVSFRAEPGSVTALVGPSGS
                     GKSTIIGLVAAFASPTAGRVLVDGVDLSTVRLSSYRSQLGVVLQESFLFDGTIRENVA
                     FSRPSATEGEVVAACRVARVDEFALGFEKGYETVVGERGVKLSGGQRQRVSIARALLA
                     DPRILILDEATSSLDTESEAFIQQGLAELLRGRTTFVIAHRLSTIRRADQILVVDGGA
                     IVERGRHDALMAAGGRYAEMVTRQAGIGVELFGGPAEAPRDEEDGSSRPSPAGPFSIL
                     GPGRE"
     gene            complement(3184..>4069)
                     /locus_tag="PLP50_17285"
     CDS             complement(3184..>4069)
                     /locus_tag="PLP50_17285"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPA53349.1"
                     /translation="TGRPVADISFSPEDPFDLAPLAKAKGIPAIVDCGVSPGLSGLAV
                     GRAAARLDNLDDVRIFVGGLPAARHWPFEYRSVFSPTDVVEEYTRPSRCVEGGEVVIR
                     PALSGVEPLDVPGVGTLEAFYTDGLRTLLSTVKARNLSEKTLRYPGHADRMRMLRETG
                     FFSGEPVAVNGVPVVPRRLTEALLFHAWRRPEDEAELTFLRVVCEGRHGGRRVRRTFE
                     LLDRTDPKTGDTSMARTTGFPCATGARLLLEGTFTTPGVFPPELLAGDDALYGRFLAD
                     LAARGVAVEETEEELVGG"
BASE COUNT          617 a         1422 c         1485 g          545 t
ORIGIN      
        1 atcgagacgt cgagcgagcg gatcgggtcg tcggccggga ccggctcctt cggcgtcagg
       61 gtgtaccagc gcccgtcgag gtacatccgg acctcgccct tccgctccgg ctcggccgcc
      121 gtggcgaccg agacgtggaa cgacttcttc acctcggcga ggaagccctc cggggtccgg
      181 ccgaggaggt ccttcacgag ccggttgtag ggaaggatcc ggagctcgcg gtccgggaag
      241 atcgccgcgg ggaaccagcg gaccgggtgg tcggccggga ccggcccctt ctccacgagc
      301 gccgcgcagg cgcgagaggc ggacttggag cggtggtgcc cgtcggcgac gtagaggacc
      361 gggatcgcgg cgaaggcgtc gacgaagaag gcggcctcct cctccgggac gagccagagc
      421 gagtggcgca cgccgtcgac cgcctcgaag tcgatctccg ggggcgccga ggtcaccttc
      481 gcgacgcggt cgtcgacgac gtccgaggcc cggtaggtca ggaagacggg ctcggcgtgg
      541 gcggcctgga cgacgaggtg cttcgtccgg tcgtcttcct tgtccttgcg ggtcttctcg
      601 tgcttcttga tgacgtcggc gtcgtagtcc tcgaccgagc aggcggcgac gaggccgtcc
      661 tgcttctgtc cccgccaggt ctggcggtaa acgaggaggc ggggcgtcgg gtcggggacg
      721 agcgtgccgt ccgactcgaa gcggtcgagg ttctcgcggc ccttcgcgta gacctcctcg
      781 gcgtagaggg aggtcccttc cgggaggtcg atctcgggcc ggcagacgtg aaggaaagag
      841 gcggggttcc cctcggcgag ggcccgggcc tccttcgtgt cgatgacgtc gtagggaacg
      901 gacgcgacct ctcgggcgcg ggagggaacg gggcgggagg cggggaaggg gcggatcttc
      961 gccatgtcgc gccgagctta gcaggagctt ccgcgtgctt ccctccccgc tccgcgggac
     1021 ggtgtaggag aatcggggcg aatgaagatc tacgtcggga acctccccta cgaggtcgac
     1081 gcgcctcgcc tgggggagat cttcggggcc ttcggcgaga cggcctcggc cgccgtcgtc
     1141 ctcgaccgga cgacgggccg ctccaaggga tacggtttcg tcgagatggc cgacgacgac
     1201 gcccggaagg cgatcctcgc cctcaaccgg acccagctcg acggccggac gatctgggtg
     1261 aacgaggccc gggcgaaggg ctgatcggcc tcgcctctca ctcccgaccg gggcccagga
     1321 tcgagaacgg gccggccggc gacggtctcg acgagccgtc ctcctcgtcc ctcggcgcct
     1381 ccgccggacc gccgaagagc tccaccccga tcccggcctg ccgcgtgacc atctccgcgt
     1441 agcggcctcc ggcggccatc agcgcgtcgt gacgccctcg ctccacgatc gctcccccgt
     1501 cgacgacgag gatctggtcg gccctccgga tcgtcgagag gcgatgggcg atgacgaacg
     1561 tggtccgccc gcgcagcagc tccgcgagcc cctgctggat gaacgcttcc gactccgtgt
     1621 cgaggctcga ggtcgcctcg tcgaggatca ggatccgcgg gtcggcgagg agggcacgcg
     1681 cgatcgagac ccgctggcgc tggcctcccg agagcttcac gccccgctcg ccgacgaccg
     1741 tctcgtagcc cttctcgaag ccgagggcga actcgtccac tctcgcgacg cggcacgcgg
     1801 cgacgacctc cccctcggtg gcggaaggcc tcgagaacgc gacgttctcc cggatggtgc
     1861 cgtcgaagag gaacgactcc tgaaggacga ctccgagctg cgagcggtag ctcgagagcc
     1921 gcacggtcga gaggtcgacg ccgtcgacga ggacgcgccc ggccgtcggc gaggcgaacg
     1981 ccgcgacgag gccgatgatc gtcgacttcc ccgatccgga gggaccgacg agcgcggtca
     2041 cgctccctgg ctccgcccgg aacgagacgc cgtggaggac ctccttcccc tcggaatacg
     2101 cgaagcggac ctcctcgaag acgacctcgc cgcggagagg agggacgtcg acagcgcgcc
     2161 gcgggtcggc gtcctcccgg gactcggcga ggacctcctt cgtccgctcg aggcccgcga
     2221 gagcctcggt cagctggctc ccgatcccga cgatctggaa gaccggggcc acgaggaacc
     2281 cgaggaggat cgtgtaggtg aagagaccgc cgacggtcag ggcgcccgac aggatctgcc
     2341 gcgtcccgac ccacatcacg acggccccga cgccgccgag gaggacggtc gccccggccg
     2401 agagcgccga cgtcccggtg atcgtccgga ggacgttcga gaggaggcgc gtcacgccgt
     2461 ccccgaagac ggcgtcctct cggacctcgc cgtggtaccc cttcacgacg cggatgccgg
     2521 caaacgtctc ggtgaggcgg cccgacacct ccccgtggat cttcgaccgc tcgcggaaga
     2581 tcggccggag ggccccgagg gccgcgaaga gccccgccac gaagaccccg aggaagagga
     2641 gcgtcagccc cgtcaggacc gggctgagcg tcaccatcag gccgagggcg aggagcgagg
     2701 tcaggaggcc tcccgcgagc tcgacgacac ccgtcccgac gaggttccgg atcccttcga
     2761 cgtcgttcat cacccgcgag acgaggttcc ccgtcttctg tccgtcgaag tacgcgacag
     2821 ggagccgcgt cacgtgggac tgcaccctcg tccggagctc cgcgatgagc cgctgcgccc
     2881 ccttcgagag gagctgcgcg agcgcgaacg acgtcgccgc ctggacgagg gtcgccgcgc
     2941 cgatcgcgag gaggagcggc gcgaggaggt cggtgcgaga cttcgcgacg acctcgtcga
     3001 tcagaacttt cgtcgaggcg gggaggacca ggccggccgc ccgcccggcg gccatgagaa
     3061 cgaagcccag ggccaggagc tttcggcgcg gccgagccag ctcgagaagg tcggggacga
     3121 gcgccttcag gcgccggacc ttcggcggct tctctcgacc ctccgcgttc ccgtccggcg
     3181 cggtcatcct ccgacgagct cttcctccgt ctcctcgacg gcgacccctc gggccgcgag
     3241 gtcggcgagg aaccgaccgt agagggcgtc gtctcccgcc aggagctccg gggggaagac
     3301 cccgggcgtg gtgaacgtcc cctccaggag gagccgcgcg ccggtcgcgc aggggaagcc
     3361 ggtcgtccgc gccatcgacg tgtcgcccgt cttcggatcc gtccggtcga ggagctcgaa
     3421 cgtccggcgc acccggcggc cgccgtggcg cccttcgcag acgacgcgca ggaacgtcag
     3481 ctccgcctcg tcctccggcc gccgccacgc gtggaagagg agcgcctccg tcaggcgccg
     3541 cgggacgacc gggactccgt ttaccgccac cggctcgccg gagaagaagc cggtttcgcg
     3601 gagcatcctc atccggtcgg cgtggccggg atagcgaagc gtcttctcgg agaggttccg
     3661 ggccttcacg gtcgagagga gggtccggag cccgtccgtg tagaacgcct cgagcgtccc
     3721 gactccaggg acgtcgagcg gctcgacgcc cgagagggcc gggcggatca cgacctctcc
     3781 cccttccacg cagcgcgacg ggcgcgtgta ctcctcgacg acgtcggtgg gcgagaagac
     3841 agaccggtac tcgaacggcc agtgcctcgc ggcggggagg ccgccgacga agatccggac
     3901 gtcgtcgagg ttgtcgaggc gggcggcggc ccgtcccacg gcgagccccg agaggcccgg
     3961 cgagacgccg cagtcgacga tcgcggggat tcccttcgcc ttcgcgagcg gcgcgaggtc
     4021 gaacgggtct tccggggaga aggagatgtc ggcgaccggc cggcccgtc
//
LOCUS       DAOONL010000231         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_27260_length_3992_cov_15.673355, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000231 DAOONL010000000
VERSION     DAOONL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27260_length_3992_cov_15.673355"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..538)
                     /locus_tag="PLP50_17290"
     CDS             complement(74..538)
                     /locus_tag="PLP50_17290"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPA53350.1"
                     /translation="MKRVVVGKGTQGALLLAYTALVLAGTLLVEGRPGVVTNLVPFDD
                     LARLKASAGKAGVLSNRFVLGLLGLVGNLVMFAIWGFLAWKFVDGRGRARWRNHAEVV
                     FFGLVFSVGIETVQFFLPTRAADVNDVFWNAVGALLGALLAHLHASVRVEWA"
     gene            complement(535..972)
                     /locus_tag="PLP50_17295"
     CDS             complement(535..972)
                     /locus_tag="PLP50_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA53351.1"
                     /translation="MTPLRRLLGCVAVGMLLPGAAGCFGTGTRRVDAPCREMRASVAR
                     EMLRDSREIPLLDVRTMPAPRLRGAHEIPLSALRSRIEEIGRFRRSPLVVVGDDGVAA
                     GQACELLAAEGFLYVIFVPEGAEGLFAGVRGADAVTDGHEERR"
     gene            1143..1808
                     /locus_tag="PLP50_17300"
     CDS             1143..1808
                     /locus_tag="PLP50_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA53352.1"
                     /translation="MKTFPRIALLAVAAAVLAAGPAAAQDRRGTVEITPVVGGYFGGT
                     FDPGTLAFYDGQADASTEVAYGLKLGFNITNNFAIEASYLQSDPKLRLEGGGAIGGGS
                     EEIGEMQMRLYELNFLVPWGRGPVRPYFTIGGGVNTFRPSIPGYSFSSDSRFTGSLGL
                     GVKTYVNPNFGFRFEGKVRSTYVNSEDDYWSCDSWWCDGDYYGESQWYTAGEATAGVI
                     IAF"
     gene            complement(1795..2280)
                     /gene="tsaE"
                     /locus_tag="PLP50_17305"
     CDS             complement(1795..2280)
                     /gene="tsaE"
                     /locus_tag="PLP50_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPA53353.1"
                     /translation="MRTVPFEAVARSVEETREVGRELSLLLVPGSVVRLSGPLGAGKT
                     ELVRGLAEGLGVLPDEVASPTFALVHEYGPEGRPPLLVHVDFYRLLDDERLGAAIEGL
                     GLDETREDGSVVVVEWPEGLPATPGDFEVIVTPERDGARRIEVRRAPVESGSRGGLRR
                     R"
     gene            complement(2277..3782)
                     /locus_tag="PLP50_17310"
     CDS             complement(2277..3782)
                     /locus_tag="PLP50_17310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPA53354.1"
                     /translation="MIALGPAAAREADGRTISAGTPGIVLMERAAEAVARECLRAVDA
                     VPLRGERVVVLCGTGNNGGDGFEAARLLRTSRRVGRVSVLLLGARERVGGDAAETLLR
                     LEQEGGRVVAVDAEAGLEPLRGATMVVDALFGTGLSRPLDAEGLAARAARLASTRRAF
                     VVSVDLPSGLDAGSGALVGPHVRADLTVTFGAPKLAHLLLPAAAFCGRVVVADIGLLP
                     VGSPVVGPEAVTAADVAPLFPPRPASAHKGTYGTLAVIGGAQGMAGAAALAARAAFRA
                     GAGKVVVVAEEASRAALQTLVAEATGAEALRGTGPAALAVGPGLGTGPWGAARLEEAL
                     ATPLPVVLDADALNLLRGRLGEIGARTGPVVLTPHPGEASRLLGLPVSSVAADPANAA
                     LRLAAESRATVVLKGFRSVVATPAGRVARVLAGNPGLATGGSGDVLTGIVGALLARGR
                     TAWEAAAAGAYLHGLAGDLVRERLGEESLRASDLPEELGRAFAAVAESMLP"
     gene            complement(3811..>3992)
                     /locus_tag="PLP50_17315"
     CDS             complement(3811..>3992)
                     /locus_tag="PLP50_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53355.1"
                     /translation="IASKELDALNRRDFVYDVRLLMIPGGQRLSLAVRDEPTNTVSYV
                     QQAIFVSALPQETPR"
BASE COUNT          597 a         1465 c         1464 g          466 t
ORIGIN      
        1 cgggggattt tgagtcccct gcgtctgccg ttccgccact ccggcgcgga acgcggagtc
       61 tagcagcggg ccgtcaggcc cactcgacgc ggaccgaggc gtggaggtgc gccagtagcg
      121 cgccgaggag ggcgccgacg gcgttccaga agacgtcgtt cacgtcggcg gcgcgcgtcg
      181 ggaggaagaa ctgcacggtc tcgatgccga cgctgaagac gaggccgaag aagacgacct
      241 cggcgtggtt tcgccagcgc gcccggcccc gtccgtcgac gaacttccag gccaggaagc
      301 cccagatcgc gaacatgacg aggttgccga cgaggccgag gagcccgagg acgaagcggt
      361 tcgagaggac gccggccttc ccggcgctcg ccttcaggcg cgccaggtcg tcgaacggca
      421 cgaggttcgt gacgacgccg ggccgcccct cgacgagcag cgtcccggcc aggacgaggg
      481 cggtatacgc gaggaggagc gccccctgcg tgcccttccc caccacgacg cgcttcaacg
      541 gcgttcctcg tgcccgtcgg tcacggcgtc ggccccgcgc acgccggcga agaggccttc
      601 ggccccctcc gggacgaaga tgacgtacag gaacccttcg gcggcgagga gctcgcacgc
      661 ctgcccggcg gcgacgccgt cgtctccgac cacgacgagg ggcgatctcc ggaagcgacc
      721 gatctcctcg atgcgggatc gcagcgcgga gagcgggatc tcgtgggccc cccggagccg
      781 aggcgcgggc atcgttcgca cgtccaggag ggggatctcc ctcgagtccc gcagcatctc
      841 gcgagcgacc gacgcgcgca tctcccggca cggtgcgtcg acgcgacgcg ttcccgtgcc
      901 gaaacagccg gcggcgcctg gcaggagcat cccgaccgcg acgcagccga ggagacgtcg
      961 gaggggagtc actcgcgcga gcttaccgga tcgcgacccc gaaaccggac gggacgtgac
     1021 gaagcgcacg tccgcgaacc cgtttcgcga cggtacgtac gttgcagaag gtcagaatga
     1081 aacgttccgc gcgcggccct gcggccgaaa aggaggacgc gggacacctg gaggatcgag
     1141 acatgaagac ttttccgcga atcgccctcc tcgccgttgc ggcggcggtc ctggcggccg
     1201 gcccggccgc cgcgcaggac cggcgcggca cggtggagat cacgccggtc gtcggcggct
     1261 acttcggcgg gacgttcgac cccggaacgc tcgccttcta cgacgggcag gcggacgctt
     1321 cgaccgaggt ggcgtacggc ctgaagctcg gcttcaacat cacgaacaac ttcgcgatcg
     1381 aggcgagcta cctgcagtcg gacccgaagc tccggctcga agggggcgga gcgatcggag
     1441 gcggctccga agagatcggc gagatgcaga tgcggctcta cgagctcaac ttcctcgtcc
     1501 cctggggccg tggaccggtc cgcccgtact tcacgatcgg cggaggcgtg aacacgttcc
     1561 ggccctcgat cccgggctac tcgttctcgt ccgactcccg gttcacgggc agcctcggcc
     1621 tcggcgtgaa gacctacgtg aacccgaact tcggcttccg gttcgagggg aaggtccgtt
     1681 ccacctacgt caacagcgag gacgactact ggtcctgcga cagctggtgg tgcgacggcg
     1741 actactacgg cgaaagccag tggtacaccg ccggagaggc gacggcggga gtcatcatcg
     1801 ccttctgagc ccgcctcggg aaccggattc gacaggggcc cggcggacct cgatccgtcg
     1861 ggccccgtcc cgttctggcg tcacgatgac ctcgaagtcc cccggggtcg ccggaaggcc
     1921 ctccggccac tcgacgacga cgacggaccc gtcttcccgc gtttcgtcga ggccgagccc
     1981 ctcgatcgcc gcgccgagcc gctcgtcgtc gaggagccgg taaaagtcca cgtggacgag
     2041 gagaggcggc cgcccttccg ggccgtattc gtggacgagc gcgaacgtcg gcgaggcgac
     2101 ctcgtccggg aggacgccga gcccctcggc gaggcctcga acgagctcgg tcttcccggc
     2161 gccgagcggc cccgagaggc ggacgacgga tcccgggacg aggagcaggg agagctcccg
     2221 gccgacctcg cgcgtctcct cgaccgagcg cgcgaccgcc tcgaagggga cggtcctcac
     2281 ggcagcatcg actcggcgac ggccgcgaag gcgcggccga gctcctcggg gaggtccgag
     2341 gcccggaggc tctcctcgcc gaggcgctcc cggacgaggt cgcccgccag gccgtgtagg
     2401 taggcccccg ccgcggcggc ctcccacgcc gtccggccgc gcgcgaggag cgctccgacg
     2461 atgccggtca ggacgtcgcc gctccccccc gtcgcgaggc cggggttgcc ggccaggacc
     2521 cgggcgacac gccccgccgg agtcgcgacg acggaccgga atcctttcag gacgaccgtc
     2581 gctcgggact cggcggcgag gcgcagcgcg gcgttcgccg ggtcggccgc gaccgaggac
     2641 acggggagac cgaggaggcg cgaggcctcc ccgggatgag gcgtgaggac gaccggtccc
     2701 gttcgcgcac cgatctcgcc gagccttcct cggagcagat tgagggcgtc ggcgtccaga
     2761 acgacgggga gcggcgtggc gagcgcctcc tcgaggcgcg cggcgcccca gggtcccgtc
     2821 ccgagcccgg ggccgacggc gagagcggcc ggcccggtgc cccgcagggc ctccgcgccg
     2881 gtcgcctccg ccacgagcgt ctggagggcc gcgcggctcg cctcctcggc gacgacgacg
     2941 accttccccg cgccggcccg gaacgccgcc cgggccgcca gggccgccgc ccctgccatc
     3001 ccctgggcgc cgccgatgac agcgagcgtg ccgtacgtcc ccttgtgcgc ggaggcgggg
     3061 cgcggcggga agagcggcgc cacgtcggcc gccgtgacgg cttcgggccc gacgaccggc
     3121 gacccgaccg gcaggagccc gatgtcggcg acgacgacgc ggccgcagaa cgccgccgcc
     3181 ggcaggagga gatgcgcgag cttcggggcg ccgaaggtaa ccgtgaggtc ggcgcggacg
     3241 tgcggcccga cgagcgctcc cgagccggcg tcgagccccg acgggaggtc gacggagacg
     3301 acgaacgccc tgcgagtcga tgcgagcctc gccgcgcgcg cggcgaggcc ctccgcgtcg
     3361 agcggccgcg acaggcccgt cccgaagagg gcgtcgacga ccatcgtcgc gccgcgcagc
     3421 ggctcgagac cggcctccgc gtccaccgcc acgacccggc ccccctcctg ctcgaggcgg
     3481 aggagcgtct cggccgcgtc gcccccgacg cgctcccgcg ccccgaggag gagcacggag
     3541 acccgaccga cgcgacggct cgtccggagg agccgcgccg cctcgaagcc gtcccctccg
     3601 ttgttccccg tcccgcagag gacgacgacg cgctcgccgc ggagagggac cgcgtcgacg
     3661 gcccgcaggc actcccgggc gacggcctcg gccgcccgct ccatgaggac gatcccgggc
     3721 gtgccagccg agatcgtccg gccgtccgct tcgcgcgcgg cagccggccc cagggcgatc
     3781 acgcccgctc cggccgccgc gccgcggcga tcaccggggc gtctcctgcg ggagggcgga
     3841 gacgaagatc gcttgctgga cgtagctgac ggtgttcgtc ggctcgtcgc ggaccgcgag
     3901 cgagaggcgc tggccaccgg ggatcatcag gagcctcacg tcgtagacga agtcgcgccg
     3961 gttgagcgcg tcgagctcct tcgaggcgat ct
//
LOCUS       DAOONL010000232         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_27657_length_3948_cov_13.083997, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000232 DAOONL010000000
VERSION     DAOONL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27657_length_3948_cov_13.083997"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLP50_17320"
     CDS             <1..250
                     /locus_tag="PLP50_17320"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPA53356.1"
                     /translation="GVYANFVNIIHNPAEFVFDFARAVPGRNDVRILSRVLTTPVHAK
                     QLLNALAQNIAVYEKSYGPIRVDFEAPRPGAGAPVRPN"
     gene            275..766
                     /locus_tag="PLP50_17325"
     CDS             275..766
                     /locus_tag="PLP50_17325"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA53357.1"
                     /translation="MTPTTRLAAVFLLAAAPLALAAEPPAPEGLPIYDTTSVGALAID
                     AASKAATESGRRLLVNFGTNDCAPCRVVNDAIYEEPFLSAFFRQFIPVFVDVAPGSPN
                     RALLERYGIDPAKGFPALVLSDDSLKVAEVTKGGEVAALGAKGKDEVRAWLLKRFKAD
                     APK"
     gene            complement(863..1570)
                     /locus_tag="PLP50_17330"
     CDS             complement(863..1570)
                     /locus_tag="PLP50_17330"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPA53358.1"
                     /translation="MDEKARSVPVAKGALKVIGVRPGSVKAVAREIAVRHKATLAYPL
                     VAELLDAAASRKVREEILVAVELLDRFRKEFEPALLVRMDKWSSVADDLEVAEALGEK
                     VAAFVLALDPSKITTIRKWARLRSVGRKRLALLAAAGLVSEGRRDAALALDLCEILLN
                     EDHPVLVREVASLLRTTTKIDAKAVQDFLFRRSIDGNPAILRAGSENLDAARRAALIA
                     KLEAQQASLGALAAAGR"
     gene            complement(1735..2925)
                     /gene="mnmA"
                     /locus_tag="PLP50_17335"
     CDS             complement(1735..2925)
                     /gene="mnmA"
                     /locus_tag="PLP50_17335"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPA53359.1"
                     /translation="MRIAVAMSGGVDSSVAALLLAREGAEVIGVSMHLWDHDREGTGA
                     GEGRCCTLDDLRTARRAAEAIGVPHYTLDLREKFLDAVVRPFVASYLAGETPVPCLAC
                     NTEVKFDALFRKARALGCEAVATGHYARLERDERTGELRLLKARDAAKDQTYFLYELT
                     PEQLAAARFPLGDLTKAEVRELAREAGLPNWEKPDSQEICFVSAEDGPAGFIRKEAAS
                     LGFALPSHPAALPGDLVRADGTPLGAHPGTLGFTVGQRRGLGVAAGEPLYVVGIRVED
                     RQLVVGSGEELLSRVVFLRDVNLQVPRAGTSYPVLSRIRSRHPDVPARLELPDGESRR
                     GGRARLVFEEPVRAAAPGQSAVFYDPDRPELLLGGGRIDRAVSPGGTGGETGAQGCAK
                     GTTV"
     gene            2974..3273
                     /locus_tag="PLP50_17340"
     CDS             2974..3273
                     /locus_tag="PLP50_17340"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPA53360.1"
                     /translation="MPRPPQLSPGEITDRLASLPGWTLTDGALRREFVFADFPEAFAF
                     MTRVALVAEKMDHHPEWTNVWSRVSVRLTTHDAGGVTALDLELAGVMHRLMRNGD"
     gene            3320..>3948
                     /locus_tag="PLP50_17345"
     CDS             3320..>3948
                     /locus_tag="PLP50_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010278923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPA53361.1"
                     /translation="MEAPDSHEDLLELLSKSGKAVFAIDSSDRIVLWNTGAERLLGYP
                     AADVLGRSCHQAMSGRDVYGNRYCFGECPILRMRDRGETIQPFEIVLESRDAGPKRLR
                     CSILSIPGQRPELTTLVHILAETNGAHPDPGGFFQRFSGPVQHPSLAPLVPPRPASSP
                     LTKREQEVLRLLSEGKKTDEMGEQLFISDVTVRNHIQNILRKLNVHSKL"
BASE COUNT          580 a         1492 c         1315 g          561 t
ORIGIN      
        1 cggcgtctac gcgaacttcg tcaacatcat ccacaacccg gccgagttcg tcttcgactt
       61 cgcccgggcc gttcccgggc gcaacgacgt gcggatcctc tcgcgggtcc tcaccacgcc
      121 cgtccacgcc aagcagctcc tcaacgccct ggcgcagaac atcgccgtct acgagaagag
      181 ctacggcccg atccgcgtcg acttcgaggc tccgcgcccc ggagccgggg caccggtccg
      241 accgaactga acacccgcgc acggaaaggg aacgatgaca ccgaccaccc gcctcgccgc
      301 cgtcttcctc ctcgccgccg cgccgctggc cctcgccgcc gagccgcctg cgccggaggg
      361 cctcccgatc tacgacacga cgagcgtcgg cgcgctggcg atcgacgccg cctcgaaggc
      421 cgccaccgag tccggccggc ggctgctcgt gaatttcggc accaacgact gcgcgccgtg
      481 ccgcgtcgtg aacgacgcga tctacgagga gcccttcctt tcggcgttct tcagacagtt
      541 catccccgtc ttcgtcgacg tcgcgccggg ctcgccgaac cgggccctcc tcgagcgcta
      601 cgggatcgac ccggcgaagg ggtttccggc gctcgtcctt tccgacgact cgctcaaggt
      661 cgcggaggta acgaagggcg gcgaggtcgc ggcgctcggc gcgaagggga aggacgaagt
      721 ccgcgcctgg ctcctgaagc gattcaaggc cgacgcaccg aagtagtcgt cccctcggac
      781 cgggaggcgg cgccgccggg cgcctcctcc cggccggagc ggcccgccgg gagcgcgctc
      841 ggagggaggg aggccgcggg gctcagcgcc cggcggcggc cagcgcgccg agactcgcct
      901 gctgcgcctc gagcttcgcg atgagcgcgg cgcggcgagc ggcgtcgagg ttctccgagc
      961 cggcccggag gatcgcgggg ttcccgtcga tcgagcgccg gaacaggaag tcctggacgg
     1021 ccttcgcgtc gatcttcgtc gtcgtgcgca ggaggctcgc gacctcccgc accaggaccg
     1081 ggtggtcctc gttcaggagg atctcgcaga ggtcgagcgc cagcgccgcg tcgcgcctcc
     1141 cctcggagac gaggcccgcg gcggccagga gcgcgaggcg cttccgcccg acgctgcgga
     1201 gccgggccca cttgcggatc gtcgtgatct tcgacgggtc gagcgcgagg acgaaggccg
     1261 ccaccttctc acccaacgcc tcggcgacct cgaggtcgtc ggcgacggag ctccacttgt
     1321 ccatccggac gaggagcgcg ggctcgaact ccttgcggaa cctgtcgagg agctccaccg
     1381 cgacgaggat ctcctcgcgc accttccggg aggcggcggc gtcgagcagc tcggcgacga
     1441 gcggataggc gagcgtcgcc ttgtgcctga ccgcgatctc ccgcgccacg gccttgacgc
     1501 tgccgggacg gacgccgatc accttcagcg cccccttggc gacgggaacc gagcgcgcct
     1561 tctcgtccac tctgtcgtcg agagcggctc gaatgtcctt taggagggag gcgacgtgcg
     1621 gggaagcggt cttggccata cgcggacgac gcgagagttt atcacgtcgg cgagcgctcg
     1681 gacacctcac gcacggcccg cgcgccgggc ggccaccacc ttccccggcc gatgctacac
     1741 cgtcgtcccc ttcgcgcaac cctgagcgcc ggtctcgcct cccgtccccc cgggcgacac
     1801 ggcacggtcg atccggcctc cgccgagcag gagctcgggc cggtccggat cgtagaagac
     1861 cgccgactgg cctggcgcgg cggcgcgaac cggctcctcg aacaccagtc gggcccgccc
     1921 cccccgccgg ctctccccgt ccggcagctc gagccgtgcc ggaacgtccg ggtgccggga
     1981 ccggatccgc gagaggaccg ggtacgacgt gccggcgcgc gggacctgca ggttcacgtc
     2041 ccggaggaag accacccgcg agaggagctc ttcgccgctc ccgacgacga gctgccggtc
     2101 ctcgacgcga atcccgacga cgtagagggg ctctccggcg gccaccccca gcccccggcg
     2161 ctgccccacc gtgaagccga gcgttcccgg atgggctccg aggggcgtgc cgtccgccct
     2221 cacgaggtcg ccggggagcg ccgccggatg gctcggaagc gcgaagccga ggctcgccgc
     2281 ctccttccgg atgaagcccg ccggcccgtc ctcggcggag acgaagcaga tctcctgaga
     2341 gtcgggcttc tcccagttcg gcagtcccgc ctcgcgcgcg agctcgcgga cctcggcctt
     2401 cgtcaggtcg ccgagcggga atcgggccgc ggcgagctgc tcgggcgtca gctcgtagag
     2461 gaagtaggtc tggtctttcg cggcgtcgcg ggccttgaga agccggagct ctcccgtcct
     2521 ctcgtcccgc tcgaggcggg cgtagtgtcc cgtggccacc gcctcgcatc cgagcgcccg
     2581 cgccttccgg aacagggcgt cgaacttcac ctccgtgttg caggcgaggc acgggacggg
     2641 cgtctcgccg gccaggtacg acgccacgaa cgggcggacg accgcgtcga ggaacttctc
     2701 ccgcaggtcg agcgtgtagt gcggcacgcc gatcgcctcg gcggcccggc gcgccgtgcg
     2761 gaggtcgtcg agggtgcagc agcgtccctc gcccgccccg gtcccctccc ggtcgtgatc
     2821 ccagaggtgc atcgagaccc cgatcacctc cgccccttcc ctcgcgagga ggagcgccgc
     2881 gaccgaggaa tcgactccac ccgacatggc gaccgcgatc ctcatagact ccgcagttta
     2941 cgtgccgggg ccacgcgccc cgtggaggtg ccgatgcccc gcccccccca gctctccccc
     3001 ggcgagatca ccgaccgcct cgcctcgctt cccggctgga ctctcaccga cggcgcgctc
     3061 cgccgcgagt tcgtcttcgc cgacttcccg gaggcgttcg cgttcatgac ccgggtcgcc
     3121 ctggtggccg agaagatgga ccatcacccc gagtggacga acgtctggtc gcgcgtctcg
     3181 gtcaggctca cgacgcacga cgcaggaggc gtcacggcgc tcgatctcga gctggccgga
     3241 gtgatgcatc gtctgatgag aaatggggat tgagactggt gcattcgcat cagctccgag
     3301 ggtagaatcc gcccccggaa tggaagctcc cgacagccac gaggacctcc tcgagctcct
     3361 ctccaagagc ggcaaggccg tcttcgcgat cgactcctcg gaccggatcg tcctctggaa
     3421 caccggtgcc gagaggctcc tcggctaccc cgccgccgac gtcctcgggc ggagctgtca
     3481 tcaggcgatg tcgggccggg acgtctacgg caatcgctac tgcttcgggg agtgcccgat
     3541 cctccggatg cgcgaccggg gcgagaccat ccagcccttc gagatcgtcc tcgagtcccg
     3601 cgacgccggg ccgaagcgac tccggtgctc gatcctctcg attcccggtc agcgcccgga
     3661 gctgacgacg ctcgtccaca tcctcgccga gacgaacgga gcccatcccg acccgggagg
     3721 gttcttccag cgcttctccg gtcccgtcca gcacccgagc ctcgcgccgc tcgtcccgcc
     3781 ccgcccggcc tcctctccgc tgaccaagcg tgagcaggag gtcctccggc tgctctccga
     3841 ggggaagaag accgacgaga tgggcgagca gctcttcatc agcgacgtca ccgtgaggaa
     3901 ccacatccag aacatcctcc ggaagctgaa cgtccactcg aagctcga
//
LOCUS       DAOONL010000233         3900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_28124_length_3900_cov_12.279584, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000233 DAOONL010000000
VERSION     DAOONL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28124_length_3900_cov_12.279584"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..812)
                     /locus_tag="PLP50_17350"
     CDS             complement(216..812)
                     /locus_tag="PLP50_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPA53362.1"
                     /translation="MQITDILAQMGGLQSMARELGISEAQAAKGAEALAPAILGGFKK
                     QAQVQPTGLEGLGGLLGQLGGGGLLDEVLSPQPTNVSHGNDVLGQIFGSKDVSRAVAQ
                     NASAKSGLDPSILKKMLPMLAMLVTGFMAKQGGGGGAQASAGGGLGDLLGGLMGGGAN
                     RSAAPAAGASGGLGSLLDLDGDGNALDDILGMAGKLMR"
     gene            complement(848..1261)
                     /locus_tag="PLP50_17355"
     CDS             complement(848..1261)
                     /locus_tag="PLP50_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HPA53363.1"
                     /translation="MGLFGSILAKLGIGKPEPAPAPAPAPAPRPVPPVAAPAPTPAEP
                     PAPAPIPVVDVVAQLEQRAAANPQKLNWKTSIVDLLKLLDIDSSFAARKELATELGCP
                     AELMGDSAKMNVWLHKTVLAKIAENGGNVPKELLD"
     gene            1499..3646
                     /locus_tag="PLP50_17360"
     CDS             1499..3646
                     /locus_tag="PLP50_17360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPA53364.1"
                     /translation="MRRRIVALAAVAMALPGLAQTPAEPKAETNPFFMEWATPFGMAP
                     FEKIRAEHFLPAFEAGIAERRKEVEAIVSNAAAPTFANTVEALENGGAALSKVSNVFY
                     TLVGAETNDELQAITKKVAPSLSALRDDEMLNEKLFARVKAVWEKRETSSLTPEQKRL
                     VDDTYKGFVRGGANLAPEARKRFREINQELSLLGIRFGDNLLKETNGFRLVIEKKADL
                     AGLPPAVVAAAADAATAAKLDGKWVFTLQYPSIWPFMTFSENRELRRQLLTAYSTRCD
                     KGGETDNKEILAKMAALRAERAKLLGFATWADFVLDDNMAKTPARVYELLDRVWKPAL
                     EVAKADAKELEKALQADVPGGKLEPWDWHFYAEKVRKARFDLDDQELRPFFPLDRVRE
                     GAFSVANRLYGITFTERKDVPKYHAEVSTYEVKDADGSFLALYTTDYHPRAGKRGGAW
                     CGRLRDQWVRDGKDVRPIVTNVMNFTRPSGDAPALLSLDEVETLFHEFGHALHRILSR
                     VTYRSMAGVPRDFVELPSQIMENWALEPTVLAGYAKHWKTNAPIPAALVEKIEKSSLY
                     GQGFATVEYTAASYLDMNWHTLAEPKVEDATAFEKAALAKIGLIPEILPRYRSPYFSH
                     IFGGGGGYSAGYYSYLWSEVLDSDAFDAFKEKGIFDPATATSFRRNVLEKGGTDDAMA
                     LYRAFRGREPSVEPLLVKRGLAPAVKPGTPASK"
     gene            complement(3691..>3900)
                     /locus_tag="PLP50_17365"
     CDS             complement(3691..>3900)
                     /locus_tag="PLP50_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53365.1"
                     /translation="IEIDTRRIAVFAHRAGLEIGSVFHETAVRLAYLWEPVEALVNTL
                     LMRETVSGALAARIIRATHPGPALE"
BASE COUNT          594 a         1375 c         1372 g          559 t
ORIGIN      
        1 ggacgtcacc ctttcaaggt gaagatcgcg ggttcgaatc ccgctggggg taccaacctc
       61 ttcctgccgt cgagacgggc gtccctcgct ctcgcgccca cgaacgacac gcggcgagcc
      121 ggcgcgctcc tccccgttcg cgcgaagagg ggcgccggcc cgttcgaccg gtcgtgtcgc
      181 gcgggagacc cgcgtcgcgg gtctcccgac gggattcagc gcatcagctt cccggccatt
      241 ccgaggatgt cgtcgagggc gttcccgtcc ccgtcgaggt cgaggagcga gccgaggccg
      301 cccgaggcgc cggcggccgg cgcggcggag cggttcgcgc cgccccccat gagcccgccg
      361 aggaggtcgc cgaggccgcc gcccgccgag gcctgcgcgc ctcccccgcc cccctgcttc
      421 gccatgaagc cggtgacgag catcgcgagc atcgggagca tcttcttgag gatcgacggg
      481 tcgaggcccg acttcgccga cgcgttctgc gcgacggcgc ggctcacgtc cttggagccg
      541 aagatctggc cgaggacgtc gttgccgtgg ctcacgttcg tcggctgtgg cgagaggacc
      601 tcgtcgagga ggccgccgcc gccgagctgg ccgaggaggc cgccgagccc ttcgagaccc
      661 gtcggctgca cctgagcctg cttcttgaac ccaccgagga tcgccggcgc gagggcctcg
      721 gcccccttcg cggcctgcgc ctcgctgatg ccgagctcgc gggccatcga ctggagaccg
      781 cccatctggg cgaggatgtc ggtgatctgc atacgctgtc tcctctcttc cgtcgggcgc
      841 cccgcgctca gtcgaggagc tccttcggga cgttgccgcc gttctcggcg atcttcgcga
      901 ggacggtctt gtgcagccag acgttcatct tcgccgagtc gcccatgagc tccgcggggc
      961 agccgagctc ggtggcgagc tccttgcggg cggcgaagct gctgtcgatg tcgaggagct
     1021 tgaggaggtc gacgatcgag gtcttccagt tgagcttctg ggggttcgcg gccgcccgct
     1081 gctcgagctg ggcgacgacg tcgacgaccg ggatcggcgc gggagccggg ggctccgcgg
     1141 gcgtcggggc cggggccgcg acgggaggaa cggggcgcgg tgccggcgcc ggggcgggag
     1201 ccggggccgg ctcgggcttg ccgatgccga gcttggcgag aatcgatccg aaaagtccca
     1261 tgacgtcctc ctggtctgct ttgtgcggcg acggaccctg ccggaaggac agagagcgga
     1321 gccgcagttc cgtccggtca cggtactggc gcgacccgtc gaaatcaatg aggacgttcg
     1381 cggctccggc cgggaccggc tcttcgggcg gctgcgcctc gcgcgaccgc gtaagatgca
     1441 gggtccgccc ggcgcggggc ctcgaggccc tccgcgccgg gactcaggga ggaatcgcat
     1501 gagaagacgc atcgttgccc tcgcggccgt cgcgatggcg ctgccgggtc tcgcccagac
     1561 cccggccgaa ccgaaggccg agacgaaccc gttcttcatg gagtgggcga cgccgttcgg
     1621 catggctccc ttcgagaaga tccgcgcgga gcacttcctt ccggccttcg aggcggggat
     1681 cgccgagcgt cgcaaggaag tcgaggcgat cgtgtcgaac gccgccgcgc cgacgttcgc
     1741 gaacaccgtc gaagcgctcg agaacggcgg cgccgccctt tcgaaggtca gcaacgtctt
     1801 ctacacgctc gtcggggcgg agacgaacga cgagctccag gcgatcacga agaaggtcgc
     1861 cccctccctg tcggccctcc gggacgacga gatgctcaac gagaagctct tcgcccgcgt
     1921 gaaggcggtc tgggagaagc gggagacgtc gagcctgacg ccggagcaga agcggctcgt
     1981 cgacgacacc tacaagggct tcgtccgcgg cggcgcgaac ctcgcgcccg aggcgcggaa
     2041 gcggttccgc gagatcaacc aggagctctc gcttctcggc atccgcttcg gcgacaacct
     2101 cctgaaggag acgaacggct tccggctcgt gatcgagaag aaggccgacc tcgccgggct
     2161 cccgcccgcc gtcgtcgccg cggcggccga cgccgcgacg gccgcgaagc tggacgggaa
     2221 gtgggtcttc acgctccagt acccctccat ctggccgttc atgaccttct ccgagaaccg
     2281 ggagctccgc cggcagctcc tgacggccta ctcgacccgc tgcgacaagg ggggcgagac
     2341 ggacaacaag gagatcctcg cgaagatggc ggcgctccgc gccgagcggg cgaagctcct
     2401 cggcttcgcg acctgggccg acttcgtcct cgacgacaac atggcgaaga cgcccgcgcg
     2461 tgtctacgag ctgctggacc gggtctggaa gccggccctc gaggtggcga aggccgacgc
     2521 gaaggagctc gagaaggccc ttcaggccga cgtgccgggc gggaagctcg agccgtggga
     2581 ctggcacttc tacgccgaga aggtccgcaa ggcgcggttc gacctggacg accaggagct
     2641 ccgccccttc ttcccgctcg accgcgtccg cgaaggggcc ttctcggtcg cgaaccggct
     2701 ctacggcatc acgttcaccg agcggaagga cgtgccgaag taccacgccg aggtgagcac
     2761 gtacgaggtg aaggacgccg acgggtcgtt cctcgccctc tacacgacgg actaccaccc
     2821 gcgcgccggc aagcgcggcg gagcctggtg cggccgcctg cgcgaccagt gggtgaggga
     2881 cggcaaggac gtccgcccga tcgtcacgaa cgtcatgaac ttcacgcgcc cctccggcga
     2941 cgcgccggcg ctcctctcgc tcgacgaggt ggagacgctc ttccacgagt tcggccacgc
     3001 gctccaccgg atcctctcgc gcgtcaccta ccgctcgatg gcgggcgttc cgcgcgactt
     3061 cgtggagctt ccgtcccaga tcatggagaa ctgggctctc gagccgacgg tcctcgcggg
     3121 ctacgcgaag cactggaaga cgaacgcgcc gatcccggcc gcgctcgtcg agaagatcga
     3181 gaagtcgagc ctctacggac agggcttcgc gacggtcgag tacaccgccg cctcgtacct
     3241 cgacatgaac tggcacacgc tcgccgagcc gaaggtcgag gacgcgaccg cgttcgagaa
     3301 ggcggccctc gcgaagatcg ggctcatccc cgagatcctc ccccgctacc ggagccccta
     3361 cttcagccac atcttcggcg ggggcggcgg ctactccgcc ggctactaca gctacctctg
     3421 gagcgaggtc ctcgactccg acgccttcga cgcgttcaag gagaagggga tcttcgaccc
     3481 ggcgacggcg acctcgttcc gccgcaacgt cctcgagaag ggcgggaccg acgacgcgat
     3541 ggcgctctac cgggcgttcc gcgggcgcga gccctcggtc gagcctctcc tcgtcaagcg
     3601 cggcctcgcg ccggccgtga agcccgggac gcccgcctcg aagtaggtcc gcgcagcggg
     3661 gagagggccc gagccctctc cccgcccgcg ctactcgagc gcgggcccgg gatgcgtcgc
     3721 gcggatgatc cgcgcggcga gcgcgccgga gaccgtctcc cgcatcagga gcgtgttcac
     3781 gagcgcctcg accggctccc agaggtaggc gaggcgcacg gccgtctcgt ggaagaccga
     3841 gccgatctcg agccccgcgc ggtgcgcgaa gacggcgatg cggcgggtgt cgatctcgat
//
LOCUS       DAOONL010000234         3895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_28175_length_3895_cov_14.017708, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000234 DAOONL010000000
VERSION     DAOONL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28175_length_3895_cov_14.017708"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="fusA"
                     /locus_tag="PLP50_17370"
     CDS             complement(<1..1817)
                     /gene="fusA"
                     /locus_tag="PLP50_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPA53366.1"
                     /translation="MARTHALENCRNIGIMAHIDAGKTTTTERILFYTGVNYKLGEVH
                     EGTATMDWMVQEQERGITITSAATTCAWRGTRINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFDAVSGVEPQSETVWRQADRYGVPRIAFVNKMDRVGADFERCVEMMIAKLGAKPV
                     PVILPWGSAETFRGVLDVLEEKAYDFRDEALGADFETVEVPAEHRADLAKWREKAIEA
                     IAETDDELMHKYLEGTVPSADELKAALRRATIVGRLHPVVCGSAFKNKAVQQLLDSVV
                     AYLPSPVDIPPIKGIDEDGKEVSRETKDDAPFSGLVFKIMTDPFVGQLAFFRVYSGHL
                     DAGSAVYNATKQTNERVGRLLKMHANKREEIKQVWAGDIAAAVGLKNVVTGDTICEKG
                     NVVVLEAMKFPEPVIELSIEPKTKADQEKMGLALAKLMQEDPTFRVSTDKETNQTIIA
                     GMGELHLEIIVDRLIREFNVGANVGKPQVAYRESIRKEAQAEGRFVRQTGGKGQYGHA
                     KIRLRPHPEGKEYEFVNAIVGGVIPREFIKPIDQGIREAILTGVLAGYPTTQIQVELY
                     DGSYHDVDSSEMAFKIAGSMAFQAAAKKADPVILEPMMAV"
     gene            complement(2037..2507)
                     /gene="rpsG"
                     /locus_tag="PLP50_17375"
     CDS             complement(2037..2507)
                     /gene="rpsG"
                     /locus_tag="PLP50_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPA53367.1"
                     /translation="MPRRREVPKREVLPDPLYQSQLVTKFISSVMKCGKKSTAEGIFY
                     GAMKQIEGKTQDDPLKTFKKALENVKPVLEVKSRRVGGSTYQVPVEVKPNRRMSLAIR
                     WIINYSQARGEKTMQDKLAGELLDAANNRGNSVKKREDTHKMAEANKAFAHYRW"
     gene            complement(2525..2899)
                     /gene="rpsL"
                     /locus_tag="PLP50_17380"
     CDS             complement(2525..2899)
                     /gene="rpsL"
                     /locus_tag="PLP50_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPA53368.1"
                     /translation="MPTISQLVRKGREAVRYKTKSPALQSCPQRRGVCTQVKTTTPKK
                     PNSALRKIARVRLTNGIEVTTYIPGIGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DAVGVANRKQSRSKYGAKRPKS"
     gene            complement(3332..>3895)
                     /locus_tag="PLP50_17385"
     CDS             complement(3332..>3895)
                     /locus_tag="PLP50_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPA53369.1"
                     /translation="PHDILAVKGEKELQRYMTDKIQEVYRSQGVGINDKHIEVIVRQM
                     MRSVRVEEVGDTEFLIDEQVDKFRFREENDRIVAAGGKPAQGRPLLLGITKASLSTDS
                     FVSAASFQETTRVLTEASIAGRVDLLRGLKENVIVGRLIPAGTGMEFYRNVELEREEI
                     PVLPDEIEEPFYDDEPPVVAADLDEEI"
BASE COUNT          539 a         1285 c         1316 g          755 t
ORIGIN      
        1 acggccatca tcggctcgag gatgaccggg tcggccttct tcgccgccgc ctggaacgcc
       61 atcgagccgg cgatcttgaa ggccatctcc gacgagtcga cgtcgtggta cgagccgtcg
      121 tagagctcga cctggatctg cgtcgtcggg tatccggcga ggacgccggt gaggatcgcc
      181 tcgcggatcc cctggtcgat cggcttgatg aactccctcg ggatgacgcc gccgacgatc
      241 gcgttgacga actcgtactc cttcccctcg gggtggggac ggaggcggat cttcgcgtgg
      301 ccgtactgcc ccttgccgcc cgtctggcgg acgaagcgtc cttcggcctg ggcttccttc
      361 cggatcgact cgcggtaggc gacctggggc ttgccgacgt tcgcgccgac gttgaactcg
      421 cggatgaggc ggtcgacgat gatctcgagg tggagctcgc ccatcccggc gatgatcgtc
      481 tggttcgtct ccttgtccgt cgagacccgg aacgtcgggt cttcctgcat gagcttcgcg
      541 agggcgaggc ccatcttctc ctggtcggcc ttcgtcttcg gctcgatgga gagctcgatg
      601 accggctcgg ggaacttcat cgcctcgagg acgacgacgt tgcccttctc gcagatcgtg
      661 tcacccgtga cgacgttctt cagcccgacg gcggcggcga tgtcgcccgc ccagacctgc
      721 ttgatctcct cacgcttgtt cgcgtgcatc ttgaggagcc ggccgacgcg ctcgttcgtc
      781 tgcttcgtgg cgttgtagac ggcggagccg gcgtcgagat ggcccgagta gacccggaag
      841 aaggcgagct ggccgacgaa cgggtcggtc atgatcttga agacgaggcc cgagaagggc
      901 gcgtcgtcct tcgtctcgcg ggagacttcc ttcccgtcct cgtcgatccc cttgatcggc
      961 gggatgtcca ccggggacgg gaggtaggcg acgacggagt cgaggagctg ctggacggcc
     1021 ttgttcttga aggccgagcc gcagacgacc gggtggagcc tgccgacgat cgtggcccgc
     1081 cgcagcgcgg ccttcagctc gtcggccgag gggaccgtcc cctcgaggta cttgtgcatg
     1141 agctcgtcgt cggtctcggc gatcgcctcg atcgccttct cgcgccactt cgcgaggtcg
     1201 gcgcggtgct cggccggcac ctcgacggtc tcgaagtccg ccccgagagc ctcgtcgcgg
     1261 aagtcgtagg ccttctcctc gaggacgtcc aggacgccgc ggaacgtctc cgcggagccc
     1321 caggggagga tgaccggaac gggcttcgct ccgagcttgg cgatcatcat ctcgacgcaa
     1381 cgctcgaagt cggccccgac ccggtccatc ttgttgacga acgcgatgcg gggaactccg
     1441 tagcggtcgg cctggcgcca gacggtttcg gactgcggct cgacgcccga gacggcgtcg
     1501 aagacggcga cggcgccgtc gaggacccgg agcgagcgct ccacctcggc cgtgaagtcg
     1561 acgtgccccg gcgtgtcgat gatgttgatc cgggtgcctc gccacgcgca ggtggtcgcg
     1621 gccgaggtga tcgtgatgcc gcgctcctgc tcctggacca tccagtccat ggtggccgtg
     1681 ccctcgtgga cctctcccag cttgtagttg acgccggtgt agaagaggat cctctcggta
     1741 gtcgtcgtct taccggcatc gatgtgagcc atgatgccga tgttccggca gttctcgagc
     1801 gcgtgcgttc tcgccataaa ggttgtgttc ccgacctcgg tcgtgtctgc gccgtcgaac
     1861 ccttccggcc ttcgcgtctc cgcgggctcg gacgggttcc gcacgctgtt aggcgtggcc
     1921 cggtgcggtt gtttccgcgg acctcgtcgt cccctggggt acgaccggtt tccacggctt
     1981 ccgggcttcc cggggagacc gccgtcgacc ggcgccctcc cttaccgtgc aggcaactac
     2041 cagcggtagt gcgcgaaggc tttattggcc tccgccatct tgtgggtgtc ctccctcttc
     2101 ttcacggagt tgccgcggtt gttcgccgcg tccaggagct ctccggcgag cttgtcctgc
     2161 atggtcttct cgccgcgggc ctgcgagtag ttgatgatcc agcggatcgc cagggacatc
     2221 cggcggttcg gcttcacctc gaccggcacc tggtaggtcg agccgccgac gcgacgggac
     2281 ttgacctcga ggacaggctt gacgttctcc agggccttct tgaacgtctt gagcgggtcg
     2341 tcctgggtct tcccctcgat ctgcttcata gcgccgtaga agatgccctc cgcggtggac
     2401 ttcttcccgc acttcatgac ggagctgatg aacttggtga cgagctggga ctggtagagc
     2461 gggtccggaa ggacctctcg cttcggaact tcgcgacggc gcggcacggc ttctctcccc
     2521 tacctcagga cttcgggcgc ttggcgccgt acttggagcg ggactgcttc ctgttggcga
     2581 cgccgaccgc gtcgagcgtc ccacgaatga tgtggtagcg aacgccggga aggtccttca
     2641 cgcgcccgcc acggatcatg acgatggagt gctcctggag gttgtgcccg ataccgggga
     2701 tgtaggtcgt gacctcgatg ccgttcgtca gccggacgcg ggcgatcttg cggagcgccg
     2761 agttcggctt cttgggggtc gtggtcttga cctgggtgca gaccccccgc cgctgcgggc
     2821 aggactggag ggcaggcgac ttcgtcttgt aacggacggc ttcgcggccc ttacgaacga
     2881 gctggctgat cgtcggcatc agacttcctc ggttcctctt ccccgccgga gagacgactt
     2941 ttcggccctt ggggccgctc tctccccgga tttttcgatg tcggatcgct ctcccggaag
     3001 gccggaacgg ctctgtccga ggggcgaaac cggcagagac tacgtgtaag tcccccgcat
     3061 gtcaacccgg tacgtgcacc ggacgccatc ggggccgtat gggaacccgg cggaccgggt
     3121 ccccccttac tcagcgcgag cgcggggctt cgaagtacca cggcccgcca tgtgagggcc
     3181 gggggtccgg ggggccgagc gaagctcagg tcccccggcg agcgcgagct gccttcccgc
     3241 gcggaggggt tcggggaccg atgggagcgc cggtcaggcg ctcccatcga aggcgaagcc
     3301 aacccggcgg tccgggttcc ccgagaacat gtcagatctc ttcgtcgagg tccgccgcca
     3361 cgaccggcgg ctcgtcgtcg tagaacggct cctcgatctc gtcgggcagg acggggatct
     3421 cctcccgctc cagctcgacg ttccgataga actccatgcc ggtcccggcc gggatgaggc
     3481 gcccgacgat gacgttctcc ttcaggccgc ggaggaggtc cacccggccc gcgatcgacg
     3541 cctccgtgag gacccgcgtc gtctcctgga acgaggcggc cgagacgaac gagtcggtcg
     3601 agagggaggc cttcgtgatc ccgaggagga gcggacgccc ctgggccggc ttgccgcccg
     3661 ccgcgacgat ccggtcgttc tcctcgcgga agcggaactt gtcgacctgc tcgtcgatca
     3721 ggaactcggt gtcgccgacc tcctcgacgc ggacgctccg catcatctgg cggacgatga
     3781 cctcgatgtg cttgtcgttg atgccgacgc cctgcgagcg gtagacctcc tggatcttgt
     3841 cggtcatgta ccgctggagc tccttctcgc ccttgaccgc gaggatgtcg tgcgg
//
LOCUS       DAOONL010000235         3886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_28262_length_3886_cov_18.589402, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000235 DAOONL010000000
VERSION     DAOONL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28262_length_3886_cov_18.589402"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLP50_17390"
     CDS             <1..1134
                     /locus_tag="PLP50_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPA53370.1"
                     /translation="RVMALTIPQWIGTRWESKGMRLAFVVLSAGLLAFATLIVVALAH
                     VLAGLLGVPAVAVAAAVLLYVLLAMAMGGATAHAWINAFQAIVMLVVAVLLVGTAVGQ
                     AAPFGGVLERLGAIQPSLATVVNPASPYFRNLFEVFFCNFLVGLAIVGQPHILSKALY
                     LSDGRQVKRYLGVAIGAGLVFMGVLWVGLAARLSLPALPRIDLVVPSWIGASFGPGLS
                     VVVAVGIICAGLSTLEGIFLALASILSVDVHPLLPGAKPERALRFGRIGLAVVAVVCL
                     LLARWQIAHPTGGSVAIFAQYGIYLLFTASSVPLVAGMFLPRARRGAVALAATAAVVV
                     YVVAGALKLGALSNNPAVLATWGLLASWAVLGASLLLAGRREA"
     gene            1131..2498
                     /locus_tag="PLP50_17395"
     CDS             1131..2498
                     /locus_tag="PLP50_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HPA53371.1"
                     /translation="MSSPDAPRSGAVASLMRPLNTLVERFLPSALVFAVVLTGIVAVL
                     ALLLTDAGPAEVLRGWGDGLSGLLAFTTQMALILMLGHVLASTRPVRALLARLGSLPR
                     TPRQAYVSVFLVAALSSLVTWGLGLVVGALLAREVAAQGRARGLRLHFPLLVAAGFSG
                     FVVWHMGSSGSGPLTAATEGSFIAAQLGRTVPVSATTFSPWNLCATVATIAAVAAALA
                     LAAPRDGDPVREVEADLRDAGPRVDEEVVTPADRLDASRIVTSAVGLALAAWLALHFV
                     RGGSLTLDTVNWTFLALVCLLVRSPFELIALVKGAASTVGDILLQFPLYAGILGIMTT
                     TGLIAVLSDAFVGLASPRTFGFLSLLSAGLVNFFVPSGGGQFAVQGPILLDAAARIGV
                     APEVAIMALAYGDQWTNMVQPFWALPLLAIAGLKVRDILAYTTVTLVASGLVFAATLL
                     IVGAG"
     gene            complement(2512..3267)
                     /locus_tag="PLP50_17400"
     CDS             complement(2512..3267)
                     /locus_tag="PLP50_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA53372.1"
                     /translation="MDGRTNLVSGLLLLAVLAPLAGIALASPPELEKDVIPTSAGPLE
                     VTFVGHGSLFFRFGGKVLHVDPYGKLADYAALPKADVVLLTHAHGDHLDPAAIAAVRT
                     ATTKLVVAPACTGKVEGATVLPNGETTTVEGVRVEAVPAYNLVAKRPDGSPFHPKGEG
                     NGYVLTFGKTRVYVAGDTENVPEMKALEGIDVAFLPMNLPYTMTPEMVADAARAFRPR
                     VLYPYHFGETDPQKLVSLLGDEKGIDVRVRKMK"
     gene            complement(3404..>3886)
                     /locus_tag="PLP50_17405"
     CDS             complement(3404..>3886)
                     /locus_tag="PLP50_17405"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HPA53373.1"
                     /translation="SELGAAEAPHPWIFNSGIVTAGVAALLAGPGFAFALRRLGSDRL
                     PAVLTGLTISLWGVAMVLGGSFPMPDPRHGGFGLGLPLLVAPLFLLWGLRHTPDAGRL
                     RLFLLVVFLAMTALFAVMMGVGGLVTLANVGLWQRGNALASLPWIGIVAHALRRRIER
                     "
BASE COUNT          436 a         1431 c         1387 g          632 t
ORIGIN      
        1 cgcgtgatgg cgctgacgat cccgcagtgg atcgggaccc gctgggagtc gaaggggatg
       61 cggctcgcgt tcgtcgtcct ctcggccggc ctgctcgcgt tcgcgacgct gatcgtcgtc
      121 gccctcgcgc acgtcctcgc gggcctcctc ggcgtccccg cggtggcggt ggccgccgcg
      181 gtcctcctct acgtcctcct cgcgatggcg atggggggcg cgacggcgca cgcctggatc
      241 aacgcgttcc aggcgatcgt catgctcgtc gtcgcggtgc tcctcgtcgg gaccgcggtc
      301 ggccaggccg cgccgttcgg cggggtgctc gagcgcctcg gcgcgatcca gccgtcgctc
      361 gcgaccgtcg tgaaccccgc gtctccctac ttccggaacc tcttcgaggt cttcttctgc
      421 aacttcctcg tcggcctcgc catcgtcggg cagccgcaca tcctgtcgaa ggcgctctac
      481 ctgtccgatg gccggcaggt gaagcggtac ctcggcgtcg cgatcggggc cgggctcgtc
      541 ttcatggggg tcctctgggt cggcctcgcg gcgcgcctct ccctcccggc gctcccgcgg
      601 atcgacctcg tcgtcccgag ctggatcggc gcctccttcg ggccgggcct gagcgtcgtc
      661 gtcgccgtcg gaatcatctg cgccggtctc tcgacgctcg aggggatctt cctcgccctc
      721 gcgagcatcc tctcggtcga cgtccacccg ctcctgccgg gggcgaagcc cgagagggcg
      781 ctccgcttcg ggcggatcgg tctcgccgtg gtcgccgtcg tctgcctcct cctcgcccgc
      841 tggcagatcg cccacccgac cggagggtcg gtcgcgatct tcgcgcagta cggcatctac
      901 ctcctcttca ccgcctcctc cgtcccgctc gtcgccggga tgttcctgcc gcgcgcgcgg
      961 cgcggcgccg tcgcgctcgc ggccacggcg gccgtcgtcg tctacgtcgt cgccggggcg
     1021 ctgaagctcg gcgcgctctc gaacaacccg gcggtcctcg cgacgtgggg cctcctcgcc
     1081 tcctgggccg tcctcggggc ctccctcctc ctcgcgggga ggcgcgaggc atgagttctc
     1141 cggacgcgcc gcggtccggg gcggtcgcgt ccctcatgcg gccgctgaat acgctcgtcg
     1201 agcgcttcct gccgagcgcg ctggtcttcg ccgtcgtcct gacggggatc gtcgctgtcc
     1261 tcgccctcct cctgaccgac gctggccccg cagaggtcct gcgcggctgg ggcgacgggc
     1321 tctcggggct cctcgcgttc acgacgcaga tggcgctcat cctgatgctc gggcacgtcc
     1381 tcgcgagcac gcggcccgtg cgcgccctcc tcgcccggct cgggagcctt ccgcggaccc
     1441 cgcgccaggc ctacgtctcg gtcttcctcg tcgccgcgct ctcctcgctc gtgacgtggg
     1501 gcctcggcct cgtcgtcggg gcgctccttg cacgcgaggt cgccgcgcag ggacgggcgc
     1561 ggggcctccg gctccacttc ccgctcctcg tcgccgcggg cttctccggc ttcgtcgtct
     1621 ggcacatggg ctcctcgggc tccggaccgc tcaccgcggc gacggagggc tccttcatcg
     1681 cggctcagct cggccggacc gtgccggtct cggcgacgac gttctccccc tggaacctct
     1741 gcgccacggt cgcgacgatc gcggccgtcg ccgccgccct cgccctcgcg gcgccccgcg
     1801 acggcgaccc ggttcgcgag gtcgaagcgg acctgcgcga cgccgggccc cgggtcgacg
     1861 aggaggtcgt cacgccggcc gaccggctcg acgcgagccg catcgtcacc tcggcggtcg
     1921 ggctcgcgct cgcggcctgg ctcgcgcttc acttcgtgcg cggcggctcg ctgacgctcg
     1981 acaccgtgaa ctggacgttc ctcgccctcg tctgcctcct cgtgaggagc ccgttcgagc
     2041 tgatcgccct cgtgaaggga gcggcctcga cggtgggcga catcctcctg cagttcccgc
     2101 tctacgccgg gatcctcggg atcatgacga cgacggggct catcgccgtc ctctccgacg
     2161 ccttcgtcgg cctcgcgtcg ccgcgcacct tcggcttcct ctcccttctc tcggccggcc
     2221 tcgtgaactt cttcgtcccg tcgggcggag ggcagttcgc cgtccagggg ccgatcctcc
     2281 tcgacgcggc cgcgcggatc ggcgtcgcgc ccgaggtcgc gatcatggcg ctcgcctacg
     2341 gcgaccagtg gacgaacatg gtccagccgt tctgggcgct cccgctcctc gccatcgcgg
     2401 ggctgaaggt gcgcgacatc ctcgcctaca cgacggtcac gctcgtcgcc tcgggcctcg
     2461 tcttcgcggc aacgctcctg atcgtcgggg cgggctgacg ggccggccgg gctacttcat
     2521 cttccgaacg cggacgtcga tccccttctc gtcgccgagg agagagacga gcttctgcgg
     2581 gtcggtttcg ccgaagtggt acgggtagag gacgcggggc cggaacgcgc gggcggcgtc
     2641 ggcgaccatc tcgggcgtca tcgtgtaggg aaggttcatc gggaggaagg cgacgtcgat
     2701 cccctcgagc gccttcatct ccgggacgtt ctccgtgtcg ccggcgacgt agacgcgggt
     2761 cttcccgaac gtcaggacgt agccgttccc ctcgcccttc gggtggaagg gggagccgtc
     2821 gggccgcttc gcgacgaggt tgtaggcggg gaccgcctcg acgcggaccc cctcgaccgt
     2881 cgtcgtctcg ccgttcggaa ggaccgtcgc cccctcgacc tttcccgtgc aggcgggggc
     2941 gacgacgagc ttcgtcgtgg ccgtccggac cgcggcgatc gcggcggggt cgaggtggtc
     3001 gccgtgggcg tgcgtgagga ggacgacgtc cgccttgggg agcgcggcgt agtcggcgag
     3061 cttcccgtac gggtcgacgt gaaggacctt gcccccgaag cggaagaaga ggctgccgtg
     3121 ccccacgaac gtgacctcga gcgggccggc cgaggtcggg attacgtcct tctcgagctc
     3181 gggcggagac gcgagcgcga tgccggcgag cggcgcgagc acggcgagaa ggagcagccc
     3241 ggaaacgagg ttcgtccgtc cgtccatcgg agcctcctcg cggccgggtc gtcgccggcc
     3301 gcgaggagga tcgtgatacg gatcggtccc ggcgtcgagg gcgcggctgg gaggccggga
     3361 catcccctct cgaatcgggc gatcgccttc gcttcgaccg cggttagcgc tcgattcgcc
     3421 ggcgcagcgc gtgcgcgacg atcccgatcc aggggaggga ggccagagcg ttcccgcgct
     3481 gccagagacc gacgttcgcg agggtgacga ggccgccgac gcccatcatc acggcgaaga
     3541 gcgccgtcat cgcgaggaag acgacgagga ggaagagccg gaggcgtccg gcgtcgggcg
     3601 tgtgacgcag tccccagagc aggaagagtg gcgcgaccag gagagggagg ccgaggccga
     3661 atccgccgtg gcgcgggtcg ggcatcggga aggagccgcc gaggaccatc gcgacgcccc
     3721 agagggagat cgtgagcccc gtgaggacgg ccggcaggcg gtcgctcccc aggcggcgaa
     3781 gcgcgaaggc aaagccggga ccggcgagga gcgcggcgac tcccgcggtg acgatgccgg
     3841 agttgaagat ccaggggtgg ggcgcctccg ccgcgccgag ctcact
//
LOCUS       DAOONL010000236         3784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_29296_length_3784_cov_16.755162, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000236 DAOONL010000000
VERSION     DAOONL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29296_length_3784_cov_16.755162"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLP50_17410"
     CDS             <1..480
                     /locus_tag="PLP50_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HPA53374.1"
                     /translation="WYWWRINAWSEISAMGASGVVSLAAMAWFARPGAVTPGPGADFE
                     AQAKTMLVTVVLSTLVWVAVTLLTKPEPDEKLRAFYERVRPGGPGWARISASLGLGRE
                     PIPGGALSLANWALSVVLVYAALFGIGKLVLGATVAGLALLALAGACLALVMRNLRG"
     gene            complement(458..1147)
                     /locus_tag="PLP50_17415"
     CDS             complement(458..1147)
                     /locus_tag="PLP50_17415"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPA53375.1"
                     /translation="MDGAEKTYRIDRSLLLGELPLLAILLADLAFGLWALPRLPERVP
                     VHWNAAGEADAFGASWPHAVLPPLVGLTLWTVVLLLPLVDPLRRNYARFPGTLKLVRW
                     LLPLMCVAVHMTVTLSGLGLPVDDGATVRAIVAVFFVVFGNSMAKLRHNWFVGIRTPW
                     TIASEEVWTRTHRLAAPIWVTGGLVQLVGALLPGGVGEVLFPATLAAMVVVPIGYSLL
                     LFRTLNPGGSA"
     gene            complement(1134..1424)
                     /locus_tag="PLP50_17420"
     CDS             complement(1134..1424)
                     /locus_tag="PLP50_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPA53376.1"
                     /translation="MSDTGLVFKALSDPTRRRILEYLREGDLNAGEIAERFDMTKPSI
                     SHHLSLLKQAGLVEDTRRGQNIVYSLDTTVFQEALRFLLGMTEGKGKSHGRR"
     gene            complement(1421..2785)
                     /locus_tag="PLP50_17425"
     CDS             complement(1421..2785)
                     /locus_tag="PLP50_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA53377.1"
                     /translation="MPLSRPGLILVATLTFAGASASPADPPKAAAAEPAVRTDALARE
                     IVGHLRKGDTAAVAARFSPQMAAALPAERFREVWNGLPAQLGTLKTLGEPVVRVVDGA
                     ANAWVPATFEKALVSLHMVFDAEGRLIGLRVLPGPPPTEWTPAPYADPSKAREREVTV
                     GSGEWALPGTLALPAEGSSFPALVLVHGSGPSDRDATVGGAKVFRDLAGGLAARGVAV
                     LRYEKRTKVHGAKMVGRPVTFDEEVVDDAIFAAALLRATPGIDPKRVAVLGHSLGGTL
                     APRIATRDRALAGIVLLAGAVRAPEDLVEDQLDYLASIGAAPKDVVAATKADVAKLRA
                     LSPGSPEAATATLLGVPASYWLSFRGYDPAATAKGLGIPILVLHGGRDYQVTAKDLQR
                     WKTALEGDPRATIRLFPSLNHLFVAGEGPSSPVEYEMPGHVAPEVIEAIAAWAKALPA
                     RSVP"
     gene            2909..3295
                     /locus_tag="PLP50_17430"
     CDS             2909..3295
                     /locus_tag="PLP50_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53378.1"
                     /translation="MLKWVQCVRRRPDLTVVEFRRQWQRYGEEIVSLGAELGAVRVEV
                     SAALAVGANAEWQARRGSAPPFDGLAEIHFSGSAPEFFAALEAPGPKARVERLEALQE
                     ELVDVSRSCFFLASQDAEASFSVGEG"
     gene            complement(3302..>3784)
                     /locus_tag="PLP50_17435"
     CDS             complement(3302..>3784)
                     /locus_tag="PLP50_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53379.1"
                     /translation="LFVTFETPAGRKAPGYAGARTAAPADGGGAYGLFTPGTGLAECA
                     FREAWLFGLRQDASERSNLALVNVEPDGGPITLRWEAFDATAGRRIGASRDLVLGPGE
                     WRQVDGVLGDFLLRAGYLKVSRVSGNGRFLAYAVLNDGPAPGGPGTHDGSYVPMEAAE
                     "
BASE COUNT          515 a         1360 c         1393 g          516 t
ORIGIN      
        1 tggtactggt ggcggatcaa cgcctggagc gagatctcgg cgatgggcgc ctccggcgtc
       61 gtctcgctcg ccgccatggc gtggttcgcc cgccccggcg ccgtgacgcc cggcccaggc
      121 gccgacttcg aggcgcaggc caagacgatg ctcgtgaccg tcgtcctctc cacgctcgtc
      181 tgggtcgccg tgacgctcct gacgaagccg gagcccgacg agaagctccg cgccttctac
      241 gagcgcgtcc ggccgggcgg tcccggctgg gcgcggatct cggcgtcgct gggcctcggc
      301 cgcgagccga tccccggcgg agcgctctcg ctcgcgaact gggcgctctc cgtcgtcctc
      361 gtctacgccg cgctcttcgg catcggcaag ctcgtcctcg gggcgacggt cgcgggcctc
      421 gccctcctcg ccctcgcggg cgcctgcctg gccctcgtca tgcggaacct ccggggttga
      481 gcgtccggaa gaggaggagc gagtagccga tcgggaccac gaccatcgcg gcgagcgtcg
      541 cggggaacag gacctcgccg acgccgccgg ggaggagcgc cccgacgagc tggacgagcc
      601 cgccggtcac ccagatcggg gccgcaagcc ggtgggtccg ggtccagacc tcctcgctcg
      661 cgatcgtcca cggcgtccgg atgccgacga accagttgtg gcggagcttc gccatgctgt
      721 tgccgaagac gacgaagaag acggcgacga tcgcgcggac cgtcgcgccg tcgtcgaccg
      781 gcaacccgag ccccgagagc gtgacggtca tgtggacggc cacgcacatc agcgggagga
      841 gccaccggac gagcttcagt gttccgggaa agcgcgcgta gttccggcgg aggggatcga
      901 cgagcggaag aaggaggacg acggtccaga gggtgaggcc gacgagggga ggcaggacgg
      961 cgtggggcca cgacgcgccg aacgcgtccg cctcgccggc ggcgttccag tggaccggga
     1021 ccctctccgg caggcgcggc agcgcccaga ggccgaacgc gagatcggcg aggaggatcg
     1081 cgaggagcgg cagctccccg aggagaaggg agcggtcgat ccggtaggtc ttctcagcgc
     1141 cgtccatgag acttcccctt cccttccgtc attccgagga ggaaccgcag cgcctcctgg
     1201 aagaccgtcg tgtcgagcga gtagacgatg ttctgtccgc gccgcgtgtc ctcgacgagg
     1261 ccggcctgct tcagcagcga gaggtggtgg gagatcgacg gcttcgtcat gtcgaagcgc
     1321 tccgcgatct cgccggcgtt caggtccccc tcgcggagat actcgaggat tcgacgccgg
     1381 gtcgggtccg agagcgcctt gaagacgagg cccgtgtcgc tcacgggacg cttcgcgccg
     1441 ggagcgcctt cgcccacgcc gcgatcgcct cgatcacctc gggcgccacg tgcccgggca
     1501 tctcgtattc caccgggctc gagggcccct cgcccgccac gaagaggtgg ttcagggagg
     1561 ggaagagccg gatcgtcgcg cgcgggtcgc cctcgagcgc cgtcttccag cgctggaggt
     1621 ccttcgccgt cacctggtag tcgcgcccgc cgtggaggac gaggatcggg atgccgagcc
     1681 ccttcgccgt cgcggcgggg tcgtacccgc ggaaggagag ccagtaggag gccggcacgc
     1741 cgagaagcgt cgccgtcgcc gcttccgggg agcccgggga gagcgccctg agcttcgcga
     1801 cgtcggcctt cgtcgcggcg acgacatcct tcggtgccgc gccgatcgag gcgaggtagt
     1861 ccagctggtc ttcgacgagg tcctcgggcg cccgcacggc ccccgccagc aggacgattc
     1921 ccgcgagcgc gcggtcccgc gtggcgatcc gcggcgcgag cgtccctccg aggctgtgcc
     1981 cgaggacggc gacccgcttc gggtcgatgc ccggcgtcgc ccggaggagc gccgcggcga
     2041 agatggcgtc gtcgacgacc tcctcgtcga acgtgacggg cctcccgacc atcttcgccc
     2101 cgtggacctt cgtccgcttc tcgtaccgga ggacggcgac gccgcgggcc gcgaggcccc
     2161 cggcgaggtc gcggaagacc tttgcgccgc ccaccgtcgc gtcgcggtcg ctcggccccg
     2221 agccgtggac gaggacgagc gccgggaagg agctcccctc cgccggaagc gcgagcgtgc
     2281 cggggagcgc ccactcgccg cttccgaccg tgacctcccg ctcgcgcgcc ttcgacgggt
     2341 cggcgtacgg cgccggagtc cactccgtcg gcggcgggcc gggcaggacc cgaaggccga
     2401 tcagccgccc ttccgcgtcg aagaccatgt ggagcgagac gagcgccttc tcgaacgtcg
     2461 ccgggaccca ggcgttcgcg gccccgtcga cgacccggac caccggctcc ccgagcgtct
     2521 tcaacgtgcc gagctgcgcg gggaggccgt tccagacctc gcggaatcgc tccgccggca
     2581 gcgccgccgc catctgcggg gagaagcggg cggccaccgc ggccgtgtcc cccttccgca
     2641 ggtggccgac gatctcgcgc gcgagggcgt cggtccggac cgcgggctcg gctgcggcgg
     2701 ccttcggcgg atcggccggg gaggcggacg ccccggcgaa ggtgagtgtc gcgacgagga
     2761 tgagtccggg acgggaaagg ggcatggcgc ctccctgtat ttcgataatt agataaccat
     2821 ctaaatacta ggacggcttg ctctccgcgt caagggctat gatttggttt tccacggcgc
     2881 ccgcagcgcc cccgcgagga ggccgaccgt gctcaagtgg gtccagtgcg tccgacgccg
     2941 ccccgacctg accgtcgtgg aattccgccg ccagtggcag cgctacggcg aggagatcgt
     3001 ctccctcggc gccgagctcg gcgcggtgcg cgtcgaggtg agcgccgcgc tcgccgtcgg
     3061 ggcgaacgcc gagtggcagg ctcggcgggg ctcggcgccg ccgttcgacg gcctcgccga
     3121 gatccacttc tcggggagcg cccccgagtt cttcgcggcg ctcgaggcgc cggggccgaa
     3181 ggcgcgggtc gagcggctcg aggcgctgca ggaggagctc gtcgacgtct cccgctcctg
     3241 cttcttcctc gcctcgcagg atgcggaggc gtcgttctcg gtcggagagg gctgacctcc
     3301 cctactccgc cgcctccatc ggcacgtagc tcccgtcgtg cgtcccgggg cctcccgggg
     3361 ccggcccgtc gttgagcacc gcgtaggcga ggaaccggcc gttcccagag acgcgggaga
     3421 ccttcaggta gccggcccgg aggaggaaat cgccgaggac gccgtccacc tgccgccact
     3481 ctccgggccc caggacgaga tcgcgggagg cgccgatgcg ccggcccgcg gtcgcgtcga
     3541 aggcctccca gcggagcgtg atcggaccgc cgtcgggctc gacgttcacg agggcgaggt
     3601 tcgacctctc cgacgcgtcc tggcggaggc cgaagagcca cgcctcgcgg aacgcgcact
     3661 cggcgagccc cgtcccgggc gtgaagaggc cgtaggcgcc tcctccgtcg gccggcgcgg
     3721 cggtccgcgc tcccgcgtag cccggagcct tccgcccggc gggcgtctcg aacgtgacga
     3781 agag
//
LOCUS       DAOONL010000237         3771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_29444_length_3771_cov_12.979010, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000237 DAOONL010000000
VERSION     DAOONL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29444_length_3771_cov_12.979010"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLP50_17440"
     CDS             <1..315
                     /locus_tag="PLP50_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPA53380.1"
                     /translation="ELRKEGFHVAVDDLGSGYSNLSALAEIRPEFLKFDHLFTKDIHR
                     NRIKQDLLGAILSFAMKMETQVIAEGIESVDELQALRRLGVPLGQGFYLGRPGPIPAR
                     AA"
     gene            complement(346..1869)
                     /locus_tag="PLP50_17445"
     CDS             complement(346..1869)
                     /locus_tag="PLP50_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA53381.1"
                     /translation="MRRRLAFLAVAIPLSFWSCSGSEPVERPYVVSVPYDLDSLDPGH
                     RNRLSDFSILTSFYEPLVATDANLSARPALAQRWSNPDALTWTFELRPGVRFHDGSLL
                     GAEDVVWSFERLRAEPGLELAGHVSAIASVRARGERTVELRTTQPVAILLNKLRFVLI
                     VRRGESGAKLRERVNGTGPYRLAGLEPGREMRLERNEAYWGVHPDLREVRLLLNRAPA
                     EALEDLLSGRSDFVQSNAKATEEALRGRSGFVLRKQGSVSVKFLFVALNREDSRDVVG
                     GTNPFRDVRVRRALSLAIDRAELARRLSAPAIPMSQLVPPGIFGHDPALKALRHDPVE
                     ARRLLAEAGWPNGFSLELAARGLFAEAAGIVAEMISEVGIRARVRALPEAAWFRAMHG
                     GLLTLTISRYGCPTGDASDLFENALRTWDPAKRTGLNNYARYSSPEVDRLTDLAGRTL
                     EMSRRQGALREATARALEDLPVIPLYVDQDLYAFREGVAWTPRNDNFLIAAEIARPR"
     gene            1922..3547
                     /locus_tag="PLP50_17450"
     CDS             1922..3547
                     /locus_tag="PLP50_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA53382.1"
                     /translation="MSEESASASRPPARELERLRAEVEELRRLKAVDDLLETLTGVLD
                     IRQVFDRVSEISRAVLPHDAIALGRITPDRQSIRVWAHSAIPKGFDIPETVPLPDPTV
                     VQREWDFMVLDDISADETLRRQNAPLLKYGVKSVLRIPLKEDGMIVAALNFLSFRPDA
                     YAARDVLVAKRIAAFVHLALSHHDLAEAARRAAEAQERTRQLERRVEGLVAALAAHGR
                     TSRVIGVSASWKDVLRQAAKVASAETTVLLTGESGTGKEVVARFVHSSSPRASGPFVA
                     INCAALPDTLLESELFGFERGAFSGAHAAKPGRIEAAQGGVLFLDEVGETSPSVQAKL
                     LRVLQEREFQRLGGSRPQRADVRVVAATNRDLAAAMARGTFREDLFYRLAVFEIRLPP
                     LRERPDDILPLAGAFLEEIGAELGRPAAGISRDAMEALLGYRWPGNVRELRNVLERAS
                     ILSGGGLITHEHLIVPAPRSPASAPSSPERAGTVSPPRATRAPSLADLERDAIERALV
                     EARYNKSRAARALGLTRGQLYGRLRRLGMAPTD"
BASE COUNT          552 a         1399 c         1288 g          532 t
ORIGIN      
        1 gagctccgca aggaggggtt ccacgtcgcc gtcgacgacc tcggctccgg ctactcgaac
       61 ctctccgcgc tcgcggagat ccgccccgag ttcctgaagt tcgaccacct gttcacgaag
      121 gacattcaca ggaaccggat caagcaggac ctgctcggcg cgatcctctc gttcgcgatg
      181 aagatggaga cccaggtcat cgccgaaggg atcgagtcgg tcgacgagct ccaggcccta
      241 cgcaggctgg gcgtcccgct cgggcagggc ttctatctcg gccggcccgg cccgatcccg
      301 gcccgggccg cctgacgacc gccgcgccgc gcgaaaggag ccgtctcagc gcggccgggc
      361 gatctccgcg gcgatcagga agttgtcgtt gcgcggcgtc caggcgaccc cttcccggaa
      421 ggcgtagagg tcctggtcga cgtagagcgg gatgacgggc aggtcctcga gcgcccgcgc
      481 cgtcgcctcg cgcaacgccc cctgacgccg gctcatctcg agggtccgcc cggccaggtc
      541 cgtgagccgg tccacttccg ggctggagta gcgcgcgtag ttgttcaggc ccgtcctctt
      601 cgccgggtcc catgtccgga gcgcgttctc gaagaggtcg gaggcgtcgc ccgtcgggca
      661 gccgtagcgg ctgatcgtca gggtcaggag ccccccgtgc atcgcccgga accaggccgc
      721 ctccgggagc gctcgcacgc gcgcgcggat cccgacctcc gagatcatct ccgccacgat
      781 cccggccgcc tcggcgaaga gcccccgggc cgcgagctcg agcgagaagc cgttcggcca
      841 gccggcctcg gcgagcaggc ggcgggcctc gaccgggtcg tggcgaagcg ccttcagcgc
      901 cgggtcgtgg ccgaagatcc cgggcggaac gagctggctc atcgggatcg cgggcgccga
      961 gagtcggcgg gcgagctccg cgcggtcgat cgcgagggag agggcccggc ggacccggac
     1021 gtcccggaag gggttcgtcc cgccgacgac gtctctcgag tcctcgcggt tcagggcgac
     1081 gaagaggaac ttgacggaga cgctgccctg cttccgcagc acgaagccgc tgcggccgcg
     1141 cagcgcctct tccgtcgcct tcgcgttcga ctggacgaaa tcgctgcgcc ccgagaggag
     1201 gtcctcgaga gcctccgcgg gcgcgcggtt cagcaggaga cggacctcgc gaaggtcggg
     1261 gtggacgccc cagtaggcct cgtttcgctc gaggcgcatc tcccgccccg gctcgagccc
     1321 cgcgaggcgg taagggccgg tcccgttcac ccgctcgcga agcttcgctc cgctctctcc
     1381 cctccggacg atgaggacga aacgcagctt gttcaggagg atcgcgaccg gctgggtcgt
     1441 ccgcagctcg acggtcctct cccctctcgc ccggacgctc gcgatcgcgc tgacgtgccc
     1501 ggccagctcg agccccggct ccgctcggag ccgttcgaac gaccagacga cgtcctcggc
     1561 cccgaggagg ctgccgtcgt ggaaccggac tccgggacgc agctcgaagg tccacgtgag
     1621 cgcgtcggga ttgctccagc gctgggcgag cgccggccgc gccgagaggt tcgcgtcggt
     1681 cgccacgagc ggctcgtaga agctcgtcag gatcgagaag tcgctcagcc ggttccggtg
     1741 gccggggtcg agcgaatcga ggtcgtaggg aaccgagacg acgtacggcc gctcgacggg
     1801 ctcggacccg gagcacgacc agaaggaaag cggtatcgcg acggcgagga aggcgagccg
     1861 gcggcgcacg ttcgcgcgag tataggagcc cgctctccgc tctctccgta gaatccgcgc
     1921 gatgagcgag gagagcgcgt ccgcctcccg gccgccggcc cgcgagctcg agcgtctgcg
     1981 cgccgaggtc gaggagctgc ggaggctgaa ggccgtcgac gacctccttg agacgctcac
     2041 gggggtcctc gacatccgcc aggtcttcga ccgcgtctcg gagatctcgc gcgccgtcct
     2101 cccgcacgac gcgatcgcgc tcggacgcat cacgccggac cgccagtcga tccgcgtctg
     2161 ggcccattcg gcgatcccga agggcttcga catccccgag acggttcccc tccccgaccc
     2221 cacggtcgtc cagcgggagt gggacttcat ggtcctcgac gacatctccg ccgacgagac
     2281 gctccgccga cagaacgcgc cgctcctgaa gtacggggtc aagagcgtcc tccgtatccc
     2341 gctcaaagag gacgggatga tcgtggcagc gctcaacttc ctctcgtttc gacccgacgc
     2401 ctacgccgcg cgcgacgtcc tggtcgcgaa gaggatcgcc gccttcgtcc acctcgccct
     2461 ctcacaccac gacctcgcgg aggcggcgcg ccgcgccgcg gaggcgcagg agcgcacccg
     2521 tcagctcgag cgccgggtcg aagggctcgt cgccgccctc gcagcccacg gccggacctc
     2581 gcgcgtcatc ggcgtctccg cctcgtggaa ggacgtcctc cggcaggccg cgaaggtcgc
     2641 ctcggccgag acgaccgtcc tcctcacggg cgagtccggg accggcaagg aggtcgtcgc
     2701 gcgcttcgtc cactcctcct cgcctcgcgc ctcgggaccg ttcgtcgcga tcaactgcgc
     2761 cgcgctcccc gacacgctcc tggagtcaga gctcttcggc ttcgagaggg gcgcgttctc
     2821 cggggcgcac gcggccaagc cgggccggat cgaggccgcg caggggggcg tcctcttcct
     2881 cgacgaggtg ggcgagacga gcccttcggt ccaggcgaag ctcctgcgcg tcctccagga
     2941 gagggagttc cagcggctcg gcggaagccg gccccagaga gccgacgtcc gcgtcgtcgc
     3001 cgcgacgaac cgcgacctcg cggccgccat ggctcgcggc accttccgcg aggacctctt
     3061 ctaccgtctc gccgtcttcg agatccggct tcctcctctc cgggagcggc ccgacgacat
     3121 cctgcccctc gccggagcct tcctcgagga gatcggggcc gagctcggcc gtccggcggc
     3181 cgggatctcc cgggacgcga tggaggcgct cctcggttac cgatggcccg ggaacgtccg
     3241 cgagctccgg aacgtcctcg aacgcgcctc gatcctgagc ggcggcggcc tcatcacgca
     3301 cgagcacctc atcgtgccgg cgccgaggag cccggcgtcg gccccgtcct ctccggagcg
     3361 cgccgggacc gtctcgccac cccgcgcgac ccgagcgcct tctctggccg acctcgagcg
     3421 cgacgcgatc gagcgggctc tcgtcgaggc gcgctacaac aagagccgtg cggcgcgggc
     3481 tctcggcctg acgcggggcc agctctacgg ccggctccgt cgcctcggaa tggcgccgac
     3541 cgattgaccc gttcggagcg atatgctccg aacgagacac ttcgttcgaa cggcgtcacg
     3601 tcgactcgca ccgcgcacac gcgcataagc tcctccccga caacacgata gtgcgcggcg
     3661 acctccctcg aaggccttgg cagtcctctt gcggacgtcc gcgcgaagga tgtcccagat
     3721 gagccagaag accgtcgaac acatcatcgg ccggctcgcc acggacgagg a
//
LOCUS       DAOONL010000238         3768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_29476_length_3768_cov_17.880420, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000238 DAOONL010000000
VERSION     DAOONL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29476_length_3768_cov_17.880420"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLP50_17455"
     CDS             complement(<1..938)
                     /locus_tag="PLP50_17455"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPA53383.1"
                     /translation="MTEVLLLFLGMAVLLAPIVSLVVSLLGRSRLTKAEERLGALERS
                     RAALETELRALRAALGTRREGVAEAPPPAGEGAARPPVPAAAAPAPVPVRLEATDRIA
                     SPPPPFAAPPPVPAPVAAPVPAPPVPAAPPAPPAAPDVRPPAPPVIAVPPRPSAAPAG
                     AASRPAPPQAAGPAPGPTVPAAAPPRTAPPPPRPASAPAAPPKAPFDWESLVGVKLFS
                     WVAAVALVVAAVLFLRYSIDHGWLTPPLRMAIGLLTGIGLLVVCELKAARRYPVTANA
                     LDGAAIAVLFSTFFAAHALWKLVPALPTFGLMALVTG"
     gene            complement(963..1184)
                     /locus_tag="PLP50_17460"
     CDS             complement(963..1184)
                     /locus_tag="PLP50_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53384.1"
                     /translation="MPFLDEDEGVRADQCSRCERICEPAEMETCPVCGRRYCSYCRYR
                     IGSRSYCSRPCGDSFFFGGDVDDEGLSEE"
     gene            1240..1315
                     /locus_tag="PLP50_17465"
     tRNA            1240..1315
                     /locus_tag="PLP50_17465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1273..1275,aa:Met,seq:cat)
     gene            1354..2361
                     /gene="tilS"
                     /locus_tag="PLP50_17470"
     CDS             1354..2361
                     /gene="tilS"
                     /locus_tag="PLP50_17470"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPA53385.1"
                     /translation="MQNPAPPGRSEPLPDAFARHVAASGVLDGATAFVAACSGGSDST
                     ALALLLSALARRRDVPIVLFHVDHQLRGVEGHRDARAAADLAASLGTSFVFRSFPVRD
                     LREGGESLESAARRLRYESLVALGRDLGPGTLLATGHTLEDQAETVLLHLERRLGRTR
                     GGIRPRRTDGVVRPLLPFRRGELREWLLAEGVPWREDASNADERFARNRVRHRVLPEI
                     EARWPGATARLARAAEVLTERLDALDAAIDAALAEAGLPLGGPWPRRLLDRLGREAAG
                     RLLVRAAGGVGRPPGRAQVGRVLDRIAAGEPAFRESFAGRRITADRLTVRLTPPARLV
                     P"
     gene            2358..2921
                     /locus_tag="PLP50_17475"
     CDS             2358..2921
                     /locus_tag="PLP50_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPA53386.1"
                     /translation="MNLLYDERFSPAEIAHRVQSVGQRISSDFPESPIVLIAILKGAA
                     VFAADLARSLPRSVRLEYIDVIRGNEEEIVDFHFVTPFNVRGADIIILKDVIRSGIIE
                     TYLADQLREEGPTSIRFAAIVDRPQERKSSLLVDYVLFPSEDGVLVGYGMEFAGEGGN
                     FPFIAKVRTPGDPPFALATGPIKVVRG"
     gene            2994..>3768
                     /locus_tag="PLP50_17480"
     CDS             2994..>3768
                     /locus_tag="PLP50_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPA53387.1"
                     /translation="MSTTVKNVLLWLVILVVILLLYKAVDYGRTTPPLSFTEFMEMVT
                     KGEIDRVVIRGGEVRGYKKGVPEGREPSYRTYAPPYDDLVPDLQKANVKITAEEPRDG
                     GLFMILLQWAPILFLIGLWVFIMRQMQTGGNRAMAFGKSKAKLLTPNQKKVTFKDVAG
                     CDESKDELSEIIEFLKDPGKFQKLGGKIPKGVLMMGPPGTGKTLLARAVAGEANVPFF
                     SISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEID"
BASE COUNT          529 a         1369 c         1370 g          500 t
ORIGIN      
        1 cccgtcacga gcgccatcag gccgaacgtc ggaagggccg ggacgagctt ccagagcgcg
       61 tgggcggcga agaacgtcga gaagaggacg gcgatcgccg ccccgtcgag cgcgttggcc
      121 gtgaccgggt agcgtcgtgc cgccttcagc tcgcagacga cgagaaggcc gattcccgtc
      181 aggaggccga tcgccatccg gagcggcggg gtcagccagc cgtggtcgat cgagtagcgg
      241 aggaagagga ccgccgcgac gacgagcgcg accgcggcaa cccaggagaa gagcttgacg
      301 ccgacgaggc tctcccagtc gaacggggcc ttcgggggcg ccgcgggagc ggaagcgggc
      361 cggggaggcg gaggcgcggt ccgcggcggc gcggcagccg ggaccgtcgg cccgggggcc
      421 gggcccgccg cctggggcgg agccggccgc gaggccgctc cggccggcgc ggcggacggc
      481 cgggggggca cggcgatgac gggcggagca ggaggccgaa cgtccggcgc ggcgggcggc
      541 gcggggggcg ccgccgggac gggcggcgcg ggaaccggcg ccgcgaccgg cgccgggacg
      601 ggcgggggcg ccgcgaacgg ggggggcggc gacgcgatcc ggtcggtcgc ctcgagccgg
      661 accggaaccg gcgcgggagc cgcggcagcc ggcaccgggg gccgggccgc cccttcgccc
      721 gcggggggcg gcgcctccgc caccccttcg cgcctcgtcc cgagcgcggc gcgaagagcg
      781 cggagctccg tctcgagcgc ggccctcgac cgctcgagcg ccccaagacg ctcttcggcc
      841 ttcgtgagcc ggctccggcc gaggagcgag acgacgagcg agacgatcgg cgccaggagc
      901 accgccatcc ccaggaagag gaggagaacc tccgtcacgc gagcctcatc gccggcccgt
      961 cgctattcct cggagagccc ttcgtcgtcg acgtcgccgc cgaagaagaa cgagtcgccg
     1021 cagggacggc tgcagtagct gcgcgagccg atccggtacc ggcagtagct gcagtagcgc
     1081 cggccgcaga ccggacaggt ctccatctcg gccggctcgc agatccgctc gcaacgggag
     1141 cactggtcgg cgcgaacgcc ttcgtcttcg tcgaggaagg gcatgacgac atcatagacc
     1201 cgggatctgc taccatccgc ggctctgcgg ccggctcgtg gcgatgtagc tcagtcggta
     1261 gagcgggggt ctcataagcc ctgtgtcggc ggttcgagtc cgcccatcgc caccacccct
     1321 ccccgcccgg cccgcgtcgg ctaccctccc ggagtgcaga accctgcccc gccgggccgc
     1381 tccgagcccc ttccggacgc cttcgcccgc cacgtcgccg cttcgggggt cctggacggc
     1441 gcgaccgcgt tcgtcgccgc ctgctcgggc gggagcgact ccaccgccct cgctctcctc
     1501 ctctccgccc tggctcgccg tcgggacgtt ccgatcgtcc tcttccacgt cgaccaccag
     1561 ctccggggcg tcgaggggca ccgggacgcg cgcgccgcgg ccgacctcgc ggcgtcgctc
     1621 ggcacgtcgt tcgtcttccg gagctttccg gtccgcgacc tccgggaggg gggcgagagc
     1681 ctcgagtcgg cggcacggcg cctccgatac gagtccctgg tcgcgctcgg gcgggacctc
     1741 ggtccgggga cgctcctcgc gaccggccac acgctcgagg accaggcgga gacggtcctc
     1801 ctgcatctcg agcgccgcct cggccgcacg cgggggggca tccgaccccg ccggacggac
     1861 ggcgtcgtcc gccccctcct cccgttccgg cgcggcgagc tccgggagtg gctcctcgcg
     1921 gaaggggtcc cctggcgcga ggacgcctcg aacgccgacg agcgattcgc ccggaaccgc
     1981 gtccggcatc gggtccttcc ggagatcgag gcccggtggc cgggggccac ggctcggctc
     2041 gcgagggccg ccgaggtgct caccgagcgg ctggacgccc tcgacgccgc gatcgacgcc
     2101 gccctcgccg aggccgggct cccgctgggc ggcccgtggc cgcggcgcct cctcgatcgg
     2161 ctcgggcggg aggcggcggg ccgtctcctg gttcgcgcgg cgggaggcgt gggccgcccg
     2221 cccgggcgcg cgcaggtcgg ccgggtcctc gaccggatcg cggccggaga gcctgcgttc
     2281 cgggagtcgt tcgccgggcg ccggatcacc gccgaccggt tgaccgtccg cctgacgccc
     2341 cctgctagac tggtaccatg aacctcctct acgacgagcg gttctcgccc gccgagatcg
     2401 ctcaccgggt ccagtcggtc ggccagagga tctcgagcga cttcccggag agcccgatcg
     2461 tcctgatcgc gatcctcaag ggtgccgccg tcttcgcggc ggacctcgca cggtcgctcc
     2521 cgcggtccgt ccggctcgag tacatcgacg tgatccgggg gaacgaggag gagatcgtcg
     2581 atttccactt cgtgacgccg ttcaacgtcc gcggggccga catcatcatc ctgaaggacg
     2641 tgatccggag cggcatcatc gagacctacc tcgccgacca gctccgcgag gagggcccga
     2701 cctcgatccg cttcgccgcg atcgtcgacc gcccccagga gcggaagagc tcgctgctcg
     2761 tcgactacgt gctgttcccg tccgaggacg gcgtcctcgt cggctacggc atggagttcg
     2821 ccggcgaggg gggcaacttc ccgttcatcg cgaaggtgag gacgccgggc gaccctccct
     2881 ttgcgctcgc caccgggccg atcaaggtcg tccggggctg aactcgatgt cccgctgcgc
     2941 tgacgctcag cgggggcggc ccggcgccgt agattgccgg gaagggtgac cgtttgagca
     3001 cgaccgtcaa gaacgtcctc ctctggctgg tgatcctcgt cgtcatcctc ctcctctaca
     3061 aggcggtgga ctacggccgg accaccccgc ccctgagctt caccgagttc atggagatgg
     3121 tgacgaaggg ggagatcgac cgcgtcgtca tccgcggcgg cgaggtgcgc gggtacaaga
     3181 agggggtccc cgagggtcgc gagccgtcct accgcacgta cgccccgccg tacgacgacc
     3241 tcgtccccga cctgcagaag gcgaacgtca agatcacggc cgaggagccg cgcgacggcg
     3301 gcctcttcat gatcctcctg cagtgggccc cgatcctgtt cctgatcggc ctctgggtct
     3361 tcatcatgcg gcagatgcag acgggcggga accgcgcgat ggccttcggc aagtcgaagg
     3421 ccaagctcct gaccccgaac cagaagaagg tcacgttcaa ggacgtcgcc ggctgcgacg
     3481 agtcgaagga cgagctctcc gagatcatcg agttcctgaa ggacccgggg aagttccaga
     3541 agctcggcgg gaagatcccg aagggcgtcc tgatgatggg ccctcccggc acgggcaaga
     3601 cgctcctcgc ccgcgccgtc gccggcgagg cgaacgtccc cttcttctcg atctcgggct
     3661 ccgacttcgt cgagatgttc gtcggcgtcg gcgcgagccg cgtccgcgac ctcttcgagc
     3721 aggggaagaa gaacgccccc tgcatcatct tcatcgacga gatcgacg
//
LOCUS       DAOONL010000239         3718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_30023_length_3718_cov_7.867595, whole genome shotgun sequence.
ACCESSION   DAOONL010000239 DAOONL010000000
VERSION     DAOONL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30023_length_3718_cov_7.867595"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2305)
                     /locus_tag="PLP50_17485"
     CDS             complement(<1..2305)
                     /locus_tag="PLP50_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53388.1"
                     /translation="MRGGGPARRPRPIARLARAAAAALWLALPAAAQAPGVGAGEPAQ
                     LPPELRLLASEGTPSPANVPPYLDIDDLSMATWGGAVSIATETMRLVVGPMAPEEEKA
                     FDETWQPLFRFPHEKLVEWLNALNPLLGQYLGARAAAGEAVLGFDAAQLEAASAAAAG
                     DAATVAEAMESAALQERLLASLAARIAETVREIEALGPPPDPDEERSRRRKKHEAAFR
                     LLDGFPLEGEWVDEEGSVTQVKLLKRFPDGKVVFWSGPKTAFEKAAAAGIDTSKPGIS
                     YSEKTKGMVLMPGIYDLISAAEPLPDGRYVAVSWVLCPNAIAYSLEGDEIEIAGYQLQ
                     CLAGGGIGSSRARRTRRPLAGEPMVPPGKTWAEVVALGEKAAAGKVETLEQVKSQQPA
                     LWAAAVSGRLPEPTKGAAAAPTPRPTAPARSPEEEKVEKEQIAFHETNVLYFESQVAA
                     AERDVAAAKDPASREELTRRLLYAKDALQKERDAITTIRTGEYTRTRTEADALNLMIM
                     EKQGRDQAVYWSEVQRVLDHAPRLIAMAPDGERDHLKEFFHRQVTPEVIASGDLGRIR
                     RAATSIGTKVVGGLEKDAADAELEAVDASQRLAYVESVKKTCDTSLLVLSGFGGTGIA
                     TAYMGLTGYMQGGPTEAVKQAATTWSMAARVASDAMDAYTKGVVENYEAYAADPGKVR
                     LDERAAGLEGAAWSAGQAAAFAALMKGGSWALGRIVGGAGKGAAPPGPKPKWKDVVAE
                     ARFRQDQEGGRALVRAFRDKANQLASAG"
     gene            complement(2367..2948)
                     /locus_tag="PLP50_17490"
     CDS             complement(2367..2948)
                     /locus_tag="PLP50_17490"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPA53389.1"
                     /translation="MKKPLGLAALVLVVLALAAPPAAADALSLELGLHGGLVNSKDGE
                     GGSFLGGAQARVNLFWIVAAEARGSYYTDSYDLGELGSVDIENVPFQVSGMIYPIKLP
                     KIGVYLLGGGTYSNLKVTGTSVVQGEVTEKGWSTHVGAGIDLKVGEKTTLNGDVRAVF
                     LDASSVDELIDSARSNYKGDFWTVTIGLNYKLF"
     gene            complement(3058..3531)
                     /locus_tag="PLP50_17495"
     CDS             complement(3058..3531)
                     /locus_tag="PLP50_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPA53390.1"
                     /translation="MAGKVNPIPEGYGTVTPYLCVKGAAAAIELWKKALGAEELSRMP
                     GPGDMVMHAEVKIGSSIVMLSDEFPDFGQLGPTSRGGSTCTMMMYVPDCDAAWKRAVD
                     AGCTSTSEPKDEFWGDRFAKVTDPFGHQWAFATHVEDVSPEEMQKRAAALFEKGC"
BASE COUNT          455 a         1391 c         1282 g          590 t
ORIGIN      
        1 tcccggccga ggcgagctgg ttcgccttgt cgcggaaggc gcggacgagg gcgcgtcctc
       61 cttcctggtc ctgacggaag cgcgcctcgg cgacgacgtc cttccacttc ggcttcggac
      121 cggggggcgc cgcgcccttc cccgcgccgc cgacgatccg tccgagggcc cacgagcccc
      181 ccttcatgag cgccgcgaag gcggccgcct gcccggcgct ccaggccgcc ccctcgaggc
      241 cggcggcccg ctcgtcgagc ctcaccttcc ccgggtcggc ggcgtacgcc tcgtagttct
      301 cgacgactcc cttcgtgtag gcgtccatcg cgtcgctcgc gacgcgcgcg gccatcgacc
      361 acgtcgtcgc ggcctgcttg accgcctccg tcggcccgcc ctgcatgtag cccgtgagcc
      421 ccatgtaggc cgtcgcgatg ccggtcccgc cgaagcccga gaggacgagg agcgacgtgt
      481 cgcacgtctt cttcacgctc tcgacgtacg cgagccgctg cgaggcgtcg accgcctcga
      541 gctcggcgtc ggcggcgtcc ttctcgagcc cgccgacgac cttcgtcccg atcgacgtcg
      601 ccgcgcggcg gatcctcccg aggtctcccg acgcgatcac ctccggggtc acctgccggt
      661 ggaagaactc cttcagatgg tcccgctccc catccggggc catcgcgatg agccgcggcg
      721 cgtggtcgag gacccgctgg acttcgctcc agtacaccgc ctggtcccgg ccctgcttct
      781 ccatgatcat caggttcagg gcgtcggcct cggtccgggt ccgcgtgtac tcgccggtcc
      841 ggatcgtcgt gatcgcgtcc cgctccttct ggagggcgtc cttcgcgtag aggagccggc
      901 gggtcagctc ctcgcgcgag gcggggtcct tcgccgcggc gacgtcccgt tccgcggctg
      961 cgacctgcga ctcgaagtag aggacgttcg tctcgtggaa agcgatctgc tccttttcga
     1021 ccttctcctc ctccgggctc cgcgcgggcg cggtcggcct cggggtcggc gcggcggccg
     1081 cccccttcgt cggctccggg agccgccccg acacggcagc cgcccagagc gcgggctgct
     1141 gcgacttcac ctgctcgagc gtctcgacct tcccggccgc ggctttctcc ccgagcgcca
     1201 cgacctcggc ccaggtcttc ccggggggga ccatcggctc tcccgcgagc ggtcgacggg
     1261 tccgccgggc acggctcgag ccgatccccc ctccggcgag gcactggagc tggtagcccg
     1321 cgatctcgat ctcgtcgccc tccaggctgt aggcgatggc gttcgggcag aggacccacg
     1381 acaccgcgac gtaccgtccg tccgggagag gctcggccgc ggagatcagg tcgtagatcc
     1441 ccggcatcag gaccatcccc ttcgtcttct ccgaatagct gatcccgggc ttgctcgtgt
     1501 cgatgccggc ggccgccgcc ttctcgaagg cggtcttcgg ccccgaccag aagacgacct
     1561 tcccgtccgg gaagcgcttg aggagcttga cctgggtaac cgagccttct tcgtcgaccc
     1621 actccccctc gagcgggaag ccgtcgagga gccggaacgc cgcctcgtgc ttcttcctcc
     1681 ggcggctccg ctcttcgtcc gggtccggcg gcgggccgag cgcctcgatc tcccggaccg
     1741 tctccgcgat ccgcgcggcg agcgaggcga ggagacgttc ctggagggcg gcggactcca
     1801 tcgcttcggc gaccgtcgcg gcgtcgccgg ccgcggcggc cgaggcggcc tcgagctggg
     1861 ccgcgtcgaa gccgaggacg gcctcgccgg ccgcggcgcg cgcgccgagg tactggccca
     1921 ggagcgggtt cagggcgttc agccactcga cgagcttctc gtgcgggaag cggaagagcg
     1981 gctgccacgt ctcgtcgaac gccttctcct cctccggcgc cataggcccg acgacgagcc
     2041 gcatcgtctc cgtcgcgatg gagacggcac cgccccaggt cgccatggag aggtcgtcga
     2101 tatcgaggta cggcgggacg ttcgcgggcg agggcgtccc ctccgaagcg aggagccgga
     2161 gctcgggggg gagctgagcc ggctcgccgg cccccacgcc cggcgcctgc gccgcggcgg
     2221 ggagcgcgag ccacagggcg gcggccgcgg cgcgcgcgag ccgcgcgatc ggtcgggggc
     2281 ggcgggccgg cccacctccg cgcatgccgc ggacagcgta gcaccgcccg cgggccggag
     2341 acgcccccgg cccgcgggct ctcgggtcag aagagcttgt agttcagtcc gatcgtcacg
     2401 gtccagaagt cgcccttgta gttgctccgg gcgctgtcga tcagctcgtc gacgctgctg
     2461 gcgtcgagga agacggcgcg aacgtcgccg ttcagcgtcg tcttctcgcc gaccttgagg
     2521 tcgatgccgg cgccgacgtg ggtcgaccac cctttctcgg tcacctcgcc ctggacgacg
     2581 ctcgtccccg tcaccttcag gttgctgtag gtgcccccgc cgaggaggta gacgccgatc
     2641 ttcgggagct tgatcgggta gatcatcccg gagacctgaa acgggacgtt ctcgatgtcc
     2701 acggagccga gctccccgag gtcgtagctg tcggtgtagt acgagccgcg cgcctcggcc
     2761 gcgacgatcc agaagaggtt gacgcgcgcc tgcgcgccgc cgaggaagct cccgccctcg
     2821 ccgtccttgc tgttcacgag gccgccgtgg agccccagct ccaggctgag ggcgtccgcc
     2881 gcggcgggcg gagccgcgag ggccaggacc acgaggacca gggccgcgag gccgaggggc
     2941 ttcttcatct gcgggagtct ccttctctcg gaagtgaacg aagctaacac ggaaggagac
     3001 cgtccggcgc cgcgcgaggg gcggcggagc cggtcccccg gccccgccgc cccggcctca
     3061 gcagcccttc tcgaacaacg ccgccgcccg cttctgcatc tcctcgggag agacgtcctc
     3121 gacgtgcgtg gcgaaggccc actggtggcc gaacgggtcg gtcactttcg cgaagcggtc
     3181 gccccagaac tcgtccttcg gctccgacgt cgacgtgcag cccgcgtcga cggcccgctt
     3241 ccaggccgcg tcacagtccg ggacgtacat catcatcgtg caggtcgagc ctcccctcga
     3301 ggtcgggccg agctgcccga agtcggggaa ctcgtcggag agcatgacga tggagctccc
     3361 gatcttcacc tcggcgtgca tcaccatgtc gccggggccg ggcattcggc tgagctcctc
     3421 ggccccgagc gccttcttcc agagctcgat cgccgccgcg gcgcccttga cgcagaggta
     3481 gggggtgacg gttccgtacc cctcggggat gggattcacc tttccggcca tggtcgtctc
     3541 ctttcgtgtc cgccccgaag gggcatctct cccggccgcg gcgggttccc gcggacggtg
     3601 ctcacccggt gtgtcgttcc gatgttctac gcggggcggc cttccgggtt tccggccggc
     3661 gcccctttcc cgtaccgggc cgtgacgtag cccgtgacga ggccgacgag ggcgccgg
//
LOCUS       DAOONL010000240         3612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_31182_length_3612_cov_11.310093, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000240 DAOONL010000000
VERSION     DAOONL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31182_length_3612_cov_11.310093"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..342)
                     /locus_tag="PLP50_17500"
     CDS             complement(100..342)
                     /locus_tag="PLP50_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53391.1"
                     /translation="MDEDKRDESEAGNELENGVLSKRHWKGPVLAGGAGCALSRGDAD
                     PAARRGTGLGGARGFWLRFARGLLRAITPLRRASGA"
     gene            397..1239
                     /locus_tag="PLP50_17505"
     CDS             397..1239
                     /locus_tag="PLP50_17505"
                     /inference="COORDINATES: protein motif:HMM:NF025186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HPA53392.1"
                     /translation="MVDRGWLQTWSEPTERGGNPTWLYPTAAGRTAGLGLVLGAVEGT
                     SFQQLVATMLPSARTRPLELAPRIAPAFLRHQRETTTLLVRLAAAHGALWYSAWDRPL
                     PQASAPAQLPQPDGVLVLPSASGPELVFLEHDRGMESPAHFARAKAARYAELAVRPEL
                     CQRLLGFPTFRLAVTVENARLHRHPLERLGLLSRLLREAGAAREAVLSLAGWANAWPA
                     GAVWCRAEDPFLPASRPAVELQEPTLCHTLPCPPSGRGEPCACSAQGSPSRLLEGDGR
                     RPCA"
     gene            1518..2153
                     /locus_tag="PLP50_17510"
     CDS             1518..2153
                     /locus_tag="PLP50_17510"
                     /inference="COORDINATES: protein motif:HMM:NF036314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53393.1"
                     /translation="MCLLTRTDAMPGSSFSLPAKRTCPGVFLTPTSVCSACYADERKR
                     YRWRTVKLAQERRLAWAVHALTSGRFAPVVTELISRRDERHFRLHDSGDFFSPPYVDA
                     WYDIALALPEVSFWAPTRSWALHGHVRPDDDPLLVSLRRLAFLPNVTVRPSALLIDSD
                     PPVIAGLHAGSSVTTDPARPTCPKYLRSPPACGDCRHCWAEPMKPVVYLKH"
     gene            2243..3070
                     /locus_tag="PLP50_17515"
     CDS             2243..3070
                     /locus_tag="PLP50_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HPA53394.1"
                     /translation="MSPPVSIRWAELLADAVSRPGQILEAYSLFHGYSLGNQLAAMFQ
                     CRERRLAPGPIATLQRWNELKRRVRAGEKAIVLCMPVSVRVKDDERSRGPVPVGDAPR
                     LKTIFVWKPRWFVLAQTDGEPLTNAVEIPSWDEARALEALAVQRIPFDLPDGNVQGFA
                     HGRSVAVSPIAALPAKTLFHELGHVLLNHTAERGDDRPDLPKTLTEAEAEAVALLCLE
                     ALHLPGAEYARGYIQSWYGAGNPLPEHSAQRIFKAADTILRAGRSEPAPVAAGRGAA"
     gene            3067..3351
                     /locus_tag="PLP50_17520"
     CDS             3067..3351
                     /locus_tag="PLP50_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53395.1"
                     /translation="MRLDLSSVPLSPAGAHLGVRPDAWFRAPIDIDGVQHFLDLVSVR
                     VGRHGVQHAVSKDLDAMVRLHHLACGAYGPFATVTYAERPFVLFVTPSCQ"
     gene            3363..3602
                     /locus_tag="PLP50_17525"
     CDS             3363..3602
                     /locus_tag="PLP50_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53396.1"
                     /translation="MSEATSTHCEDCGATGGIRTYDDPETEQEVLLCPACARDLYSGV
                     EDVTEGLVSADDDPDSEFADACITAADYMRADEGY"
BASE COUNT          507 a         1428 c         1102 g          575 t
ORIGIN      
        1 ggcaattcac gcagtactct tctcttgccc acctgtcaag actatggccc cgtcactatg
       61 gccccgtcac tgagctcgcc cagcagcgcg gaggagacgt cacgcgccgg acgcgcgccg
      121 gagcggcgtg atcgcgcgca ggagaccgcg agcgaaccgc agccagaagc cacgcgcgcc
      181 gccgagaccg gtgcccctac gcgcagcagg gtccgcatca cccctcgaga gagcgcatcc
      241 ggcgccgccg gccaggaccg gccccttcca gtgtctcttc gagagcacgc cgttctcgag
      301 ctcgttaccc gcctccgact cgtccctctt gtcctcgtcc acgacgccct gttcaaagac
      361 gtctcgcggc aggtcgtcgg gcggttcgtc aatcggatgg tcgatcgcgg gtggctccag
      421 acctggtcgg agcccacgga gaggggaggg aaccccacgt ggctctatcc gaccgcagcc
      481 gggcgaacag cgggtctcgg tctggtcctc ggcgccgtgg agggaacttc ctttcagcag
      541 ctcgtcgcaa cgatgctgcc gtccgcgcgg acgcgccccc tcgagctcgc tccgcggatt
      601 gcaccagcct tcctccgtca ccagcgggag accaccaccc tcctcgtccg cctcgccgcg
      661 gcgcacggtg ccctctggta ctccgcctgg gatcggccgc tcccccaggc gagcgcgccg
      721 gcccagctgc cccaacccga cggagtcctc gtcctcccga gcgcgtccgg ccccgagctc
      781 gtcttcctcg aacacgaccg gggtatggag tcgcccgcgc acttcgcccg ggcaaaggct
      841 gcgcgctacg ccgagctggc cgtccggccg gagctctgcc agcgccttct cggcttcccg
      901 acgtttcgcc tcgccgtgac agtcgagaac gcccgtcttc accgtcatcc tctcgagcga
      961 ctcgggcttc tctcccgact cctccgcgag gccggcgcgg cccgggaggc ggtcctctcc
     1021 ctggccggat gggcgaacgc atggccggca ggcgccgttt ggtgccgagc ggaagacccc
     1081 ttcctgccgg catcccggcc cgctgtcgag ctccaggagc cgactctgtg ccacaccctg
     1141 ccgtgtccac cgtccgggcg aggggagccg tgtgcgtgtt ccgcacaggg ctccccgtcg
     1201 cgcctcctcg agggcgacgg ccgccggccg tgcgcctgac gcacgtcccc gggatcggct
     1261 ctcacgtttt cgcttccggt gtggtctctt tctggcgtcg gcacggccgc agctccttcc
     1321 caactcgctt cggcacccgc cggtctcccg gtttccggga cctcctcggc cggcgggtgc
     1381 tccccggccc tctgagctct ctggcggctg gcgtctctgc acccgaatag gtccgctcct
     1441 ttgcttggga ccttcggtcg agacgtcggc cgcccgaggg cgccgaggcg cccgggaatt
     1501 catcgaggag gtcctaagtg tgcctgctca cgcgcacgga cgcgatgccg gggagctcct
     1561 tcagcctccc ggccaagcgc acctgccccg gggtctttct cacgcctacg tcggtttgta
     1621 gcgcctgcta cgccgatgaa cggaagcgct acaggtggcg aaccgtaaag ctcgcccagg
     1681 aaaggcggct cgcctgggct gttcacgctc tcacctccgg acgcttcgcc ccggtcgtca
     1741 cggagctcat ctctcgtcga gacgaacgcc acttccgcct ccacgattcc ggcgacttct
     1801 tcagcccacc ctacgtcgac gcctggtacg acatcgccct cgcgcttccc gaggtctcgt
     1861 tctgggcgcc gacgagaagc tgggccctcc acggccacgt ccgccccgac gacgatccgc
     1921 tcctcgtctc ccttcgccgt ctcgcttttc tgccgaacgt caccgtccgc ccgagcgcac
     1981 tcctgatcga cagcgacccg cccgtcatcg ccggtctgca cgcggggagc tccgtcacga
     2041 cggatcccgc gcgccccacc tgtccgaagt acctcaggag cccaccggct tgtggcgact
     2101 gccgccactg ctgggccgag cccatgaaac cggtggtcta cctcaagcac tgaactgacc
     2161 cgccacctcg acgtcgtgcc ccggacgaaa caccgtcccg ggcgcgaggg cgtccgggac
     2221 ctgacacgca aggaggtccc tgatgtctcc acccgtctcg attcgctggg cggagctcct
     2281 cgctgacgcg gtctcccgcc ccggccagat cctcgaggcg tacagcctct tccacggcta
     2341 cagcctcgga aaccagctcg cggccatgtt ccagtgccgc gaacgacgcc tcgcccccgg
     2401 gccgatcgcc accctccagc ggtggaacga gctcaagcgg cgcgttcggg ccggcgagaa
     2461 ggccatcgtc ctgtgcatgc cggtgagcgt ccgggtgaag gacgacgagc ggagccgcgg
     2521 gcccgtgccc gttggcgacg ctccgcggtt gaagacgatc ttcgtctgga agccgaggtg
     2581 gttcgtcctg gcccagaccg acggagagcc actcacgaac gccgtcgaga tcccgtcgtg
     2641 ggacgaggcg cgcgccctcg aggccctcgc ggtccagcgc atcccattcg acctgcccga
     2701 cggcaacgtc cagggcttcg cccacgggcg ctccgtcgcg gtaagcccca tcgccgcgct
     2761 cccggccaag accctcttcc acgaactcgg ccacgtcctc ctcaaccaca ccgccgagcg
     2821 cggcgacgac cggccggacc tcccgaagac gctcacggaa gcggaggccg aggcggtcgc
     2881 cctcctctgt ctcgaggcgc tccacctccc tggcgccgag tacgcccgcg gctacatcca
     2941 gtcgtggtac ggcgcgggca accccctccc ggagcactcc gcccagcgga tcttcaaggc
     3001 cgccgacacg atcctccgtg ccggtcgcag cgaacccgca cccgtcgccg cggggcgagg
     3061 ggccgcatga gactcgacct ctcctctgtc ccgctctcgc cggccggcgc ccacctgggc
     3121 gtcagacccg atgcctggtt ccgtgcgccg atcgacatcg acggcgtcca gcacttcctc
     3181 gacctcgtct cggtccgagt cggccgccac ggtgtccagc acgccgtctc gaaggacctc
     3241 gacgccatgg tccggcttca ccacctcgcc tgcggggcct acggcccgtt cgccacggtc
     3301 acgtacgccg aacgcccctt cgtcctcttc gtcaccccct cgtgccagta ggggagagcg
     3361 ctatgagtga agcgacctca acccactgcg aggactgcgg cgccacgggc ggcatccgaa
     3421 cctacgacga ccccgagacc gagcaggagg tgcttctctg tcctgcatgt gcccgcgacc
     3481 tctactccgg agtcgaggac gtcaccgaag gcctcgtctc cgcggacgac gatcccgaca
     3541 gcgagttcgc cgatgcctgc atcaccgctg ccgactacat gcgcgccgac gagggttact
     3601 gatggcgacc cc
//
LOCUS       DAOONL010000241         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_31898_length_3549_cov_16.850887, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000241 DAOONL010000000
VERSION     DAOONL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31898_length_3549_cov_16.850887"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PLP50_17530"
     CDS             <1..1170
                     /locus_tag="PLP50_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53397.1"
                     /translation="ENFVQAVPEDPDECRRMVLAKDVFALGMTLVRVVNGRFPDGVYT
                     SMTSLVQKKERLEEMPVSLPSSLPAELRELVLAMCRTEWRMRPPLPVVVRTFDALRRG
                     ATEEERARLVAAPRVEALPEAPPSEPVELVGPYRLVNRAFAERTPGDGQTRPLAELHD
                     PFGRRLVGVPYEFRSAAEESAFYEERKALLEDLNAVRLRHPELFPGSFRDLVRQGVGE
                     DLFVVWVVRPLLEGAIDLDRYLAEERPDASLGERIAILRRVAEALSALESGGYTLAAL
                     TPRLVFFVPPPASVGDAAPTVAGRTRPVAALFDIGAPASGRRFRQELMGTASAVRTSP
                     EATGRVVAGFFDVASRMRVLTGLDRDGRDAFLQLGDAQSWGERADLLRWLETRLG"
     assembly_gap    1519..1618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1619..2310
                     /locus_tag="PLP50_17535"
     CDS             <1619..2310
                     /locus_tag="PLP50_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53398.1"
                     /translation="RRAALTTSDGTAPREGGTVNMKATRILVVAGACALVFSTACGKK
                     ETPESEGKELSKNPIAAMGQIQEAVEKAAQAAKESQEMKAVDPVHFSKLIELLPKAPA
                     GFTADGDPRGETTNAMGFKVSTAEQSYSAEGKSLQVKLTDGAFNAPLYTFVTMASQFA
                     RESTEGYEKGVTYDGNPGVEKWRKDGGDAELNVVVGKRFFVEVQGSGITPELVREVWS
                     SIDRATLAGLK"
     gene            2318..3415
                     /locus_tag="PLP50_17540"
     CDS             2318..3415
                     /locus_tag="PLP50_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPA53399.1"
                     /translation="MTNIGIPKECRPGEFRVGISPVGVRMLTGAGHTCFVQSGAGDGS
                     GFLDEQYREAGATVVHSAEEAWRRADLVLKVSRPTVQELEWLQPGKTLMGYLYLAAAH
                     PGKVELLLEKKVSAVAYELIQLADGSLPVLKPLSQIGGRMAAQVAARLLQNDAGGHGR
                     LIGGVAGVPPTEVVVVGAGVAGEAAARAFLGLGAHVTVLDTDLARLQSIDERLSGKLV
                     TVVAHPFHVARAAEYADVLVGAVMVPGERAPVVFTRAMLRKMKPGSLFLDLSIDQGGC
                     AETSRPTTHESPTYVEDGVVHCCIPNLGGVVARTSTHAFLNAAAPWIERVVVQGIEAA
                     AAADPALAHGLVTHAGELRNFRRVSYARPVE"
BASE COUNT          492 a         1183 c         1291 g          483 t
ORIGIN      
        1 gagaacttcg tccaggccgt ccccgaggac ccggacgagt gccggcggat ggtcctcgcc
       61 aaggacgtct tcgcgctcgg gatgacgctg gtgcgggtcg tgaacggccg gttcccggac
      121 ggcgtctaca cgagcatgac gtcgctcgtc cagaagaagg agcggctgga ggagatgccg
      181 gtttccctgc cgtcctcgct tccggcggag ctccgcgagc tcgtcctcgc gatgtgccgg
      241 accgagtggc ggatgcgccc cccgctcccc gtcgtcgtcc ggacgttcga cgccctccgc
      301 cgcggcgcga ccgaggagga gcgggcccgc ctcgtcgccg cgccgcgcgt ggaggcgctg
      361 cccgaggcgc cgccgtccga gccggtcgag ctcgtcgggc cgtaccggct cgtgaaccgc
      421 gcgttcgccg agcggactcc gggcgacggg cagacgcggc cgctcgccga gctgcacgac
      481 ccgttcgggc ggcgcctcgt cggcgtcccc tacgaattcc gttcggcggc ggaggagagc
      541 gcgttctacg aggagcggaa ggcgctcctc gaggacttga acgccgtccg cctccggcac
      601 ccggagctct tcccggggag cttccgcgac ctcgtgaggc agggggtggg ggaggacctg
      661 ttcgtcgtct gggtcgtgcg gccgctcctc gagggggcga tcgacctcga ccgctacctc
      721 gcggaggagc gcccggacgc ttcgctggga gagcggatcg cgattctcag gagggtcgcg
      781 gaggcgctgt cggccctcga gtcgggcggc tacacgctcg cggcgctgac gccccggctc
      841 gtcttcttcg tgccgcctcc cgcctcggtc ggcgacgcgg cgccgacggt cgccggccgg
      901 acgcgcccgg tcgccgcgct cttcgacatc ggggcgccgg cgtcgggaag acgattccgt
      961 caggagctga tggggaccgc ctcggcggtc cgcacgagcc cggaggcgac cggccgcgtc
     1021 gtggccggct tcttcgacgt ggcttcccgg atgcgggtcc tgaccgggct cgatcgggac
     1081 gggcgggacg ccttcctgca gctcggcgac gcgcagagct ggggcgagcg ggccgacctt
     1141 ctccgctggc tcgagacccg gctcggctag cggttccctc cggcgggcct cgaggtttcg
     1201 ggtctcttcg gcgcggctcg aggcgacctg cgggcgctgc cggaaagaga gacggcgggg
     1261 tctgcccccg ccgtcgaaga tcgttgcccc ggcgtcccgg tcctatcgca ggagagtaga
     1321 ggaaggattg cgaaaggtgt ggaccaggat ggaaggcgga aaggtgcgga agtggtggag
     1381 gttggacggg atgggactcc tccgccaggt ccgggagccg gggtagcgag ccgtctgagc
     1441 cgtcatgcgg gcctcccggc cgctctcgga gcggggaggg gggcccggag gctcgccttc
     1501 tggagcctcg ggtcgaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1621 cggcgggcgg ccctgacgac gtcggacggg accgcccctc gcgaaggagg aaccgtgaac
     1681 atgaaggcga cccgaatcct cgtcgtcgca ggggcctgcg ccctcgtctt ctccaccgcc
     1741 tgcggcaaga aggagacgcc cgagagcgag gggaaggagc tctcgaagaa cccgatcgcg
     1801 gcgatgggcc agatccagga agccgtcgag aaggcggcgc aggcggcgaa ggaatcccag
     1861 gagatgaagg cggtggaccc ggtccacttc tcgaagctga tcgagctcct cccgaaggcg
     1921 ccggccggct tcacggcgga cggggacccc cgcggcgaga cgacgaacgc gatgggcttc
     1981 aaggtctcga cggccgagca gagctactcc gcggagggga agagcctcca ggtgaagctg
     2041 acggacgggg ccttcaacgc tccgctctac acgttcgtga cgatggcctc ccagttcgcg
     2101 cgcgagtcga ccgaggggta cgagaagggc gtcacgtacg acgggaaccc cggagtcgag
     2161 aagtggcgga aggacggcgg agacgccgag ctgaacgtcg tcgtcgggaa gcgcttcttc
     2221 gtcgaggtcc agggctccgg gatcaccccc gagctcgtcc gcgaggtctg gtcgtcgatc
     2281 gaccgggcga cgctcgccgg cctgaagtga ggggcgcgtg acgaacatcg ggattccgaa
     2341 ggagtgccgt cccggagagt tccgggtcgg gatctcgccg gtcggcgttc ggatgctgac
     2401 cggggccggg cacacctgct tcgtccagag cggcgcgggc gacggctcgg gcttcctgga
     2461 cgaacagtac cgggaagccg gcgcgacggt cgtccactcg gccgaagagg cctggcgtcg
     2521 cgcggatctc gtcctgaagg tgagccgccc gactgtccag gagctcgagt ggctccagcc
     2581 cggcaagacg ctcatgggct acctctacct cgcggcggcg catcccggaa aggtcgagct
     2641 cctcctcgag aagaaggtct cggccgtcgc gtacgagctg atccagctcg ccgacggatc
     2701 gctcccggtc ctcaagccgc tcagccagat cgggggccgg atggcggcac aggtcgccgc
     2761 ccggcttctc cagaacgacg cgggcgggca cgggcggctc atcggcggtg tggccggcgt
     2821 cccgccgacg gaggtcgtcg tcgtcggcgc cggcgtggcg ggggaggccg cggcgcgggc
     2881 gttcctcggc ctcggagccc acgtcacggt cctcgacacc gacctcgcgc gcctgcagtc
     2941 gatcgacgag cgcctctccg ggaagctcgt gacggtcgtc gcgcacccat tccacgtcgc
     3001 ccgcgccgcc gagtacgccg atgtcctcgt cggggcggtg atggtcccgg gcgagcgggc
     3061 cccggtcgtc ttcacgcgcg ccatgctccg aaagatgaag ccgggctcgc tcttcctcga
     3121 cctctcgatc gaccaggggg gctgcgcgga gacgtcgcgg ccgacgacgc acgagtcgcc
     3181 gacctacgtc gaggacgggg tcgtccactg ctgcatcccg aacctcggcg gcgtcgtcgc
     3241 gcgcacctcg acgcacgcct tcctcaacgc ggccgctccg tggatcgagc gggtcgtcgt
     3301 ccagggcatc gaggccgccg ccgcggcgga cccggccctc gcgcacggcc tcgtcacgca
     3361 cgcgggcgag ctccggaact tccggcgggt ctcctacgcc cggcccgtcg aatgagacag
     3421 gaagcgaacg aaccacgatg agctggctcc acacctacca cgaccggatc gtcagcgccg
     3481 aggaggccgt ccgaacggtc aagtcgggcg accgtgtcta cctgaccggg aactgctccg
     3541 tcccgcagg
//
LOCUS       DAOONL010000242         3432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_33339_length_3432_cov_13.809002, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000242 DAOONL010000000
VERSION     DAOONL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33339_length_3432_cov_13.809002"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLP50_17545"
     CDS             <1..806
                     /locus_tag="PLP50_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPA53400.1"
                     /translation="GSFRYSDPAWHLPWIPVEGPGLFAIGVAAKVLQLYEGTPVAEGL
                     REAFERLSDVMPPEIRVSPGAFVERLYIHPQPIRLVDPAVWNAVATAIREKTVLAVRY
                     QKPSGETSARRLAPYGLVLAAGDWLVAAQDRDDDPGVVKTFYVSRIRGAETTPEPFSV
                     PRDFDLKRHFGETIGIFVGKEPFRFRVRFAKGMAAWVAEVRWHPRQKLTPLPSGEIEL
                     ELPAGSLFEARRFVLSFGKDAVALSPPELVAALRSETEEMAAAYRGAKG"
     gene            927..2474
                     /locus_tag="PLP50_17550"
     CDS             927..2474
                     /locus_tag="PLP50_17550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA53401.1"
                     /translation="MPDRELRPAAPPAWGLLLAIAGARLALHAALADRYGYFRDELYF
                     LDCGRHLAWGYVDAAPAIGLYAKLALLLGGSLPALRLLAGLAGAGVVLLTGLLAWRLG
                     GSRAAQGLAAVAALATPIFLAIASLFSMNVVEPLFWTGCAIVLVRLANGASPRLWLLL
                     GLLAGLGLLNKHSTAFFGVAVAAGILLSPARRALATPWPWAGAGVALLVFLPNLLWQA
                     TNGFPTLEDLRNVARTGKNVVLGPGEFVLTQVLQQGPHLLPLWLGGLAWLLLARSGRY
                     RALGAAFAVFFVLMFAMNAKGYYLTPIFPIVIAAGAVGLDEALARSRRLAGRAWPRLA
                     ATAWCVLLTLPAAPMVLPLLDPPVFVDYTRRLGFTPPRAEVAHEGPLPQMFGDQHGWP
                     ELVTEVARVWNGLTPEERARGAIFANNYGEAGAINLLGPAQGLPRAISAHQNHFFWGP
                     RGFRGDVLVVLQDDRVSLERLCSSVEEAGRHAHPWGMAEENGPIWVCRGLKTPLQELW
                     PRLKHWN"
     gene            2561..>3432
                     /gene="galE"
                     /locus_tag="PLP50_17555"
     CDS             2561..>3432
                     /gene="galE"
                     /locus_tag="PLP50_17555"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPA53402.1"
                     /translation="MSSILVTGGAGFIGSHTTLELLRTGHDVVVLDSLVNSRREAVDR
                     VGALAGREVPFVEADVRDRAALDAHFARHPVRAVIHFAGLKAVGESVEKPLAYWDVNV
                     CGSVALLDAMKQAGVKTLVFSSSATVYGDPASVPIPEDAPVGTPSNPYGRTKLVVEQL
                     LADVFASDPEWRIARLRYFNPVGADASGRIGEDPRGLPNNLMPYVAQVAVGRREALSV
                     FGDDYPTPDGTGVRDYIHVTDLAVGHLAALAKLEEGPGLRTWNLGTGQGQSVLEVVRA
                     FEEASGRKVPYRVVP"
BASE COUNT          412 a         1348 c         1173 g          499 t
ORIGIN      
        1 gcggctcgtt ccgctactcc gaccccgcct ggcacctccc ctggatcccc gtcgaagggc
       61 ccggcctctt cgcgatcggc gtcgccgcga aggtgctcca gctctacgag gggacgcccg
      121 tcgccgaggg gctccgcgag gcgttcgagc gcctctccga cgtgatgccg ccggagatcc
      181 gcgtcagccc cggcgcgttc gtcgagcggc tctacatcca cccgcagccg atccgcctcg
      241 tcgacccggc cgtctggaac gccgtggcga cggcgatccg cgagaagacg gtcctcgccg
      301 tccgctacca gaagccctcc ggcgagacga gcgcgcggcg gctcgcgccg tacggcctcg
      361 tcctcgcggc gggcgactgg ctcgtcgccg cgcaggaccg cgacgacgac cccggagtcg
      421 tgaagacgtt ctacgtctcg cgcatccgcg gcgccgagac gacccccgag ccgttctccg
      481 tcccccgcga cttcgacctg aagcgccact tcggcgagac gatcgggatc ttcgtcggga
      541 aggagccgtt ccgcttccgg gtccgcttcg cgaaggggat ggcggcgtgg gtcgccgagg
      601 tccgctggca ccccaggcag aagctgacgc cgctcccttc cggggagatc gagctcgagc
      661 tgccggccgg atcgctcttc gaggcgcgcc gattcgtcct ctcgttcggg aaggacgccg
      721 tcgccctctc gcctcccgag ctcgtcgcgg cgctccgcag cgagacggag gagatggccg
      781 cggcgtaccg cggcgcgaag ggctgaggga ggcgacccgg gaggcgcgcg tccgcggcgc
      841 cctcccgggt cgcgggacgc ccgcggatcg acccgaccgc cgggcgcccc ggcaacctcc
      901 aaaggggcgc gccgtaccat cccggcatgc ccgacaggga gctccggccg gccgcgcccc
      961 ccgcctgggg cctcctcctc gcgatcgccg gagcgaggct cgcgctccac gcggccctcg
     1021 cggaccgcta cggctacttc cgggacgagc tctacttcct cgactgcggg cggcacctcg
     1081 cctggggcta cgtcgacgcg gccccggcga tcggcctcta cgcgaaactc gcgctcctcc
     1141 tcggcggctc gctcccggcc ctccggctcc tcgccgggct cgccggagcc ggcgtcgtcc
     1201 tcctcaccgg ccttctcgcg tggcggctcg gcgggagccg ggccgcgcag gggctcgcgg
     1261 ccgtcgcggc gcttgcgacg ccgatcttcc tcgcgatcgc ctccctcttc tcgatgaacg
     1321 tcgtcgagcc tctcttctgg acgggctgcg cgatcgtcct cgtccgcctc gccaacggcg
     1381 cctccccgcg cctctggctc ctcctcggcc tcctcgccgg gctcggcctg ctcaacaagc
     1441 actcgacggc gttcttcggc gtcgcggtcg cggcggggat cctcctctcg cccgcgcgcc
     1501 gcgccctggc gacgccctgg ccgtgggccg gcgccggcgt cgcgctcctc gtcttcctcc
     1561 cgaacctcct ctggcaggcg acgaacggct tcccgacgct cgaggacctc cgcaacgtcg
     1621 cccgcaccgg gaagaacgtc gtcctcggcc ccggcgagtt cgtcctgacg caggtgctcc
     1681 agcaggggcc gcacctcctg cccctctggc tcggcggcct cgcgtggctc ctcctcgcgc
     1741 ggagcgggcg ctaccgcgcg ctcggcgcgg cgttcgccgt cttcttcgtc ctgatgttcg
     1801 cgatgaacgc gaaggggtac tacctgactc cgatcttccc gatcgtgatc gcggcaggag
     1861 ccgtcggcct cgacgaggcg ctcgcgcggt cgcggcgcct cgcaggccgg gcttggccgc
     1921 gcctcgcggc cacggcgtgg tgcgtcctcc tcacgctccc cgcggcgccg atggtcctcc
     1981 cgctcctcga cccgcccgtg ttcgtcgact acacgcggcg cctcggcttc acgccgccga
     2041 gggccgaggt cgcgcacgag gggccgctcc cgcagatgtt cggagaccag cacggctggc
     2101 cggagctcgt cacggaggtc gcgagggtct ggaatggcct gacgcccgag gagcgcgccc
     2161 gcggcgcgat cttcgcgaac aactacggcg aggccggagc gatcaacctc ctcgggccgg
     2221 cgcagggcct cccgcgcgcg atctccgccc accagaacca cttcttttgg ggaccccgcg
     2281 gcttccgcgg cgacgtcctc gtcgtcctcc aggacgaccg cgtgagcctc gagcggctct
     2341 gctcgtccgt agaggaggcc ggccgacacg cccacccgtg ggggatggcg gaggagaacg
     2401 ggccgatctg ggtctgccgc ggcctgaaga cgccgcttca ggagctctgg ccccgcctga
     2461 agcactggaa ctgagcaccg gccgtgcggc ccgggagacc tgctaaggtg cgcgacgcgc
     2521 caagcgaccc agcgagagga cttccggctt cggatcacgt atgagcagca tcctcgtcac
     2581 cggcggcgcc ggtttcatcg gttcccacac aacgctcgag ctccttcgga ccgggcacga
     2641 cgtcgtcgtc ctcgacagcc tcgtcaactc ccggcgcgag gccgtcgacc gcgtcggcgc
     2701 cctggccggg cgcgaggtcc cgttcgtcga ggccgacgtc cgcgaccgcg cggccctcga
     2761 cgcgcatttc gcgcggcacc cggtccgggc cgtgatccac ttcgccggcc tgaaggccgt
     2821 cggggaatcc gtcgagaagc cgctcgccta ctgggacgtg aacgtctgcg ggagcgtggc
     2881 gctcctcgac gcgatgaagc aggccggcgt caagacgctc gtcttcagct cctcggcgac
     2941 ggtctacggc gacccggcgt ccgtgccgat ccccgaggac gccccggtcg gcacgccgtc
     3001 gaacccgtac ggccggacga agctcgtcgt cgagcagctc ctcgccgatg tcttcgcgtc
     3061 ggacccggag tggaggatcg cccggctgcg ctacttcaac ccggtcgggg ccgacgcgag
     3121 cgggcggatc ggagaggacc cgcggggcct cccgaacaac ctcatgccct acgtcgcgca
     3181 ggtcgcggtc ggccgccggg aggcgctgtc ggtcttcggc gacgactatc cgacgccgga
     3241 cgggaccggc gtccgcgact acatccacgt caccgacctc gcggtcggcc acctcgccgc
     3301 cctcgcgaag ctcgaggaag ggcccggcct tcggacctgg aacctcggca ccgggcaggg
     3361 ccagagcgtc ctcgaggtcg tccgggcgtt cgaggaggcc tccggccgga aggtcccgta
     3421 ccgggtcgtc cc
//
LOCUS       DAOONL010000243         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_33461_length_3422_cov_17.889516, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000243 DAOONL010000000
VERSION     DAOONL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33461_length_3422_cov_17.889516"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3049)
                     /locus_tag="PLP50_17560"
     CDS             complement(<1..3049)
                     /locus_tag="PLP50_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPA53403.1"
                     /translation="MRVFDFRDQLIADYAAYVRSFIEIRDARIRAYVDWLLEEGLLWP
                     QPLIQLNPSFEPGRWVDDLVADGVLHSLCAKVFRRDKDREEVKGEGRRLRLHRHQEEA
                     ILTARKGESYVLTTGTGSGKSLAYIVPIVDHVLRRGSGKGIQAIVVYPMNALANSQAG
                     ELRKFVCEGFPNGIGPVTFARYTGQESDEERQKILDNPPDILLTNFVMLELLLTRPFE
                     RKLIDAAQGLRFLVLDELHTYRGRQGADVALLVRRVRDRMTATELQCVGTSATLAAGG
                     TWADQSAEVARVASLLFGTTVKPASIIGETLRRATKPRPADDPAWLAELRTTVETPGH
                     GLPTDYDTFLAHPLATWLETTFGTTEEQGSGRLVRIRTPRSVLGDEGAARDLAGLTGF
                     DEELCADAIRTGLLSGYLARHPETGDPAFAFRIHQFISRGDTVFATVEPEAERHVTVH
                     GQRFVPGDRSRVLLPLVFCRECGQEYYCVRRREDDSGASFEPREPSDRAKDGHSEAGF
                     LFFSSENPWPTDDASVLDRLPEDWVEERPSGWRVKPHKKKALPRPVHVRPDGSESGDA
                     EGHPCHFVTAPFRFCLNCGVAYGSRQISDFGKVGALGSEGRSTATTILSLSAIRQIRA
                     QKTLPEKAQKLLSFTDNRQDASLQAGHFNDFIEIGVLRAGLYRAVVDAGPGGIEHHDL
                     TQKVFQALGLPFAAYARDPDVRFLARTDTERAFRDILGYRIYRDLKRGWRITLPNLEQ
                     CGLLKIRYRSLDELVADEETWSGADPALAALRPDQREEILHTLLDFMRRELALKVDFL
                     EPHRQDSLRQLSNQRLVEPWAIDESDVLEQARVLYPRSVQEDDRRDSVFLSSRGGFGQ
                     FLGRASVLGDRWKALDQAGREGLIRALLEALRVAGIVERVVEADGPDDVPGYQLNAGA
                     LVWQAGDGSASAHDPIRVPHPPKDGGRTNRFFIDFYRRLALDVHDLRAREHTAQVPAD
                     EREKREKLFREGRLPVLYCSPTMELGVDISELNVVNMRNVPPTP"
BASE COUNT          478 a         1093 c         1326 g          525 t
ORIGIN      
        1 ccggggtggg cgggacgttc cgcatgttga cgacgttcag ctcggagatg tcgacgccga
       61 gctccatcgt cggggagcag tagaggacgg gaagccgccc ctcccggaag agcttctcgc
      121 gcttctcgcg ctcgtccgcg ggcacctggg cggtgtgctc gcgggcgcgg aggtcgtgga
      181 cgtcgagcgc gaggcgccgg tagaagtcga tgaagaagcg gttcgtccgg ccgccgtcct
      241 tcggcgggtg agggacgcgg atcgggtcgt gggccgaggc gctgccgtcg cccgcctgcc
      301 agacgagggc gcccgcgttg agctggtagc cggggacgtc gtccggcccg tcggcctcga
      361 cgacgcgctc gacgatgccg gcgacccgga gcgcctcgag gagcgcccgg atgagcccct
      421 cgcgcccggc ctggtcgagc gccttccacc gatcgccgag gacggaagcc cggccgagga
      481 actggccgaa gccgccgcgg gaggagagga agacggagtc gcgccggtcg tcctcttgaa
      541 cggagcgcgg gtagaggacg cgcgcctgtt cgaggacgtc gctctcgtcg atggcccacg
      601 gctcgacgag ccgctggttg ctgagctggc ggagggagtc ctggcggtgg ggctcgagga
      661 agtcgacctt gagcgcgagc tcgcggcgca tgaagtcgag gagggtgtgg aggatctcct
      721 ccctctggtc gggccggagg gccgcgaggg ccgggtcggc cccgctccac gtctcctcgt
      781 cggcgacgag ctcgtcgagc gaacggtagc ggatcttgag gaggccgcac tgctcgaggt
      841 tcgggagggt gatgcgccag ccgcgcttga ggtcccggta gatgcggtag ccgaggatgt
      901 cgcggaaggc tcgctcggtg tcggtgcggg cgagaaacct aacgtcgggg tcgcgggcgt
      961 aggccgcgaa cggcagccca agcgcctgga agaccttctg cgtcaggtcg tggtgctcga
     1021 tgccgccggg gccggcgtcg acgacggccc ggtagaggcc ggcgcggagg acgccgatct
     1081 cgatgaagtc gttgaagtgc ccggcctgga gggaggcgtc ctgccggttg tcagtgaagc
     1141 tgaggagctt ctgcgccttc tcggggagcg tcttctgggc gcggatctgc cggatggccg
     1201 agaggctgag gatcgtcgtc gcagtgctgc gcccctccga gccgagggcg ccgactttgc
     1261 cgaagtcgga gatctgccgg gagccgtaag cgacgccgca gttgaggcag aagcggaacg
     1321 gcgccgtgac gaagtggcag ggatggccct cggcgtcgcc ggattcgctc ccgtcggggc
     1381 ggacgtgcac gggccgcggg agcgctttct tcttgtgggg cttcacgcgc cagccggagg
     1441 gccgctcttc gacccagtct tccgggaggc ggtcgaggac ggaggcgtcg tcggtcggcc
     1501 aggggttctc ggagctgaag aagaggaacc cggcctcgga gtggccgtcc ttcgcgcggt
     1561 cggagggctc gcggggctcg aacgaggcgc cgctgtcgtc ctcgcgcctc ctcacgcagt
     1621 agtactcctg cccgcactcg cggcagaaga cgagcgggag gaggacgcgg gagcggtctc
     1681 cggggacgaa ccgctggccg tggactgtga cgtgccgctc ggcctcgggc tcgaccgtgg
     1741 cgaagacggt gtctcccctg ctgatgaact ggtggatgcg gaaggcgaag gctgggtcgc
     1801 ccgtctcggg gtggcgggcg aggtagcccg agaggagccc ggtgcggatg gcgtcggcgc
     1861 agagctcctc gtcgaacccc gtcaggcccg cgaggtcgcg cgccgcgccc tcgtcgccga
     1921 ggacgcttcg gggcgtgcgg atccgtacga gccggcccga gccctgctcc tccgtcgtcc
     1981 cgaaggtggt ctcgagccag gtggcgagag ggtgcgcgag gaacgtgtcg taatccgtcg
     2041 ggagtccgtg tcccggcgtc tcgacggtcg ttcgcagctc ggcgagccag gcggggtcgt
     2101 cggccgggcg cggcttcgtg gcgcgcctca gcgtctcccc gatgatcgac gccggcttca
     2161 ccgtcgtccc gaagaggagc gaggcgaccc gtgcgacctc ggcgctctga tcggcccacg
     2221 tgccgccggc ggcgagcgtc gcggacgtgc cgacgcactg gagctccgtt gccgtcatcc
     2281 ggtcgcggac gcgacggacg agaagggcga cgtcggctcc ctgccgcccg cggtaggtgt
     2341 ggagctcgtc gaggaccagg aaacggagcc cctgcgcggc atcgatgagc ttccgctcga
     2401 acggtcgcgt gaggagcagc tcgagcatga cgaagttcgt caggaggatg tcgggcgggt
     2461 tgtcgaggat cttctggcgc tcctcgtccg attcctgccc cgtgtagcgc gcgaaggtga
     2521 ccgggccgat gccgttcggg aagccctcgc agacgaactt ccgcagctcc cccgcctggc
     2581 tgttggccag cgcgttcatc gggtagacga cgatcgcctg gatccccttg ccgctccccc
     2641 ggcggaggac gtggtcgacg atggggacga tgtatgcgag gctctttccg gagcccgtcc
     2701 cggtcgtcag gacgtagctc tcgcccttgc gggccgtgag gatcgcctcc tcctggtggc
     2761 ggtggaggcg gaggcgccga ccctcacctt tcacctcttc gcggtccttg tcgcggcgga
     2821 agaccttcgc gcagagggaa tgaaggacgc cgtcggcgac gagatcgtcg acccaccggc
     2881 ccggctcgaa tgaggggttc agctggatga gaggctgggg ccagaggagg ccctcttcca
     2941 gaagccagtc gacgtacgca cggatgcggg cgtcgcggat ctcgatgaag ctccggacgt
     3001 aggccgcgta gtccgcgatg agctgatccc gaaaatcgaa gaccctcact cgctcctccc
     3061 gccgggggcc gaagcccgga gaccgtcgtg gcgcgagagg aggcgtccgt cgaggggccg
     3121 ctcgactcga gccgggcgaa ggcatgaggc gccgggacga atccgccgcc agacggattc
     3181 tgcggtgtgg ggtcgcgatt gggaatgatg cggggagatc agcgggcggc gattacgcgt
     3241 ggcgtcgcgt ctcacccgtt cgggggatga cagccggcgc cgatccgtga tcggagcggc
     3301 gccggacggc gtcatgctcc gctcccgcct tccgagccgc cctccttccc ttcctcctcg
     3361 cccccgaccc cgtagtcgaa gccgtagacg tcggtgtacc agacggggcc gcaggccatg
     3421 cc
//
LOCUS       DAOONL010000244         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_33716_length_3403_cov_15.850358, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000244 DAOONL010000000
VERSION     DAOONL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33716_length_3403_cov_15.850358"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLP50_17565"
     CDS             complement(<1..893)
                     /locus_tag="PLP50_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPA53404.1"
                     /translation="MISLQIEKTFHRAWGALEPEPRAQVGDFLRKFLEDPSQPSLSLE
                     RLDRIKNKGLWSARVTKGYRAIFSQNGDQYALLYVDRHDDAYAWAKAKRAERNDRTGE
                     LQIFVSPEVVEVPPVSTAAKAATKAKGLFDDAEDEYLLSLGLPADWLPTIRKVKEPRQ
                     LESISKDLPEELAERLERVSRGELVTPPPRAPEGPAAFETPESRRRYFVIHDASEIEE
                     LRDKPIEAWMRYLHPSQRALVEADFSGPAKVTGAAGTGKTVVAMHRARLLAAKGKRVL
                     LTSFVGTLCKNLERSMGVLCKG"
     gene            complement(890..>3403)
                     /locus_tag="PLP50_17570"
     CDS             complement(890..>3403)
                     /locus_tag="PLP50_17570"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPA53405.1"
                     /translation="VDISELNVVNMRNVPPTPANYAQRSGRAGRSGQPALVFSYCAAG
                     SPHDQYFFRRPSQMVAGAVAPPRLDLANEDLVRAHVHAVWLAETGVSLGKSLKDVLDT
                     AGDEPSLKLQSGIRNDVENPGALDRARTRARAVLENLRPELDDAGWYTEGWLDRELDQ
                     VVGRFDAACNRWRGLFRAALGQAKVQNRIIQDASASPEARRMAERLRAEAESQLKLLV
                     DVEKIVQSDFYSYRYFASEGFLPGYNFPRLPLSAYIPGRRTRDEDEFLSRPRFLAISE
                     FGPRAVVYHEGSRYVINKVILPVREEAGLTGEAKICRHCGWLHSLATGAGPDLCARCG
                     TELPGGLKGLLRLQNVSTRRKDRINSDEEERLRLGYELKTAVRFSDHGEHVTRFASVR
                     VDGGESVRLDYGPAATLWRLNLGWIRRTDPNQLGFVLDVERGYWAKNDDVDPTDSDDP
                     MSTRRERVVPYVEDRRNCLLVTFADEKPANVMASLQAALKNAIQIQYQLEESELAAEP
                     LPTRKERRHLLLYEAAEGGAGVLRRLVDEPSAFGAVVRRAQELCHFDPDTGEDLRRAP
                     TSKDDCEAACYDCLLSYGNQPDHRLLDRRAIRDLLASLAGATVELSAAGVPANVHLEE
                     LSRKAGSELERRWLDWMHVRQLRLPSRGQVLIADAGTRPDFLYDEHALAVYVDGTPHD
                     FPERQERDRKQETALEDLGWSVVRFGHRDDWGAVVARYPEVFGTPVEAASAAPASAQG
                     LDLDLFPAAWRGVMADLAAQDGVMVTAGGDVVSEGRVVGRTIAAVQRGGRSVTIVQSD
                     APGAAGAVEALEASGKNVVTLSPTPGAGASVLAALTEGK"
BASE COUNT          467 a         1097 c         1312 g          527 t
ORIGIN      
        1 cccttgcaga ggacgcccat gctccgctcg aggttcttgc agagggtgcc gacgaagctc
       61 gtgaggagaa cgcgcttccc cttcgcggcc aggaggcggg cacggtgcat ggcgacgacg
      121 gtcttgcccg tgcccgcggc gcccgtgacc ttcgcggggc cggagaagtc ggcctcgacg
      181 agcgcccgct ggctcgggtg gaggtaccgc atccaggctt cgatcggctt gtcgcgcagc
      241 tcctcgatct cggaggcgtc gtggatgacg aagtagcggc ggcgggattc gggagtttcg
      301 aaagcggcgg ggccctcggg agcgcgcggc ggaggggtga cgagctcgcc gcggctgacg
      361 cgctcgaggc gctcggccag ctcttccggg aggtccttcg agatcgactc gagctgccgc
      421 ggctccttca ccttgcggat cgtggggagc cagtcggccg gcagtccgag gctcaggagg
      481 tactcgtcct cggcgtcgtc gaagaggcct ttcgctttcg tcgccgcctt cgcagccgtg
      541 gagacggggg ggacctcgac gacctcggga gagacgaaga tctgaagctc gccggtgcgg
      601 tcgttccgct ccgcacgctt cgccttggcc caggcgtagg catcgtcgtg ccggtcgacg
      661 tagagaaggg cgtactgatc cccgttctga ctgaatatcg cccggtagcc cttggtgact
      721 cgggccgacc agaggccctt gttcttgatg cggtcgagac gttcgaggct caggctcggc
      781 tggctcgggt cttcgagaaa cttcctgagg aagtcgccca cctgcgctcg gggctccggc
      841 tcgagcgcgc cccaagcccg atggaacgtc ttctcgatct ggaggctgat catttcccct
      901 ccgtgagggc tgcgaggacg gaagcgcccg ctccgggtgt cggcgacagc gtgacaacgt
      961 tcttccccga cgcttcgagg gcctcgaccg ctcccgccgc gcccggggca tcactctgaa
     1021 cgatcgtcac cgatcggccg ccacgctgta cggccgcgat cgttcggccc accactcgtc
     1081 cctccgaaac gacgtcgcct cccgctgtga ccatgacgcc gtcctgcgcg gccagatcgg
     1141 ccattacgcc gcgccaggcc gccgggaaga ggtcgaggtc gaggccctgc gccgacgcag
     1201 gtgccgctga tgccgcctcg accggcgtcc cgaagacctc ggggtacctc gcgacgacgg
     1261 caccccagtc gtcccggtgc ccgaagcgga cgacggacca gccgaggtct tcgagggccg
     1321 tttcctgctt ccggtcgcgc tcctggcgct ccgggaagtc gtgcggggtg ccgtccacgt
     1381 agaccgcgag ggcgtgctcg tcgtagagga agtcggggcg ggtgcccgcg tcggcgatga
     1441 ggacctgccc gcgcgaagga aggcggagct ggcggacgtg catccagtcg agccagcggc
     1501 gctcgagctc ggaaccggcc ttgcgcgaca gctcctcgag atggacgttg gccgggacgc
     1561 ccgcagccga gagctcgacg gtcgccccgg cgagcgaggc gaggaggtcg cgaatcgccc
     1621 gccggtcgag aaggcggtgg tcgggctggt tgccgtagct gaggaggcag tcgtagcagg
     1681 cggcctcgca gtcgtccttc gaggtggggg cgcggcggag gtcttcgccg gtgtcgggat
     1741 cgaagtgaca gagctcctgc gcgcggcgga cgaccgcacc aaaggccgag ggctcgtcca
     1801 cgaggcggcg gaggacgccc gctccccctt cggccgcctc gtagaggagg aggtggcggc
     1861 gctccttgcg cgtcgggagc ggctcggccg cgagctcgct ctcctcgagc tggtactgga
     1921 tctggatggc gttcttcagg gcggcctgca gggaggccat gacgttcgcg ggcttctcgt
     1981 cggcgaaggt gacgaggagg cagttgcgcc ggtcctcgac gtaggggacg acccgctcgc
     2041 gccgcgtgga catcgggtcg tccgagtcgg tcgggtcgac gtcgtcgttc ttcgcccagt
     2101 agccgcgctc gacgtcgagg acgaagccga gctggttcgg gtcggtccgc cggatccagc
     2161 cgaggttgag gcgccagagg gtggcggccg ggccgtagtc gaggcggacg gactctcctc
     2221 cgtcgacgcg caccgaggcg aaccgcgtga cgtgctcgcc gtggtcggag aagcggacgg
     2281 ccgtcttgag ctcgtagccg aggcggaggc gctcctcctc gtcggagttg atccggtcct
     2341 tccttcgggt ggagacgttc tggaggcgga ggagcccctt caggccgccc ggcagctccg
     2401 tgccgcagcg tgcgcagagg tcggggccgg cgccggtcgc gagcgagtgg agccagccgc
     2461 agtggcggca gatcttggcc tcgccggtga ggccggcctc ctcgcggacc gggaggatga
     2521 ccttgttgat cacgtagcgc gagccctcgt gatagacgac ggcccgcggc ccgaactcgg
     2581 aaatggcgag gaagcgcggc cgcgagagga actcgtcctc gtcccgcgtg cgccgcccgg
     2641 ggatgtaggc cgagagcggc aggcgcggga agttgtagcc cgggaggaag ccctcgctcg
     2701 cgaagtagcg atacgagtag aagtcggact ggacgatctt ctcgacgtcg acgaggagct
     2761 tgagctgcga ttcggcctcg gcgcggaggc gctcggccat gcggcgggcc tcgggcgagg
     2821 cggaggcgtc ctggatgatc cggttctgga ccttcgcctg gccgagcgcg gcgcggaaga
     2881 ggccgcgcca gcggttgcag gcggcgtcga agcggccgac gacctggtcg agctcgcggt
     2941 cgagccaccc ctccgtgtac cagccggcgt cgtcgagctc cgggcggagg ttctcgagga
     3001 cggcgcgggc ccgcgttcgc gcccggtcga gggcgccggg gttctcgacg tcgttccgga
     3061 tgccggactg gagcttgagc gaaggctcgt cgcccgcggt gtcgaggacg tccttgagcg
     3121 acttgccgag ggagacgccg gtctccgcga gccagacggc gtggacgtga gcgcggacga
     3181 ggtcctcgtt ggcgaggtcg aggcgggggg gcgcgacggc gccggcgacc atctgggagg
     3241 ggcgccggaa gaagtactgg tcgtgcgggc tgccggcagc gcagtagctg aagacgagcg
     3301 cgggctggcc gctccggccc gcccggccgc tgcgctgggc gtagttggcc ggggtgggcg
     3361 ggacgttccg catgttgacg acgttcagct cggagatgtc gac
//
LOCUS       DAOONL010000245         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_34407_length_3352_cov_12.505611, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000245 DAOONL010000000
VERSION     DAOONL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34407_length_3352_cov_12.505611"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLP50_17575"
     CDS             complement(<1..826)
                     /locus_tag="PLP50_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53406.1"
                     /translation="MFLALLVPIGITAARKIATTGQTGLLLTPEESGFRVRVPGTAAS
                     TGLVPGDLLLLVDGNEARSLPDPVGAIEGDVELTVLRDGQLLRLAGRVDASPWDTRYF
                     LLLFVGAAFVVAAGAVLRTAPSEADPTSAFLFSGFAFSAAAILVLTPAPPVDALFAFA
                     TLVEDVARAFLPAFLLAFVFRFPRKAAHAPDALFFAPAVILAALSIATYLDPPAADVD
                     STAFVLRLDRAQQAAMLLGVVVAVVRLVALASRRLDLLAEKQVRFLLAGTAGGLLPV"
     gene            complement(1032..2906)
                     /locus_tag="PLP50_17580"
     CDS             complement(1032..2906)
                     /locus_tag="PLP50_17580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA53407.1"
                     /translation="MRRLARPLRPAVLAGCFAAFFAAGAEAAPGAATAPLSGILRADG
                     APLAGVSLVVRGLTGAATSVVRVLKTDAEGTFCLADARPGVYSILAAVPGFRSATAQV
                     LHRASGESLSFVRLDLDRDRRGVLPAGPGGALDPWTARAVLGGDVLRDEGPVETPAAA
                     SGAGRAPAASVVASAAVPLPLRGTVRSLQGFAAEGGESLSQTALDIRGSLGKDATWGL
                     SGEFDRLTAAGGEQLGRTALVAVDVLPARGHSIHVSSRRSELPSFDHRDARLDAHSVD
                     WAAEPALGSRASVSARLVSHRNLEPALPTPLFRGEGRALAVDARYRSEVGEGRFVRLH
                     VGYRSDLVDGTVAHEARGGQEARVGGAAGIRLLDALLVEAGGTGDYSEASRGLTPEIT
                     VSLEALAGVTVYGFASRRFEDRDPNVPLYGLVGIDAADLVRATRSHYRAGTRLESGDG
                     TTRLDLEASRREISEAFQLLLDSDLIDRVDALYLFPGDVTDELSGSFSFVLGPGLAAR
                     LAVTAGRARGGGELPGTLLNEARYQVSSARVDVLPSGTAVSVRYRLLEQELGRAAAFR
                     NGRESVDVTLAQEIPIPVLRALGSRWQALFSVALENRSEGDSPVRPNRQMSGGLSLTF
                     "
     gene            complement(3053..>3352)
                     /locus_tag="PLP50_17585"
     CDS             complement(3053..>3352)
                     /locus_tag="PLP50_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPA53408.1"
                     /translation="KHLSAGCYAGIATHDEACVQRALATIDRLKLSPDRYEFQMLLGV
                     DPLLRRTILAAGHRLRVYVPYGKDWYKYSVRRLKENPSIAGHTVRSILGIGPAQS"
BASE COUNT          542 a         1114 c         1318 g          378 t
ORIGIN      
        1 ccacgggcag gaggcccccg gccgtcccgg cgaggaggaa gcggacctgc ttctccgcga
       61 ggaggtcgag gcggcgggag gcgagcgcga cgaggcggac gacggcgacg acgacgccga
      121 gcagcatcgc cgcctgctgc gcgcggtcga gacggaggac gaacgccgtg gagtcgacgt
      181 cggcggcggg cgggtcgagg taggtcgcga tcgagagcgc ggcgaggatg accgcggggg
      241 cgaagaagag cgcgtcggga gcgtgagcgg ccttgcgcgg gaaccggaag acgaaggcga
      301 ggaggaacgc cgggaggaag gccctcgcga cgtcctccac gagcgtcgcg aacgcgaaga
      361 gggcgtcgac cgggggcgcg ggcgtgagga cgaggatcgc cgccgccgag aacgcgaagc
      421 cggagaagag gaacgcgctc gtcgggtcgg cctcgctcgg cgcggtccga aggacggctc
      481 ccgcggccac gacgaacgcg gccccgacga agaggagcag gaagtagcgg gtgtcccacg
      541 gcgacgcgtc gacccggccc gcgaggcgga ggagctgccc gtcccgcagg acggtgagct
      601 cgacgtctcc ttcgatcgcg ccgacagggt cagggaggga gcgcgcctcg ttcccgtcga
      661 cgaggagcag gaggtcgccg ggaacgagcc cggtcgaggc ggccgtcccg gggacccgaa
      721 cccgaaagcc gctctcctcg ggggtgagga ggagccccgt ctgtcccgtc gtggcgatct
      781 tccgggcagc ggtgatgccg atgggcacga ggagagcgag gaacactccg aggaatcccc
      841 atcggagaat tcgagtgctc cgccttcgca ggttcctcat cccacaaaag cgcaggcaaa
      901 gggagtgcca gggattcgga tcgttggagc ggtggaaact ggctccggag aggtgtttag
      961 gcgacaaccg tgacgaattg cggcgcccgc gaccgcaaaa gttgaatcga aatccaaaaa
     1021 acccgggcgg ctcagaacgt gagcgagaga ccgccggaca tctgccggtt cgggcgaacg
     1081 ggcgagtcgc cttcgctccg attctcgagc gccacggaga agagcgcctg ccaccgcgat
     1141 ccgagggcgc ggaggaccgg gatcgggatc tcctgggcga gtgtcacgtc gaccgactcc
     1201 cggccgttcc ggaaggcggc ggcgcgtccg agctcctgct cgagaagcct gtagcggacc
     1261 gagaccgccg tcccgctcgg caggacgtcg acacgcgcgc tggagacctg gtaccgggcc
     1321 tcgttcagga gcgtacccgg cagctctccg ccgcctcggg cacgtcccgc cgtgaccgcg
     1381 agacgggccg ccaggccggg gccgaggacg aacgagaacg agccggacag ctcgtccgtc
     1441 acgtcgccgg ggaagagata gagggcgtcg acccggtcga tgaggtcgga atcgaggagg
     1501 agctggaacg cctcggagat ctcccggcgg ctcgcctcga ggtcgaggcg ggtcgtcccg
     1561 tcgcccgact cgagccgcgt gccggcccga tagtgggagc gggtcgccct cacgaggtcg
     1621 gcggcgtcga tgccgacgag cccgtagagc gggacgttgg ggtcgcggtc ctcgaaacgc
     1681 cgtgaggcga agccgtaaac ggtgacgccg gcgagggcct ccagcgagac ggtgatctcg
     1741 ggcgtcagcc cgcgagaggc ttcggagtag tcgcctgtcc ccccggcctc cacgagaagg
     1801 gcgtcgagga gccggatccc ggcggcgccg ccgacccgcg cctcctggcc gccgcgcgcc
     1861 tcgtgcgcga ccgtcccgtc gacgagatcc gagcgatagc cgacgtggag gcggacgaag
     1921 cgtccttccc cgacctcgct ccggtacctc gcgtcgaccg cgagcgcccg cccctcgccg
     1981 cggaagagcg gtgtcggaag cgccggctcg aggttccggt gcgacacgag ccgggccgag
     2041 acggaggcgc gtgagccgag agccggctct gcggcccagt cgacggagtg cgcgtcgagt
     2101 cgcgcgtcac ggtggtcgaa ggagggaagc tcgctgcgcc ggctcgagac gtggatgctg
     2161 tggccgcgcg cgggaaggac gtcgaccgcg acgagggccg tccgccccag ctgctctcca
     2221 ccggccgcgg tcagccggtc gaactcgccc gagaggcccc acgtcgcgtc cttgccgagg
     2281 ctgccgcgga tgtcgagcgc cgtctgggag agcgactcgc ccccttcggc ggcgaatccc
     2341 tggagcgagc ggacggtccc gcgaagaggg agcgggacgg ctgcggaggc gacgaccgaa
     2401 gcggccggtg cacgtccggc cccgctcgcc gccgccggag tctcgacggg cccttcgtcg
     2461 cgcaggacgt cgccgccgag gaccgcccgc gcggtccagg ggtcgagcgc cccgcccggg
     2521 ccggcgggca ggacgccgcg acggtcgcgg tcgaggtcga gccggacgaa cgagaggctc
     2581 tcgccgctgg cgcggtggag cacctgggcc gtggccgagc ggaatcccgg cacggccgcg
     2641 aggatcgagt agacgccggg acgggcgtcc gcgaggcaga aggtcccctc tgcgtcggtc
     2701 ttcaggacgc ggacgacgga cgtcgcggcg ccggtcagcc cccggacgac gagcgagacg
     2761 ccggcgagag gtgctccgtc ggcgcgaagg atcccggaga ggggtgccgt cgccgctccg
     2821 ggggcggcct ccgcccccgc ggcaaagaaa gccgcgaaac agccggcgag tacggccgga
     2881 cgcaggggtc gcgcgaggcg cctcacaggt tcgactccga ctcccgagag ggcgtcatgt
     2941 tgaatcatgg tccccccgga ccggtggagt caaccattcc ggcccggaaa cgggtgaccg
     3001 gccccggggg gccgaccgcc gtcggggctc gggcgggccc ccggggcaga ggtcaggatt
     3061 gcgcgggacc gatccccagg atcgaccgga ccgtgtgccc ggcgatcgag gggttctcct
     3121 tcagccggcg aaccgagtac ttgtaccagt ccttcccgta ggggacgtag acgcggaggc
     3181 gatgtccggc cgcgaggatc gtcctccgga ggagggggtc gacgccgagg agcatctgga
     3241 actcgtaccg gtcaggggag agcttcagcc ggtcgatcgt cgcgagcgcc cgctggacgc
     3301 acgcctcgtc gtgcgtcgcg atgccggcgt agcagccggc cgagaggtgc tt
//
LOCUS       DAOONL010000246         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_34566_length_3340_cov_10.822222, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000246 DAOONL010000000
VERSION     DAOONL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34566_length_3340_cov_10.822222"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLP50_17590"
     CDS             <1..269
                     /locus_tag="PLP50_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53409.1"
                     /translation="AIPTVCCKKFSMKTCCCPCNMAMTIWGLSNYMLVVKGADAAQLK
                     TAVLDWVKFIGPAGYTGDACFKGGCNRPFAKNGCGGMDHKSVIF"
     gene            1045..1464
                     /locus_tag="PLP50_17595"
     CDS             1045..1464
                     /locus_tag="PLP50_17595"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPA53410.1"
                     /translation="MRRLLATFAALAFSLWPLAAQANQGHAHAHGQDAVLGTIAKVEG
                     DRLEVADPDGKSVIVSLTRTTMYLKGSEEAKKADLKPGLSVAVETANGKAGLEAKVVR
                     IGPADGAVWACPMHPEVTGSGPAKCPKCGMFLEKKKA"
     gene            1505..2026
                     /locus_tag="PLP50_17600"
     CDS             1505..2026
                     /locus_tag="PLP50_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53411.1"
                     /translation="MARLLFGALLVSGALEGQEPPRLDTPVAPSAAGEPANPGVSRLE
                     RSEGLLAAVRTASAEKDGTALASATAAYVSYLAVLRDEISRAPRVDGPVLPDKLVVGK
                     TVALQATELESLAKAAPRRLRKDVERALAAADGLLETLARGWSAPAPAVRPQAESHAG
                     HGRSTSASSGGHH"
     gene            2081..>3340
                     /locus_tag="PLP50_17605"
     CDS             2081..>3340
                     /locus_tag="PLP50_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPA53412.1"
                     /translation="MNHDEHEHTGHDRSTAGRPQKKDPVCGMSVPADSPNRHEHAGTE
                     FVFCSATCRDRFTRDPGSFLEQAPAATAAPSRVIPGGYTCPMHPEIVRDAPGSCPICG
                     MALELRAPAANAGENAELRDMSRRFWFSVALTVPLLGLAMGDMLPGEPVSALLSARLR
                     TFLELALATPVCLWAAWPFYVRAVQSVRNRSLNMFTLIGLGVSVAYGYSVIATLLPGL
                     FPASFRSASGEVAVYFEAAGVIVTLILLGQVLELRARSQTGAAIQKLLGMAATSARRI
                     NADGSEEDVLLEVVQAGDTLRVRPGEKIPVDGVVLEGASSVDESMVSGEPIPVEKGPG
                     DKVVGATVNGTGALLMRAEKVGAETLLSRIVAMVAEAQRSRAPIQKLADVVAGYFVPI
                     VILISVLTFVAWAIVGPAPRMAHALINA"
BASE COUNT          530 a         1108 c         1182 g          520 t
ORIGIN      
        1 cggcgatccc aaccgtgtgc tgcaagaagt tttccatgaa gacgtgctgc tgcccgtgca
       61 acatggccat gacgatctgg ggactctcga actacatgct cgtggtgaag ggcgccgacg
      121 cggcccagct caagacggcc gtcctcgact gggtgaagtt catcggaccc gccggataca
      181 ccggcgatgc gtgtttcaaa ggtggctgca accggccgtt cgcgaagaac ggttgtggcg
      241 gcatggacca caagagcgtc atcttctagg ggctgagcga tgagactcct tcgaaccctt
      301 ctcctttcga cggcgatggt gagccttccg gtcatggccc aggaacacgt ccacggcgaa
      361 cggggggcgc cggaccaggc gccctcggcg gaccggcccg ttcgggacct ggatgcggcg
      421 cttcgcgacg aagggagcgc cctgcgtgcg ctccgagcga gcatccgctc gcgcgagcgc
      481 gagcgggttg accgatccgt cggggactac gtctcggttc aggatgagct gcgcgtcggg
      541 ctgcgctctg gtggggggga cgaagcgcgt gcgcgagatg cctcgcggat acagaaggtc
      601 tgccgggaga gcctggcaac tctccgtggc ctcgcggacg gggctccgcc cgccctcggg
      661 gaacgtgtcg acgaagcgat cgccgcggcg gagcggacgt tcgggatcgc cgcggagctc
      721 gccggtcagg aggtggaccc tgcaccgagg accagccggg gcggatgtgg catggggagc
      781 tccggccatc gagggcattg accgcgactc ctggcggagc gaacgcggcc gggcctgagg
      841 cgcccgcggt atcgcgagaa gtgaagtgat cgatccgggc ggcgtcgtcc ctgccgatcc
      901 gcggagcgga gcgttccccg gggaggagcg gacgacgccg catctctcta acgtctctct
      961 aaggttcagc ccctatcttc ccgttcaagc gccggcgccg gcgtgtagag cccgccgccg
     1021 gaagcgggac caggagaaaa cgacatgcgc agacttctcg ccaccttcgc ggctctcgcc
     1081 ttctcgctct ggcccctcgc cgctcaggcc aaccagggcc acgcccacgc gcacggccag
     1141 gacgcagtcc tcggtacgat cgccaaggtc gagggcgacc gcctggaggt cgctgacccc
     1201 gacggcaagt ccgtcatcgt cagcctgacg cgcaccacca tgtacctcaa ggggtctgag
     1261 gaggcgaaga aagctgacct gaagcccggg ctgagtgtcg ccgtcgagac ggcaaacggg
     1321 aaggccgggc tcgaagcgaa ggtcgtccgg atcggtcccg ccgacggagc ggtctgggct
     1381 tgcccgatgc acccggaagt gaccggttcc ggtcccgcca agtgcccgaa gtgtggcatg
     1441 ttcctcgaga agaagaaggc ctgaggagcg cccggtgtac cgagtaggag agtcgactcg
     1501 agtcatggct cgcctcctct tcggggcact cctcgtttcg ggcgcgctgg aagggcagga
     1561 gcctccacgg ctcgacacgc cggtcgctcc atctgctgcg ggggagccgg cgaatccagg
     1621 tgtcagccgg ctcgagcgct ccgaagggct tctggccgcc gtccggacgg ccagcgccga
     1681 gaaagacggc accgccctcg cttctgccac ggcagcgtac gtgtcctacc tcgcggtcct
     1741 ccgggacgag atcagccgcg ccccgcgtgt cgatggcccg gtgttgccgg acaagctcgt
     1801 ggtgggaaag acggtagctc tgcaggcgac ggagctcgag tcactggcga aggcggcccc
     1861 gcggcggctg cgaaaagacg tggagcgggc actggccgcc gccgacgggc ttctcgagac
     1921 tctggcgaga ggctggtcgg cgccagcgcc agcggtgcgc ccgcaggccg agtcgcacgc
     1981 gggccacggc cgctcgacgt cggcctcgag cggtgggcac cattgagggc ctctgcacta
     2041 ggctcgtgct gacgacgcgt ccgtcaggtt ggaggcaccc atgaatcatg acgagcacga
     2101 gcacaccggg cacgatcggt cgacggccgg gcggcctcag aagaaggacc ccgtctgcgg
     2161 aatgtcggtg ccggcggaca gcccgaaccg acacgagcac gcgggtaccg agttcgtgtt
     2221 ctgcagcgcg acctgccgcg atcggttcac cagggacccg ggctcgttcc tcgaacaggc
     2281 cccggcggcc acggccgctc cttctcgggt cattccgggg ggctacacgt gccccatgca
     2341 ccctgaaatc gtgcgcgatg ctccgggctc gtgtccgatc tgcggcatgg cgctggagct
     2401 ccgggcaccc gcggcgaacg ccggagagaa cgccgagctc cgcgacatga gccgccgctt
     2461 ctggttctcg gtagcgctta ccgtgccgct cctcggcctg gcgatgggcg acatgctgcc
     2521 gggagaaccc gtgtctgcgc tcttgtcggc gcgcctacgc acattcctgg agctggcgct
     2581 ggcgacgccc gtgtgcctct gggccgcatg gcccttctac gtgcgagccg tccagtcggt
     2641 gaggaaccga agtctcaaca tgttcacact catcggcctc ggcgtgagcg tcgcgtacgg
     2701 ctacagcgtc atcgcgacgc ttctgccggg cctgttcccg gcctccttcc ggagcgcatc
     2761 cggcgaggtc gccgtttact tcgaagcggc cggggtcatc gtcaccctga tcctgctcgg
     2821 gcaggtcctg gagcttcgcg cgcggagcca gaccggtgcc gcgattcaga agctgctcgg
     2881 gatggcagca acatcggcgc gacggatcaa tgctgacggt tccgaagagg acgtgctgct
     2941 ggaggtcgtg caggccggcg acacgctccg ggtgcggcct ggcgagaaga tcccggtaga
     3001 cggtgtcgtc ctcgagggtg cgagctccgt cgacgagtcg atggtgagcg gcgagccgat
     3061 acccgtggag aagggccccg gcgacaaggt cgtcggagct acggtcaacg gcaccggagc
     3121 cctcctgatg cgggccgaaa aggtcggcgc cgagacgctt ctctcgcgca tcgtcgcgat
     3181 ggtcgccgag gcccagcgaa gccgggcgcc gatccagaag ctcgccgatg tcgtggccgg
     3241 ctatttcgtg ccgatcgtca tcctgatctc agtcctcacc ttcgtggcct gggcgatcgt
     3301 cgggccggcg ccccgaatgg cgcacgcgct gatcaacgcc
//
LOCUS       DAOONL010000247         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_34607_length_3337_cov_17.125229, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000247 DAOONL010000000
VERSION     DAOONL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34607_length_3337_cov_17.125229"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="PLP50_17610"
     CDS             complement(<1..1655)
                     /locus_tag="PLP50_17610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPA53413.1"
                     /translation="MSTKYVFVTGGVVSGLGKGIAGASLAALLEARGFKVTLQKLDPY
                     LNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSSTITGKSNSITAGKIYQTVIEK
                     ERRGDYLGKTVQVIPHVTDEIKDCIRRVSADNDVVIVEIGGTVGDIESLPFLEAIRQF
                     RKDVGRGNAINVHVTLVPYIAASDELKTKPTQHSVKELLSIGIRPDVLLCRADRAIPE
                     EMKKKIALFCNVDEPAVITARDVSTIYECPLAFAREGLDQIVLDYLSLPHSERDLTKW
                     DRIVNRWKNPAGEVKIAIVGKYVALGDSYKSLNEALHHGGIENNVKVRLEYVDSEDLE
                     TTGYPESLSRADAVLVPGGFGRRGIEGMLRAIRYARETKVPFFGICLGMQCAVIEFAR
                     NVCGLANANSTEFDANAPHKVIYKLRDLIGIEALGGTMRLGKYPCELAEGSTARRAYG
                     EPLIYERHRHRYEVNQEFLPALREAGLAVTGVSPDRKFVEMVELPDHPWFLGCQFHPE
                     FKSKPTAAHPLFKDFVRAARAHQAAREEGVVQKSAALEPGIAVP"
     gene            complement(1652..2422)
                     /gene="kdsB"
                     /locus_tag="PLP50_17615"
     CDS             complement(1652..2422)
                     /gene="kdsB"
                     /locus_tag="PLP50_17615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPA53414.1"
                     /translation="MIPSRMGAQRFPGKPLHLIAGVPLVVRVLRRAMESRRADVVLVA
                     TDDERIAAAVREAGGTAVMTPSDLPSGTDRVRAAVAGTDAEIVVNVQGDEPLMDPANV
                     DRVIEHLLAHPEVPLATVARPIDDPAVIADPNVVKVVRGDDGRALYFSRSPIPFRRNA
                     EPGLPTWKHLGLYGYRREALDTWTSLPPHPLERAESLEQLRALAAGLVMAVLPAVGDS
                     IGVDTPADAEAVERIILKEAAAREAAGAGTEREAGGER"
     gene            complement(3020..>3337)
                     /locus_tag="PLP50_17620"
     CDS             complement(3020..>3337)
                     /locus_tag="PLP50_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53415.1"
                     /translation="EERWNDALVFLAKILRLNPYDVAARMAVAETYHRGLEEPTKAIL
                     TYEKVIAAAGYDESNPYCASAREGIRELSTSMEEPPFVLNDLVTEEADQEEIGGLPQT
                     IAL"
BASE COUNT          454 a         1124 c         1219 g          540 t
ORIGIN      
        1 gggacggcga tgccgggctc gagcgcggcg ctcttctgca cgacgccctc ctcgcgcgcg
       61 gcctggtggg cacgcgccgc cctcacgaag tccttgaaga gggggtgggc ggcggtcggc
      121 ttcgacttga actcagggtg gaactggcag ccgaggaacc aggggtggtc cggcagctcg
      181 accatctcga cgaacttccg gtcgggcgag acgccggtga cggcgaggcc cgcctcgcgg
      241 agcgccggga ggaactcctg gttcacctcg tagcggtggc ggtggcgctc gtagatcagc
      301 ggctcgccgt aggcgcggcg ggccgtcgac ccctcggcga gctcgcacgg gtacttgccg
      361 agccgcatcg tgcccccgag cgcctcgatc ccgatcaggt cgcgcagctt gtagatgacc
      421 ttgtgcgggg cgttcgcgtc gaactcggtg gagttcgcgt tcgcgaggcc gcagacgttg
      481 cgggcgaact cgatgacggc gcactgcatg ccgaggcaga tgccgaagaa cggcaccttc
      541 gtctcgcggg cgtaccggat ggcgcgcagc atcccctcga tgccccggcg cccgaagccg
      601 cccggcacga ggacggcgtc ggcgcgcgag agcgactcgg ggtagccggt cgtctcgagg
      661 tcctcggagt cgacgtactc gaggcgcacc ttgacgttgt tctcgatgcc gccatggtgg
      721 agcgcctcgt tcagcgactt gtacgagtcg ccgagcgcca cgtacttgcc gacgatggcg
      781 atcttcacct cgcccgccgg gttcttccag cggttgacga tccggtccca cttcgtcagg
      841 tcgcgctcgg aatgggggag cgagaggtaa tcgaggacga tctggtcgag cccctcacgc
      901 gcgaaggcga gcgggcactc gtagatcgtc gagacgtcgc gcgccgtgat gacggccggt
      961 tcgtcgacgt tgcagaagag ggcgatcttc ttcttcatct cctccgggat cgcgcggtcg
     1021 gcgcggcaga ggaggacgtc gggtcggatg ccgatcgaga ggagctcctt gacggagtgc
     1081 tgcgtcggtt tcgtcttcag ctcgtcggag gcggcgatgt acggcacgag ggtcacgtgg
     1141 acgttgatcg cgttcccgcg gccgacgtcc ttgcggaact ggcggatcgc ctcgaggaac
     1201 gggagggact cgatgtcgcc gaccgtcccg ccgatctcga cgatgacgac gtcgttgtcg
     1261 gccgagacgc gccggatgca gtccttgatc tcgtcggtga cgtgcgggat cacctggacg
     1321 gtcttgccga ggtagtcgcc ccgccgctcc ttctcgatga cggtctggta gatcttcccg
     1381 gcggtgatgc tgttcgactt gcccgtgatc gtcgaggaga agcgctcgta gtggccgagg
     1441 tcgaggtcgg tctcggcgcc gtcgtcggtg acgaagacct cgccgtgctg gaagggagac
     1501 atcgtccccg ggtcgacgtt gaggtagggg tcgagcttct ggagcgtcac cttgaagccg
     1561 cgggcctcga gcagggccgc gagggaggcc cccgcgatcc ccttgcccag gcccgagacg
     1621 acccccccgg tgacgaaaac gtatttcgtg ctcatcgctc ccctcccgcc tctctctccg
     1681 taccagcccc ggcggcctcg cgggccgccg cctctttcag gatgatccgc tcgaccgcct
     1741 ccgcgtccgc cggcgtgtcg accccgatgg agtcgccgac ggcggggagg accgccatca
     1801 cgagccccgc ggccaaggcg cggagctgct cgaggctctc cgcccgctcg agcgggtgcg
     1861 gcgggaggct cgtccaggtg tcgagggcct cgcggcgata gccgtagagg cccaggtgtt
     1921 tccaggtcgg gagccccggc tccgcgttcc tccggaacgg gatcgggctc cgcgagaagt
     1981 agagggccct gccgtcgtcg ccccggacga ctttgacgac gttcgggtcc gcgatcacgg
     2041 ccgggtcgtc gatcggcctg gccacggtcg cgagcggcac ctccggatgc gcgaggaggt
     2101 gctcgatcac gcggtcgacg ttcgccgggt ccatgagcgg ctcgtctccc tgcacgttca
     2161 cgacgatctc cgcgtcggtc cccgccacag ccgcgcgcac ccggtcggtc cccgacggga
     2221 ggtcggacgg cgtcatcacc gcggtcccgc ccgcctcgcg gacggcggcg gcgatccgct
     2281 cgtcgtccgt cgcgacgagg acgacgtcag cgcgccgcga ttccatcgcc cgccggagga
     2341 cgcgcacgac gagggggact cctgcaatga ggtggagggg ttttccaggg aagcgctggg
     2401 cgcccatccg ggacgggatc acgacgaccg atctaccagg gtggcgcacg tcgaatggta
     2461 acccatcgtt tcgtggggcc gggtaccatc tccgggtgag cgatcgcccg accgggtcga
     2521 gcctccgggg actcctcggg acgcggccga ggagggcggc ggcgctcctc gtcgggctct
     2581 ccgtcacggg cgccctcggc caggcagcgt ggcgcgccgc gcgcgacccc ctctacccgg
     2641 cccggtggat cggccttcgc gaggccgcgg cgcccgccct tcaggcggaa gtggcgttcg
     2701 tgaaggagtt cgagctcgcc gagccgcttc ggcaccccgt cctcctcgtc gagggggacc
     2761 gggagtggga ggtccgcctc gacggccggc tcgtcgcgac tggaacggga cccggggtcc
     2821 gagagttcca gctgtccgat ccgatctccg ccggccgtca cctcgtcgtg gcggcggccc
     2881 ggcacccgga ggggctcgcg tcgatccgcc tgcgcctcga cggggtcgcc ggaaaaggga
     2941 tcggcgtcgt caccggccgg ggctgggggg ccgacgacga cgcctcgagg atccgggacc
     3001 gggggcgaaa gggagcccgt tacagggcga tggtctgggg gaggccgccg atctcttcct
     3061 ggtcggcctc ctcggtcacg agatcgttga ggacgaacgg gggctcttcc atcgacgtcg
     3121 agagctcacg gattccctca cgagcggaag cgcagtaggg gttcgactcg tcgtaccccg
     3181 ctgcggcgat caccttttcg tacgtcagga tggccttcgt cggctcctcg aggccgcggt
     3241 ggtaggtctc ggcgaccgcc atccgcgcgg cgacgtcata cgggttcagc cggaggatct
     3301 tcgccaggaa gacgagggcg tcgttccagc gctcctc
//
LOCUS       DAOONL010000248         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_34706_length_3330_cov_17.569160, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000248 DAOONL010000000
VERSION     DAOONL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34706_length_3330_cov_17.569160"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1343)
                     /locus_tag="PLP50_17625"
     CDS             complement(189..1343)
                     /locus_tag="PLP50_17625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPA53416.1"
                     /translation="MAVPTRVLPPPKLARFLERHRLLDETSGIAPERFLVTLVRKALD
                     FVPAEAAVLLLDDPVAKEEDRSRNELTVIASAGEIRDPAVGTRVRPVDGIAGFTYARG
                     EQTAVNEIKGARALSREADDVFGSPVRNALGVPVVIESHVCGSLVLVNRIDPAGFDAL
                     DQRLMSLFGETLSLALQNLLDERRAREVARRDSLTALYNDRYFHKKLRLEIDRLSQTP
                     DGDLTLLFLDCDHLKSVNDEHGHLAGSQILREVAFILQRQLAQTTSLPCRYGGDEFTV
                     ILVDCELARGMEIGERIRAAIAEWTFLPMAIGPGEPALNLKGVITMSIGVASYRRHCT
                     PSRPPSEQKNEILKKADAALYAAKEAGKDCVVAARRESGPPSISNIVLKS"
     gene            complement(1353..3212)
                     /locus_tag="PLP50_17630"
     CDS             complement(1353..3212)
                     /locus_tag="PLP50_17630"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPA53417.1"
                     /translation="MSRLPRAAAPLAALAALSVVLAAGPLPAAQEGPEVQVVVDNPRP
                     AADDVVRLTYVFSGPGVGGRLHAPAELPLRNLVSLGPPSSRTQASLINGVFSHTSSLT
                     YFLRPSGTGPAEIGETTFRFGEKKEVTAGAYLLEVGPARREAGARTAMPPQDEDPFDR
                     LLPRRGARPAGVERGLPSAEPILAFVATPSRESAYVGEEVTIAYDLVTEAEVQGIEYV
                     EAPQFPGLWAEDLERPERPQGRKDTFEGRPVARFTLLRKAVSGLTPGTVTLPAAKIRI
                     GVRVALDPFGDPFAMLRPRVVERSTKPITLKVLPIPGRPDFKGPVGQFGVTATVDRKR
                     VPAGEAVTLKVRLAGTGNLRTATETPHLSIPGVKVYPPSSRTLPTRGGTKGGTSAEWD
                     FVLVPTEAGSLVVPPVSFEVFDTAEKKLVARSTTPISITVEAPAPGAVIAEGPAPLSA
                     ASPGAEEAATTLPGEPPTPTPLPSARELAQSTVAVPLWVLVAIPGLLAVAGGATWAVR
                     RRSRALGPTSALLAPEPGETKERAVARVERAVRGHLARRTGVSETAPVGELLEELAGR
                     GVGPELREEVRQLLAQVEFLRFAPQLGDYSDELARLRERAREVLGRVRDERRG"
BASE COUNT          444 a         1128 c         1283 g          475 t
ORIGIN      
        1 gatgaagctc ccgtgcgagc gctcgaggtc catcacgacg tggtagccgt ccacgagcat
       61 gtggcggccg agcgattcga ggacgttctt cgggccgata ccggggatga cgctcgacga
      121 catggtccct ccgccgtccg gccccgtccc ggggccggct ccgcatgtta gcagcgcccc
      181 ggaggcgctc agctcttcag gacgatgttc gagatcgagg gcggaccgct ctcgcggcgg
      241 gccgcgacga cgcagtcctt gcccgcctcc ttggcggcgt agagggcggc gtcggccttc
      301 ttcaggatct cgttcttctg ctcgctcggc ggacggctcg gcgtgcagtg ccgccggtag
      361 gacgccacgc cgatcgacat cgtgatgacg cccttcaggt tcagggcggg ctcgcccggt
      421 ccgatcgcca tcgggaggaa ggtccattcg gcgatcgcgg cgcggatccg ctctccgatc
      481 tccatccccc tcgcgagctc gcagtcgacg aggatgaccg tgaactcgtc gccgccgtag
      541 cggcagggaa gggacgtcgt ctgggcgagc tggcgctgga ggatgaaggc gacctcccgc
      601 aggatctggc tcccggcgag gtgcccgtgc tcgtcgttca ccgacttgag gtggtcgcag
      661 tcgaggaaga ggagcgtcag gtcgccgtcg ggcgtctggg aaaggcggtc gatctccagg
      721 cggagcttct tgtggaagta gcggtcgttg tagagggcgg tgagcgagtc gcgacgcgcc
      781 acctcgcggg cgcgcctctc gtcgaggagg ttctgcagcg cgagggagag cgtctcgccg
      841 aagagcgaca tgaggcgctg gtcgagcgcg tcgaaccccg ccgggtcgat ccggttcacg
      901 aggacgagcg acccgcagac gtgggactcg atgacgacgg gaactccgag agcgttcctc
      961 accggcgatc cgaacacgtc gtccgcctcg cgggagaggg cacgggcccc cttgatctcg
     1021 ttcaccgcgg tctgctcccc gcgcgcgtag gtgaagccgg cgatgccgtc gacgggcctc
     1081 acccgcgtcc cgacggcggg atcgcggatc tccccggccg aggcgatcac ggtcagctcg
     1141 ttccgggacc ggtcttcctc tttcgcgacc gggtcgtcca gaaggaggac ggcggcctcc
     1201 gccggaacga aatcgagcgc cttcctcacg agcgtgacga ggaaccgctc cggggcgatg
     1261 ccgctcgtct cgtccaggag acggtggcgc tccaggaagc gcgcgagctt cggcggagga
     1321 aggacccgcg tcggtacggc cacggctcga ttctagccgc gtcgttcgtc gcggacccgg
     1381 ccgagcacct cccgcgccct ctcgcgcagc cgggcgagct cgtcggagta gtcgccgagc
     1441 tggggcgcga agcggaggaa ctcgacctgc gcgaggagct gtcggacctc ctcccggagc
     1501 tcggggccga ctccccggcc ggcgagctcc tcgaggagct cgccgacggg cgccgtctcg
     1561 gagacgcccg tccggcgggc gaggtggcct cggacggccc gttccacccg cgccaccgcc
     1621 cgctccttcg tctcccccgg ctccggggcg aggagggccg aggtggggcc cagggcacgg
     1681 gagcggcggc ggaccgccca ggtggcgccc cctgcgacgg cgagcaggcc cgggatcgcg
     1741 acgaggaccc agagggggac ggcgaccgtc gactgcgcga gctcgcgcgc cgacgggagg
     1801 ggcgtcggcg tgggcggctc gcccgggagg gtcgtcgccg cctcctccgc gccgggggag
     1861 gcggcggaaa gcggggccgg accctcggcg atcacggcgc ccggggcggg cgcctcgacg
     1921 gtgatcgaga tcggggtcgt cgaccgggcg acgagcttct tctcggcggt gtcgaagacc
     1981 tcgaaggaga cgggagggac gaccaggctg ccggcctcgg tcgggacgag gacgaagtcc
     2041 cactcggcgc tcgtcccgcc cttggtcccg ccgcgggtcg gaagggtccg ggaggacggg
     2101 gggtagacct tcacgcccgg aatcgagagg tgaggggtct cggtcgcggt ccggaggttc
     2161 ccggtgccgg cgaggcggac cttgagcgtg acggcctcac cggccgggac gcgctttcgg
     2221 tcgaccgtcg ccgtgacgcc gaactgcccg accgggccct tgaagtcggg gcgtccgggg
     2281 atcggaagga ccttcagggt gatcggcttc gtcgagcgct cgacgacgcg cggccggagc
     2341 atcgcgaacg gatccccgaa ggggtcgagc gcgacgcgga cgccgatccg gatcttcgcc
     2401 gccgggagcg tcacggtgcc gggcgtgagg ccggagacgg ccttccgcag gagcgtgaaa
     2461 cgcgcgacgg gacgcccctc gaacgtgtcc ttccgcccct gcgggcgctc cggccgctcg
     2521 aggtcttccg cccagaggcc ggggaactgc ggcgcctcga cgtactcgat tccctggacc
     2581 tcggcctcag tcacgaggtc gtaggcgatc gtcacctcct cgccgacgta cgcggactcc
     2641 cggctcgggg tcgcgacgaa cgcgaggatc ggctcggcgg agggaaggcc gcgctcgacg
     2701 ccggccgggc gggctcccct tcgcgggagg aggcggtcga acgggtcctc gtcctgcggc
     2761 ggcatcgccg tgcgggcgcc ggcttcgcgg cgcgcgggcc ccacctcgag gaggtaggcc
     2821 cccgcggtca cctccttctt ttcgccgaaa cggaaggtcg tctcgccgat ctcggcgggg
     2881 cctgtcccgc tcgggcggag gaagtacgtg aggctcgagg tgtgcgagaa gacgccgttg
     2941 atgagggagg cctgggtgcg gctgctcgga gggccgaggg agacgaggtt ccggaggggg
     3001 agctcggccg gggcgtgcag ccggcctccg acgccggggc cggagaagac gtacgtcagc
     3061 ctcacgacgt cgtcggcggc gggacgaggg ttgtcgacga cgacctggac ctcgggtccc
     3121 tcctgcgcgg cggggagcgg gcctgctgcg aggacgacag aaagcgccgc cagcgccgcg
     3181 aggggcgcgg cggcgcgcgg gaggcggctc accagtccct cccggagcgc cgcttcttct
     3241 tctgctcggc gactttcttt cgctgctctt cgaggtcgtt ccgggcgatc gcctggagga
     3301 gctgctcggc cttgtcgcgg ctcatcttcg
//
LOCUS       DAOONL010000249         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_35378_length_3284_cov_18.447817, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000249 DAOONL010000000
VERSION     DAOONL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35378_length_3284_cov_18.447817"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..696)
                     /locus_tag="PLP50_17635"
     CDS             complement(139..696)
                     /locus_tag="PLP50_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53418.1"
                     /translation="MSRRARSLLLAVPILVAAGSVGTAAQELAPSPAAPLAQRESTAR
                     ATEDGLVGGLGFRSLHALMNREAWPLLAASGPLRTGAVDLDAAEPLQAIVLFNAWHLP
                     AAHGPDDLRLRDLRENLFSRERVFGGPWPEYSLPGYVLLLRKADGAWTLVARLSGHFL
                     VEDASGLGLVAVPPLSERGADRAAR"
     gene            816..1352
                     /locus_tag="PLP50_17640"
     CDS             816..1352
                     /locus_tag="PLP50_17640"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPA53419.1"
                     /translation="MNLAAAFSASSSSGAPPRRARATSVALALVAVLLSCRPSAGHEA
                     FTGKVVGVADGDTLVVLDGTTPVRVRLHGIDCPERGQAFGAAAKRLASSLAFGKRVTV
                     QERGKDRYGRLLGEVVLPDGRVLNRELVAAGMAWHYVRYSDDAALAKAERRAREARVG
                     IWSEPDPVAPWSYRAASR"
     gene            complement(1373..1756)
                     /locus_tag="PLP50_17645"
     CDS             complement(1373..1756)
                     /locus_tag="PLP50_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPA53420.1"
                     /translation="MGKVVLDASAILALLNDEPGAAAVAAALEDAVVSAVNLCEVVAK
                     LQEVGMSREEAEEALGGLGLEVQPFGEAAAWAAGTLRGATRRAGLSLGDRACLALARE
                     LGVPAITADAAWLKAATGADVRLVR"
     gene            complement(1749..1997)
                     /locus_tag="PLP50_17650"
     CDS             complement(1749..1997)
                     /locus_tag="PLP50_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPA53421.1"
                     /translation="MNEIAARIDTSGRLVIPARIRKDLGLTAGTAVTLRVSDGELRVV
                     AKAEAIRAAQARVRRYVKPGRSLAGELIAERRAEWRRG"
     gene            complement(2219..2293)
                     /locus_tag="PLP50_17655"
     tRNA            complement(2219..2293)
                     /locus_tag="PLP50_17655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2259..2261),aa:Pro,seq:tgg)
     gene            2349..3104
                     /locus_tag="PLP50_17660"
     CDS             2349..3104
                     /locus_tag="PLP50_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPA53422.1"
                     /translation="MSRTILLLHGLGGSAEDWKEVVAALPKGLLALALDFPGFGKAAR
                     PHDSYDPVALARWVVGELDDRDVETAVVAGHSIGGRVAAELALLAPKRVAALALVSPL
                     GAVSYGFTETLKWKAMSRAALVAAAPETSLRNALGYGFAVDGAGKKGFVARGMKARTG
                     ANAAAALRALERSVDGLLSAAPLRKRLDGTTVPLLVATGAEDPLAPPRDVKAILEARS
                     DARYAELPGVGHYAPLEAPKAVAKLLAELAKKG"
BASE COUNT          411 a         1176 c         1292 g          405 t
ORIGIN      
        1 gggcgaggcc gctgctctcc ttcacctggt cgctcgcgag cgtggcgagg acgcgcgccc
       61 ggccagggct cgccgcctcg gcggccggcg ggggcaggaa gaggagagcg acggccgcgg
      121 cgacggcgtg cgacgcgctc atcgggccgc tcgatcggcc ccgcgctccg agagcggagg
      181 gacggcgacg agcccgaggc cggacgcgtc ctcgacgagg aagtgcccgg agaggcgggc
      241 gacgagcgtc cacgcaccgt cggccttccg gaggaggagg acgtagccgg gaagcgaata
      301 ctccggccag ggcccgccga acacccgttc gcgggagaag aggttctcgc gaaggtcgcg
      361 gagccggagg tcgtcggggc cgtgcgccgc cgggaggtgc caggcgttga agaggacgat
      421 cgcctggagc ggctcggccg cgtcgaggtc gacagcgccg gtccggagcg gaccggaagc
      481 ggcgaggagc ggccaggctt cgcggttcat caaggcgtgg agcgatctga agccgaggcc
      541 gccgacgagg ccgtcttcgg tcgcccgggc ggtggactcc ctctgcgcga gcggcgccgc
      601 cggcgagggc gcgagctcct gcgcggccgt gccgacggaa cctgcagcga cgagaatcgg
      661 gacggcgagg aggaggcttc gtgcgcggcg tgacatgctt ccattctcgg acaggccacc
      721 gttgccggcg cgaacgacgt tcccctctcc cgggcgccgg gcgccggcag tccgcgcttc
      781 tccgattccc ggttccatct cagactgcac gccgcgtgaa cctcgccgct gcgttctccg
      841 catcttcgtc ttccggcgcg ccgcctcgac gcgctcgggc aacgtccgtc gcgctcgccc
      901 tcgtcgccgt cctcctttcc tgccggccgt cggccgggca cgaggcgttc acggggaagg
      961 tcgtcggcgt cgccgacggc gacacgctcg tcgtcctcga cgggacgacg ccggtgcggg
     1021 tgcgtctcca cgggatcgac tgccccgagc gggggcaggc gttcggcgcc gcggcgaagc
     1081 ggctcgcgtc gtcgctcgcg ttcgggaaga gagtgacggt gcaggagcgc gggaaggacc
     1141 gctacgggcg cctcctcggc gaggtcgtcc tccccgacgg ccgggtgctg aaccgcgagc
     1201 tcgtcgccgc cgggatggcg tggcattacg tgcgctactc ggacgacgcg gcgctcgcga
     1261 aggcggagcg gcgggcgcga gaggctcggg tcgggatctg gtcggagccc gacccggtcg
     1321 cgccctggag ctaccgggcc gcgagccgct gaggcgggcg cggggcctcg ggtcagcgga
     1381 cgagccggac gtccgccccg gtcgccgcct tcagccaggc cgcgtcggcc gttatcgcgg
     1441 ggacgcccag ctcccgcgcg agggcgagac aggcccggtc gccgagagag aggcccgcgc
     1501 gccgtgtcgc cccccggagc gtgccggcgg cccaggcggc cgcttcgccg aagggctgca
     1561 cttcgaggcc gagcccgccg agggcctcct cggcctcctc ccgtgacatg ccgacctcct
     1621 ggagcttggc gacgacctcg cagaggttca ccgcgctcac gaccgcgtcc tcgagcgcgg
     1681 cggccacggc cgccgcgccc ggctcgtcgt tcaggagggc gaggatcgcc gaggcgtcga
     1741 gaacgacctt acccacgccg ccactccgcc cgccgctcgg cgatgagctc ccccgcgagc
     1801 gagcgcccgg gtttcacgta acggcggacc ctcgcctgcg ccgcgcgaat cgcctcggcc
     1861 ttcgccacga cgcgcagctc gccgtccgag acccggagcg tgaccgccgt ccccgccgtg
     1921 agccccaggt ccttccggat ccgagccgga atgacgagtc tgcccgaggt atcgatccgt
     1981 gccgcaatct cgttcacctg ccacctcgag ccgagtctac cgccgtgcgc gaggagaggg
     2041 cgcggtgtcg gtcgcagggc cggggcgggc cgggcgggcg ggggcgcccg tcggcgtgcc
     2101 ccgcggagtc tggcgtccgg acctacggat ctgagacgat cccgcagccg tgagaaacgc
     2161 cgagggtgtc gcgccgggga ggggcgcgtg agcgatcgcg cgttcggaag ggaaggagtg
     2221 gtcggggcgg gcggatttga accgccgacc tgctgctccc aaagcagctg cgctgccact
     2281 gcgctacgcc ccggcgcgcc ggattctcgg gccacggcgg agggatgtcc acccgaggga
     2341 ggcgcggagt gagccggacg atccttctgc tgcacgggct gggcggctcg gcggaggact
     2401 ggaaggaggt cgtcgcggcg ctcccgaagg ggctcttggc gctcgctctc gacttccccg
     2461 gcttcgggaa ggcggcgcgg ccgcacgaca gctacgaccc ggtcgcgctc gcgcggtggg
     2521 tggtcggcga gctggacgac cgcgacgtgg agacggccgt cgtcgcgggg cattcgatcg
     2581 gggggcgcgt cgccgccgag ctggcgctcc tggcgccgaa gcgggtcgcg gcgctcgcgc
     2641 tcgtctcgcc gctcggggcc gtctcctacg gcttcaccga gacgctgaag tggaaggcga
     2701 tgtcgcgcgc cgcgctcgtc gccgcggcgc cggagacgtc gctccggaac gcgctcggct
     2761 acggcttcgc ggtggacggc gccgggaaga aggggttcgt ggcgcgcggg atgaaggcgc
     2821 ggaccggggc gaacgccgcc gcggcgctcc gggcgctcga gcgctcggtg gacgggctcc
     2881 tctcggccgc gccgctccgc aagcgcctcg acgggacgac ggtgccgctc ctcgtggcga
     2941 ccggcgcgga ggacccgctc gcgccgccgc gcgacgtgaa ggcgatcctc gaagcgcgct
     3001 ccgacgcgcg gtacgcggag cttcccggcg tcggccacta cgcgccgctc gaggcgccga
     3061 aggcggtggc gaagctcctc gcggagctcg cgaagaaagg ctgaaggccg cgcctcaggg
     3121 cgcggcgagg acgctcgcga gccaggcgcg gaagcgcgcg agggtcgcgg gcgagggggc
     3181 ctcgtgaccg gcctcgtcct ccacggcgag ctccacctcc gcccccgcag cgcggagcgc
     3241 cgcgacggcc tgctccgtca gctcgaaggg agtgttgcgg tcct
//
LOCUS       DAOONL010000250         3255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_35839_length_3255_cov_15.285000, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000250 DAOONL010000000
VERSION     DAOONL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35839_length_3255_cov_15.285000"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLP50_17665"
     CDS             <1..289
                     /locus_tag="PLP50_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HPA53423.1"
                     /translation="KDGVGGNAWLRVVLGEGRSRQVRRMLESVGHKVSKLRRVAIGPI
                     RDAKIPVGGFRDLSPAEVAALRAVQPAARPTPRRKPAVPNPRAPRRRRKEE"
     gene            289..1431
                     /gene="hisC"
                     /locus_tag="PLP50_17670"
     CDS             289..1431
                     /gene="hisC"
                     /locus_tag="PLP50_17670"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPA53424.1"
                     /translation="MKPSPRPSIAKLAPYVPGRPIEEVEREYGITGAVKLASNENPLG
                     PSPKGVAAAVAAASGVNFYPDGSATYLRRALSSRFGFPSEQVVIGSGSSELIDLCMRA
                     FVDPGDEVVVPQGIFRMFPVAVGRAGGTIVEVPTKPGHVPDLDALKRRIGPATKVVAI
                     ANPNNPTGAYVPRAELERFFEGLPDHVVAVVDEAYHEFAREVADYPNALDELRKGRNV
                     LVLRTFSKAYGLAGFRIGYGFTTPEIAAAINKVREPFNTTVVAQVAAIAALDDEEHCE
                     RTVSLVRTERAFLAAELARRQAVVNPSLGNFLLVELPIPFGPLEPEFARRGVILRPMG
                     GWGFPLAFRVSVGTHDENVRFLAAFDDLLANGKLGLPAVAVPVGAG"
     gene            1428..2102
                     /gene="cmk"
                     /locus_tag="PLP50_17675"
     CDS             1428..2102
                     /gene="cmk"
                     /locus_tag="PLP50_17675"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPA53425.1"
                     /translation="MIVVAIDGPSGVGKSTVGRALAASLGVPYLDTGAMYRAVGLLAR
                     RKGLPLPLTGADEVGELAERTEILVTGSGGDVKTFLDGEDVSAEIREPEISRYASAVA
                     AIPRVRRRLAARQREMALAGGGVLEGRDIGTRVVPEATAKFFLTARPEVRARRRYEEL
                     LRKGTPQELAAVRAEMEARDAADTSRADSPLSCDDTYVVVDTSDLAAGEVVERLRQLV
                     LGSRTG"
     gene            2195..>3255
                     /locus_tag="PLP50_17680"
     CDS             2195..>3255
                     /locus_tag="PLP50_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPA53426.1"
                     /translation="MPSEDQKITPIRKPEEAGDANGEFARLLAESERSLAEGELIRGR
                     VIQVTNDEILVDIGYKSEGMIPRSEFNHSPDRLPQAGQEIDAIVDRREDASGYVVLSH
                     EKARRIRAWDAIEVAFKDNVAVKGKVLERVKGGLSVDIGVRAFLPGSLADIRPLKNLD
                     VLRGRELEFRIVSFDKKRGNIVLSRKALMEEVAEAKKAEAAQALADGRTLHGVVKNLT
                     EYGAFVDLGGIDGLLHVTDISWGRLGHPSEALQVGQEVEVKVLKFDADSGRISLGMKQ
                     LKPDPWQDLPAHYPVGARVRGKVVSLTDYGAFIELEEGVEGLIHVSEMSWTKKVKNPA
                     KLLSVGDTVEAVIADVNPET"
BASE COUNT          493 a         1139 c         1147 g          476 t
ORIGIN      
        1 gaaggacggc gtcggcggga acgcctggct ccgcgtcgtc ctcggcgagg ggcgcagccg
       61 ccaggtccgc cggatgctgg agtccgtcgg acacaaagtc tcgaagctcc gtcgcgtcgc
      121 gatcggcccg atccgcgacg cgaagatccc cgtcggcggc ttccgcgacc tcagtccggc
      181 ggaggtcgcg gcgcttcgcg cggtgcagcc cgccgcgcga ccgacccctc ggcgaaagcc
      241 tgccgtcccc aaccccaggg cgcctcgccg gcgccgcaag gaggagtgat gaagccctct
      301 ccgagaccct cgatcgcgaa gctcgccccg tacgtcccgg gacgccccat cgaagaggtg
      361 gagcgcgagt acgggatcac cggagccgtg aagctcgcct cgaacgagaa cccgctcggg
      421 ccctccccga agggcgtcgc cgccgcagtt gccgcagcgt ccggggtgaa cttctacccg
      481 gacggctccg ccacgtacct tcggagggcg ctctcgtcgc gcttcggctt cccgagcgag
      541 caagtcgtca tcgggtcggg ctcctcggag ctgatcgacc tctgcatgcg tgcgttcgtc
      601 gacccgggcg acgaggtcgt cgtgccgcag gggatcttcc ggatgttccc ggtcgccgtc
      661 gggcgggccg gcggcacgat cgtggaggtc ccgacgaagc ccggccacgt cccggacctc
      721 gacgcgctga agaggcggat cgggcccgcg acgaaggttg tcgcgatcgc gaacccgaac
      781 aacccgacgg gagcctacgt cccgagggcc gagctcgagc ggttcttcga gggcctgccg
      841 gaccacgtcg tggccgtcgt cgacgaggcc taccacgagt tcgcgcggga ggtcgccgac
      901 tatccgaacg ccctcgacga gctgcggaag ggacggaacg tcctggttct ccggacgttc
      961 tccaaggcct acggtctcgc ggggttccgg atcggctacg gcttcacgac gcccgagatc
     1021 gccgccgcga tcaacaaggt gcgggagccg ttcaacacga cggtcgtggc ccaggtcgcc
     1081 gccatcgccg cgctggacga cgaggagcac tgcgagcgga ccgtgtcgct cgtccggacg
     1141 gagcgggctt tcctcgcggc cgagctcgcc cggcggcagg ccgtggtcaa cccgtcgctc
     1201 ggcaacttcc tcctcgtcga gcttccgatt ccgttcggcc ctctcgagcc ggagttcgct
     1261 cgccggggcg tgatcctgcg tccgatgggg ggatggggct ttcctctggc gttccgcgtg
     1321 tcggtcggaa cgcacgacga gaacgtccgg ttcctggccg ccttcgacga cctcctcgcg
     1381 aacggtaagc tcgggcttcc ggcggtcgcg gtccccgtcg gcgccggatg atcgtcgtcg
     1441 cgatcgacgg tccctcgggc gtcggcaagt cgacggtggg gagggccctg gccgcgagcc
     1501 tcggcgttcc ctacctcgac accggtgcca tgtatcgcgc cgtcggcctc ctggcgcgcc
     1561 ggaaggggct gccccttccg ctgaccgggg cggacgaggt gggcgaactc gcggagcgga
     1621 ctgagatcct cgtgaccggc tccgggggcg acgtgaagac gttcctcgac ggcgaggacg
     1681 tctccgccga gatccgcgag ccggagatct cgcgctacgc ctccgcggtc gcggcgatcc
     1741 cgcgggtccg aaggcgcctg gcggcccgcc agcgcgagat ggccctcgcg ggcggcggcg
     1801 tcctcgaggg acgggacatc ggaacgaggg tcgtcccgga ggcgaccgcg aagttcttcc
     1861 tcacggcgcg tcccgaggtc cgggcccggc ggcgctacga agagctgctc cggaagggga
     1921 ctccccagga gctggccgcc gtgcgggccg agatggaggc gcgggacgcg gccgacacgt
     1981 cccgtgccga ctcgcccctc tcctgcgacg acacctacgt cgtcgtcgac acctccgacc
     2041 tcgcggccgg cgaggtggtc gagaggctcc ggcagctcgt cctcggctcc cgaaccggtt
     2101 gacctgtcgg aggctcccgt ctaacattcc cggttcgggc cgctaactca agacccttga
     2161 gccgtctccg cgagccctcg agaaggtgga attcatgccg tcggaggacc agaagatcac
     2221 gcctatccgg aagccggagg aggctggcga cgcgaacgga gagttcgcgc gcctgctcgc
     2281 agagagcgag cgttccctcg ccgagggaga gctgatccgc gggcgtgtca tccaggtcac
     2341 gaacgacgag atcctcgtcg acatcggcta caagtcggag gggatgatcc cgcggtcgga
     2401 gttcaaccac tcgccggatc gcctcccgca ggccggacag gagatcgacg cgatcgtcga
     2461 ccgtcgcgag gacgcttcgg gctacgtcgt cctctctcac gagaaggcgc gccgcatccg
     2521 cgcgtgggac gcgatcgaag tggccttcaa ggacaacgtg gcggtcaagg ggaaggtcct
     2581 cgagcgcgtg aagggcgggc tgtccgtcga catcggcgtg cgggctttcc tgccggggtc
     2641 gctcgcggac atccgcccgc tgaagaacct cgacgtcctg cgcggccgcg agctcgagtt
     2701 ccgcatcgtc agcttcgaca agaagcgcgg gaacatcgtg ctctcgcgca aggcgctcat
     2761 ggaagaggtc gccgaggcga agaaggccga ggccgcccag gccctcgcgg acggccgcac
     2821 gctccacggc gtcgtcaaga acctgaccga gtacggcgcg ttcgtcgacc tcggcgggat
     2881 cgacggcctc cttcacgtga ccgacatctc gtggggccgg ctcgggcacc cgtccgaggc
     2941 cctccaggtc ggccaggagg tcgaggtcaa ggtcctcaag ttcgacgcgg attcgggccg
     3001 gatctccctc ggcatgaagc agctcaagcc cgacccgtgg caggacctcc cggcccacta
     3061 tccggtcggg gcccgcgtcc gcggcaaggt cgtctcgctc accgactacg gcgcgttcat
     3121 cgagctcgaa gagggagtcg agggcctcat ccacgtcagc gagatgtcgt ggacgaagaa
     3181 ggtcaagaac cccgcgaagc tcctctcggt gggggacacc gtcgaggcgg tcatcgccga
     3241 cgtgaacccc gagac
//
LOCUS       DAOONL010000251         3244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_35999_length_3244_cov_13.900282, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000251 DAOONL010000000
VERSION     DAOONL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35999_length_3244_cov_13.900282"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLP50_17685"
     CDS             <1..603
                     /locus_tag="PLP50_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPA53427.1"
                     /translation="LFDAVVSFLVTLFVLFFLFRDGRAMVRAVLDLVPLEETRRVAVF
                     ASLRGMLQSIFRGSLLCALIQGATGGIGWAIAGLPSPVLAGAAMAVLSLLPIGGTALV
                     WAPGAVACWLQGRHGMAIFLAVWGLVVVSTLADNVLKPILIGGGTQLSTLVVFLGVFG
                     GLAAFGILGLFIGPMVLAFALTLVGILREAVHPKAEAGAA"
     gene            complement(611..1753)
                     /locus_tag="PLP50_17690"
     CDS             complement(611..1753)
                     /locus_tag="PLP50_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53428.1"
                     /translation="MRRDVLLLLSAAALLGSAACDSSRPSATEVTTAATPAAPDAPAR
                     RAGPWEGVLSPLKWPGERHLANVRQLTFGGENAEAYWSADGKKLVFQATRPPFRCDQI
                     FVVDADDPGEPRLVSTGKGRTTCAYWFPDGERILFSSTHLGGGPLCPPPPDMSSGYVW
                     SVNPDYEIFSARPDGSGLRRLTASIGYDAEATISTDGRRIVFTSARDGDLELYSMNAD
                     GNDVRRLTRTPGYDGGAFFSDDGEWIVYRASRPATPQLVEEFLSLLRRHLVRPSRLEI
                     RMMRADGSDDRQVTSHGVASFAPFFFRGGHDRIVFVSNLADPGGRDFDIWAVRSDGTD
                     LERITYNPTFDGFPMFSPDGRRLAFCSNRHNGRPGETNVFVADWVP"
     assembly_gap    1794..1893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1894..2677
                     /locus_tag="PLP50_17695"
     CDS             <1894..2677
                     /locus_tag="PLP50_17695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA53429.1"
                     /translation="ASEDLRAARLEWTTLRTELRSGTPLANLDEEAAPGPETRSASDP
                     ISAGLYADFERAFRGAESEIRRRQDGDVALFAGSRLPIADLGCGRGEFLEALRARGLQ
                     GLGCDTNPVMVARCREKGLAVDEADLFAWLRARAVDSLGGVTAYQVVEHLPADALLPF
                     VELAVSRLAPGGRLLLETVNAESVYAMKWFWMDLTHVRPVPAPSLGRLMEACGLRNVT
                     IAHRSPVPREESGGGVEPADPLVAALARLVFAPQDVAVTGVK"
     gene            2678..>3244
                     /locus_tag="PLP50_17700"
     CDS             2678..>3244
                     /locus_tag="PLP50_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPA53430.1"
                     /translation="MANVLVCTAQVPFASGGAERHAAGLVRELRARGHRVDLVQLPFK
                     WYPREEILLSAAAWRLVDVTEADGTKVDLVVPMKFPSYLVRHPNKVVWLIHQFRQAYD
                     RFGTEQSDFGPGPEDTRWRELIAEADTTGLGEARKVYTNARNTADRLMRFNGIEAEAL
                     YHPPPLAGRYRDDGPGRWALVVGRLDPWK"
BASE COUNT          404 a         1101 c         1165 g          474 t
ORIGIN      
        1 ctcttcgacg ccgtcgtctc gttcctcgtc acgctcttcg tcctcttctt cctctttcgc
       61 gacgggcgcg cgatggtccg cgcggtcctc gacctcgtgc cgctcgagga gacccgccgg
      121 gtcgccgtct tcgcgtcgct ccgcgggatg ctccagtcga tcttccgggg ctcgctcctg
      181 tgcgcgctca tccagggagc gacgggcggc atcggctggg cgatcgcggg cctgccgtct
      241 ccggtcctcg ccggcgcggc gatggcggtc ctctcgctcc tcccgatcgg aggcacggcg
      301 ctcgtctggg cgcccggggc cgtcgcctgc tggctccagg ggcggcacgg tatggcgatc
      361 ttcctcgcgg tctggggcct cgtcgtcgtc tcgacgctcg ccgacaacgt cctgaagccg
      421 atcctgatcg gcggcgggac gcagctctcg acgctcgtcg tcttcctcgg cgtcttcggc
      481 ggtctcgccg cgttcgggat cctcggcctc ttcatcgggc cgatggtcct cgccttcgcc
      541 ctgacactcg tcgggatcct tcgcgaggcg gtccacccga aggccgaggc cggggcggcc
      601 tgacgcgacg ctacgggacc cagtcggcga cgaagacgtt cgtctccccg ggccttccgt
      661 tgtggcggtt cgagcagaat gcgaggcgcc ggccgtcggg agagaacatc gggaagccgt
      721 cgaacgtcgg gttgtaggtg atcctctcga ggtcggtgcc gtcggagcgg acggcccaga
      781 tgtcgaagtc gcggccgccg gggtcggcga ggttcgacac gaagacgatc cgatcgtgcc
      841 ctccccggaa gaagaacggc gcgaagctcg cgacgccgtg gctcgtcacc tgccggtcgt
      901 ccgagccgtc ggcgcgcatc atccggatct cgaggcggct cggccggacg aggtgccggc
      961 gaagcagcga gaggaactcc tcgacgagct gcggcgtcgc cgggcgggaa gcgcggtaga
     1021 cgatccactc gccgtcgtcg ctgaagaacg cccctccgtc gtacccgggc gtccgggtca
     1081 gccggcggac gtcgttcccg tcggcgttca tggagtagag ctcgaggtcc ccgtcccgcg
     1141 ccgacgtgaa gacgatcctc cggccgtcgg tcgagatcgt cgcctcggcg tcgtacccga
     1201 tgctggccgt gaggcgccgg aggcccgagc cgtcgggacg ggcgctgaag atctcgtagt
     1261 ccggattcac gctccagacg tatccggagg acatgtcggg cggagggggg cagagcggcc
     1321 cgccgccgag gtgggtcgag gagaagagaa tccgctcccc gtccgggaac cagtaggcgc
     1381 aggttgtccg ccccttgccg gtcgagacga ggcggggctc gcccgggtcg tccgcgtcga
     1441 ccacgaagat ctggtcgcag cggaaggggg ggcgcgtggc ctggaagacg agcttcttcc
     1501 cgtcggccga ccagtacgcc tcggcgttct caccgccgaa cgtgagctgg cggacgttcg
     1561 cgaggtgccg ttcgcccggc cacttgaggg gcgagaggac gccctcccag gggcccgccc
     1621 ggcgggccgg cgcgtccggc gcggcgggcg ttgcggccgt ggtcacctcg gtggccgacg
     1681 gccgggacga gtcgcacgcg gcggagccga ggagagctgc ggcggagagg aggaggagga
     1741 cgtcgcggcg catgggtggg ccgtaatata aggcggatga cgaacgaccg acgnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcctcg gaggacctgc gggcggcgcg
     1921 cctcgagtgg acgacgctcc ggaccgagct ccggagcggc acccctctcg cgaacctgga
     1981 cgaagaagcg gcgcccggac cggagacgcg ctccgcgtcc gacccgatct ccgcgggcct
     2041 ctacgcggac ttcgagaggg cgttccgggg cgcggagtcg gagatccgtc gccggcagga
     2101 cggggacgtc gccctcttcg ccgggagccg gctcccgatc gcggatctcg gctgcggacg
     2161 gggggagttc ctcgaggcgc tccgcgcgcg cgggctgcaa ggtctcggct gcgacacgaa
     2221 ccccgtgatg gtcgcgcgct gccgcgagaa ggggctcgcg gtcgacgagg ccgatctctt
     2281 cgcctggctc cgcgcgaggg cggtcgattc tctcggtggg gtgacggcgt accaggtcgt
     2341 cgagcacctc ccggccgacg cgctcctgcc gttcgtcgag ctcgccgtct cgcgcctcgc
     2401 gccggggggg cggctcctgc tggagaccgt gaacgccgag tcggtctacg cgatgaagtg
     2461 gttctggatg gacctcacgc acgtccgacc cgtcccggcg ccgtcgcttg gacgcctgat
     2521 ggaggcctgc ggcctgcgga acgtcacgat cgcccatcgc tcgccggtcc cccgggagga
     2581 gtcgggcggc ggcgtggagc cggccgaccc cctcgtcgcc gccctggcgc ggctcgtctt
     2641 cgcgccgcag gacgtcgcgg tgacgggggt gaagtaggtg gcgaacgtcc tcgtctgcac
     2701 ggcgcaggtg ccgttcgcct cggggggcgc cgaacgccac gccgccggcc tcgtccgcga
     2761 gctccgcgcg cgcgggcatc gggtcgacct cgtccagctc ccgttcaagt ggtacccgag
     2821 ggaggagatc ctcctctccg ccgcggcgtg gcgcctcgtc gacgtcacgg aggccgacgg
     2881 gacgaaggtg gacctcgtcg tcccgatgaa gttcccgtcg tacctcgtgc gtcacccgaa
     2941 caaggtcgtc tggctgatcc accagttccg ccaggcctac gaccgcttcg ggacggagca
     3001 gagcgacttc ggcccgggac cggaagacac gcgatggcgg gagctgatcg ccgaggccga
     3061 tacgacgggc ctcggcgagg ctcgcaaggt ctacacgaac gcgcggaaca ccgccgaccg
     3121 cctcatgcgc ttcaacggca tcgaggccga agcgctctac cacccgccgc cgctcgcggg
     3181 gcgctatcgc gacgacggcc cgggccgctg ggctctcgtc gtcggccgcc tcgacccgtg
     3241 gaag
//
LOCUS       DAOONL010000252         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_36180_length_3232_cov_9.499528, whole genome shotgun sequence.
ACCESSION   DAOONL010000252 DAOONL010000000
VERSION     DAOONL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36180_length_3232_cov_9.499528"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..213)
                     /locus_tag="PLP50_17705"
     CDS             complement(22..213)
                     /locus_tag="PLP50_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53431.1"
                     /translation="MAETDTIARLTRERDEAREACRLIDLKIGHFGSAPEPPQDMPML
                     WLLWYAGRTARAALREEGK"
     gene            complement(437..1696)
                     /locus_tag="PLP50_17710"
     CDS             complement(437..1696)
                     /locus_tag="PLP50_17710"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA53432.1"
                     /translation="MKVQKIRIENILGLETLEFEPGRVTIVSGRNGVGKSSVLESLKY
                     ALTGGSDATIKRKGADKGEVVIVLDEATVTRDTEKAAVVSVTGVGPIRRPAEFLKSRI
                     DSLSFNPVQFLLAPKDKRTEWFLEMVPVEVGDDELRAAGVETLPPPARNGLDRIAAAT
                     KLVYDARTGVNRALKEKRAAVAQLAEGLSEVADVDLAALRAEKDRLSTALAADLSGVK
                     DRAAGLLAKLREDAQARIDEIKATLATEERLVQEELVRETDRLRGIAQPEIEQAAAAL
                     AVAESQAKAAAQMEKTRELIARMTTEAAALEEESARRSATLDNLDALKRRALDRLPIQ
                     GVEIADGEILVNGIPFDRVNTAARVQFVLNVAMLRKGTLPLVLLDGLECLDPETFEAF
                     VNAASHLDAQFICTRVTEGPLEVRVAA"
     gene            complement(1693..2499)
                     /locus_tag="PLP50_17715"
     CDS             complement(1693..2499)
                     /locus_tag="PLP50_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPA53433.1"
                     /translation="MSSIRRIDARGRRTYDIPGVGELVSVTTYLQAVPKPFLVSWAAR
                     RERELVVNAAVRLGTVTRATLLSQLPATFAHEIELDVAGELGSEAHALIEWWLRGEIG
                     PRPPARPGAEMAFRAAQKWAASVQLEPIFCEQTVWSEEWGFAGTMDLFGRVNGKLALI
                     DWKTSKRFYPEHGMQSAAYITALDEMGHAKSDEVLGIVVRLPKETDGPAEAHILEPSH
                     AERFRRFALAMETWRWMREVEGAYVPKAALPIDRPEQITASNAFSFTERV"
     gene            complement(2504..3025)
                     /locus_tag="PLP50_17720"
     CDS             complement(2504..3025)
                     /locus_tag="PLP50_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53434.1"
                     /translation="MAIVAKSADNGTFVNAPQGSHQAVCCDVIDVGLVDVVQPDKSVK
                     KVHKIDIVWQLPIVNEESGKRFTVRKRFTLSLSEKANLRKDLEAWRGKPFPKEIVETG
                     FDVENLLGVNALLNVQHEASREGRVYANVKAIMALPQDMTPMVAVDYVRKCDRDQAPP
                     PVSTPAVSASSTF"
BASE COUNT          439 a         1144 c         1097 g          552 t
ORIGIN      
        1 gggtgatgac gtgtggcagc ttcacttccc ctcctcccgg agcgcggcgc gggccgtccg
       61 ccccgcatac cagagcagcc acagcatcgg catgtcctgc ggaggctcgg gcgctgagcc
      121 gaaatggccg atcttcaggt cgatcaatcg gcacgcctcc ctcgcctcgt cccgctcgcg
      181 cgtcaggcgc gcgatggtgt cggtctcagc catggggctc tctcctgttg ggtaatgccc
      241 gcccgccggg tctccccgga ttgcgccgaa tcagtagatg cgctcgcagc gcgacgcatt
      301 cgggcgggtt caagaaaacg cgcgccgccg gtagcccgtc cgctcggggc gattccacga
      361 tgcccctccc accctcgctg ggccggtgct cccggcctgt ccgcgtggtc ccgtgcgtcg
      421 gcggcgcgcg actgattcac gccgcgaccc tcacctcgag cggtccctcc gtcacccgcg
      481 tgcagatgaa ctgcgcgtcg agatgggagg cggcgttgac gaacgcctcg aacgtctccg
      541 ggtcgaggca ctccagcccg tcgaggagta cgagcgggag cgtccccttc cggagcatcg
      601 ccacgttcag cacgaactgc acccgggcgg ccgtgttcac tcggtcgaac gggatgccgt
      661 tgacgaggat ctcgccgtcg gcgatttcga cgccctggat cggcaggcgg tcgagggccc
      721 ggcgcttgag cgcgtcgagg ttgtcgagcg tggccgacct gcgagccgac tcctcctcga
      781 gcgccgcggc ctcggtcgtc atcctggcga taagctcgcg cgtcttctcc atctgcgcgg
      841 cggccttggc ctgcgactcg gccacggcga gggcggcggc ggcctgctcg atctcgggct
      901 gagcgatccc gcgaagccga tcggtctccc ggaccagctc ctcctggacg agacgctcct
      961 ccgtggcgag ggtcgccttg atctcgtcga tgcgggcctg cgcgtcctcg cggagcttcg
     1021 cgagcagccc agcggcccgg tccttcacgc cgctcagatc cgccgccagc gccgtcgaga
     1081 gtcggtcctt ctcggcacga agcgccgcca ggtccacgtc cgccacctcg gacagcccct
     1141 cggcgagctg cgccaccgcg gcgcgcttct ccttcagcgc acggttgacg ccggtccgcg
     1201 cgtcgtagac gagcttcgtc gcggcggcga tccggtcgag cccgttgcgg gccggcggcg
     1261 gaagcgtctc cacgccggcc gcccgtagct cgtcgtctcc gacctcgacc gggaccatct
     1321 cgaggaacca ttccgtgcgc ttgtccttcg gcgcgaggag gaactggacg ggattgaacg
     1381 agagcgagtc gatgcggctc ttcaggaact cggccggccg ccggatcgga ccgacccccg
     1441 tgacgctcac gacggcggcc ttctccgtgt cgcgcgtgac ggtcgcctcg tcgaggacga
     1501 tgacgacctc gcccttgtcg gcccccttgc gcttgatcgt cgcgtcgctg ccgccggtca
     1561 gggcgtactt cagcgactcg aggacggacg acttgccgac cccgttgcgg ccgctgacga
     1621 tcgtcacgcg gcccggctcg aactcgagcg tctcgaggcc gaggatgttc tcgatgcgga
     1681 tcttctgtac cttcataccc tctccgtgaa gctgaaagcg ttgcttgctg tgatctgctc
     1741 cggcctgtcg atcgggagcg cggccttcgg cacgtaggcg ccctctacct cgcgcatcca
     1801 ccgccaggtt tccattgcca gggcgaaccg gcggaagcgc tcggcgtgcg acggctcgag
     1861 gatgtgcgcc tcggccgggc cgtcggtctc cttcggcagc cggacgacga tgccgagaac
     1921 ctcgtcgctc ttcgcgtggc ccatctcgtc aagggccgtg atgtacgccg cactctgcat
     1981 cccgtgctcc gggtagaagc gcttggaggt cttccagtcg atgagagcga gcttcccgtt
     2041 cacgcggccg aacaggtcca tcgtcccggc gaatccccac tcctccgacc agaccgtctg
     2101 ctcgcagaag atcggctcga gctggaccga ggcggcccac ttctgcgcgg cgcggaaagc
     2161 catctcggcc ccggggcgcg cgggaggacg gggaccgatc tccccgcgga gccaccactc
     2221 gatcagcgcg tgcgcctccg agccgagctc cccggccacg tccagctcga tctcgtgcgc
     2281 gaacgtggcg gggagctggc tgagcagcgt cgcgcgggtg accgtcccga gacgcaccgc
     2341 cgcattgacg accagctcgc gctcccgacg ggccgcccag ctcacgagga acggcttcgg
     2401 cacggcctgg aggtacgtcg tcacgctgac cagctccccg acgccgggga tgtcgtaggt
     2461 gcgccggcca cgggcgtcga tgcggcggat gctgctcatc gggtcagaag gtggacgacg
     2521 ccgagacggc gggcgtgctg acgggcgggg gtgcctggtc ccgatcgcac ttccggacgt
     2581 agtcgaccgc gaccatcggc gtcatgtcct gcgggagcgc catgatcgcc ttgacgttcg
     2641 cgtagacgcg cccctcgcgg gaggcctcgt gctgcacgtt gagcagggcg ttcacgccga
     2701 ggaggttctc gacgtcgaag ccggtctcga cgatctcctt cgggaacggc ttgccgcgcc
     2761 acgcctcgag gtccttccgg aggttcgcct tctcggagag gctgagcgtg aagcgcttgc
     2821 ggacggtgaa gcgcttcccc gactcctcgt tgacgatcgg gagctgccag acgatgtcga
     2881 tcttgtgcac cttcttcacg ctcttgtcgg gctgcaccac gtcgacgagg ccgacgtcga
     2941 tgacgtcgca gcacacggcc tggtggctgc cctgcggggc gttgacgaag gttccgttgt
     3001 cggcggactt ggcgacgata gccattgatg gctccttctg ccccttctgg ggcggttaga
     3061 cggccttccg gccgttgctt gtggagttgc agtagtcgca tcgcccaccc cgctcggccc
     3121 gctcccacgc cgcagcggcg caggagagga ggaagaagcc ggcagtcgtg acgagcagga
     3181 gcccgaaggc gtccgaggcg agaaggtcgg tgccgatctt gtgtagcgcg tc
//
LOCUS       DAOONL010000253         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_36296_length_3224_cov_11.881351, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000253 DAOONL010000000
VERSION     DAOONL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36296_length_3224_cov_11.881351"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PLP50_17725"
     CDS             complement(<1..981)
                     /locus_tag="PLP50_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53435.1"
                     /translation="MIVETVDRAEVRRTLGLLFYNSHLFEIRVLDASNVRCNGRPEGR
                     NATFFGYFDSTETAANVVHAALSGADWTGAYVTMNPVRNDLLARAANRLRQAGKKGGS
                     ASDVDVPRRRHIVFDFDPVRPAGISSTDDEKAAAQVLAHRVRLYLSGIGWPEPIVADS
                     GNGWHLLYAVDLPGNDGGLVKACLEALAARFDSDETKVDTTVFNPARITKLYGTLARK
                     GDHTPERPHRMSRIIEVPDRIENVPEERLRDLAAQAPQAGEQKKPGIVAHSTPGTFDL
                     ESWIEQHIPHAGPREPWQGGSRWIVNPCVFDENHTGTSAAIVRNAAGVIAY"
     gene            complement(978..1205)
                     /locus_tag="PLP50_17730"
     CDS             complement(978..1205)
                     /locus_tag="PLP50_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53436.1"
                     /translation="MSTCPCRRAAARTRRRDVVRDLARSHRYSYLMFSLYLAQDLMQA
                     SRDAEPDEQHRLRELARTIRAGVRFAQGGAE"
     gene            complement(1417..1623)
                     /locus_tag="PLP50_17735"
     CDS             complement(1417..1623)
                     /locus_tag="PLP50_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53437.1"
                     /translation="MTTKPDRSRPKPAGRPLAYRLVDLPPLVGISQSGLRNMIAAGTL
                     RAVRRGRVVLVRAAEVDRLVGGEG"
     gene            complement(2397..2972)
                     /locus_tag="PLP50_17740"
     CDS             complement(2397..2972)
                     /locus_tag="PLP50_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53438.1"
                     /translation="MEQAVEKITNLSRSVAEQHATMEAAFDEERAAEEELLEKLVDAV
                     RPALRALSSRLKASETTWWPTSTETSTEKTYHPERGVRLDGNGPERDHPRANDGSIGG
                     TDLVLLDDGTFARLDWTGSWTRWQGRTSEETSTLTRIALRDVVETWDVDEIAATLSER
                     LEAQLAGRATKTTASARQRTEKVRAIAALMK"
BASE COUNT          429 a         1018 c         1237 g          540 t
ORIGIN      
        1 gtaggcgatg acgcccgcgg cgttgcggac gatggcggcg gaggtgccgg tgtggttctc
       61 gtcgaagacg cacgggttga cgatccagcg cgatccgccc tgccagggct cgcgcgggcc
      121 ggcgtggggg atgtgctgct cgatccagct ctcgaggtcg aacgtgccgg gggtcgagtg
      181 cgcgacgatg ccgggcttct tctgctcgcc ggcctgcggc gcctgcgcgg ccaagtcgcg
      241 gagtcgctcc tcgggaacgt tctcgatccg gtcggggacc tcgatgatgc ggctcatcct
      301 gtggggccgc tccggggtgt ggtcgccctt tcgagcgagc gtcccgtaaa gcttcgtgat
      361 ccgtgccggg ttgaagacgg tcgtgtcgac cttcgtctcg tcggagtcga accgagccgc
      421 gagggcttcc aggcacgcct tgacgagtcc gccgtcattg ccgggcaggt cgacggcata
      481 gaggaggtgc cagccgttgc cggagtcggc gacgatgggt tccggccagc cgatcccgga
      541 caggtagagg cggacgcggt gcgcgaggac ctgcgcggcg gccttctcgt cgtcggtcga
      601 cgagataccg gcggggcgga cgggatcgaa gtcgaagacg atgtgccggc gacgagggac
      661 gtcgacatcg gaggccgacc cgcccttctt cccggcctgc cggaggcggt tcgccgctcg
      721 cgcgaggagg tcgttgcgga cggggttcat ggtcacgtag gcgcccgtcc agtcggctcc
      781 ggagagggcg gcgtggacga cgttggcggc cgtctcggtc gagtcgaagt agccgaagaa
      841 cgtcgcgttc cgcccttcgg gccggccgtt gcatcggacg ttggaggcgt cgaggacgcg
      901 gatctcgaag aggtgggagt tgtagaagag gagcccgagc gtgcgccgga cctcggcgcg
      961 gtcgaccgtc tcgacgatca ttccgcccct ccctgcgcga acctgacgcc ggcgcggatg
     1021 gtccgggcga gctcgcggag ccggtgctgc tcgtccggct cggcgtcgcg gctggcctgc
     1081 atgaggtcct gggcgaggta gaggctgaac atgaggtagc tgtagcggtg gctccgcgcg
     1141 aggtcgcgga cgacgtcccg gcgacgcgtg cgggcggcgg cgcggcggca ggggcaggta
     1201 ctcatgccgc gctccgtctg ccggccggcc aggggaggcc gacggcgtag gcgagaccct
     1261 cgggccagcg gtggaagcgc cggaagttga tgacggggaa ggggcggcgg cggtaggctg
     1321 aaaggcgcgt agactctggg gagctgatct ctttgtcttg cgccgcccgg cttcctcgcg
     1381 ccagcgggga gccgggcacc ttcgtcttgg ggcgtctcat ccttcgcccc cgacgagtcg
     1441 gtcgacctca gccgcccgga cgaggacgac ccttccccgg cggacggcgc ggagggtccc
     1501 cgcggcgatc atgttccgga gtccgctctg gctgatcccg acgaggggcg ggaggtctac
     1561 cagacggtaa gcgagggggc gaccggcggg cttgggtcgg ctgcggtcgg gcttcgtcgt
     1621 catgtgtgtc tcgctttctc ccggcgcgtt ctgcgctagg tggacgcgaa tctagggccg
     1681 tgttctgaga tgagcgagtt gggggggaat tcgctaacgc ggcgagcagt tcggggctcg
     1741 catttttgcg tgcctgcgag atgaggacgc ggatcgcgcc gggggacgcg acgccaaacc
     1801 tatccatgat ctgctcgtcg gtgtacccgt aagcgcgagc caacgcgacc gacccgacga
     1861 gacgggagcg tggctttccc cggcgtccgg gagggaccga atccgccagg agttcccgga
     1921 gccgagcgcg agtcagtccg ccgccgggct ctctgcctgc gcgcgacctg gcgacgtcac
     1981 gccagaccat cgggaggagg agcgggttga cgtcgggcgg ggcgccgacg ccgtacattg
     2041 cctccggcat cggcgcgggg gtcggtctcg ggctcccgtc ggcggggtgg aggctgtcgt
     2101 gccattcggt cgcacggacg gcagcggcaa ggaggtctag cgccggcgcc gcaatcccct
     2161 cccagcagag ccggagcgcg tagtcgagga tgtgccgcgc gtccgcgagt ttccggaaac
     2221 gtcgtgccgc gtcgaggaca gcttgttcct ctgcggtgag tggcgcgggg gtgctcttct
     2281 tcggcacggt caagctcctg tcttcaagct ccgaaaggtc cggggcggag gaacgcgggg
     2341 agcttgaaag ccgctcggcc gggggatcaa tcccggccgc ctccgccccg gatgatctac
     2401 ttcatcaagg cggcgatggc gcggaccttc tcggttcgct gccgggccga cgcggtcgtc
     2461 ttcgtggcgc ggccggcgag ctgcgcttcg agccgctcgg agagggtcgc ggcgatctcg
     2521 tcgacatccc acgtctcgac gacgtcgcgg agggcgatcc gggtcaacgt cgaggtctcc
     2581 tccgacgtcc ggccttgcca gcgggtccat gagccggtcc agtcgagacg cgcgaaagtc
     2641 ccgtcgtcga ggaggacgag gtcggttccg ccgatgctcc cgtcgttcgc gcgcgggtgg
     2701 tcgcgctccg ggccgttgcc gtcgagccgg acgccgcgct cggggtggta ggtcttctcc
     2761 gtcgaggtct ccgtcgaggt cggccaccat gtcgtctcgc tcgccttcag ccgggacgag
     2821 agggcgcgga gcgcgggcct gacggcgtcg acgagcttct cgaggagttc ctcctcggcg
     2881 gcgcgctcct cgtcgaacgc ggcttccatc gtggcgtgct gctctgcgac cgagcgggag
     2941 aggttggtga tcttctcgac ggcctgctcc atgaagtcct ccttctgtcc gggtcctccc
     3001 cggaggggct tgcgggtccg tcacccgaac gcgccaccgc gacactagcg cagtgtggat
     3061 cttgcgtgaa cgtacatcaa ggggattgag cccttcggac ggctaccaga gcggttttcg
     3121 accgaaagtc cgatgggggg ccaatccgcc gaaaagacgt gtagccggac ttctaagccg
     3181 agggtcgccg gttcgagccc ggccgggcag gccagccgtt cccg
//
LOCUS       DAOONL010000254         3162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_37259_length_3162_cov_18.731574, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000254 DAOONL010000000
VERSION     DAOONL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37259_length_3162_cov_18.731574"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLP50_17745"
     CDS             <1..275
                     /locus_tag="PLP50_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPA53439.1"
                     /translation="QAADDDANIIFGLVQDGGMKEQVKVSVIATGFDAPVAARRAAEK
                     PVERPRLPLAEEAIPEDSGYGVNLVQTRSPKDDIFDIPTFLRRQMD"
     gene            633..956
                     /locus_tag="PLP50_17750"
     CDS             633..956
                     /locus_tag="PLP50_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53440.1"
                     /translation="MTAQKSIPAIALVLVVLSLLFASRPATAACEVCRSDGDNYKLCH
                     QVGDAPGWRNGRTDCVDNHSACFLPGECCYLSQKSEYACSVSYPEGCPNPKECGLKDV
                     QEKDI"
     gene            1555..>3162
                     /locus_tag="PLP50_17755"
     CDS             1555..>3162
                     /locus_tag="PLP50_17755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA53441.1"
                     /translation="MRDRPVVKVEPIVRPRKEEELELAAWTAFYERNGASVASGVLVL
                     DPIPEERERVVFNMLFEGQPGIRLAAAGRLVDGVLDLGTIRLPRRTSLAVSIRSGLPD
                     EEDTGPLTLDLSLVRPVPGLSPPARAPNLSREVRAGSAATFDQLIPGEYRLAVRTPDE
                     TVGSIEVPVAEGNENRLGLELTREAVAGRVEDENGKEIGDALVGLMRSWTRGRSRHEV
                     RTDAQGSFRLEFLHAGGSVSVWTKPTADCATVAVELDPRGPDAAGVALRTRPGGISVR
                     VRDATTKEPIGDARLELDVVLVDGRNGSMHGRTSLPDGVVRFCNLSAGSVRITATARG
                     YEQAVREVEVDADRAAEVELGLERAAQLRGKVLGAAGAPVPGARVFGPLGPLVGFEGE
                     PPSVVCGPDGTFEMDLPTGPSPTLLAVVATGHRLALRWMLPSPEEQPIFLVAAGSDEV
                     VELRYADGVPVRDAWIVLQKDGLLVPGAIVEEAVSAGGCVFEPSSRDGFQRFGGCVEP
                     GTYVLHARLFRPGGFAHLPLGAYQLPLP"
BASE COUNT          457 a         1078 c         1110 g          517 t
ORIGIN      
        1 agcaggcggc cgacgacgac gcgaacatca tcttcggcct cgtccaggac ggcggcatga
       61 aggagcaggt caaggtcagc gtcatcgcga ccggcttcga cgcccccgtc gccgcccggc
      121 gcgccgcgga gaagcccgtc gagcggccgc gcctccccct cgcggaggag gcgatccccg
      181 aggactccgg ctacggagtg aacctcgtcc agacccggag cccgaaggac gacatcttcg
      241 acatcccgac gttcctccgg cggcagatgg actgaggctc ggagccgccc cgcgcccgcc
      301 ctgttgaccc accccgcgcc ccccctgttg acccaccccg cgccccccgc ccaccgccct
      361 tcgcggtgcg cgcttcgcgc ggtctgagat gaagacgggg cgcggggcga agcgttcccg
      421 tcacctgacg cgcgcagaat cccacgccgc agagccggtt ctgcacccgc cggttgcccg
      481 catcccgccc ggttcgtcgc cgagacggcc ccgttcagtt cacgtgtccg cgagagtgcc
      541 tgatttctcg cgctcagggg cgtccctctc ttgtcgccct ccgccccgcc gtcgtaccgt
      601 cgtccgattc tcgaagaaga ggagaactga tagtgacggc tcaaaagagt atccctgcga
      661 tcgccctcgt cctcgtggtc ctgtctttgc tgttcgcgag ccggcccgcg accgcggcgt
      721 gcgaggtgtg ccgcagcgac ggcgacaact acaagctgtg tcatcaggtc ggcgacgcgc
      781 cgggctggcg gaatgggcga accgactgtg tcgacaacca ctccgcgtgc ttccttcctg
      841 gggagtgctg ctacctctcg cagaagtcgg aatacgcgtg ctcggtcagc tatcccgaag
      901 gctgtccgaa ccctaaagag tgcggcctga aggacgtgca ggagaaggac atctagggag
      961 cgcgtggtga tcctggcggc gacggccctc tctgcttccc tcgccctcgc tccggacgag
     1021 accattcgtc ccgaggttcg tctcgacatc tccgcggcgt tcccaagcgg gactccgggc
     1081 gttgttctcg tcgagtcctt ctcgcgcgac ggggcgtcgc tcggacggcg ggaggtgcgg
     1141 cctgcggcga agggggaagg ctgtgccctt cccgcaggaa cggcgcgtgt gcgtgtgtcg
     1201 agcccccttc acgagacctg cgaggccttt gtcgagccgg ccaaggagct cgtctcctgt
     1261 ccgatggctg cccgagcccg gttccatccg tttggcggca gagcaggccg ggtctgggca
     1321 cgacgaaacg agccgggggc gcccttcctg gccatccctc ccctctcgaa agagtggggc
     1381 ggcggcttcg cggttccgcg aggcaggatc gatctcgtcc tggcgccgga tggctcgagg
     1441 ggttctctcc tcaggctggt cgacgccgct tccctcgatg gatcgagaca accggcgaag
     1501 aagagcgatc cgtatcggct caaggtgaga ttcgtcgacg cgagcggggc cgaaatgcgc
     1561 gatcggcccg tcgtcaaggt ggagccgatc gtacggccgc gcaaggagga ggagctggag
     1621 ctcgccgcct ggaccgcctt ctacgagcgg aacggagcct cggtggcgag cggcgtcctc
     1681 gtgctggatc cgattcccga ggaaagggag cgggtcgtct tcaacatgct gttcgaggga
     1741 cagccgggaa tccgcctggc cgccgcgggg cggctggtcg acggggtgct cgacctgggg
     1801 accattcgac tgcctcgccg gacgagtctc gccgtctcca tccgctccgg gctcccggac
     1861 gaggaggata ctggtcctct caccctcgat ctgagcctgg ttcggcccgt gccggggttg
     1921 agtcctcccg cccgggcgcc gaacctgagc cgggaggttc gggccgggtc ggcagcgacc
     1981 ttcgatcagc tcatcccggg ggagtaccgg ctggcagttc gaacgcccga cgaaaccgtc
     2041 ggctccattg aggtccccgt ggccgaaggg aacgagaacc ggttgggcct cgagctgacc
     2101 cgcgaggcgg tcgcggggcg ggtggaggac gagaacggaa aggagatcgg cgacgctctg
     2161 gtcggcttga tgaggtcctg gaccaggggg cgatcgcggc acgaggttcg tactgacgcg
     2221 caggggagct tccggctcga gttccttcat gctggcggat ccgtgagcgt ctggacgaag
     2281 ccgacggccg actgcgccac ggtggccgtc gagctcgacc cgcgcggtcc ggacgcagcc
     2341 ggggtcgccc tccgaacgag gcccgggggg atctccgttc gcgtgcgcga cgccaccacg
     2401 aaagagccga tcggcgacgc tcgcctggag ctcgacgtgg ttttggtcga cggccgaaat
     2461 gggtccatgc acggccggac gagcctcccg gacggtgtcg tccgcttctg caacctctcg
     2521 gcagggtccg tcaggatcac cgcgacggcg cgtggctacg aacaggccgt ccgggaagtc
     2581 gaggtagacg cggaccgggc tgccgaggtc gagcttgggc tcgagcgcgc ggcgcaactt
     2641 cgcggcaagg ttctcggcgc cgcgggagct cccgtcccgg gggcgcgcgt cttcggcccg
     2701 ctcggcccgc tcgtcggttt cgaaggcgag ccgccgtcgg tcgtgtgtgg accggacggg
     2761 acgttcgaga tggatctgcc gacgggaccg tctccgacgc tgctcgccgt cgtggcgacc
     2821 gggcaccgac tcgcgttgag gtggatgctg ccctcaccgg aagaacagcc gatcttcctc
     2881 gtggcggccg gctccgacga agtcgtcgag ctccgatacg ccgacggcgt acccgtccgg
     2941 gatgcctgga tcgtgcttca gaaggacggc cttctcgttc ccggcgcaat cgtcgaggaa
     3001 gcggtttcgg ccggcgggtg cgtcttcgag ccttcgagcc gggacggctt ccagcgcttc
     3061 ggcggatgcg tcgaaccggg aacgtacgtg cttcacgcga ggctcttccg accgggcggc
     3121 ttcgcgcatc tgccgctcgg cgcctatcag ctgccgcttc cc
//
LOCUS       DAOONL010000255         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_37596_length_3140_cov_11.488817, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000255 DAOONL010000000
VERSION     DAOONL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37596_length_3140_cov_11.488817"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLP50_17760"
     CDS             complement(<1..384)
                     /locus_tag="PLP50_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53442.1"
                     /translation="MNAQIGVLVDRDHVLGHSYLMDVATPEALIRRFERQIIPLLQEC
                     FIDDWRRTQQVLGDAEQPPERQVIQQLDRADDGELAHDRKPRFQVSPALTPAAIKKIY
                     TSTTSSGRSARETSTWRSSRRSTAST"
     gene            complement(524..3100)
                     /locus_tag="PLP50_17765"
     CDS             complement(524..3100)
                     /locus_tag="PLP50_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53443.1"
                     /translation="MKTDSLVAQSALLNARRLQQTVARDSPGVWGSSTRLALLHHHPL
                     PLADLSRGPSNRDEYLLLKNAGVFLRELVKLGVDVVLHGHKHSSAIARLVLPTSPNGE
                     DELLVLGVGSVARPGTAELPSYNVVRLYDSGRVVLERRCLRATYEADPPIVLRDYEQQ
                     RPRHSRRLLAESPLTLRAEGLRYHFVIDSNGDAVTESTFTQVRSASESSVTYVQRFLK
                     TRAGRPGNPTYQTLTPGTSIRFSWSGPPNAEGRREGRLEFSPPLRRDPISFIERHPFY
                     NVFCFDQATRSAITGAPTDSEHASYRVEKALETFGMAITFPPYRFPTDFELQVFDSRD
                     RLDLEESDFARRFLHVIPASRTVGFLLASPLPSFRYQIQWRLPAAAAEEFPLTAAQEA
                     MVDRFIAALDEFVGDERDGPSPLAPLLHDLWDRVQNGLQSLGEQTDASLEVHFYVASP
                     DTASIRYASCAGAARKVAHEVISGDSIVGQAFRRRTFLPCVAAHHGDSASPPTYYEPR
                     EVDGGSSALAAYPLLVPSVHGRPVGVLLLVSRAPGSSLCSALSDEARVKDLEEILFLW
                     YRTTLSEAIGLPEVQSAWPSRTASSDTGCPGGLVQAGAGPAAPVQPPASLVRRPRGWR
                     RARRQDVERRLLRLERRVGFAIQETVIRWELLDLEGTVRQTWLHRGVTVREDFALEAI
                     RLGVFTPGPAESVEVEPPTLSYEGVRPGAEYRLRASVNPRGEIEVSLQNARGGRLEID
                     GGSFGVSRVMAHAARVWREDVESAFEKDRIQYEYARAQAISPTRVLKLEVGFPPGYAV
                     QAFPLVFKGSGEVLDDSAARAGALSFEFSGNGGTLEVTDPTDGRFYGIGWTSPSRGDR
                     RA"
BASE COUNT          552 a         1054 c         1029 g          505 t
ORIGIN      
        1 cgtcgaggcc gtcgatcttc gggaggatct ccacgtggag gtctcccgcg cggaccgccc
       61 cgacgaagtg gtggaggtgt agattttctt gatggccgcc ggcgtgaggg ccgggctcac
      121 ctggaaacgc ggctttcggt cgtgcgcgag ctccccgtcg tcggcgcggt cgagctgctg
      181 gatgacctgg cgctccggcg gctgctccgc gtcgccgagg acctgctgtg tgcgccgcca
      241 gtcgtcgatg aagcactcct gcaggagcgg gatgatctgg cgctcgaagc gcctgatcag
      301 cgcctccggc gtggcgacgt ccatcaggta gctgtgcccg agcacgtggt cccgatcgac
      361 cagcaccccg atctgcgcat tcatcgcctt caggttggag cactccttct cctggagatg
      421 ctctcccggg cgccgctgtc agagcccagg ccgggccagg acggaatcgc agcgagaagc
      481 ccttcccgga cggggaagaa tgacgtccga tgaagacctc gcgtcaggcc cgacgatcgc
      541 ccctcgacgg cgaagtccac ccgattccgt agaaacgccc gtccgtagga tcggtcacct
      601 cgagagtccc gccgttccct gagaattcga acgacagagc gcccgctcgc gccgcagagt
      661 catccaggac ctccccactc cccttgaaga ccagcgggaa tgcctgcacg gcgtatccgg
      721 ggggaaaccc gacctccagc ttgagaaccc tcgtcggtga gatcgcttgc gcacgggcgt
      781 actcgtactg aatccggtcc ttctcgaatg cactctccac gtcctcccgc cagacccgtg
      841 ccgcgtgcgc catgacacgc gagacgccga aggaccctcc gtcgatctcc agccttcccc
      901 ctctcgcgtt ctggagagac acctctatct ctcctcgtgg attcaccgac gcgcgcagcc
      961 ggtactcggc gcccgggcga acaccctcgt aagagagcgt cgggggctcc acctccacgg
     1021 attcggctgg gcccggcgtg aagacgccga gccggatcgc ctccagcgcg aagtcctccc
     1081 tgaccgtcac ccctcgatgc agccaggtct gccttaccgt cccctcgagg tccagaagct
     1141 cccaccggat caccgtctcc tggatcgcga acccgaccct gcgctcgagc cggagcagcc
     1201 tgcgctcgac atcctgccgc cgcgctcgtc gccaacctct cggacgccgg accaaggagg
     1261 ccgggggctg gaccggcgca gcgggccccg cgcccgcttg cacgaggccc cctggacagc
     1321 cagtatcgga agaggctgtt ctcgacggcc acgcggactg gacctcgggc aaaccgatcg
     1381 cctcggacaa cgtggttcga taccagagga agaggatctc ctcgaggtcc ttcacgcgcg
     1441 cttcgtccga cagggccgag caaagcgatg agccgggagc cctgcttacg agaaggagca
     1501 ccccgacggg ccgcccgtgc acgctcggga cgagaagggg ataggccgcg agagccgagg
     1561 agccgccgtc gacctcgcga ggctcgtagt aggtcggcgg cgaggcgctg tcgccgtggt
     1621 gagcggcgac gcacggcagg aaggtccttc gccgaaaggc ctgcccgacg atggagtccc
     1681 cggagatcac ctcgtgggcg actttcctcg cagcacctgc acagctcgca taccgaatcg
     1741 aggccgtatc cggcgaggca acgtagaaat ggacctcaag tgacgcgtcg gtctgctcgc
     1801 cgagcgactg caggccgttc tggacacggt cccaaaggtc gtgaaggaga ggcgcgagcg
     1861 gcgagggccc atcccgttcg tcgcctacga actcgtcgag cgccgcgatg aaacgatcga
     1921 ccatggcttc ctgcgccgcg gtgagcggaa actcctccgc agcggcggct ggcaggcgcc
     1981 actggatctg ataccggaac gacggcagcg gactcgcgag caggaaaccg accgtccgcg
     2041 acgcgggaat cacatggagg aaccggcgtg cgaaatcgct ttcttccagg tccagccggt
     2101 ctcggctgtc gaagacctgc agttcgaagt ccgtcgggaa tcggtacggt gggaaggtga
     2161 tcgccatccc gaacgtctcg agcgccttct cgacgcggta ggacgcgtgc tccgaatcgg
     2221 tcggcgcccc ggttatcgcg ctccgagtgg cctgatcgaa gcagaatacg ttgtagaacg
     2281 gatgccgctc gatgaaggag atcgggtcgc gccgaagtgg ggggctgaac tcgagccggc
     2341 cttcgcgacg accttccgcg ttgggaggcc cgctccacga gaagcgaatc gaggtgccgg
     2401 gtgtcagggt ctggtaggtg ggattccccg gtcgccccgc ccgagtcttc aagaatctct
     2461 gcacgtaggt cacggaggac tcggacgccg aacgtacctg agtgaacgtg ctttcggtca
     2521 cggcgtctcc gttcgagtca attacgaagt ggtaccgcag cccctcggca cgcaacgtga
     2581 ggggggactc ggcgaggagc cgcctggagt gccgaggccg ctgctgttcg tagtctctca
     2641 agacgatcgg cgggtcggcc tcgtacgtcg cgcgaaggca cctacgctcg agtacgaccc
     2701 ttccggagtc gtacagccgg acgacgttgt aggaaggaag ctcggcggtt ccgggccgcg
     2761 cgaccgagcc gacgccgagg accagcagct cgtcttcccc gtttggagac gtcggaagga
     2821 ccagacgtgc gatcgccgac gagtgcttgt ggccgtgtag cactacatcc acgcccaact
     2881 tgacaagctc cctcaggaat acgccggcgt tcttcagcag caggtactcg tcccgattcg
     2941 acggtccccg gctcaggtcg gcgaggggca ggggatgatg gtgaagcagg gccagtcgcg
     3001 tagagctacc ccagaccccg ggcgagtccc tcgcaacggt ctgctgcaga cggcgggcgt
     3061 tcaaaagggc cgactgcgca acgagactgt cggttttcac gacctatgga ccccctgacg
     3121 ccgggttttt cacgaccttt
//
LOCUS       DAOONL010000256         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_38327_length_3093_cov_23.379197, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000256 DAOONL010000000
VERSION     DAOONL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38327_length_3093_cov_23.379197"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLP50_17770"
     CDS             complement(<1..941)
                     /locus_tag="PLP50_17770"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA53444.1"
                     /translation="MTNAAPRRRRAAPAAASKAAPAGTRRPARPKRPTPAPPAPIVDG
                     TPHVHPEGEFLWESSPSRYIARMSATTGREVLRPVDPEELLITAHRDKNPFEVTTSFL
                     ASRLVQVTGNEEKVREAMKSANAFGLELAGRLGRLTGSHAVERGAKKLIGVVDRSVFF
                     QFREPTHRTARDVSQRIEKLRGEARAALAARGRSPRLRVLLTGATGFLGQEILAQAAS
                     DRRIAEVVSVIRPQTVRDPKTKEVIRVLTPTERGRLLLSRLGVGGRAARKFRFVEGDI
                     EKPGLGIPAEELATLERTITHVVHCAASVSFDDTYESS"
     gene            complement(1000..>3093)
                     /locus_tag="PLP50_17775"
     CDS             complement(1000..>3093)
                     /locus_tag="PLP50_17775"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPA53445.1"
                     /translation="ACFLDSLATLHLPGYGYGLFYQYGLFRQEIDNGYQKERPDRWLH
                     AAMPWTVERPEEAVLVPVYGRIVDTVDRKSQYNPMWMDWKILVGVPHEMPVVGYGGET
                     VNALRLYSARASDEFNMEFFNSGDYVQAVQESIGSETISKVLYPSDRVQKGKELRLLQ
                     EYFFVACALRDILRRFLREGHAIATFPDRVAIQLNDTHPALAVAELMRLFVDEHDVAW
                     ERAWEMTVATLGYTNHTLLPEALEKWPLPLVETVLPRHLQIIYEVNRRFLDEVAHRAP
                     GDQEVLSRVSMIEEGEPKQVRMAQLAAAGSHSINGVAALHSRLITENLFPDLYRLHPE
                     RFNNKTNGVTPRRWLLKSNPSLAALLTEAAGPGWVTDYGLLREPLDRLAEDAAFRDRF
                     RAVKRENKAHLARIVADLTRVRVDPDSLFDIQIKRIHLYKRQLLNALHVVHLYFRIVE
                     DGETPKAPRTFVFAGKAAPGYWIAKQVIKLIHAVGERVNRDTRVKGMLKCVFLPDYRV
                     SLAERIIPAADLSEQISTAGMEASGTGNMKLSMNGALTIGTLDGANIEIMEEVGEENI
                     FIFGHTAEDLDRLKQPGAWAARSVYEDDPRVRRVLDAFRTPLFAPFEPGIFSWLFENV
                     VENHDPYFHLADFDAYAAAQERVADQWSDAAGWTRKAVLNVARMGKFSSDRTIREYAR
                     EIWRLAPVPPEAPPR"
BASE COUNT          437 a         1026 c         1141 g          489 t
ORIGIN      
        1 gagctctcgt aggtgtcgtc gaaggagacg gacgcggcgc aatggacgac gtgggtaatc
       61 gttcgctcga gcgtcgcgag ctcctccgcg gggatcccga gcccgggctt ctcgatgtcc
      121 ccctcgacga accggaactt gcgcgccgcg cggccgccga cgccgagccg ggagaggagg
      181 aggcgcccgc gctccgtcgg cgtcaggacg cggatcacct ccttcgtctt cgggtcgcga
      241 accgtctggg gccgaatgac ggagacgacc tcggcgatcc tccggtccga cgccgcctgg
      301 gcgaggatct cctggccgag gaagccggtc gcgccggtca ggaggacgcg aagccgcggg
      361 ctccgcccgc gggcggcaag ggccgctcgg gcctcgccgc ggagcttctc gatccgctgc
      421 gacacgtcgc gcgccgtccg gtgggtcggc tcgcggaact ggaagaagac ggagcggtcg
      481 acgacgccga tcagcttctt cgccccccgc tccacagcgt gcgaaccggt gaggcggccg
      541 agccggcccg cgagctcgag gccgaaggcg ttcgccgact tcatcgcctc gcgcaccttc
      601 tcctcgttgc cggtcacctg gacgagccgg ctcgccagga acgacgtcgt cacctcgaac
      661 gggttcttgt cgcggtgcgc cgtgatgagg agctcctccg ggtcgaccgg ccggaggacc
      721 tcgcgccccg tcgtcgcgct catccgggcg atgtaacggg acggcgagct ctcccagagg
      781 aactcccctt cggggtggac gtggggcgtg ccgtcgacga tcggcgccgg gggggcgggc
      841 gtgggtcgct tcggacgcgc cgggcggcgc gtcccggcgg gggcggcttt cgaggcggcg
      901 gcaggagccg ccctgcgtcg gcggggcgcg gcattcgtca ttggactcct ccgggggagg
      961 tgattgtccc ccgaacggcc tccggaaccg aaattcgcgc tagcgcggcg gagcctccgg
     1021 cgggacgggc gcgaggcgcc agatctcccg ggcgtactcg cggatcgtcc ggtcggagga
     1081 gaacttcccc atccgcgcca cgttcagcac cgccttgcgc gtccatccgg ccgcgtccga
     1141 ccactggtcc gcgacccgct cctgcgcggc ggcgtaggcg tcgaagtcgg ccaggtggaa
     1201 gtacgggtcg tggttctcca cgacgttctc gaagagccag gagaagatcc ccggctcgaa
     1261 cggcgcgaag agcggcgtcc ggaacgcgtc gaggacgcgc cggacgcgcg ggtcgtcctc
     1321 gtagacgctt cgggccgccc aagccccggg ctgcttcagc cggtcgaggt cctccgcggt
     1381 gtggccgaag atgaagatgt tctcctcgcc cacctcctcc atgatctcga tgttcgcgcc
     1441 gtcgagcgtc ccgatcgtca gggcgccgtt catcgagagc ttcatgttgc cggtgcccga
     1501 ggcctccatg ccggcggtcg agatctgctc ggagaggtcg gccgccggga tgatccgctc
     1561 ggcgagcgag acccggtagt cgggcaggaa gacgcacttg agcatcccct tgacccgcgt
     1621 gtcccggttg accctctccc cgacggcgtg gatcagcttg atgacctgct tggcgatcca
     1681 gtagcccggc gcggccttgc cggcgaagac gaacgtccgc ggcgccttcg gcgtctcccc
     1741 gtcctcgacg atccggaagt agaggtggac gacgtggagg gcgttcagga gctgccgttt
     1801 gtagaggtgg atccgcttga tctggatgtc gaagagggag tcggggtcga cccgcacgcg
     1861 cgtcaggtcg gcgacgatcc tcgcgaggtg ggccttgttc tcgcgcttga cggcgcggaa
     1921 gcggtcgcgg aacgcggcgt cctcggcgag cctgtcgagc ggctcgcgca ggaggccgta
     1981 gtcggtcacc cagccggggc cggcggcctc ggtgaggagg gcggcgaggc tcgggttcga
     2041 cttcagaagc cagcgccgcg gcgtgacgcc gttcgtcttg ttgttgaacc gctccgggtg
     2101 gagccggtag aggtcgggga agaggttctc ggtgatgagg cgggagtgaa gcgcggcgac
     2161 gccgttgatc gagtggctcc ccgccgccgc gagctgagcc atccggacct gcttcggctc
     2221 tccctcctcg atcatcgaga cgcgggagag gacctcctgg tctccgggtg cgcggtgcgc
     2281 gacctcgtcc aggaaacgcc ggttgacctc gtagatgatc tgcaggtgcc gcgggaggac
     2341 cgtctccacg agcgggagcg gccacttctc gagcgcctcc ggcagcagcg tgtggttcgt
     2401 gtagccgagc gtggcgacgg tcatctccca cgcccgctcc cacgccacgt cgtgctcgtc
     2461 gacgaagagg cgcatcagct cggccacggc cagggcgggg tgcgtgtcgt tcagctggat
     2521 cgcgacccgg tcggggaacg tcgcgatcgc gtgcccctcg cggaggaagc ggcggaggat
     2581 gtcgcgcagc gcgcaggcga cgaagaagta ctcctgcagg aggcggagct ccttgccctt
     2641 ctgcaccctg tcggaggggt agaggacttt ggagatcgtc tccgacccga tcgactcctg
     2701 caccgcctgg acgtagtcgc cggagttgaa gaactccatg ttgaactcgt cggaggcgcg
     2761 agccgagtag agccggagcg cgttcaccgt ctcgccgccg tagccgacga ccggcatctc
     2821 gtgcggcacg ccgacgagga tcttccagtc catccacatc gggttgtact ggctcttccg
     2881 gtcgaccgtg tcgacgatcc ggccgtagac cgggacgagg acggcctcct cgggccgctc
     2941 gaccgtccag ggcatcgcgg cgtggagcca gcggtcgggg cgctccttct ggtagccgtt
     3001 gtcgatctcc tggcggaaga ggccgtactg gtagaagagg ccgtagccgt agcccggcag
     3061 gtggagcgtc gcgagcgagt cgaggaagca ggc
//
LOCUS       DAOONL010000257         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_38620_length_3076_cov_18.223105, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000257 DAOONL010000000
VERSION     DAOONL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38620_length_3076_cov_18.223105"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLP50_17780"
     CDS             <1..849
                     /locus_tag="PLP50_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPA53446.1"
                     /translation="DCILKIRTLLGFLPSNNRQDPPRRECADPLDREDAELDTLVPAS
                     PNQPYDIKDVIRRVVDDGELFEIHADFARNLVVGFARLGGRPVGIVANQPDFLAGVLD
                     IDSSTKGARFVRFCDAFNVPLVTFEDVPGFLPGTQQEFGGIIRHGAKLLYAFAEATVP
                     KVTVITRKAYGGAYCVMSSKHIRTDFNYAWPTAEIAVMGPEGAVNVLYGRELARSADP
                     ATDRRAKVEEYQDLFANPYVAAQKGYVDDVIFPRTTRRRLIRALDLIENKRDVLPPKK
                     HGNMPL"
     gene            968..>1263
                     /locus_tag="PLP50_17785"
     CDS             968..>1263
                     /locus_tag="PLP50_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPA53447.1"
                     /translation="MRLLIANRGEIAVRIARACRDLGISPVAVFSDADRAALHVRLSD
                     HAVGIGPAPARESYLVPEKLIDAAKRTGASLVHPGFGFLAENAAFARAVRDAGLV"
     assembly_gap    1264..1363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1364..2017
                     /locus_tag="PLP50_17790"
     CDS             <1364..2017
                     /locus_tag="PLP50_17790"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPA53448.1"
                     /translation="PDGRFFFLEMNTRIQVEHPVTEMVYGVDLVAEMIRIARGEPMSV
                     AARPEPRGHALECRIYAEDPAHGFAPSPGTIERLRWPQGPGVRVDSGVEEGDVIPLDY
                     DPMVAKVVTHGRDRDEALRRMRRALREARIDGIETSIPFFLSLLEDADVLSANVSTQF
                     LESFTFEPPAPSADAEEIGLVAAAVAAARASLALRGASQPRPPSAWRTARPTYGHRE"
     gene            2014..2541
                     /locus_tag="PLP50_17795"
     CDS             2014..2541
                     /locus_tag="PLP50_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPA53449.1"
                     /translation="MKYRARLSGPAGERTVEVEVQLDPAGVVVTLDGRPARWDVVPTP
                     GGGASLLSADGRQAEAVPGRTAEGAILVRVGAERVALELLDELTARAQAVAGTGAARG
                     SGDVKAAIPGRVLRLLVAPGDEVRRGQPVVVLEAMKMENEVRAPRDGVVASVEVAAGQ
                     PVGAGQLLLRLEPVG"
     gene            2687..>3076
                     /locus_tag="PLP50_17800"
     CDS             2687..>3076
                     /locus_tag="PLP50_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53450.1"
                     /translation="MKHHRSLVLFAVTALLACRAGGTPQEAAPAKPATPPAAAAPSPA
                     AAPAAASPAPKPADPRTEALVAWLGEYFPWGPGELKVEPIDAVRVPGWRLYRTMKLFE
                     GDQRANDQAFLLADDAGKTVLAGDIFVE"
BASE COUNT          408 a         1105 c         1059 g          404 t
ORIGIN      
        1 gactgcatcc tgaagatccg gacgctcctc ggcttccttc cctcgaacaa ccggcaggac
       61 ccgccgcggc gggagtgcgc cgaccctctc gaccgcgagg acgcggagct cgacacgctc
      121 gtccccgcga gcccgaacca gccatacgac atcaaggacg tgatccggcg ggtcgtcgac
      181 gacggcgagc tgttcgagat ccacgccgac ttcgcgagga acctcgtcgt cggattcgcg
      241 cggctcggcg ggcgccccgt cgggatcgtc gcgaaccagc ccgacttcct cgccggcgtc
      301 ctcgacatcg actcctcgac gaagggggcg cgcttcgtcc gcttctgcga cgctttcaac
      361 gtccccctcg tcaccttcga ggacgttccg ggcttcctcc ccggcaccca gcaggagttc
      421 ggcgggatca tccggcacgg cgcgaagctc ctttacgcgt tcgccgaggc gaccgtcccg
      481 aaggtcaccg tcatcacgcg gaaggcctac ggcggcgcgt actgcgtcat gtcgtccaag
      541 cacatccgga ccgacttcaa ctacgcctgg ccgacggcgg agatcgccgt catgggaccg
      601 gagggggcgg tgaacgtcct ctacggccgc gagctcgcgc ggagcgccga ccccgccacg
      661 gaccgccggg cgaaggtcga ggagtaccag gacctcttcg cgaaccccta cgtcgccgcg
      721 cagaagggct acgtcgacga cgtcatcttt ccgcgcacga cccgccgacg cctcatccgc
      781 gccctcgacc tcatcgagaa caagcgcgac gtccttcctc ccaagaaaca cgggaacatg
      841 ccgctttgac gcgatcagcg ccgaatgtga ggggaagggg tctggtctac actctacagt
      901 ctgcgccctc cgggacgccc gtagagtgga attccgaggg gcctgacccc cactctacgg
      961 ggtgcgcgtg aggctgctga tcgcgaaccg gggcgagatc gcggtgcgga tcgcgcgggc
     1021 ctgccgggac ctcgggatct cccccgtcgc ggtcttctcg gacgcggacc gcgccgcgct
     1081 gcacgtccgg ctctccgacc acgcggtcgg gatcgggccg gcgccggcgc gcgagtcgta
     1141 cctcgtgccg gagaagctga tcgacgcggc gaaacggacc ggcgcgagcc tcgtccatcc
     1201 ggggttcggc ttcctcgccg agaacgccgc gttcgctcgg gccgtccgcg acgcggggct
     1261 cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgacg gccgcttctt
     1381 cttcctcgag atgaacacgc ggatccaggt cgagcacccg gtgaccgaga tggtctacgg
     1441 cgtcgacctc gtcgccgaga tgatccggat cgcgcgcgga gagccgatga gcgtcgcggc
     1501 gcggccggag ccgcgggggc acgccctcga gtgccggatc tacgcggagg accccgcgca
     1561 cggcttcgcg ccgtcgcccg ggacgatcga gcgtctccgc tggccgcagg ggcccggcgt
     1621 ccgcgtcgac tcgggcgtgg aggaggggga cgtcattccg ctcgactacg acccgatggt
     1681 cgcgaaggtc gtgacgcacg gccgcgaccg cgacgaggcg ctccgcagga tgcgacgggc
     1741 gcttcgggaa gcgcgcatcg acgggatcga gacgtcgatc ccgttcttcc tctccctcct
     1801 cgaggacgcc gacgtcctct cggcgaacgt ctccacgcaa ttcctcgagt cgttcacgtt
     1861 cgagccgccc gccccctccg ccgacgcgga ggagatcggt ctcgtcgccg cggcggtggc
     1921 ggcggcgcgg gcgagcctcg ccctgcgcgg ggcctctcag ccgcgcccgc cctccgcctg
     1981 gcggaccgcc cgcccgacct acggacaccg agaatgaagt accgcgcgag gctctccggg
     2041 ccggccgggg agaggaccgt cgaggtcgag gtgcagctcg acccggccgg cgtcgtcgtg
     2101 acgctcgacg gccggccggc gcgctgggac gtcgtcccga cgcccggcgg aggcgcgtcg
     2161 ctcctctccg ccgacggccg gcaggccgag gcggtgccgg ggcggacggc cgagggggcg
     2221 atcctcgtcc gggtcggggc cgagcgcgtc gcgctggagc tcctcgacga gctgacagcg
     2281 cgcgcgcaag ccgtggccgg aacgggagcc gcccggggct cgggcgacgt gaaggcggcg
     2341 atcccgggtc gtgtcctccg gctcctcgtc gcgccgggtg acgaggtgcg gcgcggccag
     2401 ccggtcgtcg tcctggaggc gatgaagatg gagaacgagg tgcgcgcccc gcgggacggc
     2461 gtcgtcgcct cggtcgaggt cgccgccggg cagccggtcg gcgccgggca gctcctcctg
     2521 cgcctcgagc cggtcggctg aacgccggag cgcggcacgc ccttcgctat catcccggcc
     2581 gcatgccggt tagacgtctt cggcccgtgg gaacggcgca ccgcgcgctc cggccggccg
     2641 ccggcaggtc cacagtccag ctcgacagag gaggctcgtc cgtctgatga agcaccaccg
     2701 ctccctcgtc ctattcgccg tcacggcgct gctcgcgtgt cgggccggcg ggacgccgca
     2761 ggaggccgcg cccgcgaagc cggccacgcc tccggcggcc gccgcgccgt ccccggcggc
     2821 cgccccggcc gcggcgtctc cggcgccgaa gccggccgac ccgaggaccg aggcgctcgt
     2881 cgcctggctc ggagagtact tcccctgggg accgggtgag ctgaaggtcg agccgatcga
     2941 cgccgtccgg gtcccgggct ggcgcctcta ccggacgatg aagctcttcg agggcgacca
     3001 gagagcgaac gaccaggcgt tcctcctggc ggacgacgcc gggaagacgg tcctcgccgg
     3061 cgacatcttc gtcgag
//
LOCUS       DAOONL010000258         2949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_40934_length_2949_cov_22.550104, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000258 DAOONL010000000
VERSION     DAOONL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40934_length_2949_cov_22.550104"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLP50_17805"
     CDS             complement(<1..464)
                     /locus_tag="PLP50_17805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53451.1"
                     /translation="MSAIQSTAERPVFGSITLKAVMALTGIVLYGFVFVHMVGNIQLY
                     QGPEKINGYAAFLKSVPPLLWGTRITLLAAVLLHALAAFTLWRRNLAARPVGYAHQSF
                     QAAGITSRTMYWTGPVIALFVIYHLLHFTLGSAHSQFSHTDVYTNVVVGFSNV"
     gene            complement(472..1398)
                     /gene="mdh"
                     /locus_tag="PLP50_17810"
     CDS             complement(472..1398)
                     /gene="mdh"
                     /locus_tag="PLP50_17810"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPA53452.1"
                     /translation="MRRKITVIGAGNVGATAAQRIVEKELGDVVLVDVVDGVPQGKGL
                     DMYQSGAVEGFDMKIVGSNGYEETADSDLCIMTAGLPRKPGMSRDDLLKTNEAIVADC
                     IGKVTRYSRDLTIIVVTNPLDAMCEVARRVSKLPKNKVIGMAGILDSARMRAFIAMEM
                     NVAMSNIHATVLGGHGDTMVPLPRYSTVAGVPITELLPPERVEAIVKRTADGGAEIVK
                     LLKTGSAYYAPSAAAVEMADAIFNDKKKILPCAALLEGEYGIDGLYVGVPVVLGKDGV
                     EKIIELKLTPEEAAALRKSADSVAELCGKLTV"
     gene            1546..1896
                     /locus_tag="PLP50_17815"
     CDS             1546..1896
                     /locus_tag="PLP50_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPA53453.1"
                     /translation="MDVRETPAWRKAIGLAHEVCATLEDAADLPGDSGQRLRRAALAV
                     PSLVADAFLDTGAADCRERLSAAAARVAELRQLLRAPEIRRFVPGPEADALLSEAEAL
                     ALEIRALGGRCPEA"
     gene            1956..2303
                     /locus_tag="PLP50_17820"
     CDS             1956..2303
                     /locus_tag="PLP50_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53454.1"
                     /translation="MTLRPLAAILAAFVLLTAPCALAQDAGAPAPETTAAAAATTEAA
                     PATTETAPAEAPATTTEAAPAGHEGDHAAAPAPAATAEVPVGTSQTTTIVIGLIVLLL
                     LIVLVILGTKKQE"
     gene            complement(2675..>2949)
                     /locus_tag="PLP50_17825"
     CDS             complement(2675..>2949)
                     /locus_tag="PLP50_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53455.1"
                     /translation="TMATMAGAAMDVLGGLFGKKKSIRVSKVGSVLSKRRMEGAAETK
                     IETLKAEIEELESKVAAPDPARFERVDVVPTKTGVDLLGVGVAWVS"
BASE COUNT          424 a         1046 c         1033 g          446 t
ORIGIN      
        1 acgttcgaga agccgacgac gacgttcgtg tagacgtcgg tgtggctgaa ctgcgagtgg
       61 gccgagccga gcgtgaagtg caggaggtgg tagatcacga agagggcgat cacgggaccg
      121 gtccagtaca tcgtccggga ggtgatcccg gccgcctgga agctctggtg ggcgtagccg
      181 accgggcggg ccgcgaggtt gcgccgccag agcgtgaacg ccgccaaggc gtggaggagg
      241 acggccgcga ggagagtgat ccgggtcccc cagaggaggg gcgggaccga cttcaggaac
      301 gccgcgtagc cgttgatctt ctcgggaccc tgatagagct ggatgttgcc gaccatgtga
      361 acgaagacga agccgtagag gacgatgccg gtcagggcca tcaccgcctt gagcgtgatg
      421 gagccgaaga ccggacgttc cgccgtggac tggatcgcgc tcatcgttcc gtcagacggt
      481 cagcttcccg cagagctcgg cgacggagtc ggcggacttc cggagggcgg cggcctcctc
      541 gggcgtcagc ttcagctcga tgatcttctc gacgccgtcc ttgccgagga cgaccgggac
      601 gccgacgtag aggccgtcga tgccgtactc cccctcgagg agcgccgcgc aggggaggat
      661 cttcttcttg tcgttgaaga tcgcgtcggc catctcgacg gccgcggccg acggggcgta
      721 gtaggccgag ccggtcttca ggagcttgac gatctcggcg ccgccgtcgg cggtccgctt
      781 cacgatcgcc tcgaccctct cgggcgggag gagctcggtg atcgggacgc ccgcgaccgt
      841 cgagtaccgc ggcagcggga ccatcgtgtc accgtgcccg ccgaggaccg tcgcgtggat
      901 gttcgacatc gcgacgttca tctccatggc gatgaaggcg cgcattcggg ccgagtcgag
      961 gatgccggcc atcccgatga ccttgttctt cgggagcttc gagacccgcc gggcgacctc
     1021 gcacatcgcg tcgagggggt tcgtgacgac gatgatcgtc aggtcgcggg agtagcgggt
     1081 caccttgccg atgcagtcgg cgacgatcgc ctcgttcgtc ttgaggaggt cgtcgcgcga
     1141 catgccgggc ttgcggggaa ggccggccgt catgatgcag aggtccgagt ccgccgtctc
     1201 ctcgtacccg ttggagccga cgatcttcat gtcgaagcct tcgacggcgc ccgactggta
     1261 catgtcgagg cccttgccct ggggaacgcc gtcgacgacg tcgacgagga cgacgtctcc
     1321 gagctccttc tcgacgatcc gctgggcggc ggtcgcgccg acgttcccgg cgccgatgac
     1381 agtgatcttc cgtcgcatgc ttctccccct gctctcgaag attccggcca cggccgggac
     1441 tgtactgatc cgaccgggaa gcgtagcagg ggggggaagc ccccgaaagg ggagccccga
     1501 gcggaacgac cgggccccca cgtccgtata tgcttcaggt tggagatgga cgtacgggag
     1561 acccctgcct ggcggaaggc catcggcctc gcccacgagg tctgcgcgac gctcgaggac
     1621 gcggcggacc tccccggcga ttccgggcag cgccttcggc gcgccgctct ggccgtaccg
     1681 tccctcgtcg ccgacgcctt cctcgacacc ggcgccgcgg actgccggga gcggctcagc
     1741 gcggccgcgg cacgggtcgc cgagcttcga cagctccttc gggcgcccga gatccgccgg
     1801 ttcgtcccgg gcccggaggc cgatgccctc ctctccgagg cggaggcgct ggcgctcgag
     1861 atccgggcgc tcggcgggcg ctgccccgag gcctgaccct tcccgatttc ccctccgcct
     1921 gctagcgttc ccccgctgga aggaaaggag acccgatgac gctccgcccg ctcgccgcga
     1981 tcctggcggc tttcgtcctc ctcacggccc cctgcgccct cgcccaggac gccggcgccc
     2041 cggccccgga aacgaccgcc gcggccgccg cgacgaccga ggccgccccg gcgacgacgg
     2101 agacggctcc cgccgaggct ccggcgacga cgaccgaggc cgcgcccgcc ggccacgagg
     2161 gcgaccacgc cgccgccccg gcccccgccg cgacggcgga ggtccccgtg ggcacctccc
     2221 agacgacgac gatcgtcatc ggcctcatcg tcctcctcct cctgatcgtc ctcgtcatcc
     2281 tcggtacgaa gaagcaggag tagggaggag ccccgggaga ccgggcagcc cgactgccga
     2341 gaccggggca ccggggcatc ggagcaccag agcaccagag caccggggag ggaggctcgc
     2401 gccgggggag ggtctcgccg ccgctcgccg gggggctgag cttcgcgcgc cccccggacc
     2461 cccactctct cctcaactca cactgccatt tttcaagctt cctggctgcg cgcggccgac
     2521 ccgctcgacc tcgcgaggat cgggcgggtc ggccgcgcgc agctggaggc cgcgggacgc
     2581 cccgccccga gcgcggcccc ctgcggaatc gacgagagcc tgcggctggt aggttctccg
     2641 gttctccggt tccggggtgc cctcttcccc ccggtcagct cacccaggcg acgccgacgc
     2701 cgagaaggtc gactccggtc ttcgtcggga cgacgtcgac cctctcgaag cgggccgggt
     2761 cgggggcggc gaccttcgac tcgagctcct cgatctcggc cttgagggtc tcgatcttcg
     2821 tctcggcggc gccttccatc cggcgcttgc tgaggaccga gccgaccttc gagacgcgga
     2881 tcgacttctt cttgccgaag aggccgccga ggacgtccat cgccgcgccg gccatcgtgg
     2941 ccatcgtct
//
LOCUS       DAOONL010000259         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_41895_length_2900_cov_10.533919, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000259 DAOONL010000000
VERSION     DAOONL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41895_length_2900_cov_10.533919"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2900
                     /locus_tag="PLP50_17830"
     CDS             <1..>2900
                     /locus_tag="PLP50_17830"
                     /inference="COORDINATES: protein motif:HMM:NF037509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53456.1"
                     /translation="AAREAWEAENRPAPPMPEAPAPAPPAGTDADIERWKAQGEALDA
                     RLQGLREPGATRAPEAAVVDPEGRRVVPQETIGAINEGVRELVPQEAPARPGLLDDVL
                     ARTERPNVLGDVLGATRARDRAAQAAEQRFRAEADSAERQRIAETPGAALRLILGDDP
                     VFLQNDPQPVGVGGSARPEAAPGEGNPAGVPPPVRRRGGTEPGGTPGAPPAGPASLPP
                     PVAPAAAAARSFALPEGWTSEQGNASFPLVRGKVAAITPEGHVGPYRDTLEESYRETA
                     KMREEAAARHEAAARHESVVRAVAAAGKATPEEIRSLGRGRDRLSRSAVRALADDFGL
                     RGKEVQRLLNDGALGIYGDTKGGATLHSTAEVVERILYHLSKRPAPARAPGRKAPVVL
                     GPSAAAASAEAPVPPVAPGAAERGTPGDVPNPAPSMDAFPHRGAMGSTWTADGERVDF
                     QWALVDLAGDVVVSHRRSEPTLPLEENPDFPQGLQPRDRERVASAMQIDDIKRNLEPL
                     RLEESAEASNGAPILGPDRVTESGNGRLIALEELYREGKAGPYTDRLKATAASRGLPV
                     AEVERMRWPAAVRVRTSPTTDRAELVKKFNAPTVAGMAASETAEADGDRLSPDLLSRL
                     HVGENGVDLLSAANGPFVRAFLAKLTPNEAVTLVTADGVLNKDGRRRIEQALVGRAFG
                     RSAVLEKITESADDNTRLVSSAILNSAPRMADVRAAIEQERLPRALDPTPAIEEAVQK
                     LTWLREEGQSVEGYLQQSGLFGDTMTPAGRAVLAAFGEFARSGKKTTAFLTQLGHGLM
                     LEANEKLLSDLESPPLADFVGAARRFVSDGGQSTLFGGPDLSAPDPVQPDSGGDGERG
                     PSGPVDGGRRSARAGADAPAVADARHVLLVQRWTTQLSERWKDSPEIRVVSAEAELPE
                     GVRSRAEEFGATGTIEGLYTGPEGDLVYVVAPMVKSKADAERIVLHEVMGHAG"
BASE COUNT          445 a          965 c         1117 g          373 t
ORIGIN      
        1 gcggcccgcg aggcgtggga ggccgagaat cggccggcgc cgccgatgcc ggaggctccc
       61 gccccggcgc ctccggcggg aacagacgcc gacatcgagc ggtggaaggc ccagggcgag
      121 gctctcgacg cgcggctcca ggggctccgg gagcccgggg cgacgcgggc accggaggcg
      181 gcggtggtgg acccggaggg ccggcgcgtc gttccgcagg agaccatcgg cgcgataaac
      241 gagggcgtcc gggagctcgt gccgcaggag gcgccggcga ggccgggcct cctcgacgac
      301 gtcctcgcgc ggacggaacg cccgaacgtg ctgggcgacg tcctcggtgc gacccgcgcg
      361 cgcgatcgag cggcgcaggc tgcggagcag cgcttccgcg cggaggccga ctcggcagag
      421 cggcagcgga tcgcggagac accgggggcg gcgctccggc tcatcctcgg ggacgacccg
      481 gtcttccttc agaatgaccc tcagcccgtg ggagtgggcg gcagcgcgcg gcctgaagcc
      541 gctcccgggg agggaaatcc cgccggagta ccgccacctg ttcgacggcg cggcggcacc
      601 gagcccggag gaacccccgg agcccctccc gccggccccg cttcccttcc gcccccggta
      661 gcgccggctg cggccgcggc gcgctcgttc gctctccccg agggctggac gtccgagcag
      721 gggaacgcga gtttcccgct ggtccgaggg aaggtcgcgg cgatcacgcc ggaggggcac
      781 gtcgggccgt accgcgacac gctcgaggag tcctaccgcg agaccgcgaa gatgcgtgaa
      841 gaggcggctg cccggcacga ggcggcggcc cgtcacgaga gcgtcgtccg ggcggtggca
      901 gcagcaggga aggcgacccc ggaggagatc cgttccctgg gccgcgggcg ggaccgactt
      961 tcgcgctctg cggtgcgtgc gctggcggac gacttcgggc tccgcggcaa ggaggtccag
     1021 aggctcctca acgacggggc gctcgggatc tacggcgaca cgaagggggg cgcgacgctg
     1081 cactcgactg cggaagtggt cgaacgcatc ctgtaccacc tttcgaagcg cccggctccg
     1141 gcgcgcgcgc caggccggaa ggcgccggtg gtgctggggc cttctgcggc cgcggcgtct
     1201 gccgaagcgc ccgttcctcc ggtagctccc ggagctgccg agcgcggaac gcccggagat
     1261 gtcccgaacc ccgccccgag catggatgcg ttccctcatc gcggggcgat ggggagcacc
     1321 tggacggcag acggcgagcg ggtcgatttc cagtgggcgc ttgtggacct cgcgggggac
     1381 gtggtggtgt ctcatcggcg gtccgagccc accctccccc tcgaggagaa ccccgatttc
     1441 ccccaggggc tccagccgcg tgaccgcgag cgggtagcgt cggcaatgca gatcgacgac
     1501 atcaagcgga acctcgagcc gctccggctc gaggagagcg ccgaggcgtc gaacggcgcg
     1561 ccgatactcg gccccgaccg cgtaacggag agcggaaacg ggcgcctgat cgcgctcgag
     1621 gaactctacc gcgaaggaaa ggccggcccg tacacggatc ggctgaaggc gacggcggct
     1681 tcgcgcggcc tgccggttgc cgaggtggag cggatgcgct ggccggctgc ggtgcgggtg
     1741 aggacctccc cgacgaccga ccgggcggag ctggtgaaga agttcaacgc cccgacggtt
     1801 gccgggatgg cggcgagcga gacggcagag gcggacggcg accggctctc cccggatctt
     1861 ctctcgaggc tccatgtcgg ggagaacggg gtggatctgc tctcggccgc gaacggcccg
     1921 ttcgtgcgtg cgttcctggc gaagctcacc ccgaatgagg cggtaacgct ggttacggcg
     1981 gacggcgtcc tgaacaagga cgggcgcagg aggatcgagc aggcgctcgt tgggcgggcc
     2041 ttcgggcgct ccgccgtgct cgagaagatc acggagagcg cggacgacaa cacgcggctc
     2101 gtctcgtctg cgattctcaa ctcggcgcca cgaatggccg acgtgcgcgc cgcgatcgag
     2161 caggagcgcc tcccgcgcgc gctcgatccg acgccggcca tcgaagaggc cgtccagaag
     2221 ctcacctggc tccgcgagga agggcagagc gtcgaagggt atctccagca gagtggcctc
     2281 ttcggcgaca cgatgacgcc ggctggacgc gcggtgctgg ccgctttcgg agagttcgca
     2341 cgctccggga agaagacgac agccttcttg acacagctcg ggcacgggct catgttggaa
     2401 gcgaatgaga agctcctttc ggatctcgaa agcccgccgc tcgctgactt cgtcggtgca
     2461 gcccggcgct tcgtttcgga cggaggacag agcactctct tcggaggacc tgatctatcg
     2521 gcgcctgatc cggtacaacc ggattcggga ggcgatggag agagaggacc ttccggtcca
     2581 gtagacggcg gcaggcgcag cgcccgggcc ggtgcggatg cgcccgcggt tgctgacgcc
     2641 cggcacgtcc tcctcgtcca gcggtggacg actcagcttt ccgagcggtg gaaggactcg
     2701 ccggagatcc gtgtggtctc cgcggaggcc gagctcccgg agggcgtccg gtcgcgggcg
     2761 gaggagttcg gggcgacggg cacgatcgag ggcctctaca ccgggccgga aggggacctc
     2821 gtctacgtcg tcgcgccgat ggtgaagagc aaggccgacg cggagcggat cgtcctgcat
     2881 gaggtcatgg ggcacgcggg
//
LOCUS       DAOONL010000260         2750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_45164_length_2750_cov_13.125788, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000260 DAOONL010000000
VERSION     DAOONL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45164_length_2750_cov_13.125788"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLP50_17835"
     CDS             <1..485
                     /locus_tag="PLP50_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53457.1"
                     /translation="AALGETLRRRSPAGYAVVLVLVLSPLAIGRSVGTHSITARLFPE
                     PPLADSNLDWGQDLPRLAALTAARGIAPGDLGVAYFGGDLPSFRVPGCVDLLAADAPL
                     PRFVAVSRQLLLVGPETVPFADGRSRAARVLAELKDRRARVVARAGDSIELFELPAPR
                     "
     gene            complement(714..2411)
                     /locus_tag="PLP50_17840"
     CDS             complement(714..2411)
                     /locus_tag="PLP50_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53458.1"
                     /translation="MTGSKLLRSAAVVAALFLSARAHGTTITIVNGDAPGLGFNDPSP
                     RTPVGGNTGTTLGEQRLKVFEYAAAFWAERLDSPVEIRVRAKFEALTPCTTTSAVLGS
                     AGTTQVWSDFRGAPRAETWFPVALANKLAGEDLDPDDEDVRATFTSSLDDGSCAYPKK
                     WYYGLDAQPTSGTTDFATVIIHELGHGLGFQTFVDRETGQKFPYGDPQAMDDAYMVHL
                     YDGTTGKTWDQMTDGERLASSTNTGNLLWNGPIVIAAASGVLIQGIGAGGRPLMYAPS
                     LSEAGSSISHWDTSLSPNEMMEPYYTSAMHRLDITDELMLDLGWGLASGSAEYHWILP
                     SSAKAPGKGGAYYTTALSIGNRGAAEARYRMKFLGHDADGTGGAESSEFVLGPNQSVT
                     YEDVLGSVFSLANGNYGAIRITANVPSLNVLGQTATPVLTDWKSCIASGFFGQSVPAV
                     PASGLITNGTTRSIVGVRDDAAFRTNLILANAGTSIVTVDLTLLSASGAILGTRSVGL
                     HPLGMTQVGPLVGAMGVTGAVRDAQLLLTTSTTGGAFAAYASVIDNVTNDPRTLLPN"
     gene            complement(2398..>2750)
                     /locus_tag="PLP50_17845"
     CDS             complement(2398..>2750)
                     /locus_tag="PLP50_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53459.1"
                     /translation="PVAATEKKSEAPRPAPAAEAAMKAHIDPVTGEFVQKPGLSPKTA
                     AEASPVTSLPMPRVELIDSKRGTKRVILDRRFDSQMVDTVGPERKVSLTSAEQPATAG
                     QPPAPETVRRHDRQ"
BASE COUNT          391 a          908 c          984 g          467 t
ORIGIN      
        1 ccgccgcgct cggggagacc ctgcggcgcc gctcgcccgc cggctacgcc gtcgtcctcg
       61 tcctcgtcct ttccccgctc gcgatcggac gctcggtcgg cacgcactcg atcacggccc
      121 gcctcttccc ggagccgccg ctcgcggact cgaacctcga ctggggccag gacctgccgc
      181 ggctcgccgc gctcaccgcc gcgcgcggga tcgcgccggg cgacctcggc gtcgcctact
      241 tcggcggcga cctgccgtcg ttccgcgtcc cgggctgcgt cgacctcctc gccgccgacg
      301 cgccgctccc gcgcttcgtc gccgtctccc gccagctcct cctcgtcggg ccggagacgg
      361 tcccgttcgc cgacgggcgg tcgagagcgg cgcgcgtcct cgcggagctg aaggatcgcc
      421 gggcgcgcgt cgtggcgcgc gcgggcgact cgatcgagct cttcgagctg ccggcgccgc
      481 gctgagccga gaaacctcag ttcgcgcgta agtgaatgca gtcacgcacg attccaactg
      541 tggcgcagtt gaaacgggtc acgcgaagtg ctgcacaggc aaagcgttac caccgaaagc
      601 gtcgtcaact ggggtttctc ggctgaggcg cggtcgccga aagcggaaga gccccggtcc
      661 gcgaaagcgg gccggggctc ttcgtcgcgc ggatcgccgg cgacgaagac gggtcagttg
      721 gggaggagcg tcctcgggtc gttcgtgacg ttgtcgatga cgctcgcgta ggcggcaaag
      781 gcgccgccgg tcgtggaggt cgtcaggagg agctgtgcgt cacggacggc tccggtgacg
      841 cccatcgccc cgacgagcgg gccgacctgc gtcatcccga gcgggtgaag gccgacgctg
      901 cgggtcccga ggatcgcgcc ggaggccgag aggagggtca ggtcgaccgt cacgatcgac
      961 gtgccggcgt tcgcgaggat gaggttcgtc cggaatgcgg catcgtcgcg gacgccgacg
     1021 atggaccgcg tcgtgccgtt ggtgatgagg ccggaggccg gtacggccgg gaccgactgc
     1081 ccgaagaagc ctgaggcgat acagctcttc cagtccgtga gaacgggcgt cgccgtctgc
     1141 ccgaggacgt tcagcgacgg gacgttcgcc gtgatgcgga tcgcgccgta attgccgttc
     1201 gcgaggctga agacggagcc gaggacgtcc tcgtacgtga ccgactggtt cggcccgagg
     1261 acgaactcgg acgactcggc gccgccggtg ccgtcggcgt catgcccgag gaacttcatc
     1321 cggtagcgcg cctcggcggc gccgcggttc ccgatcgaga gggccgtcgt gtagtaggcg
     1381 cctcccttgc ccggggcctt cgcggaggag gggagtatcc agtgatactc ggcgctcccg
     1441 gaggcgaggc cccagccgag gtccaacatc agctcgtcgg tgatgtcgag ccggtgcatc
     1501 gcggaggtgt agtacggctc catcatctcg ttcgggctca gggacgtgtc ccagtgggag
     1561 atggaggacc cggcctcgga aaggctcggg gcgtacatca acggcctgcc acctgcgccg
     1621 attccctgga tcaggacgcc cgaggcggcc gcgatgacga tcggcccgtt ccagaggagg
     1681 ttccccgtgt tcgtgcttga tgcgagccgc tcgccgtccg tcatctggtc ccacgtcttg
     1741 ccggtcgtgc cgtcgtagag gtggaccata taggcgtcgt ccatcgcctg cgggtcgccg
     1801 tacgggaact tctgtcccgt ttcgcgatcg acgaacgtct gaaagccgag accgtggccg
     1861 agctcgtgga tgatgaccgt cgcgaagtcg gtcgttccgc ttgtcggctg cgcgtcgagg
     1921 ccgtagtacc acttcttcgg gtacgcgcag ctgccgtcgt cgagagagct tgtgaacgtc
     1981 gccctgacat cctcgtcatc aggatcgagg tcctccccgg cgagcttgtt tgcgagggcg
     2041 acaggaaacc acgtctcggc tcgtggtgcg ccccggaagt ctgaccagac ctgcgtcgtt
     2101 ccagcagatc cgagaacagc gctcgtcgtg gtacagggcg tgagggcctc gaacttcgcc
     2161 cggacacgaa tctcgactgg cgagtcgagt cgttcagccc agaaggcggc cgcgtattcg
     2221 aataccttca agcgctgctc cccgagcgtc gtcccggtgt ttccgccgac gggcgtcctc
     2281 ggtgacgggt cgttgaaccc gagtccggga gcgtcgccat tcacgatggt gatcgtcgtc
     2341 ccatgagcgc gggcggagag gaacagcgcg gcgacgacgg cggcgctgcg aaggagctta
     2401 ctgccggtca tggcggcgca ccgtctcggg ggccggaggc tgtccggcgg ttgcgggctg
     2461 ctccgcggac gtgagcgaga ccttgcgttc ggggccgacg gtatcgacca tttggctgtc
     2521 gaaacgccga tcgaggatga ccctcttcgt gcctctcttc gagtcgatca gctcgactct
     2581 tggcatcggc agagaagtga ccggcgacgc ctcagcagct gtcttcgggg agagcccggg
     2641 cttctggacg aactcgccgg tcacggggtc gatatgtgcc ttcatggcgg cctcggcggc
     2701 cggggcgggc ctgggggctt cgctcttctt ctcggtcgcg gcgaccggga
//
LOCUS       DAOONL010000261         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_50375_length_2543_cov_11.475482, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000261 DAOONL010000000
VERSION     DAOONL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50375_length_2543_cov_11.475482"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="ccsA"
                     /locus_tag="PLP50_17850"
     CDS             <1..543
                     /gene="ccsA"
                     /locus_tag="PLP50_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPA53460.1"
                     /translation="GTPWLAIHVPIIMVGYAVLALGIFVAHMQIGFAAFGRGRGALVE
                     KLYELLYWYMHVGSIFLLAGILTGSVWAASSWGRYWGWDPKEVWSLVAFLAYMAILHL
                     KVDKVIGTFGAAVISVLAFQTILMTYLGVNYVLNVGMHSYGMGDSPIVKWMVVVALVE
                     VVFLVVCGVAFRDRKSVAHG"
     gene            757..2502
                     /locus_tag="PLP50_17855"
     CDS             757..2502
                     /locus_tag="PLP50_17855"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPA53461.1"
                     /translation="MTLIAAAGWVDSAELLVRDALLRTRPRSAAESVAAVVIDEEAID
                     AVGPWPWDRERLAALVDRIGAAGAAAVAVDLLLAESRPGDQALATALAARPSVLAAAP
                     DGTKRWILPASGLRAASEVAHVSFAVDRDGVVRGFLATRQLDGLSLGAMPFAAARLLR
                     PEIPLPVGAPIRPGFRSANEVPTWSAADVLSGRLPEDALRGRVCFVGSVAAGVGDRVV
                     SPLSERGTPDPGVLVQAAATESLLTGDLYERLSPFVAGGLAGLLTGGATALRRRVAPR
                     LALAAVAVPLLAVPAGMVSLLWAGRELPLLATALGGLAATAGPELWRLLEDRRRAATA
                     ARRIVELEALAATLESERKDDAEARRVLAHELKTPLTSVRGLAQLLSGFDLGEERRRS
                     IAAMVARESSRLAEMVETLLDLERIKLKDFRKVSALVELSALVQERTALLASGGGSEI
                     RFSGEPGAVVRGDATLLARVVDNLVGNALKFAPGEAVDVTVDQAGGESVCLAVRDRGP
                     GIPPAERAEIFRRFARGGSAATVPGLGLGLALVAEITRWHGGRVDVEAPDSGGSLFRV
                     TLPAAAAGADPRRRT"
BASE COUNT          300 a          898 c          925 g          420 t
ORIGIN      
        1 gggacgccgt ggctcgcgat ccacgtcccg atcatcatgg tcggctacgc cgtcctcgcc
       61 ctcggcatct tcgtcgcgca catgcagatc ggcttcgccg cgttcggcag ggggcgcggc
      121 gcgctcgtcg agaagctcta tgagctcctc tactggtaca tgcacgtcgg atccatcttt
      181 ctcctcgcag ggattctgac cggctccgtc tgggccgcct cgtcgtgggg ccgctactgg
      241 ggctgggacc cgaaggaggt ctggtcgctc gtcgcgttcc tcgcgtacat ggcgatcctc
      301 cacctgaagg tcgacaaggt gatcgggacg ttcggggccg cggtcatcag cgtcctcgct
      361 ttccagacga tcctgatgac gtacctcggc gtgaactacg tactgaacgt cggcatgcac
      421 tcctacggca tgggcgattc gccgatcgtg aagtggatgg tcgtcgtcgc gctcgtcgag
      481 gtcgtcttcc tcgtcgtctg cggggtcgcc ttccgcgacc ggaagagtgt cgcccatgga
      541 tgagtccggg ggaagagtca ggccgggacg aacacgaacg ggggtcgtgc ctcgaccgcg
      601 cctcgccgga ggtgctctgt cgcctgcacg aaccgcacga ggaacccgca ttcctaaggc
      661 ggttctaatg tccgtggacg taccctcccg gtggtgaagg cgacccggat tcgggcggct
      721 gaaggtctcg gcccgccgct ggcggctgcg tccctcgtga cgctcattgc cgctgcgggc
      781 tgggtcgatt ccgccgagct ccttgttcgg gacgcgctcc tgcggacccg gcccaggtcg
      841 gccgcggagt ccgtcgcggc ggtcgtaatc gacgaggagg cgatcgacgc tgtcgggccg
      901 tggccttggg accgggagcg cctggccgcc ctcgtggacc ggatcggggc ggcaggggcc
      961 gccgccgtgg cggtcgatct cctcctcgcg gaatcccgcc cgggcgacca ggccctcgcc
     1021 accgcgctcg cggcaagacc gtcggtcctc gccgcagcgc cggacgggac aaagcgatgg
     1081 attctccccg cttccggcct gcgcgcggcc tcagaggtgg cgcacgtcag cttcgccgtc
     1141 gatcgtgacg gcgtcgttcg cggcttcctt gcgacccggc agctcgacgg cctctccctc
     1201 ggcgcgatgc cgttcgcggc ggcccggctc ctgcggccgg agatcccctt gccggttggg
     1261 gcaccgatcc ggcccggctt ccgctccgcg aacgaggtgc cgacctggag cgccgcggac
     1321 gtgctgagcg ggaggctgcc ggaggacgcg ctccggggcc gggtctgctt cgtcggctcg
     1381 gtcgcggctg gggtcggaga ccgcgtcgta tcgcctctat cggagcgcgg aacgcccgat
     1441 ccgggcgtcc tcgtgcaggc tgcggcgacc gagtcgctcc tgacggggga cttgtacgaa
     1501 cggctctctc cgttcgttgc cgggggactc gccggcctcc tgacgggggg cgcgacggct
     1561 ctgcggcggc gggtcgcgcc tcgtctggct ctcgcggcgg tcgccgtccc cctgctcgcg
     1621 gttccggccg ggatggtgtc gctcctctgg gccgggcggg agctgccgct cctcgcgacg
     1681 gcgctcggcg gcctcgccgc gacggcggga cccgagctgt ggcgcctcct cgaggaccgc
     1741 cgccgggccg caaccgcggc ccggcgcatc gtcgagctcg aggcgcttgc cgccacgctc
     1801 gagagcgagc gtaaggacga cgcggaagcg cgtcgcgtcc tcgcgcacga gctgaagacg
     1861 ccgctgacgt cggtgcgggg gctcgcgcag ctcctctccg gattcgacct gggcgaagag
     1921 cgccgccgct cgatcgctgc gatggtcgcc cgggagagct cgcgcctcgc ggagatggtc
     1981 gaaacgctcc tggacctcga gcggatcaag ctgaaggact tccgaaaggt ctccgcgctc
     2041 gtcgagctgt cggcgctcgt ccaggagcga acggcgctcc ttgcctcggg gggcggatcc
     2101 gagattcgtt tctcgggtga gcccggcgcc gtcgtccgcg gcgatgccac gctcctcgcc
     2161 cgcgtcgtcg acaacctcgt cggcaacgcc ctgaagttcg cgcccggtga ggcggtcgac
     2221 gtgacggtgg accaggccgg cggggaatcg gtatgcctcg cggtgcgcga ccggggtccg
     2281 ggcatcccgc ccgccgagcg agcggagatc ttccggcgat tcgcccgcgg gggctccgcg
     2341 gcgacggtcc cggggctcgg cctcggcctc gcgctcgtcg ccgagatcac gaggtggcac
     2401 ggtgggcgcg tcgacgtcga ggctcccgac tcggggggaa gcctcttccg cgtcacgctt
     2461 cccgcggcgg ccgctggcgc cgatccccga aggagaacgt agatgccgcg tgccctcgtc
     2521 atcgacgacg acgacgccat ccg
//
LOCUS       DAOONL010000262         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_53047_length_2452_cov_14.140175, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000262 DAOONL010000000
VERSION     DAOONL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53047_length_2452_cov_14.140175"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLP50_17860"
     CDS             complement(<1..639)
                     /locus_tag="PLP50_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPA53462.1"
                     /translation="MKLGIVGRPKTGKTTLFNLLTRSHQSVDKFGAVRETHVGTAHVP
                     DPRLDRLSAMFKPKKHTPATVDFVDVPGIAKGEQEKIDLAALKNVEALVHVVRAFEDA
                     DIPHEGGLDPARDAKALDEELVLSDYVLLEKRREKLEKDRKKTKSAELDAEWAAIERC
                     LPSLESGTPLRAAGLTAEEEKRLRGFTLLSLKPLLIVVNVSDADASAEPVARF"
     gene            complement(683..2299)
                     /locus_tag="PLP50_17865"
     CDS             complement(683..2299)
                     /locus_tag="PLP50_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA53463.1"
                     /translation="MTPNPLRTRALKLLAAPLVLAATTLLGGTFGPRLLAVSDATNPV
                     LKEYSDLLATAQAWYADDLGSERLVYSSISGMLDTLDPHSSFLEPEEYGAMQEKQRGS
                     FYGLGIIIAKRNGRVTVITPVEGSPAQRLGIRAGDIIDLVEGQPIDDLPIDAVVKKLK
                     GPKGTKVNITIVRPGLGEPLQMTVTRAEIPTNSVSFAFMVEPEVGYIRLKDFTHTSAP
                     EVAEAWAKLEKQGMKKLVFDLRANPGGLLDQAIAVSDFFLRKGEKVVITRGRAAGSEQ
                     TFTAPGRNSRARIPVVVLINKGSASAAEIVAGAIQDQDRGIVAGQTSWGKGLVQSVYT
                     LSEGAGLALTTAKYYTPSGRCIQRDYREFIEYVAPDDEEESNPLLAEDGVKRESYRTA
                     SGRVVYGGGGITPDVVVKADRLSRWMATLYARGVFFEWAVQYRASHPEVPRDFAVTDA
                     VREDFFAHLDGRPNAPDVPARASWAAEKDKAVLDRALAEELVGAVHGPEAAYRLSIGG
                     DVQLKKALTLFPEAEKLAGIGTTPEAIAKR"
BASE COUNT          333 a          824 c          896 g          399 t
ORIGIN      
        1 gaagcgcgcg accggctccg ccgaggcgtc ggcgtccgag acgttcacga cgatcagcag
       61 cggcttgagc gagaggagcg tgaagccgcg gagccgcttt tcctcctcgg cggtcaggcc
      121 tgccgcccgg agcggagtcc ccgattcgag ggaggggagg cacctctcga tcgccgccca
      181 ctcggcgtcg agctcggccg acttcgtctt cttccggtcc ttctcgagct tctcgcgccg
      241 cttctcgagg aggacgtagt ccgagaggac gagctcctcg tcgagcgcct tcgcgtcgcg
      301 cgcggggtcg agacccccct cgtgcgggat gtcggcgtcc tcgaacgcac ggacgacgtg
      361 gacgagcgcc tcgacgttct tcagcgccgc caggtcgatc ttctcctgct cgcccttcgc
      421 gatccccggg acgtcgacga agtcgaccgt ggccggcgtg tgcttcttcg gcttgaacat
      481 cgccgagagc cggtcgagtc gcgggtcggg gacgtgcgcc gtgccgacgt gcgtctcgcg
      541 gacggcgccg aacttgtcga ccgactggtg ggagcgggtc aggaggttga agagagtggt
      601 ctttccggtc ttcggccggc cgacgatccc gagcttcatg gccgcgggag cgtagcagaa
      661 cgtcccgcgg ccgcccgtcc ggctacctct tcgcgatcgc ctcgggggtc gtgccgatcc
      721 cggccagctt ctcggcctcc gggaagagcg tcagcgcctt cttcagctgg acgtcgccgc
      781 cgatcgagag gcggtaggcc gcctcgggtc cgtggaccgc cccgacgagc tcctcggcga
      841 gggcgcggtc gaggacggcc ttgtccttct cggcggccca cgacgcccgc gccggcacgt
      901 ccggggcgtt ggggcggccg tcgaggtggg cgaagaagtc ctcgcgcacc gcgtcggtca
      961 ccgcgaagtc ccgggggacc tccggatggc tcgcccggta ctggacggcc cactcgaaga
     1021 agacgccgcg cgcgtaaagg gtcgccatcc accgcgagag cctgtcggcc ttcacgacga
     1081 cgtcgggcgt gatgcctccg ccgccgtaga cgacccggcc gctcgccgtc cggtaggact
     1141 ccctcttcac gccgtcctcg gcgaggagcg ggttcgattc ctcctcgtcg tccggggcga
     1201 cgtactcgat gaattcccgg taatcccgct ggatgcagcg acccgacggc gtgtagtact
     1261 tggccgtggt cagcgcgagg ccggccccct cggagagcgt gtagaccgac tggacgaggc
     1321 ccttccccca gctcgtctgg ccggcgacga tgccgcggtc ctggtcctgg atcgcgccgg
     1381 cgacgatctc ggcggccgac gcggagccct tgttgatcag gacgacgacc gggatgcggg
     1441 cgcgggagtt ccggccgggc gccgtgaagg tctgctcgga gcccgcggcg cggccgcgcg
     1501 tgatgacgac cttctccccc ttgcggagga agaagtcgga aaccgcgatc gcctggtcga
     1561 ggaggccgcc cgggttggcg cgcaggtcga agacgagctt cttcatcccc tgcttctcga
     1621 gcttcgccca cgcctcggcg acctcggggg cggaggtgtg cgtgaagtcc ttcagtcgga
     1681 tgtacccgac ctccggctcg accatgaagg caaacgagac ggagttcgtc gggatctcgg
     1741 cgcgcgtgac ggtcatctgg agaggctcgc cgaggccggg ccggacgatg gtgatgttca
     1801 ccttcgtccc cttcggtccc ttcagcttct tcacgacggc gtcgatcggg aggtcgtcga
     1861 tcggctgtcc ctcgacgagg tcgatgatgt cgcccgcgcg gatcccaagg cgctgcgccg
     1921 gcgagccctc gacgggcgtg atcaccgtga cgcggccgtt ccgcttcgcg atgatgatcc
     1981 cgagcccgta gaacgacccg cgctgcttct cctgcatcgc tccgtactcc tccggctcga
     2041 ggaagctgga gtgcgggtcg agcgtgtcga gcattccgga gatcgaggag tagacgaggc
     2101 gctcggagcc gaggtcgtcg gcgtaccagg cctgcgcggt cgcgaggagg tcggagtact
     2161 ccttgaggac cgggttcgtc gcgtcggaga cggcgaggag gcgggggccg aaggtcccgc
     2221 cgaggagcgt cgtcgcggcg aggacgagcg gggcggcgag cagcttcagg gcgcgggtgc
     2281 ggagcgggtt cggcgtcacg aaggcctccg cggcggacgg tagcacgtgg cgtgccggag
     2341 gcgggagagg gccgagctcg cgcgcgacgc gccgccgggc gcgggccggc acgtcgtcct
     2401 gtcatggctc cttagaaatg tcaccccctg gaactcgata gaaatgtccg tt
//
LOCUS       DAOONL010000263         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_53966_length_2423_cov_8.823480, whole genome shotgun sequence.
ACCESSION   DAOONL010000263 DAOONL010000000
VERSION     DAOONL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53966_length_2423_cov_8.823480"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLP50_17870"
     CDS             complement(<1..449)
                     /locus_tag="PLP50_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPA53464.1"
                     /translation="MNKKRILTITLTVALSVVAGGVVLVSSACQKSGEHSAAAKRFHC
                     PMHPDVVKDGPGDCPICGMKLVPIEEEAHQAAVAGAAGPSGERKILFYRSPMNPQETS
                     PTPKKDSMGMDFVAVYSDEVNSGEKGPEGLATVTIDAQKQQLLGLKT"
     gene            complement(458..1792)
                     /locus_tag="PLP50_17875"
     CDS             complement(458..1792)
                     /locus_tag="PLP50_17875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPA53465.1"
                     /translation="MSILWSRRGVALCALLAVAAFKTQGVEPPPPLDRAEVERLVEEA
                     LEANPEIRSARATAASAEARIKPAGTLPDPMVSLSYENDGVSPSLGTMEMTRLQFMAQ
                     QAIPYPGKLRLAREVAEKDAERAGTAPQRVVLTIGASVRRAYADLLEAREGLRLVDEQ
                     AETWKGIEEVTRGRYAAGLASQQDVLRAQSERTRLLQERRREEAAELTALSELRQLLF
                     RPPDAPIPTEQRLTPGRLVTIPSSAETLARAVEETPELKEIALVRERSKLSVDLARRN
                     LRPDFVASAAYMNRGGLPLMWSAGLGVSIPLWAGQKQRPLIVEAETLASAAAATEESL
                     RRRVQAATEERLIRLNQLAQEARLDAEGILVQDQLSVDAALASYRTGTVPFVTVLEAI
                     GTYFGDRRAALGRLANLIRALADLEEFSPERSSQAPMASKTAPAAASASPKM"
BASE COUNT          369 a          838 c          820 g          396 t
ORIGIN      
        1 atcgtcttga gacccagcag ctgctgcttc tgggcgtcga tcgtgaccgt ggcgagaccc
       61 tccggtccct tttcgcccga gttcacctcg tccgagtaga cagctacgaa gtccatcccc
      121 atagagtcct tcttcggggt gggcgacgtc tcctgcggat tcatggggga ccggtagaag
      181 aggatcttcc tttccccgga gggtccggcc gctcccgcca ccgcggcctg gtgggcttct
      241 tcctcgatgg ggacgagctt catgccgcag atcgggcagt cgccgggacc gtccttgacg
      301 acgtccgggt gcattgggca gtgaaaccgc ttcgcggcag ccgaatgttc accgctcttc
      361 tggcacgcgg acgagaccag gacgaccccg ccagcaacga cagacagagc gacggtgagg
      421 gtgatcgtga ggatgcgctt cttgttcatt tctctgctca catcttcggg ctggccgagg
      481 cggccgccgg ggctgtcttg gacgccatgg gcgcctggct actccgctcg ggagagaact
      541 cctcgaggtc ggccagtgcg cggatcaggt tcgccagccg tccgagggcg gctcggcgat
      601 cgccgaagta cgtgccgatc gcctcgagca ccgtcacgaa ggggacggtg ccggtccggt
      661 agctggcgag ggcggcatcg acggagagct ggtcctgcac gaggattccc tcggcgtcga
      721 gccgagcttc ctgggcaagc tggttgaggc ggatgaggcg ttcctccgtt gcagcctgaa
      781 ctcggcgacg gagggactcc tctgtggcgg ctgccgccga ggcgagggtc tcggcttcca
      841 cgatgagcgg ccgctgcttc tggccggccc agagaggaat cgagacgccg agaccggcgg
      901 accacatgag cgggagcccc ccgcggttca tgtatgcggc cgaggcgacg aagtcgggtc
      961 tgaggttccg gcgcgcgaga tcgacggaga gcttggaccg ttccctcacg agtgcgatct
     1021 ccttcagctc cggggtctct tcgacggccc gggcgagggt ctccgcgctc gacgggatcg
     1081 tcacgaggcg tcccggtgta aggcgctgct cggtggggat aggcgcatcc ggcggtcgaa
     1141 agaggagctg acgaagttcg ctcagggccg tcagctcggc tgcttcctcg cggcggcgct
     1201 cttgaagaag ccgcgtccgc tcgctctgcg ctcggaggac gtcctgctgg ctcgccagac
     1261 ccgctgcgta ccgacctcgg gttacttcct cgatgccttt ccacgtttcg gcctgctcgt
     1321 ccacgagccg caagccttcg cgtgcctcga ggaggtccgc gtaggcgcgg cggaccgaag
     1381 ccccgatcgt gagcacgacg cgctggggtg cggtccccgc ccgctcggcg tccttttccg
     1441 cgacctcccg ggcgagacgg agcttccccg ggtacggaat cgcctgctgc gccatgaact
     1501 ggagacgcgt catctccatc gtccccagcg agggcgacac cccgtcgttc tcgtagctca
     1561 gcgagaccat tgggtccggc agggtgccgg caggcttgat ccgcgcctcc gcagaggctg
     1621 ccgtggcccg cgccgaccga atctcgggat tcgcctcgag cgcctcctcg acgagacgct
     1681 ccacctcggc gcgatccagc ggcggcggag gctctacccc ctgggtcttg aatgcagcga
     1741 cggccaggag ggcgcaaagc gcgactcccc gtctggacca taggatggac atctcttcac
     1801 ctcgtgctcc ggtggtcggg cgccgcctgc gtcaggcagc gacgaaaagg ccacgcccca
     1861 ggatcagatc ctgagaatgc cgatccggag gaagagcagg ccgtccccgg gccggcgccg
     1921 caccagtgtc cgcaggttct ggcgcgccga accaaggtcc aggtcaactt cccgacccgg
     1981 cgactccgag accccggacc gagacgcacc ttcgggcggc gccgcttccg gccccccggg
     2041 gtcccgggtg acgctgcaag gccccccagg ggggcagcag catccccctg aatcgtccga
     2101 cagagtgtcc cccggggttc ccgccgggcg aaccgcgcca ccgcagcaat cccccgggct
     2161 ccccgatgcc gcgcttccca gccagcgggc cgcggtcgcc gcgcaggtag ccggcgcgac
     2221 gttcgcgccg agcaggagca cggagagagc cagagccgcc ccacgataga accagcgccc
     2281 ccgattccca cccctggcga tctcggggga catctgcgtg cgggctgcgg ggttttcctg
     2341 gcgaaacacc atcgactctg ctcgcaggga acttaccccc ccaacattag aaacggatta
     2401 gtgccgcggt atcgtgagac cct
//
LOCUS       DAOONL010000264         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_54095_length_2419_cov_12.323181, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000264 DAOONL010000000
VERSION     DAOONL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54095_length_2419_cov_12.323181"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLP50_17880"
     CDS             <1..821
                     /locus_tag="PLP50_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPA53466.1"
                     /translation="PLAAAIVKGAEKRGVALSGTDGFESVTGKGVKGTVDGRAVTLGN
                     TSLMADLGIDLGALGTDAEALRKDGETAMFVAVDGEVAGILGVADPIKASTPEAISEL
                     HGEGLRIVMLTGDSRTTAEAVARKLAIDEIVAEVLPDQKAEVVKRFQAQGRVVAMAGD
                     GINDAPALAQAQVGIAMGTGTDVAMESAGVTLVKGDLRGIVRARRLSRSTMANIKQNL
                     FFAFVYNAAGVPIAAGVLYPFFGLLLSPVIAAAAMSFSSVSVVGNALRLRRAKM"
     gene            848..2335
                     /locus_tag="PLP50_17885"
     CDS             848..2335
                     /locus_tag="PLP50_17885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPA53467.1"
                     /translation="MPGGERSRPGRPDRAAPRYELGETLGSGGMATVVLARDRQLGRD
                     VAIKRMKPELAEDPGARRRFEEEALILAGLEHPGTVPVHDAGTLETGEPFYAMKRVQG
                     RTLKSLLSARRPEEIRSRQSITHFVDVFERVCQTLAAAHRQGLVHRDVKPANIMVDDM
                     GSVYLVDWGLAVRLEGDSQSRAHPGGAVAGTPAYMSPEQARAAPTSPASDVFSLGTVL
                     YEILTGANPFSSSDALESMRRVREESPAPPVASNPVAGRSLSAVCMKALARNPSSRYD
                     SAMELAEEIRRYREFRPVGAAPPTFMERAVNWMRRHPAVASAVAAALGVGLLGGVGVA
                     VRVASRHSLHVMASERLDAADASLARVNAEIARATAAREAAPSNGRARLDLELDELRA
                     LRSEEYETVHGLAYAILGFGAGSPDRVIQARVRRYQVEGVERYLAAGQELAAAASLRK
                     ALRAAEAANPLGFSKDEIAAMEAKLMQVEARLAASRGPESEPAGH"
BASE COUNT          394 a          786 c          885 g          354 t
ORIGIN      
        1 acccgctcgc ggcggccatc gtgaagggag ccgagaagcg cggcgtggct ctctccggca
       61 cagacgggtt cgagtcggtg acgggaaagg gcgtgaaggg aaccgtagac ggcagggccg
      121 tgaccctcgg gaacacgtcc ctgatggcgg acctgggcat cgacctcgga gcgctgggca
      181 ccgacgcgga ggcgctccgc aaggacgggg agacggcgat gttcgttgcg gtcgacggcg
      241 aggtcgcggg catcctgggg gtggccgatc cgatcaaagc cagcacgccc gaggccatca
      301 gcgaactaca tggtgagggc ctccgcatcg tcatgctcac gggcgacagc aggacgaccg
      361 ccgaggcggt cgcgaggaag ctcgcgatcg acgagatcgt ggcagaggtt ctgcccgatc
      421 agaaggcgga ggtcgtgaag cggttccagg ctcagggacg cgtcgtcgcg atggcaggcg
      481 acggcatcaa cgacgccccc gcgctcgcac aggcgcaggt gggaatcgcg atggggacgg
      541 gcaccgacgt cgcgatggag agcgccgggg tcactctcgt gaagggggac ctccggggca
      601 tcgtccgggc gcggcgcctt tcccgcagca cgatggccaa catcaagcag aacctcttct
      661 tcgcattcgt ctacaacgcg gcaggggtcc cgatcgctgc gggtgtgctc tacccgttct
      721 tcgggctatt gctctcgcct gtgattgcgg ctgccgcgat gagcttcagc tcggtctcgg
      781 tcgtcggaaa cgcgctccgg cttcgacggg cgaagatgtg agcctggacg acgagacgat
      841 tgaggcggtg cccggcggcg agagatcgcg ccccggtcgc cccgatcgcg cggctccgcg
      901 ctatgagctc ggcgagacgc tcggcagcgg ggggatggcg acggtcgtgc tcgcgaggga
      961 tcgccagctc ggacgcgacg tcgccatcaa gcgcatgaag cccgagctcg ccgaggaccc
     1021 gggggcacgg cgccggttcg aagaggaagc gctgattctc gcggggctcg agcatccagg
     1081 gacggtcccg gtgcacgacg cggggacgct cgaaacgggc gagccgttct acgcaatgaa
     1141 gcgcgtccag ggacggacgt tgaagagcct cctgtcggcg cggcggccgg aggagatccg
     1201 gtcgcgccag tcgatcaccc acttcgtcga cgtcttcgag cgggtttgcc agacgcttgc
     1261 cgcggcgcac cgccaaggcc tggttcatcg cgacgtcaag ccagcgaaca tcatggtgga
     1321 cgacatgggc tccgtctacc tggtggactg gggcctcgcc gtacggctcg aaggcgacag
     1381 tcagtcccgc gcgcatccag gaggggccgt tgccgggacg ccggcgtaca tgtcccccga
     1441 gcaggcgcgc gcggcaccga cgagcccggc ctccgacgtc ttctcgcttg gcacggtcct
     1501 gtacgagatt ctgaccggtg cgaacccctt ctcgtcctcc gatgcgctcg aatcgatgcg
     1561 ccgcgttcgg gaggagtcgc cagcgccgcc cgtcgcgagt aaccccgtag ccgggagatc
     1621 gctctctgcc gtctgcatga aggccctcgc caggaacccg tcgtcgcgat acgactcagc
     1681 aatggagctg gcggaggaga tccgccggta ccgggagttc cgtccggtgg gcgctgcgcc
     1741 tccgaccttc atggagaggg ctgtgaactg gatgcgccgg caccctgcgg tcgcctcggc
     1801 cgtcgcggcc gcgctcggcg tcggcctcct tggaggagtc ggggtcgccg ttcgcgtcgc
     1861 ttcccgccac agtctgcacg tgatggcttc ggagcgcctg gacgccgcgg acgcgagcct
     1921 cgcccgagtc aatgccgaga tcgctcgcgc caccgcggcc cgcgaagccg ctccgtcaaa
     1981 cggccgagcc cggctcgacc tcgagctcga cgagctgaga gccctgaggt cggaggagta
     2041 cgagaccgtt cacggattgg cctacgccat tctcgggttc ggggcgggct cgccggacag
     2101 ggtcatccag gcgcgagtcc gccgatatca ggtcgagggc gtcgaacgct acctcgccgc
     2161 tggccaggag ctcgctgcag cggcgtcact gaggaaggcg ctcagggccg ccgaggcggc
     2221 gaatccgctt ggcttctcga aggacgagat agcggcgatg gaggcgaagc tgatgcaggt
     2281 cgaggcgagg ctggcagcct cccgcggacc cgaatcagag ccggccggac actgaagagc
     2341 cgccgtcggc atggcgtacc accatagagg tgcggccgga ggtctgaacg ggccccagcg
     2401 tcgcgagatt actcgcagg
//
LOCUS       DAOONL010000265         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_2650
            NODE_58016_length_2304_cov_15.871054, whole genome shotgun
            sequence.
ACCESSION   DAOONL010000265 DAOONL010000000
VERSION     DAOONL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864725
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 14:04:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,580
            CDSs (total)                      :: 3,548
            Genes (coding)                    :: 3,519
            CDSs (with protein)               :: 3,519
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 2 of 29
            Pseudo Genes (incomplete)         :: 28 of 29
            Pseudo Genes (internal stop)      :: 0 of 29
            Pseudo Genes (multiple problems)  :: 1 of 29
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58016_length_2304_cov_15.871054"
                     /isolate="AMR_MDS_2650"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..551)
                     /locus_tag="PLP50_17890"
     CDS             complement(54..551)
                     /locus_tag="PLP50_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA53468.1"
                     /translation="MSDADRRSGTRASALLLFVAGFLLPASTPLHAGTVTGTVRNSST
                     NATLSGVLVKVNQQSGRQATTNSSGVYRINSVSAGTVTLALSKSGFVSATSSPITVPA
                     SGTVTVPQILLEPLGTITGTVVNSTGGAGVAYAKVTVTGTATQATTSSSGSFSLSRET
                     SVGGC"
     gene            1484..1792
                     /locus_tag="PLP50_17895"
     CDS             1484..1792
                     /locus_tag="PLP50_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA53469.1"
                     /translation="MNTKRIRTALAGALLSLALFAPAAEAARVVVKVGPPAARVEVRA
                     GAPSPRHVWVGGYWRWDGRAHVWVAGGWQLPPRHRAVWVDGHWKKVRGGWTWVPGHWR
                     "
     gene            complement(1967..>2304)
                     /locus_tag="PLP50_17900"
     CDS             complement(1967..>2304)
                     /locus_tag="PLP50_17900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA53470.1"
                     /translation="ARVKQVVLNLVGNALDAVEAVGARAREVDVAVEDGGRFWRLTVA
                     DHGPGIDGGGSGDVFRVFVSTKPAGTGMGLPIAERIVHAHGGELTLASPSGGPTRAVA
                     TFPKHDAPG"
BASE COUNT          370 a          816 c          783 g          335 t
ORIGIN      
        1 ttcgtgtctg caactcgatg gtacgcagag gcttcagaac gatgcttctg ttttcagcac
       61 cctcctactg aggtttctcg gctgaggctg aagctgccgg aggagctcgt cgtcgcctgc
      121 gtggccgtgc cggtcaccgt caccttggca taggcgacgc ccgctccgcc ggtggagttg
      181 acgacggtgc cggtgatcgt tccgagcggc tcgaggagaa tctgcggcac cgtgaccgtc
      241 cccgaggccg ggaccgtgat cggcgaggac gtcgccgaca cgaagcccga cttcgagagg
      301 gcgagagtga cggtgcccgc cgaaaccgag ttgatccggt agacgccgga cgagttggtc
      361 gtcgcctgcc gcccggactg ctggttcact ttgaccagga cacccgacag cgtcgcgttc
      421 gtcgacgagt tccgcacggt tcccgtgacg gtcccggcgt ggagcggcgt cgacgcgggg
      481 aggagaaacc ccgcaacgaa gaggagaaga gccgaggctc gcgtacccga ccgacgatcc
      541 gcatcactca tggcgcaact ccgctttcgt gtccctctgg caacctggag gacgtcgttg
      601 tctgggacag caggctacgg agcccgacga cgagaggtca acggtcgccg cgaagaaatt
      661 cgggacaatc ccggacacga cgcacggccc aggccccgcg agctcgggac tccgcccccc
      721 tgctcgttac gaaacgcctt cgatgcgcgg tcgccacgtt gcccgcgtcg tcccccgaac
      781 ggattcggag cagccgggga gatcggcgct catccggacg caccgccgga ggtcccgcaa
      841 cgagcccggg aacgtcgatc gaacccaccg acggtacgcg gaggagctcg agaaccccag
      901 ccaggctgcg accacgctcg tcggcacggg gacggtgagc ccagccgccg tcatccggcg
      961 gcggactcga tcgacgaact ccttcggccg gcaatccgcc gccaggagtc tcctgcggat
     1021 cgtccggctg gaacacccca ggaagcgagc aagggcctcc actcccgagg tcaccaggac
     1081 gagcgcgccc tccacgtcca gagtcccggc caacgccgct tcctcgagga cgatcgcccg
     1141 gaataccgcg aaggcggggt tgtggatgcg cgggccggtc cgggtcgaag tccacggaat
     1201 ccgatcgttc cgctgcccaa tcgcttgtcc gtagacgggg ttgatgagaa cagagtgcaa
     1261 ttccggcacg cgaagccctc cctctactct tgatgagtgt tcgagagagg ctctactgcc
     1321 ccttcgcgaa aaaaaagttg aaggagcctc tccccgaaga ccgattcggc gagtcacgag
     1381 ggatctgcag tgatctccct caccctctcg ccacccgccg ccaacccgcc gcgcacggtc
     1441 ggcgtccatt ccctcgagac gcagctcgaa ggaggactcg acgatgaata cgaagcgcat
     1501 ccgcactgcc ctggccggcg cccttctctc cctcgccctc ttcgcccccg ccgcggaggc
     1561 ggctcgagtc gtggtcaagg tcggcccgcc ggccgcgcgg gtcgaggtac gggcaggcgc
     1621 cccttcgccg cgccacgtct gggtcggcgg ctactggcgc tgggacggcc gagcgcacgt
     1681 ctgggtcgcg ggcggctggc agcttccgcc acggcaccgg gctgtctggg tcgacggcca
     1741 ctggaagaag gtccgcggcg gctggacctg ggtgcctggc cattggaggt gagccctccc
     1801 ggccgaaaag gaggagaatc gaggcgaggc ggccttcccc ggcagcttcc ccgggcggcc
     1861 cccccggaac gcccgccgat ctcgaaccga gccggcgggc gtcgttgcgt ccgggagatg
     1921 gcggccgcgc gagtcgcgct cgtctcgccg cgtgaggcga cgagcctcag ccgggagcgt
     1981 cgtgcttcgg gaaggtcgcc acggcgcggg tcggcccgcc agacggggag gcgagggtca
     2041 gctcgccgcc gtgcgcgtgg acgattcgct ccgcgatcgg gagccccatg ccggttccgg
     2101 ccggcttcgt cgagacgaag acgcggaaga cgtcgccgct ccccccaccg tcgatgccgg
     2161 ggccgtggtc cgcgaccgtg agacgccaga agcgtccgcc gtcctcgacc gccacgtcga
     2221 cctcgcgcgc cctcgccccg acggcctcca cggcgtcgag cgcgttcccg acgaggttca
     2281 ggacgacctg cttgacccgc gccg
//
